LOCUS       DASYCS010000001         8209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4349218,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000001 DASYCS010000000
VERSION     DASYCS010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 8209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8209
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4349218"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..98)
                     /locus_tag="VGM46_00005"
     rRNA            complement(<1..98)
                     /locus_tag="VGM46_00005"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            891..2849
                     /locus_tag="VGM46_00010"
     CDS             891..2849
                     /locus_tag="VGM46_00010"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY4191003.1"
                     /translation="MHDTQEAIAELGNQPPLIADGRSGPPDRREVSARWLSGTFLTGL
                     TSSVLMGVALVAALDGRQQLATPPEIAELISLAKGGEAGEGAKTLRLVPLRQIARAKD
                     RRRMEISMVTKVGDRDVIHTVPFVQIKMNLAAGHTTTRPYPAFDPMQVFGDDTPDQPA
                     SPVLGSGQIYGAKVESEMSLKTVDYPIATAAFDERNDLSANEVEKVVREAGADLDDGA
                     IHVAALHYVDPQRFGDQLAQSMAGSYGVKIVPENVSVSPRADAEDHAVSFAEEIIPFT
                     KDYGIADAFADSGYTGEDATGMADAIGKLLNAPALKAGTVLRVGLEVRGETAKVVRTS
                     VYDKTQHIVTIALDDHGQYVPAKEPEANPELLTAFDGSAPVTARGNLPNIYDGIYRAA
                     YSYGMSKTMTKRLVKLLASDVDFQSRLAPSDRIEVLFSQPDGDDQASDDSELLYVSST
                     FGGQTRTLYRFQMQDGDADYFDQDGRSAEQFLLRNPLPAGRFRSGFGARKHPILGYVR
                     MHTGVDWAAPIGTPIIAAGNGVIEKVGWAGGYGKQIMIRHANGYETSYNHQSAFANGI
                     KAGMHVRQGQVIGYLGQTGLATGPHLHYELIVNGTKVDPMRVRLPVGKALKGDDLVAF
                     KRERERIDDLLKQEDADPLKVASAKVEG"
     gene            complement(2950..4494)
                     /locus_tag="VGM46_00015"
     CDS             complement(2950..4494)
                     /locus_tag="VGM46_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4191004.1"
                     /translation="MQTIDKVSEASAGQARPANGASFCPQSSRRFVLIAAILASALGF
                     IDGSVLAIAIPAIRVDLGASLAQAQWISNAYALTLSALILAGGAAGDRFGLRGTFIAG
                     IGLFILASLACALAPNPPVLVLFRAIQGIGAAIMVPCSLAIIAKAYPKNERGRAIGIW
                     AAASALTTALGPVLGGLLIASFNESIWRAIFAINLPLGAVSIYLLFAKVPADKPTEKR
                     ALDLGGAALATLAFGVLAYGLTALNGKGEGGLSGMSIAAGIVLLVVFIGWEIKQREPM
                     IDLGLFRSPAFAGANLSTFFLYFALSANLFYLPMLLIAGWGLSAGEVGFIFLPLSGFI
                     ALLSGRVGRFSDKAGPRLPLAVGSLLVAFAFAGMALLAFAGFHHFWTGIFPLMALLGL
                     GMALVVSPLSTAIVTAVEDKDTGAASGINNAVSRIGGLIAVAAMGSVAAVVYAKTLGG
                     AGGADAPGFGEPVQAGMAHSLDLARMAASDTAFAAVALVTAVLCLLSAIVAWMTIPGN
                     QSGSGA"
     gene            complement(4616..6112)
                     /locus_tag="VGM46_00020"
     CDS             complement(4616..6112)
                     /locus_tag="VGM46_00020"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY4191005.1"
                     /translation="MLRVIVENVPPLDQGKEPSGQFAASLTSVAIFAGVILTAALFGN
                     FTRPAGHLSAFWPANALMLGMLVRNPKLARPFALLGAAGAYMCADLISGGPLVMTLLL
                     TIANMVSVMAGYQLFSRLDPLDQRLGRPLSIFYLVLGAALCAAAAGVVGGIANPLLFQ
                     GSVLEGWGFWFVSELVNYIAILPVILTLPALAWSKDKADVSRYASLNVTPAMLAPAAF
                     LVLSAIAGLVVGGPGAIGFPVPALLWCALVYSQFMTAVLTLLFSGWTLLAISMGYIPL
                     GLDNNEWSTVMSIRMGVTLIALAPVTVASVTAVRNELMRRLQHMAAHDQLTDLLNRGG
                     FKARSQALLGLPSAAKQPVAMLMLDIDRFKDINDTYGHAAGDRVLSAFARLAAAKLRG
                     GDPFGRIGGEEFAILLSDCSRECAEQIAHHIRTVFEQATIDVGDGRSITATVSIGLVM
                     AEKPIVDLDALLLAADKSLYRAKREGRNRVELGELDAGPSMLFDGWRA"
     gene            complement(6258..7727)
                     /locus_tag="VGM46_00025"
     CDS             complement(6258..7727)
                     /locus_tag="VGM46_00025"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEY4191006.1"
                     /translation="MLRTILLLSTFALASLPGMVVAAPSPNDHRDEAVKAPAGNGLLV
                     AQNGDYEVYFDGKGNRVIVDAYTGKVVAIQPPQTKFDRRALRAPQRQRNLRQGSADDS
                     YYLDDPEDMERFRRRRLQEEGGEGSPSVGEYDPGGDDSVGTVPDDGGYGEAYPEEPQA
                     PRDVERRKAAPPLDQASIQPAQPEVLVEPEQPAGADVPRTRVDPSLSLGQREDIAALQ
                     VLMDRAGASPGVIDGRFGANVDKALAAYRQITGSQLKTTDTIGITQALANSGGDAFAS
                     YTITPEDAAGPYLASVPLDYSEKAKLDRMSFTSVAEALAERFHMDENYLKALNPEANF
                     NRPGTVIKVANFGKLAKTQVTNILADKGMKQVRAYDAAGKLVAAYPATIGSTDTPSPS
                     GTHAVSRIALDPNYTYNPKLNFKQGENDKVLTIPPGPNGPVGSVWIALDKPTYGIHGT
                     PDPSKIGKTESHGCVRLTNWDARELARLVKPGVTVEFID"
     gene            complement(7962..>8209)
                     /locus_tag="VGM46_00030"
     CDS             complement(7962..>8209)
                     /locus_tag="VGM46_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009452473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191007.1"
                     /translation="DRKIALQLDADAVEWLAAKGYDPAYGARPLKRVMQKELQDPLAE
                     KILMGDILDGSTVKVTAGSDRLNFRSKPTPIEEAVAA"
BASE COUNT         1622 a         2644 c         2465 g         1478 t
ORIGIN      
        1 actcacccgt ctgccgctcc ccttgcgggg cgctcgactt gcatgtgtta agcctgccgc
       61 cagcgttcgt tctgagccag gatcaaactc tcaagttttg aaactttgat tggctttgat
      121 gttgcaacct aagtcacaac tatctggtca cgcatttgaa ttgacgagaa cattcacacc
      181 tagatacttg cgtatcttgg tagacttatt ctctcaaaac gtgtccgcca aagtctcgtt
      241 cgaaccatct cgtccctggc gggacaaggg gttcagcagg actctgccgc ccacgtttct
      301 ctttcttcat tatttaattt tcaaagaaca gacattgcaa aacaatgtcg aggcctgtcc
      361 gcttgtggct tcgggccgat cgagtgtcgc ttacgcggct ctctttgatt tcgttcaacc
      421 agggcagtct tagaagcgaa cttcttggtc gccagcggcg cgccgccctc gttgttgagg
      481 cgtatatagt cgggacccgc tccaagtgtc aacaacgatt tcgaagtttt ttgaattttt
      541 tgcgacaaaa atcgcgatgc cccgttttgg cagccggctt acgtaggggc cgcaagggtt
      601 tgcccttttc ggcccgttct ccatgcacag gcacgaagcc ctattgccgc ctcattggcg
      661 gtcgacaccc tgcatcggct ttgcaagtgt aggtttccgc cggcgctttc gggccgcgga
      721 cagggccgca atcgccttcg ggcaacaccg tttcgctgcc cgccctatat agagagtaga
      781 tcagcaggga ttcccgaggc cgggtttcgt tgacttaccc gccatcgccg ggcaaatgtc
      841 acggctgcta ttgagtgaca cgcccgcctg ggaagaagcc accgttctgg atgcacgata
      901 cgcaagaggc catagccgaa ctcggcaacc agccgccgct gatagcggat ggccgcagcg
      961 gcccgcctga ccgccgcgag gtttcggcgc gctggctctc cggcaccttt ctcaccggcc
     1021 ttacttccag tgttctgatg ggcgtggcgc tggtcgccgc cctcgatggc cgccagcagc
     1081 tggccacccc gcccgagatt gccgaactca tcagtctggc caaaggcggc gaagccggcg
     1141 aaggcgccaa gacgcttcgc ctggtgccgc ttcgccagat cgcgcgggca aaggatcgcc
     1201 ggcgcatgga aatatcgatg gtcaccaagg ttggcgaccg cgatgtcatc cacaccgtgc
     1261 cgttcgtaca gatcaagatg aacctcgccg ccggccacac cacgacccgg ccctacccgg
     1321 ccttcgaccc gatgcaggtg ttcggcgacg acacgcccga ccagccggca tcgcctgttc
     1381 tcggctccgg ccagatttac ggcgccaagg tcgagagcga gatgagcctc aagaccgtcg
     1441 actacccgat cgcgactgcc gccttcgacg aaaggaacga cctttcggcc aatgaggtgg
     1501 aaaaagtggt gcgcgaggcc ggcgccgatc tcgacgacgg cgccatccat gtggcggcgc
     1561 ttcattatgt cgaccctcag cgcttcggcg atcagctggc gcaaagcatg gccggttcct
     1621 atggcgtcaa gatcgtgccg gaaaacgtct ccgtttcccc gcgcgccgat gccgaggatc
     1681 atgccgtctc cttcgccgag gaaatcatcc cctttaccaa ggactacggc atcgccgatg
     1741 cgttcgccga ttccggctat acgggcgagg acgcaaccgg catggccgac gctatcggca
     1801 agcttttgaa tgcgcccgcg ctcaaggccg gaacagtgct gcgcgtcggc ctggaagttc
     1861 gcggcgaaac ggccaaggtc gtgcgcacca gcgtctatga caagacgcag cacatcgtca
     1921 ccatcgcgct cgacgatcat ggccagtacg tgccggccaa ggagccggaa gccaatcccg
     1981 aactgctcac cgcattcgac gggtcggcgc cggtaacggc gcgcggcaac ctgcccaata
     2041 tctatgacgg catctaccgc gcggcctatt cctatggaat gtcgaaaacc atgaccaagc
     2101 ggctggtcaa gcttttggcc tccgatgtcg atttccagtc gcgccttgcg ccgtcagacc
     2161 gcatcgaggt gcttttctcg cagcccgatg gcgacgacca ggcatccgac gattcggaac
     2221 tgctttatgt gtcgtcgacc ttcggcggcc agacccggac gctctaccgc ttccagatgc
     2281 aggatggcga cgccgactat ttcgatcagg acgggcgcag cgccgaacag ttcctgctgc
     2341 gcaaccccct gcccgccggg cgcttccgct ccgggttcgg cgcgcgcaag catccgatcc
     2401 tcggctacgt gcgcatgcat accggcgtcg attgggcagc gcccatcgga acgccgatca
     2461 tcgcggccgg caacggcgtc atcgagaagg tcggctgggc cggtggctat ggcaagcaga
     2521 tcatgatccg ccacgccaac ggctatgaaa cttcctacaa tcaccagagc gctttcgcca
     2581 atggcatcaa ggcgggcatg catgtccgcc aggggcaggt gatcggctat cttggccaga
     2641 ccggcctggc gaccggcccg catctgcact acgaattgat cgtcaacggc accaaggtgg
     2701 acccgatgcg ggttcgcctg ccggtcggca aggcgctgaa aggcgacgac ctcgtcgcgt
     2761 tcaagcgcga acgcgagcgc atcgacgact tgctgaagca ggaagatgcc gatccgctga
     2821 aggtcgccag cgccaaggtc gaaggctagg gcggttctgg tttttaccga atcggaaaaa
     2881 ccgctctatc tctttatttt ggccgcattt ccggacgcaa aaccgttcac gctttttgct
     2941 ggaaatactc taggctccgg atccgctctg gttgccgggt atcgtcatcc aggcgacgat
     3001 tgccgaaagc aggcaaagca cggccgtgac gagggcgacc gccgcgaaag ccgtatcgct
     3061 cgccgccatt cgcgccaggt ctaggctatg ggccatgccg gcttgcacgg gttcgccaaa
     3121 gcccggcgca tcggcgccac ccgccccgcc tagtgttttt gcatagacga cagccgccac
     3181 cgatcccatc gccgcgaccg cgataagccc gccaatgcgc gaaaccgcat tgtttatgcc
     3241 ggaggcggcc ccggtatcct tgtcctccac cgccgtcacg atcgcagtgg acagcggcga
     3301 tactaccagc gccatgccga ggcccagcag agccatcagc ggaaatatgc ctgtccagaa
     3361 atgatgaaag ccggcaaagg ccagcaacgc catgccggca aaggcgaaag caaccagcag
     3421 gctgccgacc gcaagcggca ggcgcgggcc tgccttgtcg gaaaaacgcc ctacccggcc
     3481 ggacagcagc gcgatgaagc ccgacaatgg caagaagatg aaaccgactt cgccggcgct
     3541 caacccccat ccggcgatga gcagcatcgg caggtagaac aggtttgccg acagggcgaa
     3601 gtatagaaag aaggtcgaca gattggcgcc cgcgaaggcc gggctgcgaa acaggccaag
     3661 gtcgatcatc ggctcgcgct gttttatctc ccagccgatg aaaacgacca gcaatacgat
     3721 gccggccgca atgctcattc cggaaaggcc gccctcgcct ttcccgttca gcgccgtcag
     3781 cccgtaggcg agcacgccga aggccagcgt ggcaagcgcg gcgccgccaa ggtcaagcgc
     3841 gcgcttttcg gtcggcttgt cggcgggaac cttggcgaaa agcagataga tcgaaaccgc
     3901 gccgagcggc aggttgatgg caaaaatcgc ccgccatatg ctttcgttga aggaggcgat
     3961 cagcagcccg cccagcaccg gacccagcgc cgtggtcaag gcggacgcgg ccgcccagat
     4021 gccaattgcc cgcccacgct cgttcttggg ataggccttg gcgatgatgg cgaggctgca
     4081 cggcaccatg atggcggcgc caattccctg gatggccctg aacagcacga gaacgggcgg
     4141 attgggagcc agcgcgcagg caagcgacgc caggatgaac agcccgatgc cggcgatgaa
     4201 cgtaccgcgc agaccgaaac ggtcaccggc ggctccgcct gcaaggatga gcgcggacag
     4261 cgtcagcgca taggcgttgg atatccactg cgcctgcgcg aggctggcgc cgagatcgac
     4321 gcggatggct ggaatggcga tcgccagcac cgagccgtcg atgaagccga gcgccgatgc
     4381 cagaatggcg gcgatcaaga caaagcggcg gctggattgc gggcagaacg aagctccgtt
     4441 cgccggacga gcctgcccgg ctgatgcttc gctcacttta tctatcgtct gcatggatcg
     4501 ccatcagcct ctcgccccgt catggtggcc gttgtcgagc gccaccgcaa gctggaattg
     4561 tcagctatcg accgtccatc gccagcccgt ccgggagcct tgaggaaatg tcttcttatg
     4621 cccgccaacc gtcgaacagc atcgacgggc ctgcgtcaag ctcccccagt tcgacccggt
     4681 ttcgcccttc gcgcttggca cgatagagcg atttgtcggc agccagcagc agcgcatcca
     4741 ggtcgacgat tggcttttcg gccatgacca ggccgatgct gacggttgcc gtgatgctgc
     4801 gtccatcgcc aacgtcgatc gtcgcctgct cgaacacggt tcgaatatgg tgcgcgatct
     4861 gctcggcgca ctcacgcgaa cagtcggaca gaagaatggc gaattcctcg ccgccgatcc
     4921 ggccaaacgg gtcgcccccg cgcagctttg cggcggcaag gcgggcaaag gcggacagca
     4981 cccggtcgcc ggccgcatgc ccataagtat cgttgatatc cttgaaccgg tcgatatcca
     5041 gcatcagcat ggcgacgggt tgttttgcgg ccgacggcaa tccgagaaga gcttgcgacc
     5101 gcgccttgaa cccgccgcga ttaagcagat cggtcagctg gtcgtgtgcg gccatgtgct
     5161 ggagacggcg catcaactcg ttacgcaccg ccgtgacgct cgccaccgtg accggagcca
     5221 aggcgatcag cgtcaccccc atgcggatcg acatcaccgt cgaccactca ttattgtcca
     5281 gcccaagcgg gatgtagccc atcgaaatcg caagcagcgt ccagccgctg aacagcagcg
     5341 tcagcaccgc cgtcatgaac tggctgtaga cgagcgcgca ccataacagg gcgggaaccg
     5401 gaaagccgat cgcaccggga ccgccgacga ccagaccggc aatggccgac aggacgagaa
     5461 aagccgccgg cgccagcata gccggcgtga cgttcaggct cgcgtaacga gaaacatccg
     5521 ccttgtcctt gctccatgcc agggcgggca aggtaaggat gaccggcagg attgcgatgt
     5581 agttgaccag ttcgctgacg aaccagaaac cccacccctc aaggacagac ccctggaaca
     5641 gcagcggatt ggcgatgccg ccgaccacac cggcggcggc cgcgcacagc gctgctccca
     5701 atacgagata gaaaatcgaa aggggacgtc caagccgctg gtcgagcggg tccagccgcg
     5761 aaaaaagctg atatccggcc atgacgctga ccatgttggc aattgtcagc agaagcgtca
     5821 tcaccagggg accgccgctg ataagatcgg cacacatgta agcaccggcg gcgccaagca
     5881 gcgcaaatgg ccgcgccagt ttcggattgc gcaccagcat gcccagcatc agggcgttcg
     5941 cgggccagaa cgccgacaga tgtccggccg ggcgcgtgaa attgccgaac aaggcggcgg
     6001 tgagaatgac gccggcgaaa atcgccacgc tcgtcaggct tgcggcgaac tggcccgaag
     6061 gctcttttcc ctgatcgagg ggcggcacat tttcgacgat aacccgcagc atttccgtgg
     6121 cccttacccc cgctggaacg caggctgtca tgctcggata aggcgcagga atttctcttt
     6181 taagcgttaa ccgtgcatgg ccgggtggcc cgccggaaag accgatctcc agtgatacaa
     6241 ggcttccaag ccagcattca atcgatgaat tccaccgtga cgccgggctt caccagcctg
     6301 gccagctcgc gcgcgtccca gttggtcagg cgcacgcagc catggctttc ggtcttgccg
     6361 atcttggagg gatcgggcgt gccgtgaatg ccgtaggtcg gtttgtcgag cgcgatccac
     6421 accgatccga ccgggccgtt cggccccgga ggaatggtca gcaccttgtc gttttcgcct
     6481 tgcttgaaat tgagcttcgg attgtaggta tagttgggat cgagcgcgat cctcgatacc
     6541 gcatgcgttc ccgaaggcga cggcgtgtcc gtcgaaccga tggtcgccgg ataagccgcg
     6601 accagcttgc cggccgcgtc ataggcgcga acctgcttca tgcctttgtc ggcgaggata
     6661 ttggtcacct gcgtcttggc cagcttaccg aaattggcaa ccttgatgac ggtgcccggc
     6721 cgattgaaat tcgcttccgg attgagcgcc ttgagatagt tctcgtccat atggaagcgt
     6781 tcggccagcg cttccgccac cgaggtgaag ctcatccggt cgagcttggc cttttcgctg
     6841 taatcgaggg gaaccgaggc cagatagggg cctgcggcat cttccggcgt gatcgtgtag
     6901 gaggcaaagg cgtcaccgcc cgaattcgcc agcgcctgcg tgatgccgat cgtgtcggtc
     6961 gtcttcaact gcgatccggt tatctgccgg taggcggcaa gcgccttgtc gacattggct
     7021 ccaaagcgcc cgtcgatgac gcccggcgaa gcgccggcgc ggtccatcaa cacctgcaac
     7081 gcggcaatgt cttcgcgttg gccaagcgac agggaagggt ctacccgcgt gcgcggcaca
     7141 tccgcgccgg caggctgttc cggctcgacc agaacttccg gctgcgcagg ctggatggag
     7201 gcctggtcca gcggcggcgc tgccttgcgg cgctcgacgt cgcgcggcgc ctgcggttcc
     7261 tcgggataag cctcgccata gccgccatcg tccgggacag ttccaaccga atcgtcgccg
     7321 cctggatcat attcgccgac ggacggagaa ccctcgccgc cctcttcttg aagccgcctg
     7381 cgccgaaacc gctccatgtc ttcgggatcg tccagataat agctgtcgtc ggccgaaccc
     7441 tggcgcaggt tgcgctggcg ctgcggcgcg cgcaaggcgc ggcgatcgaa cttcgtctgc
     7501 ggcggctgga tggcgacgac cttgcccgta taggcatcga cgatcacccg gttgcccttg
     7561 ccgtcgaagt aaacttcata atcgccattc tgggcgacga gcagtccgtt gccggctggc
     7621 gccttcacgg cctcgtcacg atgatcgtta gggcttggcg cggcaacgac catgcccggc
     7681 agcgaagcca gggcgaaggt cgagagcaac aaaatcgtgc ggagcatgac tgccaaactc
     7741 tttccgtatc ccgttcgccg ccatgcggcc ccgaaccgcc agcgaacctt tcgccagcaa
     7801 accatttcac aagatagggt tcttacatga accgggcatg aaaaaggcgg ctagcacggc
     7861 gttccgacag caacaaggcg ccgacgccat ggttttatcc cacggcaatc ggcgccccgt
     7921 catttttcag cccttcatta cgatagggca atccggccgg ctcaggcggc cacggcttcc
     7981 tcgataggcg tcggcttcga gcggaagttc agccggtcag aaccagcggt cacctttacc
     8041 gtcgagccat cgaggatatc gcccatcaag attttctcgg ccagcgggtc ttgcagttcc
     8101 ttctgcatca cccgctttag cggccgcgcg ccataggccg gatcgtaacc cttcgccgcc
     8161 agccattcga ccgcatccgc atcgagctga agcgcgatct tgcgatccg
//
LOCUS       DASYCS010000002         3418 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4059523,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000002 DASYCS010000000
VERSION     DASYCS010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4059523"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1656)
                     /gene="carB"
                     /locus_tag="VGM46_00035"
     CDS             complement(<1..1656)
                     /gene="carB"
                     /locus_tag="VGM46_00035"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006338245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEY4191008.1"
                     /translation="MPKRTDIKSILIIGAGPIVIGQASEFDYSGTQACKALREEGFRV
                     ILVNSNPATIMTDPELADATYIEPITPEVVAKIIARERPDALLPTMGGQTALNTALSL
                     RRMGVLDRYNVEMIGADAHAIDKAEDRSLFREAMAKIGLETPKSMLANASAVKDADRK
                     THEAERAALKAANPENLDAALDALETSWNLGEGDRKQRYIGHAMAIAAQAMDAVGLPA
                     IIRPSFTMGGTGGGIAYNRAEFYEIIQSGLDASPTTEVLIEESVLGWKEYEMEVVRDK
                     ADNCIIVCSIENIDPMGVHTGDSITVAPALTLTDKEYQMMRNASIAVLREIGVETGGS
                     NVQFAVNPADGRLVVIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYTLDELENDIT
                     GGATPASFEPSIDYVVTKIPRFAFEKFPGAETTLTTAMKSVGEVMAIGRTFQESLQKA
                     LRGLETGLTGLDEIEIPGIGPAGDDTDDRNAIRAALGTPTPDRLRMVAQAIRMGTSLE
                     DVHAMCKIDPWFLEQIAGILAMEERIREHGLPQDEANLRMVKAM"
     gene            complement(1832..2182)
                     /locus_tag="VGM46_00040"
     CDS             complement(1832..2182)
                     /locus_tag="VGM46_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006200050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY4191009.1"
                     /translation="MMLPHPFPEQILLPNVLAVLGDPIRLAIVRELARNAGEPMNCGR
                     FLDFASKTNVSYHLAKLREAGITHTEAVGTSRFTTLRRQELDARFPGLLDSVINAAVS
                     DGALPSLEQADAEA"
     gene            2376..>3418
                     /locus_tag="VGM46_00045"
     CDS             2376..>3418
                     /locus_tag="VGM46_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013530113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4191010.1"
                     /translation="MDKRLIWLAVGSFTISTEGFVISSLLPDIAADAHISVSLAGCLI
                     TAFALAYALGTPILATLTGEWDRRRVILSTMALFVLGNLGAAVSSSFELLLASRIAMA
                     LSSGLFAATAQATAVALVDPHHRARAIAVVVGGTTVAVAVGAPLGALIAAFAGWRGTF
                     FAIAGLGAIAGAILWFRLPRGIAGMRLPLTVRVAAALRPGVMPMLLTTLLAMTGPFVV
                     FSYIAPLAMEGTGLQEIALPALLLGFGVGAVIGNIAGGQAADRFGATRTVAWSMALIF
                     AMMILLALIPILLPHNLAGPALIAMMVPWGIAGWSFPPAQASRIVRLAPDAAPIVLSL
                     NGSALYLGVAFGAA"
BASE COUNT          515 a         1036 c         1168 g          699 t
ORIGIN      
        1 catggccttc accatgcgca gattggcctc gtcctgcggc aggccgtgct cgcggatgcg
       61 ctcctccatg gcgaggatgc cggcgatctg ttcgaggaac cacgggtcga tcttgcacat
      121 ggcgtgcacg tcttccagcg atgtgcccat gcggatcgcc tgcgccacca tgcgtagccg
      181 gtcgggcgtc ggcgtgccga gcgctgcgcg gatggcgttg cggtcgtcgg tgtcgtcacc
      241 ggcggggccg atgccgggga tctcgatctc gtcaaggccg gtgaggccgg tttccagccc
      301 gcgcaacgcc ttttgcaggc tttcctggaa ggtgcggccg atggccatga cttcgccgac
      361 cgacttcatc gcggtggtca gcgtcgtttc ggcgccgggg aatttttcaa aggcgaagcg
      421 cgggattttt gtcaccacgt agtcgatgga cggctcgaag gaagctggcg tcgcgccgcc
      481 ggtaatgtcg ttttccaact cgtccagcgt gtagccgacg gcaagcttgg cggcgatctt
      541 ggcgatgggg aagccggtgg ctttcgaggc aagggccgac gagcgcgaaa cgcgcgggtt
      601 catctcgatg accaccaggc ggccgtcggc cgggttgacg gcgaactgga cgttggagcc
      661 gccggtctcg acgccgatct cgcgcagcac cgcgatcgag gcgttgcgca tcatctggta
      721 ttccttgtcg gtcaaggtca gcgccggggc gacggtgatg ctgtcgccgg tgtgaacgcc
      781 catcgggtcg atgttttcaa tggagcagac gatgatgcaa ttgtcggcct tgtcgcgcac
      841 gacctccatt tcgtactctt tccagccgag cacgctttcc tcgatcagca cttcggtggt
      901 cggcgaggcg tcgaggccgg actggatgat ctcgtagaat tcggcgcggt tataggcgat
      961 gccgccgccg gtgccgccca tggtgaagga ggggcggatg atggcaggca ggccgacggc
     1021 atccatcgcc tgcgcggcga ttgccatggc gtggccgatg tagcgctgct tgcggtcgcc
     1081 ttcgccgagg ttccagctgg tttccagcgc atcgagggcg gcgtcgaggt tttccggatt
     1141 ggccgccttc agcgccgcgc gctcggcttc atgcgtcttg cgatcggcat ctttgacggc
     1201 cgaggcgttg gccagcatcg atttcggcgt ttcgagaccg atcttggcca tcgcctcgcg
     1261 aaacagggag cggtcctcgg ccttgtctat ggcgtgcgca tcggcgccga tcatctcgac
     1321 attgtagcgg tcgagcacgc ccatgcggcg cagcgacaaa gcggtgttga gcgcggtctg
     1381 gccgcccatg gtcggcagca gcgcatccgg gcgttcgcgc gcgatgatct tggccaccac
     1441 ttccggcgtg atcggctcga tataggtggc gtcggccaat tccggatcgg tcatgatggt
     1501 ggccgggttg gaattgacca ggatgacgcg gaaaccttcc tctcgcagcg ccttgcaggc
     1561 ctgcgtgccg gaatagtcga actcggaggc ttggccgatg acgatggggc cggccccgat
     1621 gatcaggatc gacttgatgt cggtgcgttt gggcatggcg atatccgttc gcaaggcatg
     1681 ttgcgcgaaa aaccgggacg ggaaacccgg ccggttgtgc tcaacgatta tgatgtcagg
     1741 ctaagtccgc cttataggga cagtttcgcg gttgcgtaac cccgaaaatg ccggaaaaaa
     1801 gcctatccac caggatagaa cggcagaagg ctcacgcttc ggcgtcggcc tgctccaggg
     1861 aaggcaaagc gccgtcgctg acggcggcat tgatcacgct gtcgagcagc ccgggaaagc
     1921 gggcatcgag ttcctgtcgt cgcaaggtgg tgaagcggct ggtgccgacc gcctcagtgt
     1981 gggtgatgcc tgcctcgcgc agcttggcca ggtgatagct gacattggtc ttggaggcga
     2041 aatccaggaa ccggccgcaa ttcatcggct cgccggcgtt gcgggcaagc tcgcgcacga
     2101 tggcgaggcg gatggggtcg ccgagaacag cgagcacatt gggcagcaag atctgttcgg
     2161 ggaaggggtg cggcaacatc atgctccaga attaagcgca gtctacgcgg atttcaatgc
     2221 gtctccttca agcgcgccgg cggcgcgcgc ctgcgcccac ggcatcgtgc tgacggagcg
     2281 ctgtcagcat ggctgcacta tttttcaacg ccgtcattga catataggca cgcgatgtcc
     2341 aattgttcaa cgatatttga actaaggata aaataatgga caagcgtctc atctggctgg
     2401 cggttggatc gttcacgatc tcgaccgaag gcttcgtcat ttccagcctg cttccggata
     2461 tcgcggccga tgcccatatt tcggtttcgc ttgcgggctg cctcattacc gcgttcgcgc
     2521 tggcctacgc gctcggcacc ccgatcctgg ccaccctgac cggggaatgg gaccggcggc
     2581 gcgtcatctt gtcgaccatg gcgctgttcg tgctcggcaa tctgggcgcg gcggtgagtt
     2641 cgtcgttcga gctgctgctc gcctcgcgca tcgccatggc gctttcgtcg ggcctgtttg
     2701 cggcaacggc gcaggcaacg gcggtggcgc tggtcgatcc gcaccatcgt gcgcgggcaa
     2761 tcgcggtggt cgtcggcggc accacagtcg cggttgcggt cggtgcgccg ctcggcgcgc
     2821 tgatagcggc tttcgccggc tggcgcggca ctttcttcgc catagccggt cttggcgcca
     2881 tcgccggcgc gatcctttgg ttccgcctgc cgcgcggcat cgccgggatg cgcctgccgc
     2941 ttactgtgcg ggtggcggcg gcgcttcgtc cgggcgtgat gccgatgctg ttgaccacgc
     3001 ttctggcgat gaccggacct ttcgtcgtgt tttcctacat cgcgccactg gcgatggagg
     3061 gaaccggact tcaggaaatc gcactgccgg ccctgctact cgggttcggc gttggcgccg
     3121 tcatcggcaa tatcgcaggc gggcaggcgg ccgaccgttt cggggccacg cgcaccgtgg
     3181 cctggtcgat ggcccttatc ttcgcgatga tgatcctgct ggcgctgatc ccgatccttt
     3241 tgccgcacaa cctggccggc ccggcgctga ttgcgatgat ggtgccgtgg ggcattgccg
     3301 gctggtcgtt cccgccggcg caggcaagcc ggatcgtcag gctggcgccc gacgcggccc
     3361 ccatcgtgct gtcgctgaac ggctcggcgc tttatctcgg cgtcgccttc ggcgcggc
//
LOCUS       DASYCS010000003        11341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1082, whole
            genome shotgun sequence.
ACCESSION   DASYCS010000003 DASYCS010000000
VERSION     DASYCS010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 11341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11341
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1082"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            309..857
                     /locus_tag="VGM46_00050"
     CDS             309..857
                     /locus_tag="VGM46_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY4191011.1"
                     /translation="MLRFALALFLLAVPSLAHATDAGWALLRDGGRVVLLNHAMTNGT
                     TDPANFDITKCTTQRNLSNRGKQQAGKIGALFGARSAPVERVLSSRYCRCLDTARIAF
                     EAEPEPFAPLDLLSTDEATKTKQLADIVGAVRDYSGSDNLVMVTHMENIRALTGVSPR
                     EGEAVVVGLDGDGLRALGRVTF"
     gene            1008..1289
                     /locus_tag="VGM46_00055"
     CDS             1008..1289
                     /locus_tag="VGM46_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEY4191012.1"
                     /translation="MSTTFEKVAKIIADTSEIDIDTITPESHTIDDLGIDSLDFLDIV
                     FAIDKEFGIKVPLEKWTQEVNDGKASTDEYFVMKNLCAKIDALIAAKNA"
     gene            1350..2552
                     /locus_tag="VGM46_00060"
     CDS             1350..2552
                     /locus_tag="VGM46_00060"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HEY4191013.1"
                     /translation="MNPRDVVITGIGLVSSLGEGPDVHWQKLTTPGLHPVTDSERFAP
                     YTVHPLPEIDWNMQIAKRGDQRQMETWQRLGTYAAGLALDDAGIKGDEALCTTMDMVV
                     AAGGGERDITVDSDILAASSARSDHDLLLNEKLTTELRPTLFLAQLSNLLAGNISIVH
                     KVTGSSRTFMGEEGAGVSAIETAAARIRSGQSTHMLVGGAFQTEHPDMLLGYELGGYL
                     HRGPWQPVWARRGSQGGGVVPGSGGAFLVLEQREHAEKRGGRIYAQLGPVVSDRARRS
                     NGELGATLTRLLEQTGLPDEAPLDEALLTISGASGGHAATKAEKSVLDIRAQVVARGF
                     STLTGHLKEAQFPFAVALAALAIHHKAAYPAFDPAEQPFSGVPEKILATAIGYHQFEG
                     MALVCAAD"
     gene            2565..3836
                     /locus_tag="VGM46_00065"
     CDS             2565..3836
                     /locus_tag="VGM46_00065"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018428326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HEY4191014.1"
                     /translation="MANLKDHMGRPVVAVTGIGVVTSLGAGKAANWAALTSGTSGIHP
                     ITRFPVDHLNTRISGMVDFLPSSTKGASALTYELAETAAREALTESGFETGDFGGPLF
                     LASPPVELDWHDRFSLYNSRSEGDTPDRLLAVARGMNALDMFDSAQFGTIADRLAELF
                     GTRGLPITLSTACASGATAIQLGVEAIRRGECDRALSIGADGSATVEALIRFSLLSAL
                     STNNDSPAKASRPFSKDRDGFVLAEGSAALVLESLESALARGATVLGILRGCGEKADD
                     FHRTRSKPDASPAIAAVKAALADAGLREDEIDYVNAHGTSTPENDKMEHLALSTVFGE
                     RIGAVPVSSNKSMIGHTLSAAGAVEAAFSLMTMREGVIPPTINYDNPDPAILLDVVPN
                     VSRKAKVGTVLSSSFGFGGQNTCLVMAREPV"
     gene            3992..5020
                     /locus_tag="VGM46_00070"
     CDS             3992..5020
                     /locus_tag="VGM46_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEY4191015.1"
                     /translation="MRALQLIEDRRLETVHLPPPPPPALGEVTLRIKAVALNHIDVWG
                     WRGMAFAKRKLPLVVGAEASGEVETVGPGVSNLLPGQLVSIYGARTCGLCRPCREGRD
                     NLCEHVSGVHGFHLDGFAQEKINLPARLLVPAPPGVDAIGAAVAPVTFGTVEHMLFDN
                     AKLQPGETILVHAGGSGIGSAAIQLAKKMGCTVITTVGSNEKFERAKALGADHVINYR
                     EDRFEGVTRKLTKKKGVDVVFEHVGADTFAGSMLCLKRGGRLVTCGSTSGVSSQINLM
                     QLFQQQLKLLGSFGCRMENMADAMQKMAAGLVSPVIDTEIGFDGIDSALKRMESRDVF
                     GKIILRVG"
     gene            5032..6009
                     /locus_tag="VGM46_00075"
     CDS             5032..6009
                     /locus_tag="VGM46_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006335415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HEY4191016.1"
                     /translation="MAQGPLSNFRRNFKFGFGLKLRKLNYWLVARVAMAAISVLRRLP
                     PDKALNFADRTARRIGPRVSRHRVALDNLRHAYPEKSNAEIEAIASDMWGNMAKLAAE
                     YIFLDQLFDYDPNAGTTGRVEVKGAENFQRIAGENKPHILFTGHLGNFELLPVAAATF
                     GMKITALFRPPNNPYLADYIQSTRSSTMGGLQPSSTGASFALANILEQGGNVGVLVDQ
                     KFSRGVETVFFNRLCQSNPVLGMLARHFDCDVYPARCIRLPGNRFRLEVEDRLELPRD
                     ASGSIDVAATVQLLANVVERWVREDPGQWMWFHKRWQISGRPRKLRQNA"
     gene            complement(6025..6951)
                     /locus_tag="VGM46_00080"
     CDS             complement(6025..6951)
                     /locus_tag="VGM46_00080"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DmsC/YnfH family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEY4191017.1"
                     /translation="MHPAPSIILFTTLSGLGYGLAAVLGLGLLDPAALATKTAHILAL
                     ALISGGLLSSTLHLGNPQRAWRALSQWRSSWLSREGVMAIVTFVPLLVCAWASIFENR
                     YLPVVGIVGTVCSIVTVYCTAMIYASLKSVQAWHTKLTPLCYRLFAVAGGFVAASLFA
                     FSGGGRNIALPLLAVVFLAAAWIAKMFWRKHLLETPPLSTPESATGLGGIGRVRLLER
                     PHVNENYLTSEMGFKVARKHAHKLWKIAVLLGGVLPGLLLLAVLLTGNKFGALAASLA
                     GLALVSHMLGMLTERWLFFAEARHAVMNYYGG"
     gene            complement(6956..7723)
                     /locus_tag="VGM46_00085"
     CDS             complement(6956..7723)
                     /locus_tag="VGM46_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018428330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEY4191018.1"
                     /translation="MTSLPSLPTPKKLGLVIDLDVCVGCHACVVNCKEWNTGGYGSAL
                     SDQDPYGADVSGAWLNRVHTFEVVPQGGEVVGEAGDARIVHFPKSCLHCDDAPCVTVC
                     PTGASYKRAEDGIVLVDEDMCIGCGLCAWACPYGAREMDLAAGVMKKCTLCIDRIYNE
                     TFEEVDRQPACVRTCPSNARHFGDFSDPHSAVSIMVLERGGMDLMPEQGTRPVNKYLP
                     PRARTPLATSARTSPLAENTDGAKGFFAWLDGMLERI"
     gene            complement(7890..10739)
                     /locus_tag="VGM46_00090"
     CDS             complement(7890..10739)
                     /locus_tag="VGM46_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HEY4191019.1"
                     /translation="MSPRPRVALSEPISDEVRKTTCYMCACRCGINVHIKDGKIRYIE
                     GNRDHPVNKGVLCAKGSAGIMQHYAPARLNAPLLRTGPRGSGEFKEISWDEALGIAAG
                     WLDTVRNDDPKKLAFFTGRDQSQSLTGFWAQQYGTPNYAAHGGFCSVNMAAAGIMTIG
                     GAFWEFGAPDWDRTKLFVMFGVAEDHDSNPIKMGIAKIKKRGARFVSVNPVRTGYSAV
                     ADNWIGIRPGTDGLLILSVVHELLKGRKIDVDYLVRYSNATWLVIDAPGTAEHGLFAR
                     DGQGRELVYETVTEQVVPAGAKGARPSLSGRVQLEDGRFAVPSFQLLADKYLDEKYAP
                     ESVAAETGVPAQTIRGLAAEIARVAFDEAIVIEQPWTDIHGVEHQSYVGRPVSFHAMR
                     GLSAHSNGFQTTRALHMLQVLIGAVDCPGGFRFEPPYPKPISAHPTPHGKAEHFGSNK
                     PLSGPHLGFPRGPEDLLIDAAGKPARLDKAFSWDAPIAAHGMMHMVIANAHAGDPYPV
                     DLMFLYMANMAWNSSMNTAGVIKMLEDKDEATGEYRIPKIIYSDAYASEMVAYADLVL
                     PDTTYLERHDCISMLDRPISEPDAVQDAIRWPVVEPDRDVRGFQTVLLDLGARLKLPG
                     FADNHGKALYKDYADYIVRHERRPGIGPLAGFRGLNGDKVGRGAANPEQLKRYIENGS
                     FFSAHIPMEAQFFKHANQAYQDFAVRMGFFDKPQPVTFQLYQETLQKFRLSALGLREP
                     FAPETHRQRIFEAFDPLPDWYEPLGEALVDHKAYPYHAITQRPAAMYHSWGSMNAWLR
                     QIHTKNPLYVPGRVCDELGLADGDWAWVISHHSRIKVEVARMEAVNSNTIWTWNAIGK
                     RKGAWGLDGDAPEAKKGFLMNHLIHELLPPKGDGLRWSNSDPVTGQAAWYDLRVRIEK
                     AEADETSEPQFSTIAQPGRAEPHPDELRYGQGWVK"
     gene            complement(10887..11318)
                     /locus_tag="VGM46_00095"
     CDS             complement(10887..11318)
                     /locus_tag="VGM46_00095"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEY4191020.1"
                     /translation="MKKTALVAAMLAMLSFGAIGESRAANLVARVDVSTQTMTVSKAG
                     QVLYTWHVSTARKGYFTPRGTYRPQRLHKMWYSRKYEMSPMPWSVFYHGGYAIHGTSA
                     VKRLGTPASHGCVRLQTANAARFSALVKEAGSANTKIIVAN"
BASE COUNT         2173 a         3676 c         3410 g         2082 t
ORIGIN      
        1 cgcatgaaat ggcttgtttt ctggagcatg tctttcaccc tgttctggtg cgctcaagcg
       61 cctgtttgca attcgtttga ccgggcggcc cggcaaaggc cgggcggccg aagtgccttc
      121 gttcataacg cacgggaagg cggacaggca atcgccgcat gccatgtggc cgctggtcca
      181 gcattgtccg gcggcgcaaa acggcgctcg cggcggactt gtctttcagc cggctttacc
      241 gtccctatat acgcgctgcc ggaatttgag gccaaccatg aattcaggcg agctacgaag
      301 gatgttccat gctgcgattt gccttggcgt tgtttttgct ggccgtaccc tcgctcgctc
      361 atgcgacgga tgccggctgg gcgctgctgc gcgacggcgg gcgcgttgtc ctgctcaacc
      421 acgccatgac caacggcacg acggatccag ccaatttcga catcaccaaa tgcacgacgc
      481 aacgcaatct ttccaaccgg ggaaagcagc aggccggcaa gataggcgcc ttgttcggcg
      541 cgcgctccgc cccggtcgag cgcgtcctct caagccgcta ctgccgttgc ctcgacaccg
      601 cccgcatcgc cttcgaagcc gaacctgaac ccttcgcgcc gctggacctg ttgtccacgg
      661 acgaagcgac aaagacgaaa caactggccg atatcgtcgg cgcggtgcgg gattattcag
      721 gctccgacaa tctggtcatg gtcacgcata tggaaaacat cagggccctg accggcgtgt
      781 cgccgcgcga gggcgaggcg gtggtcgtgg gcctggatgg cgacgggcta cgcgcccttg
      841 gccgggtcac tttctaggcg tcccaacaaa atagccgggg ttttcgccgc aattgccgct
      901 gcgggccggc ctgcgcggca cattcggtct tttcctgttt gaaaaaaacg actagacagc
      961 gcacgaacat atgcggacac aatccaccgc aatcgaagga aacgcccttg tccaccacat
     1021 tcgaaaaagt cgccaagatc attgccgaca ccagcgagat cgatatcgac acgatcaccc
     1081 cggaaagcca caccatcgac gacctcggca tcgacagcct cgacttcctc gacatcgtct
     1141 ttgccatcga caaggaattc ggcatcaagg tgccgcttga aaagtggacg caggaagtca
     1201 atgacggcaa ggcttccacc gacgaatact tcgtcatgaa gaacctttgc gccaagatcg
     1261 acgcgcttat tgccgcgaag aacgcctgac agccgcgcaa gccggctgtc gcccttgacg
     1321 ggcggcgttc cctgacccaa aaagcctcca tgaatccccg cgacgtcgtc atcaccggca
     1381 tcggccttgt ctcttcactg ggagaaggtc cggacgttca ttggcaaaag ctgaccacgc
     1441 cgggcttgca cccagtcacg gatagcgagc gctttgcccc ctacaccgtc cacccgcttc
     1501 cggaaatcga ctggaacatg cagatcgcca agcgtggcga ccagcgccag atggaaacat
     1561 ggcagcggct tggcacctat gcggccggcc ttgcgctgga cgacgccggc atcaagggcg
     1621 acgaagcgct gtgcaccacc atggacatgg tggtggcggc gggcggcggc gaacgcgata
     1681 tcacggtcga ttccgacatt ctcgccgcct cttcggcgcg cagcgatcat gaccttctgc
     1741 tgaacgagaa gctgacgacc gaactgcgcc ctaccctgtt cctggcccag ctttccaacc
     1801 tgcttgccgg caacatctcg attgtccaca aggtcaccgg ctcgtcgcgc accttcatgg
     1861 gcgaggaagg tgcgggcgtt tcagccatcg agacggccgc cgcgcgcatt cgttcgggcc
     1921 agtccacgca catgctggtc ggcggcgcct tccagaccga gcatcccgac atgctgctcg
     1981 gctacgaatt gggcggctat ctgcaccgtg gcccctggca gcccgtatgg gcaagacggg
     2041 gcagccaggg cggcggcgtc gtccccggtt cgggcggcgc attcctggtg ctggagcagc
     2101 gcgaacacgc cgaaaagcgc ggcggcagaa tttacgcgca gcttggtcct gtcgtttccg
     2161 accgcgcgag gcgcagcaac ggtgaactcg gcgcgaccct cacgcgcctg cttgagcaaa
     2221 cgggtttgcc ggacgaggct ccgcttgacg aggctttgct caccatttcc ggcgcgtcag
     2281 gcgggcatgc ggcgacgaag gccgagaaat cggtgctgga cattcgcgcg caagtcgtcg
     2341 cgcgtggctt ttcgaccttg actggacacc tgaaggaagc ccagttcccc ttcgccgtgg
     2401 cgcttgcagc gctcgccata caccacaagg ctgcctatcc ggctttcgat cccgccgagc
     2461 agccattttc gggagttccc gaaaaaatac tggcaaccgc gatcggctat caccaattcg
     2521 agggcatggc gctggtttgc gccgccgact gagagcgtga atacatggcc aatctgaagg
     2581 atcatatggg caggcccgtg gtcgccgtca ccggcatcgg cgtcgtgact tcgctcggcg
     2641 ccggcaaggc cgccaactgg gcggcgctga cttcgggaac gtccggcatc caccccatca
     2701 cgcggtttcc cgtcgaccac ctcaacaccc gcatctcagg catggtggat ttcctgccgt
     2761 ccagcacgaa aggcgcgagc gccctcacct acgagctggc cgaaacggcc gcacgcgagg
     2821 ccctcaccga atcgggcttt gaaacaggcg atttcggcgg cccgctgttc ctcgcctcgc
     2881 cgccggtgga actcgactgg cacgaccgct tttcgctcta caacagccgc agcgagggcg
     2941 acacgccgga tcgcctgctg gcggtcgcgc gcggcatgaa tgcgctcgac atgttcgaca
     3001 gcgcccagtt cggaaccatc gccgatcgcc ttgcagagtt gtttggcacg cgcggcctgc
     3061 cgatcacgct gtccaccgcc tgcgcctcgg gcgcgaccgc catccagctc ggcgtcgagg
     3121 ccatccgccg tggcgaatgc gaccgcgcgc tttcgatcgg cgcggatgga tcggccacag
     3181 tcgaggcgct gatccgcttc tcgttgcttt ccgcgctctc gaccaacaac gacagtccgg
     3241 cgaaggcttc gcggccgttc tcgaaagacc gcgacggctt cgtcctagct gaaggctcgg
     3301 cggcgctggt gctggaatcg ctggaaagcg ctttggcgcg gggcgcgacc gtgctgggga
     3361 ttttgcgcgg ttgtggcgaa aaggccgacg actttcaccg cacccgctcc aagcccgacg
     3421 cttcgcccgc gattgccgcc gtcaaggccg cccttgccga tgcgggcctg cgcgaagacg
     3481 agatcgacta cgtcaatgcg cacggcacat cgacgcccga aaacgacaag atggagcacc
     3541 ttgccctgtc gaccgtgttc ggcgagcgca tcggcgcggt tcccgtctcg tcgaacaagt
     3601 cgatgattgg ccacacattg tcggcggccg gggctgtcga ggccgcgttt tcgctgatga
     3661 ccatgcgcga aggcgtcatc ccgccaacca tcaactacga caatccggac cccgcgatcc
     3721 tgcttgacgt ggtgcccaat gtcagccgca aggccaaggt cggcaccgtg ctgtccagct
     3781 ccttcggctt cggcggccag aacacctgcc ttgtcatggc gcgggaaccc gtttaagcga
     3841 cccattcccg ctaaagcgtg tcgaaatctt ccagattcac ccgatacgcc ggcttttttg
     3901 tcttaaccca tgtctttttt taccttaacc catgtcctta tcccgaaatc gggaccgctt
     3961 tcggggacat gagtcaaacg gaaccaattc catgcgcgca ctgcaactta tcgaggatcg
     4021 ccggcttgaa acggtgcatt tgccgccgcc gccgccgccc gcgctcggcg aagtgacgct
     4081 gcgtatcaag gccgtggccc tcaaccacat cgacgtctgg ggctggcgcg gcatggcgtt
     4141 cgccaagcgc aagctgccgc tggtcgtcgg cgcggaagct tccggcgagg tcgaaaccgt
     4201 cggccccggc gtgtcgaacc tcctgcccgg ccagctcgtc tccatctatg gcgcgcgcac
     4261 atgcgggctg tgccgtccat gccgcgaagg gcgcgacaat ctgtgcgagc atgtgtcggg
     4321 cgtgcacggc tttcacctcg acggctttgc ccaggaaaag atcaacctgc cggcccgcct
     4381 tttggtgccg gccccgcccg gcgtcgatgc catcggcgcg gcggtcgcgc ccgtcacctt
     4441 cggcacggtc gagcacatgc tgttcgacaa tgcgaagttg cagcccggcg aaaccatcct
     4501 cgtccacgcc ggcggctccg gcatcggctc ggccgccatc cagctggcga aaaagatggg
     4561 ctgcaccgtc atcaccacgg tcggctctaa cgagaaattc gagcgcgcca aggcgctggg
     4621 cgccgatcat gtcatcaact accgcgagga ccgcttcgag ggcgtcacgc gcaagctgac
     4681 gaagaagaag ggcgtggacg tggttttcga gcatgtcggc gccgatacgt tcgccgggtc
     4741 catgctgtgc ctgaagcgcg gcggccggct cgtcacctgc ggctcgacct ccggcgtctc
     4801 gtcgcagatc aacctgatgc aattgttcca gcaacagctg aaactgctcg gctctttcgg
     4861 ttgccgcatg gaaaacatgg ccgacgccat gcagaagatg gcggcgggac tggtatcgcc
     4921 cgtgatcgac acagagatcg gcttcgacgg catcgatagc gcgctgaagc gcatggaaag
     4981 ccgcgacgtg ttcggcaaga tcatcctgcg cgtcggctga ggcgaccagc gttggcgcaa
     5041 ggccccctct cgaactttcg ccggaatttc aagttcggtt tcggcctgaa actgcgcaag
     5101 ctgaactact ggctggttgc gcgcgtggcg atggccgcca tttctgtgct gcgacgcctg
     5161 ccgcccgaca aggcgctgaa ctttgccgac cggaccgcgc gccgcatcgg cccgcgcgtc
     5221 agccgccacc gcgtcgcgct ggataatctg cgccacgcct atcctgaaaa gagcaacgcc
     5281 gagatcgagg ccattgcctc cgatatgtgg ggcaacatgg cgaagctggc cgccgaatat
     5341 atctttctcg accagctctt cgattacgat ccgaatgccg gcacaacggg ccgcgtcgag
     5401 gtcaagggtg cggaaaattt ccagcgcatc gccggcgaaa acaagccgca cattctgttt
     5461 accggccatc tcggcaattt cgagttgctg ccggtagcgg ctgcgacttt cggcatgaaa
     5521 atcaccgcgc tgtttcgccc gcccaacaac ccctatctgg ccgattatat ccagtcgacc
     5581 cgcagttcca ccatgggcgg ccttcagccc tcgtcgacgg gtgcttcctt tgcgctcgcc
     5641 aacattctgg aacagggcgg caatgtcggc gtgctggtcg atcagaaatt ctcgcgcggc
     5701 gtcgagacgg tatttttcaa ccgcctctgc cagagtaatc cggtgctggg gatgcttgcg
     5761 cgccacttcg attgcgatgt ctatcccgcg cgctgcataa ggctgcccgg aaaccgcttc
     5821 cgccttgaag tcgaagaccg actggaactg ccgcgcgatg cttccggcag catcgatgtg
     5881 gcggcgaccg ttcagcttct tgccaatgtc gtggaacgct gggtgcgcga ggaccccggc
     5941 cagtggatgt ggttccacaa gcgctggcaa atcagcggtc gcccgcgcaa actgcgccag
     6001 aacgcctgaa ttcaggcttg gccctcagcc gccataataa ttcatgacgg catggcgcgc
     6061 ttcggcgaag aacagccagc gttcggtcag catgccaagc atgtgggaaa ccagggcgag
     6121 gccggcaagg ctcgcggcca gcgcgccaaa tttgtttccc gtcaaaagaa cggccaacaa
     6181 aagcaagcca ggcaacacgc cgcccagcag caccgcgatc ttccaaagct tgtgtgcgtg
     6241 cttgcgcgcg accttgaaac ccatctcgct ggtcaggtaa ttttcgttga catgcggtct
     6301 ttccaacagc cgcacgcgcc caattccgcc aagaccggtg gcactttccg gcgtggaaag
     6361 cggcggcgtt tcaagcagat gtttgcgcca gaacattttc gctatccacg cggccgcgag
     6421 gaacaccacc gccagcaacg gcaacgcgat gttgcggccc ccgccgctga atgcaaacag
     6481 gctcgccgcc acaaagccgc cggcgaccgc aaacaaccgg tagcagagcg gcgtcagctt
     6541 cgtgtgccag gcctgcacgg atttaagcga agcatagatc atcgccgtgc aatagacggt
     6601 gacgatagag cagaccgtgc cgacgatgcc gaccaccggc aaataccggt tttcgaaaat
     6661 cgatgcccac gcgcagacaa gcagcggcac gaaagtgacg atcgccatca cgccttcgcg
     6721 cgacagccag cttgaacgcc attgcgacaa cgcccgccag gcgcgttggg gattgccgag
     6781 atgcagcgtc gacgacagca gcccgcctga aatcagcgcc agggccagga tatgcgcggt
     6841 cttggtggca agcgccgccg ggtcgagcaa gccaaggcca agcacggcgg ccagaccgta
     6901 gcccaggccg gaaagcgtgg tgaacaggat gatcgaaggt gctggatgca tttgttcaga
     6961 tacgctccag catgccgtcc agccaggcga aaaatccttt ggcgccgtcg gtgttttctg
     7021 caagcggcga ggttctggcg cttgtggcca gcggcgttcg cgcgcgcggc ggcagatatt
     7081 tgttgaccgg tcgcgtccct tgctccggca tcaaatccat gccgccgcgc tccagcacca
     7141 tgatcgacac ggctgaatgc gggtcggaaa agtcgccgaa atggcgggca ttggacgggc
     7201 aggtgcgcac gcaggcgggt tggcggtcca cctcctcgaa ggtttcgtta tagatgcggt
     7261 cgatgcacag cgtgcatttc ttcatgacgc cggcggcgag gtccatttcc cgcgcaccat
     7321 aggggcatgc ccacgcgcaa aggccgcaac cgatgcacat atcctcgtcc accagcacga
     7381 tgccgtcttc cgcccgcttg taggaagcgc cggtcgggca gacggtgacg cagggtgcgt
     7441 cgtcgcaatg caggcaggat ttggggaaat gcacgatgcg cgcatctccg gcctcgccaa
     7501 ccacttcgcc gccctgcggc accacctcga atgtgtgaac ccggttaagc catgccccgg
     7561 aaacatccgc cccatagggg tcctggtcgg aaagcgccga accatagccg ccggtattcc
     7621 attccttgca attgaccacg caggcgtggc agccgacgca gacatcgagg tcgatgacga
     7681 ggccgagttt tttgggtgtg ggtagcgagg ggagcgaagt cattggagcg caccccggca
     7741 caagaaagca gctcctcccc acaaggggga ggagaatctg ggaacgaccg aagccagcat
     7801 gggcgattgc gcgcaagcgc ggatacagac gttcctctcc ctcatgtgaa gaggacctag
     7861 gggtacaccg atagcggaat gcggcatcgt catttcaccc acccttgccc gtagcgcagt
     7921 tcgtcggggt gcggttccgc cctgcccggc tgggcaatcg tcgaaaattg cggctcgctc
     7981 gtctcgtccg cttcggcctt ttcaatgcgc acgcgcagat cgtaccacgc cgcctgcccc
     8041 gtcaccggat cggagttcga ccagcgcagc ccatcgcctt tgggcggtag aagctcgtgg
     8101 atcaagtggt tcatcaggaa gccttttttg gcttccggcg catcaccgtc cagcccccat
     8161 gcgcccttgc gcttgccgat ggcgttccac gtccatatcg tgttcgaatt gaccgcttcc
     8221 atgcgcgcca cctcgacctt gatgcggctg tgatgcgaga tcacccacgc ccagtcgcca
     8281 tcggccagcc ccagctcgtc gcagacccgc cccggtacat agagcgggtt tttagtatgg
     8341 atttgccgca accacgcatt catcgaaccc caggaatggt acatcgccgc cggccgctgc
     8401 gtgatggcgt gatagggata tgccttatgg tcgaccagcg cctcgcccag cggctcatac
     8461 cagtccggca acgggtcgaa cgcctcgaaa atgcgctgcc ggtgcgtttc gggcgcgaaa
     8521 ggctcgcgca atcccaacgc cgaaagccgg aacttttgca gcgtttcctg ataaagctgg
     8581 aacgtcaccg gctgcggctt gtcgaagaag cccatgcgca cggcaaaatc ctgataggct
     8641 tggttggcgt gcttgaagaa ctgcgcctcc atcgggatat gcgccgagaa gaacgacccg
     8701 ttttcgatat agcgcttcaa ttgctccgga ttggcggccc cgcgccccac tttgtcgccg
     8761 ttcaagccgc gaaagccggc cagcgggccg atgcccggcc ggcgttcatg gcgcacgata
     8821 tagtcggcat aatccttgta gagcgccttg ccgtgattgt cggcgaagcc cggcagcttc
     8881 aggcgcgcgc cgagatcgag caacaccgtc tgaaagccgc gcacgtcgcg gtccggttcg
     8941 accacgggcc agcgtatggc gtcctgtacc gcatccggct ccgaaatcgg cctgtcgagc
     9001 atcgatatgc agtcgtgccg ttcgagatag gtcgtgtccg gcaaaacgag gtcggcatag
     9061 gccaccatct ccgaggcata ggcatcggaa tagatgattt tcggaatgcg gtattcgccg
     9121 gtcgcctcat ccttgtcctc cagcatcttg atgacgccgg cggtgttcat cgacgaattc
     9181 cacgccatgt tggccatgta gaggaacatc aggtcgaccg gataaggatc gccggcatgg
     9241 gcgttggcga tcaccatatg catcatgccg tgcgcggcga tcggcgcatc ccaagaaaat
     9301 gccttgtcga gccgcgccgg cttgcctgcc gcgtcgatca gcagatcctc cggcccgcgc
     9361 ggaaagccca gatgcggacc ggacagcggc ttgttggagc cgaaatgctc ggccttgccg
     9421 tgcggtgtcg gatgcgccga aatgggcttc ggatagggtg gctcgaagcg gaagccgcca
     9481 gggcaatcga cagcgccgat cagcacttgc agcatgtgca acgcccgcgt cgtctgaaag
     9541 ccgttcgagt gggccgacag cccgcgcatg gcatggaacg ataccggccg cccgacatag
     9601 gattggtgct ccaccccatg aatatcggtc catggctgct cgatgacgat cgcctcgtcg
     9661 aacgccacgc gcgcgatttc ggcagccagc ccgcgaatgg tttgcgccgg cacgccggtt
     9721 tcggcggcga ccgattcagg cgcgtatttt tcgtccagat atttatcggc aagcaattgg
     9781 aaggacggca cggcaaaccg cccgtcctca agctgcaccc ggccggaaag cgaagggcgc
     9841 gcgcccttgg cgccggccgg caccacctgt tccgtcaccg tctcgtaaac cagttcgcgg
     9901 ccctggccat cgcgcgcaaa caggccgtgt tccgccgtgc ctggagcatc gatcaccagc
     9961 caggtcgcgt tcgagtagcg caccaggtaa tcgacatcga tcttgcggcc tttaagcagt
    10021 tcatggacga ccgagagaat cagaagcccg tcggtgccgg ggcggatacc gatccagtta
    10081 tccgccaccg ccgaatagcc cgtgcgcacg gggttgaccg acacgaagcg cgcgccgcgc
    10141 ttctttattt tggcgatgcc catcttgatc gggttggaat catggtcttc cgccacgccg
    10201 aacattacga aaagcttggt gcggtcccag tccggcgcgc cgaactccca gaacgcgccg
    10261 ccgatggtca tgatgccggc cgccgccatg ttgaccgagc agaagccgcc atgggccgcg
    10321 taattgggcg tgccatattg ctgcgcccag aaaccggtca gcgattgcga ttggtcccgt
    10381 ccggtgaaga aggcgagctt tttgggatcg tcgttgcgaa cggtatccag ccagccggca
    10441 gcgatgccca gcgcctcgtc ccacgagatt tccttgaact cgcccgaccc ccgcgggccg
    10501 gtccgcaaca acggcgcatt gagcctggcc ggcgcataat gctgcatgat gcccgccgaa
    10561 cccttggcac agagcacgcc cttattgacc ggatgatcgc ggttgccctc gatgtaacgg
    10621 attttgccat ctttgatatg cacgttgatg ccgcaacggc aggcgcacat gtagcacgtg
    10681 gtcttgcgaa cctcgtcgct aatcggctcg gatagcgcga cccttggtct gggactcatc
    10741 ggcacaactc ctggcgcatt gagtcctatc ggggcattgg cagaaaagaa atgccctgaa
    10801 tccgcatttc agggcaaact tcgtataagc aaacgtccat gcggagaacg aacatccgcc
    10861 gttgaaacgg cgcgtcggtt ctgaccctag ttggccacga ttattttcgt atttgccgaa
    10921 ccggcttcct tgaccagcgc agagaacctg gccgcattgg cggtctgaag ccgcacgcag
    10981 ccatgcgaag cgggcgtccc caaccgcttg accgcgctgg tgccgtgaat ggcatagccg
    11041 ccatggtaga ataccgacca tggcatcggc gacatttcat atttgcgcga ataccacatc
    11101 ttgtgcaggc gctgcggacg gtaggtgccc cgcggcgtaa aataaccctt tcgggccgtg
    11161 gatacgtgcc atgtataaag cacctggcca gccttgctga ccgtcatcgt ctgagtggaa
    11221 acatcgacgc gtgcgacaag attggcagcc cggctttcgc cgattgcacc aaaggaaagc
    11281 attgccagca tcgccgcgac aagcgctgtc ttcttcatcg gaccaaaccc ctctgaccgt
    11341 t
//
LOCUS       DASYCS010000004        14432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1305654,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000004 DASYCS010000000
VERSION     DASYCS010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 14432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14432
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1305654"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="VGM46_00100"
     CDS             complement(<1..419)
                     /locus_tag="VGM46_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008875511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY4191021.1"
                     /translation="MTAPHPEPKEAEPQGRIADNIVYFARALRKAGMRVGPASVKDAI
                     EAVLAAGIGSRDDFYWTLHAVLVSRHEDHATFDEAFRLYWKSRELIEKMLAMFSPVAP
                     DKRERIKPRAAENRVSQALMEGHEKDQPLREVPEIEV"
     gene            complement(423..1352)
                     /locus_tag="VGM46_00105"
     CDS             complement(423..1352)
                     /locus_tag="VGM46_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY4191022.1"
                     /translation="MTESKPRGVPQSIDETLDLLTGADYVADRSLATVLFLSLRMKRP
                     LFLEGEAGVGKTEIAKVLAQALGRRLIRLQCYEGLDVSSAVYEWNYAAQMIEIRMEEV
                     SGKVERSAMERNVFSEKYLIRRPVLDALTGEAGAAPVFLIDELDRTDEAFEAFLLEIL
                     SDFQVTVPELGTIKAQEPPIVIITTNRTREIHDALKRRCLYHWVDYPDAARELEIVRT
                     KVPQANSRLSAEVVSFVQKLRQIDLFKAPGVAETIDWAGALTELDKIALDPETVSDTI
                     GVLLKYQDDISRIGTGEGRRLLDEVKAELAAAE"
     gene            complement(1377..1871)
                     /locus_tag="VGM46_00110"
     CDS             complement(1377..1871)
                     /locus_tag="VGM46_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006332920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HEY4191023.1"
                     /translation="MLKKNDIGPQAYRDAMSLFAGHVHIVTTDGPAGKRGTTAIAACS
                     VSDTPPIILVCLNRENPRNDAFIENGRFALNTLTSHHRPLSVAFSGVTGLSAQKRFEL
                     GRWDTISTGAPTLADALVVFDCEMIDTKDLATHRVLFGKVTGLRTGDNLKPLIYHGRD
                     YHVL"
     gene            2046..3119
                     /locus_tag="VGM46_00115"
     CDS             2046..3119
                     /locus_tag="VGM46_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018427497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY4191024.1"
                     /translation="MHFHSITTMAVLAGFFLAGNAHAQAPQVGVAAPLSGPSEMLGRQ
                     VEAGASLAARMNGVQLTVADDGCSADGGASAAQKFVAAKVTVAVGFLCTKAIEAALPI
                     LKDAGIAVITVGVRTESLTDRRDKTGWPVFRLGPRGDGERNAIAAILTDLWRDELFAI
                     VDDGTIYGRELAETFRAAAEQAALKPVFTDTFRPGMDNQIGLIGRLKKAGATHVFVGG
                     DGSDMAIMGRDAEKLAPGMVFAGGEALRQPQDDAQYAAGTLMVALPEWAEIADKPTLD
                     AFAAANTIPEGYMLPAYAAVQVAVAASGLAETGKPVAQMLAGHDFATAIGPVRFDEKG
                     DLTQNPYRAFRFDGTRFVATGKK"
     gene            3119..4123
                     /locus_tag="VGM46_00120"
     CDS             3119..4123
                     /locus_tag="VGM46_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006206044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEY4191025.1"
                     /translation="MVRAGTKNLITDVAGLRVGNASDAPLKSGVTAILCDEPTIAGVQ
                     VLGGAPGTRETDLLEPHNTVEAVHAICLSGGSAFGLDAASGVQALLRERGIGFAVGDF
                     RVPIVPAAILFDLANGGDKDWGRYPPYRELGYEAALNASADFAIGTAGAGFGALSCGL
                     KGGLGSASAVLESGVTVGALVAANPVGSVTVGNTRHFWAAPFELDDEFGGLGYPSPMP
                     RDARDVSLKFRRDAMGGNTTIAVIATDAVLTKAAAKRLAMAAHDGFVRAVWPTHTPAD
                     GDLVFALATGKSGVVLSEIDAIDLYAEAGATMARAISRGVYAATAAEADVFPAWSSRR
                     "
     gene            complement(4131..4388)
                     /locus_tag="VGM46_00125"
     CDS             complement(4131..4388)
                     /locus_tag="VGM46_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006332917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2171 domain-containing protein"
                     /protein_id="HEY4191026.1"
                     /translation="MTDTSKIREHMEVIGADGVHVGTVDHIEGDRIKLTRRDSGQGSH
                     KGHHHYIPASLIAEIEGDKVRLSANSDVVANEFEEEQSDPT"
     gene            4483..4959
                     /locus_tag="VGM46_00130"
     CDS             4483..4959
                     /locus_tag="VGM46_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191027.1"
                     /translation="MRRAPPSLAATAIGAMLWAAAMAASALANLLLYQWETPAKIWTV
                     TLLFGLGGLAAFPAALFFARLATGRRGGETAFSAAFVALIVFTIVITSACFSLQHRLY
                     NAESHSLSFSPIWVLEFALTIVGALAQFVLLGTRLYFPIGFIALFIAGLWFARLPR"
     gene            5022..6329
                     /gene="purB"
                     /locus_tag="VGM46_00135"
     CDS             5022..6329
                     /gene="purB"
                     /locus_tag="VGM46_00135"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007770051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEY4191028.1"
                     /translation="MIPRYSRPEMVAIWSPETRFRIWFEIEAHACDALAELGVIPKEA
                     AQTIWEKGGAANFDVARIDEIEAVTKHDVIAFLTHLAEFVGPDSRFIHQGMTSSDVLD
                     TCLAVQLTRASDLLLADIDGLLAALKRRAFEHKDTITIGRSHGIHAEPTTFGVKLAEA
                     YAEFSRARERLVHAREDIATCAISGAVGTFANIDPHVEEHVAAKLGLKPEPVSTQVIP
                     RDRHAMFFVTLGVIASSIERLSIEIRHLQRTEVLEAEEYFAPGQKGSSAMPHKRNPVL
                     TENLTGLARMVRSYAMPAMENVALWHERDISHSSVERMIGPDATVTLDFALARMTSVI
                     DKLLVYPDNMLKNMNKFRGLVHSQRVLLALTQAGVSREDSYRLVQRNAMKVWEHGADF
                     LEELLADKEVTAALPEAEIREKFDLGYHTKHVDTIFRRVFGKS"
     gene            complement(6452..7267)
                     /locus_tag="VGM46_00140"
     CDS             complement(6452..7267)
                     /locus_tag="VGM46_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008875502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY4191029.1"
                     /translation="MSELPFAATTPVSVSRVGLKARNADALAAYYRAIVGLEELSRDG
                     ETITLGAKGRPLLVIESDPAAKQDDPRAAGLFHTAFLLPARADLGRWVNHAIAEKIQI
                     EGASDHIVSEAFYLTDPEGNGIEIYADRPHESWKWNGSQVAMATDRMDVPGVIGSVPA
                     GDAGWQGAPENTVVGHVHLRVGDPGAAEAWWHDQFGFDTAAKYGAQAVFLSSGGYHHH
                     IGANNWRSAGAGPRDPGLTGLSWVEMRSANAANASKHEDPWGTVIRTVPDKAI"
     gene            7517..8065
                     /locus_tag="VGM46_00145"
     CDS             7517..8065
                     /locus_tag="VGM46_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY4191030.1"
                     /translation="MKVKDADILIVPGYTNSGADHWQTRWEAKLSTARRVEQAEWSKP
                     VSEDWIANVVKAVNEAEKPVVIVAHSLGVPAVIQAVPQFTKPVAGAFLVAPPDVANPE
                     IRPKHLMTFGPYPREKLPFPSAVIASRNDPFCAFELAEGLAKDWGSLFIDAGEAGHIN
                     ADSGHGPWPEGSMTFAHFLTRL"
     gene            complement(8077..8856)
                     /locus_tag="VGM46_00150"
     CDS             complement(8077..8856)
                     /locus_tag="VGM46_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008875500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HEY4191031.1"
                     /translation="MSLKTRLAAGETLCTAWSGVPDPLTVEIVARQGFDAVTLDMQHG
                     GHHEDSVLRCIPSVHAVGKPVLVRIPVGRFDMASRALDFGTDAVIAPMVNSVDDAKAF
                     AASMKYPPLGQRSWGPTYAFPRHGKGTQAEWLHESNQRTLAFAMIETRAALDALDGIL
                     DVQGIDGIFVGPSDFSIAWSNGGTIDSTLEDMMETVRSIAERARKAGKFAATYIVDPA
                     ISGRMAKMGYSLLAMGSEHGLIALGAKNLLGAVRQSIDSAG"
     gene            complement(8981..9301)
                     /locus_tag="VGM46_00155"
     CDS             complement(8981..9301)
                     /locus_tag="VGM46_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1476 domain-containing protein"
                     /protein_id="HEY4191032.1"
                     /translation="MSNMKDREEGFERKFALDEEQRFRAMARRNKLLGLWAAEKLGKS
                     GADADAYAREVVNSDFEEAGDHDVFRKIRKDFDAASVDQSDHQIRRTMDELMAVAAEQ
                     IKNG"
     gene            9631..10425
                     /gene="purC"
                     /locus_tag="VGM46_00160"
     CDS             9631..10425
                     /gene="purC"
                     /locus_tag="VGM46_00160"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEY4191033.1"
                     /translation="MKNRRRIYEGKAKILYEGPEPGTLVQFFKDDATAFNAKKHEVID
                     GKGVLNNRISEHIFNHLNRMGIPTHFIRRLNMREQLIKEVEIIPLEIVVRNVAAGSLA
                     KRLGIEEGTVLPRSIIEFYYKADALDDPMVSEEHITAFGWASPQELDDIMALAIRVND
                     FLSGLFMGVGIQLVDFKIECGRLFEGDMMRIVVADEISPDSARLWDVATQDKMDKDRF
                     RRDMGGLVEAYQEVARRLGIMNENEPPRPAGPVLVASTDGPKGKPH"
     gene            10458..10700
                     /gene="purS"
                     /locus_tag="VGM46_00165"
     CDS             10458..10700
                     /gene="purS"
                     /locus_tag="VGM46_00165"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEY4191034.1"
                     /translation="MIKARVTVTLKNGVLDPQGKAIEHALSGLGFGGVGQVRQGKVFD
                     LELVATDKAKAEADLKAMCDKLLANTVIENYSVTLT"
     gene            10720..10983
                     /locus_tag="VGM46_00170"
     CDS             10720..10983
                     /locus_tag="VGM46_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191035.1"
                     /translation="MAEVDSSLIFETLKNIQGRLDKIDAGIGESRHEIVSLRLSQMSM
                     QNDIHNIYGVLARNDERLERIEHRLELRELAEKPQSPYEPPRA"
     gene            10980..11648
                     /gene="purQ"
                     /locus_tag="VGM46_00175"
     CDS             10980..11648
                     /gene="purQ"
                     /locus_tag="VGM46_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008875496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEY4191036.1"
                     /translation="MKSAVVLLPGLNRDRDLIAALTKISGTAPLTVWETDTEIPDVDL
                     IAIPGGFSYGDYLRCGAIAARMPVLRAVAEKAAKGVMVIGVCNGFQILVEAGLLPGAL
                     MRNASLKFACRQVKLQVANANTSFTNRYQPGQVISCPVAHHDGNYFADSETLARIEGE
                     GQVVFRYAQGTNPNGSINDIAGIVNSRGNVLGLMPHPENLIEAAHGGIDGRALFESAL
                     GIAA"
     gene            11680..12345
                     /locus_tag="VGM46_00180"
     CDS             11680..12345
                     /locus_tag="VGM46_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY4191037.1"
                     /translation="MKLYSRPLSPYSSIVRALAYLKDVPLKIIAPPAGFPIPEEFRAI
                     SPLNRIPVLITGSGETIVESVVIAEYLEERFPEPALLPADSKDRALVRMFARIVDLDV
                     LTPVTRLFELHSVPQRDQAAIDAQFKRLHYGLAAVDERMAQGPFALGDEVSFADAWLT
                     PTRFVFNHFREMTGSKDLLAAYPKFDAYQQIALQEPALSRVWGEMTDGLKIFLSELEA
                     GAA"
     gene            12342..12755
                     /locus_tag="VGM46_00185"
     CDS             12342..12755
                     /locus_tag="VGM46_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019857786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191038.1"
                     /translation="MIETSIARLAGLALAGLALASCQSGPKTPPAASGKSASLRAMEQ
                     VAIAAHRCWIASKDPAFKSYQMANELNNYGGTPRFLLVPAKQYGAKPLLVVQAQGASS
                     RVDAFGPLMTDPVGARIGSDIARWQSGDGSCGASA"
     gene            12757..13389
                     /locus_tag="VGM46_00190"
     CDS             12757..13389
                     /locus_tag="VGM46_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006206031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HEY4191039.1"
                     /translation="MGRALRMVGLALGLIGLFLQFCITVPASMEAGRSLFGSILFYFS
                     FFTILTNIGAVLVYVSLLSPSGYAWLPAFASPRMRAGVAVAIAVVFLVYITVLAPLQK
                     LEGASALCNILLHYGAPVIFVLWWLLAGRDGSSRWSDIALWLVYPLAYLAYALVQVPF
                     TGVVLYPFLDMAANGVAGVALSALTITAVFLVISVLAILADRHISRSSKA"
     gene            13435..14016
                     /locus_tag="VGM46_00195"
     CDS             13435..14016
                     /locus_tag="VGM46_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191040.1"
                     /translation="MKPDVYPKALPWLAGAVILFGIGLLLGWYYQFYPELRAETFDEN
                     GPVETLEMVALIAAAIIFAFRAVRSNDPIGTMCIPIVLILAIAFVRETPRCDSSFYDG
                     GLCFRTERWKDVTVTLFLIAAAIALFRRRQHLAAVLSIRKIHVFWPIWLSAVLLILAE
                     IGEAHGVLGTEESLEFFAYLYLASLGVWIHRNT"
     gene            14131..>14432
                     /locus_tag="VGM46_00200"
     CDS             14131..>14432
                     /locus_tag="VGM46_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010661202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase II"
                     /protein_id="HEY4191041.1"
                     /translation="MTISNTAPITAELIAAHGLKPDEYQRILDLVGREPSFTELGIFS
                     AMWNEHCSYKSSKKWLRTLPTTGAVVIQGPGENAGVVDIGDGDCVVFKMESHNHPS"
BASE COUNT         2624 a         4500 c         4478 g         2830 t
ORIGIN      
        1 tccacctcta tctccggcac ctcgcgcagg ggctggtcct tttcgtgacc ttccatcagc
       61 gcctggctga ctcggttttc ggcagcgcgc ggctttatcc gttcacgttt gtcgggagcg
      121 acgggcgaaa acatcgccag catcttctcg atcagctcgc gcgatttcca gtagagccgg
      181 aacgcctcgt cgaaggtggc gtggtcttca tgccgggaaa ccagcactgc gtgcagcgtc
      241 cagtagaaat cgtcgcgcga accgatgccg gcggccagca ccgcctcgat ggcgtccttg
      301 accgaagccg ggccgacccg catgccggcc ttgcgcagcg cacgcgcgaa atagacgatg
      361 ttgtcggcga tgcgtccttg cggctctgct tccttcggtt cgggatgcgg cgctgtcatg
      421 gcctactccg cagccgcaag ctcggccttg acctcgtcaa gaagccgccg accttcgcca
      481 gttccgatgc gggagatgtc atcctggtat ttcagcagca cgccgatagt gtcggaaacg
      541 gtttccgggt cgagcgcgat cttgtcgagt tcggtcagcg ctccggccca gtcgatggtt
      601 tcggcaacgc ccggcgcctt gaacaggtcg atctggcgca gtttctgcac aaaggaaacc
      661 acttccgccg aaagccggct gttggcctgc ggcactttgg tgcgcacgat ctccagttcg
      721 cgcgcggcat cgggataatc gacccagtgg taaaggcagc ggcgcttcag cgcgtcgtgg
      781 atttcacggg tgcggttggt ggtgatgatg acgatgggcg gctcttgcgc cttgatcgtc
      841 cccagttccg gcacggtcac ctggaaatcg gagaggattt ccagcaggaa cgcctcgaag
      901 gcttcgtcgg tgcggtcgag ttcgtcgatc aggaaaacgg gcgcggcgcc cgcctcgccg
      961 gtcagcgcat cgagcaccgg acggcggatc aggtattttt cggaaaagac attgcgttcc
     1021 atggcggagc gctccacctt gccggaaacc tcctccatgc ggatctcgat catctgcgcg
     1081 gcgtagttcc attcatagac ggcagatgaa acgtcgaggc cttcatagca ttgcaggcgg
     1141 atgagccggc ggccgagcgc ctgcgccaga accttggcga tctcggtttt gccgacgccc
     1201 gcctcgccct caagaaacaa gggccgcttc atgcgcagcg aaagaaacag cacggtcgcc
     1261 agcgaacggt cggcgacgta atccgcgccg gtcagcaggt cgagcgtttc atcgatggat
     1321 tgtggaacgc cgcgcggctt cgattcggtc atcgtatctc ccggccgtcg acgcattcac
     1381 aggacgtgat agtcccggcc atgatagatc agcggcttca aattatcccc ggtgcgcaag
     1441 cctgtcacct tgccaaaaag cacacgatgg gtggccaggt ccttggtgtc gatcatttca
     1501 caatcgaaaa ccaccagcgc gtcggcaagc gtcggcgcgc cggtcgatat ggtgtcccag
     1561 cggccaagct cgaagcgctt ttgcgccgac agaccggtaa cgccggaaaa ggcgacggaa
     1621 agcggacggt ggtgcgaagt cagcgtattc agcgcgaaac gcccgttttc gatgaaagcg
     1681 tcgttccttg gattttcacg gttgaggcag accaggataa tgggcggcgt gtcggacacg
     1741 gaacaggcgg cgatcgcggt cgtgccgcgc ttgccggctg gcccgtcggt ggtcacgata
     1801 tggacatgcc cggcaaacag gctcatcgcg tcgcgatagg cttgcggccc tatgtcattc
     1861 ttcttcagca ccggttattt cctgtcatct caaccctatc gctatatatg gccggatatc
     1921 gtgggccaca agcgaagtgc ggcgcgcgcc gcaaatgcca tgttgcgcgg gggccggcca
     1981 gcctttaggt cttggcatga gacatcggcg catggatgcg ccgcaaagag ggttcctgcg
     2041 cccgaatgca ttttcattcg atcacgacga tggcggtact ggcggggttt ttcctggcgg
     2101 gcaacgcaca tgcgcaggca cctcaggtcg gggtagcggc cccgctttca gggccatccg
     2161 aaatgcttgg caggcaggtc gaggcgggcg cttcgcttgc cgcgagaatg aatggcgttc
     2221 aattgaccgt cgccgacgat ggatgttcgg ccgatggcgg ggcgagtgcc gcgcaaaaat
     2281 tcgtcgcggc gaaagtcacc gtcgcggtgg gctttctttg caccaaggcc atcgaggcgg
     2341 cgctgcctat cctgaaggat gcgggcattg ccgtcatcac ggtcggcgtg cggaccgaaa
     2401 gcctgaccga tcgccgcgac aagaccggct ggccggtgtt ccggcttggg ccgcgcggcg
     2461 acggcgagcg caatgccatt gccgcgatcc tcaccgatct gtggcgcgac gaattgttcg
     2521 ccatcgtcga cgacggcacg atctatggcc gcgaattggc cgaaacgttt cgcgccgccg
     2581 ccgaacaggc ggcgctgaag ccggtgttca ccgacacatt ccggcccggc atggacaacc
     2641 agatcgggct gatcggacgg ctgaagaaag ccggcgcgac gcatgtgttc gtcggcggcg
     2701 acggttccga catggccatc atgggccgcg atgccgaaaa gctcgcgccg ggcatggtgt
     2761 ttgccggcgg cgaagctttg cgccagccgc aggacgacgc gcaatatgcg gccggcacgc
     2821 tgatggttgc cttgcccgaa tgggccgaga ttgccgacaa accgacgctc gatgcgtttg
     2881 ccgccgcaaa caccattccc gaaggctaca tgctgccggc ctatgcggcg gtgcaggtgg
     2941 cggtggcggc aagcggactg gccgaaaccg ggaaaccggt ggcgcaaatg cttgccggcc
     3001 acgacttcgc caccgccatc ggcccggtgc gcttcgacga aaaaggcgat ctgacgcaaa
     3061 acccctaccg cgccttccgt ttcgacggga cgcgcttcgt cgcaacgggt aagaaatgat
     3121 ggttcgcgcc ggcacgaaaa acctgattac cgatgttgcg ggactgcggg tcggcaacgc
     3181 ttctgatgcg ccactgaaat ccggcgtcac cgcgatcctt tgcgatgagc cgacgattgc
     3241 cggggtgcaa gtgcttggcg gcgcgcccgg cacgcgcgaa accgaccttc tggagccgca
     3301 caacaccgtg gaagccgtac atgcgatctg cctgtcgggc ggctccgcct tcgggctgga
     3361 cgcggcgtcg ggcgtgcagg cgttgctgcg ggagcgcggc atcggttttg cggtcggtga
     3421 ttttcgcgtg cctatcgtgc cggcggcgat cctgttcgac cttgccaatg gcggcgacaa
     3481 ggattggggg cgctatccgc cctatcgcga actgggctac gaggccgcgc tgaacgcttc
     3541 cgccgatttt gccatcggca ccgccggcgc cggctttggc gcgctttcgt gcgggctgaa
     3601 aggcgggcta ggctccgcat cggcggtgct ggaaagcggc gtcaccgtcg gcgcgctggt
     3661 cgccgccaac ccggtcggct cagtcacggt aggcaacaca cgccatttct gggccgcgcc
     3721 attcgaattg gacgacgagt tcggcggtct gggatacccc tcgccaatgc cgcgcgacgc
     3781 gcgcgacgtt tcgttgaaat tccggcggga cgccatgggc ggcaacacaa ccatcgccgt
     3841 aatcgccacc gatgcagtgc tgaccaaggc ggcggccaag cggctggcga tggctgcgca
     3901 tgacggcttc gttcgcgccg tctggccgac gcatacgcca gccgacggcg acctcgtttt
     3961 cgcgctggcg acgggaaaaa gtggcgtcgt gctctcggag atcgacgcca tcgacctcta
     4021 tgcggaggca ggcgcgacca tggcgcgcgc catcagccgc ggcgtttatg ccgccacggc
     4081 agcagaggcg gatgtatttc cggcgtggtc gtcacgccgt tgacgcgcct ttacgtcggg
     4141 tcggactgtt cttcctcgaa ctcgttggcc acgacatcgg aatttgccga gaggcgcacc
     4201 ttgtcgcctt cgatttcggc gataaggctg gcgggaatat aatgatgatg gcccttgtgg
     4261 gagccttggc cgctgtctct ccgggtaagc ttgatcctgt cgccctcgat gtgatccacg
     4321 gtgccgacat gaacgccatc ggcgccgata acttccatat gttccctgat cttcgacgta
     4381 tcggtcatga tggttctccg ttggaatcgt ggcacaacgg gccaaaccgc gattggatgc
     4441 atcgaaatcg gacaagagaa cgtgaatagc gcgtcgggcc gcatgcgccg tgcaccgcct
     4501 tccctggcgg cgacggccat cggggcgatg ttgtgggcgg cggcgatggc agccagcgcg
     4561 cttgccaacc ttcttctcta ccaatgggag acgccggcca aaatctggac ggtaacgctt
     4621 ctgtttggcc tgggtggcct ggcggcgttt ccggccgcgc tgttttttgc ccggttggcg
     4681 accggcaggc gcggcggcga aaccgccttt tcggctgcgt tcgtggcgct catcgtcttt
     4741 accatcgtca tcaccagcgc gtgtttctcg ctgcaacacc gcctttataa tgcggaatcg
     4801 cattcgctgt cgttttcacc gatctgggta ttggagttcg ccttgacgat tgtcggcgcg
     4861 cttgcccagt tcgtcttatt gggcacgcgg ctttatttcc cgatcggctt catcgcgctg
     4921 ttcatcgctg gcctctggtt tgcgcgcctg ccgcgttgag cattttgcca ccctctgata
     4981 ggaggcaggc caagactcgc cgccctgaag gaacctcccg catgatccct cgctattccc
     5041 gcccggaaat ggtcgccatc tggtcgccgg aaacgcgttt ccgcatctgg ttcgagatcg
     5101 aggcgcatgc ctgcgacgcg ctggccgagc ttggcgtcat ccccaaggaa gcagcccaaa
     5161 cgatctggga aaaaggcggc gcggccaatt tcgacgtcgc gcgcatcgac gagatcgagg
     5221 ccgtcaccaa gcatgatgtg atcgcgttcc tgacccatct ggcggaattc gtcgggccgg
     5281 attcgcgctt catccatcag ggcatgacct cgtccgatgt gctggatacc tgccttgccg
     5341 tgcagcttac ccgcgccagc gacctgttgc tggccgacat tgacgggctg ctggccgcgc
     5401 tcaagcggcg cgccttcgag cacaaggaca cgatcaccat cggccgcagc catggcatcc
     5461 acgccgagcc gacgaccttc ggcgtcaagc tggcggaagc ctatgccgag ttttcccgcg
     5521 cccgcgagcg gctggtgcat gcgcgcgaag acatcgccac ctgcgccatt tccggcgcgg
     5581 tcggcacgtt cgccaacatc gacccccatg tggaagaaca cgtcgcggca aagctcgggc
     5641 tgaagccgga accggtatcg acgcaggtta ttccgcgcga ccgccacgcg atgtttttcg
     5701 tcacgctcgg cgtcatcgcc tcgtccatcg agcggctttc catcgaaatc cgccatttgc
     5761 agcgcaccga agtgctggag gccgaggaat atttcgcgcc cggccaaaaa ggctcgtcgg
     5821 cgatgccgca caagcgcaac ccggtgctga ccgaaaacct gaccggcctc gcccgcatgg
     5881 tgcgttccta cgcgatgccg gccatggaaa acgtggcgct atggcacgaa cgggacatct
     5941 cgcattcgtc ggtggagcgc atgatcgggc cggacgccac ggtaacgctc gatttcgcgc
     6001 tggcgcggat gaccagcgtc attgacaagc tgctggtcta cccggacaac atgctgaaga
     6061 acatgaacaa attccgcggc ctggtgcatt cgcagcgtgt gctgctggcg ctgacgcagg
     6121 ctggcgtttc gcgcgaggat tcctaccgtc tggtgcagcg caacgccatg aaggtgtggg
     6181 agcacggcgc tgatttcctt gaggaattgc tggccgacaa ggaagtgacc gccgccctgc
     6241 ccgaggccga aatccgcgag aaattcgatc tcggctatca caccaagcat gtcgatacga
     6301 ttttcaggcg cgtattcggg aagagttgag catctggccg gcgcggtatg ccctgcgtga
     6361 cgtcatcccc ggctcgatac cgtcatcctc gggcttgtac cgaggatgac ggcgcggggg
     6421 ctggcaactc tctacaaaag cagctcgcgc ctcatattgc cttgtccggc accgtgcgga
     6481 tgaccgtgcc ccacgggtcc tcgtgcttcg aggcgtttgc tgcattggcc gagcgcatct
     6541 cgacccagga caggccggtg aggcctggat cgcgagggcc ggcgccggcg ctgcgccaat
     6601 tattggcgcc gatgtggtgg tgatagccgc ccgacgacag gaaaaccgcc tgcgcgccgt
     6661 atttcgccgc cgtatcgaag ccgaactggt cgtgccacca ggcttccgcc gcgccaggat
     6721 cgccgacgcg caggtggaca tggccgacga ccgtgttttc cggcgcgccc tgccagccgg
     6781 catcgccggc gggaaccgaa ccgatgacgc ccggcacgtc catgcgatcc gtggccatgg
     6841 cgacctgcga accgttccac ttccagctct catgcgggcg gtcggcataa atctcgatgc
     6901 cgttaccttc gggatcggtc aaataaaacg cctcgctgac gatatggtcg gaagcgccct
     6961 cgatctggat cttttcggcg atggcatggt tgacccagcg gccgaggtcg gcccgcgcgg
     7021 gcagcaggaa agcggtgtgg aaaaggccgg cggcgcgtgg atcgtcctgc ttggctgccg
     7081 gatcgctctc gatcaccagc aaggggcggc ctttcgcgcc caaggtgatc gtctcgccat
     7141 cgcgggaaag ctcttccagc ccgacaatgg cgcggtaata ggcggcaagc gcgtcggcat
     7201 tgcgggcctt gagcccgacg cgggaaacgc tgaccggggt ggtggcggca aagggcagtt
     7261 cgctcatctg aaagctccga tcagcctgcc ttcccccact ccccgtgaaa gaaggcccgg
     7321 catagtcaac gattatgcgc cgcatcaaaa aggccgatcc ttttcgcgac ggcacaggct
     7381 tgtttccacc ccaagatcgg cgattgccat cgttcataca agtgaggaaa aagcgaacat
     7441 ggtgttcaca gttgcagcgg gatagccgaa tttttgcccc gttgcgcgaa ggcttcgcgc
     7501 tcgctacatg cgcgtcatga aagttaaaga cgcagatatc ctcatcgtgc cgggctacac
     7561 caattccggc gccgaccact ggcagacccg ctgggaggcc aagctttcga ccgcgcggcg
     7621 cgtcgagcag gccgaatggt cgaagccggt cagcgaagac tggatcgcca atgtggtcaa
     7681 ggcggtgaac gaggcggaaa agccggtggt catcgttgcg cattcgcttg gcgtgccggc
     7741 ggtcatccag gctgtgccgc aatttaccaa gccggtggcc ggcgccttcc tggtggcgcc
     7801 gcccgatgtc gccaatcctg agatccggcc gaagcacttg atgaccttcg gcccctaccc
     7861 gcgtgaaaag ctgccgtttc cctcagcggt gatcgccagc cgcaacgacc ctttctgcgc
     7921 cttcgaacta gcggaaggcc tggccaagga ctggggttcg ctgttcatcg acgcgggcga
     7981 agccggccat atcaatgccg attccggcca tggtccctgg ccggaaggct ccatgacctt
     8041 cgcgcacttc ctgacgcggc tctagaattt cgacgcttag cctgcggaat cgatcgattg
     8101 cctgacggcg ccaagcaggt ttttcgcgcc cagtgcgatc aggccgtgct cggaacccat
     8161 ggccagcagg ctatatccca tcttggccat acggcctgaa atggcggggt cgacaatata
     8221 ggtcgccgcg aacttgccgg ccttgcgcgc gcgctcggca atcgagcgca ccgtctccat
     8281 catgtcttcc agcgtcgaat cgatggtgcc gccattgctc cacgcaatgg aaaaatcgga
     8341 cggaccaaca aagatgccgt cgatgccctg cacgtcgaga atcccgtcca gcgcatcgag
     8401 cgcggcgcgc gtttcgatca tggcgaaggc cagcgtgcgc tggttgcttt catgcagcca
     8461 ttcggcctgc gtgcccttgc catggcgcgg aaaggcgtag gtcggccccc acgaacgctg
     8521 gccgagcggc ggatatttca tcgaggccgc gaaggccttg gcgtcgtcca ccgaattgac
     8581 catcggcgca atgacggcgt cggtgccgaa gtcgagcgcg cggctggcca tgtcgaagcg
     8641 cccgaccgga atgcgcacca gcaccggctt tccaaccgca tggaccgatg ggatgcaccg
     8701 cagcacgcta tcctcgtgat ggccgccatg ctgcatgtcg agggttacgg catcgaagcc
     8761 ctgcctggcg acgatttcga ccgtcagcgg gtcgggaacg ccggaccacg ccgtacacag
     8821 ggtttcaccg gccgccagcc ttgttttcag ggacatcgca cttcctcctc catcgattcg
     8881 agtttcaccc ggaaacgact ggaaaagcaa agaaaaaccg cccgcacaga gcgggcgggc
     8941 gttgatccag tcagattgac ccagtcaggc ggaaggctgc ctagccgttc ttgatctgct
     9001 cggcggcgac ggccatcaat tcgtccatgg tgcggcggat ttgatggtcg gactgatcga
     9061 cgctcgccgc atcgaaatcc ttgcgtatct tgcgaaacac gtcgtggtcg cccgcttcct
     9121 cgaagtcgga attgacgact tccctggcat aggcgtcggc atcggcgccg ctcttgccca
     9181 gcttctcagc ggcccacagg ccgagcagct tgttgcggcg agccattgcg cggaatctct
     9241 gctcctcatc cagcgcgaac ttgcgctcga aaccttcctc acggtccttc atgttgctca
     9301 ttgcgggctc tccaggtgaa aactgcgatt cggttgcgtt cagggatatg tgtatggatt
     9361 gcacaaacca aaagggcgtc gatcggtcaa tatgttccgt ttgtcgcaag ctgcgacgtt
     9421 tggatgttcc gtctgtcgca agccgcgacc tttggatgtt ccattggccg caagccgcga
     9481 cctttggcgt tggaaagcgg ggattgggcg gatttgccga ttgagcaaac gatgcgtttg
     9541 cgatagaccg cgagtggaaa tactctgccg cagccatatt tcatgccgga tgccatgcat
     9601 ttggcgtcat ccagacttca gagacaatag atgaaaaatc gccgccgcat ttatgaaggc
     9661 aaggccaaga tcctctatga aggaccggag ccgggtacgc ttgtgcagtt cttcaaggac
     9721 gacgcgacgg ctttcaacgc caagaaacat gaagtcatcg acggcaaggg cgtgctcaac
     9781 aaccgcattt ccgagcacat cttcaaccac ctcaaccgga tgggcattcc gacccacttc
     9841 atccgccggc tcaacatgcg cgagcagctg atcaaggaag tcgagatcat tccgcttgag
     9901 atcgtggtgc gcaacgtcgc ggccggctcg ctggccaagc gcctgggcat cgaggaaggc
     9961 accgtgctgc cgcgctcgat catcgagttc tactacaagg cggacgcgct ggacgatccg
    10021 atggtctcgg aagagcacat caccgccttt ggctgggcct cgccgcagga gctggacgac
    10081 atcatggcgc tggccatccg cgtcaacgat ttcctgtccg ggctgttcat gggcgtcggc
    10141 atccagctcg tcgatttcaa gatcgaatgc ggccgcctgt tcgagggcga catgatgcgc
    10201 atcgtggtgg ccgacgagat ttcgcccgac agcgcccgcc tgtgggacgt ggcgacgcag
    10261 gacaagatgg acaaggaccg tttccgccgc gacatgggcg ggctggtcga agcctatcaa
    10321 gaagtcgcgc gccgcctcgg catcatgaac gaaaacgagc cgccgcgccc cgccgggccg
    10381 gtgctggttg cttccaccga tggcccgaag ggcaagcctc actgatcttt tccgccatag
    10441 caggagcacc gcctaaagtg attaaagccc gcgtcaccgt taccttgaag aacggcgtgc
    10501 tagacccgca aggcaaggcg atcgaacatg ccctgtccgg cctcggtttc ggcggcgtcg
    10561 gacaggtccg ccagggcaag gtgttcgacc tcgaactggt cgcgaccgac aaggccaagg
    10621 ccgaggcgga cctcaaggcg atgtgcgaca agctgctggc caatacggtg attgagaatt
    10681 atagtgtaac gcttacctga ggttgtgccg gagggcggag tggccgaagt cgatagctcg
    10741 ctgatattcg aaactctgaa gaatatacaa ggccgattgg acaagatcga tgcagggatc
    10801 ggagagagcc ggcatgagat cgtttccttg cggcttagcc agatgagcat gcaaaacgac
    10861 atccacaaca tttacggtgt gcttgcacgc aatgatgagc gtctggagcg tatagagcac
    10921 cgcctcgaac tgcgcgaact ggccgagaaa ccacaaagcc catacgagcc ccctagagca
    10981 tgaaatcagc cgtcgtcctc cttcccggcc tcaaccgcga ccgcgacctg atcgcggcgc
    11041 tgaccaagat ttccggcacg gcgccgttga ccgtctggga aaccgacacc gaaatccccg
    11101 atgtcgacct gatcgccatt cccggcggct tttcctacgg cgattatctg cgctgcggcg
    11161 cgatagcggc gcgcatgccc gtgctgcggg cggttgccga aaaggcggcc aagggcgtca
    11221 tggtgatcgg ggtttgcaac ggcttccaga tattggtcga ggcagggctt ttgcccggcg
    11281 cgctgatgcg caacgcctca ttgaaattcg cctgccggca ggtcaagcta caggtcgcca
    11341 acgccaacac gtctttcacc aaccgctacc agcccggcca ggtgatttcc tgccccgtcg
    11401 cccatcatga cggcaattat ttcgccgatt ccgagacgct tgcccgcatc gagggcgaag
    11461 gccaggtggt gttccgttat gcgcagggca ccaaccccaa cggttcgatc aacgatatcg
    11521 ccggcatcgt caattcgcgc ggcaacgtgc ttggcctgat gccgcacccg gaaaacctga
    11581 tcgaggccgc ccatggcggc attgacgggc gcgccctgtt cgaaagcgca ctgggcatcg
    11641 ccgcttgaac cccacaaccc cctacggaga gcaacggtca tgaagcttta ttcgaggccg
    11701 ctgtcgccct actcctccat cgtgcgcgcg ctggcctatc tgaaggatgt gccgctcaag
    11761 atcatcgcgc cgcccgccgg cttccctatc ccggaagaat tccgcgccat ttcgccgctc
    11821 aaccgcattc cggtgctgat tacggggtcg ggcgaaacca tcgtggaatc ggtggtgatc
    11881 gcggaatatc tggaagaacg ctttcccgaa cctgcgctgt tgccggcgga ctcaaaggac
    11941 cgcgcgctgg tgcgcatgtt cgcgcgcatc gtcgatctgg acgtgctgac gccggtgacg
    12001 cggctgttcg aactgcattc tgtgccgcag cgcgaccagg cagccatcga cgcgcagttc
    12061 aagcgtctgc attacgggct ggccgccgtc gatgagcgta tggcgcaagg gccgttcgcg
    12121 ttgggcgacg aggtaagctt cgccgacgcc tggctgacgc cgacgcgctt cgtcttcaat
    12181 catttccgcg agatgaccgg cagcaaggac ctgcttgccg cctatccgaa gttcgatgcc
    12241 tatcagcaga tcgcgctaca ggaaccggcg ctgtcgcgcg tctggggcga gatgaccgac
    12301 ggattgaaaa tattcctttc cgaacttgaa gccggggccg catgatagag acctcgatcg
    12361 cccgccttgc cgggctggct ctggccggcc ttgcgctggc ctcctgccag tccggcccga
    12421 agacgccgcc cgccgcctcg ggcaaaagcg catcgctgcg cgccatggaa caggtggcga
    12481 ttgccgcgca cagatgctgg atcgccagca aggacccggc cttcaagtcc taccagatgg
    12541 ccaatgagct gaacaattac ggcggcacgc cgcgtttcct gctggtgccg gccaagcaat
    12601 atggcgccaa gccgctgctg gtggtgcagg cgcagggcgc ttccagccgc gtcgacgcct
    12661 tcggcccgct gatgaccgat cctgtcggtg cgcgcatcgg ctccgacatc gcccgctggc
    12721 aaagcggcga cggcagttgc ggtgcaagcg cctaggatgg ggcgcgcgct gcggatggtc
    12781 ggactggccc tgggcttgat cgggctgttc ttgcagttct gcatcaccgt tcccgcctcg
    12841 atggaggccg gccgcagcct gtttggctcc atcctgtttt atttcagttt cttcaccatc
    12901 ctcaccaata tcggggcggt gctggtttat gtatcgctgc tgtcgcccag cggctatgcg
    12961 tggcttccgg ccttcgccag cccacgcatg cgtgcgggcg tggctgtcgc aatcgccgtg
    13021 gttttcctcg tttacatcac cgtgctggcg cccttgcaga agctggaagg cgcctctgcg
    13081 ctgtgcaaca tccttctgca ctatggtgcg ccggtgatat tcgttttgtg gtggctcctg
    13141 gccggcagag atggctccag ccggtggagc gacatcgcct tgtggctggt ctaccccctc
    13201 gcctatctgg cttatgcgct ggttcaagtg ccgttcaccg gggtcgtgct ctacccattc
    13261 ctcgacatgg cggcgaacgg cgttgccggc gttgccttgt ccgcgctgac gatcacggcg
    13321 gtttttcttg tgatatccgt actggccatc cttgcggatc gccatatttc acgctctagc
    13381 aaagcctgac gtcgaaccaa ccgtggtcgc agactggaga atccgagcat ttccttgaag
    13441 cctgatgttt atccaaaagc cctgccatgg ctggccggcg cggtaattct tttcggcatt
    13501 ggcctgcttc tcggctggta ctatcaattc tatcctgagc tgagagcaga gactttcgac
    13561 gaaaacggtc cagtcgaaac gcttgagatg gtggcgctga ttgcggcggc catcatattc
    13621 gcgtttcgcg cagtccgctc caacgaccca atcggcacga tgtgcatccc catcgtgctg
    13681 attctggcga tcgcgttcgt gcgcgaaacg ccgcgctgcg acagttcgtt ctacgatggc
    13741 ggcctgtgct ttcgcacgga acgctggaag gacgtcaccg tcacattgtt ccttattgcg
    13801 gccgccattg ccttgttccg ccggcggcag catctggccg ccgtgctttc gatccgcaaa
    13861 atacatgtat tctggccgat ctggctatct gccgtactgc ttattctggc agagataggc
    13921 gaggcgcatg gcgtgctggg aacggaagaa tccctggaat tcttcgccta tctttatctg
    13981 gcgtcgctgg gtgtctggat acaccgcaac acctgacgaa acaaacccgc ccctgggccg
    14041 gtttccaccg tttctgaagc cggcaggggg tgtaaccggc cggcgcttac gctattagga
    14101 cgccgataga aatctggacg cgatctttcg atgaccattt ccaataccgc gccgatcacc
    14161 gctgaactga tcgcggcgca tgggctgaag cctgacgaat accagcgcat cctcgacctt
    14221 gtcgggcgtg agccgagctt tacagaactt ggtatttttt cggcgatgtg gaacgagcac
    14281 tgctcctaca aatcatccaa gaaatggctg cgcaccttgc cgaccaccgg cgcggtcgtc
    14341 atccaggggc ccggcgaaaa tgccggcgtg gtcgatattg gcgacggcga ttgcgtagtc
    14401 ttcaagatgg agagccacaa ccacccgtcc ta
//
LOCUS       DASYCS010000005         2431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_146119,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000005 DASYCS010000000
VERSION     DASYCS010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146119"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..1041)
                     /locus_tag="VGM46_00205"
     CDS             complement(37..1041)
                     /locus_tag="VGM46_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007199816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HEY4191042.1"
                     /translation="MTPSLTVTDMHTGGEPVRIVTGGYPAIPAGTILEKRAYVRDNLD
                     HLRKMLMFEPRGHYDMYGVLPVEPDLPGADMAVLFMHNEGYSTMCGHAVIALGRYAVD
                     QGIVAKTEPVTIVNIEAPCGLIVASVEVEGGKAGAVSFESVPAFLFARDREIILPGAG
                     KIRFDIAYGGAFYALADCRQFGLEFGKDRARDFVDAATALTDRLKAEFPLAHPDHADL
                     GFLYGTILTDGKDAFRAEPTKNVCVFAEAEVDRSPTGSGVTARLAAMHAKGEIGLGQT
                     RMFESIVGSRFSGAVARITKAGPHEAIVARVGGRAFYSGKAEYVVEPDDELGRGFLLR
                     "
     gene            complement(1038..1841)
                     /locus_tag="VGM46_00210"
     CDS             complement(1038..1841)
                     /locus_tag="VGM46_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEY4191043.1"
                     /translation="MDKGTVFRDLHASTFIIPNPWDIGTAKLLTSFGFKALATTSAGF
                     AFSRGLPDGGVAFEAMIAHCKELSAATDLPVSADLEKGKGDSAERAAETIFAAEAAGL
                     AGCSLEDFSGDETRPIYDFSHAVERVSAAVEAARSLKRDFVFTARAENYLHGRPDLDD
                     TIRRLQAFEKAGADVLYAPGITDIETVRILCSSVSKPVNVLAVPGFTMTELAQAGARR
                     ISLGSKLTNCAFGLLERASREMLEQGTFGFANDAMAFARLQKMFSTGDA"
     gene            complement(1979..>2431)
                     /locus_tag="VGM46_00215"
     CDS             complement(1979..>2431)
                     /locus_tag="VGM46_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEY4191044.1"
                     /translation="FVERWYRELPAIFGSGVEKIPYVETFIAAVEAAGVAHCVASSAR
                     LEKMHMTLGQTGLLAHFRDVLFSATMVAHGKPAPDLFLHAARTMGAAPADCIVIEDSV
                     AGTKAGIAAGMRVFSYHGDPFSDGEGLADAGGILFDDMRKLAALIPLL"
BASE COUNT          459 a          751 c          816 g          405 t
ORIGIN      
        1 atttgcaaac ggccatgccg gcgatgcggc ggcgattcag cgcagcagga agccgcgccc
       61 gagttcgtcg tccggctcga cgacgtattc ggccttgccc gagtaaaagg cgcggccacc
      121 gacacgggcg acgatcgcct cgtgcggacc cgccttcgtt atgcgcgcaa ccgcgccgga
      181 aaagcgcgat ccgacgatgc tctcgaacat gcgggtctgg ccgaggccga tctcgccttt
      241 ggcatgcatc gccgccaggc gagcggtgac gccggaaccg gtcggcgagc ggtcgacctc
      301 ggcctcggca aagacgcaga cgtttttggt cggctcggcg cggaaggcgt cttttccgtc
      361 ggtcaggatg gtgccgtaga gaaagccgag atcggcatgg tcggggtggg caagcgggaa
      421 ttcagccttc agccggtcgg tcagcgccgt tgcggcgtcg acaaaatctc gggcgcggtc
      481 cttgccgaat tccagcccga actgccggca gtcggccaag gcatagaacg cgccgccata
      541 ggcgatgtcg aagcggatct tgcccgcacc cggcaatatg atttcccggt cgcgggcaaa
      601 caggaaggcc ggcacgcttt cgaacgacac cgcgcctgcc ttgccgccct ccacctcgac
      661 cgaagcgacg atcagcccgc acggcgcctc gatgttgacg atcgtcaccg gctcggtttt
      721 ggcgacgatg ccctgatcga ccgcatagcg cccaagcgcg atgacggcgt ggccgcacat
      781 ggtggaatag ccttcattgt gcatgaacag cacggccata tcggcgcccg gcaaatccgg
      841 ttcgaccggc agcacgccgt acatgtcgta atggccgcgc ggctcgaaca tcagcatctt
      901 gcgcagatgg tcgagattgt cgcggacata ggcacgcttt tcgaggatgg tgccagcggg
      961 aatcgccgga tagccgccag tgacgatgcg caccggctcg ccgccggtat gcatgtcggt
     1021 gaccgtcaat gaaggcgtca tgcgtcgccc gtggagaaca ttttctgcaa gcgggcgaat
     1081 gccatggcgt cgttggcgaa gccgaaggtg ccctgttcca gcatctcgcg ggaagcccgc
     1141 tccagcaggc cgaaggcaca gttggtcagc ttcgagccaa gcgagatgcg cctggcgccc
     1201 gcctgcgcca gttcggtcat ggtgaagccc ggcacggcaa gcacgttgac cggcttcgac
     1261 accgaggaac agaggatgcg tacggtttcg atatcagtga tgccgggcgc ataaagcaca
     1321 tcggctccgg ccttctcgaa ggcctgcaaa cgcctgatcg tatcgtcgag gtccggcctg
     1381 ccatgcaggt agttttcggc gcgcgcggtg aagacgaaat cacgcttcag cgagcgcgcc
     1441 gcctcgacgg cggccgagac gcgttcgacg gcatgggaaa aatcgtagat cggcctggtc
     1501 tcatcgccgg agaaatcttc aagcgaacag ccggccaatc cggcggcttc ggcggcaaag
     1561 atggtttcgg cggcgcgttc ggcgctgtcg cccttgcctt tttcgagatc ggcggaaacc
     1621 ggcaggtcgg ttgccgccga cagttccttg caatgcgcga tcatcgcctc gaaggcgacg
     1681 ccgccatccg gcaagccgcg cgaaaaggca aagccggcgc tggtggtggc gagcgccttg
     1741 aagccgaacg aggtcagaag cttcgcggtg ccgatatccc agggattggg aatgatgaag
     1801 gtggaagcgt ggagatcgcg gaaaacggtg cccttgtcca tgaagcctca tccttgcccg
     1861 agctgaaaca gaggccgagc gtatcgcgtt gcagggttgc gggcaaacgg atgtcttcgg
     1921 tccaggcgcg gcttttcgcc cggcccgccg ctgcggcgac gggaagcggt cggcgggcct
     1981 acaacagcgg gataagtgcg gccagcttgc gcatgtcgtc gaagaggatg ccgccggcat
     2041 cggccaatcc ttcgccgtcg gaaaagggat cgccatggta ggaaaatacc cgcatgcccg
     2101 ccgcgatgcc ggctttggtg ccggccacgc tgtcctcgat gacgatgcag tcggccggcg
     2161 cggcgcccat cgtcctggcg gcatgcaaaa acaggtccgg ggccggcttg ccatgcgcga
     2221 ccatggtggc gctgaacagc acgtcgcgga aatgcgccaa aagaccggtc tggccaagcg
     2281 tcatgtgcat cttttcgagg cgcgccgagg aagcgacgca atgcgcgaca ccggccgctt
     2341 cgacggcggc gatgaacgtt tcgacatagg ggattttttc gacgccgctg ccgaagatgg
     2401 cgggcagttc gcgataccag cgctcgacga a
//
LOCUS       DASYCS010000006        23963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4059663,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000006 DASYCS010000000
VERSION     DASYCS010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 23963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23963
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4059663"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="VGM46_00220"
     CDS             complement(<1..763)
                     /locus_tag="VGM46_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019863105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY4191045.1"
                     /translation="MTEQVVKTFTDPQFLIALLVGLAVFATVFTLLPALGGNPLKARM
                     KSVALERDELRAKQRARLAAEADRRRKGLREEQSIGMRNIVDRLDLRRALADEGTMQK
                     LKVAGFRGQNPLTRFLFFRLVLPFVGFALAAAYIFVLGGMADKPLFAKVFVCILAAYA
                     GFYAPNLYVSNRASKRKKSIQAAWPDALDLMLICVESGMSVEAAMRKVADEIGVQSGE
                     LAEEFVLTNAELSYLQERKQAYDNLASRTGLESVKS"
     gene            complement(781..1794)
                     /locus_tag="VGM46_00225"
     CDS             complement(781..1794)
                     /locus_tag="VGM46_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019863104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY4191046.1"
                     /translation="MFGIDTTVLAFVLLAGFSAGAVAYAFLFKRIDNEKTTGRRLETV
                     RTLETDRSVVKASRDRVAEAAKRRKSVQDSLKELDQKQKTRDVNVKKPPLKVQIRQAG
                     MTVSMERFYLYSAICGIVVAAVAYLAGAPLIIVPGALIAGALGLPRWFVSFLRGRRIK
                     RFLNEFPNALDIIVRAIKSGLPLNDGIRLIANEAPEPVKSEFRRIVDSQQLGLSIPEA
                     ALRMTETMPCPEAGFFGIVIQIQSQAGGNLSEALGNLSRVLRDRKKMKAKVQALSMEA
                     KASAVIIGALPFIVATMIYLSSPNYIMPLFTTNVGNIILGISGLWMSMGILVMRKMMN
                     FEV"
     gene            complement(1812..3314)
                     /locus_tag="VGM46_00230"
     CDS             complement(1812..3314)
                     /locus_tag="VGM46_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HEY4191047.1"
                     /translation="MFGKRGNEDGGRPAPAARPPAADPSATAARATETAVLARPAASA
                     PPASSAPPARRSVEAPPLAPEPKRAQRERSESYYDTKSQVFSALIDTIDLSQLAKLDP
                     DSAREEIRDIVNDIIAIKNFAMSIADQEELLDDICNDVLGYGPLEPLLARDDIADIMV
                     NGSKNVYIEVNGKVEQTSIRFRDNQQLLNICQRIVSQVGRRVDESSPICDARLPDGSR
                     VNVIAPPLAIDGAALTIRKFKKDKLTLDQLVKFGTITQHGADVLKIIGRVRCNVIISG
                     GTGSGKTTLLNCLTNYVDRDERIITCEDSAELQLQQPHVVRLETRPANLEGEGEVTMR
                     ELVKNCLRMRPERIIVGEVRGPEVFDLLQAMNTGHDGSMGTIHSNSPRECLNRIESMI
                     AMGGFTLPQKTVREIIVGSVDVIIQASRLRDGSRRITHITEVIGMEGDVIITQDLVLY
                     NIKGEDANGRLLGEHVSTGIGRPHFWDRARYFNEEQRLAAALEAMEKRAD"
     gene            complement(3342..4628)
                     /locus_tag="VGM46_00235"
     CDS             complement(3342..4628)
                     /locus_tag="VGM46_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaE family protein"
                     /protein_id="HEY4191048.1"
                     /translation="MTMSNLAYDTSLDGDGAHQEMSMMQALRPIPRISIQAFCETEGV
                     AGPIERAGEDRRMARAHLKVHMGGIATAIEFYQSAPTPNLILLESRSEPKQLLEQLAQ
                     LSEYCDPTSKVVVIGHYNDVGLYRELIRSGISEYVIAPISMADIVSVISSIFVDPEAA
                     PIGRSVAFIGAKGGVGSSTIAHNVAWTMSALFKSEVVVADLDLAFGTANINFDQDPAQ
                     GIAEAVFSPERIDEVYLDRLLAQCAEHLSLLAAPSTLDRVYDFDADAFSHIIETAQRT
                     APLLVLDVPHVWTGWTKNTLTKADEVVITATPELANLRNAKNLVDMLKRLRPNDPPPK
                     LIINQTGMPKRPEISADDFAEPLGLVPMSVIPFDPQLFGNASNNGRMLGEMDAKSPIV
                     GMINEVAHVLTGRSEIRSKKKAALGGLLDRLKRNKK"
     gene            complement(4661..5398)
                     /locus_tag="VGM46_00240"
     CDS             complement(4661..5398)
                     /locus_tag="VGM46_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006337898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaD family pilus assembly protein"
                     /protein_id="HEY4191049.1"
                     /translation="MSHSAFEAKMKIAAKPSSRSRMYLLAAPVLAVAAVGLLSGCADR
                     DSITVGAIPDDYRTNHPIVISEKEEKIDLPVGARDRGMTQSQRATLLGFLDGYDKSAG
                     SILTIMVPSGSANEVAAAAAARDFARLAAKTGIRSGRIVKTSYQAGSSEVSAPVRVSF
                     SRLRAHTDACGRWPEDLLETSENKHYADFGCSYQNNLAAQMANPADLIGPRKQTSIDA
                     ANRSKVIDSYRDRGISQEFLRNSEIDY"
     gene            complement(5413..6894)
                     /locus_tag="VGM46_00245"
     CDS             complement(5413..6894)
                     /locus_tag="VGM46_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015319332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein family
                     protein"
                     /protein_id="HEY4191050.1"
                     /translation="MRTTTKLPVVVAMAASLTLVGAYKPGVSEANAASNAQVSASKAT
                     QRIKLGLNKSVVIDLPNDAYDILVANPAVADAVTRTSRRIYLFGKAVGETNIFVFGPN
                     GEQIVSLDLAVERDVAGLDNYIKRFIPTSDVKVELLNDNVVLTGTVDTPLDAKRAEEL
                     ATIFVSGGEATTGQYSQTAAGGSSQSGVDIDNPDSQRRTSKIVNLLQIIGEDQVTLKV
                     TVAEVSRNVMKQLGVKLIGQADGISFQSLASAAGSMVNAGTSGAGVGGASYLKGTMTA
                     MEQSGVLKTLAEPTLSAVSGEKATFKVGGEYNVVVGQTNDNGTVVPTLQQIEYGIGLE
                     FQPVVLSAGRISLKVRTSVSEPTTEGTQIAGTNVVSLRKRLADTTVELPSGGSMMIAG
                     LVNDDVRQTVDAVPGLSKIPVLGTLFRSRDFVRNESELVIIITPYLARPVARNDLAKP
                     DDNFTPASDGAGMFLGKVNRVYGTMQTDMPNGRYHGVVGFIYK"
     gene            complement(6891..7706)
                     /gene="cpaB"
                     /locus_tag="VGM46_00250"
     CDS             complement(6891..7706)
                     /gene="cpaB"
                     /locus_tag="VGM46_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010913352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEY4191051.1"
                     /translation="MPASRLIILGVAVAAASGAGYVAKNMVAKAPPAAVVDAGPQAPA
                     IALQDVLVLSGDVAMGDPVGSNISWQPWPAEGINANFITRAAEPDAVEKLKDSVARMA
                     MFTGEPLRRSKLIGDGQSFMSSILPAGSRAVATSIAADTSAGGFILPNDFVDVIMTRR
                     ADANSGGSGFTTETILKNIRVLAIDQTIQEDEEGKKTRVGQTATLELTPQQAEIITVA
                     QQMADRLTLSLRSIKDTQEKITEEADYLVSGNGRKGTVRLIKSGEVSEVGARK"
     gene            complement(7878..8393)
                     /locus_tag="VGM46_00255"
     CDS             complement(7878..8393)
                     /locus_tag="VGM46_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEY4191052.1"
                     /translation="MLEAAIFVVFPFCMLFAAISDALSMTIANRVPLLLVAVFAIVAP
                     LTGMEWTAYGMHFAAGALVLCVTFGLFALGGMGGGDAKLLAGTALWMGFNIHLLEYLV
                     VSTALGGLLTIAILAYRKSALAVFTRQSSFLAHVANEANGVPYGIALGIGGLITYPDS
                     PLMVWALQQLA"
     gene            8590..9012
                     /locus_tag="VGM46_00260"
     CDS             8590..9012
                     /locus_tag="VGM46_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019863097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HEY4191053.1"
                     /translation="MAKFRSSLLVAAAFFAAASAVPATAGQGIEVTMNQAKIVKLARP
                     ADTIVVGNPAIADASVQDASTVVLTGKGFGITNLVIMDGQGVPIIDEQVTVVRQNASS
                     VRIYRRAEVQTMSCTPYCESSYKNDAEKASETELNATP"
     gene            9169..9768
                     /locus_tag="VGM46_00265"
     CDS             9169..9768
                     /locus_tag="VGM46_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006337908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEY4191054.1"
                     /translation="MNTPSEPESELREARRPGFLGRFLRDRSGSTAIEFVILAIPFSM
                     LVFAILESCISFAGQEVLANAADDVARQVRTGQLRAATITKESIQQAICNKLAVMVGN
                     SCNDRIFIDLRKYDTFQEAAAARFKIINNAIVLTKDNVKDPLGYTISPGPAMSINMLR
                     VFYKWPVMTDFVSTWAAKLDGPNTLHFATVTWQNEPFDE"
     gene            9807..10430
                     /locus_tag="VGM46_00270"
     CDS             9807..10430
                     /locus_tag="VGM46_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEY4191055.1"
                     /translation="MLFGVGARRDIAKFFSGAVRFCTDRRGVAAIEFAFIAPVLFIMY
                     FVTMEVAQGIEANKKVSRIGSMVADLVTQRPTVTPSDLDAIMRIADSTLQPYNRSSPK
                     IVITEITISADAKPKATVAWSRKMENGAFSKGDPAKTPVTIPTSLNIGGTFLVRVDSY
                     LNYQPILTWTAEQKQATGLFASFDNLPMSETYYLRPRMSTSVDCATC"
     gene            10556..11797
                     /locus_tag="VGM46_00275"
     CDS             10556..11797
                     /locus_tag="VGM46_00275"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEY4191056.1"
                     /translation="MARAFVFVLDSFGIGGAADAARYGDEGADTFGHIAQACAAGEAN
                     RHGLRSGPLAVPNMAALGLWRASGIATGKGLEPHRAKLLASSFHGAAQEISSGKDTPS
                     GHWEIAGLPARFDWGYFPDTVPAFPASLTDAMIREGGVPGILANRHAPGTTVIEDFGE
                     EHIRTGKPICYTSVDSVLQIAAHESHFGLERLYEFCELVRRLCDPLKIGRVIARPFLG
                     ETAVTFQRTPNRRDFAIPPPEPTLLDRLTADGRKVIAIGKIGDIFAHRGISEIRKGAG
                     NMAMFDKALTAIDDAGDGDFVFANFVDFDTEFGHRRDVAGYAAALEAFDRRLPEALAR
                     LKPGDLLILTADHGNDPTWRGTDHTRERIPVIGIAPGLKGGDIGLRPTFADIGATVSQ
                     HLGLAPGAHGGSFLAAIGGHA"
     gene            11790..12680
                     /gene="mutM"
                     /locus_tag="VGM46_00280"
     CDS             11790..12680
                     /gene="mutM"
                     /locus_tag="VGM46_00280"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEY4191057.1"
                     /translation="MPELPEVETVRRGLQPVLEGARIIKVETRRPNLRFPFPERFCER
                     LTGQTIVSLGRRAKYLVMETKDGPGLICHLGMSGSFRIEQPENSETPGGFHYDRSRNE
                     AHDHVVFDVISATDIPARIVFNDPRRFGFMLFAEGAANEHPMLAGLGVEPTGNALDGA
                     LLASLFEGRKAPLKAALLDQSLIAGLGNIYVSEALWRAGLSPLRAAGTIAGTGKKQRE
                     QSARLADAIRSVIADAIAAGGSSLRDYVQTDGSLGYFQHSFAVYGREGQPCPKPGCGG
                     HIERIVQSGRSTFFCRSCQH"
     gene            12782..13555
                     /locus_tag="VGM46_00285"
     CDS             12782..13555
                     /locus_tag="VGM46_00285"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEY4191058.1"
                     /translation="MAYETILTEVRGKVGLITLNRPQALNALNSQLLADLVEAVAAFE
                     TDAAIGCMVVTGSEKAFAAGADIKEMQSKSYIEAYLADFFGGWETFARARKPVIAAVA
                     GYALGGGCELAMMCDFIIAGDNAKFGQPEITLGVIPGMGGSQRLTRSVGKAKAMDLVL
                     TGRMMDAEEAERCGLVSRIVPVASLVEEALKAAEKIAGFSLPAVMMAKEAVSRSYETT
                     LAEGLRFERRLFHSMFALADQKEGMAAFSEKRKPNFTNR"
     gene            13710..13976
                     /gene="rpsT"
                     /locus_tag="VGM46_00290"
     CDS             13710..13976
                     /gene="rpsT"
                     /locus_tag="VGM46_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010913342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEY4191059.1"
                     /translation="MANTSSAKKATRKIARRAVINKNRRSRVRTYVRQVEEALAAGDK
                     VAAAAAFKAAEPELMRAATKGVLHKNTASRKVSRLASRLKTLSA"
     gene            14775..16310
                     /gene="dnaA"
                     /locus_tag="VGM46_00295"
     CDS             14775..16310
                     /gene="dnaA"
                     /locus_tag="VGM46_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019863089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEY4191060.1"
                     /translation="MQSVDRDFNDDIPLPANLTGGNRMAASAEAEQRFERIKVQLKAR
                     LGSEVYSSWFGRMKLAEASKGIVRISVPTAFLRSWINGHYLDVIADLWKQEDPEVLKI
                     DIIVRSATRHVVRNSEADVAMAPKASRHTPAALAAGTTAGRLERPGIAHGRPVDMEMR
                     QSVLGSPLDPRYTFGSFIEGPSNRVAFAAARAVAESQSSAVRFNPLFLHASVGLGKTH
                     LLQAIAAESLRQNPKSRVVYLTAEYFMWRFATAIRDNNALTLKEQLRDIDLLIIDDMQ
                     FLQGKSIQHEFCHLINMLLDSAKQVVVAADRPPSELESLEPRVRSRLNGGVALEMSAP
                     DFTMRLGMLKLRLETAATEGTPLDISEEILNHVARTVTGSGRELEGAFNQLLFRQSFE
                     PQITIDRIDEILGHIYRTGEPKRVRIEDIQRIVARHYNVSKTELLSNRRTRTIVKPRQ
                     IAMYLAKVMTPRSLPEIGRRFGGRDHTTVLHAVRKIEDLSGDGNTLSQELELLRRLIN
                     DQA"
     gene            16511..17629
                     /gene="dnaN"
                     /locus_tag="VGM46_00300"
     CDS             16511..17629
                     /gene="dnaN"
                     /locus_tag="VGM46_00300"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEY4191061.1"
                     /translation="MRVILERSNLLKSLNHVHRVVERRNTIPILSNVLLAAEGASLEM
                     KATDLDLEITEATPAKVEQGGATTVPAHLLYDIVRKLADGAEVMLRTDEDGNAMTVVS
                     GRSSFRLQCLPQSDFPELSAGSFSHIFRLDSAGLKGLIEKTQFAISTEETRYYLNGIY
                     LHAHEVGGKLKLRSVATDGHRLARAEIEAPAGSEGMPGIIIPRKTVSELQKLVDDPDV
                     AVTTELSETKIRFTIGSVVLTSKLIDGTFPDYQRVIPTGNDKKLIIDRQSFSAAVDRV
                     STISSERGRAVKMSIVEGQVTLAVNNPDSGSATEELAADYSSDAIEIGFNARYLLDVA
                     AQLTGSEARFMLADAGSPTLIHDTSDETTLYVLMPMRV"
     gene            17890..19044
                     /gene="recF"
                     /locus_tag="VGM46_00305"
     CDS             17890..19044
                     /gene="recF"
                     /locus_tag="VGM46_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010913339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEY4191062.1"
                     /translation="MTEQTPNATNQTHIRKLTLTNFRNYAALSIDLRPGAVVFTGDNG
                     AGKTNLLEAISLLTPGRGLRRAPYGDIAREGGDGGFALHAAIDGPQGEIEIGTGIASG
                     DATRRVRINSAPARSADAMLEWLRVVWLTPAMDALFTGPAADRRRFLDRLVLAIDAGH
                     GQRALDYEKAMRGRNRLLADGSRNDAWFEAIETQMAETGVAIAAARAELVRLLAAMID
                     RLPGDSPFPQADLVLSGALEAQISSVAAVDVEERFRRELAGGRERDRAAGRTLDGPHR
                     SDLIVRHRPKAMPAELCSTGEQKALLVGIVLSHARLTGEMSGIKPILLLDEIAAHLDG
                     GRRAALFSILEELNCQAFMTGTEPSLFASLKGRAQFLSVDHGTVGPTQGS"
     gene            19076..19831
                     /locus_tag="VGM46_00310"
     CDS             19076..19831
                     /locus_tag="VGM46_00310"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010913338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HEY4191063.1"
                     /translation="MTLNDQELERYARHIVLPEIGGAGQQKLKRARVLVIGAGGLGSP
                     VLEYLAAAGVGTLGIIDDDIVSLSNLQRQVIHSTQAIGQAKIDSAREAILRINPHIAV
                     EAHTMRLTAQNAPALVRAYDIVVDGSDNFETRYAVADACAAEKRPLVHAALGRFDGSV
                     TVLMPFETAKDGRQNPSYRDLFPEPPPEGLVPSCAAAGVLGALPGVIGTLQAMEAIKL
                     IAGIGEPLVGRLLLYDALAARFDTITYSRRKEG"
     gene            complement(19828..20829)
                     /locus_tag="VGM46_00315"
     CDS             complement(19828..20829)
                     /locus_tag="VGM46_00315"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEY4191064.1"
                     /translation="MAGKKKPLVVLTRKLPDPVETRMRELFDARLNIEDRPMTQPELV
                     AAMREADVLVPTITDTIDTALIEQAGPNLKLIANFGNGVDKIDVAAAAKKGITVTNTP
                     NVLTEDTADMTMALILAVPRRLAEGANVLTSDHKWPGWSPTWMLGRRIWGKRLGIVGM
                     GRIGTAVAKRAKAFGLSIHYHNRHRILPSVEEGLEATYWESLDQMLARMDIISVNCPS
                     TPATFHLLSARRLALLQPTAYIVNTARGDIIDEDALVKLIHDGKIAGAGLDVYENEPA
                     LNAKLLKQANRGKMLLLPHMGSATMEGRIDMGEKVIINIRAFFDGHRPPDRVLPLRA"
     gene            20986..21546
                     /locus_tag="VGM46_00320"
     CDS             20986..21546
                     /locus_tag="VGM46_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010913335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEY4191065.1"
                     /translation="MSGFASLRLALGAVLLGTMLFPVGAGAQGAASASAPSVTLGPSG
                     LPLPRFVSLKSGRVNSRVGPGVNYSVDWMYTKSGLPMEIVQEYDTWRRVRDADGSEGW
                     INQSLLSGRRTAIVAPWQRGKGAHINLLDDSDKDAAVVAILEPGVVGTIKACNGEWCE
                     MTFAGHTGWINQGSLWGAYPGEKVKD"
     gene            complement(21613..22011)
                     /locus_tag="VGM46_00325"
     CDS             complement(21613..22011)
                     /locus_tag="VGM46_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEY4191066.1"
                     /translation="MDKRVRDAGLRPTRQRIALADLLFAKGDRHLSAEELHEEALAAG
                     VPVSLATVYNALHQFTQAGLLRILAVEGSKTYFDTNTSDHHHFYLEGENRIFDIDAGA
                     VTVANLPPPPEGMEIANIDIVVRLRQKRCE"
     gene            22301..22816
                     /gene="fabA"
                     /locus_tag="VGM46_00330"
     CDS             22301..22816
                     /gene="fabA"
                     /locus_tag="VGM46_00330"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010913332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     FabA"
                     /protein_id="HEY4191067.1"
                     /translation="MAGKSSYGYEELLACARGELFGPGNAQLPAPPMLMFDRITDISE
                     TGGEFDKGYIRAEFDINPDLWFFPCHFIGNPIMPGCLGLDAMWQLTGFYLGWLGEPGK
                     GMALSTGEVKFKGMVTPSVKKIEYGVDFKRVMRGRLVLGIADGWLKADGDPIYKATDL
                     KVGLSKQEAAA"
     gene            22848..>23963
                     /gene="fabB"
                     /locus_tag="VGM46_00335"
     CDS             22848..>23963
                     /gene="fabB"
                     /locus_tag="VGM46_00335"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014766691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HEY4191068.1"
                     /translation="MRRVVVTGLGIVSSIGNNAGEVEASLRDARSGITFSESFAEHGF
                     RCQVWGAPNLDTTDLVDRRAARFLSQGGMWNHVAMKQAVADSGLEEADISGNERTGII
                     MGSGGPSTRTIVEAAEITLKNNSPKRIGPFAVPKAMSSTASATLATWFKIHGVNYSIS
                     SACSTSAHCIGNAAEMIQWGKQDIMFAGGHEDLDWTMSDLFDAMGAMSSKYNDNASSA
                     SRAYDVSRDGFVIAGGAGVLVLEELEHAKARGAKIYCELVGYGATSDGYDMVAPSGEG
                     AIRCMRQALATVSTPIDYINTHGTSTPVGDAKEMGAIREVFGEKMPHISSTKSLTGHS
                     LGAAGVQESIYSILMMQGRFIGESAHIENLDPEFEGMP"
BASE COUNT         4546 a         7389 c         7463 g         4565 t
ORIGIN      
        1 cggatttcac cgattcgagg ccggtgcggc tggccagatt gtcgtatgcc tgcttacgct
       61 cctgcaaata ggaaagctcg gcgttggtga ggacgaattc ttcggccagt tcccccgact
      121 ggacgccgat ctcatcggcg accttgcgca tggcggcttc caccgacatg cccgactcca
      181 cgcagatcag catcaggtcg agcgcgtcgg gccacgcggc ctggatcgac ttcttgcgct
      241 tgctggcgcg gttcgacaca taaagattgg gcgcgtagaa gcccgcatat gccgccagga
      301 tacagacgaa cacctttgcg aaaagcggtt tgtcggccat gccaccgagc acgaaaatgt
      361 aggcggcggc cagcgcaaag ccgacgaacg gcaggacgag ccggaaaaac aggaagcgcg
      421 tcagcggatt ttgcccacga aagcctgcca ccttgagttt ctgcatcgtg ccttcgtccg
      481 caagcgcgcg cctcaaatcc agccggtcga cgatgttgcg catgccgatg gattgctcct
      541 cgcgcaggcc tttgcggcgg cggtcggctt cggcggcaag gcgggcgcgc tgcttggcgc
      601 ggagctcgtc gcgctcaagc gccaccgact tcatgcgcgc cttgagcggg ttgccgccaa
      661 gcgccggcag cagcgtgaaa accgtcgcga aaaccgcaag cccgaccagc agcgcgatca
      721 ggaactgagg gtcggtaaag gtcttgacga cttgttcggt catgatgcca ctacctcaag
      781 tcagacttcg aaattcatca tcttgcgcat caccaggatg cccatgctca tccacaggcc
      841 ggaaatgccg aggattatgt tgccgacatt cgtggtgaac aacggcatga tgtagttggg
      901 gctgctcaaa tagatcatgg ttgcaacgat aaacggcaag gcgccgatga tgacggcgga
      961 cgccttggct tccatggaca gggcctggac cttagccttc atcttcttgc ggtcgcgcag
     1021 gacgcgggaa agattgccga gggcctcgga aagattgccg ccggcctgcg actggatctg
     1081 gatgacgatg ccgaaaaagc ccgcttcggg acatggcatg gtttcagtca tgcgcaaggc
     1141 ggcttcgggg atagacaggc ccagttgctg ggaatcgacg atgcgccgga attcggactt
     1201 gacgggctcg ggtgcttcat tggcgatcag gcgaatgccg tcgttgagcg gcaagcccga
     1261 cttgatggcg cgcacaatga tatcgagcgc gttggggaat tcgttgagga accgcttgat
     1321 gcggcggcca cgaaggaacg aaacgaacca gcgcggcagg cctagagcgc cggcaatcag
     1381 cgcgccgggc acgatgatca ggggagcgcc ggccagatag gcgacggccg cgacgacgat
     1441 gccgcagatg gccgaataga gatagaaccg ctccatggaa accgtcatgc cggcctgacg
     1501 aatctggact ttcagtggcg gcttcttgac gttgacgtcg cgggttttct gcttctggtc
     1561 gagttccttg agggaatcct gaaccgattt gcgccgcttg gcggcttcgg ccacccggtc
     1621 acgcgaagcc ttgaccacgg atcggtcggt ctccagcgtc ctgaccgttt caaggcgcct
     1681 gccggtcgtc ttttcgttat cgatccgttt gaacaggaac gcataagcga ccgcgcccgc
     1741 gctgaagccg gcaagcaaaa caaatgccaa tacggtggta tcaattccga acatggactg
     1801 ccgctttcat gtcagtcagc acgcttctcc atcgcctcca gggcggcggc gaggcgctgt
     1861 tcttcgttga agtagcgcgc gcgatcccag aaatgcgggc gtccgatgcc tgtcgagaca
     1921 tgctcgccga gcagccggcc gttcgcgtcc tcacccttga tgttatacag caccagatcc
     1981 tgcgtgatga tgacatcgcc ttccatgccg atcacttcgg tgatgtgggt gatgcggcgc
     2041 gagccgtcgc gcaggcgcga ggcctggatg atgacgtcga ccgagccgac gatgatttcg
     2101 cgcacggttt tctgcggcag ggtgaagcca cccatcgcaa tcatcgattc gatacggttc
     2161 aggcactcgc gcgggctgtt ggagtggata gtccccatcg aaccgtcatg gccggtattc
     2221 atggcctgga gaagatcgaa cacctctggt ccgcgcactt cgccgacgat gatgcgttca
     2281 ggccgcatgc gtaggcagtt cttgacgagt tcacgcatgg tgacctcgcc ctcgccttcc
     2341 agattggccg ggcgcgtttc cagccgcacc acatggggct gctggagctg caattcggcc
     2401 gagtcctcgc aggtgatgat gcgttcgtcg cggtcgacat agttggtcag gcagttgagc
     2461 agcgtcgtct ttcccgaacc ggtaccgccc gaaatgataa cgttgcagcg cacccggccg
     2521 atgatcttga gcacatcggc gccgtgctgc gtgatggtgc cgaacttgac caactggtcg
     2581 agcgtcagct tgtccttctt gaactttcga atggtaagcg ctgcgccatc gatggcaagc
     2641 ggcggcgcga tgacgttgac gcgcgagccg tccggcaggc gcgcgtcgca gatcggcgag
     2701 gattcatcga cgcggcggcc aacctggctg acgatgcgct ggcagatgtt gaggagttgc
     2761 tggttgtcgc gaaaacggat gctggtctgc tcgaccttgc cgttcacttc gatgtaaacg
     2821 ttcttggagc cgttgaccat gatatcggcg atgtcgtcgc gggccagcag cggctccagc
     2881 ggaccatagc cgagaacatc gttgcagata tcgtcgagca gttcttcctg gtcggcaatc
     2941 gacattgcga aattcttgat cgcaatgatg tcgttgacga tatcgcggat ttcctcacgc
     3001 gcgctgtcgg gatcgagctt ggcgagctgc gacaggtcga tggtgtcgat cagcgcggaa
     3061 aacacctggc tcttggtgtc gtagtagctt tcgctgcgtt cgcgctgcgc ccttttcggt
     3121 tcgggcgcca gcggcggcgc ttctaccgag cggcgggcgg gcggcgcaga tgaagcaggc
     3181 ggcgccgatg ccgccgggcg cgcgagaact gccgtttccg ttgcgcgcgc ggctgtcgcg
     3241 gatgggtccg ccgcgggtgg acgcgccgcc ggggctggcc ggccgccatc ttcattgcct
     3301 cttttaccga acatgatcga tacccaatcc cgctggatcg cttacttctt gttacgcttg
     3361 agcctatcga gcagtccgcc gagagcggcc tttttcttcg acctgatctc gcttcgtccg
     3421 gtcagcacat gggcgacttc gttgatcatg ccgacaatcg ggctttttgc gtccatttcg
     3481 cccaacatgc gcccgttatt cgaggcattg ccgaacaatt ggggatcgaa cgggatgacg
     3541 gacatcggca ccaggccgag cggctcggca aaatcgtcgg ccgaaatctc cggccgcttg
     3601 ggcatgcctg tctggttgat gataagcttg ggcggcggat cgttggggcg cagccgcttc
     3661 agcatgtcga ccaggttctt ggcgttgcgc aggttggcca attctggcgt ggcggtgatg
     3721 acgacctcgt cggccttggt cagcgtgttc ttggtccaac ccgtccagac atggggcacg
     3781 tccagcacca gcagcggcgc ggtgcgctgc gcggtctcga tgatgtgcga gaaggcgtcg
     3841 gcgtcgaaat catagacccg gtcgagcgtg gagggcgcgg ccagcagcga aagatgctcc
     3901 gcgcactggg ccagaagccg gtcgagatag acctcgtcga tccgctcggg cgaaaagacc
     3961 gcttcggcga tgccttgcgc cgggtcctgg tcgaaattga tgttggcggt gccgaaagcg
     4021 agatcgaggt cggcgaccac cacttccgac ttgaacagag ccgacatcgt ccaggcgacg
     4081 ttgtgggcga tggtcgatga accgacgccg cccttggcgc cgatgaaggc gacggaacgg
     4141 ccgatcggcg cggcttccgg atcgacgaag atcgacgaaa tgacgctgac gatatcggcc
     4201 atcgaaatcg gcgcgatgac atattcggaa atgcccgagc ggatcagctc gcggtagagg
     4261 ccaacatcgt tgtaatggcc gatcaccact accttcgagg tcggatcgca atattcggaa
     4321 agctgggcaa gctgttcaag cagctgtttc ggctcgctgc gcgattccag caggatcagg
     4381 ttcggcgtcg gcgcggactg gtagaactcg attgcagtgg cgatgccgcc catatgcacc
     4441 ttgagatggg ccctggccat gcggcgatcc tcgccggcac gctcgatggg accggcgacg
     4501 ccttcggttt cgcagaatgc ctggatggaa atgcgcggta tcggccgcaa ggcctgcatc
     4561 atggacattt cctgatgcgc gccatcgccg tcgagactgg tatcgtaagc cagattgctc
     4621 atcgtcatgt tctttccgtg accagcgtgc ccgtggcggc ttagtagtct atttccgagt
     4681 tcctgaggaa ttcctgcgaa atgccccgat cgcggtaaga gtcgatgacc ttgctgcggt
     4741 tggccgcgtc gatcgaagtc tgcttgcgcg ggccgatcag atcggcgggg ttggccatct
     4801 gcgcggcgag attattctga tatgaacagc cgaaatcggc gtaatgcttg ttttccgagg
     4861 tttcgagcag gtcttccggc caacggccac aggcatcggt atgcgcccgc aacctggaaa
     4921 agctgacccg cacgggcgcc gacacctcgg acgagccggc ctgataggac gttttcacaa
     4981 tgcggcccga gcggatgcct gtcttcgcgg ccaatcgggc gaaatcacgc gccgccgccg
     5041 ccgcggcgac ttcgttggcc gagcctgacg gcaccatgat cgtcaggatg gagcctgcgc
     5101 tcttgtcgta accgtccagg aaaccgagca gcgtcgcgcg ttgcgattgc gtcatgccgc
     5161 ggtcgcgggc gcccaccgga aggtcgatct tttcttcctt ttcggaaatc acgataggat
     5221 gattggtgcg atagtcgtcg ggaatggcgc cgacggtgat cgagtcgcgg tcggcgcaac
     5281 ccgacagcag gccgacagcc gctacggcca ggaccggcgc agccagaaga tacatgcgtg
     5341 agcggcttga gggctttgca gcgatcttca tcttggcctc gaatgctgaa tgagacatga
     5401 ccggttcccc gcttatttgt agatgaagcc gacgacgccg tggtaacggc cgttgggcat
     5461 gtcggtctgc atggtcccgt agacgcggtt gaccttgcca aggaacatgc ccgcgccatc
     5521 gcttgccgga gtgaaattgt cgtccggctt ggccaggtca tttcgggcaa cgggccgggc
     5581 caggtagggc gtgatgatga tgaccagttc gctctcgttg cggacaaagt cacggctgcg
     5641 gaaaagcgtg cccagaaccg ggatcttcga caatccaggc accgcatcga cggtctggcg
     5701 aacgtcgtcg ttgaccaggc ccgcgatcat catggaaccg cccgagggca gctcgaccgt
     5761 cgtgtctgca aggcgcttgc gcagggagac gacgttcgtg ccggcgatct gagtgccttc
     5821 ggtcgtcggt tctgagacgg aggttcgtac cttcaggctg atgcggcccg cggaaaggac
     5881 taccggttga aattcaaggc caatgccata ttcgatctgt tgcagcgtcg gaacgactgt
     5941 gccgttgtca ttcgtctggc cgacgacgac gttgtattcg ccgccaacct tgaaggtggc
     6001 tttttcgccg gatacggcgc tcaatgtcgg ctcggccagt gtcttcagga cgcctgactg
     6061 ttccatggcg gtcatggtgc ccttcaggta gctcgcgccg ccaacaccgg ctccgcttgt
     6121 acccgcattc accatggagc ctgccgccga cgccagggat tggaatgaaa tgccgtctgc
     6181 ctgaccgata agcttgacgc cgagctgctt catgacgttg cggctgactt cggccaccgt
     6241 caccttcagc gtcacctgat cctcgccgat gatctggagc aggttgacga tcttcgaagt
     6301 gcggcgctgc gaatcggggt tgtcgatgtc gacgccgctc tgcgacgagc cgccggcggc
     6361 ggtctgcgaa tactggccgg tcgttgcttc accgccggag acgaagatgg ttgccagttc
     6421 ctcggcgcgc ttggcgtcga gcggcgtatc gacggttccc gtcagcacga cgttgtcgtt
     6481 cagcagttcg accttgacgt cggatgtcgg tatgaaacgc ttgatgtagt tgtcgaggcc
     6541 ggcaacgtcg cgctcgacag ccagatccag gctgacgatc tgctcgccgt tcgggccgaa
     6601 gacgaagatg ttggtttcac cgaccgcctt gccgaacagg tagatgcgcc gcgaagtgcg
     6661 cgtgacggca tccgccaccg ccggattggc gacgagaatg tcgtaggcgt cgttgggcag
     6721 gtcgatcacc accgatttgt tgaggccaag cttgatcctc tgcgtcgcct tcgaggcact
     6781 gacctgcgca ttgcttgcag cattcgcctc gctgacgccc ggcttgtagg cgccgacaag
     6841 cgtgaggctg gcggccatgg cgacgacgac cggcagcttt gtggttgtcc tcatttcctt
     6901 gcccccactt cggaaacttc tccggatttg atcaggcgca ccgtgccttt gcggccatta
     6961 cccgaaacca ggtaatccgc ttcctcggtg atcttttcct gcgtgtcctt gatagagcgc
     7021 agcgacaagg tgagacgatc ggccatctgc tgcgccacgg tgatgatctc ggcctgctgg
     7081 ggcgtcaatt cgagcgtcgc cgtctggccg accctcgttt tcttgccttc ctcatcctcc
     7141 tggatcgtct ggtcgatggc gaggacgcga atgttcttca ggatcgtctc ggtggtgaag
     7201 ccgctgccgc ccgaattcgc atccgcgcgg cgcgtcatga tgacatcgac aaagtcattg
     7261 ggaagaatga agccgccggc cgacgtatcc gccgcgatgg aggtggcgac ggcgcggctg
     7321 cccgccggca ggatggagga catgaagctc tggccatcgc cgatcagttt ggagcggcgc
     7381 agcggctcgc ccgtaaacat ggccatgcgg gcaacggaat ccttcagctt ttccacagcg
     7441 tccggttcgg cggcacgggt aatgaaattc gcgttgatgc cttcggccgg ccagggctgc
     7501 cagctgatat tgctgccgac tggatcgccc atcgcaacgt cgccggaaag aacaagcacg
     7561 tcctgcaacg cgatagccgg cgcctgcggg cctgcatcga cgacagccgc aggcggcgct
     7621 ttcgccacca tgttctttgc gacatagccg gcaccgcttg cagcggccac cgccacgcca
     7681 agaattatca gtcgggatgc tggcatatgt ccgaacctcg cccttggcaa accacctagc
     7741 caagccggac attgcagcgg caatcgtcaa attagggtta atgtaatgct tacgatcgtg
     7801 attaatttaa cctttacata aattagaagc attgcctttg acggccactc cgccgtcttg
     7861 ccgcaaagcc tggatcatca ggccagttgc tggagcgccc agaccatcag gggcgaatcc
     7921 gggtaggtaa tcagcccccc tatccccagc gcgataccgt aaggaacgcc gttggcctca
     7981 ttggcgacat gggcgagaaa cgagctttgg cgcgtgaaaa cggccagcgc ggattttcgg
     8041 taggcgagaa tggcgatcgt gagcaggccg ccgagcgccg tcgaaacgac gagatattcc
     8101 agaagatgga tgttgaagcc catccacagc gcggttccgg ccaatagctt ggcgtcgccg
     8161 ccgcccatgc cgcccaatgc gaacagcccg aaggtgacgc acaagaccaa tgcgccggcg
     8221 gcaaaatgca tgccgtaggc ggtccattcc atgccggtga gcggcgccac tatggcgaaa
     8281 accgcgacca gcagaagcgg cacgcggttg gctatggtca tcgacaaggc atcggagatg
     8341 gcggcaaaca acatgcagaa cgggaagacg acgaatatag cggcttcaag catcggtttc
     8401 acacatagtt tagaaggccg gttgggttca aaaggccgga tggtgcgaat tcaaggctag
     8461 ccgaattccg ttaaggatcg atgagccagg cgtggatgag ggcataaggg cctgcgccat
     8521 tcacttaacc gttggttcac cattctcgtt cattgtccgt tgaacagtct gccgtctccg
     8581 gggagcacaa tggccaagtt cagatcatcc ttgctggtgg cagcggcctt cttcgccgcg
     8641 gcgtccgccg tcccggccac ggctgggcaa ggcatcgaag tcaccatgaa ccaggccaag
     8701 atcgtgaagc tggcgcggcc ggcggacacc atcgtggtcg gcaatccggc aatcgcggat
     8761 gcctccgtgc aggatgcatc gaccgtggtg ctgaccggca agggctttgg gatcaccaat
     8821 ctggtcatca tggacggcca gggcgttccc atcatcgacg agcaggttac ggtggttcgc
     8881 cagaacgctt cttcggtgcg catctaccgc cgcgcggaag tgcaaacgat gtcgtgcacg
     8941 ccctattgcg agagttccta caagaacgac gccgaaaagg cttcggagac ggaactgaac
     9001 gccacgcctt gagccgaaca gggcggaatt tgtctcgcaa ttgcctgatg ttgcgtggtt
     9061 agcgcgcggt aaaaccacat tcgccaagtt gaactttcat ccaaaccggc cttcaacagg
     9121 tttcggttac agtccttccc aacgatacga gaccagcagg gacgggacat gaacacccct
     9181 tcagaacccg aaagcgaatt gcgggaggca cggcggccgg gctttctcgg ccgcttcctg
     9241 cgcgaccgca gcggcagcac cgccatcgaa ttcgtgatcc tggcgatccc gttctccatg
     9301 cttgtctttg caattctgga aagctgcatt tcgtttgccg gccaggaagt gttggccaac
     9361 gccgccgacg acgtggcgcg ccaggttcgc accggccagc tgagggccgc aacgataacc
     9421 aaggaaagca tccagcaagc catctgcaac aagcttgcgg tcatggtcgg caatagctgc
     9481 aatgaccgca tctttatcga tcttcggaaa tacgacactt ttcaggaagc ggcggcggca
     9541 aggttcaaga tcatcaataa cgccatcgtc ctgaccaagg acaacgtcaa ggatccgctg
     9601 ggctatacga tctcgcccgg accggcgatg tcgatcaaca tgctgcgcgt gttttataaa
     9661 tggccggtca tgaccgattt cgtgagcacc tgggccgcca agctggacgg tcccaatacg
     9721 ctgcatttcg ccacggtgac gtggcaaaac gagccatttg acgaatgaac gggcagtgcg
     9781 gcgcggcgca taaaagggtc cttaaaatgc tgtttggggt gggggcacgc cgggatatcg
     9841 ccaaattctt ttcaggggcc gtccggtttt gcacggaccg gcgcggcgtc gcggcaatcg
     9901 agttcgcctt tatcgcgccg gtgctgttca tcatgtattt cgtgaccatg gaggtcgcgc
     9961 aaggcatcga agccaacaag aaggtcagcc gcatcggcag catggtggcc gatctggtca
    10021 cgcagcgccc caccgtgaca ccatccgacc tggacgccat catgaggatc gccgattcca
    10081 cgcttcaacc ttataacagg tcgtcgccga agatcgtcat caccgaaatc accatcagcg
    10141 ccgatgccaa gcccaaggcg acggtcgcat ggtcgcgaaa aatggaaaac ggcgccttta
    10201 gcaagggcga tccggccaag acgccggtga ccataccgac ctcattgaac atcggcggca
    10261 cgttcctcgt gcgcgtggac agctacctca actaccagcc gatcctgaca tggaccgccg
    10321 agcagaagca ggcgacgggg ctgtttgcct cattcgacaa cctgcccatg agcgagacct
    10381 attatctgcg cccgcggatg agcacgagcg tcgattgcgc cacctgctag ccgcgcagcc
    10441 gcgctgtttg ccgattgccg gcgcgttgtt tcgcgattgc cccttactgt ttggcgattg
    10501 ccaatgaata agcggaaccc tatgtcttgg ccgtcaggct gacatacggg tttcgatggc
    10561 gcgcgcattt gttttcgttc tggattcatt cggcatcggc ggcgcggccg acgccgcgcg
    10621 ttacggcgac gaaggggccg ataccttcgg ccatatcgcg caggcttgcg ccgccggcga
    10681 ggctaaccgc cacggcttgc gcagcggtcc gctcgcggtg cccaacatgg ccgcgctcgg
    10741 gctttggcgc gcatccggga ttgctaccgg caaaggcctt gagccgcatc gcgcgaaact
    10801 gctcgcctct tccttccatg gcgcggcgca ggagatttca agcggcaagg ataccccttc
    10861 gggccattgg gaaatcgccg gcctgccggc gcgtttcgac tggggctatt ttcccgacac
    10921 cgtacctgcc tttccagctt ccctgaccga cgccatgatc cgcgagggcg gcgtgccagg
    10981 tatcctggca aatcgccacg cgccgggaac gacggtgatc gaggatttcg gcgaggagca
    11041 tatccgcact ggcaagccga tctgctacac ctcggtggat tccgtactcc agatcgcggc
    11101 ccacgagagc catttcggcc ttgagcggct ttacgagttc tgcgagctcg tgcgcaggct
    11161 ttgcgacccg ctcaagatcg ggcgcgtgat cgcacggccg tttctcggcg aaaccgccgt
    11221 caccttccag cgcacgccca accgccgcga cttcgccatt ccgccgccgg agccgacgct
    11281 gcttgaccgg ctgaccgccg atggccgcaa ggtgatcgcc atcggcaaga tcggcgacat
    11341 cttcgcccat cgcggcattt cggaaatacg caagggcgcg ggcaacatgg cgatgttcga
    11401 caaggccctt accgccatcg acgatgccgg cgacggcgat ttcgtctttg ccaatttcgt
    11461 cgatttcgac accgaattcg gccatcgccg cgatgtcgcc ggctatgcgg cggcgctcga
    11521 agccttcgac cggcgcctgc cggaagcgct ggcgcggctg aagcccggcg atcttctcat
    11581 tctcaccgcc gaccatggca atgatccgac atggcgcggc accgaccaca cccgcgagcg
    11641 catcccggtc atcggcatcg cgcccggcct gaagggcggc gatatcggcc tgcgcccgac
    11701 ctttgccgat attggcgcga cggtgtcgca gcatcttggc cttgcgcccg gcgcacatgg
    11761 cggttccttt cttgcagcga taggcggtca tgcctgaatt acccgaagtc gaaacggtcc
    11821 ggcgcggcct gcaacccgtg ctggaaggcg cgcgcatcat caaggtggag acgcgacggc
    11881 ccaatctgcg ctttccgttt cccgaacgct tttgcgagcg gctgaccggc cagacgatcg
    11941 tgtcgcttgg ccgtcgcgcc aaatatctgg tcatggaaac gaaggatggt ccgggcctga
    12001 tctgccatct cggcatgtcg ggatccttcc ggatcgagca gccggagaat tccgaaactc
    12061 ccggcggctt tcattacgac cgctccagga acgaggcgca cgaccacgtc gtgttcgacg
    12121 ttatttcggc cacggacatt cccgcgcgca ttgtctttaa cgacccgcgg cgcttcggct
    12181 tcatgctgtt tgccgaaggc gccgccaacg aacatccgat gcttgccgga ctgggcgtcg
    12241 agccgaccgg caacgcgctc gacggcgcgc tgctggcatc gctgttcgaa ggccgcaagg
    12301 cacctctcaa ggccgccctt ctcgaccaga gcctgattgc gggactgggc aacatctatg
    12361 tgtcggaagc gctgtggcgc gcagggctgt cgccgctgcg cgccgccggc acgattgcgg
    12421 gaaccggaaa aaagcagcgc gagcaaagcg cccgcctggc ggatgccatt cgttcggtca
    12481 ttgccgacgc gatcgcggcc ggcggctcgt cgctgcgcga ttatgtgcag acggacggaa
    12541 gcctcggtta tttccagcat tccttcgccg tctatggccg cgagggccaa ccctgcccca
    12601 agcccggctg cggcggccat atcgaacgca tcgtccagag cggccgctcc accttctttt
    12661 gccgcagctg ccagcattga agatcgcacg ggccgcccac taaactgcac cggagggccg
    12721 cgttgaaggc gggccgccgt tgcggtagag caggagcctg tttgaatgcg gagacacact
    12781 catggcctat gagacgatcc tgaccgaggt gcgcggcaag gtagggctga tcacgctgaa
    12841 ccgcccgcag gcgcttaatg cgctgaactc gcagctattg gcggacctcg tcgaggcggt
    12901 ggctgcattc gagacggatg ccgctatcgg ctgcatggtc gtcaccggct cggaaaaggc
    12961 cttcgcggcg ggtgccgaca tcaaggaaat gcaatccaaa tcctatatcg aggcctatct
    13021 agcggacttt ttcggcggct gggagacctt tgcgcgcgcg cgaaaaccgg tgatcgcggc
    13081 tgtggccggc tatgcgctgg gcggcggctg cgaactggcg atgatgtgcg acttcatcat
    13141 cgcgggcgac aacgcaaagt tcggccagcc ggaaattacg ctcggcgtca ttccgggcat
    13201 gggcgggtcg cagcgcctga cgcgctccgt cggcaaggca aaggccatgg acctggtgct
    13261 gacggggcgg atgatggacg cggaagaagc tgagcgctgc ggtctggtct cgcgaatcgt
    13321 gccggtggcg tcgctggtcg aggaagccct gaaggccgcc gaaaagatcg ccgggttctc
    13381 gctgccggcc gtgatgatgg ccaaggaagc ggtcagccgc tcctacgaga cgacgcttgc
    13441 cgagggcctg cgcttcgaaa gacgcctttt ccactccatg ttcgcgctgg cggaccagaa
    13501 ggagggcatg gcggccttca gcgaaaaacg gaagccgaat ttcaccaatc gatgacggcc
    13561 ggcatgcgcc aaaaagacag gtgcgcagcg ttgacgcgcc gaaaaagctg aactataagc
    13621 cgcaccaacc gaggcggccc gtggctgccc gtttgttttt tgcgcgctac ggctttcgtg
    13681 gcgcttacca cagatcggaa agaggcgtca tggccaatac ctcctcggcc aaaaaggcca
    13741 cgcgcaagat tgctcgccgc gcggtcatca acaagaaccg ccgctcgcgc gtccgcacct
    13801 atgtgcgcca ggtcgaagag gcgctggccg ccggcgacaa ggttgccgcc gccgccgcct
    13861 tcaaagctgc cgagccggaa ctgatgcggg ccgccaccaa gggcgtgctg cacaagaaca
    13921 ccgcttcccg caaggtgtct cgcctggcta gccggctgaa gaccctctcg gcctgattcc
    13981 ctgtttttcg ttgacttctt aagcccggcg catcgcgtcg ggttttttcg tttgccgaca
    14041 gatgcttaaa aagaacctgt ccggcagcag ccggcggcat tgccggaatg tttgccgaca
    14101 tatgtgatgc ggtgaatata agccggcctg caaatggtcc gaaccatttg gtctctgcca
    14161 caaatagttc ttttacttca gttgcttatg acaggtcgtc cacaagttct gcacagcacg
    14221 cttcgcagga aggggacaag tcggtgtcaa gcaatttatt tcagaaaaaa ttattcgggc
    14281 cgccgatttt tcggttcgcc ggaaaagagt ttgattcaca atggctttac cgaaaagggg
    14341 ccgacaacca ggccgctcaa ggcctccgcg ctaaccagat tcgttaaaat tgtgttagtc
    14401 tcggccttgc cctttccaca gggatttcgg tagtgtccat ccatcgaaag ggacgggcca
    14461 gcggctctaa cctagtccaa gtcggccagc caaaattggc agaattcaaa acgcggagca
    14521 ataccgcgaa cgggatcggc ttgcctttcg acgtgtagat tggcgtagca gcgcgtagca
    14581 gacgagtacc cacggtttcg tcggctggca ccgctatgcc tttccgggga ctacagaggg
    14641 ttgcacgctc tgacgaccgg gaaattcgcg accagcggcg gcggcggcga atttcgcggg
    14701 ggacggcaag ttgccggacc aggatgcggg ggacgcatcc gcgatggaaa cagtacgaag
    14761 cgaggatttt gagcatgcag agcgtcgaca gggatttcaa cgacgatatc ccacttccag
    14821 caaacttgac cggggggaat cggatggcag cttccgcaga agccgagcag aggttcgaac
    14881 gcatcaaggt gcagttgaag gcccgcctcg gcagcgaagt ctattcgagc tggttcgggc
    14941 gcatgaagct ggccgaagcc tccaagggca tcgttcgcat ctcggttccc accgcctttc
    15001 tgcggtcgtg gatcaacggc cactatctcg atgtcatcgc cgacctgtgg aagcaggaag
    15061 accctgaagt cctgaagatc gacatcatcg tgcgcagcgc cacgcgccac gtcgtgcgaa
    15121 attccgaagc cgatgtcgca atggcgccca aggcaagccg gcacacgcca gccgcgcttg
    15181 cggccggcac cacggcaggc aggctcgaac ggcccggcat cgcgcatggc cgcccggtgg
    15241 atatggaaat gcgccagagc gtgctcggct cgccgctcga tccgcgctat actttcggct
    15301 cgttcatcga agggccgtcg aaccgggtcg cctttgccgc cgcccgcgcg gtcgcggaat
    15361 cgcagtcgag cgcagtgcgc ttcaacccgc ttttcctcca tgcctccgtc ggcctcggca
    15421 agacgcacct gttgcaggcg atcgcggccg aatcgcttcg gcaaaacccg aaatcgcgcg
    15481 tcgtctacct gacggcggaa tatttcatgt ggcgcttcgc caccgccatc cgcgacaaca
    15541 acgcgctgac cctgaaggaa cagctgcgcg acatcgacct gctcatcatc gacgacatgc
    15601 agttcctgca aggcaaatcg atccagcacg aattctgcca tctcatcaac atgcttctgg
    15661 atagcgccaa gcaggtggtg gtggccgccg accggccgcc gtccgaactg gaatcgctgg
    15721 agccgcgcgt gcgttcgcgc ctcaatggcg gcgtcgcgct ggaaatgtcg gctcccgact
    15781 tcaccatgcg ccttggcatg ctgaagctcc ggctggagac ggccgcgacg gaaggcacgc
    15841 cgctggatat ttccgaggaa atcctcaacc acgtcgcccg caccgtcact ggcagcggcc
    15901 gcgaactgga aggcgccttc aaccagcttc ttttccggca atcgttcgag ccgcagatca
    15961 ccatcgaccg catcgatgaa atcctcggcc atatctaccg caccggcgag ccgaagcggg
    16021 ttcgcatcga agacatccag cgcatcgttg cgcgccacta caatgtttcc aagacggagc
    16081 ttctgtccaa ccggcgcacc cgcaccatcg tcaagccgag gcagatcgcc atgtacctgg
    16141 ccaaggtgat gacgccgcgc tcgctgccgg aaatcggcag gcgtttcggg ggccgcgatc
    16201 acaccacggt tctgcatgcg gttcgcaaga tcgaggacct ttccggcgac ggcaatacgc
    16261 tgtcgcagga gttggaattg ctgcgcaggc tgatcaacga tcaggcgtga ggccaagcgg
    16321 ccgcgcccga tggccgcttt gcaacgcgtt atccccagaa aggcggcaag ggtgctgcga
    16381 tacggcggcg cgggcttgcg tttggatggg ttttcctgtc agtttgacgc aaatctgaac
    16441 ctgttcagac cttccgcggg ccgccgatag acggaggccc gcttgttcag tcaagtgagc
    16501 ctctatcgtc atgcgcgtca tcctggaacg gtcaaatctc ctgaagtcac tcaatcacgt
    16561 ccaccgcgtg gtcgagcgcc gcaataccat tccgatcctg tcgaatgtgc tgcttgccgc
    16621 cgaaggcgcc agccttgaaa tgaaggccac cgacctcgat ctcgaaatca ccgaagcgac
    16681 gcctgccaag gtcgagcaag gcggggcgac gaccgtgccg gcgcacctgc tctacgatat
    16741 cgtgcgcaag ctggccgatg gcgcggaagt gatgctgagg accgacgagg acggcaacgc
    16801 catgacggtc gtttccgggc gctcgtcctt ccgcctgcaa tgcctgccgc agtccgattt
    16861 cccggaattg tcggcggggt ctttctcgca catcttccgg ctggattcgg ccggtctcaa
    16921 gggtttgatc gaaaagacgc agtttgcgat ctcgaccgaa gagacgcgct attatctcaa
    16981 cggcatctac ctgcatgcgc atgaagtggg cggcaagctc aagctgcgct cggttgcgac
    17041 cgacgggcat cggcttgccc gcgccgaaat cgaggcgccc gccggctcgg aaggcatgcc
    17101 cggcatcatc attccgcgca agacggtcag cgagttgcaa aagctggtgg acgatcccga
    17161 tgttgcggtg acgaccgagc tttcggaaac caagatccgc ttcaccatcg gctccgtggt
    17221 tttgacctcg aagctgattg acggcacgtt tccggactac cagcgcgtca tcccgactgg
    17281 caacgacaag aagctcatta tcgaccggca aagcttttcg gccgccgtcg accgcgtctc
    17341 caccatttcc tccgagcgcg ggcgcgcggt caagatgtcg atcgtcgagg gccaggtgac
    17401 gcttgccgtc aacaatcccg attcgggcag cgcaacggaa gaactggcgg cggattattc
    17461 ctccgacgcg atcgaaatcg gcttcaacgc gcgctacctg ctggacgtcg ccgcccagct
    17521 taccggcagc gaagcgcggt tcatgctggc cgacgccggc tcgccgacac tcatccatga
    17581 caccagcgac gaaaccacgc tctacgtgct gatgccgatg cgggtctagg ccccgcacac
    17641 cgtcatgacg gtcgactatt cgccatggcc tgccggtctc gctcagactg atcctttggg
    17701 aacagcaatc ttatgcgcgt tcgccacggc cggtgacccc tccccacaag ggaaggtggg
    17761 actttgcggc gaccgcgcca ggtggattta ccggcaatcc gctcgcaaac gaatggcaaa
    17821 cgggttcccc tccccgttgt ggagtgcgct taagggtgaa ggtatcgagc gcgctgcctt
    17881 accggcatca tgaccgaaca aacccccaac gccacgaacc agacccacat ccgtaagctg
    17941 acgctgacca atttccgcaa ctatgcggcc ttgtccatcg acctgcgccc tggtgctgtg
    18001 gtgttcaccg gcgacaacgg ggctggcaag accaatctcc tggaggcgat atcgcttttg
    18061 acgccgggac gcggcctgcg ccgcgcgccc tatggcgata ttgcgcgcga gggcggcgat
    18121 ggcggctttg cgctgcatgc cgccattgat ggcccgcaag gcgagatcga aattggcacc
    18181 ggcatcgcca gcggcgatgc gacgcggcgc gtgcgcatca acagcgcgcc ggcgcgttcc
    18241 gccgacgcca tgctcgaatg gctgcgcgtg gtgtggctga cgccggcgat ggatgcgctg
    18301 tttaccggtc ccgccgcgga tcggcggcgc tttctcgacc ggctggtgct tgccatcgat
    18361 gccggccatg ggcagcgcgc gctcgattat gaaaaagcga tgcgcgggcg caaccgcctg
    18421 ctcgccgacg ggtcgcgcaa cgacgcatgg ttcgaggcga tcgaaaccca gatggccgag
    18481 accggcgtgg caatcgccgc cgcgcgcgcc gaactggtgc gcctgcttgc cgccatgatc
    18541 gaccgcctgc cgggcgacag ccccttcccg caagccgacc tcgtgctctc cggcgcgttg
    18601 gaggcgcaaa tttcctccgt cgccgccgtc gatgtcgagg agcgctttcg acgcgagctt
    18661 gccggcgggc gtgaacgcga ccgcgccgcc gggcgaacgc tggacggccc gcaccgttcc
    18721 gatctcatcg tgcgccatcg ccccaaagcc atgccggcgg aactgtgctc gacaggcgag
    18781 cagaaggcgc tgctggtcgg catcgtcctt tcccatgcgc ggctgaccgg cgaaatgtcg
    18841 ggcatcaagc caatcctttt gctggacgag attgcggcgc atcttgacgg cgggcggcgc
    18901 gccgccttgt tttcgatatt ggaagagctg aactgccagg ccttcatgac cggcaccgag
    18961 ccgtcgctgt ttgccagcct caagggccgc gcgcagttcc tgagcgtcga tcacggcacg
    19021 gttgggccaa cgcaaggctc ctgacaagca ccgcaagcgg ctttatgctc tatcgatgac
    19081 ccttaacgac caggaactgg aacgctacgc tcgccacatc gtgctgccgg agatcggcgg
    19141 cgccggccag cagaagctga agcgcgcgcg ggtgctggtg atcggcgcgg gcgggctggg
    19201 ttcgccggtg ctggaatatc tcgccgccgc cggcgtcggt acgctcggca ttatcgacga
    19261 cgacatcgtt tcgctttcca acctgcaacg gcaggtcatc cacagcaccc aggcgatcgg
    19321 ccaggccaag atcgacagcg ccagggaagc gatcctgcgg atcaatcctc atattgccgt
    19381 cgaagcgcac actatgcgcc tgacggccca aaacgcccct gcccttgtcc gcgcctatga
    19441 catcgtggtg gacgggtccg acaatttcga gacgcgctat gcggtcgccg acgcctgcgc
    19501 cgccgagaaa cggccgctgg tccatgccgc gcttggccgg ttcgacggat cggtcacggt
    19561 gctgatgccg ttcgaaacgg ccaaggacgg ccgccagaac ccgtcctatc gcgacctgtt
    19621 tccagagccg ccgccggaag ggctggtgcc ctcctgcgct gcggccggcg tgctcggcgc
    19681 cctgcccggc gtcatcggca cgttgcaggc gatggaggcg atcaagctga ttgccggcat
    19741 cggcgagcct ctggtcggac ggcttttgct ctacgacgcg ctggcggcgc gcttcgacac
    19801 gatcacctat tcgcgccgca aggaaggcta ggcccgcagg ggcagcacgc ggtccggcgg
    19861 gcgatggccg tcgaaaaagg cgcggatgtt gatgatgacc ttctcgccca tgtcgatgcg
    19921 cccttccatg gtggccgaac ccatatgcgg caaaagcagc atctttcccc gattggcctg
    19981 cttcagcagc ttggcgttca gcgccggctc gttctcgtag acgtcgagac ccgcgcccgc
    20041 gatcttgccg tcatggatca gcttgaccag cgcgtcctcg tcgatgatgt cgccgcgcgc
    20101 cgtattgacg atgtaggccg tgggttgcag cagcgccagc cgtcgcgccg aaagcagatg
    20161 aaaggtagcc ggagtcgaag ggcaattgac cgaaataatg tccatgcggg ccagcatctg
    20221 gtcgaggctt tcccaatagg tagcctcaag cccttcctcg accgaaggca aaatgcggtg
    20281 gcggttgtgg taatggatcg aaagtccgaa cgccttggcg cgcttggcga cggccgtgcc
    20341 gatgcggccc atgccgacaa tgccgagccg cttgccccag atgcgccgcc ccagcatcca
    20401 ggttggcgac cagccgggcc atttatggtc gctggtcaga acgtttgccc cctccgccag
    20461 ccggcgcggc accgccaata tcagcgccat ggtcatgtcg gcggtgtctt cggtcagcac
    20521 gttgggcgtg ttggtgacgg taatgccctt tttggcggcg gcggcgacgt cgatcttgtc
    20581 gacaccgttg ccgaaattgg cgatcagctt gaggttcggc ccggcctgct cgatcagcgc
    20641 ggtatcgatg gtgtcggtga tggtcggcac cagcacgtca gcctcgcgca tggcggccac
    20701 cagttcgggc tgcgtcatcg ggcgatcctc gatgttcagc ctggcgtcga acagttcgcg
    20761 catccgcgtc tcgaccggat cgggcaattt gcgcgtgagg acgacgagag gcttcttttt
    20821 gccggccatt ttaccctcga tccgggattg ttgagacctc tttaaccaag accggccaaa
    20881 ctgaaaaccg gtcgtggcac gaattcatcc tgtaacaagc ggtgccgccg atgacaaaga
    20941 aaaaggcgcc cggctgaggc caggctgttg gagcaaggaa caaacgtgtc tggtttcgcg
    21001 tcgcttcgcc tggccctcgg cgcagtattg ctgggcacga tgctgtttcc ggtcggcgcc
    21061 ggtgcgcaag gggcggcttc cgcctccgcg ccaagcgtga cgctcggccc gagcggcctg
    21121 ccgctgccgc gcttcgtcag cctcaaatcc ggccgcgtca attcacgggt cggacccggc
    21181 gtgaactatt ccgtcgactg gatgtatacg aaatcgggcc tgccgatgga gatcgtccag
    21241 gaatacgata cgtggcggcg cgtgcgcgac gccgacggct ccgaaggctg gatcaaccag
    21301 tcgctgctgt cgggccggcg caccgccatc gtcgcgccgt ggcagcgcgg caaaggcgcg
    21361 cacatcaatt tgctcgacga ttccgacaag gacgcagccg tcgtcgccat cctggaaccg
    21421 ggcgttgttg gcacgatcaa ggcatgcaac ggcgaatggt gcgaaatgac cttcgccggc
    21481 cataccggct ggatcaacca gggatcgcta tggggcgcct atccgggcga aaaggtcaaa
    21541 gactaagcgc taagctttgg tttcagggaa gctggcgctg gaaaattccg cgtggccttg
    21601 cgaattggcg ccttattcgc aacgcttctg gcgaagtctc accacgatat cgatattggc
    21661 gatttccatg ccttccggcg gcggcggcag gttggcgacc gtgacggcgc cggcgtcgat
    21721 gtcgaaaatc ctgttttcgc cctcaaggta gaaatgatga tgatccgagg tgttggtgtc
    21781 gaaataggtt ttcgaacctt ccaccgccag gatgcgcaac aggccagcct gggtgaattg
    21841 gtgaagcgcg ttatagacgg tcgcgagcga caccggcacg ccggcggcca gcgcttcctc
    21901 gtgcagttcc tcggccgaaa gatggcgatc gcccttggca aacagcagat cggccagcgc
    21961 tatgcgctga cgtgtcggcc ttagaccggc atcccgaacc cgcttgtcca cagcaactcc
    22021 ttacctcagc cagttcacgc cttcaggcag ttcacgttca ttcatcaccg caaggtggcc
    22081 gaatttcgta caacaagcct gaaacgcctg aaacacaaga ccgaatgccg gtgcctgcac
    22141 ttcaacatat attcgttcgg cgctatacga tcaatagcgc gcattgaccc ggccggagcg
    22201 gcaggctaag cgcaaatgtc ggttttcagc ctgaaacggc atgtcgggaa aacccaacgc
    22261 acgggcgcac gccgcccgac cacagtggag acagcgttcg atggcgggta aatccagtta
    22321 cggctacgag gaattgcttg cctgcgcacg cggcgagctt ttcgggccgg gcaacgccca
    22381 gttgccggct cctccgatgc tgatgttcga ccgcatcacc gatattagcg aaaccggcgg
    22441 cgaattcgac aagggatata tccgcgccga attcgacatc aatccggatt tgtggttctt
    22501 cccgtgccac ttcatcggca atccgatcat gcccggctgt ctaggcctcg acgccatgtg
    22561 gcagttgacg ggcttctacc tgggctggct gggcgaaccc ggcaagggca tggcgctgtc
    22621 caccggcgag gtgaaattca agggaatggt cactccttcc gtgaagaaga tcgaatacgg
    22681 ggtcgatttc aagcgcgtca tgcgcggccg gctcgtgctt ggcatcgcgg atggctggct
    22741 caaggccgat ggcgatccta tctacaaggc gaccgacctc aaggtcggcc tgtcgaagca
    22801 ggaagcagcc gcctgaccgg cgctcggact ctggaaggaa accacctatg agacgcgtcg
    22861 ttgtcaccgg cctcggtatc gtatcgtcta tcggaaacaa tgccggggag gttgaggcct
    22921 cgctgcgtga tgcgcgatcg ggcatcacct tctcggaatc ttttgccgag cacggtttcc
    22981 gctgccaggt atggggcgcg ccgaatctgg acacgaccga tctggtcgac cgccgcgcgg
    23041 cgcggttcct gtcgcagggc ggcatgtgga accatgtcgc catgaagcag gccgtcgccg
    23101 atagcggact ggaggaggcc gacatatccg gcaatgagcg caccggcatc atcatgggtt
    23161 ccggcgggcc ttcgacgcgc accatcgtgg aagccgcgga aatcacgctg aagaacaaca
    23221 gccccaagcg catcgggccg ttcgccgtgc ccaaggcaat gtcgtccacc gcttcggcga
    23281 cgctcgccac ctggttcaag atccacggcg tcaactattc gatttcctcg gcctgctcga
    23341 cctcggcgca ctgcatcggc aatgccgctg aaatgatcca gtggggcaag caggacatca
    23401 tgtttgcggg cgggcacgag gatctcgact ggaccatgtc ggacctgttc gacgcgatgg
    23461 gggcgatgtc gtccaaatat aacgacaacg catcgtcggc ctcgcgcgcc tatgatgtca
    23521 gccgcgacgg cttcgtcatc gccggtggcg ccggcgtgct ggtgctggag gaactggaac
    23581 acgccaaggc gcgcggcgcc aagatttatt gcgaactggt cggctatggc gccacttccg
    23641 atggctacga catggtggcg ccgtccggcg aaggcgccat tcgctgcatg cgccaggcgc
    23701 tggccaccgt ctccaccccc atcgactaca tcaacacgca cggcacctcc acgccggtcg
    23761 gcgatgccaa ggaaatgggc gcgatccggg aagtgttcgg cgaaaagatg ccacacatca
    23821 gctcgaccaa gtcgctgacc ggccattccc tgggcgccgc cggcgttcag gaatcgatct
    23881 attccatcct gatgatgcag ggccgcttca tcggcgaaag cgcgcatatc gagaatctcg
    23941 accccgaatt cgaaggcatg ccc
//
LOCUS       DASYCS010000007         4573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4929738,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000007 DASYCS010000000
VERSION     DASYCS010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4573
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4929738"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VGM46_00340"
     CDS             complement(<1..377)
                     /locus_tag="VGM46_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEY4191069.1"
                     /translation="MTATRTVAGRAPWPPLIYLAAIGVAALLGWSYPLPWIGGLLGDL
                     LFAIGWLVLLAVAVLWFTAFRAVTATRTTLCSNGLPEHLVTNGPFAVSRNPIYLANTL
                     LLIGVCFVGALPWFLPLAFVAALA"
     gene            complement(374..1363)
                     /locus_tag="VGM46_00345"
     CDS             complement(374..1363)
                     /locus_tag="VGM46_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1624 domain-containing protein"
                     /protein_id="HEY4191070.1"
                     /translation="MSIDVPSPAPSRRIAAIDVARGAALLAMASYHFTWDLEFFGYTE
                     PGLTGVGGWKIYARCIASTFLFLVGVSLVLAHGAGIRWKGFWKRLAMVAAAAAAITAA
                     TLYAFPDGFIFFGILHEITLASLLGLLFLRLPPLLTVVIAATVVALPFYLRSPIFDHP
                     SLWWVGLSTVNPHSNDYVPLFPWFGAVLAGIAATKLARSFGVLAWLGRFSPGRWAKPL
                     NFIGKHSLAFYLIHQPVLIACVWAFSQIMPAHVETRQENFLKSCQASCEVTRDTPFCT
                     TYCGCMFDMLARDDTLDRLYGADQSQELKDHVSNLAGLCTAKTDKAAVGGETP"
     gene            1647..2846
                     /locus_tag="VGM46_00350"
     CDS             1647..2846
                     /locus_tag="VGM46_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019858762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4191071.1"
                     /translation="MGTHVRHPMWLPSVRQAGARTFASLYTVESFARATVSSVIPIQA
                     YEILRSEQRVSILYTVVSLLGLSATLFMPMLIHRFARRWVYTAGACLLAIGSLFFLTD
                     TLPGQAIGMLCRVMGASALSITLNLYIMDHIRKTDFMRSESLRMAWSMVAWTGGPTLG
                     IFLYAHYGVYAAHGAVAAFAMLLLALFWIYRLGDNPLIAAGKSRAINPIANIGRFVAQ
                     PRLRLAWIIAFGRSCFWTTFFVYGPLFMVITGQGELAGGLLVSAGNGLLFMAIYWGRA
                     GRRYGARRTITLAFFAMAATLLAAGAVGEAVPLLTAALLLGGALFTIALDALGSPVFM
                     RAVHPYERAQMAAVYRTYLDFSELTPPMIYSVVLAFFGLGSVFATLGVLALFCGAISW
                     RYLPKAM"
     gene            2930..3442
                     /gene="cobU"
                     /locus_tag="VGM46_00355"
     CDS             2930..3442
                     /gene="cobU"
                     /locus_tag="VGM46_00355"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015317375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HEY4191072.1"
                     /translation="MPDRSLTFLIGGARSGKSAHAEQLAMCHAAPWSYIATAQAYDDE
                     MRERIATHRSRRGEGWTTIDAPLDLAGALDAVPEGCPVLVDCLTLWLTNHMLAGHDLS
                     AECARLEQSLMRPRGPWFVVSNEVGLGIVPENALARRFRDAAGRLNQQVAAVADTVLL
                     MVAGLPLKVK"
     gene            3442..4056
                     /gene="cobO"
                     /locus_tag="VGM46_00360"
     CDS             3442..4056
                     /gene="cobO"
                     /locus_tag="VGM46_00360"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEY4191073.1"
                     /translation="MTDVELKDEEKHRAKMAKRKAVQDAEVAEKTVEKGLLIVNTGPG
                     KGKTTAAFGLALRMLGYGKRVGVVQFIKGAWHTGEKDAFSAFGDRVAWHTMGEGFTWE
                     TQDLKRDIAAAEAAWAKALELMADPTISLVVLDEMNIALRYDYLDLDKVVAALKTRRE
                     GLHVVVTGRNAKPALVEAADLVTEMGSTKHHFSAGVKAQQGIEF"
     gene            complement(4066..>4573)
                     /locus_tag="VGM46_00365"
     CDS             complement(4066..>4573)
                     /locus_tag="VGM46_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HEY4191074.1"
                     /translation="SDGIVAPAFWLAVGGLTGGVAYKATNTADSMIGHRTPRHEAFGW
                     AAARFDDLINLPASRLTALLVVLGAFFVEGANPANAWRAIWRDAKKHRSPNAGWPEAA
                     MAGALGLALAGPRVYGGIPVDDLFMGEGGRREATSTDIRRALKLYWAADVLLIALFGA
                     LALSTILL"
BASE COUNT          843 a         1384 c         1490 g          856 t
ORIGIN      
        1 gccagcgccg ccacgaaggc cagcggcagg aaccatggca gggcaccgac gaaacagacg
       61 ccgatcaaca gcagcgtgtt ggcgagatag atcgggttgc gcgacaccgc aaacgggccg
      121 tttgtgacca gatgttccgg caagccgttc gaacaaagcg tcgtacgggt ggccgtcacg
      181 gcgcgaaacg cggtgaacca aagcactgct accgccagca gcaccagcca tccgatcgca
      241 aaaagcagat cgccgagcag tccgcctatc cagggaaggg gataagacca gccaagcaag
      301 gcggcgacgc cgattgccgc gagatagatc aatggcggcc atggcgcgcg cccagctacg
      361 gtccgggttg cggtcacggt gtttctcccc caactgccgc cttatcggtc tttgcggtac
      421 acaatccggc caggttgctg acatggtctt tcaattcctg gctctggtcg gcgccgtaga
      481 gccggtcgag cgtatcgtcg cgcgcgagca tgtcgaacat gcagccacaa taggtcgtgc
      541 agaaaggcgt gtcgcgggtg acttcgcacg aggcctggca tgatttgagg aagttttcct
      601 gccgggtttc gacatgcgcc ggcatgattt gcgaaaacgc ccagacgcag gcaatcagca
      661 ccggctgatg gatgaggtag aacgccaggc tgtgtttgcc gatgaagtta aggggtttgg
      721 cccatctgcc gggcgaaaac cggccaagcc atgcaagaac cccgaaagat cgcgccagtt
      781 ttgtcgccgc gatgccggcc agcaccgcac cgaaccacgg gaatagcggc acatagtcgt
      841 tggagtgcgg attgaccgtc gacaggccga cccaccacag ggagggatga tcgaagatcg
      901 gtgagcgcag gtagaaaggc agcgccacga ccgttgctgc gatcaccacc gtcagcagcg
      961 gcggcaacct caggaagagc aggccaagca ggcttgccag cgtgatttcg tgcaggatgc
     1021 cgaaaaagat aaagccatcc ggaaaggcgt atagcgtagc cgccgtgatc gccgccgccg
     1081 ccgccgccac catggccagg cgcttccaaa agcctttcca gcggatgccg gcgccatgtg
     1141 cgagcacgag gctgacgccg accaggaaca gaaaggtcga tgcgatgcag cgggcataaa
     1201 tcttccagcc gccaacgccg gtcaggcctg gctcggtata gccgaagaat tccaggtccc
     1261 aggtgaaatg gtagctcgcc atggccagca gggccgcgcc gcgcgcgacg tcgatggcgg
     1321 caatgcgtct tgaaggggca ggagaaggca cgtcgatgct catgcgggac tctgatcgct
     1381 tttgacgccc aaggattatc atgacagccg gccaggcagg aatggaaatg atgggttttc
     1441 cctgtccgcc accgcgccgg ccggcggccg cataattggc gtttggcggc aacggatttt
     1501 cccgcgagct ttgggccacg gccgtgaagc ggttggcaag gtgcgtattt cagcttggat
     1561 gagtcgtttc cctctgctgc gacaagcctc gaatcgtctc atcgtgttgc gcgggcgcat
     1621 tgtcgggcct ggagtagcag acgcccatgg gcacccatgt acgccatccg atgtggcttc
     1681 cttctgtgcg gcaggcgggc gcgcgcacgt tcgcttcgtt atacacggtc gaatcctttg
     1741 cccgcgccac cgtttccagc gtcatcccga tccaggctta cgaaatcctg cgcagcgagc
     1801 agagggtatc gatcctctat accgtcgttt cgctgctggg tttgtcggcc acgctgttca
     1861 tgccgatgct tattcatcgt ttcgcgcggc gctgggtcta caccgccgga gcttgcctgc
     1921 ttgccatcgg ttcgctgttt ttcctgaccg atacgctgcc cggccaggca atcggcatgc
     1981 tttgccgggt gatgggcgcc agcgccctct cgatcacgct caacctctac atcatggatc
     2041 acatccgcaa gaccgacttc atgcgttcgg aatcgctgcg catggcgtgg tccatggtcg
     2101 cctggaccgg aggcccgacg ctcggcatct tcctctacgc ccattatggc gtttatgccg
     2161 cgcatggcgc ggtcgccgcc tttgccatgc tgcttttagc gctgttctgg atttaccgcc
     2221 tgggcgacaa tccgctcatc gccgccggca agagccgcgc catcaatccg atcgccaata
     2281 tcggccggtt cgttgcgcag ccacgcctgc ggcttgcctg gatcatcgcc tttggacgct
     2341 cgtgcttctg gacgacgttt ttcgtctacg ggccgctgtt catggtcatc accggccaag
     2401 gcgaactggc gggcggcctg ctggtatcgg ccggaaacgg cctgttgttc atggccatct
     2461 actggggaag ggcgggcagg cggtatggcg cgcgccgcac catcaccctt gcatttttcg
     2521 ccatggcggc gacgctgctg gccgccggtg ccgtcggtga agccgtgccg ctgttgactg
     2581 cggcgttgct gcttggcggc gccctgttca ccatcgcgct ggacgcgctt ggctccccgg
     2641 tcttcatgcg ggcggtgcac ccttacgaac gcgcgcagat ggcggcggtc tatcgcacct
     2701 atctcgattt ttccgagctt acgccgccga tgatttattc cgtggtcctg gctttctttg
     2761 gcctcggctc ggttttcgcc acgcttggcg ttctggcgct gttttgcggc gccatctcct
     2821 ggcgatacct gccgaaagcc atgtagcccg ttgccggtcg ggccgatttt cctatagtcc
     2881 ggccatgggt gacaacggga agatgtgttg agaatgattg gtggctgctt tgcctgaccg
     2941 aagtctgacg tttttgatcg gtggcgcgcg ctccggcaaa agcgcgcatg ccgagcagtt
     3001 ggcgatgtgc catgcagcgc cgtggagtta tatcgccacc gcgcaggcct atgacgacga
     3061 gatgcgcgag cgcatcgcca cgcatcggtc gcgccggggg gagggatgga cgaccatcga
     3121 tgccccgctg gacctcgccg gcgcactcga tgccgtgccg gagggatgcc cggtgctggt
     3181 ggattgcctg acgctgtggc ttaccaacca catgctggcc gggcacgacc tgagcgccga
     3241 atgcgcacgg ctggagcaaa gcctgatgcg gccgcgcggt ccatggttcg tcgtatccaa
     3301 cgaggttgga ttggggatcg tgcccgaaaa tgcgctggcg cgccgtttcc gcgatgccgc
     3361 cgggcgtctc aaccagcagg tcgccgcggt ggccgatacg gtgctcttga tggtggccgg
     3421 gctgccgctg aaggtgaagt gatgacggat gtcgaattga aagacgaaga aaaacaccgc
     3481 gccaagatgg ccaagcgcaa ggcggtgcag gatgccgaag tggcggaaaa gaccgtcgaa
     3541 aaaggacttc tgatcgtcaa taccgggccg ggcaagggca agaccacggc ggctttcggg
     3601 ttggcgctga gaatgctcgg ctatggcaag cgtgtcggcg tcgtgcagtt catcaaggga
     3661 gcgtggcaca ccggcgagaa ggatgcgttt tcggctttcg gcgaccgcgt agcgtggcac
     3721 acgatgggcg agggctttac ctgggagacg caggatctga agcgggatat cgccgccgcc
     3781 gaagcggcct gggcgaaggc gctggaactg atggccgacc cgaccatcag ccttgtcgtt
     3841 ctcgatgaaa tgaacatcgc gttgcgctac gactatctcg atctcgacaa ggtggttgcc
     3901 gcattgaaga cacggcgcga aggtctgcat gtcgtcgtca ccgggcgaaa cgccaagccg
     3961 gcgctggtcg aagcggccga tctggtcacc gaaatgggtt cgaccaagca tcacttttcc
     4021 gccggcgtga aggcccagca gggcatcgaa ttctaggcaa tccgttcaaa gaaggattgt
     4081 cgaaagggcg agcgcgccga acagcgcgat aagcaggacg tcggcggccc aatagagctt
     4141 gagcgcgcgc cttatgtccg tgctggtggc ttcccggcga ccgccttcgc ccatgaacag
     4201 gtcatcgacc ggtatgccgc catagacgcg cgggccggcc aaggcgaggc cgagcgcgcc
     4261 ggccatcgcc gcttccggcc agccggcgtt gggcgagcga tgttttttgg cgtcgcgcca
     4321 tatggcgcgc caggcattgg ccggatttgc gccttcgacg aagaacgcgc cgagcacgac
     4381 cagaagcgcg gtcagccgcg atgccggcag gtttatcagg tcatcgaagc gtgccgccgc
     4441 ccagccgaac gcttcatggc ggggcgtgcg gtgaccgatc atggaatcgg ccgtgttggt
     4501 ggccttgtag gcgaccccac ccgtcaatcc gccaaccgcc agccagaaag ccggagcaac
     4561 gatgccgtca gag
//
LOCUS       DASYCS010000008         4530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4929764,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000008 DASYCS010000000
VERSION     DASYCS010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4530
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4929764"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1149)
                     /gene="glmM"
                     /locus_tag="VGM46_00370"
     CDS             complement(<1..1149)
                     /gene="glmM"
                     /locus_tag="VGM46_00370"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018430017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEY4191075.1"
                     /translation="MGRYFGTDGIRGRANKFPMTAEIAMRVGMAAGLSFQRGSHRHRV
                     VVGKDTRLSGYMIESALVSGLCAAGMDVFLLGPIPTPAVAMLVRSLRADIGVMISASH
                     NPYFDNGIKLFGPDGYKLSDEIETRIEAMLDNDIEMALADSDGLGRAKRVDGVHDRYI
                     EFAKRTLPRSMSLSGLRIVVDCANGAAYKVAPAVLWELGAEVITINDEPNGYNINNEC
                     GSTHPAGLQKKIHEVRADIGIALDGDADRVVIVDENGAIVDGDQIMALIAEAWHQNGR
                     LTGGGVVSTIMSNLGLERFLGGLNLQLHRTKVGDRYVVEHMRAHGLNVGGEQSGHIVL
                     SDFSTTGDGLVSALQVLACVKRQDKPVSELSKKFEPVPQLLKNVRFSGG"
     gene            1444..2484
                     /locus_tag="VGM46_00375"
     CDS             1444..2484
                     /locus_tag="VGM46_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006327731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191076.1"
                     /translation="MRIDRLATLTGLAVVTLFLASPASALTMAECSTKYNTAKDAGTL
                     GGQTWNQFRKAECAADASAATEEAKPAKKAAKASAKAADKTKAAPAAAAGDDAKGLTA
                     KECSAKYQAAKTAGTLNGMKWNDFRKSECAAGASAAVEEVAPAKKAKAAAADDAATGL
                     TSKECSTKYQAAKAAGTLNGMKWNDFRKSECAAGASAAVEDTQPTKAKASTKAADTAT
                     SGGAGLSMAECSTKYQAAKSGGTLNGMKWNDFRKASCGTGVSDDDTVPAADEASYTSE
                     PEAPKVKAPKGVKFPSSVSTKFSSETPGKARMHTCLEQYYANKDSDALGGLKWIQKGG
                     GFYSLCNARLKG"
     gene            complement(3133..>4530)
                     /gene="ftsH"
                     /locus_tag="VGM46_00380"
     CDS             complement(3133..>4530)
                     /gene="ftsH"
                     /locus_tag="VGM46_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009451015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEY4191077.1"
                     /translation="QKFQRLGGKIPRGVLLVGPPGTGKTLLARSVAGEANVPFFTISG
                     SDFVEMFVGVGASRVRDMFDQAKKNAPCIIFIDEIDAVGRHRGAGLGGGNDEREQTLN
                     QLLVEMDGFEANESIILIAATNRPDVLDPALLRPGRFDRQVVVPNPDIVGREKILKVH
                     VRNVPLAPNVDLKVIARGTPGFSGADLANLVNESALMAARRNKRLVTMAEFEDAKDKI
                     MMGAERRSSAMTQAEKELTAYHEAGHAILALNVPSADPLHKATIIPRGRALGMVMQLP
                     EGDRYSMSYKYMISRLAIMMGGRVAEEFKFGKENITSGASSDIEQATKLARAMVTRWG
                     FSDKLGHVAYGDNQEEVFLGHSVARTQNISEETAQIIDAEVRRLIDEAYNSARTILTK
                     QKKAWIALAQGLLEYETLSGEEIKQLIAGNKPSRDLGDDTPTSRGSAVPKAGATRGKK
                     KGPEPEGGMEPQPQG"
BASE COUNT          851 a         1379 c         1482 g          818 t
ORIGIN      
        1 gccgccggaa aagcgaacgt tcttgagcaa ttgcggcacc ggctcgaatt tcttcgaaag
       61 ttcgcttacc ggcttgtctt gccgcttgac gcaggccaac acctgcaagg ccgaaaccag
      121 gccgtcgccg gtggtggaga agtccgacaa cacgatatgg ccggactgct cgccgccgac
      181 attgaggccg tgggcgcgca tgtgctcgac gacataacgg tcgccgacct tggtgcggtg
      241 cagttgcagg ttaaggccgc cgaggaagcg ttccaggccg agattggaca tgatggtcga
      301 aacgacgccg ccaccggtca ggcggccatt ctggtgccag gcttcggcga tcagcgccat
      361 gatctggtcg ccgtcgacga tggcgccgtt ctcgtcgacg atgacgacgc ggtcggcatc
      421 gccatcgagc gcgatgccga tatcggcgcg gacttcatgg atcttctttt gcaggccggc
      481 cggatgggtc gagccgcatt cattattgat gttgtagcca ttcggctcgt cgttgatggt
      541 gatgacttcc gcgccgagtt cccaaagcac ggccggcgcc accttgtagg ctgcgccgtt
      601 cgcgcaatcg acgacgatcc gcaggccgga aagcgacatg gagcgcggca gggtgcgctt
      661 ggcgaattcg atatagcggt catggacgcc atcgacgcgc ttggcgcggc caaggccgtc
      721 ggaatcggca agcgccattt cgatatcgtt gtccagcatc gcttcgatgc gggtctcgat
      781 ctcgtcggaa agcttgtagc cgtccggccc gaacaacttg atgccattat cgaaataggg
      841 gttatgcgaa gccgagatca tcacgccgat atcggcgcgc agggaacgca ccagcatggc
      901 gacggcgggc gtcggaatag ggccgagcag gaacacgtcc atgccggcgg cgcacaggcc
      961 ggaaaccagc gcgctctcga tcatgtagcc ggaaagacgg gtatccttgc caacgacgac
     1021 ccggtgccgg tggctaccgc gctggaagga aagcccggcc gccatgccga cgcgcatcgc
     1081 gatttccgcg gtcatcggaa acttgttggc acgtccgcga atgccatcgg tgccgaagta
     1141 acgtcccatg cttgccggta tccctacctg tttccagctg gctctcttgc cacgtttgtc
     1201 gagacagtca tcatcacaat gtatgagtta ctgttacgat tccgttgttt tcggcgtgat
     1261 ttgcaggaac agcaagagaa atcaggttaa attgggcttt tgccggcatg agatgcggcc
     1321 gctgccatga tttgcgcgca atcgggcaat tctgatctgc gggtgcgact taatgtctgg
     1381 cacaacgggc cgcaaggcgg tattgcttgg cggctatttc gagcggcagc aatggagaaa
     1441 cgcatgcgca tcgatcgact tgctacattg accggccttg ccgtggtcac cctgtttctc
     1501 gcatcgccgg ccagcgcgct taccatggcc gagtgcagca ccaaatataa cacggccaag
     1561 gatgccggca cgctgggtgg ccagacgtgg aaccagttcc gcaaggccga atgcgccgcc
     1621 gacgccagcg ccgccaccga ggaggcaaag ccggccaaga aagcggccaa ggccagcgcc
     1681 aaggccgccg acaagaccaa ggcagcgcca gcggcggccg ccggcgacga cgccaagggc
     1741 ctgaccgcca aggaatgcag cgccaagtat caggcggcca agaccgccgg cacgctgaac
     1801 ggcatgaagt ggaacgactt ccgcaagagc gagtgcgcgg ccggggccag tgccgccgtc
     1861 gaggaagttg cgccagccaa gaaggcaaag gcggctgccg ccgatgacgc ggcaacgggg
     1921 ctgacctcca aggaatgctc gaccaaatac caggcggcca aggccgccgg caccttgaac
     1981 ggcatgaagt ggaacgactt ccgcaagagc gaatgcgcgg ccggcgccag tgccgccgtg
     2041 gaagacacgc agccaacgaa ggccaaggca tcgacaaagg ctgccgatac agcgaccagc
     2101 ggcggtgcgg ggctttcgat ggccgaatgc tcgaccaagt accaggctgc aaaaagtggc
     2161 ggcacgctga acggcatgaa gtggaatgac ttccgtaagg caagctgcgg cacgggcgtt
     2221 tccgacgacg acaccgtgcc cgccgccgac gaagccagct acaccagcga gccggaagcg
     2281 ccgaaagtga aggcaccgaa gggcgtcaag ttcccgtcct ccgtttccac caagttctct
     2341 tccgagaccc ccggcaaggc gcgcatgcac acctgcctgg agcagtatta tgccaacaag
     2401 gattcggatg cgcttggcgg cctgaagtgg attcagaagg gcggcggctt ctacagcctg
     2461 tgcaacgccc gcctgaaggg ctgatctttc gcccctgtta gtctttgaaa agcccgcgag
     2521 ccgaaaggtt cgcgggcttt ttgtccggtg gagccgccgc cctgcgtgcc ttgatggcgg
     2581 tttgcacgcc gttggggggc ggccggcgga cgcatatcgt cgcatagcgt gcaaacgacg
     2641 agatgccagc ctgcgcctgg gtggccgggt tccttttgtt cgttcgcggc caataggtca
     2701 ggcttagcgg aacggtaagc ctgacctatg tttgtcgaac gcaagcatgc tcggacctcc
     2761 gcgtaattcc acaaccgtgg gtgggcgcgg aggcggagcc gggcgtagcg cgtgacgcaa
     2821 tgccagcctg cccggggcgg cgggttcttc tgtccgctcc cggtcaatag gttaggcatg
     2881 gcgggacggt aagccgatcc agcgtttgtt gcgcgcaagc cgggacaccg gcgtaattac
     2941 gcaaccgttg caagcgcgga ggcgggcgtg tggcgggcag gccaacactt catgacgcgg
     3001 cctgccgcga agcgcggacc ggtagtgctt gggcctggcg tcaccttaaa gccgatgcct
     3061 gtgagcagct taaaaaagaa aacgccgcgc tggaaacgcg gcgttttccg gtaggtccgg
     3121 ttgctttccc gatcaccctt gcggctgcgg ctccatgccg ccttcaggct cggggccttt
     3181 ctttttgccg cgcgtggcgc cggccttcgg cacggccgag ccgcggctgg tcggcgtatc
     3241 gtcgccaagg tcgcgtgacg gcttgttgcc ggcgatcagc tgcttgatct cctcgcccga
     3301 cagcgtctca tattccagca gcccctgcgc cagcgcgatc caggccttct tctgcttggt
     3361 caggatggtg cgggccgagt tgtaggcttc gtcgatcagg cggcgcactt cggcgtcgat
     3421 gatctgcgcg gtttcctccg agatattctg cgtgcgcgcc accgaatggc cgaggaaaac
     3481 ctcttcctgg ttgtcgccat aggcgacgtg gccgagcttg tcggaaaagc cccagcgcgt
     3541 aaccatggcg cgcgccagtt tggtcgcttg ctcgatgtcg gaagaggcgc cggaggtgat
     3601 gttttccttg ccgaacttga actcttccgc cacgcgcccg cccatcatga tggcgaggcg
     3661 cgagatcatg tatttgtagc tcatcgaata gcggtcgcct tccggcaact gcatgaccat
     3721 gccgagcgcg cggccgcgcg gaatgatcgt cgccttgtgc agcggatcgg cggacggcac
     3781 gttcagcgcc agaatggcgt ggccggcctc gtgataggcg gtcagttcct tctccgcctg
     3841 cgtcatggct gaggagcggc gctccgcgcc catcatgatc ttgtctttgg catcctcgaa
     3901 ttcggccatg gtgacgagac gcttgttgcg ccttgccgcc atcaaggcgc tttcgttgac
     3961 gaggttggcg agatcggcgc cggagaaacc gggcgttccg cgcgcgatga ccttgagatc
     4021 gacattgggc gccagcggca cgttgcgcac atgcactttc aggatttttt cgcggccgac
     4081 gatatcgggg ttcggcacca cgacctgccg atcgaaacgg cccggacgca aaagcgccgg
     4141 gtcgagcacg tcgggacggt tggtggcggc gatcaggatg atcgattcat tggcctcaaa
     4201 gccatccatc tcgaccagca actggttcag cgtctgctcg cgctcgtcat tgccgccgcc
     4261 gagaccggcg ccacgatgac ggccgaccgc gtcgatttcg tcgatgaaga tgatgcaggg
     4321 cgcgttcttc ttggcctggt cgaacatgtc gcggacgcgg gaagcgccga cgccgacgaa
     4381 catctcgacg aagtccgagc cggaaatggt gaagaagggc acattggcct cgcccgccac
     4441 ggagcgcgcc aagagcgtct tgccggtgcc gggagggccg acgagcagca cgccgcgcgg
     4501 aatcttgccg ccgaggcgct ggaacttctg
//
LOCUS       DASYCS010000009         3916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2465738,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000009 DASYCS010000000
VERSION     DASYCS010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2465738"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="VGM46_00385"
     CDS             complement(<1..740)
                     /locus_tag="VGM46_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY4191078.1"
                     /translation="MATERYNPRAAEPKWQKAWDAKKLFETRNDDPRPKYYVLEMFPY
                     PSGRIHMGHVRNYTMGDVVARYHRAKGENVLHPMGWDAFGLPAENAARDNHVNPRDWT
                     YANIETMKGQLKTMGLSLDWSREIATCNPAYYRHQQKMFLDFWRAGLAERKSAKVNWD
                     PVDMTVLANEQVIDGKGWRSGAAVEQRDLTQWFFKITAMAQDLLDSLATLDRWPEKVR
                     VMQENWIGRSQGLLMRWPLTPDTAPAGA"
     gene            894..1556
                     /locus_tag="VGM46_00390"
     CDS             894..1556
                     /locus_tag="VGM46_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEY4191079.1"
                     /translation="MGDAVEQLAGVKAKIAAAQSAAKRDNGAVTLVAVSKTFEADAVR
                     PVLEAGQRVFGENRVQEAQGKWPALKADFPDIELHLIGPLQSNKAKEAVALFDAIETV
                     DREKIAAELAREMERQGRTLKLYVQVNTGSEPQKAGIEPREAVAFVKRCRDVHKLAIE
                     GLMCIPPAEENPGPHFALLEKLAREAGVEKLSMGMSGDYETAVAFGATSVRVGSAIFG
                     SR"
     gene            complement(1612..2718)
                     /locus_tag="VGM46_00395"
     CDS             complement(1612..2718)
                     /locus_tag="VGM46_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013636629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY4191080.1"
                     /translation="MKSRLLAGVTLAASFAFASMAHADMVIGLVAPLTGPVAAYGDQV
                     KNGAQAAVDTINKAGGILGEQVTLKLADDAGDPKQGVSAANQLVGDGVHYVVGPVTSG
                     VAIPASDVFAENGVLMVTPTATAPDLTTRGLTNVLRTCGRDDQQAEVSANYVLAHLKD
                     KKVAIIHDKGTYGMGLANAFKAAINKGGVTEVMFEGLTPGEKDYSSLVGRLKKDGAEV
                     IYFGGYHPEGGLLARQLADVQVKAQLIGGEGLSNTEYWSIGNEAAAGTLFTNATDALK
                     NPDSAEAVAVLKEKGIPPEAFTLNAYAAVQVIKAGIEKAGKADDPAAVATALKGGEAV
                     QTAIGKLTYGENGDLSSPSFSLFKWEDGKIVAAE"
     gene            complement(2878..3150)
                     /locus_tag="VGM46_00400"
     CDS             complement(2878..3150)
                     /locus_tag="VGM46_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEY4191081.1"
                     /translation="MRDEPELNLMLNGYGLTTANILYHLPDHPHLLQSFVWQEYDIAP
                     RFPVLVKFIEFWKQKLDGPLHSITYTHRKLIAPNEWRKVDGEFLLH"
     gene            3429..>3916
                     /locus_tag="VGM46_00405"
     CDS             3429..>3916
                     /locus_tag="VGM46_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY4191082.1"
                     /translation="MSEVHVHKVQPAWKKDALIDNETYLKWYADSVKNPDKFWGKHGK
                     RIDWFKPYTKVRNASFDGKVSIKWFEDGTTNVSYNCIDRHLKKRGKQTAIIWEGDNPY
                     DEKKVTYNELHEHVCRFANVLKKHGVKKGDRVTIYMPMIPEAAYAMFACTRIGAIHSV
                     VFG"
BASE COUNT          728 a         1131 c         1281 g          776 t
ORIGIN      
        1 ctcgcgccgg ccggcgcggt gtccggggtc agcggccagc gcatcagcag gccctgcgaa
       61 cggccgatcc agttttcctg catgacgcgg accttttccg gccagcggtc gagcgtggcg
      121 aggctgtcca gcaaatcctg cgccatggcg gtgatcttga agaaccactg cgtcaggtcg
      181 cgctgctcga cggccgcgcc ggaacgccag cccttgccgt cgatgacctg ctcgttggcc
      241 agtaccgtca tgtcgaccgg gtcccagttg accttggcgg atttgcgctc ggccaggccg
      301 gcgcgccaga aatcgaggaa catcttctgc tggtggcggt agtaggccgg attgcaggtg
      361 gcgatctcgc gcgaccagtc gagcgacagg cccatcgtct tcaactggcc tttcatcgtc
      421 tcgatattgg cataggtcca gtcgcgcgga ttgacatgat tgtcgcgcgc ggcgttttcc
      481 gccggcaggc cgaaggcgtc ccagcccatc gggtgcagca cgttttcacc cttggcgcgg
      541 tgatagcgcg caaccacgtc gcccatcgta tagttgcgga catggcccat gtggatgcgg
      601 cccgacggat agggaaacat ttccagcaca tagtatttcg ggcgcgggtc gtcgttgcgg
      661 gtttcgaaca gctttttggc gtcccaggct ttctgccatt tgggctcggc cgcgcgcggg
      721 ttgtatcgtt cggttgccat gatccgtttt tcacttaaag aaggcgggga aaacccggga
      781 ttgcatcttc ataagaccgt ggttcgggtc ttcaccacgg tttgacaaac ccgtcaacca
      841 tatgggtgcc cgcatatgag tgggctaggg cacgaaaacg gagaaattga accatgggcg
      901 atgcggtgga gcagcttgcc ggggtcaagg cgaagatcgc ggcggcgcag agcgcggcga
      961 aacgcgacaa tggcgcggtt acgctggtgg cggtgtccaa gacgttcgag gccgacgcgg
     1021 ttcgcccggt gctggaagcc gggcagcgcg tgttcggcga aaaccgcgtt caggaagcgc
     1081 aaggcaaatg gccggcgctg aaggcggatt ttccggatat cgaactgcat ctgatcggcc
     1141 cgctgcaatc caacaaggcc aaggaagccg tcgccctgtt cgacgcaatc gagacggtcg
     1201 accgcgagaa gatcgcggcg gaactggcca gggaaatgga gcggcagggg cgtacgctta
     1261 aactctacgt gcaggtcaat accgggtccg agccgcagaa agccggcatc gagccgcgtg
     1321 aggcggtggc cttcgtcaag cgctgccgcg acgttcacaa gctggcgatc gaagggctga
     1381 tgtgcattcc gccggcggaa gaaaatcccg gcccgcattt cgcgctgctg gaaaagctgg
     1441 cgcgcgaagc cggggtggaa aaactgtcga tgggcatgtc cggcgactat gagacggcgg
     1501 ttgccttcgg cgcaaccagt gtgcgcgtcg gctcggccat cttcggttcg cgctgatttt
     1561 tgaaaagaaa aggccgggtt gccccggcct tttgtttttg tctggcgaaa attattcggc
     1621 ggcgacgatc ttgccgtctt cccatttgaa cagcgagaag cttggcgagg aaagatcgcc
     1681 gttctcgcca taggtcagct tgccgatggc ggtctggacc gcttcaccgc ccttgagcgc
     1741 ggtggcgacg gctgccggat cgtcggcctt gccggctttt tcgatgccgg ccttgataac
     1801 ctggacggcg gcataggcgt tgagcgtgaa ggcttccggc ggaatgccct tttccttgag
     1861 gacggccacg gcctcggccg aatccgggtt cttcaaggcg tcggtggcgt tggtgaacag
     1921 cgtgccggcg gcggcttcgt tgccgatcga ccagtattcg gtgttcgaca ggccttcgcc
     1981 gccgataagc tgcgccttga cctgcacgtc agccagctga cgggccagaa ggccgccttc
     2041 ggggtggtag ccgccgaaat agatgacctc ggcgccgtcc ttcttcaggc ggccgacgag
     2101 cgaggagtaa tccttttcgc ccggcgtcag gccctcgaac atcacttcgg tgacgccgcc
     2161 cttgttgatt gccgccttga acgcattggc aaggcccatg ccgtaggtgc ccttgtcgtg
     2221 gatgatggcg actttcttgt ccttcaggtg ggcgaggacg tagttggcag agacttccgc
     2281 ctgttggtcg tcgcgcccgc aggtgcgcag aacattggtc aggccgcgcg tggtaaggtc
     2341 gggtgcggtg gcggtcggcg tcaccatcag gacgccgttt tccgcaaaca catcggaggc
     2401 ggggatggcg acgccggacg tcacggggcc gacgacgtaa tgcacgccat cgccgacgag
     2461 ctggttggcg gcggacacgc cctgcttggg gtcgcccgca tcgtcggcaa gcttgagtgt
     2521 cacctgctcg cccagaatgc ctcctgcctt gttgatcgta tcgacggcag cctgcgcgcc
     2581 gttcttcacc tggtcgccat aggcggcgac cgggccggtc agcggcgcga ccaggccgat
     2641 gaccatgtcg gcatgcgcca tcgatgcgaa tgcgaatgaa gcggccagcg tcacgccggc
     2701 caacagacgg gatttcatgg gctttctcct tgtgcgacag ccaatgcggc ctgtcgaagc
     2761 caagcggatt caatattcct gcgaggccgg ctttgcaacc agatcaacca tttgaaaaca
     2821 ggcgattgct tgggccagcg gcactgcccc gccgactatg tcccaatgaa ataggtgtca
     2881 gtgcaatagg aattcgccgt cgaccttgcg ccattcgttg ggcgcgatca gcttgcggtg
     2941 cgtgtaggtg atcgaatgaa gcgggccgtc gagcttctgc ttccagaatt cgatgaattt
     3001 gaccagcacc ggaaagcgcg gcgcgatgtc gtattcctgc cagacgaagc tttgcagcag
     3061 atgcggatgg tccggcaggt ggtagaggat gttggcggtc gtcaggccgt agccgttcag
     3121 catcaggttc agttcaggtt catccctcat ggttgccctc gcaagcgttg agtgcctgtc
     3181 tcccggcctg gattctgcgc cgggatggtt aatcaactat tgaaatgcgc cggctgcggc
     3241 ccggctcatg gcgttcgccg gcgcacacgc catcgaggcg ggcagctgcg tcatcacgcc
     3301 gctgcccggc aaaggtctga tgttgccgtc gcggcggaag tagtaataat gccatgcgcg
     3361 gcggcctttt tttgccgcgc tgccgtcagc gcaagcgccg gcaatcgatt tcgggaggaa
     3421 agcagaatat gtcagaggtt cacgtccaca aggtgcagcc ggcgtggaag aaagatgcgc
     3481 tgatcgacaa tgagacgtat ctgaaatggt acgccgacag cgtgaaaaat cctgacaagt
     3541 tctggggcaa gcacggcaag cggatcgact ggttcaagcc ttataccaag gtcaggaacg
     3601 cttccttcga cggcaaggtc tcgatcaagt ggttcgagga cggcaccacc aatgtttcct
     3661 acaattgcat cgaccgtcac ctgaagaagc gcggcaagca gacggcgatc atctgggaag
     3721 gcgacaatcc ctatgacgag aagaaggtca cctataacga gctgcacgag catgtctgcc
     3781 gcttcgccaa tgtgctgaag aagcacggcg tcaagaaggg cgaccgcgtc accatctaca
     3841 tgccgatgat cccggaagcg gcctatgcga tgtttgcctg cacgcgcatc ggggcgatcc
     3901 attccgtggt gttcgg
//
LOCUS       DASYCS010000010         7277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1596476,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000010 DASYCS010000000
VERSION     DASYCS010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 7277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7277
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1596476"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VGM46_00410"
     CDS             <1..288
                     /locus_tag="VGM46_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HEY4191083.1"
                     /translation="LGLNVAGKVLVMPSGRGSSSSSSVLAEAIRCGTAPAAILIERPD
                     PILAVGAIVAEFLYAIRMPLVVCDIAGIVSGDPISIRSNAAGQAVVTLPAA"
     gene            complement(292..1428)
                     /locus_tag="VGM46_00415"
     CDS             complement(292..1428)
                     /locus_tag="VGM46_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018688654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY4191084.1"
                     /translation="MTALPDFKLETYFSKWEFTARYHMTASDAQSMTMDELLAMGTDA
                     QREAFRTQWLGYTETFGKPDLRRTIAGLYDHMESEDILCFAGAGEGIYIAMHVLLGKG
                     DHALVVTPNYQSAETVPLSICEVSGVPLDPADNWSLDIDAVRAAIRPNTRLISVNFPH
                     NPTGKVLERDRFDALVELCREHGIWLFSDEVYRGLSVEGRHQLPAAADAYERGLSLAV
                     TSKAYGLPGLRIGWIACRDRAVLSRMERMKHYISICNSAPSEALAQIALENAPRILAR
                     NSRLVAENLSLLRQLFADFDGLFEWYSPDGGCVGYPRYNGKEGVEAFCRDLVERSGVL
                     LLPASIYQSELNPTPDGRFRIGFGRANIEEGLAALRTHLMRNAA"
     gene            complement(1698..2312)
                     /locus_tag="VGM46_00420"
     CDS             complement(1698..2312)
                     /locus_tag="VGM46_00420"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015314176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEY4191085.1"
                     /translation="MRIKSLLVAAALATTLAGCASIGPMQVFTNDYGAVTDAGYQLPR
                     IPIEKVPMKYRRQIVRYETGEKPGTIIVDTSNKFLYFVMGDGKAMRYGIGVGREGMEW
                     SGTAHIALKREWPTWTPPAAMIGRQPELKKWAHGMPPGLTNPLGARALYLFNKGGDSG
                     YRLHGSPEWNSIGHAMSSGCIRLMNQDIMDLYNRAEVGAKVIVM"
     gene            2529..2921
                     /gene="cueR"
                     /locus_tag="VGM46_00425"
     CDS             2529..2921
                     /gene="cueR"
                     /locus_tag="VGM46_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006878 - intracellular copper ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HEY4191086.1"
                     /translation="MNVGDAARRAGLPAKTIRYYEEIGLIQPARAGNGYRSYSGQDIH
                     RLAFLKRARTLGFSIDDCRQLMALYQDRSRASADVRQIAAAHVATIEEKLRELQAMRA
                     TLQELVHACHGDERPDCPILEEMAGAAQ"
     gene            complement(2961..3311)
                     /locus_tag="VGM46_00430"
     CDS             complement(2961..3311)
                     /locus_tag="VGM46_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015317692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HEY4191087.1"
                     /translation="MCMVFAVQDPEGYRQINRSIRIDGHSTSIQLESTFWILLDEIAE
                     SQDLTTPKFISKLYDEAIEINGEIPNFASMLRTTCALYLRGLRPGLETPLAHKREANV
                     GMLTLHNEASVPRG"
     gene            3552..3947
                     /locus_tag="VGM46_00435"
     CDS             3552..3947
                     /locus_tag="VGM46_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY4191088.1"
                     /translation="MAKAIHTMVRVLDEARSVAFYRNAFGLSVAERLDFETFTLVYLS
                     NADAEFEVELTVNKDRVEPYVLGDGYGHLAVSVADLDGEHDRMGALGLNPKNIVEFKR
                     DGVLIARFFFIEDPDGYKIEVLQRQGRFK"
     gene            4014..4562
                     /locus_tag="VGM46_00440"
     CDS             4014..4562
                     /locus_tag="VGM46_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019858314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HEY4191089.1"
                     /translation="MVTIYDGRPKSSDKAGLSRRELLKRGGVGALLVISGSAVISPEH
                     AWGLEASALKPETMATLIQMARDIYPHDQVADKYYAIAVKGHDELAGKDAAHKELIEK
                     GVADLNAKAGKDGYSALGWEDERVAVLKQIETTPFFQAVRGGLVVGLYNQKEIWPIFG
                     YEGESYSKGGYISRGFDDIEWL"
     gene            4617..6188
                     /locus_tag="VGM46_00445"
     CDS             4617..6188
                     /locus_tag="VGM46_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015317695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEY4191090.1"
                     /translation="MAAPFDLKNKDVVVIIGSGAGGGTLGNELAQKGIDVVILEAGAR
                     HEYEDFINDEWDSFAQLAWKDKRIASGDWRVAKDFPNLPAWIVKAVGGSTVHWAGASL
                     RFQEHEFKTLTTYGKMEGANLLDWPITLAEMEPYYARAEDKMGVTRTNGIPGLPGNNN
                     FKVLKAGADKLGYKECHTGNMAINSEPRDDRNSCQQTGFCFQGCKWGAKWSTLYTEIP
                     KGEATGHLEVRPNSMAVKINHDASGKVTGVVYADKDGKLQEQKARIVAVAGNSIESPR
                     LLLNSNSSKFPDGLANSSGQVGKNYMRHTTGSVYAIFDKPVHMYRGTTMAGIVRDEAR
                     HDPSRGFSGGYELETVSIGVPFMAAFLDPGAWGRSFTTAIDHYDHMAGLWIVGEDMAR
                     EENRITLHTEEKDEHGMPIADVHFDDHPNDTAMRNHAYKQSIALYDAVGATRTLPTPP
                     YPSTHNLGTNRMSEKAKDGVVNKHGQAHDVSNLFVSDGSQFTSGGAENPTLTIVSLAI
                     RQAEYIADQMKAKTI"
     gene            complement(6425..>7277)
                     /locus_tag="VGM46_00450"
     CDS             complement(6425..>7277)
                     /locus_tag="VGM46_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018067377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HEY4191091.1"
                     /translation="QQETEAWMMRERPQVVFLAAAKVGGIHANDRRPADFIYDNLMIE
                     ANVIHAAFLAGVEKLVLLGSTCIYPRLAPQPIPEEALLTGPLEPTNEWYAVAKIAGIK
                     LAQAYRRQHGCDFISAQPTNLYGPGDNFDLETSHVLPALMRKAHDAKQEGAASMTVWG
                     SGTPLREFLHVDDLADALVFLAQHYSGEGPVNVGSGVEISIRDLADAIRKAVGFEGQL
                     MFDAAKPDGTPRKLADISKLAAMGWRHRIGLDEGLASTYDWFLNHAAAVHKPADERLT
                     PLLRSRS"
BASE COUNT         1437 a         2261 c         2216 g         1363 t
ORIGIN      
        1 cttgggctaa acgtcgccgg caaggtgctg gtcatgccga gcgggcgagg ttcctcgtcc
       61 tcttcctcgg tactggccga ggcgatccgt tgcggcacgg cgccggcggc cattctcatc
      121 gagcggcccg atcccatcct ggcagtcggc gccatcgtcg ccgaatttct ctatgccatc
      181 cgcatgccgc tggtggtctg cgacattgcg ggcattgtct ccggcgatcc catatcgatc
      241 cgctcaaacg ctgccggcca ggccgttgta acgctgccgg cggcctagct tctaggccgc
      301 gttccgcatc agatgggtgc gcagagcggc caggccttct tcgatgttgg cgcgcccgaa
      361 accgatgcgg aaacgcccgt ccggcgtcgg gttgagttcc gattggtaga tgcttgccgg
      421 cagcagcaaa acgccggagc gctcgaccag atcgcggcag aaggcctcga cgccttcctt
      481 gccattgtag cgcggataac cgacgcaacc gccatcgggg ctgtaccatt cgaacaatcc
      541 atcgaaatcg gcaaacaact gcctcagaag cgacaggttt tccgccacca gccggctgtt
      601 gcgcgccagg attcgcggcg cgttttccag cgcgatctgg gccagcgctt cgctgggcgc
      661 ggaattgcag atggaaatat aatgcttcat gcgttccatg cgcgacagca cggcgcggtc
      721 gcggcaggcg atccagccga tgcgcaggcc gggcaggcca taggctttcg aggtcaccgc
      781 aagcgacagg ccgcgctcat aggcgtcggc ggccgccgga agctggtggc ggccttcgac
      841 gctcaatcca cgatagacct catcgctgaa aagccagatg ccatgttcgc ggcataattc
      901 aaccagcgcg tcgaaacggt cccgttccag aacctttccg gtcggattgt gcggaaaatt
      961 gaccgaaatc agcctggtat tcggccggat cgcggcacgg acggcatcga tgtcgagcga
     1021 ccagttgtcg gcgggatcga gcggcacgcc ggaaacctcg cagatgctca atggcacggt
     1081 ttcggccgat tgatagttgg gcgtcaccac cagcgcgtgg tctccctttc ccagcaggac
     1141 atgcatggcg atgtagatgc cttcacccgc cccggcgaag cacagtatgt cctcgctttc
     1201 catgtggtcg tagaggccgg caatggtgcg gcgcaaatcc ggcttgccga aggtttccgt
     1261 atagccgagc cattgcgtcc ggaacgcttc gcgctgggca tcggtcccca tggcgagcag
     1321 ctcgtccatc gtcatgctct gggcgtccga cgccgtcatg tggtagcggg cggtgaactc
     1381 ccatttcgag aagtaggttt caagcttgaa atcgggcagg gcggtcatcg gcggcctctc
     1441 ggttgcgcgg ggcgtcttgt tgacgcttat tgggccaagg gccatgccaa ggcttcagcg
     1501 cagcccggcc aatgccgttg taccagaggc gatcaccacc ggcgatacgg catcgacgcc
     1561 ggctggccaa aggcggaggc cccagtccct gccgcgtgat ggttgacaga taacccgcct
     1621 caaaacaaaa aaccgggcca aaagcccggt ttcctgcgta ggtcgcccgt tgttgcctgg
     1681 acggtccgcg tatcgattta cattacgatg accttggcgc ccacttcggc gcggttgtag
     1741 aggtccatga tgtcctggtt catcaggcgg atgcagcccg acgacatggc atggccgatc
     1801 gagttccatt ccgggctgcc atgcagccgg tagccggaat cgccgccctt gttgaacagg
     1861 tagagcgcgc gcgccccgag cggattggtc aggcccggag gcatgccgtg cgcccatttc
     1921 ttcagttcgg gctggcgtcc gatcatcgcc gccggcggtg tccaggtcgg ccattcgcgc
     1981 ttcagcgcga tatgggcggt gccgctccac tccatgcctt cgcggccgac gccgatgccg
     2041 tagcgcatcg ccttgccatc gcccatgacg aaatagagga acttgttgga cgtatcgacg
     2101 atgatggtgc cgggcttttc gccggtttcg tagcgcacga tctggcggcg atatttcatc
     2161 ggcacctttt cgatcggaat gcgcggcagc tgatagccgg catcggtcac cgcgccgtaa
     2221 tcattggtga acacctgcat cggaccgatg ctggcgcaac ccgcaagcgt ggttgccagc
     2281 gccgccgcga cgagaagaga cttgatgcgc atgaacagtt gccccgccaa tcctggtggc
     2341 tcgaatcgag ccttgttttc gccgtcattc atgcgggcat ttcttttggc tgccaagcag
     2401 cgggtgctta tatgcaaggc gtgccgttgc cttaagctgc attctgtagc atttcggcaa
     2461 caatgctcac ggaaatgtga agtaaattca atgatctgaa acggtctggc ctacggaagg
     2521 aaacaaccat gaatgtcggt gacgccgccc gccgcgccgg gcttccggcc aagaccatcc
     2581 gttactatga ggaaatcggc ctgatccagc cggcgcgcgc cggcaatggc taccgctctt
     2641 attcaggcca ggacatacac cgcctggcct tcctgaagcg cgcgcgaacc ttgggctttt
     2701 ccatcgacga ttgccgccag ctgatggcgc tttaccagga ccgaagccgc gccagcgccg
     2761 atgttcgcca gatcgccgcc gcccatgtcg ccactatcga ggaaaagctg cgcgaattgc
     2821 aggcgatgcg ggcgacgctg caagaactcg tccatgcctg ccatggcgac gagcgcccgg
     2881 attgcccgat cctggaagaa atggcgggcg ccgcccaata gcgaagccgc gcccgaacct
     2941 atacgcgtcg gcctgtccgc tcagccccga ggcacgctgg cctcgttatg cagcgtcagc
     3001 atgccgacat tggcttcccg cttgtgcgcc agcggggttt ccaggccggg gcgcagcccg
     3061 cgcaggtaaa gcgcgcaggt ggtgcgcagc atcgaggcga aattcggtat ctcgccattg
     3121 atctcgatcg cctcgtcata aagcttcgaa atgaactttg gcgtggtcaa atcctggctt
     3181 tcggctattt cgtccagcag gatccagaat gtcgattcaa gctggatgct ggtcgaatga
     3241 ccgtcgatgc ggatcgagcg attgatctgg cggtagcctt ccggatcctg aacggcaaaa
     3301 accatacaca tttctgaaac ctcccgactg ctttcggcgc ggcgaaagga atatttctcc
     3361 cgtcggcgcg ccacttctcc cgtcggcata ttccattcaa aaaagcgaaa cggcaacagg
     3421 actttcgtct ctatcaccaa gcataaaatc atgcgtggta accggctgcc accatctttg
     3481 cccggcgcca cccataatcc tgttgaagcg ggtcctgaat caggtcctga agcgggtggg
     3541 tggagttcaa tttggcaaag gcgatccata ccatggtccg ggtgctcgat gaggcccgct
     3601 cggtggcgtt ttatcgaaat gcgttcggtc tttcagtggc ggagcggctg gatttcgaaa
     3661 ccttcacgct tgtctatctc tccaacgccg atgcggaatt cgaggtcgag ctgaccgtca
     3721 acaaggaccg cgtcgagcct tatgtcttgg gcgacggcta cggccacctg gcggtttcgg
     3781 tggccgatct cgacggcgag cacgaccgca tgggcgctct cggtctcaat cccaagaaca
     3841 ttgtcgagtt caagcgggat ggtgtcctga tcgcgcgctt cttcttcatc gaagatcccg
     3901 acggctacaa gatcgaggtc ctgcaacggc agggacggtt caaataggag gatgctcgcc
     3961 agccgagcca gccacggcac ctgacgtgcc caaagcaagc caaggaggaa aaaatggtaa
     4021 cgatttacga cggaaggccg aagtcttcgg acaaagcggg gctttcgcgg cgagagcttc
     4081 tcaagcgcgg cggcgtcggc gcgctgctgg tgatttccgg cagcgccgtc atcagccccg
     4141 aacacgcatg gggccttgaa gcatcggccc tcaagccgga aaccatggcg acgctgatcc
     4201 agatggcgcg cgatatctat ccgcacgatc aggttgcgga caaatattac gccatcgccg
     4261 tcaagggcca tgacgagctg gccggcaagg acgccgccca caaggaactc atcgaaaaag
     4321 gcgtcgccga tctgaacgcg aaggccggca aggacggcta cagcgcgctt ggctgggagg
     4381 atgagcgcgt cgccgtgctc aagcagatcg agacgacgcc gttcttccag gcggtgcgcg
     4441 gcgggttggt cgtcggcctc tacaaccaga aggagatctg gccgatcttc ggttatgagg
     4501 gcgagtccta ttcgaagggc ggctacatct cgcgcgggtt cgacgacatc gagtggctct
     4561 aggccccatc cgtcgcccca ccgcatcaca ccattcgcac atcatgggag gttaccatgg
     4621 cagcaccatt tgatctgaag aacaaggacg tcgtcgtcat tatcggctca ggcgccggcg
     4681 gcggcacgct gggcaacgag ctggcgcaaa aaggcatcga tgtcgtcatc ctcgaagccg
     4741 gcgcgcgcca cgaatatgag gatttcatca acgacgagtg ggattccttc gcccagctcg
     4801 cctggaagga taaacgcatc gcatccggcg actggcgcgt cgccaaggac ttccccaatt
     4861 tgccggcatg gatcgtcaag gcggtcggcg gctccaccgt ccattgggcc ggcgcctcgc
     4921 ttcgcttcca ggagcatgag ttcaagacgc tcaccaccta tggcaagatg gaaggcgcca
     4981 acctgctcga ctggccgata acgctggctg aaatggagcc ttattacgcc agggccgagg
     5041 acaagatggg tgtgacccgc accaacggca ttcccggcct gcccggcaac aacaatttca
     5101 aggtgttgaa ggccggcgcc gacaagctcg gctacaagga atgccatacc ggcaacatgg
     5161 ccatcaattc cgagccgcgc gacgaccgca attcctgcca gcaaaccggc ttctgcttcc
     5221 agggctgcaa atggggcgcc aagtggtcga cgctctatac tgaaattccc aagggcgagg
     5281 cgaccgggca tcttgaagtg cggccgaatt ccatggccgt caagatcaac cacgacgcca
     5341 gcggcaaggt caccggcgtg gtttatgccg acaaggacgg caagttgcag gaacagaagg
     5401 cgcgcatcgt cgccgtggcc ggcaactcga tcgaaagccc gcgcctgctg ctcaattcca
     5461 attcgagcaa gttcccggac gggctggcca attcgtccgg ccaggtcggc aagaactaca
     5521 tgcgccacac caccggttcg gtctacgcca tcttcgacaa gccggtgcat atgtatcgcg
     5581 gcaccaccat ggccggcatc gtccgcgacg aggcgcgcca cgatccgtcg cgcggcttct
     5641 cgggcggcta cgagttggaa acggtttcca tcggcgtgcc cttcatggcg gccttcctcg
     5701 atccgggcgc ctggggccgc tccttcacca cggcgatcga ccattacgac cacatggccg
     5761 gcctgtggat tgtcggcgag gacatggcgc gcgaggaaaa ccgcatcacg ctgcatacgg
     5821 aagaaaagga cgagcacggc atgccgatcg ccgatgtcca tttcgacgac caccccaacg
     5881 acaccgccat gcgcaaccat gcctacaagc agtcgatcgc gctctacgat gcggttggcg
     5941 ccacgcgcac tctgccgacc ccgccctatc cgtcgacgca taatctcggc accaaccgga
     6001 tgagcgagaa ggccaaggat ggcgtcgtca ataagcacgg gcaggcgcac gacgtttcaa
     6061 acctgttcgt gtcggacggc agccagttca cctccggggg cgcggaaaat ccaaccttga
     6121 cgatcgtcag cctggctatc cggcaggccg aatatatcgc cgaccagatg aaggcgaaaa
     6181 ccatctgatt ccggcgtatg ccgggtcctg aaccccggca gtgaagctgg cgcaattccg
     6241 gggaaggaaa tcggtttccc ggaattgcgt gaacaaagat tggagcggtt cagcctttca
     6301 aggcatgaag cgctccaatt gtagtccctg gaaaaatgcc ggacaaccgc atccgaaacc
     6361 attcgggcgc ggcgcgcggc cacgctctgt cagggggcgt tcccattttc ctcgtcgctc
     6421 tgcgtcagga cctcgaccgc agcagcggcg tcaagcgttc atcagcgggc ttatgtactg
     6481 ccgcagcgtg gttgaggaac cagtcatagg tcgatgcgag gccttcatcg aggccgatgc
     6541 gatggcgcca gcccatcgcg gcaagcttgg aaatatccgc cagcttgcgc ggcgtgccat
     6601 cgggcttggc ggcgtcgaac atcagctgcc cctcgaagcc aaccgctttg cggatggcgt
     6661 cggcaagatc gcggatcgaa atctccacgc ccgacccgac attgaccggc ccctcgccgc
     6721 tgtaatgttg cgcgaggaaa accagtgcgt cggcaaggtc gtcgacatgc aagaattcgc
     6781 gcaacggcgt gccggacccc cacacggtca tgcttgcggc gccttcctgt ttcgcgtcat
     6841 gcgccttgcg catcagcgcc ggcaggacgt ggctggtttc gagatcgaaa ttgtcgcccg
     6901 gaccgtagag gttggtcggt tgcgctgaaa tgaagtcgca gccatgctgc cgccggtagg
     6961 cctgggcaag cttgatgccg gcgatcttgg ccaccgcata ccattcgttg gtcggctcaa
     7021 gaggaccggt cagcagcgcc tcctcgggga tcggctgtgg cgccaggcgc ggatagatgc
     7081 aggtggaacc cagcaacacc agcttctcca cgccggcaag gaacgccgca tgaatcacat
     7141 tggcctcgat catcaaattg tcgtagatga aatcggcggg gcggcggtca ttggcatgga
     7201 tgccgccgac cttcgcggcg gcgagaaaca ccacctgcgg tctttcccgc atcatccagg
     7261 cctcggtttc ctgctgg
//
LOCUS       DASYCS010000011         4542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4060717,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000011 DASYCS010000000
VERSION     DASYCS010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4542
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4060717"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1262)
                     /locus_tag="VGM46_00455"
     CDS             complement(<1..1262)
                     /locus_tag="VGM46_00455"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006201260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HEY4191092.1"
                     /translation="MTTLAADREIESLLALHPKGFDLSLDRITRLLDRLGNPHDRLPP
                     VIHIAGTNGKGSCAAFARALLEAAGYRVHVHTSPHLVHWHERYRLAADGGGRFVEDKV
                     FAEAISRVAKANGGEAITVFEILTAVTFVLFSEHPADAAIIEVGLGGRFDATNVIKRP
                     AASVIMPVSLDHEAYLGDRVELIAAEKAGIIKRGRPVVVGAQESETAQAVLVETAERL
                     GCPTVVYGQDFLAYGENGRLVYQDDDGLMDLPPPRLPGRHQFANAAAAIAAVKAAGFD
                     IGERAAEQAMANVAWPGRMQKLTQGRLVDLAPRGAEIWLDGGHNPGAGAVVAEAMAEQ
                     EEKSPRPLFLISGMINTKDQSGYFSAFKGLVRHVYTVPGSFSDAGVPNDELAVRATEA
                     GLSAEPVSSVASALMLLRDTWDEAEPPPR"
     gene            complement(1324..2247)
                     /gene="accD"
                     /locus_tag="VGM46_00460"
     CDS             complement(1324..2247)
                     /gene="accD"
                     /locus_tag="VGM46_00460"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015314369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEY4191093.1"
                     /translation="MNWITNYVRPKINSMLGRRDIPENLWIKDPESGEMVFHKDLEEN
                     QYVIPSSGHHMKISAKERLKFFFDDGKFEMLENPKVALDPLKFRDEKRYVDRLKDAKS
                     KTGLEDAIVNALGSIEGLPVVATVQDFAFMGGSLGMAAGDAIVTAFETARQRKRPLIL
                     FAASGGARMQEGILSLMQLPRTTVGVDRLKEAGLPYIVVLTNPTTGGVTASYAMLGDV
                     HIAEPGALIGFAGPRVIEQTIREKLPDGFQRSEYLMEHGMVDMVVSRLELKTTIARLL
                     KMLLKVPETPKVAEPEILPPAVASAEARPQA"
     gene            complement(2290..3135)
                     /gene="trpA"
                     /locus_tag="VGM46_00465"
     CDS             complement(2290..3135)
                     /gene="trpA"
                     /locus_tag="VGM46_00465"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEY4191094.1"
                     /translation="MATNTRIDRRMAKLKQEGRPALVTYFMGGDPDYDTSLSIMKAMA
                     ASGADVIELGMPFSDPMADGPAIQAAGLRALKGGQTLAKTLKMAADFRKGDNETPIVL
                     MGYYNPIYVYGVNRFLADATASGIDGLIVVDLPPEMDEELCIPAVKAGINFIRLATPT
                     TDDKRLPKVLQNTSGFVYYVSMTGITGSALADTGKVAAAVQRIKGHTDLPVCVGFGVK
                     TAEQARVIGASADGVVVGTAIINAVANVLGPNGERTADPAEAVATLVTGLSQGVRAAR
                     LAAAE"
     gene            complement(3137..4357)
                     /gene="trpB"
                     /locus_tag="VGM46_00470"
     CDS             complement(3137..4357)
                     /gene="trpB"
                     /locus_tag="VGM46_00470"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004692070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEY4191095.1"
                     /translation="MNQPTMPNSFRTGPDNEGMFGIYGGRFVAETLMPLILELEKNWN
                     EVKEDPAFRAELQDLSTHYAGRPSKLYFAEGLTKHLGGAKIYFKREDLNHTGSHKINN
                     CLGQILLAKRMGKKRIIAETGAGQHGVASATVAARFGFPCVVYMGATDVARQSPNVFR
                     MKLLGAEVRPVSAGHGTLKDAMNEALRDWVTNVEDTYYLIGTAAGPHPYPELVRDFQS
                     VIGTEARAQILEQEGRLPDTIIAAVGGGSNAIGLYHAFLDDKDVRIIGVEAGGRGLDG
                     IEHCASMNAGRPGVLHGNRTYLLQNEDGQILDGHSISAGLDYPGVGPEHSWLKDTGRV
                     EYVPILDEEALEAFQLTTRVEGIIPALESAHAIAHAMKIAPAMGKDQVMIVNLSGRGD
                     KDVHTVAAMMGMEI"
     gene            complement(4385..>4542)
                     /locus_tag="VGM46_00475"
     CDS             complement(4385..>4542)
                     /locus_tag="VGM46_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006201256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-(5'-phosphoribosyl)anthranilate isomerase"
                     /protein_id="HEY4191096.1"
                     /translation="NAANISEALSLANPRGIDISSGVESAPGIKEPALIEKFFRAVST
                     ATGSRAA"
BASE COUNT          765 a         1426 c         1507 g          844 t
ORIGIN      
        1 cgcggcggcg gctcggcttc gtcccaggtg tcgcgcagca gcatcagggc gctggcgacg
       61 gaacttaccg gctcggcgga cagcccggcc tcggtcgccc gcaccgccaa ttcatcattg
      121 ggcacaccgg catcgctgaa actgcccggc accgtgtaga cgtggcgcac cagccccttg
      181 aaggcgctga aatagccgct ctggtccttg gtgttgatca tgccggagat gaggaatagc
      241 ggccttgggc ttttttcctc ctgctcggcc atcgcctccg cgacaacggc gccggcgccg
      301 ggattatggc cgccgtcgag ccagatttcg gctcccctgg gcgccagatc gaccagccgc
      361 ccctgcgtga gcttctgcat gcgccccggc caggcgacat tggccatcgc ctgctcggcg
      421 gcgcgctcgc cgatgtcgaa gccggcagcc ttgacggcgg cgatggcggc ggcggcattg
      481 gcgaactgat ggcggccggg caggcgcggc ggcggcaaat ccatcaagcc gtcatcgtcc
      541 tgatagacca ggcgaccgtt ttcgccatag gcaaggaaat cctgaccata gaccacggtg
      601 gggcagccaa ggcgctcggc ggtttcgacc agaacggctt gcgccgtctc gctttcctgc
      661 gcacccacca ccacggggcg gccacgcttg atgatgcctg ccttttcggc agcgataagc
      721 tcgacgcggt cgcccagata ggcttcgtga tcgagcgata ccggcatgat gaccgaggcg
      781 gcggggcgct tgatgacgtt ggtggcgtcg aagcggccgc caagtccgac ctcgatgatg
      841 gccgcatccg ccggatgctc ggaaaacagc acgaaggtca ccgccgtcag gatttcgaag
      901 acggtaatgg cttcgccgcc attggccttg gcgacgcgcg aaatggcctc tgcgaaaacc
      961 ttgtcctcga cgaacctgcc accgccatcc gccgccaggc gatagcgctc gtgccaatgg
     1021 accaggtgcg gggaggtgtg cacatgcacg cggtagccgg cagcctccag caaggcgcgc
     1081 gcgaaggccg cgcaggagcc tttgccgttg gtgccggcaa tgtggatcac cggcggcagt
     1141 ctgtcgtgcg ggttgcccag ccggtcgaga agccgcgtaa tgcggtcgag cgacaggtcg
     1201 aagcctttgg gatgcaacgc caacaggctt tcgatttcgc ggtcggcggc aagcgttgtc
     1261 atgatgcagt cctatgcgcg cgccaaatcg cgcgccatgc ggtttgcagt tggggccgtg
     1321 gtgtcaggcc tgcgggcggg cttcggccga ggcaacggca ggcggcagga tttccggctc
     1381 cgccaccttc ggcgtttccg gcaccttgag cagcatcttc aatagccgcg cgatggtcgt
     1441 tttcaactcc agccgggaga ccaccatatc gaccatgccg tgctccatca gatattcgga
     1501 gcgctggaag ccatcgggca gtttttcgcg gatcgtctgc tcgatgacgc gcggcccggc
     1561 aaagccgatc agcgcacccg gctcggcgat atgcacgtcg cccagcatgg cgtaggaggc
     1621 ggtgacgccg ccggtcgtcg gattggtcag caccacgata tagggcaggc cggcttcctt
     1681 cagccggtcg acaccgacgg tggtgcgcgg cagctgcatc agcgacagga tgccttcctg
     1741 catgcgcgcg ccgccggaag cggcaaacag gatcagcggc cgcttgcgct ggcgggccgt
     1801 ctcaaaggcg gtgacgatgg catcgccggc ggccatgccc agcgaaccgc ccatgaaggc
     1861 gaagtcctgc acggtggcga ccaccggcag tccctcgatg gaacccaagg cattgacgat
     1921 agcgtcttca aggccggtct tggacttggc gtccttcagc cggtcgacat agcgcttctc
     1981 gtcgcggaac ttcagcgggt cgagcgcgac cttggggttt tccagcattt cgaacttgcc
     2041 atcgtcgaag aagaatttca gccgctcctt ggccgaaatc ttcatgtggt ggccggaaga
     2101 aggaatgacg tactggttct cttccaggtc cttgtggaag accatttccc cgctctcggg
     2161 atccttgatc cagaggtttt cgggaatgtc gcggcggccc agcatcgaat tgatcttcgg
     2221 gcggacgtag ttggtaatcc agttcatcgc atcaggctcc tgtccctact tccgcgagag
     2281 gtaggaaaac tattcggcgg cagcaaggcg ggccgcgcgc acgccctgcg acaggccggt
     2341 gaccagcgtg gcgacggcct cggccggatc ggcggtgcgc tcgccattcg gcccgagcac
     2401 attggccacc gcattgatga tcgcggtgcc gacgaccacg ccatcggcgg aagcgccgat
     2461 gacgcgcgcc tgctcggcgg tcttgacgcc gaagccgacg cagaccggaa ggtcggtgtg
     2521 gcccttgatg cgctggacag cggcagccac cttgccggtg tcggccaggg ccgagccggt
     2581 gatgccggtc atcgacacgt aatagacgaa accggaggtg ttttgcagca ccttgggcag
     2641 gcgcttgtca tcggtcgtcg gggtggcgag gcggatgaaa ttgatgcctg ccttgacggc
     2701 tggaatgcaa agttcctcgt ccatttccgg cggcaggtcg accacgatca gcccgtctat
     2761 gccggaagcc gtcgcgtcgg ccaggaagcg attgacgccg tagacataga tcgggttgta
     2821 gtagcccatc agcacgatgg gcgtctcgtt gtcgcccttg cggaaatcgg cggccatctt
     2881 cagcgtcttt gccagtgtct ggccgccttt gagcgcgcgc aggccggccg cctggatcgc
     2941 cgggccatcg gccatcggat cggagaacgg catgccaagt tcgatgacgt cggcgcccga
     3001 agcggccatg gccttcatga tcgacagcga ggtgtcgtaa tccgggtcgc cgcccatgaa
     3061 ataggtgacg agggccgggc ggccctcctg cttcagcttg gccatgcggc ggtcgatgcg
     3121 ggtattggtg gccatgtcag atctccatgc ccatcatcgc ggcgaccgta tgcacgtcct
     3181 tgtcgccacg gcccgacagg ttgacgatca tgacctggtc cttgcccatg gccggcgcga
     3241 tcttcatcgc atgggcgatg gcgtgcgccg attccagcgc cgggatgatg ccttcgacgc
     3301 gggtggtcag ctggaaggcc tccagcgcct cttcgtcgag gatcggcaca tattcgacgc
     3361 ggccggtatc cttcagccag ctgtgctcgg ggccgacgcc gggataatcg aggcccgccg
     3421 agatcgagtg gccgtccaat atctggccat cctcgttctg caacaggtag gtgcggttgc
     3481 catgcagcac gcctggcctg ccggcgttca tcgaggcgca atgctcgatg ccgtcgaggc
     3541 cgcgcccgcc ggcttcgacg ccgatgatcc gcacgtcctt gtcgtcgagg aaggcgtggt
     3601 agaggccaat ggcgttggag ccgccgccga ccgccgcgat gatggtgtca ggcaagcggc
     3661 cttcctgttc cagtatctgc gcgcgcgctt ccgtgccgat caccgactgg aagtcgcgca
     3721 ccagttccgg ataggggtgc ggtccggcgg cggtgccgat gaggtaatag gtatcctcga
     3781 cattggtgac ccagtcgcgc agggcctcgt tcatggcgtc cttcagcgtg ccgtggccgg
     3841 cgctgaccgg gcgcacttcc gcgcccagca gcttcatgcg gaacacgttg gggctttggc
     3901 gggcaacatc ggtcgcgccc atatagacca cgcagggaaa gccgaagcgg gcggcaaccg
     3961 tcgccgaggc gacgccgtgc tggccggcgc cggtttcggc gatgatgcgc ttcttgccca
     4021 tgcgcttggc cagcaggatc tggccgaggc aattgttgat cttgtgcgaa ccggtgtggt
     4081 tcagatcctc gcgcttgaaa taaatcttgg cgccgcccag atgcttcgtc aggccctcgg
     4141 cgaaatagag cttcgaaggc cgcccggcat aatgggtcga caaatcctgc aattcggcgc
     4201 ggaaggcggg gtcttccttg acctcgttcc agttcttttc cagttcgagg atcagcggca
     4261 tcagcgtttc ggcgacgaaa cggccaccgt aaatgccgaa catgccctca ttgtcgggac
     4321 cggtgcggaa cgaattgggc atcgtcggct ggttcatcgc cgatctcctt gtaagactga
     4381 aacgtcaggc ggcgcggctg cctgtcgccg tggaaacggc ccggaagaat ttctcgatca
     4441 acgctggttc ctttatgccc ggcgcgctct cgacacccga cgagatgtcg atgccgcgcg
     4501 ggttggcgag gctgagagcc tcactgatat tggcagcatt ga
//
LOCUS       DASYCS010000012        14269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_5075200,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000012 DASYCS010000000
VERSION     DASYCS010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 14269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14269
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5075200"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1722)
                     /gene="ligA"
                     /locus_tag="VGM46_00480"
     CDS             complement(<1..1722)
                     /gene="ligA"
                     /locus_tag="VGM46_00480"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018428579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEY4191097.1"
                     /translation="MSEQAVDALTEGEAATELERLAKEIAGHDLRYHAEDAPTISDAD
                     YDALRRRNLAIEQRFPDLVREDSPSRTVGAAVSEKFGKVVHVVPMLSLDNAFADQDVA
                     DFVGRIRRFLRLGADDPVAISAEPKIDGLSLSLRYEAGRLVTAATRGDGQVGENVTAN
                     ARTVADIPNVLSGDFPDVLEVRGECYMGHADFAELNRRNGEAGKQTFANPRNAAAGSL
                     RQLDTAITASRPLRFFAYAWGEVSAMPADTQMGMVGAFERFGFKVNRLMKLFDSVEGL
                     LEQYRLIESNRATLGYDIDGVVYKVNSLALQERLGFVSRSPRWAIAHKFPAEKATTIL
                     TGIDIQVGRTGALTPVARLAPVTVGGVVVTNATLHNEDYIKGIGNNGLPIREGRDIRV
                     GDTVRIQRAGDVIPQIIDIVPEKRPANSVPYVFPDHCPACNSRAVREEGEAVRRCTGG
                     LICPAQAVERLRHFVSRGAFDIEGLGEKQIEFFFHASDPALKIQSPADIFTLKARQAV
                     SLTKLENIDGFGATSVGKLFAAIDDRRRIGFSRFLYALGIRHIGDTNAKRLARHFLSF
                     QALREAGE"
     gene            complement(1853..3529)
                     /gene="recN"
                     /locus_tag="VGM46_00485"
     CDS             complement(1853..3529)
                     /gene="recN"
                     /locus_tag="VGM46_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018428580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEY4191098.1"
                     /translation="MLARLSIRDIVLIEKLDIDFSAGLSVLTGETGAGKSILLDALSL
                     ALGARGDASLVRHGSAQGQVIAVFDVPRNHPARALLADNAIEDEGDIILRRLQTADGR
                     TRVFVNDQPSSVTLMRDVGRALVEIHGQHDERALVDPGAHRDVLDTFGGHAGAARSTA
                     EAWRLWRSSEQEFSRARASVEAAAREADYLRASVAELVKLDPQPGEETDLAELRAAMM
                     RAEKIASEIQDAQDVLSGPSSPLPELASLLRRLQRKVTDAPGLLDDVVKSLDEAMLSL
                     DAAQSGVEAALRAAEYDPQRLEKAEERLFSLRAASRKYAVAVDDLAQLRDTMSADLAD
                     LDAGEGRLHEMEKQAAATRNAYDVSAAQLSELRHAAAVGLTRAVMAELPALKLERAEF
                     IVAMTSEAESRMEEGIDSVEFWVRTNPGTRPGPMMKVASGGELSRFLLALKVALADRG
                     SAPTLVFDEIDTGVGGAVADAIGQRLARLSLRVQVLSVTHAPQVAARAATHFLISKSG
                     SADRVATGIAEMDRAARQEEIARMLAGATITEEARAAADRLLRENTAAAS"
     gene            complement(3537..4406)
                     /locus_tag="VGM46_00490"
     CDS             complement(3537..4406)
                     /locus_tag="VGM46_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019859082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEY4191099.1"
                     /translation="MFFQQTGRSQASARSVLLALSVAVAPLLLSACMSSEKDIDLSTY
                     VDQTEPADVLYNQGLANLNAGRLSEASKKFDAVDKQHPYSEFARKSMVMGAFADYRQG
                     NYEEAIGSAKRYLTLYPTTPDAAYAQYIIGLSYYRQIKDVTQDQKETRQTLQIMQDLV
                     TRWPDSEYVEDAKAKIRFANDQLAGKEMQIGRYYLERREYIAAIKRFRTVVENYSNTR
                     HVEEALSRLVESYFAMGLTDEAQTAAAVLGHNYPDSPWYKDSYKLLQGGGLAPRENAG
                     SWISKAGKLITGA"
     gene            complement(4621..5571)
                     /gene="lpxC"
                     /locus_tag="VGM46_00495"
     CDS             complement(4621..5571)
                     /gene="lpxC"
                     /locus_tag="VGM46_00495"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013894677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEY4191100.1"
                     /translation="MGFDLHDYQTTIKSRVTLTGIGVHSGREVSAHFSPADADTGIVF
                     HATDESGAGREFHALVSEVGATDLCTMLGDPSGQHIATVEHLMACLFGLGIDNLAIEI
                     EGGEVPILDGSSIMFVEAFDQAGIEFLSVKRRYIRVMKPVRIENGASWAEFRPYAGTR
                     FEVEIDFESPAIGRQSFASDMNAEIFRRDIARSRTFGFMKDVERLWAAGYALGSSLEN
                     SLVIGHDNRVINLGGLRYPNEFARHKTLDAMGDLALAGARFIGCFRSYRGGHRLNATA
                     LRRLLSDRSAFEIVETTKRDRGHMAEMNAVAAPAYAPWML"
     gene            complement(5844..7523)
                     /gene="ftsZ"
                     /locus_tag="VGM46_00500"
     CDS             complement(5844..7523)
                     /gene="ftsZ"
                     /locus_tag="VGM46_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEY4191101.1"
                     /translation="MTINLQKPDITELKPRITVFGVGGGGGNAVNNMITAGLRGVEFV
                     VANTDAQALTMSKADRLIQLGAHVTEGLGAGSQPEVGRAAAEECIDEIIDHLSNTHMC
                     FVTAGMGGGTGTGAAPVVARAAREKGILTVGVVTKPFHFEGQRRMKTADLGIEELQKC
                     VDTLIVIPNQNLFRLANDKTTFADAFAMADQVLYSGVACITDLMVKEGLINLDFADVR
                     SVMREMGKAMMGTGEASGEGRAMAAAEAAIANPLLDETSMKGAKGLLISITGGRDLTL
                     FEVDEAATRIREEVDQDANIILGATFDEELEGVIRVSVVATGIDKSAEQIASAPIAIR
                     MPAQAQKPATVRPVAPAAEIRPVAAPVHQQVQEPRAIDPVAEAIQLAEANAAEMAQAR
                     PVAAAYEDDFRPVNKLFQAPPAQPQVQFAAAQAIQQAAPREMPQPVAAAPQRMPRVED
                     FPPQVKAEVEAKAHPLDQEDRGPMGLLKRLTNGLTRREEEPARLQPAQPREPRLRQAA
                     PETRRLASQDAQLYAPRRGQLDNHGRLEPQTRAAHDDDQLEIPAFLRRQAN"
     gene            complement(7588..8892)
                     /gene="ftsA"
                     /locus_tag="VGM46_00505"
     CDS             complement(7588..8892)
                     /gene="ftsA"
                     /locus_tag="VGM46_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015317215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEY4191102.1"
                     /translation="MSWLGGHGDPAHRSGIVTVLDVGSSKVCCVVAKLKPCEDGKLLR
                     GRSHRIQVMGIGHQKSEGVKSGVVVDLDRAEHAVRLAVDAAERMAGLTVDSLIVNMTA
                     GRLKSESFAATINLGGHQAGEGDVKRVLAAGAKQALKTEREVVHSLPVGFSLDAERGV
                     RDPRGMVGDTLGVDMHVLTGDSAPMRNLELCINRSHLSVERMVATPYASGLAALVDDE
                     LEMGAACIDMGGGTTTISVFNEGKFIHADALPIGGNHVTLDLAKGLSTSIAAAERLKV
                     MHGSALPGSADDRDLVSIQPIGEEGDIAAQVPRSMMTRIIRARIDETLELLRDRINKS
                     GYGNVVGRRVVLTGGASQLAGLPEAARRILGRNVRIGRPLGVAGLPEAAKGPAFSVPV
                     GLLIYPQMAAFESHPAQGMSRFKMTGTGGRLHRMSEWLRDSF"
     gene            complement(8889..9830)
                     /locus_tag="VGM46_00510"
     CDS             complement(8889..9830)
                     /locus_tag="VGM46_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019859085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEY4191103.1"
                     /translation="MSAVRPGQGYGRGSAAPALFGISLSLDHFVLPRWLRKPVRMFTH
                     LGRGNFTPPRFAASLMTAALLASSSAYGAYVGGHADSIVQGFTARTGFAVDQIKVVGN
                     HETSEIDILGSLGLDGWTSLIGFDVDAARDRIAMLPWVETAAVRKIYPHTLEVHVEER
                     EAFALWQKGGELSVIERSGRVIAPYSGGKQALLPLFVSADAPVLAPDLLAKIDKFPEL
                     AARVRGYVRVAGRRWDLRLRNGVTVKLPEMGEDEAIASLAALDREDGLLGRDIVAVDM
                     RLPDRLVVQLSPEAESARQAALLDKAKSPARKKEANI"
     gene            complement(9818..10744)
                     /locus_tag="VGM46_00515"
     CDS             complement(9818..10744)
                     /locus_tag="VGM46_00515"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018494805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEY4191104.1"
                     /translation="MKNKHVAVLMGGFSSERPVSLSSGAACAATLEEEGYQVTRVDVG
                     RDVGSVLEALKPDVCFNALHGPFGEDGTIQGILEYLAIPYTHSGVLASALAMDKEQAK
                     KIAKAAGMPVAESKVTSRFTIRDKHPMKPPYVVKPVNEGSSFGVVIVQEGQSHPPQII
                     SSPEWGFGERVMIERYVHGRELTCAVMGDVALGVCEIIPTGHSFYDYDSKYVAGGSKH
                     ECPAKISPNIYQKIQTLALKAHQAIGCRGVSRSDFRYDDRHSENGEVVWLEVNTQPGM
                     TPTSLVPEIAAQAGHSFGDLLSWMVEDASCLR"
     gene            complement(10998..11963)
                     /gene="murB"
                     /locus_tag="VGM46_00520"
     CDS             complement(10998..11963)
                     /gene="murB"
                     /locus_tag="VGM46_00520"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013894672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEY4191105.1"
                     /translation="MTRGEQLLASLGDQLAGLRGRITPNAEMDKITWFRAGGLADALF
                     QPADEDDLAAFLKAVPQEVPLTMVGIGSNLLVRDGGIPGFVVRLSAKGFGEAEVISPT
                     RIRAGSAIPDKRVAAVAYEAGIGGFHFYHGIPGAIGGALRMNAGANGVETRERVVEVR
                     ALDRKGNFHTLSNADMGYAYRHSAAPADFIFTSAVFEGFAEDKAAIKAAMDAVQHHRE
                     TVQPIREKTGGSTFKNPEGHSAWKVIDEAGCRGLMVGGAQMSPMHCNFMINTGSATGY
                     ELEYLGETVRARVLEHSGIRLHWEIKRIGNFRLGHAVQEFLGQLL"
     gene            complement(11960..13357)
                     /gene="murC"
                     /locus_tag="VGM46_00525"
     CDS             complement(11960..13357)
                     /gene="murC"
                     /locus_tag="VGM46_00525"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEY4191106.1"
                     /translation="MKMPQTIGLVHFIGIGGIGMSGIAEVLHNLGYKVQGSDQADGAN
                     VQRLRDKGIECFVGHAAENLGDAEVVVVSTAIKKSNPELKAARERLLPIVRRAEMLAE
                     LMRFRQAVAIGGTHGKTTTTSMVATLLEAGGLDPTVINGGIINAYGTNARMGDGEWMV
                     VEADESDGTFLKLPADIAVVTNIDPEHLDHYGSFDAVREAFRQFVENVPFYGFGVMCT
                     DHPEVQALVSRIEDRRVITYGANAQADVRFTNHRMNGASSEFDVVIRNRKGRQTEIGG
                     LRLPMPGRHNVSNATAAIAVAHELGLSPEAIKKGLSSFGGVKRRFTHTGSWNGVDVFD
                     DYGHHPVEITAVLRAARESTKGRVIAIAQPHRFTRLHDLFDDFASCFNDADTVMIAPV
                     YTAGEDPIEGATSDALVARLRAGGHRDARYIEGPEAIAPIVRGLAGPGDFVVFLGAGS
                     ITQWAYALPRELNGS"
     gene            complement(13354..>14269)
                     /locus_tag="VGM46_00530"
     CDS             complement(13354..>14269)
                     /locus_tag="VGM46_00530"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEY4191107.1"
                     /translation="LRSLWTLWRGFRQSSTLIGKLKPKVVIGFGGYPTLPPLFAATRR
                     RVPTLVHEQNAVMGRANKALAPRVTAIAGGFLKAAGPYARKIAETGNPVRQAVLKVAD
                     TPYERPQADGPVRLLIFGGSQGARYFSDTVPEAIAVLSATLRQQLRIVQQARSEDEAR
                     VKARYGELGVEAEVAPFFADLPARMAAAQLVVARSGASTVLELSVIGRPAILVPLPHA
                     LDDDQGHNAQILVDAGGAVMRRQGDLGVEALAAELGSLLADGDRLASMASGARSVGRR
                     DATLLLADLTEAIASGKSAADFKKEMRP"
BASE COUNT         2614 a         4737 c         4337 g         2581 t
ORIGIN      
        1 ctcacccgct tcgcgcaaag cctggaagga caggaaatgg cgcgccagcc gcttggcgtt
       61 ggtatcgcct atatggcgga tgccaagggc atagaggaag cgtgaaaacc cgatccgacg
      121 cctgtcgtcg atggcggcaa acagcttgcc gaccgatgtc gcgccgaacc cgtcaatatt
      181 ctcaagcttg gtaagcgaca cggcctgccg cgccttcagt gtgaaaatgt cggcgggcga
      241 ctggattttc agcgccgggt cactggcgtg gaagaaaaac tcgatctgct tttcgcccag
      301 cccctctatg tcgaaagcgc cgcgcgaaac gaaatgacgc agccgttcca ccgcctgcgc
      361 cgggcagatc agcccgccgg tgcagcgccg caccgcctcg ccctcctcgc gcacggcccg
      421 gctgttgcaa gccgggcaat ggtctggaaa gacgtaaggg acgctatttg ccggccgctt
      481 ttccggcaca atatcgatga tctgcggaat gacgtcgccc gcgcgctgta tgcgcacggt
      541 gtctccgacg cgaatgtcgc gcccttcgcg gatcggcagg ccgttgttgc cgatgccctt
      601 gatgtaatct tcattgtgca gggtggcgtt ggtcaccacc acgccgccca ccgtcaccgg
      661 cgccagccgc gccaccggcg tcagcgcacc cgtgcggcca acctgaatgt cgatgccggt
      721 aaggatcgtc gtcgcttttt cggcggggaa tttatgcgca atcgcccaac gcggcgagcg
      781 tgagacgaac cccagccgct cctgtagcgc aaggctgttc accttgtaga cgacgccgtc
      841 aatgtcgtag ccaagcgtcg cgcggttgga ttcgatcagc cggtattgct caagcagacc
      901 ttcgacgctg tcgaacagct tcatcagccg gtttaccttg aaaccaaacc gctcgaatgc
      961 ccccaccatg cccatttgcg tatcggccgg catcgcgctg acctcgcccc acgcataggc
     1021 gaagaagcga agcggccggc tggccgtgat ggcggtatcg agctggcgca gcgatcccgc
     1081 cgccgcgttg cggggattgg cgaaggtctg cttgccggcc tcgccgttgc ggcggttcag
     1141 ctcggcaaaa tcggcatgcc ccatatagca ttcgccgcgc acctccagca cgtcggggaa
     1201 atcacccgaa agcacgttgg gaatgtcagc gaccgtgcgc gcattggcgg tgacgttttc
     1261 gcccacctgc ccgtcgccgc gcgttgccgc cgtcaccagc cgcccggctt catagcgcag
     1321 cgacagcgac aggccatcga ttttcggctc ggcgctaatg gcgacgggat cgtccgcacc
     1381 aagccgcagg aaccgccgga tgcgcccgac gaaatcggcc acgtcctgat cggcgaaggc
     1441 attgtcgagc gacagcatcg gcacgacatg aacgaccttg ccgaatttct ccgaaaccgc
     1501 cgcgcctacc gtgcgcgacg gcgaatcctc gcgtaccagg tcgggaaagc gctgctcgat
     1561 ggcaaggttg cgccgccgca gggcatcata gtcggcgtcc gagatcgtcg gcgcatcctc
     1621 ggcatgatag cgcaggtcgt ggcccgcgat ctcctttgcc agccgctcca actcagtggc
     1681 ggcctcgcct tcagtcagcg catcgacggc ttgttcggac atggcttcta tccatggtgt
     1741 caaagacatg accataaatc agatttgaag cggggaatat cgtgaaaatc gtacacgaac
     1801 agcgggatgc tgccatatcc tgaatcaatc catcatcgcc aaaacggtcg cgtcaggaag
     1861 cggcggcggt attttcccgc aaaagccggt ctgcggcggc gcgcgcttcc tcggtgatgg
     1921 tcgcgccggc cagcatacgc gcgatttctt cctgcctggc cgcgcggtcc atctcggcga
     1981 tgccggtcgc cactctgtcg gcgctgcctg atttggaaat caggaaatgg gtcgccgccc
     2041 ttgccgccac ttgcggcgcg tgcgttaccg acagcacctg cacgcgcagc gatagccgcg
     2101 ccagccgctg gccgatggca tccgccaccg ccccgccgac accggtgtcg atctcgtcga
     2161 acacaagcgt tggcgccgag ccgcgatcgg ccaacgccac cttcaacgcc aaaaggaagc
     2221 gcgagagctc gccgcccgac gctaccttca tcatcggtcc gggccgcgtg ccggggttgg
     2281 tgcgcaccca gaactcgacg ctgtcgatgc cttcttccat gcggctttcc gcttcgctgg
     2341 tcatggcgac gatgaactcg gcgcgttcca gcttcaatgc cggcagttcg gccatcaccg
     2401 ccctggtcag accgaccgcg gcggcgtggc gaagctcgga aagctgtgcg gccgaaacgt
     2461 cgtaggcgtt gcgcgtggcg gccgcctgtt tctccatttc gtgcaatctg ccttcgccgg
     2521 catcgagatc ggcgaggtcg gccgacatgg tgtcgcgcaa ttgcgccaga tcatcgaccg
     2581 ccaccgcata tttgcgcgaa gccgcgcgca gcgaaaacag ccgctcctcc gccttctcca
     2641 gccgctgcgg gtcgtattcg gcagcgcgca gcgccgcttc gacaccggat tgggcggcgt
     2701 ccagcgacag catcgcctcg tccagcgact tgaccacatc gtccagcagg cccggcgcgt
     2761 ccgtgacctt gcgttgcagc cggcgcaaaa ggcttgccag ttcgggcaag ggcgaggacg
     2821 gaccggaaag cacatcttgc gcgtcctgga tttccgaggc gatcttttcg gcgcgcatca
     2881 tcgcggcgcg caattcggcc agatcggtct cttcgccggg ctgtggatcg agcttgacca
     2941 actccgccac cgacgcgcgc agataatcgg cctcgcgcgc cgctgcctca acactggcgc
     3001 gcgcgcgcga aaactcctgt tcgctggagc gccacaaccg ccacgcctcc gctgtcgagc
     3061 gcgcggcccc ggcatggccg ccaaacgtgt cgagcacgtc gcgatgcgcg ccgggatcga
     3121 ccagcgcgcg ctcgtcgtgc tggccatgga tttccaccag cgcccgcccg acatcgcgca
     3181 tcagcgtcac gctggaaggc tggtcattga cgaaaacgcg ggtacgtccg tccgccgtct
     3241 gcaagcggcg caggatgatg tcaccctcgt cctcgatggc gttgtcggca agcagcgcgc
     3301 gggcgggatg gtttcgcggc acgtcgaaca ccgcgatgac ctggccctgc gccgacccat
     3361 ggcgcaccag cgatgcgtcg ccgcgcgcgc ccagcgccag cgacagtgca tcaagcagga
     3421 tggatttgcc ggcgcccgtc tcgcctgtca gcacagagag gccggccgaa aaatcgatgt
     3481 ccagcttttc gatcaggacg atatcgcgaa tcgaaagcct ggcgagcatt acagcatcag
     3541 gcgccggtaa tcagtttccc ggccttggaa atccacgaac cggcattttc gcgcggcgca
     3601 agcccgccgc cttgcaacag cttgtaggaa tccttgtacc atgggctgtc cggatagttg
     3661 tggccgagca cggcggcggc cgtctgcgcc tcgtcggtca ggcccattgc gaaataggac
     3721 tcgaccagac gcgaaagcgc ctcctcgacg tggcgggtat tggaataatt ttcgacaacg
     3781 gtgcggaatc gcttgatcgc cgcgatatac tcgcgccgtt ccagataata gcggccgatc
     3841 tgcatctcct tgcctgccag ctggtcgttg gcgaagcgaa tcttggcctt ggcgtcctcg
     3901 acatattccg aatcgggcca gcgcgtgacc agatcctgca tgatctggag ggtctggcgg
     3961 gtctctttct gatcctgcgt aacgtccttg atctgccggt aatagctcag gccgatgata
     4021 tactgggcat aggccgcatc cggcgtcgtc ggatagagcg tcaggtaacg cttggccgag
     4081 ccgatggctt cttcgtaatt gccttggcga taatcggcga acgcgcccat caccatcgat
     4141 ttgcgcgcaa attccgaata gggatgctgc ttgtccaccg catcgaattt cttgcttgcc
     4201 tcggacagac gaccggcgtt caggttggcc agcccctgat tgtagagcac gtcggccggc
     4261 tcggtctggt cgacataggt cgaaaggtcg atgtcctttt cggacgacat gcaggccgac
     4321 agcagaagcg gcgcaacggc caccgaaagc gccagcagaa ccgacctcgc cgaagcctgc
     4381 gatcgacccg tttgttggaa aaacatgatc cggttttgcc ctttcggcgt cattcttctc
     4441 atcggcacgc agccatagcc cataatcgcc gtgcgcggca acgctatcgt ccggcaatcg
     4501 tgcatttttg tgtccgcacg gaaaagctgc ctcaacacag cgatttcgga cgcttgtgat
     4561 gcattcgcgc aacaataaca ggctgaccgg cgccaaaagc cggccgatag gctacgggcc
     4621 tcaaagcatc cagggcgcgt aggccggagc cgcgacggcg ttcatttccg ccatatggcc
     4681 gcggtcgcgc ttcgtcgttt ccacgatctc gaaagccgac cggtccgaca gcaaccggcg
     4741 caacgccgtc gcattcagcc gatggccgcc ccgataggaa cgaaaacagc cgatgaaacg
     4801 cgcgccggcc aaggccaggt cgcccatggc atccagcgtc ttgtggcgcg cgaactcgtt
     4861 tggatagcgc aggccgccca gattgatgac gcggttgtcg tgcccgatca ccagcgaatt
     4921 ttccagcgag gagccgagcg catagccggc ggcccaaagc cgctcaacgt ccttcatgaa
     4981 gccaaaggtg cgcgaacgcg caatgtcgcg gcgaaaaatc tcggcattca tgtccgaggc
     5041 gaaggactgg cggccgatcg ccgggctttc gaagtcgatt tccacctcga accgcgttcc
     5101 ggcataggga cgaaattcag cccatgacgc gccattctcg atgcgcaccg gcttcatcac
     5161 gcggatatag cgccgcttga ccgacaggaa ctcgatgccg gcctggtcga aggcttcgac
     5221 gaacatgata gaactgccgt ccaggatcgg cacttcgccg ccctcgatct cgatggccag
     5281 attgtcgatg ccaagcccga acaggcaagc catcaaatgc tcgacggtgg cgatatgctg
     5341 gccggacgga tcgccgagca tggtgcagag atcggtcgcg ccgacttcgg aaaccagggc
     5401 atgaaactcg cgcccggctc cgctttcatc cgtggcgtgg aacacgatgc ccgtatcggc
     5461 gtcggccggc gaaaaatgcg cgctgacttc ccggccgcta tggacaccta tgccggtaag
     5521 cgtcacccgc gactttatcg tcgtttgata gtcgtgcaaa tcaaacccca tcacccaaac
     5581 cgtcatggcc cgccctaccg gtccaacagt cctggctcta tcttccgcag tcgcgcctct
     5641 taccccgcta ggcccgcctg ctgcaattca acagtcaaga taatgtcgag acaatgagtc
     5701 tccaaatcac ggtttctttc ccggtgttac gacgcacagg gcgtagaaac cgtgctaaca
     5761 gattgtttta cataccaatt aataaataat gggcaggcgg tttcccgcct gcccattaaa
     5821 gatcgttaca gtctgttaac agatcagttc gcttgccggc gaaggaaagc cgggatttcc
     5881 agctggtcgt cgtcatgggc ggcgcgcgtc tgcggctcaa ggcggccatg attgtccaac
     5941 tggccacgcc gaggcgcgta gagttgggcg tcctggctgg caaggcggcg cgtttccggt
     6001 gcggcctggc gcagccttgg ctcgcgcggc tgtgccggct gcaagcgggc aggctcttcc
     6061 tcgcggcggg taaggccgtt ggtcagccgc ttgagcaggc ccatcggccc acggtcttcc
     6121 tggtccagcg gatgcgcctt ggcttccacc tccgccttca cctgcggcgg gaaatcctcg
     6181 acgcggggca tgcgctgcgg cgcggcggca acgggctggg gcatttcgcg cggcgcggcc
     6241 tgctggatag cctgcgcggc ggcgaactgg acctgcggct gcgcaggcgg tgcctggaac
     6301 agcttgttga ccggacggaa gtcgtcctca taggcggcgg caaccggccg tgcctgcgcc
     6361 atctccgctg cattggcttc cgccagctgg atagcttcgg caaccgggtc gatcgctcgc
     6421 ggctcctgaa cctgttgatg gacaggcgct gcaaccgggc ggatttcggc agccggggcg
     6481 actgggcgaa ccgttgccgg cttctgcgcc tgggcaggca tgcggatggc gatcggcgcg
     6541 gaagcgatct gctcggccga cttgtcgatg ccggtggcaa cgaccgagac gcggatgacg
     6601 ccttccagtt cctcgtcgaa agtagcgcca agaatgatgt tggcgtcctg gtcgacttcc
     6661 tcgcgaatgc gggtagccgc ctcgtccact tcgaacaggg tgaggtcacg gccgccggtg
     6721 atggagatca gcaggccctt cgcacctttc atcgaggtct cgtcgagcag cggattggcg
     6781 atcgcggctt cggcggcggc catcgcgcgg ccctcgcccg atgcctcgcc ggtgcccatc
     6841 atcgccttgc ccatttcgcg catcaccgag cgcacatcgg cgaagtccag gttgatgagg
     6901 ccttccttga ccatcaggtc ggtaatgcag gcgacgcccg aatagagcac ctggtcggcc
     6961 atggcgaagg catcggcgaa agtggtcttg tcattggcca gccggaacaa attctggttg
     7021 gggatgacga tcagcgtatc gacgcatttt tgcagttcct cgatgccgag gtccgccgtc
     7081 ttcatccggc gctggccttc gaagtggaac ggcttggtga cgacgccgac ggtgaggatg
     7141 cctttttcgc gcgccgcgcg ggcaacgacg ggagcagccc cggtgccggt gccgccgccc
     7201 atgcccgccg tgacgaagca catatgggta ttggagaggt gatcgatgat ctcatcgatg
     7261 cattcttcgg ccgccgcgcg gccgacctct ggctgcgaac ccgcgcccag gccttccgtg
     7321 acatgcgcgc caagctggat cagcctgtcg gccttcgaca tggtcagcgc ctgcgcatcg
     7381 gtgttggcca cgacgaactc gaccccgcgc agtccagccg tgatcatgtt gttgacggca
     7441 ttgccgccgc cgccgccgac gccgaaaacg gtgatgcggg gcttgagctc cgtaatgtcg
     7501 ggcttttgca gattgatggt catatcctcg tcctttgcct ttcccgccgc ttcgccgtcg
     7561 cggccgcctt tggggctgcc cccggaatta aaaactgtct ctcaaccact cactcatacg
     7621 gtgcagtcgc ccgcccgtgc ccgtcatctt gaaacgggac attccttgcg cgggatggct
     7681 ctcgaaagca gccatctgcg gatagatcaa aagcccgacc ggcaccgaga acgccggccc
     7741 cttggccgcc tccggcaagc cggcgacgcc aagcgggcgg ccgatgcgga cattgcgtcc
     7801 cagaatgcgc ctcgccgctt ccggcaggcc ggcaagctgg ctggcgccac cggtaagcac
     7861 cacgcgccgg cccaccacat tgccgtagcc cgacttgttg atgcggtctc gcagcagttc
     7921 cagcgtctcg tcgatgcggg cgcgaatgat gcgcgtcatc atcgagcgcg gcacctgcgc
     7981 ggcgatatcg ccttcctcgc cgatcggctg gatcgaaacc agatcgcggt cgtcggcgct
     8041 gcccggcagg gccgagccat gcatgacctt cagccgttcc gccgccgcga tcgatgtcga
     8101 aaggcctttc gccagatcga gcgtgacatg attgcccccg atgggaagcg catcggcgtg
     8161 gatgaacttg ccttcgttga acacggaaat cgtcgtcgtg ccgccgccca tgtcgatgca
     8221 ggcagcgccc atttccaatt cgtcgtcgac cagagccgcc agcccgctgg catagggcgt
     8281 cgccaccatg cgctcgaccg aaagatgcga gcgattgatg caaagctcca gattgcgcat
     8341 tggcgccgaa tcgccggtca acacatgcat gtcgacgccg agcgtatcgc cgaccatgcc
     8401 gcgcgggtcg cgcacgccgc gctccgcatc gagcgaaaag cccaccggaa gcgaatgaac
     8461 cacctcgcgc tcggtcttca gcgcctgctt ggcgcctgcc gccagcacgc gcttgacgtc
     8521 gccttcgccg gcctggtgcc cgccaaggtt aatggtggcg gcaaagcttt cgctcttcaa
     8581 ccggccagcc gtcatgttga cgatcagcga atccaccgtc agcccggcca tgcgctccgc
     8641 cgcatcgacg gcaaggcgaa cggcatgctc ggcccggtcg aggtcgacga ccacgcccga
     8701 cttcacgcct tccgatttct ggtggccgat gcccatcacc tggatgcggt gcgaacggcc
     8761 acgcaaaagc ttgccgtcct cgcacggctt cagcttcgcc accacgcaac agaccttgct
     8821 ggaccctacg tccagcaccg tgacgatgcc ggaacggtgc gcggggtcgc catggccgcc
     8881 aagccagctc atatgttcgc ctctttcttg cgggcgggag atttcgcctt gtcgagcagc
     8941 gccgcctgcc gcgcgctctc cgcctcgggc gacagctgca cgaccagccg gtcgggcaac
     9001 cgcatgtcga cggcaacgat atcgcggccc agcaggccat cctcgcggtc gagcgcggcc
     9061 agcgaagcga tggcttcgtc ctcgcccatt tccggcagct tgacggtaac gccgttgcgc
     9121 agcctcaaat cccagcgccg gccggcaacc cggacgtagc ccctcacgcg cgcggccagt
     9181 tccggaaact tgtctatctt ggcaagcaga tcgggcgcaa ggactggcgc atcggcgctg
     9241 acgaacagcg gcaacagggc ctgcttgccg cccgaataag gcgcaatcac acgaccggac
     9301 cgttcgatga cggaaagttc gccgcccttt tgccaaagcg cgaaggcttc gcgctcctcg
     9361 acatgaactt ccagcgtatg cgggtaaatc ttgcggacgg ccgccgtttc gacccaggga
     9421 agcatcgcga tgcggtcgcg cgcggcatcc acatcgaagc cgatcagcga cgtccagcca
     9481 tcgaggccga ggctgcccag aatatcgatc tccgaagttt catgattgcc gaccaccttg
     9541 atctggtcga cggcaaaacc ggtgcgggcg gtaaagccct gcacgatgct gtcggcatgg
     9601 ccgccgacat aggcgccata ggcgctgctg gaggcaagaa gcgccgccgt catcagcgag
     9661 gcggcgaagc gaggcggcgt gaaattgccg cgccccagat gcgtgaacat gcgcaccggc
     9721 ttgcgcagcc atcgcggcag cacgaaatgg tcgagcgaca gggagatgcc gaacagcgcc
     9781 ggcgctgccg aaccccttcc gtatccttga cccggcctca ccgcagacac gacgcgtcct
     9841 ccaccatcca actcaacaaa tcaccaaacg aatgccctgc ctgggcagca atttccggca
     9901 ccagcgacgt cggcgtcatg cccggctgcg tgttgacttc cagccagacg acctcgccat
     9961 tttccgaatg acggtcgtcg tagcggaaat cagaccggga gacgccccga cagccgatcg
    10021 cttggtgagc cttgagcgcg agtgtttgta ttttttggta aatattcggt gaaatttttg
    10081 ccgggcattc gtgttttgag ccgccggcga catatttcga atcgtaatcg tagaaggaat
    10141 ggccggtcgg gatgatctcg cagacgccaa gcgccacatc gcccatcacc gcgcacgtca
    10201 attcgcgccc atgcacgtag cgctcgatca tcacgcgctc gccgaatccc cactcgggag
    10261 aggaaatgat ctgcggagga tgcgactgcc cctcctgcac gatcacgacg ccgaagctcg
    10321 atccttcatt caccggcttg acgacataag gcggcttcat cgggtgcttg tcgcgaatgg
    10381 tgaagcggct ggtcaccttg gattccgcca ccggcatccc ggcagccttg gcaattttct
    10441 ttgcctgttc cttgtccatg gccagcgccg acgccagcac gcccgaatgc gtatagggaa
    10501 tggccagata ttcgaggatg ccctggatgg ttccgtcttc cccgaaagga ccatgcagcg
    10561 cgttgaaaca aacatcaggc ttgagcgctt cgagcaccga gccgacatca cgcccgacat
    10621 cgacgcgcgt gacctggtac ccctcttcct caagcgtggc ggcgcaggcg gcgcccgagg
    10681 acaacgacac gggccgctcg gacgaaaatc cacccatcag gacggccaca tgcttgtttt
    10741 tcataccccg tcatcccctt tgcggcgggt tcaaatcaag cgtttcgcag aagtgagtcc
    10801 gagtctcccg gcaggttgcc ccccagacgg aaaacgcgct cacgcgtcga tgcgactcaa
    10861 atcaggaaac gcttcagggc agagaatcag agagttgatt cagtggcaag ggccaacgat
    10921 tccgcgagat tcactttcgc ggaatttcgt gacgaaaagt gatgcgttga gtctttgagg
    10981 ccacgcttgc cgagacgcta aagtagctgg ccaagaaact cctgcacggc atggcccagc
    11041 cggaaattgc cgatgcgctt gatttcccag tgcagcctga tgccggaatg ttccagcacc
    11101 cgcgcccgca ccgtctcgcc cagatactca agctcgtagc cggtggcaga gccggtattg
    11161 atcatgaagt tgcaatgcat cggcgacatc tgcgcaccgc cgaccatcag gccgcggcaa
    11221 ccggcttcgt cgatcacctt ccaggccgaa tggccttcgg ggttcttgaa ggtagagcca
    11281 ccggtctttt cgcggatcgg ctgcaccgtt tcgcggtgat gctggacggc atccatcgcc
    11341 gccttgatgg cggctttgtc ctcggcaaag ccctcgaaca ccgccgaggt gaagatgaaa
    11401 tcggcgggtg ctgccgaatg gcggtaggca taccccatgt cggcattgga aagcgtgtgg
    11461 aaattaccct tgcggtcgag cgcgcgcacc tcgacgacgc gctcgcgcgt ctcgacgccg
    11521 ttcgcgcccg cattcatccg aagcgctccg ccaatggcgc ccggaatgcc atgatagaaa
    11581 tgaaacccgc cgatgccggc ctcataggcg acagccgcga cgcgcttgtc ggggatggcg
    11641 gaccccgccc ggatgcgggt cggcgaaatc acttccgcct cgccgaaccc cttggccgat
    11701 agccgcacga caaatcccgg tatgccgccg tcgcgcacca aaagattgga accgatgccg
    11761 accatcgtca gcggcacctc ttgcggcacc gccttcagga aggcggcaag gtcgtcctcg
    11821 tcggccggct ggaacagcgc gtcggccagc ccgccggcgc gaaaccaggt gattttgtcc
    11881 atctccgcgt tgggcgtgat gcgcccgcgc aggccggcaa gctggtcgcc cagcgaggca
    11941 agaagctgct cgccgcgcgt catgatccat tcagttccct gggcagcgca taggcccatt
    12001 gcgtgatgct gccggcgcca aggaacacga cgaagtcgcc cggcccggcc agcccgcgta
    12061 cgatcggcgc tatcgcttcc ggaccctcga tataacgggc gtcgcgatgg ccgccggcgc
    12121 gcagccgcgc caccaatgca tcggaagtcg cgccttcgat ggggtcttcg ccggctgtat
    12181 agaccggcgc gatcatcacc gtatcggcgt cgttgaagca cgaggcgaaa tcgtcgaaca
    12241 ggtcgtgcag ccgggtaaag cggtgcggct gcgcgatggc gatgacccgt cccttggttg
    12301 attcccgcgc tgccctgagc accgccgtga tctcgaccgg gtggtggccg taatcgtcga
    12361 acacatcgac gccgttccac gaacccgtat gggtaaaacg gcgcttgacg ccaccgaagg
    12421 acgacaggcc cttcttgatc gcctccggcg aaagccccag ctcatgcgcc acggcgatcg
    12481 cggcggtcgc attggagaca ttgtgccggc cgggcatcgg caggcgcagc ccgccaatct
    12541 cggtttgcct tcccttgcgg ttgcggatca ccacgtcgaa ctccgacgaa gcgccgttca
    12601 tgcgatggtt ggtgaaacgc acatcggcct gcgcgttcgc gccataggtg atgacacgcc
    12661 ggtcctcgat gcggctgacc agcgcctgca cttccggatg gtcggtacac atgacgccga
    12721 agccgtagaa cggcacgttc tcgacaaatt gccgaaacgc ctcgcgcacc gcgtcgaacg
    12781 aaccgtaatg atcgagatgt tcggggtcga tattggtgac cacggcaata tccgccggca
    12841 atttcaggaa tgtgccgtcg ctctcatcgg cctcgaccac catccattcg ccgtcgccca
    12901 tgcgcgcatt ggtgccatag gcattgatga tgccgccatt gatgaccgtc gggtccagcc
    12961 cgccagcctc cagcagcgtt gccaccatgg aagtggtcgt cgtcttgcca tgcgtgccgc
    13021 caatggccac cgcctggcga aagcgcatca gttcggccag catttcggcg cggcgcacga
    13081 tcggcagcag cctttcgcgc gcggctttca attcggggtt ggatttcttg atggcagtcg
    13141 agacgaccac cacctcggca tcgccgagat tttcagcggc gtggccgacg aagcattcga
    13201 tgcccttgtc gcgcagccgc tgcacattgg cgccgtcggc ctggtccgaa ccctgcacct
    13261 tgtagcccag attgtgcagc acctcggcga tgccgctcat gccgatgcca ccgatgccga
    13321 tgaagtgcac aagccctatc gtctgcggca ttttcatggg cgcatctctt tcttgaagtc
    13381 ggctgctgat ttgcccgacg caatagcctc tgtcaggtcg gcgagcaaca gcgtggcgtc
    13441 gcgccgcccg acgcttcgtg cccctgacgc catcgaagcc agccgatcgc cgtcggcaag
    13501 cagcgatcca agttctgcgg cgagcgcttc gacacccagg tcaccttggc gtcgcatgac
    13561 ggcgccgccg gcatcgacca ggatttgagc gttgtggccc tggtcgtcat caagcgcgtg
    13621 cggcaacggc acaaggatgg ccggccgccc gatcaccgac agttccagaa cggttgacgc
    13681 gcccgagcgg gcgacgacaa gctgcgcggc cgccatgcgc gccggcagat ccgcgaagaa
    13741 gggcgccacc tcggcttcca cgccgagttc gccatagcgc gcctttacgc gcgcctcgtc
    13801 ctcgctgcgc gcctgctgga cgatgcgcaa ctgctggcgc aacgtcgccg aaagcacggc
    13861 aatcgcctcg ggaacggtat cggaaaaata acgcgcgccc tggctgccgc caaaaatcaa
    13921 cagccgcacc ggcccatctg cttgtggccg ctcatatggc gtgtcggcca ctttcagcac
    13981 cgcctggcgt accggattgc ctgtctcggc gatctttctc gcataaggcc cggcagcctt
    14041 caggaagccg ccggcaatcg ccgtcacgcg cggtgcgagc gccttgttcg cgcgccccat
    14101 cacggcgttc tgctcatgaa cgagcgtggg aaccctgcgc cgcgtcgcgg caaaaagcgg
    14161 cggcagcgtc ggatagccgc cgaagccgat aaccaccttc ggcttcaact tgccgatcag
    14221 cgtggaagat tgccggaaac cccgccagag cgtccatagg ctgcgcagc
//
LOCUS       DASYCS010000013         4688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2321165,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000013 DASYCS010000000
VERSION     DASYCS010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4688
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2321165"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            274..1479
                     /locus_tag="VGM46_00535"
     CDS             274..1479
                     /locus_tag="VGM46_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEY4191108.1"
                     /translation="MAAEKNAFVWDDPFLIEGQLSEDERMIRDAAAAFAADKLAPGIE
                     EAYMEEKTDPSIFREMGTAGLLGITIPEEYGGLGSNYVTYGLVAREIERIDSGYRSMM
                     SVQSSLVIYPIHAYGSEAQRRKFLPKLASGEWIGCFGLTEPDAGSDPGGMKTRAEKTA
                     TGYRISGSKMWISNAPIADVFVVWAKLSNGSGGAGDNAIRGFILEKGMKGLSAPKIGG
                     KLSLRASITGEIVMENVEVGEDALLPNVSGLKGPFGCLNRARYGISWGAMGAAEDCWH
                     RARQYGLDRKQFGKPLAGTQLFQKKLADMQTEIALGLQGSLRVGRLMDEGKMAPEMIS
                     IVKRNNCGKALDIARQARDMHGGNGIQIGYHVMRHAQNLETVNTYEGAHDVHALILCR
                     AQTGLQAFF"
     gene            1730..2812
                     /locus_tag="VGM46_00540"
     CDS             1730..2812
                     /locus_tag="VGM46_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HEY4191109.1"
                     /translation="MLDKPRPPAARSNTSPENGNEAAARRASLVYVSDTDPGIRRKGS
                     PRCFDYVSPSGKSLKEGRDMRRIRALAIPPAWIDVWISPDADGHIQATGRDARGRKQY
                     RYHADWAAERDGAKYSSLTAFAEALPRLRRQIETDLRRHGLPQERVVASIVWLLDNTM
                     IRIGNAAYARDNKSFGLTTLRDRHVDIDGSKLRFSFKGKSGKEWKLKLTDRRIARVVR
                     GAQDLPGQKLFQYLDDEGGRHPVRSEDVNRYIHDALGDGFSSRHFRTWGGTIRAASLF
                     AQTELPEGKTAQARVMNSVIDKVAEQLNNTRAVCRKCYIHPAVFEAWSQGALLEQMTA
                     ASKRRRSISGLDDEEAIVLRWLKSID"
     gene            2913..3290
                     /locus_tag="VGM46_00545"
     CDS             2913..3290
                     /locus_tag="VGM46_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEY4191110.1"
                     /translation="MLYAILCYASEDVVSGWSKAEDDAVMEKLAAVQERYAKAGKLGP
                     VARLLPTTAATTLRKVRGESVVIDGPFAETKEQFLGFYTVDCDDLEEAVGFARELSEV
                     NPSGGSYEIRPVSLFSPVKATIR"
     gene            3287..4561
                     /locus_tag="VGM46_00550"
     CDS             3287..4561
                     /locus_tag="VGM46_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY4191111.1"
                     /translation="MSDLGWISSAISAARPQAVAALLRYFRDLDTAEEAFQDACLRAL
                     KNWPQNGPPRDPAAWLIFVGRNSGIDAVRKRAKQVPMPEEELVSDLEDAEGDMAERLD
                     GAHYRDDILRLLFICCHPDLPATQQIAVALRIVSGLTVKQIARAFLVGESAMEQRITR
                     AKARIAEAGTAFETPGPVERSERLAAVAAMIYLVFNEGYSAGSGEASARAPLCEEAIR
                     LCRLLLRLFQSEPEIMGLTALMLLQHARAPARFDADGQIVLLEDQDRSLWSAKMIGEG
                     LALVDKALRHAKPGPYQVQAAIAALHARAAKAEDTDWQEIELLYGLLERMQPSPVVTL
                     NRAVAVAKVQGPQAALAMVEPLEDRLSGYFHFFGLKGGLLMQLGRGEEARIAFDRAIA
                     LANTAAEAAHIRLHIDRLMKAAPPRGNAKAVV"
BASE COUNT          866 a         1404 c         1570 g          848 t
ORIGIN      
        1 tgcgcaagga tggtggcggc gaggcaaggc ccgctccgcg cgagccggcc ggcaagcctc
       61 gcgcggcacc ggcttcgcgc gcgccggaac ggccggcggc gcagggagcg ttcggcacgg
      121 cgctggcgga cgcgatgaaa cggaaatagc ctcggacttg cggggcaata ccgcgcgggt
      181 tccgggttaa gcggattggc tggcggattg gtttgatttt ttctacggcg cgtcggataa
      241 gccgggcaat tcacggactt tggaggaaaa atcatggctg ccgaaaagaa cgccttcgtc
      301 tgggacgatc ctttcctgat cgagggccag ctttcggaag acgagcggat gatccgcgat
      361 gcggcggcgg ctttcgccgc cgacaagctc gcgcccggca tcgaggaagc ctatatggag
      421 gagaagacgg acccgtcgat cttccgcgaa atgggcaccg ccggcttgct cggcatcacc
      481 attcccgagg aatatggcgg ccttggcagc aattacgtga cctacgggct ggtggcgcgg
      541 gagatcgagc ggatcgattc cggctatcgc tcgatgatga gcgtgcagtc gtcgctggtg
      601 atctatccga tccatgccta tggctcggag gcacagcgcc ggaaattcct gccgaagctt
      661 gcttccggcg aatggatcgg ctgcttcggc ctgaccgagc cggatgccgg ttccgatccg
      721 ggcggcatga agacgcgcgc ggaaaagacc gcgaccggct acaggatttc cggctccaag
      781 atgtggattt ccaacgcccc gatcgccgac gttttcgtcg tgtgggcgaa gctttccaat
      841 ggatccgggg gtgcgggtga caatgcgatc cgtggcttca tcctggaaaa gggcatgaag
      901 gggctttccg cccccaagat cggtggcaag ctttcgttgc gcgcgtcgat taccggtgag
      961 atcgtgatgg aaaacgtcga ggtgggcgag gatgcgctgc tgcctaatgt ttccggcctc
     1021 aagggaccgt tcggctgcct caatcgtgcg cgttacggca tttcctgggg cgcgatgggc
     1081 gcggctgaag attgctggca ccgcgcccgc cagtatggcc tcgaccgcaa gcagttcggc
     1141 aagccgctgg ccggcacgca gcttttccag aagaagctcg ccgacatgca gaccgaaatc
     1201 gcgcttggcc tgcaaggctc gctgcgcgtc gggcggctga tggacgaggg caagatggcg
     1261 ccggagatga tctccatcgt caagcgcaac aattgcggca aggcgctcga cattgcgcgg
     1321 caagcgcgcg acatgcatgg cggcaacggc atccagatcg gttaccacgt catgcgccac
     1381 gcgcagaacc tagaaacggt caacacctat gagggcgcgc atgacgtgca cgcgctgatc
     1441 ctctgccgcg cgcagaccgg tttgcaggct ttcttttgag cgatgacaaa aagattcggg
     1501 atgcagcgct aattcattgg tttcgtttcg gatttttgcc tgaaatcaga cccgccgatt
     1561 tcggtcccgg ttgcaggtgc gcgctgacaa agtgaatcac gtggcagcgc caacttcctg
     1621 tttatgaatc gaattcggac aggtgcgcgg ccccgtgctt ttcattggag ggtgcggaac
     1681 cagccggcca ccagcttctt atctacggac tgatttccgt ggagcgaaca tgctggacaa
     1741 gccccggccg ccggccgccc gatcaaatac atcgccggaa aacggcaatg aggcggcggc
     1801 gaggcgcgct tcgctggttt atgtcagcga caccgatccc ggcattcgcc gcaagggctc
     1861 gccaaggtgt ttcgactatg tctctccctc cgggaaatcg ctcaaggaag gccgggatat
     1921 gcggcgtatc cgggcgctgg cgataccacc ggcctggatc gatgtgtgga tttcgccgga
     1981 cgccgacggc catatccagg cgacgggacg cgatgcgcgc gggcgcaagc aataccgcta
     2041 ccacgccgac tgggcggctg aacgcgacgg cgccaaatat tcaagcctga ccgcctttgc
     2101 cgaggctctg cctcgtcttc gccggcagat cgagaccgac ctgcggcggc acggccttcc
     2161 acaggagcgg gtggtggctt ccatcgtctg gctgctagac aacacgatga tccgcatcgg
     2221 caacgctgcc tatgcgcgcg acaataagag cttcggcctg acgacactgc gcgaccgcca
     2281 tgtcgacatt gacgggtcga agctgcgctt ttcgttcaag ggcaagtccg gcaaggaatg
     2341 gaagctgaag ctgaccgatc gtcgcattgc ccgcgtggtg cgcggggcgc aggatttgcc
     2401 gggacagaag cttttccaat atctggatga cgaaggcggc cggcacccgg tccgctcgga
     2461 ggatgtcaac cgctacattc acgacgcgct tggcgatggt ttcagctcca ggcattttcg
     2521 cacctggggg ggaactatcc gcgccgcctc gctgtttgcg cagacagagt tgccggaggg
     2581 caagacggcg caggcgcgcg tcatgaattc ggtcatcgac aaggttgccg aacagctcaa
     2641 caacacgcgc gcggtctgcc gcaaatgcta tatccatcca gccgtcttcg aagcctggtc
     2701 gcagggtgcg ctgcttgagc agatgaccgc cgccagcaag cgcaggcgct cgatttcagg
     2761 tctggacgat gaggaagcca tcgtattgcg ctggctcaaa tccattgatt gagatgccgc
     2821 tgccgattgc gggcgaggtt tgtgaaaaat cggtcgacgc catgtcgggt tcgcccttac
     2881 tctttcgtcc tttgacgaaa ggaaggaacg acatgctcta cgccattctt tgctatgcct
     2941 ccgaagacgt tgtcagcggc tggtccaagg ccgaagacga tgcggtgatg gaaaagctgg
     3001 ccgccgtgca ggagagatac gccaaggcag gaaaactcgg cccggtggcg cggctattgc
     3061 cgaccacggc tgcaacgacg ctgcgcaagg tgcgcggtga atcagtggtt atcgatgggc
     3121 cgttcgcgga aaccaaggaa cagttcctcg gcttctatac ggtcgactgc gacgatcttg
     3181 aggaagctgt cggcttcgcc cgcgaattgt cggaggtcaa cccaagcggc ggctcgtatg
     3241 aaatacgccc cgtcagcctg ttcagtccgg taaaggcgac cataagatga gcgatctcgg
     3301 ctggatcagt tccgcgatca gcgccgcgcg cccgcaggcg gtggcggcgc tgctgcgcta
     3361 ttttcgcgat ctcgatacgg ccgaagaagc attccaggac gcctgcctgc gcgctctcaa
     3421 gaactggccg cagaacggcc cgccgcgcga tcccgccgcc tggctgattt tcgtcggccg
     3481 caacagcggc atcgacgccg tgcgcaagcg cgccaaacag gtgccgatgc cggaagagga
     3541 gctggtgtcc gacctggagg acgccgaggg cgacatggcg gagcggctgg acggcgcgca
     3601 ttaccgcgac gacatattgc ggctgctgtt catttgctgc catcccgatc tgcccgccac
     3661 gcagcagatc gcggtggcgc tgcgcattgt ctcgggcctg accgtcaagc agatcgcgcg
     3721 cgcgtttctg gtcggcgaaa gtgcgatgga gcagcgcatc acccgcgcca aggcgcgcat
     3781 tgccgaagcc ggaacggctt tcgagacgcc ggggccggtg gagcggtccg agcggctggc
     3841 ggcggttgcg gcgatgatct acctcgtctt caacgagggt tactcggcgg gtagtggtga
     3901 ggcttcggcc cgcgcgccct tgtgcgagga agcgatccgg ctttgccgcc ttttgctgcg
     3961 cctgttccag agcgaaccgg aaatcatggg gttgacggcg ctgatgctgc tgcaacatgc
     4021 gcgcgccccg gcgcgtttcg acgccgacgg ccagatcgtg ctgctggaag accaggaccg
     4081 cagcctgtgg agcgccaaga tgatcggcga ggggctggcg ctggtcgaca aggcgctgcg
     4141 ccatgccaag cccggcccct atcaggtgca ggcggcaatc gcggcgcttc atgcgcgggc
     4201 ggcgaaggcc gaggataccg actggcagga gatagagctg ctctacggcc tgctggaacg
     4261 gatgcagccg tcgccggtgg tgacgctcaa ccgggcggtt gccgtggcca aggtgcaggg
     4321 gccgcaggcg gcgcttgcca tggtcgagcc gctggaagac cggctgtcgg gctatttcca
     4381 tttcttcggc ctcaagggcg ggctgctgat gcagcttggc cgcggcgagg aagcgcgcat
     4441 cgccttcgac cgggcgatcg cgctggccaa cacggccgcc gaggccgcgc atatccgcct
     4501 gcatatcgac cggctgatga aagctgcgcc gccgcgcggc aatgccaagg cggtcgtcta
     4561 agcgcctgcc atcgcaaccc gtattctcgc tggcacaatg tctgcccggc cgactggcgg
     4621 cgcgccgggg tggcggattt cgtctgaaac ggctaatgcg aagatcatga cagccctgca
     4681 agccttgg
//
LOCUS       DASYCS010000014         6431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3046415,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000014 DASYCS010000000
VERSION     DASYCS010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 6431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6431
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3046415"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..967)
                     /gene="hemA"
                     /locus_tag="VGM46_00555"
     CDS             complement(<1..967)
                     /gene="hemA"
                     /locus_tag="VGM46_00555"
                     /EC_number="2.3.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012090709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003870 - 5-aminolevulinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-aminolevulinate synthase"
                     /protein_id="HEY4191112.1"
                     /translation="MNYQRFFEDAIDQLHAERRYRVFADLERIVGKFPRAIWRANGRA
                     QEITVWCSNDYLGMGQHPDVIAALQNAAGQMGSGAGGTRNISGTSNPLVELEMELADL
                     HGKEAGLAFTSGFISNEASISTIARLLPNCLIISDELNHASMIEGVRRSGAEKKIFRH
                     NDVAHLESLLQAAGRERAKLIVFESVYSMDGDIAPIEKIADLADKYNAMTYIDEVHAV
                     GMYGPRGGGITEREGLADRIDIIEGTLAKAFGTLGGYITGTRAVIDAVRSYAPGFIFT
                     TALPPALAAATTVSIRHLKNSQAEREAQQRQVAHTKHVLAAANLPV"
     gene            1216..2211
                     /gene="galE"
                     /locus_tag="VGM46_00560"
     CDS             1216..2211
                     /gene="galE"
                     /locus_tag="VGM46_00560"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEY4191113.1"
                     /translation="MTVLVTGGAGYIGSHMVWELLDAGEDVIVLDRLSTGFEWAVAPE
                     AELVVGDVADRELVAGIIRQRKVDAIIHFAGSIVVPESMADPLAYYENNTAKTRTLIE
                     TAVREGVPNFIFSSTAAVYGGAGQEPVREDARLAPESPYGLSKLMSEWMLRDAAAAHG
                     LHYTALRYFNVAGADPQGRTGQSTPGATHLIKVACETALGKRPSMQVFGNDYDTPDGT
                     CVRDYIHVSDLAAAHRLALQRLRGGGANMVANCGYGHGYSVLEVIDSVKRAFGHEFAI
                     TAGPRRPGDAAAIVANCDLARRELGWQPKLDDLDRIVADALSWERILTAKNSSRG"
     gene            complement(2218..3204)
                     /locus_tag="VGM46_00565"
     CDS             complement(2218..3204)
                     /locus_tag="VGM46_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012067692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY4191114.1"
                     /translation="MFRWGVLSTAKIGHDHLIPAIVDAQNGVLSAIASRDPGKAQALA
                     ERFGARHAFGSYEELLASPEVDGVYIPLPTSQHVEWAARAMEAGKHVLVEKPLALDAK
                     DILPLIKLRDEKKLLVCEAFMVVYHPQWIKVRELIASGAIGRLRHVQGAFSYYNVDPD
                     NMRNQLDLGGGALPDIGVYPTVSTRFVTGREPLRVQATIERDKKFGTDVYSSIRADFG
                     DFELSFYLSTQMAARQSMVFHGEKGFIEMASPFNAGLYDHHRIELHDQNHAEAQIFRF
                     PDTRQYRLEVEAFARAALGGKDRVFTLEESVLNQKVIDAIFRAGGKDGWEAV"
     gene            3333..4328
                     /locus_tag="VGM46_00570"
     CDS             3333..4328
                     /locus_tag="VGM46_00570"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY4191115.1"
                     /translation="MKARKLGTELSVFPVGLGCMGMSHGYGGQEEADAIRTLHRAVEI
                     GVNFFDTAEVYGPHTNEILVGKAFRGMRDKVNIATKFGFNVVDTPEGPKTLPGLNSKP
                     GHVRAAAEGSLKRLGVEVIDLLYQHRVDPEVPIEDTIGEMAELVREGKVRALGLSEVS
                     AATIRKAHAVHPIAAVQSEYSLWTRDPEADVLETCRELGVGFVPFSPLGRGFLAGAIK
                     SADQLGEKDFRRGLPRFEAENIEKNLAAVARLEKLAAAKGASAAQLALAWVLHQGDFI
                     VPIPGSRKIANLEQNVAAADISFSPAELDEIGSVITPDSTAGQRYGAAMMAQTNR"
     gene            complement(4370..>6431)
                     /gene="pheT"
                     /locus_tag="VGM46_00575"
     CDS             complement(4370..>6431)
                     /gene="pheT"
                     /locus_tag="VGM46_00575"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEY4191116.1"
                     /translation="HGMMCSERELQLSDEHNGIIDLPQDAPVGASFAAYAHLDDPVID
                     INLTPNHPDATSVHGIARDLAASGLGRLRTAPVEPVAGKGECPVKVTIEAPELCPGFA
                     LRLVRGVKNGPSPKWMQQRLIAIGLRPISALVDITNYVTFDRGRPLHVFDAAKVAGNL
                     AVRRAREGEKVLALDGREYALTPEMCVIADENGVESIAGVMGGEHSGCDENTTDVLIE
                     SALWDPITTARTGRSLGIITDARYRFERGVDPEFMVPGLELATKLVLDLCGGEATKSE
                     VVGYAGHTPKTVSLPLSEVKRLTGLDVAPEKILNILSRLGFQPKGSGDPVEVAVPSWR
                     PDVDGKADLVEEVMRMHGVDQIIPQPLASHDAVNGKILTVLQIRTRAARRALAVRGMM
                     EAVTWSFIPAKHAELFGGGQPGLKLANPIAADMSDMRPSLLPGLVAAAQRNADKGIGD
                     VALFEVSGTYEGDAPENQRRVAAGVRRGTARLEGSGRNWAGNAPSVGVFDAKADALAA
                     LEACGAPVDRLQIETGAPSWYHPGRSGVIKLGPKVVLGHFGEFHPRTLEGLDVSGPLC
                     GFEIFVDSVPEPKAKPTRTKPKLELSSFQAVKRDFAFVVGKEVEAGALTKAALAADKK
                     LINGVSVFDVFEGASLGADKKSIAIEVSIQPMEKTLTDEDFEALAKRIVDNVAKQTGG
                     VLRG"
BASE COUNT         1140 a         2043 c         2106 g         1142 t
ORIGIN      
        1 ttaccggcag attggccgca gccagcacat gcttggtatg ggcgacctgg cgctgctgcg
       61 cttcgcgctc ggcctgcgaa ttcttcaaat ggcggatgga aacggtggtc gcagccgcaa
      121 gcgccggcgg caaggcggtg gtgaagatga agcccggcgc ataggagcgc acggcgtcga
      181 tgaccgccct ggtgccggtg atgtagccgc ccagcgtgcc gaacgccttg gccagcgtgc
      241 cctcgataat gtcgatgcgg tcggccagcc cctcgcgctc ggtgatgccg ccgccgcgcg
      301 ggccgtacat gccgacagcg tgaacctcgt cgatataggt catggcgttg tatttgtcgg
      361 ccaggtcggc gattttctcg attggcgcga tgtcgccgtc catcgaatag acgctctcga
      421 agacgatcag cttggcgcgc tcgcgccccg ccgcctgcaa aaggctttcc aggtgcgcga
      481 catcgttgtg gcggaaaatc ttcttctccg cgcccgagcg gcgcacgccc tcgatcatcg
      541 aggcatggtt cagttcatcg gagatgatca ggcaattcgg caaaagccgc gcgatggtcg
      601 agatcgacgc ctcgttggaa atgaagccgg aagtgaaggc aaggccggcc tccttgccgt
      661 gcaggtcggc cagttccatt tccagttcca cgagcggatt gctggtgccg gaaatgttgc
      721 gcgtgccgcc ggcgcccgaa cccatctggc cggctgcgtt ctgcaaggcg gcgatgacat
      781 ccgggtgctg gcccatgccg agatagtcgt tggagcacca gacggtgatt tcctgcgcgc
      841 ggccattggc gcgccagatg gcgcggggaa actttccaac gatgcgctcg agatcggcga
      901 agacccggta gcgccgctcc gcgtggagct ggtcgatggc atcctcgaag aaccgctgat
      961 agttcatgct gacgtcccaa aattttgcgc ggatcataac cactggccgt ttgcgcgtcc
     1021 accggacggt tttggtcaaa atgccacgcc ctttccgctt cgccaaagac ggcaggggcc
     1081 tgtccgctgc ccggtcttgg acaagtccgc cgctgcatgg caggctgcct ccatgcgatt
     1141 ttaacgggtt tgcgccaaga gtgcagcagg ccggcgattg aggcagtttc cttcataaca
     1201 ggggtattcg ggacaatgac ggttctggtg acgggcggtg ctggttatat cggcagccac
     1261 atggtgtggg aactgctgga tgcgggcgag gacgtcatcg tgctcgaccg cctctccacc
     1321 ggcttcgaat gggcggtggc gccggaggcc gaactggtgg tcggcgatgt cgccgaccgg
     1381 gaactggtcg ccgggatcat ccggcagcgc aaagtcgatg cgatcatcca tttcgccggg
     1441 tcgatcgtcg tgccggaatc gatggccgat ccgctcgcct attacgagaa caacacggcc
     1501 aagacccgca cgctgatcga aacggcggtg cgcgagggcg tgcccaactt catcttctcc
     1561 tcgaccgccg ccgtttatgg cggcgcagga caggaaccgg tgcgcgagga tgcgcggctt
     1621 gcgccggaat cgccctatgg cctttccaag ctgatgagcg agtggatgct gcgcgatgcg
     1681 gccgccgcgc acggcctgca ctatacggcg ttgcgctatt tcaacgttgc cggcgccgac
     1741 ccgcagggcc gcaccggcca gtcgacgccg ggcgccaccc acctcatcaa ggtcgcctgc
     1801 gaaacggccc ttggaaaacg cccttccatg caggtgttcg gcaacgacta cgacacgccg
     1861 gacggcacct gcgtgcgcga ctacatccat gtcagcgatc tggcggcagc ccaccggctt
     1921 gccttgcagc ggctgcgcgg cggcggcgcc aacatggtgg ccaattgcgg ctatggccac
     1981 ggttattcgg tgctcgaagt gatcgacagc gtgaagcgcg ccttcggcca tgaattcgcc
     2041 attaccgccg gtccgcgccg gccgggtgac gcggcggcca tcgtcgccaa ttgcgatctg
     2101 gcgcggcgcg aactgggctg gcagcccaag ctggacgatc tcgaccgcat cgttgccgac
     2161 gcgctgtcgt gggagcgcat cctgacggcc aagaattcgt cgcgcggctg accctgctta
     2221 gaccgcttcc cagccatcct tgccgccggc gcggaagatg gcgtcgatga ccttctggtt
     2281 gagcacggat tcctccagcg tgaagacgcg gtccttgccg ccaagcgccg cccgcgcgaa
     2341 cgcctcgact tccagcctgt attgccgcgt gtcgggaaag cggaagattt gtgcctccgc
     2401 gtggttctgg tcgtgcaact cgatgcggtg atggtcgtag aggccggcat tgaacggcga
     2461 ggccatctcg atgaaaccct tttcgccatg aaacaccatg gattggcgcg ccgccatctg
     2521 ggtggacaga taaaaggaca gttcgaaatc gccgaaatcg gcgcggatcg aggaatagac
     2581 gtcagtgccg aatttcttgt cgcgctcaat ggtcgcctgc acccgcagcg gctccctgcc
     2641 ggtgacaaag cgcgtcgaaa cggtggggta gacgccgata tcgggcagcg cgccgccacc
     2701 cagatcgagc tggttgcgca tgttgtccgg gtcgacattg tagtatgaaa acgcaccctg
     2761 cacatggcgc agccggccta tcgcgccgga agcgatcagt tcacgcacct tgatccattg
     2821 cgggtggtag acgaccatga aggcttcgca gacgagcagc tttttctcat cgcgcagctt
     2881 gatgagcggc agaatatcct tggcgtcgag cgccagcggc ttttccacca gaacatgctt
     2941 gccggcttcc atcgccctgg ctgcccattc gacgtgttgt gaagtcggca gcggaatata
     3001 gacgccgtcg acttccggcg aggcgagaag ctcctcatag gagccgaagg cgtggcgggc
     3061 gccgaaacgt tcggccaagg cctgcgcctt gccggggtcg cggctggcga tggccgaaag
     3121 cacgccgttt tgcgcatcga caatggcagg gataaggtgg tcgtggccga tcttggcggt
     3181 cgataaaaca ccccagcgaa acatccgcac cactccttcg attgtgatgc gccaagcttt
     3241 gcccgaaaag ccggactgtg ccaagttgcg cccacatcga ctcaaaccct tcgcgggcga
     3301 aacctcgcct gcaatttccc aaaggaaccg ccatgaaggc ccgcaagctt ggaaccgaac
     3361 tgtccgtctt tcccgtcgga ctgggctgca tgggcatgag ccacggctat ggcggccagg
     3421 aagaggccga cgccatccgc acgctgcacc gcgcggtcga gatcggcgtg aacttcttcg
     3481 atacggcgga agtctatggg ccgcatacga acgaaatcct agttggcaag gcgtttcgcg
     3541 ggatgcgcga caaggtaaac atcgccacca aattcggctt caacgtggtc gatacgccgg
     3601 aggggccgaa gacattgccc ggcctgaaca gcaagcccgg tcatgtccgc gccgccgccg
     3661 agggctcgct taagcggctc ggcgtcgagg tgatcgacct gctctaccag caccgcgtcg
     3721 atcctgaagt gccgatcgaa gacacgatcg gcgaaatggc cgaactggtg cgcgagggca
     3781 aggtacgggc gctgggcctt tccgaagtca gcgcggccac gatccgcaag gcgcacgccg
     3841 tgcatccgat cgcggcggtg cagagcgaat attcgctgtg gacgcgcgac cccgaggcgg
     3901 acgtgcttga aacgtgccgg gaactgggcg tcggcttcgt gccgttcagc ccgctgggac
     3961 gcggcttcct cgccggcgcg atcaagtccg ccgaccagct tggcgaaaag gatttccggc
     4021 gcggcctgcc gcgcttcgag gccgagaata tcgagaagaa cctggccgcg gtggcgcggc
     4081 tggaaaagct ggccgcagcc aagggcgcca gtgcggccca gcttgcgctg gcctgggtgc
     4141 tgcaccaggg cgacttcatc gtgccgatcc ccggttcgcg gaagatcgcc aatctggaac
     4201 agaacgtggc ggcggcggat atttcgttca gcccggccga gcttgacgaa atcggcagcg
     4261 tgatcacgcc cgacagcacc gcgggccaac gctatggcgc ggcgatgatg gcgcaaacca
     4321 acaggtgagt tgcgggggcc ggagcgtttc ccgacaggat ggaaacgctc tagccccgca
     4381 gcacgccgcc ggtttgcttc gcgacattat cgacgatgcg cttggccagc gcctcgaaat
     4441 cctcgtcggt cagcgtcttt tccatcggct ggatcgacac ttcgatggcg atggacttct
     4501 tgtcggcgcc aagcgacgcg ccttcgaaca cgtcgaacac cgaaacgccg ttgatgagct
     4561 ttttatcggc ggccagtgcc gccttggtca gcgcgcccgc ctcgacttcc ttgccgacga
     4621 cgaaggcgaa atcgcgcttc accgcctgga agctggaaag ctccagtttc ggcttggtcc
     4681 gcgtcggctt ggccttcggc tcgggcacgc tgtcgacgaa gatttcaaag ccgcagagcg
     4741 ggccggaaac gtccagccct tccagcgtcc tcggatggaa ttcgccgaaa tggccgagca
     4801 cgactttcgg cccgagcttg atcacgccgg agcggccggg atgataccag gagggcgctc
     4861 cggtctcgat ctgaagccgg tccactggcg cgccgcaggc ttcgagcgcc gccagcgcat
     4921 cggccttggc gtcgaacacg ccgacgcttg gcgcattgcc ggcccagttg cggccggagc
     4981 cttcgagcct ggcggtgccg cgccgtacgc cggccgccac gcggcgctgg ttttcgggcg
     5041 catcgccttc ataggtgcct gaaacctcga acaaggcaac gtcgccaatg cccttgtcgg
     5101 cattgcgttg cgccgccgcg accaaccccg gcaacagcga cgggcgcatg tcggacatgt
     5161 cggcggcgat cgggttggca agcttgagcc ccggctggcc gccgccgaac agttcggcat
     5221 gtttcgccgg aatgaacgac caggtgacgg cctccatcat gccacgcacg gccagcgcgc
     5281 ggcgcgcggc gcgggtgcgg atttgcagca cggtgagtat cttgccgttg acggcgtcgt
     5341 ggctggcgag cggctgcgga atgatctggt cgacgccgtg catgcgcatg acctcctcga
     5401 ccagatcggc cttgccgtcg acgtccggcc gccatgacgg cacggcgacc tcgaccggat
     5461 cgccggagcc tttcggctgg aagcccaggc gtgaaaggat gttcaggatc ttctcaggcg
     5521 caacgtcgag gccggtcagc cgcttcactt ccgacagcgg caaagagacg gtcttgggcg
     5581 tgtggccggc atagccgacc acttcgcttt tcgtggcttc gccgccgcac agatcgagca
     5641 ccaactttgt cgccagttcc agccccggca ccatgaattc aggatcgacg ccacgctcga
     5701 agcggtagcg cgcatcggtg atgatgccga gcgaacggcc ggtgcgggcc gtcgtgatcg
     5761 ggtcccacaa cgcggattcg atcagcacgt cggtggtgtt ttcgtcacag ccggaatgct
     5821 cgccgcccat gacgccggca atggattcga cgccgttttc atcggcgatg acgcacattt
     5881 caggcgtcag cgcatattcg cggccatcca gcgccagcac cttctcgcct tcgcgcgcac
     5941 ggcgaacggc gaggttcccg gcgaccttgg cagcgtcgaa cacatgcagc gggcggccac
     6001 ggtcgaacgt cacatagttg gtgatatcga ccagcgcgct gatggggcgc aggccgatgg
     6061 cgatcaggcg ctgctgcatc catttcggcg acggcccgtt cttcacgccg cgcaccagcc
     6121 gcagcgcaaa accggggcaa agctccggcg cttcgatggt gaccttgacc gggcattcgc
     6181 ccttgccggc caccggctcg accggagcgg tcctgaggcg accaaggccc gaagccgcca
     6241 ggtcgcgcgc aatgccgtgg acgctcgtgg cgtccggatg gttcggcgtc aggttgatgt
     6301 cgatgaccgg atcatcgaga tgggcatagg cggcgaagct ggcgccgacc ggcgcgtcct
     6361 gcggcaggtc gatgatgccg ttatgctcgt cggaaagctg aagctcgcgc tccgagcaca
     6421 tcatgccgtg g
//
LOCUS       DASYCS010000015         9589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1451753,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000015 DASYCS010000000
VERSION     DASYCS010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 9589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9589
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1451753"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VGM46_00580"
     CDS             complement(<1..221)
                     /locus_tag="VGM46_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006199689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid aminotransferase"
                     /protein_id="HEY4191117.1"
                     /translation="MTLATAAQSTTWTFVDGDWHEGNVAILGPRSHAMWLGTTVFDGG
                     RWFEGVSPDLDLHARRVNASAIALGLKPT"
     gene            389..1051
                     /locus_tag="VGM46_00585"
     CDS             389..1051
                     /locus_tag="VGM46_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006330944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEY4191118.1"
                     /translation="MQHAAYVFDAYGTLFDVHAAVRRHADVIGPDGPRMSELWRTKQL
                     EYSWIRTLMGTYADFWQLTEQALDFSLAKYPSIDASIRPKLLDAYLRMDCYPEVPAML
                     AALKASGARLAILSNGSPEMLDAAVKSCALDQVLDDVFSVDTVRRFKTDPAVYEMVTT
                     NWRLYPEAVSFQSSNRWDVAGAASFGFRTVWINRTNQPEEYSDFPPSLILPSLEGLAS
                     AG"
     gene            1231..2058
                     /locus_tag="VGM46_00590"
     CDS             1231..2058
                     /locus_tag="VGM46_00590"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEY4191119.1"
                     /translation="MTSSTHPFRPISRRGFLNTAALGAASFAVSACTTTGRQQPIEQA
                     PAYSEPPLGDYATMYGPVVDNGFAVPAIPIEKIDPQFLRQIVPDPTGEQPGTIVVDTT
                     GHFLYLVREGGQAIRYGVGLGRAGFEWSGDAVVRWKQKWPKWTPPDEMIARQPELTKY
                     SADNGGMPGGLKNPLGARALYLFQGDVDTLYRLHGSPEWNSIGKSVSSGCVRLMNQDI
                     IDLYDRVPNKSKVVVTSNVGRPMAANASSGQAIPIDDGVPEGSVLLGRVKSFSNSIF"
     gene            complement(2097..3635)
                     /locus_tag="VGM46_00595"
     CDS             complement(2097..3635)
                     /locus_tag="VGM46_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010914995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HEY4191120.1"
                     /translation="MADDKSIFLGASRKPDDSYQRAEELLLQYGNRHGLVTGATGTGK
                     TVTLQILAESFSNAGVPVFCADIKGDLSGISMKGTPQDFLVKRAEQVKLDPYEFQDFP
                     VIFWDLFGEQGHPIRATVSEMGPLLLSRLMDLSEAQEGVLNIAFKIADDEGLLLLDMK
                     DLQALLANMASRAVEIGARYGNVTKPSVGAIQRTLLVLEQQGAAHFFGEPALKIADIM
                     RTTRDGRGAINVLAADKLMMNPRLYATFLLWLMSELFEELPEIGDPEKPRLVFFFDEA
                     HLLFDDAPKVLLNRVEQVVRLIRSKGVGIYFVTQNPLDVPEMVLAQLGNRVQHALRAY
                     TPREQNAVKTAADTFRPNPDFDCATVITQLATGEALVSTLEAKGIPSMVQRTLIRPPS
                     SRLGPITPEERRKIILESPVAGQYDEAVDRESAFEILQKKTKAAQDAEQGAGEGGSRW
                     TIPGFGGDDANAPAQTGRRPPARASNRQTVAEAAIKSVVRSVGSSVGRAIVRGILGSL
                     SKGR"
     gene            3892..5319
                     /locus_tag="VGM46_00600"
     CDS             3892..5319
                     /locus_tag="VGM46_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase subunit beta"
                     /protein_id="HEY4191121.1"
                     /translation="MIRDSEFPAANRRQWVALAEKALGDTAFEDALVSRSDDAIPIDP
                     LHERAIDAAASLRVRPDLPWTISQRVDDPDVERAKAQIEEDLAQGATGLSLVFEGAPN
                     AFGYGLPATAQALESVLEGVPLNRIHLRIDVHPSSRAMADWLVGLLTRRRADPEKLNV
                     SFGIDPAATFAGTGRLRMSIEALQASMPQSLAHFFAMGVPGVLLEADGRVFHNSGATE
                     AQELGIMLASAVSHLRLFEEARQALIYAAPHIGFALSVDQNQFLSLAKVRALRALWAR
                     IQQTCSIPASDATIHAETSLRMMTALDPETNILRNTIACFAAAAGGADSISILPHTIT
                     HGLPAGFARRIARNTQLIMARESHIDHVNDPARGSGGVEALTAGLCEAAWAEFQRIEA
                     EGGVLASLREGHIQKRVAAARLERQKLYLANKRTIIGTTLYPAKKERPVETLAALKHA
                     GPTDGVVFCEPILPARLDQFAEADQ"
     gene            5316..7439
                     /gene="scpA"
                     /locus_tag="VGM46_00605"
     CDS             5316..7439
                     /gene="scpA"
                     /locus_tag="VGM46_00605"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HEY4191122.1"
                     /translation="MIPDFSKIAWSKAPALPRDVAGEWLTPEGIAIKGLYSEADIASL
                     PDLDTYPGIAPFMRGPYPTMYVQKPWTIRQYAGFSTAEESNAFYRRNLAAGQKGLSIA
                     FDLATHRGYDSDHPRVAGDVGMAGVAIDSILDMRQLFDGIPLDQMTVSMTMNGAVLPI
                     LALYIVAAEEQGVAQKDLTGTIQNDILKEFMVRNTYIYPPKPSMRIVSDIFSYTSRHM
                     PKFNSISISGYHMQEAGATADLELAYTIADGIEYARAGVAAGLDIDKFAPRLSFFWAI
                     GMNFFMEVAKLRAARLIWAQLMQKNFAPKDQRSLSLRTHCQTSGWSLTAQDPYNNIAR
                     TMIEAMAATQGHTQSLHTNSFDEALALPTDHSARIARNTQLVLQKESGTTAIIDPWGG
                     SAYVERLTHDLAERALAHIEEVERLGGMAAATEKGIPKLRIEEASARTQARIDSGDQI
                     LVGVNAYRPDSDTDVDVLKIDNAEVRARQLSKLQQLKGTRDVGAVESALDALTLAAKS
                     GENLLEFSIRAARANATVGEISLALEKAFGRHVASVQTISGVYRKALGETPAVGRLEE
                     KLAAFEKKAGSKPRILVAKMGQDGHDRGQKVIATAFADLGFDVTVGAMFQTPEEIAKL
                     AVEHNVHIVGASSLAAGHLTLIPELKDALEKSGHGDILVVAGGVIPPQDYDAVLKAGA
                     AEIFPPGTVIPEAAERLIDRLLAAT"
     gene            7509..7730
                     /locus_tag="VGM46_00610"
     CDS             7509..7730
                     /locus_tag="VGM46_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003547144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEY4191123.1"
                     /translation="MNTTVRKIGNSEGVILPKELLDRLNIKAGDELEIIATDKGVTLK
                     PVDDSFERQMKAARAMMDKYKVALQKLAE"
     gene            7727..8116
                     /locus_tag="VGM46_00615"
     CDS             7727..8116
                     /locus_tag="VGM46_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006330927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEY4191124.1"
                     /translation="MSWEFLSRRVVEAMHAEQLRRHGGAQGTRDENALESALARPQNK
                     AEYGEPTVHELTAAYVFGIARNHAFVDGNKRTAIVAAGTFLIVNGHTLTANDGTLYEF
                     VMGVAAGTIDEAGAAAFFRDHTVKIEG"
     gene            complement(8231..8701)
                     /gene="greA"
                     /locus_tag="VGM46_00620"
     CDS             complement(8231..8701)
                     /gene="greA"
                     /locus_tag="VGM46_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEY4191125.1"
                     /translation="MSRAFTREEDSENAIAGIGERPVSQHRNLVTQHGLAMIEAELAA
                     LRVELKNAESGADKQRIAMVSRDLRYWMARRESAELAVAEAGSGVVRFGMTVALESED
                     GSEVSWRIVGEDEADPAKGSISHVSPMARALFGKKIGDIAAVNGKEWEIVGLAG"
     gene            complement(8911..>9589)
                     /locus_tag="VGM46_00625"
     CDS             complement(8911..>9589)
                     /locus_tag="VGM46_00625"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018426940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEY4191126.1"
                     /translation="WPSSQAATVALAQGGGAPSALHNNCSGKHSGFLCTCVHAGIDHH
                     GYVEAGHAFQEMVRAAMEDVTGAAHNAANRGIDGCSIPTYAVPLRDLARGFARMVTGN
                     GLPPERAKAAGRLMSACMAEPFLISGTDKADAALMGAAPGRIFVKSGAEGVYWAAIPE
                     LGLGIALKCDDGAIRGAETMIAAVLAKLLASDEALSATLITQACPVLKNWNGLSVASL
                     RPTAALA"
BASE COUNT         1846 a         3077 c         2884 g         1782 t
ORIGIN      
        1 atggtcggct tgagaccaag cgctatcgcc gaggcgttga cccgcctggc atggaggtcg
       61 agatcgggcg agacgccctc gaaccagcgg ccgccgtcga acacggtggt gccgagccac
      121 atggcgtggc tgcgcggccc cagtatggcg acattgccct cgtgccagtc gccatcgaca
      181 aatgtccagg ttgtggattg cgccgccgtc gccaaagtca tgtattcgat ccgcctcaac
      241 gtgtcccggt acaccgggtg tcttgaagac attggaaagc cgtttgaggc gtgccgtcaa
      301 cgggcatgcg gcagttttaa tcgggccagc gcatatttgg ttgcaatccg ggcttgcttc
      361 ggcgacccac tcatcacaca aatgcgcaat gcaacatgcc gcttatgtct tcgatgccta
      421 cggaaccctg ttcgacgtgc acgccgcggt gcgccgacac gccgatgtga tcgggccgga
      481 cggcccccgc atgtccgaac tgtggcgcac caagcagctt gaatattcct ggatccgcac
      541 gttgatgggg acctatgccg atttctggca gctgaccgaa caggcgctcg atttttcgct
      601 ggccaaatat ccgtccatcg acgcctcgat ccggccgaag cttctcgatg cctatttgcg
      661 gatggactgc tacccggaag tgccggcgat gctcgccgcg ctcaaggcat cgggtgcgcg
      721 tttggcaatt ctatcgaacg gttcgcctga aatgctggat gcggcggtga agtcttgcgc
      781 gctcgaccag gtgctggacg acgtgttttc cgtcgatacg gtaaggcggt tcaagaccga
      841 ccccgccgtc tatgagatgg tgacgaccaa ctggcgtctt tatcccgaag cggtttcctt
      901 ccagtcctcg aacaggtggg acgttgcggg agcggccagt ttcggtttcc gcaccgtgtg
      961 gatcaaccgc accaaccagc ctgaagaata tagcgacttt ccgccgagcc tgatcctgcc
     1021 atcgctggaa gggctggcct cggccggctg agccattgtt gccaaacgga cacagtgaca
     1081 aggctgccac atcttggcct tgctttgtcc accttcaggc cagaatcgga accgcctatc
     1141 gccgctacag ttagcgggga gcatcgccag ccggcgcatg catgccggcg cattcatgct
     1201 ttcgtatgat tccaggaata aaaatatatt atgaccagtt ccacacatcc gtttcgccca
     1261 atcagccgtc gaggcttcct gaacacggca gcgcttggcg cggcgtcgtt cgccgtttcg
     1321 gcctgcacca cgaccggccg gcagcagcct atcgagcagg cgcccgctta ttccgaaccg
     1381 ccgcttggcg actatgcgac catgtacggc ccggtggtcg ataatggctt tgcggttccc
     1441 gcgataccga tcgagaagat cgacccgcag ttcctgcgcc agatcgttcc cgatcccacc
     1501 ggcgagcagc ccggcacgat cgtggtcgat acgaccggcc acttcctcta cctcgtgcgc
     1561 gaaggcggcc aggcgatccg atacggcgtc ggccttggac gcgcgggctt cgaatggtcg
     1621 ggcgacgcgg tcgtgcgctg gaagcagaaa tggccgaaat ggacgccgcc ggacgaaatg
     1681 atcgcgcgcc agcccgaact taccaaatat agcgccgata atggcggcat gcccggcggc
     1741 ctcaagaatc cgcttggcgc cagggcgctc tatctgttcc agggcgacgt cgacacgctc
     1801 tatcggttgc acggctcgcc cgaatggaat tccatcggca agtcggtttc ttccggctgc
     1861 gtgcgcctga tgaaccagga catcatcgac ctctacgacc gcgtgcccaa caagtcgaag
     1921 gtggtggtga cgagcaatgt cggccggccg atggcggcga acgcaagcag cggccaggct
     1981 attcccatcg atgacggcgt gccggaaggc tccgtccttc tcggccgcgt caagtcgttt
     2041 tcaaactcga tcttctgatt atccggctga ttgcctgacc agggacgccg gcatcgtcag
     2101 cgtcccttcg acaggctgcc cagaatgccg cgcacgatgg cgcggccaac ggaagaaccg
     2161 accgagcgca ccaccgactt gatcgccgct tcggcaacgg tctggcggtt ggaggcgcgc
     2221 gccggcgggc gtcggcccgt ttgcgccggc gcgttggcgt catcgccgcc gaagcccggt
     2281 atcgtccagc gagacccgcc ctcgcctgct ccctgctcgg catcctgcgc ggccttggtt
     2341 ttcttttgaa gtatttcgaa agccgattcg cgatcgaccg cctcgtcata ttgtcccgcc
     2401 accgggcttt cgaggatgat cttgcgccgt tcttccggcg ttatcgggcc gagccgcgag
     2461 gatggcgggc gaatcagcgt gcgctgcacc atggaaggaa tgcccttggc ttccagcgtc
     2521 gagaccagcg cctcgcccgt cgccagctgc gtgattaccg tggcgcaatc gaaatccgga
     2581 tttggccgga aggtgtcagc cgcggtcttc acggcatttt gctcgcgcgg cgtataggcg
     2641 cgcagcgcgt gctgcacgcg attccccagc tgcgccaaca ccatttcggg tacgtcgagc
     2701 gggttttgcg ttacgaaata aatgccgacg cccttggagc ggatgagacg cacgacctgc
     2761 tccacgcggt tgagcaacac cttgggggca tcgtcaaaaa gcaggtgcgc ttcatcgaag
     2821 aagaacacca gccttggctt ttcgggatcg cctatctccg gaagttcctc gaaaagctcc
     2881 gacatcagcc agagcaggaa ggtggcgtaa agccggggat tcatcatcag cttgtcggcg
     2941 gccaacacgt tgatggcgcc gcgcccgtcg cgcgtcgtgc gcatgatgtc ggcgatcttc
     3001 agcgccggtt cgccgaagaa atgcgccgcg ccctgctgtt ccagcaccag aagcgttcgc
     3061 tggatagcgc cgaccgacgg cttggtgaca ttgccgtaac gcgcgccgat ctccacggcg
     3121 cggctggcca tgttggcaag cagcgcctgc aaatccttca tgtcgagcag caaaagccct
     3181 tcgtcgtcgg cgatcttgaa ggcaatattc aaaacgcctt cctgcgcctc ggaaaggtcc
     3241 atcaggcggg acaggagcaa cggtcccatt tcggacacgg tggcgcggat cgggtggccc
     3301 tgctcgccga acagatccca gaagatcacc ggaaaatcct ggaactcgta cggatcgagc
     3361 ttgacctgct cggcgcgctt gacgaggaaa tcctggggcg tgcccttcat ggaaatgccc
     3421 gacaggtcgc ccttgatatc ggcgcagaac accggcacgc cggcattgga aaagctttcc
     3481 gcgagaattt gcaacgtcac cgtcttgccg gtgccggtcg cgccggtgac caatccatgg
     3541 cggttgccat attgcagcag aagctcttcg gcgcgctgat agctgtcgtc cggcttgcgg
     3601 ctggccccaa ggaagatgct tttgtcgtca gccatggttt tgcgtctccg aggttgcgat
     3661 gcttcgagcc gacagggcaa acgccccgca aggccaaagg tataaaggcg ctgccgcata
     3721 gcgacaatgg ccgccgccca aattgcgccg cgccttcggc ggcaggcgcc cttcatcggg
     3781 aaatcagccg gcaagcgacc ccttgtgcat tgcggtacga gattatcgcc cctacactga
     3841 caacaaggcc gatgcggcca agcgcgacga caagaaccgg gtgccgagac gttgatcagg
     3901 gacagtgaat ttccagctgc caaccggcgg caatgggttg ctttggccga gaaagcgctt
     3961 ggcgacacgg cgtttgaaga cgcactggtt tcgcgaagcg acgacgccat tcctatcgat
     4021 ccgctgcatg agcgcgcaat cgacgccgcc gccagcctgc gcgtcaggcc cgatctaccc
     4081 tggacgatca gccagcgcgt ggacgacccc gatgtcgagc gcgcgaaggc gcagatcgag
     4141 gaagacctgg cgcagggcgc gacggggctg tcgctggtct tcgagggcgc gccaaatgcc
     4201 ttcggctatg gcctgcccgc caccgcgcag gcgttggaat ccgttctcga aggcgtgccg
     4261 ctcaaccgca tccacctgcg aatcgatgtg catccgtcga gccgggccat ggccgactgg
     4321 ctggtcgggc tgctgacgcg ccgccgcgcc gatcctgaaa aactcaacgt atcgttcggc
     4381 atcgatccgg cagcgacatt tgccggaaca ggccggctgc gcatgtcgat cgaagccctg
     4441 caagcatcga tgccgcaatc gctggcgcat ttcttcgcca tgggcgtgcc cggcgtcctg
     4501 cttgaggccg acgggcgcgt cttccacaat tccggcgcga ccgaagcgca ggaactcggc
     4561 atcatgctgg cgtccgccgt gtcgcatctg cgcctgttcg aagaggcgcg ccaggccttg
     4621 atttacgccg cgccgcatat cggctttgcg ctcagcgtcg accagaacca gttcctgtcg
     4681 ctggccaagg tgcgggcgtt gcgggccttg tgggcacgca tccagcagac atgttccatc
     4741 cctgcttccg acgccaccat ccatgccgaa acctcgcttc gcatgatgac cgctctggac
     4801 cccgagacga atatcctgcg caataccatt gcctgtttcg cggctgccgc cggcggggcg
     4861 gattcgatct ccatcctgcc gcacaccatc acgcatgggc tgcctgcggg atttgcgcgc
     4921 cgcatcgcgc gcaacacgca gctgataatg gcgcgtgaaa gccatatcga ccatgtcaac
     4981 gatccggcgc gcggatcggg cggcgtcgag gcgctgacgg ctggcctttg cgaagccgca
     5041 tgggcggaat tccagcgcat cgaggcggag ggtggcgtgc tcgccagcct gcgtgaaggc
     5101 catatccaga agcgggtggc cgcggcgcgg ctggagcggc agaagctcta cctggccaat
     5161 aaacgcacca ttatcggcac gacgctttat cccgccaaga aggaacgccc ggtggagacg
     5221 ctcgccgcct tgaaacatgc cgggccgacc gacggtgtgg tgttttgcga accgatcctg
     5281 cctgcccggc tcgatcaatt tgcggaggcg gaccaatgat ccccgatttc agcaagatcg
     5341 cctggtccaa ggctcccgcc ctgcctcgcg atgttgcggg cgaatggctg acgccggaag
     5401 gcatcgccat caagggcctt tacagcgagg ccgatatcgc cagcctcccg gacctcgaca
     5461 cctatcccgg catcgccccc ttcatgcgcg gaccttatcc gaccatgtat gtgcagaagc
     5521 catggaccat ccgccaatat gccggctttt cgacggcgga agaatccaac gccttttacc
     5581 ggcgcaatct agccgccggc cagaagggcc tgtcgatcgc cttcgacctc gccacccatc
     5641 gcggctatga cagcgaccat ccgcgcgtgg ccggcgatgt cggcatggcg ggcgtcgcca
     5701 tcgattccat tctcgacatg cgccagctgt tcgatggcat cccgctcgac cagatgaccg
     5761 tttcgatgac catgaacggc gcggtgctgc ccatcctggc gctctacatc gttgctgccg
     5821 aagaacaggg cgtcgcccag aaagacctca ctggcaccat tcagaacgac attctgaagg
     5881 agttcatggt gcgcaacacc tacatctatc cgccaaaacc ttcgatgcgg atcgtttccg
     5941 acatcttttc ctatacgtcg cggcatatgc cgaagttcaa ttcgatctcg atttccggct
     6001 accacatgca ggaagcgggc gcgacggcgg acctggaact cgcctacacc atcgccgacg
     6061 gcatcgaata tgcgcgcgcc ggcgtcgccg ccgggctcga tatcgacaag ttcgcgccac
     6121 ggctttcctt cttctgggcc atcggcatga acttcttcat ggaagtggcc aagctgcggg
     6181 cggcgcgcct gatctgggcg caactgatgc agaagaattt cgcgccgaaa gatcagcgct
     6241 cgctgtcgct gcgaacccat tgccagacat cgggctggtc gctgacggcg caggacccct
     6301 acaacaacat cgcgcgcacg atgatcgagg ccatggccgc cacgcagggc cacacgcagt
     6361 cgctgcacac caattccttc gacgaagcgc tggcgctgcc gaccgaccat tcggcgcgta
     6421 tcgcccgcaa cacgcagctt gtcctgcaaa aagaatccgg caccacggcc atcatcgacc
     6481 catggggcgg ctcggcctat gtcgagcggc tgacgcatga cctggctgag cgcgcgctgg
     6541 cgcatatcga agaagtcgag cgcctcggcg gcatggcggc ggcaaccgaa aagggcatcc
     6601 ccaagctgcg cattgaggag gcgtcggcgc gcacgcaggc gcgcatcgat tccggcgacc
     6661 agattctcgt cggcgtgaac gcctatcgcc cggatagcga cacggatgtc gacgtgctga
     6721 agatcgacaa tgccgaggtg cgcgcgcgcc agctttcaaa gctgcaacaa ttgaagggca
     6781 cgcgtgacgt gggtgccgtg gaaagcgcgc tcgatgcgct gacgctggcg gcgaaaagcg
     6841 gcgaaaacct gcttgaattt tccattcgcg ccgcgcgcgc caatgccacc gtcggcgaaa
     6901 tctcgctggc gctggagaag gcgttcggcc ggcatgtggc ttcggtgcag acgatttccg
     6961 gcgtctaccg caaagcgctt ggcgagacgc ccgctgtcgg caggcttgag gaaaagcttg
     7021 cggcgttcga gaagaaggca ggcagcaagc cgcgcattct cgtcgccaag atgggtcagg
     7081 atgggcatga tcgcggtcag aaagtcatcg ccacggcctt tgccgacctt ggcttcgacg
     7141 tgacggtagg cgccatgttc cagacgcctg aggaaatcgc aaaactcgcc gtggaacata
     7201 atgtccacat cgtcggcgca tcgtcgctgg cggcgggaca tttgacgctg atccccgaac
     7261 tgaaggacgc gctggagaaa tccggccatg gcgacattct ggtcgtcgcc ggcggcgtca
     7321 tcccgccaca ggattacgac gccgttctaa aggcgggggc cgccgaaatc ttcccgcccg
     7381 gcactgtcat ccccgaagcg gccgagcgat tgatcgacag gctgctcgcc gccacctgac
     7441 ctgttataat cgatgttgca acacgtcgcg catgcgttat aatgcgtgtt gcaacagagg
     7501 tatcagccat gaacacgacc gttcgcaaga tcggaaattc ggaaggcgtg atcctgccga
     7561 aagaattgct cgaccgcctg aacatcaagg cgggcgacga actggaaatc atcgcgaccg
     7621 acaaaggcgt tactctgaag ccggtcgacg atagcttcga acggcagatg aaagccgcgc
     7681 gcgcgatgat ggacaaatac aaggtcgctc tgcaaaagct cgccgaatga gctgggaatt
     7741 tctgtcacgc cgcgtcgtgg aggcgatgca tgccgaacaa ctgcgcaggc atggcggagc
     7801 tcagggaaca cgggacgaga acgcccttga atcggcattg gcccgcccgc aaaataaagc
     7861 tgaatatggc gagcctacgg tccatgaact tacggcggca tatgtcttcg gcatcgcaag
     7921 aaatcatgct ttcgtcgatg gcaacaagcg caccgccatt gtggcggcgg gtacgttcct
     7981 gatcgtcaac ggccacacgc ttaccgccaa cgacggcact ttatatgagt tcgtgatggg
     8041 tgttgccgcg ggcacgatcg acgaggcggg cgccgccgcg ttcttccgcg accataccgt
     8101 caagatcgag ggctagagcg gttccgattt tgctgaatcg acaaaaccgc tctatctctt
     8161 tgttttggcc gcatttccgg acgcaaaacc gtttcacact tttgctggaa atgctctagg
     8221 caggccggtt tcagcctgcc agccccacga tctcccattc cttgccattg acggcagcaa
     8281 tgtcgccgat tttcttgcca aacagtgcgc gcgccatcgg cgagacatgc gagatggacc
     8341 ccttggcggg atcggcctcg tcctcgccga cgatgcgcca tgacacctcg ctgccatcct
     8401 cgctttccag cgccacggtc atgccgaaac gaaccacgcc gctgccggcc tcggccaccg
     8461 caagctccgc gctttcgcgc cgcgccatcc agtagcgcag gtcgcgcgaa accatggcga
     8521 tgcgctgctt gtccgccccg ctttcggcat ttttcaattc cacgcgcagc gccgcaagct
     8581 cggcctcgat cattgccaga ccgtgctgcg tcaccagatt gcgatgctgg ctgaccggcc
     8641 gctcgccaat gccggcgatt gcattttcgc tatcttcttc gcgggtgaaa gctctgctca
     8701 ttcttcgaac ctagagcatt tccagcaaaa gtgtgaaacg gttttgcgtc cggaaatgcg
     8761 gccaaaacaa agagatagag cggctttacc gattcggtaa aaatcagaac cgctctaggc
     8821 atggtcggcg ggcttcacaa gccatattgg ccggctggcc tcgcaccccg caccagcgcc
     8881 cggcggcgga atcaaccaat caagctcagg tcacgccagc gccgcggtcg gtcgcaggct
     8941 tgcgaccgaa aggccattcc aattcttcag caccgggcag gcctgggtga tcaaggtcgc
     9001 ggaaagcgct tcgtcgcttg caagcagctt tgccagaacg gccgcgatca tcgtttccgc
     9061 tccccggatt gcgccatcgt cgcatttcag cgcgatgccc agccccagtt ccggtatggc
     9121 cgcccaataa acgccttctg cccccgattt gacgaagatg cgtccaggag ccgcgcccat
     9181 caacgccgca tcggccttat ccgtgcccga aatcaggaat ggctcggcca tgcaggcgct
     9241 catcaggcgc ccggcggctt tcgcccgttc cggcggcagc ccattaccgg tgaccattcg
     9301 cgcaaagccg cgagcgaggt cgcgcagcgg caccgcatag gtcgggatcg agcaaccgtc
     9361 gatgccacga ttggcggcat tatgagccgc gccggttacg tcctccattg ccgcgcgaac
     9421 catttcttga aacgcatggc cggcctcgac gtagccgtga tgatcaatgc cagcgtgaac
     9481 gcaggtgcat agaaaaccgg aatgcttgcc cgagcaatta ttgtgcagcg cgcttggcgc
     9541 gccgccgccc tgcgccaacg ccacggtcgc agcctggctg gacggccag
//
LOCUS       DASYCS010000016         9871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3916867,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000016 DASYCS010000000
VERSION     DASYCS010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 9871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9871
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3916867"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1074)
                     /locus_tag="VGM46_00630"
     CDS             complement(<1..1074)
                     /locus_tag="VGM46_00630"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY4191127.1"
                     /translation="MISAGLPPKSVPGELFRADTGIFAWFSAPWKDEPQAVRAKLLAS
                     TFDRKFAVVFGSASVLILAGSALVFTHAWWPWAWIAADLILLVIRFHLMVECERGRRV
                     AAHSPLAQLMAAGALWSVTFGLGCYICAASGHMALTVLAGLNVAGVLGVATSRNAATP
                     RYAIFVMLAVSFPYVVGALLSREPGMAVVGIQMPVYIVGIVIVMLRNHAIAVRMIRAE
                     IDNRELAVKEALTGLPNRIFLQEKLRAMCEELAAPAANGGRPFAVLSMDLDGFKQVND
                     SFGHGVGDNLLRKVAKILTQTFREQDTICRIGGDEFVILLPNTSEIEATYLAKRSIDD
                     ISEPFDLGVATSVRIGLSIGSAFA"
     gene            complement(1171..1533)
                     /locus_tag="VGM46_00635"
     CDS             complement(1171..1533)
                     /locus_tag="VGM46_00635"
                     /inference="COORDINATES: protein motif:HMM:NF017470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HEY4191128.1"
                     /translation="MSLQQTLWAFFSFSGRLSRPAFALAGLLLYLIRFYPIYRIYNAE
                     GDEAAMTRWAAILLVMLCVLVLSHIALSVKRLHDFNRTGWYSLLFLIGDFLAFLILCL
                     PQGTAGPNRYGRQTDQPA"
     gene            complement(1544..2398)
                     /gene="dapD"
                     /locus_tag="VGM46_00640"
     CDS             complement(1544..2398)
                     /gene="dapD"
                     /locus_tag="VGM46_00640"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEY4191129.1"
                     /translation="MSKPDLASLEKTIDKAFEERDGISTATRGEVREAIEAALDLLDK
                     GNARVAEHQGDGKWHVNQWLKKAVLLSFRLAPMDLIAGAPGGAAWWDKVPSKFDGWGA
                     VDFEKAGFRAVPSSVVRRSAYVAPGVVLMPSFVNVGAYVDTGTMVDTWASVGSCAQIG
                     KNVHLSGGVGIGGVLEPMQAGPTIIEDNCFIGARSEVVEGCIVREGSVLGMGVFIGQS
                     TKIVDRATGEIFYGEVPANSVVVAGTMPGKPLPDGTPGPSLYCAVIVKRVDARTRSKT
                     SINELLRD"
     gene            2546..3385
                     /locus_tag="VGM46_00645"
     CDS             2546..3385
                     /locus_tag="VGM46_00645"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018426285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HEY4191130.1"
                     /translation="MNTEEKAGWTPLPHSDEDLERAKSVPDTPQTRSPTYRLAWNDED
                     FMTRRELRAVRLQLELLKPEMILAQRGIRSTVILFGGARIPEPGGEAWAAKNETQKKN
                     LEENSKYYEEARKFARLCSQQSASSAYREFVVVTGGGPGVMEAGNRGADDVGAPSIGL
                     NIVLPHEQAPNIYVTPELSFNFHYFAIRKMHFVMRAKAVAVFPGGFGTLDEFFETLTL
                     IQTGRMERVPIILFGKSFWSRALDLDFLAEQGTITPGDQDIIDYVDTAEEAWNIISRF
                     YKL"
     gene            complement(3429..4133)
                     /locus_tag="VGM46_00650"
     CDS             complement(3429..4133)
                     /locus_tag="VGM46_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015314425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HEY4191131.1"
                     /translation="MTELPDPARFSHVTDWVFDLDNTLYPHHSNLFSQIDVRMTAYVS
                     ELLTLPRDEARTLQKELYLKYGTTLNGLMELHGIDPDDFLEKVHDIDYSWLVPDPVLG
                     TAIRQLPGRKFIFTNGDRRHAERAARQLGILDHFDAIFDIVAAGLTPKPARQTYERFA
                     ELHAVTGANTVMFEDLARNLSVPKALGMTTVLVVPRNFEPTFSEIWERDPLHDDDVDF
                     VTDDLSGFLTTIAGAE"
     gene            4402..5568
                     /gene="argE"
                     /locus_tag="VGM46_00655"
     CDS             4402..5568
                     /gene="argE"
                     /locus_tag="VGM46_00655"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008777 - acetylornithine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HEY4191132.1"
                     /translation="MNTIEILDRLLGFPTVSRDSNLDLIGFVADLLERNGIASQIIHS
                     EDGHKANLFATVGPAGVPGVMLSGHSDVVPIDGQDWTLPPFAMTERDGKLYGRGAADM
                     KGFIAAALVAAIKASKMDLKTPLHLAISYDEEIGCIGVRSLVEMLRASPQKPLLCIIG
                     EPTNMQVATGHKGKIGARAFCRGREGHSALAPLALNAIHLGCDFIAALRREQDRLASD
                     GARDGDYDIPYTTVHVGKINAGVALNIVPNLCQVDFEIRNVAADNAEDVLDRLRAEAA
                     RIATEASAIAPEAAIDIEIFNTYPGLDTPAASQAVAFVKSLTGANGTIKVAFGTEGGL
                     FSRDVGTPTVVCGPGSMAQGHKPDEFVSIEQLGRCDEMLDKLLQRLTEATPLAV"
     gene            complement(5762..5835)
                     /locus_tag="VGM46_00660"
     tRNA            complement(5762..5835)
                     /locus_tag="VGM46_00660"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5801..5803),aa:Gly,seq:ccc)
     gene            5951..6214
                     /locus_tag="VGM46_00665"
     CDS             5951..6214
                     /locus_tag="VGM46_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006204546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6460 domain-containing protein"
                     /protein_id="HEY4191133.1"
                     /translation="MSALNRFLGDSPLRVLLKLIVVSFIVGLVMNAFGWSPLDVFYSV
                     QRFFLDIWHLGFRAFGNLASYLLLGAAVVVPAFILLRLLSYRK"
     gene            6345..7667
                     /locus_tag="VGM46_00670"
     CDS             6345..7667
                     /locus_tag="VGM46_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006204549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEY4191134.1"
                     /translation="MDLASKPFIVTNRSVLAIAVPMTLAYLTTPILGLVDTAVVGQFG
                     NAAMLGGLAAGALIFDVVFTSFNFLRSGTTGLVAQAFGRGDALEEQAIFWRAVLIAVV
                     VGVALAALAPLVSLGGQWFIDAAPGVNAAMDTYIRIRLLAAPFSLINYAILGYVLGRG
                     EGALGLLLQLVLNSINIVLCLFLGLKLGWGVAGVAWATVSGEFLAMLLGLAIVCRRFY
                     ALPRLSKDRILNRQAIMRMMALNRDIMIRSFSLLATFALFTRQGAQHGTVTLAANAVL
                     MNFFLVASFFLDGFATAAEQLAGRAVGARAQAAFRLAVRLTLLWGFALAGAATLVLLF
                     AGDRLIGLVTTAPDVRATAAAYLPWAAFTALSGVLAFEMDGVFIGATWSRDMRNMMLV
                     SFLVFVAALFVLTPFGNYGLWAALHIFLLTRGLSLLAILRLRLRGAFG"
     gene            complement(7664..8737)
                     /locus_tag="VGM46_00675"
     CDS             complement(7664..8737)
                     /locus_tag="VGM46_00675"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEY4191135.1"
                     /translation="MSMFDRIGQKLLFSLDPETAHGLSIAALRCGLPVSAAATHDRRL
                     ATSLCGIDFPNPLGMAAGYDKNAEVPDALLGLGFGFTEIGTITPLAQQGNPKPRIFRL
                     VEDKAVINRLGFNNEGHDAAERRLIARRGRTGIVGVNIGANKDSADRIGDYEKGIRRF
                     ASLAAYLTVNISSPNTPGLRNMQAREQLGELLGRVLAARTEASSRPPVFLKIAPDLAE
                     AELEDIAAEVTEKRIDGVIVSNTTLARGLKSSSAATEAGGLSGRPLFERSTIMLAKMR
                     KLLGPDAALIGVGGVDSAATALEKISAGADLVQLYTGMIYAGPALPARIVSGISDFVE
                     KQGLPSIRDLRDSNLYAWAAKPL"
     gene            complement(8750..9100)
                     /locus_tag="VGM46_00680"
     CDS             complement(8750..9100)
                     /locus_tag="VGM46_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019863164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HEY4191136.1"
                     /translation="MTRTIYKICPEVLWREAEKNGCFLGAPVDIADGFIHFSTAAQAG
                     ETARKHFSAQKDLLLIAVDGSALGDALRYEPSRGGDLFPHLYGALALDAVTCVRALPL
                     GADGLHEFPEMDGA"
     gene            9211..9651
                     /locus_tag="VGM46_00685"
     CDS             9211..9651
                     /locus_tag="VGM46_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015319249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191137.1"
                     /translation="MQLQHLIVSASFFSLFAMPALAEDAGRYRLEKSTDGYIRMDTQT
                     GAMSRCEDRAGQLVCRMAADERSAYQNEIDDLQDSVKALDARVGKLENSLSARLESKL
                     PSEEEFNKTMSYMERFMRGFMGIVKDMDKNGDNTAPAKPLPEKT"
     gene            complement(9735..>9871)
                     /gene="mscL"
                     /locus_tag="VGM46_00690"
     CDS             complement(9735..>9871)
                     /gene="mscL"
                     /locus_tag="VGM46_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912648.1"
                     /note="homopentamer; channel that opens in response to
                     pressure or hypoosmotic shock; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEY4191138.1"
                     /translation="IFLMVKAVNTMRRRVESEKPAEAAAPPPADVALLTEIRDLLARK
                     "
BASE COUNT         1817 a         3063 c         3149 g         1842 t
ORIGIN      
        1 cgcaaaggcg ctgccgatgc tcaggccgat ccggaccgaa gtggcgacgc cgaggtcgaa
       61 cggttccgag atatcgtcga tggagcgctt tgccagatag gtcgcctcga tttcggaggt
      121 gttgggcagc aggatgacga attcgtcgcc gccgatgcgg cagatggtgt cttgctcccg
      181 aaaagtctgc gtcaggattt tcgccacttt gcgcagcaga ttgtctccga cgccatggcc
      241 gaaactgtca ttgacctgtt tgaacccatc gaggtccatg ctgagcacgg cgaacggccg
      301 gcctccattg gcggcgggcg cggcaagttc ctcgcacatg gcgcggagct tttcctggag
      361 aaaaatacgg tttggaagcc cggtcagcgc ttccttgacg gccagttcgc ggttgtcgat
      421 ttcagcccgg atcatgcgga ccgcgatggc gtggttgcgc agcatgacga tgacgatgcc
      481 gacgatgtag accggcattt gaatgccgac cacggccatg cccggttctc gcgacaaaag
      541 cgcgccgacg acatagggaa agctcaccgc cagcatgacg aagatggcgt agcgaggcgt
      601 tgcggcgttg cgcgatgtcg ccacgcccag aacgccggcc acgttcagtc cggcaagcac
      661 ggtcagcgcc atatggccgc tggcggcgca tatgtagcac cccaagccga aggttaccga
      721 ccacagcgcg ccggcagcca tgagctgggc aagcggagag tgagcggcga cgcgccgtcc
      781 ccgttcgcat tcgaccatca ggtggaaacg gatcacgagc aagatgagat cggccgcgat
      841 ccatgcccat ggccaccagg catgggtaaa gaccagggcg cttccagcca ggatcaaaac
      901 gcttgccgaa ccgaacacga cagcgaattt gcggtcgaat gtcgaggcaa gcagcttggc
      961 ccgaaccgct tgcggctcgt ccttccaagg cgctgaaaac cacgcaaaaa taccggtatc
     1021 tgctctgaaa agctcacccg gcactgattt tggcggcaat ccggcggata tcatccatct
     1081 ctccgtgtcg agggacacag ctatccccga cacggtgcgg caaggtgatt aagatttatt
     1141 tagggtttgg cgcccgcgcg gccgacaggc ctatgcgggc tggtcggtct ggcggccgta
     1201 gcggttgggg ccggcggtgc cttgcggcag gcacaggatc aggaaggcca ggaaatcgcc
     1261 gatgaggaac agcaggctgt accagccggt ccggttgaaa tcatgcagcc ttttgaccga
     1321 cagcgcgata tgcgacagga ccagcacgca cagcatcacc agcaatatgg cggcccaacg
     1381 ggtcatcgcg gcctcgtcgc cctcggcgtt atatatcctg tagattggat agaaacggat
     1441 caggtaaagc agcagtccgg ccagcgcgaa ggccggccgg ctcagccggc ccgaaaaact
     1501 gaaaaaggcc cacagggttt gttgaaggct cacgctcgcg gccttagtcg cgcagcaatt
     1561 cgttgatcga cgtcttcgag cgcgtcctgg cgtcgacccg cttgacgatg acggcgcagt
     1621 ataggctcgg tccgggggtg ccgtccggca agggcttgcc gggcatggtg ccggccacca
     1681 ccaccgaatt ggctggaact tcgccgtaga atatctcgcc ggtggcgcgg tcgacgatct
     1741 tggtcgactg gccgatgaag acacccatgc cgagcacgga accttcacgc acgatgcagc
     1801 cctcgacgac ctcggagcgc gcgccgatga agcaattatc ctcgatgatg gtcgggcctg
     1861 cctgcatcgg ctccagcacg ccgccgatgc cgacgccgcc cgaaagatgc acgttcttgc
     1921 cgatctgcgc gcacgagccg acagacgccc aggtgtcgac catcgtgccg gtatcgacat
     1981 aggcgccgac attgacgaag gacggcatca ggacgacgcc cggcgctaca taggccgagc
     2041 ggcgcacgac cgaggaaggc acggcacgga agccggcctt ctcgaagtcc accgcacccc
     2101 agccgtcgaa tttcgaaggc accttgtccc accacgccgc cccgccgggg gcgccggcga
     2161 tgaggtccat cggcgcaagg cggaaggaga ggagcacggc cttcttcagc cactggttga
     2221 catgccattt gccgtcgccc tggtgctcgg cgacgcgggc attgcccttg tcgagcaggt
     2281 cgagcgcggc ctcgatggcc tcgcggactt cgccgcgtgt cgccgtggaa atgccgtccc
     2341 gctcctcgaa tgccttgtcg atggtctttt cgaggctggc cagatcgggc ttagacatgc
     2401 aatggctcca tttccacgct gttaagggct ggcccttaac ctgttatcaa aatcgcgcgg
     2461 accctatgtt aaggtccggg acgggtcaat cgcgcccgcg ccaagggacg gcgcagctac
     2521 gcagcatttt tggggatact ctggcatgaa taccgaggaa aaggcgggct ggacgccgct
     2581 tccgcattcg gacgaggatc tggagcgggc aaaaagcgtg cccgacacgc cgcagacgcg
     2641 ctcgccgacc tatcgcctgg cctggaacga cgaagacttt atgacgcggc gcgaactgcg
     2701 cgccgtgcgc ctccagctcg aattgctgaa gccggaaatg atcctggccc aacgtggcat
     2761 tcgctcgacg gtgatcctgt tcggcggcgc gcgaattcct gaacccggtg gtgaagcgtg
     2821 ggcggcgaag aacgagacgc agaaaaagaa cctcgaggaa aacagcaaat attatgagga
     2881 ggcacgcaag ttcgcccgcc tctgctcgca gcagtcggca tcgtcggcct atcgtgagtt
     2941 cgtcgtggtg accggcggcg gtccgggcgt gatggaggcc ggaaatcgcg gtgccgacga
     3001 tgtcggcgcg ccgtcgatcg gcctcaacat cgtcctgccg cacgagcagg cccccaacat
     3061 ctacgtgacg ccggagctga gcttcaactt ccactatttc gctatccgca agatgcattt
     3121 cgtcatgcgc gccaaggcgg tggcggtgtt tcccggcggc ttcggcaccc tggacgagtt
     3181 tttcgagacg ctgacgctga tccagaccgg gcgcatggag cgcgtgccga taatcctgtt
     3241 tggcaaatcg ttctggagcc gcgcgctcga tctcgatttc ctggccgaac aaggcacgat
     3301 aacgccgggc gaccaggaca tcatcgacta tgtcgataca gccgaggaag cctggaacat
     3361 catcagccga ttctacaagc tgtgagggga gtaggggagt aggggagtag gttcgacctc
     3421 tcccgttgct actcggcgcc ggctatcgtg gtcaggaacc ccgacaggtc gtcggtgacg
     3481 aaatccacgt catcgtcgtg cagcgggtcg cgctcccata tctcggagaa ggtcggctcg
     3541 aaattgcgcg gcaccaccag caccgtggtc atgccgagcg ccttcggcac ggaaagattg
     3601 cgcgccagat cctcgaacat caccgtattg gcgccggtga cggcgtgaag ctcggcaaag
     3661 cgctcatagg tctggcgcgc cggcttaggc gtcagcccgg cggcgacgat atcgaagatg
     3721 gcgtcgaaat gatcgaggat gccgagttgc cgcgcggcgc gttcggcatg cctgcgatcg
     3781 ccattggtga agatgaattt ccggcccgga agctggcgga tggcggtgcc gagaaccggg
     3841 tccggcacca gccaggaata atcgatgtcg tggacctttt cgaggaaatc gtccgggtcg
     3901 atgccgtgca actccatcag cccgttcagc gtcgtgccgt atttcaggta gagttccttt
     3961 tgcagcgtcc gcgcctcgtc gcgcggcagc gtcagcaact ccgacacata ggccgtcatc
     4021 cgcacgtcga tctgcgagaa caggttcgag tgatgcggat agagcgtgtt gtcgaggtcg
     4081 aacacccagt cggtgacatg ggagaagcgg gcgggatcgg gaagctcggt catgcggccc
     4141 ttatggcacg gaatggcgtt cgggaaaccg cctgaccggc aatttttagc gggccagcgg
     4201 caattcgaac ccgattgccg cagatgacgc gggggcaggc gtcaaaggaa ttgcgccgtc
     4261 ccggcccgcc ggctcggcat ggcccctggc ccgataaggt tcgtcgaatt ccgccagctt
     4321 cggattgccc tgaacggacg ggcttcaaag cggctgcgat atggtgttat caagcccata
     4381 catcatgagg cggatttggt aatgaacacc attgaaatac tggacaggct gctcggcttt
     4441 ccgaccgtga gccgcgattc caatctcgac ctgatcggtt tcgtggccga tcttctggag
     4501 cgcaacggca tcgcctcgca gatcatccac agcgaggatg gccacaaggc gaacctgttc
     4561 gccacggtcg gtccggcggg tgttcccggc gtcatgctgt ccggccacag cgatgtcgtg
     4621 ccgatcgacg ggcaggactg gacattgccg cccttcgcca tgacggagcg cgacggcaag
     4681 ctttacgggc gtggcgccgc cgacatgaag ggcttcatcg ccgccgctct ggtcgccgcc
     4741 atcaaggcgt cgaagatgga cctgaagacg ccgctgcatc ttgcgatttc ctatgacgag
     4801 gaaatcggct gcatcggcgt acgcagcctg gtcgaaatgc tgcgggcatc gccgcaaaag
     4861 ccgctgctgt gcatcatcgg cgaaccgacc aacatgcagg tggcgacggg ccacaagggc
     4921 aagatcggcg cgcgcgcgtt ctgccggggg cgcgagggcc attcggcgct ggcgccgctg
     4981 gcgctcaacg ctatccacct cggctgcgat ttcattgccg ccctgcgccg cgaacaggac
     5041 aggctggcaa gcgacggcgc gcgcgacggc gactacgaca ttccctacac cacggtccat
     5101 gtcggcaaga tcaatgccgg cgtggcgctg aacatcgttc cgaacctgtg ccaggtcgat
     5161 tttgaaatcc gcaacgtcgc ggcggacaat gccgaggacg tgctggacag gctgcgggcc
     5221 gaggcggcgc gcatcgcaac cgaggcgtcc gcaatcgcgc cggaagcggc catcgatatc
     5281 gagatattca acacctatcc gggtctcgac acgccggctg cctcgcaggc cgtcgctttc
     5341 gtcaagtcgc tgacgggggc caatggcacc atcaaggttg ccttcggcac cgaaggcggc
     5401 ctgttcagcc gcgatgtcgg cacgccgacg gtggtctgcg gtccgggttc gatggctcaa
     5461 ggccacaagc cggatgaatt cgtcagcatc gagcaactgg gccggtgcga cgagatgctg
     5521 gacaagctgc tgcaacgcct gaccgaggca acaccgctgg ctgtgtaaat taaagcgccg
     5581 ccctgtcggc gtcgaagacg ccttgcacca ctgcgaagcg ccggcaatgg tcggtaaagc
     5641 cctgcacggt ttgcgtccgg tgccgggcat ccagaaccgc cgagggtaag gggggcgagg
     5701 tcgccgccag cggagcgcgc gccgccgggc ggcggataaa tcgatcggaa cggaaggaaa
     5761 ttggagcggg tagcgggaat cgaacccgcg cattcagctt gggaagctag caggctacca
     5821 ttacatcata cccgcgctgc cagcctgtta tcatgaagcc gcaccgcgag gcaatcgcaa
     5881 agccgcaggc ttgaagcctt tgcggcaaat gcgtatcagg tttacagccg aacgattctg
     5941 gagacgcgcc ttgtccgcat tgaaccgctt tctcggcgac agcccgcttc gggtgctgct
     6001 caagctgatc gtcgtctcgt ttatcgtcgg cctcgtcatg aacgccttcg gctggtcgcc
     6061 gctcgacgtg ttctacagcg tccagcgctt cttcctcgac atctggcacc tgggtttccg
     6121 cgctttcggc aatcttgcca gctatctgct gcttggggct gcggtcgtgg tgccggcctt
     6181 catcctgctg cgcctgctca gctaccggaa atagaccggc gggcgcgagt ccgtacttgg
     6241 gctgctccag tagccgaatt ccgttatccg cgcaggccgt gctgttgcgc ttaaagccga
     6301 tccaaggcac atcgatcacc gctcctgagt gaggatagcc gcccttggac ctggcttcta
     6361 agcccttcat agtcaccaat cgctcggtcc tggccatcgc ggtgccgatg acgcttgcct
     6421 atctgacgac gccgatcctc gggctggtcg atacggcagt ggtcggccag ttcggaaatg
     6481 ccgcgatgct cggcggcctc gctgccggcg cgctgatttt cgacgtggtt ttcaccagct
     6541 tcaatttcct gcgttcgggc actacagggc tggtcgcgca ggcgttcggg cgcggcgacg
     6601 cgctggaaga gcaggccatc ttctggcgcg ccgtgctgat tgccgtcgtc gtcggtgttg
     6661 cgctggcggc gttggcgccg ctggtctcgc tcggtggcca atggttcatc gacgccgcac
     6721 ccggcgtcaa tgcggcgatg gatacctaca tccgcatccg cctgctcgcc gccccgttct
     6781 cgctcatcaa ctatgcgatc ctcggctatg tgctgggccg tggcgaaggc gctctcggcc
     6841 tgcttttgca actggtgctg aacagcatca acatcgtgct ttgcctgttc cttggcctca
     6901 agctcggctg gggcgtggca ggcgtcgcct gggcaaccgt cagcggcgag tttctggcga
     6961 tgctactcgg cctggcgatc gtttgccggc gtttctacgc gctgccgcgc ctgtcgaagg
     7021 accgtatcct caatcgccaa gccatcatgc gcatgatggc gctcaaccgc gacatcatga
     7081 tccgctcgtt ttcgctgctg gcgactttcg cgctgttcac ccgccagggc gcgcagcacg
     7141 gcacggtgac gctggccgcc aacgcggtgc tgatgaattt cttcctggtc gccagcttct
     7201 ttctggatgg cttcgccacc gctgccgaac agctggcggg gcgcgcggtt ggcgcgcggg
     7261 cgcaggcggc ttttcggttg gcggtgcgcc ttaccttgtt gtggggattt gcgctggcgg
     7321 gcgctgccac gctcgtcctg ctcttcgccg gtgacaggct gatcggcctc gtcaccacgg
     7381 cgccggatgt ccgggccacc gctgccgcct acctgccctg ggccgccttc accgcgttga
     7441 gcggcgtgct ggctttcgag atggacggcg tcttcatcgg cgcaacatgg tcgcgcgaca
     7501 tgcgcaacat gatgctggtg tcgtttctgg tgttcgtggc ggcgctgttc gttttgacgc
     7561 ccttcggcaa ttatggcttg tgggcggcct tgcacatctt ccttttgacg cgcggcttaa
     7621 gcctgctggc aatattgcgg ctgcggttgc gcggcgcttt cggttagagc ggcttggccg
     7681 cccaggcgta aagattgctg tcgcgcaggt cgcggatcga cggcaggccc tgcttttcga
     7741 caaaatcgct gatgcccgaa acgatgcgcg ccggcagcgc cggcccggca tagatcatgc
     7801 cggtgtaaag ctgcacaaga tcggcgcccg cgctgatctt ctccagcgcc gtcgccgccg
     7861 aatcgacgcc gccgacgccg atgagggccg cgtcgggtcc aagcagtttg cgcatttttg
     7921 caagcatgat ggtcgagcgt tcgaacagcg gcctgcctga aagcccgccg gcctccgtgg
     7981 cggcgctcga acttttcagc ccccgtgcca gcgtggtgtt ggaaacgatg acgccgtcga
     8041 tccgcttttc cgtgacctcg gctgcgatat cctcaagttc ggcctcggcc agatccggtg
     8101 cgatcttgag gaagaccggc ggcctggaag aagcttcggt gcgcgccgcc aaaacacggc
     8161 ccaggagttc gccaagctgc tcgcgcgcct gcatgttgcg caggcccggc gtattgggcg
     8221 aggagatgtt gaccgtcagg taggcggcca gcgaggcgaa gcggcggatg cctttttcat
     8281 agtcgccgat gcggtcggcg ctgtccttgt tggcgccgat attgacgccg acgatgccgg
     8341 tgcggccacg gcgcgcgatg aggcggcgct ccgccgcatc atgtccttca ttgttgaagc
     8401 ccagccgatt gatgaccgcc ttgtcctcga ccagccggaa gatgcgcggc ttcggattgc
     8461 cttgctgcgc cagcggcgtg atggtgccga tctccgtgaa accgaaaccc agtccaagca
     8521 gcgcgtccgg cacttcggcg tttttatcgt agcccgccgc catgccgagc ggattaggaa
     8581 aatcgatgcc gcaaaggctc gtcgccagcc ttctgtcatg cgtcgccgcc gcgctcaccg
     8641 gcaggccgca gcgcaatgcc gcgatcgaca ggccatgggc ggtctccggg tcgagggaga
     8701 acagcaactt ctggccgatg cggtcgaaca tgctcattca ccgtccattt caggcaccgt
     8761 ccatttcagg aaattcgtgc aagccatccg cccccagcgg cagcgcgcgc acgcaggtca
     8821 cggcatcgag ggccagcgcg ccataaagat gcggaaacag atcgccgccg cgcgaaggct
     8881 cgtatcggag cgcgtcgccc agcgcgctgc catcgaccgc gatcagcagc aggtcttttt
     8941 gcgccgaaaa atgctttctc gccgtctcgc cagcctgcgc ggcggtcgag aaatgaatga
     9001 agccgtcggc aatgtcgacc ggtgctccaa ggaagcagcc gttcttttca gcttcgcgcc
     9061 acaaaacttc aggacagatc ttgtagatgg ttcgcgtcat cgctgcgcta tagtccgaat
     9121 aggcggcgcg gaaaagggcg gcgcgaagct cctccccatt tcaggcgcaa acatgcgata
     9181 atccttgtct gacgaccgat ggagaaaaag atgcagcttc aacatctgat tgtttccgcc
     9241 tcgttcttct cgcttttcgc catgcccgcg cttgccgagg acgccggacg ctaccggttg
     9301 gagaaatcca cggacggcta tatccgcatg gatacgcaga ccggagcgat gtcgcgatgc
     9361 gaagaccgcg ccggacagtt ggtctgccgt atggcggcgg acgagcgcag cgcctaccag
     9421 aacgagatcg acgatctcca ggacagcgtg aaggcgctcg atgcccgtgt cggcaagctt
     9481 gagaactcgc tttccgcccg cctggaatcg aagcttccaa gcgaagagga attcaacaag
     9541 acgatgagct acatggagcg cttcatgcgc ggcttcatgg gcatcgtcaa agacatggac
     9601 aagaatggcg acaataccgc cccggcaaag ccgctgcctg agaaaactta aacccttgaa
     9661 cccgattgcc ccggcagccg gatgcggctc cgggacaatc gcttataatg ggcgcctaca
     9721 ggcgtgtggc gcgcctattt cctggcgagc aggtcgcgga tttcggtcag cagcgcgaca
     9781 tcggcgggcg gcggggccgc agcctcggca ggcttttcgc tctcgacccg acgacgcatc
     9841 gtgttgacgg ctttcaccat caggaaaatg a
//
LOCUS       DASYCS010000017         6199 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1162265,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000017 DASYCS010000000
VERSION     DASYCS010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 6199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6199
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162265"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="VGM46_00695"
     CDS             <1..486
                     /locus_tag="VGM46_00695"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013894792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HEY4191139.1"
                     /translation="ALFGGGADNWTGRGTGVDDAGLNRKIAAIEAGLKRHAAILSDPL
                     AIAAALGGRELAAILGATLAARHHGIPVLLDGFVSSAAAAPLAKLHPEGLAHTLAAHV
                     SAEAGHRRLLEALSLPPLLDLGMRLGEGSGACLAVNIVRSALECHAHMASFAEAGVSE
                     K"
     gene            complement(506..1243)
                     /locus_tag="VGM46_00700"
     CDS             complement(506..1243)
                     /locus_tag="VGM46_00700"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY4191140.1"
                     /translation="MTRKDIEIKTNDGVAKAGLFKPANASSGNAGVILYMDAFGPRPA
                     LDGMAERLAGEGYVVLVPDLFYRSGAYGPLDAKTSFSVESTANQIRGMMAATTQAMTQ
                     ADTGAFLDALAKDGATGSVGTVGYCMGGARALNGAAAHADRIKAAASFHGGNLASDAP
                     DSPHRHAAQIKAKVYVGSSGVDKSFPPEQSARLAEALREAEVDHVLENYVGMAHGWCV
                     PDHDVYDETGAERHWKRLITFFGESLR"
     gene            complement(1338..2024)
                     /locus_tag="VGM46_00705"
     CDS             complement(1338..2024)
                     /locus_tag="VGM46_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013894986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191141.1"
                     /translation="MSATQDILPELSAVLAENGLILRGGFNFDAGEDAPDGLSGARAQ
                     AVVLVGQAGASTWPYFSRWHESQPEDLADPLDTWSRQVTGAAAEKFAARAVGPNDKPY
                     LPFQQWAMRAERLKPSPLGILMHPRYGLWHAFRGALLFENEISFPAAETPVHPCDACV
                     DKPCLKSCPVDAYSPDGFAYRACLAHVRGSRGDACRVGGCLDRNACPHGVHYRYPQDM
                     QAFHMKAFAR"
     gene            complement(2130..3704)
                     /locus_tag="VGM46_00710"
     CDS             complement(2130..3704)
                     /locus_tag="VGM46_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019858696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEY4191142.1"
                     /translation="MSEQATVDQEASTARRGRAASGGAAARRAARSGGGPGTQLTYIK
                     RNIKVYDVLDEEGLALIEKNTDTVLEETGIIFRDDAEALQLWKEAGADVKGERVHFPK
                     GLCRSLLKTAPSVYTQHARNSERSVQIGGNATVFAPVYGPPFVRDLDGNRRYATIEDF
                     RNFVKLAYMAPSIHHSGGTVCEPVDVPVNKRHLDMIYAHIKYSDKPFMGSVTAPERAE
                     DTVAMAKIVFGDDFVEKNTVLTSLINANSPMVFDETMLGALKVYARHNQACIVTPFIL
                     AGAMSPVTVAGTLTQVLAEVLAGASFTQLVNPGAPVLFGTFASSISMQSGAPTFGTPE
                     PSLVSYGAGQLARRVGLPFRTGGSLCASKVEDAQAAYESANTLNSTILAGTNFVLHAA
                     GWLEGGLASSYDKFMMDIDQLGMTQKFAEGIDLSENGQAMDAIRQVGPGSHYLGCDHT
                     QANFQTAFYRSTIADNNSFEQWLAEGEKTAPQRANILARHWLESYEAPFLDPGIDDGL
                     KDFIARKKESMPDAFT"
     gene            3984..4682
                     /locus_tag="VGM46_00715"
     CDS             3984..4682
                     /locus_tag="VGM46_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HEY4191143.1"
                     /translation="MADDEIILSELSDDELVQQMHDDLYDGLKEEIEEGTNILLGRGW
                     VPYKVLTEALVEGMRIVGEDFRDGILFVPEVLLSANAMKAGMFILRPLLAATGAPKQG
                     KMVIGTVKGDIHDIGKNLVGMMMEGAGFDIIDLGINNSVEKYLAAIEEHQPDILGMSA
                     LLTTTMPYMKVVIDTMKEKGIRDDYVVMVGGAPLNEEFGKAVGADAYCRDAAVAVETA
                     KDFMKRKHNVRANA"
     gene            complement(4785..4940)
                     /locus_tag="VGM46_00720"
     CDS             complement(4785..4940)
                     /locus_tag="VGM46_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003530686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin"
                     /protein_id="HEY4191144.1"
                     /translation="MTLSRYASIAAIASLVLVTGCANTIRGAGQDTANTVNATQHAGK
                     KIANSTN"
     gene            5003..5647
                     /locus_tag="VGM46_00725"
     CDS             5003..5647
                     /locus_tag="VGM46_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HEY4191145.1"
                     /translation="MKRADEVAEAEKAARSGRLLVIACGMLAREILAVKQQLKLDHLD
                     LTCLPADFHFYPDRIAPAMDKAITEARAGGYDHIFVGYADCGTGGNLDHICEKHGVER
                     MAGPHCFAFYQRMEAYREVAEDDMMSFYMTDFLCRQFDAFFMKPLGLDKHPELIQDYF
                     GNYEKLIYLAQTDDPELDKVAEAAAKMLGLVYERRPTGYGDLPAGLARAAASVV"
     gene            5670..>6199
                     /locus_tag="VGM46_00730"
     CDS             5670..>6199
                     /locus_tag="VGM46_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4893 domain-containing protein"
                     /protein_id="HEY4191146.1"
                     /translation="MNLRKATLTACIALFAGSALADGVVQKLITPADKARLDHYVETR
                     KTALDEAKAGSPADVKALNALLARRLVSFSDKDLTGNWQCRTIKLGGDLGALVIYGWF
                     ECKVTDDGSGWLLEKTSGSQRTKGRFYDDGEKRSIYLGSFYVSGDPVKPYASGPGTDQ
                     VGYAFRTDANEFRIEFP"
BASE COUNT         1182 a         1925 c         1880 g         1212 t
ORIGIN      
        1 gccctgtttg gcggcggcgc ggacaactgg acaggacgcg gcaccggcgt ggacgatgcc
       61 ggcttgaacc ggaagattgc ggcaatcgag gccgggctga agcgccatgc cgcaatcctt
      121 tccgatccgc tggcgattgc ggcagctctt ggcggccgcg aactggctgc gattctaggc
      181 gcgacgctgg cggcccggca ccacggcatt cctgttctgc tcgatggctt cgtctcgtcc
      241 gccgccgccg cgcccctggc caagcttcac ccggaaggtc tcgctcacac gctggcggcc
      301 catgtttcgg cggaagcagg ccaccgccgc ctgcttgaag cactctcttt gccgcccttg
      361 ctcgatctcg gcatgcgcct tggcgaaggt tcaggcgcat gccttgccgt caacatcgtg
      421 cgctcggctc tggaatgcca tgcgcacatg gcaagctttg ccgaggccgg tgtctcggag
      481 aaataaggct aacgtttcga acagcttaac gaagcgactc gccaaagaag gtgatgaggc
      541 gcttccagtg gcgctcggcc ccggtctcgt catagacgtc atggtcgggc acgcaccagc
      601 catgcgccat gccgacatag ttctccagca catggtcgac ttccgcttcg cgcagcgcct
      661 ctgccaaccg cgccgattgc tcgggcggga agctcttatc gacgccggaa gagccgacat
      721 agaccttggc cttgatctgc gcggcatggc gatgcggact gtccggcgcg tcgctggcaa
      781 gattgccgcc gtgaaagctg gctgcggcct tgatgcggtc cgcatgcgcg gccgcgccgt
      841 tcaaggcccg cgcgccaccc atgcaataac caaccgtgcc gaccgaaccc gttgcgccgt
      901 ccttcgccaa ggcatcgagg aatgcgccgg tgtcggcctg cgtcatcgcc tgggtggtgg
      961 cggccatcat gccgcgaatc tggttcgccg tggattcaac ggaaaagctc gtcttggcat
     1021 cgagcggacc ataggcgccg ctgcggtaaa acaggtccgg caccaatacg acataaccct
     1081 cgccggccag tcgctccgcc attccatcca gcgccggccg agggccgaat gcgtccatat
     1141 acaggatcac gcccgcattg ccggacgagg cattggccgg cttgaaaagc ccggccttcg
     1201 ccacgccgtc attggtcttg atttcgatgt cttttctggt cacggcgaac tccttgatta
     1261 tggccgcgat agatatccgc cgaactggca agcgcaagcc atcgacgaca aaccgccatc
     1321 aacatttcgt gcggagacta ccgcgcgaag gctttcatat gaaaagcctg catatcctgc
     1381 ggatagcggt agtgcacgcc atggggacag gcgttgcggt ccaggcagcc gccgacgcgg
     1441 caggcatcgc cgcgcgaccc gcgcacatgc gcaagacacg cccgataggc aaagccatcc
     1501 ggcgaatagg catcgaccgg acaggatttc aggcatggct tgtccacgca tgcatcgcat
     1561 ggatggaccg gcgtttcggc agccggaaac gaaatctcgt tctcgaaaag caatgctccg
     1621 cgaaaagcgt gccaaagtcc gtatcgcgga tgcatgagaa tgccgagcgg cgacggcttc
     1681 agcctttccg cccgcatggc ccattgctgg aacggcagat agggcttgtc gttcgggcca
     1741 accgccctcg ccgcgaattt ttcggcggca gcgcctgtca cctgccgcga ccatgtatcg
     1801 agcgggtcgg ccaggtcttc cggctggctt tcgtgccatc gtgaaaaata aggccatgtc
     1861 gaagcgccgg cctggccgac aagcactacc gcttgggcgc gcgcgccgga caacccgtcc
     1921 ggcgcgtctt caccagcatc gaagttgaaa ccgccacgca atatcaggcc gttttcggcg
     1981 aggaccgccg acaattcagg caggatatct tgcgtcgcgc tcatggcttc gaggcaatac
     2041 tacttcttgt ccgaatcgga gaatgcgctc cgccagactt ccttatggat aatgtcggcc
     2101 actttagccc ggcctgcatc gggctccttt caagtgaagg cgtcgggcat ggactccttc
     2161 ttcctggcga tgaagtcctt caagccgtca tcgatgcccg gatcgaggaa cggcgcttcg
     2221 tagctttcca gccagtggcg cgccagaatg ttggcgcgtt gcggcgcggt tttctcgccc
     2281 tcggccagcc actgctcgaa cgaattattg tcggcgatgg tggaacggta gaaggcggtc
     2341 tggaaattcg cctgcgtgtg gtcgcagccg agatagtggc tgcccggccc gacctgccgg
     2401 atggcgtcca tcgcctggcc gttttcggaa agatcgatgc cttccgcgaa tttctgggtc
     2461 atgccgagct ggtcgatatc catcatgaac ttgtcgtagc tggaagccag cccgccttcg
     2521 agccagccgg cggcatgcag cacgaaattg gtcccggcaa gaatggtcga gttcagcgta
     2581 ttggcgcttt cataggctgc ctgcgcatcc tccactttcg aggcgcatag cgagccgccg
     2641 gtgcggaatg gcaggccgac gcgacgcgcc agctgccccg caccgtagga aaccagcgac
     2701 ggctccggcg tgccgaaggt cggcgctcca gattgcatgg agatggacga ggcgaaggtg
     2761 ccaaacagca ccggtgcacc cggattgacc aactgggtga aggaagcgcc ggccagcact
     2821 tccgccagca cctgtgtcag tgtcccggcc accgtcaccg ggctcatcgc gccggcaagg
     2881 atgaacggcg tgacgatgca ggcctggtta tggcgcgcat agactttcag cgcgcccagc
     2941 atcgtctcat cgaacaccat cggcgaattg gcgttgatca ggctggtcaa caccgtgttt
     3001 ttctcgacga agtcgtcgcc gaacacgatc tttgccatcg caaccgtgtc ttccgcgcgc
     3061 tcaggcgcgg taaccgaacc catgaacggc ttgtcggaat atttgatgtg cgcatagatc
     3121 atgtcgaggt ggcgcttgtt gaccggcacg tcgaccggct cgcaaaccgt gccgcccgaa
     3181 tggtggatcg acggcgccat ataggccaat ttcacgaagt tgcggaaatc ctcgatcgtg
     3241 gcgtagcggc ggttgccgtc gagatcgcgc acgaagggcg ggccgtagac cggcgcgaaa
     3301 accgtggcgt tgccgccgat ctgcacggaa cgctcgctgt tgcgggcatg ctgcgtatag
     3361 accgaggggg cggttttcag cagcgagcgg cacaggcctt tcgggaagtg caccctttcg
     3421 cccttgacgt cggcgccggc ttctttccag agttgcagcg cttctgcgtc atcgcgaaag
     3481 atgatgccgg tctcttcgag caccgtatcg gtgttcttct cgatcagcgc caagccttcc
     3541 tcgtccagta cgtcgtagac cttgatgttg cgcttgatat aggtgagctg ggtgccgggt
     3601 ccgccgccgg agcgcgccgc gcgccgtgcc gctgcgccgc cgctggctgc gcgaccgcgc
     3661 cgggcggttg acgcctcctg atccaccgtc gcctgttcgc tcatgatcgt tcttccttga
     3721 accagtttgg cttgaaacag tttggccgcc cgcgcgtcgt gcggcggctt cttgccggat
     3781 cgtagtcatg caccctattt gaaacggccc gccagcgcca acgcctgtct caaaatcgac
     3841 aagaaatcca atagatttgc ggtcgctttc tttttgcagg aagtcgcaaa tcagctatgg
     3901 catagcttca cctggggata cgtattgtcc aacgtttttt tagcccggcg agaggtcgct
     3961 cggcactatg gagctgaata gacatggccg acgacgagat catcctgtcc gagctttccg
     4021 acgacgaact cgtgcagcaa atgcacgacg acctctatga cggattgaaa gaggaaatcg
     4081 aggaaggcac caacatcctt ttggggcgcg gctgggttcc ctacaaggtg ctgaccgagg
     4141 cgctggtcga aggaatgcgc atcgtcggcg aggatttccg tgacggcatc ctgttcgttc
     4201 ccgaagtgct gctgtcggcc aacgccatga aggccggcat gttcatcctg cgcccgctgc
     4261 ttgccgccac cggcgcgccc aagcagggca agatggtcat cggcaccgtc aagggcgaca
     4321 ttcacgacat cggcaagaac ctcgtcggca tgatgatgga aggtgccggc ttcgacatca
     4381 tcgatctcgg catcaacaat tcggtcgaaa aatatctcgc tgccatcgaa gagcaccagc
     4441 ctgacattct cggcatgtcg gctctgttga ccacgaccat gccctacatg aaggtcgtta
     4501 tcgacacgat gaaggaaaag ggcatccgcg acgactatgt cgtcatggtc ggcggcgcgc
     4561 cgctcaacga ggaattcggc aaggccgtcg gcgcagacgc ctattgccgc gatgctgcgg
     4621 tagccgtcga aaccgccaag gacttcatga aacgcaagca caacgttcgt gcaaatgcct
     4681 gaatgacgaa agggccgccc ggttggagcg gccctttcgg ttcatagctg acaaggcgca
     4741 ggaaggcctt gacgctgcct aaccagctta atcgccggcc agcctcaatt agtcgagttg
     4801 gcgattttct tgccggcgtg ctgcgtggcg ttcaccgtat tggcggtgtc ctggccggcc
     4861 ccgcggatgg tatttgcgca gcccgtcacc agcacaaggc tggcaatggc ggcgatcgat
     4921 gcatatctgg atagcgtcat ggtaggtggt ccctctatga ataatctggc tatcgaaaac
     4981 ggcgaagtgg cgaactggtt ccatgaagcg cgcggatgaa gttgccgagg ccgaaaaagc
     5041 cgccaggtcc gggcggcttc tggtcatcgc ctgcggcatg cttgcgcgcg aaatcctggc
     5101 ggtaaagcaa cagctcaagc tcgatcatct cgatctcacc tgcctgcccg ccgactttca
     5161 tttctacccg gaccgcatcg cgccagctat ggataaggcg atcaccgaag ccagggcggg
     5221 cggctacgac catatcttcg tcggctacgc cgattgcggc accggcggca atctcgacca
     5281 tatttgcgag aaacatggcg tcgagcgcat ggccggccca cactgctttg ccttctacca
     5341 gcgcatggag gcttacaggg aggtcgcgga agacgacatg atgtcgttct acatgaccga
     5401 tttcctgtgc cgccagttcg acgctttttt catgaagccg ctcggcctcg acaagcaccc
     5461 cgagcttatc caggattatt tcggcaatta tgaaaagctg atctatctgg cccagaccga
     5521 cgacccggaa ctggacaagg tagccgaagc cgccgcgaag atgctggggc ttgtctatga
     5581 acgccgcccc accggctacg gcgacctccc ggcgggactt gcgcgggcgg ccgcttccgt
     5641 cgtctagttc ttcgaccaag gagaacgcga tgaacctgcg caaggccacc ctcaccgcct
     5701 gcattgccct ttttgccgga agcgcgcttg ccgacggcgt ggtgcagaag cttatcacgc
     5761 ccgccgacaa ggcgaggctc gaccactatg tcgagacgcg caagacggcg cttgatgagg
     5821 ccaaagccgg cagtccggcg gatgtgaaag cgcttaatgc gttgctggcg cggcggctcg
     5881 tttccttttc cgacaaggac ctgaccggca actggcaatg ccgcacgatc aagcttggcg
     5941 gcgatctcgg cgcactggtg atttacggct ggttcgaatg caaggtaacc gacgacggct
     6001 ccggctggct tttggaaaag accagcggct cgcagcgcac caagggccgc ttctacgacg
     6061 acggcgagaa acgctcgatt tatcttggtt cgttctacgt ttcgggcgat ccggtgaaac
     6121 cttatgccag cggccccggc accgatcagg tcggctacgc cttccgcacc gatgccaatg
     6181 agtttcgcat cgagtttcc
//
LOCUS       DASYCS010000018         4370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2757244,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000018 DASYCS010000000
VERSION     DASYCS010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4370
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2757244"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="VGM46_00735"
     CDS             complement(<1..468)
                     /locus_tag="VGM46_00735"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002967888.1"
                     /note="catalyzes the formation of 3-phosphonooxypyruvate
                     and glutamate from O-phospho-L-serine and 2-oxoglutarate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine aminotransferase"
                     /protein_id="HEY4191147.1"
                     /translation="MTTPTKPGLRPENPNFSSGPCSKRPGWSVEALKDAPLGRSHRAK
                     VGKSKLAQAIDLTREVLQVPADYRIGIVPASDTGAVEMALWSLLGERGVDMVAWESFG
                     SGWVTDVVKQLKLADTRKIEAPYGVLPDLKQIDFSRDVVFTWNGTTSGVRVPNA"
     gene            783..1520
                     /locus_tag="VGM46_00740"
     CDS             783..1520
                     /locus_tag="VGM46_00740"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006466068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY4191148.1"
                     /translation="MCLAPIPILTYHQIAIPAKRGSPFRSLTVHPGRFRRHMRWLARL
                     GYRGLSMRDLMPYLLGQQRGKVFGLTFDDGFQNVSENVLPVLAETGYTATNYFVAGHP
                     GGSNFWDRDKGVPEAPLMSARQISEWAGAGHEVGAHTVDHVDLPQLEESDAAAQIRMS
                     RVLLEELVGVPVSSFCYPYGKYGPEHVRIARMAGFSTATTTVRGHVRHGADMWQLPRV
                     PVVRSTSFLRLAQKLFTSYEDRRGRGA"
     gene            1513..2694
                     /locus_tag="VGM46_00745"
     CDS             1513..2694
                     /locus_tag="VGM46_00745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY4191149.1"
                     /translation="MPETGKPPRIAVIVRRFGAQFGGLEAYAENLVRELRHEYDLHVF
                     CQEWESDLPVPHTIVPRIEGAPSWLNLLDFTWRCQKLAKGFDIVHSHENSWLGDVHSV
                     HMMPVRYSRYHHRAGWKRSLSTWMNPRLLVYLTMEAMRYRKKAKRRIVAVSPLTLEQI
                     EIAYRRHPPATIIAPAVHVPQTEMSRGEGLDALGLPHDRHHALLVAHDPARKGLTTLL
                     AALPLVSAKVDLIVVGGRDDLAIRNRALVAAAGQAGRVHIWPRQKDLGVFYAAADVCV
                     FPTTGDAFGMVPLEAASHGCPTIVSSKRHCGFAHYVTPEIDALVLENPTDPVALAGAI
                     DRLFTDPALYERVARNGKSLALGMGWDRAAQSYSRLYQTILAGEEPGTTALPIDHRLA
                     T"
     gene            complement(2905..3753)
                     /locus_tag="VGM46_00750"
     CDS             complement(2905..3753)
                     /locus_tag="VGM46_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008596551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein"
                     /protein_id="HEY4191150.1"
                     /translation="MKLDIRIALALAATAIMPLAPALAADYDPPIYVDQAPEYKPVEI
                     GSGWYLRGDVGYILKDRIGNPGYRAFDATPPGSYSDNAFATSGIDTDVTFGAGFGYHF
                     NDWLRADATVEGFRGDFAGTTAGAGPCAPGLPASTGCRTEDGAKFSAIGVMANGYVDL
                     GTYAGFTPYVGGGLGYSLVRWDNLSNTAYCTGAACPTAAAIGTSQHEGEDSWRFTYAL
                     MAGVSYDISKNLKLDAGYKYRHIQGGDMFGWDSDTAAAGATGVQGRDDGFDTHEIKVG
                     LRYDLW"
     gene            complement(4029..>4370)
                     /gene="glmM"
                     /locus_tag="VGM46_00755"
     CDS             complement(4029..>4370)
                     /gene="glmM"
                     /locus_tag="VGM46_00755"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018430017.1"
                     /note="catalyzes the conversion of glucosamine-6-phosphate
                     to glucosamine-1-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEY4191151.1"
                     /translation="FSTTGDGLVSALQVLACVKRQDKPVSELSKKFEPVPQLLKNVRF
                     SGGKPLEDASVKAAIEEARNRLGDTGRVVIRPSGTEPLIRVMAEGDDESLVEKVVNDL
                     VGIISETRNAA"
BASE COUNT          809 a         1310 c         1419 g          832 t
ORIGIN      
        1 ggcgttcggc acacgcacgc cggaggtggt gccgttccag gtgaacacca cgtcgcgcga
       61 gaaatcgatc tgcttcaggt ccggcagcac gccatagggc gcttcgatct tgcgcgtgtc
      121 ggcaagcttg agctgcttga ccacatcggt gacccagccg gagccgaagc tttcccaggc
      181 taccatgtcg acgccacgct cgccgagcag cgaccacagc gccatttcaa cggcgccggt
      241 atccgatgcc ggcacgatgc cgatgcggta gtcggcggga acctgaagga cttcacgggt
      301 gagatcgatg gcctgagcca gcttggactt gccgaccttg gcgcggtgcg aacggccaag
      361 cggagcatcc ttcagcgcct cgaccgacca gccggggcgt ttggagcagg gacctgagga
      421 aaaattgggg ttttccggac ggagtccggg cttggtgggt gtcgtcatcg tggtctatcc
      481 ttccagatag tggcccctcg ttggggaggg gtggcccact ccggccatta ccgaaatccc
      541 cggaaaagcg caagcgataa aaggattgca ggtgcaatcc tgtaggtcgc tgtagctact
      601 tgctgaacaa agctggtaca tgcaggatga accgacacgc cagttacacg ctggcggccg
      661 tgacgtttcc tgattgtttg gtgcctatgc caagactaga cgaagagacc cattcggaac
      721 cgacacggtt cgatgaagcg ctgccgctcg gcccgcaatc cagcagcccg gtcgaacgcg
      781 ccatgtgcct ggctcccatt ccgatattga cctatcacca gatagccatt cccgctaagc
      841 gcggcagccc cttcagaagc ttgaccgtac atcccggccg tttccgcagg catatgcgct
      901 ggctagcccg cctcggctat cgcgggctgt cgatgcgcga tctgatgccg tatctgctcg
      961 gtcagcagcg cggcaaggtg ttcgggctga ccttcgacga cggttttcag aatgtgtccg
     1021 aaaatgtgct gccggtcctt gcggaaaccg gttacacggc aaccaattac ttcgtcgccg
     1081 gccatcccgg cggttcgaat ttctgggaca gggacaaggg cgttcccgaa gcgccgctga
     1141 tgagcgctcg acagataagc gaatgggccg gggcaggaca tgaggttggg gcgcatacgg
     1201 tcgatcatgt cgatttgccg caacttgaag agagcgacgc cgccgcgcag atacgcatgt
     1261 cgcgcgtgct gctggaagaa ctggtcggcg tgccggtcag ctcgttttgc tatccttatg
     1321 gaaagtatgg accggagcat gtccggatag cccgcatggc cggtttttca accgcaacca
     1381 ccaccgttcg cggccatgtt cgccatggcg ccgatatgtg gcagttgccc cgcgtgccgg
     1441 tggtgcgctc caccagcttc ctgcggctgg cccagaaact gttcacatcc tacgaggacc
     1501 ggagaggccg cggtgcctga gacaggaaag ccgccccgga tcgcggtcat cgttcgacgg
     1561 ttcggcgcgc agttcggcgg cctcgaagcg tatgcggaaa acctcgtccg tgaacttcgc
     1621 catgaatatg acctgcatgt tttctgccag gaatgggaga gcgacctgcc ggtcccgcac
     1681 accatcgtgc ctcgcatcga aggcgcgccg agctggctca atcttctgga tttcacctgg
     1741 cgttgccaga agctggcgaa aggtttcgac atcgtccatt cccacgagaa cagctggctt
     1801 ggcgatgtgc acagcgtgca catgatgccc gtgcgctatt cacgatacca ccatcgagcg
     1861 gggtggaagc gaagcctttc aacctggatg aacccgcgcc tgcttgtcta tttgacgatg
     1921 gaagccatgc gctaccgcaa aaaagccaag cgccggatcg ttgcggtttc gccgcttact
     1981 ttggaacaga ttgaaattgc ctacaggcgg catccgccgg cgaccatcat cgccccggcg
     2041 gtgcatgttc cccagaccga aatgagccga ggcgagggcc ttgacgccct tgggctgccg
     2101 catgacaggc atcatgcgct gctggttgcg cacgatccgg cacgcaaggg tttgacgacg
     2161 ctgctggcgg cattgccgct ggtaagcgcc aaagtcgatt tgatcgtggt gggcgggcgc
     2221 gacgacctcg ccattcgcaa ccgcgccctg gtggcggcgg ctggacaagc ggggagggtc
     2281 cacatctggc ctcgccaaaa ggacctgggc gtgttctacg ccgccgccga tgtttgcgtc
     2341 ttcccgacca ccggcgatgc gttcggaatg gtccctttgg aggcggcatc ccacggttgt
     2401 ccgacgatcg tcagctcgaa acgccattgc ggcttcgcgc attatgtgac gccggagatc
     2461 gatgcgcttg tgctggaaaa cccgaccgat ccggtcgcgc tcgccggagc catcgatcgg
     2521 ctgttcacgg acccggcgct ctatgaacgc gtggcgcgca atggaaagag cctcgctttg
     2581 ggcatgggct gggacagggc ggcccagagc tattctcggt tataccagac aatacttgcc
     2641 ggcgaggaac cgggcacgac ggcgctgccc atcgatcacc ggctggcgac ataggagacg
     2701 ggcgcagggc atgatgcctt caccgcttgc gcgggcatcg acccgcttgc cgagcgagcc
     2761 tgtcggaagt ctgtgctgga cgacgatcca ctccctcttt gccgaagagg gcgtgggcag
     2821 gatagtcgtt tcggcatccg ctccgcaaac gaaagcggcg gacccgaagg cccgccgcga
     2881 tgttttttcc gaagcggtag aaacctacca gaggtcgtag cgcaggccga ccttgatttc
     2941 atgcgtgtcg aaaccgtcat cacggccctg gacgccggta gcgccggcgg cagccgtatc
     3001 gctgtcccat ccgaacatgt ctccgccctg gatgtggcgg tatttatagc cggcatcgag
     3061 cttgaggttt ttggagatgt cgtaggagac gcccgccatc agcgcatagg tgaagcgcca
     3121 gctgtcttcg ccttcgtgct ggctagtgcc aatcgcggct gcggtcgggc aggccgcgcc
     3181 ggtgcaatag gccgtattgg aaaggttatc ccagcgcacc agcgaatagc cgaggccgcc
     3241 gcccacataa ggcgtgaagc cggcataggt gccgagatcg acgtagccgt tggccatgac
     3301 gccgatggcc gagaatttgg cgccgtcttc cgtccggcag ccggtcgagg cgggcaagcc
     3361 cggcgcgcac ggaccggcgc cggcggtggt gccggcgaaa tcaccgcgga acccttccac
     3421 ggtggcgtcg gcgcgcagcc agtcgttgaa atgatagccg aagccggcgc cgaaggtgac
     3481 gtcggtgtcg atcccgctgg tggcgaaggc attatcggaa tagctgccgg gcggcgtggc
     3541 atcgaaggcg cggtagccgg ggttgccgat ccggtccttg agaatgtagc cgacatcgcc
     3601 cctgagatac cagccggagc cgatttccac cggcttgtat tccggcgcct ggtcgacata
     3661 gatcggcggg tcgtagtcgg cggcaagggc tggcgcgaga ggcatgatgg cggtggcggc
     3721 aagcgccagc gcgatacgaa tgtcgagttt catggtcctc aactccagaa gttgccgctg
     3781 tttgcgaggc gaatacagcg tcggttcgtg gagtattgtt aaccatagtg gttaagtacg
     3841 ggttaaggct aacagagtgt tagcgatcaa tgatgtggca tgtcgaaaag ctgcgcaatc
     3901 acgggcgggc ttgcgttgca aacgagcgcc gccgggtatc gtcagtctgt tttaccgatc
     3961 cggcccggga tcagccggcg cgggcggccg ggcggaaatg tccgccgccg gtccggatca
     4021 tcgctggctc aggctgcgtt gcgggtctct gaaatgatcc cgacgaggtc gttgaccact
     4081 ttttcgacca ggctctcatc gtcgccctcc gccatgacgc ggatgagcgg ttcggtgccg
     4141 gagggacgga tgacgacgcg gccggtatcg cccaggcggt tgcgcgcttc ctcgatggcc
     4201 gccttgacgg aagcgtcttc cagcggcttg ccgccggaaa agcgaacgtt cttgagcaat
     4261 tgcggcaccg gctcgaattt cttcgaaagt tcgcttaccg gcttgtcttg ccgcttgacg
     4321 caggccaaca cctgcaaggc cgaaaccagg ccgtcgccgg tggtggagaa
//
LOCUS       DASYCS010000019         2169 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_727715,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000019 DASYCS010000000
VERSION     DASYCS010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727715"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VGM46_00760"
     CDS             <1..252
                     /locus_tag="VGM46_00760"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEY4191152.1"
                     /translation="SALGCAVQTLLAKGEAYTTAEFKVNLTRPITAKTGEVVCEGKVI
                     HKGRTLALSEATLKDKDGRLLAFGTETCSIFPVANLAAR"
     gene            complement(302..1015)
                     /locus_tag="VGM46_00765"
     CDS             complement(302..1015)
                     /locus_tag="VGM46_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191153.1"
                     /translation="MFGTGLLVAAIVWMFSGLWLAVSLLRLALPRWRRSAVRQAGFAA
                     ALFVLSLAAVLACNQFVYLDYGLTSRAGLEAARAAEKAANAAERAIADAKAQQAREAT
                     AKAKTEADARQAMLDEKQAALDAKQAKEAEATQAATDAENKAAALARRCKEKTLAFVM
                     SQTFVKRQLKAPSSAEFPWITDDQVAISTRPGCVFHVSAWVDAQNGFGAQIRSRYVVD
                     LKYLDDDAGSWQLIDIRIK"
     gene            complement(1092..1724)
                     /locus_tag="VGM46_00770"
     CDS             complement(1092..1724)
                     /locus_tag="VGM46_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018427065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD family hydrolase"
                     /protein_id="HEY4191154.1"
                     /translation="MMADRTGAPARAWQRMLSGRRLDLLDPSPLDIEITDIAHGLARV
                     ARWNGQTRGDHAFSVAQHSLLVEKLFGEIAPEASPETRLAALLHDAPEYVIGDMISPF
                     KSVMGGSYKQCEERLQRAIHLRFSLPAAINAALRRDIKRADQIAAYFEATMLAGFSIT
                     EATEFFGRPRGFSADRFDLTPRSVVTAQGEFLKRFSAIEGERARQGVTGR"
     gene            complement(1721..>2169)
                     /locus_tag="VGM46_00775"
     CDS             complement(1721..>2169)
                     /locus_tag="VGM46_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006331264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HEY4191155.1"
                     /translation="GGDLSSWNALRIAHGVAESGADYALGDAFPHDVLLDQTGGVGFS
                     KGCYVGQEVVSRMQHRGTARRRVLILSADTPLPGSGTEIAAAGKAVGVLGSTAGRQGL
                     AIMRIDRVKEALDAGVAITASDVPVTVAIPNWATFTFPQPAGTEEA"
BASE COUNT          398 a          707 c          678 g          386 t
ORIGIN      
        1 tcggcgctcg gctgcgcggt gcagacgctg cttgccaagg gcgaggccta cacgaccgct
       61 gaattcaagg taaatctgac ccggccgatt acggcgaaga ccggcgaagt ggtgtgcgag
      121 ggcaaggtga tccacaaggg gcgcacgctg gccctgtcgg aagcgacgct caaggacaaa
      181 gacggcagac tgctggcctt cggaacggaa acatgctcga tcttcccggt cgccaatctg
      241 gccgcccgtt gacgaagccc tgagccggag ccgccgtttt caatgcaaac gggctgccgc
      301 ctcattttat gcggatgtcg atcaattgcc agctgccagc atcgtcatcg agatatttga
      361 gatcgacgac atagcgtgag cggatttgcg cgccgaagcc gttctgggca tccacccatg
      421 cgctgacgtg gaaaacgcag ccgggcctgg tcgagatcgc cacctgatcg tcggtgatcc
      481 atggaaattc ggcgctcgaa ggcgccttca gctgccgctt cacgaatgtt tgcgacatga
      541 cgaaggccag cgtcttttcc ttgcacctcc tggccagcgc tgccgccttg ttttccgcat
      601 ctgtggctgc ctgcgtcgcc tcggcctcct tcgcctgttt ggcgtcgagg gcggcctgct
      661 tttcatcgag cattgcttgt ctggcgtccg cctcggtctt tgccttggct gtggcctcgc
      721 gggcttgctg cgccttggcg tcggcaatgg cccgttcggc ggcgttcgcc gctttctccg
      781 ccgctcttgc cgcctccagc cccgcgcgcg aggtcaggcc atagtcgaga tagacgaact
      841 ggttgcaggc aaggacggcc gccagcgaaa gcacgaaaag ggcagccgca aatcccgcct
      901 gacggacagc gcttctgcgc catcgcggca gcgccaacct caacagcgaa acagccagcc
      961 acaacccgga aaacatccag acaatggccg ccacgagcag cccggtccca aacatgcccc
     1021 accctcggtt ccccgaggat agtttggctc aaaatccgat atgtgcaaac gaaattgcgt
     1081 ctcgaagcgc cttagcggcc tgtcacgcct tgtctggctc gctcgccttc gatcgccgaa
     1141 aaccgcttca ggaattcacc ttgtgcggtg acgactgagc gcggcgtcag gtcgaaacgg
     1201 tcggcggaaa agccgcgcgg ccgaccgaag aactccgtcg cctcggtgat ggaaaacccc
     1261 gcaagcatcg tcgcctcgaa ataggcggca atctggtcgg cgcgctttat gtcgcgccgc
     1321 aaggcggcat ttatcgcggc aggcaaggaa aagcgcaggt ggatcgcccg ttggaggcgt
     1381 tcctcgcact gtttgtagct gccccccatc accgatttga acggtgagat catgtcgccg
     1441 atgacatatt ccggcgcgtc atgcagcagg gccgcgagcc gcgtttccgg cgaggcttcc
     1501 ggcgcgattt cgccaaacag cttttccacc agcagcgagt gctgggcgac ggaaaaggca
     1561 tggtcgccgc gcgtctggcc gttccagcgg gcaacgcggg caaggccatg ggcgatgtcg
     1621 gtgatttcga tgtcgagcgg cgagggatcg agcaggtcga gccgccgtcc cgacaacatg
     1681 cgctgccagg cgcgcgccgg cgcgccggtg cggtcggcca tcaggcctcc tccgtgccgg
     1741 ccggctgcgg aaaagtgaag gtcgcccaat tggggatggc gaccgtcacc ggcacatcgc
     1801 tggcggtaat cgcaacgcct gcgtcgagcg cctccttgac ccggtcgatg cgcataatgg
     1861 caaggccctg cctgcctgcc gtcgagccaa gcacgccgac tgccttgccg gcggcggcga
     1921 tctcggttcc gctgccggga agaggggtgt cggctgaaag gatcagcacc cgccgccgcg
     1981 ccgtgccgcg atgctgcatg cgcgaaacga cttcctgtcc gacataacag cctttgctga
     2041 aaccgacacc gccggtctgg tccagcagca catcatgcgg gaacgcatcg cccagcgcat
     2101 aatcggcgcc gctttcggca acgccatggg cgatgcgcaa cgcgttccac gaagaaagat
     2161 cgccgccat
//
LOCUS       DASYCS010000020        16378 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4351675,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000020 DASYCS010000000
VERSION     DASYCS010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 16378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16378
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4351675"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="VGM46_00780"
     CDS             <1..886
                     /locus_tag="VGM46_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008875055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY4191156.1"
                     /translation="DLQNAMLAEIAGIPEIELVTGARLSTLATHSRGITASFLVEGNL
                     KQANGFLMIGADGVWSKTRGLGKRPATSAFTGKLAWRATIAAESPAGAAFAKIATADC
                     VTAFLHPGFHLVAYPMRGGGAFNLVAFTEGKQMAQGWSGDADPAILGRAMRGAAPALA
                     DLARDAGPWKAWPVHTVDRACRWTSPEGIALIGDAAHAMPPFAAQGAAMAIEDAVTLA
                     ANVASLPANLPAALAAWEKSRKRRVARVARRGALNYLAWHAAGPVALARNLLLKARRA
                     EKLAADLDWLYGWRAPER"
     gene            complement(929..1732)
                     /locus_tag="VGM46_00785"
     CDS             complement(929..1732)
                     /locus_tag="VGM46_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006332113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEY4191157.1"
                     /translation="MAVENAVGAEEIKVNAEKMQISDTDVAIEIVAMHKWYGEFHVLK
                     DINLKVMRGERIVICGPSGSGKSTMIRCINRLEEHQKGTIKVDGKELTNDLKKIDEVR
                     REVGMVFQHFNLFPHLTILENCTLAPIWVRKTPKKEAEEIAMHFLKRVKIPEQAHKYP
                     GQLSGGQQQRVAIARSLCMNPRIMLFDEPTSALDPEMIKEVLETMVGLAEEGMTMLCV
                     THEMGFARKVANRVIFMDAGQIVEQNTPAQFFDHPQHERTKLFLSQILH"
     gene            complement(1760..2914)
                     /locus_tag="VGM46_00790"
     CDS             complement(1760..2914)
                     /locus_tag="VGM46_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008504345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY4191158.1"
                     /translation="MQEHDLSFVRAEMVIAQAAPKSEAGLVAWIRKNLFASVGDTVLT
                     IAGLLLVAYIIPPFLEWAFVNAQWTGTDRSFCATAAQGGIQPDGWSGACWAFVQAKFG
                     QFMFGRYPIDERWRPMLVAVMFVALFVPLLIPKVPRKGLNAIIFFIVFPIVAFVLLTG
                     GWFGLPHVETPLWGGLLVTLVLSFVGIAVSLPFGVLLALGRRSKMPVVKLVCVVFIET
                     VRGIPLVTVLFMASVMLPLFLPAGVTFDKVLRALIGVAMFASAYMAEVVRGGLQAIPK
                     GQYEGADSLGLGYWQKMILVVLPQALKLVIPGIVNTFIGMFKDTSLVYIISMFDLLGV
                     VRQNFSDATWASPQTPISGLVFAALIFWLFCFGMSRYSMFMERRLDTSHR"
     gene            complement(2915..4105)
                     /locus_tag="VGM46_00795"
     CDS             complement(2915..4105)
                     /locus_tag="VGM46_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004683531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY4191159.1"
                     /translation="MASQDILDGDKSSRGSFINDPKVRGIFFQVLVILLLVAGAWWIV
                     HNVVDNLRRSNIASGFSFLRSRAGFDISESPIAYTSDSTYGRAILVGFLNTLIVAAAG
                     IVTASIIGFVVGIGRLSRNWLIRKICTVYVELFRNIPPLLVIFFWYFGVLSVLPGSRD
                     SLHLPFGTFLNNRGFYFPRAIWGEGSWLIPVALVVGVALAWFVARRARQRQMATGQPF
                     PVFWTSVALIIGLPVLAFALNGFPLSFDMPKQSTFNLSGGFQVKPEFLSLYLALSFYT
                     AAFIAEIVRAGIIGVSKGQTEASEALGLRPRQILRLVVVPQAMRIIIPPLTSQYLNLT
                     KNSSLAIAIGYPDLVATGGTVLNQTGQAIEIVVIWMVIYLAISLATSAFMNWFNAKMA
                     LVER"
     gene            complement(4172..5200)
                     /locus_tag="VGM46_00800"
     CDS             complement(4172..5200)
                     /locus_tag="VGM46_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006332109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY4191160.1"
                     /translation="MKHIAIGILGAATLGLMAAAASASTLDDVKAKGFIQCGVSTGLA
                     GFSAPDDKGEWKGLDVDFCRAVAAAVFGDGTKVKFTPLSAKERFTALQSGEIDILSRN
                     TTWTINRDTALGLNFAGVTYYDGQGFMINAKKLPGVNSALQLSGAAVCVQAGTTTELN
                     LADYFKSNKMEYNPVVFEKLEEVNAAYDSGRCDVYTTDQSGLYGIRLTLGAPDDHVVL
                     PEIISKEPLGPAVRQGDDQWYHIVRWTYYALVQAEELGITQANVDEMKNSPNPEIKRV
                     LGTEADTKIGTDLGLTNDWVVNIVKATGNYGELFERNVGTGSPLKIARGLNALWTKGG
                     LQYAMPIR"
     gene            5616..6788
                     /locus_tag="VGM46_00805"
     CDS             5616..6788
                     /locus_tag="VGM46_00805"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008875048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEY4191161.1"
                     /translation="MAKNDIEMGTNTRLAHMGNNPRDYFGFVNPPVVHASTVLFPDTA
                     TLVSRAQKYTYGTHGTPTSDALAQACNELEGSAGTILVPSGLAAVTVPLLAFASAGDH
                     VLIVDSVYGPTRNFANTMLKRLGVDVEYYDPRIGAGIAALMKPNTTVVFTESPTSNTF
                     EVQDIPAITEAAHARGAIVMLDNSWATPLFFRPLDHGVDISIQAATKYPAGHSDVLLG
                     TVSAGESCWPKLWAAFIALGCCTGPDDAYQVLRGLRTMGVRLAHHQKSTLDIATWLEG
                     QPGIAQVLYPALPSHPDHALWKRDFSGASGVFSIVLDGGGQKQQHAFLDAFTIFGLGY
                     SWAGYESLAVPVSLGDHAIATNIYKGPLIRLQIGLENVEDLKADLSRGLAAMAAAK"
     gene            complement(6827..6903)
                     /locus_tag="VGM46_00810"
     tRNA            complement(6827..6903)
                     /locus_tag="VGM46_00810"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6867..6869),aa:Pro,seq:tgg)
     gene            7043..7348
                     /locus_tag="VGM46_00815"
     CDS             7043..7348
                     /locus_tag="VGM46_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006201534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ETC complex I subunit"
                     /protein_id="HEY4191162.1"
                     /translation="MSARIFSPAKTAMQSGKAKTGYWVLEFDPETPRKIDPLMGYTTS
                     SDMKSQIRLVFDTREEAVAYAEKQGLAFRIEEPKEAKRRQISYAENFRYDRRTPWTH"
     gene            7406..7482
                     /locus_tag="VGM46_00820"
     tRNA            7406..7482
                     /locus_tag="VGM46_00820"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7440..7442,aa:Arg,seq:tct)
     gene            complement(7569..8270)
                     /gene="hdaA"
                     /locus_tag="VGM46_00825"
     CDS             complement(7569..8270)
                     /gene="hdaA"
                     /locus_tag="VGM46_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006201540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator HdaA"
                     /protein_id="HEY4191163.1"
                     /translation="MAQQTEKPRQLPLDLGHGIGYSRDDLIVSSANEQAGALVDGWPD
                     WPSPVVVLAGPTGSGKTHLASIWRERADALQLTAQNLGDALRDIGGRAVLIDDIDAGR
                     IDEPALFHLINSVRGAGSHLLLTARRFPSAWQIDLPDLVSRLKAAATVEIHEPDDMLL
                     AGVITKLFADRQIEVEPHVVQYLSRRIERSLATAMRVVERLDRMALEQKTRITRTLAA
                     EAVNSMDEGQGEFDI"
     gene            complement(8275..9447)
                     /locus_tag="VGM46_00830"
     CDS             complement(8275..9447)
                     /locus_tag="VGM46_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006331906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY4191164.1"
                     /translation="MAKAAPPAPEELAAALAASVAFRRQISFWLIGAVVLALFLYVFS
                     DILLPFVAGMVLAYFLDPVADRLQRLGLSRIMATVVILVTFIVVLVLAFVILVPVLAT
                     QMADFAGKLPEYLTRLQALITSFDPQWLEEKFGVNAASLRDGLNSVLTSGFGLLTTVF
                     TSIWSSGVALVSVVSLFVVTPVVAFYMLLDWDRMVAKVDSWVPRDHVSTVRAIATDIN
                     TATAGFVRGQGTLCLVLGAMYAVGLTLTGLNFAILIGLFAGLISFIPYVGSLTGLVLA
                     VGVAFVQFWPDWTMVVAVAAVFFVGQFIEGNILSPKLVGKSVGLHPVWLMFSLFAFGA
                     LFGFVGLLIAVPASAAVAVLVRFAIARYLESPLYNGHGPQPAPPPTSRRKALPPRG"
     gene            complement(9419..9976)
                     /locus_tag="VGM46_00835"
     CDS             complement(9419..9976)
                     /locus_tag="VGM46_00835"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006331905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEY4191165.1"
                     /translation="MTIPNIITIMRLVLVPGVVLAMLQARWEWAFAGFVIAGVSDGID
                     GFIARHFNQRSRLGAYLDPIADKLLLVSVFVVLGIAGELPLWLVLAAVSRDALIVLAV
                     LLSSVMRHPVEVKPLLVSKANTVLQIVLAAVVLGELAFDIHTGTLRPALIMLSGLLTV
                     ASAAAYLVAWLRHMNGYGEGGTPGT"
     gene            10142..11206
                     /gene="purM"
                     /locus_tag="VGM46_00840"
     CDS             10142..11206
                     /gene="purM"
                     /locus_tag="VGM46_00840"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019170514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEY4191166.1"
                     /translation="MSDKKNGLTYADAGVDIDAGNLMVEKIKPLVRATRRPGADGEIG
                     GFGGLFDLKAAGFSDPVLVAANDGVGTKLRIAIEAGKHDTIGIDLVAMCVNDIVVQGA
                     EPLFFLDYFATGKLDPDQGAAIVSGIAEGCRQGGCALIGGETAEMPGMYHGSDYDLAG
                     FAVGAAERGQLLPTDDIVEGDVLLGLASSGVHSNGFSLVRRIVAKSGLDWNAPAPFAE
                     GVTLAEALLEPTKIYVKSVLKAIRNTHGIKALAHITGGGFPENIPRVLPKEFSAELDL
                     EAIDVPPVFSWLAKTGGVAAEEMMRTFNCGIGMVIVVASGQAAQVAAVLQEAGEKVEP
                     IGRIVPRRDAGVLYRGTIDL"
     gene            11203..11916
                     /gene="purN"
                     /locus_tag="VGM46_00845"
     CDS             11203..11916
                     /gene="purN"
                     /locus_tag="VGM46_00845"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008875039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEY4191167.1"
                     /translation="MTAPRKRVAILISGRGSNMTALIAAAADPDYPAEIVGVISDKAD
                     AAGLGMAAARGIPVQAIPRKDHASKTAHDDAIDAALSALRAEIVILAGYMRLLGASFV
                     EKWQGRIINIHPALLPSFKGLDTHKQALEAGARIHGCTVHFVTPLMDDGPIIAQAAVP
                     VLVGDSEADLTARVLKAEHRLYPLALKLVAEGKVRMEDGRAVFSGFADDADNASLLLT
                     APRPLESAVDLEQLARITP"
     gene            complement(11955..13832)
                     /locus_tag="VGM46_00850"
     CDS             complement(11955..13832)
                     /locus_tag="VGM46_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018427143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEY4191168.1"
                     /translation="MLTINDLSLRIAGRLLLDHASLVLPAGAKAGLVGRNGTGKTTLF
                     KAITGDLSSETGSVSIPKQTRIGQVAQEAPGTEEPLIEIVLKADVERTALLEEEKTAT
                     DPNRIAEIHIRLADIDAHSAESRAATILAGLGFDDAAQRRPASSFSGGWRMRVALAAV
                     LFSEPDLLLLDEPTNYLDLEGTLWLENYVSKYPHTVLLISHDRDLLNRAVNSIVHLDQ
                     KKLTFWRGGYDQFERQLSEQRELQEKGRVKQEAARKHLQSFVDRFRAKASKARQAQSR
                     IKALEKMKPISALVDDNVRPFHFPEPVKTVASPIVALERVDVGYQAGAPILKKMTLRI
                     DADDRIALLGANGNGKSTFAKLMSGRLKPETGSMTVAPGLKVAIFAQHQLDDLRPEEN
                     AYEHVRRLMPEAPESKVRGKVAQFGLATEKMNTPAKDLSGGEKARLLMGLAAFDGPNL
                     FILDEPTNHLDIDSRESLIHALNECPGAVILISHDRHLLEATADRLWLVKDGAVTPYD
                     GDLEDYKTLVTGVSGDRRERREADKASKADRRREAAARRAAFEPLAKEIRATEALMDR
                     LRKRADTIEDELADPALYEKDPIKATRLGKERSELSAQLKAHEDRWLTMSAEYEQGTA
                     E"
     gene            complement(13972..14670)
                     /locus_tag="VGM46_00855"
     CDS             complement(13972..14670)
                     /locus_tag="VGM46_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013525013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191169.1"
                     /translation="MTNTVARLTRSLAGGVMLIGLCAGANAQGLGVGVGASLGGDHGV
                     NAGVGASLGGGKGLGVGVGASAGGGNGVNAGLGASVGGGKGVNAGVGATAGGGNGVNA
                     GLGASVGGGNGVNAGLGASVGGGRGIDAGATASIGGGNGVGVGVGVGGGEPGGPTNPG
                     NPGNPSNPGNGGSASNPGTPGTNNPGLPGAVAHMSSRELARYKQRCADVMSGSGSPDR
                     SLRELCLLIQTASR"
     gene            complement(14859..15371)
                     /locus_tag="VGM46_00860"
     CDS             complement(14859..15371)
                     /locus_tag="VGM46_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY4191170.1"
                     /translation="MRRHFMMFAAYNQWANARLYDAAADLGDGEFSRDTGAFFGSMMG
                     TLNHILVADRIWMKRFTGEGDAPATLDTIIHKDLPALRGAREAQDKRIVDWVSMLDDQ
                     ALSGRFTYMTATDMRTISQRLSPALDHFFNHQTHHRGQAHMILTMNGRPSVQMDLSYF
                     QRTEEGRTYA"
     gene            15543..15965
                     /gene="ndk"
                     /locus_tag="VGM46_00865"
     CDS             15543..15965
                     /gene="ndk"
                     /locus_tag="VGM46_00865"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010661674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEY4191171.1"
                     /translation="MALERTFSMIKPDATRRNLTGAITKMLEDAGLRVVASRRVWMSL
                     REAQGFYAVHKDRPFFAELTEFMSSAPTIVQVLEGENAIAKNREVMGATNPANAAEGT
                     IRKVHALSIGENSVHGSDAPETAAEEIKYWFSDTEVVG"
     gene            complement(16052..>16378)
                     /locus_tag="VGM46_00870"
     CDS             complement(16052..>16378)
                     /locus_tag="VGM46_00870"
                     /inference="COORDINATES: protein motif:HMM:NF021158.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HEY4191172.1"
                     /translation="EQVMRGEVLYRERIALPPNAVLWVQLADVSKADAPAAVLSEQKI
                     NPAGQVPIKFEIRFDPSVIRVNMSYALQARITVDGKLWFVTDTRHPVDPLSDKPQTVL
                     VKRVAR"
BASE COUNT         3306 a         5221 c         4896 g         2955 t
ORIGIN      
        1 cgacctgcaa aacgccatgc tggcggaaat tgccggcata cccgaaatcg agcttgtcac
       61 cggcgcgcgg ctgagcacgc tggcgacgca ttcgcgcggc atcacggcgt cgtttctcgt
      121 cgagggcaac ctcaagcagg ccaatggctt cctgatgatc ggcgctgacg gcgtttggtc
      181 gaaaacacgc gggcttggca agcggccggc gacaagcgcc tttacaggca aacttgcctg
      241 gcgcgccacc atcgccgccg aaagtccggc cggtgccgcc ttcgccaaaa tcgcgacagc
      301 cgattgcgtc accgcattcc tgcatcccgg cttccatctg gtcgcctatc cgatgcgcgg
      361 cggcggcgcg ttcaacctgg tagccttcac cgaaggcaag cagatggcgc agggctggtc
      421 gggcgatgcc gatcccgcaa tccttgggcg cgcgatgcgt ggcgcagcac ccgctttggc
      481 cgatctcgcc agggacgccg gcccgtggaa ggcatggccg gttcacacgg tagaccgggc
      541 gtgccgctgg acatcgccgg aaggcatcgc cctgattggc gacgcggcgc acgccatgcc
      601 cccttttgct gcgcagggcg ccgcaatggc gatcgaggac gccgtgaccc tcgccgccaa
      661 tgtggcgagc ttgccggcca accttcccgc ggcccttgcc gcatgggaga aatcgcgtaa
      721 acggcgagta gccagggtgg cgcggcgcgg cgcgctcaac tacctcgcct ggcacgcggc
      781 cggccccgtc gcccttgccc gcaatttgct gttgaaagct cgccgggcag aaaagctcgc
      841 ggccgatcta gactggcttt acggttggcg cgcgcccgaa cggtgacccg gccgcttcgg
      901 tcgggcagcc cgaaaagaat attgccgatc agtgcaatat ctggctgagg aaaagcttgg
      961 tgcgttcgtg ctgcggatgg tcgaagaact gggccggcgt gttctgctcg acgatctgtc
     1021 cagcgtccat gaagatcacg cggttggcga ccttgcgggc aaaacccatt tcgtgggtca
     1081 cgcacagcat ggtcatgccc tcttccgcca gcccgaccat cgtttccagc acttccttga
     1141 tcatctccgg atcgagcgcc gaggtaggct cgtcgaacag catgatgcgc gggttcatgc
     1201 acagtgagcg cgcaatcgcc acgcgctgtt gctggccgcc ggaaagctgg cccggatatt
     1261 tgtgggcttg ttcgggaatc ttgacccgct tgaggaaatg catggcgatt tcctcggctt
     1321 ccttcttcgg cgtcttgcgc acccagatcg gcgccagcgt gcagttttcc agaatggtca
     1381 gatgcggaaa caggttgaag tgctggaaca ccataccgac ctcgcggcgc acctcgtcga
     1441 tcttcttcag gtcgttggtc aactccttgc catcgacctt gatcgtgccc ttctgatgct
     1501 cttccagccg gttgatgcag cggatcatcg tcgattttcc cgagcctgaa ggcccgcaga
     1561 tgacaatgcg ctcgccgcgc atcaccttca gattaatatc cttcaggacg tgaaactcgc
     1621 cgtaccattt gtgcatcgcc acgatctcga tggcgacatc ggtgtccgat atctgcatct
     1681 tctcggcgtt caccttgatt tcttcagcgc cgacagcgtt ctcaacagcc atgagccggt
     1741 tccttttttt gatctttttt catcgatgac tggtatcgag acgacgttcc atgaacatcg
     1801 aataacgtga catgccaaag cagaacagcc agaaaatcag ggccgcgaac accaggccgg
     1861 agatcggcgt ctgcggcgaa gcccaggtgg catccgagaa attctgccgc acgacgccga
     1921 gcagatcgaa catcgagatg atgtagacaa ggctggtgtc cttgaacatg ccgatgaagg
     1981 tgttgacgat gcccggtatg accagcttga gcgcctgcgg cagcaccacc aggatcatct
     2041 tctgccaata gccgaggcca agcgaatccg ccccctcata ctggcctttc ggaatggcct
     2101 ggaggccgcc gcgcaccacc tcggccatgt aggcggaggc aaacatcgca acgccgatca
     2161 aggcccgcag cactttgtcg aaagtcacgc cggccggcag gaaaagcggc agcatgacgc
     2221 tggccatgaa cagcaccgtg accagcggaa tgccgcgaac cgtctcgatg aagacgacgc
     2281 agacaagctt caccaccggc attttcgaac gccggccaag cgccagtagg acgccgaaag
     2341 gcaatgacac ggcgatgccg acaaaggaca gcaccaacgt aaccagaagg ccgccccaca
     2401 gcggcgtctc gacatgcggc aggccaaacc acccgccggt cagcaggaca aaggcgacaa
     2461 tcgggaatac gataaagaag atgatcgcat tcaagccctt gcgcggaacc ttgggtatca
     2521 gcagcggcac gaacagcgcc acgaacatca ccgcgacgag catgggccgc cagcgctcat
     2581 cgatcggata gcggccgaac atgaactgcc cgaatttcgc ttgaacgaac gcccagcagg
     2641 caccggacca gccatcaggc tggatgccac cctgcgcggc agtcgcgcaa aagctgcggt
     2701 cggtgcccgt ccactgcgcg ttgacgaaag cccactccag gaacggagga attatgtagg
     2761 cgaccaaaag caggccggcg atggtcagga cggtatcgcc gacggaggca aacaggttct
     2821 tgcgtatcca tgcgaccaaa ccggcctcgc ttttcggcgc ggcctgcgcg atcaccattt
     2881 cggcgcgaac gaaggaaaga tcgtgttcct gcatctatct ctccaccagc gccattttgg
     2941 cgttgaacca gttcatgaag gcggaggtcg caaggctgat tgcgagatag atcaccatcc
     3001 agatcactac gatctcgatc gcctggccgg tctggttcag caccgtgccg ccggtggcca
     3061 caaggtcggg atagccgatg gcgatggcaa gcgaggaatt cttggtcagg ttgaggtatt
     3121 ggctggtcag cggcggaatg atgatgcgca tcgcttgcgg caccaccacc agccgcagga
     3181 tttgccgcgg ccttagccca agcgcctcgg atgcctcggt ctgccccttg ctgacgccga
     3241 tgatgccggc gcgcacgatc tcggcgatga aagcagccgt atagaaggaa agcgccagat
     3301 agagcgacag gaattccggc ttgacctgga aaccgccgct caggttgaat gtcgattgct
     3361 tcggcatgtc gaagctcagc ggaaagccgt tcagcgcgaa ggcgagaacc ggcaggccga
     3421 tgatcagcgc cacggatgtc cagaataccg ggaatggctg cccggtcgcc atctgcctct
     3481 ggcgcgcacg ccgcgccacg aaccacgcca gcgcgacgcc caccaccaat gccacgggaa
     3541 tgagccacga accctcgccc cagatggcgc gcggaaaata aaagccgcga ttgttgagga
     3601 acgttccaaa cggcagatgc aggctgtcgc gtgaacccgg cagcacggaa agaacaccga
     3661 aataccagaa gaagatcacc agcagcggcg gaatattgcg gaacagttcg acatagacgg
     3721 tgcagatctt gcggatcagc cagttacgcg aaagccggcc gataccgacg acgaaaccga
     3781 tgatcgatgc cgtgacgatg cccgctgccg ccacgataag cgtgttgagg aaaccgacca
     3841 ggatggcccg gccataagtc gaatcggagg tgtaggcgat cgggctttcg gaaatatcga
     3901 agcctgcccg gctgcgcagg aacgagaagc ccgaagcgat attcgaccgc ctcagattgt
     3961 cgacgacgtt gtggacgatc caccaggccc cggcgaccag cagcagtatg accagaacct
     4021 gaaagaaaat gccgcgtacc ttcggatcgt tgatgaagga accacgactg cttttgtcac
     4081 cgtcaagaat atcttgcgat gccatgcgac ggtctccctg agagaggccg aaaggcggac
     4141 gataccgcct ttcggatcac gctccgatcg atcagcggat cggcatggcg tattgcaggc
     4201 cgcccttggt ccacagcgcg ttgaggccgc gggcgatctt gagcgggctg ccggtaccga
     4261 cattgcgctc gaacagttcg ccgtaattgc ctgtcgcctt aacgatattg accacccagt
     4321 cattggtcag gccaagatcg gtgccgatct tggtatcggc ttcggtgccg agcacgcgct
     4381 tgatctcggg attgggagag ttcttcattt catcgacatt ggcctgggta atgcccagtt
     4441 cctcggcctg caccagcgcg taataggtcc agcgcacgat gtggtaccac tggtcgtcgc
     4501 cctggcgcac ggcaggtcca agcggttcct tggagatgat ctcgggcagc acgacatgat
     4561 cgtccggcgc gccgagcgtc aggcggatgc cgtaaagccc cgactggtcg gtggtgtaaa
     4621 cgtcgcagcg gcccgaatca taggcggcgt tgacctcctc aagcttctcg aacacgacgg
     4681 ggttatattc catcttgttc gacttgaagt agtcggcaag gttgagttcg gtcgtcgtgc
     4741 cagcctgcac gcacaccgcc gcgcccgaaa gctggagcgc ggaattcacg ccgggcagct
     4801 tcttggcgtt gatcatgaag ccctgcccgt cataataggt cacgcccgca aaattcaggc
     4861 caagagccgt atcgcggttg atcgtccagg tcgtattgcg cgacaggatg tcgatctcgc
     4921 cggattgaag cgcagtaaat cgttccttgg cgctgagcgg cgtgaacttg accttggtgc
     4981 cgtcgccgaa cacggcagct gccaccgcgc ggcagaaatc gacgtccagc cctttccact
     5041 cgcccttgtc gtcgggcgcc gaaaaacctg caaggccggt agagacgccg cactggatga
     5101 aacccttggc cttgacgtcg tcaagcgtgc ttgcggatgc ggcggcggcc atcaatccga
     5161 gcgtcgctgc gccaagaatg ccgattgcaa tgtgtttcat gacccactga cccttttctt
     5221 ttattcaggc attacctgag tgttttccca agcgaacgca gcattcgttc cagcccactt
     5281 ctggaacgcg gcatcggggt agatgcatcg agcgttttcc gttcacgaca agcatcgaaa
     5341 gcgattattt tcgtgaggtc aagggaaatg accaaaccga aagcgtttgg aagccaaatc
     5401 cgtaaatgcg gccccgcaaa aaagcagcgg cccgcaaagc ggtgacgaac ccccaacggg
     5461 tctgcttcgc ttgccgcgat ctgcacgagg ttgcgtatcc gctattttgg cataggcaaa
     5521 cacagcctgc tggcaggccg cgaacggttg cgtcgccaag gccggcgcac tatgcctagc
     5581 ctctcttgat cgagcggcag gacgcgggca gaaccatggc aaaaaacgac atcgaaatgg
     5641 gcaccaacac gcggctcgcc cacatgggca acaaccctcg cgactatttc ggcttcgtca
     5701 acccgccggt cgtgcatgct tcgacagtgc tgtttccgga tacggcgacg ctggtttcgc
     5761 gcgcgcagaa atatacttac ggcacgcatg gcacccccac ctcggatgcc ctggcgcaag
     5821 cgtgcaacga actggaaggc tccgcgggca ccatcctggt tccgtccggc ctggccgccg
     5881 tcaccgtgcc gctgctcgcc ttcgcttcgg cgggcgacca tgtgctgatc gtcgattccg
     5941 tctatggtcc gacgcgcaac ttcgccaata cgatgctgaa gcggctgggc gtggatgtcg
     6001 agtattacga tccgcgcata ggcgcaggca tcgcggcgct gatgaagccc aacaccaccg
     6061 tggtgttcac cgaatcgccg acctccaaca ccttcgaagt gcaggatatt ccggcgatca
     6121 ccgaggcggc gcatgcgcgc ggcgcgattg tcatgctcga caatagctgg gcgacgccgc
     6181 tgtttttccg ccctctcgac catggcgtcg atatttcgat tcaggcggcg acgaaatatc
     6241 cggcgggcca ttccgacgtt ctgctcggca ccgtctcggc cggcgagtca tgctggccga
     6301 agctttgggc cgcgttcata gcgcttggtt gctgcacagg gccggacgac gcctatcagg
     6361 tgctgcgcgg cctgcgcacc atgggcgtgc gcttggcgca tcaccagaag agcacgctcg
     6421 acatcgcaac gtggctggaa ggccagccgg gcatagcgca ggttctctac cccgcattgc
     6481 ccagccatcc cgaccacgcc ctttggaagc gcgatttttc cggcgcaagc ggcgtcttct
     6541 ccatcgtgct ggacggcggt ggccagaaac aacagcacgc cttcctcgac gccttcacga
     6601 ttttcggcct gggctattcc tgggccggct atgaaagcct tgccgtgccg gtttccctgg
     6661 gggatcacgc gattgccacg aacatttaca aaggcccgct tatccgcttg cagatcggcc
     6721 tcgaaaatgt cgaggacctg aaagccgatc tttcgcgcgg cctcgcggcg atggcggcag
     6781 cgaaatagcc ggacggcccg tctgcatatt tggcgaatgt gggaaatggt cggagtggag
     6841 agattcgaac tcccgaccct ctggtcccaa accagatgcg ctaccagact gcgctacact
     6901 ccgatggccg ttgcctattg atttaggcga ggcaaagcaa gtgtaaaaaa ccacggctct
     6961 tgccgtcagg cgaattgggc ggtaagcacc gcacatcttg tgtagcggcg gcaatgcccg
     7021 ccgaaacgac aatgaggttt tcatgtccgc tcgcatcttc agccccgcca agaccgccat
     7081 gcagtccggc aaggccaaga ctggttactg ggtactggaa ttcgaccctg aaacgccaag
     7141 gaagatcgat ccgctgatgg gctacaccac ctccagcgat atgaagagcc agatccgcct
     7201 ggttttcgat acgcgcgagg aagctgtcgc ctatgcggaa aagcaaggcc tggccttccg
     7261 tatcgaagaa ccgaaagagg caaagcgccg ccagatttcc tatgcggaaa acttccgcta
     7321 tgaccgccgg acaccctgga cgcattgaac tgaaatccgc ccggtcgcgg caacgctatc
     7381 cgcggcgctt gcgtagcgat taaacggtcc cgtagctcag ctggatagag caccggcctt
     7441 ctaagccgat ggtcacaggt tcgaatcctg tcgggatcgc cactatctgt tggcggacaa
     7501 aggcaaagcc accactgtgg gaaataccgc cgcgctcaag tattcgcctg ctgcttttcg
     7561 acgaaagctc agatatcgaa ctcgccttgg ccctcgtcca ttgaattgac cgcttcagcg
     7621 gcaagcgtgc gcgtaatgcg cgtcttttgc tcaagcgcca tgcggtcgag ccgctccacc
     7681 acgcgcatcg ccgtcgccag cgaacgctct atgcgccgtg aaagatattg caccacgtgc
     7741 ggctcgacct cgatttgccg gtcggcaaac agcttggtta tgacgcccgc cagcagcatg
     7801 tcgtccggtt catggatttc caccgtggcg gcggctttca gccgcgaaac gagatcaggc
     7861 aagtcaattt gccaggcgga aggaaagcgc cgcgccgtca aaagcaggtg cgatccggcc
     7921 ccgcgcacgc tgttgatgag gtggaacagc gccggctcat cgatgcgacc ggcgtcgata
     7981 tcgtcgatca gcaccgcgcg cccgccaata tcccgcagcg cgtcgccaag gttctgcgcc
     8041 gtcagctgca atgcatcggc gcgctcgcgc cagatcgagg ccagatgcgt ctttccagag
     8101 ccggtcggcc cggccagcac caccacaggc gacggccagt ccggccagcc gtcgaccagt
     8161 gcgcccgcct gctcattggc gctggacacg atgaggtcgt cgcgcgaata gccgatgcca
     8221 tggccaagat cgagcggcag ttgacgcggc ttttccgttt gctgtgccat cctgtcagcc
     8281 gcgcggcggc agcgcctttc gccgcgatgt aggcggcggc gcaggctgcg ggccatgccc
     8341 gttgtagagc ggcgattcga gatagcgcgc aatggcgaag cgcaccagca cggccaccgc
     8401 ggcagaagct ggcaccgcaa tcaacagccc gacgaagccg aacagcgcac cgaaggcgaa
     8461 cagcgaaaac atcagccaga ccgggtgcag gccaacgctt ttgccgacca gcttcggcga
     8521 caatatgttg ccctcgatga actgcccgac aaaaaacacc gcagccacgg caacgaccat
     8581 cgtccaatcc ggccagaact gcacgaaggc gacgcccacc gccagaacga ggccggtcag
     8641 cgaaccgaca tagggaatga agctgatgag accggcgaaa aggccgatca gaatggcgaa
     8701 attcaacccg gtaagcgtca gcccgactgc gtacatcgcg cccagcacga ggcataatgt
     8761 gccctgcccg cgcacgaaac cggcggtcgc cgtgttgatg tcggtggcga tcgcccgcac
     8821 cgtgctcaca tggtcgcgcg gcacccagct atcgaccttg gccaccatgc gatcccagtc
     8881 gagcagcatg tagaaggcga cgaccggcgt caccacgaac aggctgacca cggaaaccaa
     8941 tgctacgccg gaactccaga tcgacgtgaa aaccgttgtc agcaggccga agccggaggt
     9001 cagcaccgaa ttcagcccgt cgcgcaggct ggcggcgttg acgccgaatt tctcttccag
     9061 ccattgcgga tcgaagctgg tgatgagcgc ctggaggcgc gtcagatatt cgggcagctt
     9121 gccggcaaaa tcggccatct gcgttgccag caccggcacc aggatgacga aagccagcac
     9181 cagcacgacg atgaaggtga cgaggatcac caccgtcgcc atgatgcgcg acaggcccag
     9241 ccgctggagg cggtcggcca ccggatcgag gaaataggcc agcaccatgc cggcgacgaa
     9301 cggcaagaga atatcgctga acacatagag gaacagcgcc agcacaaccg cgccgatcag
     9361 ccagaagctt atctggcgcc ggaaggctac cgatgccgcc agtgcggcgg caagttcttc
     9421 aggtgccggg ggtgccgcct tcgccataac cgttcatatg cctcagccaa gccacgagat
     9481 aggctgcggc ggaagccacg gtcaagaggc cggacagcat tatcagtgcc gggcgcagcg
     9541 tgcctgtgtg gatgtcgaat gcaagttcac cgagcacaac cgccgccaga acgatctgca
     9601 agacggtatt ggccttcgat acaagcagcg gcttcacctc gaccggatgg cgcatcacgc
     9661 tcgacagcaa tacggccaag acgatcaacg catcgcgcga gaccgcagcg aggaccagcc
     9721 acagtggcaa ttcacccgca atgcccagca ccacgaaaac gctgaccagc agcaatttgt
     9781 cggctatcgg gtcgagatag gcgccgagcc tggagcgctg gttgaaatgc cgggcgatga
     9841 agccatctat cccgtccgaa acgccggcga tgacgaagcc ggcaaacgcc cattcccagc
     9901 gcgcctgcaa catggccagc accacgcccg gcaccagcac aaggcgcatg atcgtgatga
     9961 tgttgggaat ggtcaccccg tcttcctcaa ttcattccgt tggttgcctg acataaatga
    10021 tcggcgcgaa cgcccacggc aagtcccgaa cgctgtccac ggttttattc gcccttaaag
    10081 cccgccgcct tgcgggacgc ggcggttcat gcgaagagcc gggacaagca ggaaaatggc
    10141 gatgagcgac aaaaagaacg ggctcaccta tgccgatgcg ggcgtcgata tcgatgccgg
    10201 caaccttatg gtggagaaga tcaagccgct ggtgcgcgcg acgcgccggc ccggcgccga
    10261 tggtgaaatc ggcggcttcg gcgggctttt cgacttgaag gcggcgggct tttcagaccc
    10321 ggttctggtt gccgccaacg atggcgtcgg caccaagctg cgcatcgcca tcgaggccgg
    10381 caagcacgat acgatcggca tcgacctcgt cgccatgtgc gtcaacgaca tcgtggtaca
    10441 gggcgccgaa cccctgttct tcctcgacta ttttgccacc gggaaactcg accccgacca
    10501 gggcgcggcc atcgtcagcg gcatcgccga aggctgccgg caaggcggct gcgcgctgat
    10561 tggcggcgaa accgctgaaa tgccgggcat gtatcacggc tcggactacg atcttgccgg
    10621 cttcgcggtg ggtgcggccg aacgcggcca gcttctgcct accgacgata tcgtcgaggg
    10681 cgacgtgctg ttgggcctcg cctcttccgg tgtccattcc aacggctttt cgctggtccg
    10741 ccgcatcgtc gccaaaagcg gccttgactg gaatgcgccg gcacctttcg ccgagggcgt
    10801 cacgctggcc gaagcgctgc tggaaccgac gaagatttat gtgaaatccg tcctcaaggc
    10861 gatccgcaac acgcacggca tcaaggcgct ggcccacatt accggcggcg gcttccccga
    10921 aaatatcccg cgcgtgctgc ccaaggagtt ttcggccgag cttgatctcg aagccatcga
    10981 tgtgccgccg gtcttctcgt ggctggccaa aacgggcggc gtcgccgccg aagaaatgat
    11041 gcgcaccttc aattgcggca tcggcatggt catcgtcgtg gcatccggcc aggcggcgca
    11101 ggttgcggcg gtcttgcagg aagccggcga aaaggtcgag ccgatcggcc gcatcgtgcc
    11161 gcgccgcgac gcgggcgtgc tctaccgggg cacgatagac ctatgacagc gccacgcaag
    11221 cgcgtcgcca tcctcatttc cgggcgcggc tccaacatga ccgcgctgat tgccgcggct
    11281 gccgatcccg attacccggc tgaaatagtc ggagtcatct ccgacaaggc ggacgccgcc
    11341 ggcctcggca tggccgccgc gcgcggcatt ccagttcagg cgatcccccg caaggaccat
    11401 gcaagcaaga cggcgcatga cgatgccatc gatgccgcgc tttcggcgct gagggcggaa
    11461 atagtcattc tcgccggtta tatgcggctg cttggtgcgt ctttcgttga aaaatggcag
    11521 ggccgcatca taaacatcca cccggcgctg ctgccatcct tcaaggggct ggatacgcac
    11581 aagcaggcgc tggaagcggg cgcgcgcatc catggctgca ccgttcactt cgtcacgccg
    11641 ctgatggatg acggcccgat catcgcgcaa gcggcggtgc ccgttctggt tggcgacagc
    11701 gaagccgacc tgaccgcccg cgtgctgaag gccgagcatc ggctctatcc gcttgcgctg
    11761 aaactggtcg ccgagggcaa ggtgcgcatg gaagacggcc gcgctgtctt ttccggcttt
    11821 gctgacgacg ccgacaatgc ctcactgctt ctcaccgcgc cccgcccgct cgaaagcgcg
    11881 gtcgaccttg agcagttggc gcggatcacc ccgtaatcgg tcccaccgcc tcacctctcc
    11941 ccggcaaaat cgccttactc tgccgttcct tgctcatact cagccgacat ggtcagccag
    12001 cggtcctcgt gggccttgag ctgcgccgaa agctcggagc gctccttgcc aagccttgtc
    12061 gccttgattg gatccttctc gtagagcgcc ggatccgcca gctcgtcctc gatggtgtcg
    12121 gcgcgcttgc gcaatcggtc catcagcgcc tcggtggcgc ggatttcctt ggccagcggc
    12181 tcgaaagcgg cgcgccgggc ggcggcctcg cggcggcggt ccgccttgga ggccttgtcg
    12241 gcctcgcgac gctcccgccg gtcgcccgaa accccggtaa ccagcgtctt gtagtcctct
    12301 agatcgccat cataaggagt gaccgcgccg tccttgacca gccacagccg gtcggcggtg
    12361 gcttcgagca gatggcggtc gtgcgagatc aagatcaccg cgcccggaca ctcgttcagc
    12421 gcatggatga gcgattctct ggagtcgata tcgaggtggt tggtaggctc gtcgaggatg
    12481 aacaggttcg gcccgtcaaa cgccgccaac cccatcaaaa gccgcgcctt ttcgccaccg
    12541 gacaaatcct tggccggcgt gttcatcttc tccgtcgcca ggccgaactg cgcgaccttg
    12601 ccgcgcactt tcgattccgg cgcttccggc atcagccggc gtacatgctc gtaggcattt
    12661 tcctccggcc gcagatcgtc cagctggtgc tgcgcgaaga tcgccacctt caggccgggc
    12721 gcaaccgtca tcgagccggt ttcgggcttc agccggcccg acatcagctt ggcgaaggtg
    12781 gacttgccgt tgccgttggc gccaagcagc gcgatgcggt cgtcggcatc gatgcgcagc
    12841 gtcatcttct tcaggatcgg cgcgccggcc tgatagccga catcgacgcg ctccagcgcg
    12901 acgatgggcg aagcaaccgt cttgaccggc tcgggaaagt ggaacggccg cacattatcg
    12961 tcgaccagcg ccgaaatcgg cttcatcttt tccagcgcct tgatgcgcga ctgtgcctgc
    13021 cttgccttcg aggccttggc ccggaaccgg tcgacgaagg attgcaggtg ctttcgcgcc
    13081 gcctcctgct tgacccttcc cttttcctgc aattcgcgct gctcgctcaa ttgccgctcg
    13141 aactggtcat agccgccacg ccagaaggtc agcttcttct ggtcgagatg aacgatggaa
    13201 ttgacggcgc ggttcaacag gtcgcggtcg tgcgagatga gcagcacggt atggggatat
    13261 ttcgacacgt agttctcaag ccacagcgtg ccttccagat cgagatagtt ggtcggctcg
    13321 tccagcagca gcagatcagg ctccgaaaac agcacagccg ccagcgccac gcgcatgcgc
    13381 cagccgccgg agaaggacga ggccggacgc cgctgcgccg catcgtcgaa gccgaggccg
    13441 gccaggatgg tggccgcgcg ggattcggcc gaatgcgcat cgatatcggc aagcctgatg
    13501 tggatttccg cgatgcggtt ggggtcggtc gccgtctttt cttcttccag cagcgccgtg
    13561 cgctcgacgt cggccttcag cacgatctcg atcagcggct cttccgtgcc cggcgcttcc
    13621 tgcgccacct ggccgatccg cgtctgcttg gggatgctga cagagcctgt ctcggacgac
    13681 agatcgccgg tgatggcctt gaacagcgtg gtcttgccgg tgccgttgcg gccgacaagc
    13741 ccggccttcg cccctgccgg cagcaccagc gaggcgtggt cgagaagaag gcggccggcg
    13801 atgcggagag aaagatcgtt gatcgtgagc atggcggggc ttttgcacgg gacgcagccg
    13861 aaccgcaagg cctacagtgc ctgcatgagt tgcagaggcc ggctgaaaat aaaaacgacc
    13921 cgctccaacg ggtcgctttc gatcggtatg gtccagtgaa atgcctcagc ttcagcggct
    13981 agccgtctgg atcagcaggc acagttcgcg caggctgcgg tccggactgc ccgaaccgct
    14041 catcacatcg gcgcagcgct gtttgtatct cgccaattcg cggctcgaca tatgggcaac
    14101 tgcacccggc aagccgggat tgttcgtgcc cggcgtgccg ggattggaag cgctgccccc
    14161 attgccggga ttggaggggt tgccagggtt gccgggattg gttgggccgc ccggttcgcc
    14221 gccacccaca ccgacgccga cgcccacgcc gtttccgcca ccgatagaag ccgtagcccc
    14281 ggcatctata cccctgccgc caccgacaga agcgccgagg cccgcattca cgccattacc
    14341 gcctccgacc gaggcgccaa ggcctgcatt gacaccgttg ccgcctcccg ctgtagcgcc
    14401 gacgccagca ttgacgccct tgccaccacc gactgaagcg ccaaggcctg cattgacgcc
    14461 gttgccgcca ccggccgagg cgccgacgcc gacgcccaaa cccttgccac cgcccaatga
    14521 cgcgccaacg ccagcattga cgccatggtc gccgccaagc gaggctccga caccaacgcc
    14581 aagaccttgc gcattggccc cggcacatag accgatcaac atcactccac cagccaagct
    14641 tctggtaagt cgtgcaactg tattagtcat aacaaactcc ctatagttgc cttagctaat
    14701 ataacacagt ctttatcaac tgcgtattca actacgaatg ggaggtgcat tcagtttcat
    14761 gacttagagc aaaagcaaat aatatggttg ttgaagctta acttcaaaac gtaaagtgaa
    14821 aaacgaaatt tctaaaaaat gaatctttca ggtcggcatc aagcgtaggt tctgccctct
    14881 tccgtgcgtt ggaaatagct gaggtccatt tgcaccgatg gccggccgtt cattgtcagg
    14941 atcatgtgcg cctggccgcg atgatgggtc tggtggttga aaaaatggtc gagggccggc
    15001 gaaagccgct gcgaaatggt gcgcatgtcg gtcgcggtca tataggtgaa gcggccggaa
    15061 agcgcctggt cgtccagcat cgacacccag tcgacgatcc gcttgtcttg cgcctcgcgc
    15121 gccccgcgca gcgccggcag gtccttgtgg atgatggtgt ccagcgtcgc cggtgcgtcg
    15181 ccttcgccgg tgaagcgctt catccatatc cggtccgcca ccagaatatg gttcagcgtg
    15241 cccatcatcg acccgaagaa cgcgccggta tcgcggctga actccccgtc gcccagatcg
    15301 gcggccgcat cgtagagccg cgcattggcc cattgattat aggccgcaaa catcatgaaa
    15361 tgccgtctca tcgataacct cgccgcttgc cggtatcccg gcgccatccc taagctctaa
    15421 gccgccaaac gccaataccg cctgtgtgcc ggcgacgcgc atgttcctgc gtcccccttg
    15481 gcaatgccgt gacctgcttg tatagagccg ccacttcaat cccatacgca caaggatatc
    15541 ccatggcgct cgaacgcacc ttttcgatga tcaagccgga cgcgacccgg cgcaacctca
    15601 ccggcgccat caccaagatg ctggaagacg ccggcctgcg cgtcgtcgct tcccgccgcg
    15661 tctggatgag cctgcgcgag gcgcagggct tctacgccgt ccacaaggat cgtcccttct
    15721 tcgccgaatt gacggaattc atgtcgtcgg ccccgacgat cgtgcaggtt ctggaaggcg
    15781 aaaacgccat cgccaagaac cgcgaagtga tgggcgccac caatccggcc aacgcggcag
    15841 aaggcaccat ccgcaaggtg cacgcgctat cgatcggcga gaactcggtc catggttccg
    15901 atgccccgga aaccgccgcc gaagagatca aatactggtt ctcggacacc gaagtcgtcg
    15961 gctgattcga aatcttgact ttttgaacga gatatctgtt caaaacaaaa atgccggggc
    16021 aacaccccgg ctttttcgtt tcgtgggagt gttagcgcgc cacccttttg accaggacgg
    16081 tctgcggctt gtcggatagc ggatcgaccg gatggcgcgt atccgtgacg aaccagagct
    16141 tgccgtcgac cgtgatgcgc gcttgcaggg catagctcat attcacccgg atgacggatg
    16201 ggtcgaacct gatctcgaac tttatcggca cctgccccgc cggattgatc ttctgctcgc
    16261 tgaggacggc cgcgggtgcg tccgccttcg aaacatcggc aagctgcacc caaagcacgg
    16321 cattgggcgg caacgcgatc ctctcgcggt aaagcacctc gccgcgcatg acctgttc
//
LOCUS       DASYCS010000021        16723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_583242,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000021 DASYCS010000000
VERSION     DASYCS010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 16723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16723
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583242"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /locus_tag="VGM46_00875"
     CDS             <1..821
                     /locus_tag="VGM46_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007068610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEY4191173.1"
                     /translation="MALWDIRGKAVGLPLYKLLGGGQRPIKAYAGGISLGYQEPASLA
                     REAHDMVSRGYRAIKLRIGDTPQRDIARISEVRKSIGDEIDILTDANANATMEHVRAI
                     MPSLDENRVGWLEEPFAAHDYRSYRQATRFGTTPLAAGENHYTRFEFHRLVEDGVITY
                     LQPDLSKAGGITEALRIAAMASAWKLSINPHCSATGLNMAATLHFLASIDNPGYFEAE
                     AASFNPFRDDLCSMSYKVGADGFVHVPEGPGIGIEVDEAFIARHPLIDGPGYVK"
     gene            complement(903..1814)
                     /locus_tag="VGM46_00880"
     CDS             complement(903..1814)
                     /locus_tag="VGM46_00880"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY4191174.1"
                     /translation="MTASQISLGAAGRARPPVRRNMDMADLHHVTSRLMIYLLVITVT
                     CVVGFPLFWMVICSFKTSGELYQFPPAFLPHQWTFTNYRDLFVQTNFPIYFMNSLIVA
                     GGATALSLVIGGLGAYSLSRFRFFGLGTFSLLTLICYMLPDVLLVIPLYIYVVKLGLV
                     DTLLSLIIANTAFTLPLTLWFMRSYFNAIPASLEESAMIDGCTRFQAMRRVTLPLALP
                     GLVSVGVFTFNHAWNEFLFALVFTSSETNKVLSLGLATWIGQDNIYSWGMLLAGAVLV
                     TIPVVVFYLLVQRNLITGLSAGGVKGE"
     gene            complement(1811..2707)
                     /locus_tag="VGM46_00885"
     CDS             complement(1811..2707)
                     /locus_tag="VGM46_00885"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY4191175.1"
                     /translation="MAENNTVLPGDRTLAYLAIAPSVAIMILLVAVPTVTVFIISFQN
                     VGAGELSGPFVGLRNFERILSDPAFFTALFNSFIWVFGSVALEIVFGLGIAMLLNQQF
                     ALRGLARAIILTPYLIPTVVAVLTWKYMFNDIVGVLNYLLIAAGIIDHPLQWLNSTSM
                     AMFSVILVGVWKFFPFVVIALLGILQAIPQEQYEAAQIDGASALQRFWRITLPHILPV
                     LLLTALLRTIWTFHKFDIIYLMTGGGPINSTTTLPVFVYDEAFVDFTMGRAAAVAIIA
                     FFILSILLAIYLFFMKRAEARQ"
     gene            complement(2700..3752)
                     /locus_tag="VGM46_00890"
     CDS             complement(2700..3752)
                     /locus_tag="VGM46_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4191176.1"
                     /translation="MAKVEIRRVSKSFGEYRAVNEVNLTAEDGKILVLLGPSGCGKTT
                     LLRMIAGLELPTSGDIVIGDKTATRLHPKDRNIAMVFQNYALYPHLTIYENIAFPLRV
                     RRFDGNIDEKVKWAASLVGIENLLARKPRQMSGGERQRTALARSLVRDPQVFLLDEPL
                     SNLDAKLRNSAREELRQFQERIGVTSIYVTHDQVEAMGLGDRICVMQGGVIRQVGTPQ
                     EIYLDPADIFVASFIGSPPMNLVKEEKRTVGFRPEIFLPESCFEAGEDLFRVQFTIQR
                     VEYLGGDRILYGMAGNPLPPTKVVSRLPATVNMSPEIGSTVSFALRQTDLRYFDAEGV
                     AMPVTQRAAVRKVANG"
     gene            complement(4028..5374)
                     /locus_tag="VGM46_00895"
     CDS             complement(4028..5374)
                     /locus_tag="VGM46_00895"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEY4191177.1"
                     /translation="MSNFRMRVRIRSLAMAISVGAMLSAAPAMAKDVVRFLHNETDPP
                     SIEFYNKAIAAFEAENPDIEIQMEAVGTDARLQKVVAALSAKTMPEIFKILPEEIPDF
                     AKKGYIENLDSFIDDIGRDDFVKGSVATIDGKAFSVPYTLGNFSVLFYRDDLLKAKNI
                     EVPKNWDDLKSAAAKLTGDGKYGFIFPAGKNRMTSIFLSALMWSAGGTYFDKDLNVTF
                     DNPGTIKALQFLKDMVPYSPPGIASYSYNDMINAYMTGTVALDIYASRLPANAAANTP
                     DLFANTKPAPMPAGPAGLAVEYVSPDSFALTTPAAGAKSPEAARKFLKYIITGSRLKD
                     FSLTAFPHLIAPLKSVQPDLIKEGSAMLNNRTEFGEQAFSMPNSLDFDTEAGATFEGG
                     KVTMSGIRNPYIGPIVARDIPAVVVQRVVLQGEDPAAAAAWGAEQMKQLVEDAKSK"
     gene            5674..6654
                     /gene="denD"
                     /locus_tag="VGM46_00900"
     CDS             5674..6654
                     /gene="denD"
                     /locus_tag="VGM46_00900"
                     /EC_number="1.1.1.410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018117261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-erythronate dehydrogenase"
                     /protein_id="HEY4191178.1"
                     /translation="MHVMIIGAAGMIGRKLAARLAKSAAIGSSTVDQMTLVDVIPSPA
                     PSAEFGADIRVADLSMPGEAERLIASRPDTIFHLAAIVSGEAEADFEKGYRVNLDGTR
                     LLFEAIRKIGRGQAYRPRVVFSSSIAAFGGSFPDEIEDDFITTPATSYGTQKVIGELL
                     LADYSRRNLLDGIGIRLPTICIRPGKPNKAASSFFSGILREPLIGEEAVLPVSEELRH
                     WFASPRAAIAFLEHAATIDLSALGVRRSLTMPGLSVSVGEQIEALRQFAGDKAVRLIR
                     REPDEAIARIVAGWPSKFNARRAEDLGFRADASFADIVRAHIEDELGGRV"
     gene            6662..7918
                     /gene="otnK"
                     /locus_tag="VGM46_00905"
     CDS             6662..7918
                     /gene="otnK"
                     /locus_tag="VGM46_00905"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007815504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HEY4191179.1"
                     /translation="MLLGCIADDFTGASDLANSLAKEGMRTVQFVGTPRGPVDAPCDA
                     GVIALKTRSIEPAEAVRQSMAALDWLRRQGCRQFLFKYCSTFDSTPQGNIGPVAEALM
                     DALNAPAAVVCPAFPATGRTLFMGHLFVKDRLLNESGMQDHPLTPMTDADIPRWLRRQ
                     TKGNVGLVPYQTVMQGPVAIRDAIAKEAAAGRRLVVVDAISEKDLRVIGAAIRDHPLV
                     TGGSGIALGLPQNFREQALLSSAKESFSPAPGPGVILSGSCSSATRAQVASHLRSHPG
                     FKVDADSLITGRVSVADALTFIKERLNDMPMIYSTADPDTIAATQSLHGREVASRAVE
                     EFFAEMARQMVAVGVTRIVVGGGETAGSVVTALGISHFNIGEEIDPGVPALEAKGVAP
                     LRLALKSGNFGGTDFYARALRALETA"
     gene            7915..8553
                     /locus_tag="VGM46_00910"
     CDS             7915..8553
                     /locus_tag="VGM46_00910"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018067194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HEY4191180.1"
                     /translation="MNQETELRASIVKWGRSIFERGLTSGSSGNISVRLDDRFLMTPT
                     NSCLGFLEADKMSLLDSNGTLLSGDKPTKELPLHFAFYRSRPAAKAVVHLHSTYATAL
                     SCLEDVDCNDAIPAITPYVVMRVGNVPVLPYTKPGAKDAEPMIFAKAADHAAILLGNH
                     GPVVAGRSLEDAVFIMEELEEAAKLIMITRGMNVRLLNQTHVNELNRAYPLK"
     gene            8855..9184
                     /locus_tag="VGM46_00915"
     CDS             8855..9184
                     /locus_tag="VGM46_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191181.1"
                     /translation="MSTTGSTAVSDGDRIAPADIAAALTRAPFVYADGATQVFTPDGH
                     TVYTEHGRPSSGGWKVDDEGRFESFWPPSEYTIYQLSWIAGTDGKAVGIRFTDPNRGA
                     KFDGRYA"
     gene            complement(9258..9719)
                     /locus_tag="VGM46_00920"
     CDS             complement(9258..9719)
                     /locus_tag="VGM46_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HEY4191182.1"
                     /translation="MLLWRLSGREHAGAFDGGYGLHFDGRWNTIGHAVTYCATSPALC
                     VLEKLVHIEDPALMPPLVMVTYEAPDAIGVEEVALDALEPDWRRHEAWSQQRGDAWHE
                     AGATTLLRVPSAIVPVAGSPDLNVLINHNHPAVADIRIVARENFSLDPRLF"
     gene            complement(9719..10156)
                     /locus_tag="VGM46_00925"
     CDS             complement(9719..10156)
                     /locus_tag="VGM46_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011512140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin Xre/MbcA/ParS toxin-binding
                     domain-containing protein"
                     /protein_id="HEY4191183.1"
                     /translation="MSIALVEEVARKLGGQRVLGAIVRSQADLALAVRNRLPLSALRG
                     LSMAGMSEQEIERFVIPQRTRRHRADKQQPLTVDESDRAVRLLRVQSLAETTFGERDK
                     ANRWLRRPLTELGGETPLVVAQTEAGARVIETILGKIAWGAAA"
     gene            complement(10416..12077)
                     /locus_tag="VGM46_00930"
     CDS             complement(10416..12077)
                     /locus_tag="VGM46_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018234787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEY4191184.1"
                     /translation="MTTENSGNALSRTVDRDWWRGAVIYQIYPRSYQDSDGDGIGDLQ
                     GIIRRLPYISALGVDAIWISPFFKSPMKDFGYDVSDYCDVDPMFGTLADFDELIAQAH
                     RLGLKVMIDEVLSHTADAHPWFRESRSSRSNSKADWYVWADARPDGTPPNNWLSIFGG
                     SAWQWDTRREQYYLHNFLAEQPDLNFHNRAVQDALLDVTRFWLERGVDGFRLDTINFY
                     FHSQGLEDNPALPVSERNDQTAPSVNPYNYQDHLYDKSRPENLAFLERFRALLDEYPA
                     AAAVGEVGDSQRGLEVVAAYTAGGKRVHMCYAFDFLAPEKINSVKVREVLESFTRVAS
                     DGWACWAFSNHDVMRHASRWAAGEADMNAYLKVISALLLSLRGSVCLYQGEELGLGEA
                     DLAYEDLRDPYGIRFWPEFKGRDGCRTPMVWDVAATNGGFSDARPWLPVPAEHLAKAV
                     GAQQGDHTSLLEHYRRFLTFRRSHPVLVKGDIEFMDSPEGTVLFTRRTGNDQMLCAFN
                     LGVSAATVDLNATGPFQPLTGHGFGGQQVGTKISLGSYQAWFAKI"
     gene            12289..13350
                     /locus_tag="VGM46_00935"
     CDS             12289..13350
                     /locus_tag="VGM46_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY4191185.1"
                     /translation="MKIAHVAPLYESVPPKLYGGTERVVSYLTEGLVKLGHDVTLFAS
                     GDTRTSARLVACRERALRLDPRPLKSEIAAHLAMLDEVRSRAADFDVIHFHLSHFLHF
                     SFFEDMAQRTVTTPHGRLDYVDLAPAYARFPRFPMISISRNQRRGLPDANWLATIHHG
                     LPVDLYQPNFQPDAQDPYLVFLGRFSRDKRPDRAVEIARRSGLKIKLAAKIGDDDRAY
                     FREVVEPLIDGDRVEYVGEIEEHQKAEFLGRAAGLLFPIDWPEPFGLAVIEAMACGTP
                     VIVWNRGAMPEIVEHGKTGFVVDTVDDAVDMVPALISLDRREVRAAFEARFSAARMAR
                     DYVAAYARLLGMQPEAKAS"
     gene            13347..15527
                     /locus_tag="VGM46_00940"
     CDS             13347..15527
                     /locus_tag="VGM46_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEY4191186.1"
                     /translation="MTERVTSLPLDERTLDPAIALAALDETAPREPHRLFALKHGDCF
                     AVADAYGDIRGIGDGFFRDDTRMLSEFRLTIGGRSTSLLGASLSQDNVLFTSNLTNLP
                     IHSAVGRDIPQGGIHIERVRLIWEDRLFERITLSNYSREHSTFLLSLHFGADFRDMFE
                     VRGSTRPKRGTSHAAETDESSVVLRYEGLDKLVRTSVISFSQTPDHLVADRADFFIAV
                     TKRSHKTFYVEVGAELAQTPDRERFRAAAARARFAMRAKRRHGATVHSSGRVFNDWVA
                     RARADVALLTTELATGPYPYAGIPWFSTAFGRDGVISALQMLWLNPGLARGVLAFLAQ
                     HQATETSLFSDSEPGKIMHETRKGEMVALSELPFGRYYGGVDTTPLYIHLACAYADRT
                     GDMAFIDSLWPSLCAAAEWTEKASRRTGFLTYQRAAESGLANQGWKDSFDSVFHADGR
                     IPKGPIALVEVQGYVFSAFKGLGHLARRRGDDTAADHWEKRAEEMREAVERTFWIEEL
                     GTYALALDGNGEPCEVRTSNAGHLLYMGLPSPQRAQKVADQLMSASFHTGWGLRTLAD
                     DAIFFNPMSYHNGSIWPHDTALCTAGLARYGVRDHVVRLMSGTFEAAVHFNMRLPELF
                     CGFTRAPGEAPIAYPVACLPQAWSAGAAFMLMQSCLGLEIDGWRGEIHVTRPRLPIGI
                     DSLTVRHLQVGSAAVDLTFQRVGDRVGAFLADRHEGLAPLIVKT"
     gene            15653..>16723
                     /locus_tag="VGM46_00945"
     CDS             15653..>16723
                     /locus_tag="VGM46_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEY4191187.1"
                     /translation="MTANGSFAFSSFPVIWALVAASFALSLLAVIFSMRKARHGRASP
                     AAASLDAAGEPIRAVMRESHKNGHSADAALVNWAPDTLRKILADELPGAQVIVVSNRE
                     PYIHNRSEDGVELVVPASGLVSALEPITRSCAGTWIAYGGGTADRLTVDENDRIMVPP
                     NNPSYTLRRVWLTKEEHQGYYLGFANEGLWPLCHIAFTRPIFRASDWETYEAVNRKFA
                     DTVVAEARNERPIVLVQDYHFALLPRMIRERLPEAIVITFWHIPWPNSEVFSICPWRE
                     KILDGLLGSSIIGFHTQFHCNNFIESVDRFLESRIEREDSAISYGGETSLVHAYPISI
                     EWPPDLLATLPAVAQCREQVRRK"
BASE COUNT         3217 a         5272 c         5076 g         3158 t
ORIGIN      
        1 atatggcgct ttgggacata cgcggcaagg ctgtcggcct accgctctat aagttgcttg
       61 gaggcggcca gaggcccatc aaggcttatg cgggcggcat ttcgcttggc taccaggaac
      121 cggccagtct ggcgcgcgaa gcccatgaca tggtttcgcg gggctatcgg gcgatcaagc
      181 tgcgcatcgg cgatacgccg caacgcgaca ttgcgcgcat atccgaggta cgcaaaagca
      241 tcggtgacga aatcgacatt ctcaccgacg ccaacgccaa cgccaccatg gagcatgtga
      301 gagccatcat gcccagcctc gacgaaaacc gtgtcggctg gcttgaggaa ccattcgccg
      361 cgcatgatta caggtcgtac cgccaggcga cgcgatttgg cacgacgccc ttggccgccg
      421 gcgaaaatca ttacacgcgc ttcgagtttc accggctggt cgaggacggc gtgatcacct
      481 atctccagcc cgacctgtcc aaggccggcg gcatcaccga ggcgctgcgc attgcggcga
      541 tggcttctgc ttggaaactg tcgatcaatc cccattgctc ggcgaccggg ttgaacatgg
      601 cggcgacgct ccattttctc gcatcgatcg acaatccagg ctatttcgaa gccgaggcgg
      661 caagcttcaa tcccttccgc gacgatcttt gcagcatgag ctacaaggtg ggcgccgatg
      721 ggttcgtaca cgttcccgaa ggccccggca tcggcatcga agtcgacgag gcattcattg
      781 cccgccaccc gcttatcgat ggtcccggct atgtgaagta gcctcgtatt tccggcaaaa
      841 aagccgcgct cccgccgggg aacgcggctt tttcgtggag acgaagcgtt acctgcgcag
      901 gcttattcgc ccttcacgcc gcccgccgaa agaccggtta tcaaattgcg ctgcacaagc
      961 aggtagaaga ccacgaccgg gatcgtcacc agcaccgcgc cggccagcag catgccccag
     1021 gaatagatgt tgtcctggcc tatccaggtg gccaggccca gcgacaggac cttgttggtc
     1081 tcggaggatg tgaacaccag agcgaacagg aactcgttcc aggcgtgatt gaacgtgaac
     1141 accccaaccg acaccagccc cggcaaggcc aatggcaagg taacccggcg catcgcctgg
     1201 aaacgcgtgc agccgtctat catcgcgctt tcctccaggc ttgccgggat cgcgttgaaa
     1261 taggaccgca tgaaccacaa cgtaagcggc aaggtgaagg ccgtgttggc gatgatgagc
     1321 gaaagcagcg tgtccaccag cccgagcttg acgacataga tgtagagcgg aatgaccagc
     1381 aggacgtctg gcagcatgta gcagatcagg gtcagcagcg aaaacgtgcc tagcccgaag
     1441 aagcgaaacc ggctgaggct ataggcgcca aggccgccga tgacgaggga aagcgcggta
     1501 gcaccccccg ccacgatcag gctgttcatg aagtagatcg ggaaattcgt ttgaacgaac
     1561 aggtcgcggt aattggtgaa cgtccactga tgcggaagaa acgccggcgg gaactgatag
     1621 agctcgcccg aggtcttgaa cgagcagatg accatccaga acagcggaaa acctacgacg
     1681 caggtgaccg tgatgaccag caggtagatc atgagccggg acgtgacgtg gtggagatcg
     1741 gccatatcca tattgcggcg gacaggcggg cgggcgcgcc cggcagcgcc aaggctgatt
     1801 tgtgaggcgg tcattggcgt gcctccgcgc gcttcatgaa gaaaagatag atcgcaagca
     1861 ggatcgagag aatgaagaag gcgatgatgg cgacggcggc ggcgcgcccc atggtgaaat
     1921 ccacaaaggc ctcgtcatag acgaagacgg gaagcgtcgt cgtcgagttg attggcccgc
     1981 cgccggtcat caggtagatg atatcgaatt tgtggaaggt ccagatcgta cgcaggagag
     2041 ccgtcagcag cagcaccggc aggatgtgcg gcaaggtaat tcgccagaac cgctgaaggg
     2101 cgctggcccc gtcgatctgc gccgcttcat attgctcctg cgggatggct tgcaggatgc
     2161 cgagaagggc aatcacgacg aaggggaaga acttccacac gccgaccaga atgaccgaga
     2221 acatcgccat cgaggtgctg ttcagccact gaagcggatg atcgattatt ccggcggcta
     2281 tcaacaggta gttcaatacg ccaacgatgt cgttgaacat gtatttccag gtaagcaccg
     2341 caacgaccgt ggggatgagg tacggggtaa ggatgatcgc gcgggcaaga ccgcgaaggg
     2401 cgaactgctg gttcaagagc atggcaatgc caagaccgaa tacgatctcc agcgcaacgc
     2461 tgccgaacac ccagatgaac gaattgaaaa gggcggtgaa gaatgccgga tcgctcaaga
     2521 tgcgctcgaa attgcgaagg ccgacgaagg ggccgctcag ttcgccggcc ccgacatttt
     2581 gaaagctgat gatgaaaacg gtcaccgtcg gcacggcaac aagaaggatc atgatcgcca
     2641 cgctcggcgc tatcgccaga taagcgagcg ttctatctcc gggaaggacg gtgttgttct
     2701 cagccatttg ctactttccg tacggccgcg cgttgtgtca caggcattgc aaccccttcc
     2761 gcatcgaagt agcgcagatc cgtctggcga agggcgaagc tgacggtcga gcctatttcg
     2821 ggcgacatgt ttacggtggc cgggagcctc gaaaccacct ttgtcggcgg caggggattg
     2881 ccggccattc cataaagaat gcggtcgccg ccaagatatt cgacgcgctg gatggtgaac
     2941 tggacgcgga acaaatcttc gccggcttcg aagcagcttt ccggcaggaa gatttccggc
     3001 cggaagccga ccgtgcgctt ttcttccttg acgaggttca tgggcggcga gccgataaaa
     3061 ctggccacga aaatatcggc gggatcgaga tagatttcct gcggcgtgcc gacctgccgg
     3121 atcaccccgc cctgcatcac gcagatgcgg tcgccaagcc ccatcgcctc cacctggtcg
     3181 tgcgtcacat atatggatgt tacgccgatg cgctcctgga actggcgcaa ttcctcgcgc
     3241 gccgagttgc ggagcttggc atcgaggttt gaaagcggct cgtcgagcag gaacacttgg
     3301 gggtcgcgca cgagggacct ggccagcgcc gttcgctggc gttcgccgcc cgacatctgg
     3361 cggggcttgc gggcaagcag attttcgatg ccgaccagtg aagccgccca ctttactttt
     3421 tcgtcgatat ttccgtcgaa acggcgcacc cgcagcggaa acgcgatgtt ttcgtagatc
     3481 gtcaggtgcg gatagagcgc ataattctgg aataccatcg caatattgcg atctttcgga
     3541 tgcagcctcg tggcggtttt atcgccaatc acgatatcgc ccgacgtggg cagttcaagg
     3601 ccggcaatca tgcgcagcag cgtggtcttg ccgcaacccg acggccccag cagcaccaga
     3661 atcttgccgt cctccgccgt cagattgacc tcgttgacgg cacgatattc accgaaggat
     3721 ttgctgacgc gcctgatttc aactttggcc atgaattata tcctgcttcc gatattgttg
     3781 ggtttggccc ctgccggatt tccgaccttc gcgacgagat gttccagccg cccgattgat
     3841 ggcgcggtcg ccaccggcgg gcaactctgc ctgccggtac tctttcgacg gccatcaacc
     3901 gtgcgttgca gccgacatcg aggcaggcgg acactttcgg ccctcgtcgc cggggcagtc
     3961 aagcaagaga gatcggctgt cccgcgctgc ccagacacct ctatccgggc agcgcgggac
     4021 atattcgtca cttgcttttc gcatcctcga cgagctgctt catctgctcg gcgccccagg
     4081 cggcggcggc ggccgggtcc tcgccctgaa gaaccacgcg ctgtacgacc acggcgggaa
     4141 tgtcgcgggc gacgatgggg ccgatatagg ggttgcggat gccggacatc gtcaccttgc
     4201 caccttcaaa ggtcgcgccg gcctccgtat cgaaatcgag cgaattcggc atcgaaaatg
     4261 cctgctcgcc gaactcggta cggttgttca gcattgccga gccttccttg atgaggtccg
     4321 gctgcacgct cttcaacggc gcgatcaagt gggggaacgc ggtcagtgaa aagtccttca
     4381 gccggctgcc cgtgatgatg tatttgagga acttgcgcgc cgcttcgggg ctcttggcgc
     4441 cggcggcggg cgttgtcagg gcgaagctgt cggggctgac atattcgacc gccaatcccg
     4501 caggtccggc cggcatcggc gccggcttgg tgttggcaaa cagatcgggc gtattggccg
     4561 ccgcgttggc cggcaggcgg gatgcgtaaa tgtcgagcgc aaccgtgccg gtcatgtagg
     4621 cgttgatcat gtcattatag gaataggacg cgatgccagg cggcgaataa ggcaccatgt
     4681 ccttgaggaa ttgcagcgcc ttgatcgttc cgggattgtc gaaagtgacg ttgaggtcct
     4741 tatcgaaata ggttccgcca gccgaccaca tgagagcgct caggaaaatg ctggtcatcc
     4801 ggttcttgcc ggctggaaag atgaagccgt actttccgtc gcccgtgagc ttggcggcgg
     4861 cgcttttgag gtcatcccaa tttttcggga cctcgatatt cttggctttc aacaggtcgt
     4921 cccgatagaa caggacgctg aaattgccga gcgtataggg aacgctgaac gccttgccgt
     4981 cgatcgtggc gacggaccct ttgacgaaat cgtcgcgacc gatatcgtct atgaagctgt
     5041 cgaggttctc gatgtatccc ttcttggcga aatccgggat ttcctccggc aatatcttga
     5101 agatttcggg catggtcttc gccgacagcg ccgccacgac cttctgcaag cgcgcgtcgg
     5161 tcccgaccgc ttccatctgt atttcgatat ctggattctc ggcttcgaac gcggctatcg
     5221 ccttgttata gaattcgatg ctggggggat ccgtttcatt gtgcagaaac cggaccacgt
     5281 ctttggccat cgccggcgcc gcactcagca tcgcgccgac cgaaatggcc atggccaggc
     5341 tcctgatacg aacccgcatt ctaaaattac tcacgacaat cctcccgttc attgatagcg
     5401 gcgttgttgg cgcttgttca tcctccgcga ccgctgggtc ggaagagacc cgtttcattc
     5461 catcacaacg gccgatatcc aacattttgc ttgtaacata attcatcgaa gtgtaaacat
     5521 aaattcacaa gatggtttca aatcctaaca ttcatgaact ttcgtacgaa attctgcgtg
     5581 cggctcggtt tgccggcgcg tgggttttct gaaccgaaag caagaccatg gctgagcgac
     5641 cagcagccaa cgggaagcgc cagagagaac cgaatgcatg tgatgattat tggcgcggcc
     5701 ggaatgatcg gccgcaagct cgctgcccgg ctggccaaaa gcgctgcaat cggtagttcg
     5761 accgtcgatc agatgacgct cgtcgatgtc atcccctcgc ctgctccttc ggccgaattc
     5821 ggcgccgaca tccgcgtcgc cgatctgtcg atgccgggcg aggccgagcg gctcatcgct
     5881 tccaggccgg acacgatttt tcacctggcg gccattgttt caggggaggc cgaggcggat
     5941 ttcgagaaag gctaccgcgt caatctcgat ggaacgcgac ttttgttcga ggcaatccgg
     6001 aaaatcggca ggggccaggc ctaccgtcca cgcgttgtgt tttcatcgtc catcgcggcg
     6061 ttcggagggt cgttccccga cgagatagag gacgacttca tcaccacgcc cgccacaagc
     6121 tacggtacgc aaaaggtgat cggcgaattg ctgctggccg actatagccg gcgcaacctt
     6181 ctggacggca tcgggattcg cctgccgacg atctgcatac ggcccggaaa acccaacaag
     6241 gccgcttcaa gtttcttttc cggcattctt cgtgaaccgc tgatcgggga ggaagccgtg
     6301 ctgccggttt cggaggaatt gcgccattgg ttcgccagcc cccgtgccgc cattgccttc
     6361 cttgaacacg ccgccacgat cgacttgtcg gcgcttgggg tacgccgcag cctgaccatg
     6421 cctggattgt cggtgtcggt gggtgagcag atcgaagcct tgcgacaatt cgcgggcgat
     6481 aaggccgtcc ggctgatccg gcgcgaaccg gatgaggcca tagcgcgcat cgttgcggga
     6541 tggccaagca aattcaatgc acggcgcgcc gaagaccttg gctttcgggc ggatgcctcc
     6601 tttgccgata tcgttcgcgc acatatcgaa gacgaactcg gcggccgggt gtgaggacca
     6661 gatgctgctt ggatgcatag ccgacgattt caccggcgcc agcgaccttg ccaactcgct
     6721 ggccaaggaa ggaatgcgca ccgttcagtt cgtcggcacg ccgcgcggac cggtggacgc
     6781 gccatgtgac gccggcgtca tcgccctcaa gacccgctcg atagagccgg ccgaggcggt
     6841 gcgccaatcg atggccgcgc tcgactggct tcgccggcag ggatgccgcc agttcctgtt
     6901 caaatactgc tccactttcg attcgacccc gcaaggcaat atcggtcccg tcgccgaggc
     6961 gcttatggat gcgttgaacg cgccggccgc cgtcgtttgc ccggctttcc ccgcaacggg
     7021 ccgaacgctg ttcatggggc atttgttcgt caaggatcgc ctgctcaacg agtcgggcat
     7081 gcaggaccat cccctgacgc cgatgacgga tgccgatatc ccgcgctggc tgcgccgcca
     7141 gacaaagggc aatgtcgggc tggtgcctta ccagaccgtt atgcagggac cagttgctat
     7201 ccgcgacgcc atcgccaaag aagctgccgc aggccggcgc ctcgtcgtgg tcgacgcgat
     7261 atcggaaaag gacctgcggg tgatcggcgc cgcgatccgc gatcatccgc ttgtcaccgg
     7321 aggctccggc atcgcgcttg gccttccaca gaactttcgg gagcaagcgc ttctttcctc
     7381 cgcgaaggaa agcttttccc cggcaccggg accgggcgtc atcctctccg gctcgtgctc
     7441 atcggcgaca cgggcgcagg tcgcctcgca ccttcggtcg catcccggct tcaaggtaga
     7501 cgcagacagt cttatcaccg gccgggtgag cgttgccgac gcgctgacct tcatcaagga
     7561 acggctgaac gacatgccga tgatctattc gacggcagac cccgacacca tcgccgctac
     7621 gcagtccctt catgggcgcg aggtcgcctc gcgggccgtg gaggaatttt ttgccgaaat
     7681 ggcaaggcag atggttgccg tcggcgtcac ccgcatcgtt gtcggcgggg gcgaaaccgc
     7741 cggctcggtt gtgaccgccc tgggcatatc gcacttcaat atcggagaag agatcgaccc
     7801 cggcgtgccg gcgcttgagg ccaagggggt agcgccgcta cgccttgctc tcaagagcgg
     7861 gaatttcggc ggtactgatt tttacgcccg agcgttacgc gcactggaga cggcatgaac
     7921 caggaaaccg aactcagggc ttccatcgtc aagtgggggc gttcgatctt cgagcgaggc
     7981 ttgacgtcgg gttcgtcggg caacatctcg gtcaggctcg acgacaggtt cctgatgacc
     8041 cccacgaatt cgtgcctcgg cttcctggag gccgacaaga tgtcgctgct tgattccaac
     8101 ggaaccctgc tgtcgggcga caaaccgacg aaagaactgc ccttgcattt cgcgttctat
     8161 cgaagccgcc ctgccgcgaa ggctgtcgtg catctgcatt ccacctatgc gacggccctt
     8221 tcgtgcctgg aagacgtgga ctgcaacgat gcgatcccgg caatcacgcc ctatgtggtg
     8281 atgcgcgtcg gcaacgtacc ggtcttgccc tacacgaagc cgggagcgaa ggatgccgaa
     8341 ccgatgatct ttgccaaggc ggccgatcac gccgctatcc ttctgggcaa tcacggtccg
     8401 gttgtggcgg gacgatcact tgaggatgcc gtcttcatca tggaagagtt ggaggaggcc
     8461 gcgaaactca tcatgatcac gcgcggcatg aatgtccgcc tgctcaatca aacccatgtc
     8521 aacgagctca atcgggccta cccgttgaaa tagcgaaagc aggcccggcg ccggcgacag
     8581 gccggcttgc gaagcttgtc ctccgcgagc cacgcgcttc gaattcgccg gcctcgtgcc
     8641 gttcatgaag ctggcccgac gcggcgtcgg ccgtgccggt tgacctttcg gtcgcgttac
     8701 cagccgttat gaaaacgata cctgccgcta aattgaatag gcgaaccgat cctgcaaaag
     8761 tgaccacctc gcctgaccaa ttccgtcgtg ccacctgtgc ttggcatccg gcttggtggg
     8821 cgacgcccga cacacacctc gaaaggagga gaagatgtcc actacaggat caacggctgt
     8881 cagcgacggc gaccgtatcg ccccggccga catcgccgcc gcactgacgc gagcaccctt
     8941 tgtctacgcc gacggcgcga cgcaggtgtt cactcccgac gggcacaccg tctacaccga
     9001 gcacggcagg ccgtcgtccg ggggatggaa agtagacgat gaggggcgct tcgagtcgtt
     9061 ctggccgcca tcggaataca cgatctacca gctttcctgg atcgccggta ctgacggcaa
     9121 ggccgtcggg atccggttca ccgatccaaa tcgaggggca aaattcgacg ggcgatacgc
     9181 atagggcgct cgcctagcct ggtttcgcat tggctgccca agacggtcat cgctttgcca
     9241 tgaccaaccc ccgcggctca aaacagccgc gggtcgagcg aaaaattctc ccgcgcaacg
     9301 atgcggatat ccgctacggc cgggtgatta tggttgatca gcacgttcag gtccggcgag
     9361 ccggcgacag gcacgatggc tgaaggcacg cgcagcaacg ttgtcgcgcc tgcctcatgc
     9421 caggcgtcgc cccgctgctg gctccaggcc tcatgtcttc gccagtcagg ctccagcgca
     9481 tcgagcgcca cttcctcgac gccgatggca tccggcgcct cataggtcac catcaccaat
     9541 ggcggcataa gggccgggtc ttcgatgtgg acaagctttt ccagcacgca caaggctggc
     9601 gaggtcgcgc aataggtgac cgcgtgaccg atcgtgttcc agcggccgtc gaaatgcagg
     9661 ccgtagccgc cgtcgaaggc ccccgcatgc tccctgcccg acaaccgcca aagcagcatc
     9721 aggcagccgc accccaggcg atcttgccaa gaatcgtttc gatgacgcgc gcgccggcct
     9781 cggtctgcgc caccaccagc ggcgtctcgc cgccaagctc ggtaagcggg cgccgaagcc
     9841 agcgattggc cttgtcgcgc tcgccgaacg tggtctcggc aagcgattgc acccgcagga
     9901 gccgtacggc gcggtccgac tcgtcgacgg tcaacggctg ctgcttgtcc gcgcgatggc
     9961 ggcgcgtgcg ctgcggaata acgaagcgct cgatctcctg ctcgctcatg cccgccatac
    10021 tcaagccacg cagcgccgac aacggcagcc gattgcgcac cgcaagcgcc agatcggcct
    10081 gcgaccgcac gatcgcgccc agcacccttt ggccgccgag cttgcgggcg acttcctcta
    10141 cgagcgcaat cgacatgcct gtctcctgat atgaatggca acttgccatc tatatatacg
    10201 gcacattgcc gacttacaag gctaccaggc ccagcggtgc caagccgggt ggtgctaagc
    10261 aaggtggtgc cagtccagac cgcctatgtg gaatgtcttc atttcaaaat attcctcaat
    10321 gtcggcctgc gcttcttcat tgcggcccct agcccgaggc agattcggac ccttcgacgg
    10381 cattggccgt cagggttcga tactgcattt cccactcaaa tctttgcaaa ccaggcttga
    10441 tagctgccga ggctgatttt cgttccaacc tgttggccgc caaatccgtg tccggtcagg
    10501 ggctgaaacg ggccggtggc gttgaggtcc acggtggcag ccgagacgcc gaggttgaaa
    10561 gcgcatagca tctggtcgtt gcccgttcgg cgcgtgaaca ggacggttcc ctccggtgaa
    10621 tccataaatt cgatatcgcc cttcaccagc acaggatgtg agcggcggaa ggtgaggaaa
    10681 cgccggtagt gctcaagcag cgaggtgtgg tcgccctgct gcgcgccaac tgccttcgcc
    10741 agatgttcgg ccggcaccgg cagccatggc ctggcgtctg aaaagccgcc atttgttgcc
    10801 gccacatccc acaccatcgg cgtgcggcag ccgtcgcggc ccttgaattc cggccagaaa
    10861 cggatgccgt acgggtcgcg caaatcctca taggcaagat cggcctcgcc aagcccaagt
    10921 tcctcgccct gatagaggca gaccgagccg cgcagcgaca aaagcagcgc cgagatcacc
    10981 ttcagatagg cgttcatgtc cgcttcgccc gccgcccagc gcgaggcgtg gcgcatcaca
    11041 tcgtggttgg agaaggccca gcatgcccag ccgtcgctgg cgacgcgagt gaagctttcg
    11101 agcacctcac gcaccttgac ggaatttatc ttttcgggcg ccaggaagtc gaaagcgtag
    11161 cacatatgga cgcgcttgcc gccggcggta taggccgcga ccacctccag cccgcgctgc
    11221 gaatcgccga cctcgccgac cgccgccgct gccggatatt catccagaag cgcgcggaag
    11281 cgttcgagga aggcgaggtt ttcaggccgg ctcttgtcat agagatggtc ctggtaattg
    11341 tacggattga ccgaaggcgc ggtctggtcg ttgcgttcgg aaaccggcag cgccggattg
    11401 tcttcaagac cctgcgaatg gaaatagaag ttgatggtgt cgaggcgaaa gccgtcgacc
    11461 ccgcgctcca gccagaaccg ggtgacgtcc agcagcgcgt cctgcacggc acggttatgg
    11521 aaattcaggt ccggctgctc ggccaagaaa ttgtgcaggt aatattgctc gcgccgcgtg
    11581 tcccactgcc acgccgagcc gccgaagatc gacagccagt tgttgggcgg cgtgccgtcc
    11641 ggcctggcgt cggcccacac ataccaatcg gccttggaat tgctacggct ggagcgactt
    11701 tcccggaacc agggatgcgc atcggcggtg tgcgacaaaa cctcgtcgat catgaccttg
    11761 agaccgaggc ggtgcgcctg cgcgatcaac tcatcgaaat cggcaagcgt cccgaacatc
    11821 ggatcgacat cgcaataatc ggaaacgtca tagccgaaat ccttcatcgg cgacttgaag
    11881 aacggcgata tccaaatggc atcgacgccc agcgccgaaa tatagggcag acgccggatg
    11941 atgccttgca ggtcgccgat gccgtcgccg tcgctgtcct gatagctgcg cggatagatc
    12001 tggtagatca ccgcgccacg ccaccagtcg cggtccacgg ttcgggacaa ggcgtttcca
    12061 gagttttcag tcgtcatgct tttgttcggc tttctgtagc gatgccaccg cgcgccctga
    12121 ccgaaaccgc cgcgccgtga aaatcgttcc gcccggcggg atatttggcc aaggatattt
    12181 gcccttcggc tcatccgcca acgcggcgaa aatgccaaaa acaggaacga tcctccggct
    12241 gaagcatttc ccgactgcac caccgcttga ttgagggaac gcagatcaat gaagattgcc
    12301 catgtcgcgc cgctctacga gtcggttccg cccaagcttt atggcggcac cgagcgtgtc
    12361 gtctcctatc tgacggaagg gctggtcaag cttggccacg acgtgacgct gtttgccagc
    12421 ggcgatacca gaacctccgc caggctggtg gcctgccgcg agcgcgcgct gcggctcgat
    12481 ccgcggccgc ttaaatcgga aatagcggcg catctcgcca tgctggacga ggtgcgaagc
    12541 cgggctgcgg atttcgacgt catccacttt cacctcagcc acttcctgca tttctccttt
    12601 ttcgaagaca tggcccagcg caccgtgacg accccgcatg gaaggctcga ctatgtcgat
    12661 ctggcgcccg cctatgcgcg ctttccccgc tttccgatga tttccatttc cagaaaccag
    12721 cggcgcggac tgcccgacgc caactggctg gcgaccatcc atcacggctt gcccgtcgac
    12781 ctgtaccagc cgaatttcca gccggatgcg caagatccct atctggtctt tctcggccgc
    12841 ttttcgcgcg acaagcggcc cgaccgcgcc gtcgagatcg ccaggcgttc cggcttgaag
    12901 atcaagcttg ccgcgaagat cggcgatgac gaccgcgcct atttccgcga agtggtggag
    12961 ccgttgatcg acggcgaccg cgtcgaatat gtcggcgaga tcgaggagca ccagaaggcg
    13021 gagtttctcg gccgcgcggc gggccttttg ttccccatcg actggcccga accgttcggc
    13081 ctcgccgtca tcgaggccat ggcctgcggc acgccggtca tcgtctggaa ccgtggcgcg
    13141 atgccggaga tcgtcgagca tgggaagacc ggcttcgtcg tggacacggt agatgacgcc
    13201 gttgacatgg tgccggccct catttccctc gacaggcgcg aggtgcgagc cgcgttcgaa
    13261 gcgaggtttt ccgccgccag gatggcgcgc gactatgtgg ccgcctatgc ccggctgctc
    13321 ggcatgcagc cggaggcgaa agcgtcgtga ccgagcgcgt cacgagcctt ccgctggacg
    13381 agcgcacgct cgacccggcc atagcgctgg ccgcgctcga cgaaaccgcg ccgcgcgaac
    13441 cgcaccggct gtttgcgctt aagcatggcg actgctttgc cgtcgccgat gcctatggcg
    13501 acatcagggg cattggcgac ggcttcttcc gcgacgacac gcgcatgctt tccgaattcc
    13561 ggctgaccat cggcggacgc tccacctcgc tgctcggcgc ttcgctgagc caggacaatg
    13621 tgctgttcac gtccaatctg accaatttgc cgatccacag cgccgttggc cgcgacatac
    13681 cgcagggcgg catccacatc gagcgggtgc gcctcatctg ggaagaccgg ctgttcgagc
    13741 gcatcacgct ttccaactac agccgcgaac attccacctt cctgctgtcg ctgcatttcg
    13801 gcgccgattt ccgcgacatg ttcgaagtcc ggggatcgac gcggccgaag cgcggcacct
    13861 cccatgccgc cgagaccgac gaaagctcgg tcgtgcttcg ctatgagggg ctggacaagc
    13921 tcgtgcgcac ctcggtgatc tcgttttcgc agacgccgga tcatctcgtc gccgaccgcg
    13981 ccgatttctt catcgccgtc accaagcgca gccacaagac gttttatgtc gaggtcggcg
    14041 cggaactggc gcagacgccc gaccgcgagc gcttccgggc cgccgccgcc cgcgcccgat
    14101 tcgcgatgcg cgccaagcgc cgccatggcg ccaccgtgca cagttccggc cgcgtcttca
    14161 acgactgggt ggcgcgggcg cgtgccgatg tggcgctgct gaccaccgag cttgcgaccg
    14221 ggccttaccc ctatgccggc atcccctggt tttcgaccgc cttcggccgc gacggcgtca
    14281 tctccgcctt gcaaatgctg tggctcaatc ccggcctggc gcgcggcgta ctggctttcc
    14341 tggcgcagca tcaggcaacc gaaacctcgc tgttcagcga ttccgagcct ggcaagatca
    14401 tgcacgaaac ccgcaagggc gagatggtgg cgctgagcga gctgcccttc ggccgctatt
    14461 atggcggcgt cgacaccacg ccgctttata tccacctggc ctgcgcctat gccgaccgca
    14521 ccggcgacat ggcgttcatc gacagcctgt ggccgtcgct ttgcgccgcc gccgaatgga
    14581 cggagaaagc cagccggcgc accggcttcc tcacctacca gcgcgccgcc gaatccggcc
    14641 tcgccaacca gggctggaag gacagtttcg attcggtgtt ccatgccgat ggccgcatcc
    14701 ccaaaggccc gatcgcgctg gtcgaggtgc agggctatgt cttttccgcc ttcaaggggc
    14761 tgggccacct tgcgcggcgg cgcggcgacg acaccgccgc cgaccactgg gaaaagcgcg
    14821 ccgaggaaat gcgcgaggcg gtcgagcgga cattctggat cgaggaactc ggcacctatg
    14881 cgctggcgct cgacggcaat ggcgaaccgt gcgaggtgcg cacctccaat gccggacacc
    14941 tgctctatat gggcctgccg tcgccgcaac gcgcgcagaa ggtggcggac cagcttatgt
    15001 ctgcctcgtt ccataccggc tgggggctgc gcacgctggc cgacgacgcc atcttcttca
    15061 acccgatgtc ctatcacaac ggctcgatct ggccgcacga tactgccctt tgcaccgccg
    15121 gcctcgcgcg ctacggcgtg cgcgaccatg tcgtgcggct gatgagcggc accttcgagg
    15181 cggcggtgca tttcaatatg cggctgccgg aactgttttg cggcttcacc cgcgcgccgg
    15241 gcgaagcgcc catcgcctat ccggtcgcct gcctgccgca ggcatggtcg gcgggcgccg
    15301 ccttcatgct catgcagtcc tgcctcggcc ttgagataga cggctggcgc ggcgaaatcc
    15361 acgttacccg gccacggttg ccgatcggca tcgacagcct gaccgtgcgc catctccagg
    15421 tcggcagcgc cgccgtggac ctgaccttcc agcgcgtcgg cgaccgcgtt ggcgctttcc
    15481 tggccgaccg ccatgagggg ctggcgccgc tgatcgtcaa gacgtgaaaa atcaggaacc
    15541 cttccgccga accgcgcgtt agttgggtgg cgtcgccggc gtggccggca gcgcatccct
    15601 ttatcggcat ggagcaggac cggaccccgg cccgaggaca tttggaattg ccatgacagc
    15661 taacggctcg ttcgccttct cttcattccc ggtcatctgg gcgctggttg cggcaagctt
    15721 cgcattgtcg cttcttgcgg tgatcttttc gatgcgcaag gcgcgccatg gccgggcaag
    15781 ccctgccgcc gccagcctgg atgcggctgg cgagccgata agggcggtga tgcgcgaatc
    15841 ccacaagaac ggccactccg ccgatgcggc gctggtaaac tgggcgcccg atacgctgcg
    15901 caagatcctg gccgacgaat tgcccggcgc gcaggtcatc gtggtttcca accgcgagcc
    15961 ttatatccac aaccgatccg aggacggcgt ggaactggtg gtgccggcca gcggcctcgt
    16021 ctcggcgctg gagcctatca cccgctcctg cgcgggaacc tggatcgcct atggcggcgg
    16081 cacggcggac cgcctgacgg tcgatgaaaa cgaccgcatc atggtgccgc ccaacaatcc
    16141 gtcctatacg ctgcgccgcg tctggctgac caaggaagag caccagggct attatctcgg
    16201 ctttgccaat gagggcttgt ggccgctttg ccatatcgcc ttcacgcggc cgatcttccg
    16261 cgcttccgac tgggaaacct acgaggccgt aaaccgcaag ttcgccgaca cggtggtagc
    16321 cgaagcgcgc aacgaacggc cgatcgtgct ggtgcaggac tatcacttcg cgctgctgcc
    16381 gcgcatgatc cgcgaacgcc tgccggaggc catcgtcatc accttctggc atattccgtg
    16441 gccgaattcg gaggtgttca gcatctgccc atggcgcgag aaaatcctcg acggcctgct
    16501 tggcagttcc atcatcggct tccacacgca gttccactgc aacaacttca tcgaaagcgt
    16561 cgaccgcttc ctcgaaagcc gcatcgagcg cgaggattca gccatttcct atggcggcga
    16621 gaccagcctg gttcatgcct atccgatttc catcgagtgg ccgccggacc ttctcgcaac
    16681 cctgcccgcg gtcgcgcaat gccgcgagca ggtgcgccgc aaa
//
LOCUS       DASYCS010000022         7509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_148918,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000022 DASYCS010000000
VERSION     DASYCS010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 7509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7509
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148918"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="VGM46_00950"
     CDS             <1..768
                     /locus_tag="VGM46_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEY4191188.1"
                     /translation="RAVAMRPYVDRILCILPFEQGELARLGGPPGIYVGHRLSHDAGV
                     LAAAKAQAAPHDFSTEREKTLLILPGSRSGEVNRLIGPFGETVAALRARGHHLRLLLP
                     TVPHVAELVRSSVSGWEQQPEIIVDQERKWQAFGQADAALIASGTVSLELALSRVPMI
                     SCYKLDPLARQLARRVMISWSASLPNLIADQPLVPEYYDDFVRPQILARELEALFADS
                     GMRSWQKEGFAEVARRMATAQPAGELAAEAVLQLVRK"
     gene            complement(814..2103)
                     /gene="gltA"
                     /locus_tag="VGM46_00955"
     CDS             complement(814..2103)
                     /gene="gltA"
                     /locus_tag="VGM46_00955"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEY4191189.1"
                     /translation="MTEKTAKLELEGKSHEFKVRSGSVGPDVIDIASLYSSTGAFTYD
                     PGFTSTAACESAITYIDGDAGILMHRGYPIDQLAEHGDFLEVCYLLLYGELPTKAQKD
                     DFDYRITRHTMVHEQMSKFFTGFRRDAHPMAVMCGVVGALSAFYHDSTDISDPYQRMI
                     ASMRLIAKMPTIAAMAFKYHIGQPFVYPKNDLGFAANFLHMCFAVPCEEYKVNPVLAR
                     AMERIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGPAHGGANEAALNML
                     GQIGHVDHIPEFITRAKDKNDPFRLMGFGHRVYKNYDPRAKIMQKTAHEVLGELGIKD
                     DPLLDIAMELEKIALTDEYFIEKKLYPNVDFYSGITLKALGFPTTMFTVLFAVARTVG
                     WISQWKEMIEDPLQKIGRPRQLYTGSPKRDYVPIAKR"
     gene            complement(2280..3704)
                     /gene="gltX"
                     /locus_tag="VGM46_00960"
     CDS             complement(2280..3704)
                     /gene="gltX"
                     /locus_tag="VGM46_00960"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEY4191190.1"
                     /translation="MSDKVVTRFAPSPTGFLHIGGARTALFNWLYSRHLGGTMLLRIE
                     DTDRERSTDAATAAILDGLSWLGLEWNGETVSQFERAPRHREVAEELVRLGKAYYCYA
                     SPAELTEMRDRARAEGRPPRYDGRWRDRDPSEAPAGVAGAIRIKAPTEGETVVHDLVQ
                     GEVRFPNKDLDDFIILRSDGNPTYMHAVVVDDHDMDVTHIIRGDDHLTNAARQTVIYN
                     AMGWAVPHMAHIPLIHGADGAKLSKRHGALGVEAYRAMGYLPEALLNYLARLGWSHGD
                     DEIMSIADMIKWFDITDVNKGAARFDFAKLEALNGVHMRRMDDAELMAIFVETLPYLE
                     GGPAIAARLDAGRKAQLLAALPGLKERAKTLVELADGAGYLFAERPLPIDEKAAALLG
                     GEAKAILSGAHAALGDFSGEWNAANAEAAIREFATTAGHKLGAVAQPLRAALTGKSTS
                     PGVFDVLAVLGRDESLSRIGDQID"
     gene            3802..6393
                     /locus_tag="VGM46_00965"
     CDS             3802..6393
                     /locus_tag="VGM46_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008875900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEY4191191.1"
                     /translation="MAGAGRGGGEGAGAIKSAGERELFSLDPDIQGSPYPPPRLPQTQ
                     PPLSPERDSAPEALPAKGGRDSASHGLPTKGRRDSAPDGFPTRGWRAGKRLRAISVGE
                     LPRLALVAAATELDRGAAFLLVPVFLAVGVVFYFSLSAEPGLLQPAAVVGLLAGLAFL
                     SRHRPVAYLALVAAMLCALGFVTAKLETLRADTQMLGSEISTRLTGRVVAVDAMETGR
                     MRVTLDVISTARPTLRYIPARVRVSAREIPPGTQAGSVLVGFARLLPPTGPVRPDSYD
                     FSFQSYFDGIGGSGFFLTNPKLAALQPIPLSARINAAIENAREAIAGHIRDSIGGAEG
                     EIAAALIVGVRAGIPDAINEAMRKTGIYHIISISGLHMALVAGTIMAMLRGGFALFPE
                     FSSRRPVKKYAAAAALFSIAAYLLISGVVVAAERSFIMLAVMLVAVLFDRAALTMRNL
                     AISAIAVIVVSPHEVVGPSFQMSFAATAALVGAYAGWSDYRATKAGGPPPPRRSWPAF
                     LTRKFFGGMGGLALTSIIAGCATTLFAMWHFQRVAPLSLFANLAVMPIVSVVVMPFAV
                     LSALAMPFGADGPLLYVMGKGLTAMIVVSDWIGARSPIDAVGLVSVQSVLLVTIALVI
                     ATMATTWLRVAAIPFALAGLLTVPALRSPDVLVSEDARLLAMPIGGGELAVNRARPNE
                     FTIDNWRRALDAESIVGPETTKAAGFDLADSLDLPTGTPFLCGDGLCVARHASGAIIA
                     HAQNADAARKACGFAALIVIDDATAADPCHDPLVAVITKRQLALSGSAEIFFQQQAAD
                     SPPEIQFAVEKHYRPWHIQRKFSRDARGLPPREKHSPTKTGHTGKTAKSADESPRLPY
                     PKDADQL"
     gene            complement(6401..7189)
                     /gene="lexA"
                     /locus_tag="VGM46_00970"
     CDS             complement(6401..7189)
                     /gene="lexA"
                     /locus_tag="VGM46_00970"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEY4191192.1"
                     /translation="MLTRKQHELLLFIHERLKESGIPPSFDEMKEALDLASKSGIHRL
                     ITALEERGFIRRLPNRARALEVLRLPDSLTPGFNAARKFSPSVIQGSLGQGNSSLGTA
                     NAAQGRGGQASGSGQPIKQPSRLQTAGNDDDAAANVSIPVMGRIAAGVPIDAIQHKTH
                     SISVPPDMIMGGDHYALEVKGDSMIEAGIFDGDTVIIRNANTANPGEIIVALVDEEEA
                     TLKRFRRKGASIALEAANPAYETRIFGPDRVKVQGKLVGLIRRY"
     gene            complement(7306..>7509)
                     /locus_tag="VGM46_00975"
     CDS             complement(7306..>7509)
                     /locus_tag="VGM46_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019858176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdenumtransferase MoeA"
                     /protein_id="HEY4191193.1"
                     /translation="AMAANDHRQDYIRATLREEEDGPVATPFGIQDSSMLRRLADAHC
                     LIVREPFAPAAEAGSACRVLMLR"
BASE COUNT         1298 a         2364 c         2372 g         1475 t
ORIGIN      
        1 cgggcggtgg cgatgcgccc atatgtcgac cgcatcctgt gcattttgcc gttcgagcaa
       61 ggcgagcttg cccggttggg cggcccgccg ggcatctatg tcggccaccg gctgagccat
      121 gatgccggcg tgctcgccgc cgccaaggcg caggcggctc cgcatgattt ttccactgag
      181 cgtgaaaaga cgctgcttat cctgcccgga tcgcgaagcg gcgaggtaaa caggctgatt
      241 ggcccgttcg gcgagacggt agcggcgctg cgcgcgcgcg gccaccattt gcggctatta
      301 ttgccaaccg tcccgcatgt cgctgaactg gtgcgctcct ccgtctccgg ctgggagcag
      361 cagcctgaga taattgtcga ccaggagcgg aaatggcagg cgttcgggca agccgatgct
      421 gcgttgatcg cctccggcac cgtctcgctt gaactggcgc tgtcgcgggt tccgatgatt
      481 tcctgctaca agctcgaccc gctggcgcgc cagcttgcgc gaagggtgat gataagctgg
      541 tcggcgtcgc tgccaaatct cattgccgac cagccgctgg tgccggagta ctacgacgat
      601 ttcgtccgtc cgcagatatt ggcgcgcgaa cttgaggcgc tgtttgccga cagcggcatg
      661 cgaagctggc agaaggaagg ctttgccgaa gtggcgcgcc gcatggcgac ggcgcagccc
      721 gctggcgaac tggctgccga ggccgtactt caacttgtcc ggaaataaaa aggcgccccg
      781 aaaggagcgc cttttcaaga tggtgccgcg caatcagcgc ttggcgatcg gcacgtagtc
      841 gcgcttcggc gagccggtat aaagctggcg tggacggccg atcttctgaa gcgggtcctc
      901 gatcatttcc ttccactgag agatccagcc gacggtgcgc gcgaccgcga acaggaccgt
      961 gaacatggtg gtggggaagc cgagcgcctt gagcgtgatg ccggaataga aatcgacatt
     1021 cggataaagc ttcttctcga tgaagtactc gtccgtcagc gcgatctttt ccagctccat
     1081 ggcgatgtcg agcagcggat cgtccttgat gccgagttcg ccaagcacct catgtgcggt
     1141 cttctgcatg atcttggcgc gcggatcgta gttcttgtag acgcggtgtc caaaacccat
     1201 caggcggaac gggtcgttct tgtccttggc gcgggtgatg aattccggga tatggtcgac
     1261 atggccgatc tggcccagca tgttcagcgc cgcctcgttg gcgccaccat gcgccggccc
     1321 ccacaggcag gcgatgccgg ccgcaatgca ggcgaacgga ttggcgccgg acgaaccggc
     1381 aagccggacc gtggaggtgg aggcgttctg ctcgtgatcg gcatgcagga tgaagatgcg
     1441 ctccatggcg cgcgccagca ccggattgac cttgtactcc tcgcaaggca ccgcaaagca
     1501 catatgcagg aagttcgccg cgaaaccgag atcgttcttc ggataaacga agggctggcc
     1561 gatgtgatac ttgaacgcca tcgcggctat cgtcggcatc ttggcgatga ggcgcatcga
     1621 ggcgatcatg cgctggtacg ggtcggaaat gtccgtggag tcgtggtaga aggccgacag
     1681 cgcaccaacc acgccgcaca tcacggccat cgggtgcgcg tcgcggcgga acccggtgaa
     1741 gaacttcgac atctgctcat gcaccatggt gtggcgcgtg atgcgatagt cgaaatcgtc
     1801 tttttgcgcc ttggtcggca gttcgccgta aagcagcaga tagcagacct caaggaagtc
     1861 gccatgttcg gcaagctggt cgatcggata gccgcgatgc atgaggatgc cggcgtcacc
     1921 atcgatgtag gtgatcgccg attcgcaggc tgcggtggaa gtaaagcccg gatcataagt
     1981 gaaggcgccg gtcgagctat acagcgaggc gatatcgatg acgtccggcc ccaccgagcc
     2041 acttcgcacc ttgaactcgt gtgacttgcc ttcaagttca agttttgcag ttttctcggt
     2101 catcgtaaat tccttcagct cttatatgca ggcttctcga ttatggcttc tgattgcaac
     2161 aggcgaacgt caaagccatg ttcgccgcgc ttggttccgg ttcgccccga atatgagggt
     2221 gcgcaatgtt gcaccgcaat agggctgttt caatccataa ttagggcagc atgcctgccc
     2281 taatcgattt gatctccaat acgtgaaagg ctttcgtcac gcccgagcac ggcaagcacg
     2341 tcaaacacgc ccggcgacgt gctttttccg gtgagcgcgg cgcgcaaggg ctgcgccacc
     2401 gcgccaagct tgtgtcccgc agtcgtggcg aattcgcgaa tcgccgcctc cgcgttcgcc
     2461 gcgttccatt cgccactaaa gtcgcccagc gcggcgtgcg ccccgcttaa gatagccttt
     2521 gcctcgccgc cgagcagcgc cgccgccttc tcatcgatgg gcaaggggcg ctccgcaaac
     2581 aggtagccgg cgccgtccgc cagttcgacc agcgtcttgg cgcgctcctt caggccgggc
     2641 agggctgcaa gcaattgcgc cttgcggccc gcatcgagac gcgccgcgat tgccggccca
     2701 ccttccagat agggcagcgt ttcgacgaag atggccatca gttcggcatc gtccatgcgg
     2761 cgcatatgaa cgccgttaag cgcctccagc ttggcgaagt cgaagcgggc cgcgcctttg
     2821 ttgacgtcgg taatgtcgaa ccatttgatc atatcggcga tcgacatgat ctcgtcgtcg
     2881 ccatggctcc agccgagccg cgccagatag ttgagcagcg cttccggcag atagcccatc
     2941 gcgcggtagg cttcgacgcc cagcgcgccg tggcgcttcg aaagcttggc gccgtccgcg
     3001 ccatggatca gcggtatatg cgccatatgc ggcaccgccc agcccatggc gttgtagatg
     3061 acggtctggc gcgccgcgtt ggtcaggtga tcatcgccgc gaatgatgtg ggtgacgtcc
     3121 atgtcgtggt cgtcgacgac gacggcatgc atgtaggtcg ggttgccatc cgaacgcagg
     3181 atgatgaaat cgtcgagatc cttgttggga aagcgcactt cgccctgcac gagatcgtgc
     3241 acgaccgtct cgccctcggt cggcgctttg atgcggatcg ccccggccac gcctgcgggc
     3301 gcttccgaag ggtcgcggtc gcgccagcgc ccgtcatagc gcggcggccg gccttcggcg
     3361 cgcgccctgt cgcgcatctc ggtcagttcg gcagggcttg catagcagta ataggccttg
     3421 cccaggcgca ccagctcttc agccacttcg cggtggcgcg gcgcgcgctc gaactgcgag
     3481 accgtttcgc cgttccattc gaggccaagc caggaaagcc cgtcgagaat ggcggcggtc
     3541 gcggcatcgg tggaacgctc gcggtcggtg tcttcgatgc gcagcagcat cgtgccgccc
     3601 agatgccgcg aatagagcca gttgaacagc gcggtgcgcg cgccgcctat atgcaggaaa
     3661 ccggtgggcg agggggcaaa acgggtgacg accttgtcgg acatggcgga ttctcgaagg
     3721 aaaggacttt tatctgcgcg ggctttcgcg cctcgcgcgt tcatgtagca taggaggtcg
     3781 agggcgcaag gggcagaccc gatggctgga gcgggccggg gcggaggcga gggcgctggc
     3841 gcgatcaaaa gcgccggtga gcgcgagctt ttcagcctcg atccggatat ccagggcagc
     3901 ccttatccgc cgccgcgctt gccgcaaact cagccgccgt taagcccgga gcgcgacagc
     3961 gctccagaag ctttgccggc caagggcggg cgcgacagtg cttcacacgg tttgccaacc
     4021 aagggcaggc gcgacagcgc tccagacggt tttccgacca ggggctggcg cgccggtaaa
     4081 aggctgcgcg ccatttccgt tggcgagttg ccgcgccttg ccctggttgc ggccgccacg
     4141 gaactcgacc ggggcgcggc cttcctgctg gtgccggtgt tcctcgccgt gggcgtggtg
     4201 ttctattttt cgctgagcgc ggagccgggt ctcttgcagc cggcggctgt ggttggcttg
     4261 ctggccggcc tggcgttcct ttcgaggcat cggccggtgg cgtatctggc ccttgtcgcc
     4321 gcaatgttgt gcgcgctcgg cttcgtcacc gccaagctcg agacgctgcg cgccgacaca
     4381 cagatgctcg gttcggaaat atcgacgcgc ctaaccggcc gggtcgtcgc cgtcgatgca
     4441 atggaaaccg gccgcatgcg ggtcaccctc gacgtgattt cgaccgcccg gccgacgctg
     4501 cgctacatac cggcgcgcgt gcgtgtgtcg gcgcgggaaa ttccgccggg aacccaggcg
     4561 ggttcggtgc tggtcggctt cgcccgcctg ttgccgccga ccggaccggt gcggccggac
     4621 agctacgatt tctcattcca aagctatttt gacggcattg gcggcagcgg ctttttcctg
     4681 accaatccga aactcgcggc ccttcagccc attccgctat cggcgcggat aaacgccgca
     4741 atagaaaatg cccgcgaagc gattgccggc catattcgcg acagcatcgg tggagccgag
     4801 ggcgagatcg ccgccgcgct gatcgttggc gtgcgcgccg gcattccgga tgccatcaac
     4861 gaggcgatgc gcaagaccgg catctatcac atcatctcga tttccggcct gcatatggcg
     4921 ttggtcgccg gcaccatcat ggcgatgctg cgcggcggct ttgcgctgtt tcccgaattc
     4981 tcctcgcgcc ggccggttaa gaaatatgcc gccgccgccg cgttattctc gatcgccgcc
     5041 tatctgctga tctccggcgt ggtggtggcg gcggagcgga gcttcattat gttggcggtg
     5101 atgctggttg ctgtcttgtt cgaccgtgcc gcgctgacga tgcgcaacct ggcgatttcg
     5161 gccattgccg tcatcgtcgt ctcgccgcat gaggtggtcg gccccagttt tcaaatgtcg
     5221 ttcgcagcca ccgccgccct tgtcggtgcc tatgccggct ggtccgatta tcgcgcaacg
     5281 aaagcaggcg gcccgccgcc gccgcgccgc tcatggcccg ccttcctcac ccggaaattc
     5341 ttcggcggca tgggcggcct ggcattgacc tcgatcatcg ccggctgcgc cacgaccttg
     5401 ttcgctatgt ggcatttcca gcgcgtggcg ccgctcagcc tgtttgccaa tctagcggtg
     5461 atgcccatcg tttcggtggt cgtcatgccg ttcgccgtgc tcagcgcgct ggcaatgccg
     5521 ttcggcgccg atggcccgct cctctatgtg atgggcaagg gcctgacggc gatgatcgtc
     5581 gtatcggact ggatcggggc gcgctcgccg atcgatgcgg tcggccttgt ctccgtccag
     5641 tcagtgttgc tcgtcaccat cgccctcgtc attgctacca tggcgacgac atggctgcgg
     5701 gtcgcggcca tcccttttgc gctcgccggt ctgctgacgg tcccagccct acggtccccc
     5761 gacgtgttgg tgtcggagga cgcccggctg ctggcgatgc ccatcggcgg cggcgaactg
     5821 gcggtcaacc gcgccaggcc aaacgaattc accatcgata attggcgccg cgccttggat
     5881 gcggagagca tcgttggccc ggaaacgacc aaagccgccg gcttcgacct ggccgacagc
     5941 ctagacttgc cgacgggtac gccgttttta tgtggcgatg gcctctgcgt tgcccgtcac
     6001 gcttcaggcg cgatcatcgc acatgcgcaa aacgccgatg cggcccggaa ggcctgcggc
     6061 tttgccgcgc tcatcgtcat cgacgatgcc accgccgccg acccgtgcca cgatccgctt
     6121 gttgcggtga tcaccaagcg ccagctggcc ctttccggca gcgctgaaat cttcttccag
     6181 caacaggcgg cggatagccc gcccgaaata caattcgccg tcgaaaagca ctaccgtccc
     6241 tggcacatcc agcgaaaatt ctcccgcgac gcacgcggcc tgccgccacg cgagaaacac
     6301 agtccgacga aaaccggcca caccggcaaa accgcaaagt cagcggatga aagcccgcgg
     6361 cttccttacc cgaaggacgc ggatcagctt taaacgacgc tcaatagcgc cggatcaacc
     6421 cgaccagctt gccctgcacc ttgacccgat ccggcccgaa aatgcgcgtt tcataagcgg
     6481 gattggcggc ttcgagtgca atggatgcgc ccttgcgccg aaaccgcttc agcgtcgcct
     6541 cctcttcatc gaccagcgcc acgatgatct cgcccggatt ggccgtgttg gcattgcgga
     6601 tgatgaccgt atcgccatcg aagatgcctg cttcgatcat cgaatcgccc ttgacctcca
     6661 gcgcgtaatg gtcgccgccc atgatcatgt cgggcggcac cgagatcgaa tgcgtcttgt
     6721 gctggatggc gtcgatggga acgccggccg cgatgcggcc cattaccggg atcgagacat
     6781 tggcggccgc atcatcgtca ttgccggctg tctggaggcg ggaaggctgc ttgatcggct
     6841 ggccggagcc gctggcctga ccgcctcgac cctgcgctgc attcgctgtt cccaggcttg
     6901 aatttccttg gccgaggctt ccctggatca cgctgggcga gaatttccgt gccgcgttga
     6961 agccgggcgt cagcgaatcc ggcaaccgaa gcacctccag cgcgcgcgcc ctgttgggca
     7021 gccgtcgaat gaagccccgt tcttccagcg ccgtaatcag ccggtggata cccgatttgg
     7081 aggccagatc aagcgcctcc ttcatctcgt cgaaggaagg cggaattccg ctttccttca
     7141 gccgttcatg gatgaaaaga agcagttcat gttgcttgcg cgtcagcatt gcggcccccg
     7201 gatcatcccg ttgaggaatc ggaaaaacaa aaccagaaca aacactatct gttccagttg
     7261 tgttccgcaa ccatttaaag tttaatgaat gtgttgacga tttgttcagc gtaacatcag
     7321 aacacggcag gccgagcctg cttctgcggc gggcgcgaag ggttcgcgca cgatgaggca
     7381 atgggcgtcg gccagcctgc gcagcatcga ggaatcctga attccgaacg gcgtcgcaac
     7441 aggcccgtcc tcctcttcgc gcagcgtcgc gcggatatag tcctgccggt gatcgttggc
     7501 ggccatggc
//
LOCUS       DASYCS010000023         4262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3917543,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000023 DASYCS010000000
VERSION     DASYCS010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3917543"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VGM46_00980"
     CDS             <1..217
                     /locus_tag="VGM46_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HEY4191194.1"
                     /translation="PSVLLKPEIYVTAALAGACLFTLGDLVGLTPLLSDAIGFAAAFF
                     VRGGGLKFGWAFAPYKSRPGRDPDDIA"
     gene            complement(329..1135)
                     /locus_tag="VGM46_00985"
     CDS             complement(329..1135)
                     /locus_tag="VGM46_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY4191195.1"
                     /translation="MDAIAAAPLGRIGNLEVRLARNEAEIAAAQEVRYRVFYEEMGAG
                     KGAPEGSDADRFDRVCDHLLVLDNSLAVPEHRRIVGTYRLLRQETAAASGGFYSESEF
                     ELRRLVKRHPAERFLELGRSCVLPDYRSKRTIEALWQGIWAYINHYEIGVMAGCASFP
                     GTVPAAHAEALTYLAHHCRTGQDWDVRAVAGRYHSMDLMPIEAVNAKAAIAAMPPLVK
                     GYLRVGARIGDGCVIDHDFSTVDVFIVMPVKEIGARYVNYYGGDGQRFAA"
     gene            complement(1336..3612)
                     /locus_tag="VGM46_00990"
     CDS             complement(1336..3612)
                     /locus_tag="VGM46_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY4191196.1"
                     /translation="MDRRMIVQSVAETRQASGTADAGFTAPARQQMEPPPLMPNLRRG
                     NRDPVSILTVCAIFFMAGLCYLMKTPPAVIVALLGIGACVLIVQTRLRRTVAQLNDAT
                     GRDRIEIEKLADRMWELQESEERFRGLIDALGDLVVHRDRAGKIVYANKVFTDLLRVD
                     SLDLPDKTLNDLDIDIGIVPDAAFSDHECLSSTDVVIRTAEGPRWFSWIELSVRDKDS
                     GAVSHRAIARDITARKRAESSLITARERAEYASQAKSRFLATVSHEIRTPMNGIMGMA
                     KLLADTSLSPEQRTYVGAVSTSASALLALIEDLLDYSKIEAGRFDPDPQPVSPREIGD
                     SIVELLTARAYGKGIGLACRATTDVPQTIIADPGRIRQVLLNLIGNAIKFTEAGGVLV
                     TISRIRTETTDHIRFTVADTGPGLQEKDRERIFEEFEQVDGTSTRAHGGAGLGLAISK
                     RLVLAMEGAIGVASTFGEGSEFTFDIPAQSPVETSANRETMLADRRMVILSENAIEAR
                     AIADTVHAHGGIAEIATSAAQAAALSVGCNVILIDAALEDGGARLLKHLRQTGFASCE
                     AIILIAPTDRGLLGDLRANGYSTFLARPVRGETLLRILVAGNAATLVPSQPDKHALVS
                     QPSHRTGLSVLIAEDNEINAMLARATLMKAGHHVEVVGNGKAAVDAVADAQQSNRYDV
                     ILMDLHMPVMDGLDAISVIRRHEEERGLPPVPIMVLSADGQENTRHAVLAHGASGFLT
                     KPLDPEALMMAVEQQAVA"
     gene            complement(3712..>4262)
                     /locus_tag="VGM46_00995"
     CDS             complement(3712..>4262)
                     /locus_tag="VGM46_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEY4191197.1"
                     /translation="IVPERGPVVVTGAAGGVGSVATSLLSSLGYHVIASTGRGAQSGY
                     LLDLGAAEIISRDELAQPGKSLAKERWAGGVDAVGSTTLAGVLSMTAYGGAVAACGMA
                     GGLDLPASVAPFILRGVSLLGIDSVRAPRELRLEAWRRIGADLDLGKLAALSTTIGFD
                     GIVQAAHDIVEGKVRGRVVVDM"
BASE COUNT          750 a         1314 c         1398 g          800 t
ORIGIN      
        1 gccatccgtc ctgctgaaac cggaaatcta tgtgacggcc gcccttgccg gcgcctgcct
       61 gttcacgctt ggcgacctgg tcggcctgac gccgctgctt tcggatgcaa tcggatttgc
      121 ggcggcattt ttcgtgcgcg gcggcgggct taaattcggc tgggcttttg cgccttacaa
      181 gagccgcccc ggccgcgatc cagacgatat tgcgtgacgg cttgctagag cggttccgat
      241 ttttgctgaa tcgacaaaac cgctctatct ctttgttttg gccgcatttc cgggcgcaaa
      301 accgtttcac acttttgctg gaaatgctct aggcggcgaa acgctgcccg tcgccgccat
      361 aataattgac gtagcgcgcg ccgatttcct tgaccggcat gacgatgaac acatcgaccg
      421 tggagaagtc gtggtcgatg acacagccgt cgccgatgcg cgcgccgacc cgcagatatc
      481 ccttgaccag cggcggcatc gccgcgatgg ccgccttggc gttgaccgcc tcgatcggca
      541 tcaggtccat cgaatggtag cgtccggcaa ccgcgcggac gtcccagtcc tgcccggtgc
      601 ggcaatggtg ggcaaggtag gtcagcgctt cggcatgggc cgccggcacc gttccgggaa
      661 acgacgcgca gccggccatg acgccgatct catagtggtt gatataggcc cagatgccct
      721 gccacagcgc ctcgatggtg cgcttggagc ggtagtccgg cagcacgcag gagcggccaa
      781 gttccaggaa gcgctcggct gggtggcgct tgacgaggcg gcgcagctcg aattcgctct
      841 cggaataaaa accgcccgag gcggcggccg tttcctgacg cagcagccgg taggttccca
      901 cgatgcgccg atgctcgggc accgcaaggc tgttgtcgag tacgagaaga tggtcgcaga
      961 cccgatcgaa ccggtcggca tcgctgcctt cgggcgcgcc tttgccggcg cccatttcct
     1021 cgtagaatac gcggtagcgc acttcctgcg cagcggcgat ctcagcttcg ttgcgggcaa
     1081 ggcggacttc gaggttgccg atgcggccga gcggcgctgc ggctatggca tccatcgcat
     1141 gccgttccac aagaggagcg ggggcgttcg cgtcgatctt gccttgcggc gtcgctgctt
     1201 gcacgagtgg tcctccttcg agccatccct cttggcgcgg gaatacgatt gtggtgcaac
     1261 aggattgtga cggcaacgtg aaaaaacgtg agacggcttt tatcctcaat ccatggttgt
     1321 gaaaccaggg attgatcagg cgacagcctg ctgttcgacc gccatcataa gcgcttccgg
     1381 gtcgagcggt ttagtcagaa aaccgctggc tccgtgcgcc agcaccgcat ggcgcgtatt
     1441 ttcctggcca tcggccgaca gcaccatgat cgggacgggc ggcaggcccc gctcttcctc
     1501 gtgacggcga atcacggaaa ttgcgtcgag gccatccatt accggcatgt ggaggtccat
     1561 caggatcaca tcgtagcggt tgctttgctg ggcatccgca accgcatcga cggcggcctt
     1621 gccgttgccg acgacttcga catgatgccc ggccttcatc aaggtggcgc gcgccagcat
     1681 ggcgttgatt tcattgtctt cggcgatcag caccgacagc cctgtccggt gcgagggctg
     1741 gctgacaagg gcatgtttgt ctggctggga cggaacaagg gtggcggcgt tgccggccac
     1801 gagtattcgc agcagcgttt caccccgcac cggccgcgcc aggaaggtcg aatagccgtt
     1861 ggcgcgcaag tctcccagca ggccacggtc cgtcggggcg atcaggatga tcgcctcgca
     1921 ggaggcaaag ccggtctggc gaagatgctt caacaggcgg gcgccgccgt cttccagcgc
     1981 agcgtcgatc aggatcacgt tgcagccgac ggaaagcgcc gccgcctggg ccgcgctagt
     2041 ggcaatctcg gcgatgcccc cgtgggcgtg aaccgtatcg gctatcgccc tcgcctcgat
     2101 ggcgttttcc gacaggatga ccatgcgcct gtcggcaagc attgtctcgc ggttcgccga
     2161 tgtttccacc ggcgattggg ccgggatgtc gaaggtgaat tcggacccct cgccaaatgt
     2221 gctggcaacg ccaatggcgc cttccatggc aaggacaagc cgtttggaaa tggccaggcc
     2281 gaggccggcg ccgccatggg cgcgggtcga ggtgccgtcg acctgctcga attcctcgaa
     2341 aatgcgttcg cggtcctttt cctgtagtcc agggccggta tcggcgacgg tgaaacggat
     2401 atgatcggtg gtttcggtgc ggatgcgcga gatcgtcacc aaaacgccgc cggcttcggt
     2461 gaatttgatc gcgttgccga tcaggttcag caggacctgc ctgattcttc ccggatcggc
     2521 gatgatcgtc tggggaacgt cggtcgtggc gcggcaggca aggccgattc ccttgccata
     2581 ggcgcgggcg gtcagcaatt cgacgatgct gtcgccgatt tcgcgtggcg acaccggctg
     2641 cgggtccgga tcgaagcggc ctgcctcgat cttggaataa tcgagcaaat cctcgatcag
     2701 ggcgagaagc gcgctggccg aggtcgagac cgcgccgaca taggttcgct gttccggtga
     2761 caggctggtg tcggccaaca gcttggccat gcccatgatg ccgttcatcg gggtgcgaat
     2821 ctcgtggctg acggtggcca ggaagcggga cttggcctgg ctggcatatt cggcccgttc
     2881 ccgcgcggtt atcagggaag attccgcgcg ttttcgcgcg gtgatatcgc gggcaatggc
     2941 gcgatgcgaa accgcgccgc tatccttgtc gcgaaccgaa agctcgatcc acgaaaacca
     3001 gcgcggcccc tccgccgtgc ggatcaccac atcggtggaa ctcaggcact catggtcgga
     3061 aaacgcggcg tcggggacga tgccgatatc gatgtcgagg tcgttcagcg tcttgtccgg
     3121 cagatctagg gaatctacac gcaggagatc ggtgaagacc ttgttggcat agacgatctt
     3181 gccggcgcga tcgcgatgga cgaccagatc gccgagcgcg tcgatcagcc cgcgaaagcg
     3241 ctcctcgctt tcctgcaatt cccacatgcg gtcggcgagt ttttcgattt cgatgcggtc
     3301 gcggccggtg gcgtcgttca attgcgccac cgtccgccgg aggcgggtct gcacgatgag
     3361 tacgcaagcg ccgatgccca gcagtgcgac gatgacggcc ggcggcgttt tcatgaggta
     3421 gcagaggccg gccatgaaga agatggcgca gacggtcagg atcgagacgg gatcgcggtt
     3481 gccgcgccgt aaattcggca tcagcggcgg cggctccatc tgctgccggg ccggtgcggt
     3541 gaagccggca tcggcggtgc cggatgcttg cctggtttcg gcgacggatt gcacgatcat
     3601 gcggcgatcc atgccataga gagattatgg aaatttgcgg cagatcgttc gtattttagc
     3661 gaacggccag atctctctca aacgaccaga tatctcatcg catgatttgg ctcacatatc
     3721 gacgacgacg cgcccgcgca ccttgccctc gacgatatcg tgcgctgcct ggacaatgcc
     3781 gtcgaagccg atcgtcgtcg acagggcggc gagcttgcca aggtcgaggt cggcgccgat
     3841 gcgccgccac gcctcaagtc ttaactccct gggggccctg accgaatcga tgccgagcag
     3901 cgagacgccg cgcaggatga atggcgcgac cgaggccggc aaatccaggc cgccggccat
     3961 gccgcaggcg gcaaccgcac cgccataggc cgtcatcgat agcacgccgg ccagcgtggt
     4021 cgagccgacc gcatcgacgc cgccggccca gcgttccttg gccagggatt tgcccggctg
     4081 cgccagctcg tcgcgcgaaa tgatttccgc cgcgccaagg tcaagcaggt agccgctttg
     4141 cgcgccgcgc ccggtcgagg cgatgacatg gtagccgagg ctggacagaa gtgaggtggc
     4201 gacggagccg acgccgccgg cagcgcccgt caccaccacc gggccacgct ccggcacgat
     4261 gc
//
LOCUS       DASYCS010000024         3269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3772902,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000024 DASYCS010000000
VERSION     DASYCS010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3772902"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1129
                     /locus_tag="VGM46_01000"
     CDS             <1..1129
                     /locus_tag="VGM46_01000"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006335353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEY4191198.1"
                     /translation="AKENAELANLSKTRFLAAASHDVLQPLNAAHLSVSALAEIQTSG
                     EGRKLVRQVERSLETMEDLLRTLLDISKLDAGVVRPDIDDVSLQAIFSSLRSDFQPLA
                     ELNGLTLKFRAANTAVRSDKTLLRRILQNIISNALRYTRSGGVLVGIRHRGDTVRIDI
                     ADTGCGIPDDQRDAVFEEFHRGSVSADASLAGGGLGLGLAIVRRIASALSHPVTFSSK
                     VGRGTIFHIDVPAGRLPAVDTEVAAELERPRGYGLFGTKVLLIENDPDVMQAMTSLFE
                     RWQCVVRQASTTDEALYKLGDTAWVPDIVIADQNLDGGDLGSHTIAEVRDYLGRMVPA
                     LIVTADPSELVVKAAKAAGIELMRKPLKPAQLRALLAHLLA"
     gene            complement(1133..1810)
                     /locus_tag="VGM46_01005"
     CDS             complement(1133..1810)
                     /locus_tag="VGM46_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY4191199.1"
                     /translation="MTASPDAVRFLIIDDHPLFREALHSAVQMAYPDVDTVEARSIAE
                     AMEILASGKAFDLALLDLSMPDVRGFDGLLQLRTRYPRLPVVIVSGHEEARIISEALS
                     YGAAGFIPKSARKSELAGAIRSVMDGAVFVPENYDGRSPDPDTTDRADLVSRLAKLTP
                     QQLRVLRMLRQGLLNKQIAYELQVGETTVKAHVSEILRKLNVYSRTQAVIEVSKLDSA
                     GLFRDQA"
     gene            2052..2855
                     /locus_tag="VGM46_01010"
     CDS             2052..2855
                     /locus_tag="VGM46_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEY4191200.1"
                     /translation="MNIQRRRVWWGDYRTTEYASIDPEATIAVLPVAAIEQHGPHLPV
                     STDTAIMEGMLETMIARLPADLDIRILPVQAVGKSNEHIASPGTLTLPPATLIDAWTE
                     LGLSVARAGVKKIVVVNSHGGNEEIMGIVTRELRVREKMLAVKTSWQRFGRPVGLYTD
                     LEDRQGIHGGDVETSLMLHFRPDLVDMGKARNFVSNVTRAEAEFTLLRHTGPHAFAWL
                     AGDLNPNGVVGDASAATAEKGRQTAEFQADGFITLLKDMRKAKLADWIS"
     gene            complement(2856..>3269)
                     /locus_tag="VGM46_01015"
     CDS             complement(2856..>3269)
                     /locus_tag="VGM46_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006335609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine phosphatase family protein"
                     /protein_id="HEY4191201.1"
                     /translation="TRPASIKPENHLHLAMNDIAEAQAGLTLPGEQHVRRLLDFARAW
                     DRSTPLVVNCYAGISRSTASAYIIAAALAPLRDEEELAQALRWLSPSATPNPRLIGVA
                     DNILGRDGRMVAAIKAIGRGADAFEGTPFIMEIDA"
BASE COUNT          645 a         1027 c          992 g          605 t
ORIGIN      
        1 cgccaaggaa aatgccgaac tcgccaatct ttccaagacg agattcctcg ccgccgccag
       61 ccacgacgtg ctgcaaccat tgaacgccgc gcatctttcg gtgtcggcgc tggctgaaat
      121 acaaaccagc ggcgaaggtc gcaaactcgt ccggcaggtc gaacgctcgc tggagacgat
      181 ggaagatttg ctgcgcacgc tgcttgatat ttccaagctc gatgccggcg tcgtccgccc
      241 cgatatcgac gatgtaagcc tgcaagcaat cttttcctcg ctgcggtccg atttccagcc
      301 tttggccgaa ctgaacggac taacgctgaa attccgcgcg gccaatacgg cggtccggtc
      361 ggataagacg ctgctgcgcc gcatcttgca gaacatcatc tcgaatgcgc tgcgctacac
      421 gcgctcgggt ggcgttctgg tcggcatccg ccatcgcggc gataccgtgc gcattgacat
      481 tgccgacaca ggctgcggca tcccggatga ccagcgcgac gccgtcttcg aagagttcca
      541 ccggggcagt gtttccgccg acgcttcgct tgccggcggc ggactgggcc ttggcctggc
      601 gattgtccgg cgcatcgcca gcgcgctttc ccacccggtc accttttcct caaaggttgg
      661 gcgcggcacg atctttcaca tcgatgtgcc cgccggcaga ctgccggccg tcgacaccga
      721 ggttgccgcg gaactggaac gcccgcgcgg ctacggcctg tttggcacca aggtgctgct
      781 gatcgaaaac gatcccgatg tcatgcaggc gatgacctcg cttttcgaac gttggcaatg
      841 cgtggtccgg caggccagca cgacggacga agcgctttac aagcttggcg acactgcctg
      901 ggtgcccgat atcgtcatcg ccgaccagaa ccttgatggg ggcgaccttg gcagccatac
      961 cattgccgag gtccgggatt atcttggccg catggtgccg gcgctgatcg tcaccgccga
     1021 tccgtccgaa ctcgtggtca aggccgctaa agcagccggc atcgagctga tgcgcaaacc
     1081 tttgaagccg gcgcagttgc gggctcttct tgcgcatctc ctggcctagg cttcaggcct
     1141 ggtcgcgaaa caggccggca ctgtccagct tggaaacctc gatcaccgct tgcgtgcggc
     1201 tatagacgtt gagcttgcgc aatatctccg agacatgcgc ctttacggtg gtttcaccta
     1261 cctgcaattc gtaggcgatc tgcttgttga gcagcccctg gcgcaacatg cgcagaaccc
     1321 gcaattgctg cggcgtaagc ttggcgagcc ttgagacaag atcggccctg tccgtcgtat
     1381 cgggatccgg cgaacggcca tcgtaatttt ccggcacgaa gacggctccg tccatcaccg
     1441 aacggatcgc gccggccagt tcgctcttgc gggcggattt gggaatgaag ccggccgctc
     1501 cataggaaag cgcttcggaa atgatcctcg cttcctcgtg gccggaaacg atcaccaccg
     1561 gcaggcgcgg atagcgggtg cgaagttgca acaggccatc gaagccgcgc acatcgggca
     1621 tcgaaaggtc gagcagcgcc agatcgaacg ccttgccgga ggccagtatc tccatcgcct
     1681 cggcgatgga acgcgcctcg accgtgtcga cgtccggata agccatctgc acggcgctat
     1741 gcagcgcctc gcgaaacagc ggatgatcgt cgataatgag gaatcggacg gcatctgggc
     1801 ttgcggtcat accggcactt cacttcagct gaaaaacaat ggtttcgaaa gataggacga
     1861 ttaccgacaa atagtacacc gccggcattt gtcgccgccg accggaattt ctgaagcgcg
     1921 ccctcccgct ggaccgcttg gcccgacaga gtgtaacaag actgacgatg cacagaaggc
     1981 gtgttgtcga tcgaggtcgg ccatgggcgt cataaccggg ttttgacggc aagctggcgg
     2041 aaaggcacga gatgaatatt caacggcgac gcgtttggtg gggcgactac cggaccaccg
     2101 aatatgcatc catcgacccc gaagcaacca tcgccgtgct gcctgtggcg gcaatcgagc
     2161 agcatggccc gcatctgccg gtttcgaccg acaccgccat catggaaggc atgctggaaa
     2221 cgatgatcgc gcgcttgccc gccgacctcg acatacgcat cctgccggtg caggcggtcg
     2281 gcaaatccaa cgaacatatc gcatcccccg gcacgctgac cctgccgccg gcgaccctga
     2341 tcgacgcttg gaccgagctt ggcctttcgg tcgcgcgcgc cggcgtgaag aagatcgtgg
     2401 tcgtcaactc gcatggcggc aacgaggaga tcatgggcat cgttacccgt gaactgcgcg
     2461 tgcgcgaaaa gatgttggcg gtaaagacaa gctggcagcg tttcggccgc ccagtgggcc
     2521 tttataccga cctggaagac cggcagggca ttcatggcgg cgatgtcgag acatcgctga
     2581 tgctgcattt ccgtcccgat ctggtggaca tgggcaaggc gcggaatttc gtgtcgaacg
     2641 taacccgcgc cgaagccgaa tttacgctgc tgcgccacac aggcccccac gccttcgcct
     2701 ggctggccgg cgatctcaac cccaacggcg tggttggcga cgcctccgcc gcgaccgccg
     2761 aaaagggccg gcagacggcc gaatttcagg cagacggctt catcaccttg ctgaaagaca
     2821 tgcgcaaggc aaagcttgcc gactggattt cctagtcagg catcgatttc cataatgaac
     2881 ggcgtgcctt caaaagcgtc ggcgccacgg ccgatggcct ttatcgccgc aaccatgcgt
     2941 ccatcgcgcc caaggatgtt gtcggcaacc ccaatcagcc tgggattggg ggtcgccgaa
     3001 ggtgacagcc agcgcaacgc ctgcgccagt tcttcctcat cgcgcaaagg ggcaagcgcg
     3061 gccgcgatga tataggccga ggccgtcgag cggctgatgc cggcatagca attcaccacc
     3121 agcggcgtcg aacgatccca ggcgcgggcg aaatcgagca ggcggcgcac atgctgctcg
     3181 cccggcaatg tcagcccggc ctgcgcctcc gctatgtcgt tcatggcgag atggagatgg
     3241 ttttcgggct tgatcgacgc cgggcgtgt
//
LOCUS       DASYCS010000025         8657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4932202,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000025 DASYCS010000000
VERSION     DASYCS010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 8657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8657
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4932202"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..2063
                     /gene="thrS"
                     /locus_tag="VGM46_01020"
     CDS             81..2063
                     /gene="thrS"
                     /locus_tag="VGM46_01020"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEY4191202.1"
                     /translation="MSEVSLAFPDGSVRVYDAATTGAQLAESISKSLAKKAVAYAMDG
                     TLRDLSDPLGASGKVEIVTRDDPRALELIRHDAAHVLAEAVQELWPGTQVTIGPVIEN
                     GFYYDFARNEPFSPDDFPIIEKKMREIIGRNKPFTKEVWSREKARKVFSDKGEAYKLE
                     LIDAIPEDQDLKIYAQGDWFDLCRGPHMASTGQVGNAFKLMKVAGAYWRGDSNNPMLT
                     RLYGTAWADQAQLDEYLHMLEEAEKRDHRKLGREMDLFHFQEEGPGVVFWHAKGWKMF
                     QNLISYMRRRLGDDYQEVNAPQVLDKSLWETSGHWGWYQENMFAVKSAHAFTHPDDPE
                     ADQRIFALKPMNCPGHVQIFKHGLKSYRDLPIRLAEFGNVHRYEASGALHGLMRVRAF
                     TQDDAHIFCTEEQLEAEILKINDLMMSVYRHFGFDEVVVKLATRPEKRVGTDAAWDHA
                     EDILRRALERIASHDNRIKTGINEGEGTFYGPKFEYTLKDAIGREWQCGTTQVDFNLP
                     ERFGAFYIGSDSEKKQPVMVHRAICGSLERFLGILIESHAGQMPLWFAPLQVVVATIT
                     SDADDYAVKVVERLKAAGLSAQADLRNEKINYKVREHSLAKVPVMFVCGKREAEEETV
                     NIRRLGSRDQQSLGLGEAVAALVQEAVAPDLKAKIA"
     gene            2152..3093
                     /locus_tag="VGM46_01025"
     CDS             2152..3093
                     /locus_tag="VGM46_01025"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY4191203.1"
                     /translation="MSYHRPHREMFPELSYANSGQPALTRWFIRTVERLSGRDRFAEL
                     YDFWRRTVAPTGEHVFGRMLNLIDVRVNGAEQWPPAVLPNAPLVIVANHPFGIGDGIA
                     VLSLAEKLGRPFRVMIHKDLLRVREMEPYSLPVDFSDTKEALKNNMAVRHEAVRLLKE
                     GVVIVVFPAGGVSTAPRGIGRARDLPWKMFPARLVQDAKACVIPMHFSGQNGRLFHLV
                     SAPMNLAERNGRLARAVGKASITLRISLLIGEFARLAGKSIDVSIGAPLGWEELEPLR
                     DRKELLGRLHCGVFDLAPAMPIRQRPRFRGRLRRAAA"
     gene            complement(3194..4159)
                     /locus_tag="VGM46_01030"
     CDS             complement(3194..4159)
                     /locus_tag="VGM46_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015317584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191204.1"
                     /translation="MIEGISRLLPIILAAAFLVAPVSHAGAQDKDALGKLKLPGISNY
                     ANPKHEGWSAANGGGEITLSAQLTDKGADITRGMVWRVFQPKADETGKLPLVASAHGG
                     TAKFELEPGSYLVHAAYGRAGATKRITVGKDARHESLVLDAGGLELNAVLSGGARIPH
                     EKLRFSIYEGQPTAGNDGTLILPNVQPGTVVRLNAGTYHVVSNYGSVNAVIRSDIRVE
                     AGKLTEATVEHRAAEITMKLVREAGGEAIADTSWSVLNESGDTIKEAVGAFAPMVLAE
                     GSYTVIAKNRDRIYQRDFSVEAGKNDDVEVIASDDNAIDPDEGAD"
     gene            4279..4863
                     /locus_tag="VGM46_01035"
     CDS             4279..4863
                     /locus_tag="VGM46_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HEY4191205.1"
                     /translation="MTSPIVDFLLARSSAPIPDLHAPGPSDEQIATMIAIASRVPDHG
                     RLAPWRFILYRGDARSEIGKKLAALAEQREGPLTEGRRNQELTRFSRAPLVVGVISSP
                     KENVKIPQWEMFLSGGMAAMNLMLAANGLGFGTQLITNWYSGVPEGRAILGLSPQEQV
                     VGFVHVGSHDGPVPERPRPDPATLYADYSGPWTE"
     gene            complement(4839..5735)
                     /locus_tag="VGM46_01040"
     CDS             complement(4839..5735)
                     /locus_tag="VGM46_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019858443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEY4191206.1"
                     /translation="MRSLLFVPGDSEKKLEKGFSSEADVVIVDLEDSVSPQNKAAARQ
                     TAAQFIAAREKTPGPTIYIRINDLSTGLTDDDLKALVPARPDGIMLPKSNSGSDVERL
                     SAKLRVHEAENGLPDGAIKILPIITETGAGVLAAASYADAGARLSGLTWGAEDLSAAI
                     GARATRDANGAYTDVFRLARSLTILAASSAQVAAIDTVFPNFRDMAAFEAECFEAERD
                     GFTGKMAIHPAQVPAINAAFTPSAQAIEHSMAVVAAFAAAGNPGVVGLDGKMVDRPHL
                     RLAERLLARAKAAGVYSVHGPE"
     gene            complement(5739..6191)
                     /locus_tag="VGM46_01045"
     CDS             complement(5739..6191)
                     /locus_tag="VGM46_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEY4191207.1"
                     /translation="MAGLYLEELAAGQVFRHTLRKTVTESDNMLFSAMTLNPQPLHID
                     FDFASKTEWGKPLVNSLFTLGLMIGISVNDITVGTTLANLGMKETVFPHPVFHGDTIH
                     VETTVVSVRESKSKPDRGIVEFEHRAYNQADILVASCTRQAMMLKRPA"
     gene            6370..7494
                     /locus_tag="VGM46_01050"
     CDS             6370..7494
                     /locus_tag="VGM46_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019858439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HEY4191208.1"
                     /translation="MVVVSHFLKWIYTARVSERAAAAGVLAHAYIDAALPFEDRCAAE
                     AALTLLLDDPSAKVRLAMAEALSMSHHAPIQVIAALASDQPEVASLVLARSPLLTDAD
                     LIDRVATSQKATQTLIAGRPVVSMAVAAAIAEIGEQEACAALLANGGAEIASFSFRRM
                     AERHGHLPYLREALIAHARLPADCRHMLLIKLGETLKTSPLVLALMGAARAERIMRDA
                     CVKASLTLIEGIRAEEHSALIEHLRLRGDLTASFIIRTIAHGKVDFFGSALVALTRQS
                     EQRVRALLACGHDVALHALFRSAGLAAATHAIILRALKVWREVANGKRLAGVQEVTWL
                     MLKELGGQAAEGDLAGLVKSMHLDALRENARGHALAIAAA"
     gene            complement(7629..>8657)
                     /locus_tag="VGM46_01055"
     CDS             complement(7629..>8657)
                     /locus_tag="VGM46_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEY4191209.1"
                     /translation="ALQAPAARRVRERRQPALIIHRDHHAAWRNAARATVATMLMAAF
                     WLTTQWAEAAGIVIIVAVVASLFAPRPSPVQVAWGFFEGTLLALPFAFLVGQILLPAL
                     PGFGWFILFVVPILVPAALAMANPRLVGVATAFAINFLAFLSPQKVMVYNPVEFLGSA
                     ASILIGILLAIGVFLVVLPANPWKTANHIAQAICEDLVRLCLHERIPRRSAFESLAYD
                     RVNQMMALMQPPGRKGDVFLGGGIMAVTVGLEILRLRNTVQAGAISTETSQAIKKFLK
                     RLAYELLTPKSPPEWQASIDAIRQKAETIAAEDHDMGILRVAASMRVIAAAIENYPEF
                     FNARAAAA"
BASE COUNT         1584 a         2618 c         2785 g         1670 t
ORIGIN      
        1 actaaaacac cgctcgtcgc cggacgcatc cggcatatcg ccacccgcgc tcgtttgcgg
       61 gactggatag gagaaaaccg atgtccgaag tttcccttgc atttcccgat ggttcggtcc
      121 gcgtctatga tgcggcgacg acgggtgcgc aactggctga atccatttcg aaatccttgg
      181 ccaagaaagc cgtcgcctat gcgatggacg gcacgctgcg cgatctttcc gatccgctcg
      241 gcgcgtccgg caaggtcgag atcgtgacgc gcgacgaccc gcgcgcgctg gagcttatcc
      301 gccacgatgc ggcgcatgtg ctggccgagg cggtgcagga actttggccg ggaacgcagg
      361 tcaccatcgg accggtgatc gagaatgggt tttattatga tttcgcccgc aacgagcctt
      421 tttcgcccga tgattttcca atcatcgaaa agaagatgcg tgaaatcatc ggccgcaaca
      481 agccgttcac caaggaagtg tggtcgcgcg agaaagccag gaaggtattt tccgacaagg
      541 gcgaggccta caagcttgaa ttgatcgacg ccattcccga ggaccaggat ctcaagatct
      601 atgcccaggg ggactggttc gacctctgcc gcgggccgca catggcctcg acggggcagg
      661 tcggaaacgc cttcaagctg atgaaggtgg ccggcgctta ctggcgcggc gattcgaaca
      721 atccgatgct gacgcgcctc tacggcacgg cttgggccga tcaggcgcag ctcgacgaat
      781 atctccacat gctggaggaa gccgagaagc gcgaccatcg caagctaggg cgcgagatgg
      841 acctgttcca cttccaggag gaggggccgg gcgtcgtctt ctggcatgcc aagggctgga
      901 agatgttcca gaaccttatt tcctatatgc gccgccggtt gggcgacgac tatcaggagg
      961 taaacgcgcc gcaggtgctc gacaagtcgt tgtgggaaac gtcgggccat tggggttggt
     1021 atcaggagaa catgttcgcg gtgaagtcgg cgcacgcctt cacgcaccca gacgaccccg
     1081 aggccgacca gcgcatcttc gcgctgaaac cgatgaattg ccccggccat gtgcagatat
     1141 tcaagcacgg cctgaaatcc taccgcgacc tgccgatccg gcttgccgaa ttcggcaatg
     1201 tgcatcgcta cgaggcttcc ggggcgctgc atgggttgat gcgcgtgcgc gccttcacgc
     1261 aggacgacgc gcatattttc tgcaccgaag aacagcttga ggcggaaatc ctcaagatca
     1321 acgacctgat gatgtcggtc taccggcatt tcggcttcga cgaagtggtg gtgaaactgg
     1381 cgacgcggcc ggaaaagcgg gtcggcaccg atgccgcatg ggatcacgcc gaggacattt
     1441 tgcgccgagc gctcgaacgc atagccagcc acgataaccg catcaagacc ggcatcaatg
     1501 aaggcgaggg cacgttctat gggccgaagt tcgaatacac gctgaaggac gccatcggcc
     1561 gtgagtggca gtgcggcacc acgcaggtgg acttcaacct gccggagcgg ttcggggctt
     1621 tctacatcgg ttcggattcg gagaagaagc agccggtgat ggtgcaccgc gccatctgcg
     1681 ggtcgctgga gcggttcctg ggcatcctca tcgagagcca tgccggccag atgccgctgt
     1741 ggttcgcgcc gttgcaggtg gtggtcgcca cgatcacctc ggacgccgac gattatgcgg
     1801 taaaggtagt ggagcggctg aaggcggctg gtctttcggc gcaggcagac ctgcgcaacg
     1861 aaaagatcaa ctacaaggtc cgcgagcatt cgttggccaa ggtgccggtc atgttcgtct
     1921 gcggcaagcg cgaggccgag gaagagacgg tcaatatccg ccggctcggc tcacgcgacc
     1981 agcagtcgct tggtctcggc gaggcggtcg cggctcttgt ccaggaagcc gtcgcccccg
     2041 atctcaaggc caagatcgcc tgatccgaac tgacggcaca aatacggcgg cggtggcgcg
     2101 atccaccgcc gttttcatat gcccgtcatg ctgggccaat aagctttccc gatgtcatat
     2161 caccgaccgc accgggaaat gtttcccgaa ctgtcctacg ccaattccgg gcagccggcg
     2221 ttgacgcgct ggtttatccg aaccgtcgag cgcctgtcgg ggcgtgatcg cttcgccgag
     2281 ctttatgatt tctggcggcg taccgtcgcg ccgaccggcg agcatgtttt cgggcgaatg
     2341 ctgaacctca ttgatgtgag ggtcaacggc gccgaacagt ggccgccggc ggttttgccc
     2401 aacgcgccgc tggtgatcgt ggccaaccat ccgttcggca tcggcgatgg catcgccgtt
     2461 ttgtcgctgg ccgaaaagct cgggcggccg ttccgggtca tgatccacaa ggacttgctg
     2521 cgggtccgcg aaatggaacc ctattcgctg ccggtggatt tttccgacac caaggaagcg
     2581 ctcaagaaca acatggcggt gcgccatgaa gcggtgcgcc tgcttaaaga gggcgtggtg
     2641 atcgtggttt ttccggcggg tggcgtctcc acggctccca gggggattgg ccgcgccagg
     2701 gatttgccgt ggaagatgtt tccggcgcgg ctggttcagg atgcgaaagc ctgcgtcatc
     2761 ccgatgcatt tttccgggca gaacggccgg ctattccatc tggtcagcgc tccgatgaac
     2821 cttgccgaac gcaacggccg gctggccagg gctgtcggca aggcctcgat caccttgcgg
     2881 atttcgctgc tgatcggcga attcgcgcgg ctggcgggca agtcgatcga cgtcagcatc
     2941 ggtgcgcccc tcggttggga agaactcgaa cctctgcgcg accgcaagga actgcttggg
     3001 cggctgcatt gcggggtttt cgatctggct ccagccatgc ccatcaggca aaggccccgt
     3061 tttagaggcc ggcttcgccg tgcggcagcc taaagtggtt ctggtttttg ccgaatcgac
     3121 aaaaccgctc catctctttg ttttggctgc atttccggac gcaaaaccgt ttcacgcttt
     3181 tgctggaaat gctctaatcg gcgccttcgt cgggatcgat ggcgttgtcg tcggaagcaa
     3241 tcacctcgac atcgtcgttc ttgccggcct cgacggaaaa atcgcgctgg tagatacggt
     3301 cgcgattctt ggcgatgacg gtgtagctgc cctcggccag caccatcggg gcgaaagcac
     3361 cgaccgcttc cttgatggtg tcgccggatt cgttcagcac cgaccacgaa gtgtcggcga
     3421 tggcctcgcc gccggcctcg cgcaccagct tcatggtgat ttcggcggcg cggtgctcga
     3481 cggtcgcttc ggtaagtttg ccggcctcga cgcgaatgtc ggagcggatg actgcgttca
     3541 ccgatccgta attggagacg acatggtagg tgccggcgtt cagccgcacg accgttccgg
     3601 gctggacgtt ggggagtatc aacgttccat cgttgccggc ggtcggctgt ccttcataga
     3661 tggagaagcg cagcttttca tgcgggatgc gcgcgccgcc cgacaatacg gcgttgagtt
     3721 caaggccgcc ggcatcgaga acgagcgatt cgtggcgcgc atccttgccg accgtgatgc
     3781 gcttggtggc gcccgccctg ccataggccg cgtggaccag atagctgccc ggctcaagtt
     3841 cgaatttcgc cgtgccgcca tgcgcggagg cgaccagcgg cagtttgccc gtctcgtcgg
     3901 ccttcggctg gaacacccgc cacaccatgc cgcgcgtaat gtcggcgccc ttgtcggtca
     3961 gttgcgcgct aagcgtgatc tcgccgccgc cattggccgc cgaccatcct tcgtgcttgg
     4021 ggttggcgta gttggagatg ccgggcagtt tcagcttgcc cagcgcgtcc ttgtcctgcg
     4081 cgccggcatg cgaaaccgga gcgaccagga aagcggctgc caggataatc ggcagaagac
     4141 gagaaatccc ctcaatcatg cttgcgttga agcccaaggc ggtggcaatt tcaaggccta
     4201 ttctccggcg catccgcgcc atcgccaaag ccagcctgtc gccagcgctt cggttcaacc
     4261 cacaggaaag ctgcgcccat gacctcaccg atcgtcgatt tcctgttggc cagaagttcc
     4321 gcgccgattc ccgatctgca cgcaccggga ccgagcgacg aacagatcgc cacgatgata
     4381 gccatcgcct cgcgcgttcc cgatcatggc cggctggcgc cgtggcgttt catcctctat
     4441 cgcggcgatg cccgcagcga aatcggaaag aagctggccg cccttgccga gcagcgcgaa
     4501 ggaccgttga cggaaggccg ccgcaatcag gaactgacgc gtttttcgcg cgcgccgctg
     4561 gttgtcggcg tcatttccag cccgaaggaa aacgtgaaga tcccacaatg ggaaatgttt
     4621 ctgtccggcg gcatggcggc catgaacctg atgctcgccg ccaacgggct gggcttcggc
     4681 acgcagctca tcaccaattg gtattccggc gtgccggagg gcagggcgat actcgggctt
     4741 tcgccgcagg aacaggtggt cggcttcgtc catgtcggtt cgcatgacgg ccccgtgccg
     4801 gaacggccgc ggcccgaccc ggcaacgctt tatgccgatt attcggggcc gtggacggaa
     4861 tagacgccgg cggcttttgc ccgcgccagc agacgctcgg ccagccgcag atgcggccgg
     4921 tcgaccatct tgccgtcaag gccgacgacg cccggattgc cggctgcggc gaaagccgcg
     4981 acgaccgcca tggaatgctc tatggcctgc gccgatgggg tgaaggcggc gttgatggcc
     5041 ggcacctgcg cggggtggat ggccatcttg ccggtaaagc cgtcgcgctc ggcctcgaag
     5101 cattcggctt cgaaggcggc catgtcgcga aagttcggaa atacggtgtc gattgccgcc
     5161 acttgcgccg acgatgccgc cagaatggtc agcgagcggg caaggcggaa aacgtcggta
     5221 taagctccgt tcgcgtcgcg ggtggcgcgg gcgccgatgg ccgccgaaag gtcttccgcg
     5281 ccccaggtca ggcccgaaag ccgcgcgccc gcatcggcat agctggcggc ggcgagcacg
     5341 ccggctccgg tttcggtgat aatcggcagg atttttatcg cgccgtccgg caatccgttt
     5401 tcggcctcgt gaaccctcaa tttcgccgaa agccgctcga catcgctgcc gctgttcgat
     5461 ttcggcagca tgatgccatc gggccgtgcc ggaaccagcg ccttcagatc gtcgtcggta
     5521 agcccggtgg agagatcgtt gatgcgaata tagatggtgg ggccaggggt tttttcgcgc
     5581 gcggcgatga attgcgcggc ggtttggcgc gcggcggcct tgttttgcgg cgaaacggag
     5641 tcttcgaggt cgacgatcac cacgtccgcc tcgctcgaaa atcccttttc gagtttcttt
     5701 tccgaatcgc ccggtacgaa cagcaaggag cgcatggttc aggccggcct cttcagcatc
     5761 atcgcctggc gggtgcaact ggccaccaga atgtcggcct ggttataggc gcggtgttcg
     5821 aattcgacga tgccgcgatc cggtttcgat ttcgattccc gcaccgaaac gacggtcgtt
     5881 tcgacatgga tggtgtcgcc gtggaaaacg ggatgcggaa agaccgtttc cttcatgccg
     5941 agattggcga gcgtcgtgcc gaccgtaatg tcgttgacgg aaatgccgat catcaagccc
     6001 agcgtaaaca gcgaattgac cagcggcttg ccccattcgg ttttggaggc gaagtcgaaa
     6061 tcgatgtgca gcggctgcgg gttcagcgtc atcgccgaaa acagcatgtt gtcgctttcg
     6121 gtcaccgtct tgcgcaacgt gtgcctgaag acctgcccgg ccgcgagttc ttccaggtaa
     6181 agtccggcca tggtcgatct cctcggtcgc tttgatagag gcccggtgca gcgcccgcaa
     6241 gcacgcagat tgccttgccg gagccggccc gtagtggacc ttaacgaata tggtgaaccg
     6301 tttgtaaatg ttttgcgaat acgctccgcc agggtcggaa aagaatttgg ccggggcaag
     6361 cgggcaagca tggtggtcgt ttcgcatttc ctgaaatgga tatatacggc gcgcgtttcg
     6421 gagcgggcgg cggcggctgg tgttctcgct catgcctata tcgatgcggc attgccgttc
     6481 gaggatcgct gcgccgccga ggccgcgctg acgcttctgc tcgacgaccc atcggcaaag
     6541 gttcgcctgg cgatggcgga agccttgtcc atgagccatc acgcgccgat ccaggttatc
     6601 gcagcccttg cctccgatca gccggaggtc gccagcttgg tattggcccg ctcgccgctg
     6661 cttaccgatg ccgatctcat cgaccgcgtt gcaaccagcc aaaaggcgac gcagacgctg
     6721 atcgccggcc ggccggtggt ttccatggcg gttgccgcag ccattgccga aatcggggag
     6781 caggaagcct gcgctgccct gcttgccaat ggcggcgcgg aaatcgcgtc gttcagtttt
     6841 cgccgcatgg ccgagcgcca cggccacctg ccttatttgc gggaagcctt gatcgcccat
     6901 gcccgcctgc cggcggattg ccggcacatg ctgttgatca agcttggaga gacgctcaaa
     6961 acgtcgccgc tggtcctggc gctgatgggc gcggcgcggg ccgaacgcat catgcgcgat
     7021 gcctgcgtga aggcctcgct caccttgatc gagggcattc gcgccgagga acattccgcc
     7081 ttgatcgagc atctgcgcct gcgcggcgac ctcaccgcca gcttcatcat ccgcaccatc
     7141 gcacatggca aggtcgactt tttcggctcc gccctggtgg cgttgacccg ccagtccgaa
     7201 cagcgggtga gggcgctgct ggcctgcgga cacgacgtgg cgctgcacgc gcttttccgc
     7261 agcgcgggac ttgcggcggc gacacatgcg atcattttgc gcgcgctgaa agtatggcgc
     7321 gaggtggcca acgggaaaag gcttgccggc gtacaggaag tcacatggct gatgctgaag
     7381 gaactcggcg gacaggccgc cgagggtgat cttgcggggc tggtcaaatc catgcacctc
     7441 gacgcgctgc gcgaaaatgc acgcggccat gcgctggcga tcgccgccgc ctagagcggt
     7501 tctggtttta tcgaatcggt aaaaccgctc tatctctctg ttttggccgc atttccggac
     7561 gcaaaccgtt tcacactttt gctggaaatg ctctagagtc gcatcttcat ccgttcgggt
     7621 cggccccgtc aggctgccgc tgccctggcg ttgaagaatt caggataatt ctcgattgcg
     7681 gcggcaatga cacgcatcga cgcggcgacg cgcaggatgc ccatatcgtg gtcttccgcc
     7741 gcaatggttt cggccttctg ccggatcgca tcgatcgaag cctgccactc cggcggggat
     7801 ttcggggtca gcaattcata ggcgagcctc ttcaggaatt ttttaatcgc ctgtgaggtt
     7861 tcggtggaaa tggccccggc ctgcaccgta ttgcgcagcc gcaggatttc gagaccgacg
     7921 gtaaccgcca tgatgccgcc gccgaggaaa acgtcgcctt tccggccggg cggctgcatc
     7981 agcgccatca tctggttgac gcggtcatag gcgaggcttt cgaaggcgga gcgtcttgga
     8041 attcgctcat gcaggcaaag ccgcaccaaa tcctcgcata tcgcctgtgc aatatggttt
     8101 gcggtcttcc agggattggc cggcagaacg accagaaaga cgccgatggc gagcaggatg
     8161 ccgatcagga tcgaggctgc gctgcccaga aactcgaccg ggttgtagac catcaccttt
     8221 tgcgggctga ggaaggccag gaaattgatg gcaaacgcgg tcgccacgcc gaccaggcgc
     8281 ggattggcca tcgccagcgc cgccggcacc agaattggaa cgacgaacag gatgaaccag
     8341 ccgaagcccg gcagggcagg cagcagaatc tgcccaacca gaaaggcgaa gggcagggca
     8401 agcagcgttc cttcgaagaa gccccaggca acctgcaccg gcgatggccg gggcgcgaac
     8461 aggctggcga cgaccgcgac gatgatgacg atgcccgccg cctcggccca ctgggtggtc
     8521 agccagaacg ccgccatcag catcgtggcc acggtggcgc gcgcggcgtt gcgccatgcg
     8581 gcatgatggt cgcggtggat gatcagcgcc ggctggcggc gttcgcggac ccggcgggcg
     8641 gcgggcgcct gtagcgc
//
LOCUS       DASYCS010000026         4363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1743363,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000026 DASYCS010000000
VERSION     DASYCS010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4363
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1743363"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="VGM46_01060"
     CDS             <1..622
                     /locus_tag="VGM46_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HEY4191210.1"
                     /translation="NRIGSKFLHAGPGYGGSCFPKDTLAFARTGQDFGCPLRLVETTV
                     VVNDQRKRAMARRIIAACGGNVRGRRIAVLGLTFKPNTDDMRDAPSLAIVQALVDAGA
                     VVRAYDPQGMEAAKPLLPPITYAQDAYAAADAAECIVIVTEWNEFRSLDFERLKAVMK
                     TPVLVDLRNIYNPAELARYGFAYESIGRGKDRNGDQAPARVIRLSA"
     gene            complement(647..2638)
                     /locus_tag="VGM46_01065"
     CDS             complement(647..2638)
                     /locus_tag="VGM46_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEY4191211.1"
                     /translation="MKAGDLPAAYFPATGAAAARHVFYVSPQGNDRWSGAAPLADAQR
                     ASGPFATLETARDSARKAGGPATIVMGGGDYYLASPVVFGPQDAGLAVLAKCGETPVL
                     HGGPPVTKWSSQGGGRWVAPLRLPSGQSVGDLFVDGIVQDEARYPNAPDDNDPRKGWL
                     FADKPDVDAWLGNIRFRFRPGDLPALKDAAGLVAHIVGGFLPGSQWGSDTLPVLSIDA
                     GTRTIHTGGTAYFFSAEGSRYFLSGSRALLDAPREWWYDATAGQLSYMPATPSFTSAK
                     IVAGVLPTFLRLEDADGMTISGLTFRDGAPQGSGKYGTDMRGFGAIRLEHSDGVRLVG
                     NVIDNVGVGIHVSESENVLIAHNEIGHVAGDGIYIGTVYGKFGKSSGAKVLANHIHDI
                     GRVYFESSGIWFHAADNVRIAGNLVEDTAQLGIAGGSIWESNDAIHNAVIEHNEVRNA
                     NRQTADGGAIKLMGTQADLQNSIIRANVVTGTRQLMNRADGTFWPTDYENTDEWPSPI
                     SWAIYTDLKASGVRIEGNVLCGNVAAIGINGGWNNVVTGNFIAQGSGAAFRLDDGTGR
                     DWKPAWAKPNRIEDNTVSIGGGGLAAAVYAPGHGSAYGEFRQNRYSGDLGDRSFKIEP
                     EIMPSGKYGSLADFQKEGGESGSILLPAQAADDAAKYCR"
     gene            complement(2770..4086)
                     /locus_tag="VGM46_01070"
     CDS             complement(2770..4086)
                     /locus_tag="VGM46_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003595485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEY4191212.1"
                     /translation="MKALSVRETGQLTVSWRTIEWWGAGICLFLQTGALFPLLLADPN
                     GDLGDAARAKLRFVSLPIYAFSILILSRHSRQFLVALKRNLLIHFLFAMPFVSVLWSV
                     SPGTTLRRANGLLFAVLLAYVLALRFTPRQLLLLLMMTLGACIVPSLLLLGISPGLAR
                     MPLDGALRGIFIHKNTLGWYASVLTLISFIVLTNGSYGHRRSAIALLIASIACLIGST
                     SMTSIIATASACCLTWFYTVLPKTRGIGRAAFVMLFVQMAAVLLILLHEFLVPVLEAL
                     GKDATLTGRVPLWELVDAEIGRHLLLGFGYQAFWTEANPEAWSIWSQIQWMAPHAHNG
                     FRDTLLSFGIGGMCLFMLVVIRAAYQGAVLQCRAPEDGWLWLNVFMVMVMVMNLTESI
                     FLVQNDTIFVLFATAIILFSLYSPVYAKKPAPHAGYASLPAGLQIR"
BASE COUNT          815 a         1384 c         1424 g          740 t
ORIGIN      
        1 caaccgcatc gggtcgaaat tcctgcatgc cgggccgggc tatggcggct cgtgctttcc
       61 caaggatacg ctggccttcg cccggaccgg gcaggatttc ggctgcccgc tgcgccttgt
      121 cgaaacgacg gtggtggtca acgaccagcg caagcgcgcc atggctcgcc gcatcatcgc
      181 cgcctgtggc ggcaatgttc gcggccgccg gatcgccgtt ctcggcctga ccttcaagcc
      241 caacaccgac gatatgcgcg atgcgccgtc gctggccatc gtgcaggcgc tcgtcgatgc
      301 cggggcggtg gtgcgcgcct acgatccgca gggcatggag gcggccaagc cgcttttgcc
      361 gccgatcact tatgcgcagg acgcctatgc ggccgccgac gccgccgaat gcatcgtcat
      421 cgtcaccgaa tggaacgaat tccggtcgct ggatttcgag cggctgaagg ccgtgatgaa
      481 aacgccggtg ctggtcgacc ttcgcaatat ctataacccg gccgagctgg cccgctacgg
      541 cttcgcctat gaatcgatcg gtcgcggcaa ggaccgcaac ggggatcagg cgccggcccg
      601 cgtcatacgg ctttcagcct gaccggttct gctctatcgg catgggttag cggcaatact
      661 tggcggcatc gtcggcagct tgcgccggga gcaggatgct gccgctctcg ccgccctcct
      721 tctggaaatc cgccaggctg ccatatttgc ccgacggcat gatttcaggc tctatcttga
      781 agctcctgtc gcccagatcg ccgctgtagc ggttttgcct gaactcgcca taggctgagc
      841 catgacccgg cgcatagacc gccgcggcaa gtccgccgcc gccgatcgac accgtattat
      901 cctcgatccg gttgggcttt gcccatgccg gcttccagtc ccggccggtg ccgtcgtcga
      961 ggcggaaggc cgcgcccgat ccttgcgcga tgaaattgcc ggtcacgacg ttgttccagc
     1021 cgccattgat gccgatggcg gcgacgttgc cgcaaaggac gtttccttct atccgcacgc
     1081 cgcttgcctt caggtcagta tagatggccc agctgatggg cgagggccac tcgtcggtat
     1141 tctcataatc cgtgggccag aacgttccgt ccgccctgtt catcaactgg cgcgtgccgg
     1201 tgaccacatt ggcgcggatg atgctgtttt gcaggtcggc ctgcgtgccc atcagcttga
     1261 tggcgccgcc atcggcggtt tgccggttgg cgtttctcac ttcattatgt tcgatgaccg
     1321 cattgtggat ggcgtcgttg ctttcccaga tcgaaccgcc ggcgatgccg agctgggcgg
     1381 tgtcttcgac aagattgccg gcgatgcgca cattgtcggc ggcatggaac cagatgccgg
     1441 agctttcgaa atagacgcgg ccgatatcat ggatgtggtt ggccagcacc tttgcgccgc
     1501 tggatttgcc gaacttgcca tagaccgtgc cgatatagat gccgtcgccg gcgacatggc
     1561 cgatctcgtt atgggcgata aggacatttt cgctttcgga aacgtggatg ccgacgccga
     1621 cattgtcgat cacattgccg accagcctca cgccatcgga atgctccagc cggatcgcgc
     1681 cgaagccgcg catgtcggtg ccgtatttgc cggaaccctg cggcgcgcca tcgcggaatg
     1741 taaggccgga aatggtcatt ccgtcggcgt cttccagccg caggaaggta ggcaggacgc
     1801 cggcgacgat cttggcggat gtgaacgagg gcgttgccgg catgtagcta agctgtccgg
     1861 cggtggcgtc gtaccaccac tctcgcggag catcgagcag cgcgcgcgag ccggaaagga
     1921 aatagcggct tccctccgcg ctgaagaaat aggcagtgcc gccggtatgg atggtgcgcg
     1981 tgcccgcatc gatcgacagt accggaagcg tgtcgctgcc ccattggctg ccgggcagga
     2041 aaccgccgac gatatgcgcc accaatccgg ccgcgtcctt cagtgccggc aggtcgccgg
     2101 ggcggaagcg aaagcgtata ttgcccagcc aggcatcgac gtccggcttg tcggcgaata
     2161 gccagccctt gcgcgggtcg ttatcgtcgg gcgcattggg gtagcgggcc tcgtcttgta
     2221 cgatgccgtc gacgaaaagg tcacccacgc tttggccgga tggcagccgc agcggcgcaa
     2281 cccagcggcc accgccctgg gatgaccatt tcgtcaccgg cgggccgcca tgcagcaccg
     2341 gcgtttcacc gcatttggcc agcacggcca gcccggcatc ctgcgggccg aacacgacgg
     2401 ggcttgcaag ataataatcg ccgccgccca tcacgattgt tgccggtccg ccggctttgc
     2461 gcgccgaatc gcgcgccgtc tccagcgtgg cgaaggggcc gctggcgcgc tgcgcgtcag
     2521 cgagcggcgc ggcaccgctc cagcggtcgt tgccttgcgg agagacgtag aagacatgcc
     2581 gcgccgcggc cgcgcctgtt gccggaaagt aggcggccgg caagtcgcct gccttcaaat
     2641 caccagcctt gcagccagcc tgtgcaaccg gaccgacagg cgaggaaggg ccagcccgcg
     2701 ccagcgcgcc atccgatccc aacggagagg caagcagcgc gagaccgagg acaagtacga
     2761 accgcagcgt caccggattt gtagcccggc cggcaggctt gcatagccgg catgcggcgc
     2821 gggcttcttt gcatagacgg gagagtagag cgaaaacagg atgatggcgg tggcgaacag
     2881 gacgaaaata gtgtcgttct ggaccaggaa gatgctttcg gtcaggttca tcaccatgac
     2941 catcaccatg aagacgttca gccataacca gccgtcttcg ggcgcgcggc attgcaggac
     3001 ggcgccctga taggccgcgc gaatgacgac cagcatgaac aggcacatgc cgccgatgcc
     3061 gaagctgagc agcgtgtcgc gaaatccatt atgggcatgc ggcgccatcc actggatttg
     3121 cgaccagatc gaccacgctt ccgggttggc ttccgtccag aaggcctgat agccgaagcc
     3181 gagcaggaga tggcggccga tctcggcatc caccagttcc cacagcggca cccggccggt
     3241 cagcgtggcg tccttgccaa gcgcctccag caccggaacc aggaattcgt gcagcaggat
     3301 cagcagcacc gccgccatct ggacgaagag catcacgaag gcggcccggc cgatgccgcg
     3361 cgtcttcggc agcacggtgt agaaccatgt caggcagcac gccgaggccg tcgctatgat
     3421 ggacgtcatc gaggtggagc cgatcaggca ggcgatgctg gcgatcagca gtgcgatggc
     3481 cgagcggcgg tggccgtagg agccgttggt caggacgatg aagcttatca gcgtcagcac
     3541 gctcgcatac cagccaaggg tgttcttgtg gatgaagatg ccgcgcagcg ccccgtccag
     3601 cggcatgcgg gccaggccgg gcgaaatgcc gagcaggagc aggctcggca cgatgcaggc
     3661 gcccagcgtc atcatcaaca gcagcagcaa ttggcgcggc gtgaagcgca aggccagcac
     3721 ataggccaaa agcaccgcga aaagcaggcc gttcgcccgc cgcagcgtcg ttccggggct
     3781 gaccgaccac agcacggaca cgaacggcat ggcaaacagg aagtggatca gcaggtttcg
     3841 tttgagcgcg acgagaaact gccgggaatg ccgcgacagg atcagaatgg agaaggcgta
     3901 gatcggcagc gagacgaagc gcagcttggc cctggccgca tcgccaaggt cgccgttcgg
     3961 atcggccaac agcagcggga aaagcgcgcc ggtctggaga aacaggcaga tgccggcgcc
     4021 ccaccattcg atggtgcgcc agctgacggt cagctgtccc gtctcccgca cactgagcgc
     4081 tttcatggcc taggcccgcg ccggttggcg cggcggcggc gatcgcggaa aacgaggagg
     4141 aacatggcgg ctcatggtgg ctcaaatctt gttcagaact tttgcgccga agtccggtgc
     4201 tggagcccgg ctcggaagtc ggggcacgat cagccgtccg cgccggcagg gaaatgaata
     4261 caaacgacgc cgtggctacc tcaaacgcgc cggcctacgc cgaaagacgg tagccggccg
     4321 cacccttcgg aaacgccccg tcaaagtgct tatggccgcg cgc
//
LOCUS       DASYCS010000027         3194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_584034,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000027 DASYCS010000000
VERSION     DASYCS010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584034"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VGM46_01075"
     CDS             <1..249
                     /locus_tag="VGM46_01075"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014766691.1"
                     /note="FabB, beta-Ketoacyl-ACP synthase I, KASI; catalyzes
                     a condensation reaction in fatty acid biosynthesis:
                     addition of an acyl acceptor of two carbons from
                     malonyl-ACP; required for the elongation of short-chain
                     unsaturated acyl-ACP; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HEY4191213.1"
                     /translation="STKSLTGHSLGAAGVQESIYSILMMQGRFIGESAHIENLDPEFE
                     GMPIVRKRIDDARIDTVLSNSFGFGGTNATLVFQRHND"
     gene            263..1075
                     /gene="fabI"
                     /locus_tag="VGM46_01080"
     CDS             263..1075
                     /gene="fabI"
                     /locus_tag="VGM46_01080"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013527887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEY4191214.1"
                     /translation="MDGLMKGKRGLVMGVANDHSIAWGIARKLSEQGAELAFSYQGEA
                     FGRRVKPLAEQLGASLIVPCDVEDSASVAATFQTLGDAWGGLDFVVHAIGFSDKNELK
                     GLYADTSRDNFVRTMVISCYSFTEVARHAAALMKDGGSMVTLTYAGSVRVMPNYNVMG
                     VAKAGLEASVRYLANDYGPRGIRVNGISAGPVRTLAGAGIADARHMFNYQQRNSPLRR
                     TVTIDEVGGSALYLLSDLASGVTGEIHYVDSGYHIVSMPTLDALKEMDGARE"
     gene            1192..2742
                     /locus_tag="VGM46_01085"
     CDS             1192..2742
                     /locus_tag="VGM46_01085"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006337953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HEY4191215.1"
                     /translation="MSFDKNPNRTPLFRLVTIASSGFGSLLLGIWGLRFVFGDRLLDM
                     PAQTVVAIVAGLCALAAAGAALSFYAGIDESADYVYNETHFDKLTNLFTRTAMVGKIA
                     EAAARTAKTGKPVYLIDVDIDRFKQINDAIGYGQGDELIRSFTQRLRDYMPEGVAIGR
                     IGAGEFGVLYPDNAVTGTMGSIVEKLIEEMMVPYQLSTHMQSVSISVGVVAMPKDGTD
                     PTLVLRRSNLALQNARAGGVGNWSIFHSDMGKVADHRQWIEAELHPAFTRGEFDLHYQ
                     PQFDLSTGKTVGYEALIRWKHPERGMIAPMEFIPVAEETGMIMPIGEWVLRKACTDAR
                     LLPKECFVAVNISPLQFMAKDFVGTVRQVMAQTGIEPSRLELEVTETAMMQDRDRAAD
                     ILKQLCEMGISVAVDDFGTGYSNLSYLIDFSFGKLKIDRSFVSRMDTDASSGAVVSTI
                     VGLSRALGVSIIAEGVETEAQAALLRAAGCEVVQGFLYGRPAPLSLDPGTGRAEQYLP
                     EKRVASVH"
     gene            complement(2856..>3194)
                     /locus_tag="VGM46_01090"
     CDS             complement(2856..>3194)
                     /locus_tag="VGM46_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEY4191216.1"
                     /translation="HEADFDALEAAKSNIADAKIPVRFFWTDLLGEAVEQRYDAIVMN
                     PPFHRARAAEPEIGAGMVRAAAKALKPGGRLFMVANRQLPYEDTLGAVFSSFGEIARE
                     GMFKVFAARR"
BASE COUNT          606 a         1000 c          968 g          620 t
ORIGIN      
        1 tcgaccaagt cgctgaccgg ccattccctg ggcgccgccg gcgttcagga atcgatctat
       61 tccatcctga tgatgcaggg ccgcttcatc ggcgaaagcg cgcatatcga gaatctcgac
      121 cccgaattcg aaggcatgcc catcgttcgc aaacgcatcg acgatgcccg gatcgacacc
      181 gtgctgtcga actcgttcgg tttcggcggc accaacgcca cgctggtttt ccagcgacat
      241 aatgattgag ggttccacaa cgatggacgg actgatgaag ggcaagcgcg ggcttgtcat
      301 gggcgtcgcc aacgatcact cgatcgcctg gggcatcgcc aggaagctct ccgagcaagg
      361 cgcggagctt gccttcagct atcagggcga agcgttcgga cgccgggtca agccgctggc
      421 cgaacagctc ggcgcttcgc tgatcgttcc ctgcgatgtc gaggacagcg cctccgtcgc
      481 ggcgacgttc cagacccttg gcgatgcctg gggcggcctc gatttcgtcg tccacgccat
      541 cggcttttcc gacaagaacg aactcaaggg cctttacgcc gacaccagcc gcgacaattt
      601 cgtgcgcacc atggtgatct cctgctactc gttcaccgaa gtggcgcgcc atgccgccgc
      661 cctgatgaag gatggcggct cgatggtcac gctgacctat gccggatcgg tgcgggtgat
      721 gcccaactac aacgtcatgg gcgtcgccaa ggccgggctg gaagccagcg tgcgctacct
      781 cgccaatgat tacggtccgc gcggcatcag ggtcaacggc atctcggccg gtccggtgcg
      841 cacgctggcc ggcgcgggca ttgccgatgc gcgccacatg ttcaactacc agcagcgcaa
      901 ttcgccgctg cgccgcaccg tcaccatcga tgaagtcggc ggctcggcgc tgtacctgct
      961 ttccgacctg gcctcgggcg tgaccggcga aatccactat gtcgattccg gctatcacat
     1021 cgtctcgatg ccgacgctcg atgcgctgaa ggagatggac ggcgcgcggg aatagcaccc
     1081 gccgcccgct atcgccgcga cggcgttttt ccacttaaaa tttaaagcat tgtcggaaac
     1141 ccattttaag gggaattccg ctacaaggtc cccgacttgg ggacgtcgtg catgtctttc
     1201 gacaaaaatc cgaaccggac gccgcttttt cggctggtta cgatcgccag ttcgggtttc
     1261 ggcagcttgc tgcttggcat atggggcctg cggttcgttt ttggcgacag gctgctcgat
     1321 atgccggcgc agaccgttgt cgccatcgtt gccgggctgt gcgcgcttgc ggccgccggc
     1381 gcggcgctgt cgttctatgc gggcatcgat gaatcggccg attacgtcta taatgaaacg
     1441 catttcgaca agctgaccaa ccttttcacc cgcaccgcca tggtcggcaa gatcgccgaa
     1501 gcggcggcca gaaccgccaa gaccggcaag ccggtctatc ttatcgatgt cgatatcgac
     1561 cgcttcaagc agatcaacga tgccatcggc tacggccagg gcgacgaatt gatccgcagc
     1621 ttcacgcagc ggctgcgcga ttacatgccg gaaggcgtcg ccatcgggcg gatcggcgcg
     1681 ggcgaattcg gcgtgctcta tccggataat gccgtgaccg gcacgatggg atcgatcgtc
     1741 gaaaagctca tcgaagaaat gatggttccc taccagctca gcacgcatat gcagtcggtc
     1801 agcatttcgg tgggcgtcgt cgccatgccc aaggatggga ccgatccgac gctggtgctg
     1861 cgccgttcta acttggcctt gcagaatgcc cgcgccggcg gcgtcggcaa ctggtcgatc
     1921 ttccattccg acatgggcaa ggtcgccgac cacaggcagt ggatcgaagc ggaacttcat
     1981 ccggcgttca cgcggggcga gttcgatctc cattaccaac cgcaattcga cctttcgacc
     2041 ggaaaaaccg tcggttacga ggcgcttatc cgctggaaac accccgaacg cggcatgatc
     2101 gcgccgatgg agttcattcc ggtcgctgag gaaaccggca tgatcatgcc gatcggcgaa
     2161 tgggtgctgc gcaaggcctg caccgacgcc cgccttctgc ccaaggagtg cttcgtggcc
     2221 gtcaacatct cgccgctgca attcatggcc aaggatttcg tcggcaccgt gcgccaggtg
     2281 atggcgcaga ccggcatcga accgtcccgg ctggaactcg aagtgacgga aaccgcgatg
     2341 atgcaggacc gcgaccgcgc cgcggacatc ctcaagcagc tttgcgaaat gggtatttcg
     2401 gttgccgtcg acgatttcgg caccggctat tccaatttga gctacctgat cgacttctcg
     2461 ttcggcaagc tgaagatcga ccgctccttc gtcagccgga tggacaccga cgccagttcc
     2521 ggcgcggtgg tttcgaccat cgtcgggctt tcaagggcgc tcggcgtgtc gatcatcgcc
     2581 gaaggcgtcg agacggaagc gcaagcggcg ctgttgcgtg cggcaggctg cgaggtggtg
     2641 cagggctttc tctacggccg gccggcgccg ctgtcgcttg acccaggcac tggcagggcc
     2701 gagcaatatt tgccggaaaa gcgcgtagcc agcgtgcatt aacgcgcttt gtcgtccgat
     2761 tcagcgcgca aaagacgcta caatgccttg agaaagcatc ggaacagacc gaaaatcggg
     2821 gttcagcctc tttgattgat cgatgaagcg cgcattcagc ggcgcgcggc aaacaccttg
     2881 aacatccctt ccctggcgat ttcgccaaag gatgaaaaca ccgctcccag cgtatcttcg
     2941 tagggaagct ggcgattggc gaccatgaac agccgcccgc ccggcttcag cgcctttgcc
     3001 gcagcgcgca ccatgcctgc gccgatttcc ggctccgccg cgcgcgcgcg gtggaaaggc
     3061 gggttcatga cgatggcatc gtagcgctgc tcgacggctt cacccagcag atcggtccag
     3121 aagaagcgca ccgggatttt cgcatcggcg atattcgact ttgccgcttc cagcgcgtcg
     3181 aaatccgctt cgtg
//
LOCUS       DASYCS010000028        17625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4497712,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000028 DASYCS010000000
VERSION     DASYCS010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 17625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17625
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4497712"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..1182)
                     /locus_tag="VGM46_01095"
     CDS             complement(73..1182)
                     /locus_tag="VGM46_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008839576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEY4191217.1"
                     /translation="MLKRPALTAVVDALAATVPFVGPEAQQRASGRSFRARIGANESS
                     FGPSPRVIARMGEAARDMWMYCDPDNYDLKVASAAHLGISPENIVVGEGIDGLLGLLA
                     HMYVSPGTPVVTSLGAYPTFNFHIAAFGGRVVAVPYENDRESLDGLLAAVRRENAPLV
                     YLSNPDNPMGSYWDAAEIVRFIEALPETTMLVLDEAYGELGPASALPAIDVERPNLIR
                     MRTFSKAYGLAGIRCGYAFGERTVVSNFEKIRNHYGVSRMAQIAGVEALSDQAWLQSV
                     VARVGAGRSRIAAVAEANGLKPLPSATNFVTIDCGGDGDFALKIMQGFLARDVFIRKP
                     MAPVLNRCIRVSVGLDHELDIFAEELPGVLAAARR"
     gene            complement(1338..1646)
                     /locus_tag="VGM46_01100"
     CDS             complement(1338..1646)
                     /locus_tag="VGM46_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006201408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191218.1"
                     /translation="MNIKMLCMGAMALSMISGAALAGGGSGTSALDDAAMMAPFYTDS
                     SMKTMRGDDEFKAAWLALNKKDQDDMMSQCKTNTDKQHNDFCANAMKLGGANGAGSGG
                     "
     gene            complement(1826..1916)
                     /locus_tag="VGM46_01105"
     tRNA            complement(1826..1916)
                     /locus_tag="VGM46_01105"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1880..1882),aa:Ser,seq:gct)
     gene            2305..2613
                     /locus_tag="VGM46_01110"
     CDS             2305..2613
                     /locus_tag="VGM46_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191219.1"
                     /translation="MIEKSSGDDADFEDMKEFATERLYECVREVKSSPDMTHPVESVQ
                     ARRTHGRSEKPETLEDQLQKGLEDSFPASDPPAVVSTAIPGATKRLVGTDEVLRRRKG
                     "
     gene            complement(2855..3055)
                     /locus_tag="VGM46_01115"
     CDS             complement(2855..3055)
                     /locus_tag="VGM46_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191220.1"
                     /translation="MQSPGLILKVILAFIGCVAGSYVGDELLGGGALGWMAGGAIIAG
                     CVYPLFKTLLAWREERRRSGNG"
     gene            complement(3496..3702)
                     /locus_tag="VGM46_01120"
     CDS             complement(3496..3702)
                     /locus_tag="VGM46_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019863018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HEY4191221.1"
                     /translation="MADKLKTGDIVQLKSGGPKMTIADASAASGSILCHWFNRDGDAW
                     NPQHAGFKSDQLVRLEDPSTEAGI"
     gene            complement(4016..4912)
                     /locus_tag="VGM46_01125"
     CDS             complement(4016..4912)
                     /locus_tag="VGM46_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY4191222.1"
                     /translation="MTARREGFCDFVYAAADGLELHARLYGETNGEHWPIVCLPGLTR
                     NARDFHELALYLSQRAATARQVIAFDYRGRGRSANDPDVKNYNVVTEAGDVLAGLDAL
                     GVEAAGFIGTSRGGLIIHVLGAMRPAMLKAIVLNDIGPVIEAAGLAHIKTYLDAAPKP
                     KPFAQVAADQATVHGRDFPSLTNADWERMAHALYRETDKGWLPDFDPKLTETLANLDF
                     SVPLPTLWPQFDALAGIPMLTIRGANSRLLSQETLDEMTLRHPGMETITVAGQGHAPF
                     LETGDLPTSISRFFDQQDSSST"
     gene            complement(4909..6927)
                     /locus_tag="VGM46_01130"
     CDS             complement(4909..6927)
                     /locus_tag="VGM46_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010915090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY4191223.1"
                     /translation="MTAGRPYRSIVFGVILALLPSATLDAGLDTPPVSTVPLVRPKMA
                     APAIDALKGGLDALAANDIDRARAVRDRLPADSLDRHVLGWAIAMFGGDKAPSSEIAA
                     TAKMLPNWPGILQLRRNGERALARENPAPQAVIQAFDRAQPLTLEGTVLLARAYVATG
                     NLPAAQAVLSPFWRTEKLGAMDEAAIIAEFGAIIPIADHRFRMERMFYADRVNPALRV
                     APLAGAQELADAWAAVIRGDKAAPDLLKAVPAAQRSAGYLFAQAEYLRRRKQYGDAAA
                     IVAKAPTDAAALVDPDAWWVERRVLSRELVDHGDMKTAYAIVAGHRAESPANTAEAEF
                     HAGWYALRGLNDPKKAALHFSRIAALAEGPISLSRAYYWLGRAAETGGPGDAKAYFAK
                     AAGYGTTFYGQLAGERIGRQALTLIYPEPTIADRQAFAGREAVSAIERLQEAGYGRFA
                     DALYRDLAAQLTSPGELSLLAAMAQSERNDFLALRVVKIASARGIDVGALSHPIGVIP
                     DSADISGSGKALAYAIARQESEFNIGAVSGAGALGLLQLMPATAKQLARKSGVPFSQA
                     RLTTDAGYNAMLGAAFLGEQLGRFNGSYVLTFAGYNAGPTRAAQWVARYGDPRGKELD
                     EVVDWIERIPYTETRSYVQRVMENYEVYKMRLTGKYDIAGDLVNGRGQ"
     gene            7161..8042
                     /gene="dapA"
                     /locus_tag="VGM46_01135"
     CDS             7161..8042
                     /gene="dapA"
                     /locus_tag="VGM46_01135"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006167245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEY4191224.1"
                     /translation="MLRGSLTALVTPFDKDGRFDEKAFRAFVEWQIAEGTTGLVPVGT
                     TGESPTLSHDEHRQVVKVCVEMANGRVPVVAGAGSNNTQEAIGLAKYAESVGADAVLV
                     VTPYYNKPTQRGLYAHFAAVAGATSLPVIIYNIPPRSVIDMTPETMGRLAHDFPNIAG
                     VKDATGKVERVSEQRATCGKDFIQLSGEDASALGFNAHGGVGCISVTANIAPRLCAEF
                     QAATLANNKDLALELQDRLLPLHKAIFTEPGVSGAKYALSRLGKIENVTRSPLMTVEA
                     ATAEKLDAAMKHAGLIN"
     gene            8068..8547
                     /gene="smpB"
                     /locus_tag="VGM46_01140"
     CDS             8068..8547
                     /gene="smpB"
                     /locus_tag="VGM46_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEY4191225.1"
                     /translation="MNQVRKADPNSKVAAENRKARFNYEVLDTIETGIVLSGTEVKSL
                     RGGHANIQESYASFEGGEIWLINSYLPEYLQANRFNHEPRRRRKLLVSKREMARLSQS
                     IDREGMTLVPLKVYFNDRGRAKLLLAVARGKKLHDKRETEKQRDWNREKGRLLKERG"
     gene            complement(8663..9313)
                     /locus_tag="VGM46_01145"
     CDS             complement(8663..9313)
                     /locus_tag="VGM46_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEY4191226.1"
                     /translation="MNFAPEPDRDCPLCPRLHDFIALWREREPSWFNAPVPAFLPPEG
                     DDSVRLLIVGLAPGLRGANRTGRPFTGDYAGDLLYSTLISHGFARGRFEARPDDSLEL
                     TGTAIVNAVRCVPPENKPVGAEIATCRTFLVPTLARFRKLEAILTLGTIAHQSTVRAM
                     GERVAAHPFKHGGQSRIGDVTLFSSYHCSRYNTNTGVLTTEMFVNVFSEIRRFLDR"
     gene            complement(9323..9904)
                     /locus_tag="VGM46_01150"
     CDS             complement(9323..9904)
                     /locus_tag="VGM46_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010915096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEY4191227.1"
                     /translation="MFDAREKIALFIDGANLYATSRALGFDIDYRKLLASFQRRGYLL
                     RAYYYTALVEDQEYSSIRPLIDWLDYNGFKVVTKPAKEFTDSTGRRKIKGNMDIELTV
                     DALELVNKVDHYVIFSGDGDFRTLVEALQRQGCKVSVVSTMASQPPMISDDLRRQADH
                     FIDLTTLKAEIGRDPSERPIRRPDPVADEEDDY"
     gene            10140..10541
                     /gene="rpoZ"
                     /locus_tag="VGM46_01155"
     CDS             10140..10541
                     /gene="rpoZ"
                     /locus_tag="VGM46_01155"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015915452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEY4191228.1"
                     /translation="MARVTVEDCIDKVDNRFELVLLAGHRARQISQGAPITVARDNDK
                     NPVVALREIADETLSPDDLKEDLIHSLQKHVEVDEPEADGEVLADQTTGAVASNDADD
                     AEENITFDRMTEEDLLAGIEGLVAPEKSDDF"
     gene            10653..12890
                     /locus_tag="VGM46_01160"
     CDS             10653..12890
                     /locus_tag="VGM46_01160"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019444226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEY4191229.1"
                     /translation="MMRQYELVERVQRYKPDVNEALLNKAYVYAMQKHGFQKRASGDP
                     YFSHPLEVAAILTDMHMDEATIAVALLHDTIEDTTATRAEIDELFGPEMGKLVEGLTK
                     LKKLDLVSKKAEQAENLRKLLLAISEDVRVLLVKLADRLHNMRTLHHVPEAKRLRISE
                     ETMEIYAPLAGRMGMQGMREELEELAFRYINPEAYRMVTARLAEFFERNEHVLGEIEQ
                     SLSGLFVKYTIEAEVKSRQKKPWSVFRKMEAKALSFEQLSDIFGFRVVVGSVEDCYRA
                     LCAIHTTWSMVPGRFKDYISTPKQNDYRSIHTTIVGPSRQRVELQIRTREMNRVAEYG
                     VAAHSVYKDFGSKEGNGKADGAHTLSKDTNAYAWLRRTIEQLAEGDNPEDFLENTRLE
                     LFQDQVFCFTPKGMLIALPRGATPIDFAYAVHTDVGDTCVGAKVNGRVMPLMTELKNG
                     DEIEIIRSKAQVPPAAWESIVVTGKARSAIRRATKNAIRKQYSGLGARILERAFQRAG
                     KTFVKENLKPVLHRLARKDIEDVMSSVGRGELASSDVMKAVFPDYKDERVTVAPKQRE
                     EGWSRIRNAAGMLFHIPGRSRRDKTMRPEDSAVPIRGVRGDLPVHFAPEGAVPGDRIV
                     GIMQPGSGITIYPIQSPSLTAFDDQTERWIDVRWDIDENNKERFPARVSVTAINAPGS
                     LADIAQVVASNDANIHTLSMIRTAPDFTEMLIDLEVWDLKHLNRLLSQLKDNASVSEA
                     RRVNG"
     gene            12950..13528
                     /gene="pyrE"
                     /locus_tag="VGM46_01165"
     CDS             12950..13528
                     /gene="pyrE"
                     /locus_tag="VGM46_01165"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004691929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEY4191230.1"
                     /translation="MNTAEVLDIFREAGAVLEGHFILTSGLRSPIFLQKARVFMHADK
                     TERLCRALAAKIREKVPGKIDYVVGPAIGGLIPAYETSRHLGVPAIWVEREQGAFRLR
                     RFEIEKGARVVIVEDIVTTGLSIRETIDCLRQLGAEVVAAACIIDRSAGKTDVGVPLI
                     ALAEYEVPAYPADNLPPELAALPAVKPGSRNI"
     gene            13567..14166
                     /locus_tag="VGM46_01170"
     CDS             13567..14166
                     /locus_tag="VGM46_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019857197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HEY4191231.1"
                     /translation="MLFKRRIAESYWDRFRVWLWPRRSFSRSLQYFSKRILRLRATPH
                     AVAAGVAAGVFASFFPLGLHFIMAALVCWLIAGNLVAALLGAAVGGNPLTVPILWGAS
                     WETGKLILHGHLANHGSPGHLGEMLRDMSFNQLWGPVLKPLAIGAIPLGLVFGVLFYG
                     VTRWAMATFQERRRKRLAEKAARQLSLADKADGVGSALR"
     gene            14163..14576
                     /gene="acpS"
                     /locus_tag="VGM46_01175"
     CDS             14163..14576
                     /gene="acpS"
                     /locus_tag="VGM46_01175"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006331219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEY4191232.1"
                     /translation="MIIGIGSDLIDIRRIEKSLERYGERFIQRIYTDIEQQRSEGRRA
                     RAASYAKRFAAKEACAKALGTGLSQGVFWRDMGVANLPGGKPTMQLSGAAAERLAKML
                     PAGHRAEIHLTITDDYPLAQAFVIIEALPAEIPPH"
     gene            14644..15393
                     /gene="lepB"
                     /locus_tag="VGM46_01180"
     CDS             14644..15393
                     /gene="lepB"
                     /locus_tag="VGM46_01180"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEY4191233.1"
                     /translation="MSVAEKPQKKAGGLGETVSVIVQALLLALVIRSLFFQPFSIPSG
                     SMRPTLLEGDYLFVTKWAYGYSRYSLPFSPNIFSGRIWGSEPKRGDVVVFKFPPDPSV
                     DYIKRVIGLPGDKIQMKDGQLFINGVGVPREKVGQIDNPDVTEVDRPVDVYRETMPNG
                     VSYDTLDLTQNGLGDNTREFDVPPGHYFMMGDNRDNSSDSRFTVGYVPDENLVGRANI
                     IFFSIAGGASPLEIWKWPTDMRVSRLFHFIN"
     gene            15393..16109
                     /gene="rnc"
                     /locus_tag="VGM46_01185"
     CDS             15393..16109
                     /gene="rnc"
                     /locus_tag="VGM46_01185"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006331221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEY4191234.1"
                     /translation="MGLNRPKGDALAKALADRTGYAFKDLRLLETALTHSSAVRAVAN
                     NERLEFLGDRVLGLIVADMLFRRFPEAREGEIAPRFNALVDARTCAEIGSELQLADFV
                     RADAALKARTRGSGANYLADAVEALIAAIYIDGGIDAARGFVLRYWEPRLSMVIAKPA
                     NPKSELQEWVARKSDARPEYAIEKREGPDHQPVFTVSVTVTGFAPSLGAGGSRRVAEE
                     AAATSFLVREGVWTDEGNAE"
     gene            16106..17032
                     /gene="era"
                     /locus_tag="VGM46_01190"
     CDS             16106..17032
                     /gene="era"
                     /locus_tag="VGM46_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEY4191235.1"
                     /translation="MTGPDDTPAAQPATRSGFAALIGAPNAGKSTLLNQLVGAKVSIV
                     THKVQTTRALVRGIATHENAQIVFVDTPGIFKPKRRLDTAMVTTAWGGARDADIVLVL
                     IDAERGIRGDANAILERLKDVRQPMVLVLNKVDRVKHETLFALAEAANERVPFKRTFM
                     VSALTGSGCKDLLDYLAEALPAGPWYYPEDQISDLPMRQLAAEITREKLYLRLHQELP
                     YSSHVETERWEEKKDGSVRIEQVIYVERDSQKKIVLGHKGETIRAIGQSARMEIAGIL
                     EQKVHLFLFVKVRENWGDDPERYREMGLDFPH"
     gene            17447..>17625
                     /locus_tag="VGM46_01195"
     CDS             17447..>17625
                     /locus_tag="VGM46_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEY4191236.1"
                     /translation="MNIKSLLLGSAAALVAVSGAHAADAVVVAEPEPAEYVRICDVYG
                     AGYFYIPGTETCLRVG"
BASE COUNT         3473 a         5452 c         5451 g         3249 t
ORIGIN      
        1 cttttgcgtc cgaaaatgcg gccaaaccaa ggatagagcg gttttaccga ttcggtaaaa
       61 accagaaccg ctctagcgcc gcgcggccgc cagcacgccg ggcaattcct cggcaaaaat
      121 gtcgagttca tggtcgaggc cgacgctgac ccggatgcat cggttcagca ccggcgccat
      181 cggcttgcgg atgaatacgt cgcgcgccag aaatccctgc atgatcttga gcgcgaagtc
      241 gccgtcgcca ccgcaatcga tggtgacgaa gttggtcgcc gaaggcagcg gcttcagccc
      301 gttcgcctcg gccacggcgg ctattctgga gcggccggcg ccgacgcgcg ctaccacgct
      361 ttgcagccag gcctgatcgg acagcgcctc gaccccggcg atctgcgcca tgcggctgac
      421 gccgtaatgg ttgcgtatct tctcgaagtt cgaaacgacc gtccgctcgc caaatgcata
      481 gccgcagcga atgcccgcca aaccataggc tttcgagaag gtgcgcatgc ggatgaggtt
      541 agggcgctcc acgtcgattg ccggcagcgc cgatgcaggc cccaattcgc cataggcttc
      601 gtcgagaacc agcatggtgg tttcgggcag cgcctcgatg aagcgcacaa tttcagcggc
      661 gtcccaatag cttcccatcg gattgtcggg attggaaaga tagaccagcg gcgcgttttc
      721 ccggcggaca gcggcaagca ggccatcgag gctttcgcgg tcgttttcat aaggcaccgc
      781 caccacgcgg ccgccaaacg cggcgatgtg gaaattgaag gtcggataag cgccgagcga
      841 ggttacgacc ggagtacccg gcgagacata catatgggca agcagcccga gcagtccgtc
      901 tatgccttcg ccgaccacga tgttttccgg cgaaatgccg agatgcgcgg cggaggcgac
      961 cttcaggtcg taattgtcgg gatcgcaata catccacatg tcgcgggccg cctcgcccat
     1021 gcgggcgatg acgcgcgggg agggaccgaa actgctctca ttggcgccga tgcgggcgcg
     1081 aaaagaacgc ccgcttgccc gctgctgcgc ttccgggccg acaaaaggca cggtggcggc
     1141 aagggcgtcg acaaccgcgg tgagggcagg gcgtttcagc attgcgaagg ctcggttgag
     1201 aaggccttct agctacagcg gctggccggc ccgcacaagg catgaaccgg ggagcgggtc
     1261 cacgcgccgg aaaagaaaaa tggcgggcaa attgcccgcc atgttccgca aaacacaatc
     1321 tgtgtttgct gaaagtatca gccgcccgaa ccagcgccat tcgcgccgcc gagcttcatg
     1381 gcgttggcgc agaagtcgtt gtgctgcttg tcggtgttgg tcttgcactg cgacatcata
     1441 tcgtcctggt ccttcttgtt cagggccagc cacgctgcct tgaactcgtc gtcgccacgc
     1501 atcgtcttca tggaggagtc cgtgtagaac ggagccatca tcgcggcatc gtccagtgcc
     1561 gacgtgcccg agccgccgcc tgccaaagca gcgccggaga tcatggatag ggccatagcg
     1621 cccatgcaaa gcatcttgat attcatgaca aaaacctttc gtgttgctct tcagatcgtc
     1681 cagcgtcgat cgactggaga acgaacgatc tagggaaaag ttccaccctc tgcaagagag
     1741 agtcgcggcg gctccggcct ggatcaaaga agacttcgcg gggggatata ggtggcccaa
     1801 ctaatagtga gaggcagcaa accgatggcg gagaggatgg gattcgaacc cacgagaagc
     1861 ttttgacctc tactccctta gcaggggagc gccttcgacc actcggccac ctctccgttg
     1921 cgccgctgga tagagaaaag ccgcgccaca atcaatgacc ctgcgcgttt attaatcgga
     1981 atcacccaaa aatcattgcc ggatcgtgac ttttcgcgcc gacccgaacc gccgtccgcc
     2041 ttcgtgccgt ggtgatcgcg cgatctgaaa attcatcggc gatggccgga accggcatga
     2101 cttcggcgcg ttgaaaattc atgcgattgg acccggagtg acgatatgtc gaagttacga
     2161 cacggctcaa gcgacgagct tgacagcata cgtctaaagg caatttctta tttcgcgccg
     2221 atgatgctgc atgaggcatt gcttcgcctg tgcagggcgg gcgggggaga aagcattgat
     2281 cgtttcgaaa aaacgatgat tgcaatgatc gaaaaatcct cgggagacga tgcggatttc
     2341 gaggacatga aggaattcgc gaccgagcgg ctctacgaat gtgtccgcga ggtgaaatct
     2401 tcgcccgata tgacgcatcc cgtcgaaagc gttcaggcgc gaagaacgca tggccgctcg
     2461 gaaaaacccg aaaccttgga agatcagctt caaaaaggcc tcgaagacag ctttccggcg
     2521 agcgatccgc ccgccgtcgt ctcgaccgct attcccggcg ccacaaaacg cctggtcggc
     2581 acggatgaag tcttgaggcg aaggaagggc tagctggagc gtggccggcc ccggtttttg
     2641 aacctgtccg gtttttatcc ggtgtatcgc cggcatatat gctgcgcgcc ggacgggaca
     2701 ggtgctcgtc tgccatttcg ttggccagga ggggaaaccg ggaaaatcct cccgttcacc
     2761 gtaccgtcag gcccggttga cacggcccgc agccatccac aacgcttgac ccgccaaaag
     2821 gttctgtcca cggggtgacc gggccagaaa gtcttcagcc attgccggag cgccggcgct
     2881 cctcgcgcca tgcaagcaat gtcttgaaca ggggatacac gcaaccggcg atgattgcgc
     2941 ctcccgccat ccagcccagc gcgccgccgc cgagcagttc gtcgccgaca tagcttccgg
     3001 caacgcaacc gatgaaggcc agtataacct ttaggatcag gcccggtgac tgcatgccga
     3061 ttctcctcgc gctaggcagg cactaaagca tcaagccgct ttgcgggcaa tccggctgcc
     3121 gaagctgccg gtggagcaat ccgtcaccat ccttgacgcc gtaaagctcg ctcaacgatc
     3181 ggcgtgaaaa acacaggtga tgcagccacg ggggtcatcc ttcgcggcca tggaggcagc
     3241 gatatatccg cagatcagcg acatgatcag ggcgataagg accgggatat gatgcatgcg
     3301 gagttgtagc ggattgccgc gccaaaccac gaggcaggtt cggcaacggt ccaccggaga
     3361 ccggcaacgt ggtaaatatc tggtaaaaaa acgagtgcgt ggtgtagcgg catcggcgca
     3421 ttggcgctgc gggaactgaa ccggccgctg cgacgcggcg atattggcgc gatgcccgac
     3481 gcccgttcgg ccaggctaga ttccagcctc ggtggagggg tcttcgagcc tgacgagctg
     3541 gtcggacttg aagccggcat gctgcgggtt ccaggcatcg ccatcccggt tgaaccaatg
     3601 gcaaaggatc gatcccgagg ccgccgatgc gtcggcgatg gtcattttgg ggccgcccga
     3661 tttcaattgg actatatcac cggtcttaag cttgtccgcc atgatccatc ctcccttttg
     3721 atcgaaacgt cagctaatca tcaagccgga gccacggcaa tgcctcagca tcggcgacgc
     3781 acaggcggcg cagcgaggcg tgggatcatt cttgtctgga tatcagccaa agtcgctcgg
     3841 ccttcagggc agcgccgctt gggggcgcga tatcgagatg atcgagatat gatttcgagg
     3901 ttggccggcc acaaaacgtc gtgccctccc ggctttcccg caaaggcgcg ccggccgctc
     3961 gatcgcagcc cggccatcgg caaccgccat ctccttgggt agaaaccacc atatcctagg
     4021 tggaagatga gtcctgttgg tcgaagaagc gggagatcga tgtcggcagg tcgccggttt
     4081 cgaggaacgg cgcatggcct tggcccgcca ccgtgatcgt ttccatgccg ggatggcgca
     4141 gcgtcatctc gtccagcgtt tcttgcgaaa gcagccgtga attggccccg cgtatcgtca
     4201 gcatgggaat gccggcaagc gcgtcgaatt gcggccacag cgtcggcaaa ggcaccgaaa
     4261 agtcgagatt tgccagcgtt tcggtcagtt tcgggtcgaa atccggcaac cagcccttgt
     4321 ccgtttcgcg gtagagcgcg tgcgccatgc gctcccagtc ggcattggtc agcgacggaa
     4381 aatcccggcc atgcacggtg gcctggtcgg ctgcgacctg ggcgaagggt tttggcttcg
     4441 gggcggcatc gagataggtt tttatgtggg caagccccgc cgcttcgatc accgggccga
     4501 tatcgttgag gacgatggcc ttgagcatgg ccgggcgcat cgcccccaga acatggatga
     4561 tgaggccacc gcgcgacgta ccgatgaatc cggccgcctc gacgccgagc gcatccagcc
     4621 ctgccagcac gtcgccggct tcggtgacga cattgtagtt cttgacgtca ggatcattag
     4681 ccgagcgtcc gcgcccgcga taatcgaagg cgattacctg ccgcgcagtt gccgcgcgct
     4741 gcgacagata aagcgccagt tcgtgaaaat cgcgcgcatt gcgggtcagg ccgggcaggc
     4801 agacgatagg ccaatgctcg ccattcgttt cgccgtaaag gcgggcatga agttcaagcc
     4861 cgtcggcggc ggcatagacg aaatcgcaaa acccctcgcg ccgcgccgtc attggccgcg
     4921 tccgttcacc aggtcgccgg cgatgtcgta tttgccggtc aggcgcatct tgtagacctc
     4981 gtagttttcc atcacgcgct gcacataact gcgcgtttcg gtatagggaa tccgctcgat
     5041 ccagtcgacg acctcgtcca gttccttgcc gcgcggatcg ccatagcgcg ccacccactg
     5101 cgccgcgcgg gtcgggccgg cattgtaccc ggcgaaggtc agcacataag agccgttgaa
     5161 gcggcctagc tgttcaccga ggaaggcggc gcccagcatc gcattatagc cggcgtccgt
     5221 ggtcaggcgc gcctgcgaaa acggcacgcc cgatttcctg gccagttgct tcgccgtggc
     5281 cggcatcagt tgcaaaagcc cgagcgcgcc ggcccccgaa accgcgccga tgttgaattc
     5341 gctttcctgc cgcgcgatcg cgtaagccag cgccttgccg gaaccggaaa tatcggccga
     5401 atcggggatc acgccgatcg gatgcgacag cgcgccgaca tcgatgccgc gcgccgaggc
     5461 tatcttgacc accctcagcg ctagaaaatc attgcgctcg ctttgcgcca tggcggccag
     5521 cagcgaaagc tcgccggggc tggtcaactg cgcggcaagg tcgcggtaga gcgcgtcggc
     5581 aaagcgccca tagcccgcct cttgcagccg ctcaatggcg ctgacggcct cgcgccccgc
     5641 gaaagcctgc cggtccgcta tcgttggctc aggataaatg agggtgagcg cctggcggcc
     5701 aattcgctcg ccggccagtt ggccgtagaa ggtggtgcca tagcccgcgg ccttggcgaa
     5761 atacgccttg gcgtcgccgg ggccgcccgt ctcggcggcg cgcccaagcc agtaataggc
     5821 ccgcgacagc gaaatcggcc cttccgccag cgcggcgatg cgcgaaaaat gcagcgccgc
     5881 ctttttcgga tcgttcagtc cgcgcagcgc gtaccagccc gcatgaaact cggcttccgc
     5941 tgtgttggcc gggctttccg ctctatgccc cgccacgatg gcgtaggccg ttttcatgtc
     6001 gccatggtcg acaagctcgc gcgaaagcac gcgtcgctcc acccaccagg catcggggtc
     6061 caccagcgcg gcggcatcgg tgggagcctt ggcgacgatc gcggccgcgt cgccatattg
     6121 cttgcgccgg cgcagatatt cggcctgcgc aaacaaataa ccggccgagc gttgcgccgc
     6181 aggcaccgcc ttcagcaggt cgggcgcggc cttgtcgccc cggatgacgg ctgcccacgc
     6241 atcggccaat tcctgcgcgc cggcaagcgg ggcgacgcgc agcgccgggt tgacccggtc
     6301 ggcatagaac atgcgctcca tgcgaaaccg atggtcggca atcgggatga tcgcgccgaa
     6361 ttccgcgata atggcggcct cgtccatggc cccgagcttt tccgttcgcc agaacggcga
     6421 caggacagct tgcgcggccg gcaaattgcc cgtcgccaca taggcgcggg caagcaacac
     6481 cgtgccttcc agggtcaatg gctgggcgcg gtcgaacgcc tggatcactg cctgcggcgc
     6541 cgggttttca cgcgcgagcg cgcgctcgcc attgcggcga agctggagga tgcccggcca
     6601 gttgggcaac attttcgcgg tggccgctat ctcgctgctc ggcgccttgt cgccgccgaa
     6661 catcgctatc gcccaaccga gcacatgccg gtcgagcgaa tccgcgggca ggcgatcgcg
     6721 cacggcgcgg gcacggtcaa tatcgttggc ggccagcgcg tcgagaccgc ctttcaaggc
     6781 gtcgatggcg ggcgcagcca tctttgggcg aacgagggga acggtcgaca cgggcggcgt
     6841 gtcgaggccg gcgtcaagcg tcgcactggg caacagcgcg agaatcacac caaaaacgat
     6901 tgatcgatat ggccgtccgg cggtcatcgt ggtcgtggtt cctgccttga cccgctacag
     6961 ataggtccaa cacctaattc ctgcttgtga aaagccggtg aaatcgaatt taaatgcgcc
     7021 tgtggccccg ctgccggccg tcatctgttt gttgccccgc aacgatgtcg agactatggt
     7081 gcgccgccgc gctttgggct agaagccggg cggcattgga aaaagcaagg aaacctgaag
     7141 gtttcgggga gtttgacgac atgctgagag gctcgcttac cgcgctcgtg acaccgttcg
     7201 acaaggacgg gcgtttcgac gagaaagcgt ttcgcgcatt cgtcgaatgg cagatcgccg
     7261 agggcacgac cgggcttgtc cccgtcggca cgaccggcga gtcgcccacc ctgtcgcatg
     7321 acgagcatcg ccaggtggtc aaggtctgcg tcgagatggc caacggccgc gttcccgtcg
     7381 tcgccggcgc cggttccaac aatacgcagg aagccatagg cctggcgaaa tatgccgaaa
     7441 gcgtcggcgc cgacgccgtt ttggtggtga cgccctatta caacaagccg acgcagcgcg
     7501 ggctttacgc gcatttcgcc gccgttgccg gcgcgacctc gctgcctgtc atcatctaca
     7561 acatcccgcc gcgttcggtc atcgacatga cgcccgagac gatggggcgg ctggcgcatg
     7621 attttcccaa catcgccggc gtcaaagacg ccactggcaa ggtggagcgt gtttccgaac
     7681 agcgcgccac ttgcggcaag gatttcatcc agctttcggg cgaagacgct tccgcgctcg
     7741 gcttcaacgc gcatggcggg gtagggtgca tttccgtgac ggccaatatc gcgccgcgcc
     7801 tgtgtgccga gttccaggcc gcgacgctgg ccaacaacaa ggatctggcg ctggagttgc
     7861 aggaccggct gttgccgctg cataaggcga tcttcaccga acccggcgtt tcaggcgcga
     7921 aatacgccct gtccaggctc ggcaagatcg aaaacgtgac gcgttcgccg ctgatgaccg
     7981 tcgaagccgc gactgcggaa aagctcgatg cggccatgaa gcacgccggc ttgataaatt
     8041 gagggcgagg ggccacctct ccacatcatg aaccaggtca gaaaagccga tcccaacagc
     8101 aaggtcgccg cggaaaaccg caaggcgcgc ttcaattatg aagtgctcga caccatcgag
     8161 accggcatcg ttttgagcgg caccgaggtc aagtcgttgc gcggcggcca tgccaacatc
     8221 caggaatcct acgcttcctt cgagggtggc gagatatggc tgatcaattc ctacctgccg
     8281 gagtatctgc aagccaaccg cttcaatcat gaaccgcgca ggcgtcgtaa gctgctggtg
     8341 tcgaagcgcg aaatggcccg cttgtcgcaa agcatcgacc gcgaaggcat gacgctggtg
     8401 ccgctcaaag tttatttcaa cgaccgtggc cgcgccaagc tgttgctggc ggtggcgcgc
     8461 ggcaagaagc ttcacgacaa gcgcgaaacc gaaaagcagc gcgactggaa ccgcgaaaag
     8521 ggccgcctgc tgaaggaacg cgggtaaggc gcgatcggcc agaggtcgag gaaaagccag
     8581 tgatttggct tttcggcgac gaacgcccgg tgccacagcg aaggccgggc cgccatgatc
     8641 gaacgcttcg ccgcttgcga atctatctat ctagaaatcg ccgtatctca ctgaaaacgt
     8701 tcacgaacat ttccgtggtc aatacgcccg tattggtgtt gtagcgcgag cagtggtagc
     8761 tggaaaacag cgtcacatcg ccgatcctgc tttggccgcc atgcttgaac ggatgcgccg
     8821 ccacgcgctc tcccattgcc cgcacggtcg attgatgcgc gatggtgccg agcgtcagga
     8881 tcgcttccag cttcctgaac cgcgccagcg tcgggacaag gaaggtgcgg caggtggcga
     8941 tctccgcgcc gaccggcttg ttttccggcg gcacgcatcg cacggcattg acgatggcgg
     9001 tgcccgtcag ttcaaggctg tcgtctggcc tggcctcgaa gcggccgcgc gcaaatccgt
     9061 gcgaaatcag cgtggaatag agcaggtcgc cggcatagtc gcctgtaaag gggcgcccgg
     9121 tgcggttggc gccgcgcagg ccgggcgcca gtccgacgat cagcaggcgt acgctgtcat
     9181 cgccctccgg cggcaggaaa gccggcacgg gcgcgttgaa ccaggaaggc tcgcgctcgc
     9241 gccacaacgc gatgaaatca tgcagccgcg ggcaaagcgg gcagtcgcgg tcaggctcag
     9301 gggcgaagtt catgcgcaag gctcagtaat cgtcttcctc gtcggcgacc gggtctggcc
     9361 ggcggatcgg acgttccgaa ggatcgcggc ctatttcggc cttgagcgtt gtcaggtcga
     9421 tgaagtggtc ggcctggcgg cgcagatcgt cggaaatcat cggcggctgc gaggccatgg
     9481 tcgaaacgac cgaaaccttg catccctgcc gctgcaaggc ttccaccagc gtgcggaaat
     9541 cgccgtcgcc tgagaatatg acgtaatgat cgaccttgtt gaccagttcg agcgcatcga
     9601 ccgtcagttc gatgtccata ttgcccttga tcttgcggcg tccggtcgag tcggtgaatt
     9661 ccttggccgg cttggtgacg accttgaaac cattatagtc gagccagtcg atgagcgggc
     9721 ggatcgagga atattcctga tcctccacca gcgcggtgta gtaataggcg cgcagcaggt
     9781 agccgcgcct ctggaagctg gccaagagtt tgcgatagtc gatatcgaag cccagcgcgc
     9841 gcgatgttgc atataaattg gccccatcta tgaaaagcgc tatcttttcg cgggcatcga
     9901 acatgaaaaa tatccttttt tgcaatgcgc aacaattgat aggatcgata ctggcacagt
     9961 atcgtaaaat tccggctcaa aactcatttt aagaaatagc ggcggctttg tgactatcca
    10021 gcctcgttcc gtcaatatgt aggcaatttg acggaaagcc gcgcgcttgt attttcgcgg
    10081 cgttcgggtt atggaccgca cctgttttcc gtcacatcca tactatgaaa ggggcattca
    10141 tggcccgcgt aaccgttgaa gattgcatcg acaaggtcga taaccgcttc gaactggtgc
    10201 ttttggccgg tcaccgcgcc cgccagatca gccagggcgc gccgatcacc gtcgctcgtg
    10261 acaacgacaa gaaccccgtc gtcgccttgc gcgaaatcgc cgacgagacg ctgtcgcccg
    10321 acgatttgaa ggaagatttg atccattcgc tgcaaaagca tgtcgaagtc gacgagccgg
    10381 aggccgatgg cgaggttctt gccgatcaga ccacgggcgc tgtcgcttcg aacgatgccg
    10441 acgatgcgga agagaacatc accttcgacc gcatgaccga ggaagatttg cttgccggca
    10501 tcgaaggcct ggtcgcgccg gagaaaagcg acgatttctg accatttgag cggtaaacgg
    10561 ccctttcgcg ccggaacgtt catagctatt tatgacatgc gccgcatatg cttgcggcgc
    10621 atgtttcatt ttcagcgttt gagagcctgc ccatgatgcg tcagtacgag cttgtcgagc
    10681 gcgtccagcg ctacaagccc gacgtaaacg aggcgctgct caacaaggcc tatgtctacg
    10741 ccatgcagaa gcatggcttc cagaagcgcg cctcgggcga tccctatttc tcgcatccgc
    10801 tggaagtggc ggcgatcctc accgacatgc acatggacga ggccaccatc gcggtcgccc
    10861 tcctgcacga caccatcgag gacaccaccg ccacccgcgc cgagatcgac gaactgtttg
    10921 gcccggaaat gggaaagctg gtcgagggcc tgaccaagct gaagaagctc gacctcgttt
    10981 cgaaaaaggc cgagcaggcg gaaaacctgc gcaagcttct gctggcgatt tccgaagatg
    11041 tccgcgtgct gctggtcaag ctggccgacc gcctgcacaa catgcgcacc cttcaccacg
    11101 ttcccgaagc caagcgcctg cgcatctccg aggagacgat ggagatttac gcgccgctgg
    11161 ccgggcgcat gggcatgcaa ggcatgcgcg aggaacttga agaactcgcc ttccgctaca
    11221 taaatccgga agcctaccgc atggtcacgg cgcggctggc cgaattcttc gagcgcaacg
    11281 agcatgtgct gggcgaaatc gagcagtcgc tgtcggggct gttcgtgaaa tacacgatcg
    11341 aggcggaggt aaagagccgc cagaagaagc cgtggtcggt gttccgcaag atggaggcca
    11401 aggcgctctc cttcgagcaa ctatccgaca ttttcggctt tcgcgtcgtg gtcggcagcg
    11461 tcgaggattg ttaccgtgcg ctttgcgcca tccataccac ctggtcgatg gtgcctgggc
    11521 gcttcaagga ttacatttcg acgcccaagc agaacgacta ccgctccatc cacaccacca
    11581 tcgtcggccc atcgcgccag cgcgtcgaat tgcagatccg cacgcgcgaa atgaacaggg
    11641 tggccgaata tggcgtcgcc gcccattccg tctacaagga tttcggctcc aaggaaggga
    11701 atggcaaggc cgatggcgcc cacaccctct ccaaggatac caacgcctac gcctggctgc
    11761 gccgcaccat cgagcaattg gcggaaggcg acaatccgga ggatttcctt gaaaataccc
    11821 gtctggaact gttccaggac caggtattct gcttcacgcc gaagggcatg ctgatcgcgc
    11881 tgccgcgcgg ggccacgccc atcgacttcg cctatgccgt ccacaccgac gttggcgaca
    11941 cctgcgtcgg cgccaaggtg aacggccgcg tcatgccgct gatgaccgag ctgaagaatg
    12001 gcgacgagat cgaaatcatc cgttccaagg cgcaggtgcc gccggcggcg tgggaatcga
    12061 tcgtcgtcac cggcaaggcg cgctcggcca tccgccgcgc caccaagaac gccatccgca
    12121 agcaatattc cgggcttggc gcgcgcatat tggagcgcgc cttccagcgc gccggcaaaa
    12181 ccttcgtcaa ggaaaacctg aagcccgtgc tgcatcgcct ggcgcgaaag gacatcgagg
    12241 acgtgatgag ttcggttgga cgcggcgaac tggcatcgag cgatgtcatg aaggcggtgt
    12301 tccccgacta taaggacgag cgcgtcaccg tcgccccgaa gcagcgcgag gaaggctggt
    12361 cgcgcatccg caacgccgcc ggcatgctgt ttcatattcc tggccggtcg cggcgcgaca
    12421 aaaccatgcg gccagaagat tcggcggtgc ctatccgtgg ggtgcgcggc gacctgcccg
    12481 tgcatttcgc cccggaaggc gccgttcccg gcgaccgtat cgtcggcatc atgcagcccg
    12541 gctccggcat caccatctat ccgatccagt cgccgtcgct gaccgccttc gacgaccaga
    12601 ccgaacgctg gatcgatgtg cgctgggaca tcgacgaaaa caacaaggaa cgttttcccg
    12661 cgcgcgtgtc ggtcaccgcc atcaatgcgc cgggttctct tgccgatatt gcgcaggttg
    12721 ttgcctccaa cgacgccaac atccatacgc tttcgatgat acggacagcg ccggacttca
    12781 ccgagatgct gatcgatctc gaagtatggg atctgaagca tctgaaccgg ctgctttcgc
    12841 agctcaagga caatgccagc gtcagcgaag cgcggcgcgt caatggttga tgcggggctg
    12901 gttcggggct gatgcgagcc aataagggcc aataaggaac gattacgcaa tgaacacggc
    12961 tgaagtgctg gacatttttc gcgaggcggg cgctgtgctg gaagggcact tcatcctgac
    13021 ttccggcctg cgaagcccga tattcctgca aaaggcgcgg gtgttcatgc atgccgacaa
    13081 gaccgagcgc ctgtgccgtg cgctggctgc aaagatacgc gaaaaggtgc cgggcaagat
    13141 cgactatgtc gtcggtccgg ccatcggcgg cctcattccc gcctatgaaa cctcgcgcca
    13201 tctcggcgtg ccggccatct gggtggagcg cgagcagggc gcgttccgcc tgcgccgctt
    13261 cgagatcgaa aagggcgcgc gcgtcgtcat cgtggaagat atcgtcacca ccggcctgtc
    13321 gatccgcgaa accatcgatt gcctgcgcca gctcggcgcg gaggtggtgg cggccgcctg
    13381 catcatcgac cgttccgccg gcaagaccga tgtcggcgtg cccttgattg ccctggccga
    13441 atatgaggtg ccggcctatc cggcggataa tcttcccccg gaactcgccg cccttccggc
    13501 ggtaaagccc ggcagccgca atatttgagg ttgtttgcgc gaaaaattag gcggtaaagt
    13561 cgagttatgt tgttcaaacg caggattgcg gaaagctatt gggatcgttt tcgcgtgtgg
    13621 ctgtggccgc gccgttcgtt ttcccgctcg ttgcaatatt tctccaagcg gattttacgg
    13681 ctgcgcgcga ccccgcatgc ggtggcggcg ggcgtcgccg ccggcgtatt cgcctcgttt
    13741 ttcccgctcg gactgcactt catcatggcc gcccttgtct gctggctgat tgccggaaac
    13801 ctggtggcgg ccctgctggg cgcggcagtc gggggaaacc cgctgacggt cccgatcctg
    13861 tggggcgcgt cgtgggagac gggcaagctc atcctgcacg gccatctggc caatcacggt
    13921 tcgccgggcc atctgggcga gatgctgcgc gacatgagtt tcaaccagct atgggggccg
    13981 gtcctgaagc cgctggccat cggcgccatc ccgctgggcc tcgtcttcgg cgttcttttc
    14041 tatggcgtca cccgctgggc gatggcgacg ttccaggagc ggcgccgcaa gcggctggcc
    14101 gaaaaggccg ccaggcagct ctcgcttgcc gacaaggcgg acggcgtcgg ttccgccctt
    14161 cgatgattat cggcataggc agcgacctga tcgatatccg ccgcatcgag aaatcgctcg
    14221 aacgctacgg cgaacgcttc atccagcgca tctataccga tatcgagcag cagcgctcgg
    14281 aaggcaggcg cgcgcgcgct gcttcctacg ccaagcggtt cgcggccaag gaagcatgcg
    14341 ccaaggcgct cggcaccggg ctgtcgcaag gcgtgttctg gcgtgacatg ggcgtcgcca
    14401 atcttcccgg cggcaagccg accatgcagc tttcgggcgc cgccgccgaa aggctggcga
    14461 aaatgctgcc cgccggccat cgcgccgaaa tccacctcac catcaccgac gattatccgc
    14521 ttgctcaagc ctttgtgatc atcgaggcgt tgcctgccga aataccgccg cactagtggc
    14581 ggcaatgacg ttgcccagcg cgtgcctagc ccctatacaa ccaacgcgta gattgaggac
    14641 gagatgagcg tggctgaaaa accccagaaa aaagccggtg ggcttggcga aaccgtcagc
    14701 gtcatcgtcc aggccctgtt gctggcattg gtgatccgct cgctgttctt ccagcctttc
    14761 tcgatcccgt ccggttcgat gcgcccgaca ttgctggaag gcgactatct gttcgtcacc
    14821 aaatgggcct atggctattc gcgctattcg ctgccctttt cgccgaatat tttttcgggc
    14881 cgcatctggg gttccgagcc gaagcgcggc gatgtggtcg tgttcaagtt cccgccggac
    14941 ccgtcggtgg attacatcaa gcgcgtcatc ggcctgcccg gcgacaagat acagatgaag
    15001 gacgggcaac tgttcatcaa cggcgtcggc gtgccgcgcg agaaagtcgg ccagatcgac
    15061 aatcccgacg tcaccgaggt cgaccgtccc gtcgacgtct accgcgaaac catgcccaac
    15121 ggcgtgtcct acgatacgct ggaccttacc cagaacgggc ttggcgacaa cacgcgcgaa
    15181 ttcgacgtgc cgcccggcca ttatttcatg atgggcgata accgcgacaa ttccagcgac
    15241 agccgcttta ccgtcggcta cgttcccgac gaaaaccttg tcgggcgcgc caatatcatt
    15301 ttcttctcga tcgccggtgg cgccagcccg ctcgaaatat ggaaatggcc gaccgacatg
    15361 cgtgtctcgc gcctgttcca cttcatcaac tgatgggctt gaaccggccc aagggtgacg
    15421 cgctggcaaa ggcgctggcg gacaggacag gttacgcgtt caaggatttg cgcctgcttg
    15481 aaacggcgct tacccattcc agcgccgtca gggcggtcgc caacaatgag cgcctggaat
    15541 tcctgggcga ccgcgtgctc ggcctgatcg tcgccgacat gctgtttcgc cggtttcccg
    15601 aggcgcgcga aggcgagatc gcgccgcgct tcaacgcgct tgtcgacgcc cgcacctgcg
    15661 ccgagatcgg ttcggaactg caactggccg atttcgtgcg cgccgacgcc gccctcaagg
    15721 cgcgcacccg cggctcgggc gcgaactatc tggccgatgc tgtcgaggcg ctgatcgccg
    15781 ccatctacat cgatggcggc atcgatgccg cgcgcggttt cgtcctgcgc tattgggaac
    15841 cgcgcctgag catggtgatc gccaagccgg cgaacccgaa atccgaattg caggaatggg
    15901 tggcgcgaaa gagcgacgct cgccccgaat acgccatcga aaagcgcgag ggaccggacc
    15961 atcagccggt attcaccgta tcggtgacgg tgaccggctt tgcgccctcg cttggcgcgg
    16021 gaggctcgcg ccgcgtcgcc gaagaagccg ccgccacctc gtttcttgtg cgcgaaggcg
    16081 tttggaccga cgaagggaat gctgaatgac cggcccggat gacacgcctg cggcgcagcc
    16141 tgcgacgcgc tctggcttcg cggccctgat cggtgcgccc aatgcgggaa aatcgacgct
    16201 tctcaaccag ctggtcggcg ccaaggtgtc gatcgtcacc cacaaggtgc agaccacgcg
    16261 ggcgctggtg cgcggcatcg ccacgcatga gaacgcgcag atcgtgtttg tcgacacgcc
    16321 cggcatcttc aagccgaagc ggcggctgga cacggcgatg gtcaccaccg cctggggcgg
    16381 cgccagggat gccgatatcg tcctggtgct gatcgatgcc gagcgcggca tccggggcga
    16441 cgccaacgcc atcctcgaac ggcttaagga tgtgcgccag ccgatggtgc tggtgctcaa
    16501 caaggtggat cgggtcaagc acgagacgct gtttgcgctg gccgaggctg caaacgagcg
    16561 cgtgccgttc aagcgcacct tcatggtgtc ggcgctgacc ggctccggct gcaaggattt
    16621 gctggactat ctggccgaag cgctgcctgc cggcccctgg tactacccgg aagaccagat
    16681 ttccgacctg cccatgcgcc agcttgccgc cgaaatcacg cgcgagaagc tctatttgcg
    16741 cctgcatcag gaactgcctt attcatccca tgtcgaaacg gaaagatggg aagagaagaa
    16801 ggacggctcg gtgcggatcg agcaggtcat ctatgtcgag cgcgacagcc agaagaagat
    16861 cgtgctcggc cacaagggcg agaccatccg cgccatcggc cagtccgccc gcatggagat
    16921 cgccggcatc cttgagcaga aggttcatct gttcctgttc gtgaaggtgc gcgagaattg
    16981 gggcgacgat cccgagcgct accgcgaaat gggcctcgac ttcccgcact gaggcgacgc
    17041 ttttttgtcg gcgcaataaa gccgcctaac atattgaacg ataaccatta tgctttgcct
    17101 gacgccgcag gtcccgctgc cgatgcttcg gcctgcgcac gctccaatct caaaatgtgg
    17161 ctaatttagc acagtcctat aacgctgttt cgccaccgcg cgaagccgcg gcgcggccgt
    17221 gtattttgtc gtccaactgt catacgcgct ccctataggc tggcctttcc cgatgcccgg
    17281 agccgctgcc gggtggaagg aaatccgttc ggtttgaacg gcctttttgg gacaaggcgg
    17341 cggggacctt tcctggcgac gacaaaggag acggcgacac gcgccgcacc cttatccgtg
    17401 cccggatcga tattattgca aacagtttta ctggagagat caggaaatga acatcaagag
    17461 ccttcttctc ggctccgctg cggcgctcgt cgcagtttcc ggcgcgcatg ccgctgacgc
    17521 ggtcgtcgtc gccgaacccg aaccggctga atatgtgcgc atctgcgacg tgtacggcgc
    17581 cggctatttc tacattcccg gcacggaaac ctgcctgcgc gtcgg
//
LOCUS       DASYCS010000029         3587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2468133,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000029 DASYCS010000000
VERSION     DASYCS010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2468133"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..126
                     /locus_tag="VGM46_01200"
     CDS             <1..126
                     /locus_tag="VGM46_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY4191237.1"
                     /translation="QGKAGFMRAEHGHGVDVMRVIKQALDPLNILNPGKILPSGQ"
     gene            209..>3587
                     /locus_tag="VGM46_01205"
     CDS             209..>3587
                     /locus_tag="VGM46_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HEY4191238.1"
                     /translation="MLARLFVIVGGLLVLALCAALVGPYFVDWTGYRADFEREASAIL
                     GRRVTVQGDATARLLPFPSVTFSNVSVAGGPDGQPAMTVETFSMDAELAPFLRGEALI
                     FDMRLVRPKATIDIADNGAVDWAVRPSSPLHLDQISIEKLTVTDGQISLHHAAGGRSH
                     MLSDINSTVSAKSLAGPWRLDGTLKLDGLSAKLTASTGRAEPTGQMRLRLHIDPDAYP
                     LVIESDGNAGMKNGAAVYSGQFRIAEDEANEVRLRGTDGETVKVKAIKAAKTDKAAKA
                     APAYRLTGKFALDHQKLGIDEFHFETGPLDSPYAADGKASIDLGAKPSFTVEASGAQV
                     QFDETVGGETSTALTLKQRVGALERTLLALPKPTIPGVIEVRLPAVVAGDTIIRDVHL
                     SAEPAPAGWTVKSLEATLPGRTKLEAKGLLGIEKKFGFTGSLLLAVAQPSGFAAWLSK
                     DVDEAIRRLPAAGFKANVEMNDERQTFNDLELILGKAKFSGRIDARTPADARPSMQMK
                     LSGGELDVDGLTAFASLFVSDKGANRFPDRDVDFEVDAGPVTAGGLTADTLDTALRLR
                     DGALEVDRLSIGGLAGASISATGRLQDFPAHPSGKLDASLVAVDLKPLVDAAVRQYPA
                     SPALKGLAARVAAYPELLQDAQIDLVASAAANGDGTTGLALSAEGTAGGTAFSASLSG
                     KGTPQTLQDAPLALTFNAKNSDATTLLALYGLPVLPLGVVGEASTDLSAKGMLTEGLT
                     TAFSLSGQDFKAGFDGAVALGEQGPSAKGKITLDAADAEPWLMTTGMGLPGMGTGMAA
                     NFSADADYANGLLVLDGLSGAINEAAVSGDLNVDVKDGQPHLAGALVLDELDLDPMAV
                     TLFGDEAFVGAGSGKGWPTAPFSEKSATPFSADLDISTAALSAGAFATAYDASLALKL
                     DQSGIRLSDMRGKLYGGALSGSFELKNNQGAGLFSGQMKLGDADLSKMLPDGGLTGKA
                     DLSTELSAGGKSVDGMIAALSGSGTASLKGLTVTGVNPDAFAALIAKADAVGRDIDAA
                     KTAGFAPQIAGEGNFAARDTQFAFTVANGVLRAPPVKLENKAATLTANIAADLGSTTA
                     SAKGSVAYKPGDEVLVGSEPVMNFDIEGPLGAVGRTFD"
BASE COUNT          635 a         1075 c         1255 g          622 t
ORIGIN      
        1 cagggcaagg ccggcttcat gcgcgccgag catggccatg gcgtcgacgt gatgcgcgtg
       61 atcaagcagg cgctcgaccc gctgaacatc cttaatccag gcaagatttt gccgtccggc
      121 caatgatgcc cgcgcgcaac gattgcctct ttatcgaaac ggcgatgcag gtagagtgcg
      181 cacgaccaga aacgattgac tggagctgat gctcgcccgc ctgttcgtaa ttgtcggagg
      241 cctgcttgtg ctggcgctgt gcgcggcgct ggtcgggccg tattttgtcg actggaccgg
      301 ctaccgcgcc gatttcgagc gtgaggcaag cgccattctt ggccgcaggg tcaccgtgca
      361 gggcgatgca acggcgcggc tgctgccgtt cccctcggtg acgttttcca atgtttccgt
      421 cgccggcgga ccggacgggc aaccggcaat gacggtggaa accttttcca tggatgccga
      481 gctggcgccg ttcctgcgcg gcgaagcgct gatcttcgac atgcggctgg tgcggcccaa
      541 ggcaaccatc gacattgccg acaatggcgc ggtggactgg gcggtgcggc cgtcctcgcc
      601 cctccacctg gaccagattt caatcgagaa gctgactgtg acggacgggc agatttcgct
      661 gcatcacgcg gccggcgggc gcagccatat gctgtcggat atcaattcga cggtttcggc
      721 gaagtcgctg gcgggtccat ggcggctgga cggcacgctg aaactcgacg ggctgagcgc
      781 caagctgaca gcatcgacgg gccgcgccga gccgacaggg cagatgcggc tgcggctgca
      841 catcgacccc gacgcctatc cgctggtgat cgagagcgac ggcaacgccg gcatgaagaa
      901 tggcgcggca gtctattccg ggcaattccg catcgccgaa gacgaagcaa acgaggtgcg
      961 gctgcgcggc acggacggcg agacggtcaa ggtgaaggcg attaaggcgg caaagacgga
     1021 taaagccgcg aaagccgcgc cggcatatcg gctcacgggc aagttcgcgc tggatcacca
     1081 gaagcttggc atcgacgagt tccacttcga gacggggccg ctggacagcc cctatgccgc
     1141 cgacggcaag gcttccatcg atcttggcgc caagcccagc ttcaccgtcg aggcgagcgg
     1201 cgcgcaggtg cagttcgacg aaacggtcgg cggcgagaca agtaccgcgc tgacgctgaa
     1261 gcagcgggtc ggggcgctgg agcggacgct gcttgcgctg ccgaagccga ccattccggg
     1321 cgtgatcgag gtgaggcttc cggcagtcgt tgccggcgac accatcattc gcgacgtgca
     1381 tctttcggcg gaaccggccc ccgccggctg gacggtcaag tcgctggagg cgacgctgcc
     1441 cggacgcacg aagctggagg ccaagggcct gctcggcatc gagaagaaat tcggcttcac
     1501 tggttcgctg ctgctggcgg tggcgcagcc atcgggtttc gcggcctggc tgtcgaagga
     1561 tgtggacgag gcgatccggc ggcttcccgc tgccgggttc aaggccaatg tcgagatgaa
     1621 tgacgagcgc cagacgttca acgatctgga attgatcctc ggcaaggcga agttttcagg
     1681 gcgcatcgat gcaagaacgc ctgccgatgc caggccatcg atgcagatga agcttagtgg
     1741 cggcgaactc gatgtcgatg ggctgaccgc gttcgcctcg ctgttcgtca gcgacaaggg
     1801 cgcgaaccgc tttcccgacc gtgacgtcga tttcgaggtc gatgccggcc cggtgacggc
     1861 aggcgggctg accgccgata cgctcgatac ggcgcttcgg ctgcgcgacg gagcgctgga
     1921 ggtggaccgg ctttccatcg gtggattggc cggcgcgtcg atcagcgcaa ccggccgatt
     1981 gcaggatttc ccggcccatc cgagcggcaa gctcgacgct tcgctggtcg cggtggactt
     2041 gaagccgctg gtcgatgccg ccgtccggca atatcccgcc agcccggccc tgaagggtct
     2101 cgcggcgcgg gtggctgctt accccgaact gttgcaggac gcgcagatcg atctggtggc
     2161 cagtgcggcc gccaatggcg acggcaccac cggattggcg ctgagcgcgg aaggcactgc
     2221 cggcggcacg gctttttcgg cctctctgtc gggaaaggga acgccgcaga cccttcagga
     2281 tgcgccgctg gcgctgacgt tcaatgcgaa gaacagcgac gccacgacgc tgctggcgct
     2341 ttacggactg ccggtgctgc cgcttggcgt ggttggtgag gcgagcaccg atctgtccgc
     2401 caagggcatg ctgaccgagg ggctgacgac ggcgttcagc ctttccgggc aggacttcaa
     2461 ggccgggttc gacggcgcag tagcgcttgg cgagcaaggg ccgagcgcca agggcaagat
     2521 cacgctggac gcggccgatg ctgagccgtg gctgatgacg acgggcatgg gcctgcccgg
     2581 catgggcacg ggcatggcgg cgaactttag cgccgatgca gattatgcaa acggcctgct
     2641 ggtgctggac ggactttccg gcgcgatcaa cgaggcggcc gtttcgggcg atctcaatgt
     2701 cgatgtgaag gacgggcagc cgcatctggc cggcgcgttg gtgctggacg aactcgacct
     2761 cgacccaatg gcggtgacgc tgttcggtga cgaagccttc gtcggggcag ggagcggcaa
     2821 gggctggccg acagccccgt tcagcgaaaa gtcggctacg ccgttcagcg ccgatctcga
     2881 tatcagcacg gccgcgcttt cggcgggcgc gttcgccacg gcctatgacg cttcattggc
     2941 gttgaagctc gaccagagcg gcatccgcct ttcggacatg aggggaaagc tttatggcgg
     3001 cgcgctttcc ggctcattcg agttgaagaa caatcagggc gccgggctgt tttccggcca
     3061 gatgaagctt ggcgatgcag acctgtcgaa aatgttgccg gatggcggct tgaccggcaa
     3121 ggccgatctg tcgaccgaac tttccgccgg cggcaaatcg gtcgatggga tgatcgccgc
     3181 gctttccgga tcgggcacgg cctcgctgaa aggcttgacg gttacgggcg tcaatccgga
     3241 cgcgtttgcg gctcttatcg ccaaggcgga tgcggttggg cgggatatcg acgcggccaa
     3301 gacggccggg tttgcgccgc agattgcggg cgaaggcaat ttcgcggcgc gcgatacgca
     3361 atttgccttt acggtcgcca atggcgtgtt gcgcgcgccg ccggtcaaac tggaaaacaa
     3421 ggcggcgacg ctgacggcca acattgccgc cgaccttggc tcaaccacag catcggccaa
     3481 gggcagcgtg gcctacaagc ccggcgacga ggtattggtc ggctcggaac cggtgatgaa
     3541 tttcgatatc gaagggccgc ttggcgcggt tggccgcacg tttgaca
//
LOCUS       DASYCS010000030        22228 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4209044,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000030 DASYCS010000000
VERSION     DASYCS010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 22228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22228
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4209044"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1076)
                     /locus_tag="VGM46_01210"
     CDS             complement(<1..1076)
                     /locus_tag="VGM46_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006334822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HEY4191239.1"
                     /translation="MAVEAGSAGDLVQVVALLAAGVVAVPIFKRAGLGSILGYLAAGV
                     VIGPFGLGIFTESATILHVAELGVVMFLFIIGLEMQPSRLWGLRREIFGLGALQVGVC
                     ALLLTAIGWLGGFPVAQSFLAGTGFVLTSTAIVMQVLEERGDIAAPKGQRVISILLLE
                     DLAIVPLLALVAFLAPGGAETSLSERLAEIGIGLAAIAGLVIAGRYLLNPFFRILADS
                     RAREVMTAAALLVVLGSALVMQLSGLSMAMGAFLAGVLLSESTFRHQLEVDIEPFRGI
                     LLGLFFLAVGMSLDLRVVADNWQLIAIYVVAYMVMKAVGIYGVARFLKTGHREALERA
                     VIMAQGGEFAFVLYAAAAAVGLID"
     gene            complement(1252..1779)
                     /locus_tag="VGM46_01215"
     CDS             complement(1252..1779)
                     /locus_tag="VGM46_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018427776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191240.1"
                     /translation="MPFALALSMTVGATANAFSADQDKQFFQSIEGHWAGPGEIVAGK
                     YKGTKFTCNFTGSTPEGKIGMTLDGGCRVGVFTQKMSAKIENGGRDGYKGSFMGGSEG
                     AGLDIISGNVVDPHKVVFGINRKQLRGVMQARLPDDGSMIVTISVKVEKQMIPVIGMS
                     MKRLDVTEVGSIAPN"
     gene            complement(1935..3020)
                     /locus_tag="VGM46_01220"
     CDS             complement(1935..3020)
                     /locus_tag="VGM46_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HEY4191241.1"
                     /translation="MMAPRKPTAFRMEPEIDPAERPTSAGRDGGSSHPARKPQAMKTE
                     AVTVAPAEFDVFDEPDIVSAEPPPASAPKKRSLLAHIFLGAFSVLVSLGIGLWVDGLI
                     RDLFQHAPWLGWLAFAVAIIAVAALAVILAREFLAISRLAEVEKLQGRALDAINRNDG
                     KEARRVVGELTKFVAGKPETAAGRRALAELKDDIIDGRDLMRLAETEILGPLDARAKI
                     MILDAAKRVSLVTAVSPRALVDVAYVVFEAGRLIRRLSELYGGRPGTLGFFRLARSVL
                     AHLAVTGSIAIGDGFVQQIVGHGLAARLSAKLGEGVVNGMMTARIGIAAMETARPLPF
                     TAVRRPGMGDFLSALTSFATARSKPSS"
     gene            complement(3017..4483)
                     /locus_tag="VGM46_01225"
     CDS             complement(3017..4483)
                     /locus_tag="VGM46_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HEY4191242.1"
                     /translation="MASSMTSFADEARIALDTLAGRATGLFSPSLRLGVTGLSRAGKT
                     VFISSLVHNLVHGGRLPLFEARKSGRIARAFLEQQPDDAVPRFQYEDHIAALVDDRVW
                     PDSTRAISEMRLTIEYESASGWNRMFSAGKLSVDIVDYPGEWLLDLPLLGKSFAEFSR
                     EAFEMAALPVRADLSEHWRNVSAPVDPGSDADEMTARRLAESFAAYLKACKQDERALS
                     TLPPGRFLMPGDLEGSPALTFAPLPNLDAGRARTGSLHAMMERRYEAYKTHVVKPFFR
                     EHIARLDRQIVLVDALQALNAGPAAVADLERAVTEILACFRPGRGNFLTDIYSRRIDR
                     ILIAATKADHLHHESHDRLQAIVRRLADRAIARANFTGAKVDVVAMAAVRATREGTVK
                     QGQDVLPVIIGTPLEGERIDGEIFDGKTETAIFPGDLPEKPGAAFIAAEQGSTDPLIR
                     FVRFRPPKLERTAEGVTLSLPHIRLDRALQFLIGDRLA"
     gene            complement(4551..5054)
                     /locus_tag="VGM46_01230"
     CDS             complement(4551..5054)
                     /locus_tag="VGM46_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY4191243.1"
                     /translation="MSRLYLVRHAKAGWAMPGVRDFDRPLDASGIADAETLGAAMRAG
                     RHVPDLTICSNALRARETLAGLAANADTGRVVFTDVLYSGDANSYLATIRENGARGSL
                     LLIGHNPMMEDLALAVSGDGDETARATLAYGFPTCGLAAIRFEGSLGEARLGSGYLEV
                     FLTPAEM"
     gene            5318..5734
                     /gene="dksA"
                     /locus_tag="VGM46_01235"
     CDS             5318..5734
                     /gene="dksA"
                     /locus_tag="VGM46_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010660068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEY4191244.1"
                     /translation="MNEIAATDYVPSEDEPFMNERQKSYFRQKLLTWKNDIFREARET
                     LEILQQENANHPDLADRASSETDRAIELRARDRQRKLISKIDSALQRLEEGTYGYCEE
                     TGEPIALKRLDARPIATLSIEAQERHERRERVYRDD"
     gene            complement(5850..6569)
                     /locus_tag="VGM46_01240"
     CDS             complement(5850..6569)
                     /locus_tag="VGM46_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191245.1"
                     /translation="MLDWLDGIAGPDYARAILWTLAALILLVVVLVIVKLIRSMTFGT
                     FVAGGRNRKTRLAVMDATAVDSHRRLVLVRRDDIEHLLLIGGPTDVVVERDIRLVGSR
                     RPAPAENGGQAIPAPRMQPVPASARQAEPKPATREPQPAHQRDQAVRPSSVSTSTTAA
                     GISPPTYPAVKTPAPHAAPRMEISEPDDIDAAFMSELDVSLEDFSSDKPTPQPSSSKR
                     ASASLDDEMTKLLGELSTHKK"
     gene            6773..9358
                     /locus_tag="VGM46_01245"
     CDS             6773..9358
                     /locus_tag="VGM46_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006334802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY4191246.1"
                     /translation="MAKETRGDFYPVPMVNQSTKPGAVTRLVVFIVVLTAAAIIFGIF
                     RDRLGDPFLLGMLGVLAMIGVGFLFATAIGFVHVAPRSTSDELSKAFIDSMGHGLLVT
                     DAKGRVIYANRAYADMTGAASAADLKTVEALLADVPEASATINRLSSGLSDGLVGSGE
                     FRLVQSIRPGAEAGARWYRTRARTFNVPGQRQPMLAWELADISQERAEQERFFLDLQQ
                     AIDHLDHAPAGFFSADQEGRVTYINATLAEWLGIDLTSFTPGSVALPEIVAGDGMALV
                     RSVKADPGTTRNAVIDLDLTTMTGEVLPVRFMHRVSASREGIGGPTRTIVLNRTQGED
                     ASADLRASEVRFTRFFNSTPMAIAGVDQGGRILRTNAPFLSLFSSVVDRDALDRRVRL
                     DTVIHERDRAAFATAFERARQRQADIAPIDTVLPGNDERHIRFYVNAVADSAAGEGAE
                     EAAIVYAVETTEQKALEGQMAQSQKMQAVGQLAGGIAHDFNNVLTAIIMASDLLLTNH
                     RPSDPSFPDIMNIKQNANRAASLVRQLLAFSRKQTLRPEVLNLTDVMADLRMLLARLV
                     GNDIKLKVEHGRDLWPVKVDLGQFEQVIVNLTVNARDAMPTGGELMVRTRNVTAEESR
                     NFSYRELTSADYVLVETEDTGSGITPEVLKKIFEPFFTTKDVGKGTGLGLSMVYGIIK
                     QTGGFIFCDSEVGKGTVFRIFLPRYVAEVRTSSETGLDVPVAAAPVPKIADAAKDLSG
                     SATVLLVEDEDAVRMGGVRALTSRGYTVHEASSGVEALEVFAELEGKVDIIVSDVVMP
                     EMDGPTLLVEIRKLQPDIKFVFVSGYAEDAFARNLPADAQFGFLPKPFSLKQLATVVK
                     DVLES"
     gene            complement(9353..9763)
                     /locus_tag="VGM46_01250"
     CDS             complement(9353..9763)
                     /locus_tag="VGM46_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191247.1"
                     /translation="MPRRLVFLALPLLASLSGPATASAGGGVLPDGAYRCEVYMLGMF
                     IYLGEIVIKGDVYSGPSIVGGMQQGYNYQISPRGEISWLGPLGGYTAGGNSVSLTQVT
                     ADGQHDASFDIILRQPNGSFTASTCTIGGTAGKL"
     gene            9975..10151
                     /locus_tag="VGM46_01255"
     CDS             9975..10151
                     /locus_tag="VGM46_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191248.1"
                     /translation="MGHFVEDYQKRRLTERVDILSAVNILKSKGYDNDDLITEIIRVF
                     YVDLDTYNDIVKAA"
     gene            complement(10205..10444)
                     /locus_tag="VGM46_01260"
     CDS             complement(10205..10444)
                     /locus_tag="VGM46_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191249.1"
                     /translation="MVDLGRPERLQIMLSADELLALENWRFDKRMPSRAAAVRELLRR
                     GLAGDGFLTADNGSKSQDFGVIDGHSQQLSDDSAG"
     gene            10733..10969
                     /locus_tag="VGM46_01265"
     CDS             10733..10969
                     /locus_tag="VGM46_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191250.1"
                     /translation="MDVQLSPQILDDMTHSLQAALAKNGIINILQLAEEIRRRNEVEN
                     IALEDVAAKLMLKAQLFNAAMEFDSEADISPLAK"
     gene            complement(11288..12820)
                     /locus_tag="VGM46_01270"
     CDS             complement(11288..12820)
                     /locus_tag="VGM46_01270"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007532797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEY4191251.1"
                     /translation="MKDVLKELERRREVARAGGGQARILAQHKKGKLTARERIEIFLD
                     EDSFEEFDMFVEHRSTDFGMDSQKIAGDGVVTGWGTINGRPVYLFAKDFTVFGGSLSE
                     AHAEKVQKVQDMALRNRAPIIGIYDAGGARIQEGVAALGGYAEIFQRNVLASGVIPQI
                     SVIMGPCAGGDVYSPAMTDFIFMVRDTSYMFVTGPDVVKTVTNETVTAESLGGASVHT
                     TKSSIADGAYDNDVEALLQMRRLVDLLPASNTAEIPEVECYQSVTDHDLSLDRLIPDN
                     ANKPYDIKELILKTCDEGDFFEISQAFAKNIVTGFGRIEGRTVGFVANQPMVLAGVLD
                     SDASRKAARFVRFCDCFNIPLVTFVDVPGFLPGTAQEYGGLIKHGAKLLFAYGEATVP
                     KITVITRKAYGGAYDVMASKHLRGDMNYAWPTAQIAVMGAKGAVEIIYRKDIGDAEKI
                     AAHTKGYEDRFLSPFVAAERGYIDEVIMPHSTRRRIARALRMLRNKDLQNPWKKHDNI
                     PL"
     gene            13229..14602
                     /locus_tag="VGM46_01275"
     CDS             13229..14602
                     /locus_tag="VGM46_01275"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015317958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY4191252.1"
                     /translation="MASADHDTDQLTGFAALGISGVLLKATHAAGFTEPKPIQVQAIP
                     PQLEGRDIFGIAQTGSGKTAAFSLPILTKIMALGTKRRAKTCRALILAPTRELAVQIE
                     DTIKILAKGAHVSTALVLGGVSRFSQVKKIAPGVDILIATPGRLTDLVREGDLVLADT
                     KWLVLDEGDRMLDMGFINDVKRIARATAPDRQTALFSATMPDEIAELAKGLLKNPIRI
                     EVSPQSTTVAEISQGVIFARTKQKRQVLSTMLADEAMKSVIIFSRTKHGADRVTKDLE
                     RDGFKAAVIHGNKSQNARQKALSDFREGSVRILVATDIAARGIDVPGISHVVNFDLPD
                     EAESYVHRIGRTGRNGRDGIAVTLCDPSENAKLRQVERIIRAKLPIVADHLASPDPVR
                     NPAEKNEREYVDPRRVKRPGGANQNNSKRFGDKPTGERKADGAKPPFKGNNKRRFGGK
                     RPAARAA"
     gene            14839..15873
                     /locus_tag="VGM46_01280"
     CDS             14839..15873
                     /locus_tag="VGM46_01280"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006333450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEY4191253.1"
                     /translation="MVGSVIDAIGNTPLIRLKRASQETGCEILGKAEFMNPGQSVKDR
                     AGLFIIRDAEKRGLLKPGGVIVEGTAGNTGIGLTLVAKALGYRTVIVIPETQSQEKKD
                     TIKLLGAELIEVPAVAYKNPNNYVKLSGRLAEQMAGTEPNGAIWANQFDNVANRDGHI
                     HTTAQEIWQQTGGKVDGFVSAVGSGGTLAGVAAGLRDKNRDVKIALADPLGAALYNFY
                     TAGELKSEGSSITEGIGQGRITANLDGFAPDFSFQVPDDEALPIIFDLIQEEGLCVGG
                     STGINIAGAIRLARELGPGHTIVTILCDFGTRYQSKLFNPAFLRTKNLPVPGWMEHKS
                     EISVPFEAAA"
     gene            15870..16616
                     /locus_tag="VGM46_01285"
     CDS             15870..16616
                     /locus_tag="VGM46_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008875709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HEY4191254.1"
                     /translation="MSVRKTEILFRDDSYLKTATARVEAINERGGIILDRTIFYATSG
                     GQPGDTGFLERADGSRIAISATLTGETKDEIIHVPSPDQPIPAVGEELKLVIDWPRRH
                     LLMRMHAACHLLTVVCPFPITGAAVSQDDSRVDFDMPDFSMTKEEVTARLMELVRADH
                     PIFIRSITEEELAANPTLVKSKNVRPPTGTGHIRLVCIGENASIDSQPCGGTHVKSTG
                     EVGEIHIGKIEKKGRENRRFRIRFGALPAA"
     gene            16663..17514
                     /gene="sseA"
                     /locus_tag="VGM46_01290"
     CDS             16663..17514
                     /gene="sseA"
                     /locus_tag="VGM46_01290"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006205420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HEY4191255.1"
                     /translation="MAQDSPFTVDADWLQEKLGQPGFAIVDASWYLPAQNRDARAEYE
                     AAHIPGAVFFDQDAVSDRTSPLPHTLPSPEDFARYVGAMGISADDTIIVYDGPGFFSA
                     PRAWWMFRVMGVFQVYVLEGGLDRWKAAGRPVTAQTTKVAPCIFHADFDSSRVASLAD
                     MRHIVDGHESQIADARGPGRFAGTEPEPRAGIRSGHMPGARNVPASMLSQDGQLLGKD
                     RLRKIIVDAGIDLSRPVVTSCGSGITAAALTLALETIGHTDNRLYDGSWTEWGGLADT
                     PVATGKE"
     gene            17516..18082
                     /locus_tag="VGM46_01295"
     CDS             17516..18082
                     /locus_tag="VGM46_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018427791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY4191256.1"
                     /translation="MVNSSQPDDIEVTVTFLEMETPPAYYPALPYNRQIALLKAKDIP
                     LHFYRYLTDRVGRKWHWVNVLRLDDDELAASLHREDRDIRILYLDGAPAGYFDVKPHL
                     PDETELAYFGMMDHAMGQGLGRWFLGAAISACWAHGPKRVTVQTCTLDHPAALPLYQK
                     LGFTPVGQKKELVRPMPFAERSASVMRP"
     gene            18184..18741
                     /locus_tag="VGM46_01300"
     CDS             18184..18741
                     /locus_tag="VGM46_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006333467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY4191257.1"
                     /translation="MITRRVFAAAMIAALAVPGMAFAQARTPSGEQIERRLEISPRMR
                     LRPNQRVTIQEFKRRPDLRRAAPSIDIQSINFAFGSAEIPNSQYDKVENIADGLRRIQ
                     RRQPRARVLIEGHTDAVGSFAANQALSQDRAASLKRTLVREFGVPARMLETVGYGEEF
                     LLVPTQSENWQNRRVTLRRFDDFIR"
     gene            complement(18981..20096)
                     /gene="ald"
                     /locus_tag="VGM46_01305"
     CDS             complement(18981..20096)
                     /gene="ald"
                     /locus_tag="VGM46_01305"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEY4191258.1"
                     /translation="MRVGCLKEIKNHEYRVGLTPGSVREYVAHGHEVLVERGLGEGIG
                     ADDNAYRAAGATIAKTAAEVFAQCDMIVKVKEPQPGEWAQLREGQILYTYLHLAPDPE
                     QTKGLLASGVTAVAYETVTDDRGGLPLLAPMSEVAGRLSIQAGATALQKANGGRGVLL
                     GGVPGVLPGKVTVLGGGVVGLHAARMAVGLGADVSIIDRSIPRLRQLDDIFNGRVHTR
                     YSTVEALEEECFSADVVIGAVLIPGATAPKLVTREMLSGMKKGAVLVDVAIDQGGCFE
                     TSHATTHADPTYVVDGIIHYCVANMPGAVPVTSAHALNNATLHYGLQLADKGLKALID
                     DSHLRNGLNVHKGKITNRAVADALGYELSEPKAVLAA"
     gene            20251..20724
                     /locus_tag="VGM46_01310"
     CDS             20251..20724
                     /locus_tag="VGM46_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006205413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY4191259.1"
                     /translation="MPLDRIDIAILDALQKEGRLPNAALAEKVGLSQSACSRRLDNLE
                     KSGAIRGYHARLSNAALGHQMTAIVHISLSGQFEKTLSDFEAAIKRCPNVLSCHLMSG
                     EYDYVLRIASRDLADYERIHKEWLSAMPHVTKINSSFALREIVDRTAMGLKSDMA"
     gene            20780..20908
                     /locus_tag="VGM46_01315"
     CDS             20780..20908
                     /locus_tag="VGM46_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191260.1"
                     /translation="MRYVIIIAAIAFFIIWDGLFNEGRYLDHSVREVKTVMRDITG"
     gene            21054..21344
                     /locus_tag="VGM46_01320"
     CDS             21054..21344
                     /locus_tag="VGM46_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008875728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HEY4191261.1"
                     /translation="MIRHIVFFSAKHKDEVEAIRFGLMALGKIPHSQLFEVSLNSKVD
                     PFGDEIDVVVYAEFEDHAALAAYKAHPLYAETTAKVRPKRELRFSADVPAGG"
     gene            complement(21563..21637)
                     /locus_tag="VGM46_01325"
     tRNA            complement(21563..21637)
                     /locus_tag="VGM46_01325"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21603..21605),aa:Asn,seq:gtt)
     gene            21840..22103
                     /locus_tag="VGM46_01330"
     CDS             21840..22103
                     /locus_tag="VGM46_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015317514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191262.1"
                     /translation="MTAGNDGHAAPARKISVFGREFKMPRSRWARIAIGLALTILGLF
                     GFLPILGFWMVPLGLLVLSYEFASVRRLRRRFVIWWGRRRRRS"
BASE COUNT         4438 a         6732 c         6755 g         4303 t
ORIGIN      
        1 ttgtcgatca ggccgaccgc ggccgctgcg gcataaagca caaaggcgaa ttcgccgccc
       61 tgcgccatga tgacggcgcg ctccagcgcc tcgcggtgac cggttttcag gaagcgggcg
      121 acgccgtaaa tgccgacggc cttcatcacc atataggcga cgacatagat ggcgatcagc
      181 tgccaattat cggcaacgac cctaagatcg agcgacatgc ccaccgccag gaagaacagg
      241 ccaagcagga tgccgcgaaa gggctcgata tcgacttcga gctggtggcg aaacgtcgat
      301 tccgacagta atacaccagc gaggaatgcg cccatcgcca tcgacaggcc gctaagctgc
      361 atgacgagcg ccgaccccag caccacgaga agcgctgccg ccgtcatcac ctcgcgggcg
      421 cgggaatcgg ccagaatgcg gaaaaacggg ttgagcagat aacgcccggc gataaccagc
      481 ccggcgattg ccgcaagtcc gatgccgatt tctgcaagcc gttcggacag gctggtttcc
      541 gcgccacccg gcgcaagaaa ggcaaccaat gccagcagcg gcacgatcgc caaatcttcg
      601 aggagaagaa tggagattac ccgttgcccc ttgggggcgg cgatgtcgcc gcgctcctcc
      661 aggacttgca tgacgatagc cgtggaagtg agcacgaagc cggtgccggc caggaaggat
      721 tgcgcaaccg ggaagccacc gagccagccg atggctgtca gcaggagcgc gcaaacgccg
      781 acctgcaatg cgccgagccc aaagatttcg cgccgcagtc cccataggcg agacggctgc
      841 atttcgaggc cgatgatgaa caggaacatc accacgccaa gttcggcgac atgcaggatg
      901 gtcgcggatt cggtaaagat gccgaggccg aacggtccga tgacgacgcc ggctgccaga
      961 tatccgagga tagaaccgag gccggcccgc ttgaagatcg gaaccgcgac cacgccggcg
     1021 gcaagcagcg ccaccacctg aaccagatca ccggccgagc ctgcttctac cgccattcct
     1081 gccttttccg atccatggag cgcggatatc caccgctcgt cttggccaag ataggagttc
     1141 ccatggcgcg agaacagcct gaaaaaggcc ggcgggcgtt cactttgcgg ccaaatggca
     1201 gatcatgggc gcaaacgacg cgatggaacg cggcagcggt ggaaagacga attagttcgg
     1261 tgcgatagag cctacttcgg tgacgtccag ccgcttcatg ctcatgccga tgaccgggat
     1321 catctgcttt tcgaccttca cggatatggt gacgatcatc gagccgtcat cgggaaggcg
     1381 tgcctgcatg acgcctcgca actgcttgcg gtttatgccg aaaacgacct tgtgcggatc
     1441 gacgacattg ccggaaataa tgtcgaggcc agcgccttcg gagccgccca tgaagctgcc
     1501 cttgtagccg tcccgaccgc cattctcgat ttttgccgac attttctgtg tgaagacgcc
     1561 gacgcggcag ccgccgtcaa gcgtcatgcc gatctttccc tcgggagtgg aaccggtgaa
     1621 gttgcaggtg aatttcgtac ccttgtattt gccggcgaca atttcgcccg gcccggccca
     1681 gtgaccttcg atggactgga agaactgctt gtcctgatcg gcgctgaagg cgttcgccgt
     1741 tgccccgaca gtcatcgaca gagcaagcgc aaagggcatt acgcaagggt agattgcgct
     1801 tttcatcgat agcacccgga acaagttttc atgcggtgga catttgcccg aaccatgaac
     1861 aagattggtt aatgcttcgt cacccggcgc gtgcgcaagg gtgttttgag ctgggtgctg
     1921 cggttaaatc cggctcagga agacggtttc gaccgcgctg tcgcaaacga cgtcagggca
     1981 gagagaaaat cacccatgcc gggccgcctg acggcggtga agggcagggg ccttgccgtt
     2041 tccatggccg cgattccgat tcgtgccgtc atcatgccgt tcacgacacc ttcgccaagc
     2101 ttggcggaaa gccgcgccgc aaggccgtgg ccgacgatct gctgaacgaa accatcgccg
     2161 atggcgatgg agccggtaac ggccagatgg gcaagcacgc tgcgcgccag ccggaaaaac
     2221 cccaaagttc ccggtcgtcc gccgtaaagc tccgacagcc gccggatcag gcggcccgct
     2281 tcaaacacga cataagcgac atcgaccagt gcgcgcgggc tgaccgccgt aaccagcgac
     2341 actcgcttgg ccgcatccag gatcatgatc ttggcgcggg cgtcgagcgg cccgaggatt
     2401 tcggtttcgg caagccgcat gagatcgcgg ccatcgatga tatcgtcctt cagttcggcc
     2461 agcgcccggc ggcctgccgc cgtttcaggc ttgccggcga cgaatttcgt cagttcgcca
     2521 accacgcgcc tggcttcctt gccatcattg cgattgatgg cgtccaacgc ccgaccttgc
     2581 agtttttcca cttcggccag gcgggaaatc gccagaaatt cgcgcgcaag gatgacggcg
     2641 agcgccgcta cagcgatgat cgccaccgca aaggccagcc agccgagcca cggcgcatgc
     2701 tggaagaggt cgcggataag tccgtctacc cacaggccta tgccaaggga gaccagaacg
     2761 ctgaaagcgc cgaggaagat atgcgcgaga agggagcgct ttttcggcgc gctggctggc
     2821 ggcggctcgg ctgaaacgat gtcgggttca tcgaaaacgt cgaattcggc cggcgcgaca
     2881 gtgaccgctt cggtcttcat cgcctgcggc ttcctggccg gatgcgatga cccgccatcg
     2941 cggcctgcgg aagttgggcg ctctgccgga tcgatttcag gctccatgcg gaaggccgtc
     3001 ggttttcgtg gcgccatcat gcgaggcgat ctccgatgag gaactgcaag gcgcggtcca
     3061 gccggatgtg cggcagggac aaggtgacgc cttcggcggt tcgttcaagt tttggcgggc
     3121 gaaagcgcac gaagcggatc agcggatcgg tgctgccttg ctcggcggct ataaaggctg
     3181 ctccgggctt ttccggcagg tcgcccggaa atatggctgt ttccgtcttt ccgtcgaaga
     3241 tttcgccatc gatccgctct ccttcaagcg gcgtgccgat gatgactgga agtacgtcct
     3301 gcccttgctt gaccgtgcct tcgcgtgtgg cgcgcaccgc cgccatggcg acgacgtcga
     3361 cctttgcgcc ggtgaaattt gcgcgggcaa tggcacggtc ggcgaggcgc cgcacgattg
     3421 cctgcaagcg gtcatggctt tcatgatgca gatggtcggc cttggtcgcg gcgatgagaa
     3481 tgcggtcgat ccggcgcgaa tagatgtcgg tgaggaaatt gccgcggccg gggcgaaaac
     3541 aggcaaggat ttcggtcacg gcgcgctcaa gatcggccac ggcggcggga ccggcgttga
     3601 gcgcctgtag cgcatcgaca agcacgatct gccggtcgag gcgcgcaata tgttcgcgga
     3661 agaacggctt gaccacatgc gtcttgtagg cctcgtagcg gcgttccatc atcgcatgca
     3721 gcgaacctgt tcgcgcgcgt cccgcatcaa ggttgggcag gggcgcgaaa gtcagcgcgg
     3781 gcgatccctc aaggtcgccc ggcatcaaaa accggcccgg cggcagcgtg gaaagcgccc
     3841 gttcgtcctg cttgcaggcc ttcaggtagg cggcaaagct ttcggccagc ctgcgcgccg
     3901 tcatctcgtc ggcatccgaa ccgggatcga cgggagcgct aacgttgcgc cagtgttccg
     3961 acaggtcggc ccgcacaggc agcgccgcca tttcgaatgc ttcccgcgaa aattcggcga
     4021 aggatttgcc gagcagcggc aggtcgagca gccattcacc tggataatcg acaatatcca
     4081 cggaaagctt gccggcggaa aacatgcggt tccagccgga cgccgattca tattcgatgg
     4141 tcaggcgcat ttctgaaatg gcgcgcgtcg aatccggcca gacacggtca tcgaccagcg
     4201 ccgcgatatg atcctcatac tgaaaacgcg gaacggcgtc atccggctgc tgttcgagga
     4261 aggcgcgcgc gatgcggccc gatttccggg cttcaaacag cggcagccgc ccgccatgca
     4321 ccagattatg cacgagggag gaaatgaaca ccgttttgcc ggcgcgcgac aggccggtaa
     4381 cgccgagccg gagcgaagga gaaaaaagcc ccgtcgcccg cccggccagc gtatcgagcg
     4441 cgatccgggc ttcgtcggca aaggaagtca tcgatgatgc caatggagac tccctcggtc
     4501 tttctaccag aatataggca gtagcgggcg ctttggaata tccggctgaa ctacatctcc
     4561 gccggtgtca ggaaaacttc caggtaaccg cttccaagcc tggcttcgcc gaggctgcct
     4621 tcaaagcgta tcgctgcaag gccgcaggtg ggaaagccat aagccagcgt agccctggcg
     4681 gtctcgtcgc cgtcgcctga aacagccaag gcaaggtctt ccatcatcgg gttgtgaccg
     4741 atgagcagca gcgagccacg ggcgccattt tcacggatgg tagccagata gctgttggcg
     4801 tcgccgctat aaagcacgtc ggtgaagacg acgcggccgg tatcagcgtt ggcggcaagg
     4861 cccgccaggg tttcgcgcgc acgcagcgca ttggaacaga tggtcaggtc gggaacatgg
     4921 cggccggcgc gcatcgccgc gcccagcgtt tcggcatcgg ctatgccgga cgcatcgagc
     4981 ggacggtcga aatcgcgcac tccgggcatt gcccatccgg ctttcgcgtg cctgacaaga
     5041 tagagcctgc tcacgcaccc ctcccattgc acagggcgcg cattttaccc cggtgaaaca
     5101 ggattagccg tcgcaagcgc tttaagcaac gtcgccgcaa ttgcggctaa gcgcaacgat
     5161 acaaacgaaa ttaaggccgt atgattgacg gccggcatgg atgcgttcgt cggtacaaat
     5221 cgattcaata atgcctgttc ccgataccgt ttgctcttgt gcgcgctccg actgacgaat
     5281 atataggccc caaattgtgg gttttcgggt gagtcgaatg aacgaaatcg cagcaaccga
     5341 ttacgtgcct tccgaagacg agcccttcat gaacgaacgg caaaagtctt atttccgcca
     5401 gaagctgctt acctggaaga acgatatttt ccgcgaggcg cgtgaaaccc tcgaaatctt
     5461 gcagcaggaa aacgcgaacc accccgacct tgccgatcgc gcatcgtccg aaaccgaccg
     5521 cgccatcgag cttcgtgccc gcgatcgcca gcgcaagctg atttccaaga tcgattcggc
     5581 gcttcagcgg ctggaagagg gcacgtatgg ctattgcgaa gagacgggcg agccgattgc
     5641 gctgaagcgt ctcgatgcgc gcccgatcgc aactctgtcg atagaggcgc aggaacgtca
     5701 tgagcgccgc gaaagagttt atcgcgacga ttgatgcgcc tgaatatcca agcattaatt
     5761 cgccagacac cgatccgaat ggccgctgat gcggccattt tcatgtgcct cgtacggttg
     5821 gttgagaccg cgcccttaag gcatgagcct tattttttgt gggttgaaag ttcgcccagg
     5881 agctttgtca tctcgtcgtc gagagaagcg gaagcccgct tcgaggacga tggctgcggc
     5941 gttggtttgt cggacgaaaa atcctccagc gatacgtcaa gctcgctcat gaacgctgcg
     6001 tcgatgtcat cgggttcaga gatttccatc ctgggagccg catggggagc cggcgttttt
     6061 actgccggat aggttggcgg cgaaattccg gccgccgtcg tggatgttga aacagaggaa
     6121 gggcgtacgg cctggtcacg ctggtgggcc ggctggggtt cccgcgttgc cggctttggc
     6181 tccgcctgtc gagcggatgc cggcaccggt tgcattcttg gcgctggaat agcctggccg
     6241 ccattttccg caggtgctgg ccgccgcgaa ccgaccaatc ggatatcgcg ctcgaccacc
     6301 acatccgtcg gccccccgat cagcaacagg tgttcgatgt cgtcacggcg aacaagaacc
     6361 agccggcgat ggctgtcgac ggcggttgcg tccatgacgg caaggcgcgt cttgcggttt
     6421 ctgccgccgg cgacaaaggt tccgaaagtc atgctgcgga taagcttgac gatgacgagc
     6481 acgaccacca gaaggatcag cgcggcgagg gtccacagaa tcgcccgcgc atagtccggc
     6541 ccggcaatgc catccagcca atccagcatc gtcacccccg catctcgtat ggcgtgacac
     6601 tagcttgttg cggctggcta ccgcaagttg ctttcacatt acgtgattga tcgcgcacaa
     6661 ctcttacgtt tttcacgatc atttgcagcc aaggcctttt cggggcatgg agaatgtgat
     6721 tcaagcgaca ggggcgattt tcaggggcag aggaatcgtg agcagggggc acatggccaa
     6781 ggaaacgcgc ggcgatttct atccggttcc gatggtcaat cagagcacca agccgggcgc
     6841 cgtcacgcgc cttgtcgtgt tcatcgtcgt tctcaccgcc gccgccatca tattcggcat
     6901 ctttcgcgat cgtctgggcg acccgttcct gctcggcatg ctgggcgttt tggcgatgat
     6961 cggcgttggt ttcctgtttg ccaccgcgat cgggttcgtc catgtcgccc cgcgctcgac
     7021 cagcgatgaa ttgtcgaagg cctttatcga ttcgatgggg catgggctgc tggtcaccga
     7081 cgccaagggc cgcgtcatct acgccaaccg cgcctacgcc gatatgacgg gcgctgcatc
     7141 ggccgccgat ctcaagactg tggaggcctt gctggccgat gtgccggaag cttcggccac
     7201 catcaatcgc ctgtcatcgg ggttgagcga cgggcttgtc ggcagcggcg agtttcgttt
     7261 ggtgcaatcg atccgtcccg gcgccgaagc cggcgcgcgc tggtatcgaa cccgcgcccg
     7321 caccttcaat gtgcccggac agcgccagcc catgctggca tgggaacttg ccgacatttc
     7381 gcaggagcgg gccgaacagg agcgcttttt cctcgatctg caacaggcga tcgaccatct
     7441 cgaccatgcg cctgccggtt tcttctccgc cgatcaggaa gggcgcgtga cttatatcaa
     7501 tgcaacgctt gccgaatggc tgggcatcga cctcaccagc ttcacgccag gctctgtcgc
     7561 cctgccggaa atcgttgcag gcgatggcat ggcgcttgtc cgctcggtca aggccgaccc
     7621 cggcacgacg cgcaatgccg tcatcgatct cgatctgacc accatgaccg gcgaagtttt
     7681 gccggtgcgc ttcatgcacc gcgtctccgc aagccgtgaa ggcatcggcg ggccgacgcg
     7741 aacgatcgtt ctcaaccgaa cccagggcga ggatgcttcc gccgatctgc gcgcctccga
     7801 ggttcggttc acccgtttct tcaattcgac gccgatggcg atagccggcg tcgaccaggg
     7861 cgggcgcatc ctgcgcacca atgcgccctt cctttcgctg ttttcttccg ttgtcgaccg
     7921 cgatgcgctg gaccggcgcg tgcggctcga cacggtgatt cacgagcgag acagggcggc
     7981 ctttgccaca gctttcgagc gggcgcgcca gcggcaggcc gacatcgcgc cgatcgatac
     8041 ggtgctgccg ggcaatgacg agcgccatat ccgcttttac gtcaacgccg tcgccgacag
     8101 cgcggcaggc gagggggcgg aagaagcggc gatcgtctat gcggtcgaga ccaccgagca
     8161 aaaggcactt gagggccaga tggcccaaag ccagaaaatg caggccgttg ggcaactggc
     8221 cggcggcatc gcccacgatt tcaacaatgt gcttaccgcc atcatcatgg cgtcggactt
     8281 gttgctgacc aaccatcgcc catccgatcc atccttcccc gacattatga acatcaagca
     8341 gaatgcgaac cgggcggcct cgctggtccg gcagcttctg gcgttctcgc gcaaacagac
     8401 gctgcggccg gaggtgctga acctgactga tgtgatggcg gacctgcgca tgttgcttgc
     8461 tcgtctggtc ggcaacgaca tcaagctcaa ggtcgagcat ggccgcgacc tgtggccggt
     8521 caaggttgat cttggccagt tcgagcaggt catcgtcaat ctgaccgtca atgcgcgcga
     8581 cgcgatgccg acgggcggcg aattgatggt gcggacccgc aatgtcacgg ctgaggaaag
     8641 caggaacttc tcctaccgcg aattgacgtc cgccgactat gtgctggtcg agacggaaga
     8701 tacgggaagc ggcataacgc ccgaagtcct caagaagata ttcgagccgt ttttcaccac
     8761 caaggatgtg ggcaaaggaa ccggcctcgg cctgtccatg gtctacggca tcatcaagca
     8821 gaccggtggg ttcatcttct gtgactccga ggtcggcaag ggaaccgttt tccgcatctt
     8881 cctgccgcgc tatgtcgctg aggtgcgaac atcgtcggaa acgggcctgg atgtgcccgt
     8941 cgcggcggcg cccgttccga aaatcgccga cgcggcaaaa gacctgtcgg gttcggccac
     9001 cgtattgctg gtggaagacg aggatgccgt gcgcatgggc ggcgtgcggg cgctgacctc
     9061 caggggctac accgtacatg aagcctcgtc gggcgtggag gcgctggagg tcttcgccga
     9121 acttgagggc aaggtcgata tcatcgtctc ggacgtggtc atgccggaaa tggacgggcc
     9181 gacgctgctc gtagagatcc gcaaattgca gccggacatc aagttcgtct ttgtttcagg
     9241 ctatgcagag gacgccttcg ccaggaatct gcccgccgac gcccagttcg gtttcctgcc
     9301 aaaaccattt tcgctgaaac agctggcaac cgtggtcaag gacgtgctgg aatcatagct
     9361 ttccagccgt cccgccaatg gtgcaggtcg aagccgtgaa actgccgttc ggctggcgca
     9421 ggatgatgtc gaacgacgcg tcatgctggc catcggctgt cacctgcgtc agcgaaacgc
     9481 tgttgccgcc cgccgtatag ccaccgagtg gccccaacca gctgatctct ccccgaggcg
     9541 atatttggta attgtagccc tgctgcatcc cgccaactat cgatggaccg ctatagacgt
     9601 cacccttgat cacgatctca cccagataaa tgaacatgcc cagcatgtag acttcgcagc
     9661 ggtaggcgcc gtccggcagc acgccgcctc cggcgctcgc cgtcgccggg ccggacaggc
     9721 tggcaagcag cggtagcgcc agaaaaacaa gccgtcgagg catcatgagc gtgcttccag
     9781 tcgtgctgaa cggcctgatt ctcaagccaa tgacctccgt cgtcaacagg cccggtccac
     9841 gcacccggca ttggcttgcg gcgggccggt tgaacaggag ttcccttcag cgccggcaag
     9901 ttccgcacaa gcaaacgctt ctagcttccc aagggtgagc gtcagtggtg tgttggcaat
     9961 ttccgggggc gacgatgggg cattttgtcg aggactacca gaaacggcgg cttaccgagc
    10021 gcgtcgacat tctgagcgcc gtcaacattt tgaagtccaa gggctacgac aatgacgacc
    10081 tgatcacgga aatcatccgt gtcttctatg tcgacctgga cacctataat gacatcgtaa
    10141 aggcagccta gggaaaggcc gctatccggt tcgtccagcg gccaaatcgt tcaaatgcgg
    10201 gaaatcaccc agcgctgtcg tcgctcaact gctgcgaatg accgtcgatc acgccaaaat
    10261 cctgcgactt ggagccgtta tcggcggtca ggaagccatc tcccgccaat ccgcgtcgta
    10321 aaagctcccg cacagccgct gcgcggctgg gcatccgttt gtcaaaacgc cagttctcca
    10381 gcgctaacaa ttcgtcggcg ctcaacatga tttgcaaacg ttccgggcgt cccagatcga
    10441 ccatggcgaa ttcctcacaa ttcggcttag gctacggtga atgagtaagt gacctattct
    10501 gataattctt ggcacaagcc ctatctgtgt tcaagtggtt tttaagcgcg ctatgcgaca
    10561 atggcgcaga atggatggat ggccgcatcg ccgccaagac gggtatgtac taactatatg
    10621 aatctaaaag ataaaatgga gaaaaccttg ccaatttcgc acggcggtgt atggtagtcg
    10681 caggacaggg gaacgacgcg cagcttattg cgaccgacag cgaggtgttc cgatggacgt
    10741 acagctttca ccacaaattc tggacgatat gacccattcg cttcaggcgg ctttggcgaa
    10801 aaacggcatc atcaacatac tgcaattggc tgaggaaatt cgccggcgca acgaagtcga
    10861 gaatatcgcc ttggaagacg ttgccgcaaa gctgatgtta aaggcacagc ttttcaacgc
    10921 cgcgatggag ttcgacagcg aggcagatat ttctccgctc gcaaaatagc cggaacgttc
    10981 attggccgta caggaaacgc cccggagcac ctcgtgcttg gtcaggcgtt ctgcttcgcg
    11041 gagtggtcag gccatccacg cgaagggtcg cgagcgggga tggggactat cgggtcgagc
    11101 gatcagtcaa agcgaaggcc cacggcccgg aaacccaatg accggttccg gtcaagccca
    11161 agtaccttcg tctcgaccgt gccaccggtg cgattctcga caaaagcctc gaccttgctc
    11221 aggccaccgc cggcggaatg cttccctctg accggaatgc ttccatccaa ccaggaagcg
    11281 ctttcgatca caacggaatg ttgtcgtgct tcttccaggg gttctgcaaa tccttgttgc
    11341 gcagcatgcg cagtgcccgc gcaatgcgcc ggcgggtgga gtgtggcatg ataacttcgt
    11401 cgatgtagcc gcgctcggcg gcgacgaaag gcgacaggaa gcgatcctcg tagcctttgg
    11461 tatgcgcggc gatcttctcg gcgtcgccta tgtctttgcg atagatgatc tcgaccgccc
    11521 ccttggcgcc catcaccgcg atctgggctg tcggccaggc ataattcatg tcgccgcgca
    11581 ggtgcttcga cgccatcacg tcataggccc cgccataggc cttgcgggta ataaccgtga
    11641 ttttcggcac cgtcgcctcg ccataggcga acagcagctt cgcgccatgc ttgatcaggc
    11701 cgccatattc ctgcgcggtt ccgggcagga agcccggcac atcgacgaag gtgacgagcg
    11761 ggatgttgaa acagtcgcaa aagcgcacga aacgcgccgc cttgcgcgag gcatccgaat
    11821 ccagcacgcc cgccagcacc atcggctggt tggcgacgaa gccgaccgtg cggccttcga
    11881 tgcggccgaa gccggtaacg atgttcttgg cgaaggcctg gctgatttcg aagaaatcgc
    11941 cttcgtcgca ggtcttcagg atcagttcct tgatgtcgta aggcttgttg gcgttgtccg
    12001 gtatcagcct gtcgagcgaa agatcgtggt cggtgaccga ttggtagcac tcgacctcgg
    12061 gaatttccgc cgtgttggag gcgggaagaa gatcgaccag ccgccgcatt tgcaacaatg
    12121 cttccacatc attgtcatag gcgccgtcgg cgatcgagga tttggtcgta tgaaccgatg
    12181 cgccaccgag gctttcggcg gtcaccgtct cgttggtcac ggtcttcacc acatccggcc
    12241 cggtgacgaa catgtaggag gtgtcgcgca ccatgaagat gaagtcggtc atcgccggtg
    12301 aatagacgtc gccgcccgcg catggcccca tgatgaccga tatctgcgga atgacgcccg
    12361 aggccaacac gttgcgctgg aagatttcgg cgtagccgcc aagcgccgcc acgccttcct
    12421 ggatgcgcgc gccgccggcg tcgtagatac cgatgatcgg cgcgcggttg cgcagcgcca
    12481 tatcttggac cttctggacc ttttcggcat gcgcttccga cagcgagccg ccgaataccg
    12541 tgaaatcctt ggcaaacaga tagaccggcc ggccgttgat cgtgccccag ccggtgacca
    12601 cgccatcgcc ggcgatcttc tggctgtcca tgccgaaatc ggtggagcga tgctccacga
    12661 acatgtcgaa ttcctcgaac gaatcttcgt cgaggaagat ttcgatgcgc tctcgggcgg
    12721 taagcttgcc cttcttgtgc tgggccagga tgcgggcctg cccgccgccg gcgcgcgcca
    12781 cttcgcggcg gcgttccagt tccttcagca catctttcat cgaaaggccc ccaggcaata
    12841 tttgcccctt ggtacttagc tgggcaggag agtgcaagcg tcgttgcgcg tgaagaaatg
    12901 ttgcggtgca gcatgacgcc tcttgccttt atgcgcgaac tggcccatat gcagccaata
    12961 ggcgcttggg ccggaattta ccggccttct ctacaagtga gactggttgc gtctgttttc
    13021 tctctttccg atttacggaa agcgatgaaa aagccccata ggcatgaagc ctgcgggcac
    13081 gacagcgcag ccgcgcgggc cacacgcccg ccgccttgtc gtttcctgaa ttttgtcgac
    13141 ggcaggttgc gccctgccgc cagttgtttg cggccgtcga gccgcacgaa agaagataat
    13201 tttgactaat caaaacaccg cggtggcgat ggcttccgca gaccacgaca cagatcaact
    13261 gaccggcttt gccgcgctcg gcatttcggg cgtgctgctg aaggccaccc atgccgccgg
    13321 cttcaccgaa ccaaagccca tccaggtcca ggcgatcccg ccgcagctgg agggccgcga
    13381 cattttcggc atcgcccaga ccggctccgg caagacggcg gcgttttcgt tgcccattct
    13441 caccaagatc atggcgcttg gcaccaagcg ccgcgccaag acctgccgtg cgctgatttt
    13501 ggcgccgaca cgcgagcttg ccgtccagat cgaggatacg atcaagatcc tcgccaaggg
    13561 cgctcatgtc tcgaccgccc tggtgctcgg cggcgtctcc cgcttcagcc aggtcaagaa
    13621 gatcgccccc ggcgtcgata tccttatcgc gacgcccggc cggttgaccg acctggttcg
    13681 tgaaggcgat ctcgtgcttg ccgatacgaa atggctggtg ctcgacgagg gcgaccgcat
    13741 gctcgacatg ggcttcatca acgacgtcaa gcgtatcgcg cgcgctaccg ctcccgatcg
    13801 ccagaccgcg ctgttttcgg ccaccatgcc cgacgagatc gccgagctgg ccaagggcct
    13861 gctgaagaac ccgatccgta tcgaggtttc cccgcagagc acgacggtgg ctgaaatctc
    13921 gcaaggcgtg atcttcgcgc gcaccaagca gaagcggcag gttctgtcga ccatgctggc
    13981 cgatgaggcg atgaagtccg tcatcatctt ctcgcgcacc aagcatggcg ccgaccgcgt
    14041 caccaaggat ctggagcgtg atggcttcaa ggccgcggtg atccacggca acaagtcgca
    14101 aaacgcccgc cagaaggccc tgagcgactt ccgtgaagga tcggtacgca ttctcgtggc
    14161 aaccgacatc gccgcgcgcg gcatcgacgt gcccggcatc agccatgtgg tgaatttcga
    14221 cctgcccgac gaagccgaaa gctacgttca ccgcattggc cgcaccggcc gtaacggccg
    14281 cgacggcatt gccgtcacct tgtgcgatcc gtcggaaaac gccaagcttc ggcaggtgga
    14341 acgcattatc cgcgccaagc ttccgatcgt cgccgaccat ctggccagcc ccgatccggt
    14401 gcgcaacccg gctgaaaaga acgagcgcga atatgttgat ccccgtcgcg tcaagcgtcc
    14461 gggcggcgcc aaccagaaca acagcaagcg cttcggcgac aagcccacgg gcgagcgcaa
    14521 ggccgatggt gcaaagccgc cgttcaaggg caacaacaag cgccgctttg gcggcaagag
    14581 gccggccgcg cgcgccgcat aggctgtgct gagcgaaccg gtttcgcttc ctgaactagc
    14641 agactatcga gggcggccgg agcaatgttc cggtcgcctt ttctttgccc tggccgagta
    14701 tcgacgcgca aaaatattac gtcttgaggc gagatctcgt agcaccagtc gcaaatgcgt
    14761 ctggtccagg tgatttcctg ccttgaaacg ccgcgccgac atttctaggg ttttgcgaaa
    14821 gtctggaagg atagtctcat ggttggttcg gtaatcgacg cgatcggcaa tacgcccctg
    14881 attaggctga agcgggcgtc gcaggagacg ggctgcgaaa tcctcggtaa ggccgagttc
    14941 atgaatccgg gtcagtcggt caaggatcgg gcagggctgt tcatcatccg cgatgccgaa
    15001 aagcgtggcc tgctcaagcc cggcggcgtc attgtcgagg gcacggccgg caatacgggc
    15061 atcggcctga cgctggtggc aaaggcgctc ggctatcgga cggtcatcgt catccccgag
    15121 acccaaagcc aggaaaagaa ggatacgatc aagctgctgg gcgcagaact gatcgaagtg
    15181 ccggctgtgg cctacaagaa cccgaataat tacgtgaagc tgtctggaag gctggccgag
    15241 cagatggccg ggaccgaacc gaatggcgcg atctgggcca accagttcga caatgtcgcc
    15301 aaccgtgatg gccatatcca cacgacggcg caggaaatct ggcagcagac cggcggcaag
    15361 gtcgacggtt tcgtttcggc ggtcggttcc ggcggcacgc ttgccggtgt cgcggccggt
    15421 ctgcgcgaca agaacaggga cgtgaaaatc gcgctggccg acccgctggg ggccgcgctc
    15481 tataatttct atactgccgg cgaactgaaa tccgagggca gttcgatcac cgaaggcatc
    15541 gggcaggggc gcatcaccgc caatctggac ggctttgccc ccgatttttc tttccaggtg
    15601 cccgacgacg aagctttgcc gattatcttc gaccttatcc aggaggaggg gctgtgcgtc
    15661 ggcggctcga cgggcatcaa cattgccggc gcgatccggt tggcgcgcga attgggaccg
    15721 ggccacacca tcgtgaccat cctgtgcgat ttcggcacgc gctatcagtc caagcttttc
    15781 aacccggcat tcctgcgcac caagaacctg cccgttccgg gttggatgga gcacaagagc
    15841 gagatttccg tgcctttcga agcggcggca tgagcgtgcg caagacagaa atactgttcc
    15901 gcgacgattc ctacctcaag accgcaaccg cgcgtgtcga ggcgatcaac gagcgcggcg
    15961 gcatcatcct cgataggacg attttctatg ccacttcggg tggccagccc ggtgacacag
    16021 gctttctgga gcgggctgat ggaagccgca tcgccattag cgcaaccctt acgggcgaga
    16081 ctaaggacga gatcatccac gttccgtcac ccgaccagcc gatcccggcg gtgggtgagg
    16141 aactgaaact cgtcatcgat tggccgcgcc gccatctgtt gatgcgcatg catgccgcct
    16201 gccatctgct gacggtggtt tgtccgtttc ccatcaccgg cgcggcggtt tcgcaggacg
    16261 attcgcgcgt cgatttcgac atgccggatt tttcgatgac caaggaagaa gtaaccgccc
    16321 ggctgatgga actggtgcgc gccgatcatc cgatcttcat ccgctcgatc accgaggagg
    16381 aactggcggc aaatcccacg ctggtcaaat ccaagaatgt gcgcccgccc acgggaacgg
    16441 gtcacatcag gctggtctgc atcggagaaa atgccagcat cgacagccag ccctgcggcg
    16501 gcacgcatgt gaaaagcacc ggcgaggtcg gtgaaatcca catcggcaag atcgagaaga
    16561 aaggtcgcga gaaccgccgc tttcgcattc ggttcggggc gttgccggct gcctgagtta
    16621 aggctacggt tttcaaccag acgcatcgtc gggagaagca agatggcgca agacagtccg
    16681 tttaccgtcg atgccgactg gttgcaggaa aagcttggcc aaccgggctt cgccattgtc
    16741 gatgcgtctt ggtacctgcc tgcgcaaaac cgcgatgcac gggcggagta tgaggcggcc
    16801 catattccgg gcgcggtatt tttcgaccag gacgcggttt cggatcgaac ttcgccgttg
    16861 ccgcacacgc tgccttcgcc cgaggatttc gcccgctatg tcggcgccat gggcatctcg
    16921 gccgatgaca ccatcattgt ctatgacggc cccggtttct tttccgcgcc gcgcgcatgg
    16981 tggatgttca gggtcatggg cgtcttccag gtctatgtgc tcgaaggcgg actggaccgc
    17041 tggaaagcag caggccggcc ggtgaccgcg cagacgacca aggttgcgcc atgcatcttc
    17101 cacgccgatt tcgatagctc gcgcgtcgcc agccttgccg acatgcgcca catcgtcgat
    17161 ggccacgaga gccagattgc cgatgcgcgt ggtcccggcc gttttgccgg tactgaaccg
    17221 gaaccccgcg ctggcatccg gtccggccac atgcccggcg ccaggaacgt tccggcatcc
    17281 atgctgtcgc aggacggcca acttctgggc aaggacaggc tgcgcaaaat cattgtcgat
    17341 gccggcatcg atctttcgag gccggtggtc acgtcgtgcg ggtccggtat caccgccgcc
    17401 gcactcacct tggcgctgga aacgattggc cacacggata accgtctcta cgatggttcg
    17461 tggacggagt ggggcggtct ggccgatacg ccggtcgcca cgggcaagga atagcatggt
    17521 gaatagcagt cagccggacg atatcgaggt gaccgtcacc ttcctcgaaa tggaaacgcc
    17581 gccggcctat tacccggctc tgccctataa ccggcagatc gccttgttga aggcaaagga
    17641 cattccgttg catttctacc gctatctgac cgaccgggtc gggcgcaaat ggcattgggt
    17701 caatgtgctg cggcttgacg acgacgaact tgccgccagc cttcaccgcg aggaccgcga
    17761 tatccgcatt ctctatctgg acggtgcacc ggccggctat ttcgacgtca agccgcattt
    17821 gccggatgaa acggaactcg cctatttcgg catgatggac catgcgatgg ggcagggcct
    17881 tggccgctgg ttcctcggcg cggcgatctc ggcttgctgg gcgcatggac cgaagcgggt
    17941 gaccgtgcag acctgcacgc tcgaccatcc ggccgcattg cctctctacc agaagcttgg
    18001 cttcacgccg gttgggcaga agaaagaact tgtgcgtccg atgcccttcg ctgagcgctc
    18061 ggcaagcgtc atgcgcccct gacggctggc tgaatggcgc gttcaccctg gcttcagctt
    18121 tcctttgcga tgttgcgtgc gaggccagga cgccgcgcat tcatatcgaa ggagttttgc
    18181 tgcatgataa cccgccgagt tttcgccgcc gccatgatcg cggcattggc cgtgccaggc
    18241 atggctttcg cgcaagcgcg aacgccgtcc ggcgagcaga tcgaacgacg gctcgaaatt
    18301 tcgcctcgca tgaggctgcg gccgaaccag cgcgtcacca tccaggaatt caagcggcgg
    18361 ccggacctgc ggcgtgcggc gccctcgatc gatatccagt cgataaattt cgcattcggt
    18421 tccgccgaaa tccctaactc gcaatatgac aaggtggaga acatcgcgga cggtcttcgc
    18481 cgcatccagc gccgccagcc gcgcgcccgc gtcctgatcg aaggccacac cgacgcggtc
    18541 ggttccttcg ccgcaaacca ggcgctgtcg caagatcgcg ccgcctcgct gaagcgaacg
    18601 ctggtgcgtg agttcggggt tccggcaagg atgctggaga cggtcggtta cggcgaggaa
    18661 ttcctgttgg tgccgacgca aagcgagaac tggcaaaacc gccgggtaac gctgcgccgg
    18721 ttcgacgatt tcatccgtta atcgctcgtt aaccaaaact gcgcattatc gttctcactt
    18781 gtcacgctat cgtcgcacag gcacggtatc agacaagtgt ggaacgattt gccgaccgcg
    18841 gatgaaccgg cgggaaatcg cgatagacga ggaagcgggg ttctgccccg cttttttgtt
    18901 gcccaaattc ttccgccgac catttgacgc gaaaaacccg ccgggttgag ccggcgggtc
    18961 tttcgcaaag cgtgtcgagg tcaggctgcg agcacggcct tcggctcgga caattcgtac
    19021 ccgagcgcat cggcaaccgc gcggttggtg atcttgcctt tgtggacgtt cagcccgttg
    19081 cgcagatggc tgtcgtcgat cagcgccttg aggcccttgt cggcaagctg tagcccgtaa
    19141 tgcagcgtcg cgttgttcag cgcgtgggcc gaagtcaccg gcacagcgcc cggcatgttg
    19201 gccacgcaat agtggatgat gccatcaacc acataggtag gatcggcatg cgtggtggcg
    19261 tgcgatgttt caaagcagcc gccctggtcg atggcgacgt cgacgagcac ggcacccttc
    19321 ttcatgcccg aaagcatttc gcgggtgacg agcttgggtg ccgtggctcc ggggataagc
    19381 acggcgccaa taacaacgtc cgccgagaag cattcttcct caagcgcctc gaccgtcgag
    19441 tagcgcgtat ggacgcggcc gttgaaaatg tcgtcaagct ggcgcaggcg cgggatcgag
    19501 cgatcgatga tgctgacgtc tgcgccaagg ccaaccgcca tgcgcgcggc atgcaggcca
    19561 acgacgccgc cgccaagcac ggtaactttg ccgggcagca cgcccggtac gccgccaagc
    19621 agcacgccgc gaccgccatt ggccttctgc aaggcggtag caccggcctg gatggaaagg
    19681 cggccggcca cctcggacat cggcgccagc agcggcaggc cgccgcggtc atcggtcacg
    19741 gtctcataag caacggcggt gacgcccgac gccaacaagc cttttgtctg ttccggatcg
    19801 ggcgcgaggt ggagataggt gtagaggatc tggccttcgc gcaactgcgc ccattcgccg
    19861 ggctgcggct ccttgacctt gacgatcatg tcgcattgag caaacacctc ggctgccgtc
    19921 ttggcgatgg ttgcgccggc cgcgcggtag gcgttgtcgt cggcgcctat gccttcaccc
    19981 agcccgcgct cgaccaacac ttcatggccg tgcgccacat attcgcgaac cgaaccgggt
    20041 gtcagcccga cgcgatattc atggttcttg atttccttga ggcagccgac acgcatttct
    20101 tatcctccgc ttgaccgtta ttgccagttc caggcaaggc ggagtcaacc acgaaaccag
    20161 gcgcatgtat ttgcgaattg tcttgttaaa gcgccgtcgt atcgataatg gttgcgccaa
    20221 aatacgatat attcgcagga atcgtcaaaa atgccgctcg ataggatcga tatcgctatt
    20281 ctcgatgcct tgcagaaaga ggggcgtttg cccaacgcgg ctcttgccga gaaggtcggc
    20341 ctgtcgcagt cggcctgttc gcgccggctc gacaatctgg aaaaatccgg cgccattcgc
    20401 ggctatcatg ccaggctttc caacgcggca cttggccacc agatgacggc gatcgtccat
    20461 atatcgctgt ctggccagtt cgaaaagacg ctgtcggact tcgaggcggc gataaaaaga
    20521 tgccccaatg tcttgtcctg tcatttgatg tctggagagt acgactacgt tttgcgcatc
    20581 gcgtcgcggg acctggcgga ttatgaacgc atccacaagg aatggctgtc ggcgatgccg
    20641 catgtgacga agatcaattc gtcttttgcc ttgcgcgaaa tcgtcgaccg cacggccatg
    20701 ggcttgaagt ccgacatggc ttgacgcccg gcatcgcaac tgaacctatc gcccgcagcc
    20761 aacatgtcag gatcaaagca tgcgttatgt catcattatc gctgcaattg cgttcttcat
    20821 catctgggat ggcctcttca acgaaggccg ctatctcgac cattcagttc gtgaagtgaa
    20881 aaccgtgatg cgcgacatta cgggttgagc gtgccgggcc gccgggcgcc gtcgcagttt
    20941 gtggcgcgac atcctgccga ttgacgcggc cggatcgctg acggaaaagg ctgccgtcga
    21001 accatcgatc gcgcgccttg ccgtttagcg cggcgcggga caaggagact gcattgatcc
    21061 ggcatatcgt tttcttcagc gccaagcata aggatgaagt cgaggcgatt cgcttcggcc
    21121 tgatggcgct gggcaagatt ccgcattcgc agctgtttga ggtgtcgctg aacagcaagg
    21181 tcgatccatt tggcgatgaa atcgacgtcg tggtctatgc cgaattcgaa gatcacgccg
    21241 cgctcgccgc ctataaggcc catcctctct acgccgagac gacggcaaag gtccgcccga
    21301 agcgagaact gcgcttctcc gccgacgtgc ccgccggcgg ctgatccatc ggtcaggccg
    21361 ggcaagcgca agtcagccca cgaaggaaaa agtgaggggg gcgtgatgaa tgccacccac
    21421 cacggctctg acctacaatc gtctttcgcg cattcccggc taaaatccga cgaaacctcg
    21481 agcggcttat aggattagac aaataattcc agtcaccgaa ttcccgatgc tttttttacc
    21541 gtcgctaatt atttgaaaag aatggctccc cgggccggat tcgaaccagc gaccaaccgg
    21601 ttaacagccg gttgctctac cactgagcta ccggggaacg ggggctcttg tcgtgcgagg
    21661 cagcctatag caaacccgtt tcagctttgc aaagaagcat ttcgcttttt tctcggtctt
    21721 ttttcggcca atggtcgcaa tctcccattg gcgcctctta tatagcagct tgccgtactc
    21781 atggcggacc tcattgccgg aaagcgatcg atctggaaaa gccggataag aagttgaata
    21841 tgaccgcagg aaatgacggc cacgctgcgc cggcacgcaa gatttccgtt ttcggccgcg
    21901 agttcaaaat gccgcggtcg cgctgggcgc gaatcgccat tggcttggcc ctgaccattc
    21961 tcggcctctt cggtttcctg cccatcctgg gcttttggat ggtaccgctc ggattgctgg
    22021 tgctttccta tgagtttgca tcggtgcggc ggctcaggcg tcgtttcgtc atctggtggg
    22081 ggcggcggcg caggcggtcc taggctgcgt aatggcgggc gcgggtgcgt ccgcgatttt
    22141 ccagcgcgtg gttcagcctt cgatcggttg tggcgaagat ggcttttttg tttgcgcccc
    22201 ttgaaggact gaaaaagaac gtctattc
//
LOCUS       DASYCS010000031         5287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2613740,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000031 DASYCS010000000
VERSION     DASYCS010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 5287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5287
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2613740"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="VGM46_01335"
     CDS             <1..560
                     /locus_tag="VGM46_01335"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY4191263.1"
                     /translation="FDGENVTGLRPYKLNRRGLARTFQLARPFSQLTVLENAMVGSYR
                     LSRHKREAAERAREVLALLGIEADADRPAGELTMPGLKRLEVARALATRPKLLLLDEV
                     MSGLTPTESKAMLPVIQRIRDSGVTLLMIEHVMHAIMTLSDRIVVLHHGRKLAEGTPA
                     EIAADAAVIEAYLGAAPIGKDGSHA"
     gene            553..1272
                     /locus_tag="VGM46_01340"
     CDS             553..1272
                     /locus_tag="VGM46_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4191264.1"
                     /translation="MPEPLLKVEGLHAGYGNVSVLHGIDFQMNAGEVAALIGANGAGK
                     STTLRCLSGLLRPTSGTIRFAGEVVSGLAPHRTVEMGLVQVPEGRLLFPAMSVEENLM
                     LGAFLPRPRKVMAQTLESVLDYFPALREKLGVRAGSLSGGQQQMVAIGRALMAKPKLL
                     VLDEPSLGLAPLVVRDIFTNIEKLAQSGLAILLVEQNIGNALSLAHQAWVLENGVIVT
                     RGSGSELMTDPKVQAAYLGLH"
     gene            1464..2006
                     /locus_tag="VGM46_01345"
     CDS             1464..2006
                     /locus_tag="VGM46_01345"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEY4191265.1"
                     /translation="MSRLIDIVITDRSIEGRGDLPDTVRPGEFTKGGETVIVEAITIV
                     TLFVMVATTLIGVLDRFVFGFGLPWPEELARFLLIWSSLLAAAVAAKHQSHFRLTFLS
                     DRFGPKWTDFMDVVCVIAVLVVAWKGVEFAMIFNTQTSPALGLPMSYVYASVPVSSVL
                     IAFYIVQNLLARHRSAGKAG"
     gene            2013..3293
                     /locus_tag="VGM46_01350"
     CDS             2013..3293
                     /locus_tag="VGM46_01350"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEY4191266.1"
                     /translation="MLLFLILFLVTMALGFPIAFAMLLASLYFIVSDSGSDLIIVPQQ
                     IQDGLNSFPFLAIPFFVLAGNLMAEAGVINRFVQLAQSLIGHVRGGLAHAVILSGALL
                     SGVSGSGTADAAALGSTMIPALEKQGYKKDFAVVLCAFSGALGPIIPPSIFLIIYGAM
                     GNVSIGQLFLAGVVPGILMVLVLMATSHIVVTRKGYGVQMPKTTLAEKGLALKHAAFD
                     LVLPLGIIGGIRFGIFTPTEAGAVAVLYVLFIGLVVQRSLSFAQILKAARETVDVLGA
                     VMLLIAMATIVNYILALFQASDHLAAAVVGLSSNPIMFLILTNLLVIFLGSIIEDTAV
                     LVLMTPILVPLLDNFHIDPVHFGMVLCMNLTIGLVAPPVGLAMFVTCSIGQISIERFM
                     RESWPFLLALLLLLLALIVFPSISLWLPHQMVHV"
     gene            3290..4345
                     /locus_tag="VGM46_01355"
     CDS             3290..4345
                     /locus_tag="VGM46_01355"
                     /inference="COORDINATES: protein motif:HMM:NF025694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate cyclase domain-containing protein"
                     /protein_id="HEY4191267.1"
                     /translation="MSRSPLLEQIGWNVDRLVTTDFPSYGVIGPLYRGAVALQGGPLC
                     MRAAEMLKENVAPGDTVILLTGLILPGHYPYGETDGPLGAAVLARALAIGLKARVLVV
                     VEPELTGLMGALLRVAELPFVTLAALETESGLHRPAAAVVGLSRDSATAHADARSWIG
                     SLNAKAVVAIEKAGTNAQGVYHMVGGADISATVGKGDAVFSVAAELGLVRIGIGDRGN
                     ELGTGPIAEVTRALLPFGTDCGCPCHGGVADQTPADLAVPAVISNWGAYGVAACLAAL
                     HEQPDLLHSASLERRLTETAILNGGVEGMSGRAKLAVDGIGVDTSAAMAELLVEVYRA
                     QSVRDPSPFSTPLIKKQ"
     gene            4368..>5287
                     /locus_tag="VGM46_01360"
     CDS             4368..>5287
                     /locus_tag="VGM46_01360"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEY4191268.1"
                     /translation="MITRRTMLAGTCLAGTSLAGLLSGLKLAQAAEFTFRVASVLEAR
                     SDLYGGFDHFAKRTSELTNGRVEVTTYKNTLGKGERQLFEAVQLGTIEGTVATTGTLS
                     AFVPEADLFNLPFLFKNTAHAFKVVDGEIGDEFNKKMIAKGFRILGWWVTGTRNTSNN
                     VRAINSPDDYKGLKIRTMESPPFIELYKSMGAIPVPMSISEVYTGLSQGTIDGIDGSL
                     PAQLEFKHIEVLKYAAVTDHVVLLYAFVVSERWWQQLPEDVHAAIMKAEAEGRDIERK
                     DDEAYNKKVEAEWVKEGKTVTHPDIKKFQD"
BASE COUNT          888 a         1653 c         1709 g         1037 t
ORIGIN      
        1 tcttcgatgg cgagaacgtc accgggctac gcccctacaa gctcaaccga cgcgggcttg
       61 cgcgcacttt ccagctggcc cggccgtttt cccaactgac ggtcctggag aacgccatgg
      121 tcggcagcta tcgcctgtcg cggcacaagc gcgaggcggc ggagcgggcg cgcgaggtgc
      181 tggcgttgct cggcatcgag gccgacgcgg accgcccggc cggcgagttg accatgccgg
      241 gcttgaaacg gctggaagtg gcgagggcgc tggccacccg gcccaagctg ctgctgctgg
      301 acgaagtgat gtcgggcctg acgccgacgg aaagcaaggc gatgctgccg gtgatccagc
      361 gcatccgcga cagcggcgtg accttgttga tgatcgagca tgtcatgcac gccatcatga
      421 cgctgtcgga ccgcatcgtg gtcctgcatc acgggcgcaa gcttgccgaa ggcacgccgg
      481 ccgagattgc cgccgatgcg gccgtcatcg aggcttatct gggcgcggcg ccgatcggga
      541 aggatggatc tcatgcctga accgctgctc aaggtggaag gcctgcatgc gggatatggc
      601 aacgtatccg tgctgcatgg catcgatttc cagatgaatg cgggcgaggt cgcagcgctt
      661 atcggcgcca acggagccgg caaatcgacg acgctgcgct gcctgtcggg cctgctgcgg
      721 ccaacttccg gcacgatccg ctttgccgga gaggtggtga gcggccttgc cccgcaccgc
      781 accgtcgaaa tggggctggt gcaggtgccg gaaggacgcc tgctatttcc cgccatgagc
      841 gtggaagaga atttgatgct gggcgccttt ctgccgcggc cgcgcaaggt catggcgcag
      901 acgcttgaaa gcgtgctcga ttattttccg gcgctgcgcg aaaaactcgg cgtccgggcc
      961 gggtcgctca gcggcggcca gcagcagatg gtggcgatcg ggcgcgcgct gatggccaag
     1021 cccaagcttc tcgtgctgga cgagccgtcg ctggggctgg cgccgctggt cgtgcgcgac
     1081 attttcacca acatcgagaa actggcgcaa agcggcctcg ccattcttct ggtggagcag
     1141 aatatcggca acgcactgtc gctggcgcat caggcatggg tgcttgaaaa cggcgtcatc
     1201 gtcacgcgcg gcagcggcag cgagctgatg accgatccca aggtgcaggc cgcctatctg
     1261 gggcttcact aaagcctgtc cataagcgaa aaccgcctgt ggcggcgaag cccggcgaca
     1321 tgcgttcccg gcgacgacgg gcggtcgccg cctgcgtttg cctgcgcttc atcggcgttt
     1381 gacgccctcc aaatccctgc ttgccaccgc ttttttcctt cgattctagc cccggaagcg
     1441 ctcatttaga atttgcgctt gaaatgtccc ggctcattga tattgtgatc acagatcgca
     1501 gtattgaggg acgcggcgat ctgccggaca ccgtccggcc aggggaattc acaaagggag
     1561 gagagaccgt gatcgttgag gcgattacga tcgtgacgtt gttcgtcatg gtcgcgacga
     1621 cacttatcgg cgtgttggac aggttcgtat tcggcttcgg gctgccgtgg cccgaggagc
     1681 tggcgcgctt ccttctcatc tggtcgtcac tgttggcggc ggcggtggcg gccaagcatc
     1741 aaagccattt tcgcctgact ttcctgtccg accggtttgg gccgaaatgg accgacttca
     1801 tggatgtcgt ctgtgtgatc gcggtgctgg tcgtcgcctg gaaaggcgtc gaattcgcga
     1861 tgatcttcaa tacgcagact tcgccggcgc tgggcctgcc catgagctac gtctatgcct
     1921 cggttccggt gtcgtccgtg cttatcgcct tctacatcgt ccagaacctg ctggcgcggc
     1981 atcgatccgc cggcaaggcc gggtgacggg ccatgctgct tttcctcatc ctgttcctgg
     2041 taacgatggc gctgggtttc cccatcgcct tcgccatgct gctggcctcg ctgtacttca
     2101 tcgtttctga cagcggcagc gacctgatca tcgtcccgca gcagattcag gacggcctca
     2161 actcgtttcc cttcctggcc attcccttct tcgttctggc gggcaatctg atggcagaag
     2221 ccggtgtcat caatcgcttc gtgcaattgg cccagtccct tatcggccat gtgcggggcg
     2281 ggcttgccca tgcggtgatc ctgtccggcg cgctcctgtc cggcgtatcc ggatcgggca
     2341 cggccgacgc cgccgccctt ggcagcacga tgatcccggc acttgaaaag cagggctaca
     2401 agaaggattt cgccgtcgtg ctgtgcgcct tttcgggcgc cttggggccg atcattccgc
     2461 ccagcatctt cctcatcatc tacggcgcga tgggcaatgt ctcgataggg cagctgttcc
     2521 tggccggcgt cgtgccgggc atcctcatgg tgctggtgct catggcgacc agccatatcg
     2581 tcgtcacccg caagggctat ggcgtccaga tgccgaagac gacgctggcg gaaaagggcc
     2641 ttgcgctgaa gcacgcggcc ttcgaccttg tcctgccgct gggcatcatc ggcggcatcc
     2701 gctttggcat cttcacgccg accgaagccg gcgcggtggc ggtgctctat gtgctgttca
     2761 tcggcctggt cgtccagcgc agcctgtcgt tcgcgcaaat cctgaaggcg gcgcgcgaaa
     2821 ccgtcgatgt gctgggagcg gtgatgctgc tcatcgcgat ggcgaccatc gtcaactaca
     2881 tcctcgcgct cttccaggcg tcggaccatc tggcggcggc ggtggtgggc ctgtcgtcca
     2941 acccgatcat gttcctgatc ctgaccaacc tcctcgtcat cttcctcggc agcattatcg
     3001 aggacacggc ggtgctggtg ctgatgacgc cgatcctggt gccgcttttg gacaatttcc
     3061 atatcgatcc ggttcatttc ggcatggtcc tgtgcatgaa cctgacgatt ggcctggtgg
     3121 cgccgccggt cgggcttgcc atgttcgtga cctgctcgat cggccagatc agcatcgagc
     3181 gcttcatgcg cgagtcgtgg ccgttcctgc tggccctgct gctgttgctg ctcgccctga
     3241 tcgtttttcc gtcgatctcg ctttggcttc cccatcagat ggtgcacgta tgagccgcag
     3301 ccccttgctc gaacagatcg gctggaacgt cgaccggctg gtgacgacgg acttcccttc
     3361 ctacggcgtg atcggcccgc tctaccgggg cgcggtggcg ctccagggcg gaccgctgtg
     3421 catgcgcgcg gcggaaatgc tgaaggaaaa cgtcgcgccc ggcgacacgg tgatcctgct
     3481 gaccgggctt atcctgcccg gccactaccc ctatggcgag acggacgggc cgctgggcgc
     3541 ggccgtgctg gcgcgcgcac ttgccatcgg cctgaaagcg cgggtcctcg tggttgtgga
     3601 gccggagttg accggcctca tgggcgcgtt gctgcgcgtg gcggaattgc ccttcgtcac
     3661 gctggcggcg ctggagaccg aaagcggcct gcaccggccc gccgccgccg ttgtcggcct
     3721 gtcgcgcgac agcgccacgg cccatgccga cgccagaagc tggatcggca gcctcaacgc
     3781 caaggccgtg gtcgccatcg agaaggccgg caccaacgcg caaggcgtct accacatggt
     3841 tggcggcgcg gatatctcgg ccacggtcgg aaaaggcgat gcggtgttct cggtcgccgc
     3901 cgagcttggc ctggtcagaa tcggcatcgg cgaccgcggc aacgaattgg gaaccgggcc
     3961 gatagcggag gtcacgcgcg cgctgctgcc tttcggcacc gattgcgggt gtccctgcca
     4021 cggcggcgtc gcggaccaga caccggccga tctggcggtg ccggccgtca tctccaactg
     4081 gggcgcttat ggcgtcgctg cctgtctggc ggcgcttcac gagcagcccg acctgttgca
     4141 ttcggcatcg ctggaacggc ggctgacgga aacggccatt ctcaatggcg gtgtcgaagg
     4201 catgtcgggc cgcgcaaagc tggcggtgga cggcatcggc gtcgatacga gcgccgcgat
     4261 ggccgaactg ctggtcgaag tctaccgggc gcaatcggtg cgcgacccgt cgcctttttc
     4321 gacgccgctt atcaagaaac aataatcggg atgaatggga gaagatcatg attacgcgca
     4381 gaaccatgct tgccggtact tgcctggccg gtacgtcctt ggccgggctg ctttccggcc
     4441 tgaaactggc acaggccgcc gagttcacct ttcgcgtcgc ctcggtgctg gaagcgcggt
     4501 cggacctcta tggcggcttc gaccatttcg ccaagcgcac ctcggaactg accaatggcc
     4561 gcgtcgaggt gaccacttac aagaatacgc ttggaaaggg cgagcgccag ctgttcgagg
     4621 cggtccaact gggtaccatc gagggcacgg tcgccaccac cggcacgctg agcgccttcg
     4681 tgccggaggc cgaccttttc aacctgccgt tcctgttcaa gaacaccgcg catgccttca
     4741 aggtggtgga cggcgaaatc ggcgacgagt tcaacaagaa gatgatcgcc aagggcttcc
     4801 gcatccttgg ctggtgggtg accggcacgc gcaatacctc gaacaatgtg cgcgcgatca
     4861 attcgccgga tgactacaag ggcctgaaga tccgcacgat ggaatcgcca cccttcatcg
     4921 agctttacaa gtcgatgggc gccataccgg tgccgatgtc gatctccgag gtctataccg
     4981 gcctcagcca gggcacgatc gacgggatcg acggcagcct gccggcgcag cttgagttca
     5041 agcatatcga ggtgctcaaa tacgccgccg tcaccgacca cgtagtcttg ttgtacgcct
     5101 tcgtcgtctc ggagcgctgg tggcagcaac tgccggagga cgtgcacgcc gcaatcatga
     5161 aagccgaagc ggaaggccgc gacatcgagc gcaaggacga cgaagcctac aacaagaagg
     5221 tcgaggccga atgggtgaag gaaggcaaga ccgtcaccca cccggatatc aagaagttcc
     5281 aggatat
//
LOCUS       DASYCS010000032         3217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3338903,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000032 DASYCS010000000
VERSION     DASYCS010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3338903"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1662)
                     /locus_tag="VGM46_01365"
     CDS             complement(139..1662)
                     /locus_tag="VGM46_01365"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEY4191269.1"
                     /translation="MNPTAQEMRAEPALEPGATILELRGLEKKYPGTHALKPVDLAFE
                     AGEIHAIVGENGAGKSTLIKLLTGVITRTSGEVFWEGRPVALATPYEAMALGINAVHQ
                     EMVLCRHLTVAANMFLGEEEVRYGLLQQRKMVRDAQKIIDDIGFDLPAHVGLGDLTIG
                     QQQLIAAARATVRGTRFLIFDEPTAYLTRKEADQLFTLIRRLKAEGVTIVYISHRMEE
                     VFELADRVSVLRDGTLVGTRKIAETDDAELVRMMINRSIEQIYHKETFTPGAAILEAK
                     NLSGKGFEEVSMTVREGEIVGLYGLIGAGRSEFVTTLYGRNRKSAGEILWQGRQVAVD
                     SEHDAIKLGMALAPESRRDQGLCLNLPVGLNLNLPIYKRLSQNMLISGSEEKSHADRQ
                     IAGLRIKTPTRNVLASSLSGGNQQKIVIGKWLNHGAKLFIFDEPTVGVDVGTKAEIYR
                     LFGQLLAGGAGIILISSYLPEVYELSDRLHVFRAGRLIASHGFRAASHEDVLTQAIG"
     gene            complement(1761..2756)
                     /locus_tag="VGM46_01370"
     CDS             complement(1761..2756)
                     /locus_tag="VGM46_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006200457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY4191270.1"
                     /translation="MEFINKRLNRRTFAALAAASILAGIAPAGPASAADVTIPIIVKD
                     TTSFYWQIVLAGARQAGKDLGVNVPELGAQAETDVNGQISILENAVAGNPAAIVISPT
                     EAKALGKPIDEAASKVKIIGIDSSADSKAFTSFLTTDNVQGGRVAADGLAAAVGAANG
                     GKMEGKVALITALPGAGSLEQRRQGFTEQLKAKYPGLELIADKYADGQATTGLNIATD
                     LITANPDLKGIFASNLIMAQGVGQAIAENNLAGKVALVGFDSDEKLIKFLTDGVISAL
                     VVQDPYRMGYDGIKTALAASKGEKVEEFVDTGANLVTKANMKDAKIDALLNPKLN"
BASE COUNT          560 a          953 c         1014 g          690 t
ORIGIN      
        1 agggcgatcc acaacgccgc cagaagcaaa agcagcgtca ggccgaacag cacgttgtag
       61 ttccgcttcg gcttttcgat ggatacggtt tcggtgctca tctttcgctc cccgttttta
      121 ttgttatccg cgcccgcgtc agccaatggc ctgcgtcagc acgtcttcgt ggctggctgc
      181 gcggaagccg tggctggcga tcagccggcc ggcgcggaac acatgcaacc tgtcggatag
      241 ttcatagact tccggcagat aggacgagat caggatgatg cccgccccgc ctgccagcaa
      301 ctggccgaac agtcggtaaa tctcggcctt ggtgccgaca tcgacgccga ccgtcggttc
      361 gtcgaagatg aacagcttgg cgccgtggtt cagccatttg ccgatgacga tcttctgctg
      421 gttgccgccc gacaggctgg atgccagcac gttgcgggtg ggcgtcttga tgcgcaggcc
      481 ggcgatctgg cggtcggcgt gggatttctc ctcgctgccg gaaatcagca tgttttgcga
      541 aagccgctta tagatcggca aattcaggtt gaggccgacc ggcaggttga ggcagaggcc
      601 ctggtcgcgg cggctttcgg gcgccagcgc catgccaagc ttgatggcgt cgtgctcgga
      661 atcgaccgcg acctgcctgc cttgccaaag gatttcgccc gccgacttgc ggttgcggcc
      721 atagagcgtg gtgacgaatt cgctgcggcc agccccgatc aagccataaa ggccgacgat
      781 ctcgccttcg cggacggtca tggaaacttc ttcgaagcct ttgcccgaca ggttcttcgc
      841 ttcgaggatg gcagcgcccg gcgtgaaggt ttccttgtgg taaatctgct cgatggagcg
      901 gttgatcatc atgcggacca gttcggcgtc gtcggtctcg gcgatcttcc tggtgccaac
      961 cagcgtgccg tctcgcagca cggaaacccg gtcggcaagc tcgaatactt cctccatgcg
     1021 gtggctgatg tagacgatgg tgacgccttc cgccttcagg cggcggatca gcgtaaacag
     1081 ctggtcggcc tccttgcggg tcagataggc cgtaggctcg tcgaagatca ggaacctggt
     1141 gccgcgcacg gtggcgcggg cggcggcgat caactgctgc tggccgatgg tgagatcgcc
     1201 aaggccgaca tgggcgggca ggtcgaagcc gatatcgtcg atgattttct gggcgtcgcg
     1261 caccatcttg cgctgttgaa gcaggccgta gcgcacctct tcctcgccaa ggaacatgtt
     1321 ggcggccacc gtcagatggc ggcagagcac catttcctga tgcacggcat tgatgccgag
     1381 cgccatcgcc tcgtaagggg tggccaacgc caccggccgg ccttcccaga acacttcgcc
     1441 ggaagtgcgg gtgatcacgc cggtcagaag ctttatgagg gtcgattttc cggcgccgtt
     1501 ctcgccgacg atagcgtgga tttcgcccgc ctcgaaggca aggtcgaccg gtttcagcgc
     1561 atgtgtgccg ggatatttct tttccaggcc tcgcagctcc agaatggtcg cgcccggttc
     1621 gagcgctggc tcggcacgca tttcctgcgc cgtcggattc atggcgatcc tcccgattgc
     1681 ctcgtcacat gccggccttc gctgctacgg caaaggatcc ggggccatga cagccccgga
     1741 aaagggtttg accggcgcga tcagttgagc ttcggattga gcagcgcgtc gatcttggcg
     1801 tccttcatgt tcgccttggt gacgaggttg gcgccggtat cgacgaattc ctcgaccttt
     1861 tcgcccttgg aggcggcgag cgccgtcttg atgccgtcat agcccatccg gtacgggtcc
     1921 tgcacgacca gcgccgaaat gacgccgtcg gtcaagaact tgatcagctt ctcgtcgctg
     1981 tcgaagccga cgagagccac cttgccggca aggttgtttt cggcaatcgc ctggccaacg
     2041 ccctgcgcca tgatcaggtt cgaggcgaaa atgcctttca agtcaggatt ggcggtgatc
     2101 aggtcggtgg cgatattgag gccggtcgtg gcctggccgt cggcatattt gtcggcgatc
     2161 agttccagcc ctggatattt cgccttgagc tgttcggtaa agccctgtcg gcgctgttcc
     2221 agcgagccgg caccgggaag cgcggtgatc aaggccacct taccctccat cttgccgcca
     2281 ttggccgcgc caaccgccgc cgccaggcca tccgccgcca cgcggccgcc ctgcacgttg
     2341 tcggtggtga ggaaggaggt gaaggccttg gaatcggcgc tggagtcgat gccgatgatc
     2401 ttgaccttgc tggccgcttc gtcgatcggc ttgccgagcg ccttggcttc ggtaggcgaa
     2461 atgacgatgg cggcgggatt gccggcgacg gcattttcca gaatggaaat ctggccgttg
     2521 acgtctgttt cggcctgtgc gcccagttcg ggcacgttga cgccgagatc cttgccggcc
     2581 tggcgggcgc cggccagcac gatctgccaa tagaaggaag tggtgtcctt gacgatgatc
     2641 gggatggtta catcggcggc cgaagccggt ccggccggcg cgatgccggc cagtatcgaa
     2701 gcggcggcga gtgccgcgaa ggtgcggcga ttgagacgtt tattgatgaa ttccataggt
     2761 ttcctcccta aggcgcctgg tttatctccc tcaaatccgg tttgaacagg cgtggcccaa
     2821 acagaacttt atcgataaaa aacattattt ccaataatgc attccagcaa gggtgtcgag
     2881 agactttaca gttcgcgccg cccgtttgcg gcaatcggtt cctcccgtgt aatgaaactt
     2941 aaattccaat tgcagtcaat gcagaataat tgttccattt accgccgatt tcggccctgc
     3001 aattcgaccg gtgacggatc gaccggggac cgcctggcgg atgccggttc attcgcgcac
     3061 cgctccggtc aatttgccct gcacggtcgt gtgccggcgg tatttttcat attcaccggc
     3121 cggaacgcgg gtggtgagca gatccatatt gcgcttcacg ctggaagcct tggccgtgcc
     3181 gataagcacg gtggccacgc atggttcgtc gagcggg
//
LOCUS       DASYCS010000033         6124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4063627,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000033 DASYCS010000000
VERSION     DASYCS010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 6124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6124
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4063627"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="VGM46_01375"
     CDS             <1..739
                     /locus_tag="VGM46_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008875777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEY4191271.1"
                     /translation="NRLIDLSERAAEMLNYANVGTAEVKVDYIGRAPLEGNDDQYLMA
                     SYHPGNRAPDPSDGLPAGVMIALNGATPSASGSASSIPFPGQLTNSAATVETPTVTAS
                     ASGFAQLPEFGPIVTERPDIASQPPVPFSLASLSYADERVQRAWGAFAALDETGSSAW
                     KQATSADGSKGASSYIAVGTYASAVEAKAMASTLTGFGKSEIQQSNRDGKPWYSVNLY
                     PDGHGNLDDLLQAAWSRGAPDALAVRD"
     gene            915..2018
                     /locus_tag="VGM46_01380"
     CDS             915..2018
                     /locus_tag="VGM46_01380"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009449471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEY4191272.1"
                     /translation="MNEASAQLFETKAAQAYMVDAETGTVLFSKDADKIIQPASLAKL
                     MTVEMAFNAIKSGRLKLDDTFVVSENAWRTGGAPSGTSTMFAKIKSTIRVEDLIQGVA
                     VQAANDGCIVIAEGMAGSEDNFALQMTERARKIGFDNSIFVNSTGLPAAGQQTTVKDL
                     ALLALHLSRDYPDLYRYFGGKDFTWNKISQRNRNPLLGMDIGADGLSVGNSEQSGYGI
                     VGSVSQGGKRVVAAMSGLASDRERSEEARKLLEWGLRSFEKTEIFSRDEGVGEAEVFG
                     GVKSTVALKANGPVDIFLPITNRDKMTARIVYDGPLVAPVEKGQPVGSLRVWIGDTLS
                     QETPLFAAETVNVGSLPQRALDATKELAIGWLR"
     gene            2087..2779
                     /gene="tmk"
                     /locus_tag="VGM46_01385"
     CDS             2087..2779
                     /gene="tmk"
                     /locus_tag="VGM46_01385"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015317900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEY4191273.1"
                     /translation="MAHGVFITFEGGEGAGKSTQIDRLAGKLRAKKYDVVVTREPGGS
                     PGAEAVRHVLLSGAAEPFGPEMEALLFAAARSDHVEQLIKPAIARGAIVLCDRFMDSS
                     RVYQGVTGGVDAGLMDALENVAVHGAVPDMTLILDIDPAEGLARAASRRGEHAVADRF
                     EKEVLEIHRQRRAAFLAIAAAEPDRCIVIDAAALPEVVEDSITAAVFAALEVRIPARS
                     NRVTHSNRAAKA"
     gene            2776..3831
                     /locus_tag="VGM46_01390"
     CDS             2776..3831
                     /locus_tag="VGM46_01390"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEY4191274.1"
                     /translation="MSFQRIAPEQHDTLDGVPEPSETPRLIGHAQAASMLVAAYRAGK
                     LPHALIVAGPVGIGKATFGFHLAHHLLKYPQYETAPAMLAVPDPTSSLFRQIATGAHP
                     SVLHLTRPLNEKTKAYKSVVTVDEIRKVSRFLSMTSHDGGYRIVIIDPADDMNTNAAN
                     ALLKNLEEPPARTLFILVVHAPGSLLPTIRSRCQLVRLSPLGVDDLMSVLDAADQAPP
                     TGAAERAALASRAGGSARMAILLAHYGGLEIAETLDAMIAANKPDIVGAHRIADAVSG
                     RDRAIAFDIFNRRVLDLLSDAASGAAQSGALERAKALSDTWHEAVNAISDTETYNLDK
                     KQHALMMIDRLNAAIRM"
     gene            3925..5475
                     /gene="metG"
                     /locus_tag="VGM46_01395"
     CDS             3925..5475
                     /gene="metG"
                     /locus_tag="VGM46_01395"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEY4191275.1"
                     /translation="MSREKFYITTAISYPNGKPHIGHAYELIATDALARFQRLDGKDV
                     FFLTGTDEHGIKMLQTAKREGISPRELADRNSAEFRRMATALGASNDDFIRTTEERHY
                     RASQAIWKAMAANGDVYKGGYAGWYSVRDEAYYGEDETEVRADDVRYGPQGTPVEWVE
                     EESYFFKLSAYQDRLLALYESQPGFIGPSERRNEVMSFVKSGLKDLSISRTTFDWGVP
                     VPGDEKHVMYVWVDALTNYITGIGYPDENDEKWGFWPADAHIIGKDIVRFHAVYWPAF
                     LMSAGIELPKRVFGHGFLFNRGEKMSKSLGNVIDPFTMVEHYGLDQVRYFFLREVPFG
                     QDGSYSHEAIVNRTNADLANDLGNLAQRSLSMIAKNCDGLVPVCGELTDADKALLDQA
                     SAALVTARKAMAEQAIHLALAAIFAVVAEANRYFAAQAPWALKKTDAARMETVLWTTA
                     ETIRRVAVMCQPFMPTSAAKLLDVLAVPSENRNLADVDRAGGLVAGTALPAPQPVFPR
                     YVEQAASE"
     gene            5481..>6124
                     /locus_tag="VGM46_01400"
     CDS             5481..>6124
                     /locus_tag="VGM46_01400"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006333564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEY4191276.1"
                     /translation="MLVDSHCHLDFPDFAQERAAIVARAVAAGVGRMVTISTRVKKFP
                     QVIEIAEQFEEVYCSVGTHPHNADEELDITADDLVRLAAHPKVVAIGEAGLDYHYDKA
                     PRQAQAQGFRNHIAAARITGLPLVIHARSADEDMAAVLEEETGKGAFPFILHCFSSGR
                     GLAETGVALGGYVSFSGILTFKNSAELRAIAADIPRDRLLVETDAPYLAPTPFRG"
BASE COUNT         1193 a         1868 c         1890 g         1173 t
ORIGIN      
        1 caaccggctt atcgacctgt cggaacgtgc cgctgaaatg ctgaactacg ccaatgtcgg
       61 aaccgccgag gtcaaggtgg actatattgg acgcgccccg cttgaaggca atgacgacca
      121 gtatttgatg gcctcatacc atcccggcaa ccgcgctccc gatccttccg atggcttgcc
      181 ggcaggcgtg atgattgccc tgaacggcgc gacgccaagc gcatccggca gcgcctcgtc
      241 gatccctttt ccaggtcagc tgaccaattc ggcggcaact gttgaaaccc cgacggtaac
      301 ggcatccgcg tccggctttg ctcaattgcc tgaattcggg ccgatcgtga cggagcgtcc
      361 cgatattgca tcgcagccgc cggtgccgtt ctcgctggcc tcgctatctt atgccgacga
      421 gcgcgtgcag cgtgcgtggg gcgcatttgc cgccctggat gagacaggca gttccgcatg
      481 gaagcaggcg accagcgccg atggttcaaa gggcgcctcc agctatatag ccgttggaac
      541 ctacgcaagc gccgtcgagg ccaaagccat ggcttcgacc ctgaccggct tcggcaaaag
      601 cgaaatccag caatcgaacc gcgacggtaa gccgtggtac tcggtcaacc tttatccaga
      661 tggtcacggc aaccttgacg atctgttgca ggcggcgtgg tcgcgtggcg cgcccgacgc
      721 gcttgccgtg cgcgattgac ctttcggacg gacgccggcc acccatcggt tttccggatt
      781 gatcctgtag gcaagcctgc tagccttgtt gcgaccggct gtgggagctg gcgccaacag
      841 ggcatttttg gatgaaaagc tggattttcc agatagttgc ggggtttctc gggcttatat
      901 tctcagggct gccgatgaac gaggccagcg cgcaactgtt cgagaccaag gcggcgcagg
      961 cctatatggt cgacgccgaa accggcacag tcctgttttc caaggatgcg gataaaatca
     1021 ttcagccggc ttcgcttgcc aagctgatga ccgtggaaat ggcattcaac gcgatcaaat
     1081 ccgggcgact gaaactcgat gacaccttcg tcgtcagcga aaacgcgtgg cgcaccggcg
     1141 gcgctccctc cggcacttcg acgatgtttg cgaagatcaa gtcgaccata cgcgtggaag
     1201 acctgatcca gggcgtggcg gtacaggccg caaatgatgg ctgcatcgtc attgccgagg
     1261 gcatggccgg ctcggaggac aattttgcgc tgcaaatgac cgagcgcgcc cgcaagatcg
     1321 gtttcgacaa ttcgattttc gtcaactcga ccggcttgcc cgcagccggc caacagacga
     1381 cggtcaagga tctcgctctg ctggccttgc acctgtcacg ggattaccca gatctctacc
     1441 gctatttcgg cgggaaggat tttacctgga acaagatatc ccagagaaac cgtaacccgc
     1501 tgctgggaat ggatatcggc gcggatgggc tgagcgtcgg caatagcgag cagtcgggct
     1561 acggcatcgt cgggtcagtc agccagggcg gcaagcgcgt ggtcgccgcc atgagcggtc
     1621 ttgccagcga cagggagcgt tcggaagagg cgcgcaagct gctggaatgg ggcctgcgtt
     1681 cttttgaaaa gacggaaatt ttctccaggg acgaaggcgt cggcgaggcc gaggtgttcg
     1741 gcggagtgaa gtctacggtg gccctgaaag ccaacggacc tgtggatatt tttctgccca
     1801 tcaccaaccg cgacaagatg acggcgcgca tcgtctatga tggaccgctg gtcgcgccgg
     1861 tggaaaaggg gcagccggtc ggctcgttac gtgtctggat aggcgatacg cttagccagg
     1921 aaacgccgct gttcgcggct gaaaccgtca atgtcggttc gctgccgcaa cgcgcgctcg
     1981 atgccacgaa ggaattggcg atcggctggc ttcgataaga taaccggcgt ggacctttag
     2041 agccgcccgg actatgacca tccccgcgag aaaaaaggtc tgtcgcgtgg cacacggtgt
     2101 tttcatcacg ttcgaaggcg gagaaggggc cggcaagtcg acccagatcg accggctggc
     2161 aggcaagctg cgcgccaaga aatacgatgt cgtcgtcacg cgtgagccgg gtggttcacc
     2221 gggcgccgaa gccgtccgcc acgttctttt gtccggcgcc gccgagccgt tcggtccgga
     2281 aatggaagct ctgctgttcg cggcagcccg ttcggatcat gtcgaacagc tcatcaagcc
     2341 tgcgatagcg cgcggggcca ttgtgctgtg cgaccgcttt atggattcct cgcgcgtcta
     2401 ccagggcgtt accggcgggg tcgatgccgg tttgatggac gcgttggaaa atgtcgccgt
     2461 gcacggcgcc gttcccgaca tgacgctcat cctcgacatc gacccggcag agggtctggc
     2521 gcgcgcggca tcgaggcggg gcgaacatgc cgtggcagac cggtttgaaa aggaagtgct
     2581 ggaaatacac cggcagcggc gcgcggcctt tctggcgatc gcggcggcgg aaccggatcg
     2641 ctgtatcgtc atcgacgcgg cagctttgcc cgaggtggtc gaggacagca tcacggcagc
     2701 cgtgtttgcc gcgctggagg tccgcatacc agcccgttcc aatcgggtca ctcactccaa
     2761 ccgggctgcc aaggcatgag cttccagcgg attgctcccg aacagcacga caccctggac
     2821 ggcgttcccg aaccttcgga gacgccacgg ctgatcggtc atgcgcaggc tgcatccatg
     2881 ctggtcgcgg cctatcgggc cggcaagctg ccgcatgcgc tgatcgttgc cggcccggtt
     2941 ggcatcggca aggcgacctt tggctttcat ctcgcccatc acctgttgaa atatccgcaa
     3001 tatgagaccg cgcctgccat gcttgcggtt cccgacccga cctcgtcgct gtttcgccag
     3061 atcgccaccg gggcccaccc ttcggtgttg cacctgacgc ggccgctgaa cgaaaagacc
     3121 aaggcctaca agagcgtcgt cacggtcgat gagattcgca aggtcagccg tttcctgtcg
     3181 atgacctcgc acgatggcgg ttaccggatc gtcatcatcg atccggcgga cgacatgaac
     3241 accaatgccg ccaatgcgtt actgaagaac cttgaggaac cgcccgcgcg caccctgttc
     3301 atcctagtgg tacacgcgcc gggcagcctt ctgccgacca tccgttcgcg ctgccagctc
     3361 gtgcgcctct cgccgctcgg cgtcgacgat ctgatgagtg tcctcgatgc tgccgatcag
     3421 gcgccgccca ccggcgccgc cgaaagggcg gccttggcca gccgggcagg cggcagcgcg
     3481 cgcatggcga tcctgcttgc ccattatggt ggccttgaaa tagccgaaac cctcgacgcg
     3541 atgatcgcag cgaataagcc cgatatcgtc ggcgcgcacc gcatcgccga cgccgtttcg
     3601 ggccgggaca gggcgattgc cttcgacata ttcaaccgcc gcgtcctcga ccttttgtcc
     3661 gatgcggcga gcggtgctgc gcaatcgggg gcgctggagc gcgccaaagc cctttccgac
     3721 acttggcatg aagccgtgaa cgcgatatcc gataccgaga cctacaatct cgacaagaag
     3781 caacacgccc tgatgatgat cgaccgcttg aacgcagcga ttagaatgtg accgcgccgg
     3841 agcatcgggt ggcgttcgcc aggccgatgc gctatgccac cgcgacctca tttcatccgg
     3901 cattcaaggc aagacggttc gatcatgtca cgcgagaaat tctacataac caccgcgatt
     3961 tcctacccaa acggcaagcc gcatatcggc catgcctacg agttgattgc gaccgacgct
     4021 ctcgcccgct tccagcgcct tgacggcaag gacgtattct tcctgaccgg cacggacgag
     4081 cacggcatca agatgttgca gacggcgaag cgggaaggca tttcgcctcg cgaattggcg
     4141 gaccgcaatt ccgcggaatt caggcggatg gccaccgccc ttggcgcctc caacgacgat
     4201 ttcatccgca cgaccgaaga gcgccactac cgcgcctcgc aggcgatctg gaaggcgatg
     4261 gcggccaatg gcgacgtcta caagggcggg tatgccggct ggtattcagt gcgcgacgaa
     4321 gcctattacg gcgaagacga gaccgaagtg cgggccgatg acgttcgcta cggcccgcag
     4381 ggaacgccgg tcgaatgggt cgaggaagaa agctacttct tcaagctttc ggcctatcag
     4441 gaccggctgc tggcacttta cgaaagccag ccgggcttca tcggcccatc ggagcggcgc
     4501 aacgaggtga tgagcttcgt taaatccggg ctgaaggatc tgtccatttc acgcaccact
     4561 ttcgactggg gcgtgccggt gcccggcgat gaaaagcacg tcatgtatgt ctgggtcgat
     4621 gcgctgacga attacattac cggcatcggc tatcccgatg aaaatgatga gaaatggggt
     4681 ttttggccgg ctgatgccca catcattggc aaggatatcg tccgcttcca cgcggtctat
     4741 tggccggcat ttctgatgtc ggccggcatc gaattgccca agcgtgtatt cggccatggc
     4801 tttttgttca accgcggcga gaagatgtcg aaatcgctgg gcaatgtcat cgatccgttc
     4861 acgatggtcg agcattacgg gctggatcag gtccgttatt tcttcctgcg cgaagtgccg
     4921 tttggccagg acggcagcta tagccatgaa gccatcgtca accgcacgaa tgccgacctt
     4981 gccaacgacc tcggcaatct ggcccagcgg tcgctgtcga tgatcgccaa gaattgcgac
     5041 ggcctcgtgc ctgtgtgcgg cgaactcacc gatgccgaca aggcgcttct cgatcaggca
     5101 tcggcggccc ttgtaacggc gcgcaaggca atggcggagc aggccattca tctagcgctg
     5161 gccgcgatct tcgccgtcgt tgccgaggca aaccgttatt tcgccgcgca ggcgccatgg
     5221 gcgctgaaga agacggatgc cgcccggatg gaaaccgtgc tgtggaccac ggcggagacg
     5281 atccgccgcg tggcggtcat gtgtcagcct ttcatgccga catcggccgc aaagctgctc
     5341 gacgtgctgg ccgtgccttc tgaaaaccgc aacctcgccg acgtcgatag agcgggtggg
     5401 ctggtggcgg gcaccgcctt gccagcgccg cagccggtct ttccgcgtta tgtcgagcag
     5461 gcagcatcgg agtagctgcg atgctggtcg acagccattg ccatctcgat ttccccgact
     5521 tcgcgcagga gcgcgcagcg attgtcgccc gcgcggttgc cgcgggcgta ggccgcatgg
     5581 tgacgatctc cacgcgcgta aagaaatttc cgcaagtaat tgagattgct gaacagtttg
     5641 aagaagtcta ctgctcggtc ggcacccatc cgcacaatgc cgatgaggaa ctcgacataa
     5701 cggccgatga tttggttcgc ctggcggcgc atccaaaggt cgtagccatc ggcgaagccg
     5761 ggctggacta ccactacgac aaggccccgc gccaggcgca ggcgcaaggc tttcgcaatc
     5821 atatcgcagc ggcgcgcata accggcctgc cgctggtgat ccacgcacgc agcgccgatg
     5881 aagacatggc cgcggtcctt gaggaagaaa cagggaaggg cgcatttccc ttcattttgc
     5941 attgcttttc gtcgggtcgc ggactggctg aaacgggcgt tgcgcttggc ggttatgttt
     6001 ccttttccgg catcctgact ttcaagaatt cagccgagct gcgcgccatc gcggcagaca
     6061 ttccgcgcga ccgcctgctt gtcgaaaccg acgcgccata tctcgcgccg acgccatttc
     6121 gtgg
//
LOCUS       DASYCS010000034        19693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3484604,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000034 DASYCS010000000
VERSION     DASYCS010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 19693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19693
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3484604"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="VGM46_01405"
     CDS             <1..808
                     /locus_tag="VGM46_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY4191277.1"
                     /translation="IVNTVIAGWHGRKKRGIGENFWQFRPYVEGDSSRIDWRRSARDD
                     HTYVRDREWEAAHTVWLWADPSPSMLYKSTAASVSKQSRALVLALAMAELLSRGGERI
                     GWPGLTDPFTARNGAERIAAHLSHAADLSAKPDLSGIRRFSDAVLISDFLDPVEETFQ
                     WLDELARRGVRAHLVEVADPAEESFPYAGRTEFTDPETGERLTAGRAEMLGDDYRRLY
                     AARRAELASRCQRLGWSFTTNHTDRLASDALARLHLAMGGDSLHAGKIGA"
     gene            805..3630
                     /locus_tag="VGM46_01410"
     CDS             805..3630
                     /locus_tag="VGM46_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006338569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HEY4191278.1"
                     /translation="MSWLPLSFGAPMVLWGLLALPVIWWLLKLTPPKPQAEVFPPFKI
                     LARVLKRQETPQRSPWWLTLLRLLMAALVIAALAEPVFNPRETLPTQGSALALVIDND
                     WAAAPDWDKRVATAERLISDAGSKSMPVVVALTAEKPNAEIGPFDAAAALDRLRAAKP
                     RPVPTDRPAIYARIAETLATLPGASVAILSDGLAAKGDETAFKTLLSKNAANVVWAEP
                     ERVAAIALTSAENEVDGFALTAIRAPGEQTAAKVTAGAFDDKGRRIADATLSFAPGET
                     KGTGTMAVPFELRNDFSQIAIDGERHAGAVRVLDDSAKRRRVGLLSQAEADQAQPLLS
                     PLYYIRRALSPFADLVEPSSADLADAIPQLLDRKPAMIVMADVGAIPQQVRQRLVDWV
                     NNGGTLVRFAGSRLAAAGNDDDLLPVRLRTGERSLGGTLSWTTPQPVAEFPKTGPFAD
                     LAAPKEVTVSRQVLAEPGPDIADRTWASLADGTPLVTGLKKGKGTLVLFHTTPEATWS
                     NLPISGSFVEMLRRIVQLSRNQGAVASNTEITATALAPYRMIAADGALVPPTPDARPL
                     VPNAKAPVTLENPPGLYGSDAGVYAHNLLDADSTLTPLARPNISVPVTAMRYAFDESR
                     HLKGSLVLAALALMVLDTLAVFWMGGLFSRRSSARRLSSATTAAVALVALAALSAYPG
                     SARADDSKPGDAQAIEAVAKTRIAYVLTGEPGVDAISRAGIEGLDRFLIEKTALEPGE
                     PAGVDIASDELSFYPLIYWPIDAAAAMPSEAAIARIDAYMQQGGTVLFDTRDQFATGI
                     GADSASPATTRLRDILGNLNVPPLEPVPADHVLTKSFYILPEFPGRFNGSPLWVEASL
                     DAGNADNRPVRTGDGVSPIMITANDFAGAWAIDANGDPLLPTVPSDPLQRIYALRAGV
                     NIMMYMLTGNYKSDQVHVPILLERLGQ"
     gene            3632..5704
                     /locus_tag="VGM46_01415"
     CDS             3632..5704
                     /locus_tag="VGM46_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191279.1"
                     /translation="MNWSLAFEPLFSWPWLAAALAPLAILAIIGLWFRQRGGWLRALA
                     LAALAAALLNPVFLDEDREPLKSVVAVVVDRSQSQDIGERTAQTDAALAGVKERLARF
                     KQFDVRVVETGRASAAEDRTETRLFSALDSAFRDVPPSRIGGAVMITDGEVHDVPKGA
                     PEFAAPLHALITGNDHEKDRRIRFERAPRFGLVGKPLDMSFRVIASDNQSGPVDVRVS
                     VNGQQVAVEHAFIGQEMPLQVTIPNAGRNIIELAIPKQEGELTEINNRAIALVDGIRE
                     NLRVLLVSGEPHAGERTWRNLLKSDASVDLVHFTILRPPEKQDGTPINELSLIAFPTR
                     ELFVEKINQFDLIIFDRYQHRDVLPILYYDYIAEYVEQGGALLIAAGPEYAGEQTIAR
                     TPLMAALPAMPTGEVIDKAFYPRLTDIGQRHPVTRGLDGSGVEPPHWGRWFRTIGVEK
                     PEGEVVMKGADNRPLLLLDHKGEGRVGMLLSDQGWLWARGYEGGGPHVELYRRIAHWL
                     MKEPELEEERLTADGRGMVLEVRRQTMSDAPGAAQIITPSGKALTVKLQKSEPGVFTG
                     SVEVKEIGLYQVGNGNLTALAHVGPVNAPEFTDVVSTENKLKPATDATGGSVRRLAAS
                     SGNGVVLPSIVPVRSSGEAAGNDWIGLRATDDSVLKAVSRVPLFGGFLGLGVLLLAMG
                     SMWYREGR"
     gene            5781..6254
                     /locus_tag="VGM46_01420"
     CDS             5781..6254
                     /locus_tag="VGM46_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018496342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY4191280.1"
                     /translation="MAEDNDPQRAWDLIEKIGFCMLATNDGRDIRSRPMTAHAEREEN
                     AVYFLLDLKDHKNLDAFASSCLAFADSGDMNYVSVTGSGEVSNDRAKIGELWSSPYKA
                     FWDGPDDPNIRVLKITPKDAQYWDGPGKIASYAKMLAAAVSDAKPDLGDHGKVRM"
     gene            complement(6357..6629)
                     /locus_tag="VGM46_01425"
     CDS             complement(6357..6629)
                     /locus_tag="VGM46_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191281.1"
                     /translation="MKKSFVSALGLAIALAFSAPMLGAGSASAASMHTPSSHRKVHHH
                     HTVVHHHKHHVSHKMHHASGHMTHHRAHKKHAVHHSAHKMHVAKKY"
     gene            complement(6866..7183)
                     /gene="clpS"
                     /locus_tag="VGM46_01430"
     CDS             complement(6866..7183)
                     /gene="clpS"
                     /locus_tag="VGM46_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEY4191282.1"
                     /translation="MSPPTNDPLTIARVKPQPKTERPKLYKVILVNDDFTPREFVVTV
                     LKGEFKLSEDQAHRVMITAHRRGVCVVAVFTKDVAETKAQRATDAGKAKGYPLMFTAE
                     PEE"
     gene            complement(7301..7531)
                     /locus_tag="VGM46_01435"
     CDS             complement(7301..7531)
                     /locus_tag="VGM46_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191283.1"
                     /translation="MNKSLLSALGVAVALAFSVPAMSAANAAATTTAAAPAAATTAPA
                     VKKPVHKVAQKKVVKKSAHKKVAPKKAAKKAM"
     gene            complement(7569..7883)
                     /locus_tag="VGM46_01440"
     CDS             complement(7569..7883)
                     /locus_tag="VGM46_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006338574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191284.1"
                     /translation="MQRYPTRLVSALLPVLLITATVGQSDAKVASSKVLVTRSERCSR
                     LSRQVDEAMETHAKAVQVAEAKALQRKANRFCADRKQAQGIRILAQALKVLGVSPADP
                     NQ"
     gene            8107..8820
                     /locus_tag="VGM46_01445"
     CDS             8107..8820
                     /locus_tag="VGM46_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY4191285.1"
                     /translation="MKSDPHILIVDDDKDIRDLLQEFFRKRGLHASVAADGVEMESVM
                     RRAQIDLIVLDVMLPGKSGLELCRELRARYPTPIIMLTAVTETTDRVVGLEMGADDYV
                     PKPFDPRELLARVRAVLRRNDPETRKEPQRQVYRFAGWSMDCSRRRLMAPGDVLVELT
                     GAEFNLLHTFVRSAQRVLTRDQLIELSGGEGGASYDRSIDILVSRLRRKMEDDPRTPK
                     LIMTVRSGGYQFMPETVAE"
     gene            8817..10229
                     /locus_tag="VGM46_01450"
     CDS             8817..10229
                     /locus_tag="VGM46_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY4191286.1"
                     /translation="MRRFLPQTLPAWVLLVVITGLLVSQLTTFYLVQRDRAASNDVVE
                     LYRLNERTFSYVQLMHKASPPDRKQLAAGLSNSGNALTVSDTPVVSSPIATDDSLAEL
                     EDILVGRLSKFGVIDARVRRDAAERDATGLGQTDTDTELGQVERDLLALATNFAKSKK
                     LTASVEFADGQWLNFTTPITPVAPVLNLDSTPLSAIVAALVVIMSIWSLRRLTAPYRT
                     LERAVGKISADLRSPPLDENGSREYRSAARALNAMQLRLREYVEDREQLAAALAHDLR
                     TPLTRMRLRLELLGRSKARTALAHDLSDIDQIARSVIDFATFEMAEEKPERIDLASLA
                     SSIADSYDTVSFEREAERGMVCAARPIALRRCITNLVNNAVLYGKRVHLDLHRNGGNI
                     ALTIRDEGPGIAPEQFEQVFRPFVRIETSRSRETGGSGLGLTIARNIARSMGGDVRLS
                     NHPLGGLCAELTLPAITVTA"
     gene            complement(10391..11572)
                     /gene="choV"
                     /locus_tag="VGM46_01455"
     CDS             complement(10391..11572)
                     /gene="choV"
                     /locus_tag="VGM46_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015220 - choline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015871 - choline transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline ABC transporter ATP-binding protein"
                     /protein_id="HEY4191287.1"
                     /translation="MNVAVDFKNVDIVFGTEKPRALAMIDTGATRQEILAQTGAVLGC
                     ANCNLTVQEGDISVLMGLSGSGKSTLLRAVNLLNVPARGHVFVKQGDSAVDVVNCSAT
                     TLRAIRQKQVAMVFQQFGLLPWRTVEENVGFGLELAGVPEAERKERVHKQLKLVNLEQ
                     WAKKYAHELSGGMQQRVGLARAFATEAPILLMDEPFSALDPLIRTKLQDELLQLQAEL
                     KKTIIFVSHDLEEALKIGTHISIMEGGRIVQTGAPEDIVLRPANDYVREFIANVNPLS
                     VLTAWNVMRDRRELEESGDGWVWLDRRATTRFKVDGKGLVSAAERNGKPAIWVSCADV
                     EGQPEEASQVFWATPGTSLKTVMLAMHRSQTAPVALFDEQSRFVGSIGIRDVLSAVLR
                     R"
     gene            complement(11569..12423)
                     /gene="choW"
                     /locus_tag="VGM46_01460"
     CDS             complement(11569..12423)
                     /gene="choW"
                     /locus_tag="VGM46_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline ABC transporter permease subunit"
                     /protein_id="HEY4191288.1"
                     /translation="MDPVSKFLASYKIPIGSWGKDFFGFLTDNFDTIFRAFSSGLNLL
                     LDNLVDLLLAAPPVLLALVIAGLAWLLQRSRPLAVWVFIGLVFIINQGLWKQTVETMV
                     LVVAAAAVSMAIGVPLGIWAAHKPKVYRAMLPVLDMMQTLPTFVYLIPVLTLFGLGNA
                     PGLVVTIIFVIPTPVRLTYLGVTSVPKSIVEAGQAFGATKRQLLWKVELPAALPTIMA
                     GLTQAIMLSLSMVVFAALIGAGGLGTEINRALGSRRIDLGLESGLAIVVLAIILDRMS
                     RVTTGGRK"
     gene            complement(12662..13597)
                     /locus_tag="VGM46_01465"
     CDS             complement(12662..13597)
                     /locus_tag="VGM46_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006338588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY4191289.1"
                     /translation="MSRMNSFVAGLGLAAFLSTSVAFAADPESCKTVRLSDVGWTDIQ
                     ATTGVASVLLTALGYEPKVIQLSVPVTYASLKNKDLDVFLGNWMPSMTNDIKDYAADG
                     SVETISENLTGAGYGLVVPTYVADAGVKTLTDLGKFKDKFNGKIYGIEAGNDGNRIIL
                     DMIKNPADNLDGFELVESSEAGMLTQAEQSMKNNEWIAFLGWTPHPVMGAMKITYLDG
                     MGDSGFGAATVSTNVRKGYTTECPNAGTFIKNLKFNLDMEGQMMDAILKGGDANTVAT
                     DWLKKNPDAVTPWLAGVTTIDGGDAAAAVKTALGS"
     gene            13811..14422
                     /locus_tag="VGM46_01470"
     CDS             13811..14422
                     /locus_tag="VGM46_01470"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008877726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEY4191290.1"
                     /translation="MAKLYFHYATMNAGKTTMLLQASYNYRERGMETMLFIAGHYRKG
                     DSGCISSRIGLEAEAEMFRDGDDLFSRIAEHRSRTAVHCVFVDEAQFLDEEQVWQLAR
                     VADRLGIPVMCYGLRTDFQGKLFSGSRALLAIADELREVRTICRCGRKATMVVRLGED
                     GKVAKLGEQVAIGKDIYVSLCRRHWEEEMGRAVPDDFIGFTHG"
     gene            14582..14848
                     /gene="rpsU"
                     /locus_tag="VGM46_01475"
     CDS             14582..14848
                     /gene="rpsU"
                     /locus_tag="VGM46_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEY4191291.1"
                     /translation="MQVLVRDNNIDQALRVLKKKLQREGVFREMKARRAYEKPSEKRA
                     REKAEAVRRTRKAARKLAIREGLIAAPVKKLPLRPDQRTARPAA"
     gene            15044..16183
                     /locus_tag="VGM46_01480"
     CDS             15044..16183
                     /locus_tag="VGM46_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2333 family protein"
                     /protein_id="HEY4191292.1"
                     /translation="MLDPIVNFVTRIFGLLGRAIGFLVGIVLWPFLWLGRWYAQRGWM
                     LKAALGAVLLILIGLYANFFYATQWWNNFNPDYPAAMRQQKSVSVAGGPVAGNTGAAA
                     TGSASQAPASCQPSAIAGISAELIDFNVNQNSWVSSMVVSKMGFFGIDWRRTPFFDNK
                     AAFQLGINQVMRRTTTELVDTLGRVRGTSQIDQNLQDARTALAWNEDAWYLGWRGPTR
                     PTPSVYREAIGKLRAYNTSLEKCQVTFDARADNLMQFLDRIAGDIGSTSDILRSRIDA
                     SDAGWFDLRADDRFWFAYGQLYAYYGVLVAARSDFAAVIAERNLNTLWARMEEQSRAA
                     LNVQPWIISNGSESSAFFPSHLATMGFNMLQVRSNIVEIRSVLDR"
     gene            16273..16737
                     /locus_tag="VGM46_01485"
     CDS             16273..16737
                     /locus_tag="VGM46_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018429083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HEY4191293.1"
                     /translation="MTIYLLALLIGVIAGLRAFTAPAAIAWGAGLGWLSVAGTWAGFM
                     GHWIAAAVFTILAVIELVSDQLPSTPSRKVATQFGARIVVGAFSGAVIGATAGLTVGG
                     MTVGAIGAVIGTLGGAEVRARLAAAFGKDAPAAFIEDAVAIVVGLLIVHAAS"
     gene            16734..18110
                     /locus_tag="VGM46_01490"
     CDS             16734..18110
                     /locus_tag="VGM46_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEY4191294.1"
                     /translation="MTARKFDAIIVGAGQAGPPLAGRLTAAGMTVALVERKLVGGTCV
                     NTGCMPTKAMVASAYAAHVAGRAAHYGVTISGAVGIDMKKVKAVKDKVSNDARTGVEE
                     WIAGMKGCTLFRGHARFESANQISVGDDVLSAEKIFLNTGGRATVPDLAGIDTVDYLT
                     NSSMMDLDVLPRHLVVVGGSYISLEFAQIFRRFGSEVTIIEKGERLIGREDEDVSAAI
                     KSILENEGVKIHLGADTVSFAKKGNDTAVTFRAGQPPAIGSHVLLALGRTPNTQDLGL
                     DKAGIDQDKRGFIVVDDYLRTSVPGIWAMGECNGKGTFTHTAYNDYEIVAANLLDDDP
                     RKVSDRIEAYGLFIDPPLGRCGMTEAAARKSGRKVLVGQRPMTRVSRAVEKGETQGFM
                     KILVDADTDEILGCCVLGPGGDEAVHGVLDLMYAKAPVTTLRRAVHIHPTVAELLPTI
                     AQELKPLG"
     gene            18230..19417
                     /locus_tag="VGM46_01495"
     CDS             18230..19417
                     /locus_tag="VGM46_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006338616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4191295.1"
                     /translation="MPLPILALAIASFCIGTTEFVIMGLLPEVAVDLKVSIPSAGLLV
                     TGYALGVVFGAPVIAVATAHMRRKPVLVALASLFVVGNLLCAIAPTYWTLMAARVITA
                     FGHGAFFGIGSVVAASLVPRNKQASAVALMFAGLTLANILGVPGGTALGEAFGWRATF
                     LAVVVLGLFSVAAIAWLVPSNVAEPSGGGLRSEVRVLAKLQVWLAMLISALASASLFA
                     VFTYIKPYLTDVSGLSTSAVTWVLLLFGGGMTIGNVIGGRLADWKLMPTVIGTLAMLT
                     VVFVCFAQFGAMAGVAIGIVFLWGMLVFVIVPPLQIRVVETASEGPNLAATLNQGAFN
                     IGNAAGAWVGGLALSFGVSYANLPLVGAVVCLLALGVAIVSYTIERRRPMVADAALTC
                     PAQ"
     gene            19512..>19693
                     /locus_tag="VGM46_01500"
     CDS             19512..>19693
                     /locus_tag="VGM46_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M14"
                     /protein_id="HEY4191296.1"
                     /translation="MRKSIERLEGDSAGIAYEFPVFRFDGSDKSAPAAYLQAALHGGE
                     LPGTVAIDALMPKLAKA"
BASE COUNT         3466 a         6500 c         6036 g         3691 t
ORIGIN      
        1 catcgtcaat acggtgatcg ccggctggca tggccgcaag aagcgcggca tcggcgagaa
       61 tttctggcag ttccgcccct atgtcgaggg tgattcctcg cgcatcgact ggcgccgctc
      121 cgcccgcgac gaccatacct atgttcgcga ccgcgaatgg gaagcggccc atacggtatg
      181 gctgtgggcc gacccctcgc cgtccatgct ctataaatcg acggcggcga gcgtctccaa
      241 gcaatcgcgc gcgctggtgc tggcgctcgc catggccgag cttctgtcgc gcggcggcga
      301 gcgcatcggc tggcccggcc tgaccgaccc ctttacggcg cgcaacggcg ccgaacgcat
      361 cgccgcccat ctttcccatg ccgccgatct gtccgccaag cccgaccttt ccggcatccg
      421 ccgcttttcc gacgcggtgc tcatcagcga ctttctcgat ccggtcgagg aaaccttcca
      481 atggctggac gaactggccc ggcgcggcgt tcgcgcccat ctggtcgagg tggcggatcc
      541 tgcggaggaa agctttccct atgcgggccg caccgaattc accgatcctg aaaccggaga
      601 aaggctgacc gccggccgcg ccgagatgct gggcgacgac taccgccggc tttacgccgc
      661 gcgtcgcgcc gaattggcct cccggtgcca gcggcttggc tggagcttca ccaccaacca
      721 caccgaccgg ctcgcctccg acgcgctggc gcggcttcac ttggcgatgg gcggcgacag
      781 cctccatgcc gggaagatcg gcgcatgagc tggctgccgc tctcctttgg cgcaccgatg
      841 gtgctgtggg gcctcctcgc cctgccggtc atctggtggc tgctgaaatt gacgccgccc
      901 aagccgcagg cggaggtctt cccgccgttc aaaatcctgg cccgcgtgct gaaacgacag
      961 gaaaccccgc agcgcagccc ttggtggctg acgctgctgc ggctgttgat ggccgcgctc
     1021 gtcatcgcag cgctggccga acctgtattc aacccgcgcg aaaccctgcc cacccaaggc
     1081 agcgcactcg ccttggtcat cgacaatgac tgggccgccg cgcccgactg ggacaagcgc
     1141 gtcgccaccg ccgagcggct gatttcggac gccggctcga agagcatgcc ggtcgtcgtc
     1201 gccctcaccg ctgaaaaacc gaatgccgaa atcggcccct tcgacgcggc agccgcgctt
     1261 gaccggttgc gcgccgccaa gccgcgcccc gtgcccaccg accggcccgc catctatgcc
     1321 cgaattgccg aaacgctggc caccctgccc ggcgccagcg tcgccatcct gtccgatggg
     1381 ctggccgcca agggtgacga gacggcgttc aagacgctgc tttcgaaaaa cgccgccaat
     1441 gtcgtctggg ccgaaccgga acgagttgcc gccatcgctc tcaccagtgc cgaaaacgag
     1501 gtggacggct ttgcgctgac cgccatccgc gcgccgggcg agcagacagc cgcaaaggtc
     1561 accgccggcg ccttcgacga caagggccgc cgcatcgccg acgccacgct gagcttcgcg
     1621 cccggcgaaa ccaagggcac cggcacgatg gccgtgccgt tcgaactgcg caatgatttc
     1681 tcgcagatcg cgatcgatgg cgagcgccat gcgggcgcgg tgcgcgtgct ggacgacagc
     1741 gccaagcgcc gccgcgtcgg gctgttgtcg caagccgagg ccgaccaggc gcagccgctt
     1801 ttgtcgccgc tctactatat tcgccgcgcg ctctcgccct ttgccgatct ggtcgagcct
     1861 tcgagcgccg atcttgccga cgccattccg caattgctcg accggaaacc ggcgatgatc
     1921 gtcatggccg atgtcggcgc aattccccag caggtccggc aaaggctggt cgactgggtc
     1981 aataatggcg gcacgctggt gcgctttgcc ggctcgcgcc tcgccgccgc cggcaatgac
     2041 gacgatttgc tgccggtgcg cctgcgcacg ggcgaacgtt cgctgggcgg cacgctttcg
     2101 tggaccacgc cgcagccggt ggccgaattc cccaaaaccg gcccgttcgc cgacctcgcc
     2161 gcgccgaagg aagtcaccgt aagccggcag gtgctggccg aacccggccc cgacattgcc
     2221 gaccgcacct gggcaagcct tgccgacggc acgccgctgg tcaccggcct gaagaagggt
     2281 aagggcacgc tggtgctttt tcacacgacg ccggaagcga catggtcgaa cctgccgatt
     2341 tccggcagct tcgtcgaaat gctgcgccgc atcgtgcaat tgtcgcgcaa ccagggcgcg
     2401 gtggcttcca ataccgaaat caccgccacc gccctcgcgc cttatcgcat gatcgccgcc
     2461 gatggcgcgt tggtgccgcc gacgcccgat gcccggccgc tggttcccaa cgccaaggct
     2521 cccgtcacgc ttgaaaaccc gcccggcctc tatggctccg acgccggcgt ctatgcccac
     2581 aatctgctcg atgccgacag cacgctgacg ccgcttgcgc gcccgaacat ctcggtgcct
     2641 gtcaccgcca tgcgctacgc cttcgatgaa tcgcgccacc tgaaaggctc gctggttctc
     2701 gcggcgctgg cgttgatggt tctggacacg ctggcggtgt tctggatggg cggcctgttc
     2761 tcacgccgca gcagtgcgcg ccggctttcc tccgcgacga cggcagccgt cgcactggtc
     2821 gcgcttgctg ctctctccgc ttatcccggc agcgcgcgcg ccgatgattc caagccgggc
     2881 gacgcgcagg ccatcgaggc cgtcgccaag acgcgcatcg cctatgtgct gaccggtgaa
     2941 ccgggtgtcg acgccatcag ccgcgccggc atcgaggggc tggatcgctt ccttatcgaa
     3001 aagacggcgc tggaaccggg cgagccggca ggcgtcgaca tcgccagcga tgagttgtcc
     3061 ttctacccgc tgatctactg gcccatcgat gccgctgccg cgatgccgag cgaggcggcg
     3121 attgcccgca tcgacgccta catgcaacag ggcggcacgg tgctgttcga tacgcgcgac
     3181 cagttcgcca ccggcatcgg cgccgattcg gccagccccg cgacgacccg gctgcgcgac
     3241 attctgggca atttgaacgt gccgccgctg gaaccggtgc cggccgacca tgtgctgacc
     3301 aagtccttct acatccttcc cgaatttccg ggccgcttca acggcagccc gctctgggtc
     3361 gaagcctcgc tcgatgccgg caatgcggac aaccgcccgg tgcgcaccgg cgacggcgtt
     3421 tcgccgatca tgatcaccgc caatgatttt gccggcgcat gggccatcga cgccaatggc
     3481 gatccgctgc tgcccaccgt gccgtccgac ccgctacagc gcatttacgc cttgcgcgcc
     3541 ggcgtcaaca tcatgatgta catgctgacc ggcaattata aatccgacca ggtgcatgtg
     3601 ccgatcctgc ttgagcggct gggacagtag catgaactgg tcgctcgcct tcgaacccct
     3661 gttctcgtgg ccatggcttg cagcggccct ggctccgctg gccatcctcg cgattatcgg
     3721 cctgtggttt cgccagcgcg gcggatggct gcgcgcgctg gccctggccg cccttgcggc
     3781 ggccctgctc aaccccgtct tcctcgacga agaccgcgag ccgctgaaaa gcgtcgtcgc
     3841 cgtcgtggtc gatcgcagcc agagccagga cattggcgaa cgtaccgcac aaaccgacgc
     3901 cgcccttgcc ggcgtcaagg aacggcttgc ccgcttcaag cagttcgacg tgcgcgtggt
     3961 cgaaaccggc cgggccagcg ccgccgaaga ccgcaccgaa acgcggctgt tttcggcgct
     4021 cgacagcgcc ttccgcgatg tgccgccctc gcgcatcggc ggcgcggtga tgataaccga
     4081 tggcgaggtg cacgatgtgc cgaagggcgc gcccgaattc gcagcgcccc tgcatgccct
     4141 gattaccggc aacgaccatg aaaaggatcg ccgcatccgc tttgagcgcg cgccgcgctt
     4201 cggcctcgtc ggcaagccgc tcgacatgtc cttccgcgtc atcgcctccg acaatcagtc
     4261 cggcccggtc gatgttcgcg tctcggtcaa tggccagcag gtagcggtcg aacatgcctt
     4321 catcggccag gaaatgccgt tgcaggtcac catcccgaat gccggccgca acataatcga
     4381 gcttgccatc cccaagcagg aaggcgaact gaccgaaatc aacaaccgcg ccatcgcgct
     4441 ggtcgacggc atccgcgaga atttgcgcgt gctgctggtt tccggtgagc cgcacgcagg
     4501 cgagcgcaca tggcgcaacc tgttgaagtc cgacgcctcg gtcgatctgg tccacttcac
     4561 catcctgcgc ccgccggaaa aacaggatgg cacccccatc aacgaactgt cgctgatcgc
     4621 ctttcccacg cgcgagcttt tcgtggaaaa aatcaaccag ttcgatctca tcattttcga
     4681 ccgctaccag caccgcgacg tgctgccgat cctctattac gattacatcg ccgaatatgt
     4741 ggaacagggc ggcgcattgt tgattgccgc cgggccggaa tatgccggcg agcagaccat
     4801 tgcgcgcacg ccgttgatgg cggcgctgcc ggcgatgccg accggcgagg tcatcgacaa
     4861 ggcgttctac ccgcgcctga ccgatattgg ccagcgccat ccggtgacgc gcggcctaga
     4921 tggatcgggg gtcgagccgc cgcattgggg ccgctggttc cgcaccatcg gcgtcgagaa
     4981 accggaaggc gaggtggtga tgaagggcgc cgacaaccgg ccactgctgc tgctcgacca
     5041 caagggcgaa ggccgcgtcg gcatgcttct gtccgaccag ggctggctgt gggcgcgcgg
     5101 ctatgaaggc ggcggcccgc atgtcgagct ttaccgccgc atcgcccatt ggctgatgaa
     5161 ggagccggaa ctggaagagg aaaggctgac cgccgatggg cgcggcatgg tgttggaagt
     5221 gcgccgccag accatgagcg acgcccccgg cgcggcgcaa atcatcacgc cgtcgggcaa
     5281 ggcgctgacg gtcaagctgc aaaaatccga acctggtgtc ttcaccggca gcgttgaggt
     5341 caaggaaatc ggcctctacc aggtcggcaa cggaaatttg acggcgctcg cccatgtcgg
     5401 gccggtcaat gcgcctgagt tcacggatgt ggtttccacc gaaaacaaac tgaagcctgc
     5461 gacagatgcg accggcggca gcgtccgccg cctggccgcg tcgtcgggca atggcgtggt
     5521 tttaccctcg atcgtaccgg tgcgctcatc cggcgaagcg gcaggcaacg actggatcgg
     5581 ccttcgcgcc accgacgaca gcgtgctcaa ggcagtcagc cgcgtgccgc tgttcggcgg
     5641 cttcctcggc cttggcgtgc tgctgctggc catgggatcg atgtggtatc gcgaggggcg
     5701 ctaggcccac tggctcccgg aacctaagcc cctcccgcga gttgcttttc cagtggaagc
     5761 tcactcgatg gagaagttga atggccgagg acaacgatcc gcagcgcgcc tgggatttga
     5821 tcgaaaagat cggtttttgc atgctggcga ccaatgacgg cagggatatt cgctcccgtc
     5881 cgatgacggc gcacgccgag cgcgaagaaa acgccgttta ttttctgctc gacctgaaag
     5941 accacaaaaa tctcgacgcc ttcgccagtt cctgcctggc tttcgccgat tcgggcgaca
     6001 tgaactacgt gtcggtcacc ggctccggcg aggtgtccaa cgaccgcgcc aagatcgggg
     6061 agctttggtc gtcgccctat aaggcgttct gggacggccc cgacgatccg aatatccgcg
     6121 tgctcaagat cacgccgaaa gacgcccaat attgggatgg tccaggcaag atcgccagct
     6181 atgcaaagat gctggccgcc gccgtcagcg atgctaagcc cgaccttggc gaccatggca
     6241 aggtgagaat gtaacctgcc gggttccact tgggagccgt gacggtatcg acgcttacaa
     6301 agaaggccgc tccggcgtcg atgcgcggag cggcctttga aaaatatgct gcctgctcag
     6361 tacttcttgg caacatgcat cttgtgggcg ctgtgatgaa cagcatgctt cttgtgggca
     6421 cggtgatggg tcatgtggcc gctggcatga tgcatcttgt gggaaacgtg atgcttgtgg
     6481 tgatggacca cggtgtgatg atgatgaacc ttcctgtggg acgaaggagt gtgcatcgag
     6541 gcggcgctgg cgctaccggc gccgagcatc ggagccgaga atgcaagagc gattgcaagg
     6601 ccgagagcgg aaacgaaaga ttttttcacg atatgattcc tgttgtttgc cggtcgggtt
     6661 gatccggtcg aactctgatg cgtccgctat gttcggccgc cacgcaggca gttatgccac
     6721 aggattcttt cgccggtttt tccgaactgt tgaattttgt ttccgaattg ttgctggaag
     6781 ccgttggcgg caggcatttg ccggcggctc tgccttcatc gtgccaggca acgccatcga
     6841 aaaggcagca cgcagcttgc ctgctctatt cctcaggctc cgccgtgaac atcagcggat
     6901 agcccttcgc cttgccggca tcggtagccc gttgcgcctt tgtttcggcc acgtccttgg
     6961 tgaagacggc taccacgcag acgccgcgcc ggtgcgcggt gatcatcacc cgatgggcct
     7021 ggtcctcgct cagcttgaac tcccccttca gcaccgtgac gacgaactcg cgcggcgtga
     7081 aatcgtcatt gacgagaatg accttgtaga gcttcggccg ctcggtcttg ggctgcggct
     7141 tgacgcgcgc gatggtcagc ggatcgttgg tcggtggcga catgacacgc tatttcatca
     7201 tcgccgacgc tcaattcaag cccccgccat cctggcgatg acgaatgctg gaaggccgcc
     7261 ccgcgaaaat ttcgtggagc ggccttggat cgtcagcgaa ttacatcgcc ttcttggcgg
     7321 ccttcttcgg agcaaccttc ttatgggcgc tcttcttgac gaccttcttc tgcgcaacct
     7381 tgtgcacggg cttcttgacg gccggggcgg tcgtcgcggc tgccggagcg gcggcagtgg
     7441 tggtggcggc agcgttggcg gcgctcatgg ccggaacgga gaatgcaagc gcaacggcaa
     7501 cgccgagggc ggagaggagg gacttgttca tggacttttt cctttgttcg caggttgtgg
     7561 gttgagtttc actggttcgg gtctgccggc gacactccca gcactttcaa ggcttgtgcg
     7621 agtatccgga tgccctgcgc ctgttttcta tcggcacaga accggttcgc tttcctctga
     7681 agcgcctttg cctcggcgac ctgcaccgcc ttggcatggg tttccatggc ttcgtcgacc
     7741 tgtcgactta gccggctgca acgttcgctc cgggtgacca gcaccttcga actggccact
     7801 ttcgcatccg attgcccgac agttgcggtg atgagcagaa cggggagcaa cgcactcacc
     7861 aatcttgtcg ggtatctttg catggccggg tcctgaaacc ccctggagcg agtgcgcggt
     7921 cgatggcctg accgccacgg caccccttat gaccccggtt tctttcgcca gtttgtctgc
     7981 aatgttgaat gttgtttccc gattgtttcc ggcttcccgc ccggcagcaa accggacgcc
     8041 acaggcatat ttttgccatc aatgacgccc tatggagaca gcagaccgag catgcagggc
     8101 acgcccttga aatccgatcc gcacatattg atcgtcgatg acgacaagga catccgcgac
     8161 ctcttgcagg aattcttccg caagcgcggt ttgcatgcgt cggtcgccgc cgacggcgtc
     8221 gagatggaat cggtgatgcg ccgcgcgcag atcgatctca tcgtgctcga cgtcatgctg
     8281 cccggcaaaa gcgggttgga actgtgccgc gagcttcgcg cccgctaccc gacgccgatc
     8341 atcatgctga ccgccgtcac cgaaacgacg gaccgtgtgg tcgggctgga aatgggggcg
     8401 gacgactatg tgccaaagcc gttcgacccg cgcgaattgc tggcacgcgt ccgcgccgtg
     8461 ctgcggcgca acgaccccga aacccgcaag gagccgcaac ggcaggtcta tcgcttcgcc
     8521 ggctggagca tggattgctc gcgccgtcgc ctgatggcgc caggcgacgt actggtcgag
     8581 ttgacgggag cggaattcaa cctgcttcac accttcgtca ggagtgccca gcgcgtcctg
     8641 acccgcgacc agctcattga actgtccggc ggtgaaggcg gcgccagcta cgaccgctcc
     8701 atcgacattc tggtcagccg gctgcgccgc aagatggaag acgatccgcg cacgcccaag
     8761 cttatcatga cagtgcgcag cggcggttat cagttcatgc cggaaacggt agccgaatga
     8821 ggcgtttcct gccgcagacc ctgcccgcct gggttctgct cgtcgtcatc accggccttt
     8881 tggtcagcca gttgaccact ttctacctcg tccagcgcga ccgcgccgcc agcaacgatg
     8941 tggtggaact gtaccgcctt aacgagcgta cgttttccta tgtgcagctc atgcacaagg
     9001 cctctccccc ggaccgcaag cagctggcgg cgggactctc caacagcggc aacgcgctga
     9061 cggtttccga tacgccggtg gtttcctcac cgatcgccac cgatgattca ctggccgagc
     9121 ttgaggacat tctggtcggc cgcctttcga aattcggcgt catcgatgcg cgcgtgcgcc
     9181 gcgatgccgc cgaacgggac gcgaccgggc ttggccagac ggacacggat acggaacttg
     9241 gccaggtcga gcgcgacctg ctggcgctgg cgaccaattt cgccaagagc aagaaactca
     9301 ccgcctcggt cgaattcgcc gatggccaat ggctgaactt caccaccccc atcacgccgg
     9361 tcgcaccggt gctgaacctc gacagcacgc cgctgtcggc catcgtcgcg gcgctggtgg
     9421 tcatcatgtc gatctggtcg ctgcgccggc tgaccgcccc ctaccggacg ctggagcgcg
     9481 cggtcggcaa gatttcagcc gatctcagaa gcccgccgct cgatgagaac ggcagccgcg
     9541 aataccgttc cgccgccagg gcgctgaatg ccatgcagtt gcgtctgcgc gaatatgtcg
     9601 aagaccgcga acagcttgcc gccgcccttg cgcatgattt gcgcacgccg cttacccgca
     9661 tgcgattgcg gctggaattg cttggccggt cgaaagcccg caccgccctt gcgcacgacc
     9721 tgtccgatat cgaccagatc gcgcgttccg tcatcgattt cgccaccttc gaaatggccg
     9781 aggaaaagcc ggagcgcatc gacctcgctt cgctcgcttc gtcgattgcc gacagttacg
     9841 acacggtgtc gttcgaacgc gaagccgagc ggggcatggt ttgcgcagcg cgcccgatag
     9901 cgctgcgccg ctgcattacc aacctggtca acaatgcggt gctctacggc aagcgggtgc
     9961 atctcgatct tcaccgcaat ggtggcaaca tcgccctgac gatccgtgac gaggggccgg
    10021 gcatcgcgcc cgagcagttc gagcaggtgt ttcggccttt cgttcgcatc gagacatcgc
    10081 gcagccgcga aaccggcggc tccggcctag gcctgaccat cgcccgcaac atcgcccgca
    10141 gcatgggtgg cgatgtgcgg ctgagcaacc acccgctagg cggcctctgc gcggagctga
    10201 cattgcccgc cattaccgta acggcgtgag catcggcccc agccagctcc ggctcgcttg
    10261 gcgaggtcgg tttcgaacac acgaggcgga acgaaatcga gccggcccgg caggcacgcc
    10321 gcatctgctg cgacattccg attcaagatc aaccgcttgc ggcggttctc cgaatcatct
    10381 tgtcaacggc ctaacgccgc agcaccgccg acaaaacgtc ccggatgccg atcgagccga
    10441 cgaagcgcga ctgttcgtcg aacagcgcca caggcgccgt ctgtgagcgg tgcatggcca
    10501 gcatgaccgt cttcaacgac gtgccgggcg ttgcccagaa cacctggctc gcctcttcgg
    10561 gctggccctc gacatcggcg cacgacaccc agatcgcagg cttgccgttg cgttccgcag
    10621 ccgacaccag ccccttgcca tcgaccttga aacgggtggt ggcgcgccgg tcgagccaga
    10681 cccagccatc cccgctttcc tccagctcgc gccggtcgcg catcacgttc caggcggtca
    10741 gcaccgacaa cggattaacg ttggcgatga attcgcgcac ataatcgttg gccggccgca
    10801 gcacgatgtc ttcgggcgcg ccggtctgca cgatccggcc gccttccatg atcgagatat
    10861 gggtcccgat cttcagcgct tcttcaaggt cgtgggaaac gaagatgatg gtcttcttca
    10921 gttccgcctg caattgcagc aattcgtctt gcagcttggt gcggataagc ggatcgagcg
    10981 ccgaaaacgg ctcgtccatc aaaaggatcg gcgcttcggt ggcaaaggcg cgcgccaggc
    11041 cgacgcgctg ctgcatgccg ccggaaagtt cgtgcgcgta tttcttcgcc cattgttcga
    11101 gattgacgag cttgagttgc ttgtgaacgc gctccttgcg ctcggcctcc ggcacgccgg
    11161 caagctcaag cccgaagccg acattttcct ccaccgtgcg ccatggcagc agtccgaatt
    11221 gctggaacac catcgccacc tgcttctggc ggatggcgcg cagcgtcgtg gcgctacaat
    11281 tgacgacatc gaccgcgctg tcgccctgct tgacgaaaac atggccccgc gccggcacgt
    11341 tcaaaaggtt gacggcgcgc agcagcgtag acttgcccga ccccgacagg cccatcagca
    11401 ccgaaatatc gccttcctgg acggtgaggt tgcaattggc gcagccaagc accgcaccgg
    11461 tctgcgccag gatttcctgg cgcgtcgcac cggtgtcgat catggccagc gcccgtggct
    11521 tctcggttcc gaagacgata tcgacgttct tgaaatcgac cgcgacgttc atttcctgcc
    11581 tccggtggta acgcggctca tgcggtcgag gatgatggcc aaaacgacga tggcaagccc
    11641 ggattcaagt ccgagatcta tcctgcgcga accaagtgcg cggttgattt cggtgcccaa
    11701 cccgccggcg ccgatcagcg cggcgaatac caccatcgac agcgacagca tgatcgcctg
    11761 ggtgaggccc gccatgatgg tcggcaatgc ggccggcagt tccaccttcc acaaaagctg
    11821 gcgcttggtc gccccaaatg cctggcctgc ttcgacgatc gattttggca ccgaggtgac
    11881 gccgagatag gtcagccgca ccggggtcgg gatgacgaag atgatggtaa cgaccaggcc
    11941 cggcgcattg ccaagtccaa acagggtcaa taccgggatc aggtagacga aggtcggcag
    12001 cgtctgcatc atgtcgagga ccggcagcat ggcgcggtag actttcggct tgtgcgcggc
    12061 ccagatgccg agcggcacgc cgatcgccat cgacaccgcg gcggcggcca ctaccagcac
    12121 catggtttcg accgtctgct tccacaggcc ttggttgatg atgaagacaa ggccgatgaa
    12181 cacccagacg gcaagcggcc tggaacgctg caatagccag gcaaggccgg caatcaccag
    12241 cgccagcaaa actggcggcg ctgccagcag aagatcgacg agattgtcga ggagcaggtt
    12301 gaggccgctc gaaaaggccc ggaagatcgt atcgaaattg tcggtaagga agccgaagaa
    12361 atccttgccc caggagccta tcggtatctt gtagctggcc aggaatttcg aaaccggatc
    12421 catctttcct ccttttgccg cctttcccga accgaaccgg cctcgggaca ggatcgcaaa
    12481 gtcgatctaa acatatcgct gccggtaagg ccggccgcat caggcgcggc gacccaacgc
    12541 tcacccgtca catccgccgg cctatccccg gaccgtcccg ccggatcatt gtgtcacggg
    12601 atgcgaaaaa gggcagccct tgcgcaaagc tgcccttttt atcgttacgc cgaacgccgg
    12661 ctcagctgcc cagcgcggtc ttgacggcgg cagcggcgtc tccgccgtcg atcgtcgtca
    12721 cgccggccag ccagggggtg accgcatcgg ggttcttttt cagccagtcg gtcgccaccg
    12781 tgttggcgtc gccgcccttg aggatggcgt ccatcatctg gccttccatg tccagattga
    12841 acttcaggtt cttgatgaac gtgccggcat tagggcattc cgtcgtgtag cccttgcgca
    12901 cattggtgct gacggtggcc gcgccgaagc cggaatcgcc catcccgtcg agataggtga
    12961 tcttcatcgc gcccatcacc ggatgcgggg tccagccgag gaaagcgatc cattcattgt
    13021 tcttcatcga ctgttcggcc tgcgtcagca tgccggcctc ggaggattcg accagttcga
    13081 aaccgtcgag attgtcggcc gggttcttga tcatgtctaa gatgatgcgg ttgccgtcat
    13141 taccggcttc gatgccgtag atcttgccgt tgaacttgtc cttgaacttg ccgagatcgg
    13201 tcagcgtctt gacgccggcg tcggccacat aggtcggcac aaccaggccg tagcccgcgc
    13261 cggtaagatt ttcactgatc gtctcgaccg agccgtcggc agcgtaatcc ttgatgtcgt
    13321 tggtcatcga cggcatccag ttgccgagga aaacgtcgag atccttgttt ttcagcgaag
    13381 cataggtcac cggcaccgaa agctggatga ctttcggctc gtagccgagc gcggtgagca
    13441 acaccgaggc gaccccggtc gttgcctgga tatcggtcca gccgacatcc gaaaggcgta
    13501 ccgtcttgca gctttcagga tcggcggcaa aggcgacgct cgtggaaagg aacgccgcca
    13561 ggcccaggcc ggcgacgaag gaattcatgc gcgacatcgt gacatctccc atttgcgatt
    13621 ctttggcgaa gcggatttcc gtatccgcct tgtttttgca gccaaacatc atttaggggc
    13681 ggtttcaagc gccaaagcaa tcgtgacggg cggcgcgcac ttgccgatca acgacatggc
    13741 ccgcgctttt ttccggggaa agccggcccc acccctcccg cgatggagag gcttgagcgt
    13801 agaacgggcc atggccaagc tctatttcca ctacgccacg atgaatgccg gcaagaccac
    13861 gatgctgttg caggcgtcgt acaattaccg cgagcgcggc atggagacga tgctgttcat
    13921 cgccggccac taccgcaagg gcgacagcgg ctgcatttcc tcgcgcatcg gactggaagc
    13981 cgaagcggag atgttccgcg acggcgacga tctcttttcg cgcatagccg aacatcgcag
    14041 ccgcacggcc gtgcattgcg ttttcgtcga cgaagcccag ttcctggacg aggaacaagt
    14101 atggcagctt gcccgcgtgg cagaccggct cggcatcccg gtaatgtgct acggcctgcg
    14161 caccgacttc cagggcaagc tgttttccgg ctcgcgcgcc ctgctcgcca ttgccgacga
    14221 attgcgcgag gtgcgcacca tctgccgctg cggccgcaag gcgactatgg tggtgcggct
    14281 gggcgaggac ggcaaggtgg cgaagctggg cgagcaggtg gcgatcggca aggatatcta
    14341 cgtttccctg tgccgccgcc actgggagga ggaaatgggc agggccgtcc cggacgattt
    14401 catcggcttc actcatgggt gaaaggccgt cttcttccca atcagtccgt gcaatccgcg
    14461 ccaaaacctg ttagaacggc ttccgtccac ctatatgttc gatgaaactc accgagccgg
    14521 cgaacgtttc ggcgcggtat tttgtttccg atcaaacacc cagctagaag aaggaaagcc
    14581 attgcaggtt cttgtacgcg acaacaacat cgaccaggcc ctgagggtcc tgaagaagaa
    14641 attgcagcgc gaaggcgtct tccgcgaaat gaaggcgcgc cgcgcctatg aaaagccatc
    14701 cgaaaagcgt gcccgcgaaa aggccgaagc cgttcgccgc acccgcaagg ccgcccgcaa
    14761 gctggccatc cgcgaaggcc tgatcgccgc gccggtgaag aagctgcccc tgcggcccga
    14821 ccagcgcacc gcccgccctg cggcgtaaat cccggttcgc caatccgccg cccggataaa
    14881 ccggcggcgg atgtcgtggc gggtgccctc cccaggcttc ggcaacattg cgggcttcgg
    14941 caattttttg ccccggttgt agaccctctc aaaaccgcat atctgaagca aggcagcgtt
    15001 tcatctgccc tgcggcctgt atgactgaga gggacccgac gccatgcttg atccgatcgt
    15061 gaacttcgtc acccggattt tcggcctcct tggccgcgcc atcggtttcc tggtcggtat
    15121 tgtcctgtgg cccttcctgt ggctgggccg ctggtacgcg cagcgcggct ggatgctgaa
    15181 agcggcgctc ggcgcggtcc ttctgatttt gatcggcctg tacgccaatt tcttctacgc
    15241 cacccaatgg tggaacaatt tcaatcccga ctatccggca gccatgcggc agcagaaaag
    15301 cgtatccgtt gccggcggcc ccgttgccgg caataccggc gccgctgcga ccggcagcgc
    15361 ttcgcaggcg ccggcatcct gccagccatc ggccatagca ggcatttccg ccgaactgat
    15421 cgacttcaac gtcaaccaga attcctgggt gtcctcgatg gtcgtgtcga agatgggctt
    15481 cttcggcatc gactggcggc gcacgccgtt cttcgacaac aaggccgcgt tccagcttgg
    15541 catcaaccag gtcatgcggc gcactaccac cgaactggtc gacacgctgg gtcgcgtgcg
    15601 cggcacctcg cagatcgacc agaacctaca ggatgcgcgc acggcgctgg cctggaacga
    15661 ggatgcctgg tatctcggct ggcgcggccc tacccgcccg acgcccagcg tttaccgcga
    15721 agccatcggc aagctgcgcg cctacaacac cagccttgag aaatgccagg tcaccttcga
    15781 cgcgcgcgcc gacaatctga tgcagttcct cgaccgcatc gccggcgaca tcggctcgac
    15841 ctccgacatc ttgcgctcgc gcatcgatgc ttcggacgcc ggctggttcg acctgcgcgc
    15901 cgacgaccgt ttctggttcg cctatggcca actctacgct tattacggcg tgctggttgc
    15961 ggcgcgctcc gactttgccg ccgtcattgc cgagcgcaac ctcaacacgc tgtgggcgcg
    16021 catggaggag cagtcccgcg ccgccctcaa cgtgcagccc tggatcatct cgaacggcag
    16081 cgaatcgagc gccttcttcc cctcgcatct ggccacgatg ggcttcaata tgcttcaggt
    16141 tcgctccaac atcgtcgaga tccgctcggt gctcgaccgc tagccgagcc aaccggcgcg
    16201 tcttcccaaa cgccggccct atgttatcct gacagggcgg cataatcggc cgttcatctt
    16261 atcggggcgc gaatgacgat ctacctgctt gcattgttga tcggcgtgat cgccggcctg
    16321 agggcattca ccgcgcccgc cgccatcgcc tggggcgctg gccttggctg gctgtcggta
    16381 gccggcacat gggccggttt catgggccat tggatagcgg ccgccgtctt caccattctc
    16441 gccgtcatcg agctggtcag cgaccaattg ccgtcgacgc cgagccgcaa ggttgcgacg
    16501 cagttcggcg cccgcatcgt cgtgggcgcg ttctcgggcg cggtcatcgg cgctacggct
    16561 ggcctgacgg taggcggcat gacggttggc gcgatcgggg ccgtcatcgg cacgctgggc
    16621 ggcgcggagg tacgggcgcg attggccgca gccttcggca aggatgcgcc cgccgccttc
    16681 atcgaggatg ccgtcgcaat cgtcgtcggc ctgcttatcg tgcacgccgc ctcatgacag
    16741 ccaggaaatt cgacgccatc atcgtcgggg ccggccaagc cggcccgccg ctcgccggca
    16801 ggttgacggc ggccggcatg accgtagcgc tggtcgagcg caagctggtc ggcggcacct
    16861 gcgtcaacac cggctgcatg ccgaccaagg cgatggtcgc cagcgcctat gccgcccatg
    16921 tcgccggccg cgccgcccat tacggcgtga cgatttccgg cgctgtcggc atcgacatga
    16981 agaaggtaaa ggcggtcaag gacaaggtgt cgaacgacgc gcgcaccggg gtggaggaat
    17041 ggatcgccgg catgaagggc tgcacgctgt ttcgcggcca tgcccgcttc gagagcgcca
    17101 accagataag cgtcggcgac gatgtgctga gcgcggaaaa gattttcctc aacaccggcg
    17161 ggcgcgccac ggtgcccgac ctcgccggca tcgatacggt cgactatctc accaattcct
    17221 cgatgatgga tttggatgtg ctgccgcgcc accttgtcgt tgtcggcggc agctacatat
    17281 cgcttgaatt cgcgcagatt tttcgccgtt tcggctccga agtcacgata atcgagaagg
    17341 gcgagcgcct gatcgggcgc gaggacgagg acgtttcggc ggcgatcaag tcgatcctcg
    17401 aaaacgaagg cgtcaagatc cacctcggcg cggataccgt cagcttcgcc aaaaagggca
    17461 acgacaccgc cgtgacattc cgcgccggcc agccgccggc tatcggctcg catgtgctgc
    17521 tggcgctggg gcgcacgccc aacacgcagg atctcggcct cgacaaggcc ggcatcgacc
    17581 aggacaagcg cggcttcatc gtcgtcgacg attatttgcg caccagcgtg cctggtatct
    17641 gggccatggg cgaatgcaat ggcaagggca ccttcaccca caccgcctac aacgactatg
    17701 aaatcgtcgc ggccaacctg ctcgacgacg atccccgcaa ggtcagcgac cgcatcgaag
    17761 cctatgggct cttcatcgat ccgccgcttg gccgttgcgg catgacggag gcggcggcga
    17821 gaaagtccgg ccgcaaggtg ctggtcggcc aacggccgat gacccgcgtc agccgcgctg
    17881 tcgaaaaggg cgagacgcaa gggtttatga aaatcctggt cgatgccgat accgacgaaa
    17941 tccttggctg ctgcgtgctt gggcctggcg gcgacgaggc ggtccacggc gtgctcgacc
    18001 tgatgtacgc caaggcgccc gtaacgacct tgcggcgcgc cgtgcatatc catccgactg
    18061 tagccgagct tttgccgaca atagcgcagg aactgaagcc tctcggataa gcgacctgcc
    18121 ccgcagcccc gtcccgctat cgctgcaatg ctccattgca gcgatagcgt ttgcgcactg
    18181 atcgccgcca tgcgattgct cgcctaaatc gtttcataat ggacgtttca tgcccctgcc
    18241 gatccttgcg ctcgccattg cgtccttttg catcggcacc accgaattcg tcattatggg
    18301 actgctgccg gaagtggcgg ttgaccttaa agtatcgatc ccatcggccg gcctgctggt
    18361 caccggctat gcgctgggcg tcgttttcgg cgcgccggtc attgccgtcg ccaccgccca
    18421 tatgcggcgc aaacccgttc tggtcgcgct cgcctcgctg ttcgtcgtcg gcaatctttt
    18481 atgcgccatc gcgccgacct attggacgct gatggccgcc cgcgtcatca ccgccttcgg
    18541 ccatggcgct ttcttcggca tcggctcggt ggtcgccgcc agcctcgtgc ccaggaacaa
    18601 gcaggcaagc gccgtcgccc tgatgttcgc cggcctgacg ctggccaaca ttttgggcgt
    18661 gcccggcggc accgcgctcg gtgaagcctt cggctggcgc gccaccttcc tcgccgtcgt
    18721 ggtgctcggc cttttctcgg tcgcggccat cgcctggctg gtgccgtcca acgtggccga
    18781 gccaagcggc ggcggcctgc gcagcgaagt gcgcgtgctg gcaaaattgc aggtgtggct
    18841 ggccatgctt atctcggcgc tggcctccgc cagcctgttt gccgtcttca cctacatcaa
    18901 gccttacctc accgatgttt ccggcctgtc gacctcggcg gtaacctggg tgctgctgct
    18961 gttcggcggc ggcatgacca tcggcaatgt catcggcggc cggcttgccg actggaagct
    19021 gatgccgacc gtcatcggca cgctggcgat gctgacagtc gtgtttgtct gcttcgcgca
    19081 gttcggggca atggcgggcg ttgccatcgg catcgtcttc ctgtggggaa tgctggtctt
    19141 cgtcatcgtg ccaccgctgc aaatccgcgt cgtggagacg gcgtccgaag gaccgaacct
    19201 ggcggccacc cttaatcagg gcgctttcaa catcggcaac gccgccggcg catgggtcgg
    19261 cggactggcc ttatcctttg gcgtttccta tgcaaacctg ccgctggtcg gcgcggtcgt
    19321 gtgcctgctt gcactcggtg tggccatcgt ttcctacacc atcgagcgaa ggcggcctat
    19381 ggttgccgat gcggccctga cttgcccggc gcaatagctg cgagccagca cttgccggct
    19441 ggcccaatcg tcctccatcg acatggctga cgccgtttgg catgtcattg ccctgttcca
    19501 gaggagtatc catgcgcaaa tcgatcgaac ggcttgaagg cgacagcgcg ggcatagcct
    19561 acgaattccc ggttttccgc ttcgacggca gcgacaagtc ggcgcccgcc gcctacctcc
    19621 aggccgccct tcatggcggc gaattgcccg gcaccgtcgc catcgacgcg ctgatgccca
    19681 agctggcgaa ggc
//
LOCUS       DASYCS010000035         3230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2760296,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000035 DASYCS010000000
VERSION     DASYCS010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2760296"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="VGM46_01505"
     CDS             <1..787
                     /locus_tag="VGM46_01505"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018330148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEY4191297.1"
                     /translation="IGLMQHLNDKGINCPLPVQRKDGQIIGELAGRPAAIVTFLEGIW
                     TRRPTAEHCREVGKSLAAMHLAGQDFSLRRTNALSVDGWRPLWDRAAARADEVEPGLA
                     PEVEADLAALEKEWPADLPKGVIHADLFPDNVFFLGKNLSGLIDFYFACWDMLAYDIA
                     ICLNAWCFEKDNSFNLTKGTALLSGYTAVRSISAAEAAALPMLAHGSALRFMLTRLYD
                     WLNTPAGSLVVKKDPLEYLRRMRFHRQVRSASEYGLKMDSVEA"
     gene            784..1341
                     /gene="rnhA"
                     /locus_tag="VGM46_01510"
     CDS             784..1341
                     /gene="rnhA"
                     /locus_tag="VGM46_01510"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010914899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEY4191298.1"
                     /translation="MKRIEIFTDGACSGNPGPGGWGALLRFNDRVKELSGGEAMTTNN
                     RMELMAAISALDALKEPCAVDLHTDSSYVKDGISGWIEGWKRNGWKTAAKQPVKNAEL
                     WQALDAARKRHQVTWHWVKGHAGHPENERADELARMGMAPFKKTLPKAPASPAGSPVS
                     QARVKKPVDAAKPGSKLPRRSTQSY"
     gene            complement(1494..1979)
                     /locus_tag="VGM46_01515"
     CDS             complement(1494..1979)
                     /locus_tag="VGM46_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006330643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY4191299.1"
                     /translation="MTISVGEKLPEATFKTMTADGAKNITTAEIFSGKKVVLFAVPGA
                     FTPTCSNNHLPGYLENHDAILARGVDAIAVVAVNDVHVMGAWARFTGGEGKILYLSDG
                     NGTFAKSLGLDIDLSAGGMGLRSKRYSMIVEDGKVTVLNVEDSPGQAVESGAAKILEA
                     L"
     gene            complement(2060..2863)
                     /locus_tag="VGM46_01520"
     CDS             complement(2060..2863)
                     /locus_tag="VGM46_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019857003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HEY4191300.1"
                     /translation="MQSFKILAAALGFMASALPAFAASSEWSDAEGGRVRLVTAGKPD
                     AAGQIRGALEIDLNPGWKTYWRDPGSSGVPPQFDISRSTNISGAELSFPAPRRHDDGY
                     GKWAGYDRSISLPVVFKLASPGATTAIDADIFLGICETICVPVQASLKLDPAADPDNA
                     DDTALVDAGFAALPEPQRADFGVKPLPGDPETLVVEASAPGDSQAVDFFIAGASDYMF
                     GTPVRGIEGGKITFKVPILDRPTSKPTGSGLHYTLTSADGAVQGLVPYP"
     gene            3021..>3230
                     /locus_tag="VGM46_01525"
     CDS             3021..>3230
                     /locus_tag="VGM46_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008128940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEY4191301.1"
                     /translation="MDVSHQKDAAPTSGFLDDQFLIAMPGMKDDRFARAVIYVCAHSE
                     EGAMGLIVNQTQQMLFPDLLVQLGIL"
BASE COUNT          618 a          984 c         1027 g          601 t
ORIGIN      
        1 tatcggcctg atgcagcacc tgaacgacaa gggcatcaac tgcccgttgc cggtgcaacg
       61 caaggatggc cagatcatcg gcgaactggc cggccgccca gcggcaatcg ttacctttct
      121 cgaaggcata tggacgcgcc gaccaacggc cgagcattgc cgcgaagtgg gaaagtcgct
      181 tgcggccatg catctggcgg gccaggattt ttccctgcgg cgcacgaacg cgctctcggt
      241 ggacgggtgg cggccgctat gggaccgggc tgcggcgcgc gccgatgagg tggagccggg
      301 cctggcgccc gaggtcgagg ccgatctcgc cgcactcgaa aaagaatggc cggccgacct
      361 gccgaagggc gtcattcatg ccgacctgtt tcccgacaac gtcttttttc tcggcaagaa
      421 tctgtccggg ttgatcgatt tctacttcgc ctgttgggac atgctggctt acgatatcgc
      481 catctgcctc aacgcatggt gcttcgagaa ggacaactcc ttcaacctga ccaagggcac
      541 ggccttgctt tccggctaca cggcggtgcg gtccatttcg gcggccgagg ctgccgctct
      601 gccgatgctg gcgcatggct cggcgctgcg cttcatgctg acgcggctct atgactggtt
      661 gaacacgccg gccggcagcc tggtggtgaa gaaggacccg cttgaatatt tgcggcgcat
      721 gcgcttccac cggcaggtcc gctcggcatc ggaatacggg ctgaaaatgg atagcgtcga
      781 agcatgaaac gcatcgagat tttcaccgat ggcgcctgtt cgggcaaccc cggccccggt
      841 ggctggggag cgctcctgcg cttcaacgat agggtcaagg aattgtccgg cggcgaggcc
      901 atgaccacca ataatcgcat ggaactgatg gcggcgattt ccgcgctcga tgcgctgaag
      961 gaaccctgcg ccgtcgatct ccataccgat tcaagctatg tcaaggacgg catttccggc
     1021 tggatcgaag gctggaagcg caatggctgg aagaccgcgg ccaaacagcc ggtaaagaac
     1081 gccgaattgt ggcaggcact ggacgcggcg cgaaagcgcc atcaggtgac atggcactgg
     1141 gtcaagggcc atgccggcca ccccgaaaac gaacgcgcgg acgaactcgc ccgcatgggc
     1201 atggcgccat tcaagaagac actgcccaag gctccagcta gcccggcggg cagcccggtt
     1261 tcccaagcca gggtcaagaa gccggtagac gccgccaaac cgggatcaaa acttccaagg
     1321 cgctcgacgc aaagttattg accggcctgc cggcagggca ggagccgttg ctttcatcat
     1381 gtccgggcct gtcccgggca tctgcgcact cgaactgcaa gaccgtatcc cgatacggcc
     1441 atccgccgat gatgacggcg cgatggctat tcggcaagcg accgatttat cgatcagaga
     1501 gcttcgagga ttttcgccgc acccgattcc acggcctggc cggggctgtc ctcgacgttg
     1561 aggacggtga ccttgccgtc ttcgacgatc atcgaatagc gcttggagcg aagccccatg
     1621 ccgccggccg aaagatcgat gtcgaggccg agcgacttgg cgaaggtgcc gttgccgtcg
     1681 gacagataaa ggatcttgcc ttcaccgccg gtgaaccgcg cccatgcgcc catgacgtga
     1741 acgtcgttga ccgcgacgac cgcgatggca tcgacgccgc gcgccagaat ggcatcgtgg
     1801 ttttcgaggt agccgggcag gtggttattg ctgcaagtcg gcgtgaacgc gcctggaacc
     1861 gcaaacagca ccactttctt gcccgaaaag atttcagcgg tggtgatgtt cttcgcgcca
     1921 tcggcggtca tcgtcttaaa ggtggcttcg ggcagttttt cgccaactga gatggtcatc
     1981 aatgtcctcg ttcaattaaa tcgaatgacg atgcgatagc gaattgccga gcttgcggca
     2041 atcggtgaaa gaggctggcc tacggatagg gcaccagtcc ctgaacggcc ccgtcggcgc
     2101 ttgtcagcgt gtagtgcagg ccgctgccgg tgggcttcga ggtcgggcgg tcgaggatcg
     2161 gcactttgaa cgtgatcttg ccgccttcta tgccgcgcac cggcgtgccg aacatatagt
     2221 cgctggcgcc ggcaatgaaa aagtcgaccg cctggctgtc gcccggcgcg ctggcctcca
     2281 ccaccagtgt ctccgggtcg ccgggcagcg gcttcacgcc gaaatcggcc ctctgtggct
     2341 cgggcaaagc cgcaaacccc gcatcgacaa gcgccgtatc gtccgcgttg tccgggtcgg
     2401 cggcggggtc cagtttcagg cttgcctgca cgggaacgca gatcgtctcg cagatgccga
     2461 ggaagatatc ggcgtcgatg gcggtggtgg caccgggcga cgccagcttg aacaccaccg
     2521 gcaaggaaat ggagcggtca tacccggccc atttgccata gccgtcatca tggcggcgtg
     2581 gcgctggaaa ggacaattcg gcgccgctga tattggtgct gcgcgaaatg tcgaattgcg
     2641 gcggcacgcc gctgctgccg ggatcgcgcc aataggtttt ccagcccgga ttcaggtcga
     2701 tttcaagcgc gcctctaatc tgcccggcgg catccggctt gccggcggtg accagcctga
     2761 cccggccgcc ctcggcgtcg gaccactccg acgaggccgc gaaggcggga agggcgcttg
     2821 ccatgaagcc caaggctgcg gcgaggattt tgaaactctg catggcagtg atttgggcgc
     2881 gccttgatgg cggcgcaacc ggtttgctgc gccgccgcat catatttgcg tgacccgcca
     2941 tcggctgctt accggggttc gccccaaaag acgggaaaac ccatctttaa agccatccgg
     3001 aaacgcgtta ccctttgacc atggatgtgt cgcaccagaa ggatgccgcc cctaccagcg
     3061 gttttctaga cgaccagttc ctgattgcca tgcccggcat gaaggacgac cggttcgcgc
     3121 gcgccgttat ttatgtctgc gcccacagcg aagagggcgc aatggggctg atcgtcaacc
     3181 agacgcagca gatgctgttc ccggatttgc tggtgcagct tggcatattg
//
LOCUS       DASYCS010000036         3587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3050019,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000036 DASYCS010000000
VERSION     DASYCS010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3050019"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="VGM46_01530"
     CDS             complement(<1..690)
                     /locus_tag="VGM46_01530"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEY4191302.1"
                     /translation="MSAEISAHMHPSRSFQGLILTLHNYWAEYGCVVLQPYDMEVGAG
                     TFHPATTLRALGPKAWNAAYVQPSRRPKDGRYGENPNRLQHYYQYQVILKPNPPNLQE
                     LYLGSLAAIGVDPLLHDIRFVEDDWESPTLGAWGLGWECWCDGMEVSQFTYFQQVCGI
                     ECAPVAGELTYGLERLAMYVQGVDNVYDLNFNGREGAEKVTYGDVFLQAEQEYSRHNF
                     EFANTATLLRHF"
     gene            complement(874..2652)
                     /locus_tag="VGM46_01535"
     CDS             complement(874..2652)
                     /locus_tag="VGM46_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006201987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY4191303.1"
                     /translation="MRQASMRWLTGLAVLTGVAASSLPSFARQASEPVEITSFSGAYL
                     AAHIAEADNDLDGAIAYYKQALAFDPDDAALQQSLMLSLISQGRFEESLPYADKLKET
                     PDAERFSRLALAVDSFRKKDFAKAEYWLKLSLESDLDRLIAGVMTGWSKQGAGHGEEA
                     MAFVDKIEGPEWFDLFKSCHSALIADASGLTDKADAAYSKTMQNVAAGGAAPETWMRC
                     AQSYAGFLARKGDKDKALAVLDQADTFGPGKLEIADLRDKINKGGKIEPLVANASDGA
                     AEILLDLATALNRGGGEPFVRLYLEYALALKPDSDAALVQLASVAEQLKDSEKAIELY
                     RRIPENSPLKEASDLQLGLNLADLDKKDEAIAHLKALVDAHPEDMRGYLALGGVYSSK
                     EDFRSAADLYDKAVATLKTPAVANWNIFYQRGIAYERLKQWDKAEPNFRKALELNPDQ
                     PQVMNYLGYSWVDRGMNLQEGLEMIQKAVDLRPSDGYIVDSLGWAYFKLGKFDDSVRE
                     MERAVSLKPEDPVLNDHLGDAYWRVGRKLEATFQWAHARDMKPDPDILAAVQEKLRNG
                     LPPAEAKATAQETPKAKPDLTPAPKG"
     gene            complement(2858..>3587)
                     /locus_tag="VGM46_01540"
     CDS             complement(2858..>3587)
                     /locus_tag="VGM46_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010658340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEY4191304.1"
                     /translation="HDDVVDESGMRRGKKTARMIWGNQASVLVGDFLLGQAFRMMVDV
                     GSLDALDILSSAASIIAEGEVMQLTAAKNLETTEDEHFAVIKAKTAALFSAAAEVGPV
                     IAGASRTDRTALRSYGMNLGLAFQLIDDALDYGGSSQDLGKNVGDDFREGKVTLPVIL
                     AYRRGTATERSFWKDAIENNAADDAGLEKAIGLMTRHSAISDTIGRARHFGEIARDAL
                     APLEATPQKSALIDVIDFCISRVN"
BASE COUNT          647 a         1118 c         1126 g          696 t
ORIGIN      
        1 gaaatggcgc agcagggtcg cggtattggc gaattcgaaa ttgtggcgcg aatattcctg
       61 ctccgcctgt aggaagacgt cgccataggt gactttttca gcgccctcgc gaccattgaa
      121 attcaggtcg tagacattat ccacgccctg cacatacatg gccagccgct ccagcccgta
      181 ggtgagttcg ccggcgacag gcgcgcattc gatgccgcag acctgctgga aataggtgaa
      241 ctgcgatact tccatgccgt cgcaccagca ttcccagcca aggccccagg cgcccagcgt
      301 cgggctttcc cagtcatcct cgacaaagcg gatgtcgtgc agcagcggat cgacgccgat
      361 ggccgccagc gagccaaggt aaagctcctg caagtttggc ggattgggct tcaggatcac
      421 ctgatactgg taatagtgct ggagccggtt ggggttctcg ccatagcgac cgtccttggg
      481 gcggcgcgaa ggctgcacat aggcggcgtt ccacgccttt ggccccaatg cgcgcagcgt
      541 ggtggccgga tgaaatgtgc ctgcgccgac ttccatgtcg taaggctgga gcacgacgca
      601 gccatattcc gcccaataat tgtgaagcgt caggatcagc ccctggaacg agcggctggg
      661 gtgcatatgg gcggaaattt cggcgctcac ggatcgggcc tcggcggcaa tggcaggatg
      721 cgactttccc tagtgccccg caccgtgagc gtcaaggtaa agggccggaa gcgacccaca
      781 cactcgtcat cctcgggctt gtcccgagga tctacaacat ccgtgcgcag atcctcggca
      841 caaggccgag gatgacggcg gagaggggca cgcctacccc ttcggagcag gcgtcagatc
      901 gggcttggcc ttcggcgttt cctgcgccgt ggccttggct tccgccggcg gcaggccgtt
      961 gcgcagcttt tcctgcacgg cggccaatat gtccggatcg ggcttcatgt cgcgcgcatg
     1021 cgcccactgg aaggtcgcct ccagcttacg gccgacacgc cagtaggcgt cgcccagatg
     1081 gtcgttcagc accgggtctt ccggcttcag cgatacggcg cgttccattt cgcgcacgga
     1141 atcgtcgaac ttgccgagct tgaaataggc ccagccaagc gaatcgacga tgtagccgtc
     1201 gctcgggcga agatcgacgg ccttctggat catctccagg ccctcttgca ggttcatgcc
     1261 gcgatcgacc caggaatagc cgagatagtt catgacctgc ggctggtcgg gattgagttc
     1321 cagcgccttg cggaaattcg gctcggcctt gtcccattgc ttcagccgct cataggcgat
     1381 accgcgctgg tagaaaatat tccagttggc gacggccggc gtcttcaggg tcgcaacggc
     1441 cttgtcgtaa agatcggcgg cggaacggaa atcttccttg gacgaataga cgccgcccag
     1501 cgccagatag ccgcgcatgt cttcgggatg cgcgtcgaca agcgccttca ggtgggcgat
     1561 ggcctcgtcc ttcttgtcga gatcggcaag attgaggccg agttgcaggt cggaagcttc
     1621 cttaagcggc gaattttccg gaatgcggcg ataaagctcg atcgcctttt cgctgtcctt
     1681 caactgctcg gcaacggatg ccagttgcac cagggcagcg tcgctgtccg gcttcagcgc
     1741 cagcgcatat tccagataga ggcgcacgaa cggctcgccg ccgccgcggt tgagcgcggt
     1801 ggccagatcg agcaggattt cagccgcgcc atcggaagcg ttggcaacca gcggctcgat
     1861 cttgccgccc ttgttgatct tgtcgcgcag gtcggcaatc tcaagcttgc ccggcccgaa
     1921 cgtatcggcc tggtcgagaa cggcgagcgc cttgtccttg tcgcccttgc gggccaggaa
     1981 gccggcatag gattgcgcgc agcgcatcca ggtttccggc gccgcgccgc cggcagcgac
     2041 attctgcatg gttttcgaat aggcggcgtc cgccttgtcg gtcaggcccg acgcatcggc
     2101 gatgagagcg ctgtggcagc tcttgaacag gtcgaaccat tccggccctt cgatcttgtc
     2161 gacgaaggcc atggcttctt cgccgtgccc ggcgccctgc ttggaccagc cggtcatgac
     2221 gccggcgatc agccggtcga ggtcggattc cagcgacagc ttcagccagt attcagcctt
     2281 ggcgaaatcc ttcttgcgaa acgaatcgac ggccagcgcc agccgcgaaa agcgctccgc
     2341 gtcgggcgtt tccttcagct tgtcagcata gggcagcgat tcctcaaaac gcccttgcga
     2401 aatcagcgac agcatcaggc tttgctggag agcggcatcg tcggggtcga aggccagcgc
     2461 ctgcttgtaa taggcaatgg cgccgtcgag gtcgttgtcg gcctcggcga tgtgcgcggc
     2521 caggtaagcg cctgaaaatg aggttatttc aaccggctcg ctggcctgcc tggcaaaaga
     2581 gggcaggctt gaagccgcca cgcccgtcag aactgccagc cccgttagcc agcgcatgct
     2641 tgcctgccgc atcgtattcc tttcagtcga acgcaaaaac cgccgaagcg gccctgaccg
     2701 tcgaatccac atttgccggc aagcatggcc tttttgcgtt catgcttcaa ccggccggga
     2761 attcacaatc cgggcatggg attaaaacat ggtgcggcag aggctttgcg ctggccaggc
     2821 gcgaggctgc ccttggccga acgcaaccgc ccgcgcctca gttcacgcgg ctgatgcaga
     2881 agtcgatgac gtcgatcagc gccgatttct gcggcgtcgc ttccagcggg gccagcgcgt
     2941 cgcgcgcgat ctcgccgaaa tggcgggcgc ggccgatcgt atcggaaatc gcggaatggc
     3001 gggtcatcag gccgatggcc ttttccagtc cggcatcgtc ggccgcattg ttttcgatgg
     3061 cgtccttcca gaatgaacgc tcagtcgccg tgccgcgcct gtaggccagg atcaccggca
     3121 gcgttacctt gccctcgcgg aaatcgtcgc cgacattctt gcccagatcc tggctggagc
     3181 cgccatagtc cagcgcatcg tcgatcagct ggaaggcaag accgaggttc atgccgtagg
     3241 agcgcagcgc ggtccggtcg gtgcgcgacg cgccggcaat gaccgggccg acttcggcgg
     3301 cggctgaaaa cagcgcggcg gtcttggcct tgatgacggc gaaatgctcg tcctcggtgg
     3361 tttcgaggtt tttcgccgcc gtcagctgca tcacttcgcc ttcggcgatg atggaggccg
     3421 cgctcgacag gatgtcaagc gcatcgagcg agccgacatc gaccatcatg cggaaagcct
     3481 ggccgagcag gaagtcgccg accagcacgc tggcctggtt gccccagatc atgcgcgccg
     3541 tcttcttgcc gcgccgcatg ccgctttcat cgacgacatc gtcgtgg
//
LOCUS       DASYCS010000037         4859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_585922,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000037 DASYCS010000000
VERSION     DASYCS010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4859
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585922"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="VGM46_01545"
     CDS             <1..761
                     /locus_tag="VGM46_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018496096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEY4191305.1"
                     /translation="PLPLEGRTVLITSQSGESAEVVRWFSEAGGVLDIFGLTLEENSF
                     LGRTVPCLVGAGGTELAFAATRSLTISFALHLAVLAALGDDPSTALSALRKPEEVEIT
                     GALEALAEVQTVVTSGRRLQGVAEALALGLTELSRRPCFSLEGGQLRHGPMEMLGPDI
                     GVILFRGDDPTASLVTAMAVSAVETGAPVIVFDCSGAAPVSGAVTLGFSPARGLAAIF
                     AMLPVAQRLMVAFADARVDNAGTPVRSTKITRSE"
     gene            762..1700
                     /locus_tag="VGM46_01550"
     CDS             762..1700
                     /locus_tag="VGM46_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEY4191306.1"
                     /translation="MRPLAVIGNVNVDLILGPAAPWPKAGTEIIVDHDELRVGGQAGN
                     SALVWQALGVDFSIAANVGSDQFGRWLREEFGRRAEKWTVSPEGTTLSVGITHPDGER
                     TFFTTRGHLPRLDLAGVLSVLDGPQLAGGWALLCGSFLTNDLTRDYTAFFDWADSHGI
                     AVALDTGWPMDGWTQANRDAVKAWLSRCALALFNEIETTTLAGLDDPVEAAKKLQAGM
                     KKDAVAVVKRGPQGALAIGAYGAAVQVDAPAVDVVDTIGAGDVFNAAFLAALVRGEAL
                     AACLTAGTQVASRAISTRPRSYGGPLPREELWHEQA"
     gene            1687..2766
                     /gene="ugpC"
                     /locus_tag="VGM46_01555"
     CDS             1687..2766
                     /gene="ugpC"
                     /locus_tag="VGM46_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006204128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEY4191307.1"
                     /translation="MSRLEIRDVRKSYGKAETLKGIDISLADGEFLVLLGPSGCGKST
                     LLNIIAGLTEATSGDIRIGDRSIIGVHPKDRDIAMVFQSYALYPNLTVRRNIGFGLEM
                     RKVPAAERDKAVKEAARLLQIENLLDRKPSQLSGGQRQRVAIGRALVRKPEVFLFDEP
                     LSNLDAKLRLEMRAEIKRLHQMLKTTIVYVTHDQIEAMTLATRIAVMHEGQIEQLGTP
                     EEIYNKPATLYVAGFVGAPPMNMLNVTLAEGVLRIAGSDAAFALPSRFTPDTMANGRE
                     LVAGIRPEALHLDGAGAAPFCFSATIDGAELTGPELVVSARVGSQKVTASLPPRSAIT
                     IGQTRTFAFDEAALHLFDPATGKAF"
     gene            2942..3550
                     /locus_tag="VGM46_01560"
     CDS             2942..3550
                     /locus_tag="VGM46_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018329330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEY4191308.1"
                     /translation="MALLNETTEASSSRQENVERILESAERLFRHYGYGKTTVADIAR
                     DLGMSPANVYRFFASKEQIHQEICGRMLAASLHLALANSKRQLPAAERLRLCILDQHQ
                     LTKETMLDEQKVHEMVIVAIERDWHVIEKHVDRLHQVYSDIIREGIEAGEFRQQDLDS
                     AARCFGAATAALCHPQMVAQCLAKENRAAPDELVDFAIRALK"
     gene            3598..4755
                     /locus_tag="VGM46_01565"
     CDS             3598..4755
                     /locus_tag="VGM46_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018428978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY4191309.1"
                     /translation="MLSFKTFSDFARPLATLSLLGVLGAGVAACTQAKTEPVVEVRPV
                     KVVQIAKADDTRDLDYSGTVKARTEMNLGFRVAGKIVERLVDIGDKVKPGDILARIDP
                     TDYALAQKTAQANLAAAQKQVETAGLTQKRSQQLFAKKFSSQAELDQATLGYQQAIAA
                     RDAAASALQQASNQVAYTDLKADRNGIVTAIGADSGQVVGIGAPVVTVAMDGEKEIQI
                     AVPETDISHFKAGQNVKVGFWTDDELQLSGKVREVAGSADPQSRTFSVRVSLADDPRV
                     LLGMTATVEAKVETKRPLEVIPLEALAKLDGKSIVWVVDHDTSTVHAREIGVAAFAAD
                     GVRVAQGLQAGDLVVAAGTQFMSENLKVKLPDAVAAQGENLDAGGKTAAIR"
BASE COUNT          908 a         1543 c         1553 g          855 t
ORIGIN      
        1 agccgctgcc gctcgaaggc cgcacggtgc tgataacctc gcagtcgggc gaaagcgccg
       61 aggtggtgcg ctggttctcc gaagcgggcg gcgtgctcga cattttcggg ctgacactgg
      121 aagaaaattc gtttctcggg cgcaccgttc cttgcctggt cggcgccggc ggcaccgaac
      181 tggcctttgc ggccacccgc agcctgacca tcagcttcgc gttgcatctg gcggtgctgg
      241 cggcccttgg cgacgatcct tcgacggcgc tgagcgccct gcgcaagccg gaagaggtcg
      301 agataaccgg cgcactggaa gcgcttgccg aggtgcaaac cgtcgtcacc tccggccggc
      361 gcttgcaggg cgttgccgaa gcgctggcgc tgggcttgac cgaattgtcg cggcgtccgt
      421 gcttttcact ggagggcggc cagcttcgcc acgggccgat ggaaatgctg gggccggata
      481 tcggggttat cctgtttcgc ggcgatgacc ccacagcctc cctcgtcacc gccatggcag
      541 tttcggcggt cgagaccggc gcgccggtta tcgtcttcga ttgctcgggt gccgcacctg
      601 tctcgggcgc cgtcacgctt ggcttcagtc cggcccgcgg ccttgccgcg atcttcgcca
      661 tgctgccggt ggcccagcgg ctgatggtcg ccttcgccga tgcgcgcgtc gacaatgccg
      721 gcacgccggt gcgctccacg aaaatcaccc ggagcgagtg aatgcgtccg ctcgcggtga
      781 tcggcaacgt caatgtcgac ctcatattgg ggccggccgc gccatggccg aaggccggca
      841 ccgaaatcat cgtcgaccac gacgaactgc gtgtcggcgg ccaggctgga aacagcgcgc
      901 tcgtctggca ggcgctgggc gtcgatttca gcatcgcggc caatgtcggt tccgaccagt
      961 tcggccgctg gctgcgcgag gaattcggcc ggcgcgccga gaaatggacc gtcagtccag
     1021 aaggcacgac gctttcggtc ggcatcacgc atcccgatgg cgagcgcact tttttcacga
     1081 cacgcgggca tctgccgcgc ctcgatcttg cgggtgtcct gtcggttctc gacggcccac
     1141 aacttgccgg cggctgggcg ttactgtgcg ggtcgttcct caccaacgac ctgaccaggg
     1201 actacaccgc attcttcgac tgggccgaca gccacggcat tgcggtggcg ctcgatacgg
     1261 gttggccgat ggatggctgg acgcaagcta atcgcgatgc cgtaaaggcg tggctttcgc
     1321 gttgcgcgct ggcgctgttc aatgaaatcg agacgacgac gctggccggc ctcgacgatc
     1381 cggtggaagc ggcgaaaaag ctgcaagccg gtatgaagaa ggatgccgtc gcggtggtca
     1441 agcgcgggcc gcagggagcg cttgccatcg gcgcgtatgg ggctgccgtt caggttgacg
     1501 ctcctgcggt ggatgtcgtc gacacgatag gcgcaggcga tgttttcaat gcggcttttc
     1561 ttgccgcgct ggttcgaggc gaggcgcttg ccgcctgcct gaccgcggga acgcaggtcg
     1621 cctcgcgcgc catctccacc cgcccccgct cctatggcgg tccattgcct cgtgaggaat
     1681 tatggcatga gcaggcttga aatccgcgac gtccgcaaga gctacggcaa ggccgagacg
     1741 ctgaagggca tcgacatctc gctggcggat ggcgaattcc tggtgctgct gggaccttcg
     1801 ggctgcggga agtcgacgct gctcaacatc atcgccgggc tgaccgaagc gaccagcggc
     1861 gatatccgca tcggcgaccg ttcgatcatc ggcgtccacc ccaaggaccg cgacatcgcg
     1921 atggtgttcc agtcctacgc gctctatccg aacctgaccg tgcgccgcaa catcggcttc
     1981 gggctggaga tgcgcaaggt tcccgccgcc gagcgcgaca aggcggtgaa ggaggcggcg
     2041 cgactgttgc agatcgagaa cctgctcgac cgcaagccca gccagctttc tggcggccag
     2101 cgccagcgcg tcgccatcgg cagggcgctg gtgcgcaaac cggaagtttt cctgttcgac
     2161 gaaccgcttt ccaacctcga cgcaaagctg cggctggaaa tgcgcgccga aatcaagcgc
     2221 ctgcaccaga tgctgaagac cacgatcgtc tatgtcactc atgaccagat cgaggcgatg
     2281 acgctggcca cgcgaattgc cgtcatgcat gagggccaga tcgagcagtt gggcacgccg
     2341 gaggaaatct ataacaagcc ggcgacgctt tatgtggccg gtttcgtcgg cgcgccaccg
     2401 atgaacatgc tgaacgtgac gcttgcggaa ggggttttaa ggatcgccgg ctccgatgcg
     2461 gcgttcgccc tgccgtcacg ttttacgccg gacaccatgg ccaatggccg cgaacttgtc
     2521 gccggcatca ggccggaggc tttgcatctc gacggggccg gggcggcgcc attttgcttt
     2581 tcagccacca ttgacggtgc ggaattgacc gggccggaac tggtcgtctc cgccagggta
     2641 ggttcgcaaa aggtgacggc gagcctgccg ccccgatccg ccatcactat aggccaaacg
     2701 cgcaccttcg cgttcgatga agcggcgttg cacctgttcg atcctgcgac cggcaaggca
     2761 ttttgaagcg ccgatccgcg ccgaatcaaa gccacatcgg accaagccaa gcccgtcaag
     2821 cacactggac acgcgacgat ggcgcaccgc ccgaaagcgg gaaggggcga cttcgcttga
     2881 cggatttctc ttgatcattt atgactgacg aaagttgaaa ttcgtcagtc atagcgaacc
     2941 catggccctg ttaaacgaaa ccaccgaagc gagttcatca cggcaggaga atgtcgagcg
     3001 catccttgag agcgccgagc ggcttttccg gcattacggc tatggcaaga cgacggttgc
     3061 cgacattgcc cgcgatctcg gcatgtcgcc ggcgaatgtc tatcgtttct tcgcatcaaa
     3121 ggagcagatc catcaggaaa tctgcgggcg catgcttgcg gcgagcttgc atctggccct
     3181 tgcaaactcg aaacggcaat tgcccgccgc agagcgcctg cgcctttgca ttctcgatca
     3241 gcatcaactg accaaggaga cgatgctgga cgagcagaag gtgcatgaga tggtcatcgt
     3301 cgccatcgag cgcgactggc atgtcattga aaagcacgtc gaccgcctgc atcaggttta
     3361 ttccgacatc atccgcgagg gcatcgaggc tggcgagttt cgccaacagg atctcgacag
     3421 cgccgccaga tgcttcggcg cggcgacggc ggccctttgc cacccgcaaa tggtggctca
     3481 atgccttgcg aaagaaaatc gtgcagcgcc cgatgaactg gtcgatttcg cgatcagggc
     3541 gctgaaatag gcgcggccca ggcccgtcat ccgaccttaa cttcaggagc gtgaacgatg
     3601 ctttcgttca agaccttcag tgattttgcc cggccgctcg ctacgctcag cctgcttggc
     3661 gttcttggcg ccggcgttgc cgcctgcacg caggcaaaga cggagccggt cgtggaggtt
     3721 cgcccggtca aggtcgtgca gatcgccaag gccgacgaca cgcgcgatct cgattattcc
     3781 ggcacggtga aggcgcgcac ggaaatgaac ctcggcttcc gcgttgccgg caagatcgtc
     3841 gagcggctgg tcgatatcgg cgacaaggtg aagcccggcg acatcctcgc ccgcatcgac
     3901 ccaaccgatt acgcgcttgc ccaaaagacc gcgcaggcca accttgccgc cgcgcaaaag
     3961 caggtcgaga cggccggcct gacgcagaag cggtcgcagc aattatttgc caagaagttc
     4021 tcctcgcagg cggaactcga ccaggcgacg ctcggttacc agcaggcaat agcggcgcgc
     4081 gatgcggccg cctccgcgct ccagcaggca agcaaccagg tcgcctatac cgacctgaaa
     4141 gccgacagga acggcatcgt caccgccatc ggcgccgaca gcggacaagt ggtcggcatc
     4201 ggcgcgccgg tcgtcacggt cgccatggac ggcgaaaagg aaatccagat cgcggtgccg
     4261 gaaaccgaca tttcgcattt caaggccggc cagaacgtca aggtcggctt ctggaccgat
     4321 gacgaattgc agctgagcgg caaggtccgc gaggtcgccg gcagcgccga cccgcagtcg
     4381 cgcacctttt cggtgcgtgt cagcctggcc gacgatccgc gcgttcttct cggcatgacg
     4441 gccaccgtag aggcgaaggt cgaaaccaag cgaccgttgg aggtcatccc gctggaggcc
     4501 ctggccaagc tggacggcaa gtcgatcgtc tgggtggtgg accatgacac ttcgacggtc
     4561 catgcgcgcg aaatcggcgt cgccgcattc gcggccgatg gcgtgcgcgt cgcgcagggc
     4621 ttgcaggccg gcgatctcgt cgtggccgcc ggcacccagt tcatgagcga gaacctcaag
     4681 gtcaagctgc cggacgccgt tgccgcacag ggcgaaaacc tcgacgccgg cgggaaaacc
     4741 gccgccattc gctgaccctt caatcgcccg aacctcaaga agaggcgacc atgacgaatt
     4801 cgaccgacga gaagaagccc ttcaacctgt cgcgctgggc catcggccat cccagcatc
//
LOCUS       DASYCS010000038        11115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_5078976,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000038 DASYCS010000000
VERSION     DASYCS010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 11115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11115
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5078976"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="VGM46_01570"
     CDS             <1..585
                     /locus_tag="VGM46_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEY4191310.1"
                     /translation="DQLDGAYSFEAPSKLVEAEFNNIWAQVNRDLEQAGRTFADEETT
                     EEEARAEYLRLAERRVRLGLVLAEIGEKAGVTVSDDELQRGLFEQVRRFPGDQQQQVF
                     EFYRSNPEAVNTLRAPMFEEKVVDHLLGQVSVTDKKVSKEELMADDDEVETKAKPAKK
                     AAKKTEAKADDEAGADETKKKAAPKKKAAAKDAE"
     gene            733..2985
                     /gene="parC"
                     /locus_tag="VGM46_01575"
     CDS             733..2985
                     /gene="parC"
                     /locus_tag="VGM46_01575"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEY4191311.1"
                     /translation="MGKGLLPPHSGGGDHIEPVDLKKALEERYLAYALSTIMNRALPD
                     VRDGLKPVHRRIMHAMRLLRLNPEQGFAKCARIVGEVMGKFHPHGDQSIYDALVRLAQ
                     SFSMRYPLVDGQGNFGNIDGDNAAAMRYTEARMTEVATLVLEGITEDAVDFRPTYNEE
                     DEEPVVLPGAFPNLLANGSSGIAVGMATSIPPHNAAELCDAALHLIEYPDAPVARLME
                     FVQGPDFPTGGIVVDSRASILDAYETGRGGFRVRAKWEQEDQGRGTWTIVVTEIPYGV
                     QKARLIEKAAELLIARKLPLLEDIRDESAEDVRIVLVPKSRSVDPGLLMESLFKLTEL
                     ESRFPLNMNVLSRGKVPNVLSLRGVLKEWLEHRREVLLRRSRHRLAEIERRLEILAGY
                     LIAYLNIDEVIRIIREEDEPKQVMMARWSLTDTQAEAILNMRLRALRKLEEFEIRKEF
                     DGLSAEKSQIEALLASETKQWATIRWEIAKVREQYGPETEIGRRRTQFADAPEHNLDD
                     IAHAMIEREPVTVVVSQKGWLRAMKGHLSDYSTLTFKEGDSLKIAFHAQTTDKVLLLT
                     TGGKFYTIGADRLPGGRGHGEPVRIIVDMENDQDIVTAFVHEPKRRLLLVSQQGYGFV
                     VPEEEVVANTRKGRQVMNVKAPDEAKMCLFVTGDQVAIVGINRKLLVFALSEIPEMTR
                     GKGVRLQKYKDGGVLDAKTFELEQGLSWQDSADRTFVKTRDELVEWIGARASAGRMVP
                     KGFPRTGKFG"
     gene            3165..4316
                     /locus_tag="VGM46_01580"
     CDS             3165..4316
                     /locus_tag="VGM46_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008875338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY4191312.1"
                     /translation="MAAPLRSAVIPPLSAARWLLVLVFAAGVYFFHGFLVPVLAALVI
                     AFASWPLYRRLLDAVGGSRTIAATLAILFILAFLVVPISLAGSYAIHELREWVTWAIE
                     TNRNGAPTPHWIATLPVIGPWLNGQWMENINHPGGVGELIQLVSGANIGSIYRGLLAA
                     GGSAFGLLLTMLFMLIALFFAYRDGEAFAAQVDRLGERILPLRWERISRIVPATISST
                     VTGMTIIAIGEGLVLGIAYWLAGVPSPVTLGALTGIMALIPGGAPLSFTLVSIYLAAS
                     GSMFAGAALFIWGAVELFIVDKTLRPKLVGGPIKLPFLPTFFGLIGGVKTMGFLGLFI
                     GPVLMALLVAIWREWLHETDPQEFATSGEVRPLEIEAVPEAAPDRVQAG"
     gene            complement(4304..5191)
                     /locus_tag="VGM46_01585"
     CDS             complement(4304..5191)
                     /locus_tag="VGM46_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009450182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY4191313.1"
                     /translation="MPASANHSKGLLIAAAGGMALTIDIPLIRLAQGETWSILMVRSG
                     TTLLMALLVWAVWRRTAPATVPRLIPGSIGLLVSALYGLGAVTFVLGVFHTLTANLVF
                     ILAFNPMFTALLSWIFLKERPGPATFATMAAMVLGVGIIVGGSLGSGHLFGDLMALST
                     ALLLSSAITITRTSGKDMGFAALMGVIMPCIVAMFVVWHTGYRIESVGWAILDGAVIM
                     PISFFCLATAPKYIAGPEVAMFYLLETVLAPVWVWLVFSEVPSRNSLIGGVVLIIALV
                     FHSTWQLRQGRRRMAPHPA"
     gene            complement(5247..6002)
                     /locus_tag="VGM46_01590"
     CDS             complement(5247..6002)
                     /locus_tag="VGM46_01590"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012092346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HEY4191314.1"
                     /translation="MTQHPTQSPQFFLTAPSPCPYLEGQLERKVFTHLVGDRAPEMND
                     LLTQGGFRRSQNIAYRPACETCRACVSVRILTHEFSPSRNMKRVMQTNGDLVGAMHDA
                     EPSTEQYSLFRTYLDARHRRGGMSDMTVLDYAMMVEDTHVDTKVIEYRRRGPDTFITG
                     KGQGELIAVALTDQMADGLSMVYSYFDPELADRSLGTFMILDHIARAKAMGLPHVYLG
                     YWVNGSRKMNYKMRFMPQEHLGPKGWERYIEGT"
     gene            complement(6141..6611)
                     /locus_tag="VGM46_01595"
     CDS             complement(6141..6611)
                     /locus_tag="VGM46_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010915564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEY4191315.1"
                     /translation="MNARVLDGEILTTGFDDAWLYNGVRSRRIFAVVLDYLIVAALTI
                     PVGILVFLLGILTLGLGWMLFSILVPAVAILYVWNTLGGSGQATVGMKAMGIRLDRLD
                     GRPIDGMTAVVHTVLFWAGNFILTPLVLLVTLFSDRKRTLHDLLLGTVVTRTYR"
     gene            6799..8190
                     /gene="sthA"
                     /locus_tag="VGM46_01600"
     CDS             6799..8190
                     /gene="sthA"
                     /locus_tag="VGM46_01600"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HEY4191316.1"
                     /translation="MQNYDMVVIGSGPSGRRAAVQSAKLGKSVLVIDRGSRLGGVSVH
                     TGTIPSKTLRETVLNLSGWRERGFYGRGYRVKQDISIGDLIQRLHKTLDHEVEVLQHQ
                     FMRNNVRSTRAVAKFLTANSLSLTDDNGETSEIGFDNALISVGTRPHRPDSVPFDKQH
                     VFDSDEMLGLDRLPRTLTVIGGGVIGVEYATIFSALDVPVTLVEPRHSILDFIDREIV
                     DDFIHDMRDRGMSIRLGSAVKSIEVKQNGAEVTLDDGRLIKSEIVLYAAGRTGNVGTL
                     GLHAVGIDVDSRGRIKVDHKTFQTSVPNIYAAGDVIGFPSLASTAMEQGRVAACHAFG
                     VALPPSPETFPYGIYAVPEISTVGQSEEEVRQSGVAYEVGVARFRETSRGHIMGVNSG
                     FLKLIFALDTRRLLGAHIVGEGATELIHIGQAVINLGGTVDFFVNNTFNYPTLAEAYK
                     IAGLDAWNRMGHG"
     gene            8266..8499
                     /locus_tag="VGM46_01605"
     CDS             8266..8499
                     /locus_tag="VGM46_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006201925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF982 domain-containing protein"
                     /protein_id="HEY4191317.1"
                     /translation="MAVKSDTAGTILLLRSAKEASDFLLNSWPGKRSPKHRAALQACH
                     DAMAGDKPAMNARRAFIAAAREVDVFVNDKTAA"
     gene            complement(8560..8706)
                     /locus_tag="VGM46_01610"
     CDS             complement(8560..8706)
                     /locus_tag="VGM46_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191318.1"
                     /translation="MARPPNYDQERKERDRQKAAKKAEKLAAKAAAKERDKPDNGTQD
                     NNEE"
     gene            complement(8802..9176)
                     /locus_tag="VGM46_01615"
     CDS             complement(8802..9176)
                     /locus_tag="VGM46_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006332540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HEY4191319.1"
                     /translation="MSLSEQTATPFGRSMVLAGGLCGAAGVAFSAAATHRGGAFTGTA
                     ASFLLMHAPVFLLIGLAGGNRVLRIGGVILLVGLVVFSGDLLARDYLGSRLFPFAAPT
                     GGTLLLAGWLAIALSALSRQRR"
     gene            complement(9176..10429)
                     /locus_tag="VGM46_01620"
     CDS             complement(9176..10429)
                     /locus_tag="VGM46_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008875349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4191320.1"
                     /translation="MSAGSDTANEAGNPHWRRNLFVSLAGSFTIIVAMTLLLPFLPLY
                     VEELGVKGHAAIVQWSGIAYGATFFAAALVAPLWGRLGDRYGRKAMLVRASFGMAVAM
                     SLTGMAQNIWQLVLLRVLIGFAGGYSSGSTILVAMQTPKDRSGWALGTLAAGIMAGNL
                     VGPLVGGALPPLIGIRNTFLLAGAVIFVSFLATTFLIKEDPRRAGAASGVAGKKQPGG
                     WSSIPDKGPVAAMLFTGMLLMFATMSIEPIITVYVAQLIEDQSRVTLISGVVMSAAAL
                     GSILSASRLGKLADRVGHWNVITGALAASALLLIPQAFVTQGWQLVALRFLMGLALGG
                     LLPCITAVIRHNVPDGVGGNVLGLSISAQYVGQVAGPLLGGFVGGHFGMRSVFLFTCV
                     LMAFGAAYNRFIQVRRARIATAGQH"
     gene            10648..>11115
                     /locus_tag="VGM46_01625"
     CDS             10648..>11115
                     /locus_tag="VGM46_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY4191321.1"
                     /translation="MSQMQSVEKQLREMILALEIGPGERLTERWIESVFNASRTPVRA
                     ALLRLETEGLICREGRGWTVAPINLAELEQVSVYREAVEIAAVRLSAVAGDAGGVEVI
                     EAMLDSCDAETPREEWHRVGMDFHIELARLSGNEFLFRAVRDAMTRLSRARWLE"
BASE COUNT         2166 a         3456 c         3550 g         1943 t
ORIGIN      
        1 gaccagcttg acggcgccta ttcgttcgaa gcgccctcga aacttgtcga agccgagttc
       61 aacaacatct gggcgcaggt caaccgcgat ctggagcagg ccggccgcac ctttgccgac
      121 gaggaaacga cggaagaaga agcccgcgcc gaatatctgc gcctcgccga gcgccgcgtg
      181 cgcctcggcc tggttctggc ggagatcggc gagaaggccg gcgtcaccgt ttccgacgat
      241 gaattgcagc gcggcctgtt cgagcaggtg cgccgcttcc ccggcgacca gcagcagcag
      301 gtgttcgagt tctaccgcag caacccggaa gccgtgaata cgctgcgcgc gccgatgttc
      361 gaggaaaagg tggtcgacca cctgctcggc caggtctccg tcaccgacaa aaaggtgtcg
      421 aaggaagagc tgatggctga cgacgatgaa gtcgagacca aggccaagcc ggccaagaaa
      481 gccgccaaga agaccgaagc caaggccgac gacgaagccg gcgccgacga gacgaaaaag
      541 aaggctgcgc cgaagaagaa ggctgccgcc aaggacgccg aataagttta cggcatcagc
      601 gggcgcaagc cgcgccgttt tttgactgta cttgaaaccc cgcctttccg gcggggtttt
      661 gtttttatca gttcggttgc tgcgccggca tcgcctcttg ccgcgaatca gccgctcggt
      721 taggtcaaac ccatgggaaa agggcttttg ccgccgcaca gcggcggtgg cgatcatatt
      781 gagccggtcg atctgaagaa ggcgctcgaa gagcgctatc tggcctatgc gctgtcgacc
      841 atcatgaacc gggcgctgcc cgatgtgcgc gacgggctga agccggtgca ccgccgcatc
      901 atgcacgcca tgcggctgct gcggctcaac cccgagcagg gcttcgccaa atgcgcgcgc
      961 atcgtcggcg aagtgatggg caagttccat ccgcatggcg accagtccat ctacgacgcg
     1021 ctggtgcgtc tggcgcagag tttctcaatg cgctacccgc tggtcgacgg gcagggcaat
     1081 ttcggcaata tcgacggcga taacgccgcc gccatgcgct acaccgaagc gcgcatgacc
     1141 gaagtggcga cgctggtgct cgaaggcatc accgaggacg ccgtcgattt ccgcccgacc
     1201 tacaacgagg aagacgagga accggtcgtc ctgccgggcg cctttccaaa cctgctcgcc
     1261 aacggctcgt ccggcatcgc ggtaggcatg gcgacgtcga taccgccgca taatgccgcc
     1321 gagctttgcg acgccgcgct gcatctgatc gagtacccgg atgcgccggt ggccaggctg
     1381 atggagttcg tgcaggggcc ggacttcccg accggcggca tcgtcgtcga tagccgcgcc
     1441 tccatcctcg acgcctatga gacgggacgc ggcgggtttc gcgtccgcgc aaagtgggag
     1501 caggaggacc agggccgggg cacctggacc atcgtcgtca ccgagatacc ctatggcgtg
     1561 cagaaggcgc ggctgatcga gaaggcggcc gagcttctga tcgcgcgcaa gctgccgctg
     1621 ctggaagaca ttcgcgacga aagcgccgag gatgtccgca tcgtgctggt gcccaaaagc
     1681 cgctccgtcg atccgggcct gctgatggaa tcgttgttca agctgaccga acttgaaagc
     1741 cgcttcccgc tcaacatgaa cgtgctgtcg cgcggcaagg tgccgaacgt gctgtcgctg
     1801 cggggcgtgc tcaaggaatg gctggagcac cgccgcgagg tgctgctgcg ccgctcgcgc
     1861 caccggcttg ccgagatcga gcggcggctg gaaatcctgg ccggctatct catcgcttat
     1921 ctcaacatcg acgaggtgat ccgcatcatc cgcgaagagg acgagccgaa gcaagtcatg
     1981 atggcccgct ggtcgcttac cgacacgcag gcggaagcca tcttgaacat gcgcctgcgc
     2041 gcgctgcgca agctggaaga attcgaaatc cgcaaggaat tcgacgggct gtcggccgaa
     2101 aaaagccaga tcgaggcgct gcttgcgtcc gagacgaagc agtgggcgac gatccgctgg
     2161 gaaatcgcca aggtccgcga gcaatacggc cccgaaaccg agatcggccg ccgccgcacg
     2221 caattcgccg acgcgccgga gcataatctg gacgacatcg cgcatgcgat gatcgagcgc
     2281 gagccggtta cggtcgtcgt ctcgcaaaag ggctggctgc gggccatgaa ggggcacctg
     2341 tcggattatt cgacgctcac cttcaaggag ggcgacagcc tgaagatcgc cttccatgcg
     2401 cagaccaccg acaaggtgct gctgctgacc accggcggca agttctacac catcggcgcg
     2461 gaccggcttc cgggcgggcg cggccatggc gaaccggtcc gcatcatcgt cgatatggaa
     2521 aacgaccagg atatcgtcac cgccttcgtg catgaaccga agcgcaggct gctgctggta
     2581 tcgcagcagg gctatggctt cgtcgtgccg gaggaggaag tcgtcgccaa cacccgcaag
     2641 ggccggcagg tgatgaacgt caaggcgccg gacgaggcca agatgtgcct gttcgtcacg
     2701 ggcgatcagg tcgccatcgt cggcatcaat cgcaagctgc tggtctttgc gctttcggaa
     2761 attccggaaa tgacgcgcgg caagggcgtg cggctacaga aatacaagga tggcggcgtg
     2821 ctggacgcca agacctttga actggaacaa ggcctgtcgt ggcaggactc ggctgatcgc
     2881 accttcgtca agaccaggga cgaactggtc gaatggatcg gcgcacgcgc ctcggccggg
     2941 cgcatggtgc cgaaaggctt cccgcgcacc ggcaagttcg gctgaggcct cgattggcgg
     3001 ttgccgtcgg ccgtttggca tccagtcacg tttgttgcac tattcgccga tagtgtaggc
     3061 aaaggcggct ttcgaagatc ggagccggcg tcgacaatcg gggtgcaagg cgtttgaagg
     3121 aacccgtaac cagcaggccg cccaagcaac agcgcctttt cggcgtggcg gcgcccttgc
     3181 gttcggcggt cattccgccg ctgtcggccg cgcgctggct gctggtgctg gtgtttgcgg
     3241 cgggcgtcta cttcttccac ggctttctgg tgccggtgct ggcggcgctg gtcatcgcct
     3301 ttgccagttg gccgctttat cggcgtctgc tcgacgccgt cggcggcagc cgcacgattg
     3361 cggcgacgct ggccatcctg ttcatcctgg cattcctggt cgtgccgatt tcgctggccg
     3421 gcagctatgc catccacgag ctgcgcgagt gggtgacatg ggcgatcgag accaaccgca
     3481 acggcgcgcc caccccgcac tggatcgcca ccttgccggt tatcggtcca tggctgaacg
     3541 gccagtggat ggaaaacatc aaccatccag gcggcgtcgg cgaactcatc caactagtca
     3601 gcggcgccaa tatcggcagc atctaccgtg ggctgctggc cgccggcggc tctgccttcg
     3661 gcctgctgct gaccatgctt ttcatgctga tcgccctgtt cttcgcctat cgcgacggcg
     3721 aagcgttcgc cgcgcaggtg gatcgccttg gcgagcgcat cctgccgctg cgctgggaga
     3781 ggatttcacg catcgtcccg gcgacgattt catcgacggt gacgggcatg accatcatcg
     3841 ccatcggcga gggcctggtt cttggcatcg cctattggct ggccggcgtg ccgtcgccgg
     3901 tcacgcttgg cgctctcacc ggcatcatgg cgctcatacc gggcggcgcg ccgctctcct
     3961 tcacgctggt gtcgatctac ctggccgcca gcggctcgat gttcgcgggt gcggcgcttt
     4021 tcatctgggg tgcggtcgaa cttttcatcg tcgacaagac gctgcgtcca aaactggtcg
     4081 gcgggcctat caagctgccg ttcctgccga ccttcttcgg gcttatcggc ggcgtcaaga
     4141 cgatgggttt tctcggcctg ttcatcggcc cggtgctgat ggcgctgctg gtggctatct
     4201 ggcgcgaatg gctgcacgag acggacccgc aggagttcgc cacatcgggc gaagtgcggc
     4261 cgctggagat cgaagccgtt cccgaggccg cgcccgacag ggttcaggcc gggtgagggg
     4321 ccatccggcg tctgccctgg cgcagctgcc acgtcgaatg aaagaccagc gcgataatga
     4381 ggacaacgcc gccgatcagg ctgttgcgtg acggcacttc tgaaaagacc agccacaccc
     4441 ataccggcgc aagcacggtt tccaacaggt agaacatcgc cacctccggc ccggcgatat
     4501 atttcggcgc ggtggcgagg cagaaaaacg aaatcggcat gatgacggcg ccgtcgagga
     4561 tcgcccatcc gaccgactcg atccggtagc ccgtatgcca gacgacgaac atggcgacga
     4621 tgcagggcat gatcaccccc atgagggcgg caaagcccat gtccttgccg ctggttcgcg
     4681 ttatcgtgat ggcgctcgaa agcaaaagcg ccgtcgataa cgccatcagg tcgccgaaga
     4741 gatggccgct gccgagcgaa ccgccgacga tgatgccgac gcccagcacc atcgccgcca
     4801 ttgtcgcaaa ggtggccggg ccgggccgct ccttcaggaa aatccaggat agaagcgcgg
     4861 tgaacatcgg gttgaaggcc agaatgaaaa ccagattggc cgtcagcgta tggaagacgc
     4921 ccagcacgaa cgtcaccgcg ccaaggccgt agagcgctga aaccagaagg ccgatgctgc
     4981 cgggaatgag gcgcgggacc gtcgccggcg cggttcgccg ccacaccgcc cagacaagca
     5041 gcgccatcag cagcgtcgtt cccgaacgca ccatcaggat cgaccaggtt tcgccctgcg
     5101 ccagccggat cagcggaatg tcgatggtaa gggccatgcc gccggccgcc gcgatcaaca
     5161 ggcctttgga atggttggcg ctggcaggca ttgcgcttcg atgctccgtt gcatggtcca
     5221 acgcagcatg gaaggcggcc ggttgttcag gtgccttcta tgtaccgctc ccagcccttc
     5281 gggccaaggt gctcctgagg catgaagcgc atcttataat tcatcttgcg cgagccgttg
     5341 acccagtagc cgagatagac gtgcggcaag cccatcgcct tggcgcgggc gatgtggtcg
     5401 aggatcatga aggtgcccag cgagcggtcg gcaagctcgg ggtcgaaata ggaatagacc
     5461 atcgacagcc cgtccgccat ctggtcggtc aacgccaccg caatcagttc gccctggccc
     5521 ttgccggtga tgaaggtgtc cggcccgcgc cgccggtatt ctatcacctt ggtgtcgaca
     5581 tgcgtgtcct ccaccatcat ggcatagtcg agcacggtca tgtccgacat gccgccgcgg
     5641 cggtggcggg catcgagata ggtgcgaaac agcgaatatt gctcggtcga cggctccgcg
     5701 tcatgcatgg cgccgacgag gtcgccattg gtctgcatga cgcgcttcat attgcggctg
     5761 ggcgaaaatt catgggtgag gatgcgcacg gagacgcagg cgcggcaggt ctcgcaggcc
     5821 ggccggtagg cgatgttctg cgaccggcgg aaaccgcctt gcgtcaggag gtcgttcatc
     5881 tccggcgccc tgtcgccgac aaggtgcgtg aacaccttgc gctccaactg cccctccaga
     5941 taggggcaag gtgaaggcgc cgtcaggaag aactgtggcg actgggtcgg atgttgcgtc
     6001 atcgcgctaa gaaaacactc ccacgaatcg ctctaccttt ggcgcgagcg gcaaaaaatt
     6061 caacagtatt atgcggttcg cacccggcga acgcccccat tttgcgtcca attgctggga
     6121 aacaggcgca aaaggcgtat tcagcgatag gtgcgggtca ccaccgtgcc cagcagcaga
     6181 tcgtgcaagg tgcgcttgcg gtcggaaaaa agcgtgacca gaagcaccag cggcgtcaag
     6241 atgaaattgc cggcccagaa caatacggtg tgcaccacgg cggtcattcc gtcgatcggc
     6301 ctgccgtcaa gcctgtcgag ccggatgccc atcgccttca tgccaacggt cgcctggccc
     6361 gaaccgccaa gcgtgttcca gacatagaga atggcgacgg ccggcacgag gatggaaaac
     6421 agcatccagc caagcccgag cgtcaggatg ccgagcagga aaacgaggat gccgaccgga
     6481 atggtaagcg ccgccacgat caggtaatcg agaaccaccg cgaagatgcg ccgcgagcgc
     6541 acgccattat acagccaggc gtcgtcgaac ccggtcgtca ggatttcacc gtccagaacg
     6601 cgtgcgttca tgtcattcct ctgttttatg tccgctgcat tgttggacat catttcatga
     6661 aatggtgcgg agcagccgcg gattcaagat ttcacgaagc ggtccggttc gactggttga
     6721 attcacttta tgcgtagcgc agcatgcgga caggtctggg tgcaggcgaa tccgatacgt
     6781 caggggacaa gtagttttat gcagaattac gatatggtgg tgatcggcag cggtccgtcc
     6841 ggacgccgcg ccgccgtaca atccgccaag cttggcaaat cggttctggt aatcgacagg
     6901 ggcagcaggc tgggcggcgt ctccgtccat accggcacta ttccgtcgaa gacgctgcgc
     6961 gaaacggtgc ttaatctttc cggctggcgc gagcgcggtt tttacgggcg cggctaccgc
     7021 gtcaagcagg acatctccat cggcgatctc atccagcgct tgcacaagac gctcgatcac
     7081 gaggttgagg tgttgcagca ccagttcatg cgcaacaatg tgcgcagcac gcgcgccgtc
     7141 gccaagttcc ttaccgccaa cagcctcagc ctgacggacg acaatggaga aaccagcgaa
     7201 atcggtttcg acaatgcgct gatttccgtg ggtaccaggc cgcaccgccc cgacagcgtg
     7261 ccgttcgaca agcagcatgt attcgacagc gacgagatgc tgggtctcga ccggctgccg
     7321 cgcacgctga cggtcattgg cggcggcgtc atcggcgtcg aatatgcgac catattttcg
     7381 gcgctcgatg tgccggtgac gctggtcgag ccgcgtcatt ccatcctgga cttcatcgac
     7441 cgcgagatcg tcgatgattt catccacgac atgcgcgaca ggggcatgtc gattcgcctc
     7501 ggcagcgcgg tgaaaagcat cgaggtcaag cagaacggcg ccgaggtcac actggacgat
     7561 ggccggctga tcaagtcgga gatcgtgctt tacgctgccg gccgtactgg caatgtcgga
     7621 acgctgggcc tgcacgcggt cgggatcgac gtcgattcgc gcggccgcat caaggtcgat
     7681 cacaagacat tccagacttc ggtgccgaac atctatgccg ccggcgatgt cattggtttc
     7741 ccgagcctcg cctcgaccgc catggagcag ggcagggttg ccgcctgtca cgccttcggc
     7801 gttgccttgc cgccgtcacc ggagaccttt ccctatggca tttacgcggt gccggagatt
     7861 tccacggtcg gccagtctga ggaagaggtg cgccagagcg gcgtcgccta tgaggttggc
     7921 gtggcgcggt ttcgcgagac atcgcggggc cacatcatgg gcgtcaattc gggcttcctg
     7981 aagctgatct tcgcgctcga cacccgccgc ctgcttggcg cgcatatcgt cggcgaaggc
     8041 gcgaccgaac tgatccacat cggccaggcg gttatcaacc tgggcggcac ggtggacttt
     8101 ttcgtcaaca ataccttcaa ttacccgacg cttgccgaag cctacaagat cgccggcctc
     8161 gatgcctgga accggatggg ccacggctag ggaattttca ccctcgctta ttgcgcatca
     8221 tgggcgtaag agcacgccat ggaaaacaat cggtttgata cacccgtggc cgtcaagtcg
     8281 gacacggcgg gaacgatttt gctgttgcga tcggcgaagg aagcttcgga ttttcttttg
     8341 aacagctggc cgggcaagcg cagccccaag caccgggcgg cattgcaggc ctgccacgac
     8401 gccatggccg gcgataagcc ggcgatgaac gccagacgcg cctttatcgc ggcggcgcgg
     8461 gaagtcgatg tcttcgtgaa cgacaaaacc gccgcttgag catcggccgc cgtatttcaa
     8521 tgcaggggcg ttcgacgccg taagaatagc gccttggcgc tattcttcgt tgttgtcctg
     8581 cgtgccgttg tcgggcttgt cacgttcctt ggcggcagcc ttggctgcca gcttttcagc
     8641 cttcttggcc gccttctgcc ggtcgcgttc cttacgctcc tgatcgtagt tcggtggtcg
     8701 cgccatatcg attcctttct tcgcctaagc tcttgcgtag ggctatcgac acgcaaatac
     8761 aacagcgcag ccggccaaaa ggcgcgggcg gcttcagcct gtcaccgcct ttgccgcgac
     8821 agcgccgata aggcgatcgc cagccatccg gccagcagca gcgtgccgcc ggtcggggcc
     8881 gcgaacggaa aaagccgcga gccgagatag tcgcgggcaa gcaggtcgcc gctgaacacc
     8941 accaggccga caagcaggat cacgccgccg atacgcagca cgcgattgcc gccagccagg
     9001 ccgataagca ggaacacggg cgcatgcatc agcaggaacg acgccgccgt tccggtgaat
     9061 gcgccgccgc gatgcgtggc cgccgccgaa aaggcgacgc ctgccgcgcc gcataacccg
     9121 ccggccagga ccatcgagcg gccaaatggg gtggcggtct gttcgctcaa actcatcaat
     9181 gttgtcctgc tgttgcaatg cgcgcacggc gcacctgtat aaaccggtta tacgctgcgc
     9241 cgaaggccat caacacgcag gtgaacagga aaaccgaacg cattccaaaa tgcccgccga
     9301 caaagccgcc aagcagcggg ccggccacct gtccgacata ttgagccgag atggaaaggc
     9361 caagcacatt gccgccgacg ccgtccggca cattgtggcg gatgacggcg gtgatgcagg
     9421 gcaacaggcc gccgagcgcc agccccatca ggaagcgcag cgccaccaac tgccagcctt
     9481 gcgtgacgaa ggcctgcggt atcagcagca gcgccgacgc cgccagcgcc ccggtaatca
     9541 cgttccagtg cccgacccgg tcggcgagct tgccgaggcg cgaggcggaa aggatgctgc
     9601 caagcgccgc agccgacatg accacgccgg aaatcagcgt cacgcggctt tggtcctcga
     9661 tcagttgcgc gacatagacg gtaatgatcg gctcgatcga catggtggcg aacatcaaca
     9721 gcatgccggt gaacagcatg gcggccaccg ggcctttgtc gggaatgctc gaccatccgc
     9781 ccggctgctt ttttccggca acgccggagg ccgcgcccgc gcgccgggga tcttccttga
     9841 taaggaaggt ggtggcgagg aaggaaacga aaatgaccgc gcccgccaaa aggaaggtgt
     9901 tgcggatgcc gatcagcggc ggcaacgcgc cgcccaccag cgggccgacc agattgcccg
     9961 ccatgatgcc ggcggcgagc gtgcccagcg cccagccgga gcggtctttc ggcgtctgca
    10021 tggcgaccag aatggtcgag ccggacgaat agccgccggc aaagccgatc agcacgcgca
    10081 gcagcaccaa ttgccagatg ttctgggcca tgccggtcag cgacatggcg accgccatgc
    10141 cgaagctggc gcgcaccagc atcgccttgc gaccgtagcg gtcgcccagc cgcccccata
    10201 gcggcgccac cagcgcggcc gcgaagaagg tggcgccata ggcgatgccc gaccattgca
    10261 cgatggccgc atgccctttg acgcccagct cttccacata gagcggcagg aacggcagca
    10321 gcagcgtcat cgccacgatg atcgtgaagg aaccagccag gctgacgaac aggtttcgcc
    10381 gccagtgcgg gtttcccgcc tcatttgccg tgtcgctacc tgccgacatt ttcttgcttg
    10441 tttcctgttg ggtggatatg agtggcattg gataccggtt tgggatacca agctggtatc
    10501 cgtaatggac gccgaccggc gcctttgtca atccgcttgt tggccaatca tcggtagcca
    10561 ttagccagtc aatgcaaaca tcaggccatg gccgggccgg cacatctgaa gctggtccag
    10621 ggcaaagcga accgggcgag gaataacatg tcgcagatgc agagcgtcga gaagcaattg
    10681 cgggaaatga tcctcgcctt ggagatcggc cccggcgagc gcctcaccga gcgctggatc
    10741 gaaagcgtct tcaacgcatc gcgcacccct gtccgggcgg cgcttctacg gctggaaacg
    10801 gaagggctga tctgccgtga agggcgcggc tggaccgtcg cgcccatcaa ccttgccgag
    10861 cttgagcagg tttcggtcta tcgtgaggcc gtggagatcg cggcggtgcg gctaagcgcc
    10921 gtggcgggtg acgctggcgg cgtggaagtc atcgaagcga tgctcgattc ctgcgatgcc
    10981 gaaacgccgc gcgaggaatg gcaccgcgtc ggcatggatt tccatatcga gctcgcacgg
    11041 ctttccggca acgaattcct ttttcgggcg gtgcgcgacg ccatgacccg gctttcgcgc
    11101 gcgcgctggc tcgaa
//
LOCUS       DASYCS010000039         4361 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1455759,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000039 DASYCS010000000
VERSION     DASYCS010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1455759"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2292)
                     /gene="alaS"
                     /locus_tag="VGM46_01630"
     CDS             complement(<1..2292)
                     /gene="alaS"
                     /locus_tag="VGM46_01630"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008934152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEY4191322.1"
                     /translation="MSGVNEIRSAFLDYFRKEGHEVVASSPLVPRNDPTLMFTNAGMV
                     QFKNVFTGLEHRPYLRATTSQKCVRAGGKHNDLDNVGYTARHHTFFEMLGNFSFGDYF
                     KERAIELAWNLITREFGLAKDRLLVTVYHTDDAAAEFWKKIAGLPEEKIIRIATSDNF
                     WAMGDTGPCGPCSEIFYDHGDHIPGGPPGSPDQDGDRFIEIWNLVFMQFAQLTKDERI
                     DLPRPSIDTGMGLERIAAVMQGVHDNYDIDLFKALIRASEEATGVKAEGANRASHRVI
                     ADHLRATSFLIADGVLPSNEGRGYVLRRIMRRAMRHAQLLGAADPLMWRLVPALVREM
                     GQAYPELVRGEALITETLKLEETRFRKTLARGLNLLSDATESLSSGGRLDGETAFKLY
                     DTYGFPLDLTQDALRQRGVSVDLEGFNAAMARQKAEARANWAGSGDSATEAVWFGVRE
                     KAGATEFLGYATEQAEGVIQALVRDGATIDSAASGDPVAVVVNQTPFYGESGGQMGDT
                     GTITGEGFVIEVSDTQKKGDGLFVHMGKVASGTVGVGAAVELKVDHARRSLLRANHSA
                     THLVHEALREVLGSHVAQKGSLVAPERLRFDFSHPKPISADELEAVEAMANAIVIQNS
                     PVTTRLMSIDDAIAEGAMALFGEKYGDEVRAVSMGTAIAGAKAGKPYSVELCGGTHVG
                     ATGEIGLIRLVSEGAVAAGVRRIEAVTGEAARKHLDEQDKRLKSVASSLKVAPADVPA
                     RIDALLDERKRLERELNEARKKLA"
     gene            complement(2543..3643)
                     /gene="recA"
                     /locus_tag="VGM46_01635"
     CDS             complement(2543..3643)
                     /gene="recA"
                     /locus_tag="VGM46_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008875470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEY4191323.1"
                     /translation="MAQNSLRLVEDKAVDKSKALDAALSQIERAFGKGSIMRLGANEQ
                     VVEIETVPTGSLGLDIALGVGGLPRGRIIEIYGPESSGKTTMALHTVAEAQKKGGICA
                     FVDAEHALDPVYARKLGVDLENLLISQPDTGEQALEICDTLVRSGAIDVLVVDSVAAL
                     TPRAEIEGEMGDSLPGLQARLMSQALRKLTASISRSNTMVIFINQIRMKIGVMFGSPE
                     TTTGGNALKFYASVRLDIRRIGSVKDRDEVVGNQTRVKVVKNKLAPPFKVVEFDIMYG
                     EGVSKTGELIDLGVKAGIVEKSGAWFSYNSQRLGQGRENAKLFLRDNPDMAREVEMAI
                     RQNAGLIAEKFLENGGPEKDDGEDFEGEAGAM"
     gene            complement(4242..>4361)
                     /locus_tag="VGM46_01640"
     CDS             complement(4242..>4361)
                     /locus_tag="VGM46_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HEY4191324.1"
                     /translation="TGFTFSNPNQSSACGCGESVELKPADLKALAEARAQSAA"
BASE COUNT          825 a         1409 c         1298 g          829 t
ORIGIN      
        1 cgccagcttc ttgcgagcct cattcagttc ccgctcaagc ctcttgcgct cgtcaagcaa
       61 tgcgtcgatg cgcgccggaa cgtcggcagg cgcgaccttc agcgacgagg ccaccgattt
      121 cagtcgcttg tcctgctcat cgaggtgctt tcgcgccgcc tcgccagtca ccgcctcgat
      181 gcggcgtacg ccggcagcga ccgccccttc cgacaccagc cggatcagcc cgatctcgcc
      241 tgtcgcgccg acatgggtgc cgccgcacag ctcgaccgaa taaggcttgc ccgcctttgc
      301 gcccgcgatg gccgtgccca tggaaaccgc acgaacctcg tcgccatact tttcgccgaa
      361 cagcgccatg gcgccttcgg caatggcatc gtcaatgctc atcaggcgcg tggtgaccgg
      421 gctgttctgg atgacgatgg cattggccat cgcttcgacg gcttccagtt cgtcggcgga
      481 aatcggcttt gggtgcgaaa agtcgaaacg caaccgctcg ggtgcgacca gcgaaccctt
      541 ttgcgcgaca tggctgccca gcacctcgcg caacgcctcg tgtacaagat gggtcgcgga
      601 atgattggcg cgcagcagcg agcggcgggc atgatcgacc ttcagttcca cggcggcgcc
      661 aaccccgacc gtgcccgagg caaccttgcc catatgcacg aacaggccat cgcccttctt
      721 ctgcgtatcg ctgacctcga tgacgaagcc ttcgccggta atcgtgccgg tgtcgcccat
      781 ctggccgccg gactcgccgt aaaacggcgt ctggttgacg accaccgcga ccgggtcgcc
      841 ggaagcggcg ctgtcgatcg tcgcgccgtc cctgaccagg gcctggatga cgccttcggc
      901 ctgctcggtc gcatagccga ggaattccgt cgcgccggcc ttctcgcgaa cgccgaacca
      961 aaccgcctcg gtcgccgagt caccggaacc ggcccaattt gcccgcgcct ccgccttctg
     1021 gcgcgccatg gcggcgttga aaccttcgag atcgaccgac acaccgcgct ggcgcagcgc
     1081 gtcctgcgtc agatcgagcg gaaagccgta ggtgtcataa agcttgaagg ccgtctcgcc
     1141 atcgagccgg ccgccgctgg agagcgattc cgtggcatcc gaaagcaggt tgagaccgcg
     1201 cgcaagcgtc ttgcggaagc gggtttcctc aagcttcagc gtctcggtga tcagcgcctc
     1261 gccgcgcacc agttcgggat aggcctggcc catctcgcgc accagtgctg gaaccagccg
     1321 ccacatcaac ggatcggccg cgcccagcaa ctgggcgtgg cgcatggcac ggcgcatgat
     1381 acggcgcagg acgtatccgc gcccctcatt cgaaggcagg acgccgtcgg cgatgaggaa
     1441 actggtcgcg cgcagatgat cggcgatgac gcggtggctg gcccggttgg cgccctccgc
     1501 cttgacgccg gtggcctcct cggaagcgcg aatcagcgcc ttgaacaggt cgatgtcgta
     1561 attatcatgg acgccctgca tgacggcggc gatgcgctcc aaccccatgc cggtatcgat
     1621 cgaaggacga ggcaggtcga tgcgctcatc cttcgtcaac tgcgcgaact gcatgaagac
     1681 gaggttccaa atctcgatga agcgatcgcc atcctgatcc gggctgccgg gagggccgcc
     1741 tggaatatgg tcgccatggt cgtagaaaat ctccgagcaa ggcccgcacg gaccggtatc
     1801 gcccatcgcc cagaaattat cgctggtggc gatgcggatg atcttctcct ccggcaggcc
     1861 ggcgatcttc ttccagaact cggccgcggc atcgtccgtg tgatagaccg tcaccagcaa
     1921 ccggtccttg gcaaggccga attccctggt gatcaggttc caggcaagct cgatggcgcg
     1981 ctccttgaaa tagtcgccga acgagaaatt gccgagcatc tcgaaaaacg tgtgatggcg
     2041 cgcggtgtag ccgacattgt cgagatcgtt gtgcttgccg ccggcgcgca cgcatttctg
     2101 cgaggtggtg gcgcgcaaat agggccgatg ttccagaccg gtgaagacgt tcttgaactg
     2161 caccatgccg gcattggtga acatcaatgt cgggtcgttg cgcgggacaa gcgggctcga
     2221 tgccacgacc tcgtgacctt ccttgcggaa atagtcgagg aatgccgacc ggatttcgtt
     2281 cacgccactc atgaatgcag cctttttcga gaaaccgccg gaaagcggcg gaaatggaca
     2341 ccggcctttt aacgggcgcg tcccaccctg tccagaaaca caagacagga tcgaaacatg
     2401 ggccgtgccc cctcacgccg cttgaccgga aaacgcaaac cgccggcttg atgccggcgg
     2461 tttgaaatac atggtatacg gttcccagac catccgtccg gcatccccat gcaggcgaac
     2521 gggccgactt ggcgaaccgg ccctacatcg cgccggcctc accttcgaaa tcttcgccgt
     2581 cgtccttttc agggccgccg ttttcaagga atttctcagc aatcaacccg gcattctggc
     2641 ggatcgccat ttccacctcg cgcgccatat cgggattgtc gcgcaggaac agcttggcat
     2701 tctcgcgacc ctggccaagg cgctgcgaat tataggagaa ccaggcaccg gacttttcga
     2761 cgatgccggc cttgacgccg agatcgatca attcgcccgt cttggaaacg ccctcgccat
     2821 acataatgtc gaactcgacc actttgaagg gcggcgccag tttgttcttg accaccttga
     2881 cgcgggtctg gttgccgaca acctcgtcgc gatccttgac cgagccgatg cggcgaatgt
     2941 cgaggcggac cgaggcgtag aatttcagcg cgttgccgcc ggtcgtcgtt tcgggcgagc
     3001 cgaacatgac gccgatcttc atgcggatct ggttgatgaa gatgaccatg gtgttggagc
     3061 gcgagatgga ggcggtgagc ttgcgcagtg cctggctcat caggcgggcc tgcaagccgg
     3121 gcagcgaatc gcccatttcg ccctcgattt cagcgcgcgg cgtcagcgcc gcaaccgaat
     3181 cgaccaccag cacgtcgata gcgccggagc gcaccagcgt gtcgcagatt tccagcgcct
     3241 gctcgccggt gtccggctgc gagatcagca ggttttcaag atcgacgcca agcttgcggg
     3301 cgtagaccgg atcgagcgca tgctcggcat cgacaaacgc gcagatgccg cccttcttct
     3361 gggcctcggc caccgtatgc agggccatcg tcgtcttgcc cgagctttcc gggccgtaaa
     3421 tctcgatgat gcggccgcgc ggcaagccac cgacgccaag cgcaatatcg agaccgagcg
     3481 agcctgtcgg caccgtctcg atctccacca cctgctcatt cgcgccgagc cgcatgatcg
     3541 agcccttgcc gaaagcgcgt tcgatttgcg acagcgccgc atccagagcc tttgatttat
     3601 ccaccgcttt gtcctctaca agccgcaaag aattctgagc catgatacat caccccttga
     3661 tcgtcaatga aagccggacg gcaccgtgaa cctgtcttgt atgtactctt tttgttccct
     3721 gatgacaaga gggaccaagc ccttgtaaag gctgcattat ctttatttgt tccttttttg
     3781 tccaataatt tgtgtgaaac atcctcaggc tccgccgatc ggcctggccc acggcgcgaa
     3841 tcggccgggc cgattgcccg accgccacgg caagccggga ggaccgttca tatctcgtcg
     3901 atacgctcgt tgtccggcga gtgcgagttc tgcaagaagc acggcagtat gctgtggccc
     3961 tacgatgcat cccgaccaaa ccactgcatc ccttcgcctt cgcgatagac gacggccttt
     4021 cggttcgtag gagcgcacgc acccggttct gaaatacaga atgaagctta ggtgcagggc
     4081 gatgctgagg tgcggggcga actgggtcga gccagaaatc cgcaagagtt acgaggcgtt
     4141 cgatttctat ccgcaaacgc cgatcgccgg ctggcaagcg cgtcgggaag caaccggcga
     4201 tttcggtaaa atagccgggc agcgcccggc ttgtcggaaa atcaggccgc gctttgcgcc
     4261 cttgcctcgg ccagcgcctt caggtcggcg ggcttcagtt cgacggattc gccgcagccg
     4321 caagccgaac tctggttcgg attcgagaag gtaaagccgg t
//
LOCUS       DASYCS010000040         7985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3630363,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000040 DASYCS010000000
VERSION     DASYCS010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 7985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7985
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3630363"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VGM46_01645"
     CDS             <1..364
                     /locus_tag="VGM46_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010914492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEY4191325.1"
                     /translation="ETLSKGSSARSVERDLANGVTRYRISEDGGLNRHPGNGLSTRDA
                     HEEIWSITQGDPLSMTAEIRWTCEARREGWRTRTHIVSTMSCTETEWVISERVEAFHE
                     DKSVFARERASRIPRDHM"
     gene            complement(577..652)
                     /locus_tag="VGM46_01650"
     tRNA            complement(577..652)
                     /locus_tag="VGM46_01650"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(617..619),aa:Ala,seq:ggc)
     gene            complement(833..1219)
                     /locus_tag="VGM46_01655"
     CDS             complement(833..1219)
                     /locus_tag="VGM46_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007008520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02300 family protein"
                     /protein_id="HEY4191326.1"
                     /translation="MAKSELGTKRIDPETGRKFYDLNKDPVVSPYTGKTYPRSYFEEG
                     KAAVIEEEEVVAKEADSDEEEGVEVVSLEEADDEAKTSDLPDLDDDEDDVDLGDDEDD
                     TFLADEEEEDDDVGDIIGVGDDDDDA"
     gene            1412..2782
                     /gene="aroA"
                     /locus_tag="VGM46_01660"
     CDS             1412..2782
                     /gene="aroA"
                     /locus_tag="VGM46_01660"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEY4191327.1"
                     /translation="MSHEAAPLPATSRKSPSLSGTVRVPGDKSISHRSFMFGGLASGE
                     TRITGLLEGEDVMRTGAAMKAMGAHIEKHGDEWIIRGTGNGALLQPEGPLDFGNAGTG
                     SRLTMGLVGTYDMETTFIGDASLSGRPMGRVLDPLRQMGVQILQASPGDRMPITLRGP
                     KHASPITYRVPMASAQVKSAVLLAGLNTAGVTTVIEPVMTRDHTEKMLKGFGANLSVE
                     TDERGVRHIFIEGQGKLTGQTIAVPGDPSSAGFPLVAALIVPGSDIIIENVLMNPTRT
                     GLLLTLREMGGDIEILDPRNAGGEDVADLRVRHSDLKGVTVPPERAPSMIDEYPVLAV
                     AACFAEGETLMQGLEELRVKESDRLAAVANGLKLNGVDCTEGETTLAVRGRPGGKGLG
                     GGAGHDAVKTHLDHRIAMSFLVMGLASEKPVTVDDQTMIATSFPEFMGLMRGLGAHID
                     VSKAAA"
     gene            2826..4409
                     /locus_tag="VGM46_01665"
     CDS             2826..4409
                     /locus_tag="VGM46_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006204008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-guanidino-2-oxopentanoate decarboxylase"
                     /protein_id="HEY4191328.1"
                     /translation="MTTVGEALIHLLDAHGVDTVFGIPGVHTVELYRGLAASRIRHVT
                     PRHEQGAGFMADGYARACGRPGVAFVITGPGLTNAITAMGQARADSVPMLVISGVNAT
                     ATLGKGLGFLHELPDQRGMMEKVALFSQRITQAGELPAALARAFSLFATERPGPVHIE
                     IPIDVMAMPADGLAFVPAKTTRPAPDMAKVARAVAMLGAARRPLILAGGGARQASALL
                     RRLGERLGAPVVQTANARGMLYRHPLGVPASPSLKAVRALIAEADAVIAVGTEFGPTD
                     YDMYGDGGFSIPANLIRIDIGAEQLGRRPASVSIHADSGEAVEALLAEIDKASVPANP
                     DGASRAQATRKAALAEIGPALQAQLRAVEIIRDALPGSIIVGDSTQPIYAANLYYDHD
                     RPGGWFNAATGFGALGYGPPAAIGAALAVPGAAVVCLTGDGGFQFTLPELGAALDADA
                     PVIFVVWNNRGYREIETSMRDVGVEPVGVSPAPPDFCKLAEAYGIDAERLSNMDGLAD
                     ALRKARATKRAYVIEISVE"
     gene            4459..5166
                     /locus_tag="VGM46_01670"
     CDS             4459..5166
                     /locus_tag="VGM46_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015314261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY4191329.1"
                     /translation="MTASLQGKHIVITGGTGALGGTVVAKLLSEGAVCHVPNAHATAP
                     AHFSYAGNDRVKLANNVDLADPVKVDAFYARIPELWASIHLAGGYAGAPIEATDPASF
                     DEMMDTNARTTFLCCRAAVRSMLASGVAGRIVNVSARPGLDPRRGAGMVAYAASKAAV
                     AAMTVALAEEVKGNGILVNAIAPSTLDTPANRAAMQKADFTRWVDPEAIAETVAALVS
                     ARNILTSGALIPLYGRA"
     gene            complement(5181..5513)
                     /locus_tag="VGM46_01675"
     CDS             complement(5181..5513)
                     /locus_tag="VGM46_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191330.1"
                     /translation="MKYMMLMVALLGLGGCAGHSLADAEDSVMVVSLVYDKVPCGRLT
                     NERNALAREVGVPTGAKVTFTPVSTGFGVVIPDFRSNERRKRDSAVGKIVGMNDSLLR
                     RCSAPVSN"
     gene            5723..6397
                     /gene="rpe"
                     /locus_tag="VGM46_01680"
     CDS             5723..6397
                     /gene="rpe"
                     /locus_tag="VGM46_01680"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEY4191331.1"
                     /translation="MTAKTLIAPSVLACDFSRLGDEVEAVTQAGADWIHLDVMDGHFV
                     PNITFGPPVIKAIRNRTKAFFDCHLMIEPADPYLAAFAEAGCDGMTVHAEAGPHLDRS
                     LQTIRNLGKKAGVALNPATPESAIEYVLDRLDLILVMTVNPGFGGQAFIAAMVDKVRR
                     VKAMVGDRPIHIQIDGGVSPQTAPLVTAAGANVLVAGAAVFKGDSVDAYRRNISAIRS
                     AADAAI"
     gene            complement(6401..7561)
                     /gene="nagA"
                     /locus_tag="VGM46_01685"
     CDS             complement(6401..7561)
                     /gene="nagA"
                     /locus_tag="VGM46_01685"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEY4191332.1"
                     /translation="MSERFALIGARIFDGDAWHDDAALLVNGGEVEGIVQAASLPAEI
                     EKKETGGGILAPGFVDLQVNGGGGVMLNDHPDVASIEKICRAHAPFGTTALLPTLITD
                     TPEVTAAAIAAGIEAGRQQVPGFLGLHLEGPHLSLARKGAHDPALIRPMTDDDLAALI
                     AGCAHLPVLLTTIAPESVDLQRVSALAQAGVVVSLGHSDVGHAAANAYAEAGASMVTH
                     LFNAMSQIGNREPGLAGAAIDGGSLHAGLIADGIHVDPATMAIALRAKRGPAKVFLVT
                     DAMATIGTDMKSFTLNGRTIHRKDGRLTLADGTLAGADLDMISAVRFVHRAIGLDLGE
                     ALRMASLYPAQAVGQSNRIGRLAKGTAANIVALSSALDVQGVWIDGRPVFAA"
     gene            complement(7558..>7985)
                     /locus_tag="VGM46_01690"
     CDS             complement(7558..>7985)
                     /locus_tag="VGM46_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEY4191333.1"
                     /translation="QDRSLFVLGRGPSFAIASEAALKFKETCGVHAEAYSAAEVMHGP
                     LARVGPGFPVLALAARDASEPSMIEACEGLAGKGASVFVTSAAAGKAHHLPFAATGHP
                     LTDPLALIVSFYAFVEAFARHRGLDPDTPPNLRKVTETR"
BASE COUNT         1456 a         2603 c         2545 g         1381 t
ORIGIN      
        1 cgaaactctt tccaaagggt cgtccgcccg ctccgtcgaa cgtgacctgg cgaacggcgt
       61 aacgcgctac cggatcagcg aggatggcgg cctcaaccgg catccgggca acggtttgag
      121 cacgcgcgac gcccacgagg aaatctggtc gatcacccag ggcgacccgc tttcgatgac
      181 ggccgaaata cgctggacgt gcgaggcgcg gcgcgaaggc tggcgaacgc gcacgcatat
      241 cgtctcgacg atgtcgtgta ccgaaaccga atgggtcatc tcggagcgcg tcgaggcttt
      301 ccacgaagac aaaagcgtgt tcgcccgcga acgcgccagc cgcattccgc gcgaccatat
      361 gtagggcaga ggatagtaat ttttcctgga tgaaaaccgg tcatgacgtg ccgcgtctga
      421 cctgattttt cgactgaggg aagcgtcggc gcgaatggcg gtgcctgggc aggccggtgc
      481 cagcggcgac tacggcaacc accgagacga taacctgctt acccactgaa atgtcgggat
      541 tatttgaacc agccttgaac gccaggcggg gctggttggt ggagccaagc gggatcgaac
      601 cgctgacctc ttgcatgcca tgcaagcgct ctcccagctg agctatggcc ccattccggc
      661 agatgccggc gccgttgccg gcgaaattcg gtgcgggctg aaaaccgtac cgttaagtgc
      721 aggcggcttc taaccccgcc tttcgtcaag atcaagccta ggacgacggc tttttcacgt
      781 tccaggcaaa agcgagcgaa aggcgcgttg ccgcgccggc gcccgccaag gctcaagcgt
      841 cgtcgtcgtc gtcgcccacg ccgataatat cgcctacatc gtcgtcttct tcttcctcgt
      901 cggcaaggaa ggtatcgtct tcgtcgtcgc cgaggtcgac gtcatcttcg tcgtcgtcga
      961 gatcgggcag gtcgctcgtc ttggcctcgt cgtcggcctc ctcaagcgac acgacctcga
     1021 cgccctcttc ctcgtcgctg tcggcttcct tcgcgaccac ttcctcttcc tctatgacgg
     1081 cagctttgcc ttcctcgaaa taggagcgcg gataggtctt ccccgtgtac ggagaaacga
     1141 ccggatcctt gttcagatcg tagaatttcc gacccgtctc ggggtcaatg cgtttggtgc
     1201 ccagttcgga ttttgccact acagacctcg tatggaaaag tctggtcccc ttaaccagag
     1261 tttgcggcgc tgtcaaagcc taatgcccag cctcgaaaaa gctggcttca aacggctttc
     1321 catgcggggg atgaccgttt gcttcgagcg tgatacgaag cggccgcaat aatgaagaac
     1381 gcccgcgcag cgggttttgc aaggatataa gatgtcgcac gaagcagcgc cgctgccggc
     1441 caccagccga aaatcccctt ccctttccgg caccgtgcgg gtgccgggcg acaaatccat
     1501 ctcgcaccgg tcgttcatgt tcggcggcct cgcttccggc gaaacccgca ttaccggcct
     1561 gctcgaaggc gaggacgtga tgcgcacggg cgccgccatg aaggcgatgg gcgcgcatat
     1621 cgaaaaacat ggcgacgagt ggatcatccg cggcaccggc aacggggcgc tgctgcaacc
     1681 ggaaggcccg ctcgatttcg gcaatgccgg caccggttcg cgcctcacca tgggccttgt
     1741 cggaacctac gacatggaaa ccactttcat cggcgacgcc tcgctgtcgg gcaggccgat
     1801 ggggcgcgtg ctcgacccgc tgcgccagat gggcgtccag atattgcagg cgtcgcccgg
     1861 cgaccggatg ccgatcaccc tgcgcgggcc aaaacatgca agcccgatca cctaccgcgt
     1921 gccgatggcc tcggcgcagg tgaagtccgc cgtgcttctg gccgggctga acacggccgg
     1981 cgtcaccacc gtcatcgaac cggtgatgac gcgcgaccat accgaaaaga tgctgaaagg
     2041 gttcggcgcc aatttgtcgg tggaaaccga cgagcgcggc gtgcgccata tattcatcga
     2101 gggccagggc aagcttaccg gccagaccat tgccgtgccg ggcgatccat cttcggccgg
     2161 tttcccgctg gtggcggcgc tgatcgtgcc gggttccgat atcatcatcg aaaacgtctt
     2221 gatgaacccc acccgcaccg ggcttctgct taccttgcgg gaaatgggcg gcgacatcga
     2281 aattcttgat ccgcgcaacg cgggtggcga agacgtcgcc gatcttcgcg tccgccattc
     2341 cgatttgaag ggcgtgacgg tgccgcccga acgcgcgccg tcgatgatcg acgaatatcc
     2401 ggtcctggct gtcgccgcct gctttgcgga aggtgaaacc ctgatgcagg gccttgagga
     2461 actgcgcgtc aaggaaagcg accggctggc ggctgtcgcc aacggcctca agctgaacgg
     2521 cgtcgattgc accgaaggcg agacgacgct tgccgtgcgc ggcaggcccg gtggcaaagg
     2581 cttgggcggc ggtgctgggc acgacgcggt gaagacgcat ctcgaccacc gcatcgccat
     2641 gagcttcctt gtcatgggcc tggccagcga aaagccggtt accgtggacg accagacgat
     2701 gattgccacg agcttcccgg aattcatggg cctgatgcgc gggcttggcg cgcatatcga
     2761 cgtttcgaaa gccgcggcat gaaggcctgc ttgtcctgtc gccggatgcg ggagcggcag
     2821 ccttaatgac cactgtcggc gaagcgctca tccacctgct cgatgcccat ggggtcgata
     2881 ccgtcttcgg cattcccggc gtccatacgg tcgagttgta cagggggctg gcggcttcgc
     2941 gcatacgcca tgtcacgcct cgccatgaac agggcgccgg cttcatggcc gatggctatg
     3001 cccgcgcttg cggccgcccc ggcgtcgcct tcgtcattac cgggccgggg ctgaccaatg
     3061 cgattaccgc catggggcag gcccgcgccg attccgtgcc gatgctggtg atttccggcg
     3121 tcaacgcgac ggcgacgctg ggcaaggggc tgggcttcct gcacgaattg cccgaccagc
     3181 gcggcatgat ggagaaagtg gcgctgtttt cgcagcgcat cacgcaagcc ggcgaactgc
     3241 ccgccgccct tgcccgcgct ttctcgctat ttgcaacgga acggcccggc cccgtccata
     3301 tcgagattcc aatcgacgtg atggccatgc cggccgatgg gctggccttt gtaccggcca
     3361 agacgacacg ccccgctccc gacatggcga aggtcgctcg cgccgtggcg atgctgggcg
     3421 cggcccgtcg cccgctcatc ctggccggtg gcggcgctag gcaggccagc gcgctgttgc
     3481 gacggcttgg cgagcgcctc ggcgcgccgg tggtgcaaac ggccaatgcg cgcggcatgc
     3541 tctaccggca cccgcttggc gttcccgcca gcccgagcct gaaagcagtg cgcgcgctga
     3601 tcgccgaggc cgatgccgtc atcgcggttg gaaccgaatt cggcccgacc gactatgaca
     3661 tgtatggcga tggcggcttt tccataccgg ccaatctcat ccgcatcgat atcggcgccg
     3721 aacagcttgg ccggcgcccg gccagcgttt cgatccatgc cgatagcggc gaggcggttg
     3781 aagcgctgct cgccgagatc gacaaggcgt cggtaccggc caacccggac ggcgcttcgc
     3841 gcgcccaagc gacacgcaag gcagcactcg ccgaaatcgg gccggcgcta caggcgcagt
     3901 tgcgtgcggt ggagataatc cgcgacgcgc tgcccggctc gatcatcgtc ggcgattcga
     3961 cgcagccgat ctatgccgca aacctctatt acgaccatga ccgccctggc ggctggttca
     4021 atgccgccac cggtttcggc gcgctgggct acggcccgcc ggcggccatc ggcgctgccc
     4081 ttgccgtgcc cggcgctgcc gtcgtgtgcc tgaccggcga tggcggcttt caattcacgc
     4141 tgccggaact gggcgcggca ctggatgcgg atgcgccggt tatcttcgtc gtctggaaca
     4201 atcgcggcta ccgcgagatc gaaacctcga tgcgcgacgt cggtgtcgag ccggtcggcg
     4261 tttcaccggc gccaccggat ttctgcaaac tggccgaagc ctacggcatc gacgccgagc
     4321 ggctttccaa catggacggg ctggctgacg ccttgcgcaa ggcgcgagcg acgaagcgag
     4381 cctatgtaat cgagatttcg gttgagtgac gccacggaag gcgcaacaat tccagccgta
     4441 ttcgcggagc gatcgatcat gacagcatcc ttgcagggca aacacatcgt cattaccggc
     4501 ggcaccggcg cgctcggcgg aaccgtcgtc gccaaactgc tgagcgaggg ggccgtgtgc
     4561 catgtgccga acgcacatgc gacagcgccc gcccatttca gctacgccgg caatgacagg
     4621 gtcaagcttg caaacaatgt cgacctggct gatccggtca aggtcgatgc cttctatgcc
     4681 cgcatcccgg aactatgggc ttcgatccat ctggccggtg gctatgccgg ggcgcccatc
     4741 gaggcgacgg accccgcctc cttcgacgag atgatggaca ccaatgcgcg caccaccttc
     4801 ctgtgctgcc gcgcggccgt gcgttccatg ctggcatcgg gcgtggccgg gcgcatcgtc
     4861 aatgtcagcg cgcgtccggg cctcgacccg aggcgcggcg cgggcatggt cgcctatgcg
     4921 gcaagcaagg cggccgtcgc ggcaatgacg gtggcgctgg ccgaggaggt caagggcaac
     4981 ggcatcctgg tcaatgcaat cgcgccctcg acgctggata cgccggccaa ccgcgccgcc
     5041 atgcagaaag ccgatttcac caggtgggtc gatccggaag ccatcgccga aaccgtggcg
     5101 gcgctggtgt cggcgcgcaa cattctgacc agcggcgcgc tgatcccgct ctatgggcgg
     5161 gcctgagcga cagcgccggt ttaattggat accggagcgc tacagcgtct aagcagggaa
     5221 tcgttcatcc cgacgatctt gccgaccgcg ctgtcgcgtt tgcggcgctc attcgagcgg
     5281 aaatcgggaa ttaccacgcc gaaaccggtg gagaccgggg taaacgtcac cttggcgccg
     5341 gtcggcacgc cgacttctcg cgccagcgcg ttgcgttcat tggtcagccg gccgcatgga
     5401 accttgtcgt acaccagcga cacgaccatg acggaatctt cggcgtcggc cagcgagtgc
     5461 ccggcgcaac cgccaaggcc gagcagcgca accatcaaca tcatgtattt catcaaggac
     5521 cctgaattgt cggaacacat aaaagcatcg cggccggcag gagtcgaatc ctgatggcga
     5581 ccaacgccgg cgcacgaaaa tatgccggcc aatcttgttc atgccttggc gaacggccgt
     5641 cacgctaagc tgtttcctat cgacaagcgc gcgatagaaa gctagggcca aatcaggaag
     5701 ccaagcggaa gcgagaccgg acatgaccgc caagaccctt atcgcgccat cggtgctggc
     5761 atgcgacttt tccaggcttg gcgacgaggt cgaggcggtg acgcaggcgg gcgcggactg
     5821 gatccatctc gatgtgatgg acgggcattt cgtgcccaac atcaccttcg gcccgccggt
     5881 gatcaaggcg atccgcaacc ggaccaaggc gttcttcgac tgccacctga tgatcgagcc
     5941 ggcggacccc tatctggccg ccttcgccga ggccggctgc gacggcatga ccgttcacgc
     6001 ggaggccggc ccgcatctcg atcgttcgct gcaaaccatc cgcaatctgg gcaagaaagc
     6061 gggcgtcgcg ctcaacccgg caacaccgga aagcgccatc gaatatgtgc tcgaccggct
     6121 cgacctcatc ttggtcatga cggtcaatcc gggctttggc gggcaggcct tcatagcggc
     6181 gatggtcgac aaggtccggc gcgtcaaggc gatggtcggc gacaggccga tccacatcca
     6241 gatcgacggc ggcgtgtcgc cgcaaaccgc gccgctggtc acggcggcgg gcgccaacgt
     6301 gctggtggcg ggcgccgccg ttttcaaagg cgacagcgtg gacgcctatc gccggaatat
     6361 ctcggccatc cgcagcgccg ccgacgccgc gatctgacat ttaggccgcg aaaaccggcc
     6421 tgccgtcgat ccacacgccc tgcacgtcga gagcgctcga cagcgcaact atgtttgctg
     6481 cggttccctt cgccagccgg ccgatccggt tcgactggcc gaccgcctgc gccgggtaga
     6541 gcgaagccat gcgcaacgcc tcgccaaggt cgaggccgat agcgcggtgg acgaagcgca
     6601 cggcggaaat catgtcgaga tcggcgcccg ccagcgtgcc gtcggcaagg gtcagtcgcc
     6661 catccttacg gtgaatggtg cggccgttca gtgtgaacga tttcatgtcg gtgccaatcg
     6721 tcgccatcgc gtcggtgacc aggaaaacct tcgccggtcc ccttttggcg cgcagggcaa
     6781 tcgccatcgt cgccggatcg acatggatgc cgtccgcgat cagcccggcg tgaagagagc
     6841 cgccgtcaat cgccgcgccg gccaatcccg gctcccggtt gccgatctgg ctcatggcgt
     6901 tgaacagatg ggtgaccatc gacgcgccgg cttcggcata ggcgttcgcg gccgcatggc
     6961 cgacatcgga atggccaagg ctgacaacaa cgccagcctg cgccagggct gaaactcgct
     7021 ggaggtcgac agattccggc gcgatcgtgg tcaacagcac cggcaggtga gcgcacccag
     7081 cgatcaacgc ggcaaggtca tcgtccgtca tcgggcggat caaggcggga tcgtgcgcgc
     7141 ccttgcgcgc cagcgacaga tgcggccctt ccagatgcag gccaaggaag cccggcacct
     7201 gttgccgccc ggcctcaatg ccggccgcaa tcgctgccgc cgtcacttcc ggcgtatccg
     7261 tgatcagcgt aggcagcagc gccgtggtgc cgaacggtgc atgcgcacgg catattttct
     7321 cgatggaagc cacatccgga tggtcgttca gcatgacgcc gccgccgcca ttgacctgga
     7381 ggtcgacaaa gccgggggcc agaatgccgc cgccggtttc tttcttttcg atctccgccg
     7441 gtagcgaagc ggcctggacg atgccctcga cctcgccgcc attgacgagc agcgcggcgt
     7501 catcgtgcca cgcatcgccg tcgaagatgc gcgctccgat cagggcaaag cgctcgctca
     7561 tcgggtttcc gttaccttac gcaggttggg cggcgtatcg ggatcgaggc cgcgatggcg
     7621 ggcgaaagcc tcgacaaagg catagaatga aacgatcaac gccagcggat cggtcagcgg
     7681 atggccggtg gcggcaaatg gcagatgatg cgccttgccg gcggcggcgc tggtcacgaa
     7741 caccgacgcg cccttgccgg ccaggccttc gcaggcctca atcatggaag gttccgaggc
     7801 atcgcgcgca gccagtgcca gaaccggaaa tcccggaccg acccgcgcca gcggtccatg
     7861 catgacttcg gcggcgctgt aggcttccgc atgcacaccg caggtttcct tgaatttcag
     7921 cgccgcttcg ctggcgatgg cgaaggaagg gccacgcccg agcacgaaca gcgacctgtc
     7981 ttgcg
//
LOCUS       DASYCS010000041         1646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_297834,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000041 DASYCS010000000
VERSION     DASYCS010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297834"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..110)
                     /locus_tag="VGM46_01695"
     CDS             complement(<1..110)
                     /locus_tag="VGM46_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEY4191334.1"
                     /translation="MGSLKIENVKKAFGPVEVLKGIDLEVTDGEFVVFVGP"
     gene            complement(121..1182)
                     /locus_tag="VGM46_01700"
     CDS             complement(121..1182)
                     /locus_tag="VGM46_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY4191335.1"
                     /translation="MSEQAPLSVVVAGLGNMGRSHALAYHGNPGFRIAALVNRSDVAL
                     PAELSGYGIRRSFEEALRDEKPDIACIATYSDSHADYAVRALEAGCHVFVEKPLATTV
                     ADARRVVDAAKANGRKLAIGYILRHHPSWVRLITEARKLGGPYVFRMNLNQQSSGATW
                     QTHKQLMQTTSPIVDCGVHYLDVMLQITDAKPVEVRGMGLRLTDEIAEGMYNYGHLQV
                     LFDDGSVGWYEAGWGPMISETAFFVKDVISPNGCVSIIMKEGAKSDDIDTHTKTSTIR
                     LHSAVTGADGKFTKPDELLSMQGEPGHQELCDLEQAFLLKAIREDIDLTRHMDDAVRS
                     LAVCLAADESVRSGLAVKL"
     gene            complement(1179..>1646)
                     /locus_tag="VGM46_01705"
     CDS             complement(1179..>1646)
                     /locus_tag="VGM46_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015314650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY4191336.1"
                     /translation="ATVAILQLMVASGLVNTLTALVLVYTAQGLPLAVFILSEFMKLV
                     SDDLKNAGRIDGLSEYRIFFRLVLPLIRPSLATVAVFTMIPIWNDLWFPLILAPAEST
                     KTVTLGAQLFLGQFVTNWNAVLAALSLAILPVMILYLIFSRQLIRGITAGAVK"
BASE COUNT          334 a          511 c          496 g          305 t
ORIGIN      
        1 ggaccgacga aaacgacgaa ctcgccatcc gtcacttcca ggtcgatgcc tttcagcacc
       61 tcaaccgggc cgaacgcctt tttcacattc tcgatcttga gagatcccac ggcatgttcc
      121 ctatagtttg acggcaagac cgctgcgcac gctttcatcg gcggcgaggc agacggcaag
      181 cgaacggacc gcatcgtcca tgtggcgcgt caggtcgata tcttcgcgga tcgccttgag
      241 caggaacgcc tgttcgaggt cgcacagttc ctggtggcca ggctcgccct gcatggaaag
      301 cagttcgtcc ggcttggtga atttgccgtc cgcgcccgtc accgcgctat gcaggcgaat
      361 cgtcgatgtc ttcgtatggg tgtcgatgtc gtcggacttc gcgccctctt tcatgatgat
      421 cgacacgcag ccattgggcg agatcacatc cttcacgaag aaggccgtct ccgaaatcat
      481 cggcccccag ccggcttcgt accagccgac cgaaccgtca tcgaacagca cttgcagatg
      541 gccgtaatta tacatgccct cggcaatctc atcggtcagg cgaagcccca tgccgcgcac
      601 ctcgaccggc ttggcgtcgg tgatctgcaa catcacgtct agataatgca cgccgcaatc
      661 gacgatgggc gaggtggtct gcatcaactg cttgtgcgtc tgccacgtcg caccggaaga
      721 ctgctggttg aggttcatgc ggaacacata tggcccgcca agcttgcgcg cttcggtaat
      781 cagccgcacc caggaagggt ggtggcgcag tatgtagccg atcgccagct tcctgccgtt
      841 ggctttcgcc gcatcgacca cgcgccgcgc gtcggcaacg gttgtcgcca gcggcttttc
      901 cacaaacaca tggcagcccg cctccagcgc ccgcacggcg tagtcggcgt ggctgtcgga
      961 ataggtggcg atgcaggcga tatcgggctt ttcgtcgcgc agcgcctctt cgaaggagcg
     1021 gcggatgcca tagccggaaa gctccgccgg caacgccaca tcagaacggt tgaccagtgc
     1081 ggcgatgcga aagcctggat tgccatggta ggcgagcgca tggctgcggc ccatattgcc
     1141 gagaccggcg acgacgacgc tgaggggtgc ctgttcgctc atttgaccgc tccagccgtg
     1201 atgccgcgga tcaactgccg cgagaagatg agatagagga tcatcaccgg caggatggcg
     1261 agtgaaagcg ccgccagcac cgcattccag ttggtgacga actggccgag gaaaagctgc
     1321 gcgccaagcg tcaccgtctt ggtgctttcg gccggcgcaa ggatcagcgg gaaccacaga
     1381 tcgttccata tcgggatcat ggtgaagacg gcgacggtgg ccagcgatgg ccggatcagc
     1441 ggcaaaacca gccggaagaa gatgcggtat tccgacagcc cgtctatgcg cccggcattc
     1501 ttcaaatcgt cggacaccag cttcatgaac tcggaaagaa taaacaccgc cagcggcaac
     1561 ccttgcgccg tatagaccag caccagcgcg gtgagcgtgt tgaccagccc gcttgccacc
     1621 atcagttgca ggatggcgac ggtggc
//
LOCUS       DASYCS010000042         6879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3487193,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000042 DASYCS010000000
VERSION     DASYCS010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 6879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6879
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3487193"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="VGM46_01710"
     CDS             <1..597
                     /locus_tag="VGM46_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006206263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HEY4191337.1"
                     /translation="DEVVHGKGTLLGKMAGDDWQKFATLRAFYAFMWGYPGKKLLFMG
                     QEFAQRREWSEARSLDWNLLDFEPHRGMRDTVRDLNRLYRSRPALHARDCEPEGFSWL
                     IVDDSANSVFAWLRSAPGENPVAVISNFTPVARENYRVPLPRAGLWREIINTDASEYG
                     GSGQGNNGAVEAGGEGGNVSATMLLPPLATIMLEFVPR"
     gene            617..1882
                     /gene="glgC"
                     /locus_tag="VGM46_01715"
     CDS             617..1882
                     /gene="glgC"
                     /locus_tag="VGM46_01715"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEY4191338.1"
                     /translation="MVETKRTQPLARDAMAYVLAGGRGSRLKELTDRRAKPAVYFGGK
                     TRIIDFALSNALNSGIRRLGVATQYKAHSLIRHLQRGWNFLRPERNESFDILPASQRV
                     SETQWYEGTADAVYQNIDIIESYGPEFMVILAGDHIYKMDYELMLRQHVDAGADVTVG
                     CLEVPRLEASGFGVMHVDQKDNIISFIEKPADPPAMPDKPDSALASMGIYVFRTKFLM
                     EMLRKDAADPNSSRDFGKDIIPEIVKGGKAIAHRFTKSCVRSNSEAQAYWRDVGTVDA
                     YWEANIDLTDITPELDLYDREWPIWTYAELKPPAKFVHDEDGRRGSAVSSLVSGDCIV
                     SGASLRRSLIFTGARINSFSTLDEVVMLPDCTIGRNVHLKRVVIDHGVKIPEGLVVGQ
                     DPILDAKRFRVSEKGICLVTQAMVDRLEL"
     gene            1886..3331
                     /gene="glgA"
                     /locus_tag="VGM46_01720"
     CDS             1886..3331
                     /gene="glgA"
                     /locus_tag="VGM46_01720"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006206261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEY4191339.1"
                     /translation="MQVLSVTPEIYPLIKTGGLADVTGALPIAVAGKGVAMRTLVPGF
                     PAIMAAFKKKKAVLAYPSLQGGKAAVHAVQLAGLDLFVLDAPHLFDRPGGPYGDTTGA
                     DWPDNWRRFAVFGQVGADIAGGAISGYMPDIVHAHDWQSAMTLAYMRYGKAVGTPSLI
                     TVHNLAFQGQFGAGIFAELGLPAAAMALDGVEYYGGVGFLKAGLQAAWAITTVSPTYA
                     QEIRSAEFGMGLDGLITLRANDLHGIVNGIDTDVWNPQTDAHLASTYSVKTLKARNAN
                     KTAVEERFSLDRDASPIVCVVSRLTWQKGIDTLVGTIDELVAAGVRLAVLGSGDAGLE
                     GALLAAAARHRGRIGVVIGYDEGLSHLMQGGCDAIVIPSRFEPCGLTQLYGLRYGCVP
                     VVARTGGLADTVIDANEAAIWAGVATGFMFAPNDAGSLLHAIQRVAAIHQNPVMWASL
                     QRQGMKADVSWDRSAQKYVELYSQLLSKKAR"
     gene            3335..4963
                     /locus_tag="VGM46_01725"
     CDS             3335..4963
                     /locus_tag="VGM46_01725"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020051644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HEY4191340.1"
                     /translation="MIRTVPTKPYNDQKPGTSGLRKKVPEFQQEHYAENFIQSIFDAL
                     DGFKGKTLVIGGDGRYYNREVIQKALAIAAANGFGKVMVGQGGILSTPAASHVIRKYK
                     AFGGIILSASHNPGGPHGDFGIKYNAENGGPAPEKITDAIFAKSKTISHFKTADIGAI
                     DIDTVGIVVAEGMTIEIIDPVKDYAEMMESLFDFKALRKLFESGFRMRFDAMHAVTGP
                     YAKEILEHRLGAADGTCRNFKPLPDFGGHHPDPNLVHAKHLYDEMMGDNAPDFGAASD
                     GDGDRNLIIGKGIFITPSDSLAMLAANATVAPGYKDGLAGIARSMPTSGAADRVAEKL
                     GVGIYETPTGWKFFGNLLDAGMATICGEESAGTGSNHVREKDGLWAVLLWLSILAARG
                     ESAREIASKHWATYGRNYYSRHDYEEVESDKANALVDELRAKLATLPGTKVRGLTVEK
                     ADDFAYHDPVDHSVSEHQGIRVLFKGGSRVVFRLSGTGTTGATLRVYIERYEPDPAKH
                     DIDTQEALADLIAAADDIAGIHSHTGRPKPSVIT"
     gene            4984..>6879
                     /gene="glgX"
                     /locus_tag="VGM46_01730"
     CDS             4984..>6879
                     /gene="glgX"
                     /locus_tag="VGM46_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018426923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEY4191341.1"
                     /translation="MWTQPRFGSLVHADGIRFAAWSQAAQRIWVSVFDGEGSREMDRL
                     ELTPEGGGVHGLFAPGLGGGTRYGLRADGEYAPERGLWFDPQKLLVDPYAIEIDRPYA
                     YDPRLATRRGEGADTSPLMPKTIALDLATAAASAPPLFQPGGLIYEVAVRAFSMRHPG
                     VPAQDRGTIRALASPAVIEYLQRLGVSAVELMPVTAKIDERHLPPLGLTNAWGYNPVT
                     FMALDPRLAPGGLADLRETTAALRQAGIGVILDLVFNHTGESDRLGPTLSLRGLDAQA
                     CYRHGADGALVNDTGTGNTVACDHPFMRELILDSLRHFVRNAGVDGFRFDLAPVLGRV
                     DGVFEPHAPLLEAMRNDPLLADRVLIAEPWDIGPGGYQLGNFTPPFLEWNDKYRDDVR
                     RFWRGDSGMVGALATRLAGSSDVFSSNGQASTRTVNFIAAHDGMALADIAAYVGKHNE
                     ANGEQGRDGHDENLSWNNGVEGGSIDPTIARQRRNDVRAMLATLFASRGSIMLTAGDE
                     FGRSQQGNNNAYAQDNEITWLDWSGRDRKLEDHAFALAALRRAMPVLRDTRFLTGEPQ
                     ALGDNPDVEWLAETGRLLEQADWNDPGRRRLVMLLDGGEGQRLAVLVNGDRRACQFTL
                     PKRAGHAW"
BASE COUNT         1253 a         2208 c         2187 g         1231 t
ORIGIN      
        1 gacgaggtcg tgcacggcaa gggcacgctg ctcggcaaga tggccggcga cgactggcag
       61 aaattcgcca cgctgcgcgc cttctacgcc ttcatgtggg gctatccggg caagaagctg
      121 ctgtttatgg gccaggaatt cgcccaacgg cgcgaatggt cggaggcgcg ctcgcttgac
      181 tggaatctcc tggatttcga gccgcatcgc ggcatgcgcg acaccgtgcg cgacctcaat
      241 cggctctacc gctcacgccc ggcgctgcat gcgcgcgatt gcgagccgga gggcttttcc
      301 tggctgatcg tcgatgacag cgccaactcc gtctttgcct ggctgcgcag cgcgccgggt
      361 gaaaacccgg ttgcagtcat cagcaacttc acgccggtgg cgcgtgaaaa ctatcgcgtg
      421 ccgctgccaa gggcgggttt gtggcgcgag atcatcaaca ccgatgcaag tgagtatggc
      481 ggttccggcc aaggcaataa tggcgctgtc gaagcgggcg gcgaaggcgg caacgtgtcg
      541 gccacgatgt tgttgccgcc gctggccacg atcatgctcg aatttgttcc gcgttagttt
      601 caaggggagg ctgtaaatgg tggagacgaa acggacccag ccgctggcgc gcgacgccat
      661 ggcctatgtg ctggccggcg ggcgcggcag ccggctgaag gaactgacgg accggcgcgc
      721 caagcccgcc gtctatttcg gcggcaagac gcgcatcatc gactttgcgc tgtccaatgc
      781 gcttaattca ggcattcgcc ggctcggcgt cgccacgcaa tacaaggcgc actcgctgat
      841 ccgccacctg caacgcggct ggaacttcct gcggccggaa cgaaacgaaa gcttcgacat
      901 cttgccggcc agccagcgcg tctcggaaac gcagtggtat gagggcaccg ccgacgccgt
      961 ctaccagaac atcgatatca tcgagagcta cggcccggaa tttatggtca tcctggcggg
     1021 cgaccacatc tacaagatgg attacgagct gatgcttcgc cagcacgtcg atgccggcgc
     1081 ggacgtgacg gtcggctgcc tggaagtgcc gcgcctggaa gccagcggct ttggcgtgat
     1141 gcatgtcgac cagaaggaca acatcatctc cttcatcgag aaaccggccg atccgccggc
     1201 catgccggac aagccggact ccgccctggc ttcgatgggc atctatgtgt tccgcaccaa
     1261 attcctgatg gaaatgctgc gcaaggatgc cgccgatccg aattcgagcc gcgatttcgg
     1321 caaggatatc attccggaaa tcgtcaaggg cggcaaggcg atcgcgcacc gctttaccaa
     1381 gtcgtgcgtg cgctccaatt ccgaggcgca agcctattgg cgcgacgtcg gcacggtgga
     1441 tgcctactgg gaagccaata tcgacctcac cgatatcacc ccggaactcg acctctacga
     1501 cagggaatgg ccgatctgga cctatgccga attgaagccg ccggcaaagt tcgtgcatga
     1561 cgaggatggc cggcgcggct ccgccgtttc gtcgctggtg tcgggcgact gcatcgtttc
     1621 gggcgcttcg ctgcggcgaa gcctgatctt caccggcgcg cgcatcaatt ccttctcgac
     1681 gctggatgag gtggtgatgc ttcccgactg caccatcggc cgcaatgtgc atctgaagcg
     1741 cgtcgtcatc gaccatggcg tcaagatacc ggagggcctg gtggtcggcc aggacccgat
     1801 acttgacgcc aagcgcttcc gggtctcgga aaaaggcatc tgcctggtta cgcaagccat
     1861 ggtcgacagg ctggaactgt aaaaaatgca ggtgctttcg gtcacgccgg aaatctatcc
     1921 gctcataaag accggcggcc tcgccgacgt gaccggcgcg ctgcccatcg ccgtcgccgg
     1981 caagggcgtg gcgatgcgca cgcttgttcc gggttttccc gcgatcatgg cggcgttcaa
     2041 gaaaaagaag gcggtgcttg cctatccctc gcttcaaggc ggcaaggccg ccgtgcatgc
     2101 cgtgcaactg gccgggcttg acctgttcgt gctcgacgcg ccgcatcttt tcgaccgtcc
     2161 gggcggcccc tacggcgata cgacgggcgc ggactggccg gataattggc gccgcttcgc
     2221 cgtattcggc caggtcggcg ccgatatcgc cggcggcgcc atctccggct acatgcccga
     2281 catcgtccat gcgcatgatt ggcagtcggc gatgacgctg gcctatatgc gctacggcaa
     2341 ggcggtcggc acgccctcgc tcatcaccgt gcataatctg gctttccagg gccagttcgg
     2401 ggcgggcatc ttcgccgagc ttggcctgcc ggcggcggcg atggcgctgg acggggtgga
     2461 atattacggc ggcgtcggct tcctcaaggc agggttgcag gcggcctggg ccatcaccac
     2521 cgtatcgccc acctatgcgc aggaaatccg ctcggccgaa ttcggcatgg ggctggacgg
     2581 cctgatcacc ctgcgcgcca acgatctgca cggcatcgtc aatggcatcg acaccgatgt
     2641 gtggaacccg cagacggatg cgcatctggc ttcgacctat tcggtgaaaa ccttgaaggc
     2701 gcgcaacgcc aacaagacgg cggtggagga gcgcttcagc ctcgaccgcg acgccagccc
     2761 catcgtctgc gtggtcagcc ggctgacctg gcaaaaaggc atcgatacgc ttgtcggcac
     2821 catcgacgaa ctggtcgccg ccggtgtgcg tcttgccgtg ctaggctccg gcgatgccgg
     2881 ccttgaaggc gcgctgctgg ccgccgccgc ccgccatcgc ggccgtatcg gcgtcgtcat
     2941 cggctatgat gaaggcttgt cgcatctcat gcagggcggc tgcgatgcca tcgtcatccc
     3001 gtcgcgtttc gagccgtgcg gtctgaccca gctttacggc ctgcgctatg gctgcgtgcc
     3061 ggtggtggcg cgcaccggcg gcctggccga tacggtcatc gacgccaacg aggcggcgat
     3121 ttgggcgggc gttgcaaccg gcttcatgtt cgcgcccaat gacgccggct cgctactcca
     3181 tgccatccag cgggtggcgg ccatccatca aaacccggtg atgtgggcat cgctccagcg
     3241 tcaaggcatg aaagccgatg tatcgtggga tcgaagcgcg caaaaatacg ttgaactcta
     3301 ttcgcagctt ctatcgaaaa aggctcgatg acgcatgatc cgcacagtcc cgacaaagcc
     3361 ctataacgac cagaagcccg gcacgtcggg cctgcgcaag aaggtgccgg agttccagca
     3421 ggaacattat gccgagaact tcatccagtc gattttcgat gcgctggacg gcttcaaggg
     3481 caagacgctg gtgattggcg gcgacggccg ctactataat cgcgaagtca tccagaaggc
     3541 gctggccatc gccgccgcca acggctttgg caaggtcatg gtcgggcagg gcggcatcct
     3601 gtcgacgccg gcggcgtcgc atgtcatccg caaatacaag gccttcggcg gcatcatcct
     3661 gtcggccagc cataatcccg gcggcccgca tggcgatttc ggcatcaagt acaatgccga
     3721 aaacggcggc ccggcgcccg agaaaatcac cgacgccatc ttcgccaagt cgaagaccat
     3781 ctcgcatttc aagaccgccg acattggcgc catcgacatc gacacggtcg gcatcgtcgt
     3841 tgcagaaggc atgactatcg agatcatcga tccggtcaaa gactatgccg agatgatgga
     3901 aagcctgttc gatttcaagg cgttgcggaa gctttttgaa tcaggtttcc gcatgcgctt
     3961 cgacgccatg cacgcggtga ccggccccta tgccaaggaa atcctcgaac acaggttggg
     4021 cgcggcggac ggcacctgcc gcaatttcaa gccgctgccg gatttcggcg gccaccaccc
     4081 cgatcccaat ctcgtccacg ccaagcatct ctatgacgag atgatgggcg acaatgcgcc
     4141 ggacttcggt gcggcctccg atggtgacgg cgaccgcaac ctgatcatcg gcaaaggcat
     4201 cttcatcacg ccctcggatt cgctggcgat gcttgccgcc aacgccactg tggctccggg
     4261 ctacaaggat ggcttggcgg gcatcgcccg ctccatgccg accagcggcg ctgccgaccg
     4321 cgttgccgaa aagcttggcg tcggcattta tgaaacgccg accggctgga aattcttcgg
     4381 caatctgctc gatgccggca tggcgaccat ctgcggcgaa gaaagtgcgg gcaccggttc
     4441 caaccatgtc cgcgagaagg acgggctgtg ggccgtgctg ttgtggctct ccattctggc
     4501 cgcgcgcggc gaaagcgcca gggagatcgc cagcaagcac tgggcgacct atggccgcaa
     4561 ttactattcc cgccatgact atgaggaggt cgagagcgac aaggccaatg cgctggtcga
     4621 cgagttgcgc gccaagctcg ccacgctgcc cggcacgaag gtgcgcgggc tgactgtcga
     4681 gaaggccgac gacttcgcct atcacgatcc ggtcgaccac tccgtcagcg agcatcaggg
     4741 tatccgcgtg ctgttcaagg gcggctcccg cgtcgtgttc cgcctgtccg gcaccggcac
     4801 caccggcgct acgctgcgcg tctatatcga aagatatgaa ccggacccgg cgaagcacga
     4861 catcgacacg caggaggcgc tggccgatct gatcgccgcc gccgacgaca ttgccggcat
     4921 ccacagccac acgggccggc ccaagccgag cgtcatcacc tgaaccccag gcagcagctt
     4981 tccgtgtgga cccaaccaag gtttggaagt ctcgttcatg ctgacggcat tcgttttgcc
     5041 gcctggtcgc aagcggccca gcgcatctgg gtgtcggtct tcgatggcga gggaagccgg
     5101 gagatggacc ggcttgagct gacgcctgaa ggcggcggcg tgcatggcct gttcgcgccc
     5161 ggtcttggcg gcggcacgcg ctacggcctg cgcgccgatg gcgaatatgc gccggagcgc
     5221 ggcctgtggt tcgatccgca aaagctgctg gtcgatccct atgccatcga gatcgaccgg
     5281 ccttacgcct acgatccgcg ccttgccacc cggcgcggcg agggggccga cacctcgccg
     5341 ctgatgccca agacgatcgc gctggatttg gccacggcgg ccgcttcggc cccgccgctc
     5401 ttccagccgg gcgggctgat ctatgaggtg gcggtgcgcg ccttttccat gcgccatccc
     5461 ggcgtgccgg cacaggatcg cggcacgatc cgcgcgctcg cttcgccggc ggtcatcgag
     5521 tatttgcaaa ggcttggtgt gtccgccgtc gaactgatgc cggtgaccgc caagatcgac
     5581 gaacgccatt tgccgccgct cggcctgacc aatgcctggg gctacaaccc ggtgaccttc
     5641 atggcgctgg acccccgcct cgcgccggga ggcctggccg atttgcgcga aacaactgcc
     5701 gcgttgcgcc aggccggcat cggcgtcatc ctcgacctcg tcttcaacca taccggtgaa
     5761 agcgacaggc ttgggccgac tttatcgctg cgcgggctgg acgcgcaggc ctgttaccgg
     5821 cacggcgccg atggcgctct ggtcaacgac accggcaccg gcaatacggt cgcctgcgac
     5881 catcctttta tgcgcgagct tattctcgac agcctgcgcc atttcgtgcg caatgccggc
     5941 gtcgatggct tccgcttcga tctcgcgccg gttctcgggc gggtcgatgg tgttttcgag
     6001 ccgcacgcgc cgcttctgga ggccatgcga aacgaccctc tgcttgccga ccgggtgctg
     6061 atcgccgagc catgggacat cgggccgggc ggctatcagc tgggcaattt cacgccgcct
     6121 ttcctcgaat ggaacgacaa ataccgcgat gacgttcgcc gtttctggcg cggcgacagc
     6181 ggcatggtcg gcgcgctggc aacgcggctt gcgggatctt cggacgtgtt ttccagcaac
     6241 ggccaggcaa gcacccgcac cgtcaatttc atcgccgccc atgacggcat ggcgctggcc
     6301 gatatcgccg cctatgtcgg caagcataat gaggccaatg gcgaacaggg ccgtgacggt
     6361 cacgacgaga acctttcctg gaacaatggc gtcgagggcg ggagcatcga cccgacgata
     6421 gcgcgccagc gccgcaacga tgtgcgcgcc atgctggcca cgctgtttgc ctcgcgcggt
     6481 tcgatcatgc tgaccgccgg cgacgagttt ggccgaagcc agcaaggcaa caacaacgct
     6541 tatgcgcagg acaatgaaat tacctggctg gactggagcg gacgcgaccg caagctggaa
     6601 gaccacgcct ttgcgctagc ggcgctgcgc cgcgccatgc cggtgctgcg cgacacgcga
     6661 ttcctgactg gcgaaccgca agcactcggc gacaatccag acgtggaatg gctcgccgag
     6721 acaggccggc tgctcgaaca ggccgattgg aacgatccgg ggcgacgtcg cctggtcatg
     6781 ctgcttgacg ggggcgaggg gcagcggctt gccgttcttg tcaacggcga ccgccgcgcc
     6841 tgccagttca cgctgccgaa gcgcgcgggc catgcatgg
//
LOCUS       DASYCS010000043         4047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1457619,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000043 DASYCS010000000
VERSION     DASYCS010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1457619"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="VGM46_01735"
     CDS             complement(<1..783)
                     /locus_tag="VGM46_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006201912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEY4191342.1"
                     /translation="MITSRRLFLSGVSALAATVVAGGARAQDVVDDILKSSAQGNWDD
                     TFDARVSSGGKVASTLPIFSPQTISFTEQAIAQYQGIVSQGGWEQVPATKKLQLGVID
                     ADVVPLRKRLMVSGDLPRSAGLSQSFDSYVDAAVKRFQARHGLPSDGVLGKYSYAAMN
                     VAAQTRLGQLNTNLGRLREKVGTLGERYVLVNIPAAQVEAVENGRVVLRHTAIVGKID
                     RQTPIVNSKINEIIVNPYWNAPVSIVRKDIIPLMRKDPSYLKD"
     gene            complement(1215..1730)
                     /locus_tag="VGM46_01740"
     CDS             complement(1215..1730)
                     /locus_tag="VGM46_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HEY4191343.1"
                     /translation="MINSRQVEKTAIVSDDRREAIRSLYMESLQLVERLHRRLLDVIK
                     DEFDRNGRSDINAIQALLLFNIGNSELTAGELRSRGYYLGSNVSYNLKKLVDLGFINH
                     QRSRIDRRSVRVSLTDKGNEVAEVVAGLYERHVGSIEQVGGINTDEFKQMNRALQRLD
                     RFWNDTIAYRM"
     gene            complement(1934..2356)
                     /locus_tag="VGM46_01745"
     CDS             complement(1934..2356)
                     /locus_tag="VGM46_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008875358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6163 family protein"
                     /protein_id="HEY4191344.1"
                     /translation="MSDDLSRQVVLSPSLAEVLFAWFQRIIAAYCLLFGILYWIKLIG
                     FYHAPLWRFDLMPVHWQVAATVLAVFFPFAAAGLWMMASWGPVIWVICAVTETIMYAG
                     LPALFGRNWGIVSSHIAVMALYVAFRFILHAQKRRAGQ"
     gene            complement(2353..3396)
                     /locus_tag="VGM46_01750"
     CDS             complement(2353..3396)
                     /locus_tag="VGM46_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006332588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY4191345.1"
                     /translation="MDFGGGDEIRFECQGSVGIVTLTRPQALNAITHKMVKALSKALH
                     AWERDKAISLVLVKAEGRAFSAGGDIVQAYKAGRAGSPPVEFFADEYRLNAQIDRFKK
                     PYVALIDGIVMGGGAGISVHGSHRVMTENAVFAMPEVGIGFFPDVGASHVLPDLGGSF
                     GFYLGLTGNRIKYGDALWSGLATHTIKAADQAGFIERLVEVGEAESVLRNFFFPARRE
                     TDRPTLESIARHFSQPSLADIVSSLEHAAQGDEFAAATLATIRTRSPTSLSVAWREIN
                     SGQTLSMDECMRMEFRILNRVLQGHDFYEGVRAALIEKDGKPKWHPASLDAVQSADVD
                     AYFAPLGERELTP"
     gene            3592..>4047
                     /locus_tag="VGM46_01755"
     CDS             3592..>4047
                     /locus_tag="VGM46_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006201919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEY4191346.1"
                     /translation="MNKFTQTRPAGARNVDEITGSRRLRRMRRAPWSRRLVQENHLTV
                     DDLIWPIFLIEGTNIREPVEAMPGVFRLSVDLAAKEAERAAKLGIPAIATFPNVDMKL
                     RDQTGSHILDPDNLINRATRAMKHAAPEVGIITDVALDPFTSHGHDGILR"
BASE COUNT          794 a         1245 c         1250 g          758 t
ORIGIN      
        1 gtccttgagg tagctagggt ccttgcgcat caacgggatg atgtccttgc gcacgattga
       61 aaccggcgca ttccaatatg gattgacgat gatctcgttg atcttggaat tgacgatcgg
      121 ggtctggcga tcgatcttgc caacgattgc ggtatggcgc agcacgacgc ggccgttttc
      181 aaccgcctcg acctgggccg cgggaatgtt gaccagcacg tagcgctcgc cgagcgtgcc
      241 gaccttttcg cgcagcctgc ccagattggt gttgagctgg ccgaggcgcg tctgcgcggc
      301 gacgttcatc gccgcgtagg agtattttcc aagaacgcca tccgaaggca ggccgtggcg
      361 cgcctggaag cgcttgacgg cggcgtcgac gtacgaatcg aaggattgcg aaagaccggc
      421 gctgcgcggc aggtcgcccg aaaccatcag gcgcttgcgc agcggcacca cgtcggcatc
      481 gattacgcca agctgcaatt tcttggtcgc cggcacctgt tcccagccgc cctgcgagac
      541 gatgccctga tattgcgcaa tagcctgctc ggtaaaggaa atcgtctgcg ggctgaagat
      601 cggcagcgtc gaggcgacct tgccgcccga actgacacgc gcatcgaacg tgtcgtccca
      661 attgccctgc gccgaagact tcaggatgtc atcgacgacg tcctgcgcgc gggcgccgcc
      721 ggcaacgacc gtggcggcaa gagcggagac tccggaaaga aaaaggcggc ggctggtgat
      781 catgaacgtt ccagacaata tctcgtgacg gttcgatact tcagtattcc gcggccggat
      841 ttcgcggcca ttcaacgacg ggcaactggc aacgacaggc aactggcttc tatttgctgc
      901 ggacaacctt aacaatctgc caatcttggc aggcatccat ccggctcggg caattgccga
      961 cgccattgct tgcgacaatg acgggcttca gcccaaggct ttatcacata ctcgctttca
     1021 tttgaatatg gcggcaacgt ggcgttgccg cccttgccgg gcgcgtggcc atgcaagaaa
     1081 aacgggccag tgctttttat gcaccggccc gcgactggag tttctgcctc gaacccgcgc
     1141 cgcgcgaagc cgtgacgggt ccggtccgcc gcgcatcctc gtgcgccggc agaggaaatt
     1201 cgatggccgc cggattacat ccggtaggcg atggtgtcgt tccagaagcg atccaggcgt
     1261 tgcagcgcgc ggttcatctg cttgaactcg tcggtgttga tgccgccgac ctgttcgatg
     1321 gagccgacat ggcgctcata gagaccagcg acgacttccg ccacctcatt gcccttgtcg
     1381 gtcaggctga cgcggaccga acggcggtcg atgcgcgagc gctggtggtt gatgaagccc
     1441 agatcgacca gcttcttcag gttgtaggag acgttggagc cgagatagta gccacgcgag
     1501 cgcagttcgc cggcggtcag ctcagaattg ccgatgttga acagcagcag cgcctggatg
     1561 gcgttgatgt cggaacggcc gttgcggtcg aactcgtcct tgatgacatc cagcaggcgg
     1621 cggtgcagcc gctccaccaa ttgcagggat tccatataca gcgaacggat tgcctcacgg
     1681 cggtcgtcgg atacgatggc ggtcttttcc acctgacgcg aattgatcat tgtctttgcc
     1741 tctcgtttgt cgccctggtg attttttgtt cttcaccttg atcacgacac tatcgaatac
     1801 tcataaaatt cgacttaaac gccaaggcta acaagagctt accagcaacg gctttagtaa
     1861 gaggcttaat gaacggtcac ccgagacggg actattaacc taaatattta gcgaccggaa
     1921 aacgggcttc gcgctactgc ccggcgcgcc gcttctgggc gtggaggatg aaccggaagg
     1981 cgacgtaaag cgccatgacg gcaatgtgcg aggaaacgat cccccagttc ctgccgaaca
     2041 gtgccggcag gccggcatac atgatggttt cggtgacggc gcagatgacc cagatgaccg
     2101 gcccccacga ggccatcatc cacagaccgg cggccgcgaa cggaaagaac acggcgagca
     2161 ccgtcgccgc cacctgccaa tgcaccggca tcaggtcgaa gcgccacagc ggcgcgtggt
     2221 agaagccgat cagctttatc caataaagga tgccgaacag caggcagtag gcggcaatga
     2281 tgcgctggaa ccaggcgaac aggacttcgg ccagcgatgg gctgagtacg acctgcctgg
     2341 ataaatcatc gctcacggcg tcagttcccg ttcgcccaac ggcgcgaaat aggcgtccac
     2401 atccgcgctt tggacggcgt cgaggctggc gggatgccat ttcggcttgc cgtccttttc
     2461 gatcagggcg gcgcggacgc cttcgtagaa gtcgtggcct tgcagcacgc ggttgaggat
     2521 gcggaactcc atgcgcatgc attcatccat cgacagggtc tggccggaat tgatctcgcg
     2581 ccaggcgacg ctgaggctgg tcggcgagcg cgtcctgatg gtggcgagcg tggcggcggc
     2641 gaattcatcg ccttgcgcgg catgttccag gctcgaaact atatcggcaa gcgagggctg
     2701 cgagaaatgg cgcgcgatcg attccagcgt cgggcggtcc gtttcgcgcc ttgccgggaa
     2761 gaaaaagttg cgcaggacgg attcggcctc gccgacctcg accagccttt cgatgaaccc
     2821 ggcctggtcg gcggccttga tcgtgtgggt tgcaagcccc gaccacagcg catcgccata
     2881 tttgatgcgg ttgccggtaa gccccaggta gaagccgaag ctgccgccga ggtcgggcag
     2941 gacatggctg gcgccgacat ccgggaaaaa gccgatgccg acttccggca tggcgaatac
     3001 ggcgttttcc gtcatcaccc ggtgtgagcc atggacggaa atgccggcgc cgccccccat
     3061 gacgataccg tcgatcagcg ccacataggg cttcttgaaa cgatcgatct gggcattcag
     3121 gcggtattcg tcggcaaaga attccaccgg cgggcttccg gcgcgcccgg ctttatacgc
     3181 ctggacgata tcgccgccgg ccgagaacgc ccggccctcg gccttgacca ggacgaggct
     3241 gatggccttg tcgcgctccc aagcgtgcag cgccttggac agtgccttga ccatcttgtg
     3301 ggtaatggcg ttcagcgcct gcggccgcgt cagcgtgacg atgccgaccg agccttggca
     3361 ctcaaagcgg atttcgtcgc cgccgccaaa atccatgcgc agaatccttt ccttgagccg
     3421 gagccaaact gctaaagggt cgcgtggggc cgcgtcaatg ccgccgggcc gatccgcaca
     3481 gacgctgccc gaacccgcca acgtgccggc ctcattgcgc tggcacaatc gtgtcgcatt
     3541 ggtcttggcc ggaatctcgt ggtaaaagcc gcgccgtccg gagatttcgc catgaacaaa
     3601 ttcacccaga ccaggcctgc gggcgcgcgc aacgtcgatg agatcaccgg ctcgcgccgc
     3661 ctgcgccgga tgcgccgggc gccatggtcg cgccggctcg tgcaggaaaa ccacctgacg
     3721 gtcgatgacc tgatctggcc gatcttcctc atcgaaggca ccaatattcg cgagccggtc
     3781 gaggcgatgc cgggcgtgtt ccgcctctcg gtcgatctgg cggccaagga agccgagcgc
     3841 gccgcgaagc ttggcattcc ggccatcgcc acctttccca atgtcgacat gaagttgcgc
     3901 gaccaaaccg gctcgcatat cctcgacccc gacaatctca tcaaccgggc gacgcgcgcc
     3961 atgaaacatg cggcgcctga agtcggcatc atcaccgatg tggcgctcga cccgttcacc
     4021 agccatggcc atgacggcat cctgcgc
//
LOCUS       DASYCS010000044         8479 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3342552,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000044 DASYCS010000000
VERSION     DASYCS010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 8479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8479
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3342552"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            313..389
                     /locus_tag="VGM46_01760"
     tRNA            313..389
                     /locus_tag="VGM46_01760"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:347..349,aa:Pro,seq:ggg)
     gene            complement(744..1595)
                     /gene="purU"
                     /locus_tag="VGM46_01765"
     CDS             complement(744..1595)
                     /gene="purU"
                     /locus_tag="VGM46_01765"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018429229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HEY4191347.1"
                     /translation="MNDRSFILTLNCVDQSGIVAAVTTELASIGANIVESNQYWDHQT
                     GQFFMRIAFTADSQKTSDDITRSIKPAVDRFGIKTTLTDRTRKPRIIIMVSKFDHALL
                     HLLYQVKVGWLNAEVVAIVSNHEDSRNTAELEGVPYHFWKATKDNKPELDENLLDLVR
                     KTDADLVVLARYMQVLSDNVSKRLYGKIINIHHSFLPSFKGAKPYHQAHERGVKLIGA
                     TAHYVTADLDEGPIVEQETERVSHAMTAEDFVAAGRDVESRVLARAVKKHVEGRVMLN
                     GHKTVVF"
     gene            complement(1880..2182)
                     /locus_tag="VGM46_01770"
     CDS             complement(1880..2182)
                     /locus_tag="VGM46_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191348.1"
                     /translation="MPSFSDMTDIDLEKQIASLSKELAALKKTASKRGKAYYDEGRDA
                     AWDSYSDLAERISDALPGLRKRAKVIEGTARDHPATAAAVGLVVVGLVAAMLFNRR"
     gene            complement(2249..2791)
                     /locus_tag="VGM46_01775"
     CDS             complement(2249..2791)
                     /locus_tag="VGM46_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008875383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY4191349.1"
                     /translation="MDKSPDAFRTISEVAEDLDLPQHVLRFWETRFTQIKPMKRGGGR
                     RYYRPQDVELIKGIRAMLYDQGYTIKGVQKLLRENGNQFLVAIGSGDIAAVEAIAQRK
                     QADAVPLTPAAQPRGPDDEMMVGQPRVKPSRRFFGLGKGDDEGPVPSDASKLSRDNRA
                     LLQEALFDLLECKRLLDQVR"
     gene            complement(2935..3258)
                     /locus_tag="VGM46_01780"
     CDS             complement(2935..3258)
                     /locus_tag="VGM46_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010915609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEY4191350.1"
                     /translation="MGGKTLTRADLAEAVYRKVGLSRTESAELVESVLDEICEAIVRG
                     ETVKLSSFATFHVRSKNERIGRNPKTGEEVPILPRRVMTFKASNVLKSRILRSHQSSK
                     AKAAK"
     gene            complement(3362..4333)
                     /locus_tag="VGM46_01785"
     CDS             complement(3362..4333)
                     /locus_tag="VGM46_01785"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010915608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEY4191351.1"
                     /translation="MIRSLVRGIGASLPRRIMKNADFEGMVATSDEWIAQRTGIRQRH
                     IAADDETTASLGEAAARAALDAAGLVASDIDLIVLATSTPNNTFPATAVEIQNRLGMH
                     NGFAFDMQAVCSGFVYAITTADLYIRGGLAKRALVIGSETFSRILDWSDRSTCVLFGD
                     GAGAVVLEAGEGAGDISDRGVLAASLRSDGVHKEKLYVDGGPSTTGTVGQLRMEGREV
                     FKHAVGMITDVIEATFTEAGITAEDLDWFVPHQANKRIIDASAKKLGIADEKVVVTVD
                     LHGNTSAASVPLALSVAVADGRIKKGDLVLLEAMGGGFTWGAVLVRW"
     gene            complement(4343..5410)
                     /gene="plsX"
                     /locus_tag="VGM46_01790"
     CDS             complement(4343..5410)
                     /gene="plsX"
                     /locus_tag="VGM46_01790"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018427372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEY4191352.1"
                     /translation="MIRISIDAMGGDHGPDVVIPALMTVATRRTDIRFVIYGREEIVR
                     PILARYPKLVEVSELIHCEVAVRMDDKPSAALRNGRWKSSMWKAIEAVKNGTADACIS
                     AGNTGALMAMSKFCLRTMATIDRPAIAAIWPTLRGESVVLDVGATIGADAHQLIDFAI
                     LGTGMARSVFGIDRPTVGMLNVGVEEIKGQEEVKEAGRMLRDANMASLNYVGFVEGND
                     IGKGAVDVVVTEGFSGNIALKTAEGTARQFAQYLREAMSRTLMARIGYIFARGAFNRL
                     REKLDVGRSNGGVFLGLNGIVVKSHGGADSNGFAAAVELGYDMVRNRLLDRIEADLDL
                     FHARNPHAASLRSSDDTEEQE"
     gene            complement(5512..6069)
                     /locus_tag="VGM46_01795"
     CDS             complement(5512..6069)
                     /locus_tag="VGM46_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010915606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEY4191353.1"
                     /translation="MSETSPKSPVSFSVHVARLPQKGLQVTVEADSAQKAALAEEHGL
                     LSVENWRAELDVTLWKRNGIKVAGRVIADITQACVVTLDPVQAHIDEAVDGLFLPEAS
                     KLGRQGFGDGGEIMIDADGPDSPEVFSGDSVDVGGLAEQFFGLAIDPYPRKGGVDLAA
                     AKVVDGDEPVENEFQRKLRLLLGKS"
     gene            complement(6081..6623)
                     /locus_tag="VGM46_01800"
     CDS             complement(6081..6623)
                     /locus_tag="VGM46_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome C chaperone family protein"
                     /protein_id="HEY4191354.1"
                     /translation="MFKRLFGRERHANRAITEALYGEIVAAARQSVFYSHWNVPDTPL
                     GRFEMLSLHMFLFQHRLHGEQGVSQLLAQVLIDEFFLDVEHSLRELGISDVGVPKRMK
                     KLAKMFYGRTAAYADSLERGDGASLAAALTRNVTPEATEWPQAQNLAAYALRVEKDLA
                     LQPSEAICAGTLSFPIAEAV"
     gene            6888..7352
                     /locus_tag="VGM46_01805"
     CDS             6888..7352
                     /locus_tag="VGM46_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006332635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEY4191355.1"
                     /translation="MLAVVSVLSACNTSKTFGDLSPGETLNQGYIVDQESLDLVPVGS
                     SREQVVLALGSPSTTATFDNEVFYYISQTRRRAVAFAKPKLVDQKVLAVYFGPDGRVA
                     SIANYGLKDGKVFDFISRTTPTGGKDQNFLMQVIKGAGKMAPSVPSGLNQGM"
     gene            complement(7454..>8479)
                     /locus_tag="VGM46_01810"
     CDS             complement(7454..>8479)
                     /locus_tag="VGM46_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018427368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HEY4191356.1"
                     /translation="HGTNVIQGLAVSLESTALPAIVIVGGIIGTYQLAGLFGTAIAVT
                     TMLGLAGMIVALDAFGPVTDNAGGIAEMAGLPKEVRHSTDALDAVGNTTKAVTKGYAI
                     GSAGLGALVLFAAYSNDLKFFAANGDKFPYFQGMGEVSFDLSNPYVVAGLIFGGLIPY
                     LFGGIAMTAVGRAAGSIVEEVRKQFREDPGIMAGTSKPNYARAVDILTKAAIKEMIIP
                     SLLPVLAPLVVYFGVLLISGSKASAFAALGASLLGVIVNGLFVAISMTSGGGAWDNAK
                     KSFEDGFTDKDGVKHLKGSEAHKASVTGDTVGDPYKDTAGPAVNPAIKITNIVALLLL
                     AVLAHAA"
BASE COUNT         1576 a         2633 c         2606 g         1664 t
ORIGIN      
        1 gacagccggt tggcgacgac gcaaccggcg gaccccgcgc cgacgacgat atagtcgtag
       61 ctgccgacgt cttgagtgtc ttgcatcatg gggctgtcct gcgttgccgg taaaaagcgc
      121 ctggaatcgg tttagcttca agctttaccc ggccgccgtc aaacccttcg tcgaaagctg
      181 ccttgttgcg ctggatgacg gccggctttg acagggccgc ccaaatccac gcccgacaat
      241 cgggctttgt gccattcccc ggattttagt gcttgcccta aaacgccgga gaatataggg
      301 ctttgcgcgg tccggagtgt agcgcagccc ggtagcgcac ttgactgggg gtcaaggggt
      361 cgtcggttcg aatccggcca ctccgaccat ttgaagccaa ggacttgccc ccgaagtcca
      421 atccagaaaa tctcgcatcg agcaaaataa gagggttcct cggcgcaggt tgcgttgggg
      481 tcttcaccgc tgcgattcgc tgccgaacgg cacatccgtc ggcctgacca acggctcgcg
      541 aaatgagccg ctaccgcacg ggatcgacga tctttgcgcg tgaacggaaa cccggcggcc
      601 gcgtcaggcg cgtgaccgcc ggtatggcga cggacaaaaa taacccgcgc tgaacgcagg
      661 aaccgtttgt gccaccgatg gcgaaaggcg gcctgtgatg gccgccttga agtctacttt
      721 gtcagaaacg gaagtccgcc ctttcagaaa acgacggtct tgtggccgtt cagcatgacg
      781 cggccttcga cgtgcttctt cacggcgcgg gcgagaacgc ggctttcgac atcgcggccc
      841 gccgctacga aatcctcggc ggtcatggcg tgggagacgc gctccgtctc ctgctcgacg
      901 atcgggcctt cgtcgaggtc cgccgtcaca tagtgcgcag tggcgccgat cagcttcacg
      961 ccacgctcat gggcctgatg atagggcttg gcgcccttga acgatggcag gaacgagtgg
     1021 tggatgttga tgatcttgcc atatagccgc ttcgacacat tgtccgacag gacctgcatg
     1081 taacgggcga ggacgacgag atcggcgtcg gtcttgcgca cgagatccag aaggttctcg
     1141 tccagctccg gcttgttgtc cttggtggcc ttccagaaat gatagggaac gccttcgagt
     1201 tcggcggtgt tgcggctgtc ctcgtggttg gagacgatgg ccaccacctc ggcgttcagc
     1261 cagccgacct tcacctggta gagcagatgc agcagcgcat ggtcgaactt cgacaccatg
     1321 atgatgatgc gcggcttgcg cgtgcggtcg gtgagcgtcg tcttgatgcc gaagcggtcc
     1381 acggcgggct tgatcgaacg ggtgatgtcg tcgctggttt tctgcgaatc ggccgtaaag
     1441 gcgatgcgca tgaaaaactg accggtctgg tggtcccaat actggttgct ttcgacgatg
     1501 tttgcgccaa tcgaggccag ttccgtggtc accgcggcaa cgatgccgga ctggtcgacg
     1561 caattaagag taaggatgaa tgagcgatcg ttcattgtgt actcgttcga ttcgggttcg
     1621 aggatcggtt gcggctttcc gcctttcagc ttcttattgg cgagccgtac accattgccg
     1681 gcaggcccag ccatatcgag gacgacattt gacgccgctt gcgtttgcat ttcgcaaaca
     1741 tatgccgctg cgaaagaccg aatggcagcg accgggcgcc attgcctgcc ggcaacgccg
     1801 aaagctgcca aacgacaaaa aacgccgcca tggagtggcg gcgccggtcg atcacggatt
     1861 tttatgagcg ggtacccgcc tagcggcggt tgaacagcat tgccgccacc aagcctacca
     1921 ccaccaggcc gaccgctgcg gcggtcgccg gatggtcgcg cgctgtgcct tcaatgacct
     1981 tggcgcgctt gcgcaatccg ggcagcgcgt cgctgatgcg ttcggccaaa tccgaatagc
     2041 tgtcccatgc ggcatcccgg ccctcgtcat aataggcctt gccgcgcttc gacgccgttt
     2101 tcttcaacgc cgccaattcc ttggacaacg aagctatctg cttttcgagg tcgatatcag
     2161 tcatatcgga gaacgatggc atggcaattt ccttcttcgc aagaagaaag cctaacggcc
     2221 tcagcgctct atcgttccat gccggtcgtc agcggacctg atcgagcagg cgcttgcatt
     2281 cgagcaggtc gaacagcgct tcctgcaaca gcgcgcggtt gtcgcgcgac agcttggacg
     2341 catccgaggg cacggggcct tcgtcgtcgc ccttgccgag gccgaagaag cggcggctgg
     2401 gctttaccct cggctgcccg accatcatct catcgtccgg gccgcgaggc tgcgctgccg
     2461 gcgtgagcgg cacggcgtcg gcctgcttgc gctgggcaat cgcctcgacg gcggcaatat
     2521 cgccgctgcc tatggcaacc aggaactgat tgccattttc gcgcagcagt ttctgcacgc
     2581 ccttgatcgt gtaaccctgg tcgtagagca ttgcgcggat gcccttgata agctcgacat
     2641 cctgcggccg gtaatagcgg cggccgccgc cccgcttcat cggcttgatc tgggtgaagc
     2701 gcgtttccca gaaacgcagg acatgctgcg gcagatcgag gtcttcggcg acctcgctga
     2761 tggtgcggaa ggcatcgggg ctcttgtcca tgagcgaaat cctcacgctg gacgacgatc
     2821 cggcatcata tggccgaatc ggtctccatt tcagcggttt atgctgcaac attcaagccc
     2881 ggcacatggc ggcggaccat tttccgctcg cgccaatgcg ctcggcgggt cgcgttattt
     2941 cgcggctttg gccttgctgc tctggtggga gcgcaatatc cggctcttca agacgttcga
     3001 cgccttgaag gtcatcacgc ggcgcggcag gatcggcact tcctcgccgg tcttggggtt
     3061 gcggccaatg cgttcgtttt tcgagcggac gtggaatgtc gcgaaggacg acagcttcac
     3121 cgtctcgccg cgcacgatcg cctcgcaaat ctcgtccagt acggactcta cgagttcggc
     3181 ggattccgtg cgcgacaaac cgaccttccg gtacacggct tcagctaggt cggcgcgcgt
     3241 aagtgtcttt ccccccatgc gagcgtccca tctgcttaaa aataaaattg tccaggcaat
     3301 gccggagcct aagtaattga tccggcaagg tcaaggaccg aacccgaggg ttcttgcaac
     3361 cttaccagcg caccagcacc gcaccccagg tgaagccacc gcccatcgct tcaagcagga
     3421 cgagatcgcc cttcttgatg cggccgtcgg caacggcgac cgagagcgcc agcggcacgg
     3481 acgccgccga tgtattaccg tgcagatcga ccgtcacaac cactttttcg tcggcaatcc
     3541 caagcttctt ggccgaagcg tcaataattc gtttattcgc ctgatgcggc acgaaccagt
     3601 cgaggtcctc ggcggtaatg ccggcttcag tgaacgtcgc ctcgatgaca tcggtgatca
     3661 tgcccacggc gtgcttgaac acctcgcggc cttccatcct caactggccg accgttcccg
     3721 tggtcgaagg cccgccatcg acgtagagct tttctttgtg gacgccatcg gagcgcaggc
     3781 ttgccgccag aacgccacgg tcggaaatat cgccggcgcc ctcgcccgcc tccagcacga
     3841 ccgcgcccgc gccatcgccg aagagcacgc aggtcgaacg gtcgctccag tcgaggatgc
     3901 gcgaaaaggt ttccgagccg atcaccagcg cgcgctttgc caggccgccg cgaatataga
     3961 gatcggcagt ggtgatcgca tagacgaagc ccgaacacac cgcctgcatg tcgaaggcga
     4021 agccattatg catgccgagc cggttctgga tttcgacggc ggtggccgga aacgtgttgt
     4081 tgggtgtcga ggtcgccagc acgatcaggt cgatgtcgga cgccaccagc ccggcagcgt
     4141 ccagcgccgc gcgcgccgcc gcctcgccca gcgaagccgt cgtctcgtca tcggcggcga
     4201 tgtggcgctg gcggatgccg gtgcgctgcg cgatccattc gtcggaggtt gcgaccatgc
     4261 cttcgaaatc ggcattcttc atgatgcggc gaggcaacga ggcacctatg ccgcgcacaa
     4321 gcgatctgat caaaggtgta tcctattcct gttcttcggt gtcgtcgctg gaccgtagcg
     4381 atgcagcgtg gggattgcgt gcgtggaaaa gatcgaggtc ggcttcgatg cggtccagca
     4441 ggcggttgcg caccatgtcg tagccgagtt cgacggcggc ggcgaaaccg ttcgaatcgg
     4501 cgccgccatg gctttttacg acgatgccgt tgaggccaag gaacacgccg ccattggagc
     4561 gcccgacatc gagtttttcc ctcagccggt tgaacgcgcc cctggcgaaa atatagccga
     4621 tacgcgccat cagcgtccgg ctcatcgcct cgcgcagata ttgggcgaac tggcgggcgg
     4681 ttccctcggc ggtctttagc gcgatgttgc cggaaaaccc ttccgtcacc accacgtcga
     4741 ccgcgccctt gccgatatcg ttgccttcaa cgaagcccac ataattcagg gaagccatat
     4801 tggcgtcgcg cagcatgcgc cccgcctcct tgacctcctc ctggcccttg atctcctcga
     4861 cgccgacatt gagcatgccg acagtcggcc ggtcgatgcc gaacaccgag cgcgccatgc
     4921 cggtgcccaa tatggcgaag tcgatgagtt ggtgggcatc cgcgccgatg gtggcgccaa
     4981 cgtccagcac cacgctttcg ccgcgcaaag tcggccagat ggcggcaatg gccgggcggt
     5041 cgatggtcgc catggtgcgc aggcagaatt tggacatcgc catcagcgcg ccggtattgc
     5101 cggccgaaat gcaggcatcc gccgtgccgt tcttcaccgc ctcgatcgct ttccacatcg
     5161 aggatttcca gcggccgttg cgcagcgcgg cacttggctt gtcgtccatc ctgacggcga
     5221 cttcgcagtg gataagctcg cttacttcga ccagcttggg gtacctggcc agaatcggcc
     5281 gcacgatttc ctcgcggcca tagatgacaa agcgaatgtc ggtgcgccgc gttgcgaccg
     5341 tcataagtgc cggtatgacc acgtccggcc cgtgatcgcc gcccatggca tcgatggaaa
     5401 tcctgatcac tcgatattca tctcacggtt tgccccgggc tcgcggcggt ttggcgaaaa
     5461 tagcggtttt gcgcctgcgc acaaccggct ttatgtccat tggaccggct ttcaggattt
     5521 tccaagcagc aggcgcagct ttctctggaa ttcgttttcg acaggctcgt caccgtcgac
     5581 gactttggcg gcggcaagat cgacgccgcc cttgcgggga taaggatcga tcgccaggcc
     5641 gaagaattgt tcggccaatc cgccgacatc cacgctgtca ccggaaaaaa cctccgggct
     5701 gtccgggccg tcggcgtcga tcatgatttc gccgccatcg ccgaagcctt ggcgcccaag
     5761 cttggacgcc tcgggcagga acaggccgtc aaccgcttca tcgatatggg cttgcaccgg
     5821 atcgagcgtg acgacgcaag cctgcgtgat atcggcgatg acccggccgg cgaccttgat
     5881 gccgtttcgc ttccagagcg tgacgtccag ttcggcccgc cagttttcca ccgacagcag
     5941 cccgtgctct tcggcaagtg ctgctttctg ggccgaatcg gcctcgaccg tcacttgcag
     6001 gcccttttgc ggcagccgcg cgacgtggac actgaaggaa accgggcttt tggggctagt
     6061 ctcgctcatc agccttcctt tcagaccgct tccgctatcg gaaaggaaag cgttccggcg
     6121 caaatagcct cggatggctg caaggcaagg tctttttcga cgcgcagcgc ataggcggca
     6181 aggttctgcg cctgcggcca ttcggtcgct tccggcgtca cattgcgggt aagggcggcc
     6241 gcgagcgacg cgccatcgcc gcgttccagc gaatcggcat aggcggcggt gcggccataa
     6301 aacatcttgg ccagcttctt catgcgcttg ggcacgccga catcgctgat gcccagttcg
     6361 cgcagcgaat gctccacatc caggaagaac tcatcgatca gcacctgcgc aagcagttgc
     6421 gagacgccct gctcgccatg cagccggtgc tgaaacagga acatgtgcag cgaaagcatc
     6481 tcaaaccggc caagcggcgt atccggcacg ttccagtgcg aataaaagac gctttgccgc
     6541 gccgccgcca cgatttcccc gtagagcgcc tcggtaatcg cacggttggc atggcgttcg
     6601 cggccaaaca ggcgcttgaa catagaggga agctctcccg aaagttgatc atcggcccga
     6661 aggttggttt ctggcctgaa agttgatagc tggtccgagg gtggtttgtt ggggtggcgt
     6721 tgttgcatcg gcagccaagc tggtttaccg gttttgcggc gccgtgcaag ccggccgatc
     6781 taatggagtt gttgttgcgc gcgcaaaagg tcaagccaat ttcttcgcca aagcccattt
     6841 cttcgccaag gtcggtattt tcggcaatcc caaccgggat cgtgtcgatg ctggcggtgg
     6901 tttccgtgct gtctgcctgc aacacgtcga agacgttcgg cgaccttagc cccggcgaaa
     6961 ccctcaacca gggctacatc gtcgaccagg aatcgctcga cctcgtgcct gtcggctcca
     7021 gccgcgagca ggtggtgctg gcgcttggca gcccgtcaac cacggcgacc ttcgacaacg
     7081 aggtcttcta ttacatttca cagacgcgcc gccgcgccgt cgccttcgcc aagccgaagc
     7141 ttgtcgacca gaaggtgctc gccgtttatt tcggcccgga cgggcgcgtc gccagcatcg
     7201 ccaattacgg cttgaaggac ggcaaggtgt tcgacttcat ctcgcgcacc acgccgaccg
     7261 gcggcaagga ccagaacttc ttgatgcagg tcatcaaggg cgccggcaag atggccccca
     7321 gcgtgccaag cggcttgaac cagggcatgt agcgcaaggc gctttaccgt cgtcaggcag
     7381 ttttcgcatt caccaagcaa aaaacaaaaa cccgcgggat cgctcccgcg ggttttcttg
     7441 tcgcttgacc ggatcaggca gcgtgggcca gcaccgccag cagcagcaag gccacgatat
     7501 tggtgatctt gattgccgga ttgaccgccg ggccagccgt atccttgtag gggtcgccga
     7561 ccgtatcgcc ggtgaccgag gccttgtgcg cttcggaacc cttcaggtgc ttgacgccgt
     7621 ccttgtcggt gaagccgtcc tcgaacgact tcttggcatt gtcccacgca ccgccgccgg
     7681 aggtcatcga gatggcgacg aagaggccgt tgacgatgac gccgagcagc gaagcgccaa
     7741 gcgcggcaaa ggccgaggcc tttgagccgg agatcagcag cacgccgaaa tagacgacga
     7801 gcggcgccag caccggcagc agcgagggaa tgatcatttc cttgatcgcc gccttggtca
     7861 ggatatctac cgcgcgcgca taattcggct tggaggtgcc ggccatgatg ccggggtcct
     7921 cgcggaactg cttgcgcact tcttccacga tggaaccggc agcccggccc accgccgtca
     7981 tggcgatgcc gccgaacagg tagggaatga ggccgccgaa gatcaggccg gcgacgacgt
     8041 aagggttgga gaggtcgaac gaaacttcgc ccatgccctg gaagtagggg aacttgtcgc
     8101 cattggcggc aaagaacttc aggtcgttcg aataggcggc gaacagcacc agcgcgccaa
     8161 ggccggcgga accgatggcg tagcccttgg tgaccgcctt ggtggtgttg ccgaccgcat
     8221 cgagcgcgtc ggtggagtgg cgcacttcct tgggaaggcc ggccatttcc gcgatgccgc
     8281 cggcattgtc cgttaccggg ccgaaggcgt cgagcgccac gatcatgccg gcgaggccga
     8341 gcatggtggt gacggcgatg gccgtgccga acaggccggc cagctggtag gtgccgatga
     8401 tgccgccgac gatgacgatg gccggcaacg ccgtcgattc cagcgaaacc gcaaggccct
     8461 ggatgacgtt ggtgccgtg
//
LOCUS       DASYCS010000045         2939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4357379,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000045 DASYCS010000000
VERSION     DASYCS010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4357379"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1260)
                     /locus_tag="VGM46_01815"
     CDS             complement(<1..1260)
                     /locus_tag="VGM46_01815"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019859096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEY4191357.1"
                     /translation="MKLKELAGIVPIEGIAPSDTVVTGISSDSRQVAPGTLFFALAGS
                     KADGSAYAADAASRGAVAVVGASGSAVAGLSIPFFSVADPRLALALSAARFFRAQPET
                     MVAITGTAGKTSVAAFTRQIWERAGLQAASIGTTGVVAPGRNEYGSLTTPDPVALHRL
                     LAELAQAGVTHASMEASSHGLDQRRLDGVRLKAGGFTNLGRDHMDYHPTMEEYHRAKL
                     RLFDTLLPKGAPAVIFADDAWSEPTVKAALAAGLDVLTVGRHGSFLKLKRVEHERSRQ
                     RAEVEADGVLYEIDLPLAGDFQIANALVSAGLAISTGTPVATAIAALEKLKGAPGRLD
                     LIGTTANGAPVYVDYAHKPDALENVLSAVRPFTTGKVVVVFGCGGDRDRGKRPIMGEI
                     ATRLADVTIVTDDNPRSEAPETIRAAIL"
     gene            complement(1322..>2939)
                     /locus_tag="VGM46_01820"
     CDS             complement(1322..>2939)
                     /locus_tag="VGM46_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006204207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEY4191358.1"
                     /translation="KTRVVMTMAVFFGIYTMIAGRLVYLGYQDPDNSGAPASRVTASR
                     PDIVDRNGELLATDIKTASLYAEPRHIVDADEAIEKLSTVLPEIDYEQTYHKLKSGAG
                     FVWLQRQLTPKQQNDILQLGIPGIGFRTEKRRFYPSGDTSTYVVGMTNIDNQGISGME
                     KYIDSQGLSDLQASGLAMPRDLAPVKLSIDLRVQHIAHDEIVSGMERFHAIAAGAVVL
                     NVKTGEVIAMASVPDYDPNNPYNAQDKNRLNRMSAGLYEMGSTFKSFTSAMALDSGKA
                     TMNSRFDASHPIRVGHQAIHDFHGKNRMLSLPEVFLYSSNIGSAREAELVGIDAHREF
                     LHRVGILEKMQTELPEVARPTEPKVWKQVNSFTIAFGHGVSTTPLQAAVGCAALINGG
                     YLMEPTFLVRSAESAMATAKKVVNEKTVEGIRYLYTLNAEKGSARNARVPGYRVGGKT
                     GTAEKVINGRYSKDLNFNTFVAAFPMDDPQYLVFTIADAPRPEKPGMGATAASNAGII
                     AHNIISRSAAMLGVKPDFSHENGATLVSSE"
BASE COUNT          532 a          913 c          925 g          569 t
ORIGIN      
        1 caatatggcg gctcggatcg tctccggcgc ttccgaacgc ggattgtcgt cggtgacgat
       61 ggtgacatcc gcaagccgcg ttgcaatctc acccatgatc ggtcgcttgc ccctgtcgcg
      121 gtcgccaccg cagccgaaca ccacgacgac cttgccggta gtgaagggcc gcacggcgct
      181 cagcacattc tccaaagcat ccggcttatg cgcataatcg acatagaccg gcgcgccatt
      241 ggcggtggtg ccgatcagat cgagccgccc cggcgcgcct ttcagcttct ccagcgccgc
      301 aatcgcggtg gcgacgggcg ttcccgtgga aatggcaagc ccggccgaaa ccagcgcatt
      361 ggcaatttga aaatcgccgg ccagcggcag gtcgatctcg taaagcacac cgtccgcttc
      421 aacttcggcg cgctggcgac tacgctcatg ctcgacgcgc ttgagcttga ggaaagagcc
      481 atgccgccca accgtcagca cgtccagccc ggcagctagc gccgccttga ccgtcggttc
      541 cgaccacgca tcgtcggcga agatgacggc cggcgcgccc ttgggcagca gcgtatcgaa
      601 cagccgcagc ttggcgcggt gatattcctc catggtcgga tggtaatcca tgtggtcgcg
      661 gccgagattg gtgaagccgc cggccttgag ccggacgcca tcgagccgcc gctggtcgag
      721 gccatggctg gatgcctcca tcgaggcatg cgtgacgccc gcctgcgcca gttcggcaag
      781 cagccggtgc agcgccaccg gatcgggcgt ggtcagtgag ccatattcgt tgcggccggg
      841 cgcaaccacg ccggtcgtgc cgatcgaagc cgcttgcagc ccggcgcgct cccaaatctg
      901 ccgggtgaag gccgccacgg aagttttgcc cgccgtgccc gttatggcca ccatcgtttc
      961 cggctgtgca cggaagaatc gggcagcgct cagcgccagc gccaggcgcg gatcggcaac
     1021 ggagaaaaac gggatcgaaa gcccggcgac cgcgctgccg ctggcgccga cgaccgccac
     1081 cgcgccgcgg ctggcggcgt cggccgcata ggccgagcca tccgccttgc tgccggccag
     1141 cgcaaaaaac agcgttcccg gcgcgacctg ccgggaatcg gaggaaatgc cggtgaccac
     1201 tgtgtcagaa ggcgcaatgc cttcgatggg cacgataccg gcaagttctt tgagcttcat
     1261 ggaaatccgt tcgtcccacc gtttgcaaaa cgacgcgccg ctaacggcgc gtcgacgaga
     1321 atcactcgga agaaaccagt gttgcaccgt tttcatggct gaaatccggc tttacgccaa
     1381 gcatggccgc cgaacgcgag atgatgttat gggcgatgat gccggcgttc gacgcggcgg
     1441 tggcgcccat tccgggcttt tccgggcgcg gcgcgtcggc aatcgtgaac accagatatt
     1501 gtggatcgtc catcggaaag gcggcaacga aggtgttgaa attcaaatcc ttcgaataac
     1561 ggccgttgat gactttttcg gccgttccgg tcttgccgcc gacgcggtag ccgggaaccc
     1621 tggcgtttct agccgagcct ttctcggcgt tgagcgtata gaggtagcgt atgccttcga
     1681 cggtcttttc gttgaccact ttcttggcgg tagccattgc actctctgcg ctgcgcacga
     1741 ggaatgtcgg ctccatcagg tagccgccat tgataagtgc tgcgcagccc acggctgctt
     1801 gcagcggcgt cgtcgacacg ccgtggccaa aggcgatggt gaaggaattt acctgcttcc
     1861 agaccttcgg ctcggtcggg cgggcgactt caggcagctc ggtctgcatc ttttcaagga
     1921 tgccgacacg gtgcaggaat tcccggtgag cgtcgatgcc gaccagctcg gcttccctcg
     1981 ccgaaccgat attggatgaa tagaggaaca cttccggcag tgacagcatc ctgttcttgc
     2041 cgtggaaatc gtgaatggcc tggtggccga cccggattgg atgcgaggcg tcgaaacggc
     2101 tgttcatcgt cgccttgccg gaatcgagcg ccatggccga ggtgaagctc ttgaaggtag
     2161 accccatttc gtagaggccc gccgacatgc ggttcagcct gttcttgtct tgtgcattgt
     2221 atggattatt cggatcgtag tccggcaccg aggccatggc gatcacttcg ccggtcttga
     2281 cgttgagaac gaccgcgccg gcggcaatgg cgtggaaccg ctccatcccc gacacgatct
     2341 cgtcatgcgc gatgtgctgg acgcgcaggt caatcgacag tttcaccggc gccaggtccc
     2401 tgggcatggc caggccggac gcctgaagat cgctcaggcc ctggctgtcg atgtatttct
     2461 ccatgccaga gatgccctga ttatcgatat tggtcatgcc gacgacatag gtcgaggtat
     2521 cgccgctggg atagaagcgg cgcttttcgg tgcgaaagcc gatacccgga atgcccagtt
     2581 gcagaatgtc gttctgctgc ttcggcgtca gctggcgttg cagccagacg aagccggcgc
     2641 cgcttttcag cttgtggtag gtctgctcgt agtcgatctc cggcaacacc gtcgacagtt
     2701 tttcgatggc ctcgtcggca tccacgatgt ggcgcggctc ggcatagagc gaggccgtct
     2761 tgatgtcggt ggccagcaat tcgccgttgc ggtcgacgat atcgggccgc gatgccgtca
     2821 cccggctggc cggcgcgccg gaattgtccg ggtcctgata gccgagatag accagccggc
     2881 cggcaatcat cgtgtagata ccgaaaaaca cggccatcgt catcactacg cgcgtcttg
//
LOCUS       DASYCS010000046         8244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4501791,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000046 DASYCS010000000
VERSION     DASYCS010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 8244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8244
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4501791"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="VGM46_01825"
     CDS             <1..403
                     /locus_tag="VGM46_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191359.1"
                     /translation="FSMPFCMAVGFFDGDAGLEQFTDEKARDPKILELASKISYVIDP
                     ENEYPRNYTGHIRVTLADGSVRELRQPHMRGGVREPLTREQLIAKFRGNVSYGGASAT
                     FGDRLLDFCLNLEDCSDMRAFGVFRDLADGV"
     gene            591..1979
                     /locus_tag="VGM46_01830"
     CDS             591..1979
                     /locus_tag="VGM46_01830"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY4191360.1"
                     /translation="MTGAVEPSISEQLADWLVGIDLAAIPGRARDAALDTTIDVVGLC
                     VAARDNDYTKAALAAWNGAGPCTVFGIGETRDPYSAALINGTAAHGEDFDNTFEGCPV
                     HSGAVIVPAILAMAQSEGIDGARTLSSLAVGIELMCRLGLVARKGIHAAGFHPTAVLG
                     TVSAAAAAGVMLGLNRRQFVDALGVAGSMASGIIEYLSDGSWTKRMHPGWAAQAGMRA
                     ARMGQAGFRGPRTMFEGEHGLFRSFAPTLTPDFSQLVDRLGSSWQMENVAFKPYACGT
                     MTQPYIDCAIALRNDGVRPQDIVDIVCETADGTVHRLWEPLQLKQRPPNGYAAKFSTP
                     YCVAVGLIDGEGGLQQFTDERVADPEIAALAGRVRYEIDPDNEYPLNYTGHLRALLSD
                     GRVVEKRQPTLRGGTRQPMGREELIAKYRKNALYGGVGEAKASAMLDAILAIGTTGSP
                     AELGEAARRKDV"
     gene            1979..2734
                     /locus_tag="VGM46_01835"
     CDS             1979..2734
                     /locus_tag="VGM46_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007753509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY4191361.1"
                     /translation="MDKRELAGKTALVTGAARNIGRSIAVALAKRGANIVVCALSSAN
                     EAAETAALAEAEGVQAMVCLGDVTSEADVQRIVAQACERFGGIDFLVNNAAVRREAKI
                     EDVTLEQWHSLLAVMLDGPFLMSKACIASMRQRGSGRIVNIGGMTGHTGAPERAATVT
                     GKAGLAGLTRALAHDLAGDGVTVNCVVPGMIDTVRGGSNALAPAHHATRNLLLGRRGR
                     PEEVADLVAYLCGPLAGFVTGQSIHVNGGTFMA"
     gene            2890..3636
                     /locus_tag="VGM46_01840"
     CDS             2890..3636
                     /locus_tag="VGM46_01840"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY4191362.1"
                     /translation="MTPASASMVHQPELKPLYLQIQEMLIERVVNGEWGPGEMLPSEM
                     RLAVEYNVHQGTVRKALDAMAMRNLIVRHQGKGTFVASVSMRHSPFRYFKLVFLNNPA
                     ERPVSHFISITVAQASKQEQSALQLAGPEDKVVRATKVRAYGAVPAIMEEIAYPEGMF
                     PGLADLFRKLHPDTTYGLLEQKYRVLITTVKDQIGSAAATSLDAEHLKVAIGEPLLRI
                     NRTAFAVDGRPAEWRVSRCTNACEYVLDQM"
     gene            3885..4826
                     /locus_tag="VGM46_01845"
     CDS             3885..4826
                     /locus_tag="VGM46_01845"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY4191363.1"
                     /translation="MGPLLWAVPTFAQENKYDYPVTTVTLTTHSKPGAGSDLFLRKLS
                     AALSKEMGINFVVDYWTGGSGAKAMAELAKAKPDGSVFYATTPTHINTSLLSEPPVTY
                     KDIDYIANVFFDPEIIYTLSSSPFKTMKDAVDAAKASPGSQIWGGSTPGSLERQILER
                     INQHTGAGAVVVPHDDGAGLLIDVLNGTVSLGVGELQEVQSYLQAGKMKVLAVYYPQR
                     LEMLPDVPTIQELGLDTETVRKFRGLAGPKGIPPETVKKIDTAIEAILKDKDFIKIYS
                     EDNLVPGYMNQEDYRAFMVKFVEAEKGFIKDFGIKND"
     gene            5164..6192
                     /locus_tag="VGM46_01850"
     CDS             5164..6192
                     /locus_tag="VGM46_01850"
                     /inference="COORDINATES: protein motif:HMM:NF015558.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HEY4191364.1"
                     /translation="MTITATAPQSRADNALAAYVPGLVLVAVITAAAYGVRHLPFMSP
                     LSPMILAIFVGMLFANVVGSNISAAEGISISGKRLLRLAVALLGLQISFAQLAEIGVR
                     GVGLAAIGLFSTFLFTIWMGKLLGVDRNVARLLAAGTAVCGASAIAAANAVTGADDED
                     TSYAVACITLFGTIAMFLFPFLNFVAGLSSHAYGLWVGLSVHEVAQVVGAGFQGGKEA
                     GEIAVITKLSRIMMLAPLIIGMSFVLSRSSSRASDKKGQVPLVPFFVVGFLVLAALNS
                     FGLVPEAIRSPIVTATPVLLTAAMAALGLGTSVSKLKQHGLRPFLLAGMATVFIAGVG
                     FLMVTLVA"
     gene            6274..7164
                     /locus_tag="VGM46_01855"
     CDS             6274..7164
                     /locus_tag="VGM46_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007568968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY4191365.1"
                     /translation="MTFDQLRIFLAVAEREHVTRAADFLNLTQSTVSGAITALEARHN
                     VKLFHRVGRRIELTDEGRIFADEARAILAAVRSAETTLADLGGAPRGALSIFASQTIT
                     SYWLPQYLVGFHKLYPQIDLHLEAGNTAQCVRAVNAGSADIGFIEGTINEPGVCTRLV
                     ARDRLVIVVGADHPWAKKPPQSPLELVEQQWVLREKGSGTRSSFEEALSAHAIQPSQL
                     RVAIELPSNEGICTAVATGQYPTAVSETVAEAGVNSGRLVTVPFELPSRAFSLILHRE
                     RHQSRACKAFCEMLPARESL"
     gene            complement(7218..7293)
                     /locus_tag="VGM46_01860"
     tRNA            complement(7218..7293)
                     /locus_tag="VGM46_01860"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7258..7260),aa:Ala,seq:cgc)
     gene            7498..7791
                     /locus_tag="VGM46_01865"
     CDS             7498..7791
                     /locus_tag="VGM46_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006327350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEY4191366.1"
                     /translation="MNALIDTLIYALSLYWWIIIASAIFSWLFAFNVVNSRNQFVNNI
                     GTVLYRLTEPVYRPIRRYLPDLGGIDISPIIVLLIIFFLQRFLDTTVRAAFYY"
     gene            complement(7818..>8244)
                     /locus_tag="VGM46_01870"
     CDS             complement(7818..>8244)
                     /locus_tag="VGM46_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEY4191367.1"
                     /translation="RGQPIPTGWAQDGSGAPTTDAGEGLRGSLLPSGGQKGANLALMI
                     EILATALTGAKLSSQASMFGDDQGGPPGVGQFTLAIDPARFSARFAESLGALAESFEA
                     AGVRLPGSGATAGKQDRDSILIDVDADLWQRCLDLAQGR"
BASE COUNT         1525 a         2505 c         2591 g         1623 t
ORIGIN      
        1 gttcagcatg ccgttctgca tggcggtcgg tttcttcgac ggcgacgccg gtctggagca
       61 gtttaccgac gagaaagcca gggacccgaa aatactggag ctggcatcca agatttccta
      121 cgtcatcgac ccggaaaacg agtatccacg caactacacc ggccatatac gggtgacttt
      181 ggccgatggt tcggtgcgcg aattgcgcca gccgcatatg cgcggcggcg tcagggagcc
      241 gctgacgcgc gagcagttga tcgcaaagtt ccgcggcaat gtcagctatg gcggggcaag
      301 cgccacgttt ggcgaccgcc tgctcgactt ctgcctcaat ctcgaagact gctccgacat
      361 gcgggccttt ggcgtgttcc gggatctcgc cgacggggtg tgaactcaat tgaccggctg
      421 gcgtctggct gccggtttcc agatatgctg cccagatgcg cgtcccgaat acgccgtcca
      481 aaatacacag taaggagtgg caaagtgcaa ttcagactga ccgatgaagc cgttccagcc
      541 gtgaaggcag gccctgagct ttgcgcattc ttcgccaaag ttgtgcggcc atgacggggg
      601 cggttgaacc cagcatttcg gagcaactgg ccgattggct ggtcggcatc gatctcgccg
      661 ccattccggg ccgtgcccgc gatgccgcgc tggacacgac gatcgatgtg gtcggccttt
      721 gcgtcgcggc aagggacaat gattatacga aggcggcgtt ggcggcatgg aacggtgctg
      781 gcccctgcac ggtattcggc atcggcgaaa cgcgtgatcc ttatagtgct gcgctcatca
      841 acggcaccgc cgcgcatggc gaggatttcg acaacacctt cgaaggttgt ccggtccatt
      901 cgggagcggt gatcgttccg gccattcttg cgatggcgca gagcgagggg atagatggcg
      961 cgcgcacgct aagctcactt gccgtcggca tcgaactgat gtgccggctc ggccttgtcg
     1021 cgcgcaaggg cattcatgcc gccggctttc atccgaccgc cgttttgggc accgtttcgg
     1081 cggcggcggc ggccggcgtg atgcttggcc tgaaccgcag gcagttcgtc gatgcgctgg
     1141 gggtggccgg cagcatggcg tcgggcatca tcgaatatct ttccgatggc tcatggacca
     1201 agcggatgca ccccggctgg gcggcgcaag ccggaatgcg ggcggcgcgg atggggcagg
     1261 ccggttttcg cgggccgcga accatgttcg aaggcgagca tggcctgttc agatcgttcg
     1321 cgcccaccct gacgcccgat ttcagccagc ttgtcgaccg gttggggtct agttggcaga
     1381 tggagaatgt cgcctttaag ccctatgcgt gcggaacgat gacccagcca tacatcgact
     1441 gcgcgatcgc gcttcgcaac gatggcgtgc gtccgcagga cattgtcgac atcgtctgcg
     1501 agaccgcgga cggcacggtc caccggctat gggagccgtt gcagctgaag cagcggccgc
     1561 cgaacggcta tgcggcgaag ttcagcacgc cctattgcgt cgccgtcggg ttgatcgacg
     1621 gggagggcgg cttgcagcaa ttcaccgacg agcgcgtcgc cgacccggag attgcagcgc
     1681 ttgccggccg cgtccgctac gagatcgacc ccgacaacga ataccccctc aactataccg
     1741 gccatctgcg cgcgctgctc agcgacgggc gcgtcgtcga aaagcgccag cccacgctgc
     1801 gcggcggcac gcgccagccg atgggaaggg aggaactcat tgccaaatat cgcaagaatg
     1861 ccctctatgg cggcgtcggc gaggctaagg cgagcgccat gctggacgcc atcctggcga
     1921 taggcacaac cggcagtccc gccgaactag gcgaggcggc ccgtcgaaag gacgtctgat
     1981 ggataagcgc gaactcgccg gaaagacggc gctggtcacc ggggcggccc gcaacatcgg
     2041 ccgctcgata gcggtggctt tggcaaagcg gggcgccaat atcgtcgttt gcgcgctaag
     2101 ctcggcaaac gaagccgccg aaaccgccgc gctcgccgaa gccgagggcg tgcaggcgat
     2161 ggtttgcctt ggcgatgtca ccagcgaggc cgatgtgcag cggattgtcg cgcaggcctg
     2221 cgaacgcttt ggcggcatcg atttcctcgt caataacgcc gccgttcgcc gtgaggcaaa
     2281 gatcgaggac gtcacccttg agcaatggca ctcgctgctg gcggtcatgc tggacgggcc
     2341 attcctgatg tcgaaagcct gcattgcgag catgaggcag cggggctcgg gccgcatcgt
     2401 caatatcggc ggcatgaccg gacataccgg cgcgccggaa cgcgccgcga cggtaaccgg
     2461 caaggctggc ctagccggcc tgacgcgcgc gctggcgcat gatctcgccg gcgacggagt
     2521 gacggtcaat tgcgtggtgc cgggcatgat cgatacggtg cgcggcggct ccaacgcact
     2581 cgcgccggcc catcatgcca cgcgaaacct cttgctgggg cgccggggac gtcccgaaga
     2641 agtagccgat ctcgttgcat atctttgcgg cccgctggcc ggcttcgtga cgggccaatc
     2701 cattcatgtt aacggcggta cattcatggc ttgaacgcaa tggtttcgat tcgccgggga
     2761 taatgaacca gacgcggcgg gctggagggg aactggcttg gactacctga cgcgtgcccg
     2821 atatgccggg tcgttccgtg cgagaccgaa gaacggcttg gctggccgag gccggaagtg
     2881 gaatatcgca tgacccccgc cagcgcctcc atggtccatc agccggagct caagccgctc
     2941 taccttcaga tacaggaaat gctcatcgag cgcgtggtca acggcgaatg ggggccgggt
     3001 gagatgctgc cgtcggaaat gcgcctggcg gtggaatata acgtccacca gggcacggtg
     3061 cgcaaggcgc tggacgcgat ggcgatgcgc aatctcatcg tgcgccatca aggcaagggc
     3121 accttcgtcg ccagcgtgtc gatgcgccac agccccttcc gttacttcaa gctcgtcttc
     3181 ctgaataatc ctgccgaacg ccctgtcagc catttcattt cgatcaccgt tgcccaggcg
     3241 tcgaaacagg aacaatcggc gctccagctg gccggtcccg aagacaaggt cgtgcgcgct
     3301 accaaggtgc gcgcctatgg cgccgtcccg gcgataatgg aggaaatagc ctacccggag
     3361 ggcatgtttc ccggattggc cgacctgttc cgcaagcttc atcccgacac gacctacgga
     3421 ctgctggagc agaaataccg cgtgctgatc accacggtaa aggaccagat cggctcggcc
     3481 gccgccacca gcctcgatgc ggagcacctg aaagttgcca tcggcgagcc gcttctgcgc
     3541 atcaaccgta ccgccttcgc cgttgacggc cgccccgccg agtggcgcgt cagccgctgc
     3601 acgaatgcct gcgaatacgt tctcgatcag atgtgatcgg ttggcgatgc gcaagcatcg
     3661 gtgaccggtg agcaggggcc ggtaaccggt cagcgagaca agccgctgtg accggtcatc
     3721 agggcgcagc agcggtgagc gcggggcgag cagcggcgat tgtcggcaat tgactcgtca
     3781 gtacctctta tataagatat atgtatggca tggatcggga ggtcggttcc agccgatggg
     3841 aggatattat gtggaataga cgcgctgttt cggctggttt gctgttgggg cctcttcttt
     3901 gggccgtccc gactttcgcc caggaaaaca aatacgacta tccggtcacc accgttacct
     3961 tgaccaccca ctccaagccc ggcgccggtt cggacttgtt cctgcgcaag ctgagtgcgg
     4021 ccctgtcgaa ggaaatgggc atcaacttcg tcgtcgatta ctggacgggc ggcagcggcg
     4081 ccaaggcgat ggcggaactg gcgaaggcca agcccgacgg ctcggttttc tatgcgacga
     4141 cgccgacgca catcaacacg tccctgctca gcgagccgcc ggttacctat aaggacatcg
     4201 actacatcgc caacgtgttc ttcgacccgg agatcatcta cacgctctct tccagcccct
     4261 tcaaaacgat gaaggacgcc gtcgatgcag cgaaggcctc gccgggaagt cagatctggg
     4321 gcggatcgac ccccggatcg ctggagcgtc agattctcga acgcatcaac cagcacacgg
     4381 gcgccggcgc ggtagtagtg ccgcacgacg atggcgccgg tcttttgatc gacgtcctca
     4441 acggcacggt ctcgctgggc gtcggggaat tgcaggaagt gcaaagctac cttcaggccg
     4501 gcaagatgaa agtcctggcg gtttactatc cgcagcgtct ggaaatgttg ccggatgttc
     4561 cgaccatcca ggaactggga ctagacaccg aaaccgtccg caagtttcgc ggtctggccg
     4621 ggccgaaggg cattccgccg gagaccgtga aaaagatcga cacggccatc gaggcaattc
     4681 tcaaggacaa ggacttcatc aagatttata gcgaggataa ccttgtgcct ggctatatga
     4741 accaggaaga ctaccgcgct ttcatggtaa agttcgtgga agctgaaaaa ggcttcatca
     4801 aggacttcgg aatcaagaac gactgaactt gagtatgctg gtgggcctgc cggaatacgg
     4861 gggtcgccgt aagcaatcgg catggtaaaa atggcgcggc agcgtccgtt gaaaacaagc
     4921 ggccgggatg gatttcccgg ccgcttgttt tttgtccgga cgcttgcttt gtcccgtcga
     4981 ggcccggatt gctcctttcc ggccggatat tcccgtttga ccgccgggaa ttcatttgag
     5041 atcgaccgag cagcggcgca atcaatcgtg ccaggcgatt agaatgatct tctaaatcaa
     5101 ttggagaaaa gagaaggatt tcgctaggaa gcgtggaaat catgtaattc cgagaattcg
     5161 caaatgacca ttactgcaac tgccccccaa tcacgcgccg ataacgccct tgcagcctat
     5221 gtgccgggtc ttgtcctggt tgcggtcatc acggctgccg cttacggcgt caggcacctt
     5281 ccgttcatgt cgccgctcag cccgatgatc cttgcgatct tcgtcggcat gctgtttgcc
     5341 aatgttgtcg gcagcaatat ttcggcggcg gaaggaatct ccatttccgg caaacggctt
     5401 ttgcggcttg ccgttgcttt gctcggcctt cagatatcgt tcgcgcagtt ggcggagatc
     5461 ggtgtccgcg gcgtcggcct ggccgcaatc ggcctgtttt ccacatttct cttcacgatc
     5521 tggatgggca aactgttggg cgtagaccgc aatgtcgcgc gcttgcttgc ggcgggtacg
     5581 gccgtttgcg gcgcatccgc gattgccgcg gccaatgcgg tcaccggcgc cgacgatgaa
     5641 gacacctcct acgccgtcgc ctgcatcacc ctgttcggca cgatagcgat gttcctgttc
     5701 ccgttcctga acttcgtcgc cgggctgtcg agccacgcct atgggctatg ggtcggcctg
     5761 tcggtccatg aggttgcgca ggtggtcgga gcgggcttcc agggcggcaa ggaagcgggc
     5821 gaaatcgccg tcatcaccaa gctttcccgc atcatgatgc tggctccgct gatcatcggc
     5881 atgtcctttg ttctctcgcg gtcttcgagc agggcttccg acaagaaagg gcaggtgccg
     5941 ctggtgccgt tcttcgtggt gggcttcctg gtgcttgccg cactcaacag cttcggcctg
     6001 gtgccggagg ccatccgttc gccgatcgtc accgcaacgc cggtcttgct gaccgccgcc
     6061 atggcagcac ttggcctggg caccagcgtg tccaagctca agcagcacgg cctgcgtccg
     6121 ttcctgcttg ccggcatggc caccgtgttc atcgccggcg tcggcttcct gatggtgacg
     6181 ctggtggcgt gatagtgctg tgccgtgcgg cctcggtggg ctaaacaagc caaccggccc
     6241 cccaatttgg ggcaatgcgg ataagaggtc tttgtgacgt tcgatcaact caggatattt
     6301 ctggccgttg ccgagcgcga gcatgtgacg cgggcggccg atttcctcaa cctcacccaa
     6361 tcgacggtca gcggcgcgat caccgcgctg gaagcgcggc acaacgtaaa gctgtttcat
     6421 cgggttggcc ggcgcatcga attgacggac gaaggccgga tattcgcaga cgaagcgcgg
     6481 gcaattctgg ccgcggtccg ctctgctgaa accaccttgg cggatttggg cggcgccccg
     6541 cgcggcgcgc tctccatctt tgccagccag acgattacca gctattggct accgcaatat
     6601 ctggtcgggt tccacaagct ttatccgcag atcgatctgc atctggaggc cggaaacacc
     6661 gcccaatgcg ttcgcgccgt aaatgccggc tcggccgaca tcggtttcat cgaaggaacg
     6721 atcaacgagc ctggcgtttg caccaggctg gtggcgcgcg atcgtctcgt catcgtggtg
     6781 ggcgcagacc atccatgggc gaaaaagccg ccgcaatcgc ctctggagct tgtcgagcag
     6841 caatgggtcc tgcgcgagaa aggttcgggc acgcgctcaa gcttcgaaga agccctgagc
     6901 gcgcacgcca tccagccgtc gcagcttcgc gtcgcgatcg aacttccctc caatgagggc
     6961 atttgcaccg ccgtggcaac cggtcagtat ccgaccgccg tttccgagac ggtcgccgag
     7021 gcgggcgtga attccggaag gctggtgacg gtgccgttcg aactgccctc gcgggcattc
     7081 agcctcatcc ttcacaggga gcgccaccag agccgcgcgt gcaaggcgtt ttgtgagatg
     7141 ttgccggcac gcgaaagcct ttaacgcgcg atgcagcgct tccgtcaggt aataagtagt
     7201 atatggagaa attccgctgg tggagctgag gaggatcgaa ctcctgacct cgtcattgcg
     7261 aacgacgcgc tctcccagct gagctacagc cccatcagcg gatggggcat ttatgaggcg
     7321 aggccggtga ctgtcaagca tatgcgcatg ccggacgcca gatttcccat ggtcgtgcgg
     7381 tgccgattgc gcgggcggct tcgccggctt gcggcacgcc tgtccggcac gggagaggtg
     7441 gggtcttgcc ggttccctcg tcagtggcta catgtcggcc agttgatgga ggtttgaatg
     7501 aacgcgctaa tcgatacgct gatctacgcg ctcagcctct actggtggat catcatcgcg
     7561 tcggccattt tttcgtggct gttcgccttc aacgtggtca attcccgcaa ccagttcgtc
     7621 aataatatcg gcacggtgct ttatcggctg actgagccgg tttatcggcc tatccgccgc
     7681 tatctgccgg atctaggcgg catcgacata tcgcccatca tcgtgttgct gatcatcttt
     7741 ttcctgcaac ggtttctcga cacgacagtc cgggccgcct tttactatta agtttcgcca
     7801 gccgggcttc gccagtgcta ccggccctgc gcgaggtcga ggcagcgttg ccacagatcg
     7861 gcatcgacgt cgatcaagat tgaatcccga tcctgtttgc ccgctgtcgc tccgctgccc
     7921 ggcaatcgca ctccggcggc ctcgaaactt tccgcaaggg cgccaaggct ttccgcaaag
     7981 cgggcgctga agcgggcggg atcgatcgcc agcgtgaatt ggccaacgcc gggcggcccg
     8041 ccttgatcgt cgccgaacat gctggcctgc gaactcagct tcgccccggt tagcgccgtc
     8101 gccaatattt cgatcatcag cgccagattg gctccctttt gcccaccact cggcagcagc
     8161 gatccgcgca ggccttcgcc tgcgtcggtg gtcggcgcgc cggacccgtc ttgcgcccag
     8221 ccggtgggta tcggctggcc ccgg
//
LOCUS       DASYCS010000047         4674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4936671,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000047 DASYCS010000000
VERSION     DASYCS010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4674
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4936671"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..90
                     /locus_tag="VGM46_01875"
     CDS             <1..90
                     /locus_tag="VGM46_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HEY4191368.1"
                     /translation="AEVKDVWTFARDTRSRDPNWKLVATEEED"
     gene            115..1221
                     /locus_tag="VGM46_01880"
     CDS             115..1221
                     /locus_tag="VGM46_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HEY4191369.1"
                     /translation="MSLSSLFRPVPFTALPGWGQRDHLPAFEAFRRSAFHVLNKPYRT
                     GALGVAFDGFAEAYAAARTVSPANRAEAQAFFEHFFVPALIVPEDGQAGLVTGLYEPQ
                     AKASPVRTETFSVPLLSRPADLIDVDDADHPAGMDPYLAFARKTPDGLVEYFERGEIE
                     RGALCGRGLAIAWLADQVEAFFIHVQGAARLAMTDGRLVRVTYAAKSGQRFSGPGRIL
                     ADLGEIPLEKVTMQSIRAWFKANPDRIDEILWQNRSYIFFREAAVDDPALGPVAAAKV
                     PLTPGHSVAVDRLLHTFGTPFYIDAPGLTAFGGESFRRLMIAQDTGSAITGPARGDLF
                     AGSGDAAGEIAGVVRNAADFYALLPLGLVSGPNR"
     gene            1218..1748
                     /locus_tag="VGM46_01885"
     CDS             1218..1748
                     /locus_tag="VGM46_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEY4191370.1"
                     /translation="MKRQDGGLKEEDRVLWSLVARTAMPLKTTRAVVVPEPSPEARAM
                     PLEPARVAIAPGGAPKVKSLRVSHILDEPTLDKLSKGRLPIEGRVDLHGLTQDEAYSL
                     LFSFLQRAHAGGVRYVLVITGKGSSSGGDGVLRRSVPAWLSTPAFRLLVSSHDHAARQ
                     HGGSGALYVRLRRTRP"
     gene            1774..2139
                     /locus_tag="VGM46_01890"
     CDS             1774..2139
                     /locus_tag="VGM46_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY4191371.1"
                     /translation="MTPLGERIRALRRERSVSQKEMAAAVGVSAAYLSALEHGRRGAP
                     TWALTQKIIGYFNIIWDDAEELELLAQASHPRVTLDTSSLSPAATALANLLAAEIGRL
                     GEADLLALAASIREMASRR"
     gene            2396..2605
                     /locus_tag="VGM46_01895"
     CDS             2396..2605
                     /locus_tag="VGM46_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEY4191372.1"
                     /translation="MSTGTVKWFNATKGFGFIQPDDGSADVFVHISAVERAGMRDLAE
                     GQKLDYEMVRDNRSGKMSADQLKAA"
     gene            2663..2803
                     /locus_tag="VGM46_01900"
     CDS             2663..2803
                     /locus_tag="VGM46_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191373.1"
                     /translation="MEGQANAWPFSFAILFAAQSLEAIPGNIGTDPDFWRGDEFCFIR
                     IM"
     gene            2820..3053
                     /locus_tag="VGM46_01905"
     CDS             2820..3053
                     /locus_tag="VGM46_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191374.1"
                     /translation="MSDTSKRSRDQAESIFSKTQTQAMSLNRIISEQDAASNARVAKT
                     ARLRELRLEKEAAALAVEAASPKAASSKNAGKR"
     gene            3053..3454
                     /locus_tag="VGM46_01910"
     CDS             3053..3454
                     /locus_tag="VGM46_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191375.1"
                     /translation="MAKRGAEDAIAVDSTVADIVEKLDKPVEYVRRVLEKLEYCRRIH
                     GDAQVRVGVLSRPECPNYLIEYIHTDKKTRETSIRPQAAFSGSTHRELAPRHIDDPRN
                     WSPEEMNITAVAALIGRLRNPRAVSRFSDED"
     gene            3458..3631
                     /locus_tag="VGM46_01915"
     CDS             3458..3631
                     /locus_tag="VGM46_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191376.1"
                     /translation="MAIRFSSKEQPAVPASKGGKPAPEAETKKPAAEPDASDLFDPDD
                     GQPSAKAKGRKKK"
     gene            3631..3960
                     /locus_tag="VGM46_01920"
     CDS             3631..3960
                     /locus_tag="VGM46_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019857423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEY4191377.1"
                     /translation="MPAAAFSPGPESLAPGFDCKSCGACCSYSADWPRFSTEDDAQLD
                     RIPAKLVAANETGMRCDGVRCSALSGVVGKATACSIYELRPDVCRACMPGDGECRMAR
                     QSFGLPV"
     gene            complement(4061..>4674)
                     /locus_tag="VGM46_01925"
     CDS             complement(4061..>4674)
                     /locus_tag="VGM46_01925"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY4191378.1"
                     /translation="GNASAGLSKARVPQRKRGRDRVAALMAGAAALFMEKGFEAATMT
                     EIAARSGAAIGTLYLFFPTKEALAQAILAEHAEDLSTRLDALRQRTEGLSADTIAGAL
                     FSLLGDFLAEHPVYSALLDLTGDESWRRAMRARRRQQIAALFSQAKPCLPEGQAERLA
                     VIVPQLMRIPLSLSGEARLREGVLEELRVMLCRHLQSDVGEHI"
BASE COUNT          841 a         1514 c         1462 g          857 t
ORIGIN      
        1 gccgaggtca aggatgtctg gaccttcgcc cgcgacaccc gctcgcgcga tccgaactgg
       61 aaactggtcg ccaccgaaga agaagattga ccgcaaggcg gcggcgaggc tgtcgtgtcg
      121 ctctcttccc tttttcgccc ggtgcctttc acggctttgc ctggctgggg ccagcgcgat
      181 cacctgcccg ccttcgaggc tttccgccgc tcggccttcc atgtccttaa caagccttac
      241 cgcaccggtg cgcttggcgt cgcctttgac ggctttgccg aagcctatgc cgccgcgcga
      301 accgtttcgc cggcgaatcg agccgaggcg caggccttct tcgagcattt cttcgtcccg
      361 gccctgattg ttcccgaaga tgggcaggca ggcctcgtca ccggcctcta cgagccgcag
      421 gctaaggcgt ctcccgttcg caccgagact tttagcgtgc cgctgctgtc gcggccggcc
      481 gatctcatcg atgtggacga cgccgaccac cccgccggca tggaccccta cctcgccttt
      541 gcccgcaaga cgccggacgg tctggtggaa tatttcgagc gcggcgagat cgagcgcggc
      601 gcgctttgcg gccgggggct ggcgatcgcc tggctggccg accaggtgga ggccttcttc
      661 atccatgtgc agggcgccgc gcgccttgcc atgaccgatg gccgactggt ccgcgtcacc
      721 tatgcggcca agtccggcca gcgcttcagc ggtcccggcc gcattctggc cgatctgggc
      781 gagattccgc tcgaaaaggt gacgatgcag tccattcggg cctggttcaa ggccaatccg
      841 gaccgcatcg atgaaatcct ttggcaaaac cggtcctaca ttttcttccg cgaggcggcg
      901 gtggacgatc cggcgctcgg cccggtcgcg gccgccaagg tgccgctgac gcccggccat
      961 tcggttgccg tcgaccggct gctgcacacg ttcggcacgc ccttctatat cgacgcgccc
     1021 ggcctgacgg cctttggcgg cgaaagcttc cgccggctga tgattgcgca ggacaccggc
     1081 tcggcgataa ccggcccggc gcgcggcgat ttgtttgccg gttcgggcga tgctgccggc
     1141 gagattgccg gcgtcgtgcg caatgcggcc gatttctatg cgcttctccc gctcggcctt
     1201 gtttcggggc cgaatcgatg aagcggcaag atggcgggct gaaggaagaa gaccgggtgc
     1261 tgtggagcct ggttgcccgc acggcgatgc ccttgaaaac gaccagggcg gtcgtcgttc
     1321 cagagccttc ccccgaagcg cgggcaatgc cgctcgaacc ggccagggtc gccatcgcgc
     1381 ccggcggcgc gcccaaggta aaaagcctgc gcgtctcgca cattctcgat gaaccgacgc
     1441 tggataaatt gtccaagggg cgactgccga tcgaaggccg ggtcgacctg catggcctga
     1501 cgcaggacga ggcctattcg ctgctgtttt cgttcctgca acgcgcccat gccggcggcg
     1561 tgcgctatgt gctggtcatt accggcaagg gctcgtcttc cggcggcgac ggcgttttgc
     1621 gccggtcggt gccggcttgg ctgtcgacgc ctgcctttcg gctgctggtt tccagccacg
     1681 accatgcggc gcgccagcat ggcggttcgg gcgcgcttta cgtgcgtttg cgccggacgc
     1741 ggccatgagg agtggcaaga ggagggcggc ggcatgacgc cgctgggcga acgcatccgc
     1801 gccttgcggc gcgagcgcag cgtcagccag aaggaaatgg cggctgcggt cggcgtcagc
     1861 gccgcctatc tttcggcgct ggagcatggc cgccgcggtg cgccgacctg ggcgcttacc
     1921 cagaagatca tcggctactt caacatcata tgggacgacg cggaggagtt ggagcttctg
     1981 gcgcaggctt cgcatccgcg cgtcacgctc gacacgtcca gcctgtcgcc ggcggcaacc
     2041 gcgctcgcca accttctcgc cgccgagatc ggcaggcttg gcgaggccga tcttttggcg
     2101 ctggccgcct ccatccggga aatggcgtcg cgacgatgat gggaccgccg ctctgtcgtc
     2161 atgcatcgga ggctgccggg gaagctgtct ggcaggcccg taaaagccgc cgcctgacgg
     2221 aaagttgact cagcgcccaa aaatccctac atacgtcatg tcgatatttg cttgaccgac
     2281 ttttgttatg cgtgcgtgac gcgcgctctt gacgaacttc ccatcaaaaa tcgggacgaa
     2341 agccgcagcg cagtcgcgcg gcggtgaacg actattgcca tggaaagggt tcgctatgag
     2401 caccggcaca gtaaagtggt ttaacgccac caaaggcttc ggcttcattc agccagacga
     2461 cggttcggcc gacgttttcg tacacatctc cgctgtcgag cgcgccggaa tgcgcgattt
     2521 ggccgaaggc cagaagcttg actacgagat ggtccgcgac aacaggtccg gcaagatgtc
     2581 cgccgaccag ttgaaggcgg cctgacagga attcgatgca ccggtgacca cggatgtact
     2641 ggcatcggtg taatgaattt ccatggaagg tcaggcgaat gcctggcctt tttcatttgc
     2701 gatcctgttc gccgcgcaat ctttggaagc aattccggga aatataggaa ccgatccgga
     2761 tttctggcgg ggcgatgaat tttgctttat caggatcatg tgaggtcagg agcatgacag
     2821 tgagcgatac gtcaaaacga tcccgcgacc aggccgaatc cattttcagc aagacgcaga
     2881 cccaggccat gtccctgaac cggatcattt ccgaacagga cgccgccagc aatgcacgcg
     2941 ttgccaagac cgcgaggctg cgcgaattgc ggctggaaaa ggaagccgcc gctcttgccg
     3001 tcgaggcggc atcgcccaag gcggcatcgt ccaagaatgc ggggaaacgc tgatggccaa
     3061 acgcggggct gaagatgcca tcgctgtcga ttcgactgtc gccgatatcg tcgaaaagct
     3121 cgacaaaccg gtcgaatatg tcaggcgcgt cctggaaaaa ctggaatatt gccggcgcat
     3181 tcatggcgac gcacaagttc gtgtcggcgt tctcagccgc ccggaatgcc cgaactacct
     3241 gatcgaatat atacatacgg acaagaaaac ccgcgaaacc tccatacgcc cgcaggcggc
     3301 tttcagcggc agcacccacc gcgaactggc gccccgtcac atcgacgatc cccgaaactg
     3361 gtcgccggaa gaaatgaaca ttaccgctgt cgcggcgctt atcggccggc tgcgcaatcc
     3421 gcgcgccgta tcccgctttt cggacgagga ttgaaccatg gcgatccgtt tctccagcaa
     3481 ggaacaaccg gccgtgcccg cttccaaggg cggcaagccg gccccggaag ccgagacgaa
     3541 aaaacccgct gccgagccgg acgcaagtga cctgttcgac cccgatgacg ggcagccctc
     3601 cgccaaggcc aagggccgga agaagaaata gtgccggcgg cggccttttc gcccggtcct
     3661 gaaagcctgg cccccggctt cgactgcaaa agctgtggcg cctgctgttc ctattccgcc
     3721 gattggccac gcttttccac cgaagacgac gcccagctcg accgtattcc ggccaagctg
     3781 gttgctgcca acgaaaccgg catgcgctgt gatggcgtgc gttgttcggc gctttccggc
     3841 gttgtcggca aggccaccgc ctgttcgatc tacgagcttc gcccggatgt ctgccgcgcc
     3901 tgcatgccgg gcgacggcga gtgccgcatg gcccggcaat cattcgggct gccggtctaa
     3961 agcatttcca gcaaaagcgt aaaacggttt tgcgtccgga aatgcggcca aaacaaagag
     4021 atagagcggt tttgtcgatt cggtaaaaat cggaaccgct ctagatgtgc tcgcccacat
     4081 ctgattgcag atggcggcac agcatcacgc gcaattcttc cagcacgccc tcgcgcagcc
     4141 ttgcttcgcc cgacaaggaa agcgggatgc gcatcagttg cggcacgatg acggccagcc
     4201 gctcggcctg tccctccggc aggcacggct tcgcctgcga gaacaaggct gcgatctgct
     4261 ggcgccgcct tgcgcgcatg gcgcgccgcc agctctcgtc gccggtcagg tcgagcagcg
     4321 ccgaatagac cgggtgctcc gccaggaagt cgcccagcag cgaaaacagc gcgccggcga
     4381 tcgtatcggc cgaaagtccc tccgtgcgct gccgcaatgc gtccagccgc gtcgacaaat
     4441 cctccgcatg ctcggccagt atggcctgcg ctagcgcctc cttggtcggg aagaacagat
     4501 agagcgtgcc gatggccgcg cccgagcgcg ccgcgatctc ggtcatcgtc gctgcctcga
     4561 agcccttttc catgaacagc gcagccgcgc ccgccatcag ggcggcgaca cggtcgcgcc
     4621 cgcgctttcg ctgcggcaca cgggcttttg ataaaccggc gcttgcgttt cctg
//
LOCUS       DASYCS010000048         3484 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3923088,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000048 DASYCS010000000
VERSION     DASYCS010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3923088"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1040)
                     /locus_tag="VGM46_01930"
     CDS             complement(<1..1040)
                     /locus_tag="VGM46_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY4191379.1"
                     /translation="MGIEGIGARVARKEDKRFITGAGRYVDDMVVPGMKHAAFVRSPY
                     AHAQIKKIDVKAAQAMPGVIGVLTGKEIKADGIGNLICGWMIHSKDGTPMKMGAWSPL
                     AFDKVRYVGDAIAIVVADTKGQARDAAEAVEITFKELKSVTDASKAMEKGAPQIHPEA
                     ENNLIFDWDIGNADETAAAFKKAAHIVEMDVINNRLVPNAMEPRAALGHYDKAEDHYT
                     CWTTSQNPHVARLVMSAFYNVAPENKLRVIAPDVGGGFGSKIYIYPEEIVCLWASKKT
                     GVPVKWVADRTESFLCDAHGRDHQSHCAMAFDKDHNILGFKVETKANLGAYMSLFSSA
                     TPTYLYATLLSG"
     gene            complement(1183..1698)
                     /locus_tag="VGM46_01935"
     CDS             complement(1183..1698)
                     /locus_tag="VGM46_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011579406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY4191380.1"
                     /translation="MSNVSLTVNGRNISGTAEDRTLLVHFLRENLGLTGTHVGCDTSQ
                     CGACVVHVDGKAVKSCTMLAVQASGSTVLTIEGLADGADLNPMQAAFKEHHGLQCGFC
                     TPGMIMTATDMVRRHPEGLDEATVRAELDGNICRCTGYHNIVKAILAASKTMAKTAKA
                     KTPKVKTKQAA"
     gene            1958..3130
                     /locus_tag="VGM46_01940"
     CDS             1958..3130
                     /locus_tag="VGM46_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HEY4191381.1"
                     /translation="MAFSRTTYACGLCALTTDDADTDAFACAVAAEAKAIDAGFALLF
                     FSQSLVEADALSRAIRLHAPALRHAGCSTAGEITPEGLEEGHMLAMLFPSGLFSVVST
                     MVDDLSSSGMDRITGEVEALRRSLLIKMDGREAGENIFALCFIDGLSHAEEAATSAIH
                     WGLADIPLIGGSAGDDLKFKTTRLISDGRVTSDSAIVVLVSTEIPFHVFKTDNFMPTD
                     EKLVVTSSDPDRRIVREFNAANAAEEYAESVGIDTPSLTPLSFASHPVVVKVGGEYYC
                     RSIQKMHPDGSLSFFCAIDDGVVLSIAKPLGMVESTRAALEDVEEKLGGIDMILGFDC
                     VLRRLDARNRQVFRGISELYRKHRVIGFGTYGEQYRSMHLNQTFTGIAFGQRHAAE"
     gene            3135..>3484
                     /locus_tag="VGM46_01945"
     CDS             3135..>3484
                     /locus_tag="VGM46_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006335353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEY4191382.1"
                     /translation="MPLTSITDVERLKKINAALVGRVERSMDQQGNAFSLFQTAIALE
                     NRIRTRTEELHSTLLRLEQSNIDLSAAKENAELANLSKTRFLAAASHDVLQPLNAAHL
                     SVSALAEIQTSGEGR"
BASE COUNT          665 a         1080 c          995 g          744 t
ORIGIN      
        1 tggcccgaca acagcgtggc gtagagatag gtcggtgtcg ccgatgaaaa cagcgacatg
       61 taggcgccga gattggcttt tgtttcaacc ttgaagccaa ggatgttatg atccttgtcg
      121 aaggccatgg cgcaatggct ctggtggtcg cggccatgcg catcgcaaag aaagctttcg
      181 gtgcggtccg ccacccattt caccggcacg ccggtcttct tcgaagccca caggcagacg
      241 atttcctccg gatagatata aatcttggag ccgaagccgc caccgacatc cggcgcgatg
      301 acgcgcagct tgttttccgg cgcaacgttg tagaacgcgc tcatcaccag ccgcgcgaca
      361 tgcgggtttt gcgaggtcgt ccagcaggtg tagtggtcct cggccttatc gtaatggccg
      421 agcgctgctc gcggctccat ggcattggga accagccggt tgttgatgac atccatttca
      481 acgatgtgcg cggccttctt gaaggcagcc gccgtctcgt cggcattgcc gatatcccag
      541 tcgaagatca ggttgttttc cgcctcggga tggatttgcg gcgcgccctt ctccatcgcc
      601 ttggaagcat cggtaaccga cttcagttcc ttaaaggtga tttccacggc ctccgcagcg
      661 tcacgcgcct gccccttggt gtcggccact acgatggcga ttgcatcgcc gacatagcga
      721 accttgtcga aggcaagcgg cgaccacgcg cccatcttca tcggggtgcc gtccttggaa
      781 tggatcatcc agccgcagat gagattgccg atgccgtcag ccttgatttc cttgccggtc
      841 agaacgccga tcacgcccgg cattgcctgc gcggccttca cgtcgatctt cttgatttgc
      901 gcgtgtgcgt atgggctgcg gacgaacgcc gcatgcttca tgcccggcac gaccatgtcg
      961 tcgacatagc gccccgcacc ggtgatgaac cgtttgtctt ccttgcgcgc aacgcgtgcg
     1021 ccaatgcctt caatgcccat cagagttctc ctccagaact gttagaagcc gatttcgcgt
     1081 agtcgtcgga agcgatggcc ccggtgttag cgttggttgc cggcgttggc gggcaacaaa
     1141 aaggccctgg ccatcacgca tagcggccat tcgccacctg tctcacgcag cctgcttcgt
     1201 ctttactttc ggcgtcttgg ctttcgctgt cttggccatc gtcttcgatg ccgccaaaat
     1261 ggctttcacg atgttgtggt agccggtgca gcggcagata ttgccgtcca gctcagcgcg
     1321 caccgttgcc tcgtccagcc cttcgggatg gcggcggacc atgtcggtcg cggtcatgat
     1381 catgcccggc gtgcagaagc cgcactgcaa tccatgatgt tccttgaaag cggcctgcat
     1441 cggattgagg tcggcgccat cggcgagacc ttcaatggtc aacaccgtcg atccggacgc
     1501 ctgtacggcc agcatcgtgc atgatttgac cgccttgcca tcgacatgga cgacgcaggc
     1561 gccgcattgc gaggtatcgc agccgacatg cgtgccggtc agcccgagat tttcccgcag
     1621 gaaatgaaca agaagcgttc ggtcctcggc agtgccgctg atgtttcttc cattcaccgt
     1681 caatgacaca ttcgacatga aacctccctg ggtggtcaac ctcggtattc atcctccacg
     1741 ccggccatcc ctgccggcgc agcgttattg atatctcgtg caacgcttga gaaacagccc
     1801 tctggaatcc ctatgccatg agaatgcagc ccgtccgaaa aggcgtatat tgtactttcg
     1861 gtcacgcgct gagcgccatt gccaaacccg ttacaccaga cgacaatagc gctcgacgcc
     1921 ggttacaggc ggagcgaggc tatcagggag gaaccggttg gccttctcgc gaacgactta
     1981 cgcttgcggt ctttgcgcgc tgaccacgga tgacgcggac acggatgcgt tcgcctgtgc
     2041 cgtggccgcc gaggccaagg ctatcgatgc aggctttgcc ctgttgtttt tctcccaaag
     2101 ccttgtcgag gccgacgcgc tttcgcgggc gatccggctg cacgcgccgg cgctgcgcca
     2161 tgccggttgc tccactgccg gcgaaatcac gccggaaggg ctggaagaag gccatatgct
     2221 ggccatgctt ttcccctccg gccttttttc ggtcgtaagc acgatggtgg acgatctttc
     2281 atcgtcaggc atggacagga tcaccggcga ggtcgaagcg ctgcgccgct cgttgctcat
     2341 aaaaatggat ggaagagagg ccggcgagaa cattttcgcg ctctgcttca ttgacggtct
     2401 ttcccacgcg gaggaagccg cgacttccgc catccattgg ggattggctg atattccgct
     2461 gatcggcggg tcggccggcg acgacctgaa gttcaagacg accaggctga tttccgacgg
     2521 tcgcgtcaca tccgacagcg ccatcgtcgt ccttgtctct accgaaatcc cgttccacgt
     2581 cttcaagacc gacaatttca tgccgaccga cgaaaagctc gtcgtaacgt catccgaccc
     2641 ggatcgacgc atcgtgcgcg aattcaatgc cgccaacgcc gcggaggaat atgcggaatc
     2701 ggtgggcatc gacactccgt cgctgacccc gctcagcttt gcctcgcatc cggtggtggt
     2761 gaaggtaggc ggcgaatatt attgccgctc catccagaag atgcacccgg acggctcgct
     2821 atccttcttc tgcgctatcg acgacggcgt ggtgctgtcc atcgccaagc ccctgggcat
     2881 ggtggaatcg acgcgcgccg cccttgagga cgtggaggag aagctgggcg gcatcgacat
     2941 gatcctggga tttgattgtg tcctgcgccg cctcgatgcc cgcaatcgcc aggtttttcg
     3001 cggcatatcg gagctttacc gcaaacatcg cgtcatcggc ttcggcacct atggcgaaca
     3061 atatcggtcg atgcacctga accagacttt taccggcatc gcgtttggcc aacgccacgc
     3121 ggcggaatga tgcaatgcct ttaaccagca taaccgacgt cgaaaggctg aagaaaatca
     3181 atgcggccct ggtcgggcgt gtcgagcgct ccatggacca gcagggaaac gccttctcgc
     3241 ttttccagac ggcgatcgca cttgaaaacc gtatccgcac ccgcaccgag gaacttcatt
     3301 ccacgctgct gcggcttgaa cagtcgaaca tcgacctcag cgccgccaag gaaaatgccg
     3361 aactcgccaa tctttccaag acgagattcc tcgccgccgc cagccacgac gtgctgcaac
     3421 cattgaacgc cgcgcatctt tcggtgtcgg cgctggctga aatacaaacc agcggcgaag
     3481 gtcg
//
LOCUS       DASYCS010000049         2831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_880497,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000049 DASYCS010000000
VERSION     DASYCS010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_880497"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="VGM46_01950"
     CDS             complement(<1..861)
                     /locus_tag="VGM46_01950"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEY4191383.1"
                     /translation="MELDPLFLSRLQFAWLIAWHILMPALTVGLASFIALLEGLHFFT
                     GKDIYFRISTFWIKVFSVAFGIGVVSGIIMPFQFGTNWSRYSDVTANVLAPLFAYEGL
                     TAFFLEAAFLGVLLFGRKLVPAWAHFFAALMVAFGTLLSSFWILAANSWMQTPAGYET
                     IDGRFFPTDWMQIVFNPSFPYRFAHTVVGFYITTGFVVIAVGAYLLRKRAASPEARAM
                     MSITLWLLTLLVPLQIFLGDQHGLNTLEHQPAKLAAMEALWETTTEMPLVLFTIPSDT
                     AEANRFTVEIP"
     gene            1101..1931
                     /locus_tag="VGM46_01955"
     CDS             1101..1931
                     /locus_tag="VGM46_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003531108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HEY4191384.1"
                     /translation="MRELSEFPADAAARIRGVFCDIDDTLTTEGRLTADAYAALERLR
                     DAGLAVAPITGRPAGWCDMIARFWPVTGVIGENGAFYFAYDEATRKMIRVFAATDTER
                     AANRRKLEGVKRRILQEVPGAGISADQAYREADLAIDFCEDVPALPIGEVERIKRIFE
                     EEGAVAKISSIHVNGWFGQYDKLSMTRRFAGDRLGVDLDAAKDGYVFVGDSPNDAPMF
                     GFFPNSCGVANVRDFIGRIEAEPAYVASRRGGAGFVEIAEMILAHRRPQAARSGRVRE
                     "
     gene            1971..2156
                     /locus_tag="VGM46_01960"
     CDS             1971..2156
                     /locus_tag="VGM46_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008840190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191385.1"
                     /translation="MKSDEREKLDSLSKRLREEQKTLLLAAAQSANLPSQSTLQRVAQ
                     LELNISAIENTLADEAS"
     gene            2387..>2831
                     /locus_tag="VGM46_01965"
     CDS             2387..>2831
                     /locus_tag="VGM46_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO family protein"
                     /protein_id="HEY4191386.1"
                     /translation="MAGLAKTAGLAETAGPSGERTLEPPSFDNSYARLPDRFFAVQQP
                     NPVSAPQLVRVNGPLAAELGLDPQWLESPAGIDMLAGNRFPEGAEPIALAYAGHQFGN
                     FTPQLGDGRALLVGEVIDSHGVRRDIQLKGSGPTRFSRNGDGRAAL"
BASE COUNT          566 a          815 c          947 g          503 t
ORIGIN      
        1 cggtatctcg acggtgaagc ggttggcttc ggcggtgtcg ctgggaatgg tgaaaagcac
       61 cagcggcatt tcggtggtcg tttcccaaag cgcctccatc gccgccagct tggccggctg
      121 atgttccagc gtgttgagac catgctggtc gccgaggaag atctgaagcg gcaccagcag
      181 ggtcaaaagc catagcgtga tcgacatcat ggcgcgggct tcgggcgaag cggcccgttt
      241 gcgcagcaga taggcgccga ccgcgatcac cacgaaaccg gtggtgatgt agaagccgac
      301 gacggtgtgc gcgaagcgat aggggaagga cgggttgaag acgatctgca tccagtcggt
      361 cgggaaaaag cgtccgtcga tggtttcgta gccggccggc gtctgcatcc agctgttggc
      421 ggcaaggatc cagaacgagg aaaggagcgt tccgaacgcc accatcaagg cggcgaagaa
      481 atgcgcccat gccggcacca gcttgcgtcc gaacagcaag acgccaagga aggcggcttc
      541 caggaaaaaa gccgtcagcc cctcataggc gaacagcggc gccagcacat tggcggtcac
      601 gtccgaatag cggctccagt tggtgccgaa ctggaacggc atgatgatgc cggagacgac
      661 gccgatgccg aaagcgacgg agaagacctt gatccagaag gtcgatatcc ggaaatagat
      721 gtccttgccg gtgaagaagt gcagaccttc cagcagcgcg ataaacgagg ccagcccgac
      781 ggtcagcgca ggcatcaata tgtgccaggc gatcagccag gcgaattgaa gccgtgaaag
      841 gaaaagcgga tcaagttcca tttgcgcgca ccccgctcga ccggtccgtt cggcgcccgt
      901 ggggcgcaat atcgaccgtc gataccgttc acgagtttgc cagcctatct cgattgaagc
      961 aatgcgtcag cgttggggct gtctgccgcg cgtcacgttg ctgctgcttc actttaacgc
     1021 gatggttcga gatagtcgta ttttgtagaa tcggtccatt cgagcgcaag acgcccgatg
     1081 gcttgaaatc aaggacaggc atgcgtgaac taagcgagtt tccggccgat gcggcagcgc
     1141 gcattcgcgg cgtgttctgc gatatcgacg atacgctgac gaccgaagga cgccttacag
     1201 ccgacgccta tgcggcgctg gagcgtctgc gcgacgccgg ccttgccgtc gcgcctatca
     1261 ccgggcggcc cgccggctgg tgcgacatga ttgccaggtt ctggccggtg acaggcgtga
     1321 tcggcgaaaa cggcgcgttc tatttcgcct atgacgaggc gacacggaag atgatccggg
     1381 tgttcgccgc caccgatacg gagcgggcgg caaaccggcg caagcttgaa ggggtcaagc
     1441 ggcggatact tcaggaagtg ccgggcgccg ggatatccgc cgaccaggcc taccgggaag
     1501 ccgatctggc catcgatttt tgcgaggatg tgccggcgct gccgataggc gaggtcgagc
     1561 gcatcaagcg catctttgag gaagagggcg cggtcgccaa gatatcctcg atccatgtga
     1621 atggctggtt tggccagtac gacaaactgt cgatgacccg ccgctttgcc ggcgaccggc
     1681 tgggtgtcga tctcgacgcc gccaaagacg gttatgtgtt cgtcggcgac agcccgaacg
     1741 atgcaccgat gttcggcttt ttccccaatt catgcggggt cgccaatgtc cgcgatttta
     1801 tcgggcgcat cgaggccgag ccggcctatg tggcgagccg gcgcggcggc gccggtttcg
     1861 tcgagattgc cgagatgatc ttggcgcatc gccggccgca ggccgctcga agcggcaggg
     1921 tgcgggaata gtctcttgag ccttgcggcg cgcgaggtta tagccggcag atgaagtctg
     1981 atgagcgcga gaaactcgat agcctctcca agcgtttgcg cgaggagcag aaaacgcttt
     2041 tgctggccgc ggcgcaatcg gccaacctgc ccagtcaaag cacgctgcaa cgcgtggcgc
     2101 agctggagct gaacatttcc gccatcgaga atacgctggc cgacgaggct tcctgaccac
     2161 ggatcggagt tcctggttcg ggaatagggc tggctgattc aggatagggc cggcggcggc
     2221 gcgtcgggcg gttgcgcggg ctcatgcggg cgagcgggct tgtgacggca aggcgccgtg
     2281 cttatctctg gcccgaagca ggcgtgatct tttcattgtg cgccgggaaa aaagagaggc
     2341 gcgcatgccg gacgcaattc ttgaaaaaaa agctggtctt gccaaaatag ctggccttgc
     2401 aaaaacagcg ggccttgccg aaacagccgg gccatcaggc gaaaggacgc ttgagccgcc
     2461 gtccttcgac aacagctacg cccggcttcc cgaccgtttc tttgcagtcc agcaaccgaa
     2521 cccggtatcg gcgccccagc tcgttcgcgt gaacgggcct ttggcggcgg aactcggcct
     2581 cgatccgcaa tggctggaaa gtccggccgg catcgacatg ctggccggca accggtttcc
     2641 cgagggcgcc gagccgatcg cgcttgccta tgcggggcat cagttcggca atttcacgcc
     2701 gcagctgggc gacggccgcg cgctgctggt cggcgaggtg atcgatagtc atggcgtccg
     2761 gcgcgatatc cagctcaaag gttcgggacc gacgcggttt tcccgcaatg gcgacgggcg
     2821 cgccgcgctt g
//
LOCUS       DASYCS010000050         2585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_10515, whole
            genome shotgun sequence.
ACCESSION   DASYCS010000050 DASYCS010000000
VERSION     DASYCS010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10515"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="VGM46_01970"
     CDS             <1..694
                     /locus_tag="VGM46_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY4191387.1"
                     /translation="AGKSTISRLLFRFYDVQEGAVLIDGQDIRHVTQESLRAAIGMVP
                     QDTVLFNDTIAYNIRYGRIGASEEDVRRAATLAQIDSFIARLPEGYRSMVGERGLKLS
                     GGEKQRVAIARTILKAPPILILDEATSALDSHTEQEIQAALDLVSKGRTTIVIAHRLS
                     TVISADEIIVLREGQIAERGTHAALLREKGLYASMWDRQREANEAEERLRIAKEADEL
                     GVVVRRRTSEVS"
     gene            803..1501
                     /locus_tag="VGM46_01975"
     CDS             803..1501
                     /locus_tag="VGM46_01975"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010915147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEY4191388.1"
                     /translation="MQLADTIRKAFVPIHRQGYPFIAAFAVITLFLGYFSSILFWIGL
                     ILTAWCAYFFRDPERVTPLDDRLVVSPADGVISAVGPAAPPRELGLGAAEMIRISVFM
                     DVFSCHVNRAPVRGRITRIEHRPGKFLNAELDKASAENERNGLVIESPNGTVAAVQIA
                     GLVARRIVCWAKAGGTLAVGERFGLIRFGSRVDVFLPLTATPRVAIGQTAVAGETVLA
                     EFGATLAAPLVRVS"
     gene            1509..2333
                     /gene="pssA"
                     /locus_tag="VGM46_01980"
     CDS             1509..2333
                     /gene="pssA"
                     /locus_tag="VGM46_01980"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEY4191389.1"
                     /translation="MVAPFKKFEPHGSGGPRIREIPLRMMFPNLITVLAICAGLSGIR
                     FGFEGRFEPAVVMVLLAAFLDGVDGRLARLLKATSKFGAQMDSLADIVNFGVAPALVL
                     YAYLLDRAGSPGWIATLLFAIACGMRLARFNVLDEDTEQPAWQAEYFVGVPAPAGAVL
                     VMLPLYLSFLGLEPTKTTALVSSGFTVLVAFLLVSRLPVYSGKSLKIPGDRMLFVILA
                     VALYILLLVSYPWYTLTASVAVYLVFLPFSMRAYSRRALREGNASLRPDVDERSPE"
     gene            complement(2350..>2585)
                     /locus_tag="VGM46_01985"
     CDS             complement(2350..>2585)
                     /locus_tag="VGM46_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010915149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191390.1"
                     /translation="AAVAIPDKKRGERIVLVTTADAADPETLRRAGKQAGVGELMVPH
                     DIVKVGEIPVLGSGKTDYTSARKLALDRLVVRI"
BASE COUNT          380 a          876 c          815 g          514 t
ORIGIN      
        1 cgcgggcaag tccaccattt cgcggcttct gttcaggttc tacgacgtgc aggaaggcgc
       61 cgtgctgatc gacgggcagg atatccgcca tgtgacgcag gagagcctgc gcgccgccat
      121 cggcatggtg ccgcaggata cggtgctgtt caacgacacc atcgcctaca acatccgcta
      181 tggccgtatc ggcgccagcg aggaggacgt gcgccgggcg gcgacgctcg cccagatcga
      241 ttcgttcatc gcccgcctgc cggaaggcta tcgttcgatg gtcggcgagc gtggcctgaa
      301 gctttccggt ggcgaaaagc agcgcgtggc gattgcccgc accatcctca aggccccgcc
      361 catactcatc ctcgacgagg cgacatcggc gctggacagc cataccgagc aggaaatcca
      421 ggcggcgctc gacctcgtca gcaagggccg caccaccatc gtcatcgcgc accgcctctc
      481 gacggtgatt tccgccgacg agatcatcgt gctgcgggaa gggcagatcg ccgagcgcgg
      541 gacgcatgcc gccctgttgc gcgagaaagg gctttacgcc tccatgtggg accgccagcg
      601 cgaggcgaac gaggccgaag agcggctgcg catcgccaag gaagccgacg agcttggcgt
      661 cgtggtgcgc cggcgcacgt cggaggtttc ctgaacgatc gaccctgcct tggacaggcg
      721 atccggccca gggattgttg cgttgcggtg catgggggtt tccggtagac aggcgcgatc
      781 cgaaacggga taagccggcc ccatgcaact tgctgatacg atcagaaaag cgttcgttcc
      841 gatccatcgc cagggctatc cgttcatcgc ggccttcgcc gtgattacgc ttttcctcgg
      901 ctatttctcg tcgatcctgt tctggatcgg cctgatcctg accgcatggt gcgcctattt
      961 cttccgcgat cccgaacggg tgacgccgct ggacgaccgg ctggtggtca gccccgccga
     1021 tggcgtcatc tcggcggtcg gccctgccgc gccgccgcgc gaacttggcc ttggcgcggc
     1081 cgaaatgatc cgcatttccg tcttcatgga tgttttttcc tgccatgtta accgcgcccc
     1141 ggtgcgcggg cgcatcaccc gcatcgagca ccgtcccgga aaattcctca acgccgaact
     1201 ggacaaggcg agcgctgaaa acgaacgcaa cggcctcgtc atcgaaagcc cgaacggcac
     1261 tgtcgcggcg gtgcagatcg ccggcctcgt tgcgcgccgc atcgtctgct gggccaaggc
     1321 cggcggcacg ctggcggtgg gcgagcgctt cgggctgatc cgcttcggct cgcgcgtcga
     1381 tgtcttcctg ccgctcacgg ctacgccgcg cgttgccatc ggccagacgg cggtggccgg
     1441 cgagaccgtc cttgccgagt tcggcgctac gttggcggca ccgctcgtgc gggtttcctg
     1501 aaggatcgat ggtggcgcct ttcaagaagt tcgagccgca cggctccggc ggcccgcgca
     1561 tccgggaaat cccgctgcgc atgatgttcc ccaacctcat caccgtgctg gccatctgcg
     1621 ccggcctttc cggcatccgt ttcggcttcg agggccgttt cgagccggcg gtggtgatgg
     1681 tgctgttggc cgcctttctc gatggcgtcg acgggcggct tgcccgcctg ctgaaagcca
     1741 cctccaaatt cggcgcgcag atggattcgc tggccgatat cgtcaatttc ggcgttgcgc
     1801 ccgctttggt cctctacgcc tatctgctcg accgggccgg ctcgccgggc tggatcgcca
     1861 cgctgttgtt cgccatcgcc tgcggcatgc gcctggcgcg cttcaacgtg ctggacgagg
     1921 ataccgagca gcccgcctgg caggccgagt atttcgtcgg cgtgccggcg cccgccggcg
     1981 cggtgctggt gatgctgccg ctctatctgt ccttcctcgg ccttgagccg acaaagacca
     2041 cggctctcgt cagcagcggc ttcaccgttc tggtcgcctt cctgctggtc agccggctac
     2101 cggtctattc cggcaagagc ctgaaaattc ccggcgaccg gatgcttttc gtcatccttg
     2161 ccgtcgcgct ctatatcctg ctgctggtca gctatccctg gtacacgctt accgcttcgg
     2221 tcgccgtcta tctggttttc ctgccgttca gcatgcgggc ctattcccgc cgcgcgttgc
     2281 gcgagggcaa tgcgtccctg cgcccggatg tcgacgagcg gtctcccgaa tagacgccgg
     2341 cctgcggtct caaattcgca ccaccagcct gtcgagcgcc agcttgcgtg ctgatgtgta
     2401 gtccgtcttg ccggaaccca gcaccgggat ttcccccact ttgacgatat cgtgcggcac
     2461 catcagttcg ccgacgccgg cctgcttgcc tgcccggcgc agggtttcag ggtcggcggc
     2521 gtccgccgtg gtgacaagca cgatgcgttc gccgcgcttc ttgtccggta tcgccaccgc
     2581 tgcat
//
LOCUS       DASYCS010000051         6200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2909436,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000051 DASYCS010000000
VERSION     DASYCS010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 6200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6200
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2909436"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..492
                     /locus_tag="VGM46_01990"
     CDS             40..492
                     /locus_tag="VGM46_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018426441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY4191391.1"
                     /translation="MTGATQAANTNDSQGDCGDDLARIGEMAKIFGVTLRTLRFYEDK
                     GLLNPKREGSTRLYTRRDRARLKLILLGRKIGFSLRDVKQMIDLYDPTGSNARQLKLT
                     LEKSEKQFVRLQKQRMVIDEAIAELDGMMNSVRAMLVDRSAEKNRAAG"
     gene            722..2500
                     /locus_tag="VGM46_01995"
     CDS             722..2500
                     /locus_tag="VGM46_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018426442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEY4191392.1"
                     /translation="MPIYTAPVRDTLFVLNEMLGYERYANLPGFSDATPDVLEAILAE
                     GARLAENVMLPLNRIGDIEGCGRHDDGSVATPKGFRQAFEQYCEGGWMGLAAPAEFGG
                     QGLPYAVHSAVAEYMVSANMALMMYPGLTQGAIAAIVTHGSDAQKASFLPKMIEGAWT
                     GTMNLTEPHCGTDLGLLRTKAVPNGDGTYSISGQKIFISAGEHDMAGNIVHLVLARIE
                     GAPEGTKGISLFIVPKFKLDADGNAGERNAVSCGSLEEKMGIHGNATCAMNYDEAQGA
                     LLGEANGGLRAMFTMMNEARLGVGLQGQAISEIAYQNAVAYARERLQGRSLTDPKNKE
                     GKADPIIVHPDIRRALMTMKAFNEAGRALVLWTALKSDVAHRSGDENDRQASDDLLGL
                     MTPIVKGVLTDKGFDHAVMAQQVFGGHGYIEEHGMSQFVRDARIAMIYEGANGIQALD
                     LVGRKLALNGGRAVQAFFKEVGEFCEENRANEKMSPFTKGLKKGLNDLQAATLWLMQN
                     GMKNPDNAGAASTDYMHLFGLVALGYMWAQMAKTALAKADEAFYANKLVTARFFMERA
                     MPETAAHLARLSSGAETMMALPAEAF"
     gene            2513..3682
                     /locus_tag="VGM46_02000"
     CDS             2513..3682
                     /locus_tag="VGM46_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006336045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY4191393.1"
                     /translation="MTAPAEILSLPKPGWAGEDVAMLYDMAHRFMAEEIAPRYDEFEK
                     NEMVDRAAWEKAGAAGLLCASMPEEYGGAGGSFAHESAIIEAIGHVGVDGFGIGLHNA
                     IVAPYILHYGSQEQKAKWLPKLATGELIGAIAMTEPGAGSDLQGVKTRAEKDGNHYRI
                     NGSKTFITNGQLANLIIIVAKTDPEQGAKGTSLIVIETGGLDGFQRGRNLDKIGLKAN
                     DTSELFFNDMRVPTANLLGTEEGQGFVQLMQQLPQERLMIGTTAIAMAERALALTIDY
                     VKERKAFGKPILDFQNTQFKLAELKTEITIGRVFYNDCVARHLAGGLDPVTASMAKYW
                     LSDLQGKVVDECLQLHGGYGYMNEYPIARMYRDARVQRIYGGTNEIMKLLIGRSL"
     gene            3712..4920
                     /locus_tag="VGM46_02005"
     CDS             3712..4920
                     /locus_tag="VGM46_02005"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019863123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEY4191394.1"
                     /translation="MTEAYIYDAVRTPRGRGKKDGALHEVPAVRLAARTLEAIRDRNG
                     LDTGTVDDIIFGCVDPVGEAGAVIPRAAAFEAGYDTRAPGMQISRFCASGLDAVNLGA
                     AKIVQGADDIVIAGGVESMSRVGMGAAGGAWFMDPSVGFPGWFVPQGISADLIATKYG
                     FSRDDVDAYAVESQKRAAHAWEKGWFKNSVIPVKDQNGLTILDHDEHMRPSTDMQSLA
                     ALNPSFVMPGEMGGFDAVAVQKHPEVEEVIHVHHAGNSSGIVDGAAAVLLGSRKAGKA
                     MGLKPRARIRTFANIGSEPVLMLTGPVDVTEKLLKRAKMKLSDIDLFELNEAFAAVVL
                     RYMQAFDISHDKINVNGGAIAMGHPLGATGAIILGTVLDELERRDLNTALVTLCIGAG
                     MGTATIIERV"
     gene            4930..>6200
                     /locus_tag="VGM46_02010"
     CDS             4930..>6200
                     /locus_tag="VGM46_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015319307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY4191395.1"
                     /translation="MTYTNFTLDTDADGIALVTWDMPDRSMNVFTEQVMHELDSIVER
                     VTGDTAIKGVVITSGKETFSGGADIAMLQKMLQTFAAEKAAGVEKATQKLFETAGFMR
                     GLFRKLETCGKPWVSAINGTCMGGAFEMSLACHGRVAANSDKVKMALPEVKIGIFPGA
                     GGTQRVPRLTDQQQALQMLTSGQALTPQKAKSMGLIHEVVEPAKLVEAAKAMIRNGLK
                     PVQPWDEKGFKLPGGPVYSAAGANLWPPAIAILRRETHGNYPAATAILKCVYEGLLVP
                     FDTALRIEQRYFTEIMQTKEAAAMIRSLFISLQELNKGARRPEGVPETKFKKIGVLGA
                     GFMGAGIAYVTAKAGIPVVLLDRDMSAAEKGKAHSEGLIDGLIKKGRATQQDKEKLLA
                     LITPTADYAGLAGCDLVVEAVFEDSGVKKAAT"
BASE COUNT         1242 a         1919 c         1989 g         1050 t
ORIGIN      
        1 cgcatctttg catttttgag ggcaataacc atggaaccga tgacaggcgc cacgcaggcg
       61 gccaatacaa acgattcgca aggcgattgc ggtgacgatc ttgcccgtat cggcgagatg
      121 gcgaagattt tcggcgtgac cctgcgcacc ttgcgcttct atgaggacaa aggcctgctc
      181 aacccgaagc gggaaggctc gacgcgcctc tacacgcgcc gcgacagggc caggctgaag
      241 cttatcctgc tcgggcgcaa gatcgggttc tcgctgcgcg acgtaaagca gatgatcgac
      301 ctttacgatc caaccggctc caacgcccgg cagctcaagc tgacgctcga aaaatccgag
      361 aaacagtttg tgcgcctgca aaagcagcgg atggtgatcg acgaggccat cgccgaactc
      421 gatggcatga tgaattccgt gcgcgccatg ctggtggacc gctcggcgga aaagaaccgc
      481 gcggcgggct gaccgcgcat cttccatttc cggttccgtt cccggcaccg ttcgctgggc
      541 cattccggtc aggcgcggcc gcgtagctga aaattaaagc ccacccaaat attgacgttt
      601 acgcgaacgt caatatttgt taggtctgga tctgtcaggt ccggctcgac agcggcgccc
      661 attctattca cgataccgtt ccattcacgc tggctctcgc gccacaaggt tggaggaaga
      721 catgccgatc tacacggcgc cggtgcgcga tacgcttttc gtgctgaatg aaatgctggg
      781 ctacgagcgc tacgccaacc tgcccggttt ctccgacgcc acgccggatg tgcttgaggc
      841 gatcctcgcc gagggcgcca ggcttgccga aaacgtcatg ctgccgctca accgcatcgg
      901 cgacatcgaa ggctgtggcc gccacgatga cggctcggtg gcgacgccga agggtttcag
      961 gcaggcgttc gagcaatatt gcgagggcgg ctggatgggg cttgccgcgc cggcggaatt
     1021 cggcggccag ggcctgccct atgcggtgca ttcggcggtt gccgaatata tggtctcggc
     1081 caacatggcg ctgatgatgt atccgggcct gacgcagggc gcgatcgcgg ccatcgtcac
     1141 ccatggcagc gacgcgcaga aagcttcatt cctcccgaag atgatcgagg gtgcatggac
     1201 cggcaccatg aacctgacgg agccgcattg cggcaccgat cttggcttgc tgcgcaccaa
     1261 ggcggtgcca aacggcgacg gcacgtacag catctccggc cagaagatat tcatctcggc
     1321 cggcgagcac gacatggccg gcaacatcgt gcatctggtg ctggcccgca tcgaaggcgc
     1381 gccggaaggc accaagggca tttcgctgtt catcgtgccg aaattcaagc tcgacgccga
     1441 cggcaatgcg ggcgagcgca acgcggtttc ctgcggttct cttgaggaaa agatgggcat
     1501 tcacggcaat gccacctgcg ccatgaatta cgacgaggcg caaggcgcgc tgctgggcga
     1561 ggcgaatggc ggcctgagag ccatgttcac gatgatgaac gaggcgcgcc ttggcgtcgg
     1621 cctacagggt caggcgatct cggaaatcgc ctatcagaac gccgttgcct atgccaggga
     1681 gcgcttgcag ggccgttcgc tgaccgaccc gaagaacaag gaaggcaagg ccgatccgat
     1741 catcgtccac cccgacatcc gccgcgcgct gatgaccatg aaggcgttca acgaagccgg
     1801 ccgcgcgctg gtgttgtgga cggcgctgaa atccgacgtg gcgcaccgct cgggcgacga
     1861 aaacgaccgg caggcatcgg acgacctgct cggcctgatg acgccgatcg tcaagggcgt
     1921 gctgaccgac aagggtttcg accatgcggt gatggcgcag caggtgttcg gcggccatgg
     1981 ctatatcgag gaacatggca tgagccagtt cgtgcgcgat gcccgcatcg ccatgatcta
     2041 cgagggggcc aacggcattc aggcgctcga tctcgtcggg cgcaagctgg cgctgaacgg
     2101 cggccgcgcc gtgcaggcct tcttcaagga ggtgggcgag ttctgcgagg aaaaccgcgc
     2161 gaacgagaag atgtcgccct tcaccaaggg gctgaagaag gggctgaacg atttgcaggc
     2221 cgcgacgctt tggctgatgc agaacggcat gaagaacccc gacaatgcgg gtgcggcctc
     2281 caccgactac atgcatctct tcggcctggt ggcgcttggc tatatgtggg cccagatggc
     2341 caaaacagcg ctggccaagg cggatgaggc tttctacgcc aacaagcttg tcacggcgcg
     2401 cttcttcatg gagcgtgcga tgccggaaac ggcggcgcac ctggcgcgcc tgtccagcgg
     2461 ggccgagacg atgatggcgc tgccggcgga ggccttttaa gggatcgatg cgatgactgc
     2521 tccggctgaa atcctgtcgc tgccgaagcc cggctgggcc ggcgaggatg tcgccatgct
     2581 ctacgacatg gcacaccggt tcatggccga ggaaatcgcg ccgcgctacg acgaattcga
     2641 aaagaacgag atggtagacc gcgcggcatg ggagaaggcg ggcgccgccg gcctgctctg
     2701 cgcctccatg ccggaagaat atggcggcgc tggcggcagc ttcgcccatg aaagcgccat
     2761 catcgaggcc atcggccatg ttggcgtcga cggtttcggc atcgggctgc acaacgccat
     2821 cgtcgcgccc tacattctcc actacggctc gcaggagcaa aaggcgaagt ggctgccgaa
     2881 gcttgccacc ggcgaactga tcggcgccat cgccatgacg gagccgggcg ccggttccga
     2941 tctgcaaggc gtgaaaacgc gcgccgaaaa agacggcaac cactaccgaa tcaacggctc
     3001 gaaaaccttc atcaccaatg gccagcttgc caatctgatt atcatcgtcg ccaagaccga
     3061 ccccgaacag ggtgcgaaag gcacttcgct gatcgtcatc gagacgggcg gcctcgacgg
     3121 cttccagcgc ggccgcaacc tcgacaagat cgggctgaag gccaacgaca cgtccgagct
     3181 tttcttcaac gacatgcgcg tgccgaccgc caatctgctt ggcaccgaag aaggccaggg
     3241 cttcgtccag ctgatgcagc aactgccgca ggagcggctg atgatcggca ccacggcaat
     3301 cgccatggcg gagcgcgcgc tggcgcttac catcgactac gtcaaggagc gcaaggcttt
     3361 cggcaagccg atcctcgatt tccagaacac ccagttcaag ctggccgaac tgaaaaccga
     3421 aatcaccatc ggccgggttt tctacaatga ttgcgtggcg cgccacctcg ccggcggcct
     3481 cgatcccgtc accgcgtcga tggcgaagta ctggctttcc gacctacagg gaaaagtcgt
     3541 cgacgaatgc cttcaattgc atggcggcta tggttacatg aatgaatatc cgattgcgcg
     3601 catgtaccgt gatgcgcgcg tgcagcgcat ttacggcggc accaacgaga tcatgaaatt
     3661 gctgatcggc cgctcgctct agcgccgacc cgaaacctaa ggagtcgaga aatgactgag
     3721 gcttacatct acgacgccgt acgcacgccg cgcggacgcg gcaagaagga tggcgcgctg
     3781 cacgaagtgc ccgcggtgcg gctggcggcg cgcacgctgg aggcgatccg cgaccgcaac
     3841 ggcctcgata caggcacggt cgatgacatc atcttcggct gcgtcgatcc cgttggcgag
     3901 gctggcgcgg tgatcccgcg cgccgccgcc ttcgaggccg gctacgacac ccgagcgccg
     3961 ggcatgcaga tctcgcgctt ttgcgcctcg ggcctcgacg ccgtcaacct gggtgctgcc
     4021 aagatcgtgc agggcgccga cgacatcgtg attgccggcg gcgtcgaatc gatgagccgc
     4081 gtcggcatgg gcgcggccgg cggcgcatgg ttcatggacc cttcggtcgg ctttccgggc
     4141 tggttcgtgc cgcagggcat ttccgccgat ctgatcgcca ccaaatacgg cttttcgcgc
     4201 gacgacgtcg acgcctatgc ggtggaaagc cagaagcgcg ccgcccatgc ctgggaaaag
     4261 ggctggttca agaactcggt catcccggtc aaggaccaga acggcctgac catcctcgac
     4321 catgacgagc atatgcgccc ttccaccgac atgcagtcgc tggccgcgct caacccgtcc
     4381 ttcgtcatgc ccggcgaaat gggtggcttt gacgcggttg ccgtgcagaa gcacccggaa
     4441 gtggaggaag tgatccacgt tcaccacgcc ggcaattcat ccggcatcgt tgatggcgcc
     4501 gccgccgtgc tgctcggctc ccggaaggcc ggcaaggcga tggggctgaa gccgcgcgcg
     4561 cgcatccgca ccttcgccaa tatcggctcg gaaccggtgc tgatgctgac cggccccgtc
     4621 gatgtgacgg aaaagctgtt gaagcgcgcc aagatgaagc tgtccgacat cgacctgttc
     4681 gagttgaacg aggctttcgc cgccgtggtg ctgcgctaca tgcaggcttt cgacatctcg
     4741 cacgacaaga tcaacgtcaa tggcggcgcc atcgccatgg gccatccgct cggcgcgacc
     4801 ggggcgatca tcctcggcac cgtgctggac gagttggagc ggcgcgacct gaacaccgcg
     4861 ctggtgacgc tttgcatcgg cgccggcatg ggcaccgcaa cgatcatcga acgcgtttga
     4921 ggggcaggaa tgacctacac caactttacc ctcgataccg acgctgacgg cattgcgctt
     4981 gtcacctggg acatgcccga ccgttcgatg aacgtcttca ccgagcaggt gatgcacgaa
     5041 ctggattcca tcgtcgagcg cgtgaccggc gacaccgcca tcaagggcgt ggtgatcact
     5101 tccggtaagg agacattctc cggcggcgcg gacatcgcca tgctccagaa gatgctgcaa
     5161 accttcgccg ccgaaaaggc cgccggcgtc gagaaggcga cacaaaagct gttcgagacg
     5221 gcaggcttca tgaggggctt gtttcgcaag cttgaaacct gcggcaagcc gtgggtctcg
     5281 gccatcaacg gcacctgcat gggcggcgcg ttcgagatgt cgctcgcctg ccatggcagg
     5341 gtggccgcca acagcgacaa ggtcaagatg gcgctgccgg aagtgaagat cggcatcttt
     5401 cccggcgcag gcggcaccca gcgtgtgccg cgcctgaccg atcagcagca ggccttgcag
     5461 atgctgactt ccggccaggc gctgacgccg caaaaggcga aatcgatggg gcttatccat
     5521 gaagtcgtgg agccggcaaa gctggtcgag gcggccaagg cgatgatccg caacggcttg
     5581 aaaccggttc agccgtggga cgaaaagggc ttcaagctgc cgggcggccc ggtctattcg
     5641 gcggcgggcg ccaatctgtg gccgccggca atcgccatcc tgcgccgcga aacccatggc
     5701 aattatccgg ccgcgaccgc catcctgaaa tgcgtctatg agggcctgct ggtgccgttc
     5761 gacaccgcac ttcgcatcga gcagcgctat ttcaccgaga tcatgcagac gaaagaagcg
     5821 gcggcgatga tccgctcgct gttcatctcg ctacaggaac tgaacaaggg cgcgcggcgt
     5881 ccggagggcg tgccggaaac gaagttcaag aaaatcggcg ttctgggcgc cggcttcatg
     5941 ggcgcgggca tcgcctatgt cactgccaag gccggcattc cggtggtgct gctcgaccgc
     6001 gacatgtcgg cggccgaaaa gggcaaggcg cattcggaag gcttgatcga cggcctgatc
     6061 aagaagggcc gcgccaccca gcaggacaag gaaaagctgc tggcgctgat cacgccgacg
     6121 gccgactacg ccgggcttgc cggctgcgac ctcgtcgtcg aggcggtgtt cgaggattcg
     6181 ggcgtcaaga aggccgccac
//
LOCUS       DASYCS010000052         3007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_736163,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000052 DASYCS010000000
VERSION     DASYCS010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736163"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..143)
                     /locus_tag="VGM46_02015"
     CDS             complement(<1..143)
                     /locus_tag="VGM46_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEY4191396.1"
                     /translation="MSSRSCLSIVLAAGEGTRMKSALPKVLHQVAGLPLVAHVVKAAK
                     ASGA"
     gene            complement(253..999)
                     /locus_tag="VGM46_02020"
     CDS             complement(253..999)
                     /locus_tag="VGM46_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019858381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HEY4191397.1"
                     /translation="MALDIGYVSAVAAGALSFLSPCVLPLVPPYLCYMAGVSVDDFRG
                     DSGLAARTGARTALLASSVAFVLGFSTVFVALGAGASTIGHFLRVWQEPLAMAAGVLI
                     ILMGLNFLGILRLPFLSREARFQSEGKPASAVAAYVMGLAFAFGWTPCIGPVLGPILT
                     LAGGRETVGEGALLLAAYSFGLGLPFLVAALFSGAFMRFLGKFRIHLGRVEKAIGALL
                     VIAGVLFLTGGVQSASYWLLENFPVLGRLG"
     gene            complement(1309..1512)
                     /locus_tag="VGM46_02025"
     CDS             complement(1309..1512)
                     /locus_tag="VGM46_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191398.1"
                     /translation="MRQTYTRRRTETLDYLQAMLGQLRTMAEAERCDMLTYLIEMAYV
                     EASDIIRGERPARVLQDGRKNVA"
     gene            1806..>3007
                     /gene="topA"
                     /locus_tag="VGM46_02030"
     CDS             1806..>3007
                     /gene="topA"
                     /locus_tag="VGM46_02030"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEY4191399.1"
                     /translation="MDVVVVESPAKAKTINKYLGKNYKVLASFGHVRDLPAKDGSVRP
                     DEDFAMSWAVDGPSAKRLTEIANAVKSADGLILATDPDREGEAISWHVLEVLKQKRAL
                     KDKPVSRVVFNAITKQSVLDAMANPRQIDAPLVDAYLARRALDYLVGFTLSPVLWRKL
                     PGARSAGRVQSVALRLVCDRESEIERFIREEYWQIAAILGTPRKDSFEARLTAFEGKK
                     LQKLDIKNKAQADDIKAMLEGAAFKAQSVEAKPTKRNPGPPFTTSTLQQAASSRLGFS
                     ANRTMQVAQRLYEGMDIGGETAGLITYMRTDGVQMAPEAIDAARDAIAKEFGPKYLPE
                     KPRQYTAKAKNAQEAHEAIRPTDFMRTPASVRQYLDSDQMRLYELIWKRAIASQMQPA
                     EIERTTVE"
BASE COUNT          643 a          973 c          906 g          485 t
ORIGIN      
        1 gcgcccgatg cctttgccgc cttgacgacg tgggcgacga gcggcaggcc ggcgacctgg
       61 tgcagcactt tcggcagcgc gcttttcatg cgggtgccct cgccggcggc gaggacgatg
      121 gacaggcagg aacgcgagga catgggaaga acaccgatga tgaaggagat gaaggccgat
      181 atctatgtac tatcggtgtc gtcctacgcc aatgcggtta aattccggtg atgccagcgc
      241 aagtcgggcg gatcagccca gcctgcccag caccgggaaa ttctccagga gccagtagga
      301 tgcgctctgc acgccgccgg tgaggaacag cacgccggca atgaccagca acgccccgat
      361 ggccttttcg acgcggccga gatggatgcg gaacttgcca aggaagcgca tgaaggcgcc
      421 ggaaaaaagc gcggcgacaa ggaacggaag gccaaggccg aacgaatagg cggccagcag
      481 cagcgcgccc tcgcccaccg tctcgcgccc gccggccaac gtcaggatcg gccccaggac
      541 cgggccgatg cacggcgtcc agccgaaggc gaaggccagc cccatgacat aggcggcgac
      601 cgcgctggcc ggcttgccct cgctttggaa gcgcgcctcg cgcgacagga acggaagccg
      661 caggatgccc aggaaattca gccccatcag tatgatgagc acgccggccg ccatcgccag
      721 cggctcctgc cagacgcgca ggaaatggcc gatggtggaa gcgcccgccc ccagcgccac
      781 gaacacggtc gagaaaccga gcacgaaggc gacggacgac gccagcagcg cggtgcgcgc
      841 gccggtgcgc gccgcaaggc cgctatcgcc acggaaatca tcgaccgaaa caccggccat
      901 gtagcacagg taaggcggca ccagcggcaa cacgcaaggc gacaggaagg acaaagcccc
      961 cgccgcgacc gcgctgacat agccgatatc cagtgccatt ttttcgtctc cagcctgccg
     1021 tggctcttaa cggcaggccg gttcggcttc cagtcacagt tgcgtggcta atcgtcactg
     1081 aacggggtga acggatgttt ctggtcggtc catggatttg atgggggccc atggatcttc
     1141 gcgataactg gccatcgatt ttcgccatag gccaagcgac ggacatccaa gagccagatg
     1201 tcttcctgtg aggcgagccg ttccgcgacc ggtcctttca gccgctacct cgcgtgtaaa
     1261 gagcatggac cgtacgcgta tagagagtat ccgcgtggag cctgccagct atgccacgtt
     1321 cttgcgacca tcctgaagga cgcgcgccgg gcgctcgccg cggatgatgt cgctggcctc
     1381 cacataagcc atctcgataa ggtaggtcag catgtcacag cgttccgctt cggccatcgt
     1441 gcgcagttga ccgagcatcg cctgaagata atcgagtgtc tccgtccgcc gtctcgtgta
     1501 ggtttgccgc atcagactta accatcgcta tttactagcg acgcctccgc aagtcagaat
     1561 ttacggcggc caagccaagc catgaaccgg gccgccttga attcacaatg atctatacta
     1621 cttatccaga attgcaagaa ttttttccca caaccaccgg atgcatatcg atagccgcct
     1681 gcccattttg gccctcgccc cttgaccgaa agggaaagcg cggccatgtg atcgaccgtt
     1741 ccaacgaaat ttcgtcgcgc gcagcggaat tcttctgccg cccatcttga agaatatggt
     1801 cacccatgga cgtcgtcgtc gtagaatcgc cggccaaagc gaagacaatc aacaaatatc
     1861 tgggcaagaa ctataaggtt ctggcctcgt tcggccatgt ccgcgacctg ccggccaagg
     1921 acggctcggt gcgtcccgac gaagacttcg ccatgtcgtg ggccgtcgat ggcccgtccg
     1981 ccaagcggct caccgaaatc gccaacgccg tcaaaagcgc tgacggcctg atcctcgcca
     2041 ccgatccgga tcgcgaggga gaggccattt cctggcatgt gctcgaagtg ttgaagcaga
     2101 agcgcgctct gaaggacaag ccggtcagcc gcgtcgtctt caacgccatc accaagcagt
     2161 cggtgctgga cgcgatggcc aatccgcgcc agatcgacgc accgctggtc gatgcctatc
     2221 tggcgcggcg cgcgctcgac tatctggtcg gcttcaccct ttcgcccgtg ctgtggcgca
     2281 agctgcccgg tgcgcgttcc gccggccgcg tgcagtccgt cgcgctgcgg ctggtgtgcg
     2341 accgggaatc cgagatcgag cgcttcatcc gcgaagaata ctggcagatc gccgctattc
     2401 tcggcacgcc gcgcaaggac agcttcgaag cccgactgac ggctttcgag ggcaagaaac
     2461 tgcaaaagct ggacatcaag aacaaggcgc aggccgacga catcaaggcg atgctggagg
     2521 gcgccgcctt caaggcgcag tcggtggaag ccaagccgac caagcgcaat cctggcccgc
     2581 ccttcaccac ttcaacgctg caacaggcgg cctcgtcgcg tctcggcttt tcggccaacc
     2641 gcaccatgca ggtggcgcag cgcttgtatg aaggcatgga cattggcggc gaaaccgccg
     2701 gcctgatcac ttatatgcgt accgacggcg tgcagatggc gcccgaggcg atcgacgctg
     2761 cccgcgacgc catcgccaag gaattcggcc cgaaatacct gccggaaaag ccgcgccaat
     2821 acaccgccaa ggccaagaac gcccaggaag cacacgaagc catccgcccg acggatttca
     2881 tgcgcacccc ggcatcagtc aggcaatatc tcgattccga ccagatgcgg ctctatgagc
     2941 tgatctggaa gcgcgccatc gccagccaga tgcagccggc cgaaatcgag cgcaccaccg
     3001 tcgagat
//
LOCUS       DASYCS010000053         2241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4360194,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000053 DASYCS010000000
VERSION     DASYCS010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4360194"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            526..1551
                     /locus_tag="VGM46_02035"
     CDS             526..1551
                     /locus_tag="VGM46_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018446020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY4191400.1"
                     /translation="MRPTVHDVAGEAGVSLATVDRVLNKRAGVRAATVARVEAAIVRL
                     GFVRDIAAANLAKQRVYHLVFIIPDGPNSFMRALERELEQVRSNSVLDRTEIRVVKVP
                     AFDSTALVAALESLDPATISGLALVATETVGVRDGVARLRREGVAVVTMVSDMPASGR
                     DHFVGIDNVAAGRTAAGLIGRFAGQRRGKVALVAGSMLVRDHVERRMGFDQVIQAEFP
                     ALECLPVLEGRDDAEIVRELLAQCLEREPNIVGIYSIGAGNRGVIDVLQARPAYRDLV
                     VVAHELTDHSRKALREGLFDAVINQDAGHEIRSAIRLLKARIDGTRIVDGQESIRIEI
                     YLRDNLP"
     gene            1654..>2241
                     /locus_tag="VGM46_02040"
     CDS             1654..>2241
                     /locus_tag="VGM46_02040"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912894.1"
                     /note="catalyzes the interconversion of D-xylose to
                     D-xylulose; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HEY4191401.1"
                     /translation="MNSGFFGDIKPARYEGPDSTNPLAYRHYNKDEVVAGKRMEDHLR
                     FAVAYWHSFAWPGGDPFGGQTFDRPWFPKAGGADTMELARLKADIAFEMFAALDAPYF
                     CFHDADVRPEGRDFAESAARLDEIADYFAGKMAKTGTKLLWGTANLFSNRRFMAGAAT
                     NPDPDVFAYSAATVKACMDVTKRLNGENYVLWGGRE"
BASE COUNT          416 a          700 c          721 g          404 t
ORIGIN      
        1 tctcaacaag gcgctggccg aaatgacggc ggacggcacg ctggcaacca tgtcccagaa
       61 gtggttcggc gtcgatctca gcccgaaagg ctgagtaatt tttcgcgagg cggaccaaga
      121 ccgccgcccg gctggcgcaa attttcccgt gaccagccgg ccccggatca ggccgacgat
      181 cgaagatacc gatggggggg agccgtcggt cgatgccgat agcaggcacg gtcggcgcgt
      241 cataggtcgg ccgatcacgc ataacggcga tgaaacaggc aaggcttttc ctgcctgcct
      301 gtgatcctgg ccgcttcgcc gaggccgaag ctcgatcttc ccggcagacc atctaatcgc
      361 gccgctcgca acaccgccag ccgacccgca tcgcggcggc ttacccggcg cggcatagcc
      421 ggcctgacac aggcttccgg caaacattcg gtgccatcag cgccgacaga tattgcttta
      481 cgtacctcaa taccttcgct acggttcgtg tgagggggag tttcgatgcg tccgacggtt
      541 cacgatgtgg cgggggaggc cggcgtcagc ctggcgaccg ttgaccgcgt tttgaacaag
      601 cgggccgggg tcagggctgc gacggtcgcg cgggtggagg cggctatcgt caggctgggt
      661 ttcgtgcgcg acatcgccgc cgccaatctc gccaagcagc gcgtctatca tctcgtcttc
      721 atcataccgg acggaccgaa cagtttcatg cgcgcgctgg agcgcgagct tgagcaggtc
      781 aggtccaatt cggttctgga ccgtaccgaa atccgcgtcg tcaaggttcc ggcgttcgac
      841 agcacggccc ttgtcgcggc gctggaaagc ctcgatcccg caaccatttc cggcctggcg
      901 ctggtcgcca cggaaaccgt cggcgtgcgc gacggggtgg ccaggctgcg cagggaaggc
      961 gtggcggtcg tcaccatggt ttccgacatg ccggcgtcgg ggcgcgatca tttcgtcggc
     1021 atcgacaatg ttgcggccgg gcgcacggcg gccgggctta tcgggcgctt tgccgggcag
     1081 cgaaggggca aggtggcgct ggtcgccggc tcgatgctgg tgcgcgacca tgtcgagcgg
     1141 cgcatgggct tcgatcaggt catccaggcc gagtttccgg cgctggaatg cctgcccgtg
     1201 ctggaaggcc gcgacgacgc cgaaatcgtg cgcgaattgc tggcccaatg cctggagcgg
     1261 gagcctaaca tcgtcggcat ctacagcatc ggcgccggca atcgcggcgt gatcgatgtc
     1321 ttgcaggcga ggcctgcata ccgcgatctg gtcgtcgtcg cccatgaact gaccgatcac
     1381 tcccgcaagg cgctgcgcga aggcctgttc gacgccgtca tcaaccagga tgccggccat
     1441 gaaatccgca gcgcgatccg gcttttgaag gcgcgcatcg acggcaccag gatcgtcgac
     1501 ggacaggaaa gcatccgcat cgaaatttat cttcgcgaca acctgccctg acgggccaaa
     1561 tcgtgctagt cttgaggtac gtatatctaa actgaacgaa acggccttcc atggtcgatt
     1621 tcgcagcatt aaggaaaaag gaagtttact gccatgaaca gcggattttt cggcgacatc
     1681 aagcccgccc gatatgaagg cccagacagc accaatccgc tcgcctatcg ccattacaac
     1741 aaggatgagg tcgtcgcggg caagcgcatg gaagaccatc tgcgcttcgc cgtcgcctac
     1801 tggcattcct tcgcctggcc gggcggcgac ccgttcggcg gccagacctt cgaccggcca
     1861 tggtttccca aggcgggcgg cgccgacacg atggaactgg cccgactgaa ggccgatatc
     1921 gccttcgaga tgttcgcggc gctcgacgcc ccttatttct gcttccacga cgccgatgtg
     1981 cgaccggaag gccgtgactt cgctgaaagc gcggcgcgcc tcgacgagat cgccgactat
     2041 ttcgccggca agatggccaa aaccggcacc aaattgttgt ggggcaccgc caatctgttt
     2101 tccaaccgcc gtttcatggc gggcgcggcg accaatcccg acccggacgt gtttgcctat
     2161 tcggcggcga cggttaaggc ctgcatggat gtgaccaagc gtctgaacgg cgagaactac
     2221 gtgctgtggg gaggccgcga g
//
LOCUS       DASYCS010000054         8696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4939022,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000054 DASYCS010000000
VERSION     DASYCS010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 8696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8696
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4939022"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(338..1621)
                     /locus_tag="VGM46_02045"
     CDS             complement(338..1621)
                     /locus_tag="VGM46_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4191402.1"
                     /translation="MIVQSRLLGQRYAFVVVAVIFVCLLIAAGLRSAPAVMMLPMESQ
                     LGWGRDVVSLAAAVGIFLYGLAGPFAAALMERFGLRRILLGALALMAGSTALSLWMTQ
                     PWQMVATWGVFSGVGSGAVATVLGATIVNRWFKTNRGLVMGLMSASSATGMLVFLPVL
                     ASLAQWGGWRPVVMAVAIACACLLPLVWLLVPERPASIGLVRFGAEPDDVPPVSPAAQ
                     GNFFTHTLTTLRRAAGTRVFWYLFATFFVCGFTTNGLVGTHLIAFCGDMGIPEVKAAG
                     LLALMGVFDLIGTTLSGWLTDRFDPRKLLGMYYGIRGLSLIYLPMSGFSGTALTIFAV
                     LYGLDWIATVPPTLRLSNEAFGDRSGPIVFGWIVAGHQVGAATAAFFGGALRELQGSY
                     ELAFQIAGMTAIVAACLSLLIDTSRPAFEREPQPA"
     gene            1694..2308
                     /locus_tag="VGM46_02050"
     CDS             1694..2308
                     /locus_tag="VGM46_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY4191403.1"
                     /translation="MPRANNIDATISDSDQPAPRAAEKIMAAARDLFYSRGIRAIGVD
                     EIVRKAGVTKPSLYRSFPSKDELAASYLRRYDRDFWERFDQAVEAHPGDPRAQIAAFL
                     TRVGKRSQKPGYRGCGMTNAAVEYPETGHPARIVSEDNKKELRRRLREMAAAMGASDA
                     ATLGDGLLLLIEGAYISSQLFGQGGPAASVAKNADLMIAASLAD"
     gene            complement(2321..4372)
                     /locus_tag="VGM46_02055"
     CDS             complement(2321..4372)
                     /locus_tag="VGM46_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEY4191404.1"
                     /translation="MPDKAIDLAAHPLTAWNGPLGLPDFSRFGDDDFAPAFDAALKAH
                     AAEIDKIAGNPQPATIDNTLAALELGGEALDRVSSIFWLRAGAHTNPAIQALEREISP
                     RMSRHFSAISMNEKLFSRIDDLYQRRAGLKLDQETLRVLEKTWKGFVRSGAKLDAPGK
                     QRLAAINEELSSLGTKFGQNVLADESDWALFLDAADLAGLPDFLKASMAEAAEARGRQ
                     GSYAITLSRSIYEPFSTFSERRDLREAAWRAFTMRGQNGGASDNSAVVRDLLRLRAEK
                     AKLLGYASYAALKLDDTMAKTPKAVHDLLDPVWKKALEKAAGDQKELERLATEAGSNE
                     TFAAWDWRFYQEKLRAEKFAFDEAELKPYLQLDRVIAACFDVATRLFGLRFEEQHGIA
                     AWHPDARVFSVKNADGSERGLFLADYFARSSKRSGAWMSSLQSGYALGHGSKPVIYNI
                     MNFAKPPKGQPALLSMDEAKTLFHEFGHALHGMLTDVTWPSVSGTSVSRDFVELPSQL
                     YEHWLTVPAVLEKHALHVETGKPMPKALLEKMLAARTFGAGFATVEFTASALVDMAYH
                     ARADAPAEPLAFEAETLAALDMPDTIAMRHRTPHFGHVFSGDGYSAGYYSYMWSEVLD
                     ADAFAAFEETGDPFNAALAERLRENIYAAGGSKDPQELYTAFRGKMPSPEAMMTKRGL
                     V"
     gene            complement(4453..4845)
                     /locus_tag="VGM46_02060"
     CDS             complement(4453..4845)
                     /locus_tag="VGM46_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006201194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY4191405.1"
                     /translation="MAAWMSAPPCFAAEFIAGGAVIDRWYAALLAADRGGLSDLLADD
                     ARIRLDDLGIEQNKQEFLDSMDEWQASAKGAAIRHRVEKNENGVSTVIACYDFPGNDL
                     LMREVFAISGAVITAGAQARIADNCDSF"
     gene            complement(4899..5543)
                     /locus_tag="VGM46_02065"
     CDS             complement(4899..5543)
                     /locus_tag="VGM46_02065"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEY4191406.1"
                     /translation="MAHIPEHLLTGYRNFMTGRYPAESGRYSDLAREGQSPETMIIAC
                     SDSRSAPETIFDAGPGELFVLRNVGNLVPPCEPDGEFHSTSAALEFAVQALKVKNIVV
                     MGHGRCGGIRAALAQGSGPLAPGDFIGKWMSLIKPAANAVASATDMTASERQTALERI
                     SIRYSLANLRTFPKIKEREEKGELALYGAWFDISTGELWTMNRDTGDFERPVLD"
     gene            complement(5642..6523)
                     /gene="pdxY"
                     /locus_tag="VGM46_02070"
     CDS             complement(5642..6523)
                     /gene="pdxY"
                     /locus_tag="VGM46_02070"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HEY4191407.1"
                     /translation="MSTELAARRAVIVISSHVVRGTVGNRAAVFALETLGFPVWAVPT
                     VTLAWHPGHGPATRIVAKEEQFAALLADLERSPRLGEVGAVLSGYLGEASQAKAVAAL
                     VGAVKAKNPDALYVCDPVIGDSGGLYVPEATATAMRDRLVPIADIATPNRYELEWLAG
                     AKLPDLKAVIAAARDAGPATMLVTSAPAMMADSIGNLLVDASQAILAEHRVIAGPPNG
                     LGDLTAAVYLARLLSGQPPAKALQSTTAAVFEILARTARRGGDELQLETDAQSLAHPM
                     AMVQVRHFRQPGRDARA"
     gene            complement(6530..6784)
                     /locus_tag="VGM46_02075"
     CDS             complement(6530..6784)
                     /locus_tag="VGM46_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191408.1"
                     /translation="MPQRRNDDDGESRRILQRIAGESEPGGASFAVRKAKGALDRVSA
                     ADADRSDAIEYWGTRIGRVLGLLLAIGLMIWLVIFLTRGG"
     gene            6959..7597
                     /locus_tag="VGM46_02080"
     CDS             6959..7597
                     /locus_tag="VGM46_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018430192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEY4191409.1"
                     /translation="MFAASLPQFRIRRFLPTFLVMAFALPLLAGCGYNTIPTAEENAK
                     AAFSEVQNQYQRRADLIPNLVETVKGYASHEKDTLEAVVEARAKATQITITPETLKDP
                     EAFKKFQESQSGLTSALSRLLAVAENYPDLKANQNFLALQAQLEGTENRIAVARRDYI
                     QAVRDYNLTLRTFPSVIWAKLWFTANEPFPNFTAPEDKLEVPKIDFGTKQGG"
     gene            7720..8538
                     /locus_tag="VGM46_02085"
     CDS             7720..8538
                     /locus_tag="VGM46_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HEY4191410.1"
                     /translation="MPLALLLFLLTAFLPFHAFAADLPALTGRVVDNAGIIDAASKVL
                     LEQKLADFEKKGSDQIVVATIPTLDGEDIETYANRLFRFWKLGQAGENNGVLLLVAQN
                     DRKMRIEVGYGLEGTLTDLHTRLIIENDMVPAFRAGDFPGGIEKAVDDMVMVLEGNPE
                     ELEARGQRNQQESAGDIEDWIPILFVIIWATLFFGGFAMAILPPMFGQKIGPNKYRWL
                     GMTFNTARSSSRGGGWTSGGGGWSSGGGGGWSSGGGGFSGGGGSSGGGGSSGSW"
BASE COUNT         1649 a         2855 c         2760 g         1432 t
ORIGIN      
        1 gcacaagcct cggtcgaggc ccggaccttg gggctcatcg cccagccgca ctaccgttgt
       61 gcaaccgatc cggcaccgtc ccctccacgc tcatccggtt catgacccgt gttcccgcga
      121 gggagatggc gaggattatg ccggcaggcg gagagggtgt ggatggattt attttttgcg
      181 cgcttctgtc ccccgcgctg cctcggcgcg gcgcgcgagc cgcaacccct ctctcgtcat
      241 cccggaaact gcgtagagcg caacgacagg cggctgtccg ggatgacgaa ggtggggtct
      301 ccttaagcga gcaaggacta ctcccgccca aaatcgatca ggccggctgc ggctcgcgct
      361 cgaaggccgg gcggctggtg tcgatgagca gcgacaggca ggcggcgacg atggccgtca
      421 tgccggcgat ctggaacgcc agttcgtagc tgccttgcaa ttcgcgcagc gcaccgccga
      481 aaaaggctgc ggtggccgcg cccacctgat ggccggcgac gatccagcca aacacgatcg
      541 gacccgagcg gtcgccgaag gcttcgttcg acaggcgcag cgttggcggc accgtcgcaa
      601 tccagtcgag gccgtagagg acggcgaaga tggtcagcgc cgtgccggaa aagcccgaca
      661 tcggcaggta gatcagcgac aggccgcgaa tgccgtaata catcccaagc agcttgcgcg
      721 ggtcgaagcg gtcggtcagc cagccggaca gcgtcgtgcc gatcaggtcg aacacgccca
      781 tcagcgccag caggccggcg gctttcacct cgggaatgcc catgtcgccg caaaaggcga
      841 tgagatgggt gccgaccagc ccgttggtgg tgaagccgca gacgaaaaag gtggcaaaca
      901 gataccagaa cacccgcgtg cctgccgcgc ggcgcagcgt ggtaagcgta tgcgtgaaga
      961 aattgccctg ggctgccggc gaaaccggcg gcacatcgtc cggctccgcg ccgaaacgca
     1021 ccagcccgat cgaggccggc cgctccggca ccagcagcca gaccagcggc agcaggcagg
     1081 cgcaggcgat ggccaccgcc atgacgaccg gccgccagcc gccccactgc gcaaggcttg
     1141 ccagcaccgg caggaacacc agcatgccgg tggcgctgga agccgacatc aggcccatca
     1201 ccagcccgcg attggtcttg aaccagcggt tgacgatggt cgcgccaagc acggtcgcca
     1261 ccgcgccgga accgacgcct gaaaaaacgc cccatgtggc gaccatctgc cagggctgcg
     1321 tcatccacag gctgagcgcg gtcgagccgg ccatcagcgc cagcgcaccc aaaaggatgc
     1381 ggcggaggcc gaagcgctcc atcagcgcgg cggcgaaagg cccggccagc ccgtagagga
     1441 aaatgccgac ggcggcggcc agcgaaacca cgtcgcgccc ccagccaagc tgcgattcca
     1501 tcggcagcat catgacggcg ggcgccgagc gcaggccggc ggcgatcagc aggcagacga
     1561 aaatgacggc gacgacgaca aacgcgtagc gctggccaag caatcgagac tggactatca
     1621 tgttactgac cggtacgtta cgggaagcac cactatgcgt accggtcggt aacattgtca
     1681 agcgagagcg gaaatgccac gcgccaataa tattgatgca acgatcagcg atagcgatca
     1741 accggccccg cgcgccgccg aaaagatcat ggcggcggcg cgcgacctgt tctacagccg
     1801 cggcataagg gcgatcggcg tcgacgagat cgtgcgcaag gccggcgtca ccaagcccag
     1861 cctctatcgc agctttcctt ccaaggatga actcgccgcc tcctatctgc ggcgctacga
     1921 cagggacttc tgggagcgtt tcgaccaggc tgtcgaggcc catcccggcg atccgcgcgc
     1981 gcagatcgcg gcttttctca cccgcgtcgg caaacgttcg caaaagccgg ggtatcgcgg
     2041 ctgcggcatg accaatgcgg cggtcgaata tcccgaaacc ggccaccctg cccgcatcgt
     2101 cagcgaagac aacaagaagg agttgcgccg ccgcctgcgc gaaatggccg ccgcgatggg
     2161 cgcaagcgac gccgccacgc tgggcgacgg cctgcttttg ctgatcgagg gggcctatat
     2221 ctccagccag ctgttcggcc agggcggccc ggcggcctcg gtagccaaaa acgccgacct
     2281 gatgattgct gcgagtctgg cggattagcg cggcaggcgg ctacaccagc ccgcgctttg
     2341 tcatcatcgc ttcaggcgaa ggcatcttgc cgcgaaacgc cgtataaagc tcctgcgggt
     2401 ccttcgagcc gccggccgcg taaatgttct cgcgcagccg ctctgccagc gccgcattga
     2461 acgggtcgcc ggtttcctcg aaggccgcga aggcgtcggc gtcgagcact tccgaccaca
     2521 tgtaggaata atagccggct gaataaccgt cgccggaaaa cacatgcccg aaatgcgggg
     2581 tgcggtggcg catggcaatg gtgtcgggca tgtccagcgc ggcgagcgtc tcggcctcga
     2641 aggccagcgg ctccgccggg gcgtccgccc tggcgtgata ggccatgtcg accagcgccg
     2701 aagcggtgaa ctcgaccgtg gcgaagccgg ccccgaaggt gcgcgcggcc agcatctttt
     2761 ccagcaacgc cttgggcatc ggcttgccgg tctcgacatg cagcgcatgc ttttccagca
     2821 cggcgggcac cgttagccaa tgctcgtaaa gctgcgacgg cagttcgacg aaatcgcggc
     2881 tgaccgaagt tcccgaaacc gatggccagg taacgtcggt caacatgccg tgcagcgcat
     2941 ggccgaattc atggaacagc gtcttcgcct cgtccatcga cagcagcgcc ggctggcctt
     3001 tcggcggctt ggcgaaattc atgatgttgt agatgaccgg cttcgagccg tggccgagcg
     3061 catagccgga ttgcagcgaa ctcatccacg cgccggagcg cttcgaggat cgcgcgaaat
     3121 aatcggcgag gaaaaggccg cgttcggagc catcggcgtt tttcaccgaa aagacgcgcg
     3181 catccgggtg ccaggcggcg atgccatgct gctcctcgaa acgaagcccg aacagccgcg
     3241 tcgccacgtc gaaacaagcg gcgatgacgc ggtcgagctg aagatagggt ttcagttcgg
     3301 cctcgtcgaa agcaaacttc tcggcgcgca gcttctcctg gtagaaacgc cagtcccagg
     3361 cggcgaaggt ttcgttgctg cccgcttccg tcgccaggcg ctccagttcc ttctggtcgc
     3421 cggcagcctt ttccagcgcc ttcttccaga ccggatcgag cagatcgtgc accgctttcg
     3481 gcgtcttggc catggtgtcg tcaagcttca gcgcggcata ggaagcgtaa ccgagcagct
     3541 ttgccttttc ggcgcgcagc cgcaacaggt cgcgcacgac ggcgctgttg tcgctggcgc
     3601 cgccgttctg gccgcgcatg gtgaaggcgc gccacgccgc ctcgcgcaga tcgcgccgct
     3661 ccgagaaggt cgaaaacggc tcgtagatgg aacgcgacag cgtaatggcg tagctgcctt
     3721 ggcggccacg cgcctcggcg gcttcagcca tcgaagcctt gaggaaatcc ggcagcccgg
     3781 caaggtcggc cgcgtccagg aacagcgccc aatcgctctc gtcggccagc acgttctgcc
     3841 cgaacttcgt gccaagcgac gacagttcct cgttgatggc cgccagccgc tgcttgcccg
     3901 gcgcgtcgag cttggcgccg gaacgcacga agcctttcca ggtcttttcc agaacgcgca
     3961 gcgtctcttg atcgagcttc agcccggcgc ggcgctggta aagatcgtcg atgcgcgaaa
     4021 acagcttttc attcatcgat atcgccgaga aatgccgcga catccttggc gagatttcgc
     4081 gctccagcgc ctggatcgcc gggttcgtat gtgcgccggc ccgaagccag aagatcgatg
     4141 acacccggtc gagcgcctcg ccgccaagtt cgagcgcggc cagcgtattg tcgatggtgg
     4201 ccggctgcgg attgccggcg atcttgtcga tttcagccgc atgcgctttg agcgccgcat
     4261 cgaaagcggg cgcgaaatca tcgtcgccga agcgcgaaaa atccggcagg ccaagcgggc
     4321 cgttccatgc ggtgagcggg tgcgcggcaa ggtcgatggc tttatcgggc atgaatttct
     4381 ctcggcggat ggggatttac gacaggcggg cgaccatccc gatgtagggt gccgcccggc
     4441 ttcacgcaac ggctagaagg aatcgcagtt gtcggcgatc ctcgcttgcg cgccggcggt
     4501 gatgacggcg ccggaaatgg cgaacacttc ccgcatcaac aggtcgttgc cgggaaaatc
     4561 gtagcaggcg ataaccgtcg agacgccatt ttcgttcttt tcgacgcgat gtcggatggc
     4621 cgcgcccttg gccgacgcct gccactcgtc catcgaatcg agaaattcct gcttgttttg
     4681 ctcgatgccg agatcgtcga gcctgatgcg ggcatcatcg gccaggagat cggaaagccc
     4741 accacggtcg gcggccagca aggcggcgta ccagcggtcg ataacggccc cgccggcgat
     4801 gaattcagcg gcgaagcagg gcggcgcgga catccaggca gccatcgccg caagcagcag
     4861 aaaacgcgtt ttcatggcag gcgctcccgc cggctggttc agtcgaggac aggccgctcg
     4921 aagtcgccgg tatcccggtt catcgtccac aattcgccgg tcgagatatc gaaccacgcg
     4981 ccataaagcg caagttcacc cttctcttcc cgctccttga tctttgggaa cgtgcgaaga
     5041 ttagccagcg aataacggat cgatatccgc tccagcgccg tctggcgctc gctcgccgtc
     5101 atgtcggtgg cggaggcgac ggcattggcg gcgggcttga tcaggctcat ccacttgccg
     5161 atgaaatcgc ccggcgcaag cgggcctgag ccttgtgcga gagcggcgcg aatgccgccg
     5221 cagcggccat ggcccatcac cacgatgttc ttgaccttga gcgcctgcac cgcaaattcg
     5281 agcgccgccg aagtggagtg gaactcaccg tccggctcgc agggcggcac cagattgccg
     5341 acattgcgca agacgaacag ctcgcccggc ccggcatcga aaatcgtctc gggcgccgaa
     5401 cgcgaatccg agcaggcgat gatcatggtt tcgggcgact ggccttcacg ggcgagatcg
     5461 ctgtagcgcc cgctttcggc cggatagcgg ccagtcatga aattgcggta gccggtgagg
     5521 agatgttctg gaatgtgagc catcgtggga tgccattaac ggcaaagccc ggccgcgatc
     5581 aacgaaaatc gcaaccctgc ggcacaatct cccgcacggc ctttccgccg cgcgggaggc
     5641 ctcacgccct ggcgtcgcgc cccggctgcc ggaaatggcg cacctgcacc atggccatcg
     5701 gatgggccag gctttgcgca tcggtttcca gttgcagttc gtcgccgccg cgcctggccg
     5761 tgcgcgccaa aatctcgaac acggccgccg tggtcgattg cagcgccttg gccggcggct
     5821 ggcccgacaa cagccgcgcc agatagacgg cggccgtcaa atcgccaagt ccgttcggcg
     5881 gcccggcgat gacgcggtgt tcggccagta tcgcctggga cgcatcgacc agcagattgc
     5941 cgatgctgtc tgccatcatc gccggcgccg aggtgaccag catggttgcc ggcccggcat
     6001 cgcgcgccgc cgcaatcacc gccttgagat cgggcagctt cgcgcccgcc agccattcga
     6061 gttcgtaacg gttaggcgtg gcgatatcgg caatcggcac cagcctgtcg cgcatggcgg
     6121 tcgcggtcgc ttccggcacg taaagcccac ccgaatcgcc gatcaccgga tcgcagacat
     6181 agagggcgtc cgggtttttc gccttgaccg cgccgaccag cgcagcgaca gccttggcct
     6241 gcgaagcctc gcccaggtaa ccggaaagca ccgcgcccac ttcgccaagc cgcggcgaac
     6301 gctccaggtc ggccagcagc gccgcgaatt gctcttcctt cgccacgatg cgcgtcgccg
     6361 gaccgtggcc cggatgccag gcaagcgtga cggtgggcac cgcccatacc gggaagccca
     6421 gtgtttccag cgcaaatacg gcggcgcggt tgcccaccgt tccgcgcacg acatggctgg
     6481 aaatgacgat gaccgcgcgc cgcgccgcaa gttcagtgct catggatgtt catccgcccc
     6541 gcgtaagaaa gatgacgagc catatcatca ggccgatggc gagcaacagg ccgagcacgc
     6601 ggccgatgcg cgtaccccaa tattcgatgg cgtccgaacg gtcggcgtcg gcagccgaaa
     6661 cgcggtccag cgcgcccttg gccttgcgca ccgcaaacga tgcgccgccc ggttccgatt
     6721 cgcccgctat ccgctgaagg atgcggcggg attcgccatc gtcgtcattc cggcgctgcg
     6781 gcatgcggat atcctgtctg acggcaaagc ttagccggtt ccgcgaacgg ttgacagggt
     6841 ttggcccagt gatggtgcgc tttgctatag gtgcgaaaat gcggatattc gcggcccatt
     6901 ttcttcgcca gcgtttgtgc tacagattgg ccatccgatc cttttgaggg acccgcctat
     6961 gtttgccgcc agcctgcctc aattccgcat ccgccgtttt ctgccaacct ttctggtgat
     7021 ggccttcgcg ctgccgctgc ttgccggctg cggctacaac accattccga cggcggaaga
     7081 aaacgccaag gccgccttct cggaggtgca gaaccagtat cagcgccgcg ccgacctcat
     7141 ccccaatctg gtcgagacgg tcaaaggcta tgccagccat gaaaaggata cgctggaggc
     7201 ggtggtcgag gcgcgcgcca aggcgacgca gatcaccata acccccgaaa cgctgaaaga
     7261 cccggaagcc ttcaagaaat tccaggaaag ccagtcgggc ctgaccagcg cgctgtcgcg
     7321 gctgctcgcg gtcgccgaaa actatcccga cctcaaggcc aaccagaact tcctggcctt
     7381 gcaggcgcag ctggaaggca ccgaaaaccg cattgccgtg gcaaggcgcg attatatcca
     7441 ggcggtgcgc gactataatc tgacgctgcg caccttcccc tcggtgatct gggccaagct
     7501 atggttcacc gccaacgagc ctttcccgaa ctttacggcg cccgaagaca agctcgaagt
     7561 gccgaagatc gattttggca ccaagcaggg cgggtgatcc ttgccctgct gaaccagagc
     7621 ggttcgacct tgcgctcccc ggcacgcccc cgcagccggc cttcatgggc cggcaacccg
     7681 accttagcgc cgggtgtgcg aagcagtggc gttctggcga tgccgctcgc cctgttgctt
     7741 tttcttctta cggcgttcct gcccttccac gccttcgccg ccgacctgcc ggcgctgacc
     7801 ggccgcgtcg tcgacaatgc cggaatcatc gacgcggcca gcaaggtgct gcttgagcag
     7861 aagctggccg atttcgagaa aaaaggctcc gaccagatcg tcgtcgccac cattcccacg
     7921 ctcgatggcg aagatatcga gacctacgcc aacaggctgt tccgtttctg gaagctcggg
     7981 caggccggcg agaacaacgg cgtacttttg ctggtcgccc agaacgaccg caagatgcgc
     8041 atcgaagtcg gttacggcct cgaaggcacg ctgaccgacc tgcatacaag gctgatcatc
     8101 gaaaacgaca tggtgccggc ttttcgcgcc ggcgactttc ccggcggcat cgaaaaggcc
     8161 gtcgacgaca tggtcatggt gctggagggc aacccggagg aactggaagc gcgcgggcag
     8221 cgcaaccagc aggaaagcgc cggcgacatc gaggactgga tacccatcct gttcgtcatc
     8281 atctgggcaa cgctgttctt cggcggcttc gccatggcca tcctgccgcc gatgttcggc
     8341 cagaagatcg ggccgaacaa ataccgttgg ctgggcatga ccttcaacac cgcgcgctcc
     8401 tcgtcgcgcg gcggcggctg gacctccggc gggggtggct ggtcttcagg cggcggcggc
     8461 ggctggtcat ccgggggcgg cggcttttca ggcggcggcg gctcgtccgg cggcggcggc
     8521 tcttcgggaa gctggtgagg cgcagcatgg caagacatac catcagcccg caagaccacg
     8581 cccgcgtgac ggcagccatc aagaccgccg aacagaagac cgacggcgaa atctactgcg
     8641 taatggcgca tgccagcgac ggctatttct ttcccgccgc ccttgtttcg atcgtg
//
LOCUS       DASYCS010000055         4611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2329743,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000055 DASYCS010000000
VERSION     DASYCS010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2329743"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..913
                     /locus_tag="VGM46_02090"
     CDS             <1..913
                     /locus_tag="VGM46_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003527008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4191411.1"
                     /translation="TSATNPIELRRSIGYVIQQIGLFPNKTVADNICVVPDLLGWDRK
                     KARLRAAELLELVGMKPDEFMRRYPRELSGGQQQRVGVIRALAADPPVMLMDEPFGAI
                     DPISRESIQDEFLRMQKTLRKTIIFVSHDIDEAVKMADKIAIFRGGRLEQFASPERLL
                     ANPANDFIESFLGTDRALKRLKLVRVRDAMLAVPDPLRSNDPIARADELMRVSGTPAM
                     VMVDEAGRPAGLVASGQGGDSGTVADHVQPVRATVLTTSDLRNAVALMFANDMPVLPC
                     IDADGRLAGILTWRSIVKSLSAVEAPA"
     gene            910..1701
                     /locus_tag="VGM46_02095"
     CDS             910..1701
                     /locus_tag="VGM46_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4191412.1"
                     /translation="MNAAAANPIDATPRWPGARGASDTGGSRRGIVIIWLVVIAAIVL
                     WAWFSGMPAEILTYQDDITYLARQHIELVAWAGIFAILVGIPVGAVLSRPAFRNFSEL
                     VLQVFNLGTTIPTLAVLALSMTWLGIGTVPAIFGLWAATLLPIVRNTYTGLRAVPPHL
                     IEAARGMGMTPAQTFWRVELPNALFVIFGGIRTALAVCVGTAPLAFLIGAGGLGELIF
                     TGIALDELPMMLAGAIPTALIAVLTDFLVGRLQFYAVPRGINPLR"
     gene            1736..2626
                     /locus_tag="VGM46_02100"
     CDS             1736..2626
                     /locus_tag="VGM46_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017268912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY4191413.1"
                     /translation="MAIFSKLTTALISAGALLALAGTSMSATLTVGGKAFTEQLVISE
                     ITKQLLEEKGYTVDKKDGMGTKIVRAALENGEVDLYWEYTGTSLITFNKIKDKLSPEE
                     TYARVKQLDGEKGLVWLAPSKANNTYAMTIRKDNPKTNGMETVSDLAAAYNAGKPVVL
                     GTSAEFPKRPDGLPGLEQVYGFKAGRENVRPMDLGLAYKALGNGDLDVIASQATDGLI
                     AAMNLKVLKDDKGFFPDYAMTPVVRKEVLDANPDLKGILESLSAKLDDATMQHLNGEV
                     DVDKKTVEQVATDFLKQNKL"
     gene            2735..3625
                     /locus_tag="VGM46_02105"
     CDS             2735..3625
                     /locus_tag="VGM46_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017272967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY4191414.1"
                     /translation="MNIFSKLGAAVLSAAVVLSGWAAMAANLTVGGKDFTEQLVLAEM
                     TKQLLEAKGYTVDKKDGMGTSIVRAALESGEVDMYWEYTGTSLVTFNKITEHLSPEQT
                     YAKVKELDGAKGLVWLAPSKANNTYALAIRQNNPKTDGMKTYSDLAAAYRDGKEVLMG
                     ATAEFVKREDGLLGLQKMYDFKAGRANTRPLDVGLVYNALANGDVDVSTVGATDGRIA
                     AMHLILLKDDKSFFPNYALAPVIAKKTLDAHPDLQGLLESLSGKLDDATMQRLNGEVD
                     VEKKTVEDVATGYLKQAGLI"
     gene            3686..>4611
                     /locus_tag="VGM46_02110"
     CDS             3686..>4611
                     /locus_tag="VGM46_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008839240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY4191415.1"
                     /translation="MTTDYARLIDAETWAFINRINAFYPPETANLPIERNREIYDRMS
                     RAFHTAYPAGVSAQAGTVPLADHDVALRIYRKAGGPEAAVVLYFHGGGFILGGLDSHD
                     DICADICAGTGAIVISVDYRLAPEHPGAAAFDDAVAAFEWVGANYPQPIVLVGESAGG
                     NLAACVAHHTRGHALAAIGQVLIYPGLGGDEGQGSYVTHAEAPMLSTRDLDFYRAVRT
                     GEANVSDDPRYTPLADNDFSELPPTVIFTAQCDPLSSDGEAYRDRLLRGGGKARWQEE
                     EALPHGYLRARHTVGRARASFARIVEAIGMLG"
BASE COUNT          896 a         1405 c         1455 g          855 t
ORIGIN      
        1 caccagcgcc accaacccga tcgagttgcg ccgttccatc ggttacgtca tccagcagat
       61 cgggcttttc ccgaacaaga cggtggccga caacatctgc gtggtgccgg acctgctcgg
      121 ctgggaccgc aagaaggcgc ggctgcgcgc tgcggaactg ctggaactgg tcggcatgaa
      181 gccagatgaa tttatgcgcc gctatccgcg cgaactgtcg ggcgggcagc agcagcgcgt
      241 cggcgtaatc cgcgcattgg ctgccgatcc gccggtgatg ctgatggacg agccattcgg
      301 cgcgatcgat ccgatcagcc gcgaatccat ccaggacgaa ttcctgcgca tgcagaagac
      361 gctgcgcaaa accatcattt tcgtttcgca cgatatcgat gaagcggtga agatggccga
      421 caagatcgcc attttccggg gtgggcggct ggagcagttt gccagtcccg aaaggctgct
      481 ggcaaatcct gccaacgact tcatcgagag ttttctgggc acggatcgcg cgctgaagcg
      541 gctgaagctg gtcagggtac gcgacgccat gctggccgtg ccggacccgt tgcgcagcaa
      601 cgaccccatc gcgcgtgcag acgaactgat gcgtgtttcc ggcaccccgg caatggtgat
      661 ggtggatgag gcgggccgcc cggctggtct ggtcgccagc ggacaaggcg gagatagcgg
      721 aacggtcgcc gaccatgtcc agccggtgcg cgcgaccgtg ctgacgacat ccgatctccg
      781 caacgccgtt gccttgatgt ttgcaaacga catgccggtg ctgccctgca tcgatgcaga
      841 cggacggctt gccggcatcc tgacctggcg ctctatcgtc aagagcctgt cggctgtcga
      901 ggcgccggca tgaacgctgc cgcggccaac ccgatcgacg ccacgcctcg ctggccaggc
      961 gcacggggag cttccgacac aggcggctca cgtcgcggca ttgtgatcat ctggctggtg
     1021 gttatcgccg ctatagtgct gtgggcttgg ttcagcggca tgcccgctga aatcctgacc
     1081 tatcaggacg acatcaccta tctggcgcgt cagcatatcg aactcgtggc ctgggccggc
     1141 attttcgcca ttcttgtcgg cataccggtc ggcgcggtgc tgtcgcggcc ggccttccgc
     1201 aatttttcgg aactggtact tcaggtcttc aacctcggca ccaccatccc gacactcgcg
     1261 gtgcttgcct tgtcgatgac ctggcttggc atcggcaccg taccggccat cttcggcctg
     1321 tgggcagcga cgctgctgcc gatcgtgcgc aacacctata cgggcctgcg ggccgttccg
     1381 ccgcatctga tcgaggccgc gcgcggcatg ggcatgaccc ccgcgcagac attctggcgg
     1441 gtggagttgc caaacgcgct attcgtgatt ttcggcggca tccgcaccgc gcttgccgtc
     1501 tgcgtcggca cagcgccgct cgccttcctg atcggcgccg gcggcctcgg cgagttgatc
     1561 tttaccggta tcgcgcttga cgaactgccg atgatgcttg ccggcgccat tccgaccgcg
     1621 ctgatcgcgg tcctgaccga tttccttgtc ggccggctgc aattttacgc cgtgccgcgc
     1681 ggaataaacc cgcttcgata gaatgtgaaa caaacatcaa aaaaagggga aagacatggc
     1741 tattttttcg aaactgacca cggcgctgat ctcggcgggc gctttgctgg cgcttgccgg
     1801 aacgtcgatg tcggcaacgc tgacggtggg cggcaaggcg tttaccgagc aattggtcat
     1861 ctccgagatc accaagcaat tgctggagga gaagggctat acggtcgaca agaaggacgg
     1921 catgggcacc aagattgtcc gcgccgcgct ggagaatggc gaagtcgatc tctactggga
     1981 atataccggc acgtcgctga tcaccttcaa caagatcaag gacaagctgt cgccggagga
     2041 gacctatgcg cgggtcaagc agttggacgg cgaaaagggc ttggtctggc ttgctccgtc
     2101 caaggccaac aacacctatg cgatgaccat ccgcaaggac aatcccaaga cgaacggcat
     2161 ggaaaccgtg tccgatcttg ccgcggccta taatgccggc aagccggtgg tgctgggtac
     2221 gtcggcggaa ttccccaagc ggccggacgg cctgcctggg cttgagcaag tctatggttt
     2281 caaggcggga cgcgagaatg tcaggccgat ggatctggga ctcgcctaca aggcgctcgg
     2341 caatggcgat ctcgatgtga tcgcctcgca ggccaccgat ggccttatcg ccgcgatgaa
     2401 cctgaaagtg ctcaaggacg acaagggctt tttccccgac tacgccatga cgccggtggt
     2461 gcgcaaggaa gtgctcgacg ccaatcccga tctcaagggc atccttgaat cgctgtcggc
     2521 caagctcgat gacgcaacca tgcagcacct caatggcgag gtcgatgtcg acaagaagac
     2581 ggtggagcag gtcgctacgg acttcttgaa gcagaacaaa ctttaggaaa cagcagccgt
     2641 cactgcgccg tgcaagcggc gcgtaggcgg cggctgccag caacccgcct ggcatggcga
     2701 atgccgggcg gtagcctgat cggaaggaac cggaatgaac attttttcaa agcttggcgc
     2761 cgctgtgctg tcggcggccg tcgtcctgtc gggctgggcg gcgatggctg ccaacctgac
     2821 ggttggcggc aaggatttca ccgagcagct tgtgcttgcc gaaatgacca agcaattgct
     2881 ggaggcgaag ggctacaccg tcgacaagaa ggacggcatg ggcaccagca tcgtgcgtgc
     2941 ggcgctcgaa agcggcgagg tcgacatgta ttgggaatat accggcacct cgctggtcac
     3001 cttcaacaag atcaccgagc atctcagccc tgaacagacc tacgccaagg taaaggaact
     3061 tgatggcgcc aaggggctcg tttggctggc gccgtcgaaa gccaacaaca cctatgcgct
     3121 ggccatccgg cagaataatc cgaagaccga tggcatgaag acctattccg atcttgccgc
     3181 cgcctaccgc gatggcaagg aggtgctgat gggggcgacc gccgagttcg tcaagcggga
     3241 ggacggcctg cttggattgc agaagatgta tgatttcaag gcgggccgcg ccaatacgcg
     3301 accgctggat gtcggccttg tctataacgc gctcgccaat ggcgatgtcg acgtttcgac
     3361 cgtcggggca acggacgggc gcatcgccgc catgcacctg atcctgctga aggatgacaa
     3421 gagcttcttc ccgaattacg cgctcgcgcc ggtgatcgcc aaaaagacac tcgatgcgca
     3481 tccagacttg caaggcctgc tggaatcgct gtcgggcaag ttggatgacg ccacgatgca
     3541 gcggctgaac ggcgaggtgg atgtggagaa gaagaccgtt gaagacgtag cgaccggcta
     3601 tctcaagcaa gctgggctga tctagcaacg atggccggtt gcctgtcgca gccggcctgg
     3661 atagtgaaag tcgaagaaac cgccgatgac caccgactac gccagattga tcgacgccga
     3721 gacctgggcg ttcatcaacc gcatcaatgc cttttacccg cccgaaacgg cgaacctccc
     3781 catcgagcgt aaccgcgaga tctacgaccg gatgagccgt gcttttcata cggcctatcc
     3841 tgccggtgtc agcgcgcaag ccggcaccgt gccgctggct gaccacgacg ttgcgctgcg
     3901 catctaccga aaggccggtg gccccgaggc ggctgtggtg ctctatttcc acggtggcgg
     3961 ctttattctg ggcggtctgg acagccatga cgacatttgc gccgacatct gcgcgggcac
     4021 cggggccatt gtcatctcgg tggattaccg gctggccccg gaacaccccg gcgcggcagc
     4081 ttttgacgat gccgtggccg ccttcgaatg ggttggcgcc aactacccgc agccgattgt
     4141 tctcgtcggc gaatcggcgg gcggcaacct ggccgcatgc gtcgcccacc acactcgcgg
     4201 ccacgctctg gccgccatcg gccaggtgct gatctatcct ggcttaggcg gcgatgaagg
     4261 ccagggttcc tacgtaacgc acgccgaagc gccgatgctg tcgacgcgcg accttgattt
     4321 ttaccgcgcc gttcggaccg gtgaagcgaa cgtatcggac gatccgcgct atacgccgct
     4381 ggccgacaac gatttttccg agctgccgcc caccgtaatc ttcaccgcgc aatgcgaccc
     4441 gctttcatcc gatggtgaag cctatcgcga ccggttgttg cggggtggcg gcaaggcgcg
     4501 ctggcaggag gaggaggccc tgccgcacgg ctatttgcgc gcgcgtcaca ccgttggccg
     4561 ggcaagggcc agcttcgcgc gcattgtcga ggccatcggc atgctcggcg a
//
LOCUS       DASYCS010000056         5903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4505055,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000056 DASYCS010000000
VERSION     DASYCS010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 5903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5903
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4505055"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="VGM46_02115"
     CDS             complement(<1..891)
                     /locus_tag="VGM46_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018430527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1402 family protein"
                     /protein_id="HEY4191416.1"
                     /translation="MKKFLLGIVLVMLSAAAFPALAATPVPPGNRNVEQPDIPGASSR
                     RTQATNTTFQAKYRKVYALLQNDAKLRAKIKQAAAAYGIDPMHIVGAIVGEHTYNVDA
                     YDRLQTYYVKAISYLSSKLTFAYDGEDITTFIQRPEFAKCADQSGSYDLWECREQVWN
                     HTFRGKTVGGESFPNDRFGATFFQPYYAGQTFGLGQLNPLTALQMSDLVHKVSGLPRL
                     DVGDPNAVYKTIMDPDLTLPYVAATLRKSIDAYRSIAGFDISGNPGITATLYNVGNPE
                     QRAYALKAENQKRQAAGEPAK"
     gene            complement(1060..2373)
                     /gene="hslU"
                     /locus_tag="VGM46_02120"
     CDS             complement(1060..2373)
                     /gene="hslU"
                     /locus_tag="VGM46_02120"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015314577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEY4191417.1"
                     /translation="MSTFSPREIVSELDRFIIGQKDAKRAVAIALRNRWRRQQLEGQM
                     REEVMPKNILMIGPTGVGKTEISRRLAKLAGAPFVKVEATKFTEVGYVGRDVEQIIRD
                     LVEIAIGLVREKMREDVKARAHINAEERVLEALVGKTASPATRDSFRKKLREGELDDK
                     EIEVEVADTGNGGMPGFEIPGMPGANIGVMNINDMLSKAMGGKKTKMRKTTVKESYGL
                     LIEDESDKLLDQDEVVRRALEATQEDGIVFLDEIDKIANREGGMGAGVSREGVQRDLL
                     PLVEGTTVATKYGPVKTDHILFIASGAFHVAKPSDLLPELQGRLPIRVELRALEKDDF
                     VRILTETEASLIKQYIALMKTEGVELVFTDDAIDSLAGIAVDLNSTVENIGARRLQTV
                     MERVLDEISYDAPDRNGTAVTIDAAYVQKHVGDLSRNTDLSRFIL"
     gene            complement(2385..3554)
                     /locus_tag="VGM46_02125"
     CDS             complement(2385..3554)
                     /locus_tag="VGM46_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018689745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191418.1"
                     /translation="MPLAGGISAETLETAITEGTITPQQRDRLRALERIGPAGAESDD
                     ESGEENLRLIGGGNDLFVTVGTVLLTAGFFFVLNTLLPGQRLWIAALVALFSWAVAEI
                     VTRQHRMKLSSTVLSLFFVTAFVVALGSETLEHYAIQKPSSIWQLLALRQQVSSAGYL
                     FLGGVMAAVALYFWRFKVPILAGVFAIAATLLAFLQTALLLYDGVAAGTVAPPQLKDM
                     PELLRAALYMPLICGLIVFGTAVALDVFDRERRKIWSDCAFWLHVVSAPMLVHPLFIL
                     ATGQDVVMGRIEPDANATIMLAVLIIGFVYVALAIDRRSLLVPTLAYFGSLGIYYLVS
                     SASHTTGVPPFALILLVVGALVICFGAGWQRIRRLIIGSTLPTSVLDKLPPIKKV"
     gene            complement(3557..4153)
                     /locus_tag="VGM46_02130"
     CDS             complement(3557..4153)
                     /locus_tag="VGM46_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2585 domain-containing protein"
                     /protein_id="HEY4191419.1"
                     /translation="MSIPETRRGLSRDFPWQAGLVIAAVIIGIQAIALYVEGHPAICT
                     CGYVKLWHGVVQSSENSQHISDWYTPSHIIHGFLFYALSRFLFPRSSIWLRLAFALLI
                     EGGWEILENSPIIIDRYRTGTISLDYYGDSIINSVSDTLAMVLGFVMAKTLPVWVVVA
                     LAILFELGTGYMIRDNLTLNVIMLVHPMEAVKAWQGAV"
     gene            complement(4150..4698)
                     /locus_tag="VGM46_02135"
     CDS             complement(4150..4698)
                     /locus_tag="VGM46_02135"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006201295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY4191420.1"
                     /translation="MPPKQGKQRQAAPAYDFRAVTEADLPLLGRWLALPHMAEWWGDP
                     ATEIASIRDHIDSDGVEPLIVELDGRPIAYLQSYDPHMEDDHPYADQPFGTLGIDLSI
                     GVPELVGIGHGPAIIRQFTAELFEEGALRVVIDPAPGNARAIRAYEKAGFQPLGERKS
                     QYGHVLLMALDADQGADEGDDE"
     gene            complement(4688..5239)
                     /gene="hslV"
                     /locus_tag="VGM46_02140"
     CDS             complement(4688..5239)
                     /gene="hslV"
                     /locus_tag="VGM46_02140"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015314580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEY4191421.1"
                     /translation="MSNELSMHATTIVTVRKGGKVVIAGDGQVSLGQTIMKGNARKVR
                     RIGKGGNVIAGFAGATADAFTLLERLEAKLEQYPDQLTRACVELAKDWRTDRYLRRLE
                     AMMLVADKSVSLALTGNGDVLEPEDGVMAIGSGGNYALAAARALIDTDQDAEEIARKA
                     MKIASEICVYTNNNFVVEVIDAA"
     gene            5436..>5903
                     /gene="hisB"
                     /locus_tag="VGM46_02145"
     CDS             5436..>5903
                     /gene="hisB"
                     /locus_tag="VGM46_02145"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018430533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEY4191422.1"
                     /translation="MTARSAEVSRETKETEISVSLHVDGAGKSDIETGVGFFDHMLDQ
                     LSRHSLIDMTVRAKGDLHIDDHHTVEDTGIALGQALSKALGERRGIMRYASIDLAMDE
                     TLTRAAIDVSGRPFLVWNVAFSSPKIGTFDTELVREFFQALAQNAGITLHVTNH"
BASE COUNT         1089 a         1818 c         1849 g         1147 t
ORIGIN      
        1 ctttgccggt tcgccggcgg cctggcgctt ctggttttca gccttgagcg cataggcgcg
       61 ctgctccggg ttgccgacat tgtagagcgt ggcggtgatg cccggattac ctgaaatgtc
      121 gaagccggcg atgcttctgt acgcatcgat cgacttcctc agcgtcgccg ccacataggg
      181 cagcgtcaaa tccggatcca tgatggtctt gtagacggcg ttggggtcgc ccacgtcgag
      241 cctgggcagg cccgaaacct tgtgcaccag atcgctcatc tgcaaggcgg tcagcgggtt
      301 cagctggcca agcccgaagg tctggccggc ataatagggc tggaagaagg tggcgccgaa
      361 gcggtcgttg ggaaagcttt cgccccccac cgtcttgccg cgaaaggtat ggttccacac
      421 ctgctcgcgg cattcccaca aatcgtagct gcccgactgg tccgcgcatt tcgcaaattc
      481 gggacgctgg atgaaggtgg tgatgtcctc gccatcatag gcgaaggtca gcttgctcga
      541 caggtaggag atcgccttga cgtagtaggt ttgcaggcgg tcgtaggcgt cgacattgta
      601 ggtatgctcg ccgacgatgg cgccgacgat gtgcatcggg tcgatgccat aggccgccgc
      661 cgcctgcttg atcttggcgc gcagcttggc gtcattttgc agcagcgcgt agaccttgcg
      721 gtatttggcc tggaaggtgg tgttggtggc ctgcgtgcgc cggcttgaag cgcccggaat
      781 atcgggctgt tccacgttgc ggtttcccgg cggcacgggc gtcgcggcca gcgccggaaa
      841 cgctgctgcc gaaagcatca cgaggacaat gccgagaagg aattttttca tgcgccgccg
      901 cgaccttgcc catagcgttc gggttatccg gggcgcaacc tagaaaaacc gccgtggcaa
      961 gtcgagcctt cagtcaaggc aagcgactaa aggtcaacat atgcttccat ttggccacac
     1021 atcttgcgat gtgcggccaa atttgccgtc cgccgtgcct tagaggatga agcgcgacag
     1081 atccgtgttg cgcgacaggt cgccgacatg cttctgcaca taggcggcgt cgatggtgac
     1141 ggcggtgccg ttgcggtcgg gcgcgtcgta ggaaatctcg tccagcaccc gttccatcac
     1201 cgtctgcaag cgccgcgcgc cgatattctc gacggtggag ttgaggtcca ccgcgatgcc
     1261 ggccagcgaa tcgatggcgt cgtcggtgaa gacgagctcg acgccctccg tcttcatcag
     1321 cgcaatatac tgcttgatga ggctggcttc ggtttcagtg aggatgcgga cgaaatcatc
     1381 cttttccagc gcgcgcagtt cgacgcggat cggcaggcgg ccctgcaact ccggcagcag
     1441 gtcggacggc ttggcgacat ggaacgcgcc cgaggcgatg aacaggatat ggtccgtctt
     1501 caccggcccg tatttggtcg caaccgtggt gccctcgacc agcggcagca ggtcgcgctg
     1561 cacgccttcg cgcgagacgc cggcgcccat gccgccctcg cggttggcga tcttgtcgat
     1621 ctcgtcgagg aagacgatgc cgtcctcctg cgtcgcctcc agcgcgcggc gcaccacctc
     1681 gtcctggtcg agcagcttgt cggactcgtc ctcgatcagc aggccgtagg attccttgac
     1741 cgtcgtcttg cgcatcttgg tcttcttgcc gcccatcgcc ttggacagca tgtcgttgat
     1801 gttcatgacg ccgatattgg cgcccggcat gccgggaatc tcgaagccgg gcatgccgcc
     1861 attgccggta tcggccactt cgacctcgat ttccttgtcg tccagctcgc cttcgcgcag
     1921 cttcttgcgg aagctgtcgc gggtcgccgg tgacgcggtc ttgccgacca gcgcttccag
     1981 cacgcgctcc tcggcattga tgtgggcgcg cgccttgacg tcctcgcgca ttttttcacg
     2041 cacgaggccg atggcgatct cgacgagatc gcggatgatc tgctcgacat cgcggccgac
     2101 atagccgact tcggtgaact tggtcgcctc gaccttgacg aagggcgcgc ccgccagctt
     2161 ggccagccgg cgcgatatct cggtcttgcc gacgccggtc ggcccgatca tcaggatgtt
     2221 cttcggcatc acctcttcac gcatctggcc ttccagctgc tggcggcgcc agcggtttcg
     2281 caaggcgatg gccacggcgc gcttggcgtc cttctggccg atgatgaagc ggtcgagttc
     2341 cgaaacgatt tcgcgggggg aaaatgtgct catgaattac agatctacac ttttttgatg
     2401 ggaggtaatt tgtcgagcac ggatgtgggg agcgtggagc cgatgatcaa gcgccttatt
     2461 ctctgccagc ccgcgccgaa gcagatgacg agcgcgccga ccaccagcag gatcagcgca
     2521 aacggaggaa cgccggtggt gtgggaggcg gaactgacca ggtaataaat gccgagcgag
     2581 ccgaaatagg caagcgtcgg caccagcagc gaccgcctgt cgatggccag cgccacatag
     2641 acgaagccga tgataaggac ggccagcatg atggtggcgt tggcgtcggg ttcgatccgg
     2701 cccatgacga cgtcctgccc ggtagccagg atgaacagcg gatgcaccag catcggcgcc
     2761 gacacgacat gcagccagaa tgcgcagtcc gaccagattt tgcgccgctc ccggtcgaaa
     2821 acatcgagcg ccaccgccgt gccgaagacg atgaggccgc agatcaacgg catatagagc
     2881 gccgcgcgca acaattcggg catgtccttg agctgcggcg gggcgaccgt gccggcggct
     2941 acgccgtcat aaagcagcag ggccgtctgc aagaacgcca gaagcgtcgc ggctatggcg
     3001 aagacgcccg ccagaatggg aaccttgaag cgccagaaat aaagcgccac cgccgccatg
     3061 acgccgccga ggaagagata gccggcgctc gaaacctgct gtcgcaacgc cagcaattgc
     3121 cagatcgaag agggtttctg gatcgcataa tgctcaaggg tttccgaacc cagcgccacg
     3181 acgaaggccg tcacgaagaa aaggctcaac accgtcgagg acagcttcat gcggtgctgg
     3241 cgcgtgacga tttcagcgac cgcccatgaa aacagcgcca ccagcgccgc gatccacagg
     3301 cgctggccgg gaagcagcgt attgagaacg aaaaagaagc cggccgtcag caagaccgtg
     3361 ccgacggtga cgaaaagatc gttgccgccg ccgatcaggc gcaaattctc ctcaccgctt
     3421 tcgtcgtcgc tttccgcgcc tgccggcccg atgcgctcca gcgctcgcag ccggtcgcgc
     3481 tgttgtggcg tgatcgtccc ttcggtaatc gcggtttcga gcgtttccgc gctgatgcct
     3541 cccgctaacg gcatcgctag accgctccct gccaggcttt taccgcttcc atcggatgca
     3601 ccagcatgat gacgttgagc gtcagattat cccggatcat gtagccggtg ccgagttcga
     3661 aaaggatggc cagcgccacc accacccaga caggcagggt tttcgccatc acgaagccca
     3721 gcaccatggc cagcgtgtcg gacaccgaat tgatgatgct gtcgccgtaa tagtcgagcg
     3781 agatggtgcc ggtgcggtag cggtcgatga tgatggggct gttttcaagg atttcccagc
     3841 cgccttcgat cagcagtgca aacgccagcc tcagccagat cgaggagcgc ggaaacagga
     3901 agcgcgacag cgcatagaac aggaagccgt ggatgatgtg cgatggcgtg taccagtcgg
     3961 aaatgtgctg ggagttctcc gagctttgaa cgacgccgtg ccacagcttg acgtaaccgc
     4021 aggtgcagat cgccgggtgg ccctcaacat agagcgcgat ggcttggatg cctatgatga
     4081 cagcggcgat gaccagcccg gcctgccacg gaaaatcccg cgacaggccg cgcctcgttt
     4141 caggaatgct cattcgtcgt ccccctcgtc ggccccttgg tcggcatcga gcgccatcaa
     4201 aagcacatgg ccgtattgcg actttcgttc ccccaaaggc tggaagccag ccttttcgta
     4261 agcccttatc gcccgtgcgt tgccgggagc gggatcgatg acgacgcgca gcgcgccttc
     4321 ctcgaacaat tccgccgtga attgcctgat aattgccggg ccgtggccga tgcctaccag
     4381 ttccggcacg ccgatggata ggtctatgcc gagcgtcccg aaaggctggt ccgcataggg
     4441 atggtcgtct tccatatgcg gatcgtagct ttgcagatag gcgatgggcc gtccgtccag
     4501 ctcgacgatc agcggctcga cgccgtcgct gtcgatgtga tcgcggattg aggcgatttc
     4561 cgttgccgga tcgccccacc attcggccat gtgaggcagc gccagccaac ggccaagcaa
     4621 gggcaggtca gcctcggtga ccgcgcggaa atcataggcc ggcgcggcct gccgctgctt
     4681 gccctgctta ggcggcatcg atcacttcga cgacgaagtt gttgttggtg tagacgcaga
     4741 tttcggaggc gatcttcatc gccttgcgcg ctatttcctc ggcgtcctgg tcggtgtcga
     4801 tcagcgcgcg cgccgccgcc agcgcatagt tgccgccgga gccgatggcc attacgccgt
     4861 cttccggctc cagcacgtcg ccattgccgg tcagcgccag cgacacggac ttgtcggcca
     4921 ccagcatcat ggcttccagc cggcgcaaat agcggtcggt gcgccagtcc ttggccagtt
     4981 ccacgcaggc gcgggtcaac tggtctggat attgctccag cttggcttcc agccgctcca
     5041 gaagcgtgaa ggcgtcggcc gtcgcgccgg caaagcctgc aatgacgttg ccgcccttgc
     5101 cgatgcggcg aaccttcctg gcattgccct tcatgatggt ctggccaagg ctcacctggc
     5161 cgtcgccggc aatgaccacc ttgccgccct tgcgcaccgt cacgatggtc gtggcgtgca
     5221 tgctcagttc atttgacatg ttttctgctc cgatgccgcg ctatcccggt aaggcgatgt
     5281 ggcgcgttac gagtgatttg atcggtcata tgtatgcaag ccaggggcga ttgcaaaccg
     5341 gtgagacagg cggttttgcc aactggcatt cgcgcggtca tgctggtaga aggcactcgc
     5401 tttaacgctt gggtttccag cacagggatg aggccatgac tgcccgatcc gccgaagtga
     5461 gccgcgagac gaaggaaacc gagatttcgg tttcgcttca tgttgatggc gccggcaagt
     5521 ccgacatcga aaccggtgtc ggcttcttcg accatatgct cgaccagctg tcgcgccatt
     5581 cgctgatcga catgacggtg cgcgccaagg gcgacctgca tatagacgac caccacacgg
     5641 tggaagacac aggcatcgcg cttggccagg ccctgtccaa ggcgctgggc gagcggcgcg
     5701 gcatcatgcg ctatgcttcg atcgaccttg ccatggacga gacgctgacg cgcgccgcca
     5761 tcgatgtttc gggccggccg ttcctggtat ggaacgtggc gttctcctcg cccaagatcg
     5821 gcacgttcga caccgagctg gtgcgtgagt tcttccaggc gctggcgcag aatgccggca
     5881 tcaccctgca tgttaccaac cat
//
LOCUS       DASYCS010000057         5436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1026352,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000057 DASYCS010000000
VERSION     DASYCS010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 5436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5436
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026352"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VGM46_02150"
     CDS             <1..351
                     /locus_tag="VGM46_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191423.1"
                     /translation="ISGLMSDTERQRAERDLEVDPAFRDAVMQLAERMHLFDGPSSPE
                     DALDDSWKLVAARIAELPQMQPMAIEANSTTRPAGNERADRFALFSTKPARALMIGIG
                     VILAFALCYFTTKS"
     gene            complement(335..832)
                     /locus_tag="VGM46_02155"
     CDS             complement(335..832)
                     /locus_tag="VGM46_02155"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY4191424.1"
                     /translation="MPATIEPMREEDVEAVARLRVESFFKDSGRTHAEDVADLRDMLR
                     GDGFECSMVARIGAEPVGSVLFVRHELEPAHALTPWLAGLAVAKAFRGRGIGAALVRA
                     VEAHARAVGAEPLYLYTWQARDFYAALGWQAVESLVQDGEPMFLMMRRLAPAAQDLPA
                     FRIWS"
     gene            complement(915..1904)
                     /locus_tag="VGM46_02160"
     CDS             complement(915..1904)
                     /locus_tag="VGM46_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014766642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY4191425.1"
                     /translation="MKRIVLGLLAATAMVLPAFAADVQPAILYDLGGKFDKSFNEAAY
                     NGAEKFKAETGTPYVEFEVSNASQREQALRRFAEDGRNPIVMAGFAWVDPLTKVAAEY
                     PELNFVVIDDQVDLPNVRSLKFKDYEGSYIVGVLAGMASKTKKVGFVGGMDVPLIRKF
                     ECGYVGGAKAAGATEVIQNMTGDTPAAWNDPAKGSEIAKTQIDQGADVVYAAAGGTGV
                     GVLQAAADAGKLGIGVDSNQNGLQPGKVLTSMLKRVDVAVYTAFMDGKNGTFKGGLSN
                     LGLKEGGVDYAMDDNNKDLVTPEMKAAADKAKADIIAGTIEVHDYSTDNKCPY"
     gene            complement(2133..2786)
                     /gene="msrA"
                     /locus_tag="VGM46_02165"
     CDS             complement(2133..2786)
                     /gene="msrA"
                     /locus_tag="VGM46_02165"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEY4191426.1"
                     /translation="MFFLSDMLNKKLNLPKPGEALPGRAKPIRTATRHFVSRLALKEP
                     YPDATETAVFAMGNFHDAERLFWKTEGVWVTAAGFCGGATANPTYQEVSTGLTGHTQA
                     VLVVFDPAIVSYAALLKLFWQSHDPTQGMRQGNDIGTFYRSMIHTRGEAQHGAALASR
                     DAFQVALLGIGLGRITTQIAPGPNFFFAEAEHQQYLAKNPDGAGGLRGTGAVLEIAS"
     gene            complement(2809..3132)
                     /locus_tag="VGM46_02170"
     CDS             complement(2809..3132)
                     /locus_tag="VGM46_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191427.1"
                     /translation="MFRFVFRLAAMVALSISVIMAVLDTTRTVAASALVLTPLKTSWL
                     AVSPDTLTGFEAFTREKAGALLWDGVVLRVLDLPGFAVFAALALLLYAIGYKRERRGG
                     FAAVN"
     gene            3467..3556
                     /locus_tag="VGM46_02175"
     tRNA            3467..3556
                     /locus_tag="VGM46_02175"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3501..3503,aa:Ser,seq:gga)
     gene            complement(3686..4336)
                     /locus_tag="VGM46_02180"
     CDS             complement(3686..4336)
                     /locus_tag="VGM46_02180"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY4191428.1"
                     /translation="MKFGAGIALCALAVCGGCVAGPNPSQISWEWFNVAETYKAYPRR
                     TEADLRISAHYYALAANKGNAAAAYKLAEMYENGAGVAKDDDAALKWYRAAAVAGDKY
                     GQFRVGWFYQHGLSTPKDMQQARLWYERSAKQDNEWAYHMLAFMLADGDGVGQDTALA
                     RTYFERSLPRTNDNWAKWKLAMLVEDSDPRRARQLLAESAAAGNVQAGEELRRKDW"
     gene            4540..4851
                     /locus_tag="VGM46_02185"
     CDS             4540..4851
                     /locus_tag="VGM46_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006204433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191429.1"
                     /translation="MSRHLKITIGFLAGLALLPALGADASAIEIITPTDSNALLRQGR
                     GNELRALRNQIQRQQFQQQQQQNRTQDRRVAPPPVLDVPKMKPTCQKQVYGNGYLKSC
                     R"
     gene            4964..5434
                     /locus_tag="VGM46_02190"
     CDS             4964..5434
                     /locus_tag="VGM46_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018429802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY4191430.1"
                     /translation="MSAEKKNAIRKAKKGETIRQVAAVPYRINAQGGLEVMLVTSRET
                     RRFIVPKGWPMKGKSGRKAAEIEAAQEAGVTGTVIREPSGAYHYWKRLSNRFVRVNVV
                     VYLLEVKEELATWQEREARQRSWLSPAEAALLIDEPELSTLIRTLAPPVRPQPD"
BASE COUNT         1096 a         1758 c         1575 g         1007 t
ORIGIN      
        1 atatcgggct tgatgagcga caccgagcgc caacgggccg aacgcgacct tgaggtcgat
       61 ccggcctttc gcgatgcggt gatgcaactg gccgagcgga tgcacctctt cgatggccca
      121 tcctcgcccg aggacgcttt ggacgatagc tggaagctgg ttgccgcccg catcgcggaa
      181 ctgccgcaga tgcaacccat ggcgatcgag gccaacagca ctacccgccc ggcaggcaac
      241 gagcgggccg accgtttcgc gctattttcc acgaagccgg cacgcgccct gatgatagga
      301 atcggcgtga tactggcttt cgcactgtgc tacttcacga ccaaatccta aaggcaggca
      361 ggtcctgagc ggcgggcgcg agccggcgca tcatcaaaaa catcggttcg ccgtcctgga
      421 cgaggctctc gaccgcctgc catccgagcg cggcgtaaaa atctcgcgcc tgccacgtat
      481 agaggtaaag cggttccgcg ccgaccgcgc gggcatgcgc ctcgaccgca cgtaccagcg
      541 cggcgccaat tccccgcccc ctgaaagctt tcgcaacagc cagacccgcg agccagggcg
      601 ttaatgcgtg agccggctcc agttcgtgac gaacgaacaa aaccgatccg accggttccg
      661 caccgatccg cgcaaccatg gagcattcga acccgtcacc gcgcagcatg tcgcgcaggt
      721 cggccacatc ctcggcatgc gttctgccgc tatccttgaa aaaactttcg acgcgaagcc
      781 gtgcaacagc ctcgacgtct tcttcccgca taggctcgat cgtggctggc atgaaccatc
      841 ccaaaaaaaa ccgccgggac atgcccggcg gttcgcaacg gctttatgcc gcaatcaata
      901 tggctgggct ttcttcaata ggggcatttg ttgtcggtgc tgtagtcatg cacctcgatg
      961 gtgccggcga tgatgtcggc cttcgccttg tcggcggcgg ccttcatttc tggcgtgaca
     1021 agatccttgt tattgtcgtc catcgcatag tcgacgccgc cttccttgag accgagattg
     1081 ctcaagccgc ctttgaacgt gccgttcttg ccatccatga aggcggtgta gacggcgaca
     1141 tcgacacgct tgagcatcga ggtcagcacc ttgcccggct gaaggccgtt ctggttggaa
     1201 tcgacgccga tgccgagctt gcccgcatcg gccgccgcct gcaacacgcc gacgccggtg
     1261 ccgccggccg ccgcataaac cacgtccgcg ccctggtcga tctgcgtctt ggcgatctcg
     1321 ctgcccttgg ccggatcgtt ccacgccgcc ggcgtatcgc cggtcatgtt ctggataact
     1381 tccgtcgcgc ccgccgcctt ggcgccgccg acatagccgc attcgaattt gcggatcagc
     1441 ggaacgtcca tgccgccgac gaagccgact ttcttggttt tcgaagccat gccggccaga
     1501 acgccgacaa tataggagcc ttcgtaatcc ttgaacttga gcgagcgcac attgggcagg
     1561 tcgacctggt cgtcgatgac cacgaagttc agttcgggat attcggcggc aaccttggtc
     1621 agcggatcga cccaggcaaa acccgccatg acgatcggat tgcggccatc ttcggcaaag
     1681 cggcgcagcg cctgctcgcg ttgcgaggcg ttcgacactt cgaattcgac gtaaggcgtg
     1741 ccggtttcag ccttgaattt ttcagccccg ttataggctg cctcattgaa cgatttgtcg
     1801 aacttgccgc ccagatcgta aaggatcgcc ggctgaacat cggcggcgaa ggccggcaga
     1861 accatggcgg tcgcggccag aaggccaagc acaatacgtt tcatgtcatc cacaccctgt
     1921 cggtttcttt tctaggtcgg cggctccagc tcggcggcgt cgcctgccgg ccttctaaaa
     1981 ggccgcggta acgtccggcc gagcattttg gccctgccgc caggcaatct tgcacggcgc
     2041 gaaacaaaat tcatcaggaa tcttggtttg ctaaaatacg cgcccggcat gggttaagcc
     2101 cggtcgttgc cggcgtttgc gaaccggcta aatcagctcg cgatttccag aacggcgccg
     2161 gttcccctca ggccgccggc gccatccggg ttcttcgcca gatattgctg gtgttccgct
     2221 tcggcgaaaa agaagttcgg ccccggcgcg atctgcgtcg tgatgcgccc aagcccgatc
     2281 ccgagcagcg ccacctggaa cgcatcgcgc gaagcaagcg ccgcgccatg ttgcgcctcg
     2341 ccgcgcgtat ggatcatcga gcggtagaag gtgccgatgt cgtttccctg ccgcatgccc
     2401 tgcgtgggat cgtggctttg ccaaaacagc ttcagcaacg ccgcatagga gacaatcgcc
     2461 gggtcgaaga caacgagcac cgcctgtgta tggccggtaa ggccggtgct cacttcctga
     2521 taggtcgggt tggccgtcgc gccgccacaa aagccggccg ccgtcaccca tacgccctcg
     2581 gtcttccaga acaggcgctc ggcatcgtgg aaattgccca tcgcaaacac cgccgtttcg
     2641 gtggcgtcgg gatacggctc tttcaacgca agcctggaga cgaaatgccg ggtggccgtg
     2701 cgaatgggct tcgcccggcc cggcaacgcc tcgccgggct tgggaaggtt gagcttcttg
     2761 ttcagcatgt cgctgagaaa gaacatgggg cctccccgtt accggatgtc agttcacggc
     2821 cgcgaagcca ccgcgccgct cccgtttata cccaatcgca taaagaagca gggcaagcgc
     2881 ggcgaacacc gcaaaaccgg gaagatcgag cacccgcaaa acgaccccgt cccacagcag
     2941 cgcgcccgcc ttctcgcgcg taaaggcctc aaagccggta agcgtatcgg gcgagacggc
     3001 aagccagctt gtcttgagcg gggtgagcac cagcgccgaa gccgcgaccg tgcgcgtcgt
     3061 gtccagcacc gccatgatga cggagatcga cagcgccacc atggcggcaa gacgaaaaac
     3121 gaagcgaaac atgcagactt tccaagcgat cgttcaaccg aaacgatagg acgagcttgc
     3181 ccgcgccgca atcgtttcgc gccgattgac gccgccggcg ccgcccgaaa gcggcaattc
     3241 cgacgcaacg ccgatatctt gggcgaaagc aacgccatta tggcttggca ttcggcttag
     3301 gatcgactag atcagccccg caccggatgc tttgacggct ctggtgcgag ggaaacggcg
     3361 ttgcaggttg gcagcgcccg cagcacggat accggctgta ttcccaaggg atttgcgccc
     3421 atccccaacc cgcccggcat aaccagccga cgcgctgcgc aaagacggag aggtggccga
     3481 gtggttgaag gcgcacgcct ggaaagtgtg tttacgggag accgtaacgc gggttcgaat
     3541 cccgctctct ccgccatatt tcctgactaa tccattcttt tcatatcgcg caatcaaagc
     3601 cgagtgcatg gaatttcaat cgttttcggt aggaaatgcc ggaaataggt agcgtaaggc
     3661 gctatccatt cgggaaaacc ggcaattacc aatccttccg ccgcagttcc tcgcccgcct
     3721 gaacattgcc cgcggcggcg gattcggcca gcaattgccg cgcgcgtcgc gggtcgctgt
     3781 cttcgacaag catggcgagt ttccatttcg cccaattgtc gttggtgcgc ggcagcgaac
     3841 gctcgaaata tgtgcgcgcc agcgcggtgt cctgtcccac gccgtcgcca tcggccaaca
     3901 tgaaggccag catgtgatag gcccattcat tgtcctgctt ggccgagcgc tcgtaccaga
     3961 ggcgagcctg ctgcatatcc ttcggcgtcg acagcccatg ctgataaaac cagccgacgc
     4021 gaaattgccc gtatttgtcg ccggccacgg ctgccgcgcg ataccatttc agcgccgcgt
     4081 cgtcgtcctt ggcgacgcct gcgccgttct cgtacatctc cgccagcttg taggcggccg
     4141 ctgcattgcc cttgtttgcg gccagcgcat agtaatgcgc cgaaatacgc aaatccgcct
     4201 cggtgcgcct gggataggcc ttgtacgtct cagccacatt gaaccattcc cagctgatct
     4261 gcgaaggatt tggcccggcc acgcagccgc cgcacaccgc cagcgcgcaa agcgcgatgc
     4321 ctgccccgaa cttcaattcc acccgccccc ggctcgatcc gacaagctat acccgaccgg
     4381 gcaatttgca attgcaagcc gagatggttt ggcgtctacc tcgccggagg ggaatgcggc
     4441 agcgaatatt ttccccaggc aggcaatcgc gcacgcttta tcggtggcgt acctcgtaaa
     4501 tcgccctatg ttttacccaa acggagcctg taacggccaa tgtcgcgcca tttgaaaatc
     4561 acgatcggtt tccttgccgg gctggccctg ttgccggctt taggcgcgga cgcctccgcc
     4621 atagagatca tcaccccgac tgacagcaat gcgctattgc ggcaggggcg cggcaacgaa
     4681 ttgcgcgcgc tgcgcaacca gatccaacgc cagcagttcc agcagcagca acaacaaaac
     4741 cgcacacagg acagacgagt agcaccgccg cccgttctcg acgttcccaa aatgaaaccg
     4801 acatgccaga agcaggttta tggcaatggc tatctgaaaa gctgccgctg aagaaccttc
     4861 accgcccgcc ggccgccaat tccacccgct tggccttccg cagcaactgc cggaataatt
     4921 tttccaacat cgccagaaag cttcgataaa tccgtgatta cagatgtccg ctgaaaagaa
     4981 gaacgcaatc cgcaaggcaa agaagggcga aaccattcgt caggtcgccg ctgtgcccta
     5041 tcggatcaac gcccagggcg ggcttgaggt catgctggtc acgtcgcgcg aaaccaggcg
     5101 cttcatcgtt cccaagggct ggccgatgaa aggcaagagc ggccgcaagg cagccgagat
     5161 cgaggccgcc caggaggccg gcgtcaccgg gacggtcatc agggaaccga gcggcgctta
     5221 tcactactgg aaacggcttt cgaaccgctt cgttcgcgtc aatgtggtgg tctatctgct
     5281 tgaggttaag gaggaactgg ccacctggca ggagcgggaa gcgcgccaac gctcgtggct
     5341 ctcgccggcg gaagccgcct tgctgatcga cgagcctgaa ctgtcgacgc tgatccgcac
     5401 gctggcgccg ccagttcggc cgcagcccga ttaagc
//
LOCUS       DASYCS010000058         3191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_737630,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000058 DASYCS010000000
VERSION     DASYCS010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737630"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..2068)
                     /gene="iolC"
                     /locus_tag="VGM46_02195"
     CDS             complement(113..2068)
                     /gene="iolC"
                     /locus_tag="VGM46_02195"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HEY4191431.1"
                     /translation="MSDVAEARRAPLDVIAIGRASVDLYGQQIGSRLEDITSFAKSVG
                     GCPANIAVGTARLGLRSALLTRVGNEQMGRFIGEQLLREGVSVEGIRTDPDRLTALVL
                     LSVEDEGVSPMIFYRADCADMALAEEDIDEAFIASARAIVVTGTHFSRDNSDAAQRKA
                     IRAIKAAGGKVVFDIDYRPNLWGLAGHAEGFGRYVKSERVTARLKTVLPDCDLIVGTE
                     EEIMIASGADDCLGALKAIRALSAATIVLKRGAMGCVVYEGEIGDELEDGVVGQGFPI
                     EIYNVLGAGDAFMSGFLRGWLGGESLATAATWANACGAFAVSRLLCAPEYPTFEELQF
                     FLDHGSKHHALRKDAEINHIHWATNRRRDLPSLMVFACDHRAQLEAVAEREGADFSRI
                     DAFKLLAVKAAAKVAAGREGYGMLLDEKYGRQAMFEFGRHSFAWLGRPVEQPGSRPLR
                     FEFSQDIGSQLVDWPVGHCIKCLCFYHPDDPTELKVEQQQKLRALFEASRKVGRELLI
                     EIIAGKHGALDDDTVARALEELYALGIRPDWWKLEPQASSAAWAKIEAVVTQNDPWCR
                     GVLLLGLEAPPDELESAFAAAAKAPIVKGFAVGRTIFIHAAEKWLAGEMSDDQAIADM
                     AARFERLAGAWLAARHHGGMQPREERP"
     gene            complement(2193..3047)
                     /locus_tag="VGM46_02200"
     CDS             complement(2193..3047)
                     /locus_tag="VGM46_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006325703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEY4191432.1"
                     /translation="MNERAPRDFETLRATILERRHALPKRIAQIAAYALDNPDDIAFG
                     TAASIAGSAGVQPSTLIRFAQQFGFDGFTSLQSVFRERLRERNSSYDERLAALRTKAG
                     PRHRSIFDGFVAAAIGSLDAVSHAIDEAQLEQAIAILAKAETIHILARRRSYPVACYI
                     AYALAKLEIRNQLIESAAGLDAEMLDFAKPADAAIVISFAPYAPATIAQARALSERGV
                     PVVAITDSAFSPLAQFSKVWFEVAEADFAGFRSLSATMALAMALSVGAAEKRRSAGTR
                     KPPRIRVG"
BASE COUNT          593 a         1048 c          979 g          571 t
ORIGIN      
        1 tggccgaaga tcgcccagac gccgccgaag accggctgct tcacgccgtc tatctcggtc
       61 atctggcgcg acaggaaacg ggtgagcgcc tgcgccatcg tcagccggat tgtcatggcc
      121 gttcctcccg tggctgcatg ccgccgtgat gccgcgcggc aagccatgcc ccggccagcc
      181 gttcgaagcg cgccgccatg tcggcgatcg cctggtcgtc cgacatttcg ccggccagcc
      241 atttttcggc ggcatgaatg aaaatcgttc gcccgaccgc aaaacccttg acgatgggag
      301 ccttggcggc ggcggcgaag gctgactcaa gttcgtccgg cggcgcttcc agcccaagca
      361 gcaacacacc ccggcaccat gggtcgtttt gcgtgacgac cgcttctatc tttgcccagg
      421 cggcgcttga ggcttgcggc tccagcttcc accagtcggg cctgatgccc agcgcgtaaa
      481 gctcctcgag cgcgcgcgcc accgtgtcgt cgtccagcgc gccatgtttt ccggcaatga
      541 tctcgatcag caattcgcgg ccgacctttc gcgacgcctc gaacagcgcg cgcagctttt
      601 gctgctgctc gaccttcaat tcggtcgggt cgtccggatg gtagaagcac aggcatttga
      661 tgcagtggcc aaccggccag tcgaccagtt gcgagccgat gtcctgcgaa aactcgaagc
      721 gcagcgggcg cgagccgggc tgctcgaccg gacggccgag ccacgcaaac gaatggcgcc
      781 caaattcgaa catcgcctgg cggccatatt tctcgtccag cagcatgcca tagccttcgc
      841 gccccgccgc gaccttggcc gccgccttga cggccaacag cttgaaggcg tcaatgcgcg
      901 agaaatcggc gccctctctc tccgccaccg cctcaagctg ggcgcgatga tcgcaggcga
      961 ataccatcag cgagggaaga tcgcgccggc ggttcgtcgc ccaatggatg tggttgatct
     1021 ccgcatcctt gcgcaaggcg tggtgcttgc tgccatgatc gaggaagaat tgcagttcct
     1081 cgaatgtcgg atattcgggc gcgcagagca ggcgcgacac cgcgaacgcg ccgcaggcat
     1141 tggcccatgt cgccgccgtc gccaggcttt cgccgcccag ccagccgcgc aggaagccgg
     1201 acatgaaggc gtcgcccgcg ccaagcacat tgtagatttc gatgggaaaa ccctgcccga
     1261 cgacgccatc ctcaagctcg tcgccgatct cgccttcata gacgacgcag cccatggcgc
     1321 cgcgcttcaa cacgatggtt gccgccgaca gcgcgcggat ggccttcagc gcgcccaggc
     1381 aatcgtcggc gcctgacgcg atcatgatct cttcctcggt gccgacgata aggtcgcagt
     1441 cgggcagcac cgtcttcaac cgggcggtca cccgctcgga tttgacgtag cgcccaaacc
     1501 cttcggcatg gccggccagc ccccacagat taggccggta gtcgatgtcg aacaccacct
     1561 tgccgccggc cgccttgatg gctcggatcg ccttgcgttg ggcggcatcg ctgttgtcgc
     1621 gcgaaaaatg cgtgccggtg acgacgatgg cgcgcgccga ggcgatgaaa gcctcgtcga
     1681 tgtcttcttc ggccagcgcc atgtcggcac agtcggcgcg gtagaagatc atcggcgaaa
     1741 ctccctcatc ctcgaccgat aaaagcacca gcgccgtcag ccgatccgga tcggttcgaa
     1801 tcccctcgac cgagacgccc tcgcgcagca attgttcgcc gatgaagcgg cccatctgct
     1861 cgttgccgac acgggtgagc agcgccgaac gcaagccaag ccgcgccgtg ccaaccgcaa
     1921 tattggccgg gcagccgccg accgacttgg cgaaagaggt aatgtcttcc agcctggagc
     1981 cgatctgctg gccgtagaga tcgaccgagg cgcggccaat ggcgatgaca tcgagcggcg
     2041 cgcggcgggc ttcggcgaca tcgctcatca taccctcccg gcagaatttc atcaggcgat
     2101 cgcgcggaaa ggcgcaacct ttatatgaaa taataattcc aactggtagt caatatggaa
     2161 tgcgtgttcc ctaccgttgc ctgcgtccga agtcagccga cgcgtatcct cggcggcttc
     2221 ctggttccgg cgctgcgccg cttttcggcc gcgccgacgc ttagggccat ggccaacgcc
     2281 atcgtcgccg atagcgaacg gaagccggca aaatcggcct cggcgacctc gaaccagacc
     2341 ttggaaaact gggcaagcgg cgaaaaggcc gagtcggtga tcgcgaccac cggcacgccg
     2401 cgttccgaca gcgcgcgcgc ctgcgcgatg gtggccggcg catagggcgc aaagctgatg
     2461 acgatggcgg catcggccgg cttggcgaaa tccagcattt ccgcatcgag accggccgcc
     2521 gattcgatca actggttgcg gatctccagc ttggccagcg cataggcgat gtagcaggcg
     2581 actggatagg accgccgcct cgccaatata tggatcgttt cggccttcgc cagaatagcg
     2641 attgcctgtt ccagctgcgc ttcatcgatg gcgtgcgaca ccgcatccag cgaaccgatg
     2701 gcggcggcga cgaaaccgtc gaagatcgag cggtgccgtg ggccggcctt ggtcctgagc
     2761 gccgcaagcc gttcgtcata ggaagaattg cgttcgcgca ggcgctcgcg aaacaccgat
     2821 tgcaggctgg tgaagccgtc aaagccgaat tgctgggcaa aacggatcag cgtcgaaggc
     2881 tggacgccgg ccgacccggc gatgctggcg gcggtgccga aggcaatatc gtcgggattg
     2941 tccagcgcat aggcggcaat ctgcgcgatg cgcttgggca aggcgtgacg ccgctccagg
     3001 atcgtcgcac gaagggtctc gaagtctcgt ggcgcccgtt cgttcatgtc cgcctcaaca
     3061 gatttcacgt tttcccagag cggttgtggt ttttacccaa tcgacaaaac cgctctatct
     3121 ctttgttttg gccgcatttc cgggcgcaaa accgtttcac acttttgctg gaatgctcta
     3181 gtgccgagga a
//
LOCUS       DASYCS010000059         4680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3781905,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000059 DASYCS010000000
VERSION     DASYCS010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4680
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3781905"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /locus_tag="VGM46_02205"
     CDS             complement(<1..989)
                     /locus_tag="VGM46_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY4191433.1"
                     /translation="MPTAQPKLNAFPIFLKVEGRDVIIVGGGDEALAKARLVGQSSAR
                     LHIVAEQVSEDFHQWIVANEAIHIEAAYLASHLEGAAMVFAASGNGELDRMVSRDARA
                     LGIPVNAVDRPELCDFFTPALVNRAPVTVAIGTEGAGPVLAQMIRARIDQMLSPSLGP
                     LASLAASFRDAAEALLPKGNVRRRFWREFFTGAPARALAAGQPAKANGAAADLLFSTT
                     PAAGHVALVGAGPGAEDLLTLRAQRLLMEADVILYDALVPEAVVAMGRRDAERVSVGK
                     RKGCHSKTQAQINTLLVELGREGKRVVRLKCGDPLVFGRAGEEMAALREANIS"
     gene            complement(1304..2053)
                     /locus_tag="VGM46_02210"
     CDS             complement(1304..2053)
                     /locus_tag="VGM46_02210"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY4191434.1"
                     /translation="MADTDTRKTIVLTGASRGIGHATVKRFSREGWRVITCSRQAFAD
                     DCPWPAGPEDHIKVDLADQEDVGIAVSEIRHRLEAQGGQLHALINNAAISPKLEDGGR
                     MNSIDTPMHAWRDVFQVNFFAPIMLARGLFKELAAAKGSIVNVTSIAGTRVHPFAGTA
                     YATSKAALGSLTREMAHDFGPHGIRVNAIAPGEIDTAILSPGTEKIVETIPLRRLGAT
                     AEVADIIFFLCSQQASYVTGSEIHINGGQHV"
     gene            complement(2410..>4680)
                     /locus_tag="VGM46_02215"
     CDS             complement(2410..>4680)
                     /locus_tag="VGM46_02215"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEY4191435.1"
                     /translation="KRTDIRIEPSFEGSAKTKSPDGFSVSEEDRVVPRNRKTSSRDKA
                     SSKGKAAKTRSSRRRSRSSRSGLVGMAARLVYWCFVLAIWGGIAVAGIVVYYGAKMPA
                     ATTWAIPDRAPNIKIVSADDQLIANRGLSGGEAVGLHEMSPYIPQAVIAIEDRRFYSH
                     FGIDPIGLARAMVTNVLGGHFSQGGSTLTQQLAKNLFLKPDRTLERKVQEVLLALWLE
                     HEHSKDQILAMYLNRVYFGSGAYGVEAASRRYFGKSARDVTLPEAALLAGLLKAPSRL
                     SPARDPKAAEERSQLVLAAMRDEGMIDDKQRAGAMSAPATRTPSYWTGSENYVADTVM
                     EELPDLIGDVQGDIVIDTTVDLTLQRLAEKSIRRLIDESGKKLDVTQGALVSIDDSGA
                     VRAMVGGYDYSTSQFDRASEARRQPGSAFKPFVYMAALEAGRTPDSVRNDAPIKIGKW
                     SPDNYGGKYFGKVTLATALAKSLNSVAAQLTMEVGPKAVIEAAHRMGIQSELQDNTSI
                     ALGTSEVTPLELTSAYVPFANGGFKPDIHFISKITDAKGKVLFENKAGNAPRVVRQDI
                     VGMMNSMMTGTVESGTARKAAFAWPSAGKTGTSQNSRDAWFVGYTANLTTGVWFGNDD
                     GTPMKRVTGGALPAQAWHEFMVAAHEGVPVRPLPGTWKSTPSDRIVQDEQPIAAAPAP
                     KPSPSVPVARAVVPASPLPARAVRSAQAADEGGDLAMPGDDGSTASIKRPVPPGEVGG
                     PLKKRETSIIDLLTGG"
BASE COUNT          852 a         1536 c         1460 g          832 t
ORIGIN      
        1 taggaaatat tcgcctcgcg cagcgccgcc atctcctcgc ccgcacggcc gaacaccagc
       61 ggatcgccgc atttgagccg caccacgcgc ttgccttcac ggcccagttc gaccagcagc
      121 gtgttgatct gcgcttgcgt cttggagtgg cagcccttgc gcttgcccac cgagacgcgt
      181 tcggcgtcgc ggcggcccat ggccacgacg gcttcgggca ccaacgcatc ataaaggatg
      241 acatcggctt ccatcagcag gcgctgcgcg cgaagcgtca gcaaatcctc ggcgcccggc
      301 ccggccccca ccagcgcgac atggccggcg gccggcgtgg tggaaaagag caggtcggcg
      361 gcggcgccgt tggcctttgc cggctggccg gcagcgagcg cgcgtgccgg cgcgccggtg
      421 aagaactcgc gccagaagcg gcggcgaaca ttgcccttgg gcagcagcgc ttcggccgca
      481 tcgcgaaacg aagcggcgag cgaggcgagc ggcccgagcg acggcgaaag catctggtca
      541 atacgggcgc ggatcatctg ggcaagcacc ggccccgccc cttccgtgcc gatggcgacc
      601 gtcaccggcg cgcggttgac cagcgccggc gtaaagaaat cgcacagttc cggccggtcg
      661 accgcattga ccggaatgcc cagcgcacgc gcatcgcgcg aaaccatcct atccagttcg
      721 ccgttgccgc tggccgcgaa aaccatggcc gcaccttcga gatgcgaggc aagataagcg
      781 gcttcgatat ggatagcctc gttggcgacg atccactggt ggaaatcttc cgacacctgt
      841 tcggcgacaa tgtgcagccg ggcgctggac tggccgacca gccttgcctt ggccagcgcc
      901 tcgtcaccgc cgccgacgat gatcacgtcg cggccttcga ccttgaggaa gatcggaaat
      961 gcattcagct ttggctgggc ggtgggcata aaagcaggtt cctgaataat caggattttt
     1021 acccgcgcgc ggccaagacc agaagaaacc cactcgcgtc ctgcaaggtg ccaggcaatc
     1081 ggttttctga aacccgcggc gggcgcagaa aactattccg ctcggccgcg ccaagcgcgg
     1141 ttatgttcca tttttcacca aaatcatcga ggttcgatgc ctctcgccgg cttcatacga
     1201 aaccgccctg aagcgccggt tctaaaccaa tccacgcccg cgctttggtt gctggtttcg
     1261 agcatgccac ccatcccgaa accgagatca ctttcggcgt atcctagacg tgttgcccgc
     1321 cattgatatg gatttccgag ccggtgacat aggatgcctg ctgcgaacac agaaaaaaga
     1381 tgatgtcggc aacttccgcc gtagcgccca gccggcgcag cgggatggtc tccacgatct
     1441 tttccgtgcc cggcgacagg atcgccgtat cgatttctcc cggcgcgatc gcgttgacgc
     1501 ggatgccgtg cggcccgaaa tcatgcgcca tttcccgcgt cagcgagcca agcgcggcct
     1561 tggaggtcgc ataggccgtg ccggcgaacg gatggacccg cgtgccggca atcgacgtga
     1621 cgttgacgat ggaacccttc gccgcggcca gttccttgaa caggccgcgc gccagcatga
     1681 tcggcgcgaa gaaattgacc tggaatacgt cgcgccaggc atgcatcggc gtatcgatgg
     1741 aattcatgcg cccgccatct tccagcttgg gcgagatggc ggcattgttg atcagcgcat
     1801 gaagctggcc gccctgcgct tcgagccgat gacggatttc cgaaacggcg atgccgacat
     1861 cctcctgatc ggcaagatcg accttgatgt ggtcctcagg tccggccggc catgggcaat
     1921 catcggcgaa agcttgccgc gagcaggtga tgacgcgcca cccctcgcga gaaaagcgct
     1981 ttacggtcgc gtggccgatg cctcggctcg cccccgtcag cacgatggtc tttctggtgt
     2041 cggtgtcggc catgtcgcaa tctccggcgc agacacttag cggaagccgg aagccgtggc
     2101 gaggggcaag tctctcgacg cctgcggcaa accggaacca cggccatcaa cggccacgga
     2161 taaaaccgcc ggaaatcgac aggcaggaac gcctgtcggc aacgcccgcc aatctctcgc
     2221 aggaagtcaa agacttccag ccgtcttcgg caacgaatgg caggcgctgc aatccatgaa
     2281 ctgcagacca tcccgatggc aatcgggcct gcgcaaagca cctgacaatg cgcgatggca
     2341 gatagtccgc ggcaagcagt ctttcgccaa ccaatgggtc ggaagcgtca agcctggccg
     2401 attatccgct caaccgccgg tcaaaaggtc gatgatcgac gtctcgcgct ttttcagcgg
     2461 tccgcccact tcgccgggcg gcaccggccg cttgatggaa gcggtggagc catcgtcgcc
     2521 gggcatggcg agatcgccgc cttcatccgc cgcctgagcc gagcgcaccg cgcgcgctgg
     2581 caagggcgat gcgggaacga ccgcgcgcgc gacgggaacc gatggcgaag gctttggcgc
     2641 cggcgcggcc gctatcggct gttcgtcctg gacgatacgg tctgacgggg tggatttcca
     2701 tgtgccgggc aacggccgca ccggcacgcc ttcatgcgcg gccaccatga actcgtgcca
     2761 tgcctgcgcc ggcagcgcgc cgccggtgac ccttttcatc ggcgtgccat cgtcattgcc
     2821 gaaccacacg ccggtggtca ggttggcggt gtagccgacg aaccaggcgt cgcgcgaatt
     2881 ctggctggtg ccggtcttgc cggccgacgg ccacgcgaaa gccgcctttc gggcggtgcc
     2941 tgactcgacg gttcccgtca tcatcgaatt catcatgccg acaatatcct gccggacgac
     3001 gcgcggggca ttgccggcct tgttctcaaa cagcaccttg cccttggcgt cggtgatctt
     3061 gctgatgaaa tggatatccg gcttgaaacc accgttggcg aatggaacat aggccgaagt
     3121 gagttcgagc ggcgtaacct ccgacgttcc aagcgcgatc gacgtattgt cttgcagttc
     3181 cgactggatg cccatgcggt gcgccgcctc gatcaccgcc ttggggccga cctccatggt
     3241 aagctgcgct gccaccgaat tcagcgattt cgccagggcg gtcgccagcg tcaccttgcc
     3301 gaaatatttg ccgccataat tgtccggcga ccatttgccg atcttgatcg gcgcgtcgtt
     3361 gcgcacgctg tcgggcgtgc ggccggcttc caacgcggcc atgtagacga acggcttgaa
     3421 ggccgaaccg ggctggcgtc gcgcctcgga agcgcggtcg aactggctgg tcgaatagtc
     3481 ataaccgccg accatggcgc gcaccgcacc ggaatcgtcg atggaaacca gcgcaccctg
     3541 ggtcacgtcg agcttcttgc cgctttcgtc gatgaggcgg cggatcgact tttcagcaag
     3601 ccgctgcaaa gtcaggtcga cggtggtgtc gatgacgata tcgccctgca cgtcgccgat
     3661 caggtcgggc aactcctcca tcaccgtatc ggcgacgtag ttttccgaac cggtccagta
     3721 ggaaggcgtg cgcgttgccg gcgcgctcat ggcgcccgcg cgctgcttgt cgtcgatcat
     3781 gccttcgtcg cgcatggcgg ccagcaccag ttgcgagcgt tcttccgccg ctttcgggtc
     3841 gcgcgccggc gacagcctgg acggcgcctt caaaagcccg gccagcaaag ccgcctccgg
     3901 caaggtaacg tcgcgcgcgc tcttgccgaa atagcggcgc gaagccgcct cgacgccata
     3961 ggcgcccgag ccgaaataga cgcgattgag gtacatcgcc aatatctggt ccttgctgtg
     4021 ctcatgctcc agccacagcg ccagcagcac ctcctgcacc ttgcgttcca gcgtgcggtc
     4081 gggcttcagg aacaggttct tggccaattg ctgggtaagc gtcgagccgc cctggctgaa
     4141 atgcccgcca agcacgttcg tcaccatcgc gcgggcaagg ccgatcggat cgatgccgaa
     4201 atgggaatag aaacggcggt cctcgatggc gatgaccgcc tgcgggatgt agggcgacat
     4261 ttcatgcagg cccaccgcct cgccgccgct caagcctcgg ttggcgatca gctggtcgtc
     4321 ggcggaaacg atcttgatgt tgggcgcgcg gtcgggaatg gcccatgtcg tcgcggcagg
     4381 catcttggcg ccgtaataga cgacgatgcc ggcaaccgcg atgccacccc agatggccag
     4441 cacgaagcac cagtatacca ggcgagccgc catgccgacc agcccgcttc gcgaacttct
     4501 gcttcggcgg cgcgacgatc tggtcttggc cgccttaccc ttggaagaag ccttgtccct
     4561 ggaagaggtc ttgcggttcc tcggcacgac gcggtcctcc tcgctcaccg agaagccgtc
     4621 cggggacttc gtcttggccg acccctcgaa gctgggctcg atgcgtatgt ctgtgcgttt
//
LOCUS       DASYCS010000060         4924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3636588,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000060 DASYCS010000000
VERSION     DASYCS010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4924
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3636588"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="VGM46_02220"
     CDS             complement(<1..444)
                     /locus_tag="VGM46_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEY4191436.1"
                     /translation="MPQAPFTAMSLCNNATLRRATRKLGQFYDDALAPSGLRATQQGL
                     LYLIKAIDEPAMGVLADALVMDLSALGHTLKPLIRDGLVETFADTQDRRVKRVRLTAA
                     GSEKLDVALKLWMVAQQHVEELMGKEQAARLRETLDTIAQLDFGKI"
     gene            694..1485
                     /locus_tag="VGM46_02225"
     CDS             694..1485
                     /locus_tag="VGM46_02225"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013530115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY4191437.1"
                     /translation="MSGQDKGTAVVTGASSGLGAIYADRLAKQGHDLVLVARRADRLE
                     ALAKDIRAASGRKVTVIVANLADDGDLRRVEQAISADDSVSVLVNNAGIGGQAVLAEA
                     DADDAERQIKVNILALTRLTRAVLPGMIARDRGAIVNIASVLAFDVSYGGIYSGAKAY
                     VVNFTEGLQAELAGTKVAAQIVAPGPIRTEFWDVAGASLSSLPESIIMTGDDLVDAAL
                     AGLAKGEGFTAPGLANAEAWDAYKGARNGFYGQLIAGKPAARYGV"
     gene            complement(1555..2166)
                     /locus_tag="VGM46_02230"
     CDS             complement(1555..2166)
                     /locus_tag="VGM46_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018114418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY4191438.1"
                     /translation="MRTIKASTFISLDGVMQAPGGPEEDPVAGFKFGGWTVPFWNEDG
                     MDDIMDDIFSKPYDLLLGRKTYDIFADYWPRHADNPIGEQFNAATKYVATSSPDTLAW
                     QNSRPLGNDIIGALRELKKQDGPDLLLQGSSQLAHALFASDLIDKFTLLVFPVLLGKG
                     KRLFDDKAMPVTLRQTRSHTSSSGVFSATYERAGEVKTGSFAD"
     gene            complement(2266..3633)
                     /locus_tag="VGM46_02235"
     CDS             complement(2266..3633)
                     /locus_tag="VGM46_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEY4191439.1"
                     /translation="MPEAELAAPQQSAWHFFQRQDGERPSLPEVNSSVAVPGSGSWMR
                     RLFAFMGPGYMISVGYMDPGNWATDIAGGAQFGYTLLFVIMLSNLMAILLQALAARLG
                     IATGRDLAQACRAYYPRPVAYVLWVACELAIIACDLAEVIGTAIALNLLFGIPLIGGA
                     LLTALDAFLVLLLMNKGFRYLEAFVVALLVIIFGCFAVQIFVAAPPVGSILHSMFVPS
                     SEIATNPAMLYIAIGIIGATVMPHNLYLHSSIVQTRAYERTDHGKRDAIKWATLDSTI
                     ALVLALFVNASILIVAAVAFHGTGHQDVAEIGQAFQLLSPLLGLGIASILFAVALLAS
                     GLNSTVTATLAGQIVMEGFLHLRIPQWARRLLTRGLAIIPVVVVAALYGEKGTAELLV
                     FSQVVLSMQLPFAVIPLVMFVSDKKKMGNFAIARSVAVLAWIVACLILVLNFKLLYDT
                     AIGFG"
     gene            4369..4824
                     /gene="mntR"
                     /locus_tag="VGM46_02240"
     CDS             4369..4824
                     /gene="mntR"
                     /locus_tag="VGM46_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015915557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HEY4191440.1"
                     /translation="MTPNKRPIPRDEPLPDAEIHSEGFRQTRVAQRSALVEDYVELIA
                     DLIEDGNEARQVDIAARLGVAQPTVAKMLTRLCADGLVSRKPYRGVFLTEAGRKMAEE
                     SRIRHQTVEAFLRSLGVSAETARIDAEGIEHHVSGETLEAFRRAMTKAH"
BASE COUNT          955 a         1398 c         1677 g          894 t
ORIGIN      
        1 tatcttgccg aagtccagct gggcgatggt gtccagcgtc tcgcgaaggc gtgcggcctg
       61 ttccttgccc atcagttctt ccacatgctg ctgggcaacc atccaaagct tgagggcgac
      121 gtcaagcttt tcgctgcccg cagccgtcag ccttacgcgc ttgacgcggc ggtcctgagt
      181 gtcggcgaat gtctcgacaa ggccatcgcg gatgaggggc ttcagcgtgt ggccgagcgc
      241 tgacaggtcc atgacaagcg cgtcggcaag gacgcccatg gcgggctcgt cgatagcctt
      301 gatcaggtag agcaggccct gctgggtagc gcgcaggccc gaaggcgcca gcgcatcatc
      361 atagaactgg ccgagctttc gcgtggcgcg ccgcagcgtg gcgttgttgc aaaggctcat
      421 ggccgtaaag ggggcttgcg gcatttccgt tttactcgtt ggtggcaggc aggatcgcct
      481 gccggctcat cgtgggcaag cgggattgcc tgccggaacg cttaaaatag tttttgccgg
      541 caacattgac aagatagtgg catatgccta tttacctatt agtggcatat accactaaat
      601 taattggttg ccggaagcac cgaacgtaag tcccgccatt tggcggcatg cgagatcgct
      661 gctccggata aacaggaaaa aatggagcga aagatgagtg gacaagacaa gggtacggcg
      721 gttgtgaccg gcgcgtcgtc gggattgggt gcgatctatg cggaccgcct ggcaaagcag
      781 ggccatgatt tggtgctggt ggcgcggcgt gccgacaggc tggaagcgct ggcgaaggac
      841 atccgcgccg cttccggcag gaaggtcacg gtcatcgttg ccaatctcgc cgatgacggc
      901 gacctgcgcc gggtcgaaca ggcgatctcg gccgatgaca gcgtatcggt cctggtcaac
      961 aatgccggga tcggcgggca agcggtgctg gccgaagccg acgcggacga tgccgagcgc
     1021 cagataaagg tcaatatcct agcgctgacc cggctgacgc gcgctgtgct gccgggcatg
     1081 atcgcgcgtg atcgtggcgc gatcgtcaac attgcctcgg tccttgcctt cgacgtttcc
     1141 tatggcggca tctacagcgg cgccaaggct tatgtcgtca atttcaccga aggtttgcag
     1201 gcggagctcg ccggcaccaa ggtggcggcg cagatcgtcg cgccggggcc gatccgcacg
     1261 gaattctggg atgtcgccgg cgccagcctg tctagcctgc cggaatcgat catcatgacc
     1321 ggcgacgacc tggtggatgc ggccctcgcc ggcctggcca agggggaggg cttcacggct
     1381 ccggggttgg cgaatgccga ggcatgggac gcctacaagg gtgcccgcaa cggtttttat
     1441 ggccagctga tcgccggcaa gcccgcggcg cgctacggcg tctgaaccgt atcaggattg
     1501 aaaatggggg cgggctaatg tccgctccca ttttcgttta tgggcaaatg cctgttagtc
     1561 cgcgaacgag ccggtcttga cctcgccggc acgctcgtag gtcgccgaga agacgccgct
     1621 gctcgatgta tgcgagcgtg tttgcctgag ggtcaccggc atcgccttgt cgtcgaacag
     1681 gcgcttgccc ttgccgagca gcaccgggaa gaccagcagg gtgaacttgt cgatcaggtc
     1741 gctggcaaac agcgcatgcg caagctggct cgacccctga aggagaaggt cggggccatc
     1801 ctgcttcttc aattcacgca gcgcgccgat aatatcgttg cccagcggcc tggagttttg
     1861 ccacgccagc gtgtcgggcg acgatgtcgc cacatatttg gtggcggcgt tgaactgttc
     1921 gccgatgggg ttgtcggcgt ggcgcggcca gtagtcggcg aaaatatcat aggtcttgcg
     1981 gccgagcagc aggtcatagg gcttcgagaa tatgtcatcc atgatgtcgt ccatgccgtc
     2041 ttcgttccag aagggcacgg tccagccgcc gaacttgaag ccggcgacgg ggtcttcttc
     2101 cgggccgccg ggggcctgca tgacaccatc gaggctgatg aaggtggagg ctttgatcgt
     2161 tctcattgga gttcctttcg cggctgtccg ttattgaccg ctctaaggac gaacgaccct
     2221 gccaccatcc gacaggagcg ggaaaaaatc ttgcggaacg cgccttcagc cgaagccgat
     2281 ggcggtgtcg tagagcagct tgaagttcag caccaggatg aggcaggcga cgatccaggc
     2341 aaggacggcg acagagcgcg caatggcgaa attgcccatc ttcttcttgt ccgaaacgaa
     2401 catcaccaac gggatgaccg caaagggcag ttgcatggag agcaccacct ggctgaacac
     2461 cagcagctcg gcggtgccct tttcgccgta gagcgccgcc accaccacca cggggatgat
     2521 ggccaggccg cgcgtcagaa ggcggcgcgc ccattgcgga atgcgcaggt gcaggaagcc
     2581 ttccatgacg atctggccgg ccagcgtcgc ggttaccgtg gagttcaggc cggaggcgag
     2641 cagcgccacc gcaaacagga tggaagcgat accgagacca agcaacggag acaaaagctg
     2701 gaacgcctgg ccgatttcgg cgacatcctg atggccggtg ccgtggaagg cgacggcggc
     2761 gacgatcagg atggaggcgt tgacgaacag cgccagcacc agggcgatgg tggaatccag
     2821 cgtcgcccat ttgatggcgt cgcgcttgcc gtggtcggtg cgctcatagg cgcgggtctg
     2881 cacgatggat gagtgcaaat agaggttatg cggcatgacg gtggcgccga tgatgccgat
     2941 ggcgatgtag agcatggccg ggttggtggc gatctcgctg gacggcacga acatggaatg
     3001 caggatcgag ccgaccggtg gagccgccac gaagatttgc accgcaaagc agccgaaaat
     3061 gatgacgagc agcgcgacaa cgaaagcttc gagatagcga aagcccttgt tcatcagcaa
     3121 aagcacgagg aaggcgtcga gcgcggtgag cagggcgccg ccgataagcg ggatgccgaa
     3181 caaaagattg agcgcgatgg ccgtgccgat gacttcagcc agatcgcagg cgatgatggc
     3241 cagttcgcag gcaacccaca gcacataggc gacgggccgg gggtaatagg cgcggcacgc
     3301 ttgcgcgagg tcgcgccccg ttgcgatgcc gaggcgggcg gccagcgcct gcaacaggat
     3361 cgccatcagg ttcgacagca tgatgacgaa cagcagcgtg tagccgaact gcgcgccgcc
     3421 ggcgatgtcg gtcgcccagt tgccggggtc catatagccg accgagatca tgtagcccgg
     3481 acccatgaag gcgaacaggc ggcgcatcca actgccggaa cccggcaccg cgacggagga
     3541 attgacctcc ggcaagctcg gcctttcgcc gtcctggcgc tggaagaaat gccaggcgga
     3601 ttgctgcggt gcggcgagtt cggcttcagg catgactaac ccaggtcgga ttgcagttct
     3661 ctggcgttaa tttatgcaca ggctcaacat tatgcaatag gctatatttc attgttttgg
     3721 ctttatggaa gcggtgggcg gggcatgggg cagggaatga ctggcgaggt cgaagcgggt
     3781 cgagatcgag ctgcatgggc aaaggcgcag ccaccgtcgc ggcatcgatc ccggatagcc
     3841 cagccgtcgc tggcgctccg gcacggcttc cgggatgacg acgtttgggg tgggctgttg
     3901 tggcggttat gggacgcggg ccttcggtgg cagcggccgg actgtgagcg gcgcaacact
     3961 ctccttcgtc atcacgacca aatgagcggc agtcgagcga ggagcgggat tcatgccgtg
     4021 ccattgccga gtgttggcga tttgcccgat cggcggcatc atgagatggc ctcggatagc
     4081 tgtaccgtcg ctggtgttcc ggttcggttt gccgatcacg atgatgggat gattatcggg
     4141 tgacgcccag ggggctgctg atggtggggc tggagctgtt gtgaggcccg acatttctgc
     4201 ttggcgtgag ttggctggaa aggccgttcg ggccggttca tgcggccgag ccagttgccg
     4261 caccgcgcat aaacgctctc gaaacataaa agcccgtgct ataaatgtta aagaccgatg
     4321 ctatcttccg cttcggttgc ctgttgccag cagttttgga gatgcgcatt gacgccgaac
     4381 aaacgaccca taccgcgcga tgagccgctg cccgacgccg aaatccattc ggaaggattc
     4441 cggcagacgc gcgtggcgca gcgcagcgcg ctggtcgagg attatgtcga gttgatcgcc
     4501 gacctgatcg aggacggcaa cgaggcgcgc caggtggata tcgccgcgcg tctcggcgtg
     4561 gcgcagccga ccgtcgccaa gatgctgacg cggctttgcg ccgacgggct ggtttccaga
     4621 aagccctatc gcggcgtttt cctgaccgag gcggggcgca agatggccga ggaaagccgc
     4681 atccgccatc agacggtcga ggcgttcctg cgttcgctcg gcgtcagcgc cgaaaccgcg
     4741 cgcatcgatg ccgagggtat cgagcaccat gtcagcggcg agacgctgga ggcgttccgc
     4801 cgcgccatga ccaaggcaca ttgactgtcc gcatggccgc accaggcctg ccaggcggcg
     4861 ccggaaccgc cgtatcgacc atgcgttgac tgtgatgccc gcggttggcg cgggccagcg
     4921 gagg
//
LOCUS       DASYCS010000061         8697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2476774,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000061 DASYCS010000000
VERSION     DASYCS010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 8697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8697
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2476774"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1400)
                     /locus_tag="VGM46_02245"
     CDS             complement(<1..1400)
                     /locus_tag="VGM46_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010658275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY4191441.1"
                     /translation="MTKHMPNLKAAKITGGVAASQRHDSAHKHVSGTAVYIDDLPEPA
                     GTLHLGVGLSTVAHGLLKSVDLSAVRAAPGVVDVLTWKDVPGVNDISPSGMHDDPVLA
                     QDKVLFHGQPIFCVLAKTREQARHAARLARIEYEELPAIIDIWDLDSAKDRQVTTPLI
                     LKRGDAAAAIAAAPRRIKGRMKLGGQDHFYLEGQVSLAVPGEDDEVTVHCSTQGPSET
                     QHMIAHALGVPSHAVAVEVRRMGGGFGGKETQANQCAVLAAIAAKKHNRAVKFRMDRD
                     EDMVATGKRHDFAIDYDVGFDGEGNILGIDYTFALRCGFSADLSGPVGDRALFHCDNA
                     YFFPAVHARSAPLFTNTVSNTAFRGFGGPQGMVGAERVIDEIAFAVGKDPLEIRKLNF
                     YDPMGKEAGRNVTPYHQKVEDCIIQRIVAELEESADYAGRRREIAAFNANSRFIKRGL
                     ALTPVKFGISFTKTES"
     gene            complement(1400..2881)
                     /gene="xdhA"
                     /locus_tag="VGM46_02250"
     CDS             complement(1400..2881)
                     /gene="xdhA"
                     /locus_tag="VGM46_02250"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase small subunit"
                     /protein_id="HEY4191442.1"
                     /translation="MQKSELRTELRFILNGGDVVLADVAPDATLLDWLRLERSLRGTK
                     EGCAEGDCGACTVLVGKLSANGLVYESVNACIRFMGSLDRCHVVTIEHLRGEGQALHP
                     VQQAMVDFHGSQCGFCTPGFVMSLYGLWMRQPDPSNAAIEKALQGNLCRCTGYEAIIR
                     AARAISSYGKAQDDPLRVERQAVTARLKALDDGSRVEIGSGKKRLIVPANVDDFATLL
                     EAEPAATVVAGSTDVGLWVTKQMRDISPVIFIGNLDGLGELSVEDGAINIGAGVTYTE
                     AFATLAQHIPALGPLFDRIGGEQVRNMGTIGGNIANGSPIGDTPPPLIALGASLTLRK
                     GKQRRTIQLEKYFIAYGKQDRQPGEFVEKLTVPVPKANEKFAVYKITKRRDEDITAAL
                     AAFFLRLAQDGTVAEARIAYGGMAATPKRASAVEKALTGKPWTEETVMAAMAEYATDY
                     SPLTDMRATAEYRSMAARNLLLRFYAETSGTKEPIQVSRHEAA"
     gene            complement(2885..3256)
                     /gene="uraH"
                     /locus_tag="VGM46_02255"
     CDS             complement(2885..3256)
                     /gene="uraH"
                     /locus_tag="VGM46_02255"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HEY4191443.1"
                     /translation="MANASSGGGSLTTHVLDTATGKPAAGLSITLYRVSGNSHRKLKT
                     VVTNADGRCDEPLLEGKDFQLGQYELIFSVGNYFRSLGLQLPETPFLDEVPIRFGMAQ
                     ATHYHVPLLVSPYGYSTYRGS"
     gene            3426..4859
                     /gene="puuE"
                     /locus_tag="VGM46_02260"
     CDS             3426..4859
                     /gene="puuE"
                     /locus_tag="VGM46_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013891598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase PuuE"
                     /protein_id="HEY4191444.1"
                     /translation="MRYPRDMRGYGANPPDPKWPGGAHVAVQFVVNYEEGGENCVLHG
                     DKASEAFLSEITGAAPWQGMRHWNMESIYEYGARAGFWRLHRLFTEKNVPATCYGVAS
                     ALARSPDQLTAMQEAGWEIASHGLKWIDYRDHSPDDERRDIEEAIRLHTEVTGERPTG
                     WYTGRTSDNTVRLVAEAGGFAYCSDTYDDDLPYWLNHDGHGSAIEPQLIIPYTLDAND
                     MRFGIQAGFSNGEQFLTYLKDSFDTLYEEGKAGRPKMMTIGLHCRLIGRPGRFGALKR
                     FIEYVQSHDKVWLARRIDIANHWREHHPYEAAALRPSRMDRADFVKSFGGVFEHSPWV
                     AEKAFAMELGAAHDSAGGLHNALCRAFRSASEAERIKVLQAHPDLAGKIAMAKQLTPE
                     STREQASAGLDALTDKERELFSRLNAAYVTNFGFPFIIAAKGLGKDEILSRFETRIGN
                     DRETEFKTACKQVERIALLRLRDMLPK"
     gene            4871..5719
                     /locus_tag="VGM46_02265"
     CDS             4871..5719
                     /locus_tag="VGM46_02265"
                     /EC_number="3.5.3.26"
                     /EC_number="3.5.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004037 - allantoicase activity [Evidence
                     IEA];
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional allantoicase/(S)-ureidoglycine
                     aminohydrolase"
                     /protein_id="HEY4191445.1"
                     /translation="MDKMTMAGHNYYAPTGGLPPQTQLLTGRAVFTQAYAVIPKGVMS
                     DIVTSPLPHWEKTRAWIIARPLSGFAETFSQYIMEVLPGGGSDRPEPNAGAEGILFVV
                     EGELTVTLDGKKHRLNPGGFAFIPPSSDWQVRNEGAGPTRYHWIRKAYEAVDGIDAPE
                     AVFADEADIAPSPMPDTNGAWATTRFVDPADLRHDMHVTIVTLKPGGVIPFAETHVME
                     HGLYVLEGKAVYRLNQDWVEVEAGDYMWLRAFCPQACYAGGPGNFRYLLYKDVNRHAK
                     LGGFGH"
     gene            5722..6228
                     /locus_tag="VGM46_02270"
     CDS             5722..6228
                     /locus_tag="VGM46_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006325399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HEY4191446.1"
                     /translation="MHRISAQPLTREAFAPFGEVIDTEGGVHYPINDGKCERYHALAR
                     AEAGGPNGHVIINLFKATPYAFPLKLSMVERHPFGSQAFVPLSPQPFLVVVCADGPDG
                     PGTPSAFVTRPGQGVNYRRNLWHGVLTPIGEAQDFVVIDRGGDGSNVEEFYFSQPYEI
                     HLPESLGS"
     gene            6225..6818
                     /locus_tag="VGM46_02275"
     CDS             6225..6818
                     /locus_tag="VGM46_02275"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015314322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEY4191447.1"
                     /translation="MTDISLIERLLDVIEDDIVPKTAEGVKQGNKLFGAAILRKEDRS
                     LVLAETNNEIENPLWHGEIHCLKRFYEMPKAERVDTGEAIFLATHEPCSLCLSAITWA
                     GFDNFFYLFSHEDSRDSFAIPHDLKILKEVFTLDPGGYNAENAYWKSFSIRKLVRLLP
                     DAERARLEARIGAISARYDELSDAYQDNKADNDIPLN"
     gene            7074..8663
                     /locus_tag="VGM46_02280"
     CDS             7074..8663
                     /locus_tag="VGM46_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY4191448.1"
                     /translation="MSNELEFLSRHVAAGKLSRRDFLGRAAALGVGAAFANTLLSDAA
                     RAAGPVKGGMLKAGLQGGESSNVLDPALNLSQVNFSFGKAWGELLVELNPDGSLENRI
                     AESIGSSPDAKTWTMKIRKGIEFHDGKTVKAEDVVATLERHSDEKSKSAALGILGGID
                     KMKADGDEVVITLKDPNADFPYLMADYHLVIQPNGGKDNPNAGISAGPYKVTVNEPGV
                     RYGGEKFANYWQGDKLGHAAQIEIVVINDATARTAALQGGQVNMINRVEPKIVDLVKR
                     VPGVTIQNVAGRGFYPFNMFCDTAPFDSNDLRMALKLAMDREELLDKILRGYGSVGND
                     IPVNEAYPLFPTDLEQRKFDPEKAAEFYKKSGHSGSILLRTSDVAFPGAVDAAQLYQQ
                     SCAKAGIKVEIKREPGDGYWSEVWNKQPFSLSYWGGRATQDQMYSTAYVSSADWNDTR
                     WKRPEFDKMVLAARGELDQDKRKKMYHDMAVVMRDEGGLIVPFFYQYIDASGKGVQGW
                     MNNPAQEMCNGYALSLCWLEA"
BASE COUNT         1673 a         2708 c         2721 g         1595 t
ORIGIN      
        1 ttggactcgg tcttggtgaa ggaaatgccg aacttgaccg gggtaagcgc cagcccacgc
       61 ttgatgaagc ggctgttggc gttgaaggcc gcgatctcgc gccgccggcc ggcataatcg
      121 gcgctttcct caagctcggc cacgatgcgc tggatgatgc agtcctcgac cttctgatgg
      181 tagggcgtga cgttgcggcc agcctccttg cccatcgggt cgtagaaatt cagcttgcgg
      241 atttcgagcg ggtccttgcc gaccgcgaag gcgatctcgt cgatgacgcg ctcggcgccg
      301 accatgccct gcgggccgcc gaaaccgcga aaggcggtgt tcgaaaccgt gttggtgaac
      361 agcggggccg agcgcgcatg cacggccggg aagaaataag cgttgtcgca atggaacagc
      421 gcccggtcgc cgaccggacc cgacaggtcg gctgaaaacc cgcagcgcag cgcgaaggta
      481 tagtcgatgc cgagtatgtt gccctcgccg tcgaaaccga catcgtagtc gatggcgaaa
      541 tcgtgccgct tgccggtggc gaccatgtcc tcgtcgcggt ccatgcggaa ttttaccgcg
      601 cggttgtgct ttttcgccgc gatcgccgcc agcacggcgc actggttggc ctgcgtttcc
      661 ttgccgccga aaccgccgcc catgcgtcgc acttcgacag ccacggcatg gctcggcacg
      721 cccagcgcat gcgcgatcat gtgctgggtt tcgctcggcc cctgcgtcga gcaatggacg
      781 gtcacctcgt cgtcctcgcc cggcacggcc agcgaaacct ggccttcgag gtagaaatgg
      841 tcctgcccgc caagcttcat gcggcctttg atgcggcgcg gcgcggcggc gatggcggcg
      901 gcggcgtctc cccgcttgag gatcagcggc gtggtgacct gcctgtcctt tgcggaatcg
      961 agatcccaga tatcgatgat cgccggaagt tcctcatatt cgatcctggc caggcgggcg
     1021 gcatggcggg cctgctcacg cgtctttgcc agcacgcaga aaatcggctg gccgtggaac
     1081 agcaccttgt cttgcgccag caccgggtcg tcatgcatgc cgcttggcga aatgtcgttg
     1141 acgccgggaa cgtccttcca ggtcagcacg tcgaccacgc cgggcgcggc gcgcaccgcg
     1201 gacaggtcga ccgatttcag caagccatgc gcgaccgtgg aaagaccgac gccaagatga
     1261 agggtgccgg ccggctccgg caggtcgtcg atatagacgg cggtgccgct gacatgcttg
     1321 tgcgccgaat cgtgacgctg gctggcggcg acgccgccag tgatcttcgc ggccttgaga
     1381 ttaggcatat gcttggtcat catgccgcct cgtgccgtga aacctggatg ggttctttcg
     1441 tgccgctggt ttcggcgtaa aagcgcaaca gcaggttgcg cgccgccatc gaccggtatt
     1501 cggcggtggc gcgcatgtcg gtgagcggcg aatagtccgt ggcatactcg gccatcgccg
     1561 ccatgaccgt ctcctcggtc cagggcttgc cggtcagggc cttttcaacg gccgaagctc
     1621 gcttcggcgt cgccgccatg ccgccatagg caatgcgggc ttcggcaaca gtgccatctt
     1681 gcgcaagccg caggaaaaac gcggccaacg ccgccgtaat gtcttcgtcg cggcgcttgg
     1741 ttatcttgta gacggcgaat ttctcgttcg ccttcggcac cggaacggtg agcttttcga
     1801 cgaattctcc cggctggcga tcctgcttgc cataggcgat gaagtatttt tccaattgaa
     1861 tggtgcgtct ctgcttacct tttcgcagcg tgagcgatgc gcccaatgcg atcaagggcg
     1921 gcggggtgtc gccgatcggc gagccgttgg cgatgttgcc gccgatcgtg cccatgttgc
     1981 gcacctgctc gccgccgata cggtcgaaga gcggtccaag cgccgggata tgctgcgcaa
     2041 gcgtggcaaa cgcctcggta taggtgacgc cggcgccgat gttgattgcg ccatcctcga
     2101 ccgagagttc gccaagcccg tcgagattgc caatgaagat gaccggcgaa atatcgcgca
     2161 tctgcttggt tacccacagg ccgacatcgg tcgagccggc gaccacggtc gcggccggtt
     2221 cagcctcaag caaagtggcg aaatcgtcga cattggcggg cacgatcagc cgtttcttgc
     2281 cggagccgat ttcgacgcgg gaaccatcgt ccaacgcctt cagcctggcg gtaaccgcct
     2341 ggcgctcgac gcgcagcgga tcgtcctgcg ccttgccata gctggagatg gcgcgggccg
     2401 cgcggatgat ggcttcataa ccggtgcagc ggcagagatt gccctgcaat gccttttcaa
     2461 tcgccgcgtt ggatggatcg ggctggcgca tccacaggcc ataaagcgac atgacgaagc
     2521 ccggcgtgca gaagccgcat tgcgagccgt ggaaatccac catcgcctgc tgcaccgggt
     2581 gcagcgcctg cccctcgccg cgcaaatgct cgatggtgac gacatggcag cggtcgagcg
     2641 aacccataaa acggatgcag gcattgacgc tttcgtaaac gaggccgttt gccgaaagct
     2701 tgccgaccag caccgtgcag gccccgcaat cgccttcggc gcagccttcc ttggtgccgc
     2761 gcaacgagcg ctccagccgc agccagtcga gcagcgtcgc gtctggcgca acgtccgcca
     2821 ggacaacgtc cccgccgttg aggatgaagc gcagttcggt gcgcagttcg gatttttgca
     2881 tggtctagct ccccctatat gtggaatagc catagggaga gacaagcaac ggcacatgat
     2941 agtgggtcgc ctgcgccatg ccgaagcgga tcggcacttc gtcgaggaac ggcgtttctg
     3001 gaagctggag gccaagcgag cgaaagtaat ttccgaccga gaagatcaat tcgtactggc
     3061 caagctggaa gtccttgcct tcgagcagcg gctcgtcgca acgtccgtcg gcgttggtca
     3121 caacggtttt cagcttgcgg tgcgaattgc cgctgacccg gtagagcgtg atcgaaaggc
     3181 ccgccgccgg cttgccggtg gccgtgtcca gcacatgggt tgtgaggctg ccgccgcccg
     3241 atgatgcatt cgccatgacc gcctcccttg tcgcctttcg ggcatatcga tgaattgtgc
     3301 gccatttaac ggcgaagcat aaggtgcagt cgagcatctg gccccaaaaa cactttcaaa
     3361 attttcgggg aaatgccacc gatcccttta gattatcctg atggcgattt cggcgcggag
     3421 aaaacatgcg ttatcccaga gacatgcgtg gctatggcgc caatccgccg gacccgaaat
     3481 ggccgggcgg cgcgcatgtc gccgtgcaat tcgtcgtcaa ctacgaggaa ggcggcgaaa
     3541 actgcgtcct gcatggcgac aaggcgtccg aggcgttcct gtcggagatc accggcgccg
     3601 cgccgtggca gggcatgcgg cattggaata tggaatcgat ctacgaatat ggcgcgcgcg
     3661 ccggcttctg gcggttgcac cggctgttca ccgaaaagaa cgtgcctgcc acctgctacg
     3721 gcgtcgccag cgcgcttgcg cgttcgccgg accagcttac ggccatgcag gaagccggct
     3781 gggagatcgc ctcgcatggg ctgaaatgga tagactaccg cgaccattca cccgatgacg
     3841 agcgccgcga catcgaggaa gcgatacgcc tgcatacgga agtgaccggc gaacggccaa
     3901 ccgggtggta cacgggccgc acgtccgaca atacggtgcg gctggtcgcc gaggccggcg
     3961 gctttgccta ttgctccgac acctatgacg acgacctgcc ctattggctg aaccatgacg
     4021 gccatggcag cgccatagag ccgcaactga tcattcccta tacgctcgac gccaacgaca
     4081 tgcgctttgg catccaggcc ggtttctcga atggcgagca gttcctgacc tacctgaaag
     4141 acagtttcga cacgctttac gaggaaggca aggccgggcg gccgaagatg atgaccatcg
     4201 gcctgcactg ccgccttatc gggcggcccg gccgcttcgg cgcgctgaag aggttcatcg
     4261 aatatgtcca gagccacgac aaggtgtggc tggcgcggcg catcgacatc gccaatcact
     4321 ggcgcgagca ccacccttac gaagcggcgg cgcttcgacc gtcgcgcatg gaccgcgccg
     4381 acttcgtgaa aagcttcggc ggcgtgttcg aacattcgcc atgggtcgcc gaaaaggcgt
     4441 tcgccatgga actgggcgcg gcgcatgaca gcgccggcgg gctgcacaat gcgctttgcc
     4501 gcgccttccg ctcggccagc gaggcggagc gcatcaaggt gctccaggcg catcccgatc
     4561 ttgccggaaa gatcgccatg gccaagcaac tgacgccgga atcgaccagg gagcaggctt
     4621 ccgccgggct ggacgcgctg accgacaagg agcgcgaact gttttccagg ctcaacgcgg
     4681 cctatgtcac caatttcggc tttccattca tcatcgccgc caagggcctt ggcaaggacg
     4741 aaatccttag ccgtttcgaa acccgcatcg gcaatgaccg cgaaacggag ttcaagaccg
     4801 cctgcaagca ggtggagcgc atcgcgctgc tgcgccttcg agatatgctg ccgaaatagg
     4861 tgtcgaaccg atggataaga tgacaatggc cggccataat tactacgccc caaccggtgg
     4921 cctgccgccg cagacgcagc ttttgaccgg ccgcgccgtt ttcacgcaag cctatgccgt
     4981 catcccgaaa ggggtgatga gcgacatcgt gacaagcccg ctgccgcatt gggagaaaac
     5041 ccgtgcctgg atcatcgcgc ggcctttgtc gggttttgcc gagacgttct cgcaatacat
     5101 catggaggtg ttgccgggcg gcggttcgga ccggcctgag ccgaatgccg gcgcggaggg
     5161 catcctattc gtggtcgagg gcgagctgac cgtcacgctg gacggcaaga agcaccggct
     5221 gaaccccggc ggcttcgcgt tcatcccgcc gtcaagcgat tggcaggtgc gcaacgaggg
     5281 cgccggccct acccgctatc actggatccg caaggcttat gaagccgtcg acggcatcga
     5341 cgcgcccgaa gccgtcttcg ccgacgaggc cgacattgcg ccatcgccga tgcccgacac
     5401 caacggggcg tgggccacga cgcgcttcgt cgatccggcc gatttgcgcc acgacatgca
     5461 tgtcaccatc gtcacgctga agccgggcgg ggtcattccc ttcgccgaga cgcatgtgat
     5521 ggagcacggg ctttacgtgc ttgaaggcaa ggcggtctac cgcctgaacc aggactgggt
     5581 agaggtcgag gccggcgact atatgtggct gcgcgccttc tgcccgcagg cctgctatgc
     5641 gggcggcccc ggcaatttcc gctacctgct ttacaaggac gtcaaccggc acgccaagct
     5701 tggcggattt ggacattgaa catgcaccgc attagcgccc agccgctgac ccgcgaggcc
     5761 ttcgccccct tcggggaggt catcgacacc gaaggcggcg ttcattatcc catcaatgac
     5821 ggcaagtgcg aacgctacca cgccctggcg cgggcggaag ccggcggacc gaacggccat
     5881 gtcatcatca acctgttcaa ggcgacgccc tatgcgtttc cattgaagct ttcgatggtg
     5941 gagcgtcatc catttggcag ccaggccttt gtgccgctgt ctccccagcc gttcctggtg
     6001 gtggtctgcg ccgacgggcc ggatggtccc ggtacgccga gcgcgttcgt cacgcggccc
     6061 ggccaaggcg tgaactaccg gcgaaatcta tggcacggcg tgctgacccc gatcggcgaa
     6121 gcgcaggact tcgtggtgat cgaccgcggc ggcgacggtt ccaacgtcga ggaattttat
     6181 ttctcgcagc cttatgaaat ccatctgccg gaaagccttg gctcatgacc gacatttcgc
     6241 tgatcgagcg tctgctcgac gtgatcgagg acgacatcgt gccgaagacc gccgagggcg
     6301 tgaagcaggg caacaagctg tttggcgcgg cgatcctgcg caaggaagac cgctcgctgg
     6361 tgctggccga gaccaataac gagattgaaa accccctctg gcacggcgag atccattgcc
     6421 tgaaacggtt ctacgagatg ccgaaagccg aacgcgtcga caccggggag gcgattttcc
     6481 tggccacgca tgaaccgtgc tcgctgtgcc tttcagcaat cacctgggcc ggcttcgaca
     6541 atttcttcta tttattcagc catgaggatt cgcgcgacag cttcgccatt ccgcacgatc
     6601 tgaagatcct gaaagaggtg tttaccctcg atcccggcgg ctacaatgcc gagaacgcct
     6661 attggaaaag tttttccatc cgcaagcttg tacgcctgtt gccggatgcc gagcgggcaa
     6721 ggctggaagc gcggatcggg gccatatcgg cgcgttacga tgaactttcc gacgcgtatc
     6781 aggacaataa ggcggataac gatattccgc tgaactgagt atagcttttt ttcgggcgca
     6841 tccttgcagc gccccgcgcc gctcatgccg tgcagtttga cggcggcaga cgcgatcgcg
     6901 catgcgatct atgtcgggta acgacagttt tccatccgct tggtttgagg cgacgcgcga
     6961 tttacgccgt gacatgtcgc aatcggtctt gcgctgtgat ccagcttgcc gtcattattg
     7021 gctcaagaac aagaagccgg ccacagcgcc gtaccaccag agtgaggaac aatatgtcga
     7081 acgaactcga attccttagc cggcacgtag ccgccggcaa gctgagccgc cgtgactttc
     7141 ttggtcgcgc ggccgcgctc ggcgtcggcg ccgctttcgc caacacgttg ctttccgatg
     7201 cggcgcgcgc cgccggcccg gtcaaggggg gcatgctgaa ggcgggcctg caagggggcg
     7261 agtcctccaa cgtcctcgat cccgctctca acctcagcca ggtgaacttc tccttcggca
     7321 aggcatgggg cgaattgctg gtcgagctga acccggatgg cagcctcgaa aaccgcatcg
     7381 cggaatccat cggctcctcg ccggacgcca agacctggac gatgaaaatc cgcaagggca
     7441 tcgaattcca cgacggcaag acggtgaaag ctgaagacgt ggtggccacg cttgagcgcc
     7501 atagcgatga aaagtcgaaa tcggcggcgc tcggcatcct gggcggcatc gacaagatga
     7561 aggcggacgg cgacgaagtc gtcatcacgc tgaaagaccc gaacgccgac ttcccttacc
     7621 tgatggccga ttaccatctg gtcatccagc cgaacggcgg caaggacaat ccgaatgccg
     7681 gcatcagcgc cggtccctac aaggtgaccg tcaatgaacc gggcgtgcgc tatggcggcg
     7741 agaagttcgc gaattactgg caaggcgaca agctcggcca tgcggcgcag atcgagatcg
     7801 tcgtcatcaa cgacgccacg gcgcgcacgg cggcccttca gggcggccag gtcaacatga
     7861 tcaaccgcgt cgagccgaag atcgtcgatc tggtcaagcg cgtgcccggc gtcaccatcc
     7921 agaacgtggc gggccgcggc ttctatccgt tcaacatgtt ttgcgatacg gcgcccttcg
     7981 acagcaacga cctgcgcatg gcgctgaagc tggcgatgga ccgcgaggag ctgctcgaca
     8041 agatcctgcg cggctacggc tcagtcggca acgacattcc ggtcaacgaa gcctatccgc
     8101 tgtttcccac cgacctcgaa cagcgcaagt tcgatcccga aaaggcggcc gagttctaca
     8161 agaagtccgg ccattccggc tctatcctgc tgcgcacctc cgacgtcgcc ttccccggcg
     8221 cggtcgatgc cgcccagctc taccagcaga gctgcgccaa ggccggcatc aaggtcgaga
     8281 tcaagcgcga accgggcgat ggctactggt cggaagtgtg gaacaagcag cccttctcgc
     8341 tctcctactg gggcgggcgg gcgacgcagg accagatgta ttccacggcc tatgtctcca
     8401 gcgccgactg gaacgacacg cgctggaagc gtcctgaatt cgacaagatg gtgcttgccg
     8461 cacgcggcga actcgaccag gataagcgca agaagatgta tcacgacatg gccgtcgtca
     8521 tgcgcgacga aggcggcctg atcgtgccgt tcttctacca gtatatcgac gcctcgggca
     8581 agggcgtgca gggctggatg aacaatcccg cccaggaaat gtgcaacggc tatgcactga
     8641 gcctgtgctg gctggaggcc tgagcgggcg gcgcgcatgt catctccaat cctgaaa
//
LOCUS       DASYCS010000062        11911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2911574,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000062 DASYCS010000000
VERSION     DASYCS010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 11911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11911
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2911574"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1096
                     /locus_tag="VGM46_02285"
     CDS             <1..1096
                     /locus_tag="VGM46_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015317296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY4191449.1"
                     /translation="ADVTNDLQNNALQATLVVDRDKAANLGVDTDTLRSSLYGGFGTQ
                     QVSTIYGSADSYEVVMELDPKIEWSPERMLAIQIRTASGSLVPLGPFAHVERTAGALT
                     VNQLGQLPAVTVSYNLPQGVALGDTVTRINALKEQIGLPKTISTTFAGTAKTFQDSLA
                     NQGLLVGGAILTIYIVLGILYESFIHPLTILTGLPSAVLGALLALRLAGMDLSVIAII
                     GVLMLIGIVKKNGIMMIDVAIQLRREGRTPAEAIHEACLMRARPIMMTTLAALMGTIP
                     IALGTGASAELRQPLGVAVVGGLLVSQALTLFVTPVLYLYMESFSGWLLSLGPKRKPA
                     PPQIAEIEPGHKPRPEEAAVHKPELVRVGS"
     gene            1396..2700
                     /locus_tag="VGM46_02290"
     CDS             1396..2700
                     /locus_tag="VGM46_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191450.1"
                     /translation="MTKSKTLKIETPDLAMLDIPGFEDDLGDALYASTEDGALPDAED
                     VEQDAQPVDGDVQVAAAAPRQSTATRANLAALAAFDGEMRSASAGLDAMETTMAALGS
                     AHEAARRLLGNLHAGVIRANDLEEANAALLADNRRLAERLEQSTHLQSQLQSTDEVNR
                     RRIELLARDHEDAKLALGKSQLEAIELNAAFAEADGERTALTYELAAKSTAADRLARE
                     NELTRQKLANQQMLNTELEQRHAELERKLEEVISVRKAETAEMSELRMRFDSVDKEYK
                     RLQKQSELSQARLVETQERLMSLEADLDELGNRHGAAAERFRTETETLKTKLEAANRK
                     ALADAEEIASLKLEVGDAVSAANVVSAQLACANGQIDAFRSSSAALTFKRTGQTGGET
                     ITRGPVSYDRDRTAKKAKKPLAETSRTAKPESRVVHPRKEHG"
     gene            complement(2835..4814)
                     /locus_tag="VGM46_02295"
     CDS             complement(2835..4814)
                     /locus_tag="VGM46_02295"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013891364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HEY4191451.1"
                     /translation="MAKATGTATRSKQKVAAKPDRAKDAARTTPAEPVAAKGAPRNAK
                     AKRAPEKPAANNTAASAGKPAAGVAKTAAKAPAKAPVKAVPKALAGAAKIPAPEKPLQ
                     KPWLASYPKNMPAEIGALPTHSIGELLVKACKQFAGQPAFTCMGKSLSYAEMERQSSA
                     FAAWLQSTGMPKGARVALMMPNVLQYPVAMMACLRAGYVVVNVNPLYMPRELEHQLKD
                     SGAEAIVILENFATTLQAVVHNTAIRHVVVASMGDLLGAVKGAIVNLVVRRVKKLVPA
                     WSLPGHIKFNAALKAGEALAFKPVKAAADDIAFLQYTGGTTGVSKGATLLHRNVIANV
                     AQLALWVEDAYTAKPKPAHLTYICPLPLYHIFALTVNALMGMQQGAQNILIPNPRDIP
                     GFVKELQKQPIHIFPGLNTLFNALMNNDEFRKLDFSALRLSLGGGMAVQKGVADRWKQ
                     LTGNHITEGYGLSETSPVATANKCSSPDFTGTIGLPMPSTEIAIRDDDGNDLAPGEVG
                     EICIKGPQVMAGYWNRPDETAKVMTADGFFKSGDIGFMDRAGFTKIVDRKKDMILVSG
                     FNVYPNELEEVVAQHPGVLEVAAIGVPDEHSGEAPKLFVVRKDPNLTEADLIAYCRAN
                     LTGYKRPRFVEFRTELPKTPVGKILRRELRDKSPI"
     gene            5207..6799
                     /locus_tag="VGM46_02300"
     CDS             5207..6799
                     /locus_tag="VGM46_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013891363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY4191452.1"
                     /translation="MKKKLTFAAALLAATVMGGIANAKTLVYCAEAAPEGFDPAPYTA
                     GQTFDASSRTAYNRLVEFEPGKTTIAPGLAESWTISDDGLQYTFKLRPGVKFQTTDYF
                     TPTRDFNADDVVFSFDRQVNKDGPWFQYIPGIAYQYYNDQFGTNITKVEKVDDLTVKF
                     TLKDPAITFIPTLGMDFASIISKEYADKLQADKTPELLNQKPVGTGPYIFVDYQTDAV
                     IRYKANPDYWGGKQKIDDLVFAITTDASVRAQKLKAGECNIASYPAPADIAGLQADPN
                     LKVDEQEGLNVGALMYNTQQKPFDDVRVRKALNMAINKQAIIDSVFEGAGQVAVNPIP
                     PTMWSYNKDVKDDPYDPEAAKKLLDEAGVKDLKMNVWAMPVSRPYMPNARRTAELIQS
                     DFAKVGVTVEIVSYDWGEYLKRASAVDHDGAVIVGWTGDNGDPDNFLSVLLSCSAVGS
                     NNRAEWCNKDFDALINKAKTVSDQAERTKLYEQAQVIFKEQAPWATLAHSKVFMPMQK
                     TVTGFAMDPLGIHRFDGVDIAE"
     gene            6854..7861
                     /locus_tag="VGM46_02305"
     CDS             6854..7861
                     /locus_tag="VGM46_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011950286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY4191453.1"
                     /translation="MLRYILNKIALIIPTFIGITILAFGFVRILPGDPVLVLAGERGI
                     SPERHAELLHQFGFDLPIWQQYWIYLSNIAHGDFGISFTTKTPVLRDFLVRFPATLEL
                     AFFAMIFAIILGVGLGILAAVRRGSWFEQLSMGTALVGYSMPIFWWGLLLIILFSGML
                     HWTPVSGRIDLLYFFKPVTGFMTVDALISGQKGAFRSAVSHLILPTIVLGTIPLAVIA
                     RQTRSAMLEVLGEDYVRTARAKGLSPSRVIGIHAFRNALIPVVTTIGLQIGTLMAGAI
                     LTESIFSWPGIGKWMIDAISKRDYFTVQGGLLLIALSVMAVNLIVDVLYAVINPRIRV
                     Q"
     gene            7863..8786
                     /locus_tag="VGM46_02310"
     CDS             7863..8786
                     /locus_tag="VGM46_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013891361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY4191454.1"
                     /translation="MTNEAPAAAEAATLVKSEDARLQRFSEFWYYFSINKGAVAGLVV
                     FALLVLMAIFAPLLAPHSPDDQFRQSFLTPPAWQDGGSWQFVLGTDAVGRDMLSRLIY
                     GARFSLAIGFVVVITALVSGVILGLLAGYCRGWVDTFIMRVMDIILAFPSLLLALVLV
                     AILGPGLVNAMLAIAFVLQPHFARLTRAAVMAEKNREYVVSAKVAGAGHIRLMFSTIL
                     PNCLAPLIVQATLSFSNAILEASALGFLGMGAQPPTPEWGTMLAEAREFILRAWWVVT
                     FPGLAILITVFAINLIGDGLRDALDPKLKRS"
     gene            complement(8795..9259)
                     /locus_tag="VGM46_02315"
     CDS             complement(8795..9259)
                     /locus_tag="VGM46_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191455.1"
                     /translation="MALLVALAPGLAAAGPAAAQGIEISSAKGAVISISAASLAKLPG
                     IEQDARFQTAKGVSNGHYKGALLWDVLKANGAIAETGHHDDLRQVFAVIGRDGYRIDF
                     SVGEIAPEFGNKPMMIATDVDGKPIADGFRLVVPGDRLGARNVRDVVRIEIR"
     gene            9396..10250
                     /locus_tag="VGM46_02320"
     CDS             9396..10250
                     /locus_tag="VGM46_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013527990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4191456.1"
                     /translation="MPLLEIRNLTVSFDTSTGPFMAVQGIDLSIEPREVLAIVGESGS
                     GKSVSMLAVMGLLPNTATVNADRMTFDGIDLLKIDASERRKIIGKDMSMIFQEPVASL
                     NPCFTVGFQIEEVLRFHLGMGRAERRARAIELMTLVGIPDPAERLNSYPHQMSGGQSQ
                     RVMIAIAIACNPKLLIADEPTTALDVTIQKQILDLLVSLQEKYGMGLIMITHNMGVVA
                     ETADRVIVQYKGRKMEEADVLSLFENPKSNYTRALLSALPDNAVGDRLPTISDMLFEP
                     APSVGGVA"
     gene            10247..11074
                     /locus_tag="VGM46_02325"
     CDS             10247..11074
                     /locus_tag="VGM46_02325"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018094285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEY4191457.1"
                     /translation="MTRVLEGKNITRDYHVSGGLLRPARTVHAVKGISFSVDKGKTLA
                     IVGESGCGKSTLARIITLIDPATSGELLIDGHKVDIAKESLSKEMRRKVQIVFQNPYG
                     SLNPRQKIGDVLAEPLKINTNKPAEERRDLAMAMLKKVGLEEKHFNRYPHMFSGGQRQ
                     RIAIARALMLNPSLLVLDEPVSALDLSVQAQVLNLLADLQDEFQLTYVFISHDLSVVR
                     YIADDVMVMYFGEAVEYGSRDEVFADPKHSYTKALFAATPRADVDSIRARLARKQAA"
     gene            11370..>11911
                     /locus_tag="VGM46_02330"
     CDS             11370..>11911
                     /locus_tag="VGM46_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017274410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY4191458.1"
                     /translation="MTIRFGLLGAGRIGKVHARAIGANPQAKLVAVADALDKAAKEIS
                     TAYGAEVRTIDDIEKAADIDAVVICTPTDTHADLIERFARAGKAIFCEKPIHLDAKRV
                     EKCLAVVKKTGAVLMVGFNRRFDPHFAAVRKAIDEGAIGAVEMVTITSRDPGAPPVDY
                     IKRSGGIFRDMTIHDFDMAR"
BASE COUNT         2205 a         3835 c         3582 g         2289 t
ORIGIN      
        1 cgccgacgtc accaacgatt tgcagaacaa tgcgctgcaa gcgacgctgg tggtcgaccg
       61 cgacaaggcg gccaacctcg gcgtcgatac cgatacgctg cgttcaagcc tctatggcgg
      121 cttcggcacg caacaggttt cgaccatcta cggctcggcc gacagttacg aagtggtcat
      181 ggagctcgat cccaagatcg aatggtcgcc cgagcgcatg ctggccatcc aaatcaggac
      241 cgccagcggc agcctggtgc cgttgggacc tttcgcccat gtcgagcgca ccgccggcgc
      301 gctgaccgtc aaccagctcg gccagcttcc ggccgtcacc gtttcctaca acctgccgca
      361 gggcgtggca ttgggcgaca ccgtcacgcg catcaatgcg ctgaaggaac agatcgggct
      421 accaaagact atctccacca ccttcgccgg cacggccaag accttccagg attcgctggc
      481 caaccagggg ctgctggtcg gcggcgccat tctcaccatc tatatcgtgc tcggcattct
      541 ctatgaaagc ttcatccacc cgctgacgat cctgaccggc ctgccatcgg cggtgctggg
      601 cgcgctgctg gcgctgcggc tggcgggcat ggatttgtcc gtcatcgcca tcatcggcgt
      661 cctcatgctg atcggcatcg tcaagaagaa cggcattatg atgatcgatg tcgccatcca
      721 gttgcggcgc gagggtcgaa cgccggccga agccatccac gaagcctgcc tgatgcgcgc
      781 gcgccccatc atgatgacga cgctcgcagc cctgatgggc accatcccga ttgcgctcgg
      841 caccggggcc agcgccgaat tgcgccagcc gctcggcgtc gcggtggtcg gcggcctgct
      901 ggtctcgcag gcgctgaccc tgttcgtcac gccggtgctt tatctctaca tggaaagctt
      961 ttccggctgg cttttaagcc tgggaccaaa gcgcaagcct gcgccgccgc agattgccga
     1021 gatagaacct ggccacaagc caaggcctga agaggcggcg gttcacaagc cggagcttgt
     1081 ccgcgtcgga tcgtagtcga tccgcgccgg ctgcaagatc atccgaccga ttactgtcgc
     1141 gccgtttctc cccggaagga cctgtgtgcg gctctcattg gcaagttctg ctggtcgaaa
     1201 gaccggcgcg acgggccaag ccattgagcc taaacgggaa agatgccgct gccgtcgggc
     1261 gccggctggc ccgtgcaatt aactatggaa tcaggcattc cttttgtagc cggctcttat
     1321 actttacaga tcgtaaagaa agagggcgaa gacatgcgcc cgcaggtgtt agccgggggt
     1381 gttttgatgt ttggcatgac gaaatccaag accttgaaaa ttgaaacgcc ggatctggcg
     1441 atgctggaca tacccggttt cgaggacgat ctgggcgacg ccctgtatgc ctccacggag
     1501 gatggcgcgc ttcctgacgc agaggatgtc gagcaggatg cgcagccggt ggacggggat
     1561 gttcaggtgg ccgccgccgc gccccggcag tccacggcga cccgcgccaa cctcgccgcg
     1621 ctggccgcct tcgacggcga aatgcgcagc gcctccgctg gtctcgacgc catggaaacc
     1681 accatggcgg cccttggatc ggcccatgag gcggcaaggc gcctgctcgg caatctccat
     1741 gccggtgtca tcagggcgaa cgaccttgaa gaggccaatg ccgcgctcct ggccgacaac
     1801 cgccggctgg ccgaaaggct ggaacagtcg acgcatcttc aaagccagct gcaaagcacc
     1861 gacgaggtca acaggcggcg gatcgagctt ctcgccaggg atcatgagga cgccaagctg
     1921 gcgctcggca agtcccagct tgaggcgatc gagctaaatg ccgcctttgc cgaagccgat
     1981 ggcgaacgaa ccgcgctgac ctacgagctg gccgccaaat cgacggcggc ggaccggctt
     2041 gccagggaaa acgaactgac ccgccagaag ctcgcgaacc agcagatgct gaatacggaa
     2101 ctggagcagc gccacgccga actggagcgc aagctggaag aggtgatttc ggttcgcaag
     2161 gccgagacgg cggaaatgtc ggaactgcgc atgcgcttcg acagcgtaga caaggaatat
     2221 aagcgcctcc agaaacagtc cgagctttcg caggcaaggc tcgttgagac ccaggagcgc
     2281 ctgatgagcc ttgaggccga tctggacgaa ttgggcaacc gccatggcgc ggcggccgag
     2341 cggttcagga ccgaaaccga aacgctcaag acgaagctcg aagccgcaaa caggaaggcc
     2401 ttggccgatg cggaggagat cgcatcgctc aagttggagg ttggcgatgc ggtgtccgcc
     2461 gccaatgtcg tcagcgcgca gcttgcctgc gcaaacggcc agatcgatgc gtttcgctcc
     2521 tcaagcgctg cattgacttt caagcgcacc ggccagacag gcggcgagac gataacgcgc
     2581 ggcccggtgt cgtatgacag ggaccggacg gccaagaaag caaagaagcc attggccgag
     2641 accagccgca cagccaagcc cgagagccgg gtcgtccatc cgaggaaaga gcacggctag
     2701 agcatttcca gcaaaagtgt gaagcggttt tgcgtccgga aatgcggcca aaacaatgga
     2761 gcggttttac cgattcggta acgaccagaa ccgctctaag attataccgc cgctccacgc
     2821 ggcggcttgc ctgcctagat cgggcttttg tcgcgcagtt ctcgccgcag gattttgccg
     2881 accggcgtct tcggcaactc ggtgcggaat tcgacgaagc gcggccgctt gtagccggtc
     2941 aggttcgcgc ggcaataagc gataaggtcc gcttccgtca ggttggggtc tttcctgacc
     3001 acgaacagct tcggcgcttc gcccgaatgc tcgtcgggaa cgccgatggc ggcgacctcg
     3061 agcacgcccg gatgctgggc caccacctct tccagctcat ttggatagac gttgaagccg
     3121 gaaacaagga tcatgtcctt cttccggtcg acgatcttgg tgaagccggc cctgtccatg
     3181 aagccgatat cgcccgactt gaagaaaccg tcagccgtca tcaccttggc cgtctcgtcg
     3241 ggccggttcc agtagccggc catcacctgc ggtcccttga tgcagatttc gccgacctcg
     3301 cccggcgcca gatcgttgcc gtcatcgtcg cggatggcga tctcggtcga tggcatgggc
     3361 agcccgatcg tgccggtgaa atccggtgac gagcatttgt tggctgtcgc caccggcgac
     3421 gtttccgaaa gcccgtagcc ttcggtgatg tgattgccgg tcagttgctt ccagcggtcg
     3481 gccacgccct tctgcacggc catgccgccg ccgaggctga ggcgcaacgc gctgaagtcg
     3541 agcttgcgga attcgtcatt gttcatcagc gcgttgaaca gcgtgttgag gccgggaaaa
     3601 atatggatcg gctgtttctg gagttccttg acgaagcctg gaatgtcgcg cgggttgggg
     3661 atcaggatat tttgcgcgcc ctgctgcatg cccatcagcg cgttcaccgt cagcgcgaag
     3721 atgtgataga gcggcagcgg gcatatatag gtcagatgcg ccggtttcgg cttggccgtg
     3781 taggcatcct ccacccacag cgccaattgc gcgacattgg cgattacgtt gcggtgcagc
     3841 agcgtcgcgc ccttggaaac gccggtggtg ccgccggtat attgcaggaa ggcgatatcg
     3901 tcggcggccg ccttgaccgg cttgaaggcc agcgcctcgc ccgccttcag cgcggcattg
     3961 aactttatat gtccgggcag cgaccacgcc ggcaccagct tcttgacgcg ccgcacgacg
     4021 agattgacga tggcgccctt caccgcgccc aagaggtcgc ccatcgaagc gacgacgaca
     4081 tgcctgatcg ccgtgttgtg aacgactgcc tgcaaggtgg tggcgaagtt ttccaggatg
     4141 acgatcgctt ccgcgcccga atccttcaac tggtgctcaa gctcgcgcgg catatagagc
     4201 ggattgacgt tgaccacgac gtagccggcg cgcaggcagg ccatcatcgc cacgggatat
     4261 tgcagcacgt tcggcatcat cagcgcgacg cgcgcgcctt tcggcatgcc cgtggattga
     4321 agccatgccg cgaaagcgga cgactggcgc tccatctcgg cataggaaag gcttttcccc
     4381 atgcaggtga aggcgggctg gccggcgaac tgcttgcatg ccttcaccag caactcgccg
     4441 atggaatggg tcggcaacgc gccgatctcg gccggcatgt tcttcggata gcttgccagc
     4501 cagggtttct ggagtggctt ctccggagca ggtattttgg cggctccggc aagcgccttt
     4561 ggcacggctt tcacgggggc tttcgcaggt gctttcgcgg cggttttagc aacgccagcg
     4621 gccggctttc ccgcagatgc ggcggtgttg ttcgccgccg gcttttcggg cgcccgcttc
     4681 gcctttgcat tgcggggcgc gcctttggct gcgacaggtt cggccggcgt ggtccttgcc
     4741 gcgtccttcg cccggtcagg cttggccgcg actttctgct tgctccgggt tgccgttcca
     4801 gtcgcctttg ccacacgttc ctcccatgcc tgacatccca tgcctgacat ctatgtattg
     4861 gttactatcg gcgcgcttgc aagcctcgcc catgcgcccg cgccgcgcca cggcaggcga
     4921 ttgccgcgcg cgcaagacct cgcctcgcaa gcgtcttttc agccgcgtcc tgtgcttgcg
     4981 cgggatcgag cgctgcgggt aaatagttcg aaaggcgaaa aaaagcgcgc tgatatatga
     5041 agcatcgccc gaatttagaa tttcatttca gaattagcat tctgtagaaa tccttttcgc
     5101 tttccttggg gaatgcaagc agggtgttcc actgtgtcta aattggtgct tatatgcggc
     5161 cttatccagc ctgctagagg ggcctggaat aatcagggag tgttccatga aaaagaaact
     5221 gacttttgcg gccgcgttgc tggccgcaac cgtcatgggc ggaatcgcca atgcaaagac
     5281 gctggtttac tgtgcggaag ctgcgccgga aggtttcgac cccgcgccct acacggccgg
     5341 ccagaccttc gacgcctcgt cgcgtaccgc ctacaaccgc ctcgtcgagt tcgagcccgg
     5401 caagaccacc atcgcgcccg gtctcgccga aagctggacg atttccgacg acggccttca
     5461 atatacgttc aagcttcgtc cgggcgtcaa gttccagacc accgactatt tcacgccgac
     5521 gcgcgacttc aacgccgacg acgtggtctt ctccttcgac cgccaggtca acaaggacgg
     5581 cccttggttc cagtacattc caggcatcgc ctatcagtat tacaacgacc agttcggcac
     5641 caacatcacc aaggtcgaga aggtcgacga cctgaccgtg aagttcacgc tcaaggaccc
     5701 ggcgatcacc ttcatcccga cgctcggcat ggatttcgct tcgatcattt ccaaggaata
     5761 tgccgacaag ctccaggccg acaagacgcc tgaactgctc aaccagaagc cggtcggcac
     5821 cggtccttac atcttcgtcg actaccagac cgacgcggtc atccgctaca aggccaaccc
     5881 ggactactgg ggcggcaagc agaagatcga cgacctcgtc ttcgccatca ccaccgacgc
     5941 ctccgtgcgc gcgcagaagc tgaaggccgg cgaatgcaac atcgcctcct atccggctcc
     6001 ggccgacatt gccggcttgc aggccgaccc gaacctgaag gtcgacgagc aggaaggcct
     6061 gaatgtcggc gcgctgatgt acaacaccca gcagaagccg ttcgatgacg tgcgggtgcg
     6121 caaggcgctc aatatggcca tcaacaagca ggcgatcatc gattccgtgt tcgagggcgc
     6181 cggccaggta gccgtcaatc cgatcccgcc gaccatgtgg tcctacaaca aggacgtcaa
     6241 ggacgatccg tacgatccgg aagcagccaa gaagctgctc gacgaagccg gcgtcaagga
     6301 cctgaagatg aacgtctggg ccatgccggt gtcgcgcccc tatatgccca atgcgcgccg
     6361 caccgccgaa ctgatccagt cggatttcgc caaggtcggc gtcacggtcg agatcgtcag
     6421 ctacgattgg ggcgaatacc tcaagcgcgc ctcggccgtc gatcatgacg gcgccgtcat
     6481 cgtcggctgg accggcgaca atggcgatcc ggacaacttc ctctcggtgc tgctcagctg
     6541 ctccgcggtc ggttccaaca accgcgccga gtggtgcaac aaggacttcg atgcgctgat
     6601 caacaaggcc aagacggttt ccgaccaggc cgagcgcacc aagctctacg aacaggcgca
     6661 ggtcatcttc aaggaacagg cgccgtgggc gacgcttgcg cactccaagg tgttcatgcc
     6721 catgcagaag accgtcaccg gctttgcaat ggatccgctc ggcatccatc gcttcgacgg
     6781 cgttgacatt gccgaataag caccgctaaa cggggcggcg ccgcaaggcg tcgccccctt
     6841 ttggctcagt ggtatgctcc gttacattct caacaagatc gccctcatca tcccgacctt
     6901 catcgggatc accattctcg cgttcggctt cgtccgcatc ctgcccggcg atccggtgct
     6961 ggtgctggcc ggcgagcgcg gtatttcgcc cgagcgccat gcggagcttt tgcaccagtt
     7021 cggcttcgac ctgcctatct ggcaacaata ctggatttac ctgtccaata tcgcccatgg
     7081 cgatttcggc atttcattca ccaccaagac gccggttctg cgcgacttcc tcgttcgctt
     7141 cccggcgacg ctggaactcg ccttcttcgc catgatcttt gccatcatcc tcggtgttgg
     7201 cctgggcatt ctcgccgccg tgcgccgcgg ttcctggttc gagcagctgt cgatgggaac
     7261 cgccctggtc ggctattcca tgcccatctt ctggtggggc ctgctgctca tcatcctgtt
     7321 ttcgggcatg ttgcactgga cgccggtatc gggccgcatc gaccttttgt acttcttcaa
     7381 accggtcacc ggcttcatga ccgtcgatgc gctgatctcc ggccagaagg gcgcgttccg
     7441 ctccgcggtg tcgcacctca tcttgcccac catcgtgctc ggcaccattc cgctcgccgt
     7501 catcgcccgg cagacgcgct cggccatgct tgaagtgctg ggtgaggatt atgtgcgcac
     7561 cgcgcgcgcc aaggggctgt cgcccagccg cgtcatcggc attcatgcct ttcgcaatgc
     7621 gctcatcccg gtggtcacca ccatcggcct tcagatcggc acgcttatgg ccggcgcgat
     7681 cctgacggaa agcatcttct cctggccggg catcggcaaa tggatgatcg acgccatttc
     7741 caagcgcgac tatttcaccg ttcagggcgg ccttctgctc atcgcgctca gcgtcatggc
     7801 ggtgaacctg atcgtcgacg ttctctatgc cgtcatcaac cctcgcatcc gggtgcagta
     7861 agatgaccaa cgaagccccc gccgccgcgg aagccgccac cctcgtcaag tccgaggacg
     7921 cgcgcctgca aaggttcagc gagttctggt attatttctc catcaacaag ggcgcggtcg
     7981 ccggcctggt cgtcttcgcg ctgctggtgc tgatggcgat cttcgcgccg cttttggcgc
     8041 cccattcgcc tgacgaccag tttcgccaaa gcttcctgac cccgccggcc tggcaggatg
     8101 gcggctcgtg gcagttcgtg ctcggcaccg atgcagtcgg ccgcgacatg ctttcgcgcc
     8161 tgatctacgg cgcgcgtttc tcgctggcga tcggcttcgt ggtggtcatc accgcgctgg
     8221 tttcgggcgt gatcctcggc cttctcgccg gttattgccg tggctgggtc gacacgttca
     8281 tcatgcgcgt catggatatc atcctggcgt tcccctcgct gctgctggcg ctggtgctgg
     8341 tcgccatcct cgggccgggc ctggtcaatg ccatgctcgc catcgccttc gtgttgcagc
     8401 cgcatttcgc ccggctgacc cgcgccgcgg tgatggcgga aaagaaccgc gaatatgtcg
     8461 tctccgccaa ggtggcgggc gccggtcaca tcaggctgat gttttccacc atcctgccca
     8521 attgcctcgc cccgctcatc gtgcaggcga cgctttcctt ctccaacgcc attctggaag
     8581 cgtccgcgct cggcttcctc ggcatgggcg cgcagccgcc gacccccgaa tggggcacga
     8641 tgctggccga ggcgcgcgaa ttcatcctgc gcgcctggtg ggtggtgacc ttccccggcc
     8701 tcgccatcct catcaccgtt ttcgccatca acctgatcgg cgacggcctg cgcgatgccc
     8761 tcgaccccaa gctgaagagg tcgtgatgcg ggtttcagcg aatttctatc ctgacgacgt
     8821 cgcgtacgtt gcgggcgccc agccggtctc cgggcactac cagcctgaag ccgtcggcaa
     8881 tgggttttcc atcgacatcg gtggcgatca tcatcggctt gttgccgaat tcgggcgcga
     8941 tttcgccgac cgagaaatcg atgcggtagc cgtcgcggcc gatgacggcg aacacctggc
     9001 gcaaatcgtc atgatggccg gtttcagcaa tcgcgccatt ggccttgaga acgtcccaca
     9061 gcaacgcacc cttgtagtgt ccgttagaca cgcccttagc ggtctggaag cgggcgtcct
     9121 gctcgatgcc aggcagcttg gcgaggcttg cagccgagat cgaaatcaca gcgcccttgg
     9181 ccgaactgat ttcgatgccc tgggccgccg ccggcccggc ggcagcaagg cccggcgcca
     9241 gggcaacaag caacgccatt ataatcaatc tgtgccccac ggcagcgctc ccatctatgt
     9301 gtgcggcaac cgtaaggcac cgacatatcc gctgcaatcc ttctccagtc gcgcgatgcc
     9361 gctcgacggg gccgctgaac gaaagggccg ccgccatgcc ccttcttgaa atccggaacc
     9421 tcaccgtttc attcgacact tccaccggcc cgttcatggc cgttcagggc atcgaccttt
     9481 ccatcgagcc gcgcgaagtg ctggctatcg tcggcgaaag cggttcgggc aagtcggtgt
     9541 cgatgctggc ggtgatgggg cttttgccga ataccgcgac cgtcaacgcc gatcgcatga
     9601 cgttcgacgg catcgacctc ctgaagatcg acgcatccga gcggcgcaag atcatcggca
     9661 aggatatgtc gatgatcttc caggaaccgg tcgccagcct caatccgtgc ttcaccgtcg
     9721 gcttccagat cgaggaagtg ctgcgctttc atctgggcat gggccgcgcc gaacgccgcg
     9781 cccgcgccat cgaactgatg acgctggtcg gcattccgga cccggccgag cggctgaatt
     9841 cctacccgca ccagatgtcc ggcgggcaga gccagcgcgt catgatcgca atcgccatcg
     9901 cctgcaaccc caagctcttg atcgccgacg aaccgaccac ggcgctggac gtcaccatcc
     9961 agaagcagat tctcgacctc ttggtttcgc tacaggaaaa atacggcatg ggcctgatca
    10021 tgatcaccca caatatgggc gtcgtggccg agaccgccga ccgcgtgatc gtgcagtata
    10081 agggccgcaa gatggaggag gccgacgtgt tgtcgctgtt cgagaacccg aagagcaact
    10141 acacgcgcgc gcttctgtcg gcgctgcccg acaatgccgt cggcgaccgt ctgccgacca
    10201 tatccgacat gctgttcgag ccggctcctt ccgtgggagg cgtcgcatga cccgtgttct
    10261 cgaaggcaag aacattacgc gcgactatca tgtcagcggt ggcctgctgc ggcctgcccg
    10321 caccgtccat gcggtgaagg gcatctcgtt cagcgtcgac aagggcaaga cgctggccat
    10381 cgtcggggaa tcgggctgcg gcaagtccac gctggcgcgc atcatcacgc tgatcgaccc
    10441 ggcgacatcc ggcgagttgc tgatcgacgg ccacaaggtg gatatcgcca aggagagcct
    10501 gtcgaaggaa atgcgccgca aggtgcagat cgtcttccaa aacccctacg ggtcgctcaa
    10561 cccgcgccag aagatcggcg acgttctggc cgagccgctg aagatcaaca ccaacaagcc
    10621 ggctgaagag cggcgcgacc ttgccatggc gatgctcaag aaggtcgggc tggaagaaaa
    10681 gcacttcaac cgctatccgc acatgttctc cggcggccag cgccagcgca tcgccattgc
    10741 gcgcgcgctg atgctgaacc ccagccttct ggtgctggac gagccggtgt cggcgctcga
    10801 tctttcggtg caggcgcagg tgctgaacct gctggccgac ctgcaagacg agttccagtt
    10861 gacctacgtg ttcatcagcc acgacctctc ggtggtgcgc tatatcgccg atgacgtgat
    10921 ggtgatgtat ttcggcgagg cggtcgaata tggctcgcgc gacgaggtct ttgccgatcc
    10981 caagcacagt tataccaagg cgctgtttgc cgcgaccccg cgcgccgatg tcgacagcat
    11041 cagggccagg ctggcgcgca agcaggcggc gtaagctgcc tctgcgccgg agcgtcggga
    11101 cctgacaggt tcggcgcgcg gctatgtcct gccggacttc agcccacatc ccacgaccag
    11161 gcattccaaa gcaggtgcgg cgcgccacat cctgccggcc ttccccagac cgccaggatg
    11221 cggcttccgt cgcgcttgac gctcgccccg ttcgtggaat aaatatttca cgactattcc
    11281 gtttggttct tttgttctga agcccgtccg gctgttttcg gcggcggtcg gggcggggtg
    11341 gccggctgca ttccaatgag ggtctggaca tgacgatacg ttttggcttg ctgggcgccg
    11401 gccgcatcgg caaggttcac gcccgcgcca tcggcgccaa tccgcaggcg aaactggtgg
    11461 cggtggccga cgcgctcgac aaggccgcga aggaaatttc aacggcctac ggcgccgagg
    11521 tgcgcaccat cgacgacatc gagaaagccg ccgatatcga cgccgtggtg atctgcacgc
    11581 cgaccgacac ccatgccgac ctgatcgagc gcttcgccag ggcaggcaag gcgatcttct
    11641 gcgaaaaacc cattcatctg gatgccaagc gcgtcgaaaa atgcctcgcc gtggtgaaaa
    11701 agaccggcgc ggtgctgatg gtcggcttca accgccgctt cgatccgcat ttcgccgccg
    11761 tcaggaaggc catcgacgaa ggcgccatcg gcgcggtcga gatggtgacg atcacctcgc
    11821 gcgatccggg cgcgccgccg gtcgattata tcaagcggtc cggcggcatt ttccgcgaca
    11881 tgaccatcca tgatttcgac atggcgcgct t
//
LOCUS       DASYCS010000063         4714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2622626,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000063 DASYCS010000000
VERSION     DASYCS010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2622626"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /locus_tag="VGM46_02335"
     CDS             <1..979
                     /locus_tag="VGM46_02335"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEY4191459.1"
                     /translation="LMPRYAELIYNGFWFSPERLMLQAMIDKSQEDVEGTVRLKLYKG
                     NDQVRFELSAYALNPDIKVIAPWRDWTFKSRTDLLNFAEQHQIPVPKDKKGDAPFSVD
                     ANLLHSSSEGKVLEDPWSEPPEFVHQRTISPMDAPDEVTEIEIEFLRGDPIALNGEKK
                     SPASMLAALNDLGRDNGIGRLDLVENRFVGMKSRGVYETPGGTILLAAHRGIESITLD
                     RGAGHLKDELMPRYAELIYNGFWFSPERLMLQAMIDKSQEDVEGTVRLKLYKGNVIVT
                     GRKSAKSLYRNDLVTFEDDRGAYDQKDAAGFIRLNALRLRTLAARNRSK"
     gene            complement(1058..1873)
                     /locus_tag="VGM46_02340"
     CDS             complement(1058..1873)
                     /locus_tag="VGM46_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEY4191460.1"
                     /translation="MAFFAKGKIFAAAIAAVFAATAGAEAAGKCGKDSSGFAAWVVAY
                     KADAQSRGIDTGPLAGVRYDTGVIRIDRNQHAAFRGTVDQFLARRAPASYVSKAKGYM
                     KSQAGLLKKIQARYGVQPEVVLAIWGMETGFGANSGNKSVFGPLATLAYDCRRSAYFT
                     NELDSALEIAQKGYISASQMKGAGFGELGQTQFLPSKYLQYAVDFDGDGRRDLIHSRA
                     DVLASTANFLRGHGWSAGGGYGPGETNFGVWNDWNRASVYQKALAEFARRIAN"
     gene            complement(2042..2740)
                     /locus_tag="VGM46_02345"
     CDS             complement(2042..2740)
                     /locus_tag="VGM46_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018430479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2270 domain-containing protein"
                     /protein_id="HEY4191461.1"
                     /translation="MANTDKKIQPSPLPETHSEVVTAGVHYYRAELARMAGWRDRIDR
                     TTNWAITVVAALLSVSLSTASAHHGVLLFAMLLVYLLLMIEARRYRFFDVYRFRVRQF
                     ERHYFAQVFSPAADFSANWTQILGQSLREPKFFISYGDAFSRRLRRNYIWMFLILLLA
                     WILKISTPRLQDEGAGFRLAHSLEEVLTNAGFGPIPGWLVVAGVLLFFAWLLFASLRA
                     PDDAGEGDFGEVHV"
     gene            3074..4663
                     /gene="ffh"
                     /locus_tag="VGM46_02350"
     CDS             3074..4663
                     /gene="ffh"
                     /locus_tag="VGM46_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEY4191462.1"
                     /translation="MFESLQERLGSILNGLTGRGALSEADVTAALREVRRALLEADVA
                     LEVVRSFTDKVREKAVGAAVVKSIKPGQMVVKIVHDELISMLGDEGVAIDLNAPAPVV
                     IMMVGLQGSGKTTTSAKIAKRLTDRQNKKVLMASLDTRRPAAQEQLRQLGEQVKVATL
                     PIVAGQTPVEIARRAVQAAKLGGHDIVILDTAGRTHIDEPLMVEMADIKKVSSPHEIL
                     LVADSLTGQDAVNLAKNFDERVGITGLVLTRMDGDGRGGAALSMRAVTGKPIKLIGTG
                     EKMDALEEFYPKRIADRILGMGDIVSLVEKAAENIDAEQAAAMARKMQSGKFDLNDLA
                     GQLQQMSKMGGMGGLMSMMPGMGKMKDQMAAAGLDDKMFGRQLAIISSMTRAERANPD
                     LLKHSRKKRIAAGSGTDAAEINKLLKMHRGMADMMKAMGGKGKGGGLMRGMMGGLASK
                     MGLGGGMPGMGGMPDLSKMDPKQLEALKKQAEASGFGGGMGKGLPGSLSGGLPGGLPG
                     LPGGIKLPGLGGGLPPRGGKK"
BASE COUNT          916 a         1422 c         1553 g          823 t
ORIGIN      
        1 gttgatgccg cgttatgcgg agctgatcta taacggcttc tggttctcgc cggagcggct
       61 gatgttgcag gcgatgatcg acaagagcca ggaagacgtc gagggcacgg tgcggctgaa
      121 gctctacaag ggcaacgatc aggtccgctt cgagctttcc gcctatgcgt tgaaccccga
      181 catcaaggtc atcgcgccgt ggcgcgactg gacgttcaag tcgcgcaccg atttgctgaa
      241 tttcgccgaa cagcaccaga tcccggtgcc gaaggacaag aagggcgacg cgccgttttc
      301 ggtggacgcc aatctgctgc actcttcctc ggaaggcaaa gtgctggaag acccgtggag
      361 cgagccgccg gaattcgtgc atcagcgcac catttcgccg atggacgcac ccgatgaggt
      421 caccgagatc gagatcgaat ttttgcgcgg cgacccgatt gccctgaatg gcgagaaaaa
      481 atcaccggct tcgatgctgg cggcgctgaa cgatcttggc cgcgacaatg gcattggccg
      541 gctcgacctg gtggaaaacc gcttcgtcgg catgaagagc cgcggcgtct atgaaacgcc
      601 gggcggcacc atcctgctgg cggcgcatcg cggcatcgaa tccatcacgc tcgatcgcgg
      661 cgcggggcat ttgaaggacg agttgatgcc gcgttatgcg gagctgatct ataacggctt
      721 ctggttctcg ccggagcggc tgatgttgca ggcgatgatc gacaagagcc aggaagacgt
      781 cgagggcacg gtgcggctga agctctacaa gggcaacgtc atcgttaccg ggcgcaagtc
      841 ggcgaaatcg ctctaccgca acgacctcgt taccttcgag gacgatcgcg gcgcctacga
      901 ccagaaggac gcggcaggct tcatccgcct caacgcgctg cgtctgagga cgctggccgc
      961 gcgcaaccgg tcgaagtgat gcagcgtcgg ctttgctgac cgactgcaaa taaaaaagcc
     1021 cggcattgcc gggctttttc gtcggtgggt atcggtgtca gttggcgatg cggcgggcga
     1081 attcggccag tgccttctgg tagaccgagg cgcggttcca gtcgttccag acgccgaaat
     1141 tggtttcgcc cggaccgtag ccgccgccgg cgctccagcc atggccgcgc aggaagttgg
     1201 cggtggaggc cagcacgtcg gcgcgcgaat ggatgaggtc gcggcggccg tcgccgtcga
     1261 agtcgacggc atattgcagg tatttggaag gcaggaactg ggtctggccg agttcgccga
     1321 agccggcgcc cttcatctgg ctggccgaga tgtagccttt ctgggcgatt tccagcgccg
     1381 aatccagctc gttggtgaag taggccgagc ggcggcagtc ataggccagc gtcgccagcg
     1441 ggccgaacac gctcttgttg ccggaattgg cgccgaaccc ggtttccatg ccccagatgg
     1501 cgagaacgac ttcaggctgc acgccgtaac gcgcctggat tttcttcagc aggccggctt
     1561 gcgacttcat gtagcccttg gccttgctga cataggaagc gggcgcgcgg cgcgccagga
     1621 actgatcgac cgtgcccctg aaagccgcat gctggttgcg gtcgatgcgg atgacgccgg
     1681 tatcgtaacg cacgccggcc agcggaccgg tgtcgatgcc gcgcgactgg gcgtcggcct
     1741 tgtaggcaac gacccatgcg gcgaagccgc tggaatcctt gccgcatttg ccggcggctt
     1801 cagcgccagc ggtcgccgca aagacggcgg caatcgccgc cgcaaaaatc ttacccttgg
     1861 caaaaaatgc catgcttctc tcctggttgc ctgaatcatg cccctgaagg cgaacttgcc
     1921 agtgaaacgc cggcttgtca ccgtcgtttt cgcgaacccc ggcgcccgcc aatccatggc
     1981 gaacttgccc acgatcaagg gataattgtg gcgaaagcct gatctttatg gcgaagcgcg
     2041 ttcagacatg cacctcgccg aaatcccctt cgccggcatc gtcgggcgca cgcaacgagg
     2101 caaacagcag ccaggcgaag aacaaaagga cgccagccac gaccagccag ccgggaatcg
     2161 gcccgaaacc ggcattggtc agcacttcct ccagcgaatg ggcgagcctg aagccggcgc
     2221 cttcatcctg caatctcggc gtcgatattt tcagtatcca ggccagcagc aggatcagga
     2281 acatccagat gtagtttcgc cgcagccgcc gcgaaaaggc gtcgccgtag gagatgaaga
     2341 atttcggctc gcgcaggctc tggccgagga tttgcgtcca gttggcggaa aagtcggcgg
     2401 cgggcgagaa cacctgcgcg aagtaatgcc gttcgaactg gcgcacccgg aagcgataga
     2461 cgtcgaagaa gcgatatcgg cgcgcctcga tcatcaagag caggtagacc agcagcatgg
     2521 cgaacagcag aacgccgtga tgggccgaag ccgtcgacag cgaaaccgac agcagggcgg
     2581 cgacgaccgt gatcgcccag ttggtggtgc ggtcgatgcg gtcgcgccag ccggccatgc
     2641 gggccagttc ggcgcggtag tagtgaacgc cggcggtcac cacttccgaa tgggtttcgg
     2701 gcaatggcga aggctggatc tttttgtccg tgtttgccat gacgcccacc tccatcgttg
     2761 ctccacctct tcgatatagg gcggctgccg gccactgtcg tacggcattc ttgactcttt
     2821 tgcgtttttc ctgtctacag cccacgaaat tccgcgacga gtttatctcc gagcaagccg
     2881 accgggacgc cgaggccttc gagacagggc caggatcgat cccggaggtc aacacccggc
     2941 agcgctgtgc gcccccgggt gctttttggc tttgggcttt gcccaatggt ttggctgctg
     3001 gttttgcttg gatgcccggc gcgaacgcat tacctctcgg gcatcgtcct gatgcctggg
     3061 aaaagggaaa acaatgttcg agagcttaca agagcgcctt ggctccatcc tgaacggcct
     3121 taccggccgt ggcgcgttgt ccgaagccga tgtgacggcg gcgctgcgcg aggtgcgccg
     3181 cgcgctgctg gaagccgacg tggcgctcga agtggtgcgc tccttcaccg acaaggtgcg
     3241 cgagaaggcc gttggcgcgg cggtcgtcaa gtcgatcaag cccggccaga tggtcgtcaa
     3301 gatcgtccat gacgagctga tttccatgct gggcgacgaa ggcgtcgcca tcgacctcaa
     3361 tgcgccggcc ccggtcgtca tcatgatggt tggcctgcaa ggttccggca agaccaccac
     3421 cagcgccaag atcgccaagc gcctgaccga ccgccagaac aagaaggttc tgatggcgtc
     3481 gctcgatacg cggcgtcccg ccgcgcagga gcagcttcgc cagcttggcg aacaggtgaa
     3541 ggtggcgacg ctgccgatcg tcgccggaca gacgccggtc gagatcgcca ggcgcgccgt
     3601 gcaggcggcc aagctcggcg gccacgacat cgttatcctc gacaccgccg gccgcaccca
     3661 tatcgacgag ccgctgatgg tcgagatggc cgatatcaag aaggtatcgt cgccgcacga
     3721 aatcctgctg gtcgccgatt cgctgaccgg ccaggacgcc gtcaaccttg ccaagaattt
     3781 cgacgagcgc gtcggcatca ccggccttgt cttgacccgc atggacggcg acggccgtgg
     3841 cggcgcggcg ctttcgatgc gcgcggtgac cggcaagccg atcaagctga tcggcaccgg
     3901 cgaaaagatg gatgcgctgg aggagttcta ccccaagcgc atcgccgacc gcatcctcgg
     3961 catgggcgat atcgtctcgc tggtcgagaa ggccgccgaa aacatcgacg ccgaacaggc
     4021 ggcggcgatg gccaggaaga tgcagtcggg caagttcgac ctgaacgacc ttgccggcca
     4081 gttgcagcaa atgtcgaaaa tgggcggcat gggcggcctg atgagcatga tgcccggcat
     4141 gggcaagatg aaagaccaga tggccgccgc cgggctggac gacaagatgt tcggccgtca
     4201 gctggccatc atctcttcga tgaccagggc cgagcgcgcc aatcctgatc tgctgaagca
     4261 ttcgcgcaag aagcgcatcg ctgccggttc cggcaccgat gccgccgaaa tcaacaagct
     4321 gctgaagatg catcgcggca tggccgacat gatgaaggcg atgggcggca agggcaaggg
     4381 cggtggcctg atgcgcggca tgatgggcgg gctggcctcc aagatggggc ttggcggcgg
     4441 gatgcccggc atgggcggga tgcccgatct gtcgaagatg gaccccaagc agctcgaagc
     4501 attgaagaaa caggccgagg cgtcgggatt cggcggcggc atgggcaagg gattgcccgg
     4561 cagtttgtct ggtggcctgc ctggcggatt gccgggtctt cccggcggca tcaagcttcc
     4621 cggactgggt ggcggactgc cgccaagggg cggcaagaaa tgacgccgcg cctcgaaacc
     4681 gaacgtctcg tgctgcgcgc cttcgcgcct ggcg
//
LOCUS       DASYCS010000064        14137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2912200,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000064 DASYCS010000000
VERSION     DASYCS010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 14137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14137
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2912200"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VGM46_02355"
     CDS             complement(<1..308)
                     /locus_tag="VGM46_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019858410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HEY4191463.1"
                     /translation="MKAEETTAPQDSSAMARLRDALRKLYHGNSPLAFKFQLAAAAVD
                     LAIIAFFIATPLLQETRSFLWVDYLVAAIVAADMLARLLASTDMLRLMRQPTWLVDFF
                     "
     gene            complement(366..3848)
                     /gene="dnaE"
                     /locus_tag="VGM46_02360"
     CDS             complement(366..3848)
                     /gene="dnaE"
                     /locus_tag="VGM46_02360"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEY4191464.1"
                     /translation="MLQAARPEPSARPFIHLRVHSAYSLLEGALQLPKVVAHAVKDEA
                     PAIAVTDTNNLFGALEFAQKAVKDGIQPIIGCQVDLAFSGENSDGQRDRRKQGPEMRP
                     VVLIAATQAGYASLVRMVSRVYLETPPGEPVHLSTDMMAGHCEGLICLSGGPRGPIGN
                     AIREDRRDLAETRLLALKGLFGDGLYVELERVGAYDRAIEQVSIDLAYSHELPLVATN
                     EAFFSAREDYEAHDALIAIAEGSVVAADNRRRLSPDNFLKSQAEMAKLFADLPEAIDN
                     TVEIAMRCSYYPKNRNPILPRFTGGEVADADAAVKAEAEELARQAWEGLDARVAIHGV
                     TPGHTVEEYRERLAFELGIIERMKFPGYFLIVADFIKWAKAQGIPVGPGRGSGAGSLV
                     AYALTITDIDPLRFSLLFERFLNPERVSMPDFDIDFCQDRREEVIRYVQGRYGRDQVG
                     QIITFGTLQARAVLRDVGRVLQMPYGQVDRLCKMVPQNPAHPVSLGQAIEGEPRFAEE
                     AEKEPIVQTLLDMAQKLEGLYRHASTHAAGIVIGDRPLSELVPMYRDQRSDMPVTQFN
                     MKYVEQAGLVKFDFLGLKTLTVLNTAVKLIARRGIEIDLAHIPLDDEKTYAMLSRGEV
                     VGVFQVESAGMRKALLGMKPDCIEDIIALVALYRPGPMENIPTYNARKHGEEELASIH
                     PKIDHLVKETQGVIVYQEQVMQIAQELSGYSLGEADLLRRAMGKKIRAEMDKQRERFV
                     SGAVERGVGKPQADFIFDLLAKFADYGFNKSHAAAYAVVSYQTAYLKAHYPVEFLAAS
                     MTLDMGNTDKLADFRQDALRLGIDVVAPSVMTSFRAFEVGENRIFYSLAALKGVGDAA
                     VEHIVEKRKDGPFKSLADFCERIDPKIAGKRVFESLITAGALDCFGYDRASMMAGVER
                     MMGLAALAQQNAISGQHDIFGASLGSQSQALNLPAVDAWLAADRLHREFQIVGFYLSA
                     HPLDEYKAALEKMRVQNWGEFSAAVKRGASAGRLAGTVTSKQERKTRTGNKMGVVTFS
                     DTTGQYEAVLFSEALAQFRDLLEAGRSVVITVTAEDRPEGINLRINGVQSLEEEASRV
                     QTALRIFVRDAAPLGTLAGQLGTKGEGQVSIIVLKDAAQGEVEIELPNRYRISPQVAS
                     AMRAVPGVVEVELV"
     gene            complement(4023..6035)
                     /locus_tag="VGM46_02365"
     CDS             complement(4023..6035)
                     /locus_tag="VGM46_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019858412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HEY4191465.1"
                     /translation="MFSKILIANRGEIACRIIKTARKMGIGTVAVYSDADRDAVHVEM
                     ADEAVHIGPAPAAQSYLLPEKIIAACKATGAEAVHPGYGFLSERAAFCEALEAEGIVF
                     IGPKPKAIRAMGDKIESKKFANAAKVSTVPGYLGVIEDADHAEKIAGEIGYPVMIKAS
                     AGGGGKGMRIAWSEAEVRDGFDRARSEAKNSFGDDRVFIEKFVVDPRHIEIQVLGDAH
                     GNCIYLGERECSIQRRNQKVVEEAPSPFLDAATRKAMGEQAVALSKAVDYQSAGTVEF
                     IVDAQKNFYFLEMNTRLQVEHPVTELITGIDLVEQMIRIAAGEKLSIGQGDVKLNGWA
                     VESRLYAEDPYRNFLPSIGRLTRYRPPEEGKIGDIVIRNDTGVTEGSEISMFYDPMLA
                     KLCTWAPTRLAAIDAMSEALDSFVVDGIEHNIPFLSALMQHPRWREGRLSTAFIAEEY
                     PDGFTPVVANDDEKAVLAAIAVSVELLRRDRLDRLGGRLAPHSGALKREWVVKIGNDY
                     LSAEILDGMISIPMEVDLSIEGGKPLTVASDWRPGDAIWRGTVGGRTVVAQLRTLPNG
                     LRMAWKGMAVSALTMLPRTAALEKLMPEKVAPDTSKLLLCPMPGLVVSIGVAEGQEVK
                     AGDTLAVVEAMKMENVLRAERDLTVSKLNAKAGDSLAVDAVIMEFA"
     gene            6034..6216
                     /locus_tag="VGM46_02370"
     CDS             6034..6216
                     /locus_tag="VGM46_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191466.1"
                     /translation="MLGFVLVSASPCMESRNAAQAGKTTSAPSASRRPENMPQTGFPP
                     PRLPDSLAGIKTFPKI"
     gene            6245..7372
                     /locus_tag="VGM46_02375"
     CDS             6245..7372
                     /locus_tag="VGM46_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007792251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HEY4191467.1"
                     /translation="MKTRAAIAVGAGKPLEIMEVDLEGPREGEVLVEIKATGICHTDE
                     FTLSGADPEGIFPAILGHEGAGVVVDVGKGVTSVKKGDHVIPLYTPECRQCPSCLSRK
                     TNLCTAIRATQGQGLMPDGSSRFSIGKDKIFHYMGCSTFSNFTVLPEIAVAKVNPDAP
                     FDKICYIGCGVTTGVGAVINTAKVEIGATAVVFGLGGIGLNVIQGLRLAGADMIIGVD
                     LNNDKKAWGERFGMTHFVNPKEIDGDIVPYLVNMTKRGADQIGGADYTFDCTGNTKVM
                     RQALESSHRGWGKSVVIGVAGAGQEISTRPFQLVTGRTWMGTAFGGARGRTDVPKIVD
                     WYMDGKIEIDPMITHTLKLEDINKGFDLMHAGESIRSVVVY"
     gene            7461..7928
                     /locus_tag="VGM46_02380"
     CDS             7461..7928
                     /locus_tag="VGM46_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018427761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HEY4191468.1"
                     /translation="MTLPAILVDADACPVKAEIEKVAARHGIAVTFVSNGGLRPSRDP
                     MIRNVVVSKGADAADDWIVENARSNDIVVTADIPLAARAVALGAHVLGPSGRPFTPET
                     IGMAVAMRDLKQHLRETGESKGYNAGFSPQDRSRFLGELDRILRRAINSAKAP"
     gene            7928..8611
                     /locus_tag="VGM46_02385"
     CDS             7928..8611
                     /locus_tag="VGM46_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HEY4191469.1"
                     /translation="MATGKPTEKKLLHRHMQFAISACIGVVVLFIALFFRAPLAYSIG
                     ANAFFVSYIWLVLVQMPSLSASYLSHNARATDQPVAIIFAVTLVVVGVATVSLFLLIN
                     SKENTGLFALIFALASIPLGWFTIHAMTALHYAHIYWMDDAGDGSGSRPARKLPVGGL
                     SFPDTERPQGWDFLYFATVIGMTAQTADTGITTTQMRRIVLLHSVVSFFFNTVIVAAA
                     VNLAVSLGG"
     gene            8718..9590
                     /locus_tag="VGM46_02390"
     CDS             8718..9590
                     /locus_tag="VGM46_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006201605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEY4191470.1"
                     /translation="MESIARAETDIPQTAPGKTGPVIYRHSRWTRLTHWLWAIALFFL
                     LLSGLQIFNARPQLYVGQESGFAYNNTILQIGAENTDAGPRGFTEIFGKRFDTTGVLG
                     WSGPAGQESAKAFPSWATIPSYYDLGTARVVHFFFAWTLSATLLLWLAASLINGHLRR
                     DLAPRADDIRRLPRDIADHARLKFHHVREYNTLQKLAYGGVLFVALPLMVLTGLTMSP
                     SMNTVLPFLPEVFGGRQTARTIHFGIMALLVLFFIVHMLMILAAGPINELRSIITGWY
                     RTDPPSNDHPDERS"
     gene            9592..10383
                     /locus_tag="VGM46_02395"
     CDS             9592..10383
                     /locus_tag="VGM46_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEY4191471.1"
                     /translation="MAKFQLNRRKFLTASSLGLSGLVLSGCDAFDSQLGLGDGLRSFL
                     EGANDLTHATQRLLTGRDSLAHEFTEADIRQPMRPNGVTAPEDETYKSLLANDFADWR
                     LEVSGLVEKPLSLSRDQLTAMPSRTQITRHDCVEGWSCIAKWTGTPLALVLDQAVVKP
                     QARYVLFHCLDTIDRDLSGDIKYYESIDLVDARHPQTILAYGVNGKPLPVENGAPLRL
                     RVERQLGYKMPKYVHKIELVDGFANFGGGRGGYWEDNGYDWYGGI"
     gene            10465..10953
                     /locus_tag="VGM46_02400"
     CDS             10465..10953
                     /locus_tag="VGM46_02400"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY4191472.1"
                     /translation="MVAGALGHVCRAGSIKPRKPRQPSCNHQKEVYRVSDTTNIFEQI
                     PDNDTFGGRFSRARDAIGLSTRELAWRLGVKTATINAWETDRSQPGSRRLALLSGLLG
                     VSLSWLLHGVGLGPMETETAESDEAFGNRLARLKTLHLETGQLILQIQGDLDRLSAVP
                     AR"
     regulatory      11063..11149
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            11302..12402
                     /gene="gcvT"
                     /locus_tag="VGM46_02405"
     CDS             11302..12402
                     /gene="gcvT"
                     /locus_tag="VGM46_02405"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006201608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEY4191473.1"
                     /translation="MTDDQTKHLPLEDLHKAAGAKFGAFAGWSMPLTYPAGVMKEHLH
                     TREQAGLFDISHMKLFEIGGADAVAFLNRACPLDVAALAISQSKYTLLLDEQAGILDD
                     MIVTRLGEQRFMVVANAGNATADEAHLRAVAADFDVRINPLDRVFLAVQGPQAQAALS
                     RAGVETGSLLFMHGYEPRKQWFLTRSGYTGEDGFEIGLPEADARMLLSRLLDDERVQW
                     VGLAARDSLRLEAGLCLHGQDITPGNNPADAGLIWAIPKEIRASGNFIGAAALRNAIA
                     RGPVQKRVGLKPDGRQPVRAGTQLFDAAGNLAGHVTSGGFGPSAGHPVAMGYVTASLA
                     APGSKLFADVRGTRIPTDIHPLPFTPHRYHKG"
     gene            12408..12776
                     /gene="gcvH"
                     /locus_tag="VGM46_02410"
     CDS             12408..12776
                     /gene="gcvH"
                     /locus_tag="VGM46_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006335019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEY4191474.1"
                     /translation="MAATYFTEDHEWLRVEGGVATIGITGYAQEQLGDLVFVELPEVG
                     KKLSKGDTAVVVESVKAASDVYAPVDGEITEANEALSSDPALVNSAPTGEGWLWKMKL
                     SNESQLEGLMDEAAYAKHIG"
     gene            12794..>14137
                     /locus_tag="VGM46_02415"
     CDS             12794..>14137
                     /locus_tag="VGM46_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015317601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase"
                     /protein_id="HEY4191475.1"
                     /translation="MTTAPHFSTRHIGPGLADTRAMLATLGVPSVETLISQAVPKSIR
                     LDRPLDLPAPAGEAEALAELAAVMSRNTVLKSFIGAGYHGVEVPPVIQRNLFENPAWY
                     TAYTPYQAEISQGRLELLFHFQTLVAELTGLPVASASLLDEATAVAEAVGIAVRHHRD
                     KRSKIALAGTPHPQTLDVLHTRAEPLGIKIDGETIDENTAALLVSWPDTFGVYGDHKA
                     AIEKARASGALVIFIADPLGLTLTDAPAKLGADIAVGPMQRFGVPMGFGGPHAAYCAV
                     SDKLTRLMPGRLVGQSNDSKGRPGYRLALQTREQQIRRDKATSNICTAQALLANMATA
                     YAIWHGPAGLQAIAARIHAMAGRLADGLDAAGLKLIGSRRFDTVTVETKGKAGEIAAA
                     AEKSGRLLRIVDADHIGIAFDETSTEADLQAIAALFGARIANDTTGKMPGKPREKE"
BASE COUNT         2658 a         4701 c         4185 g         2593 t
ORIGIN      
        1 atgaagaagt cgaccagcca tgtcggctgc ctcatcagcc gaagcatgtc ggtcgaggca
       61 agaagccggg ccagcatgtc cgccgccacg attgccgcca ccagataatc gacccagagg
      121 aacgaccgcg tttcctgaag cagcggcgtc gcgatgaaaa aggcgatgat ggccagatcg
      181 acggccgcgg cggcaagctg gaacttgaac gccagcggac tgttgccgtg atagagcttg
      241 cgcagcgcat cccgcagccg cgccatggcc gaactgtcct gcggagccgt cgtttcttca
      301 gccttcatgt caccgcgata gcgcccaccg cgtctgccgt ccagtgcagc cggggcgcgg
      361 cgcgctcaaa ccagttccac ttccaccacg cccggcaccg cccgcatggc cgacgccacc
      421 tgcggcgaga tgcggtaacg gttgggcagt tcgatctcga cctcgccctg cgcggcgtcc
      481 ttcagcacga taatgctgac ctgtccctcg cctttcgtgc ccagctggcc ggcaagcgtg
      541 ccaagcggcg cggcatcgcg cacgaagatg cgcagcgcgg tttgcacgcg gctcgcttcc
      601 tcttccagtg actgcacgcc attgatgcgc agattgatgc cctcgggccg atcctccgcc
      661 gtaaccgtga tcaccacaga gcggcccgct tccaacaggt cacgaaactg cgccagagcc
      721 tcggagaaga gcaccgcctc atactggccg gtcgtgtcgg aaaaggtcac gacgcccatc
      781 ttgttgccgg tgcgcgtctt gcgctcctgc ttcgatgtaa ccgtcccggc cagccgtccg
      841 gccgaagcgc cacgtttcac ggcggcggaa aattcgcccc agttctgcac ccgcattttt
      901 tccagcgccg ccttatactc gtcgagcgga tgcgccgaca ggtaaaagcc gacgatctgg
      961 aattcgcggt gcagcctgtc ggcggcaagc cacgcatcca ccgccggcag gttgagcgcc
     1021 tgcgactggc tgccgagcga cgcgccgaaa atgtcgtgct gcccggaaat ggcgttctgc
     1081 tgggcaagtg ctgcaagccc catcatccgc tcgacccccg ccatcatcga ggcgcggtcg
     1141 tagccgaagc aatccagcgc gccggcggta atcaggcttt cgaagacgcg cttgccggcg
     1201 atcttcggat cgatccgctc gcagaaatcg gcaaggctct tgaacggccc gtccttgcgc
     1261 ttttccacga tatgctcgac cgccgcgtcg ccgacgcctt tcagcgcggc cagcgaatag
     1321 aagatgcggt tttcgccaac ctcgaaggcg cggaagctgg tcatcaccga gggcgccacc
     1381 acgtcgatgc ctagccgcag cgcatcctgc cggaaatcgg ccagcttgtc ggtattgccc
     1441 atgtccagcg tcatcgacgc ggccaggaac tcgaccggat aatgcgcctt gaggtaggcc
     1501 gtctggtagg aaacgaccgc ataggcggcg gcgtgcgatt tgttgaagcc gtaatcggcg
     1561 aacttcgcca gaaggtcgaa gatgaagtcg gcctgcggct tgccgacgcc gcgctccacc
     1621 gcccccgaaa cgaagcgttc gcgctgcttg tccatctcgg cgcggatctt cttgcccatg
     1681 gcgcggcgca gcaagtctgc ctcgcccagc gaatagccgg aaagctcctg cgcgatctgc
     1741 atcacctgtt cctgatagac gatgacgccc tgtgtctcct tcaccagatg gtcgatcttg
     1801 gggtggatcg aagccagttc ctcttcgcca tgctttctgg cgttgtaggt cggaatattc
     1861 tccatcgggc cgggccggta gagcgccacc agcgcgatga tgtcttcgat gcagtccggc
     1921 ttcatgccga gcagcgcctt gcgcatgccc gcactttcca cctggaacac gcccaccacc
     1981 tcgccccgcg acagcatggc gtaggtcttc tcgtcatcca gcggaatatg cgccaggtcg
     2041 atctcgatgc cccgccgcgc gatcagcttg acggcggtgt tcagcacggt cagcgttttc
     2101 aggccgagga agtcgaactt caccagtccg gcctgctcga catatttcat gttgaactgg
     2161 gtgaccggca tgtccgagcg ctggtcgcgg tacatcggca ccagttcgga aagcggcctg
     2221 tcgccgatga cgatgcccgc cgcatgcgtc gaggcatggc ggtaaagccc ttccagcttt
     2281 tgcgccatgt cgagcagcgt ctgcacgatc ggttcttttt cggcttcttc ggcaaagcgc
     2341 ggctcgccct cgatggcttg gccgagcgac acgggatgcg ccgggttctg cggcaccatc
     2401 ttgcacagcc gatcgacctg gccatagggc atttggagta cgcgacctac gtcgcggagg
     2461 accgcgcgtg cttgcagcgt tccgaaagtg atgatctggc cgacctgatc gcgtccatag
     2521 cggccctgca cgtagcggat cacctcttca cgccggtcct ggcagaaatc gatgtcgaag
     2581 tcgggcatcg aaacgcgctc ggggttgagg aagcgctcga acagcagcga gaaacgcagc
     2641 ggatcgatat cggtgatggt cagcgcataa gcgaccagcg atcctgcgcc cgaaccgcgc
     2701 cccggcccca ccggaatgcc ctgcgccttc gcccatttga tgaagtccgc cacgatcagg
     2761 aagtagccgg ggaacttcat ccgctcgatg atgccgagtt cgaaggccag cctctcgcgg
     2821 tattcctcga cggtatggcc gggcgtcact ccgtgaattg ccacacgcgc gtcaagccct
     2881 tcccatgcct ggcgggcaag ctcctccgcc tccgccttga cggcggcatc ggcatcggcg
     2941 acctcgccac cggtaaagcg cggcaggatc ggattgcggt ttttcggata atacgagcag
     3001 cgcatcgcaa tctcgaccgt attgtcgatg gcttccggca aatcggcgaa taatttcgcc
     3061 atttccgcct ggcttttgag gaaattgtcc ggcgacaggc ggcggcgatt gtcggcggca
     3121 accaccgagc cttcggcaat cgcaatcagc gcgtcatgcg cctcgtaatc ctcgcgcgcg
     3181 gagaaaaacg cttcgttggt ggcgaccagc ggcagttcgt ggctgtaggc gagatcgatg
     3241 gaaacctgct cgatggcgcg gtcataggcg ccgacgcgct ccagttcgac atatagcccg
     3301 tcgccgaaca gtcctttcag ggcaagaagc cgcgtttccg ccagatcgcg gcgatcctcg
     3361 cggatggcgt tgccgatcgg cccgcgcggt ccgccgctca ggcagatcag cccctcgcaa
     3421 tggccagcca tcatgtcggt gctcagatga accggctcgc cgggcggcgt ttccagatag
     3481 acgcggctga ccatgcgcac gaggctggcg tagcctgcct gcgtcgccgc tatcaaaacg
     3541 accggccgca tctccggccc ttgcttgcgc cggtcacgct ggccatcgct gttctcgcct
     3601 gaaaaggcga gatcgacctg gcagccgatg atcggctgga tgccgtcctt gaccgccttc
     3661 tgcgcgaatt ccagtgcgcc gaacaggtta ttcgtatcgg tgacggcaat cgccggcgct
     3721 tcgtccttca ccgcatgcgc gacgaccttc ggcagttgca gcgcgccttc cagcagcgaa
     3781 taggccgaat ggacgcgcaa atggatgaac ggccgcgcgg aaggctctgg ccttgccgcc
     3841 tgcaacatcg cctcgcgcct cagcgggtca cgaggaagtc tgtcgtctgc catggtttcg
     3901 cgccgctcaa caaagactca taagataggt gtggcggcca ttgtctgaaa cggcggcgcg
     3961 cccgtccagt aaaatggacc ggcagtcata tttggctgtg cgttacgcgc aggcggcagg
     4021 cctcaggcaa attccatgat gaccgcatcg accgccaggc tgtcgccggc tttggcgttc
     4081 agcttggaca cggtcagatc gcgttcggcg cgcagcacgt tttccatctt catcgcctcg
     4141 accacggcaa gcgtgtcccc cgccttgacc tcctggcctt cggcaacgcc aatcgagacc
     4201 accagccccg gcatcgggca caaaagcagc ttcgacgtat cgggcgcgac cttttccggc
     4261 atcagctttt cgagcgcggc ggtgcgcggc agcatggtca gggcggaaac cgccatgccc
     4321 ttccacgcca tgcgaaggcc gtttggcagc gtccgcaatt gcgccaccac cgtgcgcccg
     4381 ccgaccgtgc cgcgccagat cgcatcgccc ggccgccaat cggatgcgac ggtcaacggc
     4441 ttgccgccct cgatcgacag gtcgacttcc atcgggatcg aaatcatgcc gtcgaggatt
     4501 tcggccgaca ggtaatcatt gccgatcttc accacccact cgcgtttcaa cgcgcccgaa
     4561 tgcggcgcga gccgcccgcc aagacgatcc agcctgtcgc gccgcagcag ttcgaccgag
     4621 accgcgattg cggcaagaac cgccttctcg tcgtcattgg caacgacagg cgtaaacccg
     4681 tctggatatt cctcggcgat gaaggcggtc gaaagccgcc cttcgcgcca gcgcggatgc
     4741 tgcatcagcg ccgaaaggaa cgggatatta tgttcgatgc cgtccaccac gaagctgtcg
     4801 agcgcctccg acatggcgtc aatggcggca agccgcgtcg gcgcccaggt gcatagcttg
     4861 gccagcatcg gatcgtagaa catcgagatt tccgatcctt ccgtcacgcc ggtatcgttg
     4921 cgaatgacga tatcgccgat ttttccctct tccggcgggc ggtaacgcgt cagccggccg
     4981 atcgacggca ggaagttgcg atatggatcc tcggcataga gccggctttc caccgcccag
     5041 ccgttcagct tgacatcgcc ttggcctatg gaaagttttt caccggcggc gatacggatc
     5101 atctgctcga ccaggtcgat gcctgtgatg agttccgtca ccggatgctc gacctgcaaa
     5161 cgcgtattca tttcaaggaa atagaagttt ttctgcgcat cgacgatgaa ttcgacggtg
     5221 ccggcgctct ggtaatcgac ggccttggac agcgccaccg cctgctcgcc cattgccttt
     5281 cgcgtcgccg cgtcgagaaa cggcgacggc gcttcctcga cgaccttctg gttgcggcgc
     5341 tggatcgagc attcgcgctc gcccagatag atgcaattgc catgggcgtc gcccagcacc
     5401 tggatttcga tatggcgcgg atcgaccacg aatttctcga tgaagacgcg gtcgtcgcca
     5461 aacgagttct tcgcttccga gcgcgctcgg tcaaagccgt cacgcacttc cgcttcgctc
     5521 caggcaatgc gcatgccctt gccgccgccg ccggcggaag ccttgatcat caccggatag
     5581 ccgatttcac cggcgatctt ctcggcatgg tcggcatctt cgatgacgcc gagatatccc
     5641 ggaaccgtcg ataccttggc ggcattggcg aacttcttgg attcgatctt gtcgcccatc
     5701 gcacggatgg ccttcggctt cggcccgatg aagacgatgc cctcggcttc cagcgcctcg
     5761 cagaaggcgg cccgttccga gagaaatccg tagccggggt ggacggcctc ggctccggtc
     5821 gccttgcagg cggcaatgat tttctcgggc agcagataac tctgcgccgc cggcgccggc
     5881 ccgatatgca ccgcctcgtc ggccatctcc acatgcaccg catcgcgatc cgcatcggag
     5941 tagaccgcga cggtcccgat gcccatcttg cgggcggtct tgatgatgcg gcaggcgatt
     6001 tcaccgcgat tggcgatcag aatcttggaa aacatgctgg gctttgtcct tgtaagcgcc
     6061 agtccttgta tggaatcccg caacgctgct caagcaggca aaacaacatc tgcaccgtcc
     6121 gcgtcgcggc ggccggaaaa catgccgcaa accggctttc cgccgccgcg cttgcctgat
     6181 agcctcgccg ggataaaaac ctttcccaaa atttgatcat cgaatcgcct ggagaacctg
     6241 ctctatgaaa acacgagccg ccatcgccgt cggggctgga aagccgcttg agatcatgga
     6301 ggtcgatctt gaaggcccgc gcgaaggcga ggtgctggtc gaaatcaagg cgacgggcat
     6361 ctgccacacc gacgaattca ctttgtcggg cgccgatccg gagggcattt ttccagccat
     6421 cctcggccat gaaggcgcgg gcgtggtggt cgatgtcggc aagggcgtaa ccagcgtcaa
     6481 gaaaggcgat cacgtcattc cgctctatac gccggaatgc cgccagtgcc cctcctgcct
     6541 gtcgcgcaag accaatcttt gcaccgccat tcgcgccacg cagggccagg gcctgatgcc
     6601 cgacggttcc agccgcttct ccatcggcaa ggacaagatt ttccactaca tgggctgttc
     6661 gaccttctcg aacttcaccg tcctgcccga gatcgcggtt gccaaggtca atccggacgc
     6721 gcccttcgac aagatttgct acatcggctg cggcgtaacc accggcgtcg gcgcggtcat
     6781 caacacggcc aaggtcgaga tcggtgcgac ggcggtggtg ttcggacttg gcggcatcgg
     6841 cctcaacgtc atccaggggc ttaggctggc gggcgccgac atgatcatcg gcgtcgattt
     6901 gaacaatgac aagaaagcct ggggcgagcg cttcggcatg acgcatttcg tcaatccgaa
     6961 ggaaatcgac ggcgatatcg ttccttactt ggtcaacatg accaagcgcg gcgcggacca
     7021 gatcggcggc gccgactata ctttcgactg caccggcaac accaaggtca tgcgccaggc
     7081 gctcgaatcg tcccatcgcg gctggggcaa gtcggtcgtc atcggcgtgg cgggtgccgg
     7141 ccaggaaatc tcgacgcggc cgttccagct ggtcaccggg cgcacatgga tgggcaccgc
     7201 cttcggcggc gcgcgtggcc gcaccgacgt gccgaaaatc gtcgactggt acatggacgg
     7261 caagatcgag atcgacccga tgatcaccca cacgctcaag ctggaagaca tcaacaaggg
     7321 ctttgacctg atgcatgccg gagagagcat ccgcagcgtc gtggtatact gaacggcgat
     7381 cccgcgacct gggccacgaa gaagcccagg cgccgcctgc ctgaatgaat gcaacgccgg
     7441 ctcccgcttc ggaaacaccg atgaccctgc ccgcaatcct cgtcgatgcc gatgcctgtc
     7501 cggtcaaagc ggaaatcgag aaggtggccg cgcgccatgg catcgccgtc accttcgttt
     7561 ccaatggcgg cctgcgccca tcgcgcgatc cgatgatccg caatgtcgtc gtatccaagg
     7621 gcgccgacgc ggctgacgac tggatcgtcg aaaacgccag gtccaacgac atcgtggtga
     7681 cggcggatat cccgctggcg gcgcgcgccg tggcgctcgg cgcgcatgtg ttgggaccga
     7741 gcggccgtcc gttcacgccg gaaaccatcg gcatggccgt ggcgatgcgc gatctcaagc
     7801 agcatttgcg cgaaaccggc gaaagcaagg gctacaacgc cggcttttcg ccccaggacc
     7861 ggtcgcgatt ccttggcgaa ctcgaccgca tcttgcggcg cgccatcaat tccgccaaag
     7921 ctccatgatg gccaccggca aaccgaccga aaagaaactg ctgcaccgcc acatgcagtt
     7981 cgccatatcg gcctgcatcg gcgtcgtcgt gctttttatc gcgctgtttt ttcgcgcgcc
     8041 gctcgcctat tccatcggcg caaacgcgtt ctttgtatct tatatctggc ttgtgctggt
     8101 gcagatgcca tcgctcagcg ccagctatct cagccacaat gcgcgcgcca ccgaccagcc
     8161 ggtggcaatc atcttcgcgg tgacgctggt ggttgtcggc gtcgccaccg tgtcgttgtt
     8221 cctgctgatc aacagcaagg aaaatacggg cctgtttgcc ttgatatttg cgctggcctc
     8281 cattccgctc ggctggttca ccatccatgc catgaccgcc ctacattacg cccatatcta
     8341 ctggatggat gacgccggcg acggctcggg cagcaggccg gccaggaagc tgccggttgg
     8401 cggcttgagc tttcccgaca cggaacggcc gcaaggctgg gacttcctgt acttcgcaac
     8461 cgtgatcggc atgaccgcgc agaccgccga caccggcatc acgaccaccc agatgcggcg
     8521 gatcgtgctt ctccattcgg tcgtgtcgtt tttcttcaat acggtgatcg tcgccgccgc
     8581 cgtgaacctt gcagtcagcc tgggcggctg acctgccgat cacgccgtcg caaaaattcg
     8641 tgagcgcgat gaaacatcca agccgcggcc accgtactgc ggtaagaggc acctgacgac
     8701 acgatcagcg agaagacatg gaatccattg cccgagccga aaccgacata ccgcagaccg
     8761 cccccggcaa gaccggaccg gtgatctatc ggcactcgcg ctggacaagg ctgacccatt
     8821 ggctttgggc gatcgcactg tttttcctgc tgcttagcgg gctacagata ttcaacgccc
     8881 ggccacagct ctatgtcggg caggaatccg gcttcgctta caacaacacc atcctccaga
     8941 tcggcgcgga aaataccgat gccggcccgc gcggctttac ggaaatcttc ggcaagcgct
     9001 tcgacaccac cggcgtgctt ggctggtcgg gcccggcagg ccaggaaagc gccaaggctt
     9061 tcccgtcctg ggcaaccatc ccctcctatt acgacctcgg aacggctcgc gtcgtgcatt
     9121 tcttctttgc ctggacattg agcgcgacgt tgctgctatg gctggcggcc agcctgatca
     9181 acggccatct gcgccgcgac ctcgccccgc gcgccgacga catacgccgg ctgccgcgcg
     9241 atatcgccga ccacgcccgg ctgaaatttc accatgtccg cgaatacaac acgctacaga
     9301 aactggccta tggcggcgtg ctgtttgtgg ctttgccgct gatggtcctg accgggctga
     9361 ccatgtcgcc cagcatgaac accgtgctgc cgttcctgcc ggaagttttc ggtggcaggc
     9421 aaaccgcgcg caccatccat ttcggtatca tggcgctgct ggtgctgttc ttcatcgtcc
     9481 acatgctgat gatcctcgcc gccggcccga tcaacgaact gcggtccatc ataactggct
     9541 ggtatcgcac cgatccgccg tcgaacgacc atccggacga aaggtcctga aatggcgaag
     9601 ttccagctca accgcaggaa attcctcacc gcctccagcc ttggcctgtc gggcctggtg
     9661 ctttcgggct gcgatgcctt cgacagccag ctcggccttg gcgatggcct gcgcagtttt
     9721 ctcgaaggcg ccaacgacct gacccatgcg acgcagcgcc tgctcaccgg gcgcgattcg
     9781 ctggcgcatg aattcaccga ggccgacatt cgccagccga tgcggccgaa cggcgtcacc
     9841 gcacccgagg atgagacgta taagagcctg ctcgccaatg actttgccga ctggcggctg
     9901 gaagtttcgg ggctggtcga aaagccgctt tcgctcagcc gcgatcagct taccgccatg
     9961 cctagccgca cccagatcac ccggcacgac tgcgtcgaag gctggagttg catcgccaaa
    10021 tggaccggca cgccgctggc gctggttctg gaccaggccg tggtcaagcc gcaggcccgc
    10081 tacgtcctgt tccattgcct cgatacgatc gaccgcgatt tgtccggcga catcaaatat
    10141 tacgagagca tcgacctcgt cgacgcccgc cacccgcaga cgatcctcgc ctatggtgtg
    10201 aacggcaagc cgttgccggt ggaaaacggc gccccactgc gcttgcgcgt cgagcggcag
    10261 cttggctaca agatgccgaa atatgtccac aagatcgagc ttgtcgatgg cttcgccaat
    10321 ttcggcggcg gacgcggcgg ctactgggaa gataatggct acgactggta cggcggcatc
    10381 tgagcgcgcc gggacagact tccgattggc gatttacttc cttttgattt caatggcttg
    10441 agataaatcc cgcacctgaa ccttgtggtt gcgggcgccc tcggccatgt ttgccgggcc
    10501 ggatcgatca aaccgcgcaa gccccggcaa ccatcatgca accatcaaaa ggaggtgtat
    10561 cgggtgtccg acaccacgaa tatttttgaa caaatccccg acaacgacac gttcggcgga
    10621 cgtttttctc gagcccgcga cgccatcggc ctcagtacca gggaactggc ctggcggctc
    10681 ggcgtaaaga cggcgaccat caatgcctgg gagacggacc gctcccaacc tggctcgcgt
    10741 cgtttggcgc tgctgtcggg ccttctcggc gtcagcctgt cctggcttct gcatggcgtc
    10801 ggcctcggcc cgatggagac cgagacggcc gaatcggatg aggctttcgg caatcggctg
    10861 gccaggctga agacccttca tctcgaaacc gggcagctca tcctccagat tcaaggcgat
    10921 ctcgaccggc tatccgctgt accggcccgc tgacaggcct gaaatccgaa cagccacaaa
    10981 aaatcgcggc ggcaatgtcg cgcctaggtg acaaagagtc gccgctgggc taaagacagc
    11041 acgtcgaggg ttttcacgaa ccctgccgtt tgcgggagag agcagcgaaa gctgccgccg
    11101 aaggggaaat cgcccgaaat ctctcaggca aaagaaccgt agccggtaag acactctgga
    11161 aagtcggggc gtaaatgtcc cgcgccgaag gtgtaagcgc agccggaaaa gcttccggca
    11221 gcgccagtct ctcaggcttc agacagaggg gcacggacag gtcgccagtt gcggccggct
    11281 gcgtgctttt ctggagaagt catgacggac gaccaaacca aacacctccc cctggaagac
    11341 ctgcacaagg ccgccggcgc gaaattcggc gcctttgccg gctggtccat gccgctgacc
    11401 tatccggcag gcgtgatgaa ggaacacctt catacccgcg aacaggctgg gctgttcgac
    11461 atttcccaca tgaaactgtt cgagatcggc ggggccgatg cggtggcctt tctcaaccgc
    11521 gcctgtccgc tcgacgtggc cgcgcttgcc atttcccagt cgaaatacac actgcttctc
    11581 gacgaacagg ccggcattct ggacgacatg atcgtcacgc ggcttggcga acagcgtttc
    11641 atggtcgtcg ccaatgccgg caacgccacc gccgacgagg cgcatctgcg cgcagtcgcc
    11701 gctgatttcg atgtgcggat caacccgctc gaccgggtat tccttgcggt ccaggggcct
    11761 caggcgcagg cggcactttc ccgcgccggc gtcgaaaccg gttcgctgct gttcatgcac
    11821 ggatatgagc cgcgcaagca atggttcctg acccgctccg gctataccgg cgaggacggc
    11881 ttcgagatcg gcctgccgga ggccgacgcg cgcatgctgc tttcaaggct gctcgatgac
    11941 gaacgcgttc aatgggtggg ccttgccgcg cgcgatagtt tgcggctgga agccggcctt
    12001 tgcctgcatg gccaggacat cacgcccggc aataatccag ccgatgccgg cctgatatgg
    12061 gccattccca aggaaatccg cgctagcggc aatttcatcg gcgctgccgc cctgcgcaat
    12121 gcgatcgcac gcggcccggt tcaaaaacgc gtcggcctga agccggatgg ccgccagcct
    12181 gtgcgcgcgg gcacgcagct tttcgacgcc gcaggcaacc tcgccggcca cgtcacctcc
    12241 ggcggcttcg gcccatcggc aggccatccc gtcgccatgg gctacgtcac cgcatcgctg
    12301 gccgcccccg gttcaaaact gtttgccgac gtgcgcggca caagaattcc aaccgatatc
    12361 caccccctgc ccttcacgcc ccatcgctac cacaaaggat gatttccatg gctgcaacct
    12421 atttcacgga agaccacgaa tggctgcgcg tcgaaggcgg cgtcgccacc atcggcatca
    12481 ccggctacgc gcaggaacag cttggcgacc tcgtcttcgt cgaactgccg gaggtcggca
    12541 agaagctgtc caagggcgac accgccgtgg tggtcgaatc ggtcaaggcg gcgtcggatg
    12601 tctatgcccc cgtcgatggc gagatcaccg aagcaaacga ggcgctgtcg tccgaccccg
    12661 cgctggtcaa ttccgcgcct actggcgaag gctggctttg gaagatgaaa ctttcaaacg
    12721 aaagccagct cgaaggcctc atggacgagg ccgcctacgc aaagcatatc ggctgattgt
    12781 caggacacga acaatgacga ccgctcccca tttttccacc cgccatatcg gccccggcct
    12841 ggccgataca cgcgccatgc tggccacgct cggcgtcccc tccgtggaaa cgctgatatc
    12901 gcaggccgtg ccgaaatcga tccggctcga tcgcccgctc gacctgccgg ccccggcagg
    12961 cgaagccgag gcgctggccg aactggcggc cgtcatgagc cggaacacgg tactgaaaag
    13021 cttcatcggc gccggctatc atggcgttga agtccccccg gtcattcagc gcaacctgtt
    13081 tgaaaaccca gcctggtaca ccgcctatac gccctatcag gccgagatca gccagggcag
    13141 gctggaattg ctgttccatt tccagacgct ggtggcggaa ctgacgggcc tgcccgtcgc
    13201 ttccgcctcc ctgctggacg aagcgaccgc cgtggccgag gccgtcggca tcgccgtgcg
    13261 ccaccaccgc gacaagcgtt cgaagatcgc gcttgccggc acgccgcatc cgcagacact
    13321 cgatgtattg cacacccgcg ctgaaccgct cggcatcaag atcgatggcg aaaccatcga
    13381 cgagaacacc gccgccctgc tggtgtcctg gccggacacg tttggcgtct acggcgacca
    13441 taaggcggca atcgaaaagg cccgcgcttc cggggcgctc gtcatcttca ttgccgatcc
    13501 gcttggcctg accctgaccg acgcgccggc aaagcttggc gccgacatcg ccgttggccc
    13561 gatgcagcgc ttcggcgtgc cgatgggttt tggcgggccg cacgccgcct attgcgccgt
    13621 ttccgacaag cttacccggc tgatgccggg ccgcctcgtc ggccaatcca atgacagcaa
    13681 gggccgtccc ggctaccgcc tcgccctgca aacacgcgaa caacagatcc gccgcgacaa
    13741 ggcgacctcc aacatctgca ccgcgcaggc gctgcttgcc aacatggcca cggcctacgc
    13801 aatctggcac ggcccggccg gcttgcaggc gattgccgcg cgcatccacg cgatggccgg
    13861 ccgccttgcc gacggcctgg acgccgccgg cctgaagctt atcggctcgc gccgcttcga
    13921 cacggtgacg gtcgagacca aaggcaaggc cggggaaatc gccgccgccg ccgaaaaatc
    13981 cggccgcttg ctgcgcatcg tcgatgccga ccacatcggc atcgccttcg atgaaacctc
    14041 aaccgaggcc gaccttcagg cgatcgccgc gctgttcggc gccaggatcg ccaatgacac
    14101 caccggcaaa atgccaggaa aaccgcgcga aaaagaa
//
LOCUS       DASYCS010000065         4089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_303807,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000065 DASYCS010000000
VERSION     DASYCS010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4089
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303807"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="VGM46_02420"
     CDS             <1..624
                     /locus_tag="VGM46_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191476.1"
                     /translation="LPFFSGIGLLFIVAALLPGGARGFLTPDRRLGRWPWRGLLLALP
                     GLGLTAALVVFCLVEKPAPPQASIFSVTSLCRLPAAMRWPEGSAVLSPPVLGAALVAQ
                     LPHPGVIAIPNHHAAPGMDRVDRFLDPGNRDPREALDESRATHVVLCAAPELPGQALR
                     ARFPFAFSLMDGQPPQWLSRCPLEAGSSLRIYSYRRADGSAAACPSR"
     gene            1062..2156
                     /locus_tag="VGM46_02425"
     CDS             1062..2156
                     /locus_tag="VGM46_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEY4191477.1"
                     /translation="MNIKSLLFGSAAALVAVSGARAADAVVVAEPEPAEYVRICDVYG
                     AGYFYIPGTETCLRVGGYVRMDISGGDAGSFDGRDTVDVRDGRARRSYGIQSRFALKT
                     WTGQETELGTLKTYTETRFNYTNGTTSTDAHYAWIQLGGLRLGVDESAYDTFQGYAGN
                     VIQDTLVPYGGFGTNLISYTFDAGNGFSALVSLEQGNNADGNGFDVLDSYTPHVVGGV
                     KYTAGWGGISGVAAYNSNWEEWAGKIRLDVNATEQLSLFIMGGYGSDDNVARNFYKPW
                     GGNWALWGGGTYKINEKTSLNAQVSYDEDETLGVAANVSYDIVPGFNVTAEVNYVNEG
                     DHVAGAGSWTGVPAGDDDAIGGIVRFQRSF"
     gene            2602..3615
                     /locus_tag="VGM46_02430"
     CDS             2602..3615
                     /locus_tag="VGM46_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003527004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HEY4191478.1"
                     /translation="MTKTQPADNPPPSAQAGLRFAVLVFPGFPLMAFSSVIEPLRAAN
                     TLSGKPCYSWMTVGLHDGKVFASNGVGIDPDQSVRAAPLVDRIVVCSGGDADQLVAED
                     AVSWIRRNLRAGAHIGAVADAAFFLARAGLLQGHACTLHWTSQPAFKEAFPELDMKRD
                     LYVIDRRRFTSAGGVGGLDMMLEMISTDYGAELAAGVAEWFVHSPLRSGVDRKMMPIR
                     LRTGIRDQLVLSAVAIMEDAVEDRLSMSDLARRLKISPDRLERAFRLELQLTPGSYYR
                     SLRLRRAADLLTHSSLPIGEVALSCGFSNASSFARAFKEQFGHPPNEVRRRKSISGEA
                     PRA"
     gene            3823..>4089
                     /locus_tag="VGM46_02435"
     CDS             3823..>4089
                     /locus_tag="VGM46_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muconate cycloisomerase"
                     /protein_id="HEY4191479.1"
                     /translation="MVKISAIRVRPLVLPLKQPYHWSYGIRENFAVNLIEIEADDGTV
                     GIGECTVAPDQGGTAAILSRLASHLVGHSPYDGVPLISRIFHQEY"
BASE COUNT          734 a         1277 c         1240 g          838 t
ORIGIN      
        1 ttgccgttct tctccggcat cggcctgctt ttcatcgttg ccgccctgtt gccgggtgga
       61 gcgcgcggtt tcctcacgcc agatcgccgg cttgggcgct ggccctggcg cggcctcctg
      121 ctcgcccttc cgggccttgg cttgaccgcg gcgctggtcg tgttttgcct tgtggaaaaa
      181 ccggcgcccc cgcaagcttc aatcttcagc gtgaccagcc tttgccgcct gccggccgcc
      241 atgcggtggc cggaaggttc ggcggtgctt tcgccgccgg tgctcggggc cgcgcttgtc
      301 gcgcaactcc cccaccccgg cgtcatcgcc attccgaacc accacgccgc tcccggcatg
      361 gaccgggtgg accgtttcct cgatcccggc aaccgggacc cccgcgaggc actggacgaa
      421 agccgggcga cacatgttgt tttatgcgcc gcgcccgaac tgcccggcca ggcgctacgc
      481 gcgcgttttc cgttcgcctt ttcgctgatg gacgggcagc cgccgcaatg gctgtcgcgg
      541 tgccccctcg aagccggctc atcgcttcgc atctacagct acaggcgcgc ggacggttct
      601 gcggccgcct gcccctcgcg ctgaactcga atgattcgtg gccgaacgac aggcgaaccg
      661 ctgtttcctt cgcccagcac gaagcgccga acgacaggca ccgcgtgcga ttcgcctgaa
      721 atctacatcc gaacgatcca tttcgaggga ttcgccgccc ggaacgcatc cgcgaggcca
      781 tgccagcctg tttgccggcc gttggcgcgc cccgtttatt aaagaagagt ttaagcatcg
      841 cccaaaatgt gccctttctg caacagacct atccgatagc accccgacat cgctttcatc
      901 ggtacgaacg tggttgaacg ggacggaccg atgggtaatg tcaggtcaga ggaaggggcg
      961 cggtgcgcat ctttttcggt agtggggatg gggcagggaa cgctgtcctt cagcaaaaaa
     1021 tacccagacc cgttttttaa cttttgactg gaggtcagaa aatgaacatt aagagccttc
     1081 ttttcggctc cgctgcggct ctcgtcgcag tttccggcgc acgcgcagcc gacgctgttg
     1141 ttgtcgccga gccggaaccg gctgaatacg ttcgcatctg cgacgtgtat ggcgcgggtt
     1201 acttctacat ccccggcacc gagacctgcc tgcgcgttgg cggctatgtc cgtatggaca
     1261 tcagcggtgg cgacgccggt tccttcgacg gccgtgacac ggtcgacgtt cgtgacggtc
     1321 gggcccgtcg ttcctatggt atccagagcc gtttcgctct gaagacctgg accggccagg
     1381 aaaccgaact cggcacgttg aagacctaca ccgagacccg tttcaactac accaacggca
     1441 ccacgagcac tgacgcgcac tatgcgtgga tccagctcgg cggcctgcgc ctcggcgttg
     1501 acgaatcggc ttacgacacg ttccagggtt atgccggtaa cgtgatccag gatacgctgg
     1561 ttccttacgg cggcttcggc accaacctga tcagctacac cttcgacgcc ggcaacggct
     1621 tctcggctct cgtgtcgttg gagcagggca acaatgctga cggtaacggc ttcgacgtgc
     1681 tcgacagcta caccccgcac gttgttggcg gcgtgaagta caccgctggc tggggtggca
     1741 tttccggcgt tgctgcttac aattccaact gggaagagtg ggctggcaag atccgtttgg
     1801 acgtgaacgc taccgagcag ctgtcgctgt tcatcatggg cggctacggt tcggacgaca
     1861 acgtcgctcg caacttctac aagccttggg gcggcaactg ggctctgtgg ggcggtggca
     1921 cctacaagat caacgaaaag acttctttga acgcccaggt ttcgtatgac gaagacgaga
     1981 cgctcggtgt tgctgcgaac gtgtcttacg acatcgtccc cggcttcaac gtgacggccg
     2041 aagtcaacta cgtcaacgaa ggcgaccatg tcgctggtgc tggcagctgg accggtgttc
     2101 ctgctggcga cgacgatgcc atcggcggca tcgtgcgctt ccagcgctcg ttctaagaca
     2161 gtcctttctg tctaaaagaa aacccggcgg gcaaccgccg ggttttttta ttgcgtaagt
     2221 ttgccggggc gcggctctac ggcacggacc ggcggcaccg tccacatgaa acacaccgca
     2281 tcgggccggc cgatgatctt cgaggacatc gacaggctcg tgctttcgcg gagacatgac
     2341 cgggtgagtt gcactgaacg ccgaacttga tgactatccg ggaaaaatgg acgctatcgg
     2401 caatgcgtcg acgccgtaca gcgccgagga agccgcgttc gttggctgaa tggacgccga
     2461 acgtcatggc tattcgggaa agatgggcat tatcggcaat gcatcgacgc catgcagcac
     2521 tgaggaagcc gtgttcgaag gcctgaagac cggacttgcg atgcgaacgc gaatacgcca
     2581 attgccgcgg acagcgacca gatgacgaaa acccagcctg ccgacaaccc accaccatcc
     2641 gcgcaggccg gcttgcgctt tgccgtgctg gtttttccgg ggtttccgct gatggcgttc
     2701 agttcggtga tcgaaccgct gcgggcggct aacaccctgt cgggcaagcc ttgttattcg
     2761 tggatgacgg tgggcctgca cgacggaaaa gtgtttgcct cgaacggcgt cggtatcgac
     2821 cccgaccaat cggtgcgcgc ggcgcccctg gtcgaccgta tcgtggtgtg ttccggcggc
     2881 gatgccgacc agcttgtcgc cgaggatgcc gtcagctgga tccgccgcaa cctgcgggcc
     2941 ggcgcgcata tcggcgcggt tgcagacgcc gcgtttttcc tggccagggc cgggcttctg
     3001 caaggtcacg cctgcacgct gcactggacc agccaaccgg ctttcaagga agcctttccc
     3061 gaactcgaca tgaaacgcga cctttatgtc atcgaccggc gacggtttac gtctgcgggt
     3121 ggcgtcggcg gtctcgacat gatgcttgag atgatctcga cggattatgg ggcggagttg
     3181 gcggcgggtg tcgccgaatg gttcgtgcac agcccgctcc gctccggcgt ggaccgcaag
     3241 atgatgccga ttcgactgag gaccggcatt cgagaccagc ttgtcctgag cgcggtggcg
     3301 atcatggaag acgccgtcga agaccgtttg agcatgtccg acctagcgcg gcgcctgaaa
     3361 atatcccccg acaggctgga gcgcgccttc cggcttgaac tccaactcac gccgggcagc
     3421 tactaccgct cgttgcgcct gcgccgcgcc gccgatctcc tgacgcactc gtcgctgccg
     3481 atcggtgagg tggcgctttc ctgcggcttt tccaacgcat cgagcttcgc gcgcgccttc
     3541 aaggagcagt tcggccaccc gccaaacgaa gtgcggcgac gcaaatccat atccggcgag
     3601 gctccgcgcg cctgagatgc cagcaaccgg tgtcggccgg tatactgcct attcacaatg
     3661 ccactgatat ccgcatatca aatgcggctt ttcgcatatc gttggccgat atcatcgtcc
     3721 attctctgcg cggtgaagct gcctcgttcg tgtccgacag ccattcacgg tgttcaatat
     3781 gcgccggcag tttcatgcgg gcgtcaattc acggaagaaa agatggtcaa gatatccgcc
     3841 attcgcgtca ggccgctggt gcttcccctg aagcagccct accactggtc ctatgggata
     3901 cgggagaatt ttgccgtcaa cctgatagag atcgaggccg atgacggtac ggtcggcatc
     3961 ggtgaatgca cggtggcgcc cgatcaaggt gggacggccg ccatcctttc gcggctggct
     4021 tcacatctgg tcggacattc gccttatgac ggcgtgcctt tgatctccag aatattccat
     4081 caggaatat
//
LOCUS       DASYCS010000066         2881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3638577,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000066 DASYCS010000000
VERSION     DASYCS010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3638577"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(208..420)
                     /locus_tag="VGM46_02440"
     CDS             complement(208..420)
                     /locus_tag="VGM46_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006338270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEY4191480.1"
                     /translation="MATGTVKWFNSTKGFGFIQPDNGGADVFVHISAVERAGLSTLVE
                     GQKINFEIEQDRRTGKSSAGSLSKAA"
     gene            444..710
                     /locus_tag="VGM46_02445"
     CDS             444..710
                     /locus_tag="VGM46_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191481.1"
                     /translation="MFVDWAKAQPVCIEILEIDVSKREDREARIDRPKYQCLTYRPGG
                     CQMQDKGFAGQGFLQERGFPGSDETAMARCAFRLRFGIRVRRIT"
     gene            728..1048
                     /locus_tag="VGM46_02450"
     CDS             728..1048
                     /locus_tag="VGM46_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191482.1"
                     /translation="MKKALIIIGLCLVSASAQAITRHDPTKISCDQVHETIAGEGAVI
                     LRYQSTRTPGLPLYDRYVSDQRFCERDEVAYPATVPTADTKSCPVKVCKVLDLDDRQG
                     VLMP"
     gene            complement(1045..1620)
                     /locus_tag="VGM46_02455"
     CDS             complement(1045..1620)
                     /locus_tag="VGM46_02455"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEY4191483.1"
                     /translation="METAPQTAAGNAVFETALGFMGLAWSERGISRVFLADKSSAAVE
                     RRLLRHCGQTPTAERDRPRWIAELIASIKRYAAGDEVDFSAVPVDLAGVDDFRLAIYA
                     AARRLDFGQTTTYGELAKRAGHGGLPRETGAALGSNPVPLIIPCHRILAAGGKIGGFS
                     APGGSAAKERMLELEGVRVGPPPAAQASFGF"
     gene            1807..2646
                     /locus_tag="VGM46_02460"
     CDS             1807..2646
                     /locus_tag="VGM46_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008877586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY4191484.1"
                     /translation="MPALKTLSSMGLIVAAAGLCGCTTASNTAKPQLAETAGLAGQDA
                     GFALPLPETVSILPESAFARAASNADLAALPPPLQPGEQLAAAQAGAQASPVVLASAT
                     PGFEPVGYAAPSSPAEANTGAVDQLIAKYAAVYELPEAFVRRVVKRESTFNPRAYNHG
                     HWGLMQIKHATARGMGYLGPASGLFDAETNLKYAVKYLRGAWLVADRDENRADWLYQT
                     GYYYDAKRKGMLGETGLGVDRRRSRRAPGVAATLPVAGQIGVPQTPVTAPVPAFSSTL
                     PDA"
     gene            complement(2658..>2881)
                     /locus_tag="VGM46_02465"
     CDS             complement(2658..>2881)
                     /locus_tag="VGM46_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191485.1"
                     /translation="RANELKARKALAPSTLRRMINYFARHEVDKKGKNYNNAENPSAG
                     HIAWLLWGGDEGRAWAIELKQRIGNAPDI"
BASE COUNT          528 a          915 c          886 g          552 t
ORIGIN      
        1 gtaacaggag gcaaataaaa aggcgggata aatcccgcct tcccatttcg gtagcctgac
       61 cgggagcttt ctgaccgggc cggcagcaat ctgctgcgtc ggcttgtcgg gttcttcatg
      121 ctccggcgcg cttatcgcgc gaccgccagt acatccgtga cttgccgcgc gctccggact
      181 atcgcccggc gcgcgccgtt ttcaaaatca ggctgccttg cttagagaac cagcggacga
      241 cttgccggtg cggcggtcct gctcgatctc gaagttgatt ttctggcctt cgaccagagt
      301 ggagaggcca gcgcgctcaa cagcggagat gtgaacgaaa acgtccgcgc cgccattgtc
      361 aggctggatg aagccgaaac ccttggtgga attaaaccac tttaccgttc cagttgccat
      421 gtcgatagtc cttcgtattt gctatgtttg ttgactgggc caaggctcag ccggtgtgca
      481 tcgagatttt ggagatagac gtcagcaaac gcgaagatcg cgaggcccgg atcgatcggc
      541 cgaaatatca atgcctcaca tatagaccgg gtggatgtca aatgcaagac aagggctttg
      601 cagggcaggg atttttgcaa gaacgaggct ttccgggcag cgatgaaacc gccatggcgc
      661 gctgtgcgtt tcgccttcgc tttggtattc gtgttcgacg catcacttga aattcctgga
      721 gcaagccgtg aaaaaagccc tgatcattat tggcctttgc ctggtttccg ccagcgcgca
      781 ggcgattacg cgccatgatc cgacgaagat aagctgcgac caggtgcatg aaacgatcgc
      841 gggcgagggt gcggtgatcc tgcgctatca gtcgactcgc acgcccggcc tgccgcttta
      901 tgaccgctat gtcagcgacc agcgtttttg cgagcgcgac gaggttgcct atccggccac
      961 cgtgcctacc gccgacacaa aatcctgccc ggtcaaggtg tgcaaggtgc tggatctcga
     1021 cgaccggcag ggcgttctca tgccctaaaa cccgaacgag gcctgcgctg ccggcggcgg
     1081 gccgacgcgc acgccttcca gctccagcat ccgttccttg gcggccgagc cgcccggagc
     1141 ggaaaagccg cctatcttgc cgccggcggc aaggatgcgg tggcacggaa tgatcagcgg
     1201 caccggattg gagccaagcg ctgcgcccgt ttcgcgtggc aggccgccat ggccggcgcg
     1261 cttggccagt tcgccataag tggtggtttg cccgaaatcg agccttcgcg ccgccgcgta
     1321 gatggcgagg cggaaatcat cgacgccggc cagatcgacc ggcaccgccg aaaaatccac
     1381 ctcgtcgccc gccgcatagc gctttatcga ggcgatcaac tccgcaatcc atcgcggcct
     1441 gtcccgctcc gccgtcggcg tttgcccgca atggcgcagc aggcgccgct cgaccgcagc
     1501 gctgctttta tcggccagga agacgcgcga aatgccgcgt tcgctccacg ccaggcccat
     1561 gaagccgagc gcggtttcaa agacggcgtt gccggcggcg gtttgaggtg cggtttccat
     1621 atgatgctcc cctgtcttga taatcgcttg gccggtgcaa tcggatcata tcgggaacat
     1681 atcgtatcga ttggcggctt ttccacccga aaaccgacga aacccggcaa agattgccga
     1741 ttggtggagg ctggtgtatg cacaggccaa atcagcattc cgaaaaacca cagccaaggc
     1801 ttcgaattgc ccgcattgaa gaccctttcc agcatgggcc tgatcgtggc cgcagccggc
     1861 ctttgcggct gtacgacggc ctcgaacacg gccaagccgc aattggccga gaccgccggc
     1921 cttgccggcc aggatgccgg ctttgcgctg ccgctgccgg aaaccgtctc catattgccc
     1981 gaaagcgcgt ttgcccgcgc ggccagcaat gccgatcttg ccgccttgcc gccgccgctc
     2041 cagcccggcg agcaactggc agccgcccaa gccggcgccc aggcatcgcc cgtcgtgctg
     2101 gcgtcggcca cgcccggttt cgagccggtc ggctatgcag cgccttccag cccggccgag
     2161 gcgaataccg gggccgtcga ccagctgatc gccaaatatg ccgccgtata tgagctgccg
     2221 gaggcgttcg tgcgccgcgt cgtcaagcgc gaaagcacgt tcaacccgcg cgcctataat
     2281 cacggccatt gggggttgat gcagatcaag cacgctaccg ccaggggcat gggctatctc
     2341 ggcccggcga gcgggttgtt cgatgccgag accaatttga aatatgccgt caaatatctg
     2401 cgcggcgcgt ggctggtggc cgatcgcgac gagaaccgcg ccgactggct ataccagacc
     2461 ggctattatt acgacgccaa gcgcaagggc atgcttggcg aaacgggcct tggcgtcgac
     2521 cgccggcgct cgcgccgcgc gccgggcgtt gccgccacgc ttccggtagc aggccagatc
     2581 ggcgtgcccc aaacgccggt aaccgcgcct gtgcctgcgt tttcctccac tttgccggac
     2641 gcctgagcgt accgcgctca aatgtcgggc gcattgccta tgcgctgctt cagttcaatc
     2701 gcccaggcgc ggccctcgtc gccgccccat agcagccatg cgatatggcc ggcggatgga
     2761 ttttcggcgt tgttatagtt cttgcccttc ttgtcgacct cgtggcgagc gaaatagttg
     2821 atcatgcggc ggagggtgga aggggcaagc gccttgcgcg ccttcagttc gttggcccgc
     2881 g
//
LOCUS       DASYCS010000067         2619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1609565,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000067 DASYCS010000000
VERSION     DASYCS010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1609565"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1290
                     /locus_tag="VGM46_02470"
     CDS             <1..1290
                     /locus_tag="VGM46_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006200028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEY4191486.1"
                     /translation="GRALSPDVSAKYLNSPETELFHKGNVLYNFARARKALAKGGTII
                     AVEGYMDVIALAQAGFDNVVAPLGTALTENQLELLWRMSGEPVLCFDGDQAGLKAAWR
                     AADLALPAIQPGRSARFALLPEGKDPDDLVKAEGPDAFRAVLSEARPLAELLWMRETA
                     GGVFDTPERRAELEKTLRELTGRIRDESVRFHYNQDMRDRVHSFFGAQRGRQDRSGNF
                     GGRGKGAGQAGQFGGGQFARGGQAGGRTAISESLGRSAMVKRSGEMMSAREATIIVAL
                     VNHPTLIDENFAHVEFLDLANADLRRLHATLLDAMAHDMANDREAVVATIERAGGSAI
                     WERAVSLIKRTRQWPALESASLDDAREAFSQALHLHRSARTLHKELKQAESALATDPT
                     DENYRHLIEIQAQFRDVQATEALIEGFGVTSGRTGRV"
     gene            1604..>2619
                     /locus_tag="VGM46_02475"
     CDS             1604..>2619
                     /locus_tag="VGM46_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008936102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
                     /protein_id="HEY4191487.1"
                     /translation="MATKEKEEVETEREGTTDGPLLDLSDDAVKKMIKVAKKRGYVTM
                     DELNAVLPSEEVTSEQIEDTMAMLSDMGINVVEDDEQGEEAEGGENAADAEEDANELA
                     ERTGTAVVAAATKKEPTDRTDDPVRMYLREMGSVELLSREGEIAIAKRIEAGRETMIA
                     GLCESPLTFQAIIIWRDELNESKILLREIIDLEATYAGPEAKQAPVVERVEEVKVEDK
                     QRRSREDEEDITNVGADTRGLGDDDDEDEDEASLSLAAMEAELRPQVMETLDVIADTY
                     KKLRKLQDQQVENRLAAAGTLSPSQDRRLKELKDQLIKAVKSLSLNNARIEALVEQLY
                     DINKR"
BASE COUNT          552 a          761 c          888 g          418 t
ORIGIN      
        1 gggagggcgc tgtcgccgga cgtctcggcg aaatacctca attcgccgga aacggaactc
       61 ttccacaagg gcaatgtgct ttataacttc gcccgtgccc gcaaggcgct ggcgaaaggc
      121 ggcacgatca tcgccgtcga aggctatatg gatgtgatcg cgctggcgca ggccggtttc
      181 gacaatgtgg tggcgccgct cggcacagca cttaccgaaa accagctcga acttttgtgg
      241 cgcatgtcgg gcgagcctgt cttgtgcttc gacggtgacc aggccggcct caaggcggcg
      301 tggcgcgccg ccgatctggc gctgccggcg atccagcccg gacgcagcgc gcgctttgcc
      361 ttgttgcccg agggcaagga tcccgacgat ctggtcaagg ccgaggggcc ggatgcgttt
      421 cgcgccgtgc tttcggaggc gcggccgctg gccgagcttt tatggatgcg cgagacggcg
      481 ggcggcgtgt tcgatacgcc ggagcggcgg gccgagctgg aaaagacgct ccgtgaactg
      541 accggccgca tccgcgacga gagcgtgcgc tttcactaca atcaggacat gcgcgaccgg
      601 gtgcacagct tcttcggcgc gcagcgcggg cgccaggatc ggtctggcaa ttttggcggt
      661 cgcggcaagg gcgcggggca ggccggccaa ttcgggggcg ggcagtttgc tcgcggtggg
      721 caggccggcg ggcgcaccgc catcagtgaa agccttggcc gctcggcgat ggtcaagcgc
      781 tccggcgaga tgatgtcggc gcgcgaggcg acgatcatcg tggcgctggt caaccacccg
      841 acgctgatcg acgagaattt cgcgcatgtc gaattcctcg atcttgccaa tgccgattta
      901 cggcggctgc acgccacgct gctcgacgcc atggcgcatg acatggccaa tgaccgcgaa
      961 gcggtggtcg ccaccatcga gcgggccggc ggcagcgcga tttgggagcg cgcggtttcg
     1021 ctgatcaaac ggacgcggca gtggccggcg ctggaatccg ccagtcttga cgatgcgcgt
     1081 gaagcgttca gccaggccct gcacttgcac cgcagcgcgc gcaccttaca taaagagctg
     1141 aaacaggccg aaagtgcgct ggccaccgac ccgaccgacg aaaattaccg gcatctcatc
     1201 gagattcagg cgcagtttcg cgatgtgcag gcgaccgaag cgctgatcga aggctttggc
     1261 gtgacttccg ggcgaacagg ccgggtgtga tgcgtaaaat agctaaggcg gcaattgatt
     1321 cgctttttta acgcgaatca tgcgacaggc gcttgacctt ccgaaggaat gacggaatca
     1381 gaacgattcg aaacgttaac ccgatccagc gtcaacgaaa cacgcagatg cagggtatgg
     1441 gctactggac gggtgagaac cccgccacag atatcagggt taatctggac ttaacgcgaa
     1501 atgcggcaat tgggacggtg agtgcgcttt tcagcggacg cccttagcat acccgtttga
     1561 tgctttccgc cccgccggac ggccgtttgg agaaaaagag agaatggcga cgaaggaaaa
     1621 ggaagaggtc gagaccgagc gcgaaggcac gaccgatggt cccttgctcg acctttccga
     1681 tgatgctgtc aagaaaatga tcaaggtcgc caagaagcgc ggctatgtga ccatggacga
     1741 actgaacgcc gtgctgcctt cggaggaggt gacctccgag cagatcgagg acactatggc
     1801 gatgctgtcc gacatgggca tcaacgtggt cgaggatgac gagcagggcg aagaggccga
     1861 aggcggcgaa aacgccgccg acgccgagga agacgccaac gaactggccg agcgcaccgg
     1921 cacggcggtc gtcgcggccg ccaccaagaa agagccgacc gaccgcaccg acgaccccgt
     1981 gcgcatgtat ctgcgtgaga tgggatcggt ggagcttctg tcgcgcgagg gcgaaattgc
     2041 catcgccaag cgtatcgagg ccggccgcga gacgatgatc gccggcctgt gcgaaagccc
     2101 gctgaccttc caggccatca tcatctggcg cgacgaactc aacgaatcga aaattttgct
     2161 gcgcgagatc atcgaccttg aggcgaccta tgccgggccg gaagccaagc aggcgccggt
     2221 cgtagagcgt gtcgaggaag ttaaggtcga ggataagcag cgccggtcgc gtgaggacga
     2281 agaagacatc accaatgtcg gcgccgatac gcgcggcctg ggcgatgacg acgacgagga
     2341 cgaggacgaa gccagcctgt cgctggcggc gatggaagcg gaactgcgcc cgcaggtgat
     2401 ggagacgctg gacgtcatcg ccgacaccta caagaagctg cgcaagttgc aggaccagca
     2461 ggtcgagaac cgcctggccg ccgccggcac gctttcgccg agccaggatc gtcgcctgaa
     2521 ggagctgaag gaccagctca tcaaggcggt gaagtcgctg tcgctcaaca atgcgcgcat
     2581 cgaggcgctg gtcgagcagc tttacgacat caacaagcg
//
LOCUS       DASYCS010000068        10522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3494610,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000068 DASYCS010000000
VERSION     DASYCS010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 10522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10522
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3494610"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="VGM46_02480"
     CDS             complement(<1..993)
                     /locus_tag="VGM46_02480"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019996803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY4191488.1"
                     /translation="MQHGSLSNIKVIDASRVLGGPYCGQILADHGADVIKIEPPTGDE
                     TRGWGPPFQEGTASYFLGLNRNKRGMALDMSQPAGQELLRTLLETADVFIENFKTGTL
                     ERWGLTREEITSRFPRLVHCRVSGFGSDGPMGGMPGYDAAIQASCGIMSVNGEAGGQP
                     LRVGLPVVDMVTGLNAALGILMALNERSVSGKGQFVEAALYDCGVSLLHPHLPNYYLS
                     GKVAAPSGNAHPNICPYDTFATADIPIFLAVGNNRQFVTLCEIIGRSDLPGDARFTNN
                     GERNANRDALKVELEKALAAFNCHELSARLMKAGVPNGAVRTIDQVVADPHTKHR"
     gene            complement(1017..1793)
                     /locus_tag="VGM46_02485"
     CDS             complement(1017..1793)
                     /locus_tag="VGM46_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY4191489.1"
                     /translation="MENVVPREILIASIARLLDGVRHVAVGASSPIPAAGAMLLRAQK
                     EQAGQPPVRISILGSVEHNFFTNGSTELFDCAGQGRIDAFFLGGGQIDGQGNINLVGI
                     GEYPQSKVRWPGSFGSAYLYYVVPRVILFREEHTPRVFVDKVDFISAPGLSEEGVYRT
                     GGPYALLTGMGLFRFDKEKRGFALESVHPGRSLSDIRGATGFEFDHAAEPRVTELPDS
                     ETLSLLRGRVLDELSETYPEFASQMREEIHALQARPKSIS"
     gene            complement(1794..2579)
                     /locus_tag="VGM46_02490"
     CDS             complement(1794..2579)
                     /locus_tag="VGM46_02490"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEY4191490.1"
                     /translation="MAGLVQKGMKIALPVDYAGVSMAMTSALIESGVGELHIICVPTG
                     GLQVDQLVGAGLVATVETSAVSLGEAGGAPRFNQAVKDGSIRVLDATCPAIHAGLMAA
                     QKGAPFMPMRGLIGSDILRFRPDWPVISNPLSEQDDPVVLIPALTPDIAIFHAPMADR
                     FGNVWIGRRRELAAMAYASRQTLVTVERITDENLLASEMTAAGTLPAIYVDAIALSPG
                     GARPYGLWGEYAPDAAEIRRYASAAKTQEGFAEYMSGQRAEIA"
     gene            3034..3789
                     /locus_tag="VGM46_02495"
     CDS             3034..3789
                     /locus_tag="VGM46_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019996797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEY4191491.1"
                     /translation="MDKAFIKGLRLVEMLALSEKPRGVTDLAGELRFTKSNVHRLLST
                     LQSEGYVRQVPIGNAYELTTKILELGHNVNRNMSLLQIARPAMTELAKKTGETVHLSV
                     LDGTDVVYIDKIESAHHIRAHTSVGMRAPAFTMATGKAMLAHLPDSYLERFTPHFKAY
                     TGTTRVTLESLREDVEIARRKGHAVVAHGEWREGIAACAAAILGRSGELVGAIGMSGP
                     DTRVKRKQLKDYGEDVMEAARTISSALGYVARG"
     gene            3826..5268
                     /locus_tag="VGM46_02500"
     CDS             3826..5268
                     /locus_tag="VGM46_02500"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008597676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY4191492.1"
                     /translation="MARNYPDTKLFIDGEWRNARDNRTIPVIDPATEEVIGHVAHAGR
                     ADLDDALAAAEKGFAVWSKTSAHERYKLMRNAADLLRKRVAEIAPIMTMEQGKPLAES
                     QGEISAGADIIDWFAEEARRTYGQVIPARVPGVTQMTIKLPVGPVAAFTPWNFPINQA
                     VRKLSAAVAAGCSIIIKAPEETPASPAALVQAFVDAGIPNGVVNLVYGVPSEISEYLI
                     PHPVIRKISFTGSTEVGKHLAALAGRHMKRVTMELGGHAPAIVCDDADIERAVKVFGG
                     YKYRNAGQVCISPTRFLVQDKVFDEFLDGMVTMAKAVKIGNGMDPDTQMGPLANERRI
                     PALEGLIADAVSHGAEVKTGGRRVGNKGLFFEPTVIANAPISARIMNEEPFGPIAVIN
                     RFSTLDDAIEEANRLPFGLASYAFTRSAETSEQLGLRIDAGMTTINHLGLALPEVPFG
                     GVRDSGYGTEGGSEAIDAYIVTKYVTRMAG"
     gene            complement(5401..6294)
                     /locus_tag="VGM46_02505"
     CDS             complement(5401..6294)
                     /locus_tag="VGM46_02505"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY4191493.1"
                     /translation="MNIRQLRTLSEFVDQGSFASTGDRVGLSPSAISIQMSRLEEDLG
                     VALFDRGTRPPTLTAEGLAITKLAREMIELEAKIKRIARGDLVKVVTIGFVPTTLTKI
                     LPEIVGQLRDTYADLQINIKSGLSGELAAAVLRYEMDFALITAPLGDSLELSVCEIVR
                     EPLFAVGPLSLGHIETDAELITALPFIFFNKRAWLGQKIAAELQARAIFPHEGMEVDS
                     LDTIEHLVSQGFGVSVVPQRLLASPLSERLNCVPFGSPQQARSLALIEHAKRRKTDLD
                     DAVVSIFKSLTDFAPQNGRAS"
     gene            6786..7574
                     /locus_tag="VGM46_02510"
     CDS             6786..7574
                     /locus_tag="VGM46_02510"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HEY4191494.1"
                     /translation="MACLSSKNALVTGSTSGIGLAIARAFAAEGANVTINGLGDAAAI
                     EQERAGIEREFAVKCRYSDANMMDGPAVSAMVREAEQAFGGLDILVNNAGIQHVAPIE
                     DFPDDKWEAIIRINLLAAFYAIKAALPGMKARKWGRIINTASAHALVASPFKSAYVSA
                     KHGIAGLTKTVALETAQDGITVNAIAPGYVWTPLVERQIPDTMKARGLTEEQVKRDVL
                     LAAQPTKEFVSVEALGALALFLCSDAARQITGATLPMDGGWTAQ"
     gene            complement(7862..8236)
                     /locus_tag="VGM46_02515"
     CDS             complement(7862..8236)
                     /locus_tag="VGM46_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191495.1"
                     /translation="MTAFRYLFVALVPLVLAGCISDEDQRQMDQEKCDSYGFRPGSNS
                     FASCMMKQNERRADDEQRYLDRSRADEQRKRDRRRSEKQSSYIDPTPQYDRDGNPNFD
                     TKGGYIGCNGIGCTIDNPDDDN"
     gene            complement(8426..8788)
                     /locus_tag="VGM46_02520"
     CDS             complement(8426..8788)
                     /locus_tag="VGM46_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HEY4191496.1"
                     /translation="MRLFITMTTIPLLMASAALAQSPGADFQKADKALNRNFKQIVTR
                     LSDDPSGKARLVRSQRAWVAFRDAECAFQSSGDDGGSAAPMIVATCRTDLTTQRTKQL
                     RAYLNCEEGDLSCPVPSN"
     gene            9092..>10522
                     /locus_tag="VGM46_02525"
     CDS             9092..>10522
                     /locus_tag="VGM46_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018429889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-hydrolase family protein"
                     /protein_id="HEY4191497.1"
                     /translation="MFRQWIVRYGYSFSMTGLLLGTLFFAASLTPTLIPRNYLTQGVL
                     SGLSTAAGYGLGFFLRWLWRYLELPEFPARTQKTSKICAALACLAVALVFLWRAAGWQ
                     NSIRHLMYLDPVDSAHPFEVGAIAILAFMALVALARLFYLVLDFISGQLARFVPRRIS
                     YVVGTAAAILLFWSAANGLIFRYGLRALDSSFQRVDAMMAPETRQPDDPARTGSAASL
                     VRWQDLGRMGRSYIASGPTAADIGKFTGKPATEPVRVYVGLASGETAMDRARLALEEL
                     KRAGGFERSALVIITPTGTGWVDEQAIDPIEFLHNGDVASVGAQYSYLASWMSLLVEP
                     GYGAEQSRALFSEVYGYWHTLPRDKRPKLYLFGLSLGSLSTELSNELFEVLGDPYQGA
                     LLSGPPFASRIWRSLTDRREKGTPEWLPRFRDGSYVRFTAQKDALDIPGARWGPLRVV
                     YLQYASDPITFFEPRSFYRRPDWMTGP"
BASE COUNT         2004 a         3211 c         3259 g         2048 t
ORIGIN      
        1 gcggtgcttg gtatgcggat cggcaacgac ctggtcgatg gtcctgaccg cgccgttggg
       61 aacgcccgcc ttcatcagcc gcgccgatag ttcgtggcaa ttgaaggcgg ccagggcctt
      121 ttccaactct actttcagcg cgtcgcggtt ggcgtttcgc tcgccattat tggtgaaacg
      181 cgcgtcgccc ggaagatcgg agcgtccgat gatttcgcac agcgtgacaa actggcggtt
      241 gtttcccacc gcgagaaaga tcggaatgtc ggccgtcgca aacgtgtcgt aggggcagat
      301 gttcggatgg gcgtttccgc ttggcgccgc gactttgccg gacaggtaat agtttggaag
      361 gtggggatgc agcagcgaca caccgcaatc gtaaagcgcg gcctcgacga actggccctt
      421 gccgctgacg ctgcgctcgt tcaaggccat cagtatgcca agcgcggcgt tcaggccggt
      481 gaccatgtcc accaccggca agcctacgcg cagcggttgg ccgccggcct cgccattgac
      541 gctcatgatg ccgcaagaag cctggatcgc ggcgtcatag cccggcatgc cgcccatggg
      601 gccgtccgaa ccgaaaccgg aaacccggca atggaccagg cgagggaagc ggctggtgat
      661 ctcctcgcgc gtgaggcccc agcgctccag cgtgccggtc ttgaagttct cgatgaagac
      721 atcggcggtt tccagcaagg tacgaagcag ttcctggccg gcaggctggg acatgtccag
      781 cgccatgccg cgcttgttgc ggttcagccc caggaagtag cttgccgttc cctcctggaa
      841 gggcggaccc cagccccgcg tctcgtcgcc ggtcggcggt tcgatcttga tcacatcggc
      901 gccgtggtcc gccagtatct ggccgcaata cggaccccca aggacgcggc tcgcgtcgat
      961 gaccttgatg ttggagagtg agccgtgctg catcattgtg ctgtgtcccg ccttggtcat
     1021 gaaatggatt tcggccgggc ttgaagcgcg tggatttcct cgcgcatctg actggcaaat
     1081 tcgggatatg tttcgctcaa ttcatcgagg acgcgcccac gaagcaatga aagagtttcg
     1141 ctatccggca gttcggttac ccgcggttcg gcggcatggt cgaattcgaa cccggtcgcg
     1201 ccccgaatgt cggacaggga acgtccagga tgaacgcttt cgagcgcgaa gccgcgcttt
     1261 tccttgtcga accggaacag gcccatgccc gtgagaagcg cgtaagggcc gccagtgcga
     1321 tagacgcctt cctcgctcaa gcccggagcg ctgatgaagt cgaccttgtc gacgaagacg
     1381 cgcggcgtgt gttcctcgcg aaacaggatc acgcgcggaa cgacatagta cagataggcc
     1441 gatccgaacg aaccgggcca gcgcaccttc gactgcggat attctcctat gccgaccagg
     1501 ttgatgttgc cctggccgtc tatctgcccg ccgcccagga agaaggcgtc gatgcgcccc
     1561 tgtccggcgc agtcgaaaag ttcggtcgag ccgttggtga agaaattatg ctcgaccgaa
     1621 cccaatatcg aaatgcgcac gggcggttgg ccggcctgct ctttttgggc gcgcaacagc
     1681 atcgcgccgg ccgccggaat gggcgacgag gcgcccaccg caacgtgacg gacgccatcc
     1741 agaagccgcg cgatgcttgc gatgaggatt tcgcggggaa cgacgttctc cattcacgca
     1801 atctccgcgc gttggcccga catatattcg gcaaagcctt cctgcgtctt ggccgccgaa
     1861 gcgtagcgcc ggatttcggc cgcgtctgga gcgtattcgc cccacaggcc gtagggtcgc
     1921 gcgccgccgg gcgacagcgc gatcgcgtcg acgtaaattg ccggcagggt cccggcggcg
     1981 gtcatttcgg atgcgagcag gttctcgtcc gtgatgcgct cgacggtcac cagcgtctgc
     2041 cgcgaggcgt aggccatggc ggccagttcg cggcgcctgc cgatccagac atttccgaag
     2101 cgatccgcca tgggcgcatg gaagatggcg atgtcgggcg tcaatgccgg gatgagaacg
     2161 accgggtcat cctgttcgga aaggggattg ctgatgaccg gccagtccgg gcgaaaccgc
     2221 aggatgtcgg aaccgatcaa cccgcgcatc ggcatgaagg gcgcgccctt ctgggccgcc
     2281 atcaggccgg cgtggatggc ggggcacgtt gcatccagca cgcggatgga gccatccttc
     2341 accgcctggt tgaaccttgg cgcgccgccg gcctcgccga gggatacggc gctggtctcc
     2401 accgtggcga caagaccggc cccgaccagc tgatcgacct gaagcccgcc ggttggaacg
     2461 cagatgatgt gcaactctcc cacgccgctc tcgataaggg cgctggtcat ggccatggaa
     2521 acgccggcgt agtcgacagg cagggcaatc ttcatgccct tctgaacaag accagccatc
     2581 gacctgatgt cggacagcac tcattcctcc cgaaacagtg gcagaagccg cgccatgttc
     2641 tacaatgtgg tacgcaattc taaaaaacag cctatcggta gcgggctggc ttgacaagcc
     2701 cataattttc ccggaagact tgtaatcaga aacttgttct atattgtgga acacgaggat
     2761 atccgatggc cggtagcgcg gcaaggtgct gcggacatgc gcgcgggagc gggatgagtg
     2821 aggagcgcgg ccgcaggaag ctgaacgcgc ttccgttgca acgacggtaa attgcaggat
     2881 caacgatgcc ggccccgcct tttttacggc tggtcatgct tttttcgact tgaacttcga
     2941 tggctgcggg gaggggaggc gtgtttgtgt cgtttctgat cttcgtgata ggttggcctg
     3001 ccagaaaccc gccacacctt cggaattcaa cacatggata aagcatttat aaagggtctg
     3061 aggcttgtag aaatgcttgc gctaagtgaa aaaccgcgcg gcgtgacaga tctcgccggg
     3121 gaacttcgtt tcacgaaaag caatgttcac agactgcttt caactcttca gagcgagggc
     3181 tatgtcaggc aggtgccgat tggcaatgcc tatgagctta ccacgaagat cctggaactc
     3241 ggccacaacg tcaatcgcaa catgagcctg cttcagatcg cgcgtccggc gatgacggaa
     3301 ctggcgaaga aaaccggcga aaccgttcac ctgtccgtgc tcgacggcac ggatgtggtc
     3361 tatatcgaca agatcgaaag cgcccatcac atccgcgctc acaccagcgt cggcatgaga
     3421 gcgccggcgt ttacgatggc gaccggaaag gccatgctcg ctcatttgcc cgacagctat
     3481 cttgagcggt ttacgcccca cttcaaggcc tataccggca ccacgcgcgt cacgctcgaa
     3541 agcctgcgtg aggacgtgga aatcgcccgg cgaaagggcc atgcggtcgt cgcccatggc
     3601 gagtggcggg agggcatcgc cgcatgcgcg gccgccattc tggggcgttc gggagaattg
     3661 gtcggcgcaa tcggaatgtc ggggccggac acgcgcgtga agcgcaagca gctcaaggac
     3721 tatggcgagg acgtcatgga agccgcgcgg accatcagtt cggctttggg ttatgtagcc
     3781 cgaggctagg aaataacttt aaatgagacc caggaagcgt tgaaaatggc aagaaactac
     3841 cccgatacga aactgttcat tgacggcgaa tggcgcaacg cgcgcgacaa tcgcaccatt
     3901 ccggttatcg atcctgcgac cgaagaggtg atcgggcatg tggcgcatgc ggggcgcgcc
     3961 gacctggacg acgcgctggc ggccgccgag aagggttttg cggtctggag caagacgagc
     4021 gcccatgagc gctacaagct gatgcgcaac gccgccgacc tgttgcgcaa gcgcgtggcg
     4081 gaaatcgcgc cgatcatgac catggagcag ggaaagccgc tggctgaatc gcagggcgag
     4141 atttcggccg gcgccgatat tatcgactgg ttcgccgagg aagcccgccg cacctatggc
     4201 caggtcattc ccgcgcgcgt gcccggcgtc acccagatga cgataaagct tcccgtcggg
     4261 ccggtggcgg cgttcacccc atggaacttc ccgatcaacc aggcggtcag gaagctgtct
     4321 gccgcggttg cggccggctg ctcgatcatc atcaaggcgc ccgaggaaac gccggcctcg
     4381 ccggcggccc tcgtacaggc attcgtggat gcaggcattc caaacggcgt cgtcaacctc
     4441 gtctacggcg ttccgtccga gatttcggaa tatctcattc cccatccggt catcaggaaa
     4501 atctcgttca ccgggtcgac ggaggtcggc aagcatctgg ccgctctcgc cggcaggcac
     4561 atgaagcggg tgaccatgga acttggcggc catgcgccgg ccatcgtttg cgatgacgcc
     4621 gacatcgagc gcgccgtaaa ggtgtttggc ggctacaaat accgcaatgc cggccaggtc
     4681 tgcatctccc ccacgcgctt cctcgtacag gacaaggttt tcgacgaatt cctcgacggc
     4741 atggtgacca tggcgaaggc ggtcaagatc ggcaacggca tggatcccga tacgcagatg
     4801 gggccgcttg ccaacgaacg ccgcattcca gccctggaag gcctgatcgc ggacgcggtt
     4861 tcgcatgggg ccgaggtgaa gactggtggc cgccgcgtgg gcaacaaggg cctgttcttc
     4921 gagccgacgg tgattgcaaa tgcgccgata tcggcgcgga tcatgaacga ggaacccttc
     4981 ggcccgatcg ccgtcatcaa ccgtttctcg acgttggatg acgccatcga ggaggcaaac
     5041 cgcctgccgt tcggcctggc ctcctacgcg ttcacgcggt cggcggaaac ctccgaacag
     5101 cttggcctgc gcattgatgc cggcatgacc acgatcaacc atctcggact ggcgctacct
     5161 gaagtgccgt tcggcggggt tcgcgattcc ggatatggca cggaaggcgg ctcggaggcg
     5221 atcgatgcct acatcgtcac gaaatatgtg acccggatgg ctggataacc gccaggtcac
     5281 ggccttcatc gcagccaaag cgagcgtgta gcctggatca ggctgcacgt tccgctcgcg
     5341 acttgaccag atttcagcgt ggaagcgagg gcagggtgcc gccgcgccaa gttcgccgcg
     5401 ctacgatgcc cggccgttct gtggcgcaaa gtcggtcagc gatttgaaga tcgagacgac
     5461 ggcgtcgtcg aggtcggttt tgcgccgctt ggcgtgttcg atcaatgcga ggctgcgcgc
     5521 ttgctgcggc gagccgaacg gcacgcagtt cagccgttcc gaaagcggcg atgcaagaag
     5581 acgctgcggc accaccgaca ccccaaagcc ctgcgagacg agatgctcga tcgtgtcgag
     5641 ggaatcgacc tccatgcctt catgcggaaa gatcgccctt gcttgcagtt cagccgcaat
     5701 cttctgcccc agccatgccc gcttgttgaa aaaaatgaac ggcaaggccg tgatcagttc
     5761 ggcatcggtt tcgatgtggc cgagcgacag cgggccgacg gcgaacaggg gttcacgaac
     5821 gatttcgcaa acggacagct ccaaagaatc ccccaacggc gcggtgatga gggcgaaatc
     5881 catttcatat cgaagaaccg ccgcggccaa ctccccagac aagccggatt tgatgttgat
     5941 ctgaaggtcg gcataagtat cccgcaattg cccgacgatt tcgggcaata tttttgtcag
     6001 cgtcgtcgga acgaagccga tcgtcacgac cttgactagg tcgccgcgcg caatccgctt
     6061 tatcttggct tccagttcga tcatttcgcg ggccagcttg gtgatcgcca ggccttcggc
     6121 ggtcaatgtc ggcggtcgcg ttccccggtc gaacagcgca acgccaaggt cttcctcaag
     6181 ccggctcatt tgaatgctga tggcggatgg gcttagcccg acgcggtcgc ccgtcgaggc
     6241 gaaactgccc tgatcgacga attccgacaa agtgcgcagc tggcgaatgt tcacaggagg
     6301 gacctttacg gcaagcacgg ccatttcctg tcatgctatg ccgtatatct ttgaattgtc
     6361 atcaatgatc tttatcattt gaggagattg ttcccctata tccgcgccgc cggacggccg
     6421 aaaatgcttg cttcggggac cgcgcgagcg gcgacaaacg gcgcagttca aacccacggc
     6481 gatgggtcac tcccgcaagc tgccgctcga cagaaacgtg ggtgaagcct tcatgcttcc
     6541 agcggcgcgg tggcgtcccg catcctctca ccgtcatcgg ccgggccgat cttgcaggtc
     6601 ggctcgtcgt tccgcgccac ggcggcgccg tgccggtttc atacggaccg ggcgaccgaa
     6661 gtggcggcgt tgcccttgca atcgcctctg tagtcgcatc gacatatggc cgtggcgctg
     6721 tcatgtttgc gggcgcccga agcgcttcgc ctttcatgtt cctttccacc atcagcgaga
     6781 tcgtcatggc ttgcctgtct tcgaagaatg ccctcgtcac cggctcgacc agcggcatcg
     6841 gccttgctat cgcgcgcgcc tttgccgccg aaggcgccaa cgtcaccatc aacgggcttg
     6901 gcgacgccgc cgcgatagaa caggaacgcg ccggcatcga gcgggaattc gccgtcaagt
     6961 gccgctattc ggacgccaac atgatggacg ggccggcggt cagcgcgatg gtgcgcgaag
     7021 ccgagcaagc cttcggcggc ctcgacattc tcgtcaacaa tgccggcatc cagcatgtcg
     7081 cgccgatcga ggactttccc gacgacaagt gggaagccat catccgcatt aatctgcttg
     7141 ccgcgttcta tgccatcaag gcggcgctgc ccggcatgaa ggcgcgcaaa tggggccgca
     7201 tcatcaacac cgcatcggcc catgcgctgg tcgcctcgcc gttcaagtcc gcctatgttt
     7261 cggccaagca cggcattgcc ggcctgacca agacggtggc cctggaaacc gcacaggacg
     7321 gcatcaccgt caatgccatc gcgccgggct atgtctggac gccgctggtc gaaaggcaaa
     7381 tccccgacac catgaaggcg cgcggcctga ccgaggaaca ggtcaagcgc gacgtgcttc
     7441 tggccgcgca gccgaccaag gaattcgtca gcgtcgaggc gctgggcgcg cttgcgctgt
     7501 tcctctgctc cgatgcggca aggcagatca ccggcgcgac cttgccgatg gatggcggct
     7561 ggacggcgca gtaacgcgca gcgtcgtctc acgccgtttt caatgtccgg cattgccggg
     7621 aaattctgca atttcaggtt gccgacgaaa gcgaaatgcc gggagaaatt ctcccggcat
     7681 ttcgccatat ccgccgcatg gcgcaaacta taggctggcc tgttttccga cgccgatcag
     7741 catagatcgg cccgccggga acggttcaac ggccatctac aatccaacac aagtatcaac
     7801 ggctgctaat attataaatt gccgaaaaag ctgcaaattg cccggcgttt ccggcagcac
     7861 atcaattgtc gtcgtcggga ttgtcgatcg tgcagccaat tccgttgcag ccgatatagc
     7921 cgcccttggt gtcgaagttc gggttgccgt ccctgtcata ttgcggcgtc gggtcaatgt
     7981 agctgctctg cttctcgctt ctgcgccggt cgcgcttgcg ctgctcgtcg gcgcgggaac
     8041 gatccagata gcgctgctca tcgtcggcgc gccgctcgtt ttgcttcatc atgcaggagg
     8101 caaaagaatt cgaaccgggc ctgaagccgt aggaatcgca cttctcctgg tccatctgcc
     8161 gctggtcttc atcgctgatg cagccggcaa gcaccagcgg aacgagcgcg acgaacaaat
     8221 atcgaaatgc agtcatttgg gtccctcact atggtaaaga ctacgaaatc caatctccag
     8281 cgaacgcacc ggcatttcgc aaggcgcggt cacagtcggg tcaggcgtca aagccgagcg
     8341 gagaagcggt tgccgccgca tggccggcct cattgccgtg tccggtcggg aacatttgcg
     8401 gatggggcac gtctcgccgg tatgtctagt tggacggaac cgggcagctc aaatcgcctt
     8461 cctcgcaatt gagataggcc ctgagctgct tggttcgctg ggtggtcaga tcggtcctgc
     8521 atgtggcgac gatcatgggg gcggcgctgc cgccgtcgtc gccgctggac tggaaggcgc
     8581 actcggcatc gcgaaaggcg acccatgcgc gctgcgagcg aacgaggcgg gcctttccgc
     8641 tcggatcatc cgagagccgc gtcacgattt gcttgaagtt tctgttcagc gccttgtcgg
     8701 ctttctgaaa gtcagcgccg ggactttgag caagggcggc agatgccatc aatagaggga
     8761 tcgtcgtcat tgtaatgaat aaacgcatca agtcctccgg ggtgtaatat actggagtta
     8821 ttcacaacag gtatttttat acaacataaa cgtgcgcaaa tggttaagcg aaactcaaac
     8881 gggtagtatt agcgttaagc gtgtttggcg ttggcacgtt tttgcgcgcg ccgtctcctg
     8941 ctatctctat gacgttacag cgcgatgaac ggcccacgcc tgcacgctct ggccacgtcg
     9001 cgcgatttgc ctgagcggcg attttcctga atattgccgg tcggcgaagc gccacattgt
     9061 tcacgcaata agacgacttg gggggagctt gatgtttcgg cagtggatcg tccgctacgg
     9121 ctactccttc tcgatgaccg gcctgctgct cggcacactg ttttttgccg cctcgctgac
     9181 gccaacgctg atcccgcgca attacctgac gcaaggcgtg ctgtccggtc tctctaccgc
     9241 cgccggctat gggctgggct ttttcctgcg ctggctttgg cgttatctgg agctgccgga
     9301 atttccggcg cgcacgcaga aaacctcgaa gatttgcgcg gcgctggcct gccttgccgt
     9361 ggctctggtc ttcctctggc gcgcggccgg gtggcagaat tccatccgcc atctgatgta
     9421 tctcgatccg gtcgacagcg cccacccgtt cgaggtcggc gcgatagcga tcctcgcctt
     9481 catggcgctg gtcgcgctcg ccaggctttt ttacctcgtg ctggatttca tttccggcca
     9541 gctcgcccgt ttcgtgccga ggcgcatatc ctacgtggtg ggaacggccg ccgcgatcct
     9601 gctgttctgg tcggcggcca acggcctcat cttccgctat ggcctgcgcg cgctcgacag
     9661 ttccttccag cgggtagacg ccatgatggc cccggaaacc cggcagccgg acgatccggc
     9721 cagaaccggc agcgccgctt cgctggtgcg ctggcaggat ctcggccgca tgggccgttc
     9781 ctatatcgcc tccggcccga cggcggccga tatcggcaag ttcaccggca agcctgccac
     9841 cgagcctgtg cgcgtctatg tcgggcttgc atccggcgag acggccatgg accgcgcccg
     9901 tctggcgctt gaagaactca agcgcgccgg tggcttcgaa cgatccgccc tcgtcatcat
     9961 cacgccgacc ggcacgggct gggtggacga gcaggcgatc gacccgatcg agttcctgca
    10021 caatggcgat gtcgcaagcg tcggcgcgca atattcctat ctggcaagct ggatgtcgtt
    10081 gttggtcgag ccgggatatg gcgccgagca atcgcgcgcc ctgttcagcg aagtctatgg
    10141 ctattggcat accctgccga gggacaagcg gccgaaactc tatctgttcg gcctgagcct
    10201 cggctcgctt tcgacggaat tgtcgaacga actgttcgag gtgctgggcg acccctacca
    10261 gggcgcgcta ttgagcggcc cgcccttcgc cagccggata tggcgttcgt tgaccgaccg
    10321 ccgggagaaa ggcacgcccg aatggctgcc gcgtttccgc gacggctcct atgtccgctt
    10381 cacggcgcag aaggatgcgc tcgatattcc cggcgctcga tgggggccgc tgcgggtcgt
    10441 atacctgcaa tacgccagcg acccgatcac ttttttcgag cctcgctcgt tctatcgcag
    10501 gccggactgg atgacggggc cg
//
LOCUS       DASYCS010000069         7409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1610074,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000069 DASYCS010000000
VERSION     DASYCS010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 7409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7409
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1610074"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1454)
                     /gene="addB"
                     /locus_tag="VGM46_02530"
     CDS             complement(<1..1454)
                     /gene="addB"
                     /locus_tag="VGM46_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="double-strand break repair protein AddB"
                     /protein_id="HEY4191498.1"
                     /translation="MSDVRRVLTIAPGAPFLPTLAKALLSGTLIPGFRFDGDPLALAN
                     VTIYVPTRRAARALRGELVELMGGGSAILPVIRPLGEFDEDEALFEAQAPESAAALDL
                     APPIPALERLLLLAPLVRAWKRRLPAHVAALFEEEMVVPASTADAIWLARDLAQLMDE
                     IETEGSDWTRLADLVTGNLAGWWQVTLDFLKIVTENWPTLLAELDRSNPAAHRSALIR
                     LEAARLKRNPPAGPVIAAGSTGSIPATAELLSVIAGLPLGAVVLPGLDKVLDDASFAA
                     ISAKDARPALLGHPQYGLAKLIAKIGIVRRDVEQIGDDPLALRTALTGEALRPAETTE
                     RWAETRSQFASSDVDAAFAGVTLLEAANERDEAAAIAIALRHAVEQKGRRAALVTGDR
                     ALARRVSAELLRFGIVADDSGGTPLSQTPPAGLLRLTLGAAFGPGDPVGLLALLKHPL
                     LTVGLERPGVRHAAEVIELVALRGGTGRPDIATLP"
     gene            complement(1451..2155)
                     /locus_tag="VGM46_02535"
     CDS             complement(1451..2155)
                     /locus_tag="VGM46_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015314363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEY4191499.1"
                     /translation="MVLAAGLGRRMRPITETMPKPLVRVAGKTLLDWGLDSLAAAGVE
                     KAVVNVHYFPEQIVAHTFCRSAPKIVISDESDGLLDSAGGIVKALPRLGQEPFYVVNA
                     DTFWIDSGAPSLDRLALAWDDAKTDILLMLADIASATGHSGGTDFLMSPGGALRRAQG
                     GATGLIYAGAAIMHPRIFKNAPVGPLSLNRYFDAAIKAGRLFGMAMHGSWITVGTPEA
                     IPLAEAAVANALSGTP"
     gene            complement(2179..3690)
                     /gene="tsaE"
                     /locus_tag="VGM46_02540"
     CDS             complement(2179..3690)
                     /gene="tsaE"
                     /locus_tag="VGM46_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEY4191500.1"
                     /translation="MAKSVLKRFLADEAATAQLGEDLAMALRPGDVLALSGDLGAGKS
                     TLARALIRALADDAALEVPSPTFTLVQSYDVRPPVHHFDLYRLSSAQELDELGFDEAL
                     ADGAALVEWPDRAEARLPATTVLVELEQDGEGRIARLSGEGAAFERMARSLAMRGFLE
                     DAGWGTAQRRRFVADASARSYEIATLSGFSPRVLMNSPELMLGPPVRDGKPYALIAHT
                     SRTVSAFVAIDRALASAGVSVPEIHAQDLAQGFLLLSHLGSESFLDADGQPVNARYAA
                     AAELLAMMHSRPWHYRLEAAVGVVHAVPPFDRDAMMIEAELLVDWYVPAVSGRPASEE
                     LRQGYQREWDALFARLSGSEYTLMLRDFHSPNIIWRGERQGHDRLGIVDFQDALVGPA
                     AYDVASLAMDARVTIPPETEKAALDAYVAARRGAGGFDEEAFREAYAIMAAQRNSKIL
                     GIFVRLNRRDGKPEYLKHLPRIRDYLRRALGHPALASLREFYVEHGLLEERTL"
     gene            complement(3699..6269)
                     /locus_tag="VGM46_02545"
     CDS             complement(3699..6269)
                     /locus_tag="VGM46_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013891643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY4191501.1"
                     /translation="MPGKTPLHAGKAVSGGRNDSMAGKSGSRCGAGWHRARTMALRAG
                     AALALALPAGIAFGGIAYAEGVSKGSALPISTVDVMQLAVFVGVMAGALISAVVLIRE
                     RGRIAGENVNLKTRLSDANAGLQRADAMLNLRDQRVIVWGPENKKPELIGALPIDSGV
                     PEERAALLAFGRWLMPRSAAMLEHAIAALREKGAPFDFTAETQSGMPLDVNGRKSSAH
                     IIVRFTSLSETQRSQARLKLENQQLVENHETMIGLLSALRMPVWMRAADGRLKWVNRA
                     YAAAVEAEDVDAAVRDAREFLGGQAREAIVEQHRARPVFEQTLSTVIEGDRRKYAVTD
                     FAGPFGSAGLASDTSAMETIRAEYERTMRSHADTLDQLNTAVATFDTDEKLRFFNQAF
                     QKLWGLDSGFLHSAPDNALFLDRLRSEGKIAEQPEWRRWKEGLLCAYRAVEPQEHWWH
                     LPDGKTIRVVANPQPKGGVTWVFENLTEKIDLESRYQTAVRVQGETLDNLAEGVAVFG
                     PDGRLRLSNPAFATLWGLDSGAVKPSVHVSAIRALGDSRAVDSPWGEFAATITGFDEE
                     RRDRHGQTELTNGSILRYAVIHLPNGQVMMTFVDVTDSVNVERALKDKNEALLKSDQL
                     KNDFVQHVSYELRSPLTNIIGFTELLSQPATGPLTPRQRDYVEHVGSSSSVLLTIVND
                     ILDLATVDAGIMQLEISEVPVDRTIAAAAELVADRLDEHSIRLEIDASSAPKSFHGDE
                     TRVRQILYNLLSNAANYAPEAGVIKLACRQSADGVEFSVHDDGPGMPQDVLDAVFRRF
                     EPRVNGGRRRGAGLGLSIVKSFVELHGGSVRIETGESRGTTVTCIFPARPAGLRAAAE
                     "
     gene            complement(6465..>7409)
                     /locus_tag="VGM46_02550"
     CDS             complement(6465..>7409)
                     /locus_tag="VGM46_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008935052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEY4191502.1"
                     /translation="LSNPQSEEEEYFVAQIKKRMKETPGFFTRQRDAIKGVTEETTTG
                     VNRLYQLQKKGLLPFPAINVNDSVTKSKFDNKYGCKESLVDGIRRGTDVMMAGKVAVV
                     CGYGDVGKGSSASLKGAGARVKVTEVDPICALQAAMDGFEVVTLEDAAPTADIVITTT
                     GNKDVITLDHMRAMKDMVIVGNIGHFDNEIQVAALRNLKWTNVKPQVDMISFPDGKRM
                     ILLSEGRLLNLGNATGHPSFVMSASFSNQVLAQIELFTKPGQYENQVYVLPKHLDEKV
                     ARLHLDKLGAKLTTLSGEQAAYIGVTPQGPFKAEHYRY"
BASE COUNT         1315 a         2474 c         2340 g         1280 t
ORIGIN      
        1 ggcagcgtgg cgatatccgg gcggccggtg ccgccgcgaa gcgcgaccag ttcgatcact
       61 tccgccgcgt ggcgaacgcc cggccgttcc agtccgaccg tcagcagcgg atgcttgagc
      121 aaggcaagca ggccaaccgg atcacccggc ccgaaggccg cgccaagcgt cagccgaagc
      181 aacccggccg gcggcgtctg gctgagcggc gttccgccgg aatcatcggc gacgataccg
      241 aagcgcagca attccgccga aacccgccgc gccagggcgc ggtcgccggt gaccagcgcc
      301 gcgcgccggc ccttctgttc cacggcgtgc cggagcgcga tggcgatggc ggcggcctcg
      361 tcgcgttcat tggccgcttc cagcagcgtg acgccggcaa aggccgcgtc gacatcgctg
      421 gatgcgaact ggcttcgcgt ctcggcccag cgttccgtgg tttccgccgg ccgcagcgct
      481 tctccggtca acgccgtgcg caaggccagc ggatcgtcac cgatctgctc gacatcgcgc
      541 cgcacgatgc cgatcttggc gatcagcttg gcaaggccat attgcggatg gccaagcaag
      601 gccggccggg cgtctttcgc cgagatcgcg gcaaacgagg cgtcgtccag caccttgtcg
      661 aggccgggca gcacgaccgc gcccagcggc aggccggcaa tcaccgacag gagttcggcg
      721 gtggccggaa tggagccggt ggagccggcg gcgatgaccg gcccggcggg cggattgcgc
      781 ttcagccgcg ccgcttccag ccggatcagc gcgctgcgat gcgcggcggg gttcgaccgg
      841 tcgagttcgg ccagcagcgt cggccagttc tcggtgacga tcttgaggaa atccagcgtc
      901 acctgccacc agccggcaag gttcccggtc accagatcgg ccagcctggt ccagtcggag
      961 ccttccgtct cgatctcgtc catcagctgc gccaggtcgc gcgccagcca gatggcgtcg
     1021 gcggttgacg ccggcaccac catttcttcc tcgaacaggg cggcgacatg ggcaggcagg
     1081 cggcgcttcc aggcgcgcac cagcggcgcc agcagcaaca gccgctccag cgcgggaata
     1141 ggtggcgcaa ggtcgagcgc ggcagcgctt tcaggcgctt gcgcctcgaa cagcgcttca
     1201 tcctcgtcga attcgccaag cggccggatg accggcagga tggcagagcc gccgcccatc
     1261 agttcgacca gttcgccccg cagcgcgcgc gccgcacggc gcgtcggcac gtagatggtc
     1321 acattggcca atgccagcgg atcgccatcg aagcgaaagc cgggaatgag cgtaccggaa
     1381 agcagcgcct tcgccagcgt cggcaggaaa ggcgcaccgg gcgcgatggt caggacgcgg
     1441 cgcacatcgc tcatggcgtt cccgaaagag cgttcgccac cgccgcctcg gcaagcggga
     1501 tcgcctccgg cgtgccgacg gtgatccagc ttccatgcat cgccatgccg aacaggcgtc
     1561 cggccttaat ggccgcatcg aaataacggt tcagcgacag cggcccgaca ggcgcattct
     1621 tgaaaatgcg cggatgcatg atcgccgcgc cggcatagat cagtcccgtc gcgccgcctt
     1681 gcgcacggcg cagggcgccg cccggcgaca tcaggaagtc ggtgccgccg ctatggccgg
     1741 tcgccgaggc gatatctgca agcatcagca gaatatccgt cttcgcatcg tcccaggcaa
     1801 gggcaagacg gtcgaggctg ggcgcgccgc tgtcgatcca gaacgtatcg gcattgacga
     1861 cgtagaaagg ctcttgcccc agtcgcggca gcgccttgac gatgccgccg gcagaatcca
     1921 gcaggccgtc gctctcgtcg gagatgacga tcttcggcgc ggatcggcag aaggtatgcg
     1981 cgacgatctg ctccgggaaa tagtggacat tgacgaccgc cttttcgaca ccggccgcag
     2041 ccaggctgtc cagcccccag tcgagcaacg tcttgcccgc gaccctgacc agcggcttcg
     2101 gcatcgtctc ggtgatcggc cgcatccgcc tgccgagacc ggcggcaagc accatggctg
     2161 tcttgggagc ggatgcggtc acagcgttct ttcttccagc aatccgtgct cgacgtagaa
     2221 ttcacgcaag ctggccaggg cgggatgccc gagcgcccga cgcagatagt cgcgaatgcg
     2281 cggcaaatgt tttagatatt ccggcttgcc gtcacgccgg ttcaaccgca cgaaaatgcc
     2341 caatatcttc gaattgcgct gggccgccat gatggcatag gcttcgcgga acgcctcttc
     2401 gtcgaacccg ccggcgccac gacgcgccgc cacataggca tcgagcgcag ccttttctgt
     2461 ctccggcggt atggtgacgc gggcgtccat ggcaagcgat gcgacgtcat acgcggcagg
     2521 cccgaccagc gcatcttgaa agtccacgat gccgagccgg tcatggcctt gccgttcacc
     2581 ccgccagata atgttgggcg aatgaaaatc gcgcagcatc agcgtatatt cgctgcctga
     2641 aagcctggcg aaaagcgcat cccattcgcg ctgatagccc tggcgcagtt cctccgacgc
     2701 cggcctgccg gaaacggcgg gcacatacca gtctaccaga agctcggcct cgatcatcat
     2761 cgcgtcgcgg tcgaagggcg gcaccgcgtg aacgacgccc accgctgcct ccaggcgata
     2821 gtgccatggc cgcgagtgca tcatcgcaag cagttccgcc gccgcggcat agcgcgcatt
     2881 aaccggctgg ccatcggcat cgaggaagct ttcggaaccg agatggctta gcagcaggaa
     2941 accctgcgcc agatcctgcg catggatttc aggcacgctg acaccggccg aagcaagcgc
     3001 gcggtcgatg gcgacgaagg ccgaaacggt gcgcgaggtg tgagcgatca gcgcataggg
     3061 cttgccatcg cgcaccggcg gccccagcat cagttcaggc gaattcatca gcacgcgcgg
     3121 cgaaaagcct gacagcgtcg cgatctcata ggagcgggcc gaggcatccg cgacgaagcg
     3181 gcggcgctgc gccgtgcccc agccggcgtc ctccaaaaag ccgcgcatag ccagcgaacg
     3241 cgccatccgc tcgaacgccg cgccctcgcc cgacagccgg gcgatgcgcc cctcgccgtc
     3301 ctgctccaac tcgaccaaga ccgtcgttgc cggcagcctg gcctcggcgc ggtccggcca
     3361 ttcgaccagc gccgcgccat cggccagcgc ttcatcgaag cccaactcat ccagttcctg
     3421 tgccgacgac aggcgataga gatcgaaatg atgcaccgga ggccgcacat cgtagctctg
     3481 gacgagcgtg aaggtcgggc ttggcacctc cagcgccgcg tcatcggcaa gcgctcggat
     3541 cagcgcccgc gccaaagtcg atttgccggc gccgagatcg cccgaaagcg ccagtacatc
     3601 gccgggccgc agcgccatgg cgaggtcttc gccaagctgc gcggttgcgg cttcatcggc
     3661 gagaaaacgc ttcagcacgg acttcgccat gcgcgacgct attcggctgc ggcgcgcagg
     3721 ccggccggcc gggccgggaa gatgcaggtg acagtcgtgc cccggctttc gccggtttcg
     3781 atgcgcacgc taccgccatg cagctcgacg aagctcttga cgatggacag gccgagaccc
     3841 gcaccacgcc gcctgccgcc gttgacgcgc ggctcgaagc gccggaacac cgcatccagc
     3901 acgtcttgcg gcatgccggg accgtcatca tgcacggaga actccacgcc atccgcagac
     3961 tgccggcagg caagcttgat gacgcctgct tccggcgcgt aattggcggc attgctcagt
     4021 agattgtaaa ggatctgccg gacgcgcgtt tcatcgccat ggaagctttt cggcgcactg
     4081 ctggcgtcga tctcgagcct gatcgagtgt tcgtctagcc ggtccgccac cagttcggcg
     4141 gccgccgcaa tggtcctgtc caccggcacc tcggaaattt cgagctgcat gatgccggca
     4201 tcgacggtgg caaggtcgag aatgtcgttg acgatcgtga gcagcaccga tgaagacgag
     4261 ccgacatgct cgacatagtc gcgctggcgc ggcgtcaacg gccctgtcgc cggctgcgac
     4321 agaagctcgg tgaagccgat aatattggtc agcggcgaac gtagttcata ggagacatgc
     4381 tgcacgaaat cgttcttgag ctggtcggat ttgagaagcg cctcgttctt gtccttcagc
     4441 gcgcgttcaa cattcacgct gtctgtgacg tcgacgaagg tcatcatcac ctgcccgttc
     4501 ggcagatgga tgacggcata gcgcaggatg ctgccattgg tcagctcggt ctggccatga
     4561 cggtcgcgcc gttcctcgtc gaagccggtg atcgtggctg cgaattcgcc ccatgggctg
     4621 tcgacggcgc ggctgtcgcc cagcgcccgg atggcggaga catggacgct tggcttgacc
     4681 gcgccgctgt ccagccccca cagcgtggcg aacgccggat tggacaggcg gagccggccg
     4741 tccgggccga acaccgccac gccctcagcc agattgtcga gcgtctcgcc ctgcacgcgc
     4801 accgccgtct ggtaacggct ttccagatcg atcttttcgg tcaggttctc gaacacccag
     4861 gtaacgccgc ccttgggctg cggattggcg acgacccgga tggtcttgcc gtcgggcaga
     4921 tgccaccaat gttcctgcgg ctcgaccgcg cgataggcgc aaagcaggcc ttccttccag
     4981 cgccgccatt ccggctgctc ggcgatcttt ccttcgctgc gcagcctgtc cagaaatagc
     5041 gcgttgtcgg gcgcgctatg caggaaaccg ctgtcaaggc cccacaattt ctggaaggct
     5101 tggttgaaga aacgcagttt ttcgtccgtg tcgaaggtcg ccaccgccgt attgagctga
     5161 tccagcgtat cggcatggct gcgcatcgtg cgctcatatt cggcccggat cgtctccatg
     5221 gcgctggtgt cggaggcgag gccggcggag ccgaaaggcc cggcgaaatc cgtcaccgca
     5281 tatttgcgcc tgtcgccttc gataacggtc gacagcgtct gctcgaacac cgggcgggcc
     5341 cgatgctgct cgacaattgc ctcgcgcgcc tggccgccca gaaattccct ggcgtcgcgc
     5401 acggccgcgt cgacgtcttc cgcctcgacc gcggccgcat aggcgcggtt cacccatttc
     5461 agccgcccat cggcggcgcg catccaaaca ggcatccgca gcgcgctgag caggccgatc
     5521 atggtttcgt ggttttcgac aagctgctgg ttttccagct tcagccgcgc ctggcttcgc
     5581 tgggtttccg acagcgaggt gaagcgaacg atgatgtggg ccgaactctt gcgcccattg
     5641 acgtcgagcg gcatgccgct ctgcgtctcg gcggtgaaat caaacggtgc gcccttttcg
     5701 cgcaacgccg cgatggcatg ttcgagcatc gcggccgaac gcggcatcag ccagcgcccg
     5761 aaagccagca aggcagctct ctcctccggc acgccgctat cgatcggcag cgcgcctatc
     5821 agttcaggct tcttgttttc cggaccccag acgatgacgc gctggtcgcg caggttgagc
     5881 atggcgtccg cacgctgcaa gccggcattg gcgtcggaca agcgcgtctt gaggttgacg
     5941 ttctcgccgg cgatgcggcc tcgctcacgg atgaggacga cagccgaaat cagtgcgccg
     6001 gccatgacgc cgacgaacac cgccagttgc atgacatcga ccgtgctgat gggcagcgcc
     6061 gaccctttcg agacgccctc cgcgtaggca atccccccaa aggcaattcc ggcaggaagc
     6121 gccaaggcca gggccgcgcc cgcccgcagc gccatggtcc gtgcccggtg ccagcccgcg
     6181 ccgcaccggc tgccggattt cccggccatc gaatcgttac ggccgccgga aacggccttt
     6241 cccgcgtgaa gcggggtttt ccccggcatg tcctggtcct ctccgccgcc tgaatgcaag
     6301 actgccctga tcgcgccagt cccggccccg tccggccgcc acaagcgaat cacatcacca
     6361 taaactctcg ccgaatcgcc gtgaagcgtg ttctcgcgca aaaataaaag ccgggcgaat
     6421 gtcaatcgcc cggcttcaat atcttgtggg agcttacgct atggttaata gcggtagtgt
     6481 tcagccttga acggaccttg cggagtgacg ccgatataag cggcctgttc gccgctaagc
     6541 gtggtcaact tggctccaag cttgtcgaga tgcaggcggg ccaccttttc atcgagatgc
     6601 ttgggcagca catagacctg gttttcatac tggccgggct tggtgaacag ctcgatctgc
     6661 gccagcacct ggttgctgaa cgatgccgac atgacgaagc tcggatggcc ggtggcgttg
     6721 ccgaggttga gcaggcggcc ttccgacaga aggatcatgc gcttgccgtc cgggaacgag
     6781 atcatgtcga cctgcggctt gacgttggtc catttcaggt tgcgcagcgc cgcgacctga
     6841 atctcgttgt cgaaatggcc gatattgccg acgatcacca tgtccttcat cgcccgcata
     6901 tggtcgagcg tgatgacgtc cttgttgccg gtggtggtga tgacgatatc ggccgtcggc
     6961 gcggcgtctt ccagcgtaac cacttcgaag ccgtccatcg ccgcctgaag cgcacagatc
     7021 ggatcgactt ccgtgacctt gacgcgggcg ccagcgccct tgagcgaagc cgacgaaccc
     7081 ttgccgacat cgccatagcc gcagacgacg gcgaccttgc cggccatcat cacatcggtg
     7141 ccgcgacgaa tgccgtcgac cagtgattcc ttgcagccat atttgttgtc gaacttcgac
     7201 ttggtgacgg aatcgttgac gttgatggcc gggaagggca gcaggccctt cttctgcaac
     7261 tgatagagcc ggttgacgcc cgtagtcgtc tcttcggtaa cgcccttgat ggcatccctc
     7321 tgcctggtga agaagccggg cgtttccttc atgcgcttct tgatctgcgc gacgaaatat
     7381 tcctcttcct cgctctgcgg gttggacag
//
LOCUS       DASYCS010000070         2466 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1900362,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000070 DASYCS010000000
VERSION     DASYCS010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1900362"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /gene="ybgF"
                     /locus_tag="VGM46_02555"
     CDS             <1..521
                     /gene="ybgF"
                     /locus_tag="VGM46_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HEY4191503.1"
                     /translation="VDKNGNVVDAGGSTQSTTPMEQAPAAAQAQGNKSANTVVAALPP
                     ADSPDELYRNSYQFILSGDYGTAEQGFRDHIARFPKDAKAADAHYWLGESLLGQQKYR
                     DAAEIFLAASKDYPKAKKAPDMLLKLGVSLVGLKQRDVACATFGEIAKRYPDVSGALK
                     ERIKQERAQASC"
     gene            515..1915
                     /gene="tilS"
                     /locus_tag="VGM46_02560"
     CDS             515..1915
                     /gene="tilS"
                     /locus_tag="VGM46_02560"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006200110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEY4191504.1"
                     /translation="MLTSPLADDGAAAAPGLSDTVFSTIDFTNGALVAISGGGDSTAL
                     LLLLKNYLDRHAPRAPLLAVTVDHGLRADSAAEARAVSRLCAEKGIDHKTMLWEGDKP
                     STGLPAAAREARYRLLAQAAGEAGVRLILTGHTADDQAETVLMRQERADTASPHGRGL
                     AGMAAATLYDWDIWIVRPLLGAKRQALREFLRREKMGWIDDPTNQDETFERPRIRAAL
                     KDRGRSFVEATALAMQEAEIRRNASRRAAAIVRAFASRPAPGLIRLDPAFAAGDRSAA
                     IAALRVLLATIGGTAFPPGDARTAALFDKMAAAAPFRATLSRTVIDKRRAAIFLHREL
                     RDLPTAASAVDGMVWDGRRRIDVGDEANGLLVEPPALRAAAADMDYAGNAPASLLRAA
                     SAAEPCFRRDLECSAAANDRRPGVRISPVIAPYARYLPLFDFELAGAVAELIGAPPLP
                     SPPFRGFDASRSLAKA"
     gene            2021..>2466
                     /locus_tag="VGM46_02565"
     CDS             2021..>2466
                     /locus_tag="VGM46_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HEY4191505.1"
                     /translation="MNPNYRNLALWAIIAVLLIALFNLFQTPQTRGASSDIPYSQFLQ
                     EVDAGRVKSVTIAGERISGSYTDNSTGFQTYSPGDQSLVSRLESKQVSINARPETDGS
                     NTLLGYLISWLPMILILGVWIFFMRQMQSGSGRAMGFGKSKAKLLT"
BASE COUNT          425 a          878 c          745 g          418 t
ORIGIN      
        1 ccgtcgacaa gaacggcaat gtggtcgatg cgggcggcag cacgcaatcg accacgccga
       61 tggaacaggc cccggcggcg gcgcaagccc aagggaacaa atccgcaaac accgtcgttg
      121 ccgcgctgcc gcccgccgac agccccgacg agctttatcg caactcctac cagttcattc
      181 tttcgggcga ctacggcacc gccgagcagg gcttccgcga ccacatcgcc cgcttcccca
      241 aggatgccaa ggccgccgac gcccactact ggctgggcga atcgctgctt ggccagcaga
      301 aataccggga tgccgccgaa atcttccttg ccgccagcaa ggattatccg aaggccaaga
      361 aggccccgga catgctgctg aaactcggcg tttcgcttgt cggcctcaag caacgcgacg
      421 tcgcctgcgc caccttcggc gagatcgcca agcgctatcc cgacgtttcc ggcgcgctca
      481 aggaacgcat caagcaggaa cgggcccagg cttcgtgctg acatcgcctc tggcggatga
      541 cggcgctgcc gccgctcccg gcctttccga cacggttttt tccaccatcg attttaccaa
      601 tggcgctttg gtcgccatct ccggcggcgg cgattccacc gcgcttttat tgcttctcaa
      661 gaattatctg gaccggcacg cgccccgcgc cccgcttctg gcggtgacgg tcgatcacgg
      721 cctgcgcgct gattccgccg ccgaagcgcg cgccgtaagt cgcctctgcg cggaaaaagg
      781 catcgaccac aaaaccatgt tatgggaagg cgacaagcct tcgaccggcc ttcccgccgc
      841 cgccagggaa gcccgctacc gcctgcttgc ccaggctgcc ggcgaagccg gcgtcaggct
      901 gatccttacc ggccataccg ccgacgacca ggccgaaacc gtgctgatgc gacaggagcg
      961 ggcggatacc gcatcgccgc acgggcgcgg acttgccggc atggccgccg ccacgctcta
     1021 cgactgggat atctggattg tgcgcccgct gctcggcgcg aagcggcagg cgctgcgtga
     1081 attcctgcgg cgcgagaaaa tgggctggat cgacgatccg accaaccagg acgagacgtt
     1141 cgagcgaccg cgcatccgcg ccgccctcaa ggatcgcggc cgatccttcg tcgaagcaac
     1201 cgcgcttgcc atgcaagagg ccgaaatccg ccggaacgca agccgccggg cagcggcgat
     1261 cgtgcgcgcc ttcgccagcc ggccggctcc gggattgatc cgcctcgacc ccgcctttgc
     1321 cgccggcgac cgcagtgccg ccattgccgc cctgcgcgtt ttgctggcca ccatcggcgg
     1381 aacggccttc ccgcccggcg acgcccgcac ggcagccctt ttcgataaaa tggcggcggc
     1441 ggcgccgttt cgcgccacat tgtcgcggac ggtaatcgac aaaaggcgcg ccgccatttt
     1501 cctccaccgg gaattgcgcg atctgcccac cgccgcttcg gccgtcgatg gcatggtctg
     1561 ggatggccgc cgccgcatcg atgtcggcga cgaggccaac ggcctgctgg tcgaaccgcc
     1621 ggctttgcgg gccgcggccg ccgatatgga ttatgcaggc aacgccccgg cgagcctcct
     1681 gcgcgcggca agcgctgcgg aaccgtgctt tcgacgggat ttggagtgca gcgccgccgc
     1741 gaacgacagg cgaccgggcg tgcggatttc gcccgtcatc gccccctatg cgcgctatct
     1801 gccgctgttc gacttcgagc ttgccggggc ggttgctgaa ctcatcggcg cgccgcccct
     1861 gccgtcgccg cctttccgtg gcttcgatgc aagccgatcg ctggccaaag cttaaccgag
     1921 acgaggagtt gtgcttggca aggccgggta cggtccctat gttagggcga gcttcctctt
     1981 aaacgcgcgc gtccgcctcg tggcgccaac gggacattcg atgaatccta actatcgcaa
     2041 tctggcgctc tgggcgatca tagcagtcct gctgatcgcg ctgttcaacc tgttccagac
     2101 cccgcagacg cgcggcgcgt ccagcgacat tccctattcg cagttcttgc aggaagtcga
     2161 tgccggccgc gtcaagtccg taaccatcgc cggcgagcgc atcagcggca gctacaccga
     2221 caattccacc ggcttccaga cctattcgcc cggcgaccag tcgctggtgt cgaggcttga
     2281 gtccaagcag gtcagcatca acgcgcgccc cgaaacggat ggctccaaca cgctgctcgg
     2341 ctacctgatc tcatggctgc cgatgatcct catcctcggc gtctggatat tcttcatgcg
     2401 ccagatgcag tccggctccg gccgcgccat gggcttcggc aaatccaagg ccaagcttct
     2461 gaccga
//
LOCUS       DASYCS010000071         4039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1610697,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000071 DASYCS010000000
VERSION     DASYCS010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1610697"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            225..>4039
                     /locus_tag="VGM46_02570"
     CDS             225..>4039
                     /locus_tag="VGM46_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006332395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ndvB"
                     /protein_id="HEY4191506.1"
                     /translation="MNIQPSLPQTEKTKTAKAAAPLGQASSHASFPVDAEEPIRSQFL
                     DEERLRALGESLASGDVGDLTELTPFAFQARIRDNAKKILEVYRATNAAQGRGEAVTP
                     AAQWLLDNNYLVEETIFQVKRDLPGRFYRQLPTLKLSDGTSIPRALAIAWAYVAHSDS
                     SVSANMFKAIVEGFQSIEPMKIGEIWALPSLLRFVLVENLRRLAVRVNRSRDMRQIAN
                     DVADRVLASKDDDDKAILDAYRTHAQDTTFATQLLYRLRDGSQNSGKALDWLESELET
                     SGSDAEEIIIGEHQTLSSGNVTTGNIIRGLRLINDVDWTYWFEDVSRVDALLREHTDF
                     HALDFASRDQYRQSIEDLARRSDLSEFRVAEKATELAGAVTAPGGQDSDQVEADLRLP
                     DKTDVGFFLVGPRRRELERAIGYRAGIALRLNRAYRMTGWFGIVLPVFALTVLLLALT
                     GNALQTLNILPDPAIVLMLVLFAVPASEGALGFFNTVVLLFLKPTRLVGYEFKDGLPA
                     SARTMVVVPSLIGSRDDVEENIRNIEVHHLANTSSELYFALLSDWPDSTSEVDEADIE
                     ILDFAKTQVARLNQRYPSEGVPRFHLLHRHRLYNEVQGSWMGWERKRGKLHELNLLLR
                     GDSDTTFMPLDAPLPENVVHVMTLDADTRTTRDAISRLVGKLSHPLNRPHFDAARRKI
                     TAGYAILQPRITPSLTSGDEASFFQRVFSASRGLDPYVFAVSDLYQDLFGDGSFTGKG
                     LYHVDAFEAALKDRIEENTILSHDLLEGSLAQSALVTDVELVEDYPTRYSVDASRQHR
                     WARGDWQLLGFIFSPRSEVPALSRWKMIDNLRRSLTPIFWVMAAIAGWTFLPFTQAAQ
                     WQALLILSLFMAPTFDIVNSILPKTRDATARGHFSALMHDVAFGTAMVALKIVLMAHH
                     AWMMGDAIIRTIYRLFFSRKYLLEWRTASQAQKSGGNDLASYYAMMHGGVIVGLIGLA
                     IPVLADSTGAFVAFFFALFWIGSPAFAWVISRSAETEDRLRISPHDIHALRIVARRTW
                     HYFETFVTADENHLPPDNFQETPAPVIAHRTSPTNIGVYLLSVVCARDFGWISLSDAV
                     NRIDATMKTIESMPRSRGHLFNWYDTLTLKPLYPLYISAVDSGNLAGHLIAVAATCAE
                     WAEAPSVHLQADFEGILDNVAILDESLDELPDDRRQLRPLRQRLADRLDGMRRAVQTI
                     KQQPEMASIRTINLAVLAGEIRKLAAAIHTEAASARSDVIVDWAARLEATCEAHVLDS
                     HSDDAAIAAL"
BASE COUNT          735 a         1336 c         1172 g          796 t
ORIGIN      
        1 cgcgtgccct gaaacacgaa aaagcccgaa tcctttcctc tcgctcctaa ccgagtggaa
       61 caaaagcctg tgtctgccgg ttgtcgcggc agccacggct gttattgtcg gtcggcgacg
      121 tcactccacg acttttgttc gatgctgcgg tacatggtgg tatcggcttg gaaatttgca
      181 gcagcagcga tattaccgcc gagcgctgct tcatggaatt ttgaatgaac atacaaccca
      241 gccttcctca gacggaaaaa acgaaaacgg cgaaggctgc cgccccactc ggccaagcaa
      301 gttcgcatgc gagctttccc gtcgacgcgg aagaaccgat ccgctcccag ttcctggacg
      361 aggagcggct gcgtgcgttg ggcgaaagct tggccagtgg cgatgtcggc gatctgaccg
      421 aactgacgcc cttcgcgttt caggctcgca tacgcgacaa tgccaagaag atacttgagg
      481 tttaccgggc caccaacgcg gcgcagggca ggggcgaagc ggtaaccccc gccgcgcaat
      541 ggctgctcga taacaactac ctggtcgagg aaacgatttt ccaggtcaag cgcgacctgc
      601 cgggccgctt ttatcggcaa ttgccgacgc tgaaattgtc ggacggcacc agcatcccgc
      661 gcgcgctggc catcgcctgg gcttacgtcg cccattccga cagctccgtt tcggccaata
      721 tgttcaaggc catcgtcgag ggctttcaat cgatcgagcc gatgaagatc ggcgagatat
      781 gggcgctgcc ctcgctgttg cgcttcgttc tggtggaaaa ccttcgccgg cttgcggtcc
      841 gggtcaaccg ctcgcgcgac atgcgccaga tcgccaacga tgtggccgac cgggtgcttg
      901 cctcgaaaga cgacgacgac aaggccatcc tcgacgccta ccgcacccat gcgcaggaca
      961 ccaccttcgc cacccagctg ctctaccggc tgcgcgacgg atcgcagaat tccggcaagg
     1021 ccctggattg gcttgagagc gagcttgaga catcaggctc ggacgccgag gaaatcatca
     1081 ttggcgagca ccagaccctg tcgagcggca acgtcacgac gggcaacatc atccggggcc
     1141 tgcgcctcat caacgatgtc gactggacct actggttcga ggatgtaagc cgggtcgatg
     1201 cgctgctgcg cgagcatacc gacttccatg cgctcgattt cgcatcgcgc gaccagtacc
     1261 gccaatccat cgaggacctg gcacgccgct cggacctttc ggaattccgg gtggcggaaa
     1321 aggcgacgga gcttgccggc gccgtgacgg ccccaggcgg gcaggattcg gatcaggtcg
     1381 aagccgattt gcggctgccc gacaaaaccg atgtcggctt cttcctggtc ggcccgcgcc
     1441 gccgggagct tgagcgcgcg ataggctatc gcgcgggcat cgcgttgcgg ctcaaccggg
     1501 cataccgcat gacgggatgg ttcggcatcg tgctgccggt gttcgcgctg acggtgctgt
     1561 tgctcgccct gactggtaac gccctgcaaa cgctgaatat tcttcccgat cctgccatcg
     1621 tgctgatgct ggttctgttc gccgtgcccg ccagcgaagg cgcgctcggt ttcttcaata
     1681 cggttgtatt gctgttcctg aagccgacgc ggttggtcgg ctacgaattc aaggacggtc
     1741 ttccggccag cgcacgaacg atggtggtgg tgccgtcgct gatcggctcg cgcgacgatg
     1801 tcgaggaaaa catccgcaac atcgaagtcc atcacctggc caacacatcg agcgaactct
     1861 atttcgccct gctgtcggat tggcccgaca gtacaagcga agtcgatgag gcggatatcg
     1921 agattctcga tttcgccaag acgcaggtcg ctcgcctcaa ccagcgctat ccaagcgagg
     1981 gcgtgccgcg cttccatctt ttgcaccggc accggctcta taacgaagtc cagggtagct
     2041 ggatgggctg ggagcgcaag cgcggcaagc tgcacgagct gaaccttctg ctgcgcggcg
     2101 acagcgacac caccttcatg ccgctcgatg cgccgctgcc ggaaaacgtc gtccatgtga
     2161 tgacgctgga tgccgatacc cgcaccaccc gcgatgcgat ttcgcgcctg gtcggcaagc
     2221 tttcccaccc gctcaaccgc ccgcatttcg atgccgcgcg ccgcaagatc acggccggct
     2281 atgcgatctt gcagccgcgc atcactcctt cgctgacatc gggcgacgaa gcctcattct
     2341 tccagcgcgt cttttcggcg agccgcgggc tggaccccta cgtgtttgcg gtgtccgacc
     2401 tctatcagga ccttttcggc gacggctcct tcaccggcaa gggcctgtac cacgtcgacg
     2461 cattcgaagc cgcgctcaag gaccgcatcg aggaaaacac catcctcagc cacgatctgc
     2521 tcgaaggctc cctggcgcaa tcggcgctgg ttaccgatgt tgaattggtc gaggattatc
     2581 cgacccgcta ttcggtcgat gcttcgcgcc agcatcgctg ggcgcgcggc gactggcagt
     2641 tgctgggctt catcttctct ccccgttcgg aagtgccagc cctttcgcgc tggaagatga
     2701 tcgacaatct gcgccgctcg ctgacgccca ttttctgggt tatggccgcg attgccggct
     2761 ggacgttcct gccgttcacg caggccgccc agtggcaggc gctgctgatc ctcagcctgt
     2821 tcatggcgcc caccttcgac atcgtcaatt cgatcctgcc caagacccgc gacgccaccg
     2881 cgcgcgggca tttctcggcg ttgatgcatg acgtcgcctt cggcaccgcc atggttgcgc
     2941 tgaagatcgt cctgatggcc catcacgcct ggatgatggg cgatgcgatc atccgcacga
     3001 tctatcggct gttcttcagc cgcaaatatc tgctggaatg gcgtaccgcc tcgcaggcgc
     3061 aaaaatcggg cggcaacgac ctcgcttcct attacgccat gatgcatggc ggcgtcatcg
     3121 tcggcctcat cggcctcgct attccggtgc tggccgattc caccggcgct ttcgtcgcct
     3181 tcttcttcgc cttgttctgg atcggctcac cggccttcgc ctgggtcatc agccgctcgg
     3241 ccgaaaccga ggaccggctt cgcatttcgc cgcacgacat ccatgccttg cgcatcgtcg
     3301 cgcgccgtac ctggcactat ttcgaaacgt tcgtcacggc cgacgaaaac cacctgccgc
     3361 ccgataattt ccaggagacg ccggctcccg tcatcgccca ccgcacgtca ccgaccaata
     3421 tcggcgtcta tctgctttcc gtcgtttgcg cgcgcgactt cggctggatc agcctttccg
     3481 atgccgtcaa ccgcattgac gcgacgatga agaccatcga aagcatgccg cgcagccgcg
     3541 gtcacctgtt caactggtat gacacgctga cactgaagcc gctctatccg ctttatatct
     3601 cggccgtcga cagcggcaat ttggccggcc atctcattgc cgtggccgcc acctgcgccg
     3661 aatgggccga agcgccttcg gttcatttgc aggccgattt cgaaggcatt ctcgataacg
     3721 tcgccattct cgacgagagc ctggacgaac tgccggatga ccgccgccaa ttgcgcccgc
     3781 tgcgccagcg tctggccgac aggctggatg gcatgcgccg tgcggtccag accatcaagc
     3841 aacagcctga aatggcctcg atccgcacca tcaacctggc ggtgctggcc ggcgagatac
     3901 gcaagctcgc cgctgccatc cacaccgagg cggcctcggc gcgcagcgat gtcatcgtcg
     3961 actgggcggc ccggcttgaa gcaacctgcg aagcccacgt cctggattcg cacagcgacg
     4021 acgcggcgat cgccgcgct
//
LOCUS       DASYCS010000072         1573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4365422,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000072 DASYCS010000000
VERSION     DASYCS010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4365422"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1491)
                     /locus_tag="VGM46_02575"
     CDS             complement(<1..1491)
                     /locus_tag="VGM46_02575"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY4191507.1"
                     /translation="MKFTRLRLSGFKSFVEPTELYIESGLTAVIGPNGCGKSNLFDAM
                     RWVMGETRPSSVRGSEMDDVIFSGTAGRPARNVAEVTLFVDNSEHKAGGPYAGYETIE
                     VSRRIEREAGSVYRINGRDVRQRDVQIFFADASSGAGSTAFVRQGQIGLLISQKPLAR
                     RAILEEAAGIGGLHQRRHEAELRLKAAETNLSRLEDVIREVETQLASLKRQARQASRY
                     RNLSGHIRKAEALAHFLRWAQASARATQAAEELAQAASVVADATEAAAKASTLQSDAA
                     SILPPLRQDEAEKSAAHHRLIVAREQLEAEEARAREAAQRFRQLIAQGEADITREQTL
                     DQDAAAALGTLAGERETLELAATSATSDIAAAEEQSVELKEKLTETERLLEKLTSEFS
                     DWNANKASHERSRDLAAGLTETSATQLADARVRLDRAMESAHAAPDVAAADSQTEAAR
                     AASEGARALAVQARNDRTEAEAAETTAREPLETAEREVHRLSAEVKA"
BASE COUNT          234 a          489 c          524 g          326 t
ORIGIN      
        1 tgctttcact tcggcggaga ggcgatggac ttcgcgctcg gcggtttcca gcggctcgcg
       61 tgctgtggtt tcggcagctt cggcttcggt gcggtcgttt cgggcttgta ccgccagggc
      121 gcgtgcacct tccgacgcgg cacgcgctgc ttcggtctgg ctgtcggcgg cagcgacgtc
      181 gggggccgcg tgtgcgcttt ccatggcgcg gtcgaggcgt acgcgcgcat cggcaagctg
      241 tgtcgcgctg gtttcggtca gaccggcggc aaggtcgcgg ctgcgctcat ggctggcctt
      301 gttggcattc caatccgaaa attcggaggt aagcttttcc agaagccttt cggtttcggt
      361 cagcttttct ttgagttcga ccgattgttc ttcggccgcg gcaatgtcgg aagtggcact
      421 ggtggcggcg agttcgagcg tttcgcgctc gccggccagc gtccccaacg cggctgcggc
      481 atcctggtcg agggtctgct cgcgcgtgat gtcggcttcg ccctgcgcga tcagttgccg
      541 gaaacgctgc gcggcctcgc gcgcacgagc ttcttccgct tcgagctgtt cgcgcgccac
      601 gatgaggcga tgatgggcgg cggacttctc cgcttcatcc tgacggagcg gcggcaaaat
      661 ggatgcggcg tcgctctgga gggtcgaagc ctttgccgca gcctcggtcg catccgctac
      721 gacggatgcc gcttgcgcca attcttccgc cgcctgagtc gcgcgcgccg atgcctgggc
      781 ccagcgcagg aaatgcgcca gcgcttcggc cttgcggata tggccagaga gattgcggta
      841 acgcgacgcc tggcgcgcct gacgtttcag actggcaagc tgcgtttcca cttcccggat
      901 aacgtcttcc aggcgggaca ggttggtctc cgccgctttc agccgaagct cggcttcgtg
      961 ccggcgctga tgcaggccgc cgatgccggc ggcttcctcc aggatggcgc gccgcgccag
     1021 cggcttctgg ctgatgagaa ggccgatctg gccctggcgg acaaaagcgg tcgagcctgc
     1081 gcccgaagac gcatcggcaa agaatatctg cacatcgcgc tggcgcacat cgcggccatt
     1141 gatgcgatag acgctgccgg cttcgcgctc gatgcggcga gagacttcga tcgtctcata
     1201 cccggcatag ggaccgccgg ccttgtgctc gctgttgtcg acgaaaagcg tgacttccgc
     1261 gacattgcga gccgggcggc ccgcggtgcc ggaaaaaatc acgtcgtcca tttcactgcc
     1321 gcgcacggag gagggcctgg tctcgcccat cacccagcgc atggcgtcga acaggttgga
     1381 tttgccgcag ccattcggtc cgatcaccgc cgtgaggccg gactcgatat acagctccgt
     1441 cggttcgacg aaggatttga aaccggaaag tctaagccgg gtgaacttca agaaaaacgc
     1501 gccgccttac gcttgttgaa ttgcgccggc cgccaatcat tttttcttgg cgagcgcggc
     1561 gtcgaggatt ttt
//
LOCUS       DASYCS010000073         4432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3929617,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000073 DASYCS010000000
VERSION     DASYCS010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4432
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3929617"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1443)
                     /locus_tag="VGM46_02580"
     CDS             complement(<1..1443)
                     /locus_tag="VGM46_02580"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEY4191508.1"
                     /translation="MTLPATLLEIENLSLSIRDAPILKGIDLTVAAGEVVGLVGESGS
                     GKSMTALTVMQLLPYAAHATGRIGFDGIDILNAGEDRMCTLRGNRIGMVFQEPMTALN
                     PVKTIGEQVAEGIRWHAKVSRANAEARARAILDRVGLPEAKFPLSRYPHELSGGQRQR
                     VAIAIACALQPRLLIADEPTTALDVVLQAQILDLLRELVAESRMGLLLISHDLAVVTE
                     MADRIAILRQGEIMEEGETARVLSAQVHPYTRQLARASTHVPNRHAPTLPASSGKPLL
                     SVEKITRDYPGRRLSLFKRAASVRAVDDISFSMQAGQSVALVGRSGCGKSTLARMILA
                     LDRPTAGNISFCGEQITNRSETELKPARRNMQVVFQDPYGSFDPRQKAGTLVAEPLHV
                     LENRPSPAERREMAEHALHEVGLAPSDMDKYAHEFSGGQRQRLSIARAIITRPKLIVA
                     DEPVSALDVSIRAQILDLFAELNGKLGIAYL"
     gene            complement(1440..2309)
                     /locus_tag="VGM46_02585"
     CDS             complement(1440..2309)
                     /locus_tag="VGM46_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006335434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4191509.1"
                     /translation="MTERGRQNRQIEQSLAARAFSNPSFLAGFVIALLVAAAALVSYL
                     WTPYDVTRLLIADKTQAPSPAHWFGTDHFGRDILSMVMVGARNSIAVALVAVGIGMGI
                     GVPLGLWAAARGGLPDEALMRLNDLVFAFPALLSAIMITAIFGPGAVNAIIAIGIFNI
                     PVFARVARAGGLAIWPREFILAARAAGKSKTQITLEHVLPNLATLLLVQGTIQFALGI
                     LAEAGLSYVGLGAQPPMPSWGRMLFDAQTRMVAAPWMAVFPGMAIVVTVLGLNLLGDG
                     LADILDPKSRRRR"
     gene            complement(2306..3253)
                     /locus_tag="VGM46_02590"
     CDS             complement(2306..3253)
                     /locus_tag="VGM46_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4191510.1"
                     /translation="MTGYFLKRLAISLATLIFASMVVFAVLEIIPGDPARLMLGMNAS
                     ADQLALLRDQMGLNAPVAIRYLRWVGGMLTLDFGHSYTYSVPVIDLVAERAAVSLPLA
                     LISLVLTTLIALPVGLYSASRRGHAGDTLSMGAAQLGVAVPNFWFALMLIYLFAVWLR
                     LVPAGGFPGWGAGIWPALKALILPAISLALPEAAILSRVARSALIEVLDEDYIRTAQA
                     KGMPRRAVLWRHALRNAMIPVVTIMGLQFAYLLAGTIIIENVFYLPGLGRLVFQAITQ
                     RDLVVVESVVMLLVATVIVVNLLVDLSYAIVDPRLRSRQ"
     gene            complement(3359..>4432)
                     /locus_tag="VGM46_02595"
     CDS             complement(3359..>4432)
                     /locus_tag="VGM46_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY4191511.1"
                     /translation="VVDPATVKITLKQPQGSFLYNMGWGDAVMVAPESAATNKEKPIG
                     TGPFKFQNWAKGSSITLVKADHYWGSPAFLDKAEFRIIPDAAAAVPALLSGDVQAFPF
                     FPADAVGQVKDNPALKVAIGSTEGETILAINNKKPPFDNLKVRQAISYALDRQAIIDG
                     ASGGIGAPIGSHMSPSNKAYVDLTGEYPHDPAKARELLKEAGFEGGIKATLKLPPVDY
                     ARLGGEIIASQLRDVGINLEIVPVEWAQWLDQVFTRKDYDLTIVSHTEPNDIDIYSRK
                     DYYFNYDNPAFDKVIAELNLTSDETNRNALLGQAQKILADDAVVGFLFELPKIGVWDA
                     KLQGMWVNDPIQSTDLTKVKWQD"
BASE COUNT          882 a         1326 c         1450 g          774 t
ORIGIN      
        1 tagataggcg atgccaagct tgccgttcag ttcggcgaac aggtcgagga tttgggcgcg
       61 aatggagaca tcgagcgccg agacgggttc gtcggcgacg atcagcttcg gccgggttat
      121 gatggcgcgg gcaatcgaca gacgctggcg ttgcccgccg gaaaactcat gcgcatattt
      181 gtccatgtcg gatggcgcga ggccgacttc gtgcagcgca tgttccgcca tctcgcggcg
      241 ctccgccgga gaaggcctgt tttcaagcac atgcaagggt tctgccacca gcgttccggc
      301 tttctggcgc ggatcgaacg aaccataggg gtcttgaaac accacctgca tgtttcggcg
      361 cgccggcttc agttccgttt cgctgcgatt ggttatctgc tcgccgcaaa aggagatatt
      421 gccggcggtc ggcctgtcga gcgccaggat catgcgagcc agcgtggatt ttccgcagcc
      481 ggagcgccca acgagggcaa cggactgccc ggcttgcatg gagaacgaaa tatcgtcaac
      541 ggcgcgaacc gaagcagcgc gcttgaacaa tgaaaggcgg cggccgggat agtcgcgggt
      601 aattttttcg acgctgagca gcggcttgcc ggaggaagcg ggcagggtgg gagcgtggcg
      661 gttcggcaca tgcgtcgaag cgcgcgccaa ttgccgtgta tagggatgga cctgcgccga
      721 cagaacacgc gccgtctcgc cctcttccat tatttcgccc tggcgcagga tggcgatgcg
      781 gtcggccatt tcggtgacga ccgcgagatc gtgcgaaata agcagcaggc ccatgcggct
      841 ttccgcgaca agttcgcgca acaggtcgag gatctgggct tgcagcacca catccagcgc
      901 ggtcgtcggc tcgtcggcta tgagcagtct cggttgcagc gcgcaggcga tggcgatggc
      961 aacgcgctgg cgctggccgc ccgacagttc gtgcgggtag cgcgacagcg ggaattttgc
     1021 ctcgggcaat ccgacacggt caagtatcgc gcgcgcccgc gcctcggcgt tcgccctcga
     1081 caccttggcg tgccagcgga taccttcggc cacctgttca ccgatggtct tgaccgggtt
     1141 cagcgccgtc atcggctctt gaaacaccat gccgatccgg ttgccgcgca gcgtacacat
     1201 gcggtcttcg ccggcgttca aaatatcgat gccgtcaaaa ccgatccggc ctgtggcatg
     1261 tgcggcatag ggcagaagtt gcatgaccgt cagcgcggtc atcgactttc ccgagccgga
     1321 ttcgccgacc agcccgacca cctctcccgc cgccacggta aggtcgatgc ccttgagtat
     1381 gggcgcgtcg cggatcgaca gcgacaggtt ctcgatttcg agcagcgttg cgggcagggt
     1441 catcgccgcc gcctcgattt cgggtccaga atatcggcaa ggccgtcacc gagcaggttc
     1501 agccccagca ccgtcacgac aatggccatg cccggaaaca ccgccatcca cggagcggcg
     1561 accatgcgcg tttgcgcgtc gaacagcatt cggccccagc tcggcatcgg cggttgggcg
     1621 ccgaggccga cataggacag gccggcctcg gccagaatgc caagcgcgaa ctggatggtg
     1681 ccttgaacca gaagcagcgt ggcaaggttg ggcaggacgt gttcgagcgt gatctgcgtc
     1741 ttgctcttgc cggcagcgcg agccgccagg atgaattcgc gcggccagat ggcaagcccg
     1801 ccggcgcgcg ccacacgcgc gaaaaccgga atattgaaaa taccgatggc gatgatggca
     1861 ttgaccgcgc ccggaccgaa gatggcggta atcatgattg ccgaaagcaa tgcgggaaag
     1921 gcgaagacga gatcgttcag ccgcatcagc gcttcgtccg gcaggccgcc acgcgccgcc
     1981 gcccaaaggc cgagcggaac gccgatgccc atgccgatgc cgaccgccac aagggcaacc
     2041 gcgatggaat tgcgcgcgcc gaccatgacc atcgacagga tatcgcggcc gaaatggtcg
     2101 gtgccgaacc aatgggcggg cgaaggcgcc tgcgtcttgt cggcgatgag cagcctggtc
     2161 acgtcgtaag gcgtccacag ataggaaacg agggcagccg cagccaccag cagggcgatg
     2221 acgaagccgg caaggaatga agggttggaa aaggcccttg ccgcaaggct ctgttcgatc
     2281 tgcctgtttt gtcgtccgcg ttcggtcatt gtcggcttct caggcgtggg tcgacgatcg
     2341 cgtaggagag atcgacgaga aggttcacaa cgatgacggt ggcgacgagc agcatgacga
     2401 cgctctcgac cacgaccaga tcgcgctggg ttatcgcctg aaacaccagc cgtccaaggc
     2461 ccggtaggta aaatacgttt tcgatgatga tggtgccggc cagcaggtag gcgaattgca
     2521 ggcccatgat ggtcacgacc gggatcatcg cgttgcgaag ggcgtgacgc cacaacacgg
     2581 cgcggcgcgg catgcccttg gcctgggcgg tgcggatgta atcctcgtcc agcacctcta
     2641 tcagggcgga gcgggcgacg cgcgacagga ttgcagcttc cggcaacgcc agggaaatgg
     2701 ctggtaggat cagtgctttc aaggccggcc agatgccggc gccccagccg ggaaagccgc
     2761 ccgccggcac cagccgtagc cagaccgcga ataggtagat cagcatcagc gcgaaccaga
     2821 aattgggaac ggcgacgccc agttgcgcgg cacccatgga aagcgtgtcg ccggcatggc
     2881 cgcgccggct ggccgaatag aggccgaccg gaagcgcgat gagggtggtc agcacaagcg
     2941 aaatcagcgc cagcggcagc gagacggcgg cgcgttcggc taccaggtcg ataacgggaa
     3001 cggaataggt gtaggagtgg ccgaaatcca gcgtcagcat gccgcccacc cagcgcaggt
     3061 agcggatggc gaccggagca ttgaggccca tctggtcgcg cagcagggca agctggtcgg
     3121 cgctggcatt catgcccagc atcagccgtg ccgggtcgcc gggaatgatt tccagcaccg
     3181 cgaagaccac catcgacgcg aagatgagcg ttgcaagtga gatggccaga cgcttgagaa
     3241 aataaccggt catggcaacc gcaggaaaac cgccgaagcc agcgcccggc gatcgatccc
     3301 gcccgttacg gcgatgggaa atccaggaag gctcaaggcc acaacgctcc cggctcggtc
     3361 aatcctgcca ctttaccttg gtaaggtcgg tggactggat aggatcgttc acccacatgc
     3421 cctgcaattt ggcgtcccat acaccgatct taggcagttc gaacagaaag ccgaccacgg
     3481 cgtcgtcggc caggattttc tgggcctgtc cgagcaacgc gttgcggttg gtttcgtcgg
     3541 aagtgaggtt caactccgca atcaccttgt cgaaggccgg attgtcgtaa ttgaaataat
     3601 agtccttgcg cgaataaata tcgatgtcgt tcggctcggt atgcgaaacg atggtcaggt
     3661 cgtaatcctt cctggtgaag acctgatcca gccactgcgc ccattcgacc ggaacgattt
     3721 ccagatttat cccgacgtcg cgcagttgcg aagcgatgat ctcgccgcca aggcgcgcat
     3781 aatcgaccgg gggcagcttc agcgtcgcct tgatgccgcc ctcgaaaccg gcttccttca
     3841 acaattcccg ggccttggcg gggtcgtgcg gatattcgcc ggtcaggtcg acataggcct
     3901 tgttggacgg agacatgtgc gagccgatgg gcgcgccgat gccgcctgac gcgccgtcga
     3961 tgatcgcctg gcggtcgagc gcataggaaa ttgcctgcct gaccttgaga ttgtcgaagg
     4021 gcggcttctt gttgttgatg gcgaggatcg tttcgccctc ggtcgagccg atcgcgactt
     4081 tcagggcggg attgtccttc acctggccaa ccgcgtcggc cgggaagaac gggaacgcct
     4141 gcacatcgcc ggaaagcagc gccggcacgg cggcggcggc atcggggatg atgcggaact
     4201 cggccttgtc caggaatgcc gggctgcccc agtagtggtc ggctttgacc agcgtgatcg
     4261 atgaaccctt ggcccagttc tggaacttga acggaccggt gccgatgggt ttttccttgt
     4321 tggtggcggc cgattccggc gcaaccatca ccgcatcgcc ccaacccata ttgtaaagga
     4381 aggagccttg cggctgcttc agcgtgatct tcaccgtggc cggatcgacc ac
//
LOCUS       DASYCS010000074         9289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1900750,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000074 DASYCS010000000
VERSION     DASYCS010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 9289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9289
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1900750"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /locus_tag="VGM46_02600"
     CDS             <1..982
                     /locus_tag="VGM46_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY4191512.1"
                     /translation="SRVCPSEKGTAAKLVCPYHQWTYELDGRLLFARQMAEGFDKADF
                     GLKPIACESVGGYLFICLADRPADFALVRAMIEPYLAPHRLTEAKVAFESTIIEKGNW
                     KLVWENNRECYHCAANHPELCKTFPEAPTVTGVQGANSDPEMLAHWTKCEAAGLPSAF
                     RIDPDGQYRATRAPLLRDAVSYTMTGKRAVKKNLSDTVTTEGIGSLLLYHYPTTWNHI
                     LGDHAVTFRVLPISATETAVTTKWLVHKDAVEGVDYDLAELTHVWTETNDQDRRIVEE
                     NAFGTLSPAYEPGPYSEMHEGGVIQFVNWYADFIGPRLAQGGKPALKNVA"
     gene            994..2097
                     /locus_tag="VGM46_02605"
     CDS             994..2097
                     /locus_tag="VGM46_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018238993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid-cluster NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY4191513.1"
                     /translation="MNAAVSLYKHLDEMVPWNDRLQVLEVVGVFDEAPDVKTFTFRSD
                     NQTWFRYKPGQFVTLELPVEGGPVMRTYTLSSSPSRPFSIAVTVKAQAGSIGTRWMFD
                     HLKPGERIKAYGPTGEFSLHNHPSAKYLFISAGSGVTPMMSMLRWLNDFTPQTDVSFV
                     NCARRPEEIIFRKELELLGTRMPGLSLGFLIEERSSRESWFGHMGRIDAVRLPMLSPD
                     FREREVFCCGPEPFMRAVSQMLESSGFDMAHYHQESFAAPLVEPVATPFADSLLAVPC
                     EPAQAALPIRFSLSDIDAECVSGQTVLQTARASGVRIPAACEFGLCGTCKVRKISGEV
                     EMSHNGGILDHEIADGFILSCCSKPLSQLEIEA"
     gene            2100..2369
                     /locus_tag="VGM46_02610"
     CDS             2100..2369
                     /locus_tag="VGM46_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191514.1"
                     /translation="MNDENDVVAAARQIAPATMAKVSPLPPPEEVFIDWLMALPHHAC
                     IEAAARREIARIDRRNLLHADVQRLRVMLLAVAGAIQWRRPLASL"
     gene            complement(2388..3011)
                     /locus_tag="VGM46_02615"
     CDS             complement(2388..3011)
                     /locus_tag="VGM46_02615"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY4191515.1"
                     /translation="MQRVWGRASSVNVMKIVWLLELLRQPYERIDAGRSFGVTDSADY
                     RTMNPNGKIPTLQQDDGFVLWESNPILRYLASTCPGGETYWPADPRRRANVDRWMDWQ
                     QTTIDAPMRVLLRGLVRTPPAERNMAEIEAAVADAGLAWSQLNDELSRHAFVAGENFT
                     LADIVLAVHVHRWFAFDIERPDLPALRAWYDGLLTSPIYRQHGALPL"
     gene            complement(3070..4059)
                     /locus_tag="VGM46_02620"
     CDS             complement(3070..4059)
                     /locus_tag="VGM46_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY4191516.1"
                     /translation="MTSQTSSSHAATAAQMPPLETDHKSLMEGFAYRRIKTRGAEINV
                     AVAGSGPPLLLLHGNPLTHVGWHQVAPTLAKSFTVVAIDLRGYGDSSKPDGGPDHAAY
                     SFRAMGEDGFDVMSELGFEKFALAGHDRGARCGFRMALDQPDRITHFCALDIIPTHHL
                     LTNVTLGWGLESYHWFFMAQKAPFPEKLICADLDYYIQYKLNKKGVGLQIFTPEAFKE
                     YARCTTPEQIHAVCEDYRATVTLDLALDTADFGKKKIQCPVLVLWGSNSHVGRHLKPI
                     EAWTPWAPDLRGWAIPTGHYPAEHRPDLVYESFWNFFNGIEPKPYCADNDSAA"
     gene            4218..5270
                     /locus_tag="VGM46_02625"
     CDS             4218..5270
                     /locus_tag="VGM46_02625"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEY4191517.1"
                     /translation="MAKPENSTGGNLSSLPRMALLLGDMTGIGPELVARVVADKRLAD
                     VARIVVLGDRRVLEMGARDAGVSLSIKTVKDADAVDWDAPEVPLIDLGNIDPATLPRA
                     EVSPHSGKLTGDTLAEAVKLAVKGDFDAITFAPLNKAALHAGGWKFPDEHKMFAHLLE
                     HKGYFSEMNVLDGQWMSRVTSHVSLRTALDQITHESIIEAIQLVDRTMRRAGIDKPRI
                     AVAALNPHAGENGLFGREEIDLIAPAVKAAAATGIDCKGPFPSDTVYIKAFAGEYDSV
                     VAMYHDQGQIATKLRGFNRGVTVTAGLDTVFTTPAHGTAFDIVGKGVASTGALETAVR
                     LAAQLASRKAAASAAA"
     gene            5304..6377
                     /locus_tag="VGM46_02630"
     CDS             5304..6377
                     /locus_tag="VGM46_02630"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HEY4191518.1"
                     /translation="MPQSNSFRIAVIPGDGIGKEVMPEGVRVLEKVAARFGIGLSLDW
                     FDFASYDYYAKHGKMMPDDWKEKIGSHDAIFFGAVGWPAGIPDHVSLWGSLLQFRREF
                     DQYVNLRPVRLMPGVPSPLVGRKPGDIDFWVVRENTEGEYSSIGGRMFPGTEREIVMQ
                     ETVMTRIGVDRVLKFAFELANKSKRKHLTSATKSNGISISMPYWDERVEEMAKAYPDV
                     RWDKYHIDILTAQFVLHPDWFDVVVASNLFGDILSDLGPACTGTIGIAPSANINPDRK
                     FPSLFEPVHGSAPDIAGKGIANPIGQIWSAAMMLEHLGQPEAAAAIVSAIETVLAQPS
                     LRTGDLGGRASTVECGKAIADAL"
     gene            complement(6461..7801)
                     /locus_tag="VGM46_02635"
     CDS             complement(6461..7801)
                     /locus_tag="VGM46_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY4191519.1"
                     /translation="MDVTAILARYVVSAQAASVPANVRQEAVRSFLNWAGCAVGGSQH
                     ETVDIALAALSPFAGQGQAAVLGRTERLDGLNAALLNGIASHVFDFDDTHLKTIIHPA
                     GPIASAILSLAQIQPVSGRDFLHAFILGVEVECRIGNAVYPEHYDIGWHITGTAGVFG
                     AAAAAGKLLGLTEQQMIWALGIAGTQSSGFREMFGTMCKSFHPGRAAQNGLLSAVLAK
                     RDFTSSNRVLEAPRGFAHVMSTQRNFDEITKGLGDSFEIALNTYKPFACGIVIHPIID
                     GCAQLRNEHGLTAAQIERIDLEVDPLVLELTGKKTPQTGLEGKFSVYHSAAVAIIDGK
                     AGEEEYSDARVRNAEVMALRDKVNAVAADNIAEDECRIAVTLKDGKVLRKHVEHAIGS
                     LARPMTNADLDAKFSGLADPVLGAGAATRLRDMCWDIEKLGDVAAIATAATPAH"
     gene            complement(7911..8657)
                     /locus_tag="VGM46_02640"
     CDS             complement(7911..8657)
                     /locus_tag="VGM46_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007753509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY4191520.1"
                     /translation="MSATRELDGRVALVTGAVRNIGRIMALEFANAGAAVIVNARSSK
                     AEAEAVADEIVAAGGRAKVILADVSDPVGAGKLIDEAAAHFGRLDILVNSAAVRRETS
                     FENLGYAEWREVLSTILDGAYLTTHAALAHLRKSDQAAIVNIGGLSAHIGAARRAHVI
                     AAKAGLSGLTRALAHDLAEDGITVNCVSPGMIDTVRTGGEPAHHAKLPPVGRLGAPQE
                     VASFVRYLCGPAARYITGQTIHLNGGAFMA"
     gene            complement(8695..>9289)
                     /locus_tag="VGM46_02645"
     CDS             complement(8695..>9289)
                     /locus_tag="VGM46_02645"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEY4191521.1"
                     /translation="RFSVSRGPVREVCRALAEKGLLELIPNRGVFMRRIDEAEAAELY
                     DLRAGLFAAATRLLAKTATAGQIAYLEGLLGSMETAVSTHSLDDYYPLNLEFHDSILR
                     FTGNSRLHKAYSEIIKELHMYRERALLQGGGLAVSNIEHRRIVTALKTGDANGAMEMA
                     FLHVESGKNRMVLPHDKATVPDADPAPERLVGSGQPS"
BASE COUNT         1678 a         2828 c         3070 g         1713 t
ORIGIN      
        1 cagccgggtc tgtccgtccg agaagggtac ggcggccaag ctggtctgtc cctatcacca
       61 gtggacctac gagttggacg ggcggctatt gtttgcgcgc cagatggcgg aaggcttcga
      121 caaggccgat ttcgggctga aacccatcgc ctgcgagagc gtcggcggct atctcttcat
      181 ttgcctcgcc gacaggccgg ccgatttcgc gctggtgcgc gccatgatcg aaccctacct
      241 tgcgccgcat cggctgaccg aggcgaaggt cgcgttcgaa agcaccatta tcgaaaaagg
      301 caactggaag ctggtctggg aaaacaaccg cgagtgctac cattgcgcgg ccaaccaccc
      361 ggaattgtgc aagacctttc cggaagcgcc gaccgtcacc ggcgtgcagg gcgccaacag
      421 cgatccggaa atgctggcgc attggaccaa gtgcgaggcg gcgggcctgc ccagcgcttt
      481 ccgcatcgat cccgatggcc agtatcgggc gacgcgcgcg ccgcttttgc gcgatgcggt
      541 cagctacacc atgaccggca agcgtgcggt gaagaaaaac ctttccgaca ccgtgacgac
      601 tgagggcatc ggctcgctgc tgctctacca ctatccgacg acctggaacc atattctcgg
      661 tgaccatgcc gtgaccttcc gcgtgctgcc gatcagcgcc accgaaacgg cggtcaccac
      721 caaatggctg gtgcacaagg acgcggtcga gggtgtggac tacgatcttg ccgaactcac
      781 ccatgtgtgg acggagacca acgaccagga ccgccgcatc gtcgaggaaa acgccttcgg
      841 caccctgtcg ccggcctatg agccaggacc ttattcggaa atgcacgagg gcggggtcat
      901 ccagttcgtc aactggtacg ccgatttcat cgggcctcgc ctggcgcagg gtggaaagcc
      961 cgcgctgaag aacgtcgcgt agagggcgcg gcgatgaacg cggcagtcag cctctacaag
     1021 catctcgacg agatggtgcc ctggaatgac cggcttcagg ttctggaagt ggtgggcgtc
     1081 ttcgacgagg ctccggatgt gaagacgttc accttccgct ccgacaacca gacgtggttc
     1141 cgctacaagc caggccagtt cgtgacgctg gaactgcctg tcgaaggcgg ccccgtcatg
     1201 cgcacctata cgctgtcgtc ctcgccgtcg cggccattct ccatcgcggt caccgtcaag
     1261 gcgcaggcag gcagcatcgg cacgcgctgg atgttcgatc acctgaagcc tggcgagcgg
     1321 ataaaggctt acggtccgac cggcgagttc tcgctgcaca accatccgtc agccaaatat
     1381 ctgttcattt cggcagggtc cggcgtcacc ccgatgatgt cgatgctgcg ctggctgaac
     1441 gacttcacgc cgcagacgga cgtgtccttc gtcaactgcg cgcgccggcc cgaagagatc
     1501 atcttccgca aggagcttga gctgcttggc acccgcatgc ccggcctttc cctcggcttc
     1561 ctcatcgagg agcggtcgag ccgtgaaagc tggttcggcc atatgggccg cattgacgcg
     1621 gtgcggctgc cgatgctgtc gccggatttc cgcgagcgcg aagtgttctg ctgcgggccg
     1681 gagccgttca tgcgcgcggt gagccagatg ctggaaagct cgggcttcga catggcgcac
     1741 taccatcagg agagctttgc cgcgcccctg gtcgagccgg tcgcgacgcc atttgccgac
     1801 agtttgctgg cggtgccgtg cgaacccgcg caggcggcgc tgccgatccg cttctcgctc
     1861 tcggacatcg atgccgaatg cgtttccggc cagaccgtgt tgcaaacggc gcgcgcttcg
     1921 ggcgtgcgca tcccggctgc gtgcgaattc ggcctgtgcg gcacctgcaa ggtcaggaaa
     1981 atctcgggcg aggtcgagat gagtcataat ggcggcatcc tcgaccacga gatcgccgat
     2041 gggttcattc tgagctgctg ttccaaaccg ctatcgcaac tcgaaatcga ggcatgacga
     2101 tgaacgatga gaatgacgta gtcgctgccg cccgccagat tgcaccggca acgatggcga
     2161 aagtctcgcc gctgccgccg cccgaggaag tgttcatcga ctggctgatg gcgctgccgc
     2221 accatgcctg catcgaggct gccgcgcggc gcgaaattgc gcgaatcgac cggcgcaacc
     2281 tgttgcatgc agatgttcaa aggctgcgcg taatgctgct tgcggtcgcg ggcgccatcc
     2341 agtggcggcg gccattggcc agcctctgaa gcccggcgga cgccgggcta gaggggcagg
     2401 gcgccgtgct ggcggtagat gggcgaggtc aaaagcccgt cataccaggc gcgcagcgcc
     2461 ggaaggtccg gcctttcgat atcgaaggcg aaccagcgat gaacatggac ggcaaggacg
     2521 atgtcggcca gcgtgaaatt ttcgcctgcc acaaacgcat ggcgcgacag ttcatcgttc
     2581 agctgcgacc aggcgaggcc ggcgtcggcc acagctgctt cgatttcggc catgttgcgc
     2641 tcggccggcg gcgtgcggac aaggcccctg agcagaacac gcatcggcgc gtcgatggtg
     2701 gtctgctgcc aatccatcca gcggtcgaca ttggcgcgcc ggcgcgggtc ggcaggccag
     2761 tatgtttcgc cgccggggca ggtagaggca aggtagcgca ggatagggtt gctttcccac
     2821 agcacgaagc cgtcatcctg ttgcagcgtc ggtatcttgc cattggggtt catggtgcgg
     2881 tagtccgcac tatcggtaac gccgaaggac ctgccggcat cgatgcgctc gtaaggttgg
     2941 cggagcaatt ccagcagcca cacgatcttc atgacgttga cggaactggc acggccccat
     3001 acgcgctgca tgctttatct ccgaaagccg tggcggcgac gggttgtccc tgccgccgcc
     3061 ttcgcgccct tatgctgcgc tgtcgttgtc ggcgcaatag ggctttggct cgatgccgtt
     3121 gaagaaattc cagaagcttt catagaccag atcggggcgg tgttcggcgg ggtaatggcc
     3181 ggtcgggatc gcccagcccc tgaggtcggg cgcccatggc gtccacgcct cgatcggctt
     3241 caagtggcgg ccgacatggc tgttcgaacc ccacagcacc agaacggggc actggatctt
     3301 cttcttgcca aaatcggcgg tgtcgagcgc cagatcgagc gttacggtgg cgcggtaatc
     3361 ctcgcagacg gcgtgaatct gctcgggcgt ggtgcagcgc gcatattcct tgaacgcctc
     3421 gggcgtgaat atttgcaggc cgacgccctt cttgttgagc ttgtactgga tgtaataatc
     3481 tagatcggcg cagatcagct tttcagggaa gggcgctttc tgcgccatga agaaccagtg
     3541 gtagctctcc agcccccagc ccagcgtgac gttggtcagc aaatggtggg tggggatgat
     3601 atccagcgcg cagaaatggg tgatcctgtc cggctggtcg agcgccatgc gaaagccgca
     3661 gcgcgcgccg cgatcgtggc cggcaagggc gaacttctca aagccaagct cgctcatcac
     3721 gtcgaagccg tcttcgccca tggcgcggaa gctgtaggcg gcatggtcgg ggccgccatc
     3781 gggcttggaa ctgtcgccat agccgcgcaa atcgatggcc accacggtga acgatttcgc
     3841 cagggtaggc gcaacctgat gccagccgac atgggtgagc gggttgccgt gcagcaggag
     3901 caggggcggg ccgctgccgg cgacggcgac gttgatttcg gcgccccgcg tcttgatgcg
     3961 gcggtaggcg aagccttcca tcaggctttt atggtcggtt tccagcggcg gcatttgcgc
     4021 ggccgtggct gcgtgcgacg aactggtctg gctggtcatg atgcgtctcc aaaaagctgg
     4081 cgcaacctcg cctcaaaatt gaatactgtc aacaattttc agttgacttt tttatcccat
     4141 ggcgcgacac ccacggcacc gaacaggaaa tgcggacggt ggcgctgccg cccggcacag
     4201 aacaaaggtg acagttcatg gccaaaccgg aaaactccac gggcggcaat ctctcgtcct
     4261 tgccgcgcat ggcgctgctc ctgggcgaca tgaccggcat cgggccggag cttgtggcgc
     4321 gtgtcgtggc ggataaaagg ctggccgatg tggcgcgcat cgtcgttctg ggtgaccggc
     4381 gcgtgctgga gatgggcgcg cgcgacgccg gcgtatcgct ttccatcaag accgtgaagg
     4441 atgccgacgc cgtcgattgg gacgcgcccg aggtgccgtt gatcgatctc ggcaatatcg
     4501 acccggccac cttgccgcga gccgaggttt cgccccattc cggcaagctg accggcgaca
     4561 cgctggcgga agcggtgaag ctcgcggtca aaggcgattt cgacgccatc accttcgctc
     4621 ccctgaacaa ggcggcgctg cacgccggcg gctggaaatt cccagacgag cacaagatgt
     4681 ttgcgcatct gcttgaacat aaaggttatt tttccgagat gaacgtgctc gacggccaat
     4741 ggatgtcgcg cgtcacttcg catgtgtcgt tgcgcaccgc gcttgaccag atcacgcatg
     4801 agagcatcat cgaggcgatc cagctggtcg accgtaccat gcggcgtgcc ggcatcgaca
     4861 agccgcgcat cgcggtggcc gcgctcaacc cgcatgccgg cgaaaacggc ctattcgggc
     4921 gcgaggagat cgacctgatc gccccggcgg tgaaggcggc ggcggccacc ggcatcgact
     4981 gcaaggggcc gtttccttcc gatacggtct atatcaaggc gttcgcgggc gaatatgaca
     5041 gcgtcgtggc gatgtatcac gaccagggcc agatcgcgac caagctgcgc ggcttcaacc
     5101 gtggcgtgac ggtcaccgcc gggctcgata ccgtcttcac cacgccggcg cacggcaccg
     5161 ctttcgacat cgtcggcaag ggcgtcgctt cgaccggcgc gctggaaacc gccgttcgtc
     5221 tggcggccca gcttgccagc cgcaaggcgg cagccagcgc cgcagcgtga gcgcaatcgc
     5281 ttcgagccaa ggagaaccac cgcatgccgc aatccaacag ttttcgcatt gccgtcattc
     5341 cgggcgacgg catcggcaag gaagtgatgc cggaaggcgt gcgcgtgctg gaaaaagtgg
     5401 cggcgcgctt cggcatcggg ctgtcgctgg actggttcga cttcgcctcc tacgactact
     5461 acgccaagca cggcaagatg atgccggacg actggaagga aaagatcggc tcgcatgacg
     5521 ccatcttctt cggcgccgtc ggctggccgg caggcattcc cgaccacgta tcgctgtggg
     5581 gatcgctttt gcagttccgg cgcgagttcg accagtatgt gaacctcagg ccggtgcggc
     5641 tgatgccagg cgtgccaagc ccgctcgtcg gcaggaagcc cggcgacatc gacttctggg
     5701 tcgtgcgcga aaacaccgag ggcgaatatt cctccatcgg cggcaggatg ttccccggca
     5761 ccgagcgcga gatagtgatg caggaaacgg ttatgacgcg catcggcgtc gaccgggtgc
     5821 tgaaattcgc cttcgagctg gccaataaaa gcaagcgcaa gcacctgacc tcggccacca
     5881 agtcgaacgg catttccatc agcatgccct attgggacga gcgggtggaa gaaatggcca
     5941 aggcctatcc cgatgtgcgc tgggacaagt accacatcga tatattgacg gcgcagttcg
     6001 tgctgcatcc cgactggttc gacgtggtgg tggcgtccaa cctgttcggc gatattctgt
     6061 ccgaccttgg gccggcctgc acgggtacga tcggcattgc gccgagcgcc aacatcaacc
     6121 cggatcgcaa gtttccctcg ctgttcgagc cggtgcatgg ctcggcgccc gatatcgccg
     6181 gcaagggcat cgccaatccg atcggccaga tctggtcggc agcgatgatg ctggaacatc
     6241 ttggccagcc ggaagccgcg gcggcaatcg tttcggccat cgaaaccgtg ctcgcccagc
     6301 catcgctgcg caccggcgac cttggcgggc gcgccagcac ggtcgaatgc ggcaaggcca
     6361 tcgccgacgc gctttgagaa ttgtccctgc caaaacgccg ccgccttatc aaaaggcggc
     6421 ggcgtttttc atggatggcc gatggtatca gcgcgtgttt tcagtgcgcc ggcgtggcgg
     6481 cggtggcgat ggcggcaacg tcgcccagct tttcgatatc ccagcacata tcgcgcagcc
     6541 gcgttgcggc cccagcgccg agaaccgggt cggcaagtcc gctgaacttg gcgtccaggt
     6601 cggcattggt catcgggcgg gccaggctgc cgattgcatg ttcgacatgc ttgcgcagca
     6661 ccttgccatc cttcagcgtg acggcgatgc ggcactcatc ctcggcgatg ttgtcggcgg
     6721 cgacggcgtt gaccttgtcg cgcagcgcca tcacttcggc attgcgaacg cgcgcgtcgc
     6781 tatattcttc ctcgccggcc ttgccgtcga tgatggcgac cgccgcggaa tggtagacgc
     6841 tgaacttgcc ttcgaggccg gtttgcgggg tcttcttgcc ggttagttcc agcaccagcg
     6901 ggtcgacttc gaggtcgatg cgctcgatct gcgcggcggt caggccgtgt tcgttgcgca
     6961 gctgcgcgca gccatcgatg atcggatgga tgacgatgcc gcaggcgaac ggcttgtagg
     7021 tgttgagcgc gatctcgaag ctgtcgccaa ggcccttggt gatctcgtcg aaattacgct
     7081 gcgtggacat gacatgcgcg aagccacgcg gcgcttccag cacgcggttg gacgaggtga
     7141 agtcgcgctt ggccagtacg gccgaaagca ggccgttctg ggcggcacgt ccaggatgga
     7201 agctcttgca catggtgccg aacatttcgc ggaaaccgga tgattgcgtg ccggcgatgc
     7261 cgagcgccca gatcatctgc tgttcggtaa ggccgagcag cttgccggct gcggctgctg
     7321 ctccgaacac gccggccgtt ccggtgatgt gccagccgat atcgtaatgt tcggggtaga
     7381 ccgcattgcc gatgcggcat tccacctcga cgccgaggat gaaggcatgg aggaaatcgc
     7441 ggccgctgac cggctggatc tgcgcgaggc tgaggatagc ggaggcaata ggcccggcgg
     7501 gatggatgat tgtcttcaga tgggtgtcgt cgaaatcgaa aacatggctg gcaatgccat
     7561 tgagcagcgc cgcgttgagc ccgtccagtc tttcggtgcg gccgagcacg gcggcctggc
     7621 cctgtccggc aaaaggcgaa agcgcggcca gagcaatgtc gacggtttca tgctgcgagc
     7681 cgccgacggc gcagcccgcc cagttcagaa acgagcgaac cgcttcttgc cggacattgg
     7741 ccggcacgct cgccgcctgc gcggaaacga cataacgggc cagtatggcg gtgacgtcca
     7801 tgagggttct ccttgaatag ggtgccggaa atctggcgtt gggaatctgg cgccggcggc
     7861 ggttgggtca accgccggat ggcattgaat atgagggttg acgtgagagg tcacgccatg
     7921 aaggcgccgc cgttgaggtg gatggtctgg ccggtgatat agcgcgccgc cggcccgcac
     7981 aggtagcgga cgaacgacgc cacttcctgc ggagcgccaa ggcggccgac cggcggcagt
     8041 ttcgcgtggt gggcaggctc gcccccggtg cgcacggtat cgatcatgcc gggcgatacg
     8101 cagttgaccg tgatgccgtc ttcggcgagg tcgtgggcaa gcgcgcgggt caaacccgaa
     8161 aggcccgcct tggcggcaat gacgtgagcg cggcgggcgg cgcctatatg cgccgatagt
     8221 ccaccgatgt tgacgatggc ggcctggtcg gacttgcgca gatgcgcgag cgccgcatgc
     8281 gtggtgagat aggcgccatc gaggatggtc gacagcactt cgcgccattc ggcgtaaccg
     8341 agattttcga aggaggtctc gcgccggacg gcggcgctgt tgacgagaat gtcgaggcgg
     8401 ccgaaatggg cggcggcctc gtcgatcagc ttgcctgcgc cgacggggtc ggagacatcg
     8461 gccaagatga cctttgcccg gccgccggca gccacgatct cgtcggcgac ggcttcggcc
     8521 tcggcctttg aacttctggc attgacgatc accgcagcgc ctgcattggc gaattccagc
     8581 gccatgatgc ggccgatatt gcggacggcg cccgtcacca gggcgacgcg accgtcgagt
     8641 tcgcgggtgg cgctcatttt gttctcccga agatttgccg acatcccatg gccatcagga
     8701 gggttgcccc gacccgacga gcctttcagg tgcgggatcg gcgtccggca ccgtggcttt
     8761 gtcatgcggc aggaccatgc ggttcttgcc gctttctaca tgcaggaacg ccatttccat
     8821 cgcgccgttg gcatcgccgg ttttcagggc ggtgacgatg cggcgatgct cgatattgga
     8881 tacggcaagg ccgccaccct ggagcagggc gcgctcccgg tacatgtgca gttccttgat
     8941 gatctcggaa taggccttat gcaggcggct attgccggtg aagcgcagga tggaatcgtg
     9001 gaattccaga ttgagcgggt aatagtcgtc aagggaatgg gtggagaccg ccgtttccat
     9061 gctgcctagc aaaccttcga gataggcgat ctgcccggcg gttgcggttt tcgccagcag
     9121 ccgggtggcc gcggcgaaca ggccggcacg cagatcgtag agttcggcgg cctcggcctc
     9181 gtctatgcgg cgcatgaaca cgcctcgatt ggggatcagt tcaagcagcc ccttttcggc
     9241 aagcgcgcgg caaacctcgc gcaccggccc gcggctgacg gaaaagcgg
//
LOCUS       DASYCS010000075         3328 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3205885,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000075 DASYCS010000000
VERSION     DASYCS010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3205885"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            286..2166
                     /locus_tag="VGM46_02650"
     CDS             286..2166
                     /locus_tag="VGM46_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008877701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4191522.1"
                     /translation="MFSWFEKRLDPFPATEPVEPPKTLIAFCVHYTRGTWPYIAATAS
                     LITAIALAEVWMFGFLGHIVDWLSAQNRETFLQTEGWKLAAMAFVVVVVLPAMALLQS
                     LVHDQTLMGNYPMRIRWDVHRYLLKQSMSFYQDEFAGRIATKLMQTALSVRECVVKVI
                     DVLNYVIVYFLGMLFIVGSSDWRLAMPLLGWLACYALLLRHFIPKLGKVSAEQADARS
                     LMTGRIVDSYANIQTVKLFSHARREATYARESMAIFMDTVYRSMRLVTSLYGMLYLIN
                     SLLLFAVSALSIWLWLGDSVSIGAVAVVIGLVLRLWGMSQWIMWEMSALFENVGTVQD
                     GIGSISLPRLVEDRPEAKDIVVERGDIRFEEIGFHYGKGKGVIDKLSLTIRPGEKVGI
                     VGRSGAGKSTLVNLLLRFYDLESGRIVIDGQDIAAARQDSLRANIGMVTQDTSLLHRS
                     VRDNILYGRPDAGEAMMLEAARRAEALDFIATLADAKGRTGFDAHVGERGVKLSGGQR
                     QRIAIARVMLKDAPILILDEATSALDSEVEAAIQENLYRLMKGKTVIAIAHRLSTIAA
                     MDRLVVMDKGRIIEEGSHDELVATGGLYAQLWQRQSGGFLPDDALSEGAAYAAAKDEA
                     AE"
     gene            2163..>3328
                     /locus_tag="VGM46_02655"
     CDS             2163..>3328
                     /locus_tag="VGM46_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4191523.1"
                     /translation="MMNAVYRWFESWVYPFREPASLKPPASVGGFLWHYVGQAKFAFF
                     AMLVIGGIAPLVEAGLFYFVGRVVDILDQLSGPRSWQALWNAAGPELLFMIGVVLVVR
                     TIVVGLSALVNEQTITPGFYNLVRWQAHRHVSRQSYAFFQNDFAGRIATKVWQAGQAT
                     GDLMESFIEVVWFMLVYTVTTLALVAGLDWRLAALVVVWIVAFGLLAKLYLPAIRRHA
                     EATAEAGSMINGRIVDSYSNVQTLKLFSADGDDRYIRSGFDVYLDALRPFTRRLTGVR
                     MALTALSGIMITAIACFSVYLWVEGLITVGAVAFTLSLVLRLNMLLGRLMMQFNGILR
                     NLGVLENSKALISQPLGLTDAPDATELVVAKGRIEVKNVEFHYGKGFGVLNGIDL"
BASE COUNT          569 a          973 c         1064 g          722 t
ORIGIN      
        1 ggcggtccat tgtcaactcg aaccgatggg ggaaccgatg atgtccagct acaccaatcc
       61 accccttgct tggctgatgt gtcagccggc ggttccggtc cgacgctttt tctgaatttt
      121 caagcacgct cgcactggtc ccgccgaaac gccgttgcgg tattcgaagg acatttgcga
      181 tgtttcccgc ctgccgaaaa gattgaatcc ggtcgaatag ctgccctttc gctatttcct
      241 gcggttcgtt tcaggcgccc gatttcgatg gaagagtaag agacgatgtt tagctggttt
      301 gaaaaaaggc ttgacccttt ccccgccacc gaaccggtgg agccgccgaa gacgctgatc
      361 gccttttgcg tgcattatac gcgcggtaca tggccctaca tcgccgccac cgcctcgctg
      421 atcacggcaa tcgccctggc ggaagtctgg atgttcgggt ttctcggcca catcgtcgac
      481 tggctgtcgg cgcagaaccg cgagaccttc ctgcaaacgg aaggctggaa gctggccgcc
      541 atggcattcg tggtcgtcgt ggtgctgccg gcgatggcgc tgttgcagtc gctggtccac
      601 gaccagacgt tgatgggcaa ctatccgatg cgcatccgtt gggacgtgca ccgctacctg
      661 ctcaagcagt cgatgagctt ctaccaggac gagtttgccg gacgcatcgc caccaagctg
      721 atgcagacgg cgctgtcggt gcgcgaatgc gtcgtcaagg tgatcgacgt cctgaattat
      781 gtgatcgtct atttcctcgg catgctgttc atcgtcgggt cttccgactg gcggctggcg
      841 atgccgctcc tggggtggct ggcctgctat gccctgctgc tgcgccattt catccccaaa
      901 ttgggcaaag tgtcggccga gcaagccgat gcgcgctcgc tgatgacggg gcgcatcgtc
      961 gattcctacg ccaatatcca gacggtaaag ctgttttcgc atgccaggcg cgaggcgacc
     1021 tatgcgcgcg aaagcatggc gatcttcatg gacaccgtct atcggtcgat gcggctcgtc
     1081 accagcctct acggcatgct gtatcttatc aattcgctgc tgctgtttgc cgtctcggcg
     1141 ctgtcgatct ggctgtggtt gggcgattcg gtgtcgatcg gcgcggttgc cgtcgtcatc
     1201 ggcctggtgt tgcggctgtg gggcatgtcg caatggatca tgtgggaaat gtcggcgctg
     1261 ttcgagaatg tcggaacggt gcaggacggc atcggctcga tctcgctgcc gcgccttgtc
     1321 gaggacaggc cggaggcgaa ggatattgtc gttgagcgcg gcgacattcg tttcgaggaa
     1381 atcggcttcc attacggcaa gggcaagggc gtcatcgaca agctgtcgct gacgatccgg
     1441 cccggcgaaa aggtgggcat tgtcgggcgc tccggcgccg gcaagtcgac gctggtcaat
     1501 ttgctgttgc gtttctacga tctggaaagc ggccgcatcg tcatcgacgg gcaggacatt
     1561 gccgccgccc ggcaggattc gctgcgcgcc aatatcggca tggtcacgca ggatacatcg
     1621 ctgctgcacc gctcggtgcg cgataatatt ctctacggcc gtcccgatgc tggcgaggcg
     1681 atgatgctgg aagcggcaag gcgggccgaa gcgctggact tcatcgcaac gcttgccgac
     1741 gccaagggcc gcaccggttt tgacgcgcat gtcggcgagc gcggggtcaa attgtcggga
     1801 ggtcagcggc aacgcatcgc cattgcccgc gttatgctga aagatgcgcc gatccttatt
     1861 ctggacgagg cgacctcggc gctcgattcg gaagtcgagg cggccattca ggagaacctc
     1921 tacaggctga tgaaaggcaa aaccgtcatc gccatcgcgc accggctttc gacgatcgcg
     1981 gcgatggacc ggctggtcgt catggacaag ggccggatca tcgaggaagg ttcgcacgac
     2041 gaactggttg cgacaggcgg gctatacgcg cagctctggc aacgccagtc gggcgggttc
     2101 ctgcctgacg acgccctatc ggaaggtgcg gcatatgccg ccgcaaagga cgaagccgcc
     2161 gaatgatgaa tgccgtttat cgctggttcg aatcctgggt ctatccgttc agggagcctg
     2221 cgagcctcaa accaccggcc agtgtcggcg gttttctctg gcattatgtc ggccaggcca
     2281 aattcgcctt ctttgccatg cttgtcatcg gtggcatcgc gccgctggtg gaagcggggc
     2341 tgttttattt cgtcggccgg gtggtcgata ttctcgacca gctctccggc ccgcgcagct
     2401 ggcaggcgct gtggaatgcc gccggccccg aactgctgtt catgatcggc gtggtgctgg
     2461 tggtgcgaac catcgtcgtc ggcctgtcgg cgctggtgaa cgagcagacg atcacgcccg
     2521 gcttttataa ccttgtgcgc tggcaggcgc atcgccacgt ttcgcgccag tcctatgctt
     2581 tcttccagaa cgatttcgcc ggccgcatcg ccaccaaggt gtggcaggcg gggcaggcga
     2641 cgggcgacct gatggaaagc ttcatcgagg tcgtctggtt catgctggtc tatacggtga
     2701 cgacgctggc gctggtcgcc ggactggatt ggcggctggc ggcgctggtg gtggtgtgga
     2761 tcgtcgcctt cggcctgctg gccaagctct atctgccggc catccgccgc catgccgagg
     2821 caaccgccga ggcggggtcg atgataaatg gccgcatcgt cgattcctat tccaacgtgc
     2881 agacactgaa actgttttca gccgatggcg acgaccgcta tatccgcagc ggcttcgatg
     2941 tctatctcga cgcgctgcgc ccctttacgc ggcggctgac cggcgtgcgg atggcgctga
     3001 cggcgctgtc cggcatcatg atcacggcca tcgcttgctt ctcggtctat ttgtgggtcg
     3061 aaggcctgat tactgtcgga gctgtcgcct ttacgctcag cctcgtgctg cggctcaaca
     3121 tgctgcttgg gcggctgatg atgcagttca acggcatatt gcgaaatctc ggcgtgctgg
     3181 aaaactccaa ggcgctgatc tcgcagccgc tcggcctgac cgacgcgccg gacgccacgg
     3241 aactggtggt cgccaagggc cgcatcgagg tgaaaaacgt cgagttccat tacggcaagg
     3301 gatttggcgt gctgaacggc atcgacct
//
LOCUS       DASYCS010000076        19096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4944114,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000076 DASYCS010000000
VERSION     DASYCS010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 19096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19096
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4944114"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1157)
                     /locus_tag="VGM46_02660"
     CDS             complement(<1..1157)
                     /locus_tag="VGM46_02660"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY4191524.1"
                     /translation="MSAHKDTVARAPIEYPTYAERMAEIFAGLKLERISPKAKEIAVN
                     DLIDMTGLCVAARKADYIASIVEGWDGEGSCTAIGHRRGFDAAGAALINGTATHGEDF
                     DDTLEGAPIRVGAMVLPAVLAACERHGRSGNDALLGMIAGLEAVCRLNRVVPGAMHTA
                     GFHPVGIIGTFGATVGAGVALGLDARQLTLALGIAGSFASGIGEFLTEGAWTKRLHPG
                     WAAHSGYRAATLGRAGFVGPRTVFEGNRNVFRVFSHGAQENYTPLLEGLGSVWQMEDI
                     AFKPYACGTMIHPFIDCTIRLAAQGVQASDIVSIVCETGEGLVNRLWEPLAAKHLPPS
                     GYAAKFSMPFCMAVGFFDGDAGLEQFTDEKARDPKILELASKISYVIDPEN"
     gene            complement(1154..1969)
                     /locus_tag="VGM46_02665"
     CDS             complement(1154..1969)
                     /locus_tag="VGM46_02665"
                     /EC_number="4.4.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF014709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HEY4191525.1"
                     /translation="MSQGVEAELAFSFIRTAARPAKPRQSGMTFLADRGMGLNRLADI
                     IETAGDHIDFFKMGIGAWRLLRPDLLRRKIAMLKQNDIGVFLAGDATEAAFMQGVSRR
                     FYEAVKDFGADAVEVSSAQVAMALEDKCRLISMAGDAGLKVVAEVGQKSHGDWTSSQG
                     YVFRQIEDYQKAGAWKVLVQADGISEDVESSRWDIVLAMAARFDLKDLIFQAKDAAAQ
                     EWYVSTLGNKVNLDVDDHQALEIELVRRGIRKRHIFGLVGSLETDDAGREAQS"
     gene            2079..3152
                     /locus_tag="VGM46_02670"
     CDS             2079..3152
                     /locus_tag="VGM46_02670"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191526.1"
                     /translation="MLVLSNEEAEALLHMPECMDALEQTYRQAAEGTAVNGRRSDMIT
                     STTRPDSVYCLKLVGGVVPALKIGALRLNSDILSFPEKNGSRRKVKVPAAAGDRWVGL
                     VMLFSTETGEPLAIFPDGVVQRARVGATSGLAVRHLARPDAKRLAILGSGWQAGAQAM
                     AAAAARPLSEIRCYSPNLDNARQFAGDLEKSLGIAASPAASAKEAVEGADIVLCATNS
                     LAPVLDADWLQEGVHLGSIRDRELPAAAILKASKVFIHHPDNMGSDHLVVASDVTYDE
                     GVKAITSDPTLEPFTRAPTLADLVSGRAQGRTSADDITCFLNYHGLGYQFAAVGAVFY
                     RKALEAGCGHALPTEWFTENVHP"
     gene            3192..3953
                     /locus_tag="VGM46_02675"
     CDS             3192..3953
                     /locus_tag="VGM46_02675"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY4191527.1"
                     /translation="MIEQTALHIDALTGLLVAAICFLAAAVGGLSGFGSGLIMSVAMA
                     PLVGVKAVVPLISVAMIFTNAARIWVYRASFNPRIAVILLATSIPASVLGAIVYVNLD
                     ARLVSIVFGFVLVLSMPVNHFLRRRRLSLTPWTLAPLGLIFGFLSSNIIGAGMIIPPI
                     LIGSGLLGPAVIASDAAIAVGTSVFKVMTFGAMDALTPPLALYGVVMGLCTIPGTWAS
                     AWLMARTSLRLHTALIEACIAVAGINFIWQGFNAA"
     gene            4001..4513
                     /locus_tag="VGM46_02680"
     CDS             4001..4513
                     /locus_tag="VGM46_02680"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEY4191528.1"
                     /translation="MQSNRFLKGDTITAVALIAFAGIFWVAADRLPKSALGGGVGADG
                     LPKFLAVALGLLSLILLVQSLLRKEQKPTEEPGSDEDKPISLYAHMTSLGILVIGGIS
                     VLLLPILGYTATIALLLVAMALYHGQKFTWTVAIYGVLGAVLLYLVFEKVLAVPMPEG
                     IWPGLFGLQS"
     gene            4526..6019
                     /locus_tag="VGM46_02685"
     CDS             4526..6019
                     /locus_tag="VGM46_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007753513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEY4191529.1"
                     /translation="MQNFHYMIDAITSWSALLAVVVGVLWGICGGALPGISASIAMAL
                     LLPFTFSLEPMTAMILLSSVYIGAEYGGSIPAILIRTPGTNSAAATVFDGYEMHKQGK
                     SGEALGLSLVGGTIGSLVGLLVLIGFTEPLSRFALLFTPAAYFAIGLLGISVVASLSS
                     DAPVKGLIAGVLGLGLATIGTDPLSGVARFTFGRAELLGGISVAVVMIGIFAVSELMI
                     QRQSGDHEVDVTGTPPKLVIPSLKTLWDLRVPQIIGWIIGTIEGAVPGAGGSVAAFMS
                     YNEARRWSKEPEKFGKGSPEGVIAPETANNVVASTALMPTLSFGIPGSNSTAVLLGGL
                     LIHGLQPGPLLFSTHPDVVYGLFGAMFMAIAAMFVLGTVILGPVIWLVRRPKPYLQGA
                     IFALLISGIYSIDDSAFDVGLILLIGLVSYVLRVLKFPTLPMVLGVVLGFMIESNYRR
                     SLLISRGDHMIFLRDPACAVLLCLVALMLVTSVWREMRPRKRTPKEA"
     gene            complement(6108..6590)
                     /locus_tag="VGM46_02690"
     CDS             complement(6108..6590)
                     /locus_tag="VGM46_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018235756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191530.1"
                     /translation="MQSFQQILLLIGRLNYTWTNTESLLIHLIAGLARVDKETAIVIF
                     LTLNTTRARIDLVERLAKLPRVPEKCKRDILELLERLSQQSKLRNKYNHCIYSFDDKG
                     DISHTQLMRIFDGKDSIKYGKVEALDAAEAARISDCIEDIADVNKKIWAVVHEHGFPQ
                     "
     gene            7127..8998
                     /locus_tag="VGM46_02695"
     CDS             7127..8998
                     /locus_tag="VGM46_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006728277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191531.1"
                     /translation="MSPRNSHSSSPYILDPSQDQSQDQSQGEAQAQLQAELQGQGQGQ
                     YQSSHSENENGNGNGNLNGNGNLNLNGNASLNGNLNANLNRSINESTNTNSSTNTNSN
                     TNTSDTHVNVDVGVDLTGYEPSNRDVNNIKLDKGAEIDKLLVNHDGQIFYNPGDGATF
                     NDFLNGSLTGEGNDSALSVAQVNNLDDSDHIGRASVSNDGTFEQNGDAQGGDASSGSG
                     ITAGGGGGGGGGHGMASGGDGGNGHSLGGIGIGGSALGAARGGDATGGAGAAGGAGVG
                     LGLGLGAGIGEGGAAGGDGGNANGGDSTGNGGAGLGAGLSGAGGGDSGNADSGDGGNA
                     AGLGLGGAGSGDAGNGDSGNGGDAGGLGIGAGLGDGGNAGDASADGGGALGLGLGLIG
                     VGVGVAGDAGQATGGAADGGAGSGSGSGAGTGGDSGNANGGDSGDATGLGLGSGLGGD
                     SGNANSGDAGDALSLGLGLGHGGDAGDGGLGGMAGLGGDALGLGIGTGFGEGTGAGVG
                     GAGGMGGFAWSGDTIVVPVAGDGTGGASSGGAGGMGGDGGIVTNGAGGAGGDGGTWGT
                     NNGHDSYVNGLSEAHADAVIDTSAFNQSIVMGANMMENLLSQTVVGGDYDNMDHSGS"
     gene            9107..11197
                     /locus_tag="VGM46_02700"
     CDS             9107..11197
                     /locus_tag="VGM46_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion protein"
                     /protein_id="HEY4191532.1"
                     /translation="MHLDKITEAIAHFVGLFQIETEQARLRSDYLEFKALRAQATAPD
                     QHDRAELTFQSPYNFNDPDPGLHYVPAAPTIEPLTVATNVTYAMPQIPVPGEIEAVIY
                     PGFMTPRLLQQDLHTGTRIIELAPPGSVAVHISQINSLSDNDFVNIGTDAVAFHSIGT
                     PNLTLDTLLSEAAKFVPIEAATFASAHDIGDFIADTGASLHAFAAQANGSTTETADGV
                     SIQTNVTVIDAPENNAVYVNGHMQSEAPKLEDVLPAASPLVKDDVATPDDAPGNNHLI
                     NGPGSTATGGVAHGEGAASNASSVEISTGANALVNTATLVNDAIEGNVFAVAGNHFAL
                     NAIIQVNAWSDCDSIGASLNNWDAAGHAAATATFNIASFQHIDTGGDLPGTGDPAQMT
                     FPKAWAVTEIHGDFVSMNWIQQLNFMIDNDNVVSASSSGVMTKLGTGENQGLNDLSIA
                     DLGRYYDLILIGGHYYDANIITQTNVLLDDDIVGAVSGFHTSGQGSVSGSDNLLWNSA
                     AITSIGHDTTGALPTGYSTALDGFAAGNHTLPSSVLADGAFEGLSGLKVLYISGSVYD
                     LQYVSQTNVLGDADHIALAMNSAEANLDASWTITTGSNALLNSAQIIDVDPAGKIYVG
                     GDHYSDELLVQTDIIRTDHLLEGKDADHLVNEAVAFLSDDMMPTQADDASSHLRDAVS
                     VHPAHSDIMQSVVS"
     gene            11217..13412
                     /locus_tag="VGM46_02705"
     CDS             11217..13412
                     /locus_tag="VGM46_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014329915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HEY4191533.1"
                     /translation="MNEHVTSTQQTPPAGDGPEPDHVRGPTPKAASSLKDIEVSFEVL
                     MAEIDQAAAELKRGSRPIFFRPLDTDLPPDRGPAPSAPMEIDQPAVKGGASGSIAAGA
                     LKSIDGDSGPIKASDAPAIQVLKERGKEPASTISSPSAANLPSPTSQVFHRRLGSINL
                     ADNLNAGIHAVRRNLLVVMGFTLATNVLVLAIPVYLFQISDRVLNSRSTDTLVMLTIL
                     IAGAVILNAVLDAIRRFILMRTAVEVAAQLGAPILGAAARASLNSNGREYQTLGDLQQ
                     LRAFLVSGTLLSFLDAPLTPLFMLAVFLVHPHLGYIVVISALLLLVIAFINQRATQAP
                     FHEAGNFQSRANLHLDSMSRNSQIINALAMIPEAVKIWGRDTAGALKAQVVAQDRNVI
                     AASVSKAVRMLAQVAMLGWGAYLSLEGQLTGGMVIAASIIAGRALAPVEGAIEGWHQF
                     INTRSAYQRIAALLRASPLNFERLRLPRPQGRLDVERLLFVPQGTKRVVLNGVTFTLS
                     PGESLAIIGDSGAGKTTLGKMLVGSILPTSGNVRLDLMDLRNWDQRQFGESIGYLPQD
                     VQLFPGTIKANIGRMRDDASDEDIYKAAMLADVHELISTFAQGYETTVAADGSPLSGG
                     QKQRIALARAFFGNPRLVVLDEPNSNLDGTGDAALLRALAHAKQEKITVITITQRPSL
                     LNCVDKIMLLANGTVSMFGVRQDVLKALAPLNTANARNAVGHQPAQATP"
     gene            13409..14746
                     /locus_tag="VGM46_02710"
     CDS             13409..14746
                     /locus_tag="VGM46_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006200198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HEY4191534.1"
                     /translation="MNTSIADVHDIEWYGQVPRSIRTHAFVGLALIVVAFGGFGTWAA
                     TAPLAAAVVTAGSFVATGENKVVQHLEGGIIDDILVSEGDHVTAGEPLIRLDGIAAQA
                     KQRQLFLRQARLEANVARLEAQIDAKSEIAFPTIITNNAGDSDIKAIIDGQLASFRSA
                     RNRIDSETGLLKLNMESLKFRTEGFQRQSASMAEQLDYLKQEYAGKLQLLAQGLIRKP
                     EVNAIQRAIADASGQIGRLDSEMSETNSQIEKFHQQINQTVTELTQKALDELQGSQGD
                     LDGVRQEARQAKNITQRSTINAPVSGTVVRMYYHTSGGVIESGKKILEILPSDVPLII
                     ETQIQRKDIDAVKPGQKATVRLVALNRRTTPVLNGDVYYVSADALSDATIPNQEIYLA
                     RVRLSPAELARIPGFIATPGMPVDVMIETAERTFLDYLTKPIRESMSRAFTER"
     gene            complement(14791..15576)
                     /locus_tag="VGM46_02715"
     CDS             complement(14791..15576)
                     /locus_tag="VGM46_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019170312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY4191535.1"
                     /translation="MRNSHSNFTANLKLACSTRQSISDVCRTIDINRQQFNRYINAGA
                     LPSAHNRLRIAAAFDLEADDFDLPAHIFRQKLKENRPSAMAEGPLAEAFPGDLPALRP
                     HVGFYQTWHISLSWPGRAVCSCSHLRERNGQMLVTSLERIEDVQSGIRQRSRYVGLAA
                     FRRQRIFLTELTRGEQPTFGQTILMPFEIHQRKYLRGLTMGVSWRSGNEPYATRTIWL
                     HRGLSTDIRALIANCGIYELTSASLPSVAASYLAETRPVAVRL"
     gene            15681..17246
                     /locus_tag="VGM46_02720"
     CDS             15681..17246
                     /locus_tag="VGM46_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY4191536.1"
                     /translation="MTDFDNAFSLSRRGFLQGSALLGLSATTGTLVASPARAQTPVKG
                     GTLRMGLGGGASADTLDPALATAAVAFIVAHCWGDTLLESHPETGEPLPSLAESWTPS
                     PDGKEWTFKIRKGVSFHDGKPMTPADVVATLRRHADAGSKSGALGLLSDIKQIEERAG
                     DVVITLAEGNADLPLLLTDYHLQIQPEGGTKNPTAAIGTGPYKLVAFEPGVRAAFTRN
                     TNDWRDDRGHVDSVEITVMNDSTARINALSAGQVDFINTVEPKVVPLLSKAPNVEILQ
                     TSGKGFYSFLMHCDTAPFDNNDLRLALKYAIDREAILKRVVGGFGTIGNDYPVNANYA
                     LAPTDIEQRVYDPDKAAFHFKKSGHDGSLLLLTSEAAFPGAVDAAQVFQQSAAKAGIK
                     LDVQRSPSDGYWSEVWNVKPFCASYWGGRPTQDSRYSTSYVSNAEWNDTRFKRPDFDA
                     MAMQARAEFDQDKRRALYRKMALMVRDEGGLILPVFNNFVNASSKHLKGFVHDIGNDA
                     SNGRIASRVWLES"
     gene            17322..>19096
                     /locus_tag="VGM46_02725"
     CDS             17322..>19096
                     /locus_tag="VGM46_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEY4191537.1"
                     /translation="MPDMNFHIIENEWIVLKDGTRLAARIWMPDSAAAGAPVPAVLEF
                     LPYRKRNGTAPRDESTYPVFAAAGIAGVRVDIRGSGESDGVIDGEYTPRELADAVEVI
                     EWIAAQGWSNGNVGMMGISWGGFNCLQVAALKPPALKAVISIASTVDRYNDDIHYKNG
                     THLSAHFSWAATMSAYQSRSPDPEIVGERWFGMWLERLEQEPFFMEEWLSHQRRDAFW
                     RHGSICENFGEFPVPALVIAGWADGYRNTPLKAVTGMPDKAVALIGPWVHKYPHFAYP
                     KPRADFHGEAIAWWRRWLCGEQNKVEQTPQVRAFILDGPKPAGWRETDPGYWVAKEKW
                     AKPEVATLNPSASGQLEFNPATRASGHALLRSPLDTGTAAGSFFTVKPDAELAGDQRL
                     DDAGSLFFDSEPLTEECVILGQPAFKARLSSDAPLANLAARLVDIHPDGTATRVSFGV
                     LNLAHRAGSAEPQPLEPGKPVEIEMALDACGYRFAPGHRIRLALSSAYWPMILPPPYN
                     AALDLDLATLKLELPLLGAHRRVEVAEPSNPDPLPRYETLSKGSSARSVERDLANGVT
                     RYRISEDGGLNRHPGNGLSTRDAHE"
BASE COUNT         3582 a         6094 c         5867 g         3553 t
ORIGIN      
        1 tcgttttccg ggtcgatgac gtaggaaatc ttggatgcca gctccagtat tttcgggtcc
       61 ctggctttct cgtcggtaaa ctgctccaga ccggcgtcgc cgtcgaagaa accgaccgcc
      121 atgcagaacg gcatgctgaa cttggcggca tagccggaag gcggcaagtg cttggcggcc
      181 aatggctccc atagccggtt gaccaggcct tcgccggttt cacatacgat cgagacgatg
      241 tcgctggcct gcacgccttg tgcagccagc cggatcgtgc agtcgatgaa gggatggatc
      301 atggtgccgc atgcgtaggg cttgaaggcg atgtcctcca tttgccacac gctgcccagc
      361 ccttccagca atggcgtgta gttctcctgc gcgccatggc tgaaaacacg gaaaacattg
      421 cggttgcctt cgaacacggt tcgcgggccg acaaagccgg cacggccaag cgtcgccgcc
      481 ctgtagcccg aatgcgccgc ccagccggga tgcaggcgct tggtccaggc gccctcggtc
      541 aggaattcgc cgatgccgga tgcgaagctg ccggcaatgc cgagcgccag cgtcaactgg
      601 cgcgcatcaa gcccaagcgc cacgccggcg cccacggtcg cgccaaaggt gccgatgatg
      661 ccgaccgggt gaaacccggc ggtatgcatc gcgccgggca cgacgcggtt cagccggcaa
      721 acggcctcca ggccggcgat catccccaaa agggcatcat tgccgctgcg cccatgacgc
      781 tcgcaggccg ccagcaccgc cggcagaacc atcgcgccga cacggatcgg cgcgccttcc
      841 agcgtgtcgt cgaaatcctc gccatgcgtc gcggtgccgt tgatcagggc agcgccggcc
      901 gcgtcgaagc cgcggcgatg gccaatggcg gtgcaggaac cttcgccgtc ccagccttcg
      961 acgatgctgg ctatgtaatc ggctttcctg gccgccacgc aaaggcccgt catgtcgatc
     1021 agatcgttga cggcgatttc cttggccttg ggagaaatcc gctccagctt cagcccggcg
     1081 aagatttcgg ccatgcgctc ggcgtaggtg gggtattcga ttggcgcgcg cgccacagtg
     1141 tccttatggg cgctcatgac tgcgcctccc ggccggcgtc atccgtttcc aggctgccta
     1201 ccagcccgaa gatgtggcgc ttgcgaatgc cgcgccgcac cagttcgatt tccagcgcct
     1261 gatggtcgtc gacatcgagg ttcaccttgt tgccaagcgt gctgacatac cattcctggg
     1321 cggcggcatc cttggcctgg aaaataaggt ccttcaggtc gaagcgcgcc gccatggcca
     1381 gcacgatatc ccagcggctg ctttccacat cctcgctgat gccgtcggcc tgaacgagaa
     1441 ccttccacgc tccggccttc tgatagtcct cgatctggcg gaacacgtag ccttggctgc
     1501 tcgtccagtc gccatggctt ttctggccga cttcggccac cactttcaaa cctgcatcgc
     1561 cggccattga aatcaaccgg cacttgtctt caagcgccat cgcgacttgc gcgctggaca
     1621 cttccaccgc atccgcgcca aaatccttga ccgcctcata gaagcgacgc gaaacgccct
     1681 gcatgaaggc cgcttcggtg gcgtctccgg caaggaacac accgatgtcg ttctgcttga
     1741 gcatggctat tttccgcctg agcaggtccg ggcgaagaag gcgccaggcg ccgattccca
     1801 tcttgaagaa gtcgatatgg tcgccggcgg tttcgatgat atcggcaagc ctgttcaggc
     1861 ccattcccct gtctgcaagg aaggtcatcc ccgactggcg cggcttcgcc ggcctcgccg
     1921 ccgtgcggat aaaggaaaaa gccagttcgg cttcaacgcc ttgagacatc gatgaaccct
     1981 cctcatcaaa acaaatgctt gccaacactt gtgtcttata taagatatga ttatcaggca
     2041 agcgccttga tggctggtgc gaacgtttcg gaggaaaaat gctggttctg tcgaatgagg
     2101 aagccgaagc gcttttgcac atgcccgaat gcatggatgc gctggagcag acctatcggc
     2161 aggccgccga gggaaccgcg gtcaacggcc gccggtccga catgatcacc tcaacgacca
     2221 ggcccgattc ggtttattgt ctgaagctcg tcggcggcgt ggtgcccgcc ctgaagatcg
     2281 gtgccctgcg tctgaactcc gacatcctgt cgtttcccga gaaaaacggc tctcgccgca
     2341 aggtcaaggt gccggcggcc gccggagacc gctgggtcgg gttggtgatg ctgttttcga
     2401 ccgagacggg cgaaccgctg gcgatcttcc cggacggcgt cgtccagcgc gcgcgcgtcg
     2461 gcgcgacaag cggccttgcc gtgcgccacc ttgcccggcc cgatgcgaaa cggctcgcca
     2521 tattgggcag cggatggcag gccggcgcgc aggcgatggc cgccgccgcc gcccgcccgc
     2581 tgagcgaaat acgttgctac agcccgaacc tcgacaatgc ccgccaattt gccggcgacc
     2641 ttgaaaagag cctcggcatc gctgcctcgc ccgccgcatc ggcgaaggaa gcggtcgagg
     2701 gcgccgacat agtcttgtgc gccaccaaca gcctggctcc ggttctcgat gccgactggc
     2761 tgcaagaggg cgttcatctc ggatcgatcc gcgacaggga actgccggct gcggcaatcc
     2821 tcaaggcaag caaggttttt atccatcacc ccgacaatat gggaagcgat cacctcgtag
     2881 tcgccagcga cgtgacgtat gacgagggcg tgaaagccat tacctccgac cccacgctcg
     2941 aacccttcac ccgcgcgccg acattggccg atctcgtctc cggccgggcg cagggccgga
     3001 ccagcgcgga cgacatcacc tgcttcctca attatcatgg ccttggctat cagttcgcag
     3061 ccgttggcgc ggtgttttat cgcaaggcgc tcgaagccgg ctgcggacat gcccttccca
     3121 ccgagtggtt caccgagaat gtccacccgt gaagaccgca gccgctcccc gaaacatcga
     3181 cgcgagcggc catgatcgag cagacagccc tgcatatcga tgcgctgacc ggattgctgg
     3241 ttgccgcgat ctgctttctg gcggcggcgg tcggcggctt gagcggtttc ggcagcggcc
     3301 tgatcatgtc cgtcgccatg gcgccgctgg ttggcgtgaa ggccgtcgtc ccgttgatat
     3361 cggtagcgat gatcttcacc aacgccgccc gcatctgggt ctatcgggca tcgttcaatc
     3421 ccaggattgc agtcattctt ctggcaacgt cgatcccggc ttccgtgctg ggcgcgatag
     3481 tctacgtcaa tctcgacgcc cgtctggtgt cgatcgtctt cggtttcgtt ctcgtgcttt
     3541 cgatgccggt caaccatttc ctgcggcggc gacgcctttc gctgacgccc tggacgcttg
     3601 cgccgctcgg cctgattttt ggtttcctca gcagcaacat catcggagcg ggcatgatta
     3661 tcccgccgat cctgatcgga tcgggcctgt tggggcctgc ggtgatcgcc agcgacgcgg
     3721 caatcgcggt tggaaccagc gtcttcaagg tgatgacctt cggggccatg gatgcgctta
     3781 ccccgccgct cgccctctat ggggtggtca tggggttgtg tacgataccc ggcacatggg
     3841 cctcggcgtg gctgatggcg cgcacgagcc ttcgtcttca caccgcgctt atcgaggcgt
     3901 gcatcgccgt cgcgggaata aatttcattt ggcaaggctt taacgcggcg tagaacgtca
     3961 cgtattttcc ggtctggtcg aggttaggag gacctcaaaa atgcaaagca acagatttct
     4021 aaaaggcgat acgatcacag cggtcgcact gatagctttc gcggggattt tttgggtggc
     4081 ggccgaccgc ctgcccaaaa gcgccctggg cggcggcgtc ggggctgacg ggctgccgaa
     4141 atttctggcg gtggcgcttg ggctgttgtc gttgatcctg cttgtgcagt ccctgttgcg
     4201 aaaagaacag aagccaacgg aagaaccggg gagcgacgag gacaagccga tttccctgta
     4261 tgcacatatg acctcgctcg gcattctcgt gatcggcgga atttcggtgc tgctgctgcc
     4321 tatcctcggc tatacggcga ccatcgcgct tctgcttgtc gccatggctc tctatcacgg
     4381 ccagaagttc acctggacag tcgccatcta tggcgttctc ggcgcagtcc ttctctatct
     4441 ggtctttgaa aaagtgctgg cggtgccgat gccggaaggc atttggcctg gcctgttcgg
     4501 cttgcagtcc tgaggagggg atgcgatgca aaactttcat tatatgatcg acgccatcac
     4561 gtcgtggtcg gcattgctgg ccgtggtcgt cggtgtcttg tggggtattt gcggcggcgc
     4621 gctgcccggc atctcggcat cgatcgccat ggcgctgctg ttgcccttca ccttctcgct
     4681 ggagccgatg acagcgatga ttctgctttc ctcggtctat atcggcgccg aatatggcgg
     4741 gtccattccc gccatcctga tccgcacgcc gggcaccaat tccgccgcgg ccacggtttt
     4801 cgacggctat gaaatgcaca agcagggcaa aagcggcgag gcgctcggcc tgtcgctggt
     4861 cggtggaacc atcggcagcc tggtcggcct gctcgtgctg atcggtttca ccgaaccgtt
     4921 gtcgaggttt gcgctgctgt tcacgcctgc cgcctatttc gccatcggcc tgctcggcat
     4981 cagcgtcgtc gcctcgcttt cctccgacgc gccggtgaag ggcctgatcg ccggggtcct
     5041 cggccttggc ctcgcaacga tcggaacaga ccccttgtcg ggcgttgccc ggttcacgtt
     5101 cggcagggcg gaattgctgg gcggcatcag tgtcgcggtc gtcatgatcg gcatcttcgc
     5161 ggtcagcgaa ctgatgatcc agcggcaatc cggcgatcat gaggtcgatg ttaccggaac
     5221 gcccccaaaa ctcgtcatac cctcgctgaa gacgctttgg gacctgcgcg ttccccagat
     5281 catcggctgg atcatcggca ccatcgaagg cgcggtgccc ggcgccggcg gctcggtcgc
     5341 cgccttcatg tcgtataatg aagcccggcg ctggtcgaag gagccggaga aattcggcaa
     5401 ggggtcgccg gaaggcgtca tcgctcccga aaccgccaat aatgtcgttg cctcgacggc
     5461 cttgatgccg acattgagct tcggcattcc cggctccaac tcgacggcgg tgctgttggg
     5521 cgggcttctc atccatggat tgcagcccgg tccgctgctg ttttcgaccc atcccgatgt
     5581 cgtctatggc ctgtttggcg cgatgttcat ggcgattgcg gcaatgttcg tgctcggcac
     5641 cgtcattctc ggcccagtca tctggctagt ccgccggccg aaaccctacc ttcagggagc
     5701 gattttcgcc ctgttgatct cgggcatcta ttcgatcgac gacagcgcct tcgacgttgg
     5761 cctgatcctc ctcatcggcc tcgtcagcta tgtgctgcgc gtcctgaagt ttcctacgct
     5821 gccgatggtg ctgggcgtgg tcctcggctt catgatcgaa tccaactatc ggcgctcgct
     5881 gctgatcagc cgtggcgatc acatgatctt cctgcgcgac ccggcctgcg cggtgctttt
     5941 gtgcctcgtc gcgctgatgc tggtcaccag cgtatggcgg gagatgcggc cgcggaagcg
     6001 gaccccgaag gaagcatgaa gaaagggcgc ccaagcaggc gcccttttct tttcaatcga
     6061 atttcccggt ctggacggaa cgaccgagcg cacaaaattc tcgctgtcta ttgcgggaac
     6121 ccgtgttcat ggacaacggc ccagatcttc ttgttcacat cggcgatatc ctcgatgcaa
     6181 tcggatatgc gggcagcttc ggccgcgtcc aaagcctcga ccttgccgta tttgatcgaa
     6241 tccttcccgt cgaatatgcg catcagctgc gtgtgcgaaa tatcgccctt gtcgtcgaac
     6301 gaataaatgc agtggttgta tttgttgcgc aatttagact gctggctcaa ccgctccaga
     6361 agttcaagaa tatccctctt gcacttctcc ggcacccttg ggagctttgc cagcctttcc
     6421 accaggtcta tccttgccct cgtcgtgtta agggtaagga agatgacgat ggccgtttcc
     6481 ttgtcgacgc gggcgagacc cgcgatcaga tggatgagca ggctttcggt attggtccat
     6541 gtgtagttga gccggccgat caaaagcagt atctgctgga aactctgcat ttcaggacct
     6601 cggcggggca ggacctcagc ggcccttggc atggccgccg ccaatcctat ccagaaacca
     6661 tcgaggacgg caacaacctt cacggttgcg gcccgcaagc atcatccaaa cggatcaacc
     6721 acgcctttgg aggtatgggc ttcgctgcat ttttcgccgg caggcgtagg tccgatacct
     6781 tggcgctggc gatctagtca gaatgtgcag ctaggcgccg gccaccctct tcgcccagta
     6841 tcggattgtc tcggcagttc cgcgacacgg acatgctcct gatttcagcg acttcatttg
     6901 ccggcctgac attcgtttgg ccgggcgcgt cttgatgtgc tgaacgatgg cgctggccgc
     6961 tcaccgggga ggaatggatt tcagtagagt atcagatcga ctggccgcct tgtcgtgagt
     7021 acggccactc cagttctcct catttgtgca accgcccata cggctcaagg ccctttgaca
     7081 gatgaacagg gccatgccgg aacggcttcc agcatgaggt aacactatgt cccctagaaa
     7141 ctcgcacagt tccagccctt atatactcga tcccagccag gaccagagcc aggaccagtc
     7201 gcagggcgaa gcccaggcgc agctccaggc cgaacttcag ggtcaaggcc agggccaata
     7261 ccagtccagc cattccgaaa atgaaaacgg caatggcaac ggcaacttga atggcaatgg
     7321 caatctcaac ctgaacggaa acgccagcct caacggcaat ctcaatgcaa acctgaacag
     7381 gtcgatcaac gagagcacca atacgaattc gagcaccaac acgaattcga ataccaatac
     7441 gtccgacacg catgtgaatg tcgacgttgg agtggacctc accggctacg agccgagcaa
     7501 ccgcgacgtc aacaacatca agctcgacaa gggcgccgag atcgacaagc tgctcgtcaa
     7561 ccatgacggc cagatcttct acaatccagg cgacggcgcg acgttcaacg atttcctgaa
     7621 cggctcgctg accggcgaag gcaatgattc cgccctgtcg gttgcgcagg tcaacaatct
     7681 cgacgacagc gaccatatcg gccgcgcctc cgtgtcgaac gacggcacct tcgaacagaa
     7741 cggcgatgcc cagggcggcg acgcttcctc cggttcgggt atcaccgccg gtggtggcgg
     7801 cggtggcggt ggtggccatg gcatggcttc cggtggcgat ggcggcaatg gccacagcct
     7861 tggcggcatc ggcatcggcg gcagcgccct gggtgcggcg cgcggcggcg atgcaacggg
     7921 cggcgcgggt gctgccggcg gtgctggcgt aggcctcggt ctcggcctcg gcgcgggcat
     7981 tggtgaaggc ggcgctgccg gcggcgatgg cggcaacgca aacggcggcg attcgaccgg
     8041 caatggcggc gcaggcctcg gggctgggct ttccggcgcg ggcggcggcg acagcggtaa
     8101 tgccgacagc ggcgatggcg gcaatgccgc cggtctcggc cttggcggcg caggcagcgg
     8161 cgatgccggc aacggcgaca gcggcaatgg cggcgacgca ggcggcctcg gcattggcgc
     8221 cggccttggt gacggcggca atgccggcga tgcatccgcc gatggcggcg gcgcgcttgg
     8281 tctcggcctt ggcctgatcg gcgttggcgt cggtgtggcg ggcgatgccg gacaggcaac
     8341 cggcggcgct gccgatggcg gcgcgggttc aggctccggc agcggcgcag gcaccggtgg
     8401 cgacagcggc aacgccaatg gcggcgacag cggcgatgcg accgggcttg gccttggcag
     8461 cggccttggc ggcgacagcg gcaacgccaa ttccggcgat gccggcgatg cgctctcgct
     8521 cggccttggc cttggccatg gcggcgatgc cggcgacggc ggcctcggcg gcatggcggg
     8581 gcttggcggc gatgcgctgg gtctgggcat cggcaccggc tttggtgaag gaaccggtgc
     8641 cggcgtcggt ggcgcaggcg gcatgggcgg ctttgcctgg agcggcgata ccatcgtcgt
     8701 gccggtggcg ggcgacggaa ccggcggtgc ttcttccggc ggcgcaggcg gcatgggcgg
     8761 cgacggtggc attgtcacca atggcgctgg cggtgccggc ggcgacggcg gcacatgggg
     8821 aaccaacaac ggccatgaca gctacgtcaa cggcctttcg gaagcccatg cggacgccgt
     8881 catcgacacc agcgccttta accaaagcat cgtgatgggc gcgaacatga tggaaaacct
     8941 gctcagccag accgtcgttg gcggcgatta cgacaatatg gaccatagcg gctcctgacc
     9001 gccgcaccgt tccgcgcaag cggaacctga ccttcgggct acgcgggctt tctcgcgtag
     9061 cccatgaaaa atggagaatg ccgcagccca accggagagc gctcccgtgc atttggacaa
     9121 gatcaccgaa gcaatcgccc atttcgtcgg gctgtttcag atagagaccg agcaggcgcg
     9181 gctgcgctcc gattatctcg aattcaaagc tttgcgggcg caagcgaccg ctcccgatca
     9241 gcacgaccgc gccgagctta ccttccaatc gccttataat ttcaacgatc cggaccccgg
     9301 cctgcactat gtacccgccg ccccgaccat cgaaccgctg acggttgcca ccaacgtgac
     9361 ctatgcgatg ccgcaaattc ccgtaccggg tgagatcgag gccgtcatct accccggttt
     9421 catgacaccg cgacttctcc agcaagacct gcacaccggc acaaggatca tcgagcttgc
     9481 gccgccgggg tcggttgccg tgcatatttc acagatcaac agcctttccg acaatgattt
     9541 cgtcaatatc ggcaccgatg ccgtggcctt ccactcgatc ggcacgccca acctgacgct
     9601 cgatacgttg ctcagcgaag cggcgaaatt cgttcctatc gaggctgcca cattcgcgtc
     9661 ggcgcacgac ataggagact ttatcgccga taccggagcg tcgctgcacg catttgccgc
     9721 gcaggcgaat ggctcgacca ccgaaaccgc cgatggcgta tccatccaga cgaatgtcac
     9781 ggtgatcgac gcgccggaaa acaatgcggt ctacgtcaac ggtcacatgc aatccgaagc
     9841 gccgaagctg gaggacgtgc ttccggcggc gtcgcctctc gtcaaggacg atgtggccac
     9901 gcccgacgat gcgcccggca ataatcacct gatcaacggc cccggctcga ccgccaccgg
     9961 cggcgtcgcg catggggaag gtgcggcttc gaacgccagt tcggtggaaa tcagcaccgg
    10021 cgccaacgcg ctggtcaaca ccgcaacgct ggtcaacgat gcgatcgagg gcaatgtctt
    10081 tgcggtggcg ggcaatcatt tcgcgctgaa cgccatcatc caggtcaatg cctggagcga
    10141 ttgcgattcc atcggcgcca gcctgaacaa ctgggatgcg gcaggccacg cagccgccac
    10201 ggccacgttc aatatcgcat cttttcagca catcgacacc ggcggcgacc tgccgggaac
    10261 aggcgacccg gcgcagatga ccttccccaa ggcctgggcg gtcaccgaaa tccacggcga
    10321 tttcgtttcg atgaactgga tccagcagct taatttcatg atcgacaacg acaatgtcgt
    10381 ttccgcctcg tcgtccggcg tgatgacgaa gctgggcacc ggcgagaacc agggcctgaa
    10441 cgatctctcg atcgccgatc ttgggcggta ttacgatctc atcttgatcg gcgggcatta
    10501 ttacgacgcc aacatcatca cgcagaccaa cgtcctgctc gatgacgata ttgtcggggc
    10561 ggtttccggt ttccatacat cgggccaggg ttcggtgtcg ggcagcgaca atcttttgtg
    10621 gaacagcgcc gccatcacca gcatcggtca cgacacgacc ggcgcgctgc cgaccggata
    10681 tagcacggcg ctcgacggct ttgcggccgg caaccatacg ctgccttcga gcgtgctcgc
    10741 ggacggcgcg ttcgaggggc tttcgggact gaaggtcctc tatatttcgg gaagcgtcta
    10801 cgacctgcaa tatgtcagcc agaccaatgt tctgggcgat gcagaccaca tcgcgctggc
    10861 gatgaattcg gccgaggcca acctcgatgc cagctggacg atcacgaccg gttccaatgc
    10921 gctgctcaac tcggcccaga tcatcgatgt cgatccggcg ggcaagatct atgtcggcgg
    10981 cgatcactat tccgacgagc tgctggtgca gaccgacatc atccgcaccg accatctgct
    11041 tgaaggcaag gacgccgacc atctggtcaa cgaggccgtc gcctttctca gcgacgacat
    11101 gatgccgacc caggccgatg atgcgtcgtc gcacctgcgc gacgccgtca gcgtgcatcc
    11161 cgcccacagc gatatcatgc aatccgtcgt gtcctgattt caatatcgga gcggtaatga
    11221 acgagcacgt aacctccacg caacagacgc cgccagccgg tgacggacct gagccggatc
    11281 atgtccgggg gccgacaccg aaagccgcct cgtcgctgaa ggacatcgag gtttcgttcg
    11341 aagtgctgat ggcggaaatc gatcaagccg cagccgaact gaaacgcgga tcgcgtccga
    11401 tcttctttcg cccgctcgat acggacctgc cacccgaccg cggaccagcg ccctctgcgc
    11461 cgatggaaat cgaccagccg gcggttaagg gcggtgcatc tgggagcatt gcggcaggcg
    11521 cgctgaagtc gatcgacggc gacagcgggc cgatcaaggc gagcgatgca cccgcgatac
    11581 aggtgctcaa ggaacgcggc aaggagccgg ccagcaccat cagctcgccc agcgccgcca
    11641 acctgcccag ccccaccagc caggtgtttc accgccggct gggttcgatc aaccttgccg
    11701 acaacctcaa cgcgggaatc cacgccgtac ggcgcaatct tctggtggtg atgggcttca
    11761 cgctcgccac caacgtgctg gtgctggcaa tcccggtcta tctcttccag atatccgacc
    11821 gcgtgctgaa cagccgttcg accgacacgc tggtaatgct gacgatcctg atcgcggggg
    11881 cggtcattct caacgccgtg ctcgacgcca tccgccgctt catcctgatg cgcacagcgg
    11941 tggaggttgc cgcccagctt ggcgcgccca ttctgggcgc ggccgcgcgc gcctcgctca
    12001 acagcaacgg gcgcgaatac cagacgctgg gcgacctcca gcaattgcgt gccttcctgg
    12061 tatcgggcac gcttttgtcg ttcctcgacg cgccgcttac gccgctgttc atgctggcgg
    12121 tgttcctcgt tcacccccac ctcggctata tcgtcgtcat ttccgccctg ctgcttctgg
    12181 tcatcgcctt catcaaccag cgggcgacgc aagcgccgtt ccacgaagcc ggcaacttcc
    12241 agagcagggc gaaccttcac ctcgattcca tgtcgcgcaa ctcgcagatt atcaacgcgc
    12301 tggcgatgat cccggaagcg gtcaagatat ggggccgcga taccgccggc gcgctgaagg
    12361 cgcaggtggt ggcgcaggac cgcaacgtca tcgccgcttc ggtatcgaag gcggtcagga
    12421 tgctggcgca ggtcgcgatg ctgggctggg gcgcgtatct ctcgctcgaa ggccagttga
    12481 cgggcggcat ggtgatcgcc gcgtccatca tcgccggccg cgcgctggct ccggtcgaag
    12541 gcgccatcga gggctggcac cagttcatca atacccggtc ggcctatcag cgcatcgctg
    12601 ccctgttgcg cgcatcgccg ttgaatttcg agcggctgcg gctgccgcgc ccgcaaggca
    12661 ggctcgacgt ggagcggctg ctgttcgtgc cgcagggcac caagcgcgtc gtcttgaacg
    12721 gcgtcacttt caccctgtcg cccggcgaat cgctggccat catcggcgac agcggagccg
    12781 gcaagacgac gctcggcaag atgctggtcg gctctatctt gccgacatcg ggcaatgtgc
    12841 ggcttgacct gatggatttg cgcaactggg accagcgcca gttcggcgag agcattggct
    12901 atttgccaca ggacgttcag ctgtttcccg gcaccatcaa ggccaatatc gggcgcatgc
    12961 gcgacgacgc cagcgacgag gacatctaca aggccgcgat gctggccgat gttcatgaac
    13021 tgatttcgac ctttgcgcag ggctatgaaa cgaccgtggc cgccgatggc tcgccgctgt
    13081 caggcggcca aaagcagcgc atcgcgctgg cgcgcgcctt tttcggcaat ccgcgccttg
    13141 tcgtgctgga cgaacccaat tccaacctcg acggcaccgg cgatgccgcg ctgctgcgcg
    13201 cgcttgccca tgccaagcag gaaaagatca ccgtcatcac catcacccag cgcccgtcgc
    13261 tgctgaattg cgtcgacaag atcatgctgc tcgccaatgg cacggtgtcg atgttcggcg
    13321 tgcggcagga cgttctcaag gcgctcgcac ccctcaacac cgccaatgcg cgcaacgcgg
    13381 ttggccacca gccagcacag gcgacgccat gaacaccagc attgccgatg tccacgatat
    13441 cgaatggtac ggccaggtgc cgcgctcgat ccggacgcac gctttcgtcg gcctagccct
    13501 catcgtggtg gcgttcggcg gcttcggcac atgggcggcg accgcgccgc ttgccgcggc
    13561 cgtggttacc gccggcagct tcgtggcgac gggcgaaaac aaggtggtgc agcatctcga
    13621 aggcggcatc atcgacgaca ttctcgtcag cgaaggcgat cacgtcaccg ccggcgaacc
    13681 cctgatccgc ctcgacggaa tcgccgcgca ggcaaagcag cggcagttgt tcctgcgcca
    13741 ggccaggctg gaggcgaatg tggcgcggct ggaagcccag atcgacgcca aatccgagat
    13801 cgcctttccg acgatcatca ccaacaatgc cggcgacagc gacataaagg cgatcatcga
    13861 cggacaattg gccagtttcc gctccgcccg aaaccgtatc gacagcgaaa cggggcttct
    13921 caagctcaac atggagtcat tgaaattccg gaccgagggc tttcagcggc aaagcgcatc
    13981 gatggccgag cagctcgact atctgaagca ggaatatgcc ggcaagctcc agctcctggc
    14041 tcagggcctt atccgcaagc ccgaagtcaa cgccatccaa cgcgccatag ccgacgcatc
    14101 ggggcagata ggccggctgg attcggaaat gtcggaaacc aattcccaga tcgagaaatt
    14161 ccaccagcag atcaaccaga cggtaacgga actgacgcag aaggcgctcg acgaacttca
    14221 aggcagccag ggcgacctcg atggcgtccg gcaagaggcg cggcaggcga agaacatcac
    14281 gcaacgttcg acgatcaacg cgccggtttc cggcacggtg gtgcggatgt attatcatac
    14341 gtcaggcggc gtcatcgaaa gcggcaagaa aatcctcgaa atcctgccct ccgacgtgcc
    14401 gctgatcatc gagacgcaaa tccagcggaa agacatcgat gcggtaaagc ccggccaaaa
    14461 ggcgacggtg cggctggtgg cgctcaaccg gcgcacgacg ccggtgctga acggcgatgt
    14521 ctattatgtt tcggcggacg cgttgtcgga cgcgacaatc cccaaccagg aaatctacct
    14581 ggcgcgcgta cggctttcgc ccgcagagct tgcccgcata cccggcttca tcgcaacgcc
    14641 cggcatgccc gtcgatgtga tgatcgaaac ggcggagcgt accttcctcg actatctgac
    14701 caagccgatc cgtgaaagca tgtcgcgcgc cttcaccgaa cgataagggc ctttcgccag
    14761 gccggcagac aagagaagcc aggattgcga tcaaagcctg acggcgacgg gtcgcgtctc
    14821 ggcgaggtag gaggccgcca ccgagggcag gctcgccgag gtcaattcat atatgccgca
    14881 attggcgatg agcgcgcgta tgtcggtgct caacccgcga tggagccaga tggtgcgcgt
    14941 cgcatagggt tcgttgccgc ttcgccatga cacgcccatc gtcagcccgc gcagatattt
    15001 gcgctggtgg atttcgaacg gcatcaggat ggtctggccg aaagtcggct gctcgccgcg
    15061 cgtaagctcg gtcagaaaga tgcgctgacg ccggaaggcg gccagcccga catagcgcga
    15121 acgttggcgg atgccgcttt gcacatcctc gatgcgttcg agcgacgtca ccagcatctg
    15181 gccgttcctc tccctcaaat gggaacagga acagacggcc cggccgggcc aggaaagcga
    15241 tatgtgccag gtctgataga acccgacatg cggacgcaac gccggcaagt cgccggggaa
    15301 cgcctccgcc aatggcccct ccgccatcgc cgagggacgg ttttccttca gcttttgccg
    15361 aaaaatatgc gccggcaggt caaaatcgtc cgcttcgagg tcaaatgcgg cggcgatcct
    15421 gagacggttg tgggccgatg gcagcgcgcc ggcattgatg tagcggttga actgctgccg
    15481 gttgatgtcg atcgtccggc aaacatcgga gatcgactgc ctggtgctac aggcaagctt
    15541 cagattggcg gtgaaattgg aatgggagtt acgcattcat gtagcgtaga accgtataaa
    15601 agcgcgtcaa gccgtcaaat tgcgcgcggg caaaccgggt ccacaatcgg cgaaataatg
    15661 ccggcaaaaa ggggaacaaa ttgaccgatt tcgacaacgc attcagcctt tcccgtcgtg
    15721 gcttcctaca gggaagcgcg cttctggggc tgtcggcgac taccgggacg cttgtcgctt
    15781 cgcctgcgcg agcgcaaacg ccggtcaagg gcggaacgct gcggatgggc cttggcggcg
    15841 gcgcctcggc cgacacgctc gaccctgcgc tggcgaccgc cgccgtcgcc ttcatcgtcg
    15901 cgcattgctg gggcgacacg ctgctcgaat cccacccgga aacgggcgag ccgctgcctt
    15961 cgctcgccga atcctggacg ccttcgcccg acggcaagga atggaccttc aagatccgaa
    16021 agggcgtcag ctttcatgac ggcaagccga tgacgccggc cgatgtggtg gcgacgctgc
    16081 gccgccatgc cgacgccggt tcgaagtccg gcgcgctcgg cctgctctcc gacataaagc
    16141 agatcgagga gcgcgccggc gacgtggtca tcacccttgc cgagggcaat gccgatcttc
    16201 cgctgctgct gaccgattat catctgcaaa tccagccgga aggcggcacg aagaacccga
    16261 ccgcggcaat cggcaccggg ccttacaagc tggttgcctt cgagccgggc gtgcgcgcgg
    16321 ccttcacccg gaacaccaat gactggcgcg acgatcgcgg ccatgtcgac agtgtcgaaa
    16381 tcacggtcat gaacgactcc acggcgcgca tcaatgcgct gagcgccggc caggtcgact
    16441 tcatcaacac ggtcgagccg aaggtcgtgc cgctcttgtc gaaggcgccg aatgtcgaga
    16501 tcctgcaaac gtccggcaaa ggtttctact cgttcctcat gcattgcgac accgccccgt
    16561 tcgacaataa cgacctgagg ctggcgctaa aatatgcgat cgaccgcgag gctatcctca
    16621 agcgggtcgt tggtgggttc ggcaccatcg gcaacgacta tccggtcaac gccaactatg
    16681 cgctggcccc gaccgacatc gagcagcgcg tctatgatcc cgacaaggcg gcgtttcact
    16741 tcaagaaatc cggccatgac ggctcgcttc tgctgcttac ttccgaagct gcgtttccag
    16801 gcgccgtcga tgccgcgcag gtcttccagc aaagcgcggc caaggccggc atcaagctcg
    16861 acgtccagcg ttcgccaagc gatggctact ggtcggaggt ctggaacgtc aagccgttct
    16921 gcgcttccta ctggggcgga cgcccgacgc aggattcgcg ctattccacc tcctacgtct
    16981 cgaacgccga atggaacgat acccgcttca agcgcccgga tttcgacgcg atggcgatgc
    17041 aggcgcgcgc cgagttcgac caggacaagc gccgcgcgct ctaccgcaag atggcgctca
    17101 tggtgcgcga cgaaggcggg ctgatcctgc cggtgttcaa taatttcgtg aacgcttcct
    17161 ccaagcacct gaagggcttc gtccacgata tcggcaacga cgcctccaat ggacgcatcg
    17221 ccagccgcgt ctggctggaa agctgacgtc gcactgatcc tgcctgtctt tcaagcgcgg
    17281 cggcataccg ccgcgcgcca ccgcctgctg gaaaaaatct catgccggat atgaattttc
    17341 acatcatcga aaacgagtgg atcgtcctga aagacggcac gcggctggct gcgcgcatct
    17401 ggatgccgga cagtgcggcg gcaggagcgc cagtgccggc ggtgctggaa ttcctgccct
    17461 accgcaagcg caacggcacg gcgccacgcg atgaatccac ctatccggtc tttgctgccg
    17521 ccggcattgc gggcgtgcgc gtcgacattc gcggcagcgg tgaatctgat ggcgtgatcg
    17581 acggcgaata cacgccgcgc gagcttgccg acgcggtcga ggtgatcgag tggatcgccg
    17641 ctcaaggctg gtcgaacggc aatgtcggca tgatgggcat ttcctggggc ggcttcaact
    17701 gcctgcaagt ggcggcgctg aaaccgccgg cgctgaaggc ggtgatctcc atcgcctcaa
    17761 cggtggaccg ctacaatgac gacatccact acaagaacgg cacccatctt tcggcgcatt
    17821 tttcctgggc ggcgacgatg agcgcctacc agtcgcgctc gccggacccg gagatcgtcg
    17881 gcgagcgctg gttcggcatg tggctggagc ggctggagca ggagccgttc ttcatggaag
    17941 aatggctttc gcaccaacgt cgcgacgcgt tctggcggca cggctcgata tgcgaaaact
    18001 tcggcgaatt cccggttccc gcgctggtga tcgccggctg ggcggacggc tatcgcaata
    18061 cgccgctgaa ggcggtgacg gggatgcccg acaaggcggt cgcgctgatc gggccatggg
    18121 tacacaaata cccgcatttc gcctacccga aaccccgcgc cgacttccat ggcgaggcga
    18181 tcgcctggtg gcggcgctgg ctgtgcgggg aacaaaacaa agttgaacag acgccgcagg
    18241 tgcgggcctt tattctcgac gggccgaagc ccgcaggctg gcgcgaaacc gacccaggct
    18301 attgggtcgc caaggagaaa tgggccaaac cggaagtcgc cacgctcaat cccagcgctt
    18361 cgggtcaact cgaattcaat ccggcaaccc gcgctagcgg ccatgcgctg ttgcgttcgc
    18421 cgctcgacac gggcacggcg gccggcagct tcttcaccgt caaaccggac gccgagttgg
    18481 ccggcgacca gcgcctggac gatgccggct cgctgttttt cgacagcgag ccgcttaccg
    18541 aagaatgtgt tattctcgga cagccggctt tcaaggcaag gctttccagc gacgcgccgc
    18601 tcgccaatct ggcggcgcgg ctggtggaca ttcatccgga tggtacggct acccgcgttt
    18661 ccttcggcgt gctcaacctc gcccaccgcg ccggcagcgc cgaaccgcag ccgcttgagc
    18721 cgggcaagcc cgtcgagatc gaaatggcgc tcgatgcctg cggttatcgc ttcgcgccgg
    18781 gccatcgcat ccggctggcg ctttccagcg cctattggcc gatgatcctg ccgccgccct
    18841 acaacgcggc gctggatttg gacctcgcca cgctcaaact cgagcttccg cttctgggtg
    18901 cgcatcgcag ggtcgaggtg gccgagccat ccaatcccga tccgctgccg cgctacgaaa
    18961 ctctttccaa agggtcgtcc gcccgctccg tcgaacgtga cctggcgaac ggcgtaacgc
    19021 gctaccggat cagcgaggat ggcggcctca accggcatcc gggcaacggt ttgagcacgc
    19081 gcgacgccca cgagga
//
LOCUS       DASYCS010000077         6006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_596520,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000077 DASYCS010000000
VERSION     DASYCS010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 6006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6006
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596520"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VGM46_02730"
     CDS             complement(<1..371)
                     /locus_tag="VGM46_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HEY4191538.1"
                     /translation="MSQTIEAIRDDFALLDEWEDRYRYVIELGEALPSFPDSERTATN
                     KVPGCVSQVWLTTGTGTGSDPVMTFQGDSDAHIVRGLVAIMLALFSGQKASTILETDA
                     DAVLKTLGLDEHLSPQRANGLR"
     gene            complement(481..801)
                     /locus_tag="VGM46_02735"
     CDS             complement(481..801)
                     /locus_tag="VGM46_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010915016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5330 domain-containing protein"
                     /protein_id="HEY4191539.1"
                     /translation="MFFLMRMAFWFSLVLLALPFDTSSDNAGQPSVGAIQALVAARQA
                     VGDISGLCERQPEVCETGKSAMHTVTVRAIKTAKFAAEMLDEDTPAAQPDKSTTTGSI
                     AAHN"
     gene            1304..3052
                     /locus_tag="VGM46_02740"
     CDS             1304..3052
                     /locus_tag="VGM46_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018426962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEY4191540.1"
                     /translation="MSVGCERLVHPSVQDAGEHSRQRRFIGVMLASPFFIAGAAVTLI
                     AAGMGAAVTVAAIFAAFGLCWFTALLVAASASTKAAAPLALIVAGAVSALLIAAGGGF
                     ASPVALMVLALPFEAFWIAGTRKAAFWGAAGALGAVLLQPVAAALLPPGSGVSAWNWL
                     LPLAWAATIALRHNGLRAMAAMQDAPESAPLLEDMLDAVVFRTGRQGEVLDVSEKARS
                     ILNLAPELLAGSGLFDRIHLSDRVSYLSALADLREGAVLRKLDLRIRLPQAGSAGIVG
                     NYRRFSLELMRAQNEDGVFIALLREGGVVADLREELDRANEAAASAEIAKGRFLAAVS
                     HELRTPLNAILGFSDMLLHEMFGSFNDPRQKEYVGLVRDSGQHLLDVVNSILDVSKIE
                     CGAYTTEPEPFRFIEAVEMCRSMMQLQAQAKTISLATQMTPDLGEINADRRAVQQILI
                     NLVSNAIKFTHDGGDVVIGANKIGSRLHFWVSDTGIGIAEENIASLGKPFMQIQNGYT
                     RAFEGAGLGLSLVKGLVALHDGTMSVESAPGEGTKVTISLPIDGPIERPEKSAVVPMT
                     ARKTKEDGNGSLRKTA"
     gene            3027..3800
                     /locus_tag="VGM46_02745"
     CDS             3027..3800
                     /locus_tag="VGM46_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019857118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HEY4191541.1"
                     /translation="MGRSAKQRKKPVRRSQGMLKEGAVALGGMISDNPVLVGGSTAFL
                     VTFFFVSANALWYQPYPHTGAFFATRAIQGFSPPQIDQPETTINIERPAQSSAPPAAD
                     PVIAKVQGILGDLSFYSGAVDGIQGPNTHKAVEAYQRKMGLTPTGNINSQLLDQLGAT
                     TGATAVPQPAPRADVTNATQPDATAPKETASAGADARIVKIQAGLKAFGNDGIQLDGV
                     VGARTVAAIREFQSLFGLPVTGQPEEAVYAKMREIGLTN"
     gene            3885..4232
                     /locus_tag="VGM46_02750"
     CDS             3885..4232
                     /locus_tag="VGM46_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010915019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1491 family protein"
                     /protein_id="HEY4191542.1"
                     /translation="MRVTADLWVSALVRRVFSAGGFAAVVKRGATEAGAVFVLSRDRM
                     GEVTLYGPAPQTSYDSAKPDDRHFLIVGAGEAADALDARLEREKRFDPDIWVVEIEAG
                     SVPVEELISLKTP"
     gene            complement(4219..5169)
                     /locus_tag="VGM46_02755"
     CDS             complement(4219..5169)
                     /locus_tag="VGM46_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HEY4191543.1"
                     /translation="MFRAAISAFCSLTRPSRREIAQIDDLALPLFDGVSVESRRFVAA
                     ALSECEYPPPALVRRLCDEPVDIAAPLLIRSHALGDVDLIALIGRHGLPHARAIARRK
                     GLTPAIADLIRVLEKPTLVRTNTPAENVAGPVEAAPAKADAPAPAGHAAETARHRLRE
                     VMADYEHIVGYEHKGTANINAPDGSTYVKLRETALTGNAAFFQTALADALGVDFATAR
                     SLTESTNYSSLLVALRALDVGEDKAFLVTVAAFPSLFPHPQAIRVFLDRYRTLHHDLA
                     RQKVAEWKAASLSNSLHGIVHTPANADTPAPGRDRKSRAS"
     gene            complement(5661..>6006)
                     /locus_tag="VGM46_02760"
     CDS             complement(5661..>6006)
                     /locus_tag="VGM46_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HEY4191544.1"
                     /translation="AACRFNLNDGMVQLKGPPAAVPFWSVSVYDRNGHNIYSFNDHGA
                     PRGRLDAVVLTPGQMIEVRKDLPPEYQGSVFVEAPIAEGIVVVRSFVPDETWKPAVMR
                     FLGHSSCTLRQP"
BASE COUNT         1077 a         1850 c         1930 g         1149 t
ORIGIN      
        1 cgcaggccgt tggcgcgctg cggagaaaga tgttcgtcta ggccaagcgt cttcagcacc
       61 gcatcagcgt ctgtctctag aatcgtgctc gccttttgcc cggaaaacag ggcgagcatg
      121 atggcgacga ggccgcgcac gatatgggcg tccgaatcgc cctggaaagt cattacgggg
      181 tcgctccccg tacctgtccc ggttgtcagc cagacctggc tgacgcagcc cggcaccttg
      241 ttggttgccg tccgctcgct gtcgggaaaa gaaggaagcg cctcgcccag ttcgataacg
      301 tagcgatagc ggtcttccca ctcgtcaagc agggcaaagt cgtcgcggat cgcttctatc
      361 gtctggctca tcaggctgga tataggcgcg gggcgaagcc gcgtcacgga aaaaagacac
      421 cgcaggcaga gagggctgac gcctgcggtg tcttgcgaat ccgatcgcag aactggtcgg
      481 tcagttatga gcggcgatgc tgccggtcgt ggtggacttg tcgggctggg cggcaggcgt
      541 atcctcgtcc agcatttcag ctgcgaattt cgccgtcttg atggcgcgca ccgtcaccgt
      601 atgcatggcg gacttgccgg tttcgcaaac ctcgggctgg cgttcgcaca ggccggaaat
      661 gtcaccgacg gcctggcgcg ccgccaccag cgcctgaatt gcgccgacgc tgggctgtcc
      721 ggcattgtcc gagcttgtgt cgaacggcag tgccagcaac accagtgaaa accagaacgc
      781 catcctcatc agaaaaaaca tgatacctgc cctcgtcgtg accgggcctt gctcgtgtgg
      841 tggcgaggcc tcgtttgtag gcacgacatt ggcacgcgcc gccaaaaatc ggattgcgcc
      901 aaaacacggg atttgcttca aattttatgg aaattggaaa cgtcctgttt ttgtgctgtt
      961 ttatatcgat atttagcgcc gaaattaact tttagataac tatataacta gctgatacta
     1021 cacggcattt tcgatctctg tagttgtgga cagggtccgg gcggcgagag gcgagcgccg
     1081 ccggcggata accctccgtt tactatggct gcaattggcg gccgctttcg ccgcaagcgt
     1141 gtcgattatc gccttgtgcg cgccgcccga cgcccccgct ttatccattc cttaaaggct
     1201 ggatgcgagt ttcgcagtta accaaagcga tctgccaagc agacaagtgc gagtgcgtta
     1261 cgtgagttcg attgccgcca gatacgttga gttgcccgac gcgatgtccg tcggctgcga
     1321 acggctggtc cacccctccg tgcaggatgc tggcgagcac agccgccagc gccgcttcat
     1381 cggcgtcatg ctggcaagcc cgttcttcat tgctggcgcc gccgtcacgc tcattgccgc
     1441 cggcatgggc gcggcggtga cggtcgcggc gattttcgcg gcgttcggcc tttgctggtt
     1501 taccgcgctg ctggttgcgg catccgcttc gacaaaggcc gccgccccgc tggcgttgat
     1561 cgttgccggc gccgtatccg ctttgctgat cgcagccggc ggcggcttcg cttcgccggt
     1621 cgccttgatg gttctggccc tgccgtttga agcattctgg attgccggca cccgaaaggc
     1681 tgcgttttgg ggcgcggccg gcgcgttggg cgcggttctg ttgcagccgg tcgcggccgc
     1741 tttgttgccg ccgggttccg gtgtttccgc ctggaattgg ctgttgccgc tggcctgggc
     1801 cgcaaccatc gcgcttcgcc acaacggttt gcgcgccatg gccgccatgc aggatgcgcc
     1861 ggaaagcgcg ccgctgcttg aagacatgct ggatgccgtc gtgttccgca ccggccggca
     1921 gggcgaagtg ctggatgtct ccgaaaaggc gcgatcgatc ctcaaccttg cgccggaact
     1981 gcttgccggc agcggccttt tcgaccgcat tcatctgtcc gatcgcgtct cctacctgag
     2041 cgcgctggcc gatttgcgcg aaggcgcggt cttgcgcaag ctcgacctgc gcattcgact
     2101 gccgcaggcg ggatcggccg gcattgtcgg caattatcgc cgcttctcgc ttgaattgat
     2161 gagggcgcag aacgaagatg gcgtcttcat tgccctgttg cgggaaggcg gcgtcgtcgc
     2221 cgacctgcgc gaagaactgg accgggccaa tgaagccgcc gccagcgccg aaattgccaa
     2281 gggccgtttc ctggccgccg tcagccatga actgcgcacg ccgctgaacg ccatactcgg
     2341 cttctccgac atgctgctgc acgaaatgtt cgggtcgttc aacgatcctc gccaaaagga
     2401 atatgtcggc ctcgtccgcg attccggcca gcacctgctg gatgtggtga actcgattct
     2461 cgacgtgtcg aagatcgagt gcggcgccta tacgacagag ccggagccgt tccgcttcat
     2521 cgaggccgtc gagatgtgcc gctccatgat gcagttgcag gcgcaggcga agaccatcag
     2581 cctggcgacg cagatgacgc cggatctggg tgagatcaat gccgaccgcc gcgccgtcca
     2641 gcagatattg atcaacctcg tctcgaacgc catcaagttc acgcatgatg gcggcgatgt
     2701 ggttatcggc gccaacaaga tcggctcgcg cctgcatttc tgggtcagcg acaccggcat
     2761 cggcatcgcc gaggagaata tcgccagcct tggcaagccc ttcatgcaaa tccagaacgg
     2821 ctatacgcgc gccttcgaag gagcgggcct tggcctttcc ctggtcaagg ggctggtcgc
     2881 cttgcacgat ggcaccatgt cggtcgagag cgcgcccggc gagggcacga aagtaaccat
     2941 cagcctgccg atagacggac cgatcgaacg gccggaaaaa tcggcggtcg tgccgatgac
     3001 ggcgcgcaag acaaaggagg acgggaatgg gtcgctccgc aaaacagcgt aaaaagcccg
     3061 tccggcgcag ccaaggcatg ctcaaggaag gcgcggtcgc ccttggcggc atgatttcgg
     3121 acaacccggt gctggttggc ggctcgactg cctttctcgt cacgttcttc ttcgtgtcgg
     3181 cgaacgcgct ctggtatcag ccctatccgc acaccggcgc gttttttgcc acgagagcca
     3241 tccagggctt ttcgccgccg cagatcgacc agcccgaaac cacgatcaac atcgaacggc
     3301 cggcgcaatc gtcggcgccg ccggcggccg acccggtgat cgccaaggtc cagggcatct
     3361 tgggcgacct cagcttctat tcgggcgcgg tcgacggcat tcagggaccg aacacccaca
     3421 aggcggtcga ggcctaccag cgcaagatgg ggctgacgcc gaccggcaat atcaacagcc
     3481 agctgctcga tcagcttggc gcgacaacgg gcgcaaccgc cgttccgcag ccggctccgc
     3541 gcgccgatgt gacgaacgcc acccagcccg atgcgaccgc gccgaaggaa accgcgtcgg
     3601 ccggtgccga tgcccgcatc gtcaagatac aggctggact gaaggcgttc ggcaatgatg
     3661 gcatccaact ggacggcgtg gtcggcgcac ggaccgtcgc ggccatccgc gaattccagt
     3721 cgctgttcgg actgccggtc accgggcagc cggaagaggc cgtctacgcc aagatgcgcg
     3781 agatcggcct gaccaattaa gcctgaccga ttaagctgtg ccggcgtgtc gatttcccgg
     3841 caggaacagt cgatttccgc tggagaaact ccaggagggc cgccatgcgc gtgactgccg
     3901 atctatgggt ttcggccctt gtgcgccggg tgttcagcgc cgggggcttt gccgccgtcg
     3961 tcaagcgcgg cgcgacggaa gccggcgctg tgttcgtcct gtcaagggac cggatggggg
     4021 aggtgacgct ctatggaccg gcgccgcaga ccagctacga ttcggccaag ccggatgacc
     4081 ggcatttcct cattgttgga gcgggcgagg cggctgacgc gctcgatgcg cggctggagc
     4141 gggaaaagcg cttcgaccct gatatctggg tcgtggaaat tgaagccggt tcggttcccg
     4201 tcgaggaact gatttcactc aagacgccct agatttccga tcccggcccg gcgcgggcgt
     4261 atcggcgttg gccggtgtgt ggacgatgcc gtggaggctg ttcgacaggc ttgccgcttt
     4321 ccattcggcc actttttgtc gggcaaggtc atggtggagc gtgcggtagc ggtccaggaa
     4381 gacgcggatt gcctgcggat gcggaaacag cgacgggaac gccgcgacgg taaccaggaa
     4441 cgccttgtcc tcgccgacgt cgagcgcccg cagcgccacc agcagcgacg aatagttcgt
     4501 gctttccgtc agcgatcgcg ccgtagcgaa atcaacaccc agcgcgtcgg ccaatgcggt
     4561 ctggaagaag gcggcattgc ccgtcagcgc cgtctcccgc aatttcacat aggtcgagcc
     4621 gtcgggcgcg ttgatgttgg cggtcccctt atgctcatag ccgacgatgt gttcataatc
     4681 ggccatgacc tcgcgcagcc ggtgccgcgc cgtctcggcg gcgtgcccgg ccggggctgg
     4741 cgcatccgcc ttcgctggag cggcttcgac gggaccggct acattttctg ccggtgtatt
     4801 ggttcgcacc aatgtcggct tttccagcac cctgatgaga tcggcgattg ccggggtcaa
     4861 gcccttgcgc cgcgcgatcg cgcgggcatg gggaaggcca tgccgtccga tcagggcaat
     4921 gaggtcgacg tcaccaagcg catgcgagcg gatcagcagc ggagcggcaa tgtccaccgg
     4981 ttcgtcgcaa agacggcgaa ccagcgctgg tggcggatat tcgcattcgg acagggcggc
     5041 ggcgacgaag cggcgcgatt caaccgaaac gccgtcgaaa agcggcaggg ccagatcgtc
     5101 gatctgcgcg atctcgcgcc gggaagggcg ggtcagcgaa cagaaggcgg aaatggcggc
     5161 gcgaaacagc ctttcggcct tgcccgcctg ccttcggccc ggttcgtccc ggtccgattt
     5221 gcctcggccc gactgtccct ggatgctgat ctgcctgaaa tccgacgatg acacgcgcgg
     5281 tacgccttga tgcactcgat acacgatgcc gtctgcccga ccgttacaag gccggcttca
     5341 ctgggcggca acaacaacaa attagcggcg atccgttagc gttccgttaa ctctctgggg
     5401 gccttattga cgattggagg cgttgcgttg tgctccgggc caaccgagag gatgcacgaa
     5461 tcatgggcag agtactgtct ttcatgccgc gtcaggcggc gagcgttcca ccatcgtcaa
     5521 atgccgggaa gccggccagc gtcatcatct ttccgggcgt gcgctatgag cgtgggccag
     5581 gcgagcggcg gcctgagcaa gcttcgaagg ccgacataca ggtcgcggcg ccggcgcctc
     5641 gcagatagcc attcgccgtt tcagggctgg cgcagggtgc aggaactgtg cccaagaaaa
     5701 cgcatgacgg ctggtttcca ggtttcgtcc ggtacgaagc tgcgcaccac cacaatgcct
     5761 tcggcgatgg gcgcttcgac gaaaaccgat ccttgatatt cgggtggcag atccttgcgc
     5821 acctcgatca tctggccggg cgtcagcacg accgcatcga gccggcctct cggcgcgcca
     5881 tggtcgttga agctatagat gttgtggcca ttgcggtcat acaccgaaac ggaccagaac
     5941 ggcacggccg caggcggtcc tttgagctgg accatgccat cgttgagatt aaagcggcag
     6001 gcagcc
//
LOCUS       DASYCS010000078         4120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4944165,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000078 DASYCS010000000
VERSION     DASYCS010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4944165"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VGM46_02765"
     CDS             complement(<1..288)
                     /locus_tag="VGM46_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013894747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEY4191545.1"
                     /translation="MTSKILSRARAATIALLLLGGSAAAPAYAASTTGHGPESVADLA
                     QGLLGAVVNISTSQTVKGTEGPGAVPMPQLPEGSPFQDFFDDFFKNREGDKG"
     gene            complement(472..1377)
                     /locus_tag="VGM46_02770"
     CDS             complement(472..1377)
                     /locus_tag="VGM46_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEY4191546.1"
                     /translation="MRGRKYLLYDVFTAKRFAGNPLAIVLDSEGLDSEAMQAIAREFN
                     LSETSFVLPPENPRHRARVRIFTPDYEMPFAGHPTVGTAVALAEMGDSSDGIFVLEEN
                     IGPVRCAVAKNDGAAYGEFDLAKLPERLALNCDAEAIGAALGLAPQEVGFENHHVSFW
                     SAGVPYVTVPVADLKAAARVRLDNEAWAKLAPKKSEWAFASPYIYCRETVNHENAFHV
                     RMIVPGTPSYEDPATGSAAAAFAGAIMHFDGPRDGASRFWLEQGLEMGRPSRIRLELD
                     VAQGVLASARIGGHAVKVAEGTLFV"
     gene            1488..2786
                     /locus_tag="VGM46_02775"
     CDS             1488..2786
                     /locus_tag="VGM46_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019858964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY4191547.1"
                     /translation="MDQIVNPPAMETTSAIEAALGLDHGGPGKKRRRGWLYVLAALVL
                     IAAAIVGYRMYANSAVRIDYTTVPAATADLTVQVSATGTLQPLTQVDISSELSGVIRS
                     VAVNENQQVKKGDVLAALDTSRLEAQIERAQASASAAEANVETARITVKENEQALVRT
                     AELAKRGQTTNQALEAATATRDRAKAALDSANANLAISNADLKLQQSDLEKSTIYAPI
                     DGIVLTRSVDPGQTVASSLQAPVLFVIAADLKSMELKAAIDEADIGTVKTGQHARFTV
                     DAFPDRPFDAEIRDISFASLTTDGVVTYDARLEVANDELLLRPGMTATVSVVTKQAKS
                     ILAVPATAFRYKPAPTARREGGWSLTSLFTGRMGARRGGNRQPQAAAPTDGSRTLYIL
                     KNGRPHAVEVKVGATNGDLTEILSGLSEGDQVITASQQRG"
     gene            2791..3498
                     /locus_tag="VGM46_02780"
     CDS             2791..3498
                     /locus_tag="VGM46_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006205001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4191548.1"
                     /translation="MAKPLLITFDKVWKTYGQGEARVNALAGVDLSIRQGEFVAIMGP
                     SGSGKSTSMNIIGCLDTPSAGTYSFMGVDAGRLDRARRATLRNLYVGFVFQGYNLLPR
                     TTAAENVELPLIYRGAAAGERRALAMQALAEVGLTGREHHTPAELSGGQQQRVAIARA
                     IVTRPTLLVADEPTGNLDTARSHEIMELLTRLNTELGLTVVMVTHEADIAAYARRTIR
                     FLDGHVASDMAGLEMAQ"
     gene            3495..>4120
                     /locus_tag="VGM46_02785"
     CDS             3495..>4120
                     /locus_tag="VGM46_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015317318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4191549.1"
                     /translation="MIWETVRLALLSVRRNALRSFLTLLGIVIGVAAVIAMLTIGNGT
                     TQKVKADISKLGSNLLVVRSGRAAGPSANDTIARQLNDGDVAALRKHLTGARAISPAS
                     QKQVRIIFGTENLTSGVTGTDSDYLIARDWKLTAGRAFSDSETRSGTAVCLIGETVRQ
                     QFFGAGDPVGETVRVNRTSCKIVGLLESKGFTGFGQDQDNVVLMPLAA"
BASE COUNT          802 a         1374 c         1218 g          726 t
ORIGIN      
        1 gcccttgtcg ccctcgcggt tcttgaagaa atcatcgaaa aagtcctgga atggcgagcc
       61 ttcgggcagt tgcggcatcg gcaccgcgcc tgggccttcg gttcccttca ccgtctgcga
      121 ggtcgagatg ttgaccaccg cgcccagcaa accctgcgcc aggtcggcaa ccgattccgg
      181 cccatgtccg gttgtcgacg ccgcataggc gggcgccgcg gcgctgccgc caagcagcaa
      241 cagggcgatg gttgcggcac gagcgcgcga aagaattttg gacgtcatcg aggctcccgg
      301 cattcaaaaa agcatgcgct gtgcaaacag cgccacgcgc catgttggca ataaatacgg
      361 cacgtttgcg gccttggcca tgcaggaccg ccaaatccct gtgctttatt cccgctaagg
      421 aaactggttc cgtcgcttgc cataccgcga catagttcga tcaataatcc gtcagacgaa
      481 aagcgtgcct tccgcgactt tcacggcatg gccgccgatc ctcgccgaag ccagcacgcc
      541 ctgcgccaca tcgagttcaa ggcgaatccg cgatgggcgt cccatctcca gcccctgttc
      601 cagccagaag cgcgaggcgc cgtcgcgtgg gccgtcgaaa tgcatgatgg cgccggcaaa
      661 agccgcggcc gctgatccgg tcgccggatc ttcgtaggat ggcgtgcccg gcacgatcat
      721 ccggacatgg aacgcatttt cgtggttcac cgtctcgcgg caatagatat agggactggc
      781 gaaagcccat tcgcttttct tcggagcgag cttcgcccac gcctcattgt ccagccttac
      841 cctggccgcc gccttcagat cggcaacggg aacggtcaca taaggaacgc cagccgacca
      901 gaacgagaca tgatggttct cgaagccgac ctcctgcggc gcaagcccga gcgcggcgcc
      961 aatcgcctcg gcgtcgcaat tgagcgctag gcgctccggc agcttggcca gatcgaactc
     1021 gccataggca gcgccgtcgt ttttggcgac tgcgcagcgc accgggccga tattttcttc
     1081 cagcacgaaa atgccgtccg aactgtcgcc catttcggcc agcgcgaccg ccgtgccaac
     1141 ggttggatgg ccggcaaaag gcatttcata atccggcgtg aagatgcgga cgcgagcgcg
     1201 gtgccgcggg ttttccggcg gcagtacgaa cgaggtttcc gacaagttga attcgcgcgc
     1261 gatggcttgc atcgcttcgc tatcgagacc ttcgctgtcc agcacgatgg ccaacgggtt
     1321 gccggcaaag cgcttggccg tgaaaacatc gtagagcagg tatttacggc cgcgcatcga
     1381 caaactcctc gactcattgc caacatgggc gaaatttaat ttgaaatccg ggccgttagg
     1441 cctgatttgt catcgcatag ggacaacagg caacataggg gtccagcgtg gaccagattg
     1501 tcaatccgcc agcaatggaa acgacttcgg ccatcgaggc ggcccttggt ctcgaccatg
     1561 gcggcccggg taagaaacgc cgccgtggct ggctttacgt gctggcggcg cttgtcctca
     1621 tcgcagccgc catcgtcggc tatcgcatgt atgccaattc ggccgtcagg atcgactaca
     1681 cgaccgtccc ggcggccacc gccgatctta ccgtccaggt ttcggcgaca ggcactcttc
     1741 aaccgctgac acaggtcgat atttcaagcg agctttccgg cgtcatccgc tcggtcgccg
     1801 tcaacgaaaa ccaacaggtc aagaagggcg acgtgctggc ggcgctcgat acctcccgcc
     1861 ttgaggccca gatcgagcgg gcgcaggctt cggcaagcgc ggccgaggcc aatgtcgaga
     1921 ccgcccgcat caccgtgaaa gagaatgaac aggcgcttgt gcgcaccgcc gaactggcca
     1981 agcgcggcca gacgacgaac caggcgctgg aggcggccac cgccacgcgc gaccgcgcca
     2041 aggcagcgct cgacagtgcc aacgcaaatc tcgccatctc caatgccgat ctgaagctac
     2101 aacagtccga tctcgagaag agcaccatct acgcccccat cgacggcatc gtgctgaccc
     2161 gctccgtcga tcccggtcag accgtcgcct cgtcgctgca agcgccggtg ctcttcgtca
     2221 tagccgccga tctgaaaagc atggaattga aggctgccat agacgaggcc gatatcggta
     2281 cggtgaagac cgggcagcac gcgcgtttca ccgtcgatgc tttcccagac cgtcctttcg
     2341 acgccgaaat tcgcgatatt tccttcgcct cgctgaccac ggatggcgtg gtcacctatg
     2401 acgcccggct ggaagtcgcc aatgacgaac ttctgctgcg ccccggcatg acggcaaccg
     2461 tctccgtcgt gaccaaacag gccaaaagca tacttgccgt tcccgccacc gcattccgct
     2521 acaagcccgc gccaaccgcc cgccgtgaag gcggctggag cctgacgagc ctgttcaccg
     2581 ggcggatggg cgcacgccgt ggcggcaacc gccagccgca ggcggctgcg ccgaccgatg
     2641 gctcacgcac gctttacata ttgaagaatg gccggccaca tgcggtggaa gtgaaagttg
     2701 gcgccaccaa tggcgatttg accgaaatcc tctccggcct ttccgagggc gatcaggtca
     2761 tcacggcatc gcagcagcgg ggctgacccg atggcaaagc cgctactcat cacgttcgac
     2821 aaggtctgga agacctatgg ccagggcgaa gcgcgggtca atgcattggc cggcgtcgat
     2881 ctctccatcc gccagggcga attcgtcgcc atcatggggc cttcgggatc gggcaagtcg
     2941 acctcgatga acatcatcgg ctgcctcgat acgccaagcg ccggcaccta ttccttcatg
     3001 ggcgtcgacg ccgggcggct cgaccgcgcc cgccgcgcca ccctgcgcaa tctctatgtc
     3061 ggcttcgtct ttcagggcta caacctgctg ccccgcacca cggcggctga aaatgtcgaa
     3121 ctgccgctga tttatcgcgg agccgccgcc ggcgagcgac gcgcacttgc catgcaggcg
     3181 ctggccgagg tcggcctgac cgggcgtgag caccacacgc cagccgaact ttccggcgga
     3241 cagcaacagc gtgtcgccat tgcgcgcgcc atcgtcacca gaccgacgct tctcgtcgcc
     3301 gacgagccga ccggcaacct cgacaccgcg cgcagccacg aaatcatgga gcttttgacc
     3361 cggctcaaca ccgaactcgg cctgaccgtg gtgatggtca cccatgaggc ggacatcgcc
     3421 gcctatgccc gacgcaccat ccgctttcta gacggccatg tcgcttccga catggccgga
     3481 ttggaaatgg cccaatgatc tgggaaaccg ttcgcctggc acttctgtcc gtcaggcgca
     3541 atgcgctgcg ctccttcctc accttgcttg gcatcgtcat cggcgtcgcc gccgttatcg
     3601 ccatgctgac catcggcaat ggcaccacgc aaaaggtcaa ggcggatatt tccaagctgg
     3661 gttccaacct gcttgtcgtg cggtcgggcc gcgcggcggg gccgagcgcc aacgatacca
     3721 ttgcgcgcca gctcaacgac ggcgacgtgg cggcgcttcg caagcacctg accggcgctc
     3781 gcgccatatc gccggcttcc cagaagcagg tccgcatcat tttcggcacg gaaaacctca
     3841 ccagcggcgt caccggcact gatagcgact atctgattgc gcgcgactgg aaattgaccg
     3901 ccgggcgggc cttctcggat tcggaaacgc gcagcggcac cgcagtctgc ctgatcggcg
     3961 agacggtgcg ccagcaattc ttcggcgcgg gcgacccggt tggcgagacg gtgcgcgtca
     4021 accgcacttc atgcaagatc gtcggcctgc tcgaatcgaa gggcttcacc ggctttgggc
     4081 aggaccagga caatgtggtg ctgatgccgc ttgccgccta
//
LOCUS       DASYCS010000079         2733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2190083,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000079 DASYCS010000000
VERSION     DASYCS010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2190083"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /gene="cobA"
                     /locus_tag="VGM46_02790"
     CDS             <1..601
                     /gene="cobA"
                     /locus_tag="VGM46_02790"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEY4191550.1"
                     /translation="TQAQINTLLVELGREGKRVVRLKCGDPLVFGRAGEEMAALREAN
                     ISYEVVPGVTAAFAAAADFELPLTLRGVTSSMVFTTGHDLKGKSLPDWAKLAISGATV
                     AVYMGRSVAAEVAGRLVASGLSPDTAVAVVENASLVNRRRFHGTLADLPSLQGRVDLD
                     GPVMTIIGDAVAGANFEGSQPLAARRSARTDRAMEGMQA"
     gene            598..897
                     /locus_tag="VGM46_02795"
     CDS             598..897
                     /locus_tag="VGM46_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2849 domain-containing protein"
                     /protein_id="HEY4191551.1"
                     /translation="MKVLTANRLIDGEAVWYADGVWVEAIDAAEIAGDKAGEERLDEA
                     GKAASAANLVVDVNLIDVEMLDGSIHPTRLRERIRAKGPTIRNDLGKQARFQRAA"
     gene            962..2632
                     /locus_tag="VGM46_02800"
     CDS             962..2632
                     /locus_tag="VGM46_02800"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020012471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HEY4191552.1"
                     /translation="MYRYDEFDHAFVKARVAQFTDQVERRLAGELTEDEFKPLRLMNG
                     VYLQLHAYMLRIAVPYGTLHARQLRMLAHIARKYDKGYGHFTTRQNIQFHWPALSDVP
                     AILADLASVEMHAIQTSGNCIRNVTADHFAGAAADEVADPRPYAEILRQWSSVHPEFS
                     YLPRKFKIAVTGAERDRAAIKTHDIGLHLKKNEKGELGFAVYIGGGLGRTPMVAKKIR
                     DFLPQEHLLSYTTAILRVYNLHGRRDNKFKARIKILVHETGTEELASWIDAEWQALKD
                     GELKLPEQDVAAIGAYFAPPPLMERPEGDDKVRLDRLDSRSFSEWLGQNVTTHRNPDY
                     AAVTVSLKGIGETPGDISDSQMEAIADLAERYGFDEVRVTHEQNLVLPHVARADLKAV
                     YDGLVETGLATANLGLISDIIACPGLDYCALANARSIPVAQQISQRFSSLERQREIGE
                     LKLKISGCINACGHHHVGHIGILGVEKKGAELYQITLGGSADENTTVGEIIGRGFGPD
                     EIVDAVEAIVETYLSIRNDQSERFLDVYRRVGPAPFKEVLYGAEAKAA"
BASE COUNT          515 a          836 c          908 g          474 t
ORIGIN      
        1 gacgcaagcg cagatcaaca cgctgctggt cgaactgggc cgtgaaggca agcgcgtggt
       61 gcggctcaaa tgcggcgatc cgctggtgtt cggccgtgcg ggcgaggaga tggcggcgct
      121 gcgcgaggcg aatatttcct acgaggtggt gcccggcgta actgccgcct ttgccgcagc
      181 cgccgatttt gaactgccgc tgacattgcg cggcgtgacc tcgtcgatgg tgttcaccac
      241 cgggcatgac ctgaaaggca agtcgctgcc ggattgggcg aagcttgcca tctccggggc
      301 gacggtcgcc gtctatatgg ggcgctcggt cgcggcggaa gtcgcgggcc ggctggtcgc
      361 gtcgggcctt tctcccgaca cggcggtggc ggtggtggaa aacgccagcc ttgtcaaccg
      421 ccgccgcttc cacggcacgc tggccgacct gccttcgttg caggggaggg tcgacctgga
      481 cgggccggtc atgaccatta tcggcgatgc cgtcgccggg gcgaatttcg aaggctcgca
      541 gccgttggcg gcgcggcgca gcgcgcgcac ggatcgcgcc atggaaggaa tgcaggcatg
      601 aaagttctga ccgctaaccg gctgatcgat ggcgaagccg tatggtacgc cgacggcgtc
      661 tgggtcgagg ccatcgacgc cgccgaaatt gccggcgaca aggccggtga agaacgcctg
      721 gatgaggcgg gcaaggccgc gtcggcggct aatctggtgg tcgatgtcaa cctgatcgat
      781 gtcgagatgc tcgacggttc cattcacccg acgcggctgc gcgaacgcat ccgcgccaag
      841 ggcccgacca ttcgcaacga tctcggcaag caggcccgct tccagcgggc ggcgtaggaa
      901 cagaaacagg cccattgcgc gactgaagcc tcaagggaag accccatctt aagggaccat
      961 catgtaccgc tacgacgaat tcgatcatgc ctttgtgaag gcccgtgtcg cccagttcac
     1021 ggaccaggtg gagcggcggc ttgccggcga gttgaccgag gacgagttca agccgctgcg
     1081 gctgatgaac ggcgtctacc tccagcttca cgcctatatg ctgcgcattg ccgttcccta
     1141 tggcacgctg catgcgcggc agttgcggat gctggcgcat atcgcgcgca aatatgacaa
     1201 gggctacggc catttcacta cgcgccagaa catccagttc cattggccgg cgctgtcgga
     1261 cgtgccggcg atcctggccg acctggcgtc ggtggagatg catgccatcc agaccagcgg
     1321 caattgcatc cgcaacgtga cggccgatca tttcgccggc gccgccgccg acgaagtggc
     1381 agacccgcgc ccttacgccg aaatcctgcg ccaatggtcg tcggttcatc cggaattttc
     1441 ctacctgccg cgcaagttca agatcgcggt gaccggggcg gagcgcgacc gcgccgccat
     1501 caagacgcat gatatcggcc tgcacctgaa gaagaacgag aagggcgagc ttggctttgc
     1561 cgtctatatt ggcggcggac ttggccgcac gccgatggtc gccaagaaaa tccgcgattt
     1621 cctgccgcag gagcacctgc tttcctacac gacggcgatc ctgcgcgttt acaacctgca
     1681 cggccgtcgc gacaacaagt tcaaggcgcg gatcaagatc ctcgtccacg agaccggaac
     1741 ggaagagctg gcaagctgga tcgatgccga gtggcaggcg ttgaaggacg gcgagctcaa
     1801 gctgccggaa caggacgttg ccgccattgg ggcctatttc gcgccgccgc cactgatgga
     1861 gcgcccggag ggcgacgaca aggttcggct ggaccggctg gattcgcgtt cgttcagcga
     1921 atggctgggg cagaacgtta cgacgcaccg caatcccgac tatgcggcgg tgacggtctc
     1981 gctcaagggc atcggcgaaa cgccgggcga catcagcgac agccagatgg aggcgatcgc
     2041 agaccttgcc gaacgctacg gcttcgatga agtgcgggtg acgcatgagc agaacctcgt
     2101 tttgccgcat gtggcgcgcg ccgatctcaa ggccgtctat gacgggctgg tggaaacagg
     2161 cctggcgacc gccaatctgg gcttgatcag cgatatcatc gcctgccccg gcctcgacta
     2221 ttgcgcgctg gccaatgcgc gctccattcc ggttgcccag cagatttcac agcgcttttc
     2281 ctcgctggag cgccagcggg aaataggcga gctgaagctg aagatttcag gctgcatcaa
     2341 tgcctgcggg caccatcatg tcggccatat cggcattctc ggcgtcgaga aaaagggcgc
     2401 cgagctgtac cagatcacgc ttggcggatc ggcggacgag aacaccaccg ttggcgaaat
     2461 catcgggcgc ggctttggtc ccgatgagat cgtcgatgcc gtcgaggcca ttgtcgaaac
     2521 ctacctgtcc atccgcaatg accaatcgga acgtttcctc gacgtctatc gccgggtcgg
     2581 gccggcgccg ttcaaggagg tgctctatgg agcagaagcc aaggctgctt gagcaggcag
     2641 ccggcgcgga agcaggcgtt gccgccgacg cggcggggct ggacgcgctt tatggccacc
     2701 tgccggcgct ggagatcatc gagcagtcgg cta
//
LOCUS       DASYCS010000080        12653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2915206,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000080 DASYCS010000000
VERSION     DASYCS010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 12653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12653
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2915206"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="VGM46_02805"
     CDS             complement(<1..722)
                     /locus_tag="VGM46_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014764067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY4191553.1"
                     /translation="MSDLTRLTISQARAKLQAREITASEITDAYLAAIEQANPSLNAY
                     VTVTGDKARAMAKASDARLAKGEGGALEGIPLGVKDLFGTEGVHTQAASHVLDGFKPR
                     YESTVTANLWADGAVMLGKLNMDEFAMGSSNETSYYGAVVNPWRATGSNRQLVPGGSS
                     GGSASAVSGFLCAGATATDTGGSIRQPAAFTGTVGIKPTYGRCSRWGIVAFASSLDQA
                     GPIARDVRDAAILLKSMASVDA"
     gene            complement(722..1189)
                     /locus_tag="VGM46_02810"
     CDS             complement(722..1189)
                     /locus_tag="VGM46_02810"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY4191554.1"
                     /translation="MAVEIAIETPLQDDVREMVAALDAHMRPLTPREFQFQLTVEQMA
                     DPSTTLFVARDEAGQAVGMGALKEHGDGLGEVKRMFTRPQVRGQRVGAQLLQNIERLA
                     RQKGLARVVLETGEAPGFEPAYRVYEHGGFQVCGAVLDYPDSGHSRFYEKSLA"
     gene            complement(1196..1483)
                     /gene="gatC"
                     /locus_tag="VGM46_02815"
     CDS             complement(1196..1483)
                     /gene="gatC"
                     /locus_tag="VGM46_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEY4191555.1"
                     /translation="MSVDLVTVKRVARLARIAVSEEDAKRMAGELNAILGFVEQLNEV
                     DVTGVEPMTSVTPMAMKKRQDVVTDGGKAADIVANAPATEESFFLVPKVVE"
     gene            complement(1553..2260)
                     /locus_tag="VGM46_02820"
     CDS             complement(1553..2260)
                     /locus_tag="VGM46_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEY4191556.1"
                     /translation="MKLTWYGHSAFAIETADAKILIDPYLIGNPSWDGGWEKPAEGVT
                     HVLLTHGHDDHISGAAEVLKKSGAQLVANFELCMYLVGKGVSGDRINPGNIGGTVDCG
                     SFTTTFVQALHSSSSQGKDGQNTYLGNPGGLVLHFTNDKTLYHMGDTDIFSDMALIHE
                     LHQPQIGIVPVGDRFTMGGAVAALACQRYFKFETVVPCHFGTFPMIDQTADKFTAGME
                     GSGIEIARPKIGATIEV"
     gene            2330..2824
                     /gene="ruvX"
                     /locus_tag="VGM46_02825"
     CDS             2330..2824
                     /gene="ruvX"
                     /locus_tag="VGM46_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015317754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEY4191557.1"
                     /translation="MGTLTIEELPARLADGKTLAGLDLGDKTIGVAVSDRNFSFAHPR
                     PVIMRKKFTLDATALLSLLQKENVGAIVIGLPVNMDGSEGPSAQRSRAFVRNMGPLSD
                     LPFVLWDERLSTVAVERTLIEMDFSRKKRAGKIDSAAAAFILQGALDRLRALNGPAPS
                     SDRA"
     gene            complement(2754..3089)
                     /locus_tag="VGM46_02830"
     CDS             complement(2754..3089)
                     /locus_tag="VGM46_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6105 family protein"
                     /protein_id="HEY4191558.1"
                     /translation="MRYLFAAWVAPLVLFWGWYFLSLNDMNFGYVMLSRQLHDLVFEL
                     YGQMLGIDPKIIPGMVAKACFFDSLLVMAIWAFRRRRTIAGWIRSMRDRWMGQGRSRH
                     GAGRGLPAK"
     gene            complement(3393..5024)
                     /locus_tag="VGM46_02835"
     CDS             complement(3393..5024)
                     /locus_tag="VGM46_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019858235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation response protein"
                     /protein_id="HEY4191559.1"
                     /translation="MSDDVTNQPPPLTGGNAWRGDPLLIQLAEQFSEPVRKDLDALGR
                     FVLTHEAQDLARLANTETPKLKTHDRQGRRLDQVEFHPAYHALMRRSVANGLHSSIWE
                     NGDAEIGRRHQVRAARFYLTAELEAGHLCPLTMTSASLAALMASPKLFREWAPRVTTR
                     KYDQSQKPPVEKTGLTLGMGMTEKQGGTDVRANTTRAERAGSTGFYRLTGHKWFMSAP
                     MSDAFLVLGQAAEGLSCFMVPRILGDGTGNGFRFQRLKDKLGNRSNASSEVEFDNAIG
                     EMVGEPGAGVKTIMDMVTLTRLDCAVASSALMRAGLAEAVHHTRHRQVFGAKLVDQPL
                     MQRVLADMALDVAAATALSFRLARSFDEAAGDRGEAAFARAMTPVVKYWVCKIAPSLL
                     YEAMECMGGNGYVEETPLARYYREAPVNAIWEGSGNVMALDVLRVLTRAPGLFEEVLA
                     GIDRDLGAGGRGTIGVLRAAMQVAETDEGSARLLTEQLALSAAAAELRRLGAGRIADA
                     FVETRLAGQWRNTYGMIDARHDARMIVDTLYPPTN"
     gene            5199..6161
                     /locus_tag="VGM46_02840"
     CDS             5199..6161
                     /locus_tag="VGM46_02840"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEY4191560.1"
                     /translation="MTDAPSLPLYPHRHLLGISTLSPTDIELLLDRADRAVAISRQPE
                     KKTSTLRGRTQINLFYEASTRTQSSFELAGKRLGADVMNMAVASSSVKKGETLIDTAM
                     TLNAMRPDILIIRHQSAGAAALLAQKVGCSVVNAGDGAHEHPTQALLDALTIRRAKGP
                     LSKLIVAICGDILHSRVARSNIMLLNALGAQVRVVAPSTLLPSGIDRMGVIVAKSMAE
                     GLKDADVVMMLRLQRERMAGAFVPSVREYFHYFGLDAEKLKAAKPDALVMHPGPMNRG
                     VEIASEIADGPQSVIQEQVEMGVAVRMAVMEALLDPRRNEGRGA"
     gene            6158..7444
                     /locus_tag="VGM46_02845"
     CDS             6158..7444
                     /locus_tag="VGM46_02845"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019858237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEY4191561.1"
                     /translation="MSITVFQRARIVDPSRGIDEVGSVVVDGKKILAAGKSALNQGAP
                     EGATVVDCVGKAILPGLIDSRVFIGEPGGEHRETIASASHAAAAGGVTSIVMMPDTDP
                     VIDNVALVEFVLRTAKDTASINVFPAAAITKGLEGREMTEFGLLREAGSVAFTDGRHT
                     IASSLVMRRALTYARDFGAMIAHETQDGDLASQGVMNEGLYASWLGLSGIPREAETIP
                     LERDLSLARLTGGAYHAAKISTAMAAGAIARAKADGANVSAGVSIHNLSLNENDVGEY
                     RTFFRLTPPLRAEDDRLAMIEAVKDGTIDVIVSSHDPQDVDTKRLPFADAAAGAIGLE
                     TLLGAGLRLYHNGDVSLLRLVEVLSTAPARLYGLPGGTLKPGAPADFVLVDLDEPWVV
                     SESIIRSRSKNTCFEGARLQGKVLQTMVAGSTVFSA"
     gene            7473..8060
                     /gene="plsY"
                     /locus_tag="VGM46_02850"
     CDS             7473..8060
                     /gene="plsY"
                     /locus_tag="VGM46_02850"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEY4191562.1"
                     /translation="MTITSIIALIFGYLLGSIPFGLLLTRAAGLGDVRSIGSGNIGAT
                     NVLRTGHKGLAAATLILDALKGTVAVLVAAHFGPELGLWAGFGAFIGHLFPVWLGFKG
                     GKGVATYLGVLIGLAWQGALVFAAVWLVMAAVLRYSSLAALAAAVIVPIVLWFQASPQ
                     IAGLFALMSVIVFIKHRANIARLMAGTEGRIGKKG"
     gene            8057..9178
                     /gene="dprA"
                     /locus_tag="VGM46_02855"
     CDS             8057..9178
                     /gene="dprA"
                     /locus_tag="VGM46_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEY4191563.1"
                     /translation="MKERAVAPHLSDRQRLAWLRLIRTQNVGPASFRDLINRFGSAEA
                     ALEMLPELMISGGLNRLVRIPTVAEAEAEMEAAGKAGARFVGIGEPGYPPLLRNIDHP
                     PPLLALKGDGAVFDLPAIAIVGARNASLAGIKMARTLAAELGRQGYAIVSGLARGIDT
                     AAHQGSLATGTVGVLAGGLDMPYPPENTGLLHDICEHGGAVVSEMPFGWQPRAQDFPR
                     RNRLVAGAAFGLVVVEAAKRSGSLISARLAGEMGRLVFSVPGSPLDPRAGGTNGLLKD
                     GAILVTEAADVLEAIAPLTGAPSRTSLEEPPDFSATPPPGEGDRDLVIEALGPTPVSP
                     DEIIRHTRLHPAQVFMVLLELDLAGRLERHAGGSVSLVF"
     gene            complement(9198..9272)
                     /locus_tag="VGM46_02860"
     tRNA            complement(9198..9272)
                     /locus_tag="VGM46_02860"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9238..9240),aa:Lys,seq:ttt)
     gene            9659..10159
                     /locus_tag="VGM46_02865"
     CDS             9659..10159
                     /locus_tag="VGM46_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BA14K family protein"
                     /protein_id="HEY4191564.1"
                     /translation="MNKIVSGLLATVLSISFVGAEMLPVNAASIYVPQTSTAISSEIV
                     PVVDRGGGNFNRGRVFRHGSRNSWSGNRSWNGNRNYRGGGRGYWNGHRGSSYYRRGYR
                     RHGNLWFPLAAFATGAIISGAIANDRPPAYSGNSHVQYCYNRYKSYRASDNTFQPYNG
                     PRQQCR"
     gene            complement(10222..11715)
                     /gene="glpK"
                     /locus_tag="VGM46_02870"
     CDS             complement(10222..11715)
                     /gene="glpK"
                     /locus_tag="VGM46_02870"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEY4191565.1"
                     /translation="MTGFVLAIDQGTTSTRAILFDGHMRVAGIGQKEFAQFYPASGWV
                     EHDPEELWSSVVWSVKAALKKAGRQASEVAAIGITNQRETIVVWDRETGKAIHPAIVW
                     QDRRTANLCQKLKKQGLEKKFTKKTGLLLDPYFSATKIAWLLGKVKGARKRAERGELL
                     AGTIDSFLIWRLTGGAMHATDATNASRTLVYNIEKNEWDKELLGILDIPARMLPQVKD
                     CADDFGVTDKSLFGSGIRILGVAGDQQAAAIGQACFKPGMMKSTYGTGCFALLNTGTD
                     IVRSKNRLLTTIACRLEGRTTYALEGSIFIAGAAVQWLRDGLKIIDRAGEAGLLAAKA
                     DPAQDVYLVPAFVGLGAPHWDADARGAIFGLTRNSGRPELARAALESVAYQSGDLLDA
                     MRRDWKGETTGTVLRVDGGMVASDWTMQRLADILDAPVDRPTILETTALGAAWLAGSR
                     AGMWPQAEEFAESWALDRRFEPKMDAGQRTAKLKGWRDAVRRTASAR"
     gene            complement(11736..>12653)
                     /gene="glpD"
                     /locus_tag="VGM46_02875"
     CDS             complement(11736..>12653)
                     /gene="glpD"
                     /locus_tag="VGM46_02875"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HEY4191566.1"
                     /translation="GGTEEVKARLLVNAAGPWVDHVLSGVVGRQDVHNVRLVQGSHIV
                     VRRKFEDPRAYFFQNKDGRIIFAIPYEEDFTLIGTTDRDFDGDPHAVKISDAEIDYLC
                     AAASEYFAEPVRRADIVWTYSAVRPLYDDGASKAQEATRDYVLKGEGGDGRAPIVDAF
                     GGKITTYRRLSESMLEKIEGFLGKRGKAWTANAPLPGGDFPATGFDAEVRGLKAAYPF
                     LDQRLARRLTRLYGTRARLLLGEARSQADLGRDFGGDLHEAEVRYLAGHEWAMTAEDV
                     LWRRTKRGLHFDAGQVAALEDFMRTLNMR"
BASE COUNT         2258 a         4103 c         3932 g         2360 t
ORIGIN      
        1 ttggcgtcca cggaggccat ggatttcaaa aggatcgcgg cgtcgcgcac gtcgcgcgcg
       61 atcgggccgg cctgatcgag cgacgaggcg aaggcaacga tgccccagcg cgagcagcgg
      121 ccataggtcg gcttgatgcc gaccgtgccg gtgaacgccg ccggctggcg gatcgagccg
      181 ccggtgtcgg tggcggtcgc gcccgcgcac aggaagccgg agaccgccga ggccgagccg
      241 ccggacgagc cgcccggcac cagttgcctg ttcgaacccg tggcgcgcca cgggttgacg
      301 acagcgccat aataggaggt ctcgttggac gaacccatgg cgaactcgtc catgttgagc
      361 ttgcccagca tgaccgcgcc gtctgcccac agattggcgg tgacggtcga ttcatagcgt
      421 ggcttgaagc cgtcgagcac atggcttgcc gcctgcgtat gcacgccttc ggtgccgaac
      481 agatccttga cgccgagcgg gatgccttcc agcgcgccgc cttcgccctt ggccagccgg
      541 gcgtcggagg ccttggccat ggcgcgcgcc ttgtcgccgg tcaccgtgac ataggcgttg
      601 agcgaaggat tggcctgctc gatcgccgcc agataggcat cggtgatctc gctcgcggtg
      661 atctctctcg cctgtagctt ggcgcgggcc tgcgaaatcg tcagtcgggt taggtcgctc
      721 atcaggcaag gctcttttcg tagaaacggg aatggccgga atcgggataa tcgagcaccg
      781 cgccacaaac ttggaagcca ccgtgttcat agacgcggta ggctggttcg aaaccggggg
      841 cctcgccggt ttccagcacg acgcgagcca gccctttttg ccgggcaagc cgctcgatgt
      901 tttgcaacag ttgtgcgccg acgcgctggc cacgtacttg cggccgggtg aacatgcgct
      961 tgacctcgcc aaggccatca ccatgttcct tgagcgcgcc catgcccacc gcttggccag
     1021 cctcgtcgcg cgcgacgaac aaggtcgtcg acggatcggc catctgctcg acggttagct
     1081 ggaactggaa ttcgcgtggc gtcagcgggc gcatatgagc gtccagcgca gcgaccattt
     1141 cgcgcacatc gtcctgaagc ggcgtctcaa tggcgatctc gacggccatc cagcgctatt
     1201 ccaccacctt cggcacgagg aagaagcttt cttccgtcgc cggcgcgttg gcgacgatat
     1261 cggcggcctt gccgccatcg gtgacgacat cctgccgctt cttcatcgcc atcggcgtga
     1321 ccgatgtcat cggctccacg cccgtgacat cgacttcgtt cagctgctcg acaaagccga
     1381 ggatcgcatt gagttcgcca gccatgcgct ttgcgtcctc ctcgctcacc gcaatgcggg
     1441 caaggcgcgc gacgcgcttt acagtcacaa gatcaacgga catttgttca gccttttaaa
     1501 ccagcccgcg ctatagccgc gccgcgccgc gcgcgcaaca tgcccggtgt tgctagacct
     1561 ctatcgttgc gccaatcttc gggcgggcga tctcgatgcc cgaaccttcc atccccgccg
     1621 tgaacttgtc ggcggtctgg tcgatcatcg ggaaggtgcc gaaatggcaa ggcaccaccg
     1681 tctcgaactt gaaataacgc tggcaggcca gcgctgccac cgcgccgccc atggtgaagc
     1741 ggtcgcctac cggcacgatg ccgatttgcg gctgatgcag ttcgtggatc agcgccatgt
     1801 cggagaagat atcggtgtcg cccatatggt agagcgtctt gtcgttggtg aagtgcagca
     1861 ccagcccgcc gggattgccg agataggtgt tctggccgtc cttgccctgc gaggaagacg
     1921 agtgcagcgc ctgcacgaag gtggtggtga acgagccgca atcgaccgtg ccgccaatat
     1981 tgccgggatt gatcctgtcg ccgctgacgc ccttgccgac caggtacatg caaagctcga
     2041 aattggcgac gagctgcgcg ccgcttttct tcagcacctc ggcggcgccg ctgatatggt
     2101 cgtcatggcc atgcgtcagc aagacgtggg tgacgccttc agccggcttt tcccagccgc
     2161 catcccatga gggattgccg ataaggtatg gatcgatcag gattttggcg tccgccgtct
     2221 ctatcgcaaa ggcggaatgg ccgtaccagg tcagtttcat gcaggcgtct cctcgatttt
     2281 aggttcgccc aaggatagaa cgctgacgac ttgagtcaaa ggaaagcctg tgggcacact
     2341 tactattgaa gagctacccg cgcggctggc cgacggcaag acgctggccg gactggatct
     2401 cggcgacaag acaatcggcg tggccgtttc ggacaggaat ttttcgttcg cccatccgcg
     2461 cccggtgatc atgcgcaaga agttcacgct ggacgccacg gcgctgcttt cgcttctcca
     2521 gaaagaaaat gtcggcgcga tcgtcatcgg cctgccggtc aacatggacg gttccgaagg
     2581 gccaagcgcc cagcgctcgc gcgccttcgt gcgcaatatg ggaccgctgt cggacctgcc
     2641 attcgtcttg tgggacgagc ggctatcgac cgtcgcggtc gagcggacgc tgatcgaaat
     2701 ggatttttcg cgcaagaagc gcgccggcaa gatcgattcg gcggcggccg cctttatttt
     2761 gcagggagcc ctcgaccggc tccgtgcctt gaacggccct gccccatcca gcgatcgcgc
     2821 atagacctga tccagccggc gatggtacgg cgacggcgaa atgcccagat cgccatcacc
     2881 aaaagcgaat cgaaaaaaca ggccttggcg accatgccgg gaatgatttt cggatcgatg
     2941 cccagcatct gtccgtaaag ctcaaaaacc aggtcgtgca actggcggct cagcatgacg
     3001 tagccgaagt tcatgtcgtt cagcgacagg aaataccagc cccagaacag gaccagcggc
     3061 gctacccagg cggcgaacag atagcgcatc agaatggcct tcccgtatcc gtcgcgacat
     3121 tgccggaaag gcgcgccggc atttgtgctg tcaggaacgg aatcaacagg ggccgcatgg
     3181 cgtttccaat ttggtaacca taactgtgag ccttgcggag gaacgccgca atggaaacca
     3241 tttgttaagg ttaacggaaa ttcacaagtg ctggaatgca ctgttggggc atgagcagtt
     3301 ccgattttta ctgaatcgac agaaccgctt tatctcttgg ttttggccgc atttccggac
     3361 gcaaaaccgt ttcacccttt tgctggaaat gctcagttcg tcggcggata gagcgtatcg
     3421 acgatcattc gcgcgtcgtg gcgggcgtcg atcatgccat aggtgttgcg ccattggccg
     3481 gcgagccgcg tctcgacaaa ggcgtcggcg atgcgccccg cacccagccg gcgcaattcg
     3541 gcggcagccg ccgaaagcgc cagttgctcg gtcagcagcc gtgccgaccc ctcgtcggtt
     3601 tcagccacct gcatggccgc cctcagcacg ccgatggtgc cgcgtccgcc ggcgccgagg
     3661 tcgcggtcga tgccggccag aacttcctcg aacaggcccg gcgcgcgggt aagcacgcgc
     3721 agcacgtcga gcgccatgac gttgccggag ccttcccaga tcgcgttgac cggcgcttcg
     3781 cggtaatagc gcgccaacgg cgtttcctcg acatagccgt tgccgcccat acattccatc
     3841 gcctcgtaga gcagcgacgg cgcgatcttg cagacccagt atttgaccac cggcgtcatg
     3901 gcgcgggcaa aagccgcctc gccgcggtcg cccgccgctt catcgaacga gcgcgccagc
     3961 cggaacgaaa gcgcggtggc ggcggcgaca tcgagcgcca tgtcggccaa aacgcgctgc
     4021 atcagcggct ggtcgaccag ctttgcgccg aacacctgcc gatggcgcgt atggtggacg
     4081 gcctccgcca gcccggcgcg catcagcgcg gaggaggcga ccgcgcaatc cagccgcgtc
     4141 agcgtcacca tgtccatgat ggtcttcacg ccggcgcccg gctcgccaac catctcgccg
     4201 atggcgttgt cgaactcgac ttccgacgag gcgttcgagc ggttgcccaa cttgtccttc
     4261 agccgctgga aacggaagcc gttgccggtg ccgtcgccca atatgcgcgg caccatgaag
     4321 cacgacaggc cctccgccgc ctgtcccagc accaggaagg catccgacat cggcgccgac
     4381 atgaaccatt tatggccggt gaggcgatag aatccggtac tgcccgcgcg ctcggcgcgg
     4441 gtggtgttgg cgcgcacatc ggtgccgccc tgcttttcgg tcatgcccat gccgagcgtc
     4501 agcccggtct tttccaccgg cggcttctgg ctctggtcat atttgcgggt ggtgacgcgc
     4561 ggcgcccatt cgcggaacag cttcgggctt gccatcaacg ccgccagcga ggcgctggtc
     4621 atggtgagcg ggcacagatg cccggcttcc agttcggcgg tgaggtaaaa gcgcgcggcg
     4681 cgcacctggt ggcggcggcc tatctcggca tcgccatttt cccagatgga agaatgcagc
     4741 ccgttggcga ccgagcgccg catcaaggca tgataggccg ggtgaaattc gacctggtcg
     4801 agccgccgtc cctgccggtc gtgcgtcttc agcttcggcg tttcggtgtt ggcgaggcgg
     4861 gcgaggtcct gcgcctcatg cgtcagcacg aaacggccca gtgcatcgag gtccttgcgc
     4921 accggctcgg aaaactgctc ggcaagctgg atcagcagcg ggtcgccgcg ccatgcattg
     4981 ccacccgtca gcggcggggg ctggttcgtc acgtcgtcgc tcacgccctg ttcccatgct
     5041 cgaaggctgc ggccaagaat ccgcgcattg gcgtcgttat agcctaaggc cggcgaggcg
     5101 cgcgacgaaa atgacgggga caaggccgct aatccaaagc ctgcgcttgc taggcttaac
     5161 gccgcgccct atagcaccgc cctgagatga ccgatcagat gacagacgcc ccttccctgc
     5221 cgctttatcc tcatcgccat cttcttggca tcagcaccct ttctcccacc gatatagaac
     5281 tgctgctcga ccgtgcggac cgcgcggtgg cgatctcgcg ccagccggag aagaaaacat
     5341 ccaccttgcg cgggcgcacg cagataaacc tgttctacga ggcatcgacc cgtacgcagt
     5401 cttcgttcga actggccggc aaaaggctcg gcgccgatgt gatgaacatg gcggtggcga
     5461 gttcttcggt gaagaagggc gaaacgctga tcgacacggc gatgacgctg aacgcgatgc
     5521 ggccggacat cctgatcatc cgccatcaat cggcaggcgc cgccgccctt ctggcgcaga
     5581 aagtcggttg ctcggtggtc aatgccggcg acggcgcgca tgagcacccg acacaggcgc
     5641 tgctcgacgc cctgaccatt cgccgcgcca aggggccgct ttcgaaactg atcgtggcca
     5701 tctgcggcga catcctgcat tcgcgcgtgg cgcgctccaa catcatgctg ctcaacgcgc
     5761 tgggcgcgca ggtgcgggtg gtcgcgccct cgacgctgtt gccttccggc attgatcgca
     5821 tgggcgtcat cgtcgccaaa tccatggccg agggcttgaa ggacgccgac gtggtgatga
     5881 tgctgcgcct gcaacgcgag cgcatggcag gcgcattcgt gccttcggtg cgcgaatatt
     5941 tccattattt cgggcttgat gccgaaaagc tgaaggctgc caagcccgac gcgctggtga
     6001 tgcaccccgg cccgatgaac cgaggcgtcg agatcgcctc cgagatcgcg gacgggccgc
     6061 aaagcgttat ccaggaacag gtggaaatgg gcgtcgccgt gcgcatggcg gtgatggagg
     6121 cgctgctcga cccgcgccgc aacgaagggc gcggcgcatg agcatcaccg ttttccagcg
     6181 cgcccgtatc gtcgatcctt cgcgcggcat cgacgaggtc ggctcggtcg tcgtcgacgg
     6241 taagaagatc ctagcggccg gcaaatccgc acttaaccag ggcgcacccg aaggcgccac
     6301 ggtcgtcgac tgcgtcggca aggctatcct gcccggcctg atcgattccc gcgtcttcat
     6361 tggcgaaccg ggcggcgagc accgcgaaac catcgcctcg gccagccatg ccgctgcggc
     6421 cggcggcgtc acctccatcg tcatgatgcc cgataccgat ccggtcatcg acaatgtcgc
     6481 gctggtcgag ttcgtgctgc gcacggccaa ggatacggca agcatcaacg tgttccccgc
     6541 cgccgccatc accaaggggc tggaggggcg cgagatgacc gagttcggcc tgttgcgcga
     6601 ggcgggcagc gtcgccttca ccgatggccg ccacaccatc gccagttcgc tggtcatgcg
     6661 ccgcgcgctg acctatgcgc gcgatttcgg cgcgatgatc gcgcatgaaa cgcaggatgg
     6721 cgacctcgcc tcgcagggcg tgatgaacga agggctttat gcaagctggc ttggcctttc
     6781 gggcattccg cgcgaggccg aaaccattcc gctggagcgc gacctttcct tggcccggct
     6841 aaccggcggg gcctatcacg ccgccaagat ttccaccgcg atggcggcag gcgccattgc
     6901 gcgcgccaag gcggacggcg ccaatgtcag cgccggtgtt tcgatccaca atctgtccct
     6961 caacgaaaac gatgtcggcg aataccgcac gtttttcagg ctgacgccgc cgctgcgggc
     7021 cgaggacgac cggctggcga tgatcgaggc cgtcaaggac ggcaccattg acgtcatcgt
     7081 ttcctcgcat gacccgcagg acgtggacac caagcgcctg cccttcgccg acgcggcggc
     7141 cggcgcgata gggctggaga cgctgctggg ggccggcttg cggctttacc acaatggcga
     7201 tgtttcgctg ttgcggctgg tggaagttct ctccaccgcc ccggccaggc tttacggcct
     7261 gccgggcggc accttgaaac ccggcgcccc ggccgacttc gttctggtcg acctcgacga
     7321 gccatgggta gtcagcgaaa gcatcatccg gtcgcgttcg aaaaacacct gcttcgaggg
     7381 tgcgcggctg caaggcaagg tcttgcaaac catggttgca ggcagcacag tgttttcggc
     7441 ttaggctggc atccagccca tcggggagag ccatgaccat cacttccatc atcgccttga
     7501 tattcggcta tctacttggc tccatcccct tcggccttct gttgacccgc gccgccgggc
     7561 tgggcgacgt gcgctcgata ggctccggca acatcggcgc gacgaacgtg ctgcgcaccg
     7621 gccataaggg gctggcggcc gcaacattga tactcgatgc gctgaaaggc accgtcgcgg
     7681 tgctggtcgc cgctcatttc gggccggagc ttggcctgtg ggcgggcttc ggcgcgttta
     7741 tcggccatct ctttccggtc tggctgggct tcaagggcgg caagggcgtg gccacctatc
     7801 tcggcgtttt gatcggcctt gcctggcagg gcgcgctggt gttcgcggcg gtgtggctcg
     7861 tgatggctgc cgtccttcgc tattcctcgc ttgcggctct ggccgccgcc gtcatcgtgc
     7921 cgatcgtgct gtggttccag gcatcgccgc aaattgccgg cttgtttgcg ctgatgagcg
     7981 tgatcgtctt catcaagcac cgcgccaaca tcgcccggct gatggcgggc acagagggcc
     8041 gaatcggcaa aaagggatga aggagcgcgc cgtcgcgccg catctcagcg atcggcagcg
     8101 gcttgcctgg cttcggctga tccgcacaca gaacgttgga ccggcctcat tccgcgatct
     8161 catcaaccga ttcggttcgg cggaagccgc gcttgaaatg ctgccggaac tgatgatctc
     8221 tggcggcctg aacaggctcg tccgcattcc aacggtcgcc gaggcggaag ccgaaatgga
     8281 agccgccgga aaggccggcg cgcgtttcgt cggcatcggc gaaccgggct acccgccgct
     8341 tttgcgaaac atcgaccatc cgccgccact gcttgcgctg aaaggcgatg gcgctgtttt
     8401 cgacctgccg gcgatcgcca tcgtcggcgc gcgcaacgct tcgcttgccg gcatcaagat
     8461 ggcgcggacg ctggccgccg aacttggccg gcaaggatat gccatcgtct cgggcctggc
     8521 gcgcggcatc gacacggcgg cccaccaggg aagcctggcg accggcaccg tcggcgtgtt
     8581 ggcgggcggg ctggatatgc cctacccgcc tgagaatacc ggccttttac acgacatctg
     8641 cgaacatggc ggcgccgtcg tctccgaaat gccattcggc tggcagccgc gcgcgcagga
     8701 ttttccacgc cgcaatcggc tcgttgccgg cgcggctttc gggctggtcg tggtggaagc
     8761 ggcaaagcgc tccggctcgc tgataagcgc aaggctggcc ggcgaaatgg gacgcctggt
     8821 gttttccgtg ccgggatcgc cgctcgaccc gcgcgccggg ggaaccaacg ggcttttgaa
     8881 ggatggcgcc attctcgtca ccgaagcggc ggatgtgctg gaagctatcg caccgctgac
     8941 cggcgcgccc tcaagaacca gcctggagga gccgccggac ttctcggcga cgccaccgcc
     9001 gggcgaaggc gaccgggacc tcgtcatcga ggcgctaggg ccgacgccgg tatcgcctga
     9061 cgagatcatc cgccataccc gcctgcatcc cgcgcaggtg ttcatggtgc tgctcgaact
     9121 cgatcttgcc ggcaggcttg aacgccatgc cggcggcagc gtctcgctgg ttttctagaa
     9181 acgccccaaa aggcctgtgg tgggcggggt gggtatcgaa cccacgacca ttcgattaaa
     9241 agtcgaatgc tctaccactg agctacccgc ccgcctcgct taatcgccgt gcagcaagcc
     9301 ggcgcgcggc ttcgattgcg acgcacacgc cgtcggcatg catccatgca caattggcgg
     9361 aattgcgagc tttcgccggc caaacaaggc cggatgaaag tgggcggaac atagggagag
     9421 tgcccttcgc ggtcaaccgg aaaaagaagg ccccgcggca gccgtttggc gcgccacggc
     9481 cgcttgggcg cgcacaaccg ggccttgcaa acgaagaaga cggcatatgt taaaaagcgg
     9541 cgctaagcca tgacgcttta ttaaattccc gtaactatga aaaggttgag ggaaccaata
     9601 tgttcgcctt ctcgttgatg caccacaagg gaccagaacc ttttttgagg agcagattat
     9661 gaacaagatt gtatccggcc ttttggccac ggttttatcc atttcgtttg tcggcgcgga
     9721 aatgctgccg gtaaacgccg catccatcta tgttccgcag acctcgactg ccatttcttc
     9781 ggagatcgtg cctgtggtcg acaggggcgg cggcaatttc aaccgaggcc gcgtgttccg
     9841 ccacggttcg cgcaattcat ggagcggcaa ccgctcgtgg aatggaaacc gcaactatcg
     9901 cggtggcggc cgcggctact ggaacggaca ccgcggctcc agctattatc gtcgcggcta
     9961 caggcgtcac ggcaacctct ggttcccgct ggctgcgttt gccacaggcg cgattatttc
    10021 gggcgcgatc gccaatgacc gcccgccagc ctatagcggc aattcgcacg tccagtattg
    10081 ctacaaccgc tacaagtcct atcgggcctc ggacaataca ttccagccct ataacggtcc
    10141 gcgtcagcag tgccgttaag cccgaccaag aaaacgattg attgagaaag cccgcgcaag
    10201 caattgcgcg ggcttcttat tttagcgcgc gcttgcggtg cgcctcaccg catcacgcca
    10261 gcctttcagc ttggccgtcc gctggccggc atccattttc ggctcgaagc ggcgatccag
    10321 cgcccagctt tcggcgaatt cttcggcctg cggccacata ccggcgcgcg aaccagccag
    10381 ccaggctgcg ccgagagccg tggtttcgag gatcgtcggc ctgtcgaccg gcgcatcgag
    10441 aatgtcggcc aaccgctgca tggtccaatc cgaggcgacc atgccgccat cgacccgcaa
    10501 taccgtcccg gtcgtttcgc ccttccagtc cctgcgcatg gcatcgagca ggtcgccgct
    10561 ctggtaagct accgattcga gcgcggcgcg ggccagttcc ggccgccccg aattgcgcgt
    10621 caatccgaaa atggcgccac gcgcatcggc gtcccagtgc ggcgcgccaa ggccaacgaa
    10681 cgccggcacc agataaacat cctgcgccgg atcggccttg gccgccagca ggcccgcctc
    10741 gccggcccga tcgatgatct tcaacccatc gcgcaaccat tgtacggctg ctccagcgat
    10801 gaatatcgac ccctccagcg cataggtggt gcgcccttcc agccggcagg cgatggtcgt
    10861 cagtagccgg ttcttcgagc gcacgatatc cgtgcccgta ttgagcaacg cgaagcagcc
    10921 ggtgccatag gtggatttca tcatgcccgg cttaaagcat gcctgcccga tggccgctgc
    10981 ctgctggtca ccggccacgc ccaaaatcct gatgccggag ccaaagaggc ttttgtcggt
    11041 gacgccgaaa tcgtcggcgc agtccttcac ctgaggcagc atcctggctg gaatatccaa
    11101 tattccaagc agttccttgt cccactcgtt cttttcgatg ttgtagacca gcgtgcgcga
    11161 ggcgttggtg gcgtcggtgg cgtgcatcgc gccgccggtc agccgccaga tcaggaaact
    11221 gtcgatggtg ccggccagca gttcgccgcg ctcggcgcgt tttctcgcgc ccttcacctt
    11281 gcccagcagc cacgcgattt tggttgccga aaaataggga tcgagcagaa gcccggtctt
    11341 cttcgtgaat tttttttcca ggccctgctt tttcagtttc tggcaaaggt ttgccgtcct
    11401 gcggtcctgc catacgatgg ctggatggat ggcttttccg gtttcacggt cccagacgac
    11461 aattgtctcg cgctggttgg ttatgccgat cgccgctact tcagaggctt ggcgcccggc
    11521 cttcttcagc gccgccttga ccgaccagac gacgctggac cagagttctt cagggtcatg
    11581 ctcgacccag ccagaggccg gataaaattg cgcaaattcc ttctggccaa tgcccgcgac
    11641 cctcatatgt ccatcgaaca ggattgcgcg ggtcgatgtc gttccctggt cgatcgccag
    11701 cacaaatccc gtcatcttcg ctcccgatgg acagattacc gcatgttcag ggttcgcatg
    11761 aaatcttcca gcgccgccac ctgcccggcg tcgaaatgca ggccgcgctt ggtgcgccgc
    11821 cacagcacgt cctcggcggt cattgcccat tcgtggccgg ccagatagcg cacctcggcc
    11881 tcatggaggt cgccgccgaa atcgcggccg agatcggcct gcgagcgggc ctcgcccagc
    11941 aacagccgcg cgcgggtgcc gtagaggcgg gtcagccggc gcgccagccg ctggtccaga
    12001 aacggatagg ccgctttgag gccccgcacc tcggcatcga acccggtggc cgggaaatcg
    12061 ccgccgggca gcggcgcgtt ggccgtccat gccttgccgc gcttgccaag aaagccctcg
    12121 atcttctcca gcatcgattc cgacaggcgg cgataggtgg tgatcttgcc gccaaaggca
    12181 tcgacgatcg gcgcccgccc atcgccgccc tcgcccttca gcacatagtc gcgggtcgcc
    12241 tcctgggctt tcgacgcgcc gtcatcataa agcggccgga cggcggaata ggtccataca
    12301 atgtctgcgc gcctgaccgg ctccgcgaaa tattcgctcg cggcggcaca gagataatcg
    12361 atttcggcgt cgctgatttt cacggcatga gggtcgccgt caaagtcccg gtcggtggtg
    12421 ccgatcagag tgaaatcttc ctcgtaaggg atcgcaaaga tgatacggcc gtccttattc
    12481 tgaaagaaat aggcgcgcgg atcttcgaat ttcctgcgca ccacgatgtg gctgccctgc
    12541 acgaggcgaa cattgtggac gtcctgccgc ccgaccacgc ccgacagcac atgatcgacc
    12601 catggcccgg ccgcattgac gagcaggcga gccttgacct cctcggtgcc gcc
//
LOCUS       DASYCS010000081         3938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_450626,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000081 DASYCS010000000
VERSION     DASYCS010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450626"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="VGM46_02880"
     CDS             complement(<1..860)
                     /locus_tag="VGM46_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191567.1"
                     /translation="MRDENRSGNSAALSRGLPGSRVAFRWMAISGAGIGLALLSTATV
                     AGLVFLGASIASPPHFSADFSKAQQAAMPDRLMLATTLSSPRHGDCQFGCTQRAQYIV
                     QQAKSPRTARKADAQLEADAAMSPRAKRRFDKARALLTHGKLAAAFARSTTQMAELNT
                     ALPSPLKNRFYPAGAKAVSLLDGAGPAATRFGPQVAEVERSSRLALALAGSAEIQVAY
                     ATDAAALVGPSMPVLLQDTAQEEASVETADAPVFELVPDSAPLPARRPKMESQMAAIV
                     ETAPRQPAIVA"
     gene            complement(1191..3896)
                     /locus_tag="VGM46_02885"
     CDS             complement(1191..3896)
                     /locus_tag="VGM46_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019859053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY4191568.1"
                     /translation="MTPGFDDIPFFDEEPAPRKPPAATGAPAAPAGGIAARAMAARSG
                     RNSAPDYLKGLNPEQRLAVETTEGPVLVLAGAGTGKTRVLTTRIAHILATGKAFPSQI
                     LAVTFTNKAAREMKSRIGLLVGEAIEGMPWLGTFHSIGVKLLRRHAELAGLRSDFTIL
                     DTDDVVRLIKQLIQAEGIDDKRWPAKQFAQMIDGWKNKGLGPADIPEGDARAFANGKG
                     RELYKAYQDRLLTLNACDFGDLLCHPIRIFRAHHDVLAEYHRRFKYILVDEYQDTNTA
                     QYMWLRLLAQRPKSSGDLPAEGRGGDRRPPDFSGAPAQASEQSERSAVSERAPASENK
                     INICCVGDDDQSIYGWRGAEVDNILRFEKDFPGAAVIRLERNYRSTAHILGAASHLIA
                     HNEGRLGKTLFTDRPDPEDPKVNVHAAWDSDEEARAIGETIEAHQRAGHALNDMAILV
                     RASFQMRSFEDRFIEIGLNYRVIGGPRFYERQEIRDAMAYLRVVAQGADDLAFERIVN
                     VPKRGLGEAAIRQIHDTARALRIPMLEAAGNLAESDELKPKPRAALREITANFARWQA
                     ALDNTPHNELAETILEESGYTDMWKNDRSAEAPGRLENLKELIRSMEEYESLRSFLEH
                     VALVMDAEQNESLDAVSIMTLHSAKGLEFETVFLPGWEEGLFPHQRALDEGGRSGLEE
                     ERRLAYVGLTRAKKNLHLWFVSNRRIHGLWQSTIPSRFLDELPEAHVEVQEGGNSYGG
                     YGNPYGGSSFASGRGGGSGGRQNPYGASRFDKLGETGGGEKSGAFSNTYATPGWQRAQ
                     ANRTEATSRNWGTRSGHQVERIGYGETDSGYGAGRGSVKGRTIDGELVAKSVADTPSA
                     FTVGDRVFHQKFGNGNVAAIEGNKLTIDFDKAGQKKVLDGFVTGI"
BASE COUNT          672 a         1190 c         1336 g          740 t
ORIGIN      
        1 gccacgatag cgggctggcg cggtgcggtt tcgacgatgg ccgccatctg gctttccatt
       61 ttcgggcggc gagccggaag cggcgcgcta tcgggcacca gctcgaagac gggcgcatcg
      121 gcggtttcca ccgaggcttc ctcctgcgcc gtgtcctgca acagcacagg catcgagggg
      181 ccgaccagcg cagcggcgtc ggtagcatag gccacctgaa tttcagcgga accggcaagg
      241 gccagcgcga ggcgcgacga acgttcaacc tcggcgacct gcgggccgaa gcgggtggcg
      301 gcggggccgg cgccgtccag caaggaaacg gccttcgcac cggcagggta gaaacggttc
      361 ttcagcgggg aaggcagggc ggtattcagt tccgccattt gggtcgttga gcgggcgaat
      421 gccgcggcga gcttgccatg ggtcaataaa gcccgcgcct tgtcgaagcg acgcttcgcg
      481 cggggcgaca tggcggcgtc ggcttccagc tgggcatcgg ccttgcgcgc cgttcgcggc
      541 gatttcgcct gctggacgat gtactgcgcg cgctgcgtgc agccgaactg gcaatcaccg
      601 tggcgtggcg acgacagcgt cgttgccagc atcagccgat ccggcatggc cgcttgctgc
      661 gccttggaaa agtcggcgga gaaatgggga ggcgaagcga tggacgcgcc aaggaatacg
      721 aggccggcaa cggtcgccgt cgaaaggagc gccaggccga tgcccgcacc cgatatcgcc
      781 atccagcgga atgcgacccg cgatccgggc agccctctgg acaaggcagc ggaattgccg
      841 ctgcggtttt catcacgcat aaacgccata cgaccaactc ttctcaaccg gcaaacacac
      901 gcatcgccac aggcgcgaag aaaacccttc ttcgcaggtg cagatttgcc accggttgaa
      961 tcaaaggatg ggcaaagatg gttaaccaat ccctgtacca atcactgttt ttgggtcccg
     1021 cggcccgttt ttttggatgc tcccctcgtc ggaaaagaag gcgttaaaac cccttccggg
     1081 gtctcaactg cctatttttg ccggcggagt caagaaagtg cggttttttc gtcacgcacc
     1141 cgaaaaaacg atcggacgaa aaaaagattt tgtggatggc agggcttcgt tcagatcccg
     1201 gtcacaaagc cgtccagaac cttcttctgg ccggctttgt cgaagtcgat cgtcagcttg
     1261 ttgccttcga tggcggcaac gttgccgttg ccgaatttct ggtggaaaac gcggtcgccg
     1321 acggtaaagg ccgacggggt gtcggccacg gacttggcca ccagctcgcc gtcgatggtg
     1381 cggcccttta ccgagccgcg gccggcgcca tagcccgaat cggtttcacc gtagccgatg
     1441 cgctcgacct ggtggccgga acgcgtgccc cagttacggc tggttgcttc ggtgcggttg
     1501 gcctgggcgc gctgccagcc cggcgtggca taagtgttgg agaacgcgcc cgatttttcg
     1561 ccgccgccgg tctcgcccag cttgtcgaag cgcgatgcac cgtaagggtt ttgccggccg
     1621 ccggagccgc cgccgcgtcc cgaagcgaag gacgagccgc cgtagggatt gccgtagccg
     1681 ccataggaat tgccgccttc ctgcacctcg acatgggctt caggcagttc atcgaggaag
     1741 cgcgacggaa tggtcgattg ccataggccg tggatgcggc ggttcgaaac gaaccacaga
     1801 tgcaggttct tcttggcgcg cgtcaggccg acataggcca gccggcgttc ttcctccagc
     1861 ccggaacggc cgccttcatc cagcgcgcgc tggtgcggga acaggccttc ttcccagccg
     1921 ggcagaaaga ccgtttcgaa ttcgaggccc ttggccgagt gcagcgtcat gatggagacg
     1981 gcgtcgaggc tttcgttctg ctcggcatcc atgaccaggg cgacatgctc aaggaaggag
     2041 cgcagcgatt cgtactcctc catcgagcgg atcaactcct tcaggttttc cagacgtccc
     2101 ggtgcttccg ccgagcggtc gttcttccac atgtcggtat agccggactc ttcaaggatc
     2161 gtctcggcca gttcgttatg cggagtattg tcgagcgctg cctgccagcg ggcgaaatta
     2221 gcggtgatct cgcgcagcgc ggcgcggggc ttgggcttca gctcgtcgga ttcggcaagg
     2281 tttcccgccg cctccagcat cggaatgcgc agcgcgcgcg ccgtgtcatg gatctggcgg
     2341 atggcggctt cgcccaggcc gcgcttcggc acgttgacga tgcgctcgaa ggcgaggtcg
     2401 tcggcgccct gggcgacgac gcgcagatag gccatggcgt cgcggatttc ctggcgctca
     2461 taaaagcgcg ggccgccgat gacgcggtag ttcagaccga tctcgatgaa gcggtcttcg
     2521 aaggagcgca tctggaaaga ggcgcgcacc aggatcgcca tgtcgttgag cgcgtgcccg
     2581 gcgcgctgat gggcctcgat ggtttcgccg atggcgcggg cttcctcgtc ggaatcccat
     2641 gcggcatgga cattgacctt ggggtcttcc gggtccggac gatcggtaaa cagcgtcttt
     2701 cctagccgtc cctcattatg ggcgatgagg tgcgaggccg cgcccagaat gtgggcggta
     2761 gagcggtagt tgcgctccag ccggatgacg gcggcgcccg gaaaatcctt ctcgaagcgc
     2821 aggatgttgt ccacctccgc gccgcgccag ccataaatcg actggtcgtc gtcgccgacg
     2881 cagcagatgt tgattttgtt ttcgctcgcg ggggctctct cgctcacggc cgagcgctcg
     2941 ctttgctcgc tggcctgcgc gggcgcgccc gaaaagtcgg gcgggcggcg gtcgccgcct
     3001 cggccttcgg ccggaaggtc gccgctggat ttcggccgct gcgccagcag gcgcagccac
     3061 atatattgcg ccgtgttagt atcctggtac tcgtcgacga ggatatattt gaagcggcgg
     3121 tgatattcgg ccagcacgtc gtgatgggcg cggaaaatgc ggatcgggtg gcagagaagg
     3181 tcgccgaaat cgcaggcgtt cagcgtcaaa aggcgatcct gataggcctt gtagagttcg
     3241 cgacccttgc cgttggcgaa ggcgcgggca tcgccttcgg gaatgtcggc ggggccgagg
     3301 cccttgtttt tccagccgtc gatcatctgc gcgaactgct tggctggcca gcgcttgtcg
     3361 tcgatgcctt cggcctggat cagctgcttg atcaggcgca ccacatcgtc ggtgtcgagg
     3421 atggtgaagt ccgagcgcag gccggcgagt tcggcgtgac ggcgcaaaag cttgacgccg
     3481 atggagtgga aggtgccgag ccacggcatg ccctcgatcg cctcgccgac gagaaggccg
     3541 atgcggctct tcatctcgcg cgccgccttg ttggtgaagg tcacggccag gatctgcgac
     3601 gggaacgcct tgccggtggc aaggatgtgg gcgatgcgag tggtcagcac gcgggtcttg
     3661 ccggtgccgg cgccggccag caccagaacc gggccttcgg tggtttccac cgccagcctt
     3721 tgttccgggt tcaacccctt gagatagtcg ggcgcgctgt tgcggccgct gcgggcagcc
     3781 atggcgcgcg cggcaatgcc ccctgctgga gcggcaggag cgcccgttgc ggccggcggt
     3841 ttgcgcgggg cgggctcttc gtcgaaaaag ggaatgtcgt caaatccggg cgtcatgcgg
     3901 ctcaatgtag tgattcggtc ctgaaaggca aaaggaag
//
LOCUS       DASYCS010000082         3292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3930320,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000082 DASYCS010000000
VERSION     DASYCS010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3930320"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VGM46_02890"
     CDS             complement(<1..222)
                     /locus_tag="VGM46_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014766378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HEY4191569.1"
                     /translation="MAGDVTGIEPKGIEVVTFGCRLNAYESEVMRREAEAAGLGALAG
                     GAIIVNTCAVTGEAVRQAKQSIRKARRENP"
     gene            complement(222..1103)
                     /gene="dapF"
                     /locus_tag="VGM46_02895"
     CDS             complement(222..1103)
                     /gene="dapF"
                     /locus_tag="VGM46_02895"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEY4191570.1"
                     /translation="MTVEAPFAKMNGIGNEIIVADMRGRVDRVTPAAAIALNADAATR
                     FDQIMAIHDARTPGTGFFIDILNSDGSPAQACGNGTRCVVQALAAETGETAFTFETVA
                     GILNANQRDDGTISVDMGMPRFGWQEIPLAEEFRDTRMIELQIGPIDAPVLHSPSVVS
                     MGNPHAIFWVDRDVWSYDLDRFGPMLENHPIFPERANITIAQVTSPETMTIRTWERGA
                     GLTKACGSAACAAAVAAARTRRTGRSVTLQAPGGMLQVDWREDDHVVLTGAAEWEFSG
                     TFDPATGAFARDAESVA"
     gene            complement(1175..1348)
                     /locus_tag="VGM46_02900"
     CDS             complement(1175..1348)
                     /locus_tag="VGM46_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019997827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 family protein"
                     /protein_id="HEY4191571.1"
                     /translation="MIKWIIILLIVSVIAGALGFRGVAGTTSRIASFLIAVALVIVAV
                     VLLLFAWAGGGVW"
     gene            1555..2094
                     /locus_tag="VGM46_02905"
     CDS             1555..2094
                     /locus_tag="VGM46_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AprI/Inh family metalloprotease inhibitor"
                     /protein_id="HEY4191572.1"
                     /translation="MTFSKTGLMAMMLAALLASGCSSTRLSSMDSTQPAPLEAAPAGN
                     VTSNQLPPPSGPTGTDPSQFPTAPQNTQVASLPADGSAPATAADLTASSVAGVWNASV
                     SGQSCKVATPQTKFGQGYRAGPLRCPAPLDGVKSWNVAGKQLTLYDNDGGTLARLYSS
                     GGEKFDGQTSTGLPIALTR"
     gene            2124..>3292
                     /gene="zapE"
                     /locus_tag="VGM46_02910"
     CDS             2124..>3292
                     /gene="zapE"
                     /locus_tag="VGM46_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HEY4191573.1"
                     /translation="MHLRDGLQTRATVRQRYDHQVSTGAIERDPAQERIATALDRLIE
                     EIAAKRISHKSSSLGWLFARKKPQEAVKGLYIHGSVGRGKTILMDMFFELAPVRRKRR
                     VHFNDFMADVQDRIQKHRQARKEGTVKEDDPIPPVARALADEAWVLCFDEFSVTDIAD
                     AMILSRLFSALFANGVVLVATSNVAPLDLYRDGLNRQLFTPFIAMLERHANVVSLDAE
                     KDYRLEKLARTPVYLTPVDDAAERTMDETWTAMTYGAAAAPEILTVKGRNLQVPVAAG
                     KAARFSFADLCEAPLGARDYKVLADRFDTFFVDHVPVLGSTLRNATKRFILLIDTLYD
                     QGKRLVVSAAAAPDNLYAGRAATTEAFEFDRTASRLIEMQSRDWLEAWAERQDKQG"
BASE COUNT          601 a         1100 c         1034 g          557 t
ORIGIN      
        1 cggattttcg cgccgcgcct tgcggatcga ttgcttggcc tgccgcacgg cctcgccggt
       61 aaccgcacag gtgttgacga taatggcgcc gcccgcaagc gcgccaagcc cggcggcctc
      121 ggcctcgcgc cgcatcactt ccgattcgta agcgttaagc cggcaaccga aggtgacgac
      181 ttcgatgccc ttcggctcga tgcccgttac atcgcccgcc atcaggcaac gctctctgcg
      241 tctcgcgcga aagcgccggt cgccgggtcg aacgtgccgg aaaactccca ttcggccgca
      301 ccggtcaaaa ccacatggtc gtcttcgcgc cagtcgacct gaagcattcc gccgggggcc
      361 tgcaaggtga cgctgcggcc ggtgcgcctg gtgcgcgcgg cggcgaccgc ggcggcgcaa
      421 gcggccgaac cgcacgcctt ggtcagcccc gccccgcgct cccaggtgcg gatcgtcatc
      481 gtttcgggcg acgtcacctg cgcgatggtg atgttggcgc gttcggggaa gatcgggtgg
      541 ttttcgagca tcggcccgaa gcgatcaaga tcataggacc agacatcgcg gtccacccag
      601 aaaatggcgt gcggattgcc catcgacacc accgagggcg aatgcagcac cggcgcatcg
      661 atcgggccga tttgcagttc gatcatgcgg gtatcgcgaa attcctcagc cagcggaatt
      721 tcctgccagc cgaagcgcgg catgcccatg tcgacggaaa tggtgccgtc gtcgcgctgg
      781 ttggcgttca ggatgccggc gacggtttcg aaggtgaagg cggtttcgcc ggtttcggcg
      841 gccagtgcct gcaccacgca gcgtgtgcca ttgccgcaag cctgcgcggg cgaaccgtcg
      901 gaattcaata tgtcgatgaa gaagccggtg ccgggcgtgc gcgcatcgtg aatcgccatg
      961 atctggtcga agcgggtcgc cgcatcggca ttgagcgcga tggcggccgc cggcgtcacg
     1021 cggtcgacac ggccgcgcat gtcggcgacg atgatctcgt tgccgatgcc gttcatcttg
     1081 gcgaaggggg cctcgactgt catggcgatg gaaattctct tgtcggtttg cgcctatatg
     1141 gcggaaacgg ccatgaatta ccagtgcggc ggccttacca aacgccgccg cccgcccagg
     1201 cgaacagcag cagcaccacc gccacgatca ccagcgccac cgcgatcagg aacgaggcga
     1261 tgcgcgaggt cgtacccgcc acgccgcgaa aacccaatgc gccggctatc accgaaacga
     1321 tcaggagaat gatgatccat ttaatcatgc cgggttcccc gctggtttgc gccgggataa
     1381 cgcggcggac ccgtgcccgt tccccgaaag aaccgttttc aggcggcaaa aatcgacaca
     1441 gattccagcg caaaccgtat tttaaccata tcgggttgaa attctgccgc cctgcccatg
     1501 ttggtaggat aagacttatg gattgagcgc ccgcagcggg tcgcggaggt tggcatgaca
     1561 ttctcgaaaa ccggtctgat ggcgatgatg ctggcggccc ttttggcaag cggctgctca
     1621 agcacgcgcc tgtcgtcgat ggattccacc cagccggccc cgcttgaggc ggcgccggcg
     1681 ggcaatgtca catcgaacca gttgccgccg ccctccggcc cgaccggcac ggacccttcg
     1741 caattcccca ccgcgccgca gaacacgcag gtagcctcgc tgcctgccga cgggtccgcg
     1801 cccgccacgg ccgccgatct caccgcctcc agcgtcgccg gcgtgtggaa tgccagtgtt
     1861 tccggccaga gctgcaaggt ggcgaccccg cagaccaagt tcggccaggg ctaccgcgcc
     1921 gggccgctgc gctgccccgc cccgctcgat ggcgtcaagt cgtggaacgt cgccggcaag
     1981 cagctgaccc tctacgacaa tgacggcggc acgctggcgc gcctctattc ctcgggcggc
     2041 gaaaaattcg atggccagac ctcgaccggc ctgccgatcg cgctgacgcg ataatcgacg
     2101 catccaggcg gcggcgataa aagatgcatt tgcgtgacgg cctccagacc cgtgcgaccg
     2161 tcaggcagcg ctacgaccat caggtttcga ccggagccat cgagcgcgac cccgcgcagg
     2221 aacgcatcgc caccgcgctc gatcggttga tcgaggagat agcggcaaag cgcatttcgc
     2281 ataaatccag ctcgctgggc tggctttttg cccgcaaaaa gccgcaggaa gccgtaaagg
     2341 gcctctacat tcacggcagc gtcgggcgcg gcaagaccat actcatggac atgttctttg
     2401 aactcgcgcc ggtgcgccgc aagcgccgcg tccacttcaa cgacttcatg gccgatgtgc
     2461 aggaccgcat ccagaagcac cggcaggcgc gcaaggaagg aaccgtcaag gaagacgatc
     2521 cgatcccgcc ggtggcgcgg gccttggccg acgaggcctg ggtgctgtgc ttcgacgaat
     2581 tttccgtaac cgacattgcc gacgcgatga tcctgtcgcg gctgttttcg gcgctgtttg
     2641 ccaacggcgt cgtcctggtc gccacctcca acgtcgcccc gctggacctt taccgggacg
     2701 ggctgaaccg gcaattgttc acgcccttca tcgccatgct ggaacggcac gccaatgtcg
     2761 tctcgctcga tgccgaaaag gactaccggc tggaaaaact ggcgcgcacc ccggtctatc
     2821 tgacgccggt tgacgatgcc gccgaacgca ccatggacga gacctggacg gcgatgacct
     2881 atggcgcggc ggctgcgccc gaaatcctga ccgtcaaggg ccgcaatctt caggtgccgg
     2941 tagctgccgg caaggccgcc cggttttcct tcgccgatct gtgcgaagcc ccgctcggcg
     3001 cgcgcgacta caaggtgctg gccgaccggt tcgacacttt cttcgtcgat cacgtgccgg
     3061 tgctcggctc gacgctgcgc aacgccacca agcgcttcat cctgttgatc gacacgcttt
     3121 acgaccaggg caagcggctc gtcgtcagcg ccgccgctgc gcccgacaat ctctatgccg
     3181 gacgggcggc gacgaccgag gccttcgagt tcgaccgcac cgcatcgcgg ctgatcgaaa
     3241 tgcaaagccg cgattggctg gaagcctggg cggagcggca ggacaagcag gg
//
LOCUS       DASYCS010000083         1768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_597084,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000083 DASYCS010000000
VERSION     DASYCS010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597084"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VGM46_02915"
     CDS             complement(<1..257)
                     /locus_tag="VGM46_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HEY4191574.1"
                     /translation="MAVLPTGVRHMPAYFSPEVQEALLDAIREIVQQAPLYVPAMPRT
                     GKEMSVRMTNCGTLGWVTDKERGYRYQPTHPVTNLPWPPIP"
     gene            complement(333..770)
                     /gene="arfB"
                     /locus_tag="VGM46_02920"
     CDS             complement(333..770)
                     /gene="arfB"
                     /locus_tag="VGM46_02920"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013891634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEY4191575.1"
                     /translation="MSGDDKIIISGEAVIHPDDLHEDFIRSSGPGGQNVNKVATAVQL
                     RFDAANAPGLSERVRERAIKLAGQRATKDGVIVIEAGRFRTQEQNRADARARLAALVG
                     RAAEPPPPPRKKTRPTKGSVERRLKSKAGRSTVKKLRGRVGDD"
     gene            complement(854..>1768)
                     /locus_tag="VGM46_02925"
     CDS             complement(854..>1768)
                     /locus_tag="VGM46_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012091053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEY4191576.1"
                     /translation="IFFGLSGTGKTTLSADPSRTLVGDDEHGWGPHGIFNFEGGCYAK
                     TIRLSAEAEPEIFAATQRFGTVLENVILDEDRVPDFDDGSLTENTRCAYPLDFIPNAS
                     ASGRAGHPKNIILLTADAFGVMPPVARLTPAQAMYHFLSGYTAKVAGTEKGVTDPEAT
                     FSTCFGAPFMPRHPSEYGNLLRELIAKHNADCWLVNTGWTGGAHGTGKRMPIKATRAL
                     LGAALDGSLGNAEFRTDANFGFEVPVAVPGVDGAILDPRSTWADKAAYDRQAARLVSM
                     FIANFEKFQSHVDGPVRDAAPNIRQAAE"
BASE COUNT          292 a          542 c          602 g          332 t
ORIGIN      
        1 tccgggatcg gcggccacgg caggttggtc acggggtggg tcggctggta gcggtagccg
       61 cgctccttgt cggtgaccca gccgagcgtg ccgcaattgg tcatgcgaac gctcatttcc
      121 ttgccggttc gcggcatggc cggaacgtag agcggtgcct gctggactat ttcgcggatc
      181 gcgtcgagca gcgcttcctg cacctctggt gagaaatagg ccggcatgtg gcgaacgccc
      241 gtgggcaaaa cggccatggc agaatcctta ccgtcgaaac agcggcacga tggcaaagcc
      301 gggctgtggc ggcaacccga aagcgtttcg gctcaatcgt cgccaacccg cccgcgcaat
      361 tttttcacgg tggagcggcc ggccttgctt ttcagccgcc gttcgacaga gccttttgtc
      421 ggccgcgtct tcttgcgcgg cggtggcggc ggctcggcgg ccctgccgac cagggcggcg
      481 agccgcgcgc gggcatcggc gcggttctgc tcctgcgtgc ggaaccgccc ggcttcgatg
      541 acgatgacgc cgtccttggt ggcgcgctgg ccggccagct tgatggcgcg ctcgcgcacc
      601 cgctcggaaa gaccgggagc gttggctgcg tcgaagcgca actgcaccgc cgtcgccacc
      661 ttgttgacgt tctgcccgcc tggacccgag gagcggatga aatcctcgtg caaatcgtct
      721 ggatggatga ccgcctcgcc ggaaatgatg attttatcgt cgccgctcat gccgcagtca
      781 tggcgcgggc gatgaaaaaa gaaaaggccc gatcgaagcc gggccttttt gcgaagtgcg
      841 agccttgtcg gaattattcg gcggcttgcc ggatgttcgg tgcggcgtcg cgcacaggtc
      901 catcgacgtg gctttggaat ttttcgaaat tggcgatgaa catcgaaacc agtctggcgg
      961 cctgccggtc ataggcggcc ttgtctgccc atgttgagcg cgggtcgagg atggcgccgt
     1021 cgacgcctgg caccgcgacc ggcacttcga agccgaaatt ggcatcggtg cggaactcgg
     1081 cattgccgag cgaaccgtcc agcgcggcgc caagcagcgc gcgtgtcgcc ttgatcggca
     1141 tgcgcttgcc ggtgccgtgg gcgccgccgg tccagccggt attgaccagc cagcaatcgg
     1201 cattgtgctt ggcgatcagc tctcgcagca gattgccgta ttcggaaggg tggcgcggca
     1261 tgaacggcgc gccgaagcag gtcgaaaagg tcgcttcggg gtcggtcacg cctttctcgg
     1321 ttcccgccac cttcgccgtg tagccggaga ggaagtgata catcgcctgc gccggcgtca
     1381 acctggccac cggcggcatg acgccgaagg catcggccgt caacaggatg atgttctttg
     1441 gatgcccggc gcggccactg gcgctggcat tgggaatgaa gtcgagcggg taggcgcagc
     1501 gcgtattttc ggtcaggctg ccatcgtcga agtccggcac gcggtcctca tcgagaatga
     1561 cattttccag caccgtgccg aaacgctgcg tcgcggcgaa gatttccggt tcggcttccg
     1621 cagaaagccg gatcgtcttg gcgtagcagc cgccttcgaa attgaagatg ccgtgcggcc
     1681 cccagccatg ctcgtcgtcg ccgaccagcg tgcgcgacgg atcggccgaa agcgtcgtct
     1741 tgccggtgcc ggaaaggccg aagaaaat
//
LOCUS       DASYCS010000084         3044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3060967,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000084 DASYCS010000000
VERSION     DASYCS010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3060967"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VGM46_02930"
     CDS             <1..240
                     /locus_tag="VGM46_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEY4191577.1"
                     /translation="RDLIENAEAKLKKKGADFIVANDVSLASGIGGGGVMGGDRNRVR
                     IISKAGVEEWPEMSKDEVSARLAGLIAQKLRTVAI"
     gene            complement(263..739)
                     /locus_tag="VGM46_02935"
     CDS             complement(263..739)
                     /locus_tag="VGM46_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF992 domain-containing protein"
                     /protein_id="HEY4191578.1"
                     /translation="MKMKLACASALLATMIMAAPASAGSLELGVLDCTIDGGTGYLIA
                     SNKGVACTFRPHNGRASEAYTGVISKIGVDLGITHQGALQWAVLAATKDGPEDKLAGN
                     YVGVNAEASVVTGGGANLLVGGFHDSITLQPLSVQAQTGLNLAVAVTSIELIRSFK"
     gene            complement(952..1800)
                     /locus_tag="VGM46_02940"
     CDS             complement(952..1800)
                     /locus_tag="VGM46_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006339043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY4191579.1"
                     /translation="MNASTDMRRIAPRGLVIAAALFSGLALAGCQSGPAGELATIDTA
                     QGSSENIASLSSVIESNPRDPESYNVRGSAYGRGGKYQAALKDFNTAIQINPNFYQAY
                     SNRALIYRFLGDQTSALNDYNKSIQINANYDAAYIGRGNLYRKAGRTKEAFADFQKAI
                     QLDTTDARAYHNRGLIYQSQGQHNFAIEDFSTAISLSPNASEPYNGRGLSYLALNQQD
                     NAFADFNMAIKLDGKNAEAWANQALIYEQRGDKVRAAKSYREATHLNPSYQPAKDGLK
                     RTSAGS"
     gene            complement(1943..2212)
                     /gene="rpsU"
                     /locus_tag="VGM46_02945"
     CDS             complement(1943..2212)
                     /gene="rpsU"
                     /locus_tag="VGM46_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEY4191580.1"
                     /translation="MQVLVRDNNVDQALRALKKKMQREGIFREMKMRGHYEKPSEKRA
                     REKAEAVRRARKLARKRAQREGLLPMTPRPVAAGAAGAGAARPSR"
     gene            complement(2363..>3044)
                     /locus_tag="VGM46_02950"
     CDS             complement(2363..>3044)
                     /locus_tag="VGM46_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY4191581.1"
                     /translation="DLIFTPGPDEARLPVRKAPPQPMPTADLIAQLSRPKPAAEAPAE
                     PVFAIIDEAEREAGIALVMREILDDPDAAFRTDAVLYQDFLVRCRIRRVPGDPLDLAA
                     FRRKFAVARAGVDQKTAGGEAWPAALSLSEALPADLQGVFLVVAQAALAGSPCPSDAT
                     LARIYGSHSARRAARLLAYFEERNLLVARTDFHGRRVLAFPDLGCETAPGDPNAPDDV
                     PAARDAAE"
BASE COUNT          558 a          887 c         1036 g          563 t
ORIGIN      
        1 cgcgacctga ttgaaaatgc cgaagccaag ctaaagaaga agggcgccga ctttatcgtc
       61 gccaacgatg tttcactagc cagcggcatt ggcggcggtg gcgtgatggg gggcgaccgc
      121 aaccgggtgc gcatcatctc gaaggccggt gtcgaggaat ggccggagat gagcaaggac
      181 gaggtgtcgg cgcggctggc cggcctgatc gcacaaaagc tccggacggt tgccatctga
      241 caaccgccct gaggatagat cgttacttga aggagcggat aagctcgatc gaagtgaccg
      301 cgacggccag gttgagaccg gtctgcgcct gcacgctgag cggttgcagg gtgatggaat
      361 catggaagcc gccgaccaga aggttggcgc cgccgccggt caccaccgag gcttcggcgt
      421 tgacgccgac atagttgccg gcaagcttgt cttccgggcc gtccttggtg gcggcaagca
      481 ccgcccattg cagcgcgccc tgatgggtta tgccgagatc gacgccgatc ttggagatga
      541 cgccggtgta ggcttcggaa gcccgtccgt tatgcgggcg gaaggtgcag gccacgccct
      601 tgttggaggc gatcagatag ccggtgccgc cgtcgatggt gcaatcgagc acaccgagtt
      661 ccagggagcc ggcgctggcc ggggcggcca taatcatcgt tgcgagaagc gccgaggcac
      721 aggcaagttt cattttcatg ggaaatccct cgttacagta cagatgaaca atgagaaaca
      781 ccgccaaggg ttccagaaaa accatggcgc aagcatggca agggccgcgt catatggtca
      841 tgccgatggg tttgagcagc ccggaaaccg ggcgtgagcc gcagctgacg cactccagca
      901 ggaggggttg ttcctcccct cgtctgatcc ctcatctggc gtagagtcga atcagctgcc
      961 ggcgctggtc cgtttcaggc cgtccttggc cggctggtag ctcggattga ggtgcgtggc
     1021 ttcgcgatag gacttggcgg ctctcacctt gtcgccgcgc tgctcgtaga tcagcgcctg
     1081 attggcccac gcctcggcat tcttgccgtc cagtttgatg gccatgttga agtcggcgaa
     1141 ggcgttgtcc tgctggttca gcgccagata ggaaaggccg cggccattat atggctcgga
     1201 ggcgttcggc gacagcgata tggcggtcga gaagtcctcg atggcgaagt tgtgctggcc
     1261 ctggctctgg tagatcaggc cgcgattgtg ataggcgcgt gcatccgtcg tatcgagctg
     1321 gatggccttc tggaaatcgg cgaacgcttc cttggtgcgg ccggctttcc ggtaaagatt
     1381 gccacggccg atataggcgg cgtcgtaatt ggcgttgatc tggatggatt tattgtagtc
     1441 gttgagcgcg ctcgtctggt cgccgagaaa acggtagatc agggcgcggt tggaataggc
     1501 ctggtagaaa ttcgggttga tctggatcgc cgtgttgaaa tccttgagcg cggcctggta
     1561 tttgccgccc ctgccatagg ccgagccacg gacattgtag gattcggggt cgcgcgggtt
     1621 gctttcgatc accgaggaga gcgaggcgat gttctcgctc gacccttgcg ccgtatcgat
     1681 ggtggccagt tcgccggcgg ggccgctctg gcaacccgcc agcgccagac ccgaaaacag
     1741 ggccgctgca atcaccaggc cgcggggtgc gatcctgcgc atatcggtgg atgcgttcat
     1801 ctcgtcctca tcctcactgg cgccgcgccg ccgagatccg ggtccccctc cgggcctgat
     1861 caattgcaaa caaaaaggtg gcgacgatta tgcatcgcgc cacctttgaa tattcgtcga
     1921 aagggacgaa aaattggcgg tatcagcgcg aaggacgcgc agcaccggca ccggcggcgc
     1981 cagcggccac ggggcgcggc gtcatcggca gcaggccctc gcgctgggcg cgcttgcggg
     2041 ccagcttgcg ggcgcggcgc acggcctcgg ccttttcacg ggcacgcttc tcggaaggct
     2101 tctcgtaatg tccgcgcatc ttcatttcgc ggaaaatgcc ttcgcgctgc atcttcttct
     2161 tgagagcgcg gagagcctgg tcaacattat tgtcgcggac gagtacctgc acgtgcgatc
     2221 ctgtctttcg tgtttaaact cagaaggcaa gcagccatag ccacccacct ccgcggcggc
     2281 tttcaccgcg aattgcggcg tgcgatagca gaaacaacat gggctgtcca ctgaagagtg
     2341 gagaagcggt gcgattttaa cgctattcgg cggcgtcgcg ggcggcgggc acgtcatcag
     2401 gcgcgttcgg atcgcccggc gccgtttcgc agccaaggtc gggaaaggca agcacgcgcc
     2461 ggccatggaa atcggtgcgc gcgaccagaa ggttgcgttc ctcgaaataa gcaagcagtc
     2521 gcgctgcgcg gcgcgccgaa tggctgccat agatgcgggc aagcgttgcg tcggacgggc
     2581 aggggctgcc ggcaagcgct gcctgcgcca cgacaaggaa gacaccctgc aaatcggccg
     2641 gcaacgcttc ggagaggctc agtgcggcag gccatgcctc gccgccagcc gtcttttggt
     2701 cgacgccggc gcgcgcgacc gcgaatttgc ggcggaaggc cgccagatcg agcggatcgc
     2761 ccggcacgcg gcgaatgcgg cagcgaacga ggaaatcctg gtagagcacg gcgtcggtgc
     2821 ggaacgccgc gtccggatcg tccaggattt cgcgcatcac cagcgcgatg cccgcctcac
     2881 gctcggcctc gtcgataatg gcgaaaaccg gctcagccgg cgcttccgct gccggcttgg
     2941 gtcgcgaaag ctgggcaatc agatcggcgg tcggcatagg ctgcggtggc gccttgcgaa
     3001 cgggcaggcg ggcttcgtcc ggtcccggcg tgaagatcag gtcg
//
LOCUS       DASYCS010000085         5379 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2335254,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000085 DASYCS010000000
VERSION     DASYCS010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 5379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5379
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2335254"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="VGM46_02955"
     CDS             complement(<1..870)
                     /locus_tag="VGM46_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY4191582.1"
                     /translation="MLHTIAAFDRLGEENAFAVLARATALAHEGRDIINLGIGQPDFK
                     TPAHIVEAAIKALRDGHHGYTPANGLLAAREAVVRRTLATTGVEVSPESVMIMPGGKP
                     TMFAAILMFGEPGAEILYPDPGFPIYRSMIEFTGAAPIPVPMREENGFAFSAAETLAL
                     ITPQTRLLILNSPANPTGGVTPRAEIEKLVKGLETHPQIAVLSDEIYDVMTYDGAEHT
                     SLLQFPEIRDRLIVLNGWSKTWAMTGWRMGWSIWPNGEKGGHLYDKVRKLAVNCWSCV
                     NAPSQFAGIAAIDG"
     gene            938..1630
                     /locus_tag="VGM46_02960"
     CDS             938..1630
                     /locus_tag="VGM46_02960"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY4191583.1"
                     /translation="MDWSLIVPENLKDWQPRPRPERKVLEGRYVRLEPLEAKKHGDGL
                     FAASTVPDADGRFAWLFEKPPESRAEFDGWLEKVQASEDPLYFTVIDLASGKIAGRQT
                     LMRIEPNHGVIEIGSIYWGPLISRRPAATEAQYLFTRYIFDELGYRRYEWKCHNDNAP
                     SKRAAERFGFKFEGIFRQHMVTKGANRDTAWYSIIDKEWPALKKAYENWLDPSNFDSE
                     GVQKKRLEDLRK"
     gene            1669..2655
                     /locus_tag="VGM46_02965"
     CDS             1669..2655
                     /locus_tag="VGM46_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEY4191584.1"
                     /translation="MAHDHDHGSNSHGHGGGAHVHAGTDKKRVLIAACLTGGFMVAEA
                     VGGVLTGSLALLADAGHMLADSIALGLAWYAFHLAERPATGRLTYGFGRVKTLVAYTN
                     GLAIFIIALWIIYEAWQRLQAPAPVLGGPMLVVAGLGLMVNIAGFLVLHGGNRASLNM
                     RGAILHVLGDMLGSAAAIVAALVILATGWTPIDPILSVVVALLILSTAWSLMKEAGHV
                     LLEGVPPSLDRDAVASDLVANIPGLREVHHMHVWSIDGETSMATLHACMATDVDPYRA
                     VLAIKQRLRSQHNISHATVEPEFGRCTDGEGDHDTHHGHSPGHEASHSGHYH"
     gene            2719..2925
                     /locus_tag="VGM46_02970"
     CDS             2719..2925
                     /locus_tag="VGM46_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018429309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191585.1"
                     /translation="MDRDTRNAAVAALVIMVLFGAFAYYLPVIMLAAGKVSGILAAIV
                     AAVFMLALFMVFWLRSRSQRGKGD"
     gene            complement(2948..4057)
                     /locus_tag="VGM46_02975"
     CDS             complement(2948..4057)
                     /locus_tag="VGM46_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007198943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt transporter"
                     /protein_id="HEY4191586.1"
                     /translation="MKPVTRNALRSVLCLLAILVAANLFASLAHAQSSLGIGTNDGMP
                     ASTGLFAHQLLWINQQQQIFYRALANAMKAMRQDGSKLWLLVGLSFAYGIFHAAGPGH
                     GKAVISSYMVANEVALRRGILLSFISALLQAITAIVVMLLAYFVLRGTSVSMTDAAWF
                     LEISSYVFVTAFGAWLLWRKAVPALRRMSGSREANSLSAAAAHSHNGHFRGDHSQCDH
                     RPGHLNYHAHAHGEQKHQGHKHQAIAICESCGHSHAPDPALLGGERFDWRTAWTAVAA
                     VGIRPCSGALIVLSFALLNGLWVGGILSVLAMALGTAITVSALAIIAVTAKNWAVTIA
                     GDGRIGNRIHSIVEIGGAAFVFLFGLLLLSASLAA"
     gene            complement(4054..4722)
                     /locus_tag="VGM46_02980"
     CDS             complement(4054..4722)
                     /locus_tag="VGM46_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1007 family protein"
                     /protein_id="HEY4191587.1"
                     /translation="MNLKRRIPTLAAGAVSLFAAAHTAQAHPHVFAEARLDVVLNPDR
                     QTVKALRHLWRFDDLFSSTVLMEFDKNSDLKLDDAELKEVSETIFASLAEYDYFQLVT
                     VDGKDVKMKAPAHLIANFDNDQLIILFESEPEKPIKLAGKVDFGVYDPTFYTAIDFTE
                     DANMTVDDMPANCAKTVIRPDPDTAIAQNQKTLTDAFFNDPTGTDMSKIFATKLELTC
                     QAKG"
     gene            complement(4809..>5379)
                     /locus_tag="VGM46_02985"
     CDS             complement(4809..>5379)
                     /locus_tag="VGM46_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate binding domain-containing protein"
                     /protein_id="HEY4191588.1"
                     /translation="LIDFARQYPEIVLDVRLDDRFVDLVEEGFDLAIRISRLESSSLI
                     AKRLAPFSVRLCASPELIEKHGMPTRPQDLSRIPCIIDTNGRWLANWPFKGDNGDAVS
                     VPVSGPLEVNSPMAARAAAVAGLGFSILPDFIAAPDIESGKLVTALDNRILPGGGIFA
                     VYPHRRYLPVKVRVFVDFLAQWFKNMDAA"
BASE COUNT         1021 a         1569 c         1768 g         1021 t
ORIGIN      
        1 gccgtcgatg gcggcgatgc cggcaaattg cgacggcgcg ttgacgcagg accagcaatt
       61 gaccgccagc ttgcgcacct tgtcgtagag atggccgccc ttttcgccgt tcggccagat
      121 cgaccagccc atgcgccagc cggtcatcgc ccaggtcttc gaccagccat tgaggacgat
      181 cagcctgtcg cggatttcgg ggaattgcag cagcgaagtg tgctcggcgc cgtcataagt
      241 catgacgtcg tagatctcgt cggagagaac ggcgatttgc gggtgggttt ccagcccttt
      301 caccagcttt tcgatttcgg ccctcggcgt cacgccgccg gtcgggttag cgggcgaatt
      361 gagaatcagc agccgggtct gcggcgttat cagcgccagg gtttccgcag cggagaaggc
      421 aaagccgttt tcctcgcgca tgggcaccgg aatgggtgcg gcgccggtga attcgatcat
      481 cgagcgatag attggaaagc cgggatcggg gtagaggatt tccgcgccgg gttcgccgaa
      541 catcaagatg gcggcgaaca tggtgggctt gccgccgggc atgatcatga cgctttcggg
      601 cgatacctcg acacctgtgg tcgccagcgt gcggcgcacc accgcctcgc gcgccgccag
      661 aaggccgttg gcgggcgtgt agccgtgatg gccatcgcgc agcgccttga tcgccgcctc
      721 gacaatatgg gcgggcgtct tgaagtccgg ctggccgatg ccaagattga tgatgtcgcg
      781 gccttcatgg gcgagcgcgg tggcgcgcgc cagaaccgcg aaggcatttt cctcgccgag
      841 ccggtcgaaa gcggcaatgg tgtggagcat tttttgtcac ccggtggttc ttgcgtgtgg
      901 ctgtgcgtgt ttgtgaatgt cgtgcgggaa atgtcaaatg gattggagcc tgatcgtgcc
      961 ggaaaatctc aaggattggc agccgcgccc gcggcccgag cgcaaggttc tggaaggacg
     1021 ctatgtccgg ctggagccgc ttgaggccaa gaagcatggc gacggtcttt ttgccgcctc
     1081 gactgtgccg gatgccgatg gccggtttgc ctggctgttt gaaaaaccgc ccgaaagccg
     1141 cgccgaattc gatggctggc tggagaaggt gcaggccagc gaagacccgc tctatttcac
     1201 ggtgatcgac ctggcgagcg gcaagattgc cggccgccag acgttgatgc gcatcgagcc
     1261 gaaccacggc gtcatcgaga taggcagcat ctattgggga ccgcttattt cgcgcaggcc
     1321 cgcagcgaca gaggcgcagt atctgttcac gcgctacatc ttcgacgagc ttggctatcg
     1381 ccgctacgaa tggaagtgcc acaacgacaa tgcgccttcc aagcgcgcgg ccgaacggtt
     1441 cgggttcaaa ttcgagggaa ttttcaggca gcatatggtg accaagggcg ccaaccgcga
     1501 caccgcctgg tactcgatca tcgacaagga atggccggcg ctcaaaaaag cctacgaaaa
     1561 ctggctggac cctagcaact tcgacagcga aggcgtacag aaaaagcgcc ttgaggattt
     1621 acgtaagtga ttgaggcgta aggttatttc tttacgcgag aggtgatgat ggcacacgac
     1681 cacgatcacg gttcgaacag ccatggccat ggcggcggcg cgcatgtcca tgccggcacc
     1741 gacaagaaac gcgtgctgat cgccgcctgc ctgaccggcg gcttcatggt ggcggaggcg
     1801 gttggcggcg tgctgaccgg ttcgctggcg ctgctggcgg atgccgggca catgctggcc
     1861 gattcgattg cgctcggcct tgcctggtac gctttccacc tggccgaacg gccggcgacg
     1921 ggccgcctga cctatggttt cgggcgcgta aaaacgctgg tcgcctatac taacggcctt
     1981 gccatcttca tcatcgcgct gtggatcatc tatgaggcgt ggcagcgcct gcaagctccc
     2041 gcgccggtgc tcggcggccc gatgctggtg gtggcggggc ttgggctgat ggtcaatatt
     2101 gcgggctttc tcgtgcttca cggcggcaac cgcgccagcc tcaacatgcg cggcgccatt
     2161 ctgcatgtgc ttggcgacat gctgggttcg gccgccgcca tcgttgcggc ccttgtcata
     2221 ttggcgaccg ggtggacgcc catcgatccg atcctgtcgg tagtggtcgc gctgctcatc
     2281 ctttccaccg cctggtcgct gatgaaggag gcgggccatg tgctgctgga aggggtgccg
     2341 ccaagcctcg accgcgacgc ggtggcaagc gacctggtgg cgaatattcc gggcttgcgc
     2401 gaagtgcacc acatgcatgt ctggtccatc gacggcgaaa ccagcatggc gacgctgcat
     2461 gcctgcatgg caacggacgt cgatccctac cgggcggtcc tcgccatcaa gcaaaggctg
     2521 cgttcgcaac ataatatcag ccatgccacg gtcgagccgg aattcggccg ctgcacggac
     2581 ggcgaaggcg accatgacac gcaccacggg cacagccctg ggcacgaagc gtcgcactcc
     2641 gggcactatc attgaaggcc ggtgttgagt aagacccgtc cggtcagctt attccaactt
     2701 ccggaccggg atacgtcaat ggatcgcgac accagaaatg cggcagtcgc cgcgctcgtc
     2761 atcatggtgc tgttcggtgc ctttgcctat tacctgccgg tcatcatgct ggccgcaggc
     2821 aaggtctcgg gaattctcgc ggccatcgtg gctgcggtgt tcatgctggc gttgttcatg
     2881 gtgttctggc tgcgaagccg cagccagcgc ggcaagggcg actgacgggg ccgcctgccg
     2941 ggatttgcta agccgccagg ctggctgaca gcagcagaag tccaaacagg aacacgaacg
     3001 ccgccccacc gatttcgacg atcgaatgga tgcggttacc gatgcgcccg tctccggcga
     3061 ttgtcaccgc ccagttcttg gcggtaaccg caatgatcgc taaagccgaa acggtgatgg
     3121 cggtgccgag cgccatagcc agcaccgaca aaatgccacc cacccacagc ccgttcagca
     3181 gcgcgaagga cagtacgatc agcgcgccgg agcaggggcg gatgccgacg gcggccaccg
     3241 ctgtccaggc ggtgcgccag tcgaatcgtt cgccgccaag caaggcaggg tccggcgcgt
     3301 ggctatggcc gcagctttcg cagattgcga ttgcctgatg cttgtggccc tggtgctttt
     3361 gttcaccgtg agcgtgcgca tggtaattga gatggccggg ccgatggtcg cactgggaat
     3421 ggtcgccccg gaaatggccg ttatgggaat gggccgcggc ggccgacagg ctgtttgcct
     3481 cgcgcgaacc ggacatgcgc cgcagcgccg gcacggcctt gcgccacaaa agccacgcac
     3541 cgaaagcggt gacgaagaca tagcttgaga tttcgaggaa ccaggcggcg tcggtcatcg
     3601 acacggaggt gccgcgcagc acgaaatagg cgagcagcat gacgacgatg gcggtaatgg
     3661 cctgaagcag ggccgagatg aacgacagta gaatgccgcg tctcagcgcc acctcattgg
     3721 ccaccatata ggacgaaatc accgccttgc cgtgaccggg gccggcggca tggaaaatgc
     3781 cgtaggcgaa agacaggccg accagaagcc acagcttgct gccatcctgg cgcatcgcct
     3841 tcatggcatt ggccagcgcc ctgtagaaaa tctgctgctg ctggttgatc cacagcaatt
     3901 gatgcgcgaa caggccggtg cttgccggca tgccgtcatt ggtgccgatg cccagcgaac
     3961 tctgggcgtg cgcgaggctg gcgaaaagat tggcggcgac aaggatcgcc agcaggcaaa
     4021 gcacggagcg tagggcgttt cgggtcaccg gcttcatcct ttggcctggc aggtcagttc
     4081 cagcttggtg gcgaagatct tgctcatgtc ggtgccggtc gggtcgttga agaaggcatc
     4141 ggtcaacgtc ttctggttct gggcgattgc ggtgtcggga tcggggcgga tcacggtctt
     4201 ggcacagttc gccggcatgt cgtcgaccgt catgttggcg tcctcggtga aatcgatggc
     4261 ggtgtagaag gtcgggtcgt agacgccgaa atccaccttg cctgccagct tgatcggctt
     4321 ttccggttcg gattcgaaca ggatgatgag ctggtcgttg tcgaaattgg cgatcagatg
     4381 cgcaggggcc ttcatcttca cgtctttgcc gtccaccgtc acgagctgga aataatcata
     4441 ttcggccagc gaggcgaaga tcgtttcgga gacttccttc agctcggcat cgtccagctt
     4501 gaggtcggaa ttcttgtcga actccatcag caccgtgctc gaaaacaggt cgtcgaagcg
     4561 ccaaaggtgc cgcaaggcct tcaccgtttg acggtccggg ttgagcacca catcgagccg
     4621 ggcctcggcg aagacatgcg gatgcgcctg cgcggtatgg gcggctgcaa acagcgacac
     4681 cgctccggcg gccaatgttg gaattcgcct ttttaggttc attctgatcg cgaatccgtc
     4741 gcctgcctac gattgccgag gtttaccaga aacggggcga aattcggaca gcaatgcggc
     4801 tgcaacggtt atgccgcatc catgttcttg aaccattgcg ccaggaagtc cacgaaaacg
     4861 cgcactttca ccggcaggta gcggcggtgc gggtagacgg cgaagatgcc gccgcccggc
     4921 aggatacggt tgtccagcgc ggtaaccagc ttgccgcttt caatgtcggg cgcggcgatg
     4981 aagtccggca aaatggaaaa gccgagacca gccacggcgg cggcccttgc cgccatcggg
     5041 ctgttgactt ccagcgggcc ggacaccggc acgctgacgg cgtcgccatt gtcgcccttg
     5101 aaaggccagt tggcgagcca gcggccattg gtgtcgatga tgcagggaat gcgcgacaaa
     5161 tcctgcgggc gcgtcggcat gccgtgcttt tcgatgagtt cgggcgacgc gcaaagccgc
     5221 accgaaaacg gcgccaggcg tttggcgatc aacgaggagc tttccagcct ggaaatgcgg
     5281 atggccaggt cgaagccttc ctcgacgaga tcgacgaagc ggtcgtcgag gcgaacgtcg
     5341 aggacgattt cggggtattg ccttgcgaag tcgatcaac
//
LOCUS       DASYCS010000086         1664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4511109,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000086 DASYCS010000000
VERSION     DASYCS010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4511109"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1664
                     /locus_tag="VGM46_02990"
     CDS             <1..>1664
                     /locus_tag="VGM46_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006339256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HEY4191589.1"
                     /translation="DVTVADALDDFVLQAPDGPKLIGFLKTMEFSTLTRRVAEATGTE
                     AGAIEAAKVAVERADTAHGPDVGSGVQPGAGEGETAGAGKGQDTPSLLSALRAEKAVA
                     GKVDTASYLTIRDLPTLKAWVAEALEAGIFAFDTQTTTQDPMQAELCGFSLATRPGRA
                     AYVPLAHKSGEGDLLGGGLVENQIPVREALAAIKPLMADASVLKVMQNAKFDLVVMQR
                     HGLDIAPYDDTMLISYVLDAGTNAHNLESLSEKWLGHTAIAFKDVTGTGKATLAFDHV
                     AIERATAYAGEEADVALRLWHLLKPRLAAKGLVSVYERLERPLVRALALMEQRGISID
                     RQILSRLSGELAQRAAGLEEEVYQLAGETFPIGSPKQLGDILFGRMGLPGGAKTKTGQ
                     WSTTAQVLEELATEGYELPRKIVDWRQLTKLKSTYTDALPGYVHPETKRVHTSYALAA
                     TSTGRLSSVEPNLQNIPIRTTEGRKIRTAFIADKGNKLISADYSQIELRILAHMAEIP
                     QLVQAFSDGADIHAMTASEMFGVPVEGMPSDVRRRAKAINFGIIYG"
BASE COUNT          315 a          559 c          516 g          274 t
ORIGIN      
        1 cgatgtgacg gttgccgacg cgctcgacga tttcgtgctg caagcacccg acgggccgaa
       61 actgatcggc ttcctcaaga cgatggagtt ttccacgctt acccgccgtg tcgccgaagc
      121 gaccggcacc gaggccggcg caatcgaggc cgccaaggtg gcggtggagc gcgccgacac
      181 cgcgcacgga ccggatgtag gctcgggtgt gcagcctggc gccggagaag gcgaaacggc
      241 tggcgccggc aagggccagg acacgccctc ccttctttcc gcgctgcgcg ccgagaaagc
      301 cgtcgccggc aaggtcgaca ccgccagcta tttgacaatc cgcgatctgc cgaccctcaa
      361 ggcctgggta gcggaagcgc tggaggccgg tatcttcgcc ttcgacacgc agacaaccac
      421 gcaagacccg atgcaggccg aactgtgcgg cttttcgctg gccacccgcc ccggccgcgc
      481 cgcctatgtg ccgctggcgc acaagtcggg cgaaggcgat ctgcttggcg gcgggctggt
      541 ggaaaaccag atccccgtcc gcgaggcgct ggccgccatt aagccgctga tggccgacgc
      601 ctcggttttg aaggtcatgc agaacgccaa gttcgatctg gtggtgatgc aacggcacgg
      661 cctcgatatc gcgccttacg acgacaccat gttgatttcc tatgtgctcg acgccggcac
      721 caatgcgcat aatctggaat cgctgtctga aaaatggctc ggccacaccg caatcgcctt
      781 caaggacgtg accggcaccg gcaaggcgac gcttgccttc gaccacgtcg ccatcgagcg
      841 cgccacggcc tatgccggcg aggaagcgga cgtcgcgttg cgcctatggc atctgctgaa
      901 gccgcgcctt gccgccaagg gcctggtctc ggtctatgag cggctggagc ggccattggt
      961 gcgcgcgctg gccttgatgg aacagcgcgg catctcgatc gaccggcaaa tcctgtcgcg
     1021 gctgtcgggc gaactggcgc agcgcgcggc ggggctggag gaagaagtct accagcttgc
     1081 cggcgagacc tttcccatcg gctctcccaa gcagcttggc gacatattgt tcgggcgcat
     1141 gggcctgccc ggcggcgcca agaccaagac cggccaatgg tcgacgacgg cgcaggtgct
     1201 ggaggaactg gcgacggaag gctacgaact gccgcgcaag atcgtcgatt ggcgccagct
     1261 taccaagctg aaatcgacct acaccgacgc cctgcccggc tatgtgcatc ctgaaacgaa
     1321 gcgcgtccac acctcttatg cgctcgccgc cacctcgaca gggcggctgt cctcggtcga
     1381 acccaatctc caaaacatcc cgatccgcac caccgagggc cgtaagatcc gtaccgcctt
     1441 catcgccgac aaaggcaaca aattgatctc ggctgattac agccagattg agctgcgcat
     1501 cctcgcccac atggccgaaa ttccccagct cgttcaggcc ttttcggacg gcgccgacat
     1561 ccacgccatg accgcctcgg aaatgttcgg cgtgccggtc gaaggcatgc cttcggacgt
     1621 gcgccggcgc gccaaggcca tcaatttcgg catcatctac ggca
//
LOCUS       DASYCS010000087         9488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4222156,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000087 DASYCS010000000
VERSION     DASYCS010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 9488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9488
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4222156"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..1144)
                     /locus_tag="VGM46_02995"
     CDS             complement(101..1144)
                     /locus_tag="VGM46_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006206054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HEY4191590.1"
                     /translation="MRTGPFTAILFCAPALLAAAPAFCAEPPYLDDRSSAETVLKSLY
                     NAVSRHEYARAWDYFGETKPATDFDVFVKGFEGTDHVDVTTGGETSEGAAGSIYFTVP
                     VAIRAVGGDGTEKIFSGCYTLRQVNGQIQSPPFNPIHIEKGELKPATGAFEASVPASC
                     GDGPPPPKKDAVLEQAKAMFAAAYGSQCDPTLPGGEAVGVPEEHVIHYTAGGDSGEKA
                     ARLFRFFCSTGAYNENAVYYLSTATDTLRQLQFATPELDIRYENDDNQGKVEAVNIIG
                     FQTDERLVNSSYDDTTKSIVSHNRWRGVGDAGTSGTWLFRDGQFSLVQFDVDASYDGQ
                     SNPETVLDYNTAP"
     gene            1273..1779
                     /locus_tag="VGM46_03000"
     CDS             1273..1779
                     /locus_tag="VGM46_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008875518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 family protein"
                     /protein_id="HEY4191591.1"
                     /translation="MTISMYEASVPVFTARLKVLSKLLAIAENNAAERKIDEQVFMTA
                     RLAPDMLPLTKQVQIATDHAKGAPSRLAGREVPKYDDSEASFAELQARIEKTLAHLAT
                     FSAKDIDGSDDKTIELKLGGTEMSLAGMRYLLDVAMPNFYFHIVTAYDVLRHNGVPVG
                     KLDFLGGR"
     gene            complement(1838..2002)
                     /locus_tag="VGM46_03005"
     CDS             complement(1838..2002)
                     /locus_tag="VGM46_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191592.1"
                     /translation="MGFLTELFSRPRPQDQARYRSALALLHSMSNSDRADIGIKPADF
                     PRLAREMAMR"
     gene            2247..2708
                     /locus_tag="VGM46_03010"
     CDS             2247..2708
                     /locus_tag="VGM46_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006206058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191593.1"
                     /translation="MLDDLFVACEQPEETGAQPSIPFDYLSVADELHSGRIKVASGTL
                     DAEYRENRETAADLAAELAAFLDQAAPVEAEAKPVEKLPPIDPESIAAELGLAQPSTP
                     ADFGQIRRSFAFSNHPDRVAPHLRHRAVVRMQIANMLIDEARRKALAAARR"
     gene            2936..4198
                     /locus_tag="VGM46_03015"
     CDS             2936..4198
                     /locus_tag="VGM46_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006206060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HEY4191594.1"
                     /translation="MAFPIDNLRKEFPALGISDKGRRRIYLDNPAGTQVPQAVADAVS
                     RCLLTTNANLGGFFETSHAAQAVVADAHQAMADFLGAATPEEIIIGANMTTLTYHMSR
                     TLGRDFKAGDEIIVTRMDHEGNVAPWLQMAEDRGLVVHFLPFDEDSWQVEEAALSALL
                     SEKTRLVALNYASNLTGSINRVKALTALARKAGALVFVDAVQFAPHGLVDVQEIGCDF
                     LACSAYKFFGPHLGVLWGRRDVIDALKPYKCRCSSNGLPERFETGTPQIELLAGLSAA
                     IGHFEKLGEAAGERGSRRQKIAAAYAASVAYENPLAQRLIDGLADIAGLKIRGITDPR
                     RLHERVPTVSFTVEGIVPETIARQMNAENIFLWSGHNYAWEIVHQLGIPPEDGVVRIG
                     IAHYNTQGEIDETLESVHRTIAMLRQQR"
     gene            4274..4522
                     /locus_tag="VGM46_03020"
     CDS             4274..4522
                     /locus_tag="VGM46_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008875526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HEY4191595.1"
                     /translation="MLRVEKIKVGDIYVPTARRKTLHPETVRHLAEDILENGMKTPIQ
                     VRHDGKRHILVEGLHRLEAAKWLGETTIDAYLVQAKRH"
     gene            4559..4936
                     /gene="crcB"
                     /locus_tag="VGM46_03025"
     CDS             4559..4936
                     /gene="crcB"
                     /locus_tag="VGM46_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015317968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEY4191596.1"
                     /translation="MYNLVLVVIGGGLGAGARYLANIGAMRLVGPNFPWGTMAINIAG
                     SFAMGVFVALLGRRFGGSNELRMFIATGILGGFTTFSSFSLDFATLWERGTPLPAFGY
                     ALASVIGGIIALFVGLWLARTVS"
     gene            4975..5964
                     /locus_tag="VGM46_03030"
     CDS             4975..5964
                     /locus_tag="VGM46_03030"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018428051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEY4191597.1"
                     /translation="MAGVEQITVEAGEAGMRLDRWFKSHFPGLGFGHLQKLLRSGQVR
                     VDGGRVKADTRVEPGQQVRIPPMEVDKKGGGTEHLTGHSIRNQDDGDVLAKMLIHEDE
                     KVFVFNKPPGLAVQGGSGVTRNVDEMLEAWRNKKGEKPRLVHRLDRDTSGVLVVARTR
                     LAAMKLAEAFRARETEKTYWALVKGVPPKREDKISTWLIKEPTPDGDRVRIAKHGEKG
                     ADHAVSYYRIIEQAAQALTWLEMQPYTGRTHQLRVHAAHLGCPIIGDPKYFEADTNWD
                     FPGGMQNRLHLHARRIRIPHPDRGVIDVSAPMPQHMRQSWNLIGFDDASAEDS"
     gene            6074..6901
                     /locus_tag="VGM46_03035"
     CDS             6074..6901
                     /locus_tag="VGM46_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HEY4191598.1"
                     /translation="MNKKLVRKAVIALLVLAVAFYFIPIITTIWVVCGLIDVMRNSKK
                     SWSLFVGYFAGNGVFTWLLSPFNLLVDLISYRNPGVWKLDDFPADYRAEIETVLDTFK
                     QRQEDIMADIDAVFPSGRRGMYVYQWYGKQHIDSVPEFNKHYKYVRTIAVSVFSGRES
                     TSFHFGPLRLTLRVLYNLKPAITDNIFIECGNTRNLWYKNPLFIFDDTLLHRSVNEHD
                     GRRYCVFMDIIRPSPLPGVLSAALAVISTGVDRINAVFYKNWKVIGKAKPAAGPSAT"
     gene            6917..7708
                     /locus_tag="VGM46_03040"
     CDS             6917..7708
                     /locus_tag="VGM46_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP12 family chaperone protein"
                     /protein_id="HEY4191599.1"
                     /translation="MRDILNDLEAGKYLSDPDPVRRAQIQMKTHLPKRFYKEASVAAA
                     EGGFAVHLDGKPVRTPAKALLTLPTEQSARLVADEFAAQVEVIDPVKMPVLRLVNTAL
                     DGVANEKQAVLEDILRFASTDMLCYRADTPQGLVDRQNTHWDPVLDWARAALGARFNL
                     AEGVIHVEQPRESIAVLGAHLARRADPLRLAALHLMTSLTGSALLALAADEGELDAEA
                     AWLAAHVDEDWQIEQWGQDAEAVARRNARHRDFLAALQLLEALNP"
     gene            complement(7761..9170)
                     /gene="glnA"
                     /locus_tag="VGM46_03045"
     CDS             complement(7761..9170)
                     /gene="glnA"
                     /locus_tag="VGM46_03045"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018094964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEY4191600.1"
                     /translation="MTTAADIMKQIQENDVKFVDLRFTDPKGKLHHVTMDVGEIEEDT
                     FADGVMFDGSSIHGWKAINESDMVLMPDTESAHMDPFFAQSTMVILCDILDPISGEAY
                     NRDPRGIAKKAEAYMKAEGIGDTIYIGPEAEFFIFDDVKYKADPYNTGFKLDSSELPS
                     NDDTDYETGNLGHRPRVKGGYFPVPPIDSCQDMRSEMLTVMQEMGVHVEKHHHEVAAA
                     QHELGIKFDTLVRNADKILIYKYVVHQVANAYGKTATFMPKPIFGDNGSGMHVNMSIW
                     KGGKPMFAGNEYAGLSETCLFFIGGVIKHAKAINAFTNPLTNSYKRLVPGYEAPVLLA
                     YSARNRSASCRIPFGTSPKAKRLEVRFPDPGANPYLAFSALLMAGLDGIKNKIHPGQP
                     MDKDLYDLPPKELKKIPTVCGSLREALQSLDKDRGFLKAGGVFDDDQIDSYIELKMAE
                     VMRFEMTPHPVEFDMYYSV"
     gene            complement(9217..>9488)
                     /locus_tag="VGM46_03050"
     CDS             complement(9217..>9488)
                     /locus_tag="VGM46_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018900556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEY4191601.1"
                     /translation="GLQGITVTEAKGFGRQKGHTELYRGAEYVVDFLPKVKIELVLGD
                     DAVEAAVEAIRKAAQTGRIGDGKIFVSNIEEVVRIRTGETGIDAV"
BASE COUNT         1786 a         2906 c         2901 g         1895 t
ORIGIN      
        1 aacaaagagc atttccagca aaagtgtgaa acggttttgc gtccggaaat gcggcaaaaa
       61 acaaagagat agagcggttt accgattcgg taatatcgtt ctaaggagcg gtgttgtagt
      121 cgagcacggt ttcgggattg ctctgcccgt cataggaagc atcgacgtcg aactgcacca
      181 gcgagaactg cccgtcgcga aacagccatg ttcccgatgt gccggcgtcg ccgacgccgc
      241 gccatctatt atgtgaaacg atcgatttgg tggtgtcgtc atagcttgaa ttgaccagcc
      301 gctcgtcggt ctggaagccg atgatgttga cggcctcgac cttgccctga ttgtcgtcgt
      361 tttcgtagcg gatatccagt tccggcgtgg cgaactgtag ctgccgaagc gtgtccgtgg
      421 cggtggacag gtagtagacc gcgttttcat tgtaggcgcc cgtcgaacag aagaagcgaa
      481 acagtcgcgc tgccttttcg ccgctgtcgc cgccggcggt atagtggatg acatgttctt
      541 ccggcacgcc gaccgcctca ccgccgggca gcgtcgggtc gcactggctg ccataagcgg
      601 ctgcaaacat cgccttggcc tgttccagca ccgcatcctt cttcggcggc ggcgggccgt
      661 cgccgcagct tgccggcacg gaagcctcga aggcgccggt cgccggcttc aactcgccct
      721 tttcgatatg gatcgggttg aagggcgggc tctgaatttg gccgttgacc tgccgcagcg
      781 tgtagcagcc ggaaaatatc ttctcggtcc catcgccgcc aacggcgcgg atggcgacag
      841 gcacggtgaa gtaaatgctg ccggcggccc cctcgctggt ctcgccgccg gttgtcacgt
      901 cgacatggtc ggtgccctcg aagcccttga cgaagacatc gaaatccgtg gcgggcttcg
      961 tctcgccgaa atagtcccag gcgcgggcat attcatgccg gctgaccgcg ttgtagagcg
     1021 atttcagcac ggtttcggcg ctcgacctgt cgtccagata gggtggttcg gcgcaaaatg
     1081 ctggcgcggc cgccagcaat gccggcgcac agaacaatat tgcggtgaag ggaccggttc
     1141 tcatcgcgaa tctccgctca acagagagtg atcgtaacgc ggaatgcggc gcgcccatga
     1201 cgcttgaaga atcgtgcgcg aaggcgctac gtcgcgccgg cggcaattcc agccgcgcca
     1261 ggagaaattg acgtgaccat ttccatgtac gaggcatccg tgcctgtctt tacagcgcgt
     1321 ttgaaggtgt tgtccaagct gctggcgatt gccgaaaaca acgccgccga acgcaagatc
     1381 gacgagcagg tgttcatgac cgcccgcctt gcgccggaca tgctgccgct gaccaagcag
     1441 gtgcagatcg ccaccgacca cgccaagggc gcgccgtcgc ggctggccgg gcgcgaagtg
     1501 cccaaatacg acgatagcga ggcgagcttt gccgagcttc aggcccgcat cgaaaagacg
     1561 cttgcccatc tggcgacgtt ttcggcgaag gatatagacg gctccgacga caagacgatc
     1621 gaactcaagc ttggcggcac cgaaatgtcg ctggccggca tgcgctacct tctcgacgtc
     1681 gccatgccga acttctattt ccacatcgtc accgcttacg acgtccttcg ccacaatggc
     1741 gtgccggttg gcaagctgga ctttctcggc ggtcgctgag cgcaacagcg acaagaagct
     1801 ccccggcaat gccggggagc ttcggttgat gaatggctta acgcatcgcc atttcgcggg
     1861 cgaggcgggg aaaatcggcg ggcttgatgc cgatatcggc ccggtccgaa ttgctcatcg
     1921 agtggagcag cgcaagcgcc gaacggtagc gcgcctgatc ctgcgggcgc ggacgggaaa
     1981 acagttctgt caagaacccc attttcaggc tcctttgttg gtcctcccaa gacctgcata
     2041 taggtgaatc gtgacaattg tgcgctgcaa cattcgcatg gcagccatgc gatgcgaaat
     2101 tttgcaattg cctcaattcc cttcacctta tggcatttat ttttaacgca cccattttag
     2161 gtttgtggcc gcgtatcggc ggtgccgtgc gctgaaacag ccaggaggcc aaaatcgccg
     2221 ggggcagaac caaggatttc gcggcggtgc tcgacgatct tttcgtcgct tgcgagcagc
     2281 cggaggaaac cggcgcccag ccgtccattc cgttcgatta tctgtcggtc gccgacgaac
     2341 tgcattcggg ccgcataaag gtcgccagcg gcacgctgga tgccgaatac cgtgaaaatc
     2401 gcgaaaccgc cgccgatctg gccgccgagc ttgcagcctt tctggaccag gccgcaccgg
     2461 tcgaggccga ggcaaagccc gtcgaaaaac tgccgccgat cgatcccgaa tccatcgccg
     2521 ccgaactcgg gcttgcccag ccttcgacgc cggcggattt tggccaaatc cgccgcagct
     2581 ttgccttttc caaccatccc gaccgggtcg cgccgcattt gcgccaccgc gccgtggtgc
     2641 gcatgcagat cgccaacatg ctgatcgacg aggcgaggcg caaggcgctg gccgccgcgc
     2701 ggcgctagag cggttctgtt tttaccgaat cggtaaaacc gctctatctc tttgttttgg
     2761 ccgcatttcc ggacgcaaaa ccgtttcatg cttttgctgg aaatgcgctg aaaaatttcc
     2821 tgccataata caagtttgcc gctgcccctg cctgccgggg tggcgtttcc ctttgcgatg
     2881 gttgaagaat tggctgccgt ccgaggcacg ggcaattcat cttcaagggg cattcatggc
     2941 atttcccatc gacaatctgc gcaaggaatt tcccgcgctc ggcatcagcg acaaaggccg
     3001 ccgccgcatc tatctggata atccggccgg cacgcaggtg ccgcaggcgg tcgccgacgc
     3061 cgtttcgcgt tgcctgctga ccaccaacgc caatctgggc gggttcttcg agacgagcca
     3121 tgccgcgcag gctgtcgttg ccgacgccca tcaggcgatg gccgatttcc ttggcgcagc
     3181 gacgccggag gaaatcatca tcggcgccaa catgacgacg ctgacctacc acatgtcgcg
     3241 cacgcttggc cgggacttca aggccggcga cgagatcatc gtcacccgca tggatcatga
     3301 aggcaatgtc gcgccatggc tgcaaatggc cgaggacagg ggcctcgtcg tgcacttcct
     3361 gccgttcgac gaggatagct ggcaggtaga ggaggcggcg ctctcggcgt tgctcagcga
     3421 aaagacgcgg ctggtggcgc tgaattatgc cagcaacctg accggctcga tcaaccgcgt
     3481 gaaggcgctg acggcgcttg ccaggaaagc cggcgcactg gtgttcgtgg atgccgtcca
     3541 gttcgcgccg cacgggctgg tcgacgtgca ggagataggc tgcgatttcc tggcctgctc
     3601 ggcctacaaa ttcttcgggc cgcatctggg cgtcctgtgg ggcaggcgcg acgtcatcga
     3661 cgcgctgaag ccatataaat gccgctgctc gtccaacggc ctgccggagc gtttcgaaac
     3721 cggcaccccg cagatcgaat tgctggccgg cctgtcggcg gcaatcggcc atttcgaaaa
     3781 gcttggcgag gcggcgggcg agcgcggctc gcgccgccag aagatcgcgg ccgcctatgc
     3841 cgcctccgtc gcctatgaaa atccgctggc gcagcgcctc atcgacgggc ttgccgatat
     3901 tgcgggcctt aaaatccggg gtatcaccga ccccaggcgc ctgcatgagc gggtgccgac
     3961 cgtttccttc actgtcgaag gcatcgtgcc ggaaaccatc gcgcggcaga tgaatgcgga
     4021 gaacatcttc ctgtggtccg ggcataatta tgcgtgggaa atcgtccacc agctcggcat
     4081 cccgccggaa gacggcgtcg tgcgcatcgg catcgcccac tacaacacgc aaggcgagat
     4141 cgacgagacg ctggaaagcg ttcatcgcac catcgctatg ctgcgccagc agcgctagct
     4201 ggctctatat gtttggcctg cccggcaaaa tgctctatcc cggtggaata gcgtctcgtt
     4261 tgccggagca gccatgctga gagtggaaaa gatcaaggtc ggggatatct acgtgccgac
     4321 ggcgcgcagg aagaccttgc atcccgaaac ggtgcggcat ctggccgagg acatcctgga
     4381 aaacggcatg aagacgccga tccaggttcg ccacgacggc aagcgccata tactggtcga
     4441 aggcctgcac cggctggagg cggcgaaatg gctgggcgaa accaccatcg acgcctatct
     4501 ggtgcaggcg aagcggcatt gatgtttttt ccgcacaggg gctgagcagg tggtcggcat
     4561 gtacaatctt gttctcgtgg tcatcggcgg cggactgggc gccggtgcgc gctatctggc
     4621 caatatcggc gcgatgcgcc ttgtcggccc gaattttccg tggggcacga tggccatcaa
     4681 catcgccggc tccttcgcta tgggcgtgtt cgtggcgctg cttggccggc gctttggcgg
     4741 ctccaacgaa ctgcgcatgt tcatcgctac cggcatattg ggcggcttca ccaccttctc
     4801 gtcgttctcg ctcgatttcg ccacgctttg ggagcgcggc acgccgcttc cggcgttcgg
     4861 ctatgcgttg gccagcgtaa ttggcggcat cattgcgctg tttgtcggac tgtggctcgc
     4921 aagaaccgtt tcttagtgct atctccgcgc ttcgaaacgg atagatgaag aacaatggca
     4981 ggcgttgaac agatcacggt ggaagccggc gaggcgggga tgcggctcga ccgctggttc
     5041 aagagccatt ttccgggcct tggcttcggc catttgcaaa agcttttgcg ctccggccag
     5101 gttcgcgtcg atggcggccg cgtcaaggcc gacacgcgcg tggaacccgg ccagcaggtg
     5161 cgcattccgc cgatggaagt ggacaagaag ggcggcggca ccgaacactt gaccggccat
     5221 tcgatccgca accaggacga tggcgacgtt ctggccaaga tgctgatcca tgaggacgag
     5281 aaagtgttcg tcttcaacaa gccgccgggg ctggccgtgc agggcggttc gggcgttacc
     5341 cgcaatgtcg acgaaatgct ggaagcctgg cgcaacaaga agggcgagaa gccgcgcctc
     5401 gtccaccggc tcgaccgcga cacgtcgggc gtgctcgtcg ttgcccgcac gcgccttgcc
     5461 gccatgaagc ttgccgaggc gttccgggcg cgcgaaacgg aaaagaccta ttgggcgctg
     5521 gtaaagggcg tgccgcccaa gcgcgaggac aagatttcaa cctggctcat caaggagccg
     5581 acgccggacg gcgaccgcgt gcgcatcgcc aagcatggcg aaaagggcgc cgaccacgcc
     5641 gtctcctact atcgcatcat cgagcaggcg gcgcaggcgc tgacctggct ggaaatgcag
     5701 ccctatacgg gccgcaccca tcagcttcgc gttcatgccg cccatctcgg ctgtccgatc
     5761 atcggcgatc cgaaatattt cgaggccgac accaactggg actttccggg cggcatgcag
     5821 aaccggctcc atcttcacgc ccgccgcatc cgcattcccc accccgacag gggcgtcatc
     5881 gacgtttccg cgccgatgcc gcaacacatg cgccagagtt ggaacctgat tggcttcgac
     5941 gatgccagcg ccgaggattc ctgacgagcg cccacttcaa acataattta ccgcgtggcc
     6001 gccatttgtc ggtctatatc gcagtcgata atcggccagg gccgaccccg ttcaccacac
     6061 cgtggatttt gtcatgaaca agaaactagt ccgcaaagct gtgatagcgc tgctcgtttt
     6121 ggcagtagcg ttctatttta ttcccatcat cacgaccatc tgggtcgtct gcggcctgat
     6181 cgacgtgatg cgcaacagca agaaaagctg gagcctcttc gtcggctatt tcgccggcaa
     6241 cggggtgttc acctggctgc tttcgccgtt caacctcctg gtcgacctga tttcctaccg
     6301 caatcccggc gtctggaagc tcgatgattt ccccgccgac tatcgcgccg agatcgagac
     6361 ggtgctcgac accttcaagc agcggcagga agatatcatg gccgatatcg acgcggtgtt
     6421 ccccagcggg cggcgcggca tgtatgtcta ccaatggtat ggcaagcagc acatcgacag
     6481 cgtgccggag ttcaacaagc actataaata tgtccgcacc atcgccgtct cggtattcag
     6541 cggccgcgaa tcgacctcgt tccatttcgg tccgctgcgg ctgacgctgc gcgtactcta
     6601 caacctcaag ccggccatca ccgacaatat tttcatcgag tgcggcaaca ccaggaacct
     6661 gtggtataaa aacccgctct tcatcttcga cgatacgctg ctccaccgtt cggtcaatga
     6721 acatgatgga cgccgctatt gcgtgttcat ggatattatc cgcccatccc cgctgccggg
     6781 tgtgctgtcg gcggcgcttg ccgtcatttc cacgggcgtc gatcgcatca acgccgtgtt
     6841 ctacaagaac tggaaggtca tcggcaaagc caagcctgcc gccggcccat ccgccacctg
     6901 aaaaccgggc catttgatgc gcgatatact gaacgacctc gaagccggga aatacctttc
     6961 cgacccggac ccggtgcgcc gcgcccaaat ccagatgaaa acgcacctgc ccaagcgttt
     7021 ctacaaggag gcttccgtcg cggctgccga gggcggcttc gccgtccacc tggacggcaa
     7081 gccggtgcgc acgcccgcca aggcgctgtt gacgctgccc acggagcagt cggcgcgcct
     7141 cgttgccgat gagttcgccg cgcaagtcga ggtcatcgat ccggtgaaaa tgccggttct
     7201 gcggctggtc aacacggcgc tcgacggtgt cgccaacgag aagcaggcgg tgctggaaga
     7261 tattttgcgc ttcgcctcga ccgacatgct ttgctaccgc gccgacacgc cgcaagggct
     7321 ggtggaccgc cagaacacgc attgggaccc ggtgctggac tgggcaaggg cggcgcttgg
     7381 tgcgcgcttc aacctcgcgg agggggtcat ccatgtcgag cagccgcgcg aatccatcgc
     7441 ggtgctgggc gcccatctcg cccgccgcgc cgatccgctc aggctggcgg cacttcacct
     7501 gatgacctcg ctgaccggct cggcgctgct ggcgctggcc gccgatgagg gcgaactgga
     7561 cgcggaggcc gcctggcttg ccgcccatgt cgatgaggac tggcagatcg aacaatgggg
     7621 ccaggatgcc gaggcggtcg cgcgccgcaa cgcccgccac cgcgatttcc tcgccgcctt
     7681 gcaattgctg gaagcgctta acccataaaa aaaccccggc gtttgcgccg gggttttttc
     7741 gttgggagcg ataaattcgg tcagaccgaa taatacatgt cgaattcgac cggatgcggg
     7801 gtcatttcga agcgcatcac ttccgccatc ttcaactcga tatagctgtc gatctggtcg
     7861 tcgtcgaaca cgccgccggc cttcaggaag ccgcggtcct tgtcgaggct ttgcagcgct
     7921 tcgcgcagcg aaccgcacac ggtcgggatc ttcttcagtt ccttcggcgg caggtcgtag
     7981 agatccttgt ccatcggctg gccggggtgg atcttgttct tgatgccgtc gagaccggcc
     8041 atcaacagcg ccgagaaggc gaggtagggg ttcgcgcccg gatcggggaa gcgtacttca
     8101 aggcgcttcg ccttgggcga ggtgccgaac gggatgcggc aggaggccga gcggttgcgc
     8161 gccgaatagg ccagcagcac cggcgcttca tagcccggaa ccagccgctt gtaggagttt
     8221 gtcagcgggt tggtgaaggc gttgatcgcc ttggcgtgct tgatgacgcc gccgatgaag
     8281 aacaggcagg tttccgacag gccggcatat tcattgccgg cgaacatcgg cttgccgccc
     8341 ttccagatcg acatgttgac atgcatgccc gagccgttgt cgccaaaaat cggcttcggc
     8401 atgaaggtgg ccgtcttgcc ataggcgttg gcgacctggt gcacgacata tttgtagatc
     8461 aggatcttgt cggcgttgcg caccagcgtg tcgaacttga tgccgagttc gtgctgggcc
     8521 gccgccacct catggtggtg cttttcgaca tgcacgccca tttcctgcat gacggtcagc
     8581 atttccgagc gcatatcttg gcacgaatcg atcggcggca ccgggaaata gccgcccttg
     8641 acgcgcggac ggtggccgag attgccggtc tcgtagtcgg tgtcgtcgtt ggacggcagt
     8701 tccgacgaat ccagcttgaa gcccgtattg taggggtcgg ccttgtactt cacgtcgtcg
     8761 aagatgaaga attcggcttc cggcccgata tagatggtgt cgccgatacc ttcggccttc
     8821 atgtaggctt ccgccttctt ggcgatgccg cgcgggtcgc ggttataggc ctcgcccgag
     8881 atcggatcga gaatgtcgca caggatgacc atggtcgact gcgcgaagaa cgggtccata
     8941 tgcgccgatt ccgtatcggg catcagcacc atgtcgctct cgttgatggc cttccagcca
     9001 tggatcgagg agccgtcgaa catcacgccg tcagcgaatg tgtcttcctc gatttcgccc
     9061 acgtccatgg tgacgtgatg cagcttgccc ttggggtcgg taaagcgcag atcgacgaac
     9121 ttcacgtcgt tctcctggat ctgcttcatg atgtcggcgg cagtcgtcat gtcttggttt
     9181 cctgtgatga ttttttgaaa cttgtttggc ggcggctcaa acggcgtcga tgccggtttc
     9241 gccggtgcgg atgcgcacga cttcctcgat gttggagacg aaaatcttgc cgtcgccgat
     9301 gcgcccggtc tgcgccgcct tgcggatcgc ttcgaccgcg gcttccacgg cgtcgtcgcc
     9361 cagcaccagt tcgatcttca ccttcggcag gaagtcgacg acatattcgg cgccgcgata
     9421 aagctcggta tgacccttct ggcgtccgaa acccttggcc tcggtaacgg tgatgccttg
     9481 cagcccgg
//
LOCUS       DASYCS010000088         5042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1902301,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000088 DASYCS010000000
VERSION     DASYCS010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 5042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5042
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1902301"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="VGM46_03055"
     CDS             complement(<1..796)
                     /locus_tag="VGM46_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HEY4191602.1"
                     /translation="MAKALMLQGTGSDVGKTVLVAGLCRAARNRGLKVRPFKPQNMSN
                     NAAVADIPGEAGQSGEIGRGQWLQALACGVRPTVHMNPVLLKPQTDIGSQIIVQGKVF
                     GEAKARDYQGLKPRLMEAVLHSWAQVGEGADLVIVEGAGSPAEINLRAGDIANMGFAT
                     CANVPVVLVGDIDRGGVIASVAGTHLILPESDRHMVAGYLINKFRGDVSLFDEGIAAI
                     GEFTGWRCFGVVPWLKAAARLPSEDSVVLERLASGGGRALKVAVPML"
     gene            complement(796..1413)
                     /locus_tag="VGM46_03060"
     CDS             complement(796..1413)
                     /locus_tag="VGM46_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019858666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047869 - dimethylpropiothetin
                     dethiomethylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfonioproprionate lyase family
                     protein"
                     /protein_id="HEY4191603.1"
                     /translation="MGTIDDQLLAAFRSFLTALDSAVVRDVAGGIDWNMAQRPLEASK
                     LACLRHLDRATELASGDSLPVARLLADHRDEFHWGQTYGEADFGRHFIENYGWLELFG
                     TRGLFVNETMAGGFLILGPEIVYPDHHHVAEEIYVPLTGGTEWRMGEGGFVARRAGEV
                     IHHASNVNHAMRTGSEPLLALYIWRGGPLAQKSTVTGTVAQQGRA"
     gene            1547..2713
                     /locus_tag="VGM46_03065"
     CDS             1547..2713
                     /locus_tag="VGM46_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isobutyryl-CoA dehydrogenase"
                     /protein_id="HEY4191604.1"
                     /translation="MDAVVDATAGQFELNEDQRAIQEVARGFAADRVAPSALEWDRNR
                     HFPADVIRETGPLGLGGIYVRDDVGGSALSRLDAVLVFEALASACPAFSSFISIHNMA
                     ASMIDRFGNEAQRQRFLPRLTSMEWLASYCLTEPGSGSDAAALKTRAVRSDDGYVLNG
                     TKQFISGAGDSDLYVVMARTGADGPKGISAIVVPKDAPGLSFGAVEHKMGWHMQSTRQ
                     VVFEDCAVPAENLLSAEGAGFSIAMAGLDGGRLNIAACSLGGAQSALDKALAYSGERK
                     AFGKTIDQFQALQFKLADMETELQAARIFLYAAASKLDRKAPDAAKWSAMAKRLVTDT
                     GFEVANNALQLFGGYGYLHDYGVEKLVRDLRVHQILEGTNEIMRVIIARHLISR"
     gene            2733..3617
                     /gene="mmsB"
                     /locus_tag="VGM46_03070"
     CDS             2733..3617
                     /gene="mmsB"
                     /locus_tag="VGM46_03070"
                     /EC_number="1.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008442 - 3-hydroxyisobutyrate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyisobutyrate dehydrogenase"
                     /protein_id="HEY4191605.1"
                     /translation="MTTIAFIGLGNMGNPMAANLVKAGHTVHGFDLMPENLATARENG
                     VTVMASAAAVVKEADAVITMLPAGKHVLSVYGEIAPEAKSGALFIDCSTIDVESAKTA
                     HAMARKAGMLSVDAPVSGGTGGASAGTLTFMAGGADEAFAIAEPILKSMAGRIVYCGG
                     DGAGQSAKICNNMILGISMIGVAEAFVLAEKLGLSHQALFDVASTSSGQCWSLTTYCP
                     VPGPVPASPANRDYRPGFAAGLMLKDLKLSQEAAQSAGAVTPLGAEAAQLYALFNAQG
                     NAGSDFSGIINFLRGSTS"
     gene            4090..4311
                     /locus_tag="VGM46_03075"
     CDS             4090..4311
                     /locus_tag="VGM46_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191606.1"
                     /translation="MRKSTRIFILTAVIEALLIVGAVYMITQVTSGAWKTFDQADALD
                     RIYTVMGAAIGGIGGAFLFLALVLRLKGQ"
     gene            complement(4379..>5042)
                     /locus_tag="VGM46_03080"
     CDS             complement(4379..>5042)
                     /locus_tag="VGM46_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006335487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY4191607.1"
                     /translation="PMSYWPEVQRICRKYDVLLMLDEVITGYGRTGEWFAAQSFGIEP
                     DTITTAKALTSGYQPLSALLVGDRIAATLVEKGGEFNHGYTYSGHPVACAVALKNLEI
                     IEKEGLVERVRTDTGPYFAKTLQERLGGHALVGEVRAFGLMGAIEIVKDKAAKERFLP
                     AGSAAVTVRDHAIAQGLMLRATGDTMILSPPLIWTRATIDMACDRIARALDFAEADLR
                     RG"
BASE COUNT          951 a         1656 c         1503 g          932 t
ORIGIN      
        1 ccaacatcgg caccgccact ttcagcgccc gcccgccccc ggatgccaac cgctccagca
       61 ccaccgaatc ctccgagggc agccgcgccg ccgccttcag ccacggcacg acgccgaagc
      121 agcgccagcc ggtaaattcg ccgattgccg cgattccctc gtcgaacagg gaaacgtcgc
      181 cgcgaaactt gttgatgaga tagcccgcca ccatgtgccg gtcgctttcc ggcaagatca
      241 ggtgcgtgcc ggcgacggag gcgataaccc caccacggtc gatgtcgccc accagcacca
      301 cgggcacatt cgcgcacgtc gcaaagccca tattggcgat atcgccggct cgaaggttga
      361 tttccgccgg cgagccggcg ccctcgacga tgacgaggtc cgcaccctcg cccacctgcg
      421 cccacgaatg cagcaccgct tccatcagcc ggggcttcag gccctggtaa tcacgcgcct
      481 tcgcctcacc gaacaccttg ccctgcacga tgatctgcga gccgatgtcg gtctgcggct
      541 tcagcagcac cgggttcatg tgaacggtag gccgcacgcc gcaggccagc gcctgtagcc
      601 attgcccgcg cccgatctcg ccggattgcc cggcttcgcc cggaatatcg gcgacggcgg
      661 cattgttgga catgttctgc ggcttgaatg gccgcacctt aaggccccga ttcctcgccg
      721 cgcggcaaag cccggcaacc agcaccgtct tgccgacatc cgaaccggtg ccttgcagca
      781 tgagcgcctt ggccatcagg ccctgccctg ctgcgccacg gtgccggtaa cggtcgattt
      841 ctgcgccagc ggcccgccgc gccagatgta gagcgccagc agcggctccg aacccgtccg
      901 catcgcatgg ttgacgttgg aggcgtgatg gatgacctca ccggcccttc gcgcaacaaa
      961 gccgccctcg cccatgcgcc attccgtgcc accggtgagt ggaacgtaga tttcctcggc
     1021 gacatggtgg tggtcgggat aaacgatttc cggcccaaga atgagaaacc cgccggccat
     1081 ggtttcgttg acgaaaagcc cacgcgtgcc gaacaattcg agccagccgt aattttcgat
     1141 gaaatgcctg ccgaaatcgg cctcgccata ggtctggccc cagtggaatt cgtcgcgatg
     1201 gtcggccaac agccgcgcca ccggcaaact atcgccggag gcaagctcgg tggctcgatc
     1261 aagatggcgc aggcatgcaa gcttgctcgc ctccagcggc cgctgcgcca tgttccagtc
     1321 aatgcccccg gcaacatcgc gcacgacggc gctatccagc gccgtaagaa aggaacggaa
     1381 agccgccagc aattgatcgt caatcgttcc cacatccagc tccgttaaac accgtgcacg
     1441 ccacgtctat cagggttgcg cggcggcatc attggtctag attggctggc acgcatagcc
     1501 aaaaacgaca ggctggcgac aagtcgcaaa catcagcgag gacgccatgg acgccgtagt
     1561 cgatgcgacc gccggccaat tcgaactcaa cgaggaccag cgcgccatac aggaagtggc
     1621 gcggggcttc gccgccgacc gtgttgcgcc cagcgcgctg gaatgggacc gcaaccggca
     1681 tttcccggcg gacgttattc gcgagaccgg cccgctcggc cttggcggca tctatgtgcg
     1741 cgacgatgtc ggcggctcgg cgctttcgcg gctggatgcc gtgctggtat ttgaagcttt
     1801 ggccagcgcc tgcccggcct tttcgtcctt catttcgatc cacaacatgg ccgcctcgat
     1861 gatcgaccgt ttcggcaacg aggcgcaacg ccagcgcttc ttgcccaggc tgacctcgat
     1921 ggaatggctg gcaagctatt gcctgaccga gcctggttcc ggctcggatg cggctgcgct
     1981 caagacgcgg gcggtcagga gcgacgacgg ctatgtgctg aacggcacca agcagttcat
     2041 ttcgggcgcg ggcgacagcg acctctatgt cgtgatggcg cgcaccggcg cggacgggcc
     2101 gaaaggcatt tccgccattg tcgtgccgaa ggacgcgccg gggctttcct ttggcgcggt
     2161 ggaacacaag atgggctggc acatgcagtc cacgcgccag gtcgtcttcg aagattgcgc
     2221 ggtgccggcc gaaaacctgc tttcggccga aggcgccggc ttttccatcg ccatggccgg
     2281 gctggacggc ggcaggttga acattgccgc ctgctcgctg ggcggcgcgc agtccgccct
     2341 cgacaaggcg cttgcctata gcggcgagcg caaggcattc ggcaagacca tcgatcagtt
     2401 ccaggcgctg caattcaagc tggccgacat ggaaacggaa ttgcaggcgg cgcgcatctt
     2461 cctctacgcc gccgcctcca agctggaccg caaggcgccg gatgccgcga aatggtccgc
     2521 catggccaag cgtctcgtca ccgataccgg ctttgaagtc gccaacaatg cgctgcaact
     2581 gttcggcggc tatggttatc tgcacgacta cggcgtcgag aaactcgtgc gcgacctgcg
     2641 cgtccaccag atcctcgaag gcaccaacga gatcatgcgg gtgattatcg cccgccatct
     2701 catcagccgt tgaatttttc cgggagaatt gaatgactac catcgccttc atcggcctcg
     2761 gcaacatggg taatccgatg gccgccaatc tcgtcaaggc cggccacacg gttcacggct
     2821 tcgacctgat gccggaaaac ctggccaccg cgcgcgaaaa cggcgtcacc gtcatggcca
     2881 gcgccgccgc agttgtgaag gaggccgatg ccgtcataac aatgctgccc gccggcaaac
     2941 atgtgctgtc cgtctatggc gaaatcgccc cggaggcaaa gtccggagcg ctgttcatcg
     3001 attgctcgac catcgatgtt gaatcggcaa aaacggcgca tgcgatggcc cggaaggcgg
     3061 gaatgctttc cgtcgatgct cccgtctcgg gcggaaccgg cggcgccagc gccggcacgc
     3121 tgactttcat ggcgggcgga gcggacgagg ctttcgcgat tgcggaaccg atcctcaagt
     3181 cgatggcggg gcgcatcgtg tattgcggcg gcgatggtgc gggccagtcg gccaagattt
     3241 gcaacaacat gatcctcggc atctccatga tcggtgtggc ggaggccttc gttttggccg
     3301 aaaagctcgg cctttcgcac caggccctgt tcgatgtcgc ctcgacctcg tctggccaat
     3361 gctggtcgtt gaccacctac tgccccgtcc ccggcccggt tcccgcatcg cccgcaaacc
     3421 gcgactatag gccgggcttt gccgccggcc tgatgctgaa ggatctgaag cttagccagg
     3481 aagccgccca aagcgccggc gcggtgacgc ctttgggggc cgaagcagcg cagctttatg
     3541 cgctgttcaa cgcgcagggc aacgccggct cggatttttc cggcattatc aattttctgc
     3601 gcggaagcac cagctaacga aattctggaa aaaccgcggc cggttttcgt ccgcagcggc
     3661 gcaagacagg ggtttcgagc ggctaacaat gccgcagaac tccgaaccgg attttttgca
     3721 aacggcgaaa aagattacaa atttaggttt gcttaagctc tcgctaacca cgcggtaacg
     3781 atgtgcctgt agaacggatt tcgaggcgga catagcgcac cgcccaccgc tccggatcag
     3841 gttctgcgta ccggagcagt taagtttcgc gtgtatcgag tagcgaaacg ccatgcgtat
     3901 ctggcaaagc cgcgcctcct tcaggagcgc ggtttttgcg ttcagcccaa gcggttttgc
     3961 attaagccca gcaggtttgc attaaatcaa ggccagacaa tcttcgcaaa ttcctctgct
     4021 ggaccgcccg cttttcatcg cctatgcatc gtcttccaac ctaggcttgt ccggaaaggc
     4081 ggcgcaacca tgagaaaatc cactcgcatc ttcatcttga ccgccgtaat cgaggcgctg
     4141 ctgattgtcg gcgccgtcta catgattacc caggtgactt ccggcgcctg gaagaccttc
     4201 gatcaggctg acgcgctgga cagaatctat accgtcatgg gcgccgcgat cggcggcatc
     4261 gggggagcgt tccttttcct ggcgctggtc ttgcgcctta agggacaatg acgggcctca
     4321 tcggcccgcc ctgacgatca ggccgctttt ctcgatttcg ccgccggacg tggaattttc
     4381 agccccggcg caaatcggct tccgcaaagt cgagcgctct ggcgatgcgg tcgcaggcca
     4441 tgtcgatggt ggcgcgcgtc cagatcagcg gcggcgacag gatcatcgta tcgccggttg
     4501 cgcgcaacat cagcccttgt gcaatcgcat ggtcgcgcac cgtcaccgcc gcgctgcccg
     4561 ccggcaggaa gcgctccttg gctgccttgt ccttgacgat ttcgatggcg cccatcaagc
     4621 caaaagccct cacctcgcca accagcgcat ggccgccaag gcgctcctgc aaggttttag
     4681 cgaaataagg accggtatcg gtccttaccc gctccaccag cccctctttc tctatgattt
     4741 cgaggttctt caacgccacc gcgcaagcga ccggatgacc ggaataggta tagccgtggt
     4801 tgaactcgcc gcccttctcg accagcgtcg cggcgatgcg gtcgcccacc agcagcgccg
     4861 acagcggctg atagccggag gtcagcgcct tggcggtggt gatggtgtcc ggctcgatgc
     4921 cgaaggactg cgccgcgaac cactcgccgg tgcggccata gccggtgatg acctcgtcga
     4981 gcatcaaaag cacgtcgtat ttgcggcaga tgcgctgcac ttccggccaa tagctcatcg
     5041 gc
//
LOCUS       DASYCS010000089         6789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3642007,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000089 DASYCS010000000
VERSION     DASYCS010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 6789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6789
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3642007"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VGM46_03085"
     CDS             <1..193
                     /locus_tag="VGM46_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4191608.1"
                     /translation="VIGGTSLFGAIGSVHGPLLGAFILATINNGANLLNVNSFWQRVI
                     TGALIIIIVYFDGLRRSRK"
     gene            465..2075
                     /locus_tag="VGM46_03090"
                     /pseudo
     CDS             465..2075
                     /locus_tag="VGM46_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017957455.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            2179..3165
                     /locus_tag="VGM46_03095"
     CDS             2179..3165
                     /locus_tag="VGM46_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008597639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4191609.1"
                     /translation="MTRASTLLRRRLVGSIFVLAIVIVGTFLLLEAAPGDAVDAYAAS
                     TGGDAGLIELLRHRWGLDQSALTRLASYIWTLAHLDLGQSIAFSRPIRDVIFERLPNT
                     LLLMGSATALSFGLGSALGIVAGARPGSFRDRFLSIGSLALYAVPGFWLGLVLIVVFA
                     VDLRWLPIGGVETIASTDTGFARAADIARHLLLPVTALGFIYLALYLRMMRAGMAEVW
                     RMDFILAARARGITRRRIVFAHVARNALLPLITMLGLQSAQMLGGSVVIESVFSVPGL
                     GRLAQEAVTARDTPLLMGIILTSAVLVIIINLAVDIAYAFLDPRVGASEGSP"
     gene            3162..3992
                     /locus_tag="VGM46_03100"
     CDS             3162..3992
                     /locus_tag="VGM46_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006200317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4191610.1"
                     /translation="MSGVSRFLGKPEAIAGSIILCALLAMALSAGLLFPSDPQAIAGP
                     ALLPPFQHMSLPLGTDRLGRNVLAELFHGARASLAVGLAAAAASLAIGAFVGTLAGFA
                     GGLMDEILMRVTDAFQTVPGFLLALAFVSIAGPSLTAVVTAIALSAWTGPARIARAQV
                     LSIRERDYIASARVIGMHPLEIAFREILPNALPPVLALSSIIVAGAILTEAALSFLGL
                     GDPNRVTWGGMIAEGRTVLRSAPFLSIIPGVALVFTVLGVYLAGEGVAEATAIRRGLS
                     "
     gene            3989..4819
                     /locus_tag="VGM46_03105"
     CDS             3989..4819
                     /locus_tag="VGM46_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4191611.1"
                     /translation="MSASGQEVLVSIRDLSVSYSSEADNEAALKGISLDIRDGERLAI
                     IGESGSGKSTFALAIAGLLPGAAKMTGSIEWPGFSTKLGQKPLGGRDIGFVFQDPSAS
                     LDPVMPIGRQIAEVAKTHLFMSWPQAYGHAEKLLKRVRLDPASIIPAYPHQLSGGQKQ
                     RAAIAAAIAAGPNLLIADEATSALDTIVQAEVVSLIEQLVNEDGMALLFVTHDIALAA
                     RLAERIAVFQRGEMVELGETANIIHSPRHACTRTFRDAHIGLDTAPLLGRPAGGPRHG
                     "
     gene            4812..5600
                     /locus_tag="VGM46_03110"
     CDS             4812..5600
                     /locus_tag="VGM46_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY4191612.1"
                     /translation="MDRPVLTVNALTRRFARRGKPITAVDDVSFYIEPAETLALAGPS
                     GSGKSTIARLLLRLIEPDAGTINFDGEDFLALKGAALRRQRAKIQMVFQDPLAAFNPR
                     ATVAGVLGDPLRIHGIASKADRPRHITRLLERVGLSTSLQHRAMHEISGGQRQRVAIA
                     RAIATKPLLVVLDEALSALDVSVRGKILELLLDLQREEKLAYLFISHDLGVVRSIAHR
                     IAILDSGKIAEEGNARTVIDAPQSVVGKALVAAVPTLAADVSHR"
     gene            complement(6184..6260)
                     /locus_tag="VGM46_03115"
     tRNA            complement(6184..6260)
                     /locus_tag="VGM46_03115"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6224..6226),aa:Met,seq:cat)
     gene            complement(6387..6501)
                     /gene="rrf"
                     /locus_tag="VGM46_03120"
     rRNA            complement(6387..6501)
                     /gene="rrf"
                     /locus_tag="VGM46_03120"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(6585..>6789)
                     /locus_tag="VGM46_03125"
     rRNA            complement(6585..>6789)
                     /locus_tag="VGM46_03125"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         1273 a         2250 c         1925 g         1341 t
ORIGIN      
        1 ggtgatcggc ggcacctcgc tgttcggcgc catcggctcg gtacacggcc cgctgcttgg
       61 cgccttcatc ctcgccacca tcaacaatgg cgcgaacctc ttgaacgtca attcgttctg
      121 gcagcgcgtc atcaccggcg ccctcatcat catcatcgtc tatttcgacg gactgcgccg
      181 cagccggaaa tagaccggcg aaagccgctc ccattccgcc cgaaccttcg gatcgaaccg
      241 aaccttccat tccccttgcc cgtgcgaagc tcctgcggca ggcaaaaccg ctttcgcccg
      301 ccgccgcttg acggggcgct cctggaccta tgaattggac caggaaaaga atttctcaaa
      361 gccaccgcct attgagcggg tcgctcctct agcgaaatgt cccgcagcgg tgataatcgc
      421 ggcggacgcc aaaatattcg cgggcgagtt caaatggagc agtcatgtcg cacctgagaa
      481 tttcaagacg caccgtgctt tcaggctccg ccctgttgct cgcttccacg gcattgccgt
      541 ccaccggttg ggcaggcgcg ccgaagaaag gcgggcgtct catcgtcgcc gccgattccg
      601 aaccgcgtaa cctcaacccg gccatcgtcg cttccaacgg cgtctttttt gtttcgagca
      661 aggtggtcga gacgctggca gaagccgact acactggcaa ggatggcctc gcaccccggc
      721 tggcgctcag ctgggaaggc gcggcggacg gcctgtcggc caccttcaaa ttgcgcgaag
      781 gcgtaaaatg gcatgacggc aagccgttca ccgccgccga tgtcgccttt tgcgcccttc
      841 agctatggaa gccgctgcaa aaccttggcc gcaccgtctt caaggacctg gaaacggtcg
      901 agacgccgga cgaccacacc gccatcttca aattctcccg gccaacgccc ttccagctgg
      961 tccggaacgc gctgcccgca ctgtcgagcg tcgtgccgaa acacctctat gaaacgggcg
     1021 atatcgccac caatccggcc aataatgcgc caatcggcac cggccccttc gtattcgccg
     1081 agcaccggcc cggcgaatat taccgcctga cgaagaacgc tgcctagtgg ggcgacggcg
     1141 aaccctatct cgacgaggtc atctatcagg tgctgccgga ccgcgcctcg gcggccaacg
     1201 cgcttgaagc gggagaaatc cagctcgccg ctttctccgc cgtgcctctg gccgatctcg
     1261 accgcatttc gaaagtgccg gggctgaagg tcgtcaccaa gggctacgag gcgctcacct
     1321 atcagctggt ggtcgaaatc aaccatcgcc gcaaggaatt ggcggacctg aaagtccgcc
     1381 aggccatcgc ccatgccatc gacaaggatt tcgtcgtcaa gacggtgttt ctcggctacg
     1441 ccgccacctc caccggcccc gtgccgaaga acgatccgca attctatacg cccgatgtag
     1501 aagcctatcc gttcgatgtc accagggcca acgccctgct cgacgaggcc ggctacgaac
     1561 gtgacggcga cggcaatcgc ttttcgctaa aacttctgcc ggcgccctat ttcaacgaaa
     1621 cccggcaatt cggcgattat ttgcgccagg cgctggccgc cattggcatc gacgcgcaat
     1681 tggtcaacaa tgacgacgcc gcccacatca aggccgtcta taccgaccat gccttcgatc
     1741 tggccgtcgg cccgccggtt ttccgcggcg accccgcaat ctccaccacc atcctcgtcc
     1801 aaagcggcat cccggatggc gtccccttct ccaaccaggg cggctacaaa aatgccggga
     1861 tcgacgcgct gatcgacaag gcgtcgcaaa cgctcgatgc cggcgcgcgc accgaactct
     1921 acagacaatt ccagaagaaa gtggtagccg acctgccgct tatcaacgtg gccgaatgga
     1981 gctttatctc ggtcgtcagc gacaggctga agaacgtcgc cgacaatccg cgctgggcgg
     2041 tgtccaactg ggccgatagc tggctggacg cctgagcttg cgcggacttg cgcgccacag
     2101 gcatgaatgc tcatttccgg cgatgaaccg ttccgatccg gattcgccag acgcagcctt
     2161 tccaggcgcc aaccggccat gacccgcgct tcaacccttc tgcgccgccg cttggtcggg
     2221 tcgatcttcg tgctcgccat cgtcatcgtc ggcacttttc tgctgctgga agcagccccc
     2281 ggcgatgcgg tagacgccta tgccgcctcg accggcggcg atgccggtct gatcgaattg
     2341 ttgcgccacc gctggggtct cgaccaatcg gcgctgacgc gcctcgccag ctatatctgg
     2401 acgcttgccc atctcgatct cggccaatcg attgccttct cgcggccgat ccgcgacgtc
     2461 attttcgaac gcctgcccaa cacgctgctt ttgatgggca gcgccaccgc tctttccttc
     2521 gggcttggct cggcgctcgg catcgtcgcc ggtgccaggc cgggcagttt ccgcgaccgg
     2581 ttcctgtcca tcggatcgct ggcgctttat gcggtgcctg gcttctggct tggcctcgtg
     2641 ttgatcgtgg tcttcgcggt cgatctgcgc tggttgccga tcggcggcgt cgaaaccatc
     2701 gcctcgaccg acaccggctt tgcccgcgcc gccgacattg cccgccacct gctgctgccg
     2761 gtgacagcgc ttggtttcat ctatctggcg ctttacctgc gcatgatgcg cgccggcatg
     2821 gccgaggtct ggcgcatgga tttcatcctt gccgcccgcg ccaggggcat tacccggcgg
     2881 cgcatcgtct ttgcccatgt cgcgcgaaac gcgctgctgc cgctcatcac catgctgggt
     2941 ctgcaatccg cgcagatgct gggcggcagc gtcgtcatcg aaagcgtgtt ttcagtcccc
     3001 ggcctcggcc gcctggcgca ggaagcggtc accgcgcgcg acacgccatt gctgatgggc
     3061 atcatcctca ccagcgcggt gctggtcatc atcatcaatc tggcggtcga tattgcctac
     3121 gcattcctcg atccgcgtgt cggcgccagc gagggttcgc catgagcggc gtttcccgct
     3181 tcctcggcaa gccggaggcc atcgccggct caatcatcct gtgcgcgctc ctcgccatgg
     3241 cgctgtcggc agggcttttg tttcccagcg atccgcaagc aattgccggt ccggccctgc
     3301 tgccgccatt tcagcatatg tcgctgccgc ttggcaccga ccggcttggc cgcaatgtgc
     3361 tggccgagct tttccatggc gcgcgcgcct cgcttgccgt cgggcttgcg gcggcggcgg
     3421 catcgctcgc catcggcgca ttcgtcggca cgctggccgg ctttgcgggt ggactgatgg
     3481 atgaaatact aatgcgggta accgatgctt tccagacggt gcccggcttt cttttggcgc
     3541 tggctttcgt cagcattgcc ggcccctcgc tcacagcggt cgtcaccgcc atcgcgcttt
     3601 cggcctggac cggtccagcc cgcatcgcgc gggcgcaagt actgtccatc cgcgagcgcg
     3661 actatatcgc cagcgcccgc gtcatcggca tgcatcccct cgaaatagct ttccgcgaaa
     3721 ttctgcccaa cgcgctgccg ccggtgctgg cgctgtcctc gatcatcgtc gccggagcga
     3781 tactgaccga ggcggcgcta tcctttctcg gccttggcga tcctaaccgc gtgacatggg
     3841 gcggcatgat cgccgaaggg cgaacagtac tgcgctccgc cccgttccta tcgatcattc
     3901 caggtgtggc cctggttttc accgttctcg gcgtctatct ggcgggcgaa ggcgtcgccg
     3961 aggcgaccgc gatccgccga ggcctgtcat gagcgcctcg ggccaggaag tgcttgtttc
     4021 cattcgcgat ctttccgtga gttattccag cgaagccgac aatgaggccg cgctgaaagg
     4081 catcagcctg gacattcgcg acggcgagcg tctggcgatc atcggcgaaa gcggctctgg
     4141 aaaaagtaca tttgccttgg caattgccgg cctgctgccg ggcgcggcaa aaatgaccgg
     4201 cagcatcgag tggccgggct tttcgacaaa gcttggccaa aagccgctag gcgggcggga
     4261 tatcggcttc gttttccagg acccttcggc cagcctcgat ccggtaatgc cgattggccg
     4321 ccaaatcgcg gaagttgcca aaacccatct tttcatgagt tggccgcagg cctacggcca
     4381 cgcggaaaaa ctgctcaaac gtgtgcgcct cgacccggca tcgatcatcc cggcctaccc
     4441 gcatcagctt tccggcggcc agaaacagcg agcggccatc gccgcggcaa ttgccgccgg
     4501 cccaaatctt ttgattgccg atgaagcaac gagcgcgctc gataccatcg tgcaggccga
     4561 ggtcgtcagc ctgatcgaac agttggtgaa cgaagacggc atggcgttgc tgttcgtcac
     4621 ccacgatatt gcgctcgccg cgcgactggc cgaacgcatc gcggtatttc agcgcggcga
     4681 aatggtggaa ctgggtgaaa cagccaacat catccattca ccacgccatg cctgtacgcg
     4741 gacgttccgc gacgcccata tcggccttga taccgcgccg ctgcttggtc gaccggcagg
     4801 cggaccccgc catggataga ccggtgctga ccgtcaacgc attgaccagg cgcttcgccc
     4861 gccgcggcaa gccgatcact gccgtggacg atgtttcctt ttatatcgaa cctgccgaaa
     4921 cgcttgcgct ggccggccca tccggcagcg gcaaatcgac gatagcaagg ctgctgctgc
     4981 ggctgatcga accggacgcc ggcacgataa actttgacgg cgaagatttt ctggccctga
     5041 aaggcgccgc cctgcgcagg cagcgggcaa aaatacagat ggtattccag gacccgctcg
     5101 ccgccttcaa cccgcgcgcc acggtagcgg gcgtgctggg cgatccgttg cgcatccatg
     5161 gcattgcgtc gaaggcggat cggccgcgcc atattacgag gctgctggaa cgcgtcggcc
     5221 tgagcaccag tttgcagcat cgcgccatgc atgaaatatc cggcgggcaa aggcagcggg
     5281 tggccattgc acgcgccatc gcgacgaaac cactgctggt tgtgctcgac gaagcattgt
     5341 cggcgctgga tgtgtccgtt cgcgggaaaa ttctcgagct tctgctcgac cttcaacgtg
     5401 aagaaaagct ggcctatctg ttcatttcgc acgatctggg cgtcgtccgc tccatcgccc
     5461 accgcatcgc catcctcgat agcgggaaaa ttgcagagga gggcaacgcg cgaaccgtta
     5521 tcgacgcccc gcaatcggtg gttggcaagg cgcttgtcgc ggcggtccca accctcgcgg
     5581 ctgatgtttc gcaccgttaa caggctcacc gcagccttgc cctcgatgct tcccatattt
     5641 cccgcttcag ttcgtccata acttccgttg catacccaat gcgccatcat gccccggagc
     5701 aacagcgcac atcgcccact gagaatacaa gaccaatccg cgcctcgaaa agcatgcctg
     5761 acgagaagat ggaaaaatcc gtgaaacacc gattgcatgt aacttccgac gggccacggt
     5821 ttccgccgcg ccaaacttcc gccaaatcga caacaagcga cgggcgaaag cccaccagcc
     5881 ggattacccg atgcatagac catgcagttc gaataaatgg cggcagccag caagacgaca
     5941 gacccaacat cgcgccatct ttccgagaca cgccatcttg ccatcaagcg gcaacaaaac
     6001 agcgccgaca accagtgcca tttgagttgg attgcgggga caggatttga acctgcgatc
     6061 ttcggcctat gaacctgatg aggtgaggcc gaccttgcgt cgacccagtt ggtcgatgcc
     6121 ggccgaacgg gctgttccca agcacaaaaa aagcccgccg acttacgtca acgggcttga
     6181 atttggttgc ggggacagga tttgaacctg tgaccttcag gttatgagcc tgacgagcta
     6241 ccgggctgct ccaccccgcg tcaaccttac agataggcat tcgcccatcg aattccaacc
     6301 cgccaatgaa aaggccgctt gcgcggccca atttcccgtt tggcgggcca tttcatgaag
     6361 agaagattta aacgtgcgct ttgtagacct ggcagcgact tactctcccg cgtcttgaga
     6421 cgaagtacca tcagcgctgg aacgtttcac ggccgagttc ggaatgggat cgggtgcagc
     6481 cgttccgcca taaccaccag gtcggcaaaa cgcacgttat atctcgagaa gctgttgtct
     6541 ttgcgtgcca tccatcgttt tcacgatggg cattcgaaat gagaacgatc aagccgatcg
     6601 aactattagt actggtaagc ttcaagcgtt gccgctcttc cacacccagc ctatcaacgt
     6661 ggtcgtcttc cacggttctc agggaatact cgttttaagg tgggtttccc gcttagatgc
     6721 cttcagcggt tatcccgtcc ggatatagct accctgctat gcggctggcg ccacaacagg
     6781 tccaccaga
//
LOCUS       DASYCS010000090        10561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_743321,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000090 DASYCS010000000
VERSION     DASYCS010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 10561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10561
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743321"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="VGM46_03130"
     CDS             complement(<1..559)
                     /locus_tag="VGM46_03130"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528054.1"
                     /note="NADP-dependent; catalyzes the oxidative
                     decarboxylation of malate to form pyruvate; decarboxylates
                     oxaloacetate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEY4191613.1"
                     /translation="MARKDRHAEQGPSVSAEEALEFHAMGRPGKLEIVATKPMATQRD
                     LSLAYSPGVAVPVQAIADDPSRAFDYTTRGNMVAVISNGTAILGMGNLGALASKPVME
                     GKAVLFKRFADVDSIDLEVDTTDVDAFIGAVRYLGPSFGGINLEDIKAPDCFVIEERL
                     RELMDIPVFHDDQHGTAIISAAGVIN"
     gene            824..3523
                     /gene="mutS"
                     /locus_tag="VGM46_03135"
     CDS             824..3523
                     /gene="mutS"
                     /locus_tag="VGM46_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006325159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEY4191614.1"
                     /translation="MPAAAGGVTPMMEQFIEIKAANPGSLLFYRMGDFYELFFDDAEK
                     ASKALGIVLTKRGKHQGADIPMCGVPVHAADDYLQKLIGQGFRVAVCEQIEDPAEARK
                     RGGKSVVRRDVTRLVTPGTITEDKLLAPSESSFLMALGRVKGAAESSSFALAWIDIST
                     GAFRVTDTTAERLLADIFRIDPRELIVAEPVFHDPELKAVFDVLGRVASPQPPSLFDS
                     TFAPARIARFFEVATPDSFGAFSRAELSAVSGIIAYVEKTQKAERPPLSRPEREEQGS
                     SLFIDPATRANLELLRTLSGSREGSLFKAIDRTVTGGGARLLADRLMAPLTDPDAISA
                     RLDSVSFFRNEVRLCQSVRLALKGVADMPRALTRLALNRGGPRDLGALRAGFQAAADI
                     AEIFAGAALPHELQQALAAIAALPQALGRHLTQALGEELPLLKRDGGFLRAGYHADLD
                     EMRALATQSRKVIAGLERGLADETGIKSLKIRHNNVLGYYIEVTANHAGAMTGTDAAR
                     GKFIHRQTMANAMRFTTTELAELETKIANAADRALTIELAAFDALVREAVGEAQNIRA
                     GAEALSVLDVSAALALLSESENWCRPVVDSSLAFEIIGGRHPVVEQALRRSAEGSFVA
                     NDCDLSPEGDARGGKEAGAIWLLTGPNMGGKSTFLRQNALIAILAQTGSYVPAASAHI
                     GVVDRLFSRVGASDDLARGRSTFMVEMVETAAILNQAGARALVILDEIGRGTATFDGL
                     SIAWAAVEYLHEKNRCRAVFATHFHEMTALAGKLSRLHNVTMRVKEWEGDVVFLHEVG
                     KGAADRSYGVQVARLAGLPDAVVARAREVLHQLEEGEVSGKTDRLVDDLPLFSVAAKR
                     EAPKPAGPARADALGEALAKVNPDEMTPREALEALYRLKGLAV"
     gene            3593..4057
                     /locus_tag="VGM46_03140"
     CDS             3593..4057
                     /locus_tag="VGM46_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003605301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1348 family protein"
                     /protein_id="HEY4191615.1"
                     /translation="MSRPPLPPFTAETAALKVRLAEDAWNGRDPDKVSTGYSEDSVWR
                     NRAEFVTGRAEIVGLLRRKWVRELDYRLIKEVWAWHENRIAVRFAYEWHDDAGQWYRS
                     YGNENWRFNADGLMAHRHASINDLPIRADERKFHWPLGRRPDDHPGLSDLGL"
     gene            4066..4623
                     /locus_tag="VGM46_03145"
     CDS             4066..4623
                     /locus_tag="VGM46_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012254412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY4191616.1"
                     /translation="MGRTAPDKDGLLATLAEVFREHGYEGTSLARIGHATGLGKGSLY
                     NFFPGGKEEMAGAVLANIDAWFDENVFAPLCDECEPAAAIGRMLDATDLYFRSGRRAC
                     LLGAFALDDSRDRFGNEIKSYFDRWIASLAGALMRAGHRQPEARQLAEDTVAGVQGAL
                     TLARAFDEPEVFSRSLKRMRERLVA"
     gene            complement(4701..5540)
                     /locus_tag="VGM46_03150"
     CDS             complement(4701..5540)
                     /locus_tag="VGM46_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013891429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY4191617.1"
                     /translation="MPSTMRMTTLPSGEAVPVFGLGTWKMGEDPRQADAEADALRVGL
                     DLGVTLIDTAEMYGNAEAVVAEAIAGRRDGLFVVSKVLPSNASRSGTVAACERSLKRL
                     RTDRIDLYLLHWRGGVPLGETVEAFETLKSAGKIRHWGVSNFDADDMEELAALPAGAN
                     CQTNQVLYNLSRRRPEFDLAPWCRQRGMPIMAYSPIEQGRLALSDKLAAIAARHNATP
                     AQVALAWTIRGQGVIAIPKASRPAHVRQNMAALDIKLSADDLTELDRAFPPPARKRPL
                     EMI"
     gene            complement(5644..6021)
                     /locus_tag="VGM46_03155"
     CDS             complement(5644..6021)
                     /locus_tag="VGM46_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015314168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEY4191618.1"
                     /translation="MSMVRKIVLAPAVAALLMAMPAQSAQMKHDMGAMGEAAGDPAAA
                     GYQAAMDKMHREMMAMKYTGDADVDFVRGMIPHHQAAIDMANVLLAHGKDPEMRKLAE
                     NIVAAQQKEITEMENWLAAHPVK"
     gene            complement(6058..8595)
                     /locus_tag="VGM46_03160"
     CDS             complement(6058..8595)
                     /locus_tag="VGM46_03160"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015341555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEY4191619.1"
                     /translation="MANHHRDATHHGHEADGSCCSGHAAARPDLPGLAQAVIRDPVCG
                     MTVDAQAGKPGIEHGGRMFHFCSDGCRAKFAAHPDQYLHARDPVCGMEVDRASAAHFL
                     RHAGEKFYFCSAGCKARFEAEPEKYLGERPAAPAMPKGTQYTCPMHPEIVRDRPGDCP
                     ICGMALEPMGVPAGEAGPNLELVDFSRRLWISAALSMPLLVLSMGPMLGLTFFRDRLG
                     EPAATWVEFALASPVVLWAALPFFRRGWQSILNRSANMWTLISLGVGAAYLYSVVATL
                     FPGIFPHALRGHDGAVPVYFEACAVIVALVFLGQVLELRAREKTGSAIRALLDLAPKT
                     ARRVAADGSESDVPLDSVRKGDLLRIRPGEAVPVDGAVEDGRSSIDESMITGEPLPVE
                     KVQGDTLTGGTLNRNGTLLMRAQRIGSETTLSRIVELVAKAQRSRAPVQGLADRVSFY
                     FVPAVVTVAIVAFVAWAVFGPEPGLVHAIVAAVSVLIIACPCALGLATPMSIMTATGR
                     GAQAGVLVKEAAALERLASVDTLIVDKTGTLTEGRPALTDVVAMDGFSETGLLALAAG
                     LEKGSEHPLAEAIVEGAKARGAQAAEVSGFEAVTGKGVSGTVSDGVAALGNAAMMADL
                     GIDISHLGARALALQTEGKTAMFVAMGAKLAGIVAVADPIKASAAEAIQLLREQGLTI
                     IMATGDNERTAQAIAQRLGIERVHAGLSPEAKAALVEELQARGCAVAMAGDGVNDAPA
                     LSTADVGIAMGAGADVAVESAGITLVKGDLNGIVRARKLARATIGNIRQNLFFAFLYN
                     LLGVPVAAGVLYPLTGTLLSPMLAAAAMSLSSVSVIANALRLRTLKL"
     gene            8807..9589
                     /locus_tag="VGM46_03165"
     CDS             8807..9589
                     /locus_tag="VGM46_03165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY4191620.1"
                     /translation="MDARTRRILVRTLLAIGPAVVATFAISFSLRWALGMPIDWLSWI
                     ECAAIPIVVGMPIGVYIFSQAERLQSAHERLERVHQDMVKAHERLAFMASHDKMTGLL
                     NRDGFLGEVEPYRQRNEGHVLLIIDADHFKRINDRYGHPKGDEALMKIAKALRYAVRP
                     KDVVGRIGGEEFAILLVSVGMREATRMAELVRRQVQCIPWQVEGPETPQLTVSIGGAA
                     LEWRHATLAETLREADRNLYEAKRLGRNRVVFDHAGTATEAA"
     gene            9681..>10561
                     /locus_tag="VGM46_03170"
     CDS             9681..>10561
                     /locus_tag="VGM46_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEY4191621.1"
                     /translation="MAKISLKLDELIDSEALRRDLAAVAGTDAAQTRAAALKLLKERR
                     AIAAGKAEAMLMTDGSGTACAVRLSHVMDEIIRALHDFAVAQAKTGPERMALVAVGGY
                     GRGTLAPGSDIDLLFLLPAKQTASGAHIVETVLYMLWDMGLKVGHATRTVDECLRLSR
                     DDITIRTALLEARFIWGDEPLCQTLLQRFDRELVRTTGPEYVQAKLAERDARHAKAGE
                     SRYLVEPNVKDGKGGLRDLHTLFWIGKYFYRVRTGEELVEKGVFTQGEHNQFRKAEDF
                     LWAVRCHMHFLTGKPEER"
BASE COUNT         1814 a         3479 c         3450 g         1818 t
ORIGIN      
        1 cgttgatcac gccggcggcg gagatgatcg cggtgccgtg ctggtcgtca tggaacaccg
       61 ggatgtccat cagctcgcgc aggcgctcct cgatcacgaa gcagtccggc gccttgatat
      121 cctccaggtt gatgccgccg aagctcggcc ccagatagcg caccgcgccg atgaaggcgt
      181 cgacgtcggt ggtgtcgact tccaggtcga tggaatcgac gtcggcgaag cgcttgaaca
      241 gcaccgcctt gccctccatc accggcttgg aggcgagcgc gccgagattg cccatgccca
      301 ggatggcggt gccgttggag atgacggcga ccatgttgcc gcgcgtcgtg tagtcgaagg
      361 cgcgcgaagg atcgtcggcg atcgcctgca ccggcacggc gacgcccggc gaataggcca
      421 gcgacaggtc gcgctgggtg gccatcggct tggtggcgac gatttccagc ttgccgggcc
      481 ggcccatggc gtggaattcc agcgcttcct cggcgctgac ggatggaccc tgctcggcgt
      541 gcctgtcttt ccttgccatc tctgctcttt cctcccgagc ggtattgcgc ttgtagtttc
      601 gttgtggagt gctcccgatt aaggctacac tttacaaacg taaatggcca agcaggggcc
      661 ggcgaaattt ggaaacagtt tcttgaacga cgaaacctcg atccgggtcg aaaccgcggc
      721 cgcccatggc gccggccccg ttgccacagg cggtgctgcc ctaacgccag ccgccacagg
      781 cggcgttacg ccaatgccag ccgccgcagg cggtgtaacc ccaatgccag ccgccgcagg
      841 cggcgtaact ccaatgatgg agcagttcat cgagatcaag gcggcgaatc ccggttcgct
      901 gctgttctac cggatgggcg atttctacga gctgttcttc gacgatgccg aaaaggccag
      961 caaggcgctg ggcatcgtcc tgaccaagcg cggcaagcac cagggcgccg atattccgat
     1021 gtgcggcgtg ccggtccatg ccgccgacga ctatttgcaa aagctgatcg ggcaggggtt
     1081 tcgcgtcgcc gtctgcgagc agatcgaaga cccggccgag gcgcgcaaac gcggcggcaa
     1141 atcggtggtg cgccgcgacg tcacgcggct ggtgacaccg ggcaccatca ccgaggacaa
     1201 gctgctggcg ccttcggaat cgagcttcct gatggcgttg gggcgggtga agggcgctgc
     1261 cgaatcttcc tctttcgcgc tggcgtggat cgacatttcc accggcgctt tccgcgtcac
     1321 ggatacgacc gccgagcgcc tgctggccga tattttccgc atcgatccgc gtgaactgat
     1381 cgtcgccgag ccggtgttcc acgaccccga actgaaagcc gtcttcgacg tgctgggccg
     1441 cgtcgccagc ccgcagccgc ccagcctgtt cgattcgact ttcgcgccgg cgcgcatcgc
     1501 ccgcttcttc gaggtggcga cgccggacag tttcggtgcc ttcagccgcg cggagctgtc
     1561 ggcggtgtcg ggcatcatcg cctatgtcga gaagacccag aaggccgagc gcccgccctt
     1621 gtcgcggccc gagcgcgagg agcagggttc gagcctgttc atcgacccgg cgacccgcgc
     1681 caatctggaa ttgttgcgca cgctttccgg cagccgcgag ggttcgctgt tcaaggccat
     1741 cgaccgcacg gtgaccggcg gtggcgcgcg gctgctggcc gaccggctga tggccccgct
     1801 gaccgacccg gatgccattt cggcgcggct ggattcggtc tcgttcttcc gcaacgaggt
     1861 gcggctgtgc cagtcggtgc ggctggcgct gaagggcgtc gccgacatgc cgcgcgccct
     1921 gacgcggctg gcgctcaacc gtggtggccc gcgcgacctt ggcgcgttgc gcgccggctt
     1981 ccaggcggcg gccgatattg ccgaaatttt tgccggcgcg gcactccccc acgaactgca
     2041 acaggccttg gccgcgattg ccgccctgcc gcaagccctt ggccggcatc tgacgcaggc
     2101 gctgggtgag gaactgcctt tgttgaagcg cgatggcggc ttcctgcgcg ccggctacca
     2161 tgccgatctg gacgagatgc gcgcgctggc cacccaatcg cgcaaggtga tcgccgggct
     2221 ggaacgcggc ctggccgacg agaccggcat caagtcgctg aagatccgcc acaataatgt
     2281 actgggctac tatatcgagg tgaccgccaa ccatgccggg gccatgaccg gcaccgacgc
     2341 ggcgcgggga aaattcatcc accgccagac catggccaac gccatgcgct tcaccaccac
     2401 cgaactggcg gaactggaaa cgaaaatcgc caacgccgcc gaccgcgcgc tgaccatcga
     2461 gcttgccgcc ttcgacgcgc tggtcaggga agcggtgggc gaggcgcaga atatccgcgc
     2521 cggcgccgag gcgctttcgg tgctcgacgt gtcggctgcg ctggcgcttc tttccgagag
     2581 cgagaactgg tgccggccgg tcgtcgattc cagccttgct ttcgagatca tcggcggccg
     2641 ccatccggtg gtcgagcagg cgctgcgccg ctccgccgag ggatcgttcg tcgccaacga
     2701 ttgcgacctg tcgccggaag gcgatgcccg aggaggcaag gaggccggcg cgatctggct
     2761 gttgaccggc cccaatatgg gcggcaagtc gaccttcctg cgccagaacg cgttgatcgc
     2821 catccttgcc cagaccggct cctacgtgcc ggcggcaagc gcccatatcg gcgtggtcga
     2881 ccggctgttt tcgcgcgtcg gcgcatccga cgacctggcg cgcgggcgct cgaccttcat
     2941 ggtcgagatg gtcgagacgg cggcgatcct caaccaggcc ggcgcgcgcg ctttggtcat
     3001 actggacgag atcgggcgcg gcaccgccac cttcgacggc ctctccatcg cctgggcggc
     3061 ggtggaatat ctgcacgaga agaaccgctg ccgggcggtc ttcgccacgc atttccacga
     3121 aatgacggcg ctggccggaa agctttcgcg cctgcacaac gtcaccatgc gggtgaagga
     3181 atgggagggc gacgtggtct tcctgcacga ggtcggcaag ggcgcagccg accgctccta
     3241 cggcgtccag gtggcgcgcc tcgccggcct gcccgacgcg gtcgtggcgc gcgccaggga
     3301 agtcctgcac cagcttgagg agggcgaggt ttccggcaag accgaccggc tggtcgacga
     3361 tctgccgctg ttttcggtgg cggcgaagcg cgaagcgccg aagcccgccg gccccgccag
     3421 ggccgacgcg ctgggcgagg cgctggccaa ggtcaatccc gacgagatga cgccgcgcga
     3481 ggcgctggag gcgctctatc gcctgaaggg cctggcggtt tgaatggcga tgttgacagg
     3541 tttgtaccga ttggtacaat aaacgccatc accgttttga tggaagaaca ccttgagccg
     3601 tccgccgctg ccgcccttca ccgccgaaac cgccgccctg aaggtgcgcc tggccgagga
     3661 cgcctggaac gggcgcgacc cggacaaggt atcgaccggc tatagcgagg acagcgtctg
     3721 gcgcaaccgc gccgaattcg tcaccggccg cgccgagatc gtcggcctcc tgcgccgcaa
     3781 atgggtgcgg gaactggact accggctcat caaggaagtc tgggcttggc acgaaaaccg
     3841 catcgccgtg cgcttcgcct atgaatggca tgacgatgcc ggccagtggt atcgttccta
     3901 cggcaacgag aactggcggt tcaatgccga cggcttgatg gcccatcgcc atgccagcat
     3961 caacgatctg ccgatcaggg cggatgaacg caagttccat tggccgctcg gccgccgtcc
     4021 cgacgaccat ccgggccttt ccgatctggg cctctaggcg cttatatggg caggaccgct
     4081 cccgacaagg acggcctgct ggccacgctg gcggaggttt tccgcgagca tggctatgag
     4141 ggaaccagcc tggcgcgtat tgggcacgcg acagggctgg gcaagggcag cctctacaat
     4201 ttctttcctg gcggaaaaga agaaatggca ggggctgttc ttgcaaacat cgatgcctgg
     4261 ttcgacgaga atgtctttgc gccgctatgc gacgaatgcg aaccggccgc cgccatcggc
     4321 aggatgctgg acgccaccga cctctatttc cgctccgggc gccgcgcctg cctgctgggg
     4381 gccttcgcgc tggacgattc gcgggatcgg ttcggcaacg aaataaagtc ttatttcgac
     4441 cgctggatcg ccagcctggc cggtgcgctg atgcgtgccg gccaccggca accggaagcc
     4501 cgtcaattgg ccgaggacac ggtcgccggc gtccagggcg cgttgacgct ggcgcgcgcc
     4561 tttgacgaac cggaagtgtt ttcccgctcc ctcaaacgta tgcgcgaaag gctggtcgcc
     4621 tgagatacca gagcggttct ggtttttacc gaatcggtaa aaccgctctg tctctttgtt
     4681 ttggccgcat ttccggatgc tcagatcatc tccagcgggc gcttgcgcgc cggcggcggg
     4741 aaggcgcggt caagctcagt gagatcgtcg gctgaaagct ttatgtcgag cgccgccatg
     4801 ttctggcgaa catgcgccgg ccggctggct ttggggatgg cgatgacgcc ttgcccgcgt
     4861 atcgtccagg ccagcgccac ctgcgccggc gtggcattgt ggcgcgccgc aatcgctgca
     4921 agtttgtcgc tgagcgccag ccgcccctgc tcgatcggcg aataggccat tatcggcatg
     4981 ccgcgctgcc ggcaccatgg cgcgaggtcg aattccggcc tgcgccgtga aaggttgtac
     5041 agcacctggt tggtctggca attggccccg gccggcaggg cggcaagctc ttccatgtcg
     5101 tcggcgtcga aattcgacac gccccaatgg cggattttcc cggccgattt cagcgtctcg
     5161 aacgcttcca ccgtttcgcc aagcggcacg ccgccgcgcc aatgcagcag atagaggtcg
     5221 atgcggtctg tgcgcaggcg cttcaggctg cgctcgcagg cggcgaccgt gcccgaacgc
     5281 gacgcgttgg acggcagcac cttggaaacg acgaacaggc catcgcggcg gccggcaatg
     5341 gcttcggcga ccaccgcttc ggcattgccg tacatttccg cggtatcgat cagcgtcacg
     5401 ccgagatcaa ggccaacccg cagcgcatcc gcctcggcgt cggcctggcg cgggtcctcc
     5461 cccatcttcc aggttccaag cccgaaaacg ggaacggctt cgcccgaagg cagggtcgtc
     5521 attcgcatgg tggaaggcat tggcgggctc cgcatcgaag aggtttcaaa ccagtctatt
     5581 ttgctggcca ttctgcttcg ccagccggcc tgtgtcactg gccagcctgc ttaactagaa
     5641 agtctacttc acgggatggg cggcgagcca gttttccatt tcggtgattt ccttttgctg
     5701 ggcggcgacg atgttttcgg ccagcttgcg catttccggg tccttgccat gggcaagcag
     5761 cacgttcgcc atgtcgatgg ccgcctgatg atgggggatc atgccgcgca cgaaatcgac
     5821 atcggcgtcg ccggtatatt tcatcgccat catctcgcgg tgcatcttgt ccatcgccgc
     5881 ttgatagccg gcggcggcgg ggtcgcctgc cgcctcgccc atcgcgccca tgtcgtgctt
     5941 catctgcgcc gattgcgccg gcattgccat caacagcgcc gccaccgccg gcgcaagcac
     6001 gatctttcga accatggaca tgagatttct cctttggaat ctatctggag gatgggttca
     6061 aagtttcaat gtcctgagcc tcagcgcgtt ggcgatgacc gacaccgacg acaggctcat
     6121 cgcggcggcg gccagcatcg gcgacaacag cgtgccggtc agcggataga gcacgccggc
     6181 ggctaccggc acgcccagca ggttgtagag aaaggcgaag aaaaggttct ggcggatatt
     6241 tccgatcgtc gcccgcgcca gctttcgcgc ccgcacaatg ccgttgaggt cgcccttgac
     6301 cagcgtgatg ccggcacttt ccaccgccac atccgcgccc gcacccatgg cgatgccgac
     6361 atcggcggtg ctgagcgcgg gcgcgtcgtt gacgccgtcg ccggccatcg ccacggcgca
     6421 gccgcgcgct tgcagttctt cgacgagcgc cgccttcgcc tcgggtgaaa ggcccgcatg
     6481 cacccgctct atgcccagtc tttgcgcgat ggcctgcgcg gtgcgctcat tgtcgccggt
     6541 cgccatgata atggtcaggc cctgttcccg cagcagctgg atagcctcgg cggcgctcgc
     6601 cttgatcggg tcggcgacgg cgacgatgcc ggcgagcttt gcgcccatag ccacgaacat
     6661 tgccgtcttg ccctcggttt gcagcgcaag ggcacgcgcg ccgaggtgcg aaatgtcgat
     6721 gccgagatcg gccatcatgg cggcgttgcc cagcgccgcg acgccatccg ataccgtccc
     6781 cgaaacgccc ttgccggtaa ccgcctcgaa accgctcact tcagccgcct gtgcgccgcg
     6841 cgccttggcg ccctcgacga tggcttcggc cagcggatgc tcggagcctt tctccaggcc
     6901 ggcggccaat gccagcaatc cagtttcgga aaagccgtcc atggcgacca cgtcggtcag
     6961 cgccggccgg ccttcggtca gcgtgccggt cttgtcgacg atcagcgtat cgacggacgc
     7021 aaggcgttcc agcgccgccg cttccttgac caggacgccg gcctgcgcgc cgcgcccggt
     7081 cgcggtcatg atcgacatcg gcgtcgccag cccgagcgcg cagggacagg cgatgatcag
     7141 caccgacacc gccgcgacga tggcgtggac cagccccggt tccggcccga aaaccgccca
     7201 ggcgacgaag gcgacgatgg cgacggtgac gacggcggga acgaagtaga acgaaacgcg
     7261 gtcggccagc ccctgcaccg gcgcgcggct gcgctgcgcc ttggccacca gttcgacaat
     7321 gcgcgaaagc gtcgtttcgg agccgattcg ttgcgcgcgc atcagcaatg tgccgttgcg
     7381 gttcagcgtt ccgccggtga gcgtgtcgcc ctgcaccttc tcgaccggca gcggctcgcc
     7441 cgtaatcatc gattcgtcta tcgaggagcg gccatcttcg acggcgccgt ccaccggcac
     7501 ggcctcgccg gggcggatgc gcagcaggtc gcccttgcgc acgctgtcga gcggcacgtc
     7561 gctttcgctg ccgtccgccg ccacccgcct tgccgtcttc ggcgcgagat cgagcagggc
     7621 gcgaatggcc gagccggtct tttcgcgcgc gcgcagttcc agcacctggc cgaggaagac
     7681 cagcgccacg atgacggcgc aagcctcgaa atagaccggc accgcgccat catggccgcg
     7741 aagcgcatgc gggaagatgc cgggaaacag cgtggcgacg acgctgtaga gataggccgc
     7801 gccgacgccg agcgaaatca gcgtccacat attggcgctg cggttgagga tcgactgcca
     7861 gccgcgccgg aagaaaggca gcgccgccca cagcaccacc gggctggcca gcgcgaattc
     7921 cacccatgtc gcggccggct cgcccagtcg gtcgcggaaa aacgtcaggc cgagcatcgg
     7981 ccccatggag agcaccagca gcggcattga cagcgccgcg ctgatccaca ggcggcgcga
     8041 aaaatcgaca agttccaggt tcggtccggc ctcgccggcc ggcacgccca tcggctccaa
     8101 cgccatgccg cagatcgggc aatcgcccgg cctgtcgcgc acgatctcgg ggtgcatcgg
     8161 gcaggtatat tgcgtgccct tcggcatcgc cggggccgct ggccgctcac ccaggtattt
     8221 ttccggctcg gcttcgaacc ttgccttgca gcccgccgag cagaaataga atttttcgcc
     8281 cgcatggcgc aagaaatgcg cggcgctggc gcggtccacc tccatgccgc acaccgggtc
     8341 cctggcgtga agatactggt cgggatgcgc ggcgaatttc gcccggcagc catcgctgca
     8401 aaaatgaaac atccgcccgc catgctcgat gccgggtttg cccgcttggg cgtcgaccgt
     8461 catgccgcag acgggatcgc ggatgacggc ttgcgccaat cctggcaggt ccgggcgcgc
     8521 ggcggcatgg ccggaacagc aggagccatc ggcctcgtgg ccatgatggg tggcgtcgcg
     8581 atgatggttg gccatggcgg aaacctcgaa aaataacgcc caaatcagcg ttcggattgg
     8641 atcgtcgccc atagataggg cttccagtag ctggaaggtc aaggcggcaa aaccgacaat
     8701 gccgcgcttc atgaaaatat cggatcgggg cggaaatccg gcaatcgcta aaatgctaaa
     8761 ggatcggcta aaggctattt tacgccgtcg cgctaatggc tctggcatgg acgcacgtac
     8821 aagacgcata ttggtaagaa ccctgctcgc catcggcccc gcggtggtgg cgacctttgc
     8881 gatttccttt tccctgcgct gggcgctggg aatgcccatc gactggctga gctggatcga
     8941 atgcgctgca attcccattg tcgtgggcat gccgatcggg gtgtacattt tcagccaggc
     9001 cgaaaggctc caaagcgcgc atgagcggct ggagcgcgtg catcaggaca tggtcaaggc
     9061 gcatgagcgg ctggccttca tggccagtca cgacaagatg accggcctgc tcaaccgcga
     9121 tggcttcctg ggcgaggtcg agccataccg gcagcgcaat gaaggccatg tcctgctgat
     9181 catcgacgcg gaccacttca agcgcatcaa cgaccgttac ggccatccca agggcgacga
     9241 agcgctgatg aagatcgcca aggcgctgcg ctatgcggtg cggccgaaag atgtcgtcgg
     9301 ccggatcggc ggcgaggaat tcgccatttt gctggtttcc gtcggcatgc gcgaggcgac
     9361 ccggatggcc gagcttgtcc gccggcaggt tcagtgcatt ccctggcagg tggaggggcc
     9421 ggaaacgccg cagctgaccg tcagcatcgg cggggcggcg ctggagtggc gccatgccac
     9481 gcttgccgaa acgctgcgcg aagccgaccg caacctttac gaggccaagc gcctcgggcg
     9541 caaccgcgtg gttttcgacc atgccggcac ggcgacggaa gcggcctagc cgaacggcgg
     9601 agcgccggca gccagcccat acccagcgcg ataaaaacac gctatagacg cggccgaatg
     9661 gcgaacccat ctgcggctct atggcaaaaa tatccctgaa gctcgacgaa ctgattgaca
     9721 gcgaagcctt gcgccgcgat ctcgccgccg tggccggcac ggatgctgcg caaacgcgcg
     9781 ccgccgcgct caagctcctc aaggagcgcc gcgccattgc cgccggcaag gccgaggcga
     9841 tgctgatgac ggacggcagc ggcactgcct gcgcggtgcg cctttcccat gtgatggacg
     9901 agatcatccg cgcgctccat gattttgcgg tcgcgcaggc gaaaaccggc cccgagcgca
     9961 tggctctggt ggccgtcggc ggctatgggc gcggcacgct ggcccccggt tccgacatcg
    10021 acctcctgtt cctgctgccc gccaagcaga cggcctcggg cgcgcacatt gtcgaaaccg
    10081 tgctttacat gctgtgggac atgggcctga aggtcggcca cgccacccgc accgtcgatg
    10141 agtgcctgcg cctgtcgcgc gacgacatca ccatccgcac ggcgctgctg gaggcgcgtt
    10201 tcatctgggg cgatgagccg ctttgccaaa ccctgttgca gcgcttcgac cgcgaactgg
    10261 tgcgcacgac cggtcccgaa tatgtgcagg ccaagcttgc cgagcgcgac gcccgacatg
    10321 ccaaggccgg cgaaagccgc tatctggtcg agccgaacgt caaggacggc aagggcggcc
    10381 tgcgcgacct gcatacgctg ttctggatcg gcaaatattt ctaccgcgtc cgcaccggcg
    10441 aggaactggt cgaaaagggc gtcttcacgc aaggcgagca caaccagttc cgcaaggccg
    10501 aggatttcct gtgggcggtg cgctgccaca tgcatttcct taccggcaag ccggaagagc
    10561 g
//
LOCUS       DASYCS010000091         7249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4223544,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000091 DASYCS010000000
VERSION     DASYCS010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 7249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7249
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4223544"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1133)
                     /gene="deoA"
                     /locus_tag="VGM46_03175"
     CDS             complement(<1..1133)
                     /gene="deoA"
                     /locus_tag="VGM46_03175"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HEY4191622.1"
                     /translation="MLAQEIIRRKRDGQRLSSEEVSAFIAGLTSGSVSEGQAAAFAMA
                     VFFNGMSRDEAVALTLAMRDSGSVLNWSHLPGPVTDKHSTGGVGDNVSLVLAPIVASC
                     GAYVPMISGRGLGHTGGTLDKMDAIPGYVSQPDLTLFRKVVLETGFAIIGQTVDLAPA
                     DRRLYAVRDVTATVESVPLITASILSKKLAAGLGSLVLDVKVGNGAFMEKSRDATALA
                     ASLVEIANGAGLKASALVTGMDEPLACAAGNAVEVRNAVDFLTGRTRDPRLEAVTLAL
                     AAEMLLAAELVSTNQDGLRRAREALEAGRAAALFGRMVAALGGPSDFIERADKYLPRA
                     AVEFAVRATESGFVTGIETRNIGLAVVELGGGRRRPDERIDHSV"
     gene            complement(1161..1967)
                     /locus_tag="VGM46_03180"
     CDS             complement(1161..1967)
                     /locus_tag="VGM46_03180"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEY4191623.1"
                     /translation="MKQAIDMLVERLDGLAPTTALVLGSGLGGLAGQVEDAVRIAYAD
                     MPGFPRASVSGHAGEMVAGRFAGKPILMLAGRTHYYEQGDAAAMRPLLEALAGIGIAK
                     LIVTNSAGSLDPQMLPGSVMLVEDHINFSGLNPLIGEPGERRFVGLTEAYDAQMRTAF
                     EQAAQATGTMLHKGVYMWFSGPSFETPAEIRMARLMGADAVGMSTVPEVILARFLGLR
                     VAACSIITNLAAGMTGAELSHQETKDMAPVGGARLAVILRHVFREGLLDG"
     gene            complement(1964..2380)
                     /gene="cdd"
                     /locus_tag="VGM46_03185"
     CDS             complement(1964..2380)
                     /gene="cdd"
                     /locus_tag="VGM46_03185"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEY4191624.1"
                     /translation="MTADLMTAGPHDLYLAARAAMAKAYAPYSKFPVGAALRTEDGQV
                     FAGANIEVASYPEGWCAETTALGHYVMGGGGKITEIAVVAERMARCTPCGGCRQRLAE
                     FCGPATRLYLCDETGVVETVTMGDILPYGFKGDILK"
     gene            complement(2385..3356)
                     /locus_tag="VGM46_03190"
     CDS             complement(2385..3356)
                     /locus_tag="VGM46_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4191625.1"
                     /translation="MDMFNAIVQTLDSTIRLSVPLLFASLAGLYSERAGIFDIGLEGK
                     MLAGAFAGAAAAAVFHSALIGLGMAVLISVGFALVHGFASITHRGNQIVSGVAINFIA
                     AGSTIILGQAWFQQGGRTPALGAGERFSPITLPGADAVKDVPVIGQLYSELLSGHSIL
                     VYLAFLMVPVSWWVLFRTRFGLRLRAVGENPAAVDTAGISVAWLRYRALICTGILTGL
                     AGAYLSMAQNGGFVKDMTAGKGYIALAALIFAKWKPVNVMFACLLFGFLDALSIRLQG
                     TPLPLIGKVPVQFMQALPYVLTVVLLAGFIGKAIPPRAGGVPYVKER"
     gene            complement(3358..4494)
                     /locus_tag="VGM46_03195"
     CDS             complement(3358..4494)
                     /locus_tag="VGM46_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009449217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4191626.1"
                     /translation="MSTPYAKLPRWADYGLIPLLNLLVAFIVAGFVVLLVGENPFRAA
                     VILVEGAFGRGQGIAFTLYYATTFIFTGLSVAVAVHCGLFNIGTEGQAYIGGLGVALV
                     CLAFDSVLPWWLSFPLAVLASAAVGALWALIPAYLQARRGSHIVITTIMFNFIAASIM
                     VYLLVGPLKPLTSQAPQTRLFLEGGQLPKLNWVIELFGAKVRSAPLNVSFLLALLMAF
                     LVWVLIWRTRLGYEMRTYGFSPKAARYAGISETRIIIVTMMISGALAGMMALNPTMGD
                     QHNVAIDFVGGAGFVGIAVALMGRSHPVGIVLAAILFGMLYQGGAELAFEMPAISRDM
                     IVIIQGLVILFAGALEHMFRPSIQALFASFSPKSVGMAAAGKGT"
     gene            complement(4499..4849)
                     /locus_tag="VGM46_03200"
     CDS             complement(4499..4849)
                     /locus_tag="VGM46_03200"
                     /inference="COORDINATES: protein motif:HMM:NF025625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HEY4191627.1"
                     /translation="MKLKVELGLDLAVSAELLSNERVRIYKGSEALRNRQKTTGQPMV
                     DRARLLRDDLIKAGVLVALVRDPDRLVFTQDIDFDTPTAAADIVFGANKSGREFWKSS
                     IDGKSLVELFGPAR"
     gene            complement(4846..6378)
                     /locus_tag="VGM46_03205"
     CDS             complement(4846..6378)
                     /locus_tag="VGM46_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017269602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4191628.1"
                     /translation="MAQAAIELIGINKSFGTVRANREINLEVARGAIHGIVGENGAGK
                     STLMSILYGFYQADSGEIRVNGQPATIKTSNDAIAAGIGMVHQHFMLVDNFTVLENII
                     LGAESDALLKKSIAKARSELGRLEREYGLEVDPDAIVEELPVGLQQRVEILKALYRGA
                     EILILDEPTGVLTPAEADHLFRILRQLKEQGKTVVLITHKLREIMAITDTVSVMRQGT
                     IVATRQTKATTVEELAELMVGRRVLLRVEKGEARPGAVKLAVADLTVKDARGVTMVND
                     VSFDVRAGEIVGIAGVAGNGQSELIEAICGIHRAVSGTVMLDGRPIDLTGAADPGELR
                     ERGLAHVPEDRHHVGLVLAFEENENSILGYHNDARYANGPFLNIEAIRANAEEKIEKY
                     DIRPSDCRLKTANFSGGNQQKIVLAREMEQDPGVLIVGQPTRGVDVGAIEFIHKRLIA
                     MRDAGKAVLVVSVELDEIRSLSDRILVMFAGRIVGERGPEAGEGELGLLMAGVERQEA
                     VQ"
     gene            6567..6776
                     /locus_tag="VGM46_03210"
     CDS             6567..6776
                     /locus_tag="VGM46_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010659381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HEY4191629.1"
                     /translation="MTTDPDRLTTLEIRAAEQEKSLEELSEQIASQWKVIERMQHKLN
                     TLTERFLALEEQSAPDVPITKPPHW"
     gene            6796..7188
                     /locus_tag="VGM46_03215"
     CDS             6796..7188
                     /locus_tag="VGM46_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191630.1"
                     /translation="MPRDNDRIARAGDYISGLMSDTERQRAERDLEVDPAFRDAVMQL
                     AERMRLFDGPSSPEDAVDDSWKLVAARIAELPQMQPMAIEANSTSRPEANDRVDRFAL
                     FSKKPAHALMIGIGVILAFALCYFTIKS"
BASE COUNT         1435 a         2443 c         2165 g         1206 t
ORIGIN      
        1 accgagtggt ctatccgctc gtccggcctg cggcggccgc cgccaagctc caccaccgca
       61 agcccgatat tgcgcgtttc aatgccggtg acgaaaccgc tttccgtcgc gcgcaccgca
      121 aattccaccg ccgcccgtgg cagatatttg tcggcccgtt cgataaaatc cgaaggtccg
      181 cccaaggcag ccaccatgcg cccgaacagc gccgccgcgc ggccggcttc cagcgcctcg
      241 cgggcgcggc gcagcccgtc ctgattggtc gaaaccaatt ccgccgccaa aagcatctcg
      301 gcggccagcg ccagcgtcac cgcctcaagt cgcgggtcgc gggttcgccc ggtcaggaaa
      361 tcgacggcgt tgcgtacctc aaccgcgttg cccgcggcgc aagccaatgg ctcgtccatg
      421 ccggtcacca gcgcggaggc cttcagcccc gcgccattgg caatctcgac cagactggcg
      481 gcaagcgcgg tcgcatcgcg cgatttctcc atgaatgcgc cattgccgac cttgacgtcg
      541 agcaccagcg agccaagccc ggctgccagc ttcttcgaca ggatggaggc ggtgatcagc
      601 ggcacggatt ccaccgtcgc cgtcacgtcc cgcaccgcat agagccgccg gtccgccggc
      661 gcgagatcga cggtctggcc gatgatggcg aaacctgttt cgagcacgac cttgcgaaac
      721 agcgtgaggt ccggctggct gacatagccg ggaatggcgt ccatcttgtc cagcgttccg
      781 ccggtatggc caagcccacg ccccgaaatc atcggcacat aggcgccgca actggcaacg
      841 atgggcgcca gcaccagcga gacattgtcg ccgacgccgc cggtcgagtg cttgtcggtg
      901 acgggtccgg gcagatgcga ccagttcagc acggaccctg agtcccgcat ggccagcgtc
      961 agcgccaccg cctcgtcgcg gctcatgccg ttgaagaaga cggccatggc gaaggccgcc
     1021 gcttgcccct ccgacaccga acccgaggtc aaaccggcga taaaggcgga aacttcctcg
     1081 ctcgacagcc gctggccgtc gcgttttctg cggatgattt cctgcgcaag catcttcaca
     1141 atgccccgga ttctcgcggc ctacccatcc agcaggcctt cccggaaaac atgccgcagg
     1201 atcaccgcca gccgcgcgcc gccgaccggc gccatgtcct tggtttcctg atgcgacagt
     1261 tccgccccgg tcatgccggc ggcgagattg gtgatgatcg agcacgcggc gacccgcagc
     1321 cccaggaagc gggcaagaat gacctccggc acggtcgaca tgccgaccgc gtccgcaccc
     1381 atcagccgcg ccatgcggat ttcagcgggc gtttcaaagg acggcccgga aaaccacata
     1441 tagacgccct tgtgcagcat cgtgccggtt gcctgcgcag cctgctcgaa tgcggtgcgc
     1501 atctgcgcgt catacgcttc ggtcagcccg acgaaacggc gctcgcctgg ctcgccgatc
     1561 agcggattga ggccggaaaa attgatgtgg tcttcgacca gcatgaccga gccgggcagc
     1621 atctgcggat cgagcgagcc ggcggaattg gtcacgatca gctttgcgat gcctattccc
     1681 gccaacgctt cgagcagcgg gcgcatcgcg gcagcgtcgc cctgctcgta ataatgggtg
     1741 cggcctgcca gcatcaggat cggcttgccg gcaaatcgcc ccgccaccat ttcgccggca
     1801 tggccactga cgctggctcg cgggaaaccc ggcatgtcgg cataggcgat ccgcaccgca
     1861 tcctcgacct gcccggccag cccgccaagg ccggagccga gcaccagcgc ggtggtcggg
     1921 gccagcccgt ccagcctttc gacaagcatg tcgatggcct gcttcatttc aggatgtcgc
     1981 ccttgaaacc atagggcagg atatcgccca tggtcacggt ttcgacgacg ccggtctcgt
     2041 cgcagagata gagcctggtt gcaggcccgc agaactccgc cagccgctgc cggcagccgc
     2101 cgcaaggcgt gcagcgcgcc atgcgctcgg cgacgacggc gatctccgtg atcttgccgc
     2161 ccccgcccat gacgtagtgg ccgagcgcgg tggtttccgc acaccaccct tcgggatagg
     2221 acgccacctc gatattggcc ccggcaaaga cttgtccgtc ttcggttcgc agcgccgcgc
     2281 ccacgggaaa tttcgaataa ggcgcatagg ctttcgccat ggccgcgcgg gcggcaaggt
     2341 ataggtcgtg cggcccagcg gtcatcagat cagcggtcat cggttcagcg ttccttcaca
     2401 tagggcacgc cgccggcgcg cggcggaatg gccttgccta tgaagccggc cagcagcacc
     2461 accgtcagca catagggcag cgcctgcatg aactgcaccg gcaccttgcc gatgagcggc
     2521 agcggcgttc cttgcagccg gatcgacagc gcgtcgagga acccgaacag caggcaggcg
     2581 aacatgacgt tcaccggctt ccatttggcg aagatcagcg cggccagcgc gatatagccc
     2641 ttgcccgccg tcatatcctt cacaaagccg ccattttgcg ccatcgacag gtaagcgccg
     2701 gccagcccgg tcaggatgcc ggtgcagatc agcgcgcgat aacgcagcca cgccaccgaa
     2761 atgcccgccg tatcgaccgc cgccggattt tcgccgacgg cgcgcagccg caggccaaag
     2821 cgggtgcgaa acagcaccca ccagcttacc ggcaccatca ggaaggcgag atagaccagg
     2881 atggaatggc ccgacagaag ctccgaatag agctgcccga tgaccggcac gtccttgacc
     2941 gcatcggcgc ccggcagcgt gatgggcgaa aagcgctcgc ccgcgcccag cgccggcgtc
     3001 cgcccgcctt gctggaacca ggcctgaccg aggatgatgg tggagccggc ggcgatgaag
     3061 tttatggcga cgcccgaaac gatctggttg ccgcgatgcg tgatcgaggc gaagccatgc
     3121 accagcgcga aacccaccga aattagaacg gccattccaa ggccgatgag cgcggaatga
     3181 aacaccgccg cggccgccgc ccccgcaaag gccccggcca gcatcttgcc ttccagcccg
     3241 atgtcgaaaa tgccggcgcg ctcggaataa agcccggcaa ggctggcaaa caacagcggc
     3301 accgaaagcc ggatcgtcga atcgagggtc tggacgatgg cgttgaacat atccatctca
     3361 ggtccccttg cccgctgcgg ccatgccgac cgatttcggg ctgaacgagg cgaacagcgc
     3421 ctggatcgac ggacggaaca tatgttccag cgcgcccgca aacaggatga ccagcccctg
     3481 gatgatgacg atcatgtcgc gcgagatcgc cggcatttcg aacgccagtt ccgcaccgcc
     3541 ctgatagagc atgccgaaca ggatggcagc caggacgatg ccgaccggat gcgagcgccc
     3601 catcagcgcc accgcgatgc cgacgaagcc cgcgccgccg acgaaatcga tcgccacatt
     3661 gtgctggtcg cccatcgtcg ggttgagcgc catcatgccg gccagcgcgc cggaaatcat
     3721 catggtgacg atgatgatgc gggtttcaga aataccggca taccgcgccg ccttgggcga
     3781 aaagccgtag gttcgcatct cgtagccgag ccgcgtgcgc cagatcagca cccagaccag
     3841 gaacgccatc aacagcgcca gcaggaacga aacgttcagc ggcgccgaac gaaccttcgc
     3901 cccgaacagt tcgatcaccc agttcagctt cggcaactgc ccgccttcaa ggaacagccg
     3961 cgtctgcggc gcctgcgagg tcagcggctt cagcggcccg accagcagat agaccatgat
     4021 cgaggctgcg atgaagttga acatgatggt ggtgatgacg atgtgcgagc cgcgcctggc
     4081 ttgcagatag gccgggatca gcgcccatag cgcgccgacc gccgccgaag ccaaaacggc
     4141 cagcggaaag ctcagccacc acggcaggac gctgtcgaag gcgaggcaca ccagcgccac
     4201 gccaaggccg ccaatatagg cctgcccctc ggtgccgata ttgaacaggc cgcaatgcac
     4261 cgccaccgcc accgaaagcc cggtgaagat gaaggtggtc gcataatata gggtgaatgc
     4321 gatgccctgc ccgcgcccga acgcgccctc gaccaggatg acggcggccc gaaacggatt
     4381 ctcgccgacc agcagcacga cgaagccggc aacgatgaaa gcaaccagca gattgagcag
     4441 cggtatcagc ccgtaatccg cccatcgcgg caatttggca tagggcgtgc tcattcgatc
     4501 accttgccgg accaaaaagc tctaccaaac tctttccgtc gatagatgat ttccaaaatt
     4561 cacgcccgct tttattagcg ccaaatacga tatcggcagc agccgtcgga gtatcaaaat
     4621 cgatatcctg cgtaaaaacc aggcggtcgg gatctcggac caatgcaaca agcaccccgg
     4681 ctttaattaa atcatcacga aggaggcgcg ctcgatccac catcggctgc ccagtagttt
     4741 tttgacggtt acgtagggcc tcggagcctt tgtagattct aactcgctcg tttgagagca
     4801 gctcggcgga aacagccaaa tccagaccaa gttcgacttt aagtttcatt gcaccgcctc
     4861 ctgccgctcg acgccggcca tcaacaggcc aagctcgccc tcgccggctt ccggtccgcg
     4921 ctcgccgacg atgcggccgg caaacatcac caggatacgg tcggacagcg agcggatttc
     4981 gtccagttcc accgaaacga ccagcaccgc cttgccggcg tcgcgcatgg cgatcagccg
     5041 cttgtggatg aattcgatgg cgccgacatc gacgccgcgc gtcggttggc cgacgatcag
     5101 cacgccggga tcctgctcca tctcgcgtgc cagcacgatc ttctgctggt tgccgccgga
     5161 aaaattggcg gtcttcagcc ggcagtcgga agggcggatg tcgtatttct cgatcttttc
     5221 ttcggcgtta gcgcggatgg cctcgatgtt caggaacggg ccgttcgcat agcgcgcatc
     5281 gttgtgatag ccgagaatgg aattctcgtt ctcctcgaag gccagcacca gcccgacatg
     5341 gtgtcggtct tccggcacat gcgccagccc gcgttcgcgc aactcgccgg ggtcggccgc
     5401 cccggtcaga tcgatcggcc tgccgtccag catgacggtg ccggaaacgg cgcggtgaat
     5461 gccgcagatc gcctcgatca gttccgactg gccgttaccg gcgacgccgg cgatgccgac
     5521 gatctcgccg gcgcgcacgt cgaaggaaac gtcgttcacc atggtgacgc cgcgcgcgtc
     5581 cttcaccgtc aggtcggcga cggccagctt gactgcaccc ggcctcgcct cgcctttctc
     5641 gacgcgcagc agaacgcgcc gcccgaccat cagctctgcc agttcttcca ccgtggtcgc
     5701 cttggtctgg cgtgtggcga cgatggttcc ctgccgcatc accgacaccg tatcggtgat
     5761 cgccatgatc tcgcgcagct tgtgcgtgat cagcaccacc gtcttgccct gctccttcaa
     5821 ctgccgaagg atgcggaaaa ggtggtccgc ctcggccggc gtcagcacgc cggtcggctc
     5881 atcgagaatg aggatttccg cgccgcgata cagcgccttg agaatttcca cccgctgctg
     5941 aaggccgacc ggcagttcct cgacaatggc gtcggggtcg acttccagcc cgtattcgcg
     6001 ttccagccgc ccgagttcgg acctggcctt ggcgatgctt ttcttcagca gcgcatcgct
     6061 ttcggccccg aggatgatgt tttccagcac ggtgaaatta tcgaccagca tgaaatgctg
     6121 gtgcaccatg ccgatgccgg cggcgatggc gtcgttggaa gtcttgatgg tggccggttg
     6181 gccgttgacg cgtatttcgc cgctgtcggc ttggtagaaa ccgtagagga tcgacatcag
     6241 cgtcgatttg ccggcgccgt tctccccgac gatcccgtgg atggcgcccc gcgcgacctc
     6301 aagattgatc tcgcggttgg cccgaaccgt gccgaagctt ttgttgatgc cgatcagctc
     6361 gattgcggct tgcgccattc agcagttcca cccttcagtc ttgcctaaca cgggcgcccg
     6421 cgccctgcca attgagcgcc gacaaaaccc actctcgaca aacgcccgcg cgcttgtcaa
     6481 tttcgaacgc ggctgaaatc atcactttcg caaaccgcag cttgaacaac caatgctcgc
     6541 ctgagcgcat gcagggaaat tccttaatga cgaccgaccc ggaccggctg acgacattgg
     6601 aaattcgtgc cgccgagcag gaaaaatccc ttgaggaatt gtccgaacag atcgccagcc
     6661 agtggaaagt gatcgagcgc atgcagcaca agctcaacac gctgaccgag cggttcctgg
     6721 cgctggagga gcaatccgcc cccgacgtgc ccattaccaa accaccgcac tggtgattgc
     6781 cgggagaaaa gcgccatgcc tcgtgacaac gaccgaattg cacgggctgg cgactacata
     6841 tcgggcttga tgagcgacac cgagcgccaa cgggccgaac gcgaccttga ggtcgatccg
     6901 gcctttcgcg atgcggtgat gcagttggcc gagcggatgc gcctcttcga tggcccatcc
     6961 tcgcccgagg acgctgtgga cgatagctgg aagctggttg ccgcccgcat cgccgaactg
     7021 ccgcagatgc agcccatggc gatcgaggcc aacagcacca gccgcccgga agccaacgac
     7081 cgggtcgacc gttttgcgct gttttccaag aagccggcac acgccctgat gataggaatc
     7141 ggcgtaatac tggctttcgc gctgtgctac ttcacgatca aatcctaaac gcaggcaggt
     7201 cctgagcggc gggcgcgagc cggcgcatca tcaaaaacat cggttcgcc
//
LOCUS       DASYCS010000092         2780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_453226,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000092 DASYCS010000000
VERSION     DASYCS010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453226"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="VGM46_03220"
     CDS             complement(<1..777)
                     /locus_tag="VGM46_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010914430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEY4191631.1"
                     /translation="MADKSEKMKARQPRGFVDRSAEDIRAVDRMMAKIREVYELYGFE
                     PADQPLIEYTDALGKFLPDQDRPNAGVFSFRDDEGQEAAEGDTAGKQGEWLSLRYDLT
                     APMARFVAENYDRLPKPFRSYRAGWVFRNEKPGPGRFRQFMQFDADTVGTPGVAADAE
                     MAMMMADVMEALGIARGDYVIRVNNRKVLDGVLEAIGLGGDDNAGRRLTVLRAIDKLD
                     KVGAEGVRDLLGKGRMDESGDFTKGAGLDDKQMERLGGSFM"
     gene            complement(880..1518)
                     /locus_tag="VGM46_03225"
     CDS             complement(880..1518)
                     /locus_tag="VGM46_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEY4191632.1"
                     /translation="MADENTGLLAGPDGAKRCFWHGNLPDYLHYHDHEWGRPVADDTR
                     LFEKICLEGFQSGLSWLTILRKRENFREAFDGFDFDRVAKFTDADVERLVGNAGIIRH
                     RGKIVSTINNAKRAREMADEAGSLGAWFWRFEPGPQERPEVMDLAHLRANPTTAVSVR
                     LSKELKKRGWSFVGPTTVYAFMQAMGLVNDHLEGCVCRDEVEVLRKAFKRPA"
     gene            1736..2497
                     /locus_tag="VGM46_03230"
     CDS             1736..2497
                     /locus_tag="VGM46_03230"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006329427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEY4191633.1"
                     /translation="MKLLLLPMLGAAMLALSATASIADDRYAERPPVTVSPDLSAPWV
                     IQLGGQPEAAARSRETPQPARRQRREVQPIDQVQVQPDRVRTAAVQAPARKLAGHRPM
                     DPIYLPQEVAYQGGQKPGTIIIDTTKNFLYLVQKDGKARRYGVGTGKPGFEWAGTHKI
                     TQKREWPDWRPPAEMIAREAARGRSLPAHMEGGIENPLGARAMYLGSTLYRIHGTNQP
                     WTIGGSVSSGCIRMRNQDVVDLYERVPVGTSVVVM"
BASE COUNT          508 a          903 c          858 g          511 t
ORIGIN      
        1 cataaacgac ccacctagcc gctccatctg cttgtcatcc agcccggcgc ccttggtaaa
       61 atcgccgctc tcgtccatgc ggcccttgcc gagcagatcg cgcacgcctt ccgcgccgac
      121 cttatctagc ttgtcgatgg cgcgcagcac ggtcaggcgg cggccagcat tgtcgtcgcc
      181 gccaaggccg atggcctcaa gcacaccgtc cagcaccttg cggttgttga cgcggatgac
      241 gtagtcgccg cgcgcaatgc ccagcgcttc catcacgtca gccatcatca tcgccatttc
      301 ggcgtccgcc gcgacgcccg gcgtgccgac cgtgtcggca tcgaactgca tgaactggcg
      361 gaagcggccc ggaccgggct tctcgttgcg gaacacccag ccggcgcgat aggaacggaa
      421 gggttttggc aaacggtcgt agttttcggc gacgaagcgc gccatcggtg cggtcaggtc
      481 atagcgcagc gacagccact ctccctgttt tccagccgta tcgccttccg ccgcctcttg
      541 gccctcatcg tcccggaacg aaaaaacgcc ggcgttcggg cggtcctggt cgggcaggaa
      601 tttgcccagc gcgtcggtat attcgatcaa gggctggtcg gccggctcga agccgtaaag
      661 ctcgtagacc tcgcggattt tcgccatcat ccggtcgacg gcgcggatat cctcggcgct
      721 gcggtccacg aagccgcgcg gctggcgcgc cttcatcttt tccgatttgt cggccatggt
      781 atccgtcgct tttcaggctg ctgccgggct aaaatatcta tgccgcgcgt cctaaaccag
      841 gaaggccggg gcggcaaggg tttggcggcg atgaaccggc tatgcgggcc gcttgaacgc
      901 cttgcgcagc acttccacct catcgcggca gacgcagcct tcgagatgat cattgaccag
      961 ccccatcgcc tgcatgaagg cataaaccgt ggtcgggccg acaaagctcc agccgcgctt
     1021 tttcagctcc ttcgacagcc gcaccgatac cgccgtggtc gggttggcgc gcagatgagc
     1081 gagatccatc acttccgggc gttcctgtgg ccccggctcg aaccgccaga accacgcacc
     1141 gagcgagccg gcctcgtcgg ccatttcgcg ggcgcgtttg gcgttgttga tggtggaaac
     1201 gatcttgccg cgatggcgga tgatgccggc attgccgacg aggcgctcga catcggcatc
     1261 ggtaaacttg gccacccggt cgaaatcgaa gccgtcaaag gcttcgcgga aattctcgcg
     1321 cttgcgcagg atggtcagcc acgacaggcc ggactggaag ccttcgaggc aaatcttttc
     1381 gaacagcctg gtgtcgtcgg ccacgggccg gccccattca tggtcgtgat aatgcaggta
     1441 atccggcaga ttgccgtgcc agaagcagcg cttcgcgcca tcggggccgg cgagaaggcc
     1501 agtgttttcg tctgccatgg tgcaaaatcc caatcgttaa tgtggcgcgc agccgcttta
     1561 ccggggcttt accagattcc tgaacccgtc ggtaaccaca ccggaaagtt tactgacaat
     1621 atcgcatttt aagtatttcg attcacgttt cggtcgcagc tgcggaccga tgatcgtgca
     1681 aaccgggcgg gaagccaccc ggttcctgtg acgatcagtt cgagagtgcg tcccgatgaa
     1741 actgcttcta ctgcccatgc ttggcgcggc aatgcttgcc ttaagcgcca ccgcctccat
     1801 cgccgacgac cgctacgcgg agcggccgcc ggtaacggtc agcccggacc tgtcggcgcc
     1861 gtgggtcatc cagcttggcg ggcagccgga agccgcagcg cggagccgtg aaacgccaca
     1921 gccggcgcgc cggcaacggc gtgaagtgca gcccatcgac caggttcagg tgcagcccga
     1981 ccgcgtgcgc accgcagccg tgcaggcgcc ggcccgcaag ctcgccggcc accggccgat
     2041 ggacccgata tatttgccgc aggaagtcgc ctatcagggt gggcagaagc ccggcaccat
     2101 catcatcgat acgaccaaga atttcctcta tctcgtccag aaggacggca aggcgcggcg
     2161 ctacggcgtc ggcaccggca agcccggctt cgaatgggcc ggcacccaca agatcaccca
     2221 gaagcgcgaa tggcccgatt ggcggccgcc agccgaaatg atcgcgcgcg aagcagccag
     2281 agggcgctcc ctgccggcgc acatggaagg cggcatcgaa aacccgcttg gcgcgcgggc
     2341 catgtatctc ggatcgacgc tttaccgcat ccacggcacc aaccagccat ggactatcgg
     2401 cggctcggtg tcttcgggct gcatccggat gcgaaaccag gacgtggtcg atctctacga
     2461 gcgcgtgccg gttggcacca gcgtcgtggt gatgtagcgt ttcaaggcaa taacggggga
     2521 cgggccgtgt ggggacatgg ccggtcgaaa aaaagggcag cgccgcttcg gttgcgctgc
     2581 cctttttcgt tgtccggggt tggctacggg tcggggtcct tgccgcgtac tcgcctaaac
     2641 atttcgaaag ccgcctttcg gtcgcatcga gagtaacgcg gttcgctttc acgagggtaa
     2701 tggctccgcg ctcctgctac gccattgctg aaaccacagg cgaaggcttt gccatgtccg
     2761 acgatacgcg cgacctcaaa
//
LOCUS       DASYCS010000093        16133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4513223,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000093 DASYCS010000000
VERSION     DASYCS010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 16133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16133
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4513223"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="VGM46_03235"
     CDS             complement(<1..814)
                     /locus_tag="VGM46_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HEY4191634.1"
                     /translation="MADLSSASFVGVDWGTSSFRLWVMGGDGAPLAASRSGEGMLSCQ
                     KSGFQPVLDRHLAEVGAPDGLPVLICGMAGARQGWVEAAYVHAPTRLGELHERAIRVP
                     VAGRDIRILPGIAQMEADAPNVMRGEETQLLGSITDDFTGLVCMPGTHCKWVSVRQGA
                     VTGFATFMTGELFSLIGAQSVLAHSVEAGAAPLASDPGFRAALVSVLDDAGRALSGLF
                     SIRAAQLLGFEEKRQGAPRLSGLLIGSEIAAARSLYGAAGEIVLLASGALGNL"
     gene            complement(826..1746)
                     /locus_tag="VGM46_03240"
     CDS             complement(826..1746)
                     /locus_tag="VGM46_03240"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY4191635.1"
                     /translation="MSGYRIVEHEVACVVDSHDKLGEGAFWCPDEGALYWVDIAMPSA
                     LHRLTPATGRHDSWPMPEMISALAKRGDGTLLVASQSGLGFFDPARPQLQRVAAPEAH
                     KPANRSNDGAPDASGRFWIGTMQNNFAPDGSEIPVESATGSLWRIEAHLKATEVISGI
                     GITNGVAWSPDSRTLYVVDSMTQTIFAYDFDLEPGTVGNRRVFSDVKDMGDPDGNAVD
                     AQGYLWSARWEGHGLMRFAPDGTVDRVVPIPASRVTSCAFGGPDLATLYVTTSRRGVD
                     AETLARYPQQGGLFSLRPGVSGLPRAKFAG"
     gene            complement(1832..2560)
                     /locus_tag="VGM46_03245"
     CDS             complement(1832..2560)
                     /locus_tag="VGM46_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011975570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEY4191636.1"
                     /translation="MLKAAISGGRIRTSHAFVVDGIGKAIVSGEYGQGSILPGDAELE
                     ARFNVSRTVVREAMKTLAAKGMIVARARIGTRVTARSSWNYFDSDILRWHLENGADVD
                     FLSHLAEMRLSFEPYAAQLACMRADPAHIARMHEQADAMTVATSMEAFALADLDFHMA
                     LLEASKNPFMYSVGALTEAALVTSFRVSSPHGDAARQAKAAGEHRRIVEAIEAKNQQE
                     AADAVVWVILEGKERIVQTLKGSH"
     gene            complement(2565..3542)
                     /gene="yjfF"
                     /locus_tag="VGM46_03250"
     CDS             complement(2565..3542)
                     /gene="yjfF"
                     /locus_tag="VGM46_03250"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019963730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HEY4191637.1"
                     /translation="MNARLLPFFATVLIFILAYAVCYMQFPAMLSTRVAGNLLTDNAF
                     LGIVAVGMTFVILSGGIDLSVGSVIAFSGVFIAVVLRDTGLHPLVVFVLLLAITTLFG
                     AAMGLIIHVLQMPPFIVTLAGMFLARGMAYVLSVDSVPIAHPFYEWAQNAYWLMPGKG
                     RLTLIGAMMLVVVLAGVVLAHRTRFGANVFALGGGVQTARLMGVPIARTTILIYALSG
                     FLAGLSGIVFSFYTSAGYSLATVGVELDAIAAVVIGGTLLSGGSGFVAGTLVGVLIMG
                     LIQTYITFDGTLSSWWTKIVIGALLLGFIMLQKLLVGLTGRRMRAPGAH"
     gene            complement(3539..4540)
                     /locus_tag="VGM46_03255"
     CDS             complement(3539..4540)
                     /locus_tag="VGM46_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007532656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4191638.1"
                     /translation="MKGGAVRFLRRIAPQLVTLAVVLAMIGMVFPGFFEISMANGRLY
                     GSPIDILNRGAPVALLAIGMTLVIATGGIDLSVGAVMAICGAVSAAAITAGYGLAATL
                     ALALGAGLACGLWNGLLVAVLGIQPIVATLILMVAGRGIAQLITEGTILTFSDEGLAF
                     LGSGTLLGLPTPVVIWLAMGALIVLLVRRTALGMLIEAVGVNRRASMLAGVNARLLVV
                     TVYMVSGLCAALAGIIVTADIRGADANNAGLFLELDAILAVVIGGNSLLGGRFSLVAA
                     LFGAVIIQAVNTGILVAGFPPEFNLVIKACIVLAILLVQSPALQGSRLLLRKGARLS"
     gene            complement(4537..6078)
                     /locus_tag="VGM46_03260"
     CDS             complement(4537..6078)
                     /locus_tag="VGM46_03260"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014530353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEY4191639.1"
                     /translation="MTPGEGQTLLSARALTKSFPGTKALDGVDFDLRAGEVHALLGEN
                     GAGKSTLIKCLTGAIRRDGGDIVLDGRSIDPRDTLHAQQLGIGTVYQEVNLLPNLTVA
                     ENLMLGRQPCRFGLVSRKAMNSQAREMLAQYGLSLDVTRNLDSYSVAIQQIVAIARAV
                     SLSGSVLILDEPTASLDRDEVRMLFDVVRGLREKGLGIVFISHFLDQVFEISDRITVL
                     RNGKLVGVRDVAALDRLQLVNLMLGRELAEETRHHGRTGIDYSTEPRLRFSGTGRRGA
                     ISPFDLAIQRGEVIGVAGLLGSGRTETVELMFGAAPMDSGTVSMDGKALRLASPRDAV
                     AAGFGFSPEDRKTSGIIGDMGVRENIALALQARTGWMRPVSLRRQRELAQDYIRRLDI
                     RTSDMEKPVGQLSGGNQQKVLLARWLATNPQFLILDEPTRGIDVGAHAEIIRMIEELC
                     TGGMSLLVISSELDELVAYSHRVVVVRDRAHVAELMGEAITSESIVQAIATQHGATAT
                     GQAAS"
     gene            complement(6152..7111)
                     /gene="ytfQ"
                     /locus_tag="VGM46_03265"
     CDS             complement(6152..7111)
                     /gene="ytfQ"
                     /locus_tag="VGM46_03265"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019995881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter,
                     galactofuranose-binding protein YtfQ"
                     /protein_id="HEY4191640.1"
                     /translation="MTARKLLGASLLASITLFTSAALAETVGFAQIGSESGWRAAETT
                     LTKQEAEKRGIDLKFADAQQKQENQIKAIRSFVAQGVDAILLAPVVATGWDAVLKEAK
                     EANIPVVLLDRMVDSSPDLYLTAIGSDLVHEGKVAGQWLVDDEGSKECNIVELQGTTG
                     SSPAIDRKKGFEEAIAGHANLKIIRSQTGDFTRAKGKEVMESFLKAEDGGKNICALYA
                     HNDDMAIGAIQAIKEAGLKPGTDIKVISVDSIPDIFQAMAAGEANATVELTPNMAGPA
                     FDALKAYKDNGTVPPKFIQTESKLYTQKDDPKAVYELKKGLGY"
     gene            7331..8581
                     /locus_tag="VGM46_03270"
     CDS             7331..8581
                     /locus_tag="VGM46_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006205375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HEY4191641.1"
                     /translation="MISAKTAADTFNHPVRAMAGRDPHETHRVSTPLELLFDLTFATA
                     FGFAASQLAHALAAGHYTAALIGFGFATFAICWAWINFSWFASAYDTDDWVFRVVTMV
                     QMTGVLVLAIGLPRMFASVEHGEYLDNSVMVLGYVIMRLAMVFQWLRAARQDPVRHRV
                     CLTYAIAISIAQLGWLMQIFSSFSVGASIILGGILALIELSGPVIAERKDGGTPWHAH
                     HIAERYSLFAIIALGEGIIGTVAALSAAVEEQGWSLDAALVCIAGVGLTFGIWWVYHL
                     LPSAQVLHANRTRSFIWGYGQMVIIAAIVATGAGLHVAAYFIEHKAAIGPLATVLCVA
                     VPVAVFLVGVYALYYHLVRHFDRLHTWLLLGTAAVVVLAVLAALAGVDMAVCLVILML
                     APAVSVVGYEMQGHHHTEGVLAAK"
     gene            complement(8587..9531)
                     /locus_tag="VGM46_03275"
     CDS             complement(8587..9531)
                     /locus_tag="VGM46_03275"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY4191642.1"
                     /translation="MQESGSDRLLRRGLKLSHLRLLGTLAKTGQISAAAASLGISQPG
                     ASRLLAEAERISGTALYRRHARGVEFTEAGEFFATLARRMLGDLDAAAREIEDMDAGR
                     RGFVAIGAVTGAAMEHVLPVLRQIRVTHPRINVHVVVDTSDRLAPLMLTDELDLYIGR
                     VPVDVDRNAFIADPIGPEPVGLIVRQGHPLMRRDSPSLAECVEFDWVLQPLGGLMRQT
                     VETYLMERGIALPARVISTSSMLMTLALIAQSNAISPVARAAATFFGSGEGLGSVIAT
                     LPVADDLVVAPYSLLRPAHRPLTPASRVVHEQIRSRLR"
     gene            9668..10735
                     /gene="chvE"
                     /locus_tag="VGM46_03280"
     CDS             9668..10735
                     /gene="chvE"
                     /locus_tag="VGM46_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY4191643.1"
                     /translation="MRSLLLALGTAALGALLISSSAQAQDKGLVGIAMPTKSSLRWIN
                     DGDSLKKSLEEKGYTVDLQYAEDDIPNQLAQIENMVTKDAKALVVASIDGTTLSAVLK
                     QAADKGVKVIAYDRLIRDSANVDYYTTFDNFKVGVLQADSLLKGLGYPEKKGPFNIEL
                     FGGSPDDNNAFFFYDGAMSVLQPLIDKGDLVVKSGQMGMEKVGTLRWDAAVAQARMDN
                     ILSANYSDGSRVDAVLAPYDGLSRGIISSLRGVGYGTASQPWPIISGQDAETPSIKAI
                     VAGEQYSTVFKDTRELAKVTANMVDAVLSGGKPEINDEKTYNNGVKVVPSYLLVPVAV
                     DKSNYEKIVVDSGYIKAEDLK"
     gene            10851..12404
                     /gene="mmsA"
                     /locus_tag="VGM46_03285"
     CDS             10851..12404
                     /gene="mmsA"
                     /locus_tag="VGM46_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEY4191644.1"
                     /translation="MSDVILEMRGITKTFPGVKALENVNLKVKRGDIHAICGENGAGK
                     STLMKVLSGVYPYGSYSGDIQYEGKECRFNGINDSEKLGIVIIHQELALVPLLSIGEN
                     IFLGNEQAKNGVIDWNRVRRRSAELLKKVGLRESPDTLITNIGTGKQQLVEIAKALSK
                     DVKLLILDEPTASLSEKDSQSLLDLLLEFKAQGMTSIMISHKLNEIKRIADQITVIRD
                     GQSIETMDQIDITEDRIVTSMVGRSMENRYPARTPNIGETVFEVKNWSVYHPIHPERQ
                     VIKGINLNVRRGEVVGIAGLMGAGRTEFAMSVFGKTYGANISGEVLLHGKPADTSSVS
                     KAVAAGLAYATEDRKTYGLNLIDHIKHNVTIANLPGISSSGVIDDTAEVKVANEYRRK
                     TNIRSSSVYQIAGNLSGGNQQKVVLSKWLFANPDVLILDEPTRGIDVGAKYEIYTIIN
                     KLAEEGKGILVISSEMPELIGICDRIYVLNEGRIVGEMAGEEATQEKIMRAIMRGEAR
                     EEQMERAAS"
     gene            12401..13657
                     /gene="mmsB"
                     /locus_tag="VGM46_03290"
     CDS             12401..13657
                     /gene="mmsB"
                     /locus_tag="VGM46_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018429233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HEY4191645.1"
                     /translation="MSTEVNAPATSAAGASAHTAIKDNLREYGLVIALIAIMLFFQFM
                     TNGVLFRPVNLTNVILQNSYIVVMALGMLLVIVAGHIDLSVGAVAGFIGAVAAILMVD
                     YGMNPLLAGFLCILAGAVVGGIQGYFIAYMKIPSFIVTLAGMLVFKGLLLNVLSGRSV
                     GPFPEVFQLLSAGFIPDVIGPMTIIAPTLNASGAPTPGTGIVLHSTTMLIGVLIVAAM
                     LYFALKSRRNRESHGYDAEPFAFFVAKNLVIAGIVLFLMFKFASYRGLPNVLMVMGVL
                     IALFVFVTTRMTFGRRIYAMGGNIKAAALSGIQTERLTFYIFVIMGMLAALAGLIYSA
                     RLNMATPKAGAGLELDVIAAVFVGGASALGGVGQVTGAVIGAFIMGVMNNGMGVMGIN
                     IDWQQVIKGLVLLGAVAFDLYNKKKA"
     gene            13663..14667
                     /gene="araD1"
                     /locus_tag="VGM46_03295"
     CDS             13663..14667
                     /gene="araD1"
                     /locus_tag="VGM46_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015319285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraD1 family protein"
                     /protein_id="HEY4191646.1"
                     /translation="MLLSQIGNPDGSITVVAREGKEAYAVKGADSLYAVVLDCIRSGS
                     VDLAAHIAALGLGSALDLERAYEEDRLLPPLTHPDPAHLHLTGTGLTHLGSAATRDAM
                     HKKAGAETEEKLTDSMKMFRLGLEGGKPANGNAGVQPEWFYKGNGTSLAAPGGTLASP
                     AFAEDSGEEPEIAGIYVIGEDGTPFRVGFALSNEFSDHVMERRNYLYLAHSKLRPASF
                     GPEIRTGALPADIRGTSRIRRGKKVIFEKPFLSGEANMSHSIANLEHHHFKYGVFRQP
                     GDVHVHMFGTATLSFADGISTEAGDQFEIEAAPFGMPLRNRLAVETAGACARVNVRAL
                     "
     gene            14683..>16133
                     /locus_tag="VGM46_03300"
     CDS             14683..>16133
                     /locus_tag="VGM46_03300"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006939359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HEY4191647.1"
                     /translation="MPFRKAEWPRKLRSHEWYGGSSRDNIYHRSWMKNQGLPADLFDG
                     RPVIGICNTWSELTPCNGHLRDLAEKVKFGIYEAGGLPVEFPVFSTGESSLRPTAMMY
                     RNLAAMDVEEALRANPLDGVVLLVGCDKTTPALLMGAASVDIPAIAVSGGPMLNGWFR
                     GERVGSGTALWQMSEDIKAGKMTREDFLEAEQAMSRSPGSCNTMGTASTMASMAEALG
                     MALSGNAAIPAVDSRRRVMAHLTGRRIVQMVKDDLKPSDILTRQAFENAIRVNGAIGG
                     STNAVIHLLAIAGRIGVDLTLDDWDRLGRDVPTVVNLMPSGKYLMEEFFYAGGLPVVI
                     KRLGEAGMLHGDALTVSGEAIWDEVKDAVNWNEDVILPVERALTSHGGVAVLKGNLAP
                     KGAVLKPSAASPHLMQHRGRAVVFEDIDDYKRKIDDEALDIDESCIMVMKNCGPRGYP
                     GMAEVGNMGLPPKVLRKGITDMVRISDARMSGTA"
BASE COUNT         2971 a         5263 c         5136 g         2763 t
ORIGIN      
        1 agagattgcc gagcgcgccc gacgccagca gcacgatttc gcccgctgcg ccgtaaagcg
       61 agcgcgccgc cgcgatctcc gaaccgatca aaaggccgga caggcgcggt gcgccctgcc
      121 gcttttcctc gaagccgagc agttgcgcgg cgcggatgga aaacaggcct gacagcgccc
      181 tgccggcatc atccagcacc gaaaccagcg ccgcgcgaaa gccgggatcg ctggcaagcg
      241 gcgccgcgcc cgcctccacc gaatgcgcca gcaccgattg cgcgccgatg agcgagaaca
      301 gttcgccggt catgaaggtg gcaaagccgg taacggcccc ttgcctgacc gaaacccact
      361 tgcaatgggt gccgggcatg cagaccagcc cggtgaaatc atcggtgatg gaaccgagca
      421 actgcgtttc ctcgccgcgc atgacatttg gcgcatcggc ctccatttgc gcgatgccgg
      481 gcaggatgcg gatatcgcgc cctgccaccg gtacgcggat ggcgcgctcg tgcaactcgc
      541 cgagccgcgt cggcgcatgc acataggctg cctcgaccca gccttgacgc gcgccggcca
      601 tgccgcagat gaggacgggc aggccatccg gcgcgccgac ttcggccagg tgacggtcga
      661 gcaccggctg gaagcctgat ttttggcagg aaagcatgcc ctcgccgctg cggctggcgg
      721 ccagcggcgc gccatcgccg cccatcaccc acagccggaa actgctggtg ccccaatcga
      781 cgccgacgaa ggatgcgctg gaaagatcag ccatgctttt cccaatcaac ccgcaaactt
      841 cgcacgcggc aggcccgata cgccggggcg cagcgaaaac aggccgccct gctgcggata
      901 gcgcgccagc gtctccgcat cgacgccccg ccgcgatgtc gtcacgtaaa gcgtggcgag
      961 gtcggggccg ccaaaggcgc aggaggtgac gcgcgaggcc gggatcggca ccacgcggtc
     1021 gaccgtcccg tccggcgcga accgcatcag gccgtggccc tcccagcgcg cgctccacag
     1081 atagccttgc gcatccaccg cattgccgtc cgggtcgccc atgtccttca cgtcgctgaa
     1141 cacgcgccgg ttgccgaccg tgccgggctc caggtcgaaa tcgtaggcga agatcgtctg
     1201 cgtcattgaa tcgacgacat agagcgtgcg ggaatccggg ctccaggcga cgccgttggt
     1261 gatgccgatg ccgcttatca cctcggtggc cttgaggtgg gcctcgatcc gccatagcga
     1321 tccagttgcg ctttccaccg gaatttcgga accgtccggc gcgaaattgt tctgcatggt
     1381 gccgatccag aaccggccgg aagcatccgg tgcgccatca ttgctgcggt tggccggctt
     1441 gtgcgcctcg ggcgcggcga cgcgctgcaa ttgcggtctg gccggatcga agaagccgag
     1501 gccgctttgc gaggcgacca gcagcgtgcc gtcgccgcgc tttgccagcg cgctgatcat
     1561 ttccggcatc ggccagctgt cgtggcggcc ggttgccggc gtcaggcggt gcagggcgga
     1621 aggcatggcg atatccaccc aatagagcgc gccttcgtcg ggacaccaga aagcgccctc
     1681 gcccagcttg tcatggctgt cgaccacgca ggcgacttca tgttcgacga tccgatatcc
     1741 gctcatctgc cctgctcttc ctgtcgaaaa tcacttgaat ggcgcagccc cgccttcggg
     1801 caacgccggt tttccttacg tcttttgcgc ctcagtgcga ccctttcagg gtctggacga
     1861 tacgctcctt gccctccagg atgacccaga ccaccgcatc ggcggcttcc tgctggttct
     1921 tcgcctcgat ggcctcgacg atccgccggt gttcgccggc ggccttggct tgcctggccg
     1981 catcgccatg gggcgagctt acgcgaaagc ttgtcaccag cgcggcttcc gtcagcgcgc
     2041 cgaccgaata catgaacggg tttttcgacg cttcaagcag cgccatgtgg aaatcgagat
     2101 cggccagcgc gaacgcctcc atcgaggtcg ccaccgtcat cgcgtcggcc tgctcgtgca
     2161 tgcgggcgat atgggcgggg tcggcgcgca tgcaggccag ctgcgccgca tagggttcga
     2221 acgaaaggcg catctcggct agatggctca ggaaatcgac atcggccccg ttttcgaggt
     2281 gccagcgcag aatgtcggaa tcgaaatagt tccagctcga acgcgccgtg acgcgggtgc
     2341 cgatgcgggc gcgcgccacg atcatgccct tggcggccag cgtcttcatc gcctcgcgca
     2401 cgacagtgcg cgaaacgttg aaccgcgcct caagctcggc gtcgcccggc aggatggagc
     2461 cttgcccgta ttcgcccgaa acgatcgcct tgccgatgcc gtcaaccacg aaagcatggc
     2521 tggtgcggat gcgccctccg gaaatcgcgg ccttcagcat ggcctcaatg cgctccgggc
     2581 gcgcgcatgc gacggccggt caggccgacc agcagctttt gcagcatgat gaagccgagc
     2641 agcagcgcgc cgatgacgat cttcgtccac cagctggaaa gcgtgccgtc gaaggtgatg
     2701 taggtctgga tcaggcccat gatgagcacg ccgacgagcg tgccggcgac gaagccgctg
     2761 ccgccgctaa gcaaggtgcc gccgatgacg acggcggcga tggcgtccag ctcgacgccg
     2821 actgtcgcca gcgaatagcc ggccgacgta tagaatgaaa agacaattcc cgataaccct
     2881 gccaagaacc ccgacaaagc atagatgagg atggtggtgc gggcaatcgg aacccccatc
     2941 agccgggccg tctgcacgcc gccgccgagc gcgaagacat tggcgccgaa acgggtgcgg
     3001 tgcgccagga cgacgccggc gagcaccacc accagcatca tcgcgccgat cagcgtcagc
     3061 ctgcctttgc cgggcatcag ccagtaggcg ttctgcgccc actcgtagaa ggggtgcgcg
     3121 atgggcacgg aatccaccga cagcacatag gccatgccgc gcgccaggaa catgccggcc
     3181 agcgtgacga tgaagggcgg catttgcagc acatggatga tcagccccat ggccgcgccg
     3241 aacagcgtgg tgatggccag cagcagcacg aagacgacga gcggatgcag cccggtatcg
     3301 cgcagcacca cggcgatgaa gacgccggag aaggcgatga ccgagccgac cgacaggtcg
     3361 atgccgcccg acaggataac gaaggtcatg ccgaccgcga cgatccccag aaaggcgttg
     3421 tcggtcaaca gattgccggc gacgcgggtg gaaagcatgg ccgggaactg catgtagcag
     3481 accgcatagg ccaggatgaa gatgaggacg gtggcgaaga aaggcaaaag gcgagcgttc
     3541 atgaaagcct ggctcccttg cgcaacaaca ggcggctgcc ttgcagggcc ggcgactgca
     3601 cgaggaggat ggccagcacg atgcaggcct tgatgacgag gttgaattcc ggcgggaagc
     3661 cggcgacgag gatgccggtg ttgaccgcct ggatgatgac cgcgccgaac agcgcggcga
     3721 ccagcgagaa gcgcccgcca agcagcgaat tgccgccgat gacgacggcc agaatggcgt
     3781 ccagttcgag gaacagcccg gcattgttgg cgtcggcgcc gcgaatgtcg gcggtgacga
     3841 tgatcccggc caacgccgcg cacaggccgg agaccatgta gacggtgacc accagcaggc
     3901 gggcattgac gccggccagc atgctggcgc gccggttgac gccgaccgcc tcgatcagca
     3961 tgccaagtgc ggtgcgccgc accagaagca cgatcagcgc gcccatcgcc agccagatga
     4021 ccaccggcgt cggcaggcca agcagcgtac ccgaaccgag aaaggcgagg ccttcatcgc
     4081 tgaaggtgag gatggtgcct tcggtgatga gctgcgcgat gccgcgcccg gccaccatca
     4141 ggatcagcgt ggcgacaatg ggctggatgc cgagtacggc gaccagcagg ccgttccaca
     4201 ggccgcaggc aaggccggcg cccagcgcca gcgccagcgt ggcggccagg ccgtagccgg
     4261 cggtgatggc ggcagccgaa accgcgccgc aaatggccat gaccgcgccg accgacaggt
     4321 cgatgccgcc ggtggcgatg accagcgtca tgccgatggc cagcagcgcc actggcgcac
     4381 cccggttcaa aatgtcgatc gggctgccat aaaggcggcc attggccatc gatatctcga
     4441 agaatccggg gaaaaccatg ccgatcatcg ccagtacgac cgccagcgtg accagttgcg
     4501 gcgcgatgcg ccggaggaaa cggaccgccc cgcccttcac gacgcagcct gcccggttgc
     4561 ggtggcgcca tgctgggtgg ctatcgcctg gacgatgctt tcgctggtga tcgcctcgcc
     4621 catcagttcc gcgacatggg cgcggtcgcg caccaccacc acgcggtggc tgtaggcgac
     4681 aagttcgtcc agctccgagg aaatcaccag cagcgacatg ccgccggtgc aaagctcctc
     4741 gatcatgcgg atgatctcgg catgcgcgcc gacatcgatg ccgcgcgtcg gctcgtcgag
     4801 gatcaggaat tgcggattgg tcgccagcca gcgcgccaga agcacctttt gctggttgcc
     4861 gccggaaagc tggccgaccg gcttttccat gtccgaagtg cggatatcga gccggcggat
     4921 atagtcttgc gccagttcgc gctggcggcg cagcgatacc ggccgcatcc agccggtgcg
     4981 cgcctgaagc gccagcgcga tgttttcgcg cacgcccatg tcgccgatga tgccgctggt
     5041 cttgcggtct tccggcgaga agccgaagcc ggcggcaacc gcgtcgcgcg gcgaggccag
     5101 ccgcaacgcc ttgccgtcca tgctgacggt tccgctatcc atcggagccg cgccgaacat
     5161 cagctccacc gtttcggtgc ggcccgaacc gagcaggccg gcgacgccga taacctcgcc
     5221 gcgctggatg gcgaggtcga acgggctgat ggcgccgcgc cgcccggtgc cggaaaaacg
     5281 cagccgcggc tcggtggaat aatcgatgcc ggtgcggcca tggtggcggg tttcctcggc
     5341 cagttcgcgg cccagcatca aattgaccag ttgcaggcgg tcgagggcgg cgacatcgcg
     5401 cacgccgacc agcttgccgt tgcgcagcac ggtgatgcgg tccgaaatct cgaatacctg
     5461 gtcgaggaaa tgcgagatga agacgatgcc cagccccttt tcgcgcaggc cgcgcacgac
     5521 gtcgaacagc atgcgcacct catcgcggtc gaggctggcg gtcggttcgt cgaggatgag
     5581 cacgctgccc gacagcgaaa cggcgcgcgc aatggcgaca atctgctgga tggcgacgga
     5641 atagctgtcg agattgcgcg tcacgtcgag cgacaggccg tactgcgcca gcatctcgcg
     5701 cgcctggctg ttcatcgcct tgcgcgaaac cagtccgaag cggcagggct gccggcccag
     5761 catcaggttt tcagcgaccg tcaggttggg aagcaggttg acttcctgat agacggtgcc
     5821 gatgccaagc tgctgcgcgt gcagcgtatc gcgcggatcg atcgagcgtc cgtcgaggac
     5881 gatatcgccg ccatcgcggc ggatcgcgcc ggtgaggcac tttatcagcg tcgattttcc
     5941 tgcgccgttc tcgcccagca gggcgtggac ctcgccggcg cgcagatcga aatcaacgcc
     6001 gtcgagcgcc ttggtgccgg gaaaggattt cgtcagcgcg cgggcgctga gcagggtctg
     6061 gccttcgcct ggcgtcacga catgctccga tgcgactgga gttcgttggc ggcaatggcc
     6121 ggcagcgcaa gccgccggcc aataggctgc atcagtagcc gaggcccttc ttcagctcgt
     6181 acaccgcctt gggatcgtcc ttctgggtgt agagcttgga ttcggtctgg atgaatttcg
     6241 gcggaacggt gccgttgtcc ttataggcct tcagcgcgtc gaaggcggga ccggccatgt
     6301 tcggcgtcag ttcgaccgtc gcattggcct cgcccgccgc catcgcctgg aagatatccg
     6361 ggatcgaatc gacggagatg accttgatgt cggtgccggg cttgaggccc gcttccttga
     6421 tcgcctggat ggcgccgatg gccatgtcgt cattatgggc gtagagcgcg cagatgttct
     6481 tgccgccgtc ctccgccttg aggaagcttt ccatcacttc cttgcccttg gcgcgggtga
     6541 agtcgcccgt ctggctgcgg atgatcttga ggttggcgtg gccggcgatg gcctcctcga
     6601 agcccttctt gcggtcgatg gccggcgagg aaccggtggt gccctgcaat tcgacgatgt
     6661 tgcattcctt gctgccttca tcatcgacca gccactggcc ggcgaccttg ccctcatgca
     6721 cgagatcgga gccgatcgcg gtcaaataaa ggtcgggcga ggaatcgacc atgcggtcga
     6781 gcaggacgac cggaatgttg gcttccttgg cttccttcag cacggcgtcc cagccggtcg
     6841 cgacgaccgg agcgagcagg atggcgtcga cgccctgcgc aacgaaggaa cggatcgcct
     6901 tgatctggtt ttcctgcttc tgctgggcgt cggcgaattt gaggtcgatg ccgcgctttt
     6961 cggcttcctg cttggtcagc gtggtttccg ccgcgcgcca gccggattcc gaaccgattt
     7021 gcgcgaagcc gaccgtctcg gccagggccg cggacgtgaa cagggtaatc gacgcgagca
     7081 ggctcgcgcc aagcaatttc ctagcagtca tgaactcctc ccgaagtaag ggcaggtgtg
     7141 ccctgcccaa gaaaaaatca tacagtatga tatttgtaaa cagccgaccc cgaaattttc
     7201 ccggacgcgg gttttcttgc ccgaacgatt gcgatgcggt gcaccaccgc atgcagcacc
     7261 ccgcgtgcca tattgacttg cgcccgccgg tgtggaggct ggatttggcc caaccaatgg
     7321 aaattcctcg atgatctccg caaagaccgc tgccgacacg ttcaaccacc ctgtgcgcgc
     7381 gatggcgggc cgcgatccgc atgagacgca tcgcgtctcg acgccgctcg aactgctgtt
     7441 cgacctgact ttcgccaccg cgttcggatt tgccgcctcg cagctggccc acgcattggc
     7501 ggcgggccat tacacggccg cgctgatcgg cttcggcttc gccaccttcg ccatctgctg
     7561 ggcatggatc aatttttcat ggttcgcttc ggcctacgat accgacgact gggtgttccg
     7621 cgtcgttacc atggtgcaga tgaccggcgt gctggtgctg gcgatcgggc tgccgcgcat
     7681 gttcgcctcg gtcgagcacg gggaatatct cgacaattcg gtcatggtgt tgggctatgt
     7741 catcatgcgc ctggccatgg tgttccaatg gctgcgcgcg gcccggcagg accctgtgcg
     7801 ccaccgcgtc tgcctcactt atgcaatcgc catatcgatc gcgcagcttg gctggctgat
     7861 gcagatcttt tccagctttt ccgtcggcgc gagcataatc cttggcggca ttctcgccct
     7921 catcgaattg tccggccccg tcatcgccga gcgcaaggat ggcggcacgc cgtggcacgc
     7981 ccatcacatc gccgaacgct acagcctgtt tgccatcatc gcgcttggcg aaggcatcat
     8041 cggcacggtg gcggcgctgt cggcggcggt ggaggaacag ggctggagcc tcgacgccgc
     8101 cctggtctgc atcgccggcg tcgggctgac cttcggcatc tggtgggtct accacctgct
     8161 gccttccgcg caggttctgc acgccaaccg caccaggtcc ttcatatggg gatatggcca
     8221 gatggtcatc atcgcggcca tcgtcgccac cggcgcggga cttcatgtcg ccgcctattt
     8281 catcgagcac aaagccgcga tcggcccgct ggccacggtg ctgtgcgtcg ccgtgcctgt
     8341 cgcggttttc ctggtcggcg tctatgcact ctactatcat ctcgtgcgcc atttcgaccg
     8401 gctgcacaca tggctgctgc tcggcaccgc agcggtcgtg gtgctcgccg tccttgccgc
     8461 gcttgcaggc gtcgacatgg ccgtctgcct cgtcatcctg atgctggcgc cggccgtctc
     8521 cgtggtcggc tacgaaatgc aggggcacca ccacaccgag ggcgtgctgg ccgcaaaata
     8581 gagccgtcag cgcagccgcg agcggatctg ctcgtggacg acgcgcgagg ccggcgtcag
     8641 cgggcggtgg gcgggccgaa gcaacgaata gggcgcgacg acgaggtcgt cggccaccgg
     8701 cagcgtggcg atgacggaac ccagcccctc gccgctgccg aagaaggtgg cggcggcgcg
     8761 ggcaaccggt gaaatggcgt tggattgcgc gatcagcgcc agcgtcatca gcatggagga
     8821 ggtgctgatg acacgcgccg gcagcgcaat gccgcgttcc atcagatagg tttcgaccgt
     8881 ctggcgcatc agcccgccaa gcggctgcaa cacccagtcg aactcgacgc attcggcaag
     8941 gctcggactg tcgcgccgca tcagcggatg gccctgcctg acgatcaggc cgacaggctc
     9001 cgggccgatc ggatcggcga tgaaggcgtt gcggtcgaca tcgaccggca cccggccgat
     9061 gtaaaggtca agttcgtctg tcagcatcag cggcgccagc cggtcgctgg tatcgaccac
     9121 cacatggaca ttgatgcgcg gatgggtgac gcggatttgc cgcagcaccg gcagcacatg
     9181 ctccatggcc gcgcccgtca ccgccccgat ggcgacgaag ccccggcggc cggcatccat
     9241 gtcctcgatc tcgcgcgcgg cggcgtcgag gtcgcccagc atgcgccgcg ccagcgtcgc
     9301 gaaaaactcg cccgcctcgg tgaattcgac gccgcgcgca tgacgccggt agagcgcggt
     9361 tcccgatatc cgctcggctt cggccagcag gcgcgaggcg cccggctgcg aaatgccgag
     9421 gctggcggcg gccgcgctga tctgcccggt tttggccagc gtgccgagaa ggcgcagatg
     9481 gctgagcttg aggccccgcc gcagcagcct gtcggacccg gattcctgca tcgccttctc
     9541 cctctcgccg aaccatcata cctggaatga tatatctaaa cgaaataatt gtatttgacg
     9601 cacatatcac cacggaatag gccaatcgcg tgccggccat gagtttccgg ctaccaggga
     9661 ggaattcatg cgatcgcttc tacttgctct gggcacggct gccctcggcg cgctactcat
     9721 ttcgtcgtcc gcccaggcgc aggacaaggg cctcgtcggc atcgccatgc cgaccaagtc
     9781 gagcctgcgc tggatcaacg acggcgacag cctcaagaag tcgcttgagg aaaagggtta
     9841 caccgtcgac ctgcaatatg cagaagacga catccccaac cagctcgcgc agatcgagaa
     9901 catggtgacc aaggacgcca aggcgctcgt cgtcgcctcg atcgacggca ccacgctgtc
     9961 ggccgtgctc aagcaggccg ccgacaaggg cgtcaaggtc atcgcctatg accgcctgat
    10021 ccgcgacagc gccaatgtcg actactacac cacattcgac aacttcaagg tcggcgtgct
    10081 gcaagccgac tcgctcctga agggccttgg ctacccggag aagaagggtc cgttcaacat
    10141 cgagctgttc ggcggctcgc ccgacgacaa caacgccttc ttcttctatg acggtgccat
    10201 gtcggttctc cagccgctga tcgacaaggg cgatctcgtc gtgaagtccg gccagatggg
    10261 catggaaaag gtcggcacgc tgcgctggga cgcggcggtc gcacaggctc gtatggacaa
    10321 catcctgtcc gccaactact cggacggcag ccgcgtcgat gcggtgctgg cgccttatga
    10381 cggcctgtcg cgcggcatca tctcgtcgct gcgcggcgtc ggctacggca ccgccagcca
    10441 gccttggccg atcatttccg gccaggatgc cgagaccccc tcgatcaagg cgatcgtcgc
    10501 cggcgagcag tactcgaccg tcttcaagga cacccgcgaa ctcgccaagg tgaccgcgaa
    10561 catggtggat gccgttcttt ccggcggcaa gcccgagatc aacgacgaaa agacctacaa
    10621 taacggcgtc aaggtggttc cgtcctacct gctcgtgccg gtggcggtcg acaagtcgaa
    10681 ctacgagaag atcgtggtgg actccggcta catcaaggcc gaagacctga agtaacagac
    10741 gactgccggg aggccctttg cggggccttc cggaccagct ttgcgtttat acgccgcggt
    10801 tcagcgaccg cttgagcggg ctgagcctgc cgggaattcg ggagcaggac atgagcgacg
    10861 tcattctgga gatgcgcggc atcaccaaga cctttccggg cgtcaaggcg ctcgaaaacg
    10921 tcaatctcaa ggtaaagcgc ggcgatatcc acgccatctg cggcgaaaac ggtgccggca
    10981 aatcgacgct gatgaaggtt ctttccggcg tctacccgta tggcagctat agcggcgaca
    11041 tccagtacga aggcaaggag tgccgcttca acggcattaa cgacagcgaa aagctcggca
    11101 tcgtcatcat ccaccaggaa ctcgccctgg tgccgctgct ttccatcggc gagaacatct
    11161 ttctcggcaa cgagcaggcg aagaacggcg tcatcgactg gaaccgggtc cgccgccgct
    11221 cggctgagct tttgaagaaa gtcggcctgc gcgaaagccc cgacacgctg atcaccaata
    11281 tcggcaccgg caagcagcag ctggtggaaa tcgccaaggc gctgtcgaag gatgtcaagc
    11341 ttctgatcct cgacgagccg accgccagcc tttcggaaaa ggacagccag tcgctgctcg
    11401 accttctgct cgaattcaag gcgcagggca tgacttccat catgatctcg cacaagttga
    11461 acgaaatcaa gcgcatcgcc gaccagatca ccgtcatccg cgacggccag tcgatcgaga
    11521 ccatggacca gatcgacatc accgaggacc gcatcgtcac ctcgatggtc gggcgctcga
    11581 tggaaaaccg ctatccggcg cgcacgccca atatcggcga aaccgtcttc gaggtgaaga
    11641 actggtcggt ctaccatccg atccacccgg agcggcaggt catcaagggc atcaacctca
    11701 atgtccgcag gggcgaggtg gtcggcatcg ccgggctgat gggggccggg cgcaccgaat
    11761 tcgccatgag cgtgttcggc aagacctacg gcgccaacat cagcggcgaa gtgctgctgc
    11821 acggcaagcc agccgacacc tccagcgtca gcaaggcggt ggcagcgggg cttgcctacg
    11881 ccaccgagga ccgcaagacc tacggcctca acctgatcga ccacatcaag cacaatgtga
    11941 ccatcgccaa cctgccgggc atctccagtt cgggcgtcat cgacgacacg gcggaggtca
    12001 aggtcgccaa cgaatatcgc cgcaagacca acatccgctc gtccagcgtc taccagatcg
    12061 ccggcaacct ttcgggcggc aaccagcaga aggtcgtgct gtcgaaatgg ctgttcgcca
    12121 atccggacgt gctgatcctc gacgagccga cgcgcggcat tgatgtcggc gccaaatatg
    12181 agatctacac catcatcaac aagcttgccg aggaaggcaa aggcatcctc gtcatctcgt
    12241 cggaaatgcc ggaactgatc ggcatctgcg accgcatcta cgtgttgaac gaaggccgca
    12301 tcgtcggcga gatggcgggc gaagaggcga cgcaggaaaa gatcatgcgg gcgatcatgc
    12361 gcggcgaagc gcgggaagag cagatggaaa gggcagcgtc atgagcaccg aagtcaatgc
    12421 acctgcgacc agcgccgccg gcgcgtcggc ccatacggcg atcaaggaca atctgcgcga
    12481 atacggcctg gtcatcgcgc tgattgccat catgctgttc ttccagttca tgaccaatgg
    12541 cgtgctgttc cggccggtca acctgaccaa cgtcatcctg caaaattcct acatcgtcgt
    12601 gatggcgctc ggcatgcttc tggtgatcgt cgccggccat atcgacctgt cggtcggcgc
    12661 ggtcgccggc ttcatcggcg cggtggcggc catattgatg gtcgattacg gcatgaaccc
    12721 gcttctggcc ggtttcctgt gcatcctggc cggcgcagtg gtcggcggca tccagggcta
    12781 tttcatcgcc tatatgaaaa tcccgtcctt catcgtcacg ctggccggca tgctggtgtt
    12841 caagggcctg ctgctcaacg tattgagcgg ccgttctgtc gggccgttcc cggaagtgtt
    12901 ccaactgctc agcgccggct tcattcccga tgtgatcggg ccgatgacca tcatcgcgcc
    12961 gaccctcaac gcctcgggcg caccgacgcc gggaaccggc atcgtcctcc attcgacgac
    13021 gatgctgatc ggcgttttga tcgtcgccgc catgctctat ttcgcgctga aaagccgccg
    13081 caaccgcgaa agccatggct acgacgctga gcctttcgcg ttcttcgtcg ccaagaacct
    13141 ggtgatcgcg ggcatcgtcc tgttcctgat gttcaagttc gcctcctatc gcgggctgcc
    13201 caacgtgctg atggtgatgg gcgtgctcat cgcgctgttc gtcttcgtca ccacgcgcat
    13261 gacgtttggc cggcgcatct acgccatggg cggcaacatc aaggcggccg cgctgtcggg
    13321 catccagacc gaacggctga ccttctacat cttcgtcatc atgggcatgc tggcggccct
    13381 tgccggcctg atctattcgg cccgcctcaa catggcgacg ccaaaggccg gcgccgggct
    13441 ggagcttgac gtgatcgccg ccgtcttcgt cggcggcgca tcggcgcttg gcggcgtcgg
    13501 ccaggtgacg ggcgcggtga tcggcgcctt catcatgggc gtgatgaaca acggcatggg
    13561 cgtgatgggc atcaacatcg actggcagca ggtcatcaag ggcctggtgc tgctcggcgc
    13621 ggtcgcattc gacctctaca acaagaaaaa ggcctgattg ccatgctgct ctcccaaatc
    13681 ggtaatcccg atggctcgat caccgttgtg gcgcgcgaag gcaaggaggc ctatgcggta
    13741 aagggcgcgg acagcctcta tgccgtcgtg ctcgactgca tccgttcggg cagcgtcgat
    13801 ctcgccgccc atatcgcggc gctcgggctt ggctcggcgc tcgaccttga gcgcgcctat
    13861 gaggaagacc ggctcctccc gccgctgacc catcccgacc ccgcgcacct gcatctgacc
    13921 ggcaccggcc tcacccatct cggctcggcc gccacgcgcg acgccatgca caagaaggcc
    13981 ggcgcggaaa cggaagagaa gctgaccgat tccatgaaga tgtttcgctt ggggctcgag
    14041 ggcggcaagc ccgccaacgg caatgccggc gtgcagccgg aatggtttta caagggcaac
    14101 ggcacttcgc tggcggcgcc cggcggcacg ctcgcctcgc cggcctttgc cgaggattcg
    14161 ggcgaggaac ccgagatcgc cggcatctat gtcatcggcg aggacggcac gccgtttcgc
    14221 gtcggctttg cgctctccaa cgaattttcc gaccatgtga tggagcggcg caactacctc
    14281 tacctcgccc attccaagct gcggccggcc tcgtttggac cggaaatccg caccggcgcg
    14341 ctgcctgccg acatcagggg aacctcgcgc atccggcgcg gcaagaaggt gatcttcgaa
    14401 aagccattcc tgtcgggcga agcgaacatg tcgcattcga tcgccaacct ggaacaccac
    14461 catttcaaat atggcgtctt ccgccagccg ggcgacgtgc atgtgcatat gttcggaacg
    14521 gcgaccttgt ccttcgccga cggcatcagc accgaagccg gcgaccagtt cgagatcgag
    14581 gcggcgccct tcggcatgcc gctgcgcaac cgcctggccg tcgagaccgc cggcgcctgc
    14641 gcccgcgtga acgtgcgcgc gctctgagaa aggtaaagca ccatgccctt ccgcaaggcc
    14701 gaatggccta gaaaactgcg ctcgcacgaa tggtatggcg gttcgtccag ggacaatatc
    14761 taccaccgct cctggatgaa gaaccagggc ttgcccgccg acctgttcga tggccgcccg
    14821 gtgatcggca tctgcaacac ctggtcggaa ctcaccccct gcaatggcca tttgcgcgac
    14881 cttgccgaaa aggtgaagtt cggcatctat gaggcgggcg gcctgccggt cgagtttccg
    14941 gtgttctcca ccggcgaaag ctcgctgcgc cccaccgcca tgatgtaccg caacctcgcc
    15001 gccatggatg tggaggaagc gctgcgcgcc aatccgctcg acggcgtcgt gctgctggtc
    15061 ggctgcgaca agaccacgcc cgctttgctg atgggcgctg ccagcgtcga cattcccgcc
    15121 atcgccgttt ccggcgggcc gatgctgaac ggctggttcc gcggcgagcg cgtcggctcc
    15181 ggcaccgcac tttggcagat gtcggaggac atcaaggccg gcaagatgac gcgcgaggat
    15241 ttcctcgaag ccgagcaggc catgtcgcgc agccccggct cctgcaacac gatgggcacg
    15301 gcctcgacca tggcctcgat ggctgaggcg ctggggatgg cgctgtcggg caacgccgcc
    15361 attccagccg tcgacagccg ccgccgcgtc atggcgcatc tgaccggacg gcgtatcgtg
    15421 cagatggtca aggacgacct caagccttcc gacattctca cccgccaggc cttcgagaac
    15481 gccattcgcg tcaacggggc catcggcggc tcgaccaatg ccgtcatcca ccttctggcc
    15541 attgccgggc gcatcggcgt cgacctgacg ctggacgact gggaccggct tggccgcgac
    15601 gtgccgaccg tcgtcaattt gatgccatcc ggcaaatatc tgatggaaga gttcttctac
    15661 gccggcgggc tgccggttgt catcaagcgg cttggcgagg cgggcatgct gcacggcgat
    15721 gcgcttaccg tttccggcga ggcgatctgg gacgaggtca aggacgccgt caactggaac
    15781 gaggacgtca tcttgccggt ggagcgcgct ttgaccagcc atggcggcgt ggcggtgctg
    15841 aagggcaacc tcgcgccgaa gggcgcggtg ctgaagcctt ccgccgccag cccgcacctg
    15901 atgcagcatc gcggccgcgc cgtggtgttc gaggatatcg acgactacaa gcgcaagatc
    15961 gacgacgagg cgctcgacat cgacgagagc tgcatcatgg tgatgaagaa ttgcggcccg
    16021 cgcggctatc ccggcatggc cgaggtcggc aatatgggcc tgccgccgaa ggtgctgcgc
    16081 aagggcatca ccgacatggt gcgcatttcc gacgcccgta tgtccggcac agc
//
LOCUS       DASYCS010000094        12756 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2483462,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000094 DASYCS010000000
VERSION     DASYCS010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 12756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12756
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2483462"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="VGM46_03305"
     CDS             <1..334
                     /locus_tag="VGM46_03305"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012759270.1"
                     /note="Catalyzes the oxidation of dihydrolipoamide to
                     lipoamide; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEY4191648.1"
                     /translation="IASVGKTEEELKKAGVDYKVGKFPFSANGRARAMLHTDGFVKVL
                     ADKASDRVLGVHIVGFGAGEMIHGATVLMEFGGSSEDLGRVCHAHPTMSEAVKEAALA
                     AFFKPIHI"
     gene            complement(604..1020)
                     /locus_tag="VGM46_03310"
     CDS             complement(604..1020)
                     /locus_tag="VGM46_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191649.1"
                     /translation="MANPTNNPDPLDPLRNDPLNPIPPRTTADPVVNNRVAARSGGGA
                     GWLIALVVVVLAIIAYYVYGRTGPAAPPPAPAASESTPAPTTPAPATEPAPAATDTMK
                     PAAPAETAPAAPAPATPAPAEPAPAEPAPATPAPAN"
     gene            complement(1188..2255)
                     /locus_tag="VGM46_03315"
     CDS             complement(1188..2255)
                     /locus_tag="VGM46_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HEY4191650.1"
                     /translation="MKQALAIADRLSLPALKLLAALNVLFFLSFMIFLLFAAGKARAD
                     TPVCVGADLTVNLQKDDPAAYRKIVDEAAATPNGKGLLWKLEKPGQEPSYLFGTMHMT
                     DPRVTTLPASAQKALEAAGTVIIETTEVLDQKKMMATLAKEPDLMMFTDKTTLSSLLS
                     PTDAAAMAKALDERGIPALSVAKMKPWMLSAMVALPACELARKAGGAAVLDVKLAQDA
                     KAAGKGVEGLETAADQLRAMASLPMDFHMRGLVDTLKLGDKMNDVNETMIDLYQRGEI
                     GMIWPLFRVVLPDEADDPAGYAAFEEAMVTGRNKVMAEHAAPILAKGSAFMAVGALHL
                     PGAEGLVEEFRKAGYTVTAVN"
     gene            complement(2328..3269)
                     /locus_tag="VGM46_03320"
     CDS             complement(2328..3269)
                     /locus_tag="VGM46_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007007546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEY4191651.1"
                     /translation="MEEKFLLPAKSDLQAARESWLKMLARERRLSPETVEAYERDTRQ
                     FLQFLTGHRGGPPGIADIADLRPADLRGFLAARRAGGAGARTLGRGLAGIRSLLRFLE
                     RRGLVNAAGAAALRAPRQPKSLPKPLTASDAKHVVSVEGQLAEEPWIAARNAAVLTLL
                     YGSGLRISEALGLLAAELLSPRDTVLRITGKGGKTRLVPVLPVALKAVAEYRRLCPYH
                     LDPKGLLFRGARGGPLNPAIVQRDMAKLRSALNLPDTATPHALRHSFATHLLGRGGDL
                     RTIQELLGHASLSTTQVYTGVDTARLLEIYEAAHPRA"
     gene            3387..3995
                     /gene="rimM"
                     /locus_tag="VGM46_03325"
     CDS             3387..3995
                     /gene="rimM"
                     /locus_tag="VGM46_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582755.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEY4191652.1"
                     /translation="MPAPKNPIQMAVIGAPHGIKGELRIKAFTGDPLALGDYGPLYDK
                     DGRAFEIADIRPANTVVVVRFKNVKDRNAAEALAGTELFIDRSMLPDDGDEEEFYHAD
                     LVGLAVKDQTEGTIGQVDAVHNFGAGDILEVTLDGRRGVLIPFSQAAVPEVSISGGFV
                     RIDVAAAGLLDSEDDDAPAPEAEAGSGKPHRPQPSKKSGSGR"
     gene            3992..4693
                     /gene="trmD"
                     /locus_tag="VGM46_03330"
     CDS             3992..4693
                     /gene="trmD"
                     /locus_tag="VGM46_03330"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEY4191653.1"
                     /translation="MSFAASVLTLYPEMFPGALGLSLAGRALEAGTWSLEAIQIRDFA
                     GDRHRTVDDTPAGGGAGMVMRADVLAKAIDHASPEGDPRPRLLMSPRGKPLTQARVRE
                     LAAGPGAVILCGRFEGIDQRVIDARALEEVSVGDFILSGGEPAALVLLDAVVRLLPGV
                     MGNAESGNEESFENGLLEHPHYTRPQEFEGLPIPDVLTSGNHAKIAAWRRAQSERLTQ
                     ERRPDLLAAAQGLTK"
     gene            complement(4675..5427)
                     /locus_tag="VGM46_03335"
     CDS             complement(4675..5427)
                     /locus_tag="VGM46_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018430344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY4191654.1"
                     /translation="MSILDVIILFLAGYLSGVVNAMAGGGTFITFGAMSLLGTPPILA
                     NATSSVTQFPGYITSTLAYWSDIKLFWRGALVLCIISALGALAGSLILLGLDSASFKA
                     LVPWLLLAATALFAVGPWLKPKAKPAGEPKVGSLAGSIAQFFTAIYGGFFGAGMGVMM
                     LATLGLTESGDYHRINALKNMLAMVIASLAIVVFVAGGVVAWPEAIVMIPGGALGGYS
                     GVWIAKRVPQGVVRVFVIAVGVFLAVYYFVKP"
     gene            5750..6325
                     /gene="rplS"
                     /locus_tag="VGM46_03340"
     CDS             5750..6325
                     /gene="rplS"
                     /locus_tag="VGM46_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEY4191655.1"
                     /translation="MDILRQIEAEQAAKIEAKRTLPEFQPGDTVRVNVRVTEGTRTRV
                     QAYEGVVIARSGAGFQESFTVRKISYGEGVERVFPVYSPMVEGVEIVRRGKVRRAKLY
                     YLRDRRGKSARISENTGVRARKLNDAERDALHAEKARIEAEKIAAAQALAAEKAAQEA
                     AEKQAAAEAAKAADEAAKAAEAAAAAEPVAE"
     gene            6713..8125
                     /gene="leuC"
                     /locus_tag="VGM46_03345"
     CDS             6713..8125
                     /gene="leuC"
                     /locus_tag="VGM46_03345"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEY4191656.1"
                     /translation="MSAPRTLYDKIFDDHLVDQQDDGTCLLYIDRHLVHEVTSPQAFE
                     GLRMTGRQVRHPEKTLAVVDHNVPTSADRKLGINRNEESRIQIAALAQNAADFGVEYY
                     SETDRRQGIVHIIGPEQGFTLPGMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQ
                     TLIQRKAKNMLVRVDGKLPDGVTAKDIILAIIGEIGTAGGTGYVIEYAGEAIRALSME
                     GRMTVCNMSIEGGARAGLIAPDETTFAYVKDKPRAPKGENWDKAIEYWKTLHTDEGAH
                     YDKVIVLDAAKLPPIVTWGSSPEDVASVLGVVPNPDEIADENKRNSKKRALDYMGLEA
                     GTKITDIALDRVFIGSCTNGRIEDLRAVAKVVEGKTVASTVDAMIVPGSGLVKEQAEA
                     EGLDKIFIAAGFDWREPGCSMCLAMNDDRLKPHERCASTSNRNFEGRQGFKGRTHLVS
                     PAMAAAAAIAGHFVDIRDWK"
     gene            8514..9731
                     /locus_tag="VGM46_03350"
     CDS             8514..9731
                     /locus_tag="VGM46_03350"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006327094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY4191657.1"
                     /translation="MDTGLFIALLNPAIALVLASAFCLLWLNHRAGFHLAMLSVGYAT
                     SAIGFLLQYFSLPLIGFAMGRFISCLAFTIAALCLSTAILSRYGRHVPLAGLCLLAAC
                     GLAGFSWFLFVQPDLTWRIYAINFCFGGISLLVAAQLRPVRTHGPVQMALFVLSLLAA
                     CNFILRTLILVSIHGAFVSYDGFYASAYWTTTQLTHATLSLLIALCLFTAAALDMMGM
                     LKVESHTDPLSRLLNRRGFEERAAVLLDRCAKANLPVALVLADLDHFKIVNDVHGHAA
                     GDRVIVDFANKLRLAAGTRGVAGRLGGEEFAVLLPLADLGAARLFAEAVRTLFSAGPV
                     AGLPAPVRVTASFGVAARTGNEPLAPMLCRADEALYKAKRNGRDSVRLSYQRPEAPFP
                     AHPANAVPNVLTA"
     gene            9825..11039
                     /locus_tag="VGM46_03355"
     CDS             9825..11039
                     /locus_tag="VGM46_03355"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY4191658.1"
                     /translation="MTGALFILAINMAVAGLLAAAFMTIAVYDVRRVSARWMAAAYLL
                     GMFYFAIEFAIPAFSDAIPVVVVAFVVLLAGTLAFNIAIAHKYDVAPPWRWLLGLLIA
                     AAVLVSQVQDLPRQSLLRMGAYQIPYALMVLFAAGIVWRSPLRRQRLDYVLIALICAS
                     AAQFAAKPFVAQALGGWGANPQAYSRSIYASVSQAAGSVFLVAIALLLLVMIVRDVLA
                     DATAKSETDALSGLMNRRGFEDRALDALRSAVRHGVPLSLVIADLDNFKNVNDGFGHA
                     SGDRAIQAFASLLRGAVSGAHVAGRIGGEEFAILLPGSNLAAARLLAEGTRSAFSAVP
                     IAGLPADYRCTASFGVAQLGEGEEFGALMRRADHALYQAKRTGRDRVCVMPMPDASPA
                     RTAPVAQFNGKG"
     gene            complement(11023..11406)
                     /locus_tag="VGM46_03360"
     CDS             complement(11023..11406)
                     /locus_tag="VGM46_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191659.1"
                     /translation="MRSHTRHFGKSAVLALALAAFAAAPSAPAFAGSRHHDRVYADSF
                     GNLVIDSAAGYKRILVGEGRLAKKLADYTSAGQGAGEGAGQPHVVYADEYGHQDCYRP
                     PVFVKGRSYMYGLSDGEMPELSPCR"
     gene            11634..11999
                     /locus_tag="VGM46_03365"
     CDS             11634..11999
                     /locus_tag="VGM46_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191660.1"
                     /translation="MASRRNYRMASALMPLCLAAACASQPAAPSLAPTVSVPAPAPEI
                     SSQASTLTYNCADGGMMTIQNLGTSIRLLGPSGIDRELPASPPDQRSRYGKAHDAIII
                     DGRDALVVKGGQTPLACSQ"
     gene            12235..12651
                     /gene="sdhC"
                     /locus_tag="VGM46_03370"
     CDS             12235..12651
                     /gene="sdhC"
                     /locus_tag="VGM46_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEY4191661.1"
                     /translation="MSQSPATRAHAKPRPLSPHLTIYRPPITMTMSIVHRITGGALYV
                     GTLLVAFWLMAAAGPRPYFDFANGIFGSWIGQLILFGYTWALMHHMLGGIRHFVWDTG
                     AGLEKHTASKIAWATLIGSVLLTLAIWAVGYYVRGA"
     gene            12654..>12756
                     /locus_tag="VGM46_03375"
     CDS             12654..>12756
                     /locus_tag="VGM46_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006467972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEY4191662.1"
                     /translation="MSGGKNDMRTPLAKVRGLGSAREGTGHFWRQRLT"
BASE COUNT         2263 a         4187 c         4005 g         2301 t
ORIGIN      
        1 aatcgcctcg gtcggcaaga cggaagagga attgaagaag gcaggcgtcg actataaggt
       61 cggcaagttc cccttcagcg ccaacggccg cgcccgcgcc atgctgcata ccgacggttt
      121 cgtcaaagtc ctggccgaca aggccagcga ccgcgtgctt ggcgtccata tcgtcggctt
      181 cggcgccggc gagatgatcc acggagcgac ggtgctgatg gaattcggcg gttcgtccga
      241 agacctcggt cgcgtctgcc atgcccaccc gaccatgtcg gaagcggtga aggaagcagc
      301 gctcgccgca ttcttcaagc cgatccacat ctgacggtcg gccagcccgc aggctggcac
      361 tcgataaaat ccggaagtcg acaaacgaat ggcccgctaa ccagcgggcc gccagaccgc
      421 tggcaaactg ccaaaggtct cgaaatcacc caggaactct ggaaagtctc ggatttagac
      481 catcaacata ccaacgcccc tcgattttgg tcgccaccga aaccaagggg tttgtaatca
      541 gcctgacggc ccgccaataa gcgggccgtt ttcgtttcag gcggctgcct ggcggctgcc
      601 tggtcagttc gcgggggccg gcgttgccgg tgccggttca gccggggctg gctctgctgg
      661 cgcaggggtg gccggtgcgg gggccgcagg cgccgtttca gccggagcgg cgggcttcat
      721 cgtatctgtc gctgccggag ccggttctgt cgcgggcgcc ggtgtcgtgg gtgccggcgt
      781 gctttcgctt gccgcaggtg cgggcggcgg cgctgccggg cctgtgcgcc cgtagacata
      841 ataggcgata atggccagaa cgacgacgac cagcgcgatc agccagcctg cgccaccgcc
      901 ggaccgcgcg gcgacacggt tattgacgac cggatcggcc gtggtccgtg gcgggatggg
      961 attgagcgga tcattccgca gcggatcgag cggatccgga ttgttggttg ggttcgccat
     1021 tgcaatgtcc tccgtggatg aactgcgtca atcgaccttg ttcaacgcgc gatgggccat
     1081 tgggtttcac gagcaggtga attggctcga aaaagaaaga gtggcagggc gcggatggac
     1141 cctgtttcgg aagcctggaa cgtttcccga ctaatggaaa gcccgcctca attaaccgcc
     1201 gtcacagtgt agccggcctt gcggaattcc tcgacaagcc cctcagcgcc cggaagatgc
     1261 agcgcgccca ccgccatgaa agcgctgccc ttggccagga taggcgcggc gtgctcggcc
     1321 atcaccttgt tgcggccggt gaccatggct tcctcgaagg ccgcataacc ggccggatcg
     1381 tcggcctcat ccggcagcac gacgcggaac agcggccaga tcatgccgat ttcgccgcgc
     1441 tggtagagat cgatcatcgt ctcgttgacg tcgttcatct tgtcgcccag cttcagcgta
     1501 tcgaccaacc ccctcatgtg aaaatccatc ggcagcgagg ccatggcgcg aagctggtcg
     1561 gcggcggttt cgagaccctc cacgcctttg ccggccgcct tggcgtcctg cgccagcttc
     1621 acgtccagca cggccgcgcc acccgccttg cgggccagtt cgcaagccgg cagcgccacc
     1681 atcgccgaca gcatccaggg cttcatcttg gcaacactca gagccggaat gccgcgctcg
     1741 tccagcgcct tggccatcgc cgccgcatcg gtcggcgaca gcagcgacga cagggtcgtc
     1801 ttgtcggtaa acatcatcag gtccggctct ttggccagcg tcgccatcat cttcttctga
     1861 tcgagaactt cggtcgtctc gatgatgacc gtcccggctg cttctaaggc cttttgcgcg
     1921 gaggccggca gcgtggtgac gcgcggatcg gtcatatgca tggtgccgaa cagataggaa
     1981 ggttcctggc cgggcttttc cagcttccac agcagtccct tgccattcgg cgtcgcggcg
     2041 gcctcgtcga cgatcttccg gtaagcggcg gggtcgtctt tttgaagatt gaccgtcagg
     2101 tcggcgccga cgcaaaccgg cgtgtcggcg cgagccttgc ccgccgcaaa cagcaggaag
     2161 atcatgaacg acagaaagaa cagcacattg agcgccgcca gcagtttcag cgcgggcagc
     2221 gacaatcgat cggcgatggc gagggcttgc ttcatgccgg tatttctaag tgcaaaaagc
     2281 ggcgaaaccg ttaatcggca tggcgaaatt gtctccagcg catggcctca agcccttggg
     2341 tgcgccgcct cgtaaatttc cagcagccgg gccgtatcga cgccggtata gacctgcgtc
     2401 gtcgacaggc tggcatggcc cagaagttcc tggatggtgc gcaggtcgcc gccgcgtccc
     2461 aacagatgcg tggcgaacga atggcgcagc gcatgcggcg tcgcggtatc gggcaggttg
     2521 agcgccgagc gcagcttggc catgtcgcgc tggacgatcg ccggattgag cggaccaccc
     2581 cgcgcgccgc gaaacagcag gcccttcgga tcgagatggt aggggcacag ccgcctgtat
     2641 tcggcgaccg ctttcagcgc caccggcagc accggcacca gccgcgtctt gccgcccttg
     2701 ccggtaatgc gcagcaccgt atcgcgcggc gacaaaagct ccgccgccaa aaggccgagc
     2761 gcctcggaaa tgcgcaggcc ggagccgtag agcagcgtca gcacggcagc attgcgcgcc
     2821 gcgatccatg gctcttccgc caattggccc tcgactgaaa caacatgctt cgcgtcgctc
     2881 gccgtcagcg gtttgggcag cgatttcggc tgtcgcggcg cgcgcagggc ggccgcaccg
     2941 gcggcgttga cgagaccccg ccgctccagg aaccgcagca gcgagcggat gccggcaagg
     3001 ccgcgcccca gagttcgcgc cccagccccg ccggcacgcc gcgccgccag gaaaccgcgc
     3061 aggtcggctg gcctgaggtc ggcaatatcg gcgatgccgg gcgggccgcc gcgatggccg
     3121 gtcaggaatt gcaggaactg gcgcgtgtcg cgctcataag cctcgacggt ttcaggcgac
     3181 aggcggcgct cgcgcgccag catcttcagc cagctttcgc gcgccgcctg aaggtcggat
     3241 ttggccggga ggaggaattt ttcttccata agacctgctc gctcggggtc atcctgcctg
     3301 gtcgaggtta ggaaccggtg aagagcatgt gattgaaata gccgcgtccg aagtctagga
     3361 gacaggggaa atcaggattt tcgcctatgc ccgcgcccaa gaaccccatc cagatggccg
     3421 tgatcggcgc cccccacggc atcaagggcg aactgcgcat caaggccttt accggcgacc
     3481 cgctggcgct tggcgattat ggtccgctct atgacaagga tggccgcgcc ttcgagatcg
     3541 ccgacattcg cccggcgaat acggtcgtcg tcgtccgctt caagaacgtg aaggaccgca
     3601 acgccgccga agcgcttgcc ggaaccgaat tgttcatcga ccgctcgatg ctgcccgacg
     3661 atggcgacga ggaagaattc taccatgccg acctggtcgg gcttgcggta aaggaccaga
     3721 cggaagggac gatcggccag gtcgatgccg tgcacaattt cggcgccggc gacattctgg
     3781 aagtgacgct cgatggccgc aggggcgtgc tgatcccgtt cagccaggcc gccgtgccgg
     3841 aagtttccat ctccggcggg ttcgtccgga ttgatgtcgc ggcagctgga ttgctcgaca
     3901 gtgaagacga cgatgcgccc gcaccggagg ctgaagccgg ttcgggcaag ccgcaccgcc
     3961 cgcaaccatc gaaaaaatcc gggagcggcc ggtgagcttt gctgcctcgg tgctgacgct
     4021 ttatccggag atgtttcccg gcgcgcttgg cctttcgctg gccggccgcg cgctcgaagc
     4081 gggcacatgg tcgcttgaag cgatccagat ccgcgatttc gccggcgacc gccaccgcac
     4141 cgtcgacgat acgccagccg gcggcggcgc cggcatggtc atgcgcgccg acgttctggc
     4201 caaggccatc gaccacgcct cgcccgaagg cgaccctcga ccacgcctgc tgatgtcgcc
     4261 gcgcggaaaa ccgctgacgc aggcccgcgt gcgcgaactg gcggccgggc cgggcgcggt
     4321 gatcctgtgc gggcgcttcg aagggatcga ccagcgggtg atcgacgcgc gcgccttgga
     4381 ggaagtctcg gtcggcgatt tcatcctctc cggcggcgaa ccggcggcgc tggtgctgct
     4441 cgatgccgtc gtgcgcctgt tgcccggcgt catgggcaat gcggaatcag gcaacgaaga
     4501 aagcttcgag aacggcctgc tcgaacaccc gcactatacg cggccacagg agttcgaggg
     4561 cttgccgatc cccgatgtgc tgacttccgg caaccacgcg aagatcgccg catggcggcg
     4621 cgcccagtcg gaacggctga cgcaagagcg ccggcccgac ctgcttgccg ccgctcaggg
     4681 cttgacgaag taatacaccg ccaggaacac gccgaccgcg atgacgaaga cgcgcaccac
     4741 gccctgcggc acgcgcttgg cgatccacac gccggaatag ccgccgagcg cgccccctgg
     4801 gatcatgacg atcgcctccg gccaggcgac cacgccgccg gcgacgaaca ccacgatggc
     4861 aagcgaggcg atgaccatgg ccagcatgtt cttcagcgcg ttgatgcggt gatagtcgcc
     4921 gctttcggtg aggccgagcg tggcaagcat catcaccccc atgcccgcgc cgaagaaccc
     4981 gccatagatg gcggtgaaga actgcgcgat ggaaccggcc agcgagccta ctttcggctc
     5041 gcccgccggt ttggccttgg gcttcagcca ggggccgacc gcaaacagcg ccgtcgcggc
     5101 cagcagcagc cacggcacca gcgccttgaa cgatgcgctg tcgaggccaa gcaggatgag
     5161 cgatccggcc agcgcgccca gcgccgagat gatgcacaag acgagcgcgc cgcgccagaa
     5221 aagcttgatg tcgctccaat aggcgagcgt cgaggtgatg tagccgggaa actgcgtcac
     5281 tgaggaggtg gcgttggcga gaatgggcgg cgttccgagc agcgacatcg ccccgaaggt
     5341 gatgaacgtg ccgccgccgg ccatcgcatt gacgaccccc gacaaataac cggcgaggaa
     5401 aagaatgatc acgtcgagaa tggacattga aaggctgtca ccgggcggga ggggcaagga
     5461 ggatttatcc cccttacttt atctggcaat cttccttatg caggccgcgc gctaggctca
     5521 atagcgaaaa agtctcgggc caaggcgtga caaagccgca aaacatctgt atgtgcgcgc
     5581 tcaaaccggt ggaaacctgc cggatttgaa aaataacccg tcaacattgg cggtgcattc
     5641 gcccctgccg gaagttcttg aacggatggc atagtcgagc ggttcatgaa actgcaaggc
     5701 gagcgtcggg cgctctggct gtcggaagaa caagaagtgg atttttgaga tggatatcct
     5761 ccgtcagatc gaggccgaac aggccgccaa gatcgaagcc aagcgcaccc tgcccgaatt
     5821 ccagcccggc gataccgtcc gcgtcaatgt gcgcgtcacg gaaggcaccc gcacccgcgt
     5881 gcaggcctat gaaggcgtgg tcatcgcccg ttccggcgcc ggcttccagg aaagcttcac
     5941 cgtccgcaag atttcttacg gcgaaggcgt cgagcgcgtg ttccccgtct actcgccgat
     6001 ggtcgagggc gtcgagatcg tgcgccgcgg caaggttcgc cgcgccaagc tctattacct
     6061 gcgcgaccgt cgcggcaagt cggcccgtat ttccgaaaac accggcgttc gcgcccgcaa
     6121 gctgaacgat gccgagcgcg atgcgctgca tgccgagaag gcccgcatcg aggcggaaaa
     6181 gattgccgcc gcccaggcgc tcgctgctga aaaggccgcg caggaagccg ctgagaagca
     6241 ggctgccgcc gaagccgcaa aggccgccga tgaagctgca aaggctgccg aggcagctgc
     6301 cgccgccgag ccggttgctg aataattcca gccgtcggat aatccgaatt gaacaatgac
     6361 aaaggcggct tcggccgcct ttgtcatttg tgtcgcaggc ttcccgatga tcggcaacgg
     6421 atacggcgcg ccatctcgct tcggtcttga aaatttgacc cggcggcgcg tcaccgccgt
     6481 aattgccgtt tctccaaaac attgctatct agcaccgcat ggaaaagctt ttcagcgatc
     6541 cagcctataa gggcttcgtg ttgcaggacc acaagcgcct gccgtcgcgg ttcttcgcgc
     6601 gggtatgcgg ctccctgacc agccgactta tttaggctcc gcatcggcgc atgcggtccc
     6661 gtaagggcaa gcgccggctt ttttccatta ctccatcgac ggatttacgc acatgagcgc
     6721 accgcgcacc ctctacgaca agattttcga cgaccatctg gtcgaccagc aggatgacgg
     6781 cacctgcctg ctctacatcg accgccacct cgtccacgaa gtgaccagcc cgcaagcctt
     6841 cgaaggcctg cgcatgaccg gccgccaggt ccgccatccg gaaaagacgc tggccgtagt
     6901 cgatcacaac gtgccgacct cggccgaccg caagctcggc atcaaccgca acgaggaaag
     6961 ccgcatccag atcgcggcgc tggcgcagaa cgccgccgat ttcggcgtcg aatattattc
     7021 ggaaacggac cgccgccagg gcatcgtcca catcatcggc ccggagcagg gttttactct
     7081 gcccggcatg accatcgtgt gcggcgacag ccacacttcc acccatggcg cgttcggcgc
     7141 gctggcgcac ggcatcggca cctcggaagt cgagcatgtg ctggcgacgc agacgctcat
     7201 ccagcgcaag gccaagaaca tgctggtgcg cgtcgacggc aagctgcccg atggcgtcac
     7261 cgccaaggac atcatcctcg ccatcatcgg cgagatcggc accgccggcg gcaccggtta
     7321 tgtcatcgaa tatgccggcg aggccatccg cgcgctttcc atggaaggcc gcatgaccgt
     7381 ctgcaacatg agcattgagg gcggcgcccg cgccggcctg atcgcgcccg acgaaacgac
     7441 cttcgcctat gtcaaggaca agccgcgcgc ccccaagggt gagaactggg acaaggccat
     7501 cgaatactgg aagacgctgc acaccgacga aggcgcgcat tacgacaagg tgatcgtgct
     7561 ggacgccgcc aagctgccgc ccatcgtcac ctggggttcc tcgccggaag acgtcgcctc
     7621 ggtgctcggc gtggtgccga acccggacga gatcgcggac gagaacaagc gcaactcgaa
     7681 gaagcgcgcg ctcgactaca tgggcctcga agccggcacc aagatcaccg acatcgcgct
     7741 cgaccgggtt ttcatcggct cctgcaccaa tggccgcatc gaggatttgc gcgccgtggc
     7801 caaggtcgtc gagggcaaga cggtggcttc caccgtcgat gcgatgattg tgccgggttc
     7861 cgggctggtt aaggaacagg cggaagcgga agggctggac aagattttca tcgccgccgg
     7921 cttcgactgg cgcgagccgg gctgctcgat gtgcctggcc atgaacgacg accggctgaa
     7981 gccgcacgaa cgctgcgcct cgacctcgaa ccgtaatttc gagggccgtc agggtttcaa
     8041 gggccgcacg catctggttt cgccagcaat ggcggctgcg gcggcgattg ccggccattt
     8101 cgtcgacatt cgcgactgga aatagaccgg cgcgccatcg ttcagtcggc ttgccccaac
     8161 aggcttgccc tgcttaatcg caagaacccc gaagccatcc gctccggggt ttttttcgtt
     8221 cccgcacagg catcaatccg cgcggcgcat cgtttcgcac gaaggttccc gcatgtcggg
     8281 ccggtctccg gccaccactg cggccggccg tgggattgcc gggcaggcgg ctcgctttgc
     8341 cgattgccag gtcccgatgg tccgcatcgg tccccctcgt ctggatcgcc gcgccgcgcg
     8401 agaaacggga tgattttatc tggccaggaa gcgcgtcaga cccgtccggc ttaacgcgtt
     8461 gttcgtgctt gccagctagg cttgcaccga tcgaaacagc gaaatgagtt gtcttggata
     8521 ccggcctgtt cattgcgctg ctcaatccgg ctatcgcgct cgtgcttgcg tccgcgttct
     8581 gcctgctgtg gctgaaccac cgcgccggtt ttcatctggc gatgctctcg gtgggctacg
     8641 ccacctcggc catcggtttc ctgctccagt atttctcctt gccgttgatc ggtttcgcca
     8701 tgggcaggtt catctcctgc ctggcgttca ccattgcggc gctatgtctt tccacggcca
     8761 ttctctcccg ctacggcagg catgtcccgc ttgccggcct gtgccttttg gcggcctgcg
     8821 gcctggctgg cttctcgtgg ttcctgttcg ttcagccgga cctgacctgg cgcatctacg
     8881 ccatcaattt ctgcttcggc ggcataagcc tgctggtggc ggcgcagttg cgccccgttc
     8941 gtacccacgg gccggtgcag atggcgctgt tcgtgctgtc tttgctggcg gcctgcaatt
     9001 ttattctgcg caccttgatc ctcgtcagca tccacggcgc cttcgtcagc tatgacgggt
     9061 tttacgcctc ggcctactgg acgacgacgc agcttaccca cgcgacgctg tcgcttctga
     9121 tcgccctttg cctgttcacg gcggccgcgc tcgacatgat gggaatgctg aaggtcgaat
     9181 cgcataccga tcccctgtcc cggcttttga accgtcgcgg cttcgaggag cgcgcagcgg
     9241 ttttgctcga tcggtgcgcc aaggccaatc tgcccgtcgc gctggtgctg gccgatctcg
     9301 atcatttcaa gatcgtcaac gacgttcacg gacatgccgc cggtgatcgc gtcatcgtcg
     9361 acttcgccaa caagctgcgc ctggccgccg gcacgcgcgg cgtcgccggg cgtctgggcg
     9421 gcgaagaatt cgccgtgctt ttgccgttgg ccgatctcgg cgcggcgcgc ctgtttgcgg
     9481 aagccgtgcg caccctgttc tccgccggtc cggtcgccgg ccttcccgct ccggtcaggg
     9541 taaccgccag cttcggcgtc gccgcccgca cgggaaacga accgctcgcg cccatgcttt
     9601 gccgggccga cgaagcgctc tacaaggcca agcgcaacgg ccgcgacagc gtgcgccttt
     9661 cctatcagcg gcccgaagcg ccctttccgg cccaccccgc caacgcagtc ccgaacgtgc
     9721 tgacggccta gcgatcaaca ttcggcgagc ggtccggaat ccggcggcct gttaacgtct
     9781 tgttcgttcg tcgatggttt gcttgaagcc gagcggaaaa ttccatgacc ggggctttgt
     9841 tcattcttgc gatcaacatg gcggtcgccg gcttgctggc ggcagctttc atgacgatcg
     9901 ccgtctacga cgtgcgccgg gtttcggcgc gctggatggc tgccgcctat ctgcttggca
     9961 tgttctattt cgccatcgaa ttcgccattc ctgccttttc tgacgccatc cctgtagttg
    10021 ttgtggcttt cgtggtcctg ctggcgggaa cattggcgtt caacatcgcc atcgcccata
    10081 aatatgacgt ggctccgccg tggcgctggc tgctcggcct gctcatcgca gccgcagtcc
    10141 tcgtctcgca ggtgcaggat ttgccgcgcc agtccctgct gcggatgggg gcttaccaga
    10201 taccctatgc gctgatggtg ctttttgccg ccggcatcgt ctggcggtcg ccattgaggc
    10261 gtcagcggct ggactatgtg ctgatcgcgc tgatctgcgc cagcgcggcc cagttcgcgg
    10321 caaagccgtt cgtggcccag gcgctgggcg gctggggcgc caatccgcaa gcctactccc
    10381 gcagcatcta cgcttccgta tcgcaagccg ccggatcggt ttttctcgtc gccatcgccc
    10441 tgctcctgct ggtgatgatc gttcgcgacg tactggcgga cgccaccgcc aaatcggaaa
    10501 ccgacgcgct ttccggcctg atgaaccgcc ggggttttga agaccgcgcc ctcgacgcgc
    10561 tgcgttcggc cgtgcgccac ggcgttcccc tgtcgctggt gattgccgac ctcgacaatt
    10621 tcaagaacgt caatgacggt tttggccacg catccggcga ccgcgcgatc caggccttcg
    10681 cttccttgct gcgaggagcg gtcagcggcg cccatgtcgc cggccgcatc ggtggcgagg
    10741 aattcgcgat cctgctgccc ggctcgaacc tggcggcggc gaggctgctg gccgaaggca
    10801 cgcgtagcgc cttcagcgcg gtgccgatcg ccgggcttcc ggcggactat cgttgcacgg
    10861 caagctttgg cgttgcccaa ttaggcgaag gcgaggagtt cggcgcactg atgcggcgcg
    10921 ccgaccatgc cctctaccag gcgaagagaa ccggccgcga ccgcgtatgc gtcatgccga
    10981 tgcccgatgc gtcgccggcc aggacagcgc cggtggcgca attcaacggc aagggctgag
    11041 ttcgggcatt tccccgtccg acaagccgta catatagctg cggcccttca cgaataccgg
    11101 cggacggtag caatcctgat gcccatattc gtcggcatag acgacatgcg gctggccggc
    11161 gccctcaccg gccccctggc cggcgctcgt gtaatcggca agctttttgg ccagcctgcc
    11221 ttcgccgacg agaatgcgct tatagccggc ggcgctgtcg atgacgagat tgccgaacga
    11281 atcggcatag acgcggtcat ggtggcgcga gccggcaaag gccggggccg aaggggcggc
    11341 ggcaaaggcc gccagcgcca gagccagcac agccgatttg ccgaaatgcc gtgtgtgcga
    11401 acgcatgcgc gtcctccttg gttaaggtgg ggcggactcc tcgaatcaag aaattaacct
    11461 tttcttaacc tttgttaaga gacataacgg cccggctggc cgccttgcgc aaacatggtt
    11521 aacgcgagag gtttcaggcc cggccttagc gttgaccgac agccaccgac cgccggccca
    11581 tctccggcgt cgggcgggct gtccgatagc ggtttttgaa tggagatcag cccatggcgt
    11641 cacgccgcaa ctatcgcatg gcgagcgccc tcatgcccct gtgcctggcc gccgcctgcg
    11701 catcgcagcc cgccgcgccc tctcttgcgc caaccgtctc cgtgcctgcc ccagcgccgg
    11761 aaatatcctc gcaggcgagc acgctcacct ataattgcgc cgatggcggc atgatgacga
    11821 tccagaacct cggcacgtcg atccgcctgc ttggcccttc cggcatcgac cgcgaactgc
    11881 cggcatcgcc gcccgaccag cgaagccgct atggcaaggc gcacgacgca atcatcatcg
    11941 atgggcgtga cgcgctggtg gtcaagggcg gccagacgcc cctggcctgc tcgcaatgat
    12001 tcgtccgctt acgaccagtg gcgctttata ccgcagtgca gcgaccaggc cgcccactca
    12061 atcgattgaa gcgccgcagg tcacgaagac ttggccaaat tccgtccgct aaacggtttt
    12121 ccggctttga cgcggtgcaa aaagagtttt agaaaccggc aatccgaagt cgcatacgga
    12181 agcggccatg ccgctttccg cctggggccc caaggatatc gaactggggg agccatgagc
    12241 caatcaccag ccacccgcgc acacgccaaa ccgcgtccgc tttcgccgca tctgacgatt
    12301 tatcgtccgc ccatcaccat gacgatgtcg atcgtccacc gcatcaccgg cggggcgctc
    12361 tatgtcggca cgctgctggt cgccttctgg ctgatggcgg ctgccgggcc gaggccttat
    12421 ttcgacttcg ccaacggcat tttcggttca tggatcggcc agctgatcct gttcggctac
    12481 acctgggcgc tgatgcatca catgctcggc ggcatccgcc atttcgtctg ggataccggc
    12541 gccgggcttg aaaagcacac cgcctccaag atcgcctggg cgacgctgat cggctccgtg
    12601 cttctcacgc tcgccatctg ggccgtcggc tactacgtgc ggggggcctg accatgagtg
    12661 gcggcaagaa cgacatgcgc accccgctgg ccaaagtgcg cggcctcggc tcggcgcgcg
    12721 aaggcaccgg ccatttctgg cggcagcggc tgacgg
//
LOCUS       DASYCS010000095         9550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4369127,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000095 DASYCS010000000
VERSION     DASYCS010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 9550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9550
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4369127"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="VGM46_03380"
     CDS             complement(<1..923)
                     /locus_tag="VGM46_03380"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY4191663.1"
                     /translation="MKSHVKAVVIGGGVVGCSVLYHLAKAGWTDIMLIERSELTSGSS
                     WHAAGGFHTLNGDPNVAKLQAYTVQLYKELEELSGQSCSLHLTGGVMLADTPERMDFL
                     RLAHAKGRYLGMDTELITPSEAKAMFPLMDEKHFVGAMWDPVEGHLDPSGTTIAYSKA
                     AKKLGAEIVLRNPVKELTQEPDGTWNVITEQGTVHAEHVVNCGGLWAREIGRMVGVEL
                     PVLAMEHMYLLTEPMPEVEAFNKETGREMVGVLDFKGEIYTRQERNGILLGTYEKACK
                     PWSPVNTPWDFGQELLSPDLDRIADNLALGH"
     gene            complement(1005..2582)
                     /locus_tag="VGM46_03385"
     CDS             complement(1005..2582)
                     /locus_tag="VGM46_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007196399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEY4191664.1"
                     /translation="MNATPMPADTASAPDQPRRGGRAGKRASGSATFEQPPFRQLRNP
                     LTPTKLVSDDELESIHLASLRVLKEIGVDVLHDEARRIMKAHGADVREGSERVRFDAD
                     MILELISHCPAEFTIHARNPAHNVRFGGNNLVLSQMASAPNCSDIDNGRRPGNQRDFR
                     NFLKLAQMHNILNTTGGYPVEPTDIHPGVRHLECIRDLATLTDKVFHIYSLGKERNVD
                     GIEIARIARGISHEQLLEEPSVYTIINTNSPLKLDIPMMEGIIQMSGRGQVVIVTPFT
                     LSGAMAPVTIAGALVQQNAEALSGIAFAQMVRKGAPVGYGGFTSNVDMKSGAPAFGTP
                     EYMKAQLVGGQLARRYKIPYRTSNVCAANAVDAQAAYESVFSLWGAIQGGGNFILHGA
                     GWLEGGLRCSYEKTILDIDLLQMVAEFLTPLDLSEEALGVNAIRDVGPGGHFFGTQHT
                     QDRYKNAFYSPVISDWRNFESWAEAGSPTAVEKANRIWKERLAMYEEPYMDPAIREEL
                     NAFVKKRRAEGGAPTDF"
     gene            complement(2702..3766)
                     /locus_tag="VGM46_03390"
     CDS             complement(2702..3766)
                     /locus_tag="VGM46_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020010051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HEY4191665.1"
                     /translation="MAENAPASGIEVRSLYKVFGANARQAVELVRQGMSKAELNERHG
                     NVLGLKDINLEMPGGCIQVIMGLSGSGKSTLIRHINRLIDPTAGELLVGGVDVVKMNE
                     QELREFRRRNTAMVFQKFALLPHRTVLDNTVYGLQIRGVPRKRQVDEAMRWINRVGLA
                     GFENRYPNQLSGGMQQRVGLARALTNDAPILLMDEAFSALDPLIRTDMQGVLLDLQKE
                     IKKTVVFITHDLDEALRLGDRIAILRDGEVIQQGTGQEIVLNPADPYIEKFVREVNRA
                     RVLHASSVMTPFTGSSAPEGMAIAAGATLEDALREMVQSNSRIAHVLGEDGKPIGALT
                     MDALACAMVVPAPHGDGAGA"
     gene            complement(3759..4646)
                     /locus_tag="VGM46_03395"
     CDS             complement(3759..4646)
                     /locus_tag="VGM46_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007800569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline/glycine betaine ABC transporter
                     permease"
                     /protein_id="HEY4191666.1"
                     /translation="MDWLSSFPHLNDDLLRALKKSIDEGFRMFTRAYGDAIESFFDPL
                     QYFLIHSERFMTSTPWPIIMVIVAALAWLGSRSVKIVLGSLLTLFLIGLFDMWDDTMK
                     TISMIFVCTVLAIVLGIPIGILMARSNRVQSVVNPILDVMQTMPSFVYLIPVVMLLGI
                     GKVPGLIAVVIYAIPPMIRLTNLGIRLVDKDVLEAADAFGSTNWQKLKDVQMPLALPT
                     IMAGINQTIMMALAMVVIASMIGVQGLGQPVLKAISNQYFTLGVFNGLAIVGIAIIFD
                     RVSQAYGKRLQRHLEVVHG"
     gene            complement(4830..5831)
                     /locus_tag="VGM46_03400"
     CDS             complement(4830..5831)
                     /locus_tag="VGM46_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY4191667.1"
                     /translation="MKKLLASTALVAALFGFAATATAAECGDVTISNMNWQSAEVLAN
                     IDKIVLNAGYGCHAELVVGDTVPTLTSMTEKGEPDVAPEGWVDLLPDVVQKGIADKKL
                     VSTVEALSDGAVQGWWIPKYLADAHPAIKTIADALKHPELFPAPEDSSKGAVFNGPQG
                     WGGTLVTGQFFKAYDGEKAGFTLVDTGSAAGLDGSIAKAYERKEGWMGYYWAPTAILG
                     KYEMVKLDYGVPYDAAEWKRCNTVADCADPKPNEWPKDHVQTLVTTGFAERAGPAMDY
                     FSKRSWSNATVNKLLAWMTDNQATGEEGAKYFLKNSPDVWAAWVSPEVAEKVKKAIE"
     gene            6142..7305
                     /locus_tag="VGM46_03405"
     CDS             6142..7305
                     /locus_tag="VGM46_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015317447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4191668.1"
                     /translation="MAHARSSEHPTDWPAIAGVIATVSVFAIAQGLTYPLLSFILQRQ
                     GVSPAMIGLSAAMTPIGFIVSSPMVPMLARRFGSGRTALTCAALSALTLLLVSWTYSV
                     YPWFLLRFMIGLVTNPLYVLSETWVIALAPPSRRGRIMGIYATVISMGFAAGPLCLLA
                     VGTEGWAPFLVGIGAFVICGACLASVLHRLPKAETGGEHVSVWQFMPLAWLLMFTVVV
                     AAGFEQAVLALLPVYGAYYDIASSDMSALLTVMIAGNIAMQVPLGLLAERFSARPVRL
                     ACICLTVIGCMLLPVLIRTPWIWPCLFVWGSVSYGIYTMSIVELGERFTGKTMVAGNA
                     AFAMVWGVGGIVMPPLSGGAMALLGAPGLPLTLGLICLALALASAFTMLRDRR"
     gene            7302..8480
                     /locus_tag="VGM46_03410"
     CDS             7302..8480
                     /locus_tag="VGM46_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4191669.1"
                     /translation="MSAESLPDARLPVAALAGVIASVTVFGVAQGLSYPLFTFLMQKQ
                     GMSSGAIGLSAAMTPLGLMTSALFVPALVRLLGARGLAVGCATAAATLFLFVGLTQNW
                     LAWYPARFLIGLAVNPLYILGEVWLIALAPAARRGRILGVFNAITGAGYAAGPLTLAV
                     LGTDGWPPLMVGILGFLGCALILYLTANRLSSFDDGGRHGGVLHFWLIAPALMLAVVV
                     SAATQQSIYSLLPVFGAGYHMPETTIATLVGIMSIGNIVLQIPLGLLAERVGGRKMII
                     ACAAANLCGALLMPLLVTTPLQWPLLLIMGGVGYGVYTMALVELGNRFSGSALVAGNA
                     AFGLMWGLGGIIGPPGSGLFMQFAGPVGLPAVIATLSAILVLFATVRSLMARRGQGLQ
                     "
     gene            8521..8640
                     /locus_tag="VGM46_03415"
     CDS             8521..8640
                     /locus_tag="VGM46_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HEY4191670.1"
                     /translation="MFIAPPSTWPFASTPSPRTLRAELLLLCARIGYSPPAAS"
     gene            8694..>9550
                     /locus_tag="VGM46_03420"
     CDS             8694..>9550
                     /locus_tag="VGM46_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY4191671.1"
                     /translation="MTYQNYSLAQLQEIDAAHHLHPFTDHKELRAAGSRIITRAEGPY
                     IYDSEGVELLDGMAGLWCVNVGYGREELAKAAYDQMKELPYYNSFFKCSTPSPVLLAR
                     KLAEIAPKSVNQVFFGSSGSESNDTALRLVRHYWVLEGKPEKNRIISRHNAYHGSTVA
                     GTSLGGMEAMHKQLGGEVPNIVHVMMPYAYELALPGESDHDFGLRAAKAVEDAILEAG
                     AGNVAAFIGEPIMGAGGVKIPPMSYWPEVQRICRKYDVLLMLDEVITGYGRTGEWFAA
                     QSFGIEPDT"
BASE COUNT         1616 a         2865 c         3091 g         1978 t
ORIGIN      
        1 ttgtggccca aagccaggtt gtcggcgatc cggtcgaggt ccggcgacag caattcctgg
       61 ccgaaatccc acggcgtatt gaccggcgac cacggcttgc aggctttttc gtaggtgccg
      121 agcaggatgc cgttgcgctc ctgacgggtg taaatctcgc ccttgaagtc gagtacgccg
      181 accatttcgc ggccggtttc cttgttgaag gcctcgacct ccggcatcgg ctcggtgagc
      241 agatacatat gctccatggc cagtaccggc aattctacgc cgaccatgcg gccgatttcg
      301 cgcgcccaca ggccgccgca attgacgaca tgttcggcat gaaccgtgcc ctgttcggtg
      361 atgacgttcc aggtgccgtc gggctcctgc gtcagttcct tgaccgggtt gcgcagcacg
      421 atctcggcgc cgagcttctt ggccgccttg gaataggcga tggtggtgcc ggaggggtcg
      481 agatggcctt cgaccgggtc ccacatggcg ccgacgaagt gcttttcgtc catcaacggg
      541 aacatggcct tggcttcgga aggcgtgatc agttcggtat ccatgccgag atagcggccc
      601 ttggcgtggg ccagccgcag gaagtccatg cgctcgggcg tgtcggccaa catgacgccg
      661 ccggtcaggt gcagcgagca ggactggccg gaaagctcct ccagttcctt gtagagctgg
      721 acggtatagg cttgcagctt tgcgacgttc gggtcgccgt tcagcgtatg gaagccgccg
      781 gccgcatgcc aggacgaacc cgaggtgagt tcggagcgct cgatcagcat gatgtcggtc
      841 cagccggcct tggccagatg gtagaggacc gaacagccga cgacgccgcc gccgatgaca
      901 accgctttaa cgtgggattt catggtgttg aagctctcga tctagaaagt gaatcaagtt
      961 tggatcgacc ctctggtttg ggaccggagg gtttgaaatc tagttcaaaa atcggtgggc
     1021 gctccgcctt cggcgcggcg ctttttgacg aaggcgttca attcctcgcg aatggccggg
     1081 tccatgtagg gttcctcgta catggcaagc cgctccttcc agatgcggtt ggccttttcc
     1141 acggcggtcg gcgagccggc ttccgcccag ctttcgaaat tgcgccaatc ggaaatgacc
     1201 ggagaataga aagcgttctt gtagcggtcc tgcgtgtgct gggtgccgaa gaaatggccg
     1261 ccggggccga cgtcgcggat ggcgttgacg cccaatgcct cctctgacag gtcgagcggg
     1321 gtcaggaact ccgccaccat ctgaagcagg tctatatcga ggatggtctt ttcataggag
     1381 cagcgcaggc cgccttcgag ccagccggcg ccatgcagga tgaagttgcc gccgccctgg
     1441 atcgcgcccc acagcgaaaa aacgctttca taggccgcct gagcgtcgac cgcgttggcg
     1501 gcgcagacgt tcgaggtacg gtagggtatc ttgtagcggc gcgccaactg gccaccgaca
     1561 agctgcgcct tcatgtactc aggcgtgccg aatgccggcg cgcccgattt catatcgaca
     1621 ttggaggtga agccgccata gccgaccggc gcgccttttc tgaccatttg cgcaaaggcg
     1681 atgcccgaca gcgcctcggc gttctgctgc accagtgcgc cggcgatcgt caccggcgcc
     1741 atcgcgccgg acagcgtgaa tggcgtgacg atcaccacct ggccgcgtcc cgacatctgg
     1801 atgatgcctt ccatcatcgg aatatcgagc ttcagcggcg agttggtgtt gatgatggtg
     1861 taaaccgacg gctcttccag cagctgctca tggctgatgc cgcgcgcgat gcgggcgatc
     1921 tcgatgccat cgacattgcg ctccttgccc agcgaataga tgtgaaagac cttgtcggtc
     1981 agggtcgcca gatcgcggat acattcgaga tggcgcacgc cggggtgaat gtcggtgggt
     2041 tcgaccgggt agccaccggt ggtgttgagg atgttgtgca tctgcgccag cttgaggaaa
     2101 ttacggaaat cccgctggtt tccgggccgg cggccattat cgatatcgga gcagttcggc
     2161 gccgaggcca tttgcgacag gacgaggtta ttgccgccga agcgcacatt atgcgccgga
     2221 ttgcgggcat gaatggtgaa ttcggccggg caatgcgaaa tcagctcaag gatcatgtcg
     2281 gcgtcgaagc gcacgcgctc cgaaccttcg cgcacatccg cgccatgcgc cttcatgatg
     2341 cggcgggcct cgtcatgcag cacgtcgacg ccgatttcct tcagtacgcg cagcgaggcg
     2401 aggtggatcg attccagttc atcgtccgaa accagcttgg tcggcgtcag cgggttgcgc
     2461 aactgccgaa acggcggctg ctcgaacgtc gccgagccgg aggcgcgttt cccagcccgg
     2521 ccgccacggc gcggctggtc cggagccgaa gcggtgtcgg caggcatcgg cgtggcattc
     2581 atggcggcat cctcaggcaa tcggtatttt gaccgccata atggaccggc cgccacgccg
     2641 ccattgcgga ggcggcgacc actagggcaa tggaagcgac ataggccgat gcgccctcgc
     2701 tttatgctcc ggcgccgtct ccatgcgggg ccggcacgac catggcgcaa gccagcgcgt
     2761 ccatcgtcag cgctccaatt ggcttgccgt cttcgcccag aacatgggcg atgcgggagt
     2821 tggactggac catttcgcgc agggcgtctt caagggttgc cccggccgca atcgccatgc
     2881 cttcgggagc cgacgaaccg gtaaacggcg tcatcaccga cgaggcatga aggacgcggg
     2941 cgcggttcac ctcccggacg aacttttcga tgtaggggtc ggccgggttc agcacgattt
     3001 cctgccccgt gccctgctgg ataacctcgc cgtcgcgcaa gatggcgatg cgatcgccaa
     3061 gcctgagcgc ctcgtcgagg tcgtgggtga tgaagacgac ggtcttcttg atctcctttt
     3121 ggaggtcgag aagcacgccc tgcatgtcgg tgcggatcag cggatcgagc gcggaaaacg
     3181 cctcgtccat caaaaggatc ggcgcatcgt tggtcagcgc gcgcgccagg ccgacacgct
     3241 gctgcatgcc gcccgaaagc tggttgggat agcggttctc gaagccggca aggccgacgc
     3301 ggttgatcca gcgcatcgcc tcgtcgactt ggcgcttgcg cggcacgccg cggatttgca
     3361 gcccatagac cgtgttgtcc agcacggtgc gatgcggcag cagggcgaat ttctggaata
     3421 ccatggccgt gtttctgcgg cggaattcac gcagttcctg ctcgttcatc ttgacgacat
     3481 cgacgccgcc caccagcagt tcgccggcgg tagggtcgat caggcggttg atatggcgaa
     3541 tcagcgtcga tttgccggaa cccgacaggc ccatgatgac ctggatgcag ccgccgggca
     3601 tttcaagatt aatgtccttc aggccaagca cgttgccgtg gcgctcgttc agttcggcct
     3661 tgctcatgcc ttgccggacg agttcgactg cctgcctggc gttggcgccg aagaccttgt
     3721 agagcgagcg gacttcgatg ccggaagcgg gtgcgttttc agccatggac cacctccaga
     3781 tgcctttgca ggcgcttgcc atatgcctgg ctgacccggt cgaaaatgat ggcgatgccg
     3841 acgatggcga ggccgttgaa gacgcccagc gtgaaatact ggttggaaat cgccttcaga
     3901 accggctggc cgaggccttg aacgccgatc atggaggcga tgacgaccat ggccagcgcc
     3961 atcatgatgg tctggttgat gccggccatg atggtgggca gcgccagcgg catctgcacg
     4021 tccttcagtt tttgccagtt ggtcgagccg aaggcgtcgg cggcttccag cacatccttg
     4081 tcgaccagcc ggatgccgag attggtcagc cttatcatcg gcgggatggc gtagatgacg
     4141 acagcgatca ggcccggcac cttgccgatg ccaagcagca tgacgactgg gatgaggtag
     4201 acgaagctcg gcatggtctg catcacgtcg agaatggggt tgacgacgct ctgcacccgg
     4261 ttcgagcgcg ccatcaggat gccgatggga atgccgagca cgatggccag cacggtgcag
     4321 acgaagatca tggagatggt cttcatcgtg tcgtcccaca tgtcgaacag gccgatcagg
     4381 aacagcgtca gcagggagcc tagcacgatc ttgacgctgc ggctgccgag ccaggccagc
     4441 gcggcgacaa tgaccatgat gatcggccag ggcgtgctgg tcatgaagcg ttcggaatgg
     4501 atgagaaaat actgaagcgg atcgaagaaa gattcgatgg cgtcgccata agcgcgcgtg
     4561 aacatgcgaa aaccttcgtc gatgctcttc ttcaacgcgc gaagaagatc gtcgttcaga
     4621 tgcggaaagc tagataacca atccatcgcc ctgcctgtgt ttcccggtcc atgtgcatgc
     4681 gaatgtacaa gcacccaata gcacggggtt gccgggtaaa cccatgcaat tccagcggtt
     4741 ggggcgtgtc atttgaaacc cggccagctt tcgcgagcca ggcgatggag gcggcggcgc
     4801 gccatcgcct ccatcgttcg aaggccggtc tattcgattg ccttcttgac cttttcggca
     4861 acctccgggg agacccaggc tgcccagaca tcggggctgt tcttgaggaa gtatttcgcg
     4921 ccttcctcgc cggttgcctg gttgtcggtc atccaggcca gcagcttgtt gacggtggcg
     4981 ttcgaccatg agcgcttgga gaaataatcc atggccgggc cggcgcgttc ggcaaagccg
     5041 gtcgtcacca gcgtctggac gtggtctttc ggccattcgt tcggcttggg gtcggcgcaa
     5101 tcggcaaccg tgttgcaacg cttccactcg gccgcatcgt aaggcacgcc atagtccagc
     5161 ttgaccattt catatttgcc gaggatggcg gtgggcgccc agtaatagcc catccagcct
     5221 tccttgcgct cataggcctt ggcgatcgag ccatcgaggc cggcggccga accggtgtcc
     5281 accagcgtga agccggcctt ttcgccgtca taggccttga agaactggcc ggtcaccaaa
     5341 gtgccgcccc agccctgcgg cccgttgaag acggcgccct tggaagaatc ttccggggcg
     5401 gggaacagtt cgggatgctt cagcgcatcg gcaatggtct tgatggcggg gtgggcgtcg
     5461 gccagatatt tggggatcca ccagccttgc acggcgccat ccgagagcgc ctcgacggta
     5521 gacaccagct tcttgtcggc aatgcccttc tggacgacgt cgggcagcag atcgacccag
     5581 ccttcgggcg caacgtccgg ctcacccttt tcggtcatcg aggtcagcgt tggcaccgta
     5641 tcgccgacga ccagttccgc gtggcagcca tagccggcat tgagaacgat cttgtcgatg
     5701 ttggccagga cttcggccga ctgccagttc atgttggaaa tcgtcacgtc gccgcactcg
     5761 gcggcggtgg ctgtcgcggc aaagccgaac agagctgcaa ccaatgcggt tgaagcaaga
     5821 agcttcttca tcaggttcct cccggtttta gtccaatgtt tcgcttggca tcgaatgatc
     5881 gataccgtgg ccgaacgcct attgtcgatg aaagcgagac agctatcaac caatagccag
     5941 gactggtccg gatggtgctt cacagcgaca tgcgcttgtt tttttgcgac agcgccgcga
     6001 cttgccccgc ttatattgcc gtcgcttcaa aaagggcgtg gtgcaagcgg atttggcggg
     6061 gcggcacttc ctttcgcgtc ggcgagccga tagcacggct tctccgcgaa tcttgcggca
     6121 acctatggtg gcggcagatc tatggcacac gcccgttcca gcgaacatcc gacggattgg
     6181 ccagcgattg ccggcgtgat tgcgaccgtt tcggtgtttg cgatcgcgca ggggcttacc
     6241 tatccgctgt tgtccttcat cttgcagcgg cagggcgttt cgccggcgat gatcgggctt
     6301 tcggcggcca tgacgccgat cggcttcatc gtgtcgtcgc cgatggtgcc gatgctggcg
     6361 cgccgtttcg gttcggggag aaccgcgctc acctgtgcgg cgctttcggc gctgacgctg
     6421 ctgctggtca gctggaccta cagcgtctac ccatggttcc tgctgcgctt catgatcgga
     6481 ttggtgacta acccgcttta cgtgctgagc gagacgtggg tgatcgctct tgcgccgcca
     6541 tcgcgtcgcg gacgcatcat ggggatctat gccaccgtca tttctatggg ctttgcggcc
     6601 ggcccgctat gcctgcttgc ggtcggcacc gaagggtggg caccattcct ggtcgggatc
     6661 ggggcgttcg tcatttgcgg cgcctgcctg gcatccgtgc tgcaccggct gccgaaggcg
     6721 gaaacgggcg gcgagcatgt gtccgtgtgg cagttcatgc cgctggcgtg gcttttgatg
     6781 ttcacggtgg tggtggccgc cggcttcgag caggcagtgc tggcgctgct gccggtttat
     6841 ggcgcctact acgatatcgc gtcctccgac atgtcggcgc ttttgaccgt gatgatcgcc
     6901 ggcaatatcg ccatgcaggt gccgctcgga cttttggcag agcgattttc cgcccgtccg
     6961 gtgcggctgg catgcatctg cctgaccgtc attggctgca tgttgttgcc cgttttgatc
     7021 cgcactccct ggatctggcc atgcctgttc gtctggggat cggtttccta cggcatctat
     7081 acgatgtcga tcgtcgaact tggcgagcgc ttcaccggca agacgatggt tgccggcaat
     7141 gccgcctttg ccatggtctg gggcgtcggc ggcatcgtca tgccgccgct gagcggcggg
     7201 gcgatggcgc tgctcggcgc gccagggctt ccgctgacgc tcggcctgat ctgcctggcc
     7261 ctggcgctgg ccagcgcctt caccatgctg cgagaccgcc ggtgagcgcg gaaagccttc
     7321 ccgacgcgcg cttgcccgtt gccgcgctgg ccggcgtcat cgccagcgtc accgtgttcg
     7381 gcgtcgcgca gggtctgagc tatccgctgt tcaccttcct gatgcaaaag cagggcatgt
     7441 catcgggcgc gatcggcctt tcggcggcga tgacgccgtt ggggttgatg acttcggcgc
     7501 tgttcgtgcc ggcgctggtt cgccttctcg gcgcgagggg cctggctgtc ggctgtgcca
     7561 ccgccgcagc tacgctgttt ctgttcgtcg gcctgacgca gaactggttg gcctggtatc
     7621 cggcgcggtt cttgatcggg ctggccgtca atccgctcta tatattgggc gaggtctggc
     7681 tgatcgcgct ggcgccggcc gcgcggcgag ggcggatatt gggcgtgttc aacgccatta
     7741 ccggggctgg ctatgccgcc gggccgctga cgcttgccgt gctcggcacg gatgggtggc
     7801 cgcccctcat ggtgggcatt ttgggctttc tcggctgcgc gctgatcctt tacctcaccg
     7861 caaaccggct cagcagtttc gacgatggcg ggcgccatgg cggcgtcctg cacttctggc
     7921 tgatcgcccc ggccttgatg ctggcggtgg tggtgtctgc ggcgacgcag caaagcatct
     7981 actcgctgct gccggttttc ggcgccggtt atcacatgcc ggagacgacg atcgccacgc
     8041 tggtcggcat catgtccatc ggcaatatcg tgctgcaaat tccgcttggc ctgctggccg
     8101 agcgcgtcgg cgggcgaaag atgataatcg cctgcgccgc cgccaatctt tgcggggcgc
     8161 tgcttatgcc gttgctggtg acgacgccat tgcaatggcc gctgctgctg atcatgggcg
     8221 gcgtgggata tggcgtttac accatggcgc tggtcgagtt gggcaatcgc ttttcagggt
     8281 cggcgctggt ggccggaaat gcggccttcg gcctgatgtg gggactgggc ggcattatcg
     8341 ggccacccgg ttcaggcctt ttcatgcagt ttgccggccc tgtcggcctg ccggcggtta
     8401 ttgcgacgct gagcgccatc ctggtgctgt tcgccacggt ccgctcgctg atggcgcgcc
     8461 gtggccaggg gcttcaataa tcgtcctcga ttttgcatgg ggatgcggta aggttgcggc
     8521 atgttcatcg cgccgcccag cacatggccg ttcgcctcga cgccgtcgcc gcgcacgctt
     8581 cgcgccgaac tcttgctgct gtgcgcacgc atcggctatt cgccgcccgc cgcctcctga
     8641 ccaattcgcc tgacggcgct tgccgttttg agccaagagg aaaggctaaa cccatgacgt
     8701 atcagaatta ttcgctggcg cagcttcaag agatcgatgc cgcccatcat ctccatccct
     8761 tcaccgacca caaggaactg cgcgccgccg gttcacgcat catcacgcgc gccgaggggc
     8821 cgtatatcta cgattcggaa ggcgtcgagc ttctggacgg catggccggc ctatggtgcg
     8881 tgaatgtcgg ttatggccgc gaagagctgg ccaaggcggc ctatgaccag atgaaggaat
     8941 tgccgtacta caactccttc ttcaaatgct cgacgccgtc gccggtgctt ctggccagga
     9001 aactggctga aatcgcgccg aaatcggtaa accaggtgtt tttcggctcg tccggttcgg
     9061 aatccaacga tacggcgctg cggctggtgc gccattattg ggtgcttgag ggcaagccgg
     9121 aaaagaaccg catcatctcg cgccacaatg cctatcacgg ctcgacggtt gccggcacgt
     9181 cgctcggcgg catggaggcc atgcacaagc agcttggcgg cgaggtgccg aacattgtcc
     9241 atgtgatgat gccttacgcc tatgaactgg cgctgccggg cgagagcgac catgatttcg
     9301 gcctgcgggc ggccaaggcg gtcgaggacg ccattctcga agcgggcgcg ggcaatgtcg
     9361 ccgccttcat cggcgagccg atcatgggcg caggcggggt gaaaattccg ccgatgagct
     9421 attggccgga agtgcagcgc atctgccgca aatacgacgt gcttttgatg ctcgacgagg
     9481 tcatcaccgg ctatggccgc accggcgagt ggttcgcggc gcagtccttc ggcatcgagc
     9541 cggacaccat
//
LOCUS       DASYCS010000096         6501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2337581,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000096 DASYCS010000000
VERSION     DASYCS010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 6501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6501
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2337581"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..141
                     /locus_tag="VGM46_03425"
     CDS             <1..141
                     /locus_tag="VGM46_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY4191672.1"
                     /translation="EARNLDASGTGLGLAIARDIARSHGGDITLEDSPLGGLRAIIKV
                     PA"
     gene            complement(224..583)
                     /locus_tag="VGM46_03430"
     CDS             complement(224..583)
                     /locus_tag="VGM46_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HEY4191673.1"
                     /translation="MSAGSERKPEISFADFDKVDIRAGTIIEAEPFPEARKPAIKLKI
                     DFGPAIGVKKSSAQITKYYSPEALIGRQVFAVVNFPPRQIGPFMSEVLTLGFPDDEGA
                     VVLGAIERKVPDGGRLF"
     gene            complement(600..1100)
                     /locus_tag="VGM46_03435"
     CDS             complement(600..1100)
                     /locus_tag="VGM46_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013530009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HEY4191674.1"
                     /translation="MELLELEVSRDIHPVDVIEQVAHNNDWSFERAGDDEISISVTGS
                     WTDYHVSFSWMEDFEALHLACAFDIKVPEARTLEIMRLLSLINEQMLFGHFDLWEQEG
                     AIMFRQSLLLAGGVEPSSQQVEVLLSSALEACECYFQAFQFVVWSGTSAKDALAGVLF
                     ETHGNA"
     gene            complement(1422..1718)
                     /locus_tag="VGM46_03440"
     CDS             complement(1422..1718)
                     /locus_tag="VGM46_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEY4191675.1"
                     /translation="MSTGPNRILDEFAKLMTDAAGAAQGVRREVETAFRGQAERILNT
                     MDVVQREEFEAARDMAVKAREENAKLAARIEALEARLGASSDTPAAPAKPRAKK"
     gene            1862..2713
                     /gene="lgt"
                     /locus_tag="VGM46_03445"
     CDS             1862..2713
                     /gene="lgt"
                     /locus_tag="VGM46_03445"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013530007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEY4191676.1"
                     /translation="MSDYLLLPLAALPFPNIDPVIVTIGPLAIHWYGVGYIVGILFAW
                     WYARRLVTSPKLWPNGEFPMKPEALDDFIVWAAIGVVLGGRTGYVLFYDLPRYLANPL
                     DIFAVWQGGMSFHGGMLGTILAMVLFARSRGIRPWTLLDVVAAGVPVGLGLVRVANFI
                     NSELWGKLTDVPWAVEFPNGGPFPRHPSQIYEALLEGVVLFLVLRFLTHSRLKLKTPR
                     FVGGAFVCGYGLSRIFVEFFREPDAQLGYLFGGWLTMGMVLSLPMLLFGIWCMATAKP
                     AAAPQQA"
     gene            2710..3798
                     /locus_tag="VGM46_03450"
     CDS             2710..3798
                     /locus_tag="VGM46_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY4191677.1"
                     /translation="MTRLKRRIVELISALGPVPVSEYMALCLFDPQDGYYTTREPFGV
                     EGDFTTAPEISQMFGELVAVWLYEAWNALGRPMPVTFAEIGPGRGTLMKDMLRTLSRL
                     DAAFTANAAFAMVETSPRLAEIQKRTLAGSPATPGWHETVETLPDAPLIIVGNELFDA
                     VPIRQFVRTDTGWRERMVGLDDDENLAFFAGAASLDPPLLPADAGEAPLGAIVELAPA
                     RSAMMTAIAERIARRGGAGLFIDYGYLRPATGDTLQALRRHRHEDVLASPGEADLTAH
                     VDFAALAGIARAHGLGASAIDQGEFLLAMGLLERAGQLGRNAGQALRETLAGEVERLA
                     GPQAMGQLFKVLAIVSGGLTLAPFPLAD"
     gene            3898..4692
                     /gene="pgeF"
                     /locus_tag="VGM46_03455"
     CDS             3898..4692
                     /gene="pgeF"
                     /locus_tag="VGM46_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006338505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEY4191678.1"
                     /translation="MSNQTKPDPIRASLLEGMAARGIRHGYFTRTGGVSGGIYSGLNI
                     GAGSNDDQTLVQENRRRVAEWMGVAPSHLLTAYQIHSPDVVVAREPFSGDRPKADAIV
                     TDRPGIAIGASTADCGPVLFADAQARIIGAAHAGWKGAFTGVLENTITAMEGLGARRE
                     NIVAVLGPSIGPKNYEVGPEFVARFLDADAGNGRYFAPSATPGHSMFDLNLYTLDRLN
                     KAGVQADALGRCTYAEEDLFYSYRRTTHRGEPDYGRQVSAIVLENT"
     gene            4692..5843
                     /locus_tag="VGM46_03460"
     CDS             4692..5843
                     /locus_tag="VGM46_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004689854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEY4191679.1"
                     /translation="MALHFERSEFDARRDRLMIEMADKKLDAVLLFAQESMYWLTGYD
                     TFGFCFFQSLIVKADGSMVLLTRSADLRQARHTSIIENIVLWTDRDGANPAIDLRNLL
                     NDLDLLGKRIGVEYDTHGLTAFNGRRLDEQLQTFGQIADASGIVGRLRLFKSPAEVAK
                     AEKAANLSDDALDAALPLIKQGGDEAAILAAMQGTIFAGGGDYPANEFIIGSGPDALL
                     CRYKAGRRKLTKNDQLTLEWAGVFHHYHAPMMRTVLTGKVSKRHQELFDAARAALLAV
                     EKVMTPGNSFGDVFDAHARALEAHGLTKHRLNACGYSVGARFTPSWMDMPMFYQGNPE
                     PIAPNMTLFAHIIIADSETETAMTLGRTYLTTESQPKPLSRHDLDLIVQ"
     gene            5884..6474
                     /locus_tag="VGM46_03465"
     CDS             5884..6474
                     /locus_tag="VGM46_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191680.1"
                     /translation="MRRSHVTTVSLLMATVLCGCTRANDVLDPSAIKPTAGQAAPVAQ
                     GGAAPTVPAMPATQPASTATPAQSAAVMARIRLQIAPIVGPTVEAATPLTERLGLRAR
                     ERGLALTGSSGAQTPTHVLKGYFSAVSEGPDTTVIYVWDVYDPAGNRLHRINGQQKAP
                     SVKGGDGWTAVAPATMQAIADAAIEQLAAWLAANPG"
BASE COUNT         1176 a         2210 c         1923 g         1192 t
ORIGIN      
        1 gaggcccgaa atctcgacgc cagcggcacc ggccttgggc tggcaatcgc ccgcgacatt
       61 gcccgcagcc atggcggcga cataacgctg gaggacagcc cgctcggcgg actgcgcgca
      121 atcatcaagg ttccggcctg atcggtttaa gcgaaaaccg attccagtca gagactgcat
      181 gaaaacagcg ggttaaggca gctttctgaa tcagtttgaa aattcagaac agacggccgc
      241 catccggcac cttgcgctcg atggcgccaa gcaccactgc gccttcgtcg tcgggaaagc
      301 cgagcgtcag cacttccgac atgaatgggc cgatctggcg cggcggaaaa ttcaccaccg
      361 caaacacctg ccggccgatc agcgcttcgg gtgaataata tttggtgatc tgcgccgacg
      421 acttcttcac gccgatcgcc ggaccgaaat cgatcttcag cttgatcgcc ggcttgcggg
      481 cttcgggaaa cggctctgcc tcgataatgg tgccggcgcg gatatcgacc ttgtcgaaat
      541 ccgcaaagct gatttccggc tttctctcgc tgcccgcgct cattccggtt cagcctggct
      601 caggcgttgc catgcgtttc gaacagcacg ccggccagcg catctttggc cgaagtgccc
      661 gaccagacga cgaactggaa cgcctggaaa taacattcgc aagcctccag cgccgacgac
      721 agcagcacct cgacctgctg gctggacggc tcgacgccac cggcaagcag cagcgactgc
      781 cggaacatga tcgcgccttc ctgctcccac aggtcgaaat ggccaaacag catctgttcg
      841 ttgatgagcg acaacaggcg catgatttcc agcgtgcgcg cttctggcac cttgatgtcg
      901 aaggcgcagg cgagatgcag cgcctcgaaa tcctccatcc acgaaaacga gacgtgataa
      961 tcggtccagc tgccggtcac cgagatggaa atctcgtcgt cgcccgcacg ctcgaacgac
     1021 cagtcattat tatgcgcaac ctgctcgatc acatccaccg gatggatatc gcgcgagact
     1081 tcgagttcga ggagttccat gaatgcttcc tgtcgttccg acatggccgc cgcgcgtttc
     1141 gcggaatggt cacgcacacg aacaaaatct gtcttgaaca cgaacggtca ggattgctcg
     1201 acaccgatcc ccagatcgac cccaccaccc ggcgcatgac cccttccccg aatcatgcaa
     1261 caaattcagt ctattgatcg ggagtcgcct gaacagccta atttttcgca ccgcgtcatt
     1321 cgcatgtgga aagtcgctgt catgaagatt cacgcaatgc cggtaagcga ttcacggcaa
     1381 agcgcttttt caaggtctgg ctttgtggaa aagtttaacg attatttttt ggcacggggc
     1441 tttgcaggag ccgcaggtgt atctgaagag gccccaagcc tcgcctccaa cgcttcgata
     1501 cgggcggcga gtttggcatt ttcctcgcgc gccttcaccg ccatgtcacg ggccgcttcg
     1561 aattcctcgc gctgcaccac gtccatggtg ttgaggatac gctccgcctg gccgcgaaac
     1621 gccgtttcga cctcgcgccg caccccttgc gcggcaccgg cggcgtcggt catcagcttg
     1681 gcgaattcgt caagaatgcg gttgggtccg gtcgacatgg caaaacctcg cttggtagaa
     1741 ttgagttagt agggcatggc gaggaatgca aggtggtgct tgccttgacc ccgccaaaac
     1801 cctgccgcat gttccgcgcc aaatcgcccc ccttcaatgc ccctgcattc aggagatccc
     1861 gttgagcgac tatctgctcc tgccgcttgc ggccctgccc tttcccaaca ttgatcctgt
     1921 catcgtcacc atcggtccgc tggcgatcca ctggtacggc gtcggctaca tcgtcggcat
     1981 cctgtttgcc tggtggtatg caaggcgtct cgtcaccagc cccaagctgt ggccgaacgg
     2041 cgaattcccc atgaaaccgg aagcgctgga tgatttcatc gtctgggccg ccatcggcgt
     2101 cgtgttgggc gggcgaaccg gctacgtgct gttctacgac ctgccgcgct acctcgccaa
     2161 tccgctcgat atcttcgcgg tctggcaggg cggcatgtcg tttcacggcg gcatgctcgg
     2221 caccatcctc gccatggtgc tgtttgcgcg ctcgcgcggc ataaggccct ggactctgct
     2281 cgatgtcgtc gccgccggcg tgccggtcgg cctcggtctc gtccgcgtcg ccaatttcat
     2341 caattccgaa ctctggggca agctgaccga cgtgccgtgg gcggtcgaat tccccaatgg
     2401 cggtcccttc ccgcgccatc cgagccagat atacgaggcg ctgctggaag gcgtggtgct
     2461 gtttctggtg ctgcgcttcc tcacccattc gcggctgaaa ctgaagacgc cgcgcttcgt
     2521 cggcggcgct ttcgtctgcg gctacggcct gtcgcgcatt ttcgtcgagt tcttccgcga
     2581 acccgatgcg cagcttggct acctcttcgg cggctggctg accatgggca tggtcctgtc
     2641 gctgccgatg ctgctgttcg gcatctggtg catggccacc gccaaacccg cagcagcgcc
     2701 ccaacaggca tgacgcggct caaacggcgc atcgtcgagc tgatttcggc gctcggacct
     2761 gttcccgtca gcgaatatat ggcgctgtgc ctgttcgatc cgcaagacgg ctactacacg
     2821 acgcgcgaac cgttcggcgt cgagggcgac ttcaccacgg cgcccgaaat cagccagatg
     2881 ttcggcgaac tggtcgccgt ctggctttac gaggcgtgga acgcgctcgg ccggccaatg
     2941 cccgtcacct tcgccgaaat cggccccggc cggggcaccc tgatgaagga catgctgcgc
     3001 accctgtcgc gcctcgatgc cgctttcacc gccaatgccg ccttcgccat ggtagagacc
     3061 agcccaaggc tggccgaaat ccagaaacgg acgcttgccg gctcgcccgc aacgccaggc
     3121 tggcacgaaa ccgtcgaaac gctgcccgat gcgccgctga tcatcgtcgg aaacgaactg
     3181 ttcgacgcgg tccccatccg ccagttcgtg cgcaccgaca ccggctggcg cgagcgcatg
     3241 gtcggcctcg acgacgatga aaacctggcc ttcttcgccg gcgccgcctc gcttgacccg
     3301 ccgcttttgc ccgccgatgc aggcgaagcg ccactcggcg ccattgtcga gcttgcgccc
     3361 gcccgttcgg cgatgatgac ggcgattgcc gaacgcattg cccgccgcgg gggtgcaggc
     3421 ctgttcatcg actacggcta tttgaggcca gccaccggcg atacgctgca agcgctgcgc
     3481 aggcaccggc atgaggacgt gctggcgagt ccgggcgaag ccgacctcac cgcccatgtc
     3541 gatttcgcgg ccttggccgg cattgcgcgc gcgcacggcc ttggcgcaag cgccatcgac
     3601 cagggtgaat tcctgctcgc catgggcctg ctggaacgtg ccggccagct tggccgcaac
     3661 gccggtcaag ccttgcgcga aacgctcgcc ggcgaggtcg agcggcttgc aggcccgcag
     3721 gcgatgggcc agcttttcaa ggtcctggcg atcgtgtctg gcgggctgac cctggcgccg
     3781 ttcccgctgg ccgattgact ttcggcgcgg cgctgctcca ctgttgcgct ctctggacgg
     3841 gcaggcggcg accacacttt gcttgacgaa catcatggtg gagacaacaa agcggcaatg
     3901 tcgaatcaga ccaaaccgga tcccattcgc gcttccctcc tcgaagggat ggcggctcgc
     3961 ggcattcgcc acggctattt cacccgcacc ggcggcgtct ccggcggcat ctattccggc
     4021 cttaacatcg gcgcggggtc gaacgacgac cagacgctgg tgcaggaaaa ccgccgccgc
     4081 gtcgccgaat ggatgggcgt cgccccaagc catctcctga ccgcctatca gattcattcg
     4141 cccgatgtcg tcgtcgcgcg cgagccgttt tccggcgacc gccccaaggc cgacgccatc
     4201 gtcaccgacc ggccgggcat cgccatcggc gcatcgaccg ccgattgcgg ccccgtgctg
     4261 tttgccgacg cgcaggcgcg catcatcggc gcggcccatg ccggctggaa gggtgcgttc
     4321 accggcgtgc tggaaaacac cataaccgcc atggaaggcc ttggcgcgcg gcgcgaaaat
     4381 atcgtcgccg ttctcggccc ctccatcggc ccgaaaaact atgaggtcgg cccggaattc
     4441 gtcgcccgct tcctcgatgc cgatgccggc aatggccgct atttcgcacc ctcggcaacg
     4501 cccggccatt ccatgttcga cctcaacctc tacacgctcg accgcctgaa caaggccggc
     4561 gtgcaggccg acgcgctagg ccgctgcacc tatgccgagg aagacctgtt ctactcctac
     4621 cgccgcacca cccatcgcgg cgagccggat tacgggcggc aggtatccgc tatcgttttg
     4681 gagaatacgt aatggccctg catttcgaac gctcggaatt cgacgcgcgg cgcgaccggc
     4741 tgatgatcga gatggccgac aagaagctcg acgccgtgct cctgttcgcg caggaaagca
     4801 tgtactggct gaccggctac gacacgttcg gcttctgctt cttccagagc ctgatcgtca
     4861 aggctgacgg ctcgatggtg ctgctgaccc gctcggcaga tttgcgccag gcgcgccata
     4921 cctcgatcat cgaaaacatc gtgctgtgga ccgaccgcga cggcgccaac ccggccatcg
     4981 acctgcgcaa cctgctcaac gacctcgacc tactcggcaa gcgcatcggc gtcgaatacg
     5041 acacccacgg cttgaccgcc ttcaacggcc gtcgcctcga cgagcagttg cagaccttcg
     5101 gccagatcgc cgacgcttcc ggcatcgtcg gccggctgcg gctgttcaaa agcccggcgg
     5161 aagtcgccaa ggcggaaaag gcggccaacc tctccgacga cgcgctcgac gctgcgctgc
     5221 cgctcatcaa gcagggcggc gatgaagccg ctatcctggc cgccatgcag ggcacgatct
     5281 ttgccggcgg cggcgattat cccgccaacg aattcatcat cggctccggt ccggatgcgc
     5341 tgctttgccg gtacaaggcc ggccgccgca agctgaccaa gaacgaccag ctgacgctgg
     5401 aatgggcggg cgtgttccac cactaccacg cgccgatgat gcgcaccgtg ctcaccggca
     5461 aggtgtccaa gcgccatcag gagctgttcg acgccgcccg cgccgccttg ctggcagttg
     5521 aaaaggtaat gacgcccggc aacagtttcg gcgacgtttt cgacgctcat gcccgcgcct
     5581 tggaagccca tggcctgacc aagcaccggc tgaatgcctg cggctattcg gtcggcgcgc
     5641 gcttcacgcc ctcctggatg gacatgccga tgttctatca gggcaacccg gaaccgatcg
     5701 cgcccaatat gacgctgttt gcccacatca tcatcgccga ttcggaaacc gagaccgcga
     5761 tgacgcttgg ccgcacctat ctgaccacgg aatcgcagcc gaagccgctg tcgcgccatg
     5821 atctcgactt gatcgtgcag tgaatccgaa acggaagggc attcgccagg gagttttacg
     5881 ccgatgagac gatcgcatgt gacgaccgta tcattgctga tggcgacggt tctgtgtggc
     5941 tgcacccgcg ccaatgacgt gctggaccct tccgccatca agccgaccgc cgggcaggcc
     6001 gcgcctgtag cgcagggtgg cgctgcgcca accgtccccg ccatgccggc aacgcagccg
     6061 gcctcgacgg cgacgcccgc gcagagcgcg gcggtcatgg cccggatccg gttgcagatc
     6121 gcgccgatcg tcggcccgac ggtcgaggcc gcgaccccgc tcaccgaacg gctgggactg
     6181 cgcgcccgcg aacgcggact ggcgctgacc ggcagttccg gcgctcagac gccgacgcat
     6241 gtgctgaagg gttatttctc cgccgtttca gaaggcccgg acaccaccgt catctatgtc
     6301 tgggatgtat atgacccggc cggcaaccgc ctgcaccgca tcaacggcca gcagaaagcc
     6361 ccttccgtga agggcggcga cggctggacg gcggtagcac cggccaccat gcaggcgatt
     6421 gcagacgccg ccatcgagca gcttgccgca tggctcgcgg ccaaccccgg ctaaagtgtc
     6481 ccacgcagta atccaccgcc a
//
LOCUS       DASYCS010000097         9969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_5093745,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000097 DASYCS010000000
VERSION     DASYCS010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 9969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9969
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5093745"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1044)
                     /locus_tag="VGM46_03470"
     CDS             complement(<1..1044)
                     /locus_tag="VGM46_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019443342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HEY4191681.1"
                     /translation="MADAARKFSVAEQPLYREAPNNIEAEQALLGAILVNNDAFYRVS
                     DFLKSGHFYEPLHRKIFDVSAELIRMGKMANPVTIKTFLPADEKVGDMTVAHYLARLA
                     AEAVTVINAADYGRAIYDLATRRALITVGEDMVNIAYDAPVDMAPSEQIEDAERRLFE
                     LAETGRYDGGFESFTDAVKTAVDMANAAYMRDGHLSGIATGLRDLDSRMGGLQPSDLI
                     IIAGRPGMGKTSLATNMAFNIAEAYVPAQQADGSFKAANGGVVGFFSLEMSSEQLATR
                     IISEQTEIPSSKIRRGEISEMDFEKLVACSQTMQKIPLFIDQTGGISIAQLSARARRL
                     KRQRGLDVIVIDYI"
     gene            complement(1261..2232)
                     /locus_tag="VGM46_03475"
     CDS             complement(1261..2232)
                     /locus_tag="VGM46_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008594811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit Rsm22 family protein"
                     /protein_id="HEY4191682.1"
                     /translation="MELPAPLRQAVDRLLEDTPLAAIRQAAKTLSDRYRAELRDGRLH
                     MAQDLAVKAYLATRLPATYAAARASLDALREARPDFSPETLLDIGAGPGTVLWAAAQS
                     WPDLRQATLLEASEPARKIGQHLLAEGGLAIETAWLAGDATIELGGSAPADLVTLAYV
                     LDEIAPASLPKLIGKLWDLTADTLLIIEPGTPAGWRRILEARALLIATGAHILAPCPH
                     EIPCPLVASDWCHFSRRVARSRLHRLAKDAEVPWEDEKFIFVAASRRPAAHRAARVLA
                     APKSGSGKVALKLCRPDGEVEERLFTKRDGEVFRRARRADWGDTVED"
     gene            complement(2234..2557)
                     /locus_tag="VGM46_03480"
     CDS             complement(2234..2557)
                     /locus_tag="VGM46_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HEY4191683.1"
                     /translation="MTYLLYIAAALAEIAGCYSIWAWWRLEKPALWLVPGLACLILFG
                     WLLAQVQADAAGRAFAAYGGVYIAISLLWLWLAEGVRPDRWDIIGALLCIGGASTILF
                     APRGA"
     gene            complement(2757..4070)
                     /locus_tag="VGM46_03485"
     CDS             complement(2757..4070)
                     /locus_tag="VGM46_03485"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEY4191684.1"
                     /translation="MNVFLQRIVERIVHTGDLSITGPNGTTRRFGDGSGEKVHMVIHT
                     RHAERAITLHPTLAIPEAYMDEELDFIEGDVLSFLHLIYNNIGISWTDSTWGRLLNSA
                     RMTARPLQQWNTERRSKRNVERHYDLSGELYKLFLDEDMQYSCAYFERPDMTLEEAQL
                     AKKRHLAAKLSIKPGQNILDIGCGWGGLALYLAKNFESDVLGVTLSTEQHQVANDRIR
                     AQGLEDHVHIELRDYRDLSERFDRIVSVGMFEHVGVTQYRTFFDKCATLLKPDGVMVL
                     HTIGRTAPPTTTSAFIRKHIFPGGYIPALSEVTPHIERSGLAVTDVEILRLHYADTLK
                     HWRERFMANRDKVKEIYDERFCRMWEFYLCGSEASFRWQDLVVFQIQLTRKNDVLPMT
                     RDYIGKCEKALEMHEMGHQPGEAAATHGDRPKAKPVSKRKVSGTK"
     gene            complement(4218..4796)
                     /gene="rplI"
                     /locus_tag="VGM46_03490"
     CDS             complement(4218..4796)
                     /gene="rplI"
                     /locus_tag="VGM46_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEY4191685.1"
                     /translation="MDVILLERVARLGQMGDTVKVKDGFARNFLLPKGKALRANAANK
                     TKFEGQRAQLEARNLERKSEAQQIADTLDGKSFVAVRSAGETGQLYGSVSTRDIADLL
                     TAEGFSISRNQVELNHPIKTIGLTNVAVALHPEVEVTITLNIARTADEAERQAKGETL
                     TTAEAIYGEDINDNARPDNFFDPNAEFEGEEE"
     gene            complement(4997..5245)
                     /gene="rpsR"
                     /locus_tag="VGM46_03495"
     CDS             complement(4997..5245)
                     /gene="rpsR"
                     /locus_tag="VGM46_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEY4191686.1"
                     /translation="MVDINQIPTRRPFHRRRKTCPFSGANAPKIDYKDVRLLQRYISE
                     RGKIVPSRITAVSQKKQRELAKAIKRARFLGLLPYVVR"
     gene            complement(5245..5733)
                     /gene="rpsF"
                     /locus_tag="VGM46_03500"
     CDS             complement(5245..5733)
                     /gene="rpsF"
                     /locus_tag="VGM46_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEY4191687.1"
                     /translation="MALYEHVFLSRQDLSQQQVDELVERFKGVIAAGGGSVGRVENWG
                     LKSLTYRVNKNRKAYYTLMDITCPPAALNEMERQMGLSEDVLRFLTVKVENHEEGPSA
                     MMQKREERSERGGFGDRDRGPRSFGDRDRGDRPARSFGGDRDGGGDRGPRPQRSFEGS
                     AE"
     gene            complement(6020..7204)
                     /locus_tag="VGM46_03505"
     CDS             complement(6020..7204)
                     /locus_tag="VGM46_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4191688.1"
                     /translation="MTVEIQTEADERIHWLPMIAAISSISVVGIAIGLGMPLLSVILE
                     SRGHSATMIGLNTAVAGFASIAGAPLATPLAQRFGVAWTMLAMIATGALAFVGFHFAP
                     DFWMWFPLRIALHISLTVLFILSEFWISTSAPPRRRGLVLGVYATVLSLGFAAGPWLF
                     SRLGSTGFLPFGVTILLVVLAAIPVLMARKESPAIRAKGEETSGFLRYIWLVPTATAA
                     VLVFGAVETGGFALFPIYGNRIGYSETDAALLLTMIGLGNVLLQIPIGMISDRVKDRR
                     YLLMVCAFVGLLGAAFMPLFASNWHLMAALLFVWGGVVAAMYTVGLAHLGSKLSGHEL
                     ASANAAFVLCYGVGMVLGPQAIGIGMDAFGPNGFGWALAVFFGIYIILAVGRMAVGRS
                     QS"
     gene            complement(7224..7964)
                     /locus_tag="VGM46_03510"
     CDS             complement(7224..7964)
                     /locus_tag="VGM46_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY4191689.1"
                     /translation="MNELNRAQPQQAERSDVARAKGPLRPRDAATLILLERKGDEVFV
                     LMGRRSARHAFMPGKFVFPGGRTDPADSRIGVVAGLHPDEEATLRAGPGRMSAARVRA
                     IALSAIRETYEEAGLLIGRSGAFATTKRDWQGFVEHDVQPSLDTVRFIARAITPPGRV
                     RRFDTRFLSMWRSDVAKALPEGGPTNELEELVWLTLGQARNADIPGITRTILEELENR
                     LAVDPLLRPGGKVPFFRLVGKRYVRDLL"
     gene            complement(8037..8486)
                     /locus_tag="VGM46_03515"
     CDS             complement(8037..8486)
                     /locus_tag="VGM46_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HEY4191690.1"
                     /translation="MDVPMAQEQVFGGVHQSGRVARPLWQAMKFGFRGRCPHCGEGKL
                     FRSFVKPVDNCSVCGEDISHNRSDDLPAYLVIAIVGHIVVGGFMAVEVATVWPMWLHL
                     VVWLPLTVVLSLLLLQPIKGAVIGLQWANYMHGFGGEDDTIETHPEA"
     gene            complement(8488..>9969)
                     /locus_tag="VGM46_03520"
     CDS             complement(8488..>9969)
                     /locus_tag="VGM46_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015317635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HEY4191691.1"
                     /translation="WRAVPLVTIDPADAKDHDDAVFAEPDMDEKNPGGFIVTVAIADV
                     AAYVRHGTALDREALKRGNSVYFPDRVVPMLPERISNDLCSLREGENRPALAVRMTFS
                     IEGRKIRHSFHRVMMKSAAKLAYTQAQAAIDGAPDDKTGPILEAVLRPLWEAYAVLKR
                     GRDSRQPLELDLPERKIVLKEDGTVDRVIVPERLDAHKLIEEFMIQANVAAAETLEAK
                     KQPLLFRVHDAPSMAKQESLREFLQTIGLSLARGAQMRPSQFNGILERVRGEDNEGLV
                     NEVVLRSQSQAEYAPANIGHFGLNLRRYAHFTSPIRRYADLIVHRGLIGALGLGPDGL
                     TREEEARLEDVGVLISAAERRAMKAERDTVDRLIAAYLAERVDDRFDARISGVTKSGL
                     FVQLPQYGADGFIPVSSLGGDYYIYDEAARSLFGERTGKGYQLADRVEVRLLEVAPMA
                     GAMRFEMLTEPKPLPGSKRSFHKAKGQKQRGRASQPGSRGRRR"
BASE COUNT         1875 a         3101 c         3198 g         1795 t
ORIGIN      
        1 aatatagtcg atgacgatga cgtcgaggcc gcgctggcgc ttcagccgcc gcgcccgcgc
       61 cgaaagctgg gcgatggaga tgccgccggt ctgatcgatg aacagcggga ttttctgcat
      121 ggtctgcgaa caggcgacca gtttttcaaa gtccatctcg gaaatttcgc cacggcggat
      181 tttcgacgaa gggatttccg tctgctcgga aatgatgcgg gtggccagct gttccgacga
      241 catttcgagc gagaagaagc cgacgacgcc gccattggct gccttgaacg agccgtccgc
      301 ctgctgcgcc ggcacgtagg cctcggcgat attgaaggcc atgttggtgg cgagcgaggt
      361 cttgcccatg cctggacgcc cggcgatgat gataagatcg gagggttgaa gcccgcccat
      421 gcggctgtcg agatcgcgca ggccggtggc gatgccggaa agatgcccgt cgcgcatata
      481 ggcggcgttg gccatgtcga cggcggtctt caccgcgtcg gtgaagctct cgaagccgcc
      541 atcgtagcgg ccggtctcgg ccagttcgaa caggcggcgt tccgcgtcct cgatctgctc
      601 ggacggagcc atgtcgaccg gcgcgtcata ggcgatgttg accatgtctt caccaacggt
      661 gatcagcgcg cgccgcgtgg caaggtcata gatggcgcgg ccataatcgg cggcattgat
      721 gacggtgacc gcctcggcgg cgaggcgcgc cagataatgc gccaccgtca tgtcgccgac
      781 cttctcgtcg gcgggcagga acgtcttgat ggtcaccgga ttggccatct tgcccatgcg
      841 gatcaactcg gccgaaacgt cgaagatctt gcggtgcagc ggctcgtaga aatggccgga
      901 cttcaggaag tccgaaacac ggtagaaggc gtcgttgttg acgaggatag cgccgagcag
      961 cgcctgctcg gcctcgatat tgttcggggc ctcccggtag agcggttgtt ccgcaacact
     1021 gaatttccga gctgcgtctg ccatgtcatc cccgattctc gcctcgtatc tcgataccag
     1081 aacgagacaa gccggtgctg atttatctgc atcggtatcg atcgaaccga cattacttac
     1141 cgcgattcac agccttgcaa cgccgtggaa agattaaccg ttgactcgca ccgacattgc
     1201 gagtcgcagt cgcctttata gaacaaaaaa ggaacaaatt gcaagcgcct caggtaggct
     1261 tcaatcctcg actgtatcgc cccagtccgc cctgcgcgcg cgcctgaaca cctcgccgtc
     1321 gcgtttggtg aacagccgtt cctcaacttc gccatccggc cggcacaatt tcagcgccac
     1381 cttgccggag ccggatttgg gcgccgcaag cacccttgcc gcgcgatggg cggcgggccg
     1441 gcgcgaggcg gcgacgaaaa tgaacttctc gtcctcccat ggcacctcgg cgtccttggc
     1501 cagccgatgc aggcgcgagc gggcgacccg ccgggaaaaa tggcaccagt cggacgccac
     1561 aagcgggcag ggaatttcat gcggacaggg ggcgagaata tgtgctccag tggcgatcag
     1621 cagcgcgcgc gcttcgagga tgcgccgcca gccggcaggc gtgcctggct cgatgatgag
     1681 cagggtgtcg gcggtcaggt cccacagctt gccgatcagc ttgggtagcg aagccggcgc
     1741 gatttcatcg agcacatagg cgagcgtgac gagatcggct ggcgcggagc cgccaagttc
     1801 gatggtggcg tcgccggcaa gccacgccgt ttcaatggcc aggccaccct ctgcaagaag
     1861 gtgctgaccg attttgcgcg ccggctcgct tgcttccagc aatgttgcct gccgcagatc
     1921 gggccacgat tgggccgctg cccacaaaac cgtgccgggg ccggccccga tatcgagcaa
     1981 cgtttcgggt gaaaaatcgg gccgtgcttc gcgcaaggcg tcgaggcttg cgcgcgccgc
     2041 cgcataggtc gccggcagcc gcgttgccag ataagccttg acggcaaggt cctgcgccat
     2101 gtgtaaccgg ccgtcgcgca gttcggcgcg gtagcggtcg gacagcgttt tcgccgcctg
     2161 cctgattgcg gccaacggcg tgtcttcgag caggcgatcg accgcctgac gcaatggcgc
     2221 gggaagctcc acctcaggct ccccgaggtg caaacaggat ggtcgatgcg ccgccgatac
     2281 acagcagcgc gccgataata tcccagcggt cggggcggac gccttcagcc agccacagcc
     2341 agagcagcga aatggcgatg tagacgccgc cataagcggc aaaggcgcgc cctgcggcat
     2401 cggcctgcac ctgcgccaga agccagccga acaggatgag gcaggcgagg cccggcacaa
     2461 gccacagcgc cggtttttca agccgccacc aggcccagat ggaatagcag ccggcaatct
     2521 ctgccagcgc cgccgcgata tagagaagat aggtcatgaa ccggccccga aatgccggtg
     2581 tcttatcggc cgcgaatgcg acccttggca agcgcctgga ggcaggcggg tgtgacgagg
     2641 cctgaccggg gcattcaggc cgtgttcctc ccgctgctcg cggggaggtg ccgcatgcgg
     2701 cgaaacggac aggggaccgc cggacgccgc cctaaccaat ccaccgccgg aaccggctac
     2761 ttcgtgccgg aaaccttgcg cttgctgacg ggctttgcct ttggccggtc accgtgcgtg
     2821 gcggcggctt cgccgggctg gtggcccatt tcgtgcattt ccagcgcctt ttcgcacttg
     2881 ccgatatagt cgcgcgtcat cggcagcacg tcgtttttgc gggtcagctg gatctggaac
     2941 acgaccaaat cctgccagcg gaacgaggct tcggagccgc agaggtagaa ttcccacatc
     3001 cggcagaagc gttcgtcgta gatttccttg accttgtcgc ggttggccat gaagcgctcg
     3061 cgccagtgct tgagcgtgtc ggcgtagtgc aggcgcagga tttccacatc ggtgaccgca
     3121 aggccggagc gctcgatgtg cggcgtgact tccgacagcg ccggaatgta gccgcccgga
     3181 aagatgtgtt tgcggatgaa ggcgctggtg gtggtgggcg gcgcggtgcg cccgatcgtg
     3241 tgcagcacca tcaccccatc cggcttcagc aacgtcgcgc atttgtcgaa gaaggtgcgg
     3301 tattgcgtga cgccgacatg ctcgaacatg ccgaccgaaa cgatgcggtc gaagcgctcc
     3361 gagaggtcgc ggtagtcgcg caactcgata tggacatggt cttccaggcc ttgcgcgcgg
     3421 atacggtcgt tcgccacctg atgctgctcg gtcgacagcg tgacgccaag cacgtcggat
     3481 tcgaaattct tggcgagata gagcgccagg ccgccccagc cgcagcctat gtcgagaatg
     3541 ttctggccgg gcttgatgct gagctttgcc gcgaggtgcc gcttcttggc gagttgggcc
     3601 tcttccagcg tcatgtccgg gcgctcgaaa taggcgcagg aatattgcat gtcctcgtcg
     3661 aggaagagct tgtaaagctc gcccgagagg tcgtagtggc gctcgacatt gcgcttggag
     3721 cggcgctcgg tgttccattg ctgcaacggc cgcgccgtca tgcgggctga attgagcagc
     3781 cttccccatg tcgaatcggt ccaggaaatg ccgatattat tgtagatcag gtggaggaag
     3841 ctcaggacgt cgccctcgat gaaatcgagt tcctcgtcca tgtaggcttc gggaatggca
     3901 agcgtcgggt gaagcgtgat cgcgcgttcc gcatggcgcg tatggatgac catatgcacc
     3961 ttttcgccgg agccgtcgcc gaatcgacgg gtggtgccat tcggtcccgt tatgctgaga
     4021 tcaccggtgt ggacgatacg ctccacaatg cgttgcagaa atacattcat ggccaaacct
     4081 ataggcaaaa cacgttcatg gcgcgattgt gctaaaagcg aaggctcttg aaaagttcca
     4141 taatagaaaa aagcccggtc acaagggccg ggcttttatg ccagaaatgt ctctccggcg
     4201 ccaaaagacg atagagccta ttcctcttcg ccttcgaatt cggcgttcgg atcgaagaaa
     4261 ttatccggcc gggcattgtc gttgatgtct tcgccgtaga tggcctcggc ggtcgtcagc
     4321 gtctcgccct tggcctggcg ctcggcttcg tcggccgtgc gggcgatgtt gagcgtgatg
     4381 gtgacctcga cttccggatg cagcgctacc gccacattgg tcaggccgat ggtcttgatc
     4441 gggtggttga gttcaacctg gttgcggctg atcgagaagc cttccgccgt cagaaggtcg
     4501 gcgatgtcgc gggtggagac cgaaccgtag agctggccgg tttcgccggc cgagcggacg
     4561 gccacgaagc tcttgccgtc aagcgtgtcg gcaatctgct gggcttccga cttgcgctcg
     4621 aggttgcgcg cttcgagttg ggcgcgctgg ccttcgaatt tggtcttgtt ggcggcgttg
     4681 gcgcgcagcg ccttgccctt gggcaaaagg aagttgcggg cgaagccgtc cttcaccttg
     4741 acggtgtcgc ccatctggcc gaggcgggcc acgcgttcga gaagtatcac gtccatgatc
     4801 atgtaccttt tcatgtggcc cataatcggg ccgggaaacg aacaggtcag gacaaaaagg
     4861 ggcgtcgccg ccggtatcgc tgtcctgcct gtggcagtta gaggggcatc gccctcaact
     4921 cgggatttgt cgtcggccag ccatgcccgt cacggctgga gagaggcggc ggacgccaaa
     4981 cgcccgccgc aagaaatcag cgaaccacgt agggcagcag gccgaggaaa cgcgcgcgct
     5041 tgatcgcctt ggcgagttcg cgctgcttct tctggctgac ggcggtgatg cgcgacggca
     5101 cgatcttgcc gcgctcggaa atgtagcgct ggagaagacg cacgtccttg tagtcgatct
     5161 tcggtgcgtt ggcgccggag aaggggcagg tcttgcggcg gcgatggaac ggacgccggg
     5221 tcgggatctg gttgatgtcg accattattc tgcgctccct tcaaagctgc gctgcggacg
     5281 cgggccacgg tcgccgccgc catcgcggtc gccgccgaag ctgcgcgccg gacgatcgcc
     5341 acgatcgcgg tcaccgaacg aacgcgggcc gcggtcacgg tcgccgaagc caccgcgctc
     5401 ggagcgttct tcgcgcttct gcatcatggc cgacggacct tcttcgtggt tctcgacctt
     5461 gacggtcagg aagcgaagaa cgtcttcgga cagacccatc tggcgctcca tttcattgag
     5521 cgccgccggc gggcaggtga tgtccatcag cgtgtagtaa gccttccggt tcttgttgac
     5581 ccggtaggtg agggatttca gtccccagtt ctcgacccgg ccaaccgagc cgccgccagc
     5641 cgcgatgaca cccttgaaac gctcaacgag ttcatcgacc tgctgctgcg acagatcctg
     5701 ccgggaaagg aacacatgtt cgtaaagagc catattgttc tattgccttt cttcgtttct
     5761 cttccggtcc cggcggcgag agcctctgca acttctctga tccgccaggg cggagaagaa
     5821 aggagcacga cgagatggtc gagagcggag acacgggagg cggaagcgct tttgagcttc
     5881 acacggaggc tttcttgcga aaacctccgg ccctccgttc agcccccagc tgagaccggc
     5941 agattgccgg cgtctataca ggaaactggc cgcaacgcaa gtccagcgcc ggggaagggc
     6001 cggtattcgt cggccgatat caggattggc tgcgtccgac cgccatcctg cccaccgcca
     6061 gaatgatgta gatgccgaag aacaccgcca gcgcccagcc gaagccgttc gggccgaacg
     6121 catccatgcc gatgccgatc gcctgcggtc ccagcaccat gccgacgccg tagcacaaaa
     6181 cgaaggcggc attggccgat gccagttcat gccccgacag ctttgagccg agatgcgcaa
     6241 ggccgaccgt gtacatggcc gccaccacgc cgccccagac gaacagcagc gccgccatca
     6301 ggtgccagtt tgacgcaaac agcggcatga aagccgcgcc aagcaggccg acaaacgcgc
     6361 ataccatgag cagatagcgc cggtccttga cgcggtcgct gatcatgccg atgggaatct
     6421 gcaacagcac attgccaagc ccgatcatgg tcaaaagcag ggctgcgtcc gtctccgaat
     6481 agccgatgcg gttgccgtag atcggaaaca gcgcaaagcc gccggtctcg accgcgccga
     6541 acaccagaac ggcggcagtc gccgtcggca ccagccagat atagcgcagg aagccgctgg
     6601 tttcctcgcc tttggcgcgg atagcggggc tttccttgcg cgccatcagc accgggatgg
     6661 cggccagcac caccagcagg atggtgacgc cgaagggcag gaagccggtg ctgccaaggc
     6721 gcgaaaacag ccatggcccg gcggcgaagc cgagcgacag caccgtcgcg tagacgccca
     6781 gcaccagccc gcgccgtcgc ggcggcgccg aggtgctgat ccagaactcc gaaagaatga
     6841 acagcacggt gagcgagata tgcagcgcta tgcgcagcgg aaaccacatc cagaaatcgg
     6901 gagcgaaatg gaagccgaca aaggcaagcg ccccggtcgc gatcatggcc agcatcgtcc
     6961 acgcaacgcc gaatcgctgc gccagcggcg tcgccagcgg ggcgccggca atggaggcga
     7021 agccggcgac ggcggtgttc agcccgatca tcgttgccga atgcccgcgc gattcgagaa
     7081 tgacgctgag cagcggcatg ccgaggccga tggcgatgcc caccacgctg atggacgaaa
     7141 tcgccgctat catcggcagc cagtggatgc gctcgtcagc ttccgtttgg atttcgaccg
     7201 tcatgaaatg aatgcctaaa ctgttacagg agatcgcgga cgtaacgctt gccgacaagc
     7261 cggaagaagg gaacttttcc gccaggccgc agcagcgggt ccacggcgag gcggttttcc
     7321 agctcttcca atatcgtgcg ggtaatgccc ggtatatcgg cattgcgggc ctggcccagc
     7381 gtcagccaga ccaattcctc aagctcgttc gtcggtccgc cctcaggcag ggccttggcg
     7441 acgtcgctgc gccacatgct gaggaaccgc gtatcgaacc ggcgcacccg gccgggcggg
     7501 gtgatggcgc gggcaatgaa gcgcacggtg tcgagcgaag gctgcacatc atgttcgacg
     7561 aaaccttgcc agtcccgctt ggtggtggcg aaagcgccgc tgcgcccaat gagcagcccc
     7621 gcttcctcat aggtctcgcg gatggccgac agggcgatgg cgcgcacgcg ggcggcgctc
     7681 atgcggccgg gaccggcgcg aagcgtggct tcctcgtcgg gatgcaggcc ggccaccacg
     7741 ccgatgcggc tgtccgccgg atcggtacgg ccgcctggaa acacgaattt gcccggcatg
     7801 aaggcatgac gcgcgctgcg ccggcccatc agcacgaaaa cctcgtcgcc tttgcgctca
     7861 agcaggatca gcgttgccgc gtcgcgtggc cgcaacggcc cttttgcacg agccacatcg
     7921 ctgcgttcgg cttgctgagg ttgcgcccgg ttcaactcat tcatgggcac gacctagcgg
     7981 aaacttactg ccgatgaaag ggacgcacgc aaccgcaaga ttgcgccagc gctgctttat
     8041 gcttccgggt gggtctcgat ggtatcgtcc tcgccgccga agccgtgcat gtaattggcc
     8101 cattgcaagc cgatgaccgc gcctttgatc ggttgcagca aaagcagcga cagcacgacc
     8161 gtcaacggca gccataccac gaggtgcagc cacatcggcc atacggtcgc gacttccaca
     8221 gccatgaagc cgccgacgac gatgtggccg acgatggcga tgaccagata ggccggcagg
     8281 tcgtcggagc ggttatggga aatgtcttcc ccgcacaccg aacaattatc gaccggcttg
     8341 acgaaggagc gaaacagctt gccttcgccg caatgcgggc agcggccgcg aaagccgaat
     8401 ttcatcgcct gccacagcgg cctggcaacg cggcccgact ggtgcactcc gccgaaaacc
     8461 tgctcttgcg ccattggaac atccatttca acgtctcctg ccgcgcgaac ccggctgcga
     8521 tgcgcggccg cgctgctttt ggcccttcgc cttgtggaac gaacgcttcg atccaggcaa
     8581 gggcttcggc tcggtcaaca tctcgaagcg cattgcgccg gccatcggcg ctacttccag
     8641 cagccgaacc tcgacgcggt cggcaagctg atagcctttt cctgtgcgct ccccgaatag
     8701 agaccgggct gcttcgtcgt atatatagta gtcgccgccg agagatgaaa ccgggataaa
     8761 gccgtctgcg ccatactgcg gcaattggac aaacagcccc gatttggtga cgcccgagat
     8821 gcgggcgtcg aagcggtcgt ccacgcgttc ggccagatag gcggcgatca gccggtccac
     8881 cgtatcacgc tcggccttca tggcgcgccg ttccgccgcc gagatcagca cgccgacatc
     8941 ttccagccgt gcttcttcct cgcgcgtcag cccgtcgggt ccaagcccca gcgcgccgat
     9001 cagcccgcga tgcacgatca ggtcggcata gcggcggatc ggcgaggtga aatgggcata
     9061 gcgccgcaga ttgaggccga aatggccgat attggcgggc gcgtattcgg cctggctttg
     9121 cgaacgcagc accacctcgt tgacaagccc ttcattgtct tcgccgcgca cccgctcaag
     9181 aatgccgttg aactggctcg gccgcatctg cgcgccgcgc gccagcgaca ggccgatcgt
     9241 ctgcaagaat tcgcgcagcg attcctgctt ggccatcgac ggcgcgtcat ggacgcggaa
     9301 aagcagcggt tgcttcttcg cctccagcgt ctctgcggcc gccacattgg cctggatcat
     9361 gaattcttcg atgagtttgt gcgcatcgag ccgctccggc acgatgacac ggtcgacggt
     9421 gccgtcttcc ttcagcacga tcttgcgttc cggcaggtcg agttccagcg gctggcggct
     9481 gtcgcggccg cgtttcagca cggcgtaggc ctcccataga ggcctcagca ccgcctcaag
     9541 gatcgggccg gtcttgtcgt cgggcgcgcc gtcgatggcc gcttgagctt gcgtataagc
     9601 gagcttcgcg gccgatttca tcatgacgcg gtgaaacgaa tgccggatct tgcggccctc
     9661 gatcgaaaac gtcatgcgca cagccagcgc cgggcggttc tcgccttcgc gcagcgagca
     9721 gaggtcgttg gaaatgcgct ccggcagcat cggcacgacc cggtcgggga aatagaccga
     9781 attgccgcgc ttcagcgcct cgcggtcaag cgccgtgccg tggcgcacat aagcggcgac
     9841 gtcggcgatg gcgacggtga cgatgaagcc gccggggttc ttctcgtcca tgtcgggttc
     9901 ggcgaacacg gcatcgtcat ggtccttggc gtcggccgga tcgatggtga cgagcggcac
     9961 cgcccgcca
//
LOCUS       DASYCS010000098         3869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3644240,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000098 DASYCS010000000
VERSION     DASYCS010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3869
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3644240"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /gene="purH"
                     /locus_tag="VGM46_03525"
     CDS             <1..241
                     /gene="purH"
                     /locus_tag="VGM46_03525"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008936310.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEY4191692.1"
                     /translation="ARKALDAAEAAGLPEPLTKGSVVASDAFFPFADGLLSAIEAGAT
                     AVIQPGGSMRDDEVIAAADAHGIAMVFTGTRHFRH"
     gene            complement(250..1626)
                     /locus_tag="VGM46_03530"
     CDS             complement(250..1626)
                     /locus_tag="VGM46_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4191693.1"
                     /translation="MAETEVQRASGRGIWGWMLFDWAAQPFFTVVTTFIFGPYFVSRM
                     ASDPALGQAAWGYGIAAAGLVIAVLSPVLGSISDQTGPRKPWIGFFAIVQIVSLSLLW
                     FAAPGSNLYVAVLFFSLASVAAEFSTVFNDSMMPRLVPKSEIGRISNIAWGLGYLGGM
                     IALIFVVGFLAGSPETGKTIIGATPLFGLDPKLGEDARATGPLSALWYLVFILPMFFY
                     TPDSKRGIPIGPAVRAGLAELKATIAEVRERGGIFRFLIARMLYQDGVNALLALGGAF
                     AAAMFGWSITEIGIYGIILNIVAIFGCLVAARLDSSLGSKKMVMVSLVLLTIATLGIV
                     STGPGYTLLGAWPLPGVDSGGLFGTPAEKAYIAFGLLVGLAFGPVQASSRSYMARSVT
                     AEESGRYFGIYALAGRATSFAAPFTVATITALSGSPRLGMAAIVLFLGAGMWLLAGTP
                     YPADRPKG"
     gene            complement(1793..>3869)
                     /locus_tag="VGM46_03535"
     CDS             complement(1793..>3869)
                     /locus_tag="VGM46_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018430179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
                     /protein_id="HEY4191694.1"
                     /translation="TATFSDTANAISQAHDFWLDDAFASGGSAGYDHKKMGITAKGAW
                     EAVKRHFREMNRDIQATPFTMAGVGDMSGDVFGNGALRSQETRLIAAFDHRDIFIDPD
                     PDMAASFAERQRLFDLPRSSWQDYDKSKLSAGGVIVSRSQKSITLPQAAAAAIGLGKT
                     VASPTEIMRAILAAPVDLLWFGGIGTYLRGTTETNQEVGDRANDAIRLTALDVRAKVI
                     GEGANLGVTQRARIEFGLKGGLCNSDAIDNSGGVNCSDVEVNIKIALASAMRKGSLTR
                     PARNKLLADMTDEVGHLVLANNYEQTLALSLARKRGLTDIVHQGRFMEALETRGLLNR
                     TVETLPSPAGLAEREARGEPLTRAELGVLLAYAKIVLFSDIVASDVPDDPHFDRDLMG
                     YFPERMAKKYTAEIHGHRLRREIIARVVANDLVNRGGPSFVNRLQESTGRSAADVVRT
                     FAAVRDGFGLPALYREIDALDNRIDGQIQLDLYQTVSRLIYTTCGWYLKNETASVPLG
                     KWIADLVEVRKALEPKLLSLLPEFSRQRIEERKHGLFKGGAPEKLAERLALTDIAELI
                     PDIALTAQAANAGIVAAAKAFFTVSEAFRIARIEDAVHSISPSDYYDQLALSRAIDTI
                     GVARRGMAVAALTAYAGAPDPVAAWLEAGGENIARVRARLQALTEGGDLTVSRLSVAS
                     GLMSDLTIR"
BASE COUNT          684 a         1200 c         1362 g          623 t
ORIGIN      
        1 cgcgcgcaag gcgctggacg ccgccgaggc ggcaggcctg ccggagcctt tgaccaaggg
       61 ttcggtggtt gcctccgacg cctttttccc gtttgccgat ggcctgctgt cggccatcga
      121 ggccggcgcc accgccgtta tccagccggg cggatcgatg cgcgacgacg aggtgatcgc
      181 cgccgccgat gcgcacggca tcgccatggt gtttacgggg acgcgtcact tccggcacta
      241 ggcataggac taccctttcg gcctgtccgc cgggtagggt gtgccggcca ggagccacat
      301 gccggcgccg aggaacagca cgattgccgc catgccgagg cggggcgagc cgctcaaggc
      361 ggttatggtg gcgacggtga acggcgcggc gaaactcgtc gcccgcccgg ccagcgcata
      421 gatgccgaaa tagcggcccg attcctccgc cgtgacgctg cgcgccatgt aggaacgcga
      481 ggacgcctgc accgggccga aggcaaggcc gaccagaagg ccgaaagcga tataggcttt
      541 ttccgccggc gtgccgaaca ggccgcctga atcgacgccg ggcagcggcc atgcgccgag
      601 cagcgtatag cccggtccgg tcgaaacgat gccgagcgtg gcgatagtca gcaggaccag
      661 cgagaccatc accatcttct tcgagccgag cgatgaatcc agccgtgcgg cgaccaggca
      721 gccgaagatg gcgacgatgt tgaggatgat gccgtagatg ccgatctcgg tgatcgacca
      781 gccgaacatc gccgccgcaa aggcgccgcc cagcgccagc agcgcgttga cgccgtcctg
      841 atagagcatg cgggcgatga ggaagcggaa gatgccgccg cgttcgcgta cttccgcgat
      901 ggtcgccttc agttccgcca gccccgcgcg caccgccggg ccgatgggga tgccccgttt
      961 cgaatccggc gtgtagaaaa acatcggcag gatgaagacc agataccaca gcgccgaaag
     1021 cgggccagtg gcgcgcgcgt cctcgccgag cttcgggtcg aggccgaaca gcggcgttgc
     1081 gccgatgatg gtcttgccgg tttccggcga accggcgagg aagccgacga cgaagatcag
     1141 cgcgatcatg ccgccgagat agccaaggcc ccaggcaata ttggaaatcc tgccgatttc
     1201 gcttttcggc actaggcggg gcatcatgga atcgttgaac acggtcgaaa actcggccgc
     1261 caccgaagcc agcgagaaga acagcacggc gacatagagg ttggagccgg gcgcggcgaa
     1321 ccacagcagc gagaggctga cgatctgcac gatggcgaaa aagccgatcc atggctttcg
     1381 cggtccggtc tggtcggaga tggagccgag taccggcgac agcacggcaa tgacgaggcc
     1441 ggcggcggcg atgccatatc cccatgccgc ctggccgagt gccgggtcgc tggccatgcg
     1501 cgagacgaaa tacgggccga agatgaaggt ggtgacgact gtgaagaagg gctgcgcggc
     1561 ccaatcgaaa agcatccagc cccagatgcc ccggcccgat gcgcgctgca cttctgtttc
     1621 ggccatggat gctcccgtac ccggtgcgca tgtgtgcaca ttgtccggcg gcatgtaaat
     1681 gggccggtgg cgatgtaaac ggcccaactg gacgcaaatg ggctggagtg gccgcaagcg
     1741 gtctgcgaga cggaaatagc ggcgccgacc ctgaggccga tgccgccatc gatcaccgga
     1801 tggtcaggtc gctcatcagt cccgacgcca ccgaaagacg cgagacggtc aggtcgccgc
     1861 cctcggtcag ggcttgaagg cgcgcgcgca cccgcgcgat gttctcgccg cccgcttcga
     1921 gccacgccgc caccgggtcc ggcgcgccgg cataggcggt gagcgccgcc accgccatgc
     1981 cgcgccgggc gacgccgatg gtgtcgatgg cgcgcgacag cgccagttga tcgtaatagt
     2041 cggagggcga aatggaatgc accgcgtcct cgatgcgggc gatgcggaag gcttcgctga
     2101 cggtgaagaa cgccttggcg gcggcaacga tcccggcatt ggccgcctgc gccgtcaggg
     2161 cgatgtcggg gatcagttcg gcgatgtcgg tcaaagccag ccgttcggcc agcttttccg
     2221 gcgcgccgcc cttgaacagg ccatgcttgc gttcctcgat gcgctggcgc gagaattccg
     2281 gcagcagcga aagcagcttc ggctccagtg ccttgcggac ctcgaccaga tcggcgatcc
     2341 acttgcccag cggcacagag gcggtctcgt tcttcaaata ccagccgcag gtcgtgtaga
     2401 tgagccggct gaccgtctgg tagagatcga gctggatttg cccgtcgatc ctgttgtcga
     2461 gcgcgtcgat ttcgcggtag agcgccggca ggccaaagcc gtcgcggacg gcggcgaagg
     2521 tgcgcaccac atcggcggcg ctgcgtccgg tggattcttg cagccggttg acgaaggacg
     2581 gcccgccgcg attgaccagg tcgttggcga cgacacgggc gatgatttcg cggcgtagtc
     2641 gatggccgtg gatttcggcg gtgtatttct tcgccatccg ctccggaaaa tagcccatca
     2701 ggtcgcggtc gaaatgcgga tcgtccggca cgtcggaagc gacgatatcg gaaaacagta
     2761 cgatcttggc ataggccagt agcacgccga gttcagcgcg cgtcagcggt tcgccgcgtg
     2821 cctcgcgctc ggcaaggccg gccggcgagg gcagggtttc gacggtccgg ttgagcagac
     2881 cgcgcgtttc cagcgcttcc atgaagcggc cctgatggac gatgtcggtc agtccgcgct
     2941 tgcgcgccag cgaaagggcc agcgtctgct cgtaattgtt ggcgagcacc aggtggccga
     3001 cctcgtcggt catgtcggcc aaaagcttgt tgcgcgcggg ccgggtcagc gaacccttgc
     3061 gcatggcgga ggccagcgcg atcttgatgt tgacctcgac gtcggagcag ttgacgccgc
     3121 cggaattgtc gatggcatcg gaattgcaca ggccgccctt caggccgaat tcgatgcggg
     3181 cgcgctgggt gacgccgaga ttggcgcctt cgccgatcac cttggcgcgc acgtcgagcg
     3241 cggtgaggcg gatggcgtcg ttggcgcggt cgccgacttc ctggttggtc tcggtggtgc
     3301 cgcgcagata ggtgccgatg ccgccgaacc acaaaaggtc gaccggggcg gccaggatgg
     3361 cgcgcatgat ttccgttggc gaggccaccg tcttgccgag gccgatggcg gcggcggcgg
     3421 cttgcggcag ggtgatcgac ttctggctgc gcgagacgat gacgccgccc gccgacagct
     3481 tcgacttgtc gtaatcctgc cagctcgaac gcggcaggtc gaacaggcgc tggcgctcgg
     3541 caaaggaggc ggccatgtcg ggatcggggt cgatgaagat gtcgcggtgg tcgaaggcgg
     3601 cgatcagccg ggtctcttgc gagcgcagcg cgccgttgcc gaacacgtca cccgacatgt
     3661 cgccgacgcc ggccatggtg aagggcgtgg cctggatatc gcggttcatc tcgcggaaat
     3721 gccgcttcac cgcttcccat gcgcccttgg cggtgatgcc catcttcttg tggtcgtagc
     3781 cggcggaacc accggaggcg aaggcgtcgt ccagccagaa atcatgcgcc tggctgatgg
     3841 cgttggcggt gtcggagaag gtggcggtg
//
LOCUS       DASYCS010000099         3204 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1178321,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000099 DASYCS010000000
VERSION     DASYCS010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178321"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..155
                     /locus_tag="VGM46_03540"
     CDS             <1..155
                     /locus_tag="VGM46_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006204878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AzlD family protein"
                     /protein_id="HEY4191695.1"
                     /translation="TTLVAPSILTAGPAEWAALIVTGFASLRGGLMTMFLAGAAVLII
                     CRQFVG"
     gene            complement(152..1990)
                     /locus_tag="VGM46_03545"
     CDS             complement(152..1990)
                     /locus_tag="VGM46_03545"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEY4191696.1"
                     /translation="MFQTFDTAGDPLAGKPRVGKLREWLVKNGLDGFIVPRADEHQGE
                     YVPARAARLKWLTGFSGSAGAAIVLKDRAMIFVDGRYTLQVRNEVDLDVFTVESLVDN
                     PPPSWIAGNLAKGARLGFDPWLHTLSEAKALRAAAEKAGATLLPLDHNPVDAIWKDQP
                     ASPLEPVEIHPNALAGELAKDKLARLAADIGKAGATHAILTDPSSLAWAFNIRGGDVP
                     HTPLALGFAVLAADGNHHIFMDRRKFSRTVAAYLTQLAELHAPGEFETAVAELARTGA
                     KIALDPALAAEKLKMLVEANGGTVIAAADPARIPRATKNDAEIAGSRAAHRRDGAAMA
                     KLLCWLDEQEPGSVDEIAVVKQLEESRRKIGDETQMPLRDISFDTISGAGPNGAIIHY
                     RVSRATTRKLGDGELFLLDSGAQFQDGTTDITRTIAIGQPSEEMRERFTLVLKGMIGI
                     SMLRFPAGTRGSEIDAVARMALWKHGCDFAHGTGHGVGSYLAVHEGPQRIARTGTEKL
                     LQGMILSNEPGYYKEGVYGIRIENLILVTPAETIEGGDMPMHAFETLTLAPIDKRLIR
                     TDLLAREELQWLDAYHARVLAEIGPMVDGQTLAWLVKATAPLAHVA"
     gene            complement(2213..3085)
                     /locus_tag="VGM46_03550"
     CDS             complement(2213..3085)
                     /locus_tag="VGM46_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006204880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEY4191697.1"
                     /translation="MTQTRLHFTATKADAERAYAAFEPAFEDDGLPLAITEIDEDRDI
                     HEISLYAGDDVDAVEQRMGDLLAGISLSKPIEREILPDIDWVTRSLEGLKPVRAGRFF
                     VHGAHDRDQLRQGDIAIEIEAGLAFGTGHHGTTAGCLEMLESVVEAEHPENALDLGTG
                     SAVLAIALAKLAHIPVLATDIDPIAVEVAAENVRLNGTADLVETVTATSFDHPVFKQR
                     GPFDLIVANILAGPLMEIAPDTAAHLKPGGSLILSGILDRQHDAVVEAYVAAGFAHIR
                     TTHLEGWVTIHLKR"
     gene            complement(3097..>3204)
                     /locus_tag="VGM46_03555"
     CDS             complement(3097..>3204)
                     /locus_tag="VGM46_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015317227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEY4191698.1"
                     /translation="SVLLLNSKGDFAGTIAYGENPKNAVAKLTRLAAEG"
BASE COUNT          521 a          981 c         1079 g          623 t
ORIGIN      
        1 tgacaacgct ggtcgcgccc tccatcctga cggcgggtcc cgccgaatgg gcagcgttga
       61 tcgtcaccgg gttcgcctcg ttgcgcggcg ggctgatgac gatgttcctg gcgggggccg
      121 cggtgctgat tatctgccgg cagttcgtcg gttaggcgac gtgcgccagc ggcgctgtcg
      181 cctttaccag ccaggcgagc gtttggccat cgaccatcgg tccgatctcg gccagcacgc
      241 gggcgtggta ggcatccagc cattgcaatt cttcgcgcgc gagcaaatct gtgcggatga
      301 gccgcttgtc gatgggcgcc agcgtcagcg tctcgaaggc gtgcatcggc atatcgccgc
      361 cctcgattgt ctctgctggt gtgaccagga tcagattctc gatgcggatg ccgtagacgc
      421 cttccttgta gtagccgggt tcgttggaaa ggatcatgcc ttgcagcagc ttttccgtgc
      481 cggtgcgggc gatgcgctgg gggccttcat gcaccgccag ataggaaccg acgccgtggc
      541 ctgtgccgtg ggcgaagtcg cagccatgct tccatagcgc catgcgcgcc accgcatcga
      601 tttccgagcc gcgcgtgcct gccgggaacc gcagcatgga aatgccgatc atgcctttca
      661 gcacaagggt gaagcgttcg cgcatttcct cgcttggctg accgatcgcg atggtgcggg
      721 tgatgtcggt ggtgccgtcc tggaactgcg cgccggaatc gagcaggaac aattcgccgt
      781 cgcccagctt gcgggtggtg gcgcgcgaga cacggtagtg gatgatggcg ccgttggggc
      841 cggcgccgga aatggtgtcg aaggaaatgt cgcgcagcgg catctgcgtt tcgtcgccga
      901 tcttgcggcg gctttcctcc aactgcttta ccacggcaat ttcatcgacg ctgccgggtt
      961 cctgctcatc caaccagcac agaagcttgg ccatcgccgc accgtcgcgg cggtgcgcgg
     1021 cgcgggaacc ggcgatttcg gcgtcgttct tggtagcgcg gggaatgcgc gccgggtcgg
     1081 cggcggcgat caccgtgccg ccattggcct cgaccagcat tttcagcttt tcagcggcaa
     1141 gcgccgggtc gagcgcgatt ttcgcgccgg tcctcgccag ttcggcgacc gccgtttcaa
     1201 attcgcccgg cgcatggagt tcggcaagct gcgtgagata ggcggcgacg gtgcgcgaga
     1261 atttacgcct atccatgaag atgtggtgat tgccatcggc ggcaagcacg gcgaaaccga
     1321 gcgccagcgg cgtatgcggc acgtcgccgc cgcgaatgtt gaaggcccag gcgagggagg
     1381 atgggtcggt gagaatggcg tgggtcgcgc ccgcctttcc aatgtctgcc gccaaccgcg
     1441 ccagcttatc cttggccagt tcgccggcaa gcgcgttcgg gtggatttcg acaggttcga
     1501 gcggcgaggc cggctggtct ttccagatgg cgtcgaccgg gttgtggtcg agcggtagaa
     1561 gggttgcgcc ggccttttcg gcggctgcgc gcaacgcctt cgcctcgctt agcgtgtgca
     1621 gccaggggtc gaagcccagc cgcgcgccct tggccaaatt gccggctatc cacgatggcg
     1681 gcgggttgtc gacgaggctt tcgacggtga agacgtcgag gtcgacctcg ttgcgcacct
     1741 gaagggtgta gcggccatcg acaaagatca tggcgcgatc cttcaagacg attgccgcgc
     1801 ccgcagaccc ggaaaagccg gtcagccatt tcagccttgc cgcgcgtgcc ggaacatatt
     1861 cgccctgatg ctcgtcggcg cgcgggacga tgaagccgtc cagcccgttc ttcaccagcc
     1921 attcgcgcag cttgccgaca cgcggcttgc cggccaaggg atcgccggcg gtatcgaaag
     1981 tctggaacat gcggaagacg cctttgccga atttgttatg cgcgaaacta gcggcctggc
     2041 caacggctga caatcggcgg cgtgtgggcc agcgacgtaa tcgccctggg ttccccacgc
     2101 ctggcccctc cctacaaggg gaggtggatt cttccgcaac cggccgttcg taatccgccg
     2161 ggctacgtgc cggttgccgc cagattccct tccggttcgt agggaggggg tttcagcgct
     2221 tgaggtggat cgtcacccag ccttcgagat gagtggtgcg gatatgagcg aagcctgccg
     2281 cgacataagc ttccaccacc gcatcgtgct ggcggtcgag aatgcccgac aggatcaacg
     2341 agccgccggg cttcaagtgc gccgccgtat cgggtgcgat ctccatcagc ggcccggcaa
     2401 ggatgttggc gacgatcagg tcgaacggcc cgcgctgttt gaagaccggg tggtcgaagc
     2461 tggtggcggt gaccgtctcg accaggtcgg ccgtgccgtt caggcggaca ttttccgccg
     2521 ccacttccac ggcgatgggg tcgatgtcgg tcgccagcac cggaatatgg gcaagcttgg
     2581 ccagcgcgat ggccagcacg gcgctgccgg tgccgaggtc gagcgcattt tcaggatgct
     2641 cggcctcgac cacgctttcc agcatttcga ggcaaccggc ggtggtgccg tgatgtccgg
     2701 taccaaaggc gaggcctgcc tcgatctcga tggcgatgtc gccttggcga agctggtcac
     2761 ggtcatgggc gccatgaacg aaaaagcggc cggcgcgcac cggcttcagg ccttccagcg
     2821 agcgcgtgac ccaatcgata tcgggcagga tttcgcgctc gatcggcttc gacagggaaa
     2881 tgccggcgag aagatcgccc atgcgctgct cgaccgcatc gacgtcgtcg ccggcataga
     2941 gcgagatttc gtggatatcg cggtcctcgt cgatttccgt tatcgccagg ggcaaaccgt
     3001 catcctcgaa agccggttca aacgccgcat aggcgcgttc ggcgtcggcc ttcgtggcgg
     3061 tgaaatgaag tctggtctgc gtcatcgaga tgcccgtcag ccttccgccg caagccgggt
     3121 tagcttggcg acggcatttt tcggattttc tccataggcg atagtgccgg caaagtcgcc
     3181 cttgctgttc agcagcagca ccga
//
LOCUS       DASYCS010000100         2041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3065510,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000100 DASYCS010000000
VERSION     DASYCS010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3065510"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VGM46_03560"
     CDS             <1..192
                     /locus_tag="VGM46_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY4191699.1"
                     /translation="RPDSPSVRLHERLGFHHSGRLEGSGYKHGSWLDTVFMQLEMNGG
                     ASSSPDPQSLPERRFRQRA"
     gene            complement(315..695)
                     /locus_tag="VGM46_03565"
     CDS             complement(315..695)
                     /locus_tag="VGM46_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2794 domain-containing protein"
                     /protein_id="HEY4191700.1"
                     /translation="MTDGGSEGGDASGNLIPLHEARRERLELPVAFERRELEEILRLY
                     GRMVAANEWRDYAIDHLSDRAVFSVFRRASEVPLFQIVKDPKLARKQGAFSVIAAGGR
                     ILKRGHELTRVLGIFDRTLKLVEA"
     gene            1246..>2041
                     /locus_tag="VGM46_03570"
     CDS             1246..>2041
                     /locus_tag="VGM46_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4175 family protein"
                     /protein_id="HEY4191701.1"
                     /translation="MPDLPAIEPARPSSRLATSRLVTSGSMIVERGWPLLLPLVLVGA
                     LFLSISWFGLFPLMPDALRMGVLAIFGLAGLAALYPLRFFRLPKPGEVDRRIEAANAL
                     PHSPVQVQSDRPSGAETGFSQALWREHQKRMAATLGHLGSDLPRTRVPERDPWGLRAL
                     AALALAVAFAFSFGSLGGSVGDGFRPAATRDAAPPRIDAWVTPPVYTGKPPVFLAAGA
                     ADSAAISIPVGSDLSLRVTGGSGDETLAYADAGGNSRELDPSATPTA"
BASE COUNT          349 a          686 c          608 g          398 t
ORIGIN      
        1 cggcccgaca gcccatcggt aaggctgcat gaaaggctgg gctttcatca cagcggcagg
       61 ctggaaggct ccggctacaa gcacgggagc tggctggata cggttttcat gcagttggag
      121 atgaatggcg gcgcgtcgtc gtcgccggac ccgcaatcat tgccggaacg ccggttcagg
      181 caaagggctt agagcatttc cagcaaaaag tgtaaaacag ttttgcgtcc agaaatgcgg
      241 ccaaaacaaa gagatggagt gattttgtcg atccggtaaa aatcggaacc gctctagcag
      301 gcggcgttgc ggcttcaggc ttcgaccagt ttcaaggtac ggtcgaaaat gccaagcacc
      361 ctggtcaatt catggccgcg cttgaggatg cgcccgccgg cggcgatcac gctgaaggcg
      421 ccctgcttgc gcgccagctt cgggtccttg acgatctgga acaaaggcac ctcgctggcg
      481 cggcgaaaca ccgagaacac ggcacggtcc gacaagtggt cgatggcgta gtcgcgccac
      541 tcattggcag ccaccatgcg cccgtacagc ctcaatattt cttcaagctc gcgccgctcg
      601 aacgcgaccg gcagttcaag ccgttcgcgg cgggcctcat gaagcggaat caaattcccc
      661 gatgcgtccc cgccctccga accaccatcg gtcatgccaa gcctcgcaaa ttggtatctt
      721 caccaaggtt gcgctttcgt tggctaattg caagcccgcc gcgattccgc gcgcccgcgc
      781 cctatctcga tcgtccagtc acgttttcaa acgcggctgt tggaattggt gattccttgc
      841 cgcattttgg ccgtttttta tccgcgctac cgccccaatg tcccttgaac ttgcccgtgt
      901 tgcctgagac ggttcggtcc ggcgcctgct cgtaaacccc aagccccccg cccacgggcc
      961 tgagtcggat cgaaccaacc tcggtctttt ttagaaagcc aacctggttt tttcaaaaga
     1021 tcgggtgcag caatttaacg accttgaatg aacggcgttg gcccctgcca gcgccgtttt
     1081 tttattccgc atccgcgcaa taccacgggc atcggccagc cgcgacctcc ggcatttgag
     1141 cgggcattat cgcccaacgc ctcgcctttg ccgtgtttgc tgctatcctg acctgaatgg
     1201 ccggccggct tggccggcga accgtaaaat ggaacgaaag ggctgatgcc ggacctgcct
     1261 gccattgaac ccgcaaggcc ttcttcgagg ctggcgacca gccgtctggt cacaagcggc
     1321 tcgatgatcg tcgagcgcgg ctggccgttg ctgttgccgc tcgtgctggt tggcgcactt
     1381 ttcctcagca tatcctggtt tggcctgttc ccgctgatgc ccgacgccct gcggatgggc
     1441 gtcctcgcca tattcggcct tgccggactt gcagcactct atccgctgcg cttcttccgc
     1501 ctgccgaagc caggcgaagt cgaccgccgc atcgaagcgg caaacgccct gccgcacagc
     1561 ccggtccagg tacagtccga ccggccaagc ggggccgaga ccggcttttc gcaggcgctg
     1621 tggcgcgagc accagaagcg catggcggca acgcttggcc atcttggctc ggacctgccg
     1681 cgcacccgcg tgcccgagcg cgacccgtgg ggcctgcgcg cgctggccgc cctggcgctt
     1741 gccgtcgcct tcgccttctc gtttgggtcg ctgggcggca gcgtgggcga cggtttccgt
     1801 cccgctgcga cgcgcgacgc cgcgccgccg cgcatcgatg cctgggtgac gccgccggtc
     1861 tataccggca agccgccggt cttcctcgcc gccggagcgg ccgacagcgc cgccatttcc
     1921 atcccggtcg gcagcgacct gtcactgcgc gttaccggcg ggtccggcga cgagacgctc
     1981 gcctatgccg acgccggcgg caacagccgc gagcttgatc cttccgcgac accgacggcc
     2041 g
//
LOCUS       DASYCS010000101        14032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_166188,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000101 DASYCS010000000
VERSION     DASYCS010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 14032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14032
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166188"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="VGM46_03575"
     CDS             complement(<1..523)
                     /locus_tag="VGM46_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEY4191702.1"
                     /translation="MKIGFVCAVDVGTGSARAGILDAAGTLLGRAEKPIAMNRPKADF
                     AEQDSQDIWAAVCAAVRLAREKAGVDPAAVVGIAFDATCSLVVRDREGGQLSVSTCGE
                     KRWDTICWLDHRAIAEADECTRSGHEVLDYLGGVMSPEMQTPKLMWLKRNLPATWRQA
                     GYMFDLTDFLTWKA"
     gene            complement(662..2140)
                     /locus_tag="VGM46_03580"
     CDS             complement(662..2140)
                     /locus_tag="VGM46_03580"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006325938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEY4191703.1"
                     /translation="MTAKLSLASLSSLPVTIARPSYDRSALKAGIMHFGVGNFHRAHQ
                     AVYLDDLFATGIGHDWALVGAGVFEGEKIGREKLQEQDWLTTVVEQDEGHMSARVTGA
                     MVDFITPGDAQAIIERLAEPAIRIVSLTITEGGYFIDPASGVFNPTHPDIVADAQNPS
                     APKTVFGIILAGLVRRRADGIVPFTIMSCDNIPHNGHVTSDGVVGLARLIDMDLANWV
                     SEHVAFPNSMVDRITPATSDRERSILAKEFGLDDNWPVFCEPFRQWVLEDRFTGGRPP
                     LEKVGVQFVRDVAPYELMKIRILNGGHATIAYPAGLMDIHFVHEAMQEPLVRGFLDKL
                     EHDEIIPTVPPVPDTSLKDYYRLIDKRFSNPKIGDTVRRLCLDGSNRQPKFIIPTIAD
                     RLKAGKGVSGLALESALWCRYCFGATDSGAIIEPNDPSWGRLQATAKAAREAPAAWLG
                     MEDIYGEVGRAPAFTEAFAHALNALWADGARATVTRYLAGTL"
     gene            complement(2340..3110)
                     /locus_tag="VGM46_03585"
     CDS             complement(2340..3110)
                     /locus_tag="VGM46_03585"
                     /EC_number="1.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-iditol 2-dehydrogenase"
                     /protein_id="HEY4191704.1"
                     /translation="MRLAGKSALITGSARGIGRAFAEAYVREGATVAIADINLEAAQK
                     TAAEIGDKAHAVKLDVTDLASIEAAVKAVEDKTGGLDILINNAALFDAAPIVEIGKAS
                     YEKLFSVNVAGTLFMMQAAARSMIARGQGGRIINMASQAGRRGEALVAVYCATKAAVI
                     SLTQSAGLDLIKHRINVNAIAPGVVDGEHWDGVDALFAKYENRPRGEKKKLVGEAVPY
                     GRMGRADDLTGMAVFLASTEAEYIVAQTYNVDGGQWMS"
     gene            complement(3112..4110)
                     /locus_tag="VGM46_03590"
     CDS             complement(3112..4110)
                     /locus_tag="VGM46_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006201821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4191705.1"
                     /translation="MGNITLKNVSKSFGSTVIIPGIDLVIENGEFVVFVGPSGCGKST
                     LLRLIAGLEDTSGGTINIDGRDVTGEAPAKRKLAMVFQSYALYPHMSVAKNIAFPLKM
                     AGESQATIEKKVSDAARVLNLTNYLERRPGQLSGGQRQRVAIGRAIVRNPGAFLFDEP
                     LSNLDAALRGTMRLEISELHQQLKTTMIYVTHDQVEAMTMADKIVVLNAGNIEQVGSP
                     MELYKTPANLFVAGFIGSPKMNLIEGAPAAKHDARTIGIRPEHLKISTTAGEWKAKVG
                     VAEHLGSDTFLHVQTDEVGPLTVRADGEMDVHHGDTIYLTPDQTKLHRFGPDGKAI"
     gene            complement(4142..4966)
                     /locus_tag="VGM46_03595"
     CDS             complement(4142..4966)
                     /locus_tag="VGM46_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014329271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY4191706.1"
                     /translation="MARAVSTRAKIGWTIAAWAVALLIFFPILYTIITSFKSETEAIA
                     GFSLIPSGTLESYSEVQSQYNYFKPFMNSVIISVGSTLIALAVAIPAAWSMAFSPTKH
                     TKDILMWMLSTKMMPAVAVLVPMYLIFRDWNLLDSRIGLTIMLMLINLPIVVWMLYTY
                     FREIPGEILEAARMDGATLWGEIVYVLTPMAVPGIASTLLLNIILAWNEAFWTIRLTT
                     TEAAPLTAFITSFSSPQGLFWAKLSAASTLAIAPILIMGWFSQKQLVRGLTFGAVK"
     gene            complement(4977..5849)
                     /locus_tag="VGM46_03600"
     CDS             complement(4977..5849)
                     /locus_tag="VGM46_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018326775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY4191707.1"
                     /translation="MATRQTGTLARFMMAPSVILLLIWMVVPLAMTLWFSFQNYNLLD
                     PTQTGFAGLFNYRFFYSDPAFFQSIWNTLVIVVGVLLITVIGGIALALLIDQPIFGQG
                     LVRILVISPFFVMPPVAALVWKNMIMHPQYGVFADIAKFFGLQPIDWFGQHPLLSIVI
                     IVAWQWLPFATLILLTALQSLDGEQKEAAEMDGAGFVSRFIHLTLPHMARAITVVILI
                     QTIFLLSVYAEILVTTNGGPGYASTNLPFLIYRTALLGYDVGGASAGGIIAVVLANIV
                     ALFLMRAVGKNLDK"
     gene            complement(5942..7252)
                     /locus_tag="VGM46_03605"
     CDS             complement(5942..7252)
                     /locus_tag="VGM46_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018326774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEY4191708.1"
                     /translation="MKLRTLILGLCSASALAFAANAESITIATVNKGDMVRMQKLTDD
                     FTAKNPDIQLQWVTLEENVLRERVTTDIATKGGQYDVVTIGNYEVPIWAKQGWLLSLD
                     KLGADYDVDDILPAIRGGLSLDGKLYAAPFYGESAMIMYRTDLMEKAGLKMPEKPTWE
                     FIGDAARKMTDRANGINGICLRGKAGWGENMAFLTAMDNSFGGRWFDEKWQPQFDQPE
                     WKNTLDTYVKLMKDAGPEGASSNGFNENLTLFQQGKCGMWIDATVAASFVSDPKNSTV
                     ADKVGFAIFPDTGNGNHGNWLWSWNLAVPASSQKADAAQKFVSWATSKGYTDLVASKE
                     GWTNAPPGTRTSLYNNEEYKKAAAFAGPTLAAMNAADITKPSVKPVPYTGGQFVAIPE
                     FQGLGTTVGQLFSAALAGQSSVDDALAAAQTAATREMTRAKYIK"
     gene            complement(7381..8337)
                     /locus_tag="VGM46_03610"
     CDS             complement(7381..8337)
                     /locus_tag="VGM46_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015314494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEY4191709.1"
                     /translation="MNARPESNSRLDDAARAGWLYYVAGHTQDQIAAALGISRQTAQR
                     LVSLAMSEGLIKVRVDHPIANCLDLAARLKSRFALDLVEVAPSVSGSSSTTIGVAEMA
                     AAQIEKWLRSPEPIVMGIGTGRTLKAAIGQLPPMECPQHKIVSLTGNIAPDGSAAFYN
                     VIFTMADRIKARSFPMPLPVIASSPAEREMLLSQPMIQQTLALAAQASVTFVGIGDLG
                     PKAPLYEDGFITESELKALQKAGAVAEIVGWVFDREGRPIDGETNDRVSSAPLPSREK
                     SLVVALAMGELKLPGILAAVNRRLVNGLITDEWTAAALLEAV"
     gene            8548..9180
                     /locus_tag="VGM46_03615"
     CDS             8548..9180
                     /locus_tag="VGM46_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEY4191710.1"
                     /translation="MTPKAVFWDMDGTLTDSEPLHEAALIAALHSVGVVPPADLHERV
                     LGVSAWPVYEMLRDEHGLSLPFDEWIVRKYDHYLPLVETLKPRPGAIEIFNELRERGV
                     AQAVVSNSDRMVVDANLRAVGLAYPGMRTVSRNDVREGKPHPEPFLRAAWLAGIDPAQ
                     AVAVDDSSTGATAALAAGMKTIFWPEKPMPGPAGAINLNDAAELRAELGL"
     gene            9283..11049
                     /locus_tag="VGM46_03620"
     CDS             9283..11049
                     /locus_tag="VGM46_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010914433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY4191711.1"
                     /translation="MAINLDALMNSSHLRGEGDGQTFIAPLDGMAHVSGDRTAPLLHK
                     TLPQLLAETVDAHGERDAAIFPSQDKRFTWNELSETIDAFAAGLLSLGLEKGDRIGIW
                     SPNRWEWLVTQFASARIGLILVNINPAYRLTELKYALNKVGCKALVTAVAFKSSDYLG
                     MLAALAPELESAEAGRLEAAALPALRIVIQMGEEIVPGMANFGDVLAMGGMDEYERLD
                     AISDGLDPDDAINIQFTSGTTGTPKGATLTHHNVINNGNFVTSAIKLTADDRLCIPVP
                     FYHCFGMTMGAVGGVSKGAAMVFPGEGFDPGVVLKAVADERCTAIYGVPTMFVAMLDH
                     PDFASFDLTSLRTGIMAGSPCPIEVMKKVVSLMHMSEVTIAYGMTETSPVSFQSNVDD
                     PLEKRVSTVGRVQPHVEVKIVDAGGATVRVDEAGELCTRGYCVMKGYWQDEEKTREAI
                     DEDGWMHSGDLATLDAEGYCNIVGRVKDMVIRGGENIYPREVEEFLYRHPKIKEVQVF
                     GVPDEKFGEALAAWIVLAPGNSATAEEIKAYCQGQISHYKIPLYIRFKESLPMTVTGK
                     PQKFVMRQAMIDELGLQPARTA"
     gene            complement(11115..11792)
                     /locus_tag="VGM46_03625"
     CDS             complement(11115..11792)
                     /locus_tag="VGM46_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010914441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP family channel protein"
                     /protein_id="HEY4191712.1"
                     /translation="MKTYLAEVLGTFLLVFIGTASVVTGGFGGALPVGQVGIGLAFGI
                     GLIAAAYAIGPISGAHLNPAVTLGVFLAGRMPAKDVIPYWIAQIIGAILASLALWVIV
                     AGQVGGHTTGFAPNGWDPAAYGTSSAFLAEVIATFTFVMVILGVTSAKHTTAFAGLVI
                     GLTLAALHFAFIPVSGNSLNPARSIGPALFTGGLALSQLWLYIVAPLIGGAIAGVVAR
                     TNMFEKD"
     gene            11956..13245
                     /locus_tag="VGM46_03630"
     CDS             11956..13245
                     /locus_tag="VGM46_03630"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010914442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEY4191713.1"
                     /translation="MRLGGRLAAAIEVLEDIGRRHRPVADALKDWGLSHRFAGGGDRA
                     AIGNIVYDALRRRRSAAWLVGEETPRAIGFGALLLDGGRTVAALNQALDGDKFAPAPL
                     SAVEIAAIEARRLTDAPDEIRADVPGWCVPLFKEAFGDDWAAEGAALAARPPLDIRVN
                     TLRAKRESVLAELAQAGAEPTLLAPHGIRIAPIDGDGRHPNVQAEPAFQKGWFEVQDE
                     GSQLVAELSGARAGMQVLDYCAGAGGKTLALSAAMDNHGQIFAHDAEKARLAPIFDRM
                     RRSENRNVQVVSKPSELAPLSAHMDVVLVDAPCTGSGTWRRRPDAKWRLTQRQLDARK
                     AEQASILDAAMAYVKPGGLLVYITCSVFEEENAAQTAAFLSRAPAFSPADHAALWRHH
                     FPGHEASARIDDDAGISLSPARSGTDGFYFCAMRKAV"
     gene            13344..13796
                     /locus_tag="VGM46_03635"
     CDS             13344..13796
                     /locus_tag="VGM46_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019859998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HEY4191714.1"
                     /translation="MKRSTSLLLFLVSVVSAGLLIGYATLPGEWYAGLAKPPFNPPNW
                     IFGPTWSLLYVLIAIAGWRTWRRSRSDLTMKLCGAQMLLNFLWSPVFFGAQQPGPALI
                     VIVALLAAIVGFIIVSWNHDRVSAWLFVPYAAWVAFATLLNASIWWLN"
     gene            13884..>14032
                     /locus_tag="VGM46_03640"
     CDS             13884..>14032
                     /locus_tag="VGM46_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein"
                     /protein_id="HEY4191715.1"
                     /translation="MAAKIVPAPDYEERVRASFLRQQAMATIGAELTLVTPGIVEIEM
                     AYSAKL"
BASE COUNT         2598 a         4280 c         4544 g         2610 t
ORIGIN      
        1 ttgccttcca ggtgagaaaa tcggtcaggt cgaacatata gcccgcctgc cgccatgtag
       61 cgggcaggtt gcgcttcagc cacatcagct tcggcgtctg catttccggc gacatgacgc
      121 cgccgagata gtcgagcacc tcatgcccgc ttcgggtgca ttcatccgcc tcggctatgg
      181 cgcggtggtc gagccaacag atggtgtccc aacgcttttc accacaggtc gaaacgctga
      241 gctggccgcc ttcacggtcg cgcaccacca gcgaacaggt ggcgtcgaaa gcgatgccga
      301 cgacagccgc cggatcgacg ccggcttttt cgcgcgccag gcggaccgcg gcgcagacgg
      361 ccgcccagat atcctgcgag tcctgttcgg cgaaatcggc cttcggccgg ttcatggcga
      421 tgggcttttc ggcgcgaccg agcagcgttc ccgcggcatc gaggataccg gcgcgggcgc
      481 tacccgttcc aacatcgacc gcgcagacga atccgatttt caagaagctt ttctcgctca
      541 atgccgcgtt gcggtatcca gcgagactat aaggggcgcg ccaaagggcg caagccggat
      601 ggatcttctg acccgccggc tatgcggcca aggttcgaac caatggaggc aggccgaacg
      661 gtcagagcgt gccggccaga tagcgcgtca cggtcgcgcg cgcgccgtct gcccacagcg
      721 cattgagcgc atgggcaaaa gcttcggtga aggccggcgc acggccaacc tcgccataga
      781 tatcctccat gccaagccag gcggcgggcg cttccctggc cgccttggcg gtagcctgca
      841 accggcccca actcggatcg ttcggctcga taatcgcgcc ggaatcggtg gcgccaaagc
      901 agtaacggca ccagagcgcc gattccagcg ccaggccgga cacgcccttg ccggctttca
      961 gccggtcggc aatggtcggg atgatgaatt taggctggcg gttagagccg tccaggcaaa
     1021 gccttcgcac ggtgtcgccg atcttcgggt tggaaaagcg cttgtcgatc aggcggtagt
     1081 agtctttcag gctggtgtcc gggacaggcg gcacggtcgg gatgatctcg tcatgttcga
     1141 gcttgtcgag aaagccgcgc accagcggct cctgcatcgc ctcatgtacg aagtgaatat
     1201 ccatcaggcc ggccggatag gcgatggttg cgtggccgcc attgaggatg cgtatcttca
     1261 tcagttcata gggtgcgacg tcgcgcacga actgcacgcc gaccttttcc agcggcgggc
     1321 ggccaccggt gaagcggtct tcgagcaccc attgcctgaa aggctcgcag aacaccggcc
     1381 aattgtcgtc caggccgaat tccttcgcca gtatggagcg ctcgcgatcg gaggtagccg
     1441 gcgtgatgcg atcgaccatc gagttcggga aggcgacatg ctcgctcacc cagttggcga
     1501 gatccatgtc gatcagccgc gccaggccga cgacgccatc cgaggtgaca tggccgttat
     1561 ggggaatgtt gtcacaggac atgatcgtga acggcacgat accatcggcg cgcctacgga
     1621 ccagcccggc caggatgatg ccgaagacgg ttttcggcgc ggagggattt tgcgcatcgg
     1681 caacaatgtc gggatgggtt ggattaaaca cgccggaagc cggatcgatg aaataaccgc
     1741 cctcggtgat ggtcagcgag acgatcctga tcgccggttc ggccagccgc tcgatgatgg
     1801 cctgggcgtc gcccggcgtg ataaaatcga ccatggctcc ggtgacgcgg gcgctcatat
     1861 ggccctcgtc ctgctcgacc acggtggtca gccagtcctg ctcctgaagc ttttcacgcc
     1921 cgatcttttc gccctcgaac acgccagccc cgaccagcgc ccagtcatgg ccgatgccgg
     1981 tggcgaacag gtcgtcgaga tagacggcct gatgggcgcg gtggaaattg ccgacgccga
     2041 aatgcatgat gccggccttc agcgccgagc ggtcatagga cggccgcgcg atggtgacgg
     2101 gcagggaaga aagactggcc aaagagagtt tcgcggtcat tgagcaaaag ttcctcttgc
     2161 gatttcgaga gccgaccagt cgccccctct ccgtcgcgct gcgcgcgcca cctctgcccc
     2221 gctgcgtggc gaggaaagtc gcctggcgtg cagccggttt ttcctcaccc ccgtgaggga
     2281 gagaggtggc tcggcgaagc cgggagggag cagaggggct gatttcagca tacgcatcat
     2341 cagctcatcc actggccgcc atcgacattg taggtctggg cgacgatgta ttcggcctcg
     2401 gtgctggcga ggaacaccgc catgccggtc aaatcgtcag cccggcccat gcggccataa
     2461 ggcaccgctt cgccgaccag tttcttcttc tcgccgcgcg ggcggttttc atatttggca
     2521 aacagcgcgt cgacgccgtc ccagtgctca ccatcgacca cgcccggcgc aatcgcattg
     2581 acgttgatgc ggtgctttat gaggtccagc ccggctgact gggtcagcga gatcaccgcc
     2641 gccttggtgg cgcaatagac agccaccagc gcctcgccgc gccggcccgc ctggctcgcc
     2701 atgttgatga tcctgccgcc ctgcccgcgc gcgatcatgg aacgcgccgc cgcctgcatc
     2761 atgaacagcg tgccggcgac attgaccgag aacagttttt cgtagcttgc cttgccaatc
     2821 tcaacgatgg gggcggcatc gaagagcgct gcgttattga tcagaatatc gaggccgccg
     2881 gtcttatcct cgaccgcttt caccgccgct tcgatggagg caaggtcggt aacgtcgagt
     2941 ttcaccgcat gggccttgtc gccgatttcg gctgcggttt tctgcgcggc ttccagattg
     3001 atatcggcga tggcgaccgt tgcgccctcg cgcacatagg cctcggcaaa cgcccggccg
     3061 atgccccgcg ccgaaccggt gatcagcgcc gattttcctg caaggcgcac gtcagatcgc
     3121 cttgccgtcg ggaccgaagc gatgcagctt ggtctggtcc ggcgtcagat agatggtgtc
     3181 gccatggtgt acgtccatct cgccatcggc gcgcacggtc agcgggccga cctcgtccgt
     3241 ttggacatgc aggaaggtgt cggagccgag atgttcggcc acgccgacct tggctttcca
     3301 ttcgccggcg gtggtcgaga ttttcaggtg ttcgggccgg atgccgatgg tgcgcgcgtc
     3361 atgcttggcg gcgggcgcgc cttcgatcag gttcatcttc ggcgaaccga taaagccggc
     3421 gacgaacagg tttgccggcg tcttgtagag ttccatcggc gagccgacct gctcgatatt
     3481 gccggcattc aggacgacga tcttgtcggc catggtcatg gcttccacct ggtcgtgggt
     3541 gacgtagatc atggtggtct tgagctgctg gtgcagttcg gaaatttcca gccgcatggt
     3601 gccgcgaagt gcggcgtcga ggttggacaa cggctcgtcg aacaggaagg cgccggggtt
     3661 gcgcacgatg gcgcggccga tggcgacgcg ctggcgctgg ccgcccgaaa gctggccggg
     3721 ccggcgttcg agataattgg tgaggttcag cacgcgcgcg gcgtcggaca ccttcttttc
     3781 gatggtcgcc tggctttccc cggccatctt gagcgggaag gcaatgttct tggccacgct
     3841 catatgcggg tagagcgcat aggactggaa caccatggcc agcttgcgct tggccggcgc
     3901 ttcgccggtg acatcgcggc catcgatgtt gatggtgccg ccgctggtgt cttccagccc
     3961 ggcgatgagg cgcagcagcg tggacttgcc gcagccggac gggccgacga agacgacgaa
     4021 ctcaccattt tcgatgacca ggtcgatgcc ggggatgatg acggtcgatc cgaaggattt
     4081 cgacacgttc ttgagcgtga tgtttcccat gatttcctcc ccaaagggct tgtcggatcg
     4141 attatttaac ggcgccgaaa gtcaggccgc gcaccagctg cttctggctg aaccagccca
     4201 tgatcaggat cggtgcaatg gccagcgtcg aggccgccga aagcttggcc cagaacaggc
     4261 cctgcgggct agagaaggag gtgatgaagg cggtgagcgg cgctgcttcc gtcgtcgtca
     4321 ggcggatggt ccagaacgcc tcgttccacg ccaggatgat gttgagcagc agcgtcgagg
     4381 cgatgcccgg caccgccatc ggcgtcagca catagacgat ctcgccccac aaagtcgcgc
     4441 cgtccatgcg cgcggcttcc aggatttcgc ccggaatttc gcggaaatag gtgtagagca
     4501 tccacaccac gatcggcagg ttgatcagca tcagcatgat ggtgaggccg atgcggctgt
     4561 cgagcaggtt ccagtcgcgg aagatgaggt acatcggcac cagcacggcc accgccggca
     4621 tcatcttggt ggacagcatc cacatcaata tgtccttggt gtgcttggtc ggcgagaacg
     4681 ccatcgacca ggccgccggg atcgccaccg ccagcgcgat cagcgtggag ccgaccgaga
     4741 tgatcaccga gttcatgaac ggcttgaagt aattatattg cgattgcacc tcggaatagc
     4801 tttccagcgt tcccgacggg atgaggctga agccggcaat cgcttccgtt tccgatttga
     4861 acgaggtgat gatggtgtag agtatcggaa agaagatcag cagagcgacc gcccaggcgg
     4921 cgatggtcca gccgatcttg gcccgtgtgg agactgcgcg tgccatcggg tctctcctat
     4981 ttgtcgaggt tcttgccaac cgcgcgcatc aggaacagcg cgacgatatt ggccagcacc
     5041 accgcgatga tgccgccagc cgaagcgccg ccgacatcgt agccgagcaa agcggtgcga
     5101 tagatcagga atggcaggtt ggtcgaagcg tagccggggc cgccattggt ggtgaccagg
     5161 atttccgcat agacgctgag caggaagatg gtctggatga ggatgaccac ggtgatcgcc
     5221 cgcgccatgt gcggcagcgt caggtggatg aagcggctga cgaagccggc gccgtccatc
     5281 tcggcggctt ccttttgctc gccgtccagc gattgcagcg cggtgagcag gatcagcgtg
     5341 gcgaaaggca gccattgcca ggcgacgatg atgacgatcg acaatagcgg gtgctggccg
     5401 aaccagtcga tcggttgcag gccgaagaac ttggctatgt cggcgaagac gccgtattgc
     5461 gggtgcatga tcatgttctt ccacaccagc gccgccaccg gcggcatgac gaagaagggc
     5521 gagatgacca ggatgcgcac caggccctgg ccgaagatcg gctggtcgat cagcagcgcc
     5581 agcgcgatgc cgccgatgac ggtgatgagc aggacgccga cgacgatgac cagcgtgttc
     5641 cagatcgact ggaagaaggc cggatcgctg tagaagaagc ggtagttgaa gaggccggca
     5701 aagccggtct gcgtcgggtc gagcagatta tagttctgga acgagaacca cagcgtcatc
     5761 gccagcggca cgaccatcca gatcaggagg aggattaccg aaggcgccat catgaagcgc
     5821 gcaagcgtac cggtctgtcg agtagccatg gcgaataccc tgcatcggac agattgagtt
     5881 gttcatcgag ttgggtggcc gcccggatga gaccggacga ccaaggcgcc ggggcgccgc
     5941 tctatttgat gtatttggcg cgcgtcattt cgcgggtcgc cgccgtctga gcagcggcga
     6001 gcgcatcgtc aacgcttgac tggccggcaa gggctgccga gaacaactgg ccaaccgtcg
     6061 tgccgagacc ctggaattcc gggatcgcca cgaattgccc gccagtatag ggaaccggct
     6121 ttacgcttgg cttggtgatg tcggccgcat tcatggcggc gagcgtcggg ccggcgaagg
     6181 cggccgcctt cttgtactct tcattgttgt agagcgaggt ccgcgttccg ggaggagcgt
     6241 tggtccagcc ttccttggaa gcgacgagat cggtgtagcc cttgctggtc gcccatgaga
     6301 cgaacttctg cgcggcgtcc gccttctgcg aggaagccgg gacagccagg ttccacgacc
     6361 acagccaatt gccgtggttg ccgttgccgg tatcggggaa gatggcgaag ccgaccttgt
     6421 cggcgacggt ggaattcttc gggtccgaca cgaaggaagc cgccaccgtg gcgtcgatcc
     6481 acatgccgca cttgccctgc tggaacaggg tcaggttttc gttgaagccg ttggaggaag
     6541 cgccctccgg tccggcgtcc ttcatcagct tgacgtaggt gtcgagcgtg ttcttccatt
     6601 cgggctggtc gaactgcggc tgccactttt cgtcgaacca gcgaccgccg aaggaattgt
     6661 ccatggcggt caggaacgcc atgttctcgc cccagccggc cttgccgcgc aggcagatgc
     6721 cgttgatgcc gttggcgcgg tcggtcatct tgcgggccgc atcgccgatg aattcccagg
     6781 tgggcttttc cggcatcttc agcccggcct tttccatcag gtcggtgcgg tacatgatca
     6841 tggcggattc gccgtagaac ggcgcggcat aaagcttgcc gtcgagcgaa aggccgccgc
     6901 ggatcgccgg caggatgtca tcgacatcgt aatcggcgcc aagcttgtcg agcgagagca
     6961 accaaccctg cttggcccag atcggcactt cgtaattgcc gatggtgacg acgtcatact
     7021 ggccgccctt ggtggcgatg tcggtggtga cgcgctcgcg cagcacgttc tcttccagcg
     7081 tcacccactg aagctggatg tcggggttct tggcggtgaa gtcgtccgtc agcttctgca
     7141 tgcggaccat gtcgccctta ttgacggtgg cgatggtgat ggattcggca tttgcggcga
     7201 acgcaagcgc gcttgccgaa cacaggccga ggatgagcgt gcgcagtttc atgaatttcc
     7261 tcccgaaaac caaatgagca attgcctcaa tagtgagcaa ttattcagtc ggcatgaatt
     7321 ctgtcaagcg cattcgttgt tgcgccgcag cacgggggtc gcccgaaact gctgccgcgg
     7381 tcagaccgct tccagcaatg cggcggcggt ccattcgtcg gtgatcaacc cattgacgag
     7441 gcgtctgttc accgccgcga ggatgcccgg cagtttcaat tcgcccatgg cgagcgccac
     7501 gaccagcgat ttttcgcgcg agggcaacgg agccgacgag acgcggtcgt tggtttcgcc
     7561 atcgatcggg cggccttcgc ggtcgaatac ccagccgacg atttcggcga ccgcgcccgc
     7621 cttctgcaac gccttcaatt cactttccgt aatgaagcca tcttcataga gcggcgcttt
     7681 cgggccgagg tcgccaatgc cgacgaaggt gacgctggcc tgcgccgcaa gggccagcgt
     7741 ctgctggatc atcggctggc tgagcagcat ttcacgctcc gccggcgacg aggctatcac
     7801 cggcagaggc atcgggaagg agcgcgcctt tatccggtcg gccatggtga agatgacgtt
     7861 gtagaacgcg gctgagccat ccggcgcgat gttgccggtc agcgacacga tcttgtgctg
     7921 cgggcattcc atcggcggca attggccgat cgcagccttc agcgtgcgcc cggtgccgat
     7981 gcccatgacg atgggttcgg gcgagcgcag ccatttctca atttgggcgg ctgccatctc
     8041 ggcaacgccg attgtcgttg acgatgaacc ggaaacgctt ggcgcaactt cgaccaggtc
     8101 gagggcgaag cgggatttga gccgcgcggc aaggtcgagg caattggcga tcgggtgatc
     8161 gacgcgaacc ttgatcagcc cttccgacat ggccagcgag acgagacgct gcgccgtctg
     8221 gcgcgatatg cccagcgcgg cggcgatctg gtcctgggta tggccggcga cgtagtaaag
     8281 ccagccggcg cgcgccgcat cgtcaagcct gctgttgctc tctggccggg cattcatcat
     8341 gtgcttcctc tgccccgcat ttttgcagga attctttttg agagcaattg ctcgataaat
     8401 gtgtcagata ttcaagatgc cgcgccagtt tagggcaatc gaccggccaa atgcgagaat
     8461 cgatttgtct gccgcgcgaa aagctttatg gcgtcggggc caaggcgcaa ccggcattga
     8521 ctgcgccgcg atgaacggga gaatccgatg acgccgaagg cggtcttctg ggacatggac
     8581 ggcaccctta ccgacagcga gccgctgcac gaggcggcgc tgatagcggc gctgcacagc
     8641 gtgggcgtgg ttcctcccgc cgatcttcac gagcgcgtat taggcgtttc agcttggccc
     8701 gtctacgaga tgctgcgcga cgaacatggg ctgtcgctgc cattcgacga atggatcgtg
     8761 cgcaaatacg accattatct gccgctcgtc gaaactttga agccgcgccc cggcgcaatc
     8821 gagatcttca acgaattgcg tgagcgcggc gtggcgcagg ccgtggtttc caattccgac
     8881 cgcatggtgg tggacgccaa tctgcgcgcg gtcggccttg cgtatcccgg catgcgcacg
     8941 gtgagccgca atgacgtgcg cgagggcaag ccgcatccgg aacccttttt gcgcgccgca
     9001 tggctggccg gcatcgatcc ggcgcaagcc gtggcggtcg acgacagttc gacgggagcc
     9061 actgcggcgc ttgccgccgg catgaagacg attttctggc cggaaaagcc gatgcccggc
     9121 ccggccggcg cgatcaacct gaacgatgcc gccgaattgc gggccgaact cggcctctag
     9181 ctccaccagc cgtcccgccg atacaggctc gctgccgttt cgtagcggtt ttcacgccgg
     9241 tcgcgggctg ataggctccg gccatctgcg aaggaggccg ccatggcgat caatctcgat
     9301 gccttgatga attcaagcca tctgcgcggc gaaggcgacg gccagacctt catcgcaccg
     9361 ctggacggca tggcgcacgt ctcgggcgac cgaacggcgc cgctgctgca caaaaccctg
     9421 ccgcaattgc ttgccgagac tgtggatgcg catggcgagc gcgacgcagc gatttttccg
     9481 agccaggaca agcgctttac ctggaacgaa ctgtcggaaa ccatcgatgc gtttgccgcc
     9541 ggcctgctct cgctggggct tgaaaaaggc gaccgcatcg gcatctggtc gccgaaccgc
     9601 tgggagtggc tggttacgca gttcgccagc gcccgcattg ggctgatcct cgtcaacatc
     9661 aacccggcct accggctgac cgaactgaaa tatgcgttga acaaggtcgg ctgcaaggcg
     9721 ctggtcaccg ccgtcgcgtt caagtcgtcc gactatctcg gcatgttggc ggcgctggcg
     9781 ccggaactgg aaagtgccga ggcaggcagg ctggaggcgg ccgctctgcc ggcgctgcga
     9841 atcgtcattc agatgggcga ggaaatcgtg cccggcatgg ccaatttcgg cgatgtgctg
     9901 gccatgggcg gcatggacga atatgaacgc ctcgacgcga tttcagacgg gcttgacccg
     9961 gacgacgcca tcaacatcca gttcacgtca ggcacgaccg gcacgcccaa aggagcgacg
    10021 ctcacccatc acaatgtgat caataacggc aatttcgtca cctccgccat caaattgacc
    10081 gcagacgacc ggctttgcat cccggtgccg ttctaccatt gcttcggcat gacgatgggg
    10141 gcggtcggcg gcgtcagcaa gggggcggcg atggtgtttc cgggcgaagg tttcgacccc
    10201 ggcgtggtgt tgaaggccgt cgccgacgaa cgctgcaccg cgatctacgg cgtgccgacc
    10261 atgttcgtgg ccatgctcga ccatcccgat ttcgcttcct tcgatcttac cagcctgcgc
    10321 accggcatca tggccggttc gccctgcccc atcgaggtga tgaagaaggt ggtgtcgctg
    10381 atgcatatgt cggaggtgac catcgcctat ggcatgaccg aaaccagtcc ggtgtcgttc
    10441 cagagcaatg tcgatgatcc gctggaaaag cgcgtctcca ccgtcggccg cgtccagccc
    10501 catgtcgagg tcaagatcgt cgacgccggc ggcgccacgg ttcgcgtgga cgaagccggc
    10561 gaactgtgca cgcgcggcta ctgcgtgatg aagggctatt ggcaggacga ggaaaagacg
    10621 cgcgaagcca ttgacgagga tggctggatg cattcaggcg acctcgccac cctcgatgcc
    10681 gaaggctact gcaacatcgt cggacgggtg aaggacatgg tcattcgcgg cggcgaaaac
    10741 atttatccgc gcgaggtgga ggaatttctc taccggcatc cgaaaatcaa ggaagtgcag
    10801 gtattcggcg tgcccgacga gaaattcggc gaggcattgg ctgcctggat cgtgctggcg
    10861 cccggcaaca gcgcgacggc ggaagagatc aaggcctact gccagggcca gatatcccac
    10921 tacaagatac cgctctacat ccgcttcaag gaaagcctgc cgatgacggt aaccggcaag
    10981 ccgcagaaat tcgtcatgcg ccaggcgatg atcgacgaac tcggcctcca gccggcacgg
    11041 acggcgtgag gcaacaaaaa aggccccgcg cggggccttt ttcgctggga atcgggactt
    11101 gtcggtcctt tttctcagtc tttttcgaac atattggtgc gggcgaccac gccggcaatc
    11161 gcgccaccga tcaacggagc gacgatgtag agccagagct ggctcagcgc caggccgccg
    11221 gtgaacaaag ccgggccgat cgagcgcgcc ggattaagcg aattgcccga taccggaatg
    11281 aaggcgaagt gcagcgccgc cagcgtcagg ccgatcacca gcccggcaaa agcagtcgta
    11341 tgcttggcgc tggtcacgcc gaggatgacc atgacgaagg tgaacgtcgc gatcacttcg
    11401 gccaggaagg ccgatgaagt gccgtatgcc gccgggtccc agccattcgg ggcaaagccg
    11461 gtggtgtggc cgccgacctg gccggcgacg atgacccaca gggccagcga cgccaggatg
    11521 gcgccgatga tctgcgcgat ccagtagggg atgacgtcct ttgccggcat gcgtccggcc
    11581 aggaatacgc cgagcgttac cgccgggttc aaatgcgcgc ccgaaatagg cccgatggca
    11641 taggccgccg cgataaggcc gatgccgaac gccaggccga tgccgacttg cccgaccggc
    11701 aaagcgccgc caaagccgcc ggttacaacc gaagccgtgc cgatgaacac caatagaaat
    11761 gtaccaagaa cttccgccag ataagtcttc attgccctct cctcgaatag attcgtcgct
    11821 cctgcgaatc accgcgcaac attattcaca agcctgcgcg tttggaagga catgccttcc
    11881 ccgaaacgcg cgccttgtga atgccgtttg ctgtcgttag agcaggagtg aaatcgaaag
    11941 gcggcagcga acaatatgcg actgggtgga cggctggcgg cagccatcga agtgctggaa
    12001 gacatcggac ggcgtcaccg gcctgtggcc gatgccctga aggactgggg cctttcgcac
    12061 cgctttgccg gcggcggcga ccgcgcggcc atcggcaaca tcgtttatga cgcgctgcgc
    12121 cgccggcgct cggcggcatg gcttgtgggc gaggagacgc cgcgcgccat cggcttcggc
    12181 gcgcttctgc tcgacggggg ccgtacagtg gcggcgctga accaggcact ggatggcgac
    12241 aaattcgcgc ccgcgccgct cagcgccgtg gaaatcgctg ccatcgaagc gcggcggctc
    12301 accgatgcgc cggacgaaat ccgcgccgat gttcccggct ggtgcgtgcc gcttttcaag
    12361 gaagcgttcg gcgacgattg ggcggcggaa ggggctgccc ttgcagcgcg cccgccgctc
    12421 gacattcggg tcaacacgtt acgggccaaa cgtgaaagcg tgctggcgga gctggcgcag
    12481 gcgggcgcgg agccgacgct ccttgcgccg catggcatcc gcatcgcccc gatcgacggc
    12541 gatggccgcc accccaacgt gcaggcggag ccggctttcc agaaaggctg gttcgaggtg
    12601 caggacgagg gctcgcagct ggttgccgaa ctatcgggcg ccagggccgg catgcaggtg
    12661 ctggattatt gcgccggggc cggcggcaag acgctggcgc tgtcggcggc gatggataac
    12721 catggccaaa tcttcgcaca cgatgccgag aaggcgcgtc tcgcgccgat cttcgaccgc
    12781 atgcgccgtt cggagaaccg caacgtccag gtggtttcca agccttcgga gcttgctccg
    12841 ctgagcgccc acatggatgt cgtgctggtc gatgcgccct gcaccggcag cggcacctgg
    12901 cggcgcaggc cggatgccaa gtggcggctg acacagcgcc agctcgacgc gcgcaaggcc
    12961 gagcaggcgt ccatcctcga tgccgccatg gcgtatgtga agccgggcgg gctgctcgtc
    13021 tacatcacct gttcggtgtt cgaagaagag aacgccgcgc aaacggccgc cttcctcagc
    13081 cgcgccccag ctttttcgcc ggccgatcat gcggcgctct ggcgccacca cttccccggc
    13141 catgaggctt cggcgcgcat cgacgacgac gccggaatct cgctgtcgcc ggcgcgcagc
    13201 ggcaccgacg gcttttactt ctgcgccatg cgcaaggcag tgtagccagc cacaggtctc
    13261 cccaaccaga gatctccatt gacttcagcc gccgtcgctc cagctagggt cgggcggcgc
    13321 ggatgccgcc ggggctgccg gcaatgaaaa gatccacctc tctcctgttg ttccttgtga
    13381 gcgtggtgag cgccggcctg ctgatcggtt acgccacatt gcccggcgaa tggtatgcgg
    13441 gcctggccaa accgcccttc aatccaccca actggatatt cggcccgacc tggtcgctgc
    13501 tttacgtgct gatcgccatc gcaggctggc gcacatggcg gcgttcccgt tccgatttga
    13561 cgatgaagct ctgtggcgcg cagatgctcc tgaatttcct gtggtcgccg gtcttcttcg
    13621 gcgcgcagca gcccggcccg gctttgattg tcatcgtcgc gctgctggcc gccattgtcg
    13681 gttttatcat cgtgagctgg aaccatgatc gggtatcggc atggcttttc gtgccctacg
    13741 ccgcatgggt ggctttcgcc acgttgctca atgcctcgat atggtggctc aactaatcat
    13801 tttgttgcag cgcagcactg aggcttgtct gcgattgcga cctctgcgat aagccggcta
    13861 tcgcagtggc gaaggcgggt ttcatggcgg caaagatcgt accggctccg gattacgagg
    13921 agcgtgtcag ggcttccttc ctgcgccagc aggcgatggc gacaatcggc gcggaactga
    13981 cgctggtgac gcccggcatc gtcgaaatcg agatggccta ttcggccaag ct
//
LOCUS       DASYCS010000102         5973 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1179212,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000102 DASYCS010000000
VERSION     DASYCS010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 5973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5973
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179212"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..144
                     /locus_tag="VGM46_03645"
     CDS             <1..144
                     /locus_tag="VGM46_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HEY4191716.1"
                     /translation="AKAYLDYAALHRDIIGRFGRFPHRNAALGRETTPEDRAFLDEGG
                     FSG"
     gene            complement(134..652)
                     /gene="folK"
                     /locus_tag="VGM46_03650"
     CDS             complement(134..652)
                     /gene="folK"
                     /locus_tag="VGM46_03650"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015317678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEY4191717.1"
                     /translation="MPGLNNTVYVSLGGNLGDPAKSMGAALRMLDEHEAIRVSAVSSL
                     YRTPPWGKLDQPDFLNAAAGLATTLAARDLLEFCLDVELRLKRVREERWGPRLIDIDI
                     LTYGHRVIREAGLEVPHPRMLERAFVLAPLAEIAPQALIGETSVKQRLAAVDAAGIEK
                     LPSGQDWWRISR"
     gene            complement(636..995)
                     /gene="folB"
                     /locus_tag="VGM46_03655"
     CDS             complement(636..995)
                     /gene="folB"
                     /locus_tag="VGM46_03655"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004688350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEY4191718.1"
                     /translation="MYVIRMKNCAFFARHGVLDEEEALGQRFYVDATLTVDPSRELEC
                     DSIEGTVDYGVAFQVIEAIITGARRFLIESLALEVAKALCGRFPQIRQAEITVRKPNA
                     PVRGVLDYVEVVVAWPQ"
     gene            complement(999..1853)
                     /gene="folP"
                     /locus_tag="VGM46_03660"
     CDS             complement(999..1853)
                     /gene="folP"
                     /locus_tag="VGM46_03660"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEY4191719.1"
                     /translation="MMLRRWRLAHGRHLDLGARAEVMGILNVTPNSFSDGGLFDTLDK
                     AVAQARQMAREGAAIIDVGGESTGPRAVPIDAGEEQARVLPVIEALAEIGETLISIDT
                     YREETARLAVAAGAHIINDVWGLQREPGIARVAAETGAGLIIMHTGRGREKLPDVIAD
                     QFAFLEKSLQIARAAGVADDGILLDPGFGFAKETLDDNLDLMARFQELHALGFPLVAG
                     TSRKRFIGHLTGRDSGAERAAGTAATSVVLRLKGARLFRVHDVAINVDALAVADAMLA
                     RETNPAGN"
     gene            1980..2597
                     /locus_tag="VGM46_03665"
     CDS             1980..2597
                     /locus_tag="VGM46_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018428157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF922 domain-containing protein"
                     /protein_id="HEY4191720.1"
                     /translation="MMKLFLYCVVLFAFAGLPANAANVVRSYSYFSVGGGTLDEIQSQ
                     LSKNGPQVKSTGHRHPGATRMAFTTRLGFARNSTSCKIVSAVVTVKVKVILPRWRRPH
                     NADAGVRLFWDTLSADIKRHEESHIVIARNHAHELEQALLHSASKGNCQTAKAKAKDI
                     SNKILAKHDRAQVQFDRVEGINFESRMLRLLQYRIKRIEDGRLPG"
     gene            2858..4336
                     /locus_tag="VGM46_03670"
     CDS             2858..4336
                     /locus_tag="VGM46_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEY4191721.1"
                     /translation="MDSIANNKPTHFEAPPLSEKEKNVIIAGVLLSMLLAALDQTIVA
                     PAMPTIGGALGHAEYLPWIVTGYLLTATAVAPLYGKISDVYGRRPTIYAAILIFLAGS
                     VVSALAPNMFVLIGGRALQGLGGGGLFALSQTVIGDLVPPRERARYAAWISGTWAVAS
                     IAGPLLGGTFAEHLHWSLIFWINIPLGLLAMIIINNPLKKLPVAARNHGIDGWGALLL
                     VIATSLLLLALNWGGSLYPWVSVEILGILAGSAIVWFLFALRLMHAAEPLISLEVLGN
                     PIVRYGTLSVFLMQGANVGLAVYLPVYMQSSLGLSVSDSGVAMLGLLLGTVSGAMASG
                     NSIPKLTHYKRIALAGALIAIVSLILLSLVAGKESLAIIEILTAGVGIGTGASFPVAT
                     VSVQNAVDRMHLGVATGVLTFLRSLGGALGVAMLGAVALGNGIPLGREGAQAVDDAVS
                     SHAFVMIFLVCTAMVAGAFVTFWLMPEKELRGSVKNTPPALE"
     gene            complement(4375..4695)
                     /locus_tag="VGM46_03675"
     CDS             complement(4375..4695)
                     /locus_tag="VGM46_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008838000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY4191722.1"
                     /translation="MTKLTFIAHDGTHFDVDAENGSSVMENAIRNSIPGIEAECGGAC
                     ACATCHVYVDEAWTAVVGEPAAMEEDMLDFAYEVQPNSRLSCQIRVRDELDGLVVRVP
                     ERQG"
     gene            complement(4802..5170)
                     /locus_tag="VGM46_03680"
     CDS             complement(4802..5170)
                     /locus_tag="VGM46_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015317671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEY4191723.1"
                     /translation="MVGDNGVAFSMPGGEACQPASGRPVDMAHLSRQTMGDRELEQEV
                     LALFLQQALSVSARMRKADVGERLMMAHSLKGSARGVGAFAIADCAAAIENDPQDALA
                     WKRLDGLVEAVRDFVAAIGR"
     gene            5447..>5973
                     /locus_tag="VGM46_03685"
     CDS             5447..>5973
                     /locus_tag="VGM46_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191724.1"
                     /translation="MAKKSTTSKNLDSEVASELEKALDMDLSAETGDLDIAASMEDLE
                     AQISRAADELARAGRSERPAAAAAQAEPAKPAAQKPAAPELRPLESRNGVESRNGAQP
                     SFTPAPGFAPANDDRQKDYRSLLHTLNRRSSTTIYWVVAFVSLAWIAGAGALANLLFG
                     SDVWKIRSLDQLTAQP"
BASE COUNT         1115 a         1909 c         1813 g         1136 t
ORIGIN      
        1 gccaaagcct atctggatta cgctgccctg caccgcgata tcattgggcg cttcgggcgg
       61 tttccgcatc gcaacgcggc gctgggacgt gagacgacgc ccgaggaccg ggcattcctc
      121 gacgaaggcg ggttcagcgg ctgatccgcc accaatcctg gccggacggc agtttttcga
      181 tgccggcggc atctaccgcc gcaagccgct gcttcacgct cgtttcgccg atcaaggcct
      241 gcggcgctat ttcggccagc ggagccagga cgaaggcgcg ctccagcatc cttggatgcg
      301 gcacctcaag cccggcttcg cggatgaccc gatgcccgta agtgagaatg tctatgtcga
      361 tgagacgcgg tccccaacgc tcttcgcgca cccgcttcag ccgaagttcg acgtcaaggc
      421 aaaactccaa caaatcccgc gcggcaagcg tggtggcgag accggctgcg gcattcagga
      481 aatccggctg atcgagtttg ccccagggcg gcgtccggta aagcgaagag accgccgaga
      541 cgcggatcgc ctcgtgctcg tcaagcatgc gcagcgcggc gcccatcgat ttggcgggat
      601 cgcctagatt gccgcccagg ctgacgtaga cggtgttatt gaggccaggc aacgaccacc
      661 tcgacataat cgagcacgcc gcgtaccggt gcgttgggct ttcgaacggt gatttccgcc
      721 tgcctgattt gcgggaagcg cccgcaaaga gccttggcaa cttccagcgc cagagattcg
      781 atcagaaacc ggcgcgcgcc ggtgatgatc gcctcgatca cctgaaaagc cacgccataa
      841 tcgactgtac cctcgatgga atcgcattcc agttcccggc tggggtcgac cgtcagcgtc
      901 gcgtcgacat agaagcgctg gccaagcgcc tcctcctcgt ccagcacgcc gtgccgggca
      961 aaaaaagcgc agttcttcat gcggatgaca tacatcgttc aatttccggc cggatttgtt
     1021 tcgcgcgcca gcatagcatc ggccacggcc agcgcgtcca cgttgattgc gacatcgtga
     1081 acgcgaaaaa ggcgcgcgcc cttcaatctc agcacgacgc tggtggcggc cgttcccgcc
     1141 gcccgctcag cgccgctgtc gcgcccggta aggtggccga tgaaacgctt acgggacgtg
     1201 ccggcaacca gcggaaagcc gagcgcatgc aattcctgga accgcgccat caggtccaga
     1261 ttgtcgtcca gcgtttcctt ggcaaagccg aagcccggat cgagcaggat gccgtcatcc
     1321 gccacgccgg cggcgcgtgc aatttgcagc gacttttcga ggaaggcgaa ctgatcggcg
     1381 atgacgtcgg gcagtttctc gcgcccgcgc ccggtatgca tgataatcag ccccgcgccg
     1441 gtttcggccg ccaccctggc gatgcccggc tcgcgttgca ggccccatac atcgttgatg
     1501 atatgcgctc cggcggcaac cgccagccgc gccgtttcct cgcgataggt gtcgatggaa
     1561 atcagcgttt cgccgatttc ggccagagcc tcgatgaccg gcagcacgcg cgcctgttct
     1621 tccccggcgt cgatgggcac ggcacgcggg ccggtcgatt caccgccgac atcgatgatg
     1681 gcggcccctt cgcgtgccat ctggcgcgcc tgcgccaccg ccttgtccaa cgtatcgaac
     1741 aggccgccgt cggaaaagga gttgggcgta acattgagga tgcccatcac ctccgccctg
     1801 gcgccaaggt ccaggtggcg tccatgcgcc aatcgccatc ttctcaacat catcgtcatt
     1861 accggtaacc cagcccggcc gcgcgaccgc gcttcttgcg ttcgacgcgg caggcactat
     1921 tcaggacaaa ttcggttgcg ccgaaaatgg cggtaaagca aggtggcgac agaggcaaca
     1981 tgatgaagct gttcctgtat tgcgtcgtgc tttttgcatt tgccggcctg ccggcgaatg
     2041 cggccaatgt ggtcaggagc tatagctatt tcagcgtcgg cggcgggact ttggatgaaa
     2101 tccagagcca attgtcgaag aacggccccc aggtgaaaag caccggccac cgccaccccg
     2161 gcgcgacgcg gatggctttc accacgcgcc tgggctttgc ccgcaactcc acgtcctgca
     2221 agatcgtttc cgctgtcgtg accgtgaagg tgaaagtcat tcttccccgc tggagaagac
     2281 cccacaatgc ggatgcgggc gtcaggctgt tctgggacac gctgtcagcc gacatcaagc
     2341 ggcatgagga aagccatatc gtcatcgcca ggaaccatgc ccacgaactg gagcaggcgc
     2401 tgctgcattc cgcgagcaag ggcaattgcc agactgccaa ggcgaaggcc aaggacatct
     2461 ccaacaaaat attggccaag cacgaccgcg cgcaggtgca gttcgaccgc gtcgaaggca
     2521 tcaatttcga aagccgcatg ctccggctgc tccaataccg catcaagcgg atagaagatg
     2581 ggcgcttgcc gggctaacga tttggccgat aacgatttcg gcactcgtct gccttcgcct
     2641 cgacaccctc atgcaggtct gatattgccc ccgcgctgtt ttaaggaaac gcgtgccggt
     2701 tggtagtccg gcggcctgtc aggtccgaag cccgtcttgc gacgtggtcc acagccaaca
     2761 ccggggggaa accggaccgc agccgcgcct ttcagcccgg cggcatcggc cgataccttt
     2821 ggaaatggca atgcgtgcag gcgcaatgag tggatcgatg gacagcatag caaacaacaa
     2881 gcccacacat ttcgaagcgc cgcctttgtc cgaaaaggaa aagaacgtca tcatcgccgg
     2941 cgttttgctg tcgatgctgc tggcggcgct cgaccagacc atcgttgcac cagcaatgcc
     3001 gaccatcggc ggagcgctcg gccatgccga atacctgccc tggatcgtta ccggctatct
     3061 tttgacggca acggccgtgg cgccgcttta tggcaagatt tccgatgtct atggacgtcg
     3121 cccgaccatt tatgcggcga tcctcatttt cctggccgga tcggtcgtca gcgcgctggc
     3181 gccaaacatg tttgtgctga tcggcgggcg ggccttgcag ggtctgggtg gcggcggtct
     3241 tttcgcgctg agccagacgg tgatcggcga tctcgtgccg ccgcgcgagc gcgcccgtta
     3301 cgccgcctgg atttccggca cctgggccgt cgccagcata gccggcccgc tgctgggcgg
     3361 cacatttgcc gagcatctgc actggtcgct gatcttctgg atcaacattc cgcttggtct
     3421 gctggcgatg ataatcatca acaacccgct caagaaactg ccggtcgccg ccaggaacca
     3481 cggcatcgat ggctggggcg cgctgctgct cgtcattgca acctcgctgc ttctgctggc
     3541 gctgaactgg ggcggcagcc tctatccatg ggtttcggtg gaaatccttg gcattcttgc
     3601 cggctcggcc atcgtctggt ttttgtttgc gctgcgtctg atgcatgcgg cggagccgct
     3661 gatatcgctg gaggtgctcg gcaatccgat tgtccgctac ggcacgcttt cggtgttcct
     3721 gatgcagggc gccaatgtcg ggctggccgt ctacctgccg gtctatatgc aatcctcgct
     3781 cggcctgtcg gtgagcgatt ccggcgttgc catgcttggc ctccttttgg gcaccgtctc
     3841 gggcgcgatg gcgagcggca attccatccc gaagctgacc cactacaagc gcatcgcgct
     3901 ggccggcgcg ctgatcgcca ttgtcagcct catcctgctc agcctggtgg ccggcaagga
     3961 atcgctggcg ataatcgaaa tcctgaccgc gggcgtcggc ataggcacgg gcgctagctt
     4021 ccccgtcgca acggtgtcgg tgcaaaacgc cgtcgaccgc atgcatctcg gcgtggcgac
     4081 aggcgtgctc acgttcctgc ggtcgctggg cggcgcgctt ggcgtcgcca tgctcggcgc
     4141 ggtggcgctc ggcaacggca tcccgcttgg gcgcgaaggc gcgcaggcgg ttgacgacgc
     4201 cgtatcttca cacgctttcg tgatgatatt cctggtctgc accgccatgg tggcgggagc
     4261 cttcgtcacc ttctggctga tgccggaaaa ggaactgcgc ggctccgtca agaatacgcc
     4321 gccggcgctg gaataggcgc agccatccga accggaattg gtagcggcag gcgtttagcc
     4381 ctgccgctcc ggcacgcgca ccaccagccc gtccagttca tcgcggaccc tgatctggca
     4441 ggaaaggcgc gaattgggct gcacctcgta ggcgaagtcg agcatgtctt cttccatggc
     4501 cgccggctcg ccgacgaccg ccgtccaggc ctcatcgaca tagacatggc aggtagcgca
     4561 ggcgcaggcg ccgccgcatt ctgcctcgat gccggggatg gagttgcgaa tggcgttttc
     4621 catcaccgat gagccgtttt cggcatctac gtcgaaatgg gtgccgtcgt gggcgataaa
     4681 ggtcagtttg gtcatttgtc acctgaagga gtctgcgccg agataatcac tcgcgcgcac
     4741 aagtcaacca cgcgcgaaaa cgccgcaccg tgacgttttt caaaaactgg cggtgcagcc
     4801 cttagcggcc gatggccgcg acgaaatcgc ggacggcttc gacgaggccg tcgaggcgct
     4861 tccacgcaag ggcatcttgc ggatcgttct cgattgccgc cgcgcaatcg gcaatggcga
     4921 atgcgcctac cccgcgcgcg gaacctttca aactgtgcgc catcatgagg cgctcgccga
     4981 catcggcctt cctcatcctg gcgctgacgc taagagcctg ctggagaaac aacgccagca
     5041 cctcctgttc aagctcgcgg tcgcccatcg tttggcgcga aagatgcgcc atgtcgaccg
     5101 gcctgccgga agccggctgg caggcttcac cgcccggcat ggaaaaggct acgccattgt
     5161 caccaaccat cgcagaccgt ctcccgtcgc cacacaaatg gctgtttgca ggccagaatg
     5221 ctatggccgg cgggttaacg gggcgttgca aaaaaccgcc gatcgcgagc ttctaaatca
     5281 cggcgacgtt aaccttatat ttaaagtttt acgtattcgg ccgaatgcat gttttcttta
     5341 cccccctgtg tcattacgat acgaggggtc catattgagc ctcaggcgcg gggttaataa
     5401 gcgataaaaa caggttccgc ggcagctagt acagggtagc gaaggcatgg cgaagaaatc
     5461 gacaacctca aagaatctcg acagcgaagt ggccagcgag cttgaaaaag cgctggacat
     5521 ggatttgtcc gcggaaaccg gcgatctcga tatcgccgcc tccatggagg atctggaagc
     5581 gcagatttcg cgggcagcgg acgaactggc gcgcgccggc cgctccgagc ggccagcggc
     5641 tgcggctgct caggccgagc cggcaaagcc ggccgcccag aagccggcag cgcccgaact
     5701 gcgtccgctc gagtcccgaa atggggtcga gtcccgaaat ggggcgcaac cttcctttac
     5761 gcccgctccc ggtttcgcgc cggccaatga cgaccggcag aaagactacc gctcgctgct
     5821 tcacacccta aaccgccgtt cgtcgaccac catctattgg gtcgtcgctt tcgtttcgct
     5881 ggcctggatt gccggcgccg gtgcgctcgc caatctgctg ttcggcagcg atgtctggaa
     5941 aatccgttcc ctcgaccagt tgacggcgca gcc
//
LOCUS       DASYCS010000103         7750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4804478,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000103 DASYCS010000000
VERSION     DASYCS010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 7750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7750
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4804478"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="VGM46_03690"
     CDS             <1..486
                     /locus_tag="VGM46_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEY4191725.1"
                     /translation="YGADSTTGAPDATNFAKAGIIDGARTKDGAKASALGTTVVLGAA
                     SAAGDTYNVASLALVSGGKTASDSQISDMIGVVEGALKDMTDAATTLGANKSRIDLQK
                     TFTQSLMDSIDRGVGQLVDADMNKESTRLQALQVQQQLGIQALSIANGSSQSILSLFR
                     G"
     gene            727..1791
                     /locus_tag="VGM46_03695"
     CDS             727..1791
                     /locus_tag="VGM46_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEY4191726.1"
                     /translation="MASIMTNASALTALQSLNATNKSLEQTQARISTGYRVADAADNA
                     AYWSIATTMRSDNQALSTVQDALGLGASKVDTAYTGMNNAITVADQIKVKLVAAFNAS
                     DSDKAKIQTEIKSLQAQVKSYADAATFSGANFLSVTSKQVAAANDGVQQDAQIVSSFN
                     RSSSGAVTLGTIDIDVEGTKLFDSGLATEVKNQGLLDRATSIYSTAADQKLYDDAYSA
                     QIALGKSDKDAHAAAILVATSTDKVDNVSLYNIDITAPGVTNDIINQMITKADNVLNQ
                     LTDAATVLGSAKKQIDLQKTFTQSLMDSIDRGVGQLVDADMNKESTRLQALQVQQQLG
                     IQALSIANSNSQNILALFKS"
     gene            1921..3564
                     /gene="fliF"
                     /locus_tag="VGM46_03700"
     CDS             1921..3564
                     /gene="fliF"
                     /locus_tag="VGM46_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006204080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HEY4191727.1"
                     /translation="MPEQIQTIISNLKGFGAKRLALLAGIAALVIAVIGIGSVYLNRP
                     AYETLYVGLERSDVNQIGMVLGEAGIGFDVGSDGTTVLVSAGTTAQARMLLAEKGLPT
                     SAKAGYELFDNVGSLGLTSFMQQITRVRALEGEIARTIQSISGIKAARVHIVMAERAN
                     FRREEQQPSASVVIRYSGADGEKTAMSIRHLVAAAVPALSADRVTVLDSSGNLLASGD
                     DPSNTSATRALGIEQSVEAQIAENIRRALTPYLGPDNFRASVKADVNTDTRQTEETTY
                     DPESRVERSVQSMRNNENNSQKQTSTPASVEQNLPEAQTATSEGPQSTSQNDRKEEIT
                     NYEINSKKIATVSNGYSVEKMSIAVVVNRQRLMTILGKDASAEQIAARVAEIQKMVAS
                     ATGFSDKRGDVIDVSSVEFIDGLDGEPIEQAGIMESIGHYTGSLINAGAFIVVVFLVA
                     FFGLRPMAAALTAQAKPALADPSFDEVQRALPTPEATADGGQAATTALPGRGGSNALD
                     ELRQKLRPAPQERLARMVDLNEERTAQILRKWAAQEAVG"
     gene            3564..4184
                     /locus_tag="VGM46_03705"
     CDS             3564..4184
                     /locus_tag="VGM46_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191728.1"
                     /translation="MRSAALFDLLPDFGASLPRAVPVDAAREHAARSDNPPVDIEAIV
                     ARAVADAEAALEERLTKAHEEALEAERQANAQEAKLFLESLGDDVGKTIAARIDAAQS
                     HLGELAGTAVTRIVGGLLSEDLQKRSVTALARTIRDSIGDPEAVRIAVRGPQSLFETL
                     SAALGAYADHLDFTEASSLDLTVVIDDAVFETRMAEWGEALTEVLL"
     gene            4181..5458
                     /locus_tag="VGM46_03710"
     CDS             4181..5458
                     /locus_tag="VGM46_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotB family protein"
                     /protein_id="HEY4191729.1"
                     /translation="MSTAEAHEAPHEIIIVRHGHGDHDEGHHGGVWKIAFADFMTAMM
                     CFFLVMWLINAANEQTKAAVASYFNPVKLIDRNASRKGLEDLGDGPRASGLTADSPQT
                     DADKAGQGGDGGSVSSQSKESEAAPKREDNADAHLFADPYAVLSEIATDTGTMQNVSQ
                     KGDGGAQKAGPATGASGGVSYRDPFAPDFWSQQMADPAAEASAERARIEGDPAKPGDK
                     TAETENPKVKAVPPKPASDFAPLEPLAKEAGEAPSVEEVAKPAGAKAEKTPSPAAQKA
                     AARVKQQLAEAFKDRDKLHDGISVKATDKGVVISITDQLDFGMFKIGSAMPDRELVLA
                     MDKIGRIVNGQKGKISIGGHTDARPFRSETYDNWRLSAARAQSAYYMLVRGGVDEHRI
                     VEVSGFADRQPKDAADPLAAANRRIEILLETDG"
     gene            5455..6876
                     /locus_tag="VGM46_03715"
     CDS             5455..6876
                     /locus_tag="VGM46_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein MotC"
                     /protein_id="HEY4191730.1"
                     /translation="MKGATLISGLIGPALVSGLIGISLLALPRLAYAADALQPFQLVR
                     SLQLVQDRIAAGDHAALPMQRKLLEMVDARMRTAAPDAFKDPKNFRALLVYGMSGGNP
                     VTVEAATARLDLDAENHALADGVIAYLSGRPAVAIAALEPIEPMHLPSDLGAFLALVK
                     GSLLAADKPVEALALLDQARLLSPGSLVEEAALRRSVAIDAAQGDAARFALASTQYVE
                     NYLYSPYASQFADAFVNGVIAMHQAISQDKLADITSMMDAEREKVIYLRIARRAAIEG
                     LNDLSTYASAKAEQGRDGSGNENDPRAQLYASLSTVTSATVGDLREKLKTIDRSQLSE
                     RDQSLLDAAQAVIGEMVAVPKAPSPEHQATVARPATPTAGAPEAAEQASVASDLPAVE
                     GAMPEQPAVDKPPVDTPVAPSPVPVSATETAPVQEISMSAKAAPLPPGKTATAAPDAA
                     DTAIADAHRRLESIDQMLGTSPK"
     gene            6879..>7750
                     /locus_tag="VGM46_03720"
     CDS             6879..>7750
                     /locus_tag="VGM46_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191731.1"
                     /translation="MSTGPALTDQLPAGTHAAAKAARGKTDSDFHHILYQADGKKHAT
                     GPQHAQDQAADDPVRQRLWPEFIGKFADSDGDPEKITADDADKSSDAGKKIDDGKVRH
                     GDRHHDQMHDPQIHDPSQAMAALPGAGLEPARGHADESQAAPAAPTQNPADVAQPQPI
                     LGETEVDLNLSGKVMPTNAPLQPTKSHASETNNIAGQQDDGFAGLPADADEAGSPEPL
                     VAVKAKPGSDGQTARQPSADKPASPAHVTVIGEQNFPAPAAYQSSTATSLAATIGGDG
                     GLRQSLAASAGLAST"
BASE COUNT         1586 a         2635 c         2290 g         1239 t
ORIGIN      
        1 tatggcgccg acagcacgac cggtgctccc gatgcgacca acttcgccaa ggccggcatc
       61 atcgatggcg cccgcaccaa ggacggcgcc aaggcgtcgg cgcttggaac gaccgtcgtt
      121 ctcggcgctg cttccgctgc cggtgacacc tacaacgtcg cctcgcttgc tctcgtctcg
      181 ggcggcaaga ccgccagcga cagccagatc tccgacatga tcggcgttgt cgaaggcgcg
      241 ctgaaggaca tgacggatgc cgccacgacg ctcggcgcca acaagagccg catcgacctg
      301 caaaagacct tcacgcagag cctgatggac tccatcgacc gtggcgtcgg ccagctcgtc
      361 gatgccgaca tgaacaagga atccacccgc ctccaggcgc tccaggttca gcagcagctc
      421 ggcatccagg cgctgtccat cgccaacggc tcctcgcagt ccatcctgtc gctgttccgc
      481 ggctaagcct ttccacccat ccactgacga aaaaagggcc gcgcttcatg cgcggccctt
      541 tttgttttcg ccggaccagg ccttcatgcc ttcgaaagaa cctttaatcg gccattaacc
      601 atatcgcgct agtcatggtt aaccaatagc ttacgacact ggcgaatcgg catctgccaa
      661 gccagccgtc taacgggccg atcgggcgtg tggaagcacg ccagcttttg aaaaggggtg
      721 agtaccttgg cgagcattat gacgaacgct tcggcgttga ccgcacttca aagccttaac
      781 gccacgaaca agtcgcttga gcagacgcag gcgcggattt cgaccggcta ccgggtcgcc
      841 gacgcggccg ataatgccgc ctattggtcc atcgccacga cgatgcgctc cgacaaccag
      901 gcgctttcga cggtgcagga tgcgctgggc ctcggagcct cgaaggtcga caccgcctat
      961 acgggcatga acaatgccat caccgtcgcc gaccagatca aggtcaagct cgtcgccgcg
     1021 ttcaacgcca gcgattcgga caaggccaag attcagaccg agatcaagtc gctccaggcg
     1081 caggtgaagt cctacgccga tgccgccact ttctcgggcg cgaatttcct gtcggtgacc
     1141 tccaagcagg ttgccgccgc caatgacggc gtccagcagg acgcccagat cgtctcttcc
     1201 ttcaaccgct cctcgtcggg cgcggtcacg ctcggcacca tcgacatcga cgtcgagggc
     1261 accaagctgt tcgattcggg tctcgccacc gaagtcaaga accagggcct gctggatcgc
     1321 gcaacgtcca tctattccac ggctgcggac cagaaactct acgacgacgc ctattccgcc
     1381 cagatcgccc tcggcaagtc ggataaggac gcccacgccg ccgcgatcct cgtcgccacg
     1441 agcaccgaca aggtcgacaa tgtctctctc tacaacatag acatcaccgc gcccggcgtt
     1501 accaacgaca tcatcaacca gatgatcacc aaggccgaca acgtgctgaa ccagctgacc
     1561 gacgcagcca ccgtgctcgg ctcggccaag aagcagatcg acctgcaaaa gactttcacg
     1621 caaagcctga tggactccat cgaccgcggc gtcggccagc tcgtcgatgc cgacatgaac
     1681 aaggaatcca cccgcctcca ggcgctccag gttcagcagc agctcggcat ccaggcgctg
     1741 tcgatcgcca actcgaactc gcagaacatc ctggcgctgt tcaagagcta acacgcagcc
     1801 gcgccgtaat taatctccct tgaaaaatcg ggccgcgctg caagcgtggc ccgatttcat
     1861 tttcgcacaa gcttcgcagt ctagtgtcgt catgggcaat gatgcgggag cgttcgatcc
     1921 gtgccggaac agatccagac aatcatctcg aacctcaagg gtttcggcgc caaacgtctt
     1981 gcccttttgg ccggcatcgc cgccctggtc atcgccgtga tcggcatcgg ctcggtctat
     2041 ttgaaccgtc ccgcctatga aacgctctat gtcggcctcg aacgctcgga cgtgaaccag
     2101 atcggcatgg ttcttggcga agccggcatc ggcttcgatg tcggctcgga tggaacgacc
     2161 gtgctggtgt cggccggcac taccgcgcaa gcgcgcatgc tgcttgctga aaaaggcctg
     2221 ccgaccagcg caaaggccgg ctacgaattg ttcgacaatg tcggctcgct cggcctgacc
     2281 tccttcatgc agcagatcac ccgcgtgcgg gcgctggagg gcgaaatcgc ccgcaccatc
     2341 cagtccatat ccggcatcaa ggcggcgcgg gtccacatcg tgatggccga acgcgccaat
     2401 ttccggcgcg aggaacagca gccttcggcg tcggtggtca tccgctacag cggcgccgat
     2461 ggcgaaaaga ccgccatgtc catccgccac ctggtggccg ccgccgtgcc cgctctgtcg
     2521 gcggaccggg tcacggtgct tgattccagc ggcaacctgt tggcttccgg cgacgatccc
     2581 tccaacacca gcgcgacccg cgcattgggc atcgagcagt cagtcgaggc gcagatcgct
     2641 gaaaacattc gccgcgcgct taccccttat ctcggcccgg ataatttccg cgccagcgtc
     2701 aaggccgacg tcaataccga cacccgccag acggaagaga cgacctacga tcccgaatcg
     2761 cgcgtcgagc gctcagtgca atcgatgcgc aacaacgaaa ataacagcca gaagcagact
     2821 tccacgccgg caagcgtcga acagaacctg cctgaggccc agacggccac cagcgaaggt
     2881 ccccagtcca cctcgcagaa cgaccgcaag gaagaaatca ccaactacga gataaattcc
     2941 aagaagatcg ccactgtttc caacggttat tcggtggaaa aaatgtcgat cgcggtggtg
     3001 gtcaaccggc agcgcctgat gacgatcctc ggcaaggacg cctcggcgga gcagattgcg
     3061 gcgcgcgtcg cggaaatcca gaaaatggtg gcctccgcca ccggcttcag cgacaagcgc
     3121 ggcgatgtga tcgacgtttc gtcggtggaa ttcatcgacg gtctcgacgg cgagcctatc
     3181 gaacaggccg gcatcatgga atcgatcggc cactacaccg gcagcctgat caacgccggc
     3241 gccttcattg tggtggtttt cctcgtcgcc ttctttggcc ttcgcccgat ggcggcggcc
     3301 ctgaccgccc aggcaaagcc tgctttggcc gatccaagct tcgacgaagt gcagcgcgcg
     3361 ctgccgacgc cggaagcaac cgccgatggc ggccaggcgg caactaccgc cctgcccggt
     3421 cgcggcggtt cgaacgcgct ggacgaattg cgtcagaaac tgcggcccgc gccgcaggag
     3481 cgtctagccc gcatggtcga cctgaacgag gagcgcaccg cccaaatcct gcgtaaatgg
     3541 gcggcccagg aagccgtggg ctgatgcgtt ccgcagcttt attcgatctt ttgccggatt
     3601 tcggcgcgag cctgcctcgc gccgttcctg tcgatgcggc gcgcgaacat gcggcccggt
     3661 ccgacaatcc cccggtcgat atcgaggcca tcgtggcgcg tgccgtcgcc gatgccgaag
     3721 ctgcattgga agagcggctg accaaggcgc atgaagaagc gctggaagcc gagcgccagg
     3781 ccaatgcgca agaggccaag ctgtttctcg aaagcctcgg cgacgatgtc ggcaagacca
     3841 tcgccgcccg catcgatgct gcgcagtcgc atctcggtga actggccggc acggcggtca
     3901 cccgcatcgt cggcggcctg ctcagcgaag acttgcaaaa acgttccgtc accgcgcttg
     3961 cgcgcaccat tcgcgacagc attggcgacc ctgaggccgt gcgcattgcc gtgcgcggtc
     4021 cgcaatcgct gttcgaaacc ttgagcgctg cactgggggc ctatgccgac cacctcgatt
     4081 tcaccgaggc gtcgagcctg gacctcaccg tcgtcatcga cgacgccgta tttgaaaccc
     4141 gcatggcaga atggggcgaa gcgctgaccg aggtgctgct atgagcaccg ccgaggcgca
     4201 cgaagcgccg cacgagatca tcatcgtgcg ccatggccac ggcgatcatg acgagggcca
     4261 ccatggcggc gtctggaaga tcgcatttgc cgatttcatg accgccatga tgtgcttctt
     4321 cctggtcatg tggctgatca atgccgccaa cgagcagacc aaggcggccg tcgccagcta
     4381 tttcaatccc gtcaagctga tcgaccgcaa tgccagccga aaagggctgg aagatctcgg
     4441 cgacgggcca cgtgcctccg gcctgacggc cgatagcccg caaacagacg ccgacaaagc
     4501 cggccagggc ggcgatggcg gttcggtttc ctcgcaatcg aaggaatcgg aagccgcgcc
     4561 caaaagagaa gacaatgcgg acgcgcatct tttcgccgac ccctatgccg ttctctcaga
     4621 gatcgcgacc gacaccggca cgatgcagaa cgtcagccaa aagggcgacg gcggcgcgca
     4681 aaaggccggc ccggccaccg gcgcttccgg cggcgtatcc taccgcgatc ccttcgcgcc
     4741 cgatttctgg tcgcagcaga tggccgaccc cgccgccgag gccagcgccg agcgcgccag
     4801 gatcgagggc gatccggcca aaccgggcga caagaccgcc gagactgaaa atccgaaggt
     4861 aaaggccgta ccgccgaaac cggcgtcgga cttcgcaccg ctcgaaccgc tggccaagga
     4921 ggccggcgaa gcgccgtccg ttgaggaagt ggcgaaaccg gccggcgcaa aggcggaaaa
     4981 aacgccttct cccgcagccc agaaggcggc ggcgcgggtg aagcagcaac ttgccgaggc
     5041 cttcaaggac cgcgacaagc ttcatgacgg catatcggtc aaggcgaccg acaagggcgt
     5101 cgtcatctcc attaccgatc agctggattt cggcatgttc aagatcggct cggcgatgcc
     5161 cgaccgtgaa ttggtgctgg cgatggacaa gatcggccgg atcgtcaacg gccagaaggg
     5221 gaagatctcc atcgggggcc acaccgacgc ccgcccgttc agaagcgaga cgtatgataa
     5281 ttggcggctg tcggcggccc gcgcgcaatc ggcttactac atgctggtgc gcggcggcgt
     5341 cgacgaacac cggatcgtcg aagtatccgg ctttgcggac cgccagccga aggacgcggc
     5401 cgatccgctc gccgccgcca accggcgcat cgaaatcctg ctggaaaccg acggatgaaa
     5461 ggcgcaacgc tgataagcgg cctgatcggc ccggcgctcg taagcggctt gatcggcatc
     5521 tcgcttctgg ccctgccgcg cctcgcttat gccgcagacg cgctccagcc attccagctt
     5581 gtccgctccc tgcaactcgt ccaggaccgc atcgccgccg gcgatcatgc ggccctgccg
     5641 atgcagcgca aattgctgga aatggtcgat gcgcgcatgc gcaccgccgc gccggacgcc
     5701 ttcaaggacc ccaagaattt ccgcgcgctg cttgtctacg gcatgagcgg cggcaatccg
     5761 gtgaccgtcg aggccgccac cgcccgcctc gatctcgatg ctgaaaatca cgccttggcc
     5821 gatggcgtca tcgcctatct gagcgggcgt ccggccgtgg cgatcgccgc cctcgaaccg
     5881 atcgaaccga tgcatttgcc gagcgatctt ggcgcgttcc tggcgctcgt caagggttcg
     5941 ctcctggccg ccgacaagcc tgtcgaggcg ctcgccctgc tcgatcaggc gcgcctgctt
     6001 agcccaggct ccttggtcga ggaagcggcc cttcgccgtt cggtcgccat cgacgccgcg
     6061 cagggcgacg ctgcccggtt cgcgctggca tccacgcaat atgtcgagaa ctatctatac
     6121 tcgccctatg ccagccagtt tgcggacgcc ttcgtcaatg gcgtgatagc catgcatcag
     6181 gcgatcagcc aggacaagct cgccgacatc acctcgatga tggatgccga gcgcgaaaag
     6241 gtgatctacc tgcgcatcgc gcgccgcgcc gcaatcgagg gtttgaacga cctttcgacc
     6301 tatgcgtcgg ccaaggccga gcaggggcgc gacggcagcg gcaacgaaaa cgacccgcgc
     6361 gcgcagcttt atgccagcct ttcgaccgtc acctcggcca ccgtcggcga cctgcgcgaa
     6421 aagttgaaga ccatcgaccg cagccagctt tcagagcgcg accagtcgtt gctcgacgcc
     6481 gcccaggccg tgatcggtga aatggtcgcg gtgcccaagg cgccctcgcc ggaacatcaa
     6541 gccaccgtcg ccagaccggc aacgccgaca gccggcgccc cggaagccgc cgaacaggct
     6601 tccgtcgcgt cggatctgcc ggcggtcgag ggcgcaatgc cggagcagcc ggcggtggat
     6661 aagccgccgg tcgatacgcc ggttgcacct tcccctgttc cggtttctgc aaccgagacc
     6721 gcgccggtgc aggaaatttc catgtcggct aaggccgcgc ccttaccccc aggcaaaacg
     6781 gcaactgccg cgcccgacgc cgccgacacc gccatcgccg acgcacaccg ccgacttgaa
     6841 tccatagacc agatgcttgg gacatcgcca aaatgagagt gagcaccggc cccgccctga
     6901 ccgaccagct tcccgccggc acccacgccg cagccaaggc cgcgcgtgga aagaccgaca
     6961 gcgattttca ccatatcctg tatcaggcag atggaaaaaa gcatgccacc gggccacaac
     7021 acgctcaaga ccaggcggct gacgatccgg ttcgccagcg gctttggccc gaattcatcg
     7081 gcaagttcgc cgatagcgac ggcgatcccg aaaaaatcac ggccgacgat gccgataaat
     7141 catccgatgc cggcaagaag atcgatgacg gcaaggtccg gcatggcgac cggcatcatg
     7201 accagatgca cgatccccag atacacgacc cgtcgcaggc catggcggct ttgccgggcg
     7261 caggcctcga accagcacgt ggccatgcag atgaaagcca agcagcgcct gccgcgccaa
     7321 cgcagaatcc cgccgatgtc gcccagccac agccgatttt gggcgaaact gaagtggatt
     7381 tgaacctttc gggcaaggtt atgccgacaa acgcgccctt gcagcccaca aaaagccatg
     7441 caagcgagac aaacaacatc gccggccagc aggacgatgg gtttgcgggc ctgccggccg
     7501 acgccgacga ggcgggatca ccagagcctc tggtggcggt caaggcgaaa cccggaagcg
     7561 atgggcagac tgcgcggcaa cccagcgccg acaagccggc ttcaccggcg cacgtcaccg
     7621 tcatcggcga gcaaaatttt ccagcgccgg cggcctatca gtcatccacc gccaccagtc
     7681 tcgccgccac tatcggtggc gatggcggcc ttcgccaatc ccttgcggca tccgccgggc
     7741 ttgcctcgac
//
LOCUS       DASYCS010000104         3281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3935023,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000104 DASYCS010000000
VERSION     DASYCS010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3935023"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..417
                     /locus_tag="VGM46_03725"
     CDS             178..417
                     /locus_tag="VGM46_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006333070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HEY4191732.1"
                     /translation="MAKLVYKVVEHDGGWAYKVGSTFSETFPSHAQALHAAERASAEQ
                     QVSGTTDGIVYEDADGNWHEELADGHDRPQTEVSD"
     gene            648..878
                     /locus_tag="VGM46_03730"
     CDS             648..878
                     /locus_tag="VGM46_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191733.1"
                     /translation="MAAATIGWNDLTKDEQTALKRMNRGPYPDLEPAMAQRLIALGLA
                     QDRPRGIGISRTGRDLVITTLLEARENLPPAG"
     gene            1044..2324
                     /locus_tag="VGM46_03735"
     CDS             1044..2324
                     /locus_tag="VGM46_03735"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006199908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY4191734.1"
                     /translation="MAAIPLIEETNGGSAGAMVSGLAGFAPDSTGAAHVELANNRPER
                     KLAIYPASAGFDLVEELDYLCSRTVEPNVFFNPRFLAPAMPRLEDREVRLAVIRDGDD
                     HRNRLRLLVPFSVERSMLPMGASIMRTWASPFGPIGTPLIDRDDPVGVIEDFFSMLAR
                     PHLKLPKVLVLPDMRLDGPTASLLRMATEARSLPLVTTGEVQRPVLQSELDGDDYLKN
                     ALRPHHYREFRRLKRRLGERGHLEHIVARGPEQIRHAIESFLTLEAAGWKGRERTAMA
                     IDRYRAAFAREAVHRLAEQDLCRIHSLVLDGRTIASLIVFVEQGFAYTWKTAYDETLS
                     AFSPGALLMIEATRQHLDDPNITMTDSCTVPDHPVMSRLWTERRPVGTIVIGLTPDSD
                     RLARQAAAQLHLHRETRNMARIVRNRMKSLLSRR"
     gene            2454..>3281
                     /locus_tag="VGM46_03740"
     CDS             2454..>3281
                     /locus_tag="VGM46_03740"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006332122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEY4191735.1"
                     /translation="MSAQKTRTETDTFGPIEVASDRYWGAQAQRSLGNFKIGWEKQPA
                     SIVRALGIVKRAAAEANMDLERLDPALGKTIVQAAQEVIDGKLNEHFPLVVWQTGSGT
                     QSNMNANEVISNRAIELLGGVMGSKKPVHPNDHVNMSQSSNDTYPTAMHIASAERIVH
                     DLLPALKHLHAALDAKAKAFDHIVKIGRTHTQDATPLTLGQEFSGYAAQVASSIKRIE
                     LTLPGLCELAQGGTAVGTGLNAPIGFAEKVADRIAAITGIDFKTAPNKFEALAAHDSM
                     "
BASE COUNT          678 a         1079 c          995 g          529 t
ORIGIN      
        1 cacgatgcag cattggatga aagagacgct gtaagccgca agcaaatacc tgttggcgat
       61 ggctgcgagg cgcacaggcg acaccgcaac cattgaccgc gccttgcgtt cctgaaagcc
      121 ttgccggcgg gtggacctga cggtcaggcc cgcccaaaac caaacccgga gattccaatg
      181 gcgaaacttg tctacaaggt agtcgaacac gatggcggct gggcctacaa ggtcggatcg
      241 accttttccg aaaccttccc cagccacgca caggccttgc acgccgccga aagggcgtcg
      301 gcggaacagc aggtctcggg gacgacggac gggatcgtct atgaggatgc cgacggcaac
      361 tggcatgagg aattggccga cggccacgat cggccacaga cggaagtctc cgactgatgc
      421 cgcgcatcga gaccgaatga cctaaagaca agccaataca ataagataag cgcggacagg
      481 cgaaatccat ggcgtgtccg ctgcgattgt ccgatgcgca ggatacgaca ttgcctgccg
      541 ccaaccatgc tttgtcgagg ctggctttcg tggtctgtaa agccaggacg ccggattgat
      601 ccgcccatgg taggcaaagc ccggctcctt gggcaggaca tacgggaatg gcggcagcga
      661 caatcggctg gaacgacctg acgaaagacg aacagaccgc attgaagcgc atgaatcgcg
      721 gtccgtatcc ggacctcgaa ccggcaatgg cccagaggtt gatcgcgctg ggactggcgc
      781 aggacaggcc gcgcggcatc ggtatcagcc gcaccgggcg cgacctggtg atcaccactt
      841 tgctcgaagc gcgcgagaac cttccgcccg ccggttgaac aagtggcttc gacgcctcgc
      901 aagccgggcg ttccggcgaa agcaaaccgc ttggccaacc gtttcttaag gtgtctttgc
      961 tagatagcgg ggcttgaaca gggccgaata cgggcagttc aggtgcggtt ttcggcggcc
     1021 acgcctgcca agcgaggaat atgatggccg ccatccccct gatagaagaa acgaatggcg
     1081 gctccgccgg cgcaatggtt tcgggccttg ccggcttcgc gcccgacagc accggcgccg
     1141 cccatgtcga actggccaat aaccggccgg agcgcaagct ggccatctac ccggcatcgg
     1201 cgggcttcga cctggtcgag gaactggatt atctttgctc ccgcacggtc gagccgaacg
     1261 tcttcttcaa cccgcgcttc ctggccccgg cgatgccgag gctggaagac cgcgaggtgc
     1321 ggctggccgt cattcgcgac ggcgacgacc atcgcaaccg gctgcggctt ttggtgccgt
     1381 tttcggtgga aagatcgatg ctgccgatgg gcgcgtcgat catgcgcacc tgggcaagcc
     1441 cgttcggccc gatcggcacg ccgctgatcg accgcgacga cccggtcggc gtcatcgagg
     1501 atttcttttc catgctggcg cggccgcacc tcaagctgcc gaaggtgttg gtgctgccgg
     1561 atatgcgcct cgacgggccg accgccagcc tgcttcgcat ggcgacggaa gcgcgctcgc
     1621 tgcctttggt caccaccggc gaagtccagc gccctgtcct gcaaagcgaa ctcgacggcg
     1681 acgattacct gaaaaacgcg ctgcgcccgc atcattaccg tgaatttcgc cgcctgaagc
     1741 gccgccttgg cgaaagaggc cacctggagc atatcgtggc gcgcgggccg gagcagatac
     1801 gccacgccat cgaaagcttc ctgacgctgg aagccgccgg ctggaaaggc cgcgagcgca
     1861 ccgccatggc catcgaccgt taccgcgccg ccttcgcgcg agaggcggtg catcgattgg
     1921 ccgaacagga tctgtgccgc atccattcgc ttgtgctcga cgggcgcacc atcgccagcc
     1981 tcatcgtttt cgtcgagcag ggcttcgcct acacctggaa gacggcctat gacgagacgc
     2041 tgtcggcttt ctcgcccgga gcgcttctga tgatcgaggc aaccaggcag catctcgacg
     2101 atccaaacat cacgatgacc gattcctgca cggtgccaga ccacccggtg atgagccggc
     2161 tgtggaccga gcggcggccc gttggcacga ttgtcattgg cctgacgccc gattccgacc
     2221 ggctggcccg gcaggcggca gcacagcttc acctccaccg ggaaacgcgc aacatggcgc
     2281 gcatcgtgcg caaccggatg aaaagcctgc tgagccggcg ctagccaaat cgccccgtcg
     2341 ccggccgaag tcgccggctg aaccgattca gcatccagcc gcccgcaatc ttcacttgaa
     2401 gccttttgtg caacgcgata ggccgttgcc aattcaagaa gcggagcaag cctgtgagcg
     2461 cgcaaaagac cagaaccgaa accgacacat tcggtccgat cgaggtggcg tccgatcgat
     2521 attggggcgc gcaggcccaa cgctcgctgg gcaatttcaa gatcggctgg gaaaagcagc
     2581 ccgcctccat cgtgcgcgcg ctcggcatcg tcaaacgcgc cgcggcggaa gccaatatgg
     2641 acctcgaacg ccttgaccct gcgctgggca agaccatcgt ccaggccgcg caggaggtta
     2701 tcgacggcaa gctcaacgag cattttccgc tggtggtctg gcagacaggc tccggcaccc
     2761 agtccaacat gaacgccaac gaagtcatct ccaaccgagc catcgaattg ctgggcggcg
     2821 tgatggggtc gaaaaagccg gtccacccga acgaccacgt caatatgagc cagtcgtcca
     2881 acgacaccta tccgacggcc atgcacatcg ccagcgccga gcgcatcgtc cacgacctgc
     2941 tgccggccct gaaacacctg catgccgcgc tcgacgccaa ggcgaaggcc ttcgaccaca
     3001 tcgtcaagat cggccgcaca catacgcagg acgccacgcc gcttaccctt ggccaggaat
     3061 tttccggcta tgccgcgcag gtcgcctcat cgatcaagcg catcgagctg accctgcccg
     3121 gcctgtgcga actggcgcag ggcggcaccg ccgtcggcac cggcctcaac gccccgatag
     3181 gctttgccga aaaagtggcc gaccgaatcg ccgccatcac cggcatcgac ttcaaaaccg
     3241 cgcccaacaa attcgaggcg cttgcagccc acgattcgat g
//
LOCUS       DASYCS010000105         2955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2485880,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000105 DASYCS010000000
VERSION     DASYCS010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2485880"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..1791)
                     /locus_tag="VGM46_03745"
     CDS             complement(127..1791)
                     /locus_tag="VGM46_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEY4191736.1"
                     /translation="MSGSVVLIHLAGAVALMLFATRMVKTGVERAYGDVLRHRLRNTM
                     RNPLMAVLAGTGLAIALQSSTAVTLLVGSFAGSGIVSGAAGQLAVRGAEIGSALVTKL
                     LTFDLTLLVPVCLVIGTVMFMATERRDWRQIGRILVGIGLLVLSLEMIGQASEPLRDS
                     QMLPVIITYFAGDPVTAFLLAAFTTWLFQSSIAAILLMAALAGRGFIPPELGVVLVLG
                     VNLGSSIIAPMLTRGADAAVRVVPVGNLLMRGVGSIVMLILFMSFKPPVGFLGATVPD
                     QIVNAHILFNVIILMAGLPLAGLVYRASEKIVALGGKPEPVDAFAATELSALNEAALD
                     TPSQALANATREVVRVCETVEIMLKRIIELYESADADKIKALASLDDRVDRKHAAIKI
                     YLAKVTRNVMTEDEALRCQELIGACVKLEQVGDIIVRNMLVHVKKKFERGLEFTDEGW
                     SELCAFHTSVLANARLAFNVLVSRDPETARQLVLEKDQLRAREKETSASHFVRLRDGT
                     ARSVETSSIHLDTIRDLKQINSLLASMAYPVLEERGLLTGSRLKAS"
     gene            2322..2687
                     /locus_tag="VGM46_03750"
     CDS             2322..2687
                     /locus_tag="VGM46_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006200029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcnB family protein"
                     /protein_id="HEY4191737.1"
                     /translation="MKRLVLAAVAASFLAAPMISAQAAPMVAPIAAPSANVVHVDWKK
                     PNQVQKRKVVVTKKVVRKQQWKRGQRYSDWRRHQAVSDYNRHGLQRPGRGQEWIKVDN
                     NYLLVGIASGVIGAMIAAH"
BASE COUNT          568 a          880 c          955 g          552 t
ORIGIN      
        1 caaaccacgg atgggcgccg ggagcaatcc agaaattgtc gacggcggtt taggttttct
       61 tgagcgcgag ggcgcgtcgg cggtggagcg ccaatctccc ccacgagggg ggagatcggc
      121 agcttctcag ctcgccttca acctcgaccc tgtgagcaaa ccgcgctctt ccagcaccgg
      181 gtaggccatc gacgccagca gtgagttgat ctgtttcagg tcgcggatgg tgtccaggtg
      241 gatggagctg gtctcgacgc ttctggcggt gccgtcgcgc aggcgcacga aatggctggc
      301 gctggtttcc ttctcgcggg cgcgcagctg gtccttttcc agcaccagct ggcgcgcggt
      361 ctcgggatca cgcgacacca gaacgttgaa ggcgagccgc gcattggcga gcaccgatgt
      421 gtggaaggcg cacaattcgc tccatccctc gtcggtgaat tccagcccgc gctcgaactt
      481 cttcttcaca tgcaccagca tgttgcgcac gatgatgtcg ccgacctgtt cgagcttgac
      541 gcaggcgccg atcagttcct ggcagcgcag cgcctcgtcc tcggtcatca cgttgcgggt
      601 gaccttggcg aggtagatct tgatggcggc gtgcttgcgg tcgacgcggt cgtccagcga
      661 ggccagcgcc ttgatcttgt cggcgtcggc gctttcgtac agttcgatga tgcgtttcag
      721 catgatctcg accgtctcgc acacccgcac cacctcgcgc gtggcgttgg ccaaagcctg
      781 gcttggcgta tcgagcgcgg cctcgttcag cgccgaaagc tcggtcgcgg caaaggcgtc
      841 gaccggttcg ggcttgccgc ccagcgccac gatcttttcg gaggcgcgat agaccaggcc
      901 ggccagcggc agcccggcca tcaggatgat gacgttgaag aggatgtgcg cgttgacgat
      961 ctggtcgggc acggtggcac cgaggaagcc gacgggcggc ttgaagctca tgaacaggat
     1021 cagcatgacg atggagccga cgccgcgcat cagcaaattg ccgaccggca ccacgcgcac
     1081 ggcggcatcc gcgccgcgcg tcagcatcgg cgcgatgatc gacgagccga gattgacgcc
     1141 cagcaccagc acgacaccca gttccggcgg aatgaaaccg cgcccggcaa gcgccgccat
     1201 caaaaggatc gcggcgatgc tcgactggaa aagccaggtg gtgaaggctg caagcaggaa
     1261 ggcggtgacc ggatcgccgg cgaaataggt gatgatgacc ggcagcatct ggctgtcgcg
     1321 cagcggctcg gaggcttggc cgatcatttc caacgacagc accagaaggc cgatgccaac
     1381 caggatgcgg cctatctgac gccagtcgcg gcgctcggtc gccatgaaca tgaccgtgcc
     1441 gatgacgagg cagacgggaa ccagcaaggt gaggtcgaag gtgaggagct tagtgaccag
     1501 cgccgagccg atctccgcgc cgcgcacggc aagctggccg gcggcgccgg aaacgatgcc
     1561 cgaaccggca aacgaaccga ccagaagcgt gacggcggtg gaactttgca gtgcgatggc
     1621 cagacccgtg ccggccagaa ccgccatcaa cgggttgcgc atggtgttgc gcaggcgatg
     1681 gcgcagcacg tcgccatagg cgcgctcgac gccggttttc accatgcgcg tggcaaacag
     1741 catcaaggcg accgcgccgg caaggtggat gaggacgacg gatccgctca tgccggcgtc
     1801 ccgcccggca cagcaacagt gccgcatggc cgccgcgaag ccctggcgtg gtagcgagcg
     1861 tgtggattgt tgcctgtcac cttcattcct cgaatcgcaa tcatattagc cggataattt
     1921 tttcttgcag gaaaaaatgc aggccggatg gcgcgcgcgc tggtttttca tatggtgttc
     1981 aactggggtg aaatttcgat aagtcaaggt aatgattaca caaattttat gcttgcactc
     2041 cttccgggcg tcttcggcct ataggcatat tgagtttttc gaagcatgaa ccaccagccg
     2101 aaaggtgtat tttttgctcg atagagtggg tgcgtcgggc cgcatcggcg cgcaggccgg
     2161 ccagccaaag cggcagcatg cggcgggcct gagtgaaggc atccgttata gcggcgctta
     2221 taactgcctg aactgattac aaaaacgtaa tgtagccgtt cagtttcggt tcatggttac
     2281 ggcgctattt ctgcggtcat caacaacgaa ggagtacctc aatgaaacgt cttgttctcg
     2341 ctgccgtcgc tgcgtctttc ctcgccgccc cgatgatctc ggcccaggca gcgccgatgg
     2401 ttgctccgat cgccgcgcca agcgccaatg tcgttcatgt cgactggaag aagcccaacc
     2461 aggtgcagaa gcgcaaggtt gtcgtgacga agaaggtcgt gcgcaagcag cagtggaagc
     2521 gcggccagcg ttactccgac tggcgccgtc accaggccgt cagcgactat aaccgccatg
     2581 gccttcagcg tccgggccgc ggccaggaat ggatcaaggt cgacaacaac tatttgctgg
     2641 tcggcatcgc atcgggcgtc atcggcgcga tgatcgccgc tcactaagcc aggcgcattc
     2701 cggcacgaag gcggctcgca agggccgcct ttttgttttt ggccctgtga ataccgggta
     2761 tggccggcat cgggggtcgt cttgggcggt aggggtgttt atatgaaggg gaaacgagcc
     2821 gttccccatg cgctttccgc ccgcctttct cgatgaaata cgcgaccgag tgccgatttc
     2881 ctcggtggtc ggcacgcgcg tttcgtggga ccgcaagaaa accaatgcgc cgcgcggcga
     2941 tttctgggcc tgctg
//
LOCUS       DASYCS010000106         1966 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_167150,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000106 DASYCS010000000
VERSION     DASYCS010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167150"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..360)
                     /locus_tag="VGM46_03755"
     CDS             complement(37..360)
                     /locus_tag="VGM46_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191738.1"
                     /translation="MTDKQPATPFEPKPVLDLIAGIEADLKRLKDMVETQLCKFDPAN
                     PHNKAPDGKLTEEGVECCYRLFDEGKSRYSVSRQMRISFAAATHRFNTWRQAGGAKRK
                     RTLLG"
     gene            557..1540
                     /locus_tag="VGM46_03760"
     CDS             557..1540
                     /locus_tag="VGM46_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HEY4191739.1"
                     /translation="MLSRVLGFGRRRFDSLSEQEVLALAISSEEDDGRIYRAYADGLA
                     ESFPQSAQLFEAMATEEDGHRAALIDLHRKRFGERIPLIRREHVKGYYERKPDWLVRP
                     LGIEHVRAQAETMERQAYLFYVEAAKRTTDAATRKLLGDLAAAEQRHETLAQHLEKKL
                     VPGDARDEEAATERRQFILTYVQPGLAGLMDGSVSTLAPIFAAAFATHDTWQTLLVGL
                     AASIGAGISMGFTEVASDDGKLSGRGSPIKRGLTVGLMTSLGGLGHALPYLIPDFWTA
                     TVVAAIVVFFELWAIAFIQNRYMQTPFLRAAFQVVLGGALVFVAGVLIGNA"
     gene            complement(1645..>1966)
                     /locus_tag="VGM46_03765"
     CDS             complement(1645..>1966)
                     /locus_tag="VGM46_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006200590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEY4191740.1"
                     /translation="GFSHDFARAMEQAEPDRYTATLSKKARTGKIFIDYLRNGKGSTT
                     VAPYSSRAKKGATVSMPIMWQEVHDGIAPNAFPIGDETTLRRFAQADPWKDFLASAKP
                     LKLK"
BASE COUNT          335 a          595 c          608 g          428 t
ORIGIN      
        1 ttgctgtttg cccaagctga aaaccagccc gaccctctag cccaatagag tgcgcttgcg
       61 cttggcgccg ccggcctgac gccatgtgtt gaagcgatgg gttgcggcgg cgaacgatat
      121 cctcatctgc cgcgacaccg agtaacgcga tttgccttca tcgaacaggc ggtagcagca
      181 ttcgacgcct tcctccgtaa gcttgccgtc gggcgccttg ttgtgcggat tggccgggtc
      241 gaatttgcat agttgcgttt cgaccatgtc cttcaggcgc ttgaggtcgg cctcgatgcc
      301 ggcgatcagg tcgagcaccg gcttcggttc gaatggggtt gcaggctgct tgtccgtcat
      361 gccgcgtcct ttcatttcat tatcagtgga tgtttcgcta tataggccgg cagttgcgga
      421 taaaaagaaa tgggcagcgc cgtatggctc aataattttg aacccgcctt ttttgatcgc
      481 ccgcgccccc ttagggaatt gaccctaggc ctttgcccgc ctagtttaga ataattctaa
      541 actaggataa tcgctcatgc tgtcgcgtgt actcggcttt ggccgccgcc gtttcgattc
      601 gctgtccgag caggaagtgc tggcgcttgc catttcctct gaagaagatg acgggcgcat
      661 ctaccgcgcc tatgccgacg ggcttgctga aagctttccg cagtcggcgc aactgttcga
      721 ggcgatggcg acggaagagg acggtcaccg cgccgcgctg atcgacttgc accgcaagcg
      781 ctttggcgag cgcattccgc tgatccgccg cgagcacgta aaaggctatt acgagcgcaa
      841 gccggattgg ctggtgcggc cgctgggcat cgagcatgtg cgcgcgcagg ccgaaacgat
      901 ggaacggcag gcctatcttt tctacgtcga ggcggcgaag cggaccacgg acgcagccac
      961 gcgaaagctg ctcggcgacc tcgccgccgc cgaacagcgc cacgaaacgc tggcgcagca
     1021 tctcgaaaag aagcttgtgc ccggcgatgc gcgcgacgag gaagccgcga ccgagcgccg
     1081 ccagttcatc ctcacctatg tccagccggg gctggccggg ctgatggacg gttcggtatc
     1141 gacgctggcg ccgatattcg ccgccgcctt tgccacgcac gacacctggc agacgcttct
     1201 ggtggggctt gccgcctcga taggagccgg catttccatg ggcttcaccg aagtcgcctc
     1261 ggatgacggc aagctttccg ggcgcggttc gccgatcaag cgcgggctga cggttggcct
     1321 gatgacatcg ctgggcggcc tggggcacgc cctgccctac ctgatcccgg atttctggac
     1381 ggcgaccgtc gttgccgcga tcgtggtgtt cttcgaatta tgggccatcg ccttcatcca
     1441 aaaccgctac atgcagacgc cgttcctgcg cgcagccttc caggtggtgc tgggcggcgc
     1501 gctggtgttc gtcgccggcg tgctgatcgg caacgcctag cgcggttctg gtttttaccg
     1561 aatcggtaaa accgctctat ctctttgttt tggccgcatt tgcggacgca aaaccgcttc
     1621 acgcttttgc tggaaatgct ccagctattt cagcttcagc ggcttggcgc tggccaagaa
     1681 atccttccac ggatcggctt gtgcgaaccg ccgcagcgtc gtctcgtcgc cgatgggaaa
     1741 ggcgttgggc gcgatgccgt catgcacttc ctgccacatt atcggcattg aaaccgtcgc
     1801 gcccttcttg gcgcgcgacg aatagggcgc aaccgtggtc gaacccttgc cgttgcgcag
     1861 gtagtcgatg aagattttgc cggtgcgcgc ctttttcgac agggtcgcgg tgtagcggtc
     1921 gggttcggcc tgctccatgg cgcgggcgaa atcgtgcgaa aaacct
//
LOCUS       DASYCS010000107         8644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2195443,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000107 DASYCS010000000
VERSION     DASYCS010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 8644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8644
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2195443"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VGM46_03770"
     CDS             <1..225
                     /locus_tag="VGM46_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008840650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEY4191741.1"
                     /translation="TSLWLEGPRGTDARGLAEIAATRGVLIEPGDRFFDQGNAPSRFM
                     RLGISSIALQHIEPGIRELATAAGRRPAAA"
     gene            432..1988
                     /locus_tag="VGM46_03775"
     CDS             432..1988
                     /locus_tag="VGM46_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013527999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEY4191742.1"
                     /translation="MSLAKPAAAERGPRAFTDATAQPLDSREQSRPVPNYRPLLPNDL
                     ERIHEAALVLLETVGFANPIPSCVEALTGAGAIYGEDGRIRFPRRLVLETVAGAARHF
                     TLHGQHPRHDMAIQGGRVHYGTAGLAVHLVDAEKRSYRESALNDIYDAARIVEGLDNI
                     HFFQRPMVARDLADPLELDLNTLYACVSGTSKHIGTSFTVRENVKPALDMLHMIAGGE
                     AAFRARPFISSSSPFVVSPMKFAEDACGVLEECVKGGIPILLLSTGQAGATAPAALAG
                     AVVQAVAEVLAGLAYVNALKRGHPAMFGTWPFASDLRTGQMSGGSAEQALLTAACAQM
                     AQFYDLPGASAAGMADSKLPDIQSGYEKGITNVMAGLSGLDLVYEAAGMHASLLGFCL
                     ESLIIDNDMLGYCQRCARGIDTGDAALSVEAITDVCLKGPGHYLNRGAPAGEGRPDAS
                     YPMIGDTLTPKEWCDKGRPDMLQKAIAEKKRILAERFPRHIVKSLDDRLRARFGELIR
                     LPRGAMGWKP"
     gene            2042..2581
                     /locus_tag="VGM46_03780"
     CDS             2042..2581
                     /locus_tag="VGM46_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018426296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY4191743.1"
                     /translation="MTPLDISKLLVRTSMKDRAAFDLLYRQTSAKLFGVCLRVLNDRT
                     EAEEALQEVFVKVWTKADRFAVSDLSPISWLVAVARNHSIDRIRARRPTAADIDAALD
                     VADTAPDPEAATMADDDSERIHRCLGELEKDRAAAVRGAYLKGESYAELAQRHGVPLN
                     TMRTWLRRSLLKLRECLER"
     gene            2578..3294
                     /locus_tag="VGM46_03785"
     CDS             2578..3294
                     /locus_tag="VGM46_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEY4191744.1"
                     /translation="MTALETNGPEHEGDDLLAAEYVLGVLTADERLAAQRRAEADTAF
                     ARLVDAWEVRLSPLDGGYAEVEAPASVKAALDRRLFTPTSAGAARPGLWSSLAFWRGL
                     TMAALAALALYVAAPLVNPPVPSSQPTLVAALAADGSDVKYLAVYDPAQHSVGLSHVA
                     GEHATGKDFELWMIEGKNPPVSMGIIAAGATARLPVSPAVQQRLAQGAVLAVSLEPAG
                     GSPTGQPTGPVVAAGDLKNI"
     gene            3401..3958
                     /locus_tag="VGM46_03790"
     CDS             3401..3958
                     /locus_tag="VGM46_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HEY4191745.1"
                     /translation="MRKFATVVFAGAVALSALGTVAYAENPHVGGAAMFADKNIVENA
                     VNSKDHTTLVAAVKAAGLVDTLQGKGPFTVFAPTNEAFAALPAGTVDTLLKPENKDKL
                     VKILTCHVVAAKAMSTDVMKMVKDDGGMHKVKTVGGCELTLKDEGGKVTVTDENGNVA
                     NVTIADVEQSNGVIHVVDKVLLPKM"
     gene            4160..4663
                     /gene="msrB"
                     /locus_tag="VGM46_03795"
     CDS             4160..4663
                     /gene="msrB"
                     /locus_tag="VGM46_03795"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013891876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEY4191746.1"
                     /translation="MNRRDFLLSGAAGVALVVGGSALLRMGKPEAAQAAEKFAVTKTD
                     AEWRAILSDEAFNVLRREGTEYPGTSPLLNEHRKGTFACAGCDLPVYSSETKFESGTG
                     WPSFWQALPNGIATREDSTLGMVRIEAHCSRCGGHLGHVFDDGPAPTGKRHCIDGVAL
                     NFKPAVA"
     gene            complement(4692..5351)
                     /gene="yihA"
                     /locus_tag="VGM46_03800"
     CDS             complement(4692..5351)
                     /gene="yihA"
                     /locus_tag="VGM46_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006325754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEY4191747.1"
                     /translation="MNALYKSTVEVGLFTRAWIFIRGVPAMKFLPPEGPPEIAFAGRS
                     NVGKSSLINALVNQKGLARTSNTPGRTQELNYFVPDGFSGEGADLPPLALVDMPGYGY
                     ATAPKEKVDEWTKLVFDYLKGRVTLKRVYVLIDARHGIKAKDEEVLSLLDKAAVSYQI
                     VLTKTDKIKVAGVPRLIEETLLKIKKRPAAFPFVLATSSEKSEGLDEVRAAIVLAANG
                     G"
     gene            complement(5467..7278)
                     /gene="yidC"
                     /locus_tag="VGM46_03805"
     CDS             complement(5467..7278)
                     /gene="yidC"
                     /locus_tag="VGM46_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEY4191748.1"
                     /translation="MQNNRNFFITIALSVLILTLWQVFYMNPRIETQREAAKVEAARV
                     EANKPADAAASGAAAQAPQPGAIPNAPGTAEAMAASREQALASTGRVKIDTPSLSGSI
                     NLTGARFDDLGLKHYTETVDKSSPEIVLLNPSALPAGYFAEIGYSGSPEAGKLPGPDT
                     VWTADGNQTLTATTPVTLTYTNDKGLVFKRTVSVDENYMFTVDDTVTNSGAAAVSLAN
                     YGRVTRFEKPDDRSTYVLHEGLIGVTGQEGLEEIKYSKIEKDKQVTPGKSNDGWLGIT
                     DKYWAVALVPSAKQPFQPRYGYFEDGRTRYQSDYLTDQITVAPGQSTTLETMIFAGAK
                     EVSRINAYEKDRQIRQFDLLIDWGWFYFITKPMFWLIDTLYKFFGNFGLAIITTTVLV
                     KALFFPLANKSYSSMANMKKVQPKMTEIREKYADDKMAQQKAMMELYKTEKINPIAGC
                     WPMLIQVPVFFALYKVLYITIEMRHAPFFGWIHDLASPDPTSLFNLFGLLPFAPPAFL
                     PHLGVWAVIMGITMFLQMRMNPTPPDPTQAMIFTWMPVVFTFMMGSFPAGLVIYWAWN
                     NTLSIIQQGIIMKRQGVKVELWDNLAGMFRKKPSPAK"
     gene            complement(7292..7645)
                     /gene="rnpA"
                     /locus_tag="VGM46_03810"
     CDS             complement(7292..7645)
                     /gene="rnpA"
                     /locus_tag="VGM46_03810"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEY4191749.1"
                     /translation="MPAAGKPVGKTPGRLLKRAEFLAVRRGEKRRGRLFLVEVLDRGD
                     GGVARVGFTVTKKVGNAVVRNRVRRRLKEAVRTHAADDMTPGSDYVIVGREEVLSAPF
                     GLLKAELSRRIRATR"
     gene            complement(7659..7793)
                     /gene="rpmH"
                     /locus_tag="VGM46_03815"
     CDS             complement(7659..7793)
                     /gene="rpmH"
                     /locus_tag="VGM46_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005980833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEY4191750.1"
                     /translation="MKRTYQPSKLVRKRRHGFRARIATKSGRAVIAARRNRGRKRLSA
                     "
     gene            8066..>8644
                     /locus_tag="VGM46_03820"
     CDS             8066..>8644
                     /locus_tag="VGM46_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006200653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY4191751.1"
                     /translation="MNKQGSEDDQAGAPPPITRRVPLARGLSVKLLLFTIAFGLLAEI
                     LIFLPSIANYRVTWLKERLGTAAAVSVLLVQGDPQSLSQAAQNDVLMAIGAKAIAVRD
                     GGVSRLLAIAEMPPQVDEHIDLAHIGMIDEITGALDTLASGGNRMLRVFGPVDDNPKE
                     FELIMPDYRLRAAMLAYSRTVAFVSLLISLFTA"
BASE COUNT         1554 a         2741 c         2743 g         1606 t
ORIGIN      
        1 acctcgctgt ggctggaagg cccgcgcggc accgacgcgc gcggactggc ggagatcgcc
       61 gccacgcgcg gcgtgctgat cgagccgggc gaccgcttct tcgaccaggg caacgcgccc
      121 tcgcgcttca tgcggctcgg catctcgtcg atcgcgctcc agcacatcga acccggcatc
      181 cgcgaactgg cgacggcagc ggggcgcagg ccggccgccg cctgacaggc cgcgccacaa
      241 tgtatatagg ccggcccgaa catgctggcc cgaactggcc ccatccgaac attggcgacg
      301 tccgaataag gctggcccat gcggcgaagt gcgctgggcc ttaggaccac tcccaaggcg
      361 cggcatagtg ggtggcgaaa atgcagcgcg ccatcgggcc agcccgccga acgcgcccgc
      421 agggagtatc catgtcgctt gcaaaaccgg ctgcggccga aagagggccg cgcgcattca
      481 ccgacgccac ggcccagccg ctcgacagcc gggaacagag ccggcccgtg ccgaactacc
      541 gcccgctcct tcccaacgac ctcgaacgca ttcacgaggc ggcactggtg ttgctggaaa
      601 cggtgggctt cgccaatccg atcccctcct gtgtcgaagc gttgaccgga gccggggcga
      661 tctatggcga ggacggccgc atccgctttc cgcgccggct ggtgcttgag actgtcgcgg
      721 gcgctgcgcg ccactttacg ctgcatggcc agcatccccg gcacgacatg gcgatccagg
      781 gcgggcgcgt gcactacggc acggcggggc ttgccgttca cctggtcgac gccgaaaagc
      841 gcagctatcg cgaatccgcg ttgaacgaca tttacgatgc cgcccgcatc gtcgaggggc
      901 tggacaatat ccacttcttc cagcgcccga tggtggcgcg cgacctggcc gatccgctcg
      961 aactggacct caacaccctt tatgcctgcg tgagcggcac gtcgaagcat atcggcacct
     1021 cgttcacggt gcgcgagaat gtgaagccgg cgctggacat gctgcacatg attgccggcg
     1081 gcgaggcggc gtttcgcgcg cggccgttca tttcaagctc aagccctttt gtcgtgtcgc
     1141 cgatgaagtt cgccgaagac gcctgtggcg tgctggagga atgcgtcaag ggcggcattc
     1201 ccatcctgct gctttcaacc ggccaagccg gcgccacggc gcccgccgcc ctggctggcg
     1261 cggtggtgca ggcggtggcc gaagtgctgg cggggctggc ctatgtcaat gcattgaagc
     1321 gcgggcatcc ggccatgttc ggcacctggc cgtttgcgtc cgaccttcgc accgggcaga
     1381 tgtccggcgg ctcggccgag caggcgctgt tgacggctgc ctgcgcgcag atggcgcaat
     1441 tctacgacct gcccggcgct tccgccgccg gcatggccga ttccaagctg cccgacatcc
     1501 agtccggcta tgaaaaaggc atcaccaatg tcatggccgg gctttccggc ctcgaccttg
     1561 tttacgaggc ggcaggcatg catgcctcgc tgcttggctt ctgcctcgaa agcctgatca
     1621 tcgacaacga catgctgggc tattgccagc gttgcgcgcg cggcatcgat accggcgatg
     1681 ccgcgctttc ggtggaggcc atcaccgatg tctgcctgaa agggccgggc cactatttga
     1741 accgtggcgc gcccgccggc gagggccgcc cggatgcgtc ctatccaatg atcggcgata
     1801 cgctgacccc gaaggaatgg tgcgacaagg gccggcccga catgctgcaa aaggcgattg
     1861 ccgagaaaaa gcgcatcctt gccgagcgtt ttccacgtca catcgtgaaa tcgctcgatg
     1921 accggctgcg cgcccgcttc ggcgagctta tcaggctgcc gcgcggcgcg atgggctgga
     1981 agccctagca acggcgccaa gcgctgttgc aaattgccgc cagaacccta gcttgggtcg
     2041 catgacgcct ctggatatca gcaagctcct ggtccgcacc tcgatgaagg accgcgcggc
     2101 cttcgacctg ctctatcggc agacgagcgc gaaacttttc ggcgtctgcc tgcgtgtctt
     2161 gaacgacaga acggaagccg aggaagcgct tcaggaggtt ttcgtcaagg tatggaccaa
     2221 ggcggatcgt ttcgcggtgt cggatctcag cccgatctcg tggctggtgg ccgttgcccg
     2281 caaccattcg atcgaccgca tccgggcgcg gcggccaacg gcagccgata tcgatgcggc
     2341 gctcgacgtg gccgatacgg cgcccgatcc tgaagccgcg acgatggcgg acgacgacag
     2401 cgaacgcatc caccggtgtc ttggcgaact ggaaaaagat cgggcggcag ccgtgcgcgg
     2461 ggcttatctc aagggcgaaa gctatgccga actggcgcag cgtcatggcg taccgctgaa
     2521 cacgatgcgg acatggctgc ggcgcagcct gttgaaatta cgggaatgcc tggaaagatg
     2581 acggcgttgg aaacaaacgg accggagcat gagggagacg acctgctggc cgccgaatat
     2641 gttctcggcg tgctgacggc ggacgagcgt ctcgcagccc agcgtcgcgc cgaggccgac
     2701 accgcctttg cgcggcttgt cgacgcctgg gaagtgcggc tttcgccgct tgacggcggc
     2761 tatgccgagg tggaagcgcc agcttcggtg aaggcggcgc tggaccggcg gctgtttacg
     2821 cccacctctg ccggcgccgc gaggcctggc ctgtggtcca gccttgcgtt ctggcgcgga
     2881 ttgacgatgg cagcccttgc cgcgctcgcg ctttatgtcg ccgcaccgct ggtcaatccg
     2941 cccgttccct cgtcgcaacc aacgctggtg gcagcgcttg ccgccgatgg cagcgacgtc
     3001 aaatatctcg ccgtctacga tcccgcacag cacagcgtcg gtctttcgca tgtggcgggc
     3061 gagcatgcga ccggcaagga tttcgaactg tggatgatcg agggcaagaa cccgccggtt
     3121 tcgatgggca tcatcgccgc cggcgccacc gcgcgcctgc ccgtctcgcc tgcggtccag
     3181 caaagactgg cgcagggcgc ggtgctggcc gtcagcctcg aaccggccgg cggctcgcca
     3241 accggccagc caacggggcc ggtcgttgca gccggcgacc tgaagaatat ctgacagtcc
     3301 ggcctttctt cgacgccttc tgaaactctg cggattgcgc gccgtatctc cttgtgcccc
     3361 agcgaaactg ggaaacagaa aactcaagga gtttgacacc atgcgtaaat tcgccaccgt
     3421 tgtgttcgcc ggggctgtcg ccctttccgc tctgggcacc gttgcctatg ccgaaaaccc
     3481 gcatgtcggc ggggcagcga tgtttgccga caagaacatc gtcgagaacg ccgtcaattc
     3541 gaaggatcac accacgctgg tggcagccgt aaaggcagcc ggtctggtcg atacgttgca
     3601 gggcaaaggc ccgttcaccg tgttcgcacc gaccaacgag gccttcgcgg ctctgccggc
     3661 cggcacggtc gatacgctct tgaagccgga aaacaaggac aagctggtca agatcctgac
     3721 ctgccacgtc gtcgccgcca aggcgatgtc gaccgatgtg atgaagatgg tcaaggacga
     3781 tggcggcatg cacaaggtca agaccgtcgg cggctgcgaa ctgacgctga aggatgaagg
     3841 cggcaaggtc accgtcaccg acgagaacgg caatgtcgcc aatgtgacca tcgccgatgt
     3901 cgagcagtcg aacggcgtca tccacgtcgt cgacaaggtt cttctgccga agatgtagcc
     3961 ggcccttcag ttaggcttcg cggaataagt ccctcttcgc ggagccgagc ctcgcgccga
     4021 ccatcgcaag cccgtttcaa ccttcctata tgaagcggga gccggcgcga ggcgacttca
     4081 agctaatgga cctctcaccc gtttttgatt tccccgtgac gcgcatctct gtcaaaaacc
     4141 agcctggagg tactgaaaca tgaaccgtcg tgatttcctg ttgagcggtg ccgccggcgt
     4201 ggctctcgtg gttggcggct cggcgctgtt gcgcatgggc aagccggaag cggcgcaggc
     4261 ggccgagaaa ttcgccgtca ccaagaccga cgccgagtgg cgcgccatcc tttccgatga
     4321 agccttcaac gtattgcgcc gcgagggaac cgaatatccg ggcaccagcc cgctgctgaa
     4381 cgagcatcgc aagggcacat tcgcctgtgc cggctgcgac ctgcccgtct actcgtccga
     4441 gacgaagttc gagtccggca ccggctggcc gagcttctgg caggcgctgc ccaacgggat
     4501 cgccacgcgc gaggattcca ccttgggcat ggtgcggatc gaagcccatt gcagccgctg
     4561 cggcggccat ctcggccacg tcttcgacga cggcccggcg ccgaccggca aacgtcactg
     4621 catcgacggc gtggcgctca acttcaagcc ggccgtcgcc tagataggcc tgcgggcggc
     4681 tggatcagac ctcagccgcc attggcggcg aggacgatcg ccgcgcgcac ctcatccagc
     4741 ccttcggact tttccgacga agtcgccagc acgaacggga aggcggccgg acgcttcttg
     4801 atcttcagaa gcgtctcctc gatcagccgg ggcacgcccg ccaccttgat cttgtcggtc
     4861 ttggtcaaga cgatctggta ggacacggcg gccttgtcca gcagcgaaag cacttcctcg
     4921 tccttggcct tgatgccatg gcgcgcgtcg atcagcacgt agacgcgctt cagcgtgacg
     4981 cggcccttga gataatcgaa gaccagcttc gtccactcgt cgaccttctc cttcggcgcg
     5041 gtggcataac cgtagccggg catgtcgacc agcgccaacg gcggcagatc ggcgccctcg
     5101 cccgaaaagc cgtccggcac gaaatagttg agttcctgcg tacgccccgg cgtgttcgac
     5161 gtgcgcgcca ggcccttctg gttgaccagc gcgttgatga gcgacgactt gccgacattg
     5221 gagcgacccg caaaggcgat ctccggcggc ccttccggcg gcaggaactt catcgccggc
     5281 acgccgcgta tgaatatcca tgcccgcgtg aacaggccaa cttcgacagt ggatttatac
     5341 agggcgttca aatcgcggcc tccggccagg tctggttgaa tggcctgcct atcatcattc
     5401 cggtaagccg tcagcaaaag ccccggacgg accggggctt ttgggtttat gcgggcatat
     5461 ggccggctat tttgccggtg agggcttttt tcggaacatc ccggccaggt tgtcccacaa
     5521 ttcgaccttg accccctgcc gcttcatgat gatgccctgc tggataatcg acagcgtgtt
     5581 gttccacgcc cagtagatga cgaggcctgc cgggaacgaa cccatcatga aggtgaagac
     5641 caccggcatc caggtgaaga tcatcgcctg tgtcgggtcc ggcggcgtcg ggttcatgcg
     5701 catttgcagg aacatggtga tgcccatgat gactgcccag acgccgagat gcggcaggaa
     5761 ggccggcggc gcgaacggca gcaggccgaa taggttgaac agcgaagtcg gatcgggcga
     5821 ggcaagatcg tgtatccagc cgaagaacgg cgcgtgccgc atctcgatgg tgatgtaaag
     5881 caccttatag agcgcaaaga agaccgggac ctggatcagc atcggccaac agccggcgat
     5941 cggattgatt ttctccgtct tgtacaattc catcatggcc ttttgctggg ccatcttgtc
     6001 gtccgcgtat ttctcgcgga tttccgtcat cttcggctgc accttcttca tgttcgccat
     6061 cgacgaatag gacttattgg cgagcgggaa gaacagggcc ttgaccagaa cggtggtggt
     6121 gatgatggcg aggccgaaat tgccgaagaa cttgtagagc gtgtcgatca gccagaacat
     6181 cggcttggtg atgaaataga accagcccca gtcgatcaaa aggtcgaact ggcggatctg
     6241 ccggtccttc tcataggcgt tgatgcggct gacttccttg gcgccggcga agatcatggt
     6301 ctccaacgtc gtcgactggc cgggcgcgac cgtaatctgg tcggtcagat aatccgactg
     6361 gtagcgcgtg cggccatcct cgaaatagcc atagcggggc tggaaaggct gcttggccga
     6421 cggaaccagc gccaccgccc agtatttgtc ggtgatgccg agccagccgt cattggactt
     6481 gccgggcgtg acctgcttgt ccttctcgat cttggaatat ttgatttcct caagcccctc
     6541 ctggccggtc acgccgatca ggccttcatg caggacatag gtgctgcggt cgtccggctt
     6601 ctcgaagcgc gtcacgcggc cgtaattggc gagcgagacg gcggccgcgc cggaattggt
     6661 caccgtgtcg tccacggtga acatgtagtt ttcatcgacg gaaacggtgc gcttgaaaac
     6721 caggcccttg tcgttggtgt aggtcagcgt aaccggcgtc gtcgccgtca gcgtctggtt
     6781 gccgtccgcg gtccataccg tatcgggtcc cggcagcttg ccggcttccg gcgaaccgga
     6841 atagccgatt tcggcgaaat agccggcggg cagcgccgat gggttgagca gcacgatttc
     6901 cggtgaactc ttgtcgacgg tctcggtgta atgcttgagg ccgagatcgt cgaagcgcgc
     6961 gccggtaagg ttgatggagc cggaaaggct cggcgtatcg atcttgacgc ggccggtcga
     7021 agccagcgcc tgctcgcggc tggcggccat cgcctcggcc gtgcccggcg cgttggggat
     7081 ggcgcccggc tgcggcgcct gcgcggcggc gcctgaagcg gcggcatctg ccggcttgtt
     7141 cgcctcgaca cgcgctgcct cgaccttggc ggcctcgcgc tgcgtttcga tgcgcgggtt
     7201 catgtagaac acctgccaca aggtcaggat cagcaccgac agcgcgatgg tgatgaagaa
     7261 gttgcgattg ttttgcatcg aggaccgtgg cctatcgtgt tgcgcgaatt cggcgggaaa
     7321 gttcagcctt caacaaaccg aacggagcgg aaagcacttc ttcgcgcccg acgatgacat
     7381 agtcactgcc cggcgtcatg tcatcggccg cgtgcgtgcg cacggcctct ttcagtcgcc
     7441 gcctcacgcg gttgcggacc accgcgttgc cgaccttctt ggtgaccgtg aagccgacgc
     7501 gcgccacccc gccgtcgccc cggtcgagga cttcaacgag aaaaagccgc ccgcggcgct
     7561 tttcgccacg ccggacagcc aggaattcag cacgcttcag aagccgtccg ggagttttcc
     7621 caactggctt tccggcagcc ggcaacggtc ttttccgctc aggcgcttag acgcttgcgg
     7681 ccacggttgc ggcgggctgc aatgacggcg cggccgctct tggttgcgat gcgcgcgcga
     7741 aagccgtgcc ggcgtttgcg gacgagtttg gatggctggt aggtacgctt catttgttta
     7801 ataccgcgga gtgcggccct tcttgaatct gccgcttatg caacaggagc tttggctggg
     7861 ccagtcttgg cgcgatcgaa accgcgccgg gttgcaaggc ccgagcgtga gcgggcttat
     7921 agaaagaagg tttttggaag tcaatccgat cggccccact tggccgcggc acgactgccg
     7981 gcgacgaagt tttgcgtaac aattggcaga ttggccgcaa tcgcctaatt aaaccacatt
     8041 atatcggaaa tggcattcgc cacgcatgaa taaacaaggc tcagaggatg accaagccgg
     8101 cgcgccgccg ccgatcacgc gccgcgtgcc gctggcgcgg gggctttcgg tcaagcttct
     8161 cttgttcacc atcgcgtttg ggcttctcgc cgaaattctc atcttcctgc cctccatcgc
     8221 caattaccgt gtcacctggc tgaaggaacg gcttggcacc gccgccgccg tttccgtcct
     8281 tctggtgcag ggcgacccgc aaagcctttc acaggcggcg caaaacgacg tgctgatggc
     8341 cattggcgcc aaggccatcg ccgtgcgcga cggcggcgtt tcgcggctgt tggccattgc
     8401 cgaaatgccg ccacaggtgg acgagcatat cgacctcgcc catatcggca tgatcgacga
     8461 aataaccggc gcgctcgata cgctggcgtc cggcggcaac cggatgctgc gcgtttttgg
     8521 cccggttgac gataatccca aggaattcga gctgatcatg ccggattacc ggctgcgcgc
     8581 cgccatgctg gcctattcgc gcacggtcgc cttcgtctcg ctgctgatct cgctgttcac
     8641 cgcc
//
LOCUS       DASYCS010000108         5846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3792161,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000108 DASYCS010000000
VERSION     DASYCS010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 5846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5846
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3792161"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="VGM46_03825"
     CDS             complement(<1..671)
                     /locus_tag="VGM46_03825"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007007565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEY4191752.1"
                     /translation="MTGYLSSPFARRVSVGVDVGGVMVGGGAPVVVQSMTNTDTADID
                     QTVAQVAALHRAGSEIVRITVDRDESAAAVPKIRERLERLDVHVPLVGDFHYIGHKLL
                     TDHPACAEALAKYRINPGNVGFKDKRDRQFADIVEMAIRHDKPVRIGVNWGSLDQELL
                     TRLMDDNQQRGFPQTAGQVMREAIVQSAILSAEMAEEIGLAREKIILSAKVSGVQDLI
                     AVYAEL"
     gene            1065..2339
                     /locus_tag="VGM46_03830"
     CDS             1065..2339
                     /locus_tag="VGM46_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4191753.1"
                     /translation="MTATTDIENERTMEPGSQPAIAIASIILSMGLIAVGNGLMFAYI
                     PVRLGAENYDPVWAGLIVTGLSAGGLAGCMLTGPLVRRVGHARGFMVLSALIALSNAA
                     VGAGTYPVAWIAARALYGFAICGLFIVAQSWLNDVVANAVRGRVMAVFSVAYIAGLGV
                     GYSLLALVDITGAEGPLIGIAFTALSILPVGLTRLAQPAAPRAASIALRQAWRISPVG
                     IAGMLAVGGLSMSISGFAPIHATAKGYSQDDVAMLLAAMPVGTLILQIPLGWISDRVD
                     RRFVLVAASALAVAAGALAIGFDGRSLALLIVIYLVWDGASESIYSLASAHANDRTGK
                     EDMVALSSSLLFAWALAGFVVPGVVTGLSVVFGTQSFIFVSIAIAAAFCLFVIWRIIL
                     VRPVPIAETGLFSPMAGQAPLSVELAFSEAES"
     gene            2581..2958
                     /gene="rsfS"
                     /locus_tag="VGM46_03835"
     CDS             2581..2958
                     /gene="rsfS"
                     /locus_tag="VGM46_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006327558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEY4191754.1"
                     /translation="MPSPAGISVNDAASRAIDTVLASLDDSKAENIVSIDLQGKSSLG
                     DYMVIASGRSHRHVAAVADHILKALKDAGLGQARVEGLPGADWVLIDSGDVIVHVFRP
                     EVRDFYNLEKMWQTPDIEEETLH"
     gene            2987..3469
                     /gene="rlmH"
                     /locus_tag="VGM46_03840"
     CDS             2987..3469
                     /gene="rlmH"
                     /locus_tag="VGM46_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006206389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEY4191755.1"
                     /translation="MKLVVHAVGRLKAGPERELADRYFKRLAKSGPGVGIEFSDIVEI
                     PEGHAQTANERRREEGQRLQTHIQPGTALILLDERGKNLSSEDLAGKIGLLRDGGRKA
                     VLVAIGGADGHDASLHKQAELVLSFGALTWPHQLVRVMLAEQLYRVATILAGHPYHRS
                     "
     gene            3588..4937
                     /locus_tag="VGM46_03845"
     CDS             3588..4937
                     /locus_tag="VGM46_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HEY4191756.1"
                     /translation="MSEGRKSIIGFWRGGACAAAALMLLARPALAQDSTAQTPDPDQN
                     RAEYESVSHEITLSSERLAKLAADIATVKQDHASITAALIQSAKTEQKLGQDIEDIGG
                     RLEGLKGQEGTIRASLAKRRDVLAEVLGALQRMGINPPPAILVKPEDALSSVRSAILL
                     GAVVPELRAQTDILVSQLKELTRVTASIEAERARLTATVSEQAAEKQRLTMLLEAKRK
                     LQADTESAMAEEGRKSQDLAARAASLKELIASLEKDADKKRSANDAARAAAEQAAANA
                     GTQAEADAAKAQLASLAVPEANQLAAAAPFSALRGQIALPVIGKVKRRFGADDGNGMA
                     MLGDMVATQSGSIVTAPADGKVLYAGPFRSYGQLLILDAGDGYHVVLAGMTRLNVASG
                     QSVLAGEPIGAMGEARVASASASLDENSTPELYVEFRKDGKPVDPAPWWADRFSGRT"
     gene            4940..>5846
                     /locus_tag="VGM46_03850"
     CDS             4940..>5846
                     /locus_tag="VGM46_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY4191757.1"
                     /translation="MRKLSLLFAGALMGASAMSLVYGAPGSTANAAASDTYKQLAIFG
                     DIFERVRAQYVTPPDDKALIENAINGMLTSLDPHSSYMNAEAAQDMRVQTKGEFGGLG
                     IEVTMENDLVKVITPIDDTPAAKAGVLAGDYIAKIDGEEVRGLTLNDAVEKMRGLVNT
                     PIKLTILRQGADKPIELTVMRDIIKVKAVKFRVENDIGYMKITSFTEKTYDDLENAID
                     TIKKQVPNDKLKGYVLDLRLNPGGLLDQAVSVSDAFLDRGEIVSTRGRDPKDVTRFDA
                     RPGDDINAKPLIVLVNGGSASASEIV"
BASE COUNT         1039 a         1748 c         1974 g         1085 t
ORIGIN      
        1 agctcggcat agacggcgat caagtcctgc acgccgctga ccttggccga caggattatc
       61 ttttcccgcg ccagcccgat ttcctcggcc atttcggcgg agaggatggc cgactgcacg
      121 atggcttcgc gcatcacctg cccggcggtc tgggggaagc cgcgctgctg gttatcgtcc
      181 atcagccggg tcagcagttc ctggtcgagc gaaccccagt tgacgccgat gcgcaccggc
      241 ttgtcgtggc ggatggccat ttcgacgatg tcggcgaatt ggcggtcgcg cttgtccttg
      301 aagccgacat tgccggggtt gatgcggtat ttcgccagcg cttcggcgca ggccggatgg
      361 tcggtcagaa gcttgtggcc gatataatgg aagtcgccaa ccagcggcac atggacgtcg
      421 aggcgttcga ggcgctcgcg gattttcggc acagccgccg cgctctcgtc gcggtccacg
      481 gtgatgcgca cgatttcgga gccggcgcga tgaagggcgg ccacctgggc cacggtctgg
      541 tcgatatcgg cggtgtcggt gttggtcatg gattgcacca cgaccggcgc tccgccgccg
      601 accatgacgc cgcctacatc gacgccgacg gaaacgcggc gtgcaaaggg agaggaaaga
      661 tatccggtca tgccggcggc ctcacgtctg tttcaatacg cccatgatag tttgacggca
      721 ggggcgccga tagcatttcg attgtcgcat gcccgcatgt aggggatgtg aacgccgccc
      781 tggcgcggtt caggctcgtc ttcgcatgag gtggaggacg aaggccgggt tgtcaatggc
      841 ccggttgggg cgagattggg gcggaggctg ggatgcaggg tgggcggcta tcgggccgga
      901 cgacaggctg gacgccgggt cctgcgattt ccggcattcg catcgtgacg gcaagggctt
      961 cgggcaaatg gttgcattgc catgccgccc gcgcggtgca gagtggtctg gacatgtcgc
     1021 ttttacagtg gctcgcgcat ggggcagcgg gcaatctgtg ggcgatgaca gcgaccaccg
     1081 acatagagaa cgagcggacg atggaacccg gcagccagcc ggcgatcgcc atcgccagca
     1141 tcatcttgtc gatgggtttg atcgcggtcg gcaacggcct gatgttcgcc tatatcccgg
     1201 tgcggctcgg cgcggaaaat tacgatccgg tatgggcagg gctgatcgtc accggcctca
     1261 gcgccggggg cctggccggc tgcatgctga ccggtccgct ggtgcggcgc gtcggtcatg
     1321 cgcgcggctt catggtgctg tcggcgttga tagccctgtc caacgccgcg gtcggcgcgg
     1381 gaacctatcc ggtggcgtgg atcgcggcgc gggcgcttta cggctttgcc atatgcgggc
     1441 tgttcatcgt ggcgcagagc tggctgaacg acgtggtcgc caacgcagtg cgcggccggg
     1501 tgatggcggt gttttcggtc gcctacattg ccgggcttgg cgtcggctat tcgctgctgg
     1561 cgctggtcga catcaccggc gcggaagggc cgctgatcgg catcgccttc accgcgctgt
     1621 cgatcctgcc ggtcgggctg acccggctgg cgcagccggc cgcgccgcgc gccgcttcaa
     1681 tcgcgcttcg gcaagcctgg cgcatatcgc cggtcggcat tgccggaatg ctggcggtgg
     1741 gcgggctttc catgtcgatc tcgggttttg cgccgatcca tgccacagcc aagggctaca
     1801 gccaggacga tgtggcgatg ctgctggcgg cgatgccggt cggcacgctc atcttgcaga
     1861 taccgcttgg ctggatatcg gacagggtgg accggcgctt cgtgctggtg gctgcctccg
     1921 cgctcgccgt cgcggcaggg gcgctggcca tcggcttcga cgggcgctcg ctggcgctgc
     1981 tcatcgtcat ttatctcgtc tgggacggcg cgtcggaatc gatctattcg ctggccagcg
     2041 cgcacgccaa cgaccgcacc ggcaaggaag acatggtggc gctatcgagt tcgcttctgt
     2101 ttgcgtgggc gcttgcgggt ttcgtcgtgc cgggggtcgt cactggcctg tcggtggtgt
     2161 tcggcacaca gtccttcatt ttcgtctcca tcgccattgc cgccgccttc tgcctgttcg
     2221 tcatctggcg gatcattctg gtgagaccgg tgccgattgc cgaaacgggg cttttctcgc
     2281 cgatggccgg gcaagcgccc ctctcggtgg agctggcctt ttcggaggct gaaagttgac
     2341 gattgtcgcc tggcggtcga tggccgaatc gttccgctcg gccaagcagt tccgacattt
     2401 tctgctgtgg cacggaacag acagtgacga acttcgttgc ctgcttcggc ggggcctgtc
     2461 ttgaaactgc aatgcgactc tgcctatcta agcgatatca ccgcatttca tggtgatctg
     2521 gttgttcttg ctgcgaaagg aaagacactg agaacagcac ttcggaagaa ggccgacatc
     2581 gtgccttcgc cggccgggat cagcgtaaac gacgccgctt cccgcgccat cgatacggtt
     2641 cttgccagtc ttgacgactc caaggccgaa aacatcgtct ccatcgacct tcaggggaaa
     2701 tcgagcctcg gtgactatat ggtcatcgca tcggggcgtt cgcatcgcca tgtcgcggcg
     2761 gtagcggatc atatcctcaa ggcgctcaag gatgcaggcc tcggtcaggc ccgcgtcgaa
     2821 gggttgcctg gcgccgactg ggtgctcatc gattcgggcg acgtcatcgt ccatgtcttc
     2881 cggccggaag tccgcgactt ctacaatctg gaaaagatgt ggcagacgcc ggatatcgag
     2941 gaggagacgc tgcactgaaa ggttgctgcg tcgccaaggg gcgtggatga agcttgtcgt
     3001 acatgccgtg ggccggctca aggctggccc cgagagagaa ttggctgacc gctatttcaa
     3061 gcgccttgcc aagagcgggc cgggcgtcgg catcgaattt tcagacatcg tggaaattcc
     3121 cgaagggcac gcccagacgg cgaacgagcg ccgccgcgag gaaggccagc ggctgcaaac
     3181 ccatatccag ccgggaacgg cgcttatcct gctcgacgag cgcggcaaga acctttcgtc
     3241 cgaggatttg gccggaaaga taggcctgct gcgcgatggc ggccgcaagg ccgtgctggt
     3301 ggcgatcggc ggcgccgacg ggcatgatgc gtctttgcac aagcaggccg aactggtttt
     3361 gtccttcggc gcgctgacat ggccgcatca actggttcgc gtgatgctgg ccgagcagct
     3421 ttaccgggtg gcgaccatcc tggccgggca tccctatcat cgctcctgac cttgaaaagc
     3481 aggaccggat tttcgcccca ttggcgcagg atatgctttc tctcgaccgg gcctggcgaa
     3541 catggttgat ggttcgttaa cgatgaagcc aatagagtcg ggcttgcatg tctgaaggca
     3601 gaaaatccat catcggcttt tggcgaggcg gcgcgtgcgc ggcagcggcg ttgatgcttt
     3661 tggcgcggcc ggcattggcg caggacagca ccgcgcaaac gcccgatccc gaccagaacc
     3721 gcgccgaata tgaaagcgtt tcccacgaaa tcaccctgtc gtcggaaagg ttggccaagc
     3781 tcgccgccga catcgcgacg gtaaaacaag atcatgcctc gatcaccgcg gcgctgatcc
     3841 agtcggcgaa gaccgaacag aagctcggcc aggatatcga ggatatcggc ggcaggctgg
     3901 aggggctgaa ggggcaggag gggaccatcc gcgcctcgct ggcaaagcgc cgcgacgtgc
     3961 tggccgaggt tctgggcgcg ttgcagcgca tggggatcaa tccgccgccg gccattctgg
     4021 tcaagcccga agacgcgctg tcgtcggtgc gcagcgccat cctgcttggc gcggtggttc
     4081 ccgaactgcg ggcgcagacc gacatactgg tttcgcagtt gaaggagctg acccgcgtga
     4141 ccgcttcgat cgaagcggag cgggcgcggc tgaccgcgac ggtgagcgag caggcggctg
     4201 aaaagcaacg gctgaccatg ctgctggaag ccaagcgcaa gcttcaggcc gacaccgaat
     4261 cggcgatggc cgaggagggc aggaaatccc aggatctggc ggccagggcg gcaagtctca
     4321 aagagttgat cgcctcgctg gagaaggacg ccgacaaaaa gcgcagcgcc aatgatgccg
     4381 cccgcgctgc cgccgaacag gcggcggcga atgccggcac ccaggccgag gccgatgccg
     4441 ccaaggcgca acttgcatcg ctggccgtgc cggaagccaa ccagcttgcg gcggcagcgc
     4501 cgttttcggc ccttcgggga cagatcgcgc tgccggtcat cggcaaggta aagcggcggt
     4561 tcggcgccga cgacggcaac ggcatggcca tgcttggcga catggttgcg acacaatccg
     4621 gctccatcgt gaccgcgcct gcggatggaa aagtgcttta tgcggggccg tttcgctctt
     4681 acggtcaact cttgatcctg gacgcgggcg acggctatca tgtcgtgctg gcgggaatga
     4741 ccagattaaa tgtcgcatcc ggccagtccg tgctcgcggg cgagccgatc ggcgcgatgg
     4801 gagaggccag ggtggcaagc gcctcggcct cgcttgatga aaacagtacg ccggaactct
     4861 atgtagagtt ccgcaaggat ggaaaacccg tcgatccggc cccgtggtgg gcggaccgtt
     4921 tctctggaag gacgtgaata tgcggaaatt gtcgctcttg tttgccggtg ccctgatggg
     4981 cgcgtcggcc atgagcctcg tctatggcgc gcccggctcg acggcaaacg cggctgcgtc
     5041 ggacacctac aagcagctgg cgatcttcgg cgatattttc gagcgggtgc gggcgcaata
     5101 tgtgacgccg cccgacgaca aggcgctgat cgagaacgcc atcaatggaa tgctgacctc
     5161 gctcgatccg cattcgtcct acatgaacgc ggaagccgcg caggacatgc gcgtgcagac
     5221 caagggtgag tttggcggcc tcggcatcga ggtcaccatg gagaacgacc tggtcaaggt
     5281 catcacgccg atcgacgaca cgccggccgc caaggcaggc gtgctggctg gcgactatat
     5341 cgccaagatc gacggcgaag aggttcgcgg cctgaccctc aacgacgcgg tcgagaagat
     5401 gcgcggcctg gtcaacacgc cgatcaagct caccatcctg cgccagggcg ccgacaagcc
     5461 gatcgagctg acggtgatgc gcgacatcat caaggtcaag gcggtcaagt tccgggtcga
     5521 gaacgacatc ggctacatga agatcacctc cttcaccgaa aagacctatg acgacctcga
     5581 gaatgcgatc gacaccatca agaagcaggt gccgaacgac aagctcaagg gctatgtgct
     5641 cgatctgcgc ctcaacccag gcggtctgct cgaccaggcg gtgagtgtgt cggatgcctt
     5701 cctcgaccgt ggcgagatcg tctcgacgcg cggccgcgat ccgaaggacg tcacccgctt
     5761 cgacgcccgg ccgggcgacg acatcaacgc caagccgctg atcgtgctcg tcaatggcgg
     5821 ctcggccagt gcatcggaaa tcgttg
//
LOCUS       DASYCS010000109         3083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3502403,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000109 DASYCS010000000
VERSION     DASYCS010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3502403"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..411)
                     /locus_tag="VGM46_03855"
     CDS             complement(16..411)
                     /locus_tag="VGM46_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019857758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HEY4191758.1"
                     /translation="MSDTDTTAESTALEATANAPAAVSAIPADELARLTDDVISALKT
                     VYDPEIPADIYELGLVYRIDIEDDRSVKIDMTLTAPGCPVAGEMPGWVENAVGAVEGV
                     SGVEVAMTFDPPWTPDRMSEEAQVAVGWY"
     gene            complement(417..1697)
                     /locus_tag="VGM46_03860"
     CDS             complement(417..1697)
                     /locus_tag="VGM46_03860"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEY4191759.1"
                     /translation="MVDFVDERAIDPRVKPEDNGRPYDVEAIRRDFPILSREVYGKPL
                     VYLDNGASAQKPQVVLDAIQHAYANEYANVHRGLHFLSNAATDAYEKARESVRRFLNA
                     PGTDNIVFTSNSTAAINTVAYGFGMPNIGAGDEIVLSILEHHSNIVPWHFIRERQGAK
                     LVWVPVDDLGAFHIEDFEKSLTDRTRLVAITHMSNALGTVTPIKEICRIAHERGIPVL
                     VDGSQSAVHMPIDVQDLDCDFFVFTGHKVYGPSGIGVLYGKKEMLERMRPFMGGGEMI
                     EEVTEDFVTYNEPPHRFEAGTPPIVQAIGLGAALDYMESVGRERIAAHEADLTHYAHE
                     RLRAINSLRIIGDAPAKGAIVSFELQGIHAHDVSMVIDRQGVAVRAGTHCAQPLLKRF
                     GVTSTCRASFGMYNTRAEVDALAGALEKARKFFG"
     gene            complement(1760..3025)
                     /gene="sufD"
                     /locus_tag="VGM46_03865"
     CDS             complement(1760..3025)
                     /gene="sufD"
                     /locus_tag="VGM46_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006205998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEY4191760.1"
                     /translation="MNMHLQPQRTQAETALIDAFGERQSLLPGDGAVMVKRDEAIELV
                     KHGLPTRRVEAWHYTDLRRLLATVPAYDDSAKAAALSPMVEGSPVLSLLNGVASKAPS
                     LANVTVSTVAEKLVDGSYAPALTAQGADDAIGALNAALVADGWFLDIADGAEFETPIE
                     LQNVQAGGQVHARLAVRIGSNVKATIVERQAGSGDALVSSVSNVVVGDDSELVWLIVQ
                     EEPDTATYLGQFNAWIGKNARLTLFVMNAGGKLVRQEIRVAAKGEGGEFTLRGINLLA
                     GDTHTDVTMVLDHEVPHTASTEIIRNVVTGRAHGVFQGRINVRQYAQKTDAKMACNTL
                     LLSDDGEFSTKPELEIFADDVVCGHGATVTEIDRNHLFYLMARGIDEKTARGLLVRAF
                     VAEVIEEMEDERIVEALEARLVDWFDTHG"
BASE COUNT          550 a          974 c          960 g          599 t
ORIGIN      
        1 cggaggcaag ggccgtcagt accagccgac ggcgacctgc gcttcttccg acatacggtc
       61 gggcgtccaa ggcgggtcga acgtcatggc aacttccacc ccggaaacgc cctcgacagc
      121 gcccacggcg ttttccaccc agccaggcat ttcgccggcc accgggcagc cgggcgcggt
      181 cagcgtcatg tcgatcttga cggagcggtc gtcctcgata tcgatgcggt agactaggcc
      241 gagttcgtag atatcggcgg ggatttcagg gtcgtagacg gtcttcagcg ccgaaatcac
      301 gtcgtcagtc agccgcgcca gttcgtcggc gggaatggcc gagacagcgg caggggcatt
      361 ggctgtcgct tccaatgcgg tggattcggc tgtggtgtct gtatcgctca tcatcgtcac
      421 ccgaagaact ttctcgcttt ttcaagcgcg ccggcgagag cgtcgacttc cgcccttgta
      481 ttatacatgc cgaacgatgc cctgcatgtg gaggttacgc cgaagcgttt caacagtggc
      541 tgggcgcaat gcgtgccggc gcgaactgcg acgccctgcc ggtcgatgac catggagacg
      601 tcatgcgcgt ggatgccctg aagttcgaac gaaacgatgg cgcccttggc cggcgcatcg
      661 ccgatgatgc gcagcgagtt gatggcgcgc aggcgttcat gcgcatagtg cgtcaaatcg
      721 gcctcgtgcg cggcgatgcg ctcgcgcccg acgctttcca tgtagtcaag cgctgcgccc
      781 agcccgattg cctggacgat aggcggcgtt ccggcctcga agcggtgcgg cggctcgtta
      841 taggtgacga agtcctccgt cacttcctcg atcatctcgc cgccgcccat gaaggggcgc
      901 atgcgttcca acatttcctt cttgccgtag agcacgccga tgccggacgg cccatagacc
      961 ttgtggccgg tgaagacgaa gaagtcgcaa tctaggtcct gaacgtcgat gggcatatgc
     1021 acggcgctct ggctaccatc gaccagcacc ggaatgccgc gctcatgggc gatgcggcaa
     1081 atttccttga tgggcgtgac cgtgcccagc gcgttcgaca tatgggtgat ggcgaccagc
     1141 ctggtgcggt cggtgaggct cttttcgaaa tcctcgatgt ggaacgcgcc gaggtcgtcc
     1201 accggcaccc acaccagttt cgcgccctgc cgttcgcgga tgaaatgcca cggcacgatg
     1261 ttggagtgat gctccaggat cgacagcacg atctcgtcgc ccgcgccgat attgggcatg
     1321 ccgaagccat aggcgacggt gttgatggcg gccgttgagt tcgaggtgaa gacgatgttg
     1381 tcggtgcccg gcgcattcag gaagcggcgc accgattcac gcgccttttc ataggcatcg
     1441 gtggcggcgt tcgacaggaa atgcagcccg cgatgaacgt tggcgtattc attggcgtag
     1501 gcgtgctgga tggcgtccag caccacttgc ggcttttgcg ccgacgcgcc gttatcgaga
     1561 tagaccagcg gcttgccata gacttcgcgc gacaggatgg ggaagtcgcg gcggattgcc
     1621 tcgacgtcat aggggcgacc gttatcctcg ggcttgaccc gaggatctat tgccctctca
     1681 tcgacaaaat cgaccatcgc ttcactccgg aacgcagcag atcctcggga caagcccgag
     1741 ggtgacgtca gtgctgatac tacccatgcg tgtcgaacca atccaccagg cgcgcttcca
     1801 gcgcctcgac gatgcgctcg tcttccattt cctcgatgac ttcggcgacg aacgccctga
     1861 ccagcaggcc gcgcgccgtc ttttcgtcga tgccgcgcgc catcaggtag aacaggtggt
     1921 tgcgatcgat ctcggtgacg gtcgcgccat gaccgcagac cacgtcgtcg gcgaagattt
     1981 ccagttccgg cttggtcgag aactcgccat cgtccgaaag cagcagcgtg ttgcaggcca
     2041 tcttggcgtc ggttttctgg gcgtactggc gcacattgat gcggccttgg aacacgccat
     2101 gcgccctgcc cgtgacgacg ttgcggatga tctcggtcga agccgtatgc ggcacttcat
     2161 ggtccagcac catggtcacg tccgtatgcg tgtcgccggc cagaaggttg atgccgcgca
     2221 gcgtaaactc gccgccctca ccctttgccg cgacgcggat ttcctggcgc accagcttgc
     2281 cgccggcatt catcacgaac agggtcagcc tggcgttctt gccgatccag gcgttgaact
     2341 ggccgagata ggtggcggtg tccggctctt cctgcacgat aagccagacc agctcggaat
     2401 cgtcaccgac gaccacattg ctgaccgacg aaacgagagc atcgccgctg cccgcctgcc
     2461 gttccacgat ggtggccttg acgttgctgc cgatacgcac cgccagccgg gcatggacct
     2521 gaccgccggc ctgaacgttc tgcaactcga tcggggtttc gaactccgcg ccatcggcga
     2581 tgtcgaggaa ccagccatcg gcgaccagcg ccgcattcag cgcgccgatt gcgtcgtcgg
     2641 cgccctgcgc cgtcaaagcg ggcgcatagc tgccgtcgac cagtttttca gcgacggtgg
     2701 aaaccgtgac attggccagc gacggcgcct tggaagcaac gccattgagc aaggacagca
     2761 ccggcgaacc ttcgaccatg gggctgagcg cggcggcctt tgcactgtcg tcataggccg
     2821 gcaccgtggc cagcaggcgg cgcaaatccg tatagtgcca cgcctcgacg cgtcgcgtcg
     2881 gcaagccatg cttgaccagt tcgatggcct cgtcacgctt gaccatcacc gcgccatcgc
     2941 ctggcaacag cgactggcgt tcgccaaaag cgtcgatgag cgccgtttcg gcctgcgtgc
     3001 gttggggttg caaatgcatg ttcataatct tccctcaggc ggcctcgccg atcacgccgg
     3061 catagccatt ggcctcaagc tca
//
LOCUS       DASYCS010000110         5123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3936681,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000110 DASYCS010000000
VERSION     DASYCS010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 5123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5123
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3936681"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..938)
                     /locus_tag="VGM46_03870"
     CDS             complement(48..938)
                     /locus_tag="VGM46_03870"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002963621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEY4191761.1"
                     /translation="MNLQSMTGFARSASELESSALVWEVKSVNGKSVDVRLRLPQGFE
                     RLEPAVRQTIQKRFLRGNFQAALTVSHAAGGYAQPVVNEAFLKDVAGLARRLQEQFDV
                     TPATADGLLALRGVLDIPERTESEETRASLDAAITATLEQALSGLVAARQAEGEALGA
                     LLSGQIDAIEALTLRAEADPSREPAAIRAKLAEQVRLLLDASANLDEGRLHMEAAFLA
                     TKADIREEIDRLKTHVASARGLLAAGGAVGRKLDFLAQEFNRESNTLCSKSNAATVTA
                     IGLELKAVVDQFREQVQNLE"
     gene            complement(958..2118)
                     /gene="mltG"
                     /locus_tag="VGM46_03875"
     CDS             complement(958..2118)
                     /gene="mltG"
                     /locus_tag="VGM46_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEY4191762.1"
                     /translation="MNTIPENNAEFGQQPAQSSPIIPKTASEALRPEAGTPPPKRSRA
                     SRSQVVVFMNFVISLIMLAVLAVGVAAYFGRQAFLEAGPSTAGDTFLVKPKMGVADIA
                     EQLERRNLISDARIFRIGVRAYGNDAALKAGEYEIKPLASMRDIMEILKSGKSVLYSL
                     TIPEGLTVEQALQRVSEQAALVGDMPATVPPEGSLATDTLRFTRGATRQEMVDKLLAD
                     QKKLVDEVWARRSPDLPLANIDEFVTLASIVEKETGKGDERSRVAAVFLNRLAKGMRL
                     QSDPTIIYGLFGGKGKPADRPIYQSDISKATPYNTYMINGLPPSPIANPGRAALEAVA
                     NPSKTKDLYFVADGTGGHVFAATLEEHNENVARYRKLKKQAEEADKTAEPAQ"
     gene            complement(2227..3489)
                     /gene="fabF"
                     /locus_tag="VGM46_03880"
     CDS             complement(2227..3489)
                     /gene="fabF"
                     /locus_tag="VGM46_03880"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019857269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEY4191763.1"
                     /translation="MRRVVVTGLGLVSPFGMGFEHGWKELLTGRSAAKRVTEFEVEDL
                     ACKVAHVIPRGDGSNGTFNPEAFLEPKELRKIGDFILYGIAAADEALKDSGWEMQSEA
                     DRYATGVMIGSGIGGLEGIAENAIILKERGPRRISPFFIPGNIINLVSGQVSIRHGLK
                     GPNHAVVTACSTGAHAIGDAARLIIFGDADVMLAGGAESPVTRLSLAGFAACRALSTE
                     RNDTPEAASRPYDRDRDGFVMGEGAGVVVLEELEHAKARGAKIYAEVTGYGLTGDAYH
                     ITAPAEDGDGAFRCMGAALKRAGLTAADIDYINAHGTSTMADTIELGAVERLVGDSAP
                     NISMSSTKSSIGHLLGAAGAAEAIFSILAIRDNIAPATINLDNPERETAIDLVANKPR
                     ARRIDVALSNSFGFGGTNASLVFQRFNG"
     gene            complement(3521..3757)
                     /locus_tag="VGM46_03885"
     CDS             complement(3521..3757)
                     /locus_tag="VGM46_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019170544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEY4191764.1"
                     /translation="MSDTAERVKKIVIEHLGVDVEKVTDQASFIDDLGADSLDTVELV
                     MAFEEEFGVEIPDDAAETILTVGDAVKFIDKASA"
     gene            complement(3943..4680)
                     /gene="fabG"
                     /locus_tag="VGM46_03890"
     CDS             complement(3943..4680)
                     /gene="fabG"
                     /locus_tag="VGM46_03890"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006216615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEY4191765.1"
                     /translation="MFDLTGRKALVTGASGGIGEAIARVLHAQGAIVGLHGTRVEKLE
                     ALASELGERVKLFPANLSDRDEVKALGQKVEADLEGVDILVNNAGITKDGLFVRMSDA
                     DWDSVIEVNLTAVFRLTRELTHPMMRRRHGRIINITSVVGVTGNPGQTNYAASKAGMI
                     GFSKSLAQEIATRNITVNCVAPGFIESAMTDKLNDKQKEAIMAAIPTRRMGTSAEVAS
                     AVAYLASNEAGYVTGQTLHVNGGMAMI"
     gene            complement(4734..>5123)
                     /locus_tag="VGM46_03895"
     CDS             complement(4734..>5123)
                     /locus_tag="VGM46_03895"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006331522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEY4191766.1"
                     /translation="CTEKGAKRALMLQVSAPFHSALMGPAAEAMREALATVQKHAPVV
                     PVVSNVTVTPTSDPDEIARRLVEQVTGRVRWRETVEWFGANGVTTLYEVGAGKVLSGL
                     ARRIDREIAVSAINGPADVEAALAALG"
BASE COUNT          929 a         1682 c         1503 g         1009 t
ORIGIN      
        1 aggccccggc gacgaatgcg cgaacccacg tctttggcaa ccatcggtta ctccagattc
       61 tggacttgtt cgcgaaactg atcgaccacc gccttcagtt ccagcccaat ggccgtcacc
      121 gtcgctgcat tcgatttcga acacagcgta ttcgattcgc gattgaattc ctgcgccaga
      181 aagtccagct ttcgtccgac agcgccgccg gcggccagaa gcccgcgtgc cgacgcgaca
      241 tgcgttttca gccgatcgat ttcttcacgg atatcggcct tggtggcaag gaaggccgcc
      301 tccatatgca gccgtccctc gtccagattg gccgaggcgt ccagaagcaa ccgcacctgc
      361 tcggcaagtt tcgcacgaat tgccgccggt tccctcgacg ggtcggcctc agcgcgcagc
      421 gtcaacgcct cgatggcgtc gatctgcccg gataacagcg ctcccagcgc ctcgccttcg
      481 gcctggcggg cagccaccag gccagacaat gcctgttcaa gcgttgctgt tatggcagcg
      541 tcgaggctgg cgcgcgtctc ttcggattcc gtccgctccg gaatatcgag caccccgcgc
      601 aacgccagaa gaccgtcagc ggtggcgggc gtaacgtcga actgttcctg gagccgccgg
      661 gcaaggccgg cgacgtcctt caaaaaggcc tcgttgacga ccggttgtgc ataaccgccg
      721 gctgcatggc tgactgtcag cgccgcctgg aaattgccgc gcaaaaatcg cttctgaatg
      781 gtctgccgca ccgccggttc aagccgctcg aagccctgcg gcaatcgcag gcgaacatcg
      841 acgctcttgc cgttgacgga tttcacttcc cagacaagcg cgctgctttc cagttccgag
      901 gccgaacggg caaaccccgt catgctctgc aaattcatag cgacctcgat accctgccta
      961 ttgcgccggt tcggcggtct tgtcggcttc ctcagcctgc ttcttcagct tcctgtagcg
     1021 agcgacgttt tcattgtgct cttcaagcgt cgcggcaaac acatggccac ccgtgccatc
     1081 ggcgacgaaa tacaaatcct tcgtcttcga cggattggcg accgcttcca gcgccgcccg
     1141 tccgggattg gcgataggcg acggcggcaa gccattgatc atataggtgt tatagggtgt
     1201 cgccttgctg atatccgact ggtagatggg ccggtcagcc ggctttccct tgccgccgaa
     1261 caggccgtag atgatcgtcg ggtcggattg cagccgcatg cccttggcca gccggttgag
     1321 gaaaaccgcc gcaacccgcg agcgttcatc ccccttgccc gtctccttct cgacgatgga
     1381 cgcaagcgtc acaaattcat cgatattggc cagcggcagg tcgggcgaac gccgcgccca
     1441 gacctcgtcc accagctttt tctggtccgc cagcagcttg tcgaccatct cctggcgcgt
     1501 cgcgccgcgc gtaaagcgaa gcgtgtccgt cgccagactg ccttccggcg gaactgtcgc
     1561 gggcatgtcg cccaccagcg ccgcctgctc ggacacacgc tgcaacgcct gctcgactgt
     1621 caacccttcc gggatggtca gcgaataaag caccgacttg ccgcttttca aaatttccat
     1681 gatgtcgcgc atggaagcca gcggcttgat ttcgtattcg ccggccttca atgccgcgtc
     1741 attgccgtag gcacgcacgc cgatgcggaa aatacgagcg tcgctgatca ggtttcgccg
     1801 ttcgagttgt tcggcaatat cggccacgcc cattttcggt tttacgagaa aggtgtcgcc
     1861 tgccgtcgat ggcccggctt cgagaaacgc ctgcctgccg aaataggcag cgacaccgac
     1921 cgccagaacc gccagcatga tcagcgagat cacgaaattc atgaacacca cgacctggct
     1981 gcgcgatgcg cgcgagcgtt tcggcggcgg cgtgcccgct tccggacgaa gcgcttcact
     2041 cgccgttttc gggatgatcg ggctggattg agccggctgc tgcccgaatt ctgcgttgtt
     2101 ctccgggata gtattcatga ctgcctatcg tttgactttg cggcgaatcc cctccggcac
     2161 agtaggggcg aatatggcaa aattgccaga ccggacaacc gctaaaaccg gcgaactact
     2221 gtccggtcag ccattgaaac gctggaagac cagcgatgcg ttggtgccgc cgaaaccgaa
     2281 ggaattggac aaggctacgt cgatccggcg cgcgcgtggc ttgtttgcca cgaggtcgat
     2341 cgccgtctcc cgctccgggt tgtcgagatt gatcgtggcc ggcgcgatat tgtcgcggat
     2401 ggccaggatc gagaaaatcg cttccgccgc gcccgccgcg cccagcagat ggccgatgga
     2461 cgacttggtc gacgacatcg agatattcgg agccgaatcg ccaaccaacc gctcgacagc
     2521 gccaagctcg atcgtgtcgg ccatggtcga ggtgccgtgg gcgttgatat agtcgatatc
     2581 cgcagccgtc agcccggccc gcttgagggc ggcccccatg cagcggaacg cgccgtcgcc
     2641 atcctcggcc ggcgcggtga tgtgataggc atcaccggtc agcccgtatc ccgtcacttc
     2701 cgcataaatc tttgcgccgc gtgccttggc atgttcgagt tcttcgagaa cgacgacacc
     2761 ggcgccctcg cccatgacga aaccgtcgcg gtcgcggtca taagggcgcg atgccgcttc
     2821 cggcgtatcg ttccgctcgg tcgaaagcgc ccggcaagcg gcaaagccgg ccagcgaaag
     2881 ccgcgtcacc ggtgattccg cgccgccggc cagcatcacg tcggcatcgc cgaagatgat
     2941 cagccgcgcc gcgtcgccga tagcgtgcgc gccggtcgag caagcggtga ccacggcgtg
     3001 attcggcccc ttcaatccgt ggcggatcga aacctggccg gaaacgagat tgatgatatt
     3061 gcccggaatg aagaacgggc tgatgcggcg tgggccgcgc tccttcagga tgatcgcatt
     3121 ctcggcaatc ccctcaaggc cgccgatacc cgagccgatc atcacgccgg tggcgtagcg
     3181 atcggcttcg gattgcattt cccagccgga atccttcagc gcttcgtcgg cggccgcgat
     3241 cccgtaaagg atgaagtcgc cgatcttgcg cagttccttg ggctcaagga atgcctcggg
     3301 attgaaggtg ccgttgctgc cgtcgccacg cggaatgacg tgcgcaacct tgcaggcaag
     3361 gtcttcaacc tcgaactcgg taacacgctt agctgcgctg cggccggtca gcaactcctt
     3421 ccagccgtgc tcgaagccca tgccgaaagg cgataccaga ccgaggcctg tgacgacgac
     3481 gcgcctcatc gcaggtctcc ctctggcaca gcgtaaagct tcaggccgaa gccttgtcga
     3541 tgaatttcac ggcatcgccg accgtcagga tcgtctcggc cgcgtcgtcg gggatctcga
     3601 cgccgaactc ttcctcgaac gccatgacga gttcgaccgt gtccaggctg tcggcgccca
     3661 gatcatcgat gaagctcgcc tggtcggtta ccttctcgac atcaacgccg agatgttcga
     3721 taacgatctt cttgacgcgc tctgcggtgt cactcatgtg ggcatcctcg tctattgttt
     3781 gtttatcttc gttagcgggc ggaatgccgc taatcaagct gaaagatggt cctgcctgaa
     3841 ggccggtccc tcaggtcgca tctcgcccgg tccgccgggt atgggccgaa tacacgagaa
     3901 tcggcccgcg tccaatgcga aatgggcgct tttccccatg cgtcaaatca ttgccatgcc
     3961 gccattgacg tgcagtgtct ggccggtgac atagcccgcc tcgtttgacg caaggtaagc
     4021 gaccgccgaa gccacttcgg cgctggtgcc catccggcgc gttggaatcg ccgccatgat
     4081 cgcctctttt tgcttgtcgt tgagcttgtc ggtcatcgcc gattcgataa atccgggcgc
     4141 cacgcaattg acggtgatgt tgcgggtggc gatttcctgc gccagcgact tggaaaagcc
     4201 gatcatgccg gccttggacg cggcgtaatt ggtctggccg ggattgccgg tgacgccgac
     4261 gaccgaggtg atgttgatga tgcggccatg gcggcggcgc atcatcggat gcgtcagttc
     4321 gcgggtaagc cggaaaacgg cggtcaggtt gacctcgatc accgaatccc agtccgcgtc
     4381 cgacatgcgc acgaaaagcc cgtccttggt gatgccggca ttgttgacca gtatgtcgac
     4441 gccttcgagg tcggcctcca ccttctggcc aagcgccttg acctcgtcgc gatccgacag
     4501 gttcgccgga aacagtttta cgcgctcgcc aagctcggat gccagcgcct caagcttttc
     4561 aacccgcgtg ccatgcagcc cgacaatcgc cccttgcgcg tgcagcacgc gcgctatcgc
     4621 ctcgcctatg cctccagatg cgccggtaac gagcgccttg cggccggtca gatcgaacat
     4681 tcttggctcc ttgagagaac acccataacg cggatgatgc cgtacttagt cgctcagccc
     4741 aaagctgcca atgccgcttc gacatcggcc gggccattga tggcgctgac ggcgatctcg
     4801 cggtcgatac ggcgcgccag gccggacagg accttgcccg ccccaacttc atagagcgta
     4861 gttaccccat tggcgccgaa ccattccacg gtttcgcgcc agcgcacgcg gcccgtcacc
     4921 tgctccacca gccgtcgcgc gatctcatcc gggtcgctcg tcggcgtcac ggtgacgttg
     4981 gaaaccaccg gcaccaccgg cgcatgcttt tgcaccgtgg cgagcgcctc gcgcatggcc
     5041 tcggccgccg gacccatcag cgccgaatgg aaaggcgccg aaacctgaag catcagcgcc
     5101 cgcttggcgc ccttttccgt gca
//
LOCUS       DASYCS010000111         4429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_894357,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000111 DASYCS010000000
VERSION     DASYCS010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4429
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_894357"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            248..535
                     /locus_tag="VGM46_03900"
     CDS             248..535
                     /locus_tag="VGM46_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007787860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HEY4191767.1"
                     /translation="MGHVDKRSISLSPELAAVVDDAVAAGEYASASEVVRDALRQWKD
                     RRDLFGYTLEELRALVQEGIDSGPARDGPPFLERLRARYSDMIADQGGLKG"
     gene            532..825
                     /locus_tag="VGM46_03905"
     CDS             532..825
                     /locus_tag="VGM46_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006336243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEY4191768.1"
                     /translation="MKYRLLPHAEADIEEIGDHISRQSPKAALRLIEAFAQRWELLAL
                     HPFSGIAREDIAADVRSIVVGEYLTFYRVGAEAIEILRVLHGRRDIDRDEIEG"
     gene            complement(831..1943)
                     /locus_tag="VGM46_03910"
     CDS             complement(831..1943)
                     /locus_tag="VGM46_03910"
                     /EC_number="4.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-lyase"
                     /protein_id="HEY4191769.1"
                     /translation="MSDAARRAAALPHLRNSGFAKGDPIPLPLTMASIFHTPGDATGV
                     NQYGRFGNPTWSAVEHMLEHLEGAPCVAFPSGMAAISSVFFSLLKAGDRVLLPSDGYP
                     ISRTVFERYLKGFGVEFDVRPTASYLDGGFEGYRLAFVETPSNPGLDICDIAAVAKAA
                     HAAGALLAVDNTTMTPLGQRPLDHGADIVVAADTKAPNGHSDVLFGHVASRNAEIISA
                     VQDWRKISGSIPSPFDAWMLHRGLETLEIRFDRMCTSAGLIARRLQAHPAVRSVRYPG
                     LESDPSHNLASAQMERFGFLISLVLESEGKAEDFINGCALMQAATSFGGVHTSAERRA
                     RSGDAVPPSFVRLSVGCEPTEELWKAIEESLDRVNS"
     gene            2357..2809
                     /gene="mraZ"
                     /locus_tag="VGM46_03915"
     CDS             2357..2809
                     /gene="mraZ"
                     /locus_tag="VGM46_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006204204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEY4191770.1"
                     /translation="MDRFLSSAVNRIDAKGRVSVPAHFRAVVQKRGYQELYALRGLDI
                     AAMDVGGLDLLDRYERRIALEDPFLQSSDDMSFFCHGDGAFLKLDQDGRITVTDFMRE
                     HTGIADEVAFVGRGDFFQMWEPGRLATYGAQVRARLLQLRQGTRERPE"
     gene            2806..3822
                     /gene="rsmH"
                     /locus_tag="VGM46_03920"
     CDS             2806..3822
                     /gene="rsmH"
                     /locus_tag="VGM46_03920"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEY4191771.1"
                     /translation="MTTGHGDEFHAVGGLARHIPVLLAEVLGALQPAGGQIIVDGTFG
                     AGGYTKAILAGGASVIAIDRDPDAIAAGRALEEQSGGRLRLVQAPFSTLDEHAEAIDG
                     VVLDIGVSSMQLDQAARGFSFRADGPLDMRMAQAGLSAADVVNNYKSGDLARIFGFLG
                     EERHAGRIARMIEARRQKHPFERTLELADAIETHIGRKPGDKIHPATRVFQALRIYVN
                     DELGELAKALFAAERALKPGGRLVVVTFHSLEDRIVKRFITDRADAASGSRYMPQVRA
                     RAATFEKSGGGVTAGDEETALNPRARSARLRAAIRTDAPARGGDFSIFGLPKLPGIDQ
                     PGER"
     gene            3829..4197
                     /locus_tag="VGM46_03925"
     CDS             3829..4197
                     /locus_tag="VGM46_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191772.1"
                     /translation="MFRTSDIVLIGVMVAAAAMTYTIKRNAELQMATVHKLQAQIHTE
                     EDTIDLLRADWSLLTQPRRLQKLTELYQPQLELAPVGGRQIVGLDQLPPRALSIENLT
                     AEEPGLPVDGQDPMATGGIQ"
     gene            4194..>4429
                     /locus_tag="VGM46_03930"
     CDS             4194..>4429
                     /locus_tag="VGM46_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013894664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEY4191773.1"
                     /translation="MIGKLLLRRGVKNSQAGSIVVDGARKATGGKAKTRVVMTMAVFF
                     GIYTMIAGRLVYLGYQDPDNSGAPASRVTASRPD"
BASE COUNT          840 a         1297 c         1486 g          806 t
ORIGIN      
        1 ccaagatgaa tggccgccac gtttccgctt cgcaaggagc gggggccata caaaacccgg
       61 cttataggcc agctgacgct ttattttccc ggaagaaatg tttgttttca gtctgttggc
      121 attggtggcg gacatattga atcaacaact tcgcaaaccg attcatttcc ggcttggggg
      181 tatggctggt ctgttgcaaa gcggtaggat taagtcgtac catctttcga agaacaggag
      241 caaagccatg ggtcatgtcg acaagcgcag catcagcctt tcgccggaac tggcggcggt
      301 ggtcgacgat gcggtcgccg ccggcgaata tgcatcggcg agcgaggtcg ttcgcgacgc
      361 ccttcgccag tggaaggacc ggcgcgacct gttcggctac acgctggaag agctgcgggc
      421 gctggtgcag gagggcatcg acagcgggcc ggcgcgagat ggcccgccgt tcctggaaag
      481 gttgcgtgcc agatattcag acatgatcgc cgatcagggc ggcctgaaag ggtgaaatat
      541 cggcttctcc cccatgcgga agccgatatc gaggagatcg gcgatcatat cagcaggcaa
      601 agtccgaaag ccgcgctcag gctgatcgag gctttcgcac aacgctggga gttactggct
      661 cttcacccct tctccggcat agcgcgggaa gacattgcgg cagatgttcg cagcatcgtt
      721 gttggcgaat atctgacttt ctaccgcgtt ggtgcagagg cgatagaaat tctccgcgtg
      781 ctgcatggcc ggcgcgatat cgaccgcgac gagatcgagg gctgagcgga tcagctgttg
      841 acccggtcca gcgattcctc gatggccttc cacaattcct cggtcggttc gcaaccgaca
      901 gacagccgca cgaagctcgg gggcacggca tcgccgctgc gtgcccggcg ctcggcggag
      961 gtgtggacgc cgccgaaaga ggtggcggcc tgcatcaggg cgcagccatt gatgaaatcc
     1021 tcggccttgc cttcggattc cagaacaagg ctgatgagga agccgaaacg ctccatctgc
     1081 gcgcttgcca gattgtgcga agggtcgctt tcgaggcccg gatagcgcac ggagcgcacg
     1141 gcgggatgcg cctgcaaacg ccgcgcaatc agcccggcgc tggtgcacat gcgatcgaag
     1201 cgaatttcga gcgtttccag gccgcgatgc agcatccagg catcgaacgg gctgggaatg
     1261 ctgccggaga ttttacgcca gtcctggacg gcggaaatga tctcggcatt gcggctggcg
     1321 acatggccga acagcacgtc tgaatggccg ttgggcgctt tggtgtcggc ggcaacgaca
     1381 atatcggcgc catggtccag cgggcgctga ccaagtggcg tcatagtcgt gttatccacg
     1441 gcaagcagcg cgccggcggc gtgggcggcc ttggcgacgg ctgcgatgtc gcaaatatcg
     1501 aggcccggat tggacggcgt ttcgacgaag gcgagacggt agccctcaaa gccgccgtcg
     1561 agataggagg cggtaggcct gacgtcgaat tcgacgccga accccttcaa atagcgctcg
     1621 aacacggtgc gcgatatcgg atagccatcg gaaggcaaaa gcacgcggtc gccggccttg
     1681 agcaggctga agaagaccga cgaaatggcc gccatgccgg agggaaacgc cacgcatggc
     1741 gcgccttcca gatgctccag catgtgctcg acggccgacc atgtcgggtt gccgaaacgc
     1801 ccgtactggt tgacgccagt ggcgtcgccc ggcgtgtgaa aaatggaagc catggtcagc
     1861 gggagcggaa tggggtctcc cttggcgaag ccgctattgc gcagatgggg aagggcggcg
     1921 gcgcgcctgg cggcatcgga catgaatgtg aacctgtggt tttcgagaac tggcgagcga
     1981 agccgccggc agacgctatt ccggccggcc tttgagggca agcatcgtcg gctgtgccag
     2041 gcaggtttta aacgcttcgt taggcttaat ggaggataaa cgagggaatg cccgtaaatc
     2101 cgtctgatgg cggttgggcg tggcatttat gaagctcgtt cccgttgacg cccatagtac
     2161 cccatgttat cccagatcga tattcaagcc atcggttcca gtcacggtaa agcgtacgcc
     2221 actcaggcca ccgctgcggc cgggcgtttg ccaatttccg acaagcagaa ccatggccgt
     2281 ttagagaccc ggacgctttg caaacaggcg agcggggaac tggagaaggg tgcggcttca
     2341 ggggctgcaa ggtgtcatgg accgttttct gtcgagcgcc gtgaaccgga tcgatgccaa
     2401 ggggcgggtt tccgtgccgg cgcatttccg cgcggtggtg cagaagcgcg gctatcagga
     2461 actctatgcg ctgcgcggcc tcgacattgc ggcgatggat gtcggcggtc tcgacctgct
     2521 cgaccgttac gagcggcgca tcgcgctgga ggaccccttt ctgcaatcga gcgacgacat
     2581 gtcgttcttc tgccatggcg atggggcgtt tttgaaactc gaccaggatg gccgaatcac
     2641 cgtgaccgat ttcatgcgcg aacacaccgg cattgccgac gaggtggcct ttgtggggcg
     2701 tggcgatttc tttcagatgt gggaaccggg ccggcttgcc acctatgggg cgcaggtgcg
     2761 ggcgcggcta ttgcagcttc ggcaggggac gagggagcga ccggaatgac gacaggccac
     2821 ggcgatgagt ttcacgccgt tggcggactg gcccgtcaca ttccggtcct tctcgccgaa
     2881 gttctgggcg cgttgcagcc ggcgggtggc cagattatcg tcgacggcac tttcggggcg
     2941 ggcggttaca ccaaggcgat ccttgccggc ggagcgtcgg tcatcgccat cgaccgcgat
     3001 cccgatgcga tagcagcggg acgggcgctg gaagaacagt cgggcggccg gttgcggctg
     3061 gtgcaggcgc cgttttcgac attggacgag cacgccgagg ccattgatgg cgtcgtgctc
     3121 gacatcggcg tgtcgtccat gcaactcgat caggcggcgc ggggcttttc ctttcgcgcc
     3181 gacggtccgc tggacatgcg catggcgcag gcgggcctga gtgccgccga tgtcgtcaat
     3241 aattacaagt ccggcgatct cgcccgcatc ttcggcttcc tgggcgaaga gcgtcatgcc
     3301 ggccgcatag cccgcatgat cgaggcgcgc cgccagaagc atccgttcga gcgcacactg
     3361 gaactggccg acgcgatcga aacgcatatc ggccgcaagc cgggcgataa aatccacccg
     3421 gcgacgcgcg ttttccaggc cttgcgcatc tatgtcaatg acgagctggg cgaactggcc
     3481 aaggcgcttt tcgcggccga acgcgcgctg aagcccggcg ggcggctggt ggtggtgacg
     3541 ttccattcgc tggaagatcg catcgtcaag cgcttcatca ccgaccgcgc ggatgctgct
     3601 tccggctcgc gctacatgcc gcaggtgcgg gcgcgggcgg cgacgtttga gaagtcgggc
     3661 ggcggcgtta cggccggcga tgaggaaacc gcgctcaatc cacgggcgcg ctcggccagg
     3721 cttcgcgcgg ccatccgcac cgacgcgccg gcgcgcggcg gcgatttttc cattttcggc
     3781 cttccaaagc ttcccggcat cgaccagccc ggcgagaggt gagcgttcat gtttcgaacc
     3841 agtgacattg tgctgatcgg cgtcatggtc gccgccgccg ccatgaccta cacgatcaag
     3901 cgcaacgccg aattgcagat ggcgaccgtc cataaattgc aggcgcaaat ccacaccgag
     3961 gaagacacta tcgacctgtt gagggccgac tggagcctgc ttacgcagcc gcgacggcta
     4021 cagaaactga ccgaactcta tcagccccag ctggagcttg cgccggtcgg cggccgccag
     4081 atcgtcggtc tggaccagtt gccgccccgc gcgctcagca tcgagaactt gactgccgaa
     4141 gaacccggcc tgccggtgga cgggcaagac ccgatggcca cgggaggcat ccaatgatcg
     4201 gcaagttgct gctgcggcgc ggcgtaaaga acagccaggc cggctcgatt gtcgtggacg
     4261 gcgcccgcaa ggcgaccggc ggcaaggcca agacgcgcgt agtgatgacg atggccgtgt
     4321 ttttcggtat ctacacgatg attgccggcc ggctggtcta tctcggctat caggacccgg
     4381 acaattccgg cgcgccggcc agccgggtga cggcatcgcg gcccgatat
//
LOCUS       DASYCS010000112        11746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2922875,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000112 DASYCS010000000
VERSION     DASYCS010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 11746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11746
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2922875"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..1170
                     /gene="hisC"
                     /locus_tag="VGM46_03935"
     CDS             61..1170
                     /gene="hisC"
                     /locus_tag="VGM46_03935"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEY4191774.1"
                     /translation="MTTAQDRQRPQPRAGIMDIEAYVPGKSTAPAGVAKVHKLSSNEN
                     PLGASPKAIEAAREAAGRLETYPDGSARRLREALGEAHGLDPANIVCANGSDEILGLL
                     SQTYLSPGDEAIFTEHAFLVYKIYVQASGATPVCVKETDERADIDAMLAAVTGRTGIV
                     FLANPNNPTGTYLPFEEVKRLHAGLPKGVLLVLDAAYAEYVRRDDYQAGVEMVSSSQN
                     VVMVRTFSKLGLGGLRLGWMYAPTHVCEAINRVRGPFNVNTVAIEAGIASVRDHAHVE
                     RSVAHNETWLAWLDGELKQLGLRVTPSVGNFLLVHFPDDGKHSAAAADDYLSARGYIL
                     RRVTAYGFPNALRMSVGTEEGNRGAIAALTEFLKS"
     gene            1175..2110
                     /locus_tag="VGM46_03940"
     CDS             1175..2110
                     /locus_tag="VGM46_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate/arogenate dehydrogenase family
                     protein"
                     /protein_id="HEY4191775.1"
                     /translation="MPEPLFEKIALVGIGLIGSSLARVIAREKLAGHVAISTRSADTL
                     ARAEELRLGDSYTTDAREAVRDADLVVVSVPVGSSGAVAQEIAPALKKGAILTDVGST
                     KASVIAQMQPFVPQGVHFIPGHPLAGTEKSGPDAGFAELFENRWCIFTPLPDTDPAAL
                     EKLSEFWRRCGANIDTMDPAHHDMTLAIVSHLPHIIAYNIVGTADDLESVTKSEVIKY
                     SASGFRDFTRLAASDPTMWRDVCLHNKEAILEMLARFSEDLSSLQRAIRWGDGDKLFE
                     LFTRTRAVRRSIIEAGQDVDTPDFGRQGMEHPASK"
     gene            complement(2128..3126)
                     /locus_tag="VGM46_03945"
     CDS             complement(2128..3126)
                     /locus_tag="VGM46_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2125 domain-containing protein"
                     /protein_id="HEY4191776.1"
                     /translation="MTASDQPKPKKRSRLFVWLAILVALFAAYSAGWYALANKVRGEV
                     GAAIAAINAGGANADCANMSVSGYPLRLVVSCDNLAYENDTTNVAASTGRMDAMARIY
                     RPLSPVADIVGPLRTSAPGMAPLWLDWDKMRAAASLSLTSTAGLALRGEGVSGQTDPQ
                     DDSEPVQLFSAGKAEVMLQPVGQDVDAGFSFSDLQVDAEALDGRVLPPLNGAGAATLK
                     NGLALLGAKAETLRGQSIDIGQLELSSGTAHISVSGPIEVDADGLIDASLTIRLQDPK
                     AAAAILGAAIPEQKRQIEQGFSALAMFGSEPTMPLKITKGKASLGFIPLGDIKPVQ"
     gene            3254..3775
                     /locus_tag="VGM46_03950"
     CDS             3254..3775
                     /locus_tag="VGM46_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HEY4191777.1"
                     /translation="MGDFWVFGYGSLMWRPGFAHTDTQRARLFGFRRALCVYSFVHRG
                     TPERPGLVLGLDRGGSCIGMAFRVTGSLRDEVIGYLRAREQVTGVYLERRLPLRLENG
                     EAVEAVAYIADRGHEQYAGALDAAHAAAVVTGAAGMSGPNEAYVLSTIEHLQALGIRD
                     RWLEDVAGRITAV"
     gene            3893..4840
                     /locus_tag="VGM46_03955"
     CDS             3893..4840
                     /locus_tag="VGM46_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY4191778.1"
                     /translation="MQKRRLGRTDLTIAPLVFGGNVFGWTVDEKTSFSLLDRFFEAGL
                     NAIDTADVYSRWAPGNSGGESETIIGKWMKERGNRDRLVIVTKVGSDIGQGKIDLSAP
                     HIEKAIDASLKRLQTDVVDLYLSHWPDPTVPHEETLGAYQKLIAKGKVRFIGASNLDA
                     GQMRAALDVARLRDLPRYEVLQPGYNLYDRDGFDGPLRDLCMNEDLGVICYYGLAKGF
                     LSGKYRSKADLGKSPRGDGVQEYLNPRGMRILAALDAVSARHSATQAEVALGWLIARP
                     GITAPIASATRLEQVDSLIKAVSLKLGADDIAELDRASA"
     gene            complement(4892..5686)
                     /locus_tag="VGM46_03960"
     CDS             complement(4892..5686)
                     /locus_tag="VGM46_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006200220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEY4191779.1"
                     /translation="MLQIRSLAFNFVFYVSLIVQMVLWAPFYFLSPRHRAWFVPKFWA
                     RTSLWLHEKIAGTRSEITGQENLPEGSFILAPKHQSFWDAIAFFPYLRDPLYILKREL
                     TWIPFFGWYIMKMRMIPVDRGSRSKALKMVVAATREELARNPRQLIIYPEGTRRPPGA
                     EPAYKYGIVELYNQLGVPVVPVAHVAGLFWPRRKFLRYPGTIHARFLPAIPPGLGKEE
                     FMARLIGETEAACDRLLVETANASDAPHLPPSALRRLEELRAAGKA"
     gene            complement(5777..6484)
                     /locus_tag="VGM46_03965"
     CDS             complement(5777..6484)
                     /locus_tag="VGM46_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEY4191780.1"
                     /translation="MNWQDSIESSARARAAAARKPGPTRLRLAVRVCAILFAGAAALF
                     AGGFGWFANHVAHLATPANPEKADAIIVLTGGASRLDAAMELLEVGKGQRLLISGVHP
                     SATRRQLQIATGGDKQLFSCCVDIDHAALDTIGNAEESAKWVGDHAYGSVILVTNNYH
                     MPRTLLEMGRLLHNARLEPYPVVNSNLDNGGWLTKPEALRVLFTEYNKYVLALARGIL
                     PVQPVTTTVSVAEVTAR"
     gene            complement(6576..7571)
                     /locus_tag="VGM46_03970"
     CDS             complement(6576..7571)
                     /locus_tag="VGM46_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4191781.1"
                     /translation="MTDTFADDPGQDEILEGGLDQAPRRQRRMASIVPAQNIAGRALV
                     LVIAIMTFLSCLTFGAVTLVRDTASVWENQISREATIQIKPVDGLDMEAALAAAQELA
                     GGFPGVTGASIVDRAATARLLEPWLGAGLDIDELPVPRLVVVTIDEQNPPDFAAMRKA
                     LVEKVASATLDDHRTWVDRLVAMAHTTVTIGIAVLTLMLSATVLTVVFATRGAMAGNG
                     HIIEVLHFVGAEAGFIAREFRRHFLLTGMKGAAAGGVAAIVVFVIFYWWSSHNMATPQ
                     ADQATALFGNFAIGYAGYLGVVLMVLVIGALTAATSHATVVAYLSDIEVRQHDPA"
     gene            complement(7564..8223)
                     /gene="ftsE"
                     /locus_tag="VGM46_03975"
     CDS             complement(7564..8223)
                     /gene="ftsE"
                     /locus_tag="VGM46_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002965062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEY4191782.1"
                     /translation="MIRFENVGLRYGMGPEILRDISLHIPERSFQFLSGPSGAGKTTL
                     LRLLFMSLKPTRGLITMFGKDRARISRTELPLLRRRIGVVFQDFRLLDHMTTYENVAL
                     PLRVRGRDEASYRTDVIELLKWVGLGERMHVLPPVLSGGEKQRAAIARALIEQPEILL
                     ADEPTGNVDPPLARRLLRLFIELNRLGTAVVIATHDLGLMEQVDARRMILAGGRLDVY
                     D"
     gene            8415..9068
                     /locus_tag="VGM46_03980"
     CDS             8415..9068
                     /locus_tag="VGM46_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191783.1"
                     /translation="MADARTARPVSGEIMTDAASGRAFGRAAPAIAPDIIDADYEVLS
                     HGEERVPDVQPAAQPRMVDAPAVEGMDMLRKPDGAPAPARVSRGGPIFWVAGLGAALA
                     AFWVSGGDALVRHAPLFAVRDAAYSAFSISGVKSHVDLSRQNPLLFIDGEASNDGNGA
                     SALPPLEIRVTGNDGRITRYTLGTAGRPLAPGERFAFSSRLDVPKNGVRTVSVAFAE"
     gene            9093..9635
                     /gene="hpt"
                     /locus_tag="VGM46_03985"
     CDS             9093..9635
                     /gene="hpt"
                     /locus_tag="VGM46_03985"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEY4191784.1"
                     /translation="MPVVRGKDIEVLFSASAIARRNLELAKEIAGNDYHDLLVISILK
                     GSFIFAADLIRAMHDVDLSPEMEFIFISSYGAGTASGEVRVLRDIDNEVAGRDILLID
                     DILESGKTLTFTRDLMLSRGAKSCSIAVLLDKRMRRQTDLNADYVGFDCPDHFVVGYG
                     MDAGHAFRELPFVGIVRGEA"
     gene            9677..10048
                     /locus_tag="VGM46_03990"
     CDS             9677..10048
                     /locus_tag="VGM46_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY4191785.1"
                     /translation="MAKLLIVEDDASVRILCARAFERAGHSVDVAEDGAQGLARIVEA
                     RGGYSLVVSDIRMPEMDGIEMAKAAADAFPSLKILLMTGYADQRERAEDLNGIIVDVV
                     QKPFTLAEIRARVDQALACFA"
     gene            complement(10088..10618)
                     /gene="ppa"
                     /locus_tag="VGM46_03995"
     CDS             complement(10088..10618)
                     /gene="ppa"
                     /locus_tag="VGM46_03995"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEY4191786.1"
                     /translation="MRIEAIATGSNPPEDVNVIIEVAVGGEPIKYEMDKDAGTLVVDR
                     FLYTPMRYPGNYGFVPHTLSGDGDPIDVLVCNTRALVPGCVINVRPIGVLLMEDNAGM
                     DEKVIAVPSSHITKRYEKVANYTDLPQITLDQVAHFFEHYKDLEPGKWVKLGGWRDAA
                     YAKKMIVEAVERAKGK"
     gene            complement(10746..11252)
                     /locus_tag="VGM46_04000"
     CDS             complement(10746..11252)
                     /locus_tag="VGM46_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006327367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY4191787.1"
                     /translation="MNTLTIDIRRAEPRDAAAIADVHQQAWHGAYAGIIPHRALTSMI
                     NRRGSDWWANAIRRAATVLVVEIGGKIAGYTTLGKNRARELRQQGEIYELYLRPEYQG
                     IGLGSRLFAAARARLADNGLKGMVVWALEDNENALSFYASAGGRDVAEGVEIFEQKAL
                     KKVAFVWE"
     gene            11526..>11746
                     /locus_tag="VGM46_04005"
     CDS             11526..>11746
                     /locus_tag="VGM46_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEY4191788.1"
                     /translation="MNPHPRDVAIPNDWDRRGLPGWSYHSDALLDLEKEHVFRNHWQI
                     AGHVSDVPNAGDYLTMDVVGERALIVRGK"
BASE COUNT         2099 a         3600 c         3919 g         2128 t
ORIGIN      
        1 gccggtcgtg gcttgtattt ccgcagcgtc ttctggattt tgaagcatgg aaccggcacg
       61 atgaccacgg cacaggatcg gcaacgcccg caaccgcgcg cgggcatcat ggacatcgaa
      121 gcctatgtgc cgggcaagag cacggcacct gccggcgtcg ccaaggttca caagctgtcg
      181 tcgaacgaaa acccgcttgg ggcgtcgccc aaggcaatcg aggcggcgcg cgaggctgcc
      241 gggcggcttg aaacctaccc agacggttcg gcgcggcgtc tgcgcgaggc gcttggcgag
      301 gcgcacgggc tggacccggc aaacatcgtc tgcgcgaacg gttccgatga aatactgggc
      361 ctgctgtcgc aaacctatct gtcgcccggc gatgaggcga tcttcaccga gcacgccttc
      421 ctagtctaca agatttacgt tcaggcatcg ggggcaacgc ccgtctgcgt caaggaaacc
      481 gacgagcgcg ccgatataga cgccatgctg gcggcggtta ccggcaggac cggcatcgta
      541 ttcctggcca acccgaacaa tccgacaggc acctatctgc cgttcgaaga ggtgaagcgg
      601 ctgcatgccg gtctgcccaa gggcgtgctt ctggtgctgg atgcggccta tgcggaatat
      661 gtgcggcgcg acgattacca agcgggcgtg gagatggttt cgtcctcgca aaatgtcgtc
      721 atggtgcgca ctttctccaa gcttggcctc ggcgggttgc ggcttggctg gatgtatgcg
      781 ccgacgcatg tgtgcgaggc gatcaaccgc gttcgcgggc cgttcaacgt caatacggtg
      841 gccatcgagg cgggcatcgc ctcggtgcgc gaccacgccc atgtcgagcg cagcgtcgcc
      901 cataacgaga cctggctggc ctggctcgac ggcgaactga agcagctcgg cctgcgggtg
      961 acgcccagcg tcggcaattt cctgcttgtc cactttcccg acgatggcaa acattcggcg
     1021 gcggcggcgg acgattatct gtcggcgcgc ggctatatat tgcggcgcgt caccgcctac
     1081 ggttttccaa acgcgctgcg catgagcgtc ggcaccgagg aaggcaatcg cggcgccatc
     1141 gccgcactca cggaatttct gaaaagctga agccatgccc gaaccgctgt ttgaaaagat
     1201 cgccctcgtc ggcatcggcc tgatcgggtc ttcgctggcg cgcgtcattg cccgcgagaa
     1261 gcttgccggc catgtcgcca tttcgacgcg cagcgccgac acgctggcgc gggccgagga
     1321 actgcggctt ggcgacagct atacgaccga tgccagagag gccgtgcgcg acgccgacct
     1381 cgtcgtcgta tcggtgccgg tcggctcgtc cggcgcggtg gcgcaggaga tcgcgccggc
     1441 gctgaagaag ggcgcgatcc tcaccgatgt cggctcgacc aaggcttcgg tcatcgcgca
     1501 gatgcagccc ttcgtgccgc agggcgtgca tttcattccg ggccatccgc tggccggcac
     1561 ggaaaaatca ggcccggatg cgggctttgc cgaactgttc gaaaaccgct ggtgcatctt
     1621 cacgccgctg cccgataccg acccggctgc gttggaaaaa ctgtcggagt tctggcggcg
     1681 ctgcggggcc aatatcgaca cgatggaccc tgcccaccac gacatgacgc tggcgatcgt
     1741 ctcgcacctg ccgcacatca tcgcctacaa catcgtcggc acggcggacg acctggaatc
     1801 ggtgaccaag tcggaagtca tcaaatattc cgcctccggt ttccgcgact tcacgcgcct
     1861 tgcggcctcc gacccgacca tgtggcgcga cgtctgcctg cacaacaagg aggcgatcct
     1921 tgaaatgctg gcgcggtttt ccgaagacct ttcttcgttg cagcgggcga tacgctgggg
     1981 cgacggcgac aagctgttcg agctgttcac ccgcacccgc gcggtgcgcc gctcgatcat
     2041 cgaggccggg caggatgtcg atacgcccga tttcggccgg caagggatgg agcatccagc
     2101 cagcaaatag gctggatgcc gccgccgtta ctggacgggc ttgatgtcgc ccagtgggat
     2161 gaagccgagc gaggcttttc ccttggtgat cttcaggggc atggtcggtt cgctgccgaa
     2221 catggccagc gcggagaagc cctgctcgat ctggcgcttc tgctcgggga tggcggcgcc
     2281 caaaatggcg gctgccgctt tcgggtcctg gaggcggatg gtcaggctgg cgtcgatgag
     2341 gccgtcggca tcgacttcta tcggcccgga cactgaaata tgcgcggtcc ccgacgacag
     2401 ttcgagttgc ccgatgtcga tcgattggcc gcgcagggtt tcggccttgg cgccaagcag
     2461 cgccagcccg ttcttcaagg tggcggcgcc ggcgccgttc agcggcggca gcacgcgtcc
     2521 atcgagcgcc tcggcgtcga cttgcaggtc gctgaagctg aagccggcgt ccacatcctg
     2581 gccaacgggt tgcagcatga cctcggcctt gccggcgctg aacaattgca ccggttcgct
     2641 gtcgtcctgc ggatcggtct ggccggacac gccctcaccc cgcagcgcca gtcccgccgt
     2701 cgaggtcagc gaaaggctgg ctgcggcgcg catcttgtcc cagtcgagcc agagcggggc
     2761 catgccgggc gcggaggtgc gcaacggtcc gacaatgtcg gcaaccggcg acagcggccg
     2821 atagatgcgc gccatcgcgt ccatcctgcc ggtcgaggcg gcgacattgg tggtgtcgtt
     2881 ctcataggcg aggttgtcgc aggagacgac gaggcgaagc ggatagccgc tgacgctcat
     2941 attggcgcaa tcggcattgg cccccccggc gttgatggcg gcgatggccg cgccgacttc
     3001 gccgcgcacc ttgttggcaa gggcgtacca gccggcgctg taggcggcaa aaagcgcgac
     3061 gaggatggcg agccacacaa aaaggcggga acgctttttc ggcttcggct ggtcgctggc
     3121 tgtcatggtc gggctttcgc tttagtggat ggctggaaaa acgtttccgg gcggggtcta
     3181 tggttgggtt gaaccggctg gacaaagcat tgttttggcg cgacacccga cgcgatgcaa
     3241 ccaggctagc gatatgggcg atttttgggt ttttggctat ggctcgctga tgtggcggcc
     3301 gggctttgcg cataccgata cgcagcgggc gcggctgttc ggctttcgcc gggcgctatg
     3361 cgtctactcc ttcgtgcatc gcggcacgcc cgaaaggccg ggcctggtgc tcggtctcga
     3421 ccggggcggc tcctgcatcg gcatggcgtt tcgcgtgacg ggttctttac gcgacgaggt
     3481 catcggctat ctgcgggcgc gcgagcaggt caccggcgtc tatctggagc gcaggctgcc
     3541 gctgcggctg gaaaacggag aggccgtcga ggcggtggcc tacatcgccg accgcgggca
     3601 tgagcaatat gccggcgcgc tcgatgccgc gcatgcggcg gcggtggtga ccggcgctgc
     3661 cggcatgtcc ggccccaacg aggcctatgt gctcagcacg atcgagcatt tgcaggcgct
     3721 cggcattcgc gaccgctggc tggaggacgt cgccggccgg atcacggctg tttgaacgac
     3781 gcatcgccgc acaggctgtt aaaccgggcc gggcttgcgg attcggctgc ggccggacct
     3841 ggatttccac ccgccagcac ctggcttacc gacccttgaa cggagaaata ccttgcaaaa
     3901 acgccgcctc ggtcgaaccg acctcaccat cgcgccgctg gttttcggcg gcaatgtctt
     3961 cggctggacg gtggacgaga aaacctcgtt cagcctgctc gaccgcttct tcgaggcggg
     4021 cctgaacgcc atcgacaccg ccgatgtcta ttcgcgctgg gcaccgggca attcgggcgg
     4081 cgaatccgaa accatcatcg gcaagtggat gaaggagcgc ggcaaccgcg accggctcgt
     4141 gatcgtcacc aaggtcggtt cggatatcgg ccagggcaag atagatttgt cggcgccgca
     4201 catagaaaaa gccatcgatg cttccttgaa gcggctgcaa accgatgtgg tcgatcttta
     4261 tctgtcgcat tggcccgacc cgaccgtgcc gcatgaggag acgctgggcg cgtaccagaa
     4321 gctgatcgcc aaggggaagg tgcgcttcat cggcgcgtcc aacctcgatg ccggccagat
     4381 gcgggcggcg ctcgacgtgg ccaggctgcg cgacctgccg cgctacgagg ttttgcagcc
     4441 gggctataat ctctacgacc gcgatgggtt cgacggaccg ctgcgcgatc tgtgcatgaa
     4501 cgaagatctc ggcgtgatct gctattacgg gctggccaag ggctttctct ccggcaaata
     4561 ccgcagcaag gccgatctgg gcaaaagccc gcgcggcgat ggcgtgcagg aatatctcaa
     4621 cccgcgcggc atgcgcattc tggctgcgct cgacgccgtg tcggcgcggc acagcgcgac
     4681 gcaggcggag gtggcgcttg gctggctgat cgcacggccg ggcattaccg cgccgatcgc
     4741 cagcgcgacc cggctggaac aagtcgacag cctcatcaag gcggtttcct tgaaactggg
     4801 agccgacgac atcgccgaac tcgacagggc gagcgcttaa gcctgggctg gggtcaagcg
     4861 gggccggaaa gcggccctgc cggcctgaac cttacgcctt gccggcggcg cgcagttctt
     4921 ccagccgcct gagcgccgaa ggcggcagat gcggcgcgtc gctggcgttg gcggtttcga
     4981 ccagcagccg gtcgcaggcc gcttccgtct cgccgatcag ccgggccatg aattcctcct
     5041 tgccaaggcc cggcggtatg gcgggcagga agcgcgcatg gatggtgccg ggatagcgca
     5101 ggaatttccg gcgcggccaa aacaggccgg cgacatgcgc caccggcacc accggcacgc
     5161 ccagctggtt ataaagctcg acgatgccgt atttataggc cggctccgcg ccgggcgggc
     5221 ggcgggtgcc ttcgggatag atgataagct ggcgcgggtt gcgcgccagt tcttcccttg
     5281 tcgcggcaac caccatcttg agcgccttcg agcggctgcc ccggtcgacc gggatcatgc
     5341 gcatcttcat gatgtaccag ccgaagaacg gtatccaggt cagttcgcgc ttgaggatgt
     5401 agagcgggtc gcgcagatag gggaagaagg cgatcgcgtc ccagaaggac tggtgctttg
     5461 gcgcaaggat gaacgagcct tcgggcaggt tttcctgccc ggtgatttcg ctgcgcgtgc
     5521 cggcgatttt ttcgtgaagc caaagcgagg ttcgcgccca gaatttcggc acgaaccagg
     5581 ctcggtgacg cggcgacagg aaatagaacg gcgcccacag caccatctgc acgatcaggc
     5641 tgacatagaa gacgaagttg aaggccagcg aacggatttg cagcatggag gaaaggcccg
     5701 gtgaggatta ttggcgctgg ttacaccaag gccgcgcaaa aagaaaacag cagaccggct
     5761 gcggcagcgt cgagcgctag cgagcggtaa cttcggccac cgaaacggtc gtcgtaaccg
     5821 gctgcaccgg caggatgcca cgggccagcg ccagcacgta tttgttgtat tcggtaaaca
     5881 gcacacgcag cgcctcgggc ttggtcagcc agccgccatt gtcgaggttg gaattgacca
     5941 ccgggtacgg ttcaagcctg gcattgtgca gcaggcggcc catttccagc agggtgcgcg
     6001 gcatatggta gttgttggtg acgaggatga cgctgccata ggcgtggtcg cccacccatt
     6061 tggcgctctc ctcggcattg ccgatggtgt cgagcgcggc atggtcgata tcgacgcagc
     6121 aggagaacag ttgcttgtcg ccgccggtgg ctatctgcaa ttgccgccgc gtggcggaag
     6181 ggtggacgcc gctgatgaga agccgctggc ccttgccgac ctccagcagt tccatcgccg
     6241 cgtcgaggcg cgaggcgccg ccagtcagga cgatgatggc gtcggccttt tccgggttgg
     6301 cgggcgtcgc cagatgggcg acatggttgg cgaaccagcc gaaaccgccg gcaaacagcg
     6361 cggctgcgcc cgcaaacagg atggcgcaga cgcgtacggc cagtcgcagg cgggtgggtc
     6421 ccggcttgcg tgccgccgcg gcccgcgccc gggcgctcga ttcaatcgaa tcctgccagt
     6481 tcatgttgtc atggttaacc ctgaacgcag gccgttggta gttaaattgc acactttcgg
     6541 tgacgccagg atgcgtgtcg tgatgagaat tgcggtcagg ccgggtcatg ctggcgcacc
     6601 tcgatatcgc tgagataggc gaccaccgtc gcatgggagg tcgcggcggt cagcgcgccg
     6661 atgaccagca ccatcaggac cacgccgaga tagccggcat agccgatggc gaaattgccg
     6721 aacagggcgg tggcctgatc cgcctggggc gtggccatgt tgtgcgacga ccaccagtag
     6781 aaaatgacga acacgacgat ggcggcgacg ccgccggcgg ccgcgccctt catgccggta
     6841 agcaggaaat gccgccggaa ttcgcgtgcg atgaagcccg cttccgcgcc gacgaaatgc
     6901 agcacctcga tgatgtgccc gttgccggcc atggccccgc gcgtggcgaa caccaccgtc
     6961 aaaacggtcg ccgacagcat cagcgtcagc acggctatgc cgatggtgac ggtcgtgtgc
     7021 gccatggcga cgaggcggtc cacccaggtg cggtggtcgt ccagcgtggc gctggcgact
     7081 ttctcgacga gggccttgcg catggcggcg aagtcgggcg ggttttgctc gtctatggtg
     7141 acgacgacga ggcggggcac cggcagttca tcgatgtcca gccccgcgcc cagccacggt
     7201 tccagcaggc gcgccgtcgc cgcgcggtcg acgatgctgg cgccggtgac gccgggaaag
     7261 ccgccggcaa gttcctgcgc agcggcaagg gccgcctcca tatcgaggcc atcgaccggc
     7321 ttgatctgga tggtggcttc ccgcgagatc tggttttccc agaccgatgc ggtgtcgcgc
     7381 accagcgtga cggcgccgaa tgtcaggcac gacaggaacg tcatgatggc gatgaccagc
     7441 accagcgcgc ggccggcgat gttctgcgcc ggcacgatgg acgccatgcg gcgctggcgc
     7501 cggggagcct gatcgaggcc gccttcgagg atttcgtcct gtccggggtc gtccgcgaag
     7561 gtgtcagtca tagacgtcca gccttccgcc ggccagaatc atgcgccgcg catcgacctg
     7621 ctccatcagg ccgaggtcgt gggtggcgat gacgacggcc gtgcccagcc ggttaagctc
     7681 gatgaacagg cgcagcaggc gacgcgccag cggcggatcg acattgccgg tcggctcgtc
     7741 ggccagcagg atttcgggct gctcgatcag ggcgcgggca attgcggcgc gctgcttctc
     7801 gccgcctgaa agaaccggcg gcagcacatg catgcgctcg cccagcccga cccatttcag
     7861 caattcgatg acatcggtgc ggtagctggc ctcgtcgcgc ccgcgtacgc gcagcggtag
     7921 cgccacgttc tcataggtgg tcatgtggtc gagcaggcgg aaatcctgga acacgacgcc
     7981 gatgcggcgg cgcagcaagg gcagttcggt gcgcgagatg cgggcgcggt ccttgccgaa
     8041 catggtgatc agcccgcgcg tcggcttcag cgacatgaac agcaggcgca gaagcgtcgt
     8101 cttgccggcg cccgaggggc cgctcaggaa ctggaacgag cgctccggta tgtgcaggga
     8161 aatgtcgcgg aggatttccg gacccatgcc atagcggaga ccgacatttt cgaagcggat
     8221 cacgttcgac gacctggttt atttcgggtg gcgaaccacc ctgtgttttc caaccgacca
     8281 tgggtgcgca tggttaaccg agcgttaaaa ttcatgggta ctaagcgtct cacgagggct
     8341 gcggtggcaa aatcttaacc attccgattt acgaaaatga aagatatgga aaacaggggc
     8401 gaataagagc cagcatggct gatgcgcgca cggcacgccc ggtttccggc gaaatcatga
     8461 cagatgccgc atcgggaagg gcattcggcc gcgcagcgcc ggctattgcg cccgatatca
     8521 tcgatgcgga ctacgaagtg ctgtcgcacg gcgaggagcg cgtgccggat gtccagccgg
     8581 cagcgcagcc gcgtatggtc gatgcgccgg ccgtggaagg catggacatg ctgcgcaagc
     8641 ccgatggggc gcccgcgccg gcgcgggttt cgcgcggcgg gccgatcttc tgggttgccg
     8701 gccttggcgc cgcgcttgcc gccttctggg tttcgggcgg cgacgcgctg gtgcgccatg
     8761 cgccgctttt tgccgtgcgt gacgcggcct attccgcgtt cagcatttcc ggcgtgaagt
     8821 cgcatgtcga tctttcccgg caaaatccgt tgctgttcat cgacggcgag gcttccaatg
     8881 acggaaacgg agcttccgcc ctgccgccgc tggaaatcag ggtgaccggc aatgacgggc
     8941 gcatcacgcg ctacacgctg gggacagccg ggcgtccgct ggctccgggt gaaagatttg
     9001 ccttttcgag ccggctcgac gtgcctaaga acggggtcag gaccgtttcg gtggcttttg
     9061 ccgaataggc gaaaattcaa gaggggcgca tcatgccagt cgttcgcggc aaggacatag
     9121 aggttctgtt ttcggcatcg gccattgccc gccgcaacct tgaactggcc aaggaaatcg
     9181 ccggcaacga ctatcacgac ctgctggtca tctcgatcct gaaaggctcc ttcattttcg
     9241 ccgccgacct catccgcgcc atgcacgatg tcgacctgtc gccggaaatg gaattcatct
     9301 tcatctccag ctatggtgcg ggcaccgcca gcggcgaggt gcgggtgctg cgcgatatcg
     9361 acaacgaggt cgccgggcgc gacattctgt tgatcgacga tattctcgaa tccggcaaga
     9421 cgctgacctt cacccgcgac ctgatgctgt cgcgcggcgc gaaaagctgt tcgatcgccg
     9481 tgctgctcga caagcgcatg cgtcgccaga ccgacctgaa cgccgattat gtcggcttcg
     9541 actgccccga tcacttcgtc gtcggctacg gcatggatgc cggccatgcc ttccgcgaac
     9601 tgccgttcgt gggcattgtg cgcggtgagg cgtaagcttc aggaaaattc aacttttcgc
     9661 cgccaagtat cggctcatgg caaaactcct gatcgttgag gacgacgctt ccgtccgcat
     9721 cctttgcgcc cgcgcgttcg aacgcgccgg ccatagtgtc gatgtggccg aggacggcgc
     9781 gcagggactg gcgcggatcg tcgaggcgcg gggcggctac agcctcgtcg tctcggatat
     9841 acggatgccg gaaatggacg gcatcgagat ggcaaaggcc gcggcggacg cgttccccag
     9901 cctgaagatt ctgttgatga ccggctatgc cgaccagcgc gaacgggccg aggacctgaa
     9961 cggcatcatc gtcgatgtcg tccagaaacc gtttacgctg gctgaaatcc gcgcgcgcgt
    10021 cgaccaggcg ctggcctgct tcgcctgacg cctcctgtgc ggcaggcgtg gctggaaagg
    10081 ctccggctta cttgcccttg gcgcgctcga cggcctcgac gatcatcttc ttggcgtagg
    10141 ctgcatcccg ccagccgcca agcttgaccc atttgccggg ttcgagatcc ttgtagtgct
    10201 cgaagaaatg cgccacctgg tcgagcgtga tctgcggcag gtcggtatag ttggccacct
    10261 tctcgtagcg cttggtgata tgcgaggaag gcacggcgat gaccttttcg tccatgccgg
    10321 cattgtcttc catcaacaaa acgccgatgg gccgtacatt gatgacgcag cccggcacca
    10381 gcgcgcgggt gttgcacacc agcacgtcga tcgggtcgcc gtcgccggac agcgtatgcg
    10441 ggacgaagcc gtaattgccg ggatagcgca tcggcgtata gaggaagcgg tcgacgacca
    10501 gcgtgccggc atccttgtcc atctcatatt tgatcggctc gccgccaacc gcaacttcga
    10561 tgatgacgtt gacgtcttcc ggcgggtttg acccggtggc gatggcttca atgcgcattg
    10621 aacggttccc tctttcgatc cgggaccgat agcggcaagt gcggcgacag gcaatgcggc
    10681 gaatggttcg cgcgcaagaa tggttcacgc atgaacggaa ggtgtcgcgg cagacgggcc
    10741 ggggatcatt cccagacgaa agcgactttc ttcagggcct tttgctcgaa gatctcgacg
    10801 ccttcggcaa catcgcgtcc gccggcggaa gcgtagaagg acaaggcgtt ctcattgtct
    10861 tccagcgccc atacgaccat gcccttcagg ccgttatcgg cgaggcgggc gcgggcggcg
    10921 gcaaacagcc ggctgccgag gccgatgccc tgatattcgg ggcgcaggta aagttcgtaa
    10981 atctcgccct gctggcgcaa ttcgcgggcg cgattcttgc ccagcgtcgt atagccggcg
    11041 atcttgccgc cgatctccac caccagcacg gtcgcggcgc ggcgaatggc gttggcccac
    11101 cagtcggacc cgcgccggtt gatcatggaa gtcagcgcgc gatgcgggat gatgccggca
    11161 taggcgccgt gccaggcctg ctggtgcaca tcggcaatcg cggcggcgtc gcgcggctct
    11221 gctcttctga tgtcgatggt cagcgtattc atgatttgtt aaccatagcc cggcgcccgc
    11281 caattgcaag atgccggaag gcgaggcctg gttctttcgc gcagccggcg cggcggaaac
    11341 gtcgatgcgg gcaggtttta cctgtgcggc gaccggcggc taagctgcgg aaaaaccggg
    11401 gaaaatccgc cgcaccgggc catccctaag ttggggtgcg gattagcgtt atctgcaagc
    11461 cattgcattt gaacggaaaa tatgatttta ttgaatggac attcaataaa tggaagagac
    11521 ataagatgaa cccgcacccg cgcgacgtgg ccattcccaa cgattgggac cgtcggggcc
    11581 tgccgggttg gagctatcat tccgacgccc ttctcgacct cgaaaaggag catgtcttcc
    11641 gcaatcactg gcagatagcc ggccatgttt cagacgtgcc gaacgcgggc gattacctga
    11701 ccatggatgt ggtcggcgaa cgcgccctga tcgtgcgcgg caagga
//
LOCUS       DASYCS010000113         3827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1473760,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000113 DASYCS010000000
VERSION     DASYCS010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1473760"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1267)
                     /locus_tag="VGM46_04010"
     CDS             complement(<1..1267)
                     /locus_tag="VGM46_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013530880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex family protein"
                     /protein_id="HEY4191789.1"
                     /translation="MQAKDRQARKSGARRRTRSRALFCALLGSTAFLAAGRSATAFEL
                     FGIKLWNSAADKEDAEIVDPLRYAVTIDAPDADKDLRKRIEAASALKGDEKRPVAGSL
                     GLLTKARSDREQLVAALYANAYYEGIVDISIAGKPLDGLPPDAEFDGAQPVPVVVKIQ
                     PGPKFTLGDIRLEGDAAGLASADYGLITGGDASSGAVLKAEARIVRALKEEGRPLAKV
                     TGREIVADHATSTLDVTLTLSAGPVAGYGETTVEGTEAVDRDFTAYMTGLKQGRKYSP
                     EEIDEARDRLLGMEVFSSVTLKEADKLDGGGNIPIGVQVSERKFRYFGLGGTVSNTEG
                     LGVEGYWGHRNLFGHAEKLRIDGSVSGIGSNDLNQLNYNAGIMFEKPGVIGPASKFFA
                     DLKTVYEHPDAYDRFSTRGGVGVSYELTPR"
     gene            1558..>3827
                     /locus_tag="VGM46_04015"
     CDS             1558..>3827
                     /locus_tag="VGM46_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HEY4191790.1"
                     /translation="MAMRAVRCLWVFAILLFALGGLAQADEAKRIVTTENSDYFGFDL
                     SSTQNISLDQCKTTCLGDAACRAFTYNNKARWCFLKSDFNSLKPFNGATAGKVVSLTT
                     DPDIGAPPALSFFPGWMAEQAQQYRNTLTGPGYQKPTDGLGALADAAAQAALAGDHRS
                     AMQKYEAAASVMPDDGRVWLELARQTLVVQPADSTETSNFQANATSAAFNAYKLLRTT
                     ATRADALSVLAQGLDKRDLYRPALQAYEASLSLVNSPTILAAYNDLKARKGFRIIDHS
                     VDADTSAPRVCAQFSEELVKSGVDYAQFVTVDGGAPKGIEAKDKQICVEGLEHGHHYD
                     VAFRAGLPAAIGEVIAAPTLLSIYVQDRAPSARFTGDSFVLPAGARRGIPLVSVNMKA
                     AQMKLFRIGDRALAQLLSGYQFLHQLDGYDVDSIADQLGAPVWEGQLEIAGDLNKEVT
                     TSFPVDEALPQRKPGVYVLTAQPVDDRSENYDSRATQWFVVSDIGLTTYTGEDGLNVF
                     ARSLATAKPIAGAELTLLAKNNEILGTARTDAEGRATFNPGLVRGEGGQVPAVLMARQ
                     GEQDFVFLDMSRAGFDLSDRGVEGRAAPGALDVYAWTERGIYRVGEDVHVAALARDAS
                     GTAVDKLPLTFIFSRPDGVEDRRIVSDGVSAGGHAVDLPLPGNAMRGTWSVSIYTDPK
                     QPAVASQMFLVDDFVPDRIEFDLTSDKQEIAQGETAKIGVDGRFLYGAPAVGLALEGE
                     LTLSTTRDWDRYKGFS"
BASE COUNT          688 a         1314 c         1131 g          694 t
ORIGIN      
        1 gtcggggcgt caattcatag gaaacgccga cgccgccgcg cgtcgagaag cggtcatagg
       61 cgtcgggatg ctcatacacc gtcttcaggt cggcgaagaa tttcgacgcc ggcccgatga
      121 cgcccggctt ttcgaacatg atgccggcat tgtaattgag ctggttcagg tcgttggaac
      181 cgatgccact gaccgatccg tcgatgcgca gcttctcggc atggccaaac aggttgcgat
      241 gcccccaata accctcgacg cccagccctt ccgtattcga aaccgtaccg ccaaggccga
      301 aatagcggaa cttgcgctcg ctgacctgca cgccgatggg aatgtttccg ccgccgtcga
      361 gcttgtccgc ctccttgagc gtgacgctgc tgaacacctc catcccaagc agcctgtcgc
      421 gcgcctcgtc gatttcctcg ggggaatatt tgcggccctg cttgaggccg gtcatgtagg
      481 ccgtaaagtc gcggtcgacg gcctcggtgc cctcgaccgt ggtttcgcca tagcccgcaa
      541 caggcccggc tgaaagcgtc agcgttacgt ccagcgtcga ggtggcgtgg tcggcgacga
      601 tctcgcggcc ggtcactttc gccagcggcc tgccctcttc cttcaaggcg cgcacgatcc
      661 tcgcctcggc cttcagaacg gcgccggaac tggcgtcgcc gccggttatc agcccgtaat
      721 cggcgctggc aagccctgcc gcatcccctt ccagcctgat atcgcccaag gtgaatttcg
      781 gccccggctg gatcttaacc accaccggaa cgggctgtgc gccatcgaat tccgcatccg
      841 gcggcaatcc gtccagcggc ttgccggcaa tgctgatatc gacgatgccc tcataatagg
      901 cattggcata aagggcggcg accagctgct cgcggtcgct tcgcgccttt gtaagcaggc
      961 cgagcgagcc ggccacgggc cgcttctcgt cgcccttcag cgccgaggcc gcctcgatcc
     1021 tcttgcgcaa atccttgtcg gcgtccggcg catcgatggt cacggcatag cgcagcggat
     1081 cgacgatctc ggcatcttcc ttgtcggcag ccgagttcca cagcttgatg ccgaacaatt
     1141 cgaaggcggt ggcggaccgg ccggcggcca gaaaggcggt cgatcccagc agcgcgcaaa
     1201 acagggcgcg cgaacgcgtg cgccttcgcg ccccgctttt tctcgcctgc ctgtccttcg
     1261 cctgcattcg gccgacccta gcgccaaaac ctaaaattga aatgaaagtc tgaactgcgc
     1321 gtaaaggagc aacgatccaa atgtctgaaa gtgcatgggc ttggaattca caccctgttg
     1381 cagccatgca agaaagcgtg acctgtcggg gcaggaaagc gtgagctatc agggatgtca
     1441 cggacttgtg cccggctaaa gaaaagcagg catgcgcatc gatttgttcc tcccgaaccc
     1501 gtgttgacaa ccgcaaccga agcctgaaca tgcatcggcg gcgactggag aggtgtcatg
     1561 gcaatgcgtg cggttcgttg tttgtgggtt ttcgccattc tccttttcgc ccttggcggc
     1621 ctggcgcagg ctgacgaagc caaacgcatc gtcaccacgg aaaattccga ctatttcggc
     1681 ttcgatctca gctcgacgca aaatatttcg ctcgaccagt gcaagaccac ctgccttggc
     1741 gatgccgcct gccgcgcctt cacctataac aacaaggcta ggtggtgctt tctcaaatcc
     1801 gacttcaatt cgctcaaacc cttcaacggc gccaccgccg gcaaggtcgt ctcgctgacc
     1861 accgatcccg atatcggcgc gccgccggcg ctttcctttt tccccggctg gatggccgaa
     1921 caggcgcagc aataccgcaa cacgctgacc ggccccggct atcaaaaacc gacggacggg
     1981 ctaggcgcac tggctgacgc cgccgcccag gcggctctcg ccggcgatca tcgcagcgcc
     2041 atgcagaaat atgaggcagc ggcttcggta atgccggacg atggccgcgt ctggctggaa
     2101 ctggcgcgcc aaaccttggt ggtacagccc gccgacagca ccgagacctc gaattttcag
     2161 gccaacgcca cttccgccgc gttcaacgcc tacaagctgc tgcgcacgac cgcgacccgc
     2221 gcggatgcgc tttcggtgct ggcgcagggg ctggacaagc gcgatctcta tcgcccggcc
     2281 cttcaggctt atgaggccag cctctcgctg gtcaactcgc ccaccatcct tgccgcctat
     2341 aacgacctca aggcgcgaaa aggcttccgc atcatcgatc attcggtcga tgccgacacc
     2401 agcgcgccgc gcgtctgcgc gcaattctcg gaggaactgg tcaaaagcgg cgtcgattac
     2461 gcccagttcg tcaccgtcga tggcggcgcg cccaagggca tcgaggccaa ggacaagcag
     2521 atttgcgtcg aaggcctgga acacggccac cactacgatg tcgccttccg cgccggcctg
     2581 cccgccgcga tcggcgaagt catcgcggcg ccgaccttat tgtcgatcta tgtgcaggac
     2641 cgcgcgccct ccgcccgctt caccggcgat agcttcgtgc tgccggccgg tgcgcggcgc
     2701 ggcatcccgc tggtcagcgt caacatgaag gcggcgcaga tgaagctgtt tcgcattggc
     2761 gaccgcgccc ttgcccagct tctgtccggc tatcagttcc tgcaccagct cgacggctat
     2821 gatgtggatt cgattgccga ccagcttggc gcgccggtct gggaaggcca gctcgaaatt
     2881 gccggcgacc tcaacaagga ggtcaccacg tccttccccg tggacgaggc cctgccccag
     2941 cgcaagcccg gcgtctatgt gctgaccgcg cagccggtgg acgaccgcag cgaaaactac
     3001 gattcccgcg ccacccagtg gttcgtcgtc tccgacatcg ggctcaccac ctataccggc
     3061 gaggacggat tgaacgtctt tgcccgctcg ctggccaccg ccaagccgat tgccggcgcg
     3121 gaactgaccc tgcttgccaa gaacaacgag atactcggca ccgccaggac cgacgccgag
     3181 ggccgcgcca ccttcaaccc cggccttgtg cggggcgaag gcgggcaggt gcccgccgta
     3241 ctgatggcca ggcagggcga acaggacttc gtgttcctcg acatgagccg cgccggcttc
     3301 gacctctccg accgcggcgt cgaaggccgc gccgcgcccg gcgcgctcga cgtctatgcc
     3361 tggaccgagc gcggcatcta ccgcgtcggc gaggatgtgc atgtcgcggc tcttgcccgc
     3421 gacgcctccg gcaccgcagt cgacaagctg ccgctgacct tcattttctc gcgccccgat
     3481 ggcgtggaag accgccgcat cgtcagcgat ggcgtttccg ccggcggcca cgcggtggat
     3541 ttgccgctgc cgggcaacgc catgcgcggc acctggtcgg tgtcgatcta caccgacccc
     3601 aagcagcccg ccgtcgccag ccagatgttc ctggtcgacg atttcgtgcc ggaccgcatc
     3661 gaattcgacc tgacctccga caagcaggaa atcgcgcagg gcgaaaccgc caagatcggc
     3721 gtcgatggcc gcttcctcta tggcgcgccg gccgtcggcc ttgcgctgga aggcgagctt
     3781 accctttcca ccacgcgcga ctgggaccgc tacaagggct tttcctt
//
LOCUS       DASYCS010000114         2804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2054699,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000114 DASYCS010000000
VERSION     DASYCS010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2054699"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1445)
                     /locus_tag="VGM46_04020"
     CDS             complement(<1..1445)
                     /locus_tag="VGM46_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY4191791.1"
                     /translation="MDIVKLAIRNSRLTISVLLFLVLAGAVAYQSVPKEAEPDVPIPM
                     MYVGLIYQGISPEDSERLLLRPMETKLKNLKGLKEMRSAAFQGGGYVLVEFEPSIDLA
                     TALQDTRSKVQDAKADLPQAAEEPTVNEVNISEFPVLVVTLSGHVPERALTAAARELR
                     DRIEEVPGVLEGALQGARDDLVEVVIDPVKLSSYGLQLDQLIQGVAASNNLVAAGNLE
                     GSEGKYAVKVPSLIETPEDVANLPVVTGPNAVVQAKDIATVRSTFKDAETITRLDGKP
                     AIAIEVKKRIGSNLIDTIAQVKTVADTFVKTMPKGMEVTYTQDKSVFVNQLLGDLQNH
                     VMIAVILVFIVILYALSGRASLLIGLAIPSSFLIGILLLALMGYTINMIVLFSLILAV
                     GMLVDDAIIVTEFAERRMGEGMPKEEAFALAAKRMAGPVIAATMTRIAAFSPLLFWPG
                     IIGDFMKYLPITLIVTLAASMLYALVFAPTLG"
     gene            complement(1447..2580)
                     /locus_tag="VGM46_04025"
     CDS             complement(1447..2580)
                     /locus_tag="VGM46_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY4191792.1"
                     /translation="MPKFRFHKLAALIVLIGFAAWVATGEFSSVGSASDTETPKAAKA
                     EQPAAPLRTVAVVTPPRAMHARAIRISGLTEADKRAVLATRVGGVIAKLPVKQGQHVK
                     AGDLILMLDAEEKLSSADNARQLLVQRQAELAAAERLAKSGNLPKLQLDTARSNLTSA
                     QSALRTAQAELDRNEVKAPFDGIVDRVPVELGSAVMQGGEVATVLALDPIVARGEISE
                     RDLRFIKIGDEADVRLVNGQMVKGTLRYISRDASAATRTFRVEIAVENADGAIPAGMT
                     AEITLRAAPTDAIVLPRSVATLSGSGDLGIRAVDKDNKVVFFPIDLIDDTSKGLVLAG
                     IPQDARVIVAGQELVAEGDVVEPVMADQETIRKLAGEAAAGTQ"
BASE COUNT          489 a          917 c          882 g          516 t
ORIGIN      
        1 cccagcgtcg gcgcaaacac cagcgcatag agcatcgacg ccgccagcgt cacgatcagc
       61 gtgatcggca gatatttcat gaaatcgccg atgatgcccg gccagaacaa aagcggcgag
      121 aaggcggcga tgcgcgtcat cgtcgcggcg atcaccgggc cggccatgcg cttggccgcc
      181 agcgcgaacg cctcctcctt cggcatgccc tcgcccatgc ggcgctcggc gaattcggtg
      241 acgatgatgg cgtcgtccac cagcatgccg accgccagga tcagcgagaa caagacgatc
      301 atgttgatgg tgtagcccat cagcgcgagc agcaggatgc cgatcaggaa cgaggatgga
      361 atggccagcc cgatcagcag cgaagcgcgg cccgacaggg catagagaat gacgatgaac
      421 accaggatca ccgcgatcat gacgtggttc tgcaaatcgc cgagcaactg gttgacgaat
      481 acggacttgt cttgcgtgta ggtcacctcc atgcccttcg gcatggtctt gacgaacgtg
      541 tcggccaccg tcttcacctg cgcgatcgtg tcgatcaggt tggagccgat gcgcttcttg
      601 acctcgatgg cgatggccgg cttgccgtcc agccgcgtga tcgtctccgc gtccttgaag
      661 gtcgagcgga ccgtggcgat gtccttggcc tgcaccaccg cgttcggccc cgtcaccacc
      721 ggcaggttgg cgacatcttc cggcgtctcg atcagcgacg gcaccttgac ggcatatttg
      781 ccttccgatc cttccagatt gccggcggcc accagattgt tggaagccgc cacgccctgg
      841 atcagctggt cgagctgcaa gccgtaggac gacagcttga ccggatcaat gacgacttcg
      901 acaagatcgt cgcgcgcgcc ttgcagcgcg ccttccagca cgcccggcac ttcctcgatg
      961 cggtcgcgca attcgcgcgc cgccgccgtc agcgcgcgct ccggcacatg gccggaaagc
     1021 gtcaccacca gcaccggaaa ttcggagatg ttgacctcgt tgacggtcgg ctcttcggcg
     1081 gcctgcggca ggtcggcctt ggcgtcctgc accttggagc gcgtgtcctg caacgcggtc
     1141 gccaggtcga tggacggctc gaactcgacc agcacgtagc cgccgccctg gaaggcggcc
     1201 gaacgcattt ccttcaaccc cttgaggttc ttcagcttgg tttccatcgg ccgcagcagc
     1261 aggcgctcgg aatcctccgg cgagatgccc tgatagatca ggccgacata catcatcggt
     1321 atcggcacgt ccggctcggc ttccttcggc accgactggt aggcgaccgc ccccgccaga
     1381 acgaggaata gcaggacgga aatggtgagc cgcgaattgc ggatcgctag tttgacgata
     1441 tccatattat tgcgttccgg ccgcggcttc gccggccagc ttcctgatgg tttcctggtc
     1501 ggccatcacc ggctcgacca catcgccttc agcgaccagc tcctgtccgg cgacgatcac
     1561 cctggcgtcc tgcgggatac cggcaagcac cagccccttg gacgtgtcgt cgataaggtc
     1621 gatcgggaag aacaccacct tgttgtcctt gtcgacggcg cggatgccga gatcgcccga
     1681 gccgctcagc gtcgccaccg agcgcggcag cacgatggcg tcggtgggcg ccgcccgcag
     1741 cgtaatttca gcggtcatgc cggcgggaat ggcgccatcg gcattctcga cggctatttc
     1801 aacgcgaaag gtgcgggtcg ccgcagaggc gtcgcggctg atgtagcgca gcgtgccctt
     1861 caccatctgg ccgttgacca gcctgacgtc tgcttcgtcg ccaatcttga tgaagcgcag
     1921 gtcgcgctcg ctgatttcgc cgcgcgcgac gatcggatcg agcgcaagca ccgtggcgac
     1981 ttccccgccc tgcatcaccg cgctgcccag ttccaccggc acgcggtcga cgatgccgtc
     2041 gaatggcgcc ttgacctcgt tgcggtcaag ttcggcctgc gcggtgcgca acgccgactg
     2101 cgcggacgtc aggttggagc gcgccgtatc gagctggagc ttgggcagat tgccggattt
     2161 ggccaggcgc tcggccgccg ccagttcggc ctgccgctgc accagaagct gcctggcatt
     2221 gtcggcggac gaaagctttt cctcggcgtc gagcatcagg ataaggtcgc ccgccttcac
     2281 atgctgtccc tgcttgaccg gcagcttggc gatgacgccg cccacccgcg ttgccagcac
     2341 ggcgcgcttg tcggcttccg tcagccccga aatgcggatg gcgcgcgcat gcatggcgcg
     2401 cggcggcgtc accacggcaa ccgtgcgcag cggggccgcc ggctgttcgg ccttggccgc
     2461 cttcggcgtt tcggtgtcgg aagcgcttcc caccgacgag aattcgcctg tcgccaccca
     2521 ggcggcgaag ccgatgagca caataagggc ggcaagcttg tgaaacctga atttcggcat
     2581 tgtcgtcttt ccggtgtgcg gggcgtggcc ggccgcatct tcattcggcg cgagcctgat
     2641 atgggtgcgc gcgcgttccg ttcaaatcgc cgtgatcggc ggagcgcacc ttctacagga
     2701 aagttacggc gcaaaatagt cggaaagatt gaggcgatat tcgccggcgg acgaatgaac
     2761 aggctctaaa acggctgaaa tttctgcatt ggagccgttt tccg
//
LOCUS       DASYCS010000115        17936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3070299,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000115 DASYCS010000000
VERSION     DASYCS010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 17936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17936
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3070299"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /gene="iolE"
                     /locus_tag="VGM46_04030"
     CDS             <1..880
                     /gene="iolE"
                     /locus_tag="VGM46_04030"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018429217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050114 - myo-inosose-2 dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HEY4191793.1"
                     /translation="WNDDLAELSDDVSLEECLAQSRSAGFTGMEMGRRFPGDPKLMLP
                     ILEQADVTLCGGWFSGTLVNEDMAVNRDRIQPMIELFKAVGAPCIVYGEVGRSIQGDR
                     SKPLSTKPRLSDAEMRVYAKKLTEFGEWCAGQGMPLAYHHHMGAVVETETELDAFMNH
                     SGPGIPLLLDAGHLAFAGGDVLRAIDNHHARISHVHVKDVRMAVIDKLDRARQSFLDA
                     VALGAFTVPGDGSLDFEAIVKQFADHGYEGWFVVEAEQDPKKNPPLKMAQVGHKELMR
                     VMTAAGYTVETQGFPS"
     gene            910..1173
                     /locus_tag="VGM46_04035"
     CDS             910..1173
                     /locus_tag="VGM46_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3329 domain-containing protein"
                     /protein_id="HEY4191794.1"
                     /translation="MAQIKDTQHPFFRPLWRRIAIVVVCLVWAGLEYIGGSASWGTIA
                     LAAAVYGAWQYLYLYKPGPESVPSPDAVAPVDGVQPADSEPKG"
     gene            1176..1991
                     /gene="iolB"
                     /locus_tag="VGM46_04040"
     CDS             1176..1991
                     /gene="iolB"
                     /locus_tag="VGM46_04040"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HEY4191795.1"
                     /translation="MPQLIIRPKKGGYGRLVHVTPQNAGWTYVGFDLHRLRPGETVSA
                     QTGSREVCLVFVTGKGKASAGGKQLGSLGERMSPFEGKPWSLYLPEGSEWSVTAETEL
                     ELAVCSAPGLGGGLPVRVIAPGDLGRETRGKGTNTRYVTNILPEDEPADSLLVVEVIT
                     PGGHTSSYPPHKHDQDNLPAESALEETYYHRLDPPQGFAFQRVYTDADEHGRRALDEA
                     MAVEDGDVVLVPKGYHPCAACHGYDLYYLNVMAGPKRTWKFHNAVEHEWLMKA"
     gene            complement(1993..3099)
                     /locus_tag="VGM46_04045"
     CDS             complement(1993..3099)
                     /locus_tag="VGM46_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1513 domain-containing protein"
                     /protein_id="HEY4191796.1"
                     /translation="MRTPLIDRRDFLKAAGAGFAAALAPPAWAATLAADAVFATAFVR
                     RNGSYGAGILSEAGKLLYSIELPDRGHDVTFDAISRRSVVFPRKPGTIAVVFDHTGQA
                     KPLTIAAIAGRHFYGHGVFSHDGALLYATENDFDNAAGMIGIYDARNDFARIGEFATH
                     GVGPHELMLLNDGRTLAIANGGIETHPDFGAAKLNLATMQPSYVLIDRVSGDLLEKHQ
                     LPPDLNQLSIRHMDADRQGAIWFSCQYEGATTDRPSLVGRAVRGKELELLEMPEKVLN
                     GLHNYIGAMAANREAGTMAVSSPAGNSYVVFDTGSGKVVSATDLTEVCGIAPDRKGFI
                     ATSGAGMIVAAGSTGVSEPDYVWDNHMKRIIETG"
     gene            complement(3120..4211)
                     /locus_tag="VGM46_04050"
     CDS             complement(3120..4211)
                     /locus_tag="VGM46_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019863058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HEY4191797.1"
                     /translation="MMKRFAFPLTFALALPLALSAIAPASAAIKASIVINAAIDGFVR
                     PAYAGLHEHAATLTKAMQTLCKAPSQANLDAARSEFSGAVDAWSKAEIITFGPIREKN
                     RLERMLFWPDRKSIGLKQVQAALAGKDATATDPAQLAGKSVAMQGFGALEFVLYGTDA
                     DGLTGKDDAYRCAYGAAVAGNIETMAGDIAAEWDKKDGFAALWANPGPDNPLYRDGNE
                     AVTELMGIFINELEMVRDVRLKGFFGATPDADKPKQAIYWRSANTTASLAANLKGIDQ
                     LLTVSHVGDALSPDARWMADSVHILLANGAADARGVTGPLDEALADPARRARLDHFAL
                     VTSTLSGLIGTRMTAEFGLTAGFSSLDGD"
     gene            complement(4211..5812)
                     /locus_tag="VGM46_04055"
     CDS             complement(4211..5812)
                     /locus_tag="VGM46_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010913301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HEY4191798.1"
                     /translation="MRRPLELLRRRRRAEDCPPKTNRKSALALSGLVAGVLAVFSLSA
                     LAGSDADGLAVTRSDLTPKDLARVEAITKPTHDFSKAEQFELMQGGAGTSKKRVNADA
                     FSQSSANITFEEEGTFKLGNALFRKNWVSSPSSTQASDGLGPLFNARACQSCHLKDGR
                     GHPPEASLDATSMFLRLAREADTAEEKAKVASHQALNFPDPVYGTQLQDLAVPGISGE
                     GRMHIAYREIPVALGGGATASLRQPTYSATDLAYGPLGPHTTLSPRVANPMIGLGLVE
                     QIHPADILALADPDDKNGDGISGKAAIVRDPLTGQPTLGRFGWKAQQPTIRAQSADAL
                     AGDIGISSPEAPRPWGDCTEAETACRAMPTGVQPRLGDTEAPVPVMDLITFYSQNLAV
                     PARRDIGKPDILAGKQAFYDMGCVSCHTPKFVTRRDAPNKAQAFQLIWPYSDFLLHDM
                     GDGLADGQQVGIATGNEWRTPPLWGIGLTKRVNGHTFFLHDGRARNLTEAILWHGGEG
                     QKARDRFAAADAATRDALVKFLESL"
     gene            complement(5825..6310)
                     /gene="bfr"
                     /locus_tag="VGM46_04060"
     CDS             complement(5825..6310)
                     /gene="bfr"
                     /locus_tag="VGM46_04060"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012759495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEY4191799.1"
                     /translation="MKGETQIIERLNEALFLELGAVNQYWVHFRLLEDWGYTKLAKKE
                     RAESIEEMHHADRLIARIIFLEGHPNLQSVAPLRIGQNVKEVLESDLSGEYEARTAYK
                     RSREICNELGDYVTMKLFEELLADEEGHIDFLETQLDLLASIGDEKYGQLNADAANEA
                     E"
     gene            complement(6276..6587)
                     /locus_tag="VGM46_04065"
     CDS             complement(6276..6587)
                     /locus_tag="VGM46_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002966070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY4191800.1"
                     /translation="MLICQCNVITQKEVEQAIVELLDQDPWQLIVPAKVYHSIHKRGR
                     CCGCFPNVVETIIRVTENYHARSQASGVDIVSHLDRVRELRVQYGSTAHERRNPNHRA
                     A"
     gene            complement(6661..7953)
                     /locus_tag="VGM46_04070"
     CDS             complement(6661..7953)
                     /locus_tag="VGM46_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006201121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HEY4191801.1"
                     /translation="MLNNRYAGRFAALAATAALTAAVFVLPAKADTDAKAVIKTYSDI
                     ALAKYEDSLTTAQALDKAVDALIAKPSADTLTAAREAWKAARIPYQQTEVYRFGNPIV
                     DDWEGRVNAWPLDEGLIDYVAKSYGTESDQNSLYTANVIANKTIKIDGKEIDASKLTP
                     EFLSGTLQEAGDIEANVATGYHAIEFLLWGQDLHGTGPGAGERPYTDYDAANCTGGNC
                     DRRADYLKSASDLLVSDLQEMVGNWKEGGAARKNLEDGEPNAGISTILTGMGSLSYGE
                     LAGERMKLGLLLHDPEEEHDCFSDNTYISHLEDAVGIRAAYHASYTRIDGSVVSGPSV
                     SDMVKAADPALDKELSDKLDLTVSKMEAIKARALGGEAYDQQIAEGNTEGNATVQAAI
                     DGLVDQTKSIERVVSALKLDAIAFEGSDSLDAPDKVFQ"
     gene            8427..9665
                     /locus_tag="VGM46_04075"
     CDS             8427..9665
                     /locus_tag="VGM46_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY4191802.1"
                     /translation="MNDGVVIVGAGHAGVQAAASLREEGYDGPVTLVGDENELPYHKP
                     PLSKTFIKDAQAKPQPLRGEAFYSGNAIDFRPGVSVERIDLGQRRLQLAGGGALPFDR
                     LILATGSRPRPLPLPGSDLAGVLSLRSIGDARLIRDQSGRCTEVVILGGGFIGLEIAA
                     TLTAGGRKVTVVEAADRLLGRAVAPAIGAHARQRLEDIGVRILTGTTIARLEGENGHV
                     VAAVTSTGERLPAEMVIVGIGVVPNAELAQAAGIATGNGIKVDAAMQTSVPQVLALGD
                     AASYRHWFTGADVRLESVQNATDQARLAARTITGKGGAYAAVPWFWSDIGDMKLQMVG
                     LTSSSDRNIVVGDPAENKFSIYHYADDRLIGIESVNRPGDHMLGRKMLGAGFSPLPET
                     VSTGPDGLKAALAAFQQETQ"
     gene            complement(9761..10861)
                     /locus_tag="VGM46_04080"
     CDS             complement(9761..10861)
                     /locus_tag="VGM46_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine:D-lactate ligase-like protein"
                     /protein_id="HEY4191803.1"
                     /translation="MSSSQSKLAQSVPAQLILVYETEKACFDRLVGDGHPPARAAEIS
                     SYLAQSADLAPDFEALAAACTRHDIVFTPVDLDDAAKALARRDPLKTLVWTLTDGIAY
                     FRGGASPALARLNGLPTLGSDDALFALCQDKFRSGAVLAALGLPAPAAGLARNGQWLV
                     TPPASAQGWFVKPNRLGAKIGIWADSRCHDLADALELSRRIFSAYRDEVVVQPYVAGR
                     NVRASFLGLERNAGIEALGVAFVDSGADFQTMEDSLALYGKTGEAARAEGRYAEPELV
                     PVKASQPGADMKIRRIAQTLMQSLGLRDVFSIDLRVEADDTVHLIEFEVCPGLPCFDF
                     RAYCRAQWNMSLADAMAATAAARLASTGDRQA"
     gene            complement(10888..11655)
                     /locus_tag="VGM46_04085"
     CDS             complement(10888..11655)
                     /locus_tag="VGM46_04085"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006205380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY4191804.1"
                     /translation="MMPSARFADLEGASVLITGGGSGIGARLTEGFARQGSKVAFIDI
                     ADKQSLALCDEIEAATGNRPLYLHADLRDIAALRASVAKAGTAHGPITVLVNNAARDD
                     RHLAEELTPEDWDANLETNLRPHFFTMQAVAPGMRKAGHGSIVNFTSTAFMLNIGDMP
                     AYTAAKAGIVGLTKGLAGRYGKDGIRVNAIAPGWVITERQNELWVTRESLDRHISRQA
                     IPRMMLPDDMVGPCLFLASDASAMLTAQTLIVDGGLL"
     gene            complement(11765..13114)
                     /locus_tag="VGM46_04090"
     CDS             complement(11765..13114)
                     /locus_tag="VGM46_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY4191805.1"
                     /translation="MQTSFGTGQAGREGSDLAFTDPLTGLGNHRRFFDKVERLISDRS
                     EDPAPFAVGIVDLDGFKPINDLFGHKAGDDILVQVAMRLNASMDGHSTVCRIGADEFA
                     FLYPMVFSEEAATKRARMLIEILSAPYDVGERTARLSASVGCSLFYSGDEATEVLIDK
                     AETALYHAKRSGRGQVVVYTREMEEAAKRVTRIEQALRRAVSAGEVEPHFQPIVDLNS
                     RRTIGFETLARWTDRDLGPVSPAVFIPIAEERGIIGPLSQLVLRKATEAARNWPKDLF
                     LAFNLSPSQLVDQNTGLHILSILDRTGFDPRRLEIEITETGLMNDPSSAGKTVEDLRR
                     VGIRVSLDDFGTGQSSLGRLREFHFDKLKIDRAFVSSILDDRPSEHIIRAILAMCEGL
                     GMDVVAEGIEEEAQADRLIQFGCAGGQGYLFGKPVNANATLGYLRDSFRGASRTSAL"
     gene            complement(13235..15112)
                     /locus_tag="VGM46_04095"
     CDS             complement(13235..15112)
                     /locus_tag="VGM46_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006205382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEY4191806.1"
                     /translation="MKPHRLGSVFAIDPKVVHVFQRAMSSSAVTTFRASRAAWSQDLG
                     LALIATLIALAVDAMSGFKTLHDAADNDSLLRLIEIRDLLAGQGWFDLHQYRMGVDGG
                     FVMHWSRLVDAPIAAIILAASAFTGSIAGGEMAARILWPALMLCLSVFFLARAARRYG
                     GDSAVLPAVIAGGGALHFLGIFSPNALDHHNIQLMLTLASLSFLLAAPQQKAAAAFSG
                     ICAALTLAIGMETAPYVAVIGLCVAGLFLFGGQGERSVARQYGLGFAGTALTVFVSTI
                     PFADWGQARCDAFSSVQFVLAALGGTGLAVAASFDAACRTPMRRAMALAVLGAGLAAI
                     TLHWFPQCLAAPYAGLDPRLHEYWLDHISEAQSMFQLIQARDSSVLARYVTPIIAIAI
                     MAARLRQGAWRRQDSLVGALLVASFVVSIWQVRGSTFSIALAVIALSGWIASWRERAL
                     ATPTPAVSLKMIAVWLVSMNAFWTVASAMIADDPSATGAPVAGKPAMADCKADKDFSI
                     LATQPDSTVLATSNLGAPVLAYSGHRALSGPYHRNIAGNLLVLDAYMGTIAEAKTVID
                     RNKVGLVALCPGSPENKLLVDGAPGGFLAQLLRGAIPAWLEPVAGAQDGPLQLYRVKQ
                     G"
     gene            complement(15136..15534)
                     /locus_tag="VGM46_04100"
     CDS             complement(15136..15534)
                     /locus_tag="VGM46_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013891989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEY4191807.1"
                     /translation="MGAILHGAGGQRLVRFVVVGVGAAALFFGLSWLLVSFGVPPFIG
                     SAIAYGSAFCVAYCAQRSWTFEGRHDHGQVLPRYLTLQLGCALFSGVLAHVAVTRFGM
                     APLAMSAFTTLAASAASYVLSTFWVFADRR"
     gene            complement(15542..16771)
                     /locus_tag="VGM46_04105"
     CDS             complement(15542..16771)
                     /locus_tag="VGM46_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY4191808.1"
                     /translation="MNAVTPTGSAATRQMELTLLMPCLNEAETLAVCINKARAFLDAS
                     GIIGEILIADNGSTDGSQAIATALGARVVPVAQKGYGAALLGGIASARGRFVIMGDAD
                     DSYDFSALGAFVARLREGADLVMGNRFQGGIEPGAMPPLHRYLGNPVLSFIGRLFFRI
                     KAGDFHCGLRGFNTEAIRKLDLQTTGMEFASEMVVRSALANLRIEEVPTTLKPDGRSR
                     PPHLRTWRDGWRHLKFLLVYNPRWLFFVPGAFLCAVGAVFAALLLFGPLRVIDNMSLD
                     LNTFTAACFMILTGVQLLTFGVISRCFAEKTQMLPSTRRSNWLSGTISTESLAIDAGI
                     CFFGGALFFGYAVLQWAHVGFGPLNNSEIPRVVIMGLTLIVISLQLFFSAFLLGVLEI
                     PLKRLAAAGTGRADSAL"
     gene            16988..17932
                     /gene="gshB"
                     /locus_tag="VGM46_04110"
     CDS             16988..17932
                     /gene="gshB"
                     /locus_tag="VGM46_04110"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEY4191809.1"
                     /translation="MSMKVAVQMDHVSTVSIMGDTSFALSLEAQRRGHRLFHYTPDRL
                     SMRDGKVFARIEEMQLRDEKANHYTLGEPVRTDLSEMDVILLRQDPPFDMNYITTTHM
                     LERIHPKTLVVNDPAWVRNSPEKIFVTEFPDLMPETLITKDPREVEAFRKEFGDIIIK
                     PLYGNGGAGVFQLNDGDRNLTSLLEMFSQMFREPFIVQRYLKDVRKGDKRIILIDGEP
                     VGAVNRVPSETESRSNMHVGGRAEKSELTDREREICARIAPSLKARGFILVGIDVIGD
                     YMTEINVTSPTGIREIKRFGGADIAGLFWDAVEDKRRS"
BASE COUNT         3331 a         5575 c         5862 g         3168 t
ORIGIN      
        1 gtggaacgac gatcttgccg aattgagcga cgatgtttcg ctggaagagt gcctggccca
       61 atcgcgttcg gcaggcttca ccggcatgga aatgggccgg cgctttccag gcgatccgaa
      121 gcttatgctg ccgatcctcg aacaggccga tgtgacgctt tgcggcggct ggttttccgg
      181 cactctcgtc aatgaggata tggcggtaaa cagggaccgc atccagccga tgatcgagct
      241 gttcaaggcc gttggcgcgc cctgcatcgt ctatggtgag gtcggccgct ccattcaggg
      301 cgaccgctcg aagccgctga gcaccaagcc gaggctttcc gacgccgaaa tgagggtcta
      361 tgcgaagaag ctgacggaat tcggcgaatg gtgcgccggg cagggcatgc cgctcgccta
      421 tcatcaccac atgggagcgg tcgtcgagac cgagacggaa ctggacgcct tcatgaacca
      481 ttcaggccca ggcatccccc tgctgctcga tgccggccac ctggcgtttg ccgggggcga
      541 cgtgctgcgc gccatcgaca accaccatgc ccgcatcagc cacgtccatg taaaggatgt
      601 gcgcatggcg gtgatcgaca agctcgaccg cgcgaggcag tccttcctcg acgccgtggc
      661 gcttggcgct ttcaccgttc cgggcgatgg ctcgctggat ttcgaggcta tcgtcaagca
      721 atttgccgac catggctatg aaggctggtt cgtcgtcgaa gcggaacagg acccgaagaa
      781 gaatccgcca ttgaagatgg cgcaggtcgg ccacaaggag ttgatgcggg tgatgaccgc
      841 cgccggctat actgtcgaga cgcagggctt tccgtcctga tagtagcccc gcaatatcgg
      901 aattgatcaa tggcgcagat aaaagacacc cagcatccat tcttccgccc gctctggcgg
      961 cggatcgcca tcgtcgtggt ctgccttgtc tgggcggggt tggaatatat tggcggctct
     1021 gccagctggg gcaccatcgc gctggcggcc gctgtctatg gcgcgtggca atatctgtat
     1081 ctctacaaac ccggtcccga aagcgtgccg tcacccgacg ccgtcgcgcc agtcgatggc
     1141 gtgcagcctg ccgatagcga gccgaagggt tagccatgcc gcaactcatc atccgcccaa
     1201 aaaagggcgg ctatggccgc ctcgttcatg tgacgccgca aaacgccggc tggacctatg
     1261 tcggcttcga tctccaccgc ctgcgccccg gcgaaacggt ctcggcgcaa accgggagca
     1321 gggaggtctg tctggtgttc gtgaccggca aggggaaggc cagcgccggc ggcaagcagc
     1381 ttggctcgct tggcgagcgc atgtcgccgt tcgaaggcaa gccgtggtcg ctctacctgc
     1441 cggaaggttc cgaatggtcg gtcaccgccg aaaccgagct tgagcttgcc gtctgttcgg
     1501 cgcccggcct tggcggcggc ttgccggttc gcgtcatcgc gccgggcgat cttggccggg
     1561 aaacccgcgg caagggcacc aatacgcgct atgtcaccaa catattgccg gaggacgaac
     1621 cggcggattc gctgctggtg gtcgaggtga taacgcccgg cggccacact tccagctacc
     1681 cgccgcacaa gcatgaccag gacaatctgc cggctgaatc cgcgctggag gaaacctact
     1741 atcaccggct cgacccgccg cagggctttg ccttccagcg cgtttatacc gatgccgatg
     1801 agcacggccg gcgcgcgctg gacgaggcga tggcggtgga ggatggcgac gtggtgctgg
     1861 tgccgaaggg ctaccacccc tgtgccgcct gccacggcta cgatctctat tatctcaacg
     1921 tcatggccgg gccgaagcgg acgtggaagt tccataacgc cgtcgagcac gagtggctga
     1981 tgaaggcctg aactatccgg tctcgataat gcgcttcatg tggttgtccc agacatagtc
     2041 cggttccgac acgccggtgc tgccggccgc cacgatcatg ccggcgccgc tggtggcgat
     2101 gaagcctttg cggtccggcg cgatgccgca cacttcggtg aggtcggttg cagaaacgac
     2161 cttgccgctg cccgtatcga acacgacata gctgttgccg gcgggtgacg acaccgccat
     2221 cgtgccggct tcgcggttcg ccgccatcgc gccgatgtaa ttgtgcaggc cgttcagcac
     2281 tttttccggc atttcgagca gttccagttc cttgccgcgc acggcgcgcc cgacaagcga
     2341 tggccggtcg gtcgtcgctc cctcatattg gcagctgaac cagatcgcgc cctgccggtc
     2401 ggcgtccatg tggcggatgg aaagctggtt caggtcgggc ggcaattggt gcttttccag
     2461 cagatcgccg gaaacgcggt cgatcagcac gtaggatggc tgcatcgtcg ccaggttgag
     2521 cttggccgcg ccgaaatccg gatgcgtttc gatgccgcca ttggcgatgg ccagcgtgcg
     2581 gccgtcgttg agcagcatca gttcgtgcgg cccgacgccg tgggtcgcaa actcgccgat
     2641 gcgggcaaaa tcattgcggg cgtcatagat gccgatcatg ccggcggcgt tgtcgaaatc
     2701 gttttcggtc gcataaagca gcgcgccatc atgcgaaaac acgccgtgcc catagaagtg
     2761 gcggccggcg atggcagcaa tggtcagcgg cttggcttga ccggtatggt cgaagacgac
     2821 ggcgatggtg ccgggcttgc gcggaaacac cactgaacgc cgcgatatgg cgtcgaaggt
     2881 cacgtcatgg ccgcggtcgg gaagctcgat cgaatagaga agcttgccgg cctccgaaag
     2941 aatgcccgca ccataactgc cgttgcgcct gacgaaggcg gtggcgaaca ccgcatcggc
     3001 ggcgagcgtc gccgcccagg ccggtggcgc aagcgctgcc gcaaagccgg cgccggcggc
     3061 cttgagaaaa tcgcggcggt cgatcagcgg cgtacgcatg gtatgttgcc tcgcttggct
     3121 caatcgccat ccagcgacga gaacccggcg gtcaggccga attcggcggt catgcgcgtg
     3181 ccgatcaggc cggaaagcgt gctggtcacc agcgcgaaat gatcgagcct ggcgcgccgc
     3241 gccggatcgg ccagcgcctc gtcgagtggc ccggtaacgc ccctcgcatc ggcggccccg
     3301 ttggcaagca ggatgtggac cgaatccgcc atccagcgcg cgtccggcga aagggcgtcg
     3361 ccgacatgcg agacggtgag caactggtcg atgcctttga ggttggcggc cagcgaggcg
     3421 gtggtgttgg ccgagcgcca gtagatcgcc tgcttcggct tgtcggcatc gggcgtggcg
     3481 ccgaagaagc ccttcagacg cacgtcgcgc accatctcaa gctcgttgat gaagatgccc
     3541 atcagttcgg tgacggcctc gttgccgtcg cggtaaagcg gattgtccgg tccgggattg
     3601 gcccagagcg ccgcaaaccc gtccttcttg tcccattcgg cggcaatatc gccagccatc
     3661 gtctcaatat tgccggcaac cgccgcgcca taggcgcaac gataggcgtc gtccttgccg
     3721 gtgaggccgt ccgcgtctgt cccgtaaagt acgaactcca gcgccccgaa cccctgcatg
     3781 gcgacgcttt tgccggcaag ctgcgccggg tcggttgcgg tggcatcctt gccggcaagc
     3841 gctgcctgca cctgcttcag cccgatcgac ttgcggtcgg gccaaaacag catgcgttcc
     3901 agccggttct tctcgcggat cggcccgaag gtgatgatct ccgccttcga ccatgcatcg
     3961 acagcaccgg aaaattcgct gcgcgccgca tccagattgg cctgcgatgg cgccttgcag
     4021 agcgtttgca tggccttggt tagcgtggct gcgtgttcgt ggaggccggc gtaagcgggc
     4081 cgcacgaaac cgtcgatggc ggcgttgatc acaatggatg ccttgatcgc cgccgaggcg
     4141 ggcgcaatgg ccgacagggc gagaggcaag gccagcgcga aggtcagggg gaaggcaaac
     4201 cgcttcatca tcacagggac tccaggaatt tgaccagcgc atcgcgcgtg gcggcgtcag
     4261 cggcggcgaa acggtcgcgg gccttctgcc cttcgccgcc atgccacagg atcgcctcgg
     4321 tcaggttgcg ggcgcgcccg tcatgcagga agaaagtgtg gccgttgacg cgcttggtca
     4381 gcccgatgcc ccacagcggc ggcgtgcgcc attcattgcc ggtagcaatg ccgacctgct
     4441 ggccgtccgc cagtccatcg cccatgtcgt gcaaaaggaa gtcggaatag ggccagatca
     4501 gctggaaggc ctgcgccttg ttgggggcgt cgcggcgggt cacgaatttc ggcgtgtggc
     4561 acgaaacgca gcccatatcg taaaacgcct gcttgccggc gagaatgtcc ggtttgccga
     4621 tgtcgcgccg cgccggcacg gcaaggttct gcgaatagaa ggtgatgagg tccatcaccg
     4681 gcaccggcgc ttcggtgtcg ccaagccgcg gttgcacgcc ggtcggcatg gcgcggcagg
     4741 cggtttcggc ctccgtgcaa tcgccccaag ggcgcggtgc ttccggtgac gaaataccga
     4801 tatcgccagc cagcgcatcg gccgattgcg cgcggatggt cggctgctgg gctttccagc
     4861 cgaagcggcc aagcgtcggc tgcccggtca gcggatcgcg cacgatggcc gccttgccgg
     4921 aaatgccgtc gccattcttg tcgtcggggt cggcaagcgc cagtatgtcg gcgggatgga
     4981 tttgctcgac cagccccagc ccgatcatcg ggttggcgac gcgcggcgac aaagtggtgt
     5041 gcgggccgag cggcccgtat gcgagatcgg tggccgaata ggtcggctgg cgcagcgagg
     5101 ctgtcgcgcc gccgcccagc gccaccggta tttcccgata ggcgatgtgc atgcgccctt
     5161 cacccgaaat gccgggcacc gcgagatcct gcaactgcgt gccatagacc gggtcgggaa
     5221 aattcagcgc ctgatggctg gcgaccttgg ccttttcctc cgccgtatcc gcctcgcgcg
     5281 ccagccgcag gaacatcgag gtggcgtcga ggctggcttc cggcggatgg ccgcgcccgt
     5341 ccttcaggtg gcaggattgg caggcgcgcg cgttgaacag tgggccaagc ccgtccgatg
     5401 cctgcgtcga agagggggaa gacacccagt tcttgcggaa aagcgcgttg ccgagcttga
     5461 aggtgccttc ttcctcgaag gtgatgttgg ccgaggattg cgagaaggca tcggcattga
     5521 cgcgcttctt cgacgtgccg gcgccaccct gcatcagctc gaattgctcg gccttggaaa
     5581 agtcatgggt gggcttggta atggcctcga cccgcgccaa atccttgggc gtcaggtcgg
     5641 agcgtgtgac ggcgaggccg tcggcatcgg agccggccag cgcagagagg gagaaaaccg
     5701 ccaatacccc ggcaaccagg ccggaaagcg ccagggcgct tttccggttg gttttgggcg
     5761 ggcaatcttc ggcccgccgc ctgcggcgca acaattctaa ggggcgccgc attcccgcat
     5821 gtccttactc cgcctcgttg gccgcgtcgg cgttgagctg gccgtacttt tcgtcgccga
     5881 tggaagccag gaggtctagc tgcgtctcaa ggaagtcgat gtgaccttcc tcatccgcca
     5941 acagttcctc gaacagcttc atggtgacgt aatcgcccag ctcgttgcag atttcgcgcg
     6001 aacgcttgta ggcggtgcgc gcttcatatt cgccggaaag atcggattcc agcacttcct
     6061 tcacattctg cccgatgcgc agcggcgcga ccgattgcag gttcggatgg ccttcgagga
     6121 agatgatgcg ggcgatcagc ctgtcggcgt ggtgcatctc ctcgatcgat tccgcccgtt
     6181 ccttctttgc cagcttggtg tatccccagt cctccagcag acggaaatgc acccagtact
     6241 gattgacggc tccaagctcc agaaacaggg cttcgttaag ccgctcgatg atttgggttt
     6301 cgcctttcat gggccgtact cccgtattgg acgcgcagtt cccttacgcg gtccaggtgt
     6361 gaaacgatgt ccacgccgct tgcttgcgag cgggcatggt aattctcggt tacccgaatg
     6421 atcgtttcca ccacatttgg gaagcagccg caacagcgac cacgcttatg aatcgaatga
     6481 tagaccttgg ccgggacgat aagctgccac gggtcctggt cgagcagttc cacgattgcc
     6541 tgctcgactt ctttctgcgt tatgacgttg cactgacaaa tcagcatttt cgtctctttg
     6601 gctgacggag cgaggctccg tctggctaag cgttcttata cggtcggggc tgcccccggt
     6661 tcactggaaa accttgtccg gcgcgtcgag gctgtcggaa ccttcgaagg cgatggcgtc
     6721 gagcttcagc gccgaaacca cgcgttcgat ggatttggtc tggtcgacca gtccgtcgat
     6781 ggccgcctgc acggtggcgt tgccttcggt gttgccctcg gcgatctgct ggtcataggc
     6841 ttcgccgccc agcgcgcgcg ccttgatcgc ctccatcttg gagacggtca ggtcgagctt
     6901 gtccgacagt tccttgtcca gcgccggatc ggcggccttg accatgtcgg aaaccgatgg
     6961 gccggaaacg accgaaccgt cgatgcgcgt atagctggca tggtaggcgg cgcggatgcc
     7021 gacggcgtct tccagatgcg agatgtaggt gttgtcggag aagcagtcat gctcttcttc
     7081 cgggtcgtgc agcaacaggc cgagcttcat gcgctcgcct gccagttcgc cgtaggaaag
     7141 cgaacccatg ccggtcagga tggtggagat gccggcattc ggctcgccat cttcgagatt
     7201 cttgcgggcg gccccgcctt ccttccagtt gccgaccatt tcctgaagat ccgaaaccag
     7261 aagatcgctg gcggatttaa ggtagtcggc gcggcggtcg caattgccgc ccgtgcagtt
     7321 ggcggcgtca tagtcggtat agggccgctc gccggcgccc ggaccggtgc cgtgcaaatc
     7381 ctggccccag agcaggaatt cgatggcgtg atagccggtc gccacattgg cctcgatgtc
     7441 gcccgcttct tgcagcgtgc cggacaggaa ctccggcgtc agcttgctgg cgtcgatttc
     7501 cttgccgtcg atcttgatcg tcttgttggc gatgacattg gccgtgtaga gcgaattctg
     7561 gtccgactcg gtgccgtagc tcttggcgac gtaatcgatc aggccttcat ccagtggcca
     7621 ggcgttcacg cggccttccc agtcgtcgac gatagggttg ccgaagcggt agacctccgt
     7681 ctgctggtag gggatgcggg cggccttcca ggcctcgcgg gcagccgtca gcgtgtcggc
     7741 cgaaggcttg gcgatcagcg catcgacggc cttgtcgagc gcctgcgcgg tggtcagcga
     7801 atcctcgtat ttggcaagcg cgatgtcgga ataggtcttg atgaccgcct tggcgtcggt
     7861 gtcggccttc gccggcagca cgaaaacggc agccgtcagc gcggcggtgg cggccagtgc
     7921 ggcgaacctg ccggcatatc ggttgttcaa catcgacgtc tccttgagga tttcagcgaa
     7981 aaggggctgc ggccaagccg gcggcatggc agctatgggt cgggaaatga atggcgcggg
     8041 gatgcaaatt cgatgtcgcg cacatccgtg cgggtcgcaa catgcgccat gcggcgcgct
     8101 ctgggacgtt agacaccgaa atgcctccaa tcgaacgggt tcaaggccca tacatcaaag
     8161 gggtgcgcat ctgcgcgagg cttcataaaa cgacgggcgt gcaaaaagtc aactagtttg
     8221 atttgaaaaa agcaattgaa acaatagttt agaattattc taaactagaa cactcaactt
     8281 taagcgaggg cgggcggaac ggcgtggtcc cggcggcgat tgacaacggc ctgccccggt
     8341 gtgacgcgaa tgttgaaatt cacggcggaa ccgggctacc ccggaaccgg gggtcaccct
     8401 aggatgagcg acaggtcagg tgcagcatga atgatggcgt cgttattgtg ggtgcgggtc
     8461 acgcgggcgt gcaggcggca gccagcctgc gcgaggaggg ctatgacggc cccgtcactc
     8521 tcgtcggcga cgagaacgaa ctgccctatc acaagccgcc gctctccaag accttcatca
     8581 aggatgccca ggcaaagccg cagccgctgc gcggcgaggc cttctactcg ggcaacgcca
     8641 tcgatttccg tccgggcgta tcggtcgagc gcatcgatct tggccagcgg cggttgcagc
     8701 ttgccggtgg cggcgcgctg cctttcgacc ggctgatact ggcgacgggt tcgcgcccgc
     8761 gcccgctgcc gctgcccggt tccgacctgg ccggcgtgct ttcgctgcgc tccatcggcg
     8821 atgcgcggct gatccgcgac caaagcggtc gatgcacaga ggtcgtcatt ctcggcggcg
     8881 gcttcatcgg cctggaaatc gcggcgactt tgacggcggg cggccgcaag gtgacggtgg
     8941 tggaggcggc ggaccggctg cttggccgcg ccgtcgcgcc cgccatcggc gcccatgccc
     9001 gccagcggct ggaagacatc ggcgtgcgca tcctgaccgg cacgacgatt gcccggctcg
     9061 aaggcgaaaa cggccatgtc gttgcggcag tcacctcaac aggcgagcgt ctgccggcgg
     9121 aaatggtcat cgtcggcatc ggcgtggtgc ccaatgccga actggcgcaa gcggcgggta
     9181 tcgccaccgg caacggcatc aaggtggatg ccgcgatgca gacctcggtg ccgcaggttc
     9241 tggcgctggg cgacgccgcc agctaccgcc actggttcac cggcgccgac gtgcggctgg
     9301 aatcggtgca gaacgccacc gatcaggcgc gtcttgccgc ccgcaccatc accggcaagg
     9361 gcggcgccta tgcggcggtg ccgtggttct ggtcggacat tggcgacatg aaacttcaga
     9421 tggtcggcct gacatcgagc agcgaccgca atatcgtggt cggcgatccg gccgagaaca
     9481 agttttccat ctatcattat gccgacgacc ggctgatcgg catcgaatcc gtaaaccgcc
     9541 ccggcgacca catgctcggc cgcaagatgc tgggagccgg tttttcgcca ttgccggaaa
     9601 ccgtgtcgac cggcccggac ggcctgaagg cggcgctggc cgcatttcag caggaaacgc
     9661 aatagtctgt cgtcaaccgg tcggcgccac gcttacgcgc cggcatcgaa cgaacagggt
     9721 tcacgtcgcc acagcggggc ttcggctcgg tacgggcatt tcaggcctgc cggtcgcccg
     9781 ttgatgccag ccgtgcggcg gccgtcgccg ccattgcgtc ggccaggctc atattccatt
     9841 gcgctcggca ataagcgcgg aaatcgaagc agggcaggcc ggggcagacc tcgaattcaa
     9901 tcaggtggac ggtgtcgtcc gcctcgacgc ggaggtcgat ggagaagacg tcgcgcaggc
     9961 cgagcgattg catcagcgtt tgcgcgatcc ggcgaatttt catgtccgcg ccgggctggc
    10021 tggccttgac cggcacgagt tccggttcgg catagcggcc ttcggcccgc gccgcctcgc
    10081 cggttttgcc gtaaagggcg aggctgtctt ccatggtctg gaaatccgca ccggaatcga
    10141 cgaaggcgac gcccagcgcc tcgatgcccg cgttgcgctc caacccgagg aagctcgcac
    10201 gcacgttgcg gccggcgacg tagggctgca ccaccacctc gtcgcggtag gcggaaaaaa
    10261 tccgccggct aagctccaat gcatcggcaa ggtcgtggca gcgcgaatcc gcccagatgc
    10321 cgatcttggc gccgagccgg ttgggcttga cgaaccagcc ctgcgcagag gccggcggcg
    10381 tcaccagcca ttgcccgtta cgggcaaggc cggcagccgg cgccggcagg ccgagcgcgg
    10441 caagcaccgc gccggagcgg aacttgtcct ggcaaagcgc aaacagcgca tcgtcgctgc
    10501 caagcgtagg caggccgttc agccgcgcca aagcagggga agcgccgccg cgaaaatagg
    10561 cgatgccgtc ggtcagcgtc cacaccagcg tctttagcgg gtcgcgtctt gccagcgcct
    10621 ttgccgcatc gtccagatcg acgggcgtga aaacgatgtc gtgcctcgtg cacgccgccg
    10681 ccaacgcctc gaaatccggc gcgagatcgg ccgattgcgc cagataggac gaaatctcgg
    10741 ctgcgcgcgc aggggggtgg ccatcgccga caagccggtc gaagcaggcc ttttccgttt
    10801 cgtagacaag tatcaattgt gccggcactg attgtgccag ctttgattgg gaagatgaca
    10861 ttccggaatc ctgctgtgag tgagtggcta gagcagtccg ccgtcgacga tcagcgtctg
    10921 cgcggtcagc atcgccgagg cgtccgaagc gaggaacagg caaggcccga ccatgtcgtc
    10981 gggcagcatc atcctgggga tcgcctgccg tgaaatatgc cggtcgaggc tttcccgcgt
    11041 cacccacaat tcgttctggc gttcggtgat cacccatccg ggcgcaatgg cgttgacgcg
    11101 gatgccgtcc ttgccgtagc ggcctgcaag tcccttggtc aggccgacaa tgcctgcctt
    11161 cgccgccgta taggccggca tgtcgccaat attgagcatg aaggcggtcg aggtgaaatt
    11221 gacgatagag ccatgcccgg ccttgcgcat gcccggcgcc accgcctgca tggtgaagaa
    11281 atgcggccgc agattggttt cgaggttcgc gtcccagtcc tctggcgtca gttcctcggc
    11341 caggtggcgg tcgtcgcgcg ccgcgttatt gacgagaacg gtgatcggcc catgcgccgt
    11401 accggccttg gcgacgctgg cgcgcagcgc cgcgatatcg cgcaggtcgg cgtgcagata
    11461 gagcgggcga ttgcccgtcg ccgcctcgat ctcgtcgcac agcgccaggc tttgcttgtc
    11521 ggcaatgtcg atgaaggcga ccttcgagcc ctggcgcgcg aagccctccg tcaacctggc
    11581 accgatgccg gagccgccgc cggtgatcag caccgaagcg ccttcgagat cggcaaatcg
    11641 cgccgatggc atcatgaaat tttctccccc tctggccgcc gcgcatccta tccggtcaat
    11701 gccggcgggc aagggaaaac cccctgcgcg accgggctgc gccgccttgt cgttcgagat
    11761 ggtgtcaaag agcgctggtg cgcgacgccc cacgaaagct gtcgcgcaga tagccaagcg
    11821 tggcgttggc attgaccggc ttgccgaaca gatagccctg cccgccggcg cagccgaact
    11881 ggataaggcg gtcggcctgc gcttcctctt cgatgccttc agccaccaca tccatgccga
    11941 ggccctcgca catggcgagg atggcgcgga tgatgtgctc ggaggggcgg tcgtcgagga
    12001 tggaggagac gaaggcgcgg tcgatcttga gcttgtcgaa atggaattcg cgcagccgcc
    12061 caagcgagga ctggccggtg ccgaaatcat cgagcgagac gcggatgccg acgcggcgca
    12121 aatcttcgac ggttttgccc gccgaagacg ggtcgttcat cagcccggtc tcggtgatct
    12181 cgatttccag ccggcgcgga tcgaagccgg tgcggtcgag aatcgaaaga atatgcagcc
    12241 cggtgttctg atcgaccagt tgcgagggcg acagattgaa agcgaggaac aggtctttag
    12301 gccagttgcg cgccgcttct gtggccttgc gcaacaccag ttgcgacagc ggcccgataa
    12361 tgccgcgctc ttcggcaatg ggaatgaaca cggccggcga gaccgggccg aggtcgcggt
    12421 cggtccaccg ggccagcgtc tcgaaaccga tggtgcggcg gctgttgagg tcgacgatcg
    12481 gctggaaatg cggctcgacc tcgcctgccg aaacggcgcg gcgcaacgcc tgttcgatgc
    12541 gggtgacgcg cttggcggct tcttccattt cgcgggtata gacgacgacc tgcccgcggc
    12601 ccgaccgctt ggcgtggtag agcgccgttt cggccttgtc gatcaaaact tcggtggctt
    12661 cgtcaccgga ataaaacagc gagcagccga ccgacgccga cagccgcgcc gtgcgttccc
    12721 cgacgtcgta aggcgccgac aatatctcga tcagcatgcg cgcccgtttt gtcgcggctt
    12781 cttcactgaa caccatggga tagagaaagg cgaattcatc cgcgccgatc cggcagaccg
    12841 tcgaatgccc gtccatcgat gcgttgaggc gcatcgccac ctggaccaga atgtcgtcgc
    12901 cggccttatg cccgaacaga tcgttgatcg gcttgaagcc gtcgagatcg acgatgccga
    12961 cggcgaacgg cgccggatcc tcggatcggt cggaaatcag ccgttcgacc ttgtcaaaaa
    13021 agcgccgatg gttgccaagc ccggtcagcg gatcggtgaa tgccagatcc gatccttccc
    13081 tgcccgcctg cccggtgcca aacgaagttt gcatcggtgt ccctgttttt ataagtcgta
    13141 atttaccctg acaatggcag gaaactcttt aagaaaacgt attccccccg gtcgttagcc
    13201 ggcgtccggg gatcatgttc cggcagggcg gaaactatcc ttgcttgaca cggtaaagtt
    13261 gaagcggacc gtcctgcgcc ccggccaccg gctccagcca tgccggaatg gcgccgcgca
    13321 gcaactgcgc caggaagccg ccgggcgcgc cgtcgacgag cagtttgttt tccgggctgc
    13381 cggggcaaag cgcgaccagg ccgaccttgt tccggtcgat caccgtcttg gcttcggcaa
    13441 tcgtgcccat ataggcatcg agcacgagaa ggttgccggc gatgttgcga tggtaggggc
    13501 cagacagcgc ccgatgcccc gaataggcaa ggacaggcgc gcccagattg gaagttgcaa
    13561 gcacggtgga gtccggctgg gtggcgagaa tgctgaaatc cttgtctgcc ttgcagtcgg
    13621 ccatggccgg tttgccggca accggcgcgc ccgtcgcgga cggatcgtcc gcgatcatgg
    13681 ccgaggcgac ggtccagaag gcgttcatcg acaccagcca gacggcgatc atcttcagcg
    13741 aaaccgcggg cgtcggcgtg gccagcgcgc gctcgcgcca gctggcgatc cagccggaaa
    13801 gcgcgatgac ggccagcgcg atcgagaagg tcgagccgcg tacctgccag atgctgacga
    13861 cgaacgacgc gaccagcagc gcgccgacca ggctgtcctg ccgtcgccat gcgccttggc
    13921 gcagccgtgc cgccatgatg gcgatggcga ttatcggcgt gacatagcgc gccagcaccg
    13981 aactgtccct ggcctggatg agctggaaca tcgactgcgc ttcggaaata tgatcgagcc
    14041 aatattcgtg caaacgggga tcgaggccgg catagggtgc tgccaggcat tgcgggaacc
    14101 agtgaagcgt gatcgcggcc aggcctgcgc caagcaccgc cagcgccatc gcgcggcgca
    14161 taggggtgcg gcaggcggca tcgaaggagg ccgccaccgc aaggccggtg ccgcccagcg
    14221 ccgccagcac gaactggacg ctcgaaaagg cgtcgcagcg cgcctggccc caatcggcga
    14281 aagggatggt ggacacgaac accgtaagcg cggtgccggc gaagccaagt ccatattgcc
    14341 gggctacgga tcgctcgcct tggcctccaa acaggaacag gcctgccacg cacaggccga
    14401 tgacggccac gtaaggcgcg gtttccatgc cgatcgccaa tgtcagcgcg gcacagatgc
    14461 cggaaaatgc cgccgccgcc ttttgttggg gtgcggcgag caggaacgac aggctggcca
    14521 gcgtcagcat cagctggatg ttgtggtgat cgagggcgtt gggcgagaag atgccgagga
    14581 aatgcagcgc gccgccaccc gcgattacgg cgggcaggac ggcgctgtct ccgccgtagc
    14641 ggcgcgccgc gcgcgccagg aaaaaaacgc tcaggcaaag catcagcgcc ggccacagga
    14701 tgcgggcggc catttcgccg ccggcgatgc tgccggtaaa cgccgacgcg gcaaggatga
    14761 tggcggcgat cggcgcgtcg accagccgcg accagtgcat gacgaaaccg ccgtcgacgc
    14821 ccatcctgta ttggtgcagg tcgaaccagc cttgcccagc cagcaggtcg cgtatttcga
    14881 tcagccgaag caggctgtca ttgtccgcgg catcgtgcag ggttttgaag ccgctcatgg
    14941 catcgacggc aagcgcgatg agcgttgcga tgagcgcgag gcccagatcc tggctccagg
    15001 ccgcgcgcga agccctgaag gtcgttacgg cgctggagga catggcgcgc tggaagacat
    15061 ggacgacctt cggatcgatt gcgaagaccg accctagcct atgcggcttc aataaagcgt
    15121 aaagttcgct tcgccctagc gccggtcggc aaacacccag aaggtggaaa gcacataact
    15181 ggcggcgctt gccgcaagcg tggtgaatgc cgacatggca agcggcgcca tgccgaaccg
    15241 ggtcaccgcg acatgagcga gcacgccgga aaacaatgcg cagcccagtt gcagcgtcag
    15301 gtagcggggc agcacctggc catggtcgtg cctgccttcg aaggtccagg agcgctgtgc
    15361 gcaataggcg acgcagaaag ccgagccata ggcaatggcg ctgccgatga acggaggtac
    15421 gccgaacgat accaatagcc atgaaaggcc gaaaaacagt gccgccgcgc caacgcccac
    15481 cacgacgaac cggaccagtc gctggccccc ggcgccatga aggattgcgc ccatcgcttg
    15541 gttaaagcgc ggaatcggca cggcccgtac cggcggctgc cagccgcttg agcggaattt
    15601 ccaatacgcc aagcagaaag gcggagaaga agagttgtag cgaaatgacg atcagcgtca
    15661 gtcccatgat gacgacgcgc gggatttcgg aattattcag cgggccgaaa ccgacatgcg
    15721 cccattgcag cacggcgtag ccgaaaaaca gcgcaccacc gaagaagcag atgccggcat
    15781 cgatggcgag gctttcggtg ctgatggtgc cggacagcca gttggaccgg cgcgtgctgg
    15841 gcagcatctg ggtcttctcg gcaaagcagc gcgagatcac gccgaaagtc agcaactgca
    15901 ccccggtgag aatcatgaag caggcggcgg tgaacgtgtt cagatcgagt gacatattgt
    15961 cgatcacgcg caatggccca aacagcagca gcgcggcaaa caccgcgccg acggcgcaca
    16021 ggaaggcgcc tggaacgaag aacagccagc gcggattgta caccagcagg aatttcaggt
    16081 gacgccagcc gtcgcgccac gttctgaggt gcggtggccg gctgcgcccg tccggcttga
    16141 gggttgtcgg cacttcctcg atccgcaaat tggccagcgc gctgcgcacc accatttcgc
    16201 tggcgaattc catgccggtc gtctgcaaat ccagcttgcg gatggcttcg gtgttgaagc
    16261 cgcgaaggcc gcagtggaag tcgccggcct tgatgcggaa gaacaggcgg ccgatgaagc
    16321 ttagaaccgg attgcccaga tagcggtgca aaggcggcat ggcgccgggt tcgatgccgc
    16381 cctggaagcg gttgcccatg acgaggtcgg caccctcgcg cagccgcgcc acgaacgcgc
    16441 ccagcgcgga aaaatcatag ctgtcgtcgg cgtcgcccat gatgacgaag cggcctctgg
    16501 cgctggctat gccgcccaga agggccgcgc catagccctt ttgcgcgacg ggaacgacac
    16561 gcgcgcccag cgccgtcgca atggcctgcg agccatcggt gctgccattg tcggcgatca
    16621 ggatttcacc aataatcccg gaagcatcga gaaatgccct ggccttgttg atgcacacgg
    16681 ccagcgtctc ggcctcattg aggcaaggca tcaggagcgt cagttccatt tgccgggttg
    16741 cggcggaacc ggtgggcgtg actgcgttca tcatcgcaac tttccagaca tccgccggtg
    16801 cacgaaaatg tcggcggcta ttggccgaac gcttacacgg caagccttaa gaaaccgatg
    16861 acgcgcatgc gcgaactgtt ggcgatcctg ccgccgatcc gatcgtcaaa aagacgtgat
    16921 tggcagcgca aggccaagcc tgtacaggca agggcaacgc ttcaatcccc atgccggaga
    16981 taaatccatg tccatgaaag tcgccgtcca gatggaccac gtttccaccg tctcgatcat
    17041 gggcgacacc tcgttcgcac tatcgctgga agcgcaacgg cgcgggcacc ggcttttcca
    17101 ttatacgccg gaccggcttt cgatgcgcga cggcaaggtg tttgcccgta tcgaggaaat
    17161 gcagctgcgt gacgagaagg ccaaccacta tacgctgggc gaaccggtgc gcaccgacct
    17221 gtctgaaatg gacgtgatcc tgctgcgcca ggacccgccc ttcgacatga actacatcac
    17281 caccacccac atgctggagc gcatccaccc gaagacgctg gtggtcaacg acccggcctg
    17341 ggtgcgcaac agccccgaaa agatattcgt caccgaattt cccgacctga tgccggaaac
    17401 gctgatcacc aaggacccgc gcgaggtcga ggccttccgc aaggagttcg gcgacatcat
    17461 catcaagccg ctttacggca atggcggcgc cggcgtcttc cagctcaatg acggcgaccg
    17521 caacctgacc tcgctgctgg aaatgttctc gcagatgttc cgcgagccgt tcatcgtgca
    17581 acgctacctc aaggacgtgc gcaagggcga caagcgcatc atcctcatcg atggcgagcc
    17641 tgtcggtgct gtcaaccggg tgccgtcgga aacggaatcg cgctccaaca tgcatgtcgg
    17701 cggtcgcgcc gaaaagagcg agttgaccga ccgcgagcgc gagatttgcg cacgcatcgc
    17761 gccgtcgttg aaagcgcggg gcttcatcct cgtcggcatc gacgtcatcg gcgactacat
    17821 gacggaaatc aacgtcacct cgccgaccgg catccgcgag atcaagcgtt ttggcggcgc
    17881 agatatcgcc ggcctgttct gggacgccgt cgaggacaag cgccgcagct aggcgc
//
LOCUS       DASYCS010000116         2243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1909367,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000116 DASYCS010000000
VERSION     DASYCS010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1909367"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..903)
                     /gene="cysW"
                     /locus_tag="VGM46_04115"
     CDS             complement(22..903)
                     /gene="cysW"
                     /locus_tag="VGM46_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HEY4191810.1"
                     /translation="MADPVLFAANPEPENASGITEGRPARYALMAVAFVFLAIFLLMP
                     LVLVFIQAFAKGAGYFLSTLGEPDTLSAIRLTLIVAAIAVPLNLVFGVAMAWAIAKFE
                     FKGKAFLTTLVDLPFSVSPVISGLVYVLLFGAQSLLGPWLKAHGITILFAVPGIVLAT
                     VFVTFPFVARELIPLMQEQGTKDEEAAISLGANGWQTFRYVTLPNIKWGLLYGVLLCN
                     ARAMGEFGAVSVVSGHIRGQTNTMPLHVEILYNDYNAAGAFAVASLLAGLALVTLALK
                     TLLEMRYGAEIAATRGH"
     gene            complement(896..1747)
                     /gene="cysT"
                     /locus_tag="VGM46_04120"
     CDS             complement(896..1747)
                     /gene="cysT"
                     /locus_tag="VGM46_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019859195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HEY4191811.1"
                     /translation="MTIAPAPAGWRFKKPSVIPGFGLTLGLSLAYLTLIILIPLSGLV
                     WRSAALGWHDFWAIATDPRTLNSLKISFGTALVAAIVNVVFGTLVAWVLVRYSFPGRR
                     IIDAMVDLPFALPTAVAGIALTTLYAPNGWIGSLLAPLGVKVAYTPLGIIIALVFIGL
                     PFVVRTVQPIMEEIDKEVEEAAATLGASRFQTITRVLFPGLAPAILTGFALAFARGVG
                     EYGSVIFIAGNLPNVSEITPLLIVIRLEEFNYPAATAIAAIMLIISFAMLLVVNLLQS
                     WSRKRYG"
     gene            complement(1788..>2243)
                     /locus_tag="VGM46_04125"
     CDS             complement(1788..>2243)
                     /locus_tag="VGM46_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019859193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0043199 - sulfate binding [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY4191812.1"
                     /translation="ANVPVLDTGARGSTVTFAQKGLGDVLLAWENEAYLALDEFGADN
                     FDIVYPPTSILAEPPVAVVDGNVDQKGTRKVAEEYLKYLYSKEGQTIIARNHYRPSKP
                     ELVPAEDLAKQPAIKLITIDDPLFGGWKKAQPYHFGDGGIFDQIYKPAQ"
BASE COUNT          471 a          643 c          751 g          378 t
ORIGIN      
        1 aacttccatg aaaaagacct ctcaatgtcc gcgcgttgcg gcgatttccg cgccgtagcg
       61 catttcaagc agggttttca acgccaaggt caccagcgcc agcccggcca gcagcgaagc
      121 cacggcgaag gcgccggccg cgttatagtc gttgtagaga atttccacat gcagcggcat
      181 ggtgttggtc tggccgcgta tgtggccgga gacgaccgaa accgcgccga actcgcccat
      241 ggcgcgcgca ttgcacaaca aaacgccgta gagcaggccc catttgatgt tgggcaaggt
      301 tacgtaccgg aaggtctgcc agccattggc gccgagcgag atggccgctt cctcgtcctt
      361 cgtgccttgc tcttgcatca gcgggatcag ttcgcgcgcg acgaacggga aggtgacgaa
      421 gacggttgca agaacgatgc cgggcacggc aaacaggatg gtgatgccat gcgccttcag
      481 ccacgggcca agcaggctct gcgcgccgaa caaaagtacg tagacgaggc cggaaatgac
      541 cggcgacacc gagaaaggca agtcgaccag cgtggttagg aaggccttgc ccttgaattc
      601 gaacttggcg atcgcccagg ccatggcgac gccgaagacg aggttgagcg gcacggcgat
      661 ggccgccacg atgagcgtca gccggatggc ggagagcgta tcgggttcac ccagcgtcga
      721 caggaaatag ccggcgccct tggcgaaggc ctggatgaaa accagcacca acggcatcag
      781 caggaatatg gcgagaaaca cgaaggcgac cgccatcagc gcataacggg cggggcgccc
      841 ctcggtgatg ccggaagcgt tttccggctc tgggttggcg gcgaagagca cgggatcagc
      901 catagcgctt gcggctccac gactggagga ggttgacgac cagcagcatg gcgaacgaga
      961 tgatcagcat gatggcggcg atggccgtgg cggccggata attgaattct tcgagccgga
     1021 tgacgatgag aagcggcgtg atttcggaaa cgttcggcag gttgccggcg atgaagatga
     1081 cggagccata ttcgccgacg ccgcgcgcga aggcgagcgc gaagccggtc aggatcgccg
     1141 gagccagccc cggaaacagc acgcgggtga tggtctggaa gcggctggcg ccgagcgtcg
     1201 cggccgcttc ctcgacctcc ttgtcgatct cttccatgat cggctgcacg gtgcgcacga
     1261 caaaaggcag cccgatgaac accagcgcga tgatgatgcc gagcggcgta taggcgacct
     1321 tgacgccaag cggcgcaagc agcgatccta tccagccatt aggcgcataa agcgtggtca
     1381 gcgcaatgcc ggcaactgcg gtcggcagcg caaacggcag gtcgaccatc gcgtcgatga
     1441 tgcggcggcc aggaaagctg tagcgcacca gcacccaggc gacgagggtg ccgaaaacga
     1501 cgttgacgat ggccgcgacc agcgccgtgc cgaagctgat cttcagggaa ttcaaggtgc
     1561 gcgggtcggt ggcgatggcc cagaagtcat gccagccaag ggcggccgaa cgccatacca
     1621 gccccgatag cgggatcagg atgatgaggg tgaggtaggc aagcgagagg ccgagcgtca
     1681 atccaaaacc tggaatgacg ctcggcttct tgaatcgcca ccccgctggt gcgggtgcta
     1741 tggtcatgtc gtccttatcg cgtggtccca atccatgggt ctattggcta ctgggccggc
     1801 ttgtagattt ggtcgaatat accaccgtcg ccgaaatggt aaggctgcgc cttcttccat
     1861 ccgccgaaaa gtggatcgtc gatggtgatc agcttgatgg cgggctgctt ggcgaggtct
     1921 tcggccggca ccagttcggg cttggaaggc cggtagtggt tccgggcgat gatggtctgg
     1981 ccctccttgg aatagagata tttcagatat tcctcggcca ccttgcgggt gcctttttga
     2041 tcgacattgc cgtcaaccac cgcgacgggt ggttcggcaa ggatcgaggt cggcgggtag
     2101 acgatgtcga aattgtccgc gccgaactcg tcaagcgcca gataggcttc gttctcccag
     2161 gccagcagca cgtcgcccag gcccttttgc gcgaaggtca ccgtcgagcc gcgcgcgccg
     2221 gtgtcgagca cgggcacgtt ggc
//
LOCUS       DASYCS010000117         1840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_749854,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000117 DASYCS010000000
VERSION     DASYCS010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749854"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..125)
                     /locus_tag="VGM46_04130"
     CDS             complement(<1..125)
                     /locus_tag="VGM46_04130"
                     /inference="COORDINATES: protein motif:HMM:NF025034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HEY4191813.1"
                     /translation="MIGGAAIRAVVDTGASSTVLSADDAEAAGLDPDSLKYTVPVA"
     gene            108..422
                     /locus_tag="VGM46_04135"
                     /pseudo
     CDS             108..422
                     /locus_tag="VGM46_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909483.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
     gene            complement(427..1131)
                     /locus_tag="VGM46_04140"
     CDS             complement(427..1131)
                     /locus_tag="VGM46_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006094275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HEY4191814.1"
                     /translation="MNRFFWIIMVAIGVALALLIFNSAGGKTLGMANDDFGQLVWLGI
                     FAAVIGAGVLRAGGPVGTLARQFAAWAAIVLALIAGYQYRYELQDFASRVSAGLVPGS
                     PLALGEADGRATVTLEKSLSGHFEARLIVNGAAIRAVVDTGASSTVLSADDAEAAGLD
                     PDSLKYTVPVATANGGTNAAGARTKELSIGGIVRMNMPVLVAAPGTLEQSLLGMNFIG
                     TLSGFDVRGDRMILRD"
     gene            complement(1145..1378)
                     /locus_tag="VGM46_04145"
     CDS             complement(1145..1378)
                     /locus_tag="VGM46_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HEY4191815.1"
                     /translation="MTAIETPCILVCSIDRTTGYCFGCGRTQAEIGAWIEMTPDTRRS
                     VMAELPARLETVERKPRRETRRTRLARERDSTV"
     gene            1539..>1840
                     /locus_tag="VGM46_04150"
     CDS             1539..>1840
                     /locus_tag="VGM46_04150"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006333687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HEY4191816.1"
                     /translation="MNSALPFDDFRDLLANLPAADSQAQARVRTLFAKANKAQGSLGR
                     IEDIAAWLATWSGRAPPAIHRPLVAIFAGNHGVARHGISPRPTSATADAVELCAAGG"
BASE COUNT          337 a          613 c          574 g          316 t
ORIGIN      
        1 gccaccggca cggtgtattt caggctgtcc gggtcgaggc cggcggcttc ggcatcatcg
       61 gcgctcaaca ccgtgctact cgcgccggta tcaaccaccg cgcgaatggc cgcgccgcct
      121 atcatacgcc gggcatgctg acggccgtcg atgcggccgt ctatggcgag ccgcggcgcg
      181 atttcgacta tcctcaactg atcgaaacca tggcggccta cgcaacaccc catatcgaga
      241 agggcggccg tcttggccat gtcacccgcc acatggtcgg gttgtttcat gggctggcgg
      301 gcgctcgccg ttggcggcaa attctctcca ccgacgcgac acgtccgggg gcaggcgctg
      361 aagtactgaa agcggccttc gcggcagtcg acctcgacgg ggcggcggag caggctgcct
      421 gacggatcag tctcgcagga tcatccggtc gccgcgcacg tcgaagccgg acaacgtgcc
      481 gatgaaattc atgccgagca ggctttgctc aagcgttccc ggcgcggcta ccagcaccgg
      541 catgttcatg cgtacgatgc cgccgatgga caattccttg gtgcgtgcgc cggccgcgtt
      601 cgtcccgcca ttcgccgtag ccaccggcac ggtgtatttc aggctgtccg ggtcgaggcc
      661 ggcggcttcg gcatcatcgg cgctcaacac cgtgctactc gcgccggtat caaccaccgc
      721 gcgaatggcc gcgccgttga cgatgaggcg tgcttcgaaa tgtccgctca gcgacttttc
      781 aagcgtcacc gtggcacggc catcggcttc gccaagcgcc aacggacttc caggaacgag
      841 accggcactg acacggctgg cgaagtcctg caattcatag cggtattggt agccggctat
      901 caacgccaga acgatggctg cccatgccgc aaactgcctt gccagcgtgc cgacagggcc
      961 gccggcgcgc agcacgccgg cgccgatcac ggcggcgaaa atacccagcc agacaagctg
     1021 tccaaagtcg tcattggcca tgccgagggt ttttccgccg gcgctgttga agatcaacag
     1081 agccagcgcg acaccgatgg caaccatgat gatccagaag aagcggttca tggcgggcga
     1141 ccgttcacac agtcgaatcg cgttcgcgcg ccaggcgcgt acggcgcgtt tcccggcgcg
     1201 gtttgcgttc caccgtttcc agccgggccg gcagttcggc catgacggaa cgacgggtgt
     1261 cgggcgtcat ctcgatccat gcaccgattt cggcctgcgt gcgtccgcaa ccgaagcaat
     1321 agccggtcgt cctgtcgatc gagcaaacca gaatgcaggg cgtttcgatg gccgtcatag
     1381 aagaaatccc ttggcttgtt tccacatggt gcaacggaag cgccaatgca agccccgccg
     1441 aaaccgccgc cgccggtcgc ttcaacgtca aagccgggag gttgtgccga cgcatcaaca
     1501 ggtctatccc gctgccatca ccacggacgg ttattcatat gaacagtgcc ctgcctttcg
     1561 acgacttccg cgatcttctc gccaatctgc cggccgccga cagccaggcg caggcaaggg
     1621 tgcgaacgct gtttgccaag gccaacaagg cgcaagggtc gcttggccgg atcgaggata
     1681 tagcggcatg gctcgccaca tggagcgggc gcgcgccgcc cgccatccat cgcccgctgg
     1741 tagcgatctt tgccggcaac cacggcgttg cccgccatgg catttcgccg cgcccgacaa
     1801 gcgccacggc ggatgcagtg gaactgtgcg cggcaggcgg
//
LOCUS       DASYCS010000118         7111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2780325,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000118 DASYCS010000000
VERSION     DASYCS010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 7111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7111
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2780325"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="VGM46_04155"
     CDS             <1..269
                     /locus_tag="VGM46_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY4191817.1"
                     /translation="MGDLRAMGETNALIDRPRRPGTRALFARAAEIYAQRFSDADGRI
                     RASFSLVWMSGWAPDPSQRKPLRPGSAKVSLASILEAPARREAE"
     gene            complement(297..488)
                     /locus_tag="VGM46_04160"
     CDS             complement(297..488)
                     /locus_tag="VGM46_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEY4191818.1"
                     /translation="MKILIRRFCQARFFQDRRGATAIEYGLIAALLSLAIFAGFGNVA
                     DRLEWLWSDNSSRLVQGLN"
     gene            complement(607..921)
                     /locus_tag="VGM46_04165"
     CDS             complement(607..921)
                     /locus_tag="VGM46_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191819.1"
                     /translation="MSLDEDWDSIRPQRGFRAFNAGMGVLRIALLFGSAAIALALIAV
                     PLLDNDNGRQIARDSFPGIDTTTTGSIGHSGTYTVRRSVLQASPESVCLIRANGRRSG
                     DC"
     gene            complement(1035..1451)
                     /gene="mutT"
                     /locus_tag="VGM46_04170"
     CDS             complement(1035..1451)
                     /gene="mutT"
                     /locus_tag="VGM46_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010657503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HEY4191820.1"
                     /translation="MQEGTVKRLLLVAACALVDADGRVLLAQRPPGKQLAGLWEFPGG
                     KVEAGETPEACVVRELREEIGVETKVACLAPLTFASHSYDDFHLLMPLYVCRRFEGIA
                     VPKEGQTLKWVRPRQMRDYPMPPADAPLIPFLIDLL"
     gene            complement(1454..2203)
                     /locus_tag="VGM46_04175"
     CDS             complement(1454..2203)
                     /locus_tag="VGM46_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006328559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY4191821.1"
                     /translation="MLAIVRRYEAAGFRAWPAAAVHYDGTWVVRLTAGHPAKRLNSVN
                     PLDPGDIGQIPERIGRASRRFEAYGRPLTFRLSPLSGSVIPAHLDAQGWSRFDESLVM
                     RLPLADAAVADAMDQLPLRDISRFVGASLKVHDLDVSLRAGLSEIIGAIQPEAGLFVM
                     ERNDEPLATLICVHDGDLAGLFEIATDKAFRNQGHGRQLILSALKWARLRGAREAWLQ
                     VEADNHAALGLYRALGFTEVYRYHYRRPQGA"
     gene            complement(2233..3474)
                     /gene="argJ"
                     /locus_tag="VGM46_04180"
     CDS             complement(2233..3474)
                     /gene="argJ"
                     /locus_tag="VGM46_04180"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF003802.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEY4191822.1"
                     /translation="MSDTISPLAPRKYPKMPSIEGVRIATAEAGIKYKNRTDLLVMAF
                     DAGTTVAGVFTRSKCPSAPVDFCRQNLAQGKARILVVNSGNANAFTGKKGRETTTVTG
                     EAAAKAANCMPGDVFLASTGVIGEPLDAGKFGPLLAGMVKDSKPDLWLDAAKAIMTTD
                     TYPKVATATVKLGDADVTLNGISKGAGMIAPDMATMLSFIATDAPIAAPVLQDLLSRG
                     VGKTFNAVTVDSDTSTSDTLLLFATAAAARRGAATITDPKDPRLGAFRRALGKMLKSL
                     ALQVVRDGEGARKQIEVTVTGAKSAKSAKRIALSIANSPLVKTAVAGEDANWGRVVMA
                     VGKAGEPADRDRLSIWFGDNRLAHEGERDMAYSEAATSAYMKRDEIRIRADIGIGGGK
                     ATVWTCDLTKEYVAINGDYRS"
     gene            complement(3659..3735)
                     /locus_tag="VGM46_04185"
     tRNA            complement(3659..3735)
                     /locus_tag="VGM46_04185"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3699..3701),aa:Arg,seq:tcg)
     gene            complement(3841..4755)
                     /locus_tag="VGM46_04190"
     CDS             complement(3841..4755)
                     /locus_tag="VGM46_04190"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY4191823.1"
                     /translation="MSLLFSRSPFAGLGFVLGLTVLSLSPALAQDAAPAAPAPAAAAA
                     PVDPAAVVATVNGKPVTEADLTLAEGELSQQFAQLPPEQRRAAALSAAIEIKVLAAKA
                     TADGLDKDPDFQRRIAFLQERALHGVMVEKEVVGKVTDDEIHARYDKEIAAAPPVNEV
                     HARHILVKTKEEAEAIIKQLDGGADFQKLANEQTSDPSGKTNGGDLGYFGPGQMVPEF
                     DKAAQALEVGSYTKEPVQTQFGWHVIKVEDKRAKQPPAFDTVKDNVRSLIIRDKYFAL
                     VKSLRAAAKVEIPDAALKKAVDAAEGGE"
     gene            5009..>7111
                     /gene="secA"
                     /locus_tag="VGM46_04195"
     CDS             5009..>7111
                     /gene="secA"
                     /locus_tag="VGM46_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006199910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEY4191824.1"
                     /translation="MVSFGGLARKVFGSSNDRRVKATRPRLEAINAMENEMRALSDAD
                     LAARTVKFREDLANGATLDDLLVPAFATVREAARRVLGLRPFDVQLIGGMVLHDGGIA
                     EMRTGEGKTLVATAPVYLNALAQKGVHVVTVNDYLASRDAEWMGRVYKFLGLSIGIIV
                     HGLDDEERSAAYACDVTYATNNELGFDYLRDNMKYERSQMVQRGHNYAIVDEVDSILI
                     DEARTPLIISGPLEDRSEMYNTIDTFMLRLSPADYEIDEKQKTSIFTEEGTEKLENML
                     RDADLLKGESLYDVENVAIVHHVNNALKAHQLFQRDKDYIVRNGEIVIIDEFTGRMMP
                     GRRYSEGLHQALEAKEHVQIQPENQTLASVTFQNYFRLYKKLAGMTGTALTEAEEFGN
                     IYGLEVTEIPTNVPVQRIDEDDEVYRTVEEKYKAIVREIKEAHAKGQPTLVGTTSIEK
                     SEQLAERLRKDGFKDFEVLNARHHEREASIVAQAGKPGSITIATNMAGRGTDIQLGGN
                     ADMRIDEELADVPEGPERDAREKAIREDVQRLKDKALAAGGLYVLATERHESRRIDNQ
                     LRGRSGRQGDPGRSKFFLSLQDDLMRIFGSERMDGMLQKLGLKEDEAIIHPWINKALE
                     KAQTKVEARNFDIRKNLLKYDDVSNDQRKVVFEQRIELMDGEGLTEIIAEMREGVIDE
                     IVAKAIPENAYAEQWNVAG"
BASE COUNT         1390 a         2294 c         2203 g         1224 t
ORIGIN      
        1 tcatgggcga tctgcgggcg atgggtgaaa ccaacgcgct gattgaccgg ccgcgccgcc
       61 cgggcacgcg cgcgcttttc gcccgcgccg ccgaaatcta cgcgcaacgc ttttcagacg
      121 ccgacggacg catcagggca agcttttctc ttgtctggat gtccggctgg gcgcccgacc
      181 cgtcgcagcg caagccgctc cggccgggtt cggcgaaagt ttccctggcc tcgatattgg
      241 aagcgcccgc acgccgcgaa gcggaataag acgcagcaat cagaggcaag ccaggcctaa
      301 ttcaggccct gcacgaggcg gctactattg tcggaccaca gccattcgag cctgtctgcc
      361 acattgccga agccagcgaa aatggccaac gacagcaacg ccgcgatcaa gccatattcg
      421 atggccgtcg ccccgcggcg atcctgaaaa aaccgggcct ggcaaaaccg gcgaatcaaa
      481 attttcatgg ccgtcgatcc cctcgccgga tcattggtag gcaagacgtg ttaaggaaag
      541 ttaacggcgc aagcttaaca ggccataaac cggcaagctg cgcgatatca gccgggctga
      601 tcgccctcag caatcgccgc tgcgccggcc gttggcgcgg atgaggcaca ccgattcggg
      661 cgacgcctgc aacacgctgc ggcggaccgt ataggtgccg gaatggccga tcgatccggt
      721 ggtggtggta tcgatgccgg gaaagctgtc gcgcgcaatc tggcgaccat tgtcattgtc
      781 gagcagcggc acggcgataa gcgccagcgc gattgccgcc gagccgaaca gcagcgcgat
      841 ccgcaaaacg cccatgccgg cgttgaaagc ccgaaaccct cgttgcggcc ggatcgaatc
      901 ccagtcttcg tcgaggctca tgcagccatt ccccagcaaa tccgctctgc gcaaaagcgc
      961 cgtgttgcct caatttttca cagcaacact aatcttccat taacgatgcc gaatgcggcc
     1021 aaaaactgcc tgaatcacaa cagatcgatc aggaatggaa taagtggggc atccgccggc
     1081 ggcatcggat aatcgcgcat ctggcgcggc ctcacccatt tcagcgtctg gccttccttc
     1141 ggcaccgcaa tgccctcgaa gcggcggcag acgtaaagcg gcatcaaaag atggaaatca
     1201 tcatagctgt gactggcgaa ggtaagcggc gcaaggcacg cgaccttggt ttcaacgccg
     1261 atttcctcgc gtaactcgcg caccacgcag gcttcgggcg tttcgccggc ctcgaccttg
     1321 ccgccgggaa actcccataa accggcaagc tgcttgcccg gcggccgctg cgccagaagc
     1381 acgcgcccat ccgcatcgac cagcgcgcag gcggcgacca gaagcaggcg cttcaccgtg
     1441 ccctcctgca ccgtcaggcg ccttgcggcc ggcgataatg gtagcggtag acttccgtga
     1501 agccgagcgc ccggtaaagc cccagcgcgg catggttgtc ggcttccacc tgtagccacg
     1561 cctcgcgcgc cccgcgcagg cgcgcccact tcagcgccga aaggatcagt tgccgtccat
     1621 ggccctgatt gcgaaacgcc ttgtcggttg caatctcgaa caggccggca aggtcgccgt
     1681 cgtgaacgca gatcagcgtc gccagcggct cgtcattgcg ctccatcacg aacaggccgg
     1741 cttccggctg tatggcgccg atgatttcgg acaggcccgc ccgaagcgaa acatccagat
     1801 catggacttt gagcgaagcg ccgacaaagc ggctgatatc gcgcaacggc aactggtcca
     1861 tcgcgtcggc gaccgccgca tcggccagcg gcaggcgcat caccaaagat tcgtcgaaac
     1921 ggctccaccc ttgtgcgtcg agatgcgccg ggatgacgga gccggaaagc ggcgacaagc
     1981 gaaacgtcaa cgggcgtccg taagcctcga agcgccggct ggcgcgcccg atgcgctccg
     2041 gaatctgccc gatgtcgccc ggatcaagcg ggttgaccga attcagccgc tttgccgggt
     2101 ggccggccgt cagccgcacc acccaggtgc cgtcataatg cacggccgcc gccggccatg
     2161 cgcgaaaccc cgccgcctca tagcggcgca cgatggcgag catggttgcg gggtgcctgg
     2221 agtccaagac ggtcagctcc ggtagtcgcc gttgatggcg acatattcct tggtgaggtc
     2281 gcaggtccac accgtcgcct tgccgccgcc aatgccgata tcggcgcgaa tcctgatttc
     2341 gtcgcgcttc atataggccg aggtagccgc ctcggaataa gccatgtcgc gctcgccttc
     2401 atgcgcaagg cgattatcgc cgaaccagat cgacaggcgg tcgcggtccg ccggctcgcc
     2461 cgccttgccg acagccatga cgacgcggcc ccaattggcg tcctcgcccg ctacagccgt
     2521 cttgaccagc ggcgaattgg cgatcgacag cgcgatgcgc ttggccgatt tcgccgattt
     2581 cgcgccggtg accgtgacct cgatctgctt gcgcgcgccc tcgccgtcgc gcaccacctg
     2641 caatgccagc gacttcagca ttttgcccag cgcccgccga aacgcgccaa ggcgcgggtc
     2701 tttcggatcg gtgatcgtcg ccgcgccgcg acgggcggcg gcggcggtgg cgaacagaag
     2761 cagcgtgtcg ctggtcgagg tgtcgctgtc gaccgtcacg gcattgaagg tcttgccgac
     2821 gccgcgcgac agcaaatcct gcaagaccgg cgctgcgatc ggcgcgtcgg tggcgatgaa
     2881 ggacagcatc gtcgccatgt cgggcgcgat catgccggcc cctttggaga tgccgttgag
     2941 ggtgacgtcc gcatcgccga gcttcaccgt cgcggtcgcc accttgggat aggtgtcggt
     3001 cgtcatgatg gccttggccg cgtcgagcca cagatcgggc ttgctgtcct tgaccatgcc
     3061 ggccagcagc gggccgaatt tgccggcgtc gagcggctcg cctatcaccc ccgtcgaggc
     3121 gaggaacacg tcgcccggca tacaattggc ggcctttgct gcggcttcgc ccgtgacggt
     3181 ggttgtttcg cggccttttt tgccggtaaa ggcattggcg ttgcccgaat tgacgacaag
     3241 gatgcgcgcc ttgccctgcg ccagattctg gcggcagaaa tcgaccggag ccgacgggca
     3301 tttcgaccgc gtgaacaccc ccgccaccgt cgttccggca tcgaacgcca tgaccagcag
     3361 atcggtgcgg ttcttgtact tgattccggc ttccgccgtg gcgatgcgca cgccctcgat
     3421 cgaaggcatt ttgggatatt tcctgggagc gagcggcgaa atcgtgtcgg acatgggagc
     3481 ctcggcgaaa aatgcgaaac gaggccggtt tgcccgatgc cgcgccctag cgcaaggcgt
     3541 ttggtgtgac ccggcaccaa gacgaacccc ctgggttctc gagggcaacc gcccgtcggc
     3601 catcaggccg ccccatcgga gccgtgagcg aagcgaattg gcgtgagata agaaaaagtg
     3661 gcgcgctcgg caggatttga acctgcaacc tacggattcg aagtccggtg ctctatccag
     3721 ttgagctacg agcgcttgaa ccgggattga tcggccaacc tcgatggcgc gtgacaaggc
     3781 gagtagaata gaaaaaggcg gacttcgaaa agcccgcctt ttgcagaaaa tcagggccgc
     3841 ttattcgccg ccttccgccg catcgaccgc cttcttgagc gcggcgtcgg ggatttccac
     3901 cttggcggcg gcacgcagcg acttgaccag cgcgaaatat ttgtcgcgga tgatgagcga
     3961 acggacattg tccttcaccg tatcgaaggc cggcggctgc ttggcgcgct tgtcttccac
     4021 cttgatgaca tgccagccga actgggtctg caccggctcc ttggtgtagc tgccgacttc
     4081 cagcgcctgc gccgccttgt cgaattccgg caccatctgg ccggggccga aatagccgag
     4141 gtcgccgcca ttggtcttgc cgctcggatc gctggtctgc tcgttggcca gtttctggaa
     4201 gtccgcgccg ccatccagtt gcttgatgat ggcctcggct tcttccttcg tcttcacgag
     4261 aatgtgacgg gcatgtacct cattgacggg cggtgcggcg gcaatttcct tgtcgtagcg
     4321 ggcatggatc tcgtcatccg taaccttgcc gacgacttcc ttttccacca tcacgccatg
     4381 caaggcgcgc tcctgcaaga aggcgatgcg gcgctggaag tccgggtcct tgtcgaggcc
     4441 gtcggccgtc gccttggcgg ccagcacctt gatttcgatg gcggcggaaa gcgcggcggc
     4501 acggcgctgt tccggcggaa gctgcgcgaa ctgctgcgaa agctcgcctt cggccagcgt
     4561 caggtcggct tcggtcaccg gcttgccgtt gacggtggcg accacggcgg ccggatcgac
     4621 gggcgccgcg gcggcggcag gcgcgggcgc ggctggcgct gcatcctggg ccagggccgg
     4681 ggacagggac aaaacggtaa ggccgagaac gaatccgagg ccggcaaacg gcgagcggct
     4741 aaacagcaag gacatgaaat aatgactcca gggggttggc ttgagcgggg gacaggcttc
     4801 tcagatcaac ctgaatgtgg cggaatttgg cgcgatagcc atgcctcgcg cgccgttgac
     4861 atcgattgaa ccccctctta tctgtccagc gactttgcgt ccagaaccgt tttccgggcg
     4921 cttttgcgcg tttcgtccgc ctgtcgcgga tcagacgggg ctggcaggca ccgtcttgtt
     4981 gatcaggatt gcaacgaaag gaccatttat ggtcagtttt ggcggcctcg cccgtaaggt
     5041 tttcggctct tccaacgacc gccgcgtcaa ggcgacccgg ccaaggctgg aagccatcaa
     5101 tgccatggaa aacgagatgc gcgcgctgtc cgacgccgat ctcgccgcgc gcaccgtgaa
     5161 attccgcgaa gacctcgcca atggcgccac gctggacgac ctgctggtgc cggcctttgc
     5221 gacggtgcgt gaagcggccc gccgcgtgct gggcctgcgc cccttcgacg ttcagctgat
     5281 cggcggcatg gttctacacg atggcggcat tgccgaaatg cgcaccggcg aaggcaagac
     5341 gctggtggcg accgcccccg tctacctcaa cgccctggcc cagaagggcg tgcatgtcgt
     5401 taccgtcaac gattatctcg cctcgcgcga cgccgaatgg atgggccgcg tctacaaatt
     5461 cctcggcctg agcatcggca tcatcgtcca tggcctggac gacgaggagc gcagcgccgc
     5521 ctatgcctgc gacgtcacct acgcgaccaa caacgagctt ggcttcgact atctgcgcga
     5581 caacatgaaa tacgagcgct cgcagatggt gcagcgcggt cacaattacg cgatcgtcga
     5641 cgaggtcgac tcgatcctga tcgacgaggc gcgcacgccg ctgatcattt ccggcccgct
     5701 cgaagaccgc tcggaaatgt acaacaccat cgacactttc atgctgaggc tcagccccgc
     5761 cgactatgag atcgacgaga agcagaagac gtcgatcttc accgaggaag gcaccgagaa
     5821 gctcgaaaac atgctgcgcg acgccgacct gctgaaggga gagtcgctct atgatgtcga
     5881 gaacgtcgcc atcgtccacc acgtcaacaa tgcgctgaag gcccaccagc tgttccagcg
     5941 cgacaaggac tatatcgtcc gtaacggcga aatcgtcatc atcgacgagt tcaccggccg
     6001 catgatgccc ggccgccgct attcggaagg cctgcaccag gcgctggaag ccaaggagca
     6061 cgtccagatc cagccggaga accagacgct cgcctccgtc accttccaga actatttccg
     6121 cctctacaag aagcttgccg gcatgaccgg cacggcgctg acggaagccg aggagttcgg
     6181 caacatctac ggtctcgaag tgaccgagat tccgaccaac gttccggtcc agcgcatcga
     6241 cgaggatgac gaggtctacc ggaccgtcga ggaaaaatac aaggccatcg tgcgtgagat
     6301 caaggaagcc cacgccaagg ggcagccgac gctggtgggc accacttcca tcgagaaatc
     6361 cgagcagctg gccgagcgcc tgcgcaagga cggcttcaag gatttcgaag tgctgaacgc
     6421 gcgccaccac gagcgtgagg cctctatcgt cgcgcaggcg ggcaagcccg gctccatcac
     6481 catcgccacc aacatggccg gtcgcggcac cgacatccag ctcggcggca acgccgacat
     6541 gcgcatcgac gaggaactgg ccgacgttcc ggaaggtccc gagcgcgatg cccgcgaaaa
     6601 ggcgatccgc gaagacgtgc agcgcctgaa ggacaaggcg ctcgccgccg gcggccttta
     6661 cgtgctggcc accgagcgcc acgaatcgcg ccgtatcgac aatcagcttc gcggccgctc
     6721 cggccgccag ggcgatcccg gccgttcgaa attcttcctt tcgctgcaag acgatctgat
     6781 gcgcatcttc ggttccgagc gcatggacgg catgttgcag aagctcggcc tcaaggaaga
     6841 cgaggcgatc atccatccct ggatcaacaa ggcgctggaa aaggcgcaga ccaaggtaga
     6901 ggcgcgcaat ttcgacatcc gcaagaacct gctgaaatat gacgacgtct ccaacgacca
     6961 gcgcaaggtg gtgttcgagc agcgcatcga actgatggac ggcgaaggcc tgaccgagat
     7021 catcgccgag atgcgcgagg gcgtcatcga cgagatcgtc gccaaggcca ttcccgaaaa
     7081 tgcctatgcc gagcagtgga acgttgccgg c
//
LOCUS       DASYCS010000119         8542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1475720,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000119 DASYCS010000000
VERSION     DASYCS010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 8542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8542
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1475720"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="VGM46_04200"
     CDS             <1..269
                     /locus_tag="VGM46_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018429955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEY4191825.1"
                     /translation="PHEEAKLLDPQIMLVPLAAFDARGHRIGYGGGYYDRAIERLAKA
                     GKRPRLIGIAFDCQQVEKVPEEPHDAVLAEILTESGLRRFGPTG"
     gene            333..1157
                     /locus_tag="VGM46_04205"
     CDS             333..1157
                     /locus_tag="VGM46_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEY4191826.1"
                     /translation="MRLLFLGDMVGKSGRTAVWEQLPGLISDFRLDFVIVNGENAAGG
                     FGITEEIFRETLAAGADVVTTGNHVWDQRDALAFAPREQRFLRPSNFPKGTPGRGSGV
                     YLAKNGARVLVANIMGRVFMHPELDDPFQAGERELAACPLGEQADAAVIDFHAEATSE
                     KMCFAHFVDGRASLVVGTHTHQPTADHQILNGGTAYISDAGMCGDYDSSLGMDKEEPL
                     NRFLSKVPKGRFEAANGPATICGVGLDISDRTGLAEKIAPFRRGPRLEETAPSFWS"
     gene            1230..1994
                     /locus_tag="VGM46_04210"
     CDS             1230..1994
                     /locus_tag="VGM46_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006327632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEY4191827.1"
                     /translation="MPIIDFLAPHFAFVSDPTAWVALVTLIVLEIVLGIDNLIFISIL
                     TNKLPETQRARARRLGISAALVMRLVLLATISIIVQLTTPVFTAFGHGFSWRDLILIA
                     GGLFLVWKATKEIHHTVDKDDHQDTMLGETLKMSLAGAIFQILLLDLVFSIDSIITAV
                     GMTDEIAIMYIAVIVAVSVMMLAAGPLADFIARNPSIVMLALGFLLMIGMTLIADGMG
                     YHVPKGYIYAAMGFSALVEALNMLARRRRKSGTGGR"
     gene            2141..2890
                     /locus_tag="VGM46_04215"
     CDS             2141..2890
                     /locus_tag="VGM46_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY4191828.1"
                     /translation="MAGHSQFKNIMHRKGRQDAVRSKMFSKLAREITVAAKTGTPDPS
                     MNPRLRLAVQNAKAVSMPKDNIQRAINKASMGDAENYEEVRYEGYGPGGIALIVEALT
                     DNRNRSASNVRAAFTKAGGVLGETGSVSFMWDRVGEIFYPAAAGNAEKVMDAAIEAGA
                     DDVQSDEDGHTIMCAFEGLNDVSKALEGALGEAESVKIIWKPQNNVPVDEERAQSLMK
                     LVATLEDDDDVQNVYANFEVDDETMAKLSAA"
     gene            3057..3347
                     /locus_tag="VGM46_04220"
     CDS             3057..3347
                     /locus_tag="VGM46_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017485377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SelT/SelW/SelH family protein"
                     /protein_id="HEY4191829.1"
                     /translation="MPSPPVIRITYCTQCQWLLRAGWMAQELLSTFGADLGEVALVPG
                     TGGIFTIDCNGEIIWDRKRDGGFPDAAQLKQKVRDVIDPGRDLGHSDRKHKG"
     gene            3562..4806
                     /locus_tag="VGM46_04225"
     CDS             3562..4806
                     /locus_tag="VGM46_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010657547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4191830.1"
                     /translation="MIPSADTPAAQRFAAFRHRPFLSYWAARFISTFATQIVSVAVGW
                     QVYDLTRDPFDLGIVGIVQFLPALLLVLVTGVIADRFGRRLIMAVAIVVEAACAGALL
                     FLTLRGGGGGPLPIFAILAAFGVARAFFGPASSSLFANLVPPRDFANAIAWNSSAWQM
                     ATITGPVAGGLLYGFSPQAAYIAAALLMLVAAGLILTIPKPTQKNETTKATVQTLFAG
                     FGYIWGEKVVLGAISLDLFAVLLSGATALLPVYARDILQLGPWGLGLLRSAPGIGAIT
                     VAIWLAGHPIRNHAGVIMLGFVAMFGAFTIVFGLSTIPWLSIAALVLLGATDMFSVYV
                     RETLIQLWTPDEVRGRVNAVNQVFVGASNELGEFRAGTMAALIGTVPAVVIGGVGAVA
                     VAGIWAYLFPALRNVRHLNSRG"
     gene            complement(4808..5557)
                     /locus_tag="VGM46_04230"
     CDS             complement(4808..5557)
                     /locus_tag="VGM46_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEY4191831.1"
                     /translation="MPSHIARQQSRPKTLPKILPKILIVLHQENSSPGRVGHMLIEAG
                     FDLDIRRPPLGDALPETLENHAGAVMFGGPMSANDSDEFVGRERNWLSVPLKENRPFL
                     GICLGAQMLVNHLGGKVEGHGDGLVEIGWYPLKATEAGEKLMQWPQHVYQFHREGFSL
                     PRDATLLATAETYPNQAFRYGDNAWGIQFHGELTRLMMHRWVVHGAHRFVLPGAQPGR
                     DHLGGRLIWDMQLKRWLAEFLQTVFGAPAAR"
     gene            complement(5613..6095)
                     /locus_tag="VGM46_04235"
     CDS             complement(5613..6095)
                     /locus_tag="VGM46_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006201148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HEY4191832.1"
                     /translation="MFERVLSFFKDLPGGSGDGGIGEDDPRIAASALLYHVMDADGVR
                     QDVEWAKFKAALSQRYGVTGAELDALAKAGERADGEAVDLYAFTSVLKRQLDMDARRV
                     FIGLMWEIVYADGELHELEDNVVWRVAELLGVERRDRIEARQKAAAGSPAAKGISSDE
                     "
     gene            complement(6250..7851)
                     /locus_tag="VGM46_04240"
     CDS             complement(6250..7851)
                     /locus_tag="VGM46_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HEY4191833.1"
                     /translation="MIRILAFLAVVFALGLGFAWLADRPGDMVVTFNGYQYQVSLMVA
                     AVAVVAVVAAVMIVWWLVKSVWNSPYAIARHFRVRRRDRGYQALSTGMIAAGAGDAAL
                     ARRKTREAAKLIRSDQEPLIHLLDAQASLLEGDHEAARGKFEAMLDDPEMRLLGLRGL
                     YLEAERLGDRNAARHYAGRAAQAAPQLAWAAESTLEELARRGDWDGALKLVNAQKSTR
                     QIEPAAANNRRAVLLTAKAQALFDGDPEAARIAAVEANRLRPEFAPAAVIAARALFRN
                     NDVRKGSKVLEAAWKAEPHPEIAELYTHARPGDAVLDRLHRARKLQEMKKNNAESSMA
                     VARAALDAQDFATARREAEAAIRIDPREGAFLLLADIEEADSGDQGKVRQFLSRAVRA
                     PRDPAWVADGVVSERWAPVSPVTGRLDAFEWRAPMERLGQLIDSEEDRPDLSPSLAPP
                     IEAPVVDKADRQAARRETGAAVQRQVADDEPIAPVTAAEFAAVAPDAEAVETSAGTMR
                     LPDDPGIEPEETEDATKSASRFRLF"
     gene            complement(7861..>8542)
                     /locus_tag="VGM46_04245"
     CDS             complement(7861..>8542)
                     /locus_tag="VGM46_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEY4191834.1"
                     /translation="LGPAGGSAPVDLDPLQEKLAALDASVKGAGDNAAQQQSRLGALE
                     QSVSQLTAKVDAQASQPKVALSIAASALKAALDRGAPFTAEFETFAAIAPDAPQLAAL
                     RPYAEKGVASRTDISAGINAAATAMVAAAMPADEDAGFFQNLMSSAETLVTVRPTAAV
                     EGPGVPETVSRMEAAVVQGNYAKALSEYDTLPDAAKAAGADFAARLKARADAEAQVDA
                     LVADAMKG"
BASE COUNT         1452 a         2954 c         2576 g         1560 t
ORIGIN      
        1 gcccgcatga ggaggcgaag ttgctcgacc cgcaaatcat gctggtgccg ctcgccgcct
       61 tcgacgcgcg cggccaccgc atcggctatg gcggcggcta ttacgaccgc gccatcgaac
      121 gcctggcaaa ggccggcaaa cggccgcgcc tgatcggcat cgcctttgat tgccagcagg
      181 tggaaaaggt gccggaagaa ccgcacgacg cggttctggc cgaaatcctg accgaaagcg
      241 gcttgcgccg cttcggccca actggctaga aaggatctgg tttcagaatg cgcggtcttt
      301 tttgagcgcc atgcagtttt cgggggcgcg ccgtgcggct gctttttctc ggtgatatgg
      361 tcggcaaatc cggacgtacg gcggtgtggg aacagcttcc cggcctgatc tcggatttcc
      421 ggctcgattt cgtcatcgtc aatggcgaga acgccgccgg cggtttcggc atcaccgagg
      481 aaatctttcg cgagacgctg gccgccggcg ccgatgtcgt caccaccggc aaccatgtct
      541 gggaccagcg cgacgcgctg gccttcgcgc cgcgcgaaca gcgcttcctg cgcccctcca
      601 atttccccaa gggcacgccg ggccggggtt cgggcgtcta tctggcgaag aacggcgcgc
      661 gcgtgctggt cgccaacatc atgggccgcg tcttcatgca ccccgaactc gacgacccgt
      721 tccaggccgg cgagcgcgag cttgccgcct gcccgcttgg cgaacaggcc gacgccgccg
      781 tgatcgactt ccacgccgag gcgacctcgg aaaagatgtg ctttgcgcat ttcgtcgatg
      841 gccgcgcctc gctggtcgtc ggcacgcata cccaccagcc gactgccgac caccagatcc
      901 tcaatggcgg caccgcctat atttccgacg ccggcatgtg cggcgactat gattcatcgc
      961 tgggcatgga caaggaagaa ccgctgaacc gcttcctgtc gaaagtgccg aaaggccgct
     1021 tcgaggcggc caatggcccc gccaccatct gcggcgtcgg cctcgacatt tccgaccgca
     1081 ccgggcttgc cgaaaagatc gcccctttca ggcgcggacc ccggcttgaa gaaaccgcgc
     1141 cttccttctg gtcttgacat tgatccagtg cttcgccgta cctaacttcg ggcgcaacaa
     1201 gccgtagaca tgacgagggg atcgccgcca tgccgattat cgacttcctg gcaccacact
     1261 tcgcctttgt cagcgacccg acagcctggg tggcgctggt aacgctgatc gtgcttgaga
     1321 tcgttctcgg catcgacaat ctgatcttca tctcgatcct caccaacaag ctgccggaaa
     1381 cgcagcgcgc ccgcgcccgc cggctgggca tttcggcggc gctggtcatg cggctggtgc
     1441 tgctcgccac catttccatc atcgtgcagt tgaccacgcc ggtcttcacc gccttcggcc
     1501 acggcttttc ctggcgcgac ctgatcctga tcgccggcgg cctgttcctg gtctggaagg
     1561 cgaccaagga aatccaccac accgtcgaca aggacgacca tcaggacacc atgctgggcg
     1621 agacgctgaa aatgtcgctg gccggcgcga tcttccagat cctgctgctc gacctggtgt
     1681 tttccatcga cagcatcatc accgccgtcg gcatgaccga cgagatcgcc atcatgtaca
     1741 tcgccgtcat cgtcgccgtt tctgtgatga tgctggccgc cggcccactc gccgacttca
     1801 tcgccaggaa ccccagcatc gtcatgctgg cgctcggttt cctgctgatg atcggcatga
     1861 cgctgattgc cgacggcatg ggttaccatg tgcccaaggg ctacatctac gccgccatgg
     1921 gcttctccgc gctggtcgag gcgctgaaca tgctggcgag gcggcgcagg aagtcgggaa
     1981 cgggcgggcg ctagccttcg tctcatggcg ccagtggcca tgtctggacg aattgagccg
     2041 atttatctat aagcccgcca ttatcgaaaa atggcgggtt gccgtttgcg gccatgcgga
     2101 cgccgcgcga cccgaccgcc gctaagacga cagggaaccc atggccggcc attcacagtt
     2161 caagaacatc atgcatcgca agggccgcca ggacgcggtg cgctcgaaga tgttttccaa
     2221 gctcgcccgc gaaatcaccg tcgccgccaa gaccggcaca cccgatccgt cgatgaaccc
     2281 gcgcctgcgc ctggctgtgc agaacgccaa ggcggtgtcg atgcccaagg acaacatcca
     2341 gcgcgccatc aacaaggctt cgatgggcga cgccgaaaac tatgaggaag tgcgctatga
     2401 aggctatggc cccggcggca tcgccctcat cgtcgaggcg ctgaccgaca accgcaaccg
     2461 ttccgcctcc aacgtccgcg ccgctttcac caaggccggc ggcgtgctcg gcgaaaccgg
     2521 ctcggtttcc ttcatgtggg accgcgtcgg cgagattttc tatcccgccg ccgccggcaa
     2581 tgccgaaaaa gtcatggatg cggcaatcga ggccggcgcc gacgacgtgc agtccgacga
     2641 agacggccac accataatgt gcgccttcga aggcctgaat gacgtatcca aggcgctgga
     2701 aggcgcgctt ggcgaggcgg aatcggtgaa gatcatctgg aagccgcaaa acaacgtgcc
     2761 cgtagacgag gaacgcgcgc agtcgctgat gaagctggtc gccacgctgg aagacgatga
     2821 cgacgtgcag aacgtctacg ccaatttcga ggtcgacgac gagacgatgg ccaagctcag
     2881 cgcggcgtga accgcgtcgg tcttgacctc ggcacgaccc gcaaatggca gcttttcggc
     2941 actctcggtg cttaattgcc gggatataca ctccgttccg gctctcgacc ccccattccg
     3001 gctcgacttg agccggaatt cggcacagtt ctctccgtag aaagattgat cgcgccatgc
     3061 cctcgccgcc cgtcatccgc atcacctact gcacgcagtg ccaatggctg ttgcgggcag
     3121 gctggatggc gcaggagctt ctgtcgacct tcggcgccga ccttggcgaa gtggcgctgg
     3181 tgcccggcac cggtggcatc ttcaccatcg actgcaacgg cgaaatcatc tgggaccgca
     3241 agcgcgacgg cggctttccc gacgccgcgc aactcaagca gaaggtgcgc gacgtcatcg
     3301 accccggtcg cgacctcggc cactccgacc gcaagcacaa aggctgaccg gcttttcgca
     3361 aaaggccgac caagccagcc gcgccgaacc cgcccaccgg gcgcaagccg gtaaaaaccg
     3421 cgccggcatc tttttcttcg aaggcacggc gtaaaaaaaa gcgggcgtcg gatatggaat
     3481 tgcgataatg gcatccggcc ggatcagcgc ccattctgga agtgccccgc cggccaagca
     3541 ccgcaatcgg ggccaccgaa cgtgatacct tccgccgata cgccagccgc ccagcgtttt
     3601 gccgccttcc ggcaccggcc gttcctgagt tactgggccg cgcgcttcat cagcaccttc
     3661 gccacgcaga tcgtgtccgt cgccgtcggc tggcaggtct acgacctgac ccgcgatccg
     3721 ttcgacctgg gcatcgtcgg catcgtccag ttcctgcccg cgctgcttct ggtcctggtt
     3781 accggcgtca ttgccgaccg cttcggccgc cgccttatca tggcggtggc gattgtcgtg
     3841 gaggcggcgt gcgccggcgc gcttttgttt ctcacccttc gcggcggtgg tggcggccct
     3901 ttgccgatct tcgccatact ggccgccttc ggcgttgccc gcgccttttt cggcccggct
     3961 tcgtcgtcgc tgttcgccaa tctggtgccg ccgcgggatt tcgccaacgc cattgcctgg
     4021 aactcctccg cctggcagat ggcgaccatc accgggccgg tggccggcgg cttgctatac
     4081 gggttttcgc cgcaggccgc ctatatcgcg gccgccctgc tgatgctggt cgccgccggc
     4141 ctgatcctca ccattccaaa gcccacgcag aaaaacgaaa caaccaaggc gaccgtgcag
     4201 acgctgttcg ccggcttcgg ttatatctgg ggcgagaagg tcgtgctcgg cgccatctcg
     4261 ctcgacctgt tcgccgtgct gctttccggc gcgaccgcgc tgctgccggt ctatgcgcgc
     4321 gatattctcc agcttggacc atggggcctc ggcctgctgc gctcggcccc cggcataggc
     4381 gccatcaccg tcgccatctg gcttgccggc catccgatcc gcaaccatgc cggcgtcatc
     4441 atgctcggtt tcgtcgccat gttcggcgcc ttcaccatcg tgttcggcct gtcgacgata
     4501 ccctggctgt ccatcgccgc gctcgtcctg cttggcgcga ccgacatgtt cagcgtctat
     4561 gtgcgcgaaa cgctgatcca gctatggacg ccggacgagg tccgtggccg cgtcaacgcc
     4621 gtcaaccagg ttttcgtcgg cgcttccaat gaactgggcg aattccgcgc cggaaccatg
     4681 gccgccttga tcggcacggt gccggccgtg gtgatcggcg gcgtcggcgc ggtcgccgtc
     4741 gccggcatct gggcttacct ttttccggcg ttgaggaacg tgcgccacct caacagccgc
     4801 ggctgagcta tcgcgccgcc ggcgcgccga acacggtttg caggaactcg gccagccagc
     4861 gcttcagctg catgtcccag atcagccggc cgccgagatg atcgcggccg ggctgcgcgc
     4921 cgggaagcac gaagcgatgc gccccgtgca ccacccagcg gtgcatcatc agccgggtca
     4981 gttcgccgtg gaactggatg ccccatgcat tgtcgccata gcgaaacgcc tggttgggat
     5041 aggtttccgc cgtcgccagc agcgtcgcgt cgcgcggcaa cgaaaagccc tcgcggtgga
     5101 actggtagac atgctgcggc cattgcatca gcttttcgcc ggcctccgtc gccttgagcg
     5161 gataccagcc gatttccacc agcccgtcgc catggccttc gaccttgccg cccagatgat
     5221 tgaccagcat ttgcgcgccg aggcagatgc cgagaaaagg ccggttttcc ttcagcggga
     5281 cagacagcca gttgcgctcg cggccgacga actcgtcgct gtcattggcg ctcatcggcc
     5341 cgccgaacat caccgcgccg gcatggtttt ccaacgtttc aggcagcgcg tcccccagcg
     5401 gcggacggcg gatgtcgaga tcgaaccccg cctcgatcag catatggccg acccgtcccg
     5461 gactggaatt ttcctgatgg agcacaatca ggatttttgg caggattttt ggcagggttt
     5521 ttggccggct ttgctgccgg gcgatgtgtg atggcatttt cttgaaaagt cccgaaattc
     5581 gtctgcccca gcccgttcgt cgctgatatt gcctattcgt cgctggatat tccctttgcg
     5641 gcgggcgatc cggcggcggc cttctggcgt gcttcgatac ggtcccggcg ctcgacgcca
     5701 agcagttcgg caacccgcca taccacattg tcttccagct cgtgcagttc gccgtcggca
     5761 tagacgatct cccacatcag gccgataaag acccggcggg catccatgtc gagctgccgc
     5821 ttcaacacgc tggtgaaagc gtacagatcg accgcttcgc cgtccgcccg ttcgccggcc
     5881 ttggccagcg catccagttc cgcaccggtc acaccatagc gttgcgaaag cgcggccttg
     5941 aatttcgccc actccacgtc ctggcgcacg ccatcggcat ccatgacatg atagagcagg
     6001 gcggaggcgg cgatgcgcgg atcgtcctcg ccgataccgc catctccgct gccgcccggc
     6061 aaatccttga agaaggacaa tacccgctcg aacatcaggc tactccaatt cgtcgcatcg
     6121 atttagagca tttccagcaa aagtattgaa acggttttgc atccggaaat acgcccaaaa
     6181 caaagagata gagcggtttt accgattcgg taaaaaccag aaccgctcta tcgcgccagc
     6241 ggatcacgat caaaacaaac gaaagcgact ggccgatttt gtcgcgtcct ccgtctcttc
     6301 cggctcgatg ccgggatcgt ccggcaggcg catcgtcccg gcggaagttt cgaccgcttc
     6361 cgcgtcgggc gcaacggcgg cgaattcggc ggcggtgacc ggcgcgatcg gctcgtcatc
     6421 agccacctgc ctttgaaccg ccgcacccgt ttcccgcctt gcggcttggc gatcggcctt
     6481 atcgacgacc ggcgcctcga tcggcggggc cagcgatggc gacaaatccg gcctgtcttc
     6541 ctcgctgtcg atcagctggc cgagccgctc catcggcgcg cgccactcga aggcgtcgag
     6601 cctgccggtg accggcgaaa ccggcgccca gcgctccgaa acgacgccat cggcaaccca
     6661 tgccgggtcg cgcggcgcgc gcaccgcccg cgacagaaac tgccgcacct tgccctggtc
     6721 gccactgtcg gcctcctcga tgtcggccag cagcaggaag gctccctcgc gggggtcgat
     6781 gcgaatggcg gcttcggctt cgcgccgcgc cgttgcaaag tcctgcgcat cgagcgcggc
     6841 acgcgccacc gccatcgaag attcggcgtt gttcttcttc atttcctgca atttccgcgc
     6901 gcggtgcaga cggtcgagca cggcgtcgcc cggccgggca tgcgtataaa gctcggcgat
     6961 ttcgggatgc ggctcggcct tccatgccgc ctccagcact ttcgagcctt tccgcacgtc
     7021 attattccga aacagcgccc gagccgcgat gacggctgcc ggcgcgaatt ccgggcgcag
     7081 gcggttggcc tcgacggccg ctatccgcgc cgcttcggga tcgccatcga acagggcctg
     7141 cgccttggcc gtcagcagca cggcgcgccg attattggcg gccgcaggct cgatctggcg
     7201 tgtcgacttc tgcgcgttga ccagcttgag cgcgccgtcc caatcgccgc gccgggccag
     7261 ttcctccagc gtcgattcgg ccgcccacgc cagttgcggc gcggcttgcg ccgcccggcc
     7321 ggcatagtgg cgggcggcgt tgcggtcgcc cagccgctcg gcctccagat aaagcccgcg
     7381 caggccgagc aaccgcattt cgggatcgtc gagcatcgcc tcgaacttgc cgcgggccgc
     7441 ctcatggtcg ccctccagca gcgaagcctg cgcgtcgagc agatggatca gcggctcctg
     7501 gtcggagcgg atcagcttcg ccgcttccct tgttttcctg cgcgccagcg ccgcgtctcc
     7561 tgccccggcg gcaatcatgc ccgtcgatag cgcctgatag ccacggtcgc ggcggcgcac
     7621 gcggaaatgc cgcgcaatcg cataggggct gttccagacc gatttaacca gccaccagac
     7681 aatcatcacg gcggcaacga ccgccacgac cgccaccgcc gccaccatca ggctgacctg
     7741 gtactgatag ccgttgaagg tcaccaccat gtcgcccggc cggtcggcca gccaggcaaa
     7801 gccgaggccg agcgcgaaga cgactgcaag gaaggcgagg attcggatca tgaagcgtcc
     7861 tcaccccttc atggcgtcgg cgaccagcgc atcgacctgc gcctcggcgt ctgcccgcgc
     7921 cttgagcctg gcggcgaaat ccgcgccggc ggccttcgcc gcatccggca gcgtgtcata
     7981 ttcggacagc gccttggcat agttgccctg cacgacagcc gcctccatgc gcgacaccgt
     8041 ttcgggcacg cccggacctt ccaccgcagc cgtgggccgc accgtgacca gcgtttcggc
     8101 gctcgacatc aaattctgga aaaagccggc atcctcgtcg gcaggcattg ccgcagccac
     8161 cattgcggtc gccgccgcat tgatgccggc tgaaatgtcg gtgcggctgg ccacgccctt
     8221 ttccgcatag ggacgcaacg ccgcaagctg cggcgcgtcc ggcgcgattg ccgcgaaggt
     8281 ttcgaactcc gctgtaaacg gcgcgccgcg atccagcgcc gccttcagcg ccgaagccgc
     8341 gatagacagc gccacctttg gctgcgacgc ctgcgcgtca accttggccg tcagctgcga
     8401 caccgattgc tcaagcgcgc cgagccggct ttgctgttgg gcggcgttat cgccggcgcc
     8461 cttcaccgac gcgtcgagcg ccgccagttt ttcctgaagc ggatcgagat cgaccggtgc
     8521 cgaaccgcct gccgggccaa gc
//
LOCUS       DASYCS010000120         9753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3215576,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000120 DASYCS010000000
VERSION     DASYCS010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 9753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9753
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3215576"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="VGM46_04250"
     CDS             complement(<1..693)
                     /locus_tag="VGM46_04250"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEY4191835.1"
                     /translation="MAVAFTFPGQGSQAVGMGKELADAFPESRRVFDEVDEALGEKLS
                     DLIWDGPESSLTLTANAQPALMAVSLAAVRALEARGFSLKDKVAYAAGHSLGEYSALA
                     ASGFVSVADAARLLRIRGNAMQAAVPAGEGAMAAIIGLEQADVEAACGEAAKGSVCQI
                     ANDNGGGQLVISGAKQAVELAAKLCTEKGAKRALMLQVSAPFHSALMGPAAEAMREAL
                     ATVQKHAPVVPVV"
     gene            934..2862
                     /locus_tag="VGM46_04255"
     CDS             934..2862
                     /locus_tag="VGM46_04255"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEY4191836.1"
                     /translation="MAKNIAGMAVKAPDSSLDKIGDRAGRARKFWRASAYSLVSLLAA
                     TTLVFAIELIFRGNLPATVEFFLQPFKPGWTTIVVFTLILIGLDALLGRKHQSLMIVA
                     PLTLVLAFIGHQKSLYLGDPLYPTDFLYARQIVELMPLLVRERPLTGLAMAVGLVVFL
                     AILVFAWRLWRRRMPVLTYRGRIARLVLTLPLLAFFVSIMDYATFSWTRDRLQIIPIM
                     WDQKENYNSNGFALAFALNVPMAHVTAPAGYSDKAVAAIARPDTAAAVPAEKPDIIVV
                     MSESFWDPTKLPNVAITPDPIPTVRELRSGSMFSPEFGGMTANIEFEALTGFSNAFLP
                     AGSIPYQQYVRAPIPSVATFLKSEGYATRAIHPGTSWFWNRTPVYADFGFDDFKSEEN
                     LPPLQKRGPLASDAAMTDEIIREADETSDPFFFFAVSLQNHGPYEPHRYSDPTHSVQA
                     QTSQWAKDSLLSYTEGAADADRGLQRLLDWAKQRKRPTVIAFFGDHLPPLGPVYVETG
                     FLKDNVAPRKEAPEQMLEHHQTPLVVWSNKTGPVKDMGAVSPAFLPYHILTTAGITHP
                     YYTGFLGELNRRYHVVDRNLLLSAQGDATPDWARQKEIDPAIRDFRFLQYDMMFGKRR
                     AAADFFPETVNKLVAHTS"
     gene            3033..3200
                     /gene="rpmG"
                     /locus_tag="VGM46_04260"
     CDS             3033..3200
                     /gene="rpmG"
                     /locus_tag="VGM46_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011179398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEY4191837.1"
                     /translation="MAKAANIKIKLLSSADTGFFYVTSKNSRTKTDKLSFRKYDPIAK
                     KHVEFKETKIK"
     gene            complement(3622..4995)
                     /locus_tag="VGM46_04265"
     CDS             complement(3622..4995)
                     /locus_tag="VGM46_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019858398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PleD family two-component system response
                     regulator"
                     /protein_id="HEY4191838.1"
                     /translation="MTARILVVDDVPANVKLLEVRLLAEYFEVLTATNGPDAIETCEN
                     GKVDVVLLDVMMPDMDGFEVCKRLKRDPATSHIPVVMITALDQVSDRVRGLEAGADDF
                     LTKPVNDLQLMTRVKSLVRLKMLTDELRLRASTTRNIGIEELLSRTFNSADTKPKVLL
                     VDERKSSFERVQKVLRDSAELDISTDPHAGFFQAAETPYDCVIVSTAFSDFDPLRLCS
                     QLRSLDRTRFVPIILLAEAGEDDRIIRALELGVNDYLVRPIDPHELTARLRTQVRRKR
                     YNDQLRASVTQTIEMAVTDGLTGLHNRRYLDSHLQTLFDRAVTRRRPLSMMITDLDRF
                     KSINDTYGHDGGDEVLREFARRLRKNVRGIDLACRFGGEEFVVVMPDTDGFVAEKVAE
                     RIRAEIAQQPFAIGRDGKTIEVTVSVGVSSVLKGQDTVEALMKRADLALYEAKSGGRN
                     RVVAKAA"
     gene            complement(5032..5388)
                     /locus_tag="VGM46_04270"
     CDS             complement(5032..5388)
                     /locus_tag="VGM46_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY4191839.1"
                     /translation="MIVEDNELNMKLFRDLIEASGYETVRTRNGLEALDLARLHKPDL
                     ILMDIQLPEVSGLEVTKWLKEDDELHVIPVIAVTAFAMKGDEERIRQGGCEAYISKPI
                     SVPRFIETIKSYLGDA"
     gene            5651..5950
                     /locus_tag="VGM46_04275"
     CDS             5651..5950
                     /locus_tag="VGM46_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006334977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3572 domain-containing protein"
                     /protein_id="HEY4191840.1"
                     /translation="MASAASMQEEAEAVAVSALGFVAADPELLPRFLAITGIEASSIR
                     QAAREPGFLAGVLQFILAHEPTLLRFAEETGMPPASIGRALRALPTGADDQEYFA"
     gene            5947..6609
                     /locus_tag="VGM46_04280"
     CDS             5947..6609
                     /locus_tag="VGM46_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191841.1"
                     /translation="MSDDPETARQIEELAADDRPLLVLDVDDVLLEFIRPFPRFLETR
                     GLGLTLNSFRLTGNIADKASGRLAEQTEVTALLDEFFQGQADWQSITDGATEALATLG
                     KHAEIVLLTAMFHRHRAVRRAHLDALGLTYPLLTTEMAKGPAVAKLRGATARPVAFVD
                     DQPHNLVSVRQAVGDAHLFHLMADNSLRQFLPPVTDDITTVQDWREAAPKIAKALGLA
                     AG"
     gene            6699..8051
                     /locus_tag="VGM46_04285"
     CDS             6699..8051
                     /locus_tag="VGM46_04285"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEY4191842.1"
                     /translation="MAIPLNKPDHGFCRDCLTLQHGPVGRCERCGSPRLARHGELYGL
                     HIAHIDCDAFYAAVEKRDNPALRDRPLIIGGGKRGVVSTACYIARINGVRSAMPMFKA
                     LEACPEAVVIKPDMEKYVRVGREIRAMMQALTPLVEPISIDEAFLDLGGTEKLHGLPP
                     ALVLARFVQAVEKEIGITVSAGLSYCKFLAKIASDFRKPRGFSVIGQAEAVGFLADKP
                     VTMIWGVGKAFAATLEQDGVRTIGQLQRMERGDLLRRYGTMGDRLYRLSRGEDERRVV
                     SDRDSKSVSAETTFDTDLASADDLVPVLRALSEKVSARLKKAGLSGRTVVLKLKTQDF
                     KTRTRNRQLEDPTRLADRIFQTGLHLIGKELDGTRYRLLGIGVSDLADSQKADPPDLV
                     DVQARKRAMAEGAIDALRDKFGGKAVETGYTFGKGHRGRPARPDEDEGPEVQHPWERI
                     "
     gene            8239..9120
                     /locus_tag="VGM46_04290"
     CDS             8239..9120
                     /locus_tag="VGM46_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008122289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY4191843.1"
                     /translation="MTPAPSLIKAALWMALSIASFLGMTVASRETTRVLNVFEVLELR
                     SVIGLLFLMPLVVRSGGFAAMRTQRPWMHIGRNCAHYAGQGAWLYALTLIPLAVLVSI
                     EFTTPIWTAVLAVSFLGEKLSKARVASIVLGLIGVTIIVRPGVGAVSLGELVVLAAAV
                     SFGVSMVMVKSMTRTESVVRIIFWMLVIQSALGFIPALVHWQNPPLELWPWVLLIGFT
                     GMSSHFFLARALTYADATLISPMDFLRVPGTALVGWALYGEQIDVYTATGATLILMGN
                     LLNLQKRRRAPIGAAQT"
     gene            complement(9117..>9753)
                     /locus_tag="VGM46_04295"
     CDS             complement(9117..>9753)
                     /locus_tag="VGM46_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006334992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HEY4191844.1"
                     /translation="LQETRSFLWVDYLVAAIVAADMLARLLASTDMLRLMRQPTWLVD
                     FFILLTLLFPTALANFGFLRILRLWSLSRSGVLWRHLEMRRLRDWREASHAVLNLLTF
                     LLVVTGFVYTFFFRDGSGLAGYVDALYFTVATVTTTGFGDIVLPGMAGKLTAIVTMII
                     GISLFVRLAQSVFRPSKVFFPCPQCGLQRHEPDAVHCKACGHLLNIPDNND"
BASE COUNT         1894 a         3138 c         2835 g         1886 t
ORIGIN      
        1 aaccaccggc accaccggcg catgcttttg caccgtggcg agcgcctcgc gcatggcctc
       61 ggccgccgga cccatcagcg ccgaatggaa aggcgccgaa acctgaagca tcagcgcccg
      121 cttggcgccc ttttccgtgc acagcttggc cgccagttcc acggcctgct tggcgccgga
      181 aatcaccagc tggccgccgc cattgtcgtt ggcgatctgg caaacggacc ccttggccgc
      241 ctctccgcat gcagcttcaa catctgcctg ctccagcccg atgatcgcag ccatcgcccc
      301 ttcgccggcc ggcaccgccg cctgcatggc gttgccgcga atgcgaagca gccgggccgc
      361 atccgccacc gaaacgaaac cgctggcggc cagcgccgaa tattcgccca gcgagtggcc
      421 ggccgcgtag gcgaccttgt ccttcaacga aaaaccgcgc gcttccagcg cccgcaccgc
      481 cgccagcgaa accgccatca acgccggctg cgcgttggcg gtcagcgtca ggctggattc
      541 aggcccatcc cagatcaggt cggacagctt ttctccaagc gcctcgtcga cctcgtcgaa
      601 gacgcgccgg gattccggaa aggcgtccgc caattccttg cccatgccga cggcctggct
      661 gccctgcccc ggaaaagtga atgcgacggc catacaggat gctcccgttg tcttgtttcc
      721 gactgctgtt gctcaagcgg cgagttcaag tcaagccgtt taagcttgcc cgccaaagcc
      781 aaactcccgg tttaagaagc ttttttacga tcactcatga ttaaaatcgc acacgatttg
      841 tttgccggct cttcccattt ccgccacaga cgatagttct tcgtgacagt ggtatgacgt
      901 ttggatgacg tctttaagtg gccggggatt agagtggcga agaatattgc aggcatggcc
      961 gtgaaggcgc cggacagttc cctggacaaa atcggggacc gcgctggccg tgcgcgcaaa
     1021 ttttggcgcg cctcagccta tagtctcgtt tcgctgctgg cggcgaccac gctggtattt
     1081 gccatagagc tcatctttcg cggcaatctg ccggcgaccg tcgagttctt cctacagccg
     1141 ttcaagccgg gctggaccac catcgtcgtg ttcacgctca tcctgatagg cctcgatgcg
     1201 ctgcttggcc gcaagcacca gagcctgatg atcgtcgccc cgctgacgct ggttctggct
     1261 ttcatcgggc atcagaaatc gctttatctc ggcgatccgc tttatccgac cgactttctc
     1321 tatgcccgcc agatcgtcga actgatgccg ctattggtgc gcgagcgccc cttgaccggc
     1381 ttggccatgg cggtcggcct ggtcgttttc ctggcaattc tggttttcgc ctggcgcctg
     1441 tggcgtcgcc gcatgccggt gctcacctac cggggccgta tcgcgcggct ggttttgacc
     1501 ctgccgcttc tcgccttctt cgtctcgatc atggattacg cgaccttctc gtggacgcgc
     1561 gaccgcttgc agatcatccc gattatgtgg gaccagaagg agaactataa ttccaacggc
     1621 ttcgcccttg cctttgcgct gaacgtgccg atggcccatg tcaccgcgcc ggccggctat
     1681 tccgacaagg ccgtagccgc gatcgccagg cccgacaccg ccgccgccgt gccggccgaa
     1741 aaacccgaca tcatcgtcgt catgagcgaa tccttctggg atccgaccaa gcttcccaac
     1801 gtcgccatca cccccgatcc cattcccacc gtgcgcgaat tgcggtccgg ctcgatgttc
     1861 tcgccggaat tcggcggcat gaccgccaat atcgagtttg aggcgctgac cggtttttcc
     1921 aacgccttcc tgccggcggg cagcattccc tatcagcaat atgtacgcgc gccgatcccc
     1981 tctgtggcga ccttcctgaa aagcgaaggc tatgccaccc gcgccatcca ccccggcacg
     2041 agctggttct ggaatcgcac cccggtctat gccgatttcg gctttgacga tttcaagtcc
     2101 gaggaaaacc tgccgccgct gcaaaagcgc ggaccgctgg cttccgatgc cgccatgacc
     2161 gacgagatca tccgcgaagc ggacgaaacc agcgatccct tctttttctt tgcggtaagc
     2221 ctgcaaaacc atggccctta cgagccgcat cgctattccg atccgaccca ttcggtgcag
     2281 gcgcagacca gccagtgggc gaaggattcg ctgttgagct atacggaagg ggctgccgac
     2341 gccgaccgtg gattgcagcg cctgctcgac tgggccaagc agcgcaagag gccgacggtg
     2401 attgccttct tcggcgatca cttgccgccg ctcgggccgg tctatgtcga aaccggcttc
     2461 ctgaaggaca atgtcgcgcc acgcaaggaa gcgccggagc agatgctgga gcaccaccag
     2521 acgccactgg tggtctggtc gaacaagaca ggcccggtca aggacatggg cgcggtcagc
     2581 ccggcctttc tgccctatca catcctgacg accgccggca tcacgcatcc ctattacacc
     2641 gggttcctgg gcgaactgaa ccggcgctac catgtcgtcg accgcaacct gctcctgtcg
     2701 gcgcagggcg atgcgacgcc cgactgggcg cgccagaagg aaatcgaccc ggccatccgc
     2761 gatttccgtt tcctgcaata tgacatgatg ttcggcaaac ggcgggcggc ggccgacttc
     2821 ttccccgaaa cggtcaacaa gctcgtcgcc catacgagct gaccggcctt gcggggatcg
     2881 aacgcaagtg tttggtcttc gccggcttcg cattccgcgc acggacttga ctttccgggc
     2941 atgtccttta tgtgtcgcgc caagtttccc gcgcccggat atttcccgtg ctcaagcggc
     3001 gcgaacctca cgacaactga cggacgaaca tcatggccaa agccgcaaat atcaagatca
     3061 agcttctgtc gagcgccgac accggcttct tctacgtgac cagcaagaac agccgcacca
     3121 agaccgacaa gctgtcgttt cgcaaatacg atccgatcgc caagaagcat gtcgaattca
     3181 aagagaccaa gatcaagtaa tctggtttct tcctccagac aaaaaaagcc gcccattggg
     3241 cggctttttt gtttcggcct ctgcgccaag ccgttgaaag ccttgcgaat taggggccgg
     3301 ccgacggttg gcagcggtac gaggaggcca ctatgtgcca gcgccggccg gccgacccca
     3361 cggacccagg agatgcggcg gacctgagca tcatggggta tgctttaaac ggtcccggca
     3421 acttccatgc gccgtacccg tctggcaggt acatttgcgc aagggcttat ataaatcaat
     3481 aatttcttaa ccactggcgc cgaatcatcg aaattaaggt aaactcgtaa ccctgctgtc
     3541 ttgacaggca aaaacgcgga atcgcggatg cacaggccgc atttccggac gcaaaaccgt
     3601 tcacactttt gctggaatgc tctaggcggc cttggcgacc acccggttgc gcccaccgct
     3661 tttggcttcg tagagcgcaa ggtcggcgcg cttcatcagg gcttcgacag tgtcctgccc
     3721 tttcagcacc gaagaaacgc cgacgctgac cgtgacctcg atcgtcttgc cgtcgcggcc
     3781 gatggcgaag ggctgctgcg cgatctctgc acgaatgcgc tcggcgacct tttcggcgac
     3841 gaagccgtcc gtgtccggca tcaccacgac gaattcctcg ccgccgaacc ggcaggccag
     3901 gtcgatgcca cgcacattct tgcgcaagcg ccgcgcgaat tcgcgcaata cctcgtcgcc
     3961 gccatcatga ccgtaagtgt cgttgatcga cttgaagcgg tccagatcgg taatcatcat
     4021 cgacaatggc cgccgccgcg taactgcccg gtcgaacagg gtctgaaggt ggctgtcaag
     4081 atagcggcgg ttgtgcaggc cggtcagccc gtccgtcacc gccatctcga tggtctgcgt
     4141 gacgctggcg cgcagttggt cgttgtagcg cttgcggcgg acctgcgtgc gcaggcgcgc
     4201 cgtcagttca tgcggatcga tcggccgcac gagataatcg ttcacgccaa gttcgagcgc
     4261 gcgaatgatg cggtcatcct cgcccgcctc ggccagaagg atgatcggca cgaaccgcgt
     4321 gcggtcgagc gagcgcagtt gcgagcacag ccgcaaagga tcgaagtcgg aaaacgccgt
     4381 cgacacgatc acgcaatcat aaggcgtttc ggccgcttgg aaaaaaccag catgcggatc
     4441 ggtggagata tcgagttccg cactgtcgcg aagcactttt tggacacgct cgaacgacga
     4501 tttccgctcg tcgaccaaaa gcactttcgg cttcgtgtcg gccgaattga acgtgcggct
     4561 cagcaattcc tcgatcccga tattgcgggt ggtcgaagcg cgcaggcgca gttcgtcggt
     4621 cagcatcttt agccgaacca ggcttttcac gcgcgtcatc aactgaagat cgttgaccgg
     4681 cttggtcagg aagtcgtcgg cgccggcctc aaggccccgc acacggtccg aaacctgatc
     4741 cagagcggtg atcatcacca cgggaatgtg cgatgtcgca ggatcccgct tcagccgctt
     4801 gcagacttca aagccgtcca tgtccggcat catcacgtcg agcagcacga catcgacctt
     4861 gccgttctcg caggtctcga tggcgtccgg gccattggtt gcggtcagca cctcgaaata
     4921 ttcggccaga agccgcactt caagcagctt cacgttggcc ggaacgtcat cgacgacaag
     4981 aatgcgggct gtcatttcaa gaagctccgg agcgacggca ttgggctggc atcaggcatc
     5041 gcccaggtag gatttgatgg tttcgatgaa acgcggcacc gagatcggct tcgagatata
     5101 ggcctcgcag ccaccttgcc gaatgcgctc ctcatcgccc ttcatggcga atgcggtgac
     5161 ggcaatgacc ggaatgacgt gcagttcgtc atcctctttc agccatttcg tcacctccag
     5221 cccggatact tcgggcaact ggatgtccat caaaatgaga tcgggcttgt gcagccgcgc
     5281 caggtcgagc gcttccagcc cgttgcgggt gcgcacggtt tcatagccac tggcctcgat
     5341 gaggtcgcga aagagcttca tgttcagctc attatcttcg acgatcatga ccttcttcgg
     5401 cattttttcc cgtcccggct gccatccgca tggaccgcca ccgcaatgca ttcgcccagg
     5461 aacgcaccaa acccgattgt tactctacgc caagaacatt gacgaaacgc aaacccgcgc
     5521 ccctaaaagc catcgacaat ctgccgctaa cgttcccgta gagacaacgg cgcgaaagca
     5581 gcgtcttgcc gcttgccata cttggcttta cttgaagctg aattcaacgg ttacgtattg
     5641 ggtcgattcg atggcgagtg ctgcgtcaat gcaagaagaa gccgaagccg tcgccgtcag
     5701 cgcgcttggc tttgtcgccg ccgatccgga acttctgccg cgcttcctcg cgatcaccgg
     5761 catcgaggcc tcatcgataa ggcaagcggc gcgcgaaccg ggctttctgg ccggcgtgct
     5821 gcaattcatc ctggcgcatg aaccgacatt gctgcgcttt gccgaggaaa ccggcatgcc
     5881 gcccgcgtcg atcggccggg cattgcgggc cttgccgaca ggcgcggacg atcaagagta
     5941 tttcgcatga gcgacgatcc ggagacggcc cgccagatag aggaactggc ggcggacgac
     6001 cgcccgctgc tggtactcga tgtcgatgac gtgctgctgg agttcatccg ccccttcccg
     6061 cgctttctgg aaacgcgcgg cctcggcctg acgctcaact catttcgcct gaccggcaac
     6121 atcgccgaca aagccagcgg caggctggcc gaacagaccg aggtgaccgc cctgctcgat
     6181 gaattcttcc agggccaggc cgattggcaa agcatcaccg acggcgctac cgaagcgctt
     6241 gccacgcttg gcaaacatgc cgagatcgtg ttgctgacgg cgatgttcca ccgccatcgc
     6301 gcggtgcgcc gcgctcatct cgatgcgctc ggcctgacct atccgctgtt gaccaccgaa
     6361 atggccaagg gtccggcggt ggcaaagctg cgcggcgcaa cggcaaggcc ggtggccttt
     6421 gtcgacgacc agccgcataa tctggtctcg gtccgccagg ccgtgggcga cgcccacctg
     6481 tttcacctga tggccgacaa ttcgctgcgg cagtttctgc cgccggtcac cgacgatatc
     6541 accaccgttc aggactggcg cgaggcggcg ccgaagatcg caaaggcgct cggcctggcg
     6601 gcgggctgaa gcatttctcc cgaccacggc atggcccacc gccttgccgc gatcgcggcg
     6661 ctggcgtacg atcccacctg tttcctcatg gctgcggcat ggcgatccct ctcaacaagc
     6721 ccgatcacgg tttttgtcgc gattgcctga cccttcagca cggtcccgtt ggccgttgcg
     6781 aacggtgcgg cagccctcgg cttgcccgtc atggcgaact atatggcctg cacatcgccc
     6841 atatcgactg cgacgccttc tacgcggcgg tggaaaaacg cgacaatccc gcgctaaggg
     6901 acaggcccct catcatcggc ggcggtaagc gcggcgtggt ttcaaccgcc tgttacattg
     6961 cccgcatcaa cggcgtgcgt tcggccatgc cgatgttcaa ggcgcttgag gcctgcccgg
     7021 aggcggtggt catcaagccc gacatggaaa aatatgtccg cgtcgggcgc gaaatacgcg
     7081 ccatgatgca ggcgctgacg ccgctggtcg agccgatttc catcgacgag gcattcctcg
     7141 acctgggcgg gaccgagaaa ttgcatgggc tgccgcctgc tctcgtattg gcgcgtttcg
     7201 tgcaggccgt ggaaaaggaa atcggcatca ccgtctcggc cggcctctcc tattgcaaat
     7261 tcctggccaa gatcgcgtcg gatttccgca agccgcgcgg cttctcggtg atcggccagg
     7321 ctgaggcggt cggttttctc gccgacaagc cggtcaccat gatctggggc gtcggcaagg
     7381 cattcgccgc caccctcgaa caagacggcg tccgcaccat cggccagttg caacgcatgg
     7441 aacgcggcga tctgctgcgc cgctacggca cgatgggcga ccggctctac cggctgtcac
     7501 gcggcgaaga cgagcgccgg gtggtttcag accgcgattc caaaagcgtg tcggccgaga
     7561 ccactttcga taccgatctg gcgagcgccg acgatctcgt gccggttttg cgggccttgt
     7621 ccgaaaaagt atccgcccgc ctgaaaaagg ccggcctgtc cgggcgcacc gtggtcttga
     7681 agctgaagac gcaggatttc aagaccagga cccgcaatcg ccagctcgaa gatccgacac
     7741 gccttgccga ccgcatcttc caaactggcc ttcacctgat cggcaaggaa ctcgacggaa
     7801 ccagatatcg gctgctcggc atcggcgtca gcgaccttgc cgattcgcag aaagccgatc
     7861 cgcccgatct ggtcgacgtg caggcccgca aacgcgccat ggccgaaggc gccatcgatg
     7921 cgctgcgcga caaattcggc ggcaaggcgg ttgaaacggg ctatacattc ggcaaaggcc
     7981 atcgcggccg ccccgcccgc ccggatgagg atgaaggccc cgaggtccag cacccctggg
     8041 agcgcatcta gagtatttcc agcaaaagtg tgaaacggtt ttgcgtccgg aaatgcgtcc
     8101 aaaacaaaga gatagagcgg ttttgtcgat tcagcaaaaa ctggaaccgc tccaggcgca
     8161 gggcgctggc cgccctggtc cagtcgttta acccttctag caattgcaga tcgccgcgcc
     8221 gcttgcgaga cagcaggcat gacacctgca ccctcgctca tcaaggccgc gctctggatg
     8281 gcgctatcca tcgcctcgtt tctgggcatg accgtcgcca gccgcgaaac gacgcgggtg
     8341 ttgaacgtgt tcgaagtcct ggaattgcgc tcggtgatcg gcctgctctt cctgatgcca
     8401 ctggtagtca ggagcggtgg ctttgccgcc atgcgaaccc agcgcccgtg gatgcatata
     8461 gggcgcaatt gcgcgcatta tgcaggccag ggggcatggc tctatgcgct gacgctcatc
     8521 ccgctggcgg ttctggtttc catcgaattc accacgccga tatggactgc ggtgctggcg
     8581 gtcagctttc tgggcgaaaa gctgagcaag gcgcgcgtgg catcgatcgt tttggggctg
     8641 atcggcgtta ccatcatcgt caggcccggt gtcggcgcgg taagcctggg cgaactggtg
     8701 gtgctggccg ccgccgtcag cttcggcgtt tcaatggtga tggtcaaatc gatgacgcga
     8761 acggaaagcg ttgtgcgcat catcttctgg atgctggtca tccaatcggc gctgggtttt
     8821 atcccggcct tggtccattg gcaaaatccg ccactagagc tatggccctg ggtgctgctc
     8881 atcggcttta ccggcatgtc gtcgcatttt ttcctggcgc gcgcgctgac ctatgcggat
     8941 gcaacgctga tctccccgat ggatttcctt cgcgtgcctg gaacagcact ggtcggctgg
     9001 gcgctctacg gcgaacagat cgacgtctac accgccaccg gcgccacgct gatcctgatg
     9061 ggaaacctgc tcaatcttca aaagcgcagg cgcgcgccca tcggggccgc gcaaacctaa
     9121 tcattattgt cgggaatgtt cagcagatgg ccgcaggcct tgcagtgaac cgcatccggc
     9181 tcgtgccgct gcaatccgca ttgcgggcat ggaaagaaga ctttgcttgg ccggaacacc
     9241 gattgcgcca gccgcacaaa cagcgagatg ccgatgatca tggtgacgat ggctgtcagc
     9301 ttgccggcca tgccgggcaa gacgatgtcg ccgaagccgg tcgtcgttac cgttgcgacg
     9361 gtaaaataaa gcgcatcgac ataaccggcc aggccggagc cgtcgcgaaa gaaaaacgtg
     9421 tagacgaaac cggtaactac cagcaggaac gtcaaaagat tcagaaccgc gtggcttgcc
     9481 tcgcgccagt ccctcaggcg gcgcatttcc agatgccgcc agagcacccc gctgcgcgac
     9541 agcgaccata gccgcaaaat gcgcaggaag ccgaaattgg ccagcgccgt cggaaacagc
     9601 agcgtcagca gaatgaagaa gtcgaccagc catgtcggct gcctcatcag ccgaagcatg
     9661 tcggtcgagg caagaagccg ggccagcatg tccgccgcca cgattgccgc caccagataa
     9721 tcgacccaga ggaacgaccg cgtttcctga agc
//
LOCUS       DASYCS010000121         5673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_751071,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000121 DASYCS010000000
VERSION     DASYCS010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 5673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5673
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751071"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..>5673)
                     /locus_tag="VGM46_04300"
     CDS             complement(25..>5673)
                     /locus_tag="VGM46_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinesin"
                     /protein_id="HEY4191845.1"
                     /translation="DRVMMIGQAVRREVAAMGEGIERTLARAVELETLVHSEVNQIER
                     SYTENEARIRTLVDGLGSEREAVVGHAERVRASIMGAHETLKDEIDSAGDSIRDSILN
                     ASTKLSMTITNSGDTLIDRINESSMSIFDSVEGRLDSITDRLSTSGEAFASLLDTRIA
                     KLTDSADGLTRSLTDLLDDRTAGMVSLLGGAARSLNTEFEASLNGIERTLAERGQALI
                     SEFQTRAEALDTGTQKLNAALEARARQINETLVERAREISNTFTQGKDTLSAAIDEGK
                     SQIHANMAEIVMSTSAMLEERAGDFAGRMEAARHVVSRSFDADIQRLGDARTGIDEAV
                     EEHSRKLAESGERMAAAMQADLAKFAEGRADIDAVVTGQVQKLAEGRDLISRALEEDL
                     RKVNESRAAIDVSLESHLTRLDEGRNRLSQALNEDTDNLVQARTIIDEMVAGHVGKLV
                     EGRSILTRALDADLDKLEQSKASIDGLVASQVEKIAEGRATLAKALESDIGGIRSLMD
                     NHSLKLAEDRSLLSQTLQEDIAKLAESRSSIDGLVAGQVEKLAEGRDILRRALEADLN
                     TIQGVIAGQSEKLMQDRSGFSQALEGDLEHVRGTVSNHMSRLADDRAALSRALDDDLT
                     KLAESRSSIDGLVAGQVEKLAEGRDILKRALEADLNTIQDVIAGQSEKLMQDRSGFSQ
                     ALEGDLERVRGTVSDHMNRLVDDRAALSRALDDDLTKLAESRSSIDGLVAGQVEKLAE
                     GRDILKRALEADLDTIQDVVADQSRKLVEERTEFSRALDADLQSVNGLVQEHMQRLAQ
                     DRATLGHALEDDLAKLADSRSSIDGLVAGQVEKLAEGRDILKRALQSDLDAIKGVVAD
                     QSEKLMEGHSLFSRSLEANLQSVNDLVHAQMQRLADDRNTLTGALEDDLTRLADSRAG
                     IDGLVAGQVEKLAEGRDILKRALEDDLAKLAETRRGIDLAVSGHIEQIAAKSTGISDF
                     IAADVQKIEAVFDRQTGIIEERAGTMERALSSGVDNIRGVLEKSAVFVAGQLREKVME
                     VTAALHEQAGEAFSDADRRIAERAEKTAAALLLRAEDIARTFEEADQRISTRAQDSSN
                     ALLSRAGETADELTARANAIADTFDMADQKLVARAVETAQSLAARAGDILRNFEDADQ
                     RLGARIGQSAEALAMRASDLGRIFDGADEKLTARITEGSEALSARVSEIGRVFDEADD
                     RMISRIASTSSSIGEHADHIVGAFSDTERRVAERVQQTGQGLAAHAREIEQALAGADE
                     RLANSAALTSARVEEHVAGVESRLSQTADTMGQKLSEQISQAENQLVSRANVIAETFT
                     AVGQHIGQSTNEAAKTIGQNTRELNSMLAARSAEITKILDETARPLVDRFAEGGAELQ
                     RSMEEVTQRATAKLREENSALVDALANRTAETLSAVEGARETLADSVHNLIGRMTASS
                     SQLNELIEHAAANLDQVDGRLSQSTQSFAATTDKAAQTFASSARLVDSNATRLSELSS
                     TTLREVASIATKFDEHSRLLSSASSLLGSAQSNLEHTLERQSSIEDLAVGLVKKSEDL
                     ERVMRSFENLVGQTLENAEGKTLQSADKIRAAISEVVESATKRFADATEEMRRTAGSI
                     KSELDLTRAELKKGVVEMPQEAKESTTAIRRAVSEQINALKELSDIVAKSGRGVDVAE
                     PRSLKPAQQPPVTRAPEPPRRAPAPAQPERRAPQAPVGGSPLRGSLEASPTAEAPARQ
                     REPNTRATPQGGWVQGLLAGASRDEAAQPAPVAASPAQRSPLHVVESLNSLSVDIARA
                     IDHDASIELWNRYRRGERDVFTRRLYTLKGQQTFDEIRRKYQTEAEFRAAVDRYCDDF
                     EKLLKDVSRNDRDNMITQTYLTSDTGKVYTMLAHASGRLH"
BASE COUNT          889 a         1782 c         1812 g         1190 t
ORIGIN      
        1 ccgacagtcg accttggtag tcggctaatg aagccgtcca ctggcatgag caagcatggt
       61 gtagaccttg cccgtatcgg acgtgagata ggtctgcgta atcatgttgt cgcggtcgtt
      121 gcgcgaaacg tccttcagca gcttttcaaa gtcgtcgcaa taacggtcga cggcagcgcg
      181 gaactcggct tccgtctggt acttgcggcg aatttcatcg aaggtctgct ggcctttcag
      241 tgtgtagaga cggcgcgtga acacgtcgcg ctcgccgcgc cggtagcggt tccacagctc
      301 gatcgacgca tcgtgatcga tggcgcgggc gatatcgacc gacagcgaat tcaacgattc
      361 cacaacatga agtggtgagc gctgggcagg cgatgccgca accggggcag gctgggcggc
      421 ttcgtcccgc gatgcgccgg ccagcaaacc ctggacccag ccgccctgag gcgtggcacg
      481 cgtattcggc tcgcgttgac gcgcgggcgc ctccgccgtc gggctggctt ccagcgagcc
      541 acgcagcggg ctgcccccaa ccggcgcctg cggagcacgg cgctccggtt gtgcgggagc
      601 cggagcgcgc cgcggcggtt ccggtgcgcg agtgacgggc ggctgctgag ccggcttcaa
      661 ggaacgcggc tcggcaacgt cgacgccacg gccagatttt gcaacgatat cagatagttc
      721 cttcaaggcg ttgatctgct ccgagacggc gcggcggatt gccgtggtcg actcttttgc
      781 ttcctgtggc atctccacca cgcccttttt cagttcggcg cgggtgaggt cgagttcgct
      841 cttgatggag ccggccgtgc ggcgcatttc ctcggtcgcg tcggcaaagc gcttggtggc
      901 ggactcgacc acctcgctga tggcggcgcg aatcttgtcg gccgattgca gcgtcttgcc
      961 ttccgcattc tccagggtct ggccaacgag gttctcgaac gaacgcatca cccgctcaag
     1021 gtcttccgac ttcttgacga ggccgacggc gagatcctcg atcgaggact gacgttccag
     1081 cgtatgttcg aggttgctct gcgcggaacc cagcaggctg gatgccgaag aaaggaggcg
     1141 gctgtgctcg tcgaacttgg ttgcgatgga tgccacttcg cgcagcgtgg tggacgaaag
     1201 ctcgctcagc cgtgtcgcgt tggaatcgac caggcgcgcc gagctggcga aagtctgcgc
     1261 cgccttgtcg gtcgtcgcag cgaagctttg cgtgctttgc gacagacggc catccacttg
     1321 atcgagattg gccgctgcat gttcgatcaa ctcgttgagc tgcgaactgg aagcggtcat
     1381 ccggccgatc aaattatgaa cgctgtcggc cagcgtttcg cgcgcacctt ctaccgccga
     1441 caaggtttcg gcggtgcggt tggcgagcgc atcgaccaga gccgaatttt cttcgcgcag
     1501 cttggcggtg gcgcgctggg tcacctcttc catgctgcgc tgcaactcgg cgccaccttc
     1561 ggcgaagcgg tcgaccagcg gtcgcgccgt ctcatcgagg atcttggtga tttcggccga
     1621 acgggccgcg agcatggagt tcagctcacg cgtgttctgc ccgatggtct tggcggcctc
     1681 gttggtgctt tggccgatat gctggcccac cgccgtaaac gtttcggcga tgacgttggc
     1741 gcgcgagacc agctggtttt cagcttgcga gatttgttcg ctgagcttct ggcccattgt
     1801 gtcggcggtt tgcgaaagcc tgctctcgac gccggcaacg tgctcttcga cgcgcgctga
     1861 cgtcaaagcc gcgctgttgg cgaggcgctc gtccgctccg gccagcgcct gctcgatttc
     1921 gcgggcatgg gctgcaagcc cctgtccggt ctgctggaca cgctccgcga cccgccgttc
     1981 cgtatcggaa aaggcaccga cgatatgatc ggcatgttcg ccgatcgagg aggatgtcga
     2041 ggcgatgcgc gaaatcatgc ggtcgtcggc ttcatcgaat acgcgcccga tttcgctcac
     2101 cctggcggaa agcgcttccg agccttcggt gatgcgcgcg gtcagtttct cgtcggcgcc
     2161 gtcgaagatg cggccgagat ccgaagcgcg catggccagc gcttcggcgg attgcccgat
     2221 gcgcgcgcca agcctttgat cggcgtcctc gaaattgcgc aggatgtcgc ctgcgcgggc
     2281 ggccagcgat tgcgccgttt caacggcccg agctaccagc ttctggtcgg ccatgtcgaa
     2341 cgtatcggcg attgcgttgg cgcgcgccgt cagttcgtcg gcggtttctc cggcgcgcga
     2401 aagcagggcg ttggaactgt cctgtgcgcg tgtcgaaatc cgctggtccg cttcctcgaa
     2461 ggtgcgggcg atgtcctcgg cccgcagcag aagggcggcg gccgtctttt cggcgcgctc
     2521 ggcaatcctg cggtcggcgt cgctgaaggc ttcgcctgcc tgctcgtgca atgccgcggt
     2581 gacttccatc accttttcgc gcaactggcc ggccacgaat acggcgctct tttcgagcac
     2641 gccacgaatg ttgtcgacac cggaagacag tgcgcgctcc atggtgccgg cgcgttcctc
     2701 gatgatgccg gtctggcgat cgaacacagc ctcgatcttt tgcacgtctg cggcgataaa
     2761 gtcggaaatc cccgtgcttt tggcggcgat ctgctcgata tggccggata cggcaagatc
     2821 gatgccccgg cgcgtttccg cgagctttgc aaggtcgtct tccagcgcgc gcttgaggat
     2881 atcgcggcct tcggcaagct tttccacctg tccggcgacc agcccgtcaa tgccggcgcg
     2941 gctgtcggcc agccgcgtga gatcgtcttc cagggcgccg gtgagggtgt tgcggtcgtc
     3001 cgccagccgt tgcatctggg catggacgag gtcgttgacg ctttgaagat tggcttccag
     3061 tgaacgagaa aacagcgaat ggccttccat cagcttttcg gactggtcgg caacgacgcc
     3121 cttgatggcg tcgagatcgg attgcaacgc ccgcttgagg atatcgcggc cttcggccaa
     3181 cttttccacc tgtccggcaa ccagtccgtc gatgctggag cggctgtcgg ccagcttggc
     3241 caggtcgtct tcgagcgcat ggccaagcgt cgcccgatcc tgggcgagcc tttgcatatg
     3301 ttcctgcacg aggccgttga cgctttgaag gtcggcgtcc agcgcccgcg aaaattccgt
     3361 ccgctcttcc accagctttc gcgattgatc ggcgaccacg tcctggatgg tgtccaggtc
     3421 cgcctccaaa gcgcgcttga ggatatcgcg gccttcggcc agcttttcca cctgtccggc
     3481 caccagcccg tcgatgctgg aacggctttc ggcgagcttg gtcaggtcgt cgtccagcgc
     3541 ccgcgaaagg gctgcgcgat cgtcgaccag cctgttcata tgatcggaga ccgtgccgcg
     3601 aacgcgttcg agatcgcctt ccagcgcctg cgagaagccg gaacggtcct gcatcagctt
     3661 ttcggattgc ccggcaatga cgtcctggat ggtgttcagg tcggcttcca aagcgcgttt
     3721 cagtatatcg cggccttcgg ccaacttctc gacctgtccg gcgaccagcc cgtcgatgct
     3781 ggagcggctt tcggccagct tggtcaggtc gtcgtccagc gcccgcgaaa gggcggcgcg
     3841 gtcgtcggcc agcctgctca tatgattgga gaccgtgccg cgaacatgct caaggtcgcc
     3901 ttccagcgcc tgcgagaaac cggaacggtc ttgcatgagc ttttcggact ggccggcaat
     3961 gacgccctgg atggtgttca ggtcggcttc cagcgcgcgc cggagaatgt cgcggccttc
     4021 ggccagcttc tcgacctgtc cggcgaccag accgtcaatg ctggagcggc tttcggccag
     4081 cttggcgatg tcttcctgca acgtttgcga gagcaggctg cgatcttcgg cgagcttgag
     4141 agaatggttg tccatgagcg agcgaatgcc gccgatgtcg ctctccagcg ccttggccag
     4201 cgtagcgcgg ccttcggcga tcttttcgac ctggctggcc accagcccat caatgctggc
     4261 cttgctttgc tccaatttgt cgaggtcggc atcgagcgcg cgggtcagga tgctacgccc
     4321 ctcgaccagt ttcccgacat gaccggcgac catttcatca atgatcgtac gggcttgcac
     4381 cagattgtcg gtatcttcat tcaaggcctg cgacagccgg ttgcgaccct cgtccaggcg
     4441 cgtgagatgg ctttccagtg agacgtcgat ggctgcgcgc gattcgttga ccttgcgcaa
     4501 atcttcttct agggcacgcg agatgaggtc gcggccctcg gccagcttct gcacctggcc
     4561 agtcaccacg gcgtcgatgt cggcgcggcc ttccgcaaac ttggccagat cggcctgcat
     4621 cgcagcggcc atgcgctcgc cgctctcagc cagcttgcgg ctgtgttcct cgacggcttc
     4681 atcgataccg gtgcgagcat cgcccaggcg ctggatatcg gcgtcgaacg agcgcgacac
     4741 gacatgacgc gccgcttcca tccgtccggc aaagtcgccg gcgcgctctt cgagcatggc
     4801 cgatgtcgac atgacgattt ccgccatgtt ggcatggatc tggcttttgc cttcatcgat
     4861 ggcggcggac agcgtgtcct tgccctgcgt aaaggtgttg gagatttcac gggcgcgctc
     4921 gaccagcgtt tcgttgatct ggcgggcacg cgcttccagc gcggcattca gcttctgcgt
     4981 accggtatcg agcgcttcgg cgcgggtctg gaattcgctg atgagcgcct ggccgcgctc
     5041 ggccagcgtc cgctcgatgc cgttcaggct tgcttcaaat tcggtgttca ggctgcgcgc
     5101 cgcacccccc agaagcgaca ccatgccggc tgtgcggtcg tccagaaggt cggtcagcga
     5161 gcgggtaagt ccgtcggcgc tgtcggtcag cttggcgatg cgggtatcca gcaggctggc
     5221 gaacgcctcg ccggaagtcg aaagccggtc ggtgatggaa tcgaggcggc cttccacgga
     5281 atcgaagatc gacatcgaac tttcattgat gcggtcgatc agcgtatcgc cggaattggt
     5341 gatcgtcatc gacagcttcg tcgatgcatt caaaatgctg tcgcgaatgg aatcgccggc
     5401 ggaatcgatc tcgtccttca gcgtctcgtg tgcacccatg attgaggcgc gcacacgctc
     5461 cgcatggccg acgaccgctt cgcgctcgct gccaagacca tccaccagcg tgcggatgcg
     5521 ggcctcgttc tccgtatagg agcgctcgat ctggttgact tcgctatgta ccagcgtttc
     5581 aagctcgacg gcgcgcgcca gcgtgcgctc gatgccttcg cccatcgccg ccacttcgcg
     5641 gcgaacggcc tggccaatca tcatcacccg atc
//
LOCUS       DASYCS010000122         2292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4667145,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000122 DASYCS010000000
VERSION     DASYCS010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4667145"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(298..1518)
                     /locus_tag="VGM46_04305"
     CDS             complement(298..1518)
                     /locus_tag="VGM46_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006204812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY4191846.1"
                     /translation="MTAGPAPFGTTQALDIDADIAVIGAGLAGTLAAIVLGRAGHRVA
                     LIDRNAVCPPEFRVEKIGGEQIAAMQRLGLLDALSAHATRFDEIVNARKGRILDRTRS
                     VHFGFRYADIVSAMRRQLPQSVRFVVGRVDAVTTGADRQAVTIAGQGTVNARLVVLAT
                     GMGDLLRRTLGIGREVIHQRQSLTFGFDIAPAPGASFENPALTYYGERLSDGIDYLSI
                     FPVDGATRANLFTFRDIGDPWVKAFRQNPKETLAATLPGLARVLGDFEIVSPVQNWLM
                     DIAVAQDYRQAGVVLIGDAYQTSCPAAGTGVARLLSDVERLCMVHIPNWQASPGMSAD
                     KIGEFYADPHKQAADARALQMARYRRSLTVETDLRWRAHRMLQLSRRRLMSGIERYTP
                     SVATRLRMLRARHA"
     gene            complement(1515..2291)
                     /locus_tag="VGM46_04310"
     CDS             complement(1515..2291)
                     /locus_tag="VGM46_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEY4191847.1"
                     /translation="MQSFEEVLAEVPTVVVGGMPVSIADRQTTAALMIRAGKERSRGG
                     RPLYFTSVNGETIARANADKAISALFRNADHIAADGQPLVLASRWLCRHRLPERVATT
                     DLFHDVAKLAQENGSTFYLLGATEPENIRAVAVIEAAYPALKIVGHSHGYLTGKALAA
                     KLDEIDALAPDILWLGLGVPLEQVFVRDFSTSLPNVGIIKTAGGLFNHLSGKNPRAPG
                     WMQKAGFEWLWRSLIEPRRLVWRYLTTNPRAIYAILRYSK"
BASE COUNT          420 a          727 c          761 g          384 t
ORIGIN      
        1 gtggcagatc tcgcgctcgg gcagcccgct cggctttgcc gccggccttg tggtggaaag
       61 actgccggca gcgaagaagc tggcgcgccg gatgtttcac ggcgcaaaag acgcacagcc
      121 cgctgcccgc gccgcgccgg cggccgccat cgccgaagaa gcaaccgttt cctagaacgg
      181 ctctggtttt aaccgagccg gtaaaaccac tctatctctt tgttttggcc gcatttccga
      241 acggaaagcc gtttcacgct ttcgctggaa atgctccaga ttacagtctg ccgcgcctca
      301 ggcgtggcgg gcgcgcagca tgcgcagccg tgtcgcgacc gagggcgtat agcgctcgat
      361 gcccgacatc agccggcggc gcgacagttg cagcatgcga tgggcgcgcc agcgcagatc
      421 ggtttcaacc gtcaggctgc ggcgataacg cgccatctga agcgcgcgcg cgtcggctgc
      481 ctgcttgtga gggtcggcat agaactcgcc gatcttgtcc gccgacattc cagggctcgc
      541 ctgccagttc gggatgtgga ccatgcacag ccgctcgaca tcgctgagca gacgggcaac
      601 gccggtgccc gccgccgggc aggaagtctg ataggcatcg ccgatgagca caacgccggc
      661 ctgccggtaa tcttgcgcga ccgcgatatc catcagccag ttctgcaccg ggctgacgat
      721 ttcgaaatcg ccgagaacgc gcgccaggcc gggcagcgtc gcggccagcg tttccttcgg
      781 attctggcga aacgccttga cccatggatc gccgatgtcg cggaaggtga acaggttcgc
      841 ccgcgtcgcg ccatccaccg ggaaaatgct gagatagtcg atgccgtccg aaagacgctc
      901 gccataatag gtcagcgcgg ggttttcaaa ggaagcgccc ggcgccgggg caatatcaaa
      961 gccgaaggta agcgactggc gctgatggat gacttcgcgc ccgatgccca gcgtgcgccg
     1021 cagcaggtcg cccatgcccg tcgccagcac gacgagacgc gcgttgaccg tgccctgccc
     1081 ggcgatggtg accgcctggc ggtccgcgcc tgtggtcacg gcatcgacgc gcccgacgac
     1141 aaagcggacg ctttgcggca attgccggcg catggcggag acgatatcgg catagcggaa
     1201 gccgaaatgc actgaccggg tcctgtccag aatacggccc ttgcgggcgt tgacgatttc
     1261 atcgaagcgg gtggcgtgcg ccgacagcgc gtcgagcagg cccagacgct gcatcgccgc
     1321 aatttgctcg ccgccgattt tctcgacccg gaattccggc ggacaaaccg cgttgcggtc
     1381 gatcagcgcg acacggtgac cggcgcggcc gagcacgatg gcggcaagcg tgccggcgag
     1441 gcccgcgccg atgaccgcga tatcggcatc gatatcgagc gcctgcgtcg tgccgaaggg
     1501 agccgggccg gccgtcattt ggaatacctc aatatggcat agatggcgcg cggattggtg
     1561 gtcagataac gccagacgag gcggcgtggc tcgatcaggg agcgccacag ccactcgaag
     1621 ccggccttct gcatccagcc gggcgccctg gggttcttgc cggagagatg gttgaacagg
     1681 ccgcccgccg tcttgatgat gccgacgttc ggcaggctgg tcgagaaatc gcgcacgaag
     1741 acctgctcaa gcggtacgcc gagaccaagc catagaatgt cgggggcaag cgcgtcgatc
     1801 tcgtcgagct tggcggcaag tgccttaccg gtgagatagc cgtgggaatg accgacgatt
     1861 ttcaatgccg gatatgccgc ctcgatgacg gcgaccgccc ggatgttttc cggttccgtc
     1921 gcgccaagca gatagaaggt cgagccgttt tcctgcgcga gcttggccac gtcgtggaac
     1981 aggtcggtgg tcgccacccg ctcgggaagc cggtgacggc acagccagcg cgatgcaagc
     2041 acgagcggct ggccatcggc ggcgatatgg tcggcattgc gaaacagcgc cgaaatggcc
     2101 ttgtcggcgt tggcgcgggc gatggtttcg ccgttcaccg aagtgaaata cagcggcctg
     2161 ccgccgcggc tgcgttcctt gccggcgcgg atcatcaacg ccgctgttgt ctgccggtcg
     2221 gcgatactga ccggcatacc gccgaccacc accgttggaa cttcggccag gacttcttca
     2281 aacgactgca ac
//
LOCUS       DASYCS010000123         2809 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3072744,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000123 DASYCS010000000
VERSION     DASYCS010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3072744"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VGM46_04315"
     CDS             <1..376
                     /locus_tag="VGM46_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002966589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY4191848.1"
                     /translation="GMTAWLPSADLKDRWFGNAAAFAKDYKAKFGYDPDYHAASGAAD
                     VEALAKAIEDAGGTDPAKVRDALAALKLDSLYGKIAFAGNGQIDLPQTVIQVQGGEVA
                     AIYDANGFIEKPKYPMPAWNAR"
     gene            421..1284
                     /locus_tag="VGM46_04320"
     CDS             421..1284
                     /locus_tag="VGM46_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009363936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY4191849.1"
                     /translation="MDLLAQILINGVLLGGLYAIMALGIALVWGVLNIVNLAHGAFIM
                     LGAYLSWHLYTYLGIDPFLGLPITAIIMFLFGYGVQRGLLNLVVRGPMFNTLLITFGL
                     EVVFTYLAQLAFSADFRTINPAYAGNSIAFGPVVLPLARLMAFGVAIVLTVGMWLFLL
                     HSRLGRAIRATAQNLVAARLYGVEPRHLYAMTFGIGIGLAGAAGGLYGTVSQINPYIG
                     ATLTAKCFAIAIIGGLDNPLGVIVGGLVLGIIESLAVLYIGATFADVASFGVLVLVLI
                     VRPSGLLGKTA"
     gene            1281..2228
                     /locus_tag="VGM46_04325"
     CDS             1281..2228
                     /locus_tag="VGM46_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY4191850.1"
                     /translation="MTAARIAFIVLAIVVLAAVPWFGSDVLVQFGINALLLAVLAQGW
                     NIIGGYAGYASFGNSVFYGLGSYGVAIAMVQWELPFAFGLAFGVVMAVVFAFLLGLPV
                     LRLKGHYFAIATLALAQVMIAIVSNVGLAGQNIGLVLPPLNNDPLFYELSLGLLVVTT
                     LTIFWLTRSRFGFGLIAIRENEEGAAVMGVNTTLYKVLAFALSGVFSALAGGIHAYWI
                     TFLDPESAFDISLNVKMIIMAVFGGPGTVLGPVVGALTLTTVSEFLSSEVTSVAGLFF
                     GIVVVAAVVLMPRGLADVVRRFRKSGWRYFAENIKAHRI"
     gene            2225..>2809
                     /locus_tag="VGM46_04330"
     CDS             2225..>2809
                     /locus_tag="VGM46_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004687607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4191851.1"
                     /translation="MSAILEVNHVTRRFAGLIAVNDVSFTLGQGEILGVIGPNGAGKT
                     TLVSLISGTLAPSSGDIAFEGKAIATLPAFRRARLGIGRTFQIMRPFPGLSVLDNVAV
                     GALFGRGEGAAGLPEAREKARAHLDFVGLGKAVDQRADELGGPGRKRLELAKALAMQP
                     KLLLCDEVMAGLNHVEIDEVIEVIRKVRGEGISVL"
BASE COUNT          432 a          907 c          897 g          573 t
ORIGIN      
        1 cggcatgacg gcctggctgc cgtccgccga cctcaaggac aggtggttcg gcaatgccgc
       61 cgccttcgcc aaggactaca aggcgaagtt cggctacgat cccgattacc atgccgcctc
      121 cggcgcggcg gacgtcgaag ccctggccaa ggccatcgag gatgccggcg gcaccgatcc
      181 ggcgaaagtg cgcgatgcgc tggcggcgct caagctcgac agcctctacg gcaagatcgc
      241 cttcgccggc aacggccaga tcgacttgcc gcagaccgtc attcaggtcc agggcggcga
      301 agttgcggca atctacgacg ccaacggctt catcgagaag ccgaaatacc cgatgccggc
      361 ttggaacgcc cgctgaacag gccggctgac ccggtttcgg gcaaataaag ggaggacggg
      421 gtggatctgc tcgcgcaaat cctgatcaac ggcgttctcc tcggagggct gtacgccatc
      481 atggcgctgg ggatcgccct tgtctggggc gtgcttaaca tcgtcaatct ggcgcacggc
      541 gccttcatca tgcttggcgc ctacctgtca tggcatcttt atacctatct cggcatcgac
      601 ccgttcctcg ggctgccgat caccgcgatc atcatgttcc tgttcggcta tggcgtgcag
      661 cgcgggctgc tcaaccttgt cgttcgcgga ccgatgttca acacgctgtt gatcaccttc
      721 ggccttgaag tcgtgttcac ctatctggcg cagctggcct tttcggccga cttccgcacc
      781 atcaatccgg cctatgccgg caatagcatc gcgttcggcc ctgtggtgct gccgctggcg
      841 cggttgatgg ccttcggtgt cgccatcgtg ctgacggtcg gcatgtggct gttcctgctg
      901 cacagccggc ttggccgcgc catccgcgcg acggcgcaaa acctggtggc cgcgcggctt
      961 tacggcgtcg agccgcgcca cctctacgcc atgactttcg gcatcggcat cgggctggcc
     1021 ggggctgccg gcgggcttta cggcacggtc tcgcaaatca acccctatat cggcgcgacg
     1081 cttaccgcca agtgctttgc catcgccatc atcggcggcc tcgacaatcc gcttggcgtc
     1141 atcgtcggcg gtcttgtcct cggcatcatc gaatcgctgg ccgtgctcta tatcggcgcg
     1201 acattcgccg acgtggcgag cttcggtgtg ctggtgctgg tgctgatcgt acggccaagc
     1261 ggcctgttgg ggaaaaccgc atgaccgccg cgcggattgc cttcatcgtt ctcgccatcg
     1321 tcgtgcttgc ggcggtgccg tggttcggct cggacgtgct ggtgcagttc ggcatcaatg
     1381 cgcttttgct ggccgtcctg gcgcagggct ggaacatcat cggcggctat gcgggctatg
     1441 cctccttcgg caattcggtc ttttacgggc tgggcagcta cggcgtggcg atcgccatgg
     1501 tgcaatggga actgcccttc gccttcggcc ttgccttcgg cgtggtaatg gcggtggtct
     1561 tcgctttcct gctcggcctg cccgtgctgc ggctcaaagg ccattacttt gccatcgcaa
     1621 cgcttgccct ggcgcaggtg atgatcgcca tcgtctccaa tgtcggcctg gccggccaga
     1681 acatcggcct ggtgctgccg ccgctcaaca acgatccgct gttctacgaa ttgtcgctcg
     1741 gccttctggt ggtgacgacg ctgaccatct tctggctgac gcgcagccgg ttcggcttcg
     1801 ggctgatcgc catccgcgaa aacgaagagg gtgcagcggt gatgggcgtc aacaccacgc
     1861 tttacaaggt tctcgccttt gcgctgtcgg gcgtgttcag cgcgcttgcc ggcggcatcc
     1921 acgcctattg gatcaccttc ctcgatcccg aaagcgcctt cgacatcagc ctcaacgtca
     1981 agatgatcat catggccgtg ttcggcgggc cgggaacggt gctgggaccg gtcgtcggcg
     2041 cgctgacgct caccaccgtt tcggaattcc tgtcgagcga ggtaaccagc gtcgcgggcc
     2101 tgttcttcgg catcgtggtc gtcgctgccg tcgtgctgat gccgagggga ttggccgatg
     2161 tggtgcgccg cttccgcaag tcggggtggc gctacttcgc cgaaaacatc aaggcccacc
     2221 ggatatgagc gcgatcctgg aggtaaacca cgtaacgcgg cgctttgccg ggctgatcgc
     2281 ggtcaacgac gtttcgttca cgcttggcca gggcgagata ctcggcgtca tcggtcccaa
     2341 cggcgccggc aagacgacgc tggtgagcct gatcagcgga acgctggcgc cgagttccgg
     2401 cgacatcgcc ttcgaaggca aggccattgc cacccttccc gcattccggc gggcgcgact
     2461 gggcatcggc cgcacctttc agatcatgcg gcccttcccc ggtctttcgg tgctcgacaa
     2521 cgtcgccgta ggcgcgctgt tcgggcgcgg cgaaggcgct gccggactgc ccgaggcgcg
     2581 cgaaaaagca cgcgcccatc tcgatttcgt cgggctgggc aaggccgtcg accagcgcgc
     2641 cgacgaactt ggcgggccgg gccgcaagcg cctcgaactg gccaaggcgc tcgccatgca
     2701 gccgaagctt ctgctgtgcg acgaggtgat ggccggcttg aaccatgtcg agatagacga
     2761 ggtcatcgag gtcatccgca aggtgcgcgg cgaaggcatt tcggtgctg
//
LOCUS       DASYCS010000124         5690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_172606,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000124 DASYCS010000000
VERSION     DASYCS010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 5690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5690
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172606"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /gene="gmd"
                     /locus_tag="VGM46_04335"
     CDS             complement(<1..1043)
                     /gene="gmd"
                     /locus_tag="VGM46_04335"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006330750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEY4191852.1"
                     /translation="MKRPVALITGITGQDGAYLSELLLAKGYEVHGIKRRSSSFNTGR
                     IDHLYQDPHERDVRLTLHYGDLTDATNLIRVVQEVQPDEIYNLAAQSHVQVSFDTAEY
                     TANADALGTLRLLEAVRLLKLRDKTRFYQASTSELYGKVQAVPQSETTPFYPRSPYAA
                     AKLYAYWMVVNYREAHGLHASNGILFNHESPVRGETFVTRKITRAAAAISLGLQDCLY
                     LGNLDAERDWGHARDYVEGMWRMLQQEKPDDYVLATGKKHSVRAFVETAFSEIDVAIE
                     WTGRGVEEVGRDAKTGKKIVAIDPRYFRPTEVDLLLGDPRKAKRVLGWSATVTFEAMV
                     REMMRSDVDTVAR"
     gene            complement(1110..3314)
                     /locus_tag="VGM46_04340"
     CDS             complement(1110..3314)
                     /locus_tag="VGM46_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011424687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HEY4191853.1"
                     /translation="MTVLMPTTDSVTRSLIGHEPPQPFDSGPDELDLSAGLRLLRRRI
                     GMIAAITGLLTLACIPLILGLKPTYHAESRLMIYSPLASTLGTGDAGQGSALNINSET
                     ERLYSRPIAQRVIADLGLADLEEFNPALREASILGKLRNAMRGLVDGGAAAAGDSDGI
                     ERIIPEYYQALNIRRDGVSDVIQIGFNSRDPKLAAAVPNKILGVYLDEREAGTKGRFS
                     SAEEWIRQRLADQKARVNAAREAAARYGKAVGAVSNDAQAEQIRAVADLNGRSAKIVQ
                     GKAELAATISALEAAGSDGDIGKIAVPEGIGSLQRDLRALDEELDQLLLTYGNNAEEV
                     IDQRAKISKAKTDLNFEIGRYLQAQRAKLAGFSQQERSVKSALVVAREQLSRSTTAQT
                     ELTHLVRVADMEQAAFDKLEDQNRALVAQAALPAVEVEMLSPATVPLQPQGRGRLFYL
                     IGAMLAAISIATTVAILREMMDKSVRSHEQLQAIADIVPVGQVPALSERAGKTMPMVF
                     GDSEGGVFAEAVRSMIMALKQVHGGKLPQSIMVTSAHGGEGKTLIARSLAIELVASGH
                     KVLLVDGDLRGGDLGTLFKSGVKVGLNEFLTGQAGLGDIVYHHAKSGIDFIPRGNPSL
                     NRRPHLPDMQEIVKLAKENGQILVLDSASLLSSTDTVYLASLAERSILVVQWAQTTHR
                     AVEAAAQRLRNIRRSEILIAINNINIRRHALYGFKDSEMFMDTTKRFYAISA"
     gene            complement(3311..4486)
                     /locus_tag="VGM46_04345"
     CDS             complement(3311..4486)
                     /locus_tag="VGM46_04345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018493935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY4191854.1"
                     /translation="MRVKLNAADFSAGVAIAAERARASGRVEAPVRHYVAGGRENGGG
                     IGRLVGYIADTSAEAGIPHLVTDTRGPRWFAPTSLMRLSAAILLMAKDRLLVPERIHH
                     IHIAGRGSTSRKLILTAAARALSCRHVLHLHDYDYANDFSVRPPRQKKRIRAMFQSAD
                     RVVALGRRDSATLSEVLGVDPARIIVAHNCVPDPGPHPVRIDESPLILFLGRLGERKG
                     VPELLAALGSPVMADLPWRAVLAGDGPVSEYRRQASNMGLSDRVTMPGWIDLKGTRAL
                     CARADILVLPSHAEGMAMAVIEGLAHGLAVVTTRVGAHEEAISDGETGLFVPVGDKDA
                     LALALARLVGDPWMRGALSSKARSHYLSRFSMVAYGRRLEKLYEAVSSGKQTRVHAQ"
     gene            complement(4477..5580)
                     /locus_tag="VGM46_04350"
     CDS             complement(4477..5580)
                     /locus_tag="VGM46_04350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018483893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY4191855.1"
                     /translation="MKALLLVADLEDYTISFVNGVARHMPVVLGVPRRRYGHLASWFD
                     PAVDLRLMDWPRHSALSNPWFLFKLTRLIRRERPSLIHLLSNTTLWLNFAAPFWRPIP
                     LVTTVHDVRVHPGDVETSVLPNWAPTLIVRQSGHVVVHGDGLKQMAIDRFSKSPDRVH
                     VLSHPSIVRYCDLARREKLVRRPDDGTMRVLMFGRIFAYKGLDQLIRAEALLKDAIPN
                     LRVTIAGRGDDPWTLRSLMGEPSRYDIQNRFIEDVEVAQLFLDCDMVVLPYAEASQSG
                     VLNLAAAFGKPVIVTDVGELRATVEPNGLGMVVPPGDPEMLARAIRILAESVTLRTGL
                     GANALAWATGPNAPEAVGARAACLYRTVVQSCA"
BASE COUNT         1039 a         1796 c         1830 g         1025 t
ORIGIN      
        1 tcgcgcgcga cggtatcgac atcggagcgc atcatctcgc gcaccatcgc ctcgaaggta
       61 accgtggccg accagccgag gacgcgtttt gccttgcgcg ggtcgcccag aagcaggtcg
      121 acttcggttg gcctgaaata gcgtggatcg atggccacga tcttcttgcc ggtttttgca
      181 tcccgaccga cttcctcgac gccccggcct gtccattcga tggcgacatc gatctcggag
      241 aacgcggttt cgacgaaggc gcgaaccgaa tgtttcttgc cggtggccag cacataatcg
      301 tccggctttt cctgctggag catgcgccac atgccctcga cgtaatcgcg cgcatggccc
      361 cagtcgcgct cggcatcgag attgccgaga tagaggcaat cctgcaagcc gagcgaaatc
      421 gcggccgcgg cccgcgtgat cttgcgggtg acgaaggttt cgccgcgaac tgggctttca
      481 tggttgaaca gaatgccgtt ggaggcgtgc aggccgtgcg cctcgcggta attgaccacc
      541 atccaatagg cgtaaagctt ggccgccgca tagggcgagc gcggatagaa cggcgtggtt
      601 tcggattgcg ggaccgcctg caccttgccg taaagctccg acgtcgacgc ctggtagaag
      661 cgggtcttgt cgcgcaattt cagcaggcgg acagcctcca gcaggcgcag cgtacccagc
      721 gcatcggcgt ttgccgtata ttcagccgtg tcgaagctga cctggacatg gctttgcgcg
      781 gcgagattgt aaatctcgtc aggctgaacc tcctgcacga cgcggatcag gttggtcgcg
      841 tcagtgaggt cgccgtaatg cagcgtcagg cgcacgtcgc gttcatgcgg gtcctggtag
      901 agatggtcga tacggccggt attgaaggac gaggatcgcc gcttgatgcc gtgaacctca
      961 tagcccttgg ccagcagaag ttcggaaaga taggcgccat cctggccggt gatgccggta
     1021 atcagggcta cgggtctctt catgtgacgc ctcccagcgt gaacggtatt cggtaatttt
     1081 ccatgcatgg ctgtcggctt ccggcaaaat catgccgaga tggcgtagaa gcgcttcgtc
     1141 gtgtccatga acatctcgga gtccttgaac ccgtacaaag cgtgcctgcg tatgttgatg
     1201 ttgttgatgg cgatcagtat ctccgagcgc cttatgttgc gcaggcgctg ggccgccgcc
     1261 tcgacggcgc ggtgcgttgt ctgcgcccat tgcacgacaa ggatggaccg ttcggcgagg
     1321 cttgccagat agacggtatc ggtcgaggac agcagcgaag cgctgtcgag aacaaggatc
     1381 tgcccgtttt ccttcgccag cttgacgatt tcctgcatat ccgggagatg cgggcgacgg
     1441 ttcaggctcg gattgccgcg cggaatgaag tcgataccac tcttggcatg gtggtagaca
     1501 atgtcgccca atccggcctg gccggtcagg aattcgttca gccccacttt cacgcccgac
     1561 ttgaagagcg tgccgaggtc gccgccgcgc aggtcgccat ccaccagcag caccttgtgg
     1621 ccggaggcaa cgagctcgat ggccagcgag cgcgcaatca acgtcttgcc ctcgccgcca
     1681 tgggctgagg tgaccataat actctgcggc agctttccgc catggacctg cttcaaggcc
     1741 atgatcatcg agcggaccgc ctcggcgaac acgccgcctt cactatcgcc gaacaccatc
     1801 ggcatggttt tcccggcgcg ttcgctcaag gccggcacct ggccgacggg tacgatgtcg
     1861 gcaatcgcct gcaactgctc gtggctgcga acggacttgt ccatcatctc gcgcaggatg
     1921 gccacggtcg tcgcaatcga gatcgccgcc agcatcgcgc cgatcaggta gaacagccgc
     1981 ccgcgcccct gcggttgcag cgggacggtg gcgggagaaa gcatttcgac ctcgacggcc
     2041 ggcagcgccg cctgggcgac caaggcgcgg ttttgatcct caagcttgtc gaaggccgcc
     2101 tgctccatgt ccgcgacgcg cacgaggtgc gtcagctcgg tctgcgccgt ggtggaacgg
     2161 gaaagctgtt cgcgcgcgac gaccagggcg ctcttcaccg aacgctcctg ctggctgaag
     2221 ccggccagct tggctcgctg cgcctgaaga tagcgcccga tctcgaaatt cagatcggtc
     2281 ttcgcctttg atatcttggc gcgctggtcg attacttcct cggcattgtt gccgtaggtc
     2341 aaaagaagct ggtcaagttc ctcgtcgagc gcgcgaaggt cgcgttgcag cgagcctatg
     2401 ccctcgggaa ccgcgatctt gccgatgtcg ccgtcgctgc cggccgcttc cagcgccgaa
     2461 atggtcgccg ccagttccgc cttgccctgc acgatcttgg cggagcggcc attcaaatcc
     2521 gcaacggccc tgatctgctc ggcctgggcg tcgttggata ccgcgccgac cgccttgccg
     2581 tagcgcgcgg ccgcttccct tgctgcattg acgcgggctt tttggtcggc gaggcgctgg
     2641 cggatccatt cctcggcgga agagaaccgc cccttcgtgc cggcctcgcg ctcatcgaga
     2701 tagacgccga gtatcttgtt tggaaccgct gccgcaagtt tcggatcgcg cgaattgaac
     2761 ccgatctgga tgacatcgga aacgccgtcg cgccggatgt tcaaagcctg gtaatattcg
     2821 ggaatgatcc gctcgatgcc atcggaatcg ccggccgccg ctgctccgcc atcgacgagg
     2881 ccgcgcatcg cattgcgcag cttgccgaga atcgacgcct cccgcagcgc cgggttgaac
     2941 tcttccaggt cggcgaggcc aaggtcggca atgacccgtt gcgcaatagg cctggagtaa
     3001 agccgctccg tctcggaatt gatgttcagc gcgctgccct ggccggcatc gcccgtgccg
     3061 agcgtcgacg ccagcgggct gtagatcatc agtcgggatt cggcgtgata agtcggctta
     3121 agccccagaa tgagcggaat gcaagccagc gtcagcaggc cggttatggc ggcgatcatg
     3181 ccgatgcgcc gccgcagcag gcgcaggccc gccgacaggt cgagttcgtc cggcccggag
     3241 tcaaacggtt gcggcggctc atggccgatg agggaacggg taacactgtc ggtggtcggc
     3301 ataagcacgg tcattgggca tgcactcttg tttgtttgcc tgacgaaact gcctcgtaaa
     3361 gtttctccaa ccgccgccca taggcgacca tgctgaacct tgagagatag tgggagcggg
     3421 ccttgctgga gagggcgccg cgcatccacg ggtcgccgac cagtctcgcc aatgccagag
     3481 ccagcgcgtc cttgtcgccg accgggacaa aaagcccggt ctcgccatcg gaaatggctt
     3541 cctcatgcgc gcccacgcgc gtcgtcacca ccgccagccc gtgcgcgagg ccctcgatga
     3601 cggccatcgc catgccttcg gcatgcgaag gcagaaccag aatatcggcg cgcgcgcaca
     3661 gcgcccgcgt tcccttgagg tctatccagc cgggcatcgt aacccggtct gaaaggccca
     3721 tgttcgatgc ctgcctgcga tattcggaga ccggcccgtc gccggcgagc acggcgcgcc
     3781 agggcagatc cgccatgaca gggctgccaa gtgctgccag aagttccggc acgcccttgc
     3841 gctcgcccag ccggccaagg aacaggatga gggggctttc atcgatgcgg accggatgcg
     3901 gaccgggatc gggcacgcaa ttgtgggcga cgattatgcg ggccggatcg acgcccagca
     3961 cttccgacag cgtggcgctg tcgcgccgcc caagcgccac gacccggtcg gcactctgga
     4021 acatggcgcg tatgcgcttt ttctggcgtg gcggcctgac gctgaaatca ttggcatagt
     4081 cataatcgtg cagatgcagg acatggcggc agctgagcgc gcgggcggcg gccgtcagga
     4141 tcagcttgcg cgaggtgctg ccgcgccccg ctatgtggat gtggtggata cgttccggga
     4201 cgaggagccg gtccttcgcc atcagcagga tggccgccga cagacgcatc agcgaggtcg
     4261 gcgcaaacca gcgcggccct ctcgtgtcgg ttaccagatg cgggatgccc gcctcggccg
     4321 aagtgtcggc tatataaccg acaagccggc cgatgccgcc gccattttca cggccgccgg
     4381 caacgtaatg ccggacaggc gcctcgaccc tgcccgacgc gcgcgcgcgt tcggcggcaa
     4441 tggcgacgcc ggcggaaaaa tccgctgcat tcagtttcac gcgcatgact ggaccaccgt
     4501 gcgatagagg caggccgccc gcgcgccgac cgcttcaggg gcgttcgggc cggtcgccca
     4561 ggcaagcgcg ttggcgccaa gcccggtcct gagcgtgacg ctttccgcca atatgcggat
     4621 ggcgcgggca agcatttccg ggtcgcccgg cggaaccacc atgcccagcc cgttaggctc
     4681 gaccgtggcg cgcaattcac cgacatcggt tacgatgacc ggcttgccga aggcggcggc
     4741 caggttgagg acgccgcttt gcgaggcttc cgcatagggc aggacgacca tgtcgcaatc
     4801 gaggaaaagc tgggcgacct ccacatcctc gatgaaccgg ttttgaatat cgtagcgcga
     4861 aggctcgccc atcaacgagc gcagcgtcca tggatcgtca cccctgccgg caatggtgac
     4921 gcgaagattg ggaatggcgt ccttgagcag cgcttcggcg cggatcagct ggtcgagacc
     4981 cttataggcg aagatcctgc cgaacatcag cacgcgcatc gtgccgtcat cggggcggcg
     5041 gaccagcttc tcgcggcggg ccagatcgca atagcgaacg atggaagggt gcgaaagcac
     5101 atgaacgcgg tcgggcgatt ttgaaaagcg gtcgatcgcc atctgcttca gcccgtcgcc
     5161 atgcacgacg acgtggcccg attggcgcac gatcagggtc ggcgcccaat tggggagcac
     5221 gcttgtctcg acatcgccgg gatgaacccg cacgtcatga accgtggtaa ccagcggaat
     5281 cggccgccag aacggcgcgg cgaaattcag ccacagcgtg gtgttgctca gaagatggat
     5341 gaggcttggc cgctcacgcc ggatcagccg cgtcaacttg aacagaaacc acggattgga
     5401 cagggcgcta tgccttggcc aatccatcag ccgcagatcg acggcggggt cgaaccacga
     5461 agccagatgc ccgtagcggc ggcgcggcac gccgagcacc accggcatat ggcgcgcaac
     5521 gccgttgacg aaggaaatgg tgtagtcttc aagatcggcg accaggagca gcgctttcat
     5581 ttcgccgcct ccgtcagtcc gacgcgccgc tcgacgcttg gaagatcgcg caggaggcgc
     5641 agcgcatcgc gcagccggcg cagccgatct ggctcgccgg caaacaaagc
//
LOCUS       DASYCS010000125         2524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_898990,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000125 DASYCS010000000
VERSION     DASYCS010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_898990"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VGM46_04355"
     CDS             <1..430
                     /locus_tag="VGM46_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015314709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY4191856.1"
                     /translation="ADLFIRQQPEGYDTPVGENGVTLSGGQRQRVSIARAIVRRAPIL
                     LLDEATSALDNESEARVQQALTEIMHDTTTIVIAHRLSTVVNADCIIVLEEGRLVEEG
                     THASLIDDRHSVYARFHRLQGENGLGIVDDKATSAAEGTA"
     gene            427..1239
                     /gene="dapB"
                     /locus_tag="VGM46_04360"
     CDS             427..1239
                     /gene="dapB"
                     /locus_tag="VGM46_04360"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006326467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEY4191857.1"
                     /translation="MSEMELVVVGAAGRMGQNLIRTIAAIPGVRLAAAIERAGSSELG
                     KDAGELAGIGQNGIRIGDDPLQAFAKADGVLDFTSPSASIEFAGYAAQARIVHVIGTT
                     GCSAEDDAGIAAAARHATIVKSGNMSLGVNLLAVLVEQAAQALNADDFDIEILEMHHK
                     QKVDAPSGTALLLGEAAATGRAIELAGNDVRVRNGHTGARKAGTIGFATLRGGSVVGE
                     HSVFLSGLGERITLSHHAEDRTIFARGAIKAALWARGRKPGLYSMRDVLGLG"
     gene            1282..1902
                     /locus_tag="VGM46_04365"
     CDS             1282..1902
                     /locus_tag="VGM46_04365"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006326468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEY4191858.1"
                     /translation="MSRTLVLVRHGQSEWNLKNLFTGWRDVDLTEQGHAEAKAAGQKL
                     KARGLKFDIAFTSDLQRAQKTCQHILDAVGQSDLKTIRDQALNERDYGDLSGLNKDDA
                     RKKWGDDQVHVWRRSYDVPPPGGESLKDTGARVWPYYLHDLQPHVLSGGTVLVAAHGN
                     SLRALIMALDGASGQEIVNLELGTGVPIVYTLNADSTVASKEVLAG"
     gene            1994..2078
                     /locus_tag="VGM46_04370"
     tRNA            1994..2078
                     /locus_tag="VGM46_04370"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2028..2030,aa:Leu,seq:cag)
BASE COUNT          470 a          805 c          798 g          451 t
ORIGIN      
        1 cgccgacctg ttcattcgcc agcagccgga aggctacgac acgccggtcg gcgaaaacgg
       61 cgtgacgctt tccggcggcc agcgccagcg cgtttccatc gcccgcgcca tcgtgcgccg
      121 cgcgccgatc ctgctgctgg acgaggcgac atcggcgctc gataacgagt ccgaggcgcg
      181 cgtccagcag gcgctgaccg aaatcatgca tgacaccacc accatcgtca tcgcgcaccg
      241 cctgtccacc gtggtcaacg ccgactgcat catcgttctg gaggaagggc ggctggtcga
      301 agaaggcacg catgcctcgc tgatcgacga ccgccacagc gtttatgcgc gctttcaccg
      361 gctacagggc gagaatggcc ttggcatcgt tgacgacaag gcgaccagcg cggcggaagg
      421 aacagcatga gcgagatgga actggtcgta gtcggggccg ccggccgcat gggccaaaac
      481 ctgatccgca ccattgccgc catacccgga gttcggcttg ccgcggcaat cgaacgggcc
      541 ggctcgtcag agcttggcaa ggatgcgggc gaactcgccg gcatcggtca aaacgggata
      601 aggatcggcg acgatccgtt gcaggccttc gccaaggccg atggcgttct tgatttcact
      661 tcaccctcgg ccagcatcga gtttgccggt tatgccgcgc aggcccgcat cgtccatgtc
      721 attggcacta ccggttgttc ggccgaagac gacgccggga ttgccgccgc cgcccgccac
      781 gccaccatcg tcaagtccgg caatatgagc cttggcgtca atttgctggc cgtgctggtc
      841 gaacaggcgg cgcaggcgtt gaacgccgac gatttcgaca tcgaaattct cgaaatgcac
      901 cacaagcaga aggtcgacgc cccgtccggc acggcgctgc ttctgggtga ggcggctgcc
      961 accggccgcg ccatcgagct tgccggcaat gatgtgcgcg tgcgcaacgg ccataccggc
     1021 gcgcgcaagg cgggaaccat cggctttgcc acgctgcgcg gcggttcggt tgtcggcgag
     1081 cattccgtgt ttctttcggg gcttggcgag cgcatcacgc tgtcgcatca tgccgaggac
     1141 cggacgattt tcgcgcgcgg cgcgataaag gcggccttgt gggcgcgggg ccgcaagccg
     1201 ggcctttatt ccatgcgtga cgtgctggga cttggctgaa cttacctttt ctgaaacaac
     1261 ccgaatgccg aaggagaaga aatgtcgcga actctcgtgc tcgtgcgcca tggccagagc
     1321 gaatggaatt tgaagaacct gttcaccggc tggcgcgatg tcgacctgac cgaacagggc
     1381 catgcggagg caaaggccgc cggccagaag cttaaggctc gcgggctgaa attcgacatc
     1441 gccttcacct cggacttgca gcgggcgcaa aaaacctgcc agcacattct ggacgccgtt
     1501 ggccagagcg atctgaaaac catccgcgac caggcgctga acgagcgcga ctatggcgac
     1561 ctttccggcc tcaacaagga cgacgcccgc aagaaatggg gcgacgatca ggttcatgtc
     1621 tggcgccgtt cctatgacgt gccgccgccg ggcggggaaa gcctgaagga taccggcgcg
     1681 cgcgtatggc cctattacct gcacgatttg cagccgcatg tgctttccgg cggcaccgtg
     1741 ctggtggcgg cgcacggcaa ttcgctgcgc gcgctgatca tggcgctcga cggtgcttcc
     1801 ggccaagaaa tcgtcaatct cgaactcggc accggcgtcc cgatcgtcta cacgctgaac
     1861 gccgattcga ccgtggcatc caaggaagtg ctggctggct aacgtttttt gcgcctgtag
     1921 cggcgcgtgt cggctcaatc gagaaaaagc gcgttgacac tcccggcccg cccgcttaca
     1981 tgagcgcgca ccagcccaga tggcggaatt ggtagacgcg cacggttcag gtccgtgtgc
     2041 cgcaaggcgt ggaggttcga gtcctcttct gggcaccaat ttcaggaaaa gccctgcatc
     2101 ttcaaacaga tgcggggctt tttctttttc gcggccagcc gtggccatcc atccgtccaa
     2161 tccgcgcttc cgcgaaggcg gctgcgtcgc tttccggcgt cggtttccgg tcgaagcctc
     2221 atgctcgatg acgccatgcc gccgctggcc ggtaagcgca agccggccat gaaatggctg
     2281 cctgcctgcg gcgccggttg tgtgggccgg tcttgcgccg gcgccccaac aacgcaataa
     2341 cgccggcgtc accggtcccg aaagcgctat ggcaaacgat aaaatctcat ccctgcagtc
     2401 cgtggctagc gtcggggagt gcatgatcga actgtcggcc agcgcatcgg gttggcagat
     2461 gggccatgcc ggcgatgcct tcaacaccct atggaacctg agggccttgc tgccggaaac
     2521 cgtc
//
LOCUS       DASYCS010000126         2365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1185883,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000126 DASYCS010000000
VERSION     DASYCS010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185883"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="VGM46_04375"
     CDS             complement(<1..599)
                     /locus_tag="VGM46_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HEY4191859.1"
                     /translation="MHFRSILSGTLAVLLLSGTAAFAAGSANPSEGGFSFHLPSLTLP
                     GLLGGKKEAPTQLAQTDGAGVTGLEEQIRQMNGKIEELNFQVLQMQEQIRKQQEDNEF
                     RFQQLEQGAPAGGQKKSDAGATAPAAGAASVAQAPATATPPGGGESVEDVIVDSGTGD
                     PGTLIPGSGNDGATGAPPKNFGTITVDKNGNVVDANGNT"
     gene            complement(806..1312)
                     /gene="pal"
                     /locus_tag="VGM46_04380"
     CDS             complement(806..1312)
                     /gene="pal"
                     /locus_tag="VGM46_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEY4191860.1"
                     /translation="MRRIARLAANPAMIALFASLAIAGCASKKNLNSASDLGLNGAGA
                     ATPGSSQDFTVNIGDRIFFDTDSTSIRADAQQTLARQAQWLNKYSQYSIVVEGHADER
                     GTREYNIALGARRAAATRDFLAAKGVSSGRMKTISYGKERPVAVCDDISCWSQNRRAV
                     TTIGGAGS"
     gene            complement(1546..>2365)
                     /gene="tolB"
                     /locus_tag="VGM46_04385"
     CDS             complement(1546..>2365)
                     /gene="tolB"
                     /locus_tag="VGM46_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006327721.1"
                     /note="forms dimers; may be involved in cell envelope
                     integrity; interacts with outer membrane proteins and with
                     the C-terminal domain of inner membrane protein TolA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HEY4191861.1"
                     /translation="GYFDTRIVFIDESGAKNARQKRLAIMDQDGANVRYLSNGKSIVL
                     TPRFSPNRQEITYMSYESGQPRVYLLQIETGQRELVGNFPGMTFAPRFSPDGQKVIMS
                     LLRDDGNSNIFAMDLRSRATTRLTNSTAIDTSPSYSPDGSRVVFTSDRGGRAQIYVMG
                     ADGSGQTRISFGDGTYSTPVWSPRGDLIAFTKQTAGEFQIGVMRTDGSGERILSSGFQ
                     QEGPTWAPNGRVLMFFRDSAGGPKLVSVDLTGRNEQSIPTANFASDPAWSPLLE"
BASE COUNT          431 a          689 c          773 g          472 t
ORIGIN      
        1 tgcgtgttgc cgtttgcatc cacgacattg ccattcttgt cgacggtgat ggtgccaaaa
       61 ttcttcggtg gcgcgccggt tgcgccatcg ttgccgctgc ccggtatcaa cgtgccggga
      121 tcgcccgtcc cggaatcgac gatgacgtcc tcgacgcttt cgccgccgcc cggcggcgtt
      181 gccgtcgccg gggcctgcgc gacgctggcg gcgccggctg cgggcgcagt cgcccccgca
      241 tccgattttt tctgcccgcc ggcaggcgcg ccctgttcaa gctgctggaa acggaactcg
      301 ttgtcttcct gctgcttgcg gatctgctcc tgcatttgca ggacctggaa gttcaactcc
      361 tcgatcttgc cgttcatctg gcggatctgt tcttccaggc cggtgacgcc ggcgccgtcg
      421 gtctgggcca gctgcgtcgg ggcttccttt tttccaccga gcaggccggg caaagtgagg
      481 gaaggcaggt ggaaggaaaa gccgccttcg gacgggttcg cggagccggc ggcaaaagcc
      541 gccgtgcccg acaatagcag caccgcaagc gtgccgctca ggatagatct gaaatgcata
      601 tgcctctcgc cattgaaaca aggaatgttc atgccgaacc cgaccgctgt gcttgatatg
      661 cctatcgata cacgcgaagt cgcctccggc accgttgcct tctagcagga cggcagactt
      721 cggccaaatt ttgtttgagc cgtgcaaacg cgaaaaaggc ggcctttcgg ccgcctttca
      781 cgaaaaactg ttgcataaat gccgatcagc tacccgcgcc accgatggtg gtgacggcgc
      841 ggcgattctg cgaccagcag gaaatgtcgt cgcagacggc gaccgggcgt tccttgccgt
      901 aggagatcgt cttcatgcgg ccgctggaga cgcctttggc ggccaggaag tcgcgggtgg
      961 cggcggcgcg gcgggcgccg agcgcgatgt tgtattcacg cgtgccgcgc tcgtcggcat
     1021 ggccttcgac gacgatgctg tattggctgt atttgttgag ccactgtgcc tggcgggcca
     1081 gcgtctgctg ggcgtcggcg cggatcgagg tggaatccgt atcgaagaag atgcggtcgc
     1141 caatgttgac ggtgaagtcc tgcgacgaac cgggggtagc ggcgccggct ccgttgaggc
     1201 caagatcgga ggcgctgttg aggtttttct tcgaggcgca accggcaatc gccaacgagg
     1261 cgaaaagcgc gatcatggcc gggtttgctg caagtcttgc gatacggcgc atgccgcctc
     1321 tccttcgcat ttcgagtgaa ttcattgatc agccagtaac cacgtttggg ttaaccgccg
     1381 ctcaacaaac aaggttaata tttggtttca gccgccgtcc gcgaccgttt tgccgatgcg
     1441 atattccatc gggaccgtag gcttaaatgc ggccaaaacg cgacaatgac agtaaaagag
     1501 gcccaaaagg gcctctttta tcgactgttt caaaaaagcc ggcttctatt ccagcaaggg
     1561 cgaccacgct ggatcggaag cgaagttggc tgtcggaatc gactgttcgt tgcggccggt
     1621 aaggtcgacg gaaacgagtt tcggtccgcc ggcgctgtcg cggaagaaca tcagcacgcg
     1681 gccattgggc gcccaggtcg gcccctcctg ctggaaaccg gacgacaaga tgcgctcgcc
     1741 ggaaccatcg gtgcgcatga cgccgatctg gaattcgccc gccgtctgct tggtaaaggc
     1801 gatgaggtcg ccgcgcggcg accataccgg cgtcgaatag gtgccgtcgc caaacgaaat
     1861 gcgggtctgg cccgagccgt ccgcgcccat gacgtagatc tgcgcgcgcc cgccacggtc
     1921 ggaggtgaag acgacccggg agccgtcggg cgaataggag ggcgaggtgt cgatggcggt
     1981 ggagttggtc agccgcgtgg tcgcgcggct tcgcaaatcc atggcgaaga tgttggaatt
     2041 gccgtcgtcg cgcagcaggc tcatgatgac cttctggcca tcgggcgaaa agcgcggcgc
     2101 aaaggtcatg ccggggaaat tgccgaccag ttcgcgctgg ccagtctcga tctggagtag
     2161 ataaacgcgg ggctggccgc tttcatacga catataggtg atttcctgcc ggttcggcga
     2221 gaaacgcggg gtcaagacga tggacttgcc gttggacaga tagcgcacat tggcgccgtc
     2281 ctggtccatg atggccagcc gcttctggcg ggcgttcttg gctccggact cgtcgatgaa
     2341 gacgatgcgg gtgtcgaaat agccc
//
LOCUS       DASYCS010000127         1844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4378582,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000127 DASYCS010000000
VERSION     DASYCS010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4378582"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(458..1372)
                     /locus_tag="VGM46_04390"
     CDS             complement(458..1372)
                     /locus_tag="VGM46_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015314146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY4191862.1"
                     /translation="MSLAHRPAIKNLLLAGILLMLAGDFMFALNDAMGKWLVASFSVG
                     QVVLIRSIGAFIVLGPLLARQGTAKLFQMERRDLQFLRVVATTMDTSFFYAAVVFLPL
                     ADVMSFYMAGPIYVAALSHFLLGERVGWRRWTAILLGFCGVLIMLKPSSAAFSTSSIF
                     ALIGSLSFAFSIVLNRRLRGTSDTSLVTWQTIGTLVVGGALTIGAWRAPSAFDVGAML
                     LLGIVSCIAHLLITRALKLAPASTLAPLHYTLLLWAIVFGLVFFGDMPSLRILIGAGV
                     VVLAGLFIFHRKKVVDEIVPADVPKGVS"
BASE COUNT          389 a          549 c          598 g          308 t
ORIGIN      
        1 agcggcctgc cgccgcggct gcgttccttg ccggcgcgga tcatcaacgc cgctgttgtc
       61 tgccggtcgg cgatactgac cggcataccg ccgaccacca ccgttggaac ttcggccagg
      121 acttcttcaa acgactgcaa cgaggcctcc tgccgccccc acggcgcaag tggggatcat
      181 tccgtagcat tgccaagcgg catgcagccc gatgcgatcg aagcggacaa ggccaattca
      241 cctttccacc cgaactacac ctgagtcgct aactgatgct atccatccgt catttcctac
      301 gatgaaacgt ccggccagtt aagctcattg ttaaagaata gttaactgta acaagtttaa
      361 tcaatattta aacatcggtt tttcttgggc ggacagcgcc gttattgttt ccgcaggcgg
      421 gccggatttt gccttgagga agtaagacga ccgggcgtca gctgacgccc ttgggcacgt
      481 ccgccggaac gatctcatcc accactttct tgcggtgaaa gatgaacagg ccagccagaa
      541 cgacgacgcc ggcgccaatc aggatacgca ggctcggcat atcgccgaag aaaaccaggc
      601 cgaagacgat ggcccagagc agcagcgtgt aatgcagcgg cgccagcgtc gaggccgggg
      661 caagcttcag ggcgcgtgtg ataagcagat gcgcgatgca ggatacgatg ccgagcagca
      721 gcatggcccc gacatcgaag gcgcttggcg cgcgccaggc gccgatggtc agcgcgccgc
      781 cgaccaccaa cgtgccgatg gtctgccagg tgaccagcga ggtgtcgctg gtgccgcgaa
      841 gccggcggtt gagaacgatg gagaaggcaa aggacaggct gccgatcagg gcgaagatcg
      901 acgacgtgga aaaggcggcc gaggatggct tgagcatgat cagcacgccg caaaagccaa
      961 gcaggatggc ggtccagcgc cgccagccca cccgttcgcc aagcaggaag tgcgagaggg
     1021 cggccacata gatcggcccg gccatgtaga agctcatgac gtcggcaagc ggcaggaaaa
     1081 ccaccgccgc ataaaagaac gaagtgtcca tcgtggtggc gacgacgcgc aggaactgga
     1141 gatcgcgccg ttccatctgg aacagttttg ccgttccctg cctggccagc agcggcccca
     1201 gcacgatgaa tgcgccgatg gaacggatga gcaccacctg gcctacggaa aaggacgcaa
     1261 ccagccattt gcccatggcg tcgttcaggg cgaacatgaa gtcgccggcc agcatcagca
     1321 ggatgccggc caggagcagg ttcttgatcg caggtctgtg ggcgagactc atgccggcat
     1381 tccttgtggc cgaagggccg gtaatgcggc tgttagcccg gcttgatgcg ctttacgagg
     1441 aaaatccgtt ggcgcaggca acccaacgga ttttgcctcg atccggcctg accggcaagg
     1501 cgtgcgcggt tggacgacgg cccgacgatg gatgacgcac ggcgtgggca gcggggcaaa
     1561 attgcggcct tggtgttgcg cggcgcgccg gtcgaggccg atgcgctgga gactgccggc
     1621 gcaagcatct tttctgctgg agaccgggcg agcagcactt ggcttgaccg gtagcccgtt
     1681 ggttccgcgc cgttatccat tcgctcaaaa gcacagccac cgcgacacga ataatggcgc
     1741 ggcgtggcgg tagctcgcgg ggtatgtcgg gaggcgacag gcggcccccg agttttgccg
     1801 ctattgcatc ttgtggcggc ggttgatcga gccgggccgc ccgc
//
LOCUS       DASYCS010000128         7746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3653323,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000128 DASYCS010000000
VERSION     DASYCS010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 7746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7746
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3653323"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..901)
                     /locus_tag="VGM46_04395"
     CDS             complement(89..901)
                     /locus_tag="VGM46_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018429784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HEY4191863.1"
                     /translation="MAGFHVMADAHGALMQPNVRRITTGDIFDALRLGWRDFAAKPSH
                     YVFLCLIYPIAGVVLTRWSSGGNAIQLIYPLMAGFALLGPLAALGLYEISRRRELGLD
                     AGWRHALGVRRSSALPSIMVLGIMLLALFLLWLFTAQSIYTSLFGDLPPSSVTAFLRE
                     VLTTDRGWTLIIAGNAVGFVFAVIALATTVVAFPLLLDRDVGAVAAIETSARAVMVNP
                     VQMALWGIMVAFLLGLGSLPLFAGLAVVMPVLGHATWHLYRKLVEPERPRPM"
     gene            1097..1627
                     /locus_tag="VGM46_04400"
     CDS             1097..1627
                     /locus_tag="VGM46_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010915459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEY4191864.1"
                     /translation="MERIPGTSRSLTGPVTHETDRRTVLVGGGLALLAGLAGCGSLPQ
                     GNAPAAGASASASDILAGIRHANGLGSLSSDARLEQAARQQAGYMAGAGRMSHTTGWG
                     KDFAARVKDNGIEGAAAENIAEGRMDLPRLFDMWMRSPPHRRNMLDARFGHFGLAYAG
                     GGKNGQWRYWALVLGR"
     gene            complement(1686..3224)
                     /locus_tag="VGM46_04405"
     CDS             complement(1686..3224)
                     /locus_tag="VGM46_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEY4191865.1"
                     /translation="MAQAGGIDPNNVPHRGLITGSLMLATIMQALDTTIANVALPTMT
                     GNLGASPDNITWVLTSYIVAAAIMTPMTGWLSDRFGRKELFLTSVVGFTVASMLCGLA
                     WSLESIVLFRLLQGIFGAAIVPLSQTFLLDINPKERHGQAMAIWGAGIMLGPILGPML
                     GGWITENLTWRWVFFIKLPIGIVAFLGMAAYLPKIAARARSFDFFGFAMLSLGVGALQ
                     LMLDRGGEVDWFSSVEIWTELALSITGFWVFTIHTLTAEQPFIDPRIFLDRNFVTGLL
                     VIFVMGVLILASMALLPPMLSTIFGYSTVTTGLVMGPRGVGTMASMLIAGRIMHKIDP
                     RLLVVVGFLLTAQSLYTMVGFSPQMDDRLILTSGVIQGLGMGMVFVPLSTIAFATLDA
                     RFRTDATALFSLVRNIGSSIGVSVMTVMLTRNIQINHTELSVNINPFNPNLWVASPTA
                     ATGDPTALSQVDGMVNIQSQMISYVNDFKLLMIMTLCAIPFVLLLKKPKISAAGGAPA
                     VHMD"
     gene            complement(3249..4418)
                     /locus_tag="VGM46_04410"
     CDS             complement(3249..4418)
                     /locus_tag="VGM46_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018427185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEY4191866.1"
                     /translation="MNAVAKIDENVSKLDMESPAQPATPVSVAIPDNPEPAPRKKTRR
                     RGRFLLMLAVPLALIAAGAYVWVTGGRYQETENANLRQARVAIASDTAGRIVTVNVAD
                     NQTVKKGDVLFAIDPEPYRIALAQADAAVATARLNVEQLRAAYSQSMAQERSAASEVD
                     YAQSQFSRASDLATKGFNAKSALDEARNDLAKAKQQLAVDKQGIESAKAALGGNPDIE
                     ADKHPSVMAALASRDKAAYDLELTSVRAPADGVAYQAASFKVGQFVGAGMPLFSLVET
                     GDTWIDANFKETQLTQMKPGQKAEVVLDTYPDKKFEATVQAIGAGTGAEFSLLPAQNA
                     TGNWVKVTQRIPVRLEFTDPDAKMALRSGMSADVSVDTGASRGWGSIFGKASAAE"
     gene            complement(4415..4897)
                     /locus_tag="VGM46_04415"
     CDS             complement(4415..4897)
                     /locus_tag="VGM46_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008875095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY4191867.1"
                     /translation="MVIDGLDRLGFLIHDAARLMRRRFESRASGLGLSAAQWRLLVRV
                     TKEEGVAQARLAELLEIEPISVSRLIDRMEEGGWIERRSDATDRRVRTIFPTERSREV
                     YAGVKGLAGAVYEQALTGLSLDERRILVKGLFTVIDNLSDGETMSCPPTKKTAESAAT
                     "
     gene            5203..5499
                     /gene="groES"
                     /locus_tag="VGM46_04420"
     CDS             5203..5499
                     /gene="groES"
                     /locus_tag="VGM46_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015338933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEY4191868.1"
                     /translation="MAKSKFRPLHDRVVVRRVESEAKTAGGIIIPDTAKEKPQEGEIV
                     AVGSGARDDSGKIVALDVKAGDRILFGKWSGTEVKLNGEDLLIMKESDIMGIIG"
     gene            5577..7229
                     /gene="groL"
                     /locus_tag="VGM46_04425"
     CDS             5577..7229
                     /gene="groL"
                     /locus_tag="VGM46_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006643166.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEY4191869.1"
                     /translation="MAAKEVKFSRDARERMLRGVNILADAVKVTLGPKGRNVVIDKSF
                     GAPRITKDGVTVAKEIELEDKFENMGAQMVREVASKTNDKAGDGTTTATVLAQAIVQE
                     GHKYVAAGINPMDLKRGIDMAVADVVAQLVKSAKKIKTSEEVAQVGTISANGDESVGK
                     MIAEAMQKVGNEGVITVEEAKTAETELEVVEGMQFDRGYLSPYFVTNPDKMIADLEDV
                     YILLHEKKLSNLQAMLPILEAVVQSSKPLLIISEDVEGEALATLVVNKLRGGLKIAAV
                     KAPGFGDRRKAMLEDIAILTGGQVISEDLGIKLENVSLAMLGRAKKVSISKENTTIVD
                     GAGKKAEIQGRVAQIKQQIEETTSDYDKEKLQERLAKLAGGVAVIRVGGSTEVEVKEK
                     KDRVDDALNATRAAVEEGIVAGGGVALLRASTNIKATGANADQTAGISIVRKALQAPC
                     RQIASNAGEEASIVVGKILDNKGVSFGYNAAKDQYGDLIALGIVDPVKVVRTALQDAA
                     SVAGLLVTTEAMIADAPKKDNGGGGMPGGMGGGMGGMGGMDF"
     gene            7417..7596
                     /locus_tag="VGM46_04430"
     CDS             7417..7596
                     /locus_tag="VGM46_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEY4191870.1"
                     /translation="MVNKDQVAGAAKQAKGSIKDAVGKATGSKKTQAEGKADKAAGKV
                     QKTYGDAKEKIKKAL"
BASE COUNT         1617 a         2393 c         2397 g         1339 t
ORIGIN      
        1 attgtccaac ttgcgtatcg tcagctaaga cacaaggtgc ccggaacgca gccgttccgg
       61 gcattggctt tcgccttgcc taccggcctc acatcggccg cggccgttcc ggctcgacaa
      121 gcttgcggta caggtgccag gtggcgtggc cgagcaccgg catgacaacc gccagccccg
      181 cgaaaagcgg cagcgagccg aggcccagca gaaacgccac cattatcccc cacaaggcca
      241 tctgcaccgg attgaccatc accgcgcggg ctgaggtttc gattgcagct accgcgccga
      301 catcccggtc gagcagcagc ggaaaggcga ccacggtggt ggccagcgcg atcaccgcaa
      361 aaacaaagcc gacggcattg ccggcgataa tcagcgtcca accccggtca gtcgtcagca
      421 cttcgcgcag gaatgcagtg accgaagatg gcggtaaatc gccgaacagg ctggtataaa
      481 tcgactgcgc cgtgaacagc cacagcagga acagcgccag aagcatgatg ccgagcacca
      541 tgatggacgg cagcgccgac gaacggcgca ctccaagcgc gtgtcgccag ccggcatcca
      601 gcccgagttc gcgccggcgg ctgatttcat agaggccgag tgccgcaagc ggcccgagca
      661 gggcgaaacc ggccatcagg ggatagatga gttggatggc gttgccgccg cttgaccagc
      721 gcgtcagcac cacgccggcg atcgggtaga tcaggcacag gaagacgtag tgggagggct
      781 tcgcggcgaa gtcccgccac ccaagacgca acgcatcaaa aatatcgcct gtggtgatgc
      841 ggcgcacgtt gggctgcata agggcaccgt gcgcatctgc catgacatga aagccggcca
      901 taaccttcct cccgtgtgga gggcggtcac cgccggggtg gccacccgtg gctgccactt
      961 caataagcag cgcaacctta gccgatccgg gccgtcttgt cgttgatttg ttccagcgct
     1021 cgcgaaagct tgtattccag cccgcttttt tccctttgcc cgccaaaagc catgatcggc
     1081 gctaaatcag gcacctatgg aacgcattcc cggcacttcc cggtccttga ccggcccagt
     1141 aacgcatgaa acggatcggc ggaccgttct ggtcggcggt ggcctggcat tattggcagg
     1201 ccttgccgga tgcggctccc tgccccaagg caatgctccc gccgccggcg catccgcttc
     1261 cgccagcgac attctggcgg gcatacgcca cgcgaacggt ctcggttcat tgtcgtccga
     1321 cgcaaggctt gaacaggcgg cacggcagca ggcgggctat atggccggcg ccggccgcat
     1381 gagccacacg accgggtggg gcaaggattt tgccgcccgc gtcaaggaca acggcatcga
     1441 aggagcggcg gccgaaaaca tcgcggaagg ccggatggat ttgccgcgcc tgttcgatat
     1501 gtggatgcgc tcgccgccgc atcgccgcaa catgctcgac gcgcgcttcg gccatttcgg
     1561 cctcgcctat gccggcggcg gcaagaacgg ccagtggcgc tactgggcgt tggtgctggg
     1621 caggtaagca ggcgcggaac ccgcccttca cgctcacgcg caaagggctt tcgagcgccc
     1681 gccgcttaat ccatatgaac ggccggcgcg ccgcctgcgg ccgatatctt cggctttttc
     1741 agcagaagaa caaatgggat ggcgcataac gtcatgatca tcagcagctt gaaatcgttg
     1801 acataagaga tcatctgcga ctggatgttg accatgccgt cgacctgcga caacgccgtc
     1861 ggatcgccgg tggccgctgt cggcgaggcc acccaaaggt tgggattgaa cggattgatg
     1921 ttcaccgaca gctcggtgtg gttgatctgg atattgcggg tcagcatcac cgtcattacc
     1981 gaaacgccga tcgacgagcc gatgttgcgc accaggctga acagcgccgt ggcatcggtg
     2041 cggaagcgcg cgtccagcgt ggcaaatgca atggtcgaga gcggcacgaa caccataccc
     2101 atccccagac cctggataac gcctgaggtc aggatcagcc ggtcgtccat ctgcggcgag
     2161 aagccgacca tcgtatagag cgattgcgcg gtcagcagaa atccaacgac gacgagcagc
     2221 ctcgggtcga tcttgtgcat gatccgcccg gctatcagca tcgaggccat cgtgccgacg
     2281 ccgcgcggcc ccatgacaag gccggtggtg acggtcgaat agccgaagat cgtcgacaac
     2341 atcggcggca gcagcgccat gctcgccaaa atgagcacgc ccatgacgaa gatcaccagc
     2401 aggccggtaa cgaaattgcg gtccaggaaa atcctcggat cgatgaaggg ttgctccgcc
     2461 gtcagcgtat ggatggtgaa cacccagaag ccggtgatgg acagggcaag ttcggtccag
     2521 atctcgacgg acgagaacca gtcgacctcg ccgccccgat cgagcatcag ctgaagcgcg
     2581 ccgacgccga gcgacagcat cgcaaagccg aagaagtcga agctgcgggc gcgcgccgcg
     2641 atcttgggca ggtaggcggc catgccgagg aaggcgacga tgccgatcgg cagcttgatg
     2701 aagaacaccc agcgccatgt gaggttttcg gtgatccagc caccgagcat cgggccgagg
     2761 atcggcccta gcatgattcc ggcgccccag atggccatgg cctggccgtg gcgctcctta
     2821 gggttgatgt cgagcaggaa tgtctgcgac agcgggacga tggcggcgcc gaagatgcct
     2881 tgcagcagcc ggaacagcac gatgctttcc aggctccacg ccaggccgca cagcatagag
     2941 gcgacggtga acccgaccac ggaggtgaga aacagctcct tgcgcccaaa ccggtcggac
     3001 agccagccgg tcatcggcgt catgatcgcc gccgcgacga tataggacgt cagaacccag
     3061 gtgatgttat ccggcgaggc gccgagattg ccggtcatgg tcggcagggc gacgttggcg
     3121 atcgtggtgt cgagcgcctg catgatcgtc gccagcatca acgaaccagt aatcagcccg
     3181 cgatgcggga cattgttcgg gtcgatcccg cctgcctgtg ccatgatatc tactttacgg
     3241 ttgtattttt attctgctgc gctggccttg ccgaaaatgc tgccccagcc gcggctggcg
     3301 ccggtgtcga ccgacacgtc ggcgctcatg cccgagcgca gcgccatctt ggcatccggg
     3361 tcggtaaatt cgaggcgcac gggaatgcgc tgcgtgacct tgacccagtt gccggtggcg
     3421 ttctgggccg gcagcaacga gaattccgcg ccggtgccgg cgccgatggc ctggacggtc
     3481 gcctcgaatt tcttgtccgg ataggtgtcg agcacgactt cggctttctg gccgggcttc
     3541 atctgggtga gctgcgtttc cttgaaattg gcgtcgatcc aggtatcgcc ggtctcgacc
     3601 agcgaaaaca gcggcatgcc ggcgccgacg aactgcccga ccttgaacga tgccgcctga
     3661 taggcgacgc catcggccgg cgcgcgcacc gaggtcagtt ccagatcgta ggcggccttg
     3721 tcgcgcgatg ccagcgcggc catgacgctc ggatgcttgt cggcctcaat gtccggatta
     3781 ccgcccagtg ccgccttggc actctctatg ccctgcttgt ccacggccag ttgctgcttg
     3841 gccttggcaa ggtcgttgcg tgcttcgtcg agcgcggatt ttgcgttgaa gcccttggtg
     3901 gccagatcgg aggcgcggct gaattgcgat tgtgcgtagt cgacctcgct ggcggccgag
     3961 cgctcctgtg ccatggactg gctgtaggcc gcccgcaatt gttcgacatt gaggcgcgcg
     4021 gtggcgaccg ccgcatcggc ctgggccagc gcgatccggt aaggctcggg gtcgatggca
     4081 aacagcacat cgcccttctt gacggtctgg ttgtcggcga cattcactgt gacgatacgg
     4141 ccagctgtgt ccgaggcgat ggcgacgcgc gcctgacgca ggtttgcgtt ttccgtctcc
     4201 tgatagcggc cgccggtgac ccagacatag gcgccggccg cgatcaacgc gagcgggaca
     4261 gccagcatca gcaagaaacg cccgcggcgg cgggtcttct tcctgggggc aggctcggga
     4321 ttgtcgggaa tggcgacgga aacaggcgtg gcgggctgcg ccggcgattc catgtcaagc
     4381 ttgcttacgt tttcgtctat tttcgccacg gcgttcatgt tgctgcgctc tctgcggttt
     4441 tcttggtagg cgggcaggac atcgtctctc catccgagag attgtcgatg accgtaaaaa
     4501 gccctttgac aaggatgcgg cgttcgtcga gcgacaagcc cgtcagcgcc tgttcgtaca
     4561 cggccccggc cagccctttt acgccggcat agacctcacg agacctctcg gtcggaaata
     4621 tggtccgcac gcgcctgtcg gttgcatcgg aacgacgttc gatccaaccg ccctcctcca
     4681 tccggtcgat caagcgcgaa acgctgatcg gctcgatttc cagaagttcg gcaagcctgg
     4741 cctgcgcaac gccttcctct ttcgtaacgc gaacgagcaa ccgccattgt gcagccgaaa
     4801 gcccaagacc actggcgcgc gattcaaagc gccggcgcat caaccgcgcg gcgtcgtgaa
     4861 tgagaaagcc aagtctgtcc aagccgtcga taaccatgag cggcatatat gataagcgtg
     4921 cttatcaatc aagataccgc aacgcaccaa accatctaaa aattgcaatg cagccatgcg
     4981 ccatatggtt ggcgtaaatt tccggtaaat ttgaaactgg catgtcacac gacggaggcg
     5041 gtttaagtgc tagcacctca tttgcgggcc atcgtccttg actcggcacg gccgccggtt
     5101 tatctcaccg gcacgttagc actcacctta catgagtgct aacccagtcc gggccatgcc
     5161 tggacggagg aaatggacaa caatcccact gaggacgtaa aaatggcaaa gtcgaaattc
     5221 cggccgctgc atgaccgcgt ggtcgttcgc cgcgtcgaat ccgaggcaaa gacggccggc
     5281 ggcatcatta tccccgatac ggccaaggaa aagccgcagg aaggcgaaat cgtcgccgtc
     5341 ggctccggcg ctcgcgacga tagcggcaag atcgttgccc tggacgtcaa ggccggcgac
     5401 cgcatcctgt tcggcaagtg gtcgggcacc gaagtcaagc tcaatggcga agaccttctg
     5461 atcatgaagg aatccgacat tatgggcatc atcggctaat caagccgcga tcacccgcct
     5521 atccatccca atttcgggca atctgaattt cgggcgcaat gcccaggagc acatcaatgg
     5581 ctgccaaaga agtaaaattc tcccgtgacg cccgcgagcg tatgctgcgc ggcgtcaaca
     5641 tcctcgccga tgcggtgaag gtgacgcttg gtcccaaggg ccgcaacgtc gtcatcgaca
     5701 agtcgttcgg cgccccgcgc atcaccaagg acggcgtcac cgtcgccaag gaaatcgagc
     5761 ttgaagacaa gttcgagaac atgggcgcgc agatggtccg cgaagttgct tcgaagacca
     5821 acgacaaggc cggcgacggc actacgactg cgaccgttct ggcccaggcc atcgtccagg
     5881 aaggccacaa atacgttgcc gccggcatca acccgatgga cctgaagcgt ggcatcgaca
     5941 tggctgtcgc cgatgtcgtg gcccagctcg tcaagtccgc caagaagatc aagacctcgg
     6001 aagaagtcgc tcaggtcggc acgatctcgg ccaatggcga cgaatctgtc ggcaagatga
     6061 tcgcggaagc gatgcagaag gtcggcaacg agggcgtcat caccgtcgag gaagccaaga
     6121 ccgccgagac cgagcttgaa gtcgtcgaag gcatgcagtt cgaccgcggc tacctgtcgc
     6181 cctacttcgt caccaacccc gacaagatga ttgctgatct cgaggacgtc tacattctcc
     6241 tccacgagaa gaagctttcg aacttgcagg caatgctgcc gatcctcgaa gctgtcgtgc
     6301 agtcgtccaa gccgctgctc atcatctcgg aagacgtcga aggcgaggcc ctggccacgc
     6361 tggtcgtcaa caagctgcgt ggcggcctga agattgctgc cgtcaaggca ccgggcttcg
     6421 gcgatcgccg caaggctatg ctggaagata tcgccatcct caccggcggc caggtcattt
     6481 ccgaagacct cggcatcaag cttgagaacg tcagccttgc catgctcggc cgcgccaaga
     6541 aggtgtcgat ctccaaggag aacaccacca tcgtcgacgg cgccggcaag aaggccgaga
     6601 tccagggccg cgttgcccag atcaagcagc agatcgagga aaccacctcg gactacgaca
     6661 aggagaagtt gcaggaacgt ctggcgaagc tcgccggcgg cgttgcggtc atccgcgtcg
     6721 gcggttcgac ggaagtcgaa gtgaaggaaa agaaggatcg cgtcgatgac gcgctgaacg
     6781 caacccgcgc tgccgtcgaa gaaggcatcg tggctggcgg cggcgtcgcc cttttgcgcg
     6841 cctccaccaa catcaaggct accggcgcga atgcagacca gaccgccggc atctccatcg
     6901 ttcgcaaggc actccaggct ccgtgccgcc agatcgcctc gaacgccggt gaagaagcct
     6961 cgatcgtcgt tggcaagatc ctcgacaaca agggcgtttc cttcggctac aacgcagcca
     7021 aggaccagta tggcgacctg atcgctctcg gtatcgtcga cccggtcaag gtcgtgcgca
     7081 ccgcgcttca ggacgcagcc tcggttgccg gcctgctggt aaccaccgaa gccatgatcg
     7141 ccgatgctcc gaagaaggac aatggcggcg gcggcatgcc tggcggcatg ggcggcggca
     7201 tgggcggcat gggtggcatg gacttctaag tccacaccca cgcaccttcg catctacgca
     7261 aagggcgccc tcgggcgccc tttttgtttg gctggaagaa agccgtcaat ccggccttcg
     7321 gcctcgaaaa acaaagcgct ggccaagctg gaacctcgcc ccgcctcgtc cgttttccca
     7381 ggcaagttgc agcacaacaa aaccgggaga tgcacgatgg tgaacaagga tcaggtggct
     7441 ggcgcggcca agcaggcaaa gggttcgatc aaggatgcgg tcggcaaggc gaccggcagc
     7501 aagaaaacgc aggccgaagg caaggccgac aaggctgccg gcaaagtgca gaagacctat
     7561 ggcgacgcca aggagaagat caagaaagcg ctttgatccc aatcgacgct tggcgtgaat
     7621 gcaaggcggc tttggccgcc ttttttcgct tcattgccga gcagctgccg gattgtgcga
     7681 gaatcgatga cagcccatcg ccgggcgcac gggaatatga attttcgtac agccggcaaa
     7741 ccggcc
//
LOCUS       DASYCS010000129         3505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1186840,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000129 DASYCS010000000
VERSION     DASYCS010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1186840"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..811)
                     /locus_tag="VGM46_04435"
     CDS             complement(149..811)
                     /locus_tag="VGM46_04435"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEY4191871.1"
                     /translation="MAMLTASGCTHSVDMSALQLDNMSTGSIRPIRPSISVDKHITSP
                     DVMYAGLTDGGFGVPAVPYQKVKPEFRRQIVVDPTGERPGTIVVRLQERHLYLVQEGG
                     EAIRYGVGIGKDGFSWSGRANVQYGREWPTWTPPPEMIQRKPELVKWQGGQPGGLQNP
                     LGARALYLFQDAKDTGYRIHGSPEWWSIGQAMSSGCVRLINQDIIDLYSRVSKKNPVL
                     VV"
     gene            1104..1727
                     /locus_tag="VGM46_04440"
     CDS             1104..1727
                     /locus_tag="VGM46_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEY4191872.1"
                     /translation="MTIEIWAAFCAASMILLIIPGPTILLVVSYALGQGWRIALPMAA
                     GVALGDFTAMTLSMLGIGALLAASATVFTILKLIGAAYLVYLGIKLFRAGGALRAEPR
                     TDARSAARMMAHACLVTALNPKSITFFVAFLPQFLDRNGDFWMQMLIFEATFLTLAFA
                     NAFGYALVAARARTLVGNQKAIRVFNRTGGTLLVGAGIATVAIRSGG"
     gene            1893..3347
                     /gene="xylB"
                     /locus_tag="VGM46_04445"
     CDS             1893..3347
                     /gene="xylB"
                     /locus_tag="VGM46_04445"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HEY4191873.1"
                     /translation="MYLGLDLGTSGVKALLIDASQKIIGAGHAALDVARPHPGWSEQD
                     PAQWIRACEEAIGELKATHGKELAAVKGIGLSGQMHGATLLDRDDKVLRPSILWNDTR
                     SHAEAAALDADPRFRAITGNIVFPGFTAPKLAWVRNNEAGLFGKVAKVLLPKDFLRLW
                     LTGEHISEMSDAAGTSWLDTGKRRWSPELLAATFLDEKHMPTLVEGTEKAGALRGELA
                     AKWGMAAGIPIAGGAGDNAASACGMGTVGEGHAFVSLGTSGVLFAANGCYLPNPASAV
                     HTFCHALPNTWHQMGVILSATDSLNWLSEITGKDARALTDELGDTLKAPTGVSFLPYL
                     SGERTPHNDSAIRGSFTGLAHESGRPALTQAVVEGVAFAFRDSLEALAKAGTTLTRVT
                     AIGGGSRSRYWLKAIATALGIPVDIPADGDFGAAFGAARLGLITATGADPLSICSAPA
                     TEATIEPDGELRDAYAQAYGRYRALYPAIKGATA"
BASE COUNT          600 a         1143 c         1098 g          664 t
ORIGIN      
        1 gaccatggtc tggttgatga cggcggtggg gccgccgccc tgcgcgatca cgaaggttcc
       61 ggacattctc gctcctccag aggtcaattg acaggcgttg gcctgtcttt ggcatccggt
      121 ggcggaggag gcaagtggcg cgggtcgatc agaccaccag caccggattt ttcttggaaa
      181 cccggctgta gaggtcgatg atgtcctggt tgatcaggcg tacgcagccc gacgacatgg
      241 cctggccgat cgaccaccat tccggtgagc cgtggatgcg gtagccggtg tccttggcgt
      301 cctggaaaag atagagcgcg cgcgcaccga gcggattttg caggccgccg ggctggccgc
      361 cctgccactt caccagttcg ggcttgcgct ggatcatttc cggcggcggc gtccaggtcg
      421 gccattcgcg tccatattgc acattggcgc ggccggacca gctgaagcca tccttgccga
      481 tgccgacgcc gtagcggatg gcctcgccgc cttcctgcac cagatagagg tggcgctcct
      541 gaagacggac gacgatggtg ccgggccgtt cgccggtagg atcgacgacg atctggcggc
      601 ggaattccgg cttcaccttc tggtagggca ccgccggcac gccaaagccg ccatccgtca
      661 acccggcata catcacgtcc gggctggtga tgtgcttgtc gacgctgatg ctcggccgga
      721 tcgggcggat cgaaccggtg ctcatattgt cgagttggag cgccgacatg tcgacgctgt
      781 gcgtgcagcc gctggcggtt agcatcgcca tggcgccggc gccgaaaaga accgtgcggc
      841 gggataggaa tttttcggct cccgccggtt cgccgatttc cggcgatatg tcgtctagct
      901 ggaactcacc catgcccaca ccctaacgct gtcggcgcgg acacgcggtc cggccagatg
      961 gatgcatttt ccgcaatcat ggttgataaa gcgtgaagat accgatgcgc ctgcaactac
     1021 aagtggtccg gtgctcattt tggtctttac atattcgctg gacgcaggat ttatcctttt
     1081 ctttgcgctc caaggagaca cgcatgacca tcgagatatg ggccgccttt tgcgccgcat
     1141 cgatgattct cctgattatt ccggggccga ccatcctgtt ggtcgtttcc tatgcgcttg
     1201 gccagggctg gcgcatcgcg ctgccgatgg cggccggcgt ggcgctcggc gatttcaccg
     1261 cgatgacgct ttcgatgctg ggcatcggcg cgctgctggc ggcttcggcg accgttttca
     1321 ccatcctcaa gctgatcggc gccgcctatc tcgtctatct cggcatcaag ctgtttcgcg
     1381 ccggcggcgc gttgcgggcc gaaccgcgca ccgatgcgag gtcggcggcc aggatgatgg
     1441 cgcatgcctg cctggttacc gcgctcaacc cgaaaagcat caccttcttc gttgccttcc
     1501 tgccgcaatt cctcgaccgg aacggcgatt tctggatgca gatgctgatc ttcgaggcga
     1561 cattcctgac cttggccttt gccaacgcct tcggctacgc gctggtggcg gcgcgcgccc
     1621 gcacgcttgt cggcaaccag aaagcgatcc gcgtcttcaa ccgcaccggc ggcaccctgc
     1681 tggtcggcgc gggcatcgcc accgtcgcca tccgctccgg cggttgaggc gagcgggcta
     1741 gccggaatta ttccgagccg cgaagaaact ggccatggcc agtttccagg ccgaagtttt
     1801 tgctggtagg gtgagcgtcg cgggcgattg cccgccgtca gctgcccact cacaagcccc
     1861 tccccgccac tcaccaatga aaaggaagcg caatgtatct cggccttgat ctcggcacct
     1921 ccggcgtcaa ggcgctgctg atcgacgctt cgcaaaagat catcggggcc ggccatgccg
     1981 cgctggatgt ggcgcggccg catcccggct ggtcggaaca ggacccggcc caatggattc
     2041 gcgcctgcga ggaagccatc ggcgagttga aggcaacgca cgggaaagag cttgcggcgg
     2101 tgaaaggcat cggtctttcc ggccagatgc acggcgcgac gctgctcgac cgtgacgaca
     2161 aggtgctgcg cccctccatc ttgtggaacg atacgcgcag ccacgccgaa gccgccgcgc
     2221 tggacgccga tccccgcttt cgcgccatca ccggcaatat cgtctttccg ggcttcaccg
     2281 cgcccaagct ggcatgggta aggaacaacg aggccgggct tttcggcaag gtggccaagg
     2341 tgctgctgcc gaaggacttc ctgcggctgt ggctgaccgg cgagcatatt tccgaaatgt
     2401 cggacgccgc cggcacctca tggctcgaca ccggcaagcg gcgctggtcg cccgaattgc
     2461 tggccgcgac cttccttgac gaaaagcaca tgccgaccct tgtggaaggc actgaaaagg
     2521 ccggcgcgct gcgcggcgaa ctggcggcaa aatggggcat ggcggccggc atccccatcg
     2581 ccggcggggc cggcgacaat gcggcttcgg cctgcggcat gggcacggtc ggcgaaggcc
     2641 atgccttcgt ctcgctgggc acatccggcg tcttgtttgc cgccaacggt tgctacctgc
     2701 ccaatccggc cagcgccgtg cacactttct gccacgcgct gcccaacacc tggcatcaaa
     2761 tgggcgtcat cctttcggca accgattcac tgaactggct gtctgaaatc accggcaagg
     2821 atgcacgcgc gctgaccgac gaactgggcg acacgctgaa agcgccgacc ggcgtttcgt
     2881 tcctgcctta tctctcgggc gaacgcacgc cgcacaatga ttccgccatt cgcggctcgt
     2941 tcaccgggct tgcccatgaa tcgggccgcc cggccctgac ccaggcggtg gtggagggcg
     3001 ttgccttcgc cttccgcgac agccttgaag ccttggcgaa agccggcacc accttgaccc
     3061 gcgtgacagc catcggcggc ggctcacgct cgcgctactg gctgaaggcg atcgccaccg
     3121 cactcggcat tccggtcgat attcccgccg acggcgattt cggggcggca ttcggcgcgg
     3181 cgcgccttgg cctgatcact gccaccgggg ccgatccgct cagcatatgc tctgcgcctg
     3241 caacggaagc gacgatagag ccggacggtg aactgcgtga tgcctatgcg caagcctacg
     3301 ggcgctatcg tgcgctctac cccgccataa agggcgctac agcataggcg ttcggctggt
     3361 tgggcggggc ggacaaaact ttgccccacg ccgccattca atcgcctatc tcttgccttc
     3421 atgaagccgt cacgcacctc ctatattctg gccatgcaac gaaccggaag aaaaggaggc
     3481 gaaacgatgc atcacgaaca cgaac
//
LOCUS       DASYCS010000130         1912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_753510,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000130 DASYCS010000000
VERSION     DASYCS010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753510"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1294
                     /locus_tag="VGM46_04450"
     CDS             <1..1294
                     /locus_tag="VGM46_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015314555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY4191874.1"
                     /translation="QAEAYDYPESFFAERIWNQRRTRPDRLELAMAAAALKAAKKPLI
                     IAGGGALYSQASGELAKFAQGAGIPVCETQGGKSSLPDDHPLNMGAVGVTGTSAANRL
                     AEEADVVLAVGTRLQDFTTGSWSLFKNAGKTIIGLNVQPFDAGKHRALPLVADAAEGL
                     AELAMALQGWKAPAAWTENSARWKKEWQGEAARVTAPTNAPLPSDAQVIGAVQRACGS
                     EVIILHAAGGLPGELHKLWQAGSPGSYHAEYGFSTMGYEIAGGLGVKMARPDAEVVVM
                     VGDGSYLMLNSEIATSVMLGLKLTIVLLDNRGFGCINRLQVATGGASFNNLLKDARHE
                     TLPDIDFAAHAASLGAISQKVSSVAQLETALADARKHGAAGNARTTVLVIDTDPLVST
                     DAGGHWWDVAVPQVSERSQVNAARKAYDEQRRAQKLGD"
     gene            1316..>1912
                     /locus_tag="VGM46_04455"
     CDS             1316..>1912
                     /locus_tag="VGM46_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018857447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEY4191875.1"
                     /translation="MKAKLGMSPIAWWNDDLAELSDDVSLEECLAQSRSAGFTGMEMG
                     RRFPSDPKLMLPILKQADVTLCGGWFSGTLVNEEMAVNRDRIQPMIELFKAVRAPCIV
                     YGEVGRSIQGDRSKPLSTKPRLSDAEMKAYGKKLTEFGEWCAGQGMPLAYHHHMGAVV
                     ETETELDAFMNHSGPGIPLLLDAGHLAFAGGDVLRAIEN"
BASE COUNT          360 a          588 c          644 g          320 t
ORIGIN      
        1 gcaggccgag gcctacgact atccggaaag cttttttgcc gagcgcatct ggaaccagcg
       61 ccggacgcgc ccggaccggc tggaattggc gatggcggcg gcggcattga aagccgcgaa
      121 aaagccgctg atcatcgccg gcggcggcgc gctttactcg caagcctcgg gcgaactggc
      181 gaagtttgcg caaggcgccg gcattccggt ctgcgagacg cagggcggca aatcctcgct
      241 gcccgacgat catccgctga acatgggcgc tgtcggcgtc accggcacct ccgccgccaa
      301 ccggttggcc gaggaggccg atgtcgttct ggcggtcggc acgcgcctcc aggatttcac
      361 caccggatcg tggtcgctgt tcaagaacgc cggcaagacc attatcggcc tgaacgtgca
      421 gcccttcgac gccggcaaac accgcgcgct gccactggtg gccgatgccg ccgaggggtt
      481 ggccgaactg gcgatggcgc ttcaaggctg gaaagcgccg gccgcgtgga ccgaaaattc
      541 agcccggtgg aaaaaggaat ggcaaggcga ggcggccagg gtgacggccc ccaccaacgc
      601 gccactgccc tcggatgcgc aggtgatcgg cgcggtgcag cgcgcctgcg ggtcggaggt
      661 aatcattctg cacgccgccg gcggcctgcc gggcgaattg cacaagttgt ggcaggcggg
      721 gtcgcccggt tcctatcacg ccgaatacgg cttctcgacc atgggctatg agattgccgg
      781 cggactgggc gtcaagatgg caaggcctga tgccgaggtc gtggtcatgg tcggcgacgg
      841 ctcctacctg atgttgaatt ccgaaatcgc cacttcggtg atgctcggcc tgaagcttac
      901 catcgtgttg ctcgacaatc gcggtttcgg ctgcatcaac cggttgcagg tggcgacggg
      961 cggcgcgagc ttcaacaatt tgctgaagga tgcgcgccac gaaacgctgc ccgacatcga
     1021 tttcgccgcc cacgccgcca gccttggcgc aatttcgcaa aaggtctcgt cggtggcgca
     1081 gttggagacg gctctggccg atgcacgcaa gcacggtgcg gccggcaatg cgcgcacgac
     1141 ggtgctggtc atcgacaccg acccccttgt ctcgaccgat gccggcggcc attggtggga
     1201 tgtcgcggtg ccgcaggtga gcgagcgcag ccaggtcaat gccgcgcgca aggcctatga
     1261 cgaacagcgc cgcgcgcaga aactgggcga ttgaggtaga aaccggatga gcggattgaa
     1321 ggccaagctg ggcatgtccc cgatcgcgtg gtggaacgac gatcttgccg aattgagcga
     1381 cgatgtttcg ctggaagagt gcctcgcgca atcacgttcg gcaggcttca ccggcatgga
     1441 aatgggccgg cgctttccga gcgatccgaa gctgatgctg ccgatcctca aacaggccga
     1501 tgtgacgctt tgcggcggct ggttttccgg cactctcgtc aatgaggaga tggcggtaaa
     1561 cagggaccgc atccagccga tgatcgaact gttcaaggcg gtccgcgcgc cctgcatcgt
     1621 ctatggcgag gtcggccgct ccatccaggg cgaccgctcg aagccgctga gcaccaagcc
     1681 gaggctgtcc gatgccgaaa tgaaggcata tggaaaaaaa ctgacggaat tcggcgaatg
     1741 gtgcgccggg cagggcatgc cgctcgccta tcatcaccac atgggggcgg tcgtcgagac
     1801 cgagacggaa ctggacgcct tcatgaacca ttcaggccca ggcatccccc tgctgctcga
     1861 tgccgggcat ctggcgtttg ccggcggcga cgtgctgcgc gccatcgaaa ac
//
LOCUS       DASYCS010000131         3880 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_753548,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000131 DASYCS010000000
VERSION     DASYCS010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753548"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1951
                     /gene="gcvP"
                     /locus_tag="VGM46_04460"
     CDS             <1..1951
                     /gene="gcvP"
                     /locus_tag="VGM46_04460"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEY4191876.1"
                     /translation="RLVGQSNDSKGRPGYRLALQTREQHIRRDKATSNICTAQALLAN
                     MATAYAIWHGPAGLQAIAARIHAMAGRLADGLDAAGLKLIGSRRFDTVTVETKGKAGE
                     IAAAAEKSGRLLRIVDADHIGIAFDETSTEADVQAIAALFGARIADDTTGKMPGKPRE
                     KEFLTQPVFHENRSETEMMRFLRRLADKDLALDRTMIPLGSCTMKLNAAAEMMPVSWP
                     SVANLHPFAPKTHSIGYRAMIGQLEGWLSEITGFDAVTLQPNAGSQGEFAGLLAIRGY
                     HRARGESHRTVCLIPSSAHGTNPASAAMAGMRVVVVSCTQDGNIDMDDLKAKAEEHAG
                     ELAALMFTYPSTHGVFEEGARDICAIVHEHGGQVYFDGANLNALVGLSRPGDIGADVC
                     HMNLHKTFCIPHGGGGPGIGPIGVKAHLKPYLPGHVVEGSAHAVSAAPFGSASILPIT
                     WMYIRMMGASGLKQATEAAIVSANYIAARLDACFPVLYKGRSGRVAHECILDTRVVKE
                     SAGIGVEDIAKRLIDYGFHAPTMSFPVAGTLMVEPTESEPKRELDRFCEAMIAIAGEA
                     EKVASGTWPLADNPLVNAPHTASETLAGDWNHPYSRMEAAFPAGDADSAAKYWPPVSR
                     IDNVAGDRNLVCSCPPLADYLGAAE"
     gene            2012..2656
                     /locus_tag="VGM46_04465"
     CDS             2012..2656
                     /locus_tag="VGM46_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HEY4191877.1"
                     /translation="MIRLDKKLRFFAASLSFVAGFVDALGFIHLGGYFVSFMSGNSTR
                     MAVSLSERPADGLLPLSLMGLFLAGVVGGTILSSRLNGRHGRVMLLSVSGLLAVTALL
                     ATLGLGRMAIWITPVAMGAMNTLFQRDGEVSVGVTYMTGTLVKLGQRLAAALTGGPRW
                     AWAPYLLLWLGLMIGAICGAAAYPRFGLVALWLPVAILLALSGLSGQIDRANRG"
     gene            2736..>3880
                     /locus_tag="VGM46_04470"
     CDS             2736..>3880
                     /locus_tag="VGM46_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY4191878.1"
                     /translation="MDDAKDLFGGLDKQPQTPVRAPSRPLDPMVLALQKSAAKRAAPM
                     LDGSEGYSAADIEVLEGLEPVRRRPGMYIGGTDDKAMHHLFAEVIDNSMDEAVAGHAT
                     FIDVELSADGFLSVTDNGRGIPVDMHPKYNKPALEVIMTTLHSGGKFDSKVYETSGGL
                     HGVGVSVVNALSDHLEVEVARGRQLYRQRFSRGIAVSELEKLGDVHNRRGTRIRFHPD
                     EQIFGKGAAFEPARLYRMTRSKAYLFGGVEIRWSCDPSLIKEKDETPAKANFHFPGGL
                     KDYLASSLGDDFQVTREIFAGKSEKQGGHGSLEWAVTWFGGDGFLRSYCNTIPTGEGG
                     THEAGFRNVLTRGLRAYADLVGNKRASIVTSEDVMISAAGMLSVFIR"
BASE COUNT          707 a         1302 c         1175 g          696 t
ORIGIN      
        1 ccgcctcgtc ggccagtcca atgacagcaa gggccgtccc ggctaccgcc tcgccctgca
       61 aacacgcgaa caacatatcc gccgcgacaa ggcgacctcc aacatctgca ccgcgcaggc
      121 gctgcttgcc aacatggcca ctgcctacgc gatctggcac ggcccggccg gcttgcaggc
      181 gattgccgcg cgcatccacg cgatggccgg ccgccttgcc gacggcctgg acgccgccgg
      241 cctgaagctt atcggctcgc gccgcttcga tacggtgacg gtcgagacca aaggcaaggc
      301 cggggaaatt gccgccgccg ccgaaaaatc cggccgcctg ctgcgcatcg tcgatgccga
      361 ccacatcggc atcgccttcg atgaaacctc gaccgaggcc gacgttcagg cgatcgccgc
      421 gctgttcggc gccaggattg ccgatgacac caccggcaaa atgccaggaa aaccgcgcga
      481 aaaagaattc ctgacgcagc cggtcttcca cgaaaaccgt tccgaaaccg agatgatgcg
      541 cttcctgcgc cggcttgccg acaaggatct ggcgcttgac cgcaccatga tcccgctcgg
      601 ctcctgcacc atgaagctca atgccgccgc cgagatgatg ccggttagct ggccaagcgt
      661 tgccaatttg catcctttcg cgccaaaaac ccactccatc ggctaccgcg ccatgatcgg
      721 ccagctggaa ggctggctgt cggaaatcac cggcttcgac gccgtgacct tgcagcccaa
      781 cgccggcagc cagggcgagt ttgccgggct gctcgccatt cgcggctacc accgcgcgcg
      841 cggcgaaagc caccgcaccg tctgcctcat cccctcttcc gcccacggca ccaatccggc
      901 aagcgccgcc atggccggca tgcgcgtcgt cgtcgtgtcc tgcacgcagg acggcaatat
      961 cgacatggat gatttgaagg ccaaggctga agagcatgca ggcgaactgg ccgccttgat
     1021 gttcacctac ccgtcgacgc atggcgtgtt cgaggaaggc gcgcgcgaca tctgcgccat
     1081 cgtgcacgag catggcggcc aggtctattt cgacggcgcc aatctcaacg cgctggtcgg
     1141 cctgtcgcgg cccggcgaca ttggcgcgga tgtctgccac atgaacctgc acaagacctt
     1201 ctgcattccc catggcggcg gcggtccggg catcggcccg atcggcgtca aggcgcatct
     1261 gaagccctat ttgcccggcc acgtcgtgga aggctcggcc catgccgtgt cggccgctcc
     1321 gttcggcagc gcctccattc tgccgatcac ctggatgtat atccgcatga tgggcgcatc
     1381 cggactgaaa caggcaaccg aagccgcaat cgtttccgcc aattacatcg cggcacggct
     1441 cgatgcctgt tttccagtgc tctacaaagg gcgcagcggg cgcgtggcgc atgaatgcat
     1501 cctcgatacg cgcgtggtga aggaaagcgc cggcatcggc gtcgaggata tcgccaagcg
     1561 cttgatcgac tacggcttcc atgcaccgac catgtcgttc ccggtagccg gcacgctgat
     1621 ggtggagccg accgaatcgg aacccaaacg cgaactcgac cgcttctgcg aggcgatgat
     1681 cgccattgcc ggcgaagccg aaaaggtggc gagcggcaca tggcccttgg ccgacaaccc
     1741 ccttgtcaac gccccgcaca ccgcatccga aacgctggcc ggcgactgga accaccccta
     1801 ttcccggatg gaagcagcct ttccggcagg cgatgccgat agcgcggcca aatactggcc
     1861 gcccgtctcg cgcatcgaca atgtggccgg cgaccgcaac ctcgtctgct cctgcccgcc
     1921 gctggcggat tatctgggtg cggcggagta ggaaacaggc tagcaagcac cggaaacggc
     1981 gcgtcgcggc gcgcgccgca atggttgcat catgatccgg ctggataaga aattacgctt
     2041 cttcgcggcc agcctgtcct tcgtggcagg cttcgtcgac gcgcttgggt tcatccatct
     2101 cggcggttat ttcgtgtcgt tcatgagcgg caattccacc cgcatggcag tcagcctatc
     2161 cgaacgccct gcggacggcc tgctgccgct gtcgcttatg ggcctgttcc ttgccggcgt
     2221 cgttggcggc acgattcttt caagccgatt gaacggacgc catggcaggg tcatgctgtt
     2281 gtccgtttcg ggactgcttg ccgtgacagc cctgttggcc accctcgggc ttggccgcat
     2341 ggcgatttgg ataaccccgg tcgccatggg tgcgatgaac accttgttcc agcgcgacgg
     2401 tgaagtcagc gtcggcgtca cctacatgac cggcacgctg gtcaagctcg ggcaacgcct
     2461 ggcggcagcc ttgaccggag gtccgcgctg ggcctgggcg ccttatctgc tgttgtggct
     2521 cggcctgatg atcggcgcca tctgcggtgc ggcggcctat ccgcgtttcg gcctcgtggc
     2581 tctttggctg ccggttgcta ttttgctggc gctaagcggc ctttcgggcc agatcgaccg
     2641 ggccaatcga ggctgagcgg ccgccacgct gtcgcttcga ccctaaaacg tggcaatatg
     2701 gaacgtgatt cgcacattcg ggaattccag aacatatgga tgacgccaaa gaccttttcg
     2761 gcggccttga taagcagccg caaaccccgg tccgcgcccc ttcccggccg ctggacccga
     2821 tggtgctggc attgcagaag tcggccgcaa agcgcgccgc gcccatgctg gacggcagcg
     2881 aaggctatag cgccgccgac atcgaggtgc tggaggggct ggaacccgtg cgccggcggc
     2941 ccggcatgta tattggcggc accgacgaca aggcgatgca ccacctgttt gccgaggtca
     3001 tcgacaattc aatggacgag gcggtcgccg gccatgccac cttcatcgac gtggaattgt
     3061 cggccgacgg cttcctgtcg gtcaccgata atggtcgcgg catccccgtc gacatgcacc
     3121 cgaaatacaa caagccggcg ctcgaagtca tcatgacgac gctgcattcg ggcggcaagt
     3181 tcgattcgaa ggtctacgaa acgtcgggcg gcctgcatgg cgtcggcgtt tcggtggtca
     3241 acgcgctgtc cgaccatctc gaagtcgaag tggcgcgcgg ccgccagctc taccgccagc
     3301 gcttttcacg cggcatcgca gtcagcgaac tagaaaagct tggcgacgtg cacaatcggc
     3361 gcggcaccag gatccgcttc caccccgacg agcaaatctt cggcaagggc gcggctttcg
     3421 aaccggcccg cctctaccgc atgacgcgtt cgaaagccta tctgttcggc ggcgtcgaaa
     3481 tccgctggtc ttgcgatcct tcgctcatca aggagaagga cgagacgccg gccaaggcca
     3541 acttccactt tccaggcggc ctcaaggatt atctggcctc ctcgctcggg gacgatttcc
     3601 aggtcacgcg cgaaatattc gccggaaaaa gcgaaaagca gggcggccac ggctcgctcg
     3661 aatgggcggt cacatggttt ggcggcgacg gcttcctgcg ctcctactgc aacaccatcc
     3721 ccaccggtga aggcggcacg cacgaggccg gcttccgcaa tgtgctgacc cgaggccttc
     3781 gcgcctatgc cgatctggtc ggcaacaagc gcgcctccat cgtcacttcg gaagacgtga
     3841 tgatttcggc tgccggcatg ctttcggtct tcatccgcga
//
LOCUS       DASYCS010000132         7151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_318308,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000132 DASYCS010000000
VERSION     DASYCS010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 7151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7151
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318308"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VGM46_04475"
     CDS             complement(<1..209)
                     /locus_tag="VGM46_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191879.1"
                     /translation="MAETPKVTNVKASDIQDAMRKQIAELRREMNRMNKTMAERAEDV
                     SEQASGWYDSASEGASRAARQLRTRA"
     gene            complement(278..445)
                     /locus_tag="VGM46_04480"
     CDS             complement(278..445)
                     /locus_tag="VGM46_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Thivi_2564 family membrane protein"
                     /protein_id="HEY4191880.1"
                     /translation="MAGSLLIGILITFLVIVLVLYLVQRLPLDGRTRQIAQIVVIIIG
                     IISLLKYLSVF"
     gene            complement(535..1848)
                     /locus_tag="VGM46_04485"
     CDS             complement(535..1848)
                     /locus_tag="VGM46_04485"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010659878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEY4191881.1"
                     /translation="MAEALRVGIAGLGTVGASVARVLRDKAAELTRQCGREIVVSAVS
                     ARDAKRDRGVNLSAVKWFDDPVKLAQSADIDVFVELIGGDEGPARACVKAALEAGHHV
                     VTANKALLAKHGVALAEIAEKKGVLLNYEAAVAGGIPVIKTMREAMAGNSVSRVFGIL
                     NGTCNYILTRMEAEGLSFEDCLKDAQRLGYAEADPTFDIEGNDTAHKLAILTSLAFGT
                     KIAADSIYLEGISNITQADIRAAGELGYRIKLLGVAQRTESGIEQRVHPTMVPTASVI
                     AQVNGVTNAVAIETDILGELLLSGPGAGGNATASAVIGDIADIAKSRPGFQHGPVFGR
                     PAKELAPYKKAQMRAHAGGYFIRLTVHDRIGVFAAIAKRMAENDISLESIVQHAAASE
                     EVLQKTVILVTHETTESAVRKAVEGITKDGHLTSKPQVIRIERAG"
     gene            complement(1898..3115)
                     /locus_tag="VGM46_04490"
     CDS             complement(1898..3115)
                     /locus_tag="VGM46_04490"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HEY4191882.1"
                     /translation="MEEFHKVRRLPPYVFEQVNRLKASARARGADIIDLGMGNPDLPT
                     PKAIVDKLCEVVRDPRTHRYSSSRGIPGLRRAQAAYYARRFGVKLNPETQIVATLGSK
                     EGFANMAQAITAPGDVILCPNPTYPIHAFGFIMSGGVIRSLQVEPDEKFIPALERGMR
                     HSIPKPLALILNYPSNPTALVASLDFYKDVVAFAKKNDLIILSDLAYSEIYFDGNPPP
                     SVLQVPGAMDVAVEFTSMSKTFSMPGWRMGFAVGNERLISALTRVKSYLDYGAFTPIQ
                     VAASAALNGDGTDIEEVREVYHKRRDVMVDAFGRAGWTIPAPAASMFAWAPIPEPFRH
                     LGSLEFSKLLIEHADVAVAPGVGFGEHGDDFVRLALVENEHRIRQAARSIKRFLSTKA
                     GEPDNVIALSAHR"
     gene            complement(3190..3522)
                     /locus_tag="VGM46_04495"
     CDS             complement(3190..3522)
                     /locus_tag="VGM46_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006335072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191883.1"
                     /translation="MAAALLTLAGCGGGNGAGNAVPVSQGARDTGTFPNLNIRPQVAA
                     EQFTDSERDAKLGQLKAQQAQAIASPGATTETADAASLNKLAASHANDTLKQIEGKCD
                     PALDPTCK"
     gene            3677..5512
                     /gene="phaC"
                     /locus_tag="VGM46_04500"
     CDS             3677..5512
                     /gene="phaC"
                     /locus_tag="VGM46_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009451740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HEY4191884.1"
                     /translation="MADAKEPHKTAEADASPVDQYLVKDPESFALNIARMVEQAGKAA
                     SAWVEPREKGEMRDHVAEPAVDMVKTFSKLSEYWLSDPQRALEAQTRLFAGYMGVWAN
                     AIQRAGAGVGESGDTVQPERGDKRFQDPEWGRNAFFDFLKQAYLVTSRWAADLVEHAD
                     GLDEHTRHKAGFYVKQVSNAISPSNFILTNPELFRETVASNGENLVRGMKMLAEDIAA
                     GKGDLKLRQADQTTFEIGRNLAMTPGKVVGRSEVAEIIQYEPTTKTVLKRPLLICPPW
                     INKFYILDLNPQKSFIRWAIEQGHTVFVISWINPDERHGAKDWEAYIREGLQYGLDIV
                     ETATGEHEVNAIGYCVGGTLLAAALALLAQEGDHRIQSATLFTTQVDFTHAGDLKVFV
                     DEEQVAAVERSMSEKGYLDGTKMATAFNMLRSGDLIWPYVVNNYMRGKDPLPFDLLYW
                     NADSTRMAAANHSFYLRNCYLENNLSLGRMELAGRTVSLGDIAIPVYNLATKEDHIAP
                     ARSVFLGCRYFGGPVEFVMAGSGHIAGVVNPPAANKYQYFTGGKPVGDFDDWLAKATQ
                     NPGSWWPHWQKWIEEKGDARVPARLPGANLQTLGEAPGSYVKVRV"
     gene            5758..6903
                     /locus_tag="VGM46_04505"
     CDS             5758..6903
                     /locus_tag="VGM46_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009451739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase family
                     metallohydrolase"
                     /protein_id="HEY4191885.1"
                     /translation="MAVLFSVLLASCTSAGNPPLSLGMADYTADGGISADASIASNDS
                     QTAQVAGPQALMTAEGDAELPAEIAYLPSPSPGFGFPIAAPAGVETIAGNTPASLQPA
                     TVPSTASPTAMAEAAADVPAKTAEPEDAASKPVLDNPVYVTAGEMPKPKVATAPKKKS
                     LFASLFGTSPAEAAPAPLVEVNQEREMVKPVITLASAEVDEKPLINPDEVAHASVNGA
                     LPGVRQTALFEIKRKSGIDDESDVDLNEDEGPGLYKVASAAGMARLAPNGLLKQTESV
                     DVACLKPSLVRVLKTIENHYHEKMIVTSGYRDPNRNRRANGARNSMHMYCAAADIQLP
                     GISKWELASYVRSMPGRGGVGTYCHTESVHVDVGPERDWNWRCRRRK"
     gene            7077..7151
                     /locus_tag="VGM46_04510"
     tRNA            7077..7151
                     /locus_tag="VGM46_04510"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7110..7112,aa:Glu,seq:ttc)
BASE COUNT         1406 a         2242 c         2164 g         1339 t
ORIGIN      
        1 gctctcgtcc gcaactgcct ggcggcgcgc gatgcgcctt cggatgcgct gtcgtaccaa
       61 ccggaggcct gttcggaaac atcctcggcc cgctccgcca tcgtcttgtt catcctgttc
      121 atttcgcggc gcagttcggc gatctgcttg cgcatcgcat cctggatgtc gcttgctttg
      181 acattggtga ctttgggcgt ttcggccatg tgaaattcct ttcgttttca aggtaacggc
      241 cgctggccgc actcgttcca atgcttaaag ccacgcccta gaagacggaa agatacttca
      301 gcagcgatat gatgccgatg atgatgacga caatctgcgc gatctggcgc gtgcgcccgt
      361 ccaaaggcaa tctctggacg agatacagaa ccaggacgat aaccagaaag gtaataagga
      421 tgccgattaa aagacttcca gccatgttgt ccctcacttg atgatgcttc aaacaaggcg
      481 aataacggct tcaaccggct aaaagttccg ttggctacac cagcaggcta tgcgctatcc
      541 ggcgcgctcg atccggatca cctgcggctt gctcgtcagg tggccgtcct tggtgatgcc
      601 ttcgaccgcc ttgcgcacgg cggattccgt tgtctcatgc gttaccagga tcaccgtctt
      661 ctgaaggact tcctccgagg ccgcggcatg ctggacgatt gattccagcg aaatatcgtt
      721 ttcagccatc cgcttggcga ttgccgcaaa cacgccgatg cggtcgtgaa cggtcaggcg
      781 gatgaagtag ccgcccgcat gcgcgcgcat ctgcgctttt ttgtagggcg cgagttcctt
      841 tgccgggcgt ccaaagaccg gcccatgctg gaagcccgga cggctcttgg cgatgtcggc
      901 tatgtccccg atgacggccg aggcggtggc attgccgccc gcgcccggac ccgaaagcag
      961 aagctcgccc agaatgtcgg tttcgatcgc caccgcgttg gtgacgccgt tgacctgcgc
     1021 aatgacggag gcggtcggca ccatggtcgg atgcacgcgc tgctcgatgc cgctttcggt
     1081 gcgctgggcg acgccgagca gctttatccg gtagccaagc tcgcccgccg cgcgaatgtc
     1141 ggcctgcgtg atgttggaaa tgccttccag gtagatgctg tcggcggcga ttttcgtgcc
     1201 gaaggcaagg ctggtcagga tggcaagctt gtgcgcggtg tcgttgcctt cgatgtcgaa
     1261 ggtcggatcc gcctccgcat agcccagccg ctgggcgtcc ttcaggcaat cctcgaagga
     1321 caggccttcg gcctccatgc gcgtcagtat gtagttgcag gtgccgttca aaatgccgaa
     1381 cacgcgcgac acgctgttgc cggccatcgc ctcgcgcatc gtcttgatga cgggaatgcc
     1441 gccggccacg gctgcctcgt agttcagcag cacgcccttc ttttcggcga tttcggccag
     1501 cgccacgccg tgcttggcaa gcagagcctt gttggcggtg acgacgtggt ggccagcttc
     1561 gagcgccgcc ttcacgcagg cgcgcgccgg cccctcatcg ccgccgatca actcgacgaa
     1621 aacgtcgata tccgccgact gggcaagctt gaccggatcg tcgaaccatt tgacggcgct
     1681 gagattcacg ccccggtccc gtttggcgtc gcgcgccgaa acggccgaaa ccacgatttc
     1741 gcggccgcat tggcgggtaa gctccgccgc cttgtcacgc aacacgcgcg ccaccgacgc
     1801 gccaacggtg ccaagaccgg caattccaac acgcaatgct tcagccatgg tcggcggcgt
     1861 ccctcaagtt caagaaatca agctatggcg acgccgctca gcgatgggcc gacagagcga
     1921 tgacattgtc cggttcgccg gctttcgtcg acaagaagcg cttgatgctg cgcgccgcct
     1981 gccggatgcg atgctcgttc tcgaccagcg ccaggcgcac gaaatcgtcg ccgtgctcac
     2041 caaatccaac gccaggcgcc accgccacat cggcatgttc gatcagcagc ttggagaatt
     2101 ccagcgaacc aagatgccgg aacggctcgg ggatcggcgc ccatgcaaac atcgacgcgg
     2161 ccggcgccgg tatggtccaa ccggcgcggc cgaaggcgtc aaccatgacg tcgcggcgct
     2221 tgtgataaac ctcgcgcacc tcttcgatat cggtgccgtc gccattcagc gccgcagatg
     2281 cggcgacctg gatcggcgtg aacgcaccat aatcgagata ggatttcacc cgcgtcagcg
     2341 ccgagataag gcgctcgttg ccgaccgcga agcccatgcg ccagcccggc atggagaagg
     2401 ttttggacat ggaggtgaat tccacagcga cgtccatcgc gcccggcacc tgtagcaccg
     2461 atggcggcgg gttgccgtcg aaataaatct ccgaataggc aaggtccgac aggatgatga
     2521 ggtcgttctt cttcgcgaac gcgaccacgt ccttgtagaa atcgagcgag gcgaccagcg
     2581 ccgtcgggtt cgacggataa ttgaggatca gcgccagcgg cttggggatc gaatggcgca
     2641 tgccgcgctc aagcgctgga atgaactttt cgtctggctc cacctgtagc gagcggatga
     2701 cgccacccga catgatgaag ccgaaggcgt ggatcgggta ggtcgggttc gggcacagga
     2761 tcacgtcgcc gggcgcggtg atggcctgcg ccatattggc aaagccctct ttcgaaccga
     2821 gcgtcgccac gatctgggtt tcggggttga gtttcacccc gaagcggcgg gcgtaatagg
     2881 ccgcctgcgc ccgccgcagg cccggaatgc cgcgcgagga agaatagcgg tgcgtgcgcg
     2941 ggtcgcgaac gacctcgcat aatttgtcga cgatggcttt cggcgtcggc agatcgggat
     3001 tgcccatgcc aaggtcgata atgtcggcgc cacgcgctcg cgcgctggcc ttcagccggt
     3061 tgacctgttc gaaaacatag ggcggcaaac gcctgacttt atgaaactct tccatggcaa
     3121 atccctgccg acaatcggca cgctctggag cacgctttcc gggcgcgctt tcggttgcgc
     3181 gctataaact tatttgcagg ttgggtcgag tgcggggtcg catttaccct cgatctgctt
     3241 cagcgtgtcg ttggcatggc tggcggccag tttgttgagc gaagccgcat cggcggtttc
     3301 cgtcgttgcg ccgggcgatg ctatagcctg cgcctgctgc gccttgagct ggccgagctt
     3361 ggcgtccctc tcggaatcgg tgaactgctc ggcggcaacc tgcggccgga tgttgaggtt
     3421 gggaaaggtg cccgtatcac gcgcgccctg cgaaaccggc acggcattcc ctgccccgtt
     3481 gccgccgccg caaccggcaa gcgtcagaag agcagcggcc accgcgagcc gaaaaattcc
     3541 cgcgcccgag agaaacgcag tttcaacacc atatgtcata ttgtttccct ggcgaacgag
     3601 gtagagccat tggaccggga ttgccgtccc gtggtaatat gtcaagaaaa cacatcggga
     3661 ggaaccactc aaacctatgg ccgacgcgaa ggagcctcac aagacggccg aagccgatgc
     3721 ttcgccggtc gaccagtatc tggtgaagga cccggaaagt ttcgcgctga acatcgcgcg
     3781 catggtcgag caggctggca aggccgcgtc cgcatgggtg gagcctcgcg aaaaaggcga
     3841 aatgcgcgac catgtcgccg aaccggccgt cgacatggtc aaaaccttct ccaagctcag
     3901 cgaatactgg ctttccgacc cgcagcgcgc gctggaagcg cagacgcggc ttttcgccgg
     3961 ctatatgggt gtctgggcca acgccatcca gcgcgccggc gcgggcgtcg gggaatcggg
     4021 cgatacggtc cagcccgagc gtggcgacaa gcgcttccag gaccccgaat ggggccgcaa
     4081 cgcattcttc gattttctga agcaggccta tctcgtcacc tctcgctggg cggccgacct
     4141 ggtcgagcac gccgatggcc tggacgagca cacccgccac aaggctggct tctacgtcaa
     4201 gcaggtgtcc aacgccatct cgccatcgaa cttcatcctg acaaatcccg aactgtttcg
     4261 cgaaaccgtc gcctccaacg gcgaaaacct cgttcgcggc atgaagatgc tggcggagga
     4321 catagcggcc ggcaagggcg acctcaagct caggcaggcc gaccagacga ctttcgagat
     4381 cggcaggaac cttgccatga cgccgggcaa agtcgtcggc cgcagcgagg tggcggaaat
     4441 catccagtac gagccaacga ccaagaccgt gctcaagcgt ccgctactga tttgcccgcc
     4501 ctggatcaac aaattctaca tcctcgatct caacccgcag aaatccttca tccgctgggc
     4561 catcgagcag ggccataccg tcttcgtcat atcgtggatc aacccggatg agcgccatgg
     4621 cgccaaggac tgggaggcct atatccgcga aggcctgcaa tatggcctag acatcgtcga
     4681 aaccgccacg ggcgagcatg aggtcaacgc catcggctat tgcgtcggcg gcacgctgct
     4741 ggcggccgcg ctcgccttgc tggcgcagga aggcgaccac cgcatccagt cggccacgct
     4801 tttcaccacg caggtggact tcacccacgc aggcgacctg aaagtgttcg tcgacgagga
     4861 acaggttgcc gcggtcgaac gctcgatgag cgaaaagggc tatctcgacg gcaccaagat
     4921 ggcgacggcc ttcaacatgc tgcgttccgg cgacctgatc tggccctatg tcgtaaacaa
     4981 ctacatgcgc ggcaaagacc ccctgccctt cgacctgctc tattggaatg ccgattccac
     5041 gcgcatggcg gcggcgaacc attcgttcta tctgcgcaac tgctacctgg agaacaacct
     5101 gtcgctgggc cgcatggaat tggccggacg caccgtatcg ctcggcgaca tcgccattcc
     5161 ggtctataat ctcgcaacca aggaagacca catagcgccg gcgcgctcgg tgtttctcgg
     5221 ctgccgctat ttcggcggcc ctgtcgagtt cgtgatggcg ggatcgggcc atatcgccgg
     5281 cgtcgtcaat ccgcccgccg caaacaagta ccagtatttc accggcggca agccggttgg
     5341 cgatttcgac gactggcttg ccaaggccac gcaaaacccc ggctcctggt ggccgcattg
     5401 gcaaaaatgg atcgaagaaa agggcgatgc ccgcgttccg gcccgcttgc ccggcgcaaa
     5461 tctgcaaacc ctcggcgagg ctccgggcag ctatgtcaaa gtgcgcgtgt aacagtctgt
     5521 aatgtgcggt gagttgacgg gcggccaaaa gctggcgaaa tgagcggcgg ctttatagcc
     5581 gatttatttc aagagttgct tatttagcac agttactgtc tgcggcttcc ggtgaccggc
     5641 ccgccgcaca atctggctcc aaccagaaga cgcggccaac gcgccgatgc caaaaggggg
     5701 aattctggtt tgaaatcagc aggatcgcgc cttgcgaaag aaaagggacg tgccgttatg
     5761 gccgtgcttt tttctgtgct cctggcatcc tgcacctctg cgggcaaccc accgctttcg
     5821 ctgggaatgg ctgattatac cgccgatggc ggcatttccg cggatgcgtc gattgcgtca
     5881 aatgattcgc aaacagcgca agtggctggc ccgcaggcgc tcatgactgc cgagggcgat
     5941 gccgaattgc cggccgaaat cgcttatctg ccgtcgccaa gcccaggctt cggtttcccg
     6001 atcgccgcgc ccgccggcgt tgagaccatc gcgggcaata cgccggcatc gttgcagccg
     6061 gccaccgtgc caagcacggc ttcgccaacg gcgatggccg aagccgcagc cgatgttcca
     6121 gccaagactg ccgagccgga agatgccgca tcgaaaccgg tgctggataa tccggtctat
     6181 gttaccgccg gcgaaatgcc gaaacctaag gtcgccacgg cgccgaagaa aaagagcctg
     6241 tttgcctcgc tgtttggcac atcgccggcg gaagccgcac ctgcccccct ggtggaagtc
     6301 aatcaggagc gcgagatggt gaagccggtt atcacgctgg cctccgccga ggtggacgaa
     6361 aaaccgctga tcaatcccga cgaggtcgcc cacgcttcgg tgaatggcgc cctgcccggc
     6421 gtgcgccaga cggctttgtt cgagatcaag cgcaagtccg gcatcgacga cgaaagcgac
     6481 gtcgacctga acgaggacga aggccccggc ctctataagg tggcttccgc cgccggcatg
     6541 gcgcggctcg cgcccaacgg cctgttgaaa cagaccgaaa gtgtcgacgt ggcgtgcctc
     6601 aagccctcgc tggtgcgcgt gctaaaaacg atcgaaaatc attaccatga gaagatgatc
     6661 gtcacgtccg gctatcgcga tccaaaccgc aatcgccgtg ccaacggcgc gcgcaattcg
     6721 atgcatatgt attgcgccgc cgccgatatc cagcttcccg gcatcagcaa atgggaactg
     6781 gcgagctatg tgcgttcgat gcctgggcgc ggcggcgtcg gcacctattg ccataccgaa
     6841 tcggtgcatg tcgatgtcgg gccggagcgc gactggaact ggcgctgccg ccggcgcaaa
     6901 taggtcatcc gccggcatcc ggtcatgctt ttttgcaggc tgaccatccc tgcgcgctac
     6961 aagacacgca accaccgccg aaataccggc atgatttgcc gaaaaaagtt atcttgagcc
     7021 cgagcaatgg gttgcccgtt ggcccggacg ccactataag ccgctttcat ctgagcgcgc
     7081 ccatcgtcta gcggtcagga caccgccctt tcacggcggt aacaggggtt cgattcccct
     7141 tgggcgtacc a
//
LOCUS       DASYCS010000133         3510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3653767,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000133 DASYCS010000000
VERSION     DASYCS010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3653767"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="VGM46_04515"
     CDS             complement(<1..887)
                     /locus_tag="VGM46_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006012385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEY4191886.1"
                     /translation="MPAVQDTIDRVRKIDVDQYKYGFETEIAMDKAPKGLSEDIIRFI
                     SAKKDEPDWMLEWRLEAYRRWLTLEEPTWARVHYPKIDFQDIFYYAAPKSTPGPTSLA
                     EVDPELLKTYAKLGIPLREQEILAGVQKPTAASEDDAAEGENDNVYKSGRVAVDAVFD
                     SVSVVTTFKKELAAAGVIFCSISEAIREHPELVRKYLASVVPISDNYYATLNSAVFTD
                     GSFVFVPKGVRCPMELSTYFRINEKGTGQFERTLIIAEEGAYVSYLEGCTAPQRDENQ
                     LHAAVVELIALDDAEIKYST"
     gene            complement(1080..2240)
                     /locus_tag="VGM46_04520"
     CDS             complement(1080..2240)
                     /locus_tag="VGM46_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEY4191887.1"
                     /translation="MAARRAYLDYNASAPLLPVARQAMLAALDASANPSSVHAEGRAA
                     RQMIEAARRSVAALVNGRPDHVVFTSGATEAASMLLTPDWRMGRAALRMGHLYVCEAD
                     HPCLLNGGRFPAEQLSRIGVDGEGIVRLDELAGALASHDKANGLPLVAIHAANNETGV
                     IQPVEKISAIVKAAGGVLVLDAAQAAGRIPLDIAAGYADCLILSAHKMGGPQGAGAIV
                     AAADLMMPAPLIAGGGQEKGHRGGTENLAAIAGFGAAAQFALAGLVTVETVRQLRDEF
                     EATVAALLPDAEFFGRGAARLANTTFFAIPGMKAETVQIAFDLAGVALSAGSACSSGR
                     VGPSHVLKAMGHENLGAVRVSIGPQTDAVDIEMFRSALAAIAARIASREKAA"
     gene            2512..3186
                     /locus_tag="VGM46_04525"
     CDS             2512..3186
                     /locus_tag="VGM46_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY4191888.1"
                     /translation="MPEVIFTGPAGRLEARYQPSKEKSAPIAIILHPHPQFGGTMNNK
                     IVYDLFYMFQKRNFTTLRFNFRGIGRSQGEFDHGTGELSDAAAALDWVQSLHPDSKSC
                     WVAGYSFGSWIGMQLLMRRPEIEGFISIAPQPNTYDFSFLAPCPSSGLIIHGDADKVA
                     PPKDVQGLVDKLHTQKGITITQKTLPGANHFFANDADLLLEECADYLDRRLAGELSDP
                     RPKRLR"
BASE COUNT          669 a         1104 c         1063 g          674 t
ORIGIN      
        1 gtcgaatatt tgatctcggc atcgtccaga gcgatcagtt cgaccacggc ggcgtgaagc
       61 tggttctcgt cgcgctgcgg ggcggtgcag ccttccagat aggaaacata ggcgccctct
      121 tcggcgatga tcagcgtgcg ctcgaattgg ccggtgccct tctcgttgat gcggaaatag
      181 gtcgaaagct ccatcgggca gcgaacgccc ttcggcacga acacgaacga gccgtcggtg
      241 aacaccgctg aattcaacgt ggcgtagtaa ttgtcggaga tcggcaccac ggaagcgaga
      301 tacttgcgca ccagttccgg gtgctcgcgg atggcctcgg agattgagca gaagatgacg
      361 ccggcggcgg ccagttcctt cttgaaggtg gtgacgaccg acacgctgtc gaacaccgca
      421 tcgacggcga cgcggcccga cttgtagaca ttgtcgttct cgccttcggc tgcatcgtcc
      481 tcgctggcag cagttggctt ctgcacgccg gccaggattt cctgctccct cagcggaatg
      541 ccgagcttgg cgtaggtttt cagcaattcg ggatcgactt cggccagcga agtcgggccg
      601 ggcgtcgatt tgggcgccgc atagtagaaa atatcctgga aatcgatctt cggataatgg
      661 acgcgcgccc aggtcggctc ctccagcgtc agccagcggc gataggcttc cagacgccat
      721 tcaagcatcc agtccggctc gtccttcttg gccgaaatga aacggatgat gtcttcgcta
      781 aggcccttgg gggccttgtc catcgcgatt tcggtttcga agccgtattt atattggtcg
      841 acgtcgatct tgcgaacccg atcgatcgtg tcctgcacag caggcatcag cgttctccat
      901 cctcgccggg ttcaaggccc ggcagttttc aaaccatggc accgccaaaa ggcgtcccct
      961 cggggagccc gcgaacgggc ggcgtaagtt ccatatagtg cgtcccgacc ggaaattcac
     1021 ccggccaaag cggaacacac ggcttgtcca gaccaaaacg gcccgatttc acattccgct
     1081 caggccgcct tttcgcggct ggcgatgcgg gcggcaattg ccgccagtgc cgagcggaac
     1141 atttcaatgt ccacggcatc ggtttgcggt ccgatggaca cgcgcaccgc acctagattt
     1201 tcatggccca tggccttcag cacatggctc ggcccgaccc tgccggacga acaggccgag
     1261 ccggcggaca gcgcgacgcc ggccagatcg aaggcgatct gcaccgtttc ggccttcatg
     1321 ccgggaatag caaagaatgt cgtattggca agccgggcgg ccccgcggcc gaagaattcg
     1381 gcatccggca acaatgcggc caccgtcgcc tcgaactcgt cgcgcaattg gcggaccgtc
     1441 tcgaccgtga ccaatccggc gagcgcgaac tgcgccgcag cgccgaaacc ggcgatcgcg
     1501 gcaaggttct ccgtgccgcc acgatggccc ttttcctgcc cgccgccggc gatcaggggt
     1561 gcgggcatca tcaggtcggc agccgcgacg atggcgccag cgccttgcgg tccgcccatc
     1621 ttgtgcgcgg agagaatcag acaatcggcg taacccgctg caatatcgag cggaatgcgg
     1681 cccgccgcct gtgcggcatc cagcaccagc acgccgccgg ccgccttgac gatggccgaa
     1741 atcttctcga ccggctggat aacgccggtc tcgttattgg cggcatggat ggctaccagc
     1801 ggcaggccat tcgccttgtc atggctggcc agcgcccccg ccaattcgtc caaccgaacg
     1861 atgccttcgc catcgacgcc gatccgcgac agctgttcgg caggaaagcg gccgccattc
     1921 agcaagcagg ggtgatcagc ctcgcagaca tagagatgac ccatgcgaag ggcagcgcgc
     1981 cccatccgcc agtccggcgt cagcagcatc gatgccgctt ccgtggcgcc ggaggtgaaa
     2041 acgacatggt ccggcctgcc gttgaccaag gcggcgacgc tgcgccgcgc ggcctcgatc
     2101 atctggcgcg cggcgcggcc ctcggcatgg acggaagatg gattggcgga tgcatcgagc
     2161 gcggcaagca tggcctggcg cgccacggga agcagcggcg cgctggcatt atagtcgaga
     2221 taggcgcgcc ttgccgccat tttcacttaa tcaatcctgt cagagactgc ctggcggtcc
     2281 gttagaaggc cattcagcga ggagagcgcg ttatttcctt gaaattccaa ggcgagccgt
     2341 cctatttacg catcgcgaca tgaatagctg gatttaatgg ttcggctatt cagttttgaa
     2401 caattctaaa ctagctttta agaagcccct cgcccttcgt caaggccgga atagggccag
     2461 agacagagtt gttccgggcg tcgttacctt cgattgcagt tggagtattt tatgcctgag
     2521 gtcattttta ccggtccggc cggccgcctg gaagcgcgct atcagccttc caaggaaaag
     2581 agcgccccga tcgccatcat cctgcacccg catccgcaat tcggcggcac gatgaacaac
     2641 aagatcgtct atgacctctt ctacatgttc cagaagcgca acttcaccac gcttcgcttc
     2701 aattttcgcg gcatcggccg cagccagggc gaattcgacc atggcaccgg cgagctttcg
     2761 gatgcggcgg cggcgctgga ctgggtgcag tcgctgcatc ccgattccaa gagctgctgg
     2821 gttgccggct attctttcgg ctcgtggatc ggcatgcagc ttttgatgcg ccggcccgag
     2881 atcgaaggtt tcatctcgat tgcgccgcag cccaatacct acgatttctc gttcctggcc
     2941 ccctgcccgt cttccgggct gatcatccat ggcgatgccg acaaggttgc gccgccgaag
     3001 gacgttcagg gtctcgtcga caagctgcat acgcaaaagg gcatcaccat cacgcaaaag
     3061 acgctgcccg gcgccaacca tttcttcgcc aacgacgccg atcttctgct tgaggaatgc
     3121 gccgactatc tcgaccggcg gcttgccggc gaattgtccg atccgcgccc gaagcggctg
     3181 cgctgaccga cgcagccggg agaagccggc tggctggcgc aacggttttt tgcgctgaca
     3241 cggtttcacg agggcaagtg gaagccctcc cgtgtcgccc cgcgcgggcg tttgcgaacg
     3301 gctggaagca ggcgcgcaac agccgatacg cccgaaatga cccggccggt ccggtctttt
     3361 gatggtccag acagcagatg atcgcctcta ttcagggcga tggccggcgt ttgaaggtgt
     3421 ggccggcgac ggccgctttt gccgttcagg cagcccaaaa atccagaaag tatccctgcg
     3481 caccggcagg gcgttgggcc tttggacaaa
//
LOCUS       DASYCS010000134         7271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_753763,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000134 DASYCS010000000
VERSION     DASYCS010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 7271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7271
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753763"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="VGM46_04530"
     CDS             <1..904
                     /locus_tag="VGM46_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HEY4191889.1"
                     /translation="GAIEATLDREQGSNAWLTLALREGKNREVRNILGSLGLDVTRLI
                     RISYGPFQLDDLKEGHVLEIKGRVLRDQLGERLVEESGANFDADITKPFSNRPVRRTE
                     PVFEEPQRAKPAREGDRRPIGEGGLIKNRKRREGSRDEALGKLSTKPEGGFAERGPRP
                     ERSERPERSERSARPERSERPDRPFGERGAKPERGGFGDKPKRGGKSEREQRPIEPPG
                     QRKANVWMAPGARPIGKGRAEAEKTQAEAKKARKSAIYGKPAGGKPFGKRDGAERPAG
                     KGDDRPRGGPKGPARGGSSADRRR"
     gene            885..1439
                     /gene="rsmD"
                     /locus_tag="VGM46_04535"
     CDS             885..1439
                     /gene="rsmD"
                     /locus_tag="VGM46_04535"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019857483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEY4191890.1"
                     /translation="MRIVGGEFRGRPLATPRTSSIRPTTDRTREAVFNVLAHRFADKL
                     DGARVLDLFAGTGALGLEALSRGASYGVFIEESSEGRGLIRTNVEAFGLTGRTKIFRR
                     DATALGEAGTISPFDLVFADPPYGRGLGERALQSAKAGGWLGGGALCVVEEAAASPFE
                     PGPGFVLLDERSYGETVIRFMQVA"
     gene            1536..2906
                     /locus_tag="VGM46_04540"
     CDS             1536..2906
                     /locus_tag="VGM46_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY4191891.1"
                     /translation="MTFHGDWLRLPVMAAVVAFSFAGPALAQAAPDGWKVTDFLLDNG
                     MEVVVIPDHRAPIVTHMVWYKVGSADEPPGKSGIAHFFEHLMFKATTNHKAGEFDAAI
                     AAIGGSNNAFTSYDYTAFHETVTPQALGQMMGFEADRMRNLILNDEVIATERDVILEE
                     RRSRIDNSPDALLDEEVNATLYQNQPYRVPVIGWKQEMEQLNRTDAKAFYDHYYTPNN
                     AVLVVAGDVEPETVKKLAEETYGKLARGPDLPPRIRPEEPEQNTQRTVTLRDARVSVP
                     SFSTQWVVPSYHTEKHGEAEALDLLAEILGGGTRSRLYQQLVVKQGIAASAGASFQGS
                     MLDATSFVVYGAPRGDAKIADVEAAVDAEVARIARDGVTADELDKAKKRFVRSTIFAR
                     DKQASMANMYGVTLATGGTVQDVEQWPDRIRAVTADEVKAVAARYLDIGRSTTGYLLP
                     QAQAGN"
     gene            2908..4266
                     /locus_tag="VGM46_04545"
     CDS             2908..4266
                     /locus_tag="VGM46_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY4191892.1"
                     /translation="MMLQLKILPARLAMPMATFLLSVVFLVLPAITARAAVTIQEVKS
                     DSGVSAWLVEDYSVPIVAIQFAFEGGSSQDPAGKEGLANLMTGLFDEGAGDLDSDTFQ
                     TRLDDAGAEMSFNATRDGIYGSMRMLAETKDEAFDLLRLAVEKPRFDQAPIDRIRGQV
                     LSGIIAGERDPNTVAAAKWAQALYPGHPYSRQDEGTKESIAAITADDLRAFHKANFAR
                     DGLHVAVVGAIDAKTLKIQLDRLFGGLPEKQALTAVPDTDPVLGQQMQVTYDQPQTSL
                     QLAYPGVKRSASDFYAAALMNEILGGGTFTSRLFKEVREKRGLAYGVSSALADSEHSA
                     ALVIGTATRSDRAAQTLAVVQDVVKKLADEGPTEQELAQTKQYLLGAYAINNLDSSGS
                     IASTLIQLQLDHLGIDYIQNREAYLNAVTLDQVKAVAKKLLSAKPAIMVVGPPLADGG
                     KG"
     gene            4263..5141
                     /locus_tag="VGM46_04550"
     CDS             4263..5141
                     /locus_tag="VGM46_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006332229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY4191893.1"
                     /translation="MSPTFGIAFGGGGARGLAHIHVIEALDELGIRPVAVAGSSIGAI
                     MGAGMAAGISGKDIHEHSRSILTRRAQVATRMWRARPGTFAEMMEGGFRVSQFNIERI
                     LKAFLPDDVPETFAELKIPLKVTATDFFGHQLAVLSEGKLHSALAASAAIPAVFRPVM
                     RDGRMLIDGGIYNPVPFDLIEHDADIVIAVDVVGAPTDAERKNPTTVDLMFGATQLMM
                     QSIITNKLKRCRPDILIRPPVSRFRVLDFMKVDAVMAETASVKDEVKRAIEAAVEARL
                     GHSGPKAAKRPKPAAK"
     gene            5229..6914
                     /locus_tag="VGM46_04555"
     CDS             5229..6914
                     /locus_tag="VGM46_04555"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEY4191894.1"
                     /translation="MSTHPAAPDTIRIAVAQLNPTVGDIAGNLAKAREARADAARQRA
                     DIVLFTELFISGYPPEDLVLKPAFLKACERAVEDFAKDTADGGPGVIIGTPLKRKSGT
                     HNSIIVADGGKIIAERFKADLPNYGEFDEKRVFQAGPELPGPINFRGIRLGIPICEDI
                     WGDLGVCETLVESGAEILLVPNGSPYYRAKMDARQQVVIRQVIETGLPMIYANQLGGQ
                     DELIFDGASFAIQADKSLAFQMSQFEEAVDVSVWKRDAGGWVCSQGPMSKIPDGEEAD
                     YRACMLGFRDYVNKNGFKNVVLGLSGGIDSAICAALAVDALGEERVQAVMMPYRYTSK
                     DSLKDAEDCARALGCRYDIIAIADPVDGFSQALAPLFAGTKPDITEENLQSRARGTIL
                     MAISNKFGSMVVTTGNKSEMSVGYATLYGDMNGGFNPIKDLYKMQVYALSRWRNAHVP
                     SGALGPGGEVIPVNIIDKAPSAELRENQTDQDSLPPYPVLDDILECLVENEMGVDDIV
                     ARGHDRVTVTRVEHLLYVAEYKRRQAAPGVKITKKNFGRDRRYPITNRFRDRG"
     gene            7062..>7271
                     /locus_tag="VGM46_04560"
     CDS             7062..>7271
                     /locus_tag="VGM46_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HEY4191895.1"
                     /translation="MTVTVRFAPSPTGRIHIGNARTALFNWLFAQKHRGRFIQRFDDT
                     DLARSRQEYADSILYDLHWLGIFQDA"
BASE COUNT         1383 a         2249 c         2333 g         1306 t
ORIGIN      
        1 tggcgcgatc gaggcgacgc tggaccgcga gcagggatcg aatgcctggc tgacgctggc
       61 gctgcgtgaa ggcaagaacc gcgaggtgag gaatattctc ggctctctcg gcctcgacgt
      121 cacccggctt atccgcattt cgtacggccc gttccaactc gatgacctca aggaaggtca
      181 tgtgctggag atcaaggggc gtgtcctgcg cgaccagctt ggcgagcggc tggtcgaaga
      241 atccggcgcg aatttcgatg ccgacatcac caagcctttt tcgaacaggc cggtgcggcg
      301 caccgagccg gttttcgagg agccgcagcg cgcaaagcct gctcgtgaag gcgaccgtcg
      361 ccccatcggc gaaggcggat tgatcaagaa tcgaaagcgg cgcgaaggca gccgcgacga
      421 ggcgctgggc aagctttcga ccaagcctga aggcggcttt gcggaacgtg ggccaaggcc
      481 cgaacgaagc gagcgccccg aacgaagtga acgaagcgcg cggccagagc gcagtgaaag
      541 gccggatcgg ccctttggcg agcgcggagc caagcccgaa cgcggcggct tcggcgacaa
      601 acccaagcgt ggcggcaaga gcgagcgcga gcagcggccg atagagccgc cggggcagcg
      661 caaggccaat gtgtggatgg cgccaggcgc acggccgata ggcaagggca gggcggaagc
      721 cgaaaagacg caggccgagg ccaagaaggc gcgcaagtcg gcgatttatg gcaagcccgc
      781 cggcggcaag ccgttcggca aacgcgacgg tgcggaacgt cccgcaggca agggcgacga
      841 ccgcccgcgt ggcggaccga agggaccggc gcgaggggga agcagtgcgg atcgtcggcg
      901 gtgagtttcg cgggcgtccg ctggcgacgc cccgcacctc gtcgatccgc ccgaccacag
      961 accgcacgcg cgaggcggtg ttcaatgtcc ttgcgcatcg ctttgcagac aagctcgacg
     1021 gcgcgcgcgt gctggatttg tttgccggca ccggcgcgct ggggctggag gcgctgtcgc
     1081 gcggcgcttc gtatggcgtg ttcatcgagg aatcgtcgga gggccgcggc ctcatccgca
     1141 ccaatgtcga ggcgttcggc ctgacgggac gcaccaagat ttttcgccgc gatgcaaccg
     1201 cgctgggcga ggcgggcaca atctctccct tcgatcttgt gttcgccgac ccaccctatg
     1261 gcagggggct tggcgaacgc gccttgcaat cagcgaaggc cggcggctgg cttggcgggg
     1321 gcgcgctttg cgtggtcgag gaagcggctg cttcgccgtt cgaaccgggg ccgggctttg
     1381 tgcttctcga cgagcgaagc tatggcgaaa ccgtgatccg cttcatgcag gtggcctgac
     1441 ttcggccttc tttaccgcgc aggtgacaaa caattcactt ttcgcgtggc aagaccctgc
     1501 ctatcgtccc cccgcatgaa cgcttcggag tttcgatgac atttcacggc gattggctgc
     1561 gtcttcctgt catggctgct gtcgtggcat tttctttcgc ggggccggct ttggcgcaag
     1621 ccgcgccgga tggctggaag gtgaccgatt tcctgctcga taacggcatg gaagtggtgg
     1681 tcattcccga tcatcgcgcc cccatcgtca cccatatggt ctggtacaag gtcggcagcg
     1741 ccgacgagcc gccgggaaaa tcgggcatcg cccatttctt cgagcatttg atgttcaagg
     1801 cgacgaccaa ccacaaggcc ggcgagttcg acgccgccat tgccgccatc ggcggttcca
     1861 acaacgcctt cacttcctat gattacaccg cctttcacga gacggtgacg ccgcaggcgc
     1921 tcggccagat gatgggtttc gaggccgacc ggatgcgaaa cctcattctc aacgacgagg
     1981 tgatcgccac cgagcgtgac gtcatcctgg aggaacgccg ctcgcgcatc gacaacagcc
     2041 cggatgcgct gctggatgaa gaggtcaacg ccacgctcta ccagaaccag ccctaccgtg
     2101 ttccggtgat cggctggaag caggaaatgg aacagttgaa ccgcaccgac gccaaggcgt
     2161 tctacgatca ctactatacg cccaacaatg cggtgctggt ggtggctggc gatgtcgaac
     2221 cagagacggt gaagaaattg gccgaggaaa cctacggcaa gctggcgcgc ggccccgatc
     2281 tgccgcctcg catccgtccg gaggagccgg agcagaacac gcagcgcacc gtgacattgc
     2341 gggatgcccg cgtcagcgtt cccagttttt ctacccaatg ggtcgtgccc tcgtaccata
     2401 ccgaaaagca cggcgaggcc gaggcgctcg accttctggc cgaaattctg ggcggcggca
     2461 cgcgcagccg gctctaccag caactggtgg tcaagcaggg cattgccgcc agcgccggcg
     2521 cctcgttcca gggttcgatg ctggatgcca ccagtttcgt cgtctatggc gcaccacgag
     2581 gcgatgcaaa gatcgcggat gtggaggcgg cggtggatgc cgaagtagcc cgcatcgcca
     2641 gggacggcgt gactgccgac gaactggaca aggccaagaa gcggttcgtg cgctcgacca
     2701 tcttcgcgcg cgacaagcag gcatcgatgg caaatatgta tggcgtgacg ctggcgacgg
     2761 gcggcaccgt acaggatgtc gagcaatggc cggaccgcat tcgcgccgtc accgccgacg
     2821 aggtcaaggc cgtggccgcg cggtacctcg acatcggcag gtcgaccacc ggctatctgc
     2881 tgccgcaagc gcaggcgggg aattgagatg atgcttcaac tcaagatact gcccgcgcgc
     2941 ctggccatgc cgatggcgac attcctgctt tccgtggttt tcctggtgct gccggccata
     3001 acggcgcgcg ccgccgtgac catccaggag gtcaaatccg attccggcgt ttcggcctgg
     3061 ctggtcgagg attattcggt gccgatcgtc gccattcagt tcgctttcga gggcggcagt
     3121 tcgcaggacc ccgccggcaa ggaagggctg gccaatctga tgaccggcct gttcgacgaa
     3181 ggtgccggcg atctggacag cgacacgttc cagaccaggc tggacgatgc gggcgccgag
     3241 atgagcttca acgccacgcg agacggcatc tacggttcga tgcggatgtt ggcggaaacg
     3301 aaggacgagg cgttcgacct gctcaggctc gccgtcgaaa agccgcgctt cgaccaggcg
     3361 ccgatagacc gcatccgagg gcaggtgctt tccggcataa tcgcggggga gcgcgacccc
     3421 aacacggtgg cggctgccaa atgggcgcag gcgctttatc cggggcaccc ttattcccgg
     3481 caggacgagg gaaccaagga aagcattgct gccattacgg cggacgatct gcgcgccttc
     3541 cacaaggcga attttgcccg cgacggactg catgtcgcag tggttggcgc catcgatgca
     3601 aagaccttga aaatccagct tgaccggctg tttggcgggc ttcccgaaaa gcaggccctg
     3661 acggccgttc ccgacaccga cccggtgctt ggccagcaga tgcaggtcac gtacgaccag
     3721 ccgcagacat cgctgcaatt ggcctatccg ggcgtgaagc gttcggcgtc cgatttttac
     3781 gcggcagccc tgatgaacga aattctcggt ggcggcacgt ttacttcccg cctgttcaag
     3841 gaagtccggg aaaagcgcgg gcttgcctac ggcgtcagtt ccgcgctggc cgacagcgag
     3901 cattcggcgg cgttggtcat cggcaccgcg acgcgctccg accgcgcggc gcaaacgctg
     3961 gccgtggtgc aggacgtcgt aaaaaaattg gccgatgagg ggccgaccga acaggaactg
     4021 gcgcagacca agcaatatct gctcggcgct tatgccatca acaatctgga ttcttccggc
     4081 tccattgcca gcacgctgat ccagcttcaa cttgaccatc tgggcatcga ctatatccag
     4141 aaccgcgaag cctatctcaa tgcggtcacc ctcgatcagg tcaaggctgt ggccaaaaag
     4201 ctcctgtcgg caaaacctgc gatcatggtc gtcgggccgc cgctggccga tggaggcaag
     4261 gggtgagccc caccttcggc atcgccttcg gcggcggtgg cgcgcgtggc ctggcgcata
     4321 tccatgtcat tgaagcgctg gatgaactcg gcatcaggcc tgtggcggtc gccggctcgt
     4381 cgatcggcgc gatcatgggc gcgggcatgg ccgccgggat ttccggcaag gatattcacg
     4441 agcattcacg ctcgatcctt acccgtcgcg cgcaagtggc gactcgcatg tggcgggcgc
     4501 ggcccggcac atttgcggag atgatggagg gcgggtttcg cgtcagccag ttcaacatcg
     4561 agcgcatcct gaaggctttc ctgccggacg atgtgcccga aacattcgcc gaactgaaaa
     4621 ttccgcttaa agtgacggcg acggatttct tcggccatca gcttgccgtc ctttccgaag
     4681 gaaagttgca ttcggcgctg gccgcttccg cggcgatccc ggcggtgttt cgtccggtca
     4741 tgcgtgacgg ccgcatgctg atcgacggcg gcatctacaa tcccgtgccg tttgacctga
     4801 tcgaacacga tgccgatatc gtcatcgccg tcgatgttgt cggcgcgcca accgatgccg
     4861 agcggaagaa cccgacaacg gtagatctga tgtttggcgc gacgcaattg atgatgcagt
     4921 ccatcatcac caacaagctg aagcgctgcc gccccgacat tttgattcgt ccgccggttt
     4981 cgcgctttcg cgtgctcgat ttcatgaagg tcgatgcggt gatggccgag acggcatcgg
     5041 tgaaggacga ggtcaagcgc gccatcgaag ccgccgtgga ggccaggctt ggccatagcg
     5101 ggccgaaggc tgcgaagcgt cccaagcctg ctgcaaaata ggtttcgccg accggcggcc
     5161 aaccgctccg gtcgcgtcgg ccacgaagcg cattgcccgc gcggctgggg cgcgatatta
     5221 ggcgtttcat gagcacgcat cccgccgccc ccgatacgat ccgcatcgct gtcgcccagc
     5281 tcaaccctac ggttggcgac attgccggca accttgccaa ggcgcgtgag gcgagagccg
     5341 atgccgcccg ccagcgcgcc gatatcgtgc tgttcaccga actgttcatt tccggctatc
     5401 cgcccgagga cctggttttg aagccggctt tcctgaaggc ctgcgaacgc gccgtcgagg
     5461 atttcgccaa ggacaccgcc gatggcggtc cgggcgtcat cattggcacg ccgctgaagc
     5521 gcaagagcgg cacccataat tccatcatcg tcgccgatgg cggcaagatc atcgccgaac
     5581 gcttcaaggc cgacttgccg aactacggcg agtttgacga gaagcgcgtt ttccaggcag
     5641 ggccggaact gcccggtccg atcaattttc gcggcataag gctaggcatc ccgatctgcg
     5701 aggacatctg gggcgatctc ggcgtctgcg agacgctggt cgaatcgggc gctgaaatcc
     5761 tgctcgtgcc gaacggctcg ccctattacc gcgccaaaat ggatgcgcgc cagcaggtcg
     5821 tcatccggca ggtgatcgag acgggcctgc ccatgatcta cgccaaccag cttggcggac
     5881 aggacgaact tattttcgac ggtgcttctt tcgccatcca ggccgacaag tcgctggcct
     5941 tccagatgag ccagttcgaa gaggccgtcg atgtatcggt atggaagcgc gatgccggcg
     6001 gctgggtctg ctcgcaaggg ccgatgtcca aaatcccgga cggggaggag gccgactacc
     6061 gcgcctgcat gttgggcttc cgcgactacg tcaacaaaaa cggcttcaag aatgtcgtgc
     6121 ttggcctttc cggcggcatt gattcggcga tttgcgcggc tcttgccgtc gatgcgcttg
     6181 gtgaggaacg cgtgcaggcg gtgatgatgc cttatcgcta cacgtcgaag gattcgctga
     6241 aagacgccga ggattgcgcc cgcgcgctcg gttgccgcta cgatatcatc gccatcgctg
     6301 accccgtcga tggattctcg caggcattgg ccccgctgtt tgccggcacc aagccggata
     6361 tcaccgagga aaatctccag tcgcgcgctc gtggcaccat cctgatggcg atttccaaca
     6421 agttcggctc gatggtggtg acgaccggca acaagtcgga aatgtcggtc ggctatgcga
     6481 cgctctatgg cgacatgaat ggcggcttca atccgatcaa ggacctctac aagatgcagg
     6541 tctatgcgct gtcgcgctgg cgcaacgcgc atgtgccttc cggcgcgctc ggtccgggcg
     6601 gcgaggtcat cccggtcaac atcatcgaca aggcgccctc ggccgagctg cgcgagaacc
     6661 agaccgatca ggattcgctg ccgccctatc cggtgctgga cgacattctg gaatgcctcg
     6721 tcgaaaacga gatgggcgtc gatgatatcg ttgcccgtgg ccacgaccgt gtcaccgtca
     6781 ccagggtgga acatctgctc tatgtggcgg aatacaagcg ccgtcaggct gcgcccggcg
     6841 tgaagatcac caagaagaac ttcggccgcg accgccgcta tcccattacc aaccggttcc
     6901 gcgacagagg gtagggcgag ccgagaggct gcttcattta aggtgccagc atttccgaac
     6961 aggccgcgag atgcggctgg accgccgctg attggatagc cagcgcatcg cagcgccgcc
     7021 gaagtcttgt caacgcggac cgcatccgct acgccctcgc catgacagtc accgttcgtt
     7081 tcgcgccgtc gcccaccggc cgcattcata tcggcaatgc gcgcaccgcc ctgttcaatt
     7141 ggcttttcgc gcaaaagcac aggggccgtt tcatccagcg cttcgacgat accgacctgg
     7201 cgcgttccag gcaggaatat gccgattcga tcctctacga tcttcactgg ctggggattt
     7261 ttcaggacgc t
//
LOCUS       DASYCS010000135         2288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1187569,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000135 DASYCS010000000
VERSION     DASYCS010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1187569"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VGM46_04565"
     CDS             <1..546
                     /locus_tag="VGM46_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019857236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEY4191896.1"
                     /translation="VRAYANDMLLGEAVASPDGHFLVEAKRDSPVGQYNVRVDALDAD
                     GVKVAARATVPFEREPGEAVAAVAADATKPATAQTDTAAAEPSASTATQTPETSAPKL
                     QHADGAVIIRRGDSLWRISRRVYGHGTRYSTIYLANQKQISDPDRIWPGQVFAVPDKS
                     KEGEPADLKSLGEQATTTPAQ"
     gene            605..937
                     /locus_tag="VGM46_04570"
     CDS             605..937
                     /locus_tag="VGM46_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY4191897.1"
                     /translation="MDDLQTANRMKCASPDSRAMAAKLAALSHPTRLEILKHLSARRS
                     CCCGEVVERMDLAQSTVSQHLKILVAAGLVRFAADRQRSRYEVDRKALAAISTAVAEL
                     VDSCCSGS"
     gene            958..>2288
                     /locus_tag="VGM46_04575"
     CDS             958..>2288
                     /locus_tag="VGM46_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006331355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEY4191898.1"
                     /translation="MAEKTVSSDSGSTLKTLRHLWPYMYPADRADLRWRVTWATLLLV
                     AAKLVLVAGPYFFKWATNALAGEGKTPPLPEFLLAPIVLVVAYNFMRLVQLGFNQLRD
                     ALFARVGQYAVRQLAYRTFIHMHELSLRFHLERRTGGLSRIIDRGTKGIETIVRFTML
                     ATAPTIIEFALTAIIFAVAYGWIYVAVVAATVCLYTLYTVKASDWRIAIRREMNDSDT
                     DASTKAVDSLLNYETVKYFGNERMEAERYDRSMARYEIAATKTWTSLGWLNFGQGVIF
                     GAGMTVVMVLSAREVQAGTQTVGDFVFVNALLMQLSVPLNFIGQIYREIRQGLTDIEN
                     MFDLLGVEREVCDEPDAAPLVVGPGKVEFRDVHFSYDPNRNILKGVSFEVPAGRTVAI
                     VGPSGAGKSTISRLLFRFYDVQEGAVLIDGQDIRHVTQESLRAAIGMVPQDT"
BASE COUNT          409 a          746 c          704 g          429 t
ORIGIN      
        1 gtgcgcgcct acgccaacga catgcttctg ggcgaggcgg tggcctcgcc tgacggccat
       61 ttcctggtcg aggcgaagcg cgatagtccc gtcggccagt acaacgtccg cgtcgatgcg
      121 ctggatgccg atggcgtcaa ggtcgcggcg cgcgccaccg tgccgttcga gcgcgagccg
      181 ggcgaggcgg tcgcggcagt cgctgccgat gccaccaagc cggcgacggc acagacggac
      241 accgccgcgg ccgaaccctc cgcaagcacg gcaacgcaaa ctcctgaaac ctccgccccc
      301 aagcttcagc atgccgacgg cgcggttatc atccggcgcg gcgattcgct gtggcgcatt
      361 tcgcggcgcg tctacgggca tggcacccgc tattcgacca tttatctggc caaccagaag
      421 cagatcagcg atcccgaccg catctggccg ggccaggtct tcgcggtgcc ggataagtcg
      481 aaggagggcg aacctgccga cctgaagtcg ttgggtgaac aggcgaccac cacaccggcg
      541 caatagtttt tcacgaatga gtttacttgc gtaaaaccat tccatcgtct atcgccgatg
      601 aacgatggat gatctgcaaa ccgcaaaccg catgaaatgc gcgtcgcccg acagccgggc
      661 gatggcggcg aagcttgccg cgctgtccca tccaacccgc ctcgaaatcc tcaaacatct
      721 ctcggcacgc cgttcctgct gttgcggcga ggtggtcgag cgtatggatc tggcgcaatc
      781 caccgtctcg cagcacctga aaatcctggt tgcggccggc cttgtccgct tcgccgccga
      841 ccggcagcgt tctcgctacg aggttgatcg caaagcgctt gccgccatct ccacggcggt
      901 cgcggaactc gtcgattcct gctgttccgg cagctgaacc catacaaggc aataaaagtg
      961 gctgaaaaga ccgtctcgtc cgattccggc tcgaccttga aaacgttgcg ccatctctgg
     1021 ccatatatgt atccggccga ccgcgccgac ctgcgctggc gggtcacctg ggccacgctg
     1081 ttgctggtcg ccgccaagct tgtgttggtg gccgggccgt atttcttcaa atgggcgacc
     1141 aatgcgctgg cgggcgaggg aaagacgccg cccttgccgg aattcctgct cgcgccgatc
     1201 gtgctggtcg tcgcctataa tttcatgcgc ctggtgcaac tgggcttcaa ccagcttcgc
     1261 gatgcgctgt ttgcgcgcgt cggccagtat gccgtgcgcc agcttgccta ccgcaccttc
     1321 atccacatgc acgaattgtc gctgcgcttt catctggagc gccgcaccgg cggcctgtcg
     1381 cgcataatcg accgcggcac caagggcatc gagaccatcg tgcgcttcac catgctggcg
     1441 accgcgccga ccatcatcga gttcgcgctg accgccatta tcttcgccgt cgcctatggc
     1501 tggatttatg tggcggtggt cgccgcgacg gtctgcctct acacattata cacggtcaag
     1561 gcgagcgact ggcgcatcgc catccgccgc gagatgaacg acagcgatac cgacgccagc
     1621 accaaggcgg tcgattcgtt gctgaactac gaaaccgtca aatatttcgg caatgagcgc
     1681 atggaggccg agcgctatga ccgctccatg gcgcgctacg agatcgccgc gacgaagacg
     1741 tggacgtcgc tcggttggct gaatttcggg cagggagtga tctttggcgc cggcatgacc
     1801 gtggtgatgg tgctgtcggc gcgcgaagtg caggccggca cgcagacggt cggcgatttc
     1861 gtcttcgtca acgcgctgct gatgcagctt tccgtgccgc tcaacttcat cggccagatc
     1921 taccgggaaa tccgccaggg gctgaccgac atcgagaata tgttcgacct gctcggcgtc
     1981 gagcgggagg tctgcgacga gccggatgcc gctccgctcg tcgtcggccc cggcaaggtc
     2041 gagttccgcg acgtgcattt ctcctacgac cccaaccgca atatcctcaa aggcgtcagt
     2101 ttcgaggtgc cggcaggcag gacggtcgcc atcgtcgggc cgtccggcgc gggcaagtcc
     2161 accatttcgc ggcttctgtt caggttctac gacgtgcagg aaggcgccgt gctgatcgac
     2221 gggcaggata tccgccatgt gacgcaggag agcctgcgcg ccgccatcgg catggtgccg
     2281 caggatac
//
LOCUS       DASYCS010000136         6517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4087913,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000136 DASYCS010000000
VERSION     DASYCS010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 6517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6517
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4087913"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VGM46_04580"
     CDS             complement(<1..396)
                     /locus_tag="VGM46_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006279860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY4191899.1"
                     /translation="MDTLTRMRAFIDVVEAEGFSAAARKIGRSKALLSKYVRELEDEL
                     GALLLNRTTRQFSMTEAGHTYYQRSSEIIREIDSLSDAVRESSGDVRGRIKLSAPRTF
                     ADAPIGQSLIDFARQYPEIVLDVRLDDRFV"
     gene            1055..2188
                     /locus_tag="VGM46_04585"
     CDS             1055..2188
                     /locus_tag="VGM46_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002966479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HEY4191900.1"
                     /translation="MATQRIMDFLATRRPEGPCLVLDLDVVADNFRAFERALPSSKIY
                     YAIKANPAPEILRLLAQMGSSFDAASVAEVEMAMNAGAPASRISFGNTIKKERDIARA
                     YQLGVRLFAVDCVEEVEKVARVASGARVFCRVLTDGEGAEWPLSRKFGCVPAMAINVL
                     RRAKELGLVAYGVSFHVGSQQVDLTAWDRALGDAKQVFATLAEEGIVLKMVNMGGGFP
                     TRYLRDVPAAEAYGHAIFEALSKHFGNAIPETIIEPGRGMVGNAGVIKSEVVLISKKA
                     DNDNLRWVFLDIGKFGGLAETMDEAIRYPLVTRRDGGETSPCVLAGPTCDSADVMYEK
                     TPYPLPLSLTIGDEVLIEGTGAYTTTYSAVAFNGFEPLRSYVI"
     gene            2384..2953
                     /locus_tag="VGM46_04590"
     CDS             2384..2953
                     /locus_tag="VGM46_04590"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEY4191901.1"
                     /translation="MPAEILNSAAAFEIMAEIAADILAREALLDRAMGPRRKRKSSEK
                     LRRGRRPSEGLAFVARTPSGAVAGTVRLWDVTLGEGGKSALLLGPLAVDPALKNAGLG
                     SALMRHAIAEAARLGHEIILLVGDAPYYGRFGFSAEKTGRLSMPGPYERHRFLALELV
                     PGALDGAHGTLKASGRRLSGKGERAAIAA"
     gene            complement(3055..3339)
                     /locus_tag="VGM46_04595"
     CDS             complement(3055..3339)
                     /locus_tag="VGM46_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEY4191902.1"
                     /translation="MGVQDIAEQIRSRVESGGFDHSVKFDTGTDGVILIDGAAISTTD
                     GPADCTIKLSLDDLESLIAGDLNPTMAFMTGKIKIEGDMTVAMALSQLIA"
     gene            complement(3474..3995)
                     /locus_tag="VGM46_04600"
     CDS             complement(3474..3995)
                     /locus_tag="VGM46_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191903.1"
                     /translation="MIKFIAAAIWICIATVGAVFYSFQSAGEHGEDAAPKPMLGGLDY
                     VNTDIISVPLVRNSAVQGYLLTKLVYTVEPAQLAKLSIPAEALITDQVYSYLYSNPQI
                     DFTKKETLDVDAFRNNIRDSINARVGEQLVHEVLIDNVDFLTKDDIRDNAIRRKKGAD
                     ETPAKEAKPAGEH"
     gene            complement(4003..4428)
                     /locus_tag="VGM46_04605"
     CDS             complement(4003..4428)
                     /locus_tag="VGM46_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006199420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191904.1"
                     /translation="MTDYSEFTSNLPMLANAPAGPVPVARIGNLDAIIGRIEEAVEEE
                     TAAIRTDTSFDLKASNARKSRCLYELNRAIKGMGQPEALEQHREGLTRLRQKLTRNEA
                     AILAHLNAVTEVANLLKNAIQRAEADGTYSAGEFGWAQS"
     gene            complement(4434..4988)
                     /locus_tag="VGM46_04610"
     CDS             complement(4434..4988)
                     /locus_tag="VGM46_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HEY4191905.1"
                     /translation="MAISPPSDIVLDVARAAGPADIEAARAALTKRAGGASAPFSVGE
                     APASTLSRTATQGTDGGDATKFKHFEAMVLQTFIQNMMPKDTEGVYGKGLAGDMWKSQ
                     MAERIADVMAERGGIGIAKSMLADHYMEGKRKVPIGQVSGGPEKTEIDHRTALSTSMV
                     QEAERKMARSMTGEEEPVKAGEKV"
     gene            complement(5086..5472)
                     /locus_tag="VGM46_04615"
     CDS             complement(5086..5472)
                     /locus_tag="VGM46_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018429293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191906.1"
                     /translation="MTTRRQRLKKLVLVQEQLKSLHETRHATFLSQAHAAEAEARELV
                     ESFDAQGSLSGLFPDLYHRRIANAVTRQEDNLEQARHEAGLIAAATARTNMVERAYEQ
                     VSRRDEREQSDRDRLDLIGQRRKTPE"
     gene            complement(5478..6230)
                     /gene="fliR"
                     /locus_tag="VGM46_04620"
     CDS             complement(5478..6230)
                     /gene="fliR"
                     /locus_tag="VGM46_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HEY4191907.1"
                     /translation="MPALSQTVVIAAFLAFCRVGACFMLMPGISSARVPLQVRLFVSV
                     AVTFGLLSFLWDKIYPYADARPAVLLPMIASELLIGGLIGAMTRLYMEALRFIGAAIA
                     MQIGLTGTGGPVIEESDPQAALAEIISLSALLFLFVFDFHHEVIRALVSSYTVAPVNV
                     FFNPQAALVDVTDTLSDSFFLVLRLGSPFVAYAILVNLTIGFVNKLTPQIPIYFISLP
                     FVIAGGMIIFYFAVGTLLSLFTDGFVDITLAR"
     gene            complement(6307..>6517)
                     /locus_tag="VGM46_04625"
     CDS             complement(6307..>6517)
                     /locus_tag="VGM46_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HEY4191908.1"
                     /translation="PRQLEEFGQDATKAIRKHLEAGERFVLVTAPDARPYVRMIIERL
                     FTTLPVLSHVEIAKGVEVRVLGTIS"
BASE COUNT         1171 a         1946 c         2110 g         1290 t
ORIGIN      
        1 gacgaagcgg tcgtcgaggc gaacgtcgag gacgatttcg gggtattgcc ttgcaaagtc
       61 gatcagcgac tggccgatcg gggcatcggc gaaggtgcgc ggcgccgaca gcttgattcg
      121 gccgcgcaca tcgcccgatg attcgcgcac ggcatccgaa aggctgtcga tttcgcggat
      181 gatctccgac gagcgctggt aataggtatg gccggcctcg gtcatcgaga actggcgcgt
      241 cgtgcgattg agcagcagcg cgcccaattc gtcttccagt tcacgcacat atttcgacag
      301 aagcgccttg gaccggccga tcttgcgggc ggcggcggaa aagccttccg cttccacaac
      361 gtcgataaag gcacgcatgc gggtgagggt gtccatggcg ttagcctttc ggtctgttgg
      421 acagggcaca tgggaaaaca ggccgggggc gggaccattt ttcagaaaaa tcagcaaggc
      481 cgtccgcagc ttataccgga cgagcctgcc tgttggaaga accagcggcg catgggcttt
      541 cgctcgccgt aaaattaaac gtgaaggctg aaattagtca ttgattggtc atgattctct
      601 gcctatatcg cgcttgccca aagtcgcacg tgcctgtggg tgtccgccgg tcctcagaat
      661 ggtgagggaa atcggtaagg taactggagc caacccctcc agtcggttta gcggccaacc
      721 gccaaatcga ggacaccttg aagcaacgac ggtgcgggcc tttctggttc tcttccggtt
      781 gtccaaagat cggggttact caaagaggca caccttcatt gccggcagtg cggaacgggg
      841 ttctcccaat ccaagccaag gcagacaaag cgggtttctg gccgggcaag gccattaacc
      901 catcgccgcg agacggcgtt ccgtggttcc ggtgtctcca ccggctggtt ccatggattc
      961 ctgtcccgcc tttgtttgat ccgcgtgttc gtgaggccga ggcccgcgtt gcgcaccctt
     1021 gttcgcgcgt gagaacgcgc tttggagaga cccgatggca acccagcgca ttatggactt
     1081 cctcgccacc cgacgaccgg aaggcccctg cctcgtgctc gacctcgacg tcgtggccga
     1141 caatttccgc gccttcgagc gcgcgctgcc cagctcgaaa atctactatg cgatcaaggc
     1201 caacccggcg ccggaaatcc tgcgcctgct ggcgcagatg ggatcgtcct tcgacgcggc
     1261 ctcggtggcc gaggtcgaaa tggcgatgaa tgccggcgcg ccggccagcc gcatttcgtt
     1321 tggcaacact atcaagaagg agcgcgacat tgcgcgcgcc taccagctcg gcgttcggct
     1381 gttcgcggtc gattgcgtcg aggaagtgga gaaggtcgcc cgcgtcgctt ccggcgcgcg
     1441 cgtgttctgc cgcgttttga ccgatggcga aggcgcggaa tggccgctgt cgcgcaagtt
     1501 cggctgcgtt ccggcgatgg cgattaacgt gctgcgccgc gccaaggaac tgggcctcgt
     1561 cgcctatggc gtgtcgttcc atgtcggctc gcagcaggtc gacctcactg cgtgggaccg
     1621 cgcgctgggc gacgccaagc aggttttcgc cacgctggcc gaagaaggca tcgtcttgaa
     1681 gatggtcaat atgggcggcg gcttcccgac gcgctatctg cgcgacgttc cggcggcgga
     1741 agcctatggc cacgccattt tcgaggcgct gagcaagcat ttcggcaatg ccattcctga
     1801 aaccatcatc gagccgggcc gtggcatggt cggcaatgcc ggcgtgatca agtcggaagt
     1861 cgtcctgatt tccaagaagg ccgacaacga caatctgcgc tgggtgttcc tcgacatcgg
     1921 caagttcggc ggtctcgccg agacgatgga cgaggcgatc cgctatccgc tggtcacccg
     1981 gcgcgacggc ggcgaaacct cgccttgcgt gctcgccggc ccgacctgcg attcggccga
     2041 cgtgatgtac gagaagacgc cgtatccgct gccactgtcg ctgaccatcg gcgacgaggt
     2101 gctgatcgag ggcaccggcg cttatacgac cacctactcg gcggtcgcgt tcaacggctt
     2161 tgagccgctg cgatcctacg tgatctgata attcgggcag aagggccggg aggcctgtct
     2221 gcaaagggta atttctgagc ttccggagct cacgtaccat aggtacgctc cgctccggtt
     2281 ctcgaaatcg ccattttcgc caaggcctgc cagccttctg tcccgaattc acccactgga
     2341 actgttcgct tgtggaaaaa tccactcgtg aaggattacg gtcatgcccg ctgaaatctt
     2401 gaattccgcc gctgctttcg aaatcatggc tgaaatcgcc gccgacattc tcgcgcgtga
     2461 agccctgctt gaccgcgcca tgggtccgcg gcgcaagcgc aaatcgtcgg agaagctgcg
     2521 gcgcgggcgt cgtccgtccg agggacttgc cttcgtcgcg cgtacgccct cgggcgcggt
     2581 ggcgggcacc gtgcgcctgt gggacgtgac gttgggcgag ggtggcaagt ctgcccttct
     2641 gcttgggccg ctggcggtcg atcccgcgtt gaagaatgcc ggtcttggct cggctctgat
     2701 gcgccacgcc atagccgagg ctgcccgcct cggtcatgaa atcatcctgc tggtaggcga
     2761 tgcgccctat tacgggcgtt tcggcttttc ggctgaaaag accggccggc tttcgatgcc
     2821 tggcccgtat gagcgccacc ggttcctggc gcttgagctg gtgcccggtg cgctggacgg
     2881 cgcgcatggc acgctgaagg cgtccggccg ccggctgagc gggaaaggcg agcgcgcagc
     2941 aatcgccgca taaaacgcaa tctcgaagcc gcaaggcggc tgcgaacacc gcttggcaat
     3001 caggctgagc ctgttccgtt caatgcggcg gaaacaggtt tcggaccgga tgagtcaggc
     3061 gatcaattgg ctaagcgcca tggcgaccgt catgtcgcct tcgatcttta tcttgccggt
     3121 catgaacgcc atggtgggat tgagatcgcc ggcaatcagc gattccagat cgtccagcga
     3181 gagcttgatg gtgcagtcgg ccgggccatc ggtggtcgaa atggcagcgc cgtcgatgag
     3241 gatcacgccg tccgtgcccg tgtcgaattt cacggaatga tcgaagccgc cgctttcgac
     3301 gcgcgacctg atctgctcgg ctatgtcctg aacgcccatc gcgggtctcc ttgtccttgc
     3361 tttgcctgtg cggccagcca ggctggcgcg cttgatgggg acattacggg tccacaccgt
     3421 tatggctata gctgttaatt gacgtttacg tcaaccaaat gacggcttcg ccatcaatgt
     3481 tccccggccg gcttggcctc cttggcgggg gtttcgtcag cgcctttctt gcggcggatg
     3541 gcgttgtcgc gaatatcgtc cttggtcagg aagtcgacat tgtcgatcaa gacttcgtgc
     3601 accagttgct cgccgacgcg cgcgttgatc gaatcgcgaa tgttgttgcg gaacgcgtcg
     3661 acatccagcg tttccttctt ggtgaagtcg atctgcggat tggaatagag gtaggaatag
     3721 acctgatcgg taatcaacgc ctcggcgggg atcgacagct tggccaactg ggccggctcg
     3781 accgtgtaga cgagcttggt caggagataa ccctggacag ctgaattgcg caccagcggc
     3841 acggaaataa tgtcggtgtt gacgtagtcg aggccgccca gcatcggctt tggcgctgcg
     3901 tcctcgccat gttcgccggc agactggaac gaatagaaca ccgcgccgac ggtagcgatg
     3961 caaatccaga tcgctgcggc gatgaatttt atcatcggtc gttcacgact gcgcccagcc
     4021 gaattcgccg gcggaatagg tgccgtccgc ttccgcccgc tggatcgcgt tcttcagcag
     4081 attggcgact tcggtgaccg cgttgaggtg ggcgaggatg gccgcctcgt tgcgggtcag
     4141 cttctggcgc aggcgggtaa gcccttcgcg gtgctgttcc agcgcttccg gctgtcccat
     4201 gcccttgatg gcgcggttca gttcgtagag gcagcggctc ttgcgggcat tcgaagcctt
     4261 gaggtcgaag ctggtatcgg tgcggatggc ggcggtttct tcctcgaccg cctcctcgat
     4321 gcgcccgata atggcatcga gattgccgat gcgcgcgacg ggaaccgggc ctgccggcgc
     4381 gttggccagc atgggcaggt tggaggtgaa ttccgaatag tcggtcattg tgcctagacc
     4441 ttctcgcctg cttttacggg ttcttcttca ccggtcatgg agcgcgccat cttgcgttcc
     4501 gcttcctgga ccatggaggt ggagagcgcc gtccggtggt ctatctcggt cttttccggc
     4561 ccgccggaaa cctggccgat cggcaccttg cgcttgcctt ccatatagtg gtcggccagc
     4621 atcgacttgg cgatgccgat gccgccgcgc tcggccatca catcggcgat gcgttcggcc
     4681 atctgcgact tccacatgtc gccggccagg cccttgccgt aaacgccctc ggtgtccttc
     4741 ggcatcatgt tctggatgaa ggtttgcagc accatcgctt cgaagtgctt gaacttggtc
     4801 gcgtctccgc catccgtgcc ttgcgtggcc gtgcgcgaaa gcgtcgatgc cggcgcctcg
     4861 ccgacggaga agggcgcgga agcgcctccg gcgcgcttgg tcaaggctgc gcgcgccgcc
     4921 tcgatatcgg ccggtccagc cgcgcgggca acgtccagaa caatgtcgct tgggggagaa
     4981 atcgccaaga aaacctgcct tccaatgtct tttcgtcggc tggcatcatg ccaaaacaag
     5041 cttgcggcag gcttgccggc atggctgcgg cgagcgcctg ggtggctact ccggggtttt
     5101 gcgcctttgg ccgatgaggt ccagcctatc gcggtcggac tgctcgcgct cgtcacggcg
     5161 gcttacctgc tcgtaggctc gctccaccat gttggtgcgg gcggtggcgg cggcgatcaa
     5221 gccagcctca tggcgcgcct gttcgaggtt atcctcctgg cgcgtcaccg cattggcgat
     5281 acggcggtga tagagatcgg gaaacaggcc cgacagcgaa ccttgggcgt cgaagctttc
     5341 gaccagttcg cgggcttcgg cttcggcggc atgggcttgc gaaagaaacg tcgcgtggcg
     5401 ggtttcatgc agcgacttca attgctcctg caccaaaacc agtttcttca gccgctgcct
     5461 tcgcgtggtc atgcgcgcta cctggccaat gttatgtcga cgaagccgtc ggtgaacaat
     5521 gaaagcaagg tgccgacggc gaaatagaag atgatcattc cgcccgcgat gacgaagggc
     5581 agcgagatga agtagatcgg aatctggggc gtcagcttgt tgacgaagcc gatcgtcaga
     5641 ttgaccagga tggcataggc gacgaacggg ctgccgaggc gcagcaccag aaaaaacgaa
     5701 tccgacagcg tatcggtgac atcgacgagg gctgcctgcg ggttgaagaa aacgttgacc
     5761 ggcgcgaccg tgtaggaaga aaccagcgcg cggatcacct cgtggtggaa atcgaagacg
     5821 aacaggaaaa gcagcgccga aagcgagatg atttcggcga gtgccgcctg cggatccgat
     5881 tcctcgatga ccgggccgcc cgtgccggtc aggccgatct gcatggcaat cgccgcgccg
     5941 atgaagcgca gggcctccat gtagagcctc gtcatagcgc cgatcagccc gccgatcaaa
     6001 agctcggaag cgatcatcgg cagcaatacg gccgggcgcg catcggcata ggggtagatc
     6061 ttgtcccaca ggaagctgag caggccgaag gtgacggcga ccgacacgaa cagccggacc
     6121 tgcaacggca cgcgggcgct ggatattccg ggcatcagca tgaagcaggc gccaacccgg
     6181 cagaaggcca gaaacgccgc gatgacgacg gtttgcgaga gggcgggcag accggtcatg
     6241 tcatgcgact gcggggagcc gcgccgaggc ccgccgaaca gggggccgct atctggcgcg
     6301 cgccgctcac gagatcgtgc ccagcaccct gacctcgacg cccttggcga tttcgacatg
     6361 gctgagcacg ggcagtgtgg tgaacaggcg ctcgatgatc atgcgcacat agggccgcgc
     6421 gtctggcgcg gtgacgagga cgaaacgctc gccggcttcg agatgcttgc ggatggcctt
     6481 ggtggcgtcc tggccgaatt cctccagctg gcgcgga
//
LOCUS       DASYCS010000137         4709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4956897,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000137 DASYCS010000000
VERSION     DASYCS010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4709
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4956897"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1160
                     /locus_tag="VGM46_04630"
     CDS             <1..1160
                     /locus_tag="VGM46_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY4191909.1"
                     /translation="LDWNTLIANKDREIARLDAVHDKSVRAAGGEPVRSHATLVDNHT
                     IRLEAEGRTVTADQILIATGSRPAAHPVHPGQEHAITPNEAFDLTELPKAIVIEGDDY
                     IAVEFANIFHGLGVETTLVCPRAEILSGFDMGLRQELHEAMERKGIRVLCQATSKKVE
                     RQPDGRLAIVVNDDKALVADQLILAAGRRANTEMLGLDAAGVKTGKTGEIIVDDYSRT
                     NVENIWAIGDVTNRVQLTPVAIHEAMCFVETAFKGNPTAPDHGAIATAVFSQPEIGTV
                     GLSEGDAGKQFADLEIYRALFRPMRHTLSGNQEKMLIKLVVDGASRKVLGAHILGPDA
                     AEMAQVLAIPVKAGLTKGDFDRTMAAHPTAAEELVTMYKPSYRVKNGERAG"
     gene            complement(1189..2976)
                     /locus_tag="VGM46_04635"
     CDS             complement(1189..2976)
                     /locus_tag="VGM46_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEY4191910.1"
                     /translation="MKSDPADQRHLGDFTTDARVLLIAAIAVIVGTAALGAGILLIKL
                     IKLVTNLAYFGQFSLQDLKLADTPLGLWAVLVPVVGALVIGLMARYGSEKIRGHGIPE
                     ALEAILLGRSRLDAKVAILKPLSSAISIGTGGPFGAEGPIIMTGGAIGSLIAQALPVT
                     DNERKTLLVAGAAAGMTTVFGTPIAAIMLAVELLLFEWTPRSFIPVAVAAIIAEVERT
                     LLHLPSPLFAFNGGMETSFAALGGWVAIGIASGLLSGLLTQAVYACEDAFLKLPIHWM
                     WWPMIGGLIVGLGGLIEPNALSVGYDNIARMLNGETLAKAAILLLVVKTIIWSVALGS
                     GTSGGVLAPLLIIGGAMGVVLGGFLPVADPGFWALLAMAATMGGTMRAPLTATFFAVE
                     LTGNTHVLVPLITACAAAHAVTVLLMKRSILTEKIARRGLHLVREYRVDPFALTRVSE
                     VMTKNVQTVSSSMTLHGAAAYLTSPETHHPSFPVVDPDGRVLGIVDPPAILRWRRAGR
                     HRKTTLAELLGGTKITLAYPDEYLEGLADKLQTANVSHLPVVARADNRLVGYVGWKDL
                     MRVRMKTRAEEQDRTRFFGAAGRALPPAD"
     gene            3109..3546
                     /locus_tag="VGM46_04640"
     CDS             3109..3546
                     /locus_tag="VGM46_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEY4191911.1"
                     /translation="MSVEKSAGKQRSQITQADYEALSDFRYLLRRFLEFSEKAARDAG
                     LQPRQHQALLAIKGMGERGPVGVGDLAERLCIRHHSAVELVNRLGEAGLVSREHDKVD
                     QRRVILHLTERAEAYLAGLSAAHLDELSRIEPMLTRILSHSKG"
     gene            complement(3600..>4709)
                     /locus_tag="VGM46_04645"
     CDS             complement(3600..>4709)
                     /locus_tag="VGM46_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006333620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY4191912.1"
                     /translation="VDIDQTWARINVVNAGYDSFERIYNFVGARIVAYGPGLLPFISS
                     RVDLKGYITGAELVNSLISQCSWFIHVMPAIAALRANSTRVTDLAKAIENVQHPSEFY
                     KQTGSSDFTYVRQNPVFGLSVQNLELRQQGHDTVPFLSAERLRFKRGEWTFIKGESGC
                     GKTSLVKAINGLWSYGQGTIVFPEGVKSFYAAQEVKLPPLSLKELVCLPDTPACHTDP
                     QVASALHKAGLGDFIEHLDQESRKGKSWDQLLSGGQKQKLVVARILVQRPGLLFLDEA
                     TGALDPEGKIAFHQAIKDNCPNVTVISIMHEAVAPKSADGVDFYNSVLMIANGVAVKK
                     PLAPVLPAELTTILAHPAALEESWPRVSRVRLRQK"
BASE COUNT          967 a         1383 c         1512 g          847 t
ORIGIN      
        1 aactcgactg gaatacgctg atcgccaaca aggaccgcga gattgcgcgg ctggacgcgg
       61 ttcatgacaa gagcgtccga gcggcagggg gcgagcctgt tcgttcgcat gccacgcttg
      121 tcgacaacca caccataagg ctggaagcgg agggccgtac cgtcacggcc gaccagatac
      181 tcatcgccac cggcagcagg ccggcggcgc atccagtaca tcctggccag gagcacgcca
      241 tcacgccaaa cgaggcgttc gatctgacgg aacttcccaa ggcgatcgtg atcgaaggcg
      301 acgactatat cgccgttgag tttgccaata ttttccatgg ccttggcgtc gagacaacgc
      361 ttgtctgccc aagagcggaa attctcagtg gtttcgatat gggcctgcgg caggaactgc
      421 atgaggcgat ggaaagaaaa ggcataaggg ttctctgtca ggccacctcc aagaaggtcg
      481 aaaggcagcc ggacggacgg cttgccatcg tggtcaacga cgacaaagcc ctggtggcgg
      541 accaattgat ccttgctgcc ggccgccgtg cgaacaccga aatgctcgga ctggatgcgg
      601 caggcgtcaa gacggggaaa accggcgaga ttatcgtcga cgattattcc cggaccaatg
      661 tggagaatat ctgggcgatc ggcgatgtca ccaaccgcgt gcaactgacg ccggtggcga
      721 tccatgaggc gatgtgtttc gtggagacgg ccttcaaggg caatccgacc gcacctgatc
      781 atggcgctat tgccaccgca gtgttttcgc agccggaaat cggcaccgtg ggcttgtcgg
      841 agggtgatgc cggcaagcaa tttgcggacc tggaaatcta ccgcgccctg ttccggccca
      901 tgcggcacac gctttcaggc aaccaggaaa aaatgctgat aaaactggtg gtggacggcg
      961 cgagccggaa agtgctcggc gcgcatatcc tgggacccga tgcggccgag atggcgcaag
     1021 ttctggccat tccggtcaag gctgggctga ccaagggcga tttcgaccgc acgatggcgg
     1081 ctcatcctac cgccgcggaa gaactcgtga ccatgtataa gccgagctac cgggtgaaaa
     1141 acggcgaacg ggcgggctga cgcccatccg ccggaacgtt tccctaattt agtcggccgg
     1201 cggcagcgcg cggccggctg cgccgaaaaa ccgcgtgcga tcctgttctt cggccctcgt
     1261 tttcatacga acgcgcatca ggtctttcca gccgacatag ccgacaaggc ggttgtcggc
     1321 gcgcgcaacc accggcaggt gcgagacgtt tgccgtctgc aacttatcgg caaggccttc
     1381 gagatattcg tcgggatagg ccagcgtaat cttggttccg ccaagcagtt cggcaagcgt
     1441 cgtcttgcga tgccggccgg cgcgtcgcca gcgcaggatg gccggcgggt cgacgatgcc
     1501 gagcacgcgg ccgtccggat cgaccaccgg aaagcttgga tggtgtgttt cgggcgaggt
     1561 caggtaggcg gccgcgccat gcagtgtcat cgacgaagaa accgtctgga cgtttttggt
     1621 catgacctcg ctgacgcggg tgagggcgaa cggatcgacg cggtattcgc gcaccaggtg
     1681 caggccgcgc ctggcgatct tttcggtcag gatcgagcgc ttcatcaaca gcaccgtcac
     1741 cgcatgggca gccgcgcagg ctgtgatgag cggaaccagg acatgggtgt tgccggtcag
     1801 ttcgaccgca aagaaggtgg cggtcagcgg cgcgcgcatc gtgccgccca tcgtcgccgc
     1861 catcgccagc agcgcccaga agccgggatc ggcaaccggc aggaagccgc caaggaccac
     1921 gcccatggcg ccgccgatga tcagcagcgg cgccagcacg ccgcccgacg ttcccgaccc
     1981 aagggcgacc gaccagatga tcgtcttgac taccagcaac aggatggctg ccttcgccag
     2041 ggtctcgccg ttcaacatcc gggcgatgtt gtcataaccg acgctgagcg cgttcggttc
     2101 gataagccca ccaagaccga cgatgaggcc gccgatcatc ggccaccaca tccaatggat
     2161 gggcagcttc aggaaggcat cttcgcaggc gtagacggct tgggtgagga ggccggaaag
     2221 cagcccgctg gcgatgccga tcgcgaccca gccgccaaga gcggcaaagg aagtttccat
     2281 gccgccattg aatgcgaata gcggcgaggg cagatggagc agcgtacgct cgacttcggc
     2341 gatgatcgcc gccacggcaa ccggaatgaa gctgcgcggc gtccattcga acagcaggag
     2401 ttcaacggcc agcatgatgg ccgcgatagg cgtgccgaaa acagtcgtca tgccggcggc
     2461 ggcgcccgcg accaacagcg tcttgcgctc attgtcggtg acaggcagcg cctgtgcgat
     2521 cagcgagccg atggcgccgc ccgtcatgat gatcgggcct tcggcgccaa aggggccgcc
     2581 ggtgccgatg gagatcgcag atgaaagcgg cttcaggatc gcgaccttgg cgtcgagccg
     2641 cgagcggcca agcaaaatgg cctccagcgc ctctggaatg ccgtggccac ggattttctc
     2701 gctgccgtaa cgcgccatca ggccgatgac caacgcgccg accaccggaa caagcaccgc
     2761 ccacaggccg agcggcgtgt cggcaagctt gaggtcttgt agggaaaatt ggccgaaata
     2821 ggccagattg gtgacaagct tgatgagctt tatcagaagg atgccggcgc caagcgccgc
     2881 cgttccaacg attacggcga ttgccgcgat gagcaaaacc cgtgcatctg tggtgaaatc
     2941 gccgagatgg cgttggtcgg cagggtccga tttcattcgc gaagttcttt cttggatcgg
     3001 ctatgttcaa tatggttttc gagcaggcgg ggggcttgtc gaagtctaag gccgccctct
     3061 atcgtaatac gatataaaaa cacaagcgat tatggcttgg gggcatgaat gagcgtagaa
     3121 aaatcggcag gaaagcaacg gtctcaaatc acgcaggccg attacgaggc gctttcggat
     3181 tttcgctatc ttctgcgccg cttcctggaa ttcagcgaaa aagccgcccg tgacgccggc
     3241 ttgcagccgc gccagcatca ggcgctgctc gcgatcaaag gcatgggaga gcgaggaccg
     3301 gtcggcgtcg gggatttggc ggaacggctg tgcatccgcc atcacagcgc cgtcgaactg
     3361 gtcaaccgcc tgggcgaagc cggtctggtt tcgcgcgagc atgacaaggt ggatcagcgg
     3421 cgggtgatcc tccatctgac ggagcgggcg gaggcgtatc tggcggggct gtccgccgcg
     3481 catctcgatg aattgtcacg catcgaaccc atgctgacgc gcattctttc ccacagcaaa
     3541 ggctagccgg taggacaagg ccggggcggc ggcattcggc gcggacgtcg cagccgatac
     3601 tatttctgcc gcagcctcac gcgggatacg cgcggccagc tttcttcaag cgccgctgga
     3661 tgggcaagga tggtcgtaag ctcggccggc agcacaggcg cgagtggctt cttgaccgca
     3721 acgccgttgg caatcatcag cacgctgttg tagaaatcga caccgtccgc cgattttggc
     3781 gcgacggctt catgcatgat gctgatgacg gtgacgttcg ggcaattgtc cttgatggcc
     3841 tggtggaagg cgattttgcc ttccgggtcg agcgccccgg tcgcttcgtc gaggaacagc
     3901 agtccgggcc gctggacgag gatgcgcgcc accaccagct tctgcttctg gcccccggaa
     3961 agaagctggt cccagctttt gccttttcgg ctttcctggt ccagatgctc gatgaaatct
     4021 cccaatccgg ccttgtgcag tgcggacgcc acctgtggat cggtatggca tgcgggcgtg
     4081 tccggcaggc agacaagttc cttcagcgac aaaggtggca acttcacttc ctgcgccgca
     4141 tagaaggatt tcaccccctc ggggaaaacg atcgtgcctt gcccataaga ccatagaccg
     4201 ttgatggcct tgaccagcga ggtcttgccg caaccggatt cgcccttgat gaaggtccat
     4261 tcgccgcgtt tgaagcgcag ccgttcggcg ctcaggaagg gaaccgtgtc atggccctgc
     4321 tggcgcagtt caaggttttg aacgctaagg ccgaataccg ggttctggcg aacataggtg
     4381 aaatcggaac tgccggtctg cttgtagaac tcgctcgggt gctggacgtt ctcgatcgcc
     4441 ttagcaagat cggtaacgcg cgtgctgttg gcccggagcg cggcgattgc cggcatcaca
     4501 tggatgaacc acgagcactg gctgatgagc gaattcacca gttcggcgcc ggtaatatag
     4561 cccttgagat cgacgcggct ggaaatgaac ggcagcagcc ccggcccata ggcaacgatg
     4621 cgcgcgccga cgaaattgta gatgcgctcg aacgagtcgt agccggcatt gacgacattg
     4681 atgcgcgccc atgtctggtc gatatcgac
//
LOCUS       DASYCS010000138         1827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4669894,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000138 DASYCS010000000
VERSION     DASYCS010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4669894"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1393
                     /locus_tag="VGM46_04650"
     CDS             <1..1393
                     /locus_tag="VGM46_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEY4191913.1"
                     /translation="VVGEIRETTAKGYRTLVTVLTKRMAEDLTEYLHEQGVRVRYMHS
                     DIDTLERIEILRDLRLGAFDVLVGINLLREGLDIPECGFVAILDADKEGFLRSETSLI
                     QTIGRAARNVDGKVILYADQITGSMERAMAETSRRREKQMEWNTANGITPESVKSRIS
                     DILDSVYEKDHVRADISQFTDAAGDMMGNNLKSHLDALEKQMRDAATNLDFEKAARIR
                     DEIKRLREMELSISDDPLAKYADMESPVSGREKGKHNKGVAKHRTVEEQERFRKLDAA
                     RADEAAAKAARPNLFAKPSLDEMGRSGDHSKPAGAGFKKPSLDDMGPGTDMPTPAGAV
                     SRSLFKKQSASEAHGSDFGIPGDAGRSPSGRRAAAPSRSEPQSDDSASESKPSLFRKN
                     SLDEMTVRRTEKPVEGAKPARPEAVADRSAPRDSDSVKPIVRQRVGVGSYEDPAEASR
                     QKRRPGKTGRPGK"
     gene            1493..>1827
                     /locus_tag="VGM46_04655"
     CDS             1493..>1827
                     /locus_tag="VGM46_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018428968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY4191914.1"
                     /translation="MPGDDQQPPFPPMPKALGGLTPYLGIEGATRAAEFYKKAFGAEQ
                     AFAVPPDDQGRTMHIHLHINGSSLMLGDFYPEHGHPAKAVQACTMQLHLTEQTIDDWW
                     RRAVDAGCT"
BASE COUNT          393 a          586 c          573 g          275 t
ORIGIN      
        1 tgtcgtcggc gaaatccgcg agacgaccgc caagggctac cgcacgctgg tgacggtgct
       61 gaccaagcgc atggccgagg atttgaccga atatctgcat gagcagggcg tgcgcgtgcg
      121 ctacatgcac tccgacatcg atacgctgga acgcatcgaa attctgcgcg atctgcgtct
      181 cggcgcgttc gacgtgctgg tgggcatcaa cctgttgcgc gaaggcctcg acattcccga
      241 atgcggcttc gtcgccattc tcgacgccga caaggaaggc ttcctgcgtt cggaaacctc
      301 gctgatccag acgatcgggc gcgcggcgcg taacgtcgac ggcaaggtca ttctctatgc
      361 cgaccagatc accggctcaa tggagcgggc gatggcggaa accagccgcc gccgtgaaaa
      421 gcagatggaa tggaacaccg ccaacggcat caccccggaa tcggtcaagt cccgcatctc
      481 cgacatactg gattcggtct acgaaaaaga ccatgtccgc gccgacatct cacaattcac
      541 cgatgccgcc ggcgacatga tgggcaacaa cctgaaatcc catctggacg cgctcgaaaa
      601 gcagatgcgc gatgccgcca ccaatctcga tttcgagaag gccgcgcgca tccgcgacga
      661 gatcaagcgg ctgcgtgaaa tggaactgtc catttccgac gacccgctgg cgaaatatgc
      721 ggatatggaa agccccgttt ccggccgcga aaaaggcaag cacaacaagg gcgtggcgaa
      781 acatcggacg gttgaggagc aggaacggtt ccgcaagctc gatgcagcgc gcgccgatga
      841 ggcggcggcg aaggccgcgc ggccgaattt gttcgccaag ccctcgctcg atgaaatggg
      901 gcgctccggc gatcattcga agcccgccgg cgccggcttc aagaagcctt ctctggacga
      961 catgggaccg ggcaccgaca tgccgacgcc ggccggcgcg gtttcacgtt cgctgttcaa
     1021 gaagcagtcg gccagcgaag cgcatggctc ggatttcggc attcccggcg atgccggacg
     1081 aagcccgagc ggccggcggg ctgccgcgcc gtcgcgaagc gagccacaga gcgacgacag
     1141 cgcgagcgaa agcaagccca gcctgtttcg caagaactcg ctggacgaga tgacggtgcg
     1201 gcgcaccgaa aagccggtcg aaggcgccaa gccggcgagg ccggaagcgg tcgccgaccg
     1261 ttctgcgccg cgagacagcg acagcgtcaa gccaatcgtt cgccagcgcg tcggcgtcgg
     1321 ctcctacgaa gaccctgccg aggcgtcgag gcaaaagcgc cgccccggca agaccgggcg
     1381 gccgggaaag tagatttttc gggatagccc gaaaaatctt ctccttcttg tcggatcggg
     1441 cgttcaccac gcgtcctgtg gaggtggaaa ccgccaaaga caggagaaca gcatgcccgg
     1501 tgacgaccag cagccaccct ttccgccgat gccgaaagcg ctcggcggcc tgacgcccta
     1561 tctgggcatc gaaggcgcga ccagggcggc ggaattctac aagaaggctt tcggcgccga
     1621 acaggcgttt gccgtgccgc ccgacgacca gggccgcacc atgcatatcc acctccatat
     1681 caatggctcc agcctgatgc tcggcgactt ctatcccgaa cacggccacc cggcgaaggc
     1741 cgtgcaggcc tgcaccatgc agcttcattt gaccgaacag accatcgacg attggtggcg
     1801 gcgcgcggtc gatgccggct gcaccat
//
LOCUS       DASYCS010000139         2169 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3798746,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000139 DASYCS010000000
VERSION     DASYCS010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3798746"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VGM46_04660"
     CDS             complement(<1..333)
                     /locus_tag="VGM46_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191915.1"
                     /translation="MSRRLFPAPGHTIIQQDNGHVSYWQKEYHQSAADGRGVSTADYI
                     HAMYFFLMQAGSKDVLMIGCGGGTLATMLRASNVRVTVVDLHQMSFDIARDYFHLPAD
                     VPCHVADGV"
     gene            complement(330..1508)
                     /locus_tag="VGM46_04665"
     CDS             complement(330..1508)
                     /locus_tag="VGM46_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191916.1"
                     /translation="MSKVFSILLSVSALFALPLAAHAAETPTNSIEALQAKLDAGTVK
                     LTFANDGHGWLQSVLAALGVAQDSQLLTFTRSSLQFDKISPKTPRAIYFGDDVAVAAV
                     VEGHVLEILANDAKGDPAFYTLDTAKTDKPKFEREGPRCLSCHGMVNTYAPGWMVANI
                     TAIADGTPQFPDVARPFYFTEQSTPFEERWGGWYVTGTHGAMHHRGNVTAKDLSHPYE
                     LPMIEGQNVTDLSGRFDTHQTLQPGSDIVALMTLEHQNGFANRVAMLNARAAGSQAEL
                     DKAIDEIVANMTFADEVPLPSPVKGTSSFTDTFPKRGPRDAQGRSLRDFDLKTRLFRY
                     PLSYMVYSASFDSLNPAVKTKLWRRLYDRLRATPDGAAAIAILAATKPDVPDYWKKGA
                     "
     gene            complement(1593..>2169)
                     /locus_tag="VGM46_04670"
     CDS             complement(1593..>2169)
                     /locus_tag="VGM46_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEY4191917.1"
                     /translation="AMVFSHGAINTVAASLFKIANDIRLLGSGPRSGLGELSLPENEP
                     GSSIMPGKVNPTQCEAMTQVCVQIFGNNAALTFADSQGHFELNVYNPVMAYNFLQSVR
                     LMADAAVSFTDNCVVGIAPRLDNIKAGLERSLMLVTALAPKIGYDNAAKIAKTAHKNG
                     TTLKEEAVGGGYVTAAEFDAVVKPEDMIAPK"
BASE COUNT          368 a          667 c          704 g          430 t
ORIGIN      
        1 gacaccatcg gcgacatggc agggcacatc ggcgggcaaa tggaaataat cccgcgcgat
       61 gtcgaagctc atctggtgca gatccacaac cgtcacgcgc acgtttgagg cgcgcagcat
      121 ggtggccaag gtgccgccgc cgcagccgat catcagcacg tctttcgagc cggcctgcat
      181 caggaagaaa tacatcgcat ggatgtaatc ggcggtgctg acgccgcgcc cgtcggcggc
      241 gctctggtga tattccttct gccaatagga aacgtggccg ttgtcctgct ggatgatggt
      301 gtggccgggc gcggggaaaa ggcggcggct catgcgccct tcttccaata gtccggcacg
      361 tccggcttgg tcgcggccag gatggcgatg gccgctgcgc catcgggcgt ggcgcgcagc
      421 ctgtcgtaca gccgccgcca caatttcgtt tttacggcag ggttcaggct gtcgaacgag
      481 gcactgtaga ccatgtagct gaggggatag cggaacagcc gcgtcttcaa gtcgaaatcg
      541 cgcagcgaac ggccctgggc gtcacgcgga ccgcgtttcg ggaatgtatc cgtgaagctg
      601 gaagtgcctt tgacgggcga tggcagcggt acttcgtcgg caaaggtcat gttggcaact
      661 atctcgtcga tcgccttgtc cagttcggcc tgcgatccgg cggcgcgcgc attcagcatg
      721 gcgacgcggt tggcaaagcc gttctgatgt tccagcgtca tcagggcaac gatgtcgctg
      781 ccgggttgca aggtctggtg cgtatcgaag cggccggaca ggtcggtgac gttctggcct
      841 tcgatcatcg gcaattcgta aggatggctg agatccttcg ccgtcacatt gccgcgatga
      901 tgcatggcgc cgtgcgtgcc ggtgacgtac cagccgcccc agcgttcctc gaacggcgtg
      961 ctctgttcgg tgaaatagaa gggccgtgcc acgtcgggaa attgcggcgt gccgtcggcg
     1021 atggcggtga tgttggccac catccagccg ggcgcatagg tgttgaccat gccatggcac
     1081 gacagacagc gcgggccttc gcgctcgaat ttcggcttgt cggtctttgc cgtgtccagt
     1141 gtgtagaagg ccgggtcgcc cttggcgtca ttggccaata tctccagcac atgaccttcc
     1201 accacggcgg cgaccgccac atcgtcgccg aaataaatgg cgcgcggcgt cttgggactg
     1261 attttgtcga attgcagact gctgcgggta aaggtcagaa gctggctgtc ctgcgccacg
     1321 cccagcgccg ccagcacgct ttgcagccag ccatggccgt cattggcgaa tgtcagcttg
     1381 accgtgccgg cgtcgagctt tgcctgcagc gcctcgatgc tgttggtcgg cgtttcggcg
     1441 gcgtgtgccg ccagcggcag ggcaaacagc gccgatactg acaacagaat ggaaaaaacc
     1501 ttactcactg ccgcacagcc gctcgcccaa gctcgcggcg ttcgtcgctt tcgcaggtcc
     1561 gccggacctg ctcacgcctg cggcgccgct cctcacttcg gcgcgatcat gtcttcgggc
     1621 ttcaccacgg cgtcgaattc cgccgccgtg acatagccgc cgccgacggc ttcttccttc
     1681 agcgtggtgc cgttcttgtg cgccgtcttg gcgatcttgg cggcgttgtc atagccgatc
     1741 ttgggcgcca gcgccgtcac cagcatcagc gagcgttcca gcccggcctt gatgttgtcc
     1801 aggcgcggcg cgatgcccac cacgcaattg tcggtgaacg acaccgccgc atcggccatc
     1861 agccgcacgc tttgcaggaa gttgtaggcc atcaccggat tgtagacgtt caattcgaaa
     1921 tggccctggc tgtcggcaaa ggtcagcgcg gcattgttgc cgaagatctg cacgcagacc
     1981 tgggtcatcg cctcgcactg ggtcgggttg accttgcccg gcatgatgga ggagcccggc
     2041 tcgttttccg gcagcgacag ttcgcccagg ccggagcgcg ggcccgaacc cagcaggcgg
     2101 atgtcgttgg cgatcttgaa cagcgacgcg gccaccgtgt tgatggcgcc gtggctgaac
     2161 accatggcg
//
LOCUS       DASYCS010000140         7176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1914462,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000140 DASYCS010000000
VERSION     DASYCS010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 7176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7176
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1914462"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2540
                     /locus_tag="VGM46_04675"
     CDS             <1..2540
                     /locus_tag="VGM46_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018428358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY4191918.1"
                     /translation="CSVLYHLAKAGWTDIMLIERSELTSGSSWHAAGGFHTLNGDPNV
                     AKLQAYTVQLYKEIEEISGQSCSLHLTGGVMMADTPERMDFLRLAHAKGRYLGMDTEL
                     ITPSEAKAMFPLMDETNFVGAMWDPVEGHLDPSGTTIAYSKAAKKLGAEIVLRNRVVD
                     LTQQPDGTWNVVTEQGTVHAEHVVNCGGLWAREIGRMVGVELPVLAMEHMYLLTEPMP
                     EVEAFNKETGREMVGVLDFKGEIYTRQERNGILLGTYEKACKPWSPVNTPWDFGHELL
                     APDLDRIAPSLELGFKHFPGIANAGIKQVINGPFTFAPDGNPLVGPVQGLTNFWCACG
                     VMAGFSQGGGVGLALSNWMVNGDPGFDVWGMDVARWGEWASLRYTNAKVRENYSRRFS
                     IRFPNEELPAARPAQTTPLYDTMLANNAVMGDSWGLETPLWFAPKGTKPKDVVSFRRS
                     NDFGPIGEEVRAVRERVGVTEIANFAKYEVSGTGSQDFLNRLMTNRMPKKGRLVLTPM
                     LNEFGKLIGDFTIANMGAKGVGDDRFMIWGSSAAAKYHMRWFEKHLPKDGSVRIHRFD
                     QTLVGLSIAGPKSQELLQKLVDVDVSSKAFRFMDFREMAVAGAPCMVNRITYTGDLGY
                     EIWMAPAYERLVYKAIKDAGEEFGLVDFGMRALLSMRLEKNFPTWFRELRPIYGPFEG
                     AMDRFVKMEKNDFIGRDAAAREQEAGPKLRRVSFVVDATDADVMGDEPIWAKVSKDYG
                     TVGKTHDFGAPRFDAKGKEVRGSKAAEGAAAVRGIVDGDWRVVGWVTSGGYAHHVRKS
                     MAQGYVPAALAENESADLFEIEILGQRCAARINVEPPFDPSGEKMRG"
     gene            complement(2669..3451)
                     /locus_tag="VGM46_04680"
     CDS             complement(2669..3451)
                     /locus_tag="VGM46_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006335506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HEY4191919.1"
                     /translation="MSEFIASLPMYDWPELRAETDAYWARLRDVLRSRGIDAPDALVR
                     RNGDLPPVPTGIRDARGEVVAPDPATLAPDEFDLHALWLHPALLVAQTCWGPMGQGLA
                     AHVQVVGQPDYSAFEGGEGELYSSAIVMRGRNSVAASGKPDIPLGLLRGKHFIYNSPD
                     SMSGILALAQDLEAVGETLDVFSERGLSGGHRASIIAVADGRADIAAIDCRSWAMAQF
                     FEPKAKELAAVGWTARRKGLPFITARATPVDVVAALSDVLRS"
     gene            complement(3528..4535)
                     /locus_tag="VGM46_04685"
     CDS             complement(3528..4535)
                     /locus_tag="VGM46_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEY4191920.1"
                     /translation="MKKTSRAKSAKAIEWPTVILTVSCYGVWLVVGMLVWPSWPVLAL
                     AILTITVALQSSIVHEVLHGHPTRNALVNEAFVFLPIGLIWPFRRFRTLHLRHHCDER
                     LTDPLDDPESYYQALWRHDELPAAMKMLLKINNTMAGRFVLGPWLAVIGFVIDDIKQM
                     LAGDKTIQKAWLLHGTGVAMVVPVITYGFGMPLWLYILVPTWLGQSLIAIRTFAEHQW
                     SERPEGRTIIVERSPLALLFLNNNLHFVHHKAPTTPWYGLPKLFQDRREDWLKANNGY
                     VYPNYFALVKAYAFRAKEPVAHPALRRAPEPGRAFRPRVHASNRSELGSSPVPAKPSK
                     E"
     gene            4762..5610
                     /locus_tag="VGM46_04690"
     CDS             4762..5610
                     /locus_tag="VGM46_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY4191921.1"
                     /translation="MDKRDLSTLFRERLKMLLIRSDLNQSTFAAAVGIDRSALSQLLS
                     GASTRLPRAETLLNIAAEFKVSLDWLLGLSQDEGLTGEIRESVEIEEGSGSFDRTLLA
                     KWHAEAAGTKIRYVPTGLPDLLRTGALVEYESGIANRSHEAQIEETQYRIDYNRRPET
                     DMEVCMPRHTLEIFARGLGVWSGFPEADRREQLRHMAALLDDLYPTFRLFLYDGRLRY
                     SIPYTIFGPYRAAIYVGGMYLVLNATTPIQTLTRHFDNLIRAAEINPHEAASFAHTLA
                     DARFDD"
     gene            5687..6706
                     /gene="bmt"
                     /locus_tag="VGM46_04695"
     CDS             5687..6706
                     /gene="bmt"
                     /locus_tag="VGM46_04695"
                     /EC_number="2.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine--homocysteine S-methyltransferase"
                     /protein_id="HEY4191922.1"
                     /translation="MTTNPIDELLAEKGVLLADGATGTNLFAMGLQAGEAPELLNETA
                     PETIASLHRNFIEAGADIILTNSFGGTRQRLKLHNAQDRVHALNKRAAEIARAVADKA
                     GRKVIVAGSVGPTGELLVPLGAMTYDEAVESFAEQIAGLKEGGAEVAWIETMSAPDEI
                     RAAAEAAIRVGLPYTYTGSFDTAGRTMMGLLPKDIHGVADGLAQAPVGVGANCGVGAS
                     DILASLLDMTEARPEATVIVKGNCGIPEFRGSEIFYSGTPELMSDYVRLAVDSGAKII
                     GGCCGTSFEHLAAMRKALDSHNRQGRPSVETIIERIGPLRNKAAQENIAETAQGRRER
                     RRTRV"
BASE COUNT         1440 a         2289 c         2149 g         1298 t
ORIGIN      
        1 gctgctcggt gctctatcac ctagccaagg ccggctggac cgacatcatg ctgatcgagc
       61 gctcggagtt gacctccggc tcgtcctggc acgcggcggg cggcttccac acgctgaatg
      121 gtgaccccaa cgtcgccaag ctgcaggcct acaccgtgca actttacaag gagatcgagg
      181 aaatctccgg ccagtcctgc tcgctgcatt tgaccggcgg cgtgatgatg gccgatacgc
      241 cggagcgcat ggacttcctg cggctggccc acgccaaggg ccgctatctc ggcatggaca
      301 ccgagctgat cacgccttcg gaggccaagg cgatgttccc gctgatggac gagaccaatt
      361 tcgtcggcgc catgtgggat ccggtcgaag gccatctcga tccctcgggc acgaccatcg
      421 cctattccaa ggcggccaag aaactcggcg ccgagatcgt gctgcgcaac cgcgtcgtcg
      481 acctgacgca gcagcccgac ggcacctgga acgtcgtcac cgaacaaggc acggtgcatg
      541 ccgagcacgt cgtcaattgc ggcggcctgt gggcgcgcga gatcggccgc atggtcggcg
      601 tcgaactgcc ggtgctggcg atggagcata tgtacctgct gaccgagccg atgccggagg
      661 tggaagcctt caacaaggaa accggccgcg aaatggtcgg cgtgctcgat ttcaagggcg
      721 agatttacac ccgccaggag cgtaacggca tcctgctcgg cacctatgaa aaggcctgca
      781 agccgtggtc gccggtcaac acgccatggg acttcggcca tgaactgctg gcccccgacc
      841 tcgaccgtat cgcgccctcg ctggaactcg gcttcaagca ttttccgggc atcgccaatg
      901 ccggcatcaa gcaggtcatc aatggcccct tcactttcgc gcccgacggc aacccgttag
      961 tcggcccggt gcagggcctg accaatttct ggtgtgcctg cggcgtcatg gccggttttt
     1021 cgcaaggcgg cggcgtcggt ctggcgctgt ccaattggat ggtcaacggc gatcccggct
     1081 tcgacgtatg gggcatggat gtcgctcgct ggggcgaatg ggcgagcctg cgctacacca
     1141 acgccaaggt gcgcgaaaac tattcgcgcc gcttctccat ccgctttccc aatgaggaat
     1201 tgccggcggc gcgcccggcg cagaccacgc cgctctacga caccatgctc gccaacaacg
     1261 ccgtgatggg cgatagctgg gggctggaaa cgccgctctg gttcgcgccg aagggcacca
     1321 agccgaagga tgtcgtctcg tttcgccgct ccaacgactt cggcccgatc ggcgaggaag
     1381 tgcgcgcggt gcgcgagcgc gtcggcgtca ccgaaatcgc caatttcgca aaatatgaag
     1441 tcagcggaac gggttcgcag gatttcctga atcggttgat gaccaaccgc atgcccaaga
     1501 agggtcgctt ggtgctgacg ccgatgctca atgagttcgg caagctgatc ggcgacttca
     1561 ccatcgccaa tatgggcgca aaaggcgtgg gcgacgaccg cttcatgatc tggggttcgt
     1621 cggctgccgc caaataccac atgcgctggt tcgaaaaaca cctgccgaag gacggttcgg
     1681 tgcgcatcca ccgcttcgac cagacattgg tcggtctttc catcgccggc ccgaaaagcc
     1741 aggagctgct gcaaaaactg gtcgatgtgg acgtttccag caaggctttc cgcttcatgg
     1801 atttccgcga gatggcggtc gccggcgctc cctgtatggt caaccgcatc acctataccg
     1861 gcgatctcgg ctacgagatc tggatggccc cagcctatga gcggctcgtc tacaaggcaa
     1921 tcaaggacgc cggcgaagaa ttcggcctcg tcgatttcgg catgcgcgcg ctgctttcca
     1981 tgcgtctgga aaagaacttt ccgacctggt tccgcgagct gcgccccatc tatggcccgt
     2041 tcgaaggcgc gatggaccgc ttcgtcaaga tggaaaagaa cgacttcatc ggccgtgacg
     2101 cggccgccag ggaacaggaa gctggcccga agctgcgccg cgtatccttc gtggtcgacg
     2161 ccaccgatgc cgatgtaatg ggcgacgagc cgatctgggc caaagtttcc aaggactacg
     2221 gcacggtcgg aaaaacccat gatttcggcg cgccgcgctt cgacgccaag ggcaaggaag
     2281 tgcgcggctc aaaggctgcc gaaggcgcgg ccgccgtgcg cggcatcgtc gatggcgact
     2341 ggcgcgtggt cggttgggtg acttccggcg gctatgcgca ccacgttcga aaatcgatgg
     2401 cgcagggcta tgtgcccgca gccttggcgg agaacgagag cgccgacctg ttcgagatcg
     2461 agattctcgg gcagcgctgc gccgcccgca tcaatgtcga gccgccattc gacccatcag
     2521 gcgaaaagat gcggggctaa ccccagcccg cctaccactt cgatatcggc ccgctacgat
     2581 atcggcgatg ccggtcgttt tcatgcctga agcggccgaa agcaaaaaca gagttgcgcg
     2641 gcgccacgaa aaaagggctg ggccaagcct acgaacgaag cacgtccgac agcgccgcca
     2701 cgacatcgac cggcgtggcg cgcgccgtga tgaacggcag gccttttcgg cgcgccgtcc
     2761 agcccaccgc ggcaagctcc tttgccttcg gctcgaaaaa ttgcgccatc gcccagcttc
     2821 ggcaatcgat cgcggcaatg tcagccctgc cgtcggcgac cgcgatgatg gaagcgcgat
     2881 gcccaccgct caagccccgc tcgctgaaaa cgtcgagcgt ctcgcccacc gcctccaaat
     2941 cctgcgccag tgcaaggatg cccgacatgg aatcggggct gttgtagatg aagtgcttac
     3001 ccctcaacag acccagcgga atgtctggtt tgccgctggc cgccactgag ttgcgcccac
     3061 gcatgacgat agcgctcgaa taaagctcgc cttctccgcc ttcgaaggct gaataatcgg
     3121 gctggccgac gacttgcaca tgggccgcca gcccctgccc catcggcccc cagcaggtct
     3181 gcgcgaccag caaggccgga tgcagccaca aggcatgaag gtcgaattca tcgggcgcaa
     3241 gtgtagccgg atcgggcgcg accacttcgc cgcgagcatc gcggatgccc gtcggcaccg
     3301 gcggcagatc accgtttcgc cggaccagcg cgtccggagc atcgatgccc cggcttcgca
     3361 aaacgtcgcg caaccgtgcc caatacgcgt cggtttccgc ccgcaattca ggccagtcgt
     3421 acatcggcag ggacgcaatg aattcgctca tgctgccatg tgaccgtttt ggctttcatc
     3481 tggcaaggcc acgaccggca tcgtcagccg cctgctcacg aaagaaatta ctctttcgac
     3541 ggctttgccg gaacaggcga ggagccaagc tcgctgcgat tgcttgcgtg cacgcgcggc
     3601 ctgaacgccc tgccaggttc cggcgcgcgg cgcagcgcgg gatgcgccac cggctccttg
     3661 gcccggaagg cataggcctt caccagcgcg aaataattcg gatagacata gccattattg
     3721 gctttcagcc aatcctcacg gcggtcctga aaaagcttcg gcagtccata ccacggcgtg
     3781 gtcggggcct tgtgatgcac gaaatgcaaa ttattgttga gaaacagcag cgccagcggc
     3841 gagcgctcga cgatgatcgt gcgcccctcc ggccgctccg accactggtg ttcggcgaaa
     3901 gtgcggatcg cgatcaacga ctggccaagc catgtcggca cgagaatata gagccatagc
     3961 ggcatgccga agccataggt gatgacagga acaaccatgg ccacgccagt tccatgcagc
     4021 agccatgcct tttggatagt tttgtcgccc gccagcatct gcttgatgtc gtcgatgacg
     4081 aagccgatca ccgccagcca gggtcccaaa acgaagcgtc cagccatggt gttgttgatt
     4141 ttcagcagca tcttcatcgc cgccggcagt tcatcgtgcc gccacaaggc ctgataatag
     4201 ctttccggat cgtccagagg atcggtcagt cgctcgtcgc aatgatggcg caggtgcagc
     4261 gtcctgaagc gtcggaacgg ccagatcagc ccgataggca gaaagacgaa cgcttcattg
     4321 accagtgcgt tgcgggtcgg atggccgtgc agcacttcgt ggacgatgga ggattgcagc
     4381 gcgaccgtga tcgtcaggat ggcgagcgcc agcaccggcc atgatggcca gaccagcatg
     4441 ccgacaacca gccagacgcc ataacaggaa acagtgagaa tgaccgttgg ccactctatc
     4501 gccttcgccg atttcgctct gctcgtcttt ttcatgccac cgatcactgc ccccagcacc
     4561 ggaatcgccc accgaattcc tgacagcatt gtgtcagcag cattttgcaa cctcaacgcg
     4621 attaagcgca caagatgttg cgattgcgca tcttaagccg cagatgttat gtattgtaaa
     4681 caaacatggg attcatttac gctcatggcc atgctttcaa gggccagctg aaaatctatc
     4741 ctctaaaata cgagaaccga tttggacaaa cgcgacctct ccactttgtt ccgcgagcgg
     4801 ctaaaaatgc tgctgatacg ctccgacctc aaccagtcca ccttcgcggc tgcggtcggc
     4861 atcgaccggt cggcgctgtc gcagcttctt tcaggcgcat ctactcgcct tccccgagca
     4921 gaaacgctgc tgaatatcgc agccgagttc aaagtgtctc tcgactggct gctgggcctt
     4981 agccaggacg aaggcctaac cggcgagatt cgcgagagcg tcgagatcga ggagggctcg
     5041 ggcagcttcg accgtacgct gctggccaaa tggcacgcgg aggccgccgg cacgaagatc
     5101 cgctatgttc ccaccggcct gcccgacctt ctgcgcacag gggcgcttgt cgaatatgaa
     5161 tccggcatcg ccaaccggag ccacgaggcg cagatcgagg aaacgcagta ccgtatcgac
     5221 tataatcggc ggccagaaac cgacatggaa gtgtgcatgc cgcggcacac gctggaaatc
     5281 tttgcgcgcg gcctgggtgt ctggagcggc tttcccgaag ccgacaggcg cgagcagctg
     5341 cgccatatgg cggccctgct cgacgatctc taccccactt tccgcctgtt cctttatgac
     5401 ggccgcctgc gctactcgat cccctatacg atcttcgggc cgtaccgcgc cgccatttat
     5461 gtcggcggca tgtatctcgt gctgaatgcc acgactccga tccagacgct tacacggcat
     5521 ttcgacaatc ttatccgcgc cgccgagata aacccccatg aggccgcctc cttcgcccat
     5581 acgctggccg acgcgcgttt cgacgattga tatgcccgcc agacattgac gacatataaa
     5641 gatttcctta tatcactagc gcgcaaaaat tccggaaggc acgcccatga cgaccaaccc
     5701 gatcgatgaa ctgctggctg aaaagggcgt gctgctggcg gatggcgcca ccggcaccaa
     5761 tcttttcgcc atgggattgc aggcgggcga agcaccggaa ctgcttaacg aaacagcccc
     5821 cgaaaccatc gccagcctgc accggaattt tatcgaagcc ggcgccgaca tcatcctcac
     5881 caattccttt ggcggcaccc gccagcgcct gaaattgcat aacgcgcagg accgcgttca
     5941 tgcgctcaac aagcgcgccg ccgagatcgc acgcgccgtt gccgacaagg cggggcgcaa
     6001 ggtcattgtc gccggctccg ttggcccgac gggcgaattg ctcgtaccgc ttggcgccat
     6061 gacctatgac gaggctgtgg aatccttcgc cgagcagatc gcgggcctga aggaaggcgg
     6121 cgcggaagtc gcctggatcg aaaccatgtc cgcgccggac gaaatccgcg cggcagccga
     6181 agccgccatc cgcgtcggcc tgccctacac ctataccggc tcgttcgaca ccgcaggccg
     6241 caccatgatg ggcctgctgc cgaaggatat tcacggcgtc gccgatggcc ttgcgcaagc
     6301 gccggtcggc gttggcgcaa attgcggcgt cggcgcttcg gatattctgg cctcgctgct
     6361 cgacatgacc gaagccaggc cggaagcgac ggtgatcgtg aaaggcaatt gcggcattcc
     6421 cgaattccgc ggctcggaaa tcttttattc gggcacgccc gaactgatgt ccgactatgt
     6481 gcgtctggca gtggatagcg gcgcgaaaat catcggcggc tgctgcggca cgtcgtttga
     6541 acatctcgcc gcgatgcgca aggcgctcga cagccataac cggcagggcc gcccaagcgt
     6601 cgagaccatc atcgagcgca tcgggccgct gcgcaacaag gcggcgcagg aaaatatcgc
     6661 cgaaacggcg caagggcgtc gcgaacgacg ccgtacccgc gtctagggcc gctttaccga
     6721 ttggatcgga gcattctgcc ggtaacggtc cgggacaaaa ccagggccat tgtcgaggat
     6781 cgcacctagt ggtgttgttg agatgaaggc caagggattc gacccggatc ggtcgggcca
     6841 ttgcggtact ctggccgagc ggccggatgg gacagggacg tgcgctttat tggctcgctc
     6901 gtccgctgaa ccactccgac ccgcgcaagc cttcgcgcga accgcctcac cgtccgccag
     6961 actgagtgac cctgtctacg gcgaaacggc ctacgccagt caatcgacct tattcgttct
     7021 cggcgtagct cgacagcacc gaattgtaat ccgcgccggg gccatcggcg tcgggtttca
     7081 tggtcatcag cgcgccggat gtgccggggt cggatagttg cttcagcatc gtcccgaagc
     7141 ggtcgttctt cagcgccgac agggcggtac tgcgcg
//
LOCUS       DASYCS010000141        10518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4670187,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000141 DASYCS010000000
VERSION     DASYCS010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 10518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10518
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4670187"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="VGM46_04700"
     CDS             <1..673
                     /locus_tag="VGM46_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY4191923.1"
                     /translation="GKQVASDVGGDFVAIDVGDAAGVDAAAQAIEARFGAVAGLVTSA
                     GVIQPPLPPEELDLGLWDRVVHIDQRGTYVTCLAFGRRMAARGTGGAIVNIASVAGSR
                     SMPLHSYAPAKAAVISMTACLASAWGRDGVRVNSVSPGYTATPGLQTEIDAGRRDVSR
                     LVGNAPLGRLVQPSEIGQAVAFLLSPAASAISGIDLPVDCGWLAGSTWATYGGLPSPQ
                     QGVAT"
     gene            670..1659
                     /locus_tag="VGM46_04705"
     CDS             670..1659
                     /locus_tag="VGM46_04705"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEY4191924.1"
                     /translation="MTRPAPAHGIAGNSRLLDLHRTMLLIRATETTLSKLFADSEIPG
                     FIHLSLGQEAVAAGVCCALGSADTLATTHRGHGHVIARGIDLDGFFKELMGREGGLCK
                     GRSGSMHVADMSLGILGANGIVGASVPLALGSALSHQTLKTGGIAVAFFGDGAMAEGA
                     LHEALNMAALWSLPMVFACENNGWSEFSPTSRQFKGKLSTLAGAFGIRHILVDGNDPE
                     AIASAALEAADHARTAGPAVLECMTTRFRGHYEGDPQKYRDAGEMSAMTEGDPILRGR
                     ARLAELGVEAAELDAMAAQAEALVAAAVEVARADPLPDFDATLADVYTPLEAR"
     gene            1661..2635
                     /locus_tag="VGM46_04710"
     CDS             1661..2635
                     /locus_tag="VGM46_04710"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019966160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEY4191925.1"
                     /translation="MADTRYVRAINKALSDAMAADPSVILLGEDIADAGGSFKATRGL
                     LDQFGPQRVRDTPISESTIVGAAVGAALSGLKPVVEIMFMDFVTLAMDALVNQAAKSR
                     FMFGGQSSVPMVVRLPHGGGVNAGPQHSQCLEAWFAHVPGLKVVCPATVEDAYSLLRA
                     AIEDPDPVIFVENKALYAMAGDLPDAPGKGVIGKARIARAGSDVTLVTYGATLFNCLK
                     AADRLAGRDIFAEVIDLRSLQPWDKETVLASVARTHRAVVAHEAVRSFGAGAEIAAII
                     GEEGFDDLDAPVGRVGAPFMPVPFARSLESRYIPDADAIEAAVKVCLA"
     gene            2645..3448
                     /locus_tag="VGM46_04715"
     CDS             2645..3448
                     /locus_tag="VGM46_04715"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY4191926.1"
                     /translation="MSDPAIMSDPTILRSIDGPVGTLTVNRPQALNALDVETLLAMER
                     ELTALEQDAAVRVIVFTGAGDKAFIAGGDIADLNSRQGLAHYLEFAEIVHRVFRRIEL
                     CDKPTICAINGWALGGGTELLLATDIRLAADHARLGLPEITLGLFPGAGGTQRLIRQV
                     SPCKAKELMFTGDRITATEALALGLVNRVVEKERLAEETTALAERIAQKSPLVLKLLK
                     RTLASGADMPLPSALAHEQAMIGLVLDTRDAHEGCAAFLEKRQPAFEGR"
     gene            3453..4682
                     /locus_tag="VGM46_04720"
     CDS             3453..4682
                     /locus_tag="VGM46_04720"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEY4191927.1"
                     /translation="MAIDLLMPKLGLTMTEGVLLEWKASPGDRVAKGQLLYVVETDKI
                     ATEVEAEAAGVLEAQMVGEGETAVVGAVLGRLAMEGEAAAPKQAAVAATAAPRAADAK
                     PAKLEALEALALARAPAGSAQRIVATPLARRIAQANAVDLHGVAGSGPRGRIKAMDVE
                     QAMSARPAQASGVPASGIPGGVTRSKPSATQAAMARRLAEVKHGVPHFYLSTEIEVSA
                     LLALRETLNADAQAPRLTLNHFVLAATGRALRDHPNINRVWADGELVAFADTDVSMAV
                     ETERGLFVPVVRNAGARPLDAVAADARALVARARGGGLAAADMAGGAIAVSNAGMHDV
                     TFVTSIINPGQSSILGIGSVRDLFRPDREGKPELRREMGVVFSLDHRVHTGVEGLAFL
                     SSLKAYLETPMRLLRQV"
     gene            4696..5868
                     /locus_tag="VGM46_04725"
     CDS             4696..5868
                     /locus_tag="VGM46_04725"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY4191928.1"
                     /translation="MTVELTEDQNMIVATARRVGADFGLDYWRDLDARKAYPTEAWKA
                     ICDAGLCGVALPEEYGGSGRGMFDMALIIEALAAGGAGSTIGQLFMCNPIFGGVAIAK
                     YGSDAMRSQLLPKLISGELQFCMALTEPDAGSNTLEMRAYAAPDGDGFRLNGQKIWIT
                     NVPESDKMLVVARTIKYGESERRTDGISIFLIDTDRAGISHAKIDKLGTNTLASSTVF
                     FEDVRIGRDEVIGTLHKGWPELLDILNAERIATTAGLVGTVDLAIGLATGYARERKLF
                     AGKPIAAYQGLQFPLAQAYAETECARLMNRQAATLFDEGKPFGVQSNIAKLIAAQACS
                     AATERAMQTMGGMGYAKEFHVERLWRDCRLFRFAPVSEEMILNYLAVHQLGMVRSY"
     gene            5901..7421
                     /locus_tag="VGM46_04730"
     CDS             5901..7421
                     /locus_tag="VGM46_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY4191929.1"
                     /translation="MVNLSSFIHFHALRQPDKLALVYGGERVSYAELDRRIGDTAAYL
                     AAQGIGPGDIVAVFMKNSAAFIEIALATSHLGAVFLPVNFRLSGDECAYIVAHAGAKL
                     LLADGEFAATVAGIANAVLVDEAAQACAGRLGAPGLPAPALHLRRPEDLFRLMYTSGT
                     TDRPKGVTHSYENFYWKCMDHVIALGMGEADRLLAVGPLYHVGAFDLPGIAILWVGGT
                     LVVQRQFDETQVLAIIEAERITCAWFAPMMVGRMLALAGTDAYDLSSFKWCIAGGEKT
                     PENRIREFTRLFSAARFIDGFGLTETCSGDTLMEAGKEIEKIGSTGRALAHVQITIRD
                     DGGQVLAPGVEGEICLRGPKVTKGYWRDAKKTEQSFYGDWFRSGDVGYLDEDDFLFIT
                     DRKKDMIISGGENIASSEIERVIYAMPEVAECAVIGMPDPKWGERPAAIIVVREGHGL
                     SGEEVQARCRAALASFKVPTRIFFRDALPRNPSGKVLKRVLRDETAAGDDKQEARP"
     gene            7418..8782
                     /locus_tag="VGM46_04735"
     CDS             7418..8782
                     /locus_tag="VGM46_04735"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY4191930.1"
                     /translation="MSADTQTLSQRIGAFVAGLSMETLPGGVVEKARICLFNGYGIGL
                     GCHSTPYADVARAAALGFDGERADGATLLGDGRKVAVNFAAIANSALFHGRAQEDTCG
                     AAHLGAIMIPLLTALLEARKLPVERLIPALVAGYETGGLLEKAYSPITTPRGLRSSPL
                     YGTLAAAAAAARLMGLDASRTAAAIDYAASFTGGILQSFSEGTDEWRYQVGVAGATGL
                     TAMLLAANGAVSATHSLEGRFGFARTFAGTDCDVDGLASRLGRDWAIERVTFKPYPVC
                     AFNQTPVIAALALKEKAAGRDFALIKVRMNPLETGYAGMDSTGPFHSISGTLMSIPFC
                     TALTLVRGEPTLKMMTTYDDGAVNALVSRVELVPDEGVSRLCCTIEAELAGGERIGIE
                     QVMTTENFNYDRATVSQLVRRIGAEQDVPARAYDLIEDFAFGLPDKGVGQVLSAFALC
                     PRPA"
     gene            8869..9516
                     /locus_tag="VGM46_04740"
     CDS             8869..9516
                     /locus_tag="VGM46_04740"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY4191931.1"
                     /translation="MSEKQQAIVVRRPRGQKPRREENAEAKFNALLHAACEIVGEYGY
                     VDASISRITERAGVAQGTFYSYFESRQDLFDQLLPKLGTEMLAFIKERTRGVVDASER
                     ERKGFMAFFDFIVQNPDFLRILNEAEQLAPKGHEAHFQLVVAGYLANLQRDWARGEYP
                     GYDERELEVIVYMLLSARSYLALRYAKDEDGTRPFPGWVTDTYMKFITNGLKAKP"
     gene            complement(9632..10264)
                     /locus_tag="VGM46_04745"
     CDS             complement(9632..10264)
                     /locus_tag="VGM46_04745"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008674 -
                     2-dehydro-3-deoxy-6-phosphogalactonate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0034194 - D-galactonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HEY4191932.1"
                     /translation="MSEAIAWPAFRRDLVAILRGVTPQEVEAIAGVLAQEKFEAIEVP
                     LNSPDALRSIEIVAKAFGKDVLVGAGTVLSPEDCQRVADAGGRLMVSPNVDPAVIARA
                     QACGMVSLPGVFTPTEALLALRCGASALKFFPAGALGASGISAIKAILPKDAVVGAVG
                     GVSELTFSDFARVGVRLFGLGTSLYRPGDIAEEVRNKAQLAVKAYDKVFR"
     gene            complement(10268..>10518)
                     /locus_tag="VGM46_04750"
     CDS             complement(10268..>10518)
                     /locus_tag="VGM46_04750"
                     /inference="COORDINATES: protein motif:HMM:NF016894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008671 - 2-dehydro-3-deoxygalactonokinase
                     activity [Evidence IEA];
                     GO_process: GO:0034194 - D-galactonate catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HEY4191933.1"
                     /translation="LGFEEKRQGAPRLSGLLIGSEIAAARSLYGAAGEIVLLASGALG
                     NLYQSALVEAGYNVVLANAEEAVRIGLAKAAARLWGKE"
BASE COUNT         1743 a         3581 c         3473 g         1721 t
ORIGIN      
        1 cggcaagcag gtggccagcg atgtcggcgg tgatttcgtc gccatcgacg tgggcgacgc
       61 ggccggcgtc gatgcggcgg cgcaggccat cgaggcgcgc tttggcgcgg tcgccgggct
      121 ggtcaccagc gccggcgtca tccagccgcc gctgccgccg gaagaactcg accttggcct
      181 gtgggaccgc gtcgtccata tcgaccagcg cggcacctat gttacgtgcc ttgcctttgg
      241 ccgccgcatg gcggcgcggg gcacgggcgg ggccatcgtc aacatcgcct ccgtcgccgg
      301 gtcgcgctcc atgccgctgc attcctatgc cccggccaag gcggcagtca tctccatgac
      361 cgcctgcctg gcttccgcct ggggccgcga cggcgtgcgg gtcaattcgg tttcgcccgg
      421 ctatactgcg acgcccggcc tgcaaacgga gatcgacgcc gggcggcgcg acgtttcgcg
      481 ccttgtcggc aacgccccgc tggggcggct ggtgcagcct tccgagatcg gacaggccgt
      541 cgccttcctg ctttcgcccg ccgcctccgc catttccggc atcgacctgc cggtcgattg
      601 cggctggctg gccggctcca cctgggcaac ctatggcggc ctgccctcgc cgcagcaggg
      661 cgtcgccaca tgacgcggcc ggccccggcg catggcatcg cgggaaattc gcggctgctc
      721 gaccttcatc gcaccatgct gctgatccgg gcgacggaga cgaccctgtc gaaactcttc
      781 gccgacagcg aaattcccgg cttcatccac ctgtcgctcg ggcaggaagc ggtggcggca
      841 ggcgtgtgtt gcgctcttgg ctcggccgat acgctggcca ccacccatcg cggccacggc
      901 catgtgattg cgcgcggcat cgaccttgac ggcttcttca aggagctgat ggggcgcgag
      961 ggcggcctgt gcaaggggcg ttccggctcc atgcatgtcg ccgatatgag ccttggcatc
     1021 ctgggcgcaa atggcattgt cggcgccagc gtgccgctgg cgctgggcag cgcgctttcg
     1081 caccagacgc tgaagaccgg cggcatcgcg gtcgccttct tcggcgacgg cgcgatggcc
     1141 gagggcgcgc tgcacgaggc gctgaacatg gcggcacttt ggtcgctgcc aatggtgttt
     1201 gcctgcgaaa acaatggctg gtcggaattt tctccgactt cgcgccagtt caagggcaag
     1261 ctttccaccc ttgccggggc tttcggcatc cgtcacattc tggtcgacgg caacgacccg
     1321 gaagctatcg ccagtgccgc gttggaggcc gccgaccatg cccgcacggc ggggccggcg
     1381 gtgctggagt gcatgaccac gcgctttcgc ggccattatg aaggcgatcc gcagaaatac
     1441 cgcgacgccg gcgaaatgtc ggccatgacc gagggcgacc ccatcctgcg cggccgcgcc
     1501 cggctggccg agcttggcgt cgaggcagcg gagctggacg caatggccgc gcaagccgaa
     1561 gcgctggtgg ccgctgccgt ggaagtcgcg cgcgccgacc cgctgcccga tttcgacgcc
     1621 acgctcgccg atgtctacac cccgctggag gcgcgctgac atggctgaca cacgctatgt
     1681 ccgcgccatc aacaaggcgc tttccgacgc gatggccgcc gacccctcgg tgatcctgct
     1741 gggcgaggac atcgccgatg ccggcggctc gttcaaggcg acgcgcggcc tgctcgacca
     1801 gttcggcccg cagcgcgtgc gcgacacgcc gatcagcgaa agcaccatcg tcggcgcggc
     1861 ggtgggggca gcgctcagcg ggctgaagcc ggtggtcgag atcatgttca tggatttcgt
     1921 cacgctcgcc atggatgcgc tggtcaacca ggcggccaag tcgcgcttca tgttcggcgg
     1981 gcaaagcagc gtgcccatgg tggtgcggct gccgcatggc ggcggcgtga acgccgggcc
     2041 gcagcattcg caatgcctgg aagcctggtt cgcccatgtg ccgggcctca aggtggtctg
     2101 cccggcgacg gtcgaggacg cctacagcct gctccgcgcc gcaatcgagg acccggaccc
     2161 ggtgatcttc gtggaaaaca aggcgctcta cgccatggcc ggcgacctgc cggatgcgcc
     2221 cggcaagggc gtcatcggca aggcccgcat cgcgcgcgcg ggaagcgacg tgacgctggt
     2281 gacctatggc gcgaccctgt tcaactgcct gaaagccgct gaccggctgg ccggccggga
     2341 catttttgcc gaagtgatcg acctgcgctc gttgcagccc tgggacaagg aaacggtgct
     2401 cgcttccgtc gcccgcaccc accgcgcggt ggtggcgcat gaggcggtgc gcagtttcgg
     2461 cgccggcgcc gaaatcgccg ccatcatcgg cgaggaaggg ttcgacgacc tcgacgcgcc
     2521 ggtcgggcgc gtcggcgcgc ccttcatgcc ggtgcccttc gcccgctcgc tggagagccg
     2581 ctacatcccc gacgccgacg ccatagaggc ggccgtcaag gtctgcctcg cctaggagcc
     2641 gcccatgtct gatcccgcta tcatgtccga tccaaccata ttgcgcagca tcgacgggcc
     2701 ggtcggcacg ctgaccgtca accgcccgca agccctcaac gcgctcgatg tcgagacgct
     2761 gcttgccatg gaacgggaac tgacggcgct ggaacaagac gccgccgtgc gcgtcatcgt
     2821 cttcaccggc gcgggcgaca aggccttcat cgccggcggc gacattgccg acctcaattc
     2881 gcgacagggc cttgcgcatt atctcgaatt cgccgaaatc gtccaccgcg tgttccgccg
     2941 catcgagctg tgcgacaagc cgaccatctg cgccatcaat ggctgggcgc tgggcggggg
     3001 caccgaactg ctgctcgcca ccgacatccg ccttgccgcc gaccacgcca ggcttggcct
     3061 gccggaaatc acgctcgggc tgtttccggg cgccggcggc acgcagcggc tgatccggca
     3121 ggtttcgccc tgcaaggcca aggagttgat gttcaccggt gaccgcatca ccgccaccga
     3181 ggcgctcgcg ctgggcctcg tcaaccgcgt ggtcgagaag gaaaggctgg ccgaggagac
     3241 gacggcgctg gccgaacgca tcgcgcagaa atcaccgctg gtgctgaaac tgctgaagcg
     3301 cacgctggcc agcggcgctg acatgcccct gccctcggcg ctggcgcatg aacaggcgat
     3361 gatcggcctc gtgctcgata cgcgcgacgc ccatgaaggc tgcgcggcct tcctcgaaaa
     3421 gcgccagccc gcctttgaag gcaggtagcg gcatggcgat cgacctcctg atgcccaagc
     3481 tcggcctgac catgacggaa ggcgtgcttt tggagtggaa ggccagcccc ggcgacaggg
     3541 ttgccaaggg ccagctgctc tatgtcgtcg agaccgacaa gatcgccacc gaagtcgaag
     3601 ccgaggccgc aggcgttctg gaagcgcaga tggtcggcga aggcgagacg gccgtcgtgg
     3661 gcgcggtgct tggaagactg gccatggagg gcgaggccgc cgcgcccaaa caggcagcgg
     3721 tcgccgcaac agccgcaccg cgcgcagcgg atgcgaagcc ggccaagctg gaagcactgg
     3781 aagccctcgc cctggcgcgc gcgccagccg gaagcgcaca gcgcattgtc gccacgccgc
     3841 tggcgcggcg catcgcgcag gccaatgccg tcgatctgca cggcgtcgcc ggcagcgggc
     3901 cgcgcggccg catcaaggcg atggacgtcg agcaggcgat gagcgcgcgc ccggcgcaag
     3961 cgtctggcgt cccggcgtct ggcatcccgg gaggcgtcac gcgctcgaaa ccttccgcca
     4021 cgcaggccgc gatggcgcgg cggctggccg aggtcaagca tggcgtgccg catttctatc
     4081 tttcgaccga gatcgaggtc tcggccctgc tggcgctgcg cgagacgctg aacgccgatg
     4141 cgcaggcgcc gcgcctgacg ctcaaccatt tcgtgctggc ggcgaccggg cgcgcgctgc
     4201 gcgaccatcc caacatcaac cgcgtctggg ccgatggcga actcgtcgcc ttcgccgaca
     4261 cggacgtttc gatggcggtc gagacggagc gcggcctgtt cgtgccggtg gtgcgcaatg
     4321 ccggtgcccg cccgctggac gcggtggccg ccgacgcccg tgctttggtg gcgcgggcgc
     4381 ggggcggcgg gctggccgcg gccgacatgg cgggcggggc gatcgcggtt tccaatgccg
     4441 gcatgcatga cgtgaccttc gtcacttcca tcatcaatcc cggccagtcc tccatcctcg
     4501 gcataggcag cgtgcgcgac ctgtttcgcc cggaccgcga aggaaagccc gaactgcgcc
     4561 gcgaaatggg cgtggtgttt tcgctcgacc accgcgtcca caccggcgtc gaaggcctgg
     4621 ccttcctttc cagcctgaaa gcctatctcg aaaccccgat gcggcttttg cgccaagtct
     4681 gatccaggaa ccacgatgac cgtcgaactc accgaagacc agaacatgat cgtcgccacc
     4741 gcgcggcggg tgggcgccga tttcgggctg gattactggc gcgacctcga tgcccgcaag
     4801 gcctatccga ccgaagcgtg gaaggcgatc tgcgatgccg gcctgtgcgg cgtggcgctg
     4861 ccggaggaat atggcggctc cgggcgcggc atgttcgaca tggcgctcat catcgaggcg
     4921 ctggcggcag gcggcgccgg ctcgaccatc gggcagctct tcatgtgcaa ccccatcttc
     4981 ggcggcgtgg ccatcgccaa atacggctcg gacgcgatgc ggtcgcagct gctgccgaag
     5041 ctgatttcgg gcgagctgca attctgcatg gcgctgaccg agccggatgc cggttccaac
     5101 acgctggaaa tgcgcgccta cgccgccccg gacggcgacg gcttccgcct caacgggcag
     5161 aagatctgga tcaccaacgt gcccgaaagc gacaagatgc tggtcgtggc gcgcaccata
     5221 aaatatggcg aaagcgagcg ccgcaccgac ggcatctcca tcttcctcat cgacacggac
     5281 cgcgccggca tcagccacgc caagatagac aagctcggca ccaacacgct cgcctcctcc
     5341 accgtcttct tcgaggatgt gcgcatcggg cgcgacgagg tgatcggcac gctgcacaag
     5401 ggctggccgg aactgctgga catcctcaac gccgagcgca tcgccaccac cgccggcctc
     5461 gtcggcacgg tcgatctcgc catcgggctg gccaccggct atgcgcgcga gcgcaagctg
     5521 ttcgccggca aacccatcgc cgcctatcag ggcctgcaat tcccgctggc gcaggcctat
     5581 gccgagaccg aatgcgcccg gctgatgaac cggcaggcgg ccaccctgtt cgacgagggc
     5641 aagccgttcg gcgtccagtc caacatcgcc aagctgatcg cagcgcaagc ctgctcggct
     5701 gccaccgagc gcgccatgca gaccatgggc ggcatgggat acgccaagga attccatgtc
     5761 gagcggctgt ggcgcgactg ccgcctgttc cgcttcgccc cggtgtcgga ggaaatgatc
     5821 ctcaactatc ttgcggtgca ccagctcggc atggtgcgct cctactgatt tgtccctcga
     5881 ccgccttctg ggaggaaacc gtggtcaatc tgagttcgtt cattcacttc cacgcgctgc
     5941 gccagccgga caagctcgcg ctggtctatg gcggcgagcg cgtcagctat gccgaactcg
     6001 accggcgcat cggggatacg gcggcgtacc tggccgcgca gggcatcggc cccggcgaca
     6061 tcgtggcggt gttcatgaag aacagcgccg ccttcatcga gatcgcctta gccaccagcc
     6121 atctgggcgc ggtgttcctg ccggtgaact tccgcctttc cggcgacgag tgcgcctata
     6181 tcgtggcgca tgccggcgcg aagcttctgc tggccgacgg tgaattcgcc gccacggtgg
     6241 ccggcatcgc caatgccgtg ctggtggacg aggcagcgca ggcctgtgcc ggcaggctgg
     6301 gcgcgccggg cttgccggcc cctgccctgc atctgcgccg gcccgaagac ctgttccggt
     6361 tgatgtacac ttccggcacc accgaccgcc ccaagggcgt gacgcacagc tacgagaatt
     6421 tctactggaa gtgcatggat catgtgatcg cgctcggcat gggcgaggcc gaccggctgc
     6481 tggctgtcgg gccgctctac catgtcggcg cgttcgacct gcccggcatc gccatattgt
     6541 gggttggcgg cacgctggtc gtgcagcgcc agttcgatga gacgcaggtg ctggccatca
     6601 tcgaggccga gcgcattacc tgcgcctggt tcgcgccgat gatggtgggc cgcatgctgg
     6661 cgctggccgg aacggacgcc tacgaccttt cctcgttcaa atggtgcatc gccggcggcg
     6721 agaagacgcc tgaaaaccgc atccgcgaat tcacgcgcct gttttccgcc gcccgcttca
     6781 tcgacggctt cggcctgacc gagacctgtt cgggcgacac gctgatggag gcgggcaagg
     6841 agatcgagaa gatcggctcc accggccgcg cgctcgccca tgtccagatc accatccgcg
     6901 acgatggtgg ccaggtgctg gcgcccggcg tcgagggcga gatctgcctg cgcggcccga
     6961 aggtgacgaa gggctactgg cgcgacgcca aaaagaccga gcagagcttc tatggcgact
     7021 ggttccgcag cggcgatgtc ggctatctgg acgaggacga cttcctgttc atcaccgacc
     7081 gcaagaagga catgatcatt tccggcggcg agaacatcgc ctcctccgag atcgaacgcg
     7141 tcatctacgc catgccggaa gtggccgaat gcgccgtcat cggcatgccg gacccgaaat
     7201 ggggcgagcg cccggcggcc atcatcgtgg tgcgcgaggg acacggcctt tccggcgagg
     7261 aagtccaggc gcgctgccgg gcggcgctgg cgagcttcaa ggtgccgacc cgaatattct
     7321 tccgcgacgc gctgccgcgc aacccttccg gcaaggtgct gaaacgggtg ctgcgcgacg
     7381 agaccgccgc cggcgacgac aaacaggagg ccaggccatg agtgccgata cccaaaccct
     7441 ctcgcagcgt atcggcgcct ttgtcgccgg cctttccatg gaaaccctgc cgggcggtgt
     7501 ggtggaaaag gcgcgcatct gccttttcaa cggctatggc atcgggcttg gctgccattc
     7561 cacgccctat gccgacgtgg cccgcgcagc tgcccttggt ttcgacggcg agcgcgccga
     7621 tggcgcaacc cttctgggcg acggccgcaa ggtggcggtc aacttcgccg cgatcgccaa
     7681 ctcggcgctg tttcatggcc gcgcgcagga agacacctgc ggcgcggccc atctcggcgc
     7741 gatcatgatc ccgctgctga ccgccctgct ggaggccagg aagctgcccg tggagcggct
     7801 tatcccggcg ctcgtcgccg gttatgaaac gggcggcctg ctggaaaaag cctattcgcc
     7861 gatcaccacg ccgcgcggcc tgcgttcctc gccgctctac ggcacgctgg cggcggccgc
     7921 cgctgccgcg cgcctgatgg gcctcgacgc cagccggacg gcggcggcca tcgactatgc
     7981 ggcctccttc accggcggca tcctgcaaag cttctcggaa ggcaccgacg agtggcgcta
     8041 ccaggtcggc gtcgccggcg cgacggggct gaccgccatg ctgctggccg ccaatggcgc
     8101 ggtatcggcc acccattcgc tggaaggccg cttcggcttt gcccgcacct ttgccgggac
     8161 cgattgcgac gtggacggcc ttgcctcgcg gctggggcgc gattgggcga tcgagcgcgt
     8221 gacgttcaaa ccctatccgg tctgcgcctt caaccagaca ccggtgatcg ccgcgctggc
     8281 gctgaaggaa aaggcggcgg ggcgcgactt cgccttgatc aaggtgcgga tgaacccgct
     8341 ggagaccggt tacgccggca tggattccac cggcccgttc cattccattt ccggcacgct
     8401 gatgagcatc cccttttgca ccgcgctgac gctggtgcgc ggcgagccga ccctgaagat
     8461 gatgacgacc tatgatgacg gcgcggtcaa cgcgctggtg tcgcgcgtcg agctcgtgcc
     8521 ggacgaaggc gtttcgcgcc tgtgctgcac catcgaggcc gagcttgccg gcggcgagcg
     8581 tatcggcatc gagcaggtga tgacgacgga gaacttcaac tacgaccgcg ccaccgtctc
     8641 gcagctggtg cgccgcatcg gcgcggagca ggacgttccg gccagggcct acgacctgat
     8701 cgaggacttc gcctttggcc tgccggacaa gggcgttggc caggttcttt ccgccttcgc
     8761 gctctgcccg cgcccggcat gaggcgcacc ggcgcatatc gccgcaaagc ggccgaatct
     8821 gctaaggctg ctttccagcc ggaggcacgg tccggcgccg aggagaaaat gagcgaaaag
     8881 cagcaggcaa tagtggttcg gcggccgcgc gggcaaaagc cccggcgcga ggaaaacgcg
     8941 gaagcgaaat tcaacgcgct gctacacgcg gcctgcgaga tcgtcggcga atatggctat
     9001 gtcgatgcca gcatttcgcg cattaccgag cgcgccggcg tggcgcaagg caccttctac
     9061 agctatttcg agagcaggca ggacctgttc gaccagctgc tgccgaagct tggcacggaa
     9121 atgctggcct tcatcaagga gcgcacgcgc ggtgtcgtcg acgcttcgga gcgggaacgc
     9181 aagggattca tggcgttctt cgatttcatc gtccagaacc cggatttcct gcgcattctc
     9241 aacgaagccg agcaactggc ccccaagggc catgaggcgc atttccagct ggtcgtcgcc
     9301 ggctatctcg ccaatctgca acgcgactgg gcgcgcggcg aatatccggg ctacgacgag
     9361 cgcgaactgg aagtgatcgt ctacatgctg ttgtcggcgc gatcctacct ggcgctgcgc
     9421 tatgccaagg acgaggacgg caccaggccg ttccccggct gggtcaccga cacctacatg
     9481 aagttcatca ccaacgggct gaaggcgaag ccctgacgtt caggcccggc ggctggagcg
     9541 gttctggttt tgaattggta gaaccgctct atctctttgt tttgacaaca ttttcggaca
     9601 aaaaacgttt cacgcttttg ctggaaatgc tctaccggaa caccttgtcg taggccttga
     9661 ctgccagttg cgccttgttg cgcacttctt cggcaatgtc gcccggcctg tacaggctgg
     9721 tgcccagccc gaacaggcgc acgccgaccc gtgcgaaatc ggagaaggtc agttccgaga
     9781 cgccgccgac cgcgcccacc acggcatcct tcggcaggat cgccttgatg gccgagatgc
     9841 cggatgcgcc cagcgcgcct gccgggaaga atttcagcgc cgacgcgccg cagcgcagcg
     9901 caagcaatgc ttcggtcggc gtgaagacgc cgggcagcga aaccatgccg caagcctgcg
     9961 ccctggcaat caccgccgga tcgacattgg ggctgaccat cagcctgccg ccggcatcgg
    10021 caacccgctg gcagtcttcg ggcgaaagca cggtgccagc gccgaccagc acatccttgc
    10081 cgaaagcctt cgccacgatc tcgatcgaac gcaacgcatc gggcgaattg agcggcactt
    10141 cgatggcctc gaacttttcc tgcgccaaaa cgccggcgat cgcctcgacc tcttggggcg
    10201 tcacgccgcg caggatggcg acgaggtcgc gccggaaggc cggccatgca atggcttcgc
    10261 tcatgcccta ctccttaccc caaagcctgg cggcggcttt agccaagcct atcctcaccg
    10321 cttcctcggc attggcaagt acgacattat agccggcctc taccaaagca gactgataga
    10381 gattgccgag cgcgcccgac gccagcagca cgatttcgcc cgctgcgccg taaagcgagc
    10441 gcgccgccgc gatctccgaa ccgatcaaaa ggccggacag gcgcggtgcg ccctgccgct
    10501 tttcctcgaa gccgagca
//
LOCUS       DASYCS010000142         2728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_610684,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000142 DASYCS010000000
VERSION     DASYCS010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610684"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1233
                     /locus_tag="VGM46_04755"
     CDS             <1..1233
                     /locus_tag="VGM46_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEY4191934.1"
                     /translation="LRAQEIALQNNLPCIYLVDSGGANLPNQDEVFPDRDHFGRIFFN
                     QATMSAAGIPQIACVMGSCTAGGAYVPAMADESIMVAKQATIFLGGPPLVKAATGEDV
                     TAEDLGGADLHTRLSGVADHLAQDDDHALAIVRRIVKNLNRSKAVGLVLLKPTAPLHA
                     QDEIYGIVPNDMRRPYDVREIIARIVDGSELDEFKQNYGTTLVTGFAHLHGMPVGIIA
                     NNGVLFSESALKGAHFIELCCQRKIPLVFLQNITGFMVGQKYEAGGIAKDGAKLVTAV
                     ATAQVPKVTMIIGGSFGAGNYGMCGRAFSPRFLWMWPNARISVMGGEQAAMVLAMVKR
                     EGIERKGETWSTEDEQKFKKPILMKYEHEGHPLYSSARLWDDGIIDPARSREVLALSL
                     SAALNAPIAETKFGVFRM"
     gene            1284..2036
                     /locus_tag="VGM46_04760"
     CDS             1284..2036
                     /locus_tag="VGM46_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191935.1"
                     /translation="MDSGHKVRVEAGVSFDWQALPRLVENLDGIDRNVERLHFRRGKS
                     STGGMAGFNRLKTLWLRQANQKVIDEISGLPLLDTLYIDGLSASSLKPLGEIRTLRRL
                     VIHGGAKVDNLDWIRGLPPGLLVLYLEGFKRISDLDAVKSLAQLTAFGFEGGMSTHAR
                     IDTLEPLASVSHLRYLFLAAARVATQSLEPLRRLPRLEHLECVMQFPEREFIELHKAR
                     PALHCDWFDMLENHGSLRAGKHAILDRIRHGG"
     gene            2064..>2728
                     /locus_tag="VGM46_04765"
     CDS             2064..>2728
                     /locus_tag="VGM46_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEY4191936.1"
                     /translation="MFKKILIANRGEIACRIIRTARRMGIATVAVYSDADANALHVAM
                     ADEAVHIGPSPVGQSYLVAEKIIEAARMRGAQAIHPGYGFLSENPAFVDEVIAAGLVF
                     IGPSAASIRAMGLKDAAKRLMEEAGVPVVPGYHGEAQEIVILASKAREIGYPVLIKAR
                     AGGGGKGMRRVDNPDDFAEALSGARREAKAAFGDDKVLVEKYVEKPRHIEVQVFGDNF
                     GNVV"
BASE COUNT          529 a          823 c          872 g          504 t
ORIGIN      
        1 ctgcgcgcgc aggagatcgc gttgcaaaac aacctgccct gcatctatct ggtcgattcc
       61 ggcggcgcga acctgcccaa ccaggacgag gtttttcccg accgcgacca tttcggccgc
      121 atcttcttca accaggccac catgtcggcc gccggcattc cacagatcgc ctgcgtcatg
      181 ggttcgtgca cggcgggcgg cgcctatgtg ccggcgatgg cggatgaatc catcatggtc
      241 gccaagcagg ccaccatctt tctcggtggg ccgccgctgg tgaaggcggc gaccggcgag
      301 gatgtcacgg cggaagacct tggcggggca gacctgcaca cgcgcctttc cggcgttgcc
      361 gaccatctgg cgcaggacga cgaccatgcg ctggccatcg tgcggcggat cgtgaaaaac
      421 ctcaatcgaa gcaaggcggt aggcttggtc ctactgaaac ctactgcgcc gcttcatgca
      481 caagatgaaa tctacggcat cgtgccgaac gacatgcgcc ggccctacga cgtgcgcgag
      541 atcatcgcgc gcatcgtcga cggttcggaa ctggacgagt tcaagcagaa ctacggcact
      601 acgctggtga ctggctttgc gcatctgcac ggcatgccgg tcggcatcat cgccaataat
      661 ggcgtgctgt tttccgaaag tgcgctgaag ggcgcgcatt tcatcgaact gtgttgccag
      721 cgcaagattc cgctggtttt cctgcaaaat atcaccggct tcatggtcgg gcagaaatac
      781 gaggccggcg gcatcgccaa ggacggcgcc aagctggtga cggcggtagc aaccgcccaa
      841 gtgccgaaag tgacgatgat catcggcggc tcgttcggcg cgggcaatta cggcatgtgc
      901 ggacgcgctt tctcgccgcg cttcctgtgg atgtggccga acgcccgcat ttcggtgatg
      961 ggcggcgaac aggcggcgat ggtgctggcc atggtcaagc gcgagggcat cgagcgcaag
     1021 ggcgagacgt ggagcaccga ggacgagcag aagttcaaga agccgatcct gatgaaatac
     1081 gagcatgagg gccatccgct ctattcctca gcgcggctgt gggatgacgg catcatcgat
     1141 ccggcaaggt cgcgcgaagt gctggcgctc agcctttcag ccgcgctgaa cgcaccgatc
     1201 gctgaaacga aattcggcgt gttcaggatg tgacggacat ttcaacgcgg aagcgacact
     1261 cagtcgagag aaatgaggca ggcttggata gcgggcacaa ggtgagggtc gaggccggcg
     1321 tcagtttcga ctggcaggct ttgccgcggc ttgtcgaaaa ccttgacggc atcgaccgaa
     1381 acgtcgagcg gctgcacttt cggcgcggaa agtcctccac cggcggtatg gcgggcttca
     1441 accgtctaaa gacgctctgg ctgcgacagg caaaccagaa ggtgatcgac gagatcagcg
     1501 gcttgccgct ccttgacacg ctttacattg acggtctttc ggcctcttcg ctcaagccgc
     1561 tgggcgaaat ccggacgctg cggcggctgg ttatccatgg cggcgccaag gtcgacaatc
     1621 tcgattggat acgcggcctg ccaccggggc tcctcgtgct ttaccttgaa gggttcaagc
     1681 gcatatccga tctcgacgcc gtcaaatccc tggcacagct taccgcgttc ggcttcgagg
     1741 gcggcatgag cacccatgcg cggatagaca cgctcgaacc gctcgcctcc gtctcgcacc
     1801 ttcgctacct cttcctggct gccgcgcgcg tggcgacaca gagcctggaa ccgcttcgcc
     1861 ggttgcccag gcttgagcat ttggaatgcg tcatgcagtt tccggagcgg gaattcatcg
     1921 agttgcacaa ggcgcggccc gcacttcatt gcgactggtt cgacatgctc gaaaaccacg
     1981 gaagcctgcg cgcaggcaag cacgctatcc tcgaccgtat ccggcatggt ggatgaaatg
     2041 aagcttcgac atcggaggat gccgtgttca aaaaaatcct gattgccaac cgtggtgaaa
     2101 tcgcctgccg gatcatacgc acggcgcgca ggatgggtat cgccacggtg gccgtctatt
     2161 cggatgccga tgcgaacgcg ctgcacgtcg cgatggccga cgaggccgtg catatcggtc
     2221 cctcgccggt tggccaaagc tatctcgtgg ctgaaaagat cattgaggcg gcgcggatgc
     2281 gcggcgcgca ggccatccac cccggctacg gcttcctgtc tgaaaatccg gcctttgtcg
     2341 atgaagtgat tgcggccggc ctcgtcttca tcgggccttc ggcggcttcc atccgcgcca
     2401 tggggctgaa agacgccgcc aagcggctga tggaggaagc cggcgtaccg gtggtccccg
     2461 gctatcacgg cgaggcgcag gagatcgtca ttctcgcctc caaggcgcgc gaaatcggct
     2521 atccggttct catcaaggcg cgcgccggcg gcggcggcaa gggcatgcgg cgggtggaca
     2581 accccgacga tttcgcggaa gcgctttccg gcgcgcggcg cgaggccaag gctgccttcg
     2641 gcgacgacaa ggtgctggtc gagaaatatg tcgaaaagcc ccgccacatc gaggttcagg
     2701 tgttcggcga caatttcggc aatgtcgt
//
LOCUS       DASYCS010000143         2619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1769865,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000143 DASYCS010000000
VERSION     DASYCS010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1769865"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VGM46_04770"
     CDS             complement(<1..206)
                     /locus_tag="VGM46_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006200777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 factor domain-containing protein"
                     /protein_id="HEY4191937.1"
                     /translation="MAQSLPSIVSGEGGLSRYLEEIRRFPMLQPQEEYMLGKRYAEHE
                     DTSAAHRLVTSHLRLVAKIAMGYRG"
     gene            complement(430..1500)
                     /locus_tag="VGM46_04775"
     CDS             complement(430..1500)
                     /locus_tag="VGM46_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEY4191938.1"
                     /translation="MSALSEQAHEMIEDDFDGDATVVAGADAAGQRLDQWLAGELGSA
                     MSRNRVQMLIRQGAVTVDGKPVGEGKRKMAAGERVSVVMPEPAPAEPQGEDIKLDVLF
                     EDDELIVIDKPAGLVVHPGAGNWTGTLVNALVHHCGASLSGIGGVRRPGIVHRLDKQT
                     SGVMVVAKTDAAHKALSEAFADHGRTGDLERAYAALVWGIPPRVKGTIDAPLGRAADR
                     VRRAVVPEQRDDARHAVTHFTVEERFGEEQKEFATASLVECRLETGRTHQIRVHMAHI
                     GHPVVGDPDYGRAFSTKANRLPEPLGLKVKGFPRQALHARLLAFRHPKTHIIMRFEAP
                     LPSDMQELVDGFRELGSGRPKS"
     gene            1576..1974
                     /locus_tag="VGM46_04780"
     CDS             1576..1974
                     /locus_tag="VGM46_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HEY4191939.1"
                     /translation="MARLQTEDAFADETAEKPLDPGIEKVRQRLVRFIAINLGLLFLA
                     LMVVIVALVYKSRSAKAPPPALASEIPGAAAEPVAGDIVLPVGARVVSQALSGNRLSI
                     DAELADGARAIFIYDIAERRMVGQFSIRNK"
     gene            2074..2148
                     /locus_tag="VGM46_04785"
     tRNA            2074..2148
                     /locus_tag="VGM46_04785"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2107..2109,aa:Glu,seq:ctc)
     gene            2506..>2619
                     /locus_tag="VGM46_04790"
     CDS             2506..>2619
                     /locus_tag="VGM46_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003498884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain containing protein"
                     /protein_id="HEY4191940.1"
                     /translation="MDHTKHIRLTTNELTPDILDDATIYGPGDEKVGSVSHV"
BASE COUNT          494 a          852 c          762 g          511 t
ORIGIN      
        1 ccgcgatagc ccatggcgat cttggcgacg agccgaaggt ggctggtgac gagcctgtgc
       61 gcggcggaag tgtcttcatg ctcggcgtaa cgcttgccga gcatatattc ttcctgcggc
      121 tgaagcatcg ggaagcggcg aatttcttcc aggtagcggc tgaggccgcc ttcaccggaa
      181 acgatactgg gtaatgactg ggccattgat gcgccctcct ctctcttgag aactgccccc
      241 taatcgcggc gggcatgtga cgcggatgcc gttaaggctc attcgcgtcg actttcatat
      301 acaggaacaa aaccagaaaa gacagctttt gttcagccct gaacagactg tcacgctgaa
      361 gtgaacaatg ccagtcaggt ttattgccga tcgattcaga atttttgtcg gttcaggttt
      421 attgccggtt caagattttg gccggccgga cccaagctcc cggaaaccat cgacaagttc
      481 ctgcatgtcg gatggaagcg gcgcctcgaa gcgcatgatg atatgggttt tgggatgccg
      541 gaatgcaaga aggcgggcat gcagggcttg ccggggaaag cctttgacct ttaggccgag
      601 aggttccgga agccggttgg ccttggtgct gaaggcgcgg ccataatcgg gatcgccgac
      661 gaccggatgg ccgatatgcg ccatgtggac gcggatctgg tgcgtgcgtc ccgtttccag
      721 ccggcattcg accaggctgg ccgtcgcaaa ttccttctgc tcttcgccaa aacgctcctc
      781 caccgtgaaa tgcgtcaccg catggcgggc gtcgtcgcgc tgctccggca ccacggcgcg
      841 gcgcaccctg tcggcggcac ggccaagcgg cgcatcgatg gtgcccttca cccgcggcgg
      901 gatgccccag acaagggcgg cgtaggcccg ctccaggtcg ccggtccggc catggtcggc
      961 gaacgcctcc gacaacgcct tgtgcgccgc atcggtcttg gccaccacca tcacgccgct
     1021 ggtctgcttg tcgagcctat gcacgatgcc cggccggcgc acgccgccaa tgcccgaaag
     1081 gctcgcaccg caatgatgaa ccagcgcatt gaccagcgtg ccggtccagt tgcccgcgcc
     1141 aggatgcacc accagcccgg ccggcttgtc gatgacgatc agttcgtcgt cctcaaacag
     1201 cacgtcgagc tttatatctt cgccttgcgg ttcggccggg gcgggttcgg gcatgaccac
     1261 cgaaacgcgc tcgcccgcag ccatcttgcg cttgccttcg ccgaccggct tgccgtcgac
     1321 ggtcaccgcg ccttgccgga tcagcatctg gacgcggttg cgcgacatag cggagccaag
     1381 ttcgccggcc agccattgat cgaggcgctg cccggcggca tcagccccgg ccaccaccgt
     1441 ggcgtcaccg tcaaaatcgt cctctatcat ttcatgggcc tgttcgctaa gagcgctcat
     1501 tgaaaacccc ttttgggaaa accctttttt gggaaacccg ttcgggaaaa cccttctctg
     1561 gtccggaact ttgccatggc ccggctgcaa accgaagacg cattcgccga tgaaacggcc
     1621 gaaaagccgc tcgatcccgg catcgagaaa gtacggcaga ggctggttcg cttcatcgcc
     1681 atcaatctcg ggctgctgtt tctcgccctt atggtggtga tcgtcgccct tgtctacaaa
     1741 tcgcgcagcg ccaaggcccc gccgcctgcc ctcgccagcg aaattccagg cgcggcggcc
     1801 gaacccgtgg ccggcgatat cgtcctgccc gtcggtgcca gggtggtgtc gcaggccctg
     1861 tcgggcaacc gcctgtccat cgatgccgag ctggccgatg gcgcaagagc gatcttcatc
     1921 tacgacatcg ccgaacgccg catggtcggc caattctcca tccgcaacaa atgacccggc
     1981 gcaccaaagc caaatgattt cgaccgccac attatcctgc gcaaggtcgg ttgctttttg
     2041 ccgagcgccg gcctatatcg ccgtctcttg agcgcgccca tcgtctagcg gtcaggacac
     2101 cgccctctca cggcgggaac aggggttcga ttccccttgg gcgtaccaga aatttcaaca
     2161 gcttggctgc gtatccgcga tttgccgccg atttcgtcga ggcagcaccg ctgctcgctg
     2221 gcctgaccgg ccgcctcgaa gccgttttca tccaacacat cgtcgcccgc caaacccggt
     2281 ttatcgacgg caaaaactcg acggcttcat gaggcggcgg ctggccatgc cgttccggct
     2341 tcaaccagca tcagttccgc cctcaaggga gcaggccacg gatcgcgatg cgttggaaat
     2401 cccgcgatca catttgtcat ttaccgggca tcgtgcggaa cggatggcct tgtcgggggt
     2461 tagcccagtc cagccaacaa gaaccagcca ccaaggagat gcgcaatgga ccacaccaag
     2521 catatacggc tcaccactaa cgaactgacg cctgacattc tcgacgatgc cacgatctac
     2581 ggccccggcg acgagaaagt gggttccgtc tcccatgtc
//
LOCUS       DASYCS010000144         5263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3220721,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000144 DASYCS010000000
VERSION     DASYCS010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 5263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5263
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3220721"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="VGM46_04795"
     CDS             complement(<1..684)
                     /locus_tag="VGM46_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY4191941.1"
                     /translation="MGAKGCERSFEALSTEAACARILERNRDAISVQKQHLAVANLAR
                     IIEATLKLSNKQGFHAMTLRELARESGLSMGGLYSYISHKEALLSMILREVSATVNDA
                     LSAPPAALRGNPAALTRWLIETHIRLTEAMQPWFVFAFMEAKSFPAPARKMAVDSERA
                     TEAIFEAAIRSGIDAGLFQVQDPELAASLIKPLIQDWYVKRSKYRKRGVSIERYIETV
                     TVFVEAALQA"
     gene            822..1577
                     /locus_tag="VGM46_04800"
     CDS             822..1577
                     /locus_tag="VGM46_04800"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018430912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEY4191942.1"
                     /translation="MPCLDLAGKTALITGASSGLGAHFANVLAACGVSVILGARRVEA
                     LENICKDIRARGGAARAIQMDVTQKISVERSFSEIEGSLDMVVNNAGVAANRGADDLD
                     EADWDAVLDTNLKGVFLVAQAAARHMKTVGSGGAIVNVASILGLRVAGGVSAYAASKA
                     GVIQLTKALALEWARHGIRVNALCPGYIETDLNRDFFSSQPGLAMIKRIPQRRLGKPE
                     DLDGALLLLCSDAGRYITGATLAVDGGHLVSSL"
     gene            1588..2841
                     /locus_tag="VGM46_04805"
     CDS             1588..2841
                     /locus_tag="VGM46_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY4191943.1"
                     /translation="MNFEIAPHIEDFRRRIASFVDREILPLESDRASYDAHENVALDL
                     LASLRARAKAEGLWCLQLKPATGGVGLGKVGMAVCYEEMNRSIFGPVVFNSAAPDDGN
                     MMVLEALGTPQQKETWLKPIVEGSVRSAFAMTEPHPGGGSDPSMIQTRAQKRGDIYVV
                     TGRKWFITGAAEASHFILMARTSDDPRHGLTAFLFHKDQPGWKIRRRIEIMGPEEHGG
                     HCELEFDGLEIPAENILLGEGKGLKVTQIRLGPARLTHCMRWLGLSKRCLEIARAYAA
                     ERQGFGQRLAERESIQLMLGDLAMRIEIGRLLVMKAAWELDRGGFARKEVSMAKIQVA
                     NVLHDAADTAIQINGARGYSKDTVLEWIYRYARQARLVDGADEVHKMVLNRFLNEEDR
                     QFWRWDVAKDDQPAPAGNALAGRTI"
     gene            2838..3551
                     /gene="fabG"
                     /locus_tag="VGM46_04810"
     CDS             2838..3551
                     /gene="fabG"
                     /locus_tag="VGM46_04810"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020010764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEY4191944.1"
                     /translation="MKPDVAIVTGAARGIGAAIAERLSRNGWRVAGFDRDFGEAPPSH
                     LAYWQVVDVADYEAVVTATREVEQNLGAVGAIVNNAGITRDAICHKMDPADFKAVLDV
                     NLSGPFHLCRALLGGMRERGFGRIVNISSMNAQRGQAGQSNYAAAKAGLIALTKSIAL
                     ENAAKGITANCIAPGFVQTDMTNAMRAEVREAEVARIPVGRIGTPADIASSVLFLMSD
                     DANFITGQVLSVNGGQFMP"
     gene            3594..5192
                     /locus_tag="VGM46_04815"
     CDS             3594..5192
                     /locus_tag="VGM46_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007067063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY4191945.1"
                     /translation="MQIENHLRPRAANHRPLTPLDFLERAVLSFPERPAVAWRETVWT
                     YREFHGIVARFAAFLVRQGVARGDVVSVMAQNRPEMLAAHYAVPMVGAVLNTINTRLD
                     TATVTYILEHAESRLVIADEACMQVAFEAAAAVGAPVFALADTDADKGDGRLHLLCGD
                     IAPAMPDVDAAITDEWQPICLNYTSGTTGQPKGVVYHHRGAYLNALGNVLALGLNQRS
                     VYLWTLPMFHCNGWCHTWAVTAAGGLHVCLEKIDPQLIFSSIERYGVTHMSCAPVVLY
                     MMVNHPSASPRKSGGRLTVVTGGASPTSALIEQIDRLHFDLIHLYGLTESYGPATLCI
                     LDEKDVGRDAPDKARLLARQGIRHATASRVQVIDKQGMEVAWDGSQPGEIVLRGNTLM
                     AGYYRDEAATEAAFADGVLHTGDIAVVHPDGYVEIKDRFKDVIISGGENISSLEIESV
                     LHQHPAVLLAAVVAVPDDKWGEIPFAFIELKPGASATAEELLQFARKHLAGFKVPRRF
                     AFRELPKTATGKIQKFVLRDVAQL"
BASE COUNT         1020 a         1609 c         1654 g          980 t
ORIGIN      
        1 cgcttgcagc gcggcttcaa cgaacacggt taccgtttcg atatagcgtt cgatgctgac
       61 gccgcgcttc cggtatttgc tgcgtttcac gtaccagtcc tggatcagcg gcttgatcag
      121 cgaagccgca agttcgggat cctgcacctg gaacagcccg gcgtcgatgc cgcttcggat
      181 agccgcctca aagatagcct cggttgctcg ttcgctgtcg accgccatct tacgggcagg
      241 ggcgggaaat gacttggctt ccatgaaggc gaagacaaac cagggctgca tggcttcggt
      301 cagccggata tgggtttcga tcagccaacg cgtgagagcc gccggattgc cgcgcaacgc
      361 ggcgggcggg gcgcttagcg cgtcgttcac cgtcgccgac acctcgcgca ggatcatcga
      421 caacaacgcc tctttgtgac tgatgtagga ataaagcccg cccatgctga ggccggattc
      481 cctggccagc tctcgcaagg tcatcgcatg gaacccctgc ttgttggaaa gtttcaaagt
      541 cgcctcgatg atgcgggcga gattggcaac cgcaaggtgc tgtttttgaa cgctgatggc
      601 gtcgcgattc cgttcaagga tgcgagcgca tgcagcctcg gtcgagagag cctcgaacga
      661 acgttcacaa cccttggcgc ccacgtcccc tccatgtgca atcgcctggc ccgaccattg
      721 gggcatccgc tggaagtagc agcaaattct tagcttgcaa gggtgcaccc atatccgtaa
      781 taaatcgagc gctcgttcgg attattatga atggatgtct catgccctgc ttggacctgg
      841 ccggcaagac ggcgctgata accggtgctt caagcggttt gggcgcgcat ttcgccaatg
      901 ttctggcggc ttgcggcgtc tcggtgatac tgggggcgcg ccgcgtggaa gcgttggaaa
      961 atatttgtaa agacattcgg gcgcggggtg gcgcggcgcg tgcgatccag atggatgtga
     1021 cgcagaagat atcggtcgaa cgctcgttct ccgaaatcga aggcagcctc gacatggtgg
     1081 tcaacaatgc cggcgtcgcc gcgaaccgtg gcgccgacga cctcgacgag gccgattggg
     1141 acgcggttct cgacaccaat ctgaagggtg tcttcctggt cgcccaggca gcggcccggc
     1201 acatgaagac ggtcggttcg ggcggtgcga tcgtcaacgt cgcgtccatt ctgggcctgc
     1261 gggtcgccgg cggcgtcagc gcctatgcgg cttcgaaagc cggcgtcatc cagctcacca
     1321 aggcgctggc gctcgaatgg gccaggcatg gcatccgcgt caacgctctg tgtcccggct
     1381 atatcgaaac cgatctcaac cgcgatttct tcagctcgca acctgggctg gcgatgatca
     1441 agcggatacc gcaacgccgc ctgggaaagc ccgaagacct cgacggcgcc ctgcttttgc
     1501 tatgctccga tgccggtcgc tacatcacgg gcgcaacgct cgccgtcgat ggcggacatc
     1561 tggtctcgtc gctctagggt gaaatgcatg aacttcgaaa tcgcgccgca catcgaggat
     1621 tttcgccggc gcatcgccag tttcgtcgac cgcgagatcc tgccgctgga gagcgaccgg
     1681 gcatcctatg atgcgcatga aaacgtcgcg ctcgatcttc tcgccagcct gcgggcgagg
     1741 gcgaaggccg aaggtttgtg gtgcctgcaa ctcaagcccg ccaccggggg cgtggggctt
     1801 ggcaaggtcg gcatggccgt gtgttacgag gaaatgaacc gctcgatctt cgggccggtc
     1861 gttttcaact cggccgcgcc cgatgacggc aacatgatgg tgttggaagc gctggggacg
     1921 ccgcaacaga aggaaacatg gctgaagccg atcgtcgagg gaagcgtccg ctcggccttt
     1981 gccatgaccg aaccgcatcc aggcggtggt tccgacccgt cgatgatcca gacgcgcgcg
     2041 caaaagcgtg gcgacatcta tgtcgtgacc ggtcgcaaat ggttcattac cggcgcggcg
     2101 gaggcgagcc atttcatctt gatggcgcgc acatccgacg atcctcgcca tggcttgacc
     2161 gcgttcctct tccacaagga tcaaccggga tggaagatcc ggcgcaggat cgagatcatg
     2221 gggccggaag aacatggcgg ccattgcgaa cttgaattcg atggtctcga aattcctgcc
     2281 gaaaacatcc tgcttggcga gggcaaaggg ctgaaggtca cgcagatacg tctgggtccg
     2341 gcccggctga cgcattgcat gcgctggctc ggcctctcaa aacgctgcct tgagatagca
     2401 cgcgcctacg ccgccgaacg tcagggcttt ggccagaggc tggccgagcg cgaaagcatc
     2461 cagctcatgc tgggcgacct cgccatgcgc atcgagatcg gacgcctgct ggtgatgaag
     2521 gccgcctggg aactcgaccg tggcggcttt gcccgcaagg aggtgtcgat ggccaagata
     2581 caggtggcga acgtgctgca tgacgccgcc gacacggcga tacagatcaa cggcgcgcgc
     2641 ggctattcga aagacaccgt tctggaatgg atttatcgct atgcccgtca ggcgcgtctc
     2701 gttgacggcg ccgacgaggt ccacaagatg gtgctcaacc gcttcctcaa cgaggaagat
     2761 cggcagttct ggcgctggga cgtggcaaaa gacgatcagc cggcgccggc tggaaatgct
     2821 cttgccggga ggacaatatg aagccggacg ttgccatcgt gacgggtgct gcgcgcggta
     2881 tcggcgccgc catcgccgag cgcctttccc gcaacggctg gcgcgtggcg ggttttgacc
     2941 gcgacttcgg cgaggccccg ccctcgcacc ttgcatactg gcaggtcgtg gacgttgccg
     3001 actacgaggc ggtggtgacg gcgacgcgcg aggtcgagca aaatctcggc gcggttggag
     3061 ccatcgtgaa caatgccggc atcacgcggg atgccatttg ccacaaaatg gaccccgccg
     3121 atttcaaggc ggtgctggat gtcaatcttt caggcccgtt tcacctgtgc cgcgcccttc
     3181 ttggcggcat gcgcgagcgc ggtttcggcc ggatcgtcaa catctcgtcg atgaacgcgc
     3241 agcgaggtca agccgggcag tccaattacg cggccgccaa ggctggactg atcgccctga
     3301 cgaagtcgat cgcacttgaa aacgccgcca agggaataac cgccaactgc atcgcgcctg
     3361 gcttcgtcca gaccgacatg accaatgcga tgcgcgccga ggtgcgtgag gccgaggtgg
     3421 cgcgcatccc cgtcggcagg atcgggacgc cagccgacat cgcgtcgtct gtcctgttcc
     3481 tgatgtccga cgatgcgaat ttcatcaccg gccaggtgct ctcggtaaat ggcgggcagt
     3541 tcatgccttg atctggaaaa ccacaggcaa ccgtttttcc ggggaggata aggatgcaga
     3601 tcgaaaacca tctcaggcca cgcgcagcca accatcgtcc gttgacgccg ctggatttcc
     3661 tggaacgggc cgtgctgagc ttcccggaac ggccggcggt tgcctggcgc gagacggtgt
     3721 ggacctatcg ggaattccac ggaattgtcg cccggttcgc ggcgtttctt gttcgccaag
     3781 gcgtggcgcg cggcgatgtc gtctcggtca tggcgcaaaa ccgcccggaa atgctggctg
     3841 cccactacgc cgtaccgatg gtcggcgcgg tgctgaacac catcaacacc aggctcgaca
     3901 cggccaccgt cacctacata ctcgaacatg ccgaaagccg cctggtcatc gccgacgagg
     3961 catgcatgca ggtggcgttt gaagcggcgg ctgcggtagg agcgcccgtc ttcgcgctcg
     4021 ccgacacgga tgccgacaag ggcgacgggc ggctccatct gctttgcggc gacatcgcgc
     4081 cggccatgcc cgatgtcgat gcggcaatca ccgacgagtg gcagccgata tgccttaact
     4141 acacgtctgg aacgaccggc cagcccaagg gcgtcgtcta ccatcacagg ggtgcctatc
     4201 ttaacgcgct cggaaacgta ctggcgctcg gactgaacca gcgatcggtc tatctttgga
     4261 ccctgccgat gttccactgc aacggctggt gccacacatg ggccgtcacg gctgccggcg
     4321 gcctgcatgt atgccttgag aagatcgatc cccaactcat cttctcctcg atcgaacgct
     4381 atggcgttac tcatatgagc tgcgcgcccg tggtgctcta tatgatggtc aatcatccga
     4441 gcgcctcgcc caggaaatcc ggcggcaggc tgaccgtcgt gacgggcggg gcctcgccaa
     4501 ccagcgcgct gatcgagcag atcgacaggc tccacttcga tctgatccat ctctacgggc
     4561 tgaccgaatc ctacgggccg gcaacattgt gcattctcga tgaaaaggac gtcggcaggg
     4621 atgcgccgga caaggcgcgg cttcttgcgc gccagggtat caggcacgcc acggcaagcc
     4681 gcgttcaggt gatcgacaag caaggcatgg aggttgcctg ggacggcagc cagcccggcg
     4741 aaatcgtctt gcgcggtaat acgctgatgg caggctacta ccgcgacgag gccgccaccg
     4801 aggccgcctt cgccgacggc gtattgcata ccggcgacat agccgtcgtt catcccgacg
     4861 gctatgtcga aatcaaggat cgcttcaagg acgtgatcat ctcgggtggc gaaaacatct
     4921 ccagcctgga aatcgaaagc gtgctgcacc agcatccagc cgttcttctg gccgcggtgg
     4981 tggcggttcc cgacgataaa tggggcgaaa tcccattcgc cttcatcgaa ctgaagcccg
     5041 gcgcatcggc gaccgccgag gaactcctgc aattcgcccg caagcacctg gccggtttca
     5101 aggtcccgcg ccgcttcgcg ttccgggaac ttcccaaaac ggcaaccggc aagattcaga
     5161 aattcgttct gcgcgatgtg gcacagctct gaccaccggc cgcaaagcgg cattcaggag
     5221 aggcacgccc ttggcagatc gtagcgacgc gcaaccggaa atg
//
LOCUS       DASYCS010000145         1554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_319880,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000145 DASYCS010000000
VERSION     DASYCS010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319880"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1554
                     /locus_tag="VGM46_04820"
     CDS             <1..>1554
                     /locus_tag="VGM46_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEY4191946.1"
                     /translation="GAKITVRSLRKLQEDGVKNIAFAQDELIGRFSALDMVNPETGEI
                     YVEAGDELTADTLATLAEAGFSEVEVINVDSITTGAYVRNTLAIDKNHSREQALIDIY
                     RVMRPGEPPTLDTAETLFGQLFFDPERYDLSAVGRVKMNMRMGLDAPDTMRVLRKEDI
                     LAILKALAELRDGRGEIDDIDNLGNRRVRSVGELMENQFRVGLLRMERAIKERMSAVD
                     IDTVMPHDLINAKPVAAAVREFFGSSQLSQFMDQQNPLSELTHKRRMSALGPGGLTRE
                     RAGFEVRDVHPTHYGRICPIETPEGPNIGLINSLATFARVNKYGFIESPYRKVVNKHL
                     TDEVIYLSAMEEAKFMIAQANAAVDSRLRLTDELVSSRKGGNFVMTTPDQVQFIDVSP
                     KQLVSVAAALVPFLENDDANRALMGSNMQRQAVPLLRPEAPLVGTGMEEVVARDSGAA
                     ITARRSGIVDQVDATRIVVRATEEADPTKPGIDIYRLRKFQRSNASTCITQKPLVKVG
                     DLLKKGDIIA"
BASE COUNT          320 a          504 c          461 g          269 t
ORIGIN      
        1 ggcgccaaga tcaccgtgcg gtccttgcgc aagctgcagg aagacggcgt caagaatatc
       61 gccttcgccc aggatgaact gatcggccgc ttcagcgcgc tcgacatggt caatccggaa
      121 accggcgaaa tctatgtcga agccggtgac gaactcaccg ccgacacgct cgccaccctg
      181 gcggaagccg gtttcagcga agtggaagtc atcaacgtcg acagcatcac caccggcgcc
      241 tatgtccgca acacattggc gatcgacaag aatcattccc gcgaacaggc cctgatcgat
      301 atctaccgcg tcatgcgtcc cggcgagccg ccgaccctgg acaccgccga aaccctgttc
      361 ggccagctct tcttcgatcc ggaacgctat gacctgtcgg cggtcggccg ggtgaaaatg
      421 aatatgcgca tggggctgga tgctcccgac accatgcgtg tgctgcgcaa ggaagacatt
      481 ctcgccatcc tcaaggccct cgcggaactg cgcgatggcc gcggcgaaat cgacgacatc
      541 gacaatctcg gcaaccgccg cgtgcgttcg gtgggcgagc tgatggaaaa ccagttccgc
      601 gtcggcctgc tgcgcatgga gcgcgccatc aaggagcgca tgagcgcggt ggatatcgac
      661 accgtgatgc cgcacgacct gatcaacgcc aagccggtcg cggctgcggt gcgtgaattc
      721 ttcggctctt cgcagctgtc gcaattcatg gaccagcaga accccctgtc ggaactgacc
      781 cacaagcgcc gcatgtcggc gcttggaccg ggcggcctga cccgcgagcg cgccggcttc
      841 gaagtgcgcg acgtgcaccc gacccattat ggccgtatct gcccgatcga aacgccggaa
      901 ggcccgaaca ttggcctgat caattcgctg gcgaccttcg cccgcgtcaa caaatacggc
      961 tttatcgaaa gtccctaccg caaggtggtg aacaagcatc tgacggatga ggtgatctat
     1021 ctctcggcga tggaagaggc gaagttcatg atcgcccagg ccaatgccgc ggtggacagc
     1081 cgcctgcgcc tgaccgacga acttgtgtca tcgcgcaagg gtggaaactt tgtgatgacc
     1141 acgcccgacc aggtacagtt catcgacgtt tcgcccaagc aactggtttc cgtcgccgcc
     1201 gcactggtgc cgttcctgga aaatgacgac gccaaccgcg ccctgatggg ttcgaatatg
     1261 cagcgtcagg ccgtgccgtt gctgcgcccc gaagcgccgc tggtcggcac cggcatggag
     1321 gaagtggtgg cccgggactc cggcgccgcc atcaccgccc gccgttccgg catcgtggat
     1381 caggttgatg ccacccgtat cgtggtgcgt gccaccgaag aagccgatcc caccaaaccg
     1441 ggcatcgaca tttatcgtct gcgcaagttc cagcgctcca acgcctcgac ctgcatcacg
     1501 caaaagccgt tggtgaaggt gggcgatctg ctcaagaagg gcgatatcat agcc
//
LOCUS       DASYCS010000146         4389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4235692,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000146 DASYCS010000000
VERSION     DASYCS010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4389
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4235692"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..794)
                     /locus_tag="VGM46_04825"
     CDS             complement(81..794)
                     /locus_tag="VGM46_04825"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006204995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY4191947.1"
                     /translation="MGFDLPRDIVLPVETIDVRLDPAPHPLERDNLAAIEENWRHEVS
                     INRHLFDGQVALLSELSYNEGRLVGRCHIVRYATFMYWLRNLETPGAGHVFAHAMLVS
                     SDNKLVAIRMGKHTVNAGRVYFAAGSFELEDFHDGLVDVDFNMKREVKEETGLDIAEA
                     SPAKRYYLLSTRSGTVIFRRYRMPLTANAMVSRIKAFVAAEANPEISAPVVIHSASDL
                     PDDLAGHMKPLIQWHFSGA"
     gene            complement(805..1917)
                     /locus_tag="VGM46_04830"
     CDS             complement(805..1917)
                     /locus_tag="VGM46_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006204994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEY4191948.1"
                     /translation="MSLRLATFNVENLMNRFDFSGYRNQLNQDRTLSLFDIGSEAEYR
                     SLEQARAIAQSDDTRQLSALAIAATRADMICMQEVDNIEALKAFEYGYLFKMIGEGYR
                     QKYTTAGNDSRGIDVAVMMRNETADGQPIEFVRMTSHAHVTYDDFGLHTSELEATGNA
                     ANERIFRRDCLEIDVTVAGKPLTLYLVHFKSMGPPRNGLDGRQASMPIRIAEAQAVRR
                     IIEERFGKDGAAAKNWAICGDMNDYRQRVKIDGEPLAGYRFEVVDENRSCLDVLLAGG
                     FCENVVERRPEMDRWSFYHTRGPQERHLCQLDYILLSKGLAARNAAAVPDIVRNGQPW
                     RTVFPPGQEVERFPRAGWDRPKASDHCPVAITLNIS"
     gene            2082..2603
                     /locus_tag="VGM46_04835"
     CDS             2082..2603
                     /locus_tag="VGM46_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013894784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BA14K family protein"
                     /protein_id="HEY4191949.1"
                     /translation="MKPLFFRSMKTGLMALGLVAGLPGAAFAGPTTLPAPSMSPNMSA
                     REIMPVRDSWAGGNSRGNYWRHGQNRHWRGNNWRGDRWNHNGWHRPSHDGWRWRSYRR
                     HYGGSGIYLGLGGLAIAPAYDYYAPRRAYRVYRGSGAHVRWCYNRYRSYRASDNTFQP
                     YNGRRQQCYSPYR"
     gene            complement(2673..3692)
                     /gene="ilvC"
                     /locus_tag="VGM46_04840"
     CDS             complement(2673..3692)
                     /gene="ilvC"
                     /locus_tag="VGM46_04840"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018324362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEY4191950.1"
                     /translation="MRVYYDRDADLNLIKGKKVAIIGYGSQGRAHALNLKDSGVKEIA
                     IGLKAGSATAKKVEADGLKVMSVADAAKWADLMMMATPDELQADIYKNDIAPNIRDGA
                     AIAFAHGLNVHFGLIEPKSTIDVVMIAPKGPGHTVRGEYQKGGGVPCLVAVNHDASGN
                     AHDLALSYACGVGGGRSGVIETNFKEECETDLFGEQVVLCGGLVELIRAGFETLVEGG
                     YAPEMAYFECLHEVKLIVDLIYEGGIANMNYSISNTAEWGEYVSGPRIITADTKAEMK
                     RVLKDIQTGKFTSEWMQEYRAGLSRFKGIRRNNDSHQIEEVGAKLRAMMPWIAKNQLV
                     DKAKN"
     gene            complement(3719..4387)
                     /locus_tag="VGM46_04845"
     CDS             complement(3719..4387)
                     /locus_tag="VGM46_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015317311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY4191951.1"
                     /translation="MAGAELLQKAPSQDDGKELSERQQTVLDAALRLLVEDGDNLTMT
                     AVARRASCSKETLYKWFGDRDGLLTATVQWQASKVRVAALGRQGLDLASLTASLEGFA
                     SDWLKVISSDTSIALNRVAISHAGSGKDNLGAIVLEKGRFALASRLKPVLEAGKEAGF
                     LDFPDAETAFRTFFGLVARDVQIRALLGDRLELTAASIGGDAVRATQQFLALYGQNRL
                     VEPA"
BASE COUNT          823 a         1377 c         1298 g          891 t
ORIGIN      
        1 tgttcggttt tttcccggcg ctgcgcggcg caaggctaaa ccccatcgac gcgctgcggc
       61 acgaatagcc gcaggttccg tcaggccccg gagaaatgcc actggatcaa cggcttcatg
      121 tgcccggcga gatcgtcggg caggtcgctg gcgctgtgga tgacaacggg cgcggatatc
      181 tcgggattgg cctcggcggc cacgaacgcc ttgatgcggc tgaccatcgc attggccgta
      241 agcggcatgc ggtagcggcg gaaaatcacc gtgccgctgc gcgtcgacaa caggtaatag
      301 cgcttggccg gcgaagcctc tgcgatatcg aggccggtct cctccttgac ctcgcgcttc
      361 atgttgaagt cgacatcgac cagcccgtca tgaaaatcct ccagttcgaa ggaaccggcg
      421 gcgaaataca cgcgccccgc attgaccgtg tgcttgccca tgcggatcgc caccagtttg
      481 ttgtcgctgg aaaccagcat ggcgtgggcg aaaacgtgac cggcgcccgg cgtctccaga
      541 tttcggagcc agtacatgaa agtggcgtag cgcacgatat ggcaacgccc cacaagtcgg
      601 ccctcattgt atgaaagctc ggaaagcagc gccacctggc catcaaacag gtgacggttg
      661 attgaaactt cgtggcgcca gttttcctcg atcgctgcaa gattgtctct ttccagcgga
      721 tgaggcgcgg gatcgagcct tacgtctatg gtctcgacgg gcaggacgat atcgcgcggc
      781 agatcgaaac ccatgccaaa tccttcagct aatattcaac gtgatcgcca ccggacaatg
      841 atccgatgcc ttgggccggt cccagcccgc tcgcggaaac cgctcgacct cctggcccgg
      901 cgggaagacg gtgcgccagg gctggccgtt gcggacgatg tctggaacgg cggccgcatt
      961 gcgcgccgcc agtcccttcg acagcaggat ataatcaagc tggcacaaat gtcgctcctg
     1021 cggaccgcgc gtatggtaaa acgaccaacg gtccatttcc ggcctgcgct ccactacgtt
     1081 ttcacaaaag ccaccggcca gaagaacgtc aaggcaagat cgattctcgt ccaccacctc
     1141 gaaacgataa ccggccagcg gctcgccgtc gatcttcacg cgctgccgat aatcgttcat
     1201 gtcgccgcag atggcccagt tcttcgcagc cgctccatcc ttgccgaagc gctcttctat
     1261 gatccgcctc acggcttgcg cctcggcgat ccggatcggc atgcttgcct gccgcccatc
     1321 cagcccgttg cgcggcggcc ccatcgactt gaaatgaacg agatagagcg tcagcggctt
     1381 tccagcgacc gtcacatcga tctccaggca atcacgccgg aagatgcgct cgttggcggc
     1441 gttgccggtc gcttcgagtt cggacgtatg caggccgaaa tcgtcatagg tgacatgggc
     1501 gtggctcgtc atgcgcacga actcgatcgg ctggccgtcc gccgtttcat tgcgcatcat
     1561 caccgccacg tcgatgccgc gtgaatcatt gccggccgtg gtgtattttt gccggtagcc
     1621 ttcaccgatc atcttgaaaa gatagccata ttcgaaggcc ttcagcgctt cgatattatc
     1681 gacttcctgc atgcagatca tgtcggcgcg cgtcgcggcg atggcgagcg ccgacagctg
     1741 ccttgtatcg tccgactgcg cgatcgccct tgcctgttcg agcgagcggt attccgcctc
     1801 gctgccaatg tcgaacagcg acagcgtgcg gtcctgattg agttggtttc gatagccgga
     1861 gaaatcgaac ctgttcatca ggttttcgac attgaaagtg gcaaggcgca gcgacatggg
     1921 catgaggttt gcccggaagc agcttcgaaa acaagggtct gcggccatag attggagcaa
     1981 ggcggaccgg aacttttccg ttcatcctcc gttcagggtc aaacggccag catactgccc
     2041 attctcggga tccgtggcgc gggggccaaa ggattttgga aatgaaacca ctcttttttc
     2101 gctcgatgaa aacagggctt atggcgcttg gcctggtggc cggcctgccc ggcgcagcct
     2161 tcgccggtcc gacgaccctg ccagcgccgt ccatgtcgcc caacatgagc gcgcgggaaa
     2221 taatgccggt gcgtgatagc tgggccggcg gcaacagccg tggaaattac tggcgacacg
     2281 gacaaaaccg ccattggcgc ggcaataatt ggcgaggaga ccgctggaac cacaatggtt
     2341 ggcatcgccc ctcccacgat ggctggcgct ggcgcagcta caggcggcac tatggcggtt
     2401 cgggcatcta tctggggctt ggcggcctcg ctatagcacc cgcctatgac tattacgcgc
     2461 cacgccgggc ttaccgcgtc tatcgcggtt ccggcgcgca tgttcgctgg tgctataatc
     2521 gctaccgttc gtatcgcgct tccgacaata cgttccagcc ctacaatggc cggcggcagc
     2581 aatgttattc gccctaccgc tgaaccgggc gcaaaaaaag ccggcgtttc gcgccggctt
     2641 tttccattcg atgactgaaa gcgaaaaacg ccttagttct tggccttgtc caccagctgg
     2701 ttcttggcga tccacggcat catcgcacgc agctttgcgc cgacttcctc gatctggtgg
     2761 ctgtcgttgt tgcggcggat gcccttgaag cgcgacaggc cggcgcggta ttcctgcatc
     2821 cattccgagg tgaacttgcc ggtctggatg tccttcagca cgcgcttcat ctcggccttg
     2881 gtgtcggcgg tgatgatgcg cgggccggag acatattcgc cccattcagc cgtgttcgag
     2941 atcgaatagt tcatgttggc gatgccgcct tcatagatca ggtcgacgat cagcttcact
     3001 tcgtgcaggc attcgaaata agccatttcc ggcgcatagc cgccttccac cagcgtctcg
     3061 aagccggcgc ggatgagttc gaccaggccg ccgcacagca ccacctgctc gccgaacaga
     3121 tcggtttcgc actcttcctt gaagttggtt tcgatgacgc ccgaacggcc gccgccaacg
     3181 ccgcaggcat aagacagcgc cagatcgtgc gcattgccgg aagcatcgtg attgacggcg
     3241 acgaggcacg gcacgccgcc gcccttctgg tattcgccgc gcaccgtatg gccggggcct
     3301 ttcggcgcga tcatgacgac gtcgatggtc gacttcggct cgatcaggcc gaaatgcacg
     3361 ttgaggccgt gcgcgaaggc aatcgccgcg ccgtcgcgga tgttgggcgc aatgtcgttc
     3421 ttgtagatgt cggcctgcaa ttcgtcaggc gtcgccatca tcatcaggtc ggcccacttg
     3481 gcggcatcgg ccacgctcat caccttgagg ccgtccgctt ccaccttctt ggcggtcgcc
     3541 gaaccggcct tcaggccgat ggcgatttcc ttgacgccgg aatccttcag gttgagcgca
     3601 tgtgccctgc cctggctgcc gtaaccgatg atggcgacct tcttgccctt gatcagattg
     3661 agatcggcat cgcgatcgta atagacacgc attttgttct tccttcccgt ttgaacgatc
     3721 aggccggttc caccagccgg ttttgcccat aaagagccag aaactgctgt gtcgcgcgca
     3781 cggcgtcgcc gccgatggac gcagcagtca attccagccg gtcgccgagc aacgcgcgga
     3841 tctgcacgtc gcgggcgacc agcccgaaaa atgtccggaa tgccgtttcg gcgtccggga
     3901 agtccagaaa gccagcctct ttgccggcct ccagaaccgg cttcaatcgg ctggccagcg
     3961 caaagcgccc tttttccagc acgatggcgc caaggttgtc cttgcccgaa ccggcatggc
     4021 tgatggctac gcggttaagc gcgatcgagg tgtcgctgga aatgaccttg agccagtcgg
     4081 aggcaaagcc ttcgaggctg gcagtcagcg aagcgagatc gaggccctgg cggccaagcg
     4141 cggcgacgcg caccttggat gcctgccatt gcacggtagc ggtcagcagg ccgtcgcggt
     4201 caccgaacca tttgtagagc gtttccttgg agcagctggc gcgccgagcc accgcggtca
     4261 tggtcagatt atcgccgtcc tccaccaaaa ggcgcagcgc cgcgtcgaga accgtctgct
     4321 ggcgctcgct cagttccttg ccatcgtcct gggaaggcgc tttctgcaac aattcggctc
     4381 cggcaatcc
//
LOCUS       DASYCS010000147         2191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4957712,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000147 DASYCS010000000
VERSION     DASYCS010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4957712"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..955)
                     /gene="corA"
                     /locus_tag="VGM46_04850"
     CDS             complement(<1..955)
                     /gene="corA"
                     /locus_tag="VGM46_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HEY4191952.1"
                     /translation="MKRMKVKRRRTPVGASPGTLIADPAASRSELCLTLISPEKCKTI
                     RHASIADVAAHCDKWQTLWLDCTGLGSIELIQEIGRLFGLHPLALEDVVNTGQRPKVD
                     FFDDHAFVVLSMIDDVATSRYEQVAVFFGKKFVITFQERAGDPFGPVRKRIESPMPNR
                     LRSRGSDYLAYALIDAIVDSYFPPVEAASETVDHIEDEMMTAPQKQQMRQLHELRRGT
                     SVLKGVLWPLRDALAGLIRSEAAYVAAETKIFLNDTLDHAMRLIELVETQRDTVTGLM
                     EMHLSLSQARTNDVIGYLTIVSVIFMPLTFLVGVWGMNFDPD"
     gene            complement(1098..2099)
                     /gene="truB"
                     /locus_tag="VGM46_04855"
     CDS             complement(1098..2099)
                     /gene="truB"
                     /locus_tag="VGM46_04855"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019863076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEY4191953.1"
                     /translation="MARRGKKKGRPISGWVVLDKPVGMGSTEAVSKVKWLFQAEKAGH
                     AGTLDPLASGMLPIALGDATKTVPYVQDGAKIYRFTVAWGQERSTDDLEGPVTKTSDT
                     RPSQADVAAILQNYTGVIMQTPPQFSAIKIAGERAYDLARSGAEVDIPAREVEIGRLE
                     IIEHLADRTLFETECGKGTYVRSLARDMGRDLGCFGHIADLRRMEVDPFTAEDFVTIA
                     ELEAARFGEQPQGEAGEDAGQAGEDIEHAEPVAPPARFDEIDALLVDTGAALDCLPQV
                     AVSDDAATRIRLGNPVVIRGRDAPVEAEEACATVRGKLVAIGAIEQGMFKPKRVFGG"
     gene            complement(2099..>2191)
                     /locus_tag="VGM46_04860"
     CDS             complement(2099..>2191)
                     /locus_tag="VGM46_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013891266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HEY4191954.1"
                     /translation="FDNFAKINDLLKSPEVARDLGQDEDGEDKE"
BASE COUNT          395 a          702 c          674 g          420 t
ORIGIN      
        1 tatcggggtc gaaattcatg ccccacacgc cgaccagaaa ggtcagcggc atgaagatca
       61 ccgaaacgat agtgaggtag ccgatgacgt cattggtgcg cgcctgcgac aaggacaggt
      121 gcatctccat caggccggtg acggtgtcgc gctgggtttc aaccagttcg atcagccgca
      181 tggcatggtc gagtgtatcg ttcaggaaga ttttcgtttc ggccgcgaca taggccgcct
      241 cggaacggat caggccggca agcgcgtcac gcaacggcca aagcacgcct ttcaggacgc
      301 tggtgccacg gcgcaactca tgaagctggc gcatttgctg cttctgcggc gcggtcatca
      361 tctcgtcttc gatatggtcg accgtctcgc tggcggcctc caccggcggg aaatagctgt
      421 cgacgatggc gtcgatcagc gcgtaggcca gataatccga accgcgcgag cgcaggcggt
      481 tcggcatcgg gctttcgatg cgtttgcgca ccgggccgaa tggatcgccc gcgcgctcct
      541 ggaacgtgat gacgaatttc ttgccgaaga agacggcgac ctgctcgtag cggctggtgg
      601 cgacatcgtc gatcatcgac agcacgacaa aggcatggtc atcgaagaag tcgacctttg
      661 gccgctgccc ggtattgacc acatcttcca gcgccagcgg gtgcaggcca aacaggcggc
      721 cgatttcctg gatcagctcg atgctgccaa gccccgtgca atccagccag agcgtttgcc
      781 atttatcgca atgggcggcc acatcggcga tgctggcatg gcggatggtc ttgcattttt
      841 cgggcgaaat cagcgtcagg caaagctcgc tgcggctggc ggccggatcg gcaatcagcg
      901 tgccgggcga tgcgccaacc ggcgtgcgcc gtctcttcac cttcatgcgc ttcataggtc
      961 ggccgcctcc gccagtgccg gtccgaagcg tgccacggtc cttcgggtcg cctgccacgc
     1021 tttgtctcat cgcatgtcgt ggcaaaagcg cgccacgaca tgcttatggg aggcagcccg
     1081 ccgggcctgt caagggatca accgccaaag acgcgctttg gcttgaacat gccttgttcg
     1141 atcgcgccga tggcgaccag cttgccgcga acggtggcgc aggcttcctc ggcttcgacc
     1201 ggcgcatcgc ggccacggat cacgaccgga ttgccaagcc tgatcctggt tgccgcatcg
     1261 tcgctgacgg caacctgcgg caggcaatcc aatgctgcgc ccgtatcgac aagcagcgca
     1321 tcgatctcat cgaaacgagc cggcggggcg acaggctccg catgctcgat atcctcgccc
     1381 gcctgcccgg catcctcgcc tgcctcgcct tgcggctgtt cgccaaaacg cgcggcttcc
     1441 agttcggcga tggtgacgaa atcctccgcg gtgaacggat cgacttccat gcggcgcaaa
     1501 tcggcaatgt ggccgaagca gcccagatca cggcccatgt cgcgggccag cgagcgcaca
     1561 taggtgccct tgccgcattc ggtctcgaaa agcgtgcggt cggccaggtg ctcgataatt
     1621 tcaagccggc cgatttccac ttcgcgcgcg ggtatgtcga cttcagcgcc gctgcgggca
     1681 aggtcatagg cgcgctcgcc ggcgatcttg attgccgaaa actgcggcgg ggtctgcatg
     1741 atgacgccgg tgtaattctg caatattgcc gccacatccg cctgagacgg gcgggtatcg
     1801 gaggttttcg tcaccggacc ttcgaggtcg tcggtggaac gctcctgccc ccaggcgacc
     1861 gtgaaacggt aaatcttggc gccatcctga acatagggca ccgtcttggt ggcgtcgccc
     1921 agcgcaatcg gcaacatgcc cgacgccagc gggtcgagcg tgccggcatg gccggccttt
     1981 tcagcctgga acagccattt gaccttggag acggcctcgg tcgaacccat gccgaccggc
     2041 ttgtccagca ccacccagcc ggagatcggc cggcccttct tcttgccgcg acgcgccact
     2101 attccttgtc ctcgccgtct tcgtcctgcc ccaggtcgcg cgccacttcc ggcgatttca
     2161 aaagatcgtt gatcttggcg aaattgtcga a
//
LOCUS       DASYCS010000148         1749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_31210, whole
            genome shotgun sequence.
ACCESSION   DASYCS010000148 DASYCS010000000
VERSION     DASYCS010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31210"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            261..>1749
                     /locus_tag="VGM46_04865"
     CDS             261..>1749
                     /locus_tag="VGM46_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009338484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY4191955.1"
                     /translation="MEIRNIAIIAHVDHGKTTLVDQLLKQSGSVRENQHMDERAMDSN
                     DQERERGITILAKCTSVEWNGVRINTVDTPGHADFGGEVERILSMVDGVVLLVDAAES
                     VMPQTKFVLAKALKLGLKPIVVINKIDRSDAQPAETLESIFDLFLALEANDSQLDFHH
                     LYASGRNGWAVNELEDERKDLSPLFKRIVEDVPPPKPQALASEEHPFKMLVTTLEADP
                     FLGRILTGRIESGVLTMGRNIKALNAAGEIIEKTRVTKLLAFRGLARVPVERAEAGDI
                     VAIAGLETATVADTLCDLTVAEAIPAHPIDPPTLSMTLSVNDSPYAGREGDKVQSRVI
                     RERLIREAEGNVALKVAETGDGAFEVSGRGELQLGVLIESMRREGFELSISRPRVVMK
                     TENGEKLEPIEEVTVDVDDAYTGTVIEKISQRKGEMVDMRPTGAGKTRIVFHGPARGL
                     IGYHGEFLTDTRGTGVMNRMFLEYAPHKGSVGGRINGVLISTEQGE"
BASE COUNT          360 a          583 c          526 g          280 t
ORIGIN      
        1 gccggtccct ggccggcctt ccggtctgga agattggcca tatcccgctt ctgcgacgtt
       61 attatagccc cggtatacgg caaatctctc tcacaaatgt ctcaggaaaa ctgcccaaaa
      121 acggctaaat tcggcggaaa agtcgcaaaa agtgtcgatt ttcaccgcca accggtaaag
      181 ggaacctgca aggcatgttg ccccgcagca ggggcgcctc tataagcccg gctttccgcg
      241 cgcaaaggcc actgattctc atggaaatcc gcaatatcgc gattatcgcg cacgtcgacc
      301 acggcaagac cacgcttgtt gaccagctgc tgaagcagtc gggctctgtg cgcgagaacc
      361 agcacatgga cgagcgcgcc atggactcca acgaccagga gcgcgagcgc ggcatcacca
      421 tcctggccaa gtgcaccagc gtcgaatgga acggcgtacg catcaacacc gtggacaccc
      481 ccggccatgc cgacttcggc ggcgaggtcg agcgcatcct gtcgatggtg gacggcgtgg
      541 ttctgctggt ggacgcggcc gaaagcgtca tgccccagac caagttcgtg ctcgccaagg
      601 cgctgaagct gggtctcaag cccatcgtgg tcatcaacaa gatcgaccgt tccgacgccc
      661 agcccgccga gaccctggaa tccatcttcg acctgttcct ggcgctggaa gccaatgaca
      721 gccagctcga cttccaccac ctctacgcct cgggccgcaa tggctgggcc gtcaacgagc
      781 tggaagacga gcgcaaggac ctgtcgccgc tgttcaagcg catcgtcgaa gatgtgcccc
      841 cgcccaagcc gcaagcgctg gccagcgaag aacatccctt caagatgctg gtgacgacgc
      901 tggaagccga tccgttcctg ggccgcatcc tgacgggccg cattgaatcg ggcgtcctga
      961 ccatgggccg caacatcaag gcgctgaatg ccgccggcga aatcatcgaa aaaacccgcg
     1021 tcaccaagct gctggccttc cggggcctgg cccgcgtgcc ggtcgagcgc gccgaagcgg
     1081 gtgacatcgt cgccattgcc ggcctggaaa ccgccaccgt cgccgatacg ctgtgcgacc
     1141 tgaccgttgc cgaagcgatc ccggcgcatc ccatcgatcc cccgaccctg tccatgaccc
     1201 tgtcggtcaa cgattccccc tatgccggcc gcgagggcga caaggtgcag agccgcgtca
     1261 tccgcgagcg gctgatccgc gaagccgaag gcaatgtcgc gctcaaggtc gccgaaaccg
     1321 gcgacggcgc tttcgaagtg tcgggccgcg gcgaactgca gctcggcgtg ctgatcgaat
     1381 ccatgcgccg cgaaggcttc gagctttcga tctcgcgccc gcgcgtggtg atgaagaccg
     1441 agaatggcga aaagctggag ccgatcgagg aagtcaccgt cgatgtcgac gatgcctata
     1501 ccggcaccgt catcgagaag atttcccagc gcaagggcga gatggtcgac atgcgcccga
     1561 ccggcgccgg caagacccgc atcgtgttcc acggcccggc gcgcgggttg atcggctatc
     1621 acggcgaatt cctcaccgac acgcgcggca ccggcgtgat gaaccgcatg ttcctggaat
     1681 atgccccgca caagggcagc gtcggcggcc gcatcaacgg cgtgctgatc tccaccgagc
     1741 agggcgaag
//
LOCUS       DASYCS010000149         3909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2062719,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000149 DASYCS010000000
VERSION     DASYCS010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2062719"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..1057)
                     /locus_tag="VGM46_04870"
     CDS             complement(53..1057)
                     /locus_tag="VGM46_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY4191956.1"
                     /translation="MSVMVKESPIDEKEMVAQAEKALADISLIREGVGRVIFGQESVI
                     ERTLVALLAGGHALLVGVPGLAKTKLVETLGTVLGLDASRIQFTPDLMPSDILGSEVM
                     EQDETGRRSFRFIQGPIFAQLLMADEINRASPRTQSALLQAMQEYHVTIAGARYDLPA
                     PFHVLATQNPLEQEGTYPLPEAQLDRFLMQIDILYPELEAERRILLETTGIEDAKAQN
                     VLAAERLKEIQTLIRRMPVSESVVEAILKLVRSARPGQGSAETDRFVAWGPGPRASQA
                     LTLCARARALYDGRLAPSVDDVRALAEPVLQHRMALTFAARAEGTTVRDVVAKLVKGI
                     "
     gene            1224..1853
                     /locus_tag="VGM46_04875"
     CDS             1224..1853
                     /locus_tag="VGM46_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HEY4191957.1"
                     /translation="MTDPISPDPQGLSSAKDARGLEALISRAARAGKGLPPVERWHPD
                     FCGDIDMEIRQDGTWFYMGTPIGRMPLVQLFSTVLRKDEDGKTYLVTPVERVGIRVVD
                     APFIAVEIDVSGSGDDQVITFRTNVGDVVEAGPEHPLRFVDEAETGGLKPYVLVRGRL
                     EALVSRAVMYELVGHGEEIEVDGALTFAVRSGGEIYPIMPAESLKRLSA"
     gene            1853..2485
                     /locus_tag="VGM46_04880"
     CDS             1853..2485
                     /locus_tag="VGM46_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEY4191958.1"
                     /translation="MDIQSASPFSAADFRARAAVQPQMRAEDDYGDHRFNPGHPRMSA
                     NRALRNAAVLIPVVDRGPQATVLLTKRAETLRNHSGQVAFPGGTIDATDPTPEFTALR
                     ETMEEIGLEESFIEVIGRMPDYVTGSGYRIAPVLGIVKPGFSLALNAHEVDAAFEVPL
                     SFLMDPANHRRDSRMWNDLEWFFYEMPYGGQRVWGVTAGIVRTVYERLYA"
     gene            2482..3765
                     /locus_tag="VGM46_04885"
     CDS             2482..3765
                     /locus_tag="VGM46_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018429053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEY4191959.1"
                     /translation="MKITIADKADWLRDRHLQSLLAALCEDGEEARIAGGAVRNALMG
                     QPVADVDIATTTLPRETIRRAEKTGFKAVPTGIDHGTVTVIAGGRPFEVTTLRADIET
                     DGRHAKVSFGRDWKADAERRDFTINALYAEADGTVIDLVGGLADMEARLLRFIGDPDQ
                     RIREDYLRILRFFRFFAWYGKGRPDAEGVKACARLKEGLDGLSAERVWAELKKLLCAP
                     DPSRALLWMRQSGVLTRVLPESEKWGIDAIHALVKAEGDLGWTPDPLARLEAIVPPDT
                     ARMKALAGRLKFSTREAERLKRWSLTGAVEPKTTEAELAKRLYRGDSQAIVDRLQLSL
                     ASARARAAGDGGAMDEKAMMETGGLSRLLAFAQKWEKPIFPLKGADLSALGAEPGPKL
                     GSVLKSLENDWIEANFTLDRGALLLHAADLLAKDS"
BASE COUNT          680 a         1196 c         1318 g          715 t
ORIGIN      
        1 gcgagcgcgt cgcgcgcggc agccggtgcc tggacctcgc cgatattggc catcaaatgc
       61 ctttcaccag cttcgccacg acatcgcgca cggtggtgcc ttcggcgcgg gcggcgaagg
      121 taagcgccat gcggtgttgc agcacaggct cggccagcgc gcgcacatcg tcgacggaag
      181 gggcgagccg cccgtcatag agcgcgcgcg cgcgggcgca cagcgtcagc gcctggctgg
      241 cgcgcgggcc cggaccccag gcgacgaacc tgtcggtttc agcgctgccc tggcctgggc
      301 gagccgagcg caccagcttg aggatcgcct cgaccacgct ttcggacacc ggcatgcgtc
      361 ggatgagggt ctggatttcc ttcaggcgtt ccgcagcaag cacgttttgc gccttcgcat
      421 cctcgatgcc ggtggtttcc agcaatatgc ggcgctcggc ttcgagttcg gggtaaagga
      481 tatcgatctg catcaggaag cggtcgagct gcgcctcggg cagcggatag gtgccctcct
      541 gttcaagtgg gttctgcgtc gccagcacat ggaacggggc cggcaggtcg tagcgcgcgc
      601 cggcgatggt gacatggtat tcctgcatgg cctgcaacag ggcggactgc gtgcgcggcg
      661 aggcgcggtt gatctcgtcc gccatcagaa gctgcgcgaa gatcggcccc tgaatgaagc
      721 ggaacgaacg gcgcccggtt tcgtcctgct ccatgacttc ggagccgaga atatcggacg
      781 gcatcaggtc gggcgtgaac tggatgcggc tggcgtcgag gccgagcacg gtgcccagcg
      841 tttcgaccag cttggtcttg gccagccccg gcacgccgac cagcagcgca tggccgccgg
      901 ctaaaagcgc caccagcgtg cgctcgatga cgctttcctg gccgaagatg acccggccga
      961 caccttctcg gataagcgaa atatcggcca gcgccttttc ggcctgggcg accatttcct
     1021 tttcgtcgat cgggctttcc ttgaccatca cgctcatgca gcatcgatcc ttgtggttgg
     1081 aaacggcgag ccgcacggtt tacttcccga tcttacgcca gattcggacc gtgccgcgat
     1141 tcgcgggctc gcgttgcgcc tcgaacgaac ttaaatcaca tagttgggct gacaagcgca
     1201 acaacagtga ctatttcgtg acgatgaccg atccgatttc tcccgacccg cagggcctta
     1261 gcagcgccaa agacgcgcgg gggctggaag cgttgatttc gcgcgccgcg cgagccggca
     1321 agggcctgcc gccggtcgag cgctggcatc ccgatttctg cggcgatatc gacatggaaa
     1381 tcaggcagga cggcacctgg ttctacatgg gcacgcccat cggccggatg ccgctggtgc
     1441 agcttttttc cacggtgctg cgcaaggacg aggatggaaa gacctatctg gtgacgccgg
     1501 tggagcgggt cggtatccgc gtcgtggacg cgccgttcat cgccgtcgag atcgatgttt
     1561 ccggctccgg cgacgatcag gtcatcacct ttcgcaccaa tgtcggcgac gtggtcgagg
     1621 cggggccgga gcatccgctg cgcttcgtcg acgaggccga gaccggcggc ctgaagccct
     1681 atgtgctggt gcgcgggcgg ctggaggcgc tggtctcgcg ggcggtgatg tacgaactgg
     1741 tcgggcatgg cgaggaaatc gaggtcgacg gcgcgctgac gttcgcggtg cggtcgggcg
     1801 gggaaatcta tccgatcatg ccggcggaaa gcctgaagcg gttgagcgcc tgatggatat
     1861 ccaaagcgca tcgccgtttt cggcggccga ttttcgcgcg cgcgccgccg tccagccgca
     1921 gatgagggcg gaagacgatt atggcgacca ccgcttcaac cccggccatc cgcgcatgag
     1981 cgcgaaccgg gcgctgcgca atgccgccgt tcttatcccg gtggtcgatc gtgggccgca
     2041 ggccaccgtg cttttgacca agcgggcgga aacgctgcgt aatcattcgg gacaggtggc
     2101 ctttcccggc ggcacaatcg acgccaccga tccgacaccg gaattcacgg cgctgcgcga
     2161 gacgatggaa gagatcggcc tggaggagag cttcatcgag gtgatcgggc gcatgcccga
     2221 ttatgtcacc ggcagcggct atcgcatcgc gcccgtgctt ggcatcgtca agccgggatt
     2281 ttcgctggcg ctcaacgccc acgaggtgga tgccgccttc gaggtgccgc tttccttcct
     2341 gatggaccct gccaaccatc gccgcgacag ccgcatgtgg aacgatctcg aatggttctt
     2401 ctacgaaatg ccctatggcg gccaacgcgt ctggggcgtt accgccggca tcgtccgcac
     2461 ggtctatgaa aggctatacg cttgaagatc accattgccg acaaggccga ctggctgcgc
     2521 gaccggcact tgcaaagcct gcttgccgcg ctttgcgagg atggcgagga agcgcgcatc
     2581 gccggtggcg cggtgcgcaa cgcgctgatg ggccaaccgg tcgccgatgt cgatatcgcc
     2641 acaacgacgc tgccgcgcga aaccatacgc cgggccgaaa agaccgggtt caaggcggtg
     2701 ccgaccggca tcgaccatgg caccgtcacc gtcattgccg gcggacgccc gttcgaggtg
     2761 acgacgctgc gcgccgatat cgaaaccgat gggcgacatg cgaaagtttc gttcgggcgt
     2821 gactggaaag cggacgcaga gcggcgcgac ttcaccatca acgcgctcta tgccgaggcc
     2881 gatggcacgg tgatcgatct ggtcggcggg ctggccgaca tggaggcgcg gctgctgcgc
     2941 ttcatcggcg atccggacca gcgcatccgc gaggattacc tgcggatatt gcggtttttc
     3001 cgctttttcg cctggtatgg caaaggacgg cccgacgccg agggcgtgaa ggcctgcgcc
     3061 cgcctcaagg agggccttga cgggctttcg gcggaacgtg tgtgggctga acttaagaag
     3121 ctgctttgcg cgcccgatcc gtcgcgcgcg ctgttatgga tgcgccagtc cggcgttttg
     3181 acgcgggttt tgccggaaag cgaaaagtgg ggcatcgacg ccatccacgc attggtcaaa
     3241 gcggaaggcg atctgggctg gacgcccgat ccgctggcgc ggctggaagc catcgttccg
     3301 cctgacacgg cgcggatgaa ggcactggct gggcggctga aattctccac ccgcgaggcc
     3361 gagcggctga agcgatggag cctgacgggc gcggtcgagc cgaaaacgac cgaggcggaa
     3421 ctggcgaagc ggctttatcg cggcgacagc caggctatcg tcgaccgctt gcagctgtcg
     3481 ctggcttcgg cgcgcgcgcg cgccgccggc gatggcggtg ccatggatga gaaggccatg
     3541 atggagacgg gtggtttgtc gcgcctgctt gctttcgcgc aaaaatggga aaagccgatt
     3601 tttccgctca aaggcgctga tctttcggcg ctgggcgctg agccgggacc gaaactcggt
     3661 tcggttctca agtcgctgga aaacgactgg atcgaggcga attttacgct cgatcggggc
     3721 gcgctgcttt tgcacgccgc cgacttattg gcaaaagaca gctagagcgg ttctggtttt
     3781 cactggatcg gcaaaaaccg ctccatctct ccgttttggc cgcattcccg gacgcaaatc
     3841 cgtttcacac ttttgccgga aatggtctag tcaggccgcg tcgcggacct tttcgatacg
     3901 cgagcggat
//
LOCUS       DASYCS010000150         3214 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4814775,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000150 DASYCS010000000
VERSION     DASYCS010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4814775"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VGM46_04890"
     CDS             complement(<1..390)
                     /locus_tag="VGM46_04890"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEY4191960.1"
                     /translation="MTRTIVASATREIIIGFDQPFCVIGERINPTGRKKLAAEMQAGN
                     FETVIKDALEQAACGATMLDINAGVTSVNPNETEPGLLVQTLEIVQGLVDLPLAIDSS
                     VSAAIEAALKVAKGRPLVNSVTGEEEKL"
     gene            complement(467..1558)
                     /locus_tag="VGM46_04895"
     CDS             complement(467..1558)
                     /locus_tag="VGM46_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEY4191961.1"
                     /translation="MIGRQRDENPAGVHLPLDPLPGHTSRGRLERVLRRGEFAVTTEL
                     NPPDSADPEEVYNRAKVFDGWVDAINAVDASGANCHMSSVGICALLTRMGYAPIMQIA
                     CRDKNRIAIQGDVLGGAAMGVANMLCLTGDGVQAGDQPGAKPVFDLDCMSLLETCRIM
                     RDNGKFLSGRKLTTPPQLFLGAAINPFAPPYDFRPHRLGKKIAAGAQFVQSQYCFDVP
                     MFRSYMQKVRDLGHTEKVFILCGVGPLASAKTAKWIRANVPGIHIPDAIIKRLEGAQD
                     QKKEGKQLCIDIINEVKEIPGVSGVHVMAYRQEEYVAEIVDESGVLKSRQPWKREARG
                     DDRMVAERLDHILRDDITETQVDMVKTAH"
     gene            complement(1555..2121)
                     /locus_tag="VGM46_04900"
     CDS             complement(1555..2121)
                     /locus_tag="VGM46_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HEY4191962.1"
                     /translation="MVKKAAPKSDYKPADVSPQRRVQRSFAVRLWSVRHSRLLEWFYA
                     RFADVFLLLHPLWKGIGYGRVEAPIKFIEKHVKGFMFDCRMCGQCVLSSTGMSCPMNC
                     PKQLRNGPCGGVRANGHCEVEPNMPCVWVKAWEGSQQMVHGDKILNVQKPVDQSLRET
                     SAWLRVTAQAAATREAAKAAASNSGATA"
     gene            complement(2213..2515)
                     /locus_tag="VGM46_04905"
     CDS             complement(2213..2515)
                     /locus_tag="VGM46_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HEY4191963.1"
                     /translation="MADLIVVYWRDIPAQVIVKRGRQNAKRELSLRFTEAIDMAAMRS
                     GAEGSSDYLSEWRKADPLPVSDDLEAEADKAAADLEAQFDRERLVALAKAGGKENV"
     gene            complement(2690..>3214)
                     /locus_tag="VGM46_04910"
     CDS             complement(2690..>3214)
                     /locus_tag="VGM46_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4893 domain-containing protein"
                     /protein_id="HEY4191964.1"
                     /translation="ALADGIVQKLITPADKSRLDQYSDTRKTALDEAKAGNAADLKQL
                     DALLARPLVSFSDRDLTGNWQCRTIKLGGDLGALVIYGWFKCKVTDDGSGWVLEKTSG
                     SQRTSGRFYDDGEKRSIYLGSYYVSGDPVKRYASGPGTDQVGYAFRTDANEFRIEFPA
                     PYYESRLDILELRR"
BASE COUNT          572 a          971 c         1020 g          651 t
ORIGIN      
        1 cagcttttct tcctcgccgg tgacggagtt gaccagcggg cgtcccttgg cgactttcag
       61 cgccgcctcg atcgccgccg aaacggaact gtcgatcgcc agcggcaggt cgacgaggcc
      121 ctgcacgatc tccagcgtct gaaccagaag gccaggctcc gtctcgttcg gattgaccga
      181 agtgacgccg gcgttgatgt cgagcatggt ggcgccgcag gcggcctgtt ccagcgcgtc
      241 cttgatgacg gtttcgaaat tgcccgcctg catttcggcg gcaagcttct tgcggccggt
      301 gggattgatg cgttcgccga tcacgcagaa aggctgatcg aagccgataa tgatctctct
      361 ggtcgccgag gcgacgatgg tgcgggtcat gtaatctctc cggtcatgat ataaagattt
      421 ctttatatcg ctatctgttt tgaatcaagt gaacgtcgcc ggcgcatcaa tgcgcggtct
      481 tgaccatatc cacctgcgtt tccgttatat cgtcgcgcaa gatgtgatcc agccgctcgg
      541 caaccatccg gtcgtcgccc cgcgcctcgc gcttccaagg ctgacggctt ttgagcacgc
      601 ccgattcatc gacgatttcg gcgacatatt cctcctggcg ataggccatc acatggacgc
      661 cggaaacgcc gggaatttcc ttcacctcgt tgatgatgtc gatgcagagc tgcttgcctt
      721 ccttcttctg gtcctgcgca ccttccagcc gcttgatgat tgcgtcgggg atgtggatgc
      781 ccggcacgtt ggcgcgaatc catttagccg tcttggccga ggccagcggg ccgacaccac
      841 acaggatgaa caccttctcg gtatggccga ggtcgcgcac cttctgcata tagctgcgga
      901 acatcggcac atcgaagcag tactggctct gcacgaattg cgcgccggcg gcgattttct
      961 tgccgagccg atgcgggcgg aaatcgtaag gcggggcaaa cgggttgatt gccgcgccca
     1021 ggaaaagctg gggcggcgtg gtcagcttgc gcccggacag gaatttgccg ttgtcgcgca
     1081 tgatgcggca ggtttccaga agcgacatgc agtcgaggtc gaacaccggc ttggcgccgg
     1141 gctggtcgcc ggcctgcacg ccgtcgccgg tcaggcacag catgttggcg acgcccatgg
     1201 cggcaccgcc cagcacgtcg ccctggatgg cgatgcggtt cttgtcgcgg caggcgatct
     1261 gcatgatcgg ggcgtagccc atgcgggtca gcagcgcgca gatgccgact gacgacatat
     1321 ggcagttcgc gccggaagca tcgacggcat tgatggcgtc gacccagcca tcgaaaacct
     1381 tggcgcggtt gtagacttcc tccggatcgg cgctgtcggg cgggttaagt tcggtcgtca
     1441 cggcgaactc gccgcggcgc agcacacgct ccagccgtcc gcgcgaggtg tggccgggaa
     1501 ggggatcgag cggcaggtgg acgcctgccg gattttcgtc gcgctgacgc ccgatcatgc
     1561 tgtggctccg ctattggagg ctgcggcctt tgcggcttcg cgcgtggcgg ccgcctgagc
     1621 cgtgacgcgc agccaggcgg acgtttcgcg cagcgactga tcgaccggct tttgcacgtt
     1681 caggattttg tcgccgtgaa ccatttgctg cgagccttcc catgccttga cccaaacgca
     1741 aggcatgttg ggttcgacct cgcaatggcc attggcgcgg acgccaccgc aagggccgtt
     1801 gcgcaactgc ttcgggcagt tcatcgggca ggacatgccg gtggaggaaa gcacgcactg
     1861 gccgcacatg cggcagtcga acatgaagcc tttcacatgc ttttcgatga acttgatcgg
     1921 cgcttcgacg cggccatagc cgatgccctt ccacaaagga tgcagtagca ggaaaacatc
     1981 ggcgaacctg gcgtagaacc actcaagcag gcgcgaatgg cgcaccgacc acagccgcac
     2041 cgcaaacgag cgctgtacgc ggcgctgcgg agaaacgtcg gcaggcttgt agtcgctctt
     2101 cggcgcggcc ttcttgacca tggccgcctg cgtcatgccg gcaggtgccg ccggcgcgac
     2161 ggtgcttgac gcaccggccg gtccggatgc cgttgtcgaa gccagctctt gttcagacat
     2221 tttccttgcc gcctgccttg gcgagagcga ccagccgctc gcggtcgaat tgcgcttcga
     2281 gatcggccgc cgccttgtct gcttcggctt cgaggtcgtc gcttacgggc agcggatcgg
     2341 ccttgcgcca ttcggacaga tagtcgctgg agccttcggc gccgctgcgc atcgcggcca
     2401 tgtcgatagc ctcggtaaag cgcagcgaaa gctcgcgctt ggcgttttgc cggccacgct
     2461 tgacgatgac ctgggcggga atgtcgcgcc agtagacgac gatcagatcg gccatgaaat
     2521 ttctcctcgt attttcgagc attgccgcaa gcgttgccgg gctgcttgtt atgggacgac
     2581 gcgcgcgcct tcaaaagcga cgaatggaga tgtcgcaaaa agaaagcctg ctgcggcggg
     2641 tcttgtagcg cacgccgccg gtggacaaat caggtttatg agctggcgtt caacgcctga
     2701 gttcgagaat gtcgagcctg gattcgtagt agggcgccgg aaactcgatg cgaaactcat
     2761 tggcatcggt gcggaaggcg tagccgacct gatcggtgcc ggggccgctg gcatagcgtt
     2821 tcaccggatc gccggaaacg tagtacgaac caagataaat cgagcgtttc tcgccgtcgt
     2881 cgtagaagcg gccgctggtg cgttgtgagc cgctggtctt ttccaaaacc cagccggagc
     2941 cgtcgtcggt taccttgcat ttgaaccagc cgtaaatcac cagtgcgccg agatcgccgc
     3001 caagcttgat cgtgcggcat tgccaattgc cggtcaggtc cctgtcggaa aaggaaacga
     3061 gcggccgcgc cagcaacgca tcaagctgct tcaggtcagc cgcgttgcct gctttggcct
     3121 catcaagggc cgtcttgcgc gtgtcggaat actggtcgag cctcgacttg tcggcaggcg
     3181 tgatgagctt ctgcacaata ccatcagcaa gcgc
//
LOCUS       DASYCS010000151         3347 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3367647,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000151 DASYCS010000000
VERSION     DASYCS010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3367647"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /locus_tag="VGM46_04915"
     CDS             <1..867
                     /locus_tag="VGM46_04915"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEY4191965.1"
                     /translation="FIYIGGNDTSGTQQILTDAAGGKMAFVHAPKTIDNDLEENDHVP
                     GFISAAEFVAGAFLSVDLDFRALPGIYVGIVMGRHAGFLTAASAAWRLDGDSGPHLVY
                     VPERAFSTKQFIDDVRATLDKHKRCIVAVSEGVSTADGKALVESLVPPERLERDQHGN
                     VKLSGSDLTAALEHALADGLPGKRARVDALGYMPRGYVGAINAVDAQEAFDAGAFAVS
                     VAEQGGGSVALQYEGGRTVLKKVPLKNVAGKTRHMPDDFMQADANRLSEAGIAYLKRL
                     VPEKHKVGKPFV"
     gene            880..1599
                     /locus_tag="VGM46_04920"
     CDS             880..1599
                     /locus_tag="VGM46_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY4191966.1"
                     /translation="MSGWFSKRIVDDRTTMLTEPFVHDYMRANIWHVRGRDMDLLVDT
                     GMGICPLAPEIDAPAGKPLLVVATHIHLDHVGSLHEFPLRAGPAQSAAEFGRMGDEAT
                     YAHEFRVLAGAVSQLPRPGWKAADYRIEPAPLTRILGEGDSVDLGDRQFKVLHLPGHS
                     PDSIGLFDEKDGTFFSGDAIYDDTLLDDMPDSDRAAYRLTMQRLLSLPIGVGHGGHGP
                     SFSRRRMIEIANEYLNRSKNL"
     gene            1998..2195
                     /locus_tag="VGM46_04925"
     CDS             1998..2195
                     /locus_tag="VGM46_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006331883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191967.1"
                     /translation="MKTIALTTAALLVAAGSAFAASDHFQGPSTQSSIDSAYTASIGD
                     NDKSVQKPVAEGNVRLTVGNH"
     gene            2256..2450
                     /locus_tag="VGM46_04930"
     CDS             2256..2450
                     /locus_tag="VGM46_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006331883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191968.1"
                     /translation="MKTIALATAALLVAAGSAFAGSDHFQGPTQSSIDNAHTASISSN
                     GAAVRKPVAEGNDRLTVGNH"
     gene            2505..2714
                     /locus_tag="VGM46_04935"
     CDS             2505..2714
                     /locus_tag="VGM46_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006331883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF680 domain-containing protein"
                     /protein_id="HEY4191969.1"
                     /translation="MKTIALTAAALLVAAGSAFAASDHFKAPSAQSNQSSTIDNSYTA
                     SVRTSDATVQKPVTEGNTNITAGSH"
     gene            complement(2815..>3347)
                     /locus_tag="VGM46_04940"
     CDS             complement(2815..>3347)
                     /locus_tag="VGM46_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY4191970.1"
                     /translation="FNRYPFDLVRDMEYDEPGGPITHGRFHTMTGYNLINQMTPSDVD
                     LFATLTMLASRFSIDYGILHTCTLDSMGHRFGHDCREMDHAVFAMDAMLAAFLPRWRE
                     AGYEVIVTADHGQTDRGHHGGHEDLQQDFALYYFGPAKGPKEDVLLDQLQLAPTVLKR
                     LGVPIPATMKAKPFLT"
BASE COUNT          693 a         1061 c          943 g          650 t
ORIGIN      
        1 ttcatctata ttggcggcaa cgacacgtcc ggcacccagc aaatcctcac cgatgcggca
       61 ggcggcaaga tggccttcgt ccacgcgccg aaaaccatcg ataacgacct tgaagaaaac
      121 gaccacgttc cgggcttcat ctcggctgcc gaattcgtcg ccggcgcttt cctgtcggtc
      181 gatctcgatt ttcgcgcgct gcccggcatc tatgtcggca tcgtcatggg ccgccacgcc
      241 ggcttcctga cggcggcatc cgccgcctgg cggctggatg gcgacagcgg cccgcatctg
      301 gtctacgtgc ctgagcgcgc tttttcgacc aagcagttca tcgatgatgt gcgcgccacg
      361 ctcgataagc acaagcgttg catcgtcgcc gtatcggaag gcgtttccac cgccgacggt
      421 aaggcgctgg tcgaaagcct ggtgccaccg gaaaggctgg agcgcgacca gcatggcaac
      481 gtcaagctgt cggggagcga cctgacggcg gcgctggaac atgcgctggc cgatggcctg
      541 ccgggcaagc gcgcccgcgt cgatgcactg ggctacatgc cgcgcggtta tgtcggcgcc
      601 atcaatgccg tcgatgcgca ggaagccttc gatgccggcg ccttcgccgt cagtgtcgcc
      661 gaacagggcg gcggctccgt cgcgctgcaa tatgagggcg gcaggacggt gctgaagaaa
      721 gtgccgctga aaaacgtcgc cggcaagacg cgccatatgc ccgacgattt catgcaggcc
      781 gacgccaacc ggctttccga ggccggcatc gcctatctca agcggttggt tccggagaaa
      841 cacaaggtag gcaaaccctt cgtttaaagg cgcgccatga tgtccggctg gttctcgaag
      901 cgcatcgtcg acgacaggac gacgatgctg acggaaccgt tcgtccacga ctacatgcgc
      961 gccaatatct ggcatgtgcg cgggcgcgac atggacctgc tggtcgatac cggcatgggc
     1021 atctgcccgc tggccccgga aattgacgcg ccggcgggca agccgctgct ggtcgtcgcc
     1081 acccacatcc atctcgatca tgtcggctcg ctgcacgaat ttccgctgcg tgccggcccg
     1141 gcgcaaagtg ctgccgaatt tggccgcatg ggcgacgagg cgacctatgc gcacgagttc
     1201 cgcgtgcttg ccggcgcggt ctcgcaattg ccccggcccg gctggaaggc cgccgactat
     1261 cgcatcgagc cggcgccgct gacccgcatt cttggtgaag gcgatagcgt cgatcttggc
     1321 gaccgccagt tcaaggtgct gcacctgccc ggccattcgc cggattcgat cggcctgttc
     1381 gatgaaaagg acggcacctt tttcagcggt gacgccatct atgacgacac gctgctggac
     1441 gacatgccgg attccgaccg cgccgcctat cgccttacca tgcaacgcct gcttagcctg
     1501 cccatcggtg tcggccatgg cggacacggc ccgagttttt cgcgccggcg catgatcgaa
     1561 atcgccaacg aatatctgaa tcgctccaag aatctttgac tgatcggcca aagcagcgcc
     1621 agataacccg tcattttagc gcctggcgtg cttcgttgag ggcggtgttt cgaccgttca
     1681 actgtctagc cggctaaaac taaaccgatt tacgtcgcca tatgttgacg ctgtgtgcag
     1741 gacggtgtga acgctggcct gaaaattcat tagccggaaa attcaaaaat attataacta
     1801 tttgattaca atagggaaaa ttgtggcgaa agagagcttt ggcacaattt caaccaccag
     1861 actcttggtc caggcagaaa aatttcgtga ctggaaagtt cgacaccgct cccccacatc
     1921 ggggtggtcc gaacgacgcg cttcattttt acggcacgcc atttgaggga cgagattaaa
     1981 aactggagta tttaaaaatg aagaccattg ctcttaccac cgccgctctc cttgtcgccg
     2041 ctggcagcgc tttcgccgca agcgaccact tccagggccc gtcgacccag tcgtccatcg
     2101 acagcgctta caccgcttcc atcggtgaca acgacaaatc cgttcagaag ccggttgccg
     2161 aaggcaatgt gcggctcacg gtcggcaatc actaacaaca acgatcagcg cccgccaaga
     2221 gacgggcgca tcctgaagaa tctggagtat ctgaaatgaa gaccatcgca cttgccactg
     2281 ccgccctcct tgttgccgcc ggtagcgcct tcgccggcag cgaccacttc cagggcccga
     2341 cgcagtcgtc catcgacaac gcccacaccg cttccatttc cagcaacggc gctgccgttc
     2401 gcaagccggt tgcagagggc aatgaccgcc tcacggtcgg caatcactaa caaccatcaa
     2461 cgtcccgctt gacggacgat atttgaaaat ctggagtact cgaaatgaag accattgctc
     2521 ttaccgccgc cgctctcctc gttgccgccg gcagcgcctt tgccgcgagc gaccacttca
     2581 aggccccttc ggcccagtcg aatcagtcgt cgacgatcga caattcttac acggcctccg
     2641 tgcgcaccag cgacgcgacc gtgcagaagc cggtaaccga aggcaacacg aatattaccg
     2701 ccggcagcca ctaggcttag cggttattca atcaaaacgg cgggcttgcc cgccgttttg
     2761 cttttttcgc caaagcctaa acgcttcgac gctatcgcct tttccgagcc ggtttcacgt
     2821 caggaatggc ttggccttca tcgtagccgg tatcggcacg ccaaggcgct tcagcaccgt
     2881 cggcgccaac tgcaactggt cgagcagcac atcctctttc ggcccttttg ccggtccgaa
     2941 ataatacagc gcgaaatcct gctgcaaatc ctcatggccg ccatgatgac cgcgatcggt
     3001 ctggccgtga tcggcggtta cgatcacctc gtagcccgct tcccgccagc gcggcagaaa
     3061 agccgccagc atggcgtcca tcgcaaacac cgcatgatcc atttcacggc aatcatggcc
     3121 gaaccgatgg cccatcgaat ccagggtgca ggtgtgcagg atgccgtaat cgatggaaaa
     3181 gcggctggcc agcatcgtca aagtggcgaa caggtcgaca tcgctcggcg tcatctggtt
     3241 gatgagatta tagccggtca tcgtgtggaa acggccatgc gtgatcggac cgcccggctc
     3301 gtcatattcc atgtcacgga ccagatcgaa cggatagcgg ttgaaga
//
LOCUS       DASYCS010000152         4466 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_904556,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000152 DASYCS010000000
VERSION     DASYCS010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4466
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_904556"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VGM46_04945"
     CDS             <1..219
                     /locus_tag="VGM46_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEY4191971.1"
                     /translation="FGAENGFFWAAVGGLIRIFLSDYFRLKKSHGLSHYTEAEMLTHL
                     KAAGYSAKRAERNIGHNPWRMTFLAKPE"
     gene            complement(356..1195)
                     /locus_tag="VGM46_04950"
     CDS             complement(356..1195)
                     /locus_tag="VGM46_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY4191972.1"
                     /translation="MTNPSKLPFSTESGEIPIIGFGTSPLTGGMTADAVLNALKTGYR
                     HIDTGRKYGTEDAVGEAIRKSGVPRQDIFLVTKVSHENLRPRDFAKSVDDSLAALKVD
                     YLDLLMIHWPNPAIPPSECIPALAKAKKDGKARHIGVANFNTALLDEAMAISSEPITV
                     LQAEYHPYLDQTKLLAAARRHKLVFVAYCPLARGRMFEDKVLGDIAKAHGRGIAQIAL
                     RWLIQQDVVAIPFSQNPQRIANNYDVFDFELSDEEMKRIAALKRPDGRIATPVERVAG
                     GWD"
     gene            1331..1588
                     /locus_tag="VGM46_04955"
     CDS             1331..1588
                     /locus_tag="VGM46_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4191973.1"
                     /translation="MTNREHIVRTAYARAWEDFERIPQTLPERRLAPQRLRQHILTVL
                     DGGERDPEKIAEEALGRLREAEQIARSRARVMKADGLSDAG"
     gene            complement(1604..2563)
                     /locus_tag="VGM46_04960"
     CDS             complement(1604..2563)
                     /locus_tag="VGM46_04960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEY4191974.1"
                     /translation="MPGWLASLAVIGSYATIAALGNRRCPRPVPVRKPRRGWAAWKHV
                     LFRTYEEINNDRLLALAAGVVFYGLLALFPAVTALVSSYALFAKASTINAHLALMSGV
                     MPASAYQIVDEQVTRIVQRGIGDLGAAFFIGLALAVWSANAGVKAVIDALNICYGVKE
                     RRGFFKLNAVSLLFTVGAIAMLLLAIAAVVVLPVVMSFLHAGRYGELAVTWLRWPAML
                     LIVLLGLATLYRFGPDVDNARWRWISPGAAFAAVAWLGGSALLSWYLASFADYDKTYG
                     SLGAAIGLMMWLWVTAIVVLVGAELNSEIDTEEAPVPKETAAF"
     gene            2892..>4466
                     /gene="carB"
                     /locus_tag="VGM46_04965"
     CDS             2892..>4466
                     /gene="carB"
                     /locus_tag="VGM46_04965"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF003671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEY4191975.1"
                     /translation="MPKRSDIKSILIIGAGPIVIGQACEFDYSGTQACKALKEEGYRV
                     ILVNSNPATIMTDPDLADATYIEPITPEIVTKIIEKERPDALLPTMGGQTALNCALSL
                     KKMGTLDKYNVKMIGATAEAIDKAEDRELFRNAMTKIGLETPRSHHVKSLGDALKALD
                     DIGLPAIIRPSFTMGGTGGGIAYNKAEFIEIVEGGIDASPTDEVLIEESVLGWKEYEM
                     EVVRDKKDNCIIICSIENIDPMGVHTGDSITVAPALTLTDKEYQMMRDASLAVLREIG
                     VETGGSNVQFAVNPEDGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     IANDITGGATPASFEPTIDYVVTKIPRFAFEKFPGASNVLTTSMKSVGEAMAIGRTFQ
                     ESLQKALRSLETGLTGLDEIAIEGFDPAKDIEDADNKAAIRAALGTPTPDRLLKVAQA
                     MRLGASDELVYNACRIDRWFLSEIRALIEMEGEIKAKGLPDTAGPFRRLKAMGFSDTR
                     LGKLTGTPADTVMQKRR"
BASE COUNT          920 a         1410 c         1348 g          788 t
ORIGIN      
        1 ttcggcgccg agaatggatt tttctgggcc gcggtcggcg gcctgatccg gattttcctg
       61 tcggattatt tccggctgaa gaaatcgcac ggcctgtcgc attacaccga ggccgagatg
      121 ctcacgcacc tcaaggctgc cggttattcg gccaagcgcg ccgaacgcaa catcggccac
      181 aatccgtggc gcatgacgtt cctcgccaaa ccggaataag ctttacttcc gctcttcccc
      241 gcgcatagcc gttcgcagga cggcgttctt tcagaacgcc tatgagcggg gacccagttc
      301 tttctttttc ttagctcctg gattcccgct tgcgcgggaa tgagcggaga ttgggtcagt
      361 cccagccgcc ggcgacacgc tcgaccggcg tggcgatacg gccgtcgggc cgcttgagcg
      421 cagcgatacg tttcatctct tcatcgctca attcgaaatc gaacacgtca taattattgg
      481 cgatgcgctg cggattctgc gaaaacggaa tggccacgac gtcttgctgg atcagccagc
      541 gcagcgcgat ctgcgcgatg ccgcggccat gcgccttggc gatgtcgcca agcaccttgt
      601 cctcgaacat gcgcccgcgc gccaacggac agtaggcgac gaagacgagc ttgtgccgcc
      661 gtgccgccgc caacagcttg gtctggtcga gatagggatg atactcagcc tgcaacacag
      721 tgatcggctc cgatgaaatc gccatcgcct cgtcaagtag cgcagtattg aaattagcga
      781 cgccaatgtg ccgcgccttg ccatcctttt tggccttggc aagcgccggt atgcattcgc
      841 tcggtggtat cgccgggtta ggccagtgaa tcatcagcag gtcgagataa tcgaccttga
      901 gcgccgcgag actgtcgtcg accgatttgg cgaaatccct gggccgcaaa ttttcgtggc
      961 tgaccttggt gactagaaaa atatcctgcc gtggcacgcc ggatttgcgg atcgcttctc
     1021 ccaccgcgtc ctcggtgccg tatttgcggc ccgtatcgat gtgacgatat ccggttttta
     1081 gcgcgttcag aacggcatcg gccgtcatgc cgccggtcaa cggtgaggta ccgaagccaa
     1141 tgatcggaat ttcgccgctt tcggtggaaa acggcagctt ggaggggttc gtcatgagtc
     1201 gcccgtaaaa ttgcggccaa gatttcgccg catcattatc cgaaacaggg ctggctgtat
     1261 caaagtttaa cgatggttgt ggtgcgccgc ctccggctat gccggagcgc gcgatgcggg
     1321 ggacctagac atgaccaacc gcgaacatat cgttcgaacg gcctacgccc gggcatggga
     1381 agatttcgaa cgcattccgc agacgctacc ggaacggcgt cttgcgccgc agcggctacg
     1441 ccagcacatc ctgacggttt tggatggagg cgagcgcgat cctgaaaaaa ttgccgagga
     1501 agcgctgggc cggctgcgcg aagccgagca gatcgcacgg tcacgcgccc gggtgatgaa
     1561 ggccgacggc ctttccgacg ccggctagtc tttttggcca acactagaat gcggccgtct
     1621 cctttggaac gggtgcttct tccgtgtcga tttccgaatt caactctgcg ccgaccagca
     1681 cgacgatcgc cgtcacccag agccacatca tcaggccgat ggcggcgccg agtgagccgt
     1741 aggttttgtc gtagtcggcg aaactcgcga gataccacga cagcaatgct gacccgccaa
     1801 gccaggcgac ggctgcgaac gccgcgcccg gactgatcca gcgccagcgc gcattgtcga
     1861 catcgggacc gaaccgatag agcgttgcga ggccgagcag cacgatcagc agcattgccg
     1921 gccaacgcag ccaggtgact gcaagttcgc cgtagcggcc agcatgcaag aacgacatga
     1981 caacgggcag gacgaccacg gccgcgatcg ccagcagcag catggcgatg gcgcccacag
     2041 tgaacagaag cgagacggcg ttgagcttga agaagccgcg gcgctccttg acgccgtaac
     2101 agatgttgag cgcgtcgatg accgctttca ccccggcatt ggcgctccac accgccagcg
     2161 ccaggccgat aaagaatgcc gcgccgaggt cgccgatgcc gcgctgcacg atacgggtca
     2221 cctgctcgtc gacgatctgg taggcgctag ccggcatcac gcccgacatc agcgcgaggt
     2281 gcgcattgat cgtcgaggcc ttggcaaaaa gcgcgtagct cgacaccagg gcggtgactg
     2341 ctggaaacaa ggctagcaaa ccgtaaaaaa cgacaccggc cgcgagcgcc aaaagccggt
     2401 cgttgttgat ctcctcataa gtccggaaaa ggacgtgctt ccaggccgcc cagccgcggc
     2461 gtggcttacg gaccgggacc ggacgcgggc agcgccggtt gccgagcgcc gcaatggtcg
     2521 catagctgcc aatgacggcc aggctggcca gccagcccgg cacccgacgt ccttgaaatg
     2581 ggcggcccgc caacgacata cagtcataac gtcggccggg tagacttagt ttcgacagac
     2641 ctgtggccag ggggcctgtt gcccttccca ccccctcgat tctggtctag agaccgctcg
     2701 cacggggtaa cccccgcttg ggggccgagg acgcagcgcg catttagcaa aagtgggcac
     2761 cggttttgct aaaatgcgca cagttttaaa ctagcgcgct ttccgggcgg ccaaccggtt
     2821 cccacttggc ctgaaagcgc gcgatgagcg cgcccttttt ttgtgtgcag tttggagccc
     2881 gacgttttcg aatgccgaaa cggtccgaca tcaaatccat cctgatcata ggcgccggcc
     2941 cgattgtcat cggccaggcc tgcgaattcg attattccgg cacccaggcc tgcaaggcgc
     3001 tgaaggaaga gggctaccgg gtcattctgg tgaactccaa tccggcgacg atcatgaccg
     3061 atccggacct ggccgacgca acctacatcg agccgattac cccggagatc gtcacaaaga
     3121 tcatcgagaa ggagcgtccc gacgctctgc tgccgaccat gggcggtcag accgcgctga
     3181 actgcgcgct gtcgctcaag aaaatgggca cgctcgacaa atacaacgtc aaaatgatcg
     3241 gcgcgaccgc cgaggccatc gacaaggcgg aagaccgcga gctgttccgc aacgccatga
     3301 ccaagatcgg tctggagacg ccgcgctcgc atcatgtgaa atcgctgggc gatgcgctca
     3361 aggcgctcga cgatatcggc ctgcccgcca tcatccgccc atcgtttacg atgggcggca
     3421 caggtggcgg catcgcctac aacaaggcgg agttcatcga gatcgtcgaa ggcggcatcg
     3481 acgcctcgcc gaccgatgaa gtgctgatcg aggagtccgt actcggctgg aaagagtacg
     3541 agatggaggt ggtgcgcgac aaaaaagaca attgcatcat catctgctcg atcgaaaata
     3601 tcgacccgat gggcgtgcat accggtgact ccatcaccgt cgcgccggcg ctgacgctga
     3661 ccgacaaaga ataccagatg atgcgcgatg cctcgctcgc cgtgctgcgc gagatcggcg
     3721 tcgagaccgg cggttcgaac gtgcagttcg ccgtcaaccc ggaagacggc cggctcatcg
     3781 tcattgaaat gaacccgcgc gtgtcgcgtt cgtcggcgct ggcatcgaaa gcgaccggct
     3841 ttccgatcgc caaggtcgcc gccaagctcg cggtcggcta cacgctcgac gaaatcgcca
     3901 acgacatcac cggcggcgcg acgccggcgt cgttcgagcc gaccatcgac tacgtcgtca
     3961 ccaaaatccc gcgtttcgcc ttcgagaaat tccccggcgc gtcgaacgtg ctgaccacgt
     4021 cgatgaagtc ggtcggcgaa gccatggcca tcggccgcac cttccaggaa agcctgcaga
     4081 aggcgctgcg ttcgctcgag accggcctca ccggcctcga tgaaatcgcc atcgaaggtt
     4141 tcgatcctgc caaggacatc gaggacgccg acaacaaggc cgcgatccgc gccgcgctcg
     4201 gcacaccgac gccggaccgg ctgctcaagg tcgcgcaagc catgcggctc ggcgccagcg
     4261 acgaactcgt ctacaacgcc tgccgcatcg accgctggtt cctgtcggaa atccgcgcgc
     4321 tgatcgagat ggaaggcgag atcaaggcca agggcctgcc ggacaccgcg ggcccgttcc
     4381 gccgtctcaa ggccatgggt ttctccgaca cccgtctcgg caaactcacc ggcacgccgg
     4441 ccgacaccgt gatgcagaag cgccgc
//
LOCUS       DASYCS010000153         4725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2932646,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000153 DASYCS010000000
VERSION     DASYCS010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4725
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2932646"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /gene="rplU"
                     /locus_tag="VGM46_04970"
     CDS             complement(<1..301)
                     /gene="rplU"
                     /locus_tag="VGM46_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018430100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEY4191976.1"
                     /translation="MDSAQSAWKLFRAACLLNPQTDKKTAEPPLFPRIPVHRGRPNSE
                     RQKMFAVIKTGGKQYRVAANDLLKVEKVAGNAGDIVEFTEILAVGEGENAEIGAPF"
     gene            complement(412..501)
                     /locus_tag="VGM46_04975"
     tRNA            complement(412..501)
                     /locus_tag="VGM46_04975"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(465..467),aa:Ser,seq:cga)
     gene            904..1035
                     /locus_tag="VGM46_04980"
                     /pseudo
     CDS             904..1035
                     /locus_tag="VGM46_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611606.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1058..2125)
                     /locus_tag="VGM46_04985"
     CDS             complement(1058..2125)
                     /locus_tag="VGM46_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HEY4191977.1"
                     /translation="MIAKLNVVPFVSVDSMMKLVLTIGVERFLTELAAYVEEDFRRWE
                     LFDKTPRIASHSHDGVIELMPTSDGHLYGFKYVNGHPKNMREGRQTVTAFGMLADVGN
                     GYPMLLTEMTILTALRTAATSAVAARYLAPKGARTMAVIGNGAQSEFQAIAFKALLGV
                     DRLRLYDIDASASQKCAKNLSGLGFDITICRSAQEVVEGAQIITTVTADKQCATILTD
                     NMVGGGVHINAVGGDCPGKTELHRDILLRSDIFVEYPPQTRIEGEIQQLEADHPVTEL
                     WQVMTGSAKGRSDTGQITLFDSVGFAIEDFSALRYVRDQLKATGLFEELDLLADPDEP
                     RDLFGMLLRAAKAGATTATAI"
     gene            complement(2122..3048)
                     /gene="rocF"
                     /locus_tag="VGM46_04990"
     CDS             complement(2122..3048)
                     /gene="rocF"
                     /locus_tag="VGM46_04990"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HEY4191978.1"
                     /translation="MHCTILGAPLQAGSGRMGCEMGPSALRTAGLAAALSDLGHTVTD
                     LGTVTPAKTAPISHPNPALKALPEIAAWTAALADAAYAASAGGMPIFLGGDHAISAGT
                     VSGLSRRAAETGRPLFVLWLDAHPDFHTLDSTASGNLHGVPLAYASGQPGFENYFPTL
                     HAAVPADRICVLGVRSVDPAERQALTTAGVTVHDMRAIDEHGIARLLRDFLERVAEAG
                     GMLHVSLDVDFLDPSIAPAVGTTVPGGATIREAHLVMEMLHDSGLVTSLDLVELNPFL
                     DERGRTATLMVDLTASLMGRRIMDRPTRSYGS"
     gene            3174..3614
                     /locus_tag="VGM46_04995"
     CDS             3174..3614
                     /locus_tag="VGM46_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010659538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY4191979.1"
                     /translation="MDSLDEKLVTLLRHDARRSISDLAIHLGVSRATVRSRMERLERA
                     GEIVGYTVILRADAVELPVRGIMMIEIEGHAADRAIRALGGFAEISTIHTTNGRWDLV
                     VELATATLTDFDAVLRRIRLIPGVTGSETNLLLATPRSTKARLG"
     gene            complement(4061..4360)
                     /locus_tag="VGM46_05000"
     CDS             complement(4061..4360)
                     /locus_tag="VGM46_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEY4191980.1"
                     /translation="MDHTKHIRLTTNELTPDILDDATIYGPGDEKVGSVSHVHGTGVA
                     TQVVIDVGGFLGIGAKPVSVPANQLDFMRDEDGDVHAVTSWTKDQLKDMPEHHEA"
BASE COUNT          833 a         1392 c         1602 g          898 t
ORIGIN      
        1 cgaagggtgc gccgatctcg gcattctcac cctcgccgac ggcgaggatc tcggtgaatt
       61 caacgatgtc accggcattg ccggcaacct tctcgacctt caaaaggtca ttggcggcca
      121 cgcgatactg cttgccgccc gttttaatga ctgcgaacat tttttgcctt tcgctgttcg
      181 gtcggcctcg atgaaccggt atcctcggga agaggggcgg ttcggccgtc tttttgtcag
      241 tctgcgggtt caatagacaa gcggcgcgga aaagcttcca cgccgattgc gcgctgtcca
      301 tagacgcagg cccggttgaa gtcaagacag gagcggcttt ggcgccaggc ggaaatgccc
      361 gggtggaaaa aggaagtcag cgcaggaaaa cgggtcaacg caggaagata ttggcggaga
      421 gggagggatt cgaacccccg atgggcttgc acccatgccg catttcgagt gcggtgcatt
      481 cgaccactct gccacctctc cgcgggtcat ggtccgccgt tgccggcgcg gcgcctagat
      541 agcggctcgg cgcttaggtt acaagggcca atctccacgg gtttgacggg agatgcggcg
      601 gcttgtcgag aggcgggccg gcggcagtag ggccgtgggg gaataggaca gtaaggcagt
      661 aaaggggcgg ggaagacgcg ggcaggccgg ggcgggcaag caggttgggg ctgccagcgg
      721 caaggccttg ccccgtgagt gcgttcccca gcgggcaaca ccggcttagc gtgggcaggt
      781 aggcaggtca ccagccgcgc ctgccggacg agttcagtgc cttccggacg gctggcgcga
      841 aagccttggc gagcatcccc agctgctcgg gccgcatcgt cagacgctca attgacatga
      901 gccatttacc gacagcgaaa ccaggtcgaa cacttcttcc gccgactcaa acacttccga
      961 tgcgtcgcca cccgcttcga caaactcgcc cgcaacttcc tcgcagccgt cctgctcgct
     1021 tcaatcaggc tgtgaccaga acttatgaac tcacaaccta gatcgccgtg gctgtcgtcg
     1081 cgcctgcctt ggcggcgcgc agcagcatgc cgaacaggtc gcgcggttcg tcggggtcgg
     1141 ccagcaggtc gagttcttcg aataggccgg tcgccttcag ctggtcgcgc acgtagcgga
     1201 gggcggaaaa gtcctcgatg gcgaagccga cggaatcaaa cagcgtgatc tggccggtat
     1261 cgctgcggcc tttggcgctt cccgtcatca cctgccatag ttcggtgacc ggatgatcgg
     1321 cttctagctg ctggatttcg ccctcaatgc gggtttgcgg cggatattcg acgaaaatgt
     1381 ccgagcgaag caggatgtcg cggtgcagtt cggtcttgcc gggacagtcg ccgccgaccg
     1441 cgttgatgtg gacgccgccg cccaccatgt tgtcggtcag gatggtcgcg cactgcttgt
     1501 cggcggtgac cgtggtgatg atctgggcgc cttccaccac ttcctgcgcc gagcggcaga
     1561 tggtgatgtc gaagcccagg ccggaaaggt ttttcgcgca tttttgcgag gccgatgcgt
     1621 cgatatcgta gaggcgcagc ctgtcgacgc cgagcagcgc cttgaaggcg atggcctgga
     1681 attcggactg cgcgccattg ccgatgacgg ccatggtcct tgcgcctttc ggggccagat
     1741 atctggcggc gacggccgag gtcgccgctg tgcgcagggc ggtgaggatg gtcatctcgg
     1801 tcagcagcat cggatagccg ttgccgacat cggccagcat gccgaaggcg gtgacggtct
     1861 ggcgtccctc gcgcatgttc ttcgggtggc cgttgacata tttgaagccg tagagatggc
     1921 cgtcgctggt tggcatcagt tcgatgacgc cgtcatggct gtgcgaggcg atgcgcggcg
     1981 tcttgtcgaa caattcccag cgccggaaat cctcctcgac ataggcggcg agttcggtga
     2041 ggaaacgctc caccccgatg gtgagcacca gcttcatcat gctgtcgacg ctgacgaagg
     2101 gaaccacatt cagtttggcg atcatgatcc atagctccgg gttgggcgat ccatgatgcg
     2161 gcggcccatc aggcttgccg tcaggtcgac catgagggtg gcggtgcggc cgcgctcgtc
     2221 gaggaagggg ttgagttcga ctaggtcgag gctggtgacg aggccgctgt cgtgcagcat
     2281 ctccatcacc agatgcgcct cgcggatggt cgcgccgccg ggaacggtgg tgccgacggc
     2341 gggtgcgata gaaggatcga ggaaatcgac gtcgaggctg acatgcagca tgccgccggc
     2401 ctcggccacg cgctccagaa aatcgcgcag aagcctggcg atgccgtgtt cgtcgatggc
     2461 gcgcatgtca tgcacggtga cgccggccgt ggtcagcgcc tgccgctcgg cgggatcgac
     2521 gctgcgcacg ccgagcacgc agatgcggtc cgcaggtacc gccgcatgaa gggtggggaa
     2581 atagttttcg aaccccggct gtccgctggc ataggccagc ggcacgccat gcagattgcc
     2641 gctggcggtg ctgtcgagcg tatggaaatc cgggtgggca tcgagccaca gcacgaacag
     2701 cggccgcccg gtctcggccg cacgccgcga caggccggaa acggttccgg cggaaatcgc
     2761 gtggtcgccg ccaaggaaaa tcggcatgcc cccggcgctg gcggcatagg cggcgtcggc
     2821 aagggccgcc gtccaggccg caatttcggg aagcgccttc agcgcagggt tcggatggct
     2881 gatcggtgcg gtcttggccg gcgtcaccgt gccgaggtcg gtcacggtat ggccaagatc
     2941 ggacagggcg gcggccaggc cggcggtgcg cagcgcgctc gggcccatct cgcagcccat
     3001 gcggcctgag cctgcctgga gcggcgcccc caatatcgtg caatgcatcc ggctctctcc
     3061 atgctgtctg tcgtgagtag agccgagcgg actggcgata tgaacgcgat gaattggcaa
     3121 atgagtcagg atcgcttatc aaaacggcag ctctggcaac caaaacggaa atgatggatt
     3181 ctctggatga aaaactggtg acgctcctgc gtcacgatgc gcggcgcagc atctccgatc
     3241 tcgccatcca tcttggcgtt tcgcgcgcaa cggtgcggtc gcggatggag cggctggaac
     3301 gggcgggcga gatcgtcggc tacacggtca tcctgcgcgc cgacgcggtc gaattgccgg
     3361 ttcgcggcat catgatgatc gagatcgagg ggcacgcggc agaccgggca atccgggcgc
     3421 tgggcggctt cgccgaaatc tcgacaatcc ataccaccaa cggacgctgg gacctggtgg
     3481 tggaactggc gacggcgacg ctgaccgatt tcgatgccgt gctgcgccgc atccggctca
     3541 taccgggcgt taccggcagc gagaccaacc tgcttctggc gacgccgcga agcaccaagg
     3601 cgcggctggg ctgagcgtag ccatgtgcat cggatgccgg cttttatgcg ccgggtggtg
     3661 attttcggcc ggaatgtcga tagcggtcag ttatccacgg ccagcgcctt ggtgttgtag
     3721 gcaactggcg agcatcgcgc agggctttgc atggctggcg tgtcgggcag caccgtttgc
     3781 cgcttcaagg atatagccgt tttcggctgt tgagtgctct cacccgtaat agcttcgacc
     3841 tgatggccgg cagcgcataa ccggcgctgg cagcggcctc gctttcggat attcctggtg
     3901 gggcgaggta cgcccacgcc atcacatgca ccgcgcacct gcacgtttct acctacgccg
     3961 aactgtcttc agccggtaaa tcgccggcag gctttcccgg agaagttttt aactcgtggc
     4021 gcggtgcaca tcattcccgg gcttaggcgg ggctatatct tcaggcctcg tgatgctcgg
     4081 gcatgtcctt gagctggtcc ttggtccatg acgtcacggc gtgaacgtcg ccgtcctcgt
     4141 cgcgcatgaa gtccagctgg ttggcgggga cggaaacggg cttggcgccg atgccgagga
     4201 agccgccgac atcgatcacc acctgcgtcg ccacgccggt gccgtggaca tgggagacgg
     4261 aaccgacttt ctcgtcgccg gggccgtaaa tagtggcatc gtcgaggatg tcaggcgtca
     4321 gttcgttggt ggtgagccgt atatgcttgg tgtggtccat tgcgcatctc cttggtggct
     4381 ggttcttgtt ggctggactg ggctaacccc cgacaaagcc atccgttccg cacaacgccg
     4441 ggtaaatgac aaatgtgatc gcgcggtttc cagcgcggcg cgacccatgg cctgctccct
     4501 tgagggcgga gctgatgctg gctgaagccg ggatgacata gtgagccgcc gcctcatgaa
     4561 ggcgtcgagt ttttgccgtc gataaaccgg gtttgccggg cgacgatgtg ttggatgaaa
     4621 atggcttcga ggcggccggt caggccagcg agcggtgctg cctcgacgaa atcggcggca
     4681 aatcgcggat acgcagccaa gctgttgaaa tttctggtac gccca
//
LOCUS       DASYCS010000154         3073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2643201,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000154 DASYCS010000000
VERSION     DASYCS010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2643201"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1262)
                     /gene="miaB"
                     /locus_tag="VGM46_05005"
     CDS             complement(<1..1262)
                     /gene="miaB"
                     /locus_tag="VGM46_05005"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009449326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEY4191981.1"
                     /translation="MTALATDAGSPSEAIAPQATKKVFVKTYGCQMNVYDSQRMTDAL
                     VADGYSPTDAIGEADLVLLNTCHIREKAAEKVYSELGRIRDMKAERALVGKTLTIGVT
                     GCVAQAEGAEIIRRSPAVDLVVGPQTYHRLPEVLARVRNGGKVVETEYALEDKFEHLP
                     QPRRAEIIRRGVTAFLTVQEGCDKFCTFCVVPYTRGSEVSRPVSQIVEEAEKLAQAGV
                     REVTLLGQNVNAWHGEGAGQGGKNAEWGLGRLLFRLAEIPGIARLRYTTSHPRDMDDE
                     LIAAHRDLDVLMPYLHLPVQSGSDRILKVMNRRHTASDYLKLLDRVRAARADIALSGD
                     FIVGFPGETDADFEATMQLVRDVGYASAFSFKYSPRTGTPGAEMDGHVPEPVKDERLQ
                     RLQALLVEQQQAFTVGMVGKAMPVLIEKP"
     gene            complement(1318..2127)
                     /locus_tag="VGM46_05010"
     CDS             complement(1318..2127)
                     /locus_tag="VGM46_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006337991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY4191982.1"
                     /translation="MIVRIRIFFALAFIVASTLVLAPLQVLAMKTGLWRETVILKLWH
                     RALLKGFGIRVHTLGALSEKRPLLVASNHISWTDILVLGAVVDVNFIAKSELEGWPLL
                     GWLSKLQRTVFIERERKRKSGDQASEIATRLGKGDAMVLFAEGSTGDGNILLPFKSTL
                     FGAASMAISEGAAKEVFIQPVAIAYTRLHGVAFGRHDRGISAWIGDEDLLPHMWALLG
                     SRALDVEVHFGEPVVFSQGSNRKETARLVERRVQGMMETALRDPRPSRPAK"
     gene            complement(2135..2629)
                     /gene="rimI"
                     /locus_tag="VGM46_05015"
     CDS             complement(2135..2629)
                     /gene="rimI"
                     /locus_tag="VGM46_05015"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015314021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEY4191983.1"
                     /translation="MRIPFLSAGPKDFALEPLAMADSDALSVLHREDFVRPWTGAEFS
                     TLLAQDTVFGYGVREVGHGAKAPVGFVLARLAAGEAEILTVAVARSHRRQGLGWRLMD
                     AVLRELHAQRAEALFLEVDETNAPAIALYRRLGFREVGKRANYYQSTERGPNGALVMR
                     RDLQ"
     gene            complement(2629..>3073)
                     /locus_tag="VGM46_05020"
     CDS             complement(2629..>3073)
                     /locus_tag="VGM46_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEY4191984.1"
                     /translation="GLALALKIPAIGVTTLEALAQQARQAFPGRAVLVALDGGRDEIH
                     AALYDEAAILRYGPAVATMAEMVEMASTNKPVLAGTAAMKIADAATGPFNGGPFDIGT
                     TDATSDIAVYARLAATKSTGEKPHPVYLRAADAKPQAGFVLPRIS"
BASE COUNT          548 a         1020 c          962 g          543 t
ORIGIN      
        1 ggcttttcga tcagcaccgg catcgccttg ccgaccattc caacggtgaa ggcctgctgt
       61 tgttccacca gcaacgcctg caacctttgc agccgctcat ccttgacggg ttccggcaca
      121 tggccgtcca tctcggcgcc cggcgtgccg gtgcgcggcg aatatttgaa ggaaaaggcg
      181 gaggcataac cgacatcgcg gacaagctgc atcgtcgctt cgaaatcggc atccgtctcg
      241 ccgggaaagc cgacgataaa gtcgccggac agggcaatgt cggcgcgggc ggcgcgaacg
      301 cggtcgagca gcttcagata atcgctggcg gtatggcggc ggttcatcac tttcaggata
      361 cggtccgacc ccgattgcac cggcaggtgc agatagggca tcagcacatc caggtcgcga
      421 tgcgcggcaa tcagttcgtc gtccatgtcg cgcggatggc tggtggtgta gcgcaggcgc
      481 gcaatgcccg gaatttcggc cagccgaaac agcaggcggc caaggcccca ttcggcgttc
      541 tttccccctt ggccagcccc ctcgccgtgc caggcgttga cgttctggcc aagcagcgtc
      601 acctcgcgca cgccggcctg cgccagcttc tcggcctctt ccacgatctg gctgaccggg
      661 cgcgagactt cggagccgcg cgtatagggc accacgcaga aggtgcagaa cttgtcgcag
      721 ccttcctgca cggtgaggaa ggcggtgacg ccgcgccgga tgatctcggc gcgcctcggc
      781 tgcggcaggt gttcgaactt gtcttccagc gcatattcgg tctcgacgac cttgccgcca
      841 ttacgcaccc tggccagcac ttccggcagg cggtggtagg tctgcgggcc gacgaccaga
      901 tcgacggcgg gagagcggcg gatgatctcg gccccttccg cctgcgccac gcagccggtg
      961 acgccgatgg tcagcgtctt gccgaccagc gcgcgctcgg ccttcatgtc gcggatgcgg
     1021 ccaagctcgg aatagacctt ttcggccgcc ttctcgcgga tatggcaggt gttgagcagc
     1081 accaggtcgg cctcgccgat ggcatctgtc ggcgaatagc cgtcggcgac cagcgcatcg
     1141 gtcatgcgct gcgaatcata gacgttcatc tggcagccat aggtcttcac gaagaccttc
     1201 ttcgtggcct gcggcgcaat agcttcactc ggcgagccgg cgtccgtggc cagcgctgtc
     1261 atatccagtt ccatgcggcg gcttctaacg cctttccatg acaaaaaaca gacgattcta
     1321 ttttgcgggc cggctggggc gcggatcgcg cagcgccgtc tccatcatgc cctgcacgcg
     1381 gcgctcgacc agccttgccg tttccttgcg gttcgagcct tggctgaaca ccaccggctc
     1441 gccgaaatgc acctcgacat ccagcgctcg agaacccagc agcgcccaca tatgcggcaa
     1501 caggtcctcg tcgccgatcc aggcggaaat gccgcgatcg tggcggccga aggcaacgcc
     1561 atgcaggcgc gtataggcga ttgccaccgg ctggatgaac acttccttgg ccgcaccttc
     1621 gctgatcgcc atcgaggccg cgccaaacag tgtgctcttg aacggcaaaa ggatgttgcc
     1681 gtcgcccgtc gaaccttcgg caaacagcac catggcgtcg cccttgccca gccgggtggc
     1741 gatttcgctg gcctggtcgc ccgacttgcg cttgcgctcg cgctcgatga atacggtgcg
     1801 ttgcagcttc gacagccagc cgagcagcgg ccagccctca agctcggact tggcgatgaa
     1861 gttcacatcg accacagcgc ccaaaacgag gatgtcggtc caggaaatgt ggttggaggc
     1921 gaccagcagc gggcgttttt ccgacaaggc gccgagcgtg tgaacgcgga tgccgaaccc
     1981 cttcagcaag gcgcgatgcc acagcttcag gatgaccgtc tcgcgccaaa ggccggtttt
     2041 catcgccagc acctgtagcg gggcgagaac gagcgtgctg gcgacaatga acgccagggc
     2101 gaagaaaatg cgtatccgta cgatcatcat gcgcctattg caggtcgcgg cgcatgacca
     2161 gcgcgccatt ggggccacgc tcggtcgact ggtagtaatt ggcgcgcttg ccgacttcgc
     2221 ggaagccaag ccgcctgtaa agcgctatgg ccggcgcgtt ggtctcgtcc acctcaagga
     2281 aaagcgcctc ggcgcgctgg gcatgcagtt cgcgcagcac cgcatccatc agccgccagc
     2341 caagcccctg cctgcgatgc gagcgggcga ccgcgacggt caatatttcc gcctcgcccg
     2401 ccgcaagcct ggccagcacg aagccgacgg gagccttggc gccgtggccg acctcgcgca
     2461 cgccatagcc gaagacggtg tcctgtgcga gcagggtgga aaactcggcc cccgtccatg
     2521 gccgcacgaa atcctcgcgg tgcagcacgg agagcgcatc gctgtcggcc atcgccagcg
     2581 gttccaaagc gaaatccttc gggcctgccg aaaggaacgg gatccgcatc acgaaatcct
     2641 tggcagcacg aagccggcct gcggcttggc gtcggcggcg cgcagataga ccggatgggg
     2701 tttttcgccg gtgcttttgg tcgcggcaag ccgggcatag acggcgatgt ccgatgtcgc
     2761 atcggtcgtg cctatgtcga atggaccgcc attgaatggg ccggttgcgg catcggcaat
     2821 cttcattgcc gccgtgccgg ccaataccgg tttgttcgtg gaagccattt cgaccatttc
     2881 cgccatcgtc gcgaccgccg gaccatagcg caaaatcgcc gcttcgtcgt acagcgcggc
     2941 atggatttcg tcacgcccgc cgtcgagcgc caccagcacc gcacggcctg gaaaagcctg
     3001 ccgcgcctgt tgcgccagcg cttccagcgt ggtcacgccg atcgccggta tcttcagcgc
     3061 cagcgccagc cct
//
LOCUS       DASYCS010000155         7188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1339550,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000155 DASYCS010000000
VERSION     DASYCS010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 7188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7188
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1339550"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1123
                     /locus_tag="VGM46_05025"
     CDS             <1..1123
                     /locus_tag="VGM46_05025"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEY4191985.1"
                     /translation="ALLDLEKEHVFRNHWQIAGHVSDVPNAGDYLTMDVVGERALIVR
                     GKDGVVRAFNNMCRHRGSRVVADNQGNCKNALVCPFHGWVYNLDGTLRGAARPASFPE
                     LDKSEFGLVPLDLEIWMGFVFIRFHKGGPQLSVAELLKPVAAEMAHYRAEDMVPSTGI
                     WSQTTPVNWKSVRDVDNEGYHVAMAHPALQDLYGASYFDEPFVDGVSRSFATYNPHAG
                     RRWSVRQYVKIAPEQERLPEYLKKAWVYYGIFPNAVISVMPESVQFYQEFPVSTGKTL
                     LRGGIYRYRDESREQAAARYLSNRIDRDTMSEDVQLTIWSNESMLSNAFAGFYLSDLE
                     YGVRTHHDHLRAMLPVLNLQTAPDEKEMAGINDGLKADG"
     gene            complement(1148..2053)
                     /locus_tag="VGM46_05030"
     CDS             complement(1148..2053)
                     /locus_tag="VGM46_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY4191986.1"
                     /translation="MTRTFSTLIGFSAILTWSFLALLSTAAGAIPPFQLAAMAFFLGG
                     LVGASSWLFRPQAIKSLRQPWQVWALGVAGLCIYHCAYFFAIQSAPPVEASLIAYLWP
                     LLIVVFAAFLPGERLRAHHLLGVLLGLAGAIIVITKGGSVGLADGIKPGHIIALFCAF
                     IWSGYSVLSRRFGAVPTDVVAGYCLITAAVALVLHLALEPTVVPQTATQWAAIAVLGT
                     IPLGAGFYAWDWGCKHGDIMVLGALSYAAPLFSTVVLLAAGYGAYHWSVALACGLIIA
                     GALIAAKDMLFRRSNPAKPAASQTT"
     gene            2375..3256
                     /locus_tag="VGM46_05035"
     CDS             2375..3256
                     /locus_tag="VGM46_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007605775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY4191987.1"
                     /translation="MRARDIASYLFLAVAWGLSFLVLVKVVGAFGWAGAVTLRCLLAS
                     ATLMAMAKALRGELRFSLGWRPYAVVGATTVAGQLVGLSYGTPLIGTAMSAILVAAIP
                     LFSMVISQLWGLERMTAPRLAGLLLGMAGIVLLVGFPAVPVTPSFLLGCAACLFASFS
                     AAFGSNYASLHLRTAGSLEVAAGSFLAGGLMTLPLLLAVPVPAMPGPVDFLYLVVLGC
                     VMSALTYATYFRLVTAIGPTRAISVEFAVTVVAVLVGAGVLGEPLTPIQIVGAAVIVA
                     GCMLVLGVLPARFGKRG"
     gene            complement(3269..4708)
                     /locus_tag="VGM46_05040"
     CDS             complement(3269..4708)
                     /locus_tag="VGM46_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HEY4191988.1"
                     /translation="MAAARDIQTSGRGNREDGQGGCPAGDAVELATRHLVQPWPYAGS
                     VGAEARTLIGEGDGIYITDADGKRLIDGPAGMWCTNVGHRREELAKVMYDQAMALSYN
                     TPWYTMNAPSAELARRIAGHAPGDLGHVFFTTGGSTAVETALRFMQFFNNVKGRPEKK
                     LILSRGGAYHGSTYLSASLNGRARDRDWMDGVDGLVVKLSSPDPFRRPKGMSVEAFGD
                     FLADEFRDTVARLGADRIGAFVGEPVQASGGVIVPPKNYLKRIREICRANDILYVSDE
                     VVTAFGRLGSVFASGDVFGVDPDMIVFAKGVTSGYFPLGGIVISEALLEELRRSNHPD
                     ALFGHGLTYTSHPVGCAVALKNLDLLEQGLLAHTQAVAPYFQSRLKTLEDLPLVGEVR
                     GIGMMACVECVADRDSLDPLELDRDVGARIDAHCQELGLLVRPLINMCVMSPPLVITR
                     SQIDDLVGILREGISRTMDDLRKEGVWNG"
     gene            5054..5935
                     /locus_tag="VGM46_05045"
     CDS             5054..5935
                     /locus_tag="VGM46_05045"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4191989.1"
                     /translation="MTRARRNVLLQSEAVQGLSLISPTLIYALVLLVVPILVVIAHSF
                     WTQHYLTIDRTFTLENYRVALTEPIYRDLLWRSLYISMTVSLLTVVLAYPIAYYISFH
                     GGQRKGLWLFLITIPFWTSYLLRVMAWKVILGYNGVLNSGLMGLGITDHPTTALLYNS
                     SAVIITLTHAWAAFAVLPIFVSLEKVDRTLVEAAKDLGDGPLRSFLRVTLPLSMPGVI
                     SALLIVMIPTVGDYVTPKLVGGKDGVMIANAIQGLFGKGANWPLGAALSVTTMLIVTA
                     MAGAAVFILRSATRLAR"
     gene            5966..6793
                     /locus_tag="VGM46_05050"
     CDS             5966..6793
                     /locus_tag="VGM46_05050"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4191990.1"
                     /translation="MKGLRRFMPGGSWLGAYALLYVVFLYLPVIFLPIFSVNTAATPK
                     FPLSGFTWKWYEQLPHTPALLDAAWNSLIVGVSASVLATTFGILAARAITRYRFPGRR
                     PINGLIMAPLVLPEVIIAISMLLVMLQLGLSLSLFTVVLGHILICIPYSMTVLTSGFE
                     GFDRSLEEASADLGESAFGTFRRVTLPMVAPAIISSLLVSFTISLDEFIIAFFLTGTE
                     ATLPIYIWGQLRFAAKLPGVLALGTLLLVASFVLMTLAEVLRRRAARRANTEGGLYA"
     gene            6831..>7188
                     /locus_tag="VGM46_05055"
     CDS             6831..>7188
                     /locus_tag="VGM46_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006201239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4191991.1"
                     /translation="MIEISHVTRSYGVFKALDDANVTIREGEFFSLLGPSGCGKTTLL
                     RMIAGFDNPTSGSISVDGQPMEGIPANRRPTNMVFQSYAIFPHLNVEQNVAYGLKRLK
                     LEAAEEKRRVEEALAQV"
BASE COUNT         1209 a         2234 c         2384 g         1361 t
ORIGIN      
        1 cgcccttctc gacctcgaaa aggagcatgt cttccgcaat cactggcaga tagccggcca
       61 tgtttcagac gtgccgaacg cgggcgatta cctgaccatg gatgtggtcg gcgaacgcgc
      121 cctgatcgtg cgcggcaagg acggcgtggt gcgggccttc aacaatatgt gccggcatcg
      181 cggcagccgc gtcgttgccg acaatcaggg caattgcaag aacgcgctgg tctgcccgtt
      241 tcacggctgg gtctacaatc tcgacggcac gctgcgcggc gcggcaaggc cggcatcgtt
      301 tcccgaactg gacaagagcg agttcggcct ggtgccgctc gaccttgaaa tctggatggg
      361 cttcgtcttc atccgttttc acaagggcgg gccgcagctt tccgtcgccg agcttctgaa
      421 gcccgtcgcg gcggaaatgg cgcattacag ggccgaagat atggtgcctt cgaccggcat
      481 ctggagccag acaacgccgg tgaactggaa atcggtgcgc gatgtcgata atgaaggcta
      541 ccacgtcgcc atggcgcatc cggccttgca ggacctttat ggcgcgagct atttcgacga
      601 gccgttcgtc gatggcgtgt cgcgttcctt cgccacctat aatccgcatg ccggccgccg
      661 ctggagcgtc aggcaatatg tcaagatcgc gccggagcag gagcggttgc ccgaatatct
      721 gaagaaggcg tgggtctatt acggcatctt tccgaacgcg gtgatctcgg tgatgccgga
      781 atcggtgcag ttttatcagg aattcccggt ttcgaccggc aagacgctgt tgcgcggcgg
      841 catctaccgc taccgcgacg aaagccgcga gcaggcggcg gcgcgttatc tgtccaatcg
      901 catcgaccgc gataccatgt cggaagacgt gcaactgacg atctggtcga acgaatccat
      961 gctgtcgaat gcctttgccg gcttctacct gtccgacctc gaatatggcg tgcgcaccca
     1021 tcacgaccat ctgcgcgcca tgctgccggt gctgaacctt caaaccgcgc cggacgagaa
     1081 ggaaatggcg gggatcaacg acgggctaaa agcggacggg taagggcgcg ggcttttcgc
     1141 agcgcggtca ggtggtctgc gaggccgcgg gctttgccgg atttgagcgc cgaaacagca
     1201 tgtccttcgc cgcgatcagc gcgccggcga tgatgaggcc gcaggcgagc gcgaccgacc
     1261 agtgataggc gccatagccg gccgccagca ggacgacggt ggagaaaagc ggcgcggcat
     1321 aagacagcgc gccaagcacc atgatgtcgc cgtgcttgca accccagtcc caggcataga
     1381 agccggcgcc aagcggaatg gttcccaaaa cggcgatggc cgcccattgg gtggcggtct
     1441 gcggcaccac ggtcggctcc agcgcgagat ggaggacaag ggcgacggcg gcggtgatca
     1501 ggcagtagcc ggccaccaca tcggtcggca ccgcgccgaa gcggcgcgac aggacggaat
     1561 agccggacca gatgaaggcg caaaacagcg cgatgatgtg gccgggcttg atgccgtcgg
     1621 ccagcccgac gctgccgccc ttggtgatga cgatgatcgc gccggccaga ccgagcagca
     1681 cgccgagaag atggtgggcg cgcagccttt cgccgggcag gaaggccgcg aagacgacga
     1741 tcagcagcgg ccacagatag gcgatcaggc tggcttccac cggcggcgcc gactggatgg
     1801 cgaagaaata ggcgcagtgg tagatgcaca ggccggcgac gccgagcgcc cagacctgcc
     1861 agggctggcg cagcgatttg atggcctgcg ggcgaaacag ccagctcgat gcgccgacca
     1921 gcccgcccag gaaaaaggcc atggctgcca gctgaaaggg cgggatcgcg ccggccgccg
     1981 tcgacagcag cgccaggaac gaccaggtca ggatggcaga aaagccgatc agggtggaga
     2041 aggttctggt catcgacgca ccggcgtgag ttgtctcctg cgtttggcac atcatcccgg
     2101 tggttggcta tccctgccgc agcgccggtt gtcggaagag cgacatggtt cggcttcgtg
     2161 atgagcgcgc gccgatagtg gccgaacgcg ggaagtcgcg gcgggcgacg tccgaccaac
     2221 cgtaagggcg cgcaaggcaa ggactggctg tccataccat tcggttgcga gtgtcgcgcc
     2281 atgctctgtc catcggggct ctatccattg ggggcgattg atcgtaggag cgcgcggcgg
     2341 cgttgctgcc gcagccaagg ggttcggcgc ttccatgcgt gcacgcgata tcgcttccta
     2401 cctgtttctg gccgtggcct ggggcttgtc ctttctcgtg ctggtcaagg tggttggcgc
     2461 gttcggctgg gcgggggcgg ttacgctgcg ctgcctgctg gcgagcgcca cgctgatggc
     2521 gatggcgaag gcgttgcggg gcgaactgcg cttcagcctc ggctggcggc cctatgcggt
     2581 ggtcggcgcg acgacggtgg cggggcaact ggtggggctt tcctatggca cgccgcttat
     2641 cggcaccgcc atgtcggcga tattggtggc ggccattccg ttgttttcga tggtaatcag
     2701 ccagctctgg gggctggagc ggatgacggc gccccggctg gccggcctgc ttctgggcat
     2761 ggccggcatc gtgcttctgg tcgggtttcc ggccgtgcca gtaacgcctt cattcctgct
     2821 tggctgcgca gcgtgcttgt tcgcatcgtt ctcggcggcc ttcggcagca attatgccag
     2881 cctgcacctg cgcaccgccg gctcgctgga agtcgcagcc ggttcgttcc tggccggcgg
     2941 gctgatgacg ctgccgcttt tgcttgcggt tcccgtcccg gccatgccgg ggccggtgga
     3001 ttttctctac ctcgtcgttc tgggctgcgt gatgagcgcg ctgacctacg ccacctattt
     3061 ccggctggtg acggcgatcg ggccgacgcg ggcgatcagc gtcgagttcg cggtgacggt
     3121 ggttgccgtg ctggtgggcg cgggcgtgct cggggaaccg ctgacgccga tccagatagt
     3181 cggcgccgcc gtcatcgtcg ccggctgcat gctggtgctg ggcgtgcttc cggcgcggtt
     3241 tggaaagcgc ggctgaccgc cattgccgtc agccgttcca gacgccttcc ttgcgcaggt
     3301 cgtccatggt gcgtgaaatg ccttcgcgca ggatgccgac gagatcgtcg atctggctgc
     3361 gggtgatcac cagcggcggc gacatcacgc acatgttgat gagcgggcgc accagcaggc
     3421 ccagttcctg gcagtgcgcg tcgatacgcg cgccgacatc gcgatccagc tccagcggat
     3481 cgagactgtc gcggtcggca acgcattcga cgcaggccat cattccgatg ccgcgcacct
     3541 cgccgaccag cggcaaatct tcgagcgtct tcaaccgcga ctggaaatag ggcgcgaccg
     3601 cctgcgtgtg ggcaagcaag ccctgctcca gcaggtcgag gttcttcagc gccacggcgc
     3661 agcctaccgg gtggctggta taggtaaggc catggccgaa cagcgcgtcg ggatggttcg
     3721 aacggcgcag ttcttcgagc agcgcctccg aaatgacgat gccgccgagc gggaaatagc
     3781 cggaagtgac gcccttggcg aagacgatca tgtcggggtc gacgccgaac acatcgcctg
     3841 aagcaaagac ggagccgagc cgcccgaagg cggtgaccac ttcgtccgac acgtagagga
     3901 tgtcattggc gcggcaaatc tcgcggatgc gcttcaggta gtttttcggc ggcacgatga
     3961 cgccgcccga agcctgcacc ggctcaccga cgaaagcgcc gatcctgtcg gcgccaaggc
     4021 gggcgaccgt gtcgcggaat tcgtcggcga gaaaatcgcc gaaggcctcc acgctcatgc
     4081 ccttggggcg gcggaacgga tcgggcgagg ataatttgac gacgaggccg tcgacgccgt
     4141 ccatccagtc acggtcgcgg gcgcggccgt tcagcgaggc cgacaaatag gtggacccat
     4201 gataggcgcc gccgcgcgac aggatcagct tcttttccgg gcggcctttg acgttgttga
     4261 agaactgcat gaagcgcagc gccgtctcca ccgcggtcga gccgccggtg gtgaagaaca
     4321 catggccaag gtcgccgggc gcgtggccgg cgatgcggcg ggcaagctcg gcggacggcg
     4381 cgttcatcgt gtaccagggc gtgttgtagg acagcgccat ggcctggtcg tacatgacct
     4441 tcgccagttc ctcgcggcgg tggccgacat tggtgcacca catgccggcc gggccgtcga
     4501 tcagccgctt gccgtcggcg tcggtgatat agatgccgtc gccctcgccg atcagggtgc
     4561 gcgcttccgc gccgaccgaa cccgcataag gccagggctg gacgaggtgg cgggtggcaa
     4621 gctcgacggc atcgccggcc gggcagccgc cttggccgtc ttccctattt ccccggccgc
     4681 tcgtttgaat gtctctcgcc gccgccattg ctcgcctcgc gctttcaagt ggattcaacg
     4741 gaccggagcc acgcgccgga atttgcaaac cgccgcttat ttcacttcat tttgaatgtc
     4801 cgttcaatca atattgcaga attaacgctt gatttcaatt caccaatgcg ctcatgatga
     4861 aagaaagccg gcgcgggaac agccggtcca tgatgggtgg cagcatggcg gaaacgttca
     4921 gaccggcgac cggcaacccg cctgggatgg agccaacggc tcgtcgccat ccgcgccttc
     4981 ggcatccggc ccttcggcat agtggcggcg tcctgcaatg acggcgctgg cgcagccgcc
     5041 ctcacggcat cgcatgacgc gggcgcgccg caatgtcctt ttgcaatccg aagccgttca
     5101 gggcctgtcg ctcatcagcc cgaccctgat ttacgcgctg gtgctgttgg tggtgccgat
     5161 cctggtggtg atcgcccatt cgttctggac gcagcattac ctgaccatcg accgcacctt
     5221 cacgctggag aactaccgcg tcgcgctgac cgagccgatc taccgcgatc ttctgtggcg
     5281 ctcgctctat atttcgatga cggtcagcct gttgacggtc gtgctggctt accccatcgc
     5341 ctactacatt tccttccatg gcggccagcg caaagggctg tggctgttcc tcatcaccat
     5401 cccgttctgg accagctacc tgctgcgcgt catggcgtgg aaggttatcc ttggctataa
     5461 tggcgtgctc aattccggcc tgatgggtct cgggatcacc gaccatccga cgacggcgct
     5521 gctttacaat tcaagcgcgg tcatcatcac gctcacccat gcctgggcgg cgtttgccgt
     5581 attgccgatc ttcgtctcgc tggaaaaggt cgaccgcacg ctggtcgagg cggccaagga
     5641 tctgggcgac gggccgctgc gcagcttcct gcgggtgacg ctgccgctgt cgatgccggg
     5701 cgtgatttcg gcgctgttga tcgtgatgat cccgacggtc ggcgattatg tgacgccgaa
     5761 gcttgtcggc ggcaaggacg gcgtgatgat cgccaacgcc atacaggggc tgttcggcaa
     5821 aggcgccaac tggccgctgg gtgcagcact ttccgtcacc actatgctga tcgtcacggc
     5881 gatggcgggc gctgcggtgt tcatcctgcg ctccgcaaca aggttggcgc gatgaggagg
     5941 gtggcgcgat gaggaggttg gcgcgatgaa gggtcttcgc cgcttcatgc ccggcggaag
     6001 ctggctcggc gcctatgcgc tgctctatgt cgtgttcctg tacctgccgg tgatcttcct
     6061 gccgatcttc tcggtcaaca cggcggcgac gccgaaattt ccgctctcgg gcttcacctg
     6121 gaaatggtac gagcagctgc cgcatacgcc ggccctgctg gacgccgcat ggaacagcct
     6181 tatcgtcggc gtttcggcct cggtgctggc gacgacattc ggcattctgg ctgcgcgcgc
     6241 catcacccgc taccgctttc ccggccgccg cccgatcaat ggcctgatca tggcgccgct
     6301 ggtgctgccg gaagtcatta tcgccatctc catgctgctg gtgatgctgc aactgggcct
     6361 cagcctgtcg ctgtttaccg tcgtgctcgg ccatatactg atctgcattc cctattcgat
     6421 gacggtgctg acctcgggct tcgagggctt cgaccgcagc cttgaagagg cttccgccga
     6481 tcttggcgaa agcgccttcg gcaccttccg gcgtgtcacg ctgccgatgg tggcgccggc
     6541 catcatttcc agcctgcttg tttccttcac catctcgctg gatgaattca tcatcgcctt
     6601 cttcctcacc ggcacggagg cgacgctgcc gatctacatc tggggccagt tgcgctttgc
     6661 cgccaagctg ccgggcgtgc tggcgctggg cacgctgctg cttgtcgctt cgttcgtgct
     6721 gatgacgctt gccgaagtgc tgcgccgccg cgcggcccgc cgcgccaata ccgaaggggg
     6781 cctctatgcc tgaacagcag acaccggaac ggtcggggtc cggccgggcg atgatcgaaa
     6841 tcagccacgt cacgcgcagc tatggcgtgt tcaaggcgct ggacgacgcc aatgtgacga
     6901 tccgggaagg cgagttcttt tcgctgctgg ggccttccgg ctgcggcaag accacgctgc
     6961 tgcgcatgat cgccggtttc gacaacccga ccagcggttc catttcggtc gatggccagc
     7021 cgatggaagg cattccggcc aaccgccgcc cgaccaacat ggtgttccag agctatgcga
     7081 tctttccgca tctcaatgtc gagcagaacg tcgcctatgg gctgaagcgg ctgaagctcg
     7141 aagcggcgga ggaaaagcga cgcgtcgagg aagcgctggc gcaggtgt
//
LOCUS       DASYCS010000156         5127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3802284,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000156 DASYCS010000000
VERSION     DASYCS010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 5127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5127
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3802284"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2405)
                     /gene="ppdK"
                     /locus_tag="VGM46_05060"
     CDS             complement(<1..2405)
                     /gene="ppdK"
                     /locus_tag="VGM46_05060"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010914919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEY4191992.1"
                     /translation="MTKWIYTFGDGAAEGRASDKNLLGGKGANLAEMCSLGLPVPPGF
                     TITAEMCNWYYANGQAFPSSFAGEVDVALDHIGRLTGRNFGDPEKLLLVSVRSGARAS
                     MPGMMDTVLNLGLNDRTVEALASDSGDARFAYDSYRRFIQMYSDVVMGLDHEVFEEIL
                     EDQKASLGHELDTGLTAAEWQGVIGLYKAKVEEELGKPFPQDPREQLWGAIGAVFSSW
                     MNARAITYRRLHDIPESWGTAVNIQAMVFGNMGETSATGVAFTRNPSTGEKQLYGEFL
                     VNAQGEDVVAGIRTPQNITEAARIAAGSDKPSLQKLMPDAFDAFVAISGKLEQHYRDM
                     QDLEFTIERGKLWMLQTRSGKRTAKAALRIAVEMAQDGLITQDEAVARIDPASLDQLL
                     HPTIDPEAARDVIGVGLPASPGAATGEIVFSSDDAEELKKQGRKAILVRIETSPEDIH
                     GMHASEGILTTRGGMTSHAAVVARGMGKPCVSGTGSLRVDYKAGTMTARGIVLKKGDI
                     VTIDGSTGQVLKGAVAMLQPELSGDFAAIMEWADKARRMKVRTNAETPADARMARSFG
                     AEGIGLCRTEHMFFDGDRIIAMREMILADTETDRRAALAKLLPMQRSDFLELFEIMAG
                     LPVTIRLLDPPLHEFLPKTEEEIAEVAAVMNVSADKLRQRTEALHEFNPMLGHRGCRL
                     AVSYPEIAEMQARAIFEAAVEAGRKAGALVVPEIMVPLVGIEKEFDYVKAGIDAVAKS
                     VMEETGVKIEYLTGTMIELPRAAIRADVIARTAEFFSFGTNDLTQTTFGISRDDAASF
                     LET"
     gene            2625..2969
                     /locus_tag="VGM46_05065"
     CDS             2625..2969
                     /locus_tag="VGM46_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006206308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY4191993.1"
                     /translation="MKQAGKLDYLEMPATGGTLDRVKAFYSAAFSWSFTDYGATYSAF
                     DEGLEGGFQADGAEASSRPLPVLYSENIEESLNAVETAGGTILRPIFSFPGGRRFHFA
                     DPAGNELAVWGK"
     gene            3305..3379
                     /locus_tag="VGM46_05070"
     tRNA            3305..3379
                     /locus_tag="VGM46_05070"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3337..3339,aa:Gln,seq:ttg)
     gene            complement(3498..>5127)
                     /locus_tag="VGM46_05075"
     CDS             complement(3498..>5127)
                     /locus_tag="VGM46_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006330665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEY4191994.1"
                     /translation="LWRWDGFTASADAPTAAALRLAQKNRLAELDAEAVEATRKLRAA
                     EEILARAETALRFASEAEREARQGWRDAQHTLDAARSALAQAERATGELSSRRAALDE
                     ALARVADSHEEAAAAFIEAETLLQDAADLGDLQARLEQAAAHVSHERSALADARALYD
                     GLKREAEARLRRLEAIGAERRNWLARAENASVQIASLGERKAEAEAERENLADAPEQI
                     DERRRLLLSQIAEAERLRKAASDRLAEAETQQAGFDKAAIAAIQGLAEAREARARAEE
                     RLTAADERRAEVEARIQETLNTPPHLVIRHTGLEADSPLPDVADVERQLERLKIERER
                     LGAVNLRAEEEQQEMSSRLEAIVSEREDIIEAIRKLRQAIQSLNREGRERLLAAYDVV
                     NGHFQRLFSHLFGGGTAELQLIESEDPLEAGLEILARPPGKKPQTMTLLSGGEQALTA
                     MSLIFAVFLTNPAPICVLDEVDAPLDDQNVERFCNLMDEMAASTETRFVVITHNPITM
                     ARMDRLFGVTMAEQGVSQLVSVDLQAAEAMREAS"
BASE COUNT          888 a         1645 c         1602 g          992 t
ORIGIN      
        1 tatgtctcca ggaaagaggc ggcgtcgtcg cgcgagatgc cgaaggtggt ctgcgtcaaa
       61 tcattggtgc cgaatgaaaa gaattcggcg gtcctggcaa tgacatcggc gcggatggcg
      121 gcgcgcggca gttcgatcat cgtgccggtg aggtattcta tcttgacacc ggtttcctcc
      181 atcacgcttt tggcgaccgc atcgatgcct gccttgacgt agtcgaactc cttctcgatg
      241 ccgaccagcg gcaccatgat ttccggcacc accagcgcgc cggccttgcg gcccgcctcc
      301 accgccgcct cgaaaatggc gcgcgcctgc atttccgcga tctcgggata ggacaccgcc
      361 agacggcagc cgcgatggcc gagcatcggg ttgaactcgt gcagggcttc ggtgcgctgg
      421 cgcagtttgt cggccgaaac gttcatgacg gcggcgactt cggcgatttc ttcctctgtc
      481 ttgggcagga attcgtgcag cggcgggtcg agtaggcgga tcgtcaccgg caagccggcc
      541 atgatctcga acagttcgag gaaatccgaa cgctgcatcg gcaacagctt ggcaagggct
      601 gcgcgccggt ccgtctccgt gtcggccagg atcatctcgc gcatggcgat gatgcggtcg
      661 ccatcgaaga acatgtgctc ggtgcggcaa agcccgatgc cctcggcccc gaaggagcgc
      721 gccatgcgcg catcggccgg cgtctcggca ttggtgcgca ccttcatgcg gcgcgccttg
      781 tcggcccact ccatgatggc ggcgaaatcg cccgacagtt ccggctgaag catcgccacc
      841 gcgcccttca gcacctggcc ggtgctgccg tcgatggtaa cgatatcgcc ttttttgaga
      901 acgatgccgc gcgccgtcat cgtgcccgcc ttatagtcca cccgcaacga gccggtgccc
      961 gacacgcacg gcttgcccat gccgcgcgcc accaccgccg catggctggt catgccgccg
     1021 cgcgtggtga ggatgccttc cgaagcgtgc atgccgtgaa tatcctcggg gcttgtctcg
     1081 atgcgcacga gaatggcctt gcgcccctgc ttcttcagtt cttccgcatc gtcggaggaa
     1141 aacacgatct cgccggtggc cgcgccgggc gacgccggaa ggccgacgcc gatgacatcg
     1201 cgcgccgctt ccgggtcgat cgtcgggtgc agcaactgat ccagcgacgc cggatcgatg
     1261 cgggcgaccg cctcgtcctg cgttatcagg ccgtcctgcg ccatctcgac ggcgatgcga
     1321 agggcggctt ttgcggtgcg cttgccggaa cgcgtctgga gcatccacaa tttgccgcgc
     1381 tcgatggtga attcgaggtc ctgcatgtcg cggtaatgct gctccagctt gccggagatc
     1441 gccacaaacg catcgaatgc atccggcatc agcttttgca gcgacggctt gtcggaaccg
     1501 gcggcgatgc gcgcggcttc ggtgatgttt tgcggcgtgc ggatgccggc caccacgtcc
     1561 tcgccctgcg cattgaccag aaattcgccg tagagctgct tctcgccagt ggaaggattg
     1621 cgggtgaagg cgacgccggt ggccgaagtc tcgcccatat tgccgaacac catggcctgg
     1681 atattgaccg ccgtgcccca gctttcggga atgtcgtgca ggcggcgata ggtgatggcg
     1741 cgcgcgttca tccagcttga aaacaccgca ccgatggcgc cccaaagctg ctcgcgcggg
     1801 tcctgtggaa agggttttcc cagttcttcc tcgaccttgg ccttgtagag gccgatcacg
     1861 ccctgccact cggcggcggt cagtccggta tcgagttcat gaccgagcga ggccttctgg
     1921 tcctccagta tttcctcgaa cacttcatga tccagcccca tgaccacatc ggaatacatc
     1981 tggatgaagc gccgatagct gtcataggcg aagcgcgcat cgcccgaatc ggaggcaagc
     2041 gcctcgacgg tccggtcgtt gaggccgaga ttaagcaccg tatccatcat gccgggcatg
     2101 gaagcgcgcg cgcccgagcg caccgacacc agcagcaatt tctcaggatc gccgaaattg
     2161 cggccggtca gccggccgat atgatcgagc gcgacatcga cttcgccagc gaaagaagac
     2221 ggaaaagcct gaccgttggc ataataccag ttgcacattt cggcggtgat ggtgaaaccc
     2281 ggcggcaccg gcaggccaag gctgcacatt tcagccaggt tggcgccctt gccaccgagc
     2341 aggttcttgt cggaagcgcg gccttctgcg gctccgtcgc cgaaagtgta aatccatttg
     2401 gtcatgcatg ctctccaggt ttcggagatg gaaaaagtcc ggcgacaccg aaagttccgc
     2461 catacgccca gatgcgatat gatgctgcaa tgcgaaacgc aaggctccag ccgggcggcc
     2521 gcctcctaca atcgattagg tgacgaactc aaaaaaagta cgggaaaaaa cttgcgcgca
     2581 gaggattgaa tgctatagaa catatcagga acatgaggct tacgatgaaa caggcaggca
     2641 agctcgacta tcttgaaatg ccggccaccg gcggcacgct ggatcgcgtg aaggcgtttt
     2701 acagcgcggc cttttcatgg tcgttcaccg actacggcgc gacctactcg gcattcgacg
     2761 aagggctgga aggcggattt caggccgatg gcgcggaggc ctcatccagg ccgctgccgg
     2821 tgctctattc ggaaaacatc gaggagagcc tgaacgcggt cgaaaccgcg ggcggcacga
     2881 tcctgcggcc gatcttttca ttcccaggcg ggcggcgttt tcatttcgcc gatccggccg
     2941 gcaacgagct ggcggtgtgg ggcaagtagc gtatcggcga ttgccctgcc gcacgatcgg
     3001 cgggcagaag gatgcgccag aacactatag cagttctggt ttttaccgaa tcggtaaaac
     3061 cgctctatcc ctttgttttg gccgcatttc cggacgcaaa accgtttcac acttttgctg
     3121 gaaatgctct gtctcttcgt tttggtcgca tttccggacg caaaaccgtt tcacactttt
     3181 gctggaaatg ctctgatcga acggcgaagc cgcaaacccg ctggaaaggc aagcccaacc
     3241 ggattgtact ttttgtcggc tctgcctata aggccgcctg tatcgattca ggctatcggc
     3301 ctgttggggc gtcgccaagc ggtaaggcaa cggtttttga taccgtcatt cccaggttcg
     3361 aatcctggcg ccccagccaa ttccttcatt atgagccaat ttcttcgtca tgcggtttcg
     3421 ttcggctttg ccggaacctt caaaggcttc ggcgtggcct agggcttagg cgcggcttac
     3481 ggcgatgcgg cctgaaatca gcttgcctcg cgcatggctt cggcggcctg gaggtcgacc
     3541 gagacgagtt ggctaacccc ctgctccgcc atggtgacgc cgaacaggcg gtccatgcgc
     3601 gccatggtga tgggattgtg cgtgatgacg acgaaccgcg tttcggtcga ggccgccatc
     3661 tcgtccatca ggttgcagaa acgctcgaca ttctggtcgt ccagcggtgc atcgacctcg
     3721 tccagcacgc agatcggcgc cggattggtc aggaacacgg cgaagatcag cgacatcgcc
     3781 gtcagcgcct gctcgccgcc tgaaagcagc gtcatggtct gcggcttctt gccgggcggg
     3841 cgggcgagaa tttcaagccc ggcctcaagc ggatcttccg attcgatcag ttgcagttcc
     3901 gccgtgccgc cgccgaacag atgcgagaac agccgctgga aatggccgtt gaccacatcg
     3961 taggcggcca ggagccgctc acggccctcg cggttaaggc tctggattgc ctggcgaagc
     4021 ttgcggatcg cctcgataat gtcctcgcgc tcggaaacga tggcttccag ccggctggac
     4081 atttcctgct gttcctcctc ggcgcgcaga ttgacggcgc caagccgctc gcgctcgatc
     4141 ttcaaccgct caagctggcg ctcgacatcg gcgacgtccg gcagcgggct gtcggcttca
     4201 aggccggtat ggcggatgac gagatggggc ggcgtgttca gcgtttcctg gatgcgcgcc
     4261 tcgacctcgg cgcggcgctc gtcggcggcg gtcagccgct cctcggcgcg ggcgcgcgcc
     4321 tcgcgcgcct cggccagacc ctgaatggcc gcaatggccg ccttgtcaaa cccggcctgc
     4381 tgcgtttccg cttccgccag gcgatcgctt gccgccttgc gcagccgttc agcctcggcg
     4441 atctgcgaca gcaaaagccg gcgccgctca tcgatctgtt ccggcgcgtc ggcgagattt
     4501 tcgcgctccg cttcggcctc cgccttgcgt tcgcccagcg aggcgatctg gacggaggcg
     4561 ttttcggcgc gcgccagcca gttgcggcgc tcggcgccaa tggcctccag ccgccgcagc
     4621 cgcgcctcgg cctcgcgctt cagcccgtca tacagggcgc gcgcatcggc aagggcggaa
     4681 cgttcgtgcg aaacatgggc ggcggcctgc tccagccgcg cctgcaaatc gccaagatcg
     4741 gcggcatcct gcaacagcgt ttccgcctcg ataaaggcgg cggcggcttc ctcatggctg
     4801 tccgccacgc gcgccagcgc ctcgtccaac gcggcgcggc ggctcgacag ttcgccggtg
     4861 gccctttcgg cctgggccag cgcgctgcgg gcagcgtcca gcgtgtgctg cgcgtcgcgc
     4921 cagccctgcc ttgcctcgcg ctccgcctcg ctggcaaagc gcaaagccgt ttcggcccta
     4981 gccagtattt cttcggcggc acgcagcttg cgcgtcgcct cgacggcctc ggcatcgagt
     5041 tcggccagcc ggttcttttg cgcaagacgc aaggcggcgg cggtgggcgc atcggcgctg
     5101 gcggtaaagc catcccagcg ccacagc
//
LOCUS       DASYCS010000157         8097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_5109192,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000157 DASYCS010000000
VERSION     DASYCS010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 8097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8097
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5109192"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1501
                     /locus_tag="VGM46_05080"
     CDS             <1..1501
                     /locus_tag="VGM46_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015319307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEY4191995.1"
                     /translation="ANLWPPAIAILRRETHGNYPAATAILKCVYEGLLVPFDTALRIE
                     QRYFTEIMQTKEAAAMIRSLFISLQELNKGARRPDGVPETKFKKIGVLGAGFMGAGIA
                     YVTAKAGIPVVLLDRDMAAAEKGKAHSEGLMDGLIKKGRATQQDKEKLLALITPTADY
                     ARLAGCDLVVEAVFEDSGVKKAATEQAEAVLKPSAIFASNTSTIPITVLAKNAARPKN
                     FVGIHFFSPVDKMMLVEIILGKKTGDKALATAIDFVRAIKKTPIVVNDTRGFYVNRCV
                     LRYMAEAYKMLIEGVPAAMIENAARAAGMPVGPLALTDETAVDLAQKIMHQTVRDLGD
                     KALDPQQMKLIDTLVDKHQRLGRKNGKGFYDYPQKPAKKKLWPGLRDLYPQKKAEEVD
                     FEELKQRLLATIALEAARVMEEKIVTDPREADVGSILAFGFAPYTGGALSYIDGIGAK
                     KFVALAKGLQKKYGAEFKPPKLLIDMANKGETFYQRFDPYAKGETKKAA"
     gene            1537..2373
                     /locus_tag="VGM46_05085"
     CDS             1537..2373
                     /locus_tag="VGM46_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016736768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEY4191996.1"
                     /translation="MIFDTHLHLIDRSALHYPWLSGAPALNRDFSYEEYSVQARRAGI
                     EAALHMEVDVDPADIEAETGHVKALAAREGSLLKGAIASCRPEDSGFAAYLERQLADP
                     FVKGFRRVLHVVPDDISQGALFRENIGRMAGSRLTFDLVVQPHQIPKAIALADLAPDV
                     HFVLDHCGAPDIKGDGEHPWREHMAEIARRPNVTAKISGVVAYADAQSWSVETLRPYV
                     EHTISAFGWDRVVWGSDWPVCTLGGGLAVWVAATHALLAGVGMADRAKLLSGNARRLW
                     SL"
     gene            complement(2395..2682)
                     /locus_tag="VGM46_05090"
     CDS             complement(2395..2682)
                     /locus_tag="VGM46_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013891978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HEY4191997.1"
                     /translation="MPKGYWIARVDVRDAERYKDYVSTAKPAFERFGAKFLARGGEPD
                     HVEGPGRARNVIIEFESLAIARQCYDSPEYQAAKAIRLTVADGEIVLLEGA"
     gene            complement(2823..3521)
                     /gene="pyrF"
                     /locus_tag="VGM46_05095"
     CDS             complement(2823..3521)
                     /gene="pyrF"
                     /locus_tag="VGM46_05095"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006326262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEY4191998.1"
                     /translation="MNVETMRERLIVGLDVPTVAEAETVVRELGDAVSFYKIGYQLAF
                     AGGLDFARDLAADGKKIFLDMKLLDIDNTVAKGVENIVRMGMTMLTLHAYPKTMQAAV
                     KAAQGSGLCLLAVTVLTSMDEQDVIDAGYEYDPRALVLRRAEQALHAGMGGIVCSASE
                     AEAVRRIVGPDMAVVTPGIRPTGADRGDQKRVVTPGQAIRNGSSHLVVSRPIVGAVDR
                     RGAAQAILDEMRAA"
     gene            complement(3518..4105)
                     /locus_tag="VGM46_05100"
     CDS             complement(3518..4105)
                     /locus_tag="VGM46_05100"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HEY4191999.1"
                     /translation="MPIIPKILVLAGSVRAGAYSGKTADVAQKELAVQGAEVTRISLA
                     DYPLPIIGEGDDAIPENATMLARQIAAHDGILLVTPEYNASLPPLLKNAIDWVSRVRL
                     HAGQPSSPLAGRAAALCSASTGPCGGIRAIGHLRAVLVHCRVEVVSPECSIGLAAQAF
                     DEAGELKDAKLSRAMQTVAQTLIERARMLSERIEP"
     gene            complement(4204..4809)
                     /locus_tag="VGM46_05105"
     CDS             complement(4204..4809)
                     /locus_tag="VGM46_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015314666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY4192000.1"
                     /translation="MMRGSELRKAFADKFDDELKFFKGWMDKPKAVGSIVPTSGVTSR
                     RMASVVDTASGLPVLEVGPGTGVITKAILARGVKPENLYAVEYSADFVRHLRRLYPEV
                     NVIEGDAFNLAETLGDKRDIMFDCAISAVPLLNFPVQRRIAYLEGLLARIPAGRPVLQ
                     ITYGPLSPIPAGKGDYTVKHYDFIIRNIPPAHLWVYRRVAH"
     gene            complement(4912..6039)
                     /gene="dnaJ"
                     /locus_tag="VGM46_05110"
     CDS             complement(4912..6039)
                     /gene="dnaJ"
                     /locus_tag="VGM46_05110"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEY4192001.1"
                     /translation="MKADFYETLGVQKGADEKELKSAFRKLAMQFHPDRNPGDHACEH
                     KFKEINEAYETLKDPQKRAAYDRFGHAAFEQGGMNGGGQGFGGGGFADIFEDIFGDMM
                     GGRRRSSGGRERGADLRYNMEISLEEAFAGKTAQIRVPASIACEECSGTGAKPGTQPV
                     TCGMCNGHGKVRASQGFFSVERTCPQCQGRGQTINDPCPKCDGQGRVVEERSLSVNIP
                     AGIEDGTRIRLANEGEAGQRGGPSGDLYIFLAVKPHEFFQRDGADLYCKVPISMTTAA
                     LGGQFEVATLDGTQTRVKVPEGTQNGRQFRLKGKGMPVLRQPNIGDLYIQTAVETPQN
                     LSRRQRELLEEFEQLSSKENSPQSSGFFSRMKDFFESFGEG"
     gene            complement(6188..7285)
                     /locus_tag="VGM46_05115"
     CDS             complement(6188..7285)
                     /locus_tag="VGM46_05115"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEY4192002.1"
                     /translation="MLFFRTLGLWRVAAAMLVMIFHFLIYGSTSGVQAAHRLRSLMPL
                     LDMFLMVSGFLIMFRYADRLMVERGSYLHFITRRLARFYPLYLATLVYFVFVGIAVHM
                     GLIGSSAAGRYDFATLPANILLVQGWGFTDTLTFNYVGWTLSAEWFCYLTLPVIVLTH
                     RRYGKGGLVALIAATLLALELGVAAGVIPFTSWQFADTWGAYRAFADFSIGALVAVLV
                     RDSTWGLRSPLPGWALFAVTVALMWQGLGGYPALALLALSIYLAALAERNDPQAMQRF
                     KPLDPIANASFSIYLIHPVIASLMLGIVWRRLLEPAHIVGFYVYWLVPMAVVVALALV
                     SARYFETPLGKLANARLAELFAARRKLSSAA"
     gene            complement(7494..>8097)
                     /locus_tag="VGM46_05120"
     CDS             complement(7494..>8097)
                     /locus_tag="VGM46_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009450925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEY4192003.1"
                     /translation="QGEREMAQDNKALGQFDLVGIPPAPRGVPQIEVTFDIDANGIVN
                     VSAKDKGTGKEHQIRIQASGGLSDADIEKMVKDAEANAENDKQRRALVEARNQAEALA
                     HSSEKSLKEYGEKVSDDDRKAISDAIAALKTAAEGEDAAEIEAKSQSLAEVSMKLGQA
                     MYEASQKEAAEADAKADAAKDDDVVDADFEEIDDDDKKPA"
BASE COUNT         1517 a         2544 c         2599 g         1437 t
ORIGIN      
        1 cgccaatctg tggccgccgg caatcgccat cctgcgccgc gaaacccatg gcaattatcc
       61 ggccgcgacc gccatcctga aatgcgtcta tgagggcctg ctggtgccgt tcgacacggc
      121 gcttcgcatc gagcagcgct acttcaccga gatcatgcag acgaaagaag cggcggcgat
      181 gatccgatcg ctgttcatct cgctacagga actgaacaag ggcgcgcggc ggccggacgg
      241 cgtgccggaa acgaagttca agaaaatcgg cgttctgggc gccggcttca tgggtgccgg
      301 catcgcctat gtcaccgcca aggccggcat tccggtggtg ctgctcgacc gcgacatggc
      361 ggcggccgaa aagggcaagg cgcattcgga aggcttgatg gacggcctga tcaaaaaggg
      421 ccgcgccacc cagcaggaca aagaaaagct gctggcgctg atcacgccga cggccgacta
      481 cgccaggctt gccggctgcg acctcgtcgt cgaggcggtg ttcgaggatt cgggcgtcaa
      541 gaaggccgcc accgaacagg cggaagccgt tttgaaaccc tcggcgatct ttgcctccaa
      601 cacctcgacc atcccgatca cggtgctggc gaagaacgcg gcgcggccga agaatttcgt
      661 cggcatccat ttcttctcgc cggtcgacaa gatgatgctg gtcgaaatca ttctcggcaa
      721 gaaaaccggc gacaaggcgc tggcgacggc gatcgatttc gtgcgcgcca tcaagaagac
      781 gccgatcgtc gtcaacgata cgcgcggctt ctacgtcaat cgctgcgtgc tgcgctacat
      841 ggcggaagcc tacaagatgc tgatcgaggg cgtgccggcc gcgatgatcg aaaacgccgc
      901 gcgcgccgcc ggcatgccgg tcggcccgct ggcgctgacc gatgaaacgg cggtcgatct
      961 ggcgcagaag atcatgcacc agacggtgcg cgacctcggc gacaaggcct tagatccaca
     1021 gcagatgaag ctgatcgata cgctggtcga caagcatcag cgccttggcc gcaagaacgg
     1081 caagggtttc tacgattatc cgcaaaaacc ggcgaagaag aagctatggc cggggctgag
     1141 ggacctctac ccgcagaaaa aggccgagga ggtcgatttc gaggagctga agcagcgcct
     1201 gctggcaacc atcgcgctgg aggcggcgcg ggtgatggag gaaaagatcg tcaccgaccc
     1261 gcgcgaggcc gatgtcggct cgatcctcgc cttcggcttc gcaccctata cgggcggcgc
     1321 gctgtcctac atcgacggca tcggcgccaa gaaattcgtg gcgctggcca agggattgca
     1381 gaagaaatac ggcgcggaat tcaagccgcc gaagcttttg atcgacatgg ccaacaaggg
     1441 tgaaaccttc taccagcgct tcgatcccta tgcgaaaggc gagacgaaga aggcggctta
     1501 gcaaaccggg aggagtaatt gggaggagcg ccgggcatga tcttcgacac gcatctgcac
     1561 ctgatcgacc ggtctgcctt gcattatccg tggctctccg gcgcgccggc gctgaaccgc
     1621 gatttctcat atgaagaata ttcggtgcag gcgcggcgcg ccggcatcga agcggcattg
     1681 cacatggagg tcgatgtcga tcccgccgac atcgaggccg agaccggcca tgtgaaggcg
     1741 ctggcggcgc gcgaaggcag cctgctgaaa ggcgcgatcg cttcctgccg accggaagac
     1801 agcggctttg ccgcctatct ggagcggcag ctggccgatc ccttcgtcaa aggttttcgc
     1861 cgggtgctgc atgtcgtgcc cgacgatatt tcgcagggcg cgctgtttcg cgaaaatatc
     1921 gggcgcatgg ccggctcgcg cctgactttc gatctcgtcg tgcagccgca ccagatcccc
     1981 aaggccatcg cgctggccga cctcgcgccg gatgtgcact tcgtgctcga ccattgcggc
     2041 gcacccgaca tcaagggcga cggcgagcat ccgtggcgcg agcatatggc ggagatcgcc
     2101 cggcggccaa acgtcaccgc caagatttca ggcgtcgtcg cctatgccga cgcacagagc
     2161 tggtcggtcg aaacgctcag gccctatgtc gagcatacga tttcggcttt cggctgggac
     2221 cgcgtggtgt ggggcagcga ctggccggtg tgcacgctgg gcggcgggct ggctgtctgg
     2281 gtggccgcca cgcatgcgct gctggccggg gtcggcatgg ccgaccgggc caagcttttg
     2341 tcgggcaatg cgcgccggct ctggagcctg tagggctacc gcgccgatat tcgctcaagc
     2401 gccttcgagc agcacgatct cgccatccgc cactgtcagt cggatcgcct tggcggcctg
     2461 atattcgggc gaatcgtagc attgtctcgc aatggcgagc gattcgaact cgatgatgac
     2521 gttgcgggcg cggcccggcc cctcgacatg gtccggctcg ccgccccgcg ccaggaattt
     2581 cgcgccgaag cgctcgaagg cgggcttcgc cgtcgagaca tagtccttgt agcgctccgc
     2641 gtcgcgcaca tcgacacgag cgatccaata tcccttgggc attctttttt tctccaggcg
     2701 cggcgcgcca tttcgattga acaccgtatt ccgaaaaccg gattacgctt ttcggcccga
     2761 tacccagagc ggttggccgc atttccggac gcaaaaccgt ctcacacttt gctggaaatg
     2821 ccctaggcgg cgcgcatctc gtcgagaata gcttgcgccg ccccccgcct gtcaaccgcg
     2881 ccgacgatgg gacggctgac gacgaggtgg ctggagccgt tgcggatggc ctgaccgggc
     2941 gtgaccacgc gcttctggtc gccgcggtcg gcccccgtgg ggcggatgcc cggcgtcacc
     3001 accgccatgt ccgggccgac gatacggcgc acggcctcgg cctcgcttgc cgagcagacg
     3061 atgccgccca tgccggcatg cagcgcctgc tcggcacgcc gcagcaccag cgcgcgcgga
     3121 tcgtattcgt agccggcgtc gatgacgtcc tgctcatcca tggaagtcaa aacggtgacc
     3181 gccagcaggc aaaggccgct gccctgcgcc gccttgaccg ccgcctgcat cgtctttgga
     3241 taggcatgca gggtcagcat ggtcatgccc atgcgcacga tgttttcgac gcccttggcg
     3301 accgtgttgt cgatgtcgag cagcttcatg tcgaggaaga ttttcttgcc gtcggccgcc
     3361 agatcgcggg cgaaatccag cccgccggca aacgcaagct ggtagcctat cttgtagaag
     3421 gaaacggcat cgccaagctc gcgcaccacg gtttcggcct ctgccacggt cggtacgtcg
     3481 agaccgacga tcagcctttc ccgcatggtt tcaacgttca tggctctatc ctttcagaaa
     3541 gcatgcgcgc gcgttcgatc aatgtttgtg ccaccgtctg catggcgcgg ctgagctttg
     3601 cgtccttcaa ttcgccggcc tcgtcgaagg cctgggcggc aaggccgatc gagcattcgg
     3661 gcgaaacgac ttccacccgg caatgcacca gcacggcgcg cagatggccg atggccctga
     3721 tgccgccgca aggcccggtg gacgccgaac acaaagccgc cgccctgccc gcgagcgggc
     3781 ttgatggctg gccggcatga aggcgcaccc ggctgaccca gtcgatggca tttttcagaa
     3841 gcggcggaag ggacgcatta tattcgggcg tgacgagcag aatcccgtca tgggcggcga
     3901 tctggcgcgc cagcatcgtg gcgttttcag gaatggcgtc gtcgccctcg ccgataatcg
     3961 gcagcggata gtcggccagc gaaatgcgcg tcacttccgc gccctgcacc gccagttcct
     4021 tctgcgccac gtcggctgtc ttgccgctat aggcgccggc gcggaccgag cctgcaagca
     4081 caaggatttt cgggatgatc ggcaagccgc gcgctccgtc tggagcatcg gacggaaagg
     4141 ggatgccgtt tcgatgctcg atcaaactgt tccggcgcag ctataggcga agcggcgccc
     4201 taatcaatgc gccacgcgcc ggtaaaccca aagatgggca ggcggaatgt tgcggatgat
     4261 gaagtcgtaa tgcttgaccg tatagtcgcc cttgccggca gggatcggcg atagcggccc
     4321 ataggtgatc tggagcaccg ggcggccggc gggaatgcgc gcgagcagcc cttcaaggta
     4381 ggcgatgcgg cgctggaccg ggaaattgag cagcggaacc gctgaaatcg cacagtcgaa
     4441 catgatgtcg cgcttgtcgc caagcgtttc ggcaagattg aaggcgtcgc cctcgatgac
     4501 gttgacctcc ggatagagcc ggcggaggtg gcgcacgaaa tcggcggaat attcgaccgc
     4561 atagagattt tccggcttca cgccgcgcgc caggatggcc ttggtgatga cgccggtgcc
     4621 cgggccaact tccagaacag gcaggccgga agccgtatcg acgaccgagg ccatgcgccg
     4681 cgacgttacc ccgctggtcg gcacgatcga gccgacggcc ttgggcttgt ccatccagcc
     4741 cttgaagaat ttaagctcgt cgtcgaactt gtcggcgaag gctttgcgca actcggaacc
     4801 acgcatcatg tctctctccc tggctccccg gatcgctgtt tagcagttac ggccccggag
     4861 acaagacggg caggcaaaag aatgtcgtgg acaagataaa ttctgtcgct ctcaaccttc
     4921 gccgaaagat tcgaagaagt ccttcatgcg cgagaagaag ccgctcgatt gcggtgaatt
     4981 ctccttggag gaaagctgct cgaactcctc cagcaactcg cgctggcggc gcgacaggtt
     5041 ctgcggcgtc tcgacggcgg tctggatgta aaggtcgccg atattgggct ggcgcagcac
     5101 cggcatgccc ttgcccttga ggcggaactg gcggccgttc tgggtgcctt cgggcacttt
     5161 cacgcgggtc tgggtgccgt cgagcgtcgc cacctcgaac tgtccgccaa gggcggcggt
     5221 ggtcatcgag atcggcacct tgcaatagag gtcggcgccg tcgcgctgga agaactcatg
     5281 cggcttgacg gcgaggaaaa tatagagatc gccggaaggg ccgccgcgct ggccggcctc
     5341 gccttcattg gcgaggcgga tacgggtgcc atcctcgatg ccggccggaa tgttgaccga
     5401 cagcgacctt tcctcgacca cgcggccctg gccgtcgcat ttcgggcacg ggtcgttgat
     5461 ggtctggccg cggccctggc actgcgggca ggtccgctcg accgagaaga agccctggct
     5521 ggcgcgcacc ttgccgtggc cgttgcacat gccgcaggtg accggctggg tgccgggctt
     5581 ggcaccggtg cccgaacatt cctcgcaggc gatggaggcc ggcacgcgga tttgcgcggt
     5641 cttgccggcg aacgcctctt ccagcgaaat ttccatgttg tagcgcaaat ccgcgccgcg
     5701 ctcgcgcccg ccggacgaac ggcgccggcc gcccatcatg tcgccgaaaa tatcctcgaa
     5761 aatatcggcg aagccgccgc cgccaaagcc ctggccgccg ccattcatgc cgccctgctc
     5821 gaaggcggcg tggccgaaac ggtcgtaggc ggcccgcttc tgcgggtcct tcagcgtctc
     5881 ataggcttcg ttgatttcct tgaacttgtg ttcgcaggcg tggtcgcccg gattacgatc
     5941 cgggtggaac tgcatggcga gcttgcggaa agcgctcttc agctccttct cgtcggcacc
     6001 cttctggacg cccagagttt cgtagaagtc agctttcatg tggtcctgct gttttcaatc
     6061 gcatcgggca agggtcaagt aaccgtgatg cccggcatgt gtcccgattt agtaagccgg
     6121 aggccgcaat gccatagttt ccagcccatg cggagatttt ttttcgcttt accgggggta
     6181 aatgcttcta ggccgcgctg gaaagctttc ttcgcgccgc aaaaagctcc gccagccggg
     6241 catttgccag cttgccgagc ggcgtttcga aatagcgggc cgacaccagc gctagcgcga
     6301 cgaccacggc catcggaacc agccagtaaa cgtagaagcc gacgatgtgc gccggctcca
     6361 gcagccggcg ccagacgatg cccagcatca gcgaagcgat gaccgggtgg atcaagtaga
     6421 tgctgaagga agcgttggcg atcgggtcga gcggcttgaa gcgctgcatc gcctgcggat
     6481 cgttgcgctc ggccaaggcg gccaggtaaa tcgacagcgc cagcagcgcc agcgccggat
     6541 agccgcccag cccctgccac atcagcgcaa ccgttacggc aaacagcgcc cagcccggca
     6601 gggggctgcg cagcccccat gtgctgtcgc gcacgagcac ggcaaccagc gcgccgatgg
     6661 aaaagtctgc aaaggcgcgg taggcgcccc atgtgtcggc gaactgccaa ctggtgaagg
     6721 ggatcacgcc ggccgcgacg cccagttcca gcgcaaggag cgtggcggcg atcagcgcca
     6781 ccagtccgcc cttgccgtag cggcggtgcg taagcacgat gaccggcagc gtcagatagc
     6841 agaaccattc cgccgacagc gtccagccga catagttgaa ggtcagcgta tcggtgaagc
     6901 cccagccctg gacgagcagg atgttggccg gcagcgtggc gaaatcatag cggccagccg
     6961 cgctggagcc gatcaggccc atgtgaacgg caatgccgac aaagacgaaa tagacgaggg
     7021 tcgccagata gagcgggtaa aagcgcgcca gccggcgggt gatgaaatgc agatagctgc
     7081 cgcgctcgac catcagcctg tcggcataac ggaacatgat gaggaagccg gacaccatca
     7141 ggaacatgtc cagcagcggc atgaggctgc gcagcctgtg ggctgcctga acgccgctgg
     7201 tcgagccgta gatgaggaaa tggaagatca tcaccagcat cgcggcggcg acacgccaga
     7261 gcccgagtgt ccgaaaaaac agcatatgcg gccagcccct ccgtccgaaa catcgaaacg
     7321 cgcgggcgca ggattagcac cggaaaacgc cgctgtgccg gaaaggagtt gttaagctta
     7381 acgccggcgg ccgctgtgga caaagtgggg cggacaagca aaaagcccgg cgcgaaggcc
     7441 gggctttttc attttttcga agcggcgatc aggccggctt ttcgaagcag cgcttaggcc
     7501 ggcttcttgt cgtcgtcgtc gatttcctcg aaatcggcgt cgaccacatc gtcgtccttg
     7561 gcggcatccg ccttggcgtc ggcctcggcg gcttccttct ggctggcctc gtacatcgcc
     7621 tgcccgagct tcatcgaaac ctcggccagc gactggctct tggcttcgat ctcggccgca
     7681 tcctcgcctt cggccgcagt cttcagcgcc gcgatggcat cggaaatcgc cttgcggtcg
     7741 tcgtcggata ccttctcgcc atattccttc agcgacttct ccgaagaatg cgccagagcc
     7801 tcggcctggt tgcgggcctc gaccagcgca cggcgctgct tgtcgttttc cgcattggcc
     7861 tcggcgtcct tgaccatctt ctcgatgtcg gcgtcggaaa gaccgccaga cgcctggatg
     7921 cggatctggt gttccttgcc ggtgcccttg tccttcgccg agacgttgac gatgccgttg
     7981 gcgtcgatgt cgaaggtgac ctcgatctgc ggcacgccgc gcggcgccgg cgggatgccg
     8041 accaggtcga actggccgag cgccttgttg tcctgcgcca tttcacgctc gccctgg
//
LOCUS       DASYCS010000158         4612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2644063,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000158 DASYCS010000000
VERSION     DASYCS010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4612
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2644063"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="VGM46_05125"
     CDS             complement(<1..744)
                     /locus_tag="VGM46_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019856979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEY4192004.1"
                     /translation="MNSPQPASQRQRLDELLVGRGLFASRSRARDAIERGAVTVDGVV
                     VRKAGHGAAHDAAIAIDDPAQAYVSRAALKLIAGLDHFGFDPGGSEALDIGASTGGFT
                     QVLLERNAAHVTAIDVGHGQIHAVIAADPRVTVVQGLNARELTLADLAGRTPDFLACD
                     VSFISLKLALPPALALAAAGAKAILLVKPQFEAGREAIGKGGLLKNPDDAGRMAQELR
                     QWLDAVPGWRALGVHPSPIEGGDGNREFLL"
     gene            complement(803..1090)
                     /locus_tag="VGM46_05130"
     CDS             complement(803..1090)
                     /locus_tag="VGM46_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192005.1"
                     /translation="MNKMLGAAAMLAAFAVPSSAGTRYDHGLEKAAMDIVAGKMGDIR
                     GGFSYAQKPQMVVSPGKTPAPNASPPLMPTAGDSVEALPLQSGPAPSITTF"
     gene            complement(1235..3148)
                     /gene="dxs"
                     /locus_tag="VGM46_05135"
     CDS             complement(1235..3148)
                     /gene="dxs"
                     /locus_tag="VGM46_05135"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006330582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEY4192006.1"
                     /translation="MNSKPATPLLDTVRLPADLRTLGENQLPQLAQELRAELVDAVSE
                     TGGHLGAGLGVVELTLALHYVFNTPDDRIIWDVGHQAYPHKILTGRRDRIRTLRQEGG
                     LSGFTKRDESEYDPFGAAHSSTSISAGLGMAVARDLSGGVNNVIAVIGDGALSAGMAY
                     EALNNAGALDARLIVILNDNDMSIAPPTGAMSAYLARLASGKTYLGIRDFGKKLTSYL
                     GKRADRAIKRAVEHARGYVTGGTLFEEMGFFHIGPIDGHNLEHLIPVLRNVRDHGNGP
                     VLIHVVTQKGKGYAPAEAAPDKYHGVNKFDVITGAQAKAPANAPSYTKVFGESLVQEA
                     REDDRIVAITAAMPAGTGVDLFGKEFPSRTFDVGIAEQHGVTFAAGLATEGYRPFAAI
                     YSTFLQRAYDQVVHDVAIQGLPVRFPIDRAGYVGADGPTHCGAFDTTFLATLPGFVVM
                     APSDEAELKHMVRTAAAYDSGPISFRYPRGNGVGVDMPERGSILEIGKGRIVREGTKV
                     ALLSFGTRLHDCLLAAEELGAAGLSTTVADARFAKPLDEEMIRRLARSHEVLVTVEEG
                     AVGGFASHVLQFLAKEGLLDSGLKVRPLVLPDVFTDHAKPEKMLADAGLDAAGIVRAV
                     FMALGRATHAQRA"
     gene            complement(3232..4152)
                     /locus_tag="VGM46_05140"
     CDS             complement(3232..4152)
                     /locus_tag="VGM46_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEY4192007.1"
                     /translation="MSFFPGNDPNVGDASACDQIELMVIPNARDIGGFQVRRALPSAK
                     RRMVGPFIFFDRMGPALLRAGQALDVRPHPHIGLSTVTYLFDGKIRHRDSLGTEMVIE
                     PGDVNLMTAGRGIVHSERMPEELRGTPMSVSGLQTWLALPDDKEEIAPVFTNTGKALL
                     PQFDADGVKGRLIIGEFSGLRSPVKPTSETLYADIRLTPGARVEIPADAEERAIYTLE
                     GEVAISGDVFPAERLLVFKPGDEIVVSSQSGAHFMLFGGASLASKRYIWWNFVSSSRE
                     RIEQAKEEWKTGRFDIVPGDEEEFIPLPEA"
     gene            complement(4156..4545)
                     /locus_tag="VGM46_05145"
     CDS             complement(4156..4545)
                     /locus_tag="VGM46_05145"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY4192008.1"
                     /translation="MRILLDTHVAIWALTQPRLIPDRIGSVIKEEETSVFVSVAVIWE
                     IAIKSSLNRASAPDVDAARIVQMCNDAAYEIVPVTPAHAVAVASLPHIHADPFDRIMI
                     AQAIIEPMRLVTHDKVVAAYDKNFLSW"
BASE COUNT          892 a         1429 c         1473 g          818 t
ORIGIN      
        1 aagcaggaat tcgcggttgc cgtcgccgcc ttcaatgggt gatggatgaa caccgagcgc
       61 gcgccagccg ggcacggcgt cgagccattg ccgcaattcc tgcgccatgc gcccggcatc
      121 gtcgggattt ttcagcaggc cgcccttgcc gatggcctcg cgcccggctt cgaactgcgg
      181 cttgaccagc aagatcgcct tcgcgcctgc ggcggccagg gcgagagccg gcggaagcgc
      241 cagcttcaac gagatgaagc tgacatcgca ggcgagaaaa tcgggcgtcc ggccggcgag
      301 gtcggcaagc gtcagttcgc gcgcgttgag gccctggaca accgtcacgc gcggatcggc
      361 ggcgatgacg gcgtggatct ggccatgacc gacatctatt gcggtgacat gggcggcgtt
      421 gcgctccagc agcacctgcg tgaaaccgcc ggtcgacgcg ccgatatcga gcgcctcgct
      481 cccgccgggg tcgaacccga aatggtcaag cccggcaatc agcttcagcg ccgcgcgcga
      541 gacataggcc tgcgccggat cgtcgatggc aatggcggca tcgtgtgccg cgccatgacc
      601 cgccttgcgc acgaccacac cgtcaacggt aacggcgccg cgttcgatgg cgtcgcgggc
      661 gcgcgaacgg ctggcaaaca gcccgcgccc gacgagcagt tcgtcgagcc gctggcgctg
      721 gctggctggc tggggcgaat tcatcggcct tcattggcga atgcgcggcg tgaaggcaag
      781 cccgccgctg cctgccgcga gatcagaagg tggttatgga aggcgccgga cccgactgta
      841 acggcaaggc ttcaacgcta tcgccggcgg tcggcatcag cggcgggctc gcgttcggcg
      901 cgggcgtctt tccggggctg acgaccatct gcggcttttg ggcataagag aagccgccac
      961 ggatatcgcc catcttgccg gcaactatat ccatcgccgc tttttcgagg ccatggtcgt
     1021 agcgcgtccc ggccgacgac ggcacggcga aggcggcaag catggccgcc gcgcccagca
     1081 tcttgttcat tcttcaagct cccttgggaa aatccctagc aggctcgctt gaaaaaaccg
     1141 ccaagagaga cggtaagaaa gcatcaaaag acaggctgaa caacaggtta cggcaacccg
     1201 cccatgaagt gattcaggcc gttgataatt tgaatcaggc gcgctgggcg tgagtggcgc
     1261 ggccgagggc catgaatacc gcgcgcacga tgccggcggc atccagcccg gcatcggcaa
     1321 gcatcttttc aggcttggca tggtcggtga aaacgtccgg caacaccaat ggccgcactt
     1381 tcaggccgct atccagcaag ccttccttcg ccaggaattg caggacgtgg ctggcaaatc
     1441 cgccgaccgc gccctcctcc accgtcacca acacttcatg cgaacgggca aggcggcgga
     1501 tcatctcctc gtccagcggc ttggcgaaac gcgcgtcggc aacggtggtc gaaaggccgg
     1561 cggcgcccag ttcctcggcg gcgagcaggc aatcgtgcaa gcgcgtgccg aacgatagaa
     1621 gcgccacctt cgtgccttca cgaacgatgc gccccttgcc gatttcaagg atcgaacccc
     1681 gctccggcat gtcgacgccg acaccgttgc cgcgcggata acgaaacgag atcgggccgc
     1741 tgtcataggc ggcagcggtg cgcaccatgt gcttgagttc ggcctcgtcg cttggcgcca
     1801 tgaccacgaa gcccggcagc gtcgccagga acgtggtgtc gaaggcgccg caatgggtcg
     1861 ggccatccgc gccgacatag ccggcgcggt cgatcgggaa gcgcaccggc aggccctgga
     1921 tggccacgtc gtgcacgacc tgatcgtaag cgcgctggag aaaggtggaa tagatggcgg
     1981 cgaatggccg gtagccctcc gtggcaagcc ccgccgcaaa cgtgacgcca tgctgctcgg
     2041 caatgccgac atcgaaggtg cgcgaaggaa actccttgcc gaacagatcg acgccggtgc
     2101 cggccggcat ggcggcggtg atcgccacga tgcgatcgtc ctcacgggct tcctggacga
     2161 ggctctcgcc gaacaccttg gtatagcttg gcgcgttggc cggagccttg gcctgtgcgc
     2221 cggtgatgac gtcgaacttg ttgacgccgt gatatttatc gggcgcggcc tccgccgggg
     2281 cgtagccctt gcccttctgg gtcacgacat ggatcagcac cgggccgttg ccgtggtcgc
     2341 ggacgttcct cagcaccggg ataagatgct ccagattgtg accgtcgatc gggccgatgt
     2401 ggaagaaacc catttcctca aacagcgtac cgccggtgac atagccgcgc gcgtgttcca
     2461 cggcccgctt gatggcgcgg tcggcgcgct tgccgagata ggaggtcagc ttcttgccga
     2521 aatcgcgaat gccgagatag gtttttcccg aggcaaggcg tgccagataa gcgctcatcg
     2581 cgccggtcgg cggcgcaatc gacatgtcgt tgtcgttgag gatgacgatc agccgggcat
     2641 ccagcgcgcc ggcattgttc agcgcttcat aggccatgcc ggccgacagc gcgccgtcgc
     2701 cgatgacggc gatgacattg ttgacgccac ccgaaaggtc gcgggccacc gccatgccga
     2761 gaccggcgga aatggaggtc gacgagtgcg ccgcgccgaa cggatcatat tcgctttcgt
     2821 cgcgcttggt gaagccggaa agcccgcctt cctggcgcag cgtgcggatg cggtcgcgcc
     2881 ggcccgtcaa tatcttatgc ggataggcct gatggccgac atcccagatg atgcggtcgt
     2941 ccggagtatt gaatacatag tgcagggcaa gggtcagttc gacgacgcca agccccgcgc
     3001 ccagatggcc gccggtttca gagacggcat cgacgagttc cgcgcgaagc tcttgcgcca
     3061 gttgcggcag ctgattctcg ccaagggttc gcaaatccgc cggaaggcga accgtgtcga
     3121 gaaggggcgt ggcgggtttc gaattcaatt tatgcgggcc tgcggactta tctgttcgca
     3181 tccgaagtag gatgccaacg gtggaatgta aatgtgcgcc ggtgccgcgc gtcatgcctc
     3241 gggcagcgga ataaactctt cctcgtctcc cggcacgata tcgaagcggc cggtcttcca
     3301 ctcttccttg gcctgctcga tacgctccct ggaagaggaa acgaagttcc accagatgta
     3361 gcgcttcgag gcaagcgatg cacccccgaa cagcatgaaa tgcgcaccgg attgcgacga
     3421 aacgacgatc tcgtcgcccg gtttgaagac caaaagccgc tcggcgggga aaacatcgcc
     3481 ggaaatggcc acctcccctt cgagcgtata gatggcgcgt tcctcggcat cggccgggat
     3541 ttcgacgcgc gcgccgggcg tcaggcggat gtcggcgtaa agcgtttccg aggtgggctt
     3601 gaccggggag cgcaagccgg aaaactcgcc gatgatgagg cggcccttaa cgccgtcggc
     3661 atcgaattgc ggcaacaagg ccttgcccgt attggtgaag acgggcgcta tttcttcctt
     3721 gtcgtcaggc agcgccagcc acgtctgcaa gccggaaacc gacatcggcg tgccgcgcag
     3781 ctcctccggc atgcgttcgg aatgaacgat gccgcgtccc gccgtcatca ggttcacgtc
     3841 gcccggctcg atcaccattt cggtgccaag cgaatcgcgg tgcctgatct tgccgtcgaa
     3901 cagataggtg acggtggaca ggccgatatg cggatgcggg cgcacgtcga gcgcctgccc
     3961 tgccctaagc agcgccggcc ccatgcggtc gaagaagatg aacggcccga ccatccgccg
     4021 ctttgccgaa ggcagcgcgc gccgcacctg gaagccacca atgtcgcgcg cattgggtat
     4081 caccatcagc tcgatctggt cgcaggccga ggcgtcgccg acattgggat cgttaccggg
     4141 aaagaagctc atgagtcacc agcttaggaa attcttatcg taggcggcta ccactttatc
     4201 atgcgtaaca agccgcatgg gttcaattat ggcctgtgcg atcatgatcc tgtcgaacgg
     4261 atcggcatgg atgtgcggaa gtgatgctac tgccactgca tgggctggtg tgaccggcac
     4321 gatttcgtag gcggcatcat tgcacatctg tacaattcta gccgcatcga catctggcgc
     4381 cgatgctcga ttaagcgaag atttaatggc gatttcccaa atgacggcaa ccgagacgaa
     4441 aacggatgtt tcctcttcct tgataaccga gccaatgcga tccggtataa gccttggctg
     4501 ggtcaatgcc caaatggcca catgggtgtc gagcagtatc ctcaaggttt atccacgccg
     4561 aacatctcgg caatttcggc gtccgtcgaa ttccattctt caagcgtaaa cg
//
LOCUS       DASYCS010000159         4831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4385184,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000159 DASYCS010000000
VERSION     DASYCS010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4831
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4385184"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /gene="rfaD"
                     /locus_tag="VGM46_05150"
     CDS             <1..723
                     /gene="rfaD"
                     /locus_tag="VGM46_05150"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HEY4192009.1"
                     /translation="ACSTTTEWNGKFMMEVNYAYSKRLLHACLGLRVPFLYASSASVY
                     GGGGDFREEPECEHPLNVYAYSKKLFDDYVRRNVFGTEHSPVAGLRYFNVYGPREQHK
                     GAMASVAFHLFNQVGRGENPKLFGAYDGFGPGEQSRDFIHVGDVADVNLWLWKKGASG
                     IFNCGTGRAQPFRAIAETVIDTLGKGEIEFIAFPDHLKGSYQSFTQADMSRLRAAGYN
                     GQFRSVEAGVREYVEWLQAPRS"
     gene            699..1697
                     /gene="waaF"
                     /locus_tag="VGM46_05155"
     CDS             699..1697
                     /gene="waaF"
                     /locus_tag="VGM46_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEY4192010.1"
                     /translation="MAASPAILVIGPRWVGDMVMAQCLLAALKQLHPQAAMDVVAPAW
                     AAPLLARMPQVRQRIEATTKPGSLGLAERWRFGRSLRGRYDAAYVLPGSWKSALIPFF
                     AGIRQRVGHKREMRYGLLTDIVPLPKDLKRRTAQAYFSLARGGNFQPPRLTVDAENQA
                     SLLSRHGLGVKQFVAMMPGAEFGPAKRWPSAHYAGLARAMMAKGLGVVLLGSRNDAGV
                     TAEIAALAPGCIDLAGQTRLEDAIDLIAAARLAVANDSGLMHVAAAVETPIVAIYGST
                     SPNNTPPLAEQRELVWLGLSCSPCHQKICPLGHLNCLNTLDVSLVVSAADRLLEAA"
     gene            1697..2656
                     /gene="waaC"
                     /locus_tag="VGM46_05160"
     CDS             1697..2656
                     /gene="waaC"
                     /locus_tag="VGM46_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013530067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HEY4192011.1"
                     /translation="MKVLIVKTSSMGDVIHTFPAIEDARRARPDIAFDWCVEEAFAPI
                     VALHPAIRKIHTVAIRRWRKQLLDRETRREACALRCAVRDERYDLVIDAQGLLKSAIV
                     ARLAGAPIAGFDRASAREPSATLFYNQRYAVARNLHAIERTRRLLGLALGYRPDFSTL
                     DSGIAPPAVPDAKTAFLLHGTSREDKKWPVEDWIETARLLVVARITPVTTWSNEREKT
                     VAQAIAAAVPRTVLVKKSPLAEVATLLGQSSLVIGADTGLTHLANAYGLPTVAIFLTT
                     QPGLTGPIGPFSSTLLAEKRAPVTPAQVMAEAKRLLGLHSSNT"
     gene            complement(2805..3278)
                     /gene="greA"
                     /locus_tag="VGM46_05165"
     CDS             complement(2805..3278)
                     /gene="greA"
                     /locus_tag="VGM46_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEY4192012.1"
                     /translation="MNKVPMTGEGFASLKEELRWRQQEERPRIIEAISEARSHGDLSE
                     NAEYHAAKEQQSLNEGRVGELEDFIARAEVIDITKLSGDKIKFGATVMLVDEDTEEKK
                     TYKIVGDQEADVKSGRISISSPIARALIGKEVGETIEVNAPGGPRGYEIVKVRFI"
     gene            complement(3457..3807)
                     /locus_tag="VGM46_05170"
     CDS             complement(3457..3807)
                     /locus_tag="VGM46_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004688588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HEY4192013.1"
                     /translation="MAVLLSPRVLPILMLIASNVFMTFAWYGHLKFKSSPIYLAVIVS
                     WMIAFFEYWLAVPANRIGSGVYSAAELKTMQEVITLSVFAVFSVFYLKEALTWNHAFG
                     FALIAGGAALIFKG"
     gene            3952..4377
                     /locus_tag="VGM46_05175"
     CDS             3952..4377
                     /locus_tag="VGM46_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3775 domain-containing protein"
                     /protein_id="HEY4192014.1"
                     /translation="MQQRLETEWELSIGPDTVRLFILKAKGLAAAVNEDYADGAEHEV
                     ELDGNSRDKHHHDGLAEEKSENLAEEELRELINDLNVDESAELVALAWVGRGDYVASE
                     WADALAAARERPYRRTAKYLLGLPQLADWLEEGLETIGA"
     gene            4546..>4831
                     /locus_tag="VGM46_05180"
     CDS             4546..>4831
                     /locus_tag="VGM46_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192015.1"
                     /translation="MGQAATMRHHLPLPDMVSPRLRLTVFLAILFLACPMGLIAATAK
                     PPKLPRRAPVPQLQERPPGVVPMPAAKPGEADFPKPKEAVKPNAAETTLPQ"
BASE COUNT          941 a         1571 c         1452 g          867 t
ORIGIN      
        1 gcgtgttcca ccaccaccga gtggaacggc aagttcatga tggaggtgaa ctacgcctat
       61 tcgaagcgcc tgctgcacgc ctgcctcggt ctgcgcgtgc cgttcctcta cgcctcctcg
      121 gcctcggtct atggcggcgg cggcgatttc cgcgaagagc cggaatgcga gcatccgctc
      181 aacgtctacg cctattccaa gaagctgttc gacgattatg tccgccgcaa cgtgtttggc
      241 accgagcatt cgccggtggc gggcctgcgt tacttcaacg tctacggccc gcgcgagcag
      301 cacaagggcg cgatggcatc ggtcgccttc cacctgttca accaggtcgg acgtggtgag
      361 aaccctaaac tgttcggagc ctatgacggc ttcggaccgg gtgagcagag ccgcgatttc
      421 atccacgtcg gcgatgtggc ggatgtgaat ttatggcttt ggaaaaaagg cgccagcggc
      481 atcttcaact gcggcactgg ccgcgcccag ccattccgtg ccatcgccga aacggtgatc
      541 gacacgttgg gcaagggcga gatcgagttc atcgccttcc ccgaccacct caagggcagc
      601 taccagagct tcacccaggc cgacatgtcg cggctgcgcg ccgccggcta taatggccag
      661 ttccgctcgg tggaagccgg cgtccgggaa tatgtcgaat ggctgcaagc cccgcgatcc
      721 tagtcatcgg cccgcgctgg gtcggcgaca tggtgatggc gcaatgcctg ctggcagcgc
      781 tgaaacagct gcacccgcag gccgccatgg acgtggttgc gcccgcatgg gcggccccgc
      841 tgctggcgcg catgccgcaa gtgcgccagc gcatcgaagc gacaaccaag cccggcagcc
      901 tgggcctggc cgaacgctgg cgtttcggcc gctcgctgcg cggccgttat gacgcagcct
      961 atgtcctgcc cggcagctgg aaatcggcgc tgatcccgtt tttcgccggc atccgccagc
     1021 gcgtcggcca caagcgcgag atgcgttacg gcctgctcac cgatatcgtg ccgctgccca
     1081 aagacctcaa gcggcgcacc gcgcaagcct atttctcgct ggcgcgcggc ggaaattttc
     1141 agccgccccg attgactgtc gatgccgaaa accaggcgtc cctgctttcg cgccatggac
     1201 ttggcgtgaa acaattcgtg gcgatgatgc ccggcgccga gttcggcccg gccaagcgct
     1261 ggccaagtgc gcactatgcc ggccttgccc gcgccatgat ggccaaaggg cttggcgtgg
     1321 tgttgctggg ttccaggaac gacgccggcg ttaccgctga aattgccgcc cttgcgccgg
     1381 gttgcatcga cctcgccggg cagacaaggc tggaagacgc catcgacctg atcgccgcgg
     1441 ccaggctcgc tgtcgccaac gatagcgggc tgatgcatgt cgcggcagcc gtcgaaaccc
     1501 ccatcgtcgc catctatggc tcgacctcgc cgaacaacac gccgccactg gcagagcagc
     1561 gcgaactggt ctggctcggc ctttcctgtt cgccctgcca ccagaagata tgtccgcttg
     1621 gccacctcaa ctgcctgaat acgctggatg tctcgctggt cgtctcggcg gctgaccggc
     1681 tgctggaagc agcctgatga aggtcctgat cgtcaaaacc tcgtcgatgg gcgatgtcat
     1741 ccataccttt ccggcgatcg aggatgcccg ccgcgcccgg cccgacatcg ccttcgactg
     1801 gtgcgtggag gaagccttcg ccccaatcgt cgcgctgcat ccggccatcc gcaaaatcca
     1861 caccgtcgcc atccgccgct ggcgcaagca actgctggac cgcgaaaccc ggcgcgaggc
     1921 gtgcgcgctg cgctgcgccg tgcgcgatga gcgctacgac ctcgtcatcg acgcgcaagg
     1981 actgttgaaa tcggccatcg tggcaaggtt ggccggtgcg ccgattgccg gcttcgaccg
     2041 cgcaagcgcg cgcgagcctt cagccacgct gttctataac cagcggtacg cggtcgcccg
     2101 caatctccac gccatcgagc gcacaaggcg gctgctcggc ctggcgcttg gctatcggcc
     2161 ggatttctcc acgctcgatt caggcatcgc gccgcctgcc gttccggatg cgaagacggc
     2221 tttcttgctg cacggcacca gccgcgagga caagaaatgg ccggtggagg attggatcga
     2281 aaccgcgcgc ctgctggtcg tcgcccgcat aactccggtc acgacatggt cgaacgagcg
     2341 cgaaaagacg gtggcgcagg ccatcgccgc tgccgtgccg cgaaccgttc tggtgaaaaa
     2401 atcgccgctg gctgaagtcg ccacccttct ggggcagtct tcgctggtga ttggggccga
     2461 caccggcctg acccatctgg ccaatgccta cggcctgcct accgtcgcca tattcctgac
     2521 cacgcagccc ggcctgaccg ggccgatcgg gccgttttca tcgacattgc tggcggaaaa
     2581 acgcgcgccc gtaacacccg cgcaggtgat ggccgaagcc aaaagactgc tcggccttca
     2641 cagttccaat acttgattca aagaatctac ctcaaaaaaa gcggcaggcc ggaccagaat
     2701 ggcggctgga gcaccgcagc gcggcatgct tcgcgacagg taagcactgg aagcgcacga
     2761 accgccatcc cacaaccggg catggcggtt gtcgacccgg atgctcagat gaaccgcacc
     2821 ttgacgatct catagccgcg cggaccgccg ggcgcattga cctcgatggt ttcgccgact
     2881 tccttgccga tcagcgcgcg cgcgatgggt gaagaaatgg agatgcggcc cgacttcacg
     2941 tccgcttcct ggtcaccgac gatcttgtaa gtcttctttt cctcggtgtc ctcgtcgacc
     3001 agcatgacgg tcgccccgaa cttgatcttg tcgccgctga gcttggtgat gtcgatcacc
     3061 tcggcgcgcg cgataaaatc ctccagttcg ccgacgcgac cttcattgag actctgctgc
     3121 tccttggcgg catggtactc cgcattttcg gaaaggtcgc catgcgagcg cgcctcggag
     3181 atcgcctcga tgatgcgcgg cctctcctcc tgctggcgcc agcggagttc ttccttcagc
     3241 gaagcaaaac cctcgcctgt catcgggacc ttgttcatgg cctgaccctt ctgctctgcc
     3301 caccctgtct cgggtggggt cgtcgcacaa aaagaaaacg gtccgtcgga tcgaaccaac
     3361 gaaaccgtct cacctcggat tctgtgaata tagcgatctt ctcgcggttt tcacgcccaa
     3421 aatttgaagc cgaagaaata atgccgaggg agcgcctcaa cctttgaaaa taagcgccgc
     3481 tccgccggcg atcaacgcga aaccgaaggc gtggttccac gtcaaggctt ccttgaggta
     3541 aaacacggaa aatacggcga ataccgacag cgtgatgact tcctgcatcg tcttcagctc
     3601 ggcggcggaa tagacgccgc tgccgatgcg gtttgccgga accgccagcc aatattcgaa
     3661 aaacgcaatc atccagctga cgatgacggc cagatagatc ggcgaactct tgaatttgag
     3721 atgcccgtac cacgcaaacg tcatgaaaac gttggaggcg atgagcatga gaatgggcag
     3781 aaccctgggc gacaaaagca cagccatgcg gaaaccattc tggcgggatg cgggaacgcc
     3841 atttcatggc aaccgccctt gctgtcaatc gggcaaactt tggaccatcg aagaaagggt
     3901 gacgatcccg gcaaataggc tatcatcccc gcaacagcac ggagggagcc ggtgcagcag
     3961 cgtcttgaaa cggaatggga gctttcgata gggccggaca ccgtgcgcct gttcatcctg
     4021 aaggccaagg gactggccgc cgccgtcaac gaggactatg ccgacggcgc cgagcacgag
     4081 gtcgagcttg acggcaacag ccgcgacaag caccaccatg acgggctggc cgaggaaaaa
     4141 tccgaaaacc tggcagaaga agagctgcgc gaactgataa acgacttgaa cgtcgatgag
     4201 agcgccgagc tggtcgcgct ggcatgggtc ggccgcggcg actacgtcgc ttccgaatgg
     4261 gccgatgcgc tggccgccgc ccgcgagcgt ccgtaccggc gcaccgcgaa atatctgctc
     4321 ggccttccgc agcttgccga ctggctggaa gaggggttgg aaacgatcgg cgcctgaggc
     4381 cctcgacggt tcgttcgagc ctaaggcagg ccgcaacgcc tgaggcagat aatcctttca
     4441 acgtaacagc ttgaggcgaa ctcccgaatc ggtatgtcag cgcgctatcg ccactgattt
     4501 tatttgcgcc ggccggcctc cgcgacaaaa cggtcaccga atgtgatggg ccaggccgca
     4561 accatgcggc atcatttgcc gttgccggac atggtttcac cgcgccttcg tctaacagtc
     4621 ttcctcgcca ttctgttcct ggcctgtccg atgggtctga ttgccgcgac ggcaaaaccg
     4681 ccgaagcttc cgcgccgggc gccggtgccg caactgcaag aaaggccacc gggcgtcgtt
     4741 cccatgccgg cggcgaagcc tggcgaagcc gatttcccga agccaaagga ggctgtaaag
     4801 ccgaacgctg cggaaaccac gctcccgcaa c
//
LOCUS       DASYCS010000160         4114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_759838,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000160 DASYCS010000000
VERSION     DASYCS010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759838"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VGM46_05185"
     CDS             complement(<1..345)
                     /locus_tag="VGM46_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006325152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEY4192016.1"
                     /translation="MAAPKLVIFDCDGVLVDTETLANQRLAQWISEGGYPITYAQCRQ
                     RFVGRSLVSVHAELKTLGTDLGDDFVERWYRELPAIFGSGVEKIPYVETFIAAVEAAG
                     VAHCVASSARLEK"
     gene            complement(346..831)
                     /gene="dut"
                     /locus_tag="VGM46_05190"
     CDS             complement(346..831)
                     /gene="dut"
                     /locus_tag="VGM46_05190"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEY4192017.1"
                     /translation="MSATPQGTSVIGPAVGFIRLPHGAGLPLPVYESAGAAGMDLRAA
                     VEDDRPMLILPGRRALVPTGLAVEIPQGMEGQVRPRSGLALKHGITCLNTPGTIDSDY
                     RGEVKVLLVNLGDEDFVVTRGMRIAQIVFAQVTQITAEERDAAGRTARGAGGFGSTGA
                     A"
     gene            1032..2621
                     /locus_tag="VGM46_05195"
     CDS             1032..2621
                     /locus_tag="VGM46_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008596288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEY4192018.1"
                     /translation="MTETIKDAVPRRRTFAIIAHPDAGKTTLTEKLLLFGGAIQLAGE
                     VKAKKDRIQTRSDWMKIERERGISVVTSVMTFEYEDNVFNLLDTPGHEDFADDTYRTL
                     SAVDSAVMVIDGAKGIEPRTLKLFEVCRLRNIPIVTFVNKMDREARDTFEILDEIEQK
                     LALDTAPITWPIGRGKNFAGTYHLAQNAVRRGDAERDRTPVNGPDSNRVAGLLPENER
                     EAFIEELDLAREACRPFDVDAFREGHMTPVYFGSALRNFGVRDLIEALGAYGPPPRAQ
                     EADTRTVEATEDKMTSFVFKIQANMDPNHRDRIAFVRVCSGRLERGMKAKLVRTGKPM
                     SLSAPQFFFARSRITADEAYAGDVVGIPNHGTLRIGDTLTEGEELLFRGVPNFAPEIL
                     RRVRLGDAMKAKKLKEALHQMAEEGVVQLFSPEDGSPAIVGVVGALQLDVLKERLNIE
                     YTLPVDFEMSRFSVCRWISADDKAEVHRFIEAHRGDIARDLDNDPVFLAQHAFSLNYE
                     AERWKAIRFTDVKDYQVRDAA"
     gene            complement(2654..>4114)
                     /locus_tag="VGM46_05200"
     CDS             complement(2654..>4114)
                     /locus_tag="VGM46_05200"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4192019.1"
                     /translation="DQFGRAKMYNLGFAIFTLASIALGLLPGQGDFAAVYLVSVRIVQ
                     GVGGALLMANSTALLTDAFPVEERGFALGINVVAAIGGQFLGLLIGGLLADTNWRLVF
                     WINVPFGLAGTIWAYWKLHDAPDRKKHTIDWIGNISFGLGLVLILTAITYGLQPYGQQ
                     TMAWTSPKVLTLFAIGILSLIAFVVVERRVARPMLDLRLFRIPAFAYGNIANLVSGVA
                     RGGLQFMLIVWLQGIWLPMHGYSFEQTPLWSAIFMLPLTVGFLIAGPASGYLSDRFGG
                     VPFAVGGMLAGAASFVALMALPADFSYTIFAALLFLNGLGSGLFVAPNSTQIMNSVPA
                     AERGQASGMRATTTNVGQVLSIGLFFSLMLLGLASSLPQAMEAGLLAQHVPADIAHQV
                     ANLPPVSSLFAAFLGYNPMGELIPANVLAALPAQSVATLTGNTFFPDLMAGPFKHGLV
                     FAFSFSAILYVIAAITSWLGGDRVAGAAPEMAEARG"
BASE COUNT          798 a         1294 c         1377 g          645 t
ORIGIN      
        1 cttttcgagg cgcgccgagg aagcgacgca atgcgcgaca ccggccgctt cgacggcggc
       61 gatgaacgtt tcgacatagg ggattttttc gacgccgctg ccgaagatgg cgggcagttc
      121 gcgataccag cgctcgacga aatcatcgcc tagatcggtg ccgagggttt tcagttccgc
      181 atgcacggag acaaggctac ggccgacgaa ccgctggcgg cattgcgcat aggtgatggg
      241 atagccgcct tcgcttatcc attgcgccag ccgctggttg gcgagcgtct cggtatcgac
      301 gagcacgccg tcgcaatcga agatgaccag ttttggcgca gccattcagg cggcgccggt
      361 cgagccgaag ccgcccgcgc cgcgcgccgt ccggccggcg gcatcgcgtt cctcggcggt
      421 tatctgcgtc acctgggcaa agacgatctg cgcgatgcgc atgccgcgcg tcaccacgaa
      481 atcctcgtcg cccagattga ccagcagcac cttgacctcg ccgcgatagt cgctgtcgat
      541 ggtgcccggc gtgttgaggc aggtgatgcc gtgcttcaag gcaaggcccg agcgcggcct
      601 aacttgacct tccatgcctt gcgggatttc cacggcaagg ccggtgggaa ccagcgcccg
      661 cctgcccggc aggatcagca tggggcggtc gtcttccacg gcggcgcgca aatccatgcc
      721 ggccgcgccc gcgctctcat agacgggcaa tggaaggccc gccccatgcg gcaggcggat
      781 gaagcccacg gcggggccga tgacggatgt tccttggggt gtcgctgaca tggcaaacct
      841 atcggacgaa ggttcggaac ggtcaatcgc cgggcggacc tatcaacggc tttcttcggt
      901 cggcataacc cgatgcgcgg cagcagccgg cgggctgatg ccggcaagcg ggcctgcggg
      961 caggacttcc gtttccgttc gacacaagag cggcgggctg gtaaaagccg gcgcatcaac
     1021 ggattcccga aatgaccgag acgataaaag acgcggtgcc gcgccgccgc accttcgcga
     1081 tcatcgccca cccggacgcc ggcaagacga cgctgaccga aaagctgctt ttgttcggcg
     1141 gcgccatcca gcttgccggc gaggtcaagg ccaagaagga ccgcatccag acgcgatccg
     1201 actggatgaa gatcgagcgc gagcgcggca tttcggtggt cacctcggtg atgaccttcg
     1261 aatatgagga caatgtcttc aacctgctgg acacgcccgg ccacgaggat tttgccgacg
     1321 atacctaccg gacgctgtcg gcggtcgatt cggcggtgat ggtcatcgac ggcgccaagg
     1381 gcatcgagcc gcgtacgctg aagctgttcg aggtctgccg gctgcgcaac attcccatcg
     1441 tcaccttcgt caacaagatg gaccgcgagg cgcgcgacac gttcgaaatt ctcgacgaga
     1501 tcgagcagaa actggcgctc gacaccgcgc cgatcacctg gccgatcggg cgcggcaaga
     1561 attttgccgg cacctaccac ctggcccaga acgctgtgcg ccggggcgat gccgagaggg
     1621 accgcacgcc ggtcaacggg cctgactcca accgcgttgc cgggcttttg ccggaaaacg
     1681 agcgcgaggc cttcatcgag gaactggacc tggcgcgcga ggcctgccgg cccttcgacg
     1741 tcgacgcctt tcgcgaaggg catatgacgc cggtttattt cggttcggcg ctgcgcaatt
     1801 tcggcgtgcg cgacctgatc gaggcgctgg gcgcctacgg gccgccgccg cgcgcgcagg
     1861 aagccgacac ccgcaccgtc gaagcgacgg aagacaagat gacctccttc gtcttcaaga
     1921 tccaggccaa tatggacccc aaccaccgcg accgcatcgc cttcgtgcgc gtctgctccg
     1981 gcaggctgga gcgcggcatg aaggccaagc tggtgcgcac cggcaagccg atgagcctgt
     2041 cggcgccgca attcttcttc gcgcgttcgc gcatcaccgc cgacgaagcc tatgccggcg
     2101 acgtggtcgg cattcccaac cacggcacgc tgcgcatcgg cgatacgctg accgagggcg
     2161 aggaactgtt gtttcgcggc gtgccgaact tcgcgccgga aatcctgcgg cgcgtgcgac
     2221 ttggcgacgc catgaaggcc aagaaactga aggaagctct gcaccagatg gccgaggaag
     2281 gcgtggtgca gctgttttcg ccggaggacg gctcgcccgc catcgtcggc gtcgtcggtg
     2341 cgctgcaact cgacgttctg aaggagcggc tcaacatcga atatacgctg ccggtggatt
     2401 tcgaaatgtc gcgcttttcc gtgtgccgct ggatttcagc cgacgacaag gcagaggttc
     2461 accgcttcat cgaggcgcat cgcggcgata tcgcccgcga cctcgacaac gacccggtgt
     2521 ttctggcgca acacgccttt tcgctcaatt acgaggccga gcgctggaag gcgatccgct
     2581 tcaccgacgt caaggactat caggtgcggg acgcggcgta gccgttttct tgccccttgg
     2641 cctactctct ttcctagccc ctggcctcag ccatttccgg ggcggctccc gccacgcggt
     2701 cgccgccgag ccatgaggtg atggcggcga tgacgtagag aatggccgag aagctgaagg
     2761 cgaagacgag gccgtgcttg aacggcccgg ccatcaggtc gggaaagaac gtgttgccgg
     2821 taagggtcgc cacgctttgc gccggcaggg cggcaagcac attggcgggg atcaattcgc
     2881 ccatcggatt atagccgagg aaggccgcaa acaggctgga aaccggcggc agattggcga
     2941 cctgatgcgc gatgtcggcc ggcacatgct gggccagcag gccggcttcc atcgcctgcg
     3001 gcaggctgga ggccaggccg agaagcatca gcgaaaagaa caggccgatc gacagcacct
     3061 ggccgacatt ggtggtggtg gcgcgcatgc cggacgcctg cccgcgctcg gcggcaggca
     3121 cggagttcat gatctgcgtg gagttcggcg cgacgaacag gccggagccg aggccgttga
     3181 ggaacagcag ggcggcgaag atggtgtagg agaaatccgc cggcaacgcc atcagcgcga
     3241 cgaagctcgc cgcgccggcc agcatgccgc cgaccgcgaa cggcacgccg ccgaagcggt
     3301 cggacagata gccggaggcc gggccggcga tgaggaagcc gaccgtcagc ggcagcatga
     3361 agatggccga ccacaagggc gtctgttcga aggaatagcc gtgcatcggc aaccagatgc
     3421 cttgcagcca cacgatgagc atgaattgca ggccgccgcg cgccacgccg gacacgaggt
     3481 tggcgatgtt gccataggcg aaggcgggaa tgcgaaacag ccgcaggtcg agcatcggcc
     3541 gggcgacgcg gcgctccacc accacgaagg cgatcagcga caggatgccg atggcgaaaa
     3601 gcgtcagcac cttgggactg gtccaggcca tggtctgctg gccatagggc tggaggccgt
     3661 aagtgatggc ggtgaggatg aggacgaggc caaggccgaa gctgatattg ccgatccagt
     3721 cgatcgtgtg tttcttgcgg tcgggcgcgt catgcagctt ccagtaggcc cagatggtgc
     3781 cggcaaggcc gaacggcacg ttgatccaga ataccagccg ccagttggta tcggcgagca
     3841 gaccgccgat gagcaggccg aggaactggc cgccgatggc cgccaccaca ttgatgccga
     3901 gcgcaaagcc gcgctcctcc accggaaacg cgtcggtgag cagcgcggtg gaattcgcca
     3961 tcaacagcgc gccgccgaca ccctgaacga tgcggacgct gaccagatag acggcggcga
     4021 aatcgccctg ccccggcagc agcccaagcg ctatcgaggc aagcgtgaag atggcgaagc
     4081 ccagattgta cattttcgcg cggccgaact ggtc
//
LOCUS       DASYCS010000161         5720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_470199,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000161 DASYCS010000000
VERSION     DASYCS010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 5720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5720
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470199"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1897
                     /locus_tag="VGM46_05205"
     CDS             <1..1897
                     /locus_tag="VGM46_05205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY4192020.1"
                     /translation="AEIFFPELTEWQSVLARLSVPLQTAVEIAVRARSDASDFQSAML
                     ACGVVTHSSLLRAVADDLGIGIVDVVDPDKLVLDGEQAIALLRSRNGQAPVKLVGSGG
                     GIVFLITPERLQLDAMRKRIAAQPMLARFLRMADPQILRDALLERARPELARTAKNSL
                     FERFPHLSARIVANAWQGSLAGALVTALPVALLLARDSLLSVAHIAATFFFFACIALR
                     FAALASFRPAAARVPVKMPDGEKPVYSVLVALYREAALIPDLLASLDRLVWPRGRLDI
                     KLVCEADDIGTLAAIRAGGLPAHIELVEVPKGGPRTKPKALAYALQTARGEFVVLYDA
                     EDDPHPMQLVDAWLRFRMSGPDLAAVQAPLEISNRHDSLVARLFAFEYAGLFRGMLPW
                     LSSRRLMLPLGGTSNHFRRTALEAVGGWDPFNVTEDADLGMRLARFGYRTEVISCPTR
                     EPAPKRFGIWLPQRTRWFKGWSQTWLVHMRDPFRLMREIGFKSFLIAQVLFAGMLASA
                     LLHPLLLVTFVVLTVQLRIDHHLGTLHSLLFIFDVVNIACGYLSFLLLGWQTLTKAER
                     KDFWKIVLFTPPYWVMLSLAGWRALWHLLRRPHHWEKTPHEPNRQGRSGRAGLRPSPP
                     EIRGLRR"
     gene            complement(1863..2759)
                     /gene="ppk2"
                     /locus_tag="VGM46_05210"
     CDS             complement(1863..2759)
                     /gene="ppk2"
                     /locus_tag="VGM46_05210"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HEY4192021.1"
                     /translation="MKKPKHGSAPTGPLKIKVRGKEREFDIDDPKLPDWIENHKLTTG
                     GYPYDKKMKGGEYDAELEALQIELVKALSWLQSAGHRVLALFEGRDAAGKGGTISALR
                     EYMNPRTARNVALTKPTELESGQWYFQRYVTHFPTKGEFVTFDRSWYNRAGVEPVMGF
                     CTPVQYEQFLEEAPHFERMIHNEGIHFFKFWLNIGQETQLDRFHDRRHSPLKNWKFSP
                     IDIAGLSKWDAYTKARDVMIERTHAADAPWIVVRANDKRRARLAVIRRILLSLPYEGR
                     DLDVIGKEDGKIIGEGPEFLED"
     gene            2884..4026
                     /locus_tag="VGM46_05215"
     CDS             2884..4026
                     /locus_tag="VGM46_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006199953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY4192022.1"
                     /translation="MSSAEDSRHWLPRALGLVRGKVKPEAFGEGRIKADAPAIQSLSG
                     EYLAAAVTDPLIIFDANALLVHANAAAFAAFGGIVPGMSLSLRFRVPEMQALLDGILS
                     GAMDTHAVDYTEKLPVERAYRVSASSVGRSTGLYVLVFKDQSETRRIDRMRADFIANA
                     SHELRTPLASIAGFIETLRGPARNDAVAREKFLAIMQEQTNRMARLIDDLLSLSRLEM
                     KPYLAPGTEVDVAKVMADVIDSLAPLASDNGIAVERQVAGDIAPVPGNRDELFQVFEN
                     LFENACKYGQSGGRVVVSIAAGSDGPVPTVDVTVRDFGPGIAEEHIPRITERFYRVDA
                     ESGRPRKGTGLGLAIVKHILTRHKATLSVWSELGQGAAFTVHLPMQ"
     gene            4142..4867
                     /gene="phoU"
                     /locus_tag="VGM46_05220"
     CDS             4142..4867
                     /gene="phoU"
                     /locus_tag="VGM46_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010913390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEY4192023.1"
                     /translation="MQSAHTASAYEGELKDLTKRIAAMGGHAERMVDQAMSALLHADP
                     GLAQKVVKDDVFLDEGQREIDDKAIIIIARRQPMATDLREIIGAIRIAADLERVGDLA
                     KNVAKRVAAVSDERQSTSLFRGIEALATLALAQLKEVLDVYASRSVDKIGFVRDRDNQ
                     IDAMYTSLFRELLTYMMEDPRNITPCTHLLFCAKNIERIGDHATNIAETIYYIVTGEQ
                     MPAERLKGDKTDKMVMPAKQGAS"
     gene            4963..>5720
                     /locus_tag="VGM46_05225"
     CDS             4963..>5720
                     /locus_tag="VGM46_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HEY4192024.1"
                     /translation="MEYARSITPAAPAPGIIASSVYAGGKRIADISVEEAGLWAKKEG
                     HVVWIGLLEPDRALLRRVQAQFALHDLAIEDAEHPHQRPKIEQYGDALFIVARTAQLV
                     EGRVTFGETHLFVGAGYIVSVRHGPSTSYTAVRQHWESCPHSLAKGEDFVLYAILDFI
                     VDNYMPVLEQIEDEVEAIEDKVLLKPMSPGDIERLYMLRRDLLRLRNAALPLVEVCRR
                     LTSSDLPQIHAAMHPLFRDVTDHIRTVQEKIDSLR"
BASE COUNT          985 a         1832 c         1792 g         1111 t
ORIGIN      
        1 ggccgaaatc ttctttccgg aactgactga gtggcaatcg gtcctggcaa ggctttccgt
       61 gccgctccag accgccgtgg agattgccgt gcgcgcgcga agcgacgcca gcgattttca
      121 atccgccatg ctggcttgcg gcgtcgtcac gcatagttcg ctgcttcgcg ccgtggcgga
      181 tgatctcggc atcggcatcg tcgatgtggt cgacccggac aagcttgttc tcgacggcga
      241 acaggctata gcgctgctgc gcagccgcaa tgggcaggct cccgtcaagc tggtgggcag
      301 cggcggcggc atcgtcttcc tgattacgcc cgagcggctg caactcgacg ccatgcgcaa
      361 acgcatcgcg gcccagccga tgcttgcacg gttcctgagg atggcggacc cgcaaatcct
      421 gcgcgacgca ttgctggagc gcgcccgtcc ggaacttgca agaacggcga agaacagcct
      481 gtttgaacgt tttccgcatt tatcggcgcg catcgtcgcc aatgcctggc aaggctcgct
      541 ggccggtgcg ttggtgaccg ccctgccggt cgccctgttg ctggcccgcg atagcttgct
      601 gtcggtcgcg cacatagcgg cgaccttttt cttcttcgcc tgcattgccc tgcggtttgc
      661 cgcattggcg tcattccggc cggcggcggc gcgggttccc gtcaagatgc ccgacggtga
      721 aaaaccggtc tattccgtgc tggtggcgct ctaccgggag gcggcgctga tccccgacct
      781 gctcgcctcg ctcgaccgac tggtatggcc gcgcggccgg ctggacatca agctcgtctg
      841 cgaagccgac gacatcggca ccctggcggc catccgcgcc ggcggtttgc cggcccatat
      901 cgaactggtt gaagtgccga agggcgggcc gaggaccaag ccgaaggcgc tggcctatgc
      961 cttgcagacg gccaggggcg aattcgtcgt gctttatgac gccgaggacg atccgcaccc
     1021 gatgcagctt gtcgacgcat ggctgcgttt ccgcatgagc ggccccgatc tggcggcggt
     1081 tcaagccccg ctcgaaattt ccaaccgcca tgacagcctt gtcgcgcggc tgttcgcctt
     1141 cgaatatgcc ggattgttcc ggggaatgct gccatggctg tcgagccggc gtttgatgct
     1201 gccgcttggc ggaacatcga accattttcg gcgcacggcc ctcgaagcgg tcggcggatg
     1261 ggacccgttc aacgtcacgg aagatgccga tctcggcatg cgtctggccc ggttcggcta
     1321 ccgaaccgaa gtcatttcat gcccgacgcg ggagcctgcg ccgaaacggt ttggcatctg
     1381 gctgccgcag cgtacgcggt ggttcaaggg ctggtcgcag acatggctgg tccacatgcg
     1441 cgacccgttc cgcctgatgc gcgagattgg tttcaagtcg ttcctgatcg cgcaggtcct
     1501 gtttgccgga atgctggcct cggcgctgtt gcacccgctg ctcctcgtca cgttcgtcgt
     1561 tttgaccgtt caacttcgga tcgatcatca cctgggcacg ctgcactcgc tgctgttcat
     1621 cttcgatgtg gtcaacatcg cctgcggcta tctttcgttc ctgcttctgg gttggcagac
     1681 gctgacaaag gccgagcgca aggatttttg gaagatcgtc ctgttcacgc caccctattg
     1741 ggtcatgctt tcgctggccg gctggcgcgc tctctggcat ctgctgcgac gcccgcatca
     1801 ctgggaaaaa accccgcacg agccgaaccg ccagggcagg tccggccgtg cgggcctgcg
     1861 ccctagtcct ccagaaattc ggggccttcg ccgatgatct tgccgtcttc cttgccgatg
     1921 acatcgaggt cgcgtccttc ataaggcagc gacagcagga tgcgccggat gacggccagc
     1981 cgggcgcggc gcttgtcgtt ggcgcgcacg acgatccacg gcgcgtcggc cgcatgggtg
     2041 cgctcgatca tcacgtcgcg cgccttggtg taggcgtccc acttgctcag gccggcaatg
     2101 tcgatgggcg aaaacttcca gttcttcagc gggctgtgcc ggcggtcgtg aaagcggtca
     2161 agctgcgttt cctgcccgat attgagccag aacttgaaga aatggatgcc ctcattgtgg
     2221 atcatccgct cgaaatgcgg cgcttcctca aggaattgtt cgtactggac aggcgtgcag
     2281 aaacccatca ccggctcgac gccggcccgg ttgtaccagg agcggtcgaa ggtgacgaat
     2341 tcgcccttgg tcgggaaatg cgtgacgtaa cgctggaaat accattgccc gctttccagt
     2401 tcggtgggct tggtcagcgc cacgttgcgc gccgtgcgcg ggttcatgta ctcgcgcaac
     2461 gccgagatgg tgccgccctt gccggcggcg tcgcggccct cgaacagcgc cagaacccgg
     2521 tggccggccg attgcagcca ggacagcgcc ttgacgagtt ctatctggag cgcttccagt
     2581 tccgcatcat attcgccgcc cttcatcttc ttgtcgtatg gatagccgcc agtggtgagc
     2641 ttgtgattct ctatccagtc cggcagcttc gggtcatcga tatcgaattc ccgttccttg
     2701 ccccgaacct tgatcttcag cgggccggtt ggcgcggaac cgtgtttggg ctttttcatc
     2761 gatgcgaacc tttccagttt caggcaacat gctattggga ggcccaatct cggtccagcg
     2821 ggaactttcc gataggttca aagccatgcc agatccggcg ccgtttacga atttgtggct
     2881 ccaatgagta gcgccgaaga cagtcggcac tggctgccgc gcgcgctcgg gctggtccgc
     2941 ggcaaggtca agccggaagc tttcggcgaa ggccgcatca aggccgatgc gccggccatc
     3001 cagtcgcttt ccggcgaata tctggctgcg gccgtcaccg acccgctcat catcttcgac
     3061 gcgaacgccc tgctcgttca cgccaatgcg gcggccttcg cggccttcgg cggcatcgtt
     3121 cccggcatgt cgctgtcgtt gcggtttcgc gtgcctgaaa tgcaggcgtt gctggacggc
     3181 atcctgtctg gcgcgatgga cacgcatgcc gtcgactaca ccgaaaaact gccggtcgag
     3241 cgcgcctacc gggtcagcgc ctcttcggtc gggcgcagca ccggccttta tgtcctggtg
     3301 ttcaaggacc agagcgagac gcggcggatc gaccggatgc gcgccgattt catcgccaat
     3361 gccagccatg aactgcgtac gccgctggcc tcgattgctg gcttcatcga gacgctgcgc
     3421 ggcccggccc gcaacgacgc cgtcgcgcgc gaaaaattcc tggccatcat gcaggagcag
     3481 accaaccgca tggcgcggct gatcgacgac ctgctgtcct tgtcgcgcct ggaaatgaag
     3541 ccttatctgg cgcccggaac cgaagtcgat gtcgccaagg tcatggccga cgtcatcgat
     3601 tctctcgcgc cgctggcaag cgacaacggc atcgccgtcg agcggcaggt cgccggcgat
     3661 atcgcgccgg tgccgggcaa ccgcgacgag cttttccagg tattcgaaaa cctgttcgaa
     3721 aacgcctgca aatacggcca gtcgggcggg cgcgtcgtgg tctcgatcgc ggccggttcc
     3781 gatggcccgg tgccgacggt ggatgtcacc gtgcgggatt tcgggccggg catcgccgag
     3841 gagcatattc cgcgcatcac cgagcggttc taccgcgtcg atgccgaaag cggacggccg
     3901 agaaaaggca cgggccttgg gcttgccatc gtcaagcaca tcctgacccg ccacaaggca
     3961 acgctttcgg tgtggtccga acttggtcag ggcgcggcct tcaccgttca tctgccgatg
     4021 cagtgacggc gcaaaatcga cgaccccgaa acggtttctt ttttctgtca tagggttagc
     4081 gtaagaacag gagattcaag gcaatgcatg gtcaggcatt gcgcaccggg agccaccact
     4141 catgcagtcc gcgcacaccg cgagcgccta tgaaggcgaa ctgaaagacc tgacgaagcg
     4201 catcgccgcg atgggcggcc atgcggagcg gatggtcgat caggcgatgt cggcgctgct
     4261 tcacgccgat ccgggtcttg cccagaaggt ggtcaaggac gacgtctttc tcgacgaggg
     4321 acagcgtgag atcgacgaca aggccatcat catcatcgcc aggcgccagc cgatggcgac
     4381 cgacctgcgc gagatcatcg gggcgatccg catcgcggcc gatctcgaac gggtaggcga
     4441 cctcgccaag aacgtcgcca agcgcgtggc cgccgtcagc gacgaacgcc agtccaccag
     4501 cctctttcgc ggcatcgagg cgctggccac gctggcgctg gcgcagctca aggaagtgct
     4561 ggacgtctat gcttcgcgct cggtcgacaa gatcggcttc gtgcgcgacc gcgacaacca
     4621 gatcgacgcc atgtacacgt cgctgtttcg cgaattgctg acctacatga tggaagaccc
     4681 gcgcaacatc acgccctgca cgcatctgtt gttttgcgcc aagaacatcg agcgcatcgg
     4741 cgaccacgcc accaatatcg ccgagacgat ttactacatc gtcaccggcg agcagatgcc
     4801 ggcggagcgg ctcaagggcg acaagacgga caagatggtc atgcccgcga agcaaggcgc
     4861 ttcctgatcg ccgcctggtc tcgaatatac cggctgagag cgctataatg aagccgttgc
     4921 tgcccggccc tggcggtttc caggcgcgtt gcggaggttg cgatggaata tgcccgtagc
     4981 ataacccctg ccgcaccggc ccccggcatc atcgcgtcaa gcgtctatgc gggcggcaag
     5041 cgcatcgccg acatttccgt cgaggaagcc ggcctgtggg cgaagaagga aggccatgtc
     5101 gtctggatcg gcctgctgga gcctgaccgc gctttgctgc ggcgcgtgca ggcgcagttc
     5161 gccctgcatg acctggcgat cgaggatgcc gaacacccgc accagcgccc caagatcgag
     5221 caatatggcg atgcgctgtt catcgtcgcg cgcaccgcgc aactggtcga ggggcgcgtc
     5281 accttcggcg aaactcacct gttcgtcggc gccggctaca tcgtcagcgt tcgccacggg
     5341 ccgtcgacct cctacacggc cgttcgccag cactgggagt cctgcccgca ttcgctggcc
     5401 aagggcgagg atttcgtcct ctacgccatc ctcgatttca tcgtcgacaa ttacatgccg
     5461 gtgctggagc agatcgagga cgaggtcgag gcgatagagg acaaggtgct gctcaagccg
     5521 atgtcgccgg gcgacatcga gcggctctac atgctgcggc gcgacctgtt gcggctgcgc
     5581 aatgccgctc ttccgctggt ggaggtctgc cgccggctga ccagctcgga cctgccgcaa
     5641 atccacgccg ccatgcaccc gctgtttcgc gacgtgacgg accatatccg caccgtgcag
     5701 gaaaagatcg acagcctgcg
//
LOCUS       DASYCS010000162         5962 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2790862,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000162 DASYCS010000000
VERSION     DASYCS010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 5962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5962
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2790862"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..1219
                     /gene="exbB"
                     /locus_tag="VGM46_05230"
     CDS             167..1219
                     /gene="exbB"
                     /locus_tag="VGM46_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tonB-system energizer ExbB"
                     /protein_id="HEY4192025.1"
                     /translation="MQPASSIRQAPRTQPAPLAPSATPIRPVPPTQSVPPARPAANIQ
                     PTPSTQPAPASGAQQPAAVAQPAPGGVSDNAPNINPDSTIEISEASLPRNLSPWGMFA
                     AADWVVKGVMIGLAFASLLTWTIWLAKSLEIAGAKTRLRKALRAIVGSASLQEASQKL
                     DRTGGAGALLVAAAKEEARLSAGALDYADPAGLKERVASHLSRIMAQAGRRLGRGTGI
                     LATIGSTAPFVGLFGTVWGIMNAFIGISKAQTTNLAVVAPGIAEALLATALGLVAAIP
                     AVVIYNVFARSVTGYRQALADAAAGVERLVSRDLDYRLAPPGDNGRAASATLFPAPAI
                     RAPAIRAPETHADAAA"
     gene            1229..1699
                     /gene="exbD"
                     /locus_tag="VGM46_05235"
     CDS             1229..1699
                     /gene="exbD"
                     /locus_tag="VGM46_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002967880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HEY4192026.1"
                     /translation="MAGGIREAVDDDLLETHEINVTPFIDVMLVLLIIFMVAAPLATV
                     DVNVDLPASSATPSPRPDKPLYLTVKQDLAVMLGNEPVPREALAGALDRLTQGDRQQR
                     VFLRADQTVGYGDLMDVMNLLRKAGYLKIALVGLETTPDAAALAAAPGATVSPR"
     gene            1696..2628
                     /locus_tag="VGM46_05240"
     CDS             1696..2628
                     /locus_tag="VGM46_05240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEY4192027.1"
                     /translation="MSERRIYPADSLSLDLGGRDIALWALAAMLVLSAHLASAWYLQR
                     MPPALPASAQAAPAIMMDLAPMMTAPDAARSDVAETVNSATADPVEEPTEKPEPAKEP
                     AVQEAARDEPVEKAEAAEAEPLEEQAPQPAKQAEEVVPDLQEAPLPEVALAIPQPRPV
                     AKKPVEEPVKKPVAKPAKKKTVKVERKAPPPQMAAVKTAEKAAKEAAPRVKITAAGTG
                     ISPARWQSRVNAHLNRYKRYPNGGHGRGTVTVRFTINESGGVTSAALSGSSGDAAFDT
                     AALDLVRRASPVPAPPPEIARPRMSLVVPIYFSR"
     gene            2694..3686
                     /locus_tag="VGM46_05245"
     CDS             2694..3686
                     /locus_tag="VGM46_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020094382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEY4192028.1"
                     /translation="MNGEIIFIGAGGTIGSLGRDPHDILHYHSTGNRLSAAEILNSLP
                     ASPVLSSVEPVEFRNLDSTAVTAKDWRDLAGLCERLAAEKPSLAGIVIGHGTASLEET
                     AFCLSLTMKLAIPVVVTGAMRPLNGISSDAARNLVAAIHVARSARSAGRGVLVVFNDE
                     IHAAREVTKTSTSRLDAFRSPDRGPLGEVRGDEAVFYRRSERRHMADSEFSVEDIENM
                     PRVDIVYSHAGADDTAIRAFVDAGAKGIVSAGFGPGVGTPAEIAGFEWAMARGVAVVQ
                     SSRTGGPALDTEPQHTMGIVTTDNLTPQKARILLGLALSKTTDRAQLRRIFETY"
     gene            complement(3842..5134)
                     /gene="rhaI"
                     /locus_tag="VGM46_05250"
     CDS             complement(3842..5134)
                     /gene="rhaI"
                     /locus_tag="VGM46_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019863186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose catabolism isomerase"
                     /protein_id="HEY4192029.1"
                     /translation="MIEAMIAADIIARENDAREKDLRRDYETLGERLDRRGIAIDAIR
                     DKVAAFHVAIPSWGVGTGGTRFARFPGEGEPRDIFDKIEDCAVIQQLTRATPNVSLHI
                     PWDKADPNRLKQAASRFGLGFDAMNSNTFSDAKDQQRSYKFGSLSHADAGTRRQAVEH
                     NLECIEIGQAIGSKALTVWIGDGSNFPGQVNFARAFERYLDAMRDIYAELPDDWRLFS
                     EHKMYEPAFYSTVVQDWGTNYIIARELGPKALCLVDLGHHAPNVNIEMIVSRLVQFGK
                     LGGFHFNDSKYGDDDLDAGSIDPYRLFLVFNELVDAELKSAEGFDPAHMLDQSHNVTD
                     PIESLMLSAIEVQRAYAQALLVERVALEGFQESNDALMATQTLKTAYRTDVEPILAMA
                     RMMAGGAVDPVATYRTAGYRTRVAAERPATAAGSGGIV"
     gene            complement(5243..>5962)
                     /locus_tag="VGM46_05255"
     CDS             complement(5243..>5962)
                     /locus_tag="VGM46_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018094193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY4192030.1"
                     /translation="GACVVLADIDEAALAKASDEFAAAYGKDFVRSVRVDVTAEEQVV
                     SGFASTAVEFGGIDILVSNAGLASSAPIEETTLALWNRNMDILATGYFLVSREAFRLF
                     RTQKIGGNVVFVASKNGLAASPNASAYCTAKAAEIHLARCLALEGAEAQIRVNTVNPD
                     AVLRGSKIWTGEWKEQRAAAYKMSTDDLEEHYRSRSMLKRSVFPEDIAEAIYFFASDM
                     SAKSTGNIVNVDAGNAQSFTR"
BASE COUNT         1026 a         2009 c         1933 g          994 t
ORIGIN      
        1 cgcaccggca aggcaggcgc tggccgccgc gatggtgctg atgcttgccg gcggcgcgtt
       61 ttgcggcatc gacgccaagg cgcaacaaca gcgcgacgcg gcagagaagg ggagttcgac
      121 cccctccgcc gccccgatag cggagccggc ctcatccgcg cccgccatgc aaccggcatc
      181 gtccatccgg caagcgcccc gcacacagcc tgcgccgctt gccccgtcag ctactcccat
      241 ccgaccagta ccacctaccc agtcagtgcc gccggcacgg cctgctgcca acattcagcc
      301 tacgccctcc acccagccag cgcccgcgtc cggcgcgcag caacccgccg cagtcgcgca
      361 gccagcgccc ggcggcgtct cggataacgc gccaaacatt aacccagaca gcaccatcga
      421 aatcagcgaa gcctccctgc cgcgcaacct gtcgccgtgg ggcatgttcg ccgccgccga
      481 ctgggtggtc aaaggcgtca tgatcggcct tgcctttgcc tccctgctga cctggacgat
      541 ctggctggcc aagtcgctgg aaatcgccgg cgcgaagacg cggctgcgca aggcgctgcg
      601 cgcgatagtg gggtcggcct cgctgcaaga agcgtcgcaa aagctcgacc gcaccggcgg
      661 cgcgggcgcg ctgctggtcg cggcggcgaa ggaggaagcc cggctttcgg ccggtgcgct
      721 cgactatgcc gatccggccg gattgaaaga gcgcgtcgcc tcgcatctgt cgcgcatcat
      781 ggcgcaggcc ggccgccggc tcggccgcgg caccggcatc ctggccacca tcggctcgac
      841 cgccccgttc gtcggcctgt tcggcaccgt ctggggcatc atgaacgcct ttatcggcat
      901 ttcgaaggcc cagaccacca atctcgccgt cgttgccccc ggcatcgccg aagcccttct
      961 ggcaacggcg ctcggcctcg tcgccgccat tcccgccgtc gtcatctaca atgtcttcgc
     1021 acgctccgtc accggctacc ggcaggcgct ggcggatgcg gcggccggcg tcgagcgcct
     1081 cgtcagccgc gatctcgact accggctggc cccgcccggc gacaatggcc gcgccgcctc
     1141 ggcgacgctg tttcccgcgc ccgcaattcg cgcgccggca attcgcgcgc cagaaaccca
     1201 cgcggatgcg gccgcatagg aggcgacgat ggctggaggc atcagggaag cggtcgatga
     1261 cgaccttttg gaaacccacg aaatcaacgt cacacccttc atcgacgtca tgctggtgct
     1321 gctcatcatc ttcatggtgg ccgcgccgct ggccacggtc gacgtcaatg tcgacctgcc
     1381 cgcatcctcg gcaacgccgt cgccacggcc cgacaagccg ctctacctga ccgtcaagca
     1441 ggaccttgcc gtcatgctgg gcaacgagcc ggtgccgcgc gaggcgctgg ccggcgcgct
     1501 cgaccgcctg acgcagggcg accggcagca gcgcgtcttc ctgcgggcgg accagacggt
     1561 cggctatggc gatctgatgg atgtcatgaa cctgctgcgc aaggcgggct atctgaagat
     1621 cgcccttgtc ggccttgaaa cgacaccgga tgccgctgca ttggccgccg ctccgggcgc
     1681 aaccgtttcg cctcgatgag cgagcggcgc atatatccgg ccgacagcct gtcgctcgac
     1741 ctcggcgggc gcgacatcgc gctgtgggcg ctggcagcca tgctggtgct gtcggcgcac
     1801 ctggccagcg cctggtatct ccagcggatg ccgcctgcct tgccggcatc ggcgcaggca
     1861 gcgcccgcca tcatgatgga cctcgcgccg atgatgaccg cgccggatgc ggcccgctcg
     1921 gacgtggccg agacggtgaa cagcgccaca gccgatccgg tagaggaacc aaccgagaag
     1981 ccggagccgg ccaaggagcc ggccgtgcag gaagcggcgc gggacgaacc cgtcgagaaa
     2041 gcggaagccg ccgaagccga gccgctggag gaacaggcgc cgcagccggc gaaacaggcc
     2101 gaggaggtcg ttcccgactt gcaagaagcg ccgctgccgg aagtggcgct ggccataccg
     2161 cagccccggc ccgtcgcgaa aaagccggtt gaggagccgg tgaagaaacc ggtcgcgaag
     2221 ccggctaaga aaaagaccgt caaggtcgag cgcaaggccc cgccgccgca gatggcggcg
     2281 gtgaagacgg ctgaaaaggc ggcgaaggaa gcagcgccca gggtcaagat cactgctgcc
     2341 ggcaccggca tctcgccggc gcgctggcag tcgcgcgtca acgctcatct caaccgctac
     2401 aagcgctatc ccaacggtgg ccatgggcgc ggtacggtca ccgtgcgctt caccatcaat
     2461 gaaagcggcg gcgtcacctc ggcggcgctg tcgggttcgt cgggcgacgc cgccttcgat
     2521 acggcggcgc tcgatctggt gcgccgcgcc tcgccggtgc ccgcgccgcc gcccgaaatc
     2581 gcccgcccgc gcatgagcct ggtggtgccg atctattttt cgcgctgaag cctgggactt
     2641 gtccggcccg acgctgctat accgcaccgt cgggcggcgg cggggaagca caaatgaacg
     2701 gcgaaatcat cttcatcggg gccggcggaa ccatcggttc gctgggccgg gacccgcatg
     2761 acattctcca ctaccactcg accggcaaca ggctgtcggc ggcggaaatc ctgaacagcc
     2821 tgccggcatc gccggtcttg tccagcgtcg agccggtcga gttcaggaac ctggactcga
     2881 ccgccgtcac cgccaaggac tggcgcgatc tggccggcct gtgcgaacgg ctggcggcgg
     2941 aaaaaccatc attggcgggc attgtcatcg ggcatggcac ggcaagcctg gaagagaccg
     3001 cgttttgcct gtcgctgacg atgaagctcg ccatccccgt cgtcgtcacc ggcgcgatgc
     3061 ggcccttgaa cggcatttcc tccgatgccg cccgcaacct cgtcgccgcc atccatgtcg
     3121 cgcgcagcgc gcgctcggcg gggaggggcg ttctcgtcgt tttcaacgac gaaatccacg
     3181 ccgccaggga agtgaccaaa acctcgacct caaggctgga cgcttttcgc agcccggatc
     3241 ggggaccgct gggtgaagtt cgcggcgatg aagcggtatt ttaccggcgc agcgaaaggc
     3301 ggcacatggc ggacagcgag ttttcggtcg aagacatcga aaacatgccc cgcgtcgata
     3361 tcgtctattc gcatgccggc gcggacgaca ccgccatccg cgcctttgtc gatgccggcg
     3421 ccaagggcat cgtctcggca ggcttcggcc ccggcgtcgg cacgcctgcc gaaatcgccg
     3481 ggttcgaatg ggctatggcc agaggcgtcg cggtggtgca gtcgtcgcgc accggcggcc
     3541 ccgcgctcga tacggagccg cagcatacca tgggcatcgt gaccaccgat aatttgaccc
     3601 cgcaaaaagc ccgcatcctg cttggcctgg cgctttccaa aacaaccgac cgcgcgcaac
     3661 tgcgccggat attcgagact tattgagggc cggcgggtcg ttttcctgaa ctgccggcgc
     3721 gtctacaagc catgccggcg atcactgcaa aacgcgcaac cgccaattat ctgcggtgtt
     3781 ccggcgcgtg tcgtcggccg gctccccgcc gcgattgccg tggatcgaaa cggcggggcg
     3841 cttagactat gccgccgctg ccggctgcgg ttgccgggcg ctcggccgct actctggtgc
     3901 ggtagccggc ggtgcgatag gtcgccaccg ggtcgacagc gccgccggcc atcatgcgcg
     3961 ccatcgccag tatcggctcg acatcggtgc ggtaggccgt cttcagcgtc tgcgtcgcca
     4021 tcagcgcgtc gttgctttcc tgaaagccct caagcgccac gcgctcgacc agcagcgcct
     4081 gcgcgtaggc gcgctgcacc tcgatggccg acagcatcag gctttcgatc gggtcggtga
     4141 cgttgtggct ttggtccagc atgtgggccg gatcgaagcc ttcggcgctt ttcagctcgg
     4201 catcgaccag ttcgttgaag accaggaaca gccggtaggg atcgatcgat ccggcgtcga
     4261 gatcgtcgtc gccatatttg gaatcgttga aatggaaccc gcccagtttt ccgaactgga
     4321 cgagccgcga aacaatcatc tcgatgttga cgttcggcgc gtggtggccg agatcgacaa
     4381 ggcacaaagc cttggggccg agttcgcggg cgatgatgta attggtgccc cagtcctgca
     4441 ccaccgtcga atagaaagcc ggctcataca tcttgtgttc ggaaaacagc cgccagtcgt
     4501 cgggcagttc ggcatagatg tcgcgcatcg catcgagata gcgctcgaag gcgcgggcga
     4561 agttgacctg gcccggaaaa ttcgagccgt cgccgatcca caccgtcagc gctttcgagc
     4621 cgatggcctg gccgatttcg atgcattcca gattatgctc gacggcctgg cggcgcgtgc
     4681 cggcatcggc atggctgagc gagccgaact tgtaggagcg ctgctggtcc ttcgcgtcgg
     4741 aaaatgtgtt ggaattcatg gcgtcgaagc cgaggccgaa gcgcgatgcc gcctgcttca
     4801 gccggttcgg gtcggccttg tcccatggta tgtgcagcga aacgttcggc gtcgcccgcg
     4861 tcagttgctg gatgacggcg caatcctcga tcttgtcgaa aatatcgcgc ggctcgcctt
     4921 cgccgggaaa gcgcgcaaag cgcgtgccgc cggtgccgac gccccaggag gggatcgcga
     4981 catggaaggc cgccaccttg tcgcgaatgg cgtcaatggc gatgccgcgc cggtccagcc
     5041 gctcgccaag cgtttcgtag tcgcggcgca ggtctttttc gcgggcgtcg ttctccctgg
     5101 cgatgatatc ggcggcgatc atggcttcga tcatgcggtc ctcccggcgg cctttgccct
     5161 ctcccttttt ttggggaggg tcggcgaaca aactgtcgct acgctcaagc atcgacctcc
     5221 ccgcaagggg gagggtaagg ccctagcgcg taaagctttg cgcgttgccg gcatcgacat
     5281 taacgatgtt gccggtcgat tttgccgaca tgtcggaggc gaagaaatag atcgcctcgg
     5341 caatgtcttc ggggaagacc gaacgcttca gcatggagcg cgagcgatag tgttcctcaa
     5401 gatcgtcggt ggacatcttg taggcggcgg cgcgctgttc cttccattcg cccgtccata
     5461 tcttggagcc acgcaagacg gcatcggggt tgacggtgtt gaccctgatc tgcgcttcgg
     5521 cgccttccag cgccaggcag cgcgcgaggt ggatttcggc ggctttggcc gtgcaatagg
     5581 cggaagcgtt gggcgaggcg gcaaggccgt tcttggaagc gacgaaaacg acgttgccgc
     5641 cgatcttctg ggtgcgaaac agcctgaagg cttcgcggct gaccaggaaa tagccggtcg
     5701 ccagaatgtc catgttgcga ttccacaatg ccagcgtggt ttcctcgatg ggagccgatg
     5761 acgccagccc cgcgttggaa accagaatat cgatgccgcc gaactccacc gccgtgctgg
     5821 cgaaaccgga aaccacttgt tcctcggccg tcacgtccac ccgcaccgag cgcacgaaat
     5881 ccttgccgta ggcggctgcg aattcgtcgc tggccttggc gagcgccgcc tcgtcgatat
     5941 cggccagcac cacgcaggcg cc
//
LOCUS       DASYCS010000163         3657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2067185,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000163 DASYCS010000000
VERSION     DASYCS010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2067185"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(309..>3657)
                     /locus_tag="VGM46_05260"
     CDS             complement(309..>3657)
                     /locus_tag="VGM46_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HEY4192031.1"
                     /translation="EIAQGETAKIGVDGRFLYGAPAVGLALEGELTLSTTRDWDRYKG
                     FSFGLADEQAAEPTVTPFADLPLVGDDGKAEFPVSVDQLPATTKLVNTKVTVRMRETG
                     GRAVERSLDIGVRPQTPMIGIRPDFSGDEVPQGGTAKFSLIVVDKDGNREALKGASWS
                     LVKVERNYQWYRSGGSWRYEAVAFTKAAASGTVDIAADGEATVSLPVDWGQYRLEVET
                     ADPEGPASSYEFDAGWYVSASSTETPDGLEIALDKDSYATGDTAKLKISPHFAGELLV
                     TIGADKLLKTVTATVPEAGATIDVPVGEDWGAGAYVTATLYRPGDAQESRMPTRAIGV
                     KWLKVDPGAKKLAVTLTPPEKTTPRQTLSVPVAVAGVQPGTQAYVTVAAVDVGILNLT
                     NYKAPDPEGWFFGQRMLGLELRDLYGLLIDGSLGTTGKLRTGGDGANMQAQGAPPTEK
                     LVAFFSGIVELDAEGKATVDFDIPQFNGTVRVMAVAWTKDAVGHAQSDVIMRDPVVIT
                     AGLPRFLAPGDAAIMRLDVANTDGPDGDYAVAIESTGGLSIASDGVPGSLTLTGGKRQ
                     SFTVPLIAVTPGDASVTVKLSHEGGPAVEQTLLLPVRPSQLPVTTRMVVDLKPNGGSL
                     RIDKELLAASLLDGASVSVGVSQAAAFDVPSLLMTLDRYPYGCSEQTTSRALPLLYLN
                     EMAAGIGMTKDPDLRQRIQDAIYKVLNNQGSSGGFGLWGPGEGDLWLDSYVADFMTRA
                     REQKYDVPENAMNQALANLQNSLGYDQDVKSRGSAMAYALYVLARNKKASIGDLRYYA
                     DTQLEAFSSPMAVAQLAAALALYGDTQRSEATFSAALRLAKASTGYDWSRSDYGSALR
                     DGAAMLSLAAETRPAPAIVPELIKLVTVERAQARWTSTQDDAWMLLAARALKSGNDAI
                     ALTVNGQPHSGAYSSEIAGSDLADSPMTIANTGGTPLQAVVTTVAAPAQPLPAGGDGF
                     TIARTYYTLDGNEANVTEATQNERYVVVLKIDELNSWPSRLLVTDLLPAGFEIDNPSL
                     VSSAQLSNFSWLAQTEAAHLEFRDDRFVAAFNPNDGGGNRSIMLAYVVRAVTPGVYAH
                     PAAVVEDMYRPQFSARTATGMMEVKAP"
BASE COUNT          615 a         1149 c         1248 g          645 t
ORIGIN      
        1 gcacaggctg aatgagggcg gtgccggggc ctccccccac ccctaacccc tccccacaaa
       61 ggggaggggg acgatgggtc gactgtcgcg gttatagttg caggttgatt gtgagcaacg
      121 gcgttaggga gttctttggc caacgtgagg gagggagagg ataagccgcc cattgcaatc
      181 ccagttgcct cgtggcgcag aacgaaggcg cggaatggtt cccctcccgc ttgtggggag
      241 ggttcacggg tgggggtatg aagcccacaa caaggcgaag aatcggtcat ctctcaaccc
      301 tcgaccctct acggcgcctt cacctccatc atgccggtgg cggtgcgggc cgagaattgc
      361 gggcgataca tgtcttccac caccgctgcc gggtgcgcgt agacgccggg cgtgaccgcg
      421 cgcacgacat aggccagcat gattgagcgg ttgccgccgc catcgttcgg gttgaaggcc
      481 gccacgaaac ggtcgtcgcg gaattcgaga tgcgccgcct cggtctgcgc cagccaggag
      541 aagttggata gctgcgcgct ggaaacgagg cttggattgt cgatctcgaa gcccgccggc
      601 agaaggtcgg tgaccagcag gcgcgaaggc cagctgttca gttcgtcgat cttaagcacc
      661 accacatagc gctcgttctg cgtggcctcg gtgacgttgg cctcgttgcc gtcgagcgtg
      721 taataggtgc gcgcgatggt gaagccgtcg ccgccggccg gcaatggctg ggcgggggcg
      781 gcgaccgtgg tgacgaccgc ctgcaacggc gtgccgccgg tgttggcgat ggtcatcggg
      841 ctgtcggcaa ggtcactgcc ggcgatctcg ctcgaatagg cgccgctgtg cggctggccg
      901 ttgaccgtca gcgcaatggc gtcattaccg gatttcaatg cccgcgccgc cagcagcatc
      961 caggcatcgt cctgcgtgct cgtccagcga gcttgcgcgc gttccaccgt caccagcttg
     1021 atcagttccg gcacgattgc cggcgccggc ctggtttccg ccgccagcga cagcatggcc
     1081 gcgccatcgc gcaacgccga gccgtaatcg gagcgggacc aatcatatcc ggtcgacgcc
     1141 ttggccagcc ggagcgccgc gctgaaggtc gcttccgaac gctgggtatc gccgtaaagc
     1201 gccaatgccg ccgccagctg cgccaccgcc atcgggctgg aaaacgcttc aagctgggtg
     1261 tcggcatagt agcgcaggtc gccgatggag gccttcttgt tgcgcgccag cacgtagagc
     1321 gcataggcca tggcgctgcc gcggcttttt acgtcctgat cgtagcccag cgaattctgg
     1381 agattggcaa gcgcctggtt catggcgttt tccggcacat catatttctg ctcgcgcgcc
     1441 cgcgtcatga aatcggcgac ataggaatcg agccagaggt cgccttcgcc cggcccccac
     1501 aggccgaaac caccgctgga accctggttg ttcaacacct tgtagatggc gtcctgaatg
     1561 cgctggcgca ggtcggggtc cttggtcatg ccgatgcccg ccgccatttc attgaggtag
     1621 agcaggggca aggcgcggct cgtcgtctgc tccgaacagc catagggata gcggtcgagc
     1681 gtcatcaaaa gcgacggcac gtcgaaggcg gcggcctgtg aaacgccgac gctgacggat
     1741 gcgccgtcca gcaggcttgc cgccaacagc tccttgtcga tgcgcagcga accgccattg
     1801 ggtttgagat cgaccaccat gcgcgtggtg accggcagtt gcgatgggcg caccggcaac
     1861 agcagcgtct gttcaacagc cggaccgcct tcatgggaaa gcttgacggt gaccgaggcg
     1921 tcgccgggcg tcaccgcgat cagcggcacg gtgaaggact ggcgcttgcc gccggtgagc
     1981 gtcaggctac ccggaacgcc atcgcttgcg atggaaaggc cgccggtgga ttcgatggcg
     2041 acagcatagt cgccatccgg gccatccgtg ttggcgacgt cgagccgcat gatggcggcg
     2101 tcgcctggcg cgaggaagcg cggcaggccg gcggtgatga cgaccggatc gcgcatgatg
     2161 acgtcggact gggcgtggcc gaccgcgtcc ttcgtccagg cgactgccat gacccgcacg
     2221 gtgccgttga actggggaat gtcgaaatcg acggtggcct tgccctcggc atccagctcg
     2281 acaatgccgg agaagaaggc gaccagcttt tcggtcggcg gcgcgccctg cgcctgcatg
     2341 ttggcgccgt cgccgccggt gcgcagcttg ccggtggtgc cgagcgagcc atcgatgagc
     2401 aggccgtaaa ggtcgcgcag ttcaaggccg agcatgcgct gcccgaagaa ccagccttcc
     2461 gggtcgggcg ccttgtagtt ggtgaggttg aggatgccga catcgacggc agcgaccgtc
     2521 acataggctt gcgtgccggg ctggacgccg gcgacggcaa ccggcaccga cagggtctgg
     2581 cgcggcgtgg tcttttccgg cggcgtcagc gtgacggcga gcttcttcgc gccggggtcg
     2641 accttcagcc atttcacgcc gatggcgcgg gtgggcatgc gcgattcctg cgcatcgccc
     2701 ggccggtaaa gcgtggcggt gacataggcg cccgcgcccc agtcctcgcc gaccggcacg
     2761 tcgatcgtgg cgcccgcctc tggaacggtt gcggtgacgg ttttgagcag cttgtcggcg
     2821 ccgatggtaa ccagaagctc gccggcgaaa tgcggcgata ttttcagctt ggcggtgtcg
     2881 ccggtggcgt agctgtcctt gtcgagggcg atttcaagcc cgtccggcgt ttcggtcgag
     2941 gaggcgctga cataccagcc ggcgtcgaat tcgtagctgg aggcagggcc ttcgggatcg
     3001 gcggtttcga cctccagcct gtactggccc caatcgaccg gcagagacac ggttgcctcg
     3061 ccatcggcgg cgatatcgac ggttccgctg gcggcggcct tggtgaaggc taccgcctcg
     3121 tagcgccagg aaccgccgct gcgataccat tgataattgc gctcgacctt gaccagcgac
     3181 caggaagcgc ccttcagcgc ttcacgatta ccgtccttgt cgacgacgat caggctgaat
     3241 ttggcggtgc cgccctgcgg aacctcgtca ccggaaaagt cggggcgaat gccgatcatc
     3301 ggggtctgcg ggcgcacgcc gatatccagc gaccgctcga cggcgcgacc gccggtttcg
     3361 cgcatgcgca ccgtcacctt ggtgttgacc agcttggtcg ttgccggcaa ctggtcgacc
     3421 gaaaccggga actcggcctt gccgtcatca ccgaccaagg gcaggtcggc aaacggcgtc
     3481 accgtcggct cggcggcctg ttcatcggca aggccaaagg aaaagccctt gtagcggtcc
     3541 cagtcgcgcg tggtggaaag ggtaagctcg ccttccagcg caaggccgac ggccggcgcg
     3601 ccatagagga agcggccatc gacgccgatc ttggcggttt cgccctgcgc gatttcc
//
LOCUS       DASYCS010000164        11225 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4094665,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000164 DASYCS010000000
VERSION     DASYCS010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 11225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11225
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4094665"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /locus_tag="VGM46_05265"
     CDS             complement(<1..996)
                     /locus_tag="VGM46_05265"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEY4192032.1"
                     /translation="MASFKTLDDIGNIAGKRVLVRVDLNVPVTDGKVSDTTRIERILP
                     TLDELSKKGAKVILLAHFGRPKGGPAPEFSLDPIAYAVSAMIGKPVAFAADCIGAEAA
                     NAVGAMKDGDILLLENTRFHKGEEKNEPDFADALAKNGDIYVNDAFSAAHRAHASTEG
                     LAHRLPAFAGRTMQAELDALEQGLGNPAKPVVALVGGAKVSTKIDLLMNLVRKVDALV
                     IGGGMANTFLAAQGKPIGKSLCEHDLIPNARDILAKAKSLKREIVLPVDAVVAQKLAA
                     HVPTRVVPIEEIAATDMMLDIGPRSVEHVVSVLARCKTLVWNGPFGAFEIEPFDIG"
     gene            complement(1085..3007)
                     /locus_tag="VGM46_05270"
     CDS             complement(1085..3007)
                     /locus_tag="VGM46_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HEY4192033.1"
                     /translation="MEHAIYLVTLVGTGLVVAAAFSSLIAFRFGAPLLLLFLGIGLVS
                     GADGLGIQFDNARVAYFAGSLALAVILFDSGFGTPLRALKQAALPALSLATVGVLITT
                     GVFGVAVFYLLNLTWLESFLLGAAVASTDAAAVFFLLRAGEINLRERVRSTLEVESGT
                     NDPIAIFLTITLVEVIAAGAHPEAKVLVTDLILGFFVNMGLGAIVGVLGGMAIVRLVD
                     RLNLDHGLLPIFVLTLSLMVFAAAGTIGGSGFLAVYLAGLVAGNSDIRAVAVLKRFQD
                     GMSWLAQIIMFLILGLFATPSQFGELLLPAMLLGLFLIFVARPLAVWLCLMPFRLPRP
                     EVAFISWVGLRGAVSILLAITPLLSGLEHGRLIFNVAFIIVLVSLVIQGWTVGPLARR
                     LGLIVPARLGPLDKVELELPGSARHELLAYKVAAGSPVARGERIPRWARPSLVVRDGR
                     SMRFQDMGRLAAGDNVYIFVPDRYPRLLDKLFASRAIVDAEDADFFGAFAVDPARPAA
                     ELDAAYEIGLNEAERKLTVAALLVERLGSAAEYADRVPIGPIELIVRDVDEKGQITGI
                     GLSFEPSAPVARVPVFLSGGEIADRIVALVRRWKNRPAAKRDAPSPDAATPAASAPVS
                     EAANVAKAEEDAPQTV"
     gene            complement(3201..4211)
                     /gene="gap"
                     /locus_tag="VGM46_05275"
     CDS             complement(3201..4211)
                     /gene="gap"
                     /locus_tag="VGM46_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEY4192034.1"
                     /translation="MTVRVAINGFGRIGRNILRAIYESGRKDIEVVAINDLGPVETNA
                     HLLRYDSVHGRFPHEVKVEGDEIAIGGSRFKVCAVRDPAQLPWKELGVDIALECTGIF
                     TSKDKASAHLTAGAKRVLISAPADGADLTVVYGVNHHKLTKDHVVVSNASCTTNCLAP
                     VAMVLNDAFGIEKGFMTTIHSYTGDQPTLDTMHKDLYRARAAAMSMIPTSTGAAKAVG
                     LVLPELKGKLDGVSIRVPTPNVSVIDFKFVPKRNVTVDEVNAVLVAAASGPLKGILAY
                     TQEPLVSIDMNHNPASSTFALDQTKVVEGNLVRVMSWYDNEWGFSNRMLDTAVAFGKT
                     IA"
     gene            complement(4317..6311)
                     /gene="tkt"
                     /locus_tag="VGM46_05280"
     CDS             complement(4317..6311)
                     /gene="tkt"
                     /locus_tag="VGM46_05280"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEY4192035.1"
                     /translation="MTSREEHDRMANAIRFLSMDAVEKANSGHPGLPMGCADIATVLF
                     TRFLKYDAKNPHWPDRDRFILSAGHGSMLLYSLMYLTGYEDITLDQIKNFRQLGSRTA
                     GHPEYGHAAGIETTTGPLGQGLANSVGFALGERIMNAAFGKDLVDHYTYVLAGDGCLM
                     EGISQEALTLAGHLKLNKLIVFWDNNDISIDGAVSLADSTDQVARFQASGWNAMHVDG
                     LDPEAIAYAIEAARHSDKPTLIACKTTIGFGAPTKAGTNKAHGSPLGAEDIAGARKFF
                     NWDSKPFDIPADILEAWRAAGQAGVKARTEWEGRLAKADAAKRAEFERRISGKLPQNF
                     DAVITDYKKKLAADKPKVATRKSSEMALEVINGAVPETIGGSADLTGSNNTKTSQTQN
                     ITPLDYGQRYVHYGVREHGMAAALNGLTLHGGIIAYGGTFLCFSDYARPAMRLASLMG
                     IRSIFVMTHDSIGLGEDGPTHQPVEHLAALRAIPNHNVFRPADATETAECWQVAIESE
                     KTPSTLALTRQNLPALRTEFVAENLCAKGAYELAAAEGGEAAVTIFASGSEVEIALGA
                     RTLLQGHGHPTRVVSVPCFELFDKQSDAYRAKTIGKAGVKVAIEAGIRQGWDHLIGSD
                     GIFVGMTGFGASGTIEQLYPHFGITAEAAAKAAEARLHAG"
     gene            6564..6836
                     /locus_tag="VGM46_05285"
     CDS             6564..6836
                     /locus_tag="VGM46_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002964813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4164 domain-containing protein"
                     /protein_id="HEY4192036.1"
                     /translation="MTGETTLKEVIARLGKAIDGLENAVAARLEHERDYSEAEAEVQR
                     MNADRSRLAQELDNSEARAERLEDANKEVSRRLVTAMETIRAVLDR"
     gene            6852..7217
                     /locus_tag="VGM46_05290"
     CDS             6852..7217
                     /locus_tag="VGM46_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018429868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEY4192037.1"
                     /translation="MAQVTVSIDGKQYRMACDEGQEDHLTDLATRFDRYVSHLKDSFG
                     EIGDQRLTVMAGIMVMDELSELQKRVKGMESEVQTLRKTRDDALTKADKNDSALTGAL
                     GSLAVRMEDLAATLAVKKG"
     gene            7408..8067
                     /locus_tag="VGM46_05295"
     CDS             7408..8067
                     /locus_tag="VGM46_05295"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004672227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY4192038.1"
                     /translation="MSLRINDTAPDFTAETTQGKIHFYDWIGDGYAVLFSHPKDFTPI
                     CTTELGAMAGLEGEFKKRNAKIIGISVDPVESHNRWKDDIKTATGHTVNYPMIGDGEL
                     KVAKLYDMLPAAAGESSEGRTPADNQTVRSVFVIGPDKKIKLILTYPMSTGRNFQEIL
                     RAIDSIQLTAKHAVSTPADWKQGEDVVIGGGISDEEAVKRFGSFEKVLPYLRKTKQPA
                     S"
     gene            8183..9055
                     /locus_tag="VGM46_05300"
     CDS             8183..9055
                     /locus_tag="VGM46_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011505013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY4192039.1"
                     /translation="MSAPSARPRVTGFYDRPTGSIQYVVADPATGRCAIIDPVLDFDE
                     KSGATATVNADALLEFTDKSGLEIEWILDTHPHADHLSAAHYLAKKTGAPTAIGVKIT
                     EVQNLWKTIYNWPDFRADGSQWDRLFAEGETFSIGDIPVKVLFSPGHTLASITYVAGD
                     AAFIHDTLFMPDSGTARTDFPGGSAARLWRSIDEILALPDETRLFTGHDYMPGGRDPV
                     WQSTVAEQRATNSHISKCRTEAEFVAMRETRDATLPMPRLMLHALQVNINGGRLPEPE
                     ANGQRYLKFPLNAL"
     gene            complement(9119..10087)
                     /locus_tag="VGM46_05305"
     CDS             complement(9119..10087)
                     /locus_tag="VGM46_05305"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEY4192040.1"
                     /translation="MSVNVAVFVGSLRKASLTRKVAKAAIDLSSANLRCRLIEIGDIA
                     MYNEDLDAKPPAPWERLRRDIAEADAILFLTPEYNRSIPACLKNVLDVGSRPAGKSVW
                     AGKPAGIISVTPYKLGAFGANHALRQALVFLDVPAMQQPEAYIGDAEKLFDDEGNLTN
                     GNTREFLGKFLTAFESWVSALRADVHGFEDFMKLREKIAAAYSNGDAKPLDGIVANEG
                     AATFFPPSGGTVSGAREVAKRYDGDAKSFSAGSLTKLEALQVQSSGNLAFWTGFQDFD
                     GKIGGEHAAMKLRITEVFRHDGETWRLIHRHADPLAKAARKAEGSK"
     gene            complement(10379..>11225)
                     /gene="rpoH"
                     /locus_tag="VGM46_05310"
     CDS             complement(10379..>11225)
                     /gene="rpoH"
                     /locus_tag="VGM46_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006200777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEY4192041.1"
                     /translation="RRFPMLQPQEEYMLGKRYAEHEDTSAAHRLVTSHLRLVAKIAMG
                     YRGYGLPIGEVISEGNVGLMQAVKKFEPERGFRLATYAMWWIKASIQEYILRSWSLVK
                     MGTTANQKRLFFNLRKVKGKIQALDDGDLKPEQITEIATRLNVSEAEVVSMNRRLSGD
                     ASLNAPIRASEGEAGEWQDWLVDDHDSQEQMLIEQDELENRRGMLTGALAVLNDREKR
                     IFEARRLSDEPLTLEELSSEFDISRERVRQIEVRAFEKVQDAVKAAAKRQNQALRTIE
                     AQPAA"
BASE COUNT         2189 a         3517 c         3500 g         2019 t
ORIGIN      
        1 gccgatgtcg aacggttcga tttcgaaggc gccaaacggc ccattccaga ccagcgtctt
       61 gcagcgcgca agcaccgaca cgacgtgctc gacgctgcgt ggcccgatgt cgagcatcat
      121 gtcggtcgcg gcaatttcct caatcggcac cacgcgagtg gggacatggg ccgcgagctt
      181 ctgcgccacc accgcgtcca ccggcagcac gatttcgcgc ttgagcgact tggccttggc
      241 gaggatatcg cgcgcgttcg ggatcagatc atgttcgcac agcgatttac cgatcggctt
      301 gccctgggcg gcgagaaacg tgttggccat gccgccgccg atgaccagcg cgtcgacctt
      361 gcgcaccaga ttcatcagca ggtcgatctt ggtcgatact ttcgcgccgc cgacaagcgc
      421 caccaccggc ttggccgggt tgccgaggcc ctgttccagc gcgtcgagtt cggcctgcat
      481 ggtgcgcccg gcaaaggccg gcagccgatg cgccagccct tccgtcgagg catgcgcgcg
      541 atgcgccgcc gaaaacgcgt cgttgacata gatgtcgccg tttttcgcca gtgcgtcggc
      601 gaaatccggt tcattcttct cctcgccctt gtggaaccgc gtattttcca ggagcaggat
      661 atcgccgtcc ttcatcgcgc cgaccgcgtt ggcggcttcc gcgccgatgc agtcggcggc
      721 aaaggcgacc ggcttgccga tcatcgctga gacggcgtag gcaatcgggt cgagcgagaa
      781 ttcgggcgcc ggcccgcctt tcgggcggcc gaaatgggcc agcaggatga ccttggcgcc
      841 ttttttcgac aattcgtcga gcgtcggaag aatgcgctcg atgcgggtcg tgtcgctgac
      901 cttgccatca gtcactggca cgttgagatc gacacgcacc agaacgcgct tgcccgcaat
      961 attgccgata tcgtcgagtg tcttgaagct ggccatacca ttcccttccg aaaaacctgc
     1021 cggaccttac cgggcgtttt gtgcgatgca agacctgtgg ggcgcagaaa cccgcgcggc
     1081 cggttcagac agtctgcggc gcgtcttctt ccgcttttgc cacgttcgcc gcctcggata
     1141 cgggcgcgct tgccgccggc gtggccgcgt cgggcgatgg cgcgtcccgc ttcgccgccg
     1201 gccggttctt ccaacggcga acaagggcga cgatccggtc ggcgatttcg ccgccgctga
     1261 ggaagaccgg cacccgcgcc accggcgcgc tcggctcgaa cgacaggccg atcccggtaa
     1321 tctgcccctt ttcgtcgaca tcgcgcacga tcagctcgat cgggccgatc ggcacgcggt
     1381 ccgcatattc ggcagcactg ccaagccgct cgaccagcaa cgccgcgacg gtcagcttgc
     1441 gctccgcctc gttcaagccg atctcgtaag cggcatcgag ttcggccgcc gggcgggccg
     1501 gatcgacggc gaaggcgccg aagaaatcgg cgtcttcggc atcgacgatg gcgcggctgg
     1561 cgaacagctt gtcgagcagg cgcggatagc ggtcgggcac gaaaatgtag acattgtcgc
     1621 cggcggccag ccggcccatg tcctgaaagc gcatcgaacg cccgtcgcgc accaccaggg
     1681 aaggcctggc ccagcgcgga atgcgctcgc cccgcgccac cggagagccg gccgccacct
     1741 tgtaggccag caattcatgg cgcgccgaac ccggcaattc aagctcgacc ttgtcgagcg
     1801 ggccaaggcg cgccggcacg atgagaccaa gccggcgcgc cagtgggccg accgtccagc
     1861 cctgaatgac cagcgacacc aatacgatga tgaaagcgac gttgaagatg agccgcccat
     1921 gctccagccc gctaagcaac ggcgtgatgg caagcaggat ggaaaccgcg ccgcgcaggc
     1981 ccacccagga aatgaacgcc acttccggcc gtggcagccg gaaaggcatc aggcaaagcc
     2041 aaacggcaag cggccgggcg acgaatatca ggaacaggcc gagcaacatc gccggcagca
     2101 gcaattcgcc aaattgcgac ggcgtcgcga acaggccgag gatgaggaac atgatgatct
     2161 gcgccagcca tgacatgccg tcctggaacc gtttcagcac ggcgacggcg cgaatgtcgg
     2221 aattgccggc aaccagccct gccaggtaga cagcgaggaa ccccgacccg ccgatcgtcc
     2281 cggcggcggc gaacaccatc agcgacaatg tcagcacgaa gatcggcagc aagccatggt
     2341 cgaggttgag ccggtcgacg aggcgcacga tggccatgcc gccgagaacg ccgacgatgg
     2401 cgcccaggcc catgttcacg aagaagccta aaatgaggtc ggtaaccagc accttggctt
     2461 ccggatgcgc cccggcggcg atgacttcga ccagggtgat ggtgaggaag atggcaatcg
     2521 gatcgttggt gccggattcg acctccagcg tcgagcgcac gcgctcgcga aggttgatct
     2581 cgccggcgcg cagcaggaag aacaccgccg ccgcgtcggt cgaggcaacg gcggccccca
     2641 gcaggaagga ttcaagccag gtaaggttga gcagatagaa aacggcgacg ccgaacacgc
     2701 cggtggtgat gagtacgccg accgtcgcca gcgacagggc cggcaacgcg gcctgcttca
     2761 gcgcccgcaa cggcgtgccg aaaccggaat cgaacaggat gacggcgagc gccagcgagc
     2821 cggcgaaata ggcgacgcgc gcattgtcga actggatgcc gaggccatcg gcgccgctga
     2881 cgagaccgat gccgaggaac aaaagtagca gcggggcgcc gaagcgaaag gcgatcaggc
     2941 tcgaaaacgc ggcggccacc acaaggccgg tgccgaccag cgtcacgaga tagatcgcat
     3001 gctccatctt ccgtcgtctc cccttcgcgc ccgttgcccg ccaaccagcc tgtttcgtgc
     3061 ttaagacaca gtgcggcgaa gacaaggcga tacgcaaatc ttttgagcct gaagccgaac
     3121 aggcgggctt tttaatgcgc agcccgaaac gaaaacgccc ggccaaggcc gggcgttcga
     3181 ggtctaaaag cttgaagcaa tcaggcgatg gtcttgccga acgccaccgc tgtatccagc
     3241 atgcggttcg agaaacccca ttcattatcg taccacgaca tgacgcgaac cagattgccc
     3301 tcgaccacct tggtctggtc gagcgcgaag gtggaggaag ccgggttatg gttcatgtcg
     3361 atcgaaacca gcggttcctg ggtataggcg agaatgccct tgagcggacc ggatgcggcg
     3421 gcgaccagaa ccgcgttcac ctcgtcgacg gttacattgc gcttcggcac gaacttgaag
     3481 tcgatgaccg agacattcgg cgtcggaacg cggatcgaca cgccgtcgag cttgcccttc
     3541 aattccggca ggacgaggcc gacggccttg gcggcgcccg tcgaggtcgg gatcatcgac
     3601 atggcggcgg cgcgggcgcg gtagagatcc ttgtgcatcg tatcgagcgt cggctggtcg
     3661 cccgtatagg agtggatcgt cgtcatgaag cccttttcga tgccgaaagc gtcgttcaac
     3721 accatggcga ccggcgcaag gcaattggtg gtgcaggacg cgttggaaac gacgacatgg
     3781 tccttggtga gcttgtggtg gttgacgccg tagacgaccg tcaagtcggc cccgtcggca
     3841 ggggctgaaa tcagcacgcg cttggcgccc gccgtcagat gcgcggaggc cttgtccttg
     3901 gaggtgaaaa tgcccgtgca ttcgagcgcg atgtcgacgc cgagttcttt ccagggcaat
     3961 tgggcagggt cgcgcaccgc gcagaccttg aacctggatc cgccgatggc gatctcgtcg
     4021 ccctcgacct tcacctcatg cggaaagcgg ccgtgcacgc tgtcgtagcg cagcagatgc
     4081 gcgttggtct cgaccggacc gagatcgttg atggcaacga cctcgatgtc cttgcgaccg
     4141 gattcgtaga tggcgcgcag gatgttgcgg ccgatgcggc caaagccgtt aatggcaact
     4201 ctgacagtca tttatttctc ctggggcgga agaattccgt cgcttgtggc ggcgcaggcc
     4261 gagagccacc cgccgcggtt tatcaaatcg ttcaacttcg gcacaggtcc gagccgttag
     4321 ccagcgtgca gccgcgcctc agcagctttg gccgcagcct cggcggtgat gccgaaatgc
     4381 ggatagagct gctcgatggt gccggaggct ccgaagccgg tcatgccgac aaaaatgccg
     4441 tccgacccga tcaaatggtc ccagccctga cggatgcccg cttcgatggc gaccttgacc
     4501 cccgccttgc ctatcgtctt ggcgcggtag gcgtcgctct gcttgtcgaa caattcgaag
     4561 cagggcacgg aaacgacacg ggtcgggtgg ccatggcctt gcagcagcgt gcgcgcgcca
     4621 agcgcgatct cgacttcgga acccgaagcg aagatggtga ccgccgcttc gccgccttcc
     4681 gccgccgcca gttcataagc acccttggcg caaaggtttt ccgcgacgaa ttcggtgcgc
     4741 agcgcgggca ggttctggcg ggtcagcgcc agcgtcgaag gcgtcttttc agactcgata
     4801 gccacctgcc agcattccgc cgtttcggtt gcgtcggccg gccggaacac attgtggttg
     4861 ggaatggcgc gcaacgccgc cagatgctcg accggctgat gggtcggtcc atcctcgcca
     4921 aggccgatgg aatcatgcgt catgacgaag atcgagcgga tgcccatcag cgaggcgagc
     4981 cgcatggccg gccgcgcata gtccgagaag cacaggaacg tgccgccata ggcgatgatg
     5041 ccgccatgca gcgtcaggcc gttgagcgcc gccgccatgc catgctcgcg cacgccgtaa
     5101 tggacatagc gctggccgta atcgagcggc gtgatgttct gggtctggct ggtcttggtg
     5161 ttgttggagc cggtaaggtc ggccgagccg ccaatggttt ccggcaccgc gccgttgatg
     5221 acctccagcg ccatttccga ggatttgcgg gtcgccacct tcggcttgtc ggcggccagc
     5281 ttcttcttgt agtcggtgat gacggcgtcg aaattctgcg gcagcttgcc gctgatccgg
     5341 cgctcgaact cggcgcgctt ggccgcatcg gccttggcga ggcggccttc ccattcggtg
     5401 cgcgccttga cgccagcctg gccggcggcg cgccatgctt cgagaatgtc ggctggaata
     5461 tcgaaaggct tcgaatccca gttgaagaac ttgcgcgcgc cggcaatgtc ttccgcgcca
     5521 agcggagagc catgcgcctt gttggtgccg gccttggtcg gcgcgccgaa gccgatcgtc
     5581 gtcttgcagg cgatcagcgt cggcttgtcg gaatggcgcg ccgcctcgat ggcataggcg
     5641 atcgcctcgg gatcaagccc gtcgacatgc atggcgttcc agccggaagc ctggaaccgg
     5701 gcgacctgat cggtgctgtc ggccagcgaa acggcgccgt cgatggagat gtcgttattg
     5761 tcccagaaca cgatcagctt gttcagcttg aggtggccgg ccagcgtgag cgcctcctgg
     5821 ctgatgcctt ccatcaggca gccgtcgccg gccagcacat aggtgtagtg atcgacgaga
     5881 tccttgccga acgccgcgtt catgatgcgc tcgccgagcg cgaacccgac cgaattggca
     5941 aggccctggc cgagcgggcc ggtcgtcgtc tcgatgccgg cggcatggcc gtattcggga
     6001 tggccggcgg tgcgcgaacc caactgacgg aaattcttga tctggtcgag cgtgatgtcc
     6061 tcgtagccgg tgaggtacat cagcgagtag agcagcatcg aaccgtggcc agccgacagg
     6121 atgaagcggt cgcggtcggg ccaatggggg ttcttcgcat cgtatttcag gaagcgggtg
     6181 aaaagcaccg tggcgatgtc ggcgcagccc atcggcaggc cgggatgacc tgaattggcc
     6241 ttctcgacgg catccatgga gagaaaacgg atagcattgg ccatccggtc atgttcttca
     6301 cgcgaggtca tagttcctcc agggcaaggt agggcttggg agcactttga aaggtggggc
     6361 gacacatagc agccataggc tttgagtcaa caaatcggtg cgttttcatg gcctggcgtc
     6421 gccgattggc ccttacttta gcgttagcgg gggaaagccc atagagcttt gttgacgcat
     6481 gcttcacccg gcgcctaatg tttcacggtt aacgcgttct gaacgtgaac gattcggtga
     6541 tgggcaacaa cgggacacag gccatgaccg gcgaaacgac tctcaaggaa gtcattgccc
     6601 ggctgggcaa ggctatcgac ggcctggaaa acgccgtcgc ggcgcggctt gaacacgagc
     6661 gcgactactc ggaagccgag gccgaagtgc agcgcatgaa cgcagaccgc tcgcggctgg
     6721 cgcaggaact cgacaattcc gaagcgcgcg ccgagcggct ggaggacgcc aacaaggaag
     6781 tctcccgcag gctggtgacc gcgatggaaa ccatccgcgc ggtgctggac aggtaggcga
     6841 ggataggtcg gatggcacaa gtcacggttt ccatcgacgg caagcaatat cgcatggcct
     6901 gcgacgaagg gcaggaagat cacctgaccg acctggccac gcgcttcgac cgctatgtct
     6961 cgcatctgaa ggattccttc ggcgagatcg gcgaccagcg gctgaccgtg atggccggca
     7021 tcatggtgat ggacgaactt tccgaactgc aaaagcgcgt caagggcatg gaaagcgagg
     7081 tccagacctt gcgcaagacg cgcgacgacg cgctgaccaa ggccgacaag aacgattccg
     7141 cgctgaccgg cgcgctcggc tcgcttgccg tgcgcatgga agacctggcg gcgacgctgg
     7201 ccgtcaagaa gggctgatct ttcgtcggcc gacgaaactg catgccaata aacgcggtgc
     7261 gccgggttta ttttgctcag gcacggcaat tcatttcgct gtttggtttt gagcggcgcc
     7321 gcgatggtga taaaatgccg gcgcggcaca agcggccgat tccgatttca agacaggccc
     7381 gatttcaagg ataggtagga acacgccatg agccttcgca tcaacgatac ggcccccgac
     7441 ttcaccgccg agacgacgca gggtaaaatc catttctacg actggatcgg cgacggctac
     7501 gcggtgctgt tttcccaccc caaggatttc acgccgatct gcaccaccga actcggcgcg
     7561 atggccgggc tggagggcga gttcaagaaa cgcaatgcca agatcatcgg catctcggtc
     7621 gatcccgtcg aaagccacaa tcgctggaag gacgacatca agacggcgac cggccacacc
     7681 gtcaactatc cgatgatcgg cgacggcgaa ctgaaggtcg ccaaactcta cgacatgctg
     7741 ccggcggccg ccggtgaaag ttcggaaggc cgcacgccag ccgacaacca gacggtgcgt
     7801 tcggtgttcg tcatcggccc cgacaagaag atcaagctga tcctcaccta tccgatgtcc
     7861 acgggccgta atttccagga aatcctgcgc gccatcgatt ccatccagct caccgccaaa
     7921 cacgccgtgt cgacgccggc ggactggaag cagggcgaag atgtcgtcat cggcggcggc
     7981 atttcggacg aggaagccgt caagcgcttc ggctctttcg aaaaagtcct gccctatctg
     8041 cgcaagacga agcaacctgc ctcctgaccc ctaattccgc gcggccaaac ggcgcttgac
     8101 tgcattggcc gcgcgatgaa tatactcccc ttcgaccagc ccggagaata ttattcctga
     8161 gggcggagat tggggaacaa gattgagcgc tccatccgcc cggcctcggg tgaccggttt
     8221 ttacgacagg ccgactggat cgatccagta tgtggtggcc gatcctgcca ccgggcggtg
     8281 cgccatcatc gaccccgttc tcgatttcga cgagaagtcc ggcgcgaccg cgaccgtcaa
     8341 tgccgacgcg ctgctcgaat tcacggacaa gagcgggctt gagatcgagt ggatactcga
     8401 tacccatccg catgccgatc atctttccgc cgcccactat ctcgccaaga agaccggcgc
     8461 gccgaccgcc attggggtga aaatcaccga agtccagaat ttatggaaaa ccatctataa
     8521 ttggccggat ttccgcgccg acggctcgca atgggacagg ctgtttgccg agggcgagac
     8581 gttcagcatc ggcgacatac cggtgaaagt ccttttctcg cccggccata cgcttgcctc
     8641 gatcacctat gtcgccggcg acgccgcctt catccacgac accctgttca tgcccgacag
     8701 cggcacggcg cgcacggatt ttcccggcgg aagtgcggcg cggctttggc gttcgatcga
     8761 cgaaatcctg gcgctgccag acgagacgcg acttttcacc ggccacgact acatgccggg
     8821 cggccgcgat cccgtatggc aaagcaccgt cgccgagcag cgggcgacga acagccatat
     8881 ttcgaaatgc cgaaccgagg ccgagttcgt cgccatgcgc gaaacccgcg acgccacctt
     8941 gccgatgccg cgcctcatgc tccacgcctt gcaggtcaac atcaatggcg gccgcctgcc
     9001 cgagccggaa gccaacggcc agcgctatct gaaattcccg ctgaatgccc tgtaagcggt
     9061 gacgcggtct gatatcccaa aaggacgcgc cgccgatccg aactcaccaa tatggacttt
     9121 actttgagcc ttcggctttc cgggctgcct tggccaacgg gtcggcgtgc ctgtggatca
     9181 gcctccacgt ctcgccatcg tgccggaaca cttcggtaat gcgcagtttc attgccgcat
     9241 gctcaccgcc gatcttgcca tcgaaatcct gaaaccccgt ccagaaggcc agattgccgg
     9301 acgactgcac ttgcagcgcc tcaagctttg tcagactgcc ggccgagaag gatttcgcgt
     9361 cgccgtcata tcgcttggca acttcgcgcg caccggaaac cgttcctcct gaaggcggga
     9421 agaacgttgc cgctccttca ttggcaacaa tgccatcaag tggcttggca tcgccgttgg
     9481 agtaggccgc cgcgattttc tcacgtaact tcatgaaatc ctcgaaacca tgcacgtccg
     9541 cccgaagcgc cgatacccag ctttcgaacg ccgtcaggaa cttgccgaga aattccctgg
     9601 tattgccatt ggtcaggttg ccctcatcgt cgaacagctt ttccgcatcg ccgatatagg
     9661 cttccggttg ttgcatggcc ggcacatcga gaaacaccag tgcctgacgc agcgcatgat
     9721 tggcgccgaa cgcgccaagc ttatagggcg tcacacttat gatcccggcg ggttttcccg
     9781 cccacacgct cttgcctgcg ggacgcgacc cgacatccag aacgtttttg aggcaggccg
     9841 gtatcgagcg gttatattcc ggcgtcagga aaagaatggc gtccgcttcc gcgatgtcac
     9901 gacgcagccg ctcccacggc gccggaggct tggcgtcgag gtcctcatta tacatggcga
     9961 tgtcgccaat ttctatcagc cggcagcgca agttggcgga tgaaagatcg atcgccgcct
    10021 tggcgacctt acgggtaagc gaagccttgc gaagacttcc cacaaaaacg gcgacattga
    10081 cggacatgga tcgctccctt tgacgtttgc attgaaacgg ggagcgcgcc tctatcgttc
    10141 cagtcgaaag cacgatgcag caacctgacc ttgcatggag ggcaagggat tgcgttacct
    10201 gcgccgtgac aaccaagacc gattggaagg ggatgctaag atcgcgcacg ttgcgacggc
    10261 attattcgtg gccgttccat cagggccggg catcgaacgc cccaaacaga aatcgcgccg
    10321 gtttgaccgg cgcgatttgc attttctgaa tgtcttcgaa tatccggcga cgtcggcttc
    10381 aggccgccgg ctgcgcctcg atggtgcgca gcgcctggtt ctggcgcttg gccgcggcct
    10441 tcaccgcgtc ctgcaccttc tcgaaagccc gaacctcgat ttggcgcacg cgctcgcgcg
    10501 aaatgtcgaa ctcgctcgac aattcttcca gtgtcagcgg ctcgtcggac aggcggcgcg
    10561 cttcgaagat gcgcttttcg cggtcgttca gcacggccag cgcaccggtc agcatgccgc
    10621 gccggttctc cagctcgtcc tgctcgatca gcatctgctc ctggctgtcg tggtcgtcga
    10681 ccagccagtc ctgccattcg ccggcctcgc cttcgctggc gcggatcggc gcgttgagcg
    10741 atgcgtcgcc ggacaggcgg cggttcatcg aaaccacctc cgcttcggaa acgttcagcc
    10801 tcgtcgcgat ctccgtgatc tgctctggct tcaggtcgcc gtcgtccagc gcctggatct
    10861 tgcccttcac cttgcgcagg ttgaagaaca gccgcttctg gttggcggtg gtgcccatct
    10921 tgaccaggct ccacgagcgc aggatgtatt cctggatcga ggccttgatc caccacatgg
    10981 cataggtggc caggcggaaa ccgcgctccg gctcgaattt cttgacggcc tgcatcaggc
    11041 cgacattgcc ttccgaaatc acttcgccga tcggcaggcc ataaccgcga tagcccatgg
    11101 cgatcttggc gacgagccga aggtggctgg tgacgagcct gtgcgcggcg gaagtgtctt
    11161 catgctcggc gtaacgcttg ccgagcatat attcttcctg cggctgaagc atcgggaagc
    11221 ggcga
//
LOCUS       DASYCS010000165         4883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2791624,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000165 DASYCS010000000
VERSION     DASYCS010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4883
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2791624"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="VGM46_05315"
     CDS             complement(<1..736)
                     /locus_tag="VGM46_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HEY4192042.1"
                     /translation="MSVSKEIVLERLKTVSGPDFSGNIVELGMVSEIFIADGKVFFSI
                     TVAAARAKEMEPLRAAAERVVKAIPGVTSAVVALTAEKKSGGMEAAVQRPAPRPTPQP
                     APAPTARPAPQAPASHSHGKRGVPGIAAIIAVASGKGGVGKSTTAVNIALGLAANGLK
                     VGVLDADIYGPSMPRLLGIHGRPQTVDGKVLKPMENYGLKVMSMGFLVDEETPMIWRG
                     PMVISALTQMLREVEWGPLDVLVVDMP"
     gene            complement(810..1814)
                     /locus_tag="VGM46_05320"
     CDS             complement(810..1814)
                     /locus_tag="VGM46_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013891381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEY4192043.1"
                     /translation="MEASLAFPLLAGLVAVALVFDFLNGLHDAANSIATIVSTRVLRP
                     QYAVLWAAFFNFIAFMFFGLHVAETLGTGIIDASIVTPAVIFAALVGAIVWNVLTWLV
                     GIPSSSSHALIGGLVGAGVAKAGTGAIVWSGLVKTVSAIVLSPLAGFLLALLLVLAVA
                     WIFVRQTPFAVDRSFRILQFFSASLYSLGHGGNDAQKTMGIIAVLLYSQGMLGDKFYV
                     PLWVVLTCQSALALGTLFGGWRIVHTMGSKITRLNPMQGFCAETGGAITLFAATWLGV
                     PVSTTHTITGAIIGVGAARRVSAVRWGVAKSIVVAWVVTLPATAAISALTYLAVTLAG
                     "
     gene            complement(1815..2459)
                     /locus_tag="VGM46_05325"
     CDS             complement(1815..2459)
                     /locus_tag="VGM46_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003531900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HEY4192044.1"
                     /translation="MLNWFRKLLPREDRFFDLFERHSRTVVGGAEALQHLLAGGPDVE
                     RWGQEIIDLEDQADAIAAEVLLAVRRSFITPFDRGDIKDLIQSMDDAIDMMRKTVKTV
                     RLFEKTEFDPLMQEMGKTIVEAAHLVAEAIPLLGKLGANVPRLTAIAEEVTRVEDRSD
                     DLHDQGLKDLFRRHARSDPMAYMVGSEIYGQLEKVVDRFEDVADEISGIVIENV"
     gene            2611..3510
                     /locus_tag="VGM46_05330"
     CDS             2611..3510
                     /locus_tag="VGM46_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010913194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY4192045.1"
                     /translation="MIEKLEYFIALAREKHFGRAAEMCNVTQPTLSAGIKQLEGQLGV
                     MLVKRGSRFQGLTQEGAQVLTWARRIAGDARSMREEMQAAKHGLSGRIRIAAIPTALA
                     MLPRLTTPFREKHPGVTFSILSHTSIEILSLLGNFEIDAGVTYLDNEPLGRVTSVPLY
                     EERYQLITAIGNPYSRNRDVTWSQVSQLPLCLLTPDMQNRRIIDQHLAEAGEQARPTL
                     ESNSMLVLFSHIATGRWSSIMPLNLAETFGFGEPIQAIPIVDPDASHTVGLVAGPREP
                     RTPLVSALLEEAAALANGFRASR"
     gene            3640..4116
                     /locus_tag="VGM46_05335"
     CDS             3640..4116
                     /locus_tag="VGM46_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006325044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HEY4192046.1"
                     /translation="MVQSASTEIASRTMSIIDEMKDLEGPLLPILHGIQHEFGYVPRE
                     ALPVIASGLNLSRAEVHGVATFYHDFRSHPAGRHVLKLCQAESCQSMGSDAVAAKLKQ
                     LLGIDFHETTRDGAVTLEPVYCLGLCACSPAAMLDGEVIGRIDDGRIDEIMAQVRS"
     gene            4113..>4883
                     /locus_tag="VGM46_05340"
     CDS             4113..>4883
                     /locus_tag="VGM46_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014766164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HEY4192047.1"
                     /translation="MIPRIYIPGDSGALALGAEKVAEAIRAELSARGIDAKIVRNGSR
                     GAYFLEPMVEVACDNGRVAYGPVKPSDVKSLFDSGFLSGAHHKRWLGNPDKIPFFAGQ
                     TRLTFARCGVTDPLSLDSYKKHGGLKGLQNAVAMEPAAIVAQVTESGLRGRGGAGFPT
                     GIKWKTVLDAQAERKYIVCNADEGDSGTFADRMIMEGDPFVLIEGMTIAGIATGATKG
                     FIYIRSEYPHAVSIMRRAVEVARRSGVLGVNVLGSAHAF"
BASE COUNT          912 a         1569 c         1562 g          840 t
ORIGIN      
        1 gcggcatgtc gaccaccagc acgtcgagcg gaccccattc gacctcgcgc agcatctgcg
       61 tcagcgctga aatcaccatc gggccacgcc agatcatcgg cgtttcctcg tccaccagga
      121 agcccatcga catgaccttc aggccgtaat tctccatcgg cttcagcact ttgccgtcaa
      181 ccgtctgcgg gcggccatga atgccgagca ggcgcggcat ggaaggaccg taaatgtcgg
      241 cgtcgagcac gccaaccttc aggccgttgg cggcaagacc gagcgcgatg ttgacggcgg
      301 tggtggattt gccgaccccg cccttgcccg atgccaccgc gatgatcgcc gcgatacccg
      361 gcacgccgcg cttgccgtgg ctatgcgagg cgggcgcttg cggcgcgggc cgcgcggtgg
      421 gtgcgggcgc tggttgcggt gtgggcctgg gcgcggggcg ctgcaccgcc gcctccatgc
      481 cgccgctttt cttttccgcc gtcagcgcga caaccgcgct ggtcacgccc ggaatggcct
      541 tgacgacgcg ctcggcggcg gcgcgcagcg gctccatctc cttggcccgc gcggcggcaa
      601 cggtgatcga gaagaagacc ttgccgtcgg cgatgaaaat ctcggaaacc atgccaagtt
      661 caacgatatt gccggaaaaa tccggcccgc tgaccgtttt caggcgttcg agaacgattt
      721 ctttgctgac ggacatgggc tttcctcaat cgtggctgcg gctgagatag tgcaaatccg
      781 cagcgatacc aagcgccagc gcgcagcact cagcctgcca gcgtaaccgc caggtaggtc
      841 agcgcggaaa tcgccgccgt ggccggcagg gtgacgaccc aggcgacgac gatgcttttg
      901 gcgacgcccc agcgcacagc ggagacgcgc cttgccgcgc cgacgccgat gatggcgccg
      961 gtgatggtgt gcgtggtcga gaccggcacg ccgagccagg tggccgcaaa cagcgtgatg
     1021 gcgccgccgg tttcggcgca aaagccctgc atgggattaa gccgggtgat cttggatccc
     1081 atcgtgtgga cgatgcgcca gccgccaaac agcgtgccca gcgccagcgc cgactggcag
     1141 gtcagcacca cccacagcgg cacgtagaat ttgtcgccaa gcatcccctg cgaataaagc
     1201 agcaccgcga tgatgcccat ggtcttctgc gcgtcgttgc cgccatggcc gagcgaatag
     1261 agcgaggcgg aaaagaattg cagaatgcga aagctgcgat cgacggcgaa cggcgtctgg
     1321 cgcacgaata tccaggcaac ggccagcacc agcagcagcg ccagcaggaa accggccagc
     1381 ggcgacaaaa cgatggccga gacggtcttg accagacccg accagacgat ggcgccggtt
     1441 cccgccttgg cgacgccggc gccgaccagc ccgccgatca gcgcatgcga gctgctggag
     1501 ggaatgccga ccagccatgt cagcacattc cagacgatgg cgccgacaag ggcggcgaag
     1561 atgaccgccg gcgtgacgat gctcgcatcg atgatgcccg tgcccagtgt ttccgccaca
     1621 tgcaggccga aaaacatgaa ggcgatgaag ttgaagaagg ccgcccacag caccgcatat
     1681 tgcggacgca gcacgcgggt ggaaacgatg gtggcgatgg agttggcggc gtcgtgcagg
     1741 ccgttcagga aatcgaacac cagcgcgacg gcgacgaggc cggccagcag cggaaaggca
     1801 agcgaggctt ccacctagac gttctcgata acgatgccgc tgatttcgtc agcgacgtcc
     1861 tcaaagcggt cgaccacctt ttcaagctgg ccgtaaattt cgctgccgac catataggcc
     1921 atcgggtcgc tgcgcgcgtg gcggcggaaa agatccttca gcccctggtc gtgcaggtcg
     1981 tcggagcggt cctcgacgcg ggtgacttcc tcggcaatcg cggtgaggcg cggaacgttg
     2041 gcgccaagct tgcccagcag cgggatcgcc tccgccacca gatgggcggc ctcgacgatg
     2101 gtcttgccca tttcctgcat cagcggatcg aattccgtct tttcgaacag ccgcaccgtc
     2161 ttgacggtct tgcgcatcat gtcgatggcg tcatccatcg actggatcag atccttgatg
     2221 tcgccacggt cgaacggggt gatgaaggag cggcgcaccg ccagcagcac ctcggcggca
     2281 atggcgtcgg cctggtcttc gagatcgatg atttcctggc cccagcgttc gacatccggg
     2341 ccgccggcca gcaaatgctg caacgcttcg gcgccgccga ccaccgtgcg ggaatgccgc
     2401 tcgaacaggt cgaagaagcg atcttcgcgc ggcaaaagtt tgcggaacca gttcagcatg
     2461 ttcgacctcg gtagctggct atgcttttcc ttagccccgc gatccggcat ggggaacagg
     2521 caatctcata aaagatgtga tctgtgcgcg gcagcgccgc ttgcggcggc gggaaaattc
     2581 gcccagcttg gcgtaaaaga gataccggaa atgatcgaaa agctcgaata tttcatagcg
     2641 ctggcccgcg aaaaacattt cggacgcgcc gccgagatgt gcaacgtgac gcagccgacg
     2701 ctttcggccg gcatcaaaca gctggaaggc cagctcggcg tcatgctggt caagcgcggc
     2761 tcgcggttcc agggactgac ccaggagggc gcgcaagtgc tgacctgggc gcgccgcatc
     2821 gccggcgacg cccgctccat gcgcgaggaa atgcaggcgg cgaaacacgg cctgtcgggc
     2881 cgcatccgca ttgccgccat cccgacggcg ctggcgatgc tgccccggct gacgacgccg
     2941 tttcgcgaaa agcatccggg cgtgacgttt tcgatcctgt cgcatacttc gatcgagatt
     3001 ctttcgctgc tcggcaattt cgagatcgac gccggcgtca cctatctcga caacgagccg
     3061 cttggccgtg tcaccagcgt gccgctctac gaggagcgct accagttgat caccgcgatt
     3121 ggaaatccct attcccgcaa ccgcgacgtc acctggtcgc aggtcagcca gctgccgctg
     3181 tgcctgctga cgcccgacat gcagaaccgc cgcatcatcg accagcacct ggccgaggcc
     3241 ggcgagcagg cgcggccgac gctggagtcc aactcgatgc tggtgctgtt ttcacacata
     3301 gcgacaggca gatggtcgtc gatcatgccg ctcaacctcg cggaaacgtt cggtttcggc
     3361 gaacccatcc aggcgatccc catcgtcgat ccggacgcca gccatacggt cgggctggtg
     3421 gcggggccgc gcgagccgcg cacgccgctg gtgtcggcgc tgctggaaga ggcggccgcg
     3481 ctggccaatg gttttcgggc ctcgcgctga gcaaagggct gatccggcaa cgatagacat
     3541 cttctatcga tcaacgagac agccttattg atttatcgta tttcctctgg ctttataacg
     3601 acttgggttg attgtttctg ccagggaggg cgctgcataa tggtgcagtc cgcaagtacc
     3661 gaaatcgcat cgcgcacgat gtccatcatc gatgagatga aggatttgga agggccgctg
     3721 ctgcccatcc tgcacggcat ccagcacgaa ttcggctatg tcccgcgcga ggctcttccc
     3781 gtcatcgctt ccggcctcaa cctttcgcgt gccgaggttc acggcgtcgc cacgttctat
     3841 cacgattttc gcagccatcc ggccggccgg catgtgctga agctttgcca ggcggaatcc
     3901 tgccagtcga tgggcagcga cgcggttgcc gccaaattga agcagctgct cggcatcgat
     3961 ttccacgaaa cgacgcgtga cggcgcggta acgctggagc cggtctattg cctggggctt
     4021 tgcgcctgct cgccggccgc catgctggat ggcgaggtga tcgggcggat cgatgacggc
     4081 cgcatcgacg agatcatggc gcaggtgcgg tcatgatccc gcgcatctac attcccggcg
     4141 attctggcgc gctggcgctc ggcgccgaaa aggtcgccga ggcgatccgc gccgagcttt
     4201 ccgcgcgcgg catcgatgcc aagatcgtgc gcaacggttc gcgcggcgcc tacttcctcg
     4261 aaccgatggt cgaggtggcc tgcgacaatg gccgcgtcgc ctacgggccg gtcaagccat
     4321 ccgacgtgaa aagcctgttc gacagcgggt ttctgtcggg cgcgcatcac aagcgctggc
     4381 tgggcaaccc cgacaagatc ccgttctttg ccggccagac gcggctgacc ttcgcccgct
     4441 gcggcgttac cgatccgctg tcgctcgaca gctacaagaa gcatggcggg cttaaggggc
     4501 tgcaaaacgc cgtcgccatg gaacccgcgg ccatcgtcgc gcaagtgacg gaatccggcc
     4561 tgcgcggacg cggcggcgcg ggcttcccga ccggcatcaa gtggaagacg gtgctcgatg
     4621 cgcaggcgga gcgcaaatac atcgtgtgca acgccgacga aggcgattcc ggcaccttcg
     4681 ccgaccgcat gatcatggag ggcgacccct tcgtgctgat cgagggcatg acgattgccg
     4741 gcatcgccac cggcgcgacc aagggcttca tctacattcg ctccgaatat ccgcatgcgg
     4801 tgtccatcat gcgccgggcg gtcgaagtgg cgcgccgctc gggcgtgctc ggcgtcaatg
     4861 ttctggggtc cgcccacgcc ttc
//
LOCUS       DASYCS010000166         4217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2646089,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000166 DASYCS010000000
VERSION     DASYCS010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2646089"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(245..1519)
                     /gene="clpX"
                     /locus_tag="VGM46_05345"
     CDS             complement(245..1519)
                     /gene="clpX"
                     /locus_tag="VGM46_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEY4192048.1"
                     /translation="MSKVSNGGGDSKNTLYCSFCGKSQHEVRKLIAGPTVFICDECVE
                     LCMDIIREENKTSMVKSREGVPTPQEILKVLDDYVIGQPYAKRVLSVAVHNHYKRLAH
                     AGKNNDVELAKSNILLIGPTGSGKTLLAQTLARIIDVPFTMADATTLTEAGYVGEDVE
                     NIILKLLQSADYNVERAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKIMEG
                     TVASVPPQGGRKHPQQEFLQVDTANILFICGGAFAGLDKIISDRGRKTSIGFGATVAS
                     PEDRRSGEVFRLVEPEDLLKFGLIPEFVGRLPVLATLEDLDEPALIQILTEPKNALVK
                     QYQRLFEMENVDLTFHENALSGIAKRAIERKTGARGLRSIMEAILLDTMFELPALEGV
                     REVVISEEVVTGSARPLYIYSDSKEKKGNVSA"
     gene            complement(1830..2459)
                     /locus_tag="VGM46_05350"
     CDS             complement(1830..2459)
                     /locus_tag="VGM46_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEY4192049.1"
                     /translation="MKDPIETYMNLVPMVVEQTNRGERAYDIFSRLLKERIIFITGPI
                     EDGMASLVCAQLLFLEAENPKKEISLYINSPGGVVTSGLAMYDTMQFIKPPVSTLCVG
                     QAMSAGSLLLTAGAKGMRFSTPNAQIMVHQPSGGYQGQATDIMIHAQFTERLKRRLNE
                     IYVKHTGQDYETIHNAMERDNFLTTEQALELGLIDKVLTARDEIEGATA"
     gene            complement(2622..3728)
                     /locus_tag="VGM46_05355"
     CDS             complement(2622..3728)
                     /locus_tag="VGM46_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019857669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY4192050.1"
                     /translation="MNVIVVGAGIDGLATAWSLTKAGHNVTLVEQGAKIPNPLAASGD
                     HHRIIRRAYGNAAGYGRLITEAYEAWDEMWADLGESHLDPRGFICISREPGDEAEEYR
                     DGLVAGGFPLEMIEPDEAVKRWPFLEAGTFNYAFFSPEGGALHCRKIAAGLVKWLRAN
                     GANIYVDSKVVEIDSDAGRVTLAGGETMQADRIVVAAGAWVLKLFPELDGELKTYRTA
                     LAYVEPPADLKAAWQAAPVILDVGGATDGYMIPPTGGAGMKFGSGLHKVPTSDADWNR
                     EPVEGEGEAIRNLFSPPIARIDEYRITEVVTCAYTFTPDEKFLAHEKGRCLVVSACSG
                     HGYKFGAAVGRRVAKAIGDGDIAGLKDWLRAEAA"
     gene            complement(3866..4213)
                     /locus_tag="VGM46_05360"
     CDS             complement(3866..4213)
                     /locus_tag="VGM46_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY4192051.1"
                     /translation="MTEPKFVTIDTGAAAPEEGAPAPDRLISGSPKFRTWNVEERDGL
                     YAGIWEATPGKWRIEYEEWEFCHILSGVSILAEEGGETRTVQAGHSFVLRPGFKGSWE
                     VVETTRKEYVVKL"
BASE COUNT          766 a         1333 c         1271 g          847 t
ORIGIN      
        1 acctttctaa tcgtggtcgc cataaggcca accgcgaatc tcatattaac gaccgaggcc
       61 cgttccgcct attacccatt tggtcgggag tggagagcac ggaatgaggg ccattggcct
      121 tctaccaata gttggaggtg aagccggcaa agatcaaggg ttgatatgcg cggccctgcc
      181 acatcccgat accaaaacgg cgccctgcgg cgccgtccgg tcttctattc atcctaaagg
      241 cgtgtcatgc gctgacattg cccttctttt ccttgctgtc ggagtagatg tagagcggcc
      301 tggcgctgcc cgtcacgact tcctccgaga tcaccacctc gcgcacgcct tccagcgccg
      361 gcagttcgaa catggtatcg agcaggatcg cttccatgat ggagcgcagg ccgcgcgcgc
      421 cggtcttgcg ctcgatggcg cgcttggcga tgcccgacag cgcgttctcg tggaaagtca
      481 ggtcgacatt ctccatctcg aacagacgct gatactgctt gaccagcgcg tttttcggct
      541 cggtcaatat ctggatcagc gcaggttcgt ccaaatcctc cagcgtggcc agcaccggca
      601 gtcggccgac gaactccggg atcaggccga atttcagcaa atcctccggc tcgaccaggc
      661 ggaacacttc gccgctgcgg cgatcctcgg gcgacgccac ggtggcgccg aagccgatcg
      721 aggtcttgcg gccacggtcc gagatgatct tgtccagccc ggcaaaagcg ccgccgcaga
      781 tgaacaggat gttggcggtg tcgacctgca agaattcctg ctgcgggtgc ttcctgccgc
      841 cctgcggcgg cacggaggcg acggtgcctt ccatgatctt caaaagcgcc tgctgcacgc
      901 cctcgcccga cacgtcgcgg gtgatggacg gattgtccga cttgcgcgaa atcttgtcga
      961 tctcatcgat gtagacgatg ccgcgctggg cgcgctcgac attgtagtcg gcggactgca
     1021 acagcttcag gatgatgttt tcgacatcct cgccgacata gccggcctcg gtcagcgtcg
     1081 tggcgtcggc catggtgaaa ggcacgtcga tgatgcgggc cagcgtctgc gccagcaggg
     1141 tcttgccgga gccggtcggg ccgatcagca ggatgttcga cttcgccagt tccacgtcgt
     1201 tgtttttgcc ggcatgggcc aggcgcttgt aatggttgtg gacggcgacc gacagcacgc
     1261 gcttggcgta aggctggccg atcacgtaat cgtcgagaac cttcaggatt tcctgaggtg
     1321 tcggcacgcc ttcgcgcgac ttcaccatcg aggtcttgtt ctcctcgcgg atgatgtcca
     1381 tgcacagttc gacgcactcg tcgcagatga acaccgtcgg gcccgcgatc agcttgcgga
     1441 cctcgtgctg gctcttgccg cagaacgagc aatagagcgt gttcttggaa tcaccgccgc
     1501 cgttgctgac cttgctcatt tcccagtcct ttcatggcgc ccgccgatag tccggctgaa
     1561 agggcgtgta ctcaatctgt cgcggaatac gccgctgccg gtccaccggc ccacacaacg
     1621 cattccctac gaaaaacaaa cagaaaacgt ggtgtttgat tcgcggcggc ccagcaacga
     1681 agctaagccg ccgaaactca atagagcctt aacgtaggca atccctgccc ccgagggaac
     1741 agccaaatgt ggccgaaatg ccgcaaatcc tcgcaaaaag cacgaggcaa cgacagcttc
     1801 gcgccatcgt gatggcggcg agacaaaaat caggccgttg cgccttcgat ttcatcgcgt
     1861 gccgtcagca ccttgtcgat aaggccaagc tccagcgcct gctccgtcgt caggaagttg
     1921 tcgcgctcca tcgcattgtg gatcgtctcg taatcctggc cggtgtgctt gacgtaaatc
     1981 tcgttcaggc ggcgcttcag gcgctcggtg aactgcgcgt ggatcatgat gtcggtggcc
     2041 tggccctgat agccgcccga cggctggtga accatgatct gcgcgttcgg cgtcgagaag
     2101 cgcatgccct tggcgccggc cgtcaacaac agcgagccgg cagacatggc ctgcccgacg
     2161 cacagcgtcg ataccggcgg cttgatgaac tgcatggtgt cgtacatggc cagacccgag
     2221 gtcaccacgc cgccaggcga gttgatataa aggctgattt ccttcttcgg gttttccgct
     2281 tcgaggaaca gaagctgcgc gcacaccagc gaagccatgc cgtcctcgat ggggccggtg
     2341 atgaaaatga tgcgctcctt cagcaggcgc gagaaaatgt cataggcgcg ctcgccacgg
     2401 ttggtctgct cgaccaccat cgggacgagg ttcatgtagg tttcgatcgg atctttcatg
     2461 actgtccctt gtttcggtat ggccaccgcg ccggaggcca gaatcgtgat gaatggttgt
     2521 tgcctgtaga taggatgccg gctcatgctt gtgcaagacc gacgggtccg gttggccggt
     2581 taagtcaaat taaggttgcc ggttaagact tcgccgggat ttcaggcggc ctccgcccgc
     2641 agccagtctt tcaggccggc aatgtcgccg tcaccgatcg ccttggccac ccgcctgccg
     2701 accgcggcgc cgaatttgta gccatggccg gagcaggccg agaccaccag gcacctgccc
     2761 ttctcatgcg ccaggaactt ttcatccggc gtgaaggtgt aggcgcaggt gacgacttcc
     2821 gtgatcctgt actcatcgat gcgggcgatg ggcggcgaaa acaggttgcg gatcgcctcg
     2881 ccctcgcctt ctaccggctc gcggttccag tcggcatcgc tggtcggcac cttgtgcagg
     2941 ccggagccga acttcatgcc ggcgccgccg gtgggcggga tcatgtagcc gtcggtggcg
     3001 ccgccgacat cgaggatgac gggcgcagcc tgccatgccg ccttcaggtc cgcaggcggc
     3061 tcgacatagg cgagcgcggt tcgataggtt ttcagttcac cgtccagttc cgggaacagt
     3121 ttcagcaccc aggcgccggc ggccaccacg atgcgatccg cctgcatcgt ctcgccgcct
     3181 gccagcgtca cgcgcccggc gtcgctgtcg atctcgacca ccttgctgtc gacgtagata
     3241 ttcgcgccat tggcgcgcag ccatttcacc agcccggctg cgatcttgcg gcaatgcagt
     3301 gcgccgcctt ccggcgaaaa gaaggcgtag ttgaacgttc cggcctcaag gaagggccag
     3361 cgtttaaccg cctcgtccgg ctcgatcatt tccagcggga agccgccggc caccaacccg
     3421 tcacggtatt cctcggcctc gtcgcccggc tcgcgcgaaa tgcagataaa tccgcgcgga
     3481 tcgagatggc tttcgccaag atcggcccac atctcgtccc acgcctcata ggcttcggtg
     3541 atcaggcggc cataaccggc ggcgttgcca taggcgcgcc ggatgatgcg gtgatggtcg
     3601 ccggaagcgg caagcgggtt gggaattttt gccccctgct cgaccagggt gacgttatgc
     3661 ccggccttgg tcagcgacca tgcggttgcc agcccgtcta ttccggcccc tacgacaatt
     3721 acattcattg cgcgaggact ccatggaggt tgagcgccgg cgagatcggt atcgatactg
     3781 gaacaagcat agagcgcggc gcgctgcggg caaccagggc tactgatcca gcgcggcgca
     3841 tttcatgccg ccaccgcccg cgcgctcaaa gcttgacgac atattccttg cgagtcgttt
     3901 caaccacttc ccagcttccc ttgaagcccg gcctgaggac gaaactgtgg ccggcctgca
     3961 ccgtgcgcgt ttcgccgccc tcctcggcaa ggatcgagac gccggacaga atgtggcaaa
     4021 actcccactc ttcatattcg atccgccatt tgccgggcgt cgcttcccat atgccggcgt
     4081 aaaggccgtc gcgttcctcg acgttccagg tgcggaattt cggactgccc gaaatcaagc
     4141 gatcgggcgc cggcgcgccc tcctcgggtg cggcggcgcc tgtgtcgatg gtcacgaatt
     4201 tcggttcggt cattgaa
//
LOCUS       DASYCS010000167         5820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4241990,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000167 DASYCS010000000
VERSION     DASYCS010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 5820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5820
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4241990"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1140)
                     /locus_tag="VGM46_05365"
     CDS             complement(<1..1140)
                     /locus_tag="VGM46_05365"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEY4192052.1"
                     /translation="MVPMARYVFITGGVVSSLGKGVASAALGALLQARGYRCRLKKLD
                     PYLNVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTGRSANSHDNITTGRIYKNII
                     ERERRGDYLGATVQVIPHVTDEIKNFVLDGNDDFDFVLCEIGGTAGDIEAMPFLEAIR
                     QLGNDLPRNNAVYVHLTLMPYIQAAGELKTKPTQHSVKELRGIGIAPDILLVRADRHI
                     PKEERKKLSLFCNVRESAVIQALDVGHIYDVPMAYHKEGLDSEVLAAFGIEPAPKPRM
                     EPWQGVSHRIHNPEGEVSIAIVGKYTGLKDAYKSLIEALSHGGIANRVKVKLEWIESE
                     IFEKEDPAPYLEKVNGILVPGGFGERGSEGKILAAKFARERKVPYF"
     gene            complement(1278..2426)
                     /locus_tag="VGM46_05370"
     CDS             complement(1278..2426)
                     /locus_tag="VGM46_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEY4192053.1"
                     /translation="MQLRSFIYLGFCAVLATSSAAIAGTPAGTAAPQSVAKTDVVQIA
                     AKSDAAQLRALKKKKKPTDEDLAQIRQLEAKVATAKAEANAKALEARKLAMREAAKAK
                     AAEAREAFLAKKGVKEKAVVAETATAKASDRKKPAILAGLTKPLEPVQVEEEDPVPAE
                     VMNIAVTGNNGELRSEPKPGEPRQQADNGLFAGLFGGAPASPSISLLPETRALDTVLK
                     QREAKKPFKVKPEFVPQEVEFRGYDAGTIVIDTGARRLYLVESSSTARRYAIAVGKEG
                     LQFKGTVAVGDKQEWPRWIPTLDMQKREPQHYARYKDGMPGGGQNPLGARAIYLYDGK
                     TDTHLRIHGTVAPQSIGTSASNGCFRMINEHVMDLYRRVKMGTKVVII"
     gene            complement(2635..3093)
                     /gene="secG"
                     /locus_tag="VGM46_05375"
     CDS             complement(2635..3093)
                     /gene="secG"
                     /locus_tag="VGM46_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEY4192054.1"
                     /translation="METVLIVIHLMVVLALVGVVLLQKSEGGGLDIGGGSGFMTARGA
                     ANTLTRATAILATIFFVTSLALSIIARYGDKPTDILDRVPASQGQTTGKGVLDQLPGS
                     TKPAAPAGTTPAPAVPSGNDATAPATQPATGTAPATNNAAQPAAPQVPNQ"
     gene            complement(3232..3996)
                     /gene="tpiA"
                     /locus_tag="VGM46_05380"
     CDS             complement(3232..3996)
                     /gene="tpiA"
                     /locus_tag="VGM46_05380"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEY4192055.1"
                     /translation="MTPGIRPLVAGNWKMNGTSASLNELRAIGNGFLGGLDAETEALV
                     CVPATLLSRASEILSSSPVHAGGQDCCTQESGAFTGCISAEMLKDAGASHVIVGHSEC
                     RAIQREDDATVHAKASAAWRAGLVAVICIGETDEERRQGLTLDVLARQIAGSVPNTAT
                     PANAVVAYEPIWAIGTGQTPTAMDVAKAHAHIRARLTEKLGAGAAKMRILYGGSVKPS
                     NAVELLGVANVDGALVGGASLKAADFLAIAEAYRAI"
     gene            4151..>5820
                     /locus_tag="VGM46_05385"
     CDS             4151..>5820
                     /locus_tag="VGM46_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019858171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEY4192056.1"
                     /translation="MLGTLRDAAGSWLAKGLLSILVLSFAVWGISGRLAGGLVGHESV
                     ITVGGTSVSVNDYRLAYDRQISAMSQQLGQRLTHEQATAFGIDNQVLAQLVSGALLDE
                     QARKLGLGLSQDRLAELTREDPAFKGPSGQFDRGTFDAVMNQAGMRPEDYLRSRAKVA
                     VRQQIAEAVSDGLKAPKAFLDAVALYRGEDRTVDYLALPRSLVEPIAAPSDSALSTYF
                     EANKKNYAAPEYRKFSYARLEPEDIMDLSSVSDAQVKDDYEKNKARYTTPEQRTVEQL
                     VFKSKEAATAALDSLKAGATFDKLVTAEGKTQADTLLGTVSKDKIADKAVADAAFSLA
                     VNGVSPVVEGSFGPVLLRVTEIKPETVKTQAEVAEQIRKDLALGEASRILLDAHDSYE
                     DARAAGETLDEAAAKLKLKVITVDAIDRTGQTPDGKIVKDLPQSADLVKAVFDAEAGT
                     ENEGLPTDGEGYVFYEVSAVTPARDRTLDEVRQKVVADWTAAEAAKLLDDKAQALEKR
                     LKTGETLDVIASELKLEKQTKRGLKRDADDADLGKDGAAATFGVGEGGA"
BASE COUNT         1111 a         1809 c         1822 g         1078 t
ORIGIN      
        1 gaaatacggc accttgcgct cgcgcgcgaa tttggcggcc aaaatcttgc cctcggaacc
       61 gcgttcgcca aaaccgcccg gcaccagaat gccgttcacc ttttccagat agggcgcggg
      121 atcttccttc tcgaaaatct cactctcgat ccattcgagc ttgaccttga cacggttggc
      181 gatgccgcca tgcgacagtg cctcgatcag tgacttatag gcgtccttga ggccggtata
      241 tttgccgacg atggcaatcg aaacctcgcc ttccgggttg tggatgcgat gcgacacgcc
      301 ctgccacggc tccatgcggg gctttggcgc cggctctatg ccgaacgcgg ccagcacttc
      361 ggaatccagg ccttccttgt gataggccat cggcacatcg tagatgtggc cgacgtccag
      421 cgcctgaatg accgccgatt cccgcacatt gcagaacagc gaaagttttt ttcgctcttc
      481 cttgggaata tggcgatcgg cgcgcacgag caaaatgtcc ggtgcaatgc cgataccgcg
      541 taattccttc acggaatgct gggtcggctt ggtcttcagt tcgcccgccg cctggatata
      601 gggcatcagc gtcaggtgga catagacggc attgttgcgc ggcaaatcat tgccaagctg
      661 gcggatcgcc tcaaggaacg gcatcgcctc gatatcgccg gcggtgccgc cgatttcgca
      721 cagcacgaaa tcgaaatcgt cattgccgtc gaggacgaaa ttcttgatct cgtcggtcac
      781 atgcggaatg acctgaacgg tcgcgccgag ataatcgccg cgccgctcgc gctcgataat
      841 gttcttgtag atgcggccgg tggtaatgtt gtcgtggctg ttggcggaac ggccggtaaa
      901 ccgctcgtaa tggccaaggt caaggtcggt ttcagcaccg tcgtcggtga cgaacacctc
      961 gccatgctga tagggcgaca tggtgccggg atcgacgttg agatagggat cgagcttctt
     1021 gagccggcag cgatagccgc gtgcctgcaa gagcgcccca agggctgctg aagcgacgcc
     1081 ttttccaagt gaggagacca cgccgccggt gatgaaaaca tatcgcgcca tgggaaccat
     1141 cgcttaacgc ggccgaattg attccgccag agaaaaaacc gccgcgaagg gtttttcccg
     1201 gcttgacgcc actatgcgcc acgcaaaaca aaaagggccg gctcaagccg gccctttcgg
     1261 gtatttcctc gatactatca gatgatgacg actttcgtgc ccatcttcac gcgacggtag
     1321 agatccatca cgtgctcgtt gatcatgcgg aagcagccat tggaggcgct ggtgccgatg
     1381 gattgcggcg caacggtgcc atggatgcgc agatgcgtgt ccgtcttgcc gtcatagaga
     1441 tagatggcgc gcgcgccgag cgggttctgt ccgccgccgg gcatgccgtc cttatagcgc
     1501 gcataatgct gcggctcgcg cttctgcatg tcgagcgtgg ggatccagcg cggccattcc
     1561 tgcttgtcgc caaccgcgac cgttcccttg aactgcaagc cttccttgcc gacggcgatg
     1621 gcatagcggc gggccgtcga ggaggattcg accagataga ggcgacgcgc gccggtatcg
     1681 atgacgatgg tgccggcatc atagccacgg aattcgactt cttggggaac gaattcgggc
     1741 ttgaccttga aaggcttctt ggcctcgcgc tgcttcaata ccgtgtccag ggcgcgggtt
     1801 tccggcaaaa gcgagataga cggcgatgct ggcgcgccac cgaacaggcc ggcgaaaagg
     1861 ccgttatccg cctgctggcg cggctcgccc ggcttcggct cgctgcgcaa ctcgccatta
     1921 ttgccggtga cggcgatgtt catgacttcg gccgggaccg gatcttcttc ctcgacctgt
     1981 accggctcaa gcggcttggt caggccggcc agaatcgcgg gcttcttcct gtccgaagcc
     2041 ttggcggtgg ccgtctcggc gacaaccgcc ttttccttga cgcccttctt ggcgaggaat
     2101 gcctcgcggg cctcggcggc cttggccttg gcggcctcgc gcatcgccag cttcctggct
     2161 tccagcgcct tcgcattggc ctcggccttg gcggtggcga ctttcgcttc caactgcctg
     2221 atctgcgcca gatcctcgtc ggtcggcttc ttctttttct tgagggcccg caactgggca
     2281 gcatcgctct ttgcagcgat ctgcacaacg tcggttttcg ctaccgactg cggcgcggcc
     2341 gtgcctgccg gcgtgccggc tattgcggcg gaactcgtgg cgagtaccgc gcaaaaccct
     2401 aaataaatga agctgcgaag ctgcatggcg aagatccgat cattctaagc tagttgccac
     2461 ggcgtcgccc aacgcccccc aaggacgctg gatatgccgc gcgcaaccta taatcgatta
     2521 gcgttgaaac gctcaagccg gatgcaagcc ggcacccggt cgaatcttcg tgatcacgcg
     2581 gcatttgcca tgactgtgtt caaacgaaca cagtcatggc ccaacgattg ccgcttactg
     2641 gttaggaacc tgaggcgccg caggttgggc cgcattgttc gtggccggcg cggttcccgt
     2701 tgccggctgg gtagcgggag cggttgcatc gttaccggac ggcacggcgg gcgcgggcgt
     2761 ggttccagcg ggggcagcgg gtttcgtgct gcccggcagt tggtccagca cgcccttgcc
     2821 cgtggtctgg ccctgcgagg ccggcacgcg gtcgagaatg tcggtcggct tgtcgccgta
     2881 gcgcgcgatt atggagagcg ccagcgaggt gacgaaaaag atcgtcgcca ggatcgcggt
     2941 tgcacgggta agcgtgttgg ccgcgccgcg agcggtcata aagcccgagc cgccgccaat
     3001 gtcgaggccg ccgccttcgg acttctggag cagcaccacg ccgacaaggg cgagcacgac
     3061 cataaggtgg atgacgatca gtacggtttc cattgtttac cctggcaatg cctcgtgcag
     3121 ccgctggcgc ggccgctgat ttggcggctc aatacatcgc tttgaagcca tttccaagcc
     3181 caagcgccga atatgggaag catcggcgca attacaaacg ccgcgccgcc gtcatatggc
     3241 cctgtaggct tccgcgattg ccaggaagtc ggcggccttc aggctggcgc cgccgacgag
     3301 agcgccatcg acattagcga cgccaagcag ttcgaccgca ttggacggct ttaccgagcc
     3361 accatagaga atgcgcatct ttgcagcgcc tgcgcccagc ttttccgtca gccgcgcccg
     3421 gatgtgggcg tgcgccttgg cgacatccat ggcggtcggc gtctggccgg tgccgatcgc
     3481 ccagatcggc tcataggcaa cgaccgcgtt ggcaggggtg gcggtgttcg ggaccgagcc
     3541 ggcgatctgt cgcgcgagca cgtccagcgt aaggccctgc cggcgttcct catccgtctc
     3601 gccgatgcag atcaccgcaa caaggcctgc ccgccatgcg gcggatgcct tggcgtggac
     3661 ggtggcgtcg tcctcgcgct ggatggcccg gcattccgaa tggccgacga tgacgtggct
     3721 ggcgccggca tccttcagca tttccgccga gatgcacccg gtaaaggcgc cgctctcctg
     3781 cgtgcagcaa tcctgcccgc cggcatggac agggctggac gagagaattt cgctggctcg
     3841 cgaaagcaag gtggccggca cgcagaccag cgcctcggtc tcggcatcga gcccgccaag
     3901 aaagccgtta ccaatcgccc tcaactcatt gagggaggcg ctggtgccgt tcattttcca
     3961 gttgccggcg acaagcgggc gaattccagg ggtcatgttg ttggttctcc agatcggccg
     4021 aagcttaccc ctccctatca aaaacaggtc acaaagcaat cgacaggtga gcgtaaggac
     4081 gctattgcgc ttgtttcaga caagacgcat ccccaaaaaa cgcgcgcctg ccccaaaccc
     4141 ggaagtgact atgcttggta cgttaagaga cgcagcggga tcatggctcg ccaagggcct
     4201 gctctcgata ttggtgctca gctttgccgt ctggggcatt tcgggccgcc ttgccggcgg
     4261 gctggtcggg cacgaatcgg tgatcaccgt cggaggcacc agcgtctcgg tcaacgacta
     4321 ccgccttgcc tatgaccgcc agatcagcgc catgtcccag cagctcggcc agcggcttac
     4381 ccacgagcag gcaacggcgt tcggcatcga caaccaggtg ctggctcagc tcgtatcggg
     4441 tgcgctgctg gacgaacagg cgcgcaagct cggccttggc ctttcgcaag accggctggc
     4501 ggaacttacc cgcgaagacc ccgccttcaa gggaccgagc ggtcagttcg atcgtggaac
     4561 cttcgatgcc gtgatgaacc aggccggcat gcgcccggaa gactatctgc gcagccgcgc
     4621 caaggttgcg gtgcgccagc agatcgccga ggccgtttcg gacggactta aagcgccgaa
     4681 ggcctttctc gacgcggtag cgctttatcg cggcgaagac cgcaccgtcg attatctggc
     4741 cctgccgagg tcgctggtgg agccgatcgc ggctccttcc gacagcgccc tttccaccta
     4801 tttcgaagcg aacaagaaga attacgccgc gccggaatac cgcaaattct cctatgccag
     4861 gctcgaaccc gaagacatca tggatttgag cagcgtcagc gacgcgcagg tcaaggacga
     4921 ctacgagaag aacaaggcgc gctatacgac gcccgagcag cgcacggtgg agcagctggt
     4981 gttcaagtcg aaggaggctg cgacggcggc cctcgattcg ctgaaggccg gggccacctt
     5041 cgacaagctc gtcacggccg aaggcaagac acaggccgac acgctgcttg gcaccgtttc
     5101 caaggacaag atcgccgaca aagccgtggc ggatgccgca ttctcgctcg ccgtgaacgg
     5161 cgtcagcccg gttgtcgagg gcagcttcgg cccggtgctg ctgcgcgtga ccgaaatcaa
     5221 gcctgaaacc gtcaagacgc aggccgaggt tgccgagcaa atccgcaagg atctggcact
     5281 gggagaggca agccgcatcc tgctcgacgc acatgattcc tacgaggatg cacgcgccgc
     5341 cggtgagacg ctcgacgagg ccgcagccaa gctaaaactg aaagtcatca ccgtcgacgc
     5401 catcgaccgc accgggcaga cgcctgacgg aaagatcgtc aaagacctgc cgcagtccgc
     5461 cgacctcgtc aaagcggtgt tcgatgcgga agccggtacg gagaacgaag gcctgcccac
     5521 ggatggcgag ggctatgtat tttacgaggt aagcgccgtc acccctgccc gcgaccgcac
     5581 gctggacgaa gttcgccaga aagtcgttgc cgactggacg gcagcggaag cggccaagct
     5641 gctcgacgac aaggcgcagg cgctggaaaa gcggctgaag actggcgaga cgctcgacgt
     5701 catcgccagc gagctgaagc tcgaaaagca gaccaagcgc ggcctgaagc gcgatgccga
     5761 cgatgccgac ctcggcaagg acggcgccgc agcgacgttc ggcgtcggtg aaggcggcgc
//
LOCUS       DASYCS010000168         9840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2501975,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000168 DASYCS010000000
VERSION     DASYCS010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 9840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9840
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2501975"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1410)
                     /locus_tag="VGM46_05390"
     CDS             complement(<1..1410)
                     /locus_tag="VGM46_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007769809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEY4192057.1"
                     /translation="MTAAKGIGGTTPAVLIAGAGLVLIVAVPFAFIILQAVFLNLGSG
                     SFNAPFAHLFATLANSALIGMTFNTVVLGLSVVACSALLAVPLGIFRALFRVPLAPLW
                     DVLLLVPFMIPPYIATLGWIMTLQPRGYLQQLAGFNLAPFLFSLGGMTLIMALNTFPV
                     VYFAVSRTVEAVGARYSDVARVFGSSPARAFWRITLPLATPGLAASLLLVFAMAIEEY
                     GTPAALGRRAHFEVLVSGIDLRISDWPIDLPGAAVISLILVALSFLAFLIQRRILAGR
                     SFETTGGKPQNKDKRPLGRLALPVVALFAIVAFLATGVPLLAVLATAMQKTISGGLVP
                     GNLGFENFSAIFFTDVAGGLRALGISLALGVATALIAGLLGALSSYVVVKTRLRGRRT
                     IDVLTVIPNALPGIVVAVGLILAWNQPALPFSPYNTPLILLLAYCCILLPQPVRYATA
                     ALLQVGDSLEAAARVCGASG"
     gene            complement(1417..2367)
                     /locus_tag="VGM46_05395"
     CDS             complement(1417..2367)
                     /locus_tag="VGM46_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016556614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY4192058.1"
                     /translation="MLKSALAAVVLAAGALSAGSALADVTVYTAGPQGLIDKLSAGFT
                     AQSGVKVNVFQATTGKVMARIEAEASNPAVDVVISASWDTATDFAKRGWLVPYTSPNA
                     ATVPDFLKTDTAVAQGISGLGIAWNTKSGTPRPTEWADLAKPEYKDMVNIPDPAQSGS
                     SFELTAALGGQDDWKLFKDLKADGAIIAGANADALNPVLQGAKAAVFGAVDYIALAAK
                     AKGESIDVIFPASGTVIAPRPAMIMNWSKNQDDAKKFIDYMLSDEGQKIVAKAMLMPA
                     RTDIAADRPLIGDLKLLKYDTAEVYGKRKETLATVADIFK"
     gene            complement(2403..3506)
                     /locus_tag="VGM46_05400"
     CDS             complement(2403..3506)
                     /locus_tag="VGM46_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006472053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4192059.1"
                     /translation="MLSPSQTAQTCAIAVQGLEQRYGQTPVLRGIDLRLERGRTLALL
                     GPSGCGKTTLLRLLAGLLAPSAGSIAIEGETMADAASGTFVPPERRGLGMVFQDYALW
                     PHMSVAENVGFPLEMRGTPKAERQQRIIAALARVDLAGLADRRPADLSGGQQQRVAIA
                     RAIVAEPRIVLFDEPLSNLDRELRESLVGEIAGLVSSLGLTAVYVTHDHGEAFTLADE
                     VAVMGKGEIVQLASPETLVDAPASPAVAEFLKLGAIAGATCRDGAWWLDNTAVRLPYP
                     AAGSLHGAAASGKARVLLGRKAVRLGPRGGDAIEGTVTQSQFRGDSYMLAVSFGDRSQ
                     PVTVQAASDIRIHAGEKIGLTIEPGNLRWFPAE"
     regulatory      3785..3969
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            4018..4815
                     /gene="bluB"
                     /locus_tag="VGM46_05405"
     CDS             4018..4815
                     /gene="bluB"
                     /locus_tag="VGM46_05405"
                     /EC_number="1.13.11.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019169828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032363 - FMN catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5,6-dimethylbenzimidazole synthase"
                     /protein_id="HEY4192060.1"
                     /translation="MPAAASRQFSNGNSFDRAAQAAVYRAIFTRRDVRSHFLPDAIND
                     AVLARLLLAAHHAPSVGYMQPWNFIVIRDDARRRQVRQLFVEARQIEGEQMQGDRHAL
                     YRSLKLEGICETPLNLVVTCDRSRAKGSPLGRWHNPQMDLFSTVCAVQNFWLAARAEG
                     IGVGWVSILDGDALKRLLDIPDHVVPVAYLCVGHVSGFGSKPDLETHGWGKRLPLADL
                     VMSEKFAGTGEDGLKDAVRAMTSTEVLEPAGTHEQEAVAVASTIHPA"
     gene            complement(4859..5587)
                     /locus_tag="VGM46_05410"
     CDS             complement(4859..5587)
                     /locus_tag="VGM46_05410"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY4192061.1"
                     /translation="MNKPRILIVEDEADIRRFVRMALEKEGMSVFETTTAEEARLNAG
                     SRKPDLMIVDLGLPDGDGKALIREVRNWVSAPILVLSAREQETEKVEALDAGADDYLT
                     KPFGVPELLARVRAQLRRSGTISNGSTTSPVVKFGQVAINLATHEVTRANEPVHLTPN
                     EFRLLTALIRGHGKVLTHRQLLMDVWGPGYAERSHYIRIYMAQLRQKLEDDPSQPAHL
                     ITELQVGYRLSGLEIPEGNRTADP"
     gene            complement(5559..8246)
                     /locus_tag="VGM46_05415"
     CDS             complement(5559..8246)
                     /locus_tag="VGM46_05415"
                     /inference="COORDINATES: protein motif:HMM:NF014728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HEY4192062.1"
                     /translation="MKDQRPDPDDLLEAVKGEAHRSKRGQLKIFFGACAGVGKTFAML
                     QAARQLQAEGVAVGIGIVETHGRKDTEELTAGLPELPRKAMSGNGRPTTEFDLDGALA
                     AGYKLLVVDELAHTNVAGSRHAKRWQDVEELLDAGIDVYTALNVQHLDSLNDIIGGII
                     GIRVRETVPDRIFDAATDVVLVDLPPDDLLSRLNAGKVYLPVSIERARQNFFRRGNLI
                     ALRELALRRVADRVNSDVRTYRVSHAIKTVWPTQELLMVCVSADRSQERLVREGARLA
                     QRLQVKWIVVHVDQPYHGDDQQARETLLSIALAAQGAGAEFANVPGQDVAETLLDFAR
                     KRNATKLIIGNPSGRRYMAFRPRLQERIARADPEIGLILLGVDGVRPLVKRKVAGERQ
                     NYAWSLAVATVACGLTTTVAAWLLRVFDLSNIVMLFLMTVVFVAIRLGRLAGAWAALF
                     SVGCFNFFFVEPTLSFTVADTQYIFTFALMLAVALVIGQLAARLRSEARVARAGERRA
                     SALARIARELSSAIKLDQIVSVCQGAITPLFGAKVVLLVPDRKERLLPTQDDGLIDLS
                     VAQWAFDHTQEAGAGTQTLNAAEALYLPLRASMATRGILAILPDGTALPNNPDDRRLL
                     DACCSSVALALERIHFVEVAQDTLVRMEGEKLRNALLSAVSHDLKTPLTAIRGLAETL
                     ERSKDIPEEDRNDIARSIRVQADELKRLVSNLLDLARMQSEGVHLNKEWHSLSEIVGS
                     ALAQSTTLLRPRSIRTSLPADLPLVDVDAALIERVLINLFDNAAKYTPSTSTVAIRAA
                     VSGDTMYMVVEDDGPGLPMADPDTLFEPFVRGKKESSIPGVGLGLALCRSIIAAHGGS
                     IRAEARKPKGTAFEIRLPLGAPPEIESETVNEQAAHPDR"
     gene            complement(8299..>9840)
                     /gene="kdpB"
                     /locus_tag="VGM46_05420"
     CDS             complement(8299..>9840)
                     /gene="kdpB"
                     /locus_tag="VGM46_05420"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007760761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HEY4192063.1"
                     /translation="DFCSVTGGTRVLSDWIIMRVTARPGEAFLDRMIAMVEGAKRGKT
                     PNEIALTILLVALTLIFLLVCVTLLPFSSFSTVVAGGGSVVTITVLIALLVCLIPTTI
                     GALLSAVGIAGMSRMMKANVLATSGRAIEAAGDVDVLLLDKTGTITLGNREAAQFIPA
                     AQVSEQTLADAAQLASLADETPEGRSIVVLAKQKFNLRGRELHGAEAIAFSAHTRMSG
                     INIDGRQIRKGAASAIRAHVESLGGVFPGEIGKTVDEVSRRGSTPLVVADGGKVLGIV
                     ELKDIVKGGIKERFAELRAMGIKTVMITGDNKLTAAAIAAEAGVDDYLAEATPEDKLR
                     LIREYQADGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLL
                     RVVEVGKQMIMTRGALTTFSIANDLAKYFAIIPAAFVTTYPPLAALNIMGLHSPASAI
                     LAAVIFNALIIVVLIPLALKGVKYRAEPAARLLSRNLLIYGLGGIIAPFIGIKLIDIL
                     LTPFF"
BASE COUNT         1819 a         3107 c         3211 g         1703 t
ORIGIN      
        1 cccgctggcg ccgcagaccc gcgccgccgc ctcgaggctg tcgccgacct gcagcagcgc
       61 cgcggtggcg tagcgcaccg gttgcggcag caggatgcag caataggcca gcagcaggat
      121 cagcggggta ttgtagggcg agaacggcag ggccggctgg ttccaggcga ggatcagccc
      181 aaccgccacg acgatgcccg gcagcgcgtt ggggatgacg gtcagcacgt cgatggtccg
      241 cctgccgcgc agtcgcgtct tgaccacgac ataagaggaa agcgcgccga gcaggccggc
      301 gatcagcgcc gtggcgacac cgagcgccag gctgatgcca agcgcgcgaa ggccgcccgc
      361 gacgtcggtg aagaagatgg cagaaaaatt ctcgaagccg agattgccgg gaacaagccc
      421 gccggaaatg gtcttctgca tggcggtggc cagaacggcg agcagcggca cgcccgtcgc
      481 caggaaggcg acgattgcga acagcgccac cacaggcaaa gccagccggc caagcgggcg
      541 cttgtccttg ttttgcggct tgccgccggt cgtttcgaac gagcgcccgg cgagaatccg
      601 ccgctggata agaaaggcga ggaacgacag cgccaccagg atcagcgaga tcaccgccgc
      661 gcccggcagg tcgatcggcc agtcggatat gcgcaaatcg atgccgctga ccagcacctc
      721 gaaatgggcg cgacggccaa gggctgccgg cgtaccatat tcctcgatgg ccatggcgaa
      781 caccagaagc aggctggcgg ccaggcccgg cgtcgccagc ggcaaggtta tgcgccagaa
      841 agcgcgcgcc ggcgaggagc cgaacacccg cgccacgtcg gaataacgcg cgccgaccgc
      901 ctctaccgtg cgcgataccg cgaaatagac gaccggaaag gtgttgagcg ccatgatgag
      961 ggtcatgccg ccgagcgaaa acaggaacgg cgccagattg aagccggcta attgttgcag
     1021 atagccgcgc ggctgcaacg tcatgatcca gcccagcgtg gcgatatagg gcgggatcat
     1081 gaacggcacg agcagcagca cgtcccacag cggcgccagc ggcacccgaa acagcgcccg
     1141 gaagatgccg agcggcaccg ccagcagcgc agagcaggcg acgaccgaga ggccgagcac
     1201 gacggtgttg aacgtcatgc cgatcagcgc cgaattggcc agcgtagcga aaagatgcgc
     1261 gaaaggcgcg ttgaacgaac ccgatccgag gttaaggaat acagcctgaa ggatgatgaa
     1321 ggcaaaggga acggcgacga tcagcacaag ccccgcgccg gcgatcaaca ccgccggcgt
     1381 cgtgccgcca attccctttg ccgctgtcat gatcgcttac ttgaagatgt cggcgacagt
     1441 cgccaacgtc tccttgcgct tgccgtagac ttcggcggtg tcgtatttca gcagcttcag
     1501 atcgccgatg agcggacggt ccgccgcaat gtcggtgcgg gccggcatca gcatggcttt
     1561 cgccacgatc ttctggcctt cgtcggaaag catgtagtcg atgaatttct tggcgtcgtc
     1621 ctggtttttc gaccagttca tgatcatggc cgggcgcggc gcgatgaccg tgccggacgc
     1681 cgggaagatg acgtcgatgc tctcgccctt ggccttggcg gccagcgcaa tgtagtcgac
     1741 agcgccgaac acggcggcct tggcgccctg tagaacgggg ttcagcgcat cggcattggc
     1801 gccggcgatg atcgcgccat cggccttcag atccttgaac agcttccagt cgtcctggcc
     1861 gccaagtgct gcggtgagtt cgaaggaaga gccggactgc gccgggtcgg gaatgttgac
     1921 catgtccttg tattccggct tggcgaggtc cgcccactcg gtcgggcgcg gcgtgccgct
     1981 cttggtgttc caggcaatgc cgaggcccga aatgccctgc gcgaccgcgg tatcggtctt
     2041 caggaagtcc ggaacggtgg cggcgttcgg gctggtatag ggcaccagcc agccgcgctt
     2101 ggcgaaatcg gtcgccgtat cccacgatgc cgagatcacc acgtcgacgg ccgggttcga
     2161 ggcttcggcc tcgatgcgcg ccatgacctt gccggtcgtt gcctggaaga cattgacctt
     2221 gacgccgctc tgcgcggtaa agccggccga cagcttgtcg atcaggccct gcgggccggc
     2281 ggtgtagacg gtgacatcgg cgagggccga gccggcggac agggcgccgg ccgcaaggac
     2341 caccgccgct agggctgatt tcaacatgat tggcatctct ctcttgattg gctggtcatg
     2401 atctactccg ccgggaacca gcgaagattg ccaggctcga tcgtgagccc gattttctca
     2461 cccgcatgga tgcggatatc gctcgccgcc tgtaccgtga cgggttggga gcgatctccg
     2521 aaggaaacgg ccaacatgta gctgtcaccc cgaaactgcg attgcgtgac ggtgccttcg
     2581 atggcgtcgc cgccgcgcgg tccaagccgc accgccttgc ggcctagcag cacccgcgcc
     2641 ttgccggagg ccgcagcgcc atggagcgac cccgccgccg gatagggaag ccgaaccgcc
     2701 gtattgtcga gccaccatgc gccgtcgcgg caggtcgccc cggcaatcgc gccgagcttc
     2761 aggaactccg ccaccgccgg gcttgccggc gcgtcgacca gcgtttccgg cgaggccagc
     2821 tggacgatct cgcccttgcc catcaccgcc acctcgtcgg ccagcgtgaa tgcctcgcca
     2881 tggtcgtggg tgacgtagac cgccgtcagc ccaaggctgg aaacgaggcc ggcgatttcg
     2941 ccgaccagcg attcccgcag ttctcggtcg aggttggaca aaggctcgtc gaacaatacg
     3001 atgcgcggct cggcgacgat ggcgcgggca atcgccacgc gctgctgctg gcctccggaa
     3061 aggtctgccg gccgccggtc cgccagcccg gcaagatcga cgcgcgccag cgccgcgatg
     3121 atccgctgct gccgctcggc cttgggcgtg ccgcgcattt ccagcggaaa gccgacattt
     3181 tcggccacgc tcatatgcgg ccacagcgca taatcctgga agaccatgcc aagcccgcgc
     3241 cgctcgggcg gaacgaacgt ccccgaagcg gcgtccgcca tcgtctcgcc ctcgatggcg
     3301 atcgagcctg cgcttggcgc aagcaggccg gcaagcaaac gcagaagcgt ggtcttgccg
     3361 cagccggaag ggcctaggag cgccagcgtc cgcccccttt ccagtcgcag gtcgatgccg
     3421 cgcagcaccg gcgtttgccc gtagcgttgt tccagcccct gcacggcaat ggcgcaggtc
     3481 tgggccgtct ggctgggtga gagcatggtg tgcgtttcaa tcgggggccg ggcagccggg
     3541 aatgccagcg gccgcgaagc cccacgctcc ggtccgcagg gagcaattaa gacagctata
     3601 tgacagggat attacagttg gcggggttgg cggcccggct tgccgcgcca aatgccgggc
     3661 tggtccatat tcgcttgcag cgagcgccga tgcaggcttt ccggcctgaa atcccacgat
     3721 aacgctgcct tcgcccaggt gaatgcctgc cgtcattatg ctcgtgcgtt ttgcggcgcg
     3781 gcggtttatg acgatggcgc caggtgcccg ccgcaatgcg ggagaatcgg gaacacggtg
     3841 aaaatccgtg gcgtgcccaa cgctgtatga gggactgcgc cggcagacgt cactgttcga
     3901 cagaacggga agacgccgga agcgggatga tctcaagcca gaagaccggc caggcaaaca
     3961 gtgtcatccg cttggtcaag cggcaggcaa ccgtttgaag tcgcaagggg aaacgcgatg
     4021 ccggcagccg cttcacgtca attcagcaat ggcaattcct tcgaccgggc ggcgcaggcc
     4081 gccgtctacc gcgcgatctt cacgcgccgc gacgtgcgca gtcacttcct gcccgacgcc
     4141 atcaatgatg ccgttctggc gcggctgctg cttgccgccc accacgcccc ctcggtcggc
     4201 tatatgcagc cctggaattt catcgtcatt cgcgacgatg cgcggcgccg gcaggtccgt
     4261 cagctgtttg tggaagcgcg gcagatagag ggcgagcaga tgcagggcga ccgccatgcg
     4321 ctctatcgtt cgctgaagct ggaaggaata tgcgagacgc cgctcaatct cgtggtcacc
     4381 tgcgaccgca gccgggcaaa gggatcgccg ctcggacgct ggcacaatcc gcagatggac
     4441 ctgttcagca cggtttgcgc ggtacagaat ttctggctgg cggcgcgcgc tgaagggatc
     4501 ggcgtcggct gggtcagcat ccttgacggc gatgccctca agcgcctgct cgacataccc
     4561 gaccatgtgg tgccggtcgc ttatttgtgc gtcggccatg tatccggctt cgggtcaaag
     4621 cccgatctcg aaacccatgg ctggggcaag cggctgccgc tggcggattt ggtcatgagc
     4681 gaaaaattcg ccgggacagg ggaggacggg ctgaaagacg cggttcgcgc catgacaagc
     4741 acggaagtgc tggagccggc gggaacgcac gagcaggagg cggtggcggt cgcctcaacc
     4801 atccatcccg cttaagaaaa tcaaggatgc agtccatatg ccggtgaccc cgcgccggtc
     4861 acggatcggc agtccggttg ccttcaggaa tttcaagtcc ggatagacgg tagccaacct
     4921 gcaattcggt gatcaggtgg gccggttgcg aagggtcgtc ctcaagcttc tggcgaagct
     4981 gcgccatgta gatgcggata tagtgggaac gctccgcata tcccggtccc cacacatcca
     5041 tgagcagttg gcgatgggta agcactttgc catgcccccg gatgagagcg gtcagcaggc
     5101 gaaattcgtt cggcgtcagg tgcaccggtt cgtttgcccg agtgacttcg tgggtggcaa
     5161 gattgatcgc cacctgtccg aacttcacga ccggcgaggt cgtcgaacca tttgagatgg
     5221 tgccggaacg gcgcaactgc gcccgcacgc gcgccagcag ttcgggaacg ccgaacggct
     5281 tcgtcagata gtcgtcggct ccggcatcca gcgcttcgac cttttcggtt tcctgttcgc
     5341 gggccgagag cacgaggatg ggtgcggaca cccagttgcg gacttcacgg atcaatgcct
     5401 tgccgtcccc gtccggcagg ccgaggtcga cgatcatcaa atccggtttc cggctgccgg
     5461 cgttgagacg ggcttcctcg gccgtcgttg tttcaaagac gctcatgcct tccttttcga
     5521 gcgccatgcg gacgaagcgg cgaatatcgg cttcgtcttc aacgatcagg atgcgcggct
     5581 tgttcattca cggtctcgct ctcgatttcg ggcggggcgc cgagcggcag cctgatttcg
     5641 aaggcagtcc ccttcggttt gcgcgcttcg gcgcgaatgc ttccgccatg agccgcgata
     5701 atgctgcggc atagggcaag gccaaggccg acgcccggaa tggacgattc cttcttgccc
     5761 ctcacgaacg gctcaaaaag agtgtcgggg tccgccatcg gcaggccggg accatcatct
     5821 tcaaccacca tatacatggt atcgcccgac acggcggcgc ggatggcgac ggtgctcgtc
     5881 gacggcgtat atttggcggc gttgtcgaaa agattgatca gaacgcgctc gataagggct
     5941 gcgtcgacat cgacgagggg caggtcggcg ggaaggctgg tgcggatcga tcgaggcctg
     6001 agaagcgttg tcgattgcgc gagcgcgctg ccgacgatct cgctaagcga atgccattcc
     6061 ttgttcagat gcacgccctc gctctgcatc cgcgccaggt ccagcaagtt gctgacgagc
     6121 cgcttcagtt cgtcggcctg gacacggatg gaacgcgcaa tatcgtttct atcctcctct
     6181 ggaatatcct tggagcgctc cagggtttcc gcaaggccac ggattgccgt gagcggcgtt
     6241 ttgaggtcgt gggataccgc cgaaagaagc gcattgcgca gcttttcccc ctccattcga
     6301 acgagcgtat cttgggcaac ttccacgaag tgaatgcgct caagcgcaag ggctaccgac
     6361 gagcagcacg cgtcgagcag gcggcggtcg tcgggattgt tcggcaaagc cgtcccgtcg
     6421 ggcaggatgg ccagaattcc cctcgttgcc atggaagcgc gaagcggaag gtacaacgcc
     6481 tccgccgcat tcagcgtctg tgtgccggcg ccggcctcct gtgtgtggtc gaaggcccat
     6541 tgggcaaccg acagatcgat cagcccatcg tcctgcgtgg gaaggaggcg ctcttttctg
     6601 tcgggcacga gcagaaccac cttggctccg aacaatggcg tgatcgcacc ctggcatacc
     6661 gatacgatct ggtcgagctt gatggcgctc gacagttccc gcgcgatgcg ggccagcgcg
     6721 gatgcgcgcc gctcgcccgc gcgggcaacc cgcgcttcgg atcgcaagcg cgctgcaagc
     6781 tggccgatca ccagcgccac agccagcatg agggcgaatg tgaaaatata ctgggtatcg
     6841 gcgacggtga acgacagcgt cggctcaacg aagaaaaagt tgaaacaccc gacgctgaac
     6901 aatgccgccc acgctccggc cagacgcccg agacgtatgg cgacgaacac caccgtcatc
     6961 aggaacagca tgacgatgtt ggagaggtcg aagacccgaa gcagccatgc cgccacggtg
     7021 gtcgtcaggc cacaggcgac agtcgcgacc gccaacgacc atgcatagtt ctgccgctcg
     7081 ccggcgacct tgcgtttgac caacggtcga acgccgtcaa cgcccagcag gatcagcccg
     7141 atctccgggt ccgcgcgggc gatccgctcc tgaagccgtg gcctgaaggc catatagcgt
     7201 cgacctgacg ggttcccgat gatgagcttc gttgcgttac gcttgcgggc gaagtccaaa
     7261 agcgtctcgg caacgtcctg gcccggaaca ttggcgaact ccgcccccgc gccctgcgcc
     7321 gcgagcgcga tgctcaacag cgtttcacgc gcctgttgat cgtcgccatg atagggctgg
     7381 tcgacgtgga ccacgatcca tttgacctgc aaccgctggg caagccgcgc tccctcgcgc
     7441 accagtcttt cctgcgaacg gtcggcgctg acgcacacca tgagcagttc ctgcgtcggc
     7501 cagaccgttt tgatggcgtg ggagacccgg taggttcgga catcggagtt cacccggtcg
     7561 gcgacccggc gaagcgccag ctcgcgcagg gcgataagat tgccgcggcg gaaaaagttc
     7621 tggcgcgcgc gctcgatcga gacgggcaga taaaccttgc cggcattcag gcgcgacaac
     7681 aggtcgtccg gaggcaaatc gacgagaacg acatccgtcg cggcgtcgaa tatccggtcc
     7741 ggaacggttt ccctgacgcg gatgccgata atgccgccga taatatcgtt gaggctgtcc
     7801 aggtgctgga cgttcagcgc cgtatagacg tcgatccccg catccagcag ttcctcgaca
     7861 tcctgccagc gtttggcatg gcggctgccg gcgacattgg tgtgggccag ctcgtcgacg
     7921 acaagaagct tgtaccctgc ggcgagagcg ccatcgagat cgaattcggt cgtgggccgc
     7981 ccattgccgc tcatcgcctt gcggggcagc tcgggaaggc cggccgtcag ctcttcggtg
     8041 tccttgcgcc cgtgggtttc gacgattccg atccccaccg cgacgccctc ggcctggagc
     8101 tggcgcgcgg cctgaagcat ggcgaaggtc tttcccacgc ccgcgcaggc gccgaagaat
     8161 atcttcagct gcccgcgttt ggagcgatgg gcttcgccct tcacggcctc cagaagatcg
     8221 tcgggatcgg gtcgttgatc cttcatcatt cccctccaac ctcgataccg tcttccactt
     8281 ccggtcggag agtttccatc agaagaatgg ggtcagcaga atatcgatca gctttatacc
     8341 gataaaggga gctatgatcc cgcccaatcc gtagatcaac aggttcctgc tcaacaggcg
     8401 cgccgccggc tctgcccgat atttgacgcc tttcaaggcg agcggaataa gcaccacgat
     8461 gattagcgca ttgaagatca ccgccgccag gattgcggag gccggactgt ggaggcccat
     8521 gatgttgagg gccgccagag gcgggtaggt cgtgacgaac gcagccggga tgatggcgaa
     8581 atatttcgcc aggtcgttgg cgatgctgaa cgtcgtcaag gcgccgcggg tcatgatcat
     8641 ctgcttgccc acctcgacca cgcgcaggag cttcgtcggg ttgctgtcga ggtcgaccat
     8701 gttgccggcc tccttggcgg cctgggtgcc gctgttcatc gccacggcaa cgtcggcctg
     8761 cgccagcgcg ggcgcgtcat tggtgccgtc gccggtcatg gcgaccagcc gcccgtccgc
     8821 ctgatattcg cggatgaggc gcagtttgtc ctccggcgtc gcttccgcga ggtagtcgtc
     8881 gacaccggcc tcggccgcga ttgcggccgc cgtcagcttg ttatcgccgg tgatcatcac
     8941 cgtcttgatg cccatcgcgc gtagctcggc gaagcgctcc ttgatgccgc ccttgacgat
     9001 atccttcagc tcgacgatgc cgagcacctt gccgccatcg gcgacgacga gcggggtgct
     9061 gccacgcctg gacacctcgt cgacggtctt gccgatctcg ccggggaaga cgccgcccag
     9121 cgattcgaca tgcgcgcgga tggcgctcgc cgcgcccttg cggatttggc ggccgtcgat
     9181 gttgatgccg ctcatgcggg tgtgggcgct gaaagcgatc gcctcggcgc catgcagttc
     9241 gcggccgcgc aggttgaact tctgcttcgc cagcacgacg atggaccggc cttccggcgt
     9301 ctcgtcggca agcgaggcaa gctgcgctgc gtcggccagc gtctgttcgc tgacctgtgc
     9361 ggctggaatg aactgggctg cctcgcggtt gccaagcgtg atggttccgg tcttgtcgag
     9421 caggagcacg tcaacgtcgc ccgccgcttc gatggcgcgg ccggaggtgg cgagcacatt
     9481 cgccttcatc attcggctca tcccggcgat gccgaccgcc gagagcaatg cgccgatggt
     9541 cgtcgggatc aggcagacca acaaggcgat aagcaccgtg atggtgacca ccgaaccgcc
     9601 accggcgaca acggtgctga agctggagaa cggcaaaagc gttacgcaca ccagaaggaa
     9661 gatcagcgtg agggcgacca gcaggatggt gagggcgatc tcgttgggcg tcttgccacg
     9721 cttggcccct tcgaccatgg cgatcatgcg gtcgaggaag gcttcgcccg gcctggccgt
     9781 aacgcgcatg atgatccagt cggacaaaac gcgcgtgccg ccggtgaccg agcagaagtc
//
LOCUS       DASYCS010000169         3004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2647296,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000169 DASYCS010000000
VERSION     DASYCS010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2647296"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1143)
                     /gene="glp"
                     /locus_tag="VGM46_05425"
     CDS             complement(<1..1143)
                     /gene="glp"
                     /locus_tag="VGM46_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019858176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEY4192064.1"
                     /translation="MGLIPVAEALAHLLDGVEPLPGESVPLSQAMDRVLSEPIMALRT
                     QPPFDASAMDGYAVRAEDIATLPARLAVVGTAPAGRGFTGRVGQGETVRLFTGAPVPA
                     GADTVVIQENVHDLGNGIIEVLQPAAKGINIRRAGLDFAKGDLLLEKGRQLDPAALAL
                     AASANHPSLKVVRQPLVAIIATGDELLAPGSEPGPGQIISSNAYGVAAVAHSVGARSL
                     DLGIAPDRKEAIAALMRQAVDAGADVLVTLGGASVGDHDLVHDVLTGEGMVLDFWKVA
                     MRPGKPLMSGRLDNIRCIGLPGNPVASLVCSQLFLKPLLARLGGRPHRADLRAAKLGA
                     AMAANDHRQDYIRATLREEEDGPVATPFGIQDSSMLRRLADAHCLIV"
     gene            complement(1145..1639)
                     /gene="moaC"
                     /locus_tag="VGM46_05430"
     CDS             complement(1145..1639)
                     /gene="moaC"
                     /locus_tag="VGM46_05430"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006334526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEY4192065.1"
                     /translation="MPRAGAPALTHLGAQGEAHMVDVGDKADTSRTAIAEGFVTMRPE
                     TLSMILDGNAKKGDVLGTARIAGIMAAKKAHELIPLCHPLLLTKVSVDIEPDHALPGL
                     KVTALARVTGKTGVEMEALTAASVACLTIYDMAKAVDRAMVISNVRLVEKTGGKSGAY
                     KAET"
     gene            complement(1632..2447)
                     /gene="trpC"
                     /locus_tag="VGM46_05435"
     CDS             complement(1632..2447)
                     /gene="trpC"
                     /locus_tag="VGM46_05435"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEY4192066.1"
                     /translation="MADILRKIEAYKRDEIAAAKARAPLAELKARAADADAPRGFLAA
                     LEAKRKAGLFGLIAEIKKASPSKGLIRADFDPPSLAKAYQSGGAACLSVLTDEPSFQG
                     APEFLTQARGAVALPALRKDFLFDPYQVHQARAWGADAILIIMACVDDAMAHEMEETA
                     AALGMDALIEVHDEAELERALKLRSRLIGINNRNLRTFETNLDVSERLATMVPADRLL
                     VSESGIFTHDDCKRLEKSGIGTFLIGESLMRQKDVAQATATLLTGTASAAAHA"
     gene            complement(2452..>3004)
                     /locus_tag="VGM46_05440"
     CDS             complement(2452..>3004)
                     /locus_tag="VGM46_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEY4192067.1"
                     /translation="PSHHPAMKHVGPTRVELGTRTIFNILGPLSNPAGVTRQLVGVFS
                     PEWVVPVAETLKALGSEHVWVVHGDGYDEITTTGETQVAELIGGKISNFTLTPESVGL
                     KRWNKQDLHGGDARYNAEALRAMLGGAPGAFRDTVLMNAGAGLIVAGKVTTLTDGMVA
                     AAQAIDSGRALAVLDRLAKISNE"
BASE COUNT          492 a          972 c          994 g          546 t
ORIGIN      
        1 cacgatgagg caatgggcgt cggccagcct gcgcagcatc gaggaatcct gaattccgaa
       61 cggcgtcgca acaggcccgt cctcctcttc gcgcagcgtc gcgcggatat agtcctgccg
      121 gtgatcgttg gcggccatgg ccgcgcccag ttttgccgcg cgcaaatcgg cccgatgcgg
      181 gcggccgccg agccgggcaa gcaggggctt caggaaaagt tgtgagcaga ccaggctggc
      241 gacgggattg ccgggaaggc cgatgcagcg gatgttatcc agccggcccg acattagcgg
      301 ctttcccggc cgcatcgcca ctttccagaa atccagcacc atgccttcgc cggtcaacac
      361 gtcatgcacg aggtcgtggt cgccgaccga tgcgccgccc aatgtcacca gcacatccgc
      421 gccggcatcg acagcctgtc tcatgagcgc cgcgatggct tccttgcggt cgggcgcgat
      481 gccgaggtcg agcgagcgag caccgacgga atgcgccacg gcggccacgc cataggcgtt
      541 ggaggagatg atctggccgg ggccaggttc gctgcccggc gccaacaatt cgtcgccggt
      601 ggcgatgatg gccacgagcg gctgcctgac gaccttcaag cttgggtggt tggccgaagc
      661 cgccagcgcc agcgccgccg ggtcgagttg ccgacccttt tcgagcagca ggtcgccctt
      721 ggcgaaatcc agcccggcgc ggcggatgtt gatgcccttg gcggccggtt gcaacacctc
      781 gatgatgccg ttgccgaggt cgtggacatt ttcctggatg acgacggtgt ctgcgcccgc
      841 aggtacgggt gcgccggtga acaggcgcac ggtttcgcct tggccgacac ggccggtaaa
      901 gccgcgtcct gccggcgccg tcccgacgac ggctagcctg gccggaaggg tggcgatgtc
      961 ttcggcgcgc acggcgtagc cgtccattgc ggaagcatcg aaaggcggct gcgtgcgcag
     1021 cgccataatc ggctcgctca gcacgcgatc catggcttgc gaaagcggca cgctctcgcc
     1081 gggcagcggc tcgacgccgt caagcagatg tgcgagggct tcggcgaccg ggatcaggcc
     1141 catctcaggt ctcggccttg taggcgccgg atttgccgcc ggtcttttcg acgagcctga
     1201 cattcgagat caccatggcg cggtccaccg ccttggccat gtcgtagatg gtcaggcagg
     1261 caaccgaggc ggcggtcagc gcctccatct caaccccggt cttgccggta acgcgggcaa
     1321 gtgcggtcac tttcaagccc ggtaaggcgt gatccggctc gatatcgacg gaaaccttgg
     1381 tcaaaagcaa cggatggcac agcggaataa gttcgtgcgc cttcttggcg gccatgatgc
     1441 cggcgatgcg cgcggtgccc agcacatcgc ccttcttggc gttgccgtcc aggatcatcg
     1501 acagcgtttc cggccgcatg gtgacgaacc cttcggcaat cgccgtgcgg ctcgtgtcgg
     1561 ccttgtcgcc aacgtcgacc atgtgggcct cgccttgcgc gccaaggtgg gtgagggcgg
     1621 gagcgccggc tctaggcatg tgcagcggcg gaggccgtcc cggtcaagag cgtcgccgtc
     1681 gcctgcgcga cgtctttctg ccgcatcagg ctttcaccga tcaggaaggt gccgatgccg
     1741 ctcttttcca gccgcttgca gtcatcatga gtgaaaatgc cgctttcgct gaccagcagg
     1801 cggtcggccg gaaccatcgt ggccagtcgt tcggaaacat cgagattggt ctcaaatgtc
     1861 cgcaaatttc ggttgttgat gccgataagc cgggagcgca gtttcagcgc gcgctcaagc
     1921 tcggcttcgt catgcacctc gatcagcgcg tccatgccga gcgcggcggc ggtttcttcc
     1981 atctcgtgcg ccatggcatc gtccacgcag gccatgatga tgagtatggc gtccgcgccc
     2041 caggcgcgcg cctggtggac ctgataggga tcgaacagga aatctttccg caaggcgggc
     2101 agggcgactg ccccgcgcgc ttgcgtgagg aactcaggcg cgccctgaaa cgacggctcg
     2161 tcggtcagca cggaaagaca tgccgccccc ccgctctgat aggctttggc cagcgaaggc
     2221 ggatcgaaat cggcgcggat caggcccttg gacgggctgg ctttcttgat ctcggcaatc
     2281 aggccgaaca gtcccgcctt gcgcttggct tccagcgcgg ccagaaagcc gcgcggcgca
     2341 tcggcatccg ccgcccgcgc cttgagttcg gccagcggcg cgcgcgcctt ggcggcggcg
     2401 atctcgtcgc gcttgtaggc ttcgatcttg cgcaatatgt cggccatggc gttactcgtt
     2461 cgaaatcttt gccagtctgt ccagcacggc cagcgcgcgg ccgctgtcga tggcctgcgc
     2521 cgccgcaacc atgccatcgg tcagcgtggt gaccttgccg gcaacgatca ggcccgctcc
     2581 cgcgttcatc agcacggtgt cgcggaaagc acccggcgcg ccgccaagca tggcgcgcaa
     2641 cgcctcggca ttatatctgg cgtcgccgcc gtgcaaatcc tgcttgttcc agcgtttcaa
     2701 gccgacggat tcgggtgtaa gggtgaagtt gctgatcttt ccgccgatca gttccgccac
     2761 ctgcgtttcg ccggtcgtgg tgatctcgtc atagccgtcg ccatggacga cccagacatg
     2821 ctcggagccg agcgccttca gcgtttcggc gacaggcacc acccattcgg gcgagaacac
     2881 gccgaccagt tgccgggtga cgcctgccgg attggaaagc ggtccgagta tattgaagat
     2941 ggtgcgggtg ccgagttcca cgcgggtcgg cccgacatgc ttcatggccg gatgatggga
     3001 tggc
//
LOCUS       DASYCS010000170        11791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4821765,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000170 DASYCS010000000
VERSION     DASYCS010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 11791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11791
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4821765"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1184)
                     /locus_tag="VGM46_05445"
     CDS             complement(<1..1184)
                     /locus_tag="VGM46_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006332221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEY4192068.1"
                     /translation="MDDNDKKYPRKSGPKPAGPRARSAGAAQDRKPGGKPAFGAKKPF
                     VKRDGPNKPYAPRGDRPAAADGERPKRDFAPRGGGEAERPRSEFKREYKPREPGEAPR
                     GERTFNKGPRPEGKPFVKRDGPRKPYAPRGDRPAAAEGERGERRFDRPKRDDAPGFGR
                     DSGRSGQSGGSSSGFKPRPRPADAAEEAGERIAKRLARAGLASRRDAEELIEAGRVKV
                     NGKVLASPAFNVMPGDIIHLDGMEIPPIERTRLFLFHKPAGVVTTNRDPEGRKTVFDV
                     LPADLPRLMTIGRLDINTEGLLLLTNDGGLSRVLELPATGWLRRYRVRVHGKVEESAL
                     TGLREGIAVDGVFYGAIEATLDREQGSNAWLTLALREGKNREVRNILGSLGLDVTRLI
                     RIS"
     gene            1278..1730
                     /locus_tag="VGM46_05450"
     CDS             1278..1730
                     /locus_tag="VGM46_05450"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006332219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEY4192069.1"
                     /translation="MKRPDFMALALGEARAAALRGEVPVGAVVVLDGAVIAAAGNRTR
                     ELSDPTAHAEMLAIRRACATLASERLTGCDLYVTLEPCAMCAGAISFARLRRLYFGAG
                     DEKGGAVVSGPRFFASPTCHHAPDVYPGMAETESAIILRDFFAERREP"
     gene            complement(1774..2469)
                     /locus_tag="VGM46_05455"
     CDS             complement(1774..2469)
                     /locus_tag="VGM46_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008875113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192070.1"
                     /translation="MTERHHARLMLLAPLVVSALALSGCMSSPTYGTDKTSSEQLADD
                     LSSAFSFAPQKKEHIDYKPRPALVKPAAGQKEALPVPQDSITQTASNEWPESPEQRRA
                     RLRADATAHQDDPNYQAQIVDDDPNAVPTADNYKVAQLGHDRAPAWTPEDSGEGTAAR
                     RAEISKRLQEQKQGDPTTRKYLSEPPVDYRVAASTAPQGDIGEDEYKKERRLKKEAAA
                     KGGKKGMFDWLPW"
     gene            complement(2672..5590)
                     /gene="ileS"
                     /locus_tag="VGM46_05460"
     CDS             complement(2672..5590)
                     /gene="ileS"
                     /locus_tag="VGM46_05460"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEY4192071.1"
                     /translation="MTDTAETIDYSKTLYLPQTEFPMRAGLPEKEPLLVKRWQDMDLY
                     SKLREDAKGRTKYVLHDGPPYANGNIHIGHALNKILKDVITRSFQMRGYDANYVPGWD
                     CHGLPIEWKIEEQYRAKGKNKDEVPVNEFRQECREFAAHWIGVQGAEFQRLGVIGDFK
                     NPYATMAFHAEARIAGELLKFAMSDQLYRGSKPVMWSVVERTALAEAEVEYQDYESDT
                     IYVKFPVAGDASLADAHVVIWTTTPWTIPGNRAVNFSPRINYGLYEVTAAENTFGPQP
                     GEKLVFADALVEAASEKAKVTMRLLRSVSAQELEGLTLSHPLKGLGGGYEFPVPMLAG
                     DHVTDDAGTGFVHTAPGHGREDFDAWMDAAPELRKRGVDTAIPFTVDDAGYLTKDAPG
                     FGPDREGGAARVIDDNGKKGNANQAVIDELIKRNALFARSRLKHSYPHSWRSKKPVIF
                     RNTPQWFIYMDKDLADGTTLRSRALKAIDDTRFVPPAGQNRIRAMIEERPDWVLSRQR
                     AWGVPIAIFADADGNVLKDEAVNARITEAFEKEGADAWFAEGARERFLGGRADESWKK
                     VTDILDVWFDSGCTHAFTLEDRPDLKWPADVYLEGSDQHRGWFHSSLLESCGTRGRAP
                     YDTVVTHGFTIDEEGRKMSKSLGNTVVPQDVMKQSGADILRLWVMTTDYWEDQRLGKN
                     VLQTNIDAYRKLRNTIRWMLGTLAHDDGEEVPLRDMPELERLMLHRLAEMDELVRSGY
                     DAFEFKRITRALLDFMVVELSAFYFDIRKDALYCEGPSSVKRKASVQVVRHLFDAIVK
                     WLAPMLPFTTEEAWADRHPGAVSVHLEQFPAIPAEWRDEELAEKWRKVRQVRRVVTGA
                     LEIARAQKVIGSSLEAVPVVYLNDQALEAAIADVDMAEMAITSDLEIAHGAPPSGAFA
                     LEDVKGVAVTVDKAEDRGLRKCARSWRYIADLGSDPEFPDVSARDAAVLHELKALGRL
                     "
     gene            complement(5819..6628)
                     /locus_tag="VGM46_05465"
     CDS             complement(5819..6628)
                     /locus_tag="VGM46_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019857478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HEY4192072.1"
                     /translation="MRLVSLFSAALAASVAVAPVAAKAADGAFGWLSGDWYLSLGASG
                     IVAPNFEGGSKYLFSAAPLVSLGKAGNETRFSSRNDNISLSLFDNGGVRAGLVGKFIF
                     GRDNDADELKGLDPVRWGGEIGGFAEFYPTDWLRVRGEVRQGIRSHHGVVADVTADAF
                     YDVTPTVRLSGGPRLSFASASYFDAYYGVDATESAASGLSQYDPGGGLKSAGVGGAVT
                     WKVTDPITASLFGEYSRLTGPAADSSLVRERGDKNQFMFGLSTTYRFDFTM"
     gene            6803..7357
                     /locus_tag="VGM46_05470"
     CDS             6803..7357
                     /locus_tag="VGM46_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HEY4192073.1"
                     /translation="MTLSGDTLKDAIGHTRAKWGWFVALGVLLLIFGGIAFGNLFVAT
                     VASVYVIGWLMLMAGAVEIIHAFCVKSWGRFFYWLFSGLLYAVAGFFAFDNPLLASAV
                     LTFLLAIALIASGLLRVWVAYQHRAEKGSGWIITAGIVTALAGLVIAMGWPVNSLWVL
                     GLFLAIDLVFQGWTFIAFGLALKK"
     gene            complement(7365..8348)
                     /locus_tag="VGM46_05475"
     CDS             complement(7365..8348)
                     /locus_tag="VGM46_05475"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEY4192074.1"
                     /translation="MATPCERFPGSTPLPDRLRGGVVAIGNFDGVHRGHQAVLDRALA
                     QAHRLGVPALALTFEPHPRKVFRPDVPLFILTPPSMKAKLLSKLGFSALVEQPFTREF
                     SGLSAQAFVTEVLERNLGISHVVTGFNFHFGKDRQGGPAYLIEAGKRHGFGVTLVDAF
                     RDEGAEVVSSSRVRALLSEGDPAQAAGLLGYRYTVEGEIVGGEKLGRTLGFPTANIRL
                     SPESTLKEGIYAVRFRRADGTLYDGVASYGRRPTVDDDGAPLLETFVFDFTGDLYGEV
                     CDVSFFAYLRGEIKFDGLEALVVQMKRDEAEARALLAGVKPLSELDAAIAF"
     gene            complement(8357..9217)
                     /locus_tag="VGM46_05480"
     CDS             complement(8357..9217)
                     /locus_tag="VGM46_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01459 family HAD-type hydrolase"
                     /protein_id="HEY4192075.1"
                     /translation="MANPPVTISSLVELERRYAAIFCDVWGVVHNGVVAFSDAAQALS
                     AIRASGLAVVLVTNSPRPHEGVEAQLRALGVPDTAWDRVVTSGDVTRDLIASVPRRIF
                     HLGPERDHPIFEGLDVELVEDFEANAVVCTGLFDDEVDTPEDYKELLTRLRARDLPFI
                     CANPDIIVERGEQMIWCAGSLARDYAQLGGRTLIAGKPHASIYAAALKAAASVLGREV
                     ARSEVLAIGDGMMTDIKGASDNGIDALYISGGIHAGEHGDASNPDAAMLAAFLDKHGY
                     RPVATMPKLR"
     gene            9482..11380
                     /locus_tag="VGM46_05485"
     CDS             9482..11380
                     /locus_tag="VGM46_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEY4192076.1"
                     /translation="MIRKFLNDRSGNYALMTVITMVPLMGALALGIDYTDLTRQRQDT
                     INALDAAGLATARRLTEGATDQQAIDYANQFFRANLKIADADDATLAVLLPSANTGGG
                     TLKLTAQMTFQPYFLPVASWLIGKSSGSTNINFNAVSEVRLKNTLEVALVLDNSGSML
                     DPGKDSSKVRFDLLKDAATQLVDQLAGQAKQMKQIQKPVQFSLVPFAASVNVGPDNAT
                     ESWMDTTGVSPIHHENLDWSTLSKTLNAQKYAEKDKSTGIWYARGTSWDATQKDKPLT
                     RFSLYNLMQRVSKISNKGVYTYDTVASWGGCVEARPSPYDVDDTPASTGTPATMFVPM
                     FAPDETDSSSGGRPANNNWHLDANPKSNQSDLVLQKYVPKYFNSGTSVTRAYGMDEGP
                     NMSCSTTAITPLTDVSTPDGLTVITKAIDNMVADGATNVPEGMAWGWRTLSSAAPFTG
                     GRSEVGKGNDKVLIVLTDGANTYYTPDSVQGQSYSGSGYTSGANDLAGNKSIYSALGY
                     AAKAADSIYSYGRIFLGTSSSVSKTTYTNANYTKAMNEHFTTLCNNAKANNLIVMTIA
                     LDLDKTKSADLTQMNALKACASDSRVRRDETDPTKPAKLFWNSTGKTLSDDFKQIGNE
                     LSNLRIVS"
     gene            complement(11441..>11791)
                     /locus_tag="VGM46_05490"
     CDS             complement(11441..>11791)
                     /locus_tag="VGM46_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010915462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HEY4192077.1"
                     /translation="TAFLHEVLTTDRGWTLIIAGNAVGFVFAVIALATTVVAFPLLLD
                     RDVGAVAAIETSARAVMANPVQMALWGIMVAFLLGLGSLPLFAGLAVVMPVLGHATWH
                     LYRKLVEPERPRPM"
BASE COUNT         2260 a         3707 c         3611 g         2213 t
ORIGIN      
        1 gaaatgcgga taagccgggt gacgtcgagg ccgagagagc cgagaatatt cctcacctcg
       61 cggttcttgc cttcacgcag cgccagcgtc agccaggcat tcgatccctg ctcgcggtcc
      121 agcgtcgcct cgatcgcgcc ataaaacacg ccatcgacgg caatgccctc gcgcaggccg
      181 gtcagcgcgc tttcctcgac cttgccgtgg acgcggacgc ggtagcgccg cagccagccg
      241 gtcgcgggca gttccagcac gcgcgaaagg ccgccgtcat tggtaagaag cagcaggccc
      301 tccgtattga tgtcgagccg cccgatggtc atcaggcgcg gcaggtcggc gggcagcacg
      361 tcgaaaaccg tcttgcggcc ttccggatcg cggttggtgg tgacgacgcc agccggcttg
      421 tggaacaaaa acaggcgcgt gcgttctatc ggtggaattt ccattccatc gagatggatg
      481 atgtcacccg gcatcacgtt gaaagccggg gacgccagca ccttgccgtt gaccttgacg
      541 cggcctgcct cgatcagctc ctcggcatcg cggcgggagg ccaggccggc gcgcgccagc
      601 cgcttggcga tgcgttcgcc cgcctcttca gcggcatccg caggacgtgg gcgcggcttg
      661 aaaccgctcg atgagccacc cgactggccg gacctgcctg aatcgcggcc aaaacccggc
      721 gcatcatcgc gcttgggtcg gtcaaagcgc ctttcgccgc gttcgccctc ggccgctgcg
      781 gggcggtcgc cgcgcggcgc ataaggcttg cgcgggccat cgcgcttgac gaacggcttt
      841 ccttcgggac gcggaccctt gttgaacgtg cgctcgccgc gcggcgcctc gccgggttcg
      901 cgaggcttat attcacgctt gaattccgat cgcggacgtt cggcttcccc gcccccgcgt
      961 ggggcgaaat cccgtttagg acgctcgcca tctgcggcag ccgggcggtc gccacgcggc
     1021 gcgtagggtt tgttcgggcc atcgcgcttg acgaacggct tcttcgcacc gaaggccggc
     1081 ttgccgccgg gcttcctgtc ttgcgccgct ccagcggagc gggcacgagg gccagccggt
     1141 ttcgggccgc tcttgcgcgg atatttcttg tcgttgtcgt ccattgtggc ctttgctcgt
     1201 ttgcgcgggt agtaacagga tagcggccaa gtggcgagga tataagtgcc agaaggacga
     1261 ccggaatgga aacccgcgtg aagcggcctg acttcatggc cctggcattg ggcgaagccc
     1321 gcgccgcggc tttgcgcggc gaggtgccgg tgggggctgt cgtggtgctt gacggcgcgg
     1381 taatcgctgc cgccggcaac cgcacgcgtg aactgtcaga cccgacggca catgccgaaa
     1441 tgctggcgat acggcgggcc tgcgcgacac ttgccagtga aaggctgacg ggctgcgatc
     1501 tctatgtgac gctggagccg tgcgccatgt gcgccggcgc catctcgttt gcgcggctgc
     1561 gccgcctcta tttcggcgcg ggtgacgaga aaggcggggc cgtcgtcagc gggccacgct
     1621 tctttgcgtc gcccacctgc catcacgcac ccgatgtcta tccgggcatg gccgaaacag
     1681 aatcagcaat catcctgcgt gatttctttg ccgagcggcg cgagccgtga gcgcaacccg
     1741 ccgggaaaaa gctccggact acaaagctgc gaactaccag ggcagccagt cgaacatgcc
     1801 cttcttgccg cccttggcgg cggcttcctt cttgaggcgg cgttcctttt tgtattcatc
     1861 ctcgccaata tcgccttgag gcgcggtgct ggcggcgacc cggtagtcca ccggcggctc
     1921 cgacaggtat tttcgcgtgg tcggatcgcc ctgcttctgt tcctgcaagc gcttggatat
     1981 ctcagcgcgg cgcgcggcgg tgccctcgcc tgaatcttca ggcgtccagg ccggcgcgcg
     2041 gtcatggccc aactgggcga ccttgtagtt gtcggccgtt ggaaccgcat tgggatcgtc
     2101 gtcaacgatc tgggcctggt aattcggatc gtcctggtgc gcggtggcat ccgcgcgcag
     2161 gcgggcgcgg cgctgttcgg gcgattccgg ccattcgttg ctggcggtct gggtaatgct
     2221 gtcctgcgga accggcagcg cttccttctg gccggcggcc ggcttgacca gcgcggggcg
     2281 tggcttgtag tcgatatgct ctttcttttg cggagcgaac gaaaaggcgc tgctcagatc
     2341 atcggcaagc tgctcgctgg acgtcttatc ggtgccgtag gtcggggaac tcatgcagcc
     2401 cgacagcgcc agtgcggaca ccacaagcgg cgccagcagc atcaggcgcg catgatgtct
     2461 ctcggtcatg ccaaaaagtc ccacttcaca aacctctgat tgcttgccgc cttctgaccg
     2521 ctgccagaag gtccggacta cccgtcccgc tcactgtacg gagaatccgg cgacaatttg
     2581 tcttacggag ttttgcgctt ttacctcaac cgctcgttaa gggcaacgcg ccggcgaacc
     2641 aattgcccgg cgcgttgcat ttgcgcttcc gttacaggcg gccgagcgcc ttcagttcgt
     2701 gcagcacggc cgcgtcgcgc gccgagacat cgggaaactc cgggtcgctg ccaagatcgg
     2761 cgatgtagcg ccacgaacga gcgcatttgc gcaggccgcg atcttccgcc ttgtcgaccg
     2821 tcacggcgac gcccttcacg tcttccagcg cgaaagcccc ggaaggcggc gcgccatgcg
     2881 cgatttcgag atcgctggtg atcgccatct cggccatgtc gacatcggcg atggcggcct
     2941 ccaaagcctg atcgttgaga tagacgaccg gaaccgcctc aagcgaggag ccgatgacct
     3001 tttgcgcgcg cgcaatttcc agcgcgccgg tgacgacgcg gcgcacctgg cgcaccttgc
     3061 gccacttttc cgccagttcc tcgtcgcgcc actccgctgg aatggcgggg aactgctcca
     3121 gatgcacgga aaccgcgccg gggtgccggt cggcccaggc ttcttccgtg gtgaagggca
     3181 gcatcggcgc cagccatttg acgatcgcgt cgaacagatg gcgcaccacc tgcaccgaag
     3241 ccttgcgctt cacgctcgac ggcccttcgc aataaagcgc atccttgcgg atatcgaaat
     3301 aaaaggccga aagctcgacc accatgaagt cgagcaaagc gcgggtgatg cgcttgaact
     3361 caaacgcatc gtagccggaa cgcaccagct catccatttc tgcaagccgg tgcagcatca
     3421 gccgctccaa ctccggcatg tcgcgcagcg gcacttcctc gccatcgtca tgggcaagcg
     3481 tgcccagcat ccagcggatg gtgttgcgca acttgcgata agcgtcgatg ttggtctgaa
     3541 gcacgttctt gccgaggcgc tggtcctccc agtagtcggt cgtcatcacc cacaggcgta
     3601 gtatatcggc gccggactgc ttcatcacat cttgcggaac gaccgtattg ccgagcgatt
     3661 tcgacatttt gcggccctcc tcatctatgg tgaaaccatg ggtgacgacg gtgtcgtaag
     3721 gcgcgcggcc acgcgtgccg cagctttcga gcagcgagga atgaaaccag ccgcgatgct
     3781 ggtccgagcc ttccagatag acgtcggccg gccatttcag gtctgggcgg tcttccagcg
     3841 tgaaggcatg cgtgcagccg gaatcgaacc agacatcgag aatgtcggtg actttcttcc
     3901 aggattcatc ggcgcggccg ccgaggaagc gctcgcgggc gccttcggca aaccaggcgt
     3961 ccgcgccttc cttctcaaac gcctcggtga tgcgggcatt gaccgcttca tccttcagca
     4021 cattgccatc ggcatcggcg aagatagcga tcggcacgcc ccaggcgcgc tgacgcgaaa
     4081 gcacccagtc cgggcgctcc tcgatcatgg cgcggatacg gttctggccg gcggggggca
     4141 cgaagcgggt atcgtcgatg gccttcagcg cgcggctgcg cagggtcgtg ccatcggcga
     4201 ggtccttgtc catgtagatg aaccactgcg gcgtgttgcg gaagatgacc ggtttcttcg
     4261 agcgccacga atgcggatag ctgtgcttca ggcggctgcg cgcaaacagc gcgttgcgtt
     4321 tgatcagttc gtcgatcacc gcctggttgg cattgccctt cttgccgttg tcgtcgatga
     4381 cgcgcgccgc gccgccctcg cggtccggcc cgaagcccgg cgcgtccttg gtcaggtagc
     4441 cggcatcgtc gacggtgaag ggaatggcgg tatcgacgcc gcgtttgcgc aattcgggcg
     4501 cagcatccat ccaggcatcg aaatcctcgc ggccatggcc gggcgcggta tgcacgaaac
     4561 cggtgccggc atcgtcggtg acgtgatcgc cggcaagcat gggaacggga aattcataac
     4621 cgccgccgag gcccttgagc gggtgtgaaa gcgtaagacc ttcaagctct tgcgccgaaa
     4681 cgctgcgcag gagcctcatg gtcaccttcg ccttttcgga ggccgcctcg accagggcgt
     4741 ccgcgaaaac gagcttttcg ccaggctgcg gtccaaaagt gttttcagcg gccgtaacct
     4801 cgtaaagccc gtaattaatg cgtggcgaga aattgaccgc gcggttgccc ggaatggtcc
     4861 atggcgtcgt cgtccagatg acgacatgag cgtccgcaag gctggcatcg cccgccaccg
     4921 ggaacttcac gtagatcgtg tcgctctcgt aatcctggta ttcgacctcg gcctcggcca
     4981 gcgcggtgcg ctcgaccacg ctccacatca ccggcttgga gccgcgatag agctggtccg
     5041 acatggcgaa tttcaacagt tcgccggcga tgcgcgcctc ggcgtggaaa gccatggtcg
     5101 cgtacgggtt cttgaagtcg ccgatgacgc ccagccgctg gaattccgcg ccctgtacgc
     5161 cgatccagtg ggcggcgaat tcgcggcatt cctggcggaa ctcgttgacc ggaacctcgt
     5221 ccttgttctt gcccttggcg cggtactgct cctcgatctt ccattcgatc ggcaggccgt
     5281 ggcaatccca gcccggcaca taattggcat cgtaaccgcg catctggaac gagcgcgtga
     5341 tgacatcctt caggatcttg ttgagcgcat ggccgatatg gatgttgccg ttggcatagg
     5401 gcgggccgtc atgcagcaca tatttggtgc ggcccttggc gtcttcgcgc agctttgaat
     5461 aaaggtccat gtcctgccag cgcttcacca acagcggctc cttttcaggc aggccggcgc
     5521 gcatgggaaa ctccgtctgc ggcaaataaa gcgtcttgga ataatcgatc gtttcagcgg
     5581 tatcggtcat tggtctgccc gtggtctgcc atgtgcattg cccgtcggca aaagcctcgg
     5641 gcgttgaact atcatgatat ggaaaaagcg cgaggggtct gcgcaaaaac ccggcggctc
     5701 cggcggcctt caggccaccc agaacaccgg gcaggtaatt cgtatcgcga aagcgcgcgt
     5761 catgatggcg cttctagcgg atcgagcagc gggaataaag cttttcctgc cgccgccact
     5821 acatcgtgaa atcgaaacga taggtggtgg aaaggccgaa catgaactgg ttcttgtcgc
     5881 cgcgttccct gaccaggctg gaatcggcgg caggtccggt cagcctggaa tattcgccga
     5941 acaggctggc ggtgatcgga tcggtgacct tccaggtgac agcgccgccg acgccggcgg
     6001 atttcaggcc gccgccggga tcgtactggc taagaccgga agcagcggat tcggtcgcat
     6061 cgacgccgta ataggcatcg aaatagctgg ccgatgcgaa ggaaaggcgt gggccgcccg
     6121 acagccggac tgtcggcgtg acgtcataaa acgcgtccgc cgttacatcg gccacgacgc
     6181 cgtgatgcga gcggatgcct tgccgcactt cgccgcgcac ccgcagccaa tcggttggat
     6241 aaaactcggc gaagccgccg atttcaccac cccagcgcac cgggtccagc cccttcaatt
     6301 catcggcgtc gttgtcgcgg ccgaaaatga actttccaac caggccggcg cgcacgccgc
     6361 cattgtcgaa cagcgaaagc gaaatattgt cgttacggga agaaaaccgc gtctcgtttc
     6421 cggccttgcc cagcgaaacc aggggtgcgg cgctgaacaa atatttgctg ccgccctcga
     6481 agttgggcgc aacgatgccg gaagcgccaa ggctcagata ccagtcgccg gacagccagc
     6541 cgaacgcgcc gtcggccgct ttggccgcaa caggggcgac ggcaacagaa gcggccagcg
     6601 ccgccgaaaa cagggaaacc aaacgcatcg ccactccgta cacgcactgc aactcacggt
     6661 cgatcagcat tgaggcaaag gtggtaaagt ttgatttaaa cctgcaataa gtcgacggtc
     6721 gccgcccgct ggaccaaagc ggcggttgcg attatcaatt cacatgcctt ggcgatcggc
     6781 gtcgggcgag aaggaggcta cgatgacact cagcggcgat accctgaaag acgccatcgg
     6841 gcatacgcgc gccaaatggg gctggttcgt cgcgctgggc gtgctcctgc tcatcttcgg
     6901 cggaattgcc ttcggcaacc tgttcgtcgc cacggttgct tccgtctatg tcatcggctg
     6961 gctgatgctg atggccggcg cggtcgagat catccacgcg ttctgcgtga agtcgtgggg
     7021 ccggttcttc tattggctgt tcagcggcct gctctatgcc gttgccggct tctttgcctt
     7081 cgataatccg ctgcttgcct cggcggtgct gacattcctg ctggcaatcg cgctcatcgc
     7141 ctccggtttg ctgcgagtat gggtcgcgta tcagcatcgg gcggaaaagg gatcgggctg
     7201 gatcatcacc gccggcatcg tcacggcgct ggccggcctt gtcatcgcca tgggctggcc
     7261 ggtcaacagc ctgtgggtgc tcgggctgtt cctggccatc gatcttgtct tccagggctg
     7321 gacttttatc gccttcggcc tggcattgaa gaaatagagg tcaatcaaaa agcgatggcg
     7381 gcgtcgagtt ccgacagggg cttgacgccg gccagcagcg cccgcgcctc cgcctcgtcg
     7441 cgcttcatct ggacgacgag tgcttccaga ccgtcgaatt ttatctcgcc gcgcagatag
     7501 gcgaagaacg atacgtcaca gacctcgcca tagagatcgc cggtgaagtc gaacacgaac
     7561 gtttccagca acggcgcgcc gtcgtcatcg acggtcgggc ggcggccata gctggcaacc
     7621 ccatcgtaaa gcgtgccgtc ggcgcggcga aagcgcactg cataaatgcc ttccttcagc
     7681 gtggattcgg gcgaaagcct tatgttggcg gttgggaagc cgagcgtgcg gccaagcttt
     7741 tcgccaccga caatctcgcc ttccacggtg tagcggtagc cgagcaggcc ggcggcctgc
     7801 gccggatcgc cttccgatag cagcgcacgg acccggcttg acgataccac ttcggcgccc
     7861 tcgtcacgaa aagcatcgac cagcgtgacg ccgaaaccat gccgtttacc ggcctcgatc
     7921 agataggccg gcccgccttg cctatccttg ccgaagtgga agttgaagcc ggtcacgaca
     7981 tggctgatgc cgaggttgcg ttccagcact tccgtcacga atgcctgcgc cgacagccct
     8041 gagaattcgc gcgtgaacgg ctgctcgacc aaggcggaaa agccgagctt ggacagcagc
     8101 ttcgccttca ttgacggtgg cgtcaggatg aacagcggca catcgggccg aaaaaccttt
     8161 cgcgggtgcg gttcgaaagt cagcgccaat gcaggaacgc cgagccgatg cgcttgcgcc
     8221 aatgcgcggt caagaaccgc ctgatgaccg cgatgcacgc cgtcgaaatt gccgatcgcc
     8281 acaacgccgc cgcgcagccg gtccggtagc ggcgtcgaac ctgggaaacg ctcgcagggc
     8341 gtcgccatgg cagcctctac cgcagtttcg gcatggtcgc gacaggccgg tagccgtgct
     8401 tgtcgagaaa agccgccagc attgccgcat cggggttcga tgcgtcgcca tgctcgcctg
     8461 cgtgaatgcc gccggaaata tagagcgcgt cgataccatt gtcggaagcg cccttgatgt
     8521 cggtcatcat gccgtcgccg atagccagga cttcgctacg cgccacttcg cggccaagca
     8581 ccgatgccgc cgccttcagc gcggccgcat aaatcgaggc atgcggcttg ccggcgatca
     8641 acgtgcgacc gcccagttgg gcatagtcac gcgcgagcga accggcgcac cagatcattt
     8701 gctcgccgcg ctccacgata atgtccgggt tggcgcagat gaacggcaaa tccctggcgc
     8761 gcaggcgcgt caacagttcc ttgtaatctt ccggcgtatc gacttcgtca tcgaacaatc
     8821 cggtgcaaac cactgcattg gcttcgaaat cctcgaccag ttcaacatcc aggccttcga
     8881 agatgggatg gtcgcgttcg gggccgagat ggaatatccg tcgcggaacg gatgcgatca
     8941 aatcccgcgt cacgtcgccg gaagtgacca cgcgatccca tgccgtatcc ggcacgccaa
     9001 gcgcgcgaag ctgcgcctcg acgccttcat gcgggcgtgg tgaattggtg accaaaacga
     9061 cggccagtcc gctcgcccgg atcgccgaca acgcctgggc ggcgtcggaa aaggcgacca
     9121 cgccattgtg aacgacaccc cagacatcgc agaatatcgc ggcgtagcgc cgttccagct
     9181 caacgagcga ggatatcgtg actggtggat ttgccatacc gccccaaatc tggacatggc
     9241 cgccgacggg cggcccgacg gcccggatta accgaacgca accgccctgt caccagcttt
     9301 cacgggcgat ctttccgcca ggccgccctg cgccggcggc aaccgctccg tgccggccgg
     9361 gcatggttaa cgcgccttga aacggccttg gcattttgaa gtttcattag gaacctgaaa
     9421 tttaacgatt tgtggcatcg tcgggcctta tgaaggggtc ctgcccaggg gttacaacgc
     9481 aatgatccgc aaattcttga atgacagaag cggcaactat gccttgatga cggttatcac
     9541 gatggtgccg ttgatgggcg cgctggcgct gggcatcgac tacaccgacc tgacgcgcca
     9601 gcggcaggac acgatcaacg cgctggatgc cgctggcctc gcgactgccc gtcgcctcac
     9661 ggaaggcgcc accgaccagc aggccatcga ctacgccaac cagttcttcc gggcaaacct
     9721 caagattgcg gatgcggacg acgcaacgct tgctgttctt ctgcccagcg ccaataccgg
     9781 cggcggtacg ctaaagctta ccgcccagat gacgttccag ccttattttc ttcccgtggc
     9841 ttcgtggctg atcggcaaaa gctccggctc caccaacatc aacttcaacg cggtgtcgga
     9901 agtacgcctc aagaacacgc tggaagtagc gctcgtgctc gataattcgg gttcgatgct
     9961 cgatccgggc aaggattcgt ccaaggtccg gttcgacctt ctcaaggatg ccgccacgca
    10021 gctcgtcgat cagctcgctg gtcaggccaa acagatgaag caaatccaga aaccggttca
    10081 gttcagcctc gttccatttg cggcctcggt caacgtaggc cccgacaacg cgaccgagtc
    10141 ctggatggat acgactggtg tctctccgat tcaccacgaa aacctcgact ggagcacgct
    10201 gagcaagacc ttaaatgccc agaaatatgc cgagaaagac aaaagcacgg gtatttggta
    10261 tgcgcgcggc acttcttggg atgcaacgca aaaggacaag ccacttaccc gcttttcctt
    10321 atacaatttg atgcagcgcg tttcaaagat cagcaacaaa ggtgtctaca cctacgacac
    10381 ggtagcctcg tggggcggct gcgttgaggc gaggccctca ccttatgatg tcgacgacac
    10441 tccggcttca acgggaacgc ctgcaacgat gttcgtgccc atgttcgctc cggatgaaac
    10501 cgatagcagc agcggcggca ggccggccaa taataactgg cacctggacg ccaatccgaa
    10561 atcaaaccaa agcgacctgg ttctccagaa gtatgtgccc aaatacttca attccggcac
    10621 gtcggtgacg cgagcgtatg ggatggatga aggtcccaat atgagttgca gcacgactgc
    10681 gattacaccg cttaccgatg tctcgacgcc agatggcctc actgtcataa cgaaggctat
    10741 cgacaatatg gtcgccgacg gcgcaacgaa cgtgccggag gggatggcat ggggctggag
    10801 gacgctttcc agcgcggcgc ccttcacggg cggtcgttcg gaagtcggca agggcaacga
    10861 caaggtgctg atcgtgctga ccgacggcgc aaacacctat tacacgccgg attcggtgca
    10921 gggtcagagc tactctggtt ccggttatac ctccggcgca aacgatcttg ccggtaacaa
    10981 gtccatctac tcagcgctcg gctatgcagc gaaagctgct gacagcatct atagttatgg
    11041 ccgcatattc ctgggaacca gcagcagcgt cagcaaaacg acctatacga acgccaacta
    11101 caccaaggcg atgaacgagc attttacaac gttgtgcaac aacgcgaagg ccaacaacct
    11161 gatcgtcatg acgattgccc tggacctcga caaaacgaag tcggcggacc tgacgcaaat
    11221 gaacgcgctg aaggcgtgcg cctcggattc ccgcgttcgc agagatgaaa ccgatccgac
    11281 caagccggcg aagcttttct ggaattcgac gggcaagacc ctgtccgacg acttcaagca
    11341 aatcggcaat gaattgtcca acttgcgtat cgtcagctaa gacacaaggt gcccggaacg
    11401 cagccgttcc gggcattggc tttcgccttg cctaccggcc tcacatcggt cgcggccgtt
    11461 ccggctcgac aagcttgcgg tacaggtgcc aggtggcgtg gccgagcacc ggcatgacga
    11521 ccgccagccc cgcgaaaagc ggcagcgagc cgaggcccag cagaaacgcc accattattc
    11581 cccacaaggc catctgcacc ggattggcca tcaccgcgcg ggctgaggtt tcgattgccg
    11641 ctaccgcgcc gacatcacgg tcgagcagca gcggaaaggc gaccacggtg gtggccagcg
    11701 cgatcaccgc aaagacaaag ccgacggcat tgccggcgat aatcagcgtc caaccccggt
    11761 cagtcgtcag cacttcgtgc aggaatgcag t
//
LOCUS       DASYCS010000171         4210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_5114923,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000171 DASYCS010000000
VERSION     DASYCS010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4210
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5114923"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..782
                     /locus_tag="VGM46_05495"
     CDS             102..782
                     /locus_tag="VGM46_05495"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY4192078.1"
                     /translation="MAFISKTLEADSASNSYRSGRIRLFNDIVATVLARKDRCRVLDV
                     GGTHAYWTAFGQAIDWSRVEVTLLNLEHIDMPRSAGIDQVVGDATDMREFDDDSFDIV
                     HSNSVIEHVGLWSSMEAMAGEIRRLAPAYFVQTPSFWFPVEAHSRTPFFHFLPEPLRV
                     ALIMRRKLGYWEKAADIGEATRTAHSAILLHKSQMRHLFPDAGIVVEKAYGFSKSYIA
                     IRKNPPQH"
     gene            complement(805..1575)
                     /locus_tag="VGM46_05500"
     CDS             complement(805..1575)
                     /locus_tag="VGM46_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007536094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY4192079.1"
                     /translation="MPDVTRLSVRDIRKSFGTHEVLRGISLEAKDGDVISVLGSSGSG
                     KSTFLRCINLLEIADAGEIWVDGEQIRMAQKAGRNRPASQKQVDHIRSELGMVFQSFN
                     LWSHMTILQNVIEGPVHVLKRPRAECVAEAEALLEKVGIGDKANAYPAHLSGGQQQRA
                     AIARALAMKPKVMLFDEPTSALDPELVGEVLRVMRALAEDGTTMLVVTHEMSFARNVS
                     SRVVFMKEGLIESAGSPDEMFGKGASPAFRQFIGHFGS"
     gene            complement(1594..2298)
                     /locus_tag="VGM46_05505"
     CDS             complement(1594..2298)
                     /locus_tag="VGM46_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007795665.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY4192080.1"
                     /translation="MDFAFLSSTMLTLIQALPMTLALFALSVTAGCLLALMIVWMRVG
                     GNRYLAAFAKAYIFVFRGSPLLIQMFLLFYGLAQFGFIRNGPLWPLLREPFVCAVLSL
                     ALCTAGYSAEIFRGGIRSVPPREIEAARSIGMSGFLLVRRIIAPIAFRFALPAYSTEV
                     VLMMKSTSLASLVTVWEVTGVAQRLISHTYRTMEVFLCAALIYLILNFIVLQGMALLE
                     YALSRHRRAAPPARKA"
     gene            complement(2308..3030)
                     /locus_tag="VGM46_05510"
     CDS             complement(2308..3030)
                     /locus_tag="VGM46_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012555285.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY4192081.1"
                     /translation="MSMLELLGFGSTGWGALLLVATGVTLAVTAVALVIGAVIGTIVA
                     SAKLSGNFFLVTLGSIYTTVFRGVPELLIIYLIYFGGSSAITAIGQSLGYEGFLGLPS
                     FAAGALAVGIISGSYQAEVFRGAYLAISKGELEAASAIGMNHGLRLRRIIMPQVLRYA
                     LPGIGNVWQLSLKDSALISVTGLAELMRTSQVAAGSTRQYFLFFIVGGALYLVLTSLS
                     DQLFNRAERRANRSMPSAMGQV"
     gene            complement(3201..4040)
                     /locus_tag="VGM46_05515"
     CDS             complement(3201..4040)
                     /locus_tag="VGM46_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018482975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY4192082.1"
                     /translation="MKIQTILLTGALALSSFAAPAMAKDWKKVTITLEGAYAPWNMTN
                     SDGTLGGFEPELAKVLCERAKVECTLVASDWDGMIPALNAGKFDIIMDALSITDERKQ
                     VIDFTVPYAATPAAFATAKDSPLANAAGTGTSVKMDVGQTGVKEIDALKEAFKGKTIG
                     IQAATVYAKFVYDNFGDTATVREYKTGADRDLDLQNGRIDLGFDDGVYFESAFEAAKG
                     ALAFTGPEIVGPIWGAGEGLGVRKADTDLRDMFSAAIKSALDDGTVKTLSMKWFKTDV
                     SPK"
BASE COUNT          832 a         1347 c         1301 g          730 t
ORIGIN      
        1 caatacccca actggatagc tttttgcgtt ggccaatcgc cgtcatactc cactcccaag
       61 cgcgccgggc tggaccttga agcggctcgg gacgggcgaa tatggcattc atctcaaaaa
      121 cactggaagc ggactcggcc agcaacagct accggtccgg ccgcattcgc ctgttcaacg
      181 acatcgttgc gaccgtgctg gcgcgcaagg accggtgccg cgtgctcgat gtcggcggca
      241 cccatgccta ctggacagcg ttcggccagg ccatcgactg gtcgcgcgtc gaggtgacgc
      301 tcctgaacct cgaacatatc gacatgcccc gttcggcggg catcgatcag gtggtcggcg
      361 acgccaccga catgcgcgaa ttcgacgacg acagcttcga catcgttcat tccaactcgg
      421 ttatcgagca tgtcggcctg tggagttcga tggaagcgat ggccggcgag atccgccgcc
      481 tggcgccggc ctatttcgtg cagacgcctt cgttctggtt cccggtcgag gcgcattcgc
      541 gaacgccgtt tttccacttc ctgccggaac cgctgcgcgt cgcgctgatc atgcggcgca
      601 agcttggcta ttgggagaaa gccgctgaca taggcgaggc gacgcgcacc gcccacagcg
      661 ccatcctgct acacaaaagc cagatgcgcc atctgttccc cgatgccggg atcgtcgtcg
      721 aaaaggccta tggcttcagc aaatcctata tcgcgatccg caagaacccg ccgcagcact
      781 gacgagatcg cggcaggcgg acgctcagct gccgaaatgc ccgatgaact gccggaaagc
      841 cggcgaagcg cctttgccga acatttcgtc gggcgagccg gcgctttcga tcagcccttc
      901 cttcatgaag acgacgcggc tggagacatt gcgcgcgaag ctcatctcgt gggtgaccac
      961 cagcatggtc gtgccgtctt cggccagtgc gcgcatcacg cgcagcactt ctccgaccag
     1021 ttcgggatcg agcgccgagg tcggttcgtc gaaaagcatc accttgggct tcatggcgag
     1081 cgcgcgcgcg attgccgcgc gctgctgctg gccccccgaa agatgtgccg gataggcgtt
     1141 tgccttgtcg ccgatgccga ccttttccag cagcgcctct gcctcggcca cgcattcggc
     1201 gcgcggccgc ttcagcacat gcaccggccc ctcgatgaca ttttgcagga tggtcatatg
     1261 cgaccacaga ttgaacgact ggaacaccat gccgagttcg gagcgaatgt ggtcgacctg
     1321 cttctgactg gcagggcggt tacggccggc tttctgcgcc atccggattt gctcgccatc
     1381 cacccagatc tcgccggcat ccgcgatctc aagcaggttg atgcagcgca ggaaggtcga
     1441 cttgccggac ccgctcgacc cgagcaccga aatgacatcg ccatccttcg cttccagcga
     1501 aatgccgcgc agcacctcgt gggtgccgaa gctcttgcgg atgtcgcgaa ccgaaagacg
     1561 ggttacatcg ggcatgaacg ctccattcgt ttctcacgcc ttccttgccg gcggcgcggc
     1621 gcgccgatgg cgggagagtg catattcgag cagggccatg ccctgcaaga cgatgaagtt
     1681 gaggatgaga tagatcagcg ccgcgcacaa aaacacttcc atcgtgcggt aggtatgtga
     1741 aatcagccgc tgggcgacgc cggtgacctc ccataccgtg accaggctcg ccagcgatgt
     1801 cgatttcatc atcagcacca cctcggtcga ataggcgggc agcgcgaagc gaaaggcgat
     1861 cggcgcgatg atccggcgca ccagcaggaa gccggacatg ccgatggaac gcgccgcctc
     1921 gatctcgcgc ggcggaaccg agcggatgcc gccgcgaaag atttccgccg aatagcccgc
     1981 agtgcacagc gccagcgaca gcacggcaca gacgaacggc tcgcgcagca gcggccacaa
     2041 cggcccgttg cggatgaagc cgaattgcgc caggccgtag aacagcagga acatctggat
     2101 cagcagcggc gagccgcgaa acacgaagat gtaggccttg gcgaaagccg ccagatagcg
     2161 gttgccgccg acgcgcatcc acacgatcat cagcgccagc aggcagccgg cggtgacgga
     2221 aagcgcaaac agcgccagcg tcatcggcag cgcctggatg agcgtgagca tggtcgagga
     2281 gaggaaggcg aaatccatgg ccgctcctca gacctggccc attgccgacg gcatcgaccg
     2341 gttggcgcgc cgttccgccc ggttgaacaa ctggtcggaa aggctggtga ggacgagata
     2401 gagcgcgccg ccaacgatga agaacaggaa atattgccgc gtcgaaccgg ccgcgacctg
     2461 gctggtccgc atcagctcgg caagcccggt caccgagatc agcgccgaat ccttcaggct
     2521 caactgccag acattgccga tgcccggcag cgcgtagcgc agtacttgcg gcatgatgat
     2581 gcggcgcaaa cgcaagccat ggttcatgcc gatggccgac gccgcctcca gctcgccctt
     2641 cgaaatggcg agataggcgc cgcgaaagac ttccgcctgg taggaaccgg aaatgatgcc
     2701 gacggcaagc gcgccggcgg cgaaggacgg caggccgagg aacccctcat agcccagcga
     2761 ctgcccgatg gcggtgatgg cagaggagcc accgaaatag atcagataga tgatcagcag
     2821 ttcaggcacg ccgcgaaaaa cggtggtgta gatgctgccc agcgtcacca aaaagaaatt
     2881 gcccgacagc tttgccgaag cgacgatggt gccgataacc gcgccgataa ccaacgccac
     2941 cgccgttacg gccagcgtca cgccggtcgc caccagaagc agcgcacccc atccggtaga
     3001 gccaaagcca agcagttcca acatcgacat tgcagagctt tcccgccgcg cagcgcgtca
     3061 acgccatgga agaatagcat gggtcccccg ttgcgaaagc gggaaacccg gattccaaag
     3121 ccttatctca ggcccctgtt cgaagacggg cgccgccggc gcagggtcgg cggggtccgt
     3181 ccaggattca tgtccaggat ttatttcggg gacacgtcgg tcttgaacca cttcatcgac
     3241 agcgtcttga cggtgccgtc atccagcgcc gacttgatcg ccgcgctgaa catgtcgcgc
     3301 aggtcggtgt cggccttgcg cacgccaagg ccttcgcccg cgccccagat cggcccgacg
     3361 atttccggcc cggtgaaggc cagcgcgccc ttggccgcct cgaaggcgct ttcgaaataa
     3421 acgccatcgt cgaagccgag gtcgatgcgg ccgttctgta gatcgaggtc gcggtcggcg
     3481 ccggtcttgt attcgcgcac cgtggcggtg tcgccgaaat tgtcatagac gaacttggcg
     3541 tagacggtgg ccgcctggat accgatggtc ttgcccttga aggcctcctt cagcgcatcg
     3601 atttccttca cgccggtctg gccgacatcc attttcacgc tggtgccggt gccggcggca
     3661 ttggccagcg ggctgtcctt ggcggtggcg aaggcagccg gcgtcgccgc atagggaacg
     3721 gtgaaatcga tgacctgctt gcgctcgtcg gtgatcgaca gcgcatccat gatgatgtcg
     3781 aacttgccgg cattcagcgc cgggatcatg ccgtcccagt cggaagccac cagcgtgcat
     3841 tcgaccttgg cgcgttcgca cagcaccttg gcgagttccg gctcgaagcc gccgagcgtg
     3901 ccgtcggaat tggtcatgtt ccatggcgca tacgcgcctt cgagcgtgat cgtcaccttc
     3961 ttccagtcct tggccatcgc tggcgcggca aagctggaca gcgcgagcgc gccggtcaga
     4021 agaattgtct ggattttcat tggtctctcc tccgaagctg tagctggccc ttggccacat
     4081 agggtccgat cttgaagtcg gcttgcccgc gccggaccgg ccccgtgccg acctcgtttc
     4141 gggttattat acaaggtgat atgggggcag cctgcgctgt gcaagcgaaa agtcggtcgc
     4201 tccgcgagca
//
LOCUS       DASYCS010000172        15443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1197918,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000172 DASYCS010000000
VERSION     DASYCS010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 15443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15443
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1197918"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(362..1591)
                     /locus_tag="VGM46_05520"
     CDS             complement(362..1591)
                     /locus_tag="VGM46_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEY4192083.1"
                     /translation="MSVVPLQQNSAQPDAIDRLVAERRPGYGLVRSFYHDAAIFERDM
                     ERVFRRHWHCLAHESVIPNTGDFELFNLGDEAVILTRAADGAVHAMLNVCRHRGAEVC
                     TKQKGNAKMFVCPYHAWTYSNDGSLRAARLMPKDFDRAEHGLKKLNVRVAAGLVFISF
                     AEKPLDFDPIEQSLRTSAGQYGWGEAKVAHRQMYSFEANWKLAVENYVECYHCGPAHP
                     EYSQTHALEKPKEMIEQLNAAMEERTCALGISISSGNRWQNSAGGQEAIDTFRYALYD
                     GVASGSKDGRLVAPPMGRFTQSDGGVTSIHLGGTSFLVCYPDHGMIYRFVPKGPNSCD
                     MELIWLVRGDAVEGQDYDLEKLTWLWTVTTEEDKKIIEHTARGVRSHYFVPGPIAPME
                     YNELRYIDWYLEEISRA"
     gene            complement(1807..2160)
                     /locus_tag="VGM46_05525"
     CDS             complement(1807..2160)
                     /locus_tag="VGM46_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006336820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY4192084.1"
                     /translation="MTRATIFKFGAVENAETGDLPGWTVVEGSPTMKTTVQHTTEDGK
                     VLSGTWQATPGTYHATYTDYEFVHMIAGRIIITPDGGKSVEVGPGDAFVVEADFKGSW
                     KIIEPVTKHFVVTVG"
     gene            2397..3278
                     /locus_tag="VGM46_05530"
     CDS             2397..3278
                     /locus_tag="VGM46_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018425890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEY4192085.1"
                     /translation="MEVITAQQARQANEQYREEGYAIVRGFVDGAKIERLQAEARSVY
                     DEGLKHHATYRNGNLSFQVLPESHFDKRYVLQAYWFSWINSYFEEMRRGADYLALLEG
                     LLGRDIKQVAQQLHWKPPGVNVSGYRFHQDIRFRKGADMDKVAASTVTTGLAVDRATP
                     ENGCLQVVPGSHKLGYLGLSDDTNDGMLMKGLTAEEELHAAGIDPASIVNLELEPGDL
                     ALWGLLTVHGSAPNNSQHDRAFLLSSYVDAGASERGEWAFRDGVSTPLGDTPKLCKDD
                     RILKDTGPVYDTTAWWN"
     gene            complement(3311..4219)
                     /locus_tag="VGM46_05535"
     CDS             complement(3311..4219)
                     /locus_tag="VGM46_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018425889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY4192086.1"
                     /translation="MNLLTALKVFERLATLGSLSAAGRDLGMSSTAISRHLKDLEDDL
                     GARLVNRTTRKLSLTEVGSNYLDRARVLLDGVDELQDTARGLHNRTRGLIRVSCSNVL
                     GHTRITGLVPKFLEENPLTTVEFNLTSRYIVGIVEEGYDVAIRFGAQTDSSLIARRLG
                     EVRNYVCASPEYFLRHGRPAHPADLAGHACVLSNYAKRMGVWPFHGPDGPFSAMVEGR
                     VSTNSVEAAYQMTLAGCGIGNLPSLLVEPAIRDKRLEVVLDEFRPSSSPIYALYPHRN
                     YVPAKVRAFVEFLMRELAPEIANGPS"
     gene            complement(4660..5736)
                     /locus_tag="VGM46_05540"
     CDS             complement(4660..5736)
                     /locus_tag="VGM46_05540"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HEY4192087.1"
                     /translation="MSSHNIAVIGGDGIGTEVVEAGVQVLEALAAAEAGLSFDFKRFD
                     WGSDYYRRNGRMMPQDGLDQIKGFDAIYFGAVGDPNLPDDITLWGLRLAICQGFDQYA
                     NVRPTRLLPGLTGPLRPDLGKQIDWVIVRENSEGEYAGVGGRAHRGLPIEVGMDVAVF
                     TRAGIERIARFACDLASSRPRKLLTVVTKSNAQRHGMVLWDEVVREVLADYPGLTVDW
                     MLVDAMTQRMVNKPGSIDTVLATNLHADILSDLAAALGGSLGIGSTSNVDPSRHRPSM
                     FEPIHGSAFDIMGKGVANPLGSFWTACLMLEHLGEPAAAKRLMAAIEVVTARGEHLSP
                     DLGGKANTAEVTQAVVAALRGDNA"
     gene            complement(5748..6815)
                     /locus_tag="VGM46_05545"
     CDS             complement(5748..6815)
                     /locus_tag="VGM46_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007753499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEY4192088.1"
                     /translation="MAHLTVGILNGDDIGHEIVPASVRIAKAAAARAGLEIDWRPMPI
                     GRSALDSLGTTMPEGTLEKLSALDGFILGPIGHRAYPKAAGAINPHPILRKHFGLFAN
                     VRPTRSFPGLGALFDDVDLVIVRENNEGFQPDRNVVAGSGEFRPTEDTTISVRVITRA
                     GSYRVAKAALELARTRRKKLTLVHKDTVFKLGCGMFVEECYRAAEAFPDVEVDEVIVD
                     TFAMRLLRDPQTFDVVVTTNMFGDILSDEAAGLVGGLGMAPGLCIGEGNIAMAQAAHG
                     SAPDIAGKGIVNPYAMIESTRMLLEWLGHNRGIEQAVAAASMMQAATVSALSQPETRT
                     GDIRGTTDTEGMTRAILAAIG"
     gene            complement(6861..8270)
                     /locus_tag="VGM46_05550"
     CDS             complement(6861..8270)
                     /locus_tag="VGM46_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY4192089.1"
                     /translation="MHDVVDAGLTKYVAEFVVDAKPQDLPEDVVALGKKSILDGIGLA
                     LSGSVAQSGALVRRHLDDIGLIPGSSTVIGAGRKVAPRFAAFANGVGIHADDYDDTQL
                     AVATDRVYGLLTHPTAPALPAALAMGEALNASGAKVMLAYHLGVEVECKIAEAIAPRH
                     YQTGFHATATCGTFAAASAAASLLGFDVETTARALSIAGSQSAGLRENFGTMTKPFHA
                     GRSSESGVAAAQFAAIGWTATDKILEAPRGFFNAAGGGYDSASIRGRLGDPWTFANPG
                     ISIKPHPSGSLTHPGMTEMLRLIKEHGIKAKDVKHVRIGTNSNMPNALIHHRPHDELQ
                     AKFSMEFCMAILLLDGKAGLGEFTDATTQREDVKAMIEKVDFVIDEKAEKAGYHKMTT
                     IIDVTLDDGRVIHGEADFGKGSPANPMSYEEVAYKFRDNAAHARLARAAADEVVSLVR
                     DLESQPSLDRLMALVAKAG"
     gene            complement(8584..9291)
                     /locus_tag="VGM46_05555"
     CDS             complement(8584..9291)
                     /locus_tag="VGM46_05555"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEY4192090.1"
                     /translation="MAKQSEQLSGVSPIDFLRTRSLANVVQHEIERMIVEGEFTPNER
                     INENALAQQLKVSRGPIREACSALAAVGLIQVIPNRGFFVRELSEEEARDVAEARAYV
                     FACMTMSVAERITDKEVTRLRALVDRMDNLAKTGEVSQYYPVNLEFHAELARMCGNSR
                     LAQIYLGLAKELHIQRYRALASGDVLDISNHEHRAIVDAVAARDPQRAFAAGRAHIVN
                     GSTRTRRATAVLRHVVE"
     gene            complement(9538..11268)
                     /locus_tag="VGM46_05560"
     CDS             complement(9538..11268)
                     /locus_tag="VGM46_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEY4192091.1"
                     /translation="MTIAAVSPDPSLGRRRFKLDLSTPLFVLLAVVLCVLVLLPLFWL
                     GFYAFTDKSGSPTLANFVKLLTDATIRRAFLISLAMAGCVGFFSCLVATPLAWLVART
                     DMPGQRLVRSLVTASFVTPPFLGAIAWVMLAAPNSGILNNIYRWAFGLDQSQSLFNIY
                     TFTGLVFAITCYSFPFVFTLVANAFDKVPSDLEDASAILGGKPGTTLRRVTLPMVLPA
                     MLAGSLVAIVQAFTMFGSPAILALPAGFHVITTKIWSYFQFPPRLDLAAASAVPLLIV
                     TMLLLWGQKWLLGRRGYTVLGGKSGTPRKVQLGPWKWLAGAYVLFILCITIVFPYLAL
                     LKVALTHNISEPLSWSSLTLHNFHFVLFEFSSTRLAIWNTLILGLSTATIATGMALVI
                     AYLVARRPVRGAPLLGMLATAPIAIPGIVLGVGLFLAYANPTFGLYGTLWILLIAFMT
                     IELPAGYQQLSSAFLGVHPELEEASRILGATRLQSLWQITAPLLRTSLVAAWSFVFIG
                     TIRELSATVLLTTAPTKLVSVIIYDLNESGDYGAIAVLGILLLVVSFAVVFLANRVPV
                     LGGMNERSGG"
     gene            complement(11506..12552)
                     /locus_tag="VGM46_05565"
     CDS             complement(11506..12552)
                     /locus_tag="VGM46_05565"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY4192092.1"
                     /translation="MSNSKVAAGALFAALAWSQVAWAAPPEPYTVTDDLVAAATKEAS
                     VVYYTSTDVQVAEKLGAAFEKHYPGIKVQVERAGSERVFQRIGQEYSSGIHNADVIET
                     SDASHFLTFKDQNWLTPLVPEDVAKMWPEKDKDPDGMYAAFRATLTVIAYRTDKVKEE
                     DAPKTWNDLLDPKYKDKLVKSHPGYSGNVMTATYALSQLLGWDFFEKLGKQGVMQVQS
                     STEPPKVLAQGERNIQADGNEYNVFILQDKGVPIKAVYPPEGTSIAYGNAAVLAYAPH
                     PNAAKLFYSFMFTQEAQQLNDDFGGLRSVRPDVKEKSNRTPLSQIKLLDSDPAKVRDA
                     MEEIKQKYEEYFGT"
     gene            complement(12564..13631)
                     /locus_tag="VGM46_05570"
     CDS             complement(12564..13631)
                     /locus_tag="VGM46_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4192093.1"
                     /translation="MAFVELDKLTKRYGKVTVVNGVDLAIEKGTLVCLLGPSGCGKTT
                     TLRLVAGFVQPSDGQIRVGGRVVSSASASEPPEARNMSMIFQSYALWPHMTVFENVAY
                     GLRLRKMAKSEIQAKTKRILQATKLDGLAERYPGELSGGQQQRVSLARALVIEPETLL
                     LDEPLSNLDANLREEMRFEIRRLHDEFRYTTVYVTHDQTEAMTAADIIVVMNQGNVEQ
                     AGSPEDIYERPQSEFVAQFIGGTNIFKGNRIGDNAVDVGNGLILKCGSGDFAANGPTA
                     VSVRHHDLHLVGEKPSGDLENWATGKVVRQVYLGSHRDYLVSLSGGETVRAIAPVGVA
                     IPENQQVWLHCPPDRCRALAH"
     gene            complement(13783..14319)
                     /locus_tag="VGM46_05575"
     CDS             complement(13783..14319)
                     /locus_tag="VGM46_05575"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEY4192094.1"
                     /translation="MKDDNLLLTMLLVFAPLSLMAIGGGASVLGELQRSVVDNQHWLT
                     NQGFLDMFAISRVAPGPGTLIVTLIGWHLAGLLGAIVSTIAIFAPSSMLVVVVAYFWH
                     RHPNAAWQKALIYGLAPLAAGLVLSSVFALLQEARGGWIAWFVAILVAVASRKVRLGP
                     LTLMGAGAIIFCGLLALQ"
     gene            complement(14316..14915)
                     /locus_tag="VGM46_05580"
     CDS             complement(14316..14915)
                     /locus_tag="VGM46_05580"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEY4192095.1"
                     /translation="MPQADLPALKPASPKPEEPALPKPGLLQLFLVFTKLGITSIGGG
                     VNGMMHSEFVIHRRWFTDEQFLSGLALSQSLPGVNVANLALWLGYQCRGIIGAVVALA
                     AVLLPPAFVIILIGDVVLRMTNVPEIGSLLAGLAVAATGLSLSVGYLATRYACTDIAS
                     VLLFVGTFIGATAFHFSALVVVAVLAPIGIGIGFLRNRS"
     gene            15175..>15443
                     /locus_tag="VGM46_05585"
     CDS             15175..>15443
                     /locus_tag="VGM46_05585"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY4192096.1"
                     /translation="MRSELEFLQKHSLTSVLQRELETLILSGEFSPGERLNENALAAR
                     FSVSRGPVREVCRALAEKGLLELIPNRGVFMRRIDEAEAAELYDLR"
BASE COUNT         3007 a         4934 c         4693 g         2809 t
ORIGIN      
        1 gatcttctgg ccgatctcgt tggcaagcgc gctgtatacg ctggggcgcg tttcccctgg
       61 cgcgcgtctg ctgcgcgcct tgcccgtact ggctttcccg accgccatgc tgcctctttg
      121 cctggaaatg gaggctacag gcctatgcaa ccgaaagcga cccccaagcg gcgccccgaa
      181 actgccaaca tcgtaagccc ccggcggccg ttcaacaccc tcgcaggcgt tgaccgaatt
      241 caactcccga accggaagag caacggcccg caaccgcttg tctggaatcg cgaatccctg
      301 cgggtcggca ccggccccgt tttccgggaa gggtggcgaa cggcaaggcc gccagcactt
      361 ctcaggcgcg ggaaatttct tccagatacc agtcgatata gcgcagctca ttatattcca
      421 tcggggcgat cggccccggc acgaaataat gagagcgcac gccgcgcgcc gtatgctcga
      481 tgatcttctt gtcttcctcg gtggttaccg tccacagcca ggtgagcttt tcgaggtcgt
      541 agtcctgccc ctcgaccgca tcgccccgga ccagccagat caattccatg tcgcagctgt
      601 tcggcccctt gggcacgaag cgatagatca tgccatggtc ggggtagcag acgaggaagc
      661 tcgtgccgcc gagatggatc gaggtcacgc cgccatcgga ctgcgtgaag cggcccatcg
      721 gcggcgcaac cagcctgcca tccttgctgc cgctggcaac gccgtcatag agcgcatagc
      781 ggaacgtatc gatagcctcc tgcccgcctg ccgaattctg ccagcgattg ccgctggaaa
      841 tgctgatgcc gagcgcgcag gtgcgctcct ccatcgccgc gttcagctgc tcgatcatct
      901 ccttcggctt ttccagcgcg tgcgtctgcg aatattccgg gtgcgccggg ccgcaatgat
      961 agcactcgac atagttctcg accgccagct tccagttggc ctcgaaggaa tacatctggc
     1021 gatgcgccac cttggcctcg ccccagccat actggccggc gctggtgcgc agcgactgct
     1081 ctatcggatc gaaatcgagc ggcttttcgg caaaggagat gaacaccagc ccggctgcga
     1141 cgcgcacatt gagcttcttc agcccatgtt cggcacggtc gaaatccttc ggcatcagcc
     1201 gcgccgcgcg cagcgatccg tcattggaat aggtccaggc atgataggga cacacgaaca
     1261 tcttcgcgtt gcccttctgc ttggtgcaga cctccgcgcc gcgatggcgg cagacgttga
     1321 gcatggcatg aacggcgccg tccgccgcgc gcgtcaggat caccgcctcg tcgccgaggt
     1381 tgaacagctc gaaatcgcct gtattgggga tgacgctttc atgcgccagg caatgccagt
     1441 ggcgccggaa cacccgctcc atgtcgcgct cgaagatcgc cgcatcgtgg tagaacgacc
     1501 gcaccaggcc gtagcccggc cggcgctccg ccaccaggcg gtcgatggcg tcgggttggg
     1561 ctgaattttg ctggagagga accacgctca tcgcaacttc cttgtctggc cttgatgtgc
     1621 cggcattcgg gcttaaaaac acccgcttgg cggccctttc gtgactaagc ctgagaaaac
     1681 ccgtcttcca aagagccagt ttagccgaga aattgcggca ccggtacagc taaccatcga
     1741 ggccgcctgc gcgttgccat gaaagcgacg ccctccctgc cggaaggcga cggccgccat
     1801 ggctttctag cccacggtga cgacgaaatg cttggtcacc ggctcgatga tcttccagct
     1861 tcccttgaaa tcggcctcga ccacgaaagc gtcgcccgga ccgacctcga ccgacttgcc
     1921 gccatcgggc gtgatgatga tgcgcccggc aatcatgtgc acgaactcat agtcggtata
     1981 ggtcgcgtga taggtgccgg gcgtcgcctg ccaggtgccg gacagcacct tgccgtcttc
     2041 ggtcgtatgc tggacggtgg tcttcatggt cgggctaccc tcgacaaccg tccatcccgg
     2101 aaggtcgccg gtctcggcgt tttcaaccgc accgaatttg aagattgtag cgcgtgtcat
     2161 cgtcatgctc cgttgggctt taaccggcca gttcaggaag cgaggatact tgcagctggc
     2221 gcaaaagact atgcgttcgg ctgaaatgaa cgccctggcc caaaccgatt ttccccgcag
     2281 gatcaggcgg ctgcgcgaac tatcccgaaa accgcaaagg tcgttcccca ttttgccctc
     2341 ttaatcgatg cctgcgatgc ggcgtagcct gtggtcatcg gcagtttcag ggagcaatgg
     2401 aagtgattac cgcacagcaa gctaggcaag ccaacgaaca ataccgcgag gaaggctacg
     2461 ccatcgtgcg cggcttcgtc gatggcgcga agatcgagcg ccttcaggcc gaagcccgct
     2521 cggtctatga tgaaggcctg aaacaccatg ccacctatcg caatggcaat ctgagctttc
     2581 aggtgctacc ggaaagccat ttcgacaagc gctacgtgct tcaggcctat tggttctcat
     2641 ggatcaacag ctatttcgag gagatgcggc gcggagccga ctatctggcc ttgcttgaag
     2701 gattgctcgg ccgcgacatc aagcaggtcg cccagcaact tcactggaag ccgccgggcg
     2761 tgaatgtcag cggctaccgc ttccatcagg atatccgctt ccgcaagggt gccgacatgg
     2821 acaaggtcgc cgcctcgacc gtcaccaccg gactggccgt cgaccgcgcc accccggaaa
     2881 acggctgcct gcaagtcgtt cccggaagcc acaagcttgg ctatctcggc ctgtcggacg
     2941 acaccaatga cggcatgctg atgaagggcc tgaccgcgga ggaggaactt cacgccgccg
     3001 gcatcgatcc ggcctcgatc gtcaatctcg aactggaacc cggcgacctg gctttgtggg
     3061 gactgctgac cgtccatggc tcagcgccca ataattcgca gcacgaccgc gccttccttt
     3121 tgtccagcta tgtcgatgcg ggtgcctccg agcgcggcga atgggccttt agggatggcg
     3181 tatcgacgcc gcttggcgac acgccgaagc tttgcaagga cgacaggatt ctgaaagaca
     3241 ccggcccggt ctacgacaca accgcctggt ggaactgaac gcgcgcatca aacgaaacgg
     3301 gccttcggcg tcacgaaggc ccgttcgcta tttccggcgc cagttcccgc atcaggaatt
     3361 ccacgaaggc ccgcaccttc gccggcacgt agtttcgatg cggatagagc gcatagatcg
     3421 ggctggagct gggccggaac tcatccagca ccacctccag ccttttgtcg cgtattgccg
     3481 gctcgaccag caggcttggc agattgccga tgccgcagcc ggcaagcgtc atctgatagg
     3541 cggcttcgac gctgttggtc gaaacccgtc cctcgaccat ggccgaaaac ggtccgtccg
     3601 gcccatgaaa cggccagacg cccatacgtt tggcataatt gctgagaacg caggcatgtc
     3661 ccgccaggtc ggcaggatgc gccgggcggc catgccggag aaaatattca ggcgaagcac
     3721 agacatagtt gcgaacctcg cccagacggc gcgcgatgag ggacgaatcc gtttgtgccc
     3781 caaaccggat cgcgacatcg tagccctcct cgacgatccc gacgatatag cgggaggtca
     3841 aattgaactc gacggtggtc agcgggtttt cttccaggaa tttaggcacc aggccggtaa
     3901 tccgcgtatg gccaagcacg ttggaacagg aaacgcggat cagcccccgc gtcctgttgt
     3961 gcaggccgcg cgccgtatcc tgtagctcgt ctaccccgtc gagaagcacc ctcgcccggt
     4021 ccagatagtt gctgccgacc tcggtgaggc tgagctttcg cgtcgtccgg ttgaccagcc
     4081 gcgcgcccag atcgtcctcc agatccttga ggtgacggct gatcgccgtc gacgacatgc
     4141 cgaggtcccg gcccgctgcg gaaaggctgc caagcgtcgc cagacgctcg aacaccttca
     4201 acgccgtcag aagattcatt gcctgccccc gccatagccg cgttcggcca gccaaccaga
     4261 aatgcgccac gcatggaagc cgccatgttc ctgtgcgcgg agtatgccga tggctgaatt
     4321 cgaaatcagg aaaacttgaa tgggctggat cgggtcgccg ccggcatcgg cctacgcctt
     4381 ggccggctgc ggcaagcttt gagaaccatt gccgccgcta tggaagcgga cagccatcat
     4441 ggcacgaagc cgctcgctgc aagcgttgca atcgccagtg caggatgccg actttgattg
     4501 gatatcactg ggaaatttcc ggctatcctg gctaatgccg cttggttcgg agcacatatt
     4561 gtcttccctc gtttcaggaa aacaatatgg ccgccggtca gccgtcagaa cccgcaaagc
     4621 gcgggatcga tccttgcgaa aatcgggaca aacgctttgt caggcgttgt cgccgcgcag
     4681 cgccgccacc acggcttgcg tgacttcagc cgtgttggcc ttgccgccga ggtcggggct
     4741 gagatgttcg ccgcgcgcgg tcacgacttc tatcgccgcc atcagtcgct tggcagccgc
     4801 cggttcgccc agatgctcaa gcatcaggca agcggtccag aacgagccga gcggattggc
     4861 gacgcccttg cccataatgt cgaaggccga gccatggatc ggctcgaaca tcgagggacg
     4921 gtggcgcgaa ggatcgacat tggatgtcga gccgatgccc agcgatccgc ccaaggctgc
     4981 ggccaggtcc gaaagaatgt cggcatgcag attggtggcg agcaccgtgt cgatgctgcc
     5041 cggcttgttg accatgcgct gcgtcatcgc atcgaccagc atccagtcga cggtcagccc
     5101 gggataatcg gccaacactt cgcgcaccac ctcatcccac agcaccatgc catggcgctg
     5161 cgcattcgac ttggtgacga cggtgagaag cttgcgcgga cgcgacgacg caaggtcgca
     5221 ggcaaagcgc gcgatgcgct cgatgccggc gcgggtgaac acggccacat ccatgccaac
     5281 ctcgatcggc aggccgcgat gcgcccgccc gccgacgccg gcatattcgc cctcgctgtt
     5341 ttcgcgcacg atcacccagt cgatctgctt gccgaggtcc gggcgcagcg gcccggtcag
     5401 gccgggcagc agccgcgtag ggcgcacatt ggcatactgg tcgaagccct ggcagatggc
     5461 gaggcgcagg ccccacagcg tgatgtcgtc gggaaggttc gggtcgccga ccgcgccgaa
     5521 atagatagcg tcgaaaccct tgatctggtc gaggccatcc tgcggcatca tgcggccatt
     5581 gcggcggtaa tagtccgaac cccagtcgaa gcgcttgaaa tcgaacgaca ggccagcctc
     5641 cgccgccgca agcgcttcca gcacctgcac gccggcctcg acgacttccg ttccgatgcc
     5701 atcgccaccg atgaccgcga tattatgcga ggacattgct gttttcctta tccaatagcc
     5761 gcgagtatcg cccgcgtcat tccttcggtg tcggtcgtgc cccgaatgtc gccggtgcgc
     5821 gtctcgggct gcgacagggc cgaaaccgtt gccgcctgca tcatcgaagc cgccgccacc
     5881 gcctgctcga tgccccggtt atggccgagc cattccagca gcatccgcgt cgattcgatc
     5941 atcgcatacg ggttgacgat gcccttgccg gcaatgtcgg gcgccgagcc atgcgctgcc
     6001 tgcgccatgg cgatgttgcc ttcgccgatg cacaagccag gcgccatgcc cagcccgccg
     6061 acaaggcccg ccgcctcgtc gctcaaaatg tcgccgaaca tgttggtggt gacgaccacg
     6121 tcgaacgtct gcgggtcgcg caatagccgc atggcgaaag tgtcgacgat gacctcatcg
     6181 acctcgacat ccggaaacgc ctcggcagcg cggtaacatt cttccacgaa catgccgcag
     6241 ccaagcttga acaccgtatc cttgtgcacc agcgtcagct tcttgcgccg ggtgcgggca
     6301 agctcaagtg ctgccttggc gacgcggtag ctgccggcgc gcgtgatgac gcgcaccgaa
     6361 atggtggtgt cttccgtcgg gcgaaactcg cccgatcccg ccacgacatt gcggtccggc
     6421 tggaagcctt cattattttc acgcacgatg acgaggtcaa catcgtcgaa cagcgcgccc
     6481 agccccggaa acgagcgcgt cgggcgcaca ttggcgaaca gcccgaaatg cttgcgcagg
     6541 atcgggtgcg ggttgatcgc gcccgccgcc ttcggatagg cgcggtggcc gatcggcccg
     6601 aggatgaaac catccagcgc cgacagcttt tccagcgtcc cttccggcat cgtcgtgccg
     6661 aggctgtcga gcgccgagcg gccgataggc atcgggcgcc aatcgatctc aaggccggcg
     6721 cgcgcagccg ccgccttggc tatgcgcacc gaagccggca cgatctcgtg cccgatatcg
     6781 tcgccattga ggatgcctac cgtcagatgg gccatgattg cgcctttcct gtcagccgcc
     6841 gaagatcgcc ggataccgtg tcagccggcc ttggccacca gcgccatcag gcggtcgagc
     6901 gaaggctggc tttcaaggtc gcgcaccagc gagacaacct cgtcagccgc cgcgcgggca
     6961 aggcgggcat gcgccgcatt atcccgaaac ttgtaggcga cctcctcata gctcatcgga
     7021 ttggccgggc tgcccttgcc gaaatcggcc tcgccgtgaa tgacgcggcc atcgtcgagg
     7081 gtcacgtcga tgatcgtcgt catcttgtgg tagccggcct tttccgcctt ttcgtcgatc
     7141 acgaagtcca ccttctcgat catcgccttg acgtcttcgc gctgcgtcgt cgcatcggtg
     7201 aactcgccaa ggcccgcctt gccgtcgagc agcaggatgg ccatgcagaa ttccatcgag
     7261 aacttggcct gcaattcgtc atgcgggcgg tgatggatga gcgcgttcgg catgttggag
     7321 ttggtgccga tgcggacatg cttgacgtcc ttcgccttga tgccgtgctc cttgatgagg
     7381 cgcagcatct ccgtcatgcc gggatgggta agcgagcctg acggatgcgg cttgatggaa
     7441 atgccgggat tggcgaaggt ccacggatcg cccagccgcc cgcgaatgga cgcagaatcg
     7501 taaccgccgc cggcggcgtt gaagaagccg cgcggcgctt cgagtatctt gtcggtcgcc
     7561 gtccagccga tggcggcgaa ttgggcggcg gcaacgccgc tttccgaaga gcgcccggca
     7621 tggaacggct tggtcatggt gccgaaattc tcgcgaaggc cggccgactg actgccggcg
     7681 atggaaagcg cgcgcgcggt cgtctcgaca tcgaagccga gcagcgaagc ggcagccgag
     7741 gcggcggcaa aggtgccgca ggtggcggta gcgtgaaaac cggtctggta atggcgcggc
     7801 gcgatggctt ccgcgatctt gcactcgacc tcgacgccca gatggtaggc cagcatcacc
     7861 ttggcgcccg aagcgttgag agcctcgccc atggcaagcg ccgccggcag cgccggtgcg
     7921 gtcggatgcg taagcaggcc atagacgcgg tccgtcgcca cagccagctg cgtgtcgtca
     7981 taatcgtcgg catggatgcc gacgccattg gcgaaagccg cgaagcgcgg cgcaaccttg
     8041 cggcccgcgc cgatgacggt cgaggaaccc ggtatcaggc cgatgtcgtc gaggtggcgg
     8101 cgcaccagcg ccccgctctg cgcgaccgag ccggacagcg ccaggccgat gccatcgagg
     8161 atcgatttct tgccaagcgc gacgacatct tccggcaggt cctgcggctt cgcatcaacg
     8221 acaaattcgg ccacatattt cgtcaggcct gcgtccacaa cgtcgtgcat cggtattcct
     8281 ctcgcttcaa atgccgcgcc ttcatgcggg gaacgccatt tgctcccgcc gaccggccaa
     8341 ctgtagggtc actaggaacc gaatttttgg taaactgtca acaatttttt gtcgacaaaa
     8401 gaggttatct cccacctttg gcaagccggt ggcgaggaga atttcagttc tgcaaggctg
     8461 gcttgggccg cgaggttgcg aaacacggcg cactttcacg tttcatggcc gaagctcgca
     8521 gcgccgcaca agaaacggcc gacaccggat cacgccagcg cgggacgacg ccatgcgcca
     8581 ggactattcg acgacatggc gcagcaccgc cgttgcgcgc cgcgtgcgcg tcgagccatt
     8641 gacgatatgc gccctgcccg ccgcaaaggc ccgctgcgga tcgcgcgcgg ccaccgcatc
     8701 cacgatggcg cgatgttcat gattggaaat gtcgagcaca tcgccgctcg ccagcgcgcg
     8761 gtagcgctgg atgtgaagct ctttcgccag accgagatag atctgcgcca ggcggctatt
     8821 gccgcacatc ctggccagct cggcgtggaa ttcgagattg actggataat actggctgac
     8881 ctcgcctgtc tttgccagat tatccatcct gtcgaccagc gcgcgcagcc tggtcacttc
     8941 cttgtcggtg atccgctcgg cgacgctcat cgtcatgcag gcgaatacgt aggcgcgcgc
     9001 ctcggcaacg tcgcgcgcct cctcttcgct gagttcgcgc acgaaaaagc cgcggttggg
     9061 aataacctgg ataagcccga ccgccgccag cgcgctgcac gcttcgcgaa tcggcccgcg
     9121 gctgaccttc agctgttgag caagcgcatt ttcgttgatt cgctcattgg gggtgaactc
     9181 cccctcgacg atcatccgct ctatctcatg ctggaccaca ttggccagcg aacgggtgcg
     9241 caggaagtcg atgggcgaga cgcccgacaa ttgttcgctt tgcttagcca tgggcagccg
     9301 gtccgcttgc actgatgttg gccttgcagc cgggcacgag ttgttttccc cgccacaaac
     9361 ggttcgcctg tcgatcagct actgcctctg gatcagccac tgcctctgaa tcgaccactg
     9421 cttgtctccc tcgccggact ggcagcgaca gccgccatat ccatgtcatg ccgggacgat
     9481 ataccccaat gtcaagcaaa acaccgacaa atactgtggg cttacacagc catttgccta
     9541 gccgccgctg cgctcgttca tccctccaag aaccggcacc cggttggcca ggaacactac
     9601 cgcgaacgac accaccagca acaggatgcc gagcaccgcg atggcgccgt agtcgccgct
     9661 ctcgttgagg tcgtagatga tgaccgaaac cagcttggtc ggcgccgtcg tcaacagcac
     9721 cgttgccgac aattcgcgga tcgtgccgat gaagacgaag gaccaggccg cgaccaggct
     9781 tgtcctgaga agaggcgccg tgatctgcca caggctttgc aggcgcgtgg cgccgaggat
     9841 gcggctggcc tcttccagtt ccggatgcac gccgaggaag gcggacgaca attgctggta
     9901 gccggccgga agctcgatcg tcatgaaggc gatcagcaga atccacagcg ttccatagag
     9961 gccgaatgtc ggattggcgt aggccaggaa cagcccgacg ccgagcacga tgcccggaat
    10021 ggcgatgggc gccgtcgcca gcatgccgag cagcggcgcg ccgcgcacag gccgccgggc
    10081 aaccagatag gcgatgacca gcgccatgcc tgtggcgatg gtggcggtcg acaggccgag
    10141 gatcagcgtg ttccagatgg ccagccgcgt gctcgaaaac tcgaacagca cgaaatggaa
    10201 attatgcagc gtcagcgaag accagctcag cggctccgaa atattgtgcg tcaacgccac
    10261 cttgagcagc gcgaggtagg ggaacacgat cgttatgcac aagatgaaca gcacataagc
    10321 gccggcaagc catttccatg ggccaagctg taccttgcgt ggggtgccgc ttttgccgcc
    10381 aagcaccgta tagccgcgac ggccaagtag ccacttctgc ccccacagca acagcatggt
    10441 cacgatcagc agcggaacgg cgctggccgc cgccaggtcc aggcgcggcg ggaactggaa
    10501 atagctccag atcttggtgg tgatgacatg gaagcccgca ggcagcgcca ggatggcggg
    10561 ggaaccgaac atggtgaagg cctgcacgat ggccaccagc gaaccggcaa gcatcgccgg
    10621 cagcaccatc ggcagcgtca cccgccgcag cgtcgttccc ggcttgccgc caagaatggc
    10681 ggaggcgtcc tcaaggtccg aaggcacctt gtcgaacgca ttggccacca gcgtgaacac
    10741 gaagggaaac gaatagcagg tgatcgcgaa aaccagcccg gtgaaggtgt agatgttgaa
    10801 caggctctgc gattggtcga gaccgaatgc ccagcgatag atgttgttga ggatgccgct
    10861 gttgggcgcg gccagcatca cccaggcgat ggcgccaagg aagggcggtg tgacgaagga
    10921 agcggtcacc aggctgcgca ccagcctttg tccgggcatg tcggtacgcg cgaccagcca
    10981 ggccagcggc gttgcgacga ggcaggagaa aaagccgacg cagcccgcca tggcgagcga
    11041 aatgaggaac gcccgccgga tggtggcgtc ggtcagcagc ttgacgaaat tagccagcgt
    11101 cggcgaaccc gacttgtcgg tgaaagcata aaaaccaagc cagaacagcg gcaacagcac
    11161 cagcacgcaa agaacaaccg ccagcagcac gaacagcggc gtcgacaggt cgagcttgaa
    11221 acgacgccgt ccgagggacg gatcggggga gaccgcagcg attgtcacct ttgtcaccta
    11281 tcagcttgat tggtatcgca aacctgctgg cgaggcaggc acgcatcttc gttcgaacgc
    11341 cgccattcct cgcccgcgat cgaaaacccg gcatttggcg caggcctcta tagattcgac
    11401 gcgcagcacg gcttgatccg gcgggaaggc ggtggcgcca aacgcgccat cgccttgccc
    11461 gtgtccgccc gacagccgct accggcaatc gggcgggagc gtttatcagg tgccgaaata
    11521 ctcttcgtac ttctgcttga tctcttccat cgcgtcacgc accttggccg gatcggaatc
    11581 gagcagcttg atctgcgaga gcggcgtgcg gttggacttc tcctttacgt ccgggcgaac
    11641 cgagcgcaac ccgccgaaat cgtcgttcaa ctgctgggct tcctgcgtga acatgaagga
    11701 atagaacagc ttggccgcat tcggatgtgg cgcgtaggcc agcaccgccg cgttgccgta
    11761 ggcgatggag gtgccttccg gcggatagac ggccttgatc ggcacgccct tatcctggag
    11821 gatgaagacg ttgtactcgt tgccgtccgc ctggatgttg cgctcgccct gcgccagaac
    11881 cttcggcggc tcggtcgaag actgcacctg catcacgccc tgcttgccga gcttctcgaa
    11941 gaagtcccag cccagaagct ggctcagcgc ataggtcgcc gtcatgacgt tgccgctgta
    12001 gccgggatgc gacttgacca gcttgtcctt gtatttcgga tcgagcagat cgttccacgt
    12061 ctttggcgca tcctcttcct tcaccttgtc ggtgcggtag gcgatgaccg tcagcgtggc
    12121 gcggaatgcg gcatacatgc cgtccgggtc cttgtccttt tccggccaca tcttggcgac
    12181 atcttccggc accagcggcg tcagccagtt ctggtccttg aacgtcagga agtgtgaggc
    12241 atcggatgtc tcgatgacgt ccgcattatg gatgccgctc gaatattcct gcccgatgcg
    12301 ctggaagacg cgttccgaac cggcgcgctc gacctgcacc ttgatgcccg gataatgctt
    12361 ttcgaatgcg gcgcccaact tctccgccac ctgcacgtcg gtcgacgtgt agtagacgac
    12421 ggaggcctcc ttggtggccg ccgcgaccag gtcgtcggtc acggtgtagg gttcgggcgg
    12481 cgcggcccag gccacctggc tccacgccaa ggcggcgaaa agcgcgccgg ctgcgacttt
    12541 actgttcgac atttctctct cccttaatgt gcgagcgccc ggcagcgatc aggtgggcaa
    12601 tgaagccaaa cttgctggtt ctccgggatc gccacgccta cgggcgcaat cgcccgtacc
    12661 gtttcgccac ctgatagact gacaaggtag tcgcggtgcg aaccgagata gacctgccgc
    12721 acgacctttc cggtcgccca gttttccaga tcgccactcg gcttttcgcc cacgagatgc
    12781 aggtcgtgat gccgcaccga tacggcggtc ggcccattgg cggcaaagtc gccgcttccg
    12841 catttgagga tgaggccgtt gccgacatcg acggcgttgt cgccgatgcg atttcccttg
    12901 aagatattgg tgccgccgat gaactgcgcc acgaattccg attgcggccg ctcatagatg
    12961 tcctcgggag aaccggcctg ctcgacattg ccctggttca tcaccacgat gatgtcggcg
    13021 gcggtcatcg cctcggtctg gtcatgcgtg acatagaccg tggtgtagcg gaattcgtcg
    13081 tggaggcggc ggatttcaaa gcgcatttcc tcgcgcagat tggcgtcgag gttggaaagc
    13141 ggttcgtcga gcaacagcgt ttccggctcg atcaccagcg ccctggccag cgaaacgcgc
    13201 tgctgctggc cgcccgacag ttcccccgga tagcgttccg ccagcccgtc aagcttggtg
    13261 gcctgaagga tgcgcttggt cttggcctgg atttcgctct tggccatttt gcgcagcctg
    13321 aggccatagg cgacgttctc gaacaccgtc atgtgcggcc acagcgcgta gctctggaag
    13381 atcatcgaca tgttgcgcgc ttccggcggc tcactggcgc tggccgacga gacgacacgg
    13441 ccgccgacgc ggatttgccc gtccgaaggc tgaacaaaac cggcgaccag ccgcaaggtc
    13501 gtggtcttgc cgcaacccga cggcccgagc aggcacacca gcgtgccctt ctcgatggcc
    13561 agatcaaccc cgttgacgac cgtgaccttg ccgtagcgct tggtcaactt gtcgagttcc
    13621 acaaaggcca tggatcctcc cagcaatcct tgcaggtaag gcacctgcat ctctttgctc
    13681 agggtcagtc caattttgtt aattgtcaac aattaactga cgactattcg cattatatgc
    13741 ccgagtgccg gtcaggccct cggcaggccg ccaatgaagg cgctattgca gcgccagcaa
    13801 accgcagaag ataattgcac ccgcgcccat cagcgtcagc gggccaagcc ggaccttccg
    13861 ggaggcgacc gcgaccagta tcgccacgaa ccaggcgatc cacccgcccc tcgcttcttg
    13921 cagcagcgcg aacacgctcg acagaaccag ccccgccgcc agcggcgcca gcccatagat
    13981 aagcgccttt tgccatgccg cgttcggatg gcggtgccag aaataggcga ccaccacgac
    14041 cagcatggaa gacggcgcaa agatcgcgat ggtggaaacg atcgccccaa gcaggccagc
    14101 cagatgccac ccgatcagcg tgacgatcag ggttcccggc cccggcgcta cccgcgaaat
    14161 ggcgaacatg tccagaaaac cctgattggt cagccaatgc tggttgtcga ccacgctgcg
    14221 ctgcaattcg cccagcacgg aagcgccgcc gccgatcgcc atcagcgaca gcggcgcaaa
    14281 gaccagcagc atggtgagga gaagattgtc gtccttcatg accggttcct caggaagccg
    14341 atgccgatgc cgatgggcgc aagcaccgcg accacgacga gggctgaaaa atgaaaggcc
    14401 gtcgcgccga tgaatgtgcc gacgaacagg aggacggagg caatgtcggt gcaggcatag
    14461 cgcgtggcga ggtagccgac cgacagcgac agcccggttg cagcgacagc cagcccggca
    14521 agcagggaac ctatctcggg aacattcgtc atcctcaata ccacgtcgcc gataaggatg
    14581 atcacgaagg cgggcggcaa aaggacggct gccagcgcga ccaccgcgcc gatgatgccg
    14641 cggcattgat agcccagcca aagcgccagg ttcgccacat tgacccccgg caaggactgg
    14701 ctgagcgcga ggccggacag aaattgctca tccgtgaacc agcgccggtg gatgacgaat
    14761 tcgctgtgca tcatgccgtt gacgccgccg ccgatgctgg tgatgccaag cttggtgaac
    14821 accaggaaaa gttgaagaag gcccggcttc ggcaaggccg gctcctcggg cttcggcgag
    14881 gccggcttca atgcgggaag atcggcttga ggcatgaatg ctccacttct tgtcggcgcc
    14941 gggcgctttg ccttgcccgc cgccatgcat ccagaggaac tgcgtagcta tcggcaatcc
    15001 ggcccttgcg ggcaaagttg ccaggcgacg catcgcaccc gctctggcat gcctcggccg
    15061 ccattccaag agggtggcgg cgcacgccat atgttagttt tacaaacaag ctcgcctcct
    15121 tactgctgac tgtattctgt tgacagtttt cagtaaatag tggatcaccg acgtatgaga
    15181 tcggaactcg aatttcttca aaagcactcc ctgacaagcg ttctccagcg cgaattggaa
    15241 accttgattt tgtcgggtga gttttcgccg ggcgaacgtc tgaacgagaa cgccctggcc
    15301 gcccgctttt ccgtcagccg cgggccggtg cgcgaggttt gccgcgcgct tgccgaaaag
    15361 gggctgcttg aactgatccc caatcgaggc gtgttcatgc gccgcataga cgaggccgag
    15421 gccgccgaac tctacgatct gcg
//
LOCUS       DASYCS010000173         7510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3230485,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000173 DASYCS010000000
VERSION     DASYCS010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 7510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7510
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3230485"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="VGM46_05590"
     CDS             complement(<1..564)
                     /locus_tag="VGM46_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008935820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HEY4192097.1"
                     /translation="MSEVSLAFPDGSVRVYDAATTGAQLAESISKSLAKKAVAYAMDG
                     TLRDLSDPLGASGQVEIVTRDDPRALELIRHDAAHVLAEAVQELWPGTQVTIGPVIEN
                     GFYYDFARNEPFSPDDFPIIEKKMREIIGRNKPFTKEVWSREKARKVFSDKGEAYKLE
                     LIDAIPEDQDLKIYAQGDWFDLCRGPHM"
     gene            complement(678..1052)
                     /gene="yidD"
                     /locus_tag="VGM46_05595"
     CDS             complement(678..1052)
                     /gene="yidD"
                     /locus_tag="VGM46_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008838114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEY4192098.1"
                     /translation="MCKDHSVPLSKRGAPAGRNWAGPWRKTPGRLVGTSMVRLYQLTL
                     SGFVGNSCRHLPTCSEYAHEAIARHGLWAGGWMGLFRVMRCGPGGTHGIDRVPEVLEP
                     RYRWFTPWRYWLVGKKSHGAGN"
     gene            1283..2314
                     /locus_tag="VGM46_05600"
     CDS             1283..2314
                     /locus_tag="VGM46_05600"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY4192099.1"
                     /translation="MSDHVELGLDTFGDVTFGPDGKMLSQAEVIRNVVAQAVLADETG
                     VDFIGVGEHHRDDFAVSAPETVLAGIAGKTNRIRLGSAVTVLSSDDPIRVFQRFSTLD
                     ALSNGRAEVILGRGSFTESFPLFGFRLNDYEKLFSEKLDLFAALLKQEPVTWEGTTRP
                     PLSNQSVYPPIENGTLKAWIGVGGSPESVIRAARYRLPLMLAIIGGDPKRFRPYVDLY
                     HDALKQLEAPSLPIGVHSPGHIADTDEQAREELWPSYKAMHDRIGKERGWGPTTPANF
                     NSEADMGSLYVGSPETVARKIAQTVKALGLARFDMKYSSGPLPHEKLMRSIELYGARV
                     IPMVRDLLA"
     gene            complement(2370..2816)
                     /locus_tag="VGM46_05605"
     CDS             complement(2370..2816)
                     /locus_tag="VGM46_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HEY4192100.1"
                     /translation="MIDDVYNAKILSFAGNIGRIGRLERPDATAKAHSKLCGSTVTVD
                     LAVDNGVVTDFAHDVKACALGQASSSIMAEHVVGASAEELRAVRETMLKMLKENGPPP
                     EGRFADLKYLEPVRDYKARHASTLLTFDAVVDALDQIEKKDAERAA"
     gene            3049..3678
                     /gene="folE"
                     /locus_tag="VGM46_05610"
     CDS             3049..3678
                     /gene="folE"
                     /locus_tag="VGM46_05610"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006335060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEY4192101.1"
                     /translation="MDVIINKPRPDAAYMEKPITDRPSQAEVEAAVRVLLRWTGDDPG
                     REGLIDTPKRVAKAYREMFGGYDMCPADELGRTFEEVAGYDDLVIVKDIQFHSHCEHH
                     MVPIIGKAHVGYLPDGKVVGLSKIIRVVEIFAHRLQTQEAMTAQIAGVIQDVLNPRGV
                     AVMLEAEHMCIAMRGIRKQGSTTLTSTFTGVFKDNPEEQARFISMVRGG"
     gene            3707..4165
                     /gene="hisI"
                     /locus_tag="VGM46_05615"
     CDS             3707..4165
                     /gene="hisI"
                     /locus_tag="VGM46_05615"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008838119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEY4192102.1"
                     /translation="MSALEFPSPSADKKVLEEGAVFSPRFDAGGLVTAVVTDADDGMV
                     LMVAHMNAQALALTLETKVAHYWSRSRSALWKKGETSGNVQQVIEIRTDCDQDAVWLR
                     VKVSGHDATCHTGRRSCFYRMVGLHDGAGTLAISDGQVLFDTEKTYRKSP"
     gene            4247..5218
                     /locus_tag="VGM46_05620"
     CDS             4247..5218
                     /locus_tag="VGM46_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015317575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HEY4192103.1"
                     /translation="MLDWASLRGRSDLREANGPSSPDGASDGKPAKQTGIALALGGGC
                     ARGWSHIGVLRALDEAGIEVSMIAGTSIGALVGGCYLAGKLDQLEEFARSLTKRRIFG
                     LLDLNLRGNGLFGGMKLDARLREHLNGIRIEDLPKPFVCVAAEIRTGHEIWLSSGSLV
                     TAMRASYALPGVFEPVTCNGRVLVDGALVNPVPVSICRAYEQPLVVAVNLSHDNFGRS
                     AVIRHSAGELLVQHDVPAPPHHQSDAQAKESRLGITGVMVEAFNIIQDRISRARLAGD
                     PPDMSLHPKLGHIGMTEFYRADEAIRLGYEVTIGQLEELKRLQAVLV"
     gene            complement(5296..7083)
                     /gene="recJ"
                     /locus_tag="VGM46_05625"
     CDS             complement(5296..7083)
                     /gene="recJ"
                     /locus_tag="VGM46_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEY4192104.1"
                     /translation="MSGEKRYFLNVRRSATGVSWEHRLNERQDMVALAIAQGEGMPDI
                     VARVLAGRGVTAAEAARFLDPTIRALLPDPASLTDMERAASRIADAVIGREKVAIFGD
                     YDVDGAASSALLKRFLDHFDVPSEIYIPDRIFEGYGPNPEAMRELVSRGASLIVTVDC
                     GTNSAVSIDAAKAVGADVVVLDHHQVGGPLPSAASAIVNPNREDDLSGQGHLCAAGVV
                     FLTLVQTAKVLRGRLPGKAQPDLLGLLDLVALATVCDVVPLIGVNRAFVVKGLQVVRQ
                     QKNVGLVALARVARIGEPVAPFHLAFLIGPRINAGGRIGDAALGSRLLATDDPVEART
                     IAETLDRLNQERQAMEQEMLAQARAEADAELAGGNGPAVIVTASDTWHPGIVGLLASR
                     LKDHARRPAFAIAFNSSDIGTGSGRSVAGFDLGRLVRSAAEAGLIVKGGGHAMAAGIT
                     VEREKLGELRAFFEERAATDVFRLQDEEALHIDAALAAEGATLDLLDGLEKAGPFGAG
                     HVAPIFALPRHRIADARLIGTNHIRVDLQSESGGRVQAVAFRAADTPLGDFLFKSRGQ
                     TIHIAGSLSGNYWNGNRTVQFRIIDAAPA"
     gene            complement(7234..>7510)
                     /locus_tag="VGM46_05630"
     CDS             complement(7234..>7510)
                     /locus_tag="VGM46_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192105.1"
                     /translation="AELRREMNRMNKTMAERAEDVSEQASGWYDSASEGASRAARQLR
                     TRAQSVSETVQQNPGTVSSALALGGVIGFMLGILVVQSSSSNGRRWY"
BASE COUNT         1416 a         2408 c         2262 g         1424 t
ORIGIN      
        1 catgtgcggc ccgcggcaga ggtcgaacca gtcgccctgg gcatagatct tgagatcctg
       61 gtcctcggga atggcgtcga tcaattccaa cttgtaagcc tcgcccttgt cggaaaatac
      121 cttcctggct ttctcgcgcg accacacttc cttggtgaac ggcttgttgc ggccgatgat
      181 ttcacgcatc ttcttttcga tgattggaaa atcatcgggc gagaaaggtt cgttgcgggc
      241 gaaatcataa taaaacccgt tctcgatcac cggtccgatg gtaacctgcg ttcccggcca
      301 aagctcctgc accgcctcgg caagcacatg cgccgcatcg tggcggataa gctccaacgc
      361 gcgcgggtcg tcgcgcgtca cgatctcgac ctggccggac gcgccgagcg gatcggaaag
      421 atcgcgcagc gtgccgtcca tcgcataggc gacggctttc ttggccaagg atttcgaaat
      481 ggattcagcc agttgcgcac ccgtcgtcgc cgcatcgtag acgcggaccg aaccatcggg
      541 aaatgcaagg gaaacttcgg acatcggttt tctcctatcc agtcccgcaa acgagcgcgg
      601 gtggcgatat gccggatgcg tccggcgacg agcggtgttt tagtcagcca gttcgggcgc
      661 gtaaaggcgc gatccagtca gtttccggcg ccgtgcgact tttttccgac aagccagtac
      721 cgccacggcg taaaccagcg atagcgaggc tccagcacct ccggcacgcg gtcgatgccg
      781 tgggtgccgc ccggcccgca gcgcatgacg cgaaagagtc ccatccagcc gcctgcccaa
      841 aggccgtggc gggcgatcgc ttcatgcgca tattccgagc aggtcggcaa atgccggcat
      901 gaattgccga cgaaccccga aagcgtcagc tggtagaggc gcaccatcga cgtgccgacc
      961 aaccggccgg gcgtcttgcg ccatgggccg gcccagttgc ggccggccgg tgcgccgcgt
     1021 ttcgacagcg gcacggaatg gtccttacac acgcttttcg cctcaaattc tttatgtaac
     1081 cgcaaggctt caccgtcaaa cgagggtgaa cagtctgtgc accctcgtca attaggtcgg
     1141 cggctttgaa cccctatcta atctttcaac ttgcctgcat ccagttttcg catctatcga
     1201 agcgttcttt tcgggcgcag gcatttcgca acagtgtcgc aaagccgcag ttggccggcc
     1261 ggcgaacaaa ggaaacaata gcatgtcgga ccatgtcgaa ctcggactgg atacatttgg
     1321 cgacgttaca ttcggcccgg atggcaaaat gctttcgcag gctgaggtta ttcgaaatgt
     1381 cgtcgcccaa gccgtgctgg ccgacgaaac cggcgtcgat ttcatcggcg tcggcgagca
     1441 tcaccgcgac gatttcgcgg tttcggcccc tgaaaccgtg ctcgccggaa ttgccggcaa
     1501 gaccaacagg atacgtctgg gttcggctgt caccgtctta agctcggacg accccatccg
     1561 ggtctttcag cgtttctcga ccctggacgc tttgtcgaac ggccgtgccg aagtcattct
     1621 cggcagaggg tcgtttactg aatcctttcc gctgttcggc ttccggctca acgactacga
     1681 aaaactgttt tccgaaaagc tcgacctgtt tgcagccttg ctgaagcagg agccggtcac
     1741 ctgggaaggc acgacgcgcc cgccgctttc caaccaaagc gtctatccgc cgattgaaaa
     1801 tggcacgctg aaggcctgga tcggcgttgg cggcagccct gaatcggtca tccgcgcagc
     1861 ccgttacagg ctaccgctca tgctcgccat catcggcggc gatcccaagc ggttcaggcc
     1921 ctacgtcgat ctttatcacg acgcgctgaa acagctggaa gcgccgtccc tgcccatcgg
     1981 cgtccattcc cccggccaca tcgccgacac cgacgagcag gcgcgcgagg aattgtggcc
     2041 gagctacaag gcgatgcatg accgcatcgg caaggagcgc ggctggggac cgacgacgcc
     2101 cgccaatttc aactccgagg ccgacatggg ttcgctctat gttggctcgc ccgaaaccgt
     2161 cgcccgcaag atcgcccaga cggtcaaggc gctcggcctg gcgcgcttcg acatgaaata
     2221 ttcctccggc ccgctgccgc atgaaaaact gatgcgcagc atcgagcttt acggcgccag
     2281 ggtcattccc atggtccgcg acctgttggc ctgaaacgcc cggacccatg ggcagcttac
     2341 attttgcttg ctgcccatac gttcggcgct caggccgccc gttcggcgtc tttcttctcg
     2401 atctggtcga gcgcgtcgac tacggcgtcg aaggtcagca aggtggaggc atggcgcgcc
     2461 ttgtagtcgc gtaccggctc cagatatttg aggtcggcga accgaccctc gggcggcggc
     2521 ccgttttcct tcagcatctt cagcatggtt tcgcgcaccg cgcgcagttc ttccgcgctc
     2581 gcgcccacca catgctcggc catgatggag gacgatgcct ggccgagcgc gcaggctttc
     2641 acatcatggg cgaaatcggt gacgacgcca ttatccaccg caagatcgac ggtaacggtg
     2701 gacccacata attttgaatg tgccttggcc gtcgcatcgg ggcgttcgag ccggcctatc
     2761 cgcccgatat tgccggcaaa actcagaatt ttcgcgttat agacatcgtc gatcatgatt
     2821 ttccgctttc tcgccgcccg gcgaacagcg attgccgctt gtgtatcttt tattcgcaat
     2881 ggcaaatcat atatagtgtt ggtgttcctg gaacggcaag gcgacttgca catcgatgtt
     2941 ccgggaacca tgtcacgccg ctttcgggcc gtattcaggc gatccgcgaa aggctgtggt
     3001 ccgtttaact cgtccctcct gtgaggggga ctacgggaga cgccttctat ggacgttatc
     3061 atcaacaagc cccggcccga tgccgcctat atggaaaagc cgataaccga ccgcccaagc
     3121 caggccgagg tcgaagcggc ggtgcgcgtg cttctgcgct ggaccggcga cgatccgggc
     3181 cgcgagggcc tgatcgacac gccaaagcgc gtggccaagg cctatcgcga aatgttcggc
     3241 ggctacgaca tgtgcccggc cgacgagctt ggccgcacct tcgaggaggt tgccggctat
     3301 gacgacctcg tcatcgtcaa ggacattcag tttcattcgc attgcgagca ccacatggtg
     3361 ccgatcatcg gcaaggcgca tgtcggctac ctgcccgatg gcaaggtggt cggcctgtcc
     3421 aagattatcc gcgtcgtcga aatcttcgcc catcgcctgc aaacgcagga ggcgatgacc
     3481 gcgcagatcg ccggcgtcat ccaggacgtt ctcaacccgc gtggtgtcgc cgtgatgctg
     3541 gaggccgagc atatgtgcat cgccatgcgc ggcattcgca agcagggatc gacgacgctg
     3601 acctccacct tcaccggggt cttcaaggat aatccggaag aacaggcgcg cttcatcagc
     3661 atggttcgcg gcggctaggc acggccgcgc aagacaagga ccggcaatgt cggcgttaga
     3721 gtttcccagc ccatcggccg acaagaaggt tctggaagaa ggcgccgtct tctcgccgcg
     3781 cttcgacgcc ggcgggctgg tcaccgccgt cgtcaccgat gccgacgacg gcatggttct
     3841 gatggtcgcc catatgaatg cgcaggcatt ggcgctgacg cttgaaacaa aggtcgctca
     3901 ttactggtcg cgctcgcgtt cggccctttg gaagaagggc gagacctcgg gcaatgtgca
     3961 gcaggtcatc gaaatccgca ccgactgcga tcaggacgcc gtgtggcttc gcgtgaaagt
     4021 gtcaggccac gacgcaacct gtcacaccgg ccggcgttcc tgcttctatc ggatggtggg
     4081 cttgcatgat ggcgcgggga cgcttgccat cagtgacggc caggtgctgt tcgatacgga
     4141 aaagacgtat cgcaagtcac cataacgggt cgattgtccc ggaccttaat tcatgatttc
     4201 gatacgggac gaatgctagg atgattgcgg gacaagggag atcgtgatgc tcgactgggc
     4261 gtcattgcgc ggcagatcgg acctgcggga ggctaacggc ccctcgtccc cggatggtgc
     4321 cagcgacggc aagcctgcga aacagaccgg catcgcactg gcgctgggcg gcggatgcgc
     4381 gcgcggctgg tcgcatatcg gcgtgcttcg cgccttggac gaagccggca tcgaagtatc
     4441 gatgatcgcg ggaacttcca tcggcgcgct ggttggcggc tgttatctcg ccggcaagct
     4501 tgaccagctt gaggaatttg cccgcagcct caccaagcgt cgcatcttcg gcctgctcga
     4561 cctcaatctg cgcggcaacg gccttttcgg cggaatgaag ctggatgcgc ggctgcgcga
     4621 gcatttgaac ggcatccgca tcgaggattt gcccaaaccc ttcgtttgcg tcgccgctga
     4681 aatccgcacc ggccatgaga tctggttgtc cagcggttcg ctggtcaccg ccatgcgggc
     4741 gtcttacgcg ctccccggcg tcttcgagcc ggtaacctgt aacggccgcg tcctggtcga
     4801 cggcgcgctg gtgaacccgg ttcccgtctc gatctgccgc gcttatgaac agccgctggt
     4861 cgtggcggtc aatctcagcc acgacaattt cggccgctcg gccgttatcc gccacagcgc
     4921 cggcgaattg ctggtgcagc atgatgtgcc cgcccccccg catcatcaaa gcgatgcgca
     4981 ggccaaggaa agccgcctcg gcattaccgg cgtcatggtg gaagccttca acatcattca
     5041 ggaccgcatt tcgcgtgcgc ggctggcggg cgacccgccg gacatgtccc tgcaccccaa
     5101 gcttggccat atcggcatga ccgaattcta ccgcgccgat gaagccatcc gcctcggcta
     5161 tgaagtcacc atcggccagt tggaagaact gaagcggctg caagcggttc tggtttaacg
     5221 aaacccctgg caaaacggcg ccggtttttc cattcggatc gcgcgagacg acgcaaccgc
     5281 aagaagcgga cagcgctagg cgggtgcggc gtcgataatc ctgaactgca ctgtcctgtt
     5341 gccgttccag taattgcccg acagcgaacc ggcgatatgg atggtctggc cccggctttt
     5401 gaacaggaag tcgcccagcg gcgtgtccgc agcccgaaag gcgaccgcct gcacccgccc
     5461 gccgctctcc gattgcagat cgacgcggat gtgatttgtc ccgatcagcc tggcatcggc
     5521 gatgcgatgg cgcggcagag caaatatcgg cgcgacatgc cctgccccga agggcccggc
     5581 cttttcaagc ccatccagca gatcgagcgt cgcgccctcc gcggcaagcg ctgcatctat
     5641 atgcagcgcc tcctcgtctt gaaggcgaaa cacgtcggtg gcggcccgct cctcgaagaa
     5701 tgccctaagc tctcccagct tttcccgctc caccgtgatg ccggctgcca tcgcatggcc
     5761 gccgcccttg acgatcagcc cggcttcggc ggcggaacgc accagccggc caaggtcgaa
     5821 accggcaacc gagcgccccg aaccggtgcc aatgtcggaa gaattgaagg cgatggcaaa
     5881 ggcaggccgc cgcgcatgat ccttgagccg cgaggcaagc agcccgacaa tgccgggatg
     5941 ccaagtgtcg ctggccgtga cgatgacggc cgggccgttg ccgccggcca gttccgcatc
     6001 ggcttccgcg cgggcctgcg ccagcatctc ctgctccatc gcctgccgct cctgattgag
     6061 ccggtcgagc gtttcggcaa tggtgcgcgc ctcaacggga tcgtcggtag caagcaggcg
     6121 actgccgagc gcggcatcgc cgatgcgccc gccggcattg atgcgcgggc cgatcaggaa
     6181 ggcgaggtga aacggcgcga ccggctcgcc gatacgcgcc acccgcgcca gcgccaccag
     6241 cccgacattc ttttgctgcc gaacgacctg caagcctttg acgacgaagg cgcggttcac
     6301 gccgatgaga ggcaccacgt cgcagacggt cgccagcgcc accagatcga gcaggccgag
     6361 cagatccggc tgggcttttc ccggcaacct gccgcgcagg actttcgccg tctgcaccaa
     6421 tgtcaggaaa accaccccgg cagcgcataa atgcccttgg ccggagagat cgtcctcgcg
     6481 gttcggattg acgatggccg acgccgccga tggcagcggc ccgccaacct gatgatggtc
     6541 gagcacgacg acatcggcgc cgacagcctt tgccgcatcg atggaaacgg cgctgttggt
     6601 gccgcaatcg acggtgacga taaggctggc cccgcgtgaa acaagctctc gcatcgcctc
     6661 cgggttaggc ccgtaaccct cgaaaatgcg gtcgggaatg tagatttcgc ttggtacgtc
     6721 gaaatggtcg agaaatcgct tgagaagcgc cgacgaagcc gcgccatcga catcgtaatc
     6781 gccgaaaatc gctacctttt cacgcccgat caccgcatcc gcgatacgcg atgcggctct
     6841 ttccatgtcg gtcagggatg ccgggtcggg cagaagcgcg cgtatcgtcg gatcgaggaa
     6901 ccgtgccgcc tcggcagccg tgaccccgcg cccggccagc acccgcgcca cgatatccgg
     6961 catgccttcc ccctgggcga tggcaagcgc caccatgtcc tgccgctcgt tcagccgatg
     7021 ttcccaagaa acaccggttg ccgagcgcct gacattgagg aaatagcgtt tttcgccgct
     7081 catgcggcgc atccgtgaat cgtcatgccc tactttaggc gacgccacgc cgcgacggta
     7141 tcggaatctg gccgaccccg ccgtttggcg gcggtcctat ggtggaaatg acggcgcgat
     7201 aaagcgccgt catttccaca ccgaagccgg cgatcagtac cagcggcggc cgttggaact
     7261 ggaggattgg acgaccagta tcccgagcat gaaaccgatc acgcccccca gcgccaatgc
     7321 actggagacg gtgccgggat tttgctgcac ggtttcggag accgattggg ctctcgtccg
     7381 caactgcctg gcggcgcgcg atgcgccttc ggatgcgctg tcgtaccaac cggaggcctg
     7441 ttcggaaaca tcctcggccc gctccgccat cgtcttgttc atcctgttca tttcgcggcg
     7501 cagttcggcg
//
LOCUS       DASYCS010000174         2338 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_5115732,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000174 DASYCS010000000
VERSION     DASYCS010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5115732"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="VGM46_05635"
     CDS             complement(<1..721)
                     /locus_tag="VGM46_05635"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012090853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEY4192106.1"
                     /translation="MAVYTDISEVELAGFLDGYPIGELLSYKGIAEGVENSNFLLHTS
                     KGAYILTLYEKRVNRDDLPFFIGLMQHLNDKGINCPLPVQRKDGRIIGELAGRPAAIV
                     TFLEGIWTRRPTAEHCREVGKSLAAMHLAGQDFSLRRTNALSVDGWRPLWDRAAARAD
                     EVEPGLAREVEADLAALEKEWPADLPKGVIHADLFPDNVFFLGKNLSGLIDFYFACWD
                     MLAYDIAICLNAWCFEKDNSFN"
     gene            complement(721..1725)
                     /gene="ispH"
                     /locus_tag="VGM46_05640"
     CDS             complement(721..1725)
                     /gene="ispH"
                     /locus_tag="VGM46_05640"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010914897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEY4192107.1"
                     /translation="MLQTKPPLTIRLCQPRGFCAGVDRAIQIVVLALKKYGRPVYVRH
                     EIVHNRYVVEGLQNLGAVFIEELSEIPDEHRESPVVFSAHGVPKSVPADAVARNLFYL
                     DATCPLVSKVHKQAMRHQRQGRHVLLIGHAGHPEVIGTMGQLPEGAVTLIETEADAAT
                     FTPADPATLGFVTQTTLSVEDTAGIIRALRQRFPHLQAPAAESICYATTNRQEAVKET
                     AAGADLYLIVGAPNSSNSRRLVEVAERAGAKMSLLVQRASEIPWNDIGGIATLGLSAG
                     ASAPEIIVDEIIEAFRARFDVSVDLAITATETEDFPVMRVLRDVELTPADMAFVNGNA
                     "
     gene            complement(1805..>2338)
                     /locus_tag="VGM46_05645"
     CDS             complement(1805..>2338)
                     /locus_tag="VGM46_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019856996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HEY4192108.1"
                     /translation="VDYMPVTVTGTFVHEAERHFFATWDGASGFDVYTPLHLDDGRYL
                     LVNRGFVPYELKDAAKRPQGQVAGKVTITGLARNPLFGKPSSMVPDNDPVKNIFYWKD
                     RDVMAATAGLPAGYRLAPIFIDANNAANPGGLPIGGVTLIDLPNNHLQYIFTWYGLAA
                     ALVGVMFFWLRRPAKPQ"
BASE COUNT          441 a          701 c          772 g          424 t
ORIGIN      
        1 ggttgaagga attgtccttc tcgaagcacc atgcgttgag gcagatggcg atgtcgtagg
       61 ccagcatgtc ccaacaggcg aagtagaaat cgatcaaccc ggacagattc ttgccgagaa
      121 aaaagacgtt atcgggaaac aggtcggcat gaatgacgcc tttcggcagg tcggccggcc
      181 attctttttc gagtgcggcg agatcggcct cgacctcgcg cgccaagccc ggttccacct
      241 cgtcggcgcg cgccgccgcc cggtcccata gcggccgcca cccgtccacc gagagcgcgt
      301 tggtgcgccg cagggaaaaa tcctggcccg ccagatgcat ggccgccagc gactttccca
      361 cttcgcggca atgctcggcc gttggccggc gagtccatat gccttcgaga aaggtaacga
      421 ttgccgccgg gcggccggcc agttcgccga tgatccggcc atccttgcgc tgcaccggca
      481 acgggcagtt gatgcccttg tcgttcaggt gctgcatcag gccgataaag aagggcaggt
      541 cgtcgcggtt cacccgcttt tcgtagaggg tgaggatata ggcccccttc gaggtgtgca
      601 gcaggaagtt ggaattctcc accccttcgg cgatgccctt gtaggaaagc agttcgccga
      661 tcgggtaacc gtcgaggaac ccggccagct cgacttcgga aatatcggtg tagacggcca
      721 tcaggcgttt ccgttgacga atgccatgtc ggccggtgtc agttccacgt cgcgcagcac
      781 ccgcatgacg gggaaatcct cggtttcggt ggcggtgatc gcaagatcga cggaaacatc
      841 gaagcgggcg cggaaggcct cgatgatctc gtcgacgatg atctctggcg ccgatgcccc
      901 cgccgaaagg cctagtgtgg caatgccgcc aatgtcattc cacggaattt ccgaagcgcg
      961 ttgtaccagc agcgacattt tcgcgccggc gcgctcggcc acctcgacga ggcgccgcga
     1021 attggacgag ttcggcgcgc cgacgatgag gtacaaatcc gcgccggcgg cggtttcctt
     1081 taccgcctcc tgccggttgg tggtggcata gcagatcgat tccgccgcgg gcgcctgcaa
     1141 atgcgggaac cgctgccgaa gcgcgcggat gatgccggcg gtatcctcga ccgacagcgt
     1201 ggtctgcgtg acgaagccca gcgtcgctgg gtcggcaggc gtgaaggtgg ccgcatccgc
     1261 ctcggtctcg atcagcgtca ccgcgccttc gggcaactgg cccatcgtgc cgatcacttc
     1321 ggggtggccg gcatggccga tgagcaggac atggcggccc tgccgctgat ggcgcatggc
     1381 ctgcttgtgg accttggaga ccagcgggca ggtggcgtcc agatagaaaa gattgcgcgc
     1441 gaccgcatcg gccggcacgg atttcggcac gccatgcgcg gaaaacacca ccgggctttc
     1501 gcgatgctcg tccggaattt ccgaaagctc ctcgatgaac accgcgccga ggttttgcag
     1561 gccctcgacg acatagcggt tatgaacgat ctcgtggcgg acatagaccg ggcggccata
     1621 tttcttcagc gccagaacga cgatctggat ggcgcggtcg acgccggcgc agaagccgcg
     1681 tggctggcaa agcctgatgg tcaaaggcgg tttggtttga agcatgatgt ccgtaacagg
     1741 gctgtcggat cggtgtcggc cccgaaatgg gacgccgggc gatgccctgt caagggctgg
     1801 cgcctcattg cggctttgcg ggacgcctta gccagaagaa catgacgccg acaagtgccg
     1861 ccgccagccc ataccaggtg aatatatatt gcaagtggtt gttgggcagg tcgatgaggg
     1921 tgacaccgcc aatcggcagc ccgccgggat tggctgcgtt gttggcgtcg atgaaaatgg
     1981 gcgccagccg gtagccggca ggcagcccgg cggtggcggc catgacatca cggtccttcc
     2041 agtagaagat gttcttcacc ggatcgttgt cggggaccat gctggacggc ttgccgaaga
     2101 gcgggttacg ggcaagcccg gtgatggtga ccttgcctgc gacctggccc tggggccgct
     2161 tggcagcatc cttaagctcg taaggcacga agccacggtt gacgagcaga tagcggccat
     2221 cgtcgagatg cagcggcgta tagacatcga agcccgacgc gccgtcccag gtggcgaaga
     2281 aatgccgctc cgcttcatgc acgaaggtgc cggtcaccgt caccggcata tagtcgac
//
LOCUS       DASYCS010000175         1886 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4534653,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000175 DASYCS010000000
VERSION     DASYCS010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4534653"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..125
                     /locus_tag="VGM46_05650"
     CDS             <1..125
                     /locus_tag="VGM46_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006326999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HEY4192109.1"
                     /translation="RYELPMSEVSKKGGSAVLLQAVGKDGLPGPILGAAMIRKP"
     gene            complement(199..1209)
                     /gene="cydB"
                     /locus_tag="VGM46_05655"
     CDS             complement(199..1209)
                     /gene="cydB"
                     /locus_tag="VGM46_05655"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEY4192110.1"
                     /translation="MDHMLSFVPVWTLILAAGIFFYVLLDGFDLGVGILYGFEPDLQS
                     RNLVMNSIAPIWDGNETWLVLGGLALLAAFPLAFAIIVPALYFPVLVMLLALVFRGVA
                     FEFRFRDVEHKTFWDHGFAYGSAIATFAQGVMLGSYIQGFKIEGRHFVGTSLDFLTPF
                     SLLTGVALVFGYSLLGAGWLILKTEGDIQKRARKHGRISLIAVLAGVAIVSLWTPLVS
                     TEIAERWFSWPNIVILAPIPLVTLLTAFLVWRSLSGDSHAGPFIGAVILFALSYLGIA
                     ISLWPFIVPHTFTLWEAASSSSTQAFLLVGTLFLLPVILVYTGWSYWVFRGKVRADIG
                     YH"
     gene            complement(1209..>1886)
                     /locus_tag="VGM46_05660"
     CDS             complement(1209..>1886)
                     /locus_tag="VGM46_05660"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEY4192111.1"
                     /translation="GLNTLEHQPAKLAAMEALWETTTEMPLVLFTIPSDTAEANRFTV
                     EIPKLGSLILTHSLNGTVKGLKDFPPDQRPPVALPFFAFRAMVGIGILMLVLVASSWW
                     LRWRGRLFDSPNFLRACLFMGPMGFVAVIAGWTTTEVGRQPWVVYGLLRTADAVSPSL
                     TGWDVLTSLLFYMAVYLIIFPAGALIMFRIIRKGPQAGSADDTPTIEGGRPSQPITVE
                     LHSKGEA"
BASE COUNT          399 a          552 c          641 g          294 t
ORIGIN      
        1 agcgctatga attgccgatg agcgaggttt ccaaaaaggg cggcagcgcc gtcctgttgc
       61 aggcggtcgg caaggacggc ctgcctggac ctatccttgg cgcggcgatg atccgcaagc
      121 catagaggct tcgcctccgt ctacctgatt tcgtgtttga atgggccgaa ggcttttccg
      181 ctttcggccc cttatttttt agtggtagcc gatgtcggcg cgcaccttgc cgcggaacac
      241 ccaataggac cagcccgtat agaccaggat taccggcagc aggaacaggg tgccgaccag
      301 caggaaggcc tgcgtcgagc tggatgaagc ggcttcccac aaggtgaagg tgtgcgggac
      361 gatgaacggc cacaggctga tggcgattcc cagataggac agggcgaaca ggatgacggc
      421 gccgatgaac ggcccggcat gcgagtcgcc cgacagcgag cgccagacca ggaaagccgt
      481 cagcagcgtc accagcggga tcggcgccag gatcacgatg ttcggccacg agaaccagcg
      541 ttcggctatc tcggtgctga ccagcggcgt ccacaggctg acgatggcga cgccggccag
      601 caccgcgatc aggctgatgc ggccgtgctt gcgggcgcgt ttctggatgt cgccttcggt
      661 cttgaggatc agccagccgg cgcccagcag cgaatagccg aaaacgagcg ccacgcccgt
      721 cagcaatgaa aagggcgtca ggaaatcgag tgaagtgccg acaaaatggc ggccttcgat
      781 cttgaagccc tggatgtagg agccgagcat gacgccttgc gcgaaggtgg cgatggcgga
      841 gccgtaggcg aagccgtggt cccagaacgt cttgtgttcg acgtcgcgga agcggaactc
      901 gaaggcgacg ccccggaaca ccagcgccaa aagcatgacc agcaccggga aataaagcgc
      961 cggaacgatg atggcgaagg caagcgggaa ggcggccagc agggcgaggc cgccgagcac
     1021 cagccaggtc tcgttgccgt cccagatcgg ggcgatcgag ttcatcacca ggttgcgcga
     1081 ttgcaggtcc ggctcgaagc cgtagaggat gccgacgccg aggtcaaagc catccagcag
     1141 cacgtagaag aagatgccgg cggcgaggat cagcgtccag acggggacga agctcagcat
     1201 gtgatccatc atgcttctcc cttgctgtgc agttcgacgg tgatcggctg gctcggccgg
     1261 ccgccttcga tcgtcggcgt gtcgtcagcc gaaccggctt gcggaccctt gcggatgatg
     1321 cggaacatga tgagcgcgcc ggcggggaaa atgatgagat agaccgccat gtagaacaat
     1381 agcgaggtaa ggacatccca tccggtcagc gacggcgaaa ccgcgtcggc cgttcgcagc
     1441 aggccgtaga ccacccaggg ctggcgcccc acctcggtgg tggtccagcc ggcgatgacg
     1501 gcgacgaaac ccatcggccc catgaacagg caggcgcgaa ggaagttggg gctgtcgaac
     1561 agccgcccgc gccagcgcag ccaccaactg gaagcgacca ggacgagcat caatattccg
     1621 atgccgacca tggcgcggaa ggcaaagaag ggaagcgcca ccggcgggcg ttggtccggc
     1681 ggaaaatcct tcaggccctt caccgtgcca ttgaggctgt gggtgaggat caggctgccg
     1741 agcttcggta tctcgacggt gaagcggttg gcttcggcgg tgtcgctggg aatggtgaaa
     1801 agcaccagcg gcatttcggt ggtcgtttcc caaagcgcct ccatcgccgc cagcttggcc
     1861 ggctgatgtt ccagcgtgtt gagacc
//
LOCUS       DASYCS010000176         2520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_912709,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000176 DASYCS010000000
VERSION     DASYCS010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912709"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VGM46_05665"
     CDS             <1..302
                     /locus_tag="VGM46_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2778 domain-containing protein"
                     /protein_id="HEY4192112.1"
                     /translation="MRGPTPPNTYKLVMRESRFHGVEAIRLLPIDGRNKYGRDGLLAH
                     TYMLRGGRGQSNGCVVFEEYDRFLKAFKKGKISRLVVVPGRSRGGTRIAKNGQGA"
     gene            complement(345..659)
                     /locus_tag="VGM46_05670"
     CDS             complement(345..659)
                     /locus_tag="VGM46_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015317239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192113.1"
                     /translation="MATDQYHEPPEELSAETRTFARMVTSLIEEAEAINWYEQRISVE
                     KDAQARAIMQNAQKEEFKHFGMDLEFLLRKKSDWRVELKEILFTSGDIVAAGEKGEKK
                     AD"
     gene            complement(894..1172)
                     /locus_tag="VGM46_05675"
     CDS             complement(894..1172)
                     /locus_tag="VGM46_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006204886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192114.1"
                     /translation="MTDWLDILKQQTATGDQMSLAVTNMLADPAITQKQVKTLFSAIE
                     QQADFVEKLRMALEQFGHDFPIIRAAERLEERYGDLAANVAEKLKALA"
     gene            complement(1257..1703)
                     /locus_tag="VGM46_05680"
     CDS             complement(1257..1703)
                     /locus_tag="VGM46_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006204884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HEY4192115.1"
                     /translation="MLERKFTKIANKVAHITGLPLTFALCVAIVAVWAISGPIFQFSD
                     TWQLIINTGTTIVTFLMVFLIQNTQNRDGAAIQAKLDELIRVSTGHNHFIGIEHLTES
                     EVEEIRAKCERAATRHDEQIADMAAKKAVSRKNTAARKRSAKDKAA"
     gene            2002..>2520
                     /locus_tag="VGM46_05685"
     CDS             2002..>2520
                     /locus_tag="VGM46_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013530977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192116.1"
                     /translation="MTRKPLQRILPATAAASLCLLCGMASAHAEPGSSVSASSSLERH
                     WTSNALDSDHSVPDWYTLLRGSLQQQFGDDDANIGLGAEFQATRHDTVSIEDDRALAL
                     SAQAFRRFSPSLELRGTLTYRVSREGDDLPIGAFTLGTSTPKQILGASGQMGIDLGNA
                     TSLILDVADTYEK"
BASE COUNT          486 a          806 c          689 g          539 t
ORIGIN      
        1 aaatgcgcgg accgaccccg cccaacactt acaagctggt gatgcgcgaa tcccgctttc
       61 acggcgtgga ggctatccgc ctcctcccca tcgacggcag gaacaaatac gggcgcgacg
      121 gcctgcttgc ccacacctac atgcttcgcg gcgggcgcgg ccaatccaat ggctgcgtcg
      181 tcttcgagga gtatgaccgc ttcctgaagg cgttcaagaa gggcaagatt tcccgcctcg
      241 tcgtcgtgcc gggccgctcg cgtggcggca cccgcatcgc caaaaacggc cagggcgcct
      301 aaaattctag agcggctggc ttcggtaagc cgcttcaaaa ttgcctaatc ggcctttttc
      361 tcgccctttt caccggccgc cacgatgtcg ccgctggtaa acaagatttc ctttagttcg
      421 acccgccagt ccgacttctt gcgcagaaga aactccaaat ccatgccgaa atgcttgaat
      481 tcttccttct gggcgttctg catgatggcg cgcgcctgag cgtccttttc caccgatatg
      541 cgctgctcat accagttgat cgcttccgcc tcctcgatca gcgaggtgac catgcgggca
      601 aaggtccttg tctcggccga taattcttca ggcggttcat gatattgatc ggtagccatt
      661 ccgtattccc atgccggtta tcgcttgatc gctggatgag ccgtcgagat ttttcgacga
      721 cccctccacg cctgcaataa cgcgctcatg ctcgcgcggg ttccttccgt aggaacgccg
      781 gtcatctgac ggttgcagcc gctgttcggg gcagatgaaa gaaggctgac cagccggccc
      841 gcctgcatcg gcaggaccat gccggcgcat ccgtcaaaaa agccgtaaca agcctaggcg
      901 agcgccttca gtttctcggc gacgtttgcg gcaagatcgc cgtatcgctc ctccagccgc
      961 tcggccgcgc ggataatcgg gaaatcgtgc ccgaattgct caagcgccat ccgcagcttt
     1021 tcgacgaaat cggcctgctg ttcaatcgcc gaaaagagcg tcttgacctg cttttgtgta
     1081 atggccgggt cggccagcat attcgtcacc gcgaggctca tctggtcgcc ggtcgcggtc
     1141 tgctgcttca atatgtccag ccagtcggtc aatcgtcatc tccatgccgc cgcaccatgc
     1201 gcaagcaaat cggacagggt caacccacta gcaatcaagc ttcgggttca atccgatcac
     1261 gcggccttgt ctttcgccga acgtttccgc gctgcggtat tcttccgcga cacagctttc
     1321 ttcgccgcca tatccgcgat ctgctcgtca tggcgcgtgg cagcccgttc gcatttggcg
     1381 cggatttcct cgacctcgct ttcggtcaga tgctcgatgc cgatgaaatg gttgtggccg
     1441 gtgctgaccc ggatcaactc gtccagcttg gcctggatcg ccgcgccgtc gcggttctgc
     1501 gtgttctgga tcaggaacac catcaggaac gtcacgatcg tcgtgccggt attgatgatg
     1561 agctgccacg tatcggaaaa ttggaaaatc gggccgctga tcgcccacac ggccacgatg
     1621 gccacgcaaa gcgcgaaggt caaaggcagg ccggttatat gcgcgacctt gttggctatt
     1681 ttggtgaatt tccgttcgag catggtgcga tccttattgg ccaaggacaa aacattcggc
     1741 aggtgttcag gttcccctcg cccgattttg ccaacgcctt cgccagctcg ccaaaaaacg
     1801 acgcaaccac atacaactaa aagttatgta gatatagaat aacactttat acgattgatg
     1861 cgaaagtgct catgcaggca agagcctgtt ccttccgcct cacggcggtg tacccccggc
     1921 cccttccgtc tcgccacccc cgcaggcgga aggggcttgg tcccaaatgc cgaccacgcc
     1981 agcaagcttg cggtgttgaa gatgacccgg aaaccgctgc aacgaattct tcccgccacg
     2041 gcagcggcaa gcctgtgcct gttatgcggc atggcatccg ctcatgccga gccgggcagc
     2101 agtgtttccg cctcatcgag cctggagcgg cattggacga gcaacgcgct ggacagcgac
     2161 cattccgtcc ctgactggta cacgctgttg cgcggttcgc tgcaacagca gtttggcgac
     2221 gacgacgcca atatcggcct cggcgccgag ttccaggcga cgcgccatga cacggtttcc
     2281 atcgaggacg accgtgcact tgccttgtcg gcgcaggcct ttcgccgctt ttcgcccagc
     2341 ctcgaacttc gcggcacgtt gacctatcgc gtatcgcgcg agggcgacga cctgcccatc
     2401 ggcgcgttta cgctcggcac cagcacgcca aagcaaatcc tgggcgcatc gggccaaatg
     2461 gggatcgacc ttggcaacgc cacgtcgctc atcctcgatg ttgccgacac ttatgaaaag
//
LOCUS       DASYCS010000177        13998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4392007,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000177 DASYCS010000000
VERSION     DASYCS010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 13998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13998
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4392007"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="VGM46_05690"
     CDS             <1..691
                     /locus_tag="VGM46_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010914095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HEY4192117.1"
                     /translation="RRNMNPRMLVEFVDGSKTMVEMCAIANATGLVPDVPGMHGPRAD
                     RDDLAKVLVPREDGGILHKKGVVDYTVGKGVAPGVFVIVEATHPRIIERMNDLHIGHG
                     PYYSFFRPYHLTSLEVPLTAARIMLHGRPDMVPLPRPVAEVCAVAKRDLAAGETFDAI
                     GETCYRSWTMTVGDARAQHAIPVGLLEGGKVLKPVRKGELLTAANAAPDPTTRLFALR
                     KRQDEMLYGAG"
     gene            complement(696..1511)
                     /gene="proC"
                     /locus_tag="VGM46_05695"
     CDS             complement(696..1511)
                     /gene="proC"
                     /locus_tag="VGM46_05695"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEY4192118.1"
                     /translation="MTVRLVLVGSGNMGYAMLAGWINSGKLGAAETLVVEPNADLRQR
                     AAKLGCQTAADAGEIAADAAPQMVVIAVKPQVIRAATEAYARFGDGRTTFVSVAAGTP
                     VATLEEVLGKRAPIMRCMPNTPAAIGKGMMVLFSNPLVSDETKRTVADLLSASGKVAT
                     ISDESLMDAVTAVSGSGPAYIFHFIEALTVAAEKAGLPTETARLLAMQTVYGAASLAA
                     ESSDEPGELRRQVTSLNGTTAAALAVLMGEDRLTHLLTDAVEAARLRSVELGK"
     gene            complement(1546..2889)
                     /locus_tag="VGM46_05700"
     CDS             complement(1546..2889)
                     /locus_tag="VGM46_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase"
                     /protein_id="HEY4192119.1"
                     /translation="MSNIGSLKRTERAPLSAAFTREGVATATAALLLTVILVSFRPFQ
                     PEGSGVSGSDIVNQIGFGALGAISIFSLAAFAEPRVVRSLLSPSWFVMLAVFMLSVAM
                     ATDPLSALRTASFTLIGMMAMATVLALPRDADAFSTVLIFTAAAVIGLSYVGLVVFPN
                     EAMHTVNSSEPEQAGLWRGVFTHKNIAGPVMACFSFAGLYLYRRGRRLAGATIFCAAI
                     FFMAHTGSKTTLALVPFSILIVMLPGLLGMRLGTPVLFAMAIVATAIGTLGIVFLPPL
                     SHFVAANFPDLTYTGRTTIWEFAGSMLAQRPWTGYGYESFWGTPLLLEQDQPFDRAWD
                     VRNIVHGHNGYLDIAVLMGIPAACIAAYTFVCAPMRDYMRIPLRQENVWLGDFFMMVV
                     FFTALNAFLESFFFRRADPVWMFFVLAAFGLRQVSLRPIAGDNSGQALPRRAANG"
     gene            complement(2858..3859)
                     /locus_tag="VGM46_05705"
     CDS             complement(2858..3859)
                     /locus_tag="VGM46_05705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013530225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY4192120.1"
                     /translation="MNAHLPSDGSISVEGRSPGLDAEAIEVVVTLPTFKRPAQVLATL
                     DSLMRQETRRRFAIVLIENEAEAREGARAAKPLFDNGTFPGLVIVAHRRGNCSAYNAG
                     WQTALAYFPNFKHLLVIDDDELAEPQWLERMCRTAETLPADIVGGPQVPAFSEPGHER
                     WADHPVFAPPYRKTMRVPALYSSGNLLVGRPVLEAMGPQFLDLKFNFMGGGDSDFLSR
                     ASQKGFVLGWCAEARTLEAVPARRVEADWIRARSLRNGVISTLVEKKKREGMPFARTR
                     VVLKSLALLAVSPFRGLLRLARTGSTMTALYPVHVALGRVFAEFGYANEQYRQPEKN"
     gene            4030..5220
                     /locus_tag="VGM46_05710"
     CDS             4030..5220
                     /locus_tag="VGM46_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008877472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY4192121.1"
                     /translation="MHLLFATSIVPDGALASGYEIANAAIIEALRRAGARVTVMGFSW
                     PGKPAIDPQNTIVLGSLDVRTDSASPMQKLAWTAKAMATGLTFASAKLRVVTEAEIRA
                     AIRHAGPFDGYVLNSVQFAGAFERLFGDLPSIFVAHNVEHKSAQENAAAARSLPERLL
                     FKREARLLEAMEARLCRRARFVFTLAEEDRAALGVASDDRSAVLPLTTRPSAPPKQAR
                     RIECDTALIGTWTWQPNRIGLDWFLGRVVPHLRPDFSIRIAGSMPAGVKSPHPGVSFV
                     GRVPDAQAFVRSAAVIPLISTAGSGVQLKTIETFELGLPSVATTRSLRGIGYRAENCV
                     VTDDPVAFARALEDAVAVKVADVDGAAFHRHQLRAMDAAIARGLQAIGQVGSAGQVAQ
                     EAFA"
     gene            5217..6011
                     /locus_tag="VGM46_05715"
     CDS             5217..6011
                     /locus_tag="VGM46_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006329311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEY4192122.1"
                     /translation="MNMHTARKALGAYESKTVLGIPVVSLGWNDAVALLSRLLVERRF
                     TKVTFLNAHNANIAYTDPGFAKILDDFLILPDGVGVDMAAKLLYGAPFPANLNGTDFV
                     PAFLEATTRPLTVALLGASPEIAEAASARLGEMAPQHRLVVIHNGYFSPGEEAGILNR
                     IAELHPDILLVAMGVPRQEMWIERHIDARHCTLPIAVGALLDFLSGVVPRAPLWIRRL
                     RLEWVFRLAIEPRRLWRRYVVGNPLFLWRVLRQKLFGKAEIVEAKR"
     gene            6071..6931
                     /locus_tag="VGM46_05720"
     CDS             6071..6931
                     /locus_tag="VGM46_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6492 family protein"
                     /protein_id="HEY4192123.1"
                     /translation="MAIVTPSYVHDFERCRLLCETLDRHVSGAAHHYILVEHRDMALF
                     VQLRGPHRTIVDERELLPGWLRPFNDPSSLFRRRIWLSWKTKPLRGWHVQQLRRIAIA
                     AHVPQDVLVFCDSDVVFVRPFDCGVFQRDGKVRLFRRDHTMEGEVRYDHRLWSQNAGV
                     ALGIASPAVSPHDYISTLIAWRRETIGAMCRRIEEQHGRSWIEVLGSSRKFSECMVYG
                     RYVDEVTEGADHFHGSEEFCHVHWFGEALSDEGFLDFLAAMAPEQAALGVQSFLGVDP
                     DRIRRLLGLR"
     gene            complement(6928..8223)
                     /locus_tag="VGM46_05725"
     CDS             complement(6928..8223)
                     /locus_tag="VGM46_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010914102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEY4192124.1"
                     /translation="MAQATNIPDKRSLGRLGALLTDRRGVVYDYLSAVSGAGGRLVFS
                     LVYFIALANTLSIAEFGLFATASAAGVMLSRVLAFGFISALYRTSAKRPNLIGAFTAG
                     FIVLGLLSLPVLAAASYGVYLLFFAANMALPVFATVVCAEALLWRPVEVAMIVNNGLG
                     KFGRAALLSILTSVLRALGAVLFMMAARHSLGIWAWYYIGANAASLLLAFGVFYPRQR
                     LRFRAELYWRRLADSVYVACAEVLFYLQMEFDKLLVLAIGGPHLAGIYAIIMRLVDLT
                     AIPIRTFSMMLVQKMMRQPAILSRLAVRCGIEGGVFAVSTLGLLALGTILHFFPTALG
                     RNVAEAAPLVLLAICIPGLRNLVEYQAELLFARGQTLVRALNLALLAGAKAVLLTYVL
                     TRITGTPDLVLSLNIVFLLLYLVSALLTYSAMRQPAKAV"
     gene            8376..10856
                     /locus_tag="VGM46_05730"
     CDS             8376..10856
                     /locus_tag="VGM46_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013530230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HEY4192125.1"
                     /translation="MTHAVRAPPKVKPQAMFDRENREDKSRERPFSAPDRRVDEPAGP
                     AAPFPASVDENEPGDTAFATRAVETAPDRAYEDGERWRPLIDPMKVVGGVARSKKIIA
                     ATTLAGALLGVALALSTPKKFEAATELIADPRDLQITDHDLTQSGLASDTTLAIVENQ
                     VRVLTSGTVLNQVAEKLNLFDDPEFNGQAKGFPGVMTLIRSMLSRDDGAVAGGDDRLR
                     TVVIRNLAESLSVERGGKTFVITIGAKTENGEKSALIANTMADVFLQTYGKMQSDTAG
                     RATDELTGRLGELRKAVETAERKVETFKSEHDLVDAQGRLISDDELLKLNEQLTVARA
                     RTLELNAKAASARSVDVDSVVAGTLPEEISSNAMNDLRSQYAKLKQEADRADVRLGPR
                     HPERLALDAQLAGARERIAAELKRIGSSLQTDLKRAVQLEQQLSSRLAQLKVRSGDVN
                     GDQVTARELERDANAKRAVYEAFLLRAKQTGEQKDINTANISVISKAYAPLDPTGPSR
                     ALTVLAGLLLGFASGVGLGAMRGVYASLRDTADGRSRRAVPAPRRVAAVEMTPPPRPA
                     APPVPAEPELAEATAPIEPRPNPVESLRAAIGGALSRRRSNFGETVEPTPAETYPETY
                     EETQAGFDDDMPFAAEDAMQQTESSMYPYYADPNAPFAPQQPAMGYPQPQFQQAPQFQ
                     PGMYPQQPYSAQASYYPGGQPPHHPAPQMQPQSFAPAQSYAPQPYAPQPYAAQPYPHM
                     QQPAGYPPAQVWPMQQAPQAYPYGQPAPHHAGPMHYAPSPYAYPQMQEAQGAADEQTP
                     IEEIRASLREFRDAVRDLAESRSRRRYF"
     gene            11061..11960
                     /locus_tag="VGM46_05735"
     CDS             11061..11960
                     /locus_tag="VGM46_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEY4192126.1"
                     /translation="MAELIDGKSIAADVVDMVKAMTADLFAKTGMKPGIAVIIVGEDP
                     ASQVYVASKSRTAKECGFLSVQYTLPADTAEAPLLKLIDELNADPAIHGILVQLPLPG
                     HVDAGKVTQAIAPEKDVDGFHFINVGKLGAGELETAFVPCTPAGSMLLIERARGNDLS
                     GLNAVVLGRSNIVGKPMANLLLAANCTVTIAHSRTKDLPALARTADILVAAVGRPQMV
                     KGDWIKPGATVIDVGINRIPAPEKGEGKMRLVGDVAFAEAEKVAGAITPVPGGVGPMT
                     IAMLMANTLVSAYLAAGLKRPSF"
     gene            11978..12991
                     /locus_tag="VGM46_05740"
     CDS             11978..12991
                     /locus_tag="VGM46_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HEY4192127.1"
                     /translation="MKAPALPTISAPPERPALGDAVQGGRQFAFLLVDKFSMFSLAAA
                     IDTFRSANRLLGRDFYGWTTVSADGDAVIASNGLPIKIDYAVADIPPVDIIFVAVGLT
                     TEFPGKSKVLGALRSWGRRGGALGALSVGSYLLAEAGQLEGCRCTIHWENRAGFLERF
                     PDINCTGNVFEIDRKRYTCAGGTTSIDLMLEIVRGDLGPNVANGVANQFQHERIRSAG
                     DRQRVGPERDLTGKSEKLRRIVELMADHLDEPLSAVQLAKSAGLSVRQVERLFLRHLT
                     VTPGRYYMRLRLERARELLRQTNMPILDVAIATGFTSHSYFAQSYRLQFGRPPSEERR
                     TTY"
     gene            13072..>13998
                     /locus_tag="VGM46_05745"
     CDS             13072..>13998
                     /locus_tag="VGM46_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006329329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY4192128.1"
                     /translation="MTALLRNLLGVLFLLTSATASLASGQVIVILDASGSMWAQIDGR
                     PKQEIARESLRKALASVPADAEIGFMAYGHSDKASCDDIDLIVSPAAGTASAISSAAD
                     NLKFLGKTPLTAAVKKAAETLNHTQGASKIVLITDGTESCEGDPCALGRELKAAGSGL
                     IVDVVGFGLSAQDGKQVACLAQSTGGKYIEASDAKALQAALVESVAATPAARATSAAS
                     AQATPETATPPTAEAAKPEFNFLPTVTLAQGGAPITQGNAWEIYKAAANGTRGASIAT
                     EYGAYQGNLEPGDYILVARDGEAKAEQPLKVEA"
BASE COUNT         2594 a         4625 c         4426 g         2353 t
ORIGIN      
        1 ccgccgcaac atgaacccgc gcatgctggt cgagttcgtc gacggctcca agacgatggt
       61 ggaaatgtgc gccatcgcca acgccaccgg gctggtgccg gatgtgccgg gcatgcacgg
      121 ccccagggcc gaccgcgacg atctcgccaa ggtgctggtg ccgcgcgaag atggcggcat
      181 cctgcacaag aaaggcgtgg tcgactacac ggtcggcaag ggcgtcgcgc ccggcgtttt
      241 cgtcatcgtc gaagccacgc atccgcgcat catcgagcgc atgaacgacc tgcatatcgg
      301 ccacggcccc tattatagct tcttccggcc ctaccacctt acctcgctgg aagtgccgct
      361 gacggcggcg cgcatcatgc tgcacggcag gccggacatg gtgccgctgc cgcgcccggt
      421 cgcggaagtc tgcgcggtgg ccaagcgcga cctggcagct ggcgaaacct tcgacgccat
      481 cggcgagacc tgctaccgct catggacgat gacggtcggc gacgcccgcg cgcagcacgc
      541 aatccccgtc ggcctgcttg aaggcggtaa ggtgctgaag ccggtcagga agggcgaact
      601 gctgacggcg gccaacgccg cgcccgaccc gacgacgcgc cttttcgcgc tgcgcaagcg
      661 ccaggacgaa atgctttacg gcgcgggctg agtatttatt tcccgagttc gaccgaacgc
      721 agccgggctg cttccaccgc gtccgtcagc agatgcgtca gccgatcctc acccatcagc
      781 acagcaaggg ccgccgccgt ggtgccgttc aggctggtca cctgccggcg caattcgccc
      841 ggctcgtcgc tgctttcggc ggcaagcgaa gccgcgccat agacggtctg catggccagc
      901 agcctggccg tttccgtggg aaggccggcc ttttcggcgg ccaccgtcaa tgcctcgatg
      961 aaatggaaga tataggccgg ccccgaaccg gaaacggcgg tcaccgcgtc catcaggctt
     1021 tcgtcggaaa ttgtcgccac cttgccgctc gccgaaagca ggtcggcaac ggtgcgcttc
     1081 gtttcatcgg aaaccagcgg gttggaaaac agcaccatca tgcccttgcc gatggcggcg
     1141 ggcgtattcg gcatgcagcg catgataggc gcgcgtttgc ccagcacctc ctccagcgtg
     1201 gcgaccggcg tgccggcggc gacgctgacg aaggtggtgc ggccgtcgcc aaagcgggca
     1261 taggcctcag tagcggcgcg aatgacctgc ggcttcaccg cgatcaccac catctgcggt
     1321 gcggcgtcgg cagcaatctc gccggcatcg gcggcggttt ggcagccgag ctttgccgcc
     1381 cgctggcgca gatcggcgtt cggctcgacg acgagcgtct cggcggcgcc cagcttgccg
     1441 gaatttatcc agccggccag catggcgtaa cccatattgc cgctgccgac gagaacaagc
     1501 ctgaccgtca tcgaaatcct ccggaaataa ttgccgcgaa ccgctctaac cgttcgccgc
     1561 gcgccgggga agggcctggc cggaattgtc gccggcaatc gggcgaagcg aaacctgccg
     1621 caggccgaac gccgccagga cgaagaacat ccatacgggg tcggcgcgcc ggaagaaaaa
     1681 gctttccagg aaggcgttga gagcggtgaa gaacaccacc atcatgaaga aatcgccgag
     1741 ccagacgttt tcctggcgca gcggaatgcg catgtaatcg cgcatcggcg cgcagacgaa
     1801 ggtgtaggcg gcaatgcatg cggcggggat gcccatcagc acggcgatgt cgagatagcc
     1861 attgtggccg tggacgatgt tgcgcacgtc ccatgcgcgg tcgaagggct ggtcctgctc
     1921 cagaagcagc ggcgtgcccc agaagctttc atagccgtaa cccgtccatg gccgttgagc
     1981 cagcatgctg ccggcaaact cccagatcgt cgtgcgcccg gtataggtca ggtccgggaa
     2041 gttggcggcc acgaaatggc tgagcggcgg caggaagacg atgcccagcg tgccaatggc
     2101 cgtggcgacg atcgccatgg cgaacagcac cggcgtgccg aggcgcatgc caagcaggcc
     2161 gggcagcatg acgatcagga tcgagaaagg caccagcgca agcgtggtct tggagccggt
     2221 atgggccatg aagaagatcg ccgcgcaaaa gatggtcgct cccgccagcc gccgtccgcg
     2281 tcggtagaga taaaggccgg caaagctgaa gcaggccatc accgggccgg cgatgttctt
     2341 gtgggtgaac acgccgcgcc acaggccggc ttgttccggt tcactggaat tcaccgtgtg
     2401 catcgcctcg ttgggaaaca ccaccagccc cacataggac aggccgatga cggcggcggc
     2461 ggtgaagata agcacggtcg aaaaggcgtc ggcatcgcgc ggcagcgcca gcaccgtcgc
     2521 catcgccatc atgccgatca gcgtgaagga tgccgtgcgc agcgccgaca gcggatcggt
     2581 cgccatcgcc accgaaagca tgaagacggc cagcatcaca aaccaggatg ggctgagcag
     2641 cgagcgcacg acgcgcggtt cggcgaaggc cgcaagcgag aagatcgaaa tcgcccccag
     2701 tgcgccgaag ccgatctggt tgacgatatc gctgccgctg acgcccgatc cttccggctg
     2761 gaatggcctg aacgacacca gaatgacggt caacagcaac gccgcggtcg cggtggcgac
     2821 gccctcgcgg gtgaaggccg cgctcagcgg cgcgcgttca gttcttttca ggctgccgat
     2881 attgctcatt ggcgtatccg aattcggcaa aaacgcgccc gagcgcgaca tggacggggt
     2941 aaagcgcggt catggtcgag ccggttctcg ccagccgcag cagcccgcga aacggcgaga
     3001 cggcaagcag cgccaagctt ttcagcacca ccctggtgcg ggcaaagggc atgccttccc
     3061 gctttttctt ctcgaccagc gtcgagatga cgccgttgcg caggcttctg gcgcggatcc
     3121 aatctgcttc gacgcggcgc gccggcacgg cctccagcgt gcgcgcctcc gcgcaccagc
     3181 caagcacgaa gcccttttgg gaagcgcggc tgaggaaatc cgaatcgcca ccgcccatga
     3241 agttgaattt gaggtccagg aactgcggtc ccatcgcttc gagcacgggc cggccgacca
     3301 gcaggttgcc cgacgaatag agcgccggca cgcgcatcgt cttgcggtag ggcggtgcga
     3361 ataccggatg gtcggcccag cgctcgtggc cgggttcgga gaacgccggc acttgcggcc
     3421 cgccgacaat gtcggccggc agcgtctcgg cggtccggca catccgctcc agccattgcg
     3481 gttcggccaa ttcgtcgtcg tcgatgacca gcaggtgctt gaaattggga aaatacgcca
     3541 gcgccgtctg ccaaccggca ttataggcgc tgcaatttcc gcgccggtgc gcgacgatca
     3601 ccagtccggg aaacgtgccg ttgtcgaata atggcttcgc ggcgcgggcg ccctcgcgcg
     3661 cctcggcctc gttttcgatc aagacgatgg cgaagcgtcg gcgggtttcc tggcgcatca
     3721 gcgaatccag cgtcgccagc acctgcgccg gccttttgaa ggtcggcagc gtcaccacca
     3781 cctcgatggc ctccgcatcc agtcccggcg accggccttc gaccgatatc gagccatccg
     3841 atggcaaatg agcattcatc tttttcaggc cgcttgttca ggaccgttgc atagcacctt
     3901 ggcgataacg gttcgttaat caccttgcgc ggttgcgata gaaaatgctt ggcccggagt
     3961 tggcaaatgc cggcttttaa gggccggttc accccgctct gctaatggat cggctacagg
     4021 ataggcccga tgcatctgct gtttgccaca tcgatcgttc ccgacggcgc tctcgcctcc
     4081 ggctatgaga ttgccaacgc ggcgatcatc gaagcgctgc gccgtgcagg cgcgcgcgtc
     4141 acggtcatgg gtttctcctg gccgggaaag cctgccatcg atccgcaaaa caccattgtg
     4201 cttggctcgc tcgacgtgcg caccgacagc gcatccccga tgcaaaagct tgcctggaca
     4261 gccaaagcga tggccaccgg cctgaccttc gcctcggcaa aactgcgcgt ggtcaccgaa
     4321 gccgaaattc gcgcggccat ccggcatgcg gggccatttg atggctatgt cctgaattcg
     4381 gtgcagttcg ccggcgcgtt cgagcgcttg tttggcgatc tgccgtcgat tttcgtcgcc
     4441 cataatgtcg agcataagtc ggcgcaggaa aacgccgccg ccgcgcgcag cctgcccgaa
     4501 cgcctgttgt tcaagcgcga ggcaaggctg ctggaggcga tggaagcgcg gctttgccgg
     4561 cgcgcccgct ttgtcttcac gctggcggaa gaagaccgcg cagcacttgg cgtcgcctcg
     4621 gacgaccgtt ccgccgtgct gccgctgacc acgcgcccca gcgcgccgcc gaagcaggcg
     4681 cgccggatcg aatgcgatac agctctgatc ggcacctgga catggcagcc gaaccgcatc
     4741 ggcctcgact ggttcctcgg gcgtgtcgtg ccgcacttgc ggccggattt cagcatccgc
     4801 atcgccggca gcatgccggc cggggtgaag tcgccgcatc ccggcgtcag cttcgtcggg
     4861 cgcgtgccgg atgcgcaggc gttcgtgcgc agcgccgccg tcatcccgct gataagcacc
     4921 gccggcagcg gcgttcagtt gaaaaccatc gagacattcg aactcggcct gccatcggtc
     4981 gccaccacac gctcgctgcg cggcatcggc tatcgtgccg aaaattgcgt ggtcaccgac
     5041 gatcccgtcg cctttgcccg cgcgctggaa gatgcggttg ccgtcaaggt ggccgatgtc
     5101 gatggcgcgg cctttcaccg ccatcagttg cgggcgatgg atgcggcgat tgcgcggggc
     5161 ttgcaggcga tcggccaggt cggctcggcc ggccaagtcg cacaggaggc gtttgcgtga
     5221 acatgcatac ggcccgaaag gcgcttggcg cctacgaaag caagacggtg ctcggcattc
     5281 ccgtcgtcag cctgggctgg aacgatgccg tggcgttgct gtcgcgcctg cttgtcgagc
     5341 ggcgcttcac caaggtgacc ttcctcaacg cccacaatgc caacatcgcc tatacagatc
     5401 ccggctttgc gaaaatactg gatgatttcc tcatccttcc agacggggtc ggcgtcgaca
     5461 tggcggccaa actgctttac ggcgcgccgt tcccggccaa tctgaacggc accgacttcg
     5521 ttccggcctt cctggaggcg acgacgcggc cgcttaccgt ggcgctgctt ggcgcatcgc
     5581 ctgagattgc cgaggcggcg tcggccaggc tcggcgaaat ggccccgcag caccgtctgg
     5641 tggtcattca caacggttat ttttcacccg gcgaggaagc gggcatcctc aaccgcatcg
     5701 ctgaactcca ccccgatatc ctgcttgtcg ccatgggcgt gccgcgccag gaaatgtgga
     5761 tcgaacggca tatcgacgcc cgccattgca cgctgccgat cgccgtcggc gcgctgctcg
     5821 atttcctgag cggcgtggtg ccgcgcgcgc cgttgtggat acgccggttg cggctggaat
     5881 gggtgttccg gctggccatc gagccgcgcc ggctatggcg ccgctacgtt gtcggcaatc
     5941 cgctgttcct gtggcgcgtg ctgcgtcaga agctgtttgg aaaagccgag atcgtcgagg
     6001 caaagcggtg aacagccggt tcgcgccgca agaagcggcc aggcccgaaa tagccgcgcc
     6061 acccgccgcc atggccatcg tcacgccaag ctatgtccat gatttcgaac ggtgccggct
     6121 tttgtgcgaa acgctcgatc gccatgtcag cggtgcggcg caccactaca tcctggtcga
     6181 gcaccgcgac atggcgctgt ttgtgcaatt gcgcgggccg catcgcacca ttgtagacga
     6241 gcgcgagctt ctgcccggct ggctgcgccc gttcaacgac ccctccagcc tgtttcgccg
     6301 gcgcatctgg ctcagctgga aaaccaagcc gctgcgcggc tggcatgtgc agcaactgcg
     6361 ccgcatcgcc atcgccgcgc atgtgccgca ggacgtgctg gtgttttgcg attcggatgt
     6421 cgtgttcgtc aggccgttcg attgcggcgt ctttcagcgc gatggcaagg ttcgcctgtt
     6481 tcgccgcgac catacgatgg aaggcgaggt gcgctacgac caccgcctgt ggtcgcaaaa
     6541 tgctggcgtg gcgcttggca tcgccagccc ggcggtatcg ccgcacgact atatctcgac
     6601 gctgatcgcc tggcggcgcg agacgatcgg cgccatgtgc cggcgcatcg aggagcagca
     6661 cgggcgcagc tggatcgaag ttctcggttc aagccggaaa ttctccgaat gcatggtcta
     6721 tggtcgctat gtcgatgaag tcaccgaagg cgcggatcat ttccatggct cggaagagtt
     6781 ctgccacgtc cactggttcg gcgaggcgct ttccgacgaa ggtttcctgg acttcctcgc
     6841 cgccatggct ccggagcagg ctgcacttgg cgtgcagtct ttcctcggcg tcgatcccga
     6901 ccgcattcgc cggctgctgg ggctgcgcta aaccgctttt gccggctggc gcatcgccga
     6961 ataggtcagc agcgccgaga caagatagag cagcaggaac acgatattga gcgacagcac
     7021 gagatccggc gtcccggtga tccgcgtcag cacataagtc agcaataccg ccttcgcgcc
     7081 ggcaagcagc gcgaggttca gcgcgcgcac cagcgtctgg ccgcgcgcga acagaagctc
     7141 cgcctggtac tcgaccaggt tgcgcaggcc tggaatgcag atcgccagca ggaccagcgg
     7201 cgcagcctcc gccacgttgc ggccaagcgc cgtcgggaag aaatgcagga tggtgccgag
     7261 cgccaaaagg ccgagcgtcg agacggcgaa cacgccgcct tcgatgccgc atcgaacggc
     7321 cagccgcgaa aggatcgccg gctggcgcat catcttctgc accagcatca tcgagaaagt
     7381 acggatggga atggcggtga gatcgaccag ccgcatgatg atggcgtaga tgccggccag
     7441 atgcggcccg cctatcgcca gcaccagcag cttgtcgaac tccatttgca ggtaaaacag
     7501 cacctccgcg caggcgacat agacggaatc ggcaagcctg cgccaataaa gctcggcgcg
     7561 aaaacgcagc cgttggcgcg ggtagaacac gccgaaggca agcagcagcg aggcggcgtt
     7621 ggcgccgata tagtaccagg cccagatacc cagcgagtgc ctggcggcca tcatgaacag
     7681 caccgcgccc agcgcccgca gcaccgaggt caggatcgac agcagtgctg cgcgcccgaa
     7741 tttgccaagg ccgttattga cgatcatcgc cacctcgacc gggcgccaca gcagcgcttc
     7801 cgcacacacc accgtcgcaa acaccggcag cgccatgttg gcggcgaaga acagcagata
     7861 gacgccgtag gatgccgccg ccagcaccgg cagcgacagc aagccaagca cgatgaagcc
     7921 ggccgtgaac gcgccgatca ggttggggcg cttcgccgag gtgcggtaaa gcgccgaaat
     7981 gaagccgaag gcgagcaccc gcgacagcat gacgccggcc gccgaagccg tggcgaacag
     8041 gccgaattcg gcaatcgaaa gcgtattggc gagcgcgatg aaatagacca gcgaaaacac
     8101 cagccgtccg cccgcgccgc ttaccgccga caaatagtcg tagacgacgc cgcgccggtc
     8161 ggtcagcagg gcgccgagcc tgccgaggct gcgcttgtcg gggatgtttg tcgcctgtgc
     8221 catggttggc ggtgatgcta cacggcaaag tttaatgcgc ggtgttgcca tcgcccgctt
     8281 cgcgccctga tcaagtggcc gtcggaagaa aacgccgttg cggcggctca aaattaacct
     8341 tttgtttcct atgcctgttt agcgtggctt aacgaatgac gcatgctgtt cgcgcgccgc
     8401 cgaaagtaaa gcctcaagcc atgttcgaca gggaaaaccg cgaagacaaa agccgtgagc
     8461 gcccgttttc cgcgccagac cggcgcgtgg acgaaccggc tgggccagct gcgccgtttc
     8521 ccgcttccgt cgacgaaaac gagccgggtg acaccgcctt cgccacccgc gccgtcgaaa
     8581 cggcgccgga tcgagcctat gaggatggtg agcgctggag gccgttgatc gatccgatga
     8641 aggtggtggg tggcgtcgcc cgttcgaaga agatcatcgc cgccaccacc ctggcgggcg
     8701 cgttgctggg cgtggcgctg gcgctttcga cgcccaagaa attcgaggcg gcgaccgagc
     8761 tgatcgccga tccgcgcgac ctccagatca ccgatcatga tctcacccag tccggcctcg
     8821 cttcggacac gacgctggcc atcgtcgaaa accaggtgcg ggtgcttact tccggcacgg
     8881 tgctgaacca ggtcgccgaa aagctcaacc tgttcgacga tccggaattc aacgggcagg
     8941 caaaaggctt tcccggcgtg atgacgctga tccgctccat gctttcgcgc gatgacgggg
     9001 cggtcgccgg cggtgacgac cgcctccgca cggtcgtcat ccgcaatctg gcggaaagcc
     9061 tgtcggtcga gcgcggcggc aagaccttcg tcattaccat cggcgcgaag accgagaacg
     9121 gcgaaaagtc ggccctgatc gccaacacca tggccgacgt attcctgcaa acctacggca
     9181 agatgcagtc ggacaccgcc ggccgcgcca ccgacgaact gacgggccgt ctgggcgaac
     9241 ttcgcaaggc ggtggaaacc gccgagcgca aggtggaaac cttcaagtcc gagcacgatc
     9301 tggtcgacgc gcagggccgc ctcatctccg acgacgagct gctcaagctg aacgaacagc
     9361 tcacggtcgc gcgcgcccgc acgctggaac tcaatgccaa ggcggcctcg gcccgctccg
     9421 tcgatgtcga ttcggtggtt gcaggcacct tgccggagga aatcagctcg aacgcgatga
     9481 acgacctgcg ttcgcaatat gccaagctca agcaggaggc ggaccgcgcc gatgtgcggc
     9541 ttgggccacg ccatcccgag cggctggcgc tcgacgccca gcttgccggc gcgcgcgagc
     9601 gcatagcggc cgaactcaag cgcatcggct cttcgcttca gacggatttg aagcgcgcgg
     9661 tgcagctgga gcagcaattg tcctcgcgcc tcgcgcagtt gaaggtgcgc agcggcgacg
     9721 tcaatggcga ccaggttacc gcgcgcgaac tggaacgcga tgccaacgcc aagcgcgcgg
     9781 tgtatgaggc gttcctgctg cgcgccaagc agacgggcga gcagaaagac atcaataccg
     9841 ccaatatcag cgtgatttcc aaggcttatg cgccgcttga cccgaccggg ccgtcgcgcg
     9901 ccttgaccgt ccttgccggc ttgctgctcg gttttgcatc cggcgtcgga ctgggcgcga
     9961 tgcgcggggt ttatgcaagc ttgcgcgata ccgccgatgg ccgctcgcgc cgcgccgttc
    10021 cagccccgcg ccgcgtggcg gccgttgaaa tgacgccgcc gccacggccc gccgcaccgc
    10081 ctgttccagc cgaacccgag cttgccgagg ccacggcccc catcgagccg cggcccaacc
    10141 cggtcgagag cttgcgggca gcgatcggcg gcgcattgtc gcgccgaagg tcgaattttg
    10201 gcgaaacggt cgagccgacg cctgccgaga cttatccgga aacttacgaa gaaacccagg
    10261 ccgggttcga cgacgatatg ccgttcgccg ccgaagacgc catgcagcaa acggagagct
    10321 ccatgtatcc ctactacgcc gatccgaacg ccccgtttgc tccgcagcag ccggcgatgg
    10381 gctatccgca gccgcaattc cagcaggctc cccagttcca gcccggcatg tatccgcagc
    10441 agccatattc ggcgcaggcg tcttattatc cgggcggaca gccgccgcat catcccgcgc
    10501 cccagatgca gccacagtct ttcgcgcccg cccaatctta cgcgccccaa ccctacgcgc
    10561 ctcagcctta cgccgcccag ccttatcccc atatgcagca gcccgccggc tatccgccgg
    10621 cgcaggtctg gccaatgcag caagcgccgc aggcttaccc ctacgggcaa ccggcgccac
    10681 accatgcggg gccgatgcat tacgcgcctt cgccctatgc ctatccgcag atgcaggagg
    10741 cgcaaggcgc agccgacgag cagacgccga tcgaagaaat tcgcgccagc ctgcgcgagt
    10801 tccgcgatgc ggtgcgcgac ctggccgaaa gccgctcgcg ccgccgctat ttctgaaacc
    10861 tcggataaaa gcgccggctt tcgggtttac ggcatcatgc ggcgcattgt cgggcttgcg
    10921 tgatctgatt ttcatgaaat gctagatatg aggcccatcc tccggtcacg cgggataggc
    10981 ctcgaatttc agtttgtgcg cggtccttca gtgggaatct cctgccgtct gccgcgcgcc
    11041 ggtagcagga aagatggcgc atggccgagt tgatcgatgg aaagagcatc gccgccgacg
    11101 tggtggacat ggtgaaggcg atgacggccg atcttttcgc caaaaccggc atgaagcccg
    11161 gcatcgcggt gatcatcgtc ggcgaggacc cggccagcca ggtctacgtc gcctccaagt
    11221 cgcgcactgc caaggaatgc ggttttcttt ccgtccagta caccttgccc gccgacacgg
    11281 ccgaggcgcc attgctgaag ctcatcgacg aattgaacgc cgatcccgcc attcacggta
    11341 ttctggtgca gttgcccttg cccggccatg tcgatgccgg caaggtcacc caggcgatcg
    11401 cgccggaaaa ggatgtcgac ggctttcact tcatcaatgt cggcaagctt ggcgccggcg
    11461 agcttgaaac ggctttcgtg ccctgcacgc cggccggctc catgcttttg atcgagcgtg
    11521 cgcgcggtaa tgatctctcc ggcctcaacg cggtagtgct cggccgctcc aacatcgtcg
    11581 gcaagccgat ggccaacctg ttgctggccg ccaattgcac cgtcaccatc gcccacagcc
    11641 gcacgaagga tttgccggcg ctggcgcgta ccgccgatat tctggtcgcc gccgtcggtc
    11701 gtccccaaat ggtcaaaggc gactggataa agccgggtgc gaccgtcatc gatgtcggca
    11761 tcaaccgcat tcccgcgcct gaaaagggcg agggcaagat gcgcctggtc ggcgacgtcg
    11821 cctttgccga ggccgaaaag gtcgccggcg ccatcacgcc cgtaccgggc ggtgtcggcc
    11881 cgatgaccat cgcgatgctg atggccaaca cgctggtttc cgcctatctt gcggcaggcc
    11941 tgaaacgccc ctcgttctga ttgcttctcg tccggtcatg aaagctcccg ctttgccgac
    12001 aatatccgct cctccagaac ggccggccct gggcgacgcc gttcagggcg gacggcagtt
    12061 cgcgttcctg ctggtcgaca agttctcgat gttctcattg gcggcggcga tcgacacttt
    12121 tcgttcggcc aaccgcctgc ttggccgcga tttctacggc tggaccacgg tttccgccga
    12181 tggcgacgcc gtgatcgcct cgaacggcct gcccatcaag atcgactatg cggtcgccga
    12241 tatcccgccg gtcgacatca tcttcgtggc ggtcgggctg accaccgagt ttcccggcaa
    12301 gagcaaggtg ctgggtgcgc tgcgcagctg gggtcggcgt ggcggcgcgc ttggcgcgct
    12361 gtcggtcggc tcctacctcc tggccgaggc cggccagctg gaaggctgcc gctgcaccat
    12421 ccattgggaa aaccgcgccg gctttctcga acgctttccc gacatcaact gcaccggcaa
    12481 cgtcttcgag atcgaccgca agcgttatac ctgcgccggc ggcaccactt ccatcgacct
    12541 gatgctggag atcgtgcgcg gcgacctcgg ccccaatgtc gccaacggcg tcgccaacca
    12601 gttccagcat gaacgcatcc gctccgccgg cgaccgccag cgcgtcgggc cggaacgcga
    12661 ccttaccggc aagtcggaaa agctgcgccg catcgtcgaa ctgatggccg accatctcga
    12721 cgagccgctt tcggcggtgc aactggccaa gtcggccggg ctgtcggttc gccaggtcga
    12781 gcggctgttc ctgcgccacc tgacggtgac gcccggccgc tattacatgc ggctgaggct
    12841 ggagcgtgcg cgcgaattgc tgcgccagac caacatgccc atcctcgacg tggcgattgc
    12901 caccggcttc acatcgcact cctatttcgc ccagagctac cggttgcaat tcggccgtcc
    12961 gccttcggaa gagcggcgca caacctattg atcgtgaggg cgccggcgcg ccttgccagc
    13021 ccggcatcgc ctcgctagct tgccgctcca cgaacttccg caggagaatc catgacggcg
    13081 ctgctacgca acctgcttgg cgttttgttc ttgctgacga gcgccacggc gtcgctggcc
    13141 tccgggcagg tcatcgtcat cctcgacgca tcgggttcga tgtgggccca gatcgacggc
    13201 aggccgaaac aggagatcgc ccgcgaatcg ttgcgcaagg cgctggcatc ggtgccggcc
    13261 gatgcggaaa tcggctttat ggcttatggc cacagcgaca aggcaagctg cgatgatatc
    13321 gacctgatcg tttcaccggc agcaggcacc gcaagcgcca tttcaagcgc cgccgacaat
    13381 ctgaaattcc tgggcaagac gccgctgacc gccgcggtca aaaaggcggc cgaaaccttg
    13441 aaccacacgc aaggcgcttc gaagatcgtc ctgatcaccg atggcacgga aagctgcgaa
    13501 ggcgacccct gcgcgctcgg cagggaactc aaggccgccg gttcgggcct cattgtcgat
    13561 gtcgtcggct tcgggctttc ggcgcaggac ggaaaacagg tcgcctgcct cgcgcagagc
    13621 accggcggca aatatatcga ggcatccgac gccaaggcgc ttcaggcggc cttggttgaa
    13681 agcgtcgctg caacgcctgc ggcgcgggca acctctgccg cttccgcgca ggccacgccg
    13741 gaaacggcaa cgccgccaac cgccgaagcg gccaagcctg aattcaattt cctcccgacc
    13801 gtgacgttgg cgcagggcgg cgcgccgatt acgcaaggca atgcctggga aatatacaag
    13861 gccgccgcca acggcacgcg cggcgcatcg atcgccaccg aatacggggc ctatcaaggc
    13921 aatctggaac cgggcgatta catccttgtc gcccgcgacg gcgaagcgaa ggccgaacag
    13981 ccgctcaagg tcgaggcc
//
LOCUS       DASYCS010000178         3754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_40814, whole
            genome shotgun sequence.
ACCESSION   DASYCS010000178 DASYCS010000000
VERSION     DASYCS010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40814"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            337..2157
                     /locus_tag="VGM46_05750"
     CDS             337..2157
                     /locus_tag="VGM46_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006335847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY4192129.1"
                     /translation="MFVEREASGGETTSQERRDTAQADRRILGNVVQCDGARAVISAY
                     ADDSADPATGRWTVGKMISINLGATRTVGLVYAIDKPDRAWDFDGQNAIEVSVELVGE
                     VRDGAEPGSKLVFDRGITTYPHIGAVAHRIRNRDLQAVYDLAGRRAITIGSLSQDETI
                     DANIAIDDALARHFAVVGTTGVGKSTAVSLLLRKSIAARPDLRVLILDPHNEFAASLP
                     EYCIRLDSSTLDLPFWMFKLEEFAEVLFRGRDVVVEELDLLRDLIPIARNLYRSPGST
                     GYLRRGVDGLTADTPVPYRIADLVKQIDERIGLLESKADRPALKSLKIRIEQASADPR
                     YRFMFNSRLIEDTIHETIGKIFRVPHEGRPVTCLEMAGMPSEVVNSVCSVLARLAFDL
                     ALWSDGKLQMLLLCEEAHRYMPADPRLGFAPTRHALSRIAKEGRKYGCYLGIVTQRPG
                     ELDPTILSQCSTFFAMRLANEQDQAIVRSAIADSSASTLSFLSSMGQREAIAFGEGVA
                     TTMRMKFEILDRSLLPGSGKQDAPTPSEGGEVDLAALVGRLRSVAKPQSSPSVQPADQ
                     VRQPGDAGYRKPPAPVQPSDDFELRYGLKPATFGMRPQNS"
     gene            complement(2276..3067)
                     /locus_tag="VGM46_05755"
     CDS             complement(2276..3067)
                     /locus_tag="VGM46_05755"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006204942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY4192130.1"
                     /translation="MRFHKAESAFFVFIVVVLAAGLVTVHAYDYLQSVAVEFHRPFSI
                     AIIAYLNKLLFVTGVLLATALFFGMCFIYPIIRGQLKEEGKLRAMTVSLSARSETMEQ
                     AALTDGLTGMQNRRYFDDALKEYLHEFQRVGKPIGLMILDLDHFKQINDSYGHDVGDE
                     VLKAVANCLKDLTRYHDVAARLGGEEFGVVTPNMDFDLLNRFAERIRKAIAGISITSG
                     NIRLKVTTSVGLAVWDHKETAEEFYRRADRQLYEAKRMGRNRVCA"
     gene            complement(3179..>3754)
                     /locus_tag="VGM46_05760"
     CDS             complement(3179..>3754)
                     /locus_tag="VGM46_05760"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEY4192131.1"
                     /translation="LIEGISEMPEIPASVRDRWRYELREQGSARLHRILMENDASAAM
                     TLRASDGQRIVRALEVMEASGRSILDWQAAGGQPLVDLASAQLLVLEPDRPDLVQRIE
                     RRFERMMENDALQEVRALCALDLDRALPAMKAIGVRELKAAMDGETSFAEAIERAKIA
                     TRQYAKRQSTWFRHQFDVKWQRFRSADQVRI"
BASE COUNT          737 a         1221 c         1041 g          755 t
ORIGIN      
        1 tttcgcccgg cggttacacc gccttgccca cgcgctttcc tactacgaga atgatcttct
       61 tattcatggt cgcggacaat aagagcgaaa acggcaggcc gtcaatgggg cgaacgcaat
      121 ttgttgccgc cgcgtcaaca ccggctggtc tttcattgcc acggttgggt cgcagacggc
      181 aaaccgacag ccaatcaggc tgcggcgctg gcccttgcgg ctcatttgca tcgattctta
      241 gccagcatgt taggaatgcc tataccatcc cgccacacat caccttaacc ccgcgcctgt
      301 aagactaggc tgttaagggg aattgggtaa gcatatatgt tcgtcgaacg cgaggcatcc
      361 ggcggggaaa ccacgtcgca agagcgccgt gatacggccc aggcagacag gcgcattctc
      421 ggcaatgtcg tgcaatgcga cggcgcccgc gccgtcatca gcgcctatgc cgatgacagc
      481 gccgatcccg ccaccggccg gtggacggtc ggcaagatga tctcgatcaa tctgggcgcc
      541 acccgcaccg tgggcctggt ctacgccatc gacaagccgg atcgcgcctg ggatttcgac
      601 ggccagaacg ccatcgaggt cagcgtcgag cttgtcggcg aggttcgcga tggcgctgaa
      661 cccggctcta agctggtctt cgaccgtggc atcacgacct atccgcatat cggcgcggtg
      721 gcccaccgca tccgcaaccg cgacctccag gcggtctatg accttgccgg ccgtcgcgcg
      781 atcaccatcg gttcgctttc tcaagacgag accatcgacg ccaacatcgc catcgatgac
      841 gcgttggcgc gccacttcgc cgtggtcggc accaccggcg tcggcaagtc gaccgccgtc
      901 tcgttgctgc tgcgcaaatc catcgccgca cggcccgacc tgcgggtgct gatcctcgat
      961 ccgcacaatg aatttgcagc ttccctgcct gaatactgca tcaggctgga ttcctcgacg
     1021 ctcgacctgc ccttctggat gttcaagctg gaagagtttg ccgaagtttt gtttcgcggg
     1081 cgcgacgttg tcgtcgaaga actcgacctt cttcgtgacc tcattccaat cgccaggaac
     1141 ctttatcgca gccccggttc gacaggctat ttgcggcgtg gcgttgacgg cctgaccgcc
     1201 gatacgcccg tcccctaccg gattgccgat ctcgtcaaac agatcgatga acgcatcggc
     1261 ctgttggaaa gcaaggccga ccggccggcg ctcaaatccc tgaagatacg catcgagcag
     1321 gcgtctgccg atccgcgcta ccgtttcatg ttcaactccc ggctgatcga agacacgatc
     1381 catgaaacga tcggcaagat tttccgggtt ccccatgaag gtcgccccgt aacctgtctg
     1441 gagatggccg gaatgccgtc ggaggtggtc aattccgttt gctcggtgct cgcccggctg
     1501 gcgttcgacc ttgctctatg gagcgacggg aaactgcaaa tgcttctttt gtgcgaagaa
     1561 gcccaccgct atatgccggc cgatccacgc ctcggtttcg cgccgacccg ccatgcgctg
     1621 tcacgcatcg ccaaggaagg ccgcaaatat ggctgctatt tgggcatcgt cacccagcgc
     1681 cccggcgaac tcgacccgac catcctgtcg caatgctcga ctttcttcgc catgcggctg
     1741 gccaacgagc aggatcaggc gatcgttcgc tccgcaatcg ccgattcgtc ggcctcgacc
     1801 ctgtcgtttc tttcatcgat gggccaacgg gaagccatcg ccttcggtga aggcgtcgca
     1861 acgacgatgc ggatgaaatt tgaaatactg gaccggtccc tgctgccggg atcgggcaag
     1921 caggatgcac ccacaccctc cgagggcggc gaagtcgatc tcgccgcgct tgtcggccgt
     1981 ctgaggagcg tcgccaagcc gcaatcctcg cccagcgtcc aaccggcgga ccaggtccgc
     2041 cagcccggag atgccggcta cagaaagcca cccgcgccgg ttcagccgtc agacgatttc
     2101 gaactgcgct atggattgaa gcccgccaca ttcggaatgc ggccgcaaaa cagctgaagt
     2161 gggcttccac ggcgtttcgc gcgagcgcgg cgcatagccc gttatgcgct caggcgcaca
     2221 ccttccggcc gcatcttgag aaaattgatc tggcggcgca tagcccgtta tgcgctcagg
     2281 cgcacacccg gttccggccc atccgcttgg cttcatagag ttgccggtcg gcgcggcgat
     2341 aaaattcctc ggccgtttcc ttgtggtccc acaccgccag cccgacgctc gtcgtaacct
     2401 tcagccggat attgcccgag gtgatcgaga tgccggcaat cgccttgcgg atgcgctcgg
     2461 caaaccggtt gagcaggtcg aaatccatgt ttggcgtaac cacgccgaac tcctcgccgc
     2521 caaggcgcgc cgcgacatcg tgatagcgcg tcaaatcctt gaggcagttg gcgacagcct
     2581 tcagcacttc atcgcccacg tcgtgaccat aactgtcatt gatctgcttg aaatgatcga
     2641 ggtcgaggat catcagcccg atcggctttc caacccgctg gaactcgtgg agatattcct
     2701 tcagcgcgtc gtcaaagtag cgccggttct gcattccggt cagcccgtcg gtcagcgccg
     2761 cctgctccat cgtttcagag cgcgcgctca acgaaacggt catggcgcgc aatttcccct
     2821 cttccttcaa ctgcccgcga atgatcggat agatgaagca catcccaaaa aacagcgccg
     2881 ttgccagcaa cacccctgtc acgaagagaa gcttgttcag ataagcgatt atggcgatgc
     2941 tgaatggccg gtgaaattcc acggcgaccg attgcaggta gtcataggcg tgcacggtga
     3001 ctaggcctgc cgccaggact acgacgataa agacgaaaaa tgctgactcc gccttgtgaa
     3061 agcgcatgtc acccccggtg catattcggc gcgttatggc acgcgcgcgt taccgaatgt
     3121 tgaatcgatg caacgagcgc gtgtattttg acctccccgc attcactcct ggatgcagtc
     3181 agatccgaac ctgatcggca cttctaaagc gttgccattt cacatcgaat tgatgcctga
     3241 accaagtcga ttgccgcttg gcatattgcc ttgtggcgat cttggcgcgc tcgattgcct
     3301 cggcaaagga ggtttcgcca tccattgccg ccttcaattc acgcacgcct atggccttca
     3361 tcgccggcaa tgcccggtcg aggtcaagcg cgcaaagcgc cctgacctcc tgcaatgcgt
     3421 cattttccat catgcgctcg aagcgccgtt cgatgcgctg caccagatcg ggacggtccg
     3481 gctcaagcac cagcaattgc gcgctcgcca ggtccaccag cggctggccg cccgccgcct
     3541 gccaatcaag gattgagcgg ccggacgcct ccatcacctc aagtgcgcga acgatgcgct
     3601 ggccatcgct cgccctaagc gtcattgccg cactcgcatc gttttccatc agaatgcgat
     3661 gcaaccgtgc cgagccttgc tcgcgcaatt cgtaacgcca gcgatcacgc acagaagcgg
     3721 gaatttcagg catttcggaa atgccttcta tcag
//
LOCUS       DASYCS010000179         2870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_477633,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000179 DASYCS010000000
VERSION     DASYCS010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477633"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VGM46_05765"
     CDS             <1..289
                     /locus_tag="VGM46_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEY4192132.1"
                     /translation="GRNGGVKLGRSADSITLFDVVRVTEESFAMAECFESDATECPLI
                     DHCSLNAALREALNAFFAVLERHSIADLIAARSDVRSLLGIDFLASHAPAA"
     gene            complement(294..1085)
                     /locus_tag="VGM46_05770"
     CDS             complement(294..1085)
                     /locus_tag="VGM46_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HEY4192133.1"
                     /translation="MIEASDVSVAIGRKRIVHNIDFSARPGEITTMVGPNGSGKTTFL
                     KALSGELGYTGRISINGRDAASLKPFEMAAMRAVLPQATTLSFPFTVREIVKLGLVGG
                     RSGVLSGEAERLPERALARVDLEGFAGRFYQELSGGEQQRVQLARVLCQVWTPVLDRQ
                     PRFLLLDEPVSSLDIKHQLIIMRIAREFADGGGGVVAILHDLNLTAMVSDRIFVMHGG
                     SLAATGTPREVLQDHLLERVFDCRLVVGAVPANRIPFVLPQSADL"
     gene            complement(1093..2196)
                     /locus_tag="VGM46_05775"
     CDS             complement(1093..2196)
                     /locus_tag="VGM46_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006335372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEY4192134.1"
                     /translation="MADQTFAGRAGSMKAVAVGDRSGRARAVIVALALLLVATALFSL
                     ASGASDASVPGVIRGWLSGAVPGEAALGARDRLIVYDIRLPRVVMGALVGAALAVSGA
                     VMQGLFRNPLADPGLIGVSAGASLGAVAVIVLGATALAPLTVALGVLSLPLAAFFGGL
                     ATTLVLYRVATRHGQTSVATMLLAGIALAALAMALTGVLIFLADDRQLRDLTFWGLGS
                     LAGATWQKIGAVGPVIALALATTPFLARGLNALALGEATAGHLGIPVQRMKHTAIVAV
                     SAAVGASVAVSGGIGFVGIVVPHLLRLLIGPDNRYLLPASALLGASLLLIADALARTI
                     VSPAELPIGIVTALAGAPFFLWILLRKRGVLDL"
     gene            complement(2201..>2870)
                     /locus_tag="VGM46_05780"
     CDS             complement(2201..>2870)
                     /locus_tag="VGM46_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY4192135.1"
                     /translation="KLPDVGYMRQLSPEGVLSVDPSGILALQGSGPKEAIDVLKKASV
                     PYIEVPESFDRRGILDKIRIVGKALNVESKAATLAEAVDAKLAAAESQAASIRDRKRV
                     LFVLSMQGGKILASGSHTAANGIIQLAGGVNAIDGFSGYKQLSDEAVLTANPDVILMM
                     DSGDAPGSASDPFESPAIAATPAGKAHRLVRMDGGYLLGFGPRTAQAIHDLAVSIYGK
                     QAID"
BASE COUNT          560 a          983 c          895 g          432 t
ORIGIN      
        1 cgggcgcaat ggcggcgtaa agctggggcg cagcgccgat tccattaccc tgttcgatgt
       61 cgtgcgggtg acggaagaga gtttcgccat ggcggaatgc ttcgaatccg acgccaccga
      121 atgcccgttg atcgaccatt gctcgctcaa tgcggcgctg cgcgaggcgt tgaacgcttt
      181 cttcgcggtg ctggagcgtc actcgatcgc cgatctgata gccgcgcgtt ctgacgtgcg
      241 cagcttgctc ggcatcgatt tcctggcgtc acacgcgcct gcggcctaaa tcttcaaaga
      301 tcagccgact gaggcagcac gaacggtatc cggttggcgg gcacggcccc gacaaccagc
      361 cggcaatcga acactctttc cagaagatga tcttgcagca cctcccgcgg cgtaccggtc
      421 gcggcaagcg aaccgccatg catgacgaag atgcggtcgg aaaccatcgc cgtcaggttg
      481 aggtcgtgca ggatggccac caccccgccc ccgccatcgg caaattcgcg cgctatccgc
      541 atgatgataa gctggtgctt gatatcgagg ctggacaccg gctcatcgag gagaagaaag
      601 cgcggctgtc tgtccagaac cggcgtccat acctggcaaa gcacgcgcgc cagttgaacg
      661 cgctgttgct caccgcctga cagttcctga tagaatcggc cggcaaaccc ttccagatcg
      721 acgcgcgcca gcgcccgttc cggcaggcgc tccgcctcgc ctgaaagcac gccggaacgg
      781 ccgccgacca ggccgagctt gacgatttcg cgcacggtaa aagggaacga tagcgtcgtc
      841 gcctgtggca gcacggcccg catcgccgcc atctcgaagg gcttcagcga ggctgcgtca
      901 cggccattga tggaaatgcg ccccgtatag ccaagttcgc cggacagcgc tttcaggaag
      961 gtcgtcttgc ccgacccgtt cggcccgacc atggtggtta tttcaccggg ccgtgccgaa
     1021 aaatcgatat tatgaacgat ccgcttcctg ccgatcgcca ccgatacgtc gcttgcttcg
     1081 atcatcgccg cctcacaggt cgagaacgcc gcgcttgcgc agcaatatcc acaggaagaa
     1141 cggcgcgccg gccagcgcag tcacgatgcc gatcggcaat tccgccggcg aaacgatggt
     1201 gcgcgccagc gcatcggcaa tcagcagaag cgatgcgccc agcagcgccg aggccggcag
     1261 caggtagcgg ttgtccggac cgatcagcag ccgcagcaaa tgcggtacga cgatgccgac
     1321 gaacccgatg cccccgctga ccgcgacgga agccccgacc gccgccgaaa ccgccacgat
     1381 ggcggtatgt ttcatgcgct gcaccggaat gccgagatgg ccggccgtcg cctcgcccaa
     1441 agccagcgcg ttgaggcctc gcgccaggaa cggcgtcgtg gccagcgcca gcgcgataac
     1501 cggaccgacc gcgccgatct tctgccatgt cgcacccgcc agcgagccaa ggccccagaa
     1561 ggtcagatcg cgcaactgcc ggtcgtcggc caggaagatc agaacgccgg taagcgccat
     1621 ggccagagcg gccagcgcga tgccggcaag caacatcgtg gcgaccgatg tctgcccatg
     1681 ccgggtcgct acccggtaaa gcaccaatgt cgtcgccagc ccgccgaaaa aagcggccag
     1741 cggcagcgac agcacgccga gcgcgacggt cagcggcgca agcgccgttg cgcccagcac
     1801 gatcaccgcc acggcgccca gactggcgcc ggccgaaacg ccgataaggc cgggatcggc
     1861 cagcggattg cgaaacagcc cctgcatgac cgcgcctgaa accgccagcg ccgcccccac
     1921 cagcgcgccc atgacgacgc gcggcagacg aatgtcgtag acgatcagac ggtcgcgcgc
     1981 gccaagggcg gcctcgccgg gcacggctcc agacagccag ccgcgaatca cccccgggac
     2041 cgaagcatcc gacgcgccgg aagcgaggct gaacagcgcc gtcgcaacca gcagcagcgc
     2101 cagcgccacg atgacggcgc gagcgcgacc cgaccggtct ccgacggcta cagctttcat
     2161 cgagccggcg cgtccggcga atgtctgatc ggccatcgct ctagtcgatg gcctgcttgc
     2221 cgtagatcga gacggcgaga tcgtgaatgg cctgcgcggt gcgcgggccg aaaccaagca
     2281 gatagccgcc atccatgcgc accagccggt gcgccttgcc cgccggggtc gcggcaatcg
     2341 ccgggctttc gaacggatcg gaagcgctgc ccggcgcatc gccgctgtcc atcatcagga
     2401 tcacgtccgg attggcggtc agcacggcct cgtcggaaag ctgcttgtag ccggaaaagc
     2461 catcgatggc gttgacgccg ccggcaagct ggatgatgcc gttggccgcc gtgtggctgc
     2521 ccgaggcgag tatcttgccg ccttgcatgg acaacacgaa cagaacccgc ttgcggtccc
     2581 tgatcgaagc cgcctggctt tcagccgccg ccagcttggc atcgaccgcc tcggccagtg
     2641 tcgccgcctt gctttcaacg ttcaacgctt tgccgacgat gcgtatcttg tcgaggatgc
     2701 cgcgccggtc gaagctttcg ggaacctcaa tatagggaac gctggccttc ttgagcacgt
     2761 cgatggcttc cttcgggccg cttccctgca aggccagaat gccggaagga tcgaccgaaa
     2821 gcacgccctc cggcgaaagc tggcgcatat agccgacatc cggcagcttg
//
LOCUS       DASYCS010000180        29106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_767103,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000180 DASYCS010000000
VERSION     DASYCS010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 29106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29106
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767103"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="VGM46_05785"
     CDS             complement(<1..325)
                     /locus_tag="VGM46_05785"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY4192136.1"
                     /translation="MNTLLSGKGLVRRFGGLVAVNDVNVEVKAGEILGIIGPNGAGKT
                     TFFNLLSGFMKPTQGEVIFDGENVTGLRPYKLNRRGLARTFQLARPFSQLTVLENAMV
                     GSYRLS"
     gene            complement(322..1341)
                     /locus_tag="VGM46_05790"
     CDS             complement(322..1341)
                     /locus_tag="VGM46_05790"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY4192137.1"
                     /translation="MTALAANGARAGIRGSGAILPVLFLVVAAAAPIFANGYVLEVGI
                     VLLMFAYLGSSWNILGGWAGQMSFGHAAFFGLGAYVSALLWINAGVSPWIGMLIGALV
                     AAAFGVVVAIATFRAGLRGHYFALAMFAVAQMLMALFVNLKEVAGITIGGSEGLQLPY
                     TGTDPGQMVFDSRMPFYYILLAMLVVVQAVVKFISISRFGAYLQAVRNDEDAAEALGV
                     NVFGCKVAAMAVSAFFTGAVGAVYGQIYLYIEPGIVFSATYSIQGLLCAVVGGAGTVW
                     GPVLGALVLTPLAEVSKVFFRDISGLDLMIFGAVLIVFVRFIPQGLVGLWTETLARRR
                     GGSRS"
     gene            complement(1338..2204)
                     /locus_tag="VGM46_05795"
     CDS             complement(1338..2204)
                     /locus_tag="VGM46_05795"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY4192138.1"
                     /translation="MTSLIFEALVAGVLLGGVYGLIALGLNIIYGVLDIINFAHGAMM
                     MMGMYLTFWLFQVYGIDPYLSILIAGPVMFVFGALVYLFLLDPMRGKALQNQFLVTLG
                     LSLFITNAAQVLFSPDFRVLQPSYGQDVIQIGPAFVPLTRILTFVAALAATAVLLFIF
                     QRTRLGRSIRAVAQQQEGAALCGINVRLAYTLAFALGSACVGIAGAAVTPFFYVSPTV
                     GDLFNIASFVVVVLGGLGSMAGSLLGGLVLGITVSVGAALLPGSFKEVLMFAIFLGVL
                     LFRPTGLLGSKA"
     gene            complement(2302..3531)
                     /locus_tag="VGM46_05800"
     CDS             complement(2302..3531)
                     /locus_tag="VGM46_05800"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY4192139.1"
                     /translation="MSEIPQKFVINRRKVLLGGATLLAMPYVSRAYAEDEIIIANLHP
                     VTGFVALDGKVATAGCQAAVDEINEAGGIHSLGKKLRLITGDTQGKNDVASVETQRVI
                     EAGAVGLVGSYLSSTMRITTQVAEQKKTPHVADEGIADEILQRGFKYTFRLAPSSTSM
                     GQLSADNLVLLMKSAGLSTLSIAYMHEDSSFGSSVGRSFPGAAEKHGIEVRTVLSYAA
                     TSTDLSVEVSKLLATGADVVAASTYYGDGLLIAKLIQQRKPQIKAVVGVGSAAYANPS
                     FPKDMGAGANGYFNNIMHWNPASPAIPGLLERFAKFSGGLALTHQGAYAYSAVKVLAD
                     AIERAGSADKEAIREALTKTNLTDHILPQGPISFDEVGQNKNAGIVMLQVQNGAPAVV
                     LPDQYAQAKPIFPATFG"
     gene            complement(3714..4586)
                     /locus_tag="VGM46_05805"
     CDS             complement(3714..4586)
                     /locus_tag="VGM46_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY4192140.1"
                     /translation="MPTKVFTEVPAHLRKRQDGPVAGGRWQDGATPDCLIEGPCFDSD
                     GNLFLVDIPYGRIFRVSPQGEWTVIVEYDGEPNGLRRLPDGRLLVADYKQGILSLDPV
                     SGEMSSIVARYRSERLKGPNDLIVARNGDVYFTDQGQTGLHDPTGRVFRLRTNGQLDC
                     VLANGAGPNGLVLTADEKILYVAMTRDNAIWRVPILPDGTTMKVGRFCSYFGAVGPDG
                     MVIDSEGNIFVAHASLGCVFVHDRHGELIARIVSCRGRTVTNLTFGGPDRKTIFITES
                     ESGSVLTADWHVPG"
     gene            complement(4685..5461)
                     /locus_tag="VGM46_05810"
     CDS             complement(4685..5461)
                     /locus_tag="VGM46_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007562078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY4192141.1"
                     /translation="MDLRIDGLRVLVTAGASGIGLAIATALVEEGARVHVCDVDAKAL
                     SAAKAALPTVMTSVCDVSDRASVAALFDTQIKEFGGLDCLVNNAGIAGPTGRVDEISP
                     EDWESTLAVNITGQFNCVRLAVKHLAKSDNASIINLSSAAGRVGFGLRTPYAASKWAV
                     IGFTKSLSIELAELGIRANAILPGVVAGERQDRVLTEKAKAAGVSLDEMKRRALDRVS
                     VKEMISPQQLADTILFLVSPRARTTSGQAISVCGDLQSLS"
     gene            complement(5480..6172)
                     /locus_tag="VGM46_05815"
     CDS             complement(5480..6172)
                     /locus_tag="VGM46_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY4192142.1"
                     /translation="MKTALETIGQIERVTLGDRVHAELCDLLMAGKLFPGEKLSLRKV
                     AASIGVSMMPVREAVTRLVADEALTVLPNRAVSVPVMTQAKFQELTLVRCEIEGFATE
                     QAALRRTSADLKRIKSLDEQFRKAVNSEASNSDKALRLNKDLHFAIYEAAQLPVLVGI
                     IEGLWLKVGPVINLDLRSSSQRLRSGDAEQFHARIVSAIEAQDPAAARAALTADISGS
                     SQFIQSTGTLPI"
     gene            6297..7244
                     /locus_tag="VGM46_05820"
     CDS             6297..7244
                     /locus_tag="VGM46_05820"
                     /EC_number="1.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018259695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEY4192143.1"
                     /translation="MNSGEGRQVAVIGAGLIGRSWAIVFAAAGWSVRITDPDNVVLDA
                     APSLIRQSLTELSEYGLVENPHDAAARVTTAADMADAVANATIVQENGPEDVEVKRRI
                     FADLDRLSPPEAILASSSSAIPASQFTEGLPGRHRCLVAHPVNPPHLIPVVELCGAPW
                     TSAEVIATAREIFADIGQTPVTINSEIEGFVLNRLQGALLAEAFRLVGDQIISPQDLD
                     RTVKDGLGLRWSFMGPLQTIDLNAPGGIADYCKRYTGFYRRLSETPAPPEVWDAPNVE
                     RILQAWGDGHDSDARASQSAWRNGRLAALRAHKLAASKG"
     gene            7317..8246
                     /locus_tag="VGM46_05825"
     CDS             7317..8246
                     /locus_tag="VGM46_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015930257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEY4192144.1"
                     /translation="MAQRKVIITCAVTGAIHTPSMSPYLPITPHEIADAAVEAAEAGA
                     ALVHVHARDPVTGKPDQSPDAFTPILQAIKQRSNCVVNITTGGAPTMLVAERVKPAAH
                     FKPEVASLNMGSMNFGLYPMLSRFKTFKHDWEEPYLTGSVDRIFRNTFQDIEYILKTC
                     AENGTRFEIECYDIGHLYTLAHFAERGLIKPPFFVQSVFGLLGGIGPHPEDVAHMKRT
                     ADRLFGDQYKWSVLGAGRNQMPVAAMAAAMGGNVRVGLEDSLWIGPGQLAKSNAEQVR
                     AVRQIIEGLGLEIATSDEAREMLQLKGSDKVAF"
     gene            complement(8316..9362)
                     /locus_tag="VGM46_05830"
     CDS             complement(8316..9362)
                     /locus_tag="VGM46_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEY4192145.1"
                     /translation="MTARNLRWRNRPKGSNWGEFGADDQLGRLNFLTPEVVRKGLAEA
                     REGLAFCLSLPLDYPGGNVLSAARKPPVLRPTLRRGKPYFAYRMAEEDARTTDVVNDD
                     VAILHLQYSTHWDALGHVGSLFDADGDGEAEAVFYNGYRAETDFIGPTDAADAGAGEE
                     RTTFCAKALGAERMAERALQGRGVLVDLAHHYGRERAIIGYDALMKVMDTDKVAVEKG
                     DILCLHTGLGAAILEMNKEPTVEGLRSCAVLDGRDDRLLQWITDSNIAAIAADNYAVE
                     LNPGRDQCDCCAYLPLHEHCIFKLGMPLGELWYLTELAAWLRANGRSRFLLTAPPLRL
                     PGSVGTPLTPVATV"
     gene            complement(9447..10151)
                     /locus_tag="VGM46_05835"
     CDS             complement(9447..10151)
                     /locus_tag="VGM46_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4192146.1"
                     /translation="MLSVQGIEVRYGGIAAVRDVSFELAAGECLALIGPNGAGKSSTL
                     RAISGLAPVTAGRIAFLGDDMKGKPPHEVARLGVGHVPEGRHIFGPMTVEDNLLLGRQ
                     RLGGLSAQAGLEMVYGLLPKLQERRLQQAGSLSGGEQQMVVIGRAIIGEPKLLMLDEP
                     SMGLAPQIVEAVFELLAQLRSAGQTVLLVEQNAELALSFAQRCVVLSVGEVQLAGTSE
                     ELRTRQDIIDAYLGTF"
     gene            complement(10151..10912)
                     /locus_tag="VGM46_05840"
     CDS             complement(10151..10912)
                     /locus_tag="VGM46_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018263878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4192147.1"
                     /translation="MKLSVDKVGVRFGGLWANRDVTFGVREGSISAVIGPNGAGKTTL
                     FNVISGFRAPSQGEVRLDGEKVSGLPAERIARRGIGRTFQIPEICHDLSVIENVLVGA
                     YQHIKGGLLGQLTGLGGAIASEREARRRAEEELERTGLADLRDQSAKVLPLGKVRLLE
                     IARALASRPSLLLLDEAASGLNAREVGNLAELVRQLPGRGVTVLLIEHNVRFVMQLAE
                     TIAVLDHGQLLSVGTPEAIRNDERVITAYLGRRQG"
     gene            complement(10914..11879)
                     /locus_tag="VGM46_05845"
     CDS             complement(10914..11879)
                     /locus_tag="VGM46_05845"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY4192148.1"
                     /translation="MTMRRLIIPFVLLAWLVLPLVFTTDSTLGLMVFAAINLIQAIGL
                     SLLFGFAGQISLGQAGFFGIGAYVSAIAATRLGVPPLLGMVAATIVPAILAYLVGRPI
                     LRLKGYYLAMVTAGLGLIVHTVFVEWESMTGGYSGVANIPPLSIGSFAFDTPRAIYYF
                     ASGCAILVFVLAYRMIATSYGRAMRTTRESETAARSVGIDVSRLKAEVFALSAALSGF
                     SGALYAHYVGYISPDTFTIDASINLLLALVVGGINSLWGAAVGAILLTFLPEWLHQLQ
                     NAYGIVFGAIVIVLLTIEPGGLIGLAQRLIEGVRARRARPQPGAA"
     gene            complement(11883..12776)
                     /locus_tag="VGM46_05850"
     CDS             complement(11883..12776)
                     /locus_tag="VGM46_05850"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY4192149.1"
                     /translation="MDSAQLVQFAISGLTVGAIYALVGLGFNLIFNATDVINFAQGEF
                     VMLGGVLACIAIGEWHWPLWLAIIATVAAVGALGALIDFGAVSRVRNGSVMTIVMITL
                     GVSVVLKTATLLWVGPNPLYFDPFTAGPPLRVFGAVVQLQALWVIGIGGLAMAGLGIF
                     FSRSALGREMLACAIDREAASLMGIDVRKMVRISFILSGALGALAGVLITPLTNATYD
                     QGFTLALKGFAASALGGFGRSSSAIVGGLALGLIEAASVAVLPSGWKDSVALIVLILV
                     LMIKPSGLLGGRAERRHTQAM"
     gene            complement(12792..13928)
                     /locus_tag="VGM46_05855"
     CDS             complement(12792..13928)
                     /locus_tag="VGM46_05855"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY4192150.1"
                     /translation="MNGSAVLRSLVVAGSLLCLGSPLHAQETYKVGVSEPLSGGASSL
                     GIPVVDSVKIAADAINAKGGVNGHQIELVIRDDQSKPDIAVQNFRRLAEEGVYAIIGP
                     NQGSNMLAASAALQQAKIPACSFNNTISITKLGNPYIFRCQTSDADNTKAALTFARDK
                     LKAANVGILYTSDAYGSDAYAALQVEAKAMGLPITGAEKVNYGATDTTAEWTKLLATK
                     PAAIILWGSGSTMSIALRNGSQLGNTAPIIGSQGTAAAAIIKGAGAAAEGVYMVTLTA
                     PDQVTAGQTDLAKIYKDKNGDDYQLTIYDTIGWDALHLVVKAIENAKGDRNAMREGFE
                     QISKYELAAGTYTYTADNHEGLGVDSVWIVQVRDGRMVGVQHGF"
     gene            complement(14032..14784)
                     /locus_tag="VGM46_05860"
     CDS             complement(14032..14784)
                     /locus_tag="VGM46_05860"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEY4192151.1"
                     /translation="MKKKLRAGESLVGCFVMVPSPSITEMVAYAGFDFLILDTEHGSA
                     TPETLENQLRAAEAAGVPALVRTVGMTQGEILRIMDAGATGILVPHVCVPEEAASIAA
                     AAHYPPHGVRGLATTARAGRHGFTTLTEHLNKAYENTVVLVQIEDAKALDHVSAIAAL
                     DNIDGIFIGPADLSISLGHPGNPGHPTVVAAIDKVVKDTVAAGSVASTFVRSSAEVAD
                     VRKRGIPIAVFSSTSLFSQVLGETVRSAKSIA"
     gene            14993..15757
                     /locus_tag="VGM46_05865"
     CDS             14993..15757
                     /locus_tag="VGM46_05865"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEY4192152.1"
                     /translation="MKLFDLSERVAIVTGGNGGIGLGMAKGLCQAGAQTVLVGRNRDK
                     GEAAAGGLVGVGLKASFLEADVTDEASCRELVKAVTERHGGVDVLINNAGIAIRKSPQ
                     DYTLAEWHAVLDTNLTSAFLTSQAVYDSMKARGGGKIINIASVLALFGAPFAIAYSAS
                     KGGLVQFSKALATAWAPDNIQVNAILPGWIDTELTVEARAQVAGLAEKVIARVPAKRW
                     GKVEDFAGAAVFLASSASDFVTGSSLTVDGGFSAQG"
     gene            complement(15798..16868)
                     /locus_tag="VGM46_05870"
     CDS             complement(15798..16868)
                     /locus_tag="VGM46_05870"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY4192153.1"
                     /translation="MRRLILAFAAFSLLGTAARADDRQTKACPSPQQVQGFATCADIA
                     KAQEEGEVVLYSPAPEPGTVKLLEEFHRLFPKIKTSYVRLQSGALYARLMRERQSGSF
                     LADVLHLSDTGLVLDFQNHGGYGAYVSPQDSAYKSEYKSKPEGLWTWGAVIMAGIAYN
                     PKILAKDEAPKDWADLTNPRWAGKLSVKSSNSGLQLLAWDLLGKKLGDGFWPKIAELK
                     PTAFDSYVQQFDRIVNGDDEVAVTAQYSGYLLYKQKGAPIEMVLPPSGLPAGPEVWGV
                     IKEASHPEAARLFLDWFLSPLGQRIQADYVFLHSPRADVAPPPGGVPISELNLLLPTD
                     WSIYTPMRAGFVKTWNKMTGLR"
     gene            complement(16889..18016)
                     /locus_tag="VGM46_05875"
     CDS             complement(16889..18016)
                     /locus_tag="VGM46_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009451668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4192154.1"
                     /translation="MTPSGSQGSHAAGELGAQIDVTDLVIRYGAVTAVRGVSFSVGRG
                     EHLTLLGPSGCGKTTTLRSIAGLERPSGGEIIVDGKPFFSEAQRVDVPTEKRGISMVF
                     QSYAIWPHMSVFDNVAYGLNVRRQDKATIADAVRKALDLVQMTPYADRAASRLSGGQQ
                     QRVALARAIAFSPKVVLFDEPLSNLDAKLRNEMREELGNLRDRLKITSLYVTHDQEEA
                     FAISDKVVVMNAGRIEQIGTPETIYERPANHFVADFVGSANIVRGKVKGPNAVGSIAF
                     EANGVTVQALSPFQPDGTENEIAIRPAYVALEREKPQDARLNVLAATIVRRMFHGDFI
                     QYRVATPIGDLMVRGSPTNMFEPGSAVLACLKPEHCVLLKN"
     gene            complement(18013..19746)
                     /locus_tag="VGM46_05880"
     CDS             complement(18013..19746)
                     /locus_tag="VGM46_05880"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEY4192155.1"
                     /translation="MDKRARAPRWRLPVPGLRGQAFVTLLILVVCGFLVLYPVVFLVA
                     ESLNIGDPQIFPPEEIGLANYLESALDWTVIGNTLRVAFVATIISVTVGFVLAWILTR
                     TRIPGRGFLERLIEIPFYVTPLVGALAWAILAAPKSGFINQAWMYLGGSGPLVDIYTP
                     EGVAWVMALFEGTVAFVMIAAAMKSMDPSLEESSRVLGAGKFRTMMRVTLPLTFPAVM
                     GAFIFVFAEMLGSFAAALVLGIPGRFYVITTAIWELTLSYPPDYGRAAALGISLFAVM
                     FAMLALYRWVVRRGSYATISGKAFQPRAIDAGRSRWLLLGFCWLYIVIAVVLPLAALV
                     LTSFQRFATVLMSQSEFTLANYATAFSLDSIRSAIGNSLMLGFGVATVGVFVMMLLVW
                     IIYRSRAPGRGVMEYVVMFPQAVPRMVFGLALLWAWLNMPIPIYGTLWLLAIAYFTVL
                     LPLGVRTLAGVVLQIDRSLEECARVCGASWSQQLRTVTLPLMRPGMISAWVLLFIASV
                     RELGVSIYLMGPNSKVIAPAIVSSWSSSGTELTAAMAVIQTATVLIAVLVLFYISRRQ
                     SGQIIDKGGPL"
     gene            complement(19767..20837)
                     /locus_tag="VGM46_05885"
     CDS             complement(19767..20837)
                     /locus_tag="VGM46_05885"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY4192156.1"
                     /translation="MKFQVLFAATILAGGIQLARAADEPVVCASPQEVQGFKTCADVA
                     KAEQEGELVFYATDIEHGTVKVLGEFQKLFPKIKTSYIRLQAGALYARLMSERQGGSH
                     LADIFQIGDMGLVLDFQSKGGYAPYVSPELPAYKPEYRSAPEGYWTWAVLMPAGMAYN
                     PKIVPPDQAPKDWPDALDPKWDGAVSMKSANSGMQHVTWYELRSQMDGTYWDKMAAQK
                     PRAFDSMVQQFDRLVNGDDKIGLTAQYAAYLEYKHKGAPIEFIAPTSGLPASPEVVGV
                     VNTAPHPNAARLFLDWYLSPRGQKASVDYWYFHSPREDVAPPEGALPITAMHLLWPQD
                     WAAFAKSRTEFLRQMRSMTGMK"
     gene            complement(20901..21671)
                     /locus_tag="VGM46_05890"
     CDS             complement(20901..21671)
                     /locus_tag="VGM46_05890"
                     /inference="COORDINATES: protein motif:HMM:NF027439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY4192157.1"
                     /translation="MSAPEFQYLRTQVDGAVAVVTLNRPEVLNACNSATHREMQAAMD
                     WAEGDEQVKVVVITGAGRAFCSGSDLREVGQFQGVAARNYLKLDFSTKNRIAACSKPV
                     IGALHGHVAGGGFEMALACDIRIVADNVLFSLPEIVLGTIPGSGGLQRLPPIVGLGVA
                     KEWALTGRRIGAEEAHQRGLANAVHPLDRLMPEAMAFAQAMAERSSMALFLSKVAMDP
                     EPPQAHGMVGVYHMLASGACHDDPIYKARAGSYGKKDA"
     gene            complement(21668..22150)
                     /locus_tag="VGM46_05895"
     CDS             complement(21668..22150)
                     /locus_tag="VGM46_05895"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEY4192158.1"
                     /translation="MTLITMPEPVVLGLWFEDFETGKERFSPGRTITETDLVAFSGLT
                     GDYSQVHTDEEFCKKTEFGTRIAHGLLGLSIAQGLMWRTNYTQGTGVASLGWDKWTFK
                     KPIMIGDTVRVRWQLTQKRESGSKPNMGIITELVELMNQKGEVVQNGEHITLIRKRPK
                     "
     gene            complement(22147..23742)
                     /locus_tag="VGM46_05900"
     CDS             complement(22147..23742)
                     /locus_tag="VGM46_05900"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEY4192159.1"
                     /translation="MPKVLDAAQAVSLIPDGATVCVSGTGPVLEPDLVLKALEERFLA
                     ESHPRALNLITPMLPGDRPGEGGMNSFAHPGMLKRLVGASFARARHPRLLDLLRGGEV
                     EAYMISMGSMVELLTAIGSGKPGLFTPVGIGTFLDPRREGGALNAQSTRPPCRVEKVD
                     GKEYLFYPSFPIDVAIIRGTTADENGYISFEEETNTLGAVEIAMACKASGGRVIVQVK
                     RLARAGSLDPRLVRIPGPLVDVVVVNPHQTQLSPAMADPLNGWNPFLAGAMKHPLKGL
                     PTVKKGSERTILRRAALELREGDVVNLGAGVATRLPLVALEEGILDRVTFTNEHGVFG
                     GMMATALGGSFVPALNAEAIMDSVFQFNFYDGGGLDITFLGIGEVDGAGDLNVSRFGE
                     EINGPGGFNNITDRTKRIVFCGSLKSGGLKVEVAGGKTQIVQEGRHRKFVAKVEQVTF
                     NAARAIEKGQEVLYVTERGVMKLTAAGPELVEIAPGIDLERDIRAQIGFDLRVSDDLK
                     QMPAEIFADGIMNLEHAFGGNEE"
     gene            complement(23779..25323)
                     /locus_tag="VGM46_05905"
     CDS             complement(23779..25323)
                     /locus_tag="VGM46_05905"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEY4192160.1"
                     /translation="MEKRKSRIMSAEEAVAFVQSGSCLAVGGAGGVQEPDLLIEALVR
                     QFKETGSPAGLTEFHPIRTGEIEGRGTSLFGTAGLVRRMIGGSLWPVGVPELIRRINA
                     NEMEAYNFSIGVMYGLLEAASANRPGLITRVGLGTFEDPIHGGGALNSISREKLVQRI
                     EIDGEDFLYYRAIPVDTAFIRATTADELGNLTFEEEPAIIGALVLAQAAKANGGKVVA
                     QVKRVVAAGSLNPHMVRVPGALVDAVVVNPAQKQITNVVFDPTLVGAEQLDIASVPMR
                     PLDSAKVVMRRAFLEVRPDNLLAIGYGVPGFLPAIAIEEGVLDDVTFTVEHGVFGGVN
                     GYAAGGKTFPVAHNPTAIIDAADQIRFFAGGGIDCAFLGVGEVDASGNVNVGRFGDRI
                     PGSGGFVEMTQGIRKIVFCFVVGDRAGLKFTDQVQSISFNGQEAYRRGQHITYVTEKA
                     VFELRENGLTLTEIAPGVDPRRDLLDAMPCRIEVAETLRKMPAMCFAPGPMNVRRLWS
                     ESRPAQ"
     gene            complement(25323..26081)
                     /locus_tag="VGM46_05910"
     CDS             complement(25323..26081)
                     /locus_tag="VGM46_05910"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY4192161.1"
                     /translation="MADRVKLAVEGRVAVLTLARPDVLNAIDRAMCDAIIAALDEIEA
                     QEQASVLVIAGEGRSFCAGADLKHMRTLAGEMLRRFIERTWIVGERINRSPLLSIAAV
                     HGFALGGGAELALGCDMRLVEKTAAFGFPEMTLGSVPGSGGMQRLHALVGPSRALELV
                     MEGTRVAGPRAVEIGLANRVVEDGRALPEALEWARALSQRPAEAIRYAKTCIRLPADG
                     AIAPMVQGLVSSVCQSAKDYRANAGSFLEDKGQA"
     gene            complement(26084..27820)
                     /locus_tag="VGM46_05915"
     CDS             complement(26084..27820)
                     /locus_tag="VGM46_05915"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY4192162.1"
                     /translation="MNFGAMEELTADVVVVGAGGAGLRVAYEAAAAGARTILITKGRI
                     AVSGATAVGLASTAGFAVPDGAGDPLDNPDVHYNDIMTAAQGCADPALVRILVDEALE
                     AAEDLDRWNIDFIREEATGKPLVAQGDFASRPRNRKIYHHGRPITVALKGETARLGVH
                     SLEGTTVLSLLQDEKGVHGLVALTRQGQLIRVKAGATVLTTGGAGQLFKLSLMPPDIT
                     GDGYALGYRAGATLANMEFMQAGFGTVKPALNIVYTWFWGGVPPFVDREGRPVLDGAL
                     PAGTSTADAMLKKVEHYPFSASDASRWLEIATKNAMRDGRADETGSFFLDLRTTDPDK
                     LTQKGFTQLWNVSKGWLRQKHMDIDAAPLRIGLFGHAINGGLVIDEHGQTSVEGLLSA
                     GENAAGPYGADRLGGNMLLNCQVFGKRAGRRAAEIGRARAAANVERLAEAAVADLQAK
                     TGGDGTLKIRQAHQRIKQTMTDHALVVRNEKGLKTAEAELAELAAALEAGQFGIETVK
                     DVVAFTEAVNMIDVGRAMCAAAIERRETRGSHYREDADFRDDAKWSCPIMVRHVGNGA
                     AKAEPGRFGGAK"
     gene            complement(27817..28965)
                     /locus_tag="VGM46_05920"
     CDS             complement(27817..28965)
                     /locus_tag="VGM46_05920"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY4192163.1"
                     /translation="MRQLRDAVRAFVDDIVEPHAAEIDRTNEIPEHILDKARELGLFG
                     FSIPEAYGGLGESELASSVALETMSYGPGGVTFFIAPSAPAAAINLVGRQDQKERFLP
                     DLASGSRYAAFCLSEAGAGSDAAGIKTRAVRKNGRWSISGTKLWISRATKAGVFLVSA
                     VTDPEKGAKGGITVFLIDKRKGINVGKPEWQLGGRGSGSAEVNFDEVEASDDEMLGEV
                     GFGFSALKFILGRARLWAAARAVGVIGRSLELSLVHAENRVQFGKKIGEFQAIKHKIA
                     DMSADLYTARLLLYQAAKLFDEGHDAAQEAAYAKLFCSEAAGRAADAAVQVHGAMGVS
                     QDFGVERLFRDCRSYRILDGTSDIQRGMIASRVQRVGLGDTLAPGGLA"
BASE COUNT         5333 a         9596 c         9153 g         5024 t
ORIGIN      
        1 gcgacaggcg atagctgccg accatggcgt tctccaggac cgtcagttgg gaaaacggcc
       61 gggccagctg gaaagtgcgc gcaagcccgc gtcggttgag cttgtagggg cgtagcccgg
      121 tgacgttctc gccatcgaag atgacctcgc cctgcgtagg cttcatgaag cccgacaaca
      181 ggttgaagaa ggtcgtcttg ccggcgccgt tcggcccgat gatgccgagt atctcgccgg
      241 ccttcacctc gacattgacg tcgttgacgg caaccaggcc gccgaaacgg cgcaccagcc
      301 ccttgccgga aagcagcgtg ttcatgaccg gctccctccc ctgcgcctgg cgagtgtctc
      361 ggtccacagg ccgacaaggc cttgcgggat gaagcggacg aagacgatca gcaccgcgcc
      421 gaagatcatc aggtcgaggc cggatatatc gcggaagaac accttggaca cctcggccag
      481 cggcgtaagc acgagcgccc ccagcaccgg cccccagacg gttcccgccc cgcctaccac
      541 ggcgcaaagc aggccctgga tggaataggt cgccgaaaag acgatgcccg gctcgatata
      601 gagatagatt tggccataga ccgcgccgac cgcgccggtg aagaacgcgc tgacggccat
      661 tgcggccacc ttgcagccga acacgttgac gccaagcgcc tccgccgcat cctcgtcgtt
      721 tcgcacggcc tgcaaatagg ccccgaaccg gctgatcgag atgaacttca ccaccgcctg
      781 caccacgacc agcatggcaa gcaggatgta gtaaaacggc atgcggctgt cgaagaccat
      841 ctggccgggg tcggttcccg tatacggaag ttgcagcccc tcggagccgc cgatggtgat
      901 gccggcgacc tccttgagat tgacgaacag cgccatcagc atctgcgcca ccgcaaacat
      961 cgccagcgcg aaatagtggc cgcgcagccc ggcccggaag gtggcgatgg cgacgacgac
     1021 gccgaaggcc gccgcgacca gcgccccgat cagcatgccg atccacgggg agacgccggc
     1081 attgatccac aaaagagcag aaacataagc accaagcccg aaaaacgcgg catggccgaa
     1141 cgacatctgc cctgcccagc cgcccagtat gttccacgag cttcccagat aggcgaacat
     1201 caggaggacg atgccgacct ccagcacgta gccattcgcg aaaatggggg cggccgccgc
     1261 cacgaccagg aacagcaccg gcaggatcgc gccgctgccc cggatgccgg cgcgcgcgcc
     1321 gtttgccgcg agcgcggtca cgccttgctc cccaggagac cggtgggacg gaagagaagc
     1381 acgccgagga atatggcgaa catcagcacc tccttgaaag aaccaggcaa aagggcagcg
     1441 cccaccgaaa ccgttattcc gagcaccagc ccgccgagca gcgacccggc catgctgcca
     1501 agcccgccca gcaccacgac gacgaaggag gcgatgttga aaagatcgcc cactgtcggc
     1561 gagacgtaga agaagggcgt gacggcggcg ccggcaatgc cgacgcaggc gctgccgagc
     1621 gcgaaggcca gcgtataggc cagccgcaca ttgatgccgc acagcgccgc cccttcctgc
     1681 tgctgggcga ccgcgcgaat ggagcggcca agccgcgtgc gctggaagat gaacagcagc
     1741 acggccgtcg ccgccagcgc ggcgacgaag gtcaagatgc gcgtcagcgg cacgaaagcc
     1801 gggccgatct ggatgacatc ctggccataa cttggctgca aaaccctgaa atcgggtgaa
     1861 aaaagcacct gcgcggcatt ggttatgaac agcgacaggc caagcgtcac caggaactgg
     1921 ttctgcaacg ccttgccgcg catcggatcg agcaggaaca gatagaccag cgcgccgaag
     1981 acgaacatga caggcccggc aatgaggatg ctcagataag ggtcgatgcc atagacctgg
     2041 aacagccaga aagtcaggta catgcccatc atcatcatcg cgccatgggc gaaattgatg
     2101 atgtcgagca cgccatagat gatgttgagg ccgagcgcga tcagcccata gacgccgcca
     2161 agcaggacgc cggcgacgag cgcctcgaag ataagactgg tcactgttcc tcctcgatgt
     2221 caggcgagcc gcaggcatgg gacaggccca tgcccaagag caccccggcc ggacaagccc
     2281 ggccggcgcg ctgctgcggg ttcagccgaa ggtggccggg aatatcggct tggcctgcgc
     2341 atactgatcg ggcaggacaa ccgccggcgc gccgttctgc acctggagca tgacgatgcc
     2401 ggcattcttg ttctggccga cttcatcgaa ggaaatcggg ccttgcggca ggatgtggtc
     2461 ggtgaggttg gttttcgtca gcgcctcgcg gatcgcttcc ttgtctgccg agccggcacg
     2521 ctcgatcgca tcggcaagca ccttgaccgc cgaataggcg taggcgccct ggtgggtcag
     2581 cgccagcccg ccgctgaact tggcgaaccg ttccagcagg ccggggatcg ccggcgacgc
     2641 cgggttccag tgcatgatgt tgttgaaata gccgttcgcg cccgccccca tgtccttcgg
     2701 aaaggatggg ttggcatagg cggcgctgcc gacgcccacg acagccttga tctgcggctt
     2761 gcgctgctgg atcagcttgg cgatcagcag gccgtcgcca taataggtgc tcgccgccac
     2821 gacatcggcg ccggtggcca gaagcttgga aacctcgacg ctgagatcgg tggaggtggc
     2881 ggcgtaggac aggacggtgc gcacttcgat gccgtgcttt tcggccgcgc ccggaaacga
     2941 gcgcccgacc gacgagccaa aggacgaatc ctcgtgcatg taggcgatgc tcaaggtcga
     3001 aaggcccgcg ctcttcatca gcaggaccag gttgtcggcg gaaagctggc ccatgctggt
     3061 cgaggacggc gccaagcgga acgtgtattt gaacccgcgc tggaggattt cgtcggcaat
     3121 gccctcgtcg gccacatgcg gcgtcttctt ctgctcggcc acctgcgtgg tgatgcgcat
     3181 cgtcgaactg aggtaggagc cgaccaggcc gacggcgccg gcctcgatga cgcgctgcgt
     3241 ttccacgctg gcgacgtcgt tcttgccctg cgtgtcgccc gtgatcagtc gaagcttctt
     3301 gcccagcgaa tggatgccgc cggcctcgtt gatttcatcg acggccgcct ggcagccggc
     3361 ggtcgccacc ttgccgtcca gcgccacgaa acccgtcacc ggatgcagat tggcgatgat
     3421 gatctcgtcc tccgcatagg cgcggctgac gtaaggcatg gccagaaggg tcgcgccgcc
     3481 aagcaggacc ttgcggcgat tgatgacgaa tttctgcggg atttcgctca tttggttctc
     3541 ctccgctgtc tgccggccac atttcctgtg gccggcactc ctcagtcgcc ggttcgtgcc
     3601 ggcgcagttt tacgtgatct gagatcacag ttaacttgac agtgccgcgc gcccaatgca
     3661 agcggcattt tcagtagtcc atagagccgg cgtcgggacg ccggctcgcc ctgttatccc
     3721 ggcacatgcc agtcggcggt caagaccgag ccgctctcgg attcggtgat gaagatcgtc
     3781 ttgcgatccg gcccgccgaa ggtcaggttg gtgacggttc ggccccggca ggaaacgatc
     3841 cgcgcgatca actcgccatg cctgtcatgc acgaacacgc agccgagcga ggcgtgggcg
     3901 acgaagatgt tgccttcgct gtcgatgacc attccgtcag ggccgaccgc gccgaaatag
     3961 ctacagaagc gccccacctt catggtcgtg ccatcgggaa ggatcggcac gcgccagatg
     4021 gcgttgtcgc gcgtcatcgc cacatagagg atcttctcgt cggcggtcag gacaaggccg
     4081 ttggggccgg ccccgttggc cagcacgcaa tccagctggc cgttcgtgcg cagccggaag
     4141 acacggcctg tcggatcgtg cagcccggtc tggccctggt cggtgaaata gacatcgcca
     4201 ttgcgcgcca cgatcaggtc gttggggcct ttcagccgct cgctgcgata gcgtgcgaca
     4261 attgacgaca tctcgccgct gaccggatcg agggaaagaa tgccctgctt gtaatcggcc
     4321 accagcagcc tgccgtccgg caaccgccgc aagccattcg gctcgccgtc atattcgacg
     4381 atcaccgtcc actcgccctg cggcgagacc cggaatatgc gcccgtaagg aatatcgacg
     4441 agaaagagat ttccgtcgct gtcgaagcag gggccttcga tgaggcagtc cggcgtcgcg
     4501 ccgtcctgcc agcggccacc ggcgacgggg ccgtcctgcc gcttgcgcag atgggcggga
     4561 acctcggtaa aaaccttggt cggcactatt cttggggggt gatcgagaaa caagctgcgc
     4621 tccaactatc gaggccatgt ctttacggcc gcagccttgc ggaaaggcgg cggcgacatc
     4681 gcggtcagga caatgactga aggtctccgc aaacgctgat cgcctggccg gaagtggtgc
     4741 gcgcacgcgg cgaaaccaga aacaggatgg tatcggccaa ctgctgcggc gaaatcatct
     4801 ccttcaccga cacgcggtcc agcgcccggc gcttcatctc gtccagcgag acgccggcgg
     4861 ccttggcctt ttccgtcagc acgcggtcct ggcgctcgcc cgccacgacg cccggcaaaa
     4921 tggcgttggc gcggatgcca agctccgcaa gctcgatgga cagcgatttc gtgaagccga
     4981 tgacggccca tttcgaggcg gcataagggg tgcgcaggcc gaaccccacc cgcccggccg
     5041 ccgaagacag gttgatgatg ctggcattgt cgctcttggc gagatgcttc accgcaagcc
     5101 gcacgcaatt gaattgaccc gtaatgttga cggccagcgt gctttcccag tcttcgggcg
     5161 agatttcgtc cacgcgaccc gtcggcccgg ctatgccggc attgttgacg aggcagtcca
     5221 gcccgccgaa ttccttgatc tgcgtgtcga acaaagccgc caccgaagcg cggtcggaaa
     5281 cgtcgcagac gctggtcatg acagttggca gggcagcctt ggcggcgctc agcgccttgg
     5341 cgtcgacgtc gcagacatgg acccttgcac cttcttcgac gagggcggtc gcgatggcca
     5401 ggcctatgcc gcttgcgccg gccgtgacga gtacacgaag cccatcgatc ctcaagtcca
     5461 taacctgctg tcctgctttc tagatcggca aggtgccggt cgactggatg aattgcgacg
     5521 aaccggaaat gtccgccgtc agcgccgcgc gagcggcggc cgggtcctgc gcctcgatcg
     5581 ccgagacgat gcgggcatgg aactgctcgg cgtcgccgga acgaagacgc tgcgacgacg
     5641 agcgcagatc gagattgatg accgggccga ccttcagcca aagcccctct atgatgccga
     5701 cgagcaccgg caactgcgcc gcctcgtaga tggcgaagtg cagatccttg ttgagcctca
     5761 gcgccttgtc ggaattggac gcctcggagt tgaccgcctt gcggaactgc tcgtcgagcg
     5821 acttgatcct tttcaggtcc gcgcttgtgc gccgaagtgc cgcctgctcg gtggcgaagc
     5881 cttcgatctc gcagcgcacc agcgtcagtt cctggaattt cgcctgcgtc atcaccggaa
     5941 cgctgaccgc gcggttgggc agcacggtca gcgcctcgtc ggccaccagc cgcgttaccg
     6001 cttcgcgcac cggcatcatc gaaacgccga tagacgcggc gactttacgc agcgaaagct
     6061 tctcgcccgg aaacagcttc ccggccatca gcaggtcgca gagttcggcg tgaacacggt
     6121 cgcccaatgt cacgcgttcg atttgtccga tcgtctcaag tgccgttttc atgtccaatc
     6181 ctctcctata gctctggaca aattgccgtc atgatgcaac ctatttcgtg atcacagatc
     6241 acggattttg attatttagt gttgaaaatg ccgggacata aggacaggtg caggccatga
     6301 actcaggcga gggaaggcaa gttgcggtta ttggcgccgg tttgatcggc cgctcatggg
     6361 cgattgtgtt cgccgcggca ggatggagcg tgcgaatcac cgatccggac aatgtggtgc
     6421 ttgacgcagc gccttcgctc atccgccaaa gcctcacgga actttccgaa tacgggcttg
     6481 tggaaaaccc gcacgatgcc gctgcacgcg tgacgacggc cgccgacatg gccgacgccg
     6541 tggcgaacgc cactatcgtg caggaaaacg gccccgagga tgtcgaggtg aagcggcgga
     6601 tattcgccga tctcgaccgg ctgtcgccgc cagaggctat cctggcctca tcgtcttccg
     6661 ccattcccgc ctcccagttc accgaaggcc tgcctgggcg gcatcgctgc cttgtcgcgc
     6721 atccggtcaa tccccctcat ctcattcccg tcgtggaact ctgcggcgct ccgtggactt
     6781 cggccgaggt catcgccacc gcccgcgaga tttttgccga tattggacag actcccgtca
     6841 ctataaacag cgaaatcgaa ggctttgtct tgaatcgtct tcagggagcc ttgctggccg
     6901 aagctttccg gctcgtcggc gaccagatta tcagcccgca agacctcgac aggaccgtca
     6961 aggacggtct tggcctgcgc tggagcttca tggggcctct ccagacgatc gacctgaacg
     7021 ccccgggcgg aattgccgac tactgcaaac gctataccgg gttttaccgg cgcctgagcg
     7081 aaacgcccgc gccgcccgaa gtgtgggatg cgccgaatgt cgagcgcatc ctccaagcct
     7141 ggggcgatgg ccacgacagc gacgcgcgcg cctcgcaaag cgcctggcgc aacggaaggc
     7201 tggcggcctt gcgcgcgcac aagctcgccg cgtcaaaggg ctgatccggg cagccaggca
     7261 tgacgcgatt caccttaatc ctcccaccca tccaccttca ttaagcgagg cttccaatgg
     7321 cacagcgaaa agtcatcatc acctgcgcgg taaccggggc gatccacaca ccctccatgt
     7381 cgccctatct gccgataacc ccgcatgaaa tcgccgatgc ggcggtagag gccgccgagg
     7441 ccggcgccgc ccttgtccat gtgcatgccc gcgatccggt aacgggaaag ccggaccaaa
     7501 gcccggatgc gtttacgccg atcctccagg cgatcaagca gcgctcgaac tgcgtcgtga
     7561 acatcaccac cggcggcgcg ccgacgatgc tggtcgccga acgcgtcaag cctgccgccc
     7621 atttcaagcc cgaggttgct tcgctcaaca tggggtcgat gaatttcggc ctctatccga
     7681 tgctgtcgcg cttcaagacc ttcaagcatg actgggaaga gccttacctg accggctccg
     7741 tcgaccgcat cttccgcaac accttccagg acatcgaata tatcctcaag acctgcgccg
     7801 aaaacggcac gcgctttgag atcgaatgct atgatatcgg ccatctctac acgctggcgc
     7861 acttcgccga gcgtggcctg atcaagccgc ccttcttcgt gcagagcgtc ttcggcctgc
     7921 tgggcggcat cggcccgcac ccggaagacg tggcgcacat gaagcgcacg gccgaccgcc
     7981 tgttcggcga ccaatacaaa tggtctgtgc tcggcgccgg gcgcaaccag atgccggtcg
     8041 ccgcaatggc ggcagcgatg ggcggcaatg tccgtgtcgg cctggaggat tcgctgtgga
     8101 tcgggccggg ccagcttgcg aagtccaacg ccgagcaggt ccgcgccgtg cgccagatca
     8161 tcgaaggtct cggcctcgaa atcgcgacat cggacgaggc gcgcgaaatg ctgcaactca
     8221 aggggtccga caaggtcgca ttctgatttt tccgatgacg gaacatcggc cggacatgcg
     8281 gccgatgttc cgtcagcaag gcacgattgc cgcgttcaaa ccgttgccac cggcgtcagc
     8341 ggagtgccga ccgaccccgg caggcgcagc ggcggcgcgg tcaagaggaa gcggcttcgc
     8401 ccgttagccc gcagccatgc ggccagttcg gtcagatacc acaactcgcc aaggggcatg
     8461 ccgagcttga agatgcagtg ctcgtgcagt ggcaggtagg cgcagcaatc gcactggtcg
     8521 cgtccgggat tgagttccac cgcataatta tccgcggcaa tggcggcgat attgctgtcg
     8581 gtgatccatt gcagcagcct gtcgtcgcgc ccgtcgagca cggcgcagct gcgcagcccc
     8641 tcgacggtcg gctccttgtt catttcgaga atggcggcgc caaggccggt atgcaggcac
     8701 aatatatcgc ccttctcgac ggccaccttg tcggtgtcca tcaccttcat cagcgcatca
     8761 tatccgatga tcgcccgttc gcgcccgtaa tgatgcgcga gatcgaccag cacgccgcgt
     8821 ccctgcaagg cgcgttcggc catgcgctcc gcgcccagcg ccttggcgca gaatgtggtg
     8881 cgctcctcac ccgcgccggc atcggcggcg tcggtcgggc cgatgaaatc cgtttcggcg
     8941 cggtatccat tgtagaaaac ggcttccgcc tcgccgtcgc catcggcgtc gaacagcgag
     9001 ccgacatggc ccagcgcgtc ccaatgcgtc gaatattgca gatgcaggat cgcaacatcg
     9061 tcgttgacca catcggtcgt gcgggcgtct tcttccgcca tgcgataggc gaaatagggt
     9121 tttccccgcc gcagggtcgg ccgcaggacg ggcggcttgc gcgcggcgct gagcacgttt
     9181 ccgcccggat aatcgagcgg caggctgagg cagaaggcaa ggccttcgcg ggcttcggca
     9241 agccccttgc gcaccacctc cggggtcagg aaattcagcc ggccaagctg gtcgtcagcg
     9301 ccgaattcgc cccagttgga ccctttcggc cggttgcgcc agcgaaggtt gcgcgccgtc
     9361 atgcatcggt ctcctggcgg ttggacgcgg ccccggcact ttgccggatg gacagcgtcg
     9421 gcatcgaaat gctcgtcaaa ggcacgctag aacgtaccga gataggcgtc gataatatcc
     9481 tggcgcgtgc gcagttcctc ggaggttccg gcaagctgca cctcgccgac gctcagcacc
     9541 acgcatcgct gcgcgaaact cagcgccagt tcggcattct gctcgaccag cagcaccgtc
     9601 tggccggcgc tgcgcaattg ggccagcagt tcgaacaccg cttccacgat ctgcggggcc
     9661 agccccatcg aaggctcgtc cagcatcaaa agcttaggct cgccgatgat ggcgcggccg
     9721 atgaccacca tctgctgttc gccgcccgaa aggcttcccg cctgctgcaa gcggcgttcc
     9781 tggagcttcg gcaacaggcc gtagaccatt tcgaggcccg cctgcgccga cagtccgccg
     9841 agccgctggc ggccaaggag cagattgtcc tccaccgtca tcgggccgaa tatatgccgc
     9901 ccttccggaa cgtggccgac acccagcctt gccacctcat gcggcggctt tcccttcatg
     9961 tcgtctccaa ggaaggcgat cctgcctgcc gtcaccggcg ccaggcccga tatggcccgc
    10021 agcgtcgagg acttgcctgc gccgttcgga ccgatcagcg ccagacattc gccggccgca
    10081 agttcgaacg acacgtcgcg caccgccgcg atgccgccat accgcacctc aatgccctgt
    10141 acgctcagca tcagccctgc cgcctcccaa gataagcggt gatcacgcgc tcgtcgttcc
    10201 tgatggcttc gggcgtgccg acgctgagca attgcccgtg gtcgagcacc gcgatcgttt
    10261 cagccagctg catgacgaag cgcacattat gctcgatcag caggacggtg acgccccggc
    10321 ccggtaactg ccgcaccagt tcggccaggt tgccgacctc gcgcgcattg aggccggagg
    10381 cggcctcgtc cagaagcaaa aggcttggcc gcgaagccag cgcgcgcgct atctccagca
    10441 gcctgacctt gcccagcggc aggaccttgg ccgactggtc gcgcaagtcg gcaagacctg
    10501 tcctttccaa ttcctcctcg gcgcgacggc gcgcttcccg ttcgctggca atcgcgccgc
    10561 caagcccggt gagctggccg agcaggccgc ccttgatgtg ctggtaagcc ccgaccagca
    10621 cgttctcgat caccgagaga tcgtggcaga tctccggtat ctggaacgtg cgcccgatgc
    10681 cgcgccgcgc gatgcgctcg gccggcaggc cggaaacctt ttcgccgtcg agccgcactt
    10741 cgccctgcga gggcgcccgg aaaccggaga tgacgttgaa cagcgtggtc ttgccggccc
    10801 cgttcgggcc gatcaccgcg cttatcgagc cttcgcggac accgaatgta acatcgcggt
    10861 tggcccacag gccgccgaac cgcactccca ccttgtcgac ggacaacttc atgtcaggca
    10921 gcaccgggtt ggggccgcgc gcgacgcgcg cgcacgcctt cgatcagccg ctgcgccagg
    10981 ccgatcagcc cgccgggttc gatggtcagc agtacgatga cgatggctcc gaaaacgatg
    11041 ccataggcat tctggagctg gtgaagccat tcaggcagga aggtgagcag gatggccccg
    11101 accgccgcgc cccacagcga gttgatgccg ccgacgacca gcgccagcaa caggttgatc
    11161 gaggcgtcga tggtgaaggt gtcggggctg atatagccga cataatgcgc atagagcgcg
    11221 cccgaaaagc ctgacagcgc cgccgaaagg gcaaacacct ccgccttcag ccgcgagacg
    11281 tcgatgccga ccgaacgcgc cgccgtctcg gattcgcgcg tcgtgcgcat ggcgcggcca
    11341 tagctcgtcg cgatcatgcg gtaggcaagc acgaagacca ggatcgcgca gcccgacgcg
    11401 aaataataga tggcgcgcgg cgtatcgaag gcgaacgagc cgatggacag cggcgggatg
    11461 ttggccacgc cggaatagcc gccggtcatg ctctcccact ccacgaagac ggtgtggacg
    11521 atgaggccga ggccggccgt caccatggcg aggtaatagc ccttgagacg caggatcggc
    11581 cgaccgacca gataggccag gatggccgga acgatggtgg ccgcgaccat tcccagcaaa
    11641 ggcggcacgc cgagccgcgt ggctgcaata gccgagacgt aagcgccgat gccgaagaag
    11701 cccgcctgcc ccagcgatat ctggccggca aagccgaaca gcagggaaag gccgatggcc
    11761 tgaatgaggt tgatggcggc gaacaccatc aggccgagcg tcgaatccgt ggtgaagacc
    11821 agcggcagga cgagccaggc cagcagcaca aacgggatga tcaggcggcg catggtcatg
    11881 gcctacatcg cctgcgtatg gcggcgttcc gcccggccgc cgagcagccc cgatggcttg
    11941 atcatcaaaa cgaggatgag cacgatcagc gccaccgaat ccttccagcc cgaaggcagc
    12001 accgcaaccg acgccgcctc gatgaggcca agcgccaggc cgccgacgat tgccgagctg
    12061 ctgcggccga aaccgcccag cgccgaggcc gcgaaacctt tgagggcgag cgtgaagccc
    12121 tggtcatagg tcgcgttggt caagggcgtg atcagcacgc cggccagcgc gccgagcgcg
    12181 ccggacagga tgaagctgat gcgcaccatc ttgcgcacgt cgatgcccat cagcgaggcc
    12241 gcctcgcggt cgatggcgca ggcgagcatt tcgcgcccca gcgccgagcg gctgaagaaa
    12301 atgccaagcc ctgccatggc caggccgccg atgccgatca cccacagcgc ctgcaactgc
    12361 accaccgcgc cgaagacccg cagcggcggc ccggcggtga agggatcgaa atagagcggg
    12421 ttaggcccca cccagagcag cgtcgccgtc ttcagcacga ccgaaacgcc gagcgtgatc
    12481 atgacgatgg tcatgacgct gccgtttcgc acgcgcgaca ccgcgccgaa gtcgatcagc
    12541 gcgccaagcg cgccgaccgc ggccaccgtc gctatgatgg ccagccatag cggccagtgc
    12601 cactcgccga tggcgatgca ggccaggacc ccgcccagca tgacgaattc gccttgcgcg
    12661 aagttgatga cgtcggtggc gttgaagatc aggttgaaac ccagacccac caacgcatag
    12721 atggcgccga ccgtcagtcc actaatggcg aattgcacca gttgggcgga atccatgcag
    12781 cggctccccg atcagaaacc gtgctgtacg ccgaccatac ggccgtcgcg cacctgcacg
    12841 atccagaccg agtctacccc cagcccttca tgattgtcgg cggtataggt gtaggtgccg
    12901 gctgccagtt cgtatttcga gatctgctcg aagccttccc gcatggcgtt gcggtcgccc
    12961 ttggcgtttt ctatcgcctt gacgaccaga tgcagcgcat cccagccgat cgtgtcgtag
    13021 atcgtcagct ggtagtcgtc gccgttcttg tccttgtaga tcttggcgag gtcggtctgt
    13081 ccggcagtca cctggtcggg ggccgtcagt gtcaccatgt agacgccttc agcggcggcg
    13141 ccggcgccct tgatgatggc ggcggcggcc gtgccctgcg aaccgatgat cggcgccgta
    13201 ttgccgagct gcgatccgtt gcgcagcgcg atggacatgg tggagcctga accccacagg
    13261 atgatcgccg ccggcttggt ggccagcagc ttcgtccatt cggcggtggt gtcggtcgcg
    13321 ccgtaattca ccttctccgc gccggtgatg ggcaggccca tcgccttcgc ctccacctga
    13381 agtgcggcat aggcgtcgct gccataggcg tcgctggtgt agaggatgcc gacatttgcg
    13441 gccttgagct tgtcacgggc gaaagtcagc gcggccttgg tattgtcggc gtcgctggtc
    13501 tggcagcgga agatataagg gttgccgagc ttggtgatcg aaatcgtgtt gttgaaggaa
    13561 catgccggta tcttggcctg ctgcaaggcc gcggatgcag caagcatgtt gctgccctgg
    13621 ttcggcccga tgatggcgta gacgccttcc tcggccagtc ggcggaaatt ctgcacggcg
    13681 atgtcgggct tgctctggtc gtcgcggatc accagttcga tctggtggcc attgacgccg
    13741 cccttggcat tgatcgcgtc cgccgcgatc ttgacgctgt ccaccaccgg gatgccgagc
    13801 gaactggcgc cgccgctcaa cggctccgaa acgccgacct tgtaagtttc ctgcgcatgc
    13861 aggggcgaac cgaggcaaag caggctgccc gcaacgacga gagacctgag aactgcacta
    13921 ccgttcatca cttcctccac tgattttccg gctgtttgcg aacgccggct ttctgttccc
    13981 aatcgaggct gcgccgatca tctccctacc ggcgcagcaa ttatctttac gtcaggcgat
    14041 cgacttcgcg ctcctgaccg tctcgcccag cacctgcgag aacaggctgg tgctcgaaaa
    14101 caccgcgatc gggatgccgc gcttgcgaac atcggcgact tcggcagaac tgcgcacgaa
    14161 ggtagaggcg accgagccgg cggcaacggt atccttgacc accttgtcga tcgccgccac
    14221 cacggttgga tggcccggat tgccgggatg gccaagcgag atcgaaaggt cggcaggccc
    14281 gatgaagatg ccgtcgatat tgtcgagcgc ggcgatggcc gacacatgat cgagggcctt
    14341 ggcatcttcg atctgcacca gcacgacggt gttttcatag gctttgttca gatgctcggt
    14401 aagcgtggtg aagccatgcc gcccggcccg cgccgtcgtc gccagccccc tcacgccatg
    14461 cggcggataa tgcgcggcag ccgcgatgga tgcggcctcc tcgggcacac acacatgggg
    14521 caccaatatg ccggtggcgc cagcgtccat gatgcgcagg atttcgccct gcgtcatgcc
    14581 gaccgtgcgg accagcgccg gcaccccggc ggcttccgcc gcgcgcagct ggttctccag
    14641 cgtttcgggc gtcgccgacc catgctcggt gtcgaggatg aggaaatcga agcctgcata
    14701 ggccaccatt tcggttatcg acggcgacgg caccatgacg aaacagccga cgagactttc
    14761 tccggcgcgc aactttttct tcaagacgga caaactggac ctcccatgca attatcgtcg
    14821 aacagacacg acctcaggtt tgcgtcccgg ccatccattc ccgtacaggc agatgaaatg
    14881 tgcgcatact gcgatctgtg atcacattta tattgaatta tgacgactgg gttgtctaga
    14941 tgcattcggt tgtgaaaaaa gaaaacgcgc cacacttttg gggagaaacg cgatgaagtt
    15001 gttcgatctg agcgagcggg tggcgattgt caccggcggc aatggcggga tcggtctcgg
    15061 catggcgaaa ggcctttgcc aggcgggcgc gcaaacggtg cttgtcgggc gcaatcgcga
    15121 caagggcgaa gcggcggccg gcggtctcgt cggcgttggc ctgaaggcaa gcttcctcga
    15181 agccgacgtt accgacgagg caagctgccg cgaactggtg aaggccgtga ccgagaggca
    15241 tggcggcgtc gacgtgctga tcaacaatgc cggcatcgcc attcgcaagt cgccgcagga
    15301 ctacacgctt gccgaatggc acgcggtgct cgacaccaac ctgacaagcg ctttcctgac
    15361 atcgcaggcc gtctatgaca gcatgaaggc gcgtggcggc ggcaagatca tcaacatcgc
    15421 ctcggtgctg gccctcttcg gcgcgccgtt cgccatcgcc tactcggcca gcaagggcgg
    15481 gctggtgcag ttctccaagg cgctcgccac cgcctgggcg cccgacaaca tccaggtcaa
    15541 cgccatcctg cctggctgga tcgacaccga actgacggtc gaggcgcgcg cgcaggtggc
    15601 cggccttgcg gaaaaggtga tcgcccgcgt gccggccaag cgctggggca aggtcgagga
    15661 ttttgccggg gccgccgtct tcctcgccag ctcggcctcg gatttcgtca ccggttcgag
    15721 cctgaccgtc gatggcggat tttccgcgca gggctgacgc ggcagcgtcc ggccaccgcg
    15781 tcaggctgaa atgcccgtca gcgaagcccg gtcatcttgt tccaggtctt gacgaacccc
    15841 gcccgcatcg gcgtgtagat cgaccagtcg gtcggcaaga gcaggttcaa ttcgctgatc
    15901 ggcacgccgc cgggaggcgg cgcgacatcg gcgcgcggcg aatgcaggaa cacgtaatcg
    15961 gcctggatgc gctggccgag cggcgagagg aaccagtcga ggaacaggcg cgccgcttcg
    16021 ggatgcgagg cttccttgat cacgccccac acttccgggc cggccggcag gccgcttggc
    16081 ggcaacacca tttcaattgg agcgcctttc tgcttgtaga gcaggtagcc ggaatattgc
    16141 gcggtgacgg ccacctcgtc gtcgccgttg acgatgcggt cgaactgctg gacgtaggaa
    16201 tcgaaagcgg tcggcttcag ttcggcgatc ttcggccaga aaccgtcgcc cagtttcttc
    16261 cccagcagat cccacgccag cagctgcaag ccggaattgc tcgatttcac gctgagcttg
    16321 cccgcccagc gggggttggt gaggtcggcc caatccttgg gcgcttcatc cttcgccagt
    16381 atcttgggat tataggcgat gcccgccatg atgaccgcgc cccaggtcca cagcccttcg
    16441 ggtttgcttt tatattcgct cttgtaggcg ctgtcctgcg gcgagacata ggccccgtag
    16501 ccgccgtggt tctggaagtc gagcaccagt ccggtgtcgg aaagatgcag cacgtcggca
    16561 aggaaactgc ccgactgccg ctcgcgcatc aggcgggcat agagcgcgcc gctttgcagg
    16621 cgcacatagc tggtcttgat cttggggaac agtcggtgga attcctcaag cagtttcacc
    16681 gtcccaggtt cgggtgcggg agaatagaga acgacctcgc cttcctcctg cgccttggcg
    16741 atatccgcgc aggtggcgaa accctgcacc tgctggggag aggggcaggc cttggtttgc
    16801 ctgtcgtcgg cgcgggcggc cgtgcccagc agggaaaagg ccgcgaaagc cagtatcagt
    16861 ctgcgcatgt tccattcctc cattgccttc aattcttcag cagcacgcaa tgctcgggct
    16921 tcaggcaggc cagcaccgcc gatcccggtt caaacatgtt ggtggggctg ccgcgcacca
    16981 tcaggtcgcc gatcggcgtt gcgacgcggt actggatgaa atcgccgtgg aacatgcgcc
    17041 gcacgatggt tgcggcaagc acgttgagcc gtgcatcctg cggcttttcg cgctccaggg
    17101 cgacataggc cgggcgaatg gcaatctcgt tttccgtgcc gtccggctgg aacggcgaca
    17161 gcgcctgcac cgtcacccca ttggcctcga aggcgatgct cccgaccgca ttcggacctt
    17221 tcaccttgcc gcgaacgata ttggccgacc cgacgaaatc cgcgacgaaa tggttggccg
    17281 gcctttcata gatggtttcg ggcgtgccta tctgctcgat gcggccggca ttcatcacca
    17341 ccaccttgtc ggagatcgca aaggcttcct cctggtcatg cgtgacatag agcgaggtaa
    17401 tcttcaggcg gtcgcgcaga ttgcccagtt cctcgcgcat ctcgttgcgc agcttggcgt
    17461 cgagattgga cagcggctcg tcgaacaaaa cgactttcgg cgaaaacgcg atggcgcggg
    17521 ccagcgcgac gcgctgctgc tggccgcccg aaagccggga ggcggcccgg tcggcatagg
    17581 gcgtcatctg caccaggtcg agcgccttgc gcacggcatc ggcaatggtc gccttgtcct
    17641 gccggcgcac gttcagccca taggccacat tgtcgaacac gctcatatgc ggccagatgg
    17701 cgtaggactg gaagaccatc gagatgccgc gcttttcggt cggcacgtcg acgcgctgcg
    17761 cctccgaaaa gaacggcttg ccgtcgacaa ttatctcgcc gccggagggc cgctccaggc
    17821 cggcgatgga gcgcagcgtc gtcgtcttgc cgcagccgga tggcccaagc agggtcaggt
    17881 gctcgccccg cccgaccgag aaggaaacgc cgcgcaccgc cgtcaccgcg ccatagcgga
    17941 tgaccagatc ggtgacgtcg atctgcgcgc cgagttcgcc ggcggcgtgg gagccttgcg
    18001 agcctgatgg ggtcaaagcg ggcctccctt gtcgatgatc tgcccgctct ggcggcggct
    18061 gatgtagaac agcaccagca cggcgatcag caccgtcgcg gtctgtatga cggccatggc
    18121 ggcggtgagt tcggtgccgg agctggacca ggaactgacg atggccggcg cgatgacctt
    18181 ggaattcggc cccatcaggt agatcgagac gccaagctcg cgcacgctgg cgatgaacag
    18241 cagcacccag gccgagatca tgcccggccg catcagcggc agggtgacgg tgcgcaattg
    18301 ctggctccac gaagcgccgc agacgcgggc gcattcctcc aggctgcggt cgatttgcag
    18361 gaccacgccg gcaagcgtgc gcaccccaag cggcagcagc accgtgaaat aggcgatcgc
    18421 cagcagccac agcgtgccgt agatcggaat cggcatgttg agccacgccc acagcagcgc
    18481 caggccgaag accatgcgcg gcaccgcctg cgggaacatg acgacatatt ccatcacgcc
    18541 ccggcccggc gccctcgacc gatagatgat ccagacgagc agcatcatca cgaagacgcc
    18601 gacggtcgcc acgccaaagc cgagcatcag gctgttgccg atcgcgctgc ggatggaatc
    18661 gaggctgaac gcggtggcgt aattggccag cgtgaattcg ctttgcgaca tcaaaacggt
    18721 tgcgaaacgc tggaacgagg tcagcacgag ggccgccagc ggcagcacga cggcaatcac
    18781 gatatagagc cagcaaaagc caagcagcag ccagcgggaa cgcccggcat cgatggcgcg
    18841 cggctggaac gccttgcccg aaatggtcgc gtagctgccg cgtcgcacaa cccagcgata
    18901 gagcgcgagc atggcgaaca tgacggcgaa cagcgaaatg ccaagcgcgg cggcccgccc
    18961 gtaatcaggc gggtaggaca aggtgagttc ccatatggcg gtggtaatca cgtagaaacg
    19021 gcccggaatg cccagcacca gggccgccgc gaaggagccg agcatttcgg cgaacacgaa
    19081 gatgaaggcg cccatcacgg ccggaaaggt caaaggcagg gttacgcgca tcatggtccg
    19141 gaacttgccc gcgcccagca cgcgcgaact ttcctccagc gacgggtcca tggacttcat
    19201 ggcggcggcg atcatgacga aggcgacggt gccctcgaac agcgccatca cccaggcgac
    19261 gccttcgggc gtataaatat cgacgagcgg tcccgaaccg ccgagataca tccacgcctg
    19321 gttgatgaac ccgctcttgg gcgcggccag gatcgcccag gcgagagcgc cgaccagcgg
    19381 cgtgacatag aacggaattt cgatcagccg ttccagaaac cccctgcccg gaatgcgggt
    19441 tcgggtcagt atccatgcca gcacgaaacc gacggtgacg gaaatgatgg tggccacgaa
    19501 ggcgacacgc agcgtgttgc cgatcaccgt ccagtccagc gccgattcca gatagttcgc
    19561 gaggccgatc tcttcgggcg ggaagatttg cggatcgccg atattcagcg attccgcaac
    19621 gaggaagacg accggataga gaaccaggaa gccgcagacg acaaggatga gcagcgtcac
    19681 gaaggcctgg ccgcgaaggc cgggaaccgg caggcgccag cgcggcgcgc gggctcgctt
    19741 gtccatcccg gcctcgcttt ccgggctcat ttcatgcccg tcatcgaccg catctggcgc
    19801 aggaactcgg tgcggctctt ggcgaaggcc gcccagtcct gcggccacag aaggtgcatc
    19861 gccgtgatcg gcagcgcgcc ttccggcggc gcgacgtctt cgcgcggcga atggaaatac
    19921 cagtaatcca ccgacgcctt ctggccgcgc ggcgaaagat accagtcgag gaacagccgg
    19981 gcggcgttcg ggtgcggcgc ggtgttgacg acgccaacca cttcggggct ggccggcagc
    20041 ccgctggtcg gcgcgatgaa ctcgatgggc gcgcccttgt gcttgtattc gagataggcg
    20101 gcatattggg cggtcagccc gatcttgtcg tcgccattca ccaggcggtc gaactgctgc
    20161 accatggaat cgaaggcgcg cggcttctgc gcggccatct tgtcccagta ggtgccgtcc
    20221 atctggctgc gcagttcgta ccacgtcaca tgctgcatgc cggaattggc ggatttcatg
    20281 gagacggcgc cgtcccattt cgggtccagc gcatccggcc agtccttcgg cgcctgatcg
    20341 ggcggcacga tcttggggtt atacgccatg ccggcgggca tcaggacggc ccatgtccag
    20401 tagccttcgg gcgcgctgcg gtattcgggc ttgtaggccg gcagttcggg cgaaacgtag
    20461 ggcgcgtagc cgcccttgga ctggaaatcc agcaccagcc ccatgtcgcc gatctggaaa
    20521 atgtcggcca ggtggctgcc gccctgccgc tcgctcatca ggcgcgcata gagcgcgcct
    20581 gcctgaagac ggatgtagct ggtcttgatc ttcgggaaca gtttctggaa ttcgcccagc
    20641 accttgacgg tgccgtgctc gatatcggtg gcgtagaaga caagctcgcc ttcctgttcc
    20701 gccttggcga catcggcgca ggtcttgaag ccctgcacct cctgcggtga tgcgcacaca
    20761 accggctcat ccgccgctcg ggcaagctgt atgccacccg ccagtatcgt tgccgcgaac
    20821 agaacttgga atttcatcga aacctccctg gttgccgcgg ccgctcatcg gcagcgtttg
    20881 attctggctc ctgcgccgat ctaagcgtcc ttcttcccgt aggacccggc gcgcgccttg
    20941 tagatcgggt cgtcgtggca ggccccggac gccagcatgt ggtagacgcc gaccatgccg
    21001 tgcgcctgcg gcggttcggg gtccatcgcc accttggaca ggaacagcgc catggaactg
    21061 cgctcggcca tcgcctgcgc gaaggccatc gcttccggca tcagccggtc gagcggatga
    21121 accgcattgg cgaggccgcg ctggtgggcc tcctccgccc cgatgcgccg cccggtcagc
    21181 gcccattcct tggcgacgcc cagcccgacg atgggcggca ggcgttgcag accgcccgaa
    21241 ccgggtatgg tgccaaggac gatttccggc agcgagaaca agacattatc ggcgacgatg
    21301 cggatatcgc aggccagcgc catctcgaag ccgccgccgg cgacatggcc gtgcagcgcg
    21361 ccgatcaccg gcttggagca ggccgcgatg cggttcttgg tggaaaaatc cagcttgaga
    21421 tagttgcggg ccgccacccc ctggaactgg ccgacctcgc gcaggtccga accggaacag
    21481 aatgccctgc ccgcgccggt gatgacgacc accttgacct gttcgtcgcc ctcggcccag
    21541 tccatcgccg cctgcatttc gcggtgggtg gcggaattgc aggcgttcag cacctctggc
    21601 cggttgagcg tcaccaccgc gacggccccg tccacctgcg tgcgcagata ctggaactcg
    21661 ggcgcgctca tttgggccgc ttccggatca gggttatgtg ctcgccgttc tggaccacct
    21721 cgcccttctg gttcatcagt tcgaccagtt cggtgatgat gcccatgttg ggcttgctgc
    21781 cgctttcgcg cttttgcgtc agttgccagc gcacgcgcac cgtatcgccg atcatgatcg
    21841 gcttcttgaa cgtccatttg tcccagccga gcgaagccac gcccgtgcct tgcgtatagt
    21901 tggtgcgcca catcaggccc tgcgcgatgg aaaggccgag cagcccatgg gcgatgcgcg
    21961 tgccgaactc ggtcttcttg cagaattcct catcggtatg gacctggctg taatcgccgg
    22021 tcaggcccga aaacgcgacc agatcggtct cggtgatcgt gcggccgggc gaaaaccgtt
    22081 ccttgcctgt ttcgaaatcc tcgaaccaca ggccgagcac gacaggctcg ggcatcgtta
    22141 tcaaagtcat tcctcgtttc ctccaaaggc atgttcgaga ttcatgatgc cgtcagcgaa
    22201 tatctccgcc ggcatctgct tgaggtcgtc cgagacgcga aggtcgaagc ctatctgcgc
    22261 gcggatatcg cgctccaggt cgatgccggg cgcgatctcg acaagttctg gtccggcggc
    22321 ggtcagcttc atcacgccgc gttcggtcac gtagagaacc tcttgtccct tctcgatggc
    22381 gcgcgcggcg ttgaacgtaa cctgctcgac cttggcgacg aacttgcggt gccgtccctc
    22441 ctgcacgatc tgcgtcttgc cgccggcaac ctccaccttc aggccgccgc tcttcagcga
    22501 gccgcaaaac acgatgcgtt tggtgcggtc cgtgatgttg ttgaagccgc ccggaccatt
    22561 gatctcctcg ccgaagcggc tgacattgag gtcgcccgcg ccgtctacct cgccgatgcc
    22621 gaggaaggtt atgtccagcc cgccgccatc gtagaaattg aactggaata ccgaatccat
    22681 gatcgcttcg gcgttgagcg ccggcacgaa ggacccgccg agcgcggtcg ccatcatgcc
    22741 gccgaacaca ccatgctcgt tggtgaaggt gacccggtcg agaatgcctt cctccagcgc
    22801 caccagcggc agccgggtag cgacgcccgc gccgagattg accacgtcgc cttcccgcag
    22861 ttccagcgcc gcgcgccgca atatcgtccg ttcagaaccc ttcttgacgg ttggcaggcc
    22921 cttcagggga tgtttcatcg cgccggccag aaacgggttc cagccattca acgggtcggc
    22981 catcgccggc gaaagctggg tctggtgcgg gttgacgacg acgacatcga ccagcggacc
    23041 gggtatgcgc accagccgcg gatcgaggct gccggcgcgc gccagccgct tcacctgcac
    23101 aatgacgcgc ccgcccgacg ccttgcaggc catggcgatt tcgaccgcgc ccagcgtgtt
    23161 ggtctcttcc tcgaagctga tatagccgtt ctcgtcggcg gtggtgccgc gtatgatggc
    23221 cacatcgatg ggaaaggacg ggtagaagag atattccttg ccatcgacct tctcgacccg
    23281 gcagggcggg cgtgtcgact gcgcgttcag cgccccgcct tcacggcgcg gatcgagaaa
    23341 ggtgccgatg ccaaccggcg tgaaaagacc gggcttgccg gagccgatgg cagtcaacag
    23401 ctccaccatc gaacccatgc tgatcatgta ggcttccacc tcgccgccgc gcagaagatc
    23461 gagcaggcgc ggatgcctgg ccctggcgaa cgaggcgccg acgaggcgct tcagcatgcc
    23521 gggatgggca aagctgttca tgccgccctc gcctggccgg tcgccgggca gcatcggcgt
    23581 gatgaggttc agcgcgcgtg gatggctctc ggccaggaaa cgctcttcca gcgccttgag
    23641 caccaggtct ggctcaagca ccggtcccgt gccgctgacg cagaccgtgg ccccgtccgg
    23701 tatcagggat accgcctgcg cggcgtcgag aactttaggc attcttccca tctgtcgtcg
    23761 gttccccaag attctagtct attgcgccgg ccggctctcg ctccacagcc gccggacgtt
    23821 catcggtccc ggcgcgaagc acatggcagg cattttccgc aaagtttccg ctacttctat
    23881 ccggcacggc atcgcgtcga gcaggtcgcg gcgcggatcg acgccgggcg cgatctcggt
    23941 cagcgtaagg ccgttctccc tcagttcgaa aaccgccttt tcggtcacgt aagtgatgtg
    24001 ctggccgcgc cgataagcct cttgcccgtt gaaggatatg ctctgcacct gatcggtgaa
    24061 tttcagtccg gccctgtcgc cgacgacgaa gcagaagacg atcttcctta tgccttgcgt
    24121 catttcgacg aagccgccgg agccgggtat gcggtcgccg aacctgccga catttacgtt
    24181 gccgcttgca tcgacctcgc cgacgccgag gaacgcgcag tcgatgccgc caccggcaaa
    24241 gaagcgtatc tggtcggcgg catcgatgat ggcggtcgga ttatgcgcga cgggaaaggt
    24301 cttgccgccg gccgcatagc cgttgacgcc gccgaacacg ccgtgctcca cggtgaaggt
    24361 gacgtcgtcc agcacgcctt cctcgatggc gatggccggc aggaagccag gcacgccgta
    24421 gccgatggcc aaaagattgt cggggcgcac ctccaggaac gcgcggcgca tcaccacctt
    24481 ggcggaatcg agcggccgca ttggcacgct tgcaatgtcg agctgctccg ccccgacaag
    24541 cgtagggtcg aagacgacat tggtgatctg cttctgcgcc gggttgacga cgacggcatc
    24601 gaccagcgcg cccggcacgc gcaccatatg cgggttgagc gacccggcgg caaccacccg
    24661 cttcacctgc gcgacgacct tgccgccatt cgccttggca gcctgcgcca gcaccagcgc
    24721 gccgataatg gcaggttcct cctcgaaggt cagattgcca agttcgtcgg cggtggtggc
    24781 gcggatgaag gcggtgtcca cggggatcgc ccggtaatag agaaaatcct cgccgtcgat
    24841 ttcgatgcgc tggacgagct tttcgcgcga tatcgaattg agggcgccgc cgccatgaat
    24901 cgggtcctcg aacgtgccga gaccaacgcg ggtgatcaag ccgggccggt tggcggatgc
    24961 cgcctccagc agcccataca tgaccccgat ggaaaaatta tacgcctcca tctcgttggc
    25021 gttgatgcgc cggatgagtt ccggcacgcc gaccggccat agcgaaccgc cgatcatgcg
    25081 cctgaccagg cccgccgtgc cgaaaagact ggtgccgcgt ccctcgatct cgccggtgcg
    25141 gatcgggtgg aattccgtaa ggccggcggg cgatccggtt tccttgaact gccgcaccag
    25201 cgcctcgatg agcaggtccg gctcctgcac gccgcctgcg ccgccaaccg ccaggcagct
    25261 gcccgactgg acgaaggcga cggcttcctc ggcggacatg atcctggatt tgcgtttttc
    25321 catcaggcct gccccttgtc ttccagaaaa ctgccggcat tggcccggta atccttggcc
    25381 gactggcaga cgctgctgac cagtccctgc accatcggcg cgatcgcccc atcggccggc
    25441 agcctgatgc aggtcttggc atagcggatc gcctcagccg ggcgctgcga cagcgcgcgc
    25501 gcccattcga gcgcttccgg caaggcccgc ccgtcctcga ccacgcggtt ggccaagccg
    25561 atttccacgg cgcgggggcc ggcgacccgc gtgccctcca tcaccagttc cagcgcccgc
    25621 gatggcccga caagcgcgtg gagacgctgc atgccgcccg aaccgggcac gctgcccagc
    25681 gtcatttccg gaaagccgaa agcagccgtt ttctccacca gccgcatgtc gcagcccagc
    25741 gccagctccg cgccgccgcc aagcgcaaag ccgtgaacgg cggcgatgct cagcagcggc
    25801 gaccggttta tgcgttcgcc cacgatccag gtgcgctcga tgaagcgccg cagcatctcg
    25861 ccggcaagcg tgcgcatgtg cttgaggtcg gcaccagcgc agaagctgcg cccctcgccc
    25921 gcgatcacca gcacggacgc ctgctcctgc gcctcgattt cgtcgagggc ggcgataatg
    25981 gcgtcgcaca tggcgcggtc gatggcattg agcacgtcgg gccgcgccag cgtgaggacc
    26041 gcgacccggc cctccaccgc cagcttcacc cggtccgcca tggtcacttc gcgcctccga
    26101 accggccagg ttcggccttg gcggcgccgt tgccgacatg ccgcaccatg atcgggcagg
    26161 accatttggc gtcgtcgcgg aaatcggcat cctcgcggta atggctgccg cgcgtctcgc
    26221 gccgctcgat cgccgccgcg cacatggcgc gcccgacatc gatcatgttg acggcctcgg
    26281 tgaacgccac cacgtccttg acggtctcga taccgaactg tcccgcttcg agcgccgccg
    26341 ccagttccgc aagctccgcc tcggcggtct tcagcccttt ttcgttgcgg accaccaagg
    26401 cgtggtcggt catggtctgc ttgatgcgtt gatgcgcctg cctgatcttc aacgtaccgt
    26461 cgccgccggt cttggcctgc aagtccgcga ccgccgcttc ggcaagccgc tcgacattgg
    26521 ccgctgcgcg cgcgcgcccg atctccgccg cccggcggcc ggcgcgcttg ccgaacacct
    26581 ggcagttcag cagcatgttg ccgccaaggc gatccgctcc atagggaccg gcggcattct
    26641 cgcccgccga cagcaggccc tcgaccgagg tctggccatg ctcgtctatg acgaggccgc
    26701 cattgatggc gtggccgaac aggccgatgc gcagcggcgc ggcgtcgatg tccatgtgct
    26761 tctggcgcag ccagcctttc gaaacgttcc acaattgcgt gaaacccttc tgggtcagct
    26821 tgtccgggtc ggtggtgcgc aggtcgagga agaacgagcc tgtctcgtcg gcccgcccat
    26881 cccgcatcgc attcttggtg gcgatttcaa gccatctgga agcatcggac gcgctgaagg
    26941 gatagtgttc gaccttcttc agcatcgcgt cggcggtgct cgtgccggcc ggcagcgcgc
    27001 cgtccagaac cggcctgccc tcgcggtcga cgaagggcgg aacgccgccc cagaaccatg
    27061 tatagacgat gttcagcgcc ggcttcaccg tgccgaaccc ggcctgcatg aactccatgt
    27121 tggcgagcgt cgcgccggcg cggtagccca gcgcatagcc gtcgccggta atgtctggcg
    27181 gcatcaggct gagcttgaac agctgccccg ccccgccggt ggtcagcacc gtcgcgcccg
    27241 ccttgacgcg gatgagctgg ccctgccggg tcagcgccac caggccgtga acgcccttct
    27301 cgtcctgcaa gagcgacagc acggtggtgc cttccagcga atggacgccg aggcgcgccg
    27361 tctcgccctt cagggcgacg gtgatcggcc ggccatgatg gtagatcttg cggttgcgcg
    27421 gacgcgaggc aaaatcgccc tgcgcgacga gcggcttgcc cgtcgcctcc tcgcggatga
    27481 aatcgatgtt ccagcggtcg aggtcttcgg cggcttccag cgcctcatcc accagaatgc
    27541 gcaccagcgc cgggtcggca cagccctgcg ccgccgtcat gatgtcgtta tagtggacat
    27601 ccggattgtc gagcggatcg cccgcgccgt ccggcaccgc gaaacccgcc gtcgaggcga
    27661 ggccgaccgc cgtagcgccc gaaaccgcga tgcgcccctt ggtgatcagg atcgtccgcg
    27721 cgccggccgc cgccgcctca taggccacgc gcagcccggc tcctccggcc ccgacgacga
    27781 cgacatcggc cgtcagttcc tccatcgcgc cgaaattcat gccaggcctc ccggcgccag
    27841 cgtgtcgcca aggcccaccc gctggacccg gctggcgatc atgccgcgct ggatgtcgga
    27901 cgttccgtcg aggatgcgat aggagcggca atcgcgaaac aggcgctcta cgccgaagtc
    27961 ctgcgatacg cccatggcgc cgtgaacctg caccgccgca tcggcagcgc ggccggccgc
    28021 ctcgctgcaa aacagcttgg cgtaagccgc ttcctgcgcg gcgtcgtggc cttcgtcaaa
    28081 gagcttcgcc gcctggtaga gcagcagcct tgccgtgtag aggtcggccg acatgtcggc
    28141 gatcttgtgc ttgatggcct ggaactcgcc gatcttcttg ccaaactgga cgcggttttc
    28201 ggcatggacg aggctgagtt cgagcgagcg tccgatgacg cccaccgcgc gggcggcggc
    28261 ccacagccgg gcgcggccaa ggatgaattt cagggcgctg aagccgaagc ccacctcgcc
    28321 cagcatttca tcgtcgctgg cctcgacctc gtcgaaattc acctccgcgc tgcccgatcc
    28381 acggccgccc agctgccatt ccggcttgcc gacattgatg cccttgcgct tgtcgatgag
    28441 gaagacggtg atgccgccct tcgcgccctt ttccggatcg gtaaccgccg agacgaggaa
    28501 aacgccggcc ttggtggcgc ggctgatcca cagcttcgtg ccgctgatcg accagcggcc
    28561 gttcttgcgc acggcgcggg tcttgatgcc ggctgcatcc gagccggcgc cggcctcgct
    28621 caggcagaac gccgcgtagc gcgaaccgct tgcgaggtcc ggcaggaagc gctctttctg
    28681 atcctggcgg ccaacgagat tgatggcggc ggcaggcgcg ctgggcgcga tgaagaacgt
    28741 caccccgccc ggcccgtagc tcatcgtttc cagcgcgacc gaggaagcga gctcactttc
    28801 gcccagcccg ccataggcct ccggaataga aaacccgaac aggccaagct cgcgggcctt
    28861 atcgagaatg tgctcgggaa tctcgtttgt ccggtctatc tcggcggcat gcggctcgac
    28921 gatgtcatcg acgaaggctc tcacggcgtc acgaagctga cgcacgtctt ctggcaacct
    28981 taatggcact ctcaatcctc ccgatccgca ccgaagttcg cgataatctg cgatctgtga
    29041 tcacatattt taatcagcgt ggtacagatt gcaagctggt gctcacattt gttttgcgga
    29101 aattgc
//
LOCUS       DASYCS010000181         1684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4682597,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000181 DASYCS010000000
VERSION     DASYCS010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4682597"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="VGM46_05925"
     CDS             <1..781
                     /locus_tag="VGM46_05925"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4192164.1"
                     /translation="GSYLAGAIWPPIFERVIGTYGWREAMLWYGAAEIIVIVPLALIY
                     FSHPPEVIHPPVATNGAAKPRVLGLSSNTMFVLMCVAPFLCCIPMSIPQGHLVAFCSD
                     LGISRTMSALMLSTLLGTAFLSRQIWGVISDKIGGLTTILIGSAWQALAMTAFLFTQN
                     EAGLFTVSAAFGLGFAGIIPAYVLAARELFPPSEASWRIPTLLLSTGVGMAFGGWLAG
                     LLYDYYGYYAPAFAVGIGSNLLNIVLIGWLVLRWRNRAAYA"
     gene            complement(785..>1684)
                     /locus_tag="VGM46_05930"
     CDS             complement(785..>1684)
                     /locus_tag="VGM46_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEY4192165.1"
                     /translation="LSTGSLSRFTPLQSEAVMTHAPGAESMLWLPARAVLGDPVALVV
                     VIGVSLILLVASIVIFAGNFGEHATAASGVSSTVVRQRPARGFRRRSPGSVLRHKEWT
                     LLLRDPWLISQTLMQILYLLPPALMLWISFREGGGALIVLVPVVVMAAGQLSGGLAWL
                     SISGEDAPDLVATAPVMARRITIAKVEAVLGAVAFIFAPLVIALAFASLFAALICGLG
                     VLIAGAAATYIQLCFRVQARRSQFRRRQTSSRIATFAEAFSSIGWAATSALAASGSFL
                     TLFPALMAFAILAGVRVIAPKSA"
BASE COUNT          308 a          544 c          544 g          288 t
ORIGIN      
        1 cggcagctat ctcgccggcg cgatctggcc gccgattttc gagcgcgtta tcggtaccta
       61 cggctggcgc gaggcgatgc tgtggtacgg agcggccgaa atcatcgtca tcgtgccgct
      121 ggccttgatt tatttcagcc atccgcccga agtcattcac ccacccgtcg cgaccaatgg
      181 tgccgcgaag ccgcgtgtgc tcgggctgtc gtcgaacacc atgttcgtgc tgatgtgcgt
      241 ggcgccgttc ctgtgctgca ttccgatgtc gataccgcag ggccatctcg tcgcgttctg
      301 tagtgacctt ggcatcagcc ggacgatgag cgcattgatg ctgtcgacac tgctcggcac
      361 ggcgttcctg agccgccaga tttggggcgt gatctccgac aagatcggcg ggctgacgac
      421 catcctgatc ggttcggcct ggcaggcgct tgccatgacg gcgttcctgt tcacgcagaa
      481 cgaggctggc ctcttcaccg tatccgcggc tttcggtctc ggcttcgcag gcatcatccc
      541 cgcctacgtg ctggcggcgc gtgaattgtt cccgccatcc gaagcatcgt ggcgtattcc
      601 gacgctgctg ctgtccaccg gcgtgggtat ggccttcggc ggctggctcg ccggattgct
      661 gtacgattat tacggctatt acgcccccgc ctttgcagtc ggcatcggct caaacctgct
      721 caacatcgtg ctgatcggct ggctggtgtt gcgctggcgg aatcgcgcgg cctacgcttg
      781 agcatcaagc gcttttcggc gcgatcaccc gcacaccggc aagaatggcg aacgccataa
      841 gggccggaaa tagcgtgaga aagctgccgc tggcggcaag cgccgacgta gctgcccagc
      901 caatcgaaga aaaggcttcg gcaaacgtcg cgatgcgcga cgaggtctga cggcggcgga
      961 attggctgcg ccgcgcctgc acccggaaac acaattggat gtaggtcgcc gccgcccccg
     1021 cgatcagtac gccgaggcca cagatcaacg ctgcaaagag cgacgcgaag gccagcgcaa
     1081 tcaccagcgg cgcgaagatg aaggcaacgg cgccgagcac ggcttcgact ttggcaatgg
     1141 tgatgcgacg cgccatcacc ggcgcagtgg cgacaaggtc cggcgcgtct tcgccggaga
     1201 tcgagagcca ggcaagaccg ccggaaagct gcccggccgc catgacgacg accggcacca
     1261 gcacgatcag cgcgccgccg ccctcccgga agctgatcca cagcatcagc gcagggggca
     1321 gcagatagag tatctgcatc agggtctgcg atatcagcca cggatcgcgc agcagcagcg
     1381 tccattcttt gtggcgcagg acgctgccgg gcgagcggcg gcgaaagccg cgcgccggac
     1441 gctggcggac cacggtactg gaaacgcccg atgccgccgt ggcgtgttcg ccgaagttgc
     1501 cggcaaaaat cacgatcgac gcgacgagca ggatcaggct gacgccaatg acaacgacaa
     1561 gcgcaaccgg gtcgcccagc accgcgcgcg ccgggagcca cagcatgctt tctgctccgg
     1621 gcgcgtgcgt catgaccgct tccgactgca acggcgtgaa gcgcgacagg ctgccggtcg
     1681 agag
//
LOCUS       DASYCS010000182         2622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3087683,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000182 DASYCS010000000
VERSION     DASYCS010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3087683"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="VGM46_05935"
     CDS             complement(<1..514)
                     /locus_tag="VGM46_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY4192166.1"
                     /translation="MSILRGKRVLVTGGAGFVGSHIVDQLVAADCARIVVVDNMVRGR
                     IANLVSALPSGRVEIVNGDIRDQRLMQTLVAGCDTVFHQAALRITHCAAEPDEAMQVM
                     VDATYRLLRQCVEAKVRKVVMASSASIYGMAEDFPTTESHHPYGNRTLYGAAKSFGEG
                     LLRSFNDMYGL"
     gene            complement(545..1660)
                     /locus_tag="VGM46_05940"
     CDS             complement(545..1660)
                     /locus_tag="VGM46_05940"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEY4192167.1"
                     /translation="MIPFLDLRAQYQSIGPQLEQAVVFAMRECNFVLGDRVEQFERDF
                     AAYCGTGHAVALNSGTSALHLALLAAGIGPGDEVITVPMTFVATTAAVVYAGATPVFV
                     DVDPATWTMDADKIAEKVTSRTKAIIPVHLHGRLADMTSICGVARRFGLTVIEDAAQA
                     HGAERDGVRAGNFGDIGCFSFYPGKNLGACGEGGAITTNRADLAAAVTSLRDWGQEGK
                     YNHVRHGFNFRMDAVQGAVLGVKLPMLDGWNAARRRIANLYHEGLFGDLSLPAGPFGL
                     DHVCHVYAVRVSDRDHVRASLHDAGVASNIHYPVPVHLQPAYANLGYGAGDFPVSEAL
                     GRETLSLPMFPELADASVARIVEALNEATAQPLARTA"
     gene            complement(1657..>2622)
                     /locus_tag="VGM46_05945"
     CDS             complement(1657..>2622)
                     /locus_tag="VGM46_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020091188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY4192168.1"
                     /translation="EVDAMALRAIADFSVPALERAGRRHPTARLLSDWRDLIEDPAID
                     AVVVATPVSTHFDIALAALKAGKHVLVEKPMTDSVFKASLLVEEAAKRSLVLMVDHTF
                     IYTGAVRSIHDLIEQGRIGDIYYYDSIRVNLGLFQKDVNVIWDLAAHDFAILDYLVKS
                     KPVAISASAAAFLPDRPENMAHLSVYFDNGAMAHLNVNWMAPVKVRQTLIGGSQRMVI
                     YDDMQNSEKVKVYDRGVDLTGSGDSLREQLISYRMGEMWAPAVSPKEALITEIEQFIR
                     SIDTAECSPSDGESGLRVVEMLAAATRSTVLRGQPIELGNLRNAS"
BASE COUNT          495 a          828 c          836 g          463 t
ORIGIN      
        1 cgaggccgta catgtcgttg aaggagcgca gcagcccttc cccgaacgac ttggccgcgc
       61 catagagcgt gcggttgcca tagggatggt ggctttccgt cgtcggaaaa tcctcggcca
      121 tgccgtagat cgacgccgac gaggccatga cgaccttgcg aaccttcgcc tccacgcatt
      181 ggcgcagcaa gcgataggtg gcatcaacca tcacctgcat ggcttcgtcc ggctcggcgg
      241 cgcaatgggt gatgcgcagc gccgcctgat ggaataccgt gtcgcagcca gcgactagcg
      301 tctgcatcag ccgctggtcg cgtatgtcgc cattgacgat ttcgacgcgc ccggatggaa
      361 gagccgaaac cagattggca attcggccac gcaccatatt gtcgacaacg acgatcctgg
      421 cgcaatccgc cgcgaccagc tggtcgacga tatgagagcc gacgaaaccc gcgccgccgg
      481 tgacaagaac gcgctttcca cgaagaatgc tcaacgctgg cctcccagtc atcttaagtt
      541 tcatttaggc cgtgcgggcc aaaggctgtg cggtggcttc gttcagcgct tcgacgatcc
      601 tggccacgct ggcgtccgcc agttcgggga acatcggcag cgacagcgtt tcgcgcccaa
      661 gcgcttccga aaccggaaaa tcgcccgcgc catagccgag attggcgtag gccggctgta
      721 aatgcaccgg caccggatag tggatgttgc tggcgacgcc ggcatcgtgc agcgaagcac
      781 gcacatggtc gcggtcgctg acccgcaccg catagacatg acagacatgg tcgaggccga
      841 acggtcccgc cggcagcgaa aggtcgccga acaggccctc atggtaaagg ttggcgatgc
      901 ggcggcgcgc ggcattccag ccatccagca tcggaagctt gacgcccagc accgcgccct
      961 gcaccgcgtc catacggaaa ttgaagccgt ggcggacgtg gttatatttg ccttcctgcc
     1021 cccagtcgcg cagcgaggtc accgccgcgg ccagatcggc gcggttggtg gtgatggcgc
     1081 cgccttcgcc gcaggcgccg aggttcttgc cgggatagaa gctgaagcag ccgatatcgc
     1141 cgaaattgcc ggcgcggacg ccatcgcgct cggcgccgtg cgcctgcgcc gcatcctcga
     1201 tcaccgtcag cccaaagcgg cgggcgaccc cgcagatgga ggtcatgtcg gcaagccgcc
     1261 cgtgcaggtg caccgggata atcgccttcg tccggctcgt caccttttct gcaatcttgt
     1321 cggcgtccat cgtccaggtg gccggatcga catcgacgaa caccggggtc gcgccggcat
     1381 agacgacggc ggctgtcgtc gccacgaagg tcatcggcac ggttatcacc tcgtcgcccg
     1441 gcccgatgcc ggcggcgagc agcgcaagat gcagcgcgga ggtgccggaa ttgagggcga
     1501 ccgcgtggcc ggtgccgcaa taggcggcaa agtcccgctc gaactgttcg acgcggtcgc
     1561 cgagcacgaa attgcattcg cgcatggcaa aaaccacggc ctgctccagt tgcgggccaa
     1621 tggactggta ctgggcgcga agatcgagaa atgggatcat gatgcgtttc tcaaattgcc
     1681 aagctcgatc ggctgcccgc gcagaacggt ggagcgcgtg gcggcggcca gcatctcgac
     1741 cacccgcagg ccgctttcgc cgtcggaggg cgaacattcg gcggtgtcga tgctgcggat
     1801 gaactgttcg atttcggtga tgagcgcttc cttcggcgaa acggccggcg cccacatttc
     1861 gcccatgcga taggaaatga gctgttcacg caggctgtcg ccgcttccgg tgaggtcgac
     1921 accgcggtcg tagaccttca ccttctcgct gttttgcatg tcgtcataga tgaccatgcg
     1981 ctggctgccg ccgatcagcg tctggcgaac cttgaccggg gccatccagt tgacgttgag
     2041 atgcgccatc gcgccattgt cgaaatagac cgacagatgc gccatgtttt ccggcctgtc
     2101 gggtaggaag gcggcggcac ttgccgagat ggccaccggc ttcgacttca ccagataatc
     2161 gagaatggcg aaatcatgcg ccgccaggtc ccagatgacg ttgacgtcct tctggaacag
     2221 gccgagattc acgcggatgg agtcgtaata gtagatatcg ccgatccgcc cctgctcgat
     2281 gaggtcgtgg atgctgcgga ccgcgccggt atagatgaag gtatggtcga ccatcagcac
     2341 gagcgagcgc ttggcggctt cctcgaccag cagcgatgcc ttgaagacgc tgtcggtcat
     2401 cggcttttcg accagcacat gcttgccggc cttcagcgcg gccagcgcta tatcgaaatg
     2461 cgtgctcacc ggcgtcgcaa ccaccaccgc atcgatggcc gggtcttcga tcaggtcgcg
     2521 ccagtcgctg agcaagcggg cggtcggatg acggcgtccg gcgcgttcga gcgccggcac
     2581 cgagaaatcg gcaatggcgc gcaaggccat cgcatcgacc tc
//
LOCUS       DASYCS010000183         8534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_331443,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000183 DASYCS010000000
VERSION     DASYCS010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 8534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8534
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331443"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..550
                     /locus_tag="VGM46_05950"
     CDS             62..550
                     /locus_tag="VGM46_05950"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY4192169.1"
                     /translation="MFIRTAGERDLAAVRDLLAETWHATYDPLYGVDKVTEITEARHS
                     VAMLRKMLARPFSEFVVADSGAGLAGLAHAAMLGEERQIVELHEFNVRPAMQGKGIGG
                     LLLEEVEGSFFECRLMRLDVEERNSRAVAFFEAAGFVIAGRGPAKQFVDATLLTLEKP
                     LA"
     gene            664..1059
                     /locus_tag="VGM46_05955"
     CDS             664..1059
                     /locus_tag="VGM46_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010257 - NADH dehydrogenase complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase subunit NDUFA12"
                     /protein_id="HEY4192170.1"
                     /translation="MKSLLQFFTWWNGQTLGTRFHTWRHGKRVGQDALGNVYYEGGVN
                     SDGLTRRWVIYNGYSEASAIPPGWHGWIHHRVDVAPPNEDYKPRDWQKPHELNLTGSG
                     AAYRPKGSVLGNQHRPQVTGDYDAWTPGG"
     gene            1280..1747
                     /locus_tag="VGM46_05960"
     CDS             1280..1747
                     /locus_tag="VGM46_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2155 domain-containing protein"
                     /protein_id="HEY4192171.1"
                     /translation="MSFPGRPMTTGLAAAIAVLALSVTTYAAANPPPAVAAPAKGDRI
                     ANPVAEFAGLDKITGRIISFDVYVDETVQFGALQVTPRVCYSRPEAEEPKTDSFVEVD
                     EITLDRKIRRIFTGWMFAESPGLNAVEHAVYDVWLKACKQKTDVPKPDQAKGT"
     gene            complement(1751..2368)
                     /gene="aat"
                     /locus_tag="VGM46_05965"
     CDS             complement(1751..2368)
                     /gene="aat"
                     /locus_tag="VGM46_05965"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008875589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEY4192172.1"
                     /translation="MTRPYAPGYRIPADLLLKAYASGVFPMAESASDPEVFWVRPERR
                     GIIPLDAFHLPKSLRKTIRKGLFEIRFDFDFESIIDACAERRLERSSTWINAPIREAY
                     IGLHRMGHCHSVEAWREGRLVGGLYGLALGRVFFGESMFSRETDASKVCLAALVERLN
                     QRDFALLDTQFTTDHLKHFGAIDVPRAKYEKMLAQALKGTAVFYP"
     gene            complement(2384..3730)
                     /gene="accC"
                     /locus_tag="VGM46_05970"
     CDS             complement(2384..3730)
                     /gene="accC"
                     /locus_tag="VGM46_05970"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007008043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEY4192173.1"
                     /translation="MFQKILIANRGEIALRVLRACKELGIQTVVVHSTADADAMHVRL
                     ADESVCIGPPPARDSYLNIHQIVAACEITGADAVHPGYGFLSENAKFADILAAHNITF
                     IGPTGDHIRIMGDKIEAKRTAKRLGIPVVPGSDGAVSEEADAKRIASDIGYPVIIKAT
                     AGGGGRGMKVAHSEQDLIEALQTASKEAGAAFGNDAVYIEKYLEKPRHIEVQVFGDGF
                     GNGVHFGERDCSLQRRHQKVWEEAPSPALNAEERARIGGICAKAVADLGYSGAGTIEF
                     LYENGEFYFIEMNTRLQVEHPVTEAITGVDLVHEQIRVASGGGLSVRQEDIRFQGHAI
                     ECRINAEDARTFVPSPGTITHFHTPGGLGIRVDSGVYSGYRIPPYYDSLIGKLIVHGR
                     NRIECMMRLRRALDEFVVDGINTTLPLFRDLVGNADIANGDYDIHWLEKYLAAKTD"
     gene            complement(3751..4215)
                     /gene="accB"
                     /locus_tag="VGM46_05975"
     CDS             complement(3751..4215)
                     /gene="accB"
                     /locus_tag="VGM46_05975"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006333181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEY4192174.1"
                     /translation="MTTKKTGVDQQLVRDLAGILNDTNLTEIEVELGDLKVRVSRQAP
                     AVHAIAAPQGYAPAAPQAAASVAAPAAAPVDISKNAVTSPMVGTAYLSPSPDAKAFVE
                     VGQSVKEGQTLLIIEAMKTMNQIAAPRAGTVKAIFVEDAQPVEYGMPLVVIE"
     gene            complement(4240..4674)
                     /gene="aroQ"
                     /locus_tag="VGM46_05980"
     CDS             complement(4240..4674)
                     /gene="aroQ"
                     /locus_tag="VGM46_05980"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEY4192175.1"
                     /translation="MKTILVLNGPNLNMLGKREPGIYGSKTLDDVAAECRQAASSLGI
                     EVDFRQSNHEGDLVGWIQAASETASGVVLNAGAYTHTSIALHDAIAAVAPLPVVEVHL
                     SNIHARESFRHKSLVAPHAVGMICGFGSLGYTLALQALAARI"
     gene            complement(4820..5593)
                     /locus_tag="VGM46_05985"
     CDS             complement(4820..5593)
                     /locus_tag="VGM46_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEY4192176.1"
                     /translation="MSRKIFLGATAALACAMLVFGLVGAGPRTASAETASKIDRTEVE
                     AIVRDYLLKNPEVLLEVQQALETKQQEAQRIAHLGVINDSKAEIFNSAFDGVVGNPQG
                     KVTIVEFYDYNCGYCKRAIDDMKALTKSDPDLRFVLKEFPILGPDSQKASVVSMAFHK
                     MMPEKYGEFHNALLGGQGRATEGAAIKVALSLGANEAKLREGMKDPTIVEAISQTYDL
                     ANKLAITGTPSYVVGDEVVFGALGQEVLAEKIAAVKASL"
     gene            complement(5644..7002)
                     /locus_tag="VGM46_05990"
     CDS             complement(5644..7002)
                     /locus_tag="VGM46_05990"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008875594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEY4192177.1"
                     /translation="MHGLAVLALGVVLAASGALTALAQGVPIVRDAEIEALVRDYARP
                     IFKAAGLSNAGIQIVLVNDPSFNAFVTGRRLFINTGALMQAETPNEIIGVIAHECGHI
                     AGGHQQRLRDQLERSKTIAIVAGLLGAGAIVAGATSNNKGLAGAGMGIATGGGEVAQR
                     TLLTYQRGEEITADRSAVTYLNATGQSGKGMLKTFARFQSALSLSGAQVDPYRISHPM
                     PQDRISNLENLIQKSQYYDKEDPESLQQRHDMMRIKIAVYTQGQAAAARMIRKSTDGL
                     AARYGEAQSAYLFGNPRSALAKTDALIKVLPKNAYFQELRGDIMMKVNKPKEAAAAYG
                     KAVSLDPSKSGLLMVSDGQALMAIGSPVSLKEAIVKINNGLERDRENSAGYRYLAQAY
                     GELGDIANAELATAEGHYYSGAYQDAKIFAARAQQKFKRGEPGWVRAQDIINYRASGK
                     KN"
     gene            7077..7394
                     /locus_tag="VGM46_05995"
     CDS             7077..7394
                     /locus_tag="VGM46_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192178.1"
                     /translation="MESRRRKVPELVILFRHLCGIAGPGVSHGASMANRIATAGIGLR
                     GIGSRHGLETAMPSPAFGLMRVGVLHFCGGEAVSISGLPLRGVKEKPALSFRQRGFFI
                     HGS"
     gene            complement(7407..>8534)
                     /locus_tag="VGM46_06000"
     CDS             complement(7407..>8534)
                     /locus_tag="VGM46_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HEY4192179.1"
                     /translation="TGHVRSDSSVALAVVRAIEEFLLKDSKCHITVRTPAATALYVLN
                     HKRGTLVELESRFGLTVTIEADDTVGAQLYAISRGLVAEKPAGFVETRALPVYDEPEE
                     PEIVEEEDDEASEPVAEATPRQPDQAARPTEEGEGRDRKRRRRRRRRGGKDRENGDAS
                     NQDAAADAGDDAVSDEAGYADTEQDGDAPLEATAATEAEASEEAQAKKRRRGKRGGKR
                     SRQDDVEANAEGEAEAVAAPASDIAAEASEALIASEEPVIAPAADSDGAPAAEKPKKP
                     RRAAKPKKADAEVVSESETAGTAQAPSEVSVAPPAEEKPAKPRASRRKPAAAAVEPAA
                     AVEKVAEPVVSSTVADAPAEPEAQAEEKPKRAGWWQRKGFF"
BASE COUNT         1482 a         2572 c         2738 g         1742 t
ORIGIN      
        1 gcaaggccaa tccgcaggcc gtcaacgatc tggtcaaggc caagctgggc atcgaatagg
       61 catgttcatt cgcacagctg gcgaacgcga tctggcggcg gtgcgcgacc tgctggccga
      121 gacatggcac gcgacctacg atccgcttta cggcgtcgac aaggtcacgg aaatcaccga
      181 ggcaaggcat tcagtggcga tgctgcgcaa aatgctggcg cggccttttt ccgaattcgt
      241 cgttgccgac agcggcgctg gtcttgccgg cctggcgcat gccgcgatgc tgggcgagga
      301 acgccagatc gttgaactgc acgagttcaa cgtgcgcccg gccatgcagg gcaaggggat
      361 aggcgggctg ctgctggaag aagtcgaagg ctcgttcttc gaatgccgcc tgatgcggct
      421 ggatgtcgag gagagaaaca gccgtgcggt ggccttcttt gaagcggccg gtttcgtgat
      481 cgctggccgc ggcccggcaa agcagtttgt cgacgccacg ttgttgacgc tggaaaagcc
      541 attggcatga cgatgccgct tttttgcggc ggcatttcgc ctggcgcaat cgcaaaaccc
      601 ttgccttcgc ggcgcgccga cgccataagc aggcaacgag aattcgccgc cgcgaggata
      661 ggcatgaagt cactcctgca atttttcacc tggtggaacg gccagacctt gggaacgcgc
      721 ttccatacgt ggcggcacgg caaaagggtc ggccaggacg ccttgggcaa tgtttattac
      781 gaaggcggcg tcaattcgga cgggctgacc cggcgctggg tgatctataa cggctattcg
      841 gaggcctcgg ccattccgcc gggctggcat ggctggatcc atcaccgcgt cgatgtcgcg
      901 ccgcctaatg aagactacaa gccgcgcgat tggcaaaagc cgcatgagct taatctcacc
      961 ggtagcggcg ctgcctaccg gccaaagggt tccgtgctcg gcaatcagca tcgtccgcag
     1021 gttaccggcg attacgatgc ctggacgccg ggtggctgag gggtcgccga cttttttgcc
     1081 tgacaagccg ggcattactt gacgtatctt cgaggccaat gtgcattgcg ctgctcttga
     1141 cgcgaacccg gtctcgcaat ggtgaattcc aaacggtgtt ttatcgccac aattgacgtt
     1201 taggcgcttg ctgatccgac aggggggaat agccttggcg gtacggaatc accccggacg
     1261 caaactaccg gtatgctgca tgagctttcc tggccgaccc atgacaaccg gacttgccgc
     1321 ggccattgcc gttctggccc tgtccgtcac gacatatgct gccgcaaacc cgccgccggc
     1381 ggtcgccgcg cccgccaagg gcgatcgcat cgccaatccc gtggccgagt tcgccgggct
     1441 ggacaagatc accggacgga tcatttcgtt tgacgtttat gtcgatgaga cggtgcagtt
     1501 cggcgccctg caagtaacgc cgcgcgtctg ctattcacgg ccggaagccg aagagccaaa
     1561 aaccgattcg tttgtcgagg tcgatgaaat cacgctcgac cgaaaaatcc gccgcatctt
     1621 caccggctgg atgtttgccg aaagccccgg cctcaacgcg gtcgagcacg cggtctatga
     1681 cgtttggctg aaggcctgca agcagaagac cgatgtgccg aagccggatc aggccaaggg
     1741 cacctgaaat ctacgggtaa aagacggcag tacctttaag agcctgcgcc agcatttttt
     1801 catatttggc gcgcggcacg tcgatcgcgc cgaaatgttt cagatggtcg gtggtgaatt
     1861 gcgtgtccaa gagcgcgaaa tcgcgctgat tcagccgctc gaccagggcc gcgaggcaga
     1921 ccttggaggc atccgtctca cgcgaaaaca tgctttcgcc gaagaagacg cggcccagcg
     1981 ccagaccata gagaccgccg accagccggc cttcgcgcca ggcctcgacc gagtggcaat
     2041 ggcccatgcg gtgcagcccg atataggctt cgcgtatcgg cgcgttgatc caggtggagc
     2101 tgcgttcgag acgcctttcg gcgcaggcgt cgatgatcga ctcaaaatcg aagtcgaagc
     2161 ggatttcgaa caggcccttc cggatcgtct tgcgaaggct tttcggcagg tgaaaggcat
     2221 cgagcgggat gatgcctcgc ctttccggcc gcacccagaa cacttcggga tcggaagcgc
     2281 tttccgccat gggaaacacg cctgaggcat aagccttcag gagaagatcc gcagggatgc
     2341 ggtaaccggg cgcgtaagga cgggtcatcg cgcctggaaa gccttagtcg gtcttggcgg
     2401 ccagatattt ctccagccaa tggatatcgt agtcgccatt ggcgatgtcg gcgttgccga
     2461 cgaggtcgcg gaacagcggc agcgtggtgt tgatgccgtc gacgacgaat tcgtcgagcg
     2521 cgcgccgcag tcgcatcatg cattcgatgc ggttgcggcc atgcacgatc agcttgccga
     2581 tcaggctgtc gtaatagggc ggaatgcgat agccggaata gacgcccgaa tcgacgcgaa
     2641 tgccaaggcc gccgggcgtg tggaaatgcg tgatcgtgcc cggcgaggga acgaaggtgc
     2701 gcgcatcctc ggcgttgatg cggcattcga tggcgtggcc ctggaagcgg atgtcttcct
     2761 gcctgaccga aaggccgccg ccggaggcga cacggatttg ctcatggaca agatcgacgc
     2821 cggtgatcgc ctcggtcacg ggatgttcga cctgaagccg ggtgttcatt tcgatgaaat
     2881 agaactcgcc gttctcgtaa aggaactcga tggtgccggc gccggaatag ccgaggtcgg
     2941 ctacagcttt ggcacagatg ccgccgatgc gggcgcgttc ttcggcattg agcgccggcg
     3001 agggggcttc ctcccacact ttctggtgac gccgctggag cgaacaatcg cgctcgccga
     3061 aatgcacgcc attgccaaag ccgtcgccga acacctggac ttcgatatgg cgcggttttt
     3121 cgagatattt ctcgatatag accgcatcgt tgccgaaggc agcgccggct tccttgctgg
     3181 ctgtctgaag cgcctcgatc aggtcctgtt cgctgtgcgc caccttcatg ccgcgaccgc
     3241 cgccgccggc cgtcgccttg atgatgacgg gatagccgat gtcgcttgca atgcgcttgg
     3301 cgtccgcttc ctcgctgacc gcgccgtccg aaccgggcac gaccggaatg ccgaggcgct
     3361 ttgccgtgcg cttggcttcg atcttgtcgc ccattatacg gatatggtcg cctgtcgggc
     3421 cgatgaaggt gatgttgtgc gcggccagta tatcggcgaa tttcgcgttt tccgacagga
     3481 agccgtagcc gggatgcacg gcatccgcgc cggtgatctc gcaggcagcg acgatctggt
     3541 gaatgttgag atagctgtcg cgcgccggcg gcgggccgat gcacacgctt tcgtcggcaa
     3601 gacgcacatg catggcgtcg gcatcggcgg tggaatgaac cacgacggtc tggatgccga
     3661 gttccttgca ggcccgcagc acgcgaaggg cgatttcgcc gcgattggct atgaggattt
     3721 tctggaacat ggttatgccc gttcccggcc ttattcgatc acgacgagcg gcatgccgta
     3781 ctcgaccggc tgggcgtcct caacgaaaat ggccttgacg gtgccggcgc ggggcgcggc
     3841 aatctggttc atcgtcttca tcgcctcgat gatcagcagc gtctggcctt ccttgacgga
     3901 ctggccgacc tcgacgaacg ccttggcatc gggcgagggc gagagatagg ccgtgcccac
     3961 catgggcgag gtgaccgcgt tctttgaaat gtcgacgggc gctgcggccg gcgcggcaac
     4021 ggaggccgcc gcctgcggcg ctgccggggc gtagccctga ggggcggcga tggcgtgaac
     4081 cgccggcgcc tggcgcgaga cgcgcacctt gaggtcgccg agttccacct cgatctcggt
     4141 caggttggtg tcgttcaaga tgcctgcgag atcgcggacc aactgctggt cgacgccggt
     4201 tttctttgtc gtcatggggg agctttcttt agtcattgct cagatcctgg ctgccagtgc
     4261 ctgaagtgcc agcgtatatc cgagtgatcc gaaaccgcaa atcatgccca ctgcatgagg
     4321 tgcaaccaga gatttgtggc ggaaagattc gcgggcgtga atgttggaga gatgcacttc
     4381 cacgaccggc agcggcgcta cggccgcgat ggcgtcgtgg agggcgatcg aggtgtgcgt
     4441 ataggcgccg gcattgagca ccacgccgct ggccgtctcg cttgcggcct gtatccagcc
     4501 gaccaggtcg ccctcatggt tagactgccg gaaatcgacc tcgatgccca gcgaggacgc
     4561 ggcctgccgg cattcggcag cgacatcgtc gagcgtcttc gacccgtaga tacccggctc
     4621 acgtttgcca agcatgttca ggttcgggcc gttcaggacg agaatcgttt tcaaaaatgc
     4681 cgacctttcc gcgcgccgcg ataattggcg cgcaagtcct acgctctata aagggcatag
     4741 tcggctgcga aaagagcgca agtcatgttc ttgcgctgtc cacagcagcc attgcccggc
     4801 aataccggcc ggatgccgac tatagggaag ccttcacggc ggcgatcttc tcggccagca
     4861 cttcctggcc aagcgcgccg aacaccactt cgtcgccgac gacgtaggaa ggtgtgccgg
     4921 taatggcaag cttgttggcc agatcatacg tctgggagat ggcctcgacg atcgtcgggt
     4981 ccttcatgcc ttcacgcaac tttgcctcat tggcgcccag cgaaagcgcg accttgatgg
     5041 ccgctccttc cgtggcgcgg ccctggccgc cgagcagtgc gttgtggaat tcgccgtatt
     5101 tttccggcat catcttgtgg aatgccatcg aaacgacgct ggccttttgc gaatccggcc
     5161 caagaatggg gaattccttg agcacgaagc gcaggtccgg atcggacttg gtcagcgcct
     5221 tcatgtcgtc gatggcgcgt ttgcagtagc cgcaattata gtcatagaat tcgacgatgg
     5281 tgaccttgcc ttgcgggttg ccgacgacgc cgtcaaaggc ggagttgaag atttcagcct
     5341 tcgaatcgtt gatgacgccc aaatgggcga tgcgctgcgc ctcttgctgc ttggtttcaa
     5401 gcgcctgctg gacttcgagc agcacttcgg ggttcttgag caaatagtcg cgcacgatgg
     5461 cttcgacctc ggtgcggtcg atcttggacg cggtttcggc gctggccgtt cgtggcccgg
     5521 cgccgaccaa cccgaatacc agcatggcgc aggcaagtgc cgcggtggcg cccagaaaga
     5581 tttttctgct catggtcttt cccttcaagc ccgttcctgc cgcacaatct gcgcccggaa
     5641 ggttcaattc ttcttacccg atgcccggta gtttatgatg tcttgcgccc gcacccaacc
     5701 gggttcgccg cgtttgaatt tctgttgggc gcgcgccgcg aatatcttgg catcctgata
     5761 ggcgccggaa taataatggc cctccgcggt tgccagttcc gcattcgcga tgtcgccgag
     5821 ttcgccatag gcctgcgcca gatagcggta gccggcggag ttttcacggt cccgctcaag
     5881 gccgttgttt atcttgacga tagcttcctt gagcgagacg ggcgagccga tcgccatcaa
     5941 ggcctggccg tccgaaacca tcagaagacc ggatttcgaa gggtcgaggc tgacggcctt
     6001 gccataggcc gccgccgctt ccttcggctt gttgaccttc atcatgatat cgccgcgcaa
     6061 ttcctggaag taggcgtttt tcggcagcac ttttatcagc gcgtcggtct tggccagcgc
     6121 cgagcgggga ttgccgaaca gataggccga ctgggcttca ccatagcgtg cggcgaggcc
     6181 gtccgtgctc ttgcggatca tccgcgccgc cgccgcctga ccctgcgtat agacggcgat
     6241 cttgatgcgc atcatgtcgt ggcgctgttg cagggattcc gggtcttcct tgtcgtaata
     6301 ctggcttttc tggatcagat tttccagatt cgagatgcgg tcctgcggca tcgggtggct
     6361 aatccggtat ggatcgacct gcgcgcccga aagcgacagt gcgctctgga aacgcgcaaa
     6421 cgtcttcagc atgcccttgc ccgactgacc ggtggcgttg aggtaggtga cggcggagcg
     6481 gtccgccgtt atctcctcgc cgcgctggta ggtgagcagc gtgcgttggg cgacctcgcc
     6541 gccgccggtg gcgatgccca tgcctgcgcc cgcgaggccc ttattgttgc ttgtcgcgcc
     6601 ggccacaatg gcgcccgcgc caagcaaccc ggcgacgatt gcgatggtct tggatcgttc
     6661 gagctggtcg cgcagccgct gctggtggcc acccgcgatg tggccgcact catgcgcgat
     6721 gacgccgatg atctcgttgg gggtttcggc ctgcatcagc gcgccggtgt tgatgaacag
     6781 gcggcggccg gtgacaaagg cgttgaagct aggatcattg accagcacga tctggatgcc
     6841 ggcgttggaa aggcccgccg ccttgaagat cggccgcgca tagtcgcgca ccaacgcctc
     6901 gatctcggcg tcgcgcacga tcggcacgcc ctgcgccaga gcggtgagcg cgcctgacgc
     6961 cgccagcacg acaccgagcg caagcacggc tagcccgtgc atcgctttgg cgatggtcga
     7021 tttcggacgt ggatggctca acatggtgcg tccactggta aatgaggtgg cggcagatgg
     7081 aaagccgacg ccgaaaagtt cctgaacttg tgatcctatt ccggcattta tgcggcatcg
     7141 ccgggccggg tgtcagccac ggggcgtcca tggccaaccg cattgccacg gcaggtattg
     7201 gcttacgagg aatcggctca cggcatggat tggagacggc gatgccgtcg ccggctttcg
     7261 ggctgatgag ggtcggcgta ctgcattttt gcggcggcga ggcggtatca atatcgggat
     7321 tgccgctacg tggcgtcaaa gaaaaacccg cgctgtcttt caggcagcgc gggtttttca
     7381 ttcatggatc atgaagaccg gaagtctcag aagaaaccct tccgctgcca ccagccggcg
     7441 cgcttcggtt tttcttccgc ctgtgcctca ggctctgccg gcgcatctgc cacggtcgag
     7501 gaaacgaccg gctcggccac cttctctact gcggcagcgg gctctactgc ggcggcagca
     7561 ggcttgcgtc gggaggcgcg gggctttgcc ggtttttcct cggcaggagg ggccaccgaa
     7621 acctcggacg gcgcttgcgc ggtgcccgcc gtctccgact ccgagaccac ctcggcgtcg
     7681 gcctttttcg gtttggcggc gcggcgcggc tttttcggct tctcggcggc gggtgcgcca
     7741 tcgctgtcgg cggcaggcgc gattacgggc tcttcggatg cgatgagggc ttccgaagct
     7801 tccgccgcga tatctgatgc cggagcggca accgcctcgg cttcgccttc cgcattggcc
     7861 tcgacgtcgt cctgacggct gcgcttgccg ccgcgcttgc cgcgccgacg tttcttggcc
     7921 tgtgcttctt ccgatgcttc ggcttcggtg gcggccgttg cttctagcgg cgcatcgcca
     7981 tcctgttccg tatcggcgta accggcttca tccgacacgg cgtcatcgcc tgcatcggct
     8041 gccgcatcct gattggaggc gtcgccgttt tcgcgatcct tgccgccacg acgccgacgc
     8101 ctgcggcggc gcttgcggtc gcggccttcg ccttcttccg tcggccgggc cgcttgatcg
     8161 ggctgacgcg gcgttgcctc ggccaccggt tccgatgcct catcgtcctc ttcctcgacg
     8221 atttcgggtt cctcaggttc gtcatagacg ggcagggcgc gtgtctcgac gaagccggca
     8281 ggcttttcgg cgaccaggcc acgcgaaatc gcgtaaagct gggcgcccac cgtgtcgtcg
     8341 gcttcgatgg tgacggtcag gccgaagcgg ctttccagct cgaccagcgt gccgcgcttg
     8401 tggttaagca cgtagagcgc cgttgcagcg ggtgtgcgca cggtgatgtg gcacttggaa
     8461 tccttgagca ggaattcctc gatggcgcgc acgacggcca gtgccaccga cgaatccgag
     8521 cgcacatggc cggt
//
LOCUS       DASYCS010000184         4084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4248082,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000184 DASYCS010000000
VERSION     DASYCS010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4248082"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..137
                     /locus_tag="VGM46_06005"
     CDS             <1..137
                     /locus_tag="VGM46_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015397569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEY4192180.1"
                     /translation="IDVIGDYMTEINVTSPTGIREIKRFGGADIAGLFWDAVEDKRRS
                     "
     gene            355..1887
                     /locus_tag="VGM46_06010"
     CDS             355..1887
                     /locus_tag="VGM46_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012091020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEY4192181.1"
                     /translation="MVSRVRTVAFQGIEAVPVDVQVMVAPGKVGMSIVGLPDKAVAES
                     RERVQAALHASGLSMPARKVTINLAPADLPKEGSHYDLPIALGLMAALGAVPGDMLAG
                     YVVLGELSLDGTIAPVAGALPAAIGANAEGKGLICPFACGPEAAWAGSDIDILAPRSL
                     IAIANHFRGTQVLSRPEPGMHAAAGNLPDLADIKGQETAKRALEVAAAGGHNLLMVGP
                     PGSGKSMLAQRLPSILPPLMPRELLEVSMIASVAGELGEGKLTDRRPFRSPHHSASMA
                     SMVGGGMRARPGEVSLAHNGVLFLDEFPEFSPQTLDALRQPLEDGECMIARVNHRVTY
                     PARIQLVAAMNPCRCGMAGEPGYRCARGERCRTDYQARISGPLLDRIDLRIEVPAVSA
                     SDLIRPDRAENSAAVALRVARARTIQRERFERLGIAKATTNAHCSASVIEAIAALDAA
                     AMALLTDASEKLGFSARAYHRLLKVARTLADLEASETVGRLHLAEAISYRMNSERLAR
                     AA"
     gene            complement(2031..2915)
                     /locus_tag="VGM46_06015"
     CDS             complement(2031..2915)
                     /locus_tag="VGM46_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003527002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY4192182.1"
                     /translation="MKTPPPLNHIRSFECSARHLSFTSAATELGYTQAAVSNHVRALE
                     QYIGRPLFVRHPRSLKLTEAGEAFLPTLRQALDQIDFATDAIVASARNRTVVISCPTS
                     LAENWLAGCIAGFRAEHPEIELLVHGTLWEDASEPAADITISIRRFDDRPPDGIALWT
                     DRLALLCAPRLLDGPNGISKPGDILSLDRIFIHGRQEYWTCMGEALGIPATAHEKGIA
                     TNASNVALELAAAGGGCVAMQISLARIYLQRGLLVEPFAVRAASPWSYYLSENKLSKG
                     SAVKLARDWIVSRAKEME"
     gene            complement(3052..>4084)
                     /locus_tag="VGM46_06020"
     CDS             complement(3052..>4084)
                     /locus_tag="VGM46_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006200653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY4192183.1"
                     /translation="PVDDNPKEFELIMPDYRLRAAMLAYSRTVAFVSLLISLFTASLV
                     YVAIDRIMIRPMRAMTRSMLSFSEAPDDPARIISPSARADEIGIAERELSLMQERLQK
                     MLAEQKHLADLGLAVSKINHDMRNILASAQLMSDRLRQVKDPTVQAYAPKLLRALDRA
                     VSYSEGVLAYGRTQEPAPSRRRIRLRQLVEEVHSLLNMENGIEFENTVEPAFEVDADA
                     EQLFRVLTNLCRNSVQAMAGDTEGALVRRLTMAAERQGTVSRILVSDTGPGLPAKARE
                     NLFMAFHGSARSGGTGLGLAVAQELVRAHGGVVELVESIGGRTTFSVSIPDQPARLDQ
                     LRNGLRRPA"
BASE COUNT          680 a         1317 c         1335 g          752 t
ORIGIN      
        1 gcatcgacgt catcggcgac tacatgacgg aaatcaacgt cacctcgccg accggcatcc
       61 gcgagatcaa gcgttttggc ggcgcagata tcgccggcct gttctgggac gccgtcgagg
      121 acaagcgccg cagctaggcg cacttcatct acgccaggcc ttggccggcc gccgcatgcg
      181 cggccgcata agccggcgtg gccctgcgct ttgttccagc catgcaacct ggcacaaccg
      241 catcgcatcc tggccgtttg attgttctgg taatgttctt gctttggccg cgcgaatgtg
      301 cttgtctggc cttagaggaa cggcgcaggg gatcgtcggc ctcgggggca tggaatggtt
      361 tctcgcgttc gcaccgtggc gttccaaggc atagaggccg tgccggtcga tgtgcaggtg
      421 atggtggcgc ccggcaaggt cggcatgtcg atcgtcggcc tgcccgacaa ggcggtggcc
      481 gaaagccgcg agcgggtgca ggccgccctt cacgcatccg gcctgtcgat gcccgccagg
      541 aaggtcacaa tcaacctcgc gcccgccgat ctgcccaagg aaggcagcca ctacgatttg
      601 ccgattgcac tcggcctgat ggcggcgctc ggcgcggttc ccggcgacat gctggccggt
      661 tatgtcgtgc tgggcgaatt gtcgctggat ggcaccatcg ccccggttgc cggcgcgttg
      721 ccggcggcaa tcggggccaa cgccgagggc aagggcctca tctgtccctt cgcctgcggg
      781 ccggaagcgg cttgggccgg atcggacatc gatattctgg cgccgcgcag cctgattgcc
      841 attgccaacc attttcgggg cacgcaggtt ttgtcgcggc cggaacccgg catgcatgcc
      901 gccgccggca acctgcccga ccttgccgat atcaaggggc aggaaaccgc caagcgcgcg
      961 ctcgaggtgg cggcggccgg cgggcataat cttttgatgg tcgggccgcc cggctcgggc
     1021 aagtccatgc tggcgcagcg gctgccctcg atcctgccgc cgctgatgcc aagggaattg
     1081 ctggaagttt cgatgatcgc ctcggtcgcc ggcgaactgg gcgagggcaa gctgaccgac
     1141 cgccgcccgt tccgttcgcc gcaccattca gcctcgatgg cctcgatggt cggcggtgga
     1201 atgcgcgcgc ggcccggcga agtgtcgctt gctcataatg gcgtgctttt cctcgacgaa
     1261 ttcccggaat tttcaccgca gacgctggat gcgctgcgcc agccgctcga agacggcgaa
     1321 tgcatgatcg cgcgcgtcaa tcatcgcgtc acctatccgg cgcgcatcca gctggttgcc
     1381 gcgatgaacc cctgccgctg cggcatggcg ggtgaacccg gctatcgctg cgcgcgcggc
     1441 gaacgctgcc gcaccgatta ccaggcgcgc atttcaggac cgctgctcga ccgtatcgac
     1501 ctgcgcatcg aggtgccggc tgtttccgcg agcgacctga tcaggccgga tcgcgctgaa
     1561 aacagcgcgg cggtggccct gcgcgtggcg cgagcgcgca cgatccagcg cgagcgtttc
     1621 gagcgcttgg gtatcgccaa ggcaacgacg aacgcccatt gctcggcttc cgtcatcgag
     1681 gctatagcgg cgctggacgc tgccgcgatg gcgctgttga ccgatgccag cgaaaagctg
     1741 ggcttttcgg ctcgtgccta tcatcgcttg ctgaaagtgg cgcggacatt ggccgatctt
     1801 gaggcgagcg agacggttgg gaggctgcat ctggccgagg cgatttccta tcgcatgaat
     1861 tcggagcgtc tggcccgcgc cgcctgatgg tggccgagtc ttcacaggct gcggcgttcc
     1921 atcacttgct cacactcggc ctataaccgg catggtccgc ttgccgttgt gttcggccag
     1981 aatccgacat catcgttcca ccggttgcct gccgcgcttc aggcgaaaaa ctattccatt
     2041 tccttggccc gcgaaacgat ccagtccctc gccagcttga ccgcgctacc cttggagagc
     2101 ttgttttccg acaaataata gctccatggg ctggccgcgc gaaccgcgaa cggttcgacc
     2161 agcaggcctc tttgcagata aatccgcgcc agtgagatct gcatggccac gcatccgccg
     2221 cccgccgccg ccagttccag cgcgacattc gaggcgttgg tggcgatgcc tttttcatgc
     2281 gccgtggcgg gaatgcccaa cgcctcgccc atgcatgtcc aatattcctg gcggccatga
     2341 ataaagatgc gatcgaggct cagtatgtcg cccggcttgc tgatgccgtt cggtccgtcg
     2401 agcaagcgcg gcgcgcacaa aagagccagc cggtcggtcc acaaggcgat gccgtccggc
     2461 gggcggtcgt cgaaacggcg gatggaaatg gtgatgtctg ccgctggctc gctggcatct
     2521 tcccacaatg ttccatgcac cagcagctcg atttccggat gttctgcgcg aaaacccgcg
     2581 atacaaccgg ccagccagtt ttcagcaagg ctggtcgggc aggaaatcac caccgtccgg
     2641 ttgcgcgccg aagcgacgat ggcatcggtg gcgaagtcga tctggtcgag cgcctggcgc
     2701 agcgtcggaa ggaatgcctc gcccgcttcc gtcagcttga ggctgcgcgg atggcgcacg
     2761 aacagcggcc ggccgatata ttgctccagc gcccggacat ggttgctgac ggcggcctgc
     2821 gtatagccaa gctcggtcgc ggccgaagtg aagctgagat ggcgcgccga acattcgaac
     2881 gagcggatat ggttcagggg cggcggtgtt ttcatctcac gctccgggcc atacgcttcg
     2941 ccatgccaat tccggtaatt gacaaccgtt ctgcccaaac gccagcttgt cacgaaaggc
     3001 agcgcctctt atcccaaaat cgcgaccctt gaagggcatc ctccggcgcc ttcaggccgg
     3061 ccggcgcagg ccgttgcgca gctggtcgag ccgggccggc tggtcgggga tcgacaccga
     3121 gaaggtggtg cgcccgccaa tgctttccac cagttccacc acgccgccat gcgcgcggac
     3181 cagttcctgc gcaaccgcca ggccgagacc ggttcccccg ctgcgcgccg agccgtgaaa
     3241 cgccataaac aggttttcgc gcgcctttgc cggcaggccg ggaccggtat cgctgacgag
     3301 gatgcggctg actgtcccct gccgctccgc cgccatcgtc agccggcgca ccagcgcccc
     3361 ctcggtgtcg cccgccatcg cctggacgga gttgcggcag agattggtga ggacgcgaaa
     3421 cagctgctcg gcgtcggcgt cgacctcgaa ggccggctcg acggtatttt cgaattcgat
     3481 gccgttttcc atgttgagca ggctgtggac ttcttccacc agctggcgga gcctgatccg
     3541 gcggcgcgac ggcgccggtt cctgcgtgcg tccataggcc agcacgcctt cggaatagga
     3601 tacggcgcgg tcgagcgcgc gcagcagctt cggcgcatag gcctgcacgg tcgggtcctt
     3661 cacctgccgc aggcggtccg acatcagctg cgccgatgcc agtatgttgc gcatgtcatg
     3721 gttgatcttc gacacggcga ggccgagatc ggcaaggtgc ttctgctcgg ccagcatttt
     3781 ctgtagccgc tcctgcatca gcgaaagctc gcgctcggcg atgccgatct cgtcggcgcg
     3841 cgccgatggg ctgatgatgc gggcggggtc gtcgggcgct tcggagaagg acagcatcga
     3901 gcgcgtcatc gcgcgcatcg ggcggatcat gatgcggtcg atggcgacat agacgagcga
     3961 ggcggtgaac agcgagatca gcagcgagac gaaggcgacc gtgcgcgaat aggccagcat
     4021 ggcggcgcgc agccggtaat ccggcatgat cagctcgaat tccttgggat tatcgtcaac
     4081 cggg
//
LOCUS       DASYCS010000185         3987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4395047,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000185 DASYCS010000000
VERSION     DASYCS010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4395047"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="VGM46_06025"
     CDS             <1..733
                     /locus_tag="VGM46_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006335897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA protein"
                     /protein_id="HEY4192184.1"
                     /translation="FAARDTQFAFTVANGVLRAPPVKLENKAATLTANIAADLGATTA
                     SAKGSVAYKPGDEALVGSEPVMNFDVEGPLDAVGRTFDSQPLAQFLTQRALEKEQRRV
                     EDMQAVLLEKQRLRREVRYYAALQDAREKAAQERRRLEEMRVKAEVEARAKAAAEAQA
                     KADEEAARIAARAKAQEAARAEAARAEALQKVKPDAPAGAGQGSQVERAPLPPANEVA
                     PVPAQPAVKPNVFSIDNLLKSLDGG"
     gene            complement(816..1046)
                     /locus_tag="VGM46_06030"
     CDS             complement(816..1046)
                     /locus_tag="VGM46_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006204913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HEY4192185.1"
                     /translation="MSTVAKRSITIRGHRTSYSLEKPFYDDLVAIAIHRKMTLAALVA
                     SIDEARAPDTNLSSALRLHVLDWAKRPVSDGN"
     gene            complement(1075..1272)
                     /locus_tag="VGM46_06035"
     CDS             complement(1075..1272)
                     /locus_tag="VGM46_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4169 family protein"
                     /protein_id="HEY4192186.1"
                     /translation="MGDIVNLRMARKRKARAGREQEAEQNRALHGRSRAESKNDRQIA
                     EKAAKFIDGHRLSPPTEGDGK"
     gene            complement(1289..1834)
                     /locus_tag="VGM46_06040"
     CDS             complement(1289..1834)
                     /locus_tag="VGM46_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SspB family protein"
                     /protein_id="HEY4192187.1"
                     /translation="MADDHIRYDILAQEALRGVMRKVLAEVARTGLPGNHHFFITFLT
                     GAPGVRISSRLRERYPEQMTIVVQFQYWDLKVSDTGFEIGLSFSDVPEKLEVPFSAVR
                     GFYDPSVNFELEFDVKTEETAEAGETAGDPPAIAIVSEKKAEPKEAKKKAAEPEKAEK
                     KSDAAPAKGADVVSLDAFRKK"
     gene            complement(1952..2692)
                     /locus_tag="VGM46_06045"
     CDS             complement(1952..2692)
                     /locus_tag="VGM46_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY4192188.1"
                     /translation="METQPIADILPTEGLPTEGISTPDTPTPGLPPRGHEAKRASIID
                     AAGDVFCREGYAGANIDMIAAEAGVSRQTVYNHHHDKEKLFVAVVRDLTERSNAGVFA
                     TLLTFPDQPGDIEADLIAFAVRLNRNCICNREGKFLRKLIQAESERYPELFAPWRNDG
                     PGKTWSALAARFARLAHAGYLQVDDPDVAARQFFSLINADLHMTMMLGGTPTDAEVLE
                     AATNAVRTFLRAFGKTHGQAAQPALADA"
     gene            2850..>3987
                     /locus_tag="VGM46_06050"
     CDS             2850..>3987
                     /locus_tag="VGM46_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019859029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEY4192189.1"
                     /translation="MKPKLLRVAVVLGLLSAIGPFAIDMYLPALPSIGTDLKAGSAAV
                     QMSLLIFFLSMGLGQIVVGPISDMVGRKLPLYVGLAVFIAGGIGAALSPTVEWLIAFR
                     FVQGLGACAGMAVPRAIVRDMHTGAEATRLMSMLMLVFSISPILAPLTGSFIIEAFGW
                     RGVFWAVTGAAVIAVVLLATSLKETRPAEARIGSSLGTALSGYRFLLGQRKFMGLTAI
                     GGFAISSFFVYLSTSSFILINHYGLTPQVYSLFFSINAVSFFAMSQLTGVLSERFGLR
                     RVVRVAVTGFATTMVLLLAVTASGVDRLDVLAALLFVGYGFVGLVVPTTSVLAMDDHG
                     EIAGTASALMGTLQFAIGAVAMGVAGAFFNGTPLPMVAGIALCAV"
BASE COUNT          690 a         1166 c         1316 g          815 t
ORIGIN      
        1 tttcgcggcg cgcgatacgc aatttgcctt tacggtcgcc aatggcgtgt tgcgcgcgcc
       61 gccggtcaaa ctggaaaaca aggcggcgac gctgacggcg aacattgccg ccgacctcgg
      121 cgcaacgaca gcctcggcca agggcagcgt ggcctacaag cccggcgacg aggcattggt
      181 cggctcggaa cccgtgatga atttcgatgt cgaagggccg cttgacgcgg ttggccgcac
      241 gttcgacagc cagccgctgg cgcaattcct gacacagcgg gcgttggaaa aagagcagcg
      301 gcgggtcgag gacatgcagg ctgtgctgct tgaaaagcaa cgactgcggc gcgaggtgcg
      361 ctattacgct gcgttgcagg atgcgcgcga aaaagcggcg caagaacgca ggcggctgga
      421 agagatgcgc gtaaaggcgg aagtcgaagc gcgcgccaag gccgccgcgg aggcgcaggc
      481 gaaagccgac gaggaagccg cccggatcgc ggcgcgggcg aaggcgcaag aggcggccag
      541 ggcggaagcc gccagggccg aggcgctgca aaaggtgaag ccggacgcgc ccgctggcgc
      601 agggcagggg tcgcaggtcg aacgcgcgcc gctgccgccg gcaaatgaag tcgctcccgt
      661 gcccgcccag ccggcggtga aaccaaacgt cttttcgatc gacaatctgc tgaaatcgct
      721 cgacggcggt tgacgacgag aatcagattt tccccctaag gctctgattc taaatcgaat
      781 ctcgacgaaa ttatcggcaa tttatttccg gcagcctaat tgccgtctga aaccgggcgt
      841 ttcgcccaat cgagtacgtg aagcctgagc gcggaggaca ggttggtgtc cggcgcgcgc
      901 gcttcgtcga tgctggcgac aagggcggcc aacgtcatct tccggtggat tgcgatggcg
      961 acgaggtcgt catagaacgg cttttccagc gaataactgg tgcgatggcc gcgaatggtg
     1021 atcgaacgtt ttgcaaccgt gctcatgagc gatgttcacg gcgcgcatcg gcgttcattt
     1081 gccgtcgcct tcggtaggcg gcgaaaggcg gtgcccatcg atgaatttcg ccgccttttc
     1141 cgcgatctgg cggtcatttt tgctttccgc cctggagcgg ccgtgcaagg cgcggttctg
     1201 ttcggcttcc tgctcgcgcc cggcacgcgc cttgcgcttt cgggccatgc ggagattgac
     1261 gatatcgccc attcagcgcg gccggatgtt atttcttgcg gaaggcatcg agtgaaacca
     1321 catcggcgcc cttggcgggt gcggcatccg atttcttttc agccttttca ggttcggcgg
     1381 cttttttctt ggcctccttc ggttcggcct tcttttcgga gacgatcgcg attgccggcg
     1441 gatcgccggc tgtttctccg gcttccgccg tttcctcggt cttcacgtcg aattcgagtt
     1501 cgaaattgac ggagggatca tagaagccgc gcacggcgga aaagggaact tccagctttt
     1561 cgggaacgtc cgagaaggac aggccgatct caaaaccggt gtcggatact ttcaaatccc
     1621 aatactggaa ctggacgacg atggtcatct gctcaggata acgctcgcgc aggcggctgg
     1681 aaatgcgcac gcccggcgcc ccggtcagga aggtgatgaa aaagtgatgg ttgcccggca
     1741 ggccggtgcg cgcgacctcg gccagaacct tgcgcatgac gccgcgcagg gcctcctggg
     1801 ccaaaatgtc gtagcggatg tggtcgtcgg ccatttaaca gtacgatcct ttgaaatctc
     1861 accctagctg taatcaagct tgggggcgac gtaaaccgga tatagatcgc cttggcgctc
     1921 cgtggagagc ctcgctgccg atttttttgg atcaggcgtc cgccaaggcc ggctgcgcgg
     1981 cctggccatg cgttttgccg aaggcgcgca gaaaagtgcg aaccgcattg gtggccgctt
     2041 caagcacttc cgcgtcggtg ggcgtgccgc ctagcatcat ggtcatgtgc agatcggcat
     2101 tgatgaggct gaagaactgg cgcgcggcga catcggggtc gtcgacctgg aggtagccgg
     2161 catgggccag gcgcgcaaag cgggcagcca gcgccgacca agtcttgccc ggaccgtcat
     2221 tgcgccaagg cgcgaaaagc tcggggtagc gctcgctctc cgcctggatc agcttgcgca
     2281 ggaattttcc ttcgcgattg cagatacagt tgcggttcag ccgcacggca aaagcgatga
     2341 ggtcggcctc aatgtcgcct ggctggtcgg gaaaggtcag aagcgtcgcg aaaacgccgg
     2401 cgttcgaccg ttcggtgagg tcgcgcacca cggcgacgaa cagtttttcc ttgtcgtggt
     2461 ggtggttgta gacggtctgg cgcgagacgc cggcttccgc cgcgatcatg tcgatattgg
     2521 cgcccgcata gccttcgcgg cagaaaacgt caccggcggc atcgatgatg gaggcgcgct
     2581 tggcctcgtg cccgcgtggg ggcagccctg gtgtcggggt atccggtgtc gagatgccct
     2641 ctgtcgggag cccctctgtc ggcagaatgt cagcaatcgg ctgcgtttcc atgaaaacca
     2701 tatagagccg gttgacaaat tagacaaggc tgtctaattt tttggataaa gattttcgac
     2761 cttgaaccgt cgaaatcgca aagggcgaac cgtccttggg ttttacgccg cgatgcggcg
     2821 gcaaccacat ctgaaagaag tccacgacca tgaaaccgaa attgctccgc gtcgcggtcg
     2881 tgcttggcct attgtcggcc atcggcccgt ttgccatcga tatgtatttg ccggcgctgc
     2941 cgtcgatcgg caccgacctc aaagccggca gcgccgccgt gcagatgagc ctgttgatct
     3001 tcttcctgtc gatggggctt ggccagatcg tggttggccc gatctccgac atggtggggc
     3061 gcaagctgcc gctctatgtc gggctggccg tattcatcgc cggcggcatc ggcgcggcgc
     3121 tgtcgcccac cgtcgaatgg ctgatcgcat tccgcttcgt gcagggcctc ggcgcctgtg
     3181 ccggcatggc cgtgccccgc gccatcgtgc gcgacatgca caccggcgcg gaagcgacca
     3241 ggctgatgtc gatgctaatg ctggtgtttt ccatttcgcc tattcttgcg ccgctgaccg
     3301 gcagcttcat catcgaggcg ttcggctggc ggggtgtgtt ctgggcggtg acgggtgcgg
     3361 cggtcatcgc ggtcgtgctt ttggccacgt cgctgaagga aacgcggccg gccgaagcac
     3421 gcatcggcag ctcgttgggc acagcacttt ccggctatcg cttcctgctt ggccagcgca
     3481 aattcatggg gctgacggcc ataggcggct tcgccatttc cagcttcttc gtttatctgt
     3541 cgacgtcgtc cttcatcctc atcaaccatt acgggctgac gccgcaggtc tacagcctgt
     3601 tcttctcgat caatgcggtg tcgttcttcg ccatgtcgca gttgaccggg gtgctgtcgg
     3661 agcggttcgg cctacgacgg gtggtgcggg tcgcggtgac cggtttcgcc acgacaatgg
     3721 tgctgctgtt ggccgttacg gcttccgggg tcgaccggct cgatgtgctg gcggcgctgt
     3781 tgttcgtcgg ttacggcttc gtcggcctcg tcgttccgac cacttcggtg ctggcgatgg
     3841 acgaccatgg cgagatcgcc ggaaccgcct cggcgctgat gggaacgctg caattcgcca
     3901 tcggcgcggt tgcgatgggg gtggccggcg cattcttcaa cggcacgccg ctgccgatgg
     3961 tggccggcat cgcgctatgc gcggtga
//
LOCUS       DASYCS010000186         1723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1784279,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000186 DASYCS010000000
VERSION     DASYCS010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1784279"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VGM46_06055"
     CDS             <1..211
                     /locus_tag="VGM46_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007631911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192190.1"
                     /translation="ISRGVFWGPLAYAMMGGILVATVLTILVLPAGYALFFGREPKGR
                     AKPKDAQQSVHTDSETEHLPALAAE"
     gene            complement(224..1168)
                     /locus_tag="VGM46_06060"
     CDS             complement(224..1168)
                     /locus_tag="VGM46_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEY4192191.1"
                     /translation="MANDKISSLRSVASVGECMIELSASASGWQMGHAGDAFNTLWNL
                     RALLPETVDTDFVSAFGTDPFSDQQIAFMASNNIGVAASPRLPLFHPGLYAISLDGAE
                     RSFTYWRSQAVARRLADDAGLLRASLKARQLIYFSGITLAILDDAGRRAFLDAVALAG
                     KEGALIAFDPNYRSRLWASEVDARSAIGDALAYVDIALPTFADEQALHGDHAPHDTAT
                     RLSLAGAKEVVVKDGPEAALVKNAEVSETVPALHVENPLDTTGAGDSFNGGYLAARLL
                     GMEPLAAVERAHRVAAATIQVRGALAPQSMLRSAFEAA"
BASE COUNT          341 a          535 c          542 g          305 t
ORIGIN      
        1 gatctcgcgc ggggtgttct gggggccgct cgcctatgcc atgatgggcg gcattctggt
       61 ggcgacggtg ctgaccatcc tggtgctgcc ggccggctat gcgctgttct tcggcaggga
      121 gccgaaagga cgcgccaagc cgaaggacgc gcagcaaagc gtgcatacgg acagcgaaac
      181 cgaacatctt cccgcgctgg cagccgaata gccggcgcgg gcgctatgcg gcttcgaagg
      241 cgctgcgcag catggattgc ggcgccagcg cgccgcgcac ctggatcgtc gcagcggcga
      301 cgcggtgggc gcgctcgacg gcggcaagcg gctccatgcc cagcaatctt gcggcgagat
      361 agccaccgtt gaaagaatct cccgcgccgg tcgtatcgag cgggttttca acatggagcg
      421 ccggtaccgt ttccgacact tcggcgttct tgaccagcgc cgcctccggt ccatccttga
      481 cgacgacttc ctttgccccc gcaagcgaaa gccttgttgc ggtatcgtgc ggcgcgtgat
      541 cgccatgaag cgcctgctcg tcggcaaaag tgggaagcgc tatgtcgaca taggccagcg
      601 cgtcgccgat cgcggagcgc gcatccacct cgctcgccca cagccttgac cggtagttcg
      661 gatcgaaggc gatgagcgcg ccttccttgc ccgcgagggc gacggcatcg agaaatgccc
      721 gacgcccggc atcatccaga attgccagcg tgatcccgga aaaatagatg agctggcgcg
      781 ccttcaggct tgcccgcaac aggccggcat cgtccgccag ccgccgcgcc accgcctgcg
      841 agcgccaata ggtaaacgag cgctcggcgc cgtcgagcga aatggcatag aggccgggat
      901 ggaaaagcgg caggcgcggg cttgccgcca cgccgatatt gttcgacgcc atgaaggcaa
      961 tctgctggtc ggagaacggg tccgttccga aagccgaaac gaaatcggta tcgacggttt
     1021 ccggcagcaa ggccctcagg ttccataggg tgttgaaggc atcgccggca tggcccatct
     1081 gccaacccga agcgctggcc gacagttcga tcatgcactc cccgacgctc gccacggacc
     1141 gcagggatga gattttatcg tttgccattg cgctttcggg accggtgacg ctggcgttat
     1201 tgcgttgttg ggacgcgggc gcaagaccgg cccacacaac cggcgccgca ggcagacagc
     1261 catttcatgg ccggcttgag cttatcggcc agcggcggca tggcgtcatc gagcgcgagg
     1321 cttcggccag aaaccgacgc cggaaagcga cgcagcagcc ttcgcggaag cgcggattgg
     1381 acggatggat ggccacggct ggccgcgaaa aagaaaaagc cccgcatctg tttgaagatg
     1441 cagggctttt cctgaaattg gtgcccagaa gaggattgcg ctcagtgagc ctatagcctt
     1501 tgatttataa aggaaatttt ctgcgctgtc cacccattgt cacacggtac tgttacacgg
     1561 tccagcctgc cctccaaaca ataggcacac acgttggttt caagtatgtg ccgtctccta
     1621 aaaaattaga attgccctgc atcttcgtac tatcccgaac gccaccccaa gccatccgtg
     1681 ggccagaccg tggggaggat agtacgcatc taccgagtgt ccg
//
LOCUS       DASYCS010000187         4716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_5119329,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000187 DASYCS010000000
VERSION     DASYCS010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4716
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5119329"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VGM46_06065"
     CDS             <1..289
                     /locus_tag="VGM46_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192192.1"
                     /translation="QGTVIIAKPRDIAEFTMLAKGNLILLHGHNMGRPDANTIMAATP
                     RQPMMRNRDPRFHMETSGAFLAQISFSTQRTSKETSAGNARRTSSALGGWQ"
     gene            complement(579..1148)
                     /locus_tag="VGM46_06070"
     CDS             complement(579..1148)
                     /locus_tag="VGM46_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006206890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEY4192193.1"
                     /translation="MFIQTESTPNPATLKFLPGKEVLLEGTADFRDAASAAEASPLAG
                     RLFAIDGVTGVFFGYDFITVTKDGPDWQHLKPAILGAIMEHFMSGASVMTNAGPAAET
                     SQTGEFYDAADEELVVTIKELLDTRVRPAVAQDGGDITFRGFQNGTVFLHMKGSCAGC
                     PSSTATLKHGIQNLLRHFVPEVQQVEQVS"
     gene            complement(1266..1757)
                     /locus_tag="VGM46_06075"
     CDS             complement(1266..1757)
                     /locus_tag="VGM46_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013891439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY4192194.1"
                     /translation="MVSKRLSREAGHRRKFLVIIDDTPECERAAAYAAQRMKNTNGVL
                     VLLYVIEPEDFQHWLGVEKIMRDEANANARTVLDGYATRIRERFGIEPELVVREGKPV
                     EELHKIIEEDQDIAILVLAASAGKEGPGPLVAAVVGKAAAFPIPVTVVPQNLTDEEIE
                     SLA"
     gene            complement(1864..2967)
                     /gene="trpS"
                     /locus_tag="VGM46_06080"
     CDS             complement(1864..2967)
                     /gene="trpS"
                     /locus_tag="VGM46_06080"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015314174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEY4192195.1"
                     /translation="MTATNPTPSAVNPSAFKPLVFSGVQPTGNLHLGNYLGALKKFVA
                     LQETNDCIYCVVDLHSLTAQLVHEDLAEQTRAITAAFLASGIDPKKHIVFNQSRVMQH
                     AELAWIFNCVARIGWMNRMTQFKDKAGKDRENASLGLLAYPSLMAADILVYRATHVPV
                     GDDQKQHLELTRDIAQKFNNDFSGRIADLGVGVEMQVGEETVNGYFPITEPIIGGPAA
                     RIMSLRDGSKKMSKSDPSDLSRINLTDDADTISKKIRKAKTDPAALPGEIDGLSGRPE
                     AENLVGIYAGLAETSKEAVLQEFGGQQFSAFKTALADLAVEKLAPIAGEMRRISSDRA
                     YVDAVLKDGGERAGRLAETTMKDVRNIIGLLQG"
     gene            complement(3073..4659)
                     /gene="murJ"
                     /locus_tag="VGM46_06085"
     CDS             complement(3073..4659)
                     /gene="murJ"
                     /locus_tag="VGM46_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEY4192196.1"
                     /translation="MSLVRKFATVASGTLMSRVLGFTREMLMAAALGTGPVADAFYAA
                     FQFPNTFRRLFAEGAFNTAFVPLFAKEIEAGGNDGAKRFSEEVFGVLFTVLIGITIVM
                     ELAMPLLVSTVIAPGFTRDPQKYELTVALATIMFPYLVCMSLGAMMAGMLNSLRRYFA
                     AAVAPVFLNVILIGVLYYAWRVGADGLAVGRAMAWGVLAAGIVQLAIVWAAVRHAGIS
                     VGLRRPRMTPNVKRLLWLAFPAAVTGGITQINQLIGTAIASTQAAAVSSLALADRVYQ
                     LPLGVVGIAVAIVLLPELSRALKSGNLVEAANLQNRSVEFTLFLTLPATAALLTISEP
                     IVRLLYERGAFAANGSTPTVAAILAIFGVGLPAFVLIKAFTPGYFAREDTRTPMIFAA
                     ISVAVNIVLALALFRTMGAPGIAAASSAAGWVNAVMLLGVLVRRDHWGHDPALMKRIP
                     RLVLSAAIMGAALWFGARWFAPYLASASPLLVKAATLALLCGAATILYFVLAFATGGA
                     DFGMIRRNIGRRGQASVRPE"
BASE COUNT          874 a         1449 c         1591 g          802 t
ORIGIN      
        1 ccagggcacg gtcataatcg cgaagccacg agacatcgcc gagttcacga tgctcgcgaa
       61 aggaaatctt attttgttgc atggccataa tatggggagg ccggatgcca atacaataat
      121 ggcggcgacg ccgcgccagc cgatgatgcg aaaccgcgat ccccgattcc acatggaaac
      181 cagcggtgcg tttcttgccc aaatttcatt ttcaactcag agaacctcga aggaaacctc
      241 tgccggcaat gcgcgccgga ccagcagcgc tttgggcggc tggcagtaag atcgcaaccg
      301 gtttaccgga tgccagcgcc gcgccagcgt gatggcgggg cgcccacagc gcggtcgggt
      361 tcaggtcgag cttctcgccc tcaatgcaca ttcgggattt cggggcggca tcacccggca
      421 cgacaagtca aagacaggcc ggacgcgggt aacgatcggt gttggccaga agaaaacgtc
      481 cgccggcgag aagggagcgc gtatcgcgaa gacgtccgat tccctcccgc gccacggtgc
      541 agcgccgccg gcttgaatcc tgcggtgcta agcggggctt acgaaacctg ctctacctgc
      601 tgcacttccg gcacgaaatg gcgcaggagg ttctggatgc cgtgcttgag cgtcgccgtc
      661 gaggacgggc agccggcgca ggaacccttc atgtgcagga acacggtgcc gttctggaag
      721 ccgcggaagg tgatgtcgcc gccatcctgc gccacggccg ggcgcacccg cgtgtcgagc
      781 agttccttga tggtgacgac cagttcctcg tcggccgcgt cgtagaactc gccggtctgg
      841 ctggtttcgg cggcggggcc ggcattggtc atgaccgagg cgcccgacat gaaatgttcc
      901 atgatggcgc cgaggatggc cggcttcagg tgctgccagt ccggaccgtc cttggtcacg
      961 gtgatgaaat catagccgaa gaagacgccg gtgacgccat cgatggcgaa caggcggccg
     1021 gccagcggcg aggcttcggc cgcgcttgcc gcgtcgcgga agtcggcggt gccttccagc
     1081 agcacttcct tgccaggcag gaatttcagc gtcgcggggt ttggcgtcga ttcggtctgg
     1141 atgaacatgg ccgtctcctg cccgtcatcg tcgcgggcct acagtctgga ataattctaa
     1201 ataagccttc cacaggcaag atacaagcaa aagacgcggc gccgacattg ccggccgcac
     1261 atgcctcagg ccaggctttc aatttcctcg tcggtcaggt tctgcggcac cacggtgacc
     1321 ggtatcggga aggccgccgc cttgccgacg accgccgcga ccaacggtcc cggcccctcc
     1381 ttgccggcgc tcgccgccag tacgaggatg gcgatgtcct ggtcttcctc gatgatcttg
     1441 tgcagttcct cgaccggctt gccctcacgc acaaccagtt ccggctcgat gccgaagcgc
     1501 tcgcggattc tggtggcata gccgtcgagc acggtgcgcg cattggcgtt ggcttcgtcg
     1561 cgcatgatct tctcgacgcc cagccagtgc tggaagtctt ccggctcgat gacgtaaagc
     1621 aggaccagca cgccgttggt gttcttcatg cgctgcgccg cataggcggc ggcgcgctcg
     1681 cattccggcg tgtcgtcgat gatgaccagg aacttgcggc ggtggccggc ttcccggctg
     1741 aggcgtttgg agaccatggt ctaccttatc gatggaattt cttgccggcc aatctgccac
     1801 cggccggttc cggcggcaag ccgccagaac ggatcggatg aaagtgacgg tcccggctgc
     1861 aaatcagccc tgcaacaggc cgataatatt gcgcacgtcc ttcatggtcg tctccgccag
     1921 ccggccggcg cgttcgccgc catccttcag cacggcgtcg acataggcgc ggtcggaaga
     1981 aatgcggcgc atctcgccgg cgatgggcgc cagcttctcc acggccagat cggcaagcgc
     2041 cgtcttgaac gccgagaatt gctggccgcc gaattcttgc agcaccgcct ccttggaggt
     2101 ctcggcaagg ccggcataaa tgccgacgag gttttcggct tccggacggc cggaaagccc
     2161 gtcgatttcg ccgggcaggg cggccggatc ggtcttggcc ttgcgaatct tcttcgagat
     2221 ggtgtcggca tcgtcggtca ggttgatgcg cgaaagatcg gacgggtccg atttcgacat
     2281 cttcttggag ccatcgcgca ggctcatgat gcgcgctgcc gggccgccga tgatcggctc
     2341 ggtgatcggg aaatagccgt tgaccgtctc ctcgccgacc tgcatctcga cgccgacgcc
     2401 aaggtcggcg atgcggcccg aaaagtcgtt gttgaatttc tgcgcgatgt cgcgcgtcag
     2461 ttccagatgc tgcttctggt cgtcgccgac cggcacatgg gtggcgcggt agacgagaat
     2521 gtcggcggcc atcaggcttg gataggcgag caggccgagc gaggcgtttt cgcggtcctt
     2581 gccggccttg tccttgaact gcgtcatgcg gttcatccag ccgatgcgcg ccacgcagtt
     2641 gaaaatccac gccagttcgg cgtgctgcat cacccggctc tggttgaaga cgatgtgctt
     2701 cttcggatcg atgccggaag cgaggaaggc ggcggtgatg gcgcgcgtct gctcggcaag
     2761 gtcttcatgg acaagctgcg cggtcagcga atgcaggtcg acgacgcaat agatgcagtc
     2821 gttggtttcc tgcaaggcga cgaacttctt cagcgcgccg agataattgc cgaggtggag
     2881 attgccggtg ggctgcacgc cggaaaagac gagcggcttg aaggcggatg gattgacggc
     2941 ggatggggta ggattggtgg cggtcatggt ttcctcgtgg cttgtggagg gccgtttcgc
     3001 gcgcggcgaa attcggatgg gcttatggag cacgcggcgc gcccgatcaa ggggcgccgc
     3061 gcctggcttg catcattccg gcctgaccga agcctggccg cggcggccga tattgcgcct
     3121 tatcatgccg aaatcggcgc cgccggtggc gaaggccagc acgaaataga gaatggtggc
     3181 agcgccgcac agaagcgcca gcgtcgccgc cttgaccagg aggggcgaag ccgaggcaag
     3241 gtagggcgca aaccagcgcg cgccgaacca cagcgccgcc cccatgatcg ccgccgacag
     3301 tacgaggcgc ggaatgcgtt tcatcagcgc cggatcgtgg ccccaatggt cgcgcctcac
     3361 cagcacgccc agcagcatca ccgcattgac ccagccggcg gcggacgagg cggcggcgat
     3421 gccgggcgcg cccatcgtcc ggaacagcgc cagcgccaga acgatgttca ccgccacgga
     3481 aatagcggcg aatatcatcg gcgtcctggt gtcttcgcgg gcgaaatagc ccggcgtgaa
     3541 cgccttgatc agcacgaagg cgggcaggcc gacgccgaag atcgccaata tggcggcaac
     3601 cgtcggcgtc gagccattgg cggcgaaagc accgcgctca tagagcaggc gcacgatagg
     3661 ctcggaaatg gtgagcagcg ccgccgtcgc cggcagcgtc aggaacaagg tgaactcgac
     3721 cgaacggttc tgcaaattcg ccgcctcgac cagattgccg gatttcagcg cccgcgaaag
     3781 ctccggcaga agcacgatgg cgaccgcgat gccgacgacg ccgagcggca gctggtagac
     3841 gcggtcggcc agcgccagcg acgatacggc ggcggcctgg gtggaagcga tggcggtgcc
     3901 gatcagctgg ttgatctggg taatgccgcc ggtgacggcg gcgggaaagg ccagccacaa
     3961 cagccgcttg acgttgggcg tcatgcgcgg gcggcgaagg ccgaccgaaa tgccggcgtg
     4021 gcgcacggcg gcccagacga tggccagctg cacgatgccg gcggccagca cgccccaggc
     4081 catggcgcgg ccgacggcca ggccatcggc gccgacgcgc catgcatagt acagcacgcc
     4141 gatcaggatg acgttgagga agacgggggc gacggcggcg gcgaaatagc ggcgcagcga
     4201 attcagcatg ccggccatca tcgcgccgag cgacatgcag accagatagg ggaacatgat
     4261 ggtggccagc gccaccgtca gctcgtattt ctgcggatcg cgggtgaagc ccggcgcgat
     4321 gacggtgctg accagcagcg gcatcgccag ttccatcacg atggtgatgc cgatcagcac
     4381 cgtgaacagg acgccgaaca cctcctcgga aaaccgcttg gcgccgtcat tgccgccggc
     4441 ctcgatctcc ttggcgaaca gcggcacgaa ggcggtgttg aaggcgcctt cggcaaacag
     4501 ccggcggaag gtgttgggaa actggaaggc ggcgtagaag gcgtccgcca ccggtccggt
     4561 gccaagggcc gccgccatca gcatttcgcg ggtgaagccg agcacgcggc tcatcagcgt
     4621 gccggaggcg acggtggcga attttcttac gaggctcatg ctgcgtcctt cgaggctcgc
     4681 ggagactgtc ttctggcttg ccgaagaaaa gatgcg
//
LOCUS       DASYCS010000188         3929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_768707,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000188 DASYCS010000000
VERSION     DASYCS010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3929
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768707"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VGM46_06090"
     CDS             <1..471
                     /locus_tag="VGM46_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY4192197.1"
                     /translation="GWRMGWSIWPNGEKGGHLYDKVRKLAVNCWSCVNAPSQFAGIAA
                     IDGPQDDVTRMMQAFDDRRQMVVEGLNALPGISCVTPKGAFYAFPNISGTGWKAKKLA
                     TALLEEAGVALIGGPDFGILGEGYIRLSYANSQENILHALERIEAFLRKPAAAN"
     gene            complement(478..1233)
                     /locus_tag="VGM46_06095"
     CDS             complement(478..1233)
                     /locus_tag="VGM46_06095"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003505980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY4192198.1"
                     /translation="MANGKAAIVTGAGTGIGKAVATALLKHGWNTVFCGRRKDMLDRA
                     IAEAGPSDAKALGVSCDITRPEEVEALFRQTVENFGRVDLLFNNAGMGYKSASIDEIP
                     VAVWNDVVAVNLTGSFLCARAAFAAMRHQQPMGGRIINNGSVSAYAPRPGSAPYTATK
                     HAITGLTKTLALDGRPYDIACGQIDIGNALTEMARPMTVGVPQANGSTAAEPVMDVER
                     VADAVLHMASLPLDANILFMTVMATKMPFVGRG"
     gene            complement(1462..2199)
                     /locus_tag="VGM46_06100"
     CDS             complement(1462..2199)
                     /locus_tag="VGM46_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HEY4192199.1"
                     /translation="MFDPKKLLDDLLGSQIPGTSGTVRDKAGQAVQYAKDNPLAAGAL
                     AAVLFGTGTGRQVTGAAVKLGGLAAIGGLAYKAYQNYQNGRTPVGEAEAGEPELLPPP
                     SDTGFHPSQAPQGETEFALTLVRAMIAAARADGQIDDDERRKIGAKLGDVGADSEAEA
                     FIKSELGAPLDIEALVASAQSEEQKVELYTASRLAIDADTRAERGYLDLLAGRLGLPD
                     ALVEHIEATVSSATVPVAAGTTPNPRW"
     gene            complement(2323..3243)
                     /locus_tag="VGM46_06105"
     CDS             complement(2323..3243)
                     /locus_tag="VGM46_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006338888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEY4192200.1"
                     /translation="MNISTRPLRVAALYRFCRLDAFEALRAPLAEFCGARGIRGTLLL
                     ASEGINGTVAGSDEAIDELVAYLRAIPGMSGLDVKFSSAATMPFHRMKVRLKREIVTM
                     GVDAVDPEGSAGTYVAPADWNVLIADPGTVVIDTRNRYEVALGTFQGAVDPDTASFRE
                     FPEWVEKHRAELDGKKIAMFCTGGIRCEKATAYVKSLGVEQVFHLKGGILKYLEEVPA
                     GQSLWQGECFVFDERVSVSHGLIEGDAELCRACRLPLTPNDRLSPRYEAGVSCPHCFE
                     ARSPEDRQRYAERQRQVELACARGEEHIGR"
     gene            3414..>3929
                     /locus_tag="VGM46_06110"
     CDS             3414..>3929
                     /locus_tag="VGM46_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019170995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramidase domain-containing protein"
                     /protein_id="HEY4192201.1"
                     /translation="MFTQDVISEITAAAQGLGIEPASLLAVAEVESGGTAFADIDGRH
                     EPLIHFEAHYFDRRLSDGNRALARHKGLAAPVAGAIANPASQAARWQMLEQAAAIDAK
                     AAYESVSWGLGQVMGAHWAWLGYASVDALVAEARSGVAGQARLMALYIDKAGLAEALR
                     NHDWIAFAKGYN"
BASE COUNT          718 a         1284 c         1208 g          719 t
ORIGIN      
        1 ggctggcgca tgggctggtc gatctggccg aacggcgaaa agggcggcca tctctacgac
       61 aaggtgcgca agctggcggt caattgctgg tcctgcgtca acgcgccgtc gcaatttgcc
      121 ggcatcgccg ccatcgacgg cccgcaggac gacgtgaccc gaatgatgca agccttcgac
      181 gaccgccgcc aaatggtggt cgagggcctg aatgccctgc ccggaatttc ctgcgtaacg
      241 cccaaggggg cgttctatgc ctttcccaac atttcaggca ccggctggaa ggcgaagaaa
      301 cttgccaccg ccttgcttga ggaagccggc gtggcgctga ttggcgggcc ggatttcggc
      361 atattgggcg aaggctatat ccgcctttcc tacgccaatt cgcaagaaaa catcctgcac
      421 gcgctggaac gcatcgaggc gttcctgcgc aaaccggcgg cggcgaacta gccttgctca
      481 gccacgcccg acgaacggca tcttcgtcgc catcaccgtc atgaacagga tgtttgcatc
      541 gagcggcagg ctggccatgt gcaaaaccgc atcggccacg cgctcgacgt ccatgaccgg
      601 ctcggcggcc gtcgatccgt tggcttgcgg cacgccaacc gtcatcggcc gcgccatttc
      661 ggtcaacgcg ttgccgatat cgatttggcc gcaggcaatg tcgtagggcc gcccatcgag
      721 cgcaagcgtc ttggtcaggc cggtaatggc gtgcttcgtc gccgtgtagg gtgcggagcc
      781 ggggcgcggc gcataagccg acaccgagcc gttattgatg atgcgcccgc ccatcggctg
      841 ctggtggcgc atcgccgcga acgcggcccg cgcgcatagg aacgagccgg tgaggttgac
      901 ggccaccaca tcgttccaga cggcgacggg aatttcgtct atcgacgccg atttgtagcc
      961 catgcctgca ttgttgaaga gcaggtccac ccggccgaaa ttctccaccg tctgcctgaa
     1021 caacgcctcg acctcttccg gcctggtgat gtcgcagctg acccccagcg ccttcgcgtc
     1081 ggaaggcccc gcctccgcaa tcgccctgtc gagcatatcc tttcggcggc cgcagaatac
     1141 cgtgttccag ccgtgcttca gcaacgcggt ggcgacggcc ttgcctatcc ccgtgccggc
     1201 cccggtgacg attgccgctt tgccgtttgc catgttttcc ctcgctggat gaccatggca
     1261 tcgcaaatgc cattgcggct ggcaagcatc gccatgcgcc aaataaacag gcatcgccat
     1321 gcgccaaata aaaagggcgg ccattggcga ccgccccaat ctggacctgg cttgggagga
     1381 ggaagccgat ccgactaaga caaatgatgc gcttatccgg ttaacgaaaa cctaacatga
     1441 agtctccgcc accgtaggcg gctaccagcg cggattgggc gtcgtgcctg cggccaccgg
     1501 cacggttgcc gacgaaacgg tcgcctcgat gtgctcgaca agagcgtccg gcaagccgag
     1561 cctgccggca agcaggtcga gatagccgcg ctcggcgcgc gtgtcggcgt cgatggcaag
     1621 gcgcgaggcc gtgtaaagct cgaccttctg ctcctcgctc tgtgccgacg cgacaagcgc
     1681 ctcgatatcg agcggcgctc ccagttccga cttgatgaag gcttccgcct cggaatccgc
     1741 gccgacgtcg ccaagcttgg cgccgatctt gcggcgttcg tcgtcatcga tctggccgtc
     1801 ggctctcgcc gccgcgatca ttgcccgcac cagcgtcagc gcgaattcgg tctcgccctg
     1861 cggcgcttgc gacggatgaa agccggtgtc ggagggcggc ggcaacagtt cgggctcgcc
     1921 ggcttcagcc tcgccaacgg gcgtcctgcc gttctggtag ttctggtagg ccttgtaggc
     1981 gaggccgcca atcgccgcca ggccgcccag cttcaccgcc gcgccggtga cctgcctgcc
     2041 ggttccggtc ccgaacagca ccgccgccag cgcgccggca gccagtggat tgtccttggc
     2101 gtattgcacc gcctgcccag ccttgtcgcg caccgtcccg ctcgttccgg ggatttgcga
     2161 gccgagcaga tcgtcgagca atttcttggg atcgaacatc aacacctcca gatagccacg
     2221 cgggccggtt tcgggtgacg aaaaagtaag gccgcaacgc tgacattaca atgacaggga
     2281 tcgatttgcc tgcccttgcc gcttccttga aatgcgtcgg cgctatcggc cgatatgttc
     2341 ctcgccgcgc gcgcaagcca gttccacctg gcgctggcgt tcggcatagc gctgacgatc
     2401 ctcgggcgag cgcgcctcga aacaatgagg acaggaaaca cccgcctcat atctcggcga
     2461 aagccggtcg ttcggcgtca gtggcagccg gcaggcacgg caaagttcgg catcgccctc
     2521 gataagcccg tgcgacaccg aaacccgctc gtcgaacacg aagcactcgc cctgccacaa
     2581 gctttgcccg gccggtactt cctcaagata tttcaagatg ccgcccttga ggtggaacac
     2641 ctgctcgacc ccaagcgact tcacataggc cgttgccttt tcacagcgga tgccgccggt
     2701 gcagaacatg gcgatcttct tgccgtccag ttcggcccgg tgcttctcta cccattcggg
     2761 aaactccctg aagcttgccg tgtccggatc gacggccccc tgaaacgtgc cgagcgccac
     2821 ttcatagcgg ttgcgcgtat cgatgacgac ggtgccggga tcggcgatca gcacgttcca
     2881 atcggccggc gcgacatagg tgccggcgct tccttcggga tcgaccgcat cgacgcccat
     2941 cgtgacgatc tcgcgtttca atcgaacctt catgcgatga aacggcatgg tggcggcgct
     3001 gctgaatttc acgtcgaggc cggacattcc cggaatggcg cgaaggtaag cgacgagttc
     3061 gtcaatcgct tcgtcgctgc cggcgaccgt gccgttgatg ccctccgaag cgagcagaag
     3121 cgtgcccctg atgccgcgcg ccccgcaaaa ttccgccagc ggcgcgcgca acgcctcgaa
     3181 tgcgtcaagc cggcaaaatc gatagagcgc agcgacgcgc aaagggcgtg tggatatatt
     3241 catggcaaaa gcggttaaac gctgcgctta gccgtttcaa ggcaaagccg attcatgcgt
     3301 gcaatcaatg tgttggcgcg ccccctgcca gcccttggaa taattgtttg cgcatttttg
     3361 gtccacatgc cggcaattgc ggcgacactt tctccaaaca ggagataagc gccatgttca
     3421 cgcaagacgt catttcggaa atcacggccg ccgctcaggg tctcggcatc gagccggcat
     3481 cgctgctggc agttgctgaa gtggagagcg ggggcacagc cttcgccgat atcgatggcc
     3541 ggcacgagcc attgatccac ttcgaggcgc attatttcga ccggcggctg agcgatggca
     3601 accgcgcgct cgcgcgccac aaaggtcttg ccgcgccggt ggcgggcgcc atcgccaacc
     3661 cggcaagtca ggctgcgcgc tggcagatgc tggagcaggc ggctgccatc gacgccaagg
     3721 ccgcttacga atcagtctcc tggggcctcg gacaggtcat gggcgcgcat tgggcatggc
     3781 ttggctatgc cagcgtcgac gcgctggtcg cggaggcccg gtcgggcgtg gccgggcagg
     3841 cgcggctgat ggcgctctac atcgacaagg ctggcctggc cgaagccctg cgcaatcacg
     3901 actggatagc cttcgcaaaa ggttataac
//
LOCUS       DASYCS010000189         3226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3812507,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000189 DASYCS010000000
VERSION     DASYCS010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3812507"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /gene="cbiT"
                     /locus_tag="VGM46_06115"
     CDS             <1..459
                     /gene="cbiT"
                     /locus_tag="VGM46_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6Y C5,15-methyltransferase
                     (decarboxylating) subunit CbiT"
                     /protein_id="HEY4192202.1"
                     /translation="GELLWDIGAGSGSISIEWMLAHPSLRAIAIEADPERATRIRRNA
                     ISCGVPGLAIVEGPAPQALSGLEQPGAIFIGGGGSDAGVVEAAIQALPAGGRLVANGV
                     TLEMEAVLLASHAALGGELTRLSVSRASPVGSMLAWRPAMPVTQWNWTKP"
     gene            438..842
                     /locus_tag="VGM46_06120"
     CDS             438..842
                     /locus_tag="VGM46_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HEY4192203.1"
                     /translation="MELDKTMIVAGIGCKKGVAAHDIRAALDAALAAHGLSSASLSAL
                     ATAKFKHAETAITETAHALRLPVLLVDNAALTAVSLRTLTHSHHSQSVTGTPSLSETA
                     ALAAAGQHARLLGPRVVVGGVTCAIAIDGGRS"
     gene            839..1603
                     /gene="cobM"
                     /locus_tag="VGM46_06125"
     CDS             839..1603
                     /gene="cobM"
                     /locus_tag="VGM46_06125"
                     /EC_number="2.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046026 - precorrin-4
                     C11-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-4 C(11)-methyltransferase"
                     /protein_id="HEY4192204.1"
                     /translation="MTVHFIGAGPGAPDLITMRGAKLLADCPVCLYAGSIVSPELLQY
                     CAPATRKIDTAPMSLDEIEAEYLAAHRAGHDVARLHSGDLSVWSAVAEQIRRLEKHGI
                     AYTLTPGVPSFAAAASALRRELTIPEVAQSLVLTRVSGRASKMPPGETLAAFGRTGAT
                     LAIHLAIHAIDHVVSELAPLYGADCPVAIVFRASWPDERVLRGTLATIEAQLAADPIE
                     RTAIIFVGRSLAAQDFTESSLYDAYYQRRFRGRDGL"
     gene            1600..2415
                     /gene="cobA"
                     /locus_tag="VGM46_06130"
     CDS             1600..2415
                     /gene="cobA"
                     /locus_tag="VGM46_06130"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015317325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEY4192205.1"
                     /translation="MTDKDDNGGNAGLIRALSRFDSPLRTLEAGHVWLAGAGPGDPGL
                     LTLDVLSALAQADALVHDALVSPEIIAVAEQAEKFYVGKRGGRLSIPQADINALLVRL
                     ASEGRKVVRLKGGHPYVFGRGGEEALALVENNIPYRVLPGVTSAFGGLTSAGIPATMR
                     GVNGAIILATGFAAVSDDRPDWTALARTGQPIIIYMGLTHMAATVADLRAGGLGEDTP
                     AALIENATLPTERTVVATLGTLVDAAAREHITSPALIVVGGIVAMRKQLAGRA"
     gene            2412..>3226
                     /locus_tag="VGM46_06135"
     CDS             2412..>3226
                     /locus_tag="VGM46_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013894794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HEY4192206.1"
                     /translation="MTAKAIIVGAPRSGSGKTSVTIGILRALSRRGIRVRGAKSGPDY
                     IDPGFHAAATGLPGVNLDSWAMSPALLNGLAGQAADDAEFVVIESAMGLFDGIPSTPG
                     RTGAAADLAALYGTPVLLVLDVSGQSTTVAAVAKGFATYRPDVHIAGVVLNRLGSERH
                     RALCTDAVEALGLPVLGAVMRDPTLSMPERHLGLVQASEHTNLMAFIDRMADMVENSI
                     DLDAVMRHAVPLVPAEGGFDQHLPPPGRRIALAQDAAFTFLYPHVAAHWRRNGA"
BASE COUNT          494 a         1140 c         1019 g          573 t
ORIGIN      
        1 ggcgaactgc tgtgggacat aggcgcggga tccggctcca tctccatcga atggatgctg
       61 gcccacccct cgttgcgcgc cattgccatc gaggccgacc ctgaacgggc cacccgcatc
      121 cgccgcaatg ccatatcctg cggcgtcccc ggtctggcca ttgtcgaagg ccctgccccg
      181 caagcactgt ccgggctgga gcagcccggc gcgatcttca tcggcggcgg cggcagcgat
      241 gccggcgtgg tggaggccgc catccaggcg ctgcccgccg gcgggcgctt ggtcgccaac
      301 ggcgtgacgc ttgagatgga ggcggtgctt ctggccagcc atgcagcgct tggcggcgag
      361 cttacccgcc tctccgtttc ccgcgcttcc ccggttggtt cgatgctggc gtggcggccg
      421 gccatgccgg ttacgcaatg gaactggaca aaaccatgat cgtcgcgggc atcggctgca
      481 agaaaggcgt tgccgcgcac gatatacgcg ccgcgctcga tgcggcgctg gcagcgcatg
      541 ggctttcatc ggcatcgctg tcggctctcg ccaccgcgaa gttcaaacat gccgaaacag
      601 ccatcaccga aaccgcccac gcgctgcggc ttccggtttt gcttgtcgac aatgccgccc
      661 tcaccgccgt ttccctgcgc acgctgaccc attcccacca ttcgcagtcg gtgaccggaa
      721 caccctcgct ttccgaaacc gcggcccttg ccgccgccgg ccagcatgcg cgcctgcttg
      781 ggccgcgtgt cgtggtcggc ggcgtcacct gcgccatagc aatcgacgga ggccggtcat
      841 gacggttcat ttcatcggcg cggggccggg tgcgccggac ctgatcacca tgcgcggcgc
      901 aaagcttctg gccgattgcc cggtctgcct ttatgccggc tccatcgtct cgccggaact
      961 gcttcagtat tgcgcgcccg cgactagaaa gatcgatacc gcgcccatgt cgctggacga
     1021 gatcgaagcc gaatatctcg ccgcccatcg ggccggccac gatgtcgccc ggctgcactc
     1081 cggcgacctg tcggtgtgga gcgcggtagc cgagcagatt cgccggctgg aaaaacacgg
     1141 catcgcctat acgctgacgc cgggtgtccc ttccttcgcc gccgccgcct cggcgcttcg
     1201 gcgcgaactg acgatccccg aggtcgcaca gagcctggtg ctgacgcgtg tttccggccg
     1261 tgcctcgaaa atgccgccgg gcgagacgct tgccgctttc ggccgcacgg gcgccacgtt
     1321 ggccatccac ctcgccatcc acgccatcga ccatgtggtc agcgaacttg cgccactcta
     1381 tggcgccgat tgcccggttg cgatcgtctt tcgcgcgtca tggccggacg agcgcgtctt
     1441 gcgcggcacg ctcgccacca tcgaagcgca gctggccgcc gatccgatcg agcgaaccgc
     1501 catcattttc gtcggacgct cgctcgccgc gcaggatttc acggaaagct cgctctacga
     1561 cgcctattat cagcgccgct ttcgcgggcg ggacggacta tgaccgacaa agacgataat
     1621 ggcggcaacg ccgggctgat ccgcgctctt tcgcgcttcg attctccgct gcgcacactg
     1681 gaagccggcc atgtctggct ggccggtgcc gggccgggcg atccaggcct gttgactctg
     1741 gacgtattga gcgcgttggc tcaagccgat gcgctggtgc atgacgcgtt ggtgtcgccc
     1801 gaaatcatcg ccgtcgccga acaggccgag aaattctatg tcggcaagcg cggcggcagg
     1861 ctgtcgatcc cgcaggcgga catcaatgca ctgctggtcc ggctggcgag tgaaggccgc
     1921 aaagtggtgc ggctcaaggg cggccaccct tatgtgtttg ggcgcggcgg cgaggaagcg
     1981 ttggcgctgg tcgaaaataa tatcccctac cgggtattgc cgggcgtcac ctcggccttt
     2041 ggcggcctga cctcggcggg cattcccgcc accatgcgcg gcgtcaacgg cgcgatcatt
     2101 cttgcaaccg gcttcgccgc cgtttccgat gacaggccgg actggaccgc ccttgcgcgc
     2161 accggccaac ccatcatcat ttatatgggg ctgacgcata tggccgctac ggtggccgat
     2221 ctgcgcgccg gcgggctggg cgaagacacg ccggcagccc tgatcgaaaa cgccaccttg
     2281 ccaaccgaac gcaccgtcgt cgccaccctt ggcacgctgg tagatgccgc cgcgagagag
     2341 cacatcactt ctccggccct gatcgtcgtt ggcggcatcg tggcgatgcg caaacagctg
     2401 gcaggccggg catgaccgcc aaggccatca ttgtcggcgc accgcgctcc ggctccggca
     2461 agaccagcgt gaccatcggt atcctgcgcg ccctgtcgcg gcgcggcatt cgcgtgcgcg
     2521 gggcgaaatc ggggccggac tatatcgatc ccggcttcca tgcggcggca accggccttc
     2581 caggcgtcaa tctcgacagt tgggcaatgt cgccggcgct gcttaacggc cttgccggcc
     2641 aagccgccga cgacgccgaa ttcgtcgtca tcgaaagcgc catgggtcta ttcgacggca
     2701 tcccctcgac gccgggccgc acgggcgccg ccgccgatct ggctgcgctc tacggcacgc
     2761 ccgtattgct ggtgctggac gtctccggcc aatccaccac cgtggctgcc gtcgccaagg
     2821 gcttcgccac ctaccgtccc gatgtgcata tcgcaggcgt cgtgctcaac cgcctgggca
     2881 gcgagcgcca ccgcgcgctc tgcaccgacg cggtcgaggc gctcggtctg cccgtgcttg
     2941 gcgcggtgat gcgtgaccct acgctttcca tgccggagcg gcatctgggc cttgtccagg
     3001 caagcgagca cacgaacctg atggccttca tcgatcggat ggccgacatg gtcgaaaatt
     3061 ccatcgacct cgatgccgta atgcggcacg ccgttccgct ggttccggcc gaaggcggtt
     3121 tcgaccagca tttgccgccg cccggccggc gcatcgccct ggcgcaggac gccgccttca
     3181 cctttctcta tccgcatgtc gccgcccact ggcgccgcaa cggggc
//
LOCUS       DASYCS010000190         2522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1641468,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000190 DASYCS010000000
VERSION     DASYCS010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1641468"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..858
                     /locus_tag="VGM46_06140"
     CDS             175..858
                     /locus_tag="VGM46_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017995455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY4192207.1"
                     /translation="MTSRTILIVDDDEDLRATLVEQLALYEEFDVLQEANAAKGVAAA
                     RAGLIDLLIMDVGLPDMDGREAVKILRKGGYKAPIIMLTGHDTDSDTILGLEAGANDY
                     VTKPFRFAVLLARVRAQLRQHEQSEDATFAVGPYTFKPSQKLLIDQRGGKVRLTEKEA
                     SIIKYLYRAEQKVVTRDVLLEEVWGYNSGVTTHTLETHVYRLRQKIERDPSNAEILVT
                     ESGGYKLVP"
     gene            999..1457
                     /locus_tag="VGM46_06145"
     CDS             999..1457
                     /locus_tag="VGM46_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY4192208.1"
                     /translation="MALDDDIRILSAVGLFEGFTQEQLRLLAFGAETTLLAADRKLYR
                     EDDEADSAYVVVSGRIVLYREGDGSERLAIGHVGPGAIMGELALIADTRRLTSASAEI
                     DSEVLRLSRRMFRRILEEYPDAAVQIQRRITAQLQEFLQKIENLAPRFAG"
     gene            1459..1959
                     /locus_tag="VGM46_06150"
     CDS             1459..1959
                     /locus_tag="VGM46_06150"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY4192209.1"
                     /translation="MNLTLARATEADLPFIMATERLEGYAALVGNWEEQRHREALADG
                     SHAYFIGLDGPHPVGFATLRHWNSADGVTLVRRVAVATPGRGQGRKLLAGVADRVFEE
                     TAAYRLHIGLFPENLRARRAYEAAGFTPEGIARAAVFFGGVHRDELVMSLLRPEWQAR
                     RKPSAG"
     gene            complement(1965..>2522)
                     /locus_tag="VGM46_06155"
     CDS             complement(1965..>2522)
                     /locus_tag="VGM46_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEY4192210.1"
                     /translation="FCEIEESRHLSVRVQAGGKALLLHNFYVPAGGDEPDPAINRKFR
                     HKLDFVAEMNSIRAEHDEASGSVLVGDLNIAPLEHDVWSHKQLLNVVSHTPVETVNFE
                     AMREGGGWVDLMRLNIPADQKLYTWWSYRAQDWEASNRGRRLDHVWSSPNLTPLFRGY
                     EILKAARGWERPSDHVPVIARFDLD"
BASE COUNT          507 a          773 c          774 g          468 t
ORIGIN      
        1 cggtcagaac gcgaagacct gtcggtaaaa ttgcgccggc tgcattgcag gagcgttttt
       61 tcttgtatga tcgtgccacg gaacaaatca ctgtgatggg ctgttttgtt catcagcttc
      121 cgcataaacg ggcagcggtc aattgaaatt catttcaaaa caacggattg atccatgact
      181 tcacgcacca ttctgattgt cgatgacgat gaagacctgc gcgcgacgct tgtagagcaa
      241 ttggccctct atgaagaatt cgacgtgctt caggaggcga atgccgccaa gggtgtcgcg
      301 gcggcgcgcg ccgggctgat cgatctgctg atcatggatg tcggcttgcc cgacatggac
      361 ggccgcgagg ccgtgaaaat cctgcgcaag ggtggctaca aggcccccat catcatgctg
      421 accgggcacg ataccgattc cgacaccatt ctcggactgg aagcgggcgc caacgactat
      481 gtgaccaagc cgtttcgctt cgccgtgctt ctggcccgcg tgcgcgcgca attgcgccag
      541 cacgaacaaa gcgaggacgc cactttcgcg gttggcccct ataccttcaa gcccagccag
      601 aaactgctga tcgaccagcg cggcggcaag gtgcggctga ccgaaaagga agcctcgatc
      661 atcaaatatc tctaccgtgc cgaacagaag gtggtgacgc gcgacgtgct tctggaagag
      721 gtctggggtt acaattcagg cgtcaccacg catacgctgg aaacccacgt ctaccggctg
      781 cgccagaaga tcgagcgcga cccttccaat gccgagattc ttgtgacgga aagcggcggt
      841 tacaagctgg ttccgtgaag gaataagccg tatgtaagct gactgaacat attgactacc
      901 ggtacgcggt tcggggcggg gccgcaggga agtaaagcat gatccggctt cgggaccatg
      961 gcacggaaag gggaacggcc tgacggttcg gggacataat ggcgctggat gacgacattc
     1021 gcatcctgtc ggccgtgggg cttttcgaag gcttcacgca ggaacagttg cgcctgctgg
     1081 ccttcggcgc cgaaaccacc ctgcttgccg ccgacaggaa gctttaccgc gaggacgacg
     1141 aggcggattc ggcctatgtc gtggtcagcg gccgcatcgt tctctaccgc gaaggcgacg
     1201 gcagcgagcg cttggccatc ggccatgtcg ggccgggcgc catcatgggc gaactggcgc
     1261 tgatcgccga taccagacgg ctgaccagcg catcggcgga aatcgattcg gaagtgctgc
     1321 gcctcagccg ccgcatgttc cggcgcatcc tggaggaata tccggatgcc gcggtgcaga
     1381 ttcagcggcg cattaccgcg caattgcagg aattcctcca gaagatcgaa aatctcgccc
     1441 cccgcttcgc cggctagcat gaacctgacg cttgcccgcg ccaccgaagc cgacctgccc
     1501 ttcatcatgg cgaccgagcg gcttgaaggc tatgcagcgc ttgtcgggaa ctgggaagaa
     1561 cagcgccacc gcgaggcgct tgccgatggc agccatgcct atttcatcgg cctcgatggc
     1621 ccacaccccg tcggcttcgc cacgctgcgc cattggaatt cggcggatgg cgtcaccctg
     1681 gtgcgccgcg tggcggtagc gacgccgggc cggggccagg ggcgaaagct gcttgccggc
     1741 gtcgccgacc gcgtctttga agaaaccgcc gcctaccgcc tccatatcgg ccttttcccc
     1801 gaaaaccttc gtgcccggcg cgcctatgaa gcggcgggct ttacgcctga aggcattgcg
     1861 cgcgcagcgg tgttcttcgg cggcgtccac cgcgacgaac tggtgatgtc gctgctgcgc
     1921 cccgaatggc aggcgcggcg gaaaccatcc gccggctgac gatttcaatc aaggtcgaag
     1981 cgggcgatca ccggcacatg gtccgaaggc cgctcccagc cgcgcgccgc tttcaggatt
     2041 tcatagccgc gaaacagcgg ggtcaaatta ggcgaggacc agacatggtc gagacgccgg
     2101 ccgcggttgg aagcttccca gtcctgcgcg cgatagctcc accatgtata gagcttctgg
     2161 tccgccggaa tgttcagccg catcagatcg acccagccgc cgccttcgcg catcgcctcg
     2221 aaattcaccg tctcgaccgg cgtgtggctg acgacgttca gcaattgctt gtgcgaccag
     2281 acatcgtgtt ccagcggcgc gatgttgagg tcgccgacaa gaaccgaacc ggaagcctcg
     2341 tcatgctcgg cgcggatcga attcatttcg gcgacgaaat ccagcttgtg cctgaattta
     2401 cggttgatcg ccggatcagg ctcgtcgccg ccggccggca catagaaatt atgcaggagc
     2461 agggccttgc cgccggcctg aacccgcacc gaaagatgcc ggctttcctc gatttcgcaa
     2521 aa
//
LOCUS       DASYCS010000191         8746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4538844,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000191 DASYCS010000000
VERSION     DASYCS010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 8746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8746
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4538844"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="VGM46_06160"
     CDS             <1..509
                     /locus_tag="VGM46_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019170348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY4192211.1"
                     /translation="GKPFAALDALVDWLPANLPQDDAQLSIAHGDFRLGNLLFHPTRP
                     EVTGILDWELSTLGHPLADLGFCCMPWHTSPSEYGGILGLDWQALGIPLQARFADHYF
                     AHARPTAPLLPFHIVFALFRFAVIFVGIADRARAGNAAGADAQNVAPLAGRFAERALE
                     IIKGKHIL"
     gene            complement(615..1355)
                     /locus_tag="VGM46_06165"
     CDS             complement(615..1355)
                     /locus_tag="VGM46_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019998163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEY4192212.1"
                     /translation="MPTHTLPRAALAPDHNITRKTMKGVLHELVDNIVSGTWPQGSFL
                     PAEEVLLNHFGVSRPTLREGLQHLVAQGLIRSRPRAGTVVLPQSSWNLLDTMVLEAAL
                     RHIRDREFYDNLVDARMLIEPEAAARAASCASPRALADIGAAFADMVESEGRDTESWS
                     IADLAFHTAVIEASGNWVLRQFIVAMRAALMASFRMTNRASQSHEGAIAMHRKVHDAI
                     QRGEPDQARAAMQALIGLAREDMKRSLG"
     gene            1507..2475
                     /locus_tag="VGM46_06170"
     CDS             1507..2475
                     /locus_tag="VGM46_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY4192213.1"
                     /translation="MTIWKTVALGFMAALIGSSAVRADDIKIGAIYMDAQGYYAGVRK
                     GIQDAAKEADKKLQIIETNAQGDVAKESSFIDTLIASGVQAVITSPVSADGSYRAIRR
                     AHDAGIPVICYNTCLSDGDQKAYVSAYAVGDPFEFGNKLGKQAADYFVKAGIKEPKIA
                     VLNCEFVEVCVTRREGFEAALKEKVPGYKIVDNQEGAVLDKAVSVASTILTANPELDA
                     IFGEAGGATLGAARAIRAQNRVGHVVVFGGDMTAEIATELGQFDVIKAVVDISGQGMG
                     RLAFAQALNVIAGKPPQDIKVPYNIDLYASSEDGKGWLEAHKDGIP"
     gene            2552..4075
                     /locus_tag="VGM46_06175"
     CDS             2552..4075
                     /locus_tag="VGM46_06175"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEY4192214.1"
                     /translation="MQQAQSAFEARPVVASLQGCSKRYPGVVALADADFEMRAGEVRA
                     MLGKNGAGKSTLIRLLTGAETPDTGTVTIGGEELAGSDSTRAAEAFAKGVRVVYQELS
                     LVTELSIAENLFLGRWPRRAGVIRHGQMEQEARAALDSLGVDLAPGRIVSSLSPAERQ
                     LVEIARVMIGSPRLVILDEPTSSLAEAEARKVLAAVMRIAAAGISVIYVSHRMNEIRQ
                     IADTVTIMRDGRIIETVPVEGAQTGDIVKLMLGAEMDANEHIQPGLRGAPVLELREVS
                     LAPKLDRISFTLHAGEVLGIAGLLGSGRTELLQLIMGLRNRSAGEVLVGGRRVDGASY
                     RGMVRRGFGLTPESRKEEGIIPMLGVDENTVITDFSAVSRNRILSATRIAEATRRVIE
                     RLQIKTARTDTPIGTLSGGNQQKVVIGRWVHAGSRVLLLDEPTRGVDVEAKAQIYRII
                     RELAAEGCAVIFVSSEIEELPLVCDRVLVLRDRRIAEEIAAPGIDQDRLLAACIAAA"
     gene            4091..5068
                     /locus_tag="VGM46_06180"
     CDS             4091..5068
                     /locus_tag="VGM46_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016558108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4192215.1"
                     /translation="MSVTNAENRRFGLAGYMNELSLLSAIVILYLVFGLTANGFLSFP
                     NQINILRDAATIGIAAWAATLIIICGEIDVSTGPMVAFISVILAYLLQWGTPTVLAFA
                     LAIVLGGLLGALAGALRAWFDVPSFVATLGLWSALRGLALFMTDALPVSIGRNDIVDL
                     LDRPFLGLPPAVILMLALFVLFLFISRKTAFGRSVYAVGGNPHAALLAGINVARIRIL
                     VFAIGGAMAALSGILLLSRLGSGNATAASGLEFDVIAAVVVGGTALSGGRGSMVGTLL
                     GVLVITLIGNGLILLGINPFIQQVVRGVIIVVAVLANIQAIKRSRAQRS"
     gene            5070..5945
                     /locus_tag="VGM46_06185"
     CDS             5070..5945
                     /locus_tag="VGM46_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018897699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HEY4192216.1"
                     /translation="MTVIALRSGDMRARITTHGGSILRLDQGAVPLLRPADDDALPID
                     SACYPLVPFGNRVRGNRFTFQDKAYTLAPNTDWDAHYLHGEGWRSDWRVVNRQEDRLV
                     LSHEHDGSSMPWIYSAEQRFNLRPDMIELEISVTNRGNEAMPFGLGWHPYFPMTPQTT
                     LHTRTGRMWTEEAGWLPGKAVDLPQDLDFSAPKGLPDHWVNNGFEDWSGEARIDWPER
                     GRALRVEAAPLFRVMFLFVSSTSFDPSYRRDFFALEPMSHLADGHNMPDLGGLAPLAP
                     GESLSGSLRLKVEHS"
     gene            5946..7565
                     /locus_tag="VGM46_06190"
     CDS             5946..7565
                     /locus_tag="VGM46_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEY4192217.1"
                     /translation="MASRSYDYILVGGGSSACAAAARLLSSGASVLMLERGPGRSNPI
                     MDFPAGYMKFLARDTYLVMHDTVPQPQLDGRAPIVPQGRVLGGGGAVNAMVYMRGQRE
                     DYAEWNRAIGGDGKGAWDWDDLLPHYLRQEDNDHLGGPLHGVGGPLKVSHLGHTSPMT
                     RAWLKTMQQMGIPYTPDFNNGDPRGVGLMQHTIDWEKRRRSSPVEAFLAPWLKNPLLD
                     LHCQATVGSILWEGNRAIGVSWREGGKAHEARAGEVLMAAGAYQTPKLLMLSGVGPEE
                     ELAAHGIATRIALPGVGANLQDHYECPVVATTNGAYGYYGQDKGLKMLLAGLQYLLFR
                     SGPVTTTGVESCAFLDPTGQGGRPTIQMFCVPTVYLDRDVMGADPGHGVTINSLLLQP
                     KSRGHVKLRSADPDALPLVDLQIFAERADLDLTVQGFRFARSVLERSPLRDLVKAEIF
                     PGRDVVSDEAIAAHCRRTVKTGYHPVGTCRMGGDDDAMAVLDASLKVRGAQGLRVIDA
                     SMMPNIVSGNTNAAVMAVADKAADLILGTTP"
     gene            7576..8727
                     /gene="dgoD"
                     /locus_tag="VGM46_06195"
     CDS             7576..8727
                     /gene="dgoD"
                     /locus_tag="VGM46_06195"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018497475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HEY4192218.1"
                     /translation="MKITGLKTFIVPPRWLFLKIETDEGVCGWGEPVVEGRAHTVETA
                     VRELADMLVGQDPSRIEDLWQVMYRGAFYRGGPIMMSAIAGVDQALWDIKGRAAGMPV
                     HQLLGGPCRDRIKVYSWVGGDRPADVAKAAREAVASGFTALKMNGAEEMQIVDSHARI
                     DKVVETVATVRQAIGPHIGLGVDFHGRVHRPMAKVLIRELEPYRLMFIEEPLLPEHID
                     AIGEVARNSSIPIALGERLYSRWDFRQVLESRVVDIIQPDLSHAGGITECRKIAAMAE
                     AYDVALAPHCPLGPIALASCLQIDAVSYNAFIQEQSLGIHYNGGGSDLLDYLVDPNVF
                     AYSDGMVAIPEGPGLGIEINEEAVIEAAKIGHRWRNPLWRHQDGSVAEW"
BASE COUNT         1521 a         2779 c         2785 g         1661 t
ORIGIN      
        1 ccggcaagcc ctttgccgcg ctcgatgcgc tggtggattg gttgccggca aaccttcccc
       61 aggacgatgc ccagctatcc atcgcccacg gcgatttcag gcttggaaac ctgctgtttc
      121 atcccacgcg gccggaggtc acaggcatcc tcgactggga gctttccacc cttggtcacc
      181 cgctcgccga tctcggcttc tgctgcatgc cgtggcacac ctcgccaagc gaatatggcg
      241 gcatcctcgg cctggactgg caggccctgg gcatcccgct acaggcgcgg tttgccgacc
      301 actactttgc ccatgcccgt ccgacggctc cgttgctgcc gttccacatt gtctttgcgc
      361 tgtttcgttt tgccgtcatc ttcgtcggca tcgccgaccg cgcacgcgct ggaaacgccg
      421 ccggcgccga tgcgcaaaac gtcgcgccgc tcgccggccg tttcgccgaa cgggcgctgg
      481 aaatcatcaa ggggaagcac attctctaga cagcgatccg gcgcgggacc gtctttctca
      541 agcgggcagg attctctttg cctgaagctc ttcaaaaatg ctgtcgaaca tctcttcggt
      601 gtccatgcat tcctctatcc cagcgaccgc ttcatgtcct cgcgcgccag gccgatcagg
      661 gcttgcatgg ctgcgcgggc ctggtccggt tcgccgcgct ggatcgcatc gtggactttt
      721 cggtgcatgg cgatggcgcc ttcgtgcgat tggctggcgc ggttggtcat ccggaagctc
      781 gccatcagtg cggcgcgcat ggcgacgatg aactgcctga gcacccagtt gccggaggcc
      841 tcgatgaccg ctgtatggaa agcgaggtcg gcgatgctcc agctttcggt gtcacggcct
      901 tccgattcga ccatgtccgc gaaggcggcg ccaatatcgg ccagcgctct tggcgaggcg
      961 catgatgcgg cgcgcgccgc cgcctcgggt tcgatcaaca ttcgggcatc gacgagatta
     1021 tcgtagaatt cgcggtcgcg tatgtgccgc aacgcggctt cgaggaccat cgtatccagc
     1081 agattccagc tggattgcgg aagaacgacc gttccggccc gtggccggga acggataagg
     1141 ccctgggcga ccagatgctg aaggccctcg cgcagggtcg gccggctgac gccgaaatga
     1201 ttgagcagca cttcttccgc aggcaggaaa gacccctgcg gccatgtgcc ggacacgatg
     1261 ttgtcgacaa gttcgtgcag cacgcccttc atggtcttgc gggtgatgtt gtgatcggga
     1321 gccagcgctg cgcgcggcaa ggtgtgggtg ggcatcgtac tcctgaccgc cggcctgcat
     1381 aaagcggctt gtcatactta acggagccgg cggttaagtg caatcttgga taaatatgat
     1441 ttatcttgac aaatgatcat atctatttat tcggtggccg cctaacctga gaggagttgg
     1501 gagttcatga ctatctggaa aaccgtggcg cttggtttca tggcggcatt gatcggcagc
     1561 agcgccgttc gcgccgacga tatcaagatc ggagccatct acatggatgc gcagggctat
     1621 tacgccggcg tacgcaaggg cattcaggac gccgccaagg aggcggacaa gaagctgcaa
     1681 atcatcgaga ccaacgcgca gggcgacgtt gcgaaggaaa gctccttcat cgatacgctg
     1741 atcgcctccg gcgttcaggc agtcatcacc tcgccggttt cggcggacgg ttcctaccgc
     1801 gccattcgcc gcgcccatga cgccggcatt ccggtgatct gctacaatac ctgtctcagc
     1861 gacggcgacc agaaggccta tgtctcggcc tatgccgtcg gcgatccatt cgagttcggc
     1921 aacaagctgg gtaagcaggc ggccgactat ttcgtcaagg ccggcatcaa ggagccgaag
     1981 atcgccgtcc tgaactgcga gttcgtcgag gtctgcgtca cgcgccgcga gggcttcgag
     2041 gccgcgctga aggaaaaagt gcccggctac aagatcgtcg acaaccagga aggcgcggtt
     2101 ctcgacaagg ccgtttcggt tgcaagcacc atcctgacgg ccaatcctga actcgacgcc
     2161 atcttcgggg aagccggcgg cgcgacgctg ggcgctgccc gcgccatccg cgcccagaac
     2221 agggtcggtc acgtcgtcgt ctttggcggc gacatgaccg ccgagatcgc caccgaactg
     2281 gggcagttcg acgtgatcaa ggccgtggtc gacatttccg gccagggcat gggccgtctg
     2341 gccttcgcgc aggcgctcaa cgtcatcgcg ggcaagcctc cgcaagacat caaggtgccc
     2401 tacaacatcg acctttacgc ttcctcggaa gatggcaagg gctggctgga agcccataag
     2461 gacggcattc cttaaatagc cggcgcctgc ctaatcgctg aaaagagtgg cggcgtgcag
     2521 ccgccgctcc tctcattcca tgaggtcacc tatgcaacag gcacagtccg ccttcgaggc
     2581 ccgacccgtc gtcgccagtc ttcagggctg ctcaaagcgc tatcccggcg tcgttgcgct
     2641 ggccgatgcg gattttgaaa tgcgcgccgg cgaagtgcgc gcaatgctcg gcaagaacgg
     2701 cgccggcaaa tcgaccttga tccggctgct tacgggggcg gaaacgcccg acaccggcac
     2761 ggtgacgata ggtggcgagg aactggccgg atcggattcc acgcgcgcgg ccgaggcttt
     2821 tgccaagggc gtgcgcgtcg tctaccagga actcagcctg gtcacggagc tgtccatcgc
     2881 cgaaaacctg ttcctggggc gttggccgcg ccgcgccggt gtgatccggc atggccagat
     2941 ggagcaggag gcgcgcgccg cgctcgatag cctgggtgtc gacctcgccc ccgggcggat
     3001 cgtctccagc ctcagcccgg cggagcggca gctggttgaa atcgcgcggg tgatgatcgg
     3061 aagcccgcgc ctagtcattc tcgatgagcc gacctcctcc cttgccgagg cggaggcgcg
     3121 caaggtattg gcggccgtca tgcgcatcgc ggctgcgggc atctccgtca tctatgtcag
     3181 ccacaggatg aacgagatcc gccagatcgc cgacacggtc accatcatgc gtgacggccg
     3241 catcatcgag acggttcccg tcgagggggc gcaaaccggc gatatcgtga agctgatgct
     3301 cggcgcagag atggatgcga acgaacatat ccagccgggg cttcgcggcg caccggtatt
     3361 ggagctgcgc gaggtttcgc ttgcgcccaa gctcgatcgc atctccttca ccctccatgc
     3421 cggcgaggtg ctgggcatcg ccggcttgct tggctccggg cgcaccgaac tgcttcagct
     3481 gatcatgggc ctgcgcaaca gatcggccgg cgaagtgctc gtcggcggca ggcgcgtcga
     3541 tggtgcttcc tacaggggca tggtgcggcg cggtttcggc ctgacgcctg aaagccggaa
     3601 ggaagagggc atcattccaa tgctcggggt cgatgaaaac acggtgatca cggatttcag
     3661 cgccgtcagc cgcaaccgca tattgtcggc gacccgtatc gccgaggcga cgcgccgtgt
     3721 gatcgaacgc ctgcaaatca agacggcgcg cacggatacg ccgatcggta cgctttcagg
     3781 cggcaaccag cagaaggtcg tcatcgggcg ctgggtccat gccggctcgc gcgttttgct
     3841 gctcgacgaa ccgacgcgcg gcgtcgatgt cgaggcaaag gctcaaatct atcgaattat
     3901 ccgcgaactc gccgcagagg gctgcgcggt gattttcgtt tcttccgaga tcgaggaatt
     3961 gccgctggtc tgcgatcgcg tgctggtcct tcgcgaccgc cgcatagcgg aggaaatagc
     4021 cgcccccggc atcgatcagg accggcttct ggccgcctgc atcgcggcag cttgaaacaa
     4081 ggacacagca atgagcgtaa cgaacgctga gaaccgccgc ttcgggcttg ccggctatat
     4141 gaacgagttg agccttctgt cggcgatcgt catcctttat ctggtctttg gactgacggc
     4201 gaatggcttc ctgagctttc ccaaccagat caacatcctg cgtgacgccg caaccatcgg
     4261 catcgcggcg tgggcggcga ccctgatcat catctgcggc gagatcgacg tttccaccgg
     4321 acccatggtg gcgttcatct ccgtcatcct tgcctatctg ctgcaatggg ggacgccgac
     4381 ggttctggcc ttcgcgctcg caatcgtgct cggcgggctg ctgggcgccc tggccggagc
     4441 gctgcgggcc tggttcgacg tgccgtcctt cgtcgccacg cttggattgt ggtcggcgct
     4501 gcgcggtctt gcccttttca tgaccgacgc gctgccggtg tccatcggcc gcaacgacat
     4561 cgtcgatctg ctcgaccgcc ccttcctggg actgccgccg gcggtgatcc tgatgctggc
     4621 gctgttcgtc ctgttcctgt tcatcagccg caagaccgcc ttcggcaggt ccgtctacgc
     4681 cgtcggcggc aaccctcatg cggcgctgct tgccggcatc aatgtcgcgc gcatacgcat
     4741 cctggtcttc gccatcggcg gggcgatggc cgccctgtcg ggcattcttc tgctttcccg
     4801 gctcggctcg ggcaatgcaa cggcggcttc cgggcttgaa ttcgacgtta tcgcggcggt
     4861 ggtcgtcggc ggcacggcgc tttcgggcgg acgcggctcc atggtcggaa ccctgctcgg
     4921 ggtccttgtc atcacgctga tcggcaacgg gctgatcctg ctcggcatca atccattcat
     4981 ccagcaggtg gtgcgcggcg ttatcatcgt ggtcgccgtg ctggccaaca tccaggccat
     5041 caaacgctcg cgcgcgcaga gaagctgaaa tgacggtcat tgcgcttcgc tccggcgaca
     5101 tgcgggcgag gattacgacg catggtggct cgatcctcag gctcgaccag ggtgcggtgc
     5161 cacttttgcg gcccgcggac gatgacgcgc tgccgatcga cagcgcctgc tacccgctgg
     5221 tccccttcgg taaccgcgtg cgcggcaacc gcttcacctt ccaggacaag gcctatacgc
     5281 tggcgcccaa tacagattgg gacgcgcatt atctgcatgg cgaaggctgg cgcagcgact
     5341 ggcgggtcgt taaccggcag gaggatcggc tcgttctttc gcatgagcat gacggttcgt
     5401 cgatgccatg gatttattcc gccgagcagc gcttcaactt gcgcccggac atgatcgagc
     5461 tggagatatc ggttaccaac cggggtaacg aggccatgcc gtttggcctc ggctggcatc
     5521 cctatttccc gatgacgccg caaaccacgc tccacacgcg cacgggacgc atgtggacgg
     5581 aggaggccgg ctggttgccg ggcaaggcgg tcgacctgcc gcaggacctg gatttttcgg
     5641 cgccgaaggg gctgccggat cattgggtca acaacggctt cgaggactgg tccggcgagg
     5701 cccggatcga ttggcccgaa cgcggccgcg ccttgcgcgt cgaggccgct ccgttgtttc
     5761 gggtgatgtt cctttttgtg tccagcacgt cgttcgatcc ttcctatcgg cgcgactttt
     5821 tcgcgctgga gccgatgagc caccttgccg acggccacaa catgcccgac ctcggcggcc
     5881 tcgcgccgct cgcgccgggc gagagcctgt cgggcagcct gcgcctgaaa gtggagcaca
     5941 gctgaatggc atcccgaagc tacgactaca ttcttgttgg cggcggcagt tccgcctgtg
     6001 cggcggctgc gcgtctgctt tcttccgggg cgtccgtcct catgctggag cgtggaccgg
     6061 ggcgctccaa tccgatcatg gattttccgg cgggctacat gaagttcctc gcccgcgata
     6121 cctatctggt gatgcacgac accgtgcctc agccgcaact cgacggccgc gcccccatcg
     6181 tccctcaagg gcgggtgctg ggtggcggcg gggccgttaa cgccatggtc tacatgcggg
     6241 gccagcgcga ggactacgcc gaatggaacc gcgccatcgg cggcgatggc aagggcgcat
     6301 gggactggga cgacctgctg ccgcattatc ttcgccagga agacaacgac cacctaggcg
     6361 gtccgctgca cggcgttggc ggccccttga aggtttccca tcttggccat accagcccga
     6421 tgacccgcgc ctggctcaag accatgcagc agatgggcat tccctacacg cccgacttca
     6481 acaatggcga ccctcgcggc gtcggcctga tgcaacacac gattgactgg gaaaagcgac
     6541 gccggtcgtc gcccgtcgag gcattcctgg cgccgtggct gaagaacccg cttctcgacc
     6601 tgcattgcca ggctaccgtc ggctccatct tgtgggaggg caaccgcgcc atcggcgtct
     6661 cgtggagaga gggtggtaag gcgcatgagg ccagggccgg agaagttctg atggcggccg
     6721 gcgcctacca gacgccgaag ctgctgatgc tgtccggggt cggccctgaa gaagagcttg
     6781 ccgcccatgg catcgccacg cgcatcgccc ttcccggtgt cggagccaat cttcaggatc
     6841 attacgagtg cccggtcgtc gccaccacca atggcgccta tggctattac ggccaggaca
     6901 aggggctgaa aatgctgctt gctggcctgc aatatctgtt gttcaggtcc ggtcccgtca
     6961 ccaccacggg cgtggaaagc tgcgccttcc tcgatccgac cggacaaggc ggccggccga
     7021 ccattcaaat gttctgcgtg ccgaccgtct atctcgaccg tgacgtgatg ggcgcggacc
     7081 ccggccatgg cgtcaccatc aactcgctgc tgttgcaacc caagtcgcgg gggcatgtga
     7141 agctgcgctc agccgatccc gacgcgctgc cgcttgtcga tctacagata ttcgctgagc
     7201 gtgccgacct ggacctgacg gttcaaggtt tccgcttcgc aagaagcgtg ctcgaacgct
     7261 cgccgctgcg cgacctcgtg aaagctgaaa tattccccgg ccgggacgtg gtttccgacg
     7321 aggccatcgc cgcccattgc cgacgcacgg tcaagaccgg ctaccacccg gtaggtacct
     7381 gccgcatggg aggcgacgac gacgccatgg ccgtgctcga cgcttcgctg aaagtgcgcg
     7441 gcgcgcaggg gttgcgcgtc atcgacgcct ccatgatgcc gaacatcgtt tccggcaata
     7501 ccaatgcggc ggtaatggcg gtggccgaca aggccgcaga cctgatcctt gggacgacac
     7561 catgaagatt gcaccatgaa aatcaccggg ctgaagactt tcatcgttcc gccacgttgg
     7621 ctgttcctga agatcgagac cgacgagggc gtctgcggct ggggcgagcc ggtcgtggaa
     7681 ggccgcgccc atacggtcga gaccgcggtt cgggaactgg ccgacatgct ggtcggacag
     7741 gacccgagca ggatcgagga tctgtggcag gtcatgtatc gtggtgcctt ctaccgtggc
     7801 ggcccgatca tgatgagcgc catagccgga gtcgatcagg ctctttggga catcaagggg
     7861 cgcgccgccg gcatgccggt ccaccagctg ctcggtggcc cgtgccgcga tcgcatcaag
     7921 gtttattcct gggtcggcgg cgaccgtccg gccgatgtgg cgaaggcggc gcgcgaggcg
     7981 gtcgcgagcg gcttcaccgc gctcaagatg aacggcgccg aggagatgca gatcgtcgac
     8041 agccacgcgc gcatcgacaa ggtggtcgag accgtggcca cggtgcggca ggccatcggc
     8101 ccgcatatcg gtctcggcgt cgatttccac ggccgggtcc accggccgat ggccaaggtt
     8161 cttatccgcg aattggaacc ctatcgcctg atgttcatcg aggaaccgtt gctgcccgag
     8221 cacatcgacg caatcggcga ggtggcccgc aattcttcga tacccatcgc actcggcgaa
     8281 cggctctatt cgcgctggga tttccgccag gtgctggaaa gccgggtcgt cgacatcatc
     8341 cagcccgacc tgagccatgc cggcggcatc accgagtgcc gcaagatcgc ggcgatggcg
     8401 gaagcctacg atgtggcgct cgcaccgcac tgcccgcttg ggccgatcgc gctcgcctcc
     8461 tgtctccaga tcgacgcggt cagctacaat gccttcatcc aggaacagtc gctcggcatc
     8521 cactataatg gcggcggcag cgatctgctc gactatctcg tcgatccgaa tgtctttgcc
     8581 tattcagacg gcatggttgc gattcccgaa gggccgggcc tcggtataga aataaacgag
     8641 gaagcggtga tcgaagcggc gaaaatcggc catcgctggc gcaacccact gtggcgccat
     8701 caggatggaa gcgtcgcgga gtggtgacat gacgagcaca agactg
//
LOCUS       DASYCS010000192         2846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_44358, whole
            genome shotgun sequence.
ACCESSION   DASYCS010000192 DASYCS010000000
VERSION     DASYCS010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44358"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VGM46_06200"
     CDS             <1..255
                     /locus_tag="VGM46_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006200138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192219.1"
                     /translation="DQGIADARQADAISPAEAHRLEMRAANISKVAERMADADHGRLP
                     RGQYHQLMRRVDHLDQRLLTDTGSGFVLGDGSDGGNYPNG"
     gene            387..1871
                     /locus_tag="VGM46_06205"
     CDS             387..1871
                     /locus_tag="VGM46_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018429982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HEY4192220.1"
                     /translation="MSFQKLDDLGHRLEALEHALAILGADEATHMAVGGGEKRAAAMA
                     SLAGMYHARATAPEIGDWIEAAAREALNEEQAAALGEFRRQYANLTCLSSEFVERQTG
                     ARLRCEQLWRDMRARNDWAGFQPALEGVVSLVREEAAMRADVLGLSPYDALMEQYDPG
                     NRAADITGAFAELKGFLKGFVPEALAVQEERLAKRPLKSLSGIYPVEKQCELGLAMMA
                     AVGFDLTHGSLSVSHHPFCGGVPSDVRITTRYKTSDFLSALMGVLHETGHALYEQNLP
                     KAWAHWPLGKARGMAVHESQSLFVEKQIGRNPAFWQWALPEVEKHLGESWSMDDMLAH
                     VHHVERGLIRVDADEVTYPLHVILRYELEQELVAGRLEVADIPEAWDAGMRDYLGIST
                     IDNPADGPMQDVHWAGGAFGYFPSYTLGAMMAAQQWAALTKEHPAADDDLARGDFTAV
                     NEWRRAKIWSQGSRWSTPELLERATGEKLNAAHFIGHLKRRYGR"
     gene            complement(1883..>2846)
                     /locus_tag="VGM46_06210"
     CDS             complement(1883..>2846)
                     /locus_tag="VGM46_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY4192221.1"
                     /translation="AGTLSPGTLGQFLLYSVFAAGALGALSEVWGELSQAAGAAERLT
                     EILVEVPGIQPPAHPKALPATAKGAVSFENVSFSYPARPDRAAVNGLTFSVKPGETIA
                     IVGPSGAGKSTVFSLILRFYDPESGNILVDGVDVREADPQAVRERIAIVPQDVTIFAG
                     SAGDNIGFGRPVASDAEIEAAARDALADEFILKLENGYASEVGERGVTLSGGQRQRIA
                     IARAILRDAPILLLDEATSALDAESETLVQTALERLMQGRTTIVIAHRLATVLKADRI
                     LVMDEGRIVEEGTHRSLVAKGGIYARLARLQFETGSSAFEGAAE"
BASE COUNT          508 a          869 c          988 g          481 t
ORIGIN      
        1 gaccagggta tcgcggatgc caggcaggcc gatgcgatca gcccggccga ggcgcaccgg
       61 cttgagatgc gggcagccaa catcagcaag gttgccgagc gtatggccga tgccgatcac
      121 ggccggttgc cccgcgggca atatcatcag ctcatgcgcc gggtcgatca tctcgaccag
      181 aggctgctga ccgatacggg cagcgggttc gttctcggcg acggctcgga cggcggcaat
      241 tatccgaacg gctgatttga caggatagaa ccgcccttgg gaaatcgccc gtgggcggtt
      301 ccatctgtcc gcacaggctt gccgccaccg cctgcttggt gtcctctccc gcctttcaag
      361 atgagaatca accaggcgga gccagcatgt ccttccagaa acttgacgac cttggccaca
      421 ggctcgaagc gctggaacat gcgctggcca ttcttggcgc ggacgaagcg acgcatatgg
      481 cggttggcgg cggcgagaag cgggcggcgg cgatggcgtc gctggccggc atgtaccatg
      541 cccgcgcgac tgccccggag atcggcgact ggatcgaagc cgcagcgcgg gaagcgctga
      601 acgaggagca ggcggccgcg cttggcgagt tcaggcggca atatgccaat ttgacctgcc
      661 tttcttccga gtttgtcgag cggcagaccg gggcgcgcct gcgctgcgag cagctttggc
      721 gcgacatgcg cgccaggaac gactgggcgg gcttccagcc ggcgctggag ggcgtcgtct
      781 cgctggtacg cgaggaagcg gcgatgcggg cggatgtgct cggcctcagc ccctatgacg
      841 cgctgatgga gcaatacgac cccggcaacc gggcggcgga cattaccggc gcctttgccg
      901 aattgaaggg cttcctgaaa ggtttcgttc ccgaagcgct ggcggtgcag gaggagaggc
      961 tggcgaaacg tccgctcaag tccttgtccg gcatctatcc cgtcgagaag cagtgcgagc
     1021 ttgggctggc gatgatggcg gcggtcggct tcgacctcac ccatggcagt ctttcggtgt
     1081 cgcaccatcc gttctgcggc ggcgtgccga gcgacgtgcg catcactacc cgctacaaga
     1141 catccgattt cctgtcggcg ctgatgggcg tgttgcacga gaccggccat gcgctctatg
     1201 agcagaacct gcccaaggcc tgggcgcact ggccgcttgg caaggcgcgc ggcatggcgg
     1261 tgcatgaaag ccagagcctg ttcgtggaaa agcagatcgg ccgcaacccg gcgttctggc
     1321 aatgggcgct gccggaggtg gagaagcatc tcggagaaag ctggtcgatg gacgacatgc
     1381 tggcgcatgt gcaccatgtc gagcgcggcc tgatccgcgt ggatgccgac gaggtgactt
     1441 atccgctgca tgtcatcttg cgctacgagc tggagcagga gcttgttgcg ggccggcttg
     1501 aggtcgccga cattcccgag gcctgggatg ccgggatgcg ggactatctg ggcatttcca
     1561 ccatcgacaa tccggcggac ggaccgatgc aggacgtaca ctgggccggc ggggcgttcg
     1621 gctatttccc gtcctatacg ctcggcgcca tgatggcggc gcagcaatgg gcggcgctga
     1681 cgaaagaaca cccggccgcc gatgacgatc tggccagggg cgacttcact gccgtcaacg
     1741 aatggcggcg cgccaaaata tggtcgcaag ggtcacgctg gtcgacaccg gaattgctgg
     1801 agcgcgccac cggcgaaaag ctgaacgctg cgcatttcat cggccatctg aagcggcgtt
     1861 acggcaggta gtcagcctgc cgctattccg cagccccctc gaaggcgctg gagccggttt
     1921 cgaactgaag ccgggcaagg cgcgcataga tgccgccctt ggcgacgagg ctgcgatggg
     1981 tgccttcctc gacgatgcgg ccttcatcca tcacgaggat gcggtcggcc ttcagcacgg
     2041 tggccagccg gtgggcgatg acgatggtgg tgcggccctg catcagccgt tccagcgcgg
     2101 tctgcaccaa ggtctcgctt tcggcgtcga gagcggaggt ggcttcgtcc agcagcagga
     2161 tgggcgcgtc gcgcaggatg gcgcgggcga tggctatgcg ctggcgctgg ccgccggaca
     2221 gggtcacgcc gcgctcgcca acctcgctgg cgtagccgtt ttccaacttg aggatgaatt
     2281 catcggcaag cgcgtccctg gcggccgctt cgatctccgc gtcggaagcg acaggccggc
     2341 caaagccgat attatcgccg gcgctgccgg cgaagatggt cacgtcctgc ggcacgatgg
     2401 cgatacgctc gcgcacggct tgcggatcgg cctcgcgcac atcgacgcca tcgacaagga
     2461 tattgccgct ctcagggtcg taaaagcgca agatgagcga aaagaccgtg ctcttgccag
     2521 cgccagaggg gccaacgatg gcaatcgtct cgccgggctt gaccgagaaa gtcaggccat
     2581 ttacggcggc gcggtcgggc ctggccggat aggagaagga tacgttctcg aaactgaccg
     2641 cgccttttgc cgtggcggga agcgcctttg gatgagccgg cggctggatg cccggaacct
     2701 cgaccagaat ttcggtcagg cgttcggcgg cgcctgcggc ctgcgaaagc tcgccccata
     2761 cttccgataa tgcgccgagc gcaccggcgg cgaacaccga atagagcagg aactggccaa
     2821 gcgtgccggg cgacagcgtg ccggca
//
LOCUS       DASYCS010000193         4280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_333410,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000193 DASYCS010000000
VERSION     DASYCS010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4280
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333410"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VGM46_06215"
     CDS             complement(<1..429)
                     /locus_tag="VGM46_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010915510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEY4192222.1"
                     /translation="MSNMMRALVKAKAEPGIWMEEVPLPEVGPNDVLIKIKKTAICGT
                     DVHIYNWDQWAQKTVPVPMVTGHEFVGTVADFGAAVTEYKIGQRVSGEGHIVCGHCRN
                     CRAGRGHLCRNTLGVGVNRPGAFGEYLAIPQHNVVPIPDDV"
     gene            complement(478..1665)
                     /locus_tag="VGM46_06220"
     CDS             complement(478..1665)
                     /locus_tag="VGM46_06220"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEY4192223.1"
                     /translation="MTTAFLSHLDTELDGLKSAGLYKSERVITSTQSAEIEVGGEKVL
                     NFCANNYLGLADSLELRDAAKKALDRYGYGMASVRFICGTQEEHKQLEARISGFLGME
                     DTILYGSCFDANGGLFETLLGEEDAVISDALNHASIIDGVRLSKAKRFRYANNDMAAL
                     EEELKNAQASRFKLIATDGVFSMDGIIANLRGVCDLAEKYGAMVMVDDSHAVGFVGKH
                     GRGSVEYCGVEGRVDIITGTLGKALGGASGGYTSGKRQVVDWLRQRSRPYLFSNTLMP
                     AIAGTSIKVFELVENGAALRQHLYSNAERFRTQMVKLGFTLAGAGHPIIPVMLGDASL
                     AQEMAARMLKRGIYVIGFSFPVVPKGQARIRTQMSAAHSTEDIDRAVEAFGIVGRELG
                     VIS"
     gene            1861..2322
                     /gene="queF"
                     /locus_tag="VGM46_06225"
     CDS             1861..2322
                     /gene="queF"
                     /locus_tag="VGM46_06225"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010915503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HEY4192224.1"
                     /translation="MTETKNLTQLGVRVETPEHPDRAVLETVPFTRGDGAPAIVRFTC
                     PEFTSLCPVTGQPDFAHIVIDYAPDQLLVESKSLKLFMVSFRNHGAFHEDCTVMIGRR
                     VVAATKPLWLRIAGYWYPRGGIPIDVFWQTGAPPEGAWLPDTGVAPYRGRG"
     gene            complement(2340..2876)
                     /locus_tag="VGM46_06230"
     CDS             complement(2340..2876)
                     /locus_tag="VGM46_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019857507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192225.1"
                     /translation="MRTRSFFVPILCCLALVTGSAAAEGLLGASPAKPMEGQPPAAAA
                     LPDKAVEPGSPLAAVKPFYDHIGLELDPRERNRFIDPARSVLDKADALEKSGEGDCLD
                     PNIALDNVVDQQAEIAKSLKMLDAAKNDDAKVIVAFVAQGKPHRLEWKLTKQGGDWKV
                     SDILSVTGEWALSQYQCE"
     gene            complement(2882..3361)
                     /locus_tag="VGM46_06235"
     CDS             complement(2882..3361)
                     /locus_tag="VGM46_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3828 domain-containing protein"
                     /protein_id="HEY4192226.1"
                     /translation="MRLSLLLAAFCVCSPGVALAGPASDAVRFFYIPQVRFEADASYR
                     ERFTEPVTKLFELNDAAIRKNPDQLACIDFDPGLDAQDFDQASVGKTLKLSEAVSGDS
                     AEVTATFDLFAEGEDAKREMVWSMKKIGGAWKVADIASRSNGWKLSQLDCMANDSAE"
     gene            complement(3395..>4280)
                     /locus_tag="VGM46_06240"
     CDS             complement(3395..>4280)
                     /locus_tag="VGM46_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010968845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEY4192227.1"
                     /translation="DFVDANCVILGNVNVNSPLVWDATMTTSLRAYARANQAAVIVPF
                     ILGGAMGPVTNAGAIAQSLAETMAGCALTQLERPGAPVIFGNFLSSMSLRSGSPTFGT
                     PEPAIGSMVIGQLARRLNLPLRCSGNFTTSKLPDAHAMNEGTMSMLAAVHCGANFILH
                     SAGFLDGLLSMSYEKFMMDADFCGALHTYLDGVRIDDNQLAFDAFKEVGPGSHFFGSQ
                     HTLENYETAFWDSEIADNENYEKWEAAGSEDAAVRANRQWKKALAEYVAPPLDQGIDE
                     ELTDFMSRKKADMQDAWY"
BASE COUNT          835 a         1335 c         1246 g          864 t
ORIGIN      
        1 aacgtcgtcg ggaataggca cgacattgtg ctgggggatg gcgagatatt caccgaacgc
       61 accgggccgg ttgacgccga cgcccagcgt attgcggcac agatggcccc tgcccgcgcg
      121 gcagttgcgg caatggccgc agactatgtg gccttcgccc gagacgcgct ggccgatctt
      181 gtattcggtc accgcagcgc cgaaatcggc aaccgtgccg acgaattcat ggccggtaac
      241 catcggcaca ggcacggttt tttgcgccca ctgatcccaa ttgtagatgt ggacgtcggt
      301 gccgcagatc gcggtcttct tgatcttgat caggacgtca ttggggccga cttccggcag
      361 cggcacctcc tccatccaga tacccggctc ggcctttgcc ttcaccagcg cccgcatcat
      421 gttcgacatg gctactttcc ttgaatctct tgattcaatg taattttttg ttgcgcgtca
      481 ggagatgacg ccaagttccc ggcccacaat gccaaacgct tccaccgcgc ggtcgatatc
      541 ctcggtcgaa tgcgccgccg acatctgggt gcggatgcgg gcctgcccct ttggcacgac
      601 cggaaacgaa aatccgatca cgtaaattcc acgcttcagc atccttgccg ccatttcctg
      661 cgccagcgaa gcatcgccaa gcatgaccgg aatgatcgga tgaccggcgc ccgccagcgt
      721 aaagcccagc ttgaccattt gggtgcgaaa ccgctccgca ttggaatata ggtgttggcg
      781 cagcgccgcg ccattctcga ccagttcgaa caccttgatc gaggttccgg cgattgccgg
      841 catcagcgtg ttggaaaaca ggtaagggcg cgaacgctgg cgcaaccagt cgaccacctg
      901 gcgcttgccg gatgtgtagc cgcccgatgc gccgcccagc gccttgccca atgtgccggt
      961 gatgatgtcg acccgcccct cgacgccgca atactcgact gacccacgcc cgtgcttgcc
     1021 gacgaagcca accgcgtggc tatcgtccac catcaccatg gcgccgtatt tttccgcgag
     1081 gtcgcacacg ccgcgcaaat tggcgatgat gccgtccatc gagaaaacgc cgtcggtagc
     1141 aatcagcttg aagcggcttg cctgtgcatt cttcaactcc tcttccagcg ctgccatgtc
     1201 gttattggcg taacgaaagc gctttgcctt ggacaagcgc acgccgtcga tgatcgaggc
     1261 atggttcaac gcatcggaaa tgaccgcgtc ctcttcgcca agcagggttt cgaacagccc
     1321 gccattggcg tcgaaacagg aaccgtagag gatcgtgtcc tccatgccga gaaagccgga
     1381 aatccgggct tccagttgct tgtgctcttc ttgcgtcccg caaatgaaac gcaccgaggc
     1441 catgccgtag ccgtaacggt cgagcgcctt tttcgcggcg tcgcgcagtt ccaggctgtc
     1501 ggcaaggccg agatagttgt tggcgcagaa attcagcacc ttttcgccgc cgacttctat
     1561 ctcggcgctt tgcgtggagg tgatgacccg ctcggactta tagagtccag ccgacttcaa
     1621 gccatcgagt tcggtatcca gatgcgaaag aaaagctgtg gtcatggccg gccccgtgct
     1681 gattttgacg agtgttgcgc cgcgcgggag aaaaaatcta ccgcaaaacg acgggtattg
     1741 gctgctgtag cgacgcggtt gcccggcgat cctggggcaa ttttacgcgg gccgggtaag
     1801 gcaagccatc gtgctgaagc cgggtttcgt gctaggagcg ccgcttccaa ggagcctgcc
     1861 atgacagaaa ctaaaaatct cacccagctt ggcgtcaggg ttgaaacgcc cgagcacccc
     1921 gatcgcgcgg tgctggaaac ggtgccgttc acgcgaggcg acggcgcccc ggccatcgtg
     1981 cgcttcacct gcccggaatt cacatcgctt tgcccggtca ccggccagcc ggatttcgcc
     2041 catatcgtca tcgactatgc gcccgaccag ttgctggtgg aatcgaaatc cttgaaactg
     2101 ttcatggttt catttcgcaa ccatggtgcg ttccatgaag actgcacggt gatgatcggc
     2161 cggcgcgtcg tcgcggcaac caagccgcta tggctgcgca tagccggcta ctggtatcca
     2221 cgcggcggca ttcccatcga cgttttctgg caaaccggag cgccgccgga aggcgcgtgg
     2281 ctgcccgata ccggtgtcgc gccctatcgc ggcaggggtt gaccgcgtag cgggaaccgt
     2341 tattcgcact gatattggct cagcgcccat tccccggtta ccgacagaat atcggaaacc
     2401 ttccaatcgc cgccttgctt cgtcagcttc cattccaggc ggtgcggctt gccctgcgcg
     2461 acaaaggcga cgatgacctt ggcgtcgtcg ttctttgccg cgtcaagcat cttgaggctt
     2521 ttggcgatct ccgcctgctg gtccacgaca ttgtcgagcg cgatattggg atcgagacag
     2581 tcgccttcgc cggatttttc gagcgcatcg gccttgtcga gcacgctgcg agccggatcg
     2641 atgaaacgat tgcgctcgcg cggatcgagt tcaagcccga tatggtcgta aaagggcttg
     2701 accgcagcca gaggagaacc gggttcaacc gctttgtcgg gtagagcagc ggcggctggc
     2761 ggctggcctt ccatgggctt ggcaggcgat gcaccaagaa gcccctcggc ggcagcgctg
     2821 ccagtaacca gcgccaggca gcataatatc ggcacgaaaa agctgcgtgt ccgcacgggt
     2881 tttactcggc gctgtcgttg gccatacagt ccagttggct tagtttccag ccgttagacc
     2941 tggaggcgat gtcagcaacc ttccacgcgc cgccgatctt tttcatcgac cagaccattt
     3001 cacgctttgc atcctcgcct tccgcgaaga gatcgaaagt cgccgtcacc tcggcgctgt
     3061 cgccgctgac cgcctcggat agtttgagcg tcttgcctac gcttgcctgg tcgaaatcct
     3121 gcgcatccag acccggatcg aaatcgatgc aagccagctg gtccgggttc ttgcggatcg
     3181 cggcatcgtt cagttcgaac agtttcgtca ccggttcggt aaagcgttcc cgataggaag
     3241 cgtcagcctc gaacctgacc tgcggaatgt agaaaaacct gaccgcgtcc gacgccgggc
     3301 cggccagcgc cacgccaggt gaacagacgc aaaatgctgc gagcaataag gacagtctca
     3361 tccgtgattc ccgatttgca tgaagccatg gcaatcaata ccacgcatcc tgcatatcgg
     3421 cttttttgcg gctcatgaaa tcggtaagct cttcgtcgat gccctggtcg agcggcggcg
     3481 cgacatattc ggccaaggcc tttttccact gccggttggc gcggacggcg gcatcctctg
     3541 aacccgccgc ctcccacttc tcgtaattct cgttgtcggc gatctccgag tcccagaaag
     3601 ccgtctcgta attttccagc gtgtgctgcg agccaaagaa atggctgccc ggcccgacct
     3661 ccttgaaagc gtcgaacgcc aactggttgt cgtcgatcct gacaccgtcg agataggtgt
     3721 gcagcgctcc gcaaaaatca gcgtccatca tgaatttctc ataggacatc gacagcaggc
     3781 catcgaggaa accggcggaa tgcaggatga aattcgcccc gcaatgcacc gccgccagca
     3841 tcgacatcgt gccttcgttc atggcgtgcg catcgggaag cttggaggtg gtgaaattgc
     3901 ctgaacaacg cagcggcagg ttcagccgcc gggccagctg gccgatcacc atcgagccga
     3961 tggccggttc cggcgtgccg aaggtcggcg agccggagcg cagcgacatc gaagacagga
     4021 aattgccgaa gatgactggc gcgcccggcc gctccagttg cgtcagcgcg catccagcca
     4081 tcgtttcggc cagcgattgc gcaatcgcgc cggcattggt caccggcccc atggctccgc
     4141 cgagaatgaa cggcacgatg accgccgcct ggttggcgcg ggcataggcg cgcagcgaag
     4201 tcgtcatcgt cgcatcccag accagcggcg aattgacgtt gacgttgccc aggataacgc
     4261 agttcgcgtc aacgaagtcg
//
LOCUS       DASYCS010000194         1787 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4396397,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000194 DASYCS010000000
VERSION     DASYCS010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4396397"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            295..>1787
                     /locus_tag="VGM46_06245"
     CDS             295..>1787
                     /locus_tag="VGM46_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009338484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY4192228.1"
                     /translation="MNIRNIAIIAHVDHGKTTLVDQLLKQSGSVRENQHMAERAMDSN
                     DQERERGITILAKCTSVEWHGTRINTVDTPGHADFGGEVERILSMVDGVVLLVDAAES
                     VMPQTKFVLSKALKLGLKPIVVINKIDRQDAQPKETLESIFDLFLALEANDDQLNFHY
                     LYASGRNGWAVKELEDERKNLDPLFQLIVDDVPAPKPQALASEEHPFKMLVTTLEADN
                     FLGRILTGRIESGIITTNRNIKALNRDGEIIEKTRVTKLLAFRGLARVPVDRAEAGDI
                     VAIAGLEDATVADTLCDLTVEEAIHAQPIDPPTLSMTISVNDSPYAGREGDKVQSRVI
                     RERLLREAEGNVALKVTETGDGDFEVAGRGELQLGVLIESMRREGFELSISRPRVLFK
                     TENGEKMEPIEEVTVDVDDAYTGTVIEKISQRKGEMTDMRPMGAGKTRVIFRGPARGL
                     IGYHGEFLTDTRGTGVMNRMFVEYAPHKGPLAGRINGVLISTEQGEAV"
BASE COUNT          382 a          573 c          520 g          312 t
ORIGIN      
        1 cgccaacatt acattcgaaa acccgcccat cccacgcccg tagcgaggat ttttacaagc
       61 cacgcacggc cttagcggtg ttttggtgcc cttattgccg tgttctatag ggcaaaactc
      121 tctcacaatt gtctcaaaaa aaagcttgcc ctacgtcatg tagccgcccg aatacttcat
      181 taacatgctg aaatcattgt tattaatttg tagcgctgcg acttttgcag cgcacattgc
      241 gggcgtcaaa gcccctgtct ataagccgcg cctgaccccc cgagcgcccg atccatgaat
      301 atccgtaaca tcgccatcat cgcccatgtc gaccatggca aaaccaccct ggtcgaccag
      361 ctgttgaaac agtcgggctc cgtgcgcgaa aaccagcaca tggccgaacg cgccatggat
      421 tccaacgacc aggagcgcga gcgcggcatc accattctgg ccaaatgcac cagcgtcgaa
      481 tggcatggca cgcgtatcaa caccgtggac acgcccggcc atgccgattt cggcggcgaa
      541 gtggaacgca tcctgtcgat ggtggacggc gttgtgctgt tggtggacgc ggcggaaagc
      601 gtcatgcccc agaccaaatt cgtgctttcc aaagcgctga agctgggcct caagcccatc
      661 gtggtcatca acaagatcga tcggcaggac gcccagccca aggaaacgct ggaatcgatc
      721 ttcgacctgt tcctggcgct ggaagccaat gacgaccagc tgaacttcca ttatctctac
      781 gcgtccggcc gcaatggctg ggcggtgaag gagttggaag acgagcgcaa gaatctcgac
      841 ccgctgttcc agctgatcgt ggacgatgtg cccgccccca agccgcaggc gttggcgagc
      901 gaagagcatc ccttcaagat gctggtcacc acgctggagg ccgacaactt cctgggccgc
      961 attctgaccg gccgcattga atcgggcatc atcaccacca accgcaatat caaagcgctg
     1021 aaccgcgacg gcgagatcat cgagaagacg cgcgtcacca agctgctggc atttcgcggc
     1081 ctggcgcgcg tgccggtgga ccgcgccgaa gccggcgaca tcgtcgccat tgccggcctg
     1141 gaagacgcca ccgtcgccga cacgctgtgc gatctcaccg tggaagaggc catccacgcc
     1201 cagccgatcg atccgccgac cctgtccatg accatctcgg tcaacgattc cccttatgcg
     1261 ggccgcgaag gcgacaaggt gcagagccgc gtcatccgtg agcgcttgct gcgcgaggcc
     1321 gaaggcaatg tcgcgctgaa ggtgaccgag accggcgacg gcgactttga agtcgccggc
     1381 cgcggcgaac tgcagcttgg cgtgctgatc gaatccatgc gccgcgaggg cttcgagctg
     1441 tcgatcagcc gcccgcgcgt gctgttcaag accgaaaacg gcgagaagat ggagccgatc
     1501 gaggaagtca cggtcgatgt cgacgacgcc tataccggca cggttatcga gaagatttcc
     1561 cagcgcaagg gcgagatgac cgacatgcgc ccgatgggcg ccggcaagac ccgcgtgatc
     1621 ttccgcggcc ccgcccgcgg cctgatcggc tatcacggcg aatttctcac cgacacccgc
     1681 ggcaccggcg tgatgaaccg catgttcgtg gagtatgccc cgcacaaggg cccgctggcc
     1741 ggccgcatca acggtgtgct gatctccacg gagcagggtg aggcggt
//
LOCUS       DASYCS010000195        11136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1931239,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000195 DASYCS010000000
VERSION     DASYCS010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 11136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11136
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1931239"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2841)
                     /locus_tag="VGM46_06250"
     CDS             complement(<1..2841)
                     /locus_tag="VGM46_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010913009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEY4192229.1"
                     /translation="MTLHDVALDDKFDLAKERVFLSGAQALVRMLLMQRERDRQAGLN
                     TAGFVSGYRGSPLGGLDLQLWKARDQLGAADIVFQPGLNEELAATACWGSQQAEVLGE
                     GRHDGVFSVWYGKGPGVDRSGDVFRHANLAGSSRHGGVLALMGDDHMAESSTNAHATE
                     FAFVDAMVPILNPAGVQEVIDYGLYGIALSRFAGTWGAIKCVKDNIESTASVDASLGR
                     VNIVLPEFDMPPGGLNIRNEIDFLGQEERLHEYKRAAASAFIRANGLNRLVYSGGRNP
                     KIGIVTVGKSYLDVRQALEDIGIDEAQANRIGVSLFKVGCPWPLDFQHVADFARGLDT
                     IVVVEEKRSLIEVQLRENLYGTAIQPNIVGKKDERGDWLFPAKGALDPNDIAIALGER
                     ILNTIGHSEEVAARVANLRQFQAMLADTVDVGSRTPFFCSGCPHNSSTKVPDGSIAAA
                     GIGCHFMSLWMDRSTIGFTAMGGEGAQWIGQAPFSKRGHMFQNLGDGTYNHSGSLALR
                     FALSSGVNITYKILYNDAVAMTGGQPHEGGLTVDMIARQIRAEGVERIAIVSDEPEKY
                     AGKIEFPAGATIHHRRDMDAVQRELRDVKGVSVLIYDQTCAAEKRRRRKRGAYPDPDR
                     RIFVNELVCEGCGDCGVKSNCVSIQPVETEFGRKRRIDQSSCNKDFSCIDGFCPSFVT
                     VHGAKIRKAEGLAGNADPLDGVPDPLRFALGREGWAGIIDGVGGTGVVTVGAILGMAA
                     HLEGKGCGMIDMAGLAQKGGSVFTHVRVANTPDDIHAIRVSAGKADLVLGCDLVVSGA
                     KKVLAAVRENHTVFLVNTAEIMPGEFTRSADFSLPVERLKKAIRKAAGEEKAHFFDAT
                     RTATALFGNSLGANMFMLGFAYQHGGLPLSAEAVEKAIELNAQAVAMNIAAFRWGRRA
                     AHRPEFVQGLVEKPGMAAAPAAVSQTLDERIAR"
     gene            complement(3042..3572)
                     /locus_tag="VGM46_06255"
     CDS             complement(3042..3572)
                     /locus_tag="VGM46_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015314284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192230.1"
                     /translation="MCRRSFHAALALALLIGASVLGACRDTGKDELFAVAGRMFEFNY
                     RLGRATYVVTLNPLRPMGEGQVAIAHFQNPAGGDPIEVRQPIWPKMPHVTLTSPALTC
                     VVKDKPYAVSIRIEDKDGALLQALETTLVSSLDQSVLPDRPLVTGTVYALNPELEGHP
                     DGKLPNEPKPVCPDNG"
     gene            complement(3565..4173)
                     /locus_tag="VGM46_06260"
     CDS             complement(3565..4173)
                     /locus_tag="VGM46_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HEY4192231.1"
                     /translation="MTVSWIPHRFSGGVLALDTANTVVLRIDTQKTFDRFDDPAEIGR
                     FSEAASIFCAAELGGRKLEVRDPHKIAPVVVSIREATDRLFRGAAKERAMESSALPAF
                     LNACAAGLSASSTLVGAPGKPFGDPATPIAFEAALAVSALSLLAPNAVERLKICPNCG
                     WLFMDRSRNSSRLWCDMAVCGNRRKASRHYGRRKSAREVFDV"
     gene            4264..5169
                     /locus_tag="VGM46_06265"
     CDS             4264..5169
                     /locus_tag="VGM46_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY4192232.1"
                     /translation="MLYFLQQVLNGLHSGALYALLAFGYALTNGVLHRTNLAHGAVFA
                     FCGQAMILAAVYGYDVLWLTLWASVALGIAAALLYSVLLGHLLSRHVFEPLSRRSPNT
                     LVAATLGVLLALMEFARIAADTHDFWLPPMLSQPFVFAQDGAFRATLTGIQLLDCAVV
                     AVTVALGAWALARSSFGRKWRAVLDDPFAAALCGVYTVRIFRGAVLAGGLCAALAGIM
                     AGLYYGNIGFGDGLTYGLKILFVTAVGGFLSPVRAALGAVAFGIGESLWAGYFPIEWR
                     DAWMYFLLVAMLVLVGAGREENKAV"
     gene            5360..5944
                     /locus_tag="VGM46_06270"
     CDS             5360..5944
                     /locus_tag="VGM46_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018095209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEY4192233.1"
                     /translation="MGFLLALSRGIDRLNEFVGKSVSWLILAAILVSAGNAIIRKVFD
                     MSSNAWLELQWYLFGAAFLLAAAHALKQNEHIRIDIVYGLFSRRVQHWVDLFGHVFFL
                     MPFSALMVVYLVPYVTRSVESGEMSTNAGGLIIWPAKALLLAGFLLLALQGVSEIVKK
                     IAVMRGKTDDPNPFIPAHEQAELEAKALAGEVRA"
     gene            5941..7701
                     /locus_tag="VGM46_06275"
     CDS             5941..7701
                     /locus_tag="VGM46_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEY4192234.1"
                     /translation="MIELIAQNMAPIMFCSLIVFMLIGYPVAFSLAANGLLFFLIGVE
                     LAPYSNGSINLSWPLLHALPDRFYGGVMANETLLAIPFFTFMGIVLERSGMAEDLLDT
                     IGQLFGPIRGGLAYAVIIVGALLAATTGVVAASVIAMGLISLPIMLRYGYDRRLASGV
                     IAASGTLAQIIPPSLVLIVLADQLGRSVGDMYAGALIPGLVLTGLYMGYILIVSIVRP
                     KLVPALPLEARTLGHGVLSLIAALIATALVSYAAYRYLAPTQGENADILAATVGIIFI
                     YCVALADKTFKFNLMSRLAQQVVIVLIPPLALIFLVLGTIFLGIATPTEGGAMGAVGA
                     LVMAAAKRRLSLDLVKQALAATTRLSSFVLFILIGARVFSLTFYGVNGHLWVEHLLVS
                     LPGGEVGFLIFVNLLVFFLAFFLDFFELAFIIVPLLAPAAEKLGIDLIWFGVLLGINM
                     QTSFMHPPFGFALFYLRSVAARVPYLDRITGKRTAPVTTGQIYWGAVPFVCIQILMIG
                     LTIAFPGMVMHYKGKPVDPGSIEIKMPDMPGLPPLGAPQSGGAGATPDLSAPPNFGAP
                     APAKPATPAVPDLSQPPSFN"
     gene            complement(7762..8871)
                     /locus_tag="VGM46_06280"
     CDS             complement(7762..8871)
                     /locus_tag="VGM46_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEY4192235.1"
                     /translation="MDRRSFIRKAGVTGAGAAATVATLAAPAIAQSNPKVTWRLTSSF
                     PKSLDTIYGGAETFSKMLSEATDGNFQVQVFAAGELVPGLQAADATTAGTVEACHTVG
                     YYYWGKDPVWALGAAVPFALNARGMNAWLYHGGGNDLYNEFLATQGLVGFPGGNTGTQ
                     MGGWFRKEINTVADLSGLKMRIGGFAGKVVERLGVVPQQLAGGDIYPALEKGTIDAAE
                     WVGPYDDEKLGFQKVAPYYYYPGWWEGGPTVHLMFNKAKYDELSPTYKSLLQTAARAT
                     DADMLQKYDYLNSKAVKSLVANGALLRPFSQEIMLACFDKSNEVYAEMMNTNPTFKKI
                     WESIVAFRKEHYLWQQVAEYNFDTFMMIQQRAGKL"
     gene            9115..9888
                     /locus_tag="VGM46_06285"
     CDS             9115..9888
                     /locus_tag="VGM46_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEY4192236.1"
                     /translation="MSQPLVAVSTDVKQFENYTWHAAPQQYLEAAISTAGVFPLLVPS
                     FGDRLDLDELLSRVDGVMMTGSKSNVYPALYGGDASEANGPYDHARDATTLPLIRSAI
                     ERGVPLLAICRGIQEMNVALGGTLGTEIQEREGALDHRAPPSDNQDERFAIRQNVSIK
                     AGSCLAGVFGAGDIIVNSVHRQGVDRLGPKLQVEAVAEDGTVEAVSARDSRAFAVGVQ
                     WHPEYWAKSDDASQRIFQAFGEAVRQHAAGKIAARAAAE"
     gene            complement(9915..10904)
                     /locus_tag="VGM46_06290"
     CDS             complement(9915..10904)
                     /locus_tag="VGM46_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEY4192237.1"
                     /translation="MAEDLRSITILVPGKLHEHAVARISDTFKLVSIDRIDPALVTPE
                     LRDTVRGVAAAPAIGGSFVGPDFLRALPKAEIIANFGVGYDGIDAAYAGRQGIMVTNT
                     PDVLTEEVADTAIGLLISTIREFSPAERWLREGDWKRNGNYPQSRLTLRGRTVGIFGL
                     GRIGIAVARRLEAFGLAIAYHNRRPVEGVAYAYHATLKDLAEAVDTLICIAPGGAATE
                     NAVNAQILSALGAEGVLVNIGRGSTVDEAALAKALADGTIAAAGLDVFADEPNVPQSL
                     LDAPNTLLLPHIASASIHTRRAMADLCVDNLIAWFRDGNALTPVPETVGVKPK"
     gene            complement(10929..>11136)
                     /locus_tag="VGM46_06295"
     CDS             complement(10929..>11136)
                     /locus_tag="VGM46_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY4192238.1"
                     /translation="VTGYDDLEEASIATPALTTVWNGQREVGRRAASALLDKLNGESV
                     RTPQELIKPELHVRQSTGRPVERT"
BASE COUNT         1912 a         3535 c         3504 g         2185 t
ORIGIN      
        1 gcgcgcaatg cgttcatcca gcgtttggga aaccgcggcc ggcgcggccg ccatgccagg
       61 cttttcaacc aagccctgga cgaattctgg ccggtgggcg gcgcgccggc cccagcggaa
      121 cgcggcgatg ttcatggcga ctgcctgcgc gttcagttcg atggctttct cgacggcttc
      181 ggccgatagc ggcaggccgc catgctggta ggcaaagccc agcatgaaca tgttggcgcc
      241 aagcgaattg ccgaacagcg ccgtcgcggt gcgcgttgcg tcgaagaagt gcgccttttc
      301 ttcacctgcc gccttgcgga tcgctttttt caggcgctcc accggcagcg agaaatcggc
      361 ggagcgggtg aattcgcccg gcatgatttc ggccgtgttg accaggaata ccgtatggtt
      421 ctcgcgcacg gccgccagca ctttttttgc gccggaaaca acgaggtcgc agccgagcac
      481 cagatcggcc ttgccggccg acacgcgaat cgcgtggatg tcgtcgggcg tattggccac
      541 gcgtacatgg gtgaataccg agccgccttt ctgagcaagg cctgccatgt cgatcatgcc
      601 gcagcccttg ccttcgagat gggcggccat gccgaggatg gcgccgacgg taacgacgcc
      661 ggtaccgccg acgccgtcga tgatgccggc ccagccttcg cggccaagcg cgaaccgcaa
      721 cggatcaggc acgccgtcaa gcggatcggc gttgccggca agcccttcgg ccttcctgat
      781 tttcgcgcca tggacagtga cgaaggacgg gcaaaagccg tcgatacagg aaaaatcctt
      841 gttgcaggac gactggtcga tgcgcctttt gcggccgaac tcggtctcga ccggctggat
      901 cgagacacaa ttggacttca cgccgcagtc gccgcagcct tcgcagacga gttcgttgac
      961 gaagatgcgc ctgtcgggat cgggataggc gccgcgcttg cggcggcggc gtttttcggc
     1021 ggcgcaggtc tggtcgtaga tcagcaccga cacgcccttg acgtcgcgca gttcacgctg
     1081 gacggcgtcc atgtcgcggc gatggtggat ggtcgcgccg gctgggaact cgatcttgcc
     1141 ggcgtatttt tccggttcgt ccgagacgat ggcgatgcgc tcgacgccct cggcgcgtat
     1201 ctgccttgcg atcatgtcga ccgtcagccc gccctcatgc ggctggccgc cggtcatggc
     1261 gacggcatcg ttgtagagga tcttgtaggt gatgttgacg ccgctcgaca gcgcgaagcg
     1321 cagcgccagc gagccggaat ggttgtaggt gccgtcgccg agattctgga acatgtggcc
     1381 gcgtttggag aacggcgcct ggccgatcca ttgcgcgccc tcgcccccca tggcggtgaa
     1441 gccgatggtc gagcggtcca tccacagcga catgaaatgg cagccgatgc cggcggcggc
     1501 aatcgagccg tccggcacct tggtcgatga attatgcggg caacccgaac agaagaacgg
     1561 cgtgcgcgag ccgacatcga ccgtgtcggc cagcatcgcc tggaactggc gcagattggc
     1621 gacgcgggcg gcaacctcct cggaatggcc gatggtgttc aggatgcgct cgcccagcgc
     1681 gatggcgata tcgttgggat cgagcgcgcc cttggcgggg aacagccagt cgccgcgttc
     1741 atccttctta ccgacgatgt tcggctggat ggcggtgccg tagaggtttt cgcgcagttg
     1801 cacctcgatc agcgagcgct tttcctcgac gacgacaatc gtatcgaggc cgcgcgcgaa
     1861 gtcggccaca tgctggaaat cgagcggcca cggacagccg accttgaaca ggctgacgcc
     1921 gatgcgattt gcctgcgcct cgtctatgcc gatgtcttcc agcgcctggc gcacgtcgag
     1981 atagctcttg ccgacggtga cgatgccgat cttggggttg cggccaccgg aatagaccag
     2041 ccggttcagg ccgtttgcgc ggatgaaggc gctggcggcg gcgcgcttat attcatgcag
     2101 ccgttcttcc tggccgagaa aatcgatctc gttacggatg ttgaggccgc cgggcggcat
     2161 gtcgaactcg ggaaggacga tgttgacccg gccaagcgag gcatcgacgg aagccgtcga
     2221 ttcgatattg tccttgacgc attttatggc gccccaggtg ccggcaaaac gcgacagggc
     2281 gatgccataa agcccgtaat cgatgacttc ctgaacgccg gccggattga ggatcggcac
     2341 cattgcatcg acgaaggcga actcggtggc atgcgcattg gtggacgatt cggccatgtg
     2401 gtcgtcgccc atcaaggcaa ggacaccgcc atgccgcgac gagccggcca gattggcgtg
     2461 gcgaaaaacg tcgccggagc ggtcgacgcc cggccccttg ccgtaccaga cggaaaatac
     2521 gccgtcatgg cggccctcgc ccagcacttc cgcctgttgc gaaccccagc aggcggtggc
     2581 ggccagctct tcgttcaaac cgggctggaa cacgatatcg gcggcgccaa gctggtccct
     2641 ggccttccac agctgcaaat ccaggccgcc cagcggcgag ccgcgatagc cggagacgaa
     2701 gccggcggtg ttcaggcccg cttgccggtc gcgctcgcgc tgcatgagca gcatgcgcac
     2761 cagtgcctgc gcgcccgaaa ggaagacgcg ctctttggcg agatcgaact tgtcgtcgag
     2821 cgcgacatca tgcagcgtca ttgtacttcc tctgcggaga ccgcaccgag accgcggcca
     2881 gcgtcgagat ggccagcagt tagccgccac tgtttatccg cttgccgggc aggtcaaacg
     2941 aaaacaaatt gatcgaacgg cgtccaaagg ccgcgatggc ggtggcagcg ttctatacaa
     3001 gaaggcgcaa aaccgcgacc gaaatttgtc ggataaagcg tctatccgtt gtcggggcag
     3061 accggttttg gttcattcgg caacttgccg tccgggtggc cctccagttc gggattgagc
     3121 gcatagacgg ttcccgtcac cagcgggcgg tcgggcaaca cggattggtc gagcgacgac
     3181 accagcgtcg tttccagcgc ctggagcaat gcaccgtcct tgtcctcgat gcggatggaa
     3241 acggcatagg gcttgtcctt gacgacgcag gtgagcgccg ggctggtcaa agtgacatgg
     3301 ggcatcttgg gccagatcgg ctggcggacc tcgatgggat cgccgcccgc cgggttctgg
     3361 aaatgggcga ttgccacctg cccttccccc atcgggcgaa ggggattgag cgtgacgaca
     3421 taggtggcgc gcccgagccg gtagttgaac tcgaacatgc gaccggcgac ggcgaacaac
     3481 tcatccttgc cggtgtcgcg gcaggcacca agcacggaag cgccgatcag caatgcaagt
     3541 gcgagagccg cgtgaaatga ccgtctacac atcgaaaacc tcccttgccg attttctgcg
     3601 gccgtaatgg cggcttgcct tgcgtcggtt gccgcacacc gccatgtcgc accacaggcg
     3661 gcttgaattg cggctcctgt ccatgaacag ccagccgcaa ttggggcata tcttcagtcg
     3721 ctcgactgca ttaggcgcaa gcaatgacag ggcggaaacg gccagcgccg cctcgaaggc
     3781 gatgggcgtc gccgggtcgc cgaaaggttt tccgggtgcg ccgacaaggg tggaactggc
     3841 ggacaggcca gccgcgcagg cattcaggaa agccggcaag gcgctgcttt ccatcgcgcg
     3901 ttccttggca gcgccgcgaa agagcctgtc ggtcgcttcg cggatcgaca ccacgacggg
     3961 tgctatcttg tgcgggtcgc gcacttccag cttccgcccg ccaagctcgg ctgcgcaaaa
     4021 gatgctggct gcttcagaaa agcgcccgat ctcggccgga tcgtcgaagc gatcgaacgt
     4081 cttttgcgtg tcgatgcgca gcaccaccgt attggccgta tccagcgcca gcacgccgcc
     4141 ggagaagcga tggggaatcc acgaaaccgt catggccaga aatctaactg gtaaattgcg
     4201 tttgacaagt tagattttgc cgagcagcat catcgcgccc cagtgctctg acagcggttg
     4261 cgcatgctct atttcctcca gcaggttctg aacgggctgc attcgggcgc gctctatgcc
     4321 ctgctggcgt tcggctatgc gctgaccaat ggcgtcctgc accgcaccaa ccttgcccat
     4381 ggcgcggtgt tcgccttttg cgggcaggcg atgatcctcg ccgccgtcta tggctatgac
     4441 gtgctgtggc tgacgctctg ggcctccgtc gcgctcggca tcgccgccgc gctgctctat
     4501 tcggtactgc ttggccattt gctgtcgcgc catgtgttcg agccgctcag ccggcgctcg
     4561 cccaacacgc tcgtcgccgc cacgctcggc gtgctgctgg cgctgatgga attcgcccgc
     4621 atcgccgccg acacccatga tttctggctg ccgccgatgc tgtcgcagcc cttcgtcttc
     4681 gcgcaggacg gcgctttccg cgccacgctg acgggcatcc agctcctcga ctgtgccgtc
     4741 gtcgccgtaa ccgtggcgct gggtgcgtgg gcgctggcac ggtcaagctt cggccggaaa
     4801 tggcgcgccg tgctggacga tccgttcgcc gcagcccttt gcggcgtcta cacggttcgc
     4861 atctttcgcg gcgcggtgct tgccggcggc ttgtgtgcgg cgctggccgg catcatggcc
     4921 gggctttact acggcaatat cggctttggc gacgggctga cctatgggct gaaaatcctg
     4981 ttcgtgaccg ccgtcggcgg tttcctgtcg ccggtgaggg cggcgcttgg cgcggtcgcc
     5041 ttcggcatcg gcgaatcgct gtgggcaggc tatttcccca tcgaatggcg cgacgcatgg
     5101 atgtattttc tcctcgtcgc catgctggtg ctggtcggcg ccggccgcga agaaaacaaa
     5161 gccgtataat ccctagcgca tttgccgccg tgttgacccc tccgccctcg cgcggcatac
     5221 ggggacattc gaaatggtgc tccggccgaa ggaacgaaca gggatgggct ttttcattgc
     5281 cgtctatcta tcgccggtgt tgctcgttct tgtcgtcgtc gcgctgttca tcctggcagg
     5341 catcttgctc gaccggcgga tgggtttcct gcttgcgcta tcgcgcggta tcgaccggct
     5401 gaacgaattc gtcggcaagt cggtgtcgtg gcttatcctc gccgccatcc tggtcagcgc
     5461 cggcaacgcc atcatccgca aggtgttcga catgtcgtcg aatgcgtggc tcgaactgca
     5521 atggtatctg ttcggggctg ccttcctgct ggccgccgcc cacgcgctga agcagaacga
     5581 gcacattcgc atcgacattg tctacggcct gttttcgcgg cgcgtccagc actgggtcga
     5641 cctgttcggc catgtcttct tcctgatgcc gttttccgcg ctgatggtgg tctatctcgt
     5701 tccctatgtg acccgctcgg tggagagcgg cgagatgtcg accaatgccg gcgggctgat
     5761 catctggccg gccaaggcgc tgcttctggc aggcttcctg cttctggcgc tgcaaggcgt
     5821 ttcggagatc gtcaagaaga tcgccgtcat gcgcggcaag acggacgatc ccaacccgtt
     5881 catcccggcg catgaacaag cggaactcga agccaaggcg ctggccggcg aggtgcgggc
     5941 atgatcgagc ttattgccca gaacatggcg ccgatcatgt tctgctcgct gatcgtcttc
     6001 atgctgatcg gctatcccgt cgccttctcg ctggccgcca acggactttt gttcttcctg
     6061 atcggtgttg aactggcgcc ctattccaac ggctcgatca atctttcctg gccgctgctg
     6121 cacgccttgc ccgaccgctt ctatggcggg gtgatggcca acgagacgct gctggcgatc
     6181 ccgttcttca ccttcatggg catcgtgctc gaacgctccg gcatggccga ggatttgcta
     6241 gacacgatcg ggcagttgtt cgggccaatc cgcggcgggc ttgcctatgc cgtcatcatc
     6301 gtcggcgcgc tcttggctgc aaccaccggc gtggtcgcgg cctcggtcat cgccatgggg
     6361 ctgatttcgc tgccgatcat gctgcgctac ggctatgacc gccggctcgc ttccggcgtc
     6421 atcgccgcgt caggcacgct ggcgcagatc atcccgccgt cgctcgttct cattgttctg
     6481 gccgaccagt tgggccgctc ggtgggcgac atgtatgccg gcgcgctcat tccgggcctt
     6541 gtactgaccg gcctctatat gggctatatc ctcatcgtgt cgatcgtccg gccaaagctg
     6601 gtgccggcgc tgccactgga agcgcgaacg ctcggccacg gcgtcttgtc gctgatcgcg
     6661 gcgctgatcg ccaccgcgct ggtttcctat gcggcctacc gctatctggc gccgacgcag
     6721 ggcgagaatg ccgacattct ggccgccacc gtcggcatca tcttcatcta ttgcgtggcg
     6781 ctggccgaca agaccttcaa gttcaacctg atgtcgcggc tggcgcagca ggtggtgatc
     6841 gtgctgatcc caccgctggc gctcatcttt ctcgttttgg gcaccatctt tcttggcatc
     6901 gccacgccta ccgaaggcgg cgccatgggc gcggtcggcg cgctggtgat ggcggcggca
     6961 aagcgccgcc tgtcgctcga cctggtcaag caggcgctgg cagcgaccac gcggctgtca
     7021 tccttcgtgc tattcatcct gatcggcgcg cgggtgtttt cgctgacctt ctacggtgtc
     7081 aacggccatc tttgggtcga acatcttttg gtgtcgctgc cgggcgggga agtcgggttt
     7141 ctcatcttcg tcaacctgct ggtgtttttc ctggccttct tcctcgattt cttcgagctt
     7201 gccttcatca tcgtgccgct gttggcgccg gccgccgaaa aactcggcat cgacctgatc
     7261 tggttcggcg tgcttctcgg catcaacatg cagaccagct tcatgcatcc gcccttcggc
     7321 ttcgcgctgt tctatcttcg ctccgtcgcc gcgcgcgtgc cttatctcga ccgcatcacc
     7381 ggcaagcgga ccgcgccggt gacgaccggg caaatctatt ggggcgccgt gccattcgtg
     7441 tgcatccaga tactgatgat cggccttacc atcgcctttc ccggcatggt catgcactac
     7501 aagggcaagc cggtcgatcc gggcagcatc gagatcaaga tgccggacat gcccggcctg
     7561 ccgccgttgg gcgcgccaca atctggcggc gcaggtgcta cgcccgacct ttccgcgccg
     7621 ccgaatttcg gggcgcccgc accggcgaaa cccgccacgc ccgccgtgcc ggacctgtcg
     7681 cagccgccca gcttcaactg agcataaaaa agccccgaag gcaggcctcc ggggcttttt
     7741 gtcgtagggc aggaaacgcg gctacagctt gccggcgcgc tgctggatca tcatgaaggt
     7801 gtcgaaatta tattcggcga cctgctgcca cagataatgt tccttgcgga aggcgacgat
     7861 cgattcccaa atcttcttga aggtcggatt ggtgttcatc atctccgcat agacctcgtt
     7921 ggacttgtcg aagcaggcca gcatgatctc ctggctgaac gggcgcagca gcgcgccgtt
     7981 ggcgaccagc gacttcaccg ccttggaatt gagataatcg tatttctgca acatgtcggc
     8041 gtcggtcgcg cgcgccgccg tctgcaacag cgacttgtag gtcggcgaaa gctcgtcgta
     8101 cttcgccttg ttgaacatca gatggacggt cggtccgcct tcccaccagc cgggataata
     8161 ataatagggc gcgaccttct ggaagccgag cttttcgtcg tcataggggc cgacccattc
     8221 ggcggcgtcg atggtgccct tttccagcgc cggatagatg tcgccgccgg caagctgctg
     8281 cggcacgacg cccagccttt ccaccacctt gccggcaaag ccgccaatgc gcatcttaag
     8341 gccggacaga tcggcaacgg tgttgatttc cttgcggaac cagccaccca tctgcgtgcc
     8401 ggtgttgccg cccggaaaac cgaccagccc ctgcgtggcg aggaattcat tataaagatc
     8461 gttgccgccg ccatggtaga gccaggcgtt catgccgcgc gcgttgagcg cgaaggggac
     8521 agccgcgccc agcgcccaga ccgggtcctt gccccagtaa taataaccga ccgtgtggca
     8581 cgcctcgacg gttccagcgg tggtggcgtc ggccgcctga aggcccggaa ccagttcccc
     8641 ggcggcaaat acctgcacct ggaaattgcc atccgtcgct tccgaaagca tcttcgagaa
     8701 ggtttcggcc ccgccataga tggtatcgag cgatttcggg aaggacgacg tcagccgcca
     8761 tgtgaccttg ggattggact gggcgatggc aggcgcggca agtgtcgcaa ccgtggcggc
     8821 agcgccggcg ccggtcacgc cggcctttct gatgaatgaa cggcgatcca tgaattcctc
     8881 cctaggcgaa tcaaaaaagc gggccgtcgc tgtaattccc tgaatttccg gacggttcgg
     8941 gcagacaata cacgccgaag aagtcgagtc cagcatggcg gcagtttctt caaaggcaga
     9001 aatcaggctg tgctcatatg cgcggcacaa ggcgaaactt gtcgtggaaa tgtcctgcca
     9061 aatcgcctat tttgaatgaa ggcaagtcac gcccaaccgg attcaggtag ccgcatgtcc
     9121 cagccgctcg ttgccgtctc gaccgatgta aagcagttcg agaactacac atggcacgcc
     9181 gcgccccagc aatatctgga agcggcgatt tcgacggcgg gcgtatttcc gctcttggtg
     9241 ccatcgttcg gcgaccggct tgaccttgac gaactgctct cgcgcgtcga cggcgtgatg
     9301 atgaccggct cgaaatccaa cgtctatcca gccctttacg gcggcgacgc cagtgaggcg
     9361 aacggtccct acgaccacgc ccgcgacgcc accactttgc cgctgatccg cagtgctatc
     9421 gagcgcggcg tgccgctgct ggccatctgc cgcggcatcc aggaaatgaa cgtcgcgcta
     9481 ggcggcacgc tgggcacgga aatccaggaa cgcgaaggcg cgctggacca ccgcgcgcca
     9541 ccaagcgaca atcaggacga gcgcttcgca attcgccaga acgtatcgat caaggccggc
     9601 agttgccttg cgggcgtgtt cggcgcgggc gacatcatag tcaattccgt gcatcgtcag
     9661 ggcgtcgacc ggcttgggcc gaagctgcaa gtcgaagcgg tggccgagga cggcacggtc
     9721 gaggcggtat ccgcccgcga ttcccgcgct tttgccgtcg gcgtccagtg gcacccggaa
     9781 tattgggcga agtccgacga cgcctcgcag cgcatcttcc aggcatttgg cgaagcggtg
     9841 cgccaacatg cggccggcaa gatcgcggcc cgcgcggcgg ctgaataaag ccgccacacc
     9901 agcaaacaag ccggttattt cggcttgacg ccgaccgttt caggtaccgg cgtcagcgcg
     9961 ttgccgtcgc ggaaccacgc gatcagatta tcgacgcaca aatccgccat tgcgcgccgc
    10021 gtatgtatcg aagccgaggc gatatgcggc aatagcagcg tgttgggcgc gtcgagcagc
    10081 gactgcggca cattcggctc gtcggcgaag acatccagtc cggcggcggc aatcgtgccg
    10141 tcggccaagg ccttggccag cgccgcctca tccaccgtcg aaccgcggcc gatattgacg
    10201 agcacgcctt cagcgccaag agccgacaga atttgcgcgt tgaccgcgtt ttcggtggcg
    10261 gccccacccg gcgcgatgca aatcaacgta tcgaccgcct cggccagatc cttcagcgtc
    10321 gcgtgatagg cgtaggccac gccctcgaca ggccgccggt tgtgataggc gatcgccagg
    10381 ccgaacgctt ccagccgccg ggcgaccgcg atgccgatgc ggccgaggcc gaaaatgccg
    10441 acggtgcggc cacgcagggt cagccggctt tgcggatagt tgccgttgcg cttccagtcg
    10501 ccttcacgca gccatcgctc tgccggcgaa aactcacgga tggtcgaaat cagcaagccg
    10561 atcgcggtat cggcgacctc ttcggtcagc acatccggcg tgttcgtcac catgatgccc
    10621 tgccgtccgg cataggcggc atcgatgccg tcatagccga cgccgaaatt ggcgatgatc
    10681 tcggctttcg gcagcgcgcg caggaaatcc ggcccgacaa acgagccgcc tatggcgggc
    10741 gcggcagcaa ccccgcgcac ggtgtcgcgc agttcgggcg tcaccaatgc cggatcgatg
    10801 cggtcgatcg agaccagctt gaacgtatcg cttatccgcg ccaccgcatg ctcatgcaac
    10861 ttgccgggga cgaggatggt gatggagcgt aggtcttcgg ccatgctgat gctttcctgg
    10921 caaggagttc aggtgcgttc gaccggcctg ccggttgatt ggcgcacatg cagttcgggc
    10981 ttgatcaatt cctgcggggt gcgcacgctc tctccattga gcttgtcgag cagcgcgctg
    11041 gcggcgcgcc gcccgacctc gcgctggccg ttccacaccg tggtcagggc aggcgtcgcg
    11101 atggacgcct cttccagatc gtcatagccg gtgacg
//
LOCUS       DASYCS010000196        19536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_917448,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000196 DASYCS010000000
VERSION     DASYCS010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 19536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19536
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917448"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1118)
                     /locus_tag="VGM46_06300"
     CDS             complement(<1..1118)
                     /locus_tag="VGM46_06300"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006204811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY4192239.1"
                     /translation="MFEVTVENSFDFLSQEYRALFDKSAATAFQHPNWLSRLYGKLLT
                     ANMATPLVIVVRHQADGALAMVLPLVRRRYAALRVIEFADLRVSDYVSPVTDPATFEA
                     IAADAACVAKLRGMLKPYDLLRIGKLHDGSLPLEKLLGIRNRENMGMSAYATPLEDSY
                     PVWRQKRIKQSYCKELDKKSRQLHRRGEVRFECVTEPDDLLKAFEAMRAYRGERFKDR
                     DGVGDLLQQPAYYDFYTGIAKAGRDGSARTYAMWLDGRPIAVALGLAHRGALLVILGG
                     FDFGFKNMSIGSLMFEQVARDCIERGERHLDFTIGDEPYKLTFGSEPSAMWQISRSGS
                     PLGFAAGLVVERLPAAKKLARRMFHGAKDAQPAARAAPA"
     gene            1299..2774
                     /locus_tag="VGM46_06305"
     CDS             1299..2774
                     /locus_tag="VGM46_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006204810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192240.1"
                     /translation="MTAVEVIVPHPAPRLWQRVLVQRLQAAGHEVSVTRQAGAARWPL
                     AMTAAMRFERRLLRRHGHGLASVTAEIDEISPSRAPELRLDLSGIAARTDVPTLALTF
                     DGDGWDGMAVTALVEGRLPGIEAVLDGQKVVGQAFPMVDKRESVALGADDVLARAITL
                     AASLVTSFGAGLSTTGATPARRANTATDGLRFAASYVCHALPRLGREALRRARFRNAH
                     WRVGYRFIDGPGIAETGCIGNGWRQLPDEGDHFYADPFPFEWQGRSFIFVEDYPHVTK
                     KAVISVVEFNEDGSPKPPKLVLEEPFHLSYPQVFAWNGQVWMLPEGSAGGRLTLYRAM
                     EFPHRWAAATTLLEGEVSDATLLEHGGRWWLFATVRDGFGSTSDTMAVFWAPSPDGPW
                     TPHARNPILIDRRAARPGGAFIRAGGKLLLPVQDGTRGYGGGLGLSEVLQLDTEQVRL
                     SPPVAVREAGDWPYPQIHTLNRAGRLEVIDGIAAIRKRSRR"
     gene            complement(2763..3863)
                     /locus_tag="VGM46_06310"
     CDS             complement(2763..3863)
                     /locus_tag="VGM46_06310"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006204809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY4192241.1"
                     /translation="MKIVQVQTQAEAAGAQRVSDMVGEGLRGRGHDVRTVFMYRKTDA
                     YDRDEHADFVLREPPRGLAGQLKASLGLMRYLRRARPEAVISYQHYGNIFGTLGARLA
                     GARHIVANQSGAPQQSGVMGLLSRVDRLMGVAGVYQSNVVNSAWTEAQFDDYPDAYRR
                     RIRRIDHGVGGPPQAYDKAEARAAFGLPQDRFVAISTGRLSVQKNHIALAGVLREIPD
                     LHVAIAGAGPERETLTAFASEHGVAARLHLVGEVAPARIFEFLATGDAYVFPSTNETF
                     GLAVAEAAIAGLPVIANGLAVLREVLTGDDGEPAALFTEDADAKSFSAALSRIMERPD
                     LADKLAAGGRSLARRYAPDRMCIGYENLLLAP"
     gene            complement(3860..5263)
                     /locus_tag="VGM46_06315"
     CDS             complement(3860..5263)
                     /locus_tag="VGM46_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006204808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEY4192242.1"
                     /translation="MKDRETTVRPTPAAAGAAPRMSIAGLARSGAIAGVIKLASAGLS
                     FLMFVVIAMVTDERQFGLYSATYAGASLVSFFASVGQQSTVLRFWPQYAGVDDLSSAH
                     GLMRRAIIVALAGVTVSSVLVVAVGFVPYAGQGTPEWLPLCLSAAILSLALAWSEFSS
                     GAFRAKGALVSGLLPRDIIWRVATIAAMAVLWYLHREITAVTATLVTAGLLILAILPQ
                     TAALLRETFRAQRNPLSAQQKAEFNSVTLGLWGVTSLPPALGQVSTLLVAAILGPEIA
                     GAVFVADRTTRLVVLALTGINQALAPEISGAFYAGDKNHVQRTTSLTALGSSAVAVAV
                     LAIFWIFGKEILGIFDPAYATPTMHAVLIVFGTGATVAAACGPMELVMQLTGLQHSLL
                     KLLIVVNVVGLAVTALMTYLFGAVGAAVGIAGTVVTWNIIGVTIACRSIGINPSLVGL
                     FTGRRPSRLGPASHSAS"
     gene            5599..7761
                     /locus_tag="VGM46_06320"
     CDS             5599..7761
                     /locus_tag="VGM46_06320"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GumC family protein"
                     /protein_id="HEY4192243.1"
                     /translation="MSQAIADDRDLPPLFDIGAVLKTLWDRRALIAIITVTAVLAALL
                     YVAVTRPVYTANASILVDPRDSRATSFDNVLPGIGADSAAIASQVAVIDSTDLLKAVF
                     DTEKLQDDPEFNGRGLLSRLLSPLRSSGPAGDDAAFASFRDKVSVEREGLTYVIDIGF
                     KSQDPKKAAQIVKAIVQNYKASLSGEKETANSQANTLLTDKIGGLQKAVSDAERAIQD
                     FKFEHRIFDAEAGGTLQSQIDQFSAQLVTAQDQADRAQSRYDQAVAAGTTPEGLSRLS
                     AILSSGTAEKLRQDYNDRAASLANAQTDLGPKHPTIARLSAELARIRSLMGREAQRIT
                     QELKANRDIAAQNVKQLQAKLDSLRFNSNDSNLAQVQLRQLQRKADAARAVLDDFLKR
                     SQETLHMQGMQISQVREISEAVPPIQPTWPKPKLLLPVSAVLGLMLGCALALMLGPVQ
                     GGAAEARRRSGRAGENPNGAPDPSPRPRRPIPANLGAYAVPLAPGGNVRDGVHAIRRK
                     IEAFDSAPFLLSMQQLLVRITGHLNEHPKPFVLLLSSVQQGGERNLAAALIGLGLQHI
                     KERVLVIEIAGQPYPYGMVQRSRKPVVDPASGLPTVVVDALVMTPNGPADNAGAVMQI
                     LAEQQGAFGFALVIARPLDDPAYSFALAARADLAIYALTPAQQRAGAISWIGQRLAPK
                     GAADGLARTATVVIEQRMERMQPRAATADGRRKTAARG"
     gene            7766..8896
                     /locus_tag="VGM46_06325"
     CDS             7766..8896
                     /locus_tag="VGM46_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015342576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEY4192244.1"
                     /translation="MTRQQSPAARSTLTRRQAVGGVAALLAAPLLPSPGMADAAPVVP
                     TRGFNLPGWVDREDGTAPAKATLEKLRGLGFETIRLPVAADPLLSGASAQATMLGRID
                     AAIGEALAGGFAVIVDLHPGEKFAAALRNDPAQGGKQAVAAWDALAGVVAGQPAGKVY
                     AELLNEPPMQPAAWLELRDRLAETVRAKCPRHTIIWGTAPVQGMWELADAPLLADDNA
                     IVAVHYYTPMGFTHQCENWDGSPLGRLANLPFPATKDGPQAASLFRKFKETGDTDATA
                     LLNQEFTGPWTVEHIASDFARLGEWSRNNRCPAIVDEFGVLDFCVDAPSRGNWVRAVR
                     QAAEANGIGWAYWELDQGFGFIKDRTSTEGFDSAMIDALVKG"
     gene            8902..10491
                     /locus_tag="VGM46_06330"
     CDS             8902..10491
                     /locus_tag="VGM46_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006204805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192245.1"
                     /translation="MSSAVSHPGKAPARRMPQSGAEAALHIVVALLLIGAAVSGFAVS
                     TVFGAVASLVLTLCVATALPAAMPVVIVCAFLFQNMIVAWFTPYIPDNNAFDALRGAN
                     FVVLMTAYAAFLAASLQVRLRAVTQARPWLLWGIALAGIITFYMLLGAVRGGPKDSII
                     YFRNTITPLACFHIGIVAASLYRIDLRKSLLWLGASAIVYGYCELIFTMDFLGLFNGD
                     LYIQRAIRRQIETGVWEKALQQTGFVLRGLQDVMTTTFFNTPLFADLFPPVFRIGGPN
                     FHPISYAYALGILSVWLLFKGRWLLPLAALPLLLVIGSKGAMFMVVVALAVRLLQGPL
                     GMRLTLLGVLAVAAAWIIAAIGYGATHGDYHVLGLIAGLRGFLGNPLGQGLGIGGNLS
                     STSFNLDWDRAQAQGVASVPVESAVGVMLYQMGVGSIVFFGFLAALVASARRLLLKTG
                     NAEFLFAFVAIVTISTNAVLQEEAFYSPLALGFCLLLVGTAFGTYWREESLVARSAAA
                     SRTIPQGHAALRAGSAKGVRP"
     gene            complement(10562..11512)
                     /locus_tag="VGM46_06335"
     CDS             complement(10562..11512)
                     /locus_tag="VGM46_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEY4192246.1"
                     /translation="MAAHNSSRTVIYAALAGNMAIAITKFAAAFFTGSSAMLSEGVHS
                     LVDTGNGGLLLYGLHRASRPADRTHPLGHGRELYFWSFIVALLVFALGAGVSFYEGIV
                     HIIAPEPVVNAKVNYIVLGLSVLFEGSSWWIALKEFRQAKGRLGWFKAVRVSKDPSVY
                     TILFEDSAALLGLAVAFAGIFAAQMLNMPELDGVASLGIAVILGSTAIFLARESKSLL
                     MGEPASPATQQKILEIVQQDPAVQRANGVLTVHLGPDQIVAGVSVEFEDHLAAPEIEA
                     CVERLEARLKADVPKVTSLFVKPQTAGTWQQRRDVIEQAD"
     gene            11648..11776
                     /locus_tag="VGM46_06340"
     CDS             11648..11776
                     /locus_tag="VGM46_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015314442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192247.1"
                     /translation="MAKGAMKAGKEARKPKKDAKKLVPAAPALKAPPVKATRLKEK"
     gene            11963..12277
                     /gene="sugE"
                     /locus_tag="VGM46_06345"
     CDS             11963..12277
                     /gene="sugE"
                     /locus_tag="VGM46_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003559670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HEY4192248.1"
                     /translation="MAWTILFFAGLFEVGWAIGLKYTEGFSRPLPTILTVVSMIVSLA
                     LLGLALKTLPVGTAYAVWTGIGTVGTALLGVWLLGEPATAIRLACIALIVCGIAGLKL
                     AA"
     gene            complement(12510..12938)
                     /locus_tag="VGM46_06350"
     CDS             complement(12510..12938)
                     /locus_tag="VGM46_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013891840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6481 family protein"
                     /protein_id="HEY4192249.1"
                     /translation="MAIYREKDVFERRNAANEAKKALLERFKAKPAADDPAVLARQAE
                     RKAILEAREKREAEKATLKQERLAREAADKAEREAAAEAARIAAEEAAQAEAKLRDAE
                     ETERIARLLADEAERKAKRDARYAARKQRTGRTPPGFSAR"
     gene            complement(13151..14410)
                     /locus_tag="VGM46_06355"
     CDS             complement(13151..14410)
                     /locus_tag="VGM46_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006325831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEY4192250.1"
                     /translation="MNETGITISEPIIRLGATTITLGQALSFGAILLLLALAWLAVAL
                     WRSSHARAMAAAEAADHAREAEARMAGILQSQAEMQGRMGAIAEVFGARQAELTQSIG
                     QRLDAMTGRLGQTMTEQTKSTHESLSKLQERLAIIDTAQGNIQSLAGQVVQLQAILSN
                     KQTRGAFGQSRMEAIVADGLPMGSYEFQATLSNGSRPDCLVKMPNGAPSLAIDAKFPL
                     EAWNAIRAAESPELQKVAAQAFRRDIEVHVRAISEKYLIQGETQDTAFMFVPSESVFA
                     EIHENFEGTVHKAHRARIVIVSPSLLMLSIQVIQAILKDARMREQAHLIQGEVVRLME
                     DVARVDERVRKLQTHFGQSAKDIEDILVSTSKVTKRGQKIEALELRAPGQDAGGQAGT
                     ADGEGQARRVADSKTGQLRLRVVEGDD"
     gene            14513..15043
                     /gene="def"
                     /locus_tag="VGM46_06360"
     CDS             14513..15043
                     /gene="def"
                     /locus_tag="VGM46_06360"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEY4192251.1"
                     /translation="MSIKPLIILPDPLLRQLSKPLERVDAQVARLADDMLETMYDAPG
                     IGLAAIQIGEPLRMLVVDLAKEDEAPAPHVFINPEILQRSDERSVYEEGCLSIPDYYA
                     EVERPASVRVKYLDRDGNSQEIEAEGLMATCLQHEIDHLDGVLFVDHISKLKRDIVVR
                     KFKKLAKDRAPGKLVG"
     gene            15052..15987
                     /gene="fmt"
                     /locus_tag="VGM46_06365"
     CDS             15052..15987
                     /gene="fmt"
                     /locus_tag="VGM46_06365"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013891848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEY4192252.1"
                     /translation="MPLRLVFMGTPDFSVPTLRALAGAGHDIAAVYTQPPRAGGRRGL
                     ELTPSPVQGEAERLGVEVRAPASLKGEAEQQAFRALGADVAVVIAYGLLLPQAILDAP
                     RLACLNGHASLLPRWRGAAPIQRAIMAGDAETGMMVMKMEAGLDTGPVALVDKVAIAA
                     DMTAGELHDRLMDAGARLMVEAVAALEAGRLAFSPQAAEGVTYAQKIDKSETRVDWTL
                     SATEVHNHIRGLSPFPGAWCEVEIGGRRERLKLLRSAMAEGLGEPGGILDDRLTVACG
                     AGAVRLVEVQRAGGKPVAASEFLRGTRLEKGMKLS"
     gene            15984..16490
                     /locus_tag="VGM46_06370"
     CDS             15984..16490
                     /locus_tag="VGM46_06370"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEY4192253.1"
                     /translation="MSMMSIRAATPRDRDAIRLVEEHAFGQQAEAGLVDALIEGGDAI
                     VELVAVEDGQVVGHILFSRLYVQQGGRKYPAVALAPLAVEHSFHGTGIGGALIREAHI
                     RLKAAGETLSIVLGDPAYYGRFGYSHERAEKFESAYQGEALQALSWGEAPQAGLLVYA
                     SAFEALAA"
     gene            16506..17261
                     /gene="truA"
                     /locus_tag="VGM46_06375"
     CDS             16506..17261
                     /gene="truA"
                     /locus_tag="VGM46_06375"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006325815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEY4192254.1"
                     /translation="MARFRLDIEYDGGAYAGWQRQADQPSVQQAIEQAIARFSGDAVS
                     LRGAGRTDAGVHASGQVAHADLTKDWTGDKVRDAVNAHLQMAGERVSILKATPVADDF
                     DARFSALGRHYLYRIVNRRSPPALEMGKVWWVPKPLDAQAMHEAAKVLLGRHDFTTFR
                     STQCQANSPVRTLARLDVTREGDVIEIRASARSFLHNQVRSITGSLKRVGDGGWTADD
                     LKAALEARDRAACGQVAPPDGLYLTKVDYPEAE"
     gene            complement(17368..17961)
                     /locus_tag="VGM46_06380"
     CDS             complement(17368..17961)
                     /locus_tag="VGM46_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEY4192255.1"
                     /translation="MLSADETQASLAGAWKLMLGKADGLRLLDLSADGFWNSFFAIVV
                     ALPALIVGWVSVVNEVADPAAFAGRFDTLLRLAAVDLGAWVLPLVGLAFVAPHAGIGG
                     RFVAYVVASNWASAIVAWLMLPPALIRLVMPDPDSIANFFSFFCFLLSMVLTWRMTNA
                     AIGKGAAVGTAVFAGMFVVSLLVLFALQALLGISFPG"
     gene            complement(17977..19170)
                     /gene="dapE"
                     /locus_tag="VGM46_06385"
     CDS             complement(17977..19170)
                     /gene="dapE"
                     /locus_tag="VGM46_06385"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015314432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEY4192256.1"
                     /translation="MTLPTDPAENLAILIRCPSVTPAEGGALAALQQMLEPLGFAVER
                     PSFSDDGTPTIENLYARRSGNGPHLMFAGHTDVVPTGDEAAWTHPPFSAAIANGEMYG
                     RGAVDMKGGIACFIAAVARHVEKHGGPKGSISLLITGDEEGPAINGTTKLLDWAAAKG
                     ETWDAAIVGEPTNPDALGDAIKIGRRGSLSGTVTVNGRQGHVAYPQLADNPVRGLMSL
                     VDALLAPAFDKGTKDFQPTNLEVTSIDVGNPATNVIPAKATATFNIRFNDTWSAETMQ
                     AEIHNRLDQAARRRKYRAGRKEPVDYELVWRDRPSHVFLTRDDRLIETLSGSIGAVIG
                     KEPALSTSGGTSDARFIKDYCPVVEFGLVGQTMHMVDERVALADLEKLTQIYGRFIED
                     WFEKG"
     gene            complement(19282..>19536)
                     /locus_tag="VGM46_06390"
     CDS             complement(19282..>19536)
                     /locus_tag="VGM46_06390"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY4192257.1"
                     /translation="FVILLPNTSEIEATYLAKRSIDDISEPFDLGVATSVRIGLSIGS
                     AFAPSDGSDPEVLLSCSDNALYEAKRTGKGRYRAHVRIVR"
BASE COUNT         3430 a         6234 c         6382 g         3490 t
ORIGIN      
        1 gccggcgcgg cgcgggcagc gggctgtgcg tcttttgcgc cgtgaaacat ccggcgcgcc
       61 agcttcttcg ctgccggcag tctttccacc acaaggccgg cggcaaagcc gagcgggctg
      121 cccgagcgcg agatctgcca catggccgac ggttcggagc cgaaggtcag cttgtagggt
      181 tcatcgccga tggtgaaatc gaggtggcgc tcgccgcgct cgatgcagtc gcgcgccacc
      241 tgctcgaaca tcaggctgcc gatcgacatg ttcttgaaac cgaaatcgaa gccgccaaga
      301 atgaccagca gcgcgccgcg atgcgccagg ccaagcgcaa cagcgatagg ccgtccatcc
      361 agccacatcg cataggtgcg ggccgagccg tcacgcccgg ccttggcgat accggtatag
      421 aaatcgtagt aggcgggctg ttgcagaaga tcgccgaccc cgtcgcggtc cttgaaacgt
      481 tccccgcgat aggcgcgcat cgcctcgaag gccttcagca aatcatccgg ctcggtgacg
      541 cactcgaagc ggacctcgcc acggcgatgc aactggcggg acttcttgtc cagttccttg
      601 caataggatt gctttatccg cttctgccgc cataccggat aggaatcttc cagcggcgtc
      661 gcataggcgc tcatccccat gttttcacgg ttgcggatgc ccaacagctt ttcgagcggc
      721 aacgaaccgt catgcagctt gccgatccgc agcagatcat acggcttcag catgccgcgc
      781 agctttgcca cgcaggccgc atcggcggca atggcctcga aggtcgccgg atcggtaacg
      841 ggcgatacgt aatcggatac gcgcaggtcg gcgaattcga tgacgcgcaa cgccgcatag
      901 cgccgccgga ccagcggcag caccatggcg agtgcgccat cggcctgatg gcgcacgacg
      961 atgacgagcg gcgtcgccat attcgccgta agcagctttc catagaggcg cgaaagccaa
     1021 ttgggatgct ggaacgccgt ggcggccgat ttgtcgaaca atgcgcgata ttcctgcgac
     1081 aggaaatcga acgagttctc aacggtgact tcgaacatgg ggaccattta agctttgcgg
     1141 gaagccactt ttcagccgat ctgtctcccg gcgcgtgacg ggagtcaatt gatagttaca
     1201 atattggttt atggccgatg gagtagcgcg ggccggctca aggattgata gggcggcgcg
     1261 ttcgtactat ccgggcaggg cgcttgggag aaatatcgtt gacggcagta gaggtgattg
     1321 tcccgcatcc cgcgccgagg ctctggcagc gcgtgctggt gcagcgattg caggcggccg
     1381 ggcacgaggt gtcggtcacg cgccaggccg gcgctgcccg ttggccgttg gcgatgacgg
     1441 cggcgatgcg ttttgagcgg cgtcttctgc gtcgccacgg ccatggcctt gcttcggtga
     1501 cagctgaaat tgacgagata tccccgtcac gcgcgccgga gttgcggctg gacctttccg
     1561 gtattgccgc acggaccgac gtgccgacgc ttgccttgac gttcgacggc gatggatggg
     1621 acggcatggc ggtgaccgcg cttgtcgagg gtcgactgcc gggcatcgaa gctgtccttg
     1681 acggtcaaaa agtcgtcggc caggcgtttc cgatggtcga caagcgggaa tcggtggcgc
     1741 tgggcgcgga cgatgtgctg gcgcgcgcca tcacgctggc cgcctcgctg gttaccagct
     1801 ttggcgcggg tctttcaact acgggcgcaa cgccggctcg gcgtgccaac acggccacgg
     1861 atggcctgcg ctttgcagcg tcctatgttt gccacgcttt gccgcgcctt ggccgcgaag
     1921 ccttgcgccg cgcgcgcttt cgcaacgccc attggcgggt cggctaccgc ttcatcgatg
     1981 gccccggcat cgccgagacc ggctgcatcg gcaatggctg gcggcaattg cccgacgagg
     2041 gcgaccactt ctacgccgat ccgtttccct tcgaatggca gggccggtcc tttatcttcg
     2101 tcgaagacta tccccacgtt acgaaaaaag ccgtcatctc ggtcgtggaa ttcaacgaag
     2161 acggctcgcc aaagccaccc aaactggttc tggaagaacc gttccacctt tcctacccgc
     2221 aagtctttgc ctggaacggg caggtgtgga tgctgccgga aggcagcgcc ggtggccggc
     2281 tgacgcttta ccgcgcgatg gaatttccgc accgctgggc ggcggcaacg acgttgctgg
     2341 aaggcgaggt ttcggatgcg acgctgctcg aacatggcgg gcgctggtgg cttttcgcga
     2401 cggtgcgcga cggctttggc agtacttcgg atacgatggc ggtgttctgg gcgccgtcgc
     2461 ccgatggccc gtggacgccg cacgcaagaa acccgatcct gatcgaccgc cgcgcggccc
     2521 gtcccggcgg cgctttcatc cgtgccggcg gcaagcttct ccttccggtg caggacggca
     2581 cgcgcggcta tggcggcggg cttggcctat cggaagttct ccagctggac acagaacaag
     2641 tgcgcctgtc gccgcctgtc gccgtccggg aggcgggcga ctggccctat ccgcaaatcc
     2701 acacgctcaa ccgggccggc cggctggagg tcatcgatgg catcgccgcc atccgcaaac
     2761 gctcacggcg ctagcagcag gttttcgtaa ccgatgcaca tcctgtcggg agcatagcgg
     2821 cgcgccaggc tgcgtccgcc agccgcaagc ttgtcggcaa ggtcgggccg ttccatgatg
     2881 cgggaaaggg cggcggagaa gctttttgca tcggcatctt cggtaaacag tgccgccggc
     2941 tcgccatcgt cgccggtcag cacctcgcgc agcacggcaa gcccgttggc gatcaccggc
     3001 aggccggcaa tggccgcttc cgccaccgca aggccgaagg tctcgttggt tgaaggaaaa
     3061 acataggcgt cgccggtggc gaggaattcg aagatccgcg ccggcgccac ctcgccgacg
     3121 agatgaagcc gcgccgccac gccgtgctcg cttgcaaagg cggtcagcgt ctcgcgctcc
     3181 ggccccgcac ccgcaatggc gacatgcagg tccggtattt cgcgcaggac cccggccagg
     3241 gcgatatggt tcttctggac gctgagccgg cccgtgctga tcgccacgaa acgatcctgc
     3301 ggcaggccga atgcggcgcg cgcttcggct ttgtcatagg cttgaggcgg cccgccaacg
     3361 ccgtggtcga tgcggcggat gcgccttcta taggcatcgg gatagtcgtc gaactgggct
     3421 tcggtccatg ccgagttcac cacgttcgat tgatagacgc cggccacgcc catcagccgg
     3481 tcgacccggc tgagcaggcc catgacgccg ctctgctgcg gcgcgccgct ctggttggcg
     3541 acgatatggc gggcgccggc aaggcgggcg cccagcgtgc cgaaaatatt gccgtaatgc
     3601 tggtaggaaa tcaccgcttc cgggcgcgcc cggcgcagat agcgcatcag cccgagcgaa
     3661 gctttcaact ggcctgcaag cccgcgcggc ggctcgcgca gcacgaagtc ggcatgttcg
     3721 tcgcggtcgt aggcatcggt cttgcggtac atgaacacgg tgcgcacatc gtggccacgc
     3781 ccgcgcaagc cctcgccgac catatcggac acgcgctgcg cgccggcggc ctcggcctgg
     3841 gtctgcacct ggacgatctt catgaggcgc tgtgcgaagc cgggccaagc cgcgacgggc
     3901 ggcgccccgt gaacaatccg acgagcgacg gattgatgcc gatggagcgg caggcgatcg
     3961 tcacgccgat gatgttccag gtaacgaccg tgccggcgat gccgaccgcc gcaccgaccg
     4021 cgccgaacag ataggtcatc agcgctgtga cggcgaggcc gaccacgttg acgacgatca
     4081 aaagcttgag cagcgaatgt tgcaggccgg tcagctgcat caccagttcc atcgggccgc
     4141 aggcggcggc cacggtagca ccggtgccga agacgatcag caccgcatgc atggtcggcg
     4201 tcgcataggc ggggtcgaat atgccgagaa tttccttgcc gaaaatccag aagatggcga
     4261 gcaccgcgac ggcgactgcg ctgctgccca gagccgtcag gctggtggtg cgctgcacat
     4321 ggttcttgtc gccggcgtag aacgcccccg atatttcggg cgccagcgcc tgattaatgc
     4381 cggtaagcgc cagcaccacc aggcgcgtgg tgcggtccgc cacgaacacc gcaccggcga
     4441 tttccggccc aaggatggcg gccacgagaa gcgtgctgac ctgcccaagc gccggtggca
     4501 gcgaggtgac gccccaaagg ccgagcgtga cgctgttgaa ctcggctttc tgttgcgccg
     4561 acaatggatt gcgctgcgcg cggaacgttt cgcgcagcag cgccgccgtc tgcggcagga
     4621 tggcgaggat cagcagcccg gccgtaacca gcgtggcggt aaccgccgtg atttccctgt
     4681 gcagatacca gagcaccgcc atggcggcga tggtggcgac ccgccagata atgtcgcgcg
     4741 gcaaaagccc ggagacgagc gcgcccttgg ctcgaaacgc gccggaggag aattccgacc
     4801 aggcaagcgc cagcgaaaga atggccgccg acaggcatag cggcagccat tcgggcgtgc
     4861 cttggccggc atagggaaca aagccgacgg cgaccaccag aacgctcgaa accgtgacgc
     4921 cggcaagcgc gacgatgatg gcgcgccgca tcagcccatg cgccgaagac aggtcgtcga
     4981 cgcccgcata ttgcggccag aaccgcagca ccgtgctctg ctggccgacg ctggcgaaga
     5041 aggaaacgag gctcgccccg gcataggtcg cgctatagag gccgaactgg cgctcgtcgg
     5101 tgaccatggc gatgacgacg aacatcagga acgacaggcc ggcgctggca agcttgataa
     5161 caccggcgat cgcgccgctg cgcgccagcc cggcgatcga catgcgcggg gccgcgccgg
     5221 cggcggcggg cgtcggtcga acggtggttt cgcggtcttt catcgatgcg gcgccatggg
     5281 ctctgcaaaa ttcacgaagt gccggcgggt tgcttcggtc cggcattcgc cgccggctgc
     5341 ggcaaacctg ccctttaacg tgcccaccca tagtccaaac agcccgtgct gcaccacaag
     5401 gatcgttggc gatggttacc gcgccggcaa gggtggcccg tttcgcagcc ggttcggcgc
     5461 atactatttg gccttcaggc gtcgggccgc gttaaattcc cggtaacctt aacggactgc
     5521 taacgttgat cgaagatggt gattccccag atcaggcatg catgttgacg aacatgcgcc
     5581 tttgaaagga acagctcgat gagccaagcc atcgcagacg atcgcgacct tccgccgttg
     5641 ttcgatattg gcgcggttct gaagacgcta tgggaccgcc gcgcgctcat cgcgattatc
     5701 accgtcacgg ctgtgctggc ggcgctgctc tatgtcgccg tcacgcggcc ggtttatacc
     5761 gccaatgcct ccattctggt cgatccgcgc gattcgcgcg ccaccagctt cgacaatgtg
     5821 ctgccgggca tcggcgccga cagcgccgcc atcgccagcc aggtggcggt catagattcc
     5881 accgatctcc tcaaggcggt tttcgatacg gaaaagcttc aggacgatcc tgaatttaac
     5941 ggtcgcggcc tgctttcgcg gctcttgtcg cctctccgct ccagcggtcc cgccggcgac
     6001 gatgccgcct tcgccagttt ccgcgacaag gtttcggtcg agcgcgaagg gctgacctat
     6061 gtcatcgaca tcggcttcaa gtcgcaggac cccaagaagg cggcccaaat cgtcaaggcc
     6121 atcgtccaga actacaaggc cagcctttcg ggcgaaaagg aaaccgccaa cagccaagcc
     6181 aatacgctgc tcaccgacaa gatcggcgga ttgcagaagg ccgtcagcga tgccgagcgc
     6241 gccatccagg acttcaagtt cgaacaccgc atcttcgatg ccgaggcagg cggcaccctg
     6301 caatcgcaga tcgaccagtt cagcgcgcag ctcgtcacag cacaagacca ggccgaccgg
     6361 gcgcagagcc gttacgacca ggccgtcgcg gctggcacta cgccggaagg gttgagccgt
     6421 ctttccgcta tcttgagttc cggcacggcc gaaaaactcc gccaggacta taatgatcgc
     6481 gccgcgagcc ttgccaacgc gcagaccgat cttgggccga agcatccgac catcgcccgg
     6541 ctttcggccg aacttgccag gatacgcagc ctgatgggcc gggaagccca gcgcatcacg
     6601 caggaactga aggccaaccg cgacatcgcc gcgcaaaacg tcaagcaatt gcaggccaag
     6661 ctcgacagcc tgcgcttcaa ttccaacgat tccaacctgg cgcaggtgca gttgcggcag
     6721 cttcagcgca aggccgacgc ggcgcgcgcg gtgctggatg atttcctgaa gcgctcgcaa
     6781 gagacgttgc acatgcaggg catgcagatt tcgcaggtgc gcgagatcag cgaggccgta
     6841 ccgccgatcc agccgacatg gccgaagccg aagctgctgt tgccggttag cgccgtgctg
     6901 ggcctgatgc tgggctgcgc gctggcgctg atgcttggac cggtgcaggg cggcgcagcc
     6961 gaagcgcgcc gccgcagtgg ccgggcgggc gaaaacccca atggcgcgcc cgatccgtcg
     7021 ccgcgcccgc gccggccgat accggccaat ctcggcgcct atgccgtgcc gctcgcgccc
     7081 ggcggaaacg tccgcgacgg cgttcatgcc atccggcgca agatcgaggc cttcgacagc
     7141 gcgccgttcc tgctttccat gcagcaattg ctggtccgga tcaccggcca tctgaacgaa
     7201 caccccaaac ccttcgtgct gttgctttcc tcggttcagc aggggggtga gcgcaacctt
     7261 gccgccgcgc tgatcgggct tggcttgcag cacatcaagg aacgggtgct ggttatcgaa
     7321 atcgccggcc agccatatcc ttacggcatg gttcagcgca gccggaagcc ggtggtcgac
     7381 ccggccagcg gattgccgac cgtggtggtc gatgccctgg tcatgacccc caacggtcca
     7441 gccgacaatg ccggcgcggt gatgcagatt ctggccgagc agcaaggcgc tttcggcttt
     7501 gcgctggtga tcgcccgccc gctggacgat cccgcctaca gctttgcctt ggcggcgcgt
     7561 gccgacctgg cgatctatgc gctgacgccc gcgcaacaac gcgccggcgc catctcgtgg
     7621 ataggccagc ggctcgcccc caagggggcc gccgatgggc ttgccaggac agccacggtc
     7681 gtcatcgaac aaaggatgga gcgcatgcag ccacgcgccg ccacagccga tggccggcgc
     7741 aagaccgccg cgcggggata gcgacgtgac gcggcaacag tcgccggcgg cgcgttcgac
     7801 gctgacccgt cggcaggcag tcggtggcgt ggcggccctg ctggccgcgc cgttgctgcc
     7861 gtcgcctggc atggccgatg ctgcgcctgt ggtcccgacg cgcggcttca accttcccgg
     7921 ctgggtcgac cgcgaggatg gcacagcgcc agcgaaggcg acgctcgaaa aattgcgggg
     7981 actgggtttt gaaacgatcc gcctgccggt cgccgccgat ccgctgctgt cgggcgcatc
     8041 cgcgcaagcg accatgctgg gcaggatcga tgcggcgata ggcgaggcgt tggccggcgg
     8101 cttcgccgtg atcgtcgatc tgcatcccgg cgaaaagttc gccgccgcgc tgcgcaacga
     8161 tccggcgcaa gggggcaaac aagccgtcgc ggcatgggat gcactggccg gcgtcgtcgc
     8221 gggccagcct gccggcaagg tgtatgctga actgttgaac gaaccgccga tgcagccggc
     8281 ggcatggctt gaactgcgcg accggctggc cgaaacggtg cgtgccaaat gccctcgcca
     8341 caccatcatc tggggcacgg cgccggtgca gggcatgtgg gaactggctg atgcgccgct
     8401 tctcgccgac gacaacgcca tcgtggcggt ccattactac acgccgatgg gcttcaccca
     8461 tcaatgcgag aactgggatg gctcgccgct tggccgcctc gccaacctgc cctttccggc
     8521 gacgaaggat gggccgcagg ccgcaagcct gttcaggaaa ttcaaggaaa ccggcgacac
     8581 ggacgccacc gccttgctga accaggaatt caccggcccg tggacggtcg agcacatcgc
     8641 gtccgacttt gcccggcttg gcgaatggtc gcgcaacaac cgctgcccgg ccatcgtcga
     8701 tgaattcggc gtgcttgatt tctgcgtcga tgcgccaagc cgcggcaact gggtgcgcgc
     8761 ggtgcggcag gcggcggagg ccaacggcat aggctgggcc tattgggaac tggatcaggg
     8821 tttcggcttc atcaaggatc gtaccagcac cgaaggcttc gacagcgcga tgatcgatgc
     8881 cttggtgaag ggatagcggc gatgtcgagt gccgtctctc atccaggcaa ggcccccgcc
     8941 cgccgcatgc cgcaatcggg cgcggaggcc gcgcttcaca ttgtcgtggc tctgctgctg
     9001 attggcgccg ccgtatcggg ctttgccgtt tcgacggtgt ttggcgcggt ggccagcctg
     9061 gtgctgacgc tttgcgtggc gacagcgctg cccgccgcca tgccggtggt gatcgtctgc
     9121 gccttcctgt tccagaacat gatcgtcgcc tggttcacgc cctacattcc cgacaacaac
     9181 gcgttcgacg cgctgcgcgg cgctaatttc gtggtgttga tgaccgccta tgccgcattc
     9241 ctggccgcgt ccttgcaggt gcggttgcgg gcggtgacac aggcccggcc ctggctttta
     9301 tggggcatcg cgctggccgg catcatcacc ttctacatgc tgcttggcgc ggtgcgtggc
     9361 gggccgaagg attccatcat ctatttccgc aacacgatca cgccgctcgc ctgcttccat
     9421 atcggcatcg ttgccgccag cctctaccgc atcgacctgc gcaaaagcct gttgtggctg
     9481 ggcgccagcg ccatcgtcta tggctattgc gagctgatct tcaccatgga tttcctcggc
     9541 ctgttcaatg gtgatctcta catccagcgc gcgatccgcc gccagatcga aaccggtgtc
     9601 tgggaaaaag cgttgcagca gaccggcttc gtgctgcgcg ggttgcagga cgtgatgacg
     9661 accaccttct tcaacacgcc tttgttcgcc gacctgttcc cgccggtgtt ccgcatcggc
     9721 ggccctaatt tccacccgat ctcctatgcc tatgcgctgg gcatcctttc ggtctggctg
     9781 ctgttcaagg ggcgctggct gttgccgctg gcggccttgc cgctgctgtt ggtcatcggc
     9841 tcaaagggcg cgatgttcat ggtggtggtg gcgctggccg tgcggcttct gcaaggcccg
     9901 ctcggcatgc ggctgaccct gcttggcgtg ctcgccgtcg cggcggcatg gatcatcgcc
     9961 gccatcggct acggcgcgac ccatggcgac tatcatgtgc tgggcctgat cgccggcctg
    10021 cgcggttttc tcggcaatcc gctggggcag gggcttggca tcggtggcaa tctgtcgagc
    10081 accagcttca acctcgactg ggaccgcgcg caggcgcaag gcgtcgcttc ggttccggtt
    10141 gaaagcgcgg tcggcgtcat gctctaccag atgggcgtcg gcagcatcgt gttcttcggc
    10201 ttcctcgctg cccttgtcgc cagcgctagg cggcttcttt tgaaaaccgg caatgctgaa
    10261 ttcctgttcg ccttcgttgc catcgtcacc atttccacca acgccgtcct acaggaagaa
    10321 gcgttctatt cgccgctggc gctcggcttt tgcctgctgc tggtcggcac ggcgttcgga
    10381 acgtattggc gcgaggaaag cctggttgcg cggtcggccg cagcttcccg caccataccg
    10441 caagggcatg cagctttgcg cgccggatcg gcgaagggcg ttcggccata ggccgatcga
    10501 tcgacggcta ttgtcggtcg tgtgagcgcc cgaaccctgg cgaagggccg gcaggacagc
    10561 cttaatccgc ctgttcgatc acatcccggc gctgttgcca cgtaccggcg gtctgcggct
    10621 tgacgaacag gctggtgacc ttgggcacgt cggctttcag tctggcctcc agccgttcga
    10681 cgcaggcttc gatctccggc gcagcgagat ggtcctcgaa ttcgacgctg acgccggcca
    10741 cgatctgatc cggtccgaga tgaacggtga gaacgccatt ggctcgttgc acagccgggt
    10801 cttgctgaac gatttccagt attttctgtt gcgtggccgg tgaagcgggt tcgcccatca
    10861 gcaggctctt gctctcgcgc gccaggaaga tcgcggttga accgaggatc accgcgatgc
    10921 cgagcgaggc aaccccatcc agttccggca tgttcagcat ttgagcggcg aatatgccgg
    10981 cgaaggcgac ggcgaggccg agcagggcag cgctgtcttc gaacaggatg gtatagacgc
    11041 tggggtcctt gcttacccgc acggccttga accagccaag cctgcccttg gcctggcgga
    11101 attccttgag cgcgatccac cacgaactgc cttcgaacag gacggaaagg ccgagcacga
    11161 tataattcac cttggcgttg acgacaggtt ccggcgcaat gatgtggacg atgccttcgt
    11221 agaatgaaac gcccgcgccg agcgcgaaga caaggagggc gacgatgaag ctccagaaat
    11281 aaagctcgcg cccatggcca agcggatggg tgcggtcggc gggccgcgag gcgcggtgca
    11341 aaccgtaaag caaaaggccg ccatttcccg tatcgaccag cgaatggaca ccctccgaaa
    11401 gcatggcgga actgccggtg aaaaaggcgg cggcgaactt ggtgatcgcg atcgccatat
    11461 tgccagcaag ggcggcatag atgaccgtcc tggaactgtt gtgcgccgcc atcacatccc
    11521 cttcatgagt tagcccccgc aacatagcgt ctcaaccgcc gaagcccagc cctcgttgcc
    11581 ggaagggcct tgcgaatgcc ccaaactctt ggttcactca gcttgaaatc aaacgcagga
    11641 gcgaatcatg gccaagggtg cgatgaaggc gggcaaggaa gcccgaaagc cgaagaagga
    11701 tgccaagaaa ctcgtgccgg cggcaccggc tctgaaggca ccgccggtga aggcgacacg
    11761 gctgaaggaa aaataaccgt ggccggccgt tatcggccgg cggatcgggc tgggccggtc
    11821 tttctccggg catttgcctg aagtacgaac gaatattggc ggggccggca tcgttgcccg
    11881 ccagatgcag cattgcccgc atggacgacc ttgcggttca aaaaaacgag ccgggacggc
    11941 ccgctgatcg agggagcgcc ccatggcgtg gacaatattg ttttttgccg gcttgtttga
    12001 ggtgggttgg gcgataggcc tgaaatacac ggaaggcttt tcccggccac tgcccaccat
    12061 tttgacggtt gtgtcgatga tcgtcagcct ggccctgctc gggctggcgc tgaagacgct
    12121 tccggtcggt accgcctatg cggtctggac ggggatcggc acggtcggca ccgcgctcct
    12181 cggcgtctgg ttgctgggcg agcctgccac cgcaataagg cttgcctgca tcgcgctgat
    12241 cgtctgcggc atagccgggc tgaaactcgc cgcgtagagc gcgtcgtcgg gccggaagca
    12301 ttccgaacgt aaaaatgaaa cgaccggaat tacctgaggc aattccggtc gtttttattt
    12361 gatggctgca ccgggccaga atcaacggat agcgtcgccg gtcgctgaat caatggatgc
    12421 ttgccgtcgc gctgatcaac ggtccagccg cgatgcgttc tgctttgtgc atcgaccggg
    12481 aaagcagcga cagcctgtcc cggaatgcct taacgcgcgg agaaacccgg aggcgtgcgg
    12541 ccggtgcgct gcttgcgtgc cgcatagcgc gcatcacgct ttgccttgcg ctcggcctcg
    12601 tcggccagca ggcgggcgat gcgctcggtt tcctcggcgt cgcgaagttt tgcttccgcc
    12661 tgggctgcct cttcggcggc gatccgcgcc gcttcggcgg cggcttcgcg ctcggccttg
    12721 tcagccgcct cgcgggccag tctttcctgc ttcagcgttg ccttctcggc ttcgcgcttt
    12781 tcgcgcgcct cgaggatggc cttgcgttcg gcctgccgcg ccagcactgc tggatcatct
    12841 gccgccggct ttgccttgaa gcgctccagc agcgccttct ttgcctcgtt tgcggcattg
    12901 cgccgctcga aaacgtcttt ttccctatag atagccaagt ccacttccct gaagtcaatt
    12961 cgcgaggctt acatacgcgc gaagcccgag aattcaagcg ccaaatcggc ataactgata
    13021 tgaaattcca gcctgttgcc gcaaaaagac actggtatcg gtgcaggttg cccaatatgc
    13081 ggcgcccttt ggcgtcagcg cggtggcaaa cggcgacaag ccgcccggcg tcttcctccg
    13141 ctgcgcggca tcaatcgtcg ccttcgacaa ccctgaggcg aagctgtccc gtctttgaat
    13201 cggcaacgcg cctggcctgg ccctcgccgt ccgccgtccc ggcttgcccg cccgcatcct
    13261 gtcccggcgc gcgaagttcc agcgcctcga tcttctggcc gcgcttggta actttcgacg
    13321 tcgataccag aatgtcttct atatccttgg ccgactggcc gaaatgcgtc tgcaacttgc
    13381 gcacgcgctc gtcgacgcgc gccacgtcct ccatcagccg cacgacctcg ccctggatga
    13441 gatgggcctg ttcgcgcatg cgcgcatcct tgaggatggc ctggatgacc tggatcgaca
    13501 gcatcagcag cgacggcgag acgatgacga tgcgcgcccg gtgcgccttg tggacggtac
    13561 cctcgaaatt ctcatggatt tcggcaaaca ccgattccga aggcacgaac atgaaggccg
    13621 tgtcctgcgt ttcgccctgg atcaggtatt tctccgaaat cgcgcgcaca tgcacctcga
    13681 tgtcgcggcg aaacgcctgt gcggcgacct tttgcaattc ggggctttcg gccgcccgta
    13741 tggcgttcca ggcttccagc ggaaacttgg cgtcgatcgc cagcgaaggc gcgccattcg
    13801 gcatcttcac aaggcagtcg ggccggctgc cgttcgacag tgttgcctgg aattcatagg
    13861 aacccatcgg caggccgtcg gcgacgatgg cttccatgcg cgattgcccg aacgcgccgc
    13921 gcgtctgctt gttggaaagg atcgcctgca attgcaccac ctgcccggcg agcgactgga
    13981 tattgccttg cgcggtatcg atgatggcca gccgctcctg tagcttggac aggctttcat
    14041 gggtggattt cgtctgctcg gtcatcgtct ggcccagccg gccggtcatg gcgtcgagcc
    14101 gctggccgat ggattgggtg agttcggcct gccgcgcgcc gaacacctcg gcgatcgcgc
    14161 ccatgcggcc ctgcatctcg gcctggcttt gtaaaatgcc ggccatgcgc gcctccgcct
    14221 cgcgggcgtg gtcggctgcc tcggccgcgg ccatcgcgcg ggcgtgcgac gagcgccaca
    14281 gcgcaacggc cagccaggcg agcgccagca acaaaaggat cgcgccgaac gacagcgcct
    14341 ggcccagcgt gattgtcgtc gcgccaagcc ggatgatcgg ctccgaaatg gtaatgcctg
    14401 tctcattcat gcgactacct tagccgattc ggcccgcttc accagatcaa aacgtgaaca
    14461 atccccggtg gtggcggttg acgggttttg tctgaggtct taggtgaggc gaatgtcgat
    14521 caagccgctc atcattcttc ccgacccgct tctgcgccag ctgtcgaaac cgcttgagcg
    14581 ggtcgatgcg caggtggcca ggctggccga cgacatgctg gaaaccatgt atgatgcgcc
    14641 cggcatcggg ctggccgcaa tccagatagg cgagccgctg cgcatgttgg tcgtcgacct
    14701 cgccaaggag gacgaggcgc cggcgccgca tgtcttcatc aatcccgaaa tcctccagcg
    14761 ctcggacgag cgctcggtct acgaggaggg ttgcctgtcg atcccggatt attacgccga
    14821 ggtcgagcgt ccggcatcgg tgcgcgtgaa atatctcgac cgcgacggca acagccagga
    14881 gatcgaggcc gagggcctga tggcgacctg cctgcaacat gagatcgacc atctcgacgg
    14941 cgtcctgttc gtcgaccaca tctccaagct gaagcgcgac atcgtcgtgc ggaagttcaa
    15001 gaagcttgcc aaggacaggg cgccgggcaa gctggtcgga taggagcgca aatgccgctt
    15061 cgcctcgttt tcatgggtac gccggatttt tccgtgccga cgctgcgcgc gctggcaggc
    15121 gccggccacg atatagccgc cgtctatacg cagccgccgc gcgccggcgg ccggcgcggg
    15181 ctggaattga cgccgtcgcc cgtgcagggc gaggcggagc gcctgggcgt cgaggtccgc
    15241 gcgccggcgt cgttgaaagg cgaggccgaa cagcaggcgt tccgcgccct gggggcggat
    15301 gtcgcggtcg tcattgccta tgggctgctg ttgccgcaag ccattctcga tgcgccgcgc
    15361 cttgcctgcc tcaacggcca cgcctcgctt ttgccgcgct ggcgcggggc cgcccccatc
    15421 cagcgcgcca tcatggcggg cgacgccgag accggcatga tggtgatgaa gatggaagcg
    15481 gggctggaca ccggcccggt tgccttggtg gacaaggtgg cgatagccgc cgacatgacc
    15541 gccggcgaac tgcacgaccg gttgatggat gccggcgcga ggctgatggt cgaggcggtg
    15601 gcggcgctgg aagcgggccg gctcgccttc tcgccgcagg cggctgaagg ggtgacctac
    15661 gcccaaaaaa tcgataagtc ggaaacccgc gtcgactgga ccctttcggc aaccgaggtg
    15721 cataaccata ttcgcggcct gtcgccgttt cccggcgcct ggtgcgaagt ggaaatcggc
    15781 ggacgccggg agcggctgaa attgctgcgc tctgccatgg ccgagggcct tggcgaaccg
    15841 ggaggcattt tggacgaccg gctgacggtg gcctgcgggg caggcgcggt caggctggtc
    15901 gaagtgcaac gggcgggcgg caagcccgtc gccgcgtcgg agtttctgcg cggcacacgg
    15961 ttggaaaaag gaatgaaatt atcatgagca tgatgtcgat acgggcggcc acgccgcgcg
    16021 accgcgacgc aatccggctc gtcgaagaac atgcgttcgg ccagcaggcg gaagccgggc
    16081 tggtcgacgc cttgatcgag ggcggcgacg ccatcgtcga actggtggcg gtggaagacg
    16141 ggcaggtcgt cggccatatt ctgttttcgc gcctttacgt gcagcaaggc ggccggaaat
    16201 atccagccgt ggcgctggcg ccgctggcgg tggaacattc gttccatggc accggcattg
    16261 gcggcgcgct catccgcgag gcgcatatcc ggctgaaggc cgccggcgag acactttcta
    16321 tcgtgcttgg cgacccggcc tattacggcc gcttcggcta cagccacgaa cgggccgaaa
    16381 aattcgaaag cgcctatcag ggcgaggcgc tgcaagcttt gtcgtggggc gaggcgccgc
    16441 aagccggcct gctcgtctat gcttcggcct tcgaagcgct cgccgcctga ccgcgttgcc
    16501 ggggcatggc gcgttttcgt ctcgatatag aatatgacgg cggcgcctat gccggctggc
    16561 aacgccaggc cgaccagcct tccgtgcagc aggcgataga acaggcgatc gccaggttct
    16621 cgggcgacgc cgtctcgctg cgcggcgccg gccgcaccga tgccggcgtc catgcatcgg
    16681 gtcaggtcgc ccatgctgat ctgacgaagg attggacggg cgacaaggtg cgcgatgccg
    16741 tcaacgcgca tctgcaaatg gcgggcgagc gggtgtcgat cctcaaggcg acacccgtgg
    16801 cggatgattt cgacgcgcgc ttttcagcac ttggccggca ctatctctac cgcatcgtca
    16861 accggcgctc gccgccggcg ctcgaaatgg gcaaggtctg gtgggtgccg aagccgctcg
    16921 acgcgcaggc catgcacgag gccgccaaag tgctgctcgg ccggcacgat ttcaccacct
    16981 tccgctccac ccagtgccag gccaacagcc cggtgcggac gctggcccgc ctcgatgtca
    17041 cccgcgaggg cgacgtaatc gagatacgcg cctcggcccg ctcgttcctg cacaaccagg
    17101 tgcgctcgat caccggttcg ctgaagcgtg tcggcgacgg cggctggacg gcggacgacc
    17161 tgaaggccgc gctggaggcg cgcgaccgcg ccgcctgcgg gcaggtcgcg ccgccggacg
    17221 ggctttatct gaccaaggtg gattatcccg aagcggaatg atccggcccg cttggagcat
    17281 ttccagcaaa gtgtgaaacg gttttgcgtc cgaaaatgcg gccagagata gagcggtttt
    17341 accgattcgg taaaaaccag aacggctcta gcccggaaaa ctgatgccga gcagcgcctg
    17401 tagcgcaaac aataccagca gcgagacgac gaacatgccg gcgaaaacgg cggtgccgac
    17461 tgccgcgccc ttgccgatcg ccgcgttggt catgcgccag gtgagtacca tcgacagcag
    17521 gaagcagaag aacgagaaaa agttcgctat tgaatccgga tcgggcatga cgaggcggat
    17581 aagggccggc ggcagcatca gccaggcgac gatggcggaa gcccagttgc tggccaccac
    17641 ataggcgacg aagcgcccgc caatgccggc atggggcgcg acgaaggcga ggccgaccag
    17701 cggcagtacc catgcgccga gatcgaccgc cgccagccga agcagcgtgt cgaagcgccc
    17761 ggcgaaagcg gccgggtcgg cgacctcgtt gacgacgctg acccagccga caatgagcgc
    17821 cggcaaagcg acgacaatgg caaagaagga attccagaac ccgtcggcgg aaaggtcgag
    17881 cagacgaagc ccgtcggcct tgcccagcat caacttccat gcaccggcga gcgaagcctg
    17941 tgtttcgtcc gccgacagca gcatcgaacc gttccctcag cccttctcga accagtcttc
    18001 gatgaaccgc ccgtagattt gggtcagctt ttcgagatcg gcaagggcaa ccctttcatc
    18061 gaccatatgc atggtctggc cgaccaggcc gaactcgacg accgggcaat aatccttgat
    18121 gaagcgcgca tccgaggtgc cgccggaagt ggaaagcgcc ggttccttgc cgatcaccgc
    18181 gccgattgag ccgctcagcg tctcgatcag cctgtcgtcg cgggtcagga agacgtggct
    18241 tggccggtcg cgccagacca gttcgtaatc gaccggctcc ttgcggccgg cgcggtattt
    18301 cctgcggcgc gctgcctggt cgaggcggtt gtggatttcc gcctgcatcg tctcggcgct
    18361 ccatgtgtcg ttgaagcgga tgttgaaggt ggccgtcgcc ttggccggaa tgacattggt
    18421 ggccggattg ccgacgtcga tggaggtaac ctccagattg gtcggctgga aatccttcgt
    18481 gcccttgtcg aaggccggcg caagcagcgc gtcgaccagg ctcatcaggc cgcgtaccgg
    18541 gttgtcggcc agttgcggat aggcgacatg gccctggcgg ccattgacgg tgacggtgcc
    18601 cgacagcgat ccgcgccggc cgatcttgat ggcgtcgcca agcgcatccg ggttggtcgg
    18661 ctcgccgacg atggctgcat cccacgtctc gcccttggcg gccgcccagt cgagcagctt
    18721 ggtcgtgccg ttgatggccg ggccttcctc gtcgccggta atcagcagcg agatcgagcc
    18781 tttcggtccg ccgtgctttt cgacatggcg ggcaacggct gcgatgaaac aggcgatgcc
    18841 gcccttcatg tcgacggcgc cgcggccata catttcacca ttggcgattg cggcggaaaa
    18901 gggtggatgc gtccacgccg cctcgtcgcc tgtcggcacc acgtcggtgt ggccggcgaa
    18961 catcaaatgc ggtccgttgc cggagcggcg cgcgtagagg ttttcgatgg ttggcgtgcc
    19021 gtcatccgaa aagctcggac gctcgacggc gaagccgagc ggctccagca tctgctgcaa
    19081 agccgccagc gcgccaccct cggccggcgt gacggaaggg cagcggatca ggatggcgag
    19141 gttctcggcg gggtcggtgg gcagggtcat ggcgtcagcg ataatcgaaa ggccgcttcc
    19201 tgtcaccagc gtagctttgg accaattgcg ccatcccgcc gtcatttcgg gcatcggttg
    19261 aaaacgctcc cgtccggcat cctaccggac gatgcgcaca tgcgcccggt agcgtccctt
    19321 gccggtgcgc ttggcttcgt acagcgcgtt gtcggaacag gacagcagaa cttccgggtc
    19381 gctgccgtcg cttggcgcaa aggcgctgcc gatgctcagg ccgatccgga ccgaagtggc
    19441 gacgccgagg tcgaacggtt ccgagatatc gtcgatggag cgctttgcca gataggtcgc
    19501 ctcgatttcg gaggtgttgg gcagcaggat gacgaa
//
LOCUS       DASYCS010000197         2034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3525686,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000197 DASYCS010000000
VERSION     DASYCS010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3525686"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="VGM46_06395"
     CDS             <1..486
                     /locus_tag="VGM46_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEY4192258.1"
                     /translation="VLIEDGKLVGYMQDRQNARLMGMKATGNGRREGYAHQPMPRMTN
                     TYMTGGDMEPEEIIASVKNGIYAVSFGGGQVDITSGKFVFGCTEAYMIENGKVTQPIK
                     GAMLIGNGPDAMHRVSMIGNDMKLDTGIGMCGKAGQGVPVGVGQPHLRMNQMTVGGTR
                     V"
     gene            complement(635..1876)
                     /locus_tag="VGM46_06400"
     CDS             complement(635..1876)
                     /locus_tag="VGM46_06400"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEY4192259.1"
                     /translation="MTARFEIVKLGSQGDGIAETGQGDVFIPFALPGETVNAAREKDR
                     ATLMAVIDPSPLRIAPACRHFTECGGCAVQHLEAEAYREWKRAKVGHALKMKGIEAEI
                     GALVPCEPHSRRRVTFTARRTDGGMLFGFVRALSSEVISIVECPISLPEIMVALDGLK
                     ALAALICATTKSFHMTVTATTSGLDIAAQDSGKLGEHQRRIAANFVIAQRFARLSIDG
                     EIVVEPQKPVVLFDAVAVNVPSGAFLQATQPAEQAMAALVGQHLARGKKVADLFAGCG
                     SFALRLASNSEVHAVEGDAAALAALDHGFRFAAGLKRVTHERRDLFRRPLTFKELNGF
                     DGLVFDPPRAGAEDQSKQIARSNVPLVAAVSCNPVTLARDLRILLDGGYRLKSVTPID
                     QFLWSPHVETVALLEKPKQRR"
     gene            complement(1873..>2034)
                     /locus_tag="VGM46_06405"
     CDS             complement(1873..>2034)
                     /locus_tag="VGM46_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008125253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family rRNA
                     (cytidine-2'-O)-methyltransferase"
                     /protein_id="HEY4192260.1"
                     /translation="GLLKNPDDAGRMAQELRQWLDAVPGWRALGVHPSPIEGGDGNRE
                     FLLAGEKTE"
BASE COUNT          386 a          642 c          668 g          338 t
ORIGIN      
        1 gtgctgatcg aggacggcaa gcttgtcggc tacatgcagg accggcaaaa tgcccggctg
       61 atgggcatga aggcgaccgg caacggacgg cgcgagggct acgcgcacca gccgatgccg
      121 cgcatgacca acacctatat gaccggcggc gacatggagc cggaagagat catcgcttcg
      181 gtgaagaacg gcatctatgc cgtttcgttc ggaggcggcc aggtcgacat cacatcgggc
      241 aagttcgtgt ttggctgcac ggaggcctac atgatcgaga acggcaaggt gacgcagccg
      301 atcaagggcg cgatgctgat cggcaacggg ccggacgcca tgcaccgcgt ttccatgatc
      361 ggcaacgaca tgaagctcga caccggtatc ggcatgtgcg gcaaggccgg gcaaggcgtg
      421 ccggtcggcg tcggccaacc gcatctgcgc atgaaccaga tgacggtggg cggcacgcga
      481 gtctagaggc gagatagccg gcgcgccgaa aggaacgagg tcttttttcg taggaccgca
      541 gggcggacat atccaaggat accgcgcgca tgccgttctc tttgcatgct tgaacggtca
      601 atgctcaaac ggcgcgccat tcaggcaccg gcgttcaccg ccgctgcttc ggcttttcaa
      661 gcagcgcaac cgtttcgaca tgcggcgacc ataggaactg gtcgatgggc gtcacgctct
      721 tcaggcggta accgccgtcg agcaggatgc gcaggtcgcg cgccagcgtg accgggttgc
      781 acgagacggc ggcgacgagc ggcacgtttg agcgcgcgat ctgcttcgac tgatcctcgg
      841 cgccggcgcg cggcggatcg aacaccagcc cgtcgaagcc gttcaactcc ttgaaggtga
      901 gcgggcgccg gaacaggtcc cgccgctcat gggtgacacg cttgaggccg gcagcgaaac
      961 gaaagccgtg atccagcgcg gcaagggcgg ccgcatcgcc ttccactgca tggacttccg
     1021 aattcgaggc aagccgcaac gcgaagctgc cgcagccggc gaaaagatcg gccaccttct
     1081 tgccccgcgc cagatgttgg ccgaccagcg cggccatcgc ctgctccgcc ggctgggtgg
     1141 cttgcaggaa agcgcccgac ggcacgttta ccgccaccgc atcgaacaag accaccggct
     1201 tttgcggctc gacgacgatt tcgccgtcga tcgacaggcg ggcaaatctt tgcgcgatga
     1261 cgaaattggc ggcgatacgg cgctggtgtt cgcccagttt gccggaatcc tgcgcggcta
     1321 tgtcgaggcc tgaagtggtc gccgtcaccg tcatgtggaa agacttggtc gtcgcgcaga
     1381 tgagtgcggc aagggccttc aacccatcaa gcgcgaccat gatttccggc agcgaaatcg
     1441 ggcattcgac gatcgaaatg acttcgctgg aaagggcgcg gacaaaaccg aacagcatgc
     1501 cgccgtccgt ccggcgcgcg gtaaacgtca cccggcggcg gctatgcggc tcgcagggca
     1561 ccagcgcgcc gatctcggcc tcgatgccct tcatcttcag cgcgtggccg accttggcgc
     1621 gcttccactc ccgataggct tccgcctcca gatgctgcac ggcgcagccg ccgcattcgg
     1681 tgaaatgacg gcaggccggc gcgatccgga gcggcgaggg atcgatcacc gccatcagcg
     1741 tggcacggtc cttctcacgc gccgcattga cggtttcgcc gggcagcgcg aaggggataa
     1801 agacatcgcc ctgcccggtt tcggcgatgc cgtcgccctg tgagccgagc ttcacgattt
     1861 cgaaccgtgc ggtcattcgg ttttttctcc ggcaagcagg aattcgcggt tgccgtcgcc
     1921 gccttcaatg ggtgatggat gaacaccgag cgcgcgccag ccgggcacgg cgtcgagcca
     1981 ttgccgcaat tcctgcgcca tgcgcccggc atcgtcggga tttttcagca ggcc
//
LOCUS       DASYCS010000198         5201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4540237,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000198 DASYCS010000000
VERSION     DASYCS010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 5201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5201
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4540237"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1744
                     /locus_tag="VGM46_06410"
     CDS             <1..1744
                     /locus_tag="VGM46_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006336061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEY4192261.1"
                     /translation="EIYEQIKRHKTTLLFVNTRSQAELLFRELWQVNEDTLPIALHHG
                     SLDVGQRRRVEKAMGDNALRAIVATSTLDLGIDWGDVDLVVHVGAPKGASRLAQRIGR
                     ANHRMDEPSKAILIPANRFEVLECRAALDANYLGAQDTPPLTDGGLDVLAQHVLGCAC
                     GAPFLADDLFREIRTAAPYAKLERTVFDRVVDFVATGGYALKSYERYARIRKNKDGLW
                     RVSSPRVAQQYRLNVGTIIEVPALNVRYVKAGAKASSARGGMVLGKIEEAFLETLTHG
                     DTFLFAGKILRFEGIRENECFVSSAPGKDAKVPYYGGGKFPLSTYLASQVRAMLDDPA
                     RWKLLPEQVADWLRIQAEKSVLPKRSDLLIETFPRSNRHYLVAYPFEGRLAHQTLGML
                     LTRRLERAGARPLGFVATDYALAIWSLGDMGAMFKAGKPSLAALFDEDMLGDDLEAWL
                     ADSWLLKRTFRACALISGLIEKRHPGQEKSGRQVTVSTDLIYDVLRSHEPDHILLQAT
                     RSDAAAGLLDVRRLADMLSRISGRIVHKQLGHISPLAVPIMLEVGKMPVNGEADETLL
                     MDAADLVEEAMGQR"
     gene            1762..2454
                     /gene="pdeM"
                     /locus_tag="VGM46_06415"
     CDS             1762..2454
                     /gene="pdeM"
                     /locus_tag="VGM46_06415"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008594862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     endonuclease PdeM"
                     /protein_id="HEY4192262.1"
                     /translation="MADTRRDLIEVAGERALCDRRGVLYFPDHGLLAVSDLHLEKGSS
                     LARRGALLPPYDTAATLVRLQAVIGDYQPAVVVSLGDSFHDGAGAARMHSSFREHLES
                     LMAGRDWFWVAGNHDPEAPAGLSGTTVREILVGALSFRHEPSRQRIEGEISGHLHPCA
                     RIVQRGRSVRRRCFAGDGGRMIMPAFGAYTGSLNVLDRAYAGLFRRESLTAYMLGAKR
                     IFALPGSMLQPG"
     gene            complement(2513..3304)
                     /locus_tag="VGM46_06420"
     CDS             complement(2513..3304)
                     /locus_tag="VGM46_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015319314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02186 family protein"
                     /protein_id="HEY4192263.1"
                     /translation="MPGLKPLNIAALLAALLAPQAAHAQLVHNEGIQIGLSTDNVAIT
                     AGFSGADLTIFGSLEDADPQIARQGRYDVIVVLEGPGQPVVVRRKDRVLGVWVNTQSE
                     TFENVPVSYSLASTRPLQDITEPNSYKQLSLGLGNIYMQPAETDSNATDIQDFTKALR
                     QRKSASGLYSENVGGVQFLSQNLFRATVRLAPDVQVGTHRARAFLFKNGLFVKENSAQ
                     LEIRKSGFEQMVFRLAHNHSIFYGVFAISLAMLTGWLGRVIFRKD"
     gene            complement(3304..4227)
                     /locus_tag="VGM46_06425"
     CDS             complement(3304..4227)
                     /locus_tag="VGM46_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY4192264.1"
                     /translation="MGIYLPIAEISVNVFVLLAMGTAVGFLSGMFGVGGGFLITPLLI
                     FYNIPPAIAVATGANQVIASSFSGALSHMKRGTLDFKLGGVLLAGGVFGSTGGIFVFG
                     LLRRLGQLDLFISLLYVVLLGTVGGLMLVESVNALRMTRSGAAPVLKKSGQHNWIHRL
                     PLKMRFRASKLFVSVIPVLALGAAIGFLSSIMGVGGGFIMVPALIYLLKVPTNVVIGT
                     SLFQIIFTSAYTTLIHSATNQTVDVILAFVLMAGGVAGAQYGAKVGQKLRGEQLRALL
                     ALLVLAVAIRLAIGLFVVPPDLYSLSALGLD"
     gene            complement(4408..>5201)
                     /locus_tag="VGM46_06430"
     CDS             complement(4408..>5201)
                     /locus_tag="VGM46_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015319312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HEY4192265.1"
                     /translation="KALFEVASRYAEARGVKQDMAAAAKWYEKSAELGLAPAEYRIGN
                     FYEKGIGVTRDIQKAKTWYQLAAAQGNASAMHNLAVLFAMAADGVTDNESATRWFQSA
                     ADLGVKDSQFNLGILAAKGVGMKQNLEDAYKWFALVAKTGDKDAASKRDEIAHALRPE
                     QLEQARATTELWKAKPVDVAANSVDIPESWQEGTPSTTAGIDMKKAVQNIQRILNKNG
                     YDAGGTDGVMGQKTKNAIVAFQGDNGMPATGEVDEKLVKSLLAHK"
BASE COUNT          929 a         1599 c         1732 g          941 t
ORIGIN      
        1 cgagatttac gagcagatca agcggcacaa gaccacgctc ctgttcgtca acacgcgcag
       61 ccaggccgaa cttttgtttc gcgaattgtg gcaggtcaac gaggatacgc tgccgattgc
      121 gctgcatcac ggctcgctcg atgtcggcca gcgccgccgc gtcgagaagg cgatgggcga
      181 caatgcgctt cgggccatcg tcgccacctc gacgctcgat ctcggcatcg attggggcga
      241 tgtcgatctg gtcgtgcatg tcggcgcgcc gaagggggcg agccggctgg cgcagcgcat
      301 cgggcgcgcc aaccaccgca tggacgagcc gtcgaaggcg atcctcattc cggccaaccg
      361 cttcgaagtg ctggaatgcc gcgcagcact cgacgccaat tatctgggcg cgcaggatac
      421 gccgccgctg accgatggcg ggctggatgt gctggcgcag catgtgctgg gctgcgcctg
      481 cggcgcgccc ttcctggcgg acgatctgtt ccgtgaaatc cgcacggcgg cgccttacgc
      541 aaaacttgag cggacagttt tcgaccgcgt ggtcgatttc gtcgccaccg gcggctacgc
      601 gctgaaaagt tatgagcgct atgcccgcat tcgcaagaac aaggacgggc tgtggcgggt
      661 ttccagcccg cgcgtcgccc agcaataccg gctgaatgtc ggcaccatca tcgaagtgcc
      721 ggcgctcaac gtgcgctatg tgaaggccgg ggcgaaggct tcgtccgcgc gcggcggcat
      781 ggtgctgggc aagatcgaag aggcattcct cgaaacgctg acgcatggcg acactttcct
      841 gttcgccgga aaaatcctgc gcttcgaggg catccgcgaa aatgaatgct tcgtttccag
      901 cgcaccgggc aaggacgcca aggtgccgta ttatggcggc ggcaagttcc cgctctccac
      961 ctatctggca agccaggtcc gcgccatgct ggacgatccg gcgcgctgga agctgctgcc
     1021 ggagcaggtg gccgactggc tgcgcataca ggccgaaaaa tcggtgctgc ccaagcgcag
     1081 cgacctgttg atcgagacgt ttccgcgcag caaccggcat tatctggtgg cctatccgtt
     1141 cgagggaagg ctggcgcacc agacgctcgg catgctgttg acccgcaggc tggagcgggc
     1201 gggcgcgcgg ccgctcggtt tcgtcgccac cgattatgcg ctggcgatct ggtcgctggg
     1261 cgacatgggc gcgatgttca aggcggggaa gccgtcgctg gcggccctgt tcgacgagga
     1321 catgctgggc gacgacctgg aggcgtggct ggccgacagc tggcttttga agcgaacctt
     1381 ccgcgcctgc gcgctgattt cgggcctgat cgaaaagcgc catcccggcc aagaaaagag
     1441 cgggcggcag gtgacggttt cgaccgatct catctatgac gtgctgcgca gccacgagcc
     1501 ggaccatatt ctgttgcagg cgacgcggtc ggacgcggcc gccgggctgc tcgatgtcag
     1561 gagactggcc gacatgctct cgcgcataag cgggcgaatc gtgcataagc agcttgggca
     1621 tatctcgccg ctggcggtgc cgatcatgct ggaagtcggc aagatgccgg tcaacggcga
     1681 agccgacgag acgcttctga tggatgccgc cgatctggtc gaggaggcca tgggccaacg
     1741 atgaactttg ccaccgccag catggccgac acaaggcgcg accttatcga ggtcgccggc
     1801 gagcgtgcgc tgtgcgaccg gcgcggggtg ctgtacttcc ccgatcacgg cttgctggcc
     1861 gtatccgacc tgcatctcga aaaaggttcg tcgctggcgc ggcgcggcgc gctgttgccg
     1921 ccctatgaca cggcggcgac gcttgtccgg ctccaggcgg ttatcggcga ctatcagccg
     1981 gccgtcgtcg tcagcctcgg cgacagcttt cacgacggtg cgggggcggc gcgcatgcat
     2041 tcgagctttc gcgagcatct ggaaagcctg atggccgggc gcgactggtt ctgggtcgcc
     2101 ggcaaccacg atcccgaagc gcctgccgga ctgtccggca cgaccgtgcg cgagattctg
     2161 gtcggcgcgc tttcgtttcg gcacgagccg tcgaggcagc ggatcgaggg cgaaatttca
     2221 ggccatctgc acccttgcgc gcgcatcgtg cagcgcgggc gctcggtgcg gcggcgttgt
     2281 ttcgccggcg atggcgggcg catgatcatg ccggccttcg gcgcctatac cggctcgctc
     2341 aacgtgctgg accgcgccta tgccgggctg tttcgccgtg aaagcctgac cgcctacatg
     2401 ctgggcgcca agcgcatctt cgcgcttccc ggctcgatgc tgcaacccgg ctgatcgacc
     2461 tggagcaccc gtgggaagca ctcttctgga gcacccttcg ggcggccttg catcaatcct
     2521 tgcggaagat gacgcgcccg agccagcccg tcagcattgc gaggctgatg gcgaagacgc
     2581 catagaagat ggaatgattg tgggcgaggc gaaacaccat ctgctcgaag cccgatttgc
     2641 ggatttcgag ctgcgctgaa ttttccttca cgaacaggcc gttcttgaac aggaacgcgc
     2701 gcgccctgtg ggtgccgacc tgcacgtcgg gggcaagccg cacggtggcg cggaaaagat
     2761 tttgcgacag gaactgcacg ccgccgacat tttcgctgta gaggccgctc gccgatttgc
     2821 gctggcgcag cgccttggtg aaatcctgga tgtcggtggc gttgctgtcg gtctcggccg
     2881 gctgcatgta gatgttgccg aggcctagcg acagctgctt gtagctgttc ggctccgtaa
     2941 tatcctgcaa cggcctggtg ctggccagcg aataggagac cggcacattt tcgaaagttt
     3001 ccgattgcgt gttgacccag acgccgagca cgcggtcctt gcgcctgacc accacaggct
     3061 gccccggccc ttccagcacc acgatgacat cgtagcggcc ctgcctggcg atctgtggat
     3121 cggcgtcttc gagcgagccg aagatggtga ggtcggcgcc ggaaaaaccc gcggtgatgg
     3181 cgacattgtc ggtggaaagc ccgatctgga tgccttcatt gtgtaccagc tgcgcatgcg
     3241 cggcttgcgg cgcgagcagg gcggccagca gggcggcgat gttcaggggc ttcaatccgg
     3301 gcatcaatcc agccccagcg cggaaagcga ataaagatcg ggcggcacca cgaacaggcc
     3361 gatggcaaga cggatggcaa ccgccagcac cagcaatgcc agcaaggcgc gcagctgttc
     3421 gccgcgcagt ttctggccga ccttggcgcc atattgcgcg ccggcgacgc cgcctgccat
     3481 cagcacgaac gccaggatga cgtcgacggt ctggttggtc gccgaatgga tgagcgtcgt
     3541 ataggccgat gtgaagatga tctggaacaa cgaggtgccg atgacgacgt tggtcggaac
     3601 cttcagcagg tagatcagcg ccggcaccat gatgaagccg ccgccgacac ccatgatcga
     3661 tgacaggaag ccgatggccg cgccgagcgc cagcaccgga atgacgctga cgaacagctt
     3721 ggaggcccta aagcgcatct tcagcggcag gcggtggatc cagttgtgct ggccggattt
     3781 cttgagcacg ggcgccgcgc cgctgcgggt catgcgcagc gcattgacgc tttcgaccag
     3841 catcaggccg ccgacggtgc cgagcaggac gacataaagc agcgagatga acaggtcgag
     3901 ctggccaagc cggcgcaaga ggccgaacac gaatatgccg cccgtcgagc cgaacacgcc
     3961 gccggcgagc agcaccccgc caagcttgaa gtcgagcgtg ccgcgcttca tgtgcgacag
     4021 cgcgccggag aaggaagagg cgatgacctg gttggcgccg gtggcgacgg cgatggccgg
     4081 ggggatgttg tagaagatca gcagcggggt gatgaggaag ccgccgccga cgccgaacat
     4141 gcccgacagg aagccgaccg cagtgcccat ggccagcagc acgaagacgt tgacggaaat
     4201 ttccgcgatc gggagataga tgcccacccg tcagtctcga tcaatttgcc cgtcggccga
     4261 catggccgct tttgttcata gcaccgaaaa gctaacccgt tcttgccgca acggcgcaac
     4321 cgcgattgtg acgccgaaac aaaaactatc tcaatcggtt agcaggcaaa tttgtggcac
     4381 ggcgtcgtgg aagcgacgcc aatgcggcta tttgtgcgca agcagcgact tcaccagctt
     4441 ttcatccact tcgcccgtcg ccggcatgcc gttgtcgccc tggaaggcga cgatggcgtt
     4501 cttcgtcttc tggcccatca cgccatcggt cccaccggcg tcgtagccgt tcttgtttag
     4561 gatgcgctgg atgttctgca cggccttctt catgtcgatg ccggcggtcg tggagggcgt
     4621 gccctcctgc caggattcgg gaatgtcgac cgaattggcg gccacatcca cgggctttgc
     4681 cttccacaac tccgtcgtgg cgcgagcctg ctcaagctgc tcggggcgca gcgcatgggc
     4741 aatctcgtcg cgcttggaag ccgcatcctt gtcgcctgtc ttggcgacga gggcgaacca
     4801 tttataggcg tcttcgaggt tctgcttcat gccgacgccc ttggcggcca ggatgccgag
     4861 gttgaactgg ctgtccttga cgccgagatc ggcggcggac tggaaccagc gcgtggccga
     4921 ttcattgtcg gtgacgccat cggcggccat ggcgaagagc acggccagat tgtgcatggc
     4981 gctggcgttg ccctgggcgg cggcgagctg ataccaggtc ttggccttct ggatgtcgcg
     5041 ggtgacgccg atgccttttt cgtagaaatt gccgatgcgg tattccgccg gcgcaaggcc
     5101 gagttcggcg gatttttcat accatttggc ggcggccgcc atgtcctgct tgacgccgcg
     5161 cgcctctgca taacgcgacg cgacctcgaa cagcgccttg g
//
LOCUS       DASYCS010000199         8689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3091064,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000199 DASYCS010000000
VERSION     DASYCS010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 8689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8689
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3091064"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..325)
                     /locus_tag="VGM46_06435"
     CDS             complement(71..325)
                     /locus_tag="VGM46_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018326874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEY4192266.1"
                     /translation="MPVVNMLDAKTSLSRLVEAVESGAESEIIIARNGKPAARLVPVQ
                     TVKKTGRKLGMFEGEYPPFTLEEWNSTDAEIAEMFGVDKP"
     gene            complement(384..635)
                     /locus_tag="VGM46_06440"
     CDS             complement(384..635)
                     /locus_tag="VGM46_06440"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006330579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEY4192267.1"
                     /translation="MADEANEDVKAMSFEQALDALEKIVDDLERGDVPLDQSIRIYER
                     GEALKAHCDRLLKAAEDKVEKIRLSRDGKPAGTEPLDAE"
     gene            complement(638..1564)
                     /locus_tag="VGM46_06445"
     CDS             complement(638..1564)
                     /locus_tag="VGM46_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019856973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEY4192268.1"
                     /translation="MVTRLYTHPIFLEHITPPGHPERPDRLRAIERALDDEVFERLDR
                     IQAPIGDEATILYAHPAEFVDRVRAAVPEIGIARIDADTTASPKSWQAAVTAIGAANA
                     AVDDVFESRADNVFVAARPPGHHAEKTTAMGFCLFNTAAIAARYAQKKHGAERVAIVD
                     WDVHHGNGTQDIFWDDPSVLYCSTHQMPLYPGTGAKDETGAGNIVNAPLEPRTGSDHF
                     RDAFLSRVLPALDDFRPDLIIVSAGFDAHHRDPLAEINLTEDDFDWATGLLMERAARH
                     SANRLVSLLEGGYDLQGLAFSVAAHVGRLMKG"
     gene            complement(1650..2750)
                     /gene="ribB"
                     /locus_tag="VGM46_06450"
     CDS             complement(1650..2750)
                     /gene="ribB"
                     /locus_tag="VGM46_06450"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003532339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEY4192269.1"
                     /translation="MPYDQKKIVEALRAFEQGEIVVVMDDDGRENEGDLIVAAVHCTP
                     EKMAFIIRNTSGIVCTPMPRAEAKRLNLAPMVADNDSAHTTAFTVSVDFKHGTTTGIS
                     AEDRTLTVRNLANGNVGGGDFLRPGHIFPLIAREGGVLMRSGHTEAAVDLCKLAGLPL
                     VGVISELVNDDGTVKRGPQVKAFAEEHGLKQVSVADLIAYRQRQETLIERVASSDIDT
                     PGGKAKVFTYTLPWDVMQHLAVVFGDIRDGEEVPVRLHSEDVVTDVFGTDHRLDAIMK
                     LMGERQRGVIVYLREGSVGVAHQDRKRPATEDREDHEEARRRESEWREIGLGAQILKD
                     LGISSINLIASRERHYIGLEGFGIRIANTEIL"
     gene            complement(2791..3891)
                     /gene="aroC"
                     /locus_tag="VGM46_06455"
     CDS             complement(2791..3891)
                     /gene="aroC"
                     /locus_tag="VGM46_06455"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007009839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEY4192270.1"
                     /translation="MSHNTFGHLFRVTTWGESHGPALGCVVDGCPPGIRFTVADIQAE
                     LDKRRPGQSRFVTQRREPDEVRVLSGTILDEDGETLITTGTPVSMMIENVDQRSKDYG
                     EIARQYRPGHADYAYDMKYGLRDHRGGGRSSARETAARVAAGALARKVVPGMAVRGAL
                     VSMGEKAIDRANWNWNFIDDAQNPFFTPDPASVSVFADYLDGIRKEGSSVGAVIEIVA
                     DGVPAGLGAPIYAKLDQDIASNLMSINAVKGVEIGNGFEAARITGQENADEMRIGNDG
                     KPVFLSNNAGGILGGISTGQQIVARFAVKPTSSILTPRKSIDKDGNDVDVVTKGRHDP
                     CVGIRAVPIGEAMVACALADHYLRHRGQTGRI"
     gene            4070..4333
                     /locus_tag="VGM46_06460"
     CDS             4070..4333
                     /locus_tag="VGM46_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1344 domain-containing protein"
                     /protein_id="HEY4192271.1"
                     /translation="MRTLFSAVAAMLLVSTAAFAGQTEGLIKKIDKNTLTMTLDDGKS
                     YKLNAETDIDSLKEGMDVVIAFDVTDGENVVTDMELPDSDSAN"
     gene            complement(4435..5022)
                     /locus_tag="VGM46_06465"
     CDS             complement(4435..5022)
                     /locus_tag="VGM46_06465"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY4192272.1"
                     /translation="MSPLVYFIRHGQTDWNAENRLQGQADIDLNALGRRQATENGARL
                     AGLIDNPDHFDFVASPMKRTRETMERARAAMGIDPHVYRTDARLVEVNFGDWQGFTYT
                     ELEARHPGISSERSLDKWDYRPSGEGAESYQMLLERIKPWFEELARDTVCVTHGGVMR
                     ALFRLIEKVPKDTAASLDTPQDRLLRLRDGHLQWL"
     gene            complement(5065..5883)
                     /gene="fabI"
                     /locus_tag="VGM46_06470"
     CDS             complement(5065..5883)
                     /gene="fabI"
                     /locus_tag="VGM46_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007703652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEY4192273.1"
                     /translation="MAGGQGLMAGKRGLILGVANNRSIAYGIARACVDQGAEVALTYQ
                     GEAFKKRVEPLAAELKAHVCGHCDVTDPASLDAVFADVAAHFGKIDFLVHAIAFSDKE
                     ELDGRYVDTTRANFARTMEISVFSFTDIAKRAETLMPDGGSLLTLTYYGAEKVMPHYN
                     VMGVAKAALEASVRYLAVDLGGKGIRVNAISAGPIKTLAASGIGDFRYILKWNEYNSP
                     LKRTVTPEEVGDSGLYLLSDLSRGVTGEVHHVDSGYHVVGMKAVDAPDISTVKD"
     gene            complement(5986..6930)
                     /locus_tag="VGM46_06475"
     CDS             complement(5986..6930)
                     /locus_tag="VGM46_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018426786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEY4192274.1"
                     /translation="MRDPYEVLGVAKSASAKEIKSAYRKLAKKYHPDQNPDDPKAKDR
                     FAAANQAYEVVGDEKNRAAFDRGEIDGEGKPKFQGFEGAAGGGDPFAGFRRQQQGPGS
                     AHFEFRQGRPGGDPFDGNSDIFSQIFGDAFAQQRTAGAGRAQQQQAPAGEDVSVTLDI
                     TVDEAATAPKVTAMFPGGRKIAVKLPTYVEDGQTIRLKGQGEPSPYGGKAGDALVKIR
                     FRKHPRYRVEGRDLHADLPVSLKDAVLGAKVAVETPSGRLALTIPAWSSSDKVLRLKG
                     RGLPVKTGGHADLYAHVQIMLPEGGEPQLEALLRGQND"
     gene            complement(7030..7491)
                     /locus_tag="VGM46_06480"
     CDS             complement(7030..7491)
                     /locus_tag="VGM46_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006330537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RT0821/Lpp0805 family surface protein"
                     /protein_id="HEY4192275.1"
                     /translation="MSRVAQAFYSLPASVFVSASLKTAILGLTLAVAGCGAGGFSLDQ
                     ADVDRSIVTSNVPSTPPNEDTNLVADQATIRNAISSADVQTLAGKDIPWANTETGSRG
                     AVTQLAEDKSGGQLCRRFTVSRESFDGVSLFKGEACMVGYGAWRMEAFTAL"
     gene            7531..8148
                     /gene="pdxH"
                     /locus_tag="VGM46_06485"
     CDS             7531..8148
                     /gene="pdxH"
                     /locus_tag="VGM46_06485"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010914860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEY4192276.1"
                     /translation="MNETALTDDDFTESMEPFRLFARWLEDATKSEPNDPNAVALASV
                     DADGLPDVRMVLLKGFDERGFVFYTNLESAKGREILGSMKAAMCFHWKSLRRQVRVRG
                     PVEVVSDAEADEYYATRPRGSRIGAWASKQSRPLESRFALEKAVAEYTARHAIGAIPR
                     PQHWSGFRIVPSHLEFWHDRPFRLHDRVVFDRAEDGGWNKTRLYP"
     gene            complement(8330..>8689)
                     /locus_tag="VGM46_06490"
     CDS             complement(8330..>8689)
                     /locus_tag="VGM46_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY4192277.1"
                     /translation="DLGLVPEAGSSLLAPQILGLQGAFALLALGEGFSAERARAAGLI
                     HEVVEENSLEASVLAAAAQIAAKPPEALKIARDLMRSPRGELAERIRLEAEHFRERLT
                     SNEARAALTAFMSRKKG"
BASE COUNT         1569 a         2716 c         2739 g         1665 t
ORIGIN      
        1 ccaatgcgat ccggtataag ccttggctgg gtcaatgccc aaatggccac atgggtgtcg
       61 agcagtatcc tcaaggttta tccacgccga acatctcggc aatttcggcg tccgtcgaat
      121 tccattcttc aagcgtaaac ggcggatact caccttcgaa cataccgagc ttgcgtccgg
      181 tcttcttgac agtctgtacc ggcaccagtc gcgccgccgg cttgccgttg cgggcgatga
      241 tgatttcgct ctccgcgccg ctttcgaccg cctccaccag acgcgagagc gaggtctttg
      301 catccagcat gttgacgaca ggcattgttt agcctcctta gctaatcctg gctaagataa
      361 cgccgcgagc acgaaaatac aaattattcc gcgtccagcg gttccgtgcc ggccggcttg
      421 ccgtcgcggg aaaggcgaat cttttcaacc ttgtcttcgg cggccttcaa caagcggtcg
      481 caatgcgcct tcagcgcctc gccgcgctca tagatgcgga tcgactggtc tagcggcacg
      541 tcgccgcgct ccagatcgtc gacgatcttc tccagcgcat cgagcgcctg ctcgaagctc
      601 atcgccttca catcttcatt cgcttcgtca gccatgctca tcctttcatc agccgcccga
      661 catgggcggc aaccgaaaac gccagtccct gtagatcgta gccgccctca agcaggctga
      721 cgaggcggtt ggcgctgtgg cgcgccgcgc gctccatcag cagccccgtc gcccagtcga
      781 aatcatcttc ggtcaggttg atctcggcca gcggatcgcg gtggtgcgcg tcgaacccgg
      841 ccgagacgat tatcaggtcc ggccggaaat cgtccagcgc cggcaagacg cgcgacagga
      901 aagcgtcgcg gaaatgatcg ctgccggtgc gaggttcaag cggggcgttg acgatgttgc
      961 cggcgcccgt ctcatccttg gcgccggtgc cgggataaag cggcatctgg tgcgtggaac
     1021 aataaagcac cgaaggatcg tcccagaaga tatcctgcgt gccgttgccg tgatgcacgt
     1081 cccagtcgac gatggccacc cgctcggcgc catgcttttt ctgcgcgtaa cgcgcggcaa
     1141 tcgctgccgt gttgaacagg caaaagccca ttgccgtggt cttttcggca tgatggccgg
     1201 gcgggcgggc ggccacgaaa acattgtcgg cacggctttc gaacacatcg tccaccgccg
     1261 cattggcagc gccgatcgcc gtcacggcgg cctgccagct cttcgggctg gcggtggtgt
     1321 cggcgtcgat gcgggcgatg ccgatttcgg gcacggcggc gcggaccctg tccacgaact
     1381 cggccgggtg cgcatagaga atggtcgctt cgtcgccgat gggcgcctgg atgcggtcga
     1441 ggcgctcaaa gacttcatcg tccagcgcgc gctcgatggc acgcaggcgg tcgggccgct
     1501 cgggatgtcc gggcggcgtg atgtgttcga ggaagatcgg gtgggtataa agcctggtga
     1561 ccatgccccg atataggccc gcacggccta tttggccacc tttgcattcc cgcaaaggct
     1621 ggccgttaac aggcgaattt gggccgaagt cagagtatct cggtgttggc gatgcggatg
     1681 ccgaagcctt cgaggccgat ataatggcgc tcacgcgagg caatcaggtt gatcgaggaa
     1741 atgcccagat ccttcaggat ctgtgcgccg aggccgatct cgcgccattc gctttcgcgc
     1801 cggcgcgctt cctcatggtc ctcgcggtct tcggtggccg gacgcttgcg gtcctggtgc
     1861 gcgacgccga ccgaaccttc gcgcagatag acgatgacgc cgcgctggcg ctcgcccatc
     1921 aatttcatga tcgcgtcgag ccggtggtcg gtaccgaata cgtcggtcac cacatcctcg
     1981 gaatgcaggc gcaccggcac ttcctcgccg tcgcgaatat cgccgaagac caccgccagg
     2041 tgctgcatga cgtcccatgg cagggtgtag gtgaacacct tggccttgcc gccgggagtg
     2101 tcgatatcgg aactggcgac acgctcgatc agcgtttcct ggcgctggcg gtaggcgatg
     2161 agatcggcaa ccgaaacctg cttgaggccg tgttcttcgg cgaaagcctt cacctgcggt
     2221 ccgcgcttga cggtgccatc atcattgacc agttcggaaa tgacgccgac cagcggcagc
     2281 ccggcaagct tgcacaggtc gacggcagcc tcggtatggc cggagcgcat cagaacaccg
     2341 ccctcgcgcg caatcagcgg gaaaatatgg cctgggcgca ggaaatcgcc gccgccgaca
     2401 ttgccattgg ccaagttgcg caccgtcagc gtgcggtctt cggctgaaat gccagtggtt
     2461 gtgccatgct tgaagtcgac gctgaccgta aaggcggtgg tgtgggccga atcattatcg
     2521 gcgaccatcg gcgcaaggtt gagccgtttg gcttcggcgc gcggcatcgg cgtgcagacg
     2581 atgccggagg tgttgcggat gatgaacgcc atcttttccg gcgtgcagtg aacggcggcg
     2641 acaatcaggt caccctcgtt ttcgcgcccg tcatcgtcca tgaccacgac gatctcgccc
     2701 tgttcgaagg cgcgcagggc ttcgacgatt ttcttctggt cgtaaggcat cggtgaggtg
     2761 tccgttcgaa acgatggatt tcaggaaaat tcagatgcgc ccggtctgcc cgcggtgccg
     2821 aagatagtgg tcggcaagcg cgcaggcgac catcgcctcg ccgatcggca cggcgcggat
     2881 gcccacgcac ggatcgtgcc gccccttggt gacgacatcg acgtcattgc cgtccttgtc
     2941 gatggatttt cgcggcgtga ggatggacga ggtcggcttg acggcgaagc gggcgacgat
     3001 ctgctggccg gtggaaatgc cgcccagaat gccgccggca ttgttggaca ggaacacggg
     3061 cttgccgtca ttgccgatgc gcatttcatc ggcgttctcc tggcccgtaa tgcgggccgc
     3121 ctcgaagccg ttgccgatct cgacgccctt gaccgcgttg atcgacatca aattggaagc
     3181 gatatcctga tcgagcttgg cgtagatcgg cgcgccaagc cccgctggaa ctccatccgc
     3241 cacgatctcg atgaccgcgc cgaccgagga accttccttg cggatgccgt cgagataatc
     3301 ggcgaagacg cttaccgatg cgggatccgg cgtgaagaac ggattttgcg cgtcatcgat
     3361 gaaattccag ttccagttgg cccggtcgat ggctttttcg cccatcgaaa ccagcgcgcc
     3421 gcgcacggcc atgccgggca ccaccttgcg cgccagcgcg ccggccgcca cgcgcgccgc
     3481 cgtttcgcgg gcggaggaac ggccgccgcc gcgatggtcg cgcaggccgt atttcatgtc
     3541 ataggcatag tcggcatggc cgggacgata ctggcgggcg atctcgccat aatccttgga
     3601 gcgctggtcg acgttctcga tcatcatcga aaccggcgtg ccggtggtga tcaacgtctc
     3661 gccgtcttcg tcgagaattg tgccggaaag aaccctcacc tcgtccggct cgcggcgctg
     3721 ggtgacgaaa cgcgactggc cggggcggcg cttgtccagt tccgcctgaa tgtcggcaac
     3781 ggtaaagcgg atgccgggcg ggcagccatc caccacgcag ccaagggccg ggccatggct
     3841 ttcgccccag gtggtaacgc ggaaaagatg gccgaacgta ttgtgcgaca tggaaatcta
     3901 gccctcgcca gcctgcggcc ggcacgtcgc tgccttctat ttgcgtttta agcgggggtc
     3961 aaactgtcgc tttgcggccc aagctttgac acattggctt ggtttctgct ttcctcgatc
     4021 cgcccggcaa gatcgggcgg cccgacgcaa tagaagagag agcacccata tgcgtaccct
     4081 gtttagcgcc gtcgccgcca tgttgcttgt ttcgaccgca gcctttgcgg gccagaccga
     4141 gggattgatc aagaagatcg acaagaacac cctgacgatg accctggacg acggcaaatc
     4201 ctacaaattg aatgccgaaa ccgacatcga ttcgctgaag gaaggcatgg acgtggtcat
     4261 cgccttcgat gtgacggatg gcgaaaacgt tgtgaccgac atggaattgc ccgacagcga
     4321 ttcggcgaac tagagcggtt ctggttttta tcgaaatcga caaaaccgct ctatttcttt
     4381 gttttgaccg catttccgga cgcaaaaccg tgctggaaat gctttagcga ccttctacag
     4441 ccactgaaga tggccgtcgc gcagcctcag cagacggtcc tgcggggtgt cgaggctggc
     4501 ggcggtgtcc ttcgggactt tttcgatcag gcgaaacaat gctcgcatga cgccgccatg
     4561 cgtgacgcaa accgtgtcgc gggcaagttc ctcgaaccac ggctttatcc gctcaagcag
     4621 catctggtag ctttccgcgc cctcgcctga aggccggtag tcccatttgt cgaggctgcg
     4681 ttcactcgat atgccgggat ggcgcgcttc cagttcggta taggtgaagc cctgccaatc
     4741 gccgaaattg acttcgacca gtcgggcatc ggtgcggtag acgtgcgggt cgatccccat
     4801 tgcggcgcgc gcacgctcca tggtttcgcg cgtgcgcttc atcgggctgg cgacgaaatc
     4861 gaaatggtca gggttgtcga tcagcccggc gaggcgtgcg ccgttttcgg tcgcctgcct
     4921 gcgccccaaa gcgttcaggt cgatgtcggc ctgcccttgc agccggttct cggcgttcca
     4981 gtcggtctgg ccgtggcgga tgaaatagac aagcggcgac atggcgtccg agcggctcca
     5041 aacaacaggc aaggcgggag ggcgtcagtc cttgacggtc gagatatcgg gcgcgtccac
     5101 cgccttcatg ccgacgacat ggtagccgga atcgacatgg tggacttcgc cggtgacgcc
     5161 gcgcgacagg tctgacagca gatatagccc cgaatcgccg acttcttcgg gcgttacggt
     5221 gcgcttgagc ggcgaattat attcgttcca cttcaggata tagcggaaat cgccgatgcc
     5281 ggaggcggcc agcgtcttga tcggccccgc cgagatggcg ttgacgcgga tgcccttgcc
     5341 gccgagatcg acagccagat agcgcacgct ggcttccagc gccgccttgg cgacgcccat
     5401 gacgttatag tgcggcatca ctttttcagc gccgtaatag gtcagcgtca acagcgagcc
     5461 gccatccggc atcagcgttt cggcgcgctt ggcgatgtcg gtgaaggaaa agacggagat
     5521 ttccatggtg cgcgcgaagt tggcgcgggt cgtatcgaca taacggccat cgagttcctc
     5581 cttgtcggag aaggcgattg cgtggacgag gaaatcgatc ttgccgaaat gcgcggcaac
     5641 atcggcgaac acggcatcga ggcttgccgg atcggtcacg tcgcaatggc cgcaaacatg
     5701 cgccttgagt tcggcggcca gcggttcgac gcgcttcttg aacgcctcgc cctgataagt
     5761 cagcgcgacc tccgcgccct ggtcgacgca ggccctggca atgccgtagg caatcgatcg
     5821 attattggca acgcccagga taaggccgcg tttgccggcc atcaggccct gtccacccgc
     5881 catgtgcgcg ctcctcgcat attctagatg cgggagccta tcgcacaggc gcacagcgcc
     5941 ttcaagcgct caaacgaaca gctttggccg agaaaacggc atgatctaat cgttttgccc
     6001 gcgcagcaag gcctcaagct gcggctcgcc gccttccggc agcatgatct gcacatgcgc
     6061 gtagaggtcg gcatggccgc ccgtcttcac cggcaagccc ctgcccttca gccgcagcac
     6121 cttgtcggaa ctggaccatg cggggatggt cagcgccagc cggccggacg gcgtttccac
     6181 cgcaaccttg gcgcccagca ccgcatcctt cagcgaaacc ggcaggtcgg catgcaggtc
     6241 ccggccctcg acgcggtagc gcggatgctt gcggaaccgg atcttcacca gcgcgtcgcc
     6301 cgccttgcca ccataaggcg aaggctcacc ctggcctttc aggcgaatgg tctggccgtc
     6361 ctcgacatag gtgggcagct tgaccgcgat cttgcggccg ccgggaaaca tcgccgtcac
     6421 cttgggcgcg gtcgcggcct cgtcgacggt gatgtcgagc gtgacggaaa catcctcgcc
     6481 tgccggtgcc tgctgctgct gcgcgcggcc agcgccggcc gtacgctgct gggcgaaggc
     6541 atcgccgaat atctggctga aaatgtcgct gttgccgtcg aacggatcgc cgccgggccg
     6601 gccctggcgg aattcgaaat gcgcgctacc cggcccctgc tgctgacgcc ggaaaccggc
     6661 aaacgggtcg ccgccgccgg cagcgccctc aaagccctgg aatttcggct tgccttcccc
     6721 gtcgatttcg ccacggtcga aggcggcgcg gttcttttcg tcgcccacga cctcataggc
     6781 ctggttggcc gcggcaaagc ggtccttcgc cttgggatcg tccgggttct ggtccggatg
     6841 atatttcttg gcgagcttgc ggtaagccga ttttatttcc ttggccgagg cgcttttggc
     6901 aacgcccagc acctcatagg ggtcgcgcat atggaacatc ccgaactgaa ggcagattca
     6961 caattgtcta ctatatgcgt tcacgtagga agaaattcca gtatgcgacg cggcttcaag
     7021 gacaaaatct cacaaggccg tgaaggcttc catccgccac gcgccgtagc cgaccatgca
     7081 ggcctcgccc ttgaacagcg aaacgccgtc gaagctttcc cgcgatacgg tgaagcgccg
     7141 gcaaagctgg ccgccggact tatcctcggc cagctgggtg accgcgccgc gcgagcctgt
     7201 ttcggtattc gcccacggga tatccttgcc ggccagcgtc tgcacatcgg ccgacgaaat
     7261 cgcgttgcgg atggtcgcct ggtcggcgac gaggttggta tcctcgttcg gcggcgttga
     7321 cggcacgttg ctggtgacga tggagcgatc gacatcggcc tggtccaggc tgaagccgcc
     7381 ggcgccgcag ccggcgacgg ccagcgtaag gcccagtatt gcggtcttca ggctggccga
     7441 aacgaaaacg ctcgcaggca ggctgtaaaa tgcttgcgcg acgcgcgaca atcggcaatc
     7501 tcccgccaat ggatagagtt tgaaaaaact atgaacgaaa ccgcgttaac agatgatgac
     7561 tttaccgaga gcatggagcc ttttcggctt tttgcgcgct ggctggagga cgccaccaaa
     7621 tccgagccga acgaccccaa cgcggtggcg ctggccagcg tcgatgcgga cggcctgccg
     7681 gacgtgcgca tggtgctgtt gaaggggttc gatgagcgcg gtttcgtctt ctacaccaat
     7741 ctggaaagcg ccaaggggcg cgaaatactg ggcagcatga aggcggcgat gtgctttcac
     7801 tggaaatcgc tgcgccggca ggtgcgggtg cgcggtcccg tcgaggtcgt cagcgatgcc
     7861 gaggccgacg aatattacgc aacgcgcccg cgcggcagcc ggatcggcgc atgggcgtcc
     7921 aagcagtcgc gcccgctgga aagccgtttc gcgctggaaa aagcggtcgc cgaatatacc
     7981 gcgcgccacg ccatcggcgc gatcccgcgc ccgcaacact ggtccgggtt ccgcatcgtg
     8041 ccgagccatc tcgaattctg gcacgaccgc ccgttccggc tgcatgaccg cgtggttttc
     8101 gaccgggcgg aagacggcgg gtggaacaag acgcgtcttt atccataagg cggcgcagcc
     8161 cctccgcctc gctacgctcg gcacctctcc ccgcaagcgg ggcgaggaaa gcggtggtgc
     8221 gacgggtctt gctcgcctcc atcatgtcgg gaaagtatcc gcgaagcgga cggagggggt
     8281 caattccttc gcggccgccg ccgcggctgc ggcattcgcc ggccgctctt taccccttct
     8341 tgcggctcat gaaggccgtc agggcggcgc gggcttcatt ggaggtgagg cgctcgcgga
     8401 aatgctcggc ttccagcctg atgcgctcgg caagttcccc gcgcgggctg cgcatcaggt
     8461 cgcgggcgat cttcaacgcc tccggcggct tggcggcgat ctgggcggcg gcggccagaa
     8521 cggaggcttc cagcgaattt tcctcgacca cctcgtgaat cagcccggcg gccctggctc
     8581 gttcggccga aaatccttca ccgagcgcga gcagcgcgaa agcgccttga agcccgagga
     8641 tttgcggcgc cagcaggctg gagcctgctt ccggcacaag cccaagatc
//
LOCUS       DASYCS010000200         4201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1643374,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000200 DASYCS010000000
VERSION     DASYCS010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4201
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1643374"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1502)
                     /locus_tag="VGM46_06495"
     CDS             complement(<1..1502)
                     /locus_tag="VGM46_06495"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532020.1"
                     /note="Catalyzes the rate-limiting step in dNTP synthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HEY4192278.1"
                     /translation="MRIDRRFTKNGHAGQERAAYAEIEFRKALSEIKNPDGSVVFRLD
                     NIDVPAQFSQVAADILAQKYFRKAGVPARLKKIEENDVPSFLWRSVADEAELAKLPEA
                     ERYGSETDARQVFDRLSGTWTYWGWKGGYFKAEEDARAFRDELAYMLATQRVAPNSPQ
                     WFNTGLHWAYGIDGPGQGHFYVDPFTGKLTKSKSSYEHPQPHACFIQGVQDDLVNEGG
                     IMDLWVREARLFKYGSGTGSNFSMLRGEGERLSGGGRSSGLMSFLKIGDRAAGAIKSG
                     GTTRRAAKMVIVDADHPDIEAFIDWKVKEEQKVASLVTGSKIVRKHLDAIMKACVACE
                     GEGDDCFDPSINTALKRAIKAAKKDAVPENYVQRVIQFARQGYTSMDFKTYDTDWDSE
                     AYLTVSGQNSNNSVSLKDDFLRAVEQDGDWNLTARKDGRVMKTMKARDLWEKIGYAAW
                     ASADPGLHFNTTMNDWHTSPAAGPIRGSNPCSEYMFLDDTACNLASINLLP"
     gene            complement(2242..2733)
                     /locus_tag="VGM46_06500"
     CDS             complement(2242..2733)
                     /locus_tag="VGM46_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019857844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY4192279.1"
                     /translation="MLTGPQTHEHLAERVRKLFGRVPCRLQVAALPWRRIDGNIEIML
                     VTSRDTRRWVLPKGWPEAREPLCTAAAREASEEAGLSGRISHLEVGRYFYAKALASGE
                     EVPCEVLVFPLQVDAVAQRWKEKHSRTRKWVAASEAPTMVDEPDLSQIIAFFGVNADR
                     FDS"
     gene            complement(2883..3383)
                     /gene="gpt"
                     /locus_tag="VGM46_06505"
     CDS             complement(2883..3383)
                     /gene="gpt"
                     /locus_tag="VGM46_06505"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012092033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEY4192280.1"
                     /translation="MSLPEKAFPVSWDQFHRDARALAWRLAGTNKGQWRAIVCITRGG
                     LVPAAIISRELGIRVIETVCVASYHDYNSQGQLQVLKEVTPSLLADDGEGVLIIDDLT
                     DTGKTAAIVRAMMPKAHFAAVYAKPKGRPMVDTFVTEVSQDTWIYFPWDMGFTYQKPI
                     ADDHAG"
     gene            complement(3517..4077)
                     /locus_tag="VGM46_06510"
     CDS             complement(3517..4077)
                     /locus_tag="VGM46_06510"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY4192281.1"
                     /translation="MPRPDFDAQPSLGNDTLRLRPLRREDLEDLFAAAGRPQVWAGHP
                     AKDRYKRDVFEGYFDFLLETASALAVIDPHTSKIIGSSSYYVAPDRPDGISIGFTFLN
                     DEYWGGATNFELKRLMLDHAFKTFPEVWFHIGPSNVRSQKATAKLGAIHAYDATLNLG
                     GSPALWMNFCLSKAAWEATLARKEQA"
BASE COUNT          825 a         1267 c         1267 g          842 t
ORIGIN      
        1 ggcagcaggt tgatggaggc caggttgcag gccgtatcgt cgaggaacat atattccgag
       61 cacgggttgg agccgcggat aggcccggcc gccggcgagg tgtgccagtc gttcatcgtc
      121 gtgttgaaat gcaggccggg gtcggccgag gcccaggcgg cgtagccgat cttctcccac
      181 aggtcgcgcg ccttcatggt cttcatgacg cggccgtcct tgcgggcggt caggttccag
      241 tcgccatcct gctcgacggc gcgcaggaag tcgtccttca gcgaaaccga attgttggag
      301 ttctggccgg aaaccgtgag ataggcctcc gaatcccagt cggtgtcgta ggtcttgaaa
      361 tccatcgagg tgtagccctg ccgcgcgaac tggatgacgc gctggacata gttctcgggc
      421 acggcgtcct tcttggccgc cttgatggcg cgcttcaggg cggtgttgat cgacgggtcg
      481 aagcagtcgt cgccttcgcc ttcgcaggcc acgcaggcct tcatgatggc gtcgaggtgc
      541 ttgcgcacga tcttggagcc ggtaaccagc gaggcgacct tctgctcttc cttcaccttc
      601 cagtcgatga aggcttcgat atcgggatgg tcggcatcga cgatgaccat cttggcggcg
      661 cggcgcgtcg tgccgccgga cttgatggcg ccggcggcgc ggtcgccgat cttgaggaag
      721 gacatcagcc ccgacgaacg gccgccgccg gaaagcctct cgccttcgcc gcgcagcatc
      781 gagaagttgg agccggtgcc ggaaccgtat ttgaacaggc gcgcctcgcg cacccacaaa
      841 tccatgatgc cgccctcgtt gacgagatcg tcctgcacgc cctggatgaa gcaggcatgc
      901 ggctgcggat gctcgtacga agacttggac ttggtcagct tgccggtgaa gggatcgacg
      961 tagaaatggc cctggccggg gccgtcgatg ccataggccc aatgcaggcc ggtgttgaac
     1021 cattgcggcg aattcggcgc gacgcgctgg gtggcgagca tgtaggcaag ctcgtcgcgg
     1081 aaggcgcggg cgtcttcctc ggccttgaag tagccgccct tccagcccca ataggtccag
     1141 gtgccggaga gacggtcgaa cacctggcgc gcgtcggttt ccgaaccgta gcgctcggct
     1201 tccggcaact tggcaagctc ggcctcgtca gccacggagc gccacaggaa ggaggggacg
     1261 tcgttctcct cgatcttctt caggcgtgcc ggcacgccgg ccttgcggaa atatttctgg
     1321 gccaggatgt cggccgcgac ctgggagaac tgcgccggca catcgatgtt gtcgaggcgg
     1381 aacaccaccg agccgtccgg attcttgatt tcggaaagcg ccttgcgaaa ttcgatctcc
     1441 gcataggctg ccctttcctg gccagcatgt ccgttcttgg tgaaacgccg gtcgatgcgc
     1501 attggtcccg ttcctttgcc tgatctctat atatagcact tttcgaggag gatttccgcc
     1561 ctcaattcga aacgcgcatg tcaaccgccc gccaggcgct ccgaagatcg ccggcagtcc
     1621 ccttcgccgt tttcgccaca atgcctggcg gagcttttct ggaaagcgcc gctggcttgc
     1681 cggcaacccc gccgaccctc atgctgaaac ttttgacgat tctctcattc gagagaactc
     1741 actctatcta gtgccgaggc tgtcatcaaa cactaaatat agtgttaaca gacaatttat
     1801 tccagcccgg caattttccc tttcgtcccg ttggaaacca aaaattccgc gcatccgcca
     1861 cccgcgtaaa cggagaaaat gcggctaaaa cgcatgaaat cgggaaaaag gaccggcctc
     1921 aaaactttcc ggtcacgctc tcaacaaaag cgcatggtcc ataaaaggcg actcggatag
     1981 cgccgtcaag agttcgtttc tgtaactttt atgaccccaa catattgtgt gccacttatg
     2041 tggataacgg ggatagaaat gaacctgcgt cgtttgtggt tgtgtcgcgg ccggacttgg
     2101 tgggcgcgca aaatcttgcc cccaccggcg cacggctatc gaaaatggcg agatcaacgg
     2161 catgaaggct atggccggca tgcttcgtgg ccggccagca tgtccaccct tcatgatgtg
     2221 cggtgaggcc tgcggcttgt cttagctgtc gaagcggtct gcattcacgc cgaaaaaggc
     2281 gatgatctgg ctaaggtcgg gttcgtcgac catggttggg gcctcgctgg ccgcaaccca
     2341 tttgcgcgta cgcgaatgct tttccttcca gcgttgcgcc accgcatcga cctgaagcgg
     2401 aaacaccagc acctcgcagg gcacttcttc gcccgaggca agcgccttgg cgtaaaaata
     2461 ccttccgact tcaagatggg aaatcctgcc gctcaggccg gcttcttccg atgcctcgcg
     2521 ggccgccgcc gtgcaaagcg gttcgcgcgc ttcgggccaa cccttcggca aaacccagcg
     2581 gcgcgtatcg cggctggtaa ccagcatgat ctcgatattt ccgtcgatcc ggcgccaggg
     2641 cagggcggcg acctgtaggc gacaaggcac cctgccgaag agcttgcgca cccgctcggc
     2701 cagatgttcg tgtgtctgtg gcccggtcag cacctgaagc catacccctt caatgccaga
     2761 atcgtttgcc gtcttacgaa tcttacggaa tatcaggcgc aataaaagta aaaatcacgc
     2821 aaaatccgca caaaacgaaa aagcggccaa ggccgccttt gtttatgggc agggtggctg
     2881 cttcagccgg cgtggtcgtc ggcaatcggc ttttggtagg taaagcccat gtcccagggg
     2941 aagtagatcc acgtatcctg gctgacttcg gtgacgaagg tgtcgaccat cgggcggccc
     3001 ttgggcttgg catagaccgc cgcgaaatgc gcctttggca tcatggcgcg cacgatcgcc
     3061 gccgtcttgc cggtgtcggt caggtcgtcg atgatcagaa cgccttctcc gtcgtcggcc
     3121 agcagcgaag gggtaacctc cttcagcacc tgcaactgcc cctggctgtt ataatcgtgg
     3181 taggaggcga cgcatacggt ttcgatgacg cggatgccca gttcccgcga aatgatcgcg
     3241 gccggcacca gtccgccacg cgtaatgcac acgattgccc gccattgtcc cttgttggtt
     3301 ccggccagcc gccatgccag cgcgcgcgca tcgcggtgga actggtccca ggaaacggga
     3361 aaggcttttt cgggaaggga cattttcgcg cactccagcg cgaaaccgcg cgatactgaa
     3421 aggaatgcgg gcggaactag ccggaaaacc ggggccatgg caagcgcagt ttagcaaaag
     3481 cgcaaaacca tgccgggcgg ccaacagttt tctggtctac gcctgctctt tcctggcaag
     3541 ggtcgcttcc cacgcggctt tgctcaggca aaaattcatc cacagcgccg gcgaaccgcc
     3601 caggttcagc gtcgcatcat aggcgtggat tgcgccgagc tttgcggtgg ctttctgcga
     3661 gcgcacattg ctcggtccga tgtggaacca gacttcggga aatgtcttga aggcatggtc
     3721 cagcatcagg cgcttcagtt cgaaattcgt tgcgccgccc caatattcat cgttcaggaa
     3781 agtgaagccg atcgatatgc cgtccggccg gtcgggagcc acataatagc tggaagagcc
     3841 gatgattttc gacgtatgcg ggtcgatgac ggcaagcgcc gacgcggttt cgagcaggaa
     3901 atcgaaatag ccctcgaaaa cgtccctttt atacctgtcc ttggcagggt gcccggccca
     3961 gacttggggg cgtccggccg ctgcgaacaa atcctccaaa tcctcgcgcc gcaaagggcg
     4021 caggcgaagc gtgtcattgc cgagcgacgg ctgtgcatcg aaatccggtc gcggcatatt
     4081 cggtcatccc ttggcgcaaa acggtggcac cctatgcagg aaccgccgta ccgacttgcg
     4141 ccaaggagac gccatggcca atgccagcct tatcgcgaca ggaaggcggc aacctggccc
     4201 g
//
LOCUS       DASYCS010000201         2049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_627166,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000201 DASYCS010000000
VERSION     DASYCS010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627166"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /locus_tag="VGM46_06515"
     CDS             <1..939
                     /locus_tag="VGM46_06515"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEY4192282.1"
                     /translation="AMLRAGHRVRLWARDEETVAAINRRENRHYLPGITLDPGIEAMS
                     DMATALAGADCVLAVTPAQSLRAVLAAAKDHMPQSVPLVLCAKGIERGTGALLSSIVG
                     EILPDNPIAALSGPSFAADVAKGLPTAVVVAAQNERMAGELAARFSARNLRCYSSADL
                     VGVEIGGALKNVFAIAAGAATGAGLGASAQAAMVTRGFVELRRIGAAFGAKPETLMGL
                     SGLGDLLLTCSTPQSRNFAYGLALGRGEELAGRPLAEGVPTAGIAARIALERGIDAPI
                     ISAVAAILDGSITVGEAVSALMTRPLKTETETGDRN"
     gene            969..1265
                     /locus_tag="VGM46_06520"
     CDS             969..1265
                     /locus_tag="VGM46_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009450869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI-like protein"
                     /protein_id="HEY4192283.1"
                     /translation="MLYAVICKDKPGSLQVRMDTRPEHVAFLEGLNGEGKLAFAGPFI
                     DANGQPNGSLVVIEATDLASAKMLAASDPYAKAGLFENVEVQQWNWSFNKPANG"
     gene            1287..1727
                     /locus_tag="VGM46_06525"
     CDS             1287..1727
                     /locus_tag="VGM46_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006115716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEY4192284.1"
                     /translation="MKYWLFKSEPFKFSFDALKATGKKGTQWDGVRNYLARNNMRAMQ
                     IGDLGFFYHSNEGLEVVGIAEVCALAHHDTTTEDPRWECVDIRAVREVPRPVTLVEVK
                     ANPKLEKMALVTSMRLSVQPVTLDEWQEVCRMAGLDPAPLGPVS"
     gene            1724..>2049
                     /locus_tag="VGM46_06530"
     CDS             1724..>2049
                     /locus_tag="VGM46_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEY4192285.1"
                     /translation="MTRLSSRDAERFIAENTALIAPPHVPEIRLHLADEAHDLWKRTE
                     EELTEIGLPPPFWAFAWAGGQGLARHLLDHPETVRNRTVLDFASGSGLVAIAAAMAGA
                     SSVTAA"
BASE COUNT          345 a          680 c          692 g          332 t
ORIGIN      
        1 gccatgctgc gcgccggcca ccgggtgcgg ctgtgggcgc gcgacgaaga aaccgtcgcg
       61 gcgatcaacc ggcgcgaaaa ccggcattac ctgcccggca tcacgctcga ccccggcatc
      121 gaggcgatga gcgatatggc caccgcgctt gccggggccg attgcgtgtt ggcggtgacg
      181 ccggcgcagt cgctgcgcgc ggtgctggcg gcggcgaagg accacatgcc gcaatccgtc
      241 ccgctggtgc tgtgcgccaa gggcatcgag cgcgggacgg gcgcgctgct gtcgagcatc
      301 gttggcgaaa tcctgcccga caatccgatt gcggcacttt ccgggccgag ctttgccgcc
      361 gacgtcgcca agggcctgcc gacggcggtg gtggtcgccg cgcagaacga acgaatggcg
      421 ggcgaactgg cggcgcgctt ttccgcccgc aatttgcgct gctattcgag cgccgacctt
      481 gtcggcgtcg agatcggcgg cgcgctgaag aacgtctttg ccatcgccgc cggcgccgcg
      541 accggggccg gccttggcgc cagcgcgcag gccgccatgg tgacgcgcgg ctttgtcgaa
      601 ctgcgccgca ttggcgcggc cttcggcgcg aagcccgaga cgctgatggg cctgtcgggc
      661 cttggcgacc ttcttttgac ctgttcgacc ccgcagtctc gcaatttcgc ctatgggctg
      721 gcgctggggc gcggcgagga actggccggg cggccgctgg ccgaaggcgt gccgaccgcc
      781 ggcatcgccg cccgcatcgc cctggagcgc ggcatcgacg cgccgatcat ctcggccgtc
      841 gccgccatac tggacggttc cattaccgtc ggcgaggcgg tatcagcgct gatgacccgg
      901 cccctgaaga ccgaaaccga gaccggcgac cgaaactgaa aatggcaatc gaacagacag
      961 gagcgaacat gctttacgcg gttatttgca aggacaagcc cggcagccta caggttcgga
     1021 tggacacgcg gcccgagcat gtcgcctttc tcgaagggct gaacggcgag ggcaaactgg
     1081 cttttgccgg tccgttcatc gatgccaacg gccagcccaa cggcagcctc gtcgtcatcg
     1141 aggcgaccga tcttgcctcg gccaagatgc tggccgcttc cgatccctat gccaaggccg
     1201 gcctgtttga gaatgtcgag gtccagcagt ggaactggtc cttcaacaag ccggcgaatg
     1261 gctaatacgg gctgcgggag cgcgcaatga agtactggct gttcaagtcc gagccattca
     1321 agttttcctt cgacgcgctg aaagccaccg gcaagaaagg cacgcagtgg gacggcgtgc
     1381 gcaactatct ggcgcgcaac aacatgcgcg ccatgcagat cggcgacctc ggcttcttct
     1441 accattccaa cgaagggctt gaggtggtcg gcatcgcgga agtctgcgcg ctcgcccacc
     1501 acgacaccac gacggaagac ccgcgctggg aatgcgtcga tatccgcgcc gtgcgcgagg
     1561 tgccgcgccc ggtaacgctg gtggaggtga aggccaaccc gaagctggag aagatggcgc
     1621 ttgtcacctc gatgcggctt tccgtgcagc cggtgacgct ggacgaatgg caggaagtct
     1681 gccgcatggc cggtctcgat cccgcgccgc ttggcccggt ttcttgacgc ggctttccag
     1741 ccgggacgcc gaacggttta tcgcggaaaa caccgccctg atcgcgccgc cgcatgtgcc
     1801 cgaaatccgc ctgcatctgg ccgacgaggc gcatgatctg tggaagcgca ccgaagagga
     1861 actgaccgag atcggcctgc cgccgccctt ctgggctttc gcatgggccg gcgggcaggg
     1921 ccttgcgcgc cacctgctcg atcacccgga aacggtacgc aaccggaccg tgctcgattt
     1981 cgcctccggc tcgggcctcg tggcgatagc ggcggcgatg gccggcgcat ccagcgtcac
     2041 cgccgccga
//
LOCUS       DASYCS010000202         2681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4541351,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000202 DASYCS010000000
VERSION     DASYCS010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4541351"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..1164)
                     /gene="modC"
                     /locus_tag="VGM46_06535"
     CDS             complement(52..1164)
                     /gene="modC"
                     /locus_tag="VGM46_06535"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006335874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HEY4192286.1"
                     /translation="MTIIVDIQHQLGAFGLDARFVSSGRLTALFGPSGSGKSSLINAI
                     AGLTRPHKGRVEVEGRVLMDTEAGIFVPKYKRRVGMVFQDALLFPHMSVAHNLRFGRW
                     FTPATERYADMDGVVELLGIGALLERRPGRLSGGEKQRVAIGRALLASPKLLLMDEPL
                     ASLDDARKAEILPYIERLRDETKIPIVYVSHSIAEVARLASDVVVLAEGRVAASGPTG
                     EIMQRLDLLPAQERGEGGAVLETKVARHDDIFGMTVLSSKAGEIRVSRLDLAAGASVR
                     LRIRARDVMVATIEPKGLSALNILPGKIAVIEPAEGASVTVKIDCNGAAVMARITRQS
                     RQTLELEVGRAVFAVVKTVSFDNANTATGPRGEADG"
     gene            complement(1161..1865)
                     /gene="modB"
                     /locus_tag="VGM46_06540"
     CDS             complement(1161..1865)
                     /gene="modB"
                     /locus_tag="VGM46_06540"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HEY4192287.1"
                     /translation="MTWLLDLTPDEWNAVQLSIKVAVVAMLFSLPPGIAVALLLARGK
                     FWGKTLIDGVVHLPLILPPVVTGYLLLLAFGRRGPAGAFLAEHFGIVFSFRWTGAALA
                     CGVMGFPLMVRAIRLSVEAVDGKLEAAAATLGANPLWVFTTITLPLILPGMIAGGILS
                     FAKAMGEFGATITFVSNIPGETQTLPSAIYTFTQVPGGDEGALRLTLISIIISMGALV
                     ASELLARRIGRRMQIG"
     gene            complement(1882..2676)
                     /gene="modA"
                     /locus_tag="VGM46_06545"
     CDS             complement(1882..2676)
                     /gene="modA"
                     /locus_tag="VGM46_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006335876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY4192288.1"
                     /translation="MHKNFGLKAWAAIGGLATVMAIAVPAAHAEGKLVVFAASSMKGA
                     LDKANEACAADVGETATISYAASSALAKQIESGAPADVFISADLDWMKYLSDKKLTKA
                     DTEVKLLGNEIVLVAPKDSKAEVKVEKGFDLAGQLNGGKLAMGDFKAVPVGKYGKSAL
                     ESLGVWKSVEGSVAQAENVRAALKLVSTGEAPLGIVYQTDAAVEPGVKVVGTFPVDSH
                     PPIVYPIAQTADSKDENTPKFLKCLQSAKAADLFKAQGFTVLAPSN"
BASE COUNT          540 a          911 c          749 g          481 t
ORIGIN      
        1 aactccgtcc ggcgctattc caaaaaggca tagcgctaaa taattgccgt gtcagccgtc
       61 ggcctcgccg cgcggacccg tagccgtatt ggcattgtcg aaactcacgg tcttgaccac
      121 ggcgaacacc gcgcggccaa cttccagttc caatgtctgc cgcgattgcc gcgtgatgcg
      181 cgccatgacg gcagcgccgt tgcagtcgat cttgaccgtt acggaagcgc cctccgccgg
      241 ctcgatcacc gcgatctttc ccggcaatat gttcagtgcg ctaagccctt tcggctcgat
      301 ggttgcaacc atcacgtcgc gggcgcgaat gcgcagacgc accgaggcgc ccgccgccag
      361 atcgagtcgc gaaacgcgaa tctcgcccgc tttcgagctg agaaccgtca tgccgaagat
      421 atcgtcatgg cgcgccactt tggtttcaag cacagcgccg ccttcgcctc gctcttgcgc
      481 gggcaacagg tccagccgct gcatgatttc gccggttggg ccggaggccg cgaccctgcc
      541 ctcggccagc accaccacgt cgctcgccag ccgcgccacc tcggcaatcg aatgactgac
      601 atagacgatg ggaattttcg tctcgtcgcg cagccgttcg atatagggta ggatttcggc
      661 tttgcgcgca tcgtcgagcg aggccagagg ctcgtccatc agcagcaatt tcgggctggc
      721 cagcaatgcc cgtccaattg ccacgcgctg cttctcgccc cctgaaagcc tgcccggccg
      781 ccgttccagc aaagcgccga tgccaagcag ctcgaccacg ccatccatgt cggcataacg
      841 ctcggtggca ggcgtaaacc agcgcccgaa acgcagatta tgcgccacgc tcatatgcgg
      901 aaacaaaagc gcatcctgga agaccatgcc cacccgccgc ttgtatttcg gcacgaatat
      961 gcccgcctcc gtgtccatca gcacccggcc ttcgacctcg acgcgccctt tgtgcggccg
     1021 cgtaagtccg gcaattgcgt tgataagcga cgattttccc gagcctgaag gcccaaacaa
     1081 cgccgtcagc cgccccgaac tgacaaaccg ggcatcgagg ccgaatgcgc cgagctggtg
     1141 ctggatgtcg acaatgatgg tcatccgatc tgcatccgtc ggcctatgcg gcgcgccagc
     1201 agctccgaag ctaccagcgc gcccatggaa atgatgatgc tgataagcgt cagccgaagc
     1261 gcgccttcat cgccgcccgg cacctgcgtg aacgtataga tagccgaggg cagcgtctgc
     1321 gtttcacctg gaatattgga aacgaaagta atcgtcgcac cgaactcgcc catcgccttg
     1381 gcaaaggaaa ggatgccccc cgcgatcatg ccgggcaaaa taagcggcag cgttatcgtt
     1441 gtaaaaaccc atagcgggtt ggcgcccagc gttgcggccg ccgcttccag cttgccgtcg
     1501 accgcttcga ccgacaggcg gatcgcccgc accatcagcg gaaagcccat cacgccgcag
     1561 gcaagggccg cacccgtcca gcggaatgaa aagacgatgc cgaaatgttc ggccagaaag
     1621 gctccggccg gcccacgccg cccaaaggcg agcagcagaa gatagcccgt caccaccggc
     1681 ggcaggatca acggcagatg gaccaccccg tcgataaggg tcttgcccca gaatttgccg
     1741 cgcgcaagca gcagcgcaac ggcaatgccg ggcggcaagc tgaacagcat tgccaccacg
     1801 gctaccttga tcgacagctg gaccgcgttc cattcatccg gcgtgaggtc cagcaaccag
     1861 gtcatgcttg gctatgatgt ctcagttgga aggcgccaga accgtgaacc cctgcgcctt
     1921 gaaaagatcg gccgccttgg ccgactgcaa gcacttcagg aacttcggcg tattctcatc
     1981 cttggaatcc gccgtctgcg cgatcggata gacgatcggc ggatgcgaat ccacagggaa
     2041 agtgccgact accttcacgc ccggctcgac cgccgcatcc gtctggtaga cgatgccgag
     2101 cggcgcttca ccagtcgaga cgagcttgag cgcggcgcgc acgttctctg cctgcgccac
     2161 gctgccctcc accgacttcc acacgccgag cgattcgagc gccgacttgc catatttgcc
     2221 gaccggcacc gccttgaagt cgcccatggc aagcttgccg ccattcagct ggccagcgag
     2281 atcgaagcct ttttcgactt tcacttcggc tttggaatcc ttgggcgcca ccagcacgat
     2341 ttcattgccg agcagcttta cctcggtgtc ggccttggtc agcttcttgt cggacaggta
     2401 cttcatccag tcgagatcgg ctgaaatgaa cacgtcggcc ggcgcgccgc tctcgatctg
     2461 cttggccagc gccgaacttg ccgcataaga gatcgtcgcg gtttcaccga catccgccgc
     2521 acaggcctcg tttgccttgt cgagcgcgcc cttcatgctg gaagccgcga aaaccaccag
     2581 cttgccctcg gcatgggccg ccggaacggc tattgccatc accgtcgcga gaccgccaat
     2641 ggcagcccaa gccttcaatc cgaaattctt gtgcatagac a
//
LOCUS       DASYCS010000203         8405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3961841,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000203 DASYCS010000000
VERSION     DASYCS010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 8405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8405
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3961841"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            337..1341
                     /locus_tag="VGM46_06550"
     CDS             337..1341
                     /locus_tag="VGM46_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016556703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HEY4192289.1"
                     /translation="MKSILSALAGVAVLASLSISPAFAQEDGIVVYNAQHESLAKEWA
                     EGFTKETGIKVTLRNGGDSEFSNQIVAEGASSPADVFLTENSPAMVLVENAGLFAPLD
                     ADTLAQVPEQFRPSTGKWTGIAARSTVFAYNKTKLKADQLPKSLLDLADPSWKGRWAA
                     SPSGADFQAIVSALLELKGEEATSAWLKAMKENFTAYKGNGTVMKAVNAGEIEGGVIY
                     HYYWFGDQAKTGENSGNVELHYFKNQDPGAFVSISGGGVLASSKHAKEAQAFVKWIAG
                     KGGQDVLKNGTSYEYAIGNGADSNPKLVPLADLQAPKVEPSKLNSKKVTDLMTAAGLL
                     "
     gene            1486..3072
                     /locus_tag="VGM46_06555"
     CDS             1486..3072
                     /locus_tag="VGM46_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEY4192290.1"
                     /translation="MQPSPHYADAAVPFGTARGRLAGRLPVSWIMLAALTVTLFALLP
                     LGFVVVVAIQSGWDTVYALVFRPRVGELLINTVLLTVVAVPICVALSIALAWLTERSD
                     LPAARLWAWLSVAPLAVPAFVHSYAWVTLVPGLHGLFAGVLVSVIAYFPFVYMPIAAS
                     LRRLDPALEDAAASLGHPPWRVFLRVVLPQLRLAICGGSLLVGLHLLAEYGLFVFIRF
                     DTFTTAIVDQFQSTFNGPAANMLAGVLVTCCLVLLAIEVLVRGEERYARVGSGAARQL
                     RVTRLGWATVPCLLLTALVAVLSLGVPFMTIGRWLIAGGTAVWQWDEIMQTLGHTLFL
                     AVAGGLLATIAAIPMAWLSIRAPGPLQRVLEGSNYVVGSLPGVVVALALVTITVRVAL
                     PLYQTLFTIVVAYALMFLPRALISLRASIAQAPIELERAAGSLGRRPINALWSTTIRL
                     SAPGAAAGMALVALGIMNELTATQMLAPNGTRTLALAFWAYSSEIDYAAAAPYALIMV
                     AVSLPLTWLLYVQSKRMAGR"
     gene            3069..4151
                     /locus_tag="VGM46_06560"
     CDS             3069..4151
                     /locus_tag="VGM46_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4192291.1"
                     /translation="MSFLEFNTIHKRYGQVAALAGVDLRVAAGTRTAIVGPSGCGKTT
                     LLRLIAGFEAPDRGRIVLDGEVLAEDGAAVPAHLRGIGLVAQDGALFPHLTIADNIGF
                     GMERRETGRDARITELAYIVGLDKAILKRRPHELSGGQQQRVALARAMARKPRLMLLD
                     EPFSALDTGLRVSMRKAVAELLETAGIATILVTHDQAEALSFADQVVVMRDGEFSQVG
                     TPRELYLKPKDRMVAEFLGDAIILPANIRGGFAQCTLGRIAVDTKETRDNARIMLRPE
                     QLLLKRTSREGMSGTPDMLFGEVVEVEFGGPVCMIAVRLVNSPEPRDAAAIGNTPLIL
                     RKPGLDAPAVGEIVRISATGKAHVFP"
     gene            4358..5338
                     /gene="ftsZ"
                     /locus_tag="VGM46_06565"
     CDS             4358..5338
                     /gene="ftsZ"
                     /locus_tag="VGM46_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008877734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEY4192292.1"
                     /translation="MNTKPRPEIAEMRPKISVIGVGGGGGNAVNNMISKSLAGAEFIV
                     ANTDAQALTMSKASRLIQLGVHVTEGLGAGSLPEVGQAAAVESIDEIMDHLTGTHMCF
                     VTAGMGGGTGTGAAPVIAKAAREAGILTVAVVTKPFAFEGKRRMQAAEQGIARLRECV
                     DTVIVIPNQNLFRVADAKTTFADAFSMADHVLYSGVGCITDLIVKEGLINLDFADVKS
                     VMRDMGRAMMGTGEASGEGRAKLAAEAAIANPLLDEASMIGAKGVLVSISGGMDMTLF
                     EVDEAATRIREEVDADADIIVGAIFDKSLEGKFRVSVVATGLRQEMSIAS"
     gene            5567..6820
                     /locus_tag="VGM46_06570"
     CDS             5567..6820
                     /locus_tag="VGM46_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006330125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit beta family protein"
                     /protein_id="HEY4192293.1"
                     /translation="MKYSIFSLARAALSGHRNWPRTWRDPTPKPRYDVVIIGGGGHGL
                     ATAYFLAEKYGIRNVAVLEKGWIGSGNAGRNTTIIRSNYMLPGNTGFYELSMKLWERM
                     EQDLNYNTMVSQRGIVNLYHSDAQRDAFARRGNVMRINGIDAELLDQAQIRKMMPFLN
                     FDNARFPIQGGLLQRRAGTARHDAVVWGYAHAGDRLGVDIIQNCEVTGFTRDQNGKVT
                     GVETSRGSIGADKVGMAVAGSSSRVAAMAGLRLPIESHVLQAFVSEAIKPLIPGVITF
                     GAGHFYVSQSDKGGLVFGGDIDGYNSYAQRGNMPTMEDVCEGGMALMPMIGRVRLLRQ
                     WGGIMDMSMDGTPIIDKTPVDGLYLNAGWCYGGFKATPASGLVFAHLLATDTPHDEAR
                     RFRLDRFQRGAMIDEKGVGAQPNLH"
     gene            6834..7139
                     /locus_tag="VGM46_06575"
     CDS             6834..7139
                     /locus_tag="VGM46_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HEY4192294.1"
                     /translation="MRIACPFCGERENGEFTYLGDATPKRPAIVAIDDAVDASAEAAA
                     FHDYVYLRDNIAGDMSEYWYHGGGCRSWLIVERNTLSHEILSVRAVPGAGAAKAGAK"
     gene            7139..>8405
                     /locus_tag="VGM46_06580"
     CDS             7139..>8405
                     /locus_tag="VGM46_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY4192295.1"
                     /translation="MAVQANRLAKGGLIDRNAPLDFTFDGKPMTGFAGDTLASALVAN
                     GVRLVGRSFKYHRPRGILTAGPEEPNALVELRSGARREANTKATTIELYDGLAAQSQN
                     RWPSLGFDVMSVNSLLSPIFVAGFYYKTFMWPAKLWEKLYEPAIRRAAGLGVSSGVAD
                     PDHYDKAWAHCDVLVVGAGPAGLAAALAAGRSGARVILAEEDPLPGGRLNSDGGEIDG
                     DDATVWLASALTELKSLPNVRIMTRTSVFGVYDGGTYAALERISDHIAAPPPHQVRQR
                     LWRIVARRSIVAAGAVERPIVFSGNDRPGVMMASAVRSYVERFAAAPGKRLALFTNND
                     DGWKTVETAMRAGISIAAVIDSRGTMPDRYRALAQSAGFSLHHGAVADVHGGAKGVTA
                     IEIALTGGGKATVAADVLAMSGGWNPSVGL"
BASE COUNT         1437 a         2849 c         2619 g         1500 t
ORIGIN      
        1 aaatcgccgt gatcggcgga gcgcaccttc tacaggaaag ttacggcgca aaatagtcgg
       61 aaagattgag gcgatattcg ccggcggacg aatgaacagg ctctaaaacg gctgaaattt
      121 ctgcattgga gccgttttcc gccgattcat ggatggaagg ccgaaatgtc tctcacgccg
      181 ggccggtgac ggcgggcggc ggcgcggcca tggcagccga acgcacatgc aatgttagac
      241 gctgaagccg acccgttttc gacagcgata attcttgact agttaactca actatttata
      301 gctgccccca gcagaccctg cacattggag aacctcatga agtccatcct atccgccctg
      361 gccggcgtcg ccgttctggc cagcctgtcc atctccccgg cattcgcgca ggaagacggc
      421 atcgtcgtct acaacgccca gcacgaaagc ctggccaagg aatgggcgga aggcttcacc
      481 aaggaaaccg gcatcaaggt gacgctgcgc aacggcggcg acagcgagtt ttccaaccag
      541 atcgtggccg aaggcgcatc ctcccccgcc gacgtgttcc tgacggaaaa ctcgcccgcc
      601 atggtgctgg tcgagaatgc cggcctgttc gcgccgctcg acgccgatac gctggcccag
      661 gttcccgaac agttccgccc ctcgaccggc aagtggaccg gcatcgccgc gcgcagcacc
      721 gtgttcgcct acaacaagac caagctcaag gccgaccagc tgccgaagtc gctgctcgac
      781 ctggccgatc cgagctggaa gggccgctgg gccgcctcgc cctcgggcgc cgatttccag
      841 gccatcgtca gcgccctgct cgaactgaag ggcgaggaag ccacctccgc ctggctgaag
      901 gcgatgaagg aaaacttcac cgcctataag ggcaacggca cggtgatgaa ggccgtcaat
      961 gccggcgaga tcgaaggcgg cgtgatctat cactattatt ggttcggcga tcaggccaag
     1021 accggcgaga acagcggcaa tgtcgaactg cactacttca agaaccagga tccgggcgcg
     1081 ttcgtctcga tctccggtgg cggcgtgctg gcttccagca agcatgccaa ggaagcgcag
     1141 gccttcgtca agtggattgc cggcaagggc ggccaggacg tgctgaagaa cggcacctcc
     1201 tatgaatacg ccatcggcaa tggcgcggat tccaacccca agctggtgcc gctggccgac
     1261 ctccaggccc ccaaggtcga gccttccaag ctgaattcga agaaggtcac cgacctgatg
     1321 acggcagccg gcttgcttta accgcgctac gtcctaaaaa ggcatcgaac cacgctcccg
     1381 cggacaagtc cgcgggagcc tttcgtttca aagatggcat ctgccaatga caacaagccg
     1441 gcccctggcc ccgtcgacca ccacattcgc cgcgtctttc gcctgatgca gccgtcgccc
     1501 cattatgcgg atgcagccgt gccgttcggc acggcgcggg gccgcttggc cggcaggctt
     1561 cccgtgtcat ggatcatgct ggccgcgctc acggtcaccc tgttcgcgct gctgccgctc
     1621 ggtttcgtcg tcgtcgtcgc catccagagc ggctgggata ccgtctacgc gctggtgttc
     1681 cgccctcgcg tcggcgaact tcttatcaac accgtcctgc tgaccgtcgt tgccgtgccg
     1741 atctgtgtcg ccctgtccat cgcgctggcc tggctgaccg agcgcagcga ccttcccgcc
     1801 gcgcgcctgt gggcatggct gtcggtcgcc ccgctcgccg tcccggcctt cgtccactcc
     1861 tatgcctggg tgacgctggt gcctggcctg cacggcctgt tcgccggcgt gctggtgtcg
     1921 gtcatcgcct attttccgtt cgtctatatg ccgatcgccg cttcgctgcg ccgtctcgac
     1981 cccgccttgg aagatgctgc cgcctcgctc ggccacccgc catggcgggt tttcctgcgc
     2041 gtcgtgctgc cgcaactgcg ccttgccatc tgcggcggct ctctgctggt cggcctgcat
     2101 ctgctggccg aatacggcct cttcgtcttc atccgcttcg atacgttcac cacggccatc
     2161 gtcgaccagt tccagtccac cttcaacgga ccggcagcca acatgctggc cggcgtgctg
     2221 gtcacctgtt gcctcgttct gctggccatc gaggtgctgg tgcgcggcga ggagcgttat
     2281 gcgcgcgtcg gctccggcgc ggcgcgccag cttcgcgtca cccggcttgg ctgggcgacc
     2341 gtcccctgcc tgcttttgac ggcgcttgtc gccgtgctgt cgctcggcgt gccgttcatg
     2401 accatcggcc gctggctcat cgccggcggc acggcggtct ggcaatggga cgagatcatg
     2461 cagacgcttg gccacacgct gttcctggcc gttgccggcg gcctgctggc caccattgcc
     2521 gccattccga tggcgtggct gtcgatccgt gcaccagggc cgctgcaacg cgtgctggaa
     2581 ggctccaact atgttgtcgg ctcgctgccc ggcgtcgtcg tcgcgctggc gctggtgacg
     2641 attaccgtgc gcgtggcgct gccgctctac cagacgcttt tcaccatcgt cgtcgcctat
     2701 gcgctgatgt tcctgccgcg cgcgctgatt tcgctgcgcg cctcgattgc gcaggccccg
     2761 atcgagctgg aacgcgccgc cggcagcctc gggcggcgac cgatcaacgc gctgtggtcg
     2821 acgaccattc gcctgtcggc gccgggtgcc gcggccggaa tggcgctggt ggcgctcggc
     2881 atcatgaacg agcttaccgc cacgcagatg cttgccccca acggcacccg cacgctggcc
     2941 ctggcgttct gggcctatag cagcgaaata gactatgcgg ctgcggcccc ctatgcgctg
     3001 atcatggtgg cggtgtcgct gccgctgacg tggctgctct acgtccaatc gaagcggatg
     3061 gccgggcgat gagctttctg gaattcaaca ccatacacaa gcgctacggg caggtggccg
     3121 cccttgccgg cgtcgacctg cgcgttgccg ccggcacccg cacggccatc gttggccctt
     3181 ccggctgcgg caagaccacc cttttgcggc tgatcgccgg ttttgaggcc ccggaccggg
     3241 gccgcatcgt gcttgacggc gaagtgctgg ccgaagatgg cgccgccgtg ccggcgcatc
     3301 tgcgcggcat cggcctcgtg gcgcaggatg gcgcgttgtt cccgcatttg accatcgccg
     3361 acaatatcgg cttcggcatg gagcggcgcg agacggggcg cgacgcccgc atcaccgagc
     3421 ttgcctacat tgtcgggctg gacaaggcga tactgaagcg ccgcccgcac gaactttccg
     3481 gcggccagca gcagcgcgtg gctctggccc gcgccatggc ccgtaaacca cggctgatgc
     3541 tgctagacga accgttttcg gcgctcgaca ccggcttgcg cgtttccatg cgcaaggccg
     3601 tcgccgaatt gctggagacg gcgggaatag ccaccatcct ggtcacccac gatcaggcgg
     3661 aagccctgtc tttcgccgat caggtcgtgg tgatgcgcga cggcgagttc tcgcaggtcg
     3721 gcaccccgcg tgagctttac ctgaaaccga aggacaggat ggtcgccgaa ttcctgggcg
     3781 acgccatcat cctgccggcc aatatcaggg gcggcttcgc ccaatgcacg cttgggcgca
     3841 tcgccgtcga caccaaggaa acgcgcgaca atgcccgcat catgctgcgg cccgaacagc
     3901 tgcttttgaa gcgcacctcg cgcgaaggca tgtcgggaac gccggacatg ctgttcggcg
     3961 aagtggtgga agtggagttc ggcggcccgg tctgcatgat cgcggtgcgg ctggtgaaca
     4021 gccccgaacc ccgcgacgca gccgccattg gcaatacgcc gcttatcttg cgcaagcccg
     4081 gcttggacgc gccggcggtc ggcgaaatcg tgcgcatcag cgccaccggc aaggcccatg
     4141 tatttccctg aaatctggcc tcgcggctga tattatggcc tgaaaagccg gttttccgcc
     4201 cgcgacggca tggctggaaa acacttcgcc cttgcgccgc gctccgccct ctgattcctt
     4261 gcggcaaagc cgtgccggcc cgattcgcct gttcgtgacc ggttgcatgg acatgattgc
     4321 cggcctggcg ccggtttctt cgaggggatt tccgttcatg aacaccaagc ccagaccgga
     4381 aattgcagag atgcggccca agatttcggt cattggcgtc ggcggtggcg gcggcaacgc
     4441 cgtcaacaac atgatctcca aaagccttgc aggcgctgaa ttcatcgtcg ccaacaccga
     4501 cgcgcaggca ctgaccatgt cgaaggcctc gcggctgatc cagcttggcg tgcatgtcac
     4561 cgagggactg ggcgcaggct cgctgcccga agtcggccag gcggcggcgg tcgaatccat
     4621 cgacgagatc atggatcatc tcaccggcac ccatatgtgt ttcgtcaccg ccggcatggg
     4681 cggcggcacc gggacgggtg ccgccccggt gatcgccaag gccgcgcgtg aggctggcat
     4741 cctcaccgtc gccgtcgtca ccaagccctt cgccttcgag ggcaagcggc ggatgcaggc
     4801 cgccgaacag ggcatcgccc ggctgcgcga atgcgtcgac acggtcatcg tcatccccaa
     4861 ccagaacctg tttcgggtag ccgatgccaa gaccaccttt gccgacgcct tttccatggc
     4921 cgaccatgtg ctttattccg gcgtcggctg cattaccgac ctgatcgtca aggaaggcct
     4981 gatcaacctc gacttcgccg atgtgaaatc ggtgatgcgc gacatgggcc gcgccatgat
     5041 gggcacaggc gaggccagcg gcgaaggacg cgccaagctg gccgccgagg cggcaatcgc
     5101 caatccgctg ctcgacgaag cctcgatgat cggcgccaag ggcgtgctgg tctcgatatc
     5161 gggcggcatg gacatgacgc tgttcgaggt cgacgaagcc gccacccgca tccgcgagga
     5221 ggtcgatgcc gatgccgata tcatcgtcgg cgccatcttc gacaagtcgc ttgaaggaaa
     5281 gttccgggtt tccgtggtcg ccaccggcct gcgccaggaa atgtccatcg cctcctgagg
     5341 caggttgccg ccgtatgacc ggaccttgcc gcggccattc gcaggccggc tgcatttcac
     5401 ctcttgcccg ccatggcgga tgacgaccgg ccctgcgtca tgtcgccata gcgacggcca
     5461 tgcaggaaat ccgaccccaa ccgccgctca atttacggaa tccgtgacgc cgatgcgata
     5521 acaagaagca attgcgttcg gcgatgccgc gcggcggaaa ataaagatga aatattcgat
     5581 cttctccctg gcccgcgccg ctctgtcagg gcaccgcaac tggccccgca cctggcgcga
     5641 cccgacgccc aagccgcgct acgacgtggt gatcatcggc ggcggcggcc atggcctcgc
     5701 caccgcctat ttccttgccg agaaatacgg catccgcaac gtcgccgtgc tggagaaggg
     5761 ctggatcggc tccggcaatg ccggccgcaa caccaccatc atccgctcca actacatgct
     5821 gcccggcaat accggcttct acgaattgtc gatgaagctg tgggagcgga tggagcagga
     5881 cctcaactac aacacgatgg tcagccagcg cggcatcgtc aatctctacc attcggacgc
     5941 ccagcgcgac gccttcgccc ggcgcggcaa cgtcatgcgc atcaacggca tcgatgccga
     6001 attgctggat caggcgcaaa tccgcaagat gatgcctttc ctcaatttcg acaatgcgcg
     6061 ctttcccatc cagggcggcc tgttgcagcg ccgcgccggc accgcccgcc acgatgcggt
     6121 ggtctggggc tatgcccatg ccggcgaccg tctgggcgtc gacatcatac agaattgcga
     6181 ggtcaccggc tttacccgcg accagaacgg caaggtcacc ggcgtcgaga cgagccgcgg
     6241 cagcatcggc gccgacaagg tcggcatggc ggtggccggc agttcctcgc gcgtcgccgc
     6301 catggccggc ttgcgcctgc cgatcgaaag ccatgtgctt caggctttcg tttcggaggc
     6361 catcaagccg ctcattcccg gcgtcatcac cttcggcgcg gggcatttct atgtcagcca
     6421 gtccgacaag ggcgggctgg tcttcggcgg cgacatcgac ggctacaatt cctacgccca
     6481 gcgcggcaac atgccgacca tggaagacgt ctgcgaaggc ggcatggcgc tgatgccgat
     6541 gatcgggcgc gtgcgcctgc tgcgccaatg gggcggcatc atggacatgt ccatggacgg
     6601 cacgccgatc atcgacaaga cgccggtcga cggtctctat ctcaacgccg gctggtgcta
     6661 tggcggcttt aaggcgacgc ccgcctcggg cctggtgttc gcgcatcttc tggcgaccga
     6721 cacgccgcac gatgaagcca ggcgcttccg cctcgaccgc ttccagcgcg gcgcgatgat
     6781 cgacgaaaag ggcgttggcg cccaaccgaa cttgcactga aggcggatca gcaatgcgta
     6841 ttgcgtgccc attttgcggc gagcgggaaa atggcgaatt cacctatctg ggcgacgcca
     6901 cgccgaaacg tccagccatc gtcgccatcg acgacgccgt cgacgcttcg gccgaagctg
     6961 ccgccttcca tgattatgtc tatctgcgcg acaacatcgc cggcgacatg agcgaatatt
     7021 ggtaccatgg cggcggctgc cgctcctggc tcatcgtcga gcgcaacacg ctgtcgcatg
     7081 aaatcttgtc cgtgcgcgcc gtgcccggcg caggcgcggc aaaagcgggt gcgaaataat
     7141 ggccgtgcaa gcaaaccgtc tcgccaaggg cgggctgatc gaccgcaacg ccccgctcga
     7201 tttcaccttc gacggcaagc cgatgaccgg ctttgccggc gatacgctgg cctcggcgct
     7261 ggtcgccaac ggcgtcaggc tggtcggccg ctcgttcaaa tatcaccgcc cgcgcggcat
     7321 cctcaccgcc gggccggaag agccgaacgc gctggtcgaa ctacgcagcg gcgcgcgccg
     7381 ggaagccaac accaaggcga caaccatcga gctttatgac ggtcttgccg cgcaaagcca
     7441 gaaccgctgg ccgtcgctcg gtttcgacgt catgtctgtc aactcgctgc tgtcgccgat
     7501 cttcgtcgcc ggcttctact acaagacctt catgtggccg gccaagctat gggaaaaact
     7561 ctacgagcct gccattcgcc gcgccgccgg ccttggcgtt tccagcggcg tcgccgaccc
     7621 ggaccactac gacaaggcat gggcgcattg cgacgtgctg gtggttggcg caggccccgc
     7681 cggccttgcc gccgcgctgg ccgccgggcg tagcggcgcg cgcgtgatcc tggccgagga
     7741 agacccgctg cccggcggcc ggttgaactc ggacggcggc gaaatcgacg gcgacgacgc
     7801 aaccgtctgg ctcgcctccg cgctgactga gctgaaatcg ctgcccaatg tccgcatcat
     7861 gacgcgcacc agcgtcttcg gcgtttatga cggcggaacc tatgccgcgc tggaacggat
     7921 cagcgatcac atcgctgcgc cgccgccgca tcaggtccgc cagcggctct ggcgcatcgt
     7981 cgccagacgc agcatcgtcg cggcgggcgc ggtcgaacgg ccgatcgtct tttccggcaa
     8041 cgaccgcccc ggcgtgatga tggcgtccgc cgtgcgcagc tatgtcgaac gcttcgcggc
     8101 ggcccccggc aagcggctgg ccttgttcac caacaatgac gatggctgga aaaccgtcga
     8161 aaccgccatg cgcgccggca taagcattgc ggcggtaatc gattcgcgcg gcacgatgcc
     8221 ggaccgctac cgcgcgctgg cgcaaagcgc cggcttttcc ctccatcatg gcgcggtcgc
     8281 cgatgttcac ggcggcgcaa agggcgtgac ggccatcgaa atcgcgctga ccggcggcgg
     8341 caaggcgacc gtcgccgccg atgtgcttgc catgtccggc ggctggaacc cctcggtcgg
     8401 cctgt
//
LOCUS       DASYCS010000204        14146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4686891,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000204 DASYCS010000000
VERSION     DASYCS010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 14146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14146
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4686891"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="VGM46_06585"
     CDS             complement(<1..783)
                     /locus_tag="VGM46_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEY4192296.1"
                     /translation="MLTISAAEVDRALTFPGLIETLREAFRHGAVQPVRHHHGVERPD
                     GAGTTLLLMPAWTDFNAAGTSGNGHIGVKIVTVSPDNNAIGKPAVMGLYLLLNGATGE
                     PQAMIDGQRLTQWRTACASALGASYLAREDASRLLVIGAGALSPFLAKAHSAARPIKQ
                     IHIWNRTLANAEKVAAGLRADGFNASAATDLDAELAWADIVSSATISNNPLVKGALLR
                     PGTHVDLVGGFTPDMRECDDDAITRARVYVDTRAGAAKEAGDI"
     gene            complement(819..1154)
                     /locus_tag="VGM46_06590"
     CDS             complement(819..1154)
                     /locus_tag="VGM46_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HEY4192297.1"
                     /translation="MFNRLLLIVVFAPLAIILIALAVANRELVSFTLDPFNPGNPALT
                     LTLPLFIYLFLALVVGMVVGSLATWFRQGRYRKLARQRGLEAESLRQAAIRRPPLAPD
                     ANALAKPAA"
     gene            complement(1212..1496)
                     /locus_tag="VGM46_06595"
     CDS             complement(1212..1496)
                     /locus_tag="VGM46_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006200394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEY4192298.1"
                     /translation="MIKSELVQIIAARSPHLFLRDVENIVGAIFDEITDALAEGNRVE
                     LRGFGAFSVKNRPARTGRNPRTGESVEVEEKWVPFFKTGKELRERLNGGK"
     gene            complement(1573..2529)
                     /gene="sppA"
                     /locus_tag="VGM46_06600"
     CDS             complement(1573..2529)
                     /gene="sppA"
                     /locus_tag="VGM46_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEY4192299.1"
                     /translation="MAPRADDMIDRRRLRRKLTFWRVITLAIAALGLIALGSWFYGDE
                     LSGAAVDHIAKVKIEGTITEDEELIERLEKIRKSPHVKGVILAVDSPGGTTAGGEMIY
                     EEVRKLAADKPVTAQVGTLAASAGYMIASAADHIVARQSSIVGSIGVLIQFPDVTGLM
                     DKLGIKLEEIKSSPLKAEPSPFNPTTDEERAMVRKLILDSYDWFVGIVAERRAMTRPQ
                     VLALADGSIFTGRQALANKLIDAVGGEDEAVAWLGTKGVDAKLKVVEWKNTQRSGGFL
                     FSRALVRTVGRTLGLPDSSGDMLHELGADRLFLDGLVSVWHP"
     gene            2776..3219
                     /locus_tag="VGM46_06605"
     CDS             2776..3219
                     /locus_tag="VGM46_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY4192300.1"
                     /translation="MPSTIRLHRVLTTNPEKVYRAFVEADALAKWLPPNGFVCTVHHL
                     EAKVGGTFEMSFRNFTTGASHSFGGEYVELNPGKGLRYTDKFDDPNLPGEMQVTVILK
                     KVSVGTEIDITQAGVPDVIPAEACYLGWQESLRNLAKLVEPEINQ"
     gene            3359..4507
                     /locus_tag="VGM46_06610"
     CDS             3359..4507
                     /locus_tag="VGM46_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ionic transporter"
                     /protein_id="HEY4192301.1"
                     /translation="MDLDRRTTADFGLPIGALLVAGAASLGGFALEHTALPARMAVIV
                     AVIGLLFATVFVVVRHADAVAHHLGEPYGTLVLTLSVTIIEASLIVSMMLHGANNPTL
                     ARESVFSTVMIVCAGVVGACLTLGGWRHHHQDIKRQGTSAFLAMLIAMTGFILVLPNY
                     TLAGDPGTFSTAQMAFVSTLALLLYGSFVLGQMNSHRGDFVDDMGDQPAPPRHGNPDQ
                     GAVRVASRAGEVRGVAISALLLVMGLAGIVLLAEQVAGGLEDSLAGFGVKQSDAIIGA
                     FIATLVLLPESVAAIKAALRNELQRSLNIALGSACATIGLTVPVVAAAGLLTGRTLTL
                     GLGQGDMVLLVVALGTSIVSFGTGRTTTMTGLVHLVVFFAYLLLIVVP"
     gene            4720..5427
                     /gene="lptC"
                     /locus_tag="VGM46_06615"
     CDS             4720..5427
                     /gene="lptC"
                     /locus_tag="VGM46_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEY4192302.1"
                     /translation="MLARPNETTGSDAGLPMADPAAPGPVRESAFGRAQRHSRRVRVF
                     KVVLPLIAGAIAIAFPVYSYLVTPPSVAVKADGSVFADGKLVMANPKLDGLTKENLPY
                     SLNALRAIQNAGREGIVELEGIDAKLPVSADNVAAVGASHGIYDRDKNTLNLDREITI
                     STTDGMVAKLKSAFLDMGKGTMTTSQPVDISREGSRITADTMSVRENGKVLVFEKRVR
                     LNIDPAAKNAAKADSGE"
     gene            5429..6037
                     /locus_tag="VGM46_06620"
     CDS             5429..6037
                     /locus_tag="VGM46_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HEY4192303.1"
                     /translation="MWATSSMRIAAAVSVTLLVGLGSAGAQSNSPSTMSGLKLSGDKP
                     IQIESDKLEVHQNENLAIFTGNVSVVQGPTLLKAGRMKVFYVKSDPAAAGKDKKAEAP
                     SKPAATGASPMSGGANIDHLEIDGKVYIKSDDQVATGDSGTFDMKSQVLVLSGKEVVL
                     SQGSNILVGCKLTVQMQTGLAQVDACGGRVMMSLTPQSQKAQ"
     gene            6043..6840
                     /gene="lptB"
                     /locus_tag="VGM46_06625"
     CDS             6043..6840
                     /gene="lptB"
                     /locus_tag="VGM46_06625"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007008121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEY4192304.1"
                     /translation="MVGLSRRPAEKLAAPAVTMGDATQFKGTLIAKGLSKSYKGRKVV
                     SDVTLGVRAGEAVGLLGPNGAGKTTCFYMVTGLVSADEGSIEIDGFDVTSMPMYRRAR
                     LGIGYLPQEASIFRGLTVEQNVRAVLEVVEKSRKERERSLDELLEEFHISHLRKAPSM
                     SLSGGERRRLEIARALATRPAYMLLDEPFAGIDPIAVADIQQLVRHLTARGIGVLITD
                     HNVRETLGLIDRAYIIHAGQVLTHGRAEEVVANADVRRLYLGEGFTL"
     gene            6995..8518
                     /gene="rpoN"
                     /locus_tag="VGM46_06630"
     CDS             6995..8518
                     /gene="rpoN"
                     /locus_tag="VGM46_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEY4192305.1"
                     /translation="MALAAKLQLKQSQSLVMTPQLMQSIRLLQLTHVELERFIDEEIE
                     RNPLLDRAEPQDDAASDQAQKAGEERDASARDDWYETDGVQSAEGMSDKLDASLENIF
                     PDDPGRSDAVGPDLAAQWKSASGSGGGASEGFDMEAMAAAAITLRDHVGEQIALSFHD
                     PALRLIAAELCDNLDDAGYLQADMAEIADRLGADQAKMAAALAVCQGFDPAGIFARDL
                     SECLALQLAVRDRLDPAMKALLANLDLLAKRDFQALKRLCGVDEEDILDMLAEIRLLD
                     PRPGMAFSTSGSETVVADVEVRAANDGSWAVELNPETLPRVLVDQVYFAHVSAHAQSQ
                     ADKDFLSECLQSANWLTRSLDQRAKTILKVTSEIVRRQDAFLLHGVRHLKPLNLRSVA
                     DAIGMHESTVSRVTANKYMLTPRGVFELRYFFTAAIASSEGGDAHSSAAVRDRIKQMI
                     DEEKPADVLSDDAIVDVLKADGIEIARRTVAKYREGMAIPSSVQRRREKRALASVGQ"
     gene            8783..9373
                     /gene="raiA"
                     /locus_tag="VGM46_06635"
     CDS             8783..9373
                     /gene="raiA"
                     /locus_tag="VGM46_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEY4192306.1"
                     /translation="MTLRISGKHMDIGDAFRTRITERVGEAIEKYFDRGFSGQVTIVK
                     AGSRYSADCMIRLDSGASLQAAGEAQDPTQAFDAAAERLEKRLRRYNRRLKSHYSNSA
                     GSNANSIDIAYTVMEPLADDDEEIPENFAPAIVAESSMALKTMSVASAVIELDTKDSP
                     VFVFRNAGNEHLNIVYRRPDGNIGWIDPSTTKLAQG"
     gene            9434..9898
                     /gene="ptsN"
                     /locus_tag="VGM46_06640"
     CDS             9434..9898
                     /gene="ptsN"
                     /locus_tag="VGM46_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HEY4192307.1"
                     /translation="MDLTDLIDVRAIMPALKANSKKQLLQLLSERAATISGIPEREIF
                     DTILQRERLGSTGVGNGIAIPHGKIVGVKHITGAFARLEAPVDFDALDDQPVDLVFLL
                     LAPEGAGADHLKALSRIARVLRDADIVAKIRGTHDAAAIHTLLSDTQASHAA"
     gene            complement(9966..10238)
                     /locus_tag="VGM46_06645"
     CDS             complement(9966..10238)
                     /locus_tag="VGM46_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002965410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1150 family protein"
                     /protein_id="HEY4192308.1"
                     /translation="MNRPEENTTNENFPMTNGEFAHLGEGSVAYLRKVSSDDLRGRFP
                     TLGEITPGLELWALFSANGQPILLSDARDRALAGAMENDLVTVAIH"
     gene            complement(10299..10724)
                     /locus_tag="VGM46_06650"
     CDS             complement(10299..10724)
                     /locus_tag="VGM46_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002965411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HEY4192309.1"
                     /translation="MSRMTPFSSPLLLGFDAMEKTLERLAKSGDGYPPYNIERLRGTE
                     GCVERLRITLAVAGFADEDLEITVEENQLVVRGRQNDDTEREFLHRGIAARQFQRCFV
                     LADGMRVMSADLKNGLLSIDLDRPEAERLVRKINISVKD"
     gene            10867..11808
                     /locus_tag="VGM46_06655"
     CDS             10867..11808
                     /locus_tag="VGM46_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006339190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEY4192310.1"
                     /translation="MPSQHRIIIDTDPGQDDAVAILLALGSAEIEVAGITVVAGNVPL
                     KLTENNARKVCELAGRRDIKIFSGAIRPLMRELVTAEEVHGQTGLNGPVLPEPTMPLQ
                     ERYAVDFIVETLMREPSGTITLCALGPLTNIALALVREPKIAARIKEIVLMGGGFFEG
                     GNITPTAEFNVYVDPHAADIVLKSGVSVVMMPLDVTHKALTNARRIVAIRGLGTKVGE
                     AVGDMLEFFERFDVEKYGSDGGPLHDPCVIAYLLKPELFKGRNCNVVVETASELTMGM
                     TVVDWWGVTKRPKNALFMRDIDHDGFFALLTERLARL"
     gene            complement(11805..12725)
                     /locus_tag="VGM46_06660"
     CDS             complement(11805..12725)
                     /locus_tag="VGM46_06660"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEY4192311.1"
                     /translation="MIIVIGSINLDMITAVERLPGPGETVRGSGFTTAAGGKGANQAL
                     AAARAEAAVRMVGSVGKDGFAAEALACLIEGKVDLTGVRESHASTGTAAILVDEAGEN
                     MIAVAPGANDTVLPGDLAKILLKRGDFVLLQHEIPLPTVDAALDAANAAGAVAVLNTA
                     PFRREAAGFLGKADYVVANETEFDFYAEALSLQGDDRPARMRDYAERTGRAVIVTLGG
                     DGVLAAAPGSFLEVPALKIVPVDTVGAGDTFCGYLGAALSAGLALDKALERAAAAGSL
                     ACLKPGAQPSIPHARDVDKALEDKALEDRT"
     gene            complement(12806..13699)
                     /locus_tag="VGM46_06665"
     CDS             complement(12806..13699)
                     /locus_tag="VGM46_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional helix-turn-helix domain-containing
                     protein/methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEY4192312.1"
                     /translation="MNAAMPLKPSATLEKDITPEGGDYEIVRRVIEKISLDYRDQPSL
                     EQLAEDVGETPTGLQKLFTRWAGLSPKAFLQAVTLDHARRLLDGGAPLLETSFEVGMS
                     GPSRLHDLFVTHEAMSPGDYKTRGAGLTIRYGYHISPFGIALIMVTDRGLAGLSFNNP
                     GEEQAAFADMSGRWPNAVYVEDMAATAPYAARIFDPARWRADQPLRVVMIGTDFQVRV
                     WEALLRIPLGKARTYSALAADIGAPDASRAVGAACGANPISFVVPCHRALAKSGALTG
                     YHWGLTRKRAILGWEAGKASA"
     gene            complement(13832..>14146)
                     /locus_tag="VGM46_06670"
     CDS             complement(13832..>14146)
                     /locus_tag="VGM46_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HEY4192313.1"
                     /translation="GLDVVGLYIAFRLNYRAARAREEVSVSRTALDIRKVAPSGKAEA
                     HRFNPFWTRFSVARHSEIGITEMAVEAQGRSVPIGGFLNPDDRESFADAFSRALATAK
                     KR"
BASE COUNT         2632 a         4257 c         4635 g         2622 t
ORIGIN      
        1 gatgtcgccg gcttccttgg cggccccggc gcgggtgtcg acatagacgc gggcgcgggt
       61 gatggcatcg tcgtcgcatt cgcgcatgtc gggcgtaaag ccgccgacga ggtcgacgtg
      121 cgtgcccggc cgcagcagag cgcccttgac cagtggattg ttcgagatcg tcgcggagga
      181 gacgatgtcg gcccaagcca gttcggcgtc gagatcggtt gccgcgctgg cattgaagcc
      241 atcggcgcgc aggccggccg ccaccttttc ggcgttagcc agggtgcggt tccagatatg
      301 gatttgcttg atcggccgcg cggccgaatg cgcctttgcc aggaaaggcg aaagcgcccc
      361 cgcgccgatc accagcaggc gcgaggcgtc ctcgcgggcc agataggaag cgcccagcgc
      421 cgaagcgcag gcggtgcgcc actgcgtcag gcgctggccg tcgatcatgg cctgcggttc
      481 gccggtcgcg ccgttcagca gcagatagag gcccatcacg gcgggcttgc cgatggcgtt
      541 gttgtctggc gatacggtga cgatcttgac gccgatatgg ccgttgcccg atgtgccggc
      601 ggcgttgaaa tcggtccagg ccggcatcaa aagcagcgtc gtgccggccc cgtcaggccg
      661 ctcgacgccg tggtggtggc gcaccggctg caccgcgcca tggcgaaacg cctcgcgcaa
      721 tgtttcgatc aagcccggaa aagtaagggc gcggtcgacc tcggcagccg agatggtcag
      781 catgaaaact ccgtgtggcg aatgaggttc gcggcgggtc aggccgccgg cttcgccagc
      841 gcgttggcgt cgggtgcaag cggtggccgc ctgatcgccg cctggcgcag gctttcggct
      901 tccaggccgc gctggcgcgc cagcttgcgg tagcggccct gcctgaacca tgtcgccagg
      961 ctgccgacga ccatgccaac gacaagcgcc agaaacagat agatgaacaa gggcaatgtc
     1021 agcgtcagcg ccggattgcc gggattgaac gggtcgagcg tgaaggacac cagttcgcgg
     1081 ttggccacgg ccagcgcgat caggatgatg gccagcggcg cgaaaaccac gatgagcagg
     1141 aggcgattga acatggcgtt ttgaacgtga ccttgaaaac cgacctgacg gatggccgtg
     1201 cgcccgacgg gttatttgcc gccgttcagc ctttcgcgca attccttgcc ggtcttgaag
     1261 aacggcaccc acttttcctc gacctcgacc gattcgccgg ttcgcgggtt gcgtccggtt
     1321 cgcgccgggc gattcttgac ggaaaacgcc ccgaaaccac gcaattcgac gcggtttccc
     1381 tcggcaagcg catcggtaat ctcatcgaag atggcgccga cgatgttttc gacgtcgcgc
     1441 aggaatagat gcggactacg tgcggcgatg atctgaacga gttcggattt gatcatgaag
     1501 cggccccgat ggagatatgc tgacaggaaa gagtgctgat tttattcggt ttcgttcgct
     1561 ttaagcgggc gttcaagggt gccagaccga gacgagaccg tcaaggaaca agcggtcggc
     1621 gcccagttcg tgcagcatgt cgccgctaga gtcgggcagg ccgagcgttc tgccgaccgt
     1681 gcgcaccagc gccctggaga acaggaagcc gccgctgcgc tgcgtattct tccactccac
     1741 caccttcagt ttggcatcga cgcccttggt gccgagccag gcgaccgcct cgtcttcgcc
     1801 gcccaccgcg tcgatcagtt tgttggccag cgcctgacgg ccggtgaaga tcgagccatc
     1861 ggcaagcgcc agcacctgcg gccgcgtcat ggcgcgccgt tcggcgacga tgccgacgaa
     1921 ccagtcatag ctgtcgagaa tgagtttgcg caccatggcg cgctcttcgt cggtggtcgg
     1981 gttgaacggc gagggttcag ccttgagcgg cgacgacttg atttcttcaa gcttgatgcc
     2041 gagcttatcc atcaggccgg tgacgtcggg aaactggatc agcacgccga tggagccgac
     2101 gatcgaggac tggcgcgcca cgatatggtc ggcggcgctg gcgatcatat atccggcgga
     2161 ggcggccagc gtgccgactt gcgccgtcac cggcttgtcg gccgccagct tgcggacttc
     2221 ctcatagatc atctcgccgc cggcggtggt gccgccggga gaatcgacgg cgagaatgac
     2281 gcccttgacg tggggagact tgcggatttt ctccagccgt tcgatcagtt cctcgtcttc
     2341 ggtgatggtg ccttcgatct tcaccttggc gatgtggtcg acggccgcgc cggacaattc
     2401 atcgccgtag aaccatgaac cgagcgcgat cagcccaagt gccgcgatag ccagcgtaat
     2461 tacccgccag aaagtcagct tgcgccgcaa ccggcggcgg tcgatcatgt cgtcggctct
     2521 tggtgccatc gtcggcctcg cattcgaaat tcagcgcaag ccgtcacttt tagaacatgc
     2581 gccagccgac cgccacccat ttcgagcggc cgcgcggctg agatcgcccg cgccaagcca
     2641 tcggtgatcg gcggccattt tcgaggcaaa agcttcgagg cgaaattttt ttgaggtaag
     2701 ctgtgcctcg tgtcggaccg gcagcgtcgc gttcgtcctt agggggtcaa caccttaagc
     2761 tggaaggata gaaaaatgcc gagcaccata cgcctgcacc gggtactgac gacgaacccg
     2821 gaaaaggtct accgcgcgtt tgtcgaggcg gatgcgctgg ccaaatggct tccgccgaac
     2881 ggcttcgtct gcaccgtgca tcatttggag gcaaaggtgg gcgggacgtt tgaaatgtct
     2941 ttccgcaatt tcaccaccgg cgccagccat tcgtttggcg gcgaatatgt cgaactcaat
     3001 ccgggcaagg gcctgcgcta caccgacaag ttcgacgacc ccaacctgcc cggcgaaatg
     3061 caggtgaccg tcatcctgaa aaaggtctcg gtcggcaccg agatcgacat cacgcaggct
     3121 ggcgtaccgg atgtcattcc ggcggaggcc tgttatctgg gctggcagga atcgctgcgg
     3181 aacctggcaa agctggtcga gcctgagatc aatcaataag gcgctcctgc gccgacattg
     3241 cgttaatcgc ggaatcaagc aaactggcgc gatgcacgtc cgcccagtcg gcttcgtccg
     3301 atccggcagg cgatgtttgg ctgtcgcctg gcgcgcatcg gggaaatatc ggcacaatat
     3361 ggaccttgac cgcaggacaa cagccgattt cggacttccc ataggcgcgc tgctcgtcgc
     3421 cggggcggcc tcgcttgggg gatttgcgct ggaacatacg gcgctgccgg cgagaatggc
     3481 ggtgatcgtc gccgtcatcg gcctgctttt cgcaaccgtc ttcgtggtcg tgcgccatgc
     3541 cgacgccgtc gcgcaccatc tgggcgaacc gtacggcacc ttggtgctga cgctgtcggt
     3601 cacgatcatc gaggcgtcgc tgatcgtatc gatgatgctg catggcgcca acaacccgac
     3661 gctggcgcgg gaatcggtgt tttccacggt gatgatcgtt tgcgccggcg tggtgggcgc
     3721 gtgccttacg cttggcggct ggcgtcatca ccatcaggat atcaagcggc agggcacgag
     3781 cgctttcctg gccatgctga tcgccatgac cggcttcatc ctcgttttgc cgaactatac
     3841 gcttgccggc gaccccggca cgttctcgac ggcgcagatg gccttcgtca gcacccttgc
     3901 gttgcttctt tatggcagct tcgttctcgg gcagatgaac agccatcgcg gcgatttcgt
     3961 cgacgacatg ggcgaccagc cggcaccgcc ccgccatggc aaccccgacc agggggccgt
     4021 ccgggtggct agccgggctg gggaggtccg gggcgtggcg atcagcgccc tgttgctggt
     4081 catgggcctt gccggcatcg ttcttctggc cgagcaggtg gcgggcgggc tggaggacag
     4141 cctggccggc tttggcgtga aacagtccga cgccatcatc ggcgcgttca tcgccacgct
     4201 tgtgctgctg ccggaatcgg tggcggctat caaggcggca ctgcgcaatg agttgcagcg
     4261 cagcctgaac atcgcgctcg gctccgcctg cgcgacaatc ggcctgaccg tgcccgtggt
     4321 cgccgccgcc ggcctgctta ccggccgcac gctcaccctc ggtctggggc agggcgacat
     4381 ggttctgttg gtcgtcgcct tgggaacgag catcgtcagc ttcggcaccg ggcggacgac
     4441 gacgatgacc ggcctggtgc atctggtggt gttttttgcc tatttgcttt tgatcgtagt
     4501 gccttaagcc cggtccggcc gccgacggga ttggcctgcg cttgaaggcg ataatggcgc
     4561 agcggtcatc acgctgctgt tttttttggc cgataccgtg tatcggtcac atttttcgcg
     4621 gttttcgctt ccttcgtgct tgcctgaagg ggcgggcatc ccgatttacg ggacgatcgt
     4681 attcaccggg ccgaggcaac gggtcagaga aagcaattga tgttggcgcg accgaacgaa
     4741 acaaccggca gcgatgccgg cctgccgatg gccgatcccg cagcgccagg cccggtgcgc
     4801 gagagcgcct ttgggcgcgc ccagcgccat tcgcgccggg ttcgcgtgtt caaagtggtg
     4861 ctgccgttga tcgcgggcgc gattgccatc gcctttccgg tctattccta tctggttacg
     4921 ccgccctcgg tggcggtgaa ggcggatggc agcgtctttg cggacggcaa gctggtgatg
     4981 gccaacccca agctcgacgg cttgaccaag gaaaaccttc cctattcgct gaatgcgctg
     5041 cgcgccatcc aaaatgccgg ccgggaaggc attgtcgagc ttgaggggat cgacgccaaa
     5101 ttgccggtct cggccgataa tgtggctgcc gtcggcgcat cgcacgggat ttacgaccgt
     5161 gacaagaata cgctcaatct cgacagggaa atcaccatat cgacgaccga tggaatggtg
     5221 gcgaagttaa aatcggcctt tctcgacatg ggcaaaggca ctatgacgac tagccagccg
     5281 gtcgatataa gccgtgaagg atcgcggatc acagcggata cgatgtcggt gcgggaaaac
     5341 ggcaaggttc tggttttcga aaaacgcgtg cgtctcaata ttgatcctgc cgcaaagaac
     5401 gcggccaagg cagacagcgg agaataagat gtgggccacg agttcgatgc ggattgccgc
     5461 ggcagtctct gtcacgctgt tggtggggct cggctctgcc ggggctcaat ccaattcgcc
     5521 cagcacgatg tcgggcctga agttgagcgg cgacaagccg atccagatcg aaagcgacaa
     5581 gcttgaggtt caccagaacg agaacctcgc catcttcaca ggcaatgtca gcgtcgtcca
     5641 gggaccgacc ctgctgaagg ccggccgcat gaaggtgttt tacgtcaaga gcgatccggc
     5701 cgccgccggc aaggacaaga aggccgaagc gccgagcaag cccgccgcga ccggcgcttc
     5761 gccgatgtcg ggcggcgcca atatcgacca tctggaaatc gacggaaaag tctacatcaa
     5821 gtccgacgac caggttgcga ccggcgacag cggcacgttc gacatgaaaa gccaggtgct
     5881 ggtgctgtcg ggcaaggaag tcgtcttgtc gcaagggtcc aacattctgg tcggctgcaa
     5941 actgaccgtc cagatgcaga ccggcctggc gcaggtggac gcctgcggcg ggcgcgtgat
     6001 gatgtcgctg acgccgcaat cgcagaaagc tcagtaacat ccatggtggg actttcgaga
     6061 cgaccggctg aaaagcttgc cgcgcctgcc gtaaccatgg gtgacgccac gcagttcaaa
     6121 ggcacgttga tcgccaaggg tctgtcgaaa agctacaagg gccgcaaggt cgtcagcgac
     6181 gttacgctgg gcgtgcgcgc cggcgaggcg gtcgggctgc tcgggccgaa cggcgccggc
     6241 aagaccacct gtttctacat ggtcaccggg cttgtctcgg ccgatgaggg gtccatcgag
     6301 atcgacggat tcgacgtaac ctcgatgccg atgtaccgcc gggcgcggct tggcatcggc
     6361 tatctgccgc aggaggcctc catttttcgc gggcttacag tcgagcagaa tgtgcgcgcc
     6421 gttctcgaag tcgtcgagaa aagccgcaag gaacgcgagc gcagcctgga tgagctgctc
     6481 gaggaattcc acatcagtca tctgcgcaag gcgccgtcga tgtcgctgtc gggcggggaa
     6541 cgccgacgcc tggagatcgc gcgcgcgctg gcgacccggc ccgcctacat gcttctggac
     6601 gagccttttg ccggtatcga tccgattgcg gttgccgaca tccagcagct tgtgcgccat
     6661 ttgacggctc gcggcatcgg tgtcctgatc accgaccaca atgtgcgcga aacgctcggc
     6721 ctgatcgacc gggcctatat catccatgcc ggccaggtgc tgacccacgg ccgggccgaa
     6781 gaagtggtgg ccaatgccga tgtgcggcgg ctttacctcg gcgaaggctt cacgctctaa
     6841 cgccgatagc cggtcgttct ccatgaccga cgatggtctg aacgagcttc ggcaggctgc
     6901 tttttggcag atgcggcttg acaagcaaaa gccgggccag ttcctatgcc tgaccggaaa
     6961 tttcatccgg ctgtcataat ggtgaacccg gaacatggcg ctcgccgcga aactacagct
     7021 gaaacagtcg cagtcgctgg tgatgacgcc gcagctgatg cagtcgatcc ggctgttgca
     7081 gctgacccat gtcgagctgg agcgcttcat cgatgaggag atcgagcgca atccgctgct
     7141 ggaccgcgcc gagccgcagg acgacgccgc gagcgatcag gcgcaaaaag ccggtgagga
     7201 acgcgatgct tcggcgcgcg acgactggta cgagaccgat ggcgtgcaaa gtgcggaggg
     7261 catgtcggac aagctcgatg cttcgcttga aaacatcttt cccgacgatc cgggcagaag
     7321 cgatgcggtt gggccggatc tcgccgctca gtggaaatcg gcatccggca gtggcggcgg
     7381 cgcgtcggag gggttcgaca tggaggcgat ggcggcggcc gccattaccc tgcgcgatca
     7441 tgtcggcgag cagatcgcgc tttcgtttca cgatccggcg ctgaggctta tcgccgccga
     7501 gctttgcgac aatctcgacg atgccggcta tttgcaggcc gacatggccg aaatcgccga
     7561 ccgacttgga gcggaccagg caaagatggc tgcggcgctg gcggtctgcc aggggttcga
     7621 ccccgccggc attttcgccc gcgacctcag cgaatgcctg gccttgcagc ttgcggtgcg
     7681 cgaccgcctc gacccggcga tgaaggcgct gctggccaat ctcgaccttc tggccaagcg
     7741 cgatttccag gcgctgaagc gcctgtgcgg cgtggacgag gaagatatat tggacatgct
     7801 ggccgagata aggttgctcg atccccgtcc gggcatggct ttttcgacca gcggcagcga
     7861 aacggtggtc gccgatgtcg aggtgcgggc ggccaatgac ggcagctggg cggtcgagct
     7921 gaaccctgaa acgctgcctc gcgtgctggt cgatcaggtc tatttcgccc atgtttccgc
     7981 ccatgcgcaa agccaggccg acaaggattt tctctccgaa tgcctgcaaa gcgccaactg
     8041 gctgacgcgc agcctcgacc agcgggcgaa aacgatcctc aaggtgacgt cggagatcgt
     8101 gcgcaggcag gatgcctttc tactccatgg cgtgcggcat ctgaagccgc tcaacctgcg
     8161 ctcggtggcc gacgcgatcg gcatgcacga atcgacagtc agccgggtca ccgccaacaa
     8221 atacatgctg accccgcgcg gggtattcga gctgcgctat ttcttcaccg cggcaatcgc
     8281 ttcctcggaa ggcggcgacg cgcattcgtc ggcagccgtg cgcgaccgca tcaagcagat
     8341 gatcgacgag gaaaagccgg cggatgtgtt gtccgacgac gccatcgtcg acgtcctgaa
     8401 ggcggacggc atcgaaatcg ccaggcgtac ggtggccaag taccgggaag gcatggcaat
     8461 tccctcctcg gtgcagaggc ggcgcgaaaa acgcgctttg gccagcgtcg gacagtgaat
     8521 cgggtcgatt ccgttggttt tccgccttat ttgacaagct gcggggaact ggctagaagc
     8581 ccgcccgcat gagatgggcg tgcggcccgc atgcgtgaga tcggccagga tgacggcaat
     8641 ggggcggtcc gtggggcggt cggggaacaa tctgaaagct cgggcgtaaa aacagcgcaa
     8701 gtgaagctgc gaagcttgtg cgcacggacc acgggtataa actccagaca gtgtgaagct
     8761 tgacaggaag gtcgaattcc agatgacgct gcgcatatcg ggaaagcata tggacattgg
     8821 tgacgcgttt cgcacgcgca tcacggaacg ggtcggcgag gcgatcgaga agtatttcga
     8881 tcgcggtttt tcgggacagg tcacgatcgt caaagcgggg tcgcgctatt cggccgattg
     8941 catgatccgg ctggattcgg gcgcttcgtt gcaggcggcc ggcgaagccc aggacccgac
     9001 gcaggccttc gatgcggcgg ccgaacgcct cgaaaagcgc ctgcgccgct ataatcgccg
     9061 cctgaagtcg cattattcga acagtgccgg cagcaatgcc aattccatcg acatcgccta
     9121 taccgtcatg gagccgctgg ccgacgacga cgaggagatt ccggagaatt tcgcgccggc
     9181 aatcgtcgcc gaatcgtcga tggcgcttaa aaccatgtcg gtggcttccg ccgtgatcga
     9241 gctggacacc aaggacagcc ccgtctttgt tttccgcaat gccggaaacg aacatctcaa
     9301 catcgtctat cgccggcccg atggaaatat cggctggatc gacccctcca cgaccaagct
     9361 cgcgcagggc taagagctgt ctgaacgccg gagcggggac cggcgggacg gtgaagcaca
     9421 aggattttca attatggatc tgaccgatct gatcgacgtg cgggcgatca tgccggcatt
     9481 gaaagccaac tcgaagaagc aattgctgca attgctgtcg gagagggcgg ccacgatttc
     9541 cggcattccg gaacgcgaga tattcgacac catcctgcaa cgcgagcggc tgggttcgac
     9601 cggcgtcggc aatggcatcg ccatcccgca tggcaagatc gtcggggtca agcatattac
     9661 gggcgctttc gcccggcttg aggctcccgt ggatttcgac gcgctggacg accagcctgt
     9721 cgacctggtg tttctgctac tggcgccgga aggtgctggc gcggaccacc tcaaggcgtt
     9781 gtcccgcatc gcgcgggtgc tgcgcgacgc cgatatcgtc gccaagatca ggggcacgca
     9841 tgacgccgcg gccattcata cgcttttgtc ggacacgcag gcctcccacg cggcctgagc
     9901 atgttttcca gagcatgaaa aaagggccgc tcgcgcggcc ctttttgtcg gtttctgcaa
     9961 ttggttcagt ggatggcgac ggtgacgagg tcgttttcca tggcgcccgc cagcgcgcgg
    10021 tcacgcgcgt ccgaaagcag gatcggctgg ccgttggcgg aaaaaagcgc ccacagttcg
    10081 aggccggggg ttatttcgcc aagcgtcggg aagcggccac gcaggtcgtc gctcgatacc
    10141 ttgcgcagat aggcgacgga gccttcgccg agatgggcga attcgccgtt cgtcatcggg
    10201 aaattctcat tggtggtgtt ttcttcgggt ctgttcattt cagcctcctt ttcgcggatg
    10261 ccaccggggc catcctgcat gagagccatc ggtaaaggtc agtctttcac cgatatattt
    10321 attttccgta ccagtctttc ggcctccggg cggtcgagat cgatcgacag cagcccgttt
    10381 ttcaaatccg ccgacatcac gcgcatgccg tcggccagta cgaaacagcg ttggaattga
    10441 cgcgcggcaa tgccgcgatg gaggaactcg cgctcggtat cgtcattttg acggccgcgc
    10501 acgaccaact gattctcctc gacggttatt tcgaggtctt cgtcggcgaa gcctgcgacc
    10561 gccagggtta tcctcaaccg ctcaacgcaa ccttcggttc cgcgaaggcg ctcgatattg
    10621 tagggcggat acccatcgcc ggatttggca aggcgctcca gcgtcttttc catggcatcg
    10681 aagccaagta gaagcgggct ggaaaaaggc gtcattcgtg acatgctggc ctgtgtcctc
    10741 tcaagagcga catagatgga aaaacccggt tggcgttttt cccgttggtg ctgatatggt
    10801 ccgtcgcgcg gccaagttca agcaccgaca gccactatcg gaacagccat tcgacaagga
    10861 atgaaaatgc ccagccaaca ccggatcatc atcgacaccg accccggcca ggacgatgcc
    10921 gtcgcgatat tgctggcgct gggcagcgcc gagatagaag tggccggcat tacggtggtg
    10981 gccggcaacg ttccgctgaa gctgaccgag aataatgcgc gcaaggtttg cgaactggcc
    11041 ggacgccggg atatcaagat attctcgggc gcgatccggc cgctgatgcg tgaactggtg
    11101 accgccgagg aggttcacgg ccagaccggc ctgaacgggc cggtgctgcc ggaaccgacg
    11161 atgccgttgc aggaacggta tgccgtcgat ttcatcgttg aaacgctgat gcgggagcca
    11221 agcggcacca tcaccctatg cgcgctcggg ccgttgacca acatcgcgct ggcgctggtg
    11281 cgggagccga aaatcgcggc gcggatcaag gaaatcgtgc tgatgggcgg cggcttcttc
    11341 gaaggcggca acatcacgcc gacggcggaa ttcaacgtct atgtcgatcc gcacgccgcc
    11401 gatatcgtgc tgaaatccgg cgtttcggtc gtcatgatgc cgctcgatgt cacccataag
    11461 gcgttgacca acgccaggcg catcgtggcg atccgcggtc tcggcaccaa ggtcggcgag
    11521 gcggttggcg acatgctgga atttttcgag cgcttcgacg tggaaaaata tggcagcgat
    11581 ggcgggccgc tgcacgatcc gtgcgtgatc gcctatctgc tcaagccgga attgttcaag
    11641 ggccgcaact gcaacgtggt ggtggaaacc gcctcggaac tgacgatggg catgacggtg
    11701 gtcgattggt ggggcgtgac caagcggccc aagaacgcgc ttttcatgcg cgacatcgac
    11761 catgatggct tcttcgcgct tttgaccgag cggctggcaa ggctctaggt ccggtcttcc
    11821 agcgccttat cttccaaggc cttgtccacg tccctggcgt gggggatgga tggctgcgcg
    11881 ccgggcttga ggcaggcaag ggagccggcg gcggcggcgc gttccagcgc cttgtcgagc
    11941 gccagcccgg cggagagagc cgcgcccaga tagccgcaaa acgtatcgcc ggcgccaacc
    12001 gtgtcgacag gcacgatctt aagggcgggc acttcgagga aactgccggg cgcggccgca
    12061 agcacgccgt cgccgcccag cgtaacgatg acggcgcggc ccgttctttc ggcatagtcg
    12121 cgcatgcggg caggccggtc gtctccctga agcgacagcg cttcggcgta gaaatcgaat
    12181 tccgtctcgt tggcgacgac atagtcggct ttgccgagga agccggcggc ttcgcggcga
    12241 aacggcgcgg tgttcagcac cgcgaccgcg ccggccgcat tggcggcatc gagcgcggca
    12301 tcgactgtcg gcagcgggat ttcgtgttgt aaaaggacga aatcaccgcg cttgagcagg
    12361 attttggcca gatcgccggg tagcaccgtg tcgttcgcgc ccggcgccac cgcgatcatg
    12421 ttctcaccgg cctcatcgac gaggatcgcc gccgtgccgg tgctggcgtg gctttcacga
    12481 acgccggtaa ggtcgacctt accctcgatc aggcaggcca gcgcctcggc agcgaagccg
    12541 tccttgccga ccgatcccac catgcgcacc gccgcctcgg cgcgcgcggc agccagcgcc
    12601 tggttggccc ccttgccgcc ggcggctgtg gtgaagcccg agccgcgcac cgtttcgccg
    12661 ggaccgggca ggcgctcgac cgccgttatc atgtcgaggt tgatcgagcc gatgacgatg
    12721 atcatgaaat ggagtgtctc ccggatttaa ccgggaaact agcccttccg aaagtctccc
    12781 gccagacggc gatcgcggaa aaccgtcagg cggaagcctt gccggcctcc cagccgagga
    12841 tggcgcgctt gcgggtcagg ccccaatggt aaccggtcag cgcgccggac ttggccagcg
    12901 cccggtggca gggaacgaca aaggagatcg ggttggctcc gcaggccgcg cccacggcgc
    12961 gcgaggcgtc gggcgcgccg atatcggcgg ccagcgccga ataggtgcgc gctttgccga
    13021 gcggtatccg cagcagcgct tcccacacgc ggacctggaa atcggtgccg atcatgacga
    13081 cgcgcaaggg ctggtcggcg cgccagcggg cgggatcgaa gatgcgggcg gcatagggag
    13141 cggtggccgc catgtcctcg acatagacgg cgttcggcca gcgccccgac atatcggcga
    13201 aagcggcctg ctcctcgccg gggttgttga aggaaaggcc agccaggcca cggtcggtga
    13261 ccatgatgag ggcgatgccg aacggcgaaa tgtgatagcc gtagcggatc gtcaggcctg
    13321 cgccacgggt cttgtagtcg ccgggcgaca tcgcctcatg ggtgacgaaa aggtcatgca
    13381 ggcggcttgg tcccgacatg ccgacctcga acgaggtttc cagcaatggc gcaccgccat
    13441 cgagcagcct gcgggcatgg tcgagagtga ccgcttgcag aaacgccttg ggcgacaggc
    13501 cggcccatcg ggtgaacagc ttttgcaggc cggtcggcgt ttccccgaca tcctcggcca
    13561 gttgctccag cgaaggctgg tcgcgatagt cgaggctgat cttttcgatg acgcggcgca
    13621 cgatctcgta atcgccgcct tcgggcgtga tgtctttttc gagggtcgcg gacggcttta
    13681 gcggcatcgc ggcattcata agcatctggg tgttcatggc aacgtctcct tggccatgaa
    13741 tatcgtcgct caggccggcg caaaccaccc gaaacttgcc ttgcaccggt gctggatggc
    13801 gtgaagccaa gcggcaggca ggcttgggcg cctatcgctt tttggccgtc gccagcgcgc
    13861 gggaaaaggc gtcggcaaaa ctttcgcggt cgtcgggatt gaggaagccg ccgatcggca
    13921 cgctgcgccc ctgcgcttcc accgccattt cggtgatgcc gatttcgctg tgccgggcaa
    13981 cggaaaagcg ggtccagaac ggattgaaac ggtgcgcttc ggccttgccg gaaggggcta
    14041 ccttgcggat gtcgagggcg gtgcgtgaca ccgacacttc ctcgcgggcg cgcgccgcgc
    14101 gataattaag gcgaaaggca atgtagaggc cgacgacgtc cagtcc
//
LOCUS       DASYCS010000205         3427 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2365460,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000205 DASYCS010000000
VERSION     DASYCS010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2365460"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /gene="ribD"
                     /locus_tag="VGM46_06675"
     CDS             complement(<1..646)
                     /gene="ribD"
                     /locus_tag="VGM46_06675"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEY4192314.1"
                     /translation="MKDPQNSAQEALDRRFMAAALRLSRRNAGRTATNPSVGTVIVRD
                     DGAGPMIVGTGVTAIGGRPHAETEALAEAGELARGATAYVTMEPCAHHGRTPPCANAL
                     VSAGIARVVGAASDPDARVSGKGYAILRAAGIEVVEKVLACEATEQMAGYLIRSIRKR
                     PEVVLKLALSSDGKIGRRGQGQVTITGEIARREVQMMRVEADAILIGVGTALEDD"
     gene            complement(651..1118)
                     /gene="nrdR"
                     /locus_tag="VGM46_06680"
     CDS             complement(651..1118)
                     /gene="nrdR"
                     /locus_tag="VGM46_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010915593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEY4192315.1"
                     /translation="MRCPYCQSEDTQVKDSRPAEDGSAIRRRRVCPDCGGRFTTFERV
                     QLRDLVVVKKSGRKVPFDRDKLLRSVEIAVRKRSVDPERIDRAVTGIVRQLESSGETE
                     VPTGEIGRLVMEALKSLDDVAYVRFASVYRNFREAKDFHDVLGELKGDDEDAV"
     gene            complement(1243..2556)
                     /gene="glyA"
                     /locus_tag="VGM46_06685"
     CDS             complement(1243..2556)
                     /gene="glyA"
                     /locus_tag="VGM46_06685"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019857608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEY4192316.1"
                     /translation="MASASAAQTFRDAFFETPLADVDPEIFGAIRNELGRQRHEIELI
                     ASENIVSRAVLEAQGSIMTNKYAEGYPGKRYYGGCQFVDVAEELAIERAKKLFGCNFA
                     NVQPNSGSQMNQAVFLALLQPGDTFMGLDLNSGGHLTHGSPVNMSGKWFKVVSYGVRQ
                     DDNLLDMDAIEKQAHETRPKLILAGGTAYSRIWDWKRFREIADSVGAYLMVDMAHIAG
                     LVAGGVHPSPLPHAHVVTTTTHKSLRGPRGGMILTNDEDIAKKMNSAVFPGLQGGPLM
                     HVIAAKAVAFGEALKPEFKLYAQNVADNAKALAESLKETGLDIVSGGTDNHLMLVDLR
                     PKNATGKRAEQALGRANITCNKNGIPFDPEKPFVTSGIRLGTPAGTTRGFGQAEFREI
                     GKLIAEVLDGLKAANSDEGNASVEAAVKQKVVALTDRFPLYPYLG"
     gene            2772..>3427
                     /locus_tag="VGM46_06690"
     CDS             2772..>3427
                     /locus_tag="VGM46_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY4192317.1"
                     /translation="MVHDFQADIDAIENIDAVPTILDVVCRTTGMGFAAVARVTESRW
                     ITCKVLDTINFGLVAGDELKVRSTICNEIRQSREAVVIDHVAEDEAYHDHPTPAMYGF
                     QSYISMPIIRSDGIFFGTLCAIDPRPARLKNPGTIGMFKLFAELIAAHLQAAEQLRAA
                     KTELAKERERSELRDQFIAVLGHDLRNPVASIDSGTKLLLRSPLEDKSRQIIKLMQGS
                     V"
BASE COUNT          636 a         1085 c         1055 g          651 t
ORIGIN      
        1 gatcgtcctc cagcgccgtt ccaacgccaa taagaatggc gtcggcctcc acccgcatca
       61 tctgcacctc acggcgcgcg atctcgccgg tgatggtgac ctgcccctgc ccgcgccggc
      121 cgatcttgcc atcgctcgaa agcgcaagtt tcagaacgac ttccggccgc ttcctgattg
      181 atcgaatcag atagccggcc atctgctccg tcgcttcaca cgccaaaacc ttctcgacca
      241 cctctatgcc ggcggcgcgc aatatggcgt aacccttgcc ggaaacgcgg gcgtccggat
      301 cgctcgccgc gccgacgaca cgggcaatgc cggcgctgac cagcgcgttg gcgcaaggcg
      361 gcgtgcggcc atgatgggcg catggctcca tcgtgacata ggcggttgcg ccgcgcgcca
      421 gttcgcccgc ctccgccaac gcctcggttt cggcatgcgg acgcccgcca atggcggtga
      481 cgccggtgcc gacgatcatc gggccggcgc cgtcgtcgcg cacgatgacc gtgccgaccg
      541 aaggattggt ggccgtgcgg ccggcattgc ggcgcgacag ccggagcgcg gccgccatga
      601 agcggcggtc cagggcctcc tgcgcgctgt tttgcggatc tttcatcggt ctagacggcg
      661 tcctcgtcgt cacccttcaa ttcgcccaga acatcgtgaa aatccttggc ttcacgaaaa
      721 ttccggtaga cggaggcgaa gcgcacatag gcaacgtcgt cgagcgactt cagcgcttcc
      781 atcaccagcc ggccgatctc gccggtcggc acttcggttt cgcccgagct ttcaagctgg
      841 cgtacgatgc cggtgacggc gcggtcgatg cgttcggggt cgacactgcg cttgcgcacg
      901 gcgatttcca ctgaacgcag cagcttatcg cggtcgaacg gcaccttgcg gcccgacttc
      961 ttgacgacga ccagatcacg cagctgcacg cgctcgaaag tggtgaagcg gccgccgcaa
     1021 tcgggacata cgcgacgcct gcggattgcc gagccatcct cggcggggcg cgaatccttc
     1081 acctgcgtgt cttcggactg gcaataaggg cagcgcatgg tcggtgtggc ctcgcgattc
     1141 tcgatctgcg gaaggattaa agcctttcag cgcgcatgca aacatgcctg cggcctcgct
     1201 accggcagat agcgaggccg cagggcaatt tccagccggc aatcagccga ggtaaggata
     1261 aagcgggaaa cgatcggtca atgcgacgac cttctgcttg accgcagcct ccaccgaggc
     1321 gttgccctcg tcggaattgg cagctttcaa accatccagc acttcggcaa tcagcttgcc
     1381 gatttcgcgg aattcggctt ggccaaagcc gcgcgtcgtg ccagccgggg tcccgaggcg
     1441 gatgccggag gtgacgaaag gcttttccgg atcgaaggga atgccgttct tgttgcaggt
     1501 gatgttggcg cggccaagcg cctgctcggc gcgtttgccg gtggcgttct tggggcgcaa
     1561 atccaccagc atcagatgat tgtcggtgcc gcccgaaacg atgtcgaggc cggtttcctt
     1621 gaggctttcg gccagcgcct tggcgttatc ggcaacattt tgcgcgtaaa gcttgaactc
     1681 gggcttcagc gcctcgccga acgcgacggc cttggcggcg atgacatgca tcagcgggcc
     1741 gccctggagg cctggaaaca ccgccgaatt catcttcttg gcaatgtcct cgtcattggt
     1801 gaggatcatg ccgccgcgcg ggccgcgcag cgacttatgc gtcgtggtgg tgacgacatg
     1861 ggcatgcggc agcggcgacg gatgcacgcc accggcaacg aggccggcaa tgtgggccat
     1921 gtcgaccatc aaataagcgc cgacgctgtc ggcgatctcg cggaaccgct tccagtccca
     1981 gatgcgcgaa taggcggtgc cgccggcgag aatcagcttc ggcctggtct cgtgcgcctg
     2041 cttctcgatg gcgtccatat ccaagaggtt atcgtcctgg cgcacgccat aggaaacgac
     2101 cttgaaccac ttgccgctca tgttgaccgg cgaaccgtgg gtcaggtgac cgcccgaatt
     2161 cagatcgaga cccatgaagg tatcgcccgg ctgcaacagc gccaggaaaa ccgcctggtt
     2221 catctggctg cccgaattcg gctggacgtt ggcgaaattg cagccgaaca gcttcttggc
     2281 gcgctcgatg gccaattcct cggccacgtc gacgaactgg cagccgccat aatagcgctt
     2341 gcccggatag ccctcggcat atttgttggt catgatcgac ccctgcgctt ccagcacggc
     2401 gcgcgagacg atgttctcgg aggcgatcag ctcgatctcg tggcgctggc ggccaagctc
     2461 gttgcggatg gcgccgaaga tttccggatc gacatcggca agcggggttt cgaaaaaggc
     2521 gtcgcgaaat gtctgggctg ccgatgcact ggccatgtct tggggttcct cgctgtccgg
     2581 atattgcgcc gccattagca cagttgttgc ggcgtcacca cgtttgccgc gcgaaatttg
     2641 ctggccgaaa tgcgccaaaa gcggcgaggc tggcctattg catagtttaa tgacgcgcac
     2701 gattctcgcc tgtataaatg cgcatgacgg gtcggtcctt aaccgaccgc ccagcctgga
     2761 gacaatactg cgtggttcat gattttcagg ccgacatcga cgcgattgaa aacatcgacg
     2821 cggtgccgac gatcctcgat gtggtctgcc ggactaccgg catggggttc gcggccgtcg
     2881 cgcgcgtgac ggaaagccgc tggatcacct gcaaggttct cgacaccatc aatttcgggc
     2941 tggttgcggg cgacgaactc aaggtccgtt cgacgatctg caatgaaatt cgccaaagcc
     3001 gcgaagccgt ggtcatcgac cacgttgccg aagacgaggc ctatcacgac cacccgacgc
     3061 ccgccatgta tggcttccaa agctatatct cgatgccgat cattcgcagc gacggcatat
     3121 tcttcggtac gctgtgcgca atcgaccccc ggccggcgcg gctgaaaaac cccggaacca
     3181 tcggcatgtt caagctgttc gccgaactta tcgcagcgca tctccaggca gccgagcagt
     3241 tgcgggcggc caagaccgaa ctggcgaagg agcgcgaacg ctcggaactg cgcgaccagt
     3301 tcatcgccgt gcttggccat gatctgcgca atccggtcgc gtcgatcgat tcgggcacca
     3361 agctgctttt gcgttcgccg ctcgaagaca agtcccggca aatcatcaag ctgatgcagg
     3421 gcagcgt
//
LOCUS       DASYCS010000206         1766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2512020,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000206 DASYCS010000000
VERSION     DASYCS010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2512020"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /locus_tag="VGM46_06695"
     CDS             complement(<1..999)
                     /locus_tag="VGM46_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEY4192318.1"
                     /translation="MSLIPKIGLPKGRATESIVDGVADGYEAFALVQAAQEIAPDRPV
                     LFVARDGQRLPSIVEALSFAAPGLPVLELPAWDCLPYDRVSPGSDAAARRLDALTAMI
                     ALARKPHRAVILTTANALLQRIPPAELIEAQNFHARPGNQIDMKTLVSRLETSGFDRV
                     PTVRSIGEFAVRGGILDLFAPGWPEALRLDFFGDTLESIRTFDAASQRTTGQRKSMSL
                     QAMSEVALTPETISRFRRAYIEAFGAPSRDDGLYAAVSEGRRFAGMEHWLPLFYERLD
                     TVFDYLPDTPVVFDHLAHEALAERHTLILDHYEARRKQADGALKDAVPYKPVSPDLL"
     gene            complement(1010..1297)
                     /locus_tag="VGM46_06700"
     CDS             complement(1010..1297)
                     /locus_tag="VGM46_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019858376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEY4192319.1"
                     /translation="MTGTKRSSEGLEPRRAKLLFRSWHRGMREMDLILGQFADAEIAA
                     LTDAEIDQYEQLLDIPDTEFLPWVTGERPVPTGLDSPLLQKILASRRSMTF"
     gene            1521..>1766
                     /locus_tag="VGM46_06705"
     CDS             1521..>1766
                     /locus_tag="VGM46_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEY4192320.1"
                     /translation="MRPFLLDPLFVPITSLEGVGPKVAGMIEKIVPADLGDRAARAGD
                     LLFVLPNTVIDRRNRPGIAYAAQGAIVTLELTIDRHQP"
BASE COUNT          322 a          561 c          578 g          305 t
ORIGIN      
        1 gagaagatcg ggcgacaccg gcttataggg cacggcatcc ttcagcgcgc cgtccgcctg
       61 cttcctgcgc gcctcgtaat gatcgaggat cagcgtatgg cgctcggcca gcgcctcatg
      121 cgccagatgg tcgaacacca ctggcgtatc cggcaggtag tcgaacacgg tgtcgagccg
      181 ctcgtagaac agcggcagcc agtgctccat gccggcgaaa cggcgacctt cactgaccgc
      241 ggcgtaaagc ccgtcatcgc gcgatggcgc gccgaaggcc tcgatgtagg cgcggcggaa
      301 gcggctgatg gtttccggcg tgagagccac ttcgctcatc gcctgcaacg acatcgactt
      361 gcgctggccg gtggtgcgct ggctggcggc atcgaaggtg cggatggatt ccagcgtgtc
      421 gccgaagaaa tcgaggcgca gcgcttcggg ccaaccgggc gcaaagaggt cgagaatgcc
      481 gccgcgcacg gcgaactcgc cgatggagcg cactgtcggc acacggtcga agccggaggt
      541 ttcaagccgc gacaccaggg ttttcatgtc gatctggttg cccggcctgg catggaaatt
      601 ctgcgcctcg atcagttccg caggcggaat gcgttgcagc agcgcattgg cggtggtgag
      661 gatgacggcg cggtgcggct tccttgccag ggcaatcatc gcggtcagcg cgtcgaggcg
      721 gcgcgcggcg gcatcggaac cgggcgaaac gcggtcgtaa ggcaggcaat cccaggcagg
      781 caattccaga accggcaggc cgggtgcggc aaaggaaagc gcttcgacga tggatggcag
      841 gcgctggccg tcgcgcgcca cgaacagcac cggccggtcc ggcgcgattt cctgcgccgc
      901 ctgcaccagc gcgaaggcct catagccgtc ggcgacgcca tcgacgatag attcggtggc
      961 acggcccttg ggcaggccga ttttagggat caggctcatc ggttcacgtt cagaatgtca
     1021 ttgagcggcg cgaggccagg attttttgca gcaacgggga atcaaggcct gtcggcaccg
     1081 ggcgttcgcc ggttacccaa ggcagaaatt cagtatcggg aatgtccaga agctgctcat
     1141 attggtcgat ttcggcgtca gtcaaggccg cgatctcggc atcggcgaac tgtccgagga
     1201 tcaaatccat ctcccgcatg ccgcgatgcc aggaacgaaa aagaagcttg gcgcgccgag
     1261 gctccagccc ctcgctcgat cgtttcgttc cggtcatggc aatgcgtcct gtctcagtgg
     1321 ggcgcatata gcgtggatag aggccggtgt cagcctttgc aaggcgcgtg gcctcgccta
     1381 gtttgggcaa tgtcttccat tgcagttcgg ctcacacccc cgcccccaat tcctcccgac
     1441 aagagggaag gagttgccaa cgccccggct acccggtccc cttcgtgggg ctggggtaac
     1501 ccacgggcga aaatctagcc atgcgcccct ttctcctcga ccctcttttt gtgcccatca
     1561 cctcgctcga aggcgtcggg ccgaaggttg ccggcatgat cgagaaaatc gtgccggccg
     1621 acctcggcga ccgggccgcg cgggccggcg atcttctgtt cgtgctgccc aacaccgtca
     1681 tcgaccggcg caaccggccg ggcatcgcct atgccgcgca aggcgccatc gtgacgctcg
     1741 aactcaccat cgaccgccac cagccg
//
LOCUS       DASYCS010000207         8924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3673601,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000207 DASYCS010000000
VERSION     DASYCS010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 8924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8924
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3673601"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1346)
                     /locus_tag="VGM46_06710"
     CDS             complement(<1..1346)
                     /locus_tag="VGM46_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019996791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY4192321.1"
                     /translation="MQQYQNADNLSFTASSPESATQFSAFTSRYMGFRRDTGATLKAL
                     VETDPDMPMAHCAKGYLAKLIGSSNHSARAKGISDGMAGLMDRTGANQRERSHAQALA
                     RWCAGDLDAATGIWEGILLDHPQDGLALRLAHFTHFYSGDPRRMRDSMARILPLWSPE
                     HRDYGFLLGMYAFGLEENGEYAAAEKYGRMAVERNPADAWSVHSVAHVLEMTERMEEG
                     IQWVTGLENSWSSVNNFRFHLYWHRCLYHLERGEFDTVLALYDKQIVSDIESDFYLDI
                     CNASSLLWRLEMFGIDVGSRWENLVGVAKRHVDDTDLIFVSLHYLMALVAGGDAVAAD
                     RMISNIRQWSMLDGTQAQVCAESGLALADGLKLARAGRYTEALAVMEPVRYRLDRIGG
                     SRAQRDVFHMILLDTAKSSRDSLRARALFAERLAHKAHSSWSWKGYGDILATLGQD"
     gene            complement(1357..2574)
                     /gene="acdA"
                     /locus_tag="VGM46_06715"
     CDS             complement(1357..2574)
                     /gene="acdA"
                     /locus_tag="VGM46_06715"
                     /EC_number="3.13.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019863718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-sulfinopropanoyl-CoA desulfinase"
                     /protein_id="HEY4192322.1"
                     /translation="MSAATKITSDLMAKARDLAEREFRPLAAEVDRTEAYPWESVAKL
                     AKAGFMGMTIPKEYGGQGLSYTDAVTVVEEMGRCCGTMSRITVEANMGALGAIMHYGS
                     DKQKRLSSELVLSGDKPAICITEPDAGSAATEMTTFAVKKGDKYIINGKKHWITGGGV
                     SKLHLVFARLMENGRSQGIAGFIAVRDETPGLKIGKREPAMGLRGIPETEIIFDDMEV
                     AEDMIVVPPEGVKRGFAGLMRAYNAQRVGAGTVALGIAQGAYEHAVDYALERQQFGRP
                     IAEFQGLQWMIADMSISLAAARALLYAAAGTVVDGFPDIRAAAQAKILAAETAQSVTS
                     ASLQIHGAMGYSRNLPLERMMRDARMFSIAGGTAQILRTQVAGSILGIKTPQTRDGYA
                     TRTEQPALGAAAE"
     gene            complement(2571..3833)
                     /locus_tag="VGM46_06720"
     CDS             complement(2571..3833)
                     /locus_tag="VGM46_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEY4192323.1"
                     /translation="MTTSTISAPEVTSAAANGPLEGLRILDLSRILAGPTCTQLLGDM
                     GADVLKIERPGEGDDTRSWGPPFVSDRDGVATRESAYYLCANRNKRSMALDIADPADA
                     AKIRALVRSCDIVIENFKVGGLKKYGLDYESLAAEKPGLVYCSITGFGQTGPHAAKPG
                     YDLLAQAYGGIMSLTGDPDGEPMKVGVGVADVVCGLYASNAILAALRHRDRTGEGQHI
                     DIALVDAQVAWLVNAGTNYLLSGRLPKRLGNQHPNIVPYQVFEVADGHVVVASGNDRQ
                     YRSFCDIIARPDLAAEPRFRTNDARIENRDALIELIAVELKAFTKQALVEAMEAAGVP
                     GGPINTLADVFSSDQVESRGMKISVPYPGSQTGEVQLIGNPIKFSRTPVTYRRAPPTC
                     GQHTLEILAEFGLLDEATQSAVERGRHS"
     gene            4596..5462
                     /locus_tag="VGM46_06725"
     CDS             4596..5462
                     /locus_tag="VGM46_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY4192324.1"
                     /translation="MKLRPGALHALRIPAVIAALLAFSHLPAAQAADMIGNCEVTGVK
                     GSMPFTPVNAGVLTVEVNLPSNPGWYNGDTPDQIKDGYEYCLAANIAHRAGLDGVKIV
                     NVAWDALVTGHTKDFDIALSQISITEERKKVVDFSTPYFNSDFGVLVKKGTTVDSTSI
                     KKMRFAVQQSTTGADFVTNKVQPAEVKVFPDAPGMVTALMAGQVEAAGNDTAVMLGWA
                     TRSQGKLEVVGQYTTGEAYGGLYPKASPNAAKINEIIEALIKDNTVKSLSTKYLGPVL
                     GADPTSVPYIKP"
     gene            5598..6635
                     /locus_tag="VGM46_06730"
     CDS             5598..6635
                     /locus_tag="VGM46_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY4192325.1"
                     /translation="MVSALLVTVLVIAATFRTALDVRAAAATLGVSGLVVDAILVVGC
                     LTVLSLLRPALKSMRQARAVSSALASNNVIDARVAAAASRDSAYYTLGSAVAVVAALL
                     LIQFIIANNLAVGRTFFLLPLIWSTIWLVIKAFWVNIYIFVVAEILVLIWGLIVAVAR
                     LMPGDAGRPVRMIATIYTDMFRGLPAIITIYLVGFGLSLTDLPWIKDLSQETFAIIAL
                     TLTHGAYTAEVYRAGIQSIHGSQVAAARSLGLSYFQTLRYVIVPQAVRRIVPPLLNGF
                     IGLQKDTALVNVIGAVDAFNQAKILTSNYFNLSPVTGVALLFILVTIPQARFVDKLIE
                     HDQRRTRAGGN"
     gene            6648..7391
                     /locus_tag="VGM46_06735"
     CDS             6648..7391
                     /locus_tag="VGM46_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEY4192326.1"
                     /translation="MAMIEIRDIHKHYGAYKVFDGLSLDVEEHQVVCLVGASGCGKST
                     LLRAMNGLEQIQSGEIRINADRITGPGVDVNALRRDVGIVFQSYNLFPHMTVLQNVTL
                     APIKVLGEDRHEAEARAISFLKRIGLEHKANEYPDRLSGGQQQRVAIVRAMAMGPMVL
                     LLDEITSALDPELVSEVLNIVRDLAAEGMTMLLATHEMAFAREVASKVCFLADGKVHE
                     EGPPEEIFGNPKQERTRTFLQRIIEAGRL"
     gene            7517..8749
                     /locus_tag="VGM46_06740"
     CDS             7517..8749
                     /locus_tag="VGM46_06740"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013844934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEY4192327.1"
                     /translation="MTAVAEIDTQNFQPASRIASVEVSEILRIGARAQALKKAGRPII
                     VLGAGEPDFDTPDHVKQAASDAIARGETKYTALDGTVALKNAIQRKFSRENAVEYALD
                     EITVGAGAKQILFNAMMATLNEGDEVIIPTPYWTSYSDIVRIAQGVPVFVTCDADAGF
                     RMTAKQLEAAITAKTRWVMLNSPSNPSGAAYSAADYRPLLDVLLKHPHVHLLVDDMYE
                     HIVYDGFEFVTPAALEPRLKNRILTVNGVSKAYAMTGWRIGYAGGPKALIEAMAVVQS
                     QATSCPSSVSQAAAVEALNGTQDFVGERCKSFQDRRDLVVAGLNAIDGIDCRVPDGAF
                     YTFASCAGLLGRKRPNGAIIATDADFCDYLLTEADVAVVPGSAFGLSPYFRISYATSS
                     DQLKEALQRMKQACERLA"
BASE COUNT         1647 a         2772 c         2753 g         1752 t
ORIGIN      
        1 tcgtcctggc cgagcgttgc aaggatgtcg ccatagcctt tccacgacca gctcgaatgg
       61 gccttgtggg cgaggcgttc ggcaaacagc gcgcgagcgc gcagcgaatc ccggctggac
      121 ttggccgtat cgagcaggat catgtgaaag acgtcccgct gcgcccggct gccgccgatg
      181 cggtcgagcc ggtagcgcac cggttccatc accgcgagcg cttcggtata gcggccggcg
      241 cgcgcgagct tgaggccgtc ggccagcgcc aggccggatt ccgcgcacac ctgcgcctgc
      301 gttccgtcaa gcatcgacca ttgccggata ttcgagatca tccggtcggc cgccacggca
      361 tcgccgccgg cgaccagcgc catcaggtaa tgcagcgata cgaagatgag atcggtgtcg
      421 tccacatgcc gcttggcaac acccaccagg ttttcccagc gactgccgac atcgatgccg
      481 aacatttcca gccgccacaa aagcgaggac gcattgcaga tgtcgaggta gaaatcggac
      541 tcgatatcgg agacgatctg cttgtcgtac agcgccagca cggtgtcgaa ttcgccgcgc
      601 tccagatggt acaggcatcg gtgccaatag aggtggaacc ggaaattgtt gacggatgac
      661 cagctgtttt ccagcccggt gacccactgg atgccttcct ccatgcgctc ggtcatttcc
      721 agtacgtgag cgacggaatg gacggaccag gcatccgccg ggttccgctc caccgccatc
      781 cggccatatt tctcggcggc ggcatattcg ccgttttctt cgaggccgaa ggcgtacatg
      841 ccaagcagga agccgtaatc gcggtgctcc ggcgaccaga gcggcaggat gcgcgccatc
      901 gaatcacgca tgcggcgcgg gtcgccggag tagaaatgcg tgaaatgggc cagccgcagc
      961 gccagtccgt cctgcgggtg gtcgagcagg atgccttccc atatgccggt cgccgcgtcg
     1021 aggtcgccgg cgcaccatct ggcaagcgct tgcgcatgcg agcgctcgcg ctggtttgcg
     1081 ccggtgcggt ccatcagccc ggccatccca tcggaaatgc ccttggcgcg cgccgaatgg
     1141 ttggaactgc cgatcagctt ggccagatag cccttggcgc agtgcgccat cggcatgtcc
     1201 gggtcggttt cgaccaaggc tttcagcgtg gcgccggtat cgcggcgaaa gcccatatag
     1261 cgcgaggtga aagcgctgaa ctgggttgcg ctttcggggg aagacgccgt gaaggaaagg
     1321 ttgtccgcgt tttgatattg ctgcatggcg atctcctcat tcggcagcag cgcccagcgc
     1381 cggctgctcg gttcgcgttg cgtagccgtc acgggtctgt ggcgtcttga tgccgaggat
     1441 cgaacccgcc acctgcgtgc gcaggatctg ggccgtgccg ccggcaatcg aaaacatgcg
     1501 cgcatcgcgc atcatgcgct caagcggcag gttgcgtgaa tagcccatgg cgccgtggat
     1561 ctggagcgag gcgctggtaa ccgactgcgc ggtctcggcg gcaaggattt tggcttgcgc
     1621 cgccgcgcga atgtcgggaa agccgtcgac gaccgtgccg gcggccgcgt aaagaagagc
     1681 gcgcgccgcc gcaagcgaga tcgacatgtc ggcgatcatc cactgaagcc cctgaaattc
     1741 ggcgatcggg cggccgaatt gctggcgttc gagcgcgtaa tcgacggcat gctcgtaggc
     1801 gccctgcgct atgccgagcg ccaccgtgcc ggcgccgacc cgctgggcgt tataggcgcg
     1861 catcaggccg gcaaaaccgc gcttcacgcc ttccggcggg acgacgatca tgtcctcggc
     1921 gacttccatg tcgtcgaaga tgatttcggt ttccggaatg ccgcgcaacc ccatcgccgg
     1981 ttcgcgcttg ccgatcttga ggccgggcgt ctcgtcgcgc accgcgatga agccggcaat
     2041 gccctgcgag cggccgtttt ccatgaggcg ggcaaagacg aggtgcagtt tcgatacgcc
     2101 gccgccggtg atccagtgct tcttgccgtt gatgatgtat ttgtcgccct ttttcacggc
     2161 gaaagtggtc atctcggtgg cggcgctgcc cgcgtcaggt tcggtaatgc agatggcggg
     2221 cttgtcgccg ctcaaaacca gctcgctgga gaggcgcttc tgcttgtcgg aaccgtagtg
     2281 catgatcgcg ccgagcgcgc ccatattggc ctcgaccgtg atgcggctca tcgtgccgca
     2341 gcagcgtccc atttcctcga ccacggtgac cgcatcggta taggacaagc cctggccgcc
     2401 atattccttg gggatggtca tgcccatgaa gccggccttg gcaagcttgg ccacgctttc
     2461 ccatggatag gcctcggtgc ggtccacctc ggcggccagc gggcggaatt cccgctccgc
     2521 caggtcgcgg gccttggcca tcaggtcgga ggtgattttg gttgcagcgc tcatgaatgc
     2581 cttcctctct ctacggcgga ttgcgtcgcc tcgtccagca ggccgaattc cgccaatatt
     2641 tccagcgtgt gctgcccgca ggtgggcggc gcacggcgat aggtgacggg ggtgcggctg
     2701 aacttgatcg gattgccgat cagttgcacc tcgcccgtct gcgatcccgg atagggaacg
     2761 gatattttca tgccccggct ttcaacctgg tcggacgaga acacgtcggc cagcgtattg
     2821 atggggccgc ctggaacgcc agccgcttcc atcgcctcga ccagcgcctg cttggtgaag
     2881 gccttgagtt ccaccgcgat caattcgatc agcgcgtcgc ggttttcgat gcgcgcgtcg
     2941 ttggtgcgaa aacgtggctc ggccgccaaa tccgggcgcg cgatgatgtc gcaaaagctg
     3001 cgatattgcc ggtcgttgcc ggaggccacc acgacatggc catcggcgac ttcgaaaacc
     3061 tggtagggca cgatgttggg atgctggttt cccagccgct tcggcaggcg gccagacagc
     3121 aggtagttgg tgccggcatt gacgagccat gccacctgcg cgtccaccag cgcgatgtcg
     3181 atatgctggc cttcgccggt gcggtcgcgg tggcgcaggg ccgccagaat ggcattgctg
     3241 gcataaagcc cgcacacgac atcggcgacg ccgacgccaa ccttcatcgg ctcgccgtcg
     3301 gggtcgccgg tcaggctcat gatgccgcca taggcctgcg caagcaggtc gtaacccggc
     3361 ttcgcggcat gcggcccggt ctggccgaac ccggtgatcg agcagtagac caggcccggt
     3421 ttctcggccg ccaggctttc atagtcgagg ccgtatttct tgagcccgcc caccttgaag
     3481 ttctcgatga cgatatcgca actgcgcacc aatgcccgga ttttcgcggc atccgccggg
     3541 tcggcgatat cgagcgccat cgaccgcttg ttgcggttgg cgcacagata ataggcgctc
     3601 tcgcgggtcg ccaccccgtc gcggtcgctg acgaagggcg gtccccagct tctggtgtcg
     3661 tcgccctcgc cggggcgctc gatcttcagc acgtccgcgc ccatgtcgcc cagcaactgg
     3721 gtgcaggtag gcccggccag aatgcgcgac aagtccagga tgcgcagccc ttcgagaggg
     3781 ccgtttgccg cggcgctggt tacttcaggg gcggaaatcg tcgatgtcgt catgatgatt
     3841 tcctaaataa ttgacataat tgatgcaagt ttaattatgt ggataataac atgacggaaa
     3901 ttgatgctat gtgacaagct gcgcgcgcct cttcatagaa gctgggcgct ttgcactcag
     3961 cggttaggcg ctggcgccat attttccgcg ccgcaacttt gcggctgcaa acgacagcac
     4021 tgggcgcttc cgcaccggta tccatcggcc gccacccgaa gatggcgtcg agtggacatt
     4081 ccgccgggac cgcgccccat gtcccgcgtt cccatgccac cgcattccat gcaattgacc
     4141 ggcccggccc atagacggca acggatcgtc aggcgcgggc gtcgttgtct cttgacaagc
     4201 tcatatgttc cggcattaat attcagaatg ccgttctata atgtagaacg tcattgggga
     4261 gtgagaacga atgaacgaac cattggcgcg gtgcgctgcg gctgtatctc cgcatgctgc
     4321 gttggaatca agccagagca gagggcaggg cggttttatg tcgccacgcg catttccgtt
     4381 ggcgtcaggc cgctggtatc gccacctctg gtatcaggcc ctttgacagg cgggtaagcg
     4441 atcgccaggc cctgttgccg gcagctttat ccgatggcag cgggttcatc tgtcctcttg
     4501 tcgcctcgca ccggtttgag aaaaacgcgc aaaaacacgt cgtctctcgt tcaacgaaac
     4561 tgaaatccac aacaagcaac tggaggaaac gcattatgaa actacgccct ggcgctttgc
     4621 acgccctacg catcccggcc gtcattgccg cccttttggc tttttcccat ctgcccgccg
     4681 cgcaggccgc cgacatgatc ggcaattgtg aggtcaccgg cgtcaagggt tccatgccgt
     4741 ttacgccggt taatgccggc gtgttgaccg tcgaggtgaa ccttccctca aatccgggct
     4801 ggtacaatgg cgacacgccg gaccagatca aggacggcta cgaatattgc ctggccgcca
     4861 acatcgccca ccgcgccggc cttgacggcg tgaagatcgt caatgtggcg tgggatgcgc
     4921 tcgtcaccgg ccataccaag gatttcgaca tcgcgctctc gcagatttcg atcaccgagg
     4981 aacgcaagaa agtggtcgat ttctcgacgc cttacttcaa ctcggatttc ggcgttctgg
     5041 tcaagaaggg caccacggtc gattcaacgt cgatcaagaa gatgcgcttt gccgtgcagc
     5101 agtcgaccac cggcgccgat ttcgtcacca acaaggtgca gccggccgaa gtcaaggtgt
     5161 ttcccgatgc gccgggcatg gtcacggccc tgatggcagg ccaggtcgaa gccgccggca
     5221 acgacaccgc agtcatgctc ggctgggcga cgcgctcgca gggaaagctt gaggtcgtcg
     5281 gccagtatac cacgggcgag gcatatggcg gcctttaccc caaggcgtcc cccaatgccg
     5341 cgaagatcaa cgagatcatc gaagccctga tcaaggacaa cacggtgaag tcgctgtcga
     5401 ccaaatatct cggtccggtt ctcggagccg atccgacctc cgtgccgtac atcaagccgt
     5461 gatcgcaagc agcgatcgat taaacaggta ctgacccgat gtcttttacc aaggatacaa
     5521 atgggggccg gaattccggc cccctcggcg gaaaacgctg gagcgccggc acgccgccaa
     5581 aggatgtcac cggttggatg gtcagcgcgc tgctcgtcac cgtcctggtc atcgccgcca
     5641 ccttcagaac ggctctcgat gtcagggccg ccgccgctac gctgggcgtc agcggccttg
     5701 ttgtcgatgc gatccttgtc gtcggctgcc tcaccgtcct gtcgctgttg cggccggcgc
     5761 tcaagagcat gcgccaggcg cgcgccgtca gttcggccct cgcgtccaat aatgtaatcg
     5821 acgcccgcgt cgccgccgct gcatccaggg attccgccta ttacacattg ggttccgccg
     5881 tggcggtcgt ggcggcgctg cttctcattc agttcatcat cgccaacaac cttgccgttg
     5941 gccgcacctt cttcctgctg cccctgatct ggagcaccat ctggcttgtc atcaaagcct
     6001 tctgggtcaa catctacatt ttcgtcgtcg ccgagatact agtgctgatc tggggcctga
     6061 tcgtcgcggt ggcgcggctg atgccgggtg acgccgggcg gccggtgcgg atgatcgcaa
     6121 cgatctatac ggacatgttt cgcggcctgc cggccatcat caccatctat ctggtgggct
     6181 ttggcctttc gctgaccgat ctgccctgga tcaaggatct gtcgcaggaa accttcgcca
     6241 tcatcgccct gaccctgacc catggcgcct atacggctga agtatatcgc gcgggcattc
     6301 aaagcattca tggcagccag gtcgcggcgg cgcgctcgct tggcctgtcc tacttccaga
     6361 ccctgcgata cgtcatcgtg ccgcaagcgg tgcgacgcat cgtcccgccg cttctgaacg
     6421 gcttcatcgg cctgcaaaaa gacacggcgc tggtgaacgt gatcggcgcg gtcgatgctt
     6481 tcaaccaggc gaaaatcctt accagcaatt acttcaacct gtcgccggtt accggggtcg
     6541 ccctcctgtt catactggtc accattccgc aggcgcgttt cgtcgacaag ttgatagagc
     6601 acgatcagcg tcgcacgcgc gccggcggca attgaggtca tccgactatg gcaatgatcg
     6661 aaatacgcga tatccataag cattacgggg cgtataaggt cttcgacggc ctcagtctcg
     6721 acgtcgagga acatcaggtc gtctgtctcg tcggggcttc cggctgcggc aagtcgaccc
     6781 tgctgcgcgc catgaacggc cttgagcaga tacagtccgg cgaaatccgc atcaacgccg
     6841 accggatcac cggtcccggc gtcgatgtca acgcgctgcg gcgcgatgtc ggcatcgtct
     6901 tccagagcta caatcttttc ccccacatga ccgtgctgca aaacgtcact ttggcgccca
     6961 tcaaggttct gggcgaggat cgtcacgagg cggaggcgag ggcgatatcg ttcctgaagc
     7021 ggatcggcct ggagcacaag gcgaacgaat atcccgacag gctttcgggc ggccagcagc
     7081 agcgcgtcgc cattgtccgg gccatggcga tgggaccgat ggtgctgctg ctggacgaga
     7141 tcacgtcggc gctcgacccg gaactcgtct cggaggttct gaatatcgtc cgcgatctcg
     7201 cggccgaagg catgacgatg ctgcttgcca cccatgagat ggcctttgcc cgcgaggtgg
     7261 cgagcaaagt ctgcttcctc gccgacggaa aagtgcatga ggaagggccg ccggaagaga
     7321 ttttcggcaa ccccaaacag gagcgcaccc ggacgttctt gcagcggatc atcgaggccg
     7381 ggcgacttta gaacgcggct ctgcctggtt cagggccggg cgctttcacg cctggcggca
     7441 tccggagggg catcctccta ggaaatttgg cgatcgtgaa gcatcgtagc gatataggag
     7501 agtgaccaaa ttggaattga ccgctgtggc cgagatcgac acccagaatt tccagccggc
     7561 gtcgcgcatc gcctccgtcg aggtgtcgga aattctcagg atcggcgcgc gcgcgcaagc
     7621 gctgaagaag gctggaagac cgatcatcgt tctgggggcc ggagagcctg atttcgatac
     7681 gcccgaccat gtcaagcagg cggcatcgga cgcgattgcg cgcggcgaga ccaaatacac
     7741 cgccctcgac ggtacagtgg cgcttaaaaa cgcgatccag cgcaagttct cgcgggaaaa
     7801 cgcggtcgaa tatgcgctgg acgagatcac ggttggggca ggcgccaagc agatattgtt
     7861 caacgccatg atggcgacgc tgaacgaggg cgatgaggtg atcatcccca cgccatattg
     7921 gacctcgtat tcggatatcg tccgcatcgc gcaaggcgtt ccggttttcg tgacctgcga
     7981 cgccgacgcc ggcttccgca tgacggcaaa acagcttgag gccgccatca cggcgaaaac
     8041 cagatgggtg atgctcaatt cgccttccaa cccttcgggc gcggcctaca gcgcggccga
     8101 ctatcggccc cttctggacg ttctgctcaa gcacccgcac gttcatctgc tggtcgacga
     8161 catgtatgag catatcgtct atgacggctt cgagttcgtg acgccggccg cactcgaacc
     8221 ccgcctgaag aaccgtatcc tgaccgtcaa cggcgtgtcc aaggcctatg cgatgaccgg
     8281 atggcgtatc ggatatgccg gcggccccaa ggccctcatc gaggcgatgg cggtggtgca
     8341 gagccaggcg acgtcatgtc catcctccgt cagccaggcc gccgccgtcg aggccttgaa
     8401 cggcacccaa gacttcgttg gggaacgctg caaaagtttt caggatcgtc gcgacctcgt
     8461 cgtggcgggc ctgaacgcaa tcgacggcat cgactgccgg gttcccgacg gcgcgttcta
     8521 caccttcgcc agttgcgccg gcctgctggg caggaagcgc cccaatggcg cgatcatcgc
     8581 gaccgacgcc gatttttgcg attacctttt gaccgaggcg gacgtggcgg tggtccccgg
     8641 ttcggcattt ggcttgtcgc cgtattttcg gatttcctac gccacctcca gcgatcagct
     8701 gaaggaagcg ctgcaaagga tgaagcaggc atgtgaacgc ttggcatagc ggttttgccg
     8761 aaaattcggc caatcggttt ttgacgttca aactgtgatc gctaccgcct tgaacggcga
     8821 aatcgaactg caatcgcccc gcccatcgta tccatcagac gaagggcggg gcgattgcgt
     8881 ttgcgaacgc tagaaggcgc atggttggga ggcttgtgtc atgc
//
LOCUS       DASYCS010000208         2444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_5124588,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000208 DASYCS010000000
VERSION     DASYCS010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5124588"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VGM46_06745"
     CDS             complement(<1..372)
                     /locus_tag="VGM46_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY4192328.1"
                     /translation="MSGRLTVIGLGPGNADQITPEARNAVAEAEFFYGYKPYVDRLDL
                     RESQIRVASDNREELARAKDALAKAAQGHRVAVVSGGDPGVFAMSAAVCEAIEAGPAE
                     WRAIDISFIPGITAMLAVAARI"
     gene            complement(369..1136)
                     /locus_tag="VGM46_06750"
     CDS             complement(369..1136)
                     /locus_tag="VGM46_06750"
                     /EC_number="2.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030788 - precorrin-2
                     C20-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-2 C(20)-methyltransferase"
                     /protein_id="HEY4192329.1"
                     /translation="MNDFPVNGRLIGVGTGPGDPELLTLKAVRALAEADVVAHFAKRN
                     ANGNARAIVDAHMKPGIVELPLYYPVTTEIDKDHDDYKAQILAFYEQSAGAVAAHLDA
                     GRTVAVLSEGDPLFYGSYMHLHVRLAHRFPTEVIPGVTAMSGCWSATSQPIVQGDDVL
                     TVLPGTMGESELTRRLSDTDAAVIMKVGRNLPKIRRALSASGKLDRAIYVERGTMANS
                     ASMRLIDKADDKAPYFTVILVAGWANRPGELASGVVL"
     gene            complement(1133..1765)
                     /locus_tag="VGM46_06755"
     CDS             complement(1133..1765)
                     /locus_tag="VGM46_06755"
                     /EC_number="5.4.99.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020052875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016993 - precorrin-8X methylmutase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HEY4192330.1"
                     /translation="MAAYDYIHDGTAIYERSFAIIRAEADLSRFSEAEADVAVRMIHA
                     CGLVEAARHFVFSQNFVAAARAALLSGAPILCDAEMVARGVTAARLPASNEVICTLRD
                     TATVERAREIGNTRSAAAIDLWGDRMAGAIVAIGNAPTALFYLLEKLRDGAPKPAAII
                     GMPVGFVGAAESKDALAENSYGVPYAIVRGRLGGSAMTAAALNALARPGL"
     gene            complement(1768..>2444)
                     /locus_tag="VGM46_06760"
     CDS             complement(1768..>2444)
                     /locus_tag="VGM46_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006335688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="precorrin-3B synthase"
                     /protein_id="HEY4192331.1"
                     /translation="PAGEPTSPAVEPNTQSPISVFNLINESLALGVGLPFGSMPAGKL
                     VALVRKSIELGCAEIRLAPGRALLFPGLCAATSQSLQMAAAELGFITKANDPRTRIAA
                     CPGAPACASGMIETRNIAEEIAATQMEFLDPSFTLHISGCAKGCAHPAAARLTLVGGE
                     KGAGLVVDGTAKSLPVGYRPRYDGARGVSRVAAAARAACQPGDTAASSLARLGAAAIA
                     QAYRQE"
BASE COUNT          416 a          733 c          864 g          431 t
ORIGIN      
        1 gatgcgggcg gcgacagcca gcatcgcggt gatgccgggg atgaaggaga tatcgatggc
       61 gcgccattcg gcgggaccgg cttcgatagc ctcgcagacc gccgccgaca tggcgaaaac
      121 gccggggtcg ccgccggaaa cgacagccac gcgatgccct tgcgcggcct tggccagcgc
      181 atccttggcg cgggcgagtt cctcgcggtt gtcggaagcg acgcggattt gactttcgcg
      241 caggtcgagc cggtcgacat agggcttgta gccatagaag aactcggctt cggcaaccgc
      301 gttgcgggct tcgggcgtta tctggtcggc attgcctggt ccaaggccga tgacggtcaa
      361 gcggccgctc acagcacgac cccggaagca agttcgcctg gccgattggc ccagccggct
      421 accagaatga cggtgaaata gggggccttg tcgtcggcct tgtcgatcag ccgcatggac
      481 gcgctgttgg ccatggtgcc gcgctcgaca tagattgccc tgtccagctt gcccgatgcc
      541 gaaagcgcgc gcctgatttt cggcaggttg cggcctacct tcatgatgac ggcggcatcg
      601 gtgtcggaaa gacggcgcgt cagttcgctc tcgcccatgg tgccgggcag gaccgtcagc
      661 acgtcgtcgc cctgcacgat tggctggctg gtcgccgacc agcagccgga catggctgtc
      721 acgccgggga tgacctctgt gggaaagcgg tgcgccagcc gcacatgcag gtgcatgtag
      781 gaaccataga aaagcggatc gccttccgac agcacggcaa ccgtgcggcc tgcatcgaga
      841 tgcgcggcga cggcgcccgc cgactgctcg tagaaagcca ggatctgcgc cttgtagtcg
      901 tcgtggtcct tgtcgatttc ggtggtgacc ggataataaa gcggcagttc gacgatgccg
      961 ggcttcatat gggcgtcaac aatcgcgcgg gcgttgccgt tggcgttgcg tttggcgaaa
     1021 tgcgccacca catcggcctc ggccagcgcg cgcacggctt tcagggtcaa aagttcaggg
     1081 tcgccggggc cggtgccgac gccgatcagc cggccgttga cgggaaaatc attcataggc
     1141 cgggtctcgc caaagcgttg agggcggcgg cggtcatggc gctgccgcca agcctgccgc
     1201 gcacgatggc gtaggggacg ccgtaggagt tttctgccag cgcgtccttc gattcggccg
     1261 cgccgacgaa gccgaccggc atgccgatga tggcggcggg cttgggcgcg ccgtcgcgca
     1321 atttttccag aaggtagaac agagcggtcg gcgcattgcc gatggcgacg atcgcgcccg
     1381 ccatgcggtc tccccacagg tcgatggcgg cggcggaacg cgtgttgccg atctctctcg
     1441 cgcgctcgac ggtcgccgta tcgcgcaacg tgcaaatcac ttcgttcgat gctggaaggc
     1501 gggcggcggt gacacctcgc gccaccattt cggcatcgca aaggatgggc gcgccggaga
     1561 gcaaggcggc gcgggcggcg gcgacaaagt tttgcgagaa aacaaagtgc cgggcggcct
     1621 cgaccagtcc gcaggcgtgg atcatcctga cggcgacgtc ggcttcggct tcggaaaagc
     1681 gggagaggtc ggcctcggcc cggatgatgg cgaacgagcg ctcgtagatc gcggtgccgt
     1741 cgtggatgta gtcgtaggcg gccattttca ttcctgtcga tacgcttgcg ctatggcggc
     1801 ggcacccagg cgcgcgaggc tcgatgctgc cgtgtcgccc ggctggcatg cggcgcgcgc
     1861 ggctgctgcg acgcggctga cgccgcgcgc gccgtcatag cgtggcctgt agccgacagg
     1921 aaggcttttt gccgttccgt caacgacaag gccggctcct ttttcgccgc cgaccagcgt
     1981 cagcctggcg gcggccggat gggcgcagcc ttttgcgcag ccggaaatat gcagcgtgaa
     2041 ggaaggatcg aggaattcca tctgcgtggc ggcgatttcc tcggcgatgt tgcgcgtttc
     2101 gatcatgccg gaggcacagg ccggcgcgcc ggggcaagcc gcaattcttg tgcgtggatc
     2161 attggccttg gttatgaagc ccagttcggc cgccgccatt tgcagcgatt gggaagtggc
     2221 tgcgcaaagg cctggaaaaa gtagcgcccg gcccggcgcg aggcgaattt cagcgcagcc
     2281 gagttcgatg gattttcgga ccagggcaac aagtttgccc gccggcatgg aaccgaaggg
     2341 caggccgacg ccgagggcaa ggctttcatt tatgaggttg aagacggaga tgggagattg
     2401 cgtattgggc tcgacagccg gacttgtcgg ctcacccgcc ggca
//
LOCUS       DASYCS010000209         7249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2513894,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000209 DASYCS010000000
VERSION     DASYCS010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 7249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7249
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2513894"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..284)
                     /locus_tag="VGM46_06765"
                     /pseudo
     CDS             complement(132..284)
                     /locus_tag="VGM46_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019170562.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
     gene            complement(465..698)
                     /locus_tag="VGM46_06770"
     CDS             complement(465..698)
                     /locus_tag="VGM46_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192332.1"
                     /translation="MTPTIALVWYMVVAGPQGGMVVLPSTFDTRDQCTSAVTEYQKTP
                     APAGWTVQCIPSASPYSDDLNEGDDSAPAQPAQ"
     gene            complement(860..1504)
                     /locus_tag="VGM46_06775"
     CDS             complement(860..1504)
                     /locus_tag="VGM46_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192333.1"
                     /translation="MRKLLLLTALTIATGGAALAQDNSPAQDNSMSFFVTSQGSGKGA
                     DLGGLAGADALCDTRAVVAGVTGKTWHAYLSTSDTDARDRIGKGPWFNAKGEKIADDV
                     AQLHSDANNLTKQTALNEKGEVINGRGDTPNRHDILTGSKPDGTKIADQTCGDWTMSG
                     TEGAAMMGHSDRTGLDDSASAKSWNSSHASRGGCSQEALKGTGGDGLLYCFVAK"
     gene            complement(1621..2388)
                     /locus_tag="VGM46_06780"
     CDS             complement(1621..2388)
                     /locus_tag="VGM46_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006331493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY4192334.1"
                     /translation="MTPALDLTGRTALVTGASRGIGYFLAKQLAAAGAHVIAVARTVG
                     GLEELDDEIKAEHGRTGKGEATLAPLDLTDMEGIDRLGGAIHQRWGKLDILVANAGIL
                     GVISPIGHVEAKTFEKVMTINVTSTWRLIRSVDPLLRLSDAGRAIVLSSNAAHTAKAF
                     WAPYAASKAAVETMTRSWAAETESMALRVNAADPGATRTGMRAQAMPGEDPQTLPHPS
                     EVAARIVPMASPSLSRTGQIYQAKHDRWVSYRQPEEA"
     gene            2576..2980
                     /locus_tag="VGM46_06785"
     CDS             2576..2980
                     /locus_tag="VGM46_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY4192335.1"
                     /translation="MSKTVALLVARLIFAGMFAMAATFKFSGMADTASYIAAAGFPFP
                     LLLAWLAAIFEVALVLAFLTGAFFSEAALLAAVYVIFLAFAFHGPSRWQANQGEFGFF
                     VDHFTFLAGLLFAAVHGPGDRLAIRCGLIWRR"
     gene            complement(3022..4491)
                     /gene="purF"
                     /locus_tag="VGM46_06790"
     CDS             complement(3022..4491)
                     /gene="purF"
                     /locus_tag="VGM46_06790"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018427099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEY4192336.1"
                     /translation="MADADTALCAEADDHFHDECGVFGIFGRQDAGAIVTLGLHALQH
                     RGQEAAGIVSYDGTQFHVERHVGLIGDTFTKQRVIDSLQGDRAIGHTRYATTGGAGVR
                     NIQPFFAELADGGFAVAHNGNLTNAMTVQRRLQKQGSIFSSTSDTETILHLVATSKER
                     DLNARFIDAVRQVEGAFSLVAMSSKKMIGCRDPLGIRPLVLGDLDGAWILASETCALD
                     IIGARFVRDVEPGEMVVVTTDGIESLFPFAPQKTRFCIFEYVYFARPDSSVEGRNVYD
                     VRKRIGAELSRESPVEADIVVPVPDSGTPAAIGFSQEAGIPFELGIIRNHYVGRTFIS
                     PGDSIRHMGVKLKHNANRRVIEGKRVVLVDDSIVRGTTSQKIVQMVRDAGAREVHMRI
                     ASPPTRASCFYGVDTPEKSKLLASRMSIEEMAEFIRVDSLGFLTIDGLYRAVGEAGRN
                     SEQPQFCDACFTNQYPTRLADFEGHDNVRTLSLLATGGM"
     gene            complement(4577..5218)
                     /locus_tag="VGM46_06795"
     CDS             complement(4577..5218)
                     /locus_tag="VGM46_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEY4192337.1"
                     /translation="MPITLLDGILVGFTLVSAMLAMVRGFSREVLSVVSWAAAAAAAY
                     FFYKPVIPYVQPYIDNDKAAMAAAAGIVFIIALIVVTVITMKLADWIIDSRIGALDRT
                     LGFLYGAARGILVVAVALMFFNWLAGDKAPNWIASAKSRPLLESIGAKIESLLPEDPE
                     NAILKRLSPESAAPQPGTETAPTTEAPATGDDANTPAPDDSEEPAPDAPQPAN"
     gene            complement(5243..6658)
                     /gene="radA"
                     /locus_tag="VGM46_06800"
     CDS             complement(5243..6658)
                     /gene="radA"
                     /locus_tag="VGM46_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEY4192338.1"
                     /translation="MAKSRVQFICQNCGSVHQRWAGKCDACGEWNTLVEEGTAGGIGS
                     GPASTRNARKGRAVVLTTLSGEIEDAPRIISGIGELDRATGGGFVRGSALLVGGDPGI
                     GKSTLLTQAAAALASKGHRIVYVSGEEAVAQIRLRAQRLGVADTSVELAAETNVEDIL
                     ATIADGKRPDLVILDSIQTLWTDLADSAPGTVTQVRAAAQAMIRYAKSTGAAIVLVGH
                     VTKEGQIAGPRVVEHMVDAVLYFEGEGGHHYRILRTVKNRFGPTDEIGVFEMSDKGLR
                     EVANPSELFLGERHAKSPGAAVFAGMEGTRPVLVEIQALVAPSSLGTPRRAVVGWDGA
                     RLSMILAVLEAHCGVRFGQHDVYLNVAGGYRITEPAADLAVAAALVSSLTGLALPADC
                     VYFGEISLSGAVRPVAHAQQRLKEAEKLGFGSAVLPLGSSKGSEEVAGGIGAGAFRPT
                     ELADLVARIAGTRRSGSAGED"
     gene            complement(6662..>7249)
                     /locus_tag="VGM46_06805"
     CDS             complement(6662..>7249)
                     /locus_tag="VGM46_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006331503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HEY4192339.1"
                     /translation="LVACSQTMQKIPLFIDQTGGISIAQLSARARRLKRQRGLDVIVI
                     DYIQLMQGSSAKSSQNRVQEITEITTGLKALAKELGVPIIALSQLSRQVESRDDKRPQ
                     LSDLRESGSIEQDADVVMFVFREEYYLKNKEPKPGTEEYIKWEMEMNEMRGKAEVIVA
                     KQRHGPTGSVSLAFQGEFTRFSDLAEEHHIAERFE"
BASE COUNT         1302 a         2271 c         2299 g         1377 t
ORIGIN      
        1 tgtttttcat gaagaaaacc gccattcggc tggcgtcgag aagaacagac caagtgtgga
       61 gggactggag gggcggctcg caacccacat gatgcccgca gtcgcaaagc gtcccgttcg
      121 gccggacttt ttcaacgatc cgggcttttg gtgactgtcc ttgccgccag ccagtcgcgg
      181 ccggcatggg cgaataccgc gaggatcacg atggcgccgc cccacatgct ggcggccggt
      241 ggaatctcgg acaggaagag gaaggcgaaa agaatggcga agggaaagag ccagccatgc
      301 gcgcaatccc gtttcaggag actttatccg gtcggtcctg acaccatgcc ttcagcaata
      361 tccggcctga actcgatctt cacagcttga aaccgggcat aggccagcgc cgtggccatc
      421 gatggcagcc acggatcgtc ggtttggcgt gtggagcgag ccgcttactg agccggttgt
      481 gccggcgcgg aatcgtcgcc ttcgttcaga tcgtcggaat agggcgaggc gcttggaatg
      541 cactgtacgg tccatcctgc cggcgccggc gtcttttgat attcggtgac ggcggaagtg
      601 cactggtcgc gcgtatcgaa cgtgctcggc agcacaacca tgccgccttg cggtccggcg
      661 accaccatat accagaccag ggctatcgtc ggtgtcataa aatcctcaca tgcacgaatg
      721 ggaaatcggc tagtgcgcaa gctttagcgc atcggcgcga aaaccggaat cgattttcgc
      781 gccggcaggt gagaaataga gatcgcaaaa accatgcccg gcgaccgccg cttatatggg
      841 ccggcccatg gtttttggcc tatttcgcga cgaagcaata aagcaggccg tcgccgcccg
      901 ttcccttcag cgcttcctgg ctgcaaccgc cgcgcgaggc atgcgaggaa ttccatgact
      961 tggccgatgc cgaatcatca agcccggtcc ggtcggaatg tcccatcatg gcagcacctt
     1021 ccgtgccgct catcgtccag tcgccgcagg tctggtcggc gatcttggtg ccgtccggct
     1081 tcgagccggt caggatgtcg tgccgattgg gcgtgtcgcc gcgtccattg atgacctcgc
     1141 ccttctcgtt cagcgccgtc tgcttggtca ggttgttggc gtccgaatga agctgggcca
     1201 cgtcatcggc gatcttttcg cccttggcgt tgaaccacgg gcctttgccg atgcggtcgc
     1261 gggcatcggt atcgctggtc gagagatagg cgtgccaggt cttgccggtt acgccggcca
     1321 ccacggcgcg cgtgtcgcaa agcgcatcgg cgccggccag ccctccgaga tcggcgccct
     1381 tgccggaacc ttggctcgtc acgaagaaac tcattgaatt gtcttgggca ggggaattgt
     1441 cttgggcaag ggccgcgcca ccggtagcaa tggtcagggc agtcaagagc agcaattttc
     1501 gcatgatggt tgtcctccag ctcgtgttga cggatgtagc ttgctggaaa acgatagttg
     1561 gcgccgaaat agtccaccaa tcacgagatt gtgtggttaa aaaacgttta ccgcgccggc
     1621 tcaagcctct tccggctgcc ggtaggaaac ccagcggtcg tgcttggcct ggtaaatctg
     1681 tccggtcctg gacagcgaag ggcttgccat cggcacgatg cgggcggcga cttccgaagg
     1741 atgcggcagc gtttgcggat cttcgcccgg catggcctgc gcgcgcattc ctgtccgtgt
     1801 cgcgcccgga tcggcggcgt tgacgcgcag cgccatgctc tcggtctccg ccgcccagga
     1861 gcgcgtcatc gtctcgaccg ccgccttgga agccgcatag ggcgcccaga acgccttggc
     1921 cgtatgcgcc gcgttggacg acaaaacgat ggcgcgtccg gcatccgaaa gccgaagcag
     1981 cgggtcgacc gagcggatga gccgccaggt cgaagtaacg ttgatggtca tcaccttttc
     2041 gaaggtcttg gcttccacat ggccgatagg cgagatgacg ccgagaatgc ctgcattggc
     2101 caccagaatg tcgagcttgc cccagcgttg atggatcgcg ccgcccagcc ggtcgatgcc
     2161 ctccatgtcg gtaaggtcaa gcggcgccag cgtcgcttcg cccttgccgg tcctgccgtg
     2221 ttcggccttg atctcgtcgt ccagttcttc cagcccgccc accgtgcgcg caaccgcgat
     2281 gacatgcgcg cccgccgctg caagctgctt ggcgagaaaa tagccgatgc cgcgcgatgc
     2341 gccggtaacg agggcggtac ggccggtaag gtcgagggca ggggtcatgc gctggcgtcc
     2401 tgtgtgggca agtgatagct ggcaccagat aggccgaatc cgctcatggc gaaacccttc
     2461 aaagcgcgat tgccgtggcg tggatgtgga gtaattttct tgggcaagcc tgtgtcggga
     2521 atgagcgttc cccggcgtcc ttggtcagga atgaaataaa agctggagaa gaaccatgag
     2581 caaaaccgta gcgcttctcg tcgccaggct gatttttgcc ggcatgtttg cgatggccgc
     2641 caccttcaag ttttccggga tggccgacac cgccagctat atcgcggcgg ccggatttcc
     2701 gttcccgctg ctactggcct ggcttgccgc catcttcgag gtcgcgctgg tgcttgcttt
     2761 cctcaccggc gctttctttt cggaagcggc gctactggcg gcggtctatg tgatttttct
     2821 ggccttcgcc ttccatggtc cgtcgcgctg gcaggcgaac cagggcgaat tcggcttctt
     2881 cgtcgaccat ttcacttttc tggccggatt gctgttcgcc gccgtccatg gtccgggcga
     2941 ccggctggcc attcgctgcg gtctcatctg gcggcgctga attcacccgg cgccggccag
     3001 ctttcttgag aaaaacgagg cctacattcc gcccgtcgcc agcagcgaca gcgtgcggac
     3061 attgtcatgc ccttcgaagt cggcaagccg cgtcggatac tgattggtga agcaggcatc
     3121 gcagaattgc ggctgttcgc tattgcgccc ggcttcgccg accgcgcgat aaagcccatc
     3181 aatggtgagg aagccaagcg aatccacccg gatgaactcg gccatctcct cgatcgacat
     3241 gcgcgaggca agcagtttcg atttttccgg cgtatcgacg ccatagaagc atgaagcccg
     3301 cgtcggcggc gaagcgatgc gcatatgcac ttccctcgcg ccggcatcgc gcaccatctg
     3361 cacgatcttc tggctggtgg tgccgcgcac gatggaatca tcgaccagca ccacgcgctt
     3421 gccctcgatc acccggcggt tggcgttgtg cttcagcttg acgcccatgt ggcggatcga
     3481 atcgccgggc gaaatgaagg tgcgcccgac atagtggttg cggataatgc cgagttcgaa
     3541 cggaatgcct gcttcctgcg aaaaaccgat tgccgccggc gtacccgaat ccggcaccgg
     3601 cacgacgatg tcggcttcca ccgggctttc gcgcgaaagc tccgcgccga tgcgcttgcg
     3661 cacatcatag acgttgcggc cctcgaccga ggaatccggt cgcgcgaaat acacatattc
     3721 gaaaatgcag aaccgggtct tttgcggcgc gaaaggaaac aggctttcga tgccgtcggt
     3781 ggtgacgacc accatctcgc ccggctcgac gtcgcgcacg aaacgcgcgc cgataatgtc
     3841 gagcgcgcaa gtctcggaag cgagtatcca ggcgccatcg aggtcgccaa gcaccagcgg
     3901 ccggatgccg agcgggtcgc ggcagccgat catcttcttg gaagacatgg cgaccagcga
     3961 aaaagcccct tcgacctggc gcaccgcatc gatgaagcgc gcattgagat cgcgctcctt
     4021 gctggtcgcg accagatgca ggatcgtttc ggtgtcggag gtggacgaga agatcgagcc
     4081 ttgtttttgc agccttcgct gcaccgtcat cgcattggtg aggttgccat tatgggcgac
     4141 ggcaaagccg ccatcggcaa gctcggcgaa aaacggctgg atgttgcgaa cgccggctcc
     4201 gccagtggtg gcatagcgcg tatgcccgat ggcgcggtcg ccttgcaggc tgtcgatgac
     4261 gcgctgcttc gtgaaggtgt cgccgatcag gccgacatgg cgctccacat ggaactgggt
     4321 gccgtcatag gagacgatgc cggcggcctc ctggccgcga tgctgaagcg cgtgcaggcc
     4381 gagcgtgacg atggcgcccg catcctggcg tccgaaaata ccgaacacgc cgcactcgtc
     4441 gtggaagtgg tcatccgcct cggcgcatag cgctgtatct gcatctgcca ttgggctgag
     4501 ctccgctaaa atcgctatat aaggcaccag tgggccagga acaacgccaa caattcgtgg
     4561 tcctgtttaa tcttcgtcag tttgccggct ggggcgcgtc gggcgctggc tcttcgctgt
     4621 cgtcaggagc tggcgtattg gcgtcgtcac cggtcgcggg cgcttcggtc gtcggcgcgg
     4681 tttccgttcc cggttgcggc gcggccgatt cagggcttag ccgcttgagg atcgcgtttt
     4741 ccgggtcttc cggcagcagg ctctcgatct tggcgccgat cgattcgagc aacggcctcg
     4801 acttggccga agcgatccag ttgggggcct tgtcgcccgc cagccagttg aagaacatca
     4861 aagccacggc gacgacgagg atgccgcgtg ccgcgccgta aaggaagccc agcgtgcggt
     4921 cgagcgcgcc gatccgcgaa tcgatgatcc agtcggcaag cttcatggtg atgaccgtca
     4981 ccacgatcag cgcgatgatg aaaacgatgc cggcggctgc cgccatcgcc gccttgtcat
     5041 tgtcgatata gggctgaacg taggggatga ccggcttgta gaagaaatat gccgctgccg
     5101 ccgccgccgc ccatgaaacc acggacagaa cctcgcggga aaagccgcgt accatggcca
     5161 gcatcgccga aaccagggtg aagccgacca gaattccgtc aagcagtgta atcggcatgt
     5221 ttcgcgcccc actccgtgtc catcagtctt ccccggcgga gccgcttcgg cgtgtgccgg
     5281 cgatgcgcgc cacaagatcg gcaagctcgg tgggccggaa cgcaccggct ccgattccgc
     5341 cggcgacttc ctcgctgccc ttcgagcttc ccaaaggcag gacagcactt ccaaaaccca
     5401 gcttttcggc ttccttgaga cgctgctgcg catgcgcaac cggcctcacg gcgcccgaca
     5461 ggctgatttc gccgaaataa acgcaatcgg ccggaagggc aaggccggtc agcgaggaaa
     5521 ccagggccgc cgccaccgcc aggtcggcgg caggctcggt gatgcgatag ccgccggcga
     5581 cgttcagata gacgtcgtgc tggccaaacc gcaccccgca atgggcctcc agcaccgcca
     5641 ggatcatcga aagccgggcg ccgtcccagc cgaccacggc gcggcgcggc gtgccgagcg
     5701 aggagggcgc gaccagcgcc tggatttcca ccagcaccgg gcgcgtgcct tccatgccgg
     5761 cgaaaacggc ggctcccggc gatttcgcat ggcgctcgcc cagaaaaagc tcggacggat
     5821 tggcgacttc acgcaggccc ttgtccgaca tttcgaacac gccgatctcg tcggtcggtc
     5881 cgaaccggtt cttgacggtg cgcaagatgc ggtagtgatg gccgccttcg ccctcgaaat
     5941 agagcacggc gtcgaccatg tgctcgacca cgcgcggccc ggcgatctgg ccttccttgg
     6001 tgacatggcc gaccaaaacg atcgccgcgc ctgtggattt cgcatagcgg atcatcgcct
     6061 gcgccgccgc ccgcacctgc gtgaccgtgc cgggggccga atcggcaagg tctgtccaca
     6121 gcgtctggat ggaatcgagg atgacgaggt cgggccgctt gccgtcggca atcgtcgcca
     6181 gaatgtcctc cacattggtt tcggcggcca gttccacgga agtgtcggca acgccgaggc
     6241 gctgggcgcg caggcggatt tgcgcgaccg cctcctcacc ggagacgtag acgatgcggt
     6301 ggcccttgct cgccagcgcg gccgccgcct gtgtcagcag tgtcgacttg ccaatgccgg
     6361 ggtcgccgcc gaccagcagc gccgagccgc gcacgaagcc gccgccggtg gcgcggtcca
     6421 gttcgccgat gccggaaatg atgcgcggcg catcctcgat ctcgcccgaa agcgtggtga
     6481 gcacgaccgc gcggcccttg cgcgcattgc gcgtcgaggc ggggccggag ccgataccgc
     6541 cggcggtgcc ttcctcgaca agcgtgttcc actcgccgca ggcatcgcac ttgccggccc
     6601 aacgctgatg cacggagccg caattctggc aaatgaactg aacgcgcgac ttggccaaga
     6661 gctactcgaa ccgttccgcg atgtgatgtt cttcggcaag gtcggaaaag cgcgtgaatt
     6721 caccctggaa cgcgaggctg acggaacccg tggggccgtg gcgctgcttg gcgacgatca
     6781 cctcggcctt gccgcgcatc tcgttcattt ccatttccca cttgatatat tcctcggtgc
     6841 cgggcttcgg ctccttgttc ttgaggtaat attcctcgcg gaacacgaac atcaccacgt
     6901 cggcgtcctg ctcgatggaa cccgattcgc gcagatcgga gagctgcggg cgcttgtcgt
     6961 cgcggctttc gacctggcgc gacagctgcg acagcgcgat gatcggcacg ccgagttcct
     7021 tggccagagc cttcagcccg gtggtgatct cggtgatttc ctgcacgcgg ttttgcgagg
     7081 acttggccga cgacccctgc atcagctgaa tatagtcgat gacgatgacg tcgaggccgc
     7141 gctggcgctt cagccgccgc gcccgcgccg aaagctgggc gatggagatg ccgccggtct
     7201 gatcgatgaa cagcgggatt ttctgcatgg tctgcgaaca ggcgaccag
//
LOCUS       DASYCS010000210         4630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_194645,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000210 DASYCS010000000
VERSION     DASYCS010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4630
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194645"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1816
                     /gene="addB"
                     /locus_tag="VGM46_06810"
     CDS             <1..1816
                     /gene="addB"
                     /locus_tag="VGM46_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="double-strand break repair protein AddB"
                     /protein_id="HEY4192340.1"
                     /translation="PVGLLALLKHPLLTVGLERPGVRHAAEVIELVALRGGTGRPDIA
                     TLPELFEERLTAAGSTKREPFWLTRLSGRDFEQARALLARLADIFAPLVALREAADAD
                     LLTLVRASILALEGLGRAADDSLGRLYEGDAGDKLAELLRGLVSASAPFSLAAAEWPD
                     VMEALIAPETVKPSRGGDKAIAIWGALEARLQSVDTLVIGGLNEGVWPRKAQSDRFMS
                     RLMKTDIDLEPPERRIGLAAHDFQMAMGAPRVVLARAARSGDAPAVPSRWLQRLLTFI
                     GKEHSAGMRRRGDELLSWARELDAGEKRDFAPRPRPTPPVAMRPQHFSVTEIETLRRD
                     PYAVYARRILGLTALDPVIRDPGAAERGTLFHAILHLFSRSIASPRDPSALEILLAAG
                     RSCFAEAALPADVEAVWWPRFEKLAVNIIEWELTRADAVARRLAEERAEKTMVGHSGV
                     TLSGYADRVDLLAGGMADILDYKTGSSPSKAQAHTLLAPQLALEGALLRRGAFRELGA
                     REPSQLAFVRLKPNGAVLEESILEYNRKPRSAAELAEEAWDRLEKLLIHYHDPATGYL
                     SRALPFREGETDGDYDHLARVLEWSAGGDAPEEGSGEG"
     gene            1813..>4630
                     /gene="addA"
                     /locus_tag="VGM46_06815"
     CDS             1813..>4630
                     /gene="addA"
                     /locus_tag="VGM46_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015314365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="double-strand break repair helicase AddA"
                     /protein_id="HEY4192341.1"
                     /translation="MKRNYPIPHETASNQARASDPRQSAWVSANAGSGKTHVLAQRVI
                     RLLLGGTDPSKILCLTYTRAAAANMSNRVFSTLSEWTTLDNDELAAKIETLNGKAPDQ
                     ALMRRARRLFAEALETPGGLKILTIHAFCESVLHQFPLEANIAAHFEMLDPQMEAALF
                     ATARRDMISGASGGNAELANAFATILERAGEAGLDALLGEIVRKRDGLRAFIGQVGGD
                     GSGFQALFDAFGFPAGQGVDEIAQAAWPLPGLEPEYLSRLEQACYRLGANRAQEYLVA
                     GATQAFAETDPVRRLGLMKKAFVSEKGEPYKPSWLFSKALLGDLPDLPDRYLAATEAV
                     IAAGDRLALYRMLEGTRAALTAADWLIGRYEQMKRGRGLLDFNDLITRTVNLLNRTDA
                     GPWVQYKLDQGIDHILLDEAQDTSPDQWQVVTRLAEEFFAGQGARNGAARTVFAVGDE
                     KQSIYSFQGAAPDSFHNSRVFFAMKVREAEADFADLKLSLSFRSTQDVLSAVDLVFAD
                     PQVRRGISHDADAPRHTAIRHDAPGYVELWPSVGADTVDEPDDWTLPVNHLRAPAVLV
                     AERVADTIQHWLKSGNSIEGKGRPVRAGDILVLVRKRDRFVHALSRSLKNRNIPMAGA
                     DRLSLPGHIAVKDMIALGRFLIQPQDDLSLAAVLRSPIFDVSEETLFELAGGRGKSVS
                     LIASLRHAGDDAGLAAVVKQLDGWASEAAFKPVFEFYAGLLARDGVRNRMIERLGLEA
                     GDILDEFLSFCLAEERTGLPGLETFLATLENAGPEIKREMDQGRDEVRVMTVHAAKGL
                     EAPVVFLVDGGSAPFSDQHLPRLMPFEGPGGDWRGKGYLWRAAGDVANEFSQSAAKLA
                     RDLADDEYRRLLYVGMTRAEDRLVVCGYHGKRAPNAGTWHSIVSRALVGAPGSSERRH
                     PVTDEPIYRFQVTNLPPVSPEPVEA"
BASE COUNT          757 a         1491 c         1613 g          769 t
ORIGIN      
        1 tccggttggc ctgcttgcct tgctcaagca tccgctgctg acggtcggac tggaacggcc
       61 gggcgttcgc cacgcggcgg aagtgatcga actggtcgcg cttcgcggcg gcaccggccg
      121 cccggatatc gccacgctgc ccgaactgtt cgaggaaagg ctgacggcgg ccggttccac
      181 caagcgcgag cctttttggc tcacgcgcct gtcgggccgg gatttcgagc aggcgcgcgc
      241 gctgcttgcc cgccttgccg acattttcgc gcccctggtc gcgctgcgcg aggcggcgga
      301 tgccgacctt ctaacactgg ttcgggcaag catcttggcg ctggaaggcc ttggccgcgc
      361 cgccgatgac agccttggcc gcctctatga gggcgatgcg ggcgacaagc tggcggagct
      421 tctgcgcggg ctggtgtcgg ccagcgcgcc gttctccctt gcggcggcgg aatggccgga
      481 tgtgatggaa gcgctgatcg cgccggaaac ggtcaagccg tcgcgcggcg gcgacaaggc
      541 gattgccatt tggggtgcgc tggaggcgcg cctgcaaagt gtcgatacgc tggtcatcgg
      601 cgggctgaac gagggcgtgt ggccgcgcaa ggcgcagagc gaccgcttca tgtcccggct
      661 gatgaagacc gatatcgacc ttgagccgcc ggaacggcgc atcgggcttg ccgcgcatga
      721 tttccagatg gcgatggggg cgccgagggt cgtgctggcg cgcgccgcac gctccggcga
      781 cgcaccggcg gtgccatcgc gctggctgca acgcctgctg acctttatcg gcaaagagca
      841 ttcggccggg atgcgccggc gcggcgatga actgctttcg tgggcgcgcg aactggatgc
      901 gggcgaaaag cgtgatttcg cgccgcgccc gcggccgacg ccgccggtgg cgatgcgccc
      961 gcagcatttc tccgtcaccg agatcgaaac gctgcgccgg gacccttacg ccgtctatgc
     1021 gcggcgtatc cttggcctga cggcgcttga cccggtgatc cgcgatccgg gcgccgccga
     1081 gcgcggcacg ctgttccatg ccatcctgca cctgttttcg cgcagcatcg ccagtccgcg
     1141 cgacccatcg gcgctggaaa tattgctcgc cgccgggcgc agctgctttg ccgaagctgc
     1201 cctgcccgcc gatgtcgaag ccgtgtggtg gccgcgcttc gagaagcttg ccgtcaacat
     1261 catcgagtgg gaattgacgc gggccgacgc cgtggcgcgc cgccttgcgg aggaacgcgc
     1321 ggaaaaaacc atggtcggcc attcgggcgt gacgctgtcg ggctatgccg accgggtcga
     1381 cctgctggcc ggcggcatgg ccgacatact ggactacaag accgggtcct cgccttcgaa
     1441 ggcgcaggcg catacgctgc ttgctccgca actggcgctt gaaggggcgc tgctgcggcg
     1501 cggcgcgttc cgggagcttg gcgcgcgtga gccctcgcaa ctggccttcg tgcggctgaa
     1561 gccgaacggc gcggtgctgg aggaatccat cctggaatat aaccgcaagc cgcgcagcgc
     1621 cgccgagctg gcggaggaag cgtgggaccg gctggaaaag ctgcttatcc actaccacga
     1681 cccggcgacc ggttatctgt cgcgggcgct gccgtttcgg gaaggcgaga cggatggcga
     1741 ttacgaccac ctggcgcgcg tgctggaatg gtcggccggc ggcgacgcgc ccgaagaggg
     1801 aagcggcgag ggatgaagcg aaactatccg atcccccatg aaaccgcgtc caaccaggcg
     1861 cgcgcttccg acccgcgtca gtcggcctgg gtgtcggcca atgccggttc cggcaagacc
     1921 catgtgctgg cgcagcgcgt tatccgcctt ttgctgggtg gcaccgatcc atccaagata
     1981 ttgtgcctga cctatacgcg cgccgccgcc gccaacatgt cgaaccgggt cttttcgacg
     2041 ctttcggaat ggaccacgct ggacaacgac gagctggcgg cgaagatcga gacgctgaac
     2101 ggcaaggcgc cggaccaggc gctgatgcgg cgggcgcggc ggctgttcgc cgaggcgctg
     2161 gaaacgccgg gcggcctgaa aatcctgacc atccatgcgt tttgcgaatc cgtgctgcat
     2221 cagttcccgc tggaagccaa catcgcggcg catttcgaaa tgctcgaccc gcagatggag
     2281 gcagcacttt tcgccacggc gcggcgcgac atgatttcag gcgccagcgg cggcaatgcc
     2341 gaactggcga atgccttcgc caccattctc gaacgcgccg gcgaggcggg gctggatgcg
     2401 ctgctgggcg agatcgtgcg caagcgcgac gggttgcgcg ctttcatcgg ccaggtcggc
     2461 ggcgacggtt ccggctttca ggcgctgttc gacgcgttcg gctttcccgc cgggcagggc
     2521 gtcgacgaga tcgcccaagc ggcatggccg ctgccgggcc tggagccgga gtatctaagc
     2581 cggctggagc aagcctgcta ccggttggga gccaatcggg cacaggaata tctggtcgcc
     2641 ggcgcgacgc aggctttcgc cgagacagat ccggttcgcc ggcttggctt gatgaagaag
     2701 gctttcgtca gcgagaaggg cgaaccgtac aagccatcct ggctgttcag caaggcgctg
     2761 ctcggtgacc tgcccgattt gccggaccgc tatctggcgg cgaccgaggc ggttatcgcg
     2821 gccggcgacc ggctggcgct atatcgcatg ctggaaggca cacgggccgc gctgacggcc
     2881 gccgactggc tgatcggccg ctacgagcag atgaagcgcg ggcgcggcct gcttgatttc
     2941 aatgatctca tcactcgcac ggtcaatctg ttgaaccgca ccgacgccgg gccgtgggtg
     3001 cagtacaagc tcgaccaggg catcgaccac atactgctcg acgaggcgca ggacaccagc
     3061 cccgaccaat ggcaagttgt aacgcggctt gccgaggagt tctttgccgg acagggcgcg
     3121 cgcaacggcg cggcgcgcac ggtattcgcg gtgggcgacg aaaagcagtc gatctattcc
     3181 ttccagggcg cagcgcccga ttcctttcac aacagccgcg tcttcttcgc catgaaggtc
     3241 agggaggcgg aagccgattt cgccgatctc aagctgtcgc tttcatttcg ctcgacgcag
     3301 gacgtgttgt cggcggtgga cctcgtcttt gccgatccgc aggtgcggcg cggcatcagc
     3361 cacgacgccg atgcgccccg ccacaccgcc atccgccacg atgcgccggg ctatgtcgaa
     3421 ctatggcctt cggtcggggc cgatacggtg gacgaacccg acgactggac gctgccggtc
     3481 aaccacctgc gcgcgccggc cgttctggtg gccgagcgcg tggccgacac catccagcac
     3541 tggctaaagt ccggcaacag catagagggc aagggccggc cggtgcgggc gggcgacatt
     3601 ctggttctgg tgcgcaagcg cgaccgcttc gtccacgcgc tgtcgcgcag cctgaagaac
     3661 cgaaacatcc cgatggccgg ggccgaccgg ctgagcctgc ccggccatat cgcggtcaag
     3721 gacatgatcg cgcttggacg cttcctcatc cagccgcagg acgatctttc gctggccgcc
     3781 gtgctgcgca gcccgatctt cgatgtttcc gaagagacgc ttttcgaact ggcgggtgga
     3841 cgcggaaaaa gcgtgtcgct catcgcatcg cttcgccatg cgggcgacga tgccggcctg
     3901 gcagcggtcg tcaagcaact cgacggatgg gcgagcgagg cggcgttcaa gccggtcttc
     3961 gagttctatg ccggcctgct ggcgcgcgac ggcgtgcgca acaggatgat cgagcggctc
     4021 ggactggaag ccggcgatat tctggacgaa ttcctgagct tctgccttgc cgaggagaga
     4081 accgggcttc ccggactgga aacattcctg gcgacgctgg aaaatgccgg ccccgaaatc
     4141 aagcgcgaga tggaccaggg ccgcgacgag gtgcgggtca tgacggtgca cgcggccaag
     4201 gggctggaag cgcccgtggt attcctggtc gatggcggtt cggcaccctt cagcgaccag
     4261 catctgccgc gcctgatgcc cttcgagggg cctggcgggg attggcgggg caagggctat
     4321 ctgtggcgcg ccgcaggcga cgtcgccaac gagttttcgc aaagtgcggc caaactggcg
     4381 cgcgatctgg ccgacgacga ataccgccgg ctgctctatg tcggcatgac ccgcgccgag
     4441 gaccggctgg tggtctgcgg ctatcacggc aagcgcgcgc cgaatgccgg cacatggcac
     4501 tccatcgtca gccgcgcgct ggtcggcgca ccgggcagca gcgagcgccg gcatccggtc
     4561 accgacgagc cgatctaccg gttccaggta acgaacctgc cgccggtttc gccggagccg
     4621 gtggaagcgg
//
LOCUS       DASYCS010000211         1608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4976277,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000211 DASYCS010000000
VERSION     DASYCS010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4976277"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VGM46_06820"
     CDS             <1..528
                     /locus_tag="VGM46_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019858171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY4192342.1"
                     /translation="DGEGYVFYEVSAVTPARDRTLDEVRQKVVADWTAAEAAKLLDDK
                     AQALEKRLKTGETLDVIASELKLEKQTKRGLKRDADDADLGKDGAAATFGVGEGGAGL
                     VAAPTGDAQILFKVVEVTEPAGADASTLPDDVQKSFASGISDDLLDQLVAHLQTQYKV
                     QVDQVAISQAQAQTR"
     gene            525..1535
                     /gene="trpD"
                     /locus_tag="VGM46_06825"
     CDS             525..1535
                     /gene="trpD"
                     /locus_tag="VGM46_06825"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019858172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEY4192343.1"
                     /translation="MSTLKAHIAKVASGSPLSFDEAREAFNIIMSGDATPGQIGGFLM
                     ALRVRGETVDEISGAVATMREKMLRVEAPADAIDIVGTGGDGSHSVNISTGSAFVIAA
                     GGVPVAKHGNRGLSSLTGTADVLTALGVKIDLPPEGISRCIAQAGLGFMFAPSHHPAM
                     KHVGPTRVELGTRTIFNILGPLSNPAGVTRQLVGVFSPEWVVPVAETLKALGSEHVWV
                     VHGDGYDEITTTGETQVAELTGGKISNFTLTPESVGLKRWNKQDLHGGDAGYNAEALR
                     AMLGGAPGAFRETVLMNAGAGLVVAGKVTTLADGMIAAAQAIDSGRALAVLDRLAKIS
                     NE"
BASE COUNT          313 a          517 c          529 g          249 t
ORIGIN      
        1 gatggcgagg gctacgtgtt ctacgaagta agcgccgtca cccctgcccg cgaccgcacg
       61 ctggacgaag ttcgccagaa agtcgttgcc gactggacgg cagcggaagc ggccaagctg
      121 ctcgacgaca aggcgcaggc gctggaaaag cggctgaaga ccggcgagac gctcgacgtc
      181 atcgccagcg agctgaagct cgaaaagcag accaagcgcg gcctgaagcg cgatgccgac
      241 gatgccgacc tcggcaagga cggcgccgca gcgacgttcg gcgtcggtga aggcggcgct
      301 ggcctggtcg ccgcgcccac cggcgatgcg caaatcctgt tcaaggtcgt ggaagtgacc
      361 gagccggcgg gcgccgatgc cagcaccttg ccggacgatg tgcaaaaatc cttcgcctcc
      421 ggcatttctg acgatttgct cgaccaattg gtcgcccacc tgcaaacgca gtacaaggtg
      481 caggtcgacc aggttgccat ttcacaggct caagcacaga cgcgatgagt acgctgaaag
      541 cccatatcgc caaggtggcc tccggcagcc cgctttcttt cgacgaggcg cgcgaggctt
      601 tcaacatcat catgtcgggc gatgcgacgc ccggccagat cggcggcttc ctgatggcgc
      661 tgcgcgtgcg cggcgagacg gtcgacgaga tttccggcgc ggtcgccacc atgcgtgaaa
      721 agatgctgcg cgtcgaagcg ccggccgacg ctatcgacat tgtcggcacc ggcggcgatg
      781 gctcgcacag cgtcaacatc tcgaccggct cggctttcgt catcgcggcc ggcggcgtgc
      841 ccgtcgccaa gcacggaaat cgcgggcttt catcgctgac cggcacggcg gacgtgctga
      901 cggcgcttgg cgtcaagatc gacctgccgc cggaaggcat cagccgctgc atcgcgcagg
      961 ccggccttgg cttcatgttc gcgccatccc atcatccggc catgaagcat gtcgggccga
     1021 cccgcgtgga actcggcacc cgcaccatct tcaacatact cggaccgctt tccaatccgg
     1081 caggcgtcac ccggcaactg gtcggcgtgt tctcgcccga atgggtagtg cctgtcgccg
     1141 aaacgctgaa ggcgctcggc tccgagcatg tctgggtcgt tcatggcgac ggctatgacg
     1201 agatcaccac gaccggcgaa acgcaggtgg cggaactgac cggcggaaag atcagcaact
     1261 tcaccctgac acccgaatcc gtcggcctga aacgctggaa caagcaggat ttgcacggcg
     1321 gcgacgccgg atataatgcc gaggcgttgc gcgccatgct tggcggcgcg ccgggcgctt
     1381 tccgcgaaac cgtgctgatg aacgcgggag cgggcctggt tgttgcgggc aaggtgacga
     1441 cgctggccga tggcatgatt gcggcggcgc aagccatcga cagcggccgc gcgctggccg
     1501 tgctggacag actggcaaag atttcgaacg agtaacgcca tggccgacat attgcgcaag
     1561 atcgaagcct acaagcgcga cgagatcgcc gccgccaaag cgcgcgcg
//
LOCUS       DASYCS010000212         3016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_485581,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000212 DASYCS010000000
VERSION     DASYCS010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485581"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VGM46_06830"
     CDS             <1..221
                     /locus_tag="VGM46_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEY4192344.1"
                     /translation="KSLKIPGDRMLFVILAVALYILLLVSYPWYTLTASVAVYLVFLP
                     FSMRAYSRRALREGDASLRPDVDERSPE"
     gene            complement(237..2459)
                     /locus_tag="VGM46_06835"
     CDS             complement(237..2459)
                     /locus_tag="VGM46_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018427092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY4192345.1"
                     /translation="MILTLSLLAGFALLWLLVGVICKASLGLGMAQAMLYVPFKLAFR
                     IDDSRMKIARQAKTPVIYVVTHQARLDPALMLSLLPPETLHILDDASAKSPWLEPWRE
                     LARTIAFNAEHVFVSRRLVRVLKGNGRLAVYLPNEVEPDIKSFRLYRAVARIALQADA
                     SIVPIFVGGARHLPGSLTPAANAPRRWFARLSISVLAPMTITELARRAGETWTTNSNA
                     LFDRVAEARLAATDLSRGLFNAMRDASDRIGHSHPIIADVVSGQMSYRQMFIAARVLG
                     RRFEEKTAPGEVVGLLLPNANGVVLSLTGLWSSGRTAALVNYTAGPASITSAIRTAVV
                     RTVISSRAFIEKAGLADEVEAVEKGGAKFLWLEDIRQSVTRLEKLTAALLWRYPLQRQ
                     DGDKPAVVLFTSGSEGTPKAVVLSHKNLLANAMQAEARITISPADTLLNVLPVFHSFG
                     LTGGTILPLVTGVKLFLYPSPLHYKLIPEIARKVRPTVMFGTDTFLANYARAAKDGDF
                     SSLRFVVAGAEAVKAETRRIYRERFQAEIVEGFGLTEAAPVVAVNTAIHGRDGTVGRL
                     LPGMRMRLEPVEGVADAGRLWISGPNLMMGTMSAERPGELQPPEDGWHDTGDIVSVDR
                     EGFIAIRGRAKRFAKVAGEMVSLGAVEMLVQSLWPEEHHAAVAIPDKKRGERIVLVTT
                     ADAADPETLRRAGKQAGVGELMVPHDIVKVAEIPVLGSGKTDYTSARKLALDRLVERI
                     "
     gene            complement(2716..>3016)
                     /locus_tag="VGM46_06840"
     CDS             complement(2716..>3016)
                     /locus_tag="VGM46_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019857250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY4192346.1"
                     /translation="VTDPLAVSPRDAVLLVAFGISFAVAAILWTEGARLIPAAESGLL
                     GSAEVPFAILFAFLFLSEIPPAASMLGGAIVILAVFAHAGRDWLAARAITQSPDG"
BASE COUNT          583 a          972 c          936 g          525 t
ORIGIN      
        1 gcaagagcct gaaaattccc ggcgaccgga tgcttttcgt catccttgcc gtcgcgctct
       61 atatcctgct gctggtcagc tatccctggt acacgctgac cgcttcggtc gccgtctatc
      121 tggttttcct gccgttcagc atgcgggcct attcccgccg cgccttgcgc gagggcgatg
      181 cgtctctgcg gccggatgtc gacgagcggt ctcccgaata gcgctggcct acgggctcaa
      241 attcgctcca ccagcctgtc aagcgccagc ttgcgtgctg acgtgtagtc cgtcttgccg
      301 gaacccagca ccgggatttc cgccactttg acgatatcgt gcggcaccat cagttcgccg
      361 acgccggcct gcttgcctgc ccggcgcagg gtttcagggt cggcagcgtc cgccgtggtg
      421 acgagcacga tgcgttcgcc gcgcttcttg tccggtatcg ccaccgctgc atggtgttct
      481 tccggccaga gagactgcac cagcatttcg acggcgccca gcgacaccat ctcgcccgca
      541 accttggcga aacgcttggc ccggccgcga atggcgatga agccctcgcg gtcgacggaa
      601 acgatgtcgc cggtatcgtg ccagccatct tccggcggct ggagttcgcc cggccgctca
      661 gccgacatgg tgcccatcat caggttcggc ccggaaatcc atagccgtcc ggcatccgca
      721 acgccctcga cgggttccag ccgcatccgc attcccggca acagccgtcc gacagtgccg
      781 tcgcggccat ggatcgcggt gttgacggca acgaccgggg cggcttccgt caggccgaaa
      841 ccctcgacga tttcggcctg gaagcgctcg cgatagatgc gccgcgtctc ggctttgacc
      901 gcctccgcgc cggcaacgac gaagcgcagg ctggagaaat cgccatcctt ggcggcgcgc
      961 gcataattgg cgaggaacgt atcggtgccg aacatgacgg tcgggcgcac cttgcgcgca
     1021 atttcgggga tgagtttgta atgcaggggc gagggataga ggaaaagctt taccccggtg
     1081 accagcggca aaatggtgcc gcccgtcagc ccgaacgagt gaaacaccgg tagtacgttg
     1141 agcagtgtgt cggccggaga aatcgtgatg cgcgcctcgg cctgcatggc attggccaaa
     1201 aggttcttgt gcgaaagcac cacagccttg ggcgtgcctt ccgaaccgga ggtgaacagc
     1261 acgaccgccg gtttgtcgcc atcctgcctt tgcagcgggt aacgccacaa aagtgctgcc
     1321 gtcagttttt ccagcctggt cacgctctgg cggatatcct ccagccagag gaacttggcg
     1381 ccgccttttt ccaccgcctc cacctcatcg gcaaggccgg ctttttcgat gaacgcgcgc
     1441 gaagaaatga cggtgcgcac aaccgccgtt cgtatcgcgg aggtgatgct cgccggtccg
     1501 gccgtataat tgaccagcgc tgcggttcgg cccgacgacc acagcccggt cagcgacaaa
     1561 acaacgccat tggcattggg caaaagcagc ccgaccactt cgccgggcgc ggtcttttcc
     1621 tcgaagcgcc ggccaagtac gcgcgcggca atgaacattt gccggtagct catctggccg
     1681 gaaaccacat ccgcgatgat cggatgcgaa tgaccgatgc ggtcggaggc gtcgcgcatg
     1741 gcgttgaaca acccgcgcga aagatccgtc gccgccagcc gggcctcggc gacacggtcg
     1801 aacagcgcat tgctgttggt cgtccaggtc tcgccggcgc gccgggccag ttccgtaatc
     1861 gtcatcggcg ccaatacgct gatggaaaga cgggcaaacc agcgtcttgg cgcgttggcg
     1921 gcaggcgtca gcgaaccggg aagatggcgc gcgccgccaa cgaatatcgg aacgatgctc
     1981 gcatccgcct gaagggcgat gcgcgcgacg gcgcggtaaa gccggaacga cttgatatcc
     2041 ggctccactt cgttcggcag atagacggca agccggccat tgcccttcaa cacgcgcacc
     2101 agccggcgac tgacgaagac atgctcggcg ttgaacgcga tggtgcgcgc cagttcgcgc
     2161 catggttcca gccagggcga tttggccgaa gcatcgtcca gaatatgcag ggtttcaggc
     2221 ggcagaagcg acagcatcag cgccgggtcg agcctcgcct ggtgcgtcac cacatagatc
     2281 accggcgtct tcgcctgccg cgcgatcttc atgcggctgt cgtcgatgcg aaaggccagc
     2341 ttgaacggta cgtagagcat ggcctgcgcc atgcccaacc ccaggcttgc cttgcagatc
     2401 acgccgacca gcagccagag cagggcgaag ccggccagca gcgaaagggt caggatcatg
     2461 ccgcaccact cccaacggtt cgcaccacgg cctttcggcc tgcgtcggca acgaaactac
     2521 tgcatcgatc cgcaaatcga taccgatttt cgtcaggcgc gatatgggaa ttagacaaga
     2581 ctctgttttt catgaagaaa accgccattc ggctggcgtc gagaagaaca gaccaagtgt
     2641 ggagggactg gaggggcggc tcgcaaccca catgatgccc cgtggttaca aagcgtcccg
     2701 tccgcccgga ctttttcatc cgtccgggct ttgggtgatt gcccttgccg ccagccagtc
     2761 gcggccggca tgggcgaata cggcgaggat cacgatggcg ccgcccagca tactggcggc
     2821 cggtggaatc tcggacagga agaggaaggc gaaaagaatg gcgaagggta cttccgccga
     2881 gccgagcagg cccgattccg ccgccggaat aagccgcgcg ccttccgtcc acaaaatggc
     2941 ggcgacggca aatgaaatgc cgaaggccac cagcaacacg gcgtcgcgag gcgacaccgc
     3001 aagaggatcg gtaacc
//
LOCUS       DASYCS010000213         1868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_774383,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000213 DASYCS010000000
VERSION     DASYCS010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774383"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..966)
                     /locus_tag="VGM46_06845"
     CDS             complement(40..966)
                     /locus_tag="VGM46_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003494114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HEY4192347.1"
                     /translation="MSADFAAVDWGTSSFRLWLMQADGAVLAQSRGPEGMQHCHGVGF
                     APVLASHLAKAGAPAGLPVLICGMAGARQGWAEVAYADLPALVADIAANALKLPGALL
                     QGLGEERDVRILPGLAQNDPARPDVMRGEETQLLGLAQMGHQGLACIPGTHSKWAMLE
                     DGAVTSMRSAMTGELFDILSKQSILRHGMPLQGRVSADDAAFLNAVDEMLADPAAALT
                     SLFPLRAAGLLGFATPEAVQARLSGLLIGAEIGKMLAGHECGPIMLIGQEGLGALYRA
                     ALLRAGVSLRYADAEEASRQGLLAAARKIWSF"
     gene            complement(963..1745)
                     /locus_tag="VGM46_06850"
     CDS             complement(963..1745)
                     /locus_tag="VGM46_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018067390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY4192348.1"
                     /translation="MTSTRLRGRFIAVTGAGQGIGQAIAEDFSAEGAGLFLIDRDEDN
                     LRSVAAQLSERGGHVVFAVVDITDASGIARAFADAATTAGRAFDGLVNNAGVNVFHAP
                     LETSDEEWRRAFAVNLEGAWSCARAVLPGMIARRHGVILNIASTHSFTIIPHTFPYPV
                     VKHALLGLTKSLALEHAADGVRVNAIAPGYVATQKVIDWWNDHPDPAAAKAETLALHP
                     QGRIATPQEIARAAVFMISDECPFMTATSLTIDGGLSVRQHA"
     gene            complement(1747..>1868)
                     /locus_tag="VGM46_06855"
     CDS             complement(1747..>1868)
                     /locus_tag="VGM46_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018497475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-galactonate dehydratase"
                     /protein_id="HEY4192349.1"
                     /translation="EGPGLGIEINEEAVIEAAKIGHRWRNPLWRHQDGSVAEW"
BASE COUNT          335 a          624 c          589 g          320 t
ORIGIN      
        1 ggccgcgttt cagttgcggc cagaccacct tgtttgtcat caaaaactcc agatttttct
       61 tgctgcggcc agcagaccct gccggctggc ttcttcggca tcggcatagc gcagcgacac
      121 gccggcgcgc agaagcgctg cacgatagag tgcgccaagc ccttcctgcc cgatcagcat
      181 gatcgggccg cattcatgac cggcaagcat cttgccgatt tccgcaccga tcagcaggcc
      241 ggaaagacgc gcctgcaccg cctccggcgt agcgaagccg agcaaaccgg cagcgcgcag
      301 cggaaagaga gaggtcagcg ccgccgccgg atcggcaagc atttcgtcca ctgcgttcag
      361 gaaggcggca tcgtccgccg atacgcggcc ttgcaaaggc atgccgtgcc gaaggatgct
      421 ctgcttcgac aatatgtcga acagctcacc ggtcatcgcg ctgcgcatcg aggtgacagc
      481 gccgtcttcg agcatggccc atttggaatg ggtgcccggt atgcaggcca gtccctggtg
      541 ccccatctgc gcgagaccca ggagttgcgt ttcctcgccg cgcatcacgt ccggccgcgc
      601 cggatcgttc tgcgccagac cgggcaggat tcgaacgtcg cgttcctccc caagcccctg
      661 aagaagcgcg ccgggcagtt tcagggcatt tgcggcaatg tcggcgacca gcgctggaag
      721 gtccgcataa gcgacctcgg cccaaccctg ccgcgcgccg gccattccgc atatgagaac
      781 gggcaggcct gccggagcac cggccttggc cagatgcgac gccagcaccg gcgcaaagcc
      841 cacgccatgg caatgctgca tcccttccgg gccgcgggac tgagccagga cggcgccgtc
      901 cgcctgcatc agccaaaggc ggaaactgga ggtgccccaa tcaacggcag cgaaatcggc
      961 gctcatgcgt gctgcctcac gctcagcccg ccatcgatgg tcaaggaggt cgcggtcatg
     1021 aacgggcatt cgtcggaaat catgaatacg gcggcccgcg caatctcctg cggcgtggcg
     1081 atccggcctt gcgggtgaag cgccagagtc tcggccttcg ccgccgccgg atcgggatga
     1141 tcgttccacc agtcgatgac cttttgcgtc gcgacatagc cgggcgcgat ggcattcacc
     1201 cgcacgccgt ccgccgcatg ttccagcgcc agcgacttgg tgaggccgag aagcgcgtgc
     1261 ttcaccaccg gataggggaa ggtatggggg atgatggtga aggaatgggt ggatgcgata
     1321 ttgaggataa cgccgtgccg gcgcgcgatc atgcccggaa gcaccgcgcg cgcgcagctc
     1381 catgcacctt cgagattgac cgcgaaggcg cgccgccatt cctcgtcgct cgtctctaaa
     1441 ggggcatgaa agacgttcac ccccgcattg ttgacgagac cgtcgaaggc acggccggcg
     1501 gtggtggcgg catcggcgaa agccctggct atgccggagg catcggtgat gtcgacgacg
     1561 gcgaacacga cgtggccgcc gcgttcggac aattgcgcgg cgacgctgcg cagattgtcc
     1621 tcgtcgcggt cgatcaggaa gaggccagcg ccttccgcgc tgaaatcctc ggcgatggcc
     1681 tggcctattc cctggccggc acccgtcacc gcgatgaacc gtccccgcag tcttgtgctc
     1741 gtcatgtcac cactccgcga cgcttccatc ctgatggcgc cacagtgggt tgcgccagcg
     1801 atggccgatt ttcgccgctt cgatcaccgc ttcctcgttt atttctatac cgaggcccgg
     1861 cccttcgg
//
LOCUS       DASYCS010000214         6807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4976665,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000214 DASYCS010000000
VERSION     DASYCS010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 6807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6807
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4976665"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VGM46_06860"
     CDS             complement(<1..435)
                     /locus_tag="VGM46_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY4192350.1"
                     /translation="MNLIYALLALLMALVFTLVGITRAGIWLIERANPPVGNFADIND
                     ASIHYVHVPAPIGAELPPIVFIHGASANLKDQMLPLRPLLEGRAEMLFFDRPGHGWSG
                     RGRGNNGSPAAQADTIAALMDHLGMKKAIIVGHSFGGALAAAF"
     gene            complement(515..1099)
                     /locus_tag="VGM46_06865"
     CDS             complement(515..1099)
                     /locus_tag="VGM46_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192351.1"
                     /translation="MRNVFAFLILLSCAVGFAQPAWADDAIAATAPLDRKARLDRLFV
                     DLKHERNDKAAERIAGNIWQEWSDSGSASINLMMQWSQKAIEAQKFDVALDFLDQVTT
                     LDPTYAEGWNRRATVHFMMKSYGKSMADIGQALELEPRHFGALSGMAQIMADTGHKQS
                     ALAAWQRVLALYPMMRSAQNEVSTLSEELAGEGI"
     gene            complement(1208..1333)
                     /gene="ykgO"
                     /locus_tag="VGM46_06870"
     CDS             complement(1208..1333)
                     /gene="ykgO"
                     /locus_tag="VGM46_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012177066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HEY4192352.1"
                     /translation="MKIKNSLKSLRARHRDNQLVRRKGRVYIINKTAPRFKARQG"
     gene            complement(1484..2569)
                     /locus_tag="VGM46_06875"
     CDS             complement(1484..2569)
                     /locus_tag="VGM46_06875"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEY4192353.1"
                     /translation="MSLPAGSTIGIIGGGQLGRMLAMAAARLGYRTVVLEPQPDCPAA
                     QVANRQIIAAYDDPAALAELANASAIVTYEFENVPVEAAEALTAKVPVYPPARALEVA
                     QDRVAEKTFLNGIGVTTADFRPVANDDELTEALAKFDGSGVLKTRRMGYDGKGQRVFR
                     NMTAGGFAGTCEAMGSVPLILEALVDFEREISVIAARGVDGSIAAFDPAENVHRQGIL
                     HTSTVPAAISAATAAAAQSAAKAILAALDYVGVIGVEFFVLADGSLLANEIAPRVHNS
                     GHWTEAACIVSQFEQHIRAIAGLPLGRPDRHSDCVMENLIGDDVSRMGALLAEPDLML
                     HLYGKAEARPGRKMGHFTRTTPRIARN"
     gene            complement(2566..3063)
                     /gene="purE"
                     /locus_tag="VGM46_06880"
     CDS             complement(2566..3063)
                     /gene="purE"
                     /locus_tag="VGM46_06880"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEY4192354.1"
                     /translation="MSDQRVEIAIIMGSQSDWATMRHAAETLEALDLPHKALIVSAHR
                     TPDRLYDFAKGAKASGYKVIIAGAGGAAHLPGMTAAMTPLPVFGVPVESKALSGQDSL
                     LSIVQMPAGIPVGTLAIGKAGAVNAALLAAAVLALSDEPLAARLETWREAQTAKVAIE
                     PVDAP"
     gene            complement(3152..3355)
                     /locus_tag="VGM46_06885"
     CDS             complement(3152..3355)
                     /locus_tag="VGM46_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HEY4192355.1"
                     /translation="MSEQEQADIRLEFSRLKQDHADFDAAINAMIAMACDPLQIQRMK
                     KKKLFIKDRLMALEDRIIPDIIA"
     gene            3594..3773
                     /locus_tag="VGM46_06890"
     CDS             3594..3773
                     /locus_tag="VGM46_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HEY4192356.1"
                     /translation="MSLANHLDELQRKHGDIERALDSAMNHPSVDDLEIVDLKRRKLA
                     LKDEIEKLKAPPRAH"
     gene            complement(4091..4231)
                     /locus_tag="VGM46_06895"
     CDS             complement(4091..4231)
                     /locus_tag="VGM46_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HEY4192357.1"
                     /translation="MNPIRTFRNWRMYKETVRELNRLNTRQLSDLGINRSEIEQIART
                     YR"
     gene            complement(4372..5376)
                     /locus_tag="VGM46_06900"
     CDS             complement(4372..5376)
                     /locus_tag="VGM46_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002967904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEY4192358.1"
                     /translation="MPEKMPEKMNTVAISAPGGPMVLKPERRAVPQPGPGEILIRVHA
                     AGVNRPDVLQRKGAYPPPKGASDLPGLEVAGEVAALGEGAARWRVGDRVCALTAGGGY
                     AEYVTVHASNVLPLPHGFTYTEAAALPETYFTVWHNVFERGALKSGEVLLVHGGSSGI
                     GTTAIQLASAFGATVIATAGSAEKCAACLKLGAARAINYREEDFVAAVKETTNGAGAN
                     VILDMVGGDYVGRNYEAAAVEGRIVQIATQGGAVASTDFSRLMIKRLVHTGSTLRPRT
                     VEFKGKVAAALESQVWPLLATRRVAPVMDMIFPLNDAWRAHERMEEGDHIGKIVLDVG
                     "
     gene            5496..5690
                     /locus_tag="VGM46_06905"
     CDS             5496..5690
                     /locus_tag="VGM46_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1192 domain-containing protein"
                     /protein_id="HEY4192359.1"
                     /translation="MAIFDDEPQKKPKPHEIGQDLSLLSQGELAERIGLLRGEIARLE
                     AELLAKGTTKAAAEALFRRG"
     gene            5764..>6807
                     /locus_tag="VGM46_06910"
     CDS             5764..>6807
                     /locus_tag="VGM46_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4192360.1"
                     /translation="MFATYRPIFSLLRGTAFLLAASGLHGLLLPLRGQAEGFSTASLG
                     LMGTAWAGGFVTGCFFAPRIVRRAGHVRAFGTFAASGAIVALLTGLIIDEYVWILLRA
                     CTGFVMAGCFMVIESWLNEKATNESRGTIFGMYMMVTYASIMAGQMIVAAGDVRSASL
                     FMITGIFFCLSLIPTAVSSASHPKPLQDVSLDLKELYANSPVSAVGCLLVGIANGAWG
                     TLGAVYGARIGVSTAQIALMMSIVVVAGAAMQLPAGRLSDKTDRRYVLAGAAFGAAFF
                     AILLFLFQPRSATFVLVFTGAYGAFAYTLYSIAVAHANDHADAEDFVKVSSGLLMLYG
                     FGTMIGPLLAAGLM"
BASE COUNT         1177 a         2043 c         2269 g         1318 t
ORIGIN      
        1 gaaggcggcg gccagtgcgc cgccgaagga atggccgacg atgatcgcct tcttcatgcc
       61 gagatggtcc atgagcgcgg cgatggtgtc ggcttgcgct gccgggctgc cattattgcc
      121 acgaccgcgc ccggaccagc catggccggg gcggtcgaag aacagcattt cggcgcggcc
      181 ttcgagcagc gggcgaagcg gcagcatctg gtccttgagg ttggcgctgg cgccatggat
      241 gaaaacaatc ggcggcagtt ccgcccctat gggcgccggc acatggacat agtggatgct
      301 ggcgtcgttt atgtcggcga aattcccgac cggcgggttg gcgcgctcga tcagccagat
      361 gccagcgcgg gtaatgccca ccagcgtaaa gacaagcgcc atcagcaagg cgagcagggc
      421 atagatcagg ttcatagggg acaggtacga ggcaggaggg aaatcggttc agttcaaatg
      481 agttcggaca aaggacatca tcatgccaga cgggtcagat gccttcgccg gccagttcct
      541 cggaaagcgt cgacacctcg ttttgggcgc tgcgcatcat cgggtagagg gccagcacgc
      601 gctgccacgc cgccagcgcc gactgcttgt ggccggtgtc ggccatgatc tgcgccatgc
      661 cggaaagcgc gccgaaatgg cgcggctcca gttccagcgc ctgtccgatg tcggccatcg
      721 acttgccgta gcttttcatc atgaagtgca cggtggcgcg gcggttccag ccttcggcat
      781 aggtggggtc gagcgtcgtc acctggtcga ggaaatcgag cgcgacgtcg aatttctgcg
      841 cttcgatggc tttttgcgac cattgcatca tcaaattgat ggaagcgctg ccggaatcgg
      901 accattcctg ccagatattg ccggcgatgc gctcggcagc cttgtcgttg cgctcatgct
      961 tgagatcgac gaacaaccgg tcgagacgcg ccttgcggtc gagcggggcg gtggcggcga
     1021 tcgcatcatc ggcccaggca ggctgcgcga agccaacggc acatgaaaga aggatcagaa
     1081 acgcaaaaac attccgcatg gccgggaatc taaccccgac cggcggaacg tcaaactgaa
     1141 tttagcgtga cagccggcca agccggcagc ggggctaaag ccgaaggctc aagccctcgg
     1201 caaaaactca accctggcgc gccttgaagc gcggagcggt cttgttgatg atgtagacgc
     1261 ggcccttgcg gcgaaccaac tggttgtcgc ggtgacgggc gcggagcgac ttgagcgaat
     1321 tcttgatctt catgttcttg cctgttggtc gatgcccgct gccgggctga attctcgcgg
     1381 cacgcccgaa gacacgccat tctggtgccc ggctcataga cgaggagtct cgtccatgtc
     1441 aaccaccggg cctcgccgaa agggccgatt gagcgcctga acttcagttg cgggcgatgc
     1501 ggggcgtggt tcgcgtgaaa tgccccatct tgcggcccgg ccgcgcctcg gccttgccgt
     1561 agagatgcag catcagatcc ggctcggcca gcagcgcgcc catgcgcgaa acatcgtcgc
     1621 cgatcaagtt ttccatcacg cagtccgaat gccggtcggg ccgcccgagc ggcaggccgg
     1681 caatggcgcg gatatgctgt tcgaattgcg agacgatgca ggcggcttcc gtccagtggc
     1741 cggaattgtg aacgcgcggc gcgatctcgt tggcgagcag cgaaccatcg gccagcacga
     1801 agaattcgac gccgatgacg ccgacatagt cgagcgcggc aaggatggcc tttgcggcag
     1861 actgcgcggc ggcggctgtg gcggccgaaa tcgccgcggg cacggtcgag gtgtgaagga
     1921 tgccctggcg gtgcacgttt tcggccgggt cgaaggcggc aatcgatccg tcgacgccgc
     1981 gcgcggcgat caccgatatt tcgcgctcga aatcgaccag cgcttccagg atgagcggca
     2041 ccgaacccat cgcctcgcag gttcccgcga aaccgccggc ggtcatgttg cggaagacgc
     2101 gctggccctt gccgtcatag cccatgcggc gcgtcttcaa tacgccgctg ccgtcgaatt
     2161 tcgccagcgc ctcggtcagt tcgtcgtcat tggcgacggg gcggaaatcg gcggtggtga
     2221 cgccgatgcc gttcaggaac gtcttttccg ccacccggtc ctgcgccact tcgagcgcgc
     2281 gggcgggcgg atagactggc acttttgccg tcagtgcctc tgccgcttcc acgggtacgt
     2341 tttcgaactc gtaggtgacg atcgcggatg cgttggcgag ttcggcaagg gcggcgggat
     2401 cgtcataggc ggcgatgatc tgccggttgg cgacctgtgc ggcggggcaa tccggctgcg
     2461 gttccagcac gacggtgcgg tagccgaggc gggcggcggc catcgccagc atgcggccaa
     2521 gctggccgcc gccgatgatg ccgatggtgg atccggcggg caggctcatg gcgcgtccac
     2581 cggctcgatg gcgaccttgg cggtctgggc ttcacgccag gtttcaaggc gcgcggccag
     2641 cggctcatcg ctgagcgcca gaacggcagc cgccaacagg gcggcgttga ccgcgccagc
     2701 cttgccgatg gccagcgtgc cgacggggat gccggccggc atctggacga tggaaagcag
     2761 cgaatcctgc ccggacaggg ccttggattc gaccggcacg ccgaaaaccg gcagcggcgt
     2821 catcgcggcg gtcatgccgg gcagatgggc cgcgccgccg gcgccggcga tgattacctt
     2881 gtagccggac gccttggcgc ccttggcgaa gtcataaagc ctgtcgggag tgcggtgggc
     2941 ggaaacgatc agcgccttat ggggcaaatc cagcgcttcg agcgtttcgg cggcgtggcg
     3001 catcgttgcc caatcggact ggctgcccat gatgatggcg atttcgacgc gttgatcgct
     3061 caatgtcagg ctcccggcga taaagcgacg gcgtagccga aattacccga acgggcaagg
     3121 atcggcggcc gcgcgcagcc cgtggttggc tctaggcgat gatgtcggga atgatccggt
     3181 cttccagcgc catcagccga tccttgatga aaagcttctt cttcttcatg cgctggattt
     3241 gcagcgggtc gcaggccatg gcgatcatgg cgttgatggc cgcgtcgaaa tcggcatggt
     3301 cctgcttcaa tcgcgaaaat tcgaggcgaa tatcggcctg ttcctgttcg gacatggttc
     3361 cgtctgtatg tctgcggcgc cctgcaacgg gctgtcgggg cgggaccggc atcgaaaagc
     3421 aatgccggcg aaatttcttt agcacgtttc actttgtcgg gaaatgcatg agtgcgcatt
     3481 cgacattgcc gcatgatggt ggcagactgt catcgtgccg tcacagaagc aaaactgcgg
     3541 tggaactgtc ttggcggctg tagtcgagga aaccatgaaa gggagtacca atcatgtctc
     3601 ttgccaacca tcttgacgag ttgcagagga agcatggcga tatcgagcgt gcgctcgaca
     3661 gcgcgatgaa ccatccctcg gtcgacgatc ttgaaatcgt cgatctcaag cgacgcaagc
     3721 tggccctcaa ggacgagatt gagaaactca aggcgccgcc aagggcgcac tgatcgggag
     3781 ccgctaccac ccggaatcga caggcaggcc gcgctggctc cagcgttcgg cgccggcgaa
     3841 atgccatctg ctttgacgca atggtttgcc gttgcgcagc gctggcgcgg cgtcgttatg
     3901 gcggcgcttt agtggcgcgg ctgagccgat tttatgccgg caggccggtc gatttatgat
     3961 ccggacaaaa acaaacccgc cggacaaagt ctggcgggtt cggagccggc tgcggaaaac
     4021 cgcgcgttcg gctctggctt tgctgcttgt ccacgccgcg caaaaatgcg cgttgcggag
     4081 ccgcgccgcc ttagcggtag gtgcgggcga tctgctcgat ttcagagcgg ttgatgccga
     4141 gatcgctaag ctggcgggtg ttgagacggt tcagttcgcg cacggtctct ttatacatgc
     4201 gccagttacg gaaagtgcgg atcgggttca tggtttcctc catcggggtt gttcgatctg
     4261 atggagggta gataattcct tcggcgcgcg aaataaatgg atgcttttgc atggttgcaa
     4321 tgcaaatgct gcattgcaac attaatgcaa agttaagcgg caaagcaccg ttcagccgac
     4381 atccagcacg atcttgccga tgtgatcgcc ctcctccatc cgctcatgcg cgcgccaggc
     4441 gtcgttgagc gggaagatca tgtccatgac cggcgccacg cggcgggtcg ccagcagcgg
     4501 ccagacctgg ctttccagcg cagcggcgac cttgcccttg aactccaccg ttcgcggacg
     4561 cagcgtggaa ccggtatgga ccagccgctt gatcatcagc cgcgagaagt cggtgcttgc
     4621 caccgccccg ccctgggtgg cgatctggac gatgcggcct tcgacggcgg ctgcctcata
     4681 gttgcggccg acatagtcgc cgccgaccat atcgagaatg acattggcgc cagcgccatt
     4741 cgtcgtctcc ttgacggcgg cgacgaaatc ctcctcgcgg taattgattg cgcgggcagc
     4801 gccgagcttg aggcaggcgg cgcatttctc cgccgagccc gctgtggcga tgacggtggc
     4861 gccgaaggcc gaggcgagct ggatcgccgt agtgccgatg cccgacgagc cgccatgcac
     4921 cagaaggacc tcgccgcttt tcagggcgcc gcgttcaaac acattgtgcc agacggtgaa
     4981 gtaggtttcg ggcagcgcgg cggcttccgt gtaggtgaag ccgtgcggca gcggcaagac
     5041 attgctggca tggacggtga catattcggc gtagccgccg ccggccgtca gcgcgcaaac
     5101 gcggtcgccg acgcgccagc gggcagcgcc ttcgccaagt gcggccactt cgccggccac
     5161 ttcgaggcct ggcaggtcgg acgcgccctt gggtggcggg taggcgccct tgcgctgcaa
     5221 cacgtcgggc cggttgacgc cggcggcatg aacgcggatc aggatttcgc cggggccggg
     5281 ctggggcacg gcgcggcgct ccggtttcaa caccatcggg ccgcccggcg cggaaatcgc
     5341 caccgtattc atcttttccg gcatcttttc ggggatcgcg tgttctgcgt cggtcattat
     5401 ccgtccagtc cagtttgttt ttgatcgctg tcgttgctct atgtatgctc attggcaaat
     5461 caggcaaact gttgcggtca gcacaaggca cgacgatggc gatcttcgac gacgagccgc
     5521 agaaaaagcc gaagccgcac gagatcgggc aggatttgtc gctgctgtcg cagggtgagc
     5581 ttgccgagcg gataggcttg ctgcgcggcg agattgcaag gctggaggcc gagcttctgg
     5641 caaaaggcac gaccaaggcg gcggctgaag cgttgttccg gcgcgggtga atgcgatgct
     5701 tcgaaaagcc cgaggctacc ttgatccttc tttgaaaacg cggttctgat ccgaggcagt
     5761 tcgatgtttg cgacctacag gccgatcttc tccctgctgc gcggcaccgc gttcctgctg
     5821 gcggcatcgg ggttgcatgg gctgcttttg ccgctgcgcg gccaagccga gggcttttcc
     5881 acggcatcgc tcggcctgat gggcacggca tgggcgggcg gcttcgttac aggctgtttt
     5941 tttgcgccgc gcatcgtgcg ccgcgcgggc catgtgcggg ctttcggcac tttcgccgct
     6001 tcgggcgcca tcgtcgccct gctgaccggc ctcatcatcg acgaatatgt ctggatattg
     6061 ctgcgcgcct gcaccggctt cgtcatggcc ggctgcttca tggtcatcga aagctggctc
     6121 aacgagaagg cgacgaacga aagccggggc acgatcttcg gcatgtacat gatggtgacc
     6181 tacgcctcga tcatggccgg ccagatgatc gttgccgccg gcgacgtgcg ctcggcttcg
     6241 ctgttcatga ttaccggcat cttcttttgc ctgtcgctga tcccgaccgc cgtttcgagc
     6301 gcctcgcacc ccaagccgtt gcaggatgtt tcgctggacc tgaaggagct ttacgccaat
     6361 tctcccgtct cggcggtggg ttgcctgctg gtcggcatcg ccaatggcgc gtggggcacg
     6421 ctgggcgcgg tctatggcgc gcgcatcggc gtttcgacag cgcagatcgc cttgatgatg
     6481 tcgatcgtcg tcgtcgcggg cgctgccatg caactgcccg ccgggcgcct ttccgacaag
     6541 accgaccgcc gctacgtgct ggcgggcgcg gccttcgggg cggcgttttt tgcgatcctg
     6601 ctgtttttgt tccagccgcg ctccgccacg ttcgtgctgg tgtttacggg ggcctacggg
     6661 gcgttcgcct atacgctcta ttcgatcgcg gtggcccacg ccaacgacca cgccgacgcc
     6721 gaggatttcg tcaaagtgtc gagcgggctg ttgatgctct atggctttgg taccatgatc
     6781 ggcccgctgc tcgccgccgg cttgatg
//
LOCUS       DASYCS010000215         5016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_50140, whole
            genome shotgun sequence.
ACCESSION   DASYCS010000215 DASYCS010000000
VERSION     DASYCS010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 5016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5016
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50140"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..1153)
                     /locus_tag="VGM46_06915"
     CDS             complement(2..1153)
                     /locus_tag="VGM46_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008596158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4192361.1"
                     /translation="MSDIQLKSAVEQAREPVARPWLSPLNQRRWQSFKANRRGYWSLW
                     IFLLLFVLSLFSEFIANDKPIIASYKGEILFPVLVDYPEEKFGGFYAVADYRDPVIHD
                     EIEANGWMVWPPIRYSYTTVNNAIPEAAPAKPSWEYDAKARCAQYPKGVDDPDCNIGN
                     WNWLGTDDQARDVLARAIYGFRVSVLFGLILTIGSAVIGVSAGAVQGYFGGWIDLLFQ
                     RFIEIWSAIPVLYLLLIVAAILPPGFFILLGIMLLFSWVAFVGVVRAEFLRARNFEYV
                     NAARALGVPNRTIMFRHLLPNAMVATLTFLPFILNGSISTLTSLDFLGFGLPPGSASL
                     GELLKQGQRNLNAPWLGITGFVTLSIMLSLLIFVGEATRDAFDPRKTFK"
     gene            complement(1153..2259)
                     /locus_tag="VGM46_06920"
     CDS             complement(1153..2259)
                     /locus_tag="VGM46_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HEY4192362.1"
                     /translation="MGAYILRRILLMIPTLFGIMAISFAVIQFAPGGPVEQIIANLTG
                     PGGGANDRLGGGGGGDAGGGGNFEVGGDTGSKYRGAQGLDPEFIAKLEKQFGFDKPPL
                     ERFGKMLWDYARFDFGDSYFRDISVLDLILEKMPVSISLGLWITLISYLISIPLGIRK
                     AVKDGSAFDVWTSGIVIVGYAIPGFLFAILLMVLFAGGSFYDWFPLRGLVSDNWQQLS
                     WPARILDYFWHLVLPLTAMSLSAFATTTLLTKNSFLDEIRKQYVVTARAKGLSEAKVL
                     YGHVFRNAMLIVIAGFPGAFISAFFTGSLLIENIFSLDGLGLLGFRSIVERDYPVVFA
                     NLYIFSLIGLAVSLISDLTYTWIDPRIDFERRDV"
     gene            complement(2498..4327)
                     /locus_tag="VGM46_06925"
     CDS             complement(2498..4327)
                     /locus_tag="VGM46_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013527940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY4192363.1"
                     /translation="MAAGAAALLPGKAFADTAIGTKLHGLSAFGDLNYPADYPHFAYV
                     NPDAPKGGAMNFSPPNWTFNQSPLTFNTLNSFALKGDAPPRMEMCFDSLMARALDEPD
                     AVYGLLAESVTLSADRNSFDFTLRTEARWHDGTKLTAHDVAFSLMLYKDKGHPDLLLP
                     LVELKEAVALDDRTARLVFSGRQSAQTILSVVTMPVVSRAYYSANDFEAAQMKPPLGS
                     GPYKVGRVSAGQFIEYERAADYWANDLAINRGQYNFDRIRIDFYRDRQAAFEAFKKGD
                     TTFREEFTARVWTTGYDFPAFKQGKVVKREFPGEKWPAMQAIAINQRRERFRDVRVRR
                     ALDLCFDFEWTKRTLFSGSYDRSQSNFEHSDFRADGLPSPQELALLEPFRKELPPEAF
                     GEAVMQPVSDGTGRDRRLLGQASKLLSEAGWKRAGRFVVNGKGERLQVEMLAQDDSLV
                     RIASPWIDNMKAIGIDASVRLVDSSQYEERQSNFDFDLNMLAVSIGATPTADSLESLY
                     SSHAADRPGTRNYPGTKSPAIDALVAAAGRAQSRTELVTALKALDRVLRARLDWIPTY
                     YLANHRAAYWDMFGFVEQKPDFGFPVEALWWFDKDKAAKIGKG"
     gene            complement(4366..>5016)
                     /locus_tag="VGM46_06930"
     CDS             complement(4366..>5016)
                     /locus_tag="VGM46_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY4192364.1"
                     /translation="PVYDTPQAERRNLGHAVKLFEQAGWVIKSGKMVNAQSGEPFKLE
                     ILGRDPTDEVIANPFIDNLKKIGVSASLRIVDPSQYVNRVRGFDFDVVTGLFGQSDSP
                     GNEQRDFWSSKAADTPGSRNLMGIKSPVVDALIDRVIYATDRDDLVTATHALDRVLLW
                     NYYVVPQWYRSVVWLAYWNKFGIPDKQPAYIGADIDSWWIDPAREKALAAKYKSAN"
BASE COUNT         1045 a         1486 c         1606 g          879 t
ORIGIN      
        1 ctcatttgaa tgtcttgcgc ggatcgaagg cgtcgcgggt ggcttcgccg acgaagatca
       61 gcagcgacag catgatcgac agggtgacga agccggtgat gccgagccat ggcgcgttga
      121 gattgcgctg gccctgcttc aagagttcgc cgagcgaggc cgaacccggc ggcaggccga
      181 agccgaggaa atcgagcgag gtgagcgtcg agatcgagcc gttgaggatg aagggcagga
      241 aggtcagcgt cgccaccatg gcgttgggca acaggtggcg gaacatgatg gtgcggtttg
      301 gcacgccgag cgcgcgcgcc gcattgacat attcgaaatt gcgggcgcgc aggaattccg
      361 cccgcacgac gccgacgaag gccacccagg agaacaaaag catgatgccg agcaggatga
      421 agaagccggg cggtaggatg gcggcgacga tcagcagcag gtagagcacc gggattgccg
      481 accagatctc gatgaagcgc tggaacagaa gatcgatcca gccgccgaaa tagccctgca
      541 ccgcgccggc ggagacgccg atgacggccg agccgatggt gaggatgagg ccgaacagca
      601 ccgagacacg gaagccgtag atggcgcgcg ccagcacgtc gcgcgcctgg tcgtcggtgc
      661 cgagccagtt ccagttgccg atattgcagt cgggatcgtc gacgcccttg ggatattggg
      721 cgcagcgcgc cttggcgtca tattcccatg atggctttgc cggggccgcc tcgggaatgg
      781 cgttgttgac ggtggtgtag gaatagcgga tgggcggcca gaccatccag ccattggcct
      841 cgatctcgtc atggatgacc ggatcgcggt agtccgcgac cgcataaaag ccgccgaact
      901 tctcctccgg ataatcgacc agaacgggga aaagtatctc gcccttgtag gaagcgatga
      961 tcggcttgtc gttggcgatg aactcggaaa acagcgacag gacgaacagc agcaggaaaa
     1021 tccacagcga ccagtagccc cggcggttgg ccttgaaact ttgccagcgg cgctggttga
     1081 gcggtgacag ccaaggccgc gcgacgggtt cgcgcgcctg ttcgaccgcc gatttcaact
     1141 ggatatccga catcacacgt ctctccgctc gaagtcgatg cgcggatcga tccaggtata
     1201 ggtgagatcg gagatgaggc tgacggcaag gccgatgagc gagaagatat agaggttggc
     1261 gaacaccacc ggatagtcgc gctcgacgat ggagcggaaa ccgagcaggc caaggccgtc
     1321 cagcgagaag atgttttcga tcaacagcga gccggtgaag aaggctgaga tgaaggcgcc
     1381 ggggaaaccg gcgatgacga tcagcatcgc attgcggaag acgtggccat acagcacctt
     1441 tgcctcggac aagcccttgg cgcgcgccgt caccacatat tgcttgcgga tttcatcgag
     1501 gaaggaattc ttggtcaaaa gcgtcgtggt ggcgaaggcc gacaatgaca tcgctgtcag
     1561 cggcagcacc agatgccaga aataatcgag gatgcgcgcc ggccaggaaa gctgttgcca
     1621 attgtcggaa accagcccgc gcaatgggaa ccagtcgtag aacgagccgc cggcaaacag
     1681 caccatcagc aggatggcga acaggaagcc cggaatggcg tagccgacga tgacgatgcc
     1741 gctggtccag acgtcgaagg ccgagccgtc cttgaccgcc ttgcggatgc cgagcgggat
     1801 ggaaatcagg taggagatca gcgtgatcca cagaccgagc gaaatcgaca ccggcatctt
     1861 ttcgaggatc aggtcgagca cggaaatgtc gcggaaatag ctgtcgccga agtcgaaacg
     1921 ggcataatcc catagcatct tgccaaagcg ctcaagcggc ggcttgtcga agccgaactg
     1981 cttttccagc ttggcgatga attccgggtc cagcccctgc gcgccacggt atttggagcc
     2041 ggtgtcgccg ccgacctcaa aattaccgcc gccgccggca tcgccgccgc ccccgccgcc
     2101 aaggcggtca ttggcgccgc cgcccggtcc ggtcaaattg gcgatgatct gctcgaccgg
     2161 gccgccgggc gcgaactgga tgacggcgaa ggatatggcc atgatgccga acagggtcgg
     2221 gatcatcaac aggatgcggc gaaggatata ggcgcccatc agctcttacc ctccccgttg
     2281 tgaggaggga cgataagcga agcagggtgc gagcgcagac gacgccaagg cccctccccg
     2341 gcttacggac ttcgctgcgc taaccctgcc agctgaccca aaggaaaagg ctaaagcaca
     2401 cggcatggaa tttcccccgg ccttatgctt tcgctgcggg cgttcgtcgt tcgaaaggcc
     2461 aaatcgttgg cttttcgtcc gcattgcagg ccactcctca ccctttgcca atcttcgcag
     2521 ccttgtcctt gtcgaaccac cacagcgctt ccaccggaaa gccgaaatcg ggcttttgct
     2581 cgacgaaacc gaacatatcc caataagcgg cgcggtgatt cgccagatag tatgttggaa
     2641 tccagtcgag ccgcgcacgc aagacccggt cgagcgcctt cagcgccgta accagctcgg
     2701 tgcgactttg cgcacggccg gcggcggcga ccagcgcatc gatggccggg cttttcgtgc
     2761 cgggatagtt gcgcgtgccc ggcctgtcgg cggcatgcga ggaataaagc gattccaggc
     2821 tgtcggcggt cggcgtcgcg ccgatggaaa ccgccagcat gttcaaatcg aaatcgaaat
     2881 tgctctgccg ctcctcatat tggctggaat cgaccagccg gaccgaggcg tcgatgccga
     2941 tggccttcat gttgtcgatc cacggcgagg cgatgcggac cagcgaatcg tcctgcgcca
     3001 gcatttcgac ctgcaagcgc tcgcccttgc cgttgacgac gaaacggccg gcgcgtttcc
     3061 agcctgcctc ggaaagcagc ttcgaggcct gcccgagcag cctgcgatcg cggcccgtgc
     3121 catcggaaac gggctgcatg accgcttcgc caaacgcttc gggcggcaat tcttttcgga
     3181 acggttccag cagcgccagt tcctgtggcg aaggaagccc gtcggcgcga aagtccgaat
     3241 gctcgaagtt cgactgcgaa cggtcgtagg agccggaaaa cagcgtacgc ttcgtccatt
     3301 cgaagtcgaa gcaaagatca agcgcccggc gcacgcgcac gtcgcgaaaa cgctcgcggc
     3361 gctggttgat ggcgatggcc tgcatggcgg gccatttctc gccgggaaat tcgcgcttga
     3421 ccaccttgcc ttgcttgaag gctggaaaat catagccggt ggtccagaca cgggcggtga
     3481 attcctcgcg gaaggtggtg tcgcctttct tgaaggcttc gaatgcggcc tgccggtcgc
     3541 ggtagaaatc gatgcggatg cggtcgaaat tatattggcc gcgattgatg gcgagatcgt
     3601 tcgcccaata atcggccgcg cgctcatatt cgatgaactg gccagccgaa acgcggccga
     3661 ccttataagg cccggagccg agcggcggtt tcatctgcgc ggcttcgaaa tcgttggccg
     3721 agtagtaggc ccttgaaacg acaggcatgg tgacgacgct caggatggtt tgcgccgatt
     3781 gcctgcctga aaagacaagc cgcgcggtgc ggtcatccag cgctacggcc tccttcagtt
     3841 ccaccagcgg cagcaacaaa tcggggtggc ccttgtcctt gtagagcatc agcgagaagg
     3901 ccacgtcatg cgccgtcagc ttcgtaccgt catgccagcg ggcctcggtt cgcagcgtga
     3961 aatcgaaact gttgcggtct gccgacagcg tgacgctctc ggccagcagc ccatagacgg
     4021 catccggctc gtccagcgcg cgcgccatca ggctgtcgaa gcacatttcc atgcgcggcg
     4081 gcgcatcgcc cttcagcgca aaggaattca gcgtgttgaa ggtcagcggg ctttggttga
     4141 acgtccagtt gggcggcgag aaattcatcg ccccgccctt cggcgcatcc gggttcacat
     4201 aggcaaaatg tgggtagtct gccggatagt tgaggtcgcc gaaggccgac aggccgtgca
     4261 acttcgtgcc gatcgcggta tcggcgaatg ccttgccggg caacagcgcc gccgcacccg
     4321 ccgccatgcc cagcgccaga aagtcgcggc gcgcaagaag cccgctcaat tagcgctctt
     4381 gtacttggca gccagcgcct tttccctggc cggatcgatc caccaggaat cgatgtcggc
     4441 gccgatatag gcaggctgct tgtcgggaat gccgaacttg ttccaatacg ccagccacac
     4501 caccgagcga taccattgcg gcaccacgta ataattccac agcagcaccc ggtcgagcgc
     4561 atgggtggcg gtgacgaggt cgtcgcggtc ggttgcatag atgacgcggt cgatcagcgc
     4621 gtcgacaacg ggggacttga tgcccatcag gttgcgcgaa ccgggtgtgt cagcggcctt
     4681 cgaactccag aagtcgcgct gctcgttgcc gggcgagtcg gactggccga acaggccggt
     4741 caccacatcg aaatcgaagc cgcgcacccg gttcacatac tggctcggat cgacgatgcg
     4801 cagcgaggcg ctgacgccga tcttcttcag attgtcgatg aacggattgg cgataacctc
     4861 gtcggtcggg tcgcggccga ggatttccag cttgaacggc tcgccgctct gcgcattgac
     4921 cattttgccg ctttttatga cccagccggc ctgctcgaac agttttacgg cgtggccgag
     4981 gtttctacgc tccgcctgcg gcgtgtcgta gacggg
//
LOCUS       DASYCS010000216         1899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_195716,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000216 DASYCS010000000
VERSION     DASYCS010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195716"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            271..1005
                     /locus_tag="VGM46_06935"
     CDS             271..1005
                     /locus_tag="VGM46_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009364769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY4192365.1"
                     /translation="MADQLTGKTVVITAAAQGIGRASALAFAGAGATVYATDINEALL
                     AELGKVAGITARKLDVLDDNAVKAAFADIGRVDVLFNCAGFVHSGSILEMKDTELEFA
                     FDLNVHAMVRTIRAALPGMLERGDGAIINMSSVASSQKGVPNRFAYGLTKAAVIGLTK
                     AVAADYIGKGIRCNAICPGTVESPSLEGRMRAAGDYEKSRAAFIARQPIGRLGTAEEI
                     ADLAVYLAGATYTTGQAYNIDGGWSL"
     gene            1180..>1899
                     /locus_tag="VGM46_06940"
     CDS             1180..>1899
                     /locus_tag="VGM46_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018244172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY4192366.1"
                     /translation="MKLLRYGEVGAEKPGLLDADGTIRDLSKHVADIGGKALAPASLA
                     SLAGLDPKSLPAVSGKPRIGACVSGVGKFICIGLNYSDHAAETGAAVPSEPVVFMKAT
                     SAIVGPDDDVLIPRGSEKTDWEVELGVVIGKTAKYVSEAEALDYVAGYCVSHDVSERA
                     FQAERQGQWTKGKSCDTFGPIGPWLVTKDEVADPQNLKMWLTVNGESRQNGSSRTMVY
                     GVAFLVSYLSQFMSLHPGDVIS"
BASE COUNT          368 a          589 c          591 g          351 t
ORIGIN      
        1 tttcgcacct tccaggtgga ccagcttgcg tcggtccggg aaactttccg taaattcata
       61 ggccgccatc cgggctacaa ccggcgtgat cgcctgttct cgcgcgtgct gtgccaaata
      121 tgagcgcgct cgcggttgac gtcatgagat atgttcttta accagaaaaa acaggcttga
      181 aagccgcatc caggccaacg atcatgccgt cctggcgcat gacaacgtcc gcgcaggctg
      241 cgaaaaaggc cgatctgaga ggaagcacga atggcggatc aactgaccgg aaagacggtc
      301 gtcataacgg cggcggcgca gggtataggc agggcgagcg cgctggcatt cgccggggcc
      361 ggggccactg tctacgcaac ggacatcaac gaggcgctgc tggccgaact gggcaaggtc
      421 gcgggcatca ccgcgcgcaa actcgacgtg ctcgacgata acgccgtcaa ggcggctttc
      481 gccgatatcg gccgggtcga tgttctgttc aactgcgccg gctttgtcca ttcaggctcg
      541 atcctcgaaa tgaaggatac cgaactggag ttcgccttcg acctcaacgt tcacgccatg
      601 gtgcgcacca tccgcgccgc gctgccgggc atgctggagc gcggcgacgg cgccatcatc
      661 aacatgtcgt cggtggcgag ttcgcaaaaa ggggtaccaa accggttcgc ctacggcctt
      721 accaaggcgg cggtgatcgg cctcaccaag gcggtggccg ccgattacat cggcaagggc
      781 attcgctgta acgccatttg tccaggcacc gtggaaagcc cctcgctcga agggcgcatg
      841 cgggcggcgg gcgactacga gaaatcccgc gccgccttca tcgcgcgcca acccattggc
      901 cggctcggca cggcggaaga aatcgccgac ctcgccgtct accttgccgg tgcgacctat
      961 acgaccgggc aggcctataa tatcgacggc ggctggtcgc tctgagaggc cattgaaaac
     1021 tctgttccgc caggggcctg acgcgacttt ctgcgcttcc ggtgctcaca tatttgaatg
     1081 taagctccgc tcgtcgccta tggcgacccc aaaccacgtc attcgcctca tcggagcgag
     1141 tttgcaaaag gcctctggcc gcaaccggga gaaaaatcca tgaaactgtt gcgctatggc
     1201 gaagttggcg ccgaaaaacc cggcctgctc gacgcggatg gaacgatccg cgatttgtcg
     1261 aagcatgtgg ctgacatcgg cggcaaggcg ctggcccccg cttcgctggc gtcgcttgcc
     1321 gggcttgatc cgaaatcgct gccggcggtg tcgggcaagc cgcgcatcgg cgcctgtgtt
     1381 tccggcgttg gcaaattcat ctgcatcggg ctgaattatt ccgaccatgc cgccgaaacc
     1441 ggcgctgccg tgccttccga accggtggtg ttcatgaagg cgacctcggc catcgtcggc
     1501 ccggacgatg acgtgttgat cccgcgcggt tcggaaaaga ccgattggga ggtcgagctt
     1561 ggcgtcgtca tcggcaagac cgccaaatat gtttccgagg ccgaggcgct ggattacgtg
     1621 gccggctact gtgtctcgca cgatgtgtcc gagcgcgcct tccaggctga gcggcaaggc
     1681 cagtggacca agggcaagtc ctgcgacacc ttcgggccca tcggcccgtg gctggtgacg
     1741 aaggacgagg tggccgaccc gcagaacctg aagatgtggc tgaccgtcaa tggcgaaagc
     1801 cgccagaacg gctcgtccag aacgatggtc tacggcgtcg ccttcctggt ctcttatctc
     1861 agccagttca tgagcctgca tcccggcgac gtcatctcc
//
LOCUS       DASYCS010000217         4477 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1209048,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000217 DASYCS010000000
VERSION     DASYCS010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4477
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1209048"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..738)
                     /locus_tag="VGM46_06945"
     CDS             complement(151..738)
                     /locus_tag="VGM46_06945"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006206384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEY4192367.1"
                     /translation="MPHVEKGMQVGLFGGSFNPPHAGHALVADIALRRLALDQLWWMV
                     TPGNPLKSARELAPLAERLRLSEEIARDPRIKVTAFEAAHHVRYSADTLALVRRKNPG
                     VDFVWIMGADSLRDFHRWQRWRQIMLTFPIAVIDRPGSTLSFLSSVVAKTFDYARVDE
                     GDAPRLAHMRAPAWTFIHGPRSLLSSSAIRQAAGE"
     gene            complement(905..2191)
                     /locus_tag="VGM46_06950"
     CDS             complement(905..2191)
                     /locus_tag="VGM46_06950"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEY4192368.1"
                     /translation="MLNQHDRSQTDIATLMADIGARARAAARPLAIASAERKYAALVG
                     MAQAIARREQDILDANAIDMANGEDAGLSPALLDRLKLTPARIRAIADGIRAIAELRD
                     PIGEVIAQWDRPNGLSIERVRTPLGVIGVIYESRPNVTADAGALCLKAGNPVILRGGT
                     DSANSSAAIHACLVEGLKNASLPQDAIQLVPTTDRAAVGEMLKGLSGNLDVIIPRGGK
                     SLVSRVQNEARVPVFAHLEGICHLYIDKSADLDMAVKIAVNAKMRRTGICGAAETLLV
                     DRAVAATHLSPILSALQNAGCEIRADAEVLEAFPAASPATDADWTTEYLDAIISVKLV
                     DGVVAAIDHIENFSSHHTEAIVAEDAQAVEKFFNEIDSAILLHNASTQFADGGEFGMG
                     AEIGIATGKMHARGPVGVEQLTSFKYRVRGTGQVRP"
     gene            complement(2175..3332)
                     /gene="proB"
                     /locus_tag="VGM46_06955"
     CDS             complement(2175..3332)
                     /gene="proB"
                     /locus_tag="VGM46_06955"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006206380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEY4192369.1"
                     /translation="MTALSVKKYRRIAIKIGSALLVDRTSGLKRDWLASLARDIGELA
                     EGGAEILVISSGAIALGRTILGLGKRALKLEESQAAAAVGQIALAGAWSDALGGHGLK
                     SGQILLTLGDTEERRRYLNARATISTLLKMKAVPVINENDTVATSEIRYGDNDRLAAR
                     VATMMGADLLVLLSDIDGLYTAPPAHDPNARFIPLVEHITPDIEAMAGAAASELSRGG
                     MRTKLDAGKIATAAGTAMIITAGTRLSPLMAIERGERATFFRPSAHPVKGYKTWIAGQ
                     LEPVGRLSVDAGAVSALLSGKSLLPAGVKLVSGNFARGDTVAILSPQGREIARGLVAY
                     DAADAVRIAGLKTTEIETVLGYEARSAMIHRDDLVVSGARDMTENDHAEPA"
     gene            complement(3340..4368)
                     /gene="obgE"
                     /locus_tag="VGM46_06960"
     CDS             complement(3340..4368)
                     /gene="obgE"
                     /locus_tag="VGM46_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006467868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEY4192370.1"
                     /translation="MKFLDQAKVYIRSGDGGAGSVSFRREKFIEFGGPDGGDGGRGGD
                     VWVETVNGLNTLIDYRYQQHFKAKTGMHGMGRNMTGGKGADVTLKVPVGTQILEEDNE
                     TLICDMVRLGQRYLLAKGGNGGFGNQHFKTSTNQAPRRANPGLEGTEMNIWLRLKLIA
                     DAGLVGLPNAGKSTFLAAVTAAKPKIADYPFTTLHPGLGVARIDAREFVIADIPGLIE
                     GAHEGVGIGDRFLGHVERTRVLLHLISAQEENPGKAYKTVRAELDAYGNGLTEKVEIV
                     ALSQVDILDADERKKKLASLKRACGRAPMLLSAVTGEGVEAVLRALMKIVEEAREPTA
                     PADTRWQD"
BASE COUNT          741 a         1395 c         1540 g          801 t
ORIGIN      
        1 agaacaccgg cgggacgcga ggttcgcgcc acgtaactta tcactctgga aggctcggcc
       61 tcgcgccccg cttcccacct gcccctgcat aagggcagcg ttccttgaca aaggccagac
      121 ggcgaatgcc gggcgtggca atgaagacgg ctattcgccg gccgcctgcc ggatcgcgct
      181 ggacgacagc agcgagcgcg ggccgtggat gaaggtccag gccggcgcgc gcatatgggc
      241 aaggcgtggc gcatcgcctt catcgacgcg ggcataatcg aaggttttgg ccaccaccga
      301 cgacaggaac gacagcgtcg agccgggccg gtcgatcacg gcgatcggaa aggtcagcat
      361 gatctggcgc cagcgctgcc agcggtggaa atcgcgcagc gaatccgcgc ccatgatcca
      421 gacaaaatcg acgcccggat ttttcctgcg caccagcgcc agcgtgtcgg cgctatagcg
      481 gacgtggtgg gcggcctcga aggcggtgac cttgatgcgc gggtccctgg cgatttcttc
      541 cgaaaggcgt agccgctcag ccagcggggc aagttcgcgc gcgcttttca gcggattgcc
      601 gggcgtcacc atccaccaca gctgatcgag cgccagcctt cgcagcgcaa tgtcggcaac
      661 cagcgcgtgg ccggcgtggg gcggattgaa cgagccgccg aacaggccga cctgcatgcc
      721 tttttcgaca tgcggcatat gcaggtaatg ggaaggaatg gccgctccat cccgcccctt
      781 gccgggaagg tcgatccgcg aagcgggacg ggaaggaggc gtttgggaat tcagcgtaat
      841 gcccccaccc ggttcgtaat gcttgccacc ctcacgatgc gggggaaggc aaaacgggcg
      901 gaagtcacgg ccgcacctgg cccgttccgc gaacgcggta tttgaacgag gtgagctgtt
      961 cgacgccgac agggccgcgc gcgtgcatct tgccggtggc gatgccgatt tcggcgccca
     1021 tgccgaattc gccgccatcg gcgaactggg tcgaggcatt gtgcagcagg atcgcggaat
     1081 cgatctcgtt gaagaatttt tccaccgcct gtgcgtcttc ggcaacgatg gcctcggtgt
     1141 ggtgcgagga aaaattctcg atgtggtcga tggcggcgac gacgccgtcg acgagcttca
     1201 ccgagatgat ggcatcgaga tattcggtgg tccagtcggc gtcggttgcc gggctggcgg
     1261 cggggaaagc ctccagcacc tcggcatcgg cgcggatttc gcagccggca ttctgcaagg
     1321 cggaaaggat cggcgacagg tgggtggcgg cgacggcgcg gtctaccagc agcgtctcgg
     1381 cagcgccgca aatgccggtg cgccgcatct tggcgttgac ggcgattttc accgccatgt
     1441 cgaggtccgc cgatttgtcg atgtaaagat ggcagatgcc ctccagatgc gcgaaaaccg
     1501 gcacccgcgc ctcattttgc acgcgcgaga cgaggctttt gccgccgcgc ggaatgatga
     1561 cgtcgagatt gccggacagg cccttcagca tctcgccgac ggcggcgcgg tcggtggtcg
     1621 gcaccagctg gatggcgtct tgcggaaggc tggcgttttt caggccttcg acgaggcagg
     1681 cgtggatggc ggcggaggaa ttggccgaat ccgtgccgcc gcgcaggatc accgggttgc
     1741 cggccttcaa gcacagcgca ccggcatccg cggtgacgtt cggacggctc tcatagatga
     1801 cgccgatgac gccgagcggc gtgcgcacgc gttcgatgga aaggccgtta ggacggtccc
     1861 attgtgcgat gacctcaccg atcgggtcgc gcagttcggc gatggcgcgg atgccgtcgg
     1921 cgattgcgcg aatgcgggcg ggcgtcagct tgaggcggtc caagagggca ggggaaaggc
     1981 cggcgtcctc gccattggcc atgtcgatgg cgttggcgtc gagaatatcc tgctcgcgcc
     2041 gcgcaatcgc ctgcgccatg ccgacaagcg ctgcatattt acgctccgcg ctggcgatgg
     2101 ccagcggtcg ggcagcagcg cgggcgcggg cgccgatgtc ggccatcagc gttgcgatat
     2161 cggtttgcga ccggtcatgc tggttcagca tggtcattct ccgtcatgtc acgcgcgccg
     2221 cttaccacga ggtcgtcgcg gtgaatcatt gccgaacggg cttcgtagcc gagcaccgtt
     2281 tcgatctcgg ttgttttcag ccccgcgatt cttacggcat cggcggcatc ataggcaacc
     2341 agcccgcggg cgatctcgcg gccctgcggc gacaggatgg caaccgtgtc gccgcgcgcg
     2401 aaattgccgc tgaccagctt gacgccggcg ggcagcagcg atttgccgga aagcagcgcg
     2461 ctgaccgcgc cggcgtcgac ggagagccgc ccgaccggtt cgagctgacc ggcgatccag
     2521 gtcttgtagc ctttgacggg atgggcgctc ggcctgaaga aagtggcgcg ctcgccgcgc
     2581 tcgatcgcca tcagcggcga aagccgcgtg ccggcggtga tgatcatggc ggtgccggcg
     2641 gcggtggcga tcttgccggc gtcgagcttg gtgcgcatgc cgccgcgcga cagttcggag
     2701 gcggcagcgc cggccatcgc ctcgatatcc ggcgtaatgt gctccaccag cggaatgaag
     2761 cgcgcattgg ggtcgtgcgc cggcggggcg gtataaaggc cgtcaatgtc ggaaagcagc
     2821 accagcaggt ccgcgcccat catggtggcg acgcgcgccg ccagccggtc gttgtcgccg
     2881 tagcggattt ccgaggtggc gacggtgtcg ttctcgttga tgaccggcac cgccttcatc
     2941 ttgagcagcg tggagatggt ggcgcgggca tttagatagc ggcggcgctc ctcggtgtcg
     3001 cccagcgtca gcaggatctg gccggatttc aggccatggc cgccaagcgc atccgaccat
     3061 gcgccggcca gcgcgatctg gccgaccgca gcggccgcct ggctttcctc aagcttcaag
     3121 gcgcgcttgc ccaggcccag aatggtgcgg ccaagcgcaa tggcgccgga ggagataacg
     3181 aggatttcag cgccgccttc ggccagttcg ccaatatcgc gcgccagcga agcgagccag
     3241 tcgcgcttga ggccggacgt gcggtcgacc agcagggccg agccgatctt gatggcgatg
     3301 cgccggtatt ttttgaccga tagagccgtc atggcgcggc tagtcctgcc accgcgtgtc
     3361 ggccggcgcg gtcggttcgc gggcttcttc aacgatcttc atcagggcgc gcagcaccgc
     3421 ctctacgccc tcgccggtga cggccgacaa aagcatcggc gcccgcccgc aggcgcgctt
     3481 cagggacgcg agcttcttct tgcgctcgtc cgcatcgaga atatcgacct ggctgagcgc
     3541 gacgatctca accttttcgg tcagcccgtt gccgtaggca tcgagttcgg cgcgcacggt
     3601 cttgtaggcc ttgccggggt tttcttcctg cgccgaaatc agatgcagaa ggacgcgcgt
     3661 gcgctcgaca tggccgagga agcggtcgcc gatgccgacg ccttcatgcg cgccctcgat
     3721 caggccggga atatcggcga tgacgaattc gcgggcgtcg atgcgcgcaa cgccaaggcc
     3781 gggatgcagc gtggtgaagg gatagtcggc gatcttcggc ttggccgccg tcaccgccgc
     3841 gaggaaggtg gacttgccgg cattgggcag gccgaccagc ccggcgtcgg caatcagctt
     3901 cagccgcagc cagatgttca tctcggtgcc ttcgaggccg ggattggcgc ggcgcggcgc
     3961 ctggttggtc gaggtcttga aatgctggtt gccgaagccg ccattgccgc ccttggccag
     4021 cagatagcgc tggccaagcc gcaccatgtc gcagatcagc gtctcgttgt cttcttccag
     4081 aatctgcgtg ccgaccggca ccttcagcgt cacgtcggca cccttgccgc cggtcatgtt
     4141 gcggcccatg ccgtgcatgc cggtcttggc cttgaaatgc tgctggtagc ggtagtcgat
     4201 gagcgtattc agcccattga ccgtctcgac ccagacatcg ccgccgcggc cgccgtcgcc
     4261 gccatccggc ccgccgaatt cgatgaattt ttcgcgccgg aacgacaccg aaccggcgcc
     4321 gccgtcgccg gagcgtatgt agactttggc ttgatcgaga aatttcatcg caaagggctg
     4381 tttctgctgg ggttggtttg gtcgagcgtc ctcatctatc ttatagcgcg gccaagggcg
     4441 agagtggaca cgccgtgttt tggcgtgtcc ctgtggc
//
LOCUS       DASYCS010000218         7198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_486882,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000218 DASYCS010000000
VERSION     DASYCS010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 7198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7198
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486882"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="VGM46_06965"
     CDS             complement(<1..483)
                     /locus_tag="VGM46_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006204987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY4192371.1"
                     /translation="MLKRLDIAIAGAGPAGLAVALYLKRAGHRVTVFERFKQTAPVGS
                     GLILQPTGLTVLADLGLLDQILVLGSRIDRLHGADAGSGRTVLDVRYDARSGGRFGLA
                     VHRAALFGVLFRAAQRENIAIETATELETLDAGERATLVCQDGKRIGPFDLVVDASGS
                     N"
     gene            complement(565..2478)
                     /locus_tag="VGM46_06970"
     CDS             complement(565..2478)
                     /locus_tag="VGM46_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HEY4192372.1"
                     /translation="MALANNAGEAETIDPPSQPEVEQHGTKVLMLGALGVVYGDIGTS
                     PIYAFREALHSSSGPFLPENVLGVLSLIVWALTIIVTVKYVAFVLRADNKGEGGTLSL
                     MALARSAYPNGAKFILGIGLCGAALFFGDAVITPAISVLSAVEGLQVIAPTLNTYIVP
                     ITLVILAMLFSVQRFGTGRVAVVFGPIMALWFLVLGIAGLVHIWDDPSVLWAINPYHA
                     LAYLFDEPDVAFITVGAVFLAVTGAEALYVDLGHFGRRPIVLTWFAIVFPCLLLNYFG
                     QGAFVLSHGMVPENPFFQMLPPWALAPMVGLATAATVIASQAVISGAFSLTRQAVQLN
                     LLPRIEVQHTSEMQLGQIYMPRVNFIIAVVVMLLVVGFGDSHSLASAYGISVTGEMLM
                     TTILLFVVMRSLWKWHVGIALGLALVFGIIDTGFFLANVAKVMDGGWVSISVACVMGV
                     IMVTWIKGSRYLFDKTRKNEVPLDFLAGNLLKKQPHLVPGTAIFLTSDPLSAPTALMH
                     SLKHYKVLHEQNVILSVVTAQQPMVPDSERIKMEALNDLFMRVTLTFGYMEHPNIPRA
                     LAACRKKGWKFDIMTTSFFLSRRSLKASPNSGMPMWQDRLFIGLARTAADATEYFQIP
                     TGRVVEIGTQVAI"
     gene            complement(2580..3320)
                     /locus_tag="VGM46_06975"
     CDS             complement(2580..3320)
                     /locus_tag="VGM46_06975"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEY4192373.1"
                     /translation="MPAKLSVNLNAVAMLRNRRALPWPSVAGLGRIALAAGAHGLTVH
                     PRPDERHTRHSDLPEIRALIDDEFPKAEFNIEGYPSEDFLALVEKNQPEQVTLVPDDP
                     SQATSDHGWNFAEKADFLAPVVKRLKGSGFRVSLFCDPDPAIASVAKDLGADRIELYT
                     GPYGSFHLDAGKTANELEKLGKTADAALAAGLQVNAGHDLVVANLPALVKRIPNLAEV
                     SIGHGLTADALEYGMAATVRRFLNACGW"
     gene            3319..3837
                     /locus_tag="VGM46_06980"
     CDS             3319..3837
                     /locus_tag="VGM46_06980"
                     /inference="COORDINATES: protein motif:HMM:NF023390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HEY4192374.1"
                     /translation="MEKASKSVQRATYRTFDGNAQGDSGVPEAEKTPQTGEKTMNFLE
                     PVPAIRRIETDRSDLFAIDVVGHASAADAENLFGLLEAAYMLHPKIDVLVRLVDHQGV
                     DWADLSRQTLERGKTDALEHVRRCATIGEPNWIGQVQGLFTSLPTELRHFDADHEPQA
                     WQWLGAKPVEAA"
     gene            complement(3838..4965)
                     /locus_tag="VGM46_06985"
     CDS             complement(3838..4965)
                     /locus_tag="VGM46_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002964497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEY4192375.1"
                     /translation="MEFSPQQDEALKAVARWLKAGKPQLFRLFGYAGTGKTTLARYFA
                     EHVDGQVQFAAFTGKAAQVLRSKGATNARTIHSLIYRPKGEESVEDEVTGKTSMSPTF
                     SLNRQSPISRAALVVIDECSMVDEQLGRDMMSFGTPILVLGDPGQLPPISGGGFFTEH
                     EPDFLLTEIHRQARDNPIIRLALDVREGREFPRGDYGAARVIGKDEVNQELVLKADQV
                     LVGTNRTRRRYNQRLRELNGFTADYPQAGDKLVCLRNDPAKGLLNGSLWKVMTASRET
                     VKPGINLLVSPEEDDPDRGVAKIKLMKAVFEDPDADISWQQKKRFDDFDYGYALTVHK
                     AQGSQWNEIVLFDESFAFKETRQRWLYTAITRAAERLTIVR"
     gene            complement(5053..5736)
                     /locus_tag="VGM46_06990"
     CDS             complement(5053..5736)
                     /locus_tag="VGM46_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019444570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4192376.1"
                     /translation="MMAEAIIELKAVERHYVQGSRKLTILDGVDFSLRRGEMVALVAP
                     SGTGKSTLLHTAGLLERPDAGDVVLAGRACGRLSDDQRTKIRRNDVGFVYQFHHLLPE
                     FSALENIMMPQLIKGLKPKEAAERAEQLLDYMQIGKRATHRPAELSGGEQQRVAIARA
                     VANAPLVLLADEPTGNLDPVTASYVFEALAALVKQSGLAALIATHNHALAERMDRVVT
                     LANGKIVPL"
     gene            complement(5733..7022)
                     /locus_tag="VGM46_06995"
     CDS             complement(5733..7022)
                     /locus_tag="VGM46_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEY4192377.1"
                     /translation="MSDVTATKNAAAAGPFSVFERMVAWRYLRSRRKETVISVIASIS
                     FLGIMLGVATLIVVMAVMNGFRAELLTRILGVNGHLIVTPIDLPLDDYAAVAGRINGV
                     PGVKYAIPLIDGQVLAQGSVGSGTGALVRGIRGEDIGKISIVANNIKQGSLVGFDKGE
                     GVAIGSRMAESLGLVLGDTITLISPDGDVTPLGTTPRLKGYRIAAIFEVGMSEYDSSI
                     IYMPFSEAQLYFNMEGKAQTIEIYVDNPDDVDALKPKVEEAAQRQIFLTDWRQRNQTF
                     FSALEVERNVMFMILTLIVLVAALNIISGLIMLVKDKGHDIAILRTMGASRSAILRIF
                     LMTGAAIGVTGTLAGVGLGIVICLNIERIREFFSWILGTVLFNPELYFLSQLPAKMDF
                     SETFSVVLMALGLSFVATLFPAWRAARLDPVEALRYE"
     gene            complement(7019..>7198)
                     /locus_tag="VGM46_07000"
     CDS             complement(7019..>7198)
                     /locus_tag="VGM46_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019858921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HEY4192378.1"
                     /translation="TDQRPGGKFATADLIGLPWQFIVGPRGVAAGEVEIKNRRTGERE
                     TLPIASALQRFGATA"
BASE COUNT         1487 a         2295 c         2120 g         1296 t
ORIGIN      
        1 attcgagcca cttgcgtcca ccaccagatc gaacgggccg atccgcttgc catcctggca
       61 caccagcgtt gcgcgctcgc cggcatccag cgtttcaagc tcggtggcgg tttcgatggc
      121 gatgttttca cgttgggccg cacgaaaaag cacgccaaac agggccgcgc gatgcaccgc
      181 cagcccaaag cgcccgccgc tgcgcgcatc gtagcgcaca tcgagaacgg tgcggccgct
      241 gcctgcatcc gcgccgtgaa gccgatcgat acggctgccc agcaccagga tttgatccag
      301 caggccaaga tcggcaagaa cggtcagccc ggtcggctga aggatcaggc cggagccgac
      361 cggagccgtt tgcttgaacc gctcaaacac ggtgacgcgg tgacctgccc gcttgaggta
      421 gagcgcaacg gccaatccgg ccggcccagc cccggcaatt gcgatatcca gccttttcag
      481 cagccgattt ctcccccgcc tgcctgcccg gattgaggcg ttgaagcgcc cggtggcggc
      541 gtcaattcgc gatcccgcgc gatgctagat tgccacctgc gtgccgatct caacaacgcg
      601 cccggttgga atctggaaat attccgtggc atcggcggcg gtacgcgcca ggccgatgaa
      661 cagccggtcc tgccacatcg gcatgcccga attcggcgac gccttcagcg aacgccgcga
      721 aaggaagaac gaggtcgtca tgatgtcgaa cttccagcct ttcttccggc aagcggcaag
      781 ggcgcgcgga atattcgggt gctccatata accgaaggtc agcgtcaccc gcatgaacag
      841 gtcgttgagc gcctccatct tgatccgctc gctgtccggc accatcggct gctgggccgt
      901 caccacggac aagataacgt tctgctcgtg cagcaccttg taatgcttga ggctgtgcat
      961 cagcgccgtt ggcgcgctca agggatcgct ggtcaggaaa atggccgtgc cgggcaccag
     1021 atgcggctgc ttcttgagca ggttgccggc caggaaatcg aggggcacct cgttcttgcg
     1081 ggtcttgtcg aacagatacc ggctgccctt gatccaggtg accatgatca cgcccatgac
     1141 gcatgcgacg ctgatcgaca cccagccgcc atccatgacc ttggcgacat tggcgaggaa
     1201 aaatccggtg tcgatgatgc cgaataccaa cgccaggccg agcgcaatgc cgacatgcca
     1261 tttccacagc gaacgcatga ccacgaacag caggatcgtc gtcatcagca tctcgccggt
     1321 aacggagatg ccataagccg atgccagcga atgggaatcg ccgaaaccga cgacaagcag
     1381 catcacgacc accgcgatga tgaagttgac gcgcggcatg taaatctggc cgagctgcat
     1441 ctccgaggtg tgttggacct cgatgcgcgg caacaggtta agttgcaccg cctgcctggt
     1501 gagtgagaat gcgcccgaaa tgaccgcctg gctggcaatg acggttgcag cggtcgcaag
     1561 cccgaccatc ggcgccagcg cccacggcgg cagcatctgg aagaaaggat tttccggcac
     1621 catgccgtgg gacaatacga acgcgccctg cccgaaataa ttgagcagga ggcacggaaa
     1681 gacgatggcg aaccaggtca gcacgatcgg acggcggccg aaatggccaa ggtcgacata
     1741 gagcgcttcc gcaccggtca cggcaaggaa gaccgcgcca accgtgatga aggccacgtc
     1801 cggctcatcg aacagatagg ccagggcatg atatggattg atggcccaca acaccgacgg
     1861 atcgtcccat atatggacaa gcccggcgat gccgagcacc aggaaccaca gcgccatgat
     1921 cgggccgaac accaccgcca cgcgcccggt gccgaaacgc tgcaccgaaa acagcatagc
     1981 caggatgacc agcgtgatcg gcacgatgta ggtattcagc gtcggcgcga tgacctgaag
     2041 gccttcgacg gccgaaagca ccgaaatcgc cggcgtgatg acggcatcgc cgaaaaacag
     2101 cgctgcgccg cacaggccga tgcccagaat gaactttgca ccgttcggat aggcgctgcg
     2161 cgccagcgcc atcagcgaaa gcgttccgcc ctcgccctta ttgtcggccc gaagcacgaa
     2221 agcgacatat ttgacggtga cgatgatggt cagcgcccag acgatcagcg acagcacgcc
     2281 caatacgttc tcgggcagaa acggtcctga cgaggaatgc agcgcttcac gaaacgcgta
     2341 gatcgggctg gtgccgatat cgccatagac cacgcccagc gcgccgagca tcaacacctt
     2401 ggtgccgtgt tgctctacct cgggctggct tggtggatcg atggtctctg cctcaccagc
     2461 attgttggca agggccatgg acgacactcc gttaagcgcg gggtgctcta gccttgctgc
     2521 atcgcaatat caaggaccaa tgcatcgccg cctcgcatag ccccgatatg gaggcacctt
     2581 caccagccgc aagcgttgag aaaacgccgg actgtcgcgg ccatgccata ctccagcgca
     2641 tcggctgtca agccatgacc gatcgacact tcggcgagat tggggatacg ctttacaagc
     2701 gccggtaaat tggcgacgac aagatcgtgc ccggcattca cctgcaaccc tgccgccagt
     2761 gccgcatcgg ccgttttacc cagcttttcc agctcattgg ccgttttgcc tgcatctaaa
     2821 tgaaagctgc cgtaaggccc ggtataaagt tcgatcctgt ctgcgccgag gtctttcgca
     2881 acgcttgcga tcgccggatc gggatcgcaa aacaacgata cccgaaagcc gcttcccttc
     2941 aatcgcttga cgacaggcgc gaggaaatca gccttttcgg cgaaattcca gccatgatcc
     3001 gacgtcgcct ggctcgggtc gtccggcacc agcgtcacct gctcgggctg gtttttttcc
     3061 acaagcgcca gaaagtcttc gcttggatat ccttcgatgt tgaattccgc tttcggaaac
     3121 tcgtcatcga tgagcgcgcg aatctccggc aggtcggaat ggcgcgtatg ccgttcatcc
     3181 gggcgcggat gcacggtcag cccatgcgcg ccggcggcaa gcgcaattcg cccaagcccg
     3241 gccacgctcg gccatggcag cgcgcgccgg ttgcgcagca tggcgacggc attgaggttc
     3301 acggatagtt ttgcaggcat ggaaaaggcc tcgaaaagcg ttcaacgcgc cacctataga
     3361 acctttgatg ggaacgcgca gggggattca ggtgttccag aggcagaaaa aacacctcag
     3421 acaggggaaa agactatgaa ttttctggag ccagtaccgg cgatccgccg tatcgagacc
     3481 gatcgtagcg acctttttgc aatcgacgtc gtcggccatg catcggccgc cgatgccgaa
     3541 aatttgttcg gattgctgga ggcagcctac atgcttcacc ccaagatcga cgttctggtg
     3601 cgccttgtcg accaccaggg cgtcgactgg gccgatctgt cgagacagac gctggagcgc
     3661 ggtaagacag atgcgttgga gcatgtaagg cgatgcgcga caatcggcga accgaactgg
     3721 atcggccagg tccaaggcct tttcacctca ttgccgaccg aacttcgaca cttcgacgca
     3781 gatcatgagc ctcaggcgtg gcaatggctt ggcgcaaagc cggtggaggc agcctgacta
     3841 tcgcacgatg gtcaggcgct ccgccgcgcg cgtgatggcc gtatacagcc agcgctggcg
     3901 ggtttccttg aacgcaaagc tctcatcgaa cagcacgatc tcgttccatt gcgagccttg
     3961 ggctttgtgc accgtcagcg catagccata atcgaaatcg tcgaaccgct tcttttgctg
     4021 ccatgaaatg tcggcatcgg gatcctcgaa caccgctttc atcagcttta tcttggcgac
     4081 gccgcggtcc ggatcgtctt cttccggtga aacgaggagg tttatgccgg gtttgaccgt
     4141 ctcgcgtgag gccgtcatga ccttccacag cgagccgttc aaaagcccct tggcaggatc
     4201 gttgcgcagg caaaccagct tgtcgccggc ctgcggatag tcggcggtaa acccgttcaa
     4261 ttcgcgcagg cgctggttat aacggcggcg cgtgcggttt gtgccgacca gaacctgatc
     4321 ggctttcagc accagttcct ggttgacctc gtccttgccg atcacccgcg cggcgccgta
     4381 gtcgccgcgc gggaattcgc ggccctcgcg cacatccagc gccagccgaa tgatgggatt
     4441 gtcgcgcgcc tgccggtgaa tttcggtcaa aaggaaatcc ggttcatgct cggtgaagaa
     4501 acccccaccc gaaatcggcg gcaactggcc cggatcgcca agcaccagaa tcggcgtgcc
     4561 gaagctcatc atgtcgcggc caagctgctc gtccaccatc gagcactcat cgatgacgac
     4621 gagcgcggcc cgcgagatcg ggctttgccg gttgagcgag aatgtcggcg acatcgaggt
     4681 cttgccggtc acttcgtctt cgaccgattc ttcgcccttc ggccgataga tcagcgaatg
     4741 gatggtgcgg gcattggtcg cccctttcga gcgcagcacc tgcgccgcct tgcccgtgaa
     4801 ggcggcgaac tgtacttgcc catcgacatg ctcggcaaaa taacgcgcaa gcgtcgtctt
     4861 gccggtgccc gcatagccga acagcctgaa cagttgcggc ttgccggcct tgagccagcg
     4921 cgcgacggcc ttgagcgcct catcttgctg cggagaaaat tccatcaaat ggacagacag
     4981 gattcgccgg cacagggcaa ggtttttgcg tgcccacgcg ccgcctatcg ccgccgattg
     5041 tcgagaccgg acctatagag gcacgatttt tccattggcc agcgtgacga cacggtccat
     5101 ccgctcggcc agcgcatgat tatgggtggc gatcagcgcg gcgagaccgg actgcttgac
     5161 cagcgccgcc agcgcctcga acacgtaaga ggccgtgacc gggtcgagat tgccggtcgg
     5221 ctcgtccgcc agcagcacca gcggcgcgtt ggccacggcg cgcgcaatgg cgacgcgctg
     5281 ctgctcgccg cccgaaagct cggcaggccg gtgcgtcgcc cgcttgccga tctgcatgta
     5341 atccagcaat tgttcggccc gttccgctgc ttccttgggc ttcagcccct tgatcagctg
     5401 cggcatcatg atgttttcga gcgccgaaaa ctccggcaaa agatgatgga actggtagac
     5461 aaagccgaca tcgttgcggc gtatcttcgt gcgttgatcg tccgaaagac ggccgcaggc
     5521 ccggccagcc agcaccacat caccggcgtc gggacgctcc agtaggcccg ccgtatgcag
     5581 cagcgtcgac ttgccggtgc ccgaaggcgc aacaagcgcc accatctcgc cgcgcctcag
     5641 cgagaaatcc acgccatcga gaatggtaag cttgcgcgac ccctgcacat agtgccgttc
     5701 gaccgccttc aactctataa tggcctcggc catcattcat acctcaatgc ttcgacggga
     5761 tcgagccgcg cggcgcgcca tgccgggaac agggtcgcga cgaaggaaag cccaagcgcc
     5821 atcagcacga ccgaaaaagt ctcggaaaaa tccatcttgg ctggaagctg gctcaggaaa
     5881 taaagttccg ggttaaacag caccgttccc aatatccagg aaaagaattc ccgtatccgc
     5941 tcgatattca ggcagatcac gatgcccaac cccacccccg ccagcgtgcc ggtgacgccg
     6001 atggccgccc ccgtcatcag gaagatgcgc aggatcgccg agcgcgaggc gcccatcgtg
     6061 cgcaggatgg caatgtcgtg gcctttgtcc ttcaccagca tgatgaggcc cgaaataatg
     6121 ttcagcgccg ccaccagcac gatcagcgtc aggatcatga acatcacatt gcgctcgacc
     6181 tcgagcgccg aaaagaaggt ctggttgcgc tggcgccagt cggtcaggaa tatctggcgt
     6241 tgcgccgcct cttccacctt cggtttcagc gcgtcgacat cgtcggggtt gtcgacatag
     6301 atttcgatcg tctgcgcttt gccttccatg ttgaaataga gctgcgcttc ggaaaacggc
     6361 atgtagatga tcgaactgtc atattccgac atgcccactt cgaagatcgc cgcgatccga
     6421 tagcctttca ggcgcggcgt cgtgccaagc ggcgtcacat ccccgtccgg cgaaatcagc
     6481 gttatggtgt cgcccagcac aagccccagg ctctccgcca tccggctgcc gatggcgacg
     6541 ccctcgccct tgtcgaagcc aacgagggag ccttgcttga tattattggc gacgatggaa
     6601 atcttgccga tatcctcgcc gcgaatgccg cgcaccagcg cgccggtgcc gctgccgacg
     6661 ctgccctgcg ccaacacctg cccgtcgatc agcggaatgg cgtatttgac gccgggcacg
     6721 ccattgatgc ggccggccac ggccgcgtaa tcatccagcg gcaggtcgat aggcgtcacg
     6781 atcaggtggc cgttgacgcc gagaatgcgc gtcagaagct ccgcgcgaaa accgttcatc
     6841 accgccatca ccactatcag cgtggcaacg cccagcatga tgcccagaaa cgagatggag
     6901 gcgatgaccg agatcaccgt ttccttgcgc cgcgagcgca gatagcgcca ggcaaccatg
     6961 cgctcgaaca ccgagaacgg cccggccgcg gccgcgttct tcgtcgccgt cacgtcactc
     7021 atgcggttgc accgaagcgt tgcagggcgc tcgcaatcgg cagcgtctcg cgctcacccg
     7081 ttctgcggtt cttgatctcg acctcgccgg cagccacgcc gcgcggcccg acgataaact
     7141 gccacggcag gccgatcagg tcggcggtgg cgaatttgcc gcccggccgc tggtccgt
//
LOCUS       DASYCS010000219        15297 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4545880,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000219 DASYCS010000000
VERSION     DASYCS010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 15297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15297
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4545880"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1921
                     /gene="pepN"
                     /locus_tag="VGM46_07005"
     CDS             <1..1921
                     /gene="pepN"
                     /locus_tag="VGM46_07005"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEY4192379.1"
                     /translation="WDEEAFGREYDLDVFNIVAVSDFNMGAMENKGLNIFNDKYVLAD
                     QETATDADFANIEAIIAHEYFHNWTGNRITCRDWFQLCLKEGLTVFRDHEFSADQRSR
                     AVKRIAEVRTLRAHQFPEDQGPLAHPVRPRRYREINNFYTATVYEKGSEVVRMIRTML
                     GVETFRQGMDLYFLRHDGEAATIEDFIKVFEDVSGRDLSQFALWYHQAGTPNVTVSTS
                     HNPAAREFTLEIEQSVPPTPSESRKRLMHIPLAFGLVGADGKDLPYAAVEGATVENGV
                     IHIRKRRHTVRFSGVTQRPAVSLNRGFSAPLTLSVEQSMADKLFLAGNDSDPFSRWQA
                     FNTLLTDALIAAFRQILGGKEPHFADELIGLAGGIADDQTLEEAYRALALTLPGEADI
                     ARDIGRNIDPDAIFSSRKQLATAIAEQNQPLFARLHAQLHDSRGFNPDAAGAGRRALR
                     NTLLDYLSLLPGGAQLALSHFNSATNMTDRAAALTVLAHRHHGSAEAVEALAAFETRY
                     AGDPLVMDKWFQIQASVPGPQAVDSVRALTGHPAFSMTNPNRLRSLVGTFSGANQTGF
                     HRADGEGYRLFTETVLDVEKRNPQMAARLATALRSWRSLEPVRQAKAREALLAMANTE
                     NLSADLRDIVERTLA"
     gene            2116..4428
                     /locus_tag="VGM46_07010"
     CDS             2116..4428
                     /locus_tag="VGM46_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006331078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY4192380.1"
                     /translation="MAKADAWGAPGGMRFERRKAPGKARLIGNMRLIAEPAYRKLLAA
                     EPLLRKLIPALIVVFLIVVAALRFLSLMNWQDEIERNAKAVLMLAAGELVNADQASAG
                     GAQALLDRTAREGAVSRANVLAVTDSGFKVVAITGQSSDWLGRPLDRIVSGGQPLFLF
                     GDRAGVMKVRIDGQDWYAAVNLKEGNAGAVAAFIPSDAVFAEWRNTASLDVTMFVLTA
                     SVLMVILYAYFSQVARAEAADRFYIEAHERIDMALVRGRCGLWDWDMARGKMFWSRSM
                     YDMLGYEPCEAMLSFGEVDEIIHPEDGDLFDLAKRIVAREIDHIDQVFRMRHADGQWV
                     WMRARAQVVDPDAPEVQLIGIAVDVTEQRHLALRSEAADLRLRTAIENINESFVLWDS
                     SKNLIMCNSKYQQDNGLSDRDVMPGTARSVLEEKMAAFASERRLANANGPGGGVTYER
                     QLADGRWLQVNELRTRDSGMVSVGSDITQIKQHQEKLVDSERRLMATIHDLSLARRSE
                     EERSRELVELNRKYMKEKERAEAANQAKSEFLANMSHELRTPLNAIIGFSELMEQALF
                     GPLGSDRYEEYATDINSSGKYLLGVINDILDMSKIEAGQFSMDSEEIDLCPLIRETVR
                     VVSLQAAEKSITVETSIADAMTLVADRRAIKQIVINLLSNAVKFTGQGGHIMVRARNA
                     SGALTLTIEDNGCGIPKSALGKLGRPFEQVQNQFSKNHAGSGLGLAISRSLAELHGGA
                     LKIRSTEGAGTIVSVRIPIRKPSRSLKVAA"
     gene            complement(4411..7386)
                     /locus_tag="VGM46_07015"
     CDS             complement(4411..7386)
                     /locus_tag="VGM46_07015"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamine synthetase]
                     adenylyltransferase/[glutamine
                     synthetase]-adenylyl-L-tyrosine phosphorylase"
                     /protein_id="HEY4192381.1"
                     /translation="MAAASVGQSGWHLDPVTKLKPLDDARAARELADLAGVAMEEGLP
                     RLAKLLGGKGKASAFLAAAFDCSPFLRDLARRRPQMLDALFDGSVEARLDAILSGIAK
                     LADGEGVTEAVIMMELRSRKAEAHFLIALADLAGLAETAVTVRRLSALAEACVRSAVD
                     FLLRDAHRQGKLKLAELDRPAEKSGWILLGMGKLGACELNFSSDIDLVVFFDPQAPAI
                     IDPLDATDMFSRLTRRLVRILHDRTEHGYVFRTDLRLRPDPGSTPLAIPVEAALNYYE
                     GRGQNWERAAMIKARPVAGDLVAGAAFLKELQPYIWRKYMDYAAIADVHSIKRQIHAH
                     KGHGEIAVKGHNVKLGRGGIREIEFFVQTQQLIAGGRFPELRGRETVPMLGELAARGW
                     ITAEARDTLARQYWFLRRVEHAIQMVADEQTHMLPDDDEGLKRIALMLGFADAKTFSA
                     EFREALHEVERHYAALFETAPELSAGIGNLVFTGDVDDPDTLQTLNGLGFQRASDICR
                     VIRSWHFGRYRVTRSAEARERLTELTPALLQAFGKTRRADEALMRFDELLAGLPAGVQ
                     LFSLLQSNPALLRLLATIMGAAPRLAAIITRQPHVFDGLLDPGLLKDLPDRDYLSVRL
                     AAFLEGDRLYEEKLDRLRIFASEQKFLIGVRLLAGAIDPARAGRAFSDLADLTVGAAL
                     DAVMAEFAVRHGHIAGGKVALLGMGKLGSRELTAGSDVDLILLYDHEPEAEESDGDKP
                     LAPSQYFSRLTQRLIAAVSAPTAEGVLYELDLRLRPSGNKGPVATHIDAFSKYQRTEA
                     WTWEHMALSRARTIAGDAVLRKAIENEVAAVLALPGDDKKIMADAFEMRATIDEEKPP
                     RDLWDIKLIPGGLIDLEFIAQVAVITGKIEGAERTTATSQILARLAPDFADAQTRREL
                     GDAYALYLALTQMMRLCLTGDFDRDDVPPGLSDLLLAVTDLPDFSVLEAHLKDTSKLV
                     RKHFNGLLAAPGKKSGRHL"
     gene            complement(7386..8840)
                     /locus_tag="VGM46_07020"
     CDS             complement(7386..8840)
                     /locus_tag="VGM46_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019857153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY4192382.1"
                     /translation="MALSVPAIMKTTAARLSALYLLLFALCAVLLVFYMTSLSARMLT
                     GQTQDTINDEVLGLARAYQRGGLPTLVRVVEQRSRQPGANLYLIAEASGQILTGNVQS
                     LEPGVLETEGWTTSPFSYQRFGEGELDRQRNAAPDSGAARAEGETGHNAIALVLRLPN
                     QMIMLVGRDLGEPERFRAVIRRALMLALGMMGLGGILIWFFVGRAALKRIDSVSEASR
                     RIMGGDLAGRLPVTGAGDEFDRLSENLNTMLARIAALNEGLKQVSDNIAHDLKTPLTR
                     LRNRAEAALSGKHKVADYRGALERTIAESDHLIKTFNAILMISRLEAGYSSESTVRVD
                     LAAAVSDVVELYEPVAEEAGVTLEADIPEPMAVDGNRELIGQALSNVVDNAIKYSTDA
                     TTAPAVRVSLERKDGHVVLTVADNGQGIPDDTDRARVTERFVRLEKSRSQPGSGLGLS
                     LANAIMKFHGGRLDLVSGNPGLSVIMSFPERTDH"
     gene            complement(8847..9521)
                     /locus_tag="VGM46_07025"
     CDS             complement(8847..9521)
                     /locus_tag="VGM46_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010915052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY4192383.1"
                     /translation="MKILVMEDDREAADYLQKAFTEAGHTTHVAGDGETGFALADAGD
                     YDVMVVDRMMPRRDGLSVIAGLRSRGNTTPVLILSALGEVDDRVTGLRAGGDDYLTKP
                     YAFSELLARVEVLNRRASAKESETVYRVGDLELDRLSHSVKRMGQDITLQPREFRLLE
                     YLMRHAGQVVTRTMLLENVWDYHFDPQTNVIDVHVSRLRGKIEKGFDKPILHTVRGAG
                     YMLKGA"
     gene            complement(9718..11259)
                     /locus_tag="VGM46_07030"
     CDS             complement(9718..11259)
                     /locus_tag="VGM46_07030"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008839537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HEY4192384.1"
                     /translation="MNDAPNSYSRLPKRLLATVASVAIAGAIGFGAVTTGTAPVLADA
                     VHVQAPQVPSFADVVERVSPAVVSVRVKAKVQPTADDGSDMFQGGPDGMPDNPQLREF
                     FKQFRGFGDQNGHDQDGKRHNRSHRDDKPRPVAQGSGFFISEDGYLVTNNHVVDEGTA
                     FTVVMSDGKELDAKLVGTDPRTDLAVLKVDSDQKFTYVAFGDDAKARVGDWVVAVGNP
                     FGLGGTVTAGIISARGRDIGAGPYDDFIQIDASVNRGNSGGPTFNLNGEVIGINTAIF
                     SPSGGNVGIAFDIPASTAKEVVASLMKSGSVQRGWLGVEIQPVTSEIAESLGLKSEKG
                     ALVASAQDDGPAKKAGVVAGDVITQVDGKDVDSPKELARLIAGEAPGKAVELGIWRDG
                     KADTVKFELGKLPNADKQASADEQQEEPAKPDTLADLGLTVTKSDDGKGLVVTDVDPD
                     SSASDRGIQAGDIIVAVNSTEVNDTQQVSKAIADAAKAGRKSVLVQLTHDKNNRFVAL
                     PVAGS"
     gene            complement(11390..11854)
                     /locus_tag="VGM46_07035"
     CDS             complement(11390..11854)
                     /locus_tag="VGM46_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HEY4192385.1"
                     /translation="MNARKALVWLVLLTALSFSGSAFAVKPNEMLANPALEARARTIS
                     ESLRCLVCQNQSIDESDADLARDLRVLVRERITAGDSNAQVIDYVVSRYGEFVLLKPR
                     LSARTLLLWGTPIILLLAGGLAMFIAGRRRRAETPALTSEEQARLDAILRQG"
     gene            complement(11851..13842)
                     /locus_tag="VGM46_07040"
     CDS             complement(11851..13842)
                     /locus_tag="VGM46_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006331064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HEY4192386.1"
                     /translation="MVEIGHFALVLAFALALVQTIVPLIGARVGNQRMMAVAAPVAVT
                     GFAMTALSFAALATAYAVSDFSLQTVWENSQSLQPLIFKITGAWGNHEGSMLLWVLIL
                     TFFGALVALFGANLPATLKANVLAVQGLIGAAFFLFILITSNPFTRLNPAPIEGRDLN
                     PILQDFGLAVHPPLLYLGYVGFSVCFSFSVAALIDGRIDASWARWVRPWTLVAWMFLT
                     GGIAVGSYWAYYELGWGGFWFWDPVENASFMPWLSGTALLHSAIVMEKRSALKIWTLL
                     LAILTFSLSLLGTFLVRSGVLTSVHAFATDPTRGIFILCILIFFIGGSLALFAWRAST
                     LTAGGLFHPISREGALVLNNLFLTTAAATVLIGTLYPLLLEAVSSDKISVGPPFFNLT
                     FGPLMVPLLVMVPFGPLLAWKRGDIAAVAQRLMAAFAAALLASIITAFFIDGASAFAI
                     VGVGLAFWLIFGALTDLAVKSGFGNVAVSAMFRRLVGLPRSMFGTAFAHMGLGLTVLG
                     LVGTLSFGTEKILSMHTGETVELSGHTVRFAGLFPDKGPNYTEQRARFELLGVDGKTI
                     GAIDTAKRFYPVRQMTTTESGIKTIALSQLYISLGDEGTDGSVVVRLWWKPLVTLIWL
                     GALVMMAGAAASLMDRRLRVGAPSRRLAKSNATPAASAP"
     gene            complement(13861..14301)
                     /gene="ccmE"
                     /locus_tag="VGM46_07045"
     CDS             complement(13861..14301)
                     /gene="ccmE"
                     /locus_tag="VGM46_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006331062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEY4192387.1"
                     /translation="MTRKQKRLSVIIGGLAFLAAATGLTFYALGQKASYFYMPGDLAS
                     ATVQPRQRIRLGGLVEKGTIQRGQGATVAFSVTDTQKSVKVTYTGILPDLFREEQGVI
                     TEGSFGPNGVFVADSVLAKHDERYMPKEVADGLKAKGVWQESKK"
     gene            complement(14305..>15297)
                     /gene="ccmI"
                     /locus_tag="VGM46_07050"
     CDS             complement(14305..>15297)
                     /gene="ccmI"
                     /locus_tag="VGM46_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019857147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HEY4192388.1"
                     /translation="LDRDVARGMIHASQADQARAEIARRILRLDNAPSQPATAGSSRA
                     MRWVAMAAVLAVPLVSWGVYAKLGSPDLPSQPLAERLAKNPADSSVDELVARAEAHLA
                     TNPSDGRGWDVVAPIYLRMQRFPDAINAYRNAIRLDGDSAARQAGLGEAIANAAGGVV
                     STDAQAAFEAALKLDPANAKAGFYLALSMSQEGRNGEAMAQLQAVLAKLPQESPWRAA
                     IQQALADLGDRKAAGNQAAGPSRQQVDAASSMSQQDRSALIETMVAGLDEKLRQNPRD
                     AEGWNRLVRSYLVLGKTAQARDALNRGVAAFGAGSDEAKGLVAFAASLGLAATE"
BASE COUNT         2922 a         4795 c         4864 g         2716 t
ORIGIN      
        1 atgggacgag gaggccttcg gccgcgaata tgacctcgat gtcttcaaca tcgtcgccgt
       61 gtcggatttc aacatgggcg cgatggagaa caagggcctc aacatcttca acgacaaata
      121 tgtgctggcc gaccaggaaa ccgcgacgga cgccgatttc gccaatatcg aagcgatcat
      181 cgcgcatgaa tatttccaca attggacagg caacagaatc acttgccgcg actggttcca
      241 gctttgcctg aaggaagggc tgacggtttt ccgcgaccat gagttttccg ccgaccagcg
      301 ctcgcgcgcg gtcaaacgca ttgccgaggt acgcacgctg cgcgcgcacc agttccccga
      361 ggaccaggga ccgctggcgc atccggtgcg gcctcgccgc taccgcgaga tcaataattt
      421 ctatacggcg accgtctacg aaaagggttc cgaggtggtg cggatgatac gcaccatgct
      481 gggcgtcgaa acgttccggc agggcatgga cctctatttc ctgcgccatg acggcgaggc
      541 cgccaccatc gaggacttca tcaaggtctt cgaggacgta tccggccgcg acctgtcgca
      601 gttcgcgctg tggtatcatc aggccggaac gcccaacgtc accgtcagca cctcgcataa
      661 tccggcggca cgggagttta cgctggaaat agagcagtcg gtgccgccga cgccgtccga
      721 gagccgcaaa cggctgatgc acatcccgct cgccttcggg ctggtcggtg cggacggcaa
      781 ggatcttccc tacgccgccg tcgagggagc gacggtggag aacggcgtca tccacatccg
      841 caagcgccgc catacggtgc gtttttccgg cgtcacgcag cggcctgcgg tttcgctgaa
      901 ccggggtttt tcggctccgc tgacgctttc ggtcgaacag agcatggccg acaagctgtt
      961 ccttgccggc aatgacagcg accccttctc gcgctggcag gctttcaaca cgctgttgac
     1021 cgatgcgctg atcgcggcat tccgccagat acttggcggc aaggagccac acttcgccga
     1081 cgagctgatc gggctggccg gcggcatagc cgacgaccag acgctggagg aagcctaccg
     1141 cgcgctggcg ctgacacttc cgggcgaggc cgacattgcc cgcgacatcg gcaggaacat
     1201 cgacccggac gccatctttt ccagccgcaa gcaactcgcc accgcgatcg ccgagcaaaa
     1261 ccagccgctg tttgcccgcc ttcatgccca gttgcacgac agtcgcgggt tcaaccccga
     1321 tgcggccggt gctggccggc gggcgctgcg caacacgctg ctggattatc tctcgttgct
     1381 gccgggcggc gcgcagctgg cgctgtctca cttcaatagc gcaaccaaca tgaccgaccg
     1441 tgcggcggcg ttgacggtcc tggcgcaccg ccatcatgga tcggcggaag cggtcgaggc
     1501 gcttgcggct tttgagacgc gctacgccgg cgatccgctc gtcatggaca agtggttcca
     1561 gatccaggcc agcgtgccgg gaccgcaggc ggtcgacagc gttcgcgcgc tgaccggcca
     1621 tccggccttt tcaatgacca atccaaaccg gctgcgttcg ctggtcggca cgttttcggg
     1681 cgccaaccag accggcttcc atcgcgcgga cggcgagggt taccggctgt tcaccgaaac
     1741 ggtgctcgac gtcgaaaaac gcaatccgca gatggccgca agactggcga ccgcgcttcg
     1801 ttcatggcgc tcgctggagc cggtgcgcca ggcaaaggcg cgcgaggcgc tgcttgccat
     1861 ggcgaatacc gaaaatctgt cagccgattt gcgcgacatc gtcgagcgca cgctggcata
     1921 atctggggtc aagcggcccc ggtttgcgct gcccggcagg tcaatatggg ctaaatcggc
     1981 aatttttagt ctatttttaa tcttttttcg ccgcgcctat ggacaaggcg aatcaccttt
     2041 gattctttac tgacgattcg ggcgtgcggc gtaaccgggt catagggaca gcattaaatc
     2101 ggggagagcc atttcatggc caaggcggac gcgtggggcg cgcccggggg gatgcgattt
     2161 gagcggcgca aggcgccggg caaggcgcgc ctgatcggca acatgcggct tatcgccgaa
     2221 ccggcctatc gcaaactgct cgcggcggaa cccctgttgc gcaagcttat ccctgccctg
     2281 atcgtcgtct tcctgatcgt ggtggcggcg ctgcgtttcc tgtctctaat gaattggcaa
     2341 gacgagatcg aacgcaatgc caaggcggtt ctcatgctcg ccgccggcga attggtgaat
     2401 gccgatcaag cgtcggccgg tggagcgcag gcgcttctcg accgcaccgc gcgcgaaggc
     2461 gccgtcagcc gcgccaacgt actggccgtc accgacagcg gcttcaaggt cgtcgccatc
     2521 acggggcagt catccgactg gctcggccgg ccgctcgacc gcatcgtttc gggcggacag
     2581 ccgcttttcc ttttcggcga ccgcgccggc gtcatgaagg tgaggatcga cgggcaggac
     2641 tggtacgcgg cggtaaacct gaaggaaggc aatgccggcg cggtagctgc cttcattccc
     2701 agcgacgcgg tatttgcgga atggcgcaat accgcctcgc tcgacgtcac catgttcgtg
     2761 ctgaccgcca gcgtgctgat ggtcatcctc tacgcttatt tcagccaggt ggcgcgcgca
     2821 gaggcggcgg accgcttcta catcgaagcc catgaacgca tcgacatggc gctggtgcgg
     2881 ggccgctgcg gcctgtggga ctgggacatg gcgcgcggca agatgttctg gtcgcgctca
     2941 atgtacgaca tgctcggcta cgagccatgc gaggcgatgc tttcattcgg cgaggtcgat
     3001 gaaatcatcc atcccgagga tggcgacctg ttcgacctcg ccaagcggat cgtcgcgcgc
     3061 gaaatcgatc acatcgacca ggtattccgg atgcgccacg cggacggaca atgggtgtgg
     3121 atgcgcgccc gcgcccaggt cgtcgacccg gacgcgccgg aggtccagct gatcggcatc
     3181 gccgtcgacg ttaccgagca acgccatctg gcgctgcgct cggaagcggc ggacctcagg
     3241 ctgcgcacgg cgatcgaaaa catcaacgaa tccttcgtgc tgtgggattc gtccaagaac
     3301 ctcatcatgt gcaattccaa gtaccagcag gataacggcc tgtctgaccg cgacgtgatg
     3361 cccggcaccg cccgctcggt tctggaagaa aagatggccg cattcgcctc cgagcgccgg
     3421 ctggccaacg caaacgggcc gggcggcggc gtgacctacg aacgccagct ggcggacggc
     3481 cgctggctac aggtcaacga actgcgcacg agggacagcg gcatggtttc cgtcggttcc
     3541 gacatcacgc agatcaagca gcatcaggag aagctggtgg acagcgagcg ccgcctgatg
     3601 gcgaccatcc acgatctcag cttggcgcgc cgttcagaag aggagcgttc gcgcgaactg
     3661 gtggaactaa accggaaata catgaaggaa aaggagcgcg ccgaggcggc caaccaggcc
     3721 aaatccgaat tcctcgccaa catgtcgcat gagctgcgta cgccgctcaa cgccatcatc
     3781 ggcttctcgg agctgatgga gcaggcgctt ttcggcccgc tcggctcgga tcgctatgag
     3841 gaatatgcca ccgacatcaa ttcgagcggc aaatatctgc tgggcgtcat caacgatatt
     3901 ctcgacatgt cgaagatcga ggccggacag ttctccatgg acagcgagga gatcgatctt
     3961 tgcccgctga tccgcgaaac ggttcgcgtc gtttcgttgc aggcggcgga gaaatcgatc
     4021 accgtcgaga cgagtatcgc cgatgccatg acgctggttg cggaccggcg cgcgatcaag
     4081 cagatcgtca tcaaccttct gtcgaacgcg gtgaagttca ccggccaggg cggacacatc
     4141 atggtgcggg cgcgcaacgc ttccggcgcg ttgacgctga ccatcgagga caatggctgc
     4201 ggtatcccga aaagcgcgct gggcaagctt ggacggccgt tcgaacaggt gcaaaaccag
     4261 ttctccaaga accatgccgg ctcggggctt ggcctggcca tttcgcgctc gctggcggag
     4321 ctgcatggcg gcgcgttgaa aatccgctcg accgagggag ccggcacgat cgtctcggtg
     4381 cgtatcccga tccgcaagcc ttcgcgcagc ctaaaggtgg cggcctgatt tcttcccggg
     4441 tgcggcgagc agcccgttga aatgtttgcg gaccagcttt gacgtgtcct tcaaatgggc
     4501 ttcgagtacg ctgaaatccg gcaggtcggt gacggccagc agcaggtcgg acaggccggg
     4561 cggaacgtcg tcgcggtcga agtcgccggt caggcaaagc cgcatcatct gggtcagcgc
     4621 cagataaagg gcataggcgt cgccaagttc gcgccgtgtt tgggcatccg cgaaatccgg
     4681 cgccaggcgt gccaatatct gcgatgtagc cgtcgtccgt tcggcccctt cgatctttcc
     4741 ggttatgacg gcgacctgcg cgatgaattc cagatcgatc agcccgccgg gaataagctt
     4801 gatatcccac agatcgcgcg gcggcttttc ctcgtcgatc gtggcgcgca tctcgaaagc
     4861 atcggccatg atctttttgt cgtcgcccgg cagggcaagc acggcggcaa cctcgttttc
     4921 gatggccttg cgcaggacgg catcgccggc gatggtgcgg gcgcgcgaca gcgccatatg
     4981 ctcccacgtc caggcttcgg tgcgctgata tttgctgaag gcatcgatat gcgtggccac
     5041 cggtcccttg ttgccggaag ggcgcaggcg caggtcgagt tcgtaaagaa cgccctcggc
     5101 ggtcggcgcg gagacggccg cgatgaggcg ctgggtcagg cggctgaaat actgggaagg
     5161 agccaacggc ttgtcgccgt ccgactcttc ggcctccggc tcgtgatcgt agagcaggat
     5221 caggtcgaca tcggaaccgg cggtcaattc gcggctgccg agcttgccca tgcccaacag
     5281 ggcaaccttg ccaccggcaa tatggccatg cctgacggcg aattccgcca ttacggcatc
     5341 aagggcggcg ccgaccgtca ggtcggcaag gtcggaaaac gccctgcccg cccgcgccgg
     5401 gtcgattgcg ccggccagca accgcacgcc gatcaggaat ttctgttccg aagcgaagat
     5461 gcgcagccgg tcgagttttt cctcataaag ccggtcgccc tccaggaaag cggcgagccg
     5521 caccgaaaga tagtcgcggt ccggcaaatc cttgagcagg cctggatcga gcaggccatc
     5581 gaacacatgc ggctggcggg tgatgatcgc agcaaggcgg ggggcagcgc ccatgatcgt
     5641 cgccaacagc ctgagcaatg ccggattgga ctggagcagc gagaaaagct ggacgccggc
     5701 gggaagcccg gccagcaatt cgtcgaaccg catcaacgct tcgtcggcgc gacgcgtctt
     5761 gccgaaggct tgaagcaggg ccggagtgag ttcggtcagc cgttcgcggg cttccgccga
     5821 gcgggtgacc cggtagcggc cgaaatgcca gctgcggatg acgcggcaga tatcgcttgc
     5881 cctctggaag cccaggccat tcagggtttg cagcgtatcc ggatcgtcga catcgccggt
     5941 gaagaccaga ttgccgatgc ctgcggaaag ctccggcgcg gtttcgaaca gggccgcata
     6001 gtgccgctcc acctcgtgga gcgcctccct gaattcggcg gaaaaggttt tggcatccgc
     6061 gaacccaagc ataagggcga tgcgtttcag cccctcgtca tcgtcgggca gcatgtgggt
     6121 ctgttcgtcg gcgaccatct ggatggcgtg ttcgacacgg cgcaggaacc aatattggcg
     6181 cgccagcgtg tcgcgcgctt cggccgttat ccagccgcgc gcggcaagtt cgcccagcat
     6241 cggcacggtt tcgcggccac gcagttccgg gaaccggccg ccggcgataa gctgctgggt
     6301 ctggacgaaa aattcgattt cgcggatgcc gccgcgaccc agcttgacgt tatggccctt
     6361 caccgcgatt tcgccatggc ccttgtgggc gtgaatctgg cgcttgatcg aatgcacgtc
     6421 ggcgatggcc gcatagtcca tatatttgcg ccagatatag ggctgaagtt ccttcaggaa
     6481 ggctgcgccg gccaccaggt ctccggctac cgggcgtgcc ttgatcatgg cggcgcgctc
     6541 ccagttctgg ccacgccctt catagtagtt gagcgccgcc tcgaccggga tcgctaaggg
     6601 cgtggacccc ggatcggggc ggaggcgcaa atccgtgcgg aacacgtaac catgttcggt
     6661 gcggtcatgc aggatacgga ccagccgccg ggtcagcctc gaaaacatat cggtggcatc
     6721 aagcgggtcg atgattgcgg gggcctgggg atcgaagaag actacgaggt cgatgtcgga
     6781 cgagaaattc agctcgcagg cgccaagctt gcccatgcca agcaatatcc agcccgactt
     6841 ttccgctggc ctgtccagtt cggcaagctt gagcttgccc tgccgatgcg cgtcgcggag
     6901 cagaaaatcg accgcagagc gcacgcacgc ctcggcaagc gcgctcaacc gccgcacggt
     6961 gacggcggtt tcggccaggc cggccagatc ggccagcgcg atcaggaaat gcgcctcggc
     7021 cttgcggctg cgcagttcca tcatgatgac ggcttccgtg acgccttccc catccgcgag
     7081 tttggcgatg ccggagagga tggcgtcgag cctcgcttcg accgatccgt cgaaaagagc
     7141 gtcgagcatc tgcggacggc ggcgggcgag atcgcgaagg aagggcgagc aatcgaacgc
     7201 ggcggcaaga aaagcggaag ccttgccctt gccacccagc agtttcgcca gccggggaag
     7261 gccctcctcc atggccacgc cggcgaggtc tgccaattcg cgcgccgcgc gggcatcgtc
     7321 caacggcttc agctttgtta cgggatcaag atgccaacca ctttgcccaa cgcttgccgc
     7381 cgccatcaat gatccgttcg ttcggggaaa ctcatgatga cggataatcc gggattgccc
     7441 gacaccaggt caagcctgcc gccgtgaaac ttcatgattg cgttggcgag gctcaagccc
     7501 aaccctgaac ccggctgcga gcggcttttt tccagccgaa cgaagcgttc ggtgacacgg
     7561 gcacggtcgg tatcgtcggg gatgccctgc ccattgtcgg ccaccgtcag cacgacatgg
     7621 ccgtccttgc gttcgaggga aacgcgcacc gccggtgctg tggtagcgtc ggtcgaatat
     7681 tttatcgcgt tgtccacgac gttggacagc gcctggccga tcagttcacg gttgccgtcg
     7741 acggccatcg gctcggggat atcggcttcc agcgtgacgc ccgcttcctc ggccaccggc
     7801 tcatacagtt cgacgacatc gctgacggcc gccgccagat cgaccctgac cgtgctttcc
     7861 gacgaataac ccgcctccag ccgcgagatc atcaaaatgg cgttgaacgt cttgatgaga
     7921 tggtccgatt ccgcaatcgt gcgctcaagc gccccgcggt agtcggcgac cttgtgcttg
     7981 ccggaaaggg ctgcttcggc gcgattgcgc agccgggtca gcggggtctt caggtcgtgg
     8041 gcgatattgt cggagacctg cttcagccct tcgttgagcg ccgcgatcct ggccagcatg
     8101 gtgttcagat tttcggagag gcggtcgaat tcgtcaccgg cgccggtcac cggaaggcgg
     8161 cccgccagat cgccgcccat gatgcggcgg ctggcttcgg aaacgctgtc gatgcgcttc
     8221 aacgcggcac gcccgacaaa gaaccagatc aggatgccgc caaggcccat catgccgagc
     8281 gccagcatca acgcgcggcg aatgacggcg cggaaacgct ccggctcgcc gaggtcgcgc
     8341 ccgaccagca tgatcatctg gttaggcagc ctcagcacca gcgcgatggc gttatggccc
     8401 gtttcgccct ccgcccgcgc agcgccgctg tcgggcgcgg cattgcgctg cctgtccagt
     8461 tcgccttcgc cgaaacgctg gtaggagaac gggctggtag tccagccttc ggtttccagc
     8521 acgcccggct ccaggctctg cacattgccg gtaaggattt ggccgctggc ttcggcgatc
     8581 agatagagat tggcgccggg ttggcgcgac cgctgctcga ccacccgcac aagcgtcggc
     8641 agcccgccgc gctggtaggc acgggcgagg cccaacactt cgtcgttgat ggtgtcctgc
     8701 gtttgccccg tcagcatgcg ggccgacagc gaggtcatgt agaaaaccag cagaaccgcg
     8761 caaagcgcaa acagcagcag gtaaagcgcg gaaagccgcg ccgccgtcgt cttcatgatg
     8821 gcgggaacgg acagcgccat tccgcgtcag gcgcccttca gcatataacc ggcaccccgg
     8881 accgtgtgca ggatgggctt gtcgaagccc ttctcgatct tgccgcgcag ccgcgatacg
     8941 tgaacgtcga tgacgttggt ctgcgggtcg aaatgataat cccagacatt ttcgagcagc
     9001 atggtgcggg tcaccacctg cccggcatgg cgcatcagat attccagcaa ccggaattcg
     9061 cgcggctgaa gcgttatgtc ctgccccatg cgcttgaccg aatgggagag ccggtcaagc
     9121 tcaaggtcgc cgacgcggta gacggtttcg ctttccttgg cgctcgcgcg gcggttcagc
     9181 acctcgacgc gcgcaagaag ctccgaaaag gcgtagggct tggtcaggta gtcgtcgccg
     9241 ccggcgcgca ggccggtcac gcggtcgtcc acctcgccca gcgccgacaa gataagcacg
     9301 ggcgtggtat tgccgcgcga gcgcaatccc gcgatgacgg aaaggccatc gcggcgcggc
     9361 atcatgcggt ccaccaccat gacgtcgtag tcgcccgcat cggccagcgc gaagcccgtc
     9421 tcgccgtcac cggcgacatg cgtggtatgc ccggcttcgg tgaaagcctt ctggagatag
     9481 tcggcagcct cgcggtcatc ttccatgacg agaatcttca tggcgtcgtt ataacccatt
     9541 ccgaccgatc gagatgccga catctgaatt gacctcaacg tttatgcggc agcgggcgat
     9601 gggacccgct gccgccgaaa ccgcgcgcta ttacgattta ctgaagcgtt cggttccgtc
     9661 tttcccgcgc ggcttttcgg gggtgtttga aacgccgcgc gggaaaccgg ccgtcgctca
     9721 gcttccggca accggcagag cgacaaagcg gttgtttttg tcatgcgtca gctggaccag
     9781 caccgacttg cggccggcct tggcggcatc ggcaatcgcc ttggaaacct gctgggtgtc
     9841 gttcacctcg gtcgagttga cggcgacaat gatgtcgcct gcctggatgc cacggtccga
     9901 tgccgagctg tcgggatcga catcggtaac gaccagcccc ttgccgtcat cggatttggt
     9961 gacggtcagg ccgagatcgg caagcgtatc gggcttggcc ggttcttcct gctgctcatc
    10021 cgccgacgcc tgcttgtcgg cgttcggcaa tttgccgagt tcgaacttca ccgtatcggc
    10081 cttgccgtcg cgccagatgc caagctcgac tgcctttccg ggcgcttcgc cggcgatgag
    10141 gcgggcgagt tccttgggcg aatcgacatc cttgccatcg acctgcgtga tgacatcgcc
    10201 ggcgaccacg ccagccttct tggccggacc gtcatcctgc gcgctggcga caagcgcgcc
    10261 cttttcggac ttcaggccga gcgattcggc aatttcggac gtgaccggct ggatttcgac
    10321 accgagccag ccgcgctgca ccgagccgct tttcatcagc gatgcgacaa cttccttggc
    10381 ggtcgatgcc ggaatgtcga aggcgatgcc gacattgccg cccgatggcg agaagatcgc
    10441 cgtattgatg ccgatgactt cgccgttcag gttgaaggtc gggccgccgg aattgccgcg
    10501 gttgaccgac gcgtcgatct ggatgaagtc gtcatagggg ccagcgccga tatcgcggcc
    10561 gcgtgcggag atgatgccag cggttaccgt tccgccgagg ccgaacggat tgcccacagc
    10621 gaccacccag tcgccgacgc gggccttggc gtcgtcgccg aaggcgacat aggtaaactt
    10681 ctggtcgcta tcgaccttca gcacggcaag atcggtgcgc ggatcggtgc cgactagttt
    10741 ggcgtccagt tccttgccgt cactcatgac gaccgtgaaa gccgtacctt cgtcgacgac
    10801 gtggttgttg gtaaccagat aaccgtcttc ggagatgaag aaaccggacc cttgcgcgac
    10861 gggacggggc ttgtcgtcgc ggtggctgcg gttatggcgc ttgccgtcct ggtcgtgacc
    10921 gttctggtcg ccaaagccac ggaactgctt gaagaactcg cgcaattgcg gattgtcggg
    10981 catgccgtcc ggtccgccct ggaacatgtc cgagccgtca tcggcagtcg gctgcacctt
    11041 ggccttgacg cgcacgctga ccaccgccgg cgaaacgcgc tcgacgacat ccgcgaagct
    11101 tggaacctgc ggcgcctgca cgtgcacggc atcggccagc acgggggccg ttccggtggt
    11161 tacggcgccg aagccgatcg cgccggcgat agcgacggat gcgacggttg cgagcagacg
    11221 ttttgggagg cgggagtatg aattaggggc gtcgttcatg gtctctatcc ttctaccttg
    11281 ttcgcgggat gcgtggcctg caccctgtga aggtaagaga taaagcgcga gacattaccg
    11341 ggccatttcc ggggaatgaa actttggtaa tgttgcgaag ccggtcgctt cacccctggc
    11401 gcaaaatggc gtccagcctg gcctgctctt cgctggtgag cgcgggcgtt tcggcccgac
    11461 gcctgcgccc ggcgataaac atggccaacc cgccagccag cagcaggatg atcggggtgc
    11521 cccacaaaag cagggtgcga gcgctcaaac gcggcttgag cagcacgaac tcgccataac
    11581 gcgagacgac atagtcgatc acctgggcat tgctgtcgcc ggcggtgatc cgctcgcgca
    11641 ccagaacgcg caggtcgcgg gccagatcgg catccgactc atcgatcgac tggttttgac
    11701 agacgaggca gcgcaggctt tccgagatgg tgcgcgcccg cgcttcaagc gccgggttcg
    11761 ccagcatctc gttcggcttc acggcaaaag cgctgcccga gaaagacagg gccgtcaaca
    11821 aaacgagcca gacgagcgct tttctcgcat tcatggcgcg ctcgccgccg gcgtcgcatt
    11881 cgacttggcg agacggcgcg acggtgcgcc gacgcgcaag cgccgatcca tcagggaggc
    11941 tgcggcgccc gccatcatga ccagcgcacc cagccagatc agtgtgacca gcggcttcca
    12001 ccacaggcgc accaccacgg aaccatccgt tccttcatcg ccgagcgaaa tatagagctg
    12061 gctcaatgcg atcgtcttga tgccggattc cgtcgtggtc atctggcgga ccggatagaa
    12121 ccgcttggcg gtgtcgatgg cgccaatcgt cttgccgtcg acgccgagca attcgaagcg
    12181 ggcgcgctgt tcggtgtagt tcggaccttt atccgggaac agccccgcga aacgcaccgt
    12241 atggccagac aattccacgg tctcgcccgt atgcatggaa agtatctttt ccgtgccgaa
    12301 cgacagcgtg ccgacgaggc cgagaacggt caggcccaat cccatatgcg caaacgccgt
    12361 gccgaacatc gagcgcggca ggccgacaag acgccggaac atggccgaga ccgctacatt
    12421 gccgaagccc gacttgaccg ccagatcggt gagggcaccg aagatcaacc agaatgccag
    12481 cccgacgccg acgatggcaa aagccgaagc gccgtcaatg aagaacgcgg ttatgatgct
    12541 ggccagaaga gctgcggcga atgccgccat cagccgctgg gcgaccgctg cgatgtcgcc
    12601 gcgcttccag gcaagcagcg gcccgaacgg aaccatgaca agcagcggca ccatcaacgg
    12661 gccgaaggtc aggttgaaga atggcgggcc gaccgaaatc ttgtcggagg agaccgcctc
    12721 cagcagcaat ggataaagcg tgccgatcag gacggtcgcg gcggcggtcg tcaggaaaag
    12781 gttgttcagc accagcgccc cttcccgcga gatcggatgg aagaggccgc ccgccgtcag
    12841 cgtggaggcg cgccatgcga acagcgccag cgatccgcca atgaagaaaa tcaggatgca
    12901 gaggatgaat atgccccgcg ttggatcggt ggcgaaggca tggacggagg tcaaaacgcc
    12961 ggagcgcacc aggaaagtgc caagcagcga cagtgaaaat gtcaatatgg cgagcagcag
    13021 cgtccatatc ttcagcgccg agcgcttttc catgacgatg gccgagtgaa gaagtgcggt
    13081 tccagacaac catggcatga acgacgcgtt ttccaccggg tcccagaacc aaaagccgcc
    13141 ccagcccagt tcataataag cccagtacga cccgacggcg atgccgccgg tgaggaacat
    13201 ccaggcaacc agcgtccaag gccgcaccca acgcgcccag gaggcgtcga tgcggccatc
    13261 gattagcgcc gccacggaaa acgaaaagca gactgaaaag cctacatagc cgagatagag
    13321 cagcggagga tgcacggcga ggccgaaatc ctgaaggatg ggattgagat cgcgcccctc
    13381 gatcggcgcc gggttcagcc gtgtgaacgg gttcgacgtt atcaggatga acaggaagaa
    13441 ggcggccccg atcaagcctt gcactgccag cacgttggcc ttgagcgttg ccggcagatt
    13501 ggcgccgaac agcgcaacca gcgcgccgaa gaatgtcagg atcagcaccc aaagcagcat
    13561 ggagccttcg tgattgcccc aggcgccggt gatcttgaag atgagcggtt gcagcgactg
    13621 ggaattttcc cacaccgttt gcagcgaaaa atcagacacg gcataggccg tggccaatgc
    13681 cgcgaacgaa agcgcggtca tggcaaagcc ggtcacagcc accggcgcgg cgaccgccat
    13741 cattcgctgg tttcccacgc gtgcgccgat caatggaacg atcgtctgga caagcgccag
    13801 cgcgaaagcc agaaccaatg caaaatgtcc gatttcaacc atgacggaag cgcctgccgt
    13861 tcatttttta ctctcttgcc agacgccctt ggctttcagg ccatcggcca cttccttggg
    13921 catatagcgt tcgtcatgct tggccagaac gctgtcggca acgaatacgc cgtttggtcc
    13981 gaaagaacct tcggtgatga cgccctgctc ttcgcggaaa agatcgggca ggatgccggt
    14041 ataggtcacc ttgaccgatt tttgcgtgtc ggtcaccgaa aaggcgacgg tcgcgccttg
    14101 gccgcgctga atggtgccct tttcgaccag tccgccgagc cttatgcgct ggcggggttg
    14161 cacggtggcg cttgcgaggt cgcccggcat gtagaaatag gaagccttct ggccgagcgc
    14221 atagaatgtc agcccggtcg ccgcggccag gaaggccagc cccccgatga tcaccgacag
    14281 tcttttctgc ttgcgcgtca tcgcctactc cgtcgcggcc agaccgagcg aagcggcaaa
    14341 ggccaccagg cccttcgctt cgtcgctgcc ggctccaaac gcggcaacgc cacggttgag
    14401 agcatcgcgg gcctgggcag tcttgccaag caccagatag gagcgaacca gccggttcca
    14461 cccctcggcg tcacggggat tttgcctgag cttttcatcc aggccggcaa ccatggtttc
    14521 gatcaaggcg ctgcgatcct gctgcgacat ggaggatgcg gcatcgactt gctgcctgga
    14581 tgggccggct gcctgattgc cggccgcctt tcggtcgccc agatccgcaa gcgcctgctg
    14641 gatggcggcg cgccagggtg attcttgcgg caacttggca agcactgcct gcaactgcgc
    14701 catcgcttcg ccgttgcggc cttcctgcga catggaaagg gccaaataga agcctgcctt
    14761 ggcattggcg ggatcaagct tgagggcggc ctcgaacgcg gcctgagcat cggtcgaaac
    14821 gacgccgccc gcggcgttgg caatggcctc gcccaaccct gcctgacgcg cggcactgtc
    14881 gccgtcgagc cggatggcgt tgcgataagc gttgatggca tcgggaaagc gctgcatgcg
    14941 cagatagatc ggcgccacca catcccatcc cctgccgtcc gaaggattgg tggcgagatg
    15001 cgcttcggcg cgggccacca gttcgtccac cgaactatcg gccggatttt tcgccagcct
    15061 ttcagccagc ggttgcgacg gaagatcggg cgagcccagt ttcgcgtaga cgccccagct
    15121 gaccaacggg accgccagca cggcggccat cgccacccag cgcatggcgc gcgaggaccc
    15181 cgctgtcgcc ggctgtgacg gcgcattgtc cagccgcagg atacgccgtg cgatttcagc
    15241 gcgagcctga tcggcctgcg aggcgtggat catgccgcgg gcaacatcac ggtcgag
//
LOCUS       DASYCS010000220         5986 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1358592,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000220 DASYCS010000000
VERSION     DASYCS010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 5986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5986
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1358592"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VGM46_07055"
     CDS             <1..290
                     /locus_tag="VGM46_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEY4192389.1"
                     /translation="AIPAVKPAPSVDDAVDDTAEDKADSAPAGPSSDAKAAQGHIVHG
                     VTMRAAPKKGAAAMTTIPAKTAVQVLNCAQWCQVVYNGKRGWVYKSFVTRD"
     gene            732..2204
                     /gene="zwf"
                     /locus_tag="VGM46_07060"
     CDS             732..2204
                     /gene="zwf"
                     /locus_tag="VGM46_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013530236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEY4192390.1"
                     /translation="MTSQIIPVDPFDFIIFGGTGDLAERKLLPSLYYRQCDQQFSEPT
                     RIIGTSRSKMSDDEFRAFAKKAISEHVAKADIDAKELTKFVNRLSYVAADAISGAGFE
                     QLKKEIGDSDHIRAFYLAVSPALFGEISHKLKEHDMITPKSRIVVEKPIGRDLDSARA
                     LNDQIGSDFHESQIFRIDHYLGKETVQNLMALRFANALYEPLWNSAHIDHVQITVAET
                     VGLEDRVTYYDKAGALRDMVQNHIMQLVCLVAMEAPSSLDADALRDEKLKVLRALKRI
                     NGTEAPKHTVRGQYRAGASAGGPVEGYVEELGNASDTETFVAIKAEIANWRWAGVPFY
                     LRTGKRLASRVSEIVIEFKPIPHSIFEGEAAGPIFPNQLVIRLQPDEGVKQFIMIKDP
                     GPGGMRLRQIPLDMSFAQSFKGRSPDAYERLIMDVIRGNQTLFMRRDEVEAAWKWIDP
                     IQNAWANARQEVQGYTAGTWGPSASIALIERDGRTWHESN"
     gene            2204..2902
                     /gene="pgl"
                     /locus_tag="VGM46_07065"
     CDS             2204..2902
                     /gene="pgl"
                     /locus_tag="VGM46_07065"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEY4192391.1"
                     /translation="MATRNYHRHDFSTRDELAAALAACVGGILGDEIAAAQAASIAVS
                     GGSTPARFFEVLSNVELDWKSVNVTLIDERYVPDTEPRSNAGLVKANLLQNRAAGANF
                     LPLYQSGDAGADMKEASRFLSTFFNPPDVAILGMGNDGHTASFFPDAVELEKLLDPQG
                     THEIASVHAQSAGELRLTLTLPLIARARFIALHIEGEEKQAALEKALSPGSTLPIRRM
                     IDAAKTPVEIFWAP"
     gene            2927..4750
                     /gene="edd"
                     /locus_tag="VGM46_07070"
     CDS             2927..4750
                     /gene="edd"
                     /locus_tag="VGM46_07070"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018426561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HEY4192392.1"
                     /translation="MTARRDIEAITERIRERSRISRETYLGRIAEASGRAANRGMLAC
                     GNLAHGFAVCSPSEKIALSGDTVPNLGIVTSYNDMLSAHQPFETYPALIKEAAKEAGG
                     IAQVAGGVPAMCDGVTQGQPGMELSLFSRDVIAMAAAIGLSHNMFDAAAFLGVCDKIV
                     PGLAIAALSFGHLPAVFIPAGPMTSGLPNDEKARVRQLYAEGKVGRAELLAAEAKSYH
                     GPGTCTFYGTANSNQMLMEIMGLHMPGASFVNPGTRLRDALTREATRRALAITALGNA
                     YTPVGRMIDERSVVNGVVGLHATGGSTNHTIHLIAIAAAAGIKLTWQDISDLSEAVPL
                     LARIYPNGLADVNHFQAAGGMGFLIRELLDAGLLHEDVQTVWGEGLRPYAIEATLGAD
                     GSVERAPAPRQSGDEKVLAPYAKAFQPSGGLKVLSGNFGHAIIKTSAVKPERRLIEAP
                     ARVFDSQHALNDAFKAGSLTGDFIAVIRFQGPKANGMPELHKLTTVLGVLQDRGQKVA
                     LVTDGRMSGASGKVPAAIHVTPEALEGGPIGKVRDGDIVRLDAEMGTLEILVPAGDLA
                     LRPAATADLSANEFGFGRELFAGFRALVTRADQGAAAFGNA"
     gene            complement(4811..>5986)
                     /locus_tag="VGM46_07075"
     CDS             complement(4811..>5986)
                     /locus_tag="VGM46_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006329329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192393.1"
                     /translation="SAQDGKQVACLAQSTGGKYIEASDAKALQAALVESVAATPAAQA
                     TSAPSAQAAPETAAPSAAETARPEFNFIPTVTLAQDGAPITQGNAWEIYKAAANGARG
                     ASIATEYGAYQGNLEPGDYILVARDGEAKAEQPLKVEAGQVYRPNLVLNAGTLRLRPR
                     TSAGGDMAGGAAVTVTYPGADIPTTRYGETSLVVPAGEQKITVTLGSASVDDTVQLTA
                     GQTLEKDIVVGVGHVVANAYYVAGGEKADSSGVGFKVFKAEKTGDGTREQVGYGYGSD
                     SKFDLAAGDYVIQATVDLAVAEQPFTVKVGEERALKVAVDAGVLSIDAPGANRIEIFG
                     KDADGKRKSVGYAYDEKFQPAVLAGDYAIVVEKSGGGAPSEKTATVHAGERTEVTIQ"
BASE COUNT         1094 a         1935 c         1900 g         1057 t
ORIGIN      
        1 ccgccattcc cgccgtgaaa ccggcaccgt cggtggacga tgccgtagac gataccgccg
       61 aagacaaggc cgacagcgcg cctgccgggc catccagcga cgccaaggcg gcgcaaggcc
      121 atatcgtgca cggcgtcacc atgcgggccg ctcctaaaaa gggcgctgcg gcgatgacga
      181 cgatccccgc gaaaaccgcc gtgcaggtgt tgaactgcgc ccaatggtgc caggtcgtct
      241 ataatggcaa gcgcggctgg gtctataaaa gcttcgtcac ccgcgactga caaaacggcg
      301 cagcagttgc cgccggctgt ttcccgcctc gaccgtcccg ttgaagacca tttcttgttt
      361 caagcctatg gctttgcccg cttcgttcat atcggcgtca ccgggtgcag ctatcgatgc
      421 gggttggcgc cgggctggca agcctggcga tacccgctga agacgccatc gacacccatc
      481 ggccaacacc agacgcatct tgcgcaactg tcactgaaag gtgcgacgcc gtgctcgatt
      541 tcccctttgg cttcgacaac ggaccgcttt atgttgcggc ggcgacgaac agcggggatc
      601 gttgggcaca tgagcgctcc catgctggac cagcgacgcc gtgaatgctg tgacaccatg
      661 gcactggtca atctaaatcg attagattat agcaacccgg taaagattgc cggcgtcaga
      721 acggacagac aatgacaagc cagatcatcc ccgtggatcc tttcgacttc atcattttcg
      781 gcggcaccgg cgatcttgcg gaacgcaagc tgctgccgtc gctgtattat cgccagtgcg
      841 accagcagtt ttccgaaccg acgcgcatta tcggcacgtc gcgctcgaaa atgtccgacg
      901 atgaatttcg cgctttcgcc aagaaggcga tttccgagca tgtcgccaag gcggatatcg
      961 acgccaagga gctgacgaag ttcgtgaacc gcctcagcta tgtcgccgcc gacgccatca
     1021 gcggggccgg attcgagcaa ctcaaaaagg aaatcggcga cagcgaccac atccgcgcct
     1081 tctacctcgc ggtttcgccc gctttgttcg gtgaaatttc gcacaagctc aaagagcacg
     1141 acatgatcac gcccaagtcg cggatcgtgg tggaaaagcc gatcgggcgc gacctcgatt
     1201 cggcgcgggc gctcaacgac cagatcggca gcgactttca cgaaagccag atcttccgca
     1261 tcgatcatta tctcggcaag gagacggtgc aaaacctgat ggcgctgcgc tttgccaatg
     1321 cgctgtacga gcctttgtgg aactcggccc atatcgacca tgtgcagatc acggttgccg
     1381 aaaccgttgg gctggaggat cgcgtcacct attacgacaa ggcgggcgcg ctgcgcgaca
     1441 tggtgcagaa ccatatcatg cagctggtct gcctcgtcgc catggaagcg ccttcctcgc
     1501 tggatgccga tgctttgcgc gacgagaaat tgaaggtgct gcgagcactg aagcgtatca
     1561 acggcaccga agcgcccaag catacggtgc gcgggcaata ccgcgccggc gcttcggcgg
     1621 gcggcccggt ggaaggctat gtcgaggagt tgggcaatgc cagcgacacc gaaaccttcg
     1681 tcgccatcaa ggccgagatc gccaactggc gctgggcagg cgtaccgttc tacctgcgca
     1741 ccggcaagcg gctggccagc cgcgtttccg agatcgtcat cgagttcaag ccgatcccgc
     1801 attcgatctt cgagggcgag gcggcgggac cgatcttccc caaccagttg gtcatccggt
     1861 tgcaaccgga cgaaggcgtc aagcagttca tcatgatcaa ggatccgggt ccgggcggca
     1921 tgcggctgcg ccagataccg ctcgacatga gctttgcgca gtcgtttaag gggcgttcgc
     1981 cggacgccta tgagcggctc atcatggatg tcatccgcgg caaccagacg ctgttcatgc
     2041 gccgcgacga ggtcgaggcc gcgtggaaat ggatcgaccc gatccagaac gcctgggcga
     2101 atgcgcgtca ggaagttcaa ggctatacgg ccggcacctg gggtccatcg gcctccatcg
     2161 cgctgatcga gcgcgatggc cgcacctggc acgaaagcaa ctaatggcga ccagaaacta
     2221 ccaccggcac gatttttcga cgcgcgacga actggcggcg gctctggccg cctgcgtcgg
     2281 cggcatattg ggcgacgaga tcgccgccgc gcaggcggcc agcatcgcgg tgtccggcgg
     2341 ctcgacgccc gcccgcttct tcgaggtgct gtcgaatgtc gagctggact ggaaaagcgt
     2401 caacgtcacg ctgatcgatg aacgctacgt gcccgacacc gagccgcgct ccaatgcagg
     2461 gctggtgaag gccaacctgc tccaaaacag ggcggccggg gccaatttcc tgccgctcta
     2521 ccaaagcggc gatgcgggag ccgacatgaa ggaggcctcg cgcttcctgt ccaccttctt
     2581 caatccgccc gacgtcgcca ttctcggcat gggcaatgac ggccacaccg cgtccttctt
     2641 cccggatgcg gtggagctgg agaaactgct ggacccgcaa ggcacgcacg agatcgccag
     2701 cgtgcatgcg caaagtgccg gcgaactgcg gctgacgctg accttgccgc tgatcgcgcg
     2761 cgcgcgcttc atcgcgctcc acatcgaggg cgaggaaaaa caggcagcgc ttgaaaaggc
     2821 gctgtcgccc ggctccacgt tgccgatccg acgcatgatc gacgcggcga aaaccccagt
     2881 ggaaatcttc tgggcgcctt gagccggaca atcaaggacg gacgccatga ccgccagacg
     2941 cgacatcgaa gccatcaccg agcgcatccg cgagcgttcg cgcatcagcc gtgaaaccta
     3001 tctggggcgg atcgcggaag catcgggacg cgccgccaat cgcggtatgc tcgcctgcgg
     3061 caacctcgcc catggctttg ccgtatgcag cccttcggag aagatcgcgc tcagcggcga
     3121 caccgttccc aacctcggca tcgtcacctc ctataacgac atgctttcgg cgcaccagcc
     3181 gttcgaaacc tatccggcgc tgatcaagga ggcggcgaaa gaggcaggcg gcatcgcgca
     3241 ggtggcgggc ggcgtgccgg ccatgtgcga cggcgtcacc cagggccagc ccggcatgga
     3301 gctctcgctg ttttcgcgtg acgtgatcgc tatggcggcg gcgatcggcc tgtcgcacaa
     3361 catgttcgac gcggccgcct tcctcggcgt ctgcgacaag atcgtgcccg gattggcgat
     3421 cgcggcattg tcgttcggcc atttgccggc ggtgttcatt ccggccggtc cgatgacctc
     3481 cggcctgccc aacgacgaga aggccagggt gcgccagctt tatgcggagg gcaaggtggg
     3541 gcgcgccgaa ctgctggcgg cggaggcgaa gtcctatcac gggccgggca cctgcacgtt
     3601 ctacggcacc gccaattcga accagatgct gatggagatc atgggcctgc atatgccggg
     3661 cgcttccttc gtcaatccgg gcaccaggct gcgcgacgcg ctgacccgcg aagcgaccag
     3721 gcgcgcgctg gccatcaccg cgctgggcaa cgcctatacg ccggtcgggc gcatgatcga
     3781 cgagcgctcg gtcgtcaatg gcgtcgtcgg cctgcatgcc accggcggtt cgaccaacca
     3841 caccatccat ttgatcgcta ttgcggcggc agccggcatc aagctgacat ggcaggatat
     3901 ttccgacctg tcggaagcgg tgccgcttct ggcgcgtatc tatccgaacg gacttgccga
     3961 cgtgaaccat ttccaggcag ccggcggcat gggtttcctg atccgcgaac tgctcgatgc
     4021 gggcctgttg catgaggatg tgcagaccgt gtggggcgaa ggcctgcgcc cctacgcgat
     4081 agaggcgacg cttggcgcgg acggctccgt cgagcgcgcg cccgcgcccc ggcagagcgg
     4141 cgacgaaaag gtgctggccc cctacgccaa agccttccag ccgagcggcg ggctgaaggt
     4201 gctttccggc aatttcggcc acgccatcat caagacttcg gctgtcaagc cggagcggcg
     4261 gctgatcgaa gccccggcaa gggtgttcga cagccagcat gcgctgaacg acgccttcaa
     4321 ggctggcagc cttaccggcg atttcatcgc cgtcatccgc ttccaggggc cgaaggccaa
     4381 tggcatgccg gaactgcaca agctgaccac cgtgctcggt gtgctgcaag atcgcggcca
     4441 gaaggtcgcg ctggtgacgg acgggcgcat gtcgggcgcc agcggcaaag tgccggcggc
     4501 gatccatgtg acgccggaag cgctggaagg cgggccgatc ggcaaggtgc gggatggcga
     4561 tatcgtgcgt ctcgacgcag agatgggaac gctggaaatc ctggtgccgg caggcgatct
     4621 ggcgttgcgc ccggccgcca cggccgatct cagcgccaac gagttcggtt ttggccgcga
     4681 actgttcgcc ggcttccgcg ccttggtaac gcgcgccgac cagggcgcag ccgcgttcgg
     4741 aaacgcctga cgcacgcgcc ttttcccgcc gggtcggaaa ggcggcggaa ccgcctctcg
     4801 gctgccgacc ctattgaatc gtgacctcgg tgcgctcgcc ggcatgaacg gtggcggttt
     4861 tctcgctcgg cgcgccgccg cccgattttt cgacgacgat ggcataatcg ccggcgagga
     4921 cggcgggctg gaatttctcg tcataggcat agccgaccga cttgcgcttg ccatcggcgt
     4981 ccttcccgaa aatctcgatc cggttggcgc ccggcgcatc gatcgaaagc acgccggcat
     5041 cgaccgctac cttcagggcc cgttcctcgc cgaccttgac ggtgaacggc tgttcggcaa
     5101 ccgcaaggtc caccgtcgcc tggatgacgt aatcgccggc cgccagatcg aacttgctgt
     5161 cagaaccgta gccgtagccg acctgctcgc gcgtgccgtc gccggtcttt tcagccttga
     5221 acaccttgaa gccaacgccc gaactatcgg ccttttcgcc gcccgcgacg taataggcat
     5281 tggcgaccac atggccgacg ccgacgacga tgtccttttc cagcgtctgg ccggcggtta
     5341 gttggacggt atcatcgacc gaagcgctgc caagcgtcac cgtgatcttc tgctcaccgg
     5401 ccggcacgac gaggctggtc tcgccatagc gggtcgttgg aatgtcggcg cccggatagg
     5461 tgacggtgac cgccgcgcca cctgccatgt cgccgccggc gctggtgcgc gggcgcagcc
     5521 tgagcgtgcc ggcgttcagc accaggttcg gcctgtagac ctggccggcc tcgaccttga
     5581 gcggctgttc ggccttcgct tcgccgtcgc gggcgacaag gatgtaatcg cccggctcca
     5641 gattgccttg ataggctccg tattcggtgg cgatcgatgc gcctcgcgcg ccgttggcgg
     5701 cggccttgta tatttcccag gcattgcctt gcgtaatcgg cgcgccgtcc tgcgccaacg
     5761 tcacggtcgg gatgaaattg aattcaggcc tggccgtttc ggcggctgac ggcgctgccg
     5821 tttccggcgc ggcctgcgca gagggcgcag aggttgcctg cgccgcaggc gttgcagcga
     5881 cgctttcaac caaggccgcc tgaagcgcct tggcgtcgga tgcctcgata tatttgccgc
     5941 cggtgctctg cgcgaggcag gcgacctgtt ttccgtcctg cgccga
//
LOCUS       DASYCS010000221         8682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_632215,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000221 DASYCS010000000
VERSION     DASYCS010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 8682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8682
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632215"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VGM46_07080"
     CDS             complement(<1..204)
                     /locus_tag="VGM46_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018429697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192394.1"
                     /translation="MPGDAPRQRLPGPRHCLALDIAGRGLYFRSVFWILKHGTGTMTT
                     AQDRQRPQPRAGIMDIEAYVPGKS"
     regulatory      211..289
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            392..1330
                     /gene="metA"
                     /locus_tag="VGM46_07085"
     CDS             392..1330
                     /gene="metA"
                     /locus_tag="VGM46_07085"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009452412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HEY4192395.1"
                     /translation="MPIKIPDQLPAHDILAHEGVSVMDERTALRQDIRPLQIGLLNLM
                     PNKIRTETQFARLIGATPLQVELTLIRIGNHKAKNTSEEHLISFYRTWEEVRHRKFDG
                     FIVTGAPIELLPFEEVTYWRELKQIFDWTTTNVHSSFFICWGAMAAAWHFHQIPKYTL
                     ETKAFGVFRHRNNAPASPYLAGFSDDFAIPVSRWTEVRGSDFPARCGLDLLMESDKTG
                     PCLLAEPAGNRLYMFNHIEYDSTSLKEEYDRDIATGTPVAVPLGYYPDDDPARPPLNR
                     WRSHAHLLFGNWINQVYQTTPYNLEDVGPGPASAET"
     gene            complement(1401..3254)
                     /locus_tag="VGM46_07090"
     CDS             complement(1401..3254)
                     /locus_tag="VGM46_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEY4192396.1"
                     /translation="MANEESHAPADGKNAPKPASAVVRVSSLGDQIGTVRQALAGSPI
                     RGKLMFFGAAIIAIIVATSGGQILLNRWNAPFYDALQRRDLPDFLHQLVIFAEIAGAL
                     LVLNVGQTWLNQTIRLRLREALTLDLIDQWMRPRRAFLVAKAGAIGVNPDQRMQEDAG
                     HLADLTTGLGIGLLQSLILLASFVGVLWTLSSGFIFHFDGHSLAIPGYMVWAAIFYAG
                     SASWLSWLVGRPLIMQNSDHYAREADLRFSMVRANEHIDAIALGRGEPGEKRRLNLDL
                     AAVLDAIRRIYATQINLAWVTDTYGWITVIAPILVASPVYFAGDISFGGLMMAVGAFN
                     QVHSSLRWFINNIGGIADWRATLMRVADFRLALLETDVLHGTEQRIEMAQNEDARMTF
                     DGLEIVSPSGCTKLPEAHVSIGPGERVMVTGDASAGKTLFFRAIAGLWPWGSGRIGMP
                     AGAIPVFMPRTPYLASGTLRDVLTGGGQGDTFKVDEAKLEAVLEEVGLKRLAGSLGRP
                     ARWDQELNDDEQRLLAFAALALRKPDWVVIDEALDTFDGATTRRVLALLDKHLSKAAI
                     LNIGRGQHNLDFFPRSFAIAKDAEGKALQAPHIRTGAIEPPPQKAVAVKAK"
     gene            complement(3342..4124)
                     /locus_tag="VGM46_07095"
     CDS             complement(3342..4124)
                     /locus_tag="VGM46_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006200236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY4192397.1"
                     /translation="MHSDIVDLRAFYAGPLGRLTEHSISMALSTLWGTVPNERLVGLG
                     YTLPWLERFGSDAERVFAFMPATQGAVVWPATGPTATALVFDEELPLVDSSIDRMLLV
                     HSLEHAENPRETLNEIWRVLSPAGRVVIVVPNRRGVWARFEHTPFGIGRPFSRGQLTE
                     LLREANFTPAAWADALLFPPSRRGFMMRFHALLERMGRRLWPMFSGVIVVAAQKRIYQ
                     GVPIAQRASRRVFVPVLSPQGVAGMRRDGDGHGPRIGPPTHS"
     gene            4250..5017
                     /gene="gloB"
                     /locus_tag="VGM46_07100"
     CDS             4250..5017
                     /gene="gloB"
                     /locus_tag="VGM46_07100"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEY4192398.1"
                     /translation="MTIEIDQFMCREDNFGVLIHDPKSGATALIDAPEEKSILAAIER
                     TGWKPSLILTTHHHVDHVEANLALKERFKLRIIGPEAEKAKIPGIDETVGQGSVVPFG
                     DEKVEVIFTPGHTAGHVSYHFPQSKVLFAADTLFALGCGRLMECPPPVMFESLKKLAA
                     LPPETAVYCGHEYTLSNARFALTIDPTNTALQARAKKIEALRAEGKPTLPTTIGEELA
                     TNPFLRWADPAIRKNLGMENAADAEVFAEIRKRKDNF"
     gene            complement(5054..5803)
                     /locus_tag="VGM46_07105"
     CDS             complement(5054..5803)
                     /locus_tag="VGM46_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEY4192399.1"
                     /translation="MSGPASPAFAAGPRAFRGDYTVSFLGLSVARATFESRYENDAYS
                     IHGTVSSAGLAQLFDDTKGTITTSGRVLADRIEPAAFRADYTSGKKVSLIDIGFKDGK
                     VVSTKVVPPPKPRGADWLQLDMGDLAGIADPIAATVIRTDSPDKVCGRRVKLYDGEMR
                     ADLVLDQAEKGTISVKGYQGPTVTCRLGFEPVSGYRKGRKALEFLKKKSRIKVTFAPL
                     GQTGVYAPIYATVGTQIGTITVRARRFEATE"
     gene            6106..6402
                     /gene="rpmB"
                     /locus_tag="VGM46_07110"
     CDS             6106..6402
                     /gene="rpmB"
                     /locus_tag="VGM46_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEY4192400.1"
                     /translation="MSRTCELTAKSVMTGNNVSHANNKTRRRFLPNLVNVTLISEALN
                     QNVRLRVSANALRSVEHRGGLDAFLAKADVKELSQRARLLKKQIAKKLADQPAA"
     gene            6517..7197
                     /locus_tag="VGM46_07115"
     CDS             6517..7197
                     /locus_tag="VGM46_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HEY4192401.1"
                     /translation="MPFLSRYLPFVAAMALVVVASNVLVQFPMAGTVGGLSLADILTW
                     GAFTYPFSFLVTDLANRRYGPKVARRIVFVGFMTAIICSILIPPLLYRHGLIQFETAA
                     DRLVRIAAASGSAFLVAQLLDVTMFNRLRRQSWWRAPVAGTLAGSVFDTLVFFTVAFA
                     GAFAFAGPNDAFALETAPLMGVLPVETMRWVSWALGDLSVKLIIAVVALIPYRLIAQR
                     WSRPAVAA"
     gene            7194..7865
                     /locus_tag="VGM46_07120"
     CDS             7194..7865
                     /locus_tag="VGM46_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007702735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192402.1"
                     /translation="MIPWVQLDAAPLPEGGELRLKQRGHEFSIMLGANELMNSRLSGS
                     EEALASLSCERIRGRAAPNLLIGGLGMGFTLRAALTELGPDAKIAVAELVPAVVAWAR
                     GPMAAVFGACLDDPRVAIHETDVGLLIRAEAAQYDAILLDVDNGPEGLTRKGNDALYD
                     PRGLSQAHAALRPGGVLAVWSSAPDAGFTRRLTRTGFAVDEIKIRANGKRGARHTIWL
                     ATRKA"
     gene            complement(7920..8291)
                     /locus_tag="VGM46_07125"
     CDS             complement(7920..8291)
                     /locus_tag="VGM46_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007066847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY4192403.1"
                     /translation="MLKDKSSSAIVAVSNIEKARAFYRDILGLEIVDQSMGDVLVFRT
                     GATSLVVYRSDFARTNKANAVVWDVGDEIEPITENLKGKGVVFEHYDDIERKGDIHVA
                     GEMKMVWFKDPDGNILHLNNG"
BASE COUNT         1557 a         2889 c         2676 g         1560 t
ORIGIN      
        1 gctcttgccc ggcacatagg cttcgatgtc catgatgccc gcgcgcggtt gcgggcgttg
       61 ccgatcctgt gccgtggtca tcgtgccggt tccatgcttc aaaatccaga agacgctgcg
      121 gaaatacaag ccacgaccgg caatgtcgag tgccaagcaa tggcgcggcc ccggcaaccg
      181 ttgacgcggc gcatcgccgg gcatagatag aagccgttcc gtggtgattt ggccggtcgg
      241 cttgcagcca cgttaaagaa gtcgctaaaa aggccgagga tgacaagttc gttcagaccg
      301 gtcgcgattt tcgccgccgg tttttttgtt ttaagccggt cttttttgag cctgaccgtt
      361 ctggaggccg gtcttttttg gagcttgtca gatgccgatc aagattcccg atcagctgcc
      421 cgcccacgat attctcgccc acgagggcgt ctccgtgatg gatgagcgca ccgcgctgcg
      481 ccaggatatc cgtccgcttc agatcggcct gctgaacctg atgccaaaca agatccgcac
      541 ggaaacgcag ttcgcccgcc tcatcggcgc caccccgcta caggtggagc tgacgctgat
      601 ccgcatcggc aaccacaagg ccaagaacac ctccgaggag cacctgatct ccttctaccg
      661 gacctgggag gaagtgcggc accgcaagtt cgacggcttc atcgtcaccg gcgcgccgat
      721 cgagctcttg ccgttcgagg aagtcaccta ttggcgcgag ctgaagcaga tcttcgactg
      781 gacgacgacc aatgtccatt cctcgttctt catttgctgg ggggcgatgg ccgccgcctg
      841 gcatttccat caaatcccga aatacacgct cgaaaccaag gcgttcggtg ttttccgcca
      901 ccgcaacaat gcacccgcct cgccttatct tgccggcttc tccgacgatt tcgctattcc
      961 ggtttcgcgc tggacggagg tgcgcggcag cgactttccc gcccgttgcg ggctggacct
     1021 gctgatggag tccgacaaga ccggcccctg cctgctggcc gagcctgccg gcaaccgcct
     1081 ctatatgttc aaccacatcg aatatgattc gacgtcgctg aaggaagaat acgaccgcga
     1141 tatcgccacc ggaacgccgg tggcggtgcc gctcggctac tatcccgacg acgatccggc
     1201 gcgcccgccg ctgaaccgct ggcgctcgca cgcccatctg ctgttcggca actggatcaa
     1261 ccaggtctat cagaccacgc cgtataatct cgaagatgtc ggccccggcc ccgcgtcggc
     1321 cgagacctga aagacaacgg gccggggctg cttccccgcc cgctatttcg ctttcccggc
     1381 cggcctcttt tcccggccgg ctatttcgct ttcacggcta cggctttttg cggcggcggc
     1441 tcgatggcgc cggtgcggat atgcggcgcc tgcaacgcct tgccctcggc gtctttagcg
     1501 atggcgaagc tgcgcgggaa gaaatcgaga ttatgctgcc cgcgcccgat attcaggatc
     1561 gcggccttgg aaagatgctt gtccagcaga gccagtacgc gcctggtggt cgcgccatcg
     1621 aaggtgtcga gcgcctcgtc gatgaccacc caatcgggtt tgcggagcgc cagcgcggcg
     1681 aaggccagca gcctttgctc gtcgtcgttg agttcctggt cccagcgcgc cggccgtcca
     1741 agcgacccgg ccagccgctt cagcccgacc tcttccagca cggcctccag cttcgcctcg
     1801 tccaccttga aggtgtcgcc ttgtccgccg ccggtcagca cgtcgcgcaa cgtgccggag
     1861 gccagatagg gcgtgcgcgg catgaagacg gggatcgctc ccgccggcat gccgatgcgt
     1921 ccgctgcccc acggccacag gccagcaatg gcgcggaaga acagcgtctt gccggcgctg
     1981 gcatcgcccg tcaccatcac ccgctcgccc ggcccgattg aaacatgcgc ctccggcagc
     2041 ttggtgcagc cgctcggcga aacgatctcc agcccgtcga acgtcatgcg cgcatcctcg
     2101 ttctgggcca tctcgatgcg ctgctcggtg ccgtgcagca catccgtttc cagcagcgcc
     2161 agccgaaagt cggcgacgcg catcagcgtg gcgcgccagt cggcgatgcc gccgatattg
     2221 ttgatgaacc accgcaatga ggaatgaacc tggttgaacg cgcccaccgc catcatcagc
     2281 ccgccaaacg agatatcgcc cgcgaaatag accggcgagg cgacgaggat cggcgcgatc
     2341 acggtaatcc agccataggt gtcggtgacc caggcgaggt ttatctgcgt ggcgtagatg
     2401 cggcggatcg cgtcgagcac cgctgccagg tcgaggttca gccgccgctt ctcgcccggc
     2461 tcgccgcgcc ccagcgcaat cgcatcgata tgctcgttgg cccgcaccat cgagaagcgc
     2521 agatcggcct cgcgcgcata atggtcgctg ttttgcatga tcagcggacg cccgaccagc
     2581 cagctcagcc acgaggcgga gccggcatag aagatcgccg cccacaccat gtagccgggg
     2641 atcgccagcg agtgcccgtc gaaatggaaa atgaagcccg aagacagcgt ccacagcacg
     2701 ccgacgaagg aggcaagcag gataagcgac tgcaacaggc cgatgccgag gccggtggtc
     2761 aggtcggcca gatggccggc atcctcctgc atccgctggt ccggattgac gccgatggcg
     2821 cccgccttgg cgaccaggaa cgcccggcgc ggccgcatcc actggtcgat caggtcgagc
     2881 gtcaacgctt cgcgcaggcg caggcgaatg gtctggttga gccatgtctg gccgacattc
     2941 aagacgagaa gcgccccggc gatttccgcg aagatgacga gctggtgcag gaaatccggc
     3001 aggtcgcgcc gctgcaaggc gtcgtagaaa ggcgcgttcc agcggttcag caggatttgc
     3061 ccgccggagg tggcgacgat gatggcaatg atggccgccc cgaaaaacat aagcttgccc
     3121 cggatgggcg aaccggccag cgcctgccgc acggtcccga tctggtcgcc aaggctgctg
     3181 acccgcacga cggccgaagc cggcttcggc gcattcttgc cgtccgcggg agcgtggctt
     3241 tcttcattcg ccatggatgg cacctttata tctgccgacc cgatgccgga tcgcggccca
     3301 ccggttagca aaatagcgtt gaacagcgaa gtggcaggcg atcacgaatg cgtcggcggg
     3361 ccgatcctgg ggccatgacc atcgccatcg cggcgcatgc cggcaacgcc ttgcggcgac
     3421 agcaccggca cgaagacgcg gcgcgatgcc cgctgcgcaa tcggcacgcc ctggtagatg
     3481 cgcttttgcg cggcgacgac gatgacgccg gaaaacatcg gccacagccg ccgccccatg
     3541 cgctccagca atgcgtgaaa ccgcatcatg aagccgcgcc gcgagggcgg aaacagcagc
     3601 gcatccgccc aggcggcggg ggtgaaattg gcctcgcgca gcaattcggt cagttgcccg
     3661 cgcgaaaacg gccggccgat gccgaacggc gtgtgttcga agcgcgccca cacgccgcgc
     3721 cggttcggca cgacgatgac gacgcggccc gccggcgaca gcacccgcca gatctcgttc
     3781 agcgtttcgc gcgggttttc ggcatgttcc agcgaatgca ccagcagcat gcggtcgatc
     3841 gaggaatcga ccagcggcag ttcctcgtcg aacaccagcg cggtcgccgt cggcccggtc
     3901 gccggccaca ccaccgcccc ttgcgtcgcc ggcatgaagg cgaacacgcg ctcggcgtcg
     3961 ctgccgaacc gctccagcca cggcagcgta tagccgaggc cgaccagcct ttcgttcggc
     4021 accgttcccc acagcgtgga aagcgccatg ctgatcgaat gctcggtcag gcggccgagc
     4081 ggcccggcat agaaagcgcg aagatcgacg atgtcggaat gcatgggcct gcctgttgcg
     4141 agccatggac ggtagccgcg atgcaaggcg agttcaacaa agcatctcga aacgggtgac
     4201 agccggacct gcgggcacta tgtgtgcagc gaccatggga gaatttgaaa tgaccatcga
     4261 aatcgatcag ttcatgtgcc gcgaggacaa tttcggcgta ttgatccatg acccgaaaag
     4321 cggcgcgaca gcattgatcg atgcgcccga ggagaagtcc atccttgccg ccatcgagcg
     4381 caccggctgg aaaccctcgc tgatcctgac cacccaccac catgtcgacc atgtcgaagc
     4441 caatttggcg ctcaaggaac gcttcaagct gcgcattatc ggccccgaag cggaaaaggc
     4501 aaagattccg ggcatcgacg agacggtcgg acagggatcg gtagtgccgt tcggcgacga
     4561 aaaggtcgag gtgatcttca cgcccggcca caccgccggc cacgtctcct atcatttccc
     4621 gcagtcgaag gtgctgtttg ccgccgatac gctgtttgcg ctgggctgtg gccggctgat
     4681 ggaatgcccg ccgccggtga tgttcgaatc gctgaagaag ctcgccgcgc tgccgcccga
     4741 aacggcggtc tattgcggcc atgaatacac gctttccaac gcccgcttcg ccctgaccat
     4801 cgatcccacc aataccgcgc tgcaagcgcg ggcgaagaag atcgaggcat tgcgcgccga
     4861 gggcaagccg actttgccga ccacaatcgg cgaggagctg gcgaccaatc cgtttctgcg
     4921 ctgggccgac ccggcgatcc gcaagaacct cggcatggaa aatgccgccg acgcagaagt
     4981 gtttgccgaa atccgcaagc gcaaggataa tttctaggcc ggccgccacc cggcgcggcc
     5041 aaatttcgcc gttctattcc gttgcctcga aacgccgcgc gcgcaccgta atggtgccga
     5101 tctgcgtgcc gaccgtggcg tagatcggcg cgtagacgcc ggtctggcca agcggcgcga
     5161 acgtcacctt gatgcggctt ttctttttca ggaattcaag cgccttgcgg cccttgcggt
     5221 agccggacac cggctcgaag cccagccggc aggtcaccgt cggcccctgg tagcccttga
     5281 cggaaatcgt gcctttctcg gcttggtcaa gcaccagatc ggcgcgcatc tccccgtcat
     5341 agagcttgac cctgcgcccg cacaccttgt ccgggctgtc ggtgcggatg acggtggcgg
     5401 cgatcggatc ggcgatgccg gccaggtcgc ccatgtccag ttgcagccaa tccgcgccgc
     5461 gcggcttcgg cggcggcacg accttggtcg agacgacctt gccgtccttg aagcctatgt
     5521 cgatcagcga caccttcttg ccggacgtat agtcggcgcg aaaggccgcc ggctcgatcc
     5581 ggtcggccag cacgcggcca gatgtcgtaa tggtgccctt cgtatcgtcg aaaagctggg
     5641 caagcccggc gctggaaacc gtgccatgga ttgaataggc gtcgttctca tagcggcttt
     5701 caaacgtcgc cctggctacc gacaagccga gaaacgacac cgtataatcg ccgcgaaagg
     5761 cacgcggacc ggcggcaaag gccggcgagg cagggccgga catgccgacg cccgcaagca
     5821 cggtcgccag aagggcggca aaagagcgat gggcgtgagg catggccggc atggtccgag
     5881 cgggagcgcg tgagttgggg aaagcgtccc tctttgctgt ctggcccgtt gcttgccgcc
     5941 caattccggc gtttatatgg tggttcgccc ggaagcttgc gcttgacgtg caggcgaaat
     6001 ccccctatgg aacgccaact tttccataag gtcgcctgat cgagaccgcg tgcaaattgg
     6061 cgcggctacc aaggtttggg cgaacgagaa ctgaaggtag cacccatgtc ccgcacctgc
     6121 gaactcacgg ccaagtcggt catgaccggc aacaatgtga gccacgccaa caacaagacc
     6181 cggcgccggt tcctgccgaa cctggtcaac gtcacgctga tctcggaagc gctgaaccag
     6241 aacgtgcgcc tgcgcgtttc ggccaatgcg ctgcgctcgg tcgaacatcg cggcgggctc
     6301 gacgccttcc tcgccaaggc cgacgtcaag gagctgtcgc agcgcgcccg cctcctgaag
     6361 aagcagatcg ccaagaagct ggccgaccag ccggctgcct gaatttctcg gctgcctgaa
     6421 ttcgctgctt gaattcagac gggccaacca cttgattgaa gcgtgatccg gcatcgccgg
     6481 ccacgttccg ctggttccat tacccaggat aaaaaaatgc cttttctctc ccgttacctg
     6541 ccgttcgtgg ccgccatggc gcttgtcgtc gtggcgtcga acgtgcttgt ccagttcccg
     6601 atggccggca cggtcggcgg cctgtctctg gccgatatcc tgacctgggg cgcgttcacc
     6661 tatccgttct ccttcctcgt caccgatctc gccaaccgcc gctatgggcc gaaggtggcg
     6721 cgccgcatcg tcttcgtcgg cttcatgacg gccatcatct gctcgatcct catcccgccg
     6781 ctgctctacc gccatggcct gatccagttc gagacggcgg ccgaccgcct ggtgcgcatc
     6841 gccgccgctt ccggctcggc cttcctcgtc gcccagcttc tggacgtcac catgttcaac
     6901 cgcctgcgcc ggcaaagctg gtggcgcgcc ccggtggccg gcacgctggc cggctcggtg
     6961 ttcgacacgc tggtgttctt caccgtcgcc tttgccggcg ccttcgcctt tgccggccct
     7021 aacgatgcct tcgcgctgga gacagcgccg ctgatgggcg tgctgccggt cgagaccatg
     7081 cgctgggttt cgtgggcgct tggcgacctc tcggtcaagc tgatcatcgc ggtggtcgcg
     7141 ctcatcccct atcggctgat cgcccagcgc tggagccggc cggcggttgc cgcgtgatcc
     7201 cctgggtcca gcttgacgcc gcgccgctgc cggaaggcgg cgagcttcgc ctgaagcagc
     7261 gcggccatga attctcgatc atgctcggcg ccaacgagct gatgaacagc cgcctttccg
     7321 gctccgagga agcgctcgct tcgctgagct gcgagcgtat ccggggacgg gccgcgccaa
     7381 acctcctcat cggcgggctg ggcatgggct tcacgctgcg cgccgccctc accgaattgg
     7441 ggccggatgc gaagatcgcg gtggccgaac tggtgccggc cgtcgtcgcc tgggcgcgcg
     7501 ggccgatggc ggcggtgttc ggcgcttgcc tggacgatcc gcgcgtcgcc atccacgaaa
     7561 ccgatgtcgg cctactgatc cgcgccgagg ctgcgcaata tgacgccatc ctgctcgatg
     7621 tcgacaacgg ccccgaaggc ctgacccgca agggcaatga tgcgctttac gacccgcgcg
     7681 ggctttcgca ggcccatgcc gcgttacggc cgggcggcgt tctggcggtc tggtcgtctg
     7741 cgccggatgc cggcttcacg cgaaggctga cccgtacggg tttcgccgtc gacgagataa
     7801 aaatccgcgc caacggcaag cgcggcgcgc gccacaccat ctggctcgcc acccgcaaag
     7861 cctgacggcc tggcaatctt cgtcaggtct tgcaatcctc gtcagcccgg cagtcttcgt
     7921 tacccattgt tcaaatggag gatattaccg tccgggtcct tgaaccaaac catcttcatt
     7981 tcgccggcga catgaatgtc gcccttgcgt tcgatatcgt cataatgctc gaagacgacg
     8041 cccttgccct tgagattttc ggtgatcggc tcgatctcgt cgccgacgtc ccacaccacc
     8101 gcattggcct tgttggttcg cgcaaagtcg gagcgataga cgacaagcga ggtggcgccg
     8161 gtgcgaaaga ccagcacgtc gcccatgctt tggtcgacga tttcaagccc cagtatgtcc
     8221 cggtagaagg cgcgcgcctt ttcgatattg cttaccgcaa cgatagcgga tgaagatttg
     8281 tccttcagca tgatggttcc ttcccacgaa aagcccgccg ccacgaaccg gccttaccgc
     8341 tgtttacata acggatcggc cgtcgcttcg ttgcaagaac gccccaatgg gacgccccga
     8401 tgggtcgccc gcatgggtac ttgagtggga cgcccggatg gccggccatc cgacattttc
     8461 gtcggcgcag gcgtgggaac cgcctcgacc gcacgttttc tcgttgccac gcccggcatt
     8521 cgccgtctga ctgttgtcaa agaacgctgc ccttatgcag gggcaggtgg gaagcggggc
     8581 gcgaggccga gccttccaga gtgataagtt acgtggcgcg aacctcgcgt cccgccggtg
     8641 ttcttgcctc accgccggga ggcgcatttg cgcgcacaag cc
//
LOCUS       DASYCS010000222         3290 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_197345,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000222 DASYCS010000000
VERSION     DASYCS010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197345"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1189
                     /gene="serS"
                     /locus_tag="VGM46_07130"
     CDS             <1..1189
                     /gene="serS"
                     /locus_tag="VGM46_07130"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015317536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEY4192404.1"
                     /translation="LTELQARQERRNAASKEIGNAMRNGDSALAEKLKGEVGAIKDFI
                     QNGEARERELDKALNDALAVLPNVPLADVPVGKDEHDNVERRRFGDELIAQRRPRMGN
                     KPKEHFEIGEALGQMDFERAAKLSGSRFTVLKGQLARLERAIGQFMLDLHTTEHGYTE
                     VQVPLLVRDEALFGTGQLPKFEEDLFFAPHGDGRLGLIPTAEVPLTNLVREEITAHEK
                     LPLRMTALSPCFRSEAGSAGRDTRGMLRQHQFYKVELVSITDAESSIAEHERMTECAE
                     AVLKKLELPFRTMTLSTGDMGFGAQKTYDIEVWLPGQNAYREISSCSVCGDFQARRMD
                     ARYKVKDEKATRFVHTLNGSGTAVGRALIAVMENYQDEDGCVTIPEALKPYMGGLEKI
                     SAK"
     gene            1189..1947
                     /gene="surE"
                     /locus_tag="VGM46_07135"
     CDS             1189..1947
                     /gene="surE"
                     /locus_tag="VGM46_07135"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEY4192405.1"
                     /translation="MRILLTNDDGIHAEGLAVLERVARTLSDDVWVVAPETDQSGYAH
                     SLSISEPLRLRKIGEKHFAVRGTPTDCVIMGVKKILPAAPDLILSGINSGANVADDVT
                     YSGTVAGAMEGALLGIRSIALSQAYNYVGEERVVPYGTAETLAPALLKKLVAMPLPDG
                     VLLNVNFPNCPVEEVAGTVVAKQGKMVHSMWVDERRDGRGLPYYWLRFGRDAAEGKEG
                     TDLQALGERLVSVTPLHLDLTAHELRDQLARVLA"
     gene            2012..2665
                     /locus_tag="VGM46_07140"
     CDS             2012..2665
                     /locus_tag="VGM46_07140"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEY4192406.1"
                     /translation="MTLATDEREGFAAFLLRLRGRGTVPKALVAAFEATPRRGFISGH
                     FHSIAWSDRTMPIECGEAIEGADLQAAVIAALAIEPGNRVLEIGTGSGYTAAVMARLA
                     GRVVTVDRYKTLADQARQRFEALGIGNAMARQADGSNGLPNEGPFDRIVVWAAFDSLP
                     RFLLDQLSSGGIVIAPIGPEESEQVLAKLTKVGSRFEREDIGSVRLQPILPGIAAVL"
     gene            2762..>3290
                     /locus_tag="VGM46_07145"
     CDS             2762..>3290
                     /locus_tag="VGM46_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptigoglycan-binding protein LysM"
                     /protein_id="HEY4192407.1"
                     /translation="MQLSVLKANRRNLARGCAVLMVAGMAAGCSSQVARFGDGLDSVS
                     TASTSNQRSIIGQQEALQPFPGDTVAPAPVQASREAPVGRSSLPPVSSQSLPPVAQAE
                     APQRMPAAERPVRTAQLQAAPDIDRAVKTATVAKPFEAAQAEAPRLNDSQPRQKATEI
                     IVESGQTVSGLSHRYG"
BASE COUNT          593 a         1050 c         1065 g          582 t
ORIGIN      
        1 tctgaccgag cttcaggcca ggcaggaacg ccgcaacgcc gcctccaagg agatcggcaa
       61 cgccatgcgc aatggcgatt ccgcccttgc cgaaaagctg aaaggcgagg tcggcgcgat
      121 caaggatttc atccagaacg gcgaggcgcg cgaacgcgaa ctcgataagg cgctgaacga
      181 tgcgctggcc gtgctgccca acgtgccgct ggccgatgtc ccggtcggca aggacgagca
      241 cgacaatgtc gagcgccgcc gcttcggcga cgagttgatc gcccagcgcc ggccgcgcat
      301 gggcaacaag ccgaaagagc atttcgagat tggcgaagcg ctgggccaga tggatttcga
      361 gcgcgccgcc aaactctccg gctcgcgctt caccgtgctg aaaggccagc ttgcgcggct
      421 ggaacgcgcc atcggccagt tcatgcttga cctgcacact accgaacacg gctacaccga
      481 agtgcaggtg cccctgctgg tgcgtgatga ggcgcttttc ggcaccggcc agttgccgaa
      541 attcgaggag gatttgtttt tcgcgccgca tggcgacggc aggcttggtc tcatccccac
      601 cgccgaagtc ccgcttacga atctcgtgcg cgaggaaatc accgcccacg aaaaactgcc
      661 gctgcgcatg acggcgctta gcccgtgctt tcgttcggag gccggctcgg cgggcaggga
      721 tacgcgcggc atgttgcgcc agcatcagtt ctacaaggtt gagctggtgt cgatcaccga
      781 tgcggaaagc tcgattgccg agcatgagcg catgaccgaa tgcgccgagg cggtgttgaa
      841 aaagctcgaa ctgccgttcc ggaccatgac gctttccacc ggcgatatgg gttttggcgc
      901 gcagaaaact tacgacatcg aggtgtggct gcccggccag aacgcctacc gcgaaatctc
      961 gtcctgctcg gtctgcggcg atttccaggc gcgccgcatg gatgcccgct acaaggtcaa
     1021 ggacgagaag gcgacgcgct tcgtccacac gctgaacggt tcgggcacgg cggtcggccg
     1081 cgcgctgatc gcggtgatgg aaaattacca ggatgaggat ggctgcgtaa ccattcctga
     1141 agcgctgaag ccctatatgg gcggcctcga aaagatttcg gcgaaatgat gcgtattctg
     1201 ctcaccaatg acgatggcat ccatgccgaa ggcctggccg tcctcgaacg cgtcgcgcgc
     1261 acgctgtcgg acgatgtctg ggtggtcgcg cctgaaaccg accagtccgg ctacgcgcat
     1321 tcgctgtcga tttcggagcc gctgcgcctg cgcaagatcg gcgagaagca tttcgccgtg
     1381 cgcggcacgc cgaccgattg cgtcatcatg ggcgtcaaga aaatcctgcc cgccgcgccc
     1441 gacctgattt tgtcgggcat caattccggt gccaacgtcg ccgacgacgt cacctattcc
     1501 ggcacggtgg ccggcgccat ggaaggcgcg ctgctcggta tccgttcgat agcgctgagc
     1561 caggcctata attatgtcgg cgaggagcgc gtcgttccct acgggactgc cgaaacattg
     1621 gcgccggcgc tgctgaagaa gctggttgcc atgcccttgc cggacggcgt gctgctcaac
     1681 gtcaattttc cgaattgccc ggtcgaggaa gtggcgggca cggtcgtcgc caagcagggc
     1741 aagatggttc actccatgtg ggtggacgag cgccgcgacg ggcgtgggct gccctattac
     1801 tggctgcgct tcgggcgcga tgcggcggaa ggcaaggagg ggaccgatct tcaggcgctg
     1861 ggcgaacggc tggtttcggt gacgccgctg catctcgatt tgacggcgca cgagcttcgc
     1921 gatcaactgg ccagggttct tgcatgattc ctggcgccgg cccggcccca gttgccctga
     1981 ccacaggttt ttgcatgaca caggcgcgcg catgacgctg gctaccgatg agcgtgaagg
     2041 gtttgctgct ttcctgctgc ggctgcgggg caggggcacc gtgccgaaag cgctggtcgc
     2101 cgctttcgaa gccaccccgc gtcggggttt catctccggc catttccatt caatcgcctg
     2161 gtcggatcgc accatgccca tcgaatgcgg cgaggccatc gaaggggcag accttcaggc
     2221 cgccgttatc gccgcgctcg cgatcgagcc gggcaaccgc gtgctggaga tcggcaccgg
     2281 ctccggctac accgctgccg tgatggcgcg gctggccggc cgcgtcgtca ccgtcgaccg
     2341 ctacaagacg cttgccgacc aggcccgcca gcgcttcgag gcgctcggca tcggcaatgc
     2401 catggcgcgc caggccgatg gctcgaacgg acttcccaat gaaggcccgt tcgaccgcat
     2461 cgttgtctgg gcggcgttcg acagcctgcc gcgtttcctg ctcgaccagc tgtcgagcgg
     2521 cggcatcgtc atcgcaccga tcggcccgga agagagcgag caggtgctgg ccaagctgac
     2581 caaggtcggc agccgttttg agcgcgaaga catcggcagc gtgcggcttc agccgatcct
     2641 gcccggcatc gccgccgttc tttaataaaa ttcgcttcag aatcgagcag cttaacccgc
     2701 cggtaacatt aacgcgcttt aatcatgcat agttcgtacc gggtatgtgt gggttatcgc
     2761 gatgcagctc agtgttttga aggccaacag acgcaatctg gcgcgtggct gcgccgtcct
     2821 gatggttgcg ggcatggccg ccgggtgcag ttcccaggtg gccaggttcg gggacggtct
     2881 cgacagcgtc tccaccgcct cgacatccaa ccagcgctcg atcatcggcc agcaggaggc
     2941 tttgcagcct ttccccggtg ataccgttgc gccggcgccg gtccaggcca gccgcgaagc
     3001 gccggtcggc cgctccagcc tgccgccggt ttcatcgcag tcgctgccgc cggtagcgca
     3061 ggccgaggcg ccgcagcgca tgcctgccgc cgaaaggccg gtgcgcaccg cgcaattgca
     3121 ggctgccccc gacattgacc gtgcggtgaa aaccgcaacc gtcgcaaaac cgttcgaggc
     3181 cgcgcaagcc gaagccccgc gcctgaacga cagccagccg aggcagaagg cgacggaaat
     3241 catcgtcgaa agcgggcaga ccgtttccgg tctttcgcac cgctacggcg
//
LOCUS       DASYCS010000223         8746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3241153,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000223 DASYCS010000000
VERSION     DASYCS010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 8746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8746
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3241153"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="VGM46_07150"
     CDS             <1..354
                     /locus_tag="VGM46_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY4192408.1"
                     /translation="PFEQSAGYSRAVVQGDWCFVSGTTGYDYATMTMPETVEAQARNC
                     LATIAAALAQGGFELTDVVRARYYITDAAFVDRVFAILGEHFGEIRPAATMIVCGLNK
                     PEMKIEIEVTALRAA"
     gene            538..987
                     /gene="msrB"
                     /locus_tag="VGM46_07155"
     CDS             538..987
                     /gene="msrB"
                     /locus_tag="VGM46_07155"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEY4192409.1"
                     /translation="MPDYRKSEEAVSALSPEQYRVTQQSGTERPGTGEYLNNNEPGIY
                     VDIVSGEPLFASADKFDSHCGWPSFTKPIDPAYVNELRDASHGMVRTEVRSTHGDSHL
                     GHVFPDGPRDRGGLRYCINSASLRFIPRAEMQANGYGNYLDQVETVS"
     gene            984..1493
                     /gene="msrA"
                     /locus_tag="VGM46_07160"
     CDS             984..1493
                     /gene="msrA"
                     /locus_tag="VGM46_07160"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015916426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEY4192410.1"
                     /translation="MSNERAVLAGGCFWGMQDLIRRFPGVISTRVGYSGGDVPNATYR
                     NHGSHAEAIEIVFDPQKTSFRTLLEFFFQIHDPSTRDRQGNDRGASYRSAIFYTSDEQ
                     KLVAEDTIADVDASGLWPGKVVTELAPVSDFWEAEPEHQDYLERIPHGYTCHFVRPNW
                     KLPRRANAA"
     gene            1665..2771
                     /gene="leuB"
                     /locus_tag="VGM46_07165"
     CDS             1665..2771
                     /gene="leuB"
                     /locus_tag="VGM46_07165"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEY4192411.1"
                     /translation="MATKNLFLLPGDGIGPEAMGEVKKLIAAMNEKLGSGFSTDEGLV
                     GGSAYDAHGQAISDADMEKALAADAVLFGAVGGPKWDAVPYEVRPEAGLLRLRKDLEL
                     FANLRPAICYPALAASSSLKPEVVEGLDILIVRELTGGVYFGEPKQIIDLGNGQKRGI
                     DTQVYDTFEIERISGVAFELARTRNNHVTSMEKRNVMKSGVLWNEVVTQTHKARYADV
                     KLDHMLADSGGMQLVRWPKQFDVIVTDNLFGDMLSDIAAMLTGSLGMLPSASLGAPDA
                     RTGKRRALYEPVHGSAPDIAGQGIANPIAMIASFAMCLRYSFNMVAEADRLEAAIAAV
                     LDAGLRTKDIMSPGMTEVGTTRMGDAIIEKFLAA"
     gene            complement(2869..4719)
                     /locus_tag="VGM46_07170"
     CDS             complement(2869..4719)
                     /locus_tag="VGM46_07170"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY4192412.1"
                     /translation="MMFDTIVPALAQALEKRGYSELTPVQKAVVASELGQADALVSAQ
                     TGSGKTVAFGLALAPTLLGEAARFSQGGAPLALAVAPTRELALQVARELEWLYEMTGA
                     TIATCVGGMDMRTERRALERGAHIVVGTPGRLRDHITRHALDMTALKAVVLDEADEML
                     DLGFREDLEFILDAAPAERRTLMFSATVPRSIATLAKGYQRDAVRITAAGEEKQHHDI
                     EYRALAVAPADRENAIINVLRYYEAKNALVFCNTRAAVNHLTARFNNRNFSVVALSGE
                     LSQNERSHALQAMRDGRAKVCIATDVAARGIDLPNLELVIHADLPTNPETLLHRSGRT
                     GRAGRKGVSALIIPHHARRRTERLLQNAGITPEWASPPSADEVMRRDNERMLADSAFT
                     QPVADEEREFVTALMETHNAEQVAVAFLRLSRAGRSAPEDLAEPVAFRPSPERGERAV
                     ASYDDKPGRRDDFGDSVWFSLSVGRRQNAEPRWLIPMLCRSGGITKREIGAIKMQPEE
                     SFVQIAAASADRFFASLGPDHKVQGSITVKRLEGQPDLSRAGYNPPSPDKKPHRGKGS
                     FDNASPKRPFEKREKPAYAADAKPWASKSAKPKFDKSGPPKAKKAKKKPE"
     gene            5171..6205
                     /locus_tag="VGM46_07175"
     CDS             5171..6205
                     /locus_tag="VGM46_07175"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006110468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEY4192413.1"
                     /translation="MGFKVAVVGATGNVGREMLNILEERGFPISEIVPLASRRSLGSE
                     VSFGDKTLKTRVLDQYDFSDTDIVLMSAGGNVSKEWSPKIGKMGCVVIDNSSAFRTDP
                     DVPLIVPEVNPEAIDGFTRKNIIANPNCSTAQMLVALKPLHDAATIKRVVVATYQSVS
                     GAGKEGMDELFTQTRAVFVADTVDVKKFTKRIAFNVIPHIDAFMEDGTTKEEWKMVAE
                     TKKMLDPKIKVTATCVRVPVFIGHSEAVNIEFERPITADEAREILREAPGCLVIDKHE
                     DGGYATPIECAGEDATYISRIREDSTVDNGLNIWIVSDNLRKGAALNAVQIAELLIAR
                     GLISPKQKAA"
     gene            complement(6295..6699)
                     /locus_tag="VGM46_07180"
     CDS             complement(6295..6699)
                     /locus_tag="VGM46_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEY4192414.1"
                     /translation="MAGETDLKQLLRSMRPQLDANRYVFAILPLGQPMPDNLQPVMTF
                     HEDEGTTLVLRAEEAEAAGLAGAFACRRITLMVHSSLEAVGFLAAITARLAKAGMGVN
                     PVSAFFHDHLFVPAERAGEAMAMLEALARENG"
     gene            6896..7144
                     /locus_tag="VGM46_07185"
                     /pseudo
     CDS             6896..7144
                     /locus_tag="VGM46_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003527142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
     gene            complement(7332..7730)
                     /gene="rpsP"
                     /locus_tag="VGM46_07190"
     CDS             complement(7332..7730)
                     /gene="rpsP"
                     /locus_tag="VGM46_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010661492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEY4192415.1"
                     /translation="MSLKIRLARAGSKKRPYYHVVIADARSPRDGRFIEQIGSWNPLL
                     PKDGERVKVDADRVKHWLSHGAQPTDRVLRFLDEAGIVKRDTRSNPKKAEPGKKAQER
                     AALLKKAQEDAAAAVAAATAAPAEAEASAE"
     gene            complement(7780..8130)
                     /locus_tag="VGM46_07195"
     CDS             complement(7780..8130)
                     /locus_tag="VGM46_07195"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEY4192416.1"
                     /translation="MNDAEQARKILVDYRASIDNIDAALIHMLAERFRCTKAVGVLKA
                     THGLPPADPSREAEQIGRLRRLADEAHLDPDFAEKFLNFVVREVIRHHEQIAASSSGG
                     EKNAASSSGVTKTG"
     gene            complement(8127..8660)
                     /locus_tag="VGM46_07200"
     CDS             complement(8127..8660)
                     /locus_tag="VGM46_07200"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY4192417.1"
                     /translation="MTPRLETERLVLRAFAPGDVETFAPFYASDAASFVGGPENLVAT
                     WRRMAAYAGSWMLRGYGKFIVEEKEGGRPVGIVGPWFPEGWPEPEIGWTILPEFQGRG
                     YANEAAARSIAFAYSELGWTTAISAIFPGNERSFKLAERLGARHDGSVEIKPYGVLEI
                     YRHLPPSEFLGHSGRLP"
BASE COUNT         1570 a         2885 c         2687 g         1604 t
ORIGIN      
        1 cccttcgaac agagcgccgg ctattcacgc gccgtcgtcc agggcgactg gtgtttcgtc
       61 tccggcacca ccggctacga ctatgcgacc atgacgatgc ccgagaccgt cgaggcgcag
      121 gcccgcaact gcctggcgac catcgccgcg gcgctggcgc aaggcggctt cgaactgacg
      181 gacgtggtgc gagcgcgcta ctacatcacc gacgccgcct tcgtcgaccg cgtctttgcc
      241 attctcggcg aacatttcgg cgaaatccgc cctgcggcga caatgatcgt ctgcggcctc
      301 aacaagccgg aaatgaaaat cgagatcgag gtgaccgccc tcagggccgc ctgacgacag
      361 gacgcctcgc caaacgccat ccccgcccgg ctgctggccc ccagtctatc taccggccca
      421 ctctggctcc cgctattgcg caagctgtcg ccggatcaat cgattttgat cgctcttatt
      481 ggacttcggg cctgcgaata cccattttag gggaaaccaa acacatggga gtcaaacatg
      541 cctgactatc gcaagtcgga ggaggccgtt tccgcgctgt cgcccgaaca ataccgcgtc
      601 acccagcaga gcggcaccga gcggcccggt accggcgaat atctcaacaa caacgaaccg
      661 ggcatttatg tcgacatcgt ctccggcgaa ccgctgttcg cctcggcaga caagttcgat
      721 tcgcattgcg gctggccaag cttcaccaag ccgatcgatc ccgcttacgt caacgaactg
      781 cgtgacgcct cgcacggaat ggtccgcacc gaagtccgct cgacgcatgg cgacagccat
      841 ctcggccacg tctttcccga tggcccgcgc gatcggggcg gcctgcgcta ctgcatcaat
      901 tcggcctcgt tgcgcttcat tccccgcgcg gaaatgcaag ccaacggcta tggcaactac
      961 ctcgaccaag tggagactgt atcatgagta atgaacgtgc tgttcttgcc ggcggctgct
     1021 tctggggcat gcaggatttg atacgccgct ttcccggcgt gatttcgacc cgcgtcggct
     1081 atagcggcgg cgatgtcccc aacgccacct accgcaacca cggcagccat gccgaagcca
     1141 tcgagatcgt cttcgatccg caaaagacct cgttccgcac gctgctggaa ttcttcttcc
     1201 agatccacga tccgtcgacg cgggaccgcc agggcaacga tcgcggcgcc agctatcgct
     1261 cggccatctt ctataccagc gacgaacaga agctcgtcgc cgaagacacc atcgccgatg
     1321 tcgacgcatc cggcctgtgg ccgggcaagg tggtgacgga actggccccc gtcagcgatt
     1381 tctgggaagc cgagccggag caccaggatt atctggagcg gatcccacac ggctacacct
     1441 gccatttcgt gcgcccgaac tggaaactgc cgcgccgggc gaacgccgcc tgataaaaga
     1501 cgccacgccg caaggttggc aaaaagaccg ggcggccctc gtcgcccggt ctttttttat
     1561 ggccgggtag ctcacgttcc cggcaagtct gacgaagcat cttgcacgcc acccgcttgc
     1621 cgtttagcaa ggccgcgact gaaaacatct ttgggacggc tctcatggca acaaaaaatc
     1681 ttttcctcct gcccggcgac ggcatcggcc ccgaggcgat gggcgaagtg aagaagctca
     1741 tcgctgccat gaacgagaag ctgggcagcg gtttttcgac cgatgaagga ctggtcggcg
     1801 gctccgccta cgacgcgcat ggccaggcca tctcggatgc cgacatggaa aaggcgttgg
     1861 cggccgacgc cgtgctgttt ggcgcggtcg gcggcccgaa atgggatgcc gtgccttatg
     1921 aagtgcgccc ggaagccggc ctgctgcggc tgcgcaagga tctcgaactg ttcgccaatc
     1981 tgcgcccggc catctgctac cccgcgctcg ccgcttcttc ctcgctgaag ccggaagtgg
     2041 tcgagggcct cgacatcctc atcgtgcgcg aactgaccgg cggcgtctat ttcggcgaac
     2101 ccaagcagat catcgaccta ggcaacggcc agaagcgcgg catcgacacc caggtctacg
     2161 acactttcga gatcgaacgc atttcgggcg tcgcctttga gctggcccgc acccgcaaca
     2221 accacgtcac ctcgatggaa aagcgcaacg tcatgaagtc cggcgtgctg tggaacgagg
     2281 tggtcaccca gacgcacaag gccagatatg ccgacgtgaa gctcgaccac atgctggccg
     2341 attccggcgg catgcaattg gtgcgctggc ccaagcagtt cgacgtcatc gtcaccgaca
     2401 acctgtttgg cgacatgctc tcggatattg ccgccatgct gaccggttcg ctcggcatgc
     2461 tgccttcggc atcgctgggc gcgccggacg ccaggacagg caagcgccgc gcgctctacg
     2521 agccggtaca cggctccgcg ccggatatcg ccggccaggg catcgccaac cccatcgcca
     2581 tgatcgcctc cttcgccatg tgcctgcgct attccttcaa catggtggcc gaagccgacc
     2641 ggctggaagc cgccatcgcc gccgtgctcg acgcgggcct gcgcaccaag gacatcatgt
     2701 cgcccggcat gaccgaagta ggcaccactc ggatgggcga cgccatcatc gagaagtttt
     2761 tggcggcgta agttacccgc ccgctgtcga tacagccgtc accctcggca caaggccgag
     2821 gatgacggca agagttgcct ccgcactttc tccacagtac gaaaatgcct attccggctt
     2881 cttcttggct ttcttcgcct tcggcgggcc tgacttatcg aatttcggct ttgccgactt
     2941 gctcgcccac ggtttcgcat cggcggcgta ggcgggtttt tcgcgcttct cgaaggggcg
     3001 cttcgggctg gcattgtcaa agctgccctt gccgcgatgc ggcttcttgt cggggctggg
     3061 cggattatag ccggcgcgcg acagatcggg ctggccttcc agccgcttca ccgtgatgct
     3121 tccctgcacc ttgtggtcag gcccgaggct tgcgaaaaac cggtcggcgc tggcggccgc
     3181 gatctgcacg aagctttctt ccggctgcat cttgatcgcg ccgatctcgc gcttggtgat
     3241 gccgccgctg cggcacagca tggggatcag ccagcgcggc tcggcgttct gccggcgccc
     3301 gaccgacagc gaaaaccaga cgctgtcgcc gaaatcgtcg cgacggcctg gcttgtcgtc
     3361 gtaggatgca acggctcgct caccgcgctc cggcgaaggc ctgaacgcca ccggctcggc
     3421 aaggtcttcc ggcgccgaac gcccggcgcg gcttagtcgc agaaacgcca ccgcgacctg
     3481 ctccgcattg tgcgtttcca tcaacgcggt gacgaattcg cgctcctcgt ctgcgaccgg
     3541 ctgggtaaag gcgctgtcgg ccagcatccg ctcattgtcg cgccgcatga cctcatccgc
     3601 cgaaggcggg cttgcccatt cgggcgtaat gccggcgttt tgcagcaagc gctcggtgcg
     3661 tcgccgcgcg tgatgcggaa tgatcagcgc gctgacgccc ttgcgcccgg cacgtcccgt
     3721 acgcccgctg cgatgcagca gcgtttccgg gttggtcggc aaatcggcgt ggatcaccag
     3781 ttcgagattg ggcaggtcga tgccgcgcgc cgccacgtcg gtggcgatgc agaccttggc
     3841 gcggccgtcg cgcatcgcct gcaaggcatg gctgcgctcg ttctggctaa gctcgcccga
     3901 aagcgccacc accgagaaat tgcggttgtt gaaccgcgcg gtcaggtgat tgacggccgc
     3961 gcgcgtgttg cagaacacca gcgcgttctt ggcctcgtaa taacgcagca cgttgatgat
     4021 ggcgttttcg cggtccgccg gcgcaacggc aagcgcccgg tactcgatgt cgtggtgctg
     4081 tttttcctcg ccggcggcgg tgatgcgcac ggcgtcgcgc tggtagccct tggccagcgt
     4141 ggcgatcgaa cgcggcacgg tggccgaaaa catcagcgtg cggcgctcgg ccggcgctgc
     4201 gtccaggatg aattccaggt cttcgcgaaa accgaggtcg agcatctcgt cggcctcgtc
     4261 cagcaccacc gccttcagtg cggtcatgtc gagcgcatgg cgggtgatgt ggtcgcgcag
     4321 gcggccgggc gtgccgacga cgatatgcgc gccgcgctcc aacgcgcgcc gctcggtgcg
     4381 catgtccatg ccgccgacgc aggtggcgat ggtggcgccc gtcatctcgt agagccattc
     4441 cagttcgcgc gccacttgca gggcgagttc gcgggtgggc gcgacggcca gggccagcgg
     4501 cgcaccgccc tgcgaaaacc gcgccgcctc gcccagcagc gtcggcgcca gcgccaggcc
     4561 gaaggccacc gtcttgccgg aaccggtctg cgccgaaacc agcgcatcgg cctgccccag
     4621 ttcggaagcg acgaccgcct tttgaaccgg ggtcagctcg gaataaccgc gtttttccag
     4681 cgcctgggcc agcgcgggaa cgatagtatc aaacatcatg ttcatctttc ggaattctgg
     4741 gctttttggc acgccgggcg gctcatccgc ccaaggcacg tcgaggcaag cggtgcccgt
     4801 caaacgatct tggccgttcg tacttcgcgc ggccgccatt gtacaggtgc cgcgcgattg
     4861 actctttgcc caaaccgcgt aaaagccggc tccgaacagg gcatccttcc atgcgcggcc
     4921 ttctgatggc gtttttcgca ttcgacttcc ccggccacga tgcttccgca tcgggcgggc
     4981 gtgtcggctt gtccctgcat tcgaccaaaa ccacggccaa aaaaacgacc gccaaaacgg
     5041 tctgatctcc cttggcctgc tcgccctctc ccgggtctgg ccaaatgggg tcaggaattt
     5101 gggggacgaa cccgctccgg ccggcgggtt tctccaagcg aacaagcgga gagcgacagg
     5161 agaattttcg atgggtttca aggttgcggt agtcggcgcc acgggcaatg tgggccgcga
     5221 aatgctcaat attctggaag aacgcggctt cccgatcagc gagatcgtgc cgctcgcctc
     5281 gcgccgctcg ctcggctcgg aagtgtcctt cggcgacaag acgctgaaga cccgcgtgct
     5341 cgaccagtat gatttttccg ataccgacat cgtgctgatg tcggccggcg gcaatgtttc
     5401 caaggaatgg tcgcccaaga tcggcaagat gggctgcgtc gtcatcgaca attcgtcggc
     5461 cttccgcacc gatccggacg tgccgctgat cgtgccggaa gtgaaccccg aagccatcga
     5521 cggcttcacc aggaagaaca tcatcgccaa ccccaactgc tcgaccgcgc agatgctggt
     5581 ggcgctgaag ccgctacatg acgccgccac catcaagcgc gtcgtcgtcg ccacctacca
     5641 gtcggtctcc ggcgcgggca aggaaggcat ggatgaactg ttcactcaga cgcgcgccgt
     5701 gttcgtggcc gacacggtcg acgtgaagaa gttcaccaag cgcatcgcgt tcaacgtcat
     5761 cccgcacatc gacgccttca tggaagacgg caccaccaag gaagagtgga agatggtggc
     5821 cgaaaccaag aagatgctcg atcccaagat caaggtgacg gcgacctgcg tgcgcgtgcc
     5881 ggtgttcatc ggccactccg aggcggtcaa catcgaattc gaaaggccga tcaccgccga
     5941 cgaggcccgc gagatcctgc gcgaggcgcc cgggtgcctg gtcatcgaca agcacgagga
     6001 tggcggttac gccacgccca tcgaatgcgc cggcgaagac gccacctaca tctcccgcat
     6061 ccgcgaggat tcgaccgtcg acaacggcct caacatctgg atcgtctccg acaatctgcg
     6121 caagggggcg gcgctcaatg ccgtgcagat cgccgaactg ctgatcgccc gcggcctgat
     6181 ttcgccgaag cagaaagcag cctgaacaac cccactctat aggcgctgtc gcggcaacct
     6241 cgcgacagcg ccgacaggcg gagccaaaat ggcgtcgccg tcttgcgtga aagcctagcc
     6301 gttttcacgc gccagcgctt ccagcattgc catcgcctcg ccggcgcgct ccgccggcac
     6361 gaacaggtgg tcgtgaaaga aggccgacac cgggttgacg cccatgcccg ctttggcaag
     6421 ccttgccgtg atcgcggcaa ggaacccgac cgcttccaac gacgaatgaa ccatgagtgt
     6481 gatcctgcgg caggcaaacg cgccggccag ccccgccgcc tctgcctcct cggcgcgcaa
     6541 aaccagcgtc gtgccctcat cctcgtgaaa cgtcatcaca ggctggagat tatccggcat
     6601 cggctggccg agcggcaata tggcgaagac ataccggttc gcatcgagct gcgggcgcat
     6661 cgagcggagc aactgcttga gatcggtttc accggccatt gaaatcccgc tttatttcgc
     6721 cgcgcggctc gcctgcgcgc cggcctttgc gataaattac tgattcacca cgttaaacaa
     6781 tgtgcggcaa gccatcgccg gtagcctttc atttgccgtg ttcggatcga atcgtcgtat
     6841 cgtgcgtttt attgtgcttt gctgggctgg acattcattc tctgggagaa ccacgatgaa
     6901 gaagattttg gtgatgatgg cgctgctgct gccgattgca gcctgttcgc agacggagca
     6961 aggagcggcc attggcggcc tcggcggggc tgcgataggc agtgccgttg ccggtgatcc
     7021 gctggaaggc gccgttatcg gcggcgcggt cggcgcggtc ggcggcgcgc tgatcggcag
     7081 tgccaacgaa cgcggccagt gccgctaccg tgaccgctac gggcgggttt atgccggtcg
     7141 ctgcccaagc cgctatgacg accaccgcga tcgccgctac tacaagcgcc ggcactacaa
     7201 caattacggc ggctactgac accgttgcat tggcttcggc tgcacgggct gcggccggaa
     7261 gcctctggcc aaacaaaaaa gctgcgcgga tcggtcgatc cgcgcagctt tttcattcgt
     7321 ggaagatcgg cttactcggc cgaggcttcg gcttccgccg gggcggcggt cgcggcggca
     7381 acggctgccg cggcatcctc ctgcgccttc ttcaaaaggg ccgcgcgttc ctgcgccttc
     7441 ttgcccggct ctgccttctt cgggttggag cgggtgtcgc gcttgacgat accggcctca
     7501 tcgaggaagc gcagcacgcg gtccgtcggc tgcgcgccat gcgagagcca atgcttgacg
     7561 cggtcggcat cgaccttcac gcgctcgcca tccttgggca gcagcgggtt ccacgagccg
     7621 atctgctcga tgaaacggcc gtcgcgcggg ctgcgcgcat cggcgataac gacgtggtag
     7681 taaggacgct tcttggagcc cgcgcgggcc agtctgattt tcagtgacat tgttcttctc
     7741 ctaaaggctt ctattggtgg gttggattac ggctggtcgt cagccggttt tggttacgcc
     7801 gctgctcgaa gcggcattct tttccccgcc gctgctcgaa gcggcgattt gctcgtggtg
     7861 gcgaatgact tcgcgcacga cgaaattgag aaacttttca gcgaaatccg gatcgagatg
     7921 cgcctcgtcc gccaaccggc gcaggcggcc gatctgctcg gcttcgcggc tcggatcggc
     7981 gggcggcagc ccatgcgtcg ccttcagcac gccgaccgcc ttggtgcagc ggaaacgctc
     8041 ggccagcatg tggatcaggg ccgcatcgat attgtcgatg gatgcgcggt aatcgacgag
     8101 gattttgcgc gcctgttcgg catcgttcat ggcaacctcc cggaatggcc aaggaattcg
     8161 gatggcggaa gatggcgata gatttcaagc acgccatagg gcttgatttc caccgagccg
     8221 tcgtgccggg cgccaagccg ctcggcaagc ttgaacgagc gctcattgcc cggaaagatc
     8281 gccgatatgg ccgtcgtcca gccaagttcg ctgtaagcga acgcgatcga ccgcgccgcc
     8341 gcttcgttcg cgtaaccgcg gccctggaat tcgggaagga tcgtccagcc gatctcgggt
     8401 tcaggccagc cttccgggaa ccatggcccg acgatgccca ccgggcggcc gccctctttc
     8461 tcctcgacga tgaacttgcc gtagccgcgc agcatccacg atcccgcata agccgccatc
     8521 cgccgccagg tcgcgaccag gttctccggc ccgccgacaa agcttgcggc atcgctggca
     8581 tagaagggcg cgaaggtctc gacgtcgcca ggcgcgaagg cgcgcagcac gagacgttcg
     8641 gtttcgaggc gcggcgtcat ttcttgccgc cccttggcgg cagtccgcca cccagtccgg
     8701 gaagcttgat gccgccggga agacccggca atccgccagg caggcc
//
LOCUS       DASYCS010000224         1721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_341403,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000224 DASYCS010000000
VERSION     DASYCS010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341403"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            563..865
                     /locus_tag="VGM46_07205"
     CDS             563..865
                     /locus_tag="VGM46_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEY4192418.1"
                     /translation="MTGVSLFVELKAKAGKEEEVAAFLAGARGLAVAEAGTVTWYAVR
                     FDTDTFAIFDTFNDDAGRQAHLGGPIAAALMANAEELFAVPPEIRKADVLADKLPS"
     gene            complement(1056..>1721)
                     /gene="otsB"
                     /locus_tag="VGM46_07210"
     CDS             complement(1056..>1721)
                     /gene="otsB"
                     /locus_tag="VGM46_07210"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013530901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEY4192419.1"
                     /translation="HPDDVRVSGELRMLLETLETRLDGALAFITGRSITVVDHLFSPL
                     RLCAAGLYGLEHRLAVNGPVEAASEPQDIAALAQAIEAEFAGANVYIERKGPILALHT
                     RAAPHLLARAVELVEQALDTLPAGYRVLPGNAGVELMPLEAAKGAAIRRFMQLPLFAG
                     RKPVFLGDDTSDENGFEAVNEAAGLSIRVRPQGPTAAPHGLAGVTETIAWLEQCLQGD
                     AIR"
BASE COUNT          295 a          527 c          560 g          339 t
ORIGIN      
        1 cccgcttcag ctcccatcat gatgccgccg atactgttgg gcagggacca aaagccacct
       61 ccggtgatcg cgctgtagaa catccccgtg cctgctatcg cgacgccgcc aattatgccc
      121 gcgagagcgg aaaagaaaaa atccatgcaa atctccaagt tcaactgccg gtgatttatc
      181 gtgggcgcca gtggtctgtc gtggcgtccg cgatggctcg aagcgggtgg gtgcttatct
      241 tccaattcgc catagcgttg ggttatcgta ttcatggccg ttggagggac ggaggcgtta
      301 ctgccattgg ggtagaggga gtggctgccc gccttcgcgt cacgattcgc tggtgcgctc
      361 gttgattccc ggcctgccca tcggtcggga agggcgccgg cgtcgaagcc gggcgacttc
      421 acgggctgga tttgccgacg catactcgca ggatatcgtc gagacaggcg cgaccgcctt
      481 attttgccgc tttactctca tcgtaatgag atgcgattga ctcggtccga gcgtagacag
      541 cgcgcttcaa cggagaaaaa tcatgaccgg cgtttcactt tttgtcgaac tgaaagcaaa
      601 ggcggggaag gaagaggaag tagcggcgtt tctggctggc gcgcggggtc ttgccgtggc
      661 ggaagccggc accgtcacct ggtacgcggt ccgcttcgac accgacacgt ttgcgatctt
      721 cgatacgttc aacgacgatg cgggcaggca ggctcacctt ggcggaccga ttgccgccgc
      781 cctgatggcc aatgccgaag aactgttcgc ggtgccgccc gaaatccgca aggcggatgt
      841 tctcgccgac aagctgcctt cgtaacgcgg ttttccggcg gcccgctggt gccctgagtt
      901 tcgagatagc catctcgatt aggtgaagtc ggggccgtag ggcggcggga gcagggagtt
      961 ggtccggaaa acccgcgcgg gtgcatgctc gccgtgggtt ttcaaacccg ctcccttggc
     1021 caattccgct atatttcggg cggattgccg cgtctttatc ggatagcgtc gccttgcagg
     1081 cattgttcca gccacgcgat ggtttcggtc acgccggcaa ggccgtgcgg cgctgccgtc
     1141 ggtccttgcg gccggacgcg gatcgacagg ccggcggcct cgttgacggc ctcgaagccg
     1201 ttttcgtcgg aagtgtcgtc gcccaggaac accggtttcc tgccggcaaa gagcggcagc
     1261 tgcatgaaac ggcggatggc cgcgcctttg gccgcttcga gcggcatcag ttccaccccg
     1321 gcattgccgg gaagcacgcg gtagcccgct gggagcgtat ccagcgcctg ttcgacaagc
     1381 tcgaccgcgc gcgccagaag atgcggcgcc gcgcgcgtgt gcagcgccag gatcggtccc
     1441 ttgcgttcga tgtagacatt cgcgcccgcg aactcggctt cgatcgcttg cgccagtgcg
     1501 gctatgtcct gcggctcgct cgccgcctcg acagggccgt tcactgccag ccggtgctcc
     1561 agcccgtaaa ggccggcggc acacagcctg agcggcgaaa acaggtggtc gacgacagtt
     1621 atggagcggc cggtgatgaa ggccagcgcg ccatccagcc gcgtttccag cgtttccagc
     1681 agcattcgaa gctcaccgct gacccgcacg tcatccgggt g
//
LOCUS       DASYCS010000225         2077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3967983,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000225 DASYCS010000000
VERSION     DASYCS010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3967983"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="VGM46_07215"
     CDS             complement(<1..815)
                     /locus_tag="VGM46_07215"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY4192420.1"
                     /translation="MFEAEDHKEIREAVRKLCERFPGEYWRKLDAERAYPAAFVDALT
                     EAGYLATLIPEEYGGAGLTLSAAAAVLEEIQRSGGNGGACHAQMYVMGTVLRHGNEAQ
                     KQRYLPKVAAGELRLQAFGVTEPTSGTDTTSLRTFARREGDHYVINGQKIWTSRAEYS
                     DLMLLLARTTPRDQVAKRTDGLSVFILDMREALGNGLTIRPIRTMMNHATTEVFFDNV
                     RVPAENPVGEEGKGFRYILSGMNAERILIAAECVGDAKWLIEKASLYASQRNV"
     gene            complement(815..>2077)
                     /locus_tag="VGM46_07220"
     CDS             complement(815..>2077)
                     /locus_tag="VGM46_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY4192421.1"
                     /translation="LEQAALFVLDTLACALGALKTEPARILKAVAPLGAADTARRAFY
                     YGGLSHILEMDDLHRDSVTHPGCVVIPAALAVCARDNLGGVAFLEAVLAGYEACCRVG
                     MAAGKAHYRIWHNTSTCGPFGSAMAAAELIGLSEERTVWALGNAGTQSSGLWQFLETG
                     AMSKHLHTARAAESGVLAALMAAEGFTGPDEILEGKKGFFAGLCPDPQPEAVSAGPDR
                     PWELTRTSIKPWPCCRHTHPAIDAAIALHGEIGGAEIAHVAVGVYQATLDVCDRPLPQ
                     DPYSAKFSIQHCVAAALADGAVVQASFDADARARLAALRAMAEVGVAAGVDAAYPRAW
                     GAELTVDTVDGRRFTALRREAKGDPENPVTAAELSAKARAILAGGGIGDARAEALIAA
                     ILALPDDAPVRALGLFPTEKGGLDARNS"
BASE COUNT          316 a          688 c          729 g          344 t
ORIGIN      
        1 aacacgttgc gctggcttgc atagagcgag gctttctcga tcagccattt ggcgtcgccc
       61 acgcattcgg cggcgatcag gatgcgctcg gcattcatgc cggaaagaat gtagcggaag
      121 cccttgccct cttccccgac cgggttttcc gccggcacgc gcacattgtc gaagaacact
      181 tcggtggtgg cgtggttcat catggtgcgg atcgggcgga tggtcagccc gtttcccagc
      241 gcctcgcgca tgtcgaggat gaagaccgac agcccgtccg ttcgcttggc cacctggtcg
      301 cgcggcgtgg tgcgggcaag aagcagcatc aggtcggaat attccgcgcg gctcgtccat
      361 attttctggc cgttgatcac atagtggtcg ccctcgcggc gcgcgaaggt gcgcagcgag
      421 gtggtgtcgg tgccgctggt cggctcggtg acgccgaatg cctgaagccg caattcgccc
      481 gccgccacct tgggcaggta gcgctgcttc tgcgcctcgt tgccgtgccg cagcacggtg
      541 cccatgacgt acatctgcgc atggcaggcg ccgccattgc cgccggagcg ctggatttcc
      601 tccagcacgg cggcggccgc cgaaagcgtc agcccggccc cgccatattc ctcggggatc
      661 agcgtggcga ggtagccggc ctcggtcaac gcgtcgacga aggcggcggg ataggcgcgc
      721 tcggcgtcga gcttgcgcca gtattcgccg ggaaagcgct cgcacagctt gcgcaccgcc
      781 tcgcggattt ccttgtggtc ttcggcttcg aacatcagct attgcgggcg tcaagcccgc
      841 ccttttcagt gggaaacagg ccaagcgcgc gcaccggcgc atcgtccggc agcgccagta
      901 tggcggcgat cagcgcttcg gcgcgggcat ccccgatgcc gccaccggcc aggatcgcgc
      961 gcgccttggc agacagttcg gcagccgtca ccgggttttc agggtcgccc ttggcttccc
     1021 tgcgcaacgc cgtgaagcga cggccatcca cggtgtcgac ggtcaattcc gcgccccagg
     1081 cgcgtggata ggcggcgtct acgccggcgg cgacgccgac ctcggccatg gcgcgcaggg
     1141 cggccagccg cgcgcgggca tctgcgtcga agctcgcttg caccacagct ccatcggcaa
     1201 gggcggcggc cacgcaatgc tggatcgaga atttggcgct gtaggggtcc tgcggcagcg
     1261 gcctgtcgca aacgtcgagc gtggcctggt agaccccgac cgcgacatgc gcgatctcag
     1321 cgccgccgat ctcgccatgc agggcgatgg cggcgtcgat cgcggggtgg gtgtggcggc
     1381 agcagggcca tggcttgatc gaggtgcggg taagctccca cggccggtcc ggtccggcgc
     1441 tcaccgcctc gggttgggga tcggggcaaa ggccggcgaa gaagcctttc ttgccttcca
     1501 gtatttcatc cggcccggtg aagccttccg ccgccatcag cgcggcgagc acgccggatt
     1561 cggcggcgcg tgcggtgtgc aggtgcttgc tcatcgcgcc ggtttcgagg aactgccaca
     1621 ggccggaaga ttgcgtgccg gcattgccca gcgcccatac ggtccgctcc tccgacaggc
     1681 cgatcagttc cgccgcagcc atggccgagc cgaacggccc gcaggtcgag gtgttgtgcc
     1741 agatgcggta atgcgccttg ccggcggcca tgccgacgcg gcagcaggcc tcgtagccgg
     1801 ccagcaccgc ttccaggaaa gcgacgccgc cgaggttgtc gcgcgcgcaa acggcaaggg
     1861 cggcggggat gacgacgcag cccggatggg tgacggaatc gcggtgcagg tcgtccattt
     1921 cgagaatgtg cgaaagcccg ccataataga aggcgcggcg cgcggtgtcg gcggcgccaa
     1981 gcggcgcgac agccttgagg atgcgcgccg gctcggtctt cagcgcgccc agcgcgcagg
     2041 ccagcgtgtc gagcacgaac agcgccgcct gttcgag
//
LOCUS       DASYCS010000226         3933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_341879,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000226 DASYCS010000000
VERSION     DASYCS010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3933
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341879"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VGM46_07225"
     CDS             complement(<1..488)
                     /locus_tag="VGM46_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY4192422.1"
                     /translation="MLSHERVWAAIDALAERYSLSASGLAKRAGLDSTAFNKSKRLAS
                     DGRPRWPSTESLAKIIEATGASLDEFMALLNGGREDADALPVQRPTVPLMGFAQAGAG
                     GFFDDAGFPAGHGWDLIELPAQSTAGSYALKVHGDSMLPLYRNGDVLIVEPGAVTRKG
                     DRV"
     gene            complement(582..1292)
                     /locus_tag="VGM46_07230"
     CDS             complement(582..1292)
                     /locus_tag="VGM46_07230"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY4192423.1"
                     /translation="MAGIKGILFDKDGTLVDFQATWFAIGDRMAMEAAGEDRSEADRL
                     MQLAGYDFEARAFKSDSVFAAGTNADIVGLWYPGVDQDRKRAMIAKFDTVTAREGALK
                     AVALPGTKDAIASLHASGFRLGVATNDSTKGAEQTLLGLGIAQMFTAAYGYDAVANPK
                     PAPDTVLAFCDLTGLRPAEIAMVGDNRHDLEMARAGDCGLAIGVLSGTGTRQSLAAMA
                     DVLLDSIADLPAFLAARG"
     gene            complement(1298..2941)
                     /gene="pgi"
                     /locus_tag="VGM46_07235"
     CDS             complement(1298..2941)
                     /gene="pgi"
                     /locus_tag="VGM46_07235"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010913205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEY4192424.1"
                     /translation="MANSAFQKTLGALQDQHAAGLRDMRGAFANDSGRFSRFSAADGD
                     LLLDWSKCAVDARTMELLEELAKAADVEGRRAAMFSGKHINVTEDRAVLHTALRNLDG
                     KGLTVDGQDIQADVLSVLDAMGVFADAIRSGKAPGATGKKITDIVNIGIGGSDLGPAM
                     ATLALAPYHDGPRAHYVSNIDGAHIHDTLKGLSAETTLFIIASKTFTTVETMTNAATA
                     RKWVEKALGAAAVGKHFAAVSTALDLVAKFGIEKDRVFGFWDWVGGRYSVWGAIGLPV
                     MISVGPGNFRAFLGGAHEMDEHFRNTPLMRNLPALLGLVGWWHRVVCKYPARAVIPYD
                     QRLSRLPAYLQQLDMESNGKSVTLESDAAPTPTGPLVWGEPGTNGQHAFFQLLHQGTD
                     IIPIEFLAAAVGHEPQLKHHHDLLLANCLAQSEALMRGRTLEEARAQMLAKGMKPEQV
                     DKIAPHRVFSGNRPTVTILYRKLDPRTLGRLIALYEHRVFVEGTLYDINSFDQWGVEL
                     GKELATQLLPVVEGKENAAKKDASTAGLVGHIRHLRGLE"
     gene            3188..>3933
                     /locus_tag="VGM46_07240"
     CDS             3188..>3933
                     /locus_tag="VGM46_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008840650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEY4192425.1"
                     /translation="MPQRNDTAIWSGLFRISAESGQTLQAQIRQAIVAAILDRQIAAS
                     MPLPSCRILAEKLGVARGTVVLAFQQLVDQGFLVARERRGHFVNPEVLATPAKPHQKA
                     PDQANEIDWKARRQIAASDMPPPNKHDNWIKSSYPFVYGQFDPTLFPTAEWRECNRMA
                     LAVLEIRNWASDMVDRDDPLLIEQIQARLLPRRGIFANPDEIIVTLGAQHALYMLATL
                     LMAKGSKVAMEDPGYPDARSIFRLAGAEIQP"
BASE COUNT          721 a         1278 c         1252 g          682 t
ORIGIN      
        1 acgcggtcgc ccttgcgggt aaccgcgccc ggctccacga tcagcacatc gccattgcgg
       61 tagagcggca gcatggaatc gccgtggact ttcagggcat aggaacccgc cgtcgactgc
      121 gccggcagtt cgatcaggtc ccagccatgc cccgcgggaa agccggcgtc gtcgaaaaaa
      181 ccgccggcgc cggcttgtgc gaagcccatc agcggaactg tcgggcgctg caccggcaag
      241 gcatccgcat cttcccggcc gccgttcaac agcgccatga actcgtccag cgatgcgccg
      301 gtcgcctcga tgatcttggc cagcgattcc gtggacggcc agcgcggtcg gccatcggag
      361 gccagccgct tcgacttgtt gaaggcggtc gaatcaagcc cggcgcgctt cgccagacct
      421 gaagccgaca gcgaataccg ctcggcaagg gcgtcgatgg cggcccatac gcgctcatgc
      481 gaaagcatgg cgtgccctcc ttcgggacag gaaaaaatac ctcgactacg gtactagacc
      541 gacaatgcgc cgctgtcgag ccgaaacagc gccggcttcg cctaaccccg cgcggcgagg
      601 aaagccggca gatcggcgat cgaatcgagc aggacatcgg ccattgcggc cagcgactgg
      661 cgcgtaccgg tgccggaaag cacgccgatg gccagcccgc aatcgccggc gcgggccatt
      721 tcgaggtcgt ggcggttgtc gccgaccatg gcgatttcgg ccgggcgcag gccggtcagg
      781 tcgcaaaagg ccagcacggt gtcgggcgcc ggcttggggt tggcgaccgc gtcatagcca
      841 taggcggcgg tgaacatctg ggcgatgcca agccccaaca gcgtctgttc cgcccccttg
      901 gtcgaatcgt tggtggcgac gccgaggcga aagccggatg catgcaggct ggcgatggca
      961 tccttggtgc cgggcagcgc caccgccttg agcgcgccct ccctggcggt aacggtatcg
     1021 aatttggcga tcatcgcccg cttccggtcc tgatccaccc ctggatacca gaggccgacg
     1081 atatcggcgt tggttccggc cgcgaacacc gaatcgctct tgaaggcgcg ggcttcgaag
     1141 tcatagccgg caagctgcat cagcctgtcg gcctcgcttc gatcttctcc cgccgcctcc
     1201 atcgccatgc ggtcgccgat ggcgaaccat gtcgcctgga aatcgaccag tgttccgtcc
     1261 ttgtcgaaaa gaatgccctt gatgcccgcc aagcccgcta ctccaatccg cgcagatggc
     1321 ggatatgtcc tacgaggccg gcggtcgagg cgtccttctt cgcggcattt tccttgcctt
     1381 ccacgacagg cagcaattgc gtcgccagtt ccttgcccag ttccacgccc cactggtcga
     1441 aggaattgat gtcgtagagc gtgccctcca cgaagaccct gtgctcatag agcgcgataa
     1501 ggcggccaag cgtgcgcgga tcgagcttgc gatagaggat cgtcacggtc ggccggttgc
     1561 cggaaaagac ccggtgcggc gcgatcttgt cgacctgttc cggcttcatg cccttggcca
     1621 gcatctgcgc gcgcgcctcc tccagcgtgc ggccgcgcat cagcgcttcc gactgcgcca
     1681 ggcaattagc cagcaaaaga tcgtggtgat gcttcagctg cggctcatgg ccgacggccg
     1741 ccgccaggaa ctcgatggga atgatatcgg tgccctgatg cagaagctgg aagaaggcgt
     1801 gctggccgtt ggtgccgggc tcgccccaga ccagcgggcc ggtcggcgtc ggcgccgcgt
     1861 cgctctcaag cgtcacgctc ttgccgttcg attccatgtc gagttgttgc agataggccg
     1921 gcaggcgcga caggcgctgg tcgtagggga tgacggcgcg cgccggatat ttgcagacga
     1981 cgcggtgcca ccagccgacc aggccgagta gcgccggcag gttgcgcatc agcggcgtat
     2041 tgcggaaatg ctcgtccatc tcatgcgcgc cgccgaggaa ggcgcggaaa ttgcccggac
     2101 caaccgagat catcacgggc aggccgatgg cgccccacac cgaataacgg ccgccgaccc
     2161 aatcccagaa tccgaagacg cggtcctttt cgatgccgaa tttggcgacg aggtcgagcg
     2221 ccgtcgaaac ggcggcgaaa tgcttgccga ccgccgccgc tccaagcgcc ttctctaccc
     2281 atttgcgggc ggtggcggcg ttggtcatgg tctcgaccgt ggtgaaggtc ttcgaggcga
     2341 tgataaacag cgtcgtttcg gccgaaaggc ctttcagtgt gtcgtggata tgcgcgccgt
     2401 cgatgttgga gacgtagtgc gcgcgcggcc cgtcatggta gggggccagc gccagcgtcg
     2461 ccatggccgg gccgagatcg gaaccgccaa tgccgatatt gacgatgtcg gtgatcttct
     2521 tgccggtggc gcccggcgcc ttgccggatc ggatggcgtc ggcaaacaca cccatcgcat
     2581 ccagcaccga aagcacgtcg gcctgtatgt cctggccatc gacggtcagc cccttgccgt
     2641 ccagattacg cagcgcggtg tgcagcacgg cgcggtcttc ggtcacatta atgtgcttgc
     2701 cggaaaacat cgcggcccgc cggccctcga catcggcggc cttggccagc tcttccagca
     2761 attccatcgt gcgcgcatcg accgcgcatt tggaccagtc gagcagcagg tcgccatcgg
     2821 cggcggaaaa gcgcgagaaa cgcccggaat cattggcgaa tgccccgcgc atgtcgcgca
     2881 ggcccgctgc gtgctggtcc tgcaatgcgc caagtgtttt ttgaaaggcc gaatttgcca
     2941 attgtgcgac tccttatctt cgggttgccg ccaccttatc agacatggcg gcggcgcaag
     3001 agggcatcgc acaacggcca agcaaacttg ttcggcgtgg caagctggcg cagtggattg
     3061 ggccacgatc gatcactggc ataaatcgtt atgataagaa aaattcattg gcgcaacgcc
     3121 ttgtgccagg aatacggtcc actggtccac cgaagcgaaa aagggtggcc acgaaggagc
     3181 gcctgtcatg ccacagcgaa acgatacggc gatatggtcc ggcctcttcc gtatttcagc
     3241 tgaatccggc cagacattgc aggcgcagat caggcaggcg atcgtcgccg ccattctcga
     3301 ccggcagatc gcggcttcga tgccgctgcc ttcctgccgg atattggcgg aaaagctcgg
     3361 cgtggcgcgc ggcaccgtgg tgctggcctt ccagcaactg gtcgaccagg gttttctggt
     3421 ggcgcgcgag cggcgtggcc atttcgtcaa cccggaagta ctggcgaccc cggccaaacc
     3481 gcaccagaag gcccccgacc aggccaacga gatcgactgg aaggcgcgcc gccagatcgc
     3541 ggcgagcgac atgccgccgc ccaacaagca cgacaactgg atcaagtcgt cctacccctt
     3601 cgtctacggc cagttcgacc cgacgctgtt tccaaccgcc gaatggcgcg agtgcaaccg
     3661 catggcgcta gccgtgctgg aaatccgcaa ctgggcttcc gacatggtcg accgcgacga
     3721 tccgcttttg atcgagcaga tccaggcgcg gctgttgccg cggcgcggca tcttcgccaa
     3781 tcccgacgag atcatcgtga cgctgggcgc gcagcacgcg ctttacatgc tggcgacgct
     3841 tttgatggcc aagggttcga aagtggcgat ggaagacccc ggctaccccg acgcccgctc
     3901 gatcttccgg ctggccggcg cggaaatcca gcc
//
LOCUS       DASYCS010000227         5138 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3388749,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000227 DASYCS010000000
VERSION     DASYCS010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 5138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5138
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3388749"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VGM46_07245"
     CDS             <1..243
                     /locus_tag="VGM46_07245"
                     /inference="COORDINATES: protein motif:HMM:NF019448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HEY4192426.1"
                     /translation="AVVELGGGRRRPDERIDHSVGFSGLAPIGAQVARGDTLALVHAR
                     TREEAERAAMAVLGSYKTGPAKPSLKKPVVRRIAAR"
     gene            complement(256..819)
                     /locus_tag="VGM46_07250"
     CDS             complement(256..819)
                     /locus_tag="VGM46_07250"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HEY4192427.1"
                     /translation="MLRNLLILAIFAALSASVSISYQSNPQLFERLLSSATSRETGVA
                     KPAEIKLAARESNAATPQPAGRKVLVPSDGRGHYVAAFKLNGRQVDALIDTGATLVAI
                     NTSTARRIGISLSTSDFNQSVSTANGVTRAAIVNLDTLQIGKIAIDDVQAVVLDDKAL
                     QTNLIGMSFLNRLGKFQAQDSTLLLVQ"
     gene            complement(936..1565)
                     /gene="upp"
                     /locus_tag="VGM46_07255"
     CDS             complement(936..1565)
                     /gene="upp"
                     /locus_tag="VGM46_07255"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEY4192428.1"
                     /translation="MKGVTVVDHPLVQHKLSIMRKKETSTAGFRRLLREISLLLGYEV
                     TRNLELTTTTIETPMETMEAPILEGKKLVFASVLRAGNGLLEGLLDLVPAARVAHIGL
                     YRDHETLEAVEYFFKAPSDLADRLIIVVDPMLATANSAIAAIEKLKERGATNIRFLCL
                     LAAPEGIERFTSAHPDVPVFTASIDRQLNEKGYILPGLGDAGDRLYGTK"
     gene            complement(1664..2641)
                     /locus_tag="VGM46_07260"
     CDS             complement(1664..2641)
                     /locus_tag="VGM46_07260"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEY4192429.1"
                     /translation="MPLKAELHCHIEGAASPELVIRQARKYGGDTSPFIRDGAFVWHD
                     FTSFLAAYDFCASLFRSEEDYALLADHYLSSLARDGAIYSEIFTSPDHAIKAGLSPKT
                     YTDALGEGMVRARAKTGIESRMIVTGVRHIGVEAIESAARFAARCGHPLVAGFGVAGD
                     ERVGDVEDYVRAFEIAREAGLGITIHAGELLGWESVAAALDHIRPARIGHGVRAIENP
                     DLVRRIAAQGVVLECCPGSNIALGIYAGFADHPFPALKAAGCKVTLSSDDPPFFHTSL
                     KQEYDHAARHFGMDEKALAAITRTAIEAAFVDRKTKAALLARLNGASAR"
     gene            2744..3520
                     /gene="ubiE"
                     /locus_tag="VGM46_07265"
     CDS             2744..3520
                     /gene="ubiE"
                     /locus_tag="VGM46_07265"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEY4192430.1"
                     /translation="MSVERTRASGGMETSYGFRQVQAGEKQPLVNDVFHKVAKRYDIM
                     NDLMSAGMHRIWKDAMVAWLNPPKRQGWRVLDVAGGTGDVAFQIVEASHRHVHATVLD
                     INGSMLGVGRDRAEKKGLGANSDFVEANAEELPFEDGVFDAYTIAFGIRNVPRIDVAL
                     SEAFRVLKPGGRFMCLEFSEVDMPLLDKAYEAWSFNAIPKIGKAVTGDGEPYSYLVES
                     IRKFPNQAEFATMIGRAGFERVSFRNYSAGIAAAHSGWKL"
     gene            3534..5102
                     /gene="ubiB"
                     /locus_tag="VGM46_07270"
     CDS             3534..5102
                     /gene="ubiB"
                     /locus_tag="VGM46_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006339056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenylphenol 6-hydroxylase"
                     /protein_id="HEY4192431.1"
                     /translation="MSSLGAGLRLVRAGWVLVREGVVAALPGDQLSGLPKFGWRLARL
                     FTRRRALRYQRSDRLAKAVGRLGPSYVKLGQFMATRPDVVGNDIALDLAMLQDKMHTF
                     DKADAIRAIETSLGRPMHDLYASFGDAVAAASIAQVHSATVADKPEIVAVKVIRPGVR
                     RRFAHDLESFFLAARLQEKYIPSTRRLQPVAVAQTLAQTTKIEMDLRLEAAALSELGE
                     NTRDDPGFRVPSVDWERTGRDVLTMEWIDGIKMNDVAALAAAGHDLKGIAANLIQSFL
                     RHTLRDGFFHADMHPGNLFVEEDGTIVAVDLGIAGRLGKKERRFLAEILYGFITRDYL
                     RVAEVHFEAGYVPRQHNIAAFAQAIRAIGEPIHGQPAETISMAKLLTLLFEVTELFDM
                     QTRPELVLLQKTMVVVEGVARTLDPAFNMWKTAEPVVGDWIRGNLGPRGLLGDAREGV
                     GALVALARQAPEFAARTERLSREIDQMAEHGLRLDEATAKAIGKAEARATRSGRVALW
                     VIALALVYVAWKLA"
BASE COUNT          932 a         1524 c         1733 g          949 t
ORIGIN      
        1 gcggtggtgg agcttggcgg cggccgccgc aggccggacg agcggataga ccactcggtc
       61 ggcttcagcg ggcttgcccc catcggcgcg caggtggcgc ggggcgacac gctggcgctg
      121 gtccatgctc gcacgcgcga agaggctgaa agggcggcga tggcggtgct gggttcctac
      181 aagacaggcc cggcaaagcc ttcgctgaaa aagccggtcg tccggcggat tgcggcgcgt
      241 taggctaaac ttgcgctact gcaccaacag caaagtgctg tcttgcgcct ggaacttgcc
      301 cagcctgttc aggaagctca tgccgatcag gttggtttgc aacgccttgt cgtccagcac
      361 gacggcctga acgtcatcga tggcgatctt gccgatctgc aaggtgtcga ggtttacaat
      421 cgccgcgcgg gtgacgccgt tggcggtcga aaccgattga ttgaaatccg atgtgctgag
      481 cgaaatgccg atccgccgcg ccgtcgatgt gttgatcgcc accagcgtcg caccggtatc
      541 gatgagcgcg tcgacctggc gtccgttcag cttgaacgcc gccacatagt ggccccgccc
      601 atcggaaggc acgagcacct tgcggccagc cggctgcggc gttgccgcat tgctttcacg
      661 ggcggcaagc ttgatttcag ccggctttgc cacgccggtt tctctggaag ttgccgaaga
      721 cagcaggcgc tcgaacagtt gtgggttcga ctgatagctg atcgaaaccg aagcggagag
      781 ggcggcaaat atggcgagta tgagaagatt gcgcagcatg ggcggcccct tgaacagggc
      841 cagctaagac acgcgaatgg tgtttgcggc tttaatataa gcggttttgc agccgtttac
      901 cgcgcggttt cgtcaatgaa cggtaaatgc gttggctatt tggtgccgta caagcggtcg
      961 ccggcatcgc caaggccggg caggatatag cctttttcgt tcaactggcg gtcaatggag
     1021 gcggtgaaca ccggaacgtc cggatgcgcg ctggtgaagc gttcgatgcc ttcgggcgcc
     1081 gccagcaggc acaggaagcg gatgttggtc gcgccgcgct ccttcagttt ctcgatggcc
     1141 gcaatcgccg aattggcggt ggccagcatc gggtcgacga cgatgatgag ccggtcggca
     1201 aggtcgctcg gggccttgaa gaaatattcg acggcctcca gcgtttcatg gtcgcggtaa
     1261 aggccgatat gggcgacccg ggccgccggc acgaggtcga gcaagccctc aagcaggccg
     1321 ttgccggcgc gcagcaccga ggcgaacacc agtttcttgc cttcgagaat gggcgcttcc
     1381 atggtttcca tcggcgtttc gatggtcgtg gtggtcagtt ccaggttgcg cgtcacctca
     1441 tagccgagca gcagcgatat ttcacgcagt aaccgccgaa agccggccgt cgaagtctcc
     1501 ttcttgcgca tgatcgacag cttgtgctgc accagcggat gatcgacgac ggtgacgccc
     1561 ttcatggtct tctcctgaat ttccaattct tggatcgacc ctaacggcaa tgcacggccc
     1621 aaggaacggg gctggcgcgg cgattacggc ttttgcagaa gaatcagcgg gcggacgcgc
     1681 catttagccg ggcaagcaag gccgctttgg tcttgcggtc gacaaaggcg gcctcgatgg
     1741 cggtgcgggt gatggcggcg agcgcctttt cgtccatgcc gaaatgccgg gcggcatggt
     1801 catattcctg cttcagcgag gtgtggaaaa acggcgggtc gtcggaactc agcgtcacct
     1861 tgcagcccgc tgccttcagt gcggggaacg gatggtcggc aaagccggcg taaatgccaa
     1921 gcgcgatatt ggagccgggg cagcattcca gcacgacgcc ctgcgcggcg atgcggcgga
     1981 caaggtcggg gttttcgatg gcgcgcacgc cgtggccgat gcgcgcgggg cggatatggt
     2041 cgagtgcggc ggcgacactt tcccagccca gcaattcgcc ggcatgaatg gtgatgccaa
     2101 ggcccgcctc gcgagcgatt tcgaaggcgc gcacatagtc ttcgacatcg ccgacgcgtt
     2161 cgtcgccggc gacgccgaag ccggccacca gcgggtgtcc gcatctggcg gcgaagcggg
     2221 cggcgctttc gatcgcctcg acgccgatgt ggcgcacgcc ggtgacgatc atgcggcttt
     2281 cgatgccggt cttggccctg gcgcgcacca tgccttcgcc cagcgcatcg gtataggttt
     2341 tgggcgacag gccggccttg atggcgtggt ccggcgaggt gaatatttcc gaatagatgg
     2401 cgccgtcgcg ggcaaggctg ctgagatagt gatcggccag cagcgcatag tcctcttcgc
     2461 tgcggaaaag gctggcgcaa aaatcgtaag cggcaaggaa agaggtgaag tcgtgccaga
     2521 caaaagcgcc gtcgcggatg aaaggagaag tgtcgccgcc atatttacgg gcctggcgga
     2581 tgacaagctc gggcgatgcc gccccttcga tgtggcagtg aagttctgct ttcaaaggca
     2641 tccttgatcc ctgtcctaca gcggccttac accgcgcctt aaacaatagc gcccggtagc
     2701 ctgatcggct atggtcccgc cggttttaag gcggatcaca agaatgtcag ttgagagaac
     2761 cagagcttcg ggcggcatgg aaacgtccta cggcttcagg caggtgcagg cgggcgaaaa
     2821 gcagccgctg gtcaacgatg tgttccacaa ggtggccaag cgctacgaca tcatgaacga
     2881 tctgatgtcg gcgggcatgc accgcatctg gaaagacgcc atggtggcgt ggctcaaccc
     2941 gccgaaaagg cagggctggc gcgtgctgga cgtggctggc ggcaccggcg acgtggcctt
     3001 ccagatcgtc gaggccagcc accgtcatgt ccatgcgacg gtgctcgaca tcaatggttc
     3061 gatgcttggc gtcgggcgcg accgggccga aaagaaaggg cttggcgcga atagcgattt
     3121 cgtcgaagcc aatgccgagg aattgccctt cgaagacggc gttttcgacg cctatacgat
     3181 cgccttcggc atccgcaacg tgccgcgcat cgatgtggcg ctttccgagg cgtttcgcgt
     3241 gctgaagccc ggcgggcggt tcatgtgcct cgaattctcc gaagtcgaca tgccgctgct
     3301 cgacaaggcc tatgaggctt ggtcgttcaa cgcgatcccg aagatcggca aggcggtgac
     3361 cggcgacggc gaaccctatt cctatctggt cgaatcgatc cggaaattcc ccaatcaggc
     3421 agagttcgcg acgatgatcg ggcgggccgg gttcgagcgc gtcagcttcc gcaattattc
     3481 cgccggcatc gccgccgccc attcaggctg gaagctttga ggcaggtgcg gcaatgagca
     3541 gtctgggcgc cggcctgcgg ctcgtcagag ccggctgggt gctggtgcgc gagggcgtgg
     3601 tcgcggccct gcccggcgac cagctttccg gcctgcccaa attcggctgg cggctggcgc
     3661 ggcttttcac ccgccgccgc gcgctgcgct accagcgcag cgaccgcctg gccaaggcgg
     3721 tggggcggct cgggccttcc tatgtcaagc ttggccagtt catggcgacg cggccggatg
     3781 tggtcggcaa cgacatcgcg ctcgacctgg cgatgctgca agacaagatg catacctttg
     3841 acaaggccga tgcgatccgc gccatcgaga cttcgcttgg ccggccgatg cacgatctct
     3901 atgccagctt cggcgatgcg gtggccgccg cctcgatcgc gcaggtgcat tcggcaacgg
     3961 ttgcggacaa gcccgagata gtggccgtca aggtgatccg gccgggggtg aggcggcgct
     4021 tcgcccacga cctcgaaagc ttcttcctcg ccgcgcgctt gcaggaaaaa tacatcccgt
     4081 ccacgcggcg cctgcaaccg gtggcggttg cgcaaacgct ggcgcagacg accaaaatcg
     4141 agatggacct gcggctggag gcggcggcgc tgtcggaact gggcgagaac accagggacg
     4201 atcccggctt tcgcgtgccc tcggtcgatt gggagcgcac cggccgcgac gtgctgacga
     4261 tggaatggat cgacggcatc aagatgaacg atgtcgccgc actcgccgct gccggccacg
     4321 acctgaaagg catcgctgcc aatctcatcc agtcgttcct gcgccacacg ctgcgcgacg
     4381 gcttcttcca tgcagacatg catccgggca atctgtttgt cgaagaggac ggcaccatcg
     4441 ttgccgtcga tctcggcatc gccgggcggc tcggcaagaa ggagcgccgg ttcttggccg
     4501 aaattctcta cgggttcatc acgcgcgact atctgcgggt ggcggaagtg catttcgagg
     4561 ccggttacgt gccgcgccag cacaatatag ctgctttcgc gcaggctatt cgcgccatcg
     4621 gcgagccgat ccatggccag ccggcggaaa ccatctcgat ggccaagctg ttgacgctgc
     4681 tgtttgaagt gaccgaactg ttcgacatgc agacgcgacc cgaactggtg ctgctgcaaa
     4741 agaccatggt ggtggtggaa ggcgtggcgc gcacgctcga tccggccttc aacatgtgga
     4801 aaaccgccga accggtggtg ggcgactgga tacgcggcaa tctcggcccg cgcggcctgc
     4861 tcggcgatgc gcgcgaaggc gtcggtgcgc ttgtagcgct ggcgcgccag gcgccggagt
     4921 tcgctgcccg cacggaacgc ctttcccgcg agatcgacca gatggccgag cacggtttgc
     4981 gcctcgacga ggcgacggcc aaggccatcg gcaaggcgga agcccgcgcc acgcgctccg
     5041 gccgggtagc gctgtgggtg atcgcgctgg cgctggtcta tgtggcgtgg aagctggcgt
     5101 gatgttggca tgatgccagc ggtttgactg cattgata
//
LOCUS       DASYCS010000228         3828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1067909,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000228 DASYCS010000000
VERSION     DASYCS010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1067909"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /locus_tag="VGM46_07275"
     CDS             complement(<1..904)
                     /locus_tag="VGM46_07275"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEY4192432.1"
                     /translation="MGKPGVGKQWRICVLGGGAWGTALALAMLRAGHRVRLWARDEET
                     VAAINRRENRHYLPGITLDPGIEAMSDMAAALAGTDCVLAVTPAQSLRAVLAAAKDHM
                     PQSVPLVLCAKGIERETGALLSSIVGEILPDNPIAALSGPSFAADVAKGLPTAVVVAA
                     QNERMAGELAARFSARNLRCYSSADLVGVEIGGALKNVFAIAAGAVTGAGLGASAQAA
                     MVTRGFVELRRIGAAFGAKPETLMGLSGLGDLLLTCSTPQSRNFAYGLALGRGEELAG
                     RPLAEGVPTAGIAARIALERGIDAP"
     gene            complement(958..2061)
                     /gene="tsaD"
                     /locus_tag="VGM46_07280"
     CDS             complement(958..2061)
                     /gene="tsaD"
                     /locus_tag="VGM46_07280"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006201143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEY4192433.1"
                     /translation="MIRVLGIETSCDETAASVVALGDESTPAILSPKILSNVVFSQIE
                     EHAAFGGVVPEIAARAHVEALDGIIEAALADAGTSLDEIDAIAATAGPGLVGGLIVGL
                     MTAKAIAAAAGKPLIAINHLEGHALTARLTDAIAFPYLLLLVSGGHTQIVAVRGIGDY
                     QRWATTIDDALGEAFDKTAKMLGLPYPGGPNVEKAALSGNPARFAFPRPMKGSAQPDF
                     SFSGLKTAVRQAAGAIAPLTQQDVADICAAFQAAVADTLSDRVLRALTRFHETFPGVK
                     NPTLVVAGGVAANQAVKSALQGLCLAHGFTFVAPPLKLCSDNAAMIAWAGIERLRAGK
                     VEAGAFDFAPRSRWPLDSVSEPMIGSGRRGAKA"
     gene            2153..3079
                     /gene="hemC"
                     /locus_tag="VGM46_07285"
     CDS             2153..3079
                     /gene="hemC"
                     /locus_tag="VGM46_07285"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007008388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEY4192434.1"
                     /translation="MQTPLKIGTRGSALALAQARETRSRLMAAHGLPEDAFEIIAIST
                     SGDRIQNRALSEAGGKGLFTKELEEALFERRIDIAVHSSKDMPTQLPDGLELSAFLPR
                     EDARDAFIGRTAKSIVELPHGAKIGSSSLRRQALIRRLRPDLDVVLFRGNVQTRLRKL
                     DEGVAEGTILALAGLKRLGLAHIVTDLMSLEDFPPAPGQGAIGIEARIGDEAVARMLA
                     AIHHVPTGQALACERAFLGALDGSCRTPIGGYASIDGDRLSFSGLIISPEGTRSHTIE
                     AAGPAADAARIGAQAGADIRAKAGEAFFDGWQ"
     gene            3080..>3828
                     /locus_tag="VGM46_07290"
     CDS             3080..>3828
                     /locus_tag="VGM46_07290"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006327154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEY4192435.1"
                     /translation="MRRRVLVTRPEPGASRTANRLAALGLEPVGLPLTQMLALPVALA
                     GLPGGADRVAATSANALRLAPKALTAALAHLPCHAVGAKTADAARAAGFLCVHEGPGD
                     AGALADEIAALPQLREEAALPQLGEMAASPQLGEMAASPPGRKIVYLCGRVRLAAFEE
                     RLRTAGISVDPVETYDTLAIERESVEIVVRLSGAPVDAVLLYSAGAARAMLGVLGRLE
                     LAHLFANAMFLCLSDRVAASLGSVDGGKVRI"
BASE COUNT          607 a         1262 c         1343 g          616 t
ORIGIN      
        1 tcggcgcgtc gatgccgcgc tccagggcga tgcgggcggc gatgccggcg gtcggcacgc
       61 cttcggccag cggccgcccg gccagttcct cgccgcgtcc cagcgccagc ccataggcga
      121 aattgcgaga ctgcggggtc gaacaggtta aaagaaggtc gccaaggccc gacaggccca
      181 ttagggtctc cggcttcgcg ccgaaggccg cgccgatgcg gcgcagttcg acaaagccgc
      241 gcgtcaccat ggcggcctgc gcgctggcgc caaggccggc cccggtcaca gcgccggcgg
      301 cgatggcaaa gacgttcttc agcgcgccgc cgatctcgac gccgacaagg tcggcgctcg
      361 aatagcagcg caaattgcgg gcggaaaagc gcgccgccag ttcgcccgcc atccgttcgt
      421 tctgcgcggc caccaccacc gccgtcggca ggcccttggc gacgtcggcg gcaaagctcg
      481 gtccggaaag tgcggcaatc ggattgtctg gcaggatttc gccaacgatg ctcgacagca
      541 gcgcccccgt ttcgcgctcg atgcccttgg cgcacagcac cagcgggacg gattgcggca
      601 tgtggtcctt cgccgccgcc agcaccgcgc gcagcgactg cgccggcgtc accgccaaca
      661 cgcaatcggt cccggcaagc gcggcggcca tatcgctcat cgcctcgatg ccggggtcga
      721 gggtgatgcc gggcaggtaa tgccggtttt cgcgccggtt gatcgccgcg acggtttctt
      781 cgtcgcgcgc ccacagccgc acccggtggc cggcgcgcag catggccagc gccagcgcgg
      841 tgccccacgc gccgccgccg agcacgcaaa tccgccattg tttgccaacg ccgggtttac
      901 ccatgccggg cttgtcgtcg ctgggcttgc cctcgtcggg cttgtctgcg ccggcgttca
      961 tgcctttgcc ccgcgccggc cggagccgat catcggttcg gaaacgctgt ccagcggcca
     1021 gcgcgaacgc ggcgcgaaat cgaaagcgcc cgcctcgacc ttgccggcgc gcagccgctc
     1081 gataccggcc caggcgatca tcgcggcatt gtcggagcac agtttcagcg gcggcgcgac
     1141 gaaggtgaaa ccgtgcgcca ggcacaggcc ttgcagcgcg gatttgaccg cctgattggc
     1201 ggccacgcca ccggcgacca ccaaggtcgg gttttttacg ccgggaaatg tctcgtgaaa
     1261 gcgcgtcagt gcgcgcaaga cgcggtcgct gagcgtatcg gccaccgctg cctgaaacgc
     1321 ggcgcagata tcggccacgt cctgttgggt aagcggggca atggcgcctg cggcctgccg
     1381 cacggcggtc ttgaggccgg aaaaggaaaa gtcgggctgg gcggagccct tcatcgggcg
     1441 cggaaaggca aagcgggccg gattgccgga aagcgccgcc ttctcgacat tggggccgcc
     1501 cggatagggc aggcccagca tcttggccgt cttgtcgaaa gcctcgccca gtgcgtcatc
     1561 gatggtcgtc gcccatcgct ggtaatcacc gatgccgcgc acggcgacga tctgcgtatg
     1621 cccgccagaa accaaaagca gcaggtaggg gaaggcgatg gcgtcggtca gcctcgcggt
     1681 cagcgcgtgg ccttcgaggt ggttgatggc gatcagcggc ttgccggcgg cggcggcaat
     1741 cgccttggcc gtcatcagcc cgacaatcag cccgccgacg aggcccggac cggcggtggc
     1801 ggcgatggcg tcgatctcgt caaggctggt gcccgcatcg gcaagggctg cctcgataat
     1861 gccgtccagc gcctcgacat gggcgcgcgc ggcgatttcc ggcacgacgc cgccaaaggc
     1921 ggcatgttcc tcgatctggc tgaacacgac atttgagagg attttgggcg acaggatcgc
     1981 gggcgtggac tcgtcgccca gcgccacgac gctggccgcc gtctcgtcgc aactcgtctc
     2041 tatgcccaga acgcggatca attggtcgct ggtccccgaa gttgtgcggg cgtccgttcc
     2101 ccgctaggag tggaaaaccc ggcttgccgg gggttatcac ggacggcgcg ccatgcaaac
     2161 accattgaag atcggaacga gaggcagcgc gctggcgctg gcgcaggcgc gggaaacgcg
     2221 ctcgcggctg atggcggcgc atgggctgcc ggaagatgcc ttcgaaatca tcgccatttc
     2281 gaccagcggc gaccgcatcc agaaccgggc gctgtcggaa gccggcggca agggcctttt
     2341 caccaaggaa ctggaagaag cgcttttcga gcgccgcatc gacattgccg tgcattcgtc
     2401 caaggacatg ccgacgcaat tgccggatgg gctggaactg tcggccttcc tgccgcgcga
     2461 ggatgcccgc gacgccttca tcggcagaac cgcgaaatcg atcgtcgaac tgccgcatgg
     2521 cgccaagatc ggctcgtctt cgctgcgccg gcaggcgctt atccgccgct tgcggccaga
     2581 tctcgacgtg gtgctgtttc gcggcaatgt gcagacgcgg ctgcgcaagc tggatgaggg
     2641 cgtggcggag ggcaccattc tggcgctggc cggcctgaag cggctggggc tggcgcatat
     2701 cgtcaccgac ctgatgtcgc ttgaagattt tccgccggcg cctgggcaag gcgccatcgg
     2761 cattgaggcc aggatcggcg acgaggcagt cgcgcgcatg ctggcggcca tccaccatgt
     2821 gccgaccggc caggcgctgg cctgcgagcg cgccttcctc ggcgcgctcg acggttcatg
     2881 ccgcacgccc atcggcggct atgcgtcgat cgacggcgac aggctgtcgt tcagcgggct
     2941 gatcatctcg ccggagggca cgcgatcgca cacgatcgaa gccgccggcc cggcggcgga
     3001 tgcggcgcgc atcggcgcgc aggccggggc cgatatacgg gcgaaggccg gcgaagcctt
     3061 cttcgacgga tggcaatgaa tgcgccgccg cgttctggtg accaggccgg aaccgggcgc
     3121 atcgcgcact gcaaacaggc ttgccgcgct tggcttagag ccggtcgggt tgccgctgac
     3181 gcaaatgctg gcgctgccgg tggcgctggc cggcttgccc ggcggcgccg acagggtggc
     3241 ggcgaccagc gccaatgcgc tgcgcctcgc gcccaaggcg ctgacagcgg cgctggcgca
     3301 tctgccttgc catgccgtcg gcgcgaagac agcggatgcg gcgcgcgccg ccggcttcct
     3361 ttgcgtccat gaaggaccgg gcgatgcggg cgcgctggcc gacgagatag cggctttgcc
     3421 gcagttgcgt gaagaagcgg ctttgcccca gttgggcgaa atggcggctt cgccccagtt
     3481 gggcgaaatg gcggcttcgc cgccgggccg gaagatcgtc tatctgtgcg ggcgggtgcg
     3541 gcttgcggcc ttcgaggaaa ggcttcgcac ggcaggcata agcgtcgatc ccgtcgaaac
     3601 ctacgatacg ctggcgatag agcgcgaaag cgtggaaatc gtggtgcgcc tgtccggtgc
     3661 gccggtcgat gcggtgctgc tctattcagc cggggcggcg cgggcgatgc ttggtgtgct
     3721 tggccgcctt gaacttgccc atctatttgc caacgccatg ttcctttgct tatctgatcg
     3781 cgtcgcggcg tcgctcggat ctgtcgatgg ggggaaagtc cgtatcag
//
LOCUS       DASYCS010000229         4589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4980523,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000229 DASYCS010000000
VERSION     DASYCS010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4589
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4980523"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..585
                     /locus_tag="VGM46_07295"
     CDS             46..585
                     /locus_tag="VGM46_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY4192436.1"
                     /translation="MRSFKLLLPLMLLIAGCKQDMADQPRYNPLAGSTQFADGMSART
                     PVEGTVTRNADLTPTPNDIPYPITMALLQRGQQRFDIFCSPCHGRTGEGHGMVVQRGF
                     PAPPTYHQEALRRAPDRHFYDVISHGYGAMYSYGARVPPADRWAIVAYIRALQYSRYA
                     SVGALPADLRTKFDAEVAR"
     gene            582..1715
                     /locus_tag="VGM46_07300"
     CDS             582..1715
                     /locus_tag="VGM46_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192437.1"
                     /translation="MNRPAWPDGYVAPAAVVAVAGLMLCGVGLFIAPSTMLAGWLTIA
                     LFLVGLPLGAMTVLMVHGLTGGRWGDALRPPLRAMVATLPLALLLLLPVLARPDLVFP
                     WAGADPSMLSEVVRQKLPYLNEPFFIVRSIACASAWMALGWLILARTAPDSGGPAGTS
                     FYAAGLVVHGLAVTVFAIDWMLSIEPDFYSTIYAMLEAAAEVVGACALALTVLAATRT
                     IETMPGGEEDAALGEDVANMLFGFMLMWAYLAFMQWIIVWAGDLPDEIHWYIVRGRDG
                     WQYWLWLLIALQFAVPFAGFLTRSIKRSHGGLLVLGVCVLSGHFIDMAWRVHPPLASA
                     GAPVSWPDFAATAGAGGAWLAFFFWKLSQPGYVSFRGRRHVHG"
     gene            1708..2199
                     /locus_tag="VGM46_07305"
     CDS             1708..2199
                     /locus_tag="VGM46_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192438.1"
                     /translation="MAKPLHPETRDVSPRALVVFGIGLVVFLALSAAALKLIFNTTPY
                     WPLANVETNDSEVSPAPQLSPATDLAAFRKQEDQELGKLAWVDRKAGIARVPIADAMK
                     LIAVQGLPDWTRQSVSTNQDCALLDGQVPRAPQASNCAPAGQPNGVEHPKADVAPSYG
                     ARP"
     gene            2196..2966
                     /locus_tag="VGM46_07310"
     CDS             2196..2966
                     /locus_tag="VGM46_07310"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEY4192439.1"
                     /translation="MTRWSRVSGLLLLSVLLAPKAAGALERPSFDPQIGAQLDIGKAF
                     HDASGQTSRLSEILDGRPALVIFGYDKCPNLCGVTQQAVASDLAKTGLDPETYRALFI
                     SIDPSETSQDATLAKTEVASSAGQKGLMAWRFLTSADNAGAALAAEAGITFDRRDRVA
                     QYIHPIAVMAVTPQGRIAQVLPALSFEPRDLRLALVEASAGRLGSIADHVFLLCAGFD
                     ASKGQYTSIIAATVKTGGVATILALAAGIFMLARRRRT"
     gene            2963..3919
                     /gene="coxB"
                     /locus_tag="VGM46_07315"
     CDS             2963..3919
                     /gene="coxB"
                     /locus_tag="VGM46_07315"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEY4192440.1"
                     /translation="MSFGIPFTPPQASSIAGNVDTLFYILLAFTFSLGVFLASLVVFY
                     AIKYRAGSKADRTGARTRSLPLEVAWTVASLLIAFVIFGWGAVLFLWREHPPMDALEI
                     AGLGKQWMWEFRHPGGQREINSLHVPVGKTVVVSLASQDVIHSFFVPAFRVKQDAVPG
                     RTTHVWFIANEPGKYHLFCAQYCGTQHSEMGGWVTVMPPEAYAAWLGNQGQGDTLAVD
                     GEKLFRALGCSGCHDGSSKVRAPDLAGVFGRPVALSNRQTIMADERYLRDSILDPNKE
                     IAAGYEPVMPSFDGLIDESETQMLLAYLKSLSPPATNSEATQ"
     gene            3916..>4589
                     /locus_tag="VGM46_07320"
     CDS             3916..>4589
                     /locus_tag="VGM46_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEY4192441.1"
                     /translation="MTTEALSHSSYLGEGAGPAAWLLTTDHKRVAWLYLISLTAFFFL
                     GGAMAVLMRIELATPAGDLVSDDVYNRLFSLHGIIMVWFFLVPSIPATLGNFLLPLMI
                     GARDLAFPRLNLASWYVFILGSLFALASVLFGGVDTGWTFYTPLSTLYANGFVSMAAL
                     GVFIVGFSTIMTGINFIVTIHKLRAPGLTWFRLPIFVWTMYATALVMVLATPVLAASL
                     ILIVLER"
BASE COUNT          727 a         1456 c         1418 g          988 t
ORIGIN      
        1 acaattgacg cggcttggcg cggaaaccgt cgaggaggtg gaggcgtgag atcgttcaaa
       61 ctccttttgc ctctcatgct gctgattgca ggctgcaagc aggatatggc cgaccagccg
      121 cgttacaatc cgctggcagg cagcactcag tttgccgacg gcatgtcggc aagaacgccc
      181 gttgaaggca cggtgacgcg caatgccgat ctgacgccaa cgccaaacga cattccatat
      241 ccgatcacca tggcgctgct ccagcgtggt cagcagcgct tcgatatctt ctgttcgcct
      301 tgccacggcc gcaccggcga gggccacggc atggtggtcc agcggggctt tcccgcaccg
      361 ccaacctacc accaggaagc gctgcgccgc gcacccgaca ggcatttcta tgacgtaatc
      421 agccatggct acggcgcgat gtattcctat ggcgcgcgcg tgccgccggc cgaccgctgg
      481 gcaatcgtcg cctatatcag agcgctgcaa tattcacgct atgcgtcggt tggcgcgctg
      541 ccggccgacc ttcgcaccaa atttgacgcg gaggttgcgc gatgaaccgg cctgcctggc
      601 ccgatggcta tgttgctccg gccgcggtcg tggcggttgc cgggcttatg ctttgcggtg
      661 tcggtctttt catcgcgcca agcaccatgc tggccggttg gctgacaata gcgctgttcc
      721 tggtcggatt gccgctcggc gcgatgactg tcctgatggt gcacgggctg accggcgggc
      781 gctggggcga tgcgctgcga ccgcctttgc gggccatggt cgccaccttg ccgctggcat
      841 tgctgttgct gctgccggtc cttgcgcggc ccgatctggt ctttccatgg gcgggggcag
      901 acccgtccat gctgtcggaa gtcgtgcgcc aaaaactccc ctatcttaac gagccgttct
      961 tcatcgttcg ttccatcgcc tgcgcgtcgg cctggatggc gctcggctgg ctcatccttg
     1021 cccgcacggc gcctgacagc ggcgggccgg ccgggacgag tttttatgcg gccggcctcg
     1081 tcgtccacgg tctcgccgtg acggtatttg ccatcgactg gatgctgtcg atcgagccgg
     1141 atttttattc gacgatctac gcgatgctcg aagccgcagc cgaggtcgtc ggcgcgtgcg
     1201 ccctggcgct gaccgtgctg gcggcgactc gcaccatcga gacgatgccg ggtggcgagg
     1261 aagatgcggc gctcggcgaa gacgtggcca acatgctgtt cggctttatg ctgatgtggg
     1321 cttaccttgc cttcatgcaa tggatcatcg tgtgggccgg cgatctgccg gacgaaatcc
     1381 actggtacat cgtgcggggg cgtgatggct ggcaatattg gctctggctg ttgattgcgc
     1441 tgcaattcgc cgtgccgttc gccggcttcc tgacgcgctc gatcaagcga agccatggcg
     1501 gcctgctggt gcttggcgtg tgcgtccttt ccggccattt catcgatatg gcctggcgag
     1561 ttcatccgcc gctggccagt gccggcgcgc ctgtctcatg gccggatttt gcggcgacag
     1621 caggcgcggg aggcgcgtgg ctcgccttct tcttctggaa attatcgcag cctggctatg
     1681 tctcgttccg gggccggagg catgtccatg gctaagcctc tgcatcccga aacccgcgat
     1741 gtctcacctc gggcactggt cgtcttcggc atcggtctcg tcgtgttcct ggcgctgtcg
     1801 gcggctgcgc tgaaactgat tttcaacacg acgccttact ggccgctggc gaatgtcgaa
     1861 acgaacgaca gcgaagtaag tccggcaccg caactgtcgc ccgcgaccga ccttgcggcg
     1921 tttcgcaaac aggaagacca ggaactgggc aagcttgcct gggtcgaccg caaggccggg
     1981 atcgcgcgcg ttccgatcgc agacgcgatg aaactgatcg ccgttcaggg attgccggat
     2041 tggacacggc aatccgtttc aaccaatcaa gattgcgcct tgctcgacgg acaggttccg
     2101 cgcgcgcctc aagccagtaa ttgcgctccg gcagggcagc ccaacggggt cgagcacccg
     2161 aaagcagatg tcgcgccctc ctatggagcg cggccatgac acgatggtca cgggtgtcgg
     2221 gtctcttgct cttgtccgtg ctgctcgccc caaaagccgc cggcgcgctg gagcgtccgt
     2281 ctttcgatcc gcagatcggc gcgcaactgg atatcggcaa ggcttttcac gatgcttccg
     2341 gccagacgag caggctgtcc gaaattctcg acggccgacc ggcgctcgtc atcttcggtt
     2401 acgataaatg ccccaacctt tgcggcgtca cccagcaggc ggttgcttcc gatctggcca
     2461 agacggggct cgaccccgag acctaccggg cgctgttcat ctcgatagat ccttcggaga
     2521 cctctcagga cgcaaccctc gccaaaaccg aagtcgcttc gtccgcagga cagaagggac
     2581 tgatggcttg gcggttcctg actagtgccg acaatgccgg tgccgcgctc gccgccgagg
     2641 ctggcatcac ctttgatcgg cgcgatcgag ttgcccaata tatccacccg atcgccgtca
     2701 tggcagttac gccacagggg cgcatcgccc aggtgctgcc ggctttgagc tttgagccgc
     2761 gcgatctcag gcttgcgctg gtcgaggcgt ctgccgggcg cctcggctcg atcgccgacc
     2821 atgtcttcct gctctgcgca ggcttcgatg cttcgaaggg ccaatacact tccatcatag
     2881 ccgcgacggt caagaccgga ggggtggcaa ccatactggc gctcgccgct ggcatattca
     2941 tgctggcgcg tcggaggcgg acatgagttt cggcattccc ttcacgccgc cgcaagcctc
     3001 gtcgattgcc ggcaacgtcg atacgctgtt ctacatcctg cttgccttca cattttcctt
     3061 aggcgttttc ctggccagcc tcgtcgtctt ctatgccatt aaataccgtg cgggttccaa
     3121 ggccgaccgc accggcgcgc ggacgcgcag cctgccgctg gaagtggcct ggaccgtcgc
     3181 cagcctgctc atcgccttcg tcatcttcgg ctggggtgcg gtgctgttcc tgtggcgtga
     3241 gcatccgccg atggacgcgc tggaaattgc cggcctcggc aagcagtgga tgtgggaatt
     3301 ccgccatccc ggcggccagc gcgagatcaa ctcgctgcac gtaccggtcg gaaagacggt
     3361 cgtcgtgtcg ctggcgtcgc aggacgtcat ccactccttc ttcgtgcctg ccttcagggt
     3421 gaaacaggat gcggtgcccg gccgcaccac gcatgtctgg ttcatcgcca atgaacctgg
     3481 aaagtatcac ttgttctgcg cgcaatattg cggcacccag cattcggaga tgggcggctg
     3541 ggtaacggtg atgccgccgg aggcctatgc ggcgtggctt ggcaatcagg ggcagggcga
     3601 tacgcttgcc gtcgatggcg agaagctgtt tcgcgcgctt ggctgctccg gctgccatga
     3661 tggctccagc aaggtgcgcg cgccggatct agcgggggtg ttcgggcggc cggtggcctt
     3721 gagcaaccgt cagaccatta tggccgatga gcgctatctg cgcgattcca tccttgaccc
     3781 gaacaaggaa atcgccgccg gctacgaacc ggtgatgccg agcttcgacg gtcttatcga
     3841 cgaaagtgaa acgcaaatgt tgcttgccta tctcaaatct ctttcgccgc cagcgaccaa
     3901 cagcgaggca actcaatgac caccgaggcg ctgagccatt cgagctatct gggcgagggc
     3961 gccggtcccg ccgcgtggct cttgaccacc gaccacaagc gcgtcgcctg gctctacctg
     4021 atttcgctca ccgccttctt ctttctcggc ggcgccatgg ccgtgctcat gcgcatcgaa
     4081 cttgcgaccc cggcgggcga tctcgtgtcg gacgatgtct acaacaggct gttctccctg
     4141 cacggcatca tcatggtgtg gtttttcctg gtgccttcga tcccggcgac gctcggcaat
     4201 ttcttgctgc cattgatgat cggcgcgcgc gatcttgcat ttccgcgcct caatctggcg
     4261 agctggtatg tctttattct gggcagcctg tttgcgctgg cgtcggtgct tttcggaggg
     4321 gtcgacacgg gctggacctt ctacacgccg ctctcgacgc tctatgccaa tggcttcgtc
     4381 tcgatggccg cccttggcgt cttcatcgtc ggtttttcga cgatcatgac cggcatcaat
     4441 ttcatcgtca caattcacaa attgcgggcg cccggcctca cctggttccg gttgccgatc
     4501 tttgtgtgga ccatgtacgc caccgccctg gtgatggtgc tggcaacccc ggtgctcgcc
     4561 gcttcgctga tcctgatcgt gctggaacg
//
LOCUS       DASYCS010000230         9317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1505440,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000230 DASYCS010000000
VERSION     DASYCS010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 9317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9317
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1505440"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1054)
                     /locus_tag="VGM46_07325"
     CDS             complement(<1..1054)
                     /locus_tag="VGM46_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015918481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY4192442.1"
                     /translation="MANDGGPAKRTTIYDLAQLAGTSASAVSSVLNGKWKQRRISTQL
                     AERIERLAKEQGYALNMQASLLRREESHLIGMIVPKYDNRYFGSIVELFEDMARSRGL
                     FPIVTCTRRDPELELEAARAMISHQVGWLIATGATNPDRISEVCMAAGVRSLNLDLPG
                     SLAPSIISDNYSGARELTRRLLHNGRLKYGALTPLIFVGGRASDHNTAERLRGFHDAH
                     LELGIVLPAENVLTCGYAAEKADRALSELMTDTATPPRGMFVNSTISLEGVLSWLRRC
                     RLDRPYEPVLGCFDWDPLVALLDENIEMVRQDVPRMVQAAFNIIEHGPSAEAIIQVPT
                     KLVGGAGKAEPHDQALS"
     gene            1306..2352
                     /locus_tag="VGM46_07330"
     CDS             1306..2352
                     /locus_tag="VGM46_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016555717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEY4192443.1"
                     /translation="MNFRGLFASTALIAASMTGAALAQDAATVAFLMPDQASTRYEQH
                     DYPGFKAEMEKLCAKCTVIYQNANADVALQQQQFNSVITQGAKVIVLDPVDSAAAAGL
                     VTLAQSQGVKVIAYDRPIPDKTADYYVSFDNEGIGQAIAQSLVQHLKATGVAEGAGVL
                     EINGSPTDAAAGLIRDGIHKGLEGSGYKTLAEFDTPEWAPPKAQEWAAGQITRFGADI
                     KGVVAANDGTGGGAIAAFKAAGVNPVPPVTGNDATIAALQLIISGDQYNTISKPSEIV
                     AAAAANVVVKFLSGTAPEAKTTLYNTPSELFVPAVVTAENIKAEIFDKGIQKPEEICT
                     GEYADGCKKLGITQ"
     gene            2426..3244
                     /locus_tag="VGM46_07335"
     CDS             2426..3244
                     /locus_tag="VGM46_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018235494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY4192444.1"
                     /translation="MTDQSRSASAPGEKILSLRNVSKRFGAVSALTDIDLDIFAGEVV
                     ALVGDNGAGKSTLIKILAGVYQPTAGTIEFNGREVALDDPGAALGIGIATVFQDLALC
                     ENLDVVANLFLGHELSPLRLDEVQMEVRAWTLLKELAARIPSVREPVASLSGGQRQTV
                     AIARSLLLNPKIIMLDEPTAALGVAQTAEVLNLIERVRERGLGVVIISHNMEDVRAVA
                     DRIVVLRLGRNNGVFTPEASNQELVAAITGATENAVSRRQERKTAETPHPEQHP"
     gene            3241..4491
                     /locus_tag="VGM46_07340"
     CDS             3241..4491
                     /locus_tag="VGM46_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007798827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY4192445.1"
                     /translation="MSENDSSQMPALDRSDARVRNDDGLSGAVRAFIDRVRAGDLGML
                     PVVVGLVLISVVFSALNPIFLAPNNLVNLLFDCATVGIISLGIVCVLLLGEIDLSVGS
                     MSGFASAVVGVLWVNMGWPVAGAIAVAILAGVLVGAIYAFLFNRLGMPSFIASLSGLL
                     AILGMQLYILGPTGSINLPYASPLVSFGQTMIMPAWLSQSLALVPGVVLILTGLSTMR
                     RRRQANLTAQPMTNLVVKAVVLTALLEFAVYYLNQGRGVPWMFGFFVLLVVLLNYALT
                     RTKWGRSMFAVGGNREAARRSGINVRRIYTSAFLLCSTLSAIGGVLSASRLASSSQQA
                     GTGDINLNAIAAAVIGGTSLFGGRGSAYSALLGIIVIQAISNGLTLLNLSSSLRYMIT
                     GGVLAIAVIVDSLARRSRVSHGRA"
     gene            4512..5240
                     /locus_tag="VGM46_07345"
     CDS             4512..5240
                     /locus_tag="VGM46_07345"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014230117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY4192446.1"
                     /translation="MSEALKGKVAAITGAASGIGLECARHMLAAGAKVVLIDRAEERL
                     NALCAEMGPNAFPLVVDLLNGKQLSAIAPRIVELAGGLDIFHANAGAYIGGPVAEGDP
                     DAWDRMLNLNINAAFRSVHAVLPHMIAQKSGDIVFTSSVAGVVPVVWEPIYTASKFAV
                     QAFVHSTRRQVSQHGVRVGAVLPGPVVTALLDDWPKEKMEEALANGSLMQPKEVAECV
                     LFMLTRPRNVTVRDLVILPNSVDL"
     gene            5297..6907
                     /locus_tag="VGM46_07350"
     CDS             5297..6907
                     /locus_tag="VGM46_07350"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018900931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEY4192447.1"
                     /translation="MAIRGSYFIGVDVGTGSARAGIFNRDGDMLASARHDIGLWREHG
                     ILAEQSSSDIWRAVCAATREALALSGIRADEVAGLGFDATCSLVVLGEGGAPLAVGSS
                     EDPERNIIVWMDHRATEQAQRINATGHPVLNYVGGSISPEMETPKLLWLKENRPHIFD
                     AAWQFFDLTDFLTWKATDDLARSTCTVTCKWTYLAHEERWDPSYFDAVGLGVLADESF
                     SRIGQNMRTPGTPLGQGLTPQAAAELGLSPGTPVGAGLIDAHAGGIGTVGIGGDPTVN
                     LGYIFGTSSCTMATTAQPVFVPGVWGPYFSAMVPGVWLNEGGQSAAGAAIDQLISFHP
                     ASEQARRNAEAEHLSVGNYLANLASGSAKGADPIALAKGLHVVPEFLGNRAPLADPFA
                     RAVISGLGMERDMESLVALYVAGLAGIGYGLRQIIETQQQAGIDIENVVLSGGAGQHD
                     LVRHMLANACDKPIVVTKAEEPVLLGAAILGAVASRSFPTVASAMAQLSRIGSTIPPT
                     GGKVARIHQTRYQGFKQLQSLGRELQEI"
     gene            complement(7130..8311)
                     /gene="bktB"
                     /locus_tag="VGM46_07355"
     CDS             complement(7130..8311)
                     /gene="bktB"
                     /locus_tag="VGM46_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012321371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketothiolase BktB"
                     /protein_id="HEY4192448.1"
                     /translation="MTESTFILSAARAAIGSFGGSLSRQTPSGLGAKLAQEAIRRAGL
                     DPSSVEHVVFGNVIPTAPEDAYVARMAAIGAEIPKEAPAMTVNRLCGSGLQAIISAAQ
                     TVMLGDVDVALAGGVEIMSRSPHYVQSARFGHKMGDVTMLDGLTGVLTDPFGNGHMGM
                     TAENVAENYQVSRKDQDAFAAESQRRALAAIEAGHFKSQIVPVEVASGRSLVTFDTDE
                     YPKSGTTADSLAALKPAFKKEGSVTAGNASGINDGAAAVVIASEKYVRTTGVTPLGRI
                     VAYSHSGVEPGIMGIGPVPAVRNLLRRSRMTLADFDVIESNEAFAAQAIAVNRELGLD
                     TDKVNPDGGAIALGHPIGATGAILAVKALYHLKRTGGRYGLVTMCIGGGQGIAIALER
                     V"
     gene            complement(8387..>9317)
                     /locus_tag="VGM46_07360"
     CDS             complement(8387..>9317)
                     /locus_tag="VGM46_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY4192449.1"
                     /translation="AADLHPLLAGIAAATGVTLMGSSTIANMPRGRGRLDLKSVPYPV
                     DAAVAELAVFDTVVLVNCPAPTGFFLYPGRPSLLHRPDANVLVLSREDQDAAVALRFL
                     ADELAVQPATVPARSSDSPEPHGPIEPYGFAASVARRLPKDAIVVNEALTLGGGFSAV
                     MPHAEQCDWLTVTGGAIGGGIPLATGAAVGARAMGRGDRRVVTLQADGSAAYTIQGLW
                     TQARENLPVTTLLLNNRSYAILLGEYAKVGAEPGQTASDMMSLDRPAIEWCRIAEGFG
                     VPSVAVSTLEQLDAAFADALSRTGPMLIDVQFP"
BASE COUNT         1681 a         3080 c         2875 g         1681 t
ORIGIN      
        1 gcgatagcgc ctgatcgtga ggctccgcct taccggcgcc gccgaccagc ttcgtcggca
       61 cctgtatgat cgcctcggcg ctcggtccgt gttcgatgat gttgaaagcc gcctgcacca
      121 tgcggggaac atcctgccga accatttcga tgttctcgtc aagcagcgcc accagcgggt
      181 cccagtcgaa acatcccagt accggttcgt agggccggtc gaggcggcaa cggcgcagcc
      241 agctcagcac gccttcgagc gagatcgtcg aattgacgaa catcccgcgc ggcggcgtgg
      301 ccgtatccgt catcagctcc gacagcgccc gatcggcctt ttccgccgca tagccgcatg
      361 tcagcacgtt ctcggcggga agaactatgc cgagttccag atgggcgtcg tggaaacccc
      421 gcaaccgttc cgccgtgttg tggtcgctgg cgcgcccgcc gacgaagatc aacggcgtca
      481 acgcgccgta tttcagccgg ccattatgca ggagcctgcg cgtcagctcc cttgcgccgg
      541 aataattgtc cgaaatgatg gaaggggcga gcgagccggg caggtccagg ttgaggctgc
      601 gcacgcccgc cgccatgcag acctccgaaa tccggtcggg attggtggcg cccgtcgcga
      661 tcagccagcc gacctgatgc gagatcatcg cgcgcgccgc ttccagttca agctccggat
      721 cgcgccgcgt gcaggtgacg atgggaaaca ggccgcgcga gcgggccata tcctcgaaca
      781 gctcgacgat cgagccgaaa tagcggttgt cgtatttggg cacgatcatg ccgatgagat
      841 gcgattcttc ccggcgcagc aggctggcct gcatgttgag cgcgtagccc tgctccttgg
      901 ccagacgctc tatcctttcc gccagctggg tgctgatgcg gcgctgcttc catttgccgt
      961 tgagcaccga gctgaccgcg ctggccgacg ttccggcgag ttgcgccaga tcgtagatcg
     1021 tcgttcgctt cgcggggccg ccatcatttg ccaagggaat ctctcctgga caccatcttg
     1081 acatcattgt tataaggcgg caacattgct tcatcgattg agcaagcttg cacaaacgat
     1141 gaagcatggg ttcttgcatc agatcgggag gagccggtcc ggccaccgcg atggcacgaa
     1201 acggaccgat ccaatcggtc aatcggaact gacggatctt ttcggaatgc ccgacaggcg
     1261 cttccgaaac aaattgagca catggaattg tccagggagg aaagaatgaa ttttcgtgga
     1321 ttgtttgctt ctacggcgtt gatcgccgcg agcatgacgg gtgctgcctt ggcgcaggac
     1381 gcggcaacgg tggcgttcct gatgccggat caggcctcga cccgctacga gcagcacgac
     1441 tatcccggct tcaaggccga gatggagaag ctctgcgcca aatgcaccgt gatctaccag
     1501 aacgccaatg ccgatgtggc gttgcagcag cagcagttca attcggtgat cacccagggc
     1561 gcgaaggtca tcgtgctcga tccggtcgac tccgccgccg ccgccggact ggtgacgctg
     1621 gcccagtcgc agggcgtcaa ggtcatcgcc tatgaccgcc cgataccgga caagaccgcc
     1681 gactactacg tatcgttcga caatgaaggc atcggccagg ccatcgcaca atcgctggtg
     1741 cagcacctga aggcgacggg cgtggcggaa ggcgccggcg tcctggaaat caacggctcg
     1801 ccgaccgacg ccgccgccgg gctgatccgc gacggcatcc acaaaggcct cgaaggctcc
     1861 ggctacaaga cgctggccga attcgacacg cctgaatggg cgccgccgaa ggcgcaggaa
     1921 tgggcggccg gccagatcac ccgcttcggc gccgacatca agggcgtggt tgcggccaat
     1981 gacggcaccg gcggcggcgc gatcgccgcg ttcaaggccg ccggcgtcaa tcccgtgccg
     2041 ccggtgaccg gcaacgatgc gacgatcgcc gccttgcagc tcatcatctc aggcgaccag
     2101 tacaacacca tctccaagcc ctccgagatc gtcgcggcgg cggcggccaa tgtcgtcgtc
     2161 aagttcctgt cgggcaccgc ccctgaagcc aagacgacgc tctacaacac cccttcggaa
     2221 ctgttcgtcc cggcagtggt cacggccgag aacatcaagg ccgaaatctt cgacaagggc
     2281 atccagaagc ccgaggaaat ctgcacgggc gaatatgcgg acggctgcaa gaagctcggc
     2341 attacgcagt aacctttaaa tccacgcgag ccggccgggg tcgccccggc cgggctttcc
     2401 caatccgcga tcgggcgata cgacaatgac agatcagtcg agatccgctt ccgcgccggg
     2461 cgaaaagatc ctcagcctgc gcaatgtttc caaaaggttc ggcgcggtat ccgcccttac
     2521 cgacatcgac ctcgatattt ttgccggcga ggtcgtcgcc cttgtcggcg acaatggcgc
     2581 cggcaagtcg acgctcatca agatactggc gggcgtctac cagccgacgg ccggaaccat
     2641 cgagttcaac ggccgcgagg tcgcgcttga cgatccgggc gccgcactcg gcatcggcat
     2701 cgccaccgtc ttccaggacc tcgctctgtg cgagaacctc gacgtggtgg ccaatctctt
     2761 cctcggccac gagctgtcgc cgctgcgcct ggatgaggtg cagatggaag tgcgcgcctg
     2821 gacgcttctg aaggaactgg cggcgcgcat tccttcggta cgcgagccgg tcgcctcgct
     2881 gtctggcggc cagaggcaga cggtggccat cgcgcgctcg ctgctgctca atcccaagat
     2941 catcatgctg gatgaaccca ctgccgccct tggcgtcgcg caaaccgccg aggtgctcaa
     3001 cctgatcgag cgcgtgcgcg agcgcgggct gggcgtcgtc atcatcagcc acaatatgga
     3061 agacgtgcgc gcggtcgccg accgcatcgt cgtgttgaga cttggccgaa acaacggcgt
     3121 cttcacgccc gaggcttcca accaggaact ggtggccgcc atcaccgggg cgacggaaaa
     3181 cgcggtgtcg cgccgccagg agcgcaagac ggccgaaacg ccccatccgg agcagcaccc
     3241 atgagcgaga acgactcaag ccaaatgccg gcgctcgacc gcagcgatgc gcgcgtgcgc
     3301 aacgacgacg gcctgtccgg cgctgtccgc gccttcatcg accgcgttcg cgccggcgat
     3361 ctcggcatgc tgccggtggt ggtcgggctg gtgctgatct cggtggtctt ttcggccctg
     3421 aacccgattt tcctggcgcc caacaacctg gtcaacctgc tgttcgattg cgccacggtc
     3481 ggcataatct cgctcggcat cgtctgcgtg ctcctgcttg gcgaaatcga cctgtccgtc
     3541 ggctcgatga gcggctttgc ttccgccgtc gtcggcgtgc tgtgggtcaa tatgggctgg
     3601 ccggtggccg gggcgatcgc ggtcgccatc ctggccggtg tcctggtcgg cgcgatctac
     3661 gccttcctgt tcaaccggct gggaatgccg agctttattg cgtcgctgtc cggcctgctc
     3721 gccatcctcg gcatgcagct ttacattctc ggcccgaccg gctcgatcaa cctgccctat
     3781 gcctccccgc tggtatcctt cggccagacc atgatcatgc cggcctggct gtcgcaatcg
     3841 ctggcgctgg ttccgggcgt cgtgctcatc ctgaccggcc tgtccaccat gcgccgacgc
     3901 cggcaggcaa atctgacggc gcagccgatg accaacctcg tcgtcaaggc ggtggtgttg
     3961 accgcgctgc tggaattcgc cgtctattac ctcaaccagg gccggggcgt gccgtggatg
     4021 ttcggcttct tcgtgctgct ggtggtgctg ctcaactacg ccctgacgcg cacaaaatgg
     4081 gggcggtcga tgttcgccgt cggcggcaac cgcgaggcgg cgcgccgctc cggcatcaat
     4141 gtccggcgca tctacacctc cgccttcctg ctttgctcca ccctctcggc tatcggcggc
     4201 gtgctgtcgg cctcccggct ggcctcgtcc agccagcagg ccggcaccgg cgacatcaac
     4261 ctgaacgcga tcgccgcggc ggttatcggc ggcaccagcc tgttcggcgg ccgcggcagc
     4321 gcctattccg ccctgctcgg catcatcgtc atccaggcga tctcgaacgg cctgaccctg
     4381 ctgaacctgt cgtcgtcgct gcgctacatg ataacgggcg gcgtgctggc aatcgccgtc
     4441 atcgtcgatt ccctggcgcg caggtcgcgt gtcagccacg gtcgcgcata aaccgcttac
     4501 ggagtttgaa aatgtctgaa gccttgaagg gcaaggtcgc cgccataacg ggcgcagcgt
     4561 ccggcatcgg cctcgaatgc gcccgccaca tgctggccgc cggcgcgaag gtggtgctga
     4621 tcgaccgcgc ggaggaacgc ctgaacgcgc tctgcgcgga aatgggaccg aacgccttcc
     4681 cgctggtcgt cgacctcctg aacggcaagc agctttccgc catcgcaccg cgcatcgtcg
     4741 aactggccgg cgggctggac atctttcacg ccaatgccgg cgcctacata ggcggccccg
     4801 tcgccgaggg cgatcccgac gcctgggacc ggatgctcaa cctgaacatc aacgcggcct
     4861 tccgctccgt ccacgccgta ttgccgcaca tgatcgcgca gaaatcgggc gacatcgtct
     4921 tcaccagttc cgtcgccggc gtcgtgccgg tggtgtggga acccatctac accgcctcga
     4981 aattcgccgt gcaggcattc gtccattcga cacgccgtca agtctcacag catggcgtgc
     5041 gcgtcggcgc ggtgctgccg ggaccggtcg tcaccgcctt gctggacgac tggccgaaag
     5101 agaagatgga ggaggcgctg gcgaatggca gcctgatgca gccgaaggag gttgccgaat
     5161 gcgtgctgtt catgctgacg cgcccgcgca acgtcaccgt ccgcgacctc gtcattttgc
     5221 cgaacagcgt ggacctgtga acccccggcg cattcctgtc gcagctttcg caaaggatca
     5281 gcagcaaagg ccggccatgg caatcagagg atcatatttc atcggtgtcg atgtcggcac
     5341 cggcagtgcg cgcgccggca tcttcaaccg ggatggcgac atgctggcat cggccaggca
     5401 cgatatcggg ctttggcgcg agcatggcat cctggctgag caatcgagca gcgacatctg
     5461 gcgcgccgtc tgtgccgcga cgcgcgaggc tctggcgctg tccggcattc gcgccgacga
     5521 ggtggccggc cttggatttg acgcgacctg ctcgctggtg gtgcttggcg agggcggcgc
     5581 gccgctggcg gtcggttcgt cggaagaccc ggagcgcaac atcatcgtgt ggatggacca
     5641 ccgcgccacc gagcaggcgc agcggatcaa cgccaccggc caccctgtcc tgaattatgt
     5701 cggcggttcc atctcgcccg agatggagac gcccaagctg ctctggctca aggaaaaccg
     5761 cccgcacata ttcgacgccg cgtggcagtt cttcgacctc accgatttcc tgacctggaa
     5821 ggccacggac gacctcgccc ggtcgacctg cacggtgacc tgcaaatgga cctatctggc
     5881 gcatgaggag agatgggacc ccagctattt cgatgcggtg gggcttggcg ttctggccga
     5941 cgaatccttt tcccgcatcg ggcaaaatat gcgcacgccc ggcacgccgc tcgggcaggg
     6001 actgacgcct caggccgccg cagaactggg cctttcgccc ggcacaccgg tgggcgccgg
     6061 gctgatcgac gcccatgccg gcggcatcgg caccgtcggc atcggcggcg acccgacggt
     6121 caatctgggc tacatcttcg gcacgtcgtc ctgcaccatg gcgaccacgg cgcagccggt
     6181 tttcgtgccg ggcgtctggg gtccgtattt ttccgccatg gttcccggcg tctggctgaa
     6241 cgagggcggc cagtcggccg ccggcgcggc gatcgaccag ctcataagct tccaccccgc
     6301 ctccgaacag gcgcgccgca acgccgaggc ggagcatctg tccgttggaa actacctggc
     6361 gaacctggca tccggaagcg ccaaaggcgc cgatcccatc gcattggcca aggggctgca
     6421 tgtggtgccg gaattcctcg gcaaccgcgc tcccctcgcc gaccccttcg cgcgcgccgt
     6481 tatttcgggg ctgggcatgg agcgcgacat ggaaagcctg gtcgcgctct atgtggcggg
     6541 tctggccggc atcggctatg gcctgcggca gatcatcgag acgcagcagc aggccggcat
     6601 cgacatcgag aacgtcgtgc tgagcggcgg cgcgggccag cacgaccttg tccgccacat
     6661 gctcgcaaac gcctgcgaca agccgattgt cgtgacgaag gccgaagaac ccgtgctgct
     6721 cggcgcggcc atcctcggcg cggtggccag ccggtctttc ccgacggtcg cttccgccat
     6781 ggcgcaactg tcacgcatcg gcagcaccat cccgcccacc ggcggcaagg tagcccgcat
     6841 ccaccaaacc cgctaccaag gcttcaagca actccagtct ctcggacgag agctacagga
     6901 gatatagagg gatcggctac gccagaccgc ccatgtggcc agacctcagc atatgtcacg
     6961 atgccggcgc gcagagcagg tggcgcaagg tctgccagct cgatctcaaa tagctgcttc
     7021 ttccgtggaa gacggagatt ggccatgtga gcgcttcact tccacaattg ccggttcacc
     7081 aggcagcggc gggatcgcga atgcatgcaa tgaacgccta ccttcgttat cagactcgct
     7141 caagcgcaat ggcgatgcct tgtcccccgc caatgcacat ggtgacaagg ccatagcgtc
     7201 caccagttct cttcaaatgg tacaacgcct ttacggccag aatggcgccg gtcgcaccaa
     7261 taggatggcc aagcgcgatc gccccgccat cggggttgac cttgtccgta tcgaggccca
     7321 gttccctgtt gaccgcaatc gcctgtgcag cgaaagcctc gttcgattca attacatcga
     7381 aatcagccag cgtcattcta gagcgccgaa gcaggttgcg aaccgcaggc accgggccaa
     7441 ttcccataat tcccggttcc acgcccgagt gggaataagc tacgattcgt cctagtggcg
     7501 ttacgccagt ggtcctgaca tatttttccg acgcgatcac gactgccgcg gcgccgtcgt
     7561 tgatacccga cgcatttcct gcggtgacgc tgccttcttt cttgaaggcc ggcttcaggg
     7621 ccgccaggct gtccgccgtc gtgccggact tgggatattc gtctgtatcg aatgtcacca
     7681 ggcttcgacc ggatgctacc tcgacaggta caatctgcga tttgaaatgc ccggcttcga
     7741 tagccgcgag cgctcgtcgc tggctctcgg ccgcgaatgc gtcctggtcc ttgcgtgaaa
     7801 cctgataatt ctcggcaacg ttttcagccg tcatccccat atggccattg ccgaatggat
     7861 cggtaagaac gccagtaagg ccgtccaaca tcgtgacgtc gcccatcttg tgtccgaagc
     7921 gggcgctctg gacgtaatgg ggggacctgc tcatgatttc caccccaccc gccaaggcca
     7981 catcgacgtc gccgagcata acggtttggg cggccgagat gatcgcttgc aggccactgc
     8041 cgcaaaggcg attgaccgtc atggccggcg cttccttcgg gatttcggcg cctatcgcgg
     8101 ccatccgtgc gacgtatgcg tcctcaggcg ccgtcgggat tacgttgccg aaaacgacat
     8161 gctccaccga cgacgggtca agtccggccc gccggatggc ctcttgcgcc aactttgcac
     8221 ctagacccga cggcgtctgc cgagaaagcg atccaccgaa cgagccgatt gcagcgcggg
     8281 cggccgagag aatgaaagtc gattccgtca tgacattcgt catccttcgt cagggattgc
     8341 ccgctccatg caaccggtca cggccgctac ggtcgaattc tttgcatcaa gggaattgca
     8401 catcgatcag catggggccg gtgcgcgaca gggcatcggc aaatgcagcg tcgagttgct
     8461 ccagcgtaga aaccgccacc gatggaacac cgaagccctc ggcgatccgg caccattcga
     8521 tggccggacg atcgagagac atcatgtcgc tggcggtttg gccaggctcc gcgccaacct
     8581 tggcgtattc ccccagcagg atcgcgtaag accggttgtt aagcagcaat gtggtaaccg
     8641 ggaggttctc gcgggcctgc gtccagagtc cctggatcgt atacgccgcc gaaccgtcgg
     8701 cttgcagcgt cacgacccgg cgatcgccgc gacccatggc tctggcgcca accgcggcgc
     8761 ccgttgccaa aggaatgccg ccgccgatgg ccccgcctgt gacggtgagc cagtcgcact
     8821 gttcagcatg aggcatcacg gcgctgaagc cgccgccgag agtgagcgcc tcattcacga
     8881 cgatggcgtc tttcggaagt cgccgtgcga ccgatgcggc gaaaccatac ggctctatcg
     8941 gtccgtgtgg ttcgggacta tccgacgatc gtgccggcac tgttgcaggc tgcaccgcaa
     9001 gctcgtctgc caggaatctc aaggcaaccg ccgcgtcctg atcttcccgt gaaagcacca
     9061 gcacattggc atcggggcga tgcagcaggc tcggacgtcc cggataaagg aaaaagcctg
     9121 tcggcgcagg acagttcacg agtacgaccg tgtcaaaaac cgcaagctcc gccaccgcgg
     9181 catcgaccgg atatggaacg cttttcagat cgagcctgcc acgccctctc ggcatgttcg
     9241 caatcgtcga gctgcccatg agagtgacgc ccgtggctgc cgctataccg gcaagaagcg
     9301 ggtggagatc ggctgcg
//
LOCUS       DASYCS010000231         5077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4260638,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000231 DASYCS010000000
VERSION     DASYCS010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 5077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5077
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4260638"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1513
                     /locus_tag="VGM46_07365"
     CDS             <1..1513
                     /locus_tag="VGM46_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HEY4192450.1"
                     /translation="LAKTAGPSGERSPEPPSFHNSYARLPDRFFAVQQPNPVSAPRLV
                     RVNGPLAADLGLDPQWLESPAGIDMLAGNRFPEGAEPIALAYAGHQFGNFTPQLGDGR
                     ALLVGEVIDSHGVRRDIQLKGSGPTRFSRNGDGRAALGPVLREYIVSEAMAALGIPTA
                     RTLAAVTTGDKVYRETRLPGAIVARVAASHVRIGTFQYFAARRDVEALKILTHYVIDR
                     HYPALASHDRPALALLEAVIAAQAALVARWMLVGFIHGVMNTDNMTVSGETIDYGPCA
                     FMDRYDPATVFSSIDVQGRYAYANQPRIGVWNLTRLAETLLPLLHDDEAEAIKIAEDA
                     LQAFQANYLAAFHEGLRRKLGLLTQEEGDLALAGGLLGLMAASRADFTTTFRNLASEI
                     DKPAGSEDGVRLLFADPEAFDGWATAWRQRLAREPDIGASRQAMLSANPKYIPRNHRV
                     EAAIAAAVERSDFGPFEELLAVTGKPFEEQPAMERYALPPEEEDRGFQTFCGT"
     gene            complement(1530..2039)
                     /locus_tag="VGM46_07370"
     CDS             complement(1530..2039)
                     /locus_tag="VGM46_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014531237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HEY4192451.1"
                     /translation="METPAPKTPAFDGEIGILQRRKIEAGIIAPIYEGMVREIGEARA
                     QAILDEAITRAAIDAGRSFAEKTEGGPNLLTFQELQALWTRDDALEVEVVRATAEHFD
                     YNVHRCRYAEAYKEMGLGKIGHLLSCNRDGVFCQGYDPRIKLKRTQTLMQGADHCDFR
                     YTFVDDGAA"
     gene            complement(2119..2745)
                     /locus_tag="VGM46_07375"
     CDS             complement(2119..2745)
                     /locus_tag="VGM46_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit gamma family protein"
                     /protein_id="HEY4192452.1"
                     /translation="MADFNWTARSPLQRLAVPGHYGVETRTPGIVLSEVRGFDLVLIL
                     ARRGQWAATAKAVGTHFGVAAPAVPRAVAGKAATIIWSGPDQVLALSPRQGTTPPLDR
                     LKPAFEGIASLSDQSDGRALIAISGPDARNMLAKVSSLDLHDGIFPVGAAAATSLDHT
                     AVTLWRATDAADGSPVFNLLVFTSFAESLWHTILDASLEYGVEVSEIG"
     gene            complement(2738..>5077)
                     /locus_tag="VGM46_07380"
     CDS             complement(2738..>5077)
                     /locus_tag="VGM46_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006330122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEY4192453.1"
                     /translation="GEIDGGNATAWLASVLTELKSLPNVRIMTRTSVFGVYDGGTYAA
                     LERISDHLTTPPPHRVRQRLWRIVARRSIVAAGAVERPIVFSGNDRPGVMMASAVRSY
                     VERFAAAPGKRMALFTNNDDGWKTIETAMRAGISIAAVIDSRGTLPDRYRALAQSAGF
                     SLHHGAVADVHGGAKGVTAIEIALTGGGKATVAADVLAMSGGWNPSVGLSSHHRGRPK
                     WSDDIAAFVPNDAPPGMTAAGAANGDFSLGACLRNGHQAGLLAAADCGHEGRAGNAPT
                     ASDEAVAITPLWHVAGKHKAFVDFQHDVTASDIALAQREGFESVEHLKRYTTLGMATD
                     QGKNSNVTGLAIMAELTKRSIPQTGTTIYRPPQVPVAIGALAGHHRDENFHATRLTPS
                     HHWAAAHGAVFVDTGLWKRAQWFPLAGENDWLTSVNREVLAVRSGVGFCDVSTLGKID
                     VMGPDAGIFLDRVYINAFSSLAVGKARYGLMLREDGIVMDDGTTSRLAQDHYFMTTTT
                     ANAGLVMQRLEFCRQVLWPELDVQLTSCTDQWAQFSIAGPKTRDLLRAIVDPAEDLSN
                     EGFPFMAAREVALRGGLRARLFRISFSGELAFEISVPARYGEAMAENLMIAGQPFGVI
                     PYGTETLGVMRIEKGHVAGAELNGTTTAIDLGLGKMASKKKDFIGRVMAGREALVAPD
                     RQVVVGLKPLDKARRLRSGAHIIPKGAAVGPDTDQGYVTSVCHSPVLSQWIGLGLVER
                     GRERIGETVRAHDPLRGEDYDVEICSPVFYDPEGGRQRG"
BASE COUNT          878 a         1602 c         1711 g          886 t
ORIGIN      
        1 ccttgcaaaa acagccgggc catcaggcga aaggtcgcct gaaccgccgt ccttccacaa
       61 cagctatgcc cggcttcccg accgtttctt tgcagtccag caaccgaacc cggtatcggc
      121 gccccggctc gttcgcgtga acgggccttt ggcggcggac ctgggcctcg atccgcaatg
      181 gctggaaagt ccggccggca tcgacatgct ggccggcaac cgttttcccg agggcgccga
      241 gccgatcgcg cttgcctatg cggggcatca gttcggcaat ttcacgccgc aactgggcga
      301 cggccgcgcg ctgctggtcg gcgaggtgat cgatagtcat ggcgtccggc gcgatatcca
      361 gctcaaaggt tcgggaccga cgcggttttc ccgcaatggc gacgggcgcg ccgcacttgg
      421 cccggtgctg cgcgaatata tcgtcagcga agcgatggcg gcgctcggca ttcccaccgc
      481 ccgcacgctt gcggcggtaa ccaccggcga taaggtctat cgcgagacgc ggctgccggg
      541 cgccatcgtc gcccgcgtgg cggccagcca tgtgcggatc gggacgttcc aatatttcgc
      601 ggcccgccgc gacgtggaag cgctgaaaat cctgacgcat tacgtgatcg accgccacta
      661 tccggctctt gcttcgcatg accggccggc gctcgccttg cttgaggcgg ttatcgcggc
      721 ccaagccgcg ctggtggcgc gctggatgct ggtcggcttc attcacggcg tgatgaacac
      781 cgataatatg acggtttccg gcgagaccat cgactacggt ccctgcgcgt tcatggatcg
      841 gtatgacccg gcgaccgtgt tcagttcgat cgacgtgcag gggcgctatg cctatgccaa
      901 ccagccgcgc atcggggtgt ggaacctgac gcggcttgcc gaaaccctgc tgcccttgct
      961 gcatgacgac gaggccgagg cgatcaagat cgccgaagac gcgctacagg cgttccaggc
     1021 caattatctt gcggcgtttc acgaagggtt gcgccgaaag ctcgggctgt tgacgcagga
     1081 ggaaggcgac ctggcgctgg ccggcggcct gcttggcctg atggcggcaa gccgcgccga
     1141 cttcaccacg accttccgca atctggcctc agaaatcgac aagccggcgg ggagcgaaga
     1201 tggcgtgcgc ctgctgtttg ccgacccgga ggcgttcgac ggctgggcca cggcgtggcg
     1261 gcagcggctt gcaagggagc cggatatcgg tgcgagccgc caggccatgc tgtcggccaa
     1321 tccgaaatac attccccgca accatcgcgt cgaggccgcc atcgcggctg cggtggagcg
     1381 ttcggatttc ggcccgttcg aggaattgct ggcggtgacg ggcaagccgt tcgaggagca
     1441 gccggccatg gaacgctacg cgctgccgcc ggaagaagag gaccggggtt tccagacttt
     1501 ctgcgggacg tgagaagaag gcgaagcttt caggccgcgc cgtcgtccac gaacgtatag
     1561 cggaaatcgc agtggtcggc gccctgcatg agcgtctgcg tgcgtttcaa tttgatgcga
     1621 gggtcgtagc cttggcaaaa aacgccgtcg cggttgcagg acaagaggtg gccgatcttg
     1681 ccgaggccca tttccttata ggcttcggca taacggcagc ggtggacatt atagtcgaaa
     1741 tgctccgccg tcgcgcgaac cacttccacc tcaagggcgt cgtccctggt ccacagcgcc
     1801 tgcaattcct ggaaggtgag aaggttcggc ccgccttcgg tcttttccgc gaaagatcgt
     1861 ccggcatcga ttgccgccct ggtgatggcc tcgtcgagga tcgcctgcgc ccgcgcttcg
     1921 ccgatctcgc gcaccatgcc ctcatagatg ggggcgatga tgccggcctc gatcttgcgg
     1981 cgttgcagaa tgccgatctc gccgtcgaac gccggggttt ttggggcggg agtttccata
     2041 agcgtctggc tccgtaagtc acggcccgtc gattgcatgg ccgatacggc atctatgtag
     2101 ggaggccggc tgaaaagatc acccgatttc cgaaacctcg acgccatatt cgagcgatgc
     2161 atcgaggatc gtgtgccaga gactttcggc aaagctcgta aacaccagca ggttgaaaac
     2221 cggcgatcca tcggcggcgt cggtggcgcg ccacagggtg accgcggtgt gatcgagcga
     2281 cgtcgccgca gccgcgccga ccgggaagat gccgtcatgc aggtcgagcg acgacacctt
     2341 ggccagcatg ttgcgggcgt cggggccgga aatggcgata agcgcgcggc cgtccgactg
     2401 gtcggacaat gaggcaatgc cttcgaaagc aggcttcagc cgatccagcg gcggcgtcgt
     2461 gccctggcgc ggcgacagcg ccagaacctg atccgggccg gaccagatga ttgtcgctgc
     2521 cttgccggcg accgccctcg gcacagccgg cgcggcaacg ccgaaatggg tgccgaccgc
     2581 cttggcggtt gcggcccact ggccgcgccg ggcgaggatc agcacgaggt cgaaaccgcg
     2641 cacttccgac aagacgatgc cgggcgtccg tgtctccacg ccataatggc cgggaacggc
     2701 aagccgttgc aggggcgagc gggctgtcca gttgaaatca gccacgctgg cgccctcctt
     2761 cgggatcgta gaataccggg ctgcaaatct cgacatcgta gtcttcgccg cgcagtggat
     2821 cgtgcgcgcg aacggtttcg ccgatgcgct cgcgcccacg ctcgactagc ccgaggccga
     2881 tccattggct tagaacaggc gaatggcaga cggaggtgac gtaaccctgg tcggtatcgg
     2941 gaccaaccgc cgcgcctttg gggatgatat gcgcgccgga tcgcagccgc cgtgccttgt
     3001 cgagcggctt cagcccgacc acgacctggc ggtccggcgc gaccagcgcc tcgcgcccgg
     3061 ccatcacgcg gccgatgaaa tccttcttct tcgaagccat cttgccgagg ccgagatcga
     3121 tggcggtggt ggtgccgttg agttcggcgc cggcgacatg gcctttctcg atacgcatga
     3181 cgcccagcgt ctcggtgccg taggggatga cgccgaaggg ttggccggca atcatcaggt
     3241 tttccgccat cgcttcgcca tagcgggcgg gaacggaaat ctcgaaggcc agctcgccgg
     3301 agaacgagat gcgaaacagc cttgcccgca ggccgccgcg cagggcgacc tcacgcgcgg
     3361 ccatgaacgg aaaaccttcg ttcgacaaat cttccgcagg atcgacaatg gcgcgcagca
     3421 ggtcgcgcgt cttcggtccg gcgatggaga attgtgccca ctggtcggtg caggaggtca
     3481 attgcacgtc gagttcgggc cacagcacct gccggcagaa ttccaggcgc tgcatcacca
     3541 gaccggcatt ggcggtggtg gtggtcatga aataatgatc ttgcgcaagc cgcgacgtgg
     3601 tgccgtcgtc catgacgatg ccgtcttcgc gcagcatcag cccatagcgt gccttgccga
     3661 cggcgagcga cgagaaggcg ttgatgtaga cgcggtcgag gaaaatgccg gcatccggtc
     3721 ccatcacgtc gatcttgccc agcgtcgaga catcgcagaa gccgacgccg ctacgcacgg
     3781 ccagcacctc gcggttgacg gaggtcagcc agtcgttttc gccggccagc ggaaaccact
     3841 gcgcgcgctt ccacaggccg gtgtcgacga acaccgcgcc gtgcgctgcc gcccagtggt
     3901 gcgaaggggt gagccgcgtt gcgtggaagt tttcgtcgcg gtgatggccg gccagcgcgc
     3961 cgatcgccac cggcacctgc ggcgggcggt agatggtggt gccggtctgc gggatcgagc
     4021 gcttggtcaa ttcggccatg atcgccaggc cggtgacgtt ggaattcttg ccctggtcgg
     4081 tcgccatgcc aagcgtggtg tagcgcttca gatgctcgac cgattcgaaa ccctcgcgct
     4141 gcgccaaggc gatgtcggag gcggtcacgt cgtgctggaa gtcgacgaag gccttgtgct
     4201 tgcccgcgac atgccagagc ggcgttatgg cgacggcttc gtcgcttgcg gtaggcgcat
     4261 ttccagcgcg gccctcgtgg ccgcaatcgg ctgccgccag aaggccggcc tgatggccgt
     4321 tgcgcaggca ggcaccgagc gaaaagtcgc cattggcggc gcctgccgcc gtcattccgg
     4381 gaggcgcgtc gttgggcacg aaagcggcga tgtcgtcgct ccatttcgga cgcccgcgat
     4441 gatgcgagga caggccgacc gaggggttcc agccgccgga catggcaagc acatcggcgg
     4501 cgacggtcgc cttgccgccg ccggtcagcg cgatttcgat ggccgtcacg ccctttgcgc
     4561 cgccgtgaac atcggcaacc gcgccatgat ggagggaaaa gccggcgctt tgcgccagcg
     4621 cgcggtagcg gtccggcagg gtgccgcgcg aatcgattac cgccgcaatg cttatgccgg
     4681 cgcgcatggc ggtttcgatg gtcttccagc catcgtcatt gttggtgaac aaggccatcc
     4741 gcttgccggg ggccgccgcg aagcgttcga catagctgcg cacggcggac gccatcatca
     4801 cgccggggcg atcgttgccg gaaaagacga tcggccgttc gaccgcgccc gccgcgacga
     4861 tgctgcgcct ggcgacgatg cgccatagcc gctggcgaac ccgatgtggc ggcggggttg
     4921 ttaggtgatc gctgatgcgt tccagcgcag cgtaagtacc accgtcatag acgccgaaga
     4981 cgctggtgcg cgtcatgatg cggacattgg gcagcgattt cagttcagtc agcaccgagg
     5041 cgagccaggc ggttgcgtta ccaccgtcga tttcgcc
//
LOCUS       DASYCS010000232         9879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1654403,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000232 DASYCS010000000
VERSION     DASYCS010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 9879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9879
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1654403"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /gene="tsaB"
                     /locus_tag="VGM46_07385"
     CDS             complement(<1..383)
                     /gene="tsaB"
                     /locus_tag="VGM46_07385"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010913304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEY4192454.1"
                     /translation="MILAIDCSASLCAASVHDEAANVELGRCVLDLGKGHAERLMAVI
                     ADALKAGGVNYAGLSTIAVSVGPGSFTGVRVGVSAARGLALALKIPAIGVTTLEALAQ
                     QARQAFPGRAVLVALDGGRDEIHAAL"
     gene            complement(390..2567)
                     /locus_tag="VGM46_07390"
     CDS             complement(390..2567)
                     /locus_tag="VGM46_07390"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006326291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HEY4192455.1"
                     /translation="MTDRIDAAGLQVARELHDFLANEALPGTGIAADAFWRGFSAIAH
                     DLAPKNRALLATRDAMQAKLDDWYRENGAPIDMEVYKRFLSEIGYLVPEGPAFSVTTK
                     NVDPEIGVVAGPQLVVPVMNARYALNAANARWGSLYDALYGTDAISEADGAQKGEAFN
                     LKRGEKVIAWAKAFLDESAPLTTGKWAGVSGFSIANGMLRLSAGSGSTTLADPKQFVG
                     YRGDAANPDAVLLVRNGLHVEIVIDRNNRIGKTDPAGIADMVLESALTTIQDCEDSIA
                     AVDAEDKTIVYRNWLGLMRGDLAEQVEKGGKTFTRTLNGDRAYTARDGGELTLPGRSL
                     MLVRNVGHLMTNPAILLSDGSEIPEGIMDAVITALIALHDIGPNGARRNSRAGSMYVV
                     KPKMHGPDEVAFAVEIFERVEKLLGLAQNAIKMGIMDEERRTTVNLKEAIRSAKDRVA
                     FINTGFLDRTGDEIHTSMEAGPMIRKADMKNAAWISAYEDWNVDTGLECGLVGHAQIG
                     KGMWAMPDMMAAMLAQKIAHPKAGANTAWVPSPTAATLHATHYHKVDVQAVQEKLKNR
                     PKARLENILSVPIATRPNWSPEDIQAELDNNAQGILGYVVRWIDQGVGCSKVLDIHDV
                     GLMEDRATLRISSQHIANWLRHKVCTEEQVIETMKRMAAVVDRQNAGDPFYRPMAVDF
                     EGSIAFQAACDLVFRGREQTNGYTEPFLHARRLELKARENSAS"
     gene            complement(2714..4741)
                     /locus_tag="VGM46_07395"
     CDS             complement(2714..4741)
                     /locus_tag="VGM46_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006326292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEY4192456.1"
                     /translation="MNARSKSSPLFLGIDTGGTYTDAVLWSGEGGDRGTVHAKAKSLT
                     TRHDLAVGISGAVDAVLQRAGTDPADIKLVSMSTTLATNALVEGQGGRVALVMIGFGE
                     ADLDRDGLRKALGSDPVLFVPGGHDVHGNTAELDLSGLEAALPELEKSVSGFAVCAYF
                     ATRNPAHELAARDLIREKTGMPVTASHELSAKLGGPRRALTTLLNARLIAMIDRLVAA
                     TEGFLEARSITAPLMVVRGDGALVSAAFARQRPIETILSGPAASLVGARHMTGLDNAV
                     VSDIGGTTTDVAVLDRGRPRLDSEGATVGGYRTMVEAVAMRTFGLGGDSEVSLQSGAL
                     DPKIQLGPRRLVPLSLAGMMHGAAVTAELERQLREPNAGRMSGRFAMRTGVPERLAAG
                     LSSAEARLYEAIGTVPLALDKLLISNAQNATLTRLVARGLVHVCGFTPSDAAHTLGLQ
                     ANWDAGAARLGAELFARKRDGRGQFIAASPEALSQRVLAALTRFSAEAILETAFAEDG
                     LDGASTVAHALVQRAVDAQEGIARFSVALDRPVIGLGASAPLHYAGLPPLVGNECVVP
                     EDTDVANALGAVVGQVRVTVEARVSQPREGLFRLSSGETVRDFREEEPAIAAAREDVR
                     AIAASRAKEAGTDNFEIEVTCEYHVSTVQDQRMFIEAHIVAVASGRPRIAV"
     gene            complement(4851..5252)
                     /locus_tag="VGM46_07400"
     CDS             complement(4851..5252)
                     /locus_tag="VGM46_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006201105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192457.1"
                     /translation="MTRFSAFSLRLPGSVPTILLGLLLAVGLAAPSFAAEMNFYMKNQ
                     QDRGVAVELFSQSRSKVWPGGDKVYFLDSREKKSVPVTCEEGERICYGAWVNGDDTIS
                     FGVGPDNDKPPCEYCCFTCAPKSTQVISITK"
     gene            5472..5620
                     /locus_tag="VGM46_07405"
                     /pseudo
     CDS             5472..5620
                     /locus_tag="VGM46_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004687035.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
     gene            complement(5658..5852)
                     /locus_tag="VGM46_07410"
     CDS             complement(5658..5852)
                     /locus_tag="VGM46_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HEY4192458.1"
                     /translation="MTPKFEYDPEANAAYIRFSSEKVLESEEVSDGIILDYDIEGHIV
                     GMEVLDARQNLPRTMLEQAA"
     gene            complement(6041..7855)
                     /gene="lepA"
                     /locus_tag="VGM46_07415"
     CDS             complement(6041..7855)
                     /gene="lepA"
                     /locus_tag="VGM46_07415"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004689166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEY4192459.1"
                     /translation="MSTPISHIRNFSIVAHIDHGKSTLADRLIQLTGGMDAREMAGKE
                     QVLDSMDIERERGITIKAQTVRLKYHANNGEDYILNLIDTPGHVDFAYEVSRSLAACE
                     GSLLVVDASQGVEAQTLANVYQALDNNHEIVVVLNKIDLPAAEPERIREQVEEVIGID
                     ASNAVLISAKSGIGIPEVLEAIVNQLPPPREGDASAPLKAMLVDSWYDAYLGVIVLVR
                     VIDGVLKKGQTIRMMGTGAKYLVERTGVFTPARVAVDELGPGEFGFITGSIKEVADTR
                     VGDTITEDRRPTAKALPGFKPAQPVVFCGLFPVDAADFEDLRAAVGKLRLNDASFSYE
                     METSAALGFGFRCGFLGLLHLEIIQERLEREFDLDLIATAPSVVYRLNLSDGTQKELH
                     NPADMPDVMKITSIEEPWIRATILTPDDYLGGTLKLCQDRRGIQADLSYVGKRAMLVY
                     DLPLNEVVFDFYDRLKSISKGYASFDYHLTDYREGDLVKMSILVNEEPVDALSMLVHR
                     TAAEKRGRVMCEKLKELIPNHLFKIPIQAAIGGKVIARETISALRKDVTAKCYGGDVS
                     RKRKLLDKQKEGKKRMRQFGKVDIPQEAFIQALKMGDN"
     gene            complement(7922..8557)
                     /locus_tag="VGM46_07420"
     CDS             complement(7922..8557)
                     /locus_tag="VGM46_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrsF family protein"
                     /protein_id="HEY4192460.1"
                     /translation="MQTNDLIEILAKDARATAPSPVMVLAGAAVVAIMVAAAVFFSVL
                     GPRPDFALAMSTTRFALKFVVTGALVVTALALAARLARPGAGLGAMNWLLLAPLILLV
                     AVLGEFRAIPSADWHTRWIGTNSLVCMMSIPLIGVGPLAVFLVAIRYGAPTRPRLAGA
                     VAGLLAGGLAALFYAAHCPDDSPFFVATWYTIAIAGMAALGALAGQRLLRW"
     gene            complement(8559..9095)
                     /locus_tag="VGM46_07425"
     CDS             complement(8559..9095)
                     /locus_tag="VGM46_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018329922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY4192461.1"
                     /translation="MRGAGEAELAVLLKAAIAGDERAYAGFLADIASRVRGFARRRLQ
                     GGGVDTEDIVQETLLAIHLKRHTWRQDAAVLPWVFAIARFKIIDAFRRRGRAVEVDIS
                     DFSETLAEPEKEAVSSRDISRVVETLAPAQKAVVSAISVEGRSIADTAKALDMTEAAV
                     RVAFHRGLASIAARFGKN"
     gene            9330..9629
                     /locus_tag="VGM46_07430"
     CDS             9330..9629
                     /locus_tag="VGM46_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006326330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192462.1"
                     /translation="MKNFTRMTLPALAFALASTALFAGGAAHAEDAMKPANAMKAGDA
                     MKPADAMKPADAMKPADAMKPADAMKPADAMKPADAMKPADAMKPADAMKPAKAN"
BASE COUNT         1833 a         3166 c         3124 g         1756 t
ORIGIN      
        1 agcgcggcat ggatttcgtc acgcccgccg tcgagcgcca ccagcaccgc acggcctgga
       61 aaagcctgcc gcgcctgttg cgccagcgct tccagcgtgg tcacgccgat cgccggtatc
      121 ttcagcgcca gcgccagccc tcgcgcggcg gaaacgccga cgcgaacgcc ggtgaaggag
      181 ccggggccaa ccgaaaccgc gatggtcgaa agcccggcat agttgacgcc gcccgccttc
      241 aacgcatcgg cgatgaccgc catcaggcgc tccgcatggc ccttgccgag gtcgagcacg
      301 caacggccaa gctcgacgtt ggctgcctcg tcatgaacgc tggccgcgca caggctggcg
      361 gaacaatcga tggcgaggat catgaccgtt tacgaggcac tattctccct cgccttcagt
      421 tccagccgcc gcgcgtgcag gaacggctcg gtatagccgt tggtctgctc gcggccccgg
      481 aagacgagat cgcaggcggc ctggaaggca atggagcctt cgaaatccac cgccatcggc
      541 cggtagaacg gatcgcccgc attctggcgg tcgaccacgg cggccatgcg cttcatcgtc
      601 tctatcacct gctcttcggt acacacctta tggcgcagcc agttggcgat gtgctgcgag
      661 gaaatgcgca gcgtggcgcg gtcttccatc agcccgacat cgtgaatgtc cagcaccttg
      721 gagcagccga cgccctggtc gatccagcgc acgacatagc cgaggatgcc ttgcgcatta
      781 ttgtccagtt ccgcctggat gtcttccggc gaccagttcg ggcgcgtcgc tatcggcacc
      841 gacaggatgt tttcgagcct ggccttcggg cggttcttca gcttttcctg caccgcctgc
      901 acatcgacct tgtggtagtg cgtggcgtgc agcgtcgccg ccgtcggcga aggcacccat
      961 gccgtgttcg cgccggcctt ggggtgggcg atcttctgtg ccagcatggc ggccatcatg
     1021 tcgggcatcg cccacatgcc cttgccgatc tgggcgtggc cgacaaggcc gcattcaagg
     1081 ccggtatcga cgttccagtc ctcataggcg gaaatccagg cggcattctt catgtccgcc
     1141 ttgcggatca tcgggccggc ttccatcgag gtatggattt cgtcgccggt gcggtcgagg
     1201 aaaccggtgt tgatgaaggc gacgcggtcc ttggccgagc ggatggcttc cttgaggttg
     1261 acggtcgtgc gccgctcctc gtccatgatg cccatcttga tggcgttttg tgccaagccg
     1321 agcagttttt cgacgcgctc gaatatttcc accgcgaacg ccacttcgtc tggcccatgc
     1381 atcttcggct tgacgacata catcgagccg gcgcgcgaat ttctgcgcgc gccgttcggg
     1441 ccgatgtcgt gcagggcaat cagcgcggtg attaccgcat ccatgatgcc ttccggtatt
     1501 tcgctgccgt cagacaaaag gatcgccggg ttggtcatca ggtggccaac attgcgcacc
     1561 agcatcagcg aacggccggg caaggtcagt tcgccgccat cgcgcgcagt ataggcgcgg
     1621 tcgccgttca gcgtgcgcgt gaacgtcttg ccgcctttct cgacttgctc ggccagatcg
     1681 cccctcatca agccaagcca gttgcgatag acgatggtct tgtcctcggc atcgacggcg
     1741 gcgatggaat cctcgcagtc ctgaatggtg gtcagcgccg attccagcac catgtcggcg
     1801 atgccggcag ggtctgtttt tccgatcctg ttgttccggt cgatgacgat ctcgacatgc
     1861 aaaccattcc tgaccagcag gaccgcgtcc gggttggcgg catcgccgcg atagccgacg
     1921 aactgcttcg ggtcggcaag ggtcgtcgag ccgctgccgg cgctcagccg cagcattcca
     1981 ttggcaatcg aaaagccgct gacgccggcc catttgccgg tggtgagcgg ggcgctttcg
     2041 tccaggaacg ccttggccca ggcgatgacc ttttcgccgc gcttcaggtt gaacgcctcg
     2101 cccttctgcg ccccgtccgc ctcggaaatg gcatcggtgc catagagcgc gtcgtagagc
     2161 gaaccccagc gcgcattggc ggcattgagc gcataacgcg cgttcatgac cggcaccacc
     2221 agttgcgggc cggcgacgac gccaatctcg ggatcgacat tcttcgtcgt gacgctgaag
     2281 gccgggcctt cggggaccag atagccgatc tcgctcagga agcgcttgta gacttccatg
     2341 tcgatgggcg cgccattttc gcgataccaa tcgtcaagct ttgcctgcat cgcgtcacgc
     2401 gtggccagca gcgcacggtt ctttggcgcc agatcatggg cgatggctga aaatcctcgc
     2461 cagaaagcat cggcggcgat gcccgtgccc ggcagcgctt cgttggcgag aaaatcgtga
     2521 agttcccgcg cgacctgcaa tccggcagcg tcaatccgat cagtcatcga tattctccca
     2581 ggtcttccaa ttagtgcggc atggaattgg cggacgtttg gcatgtacgg caggaggggt
     2641 caattcgccg aaaggccccg gagaggccgc ttgcaccgcg cgaaaaacca tttttcaccg
     2701 ctccttgcgc gatctacacg gcaattcgcg gccggcccga agcgaccgcc acgatatgcg
     2761 cctcgatgaa catgcgctga tcctgcaccg tcgagacatg gtattcgcag gtcacctcga
     2821 tctcgaaatt gtcggtgccg gcttccttcg cgcggctggc ggcaatggcg cgcacgtctt
     2881 ccctggcggc ggcgatcgcc ggctcctcct cgcggaaatc acggaccgtt tcacccgacg
     2941 agagccggaa caggccttcc ctgggctggc tgacgcgcgc ttcgaccgtg acgcgaacct
     3001 gaccgacgac agcgcccagc gcgttggcga catcggtatc ttccggcacc acgcattcat
     3061 tgccgacaag cggcggcagc ccggcgtaat gcagcggcgc ggaagcgcca aggccgatga
     3121 cggggcggtc gagcgccact gaaaagcgtg cgatgccctc ttgtgcatcg acggcccgct
     3181 ggaccagcgc atgggcgacc gtcgaggcgc cgtccagccc gtcttcggcg aaggccgtct
     3241 ccaggatcgc ttcggcggaa aaccgcgtca gcgccgccag cacgcgttgc gacagcgctt
     3301 ccggcgaggc cgcgatgaat tgcccgcgcc cgtcgcgctt gcgggcgaac agttccgcgc
     3361 caagccgggc cgcgcccgca tcccaattgg cctgaaggcc aagcgtatgg gcggcatccg
     3421 atggcgtgaa gccgcagaca tgcaccagcc ctctcgccac cagccgggtc agcgtggcgt
     3481 tctgggcatt ggaaatcaac agcttgtcca gggccagcgg caccgtgccg atggcttcgt
     3541 aaagccgcgc ttcggcgctc gaaaggcctg ccgccagccg ctcgggcaca ccggtgcgca
     3601 tggcgaagcg cccgctcatg cggccggcat tcggctcgcg cagctggcgc tccagttcgg
     3661 ccgtgacggc tgcgccatgc atcatgccgg caagcgaaag cggcaccagg cggcgtggcc
     3721 cgagctggat tttgggatcg agcgcgcccg attgcagcga cacttccgaa tcgccgccaa
     3781 ggccgaaggt gcgcatggcc accgcctcga ccatggtgcg gtagccgccg acggtggccc
     3841 cttccgagtc gaggcgcgga cgcccgcgat ccagcacggc gacatcggtg gtggtaccgc
     3901 caatgtcgga gaccaccgca ttgtcgaggc ctgtcatgtg gcgcgcgccg acaaggctgg
     3961 cggcggggcc ggaaaggatc gtctcgatcg gccgctggcg ggcgaacgcc gccgaaacca
     4021 gcgcgccatc accgcgcacc accatcagcg gcgcggtgat gctgcgggct tcgaggaagc
     4081 cttccgtcgc cgccaccagc cggtcgatca tggcgatcag cctggcgttc aaaagcgtcg
     4141 tcagcgcccg gcgcgggcca ccgagcttcg cggaaagttc gtggctggcg gtgaccggca
     4201 tgccggtctt ttcgcggata agatcgcgcg cggccagttc atgcgcgggg ttgcgggtcg
     4261 caaaataagc acagacggca aagcccgaca ccgacttttc cagttccggc agcgccgcct
     4321 caagaccgga aaggtcgagt tcggcggtat tgccgtggac gtcatggccg ccgggcacaa
     4381 acagcaccgg atcggaaccg agcgccttgc gcaggccgtc gcggtcgaga tcggcctcgc
     4441 cgaagccgat catcaccagc gcgacgcgcc cgccctgccc ctcgaccagc gcgttggtgg
     4501 cgagcgtggt cgacatcgac accagcttga tatccgccgg atcggtgccg gctctctgca
     4561 acaccgcatc gaccgcgccg gaaatgccga ccgcaaggtc gtggcgtgtc gtcagcgact
     4621 tcgccttggc atgaaccgtg cccctgtcgc ccccctcgcc cgaccacaaa acggcgtccg
     4681 tataggtgcc gcccgtgtct atgccgagga aaagcggcga cgacttcgat cttgcgttca
     4741 tttcagggca gtccggaaag ctgtgggcac gataggcgtg gccatagacc attggcggct
     4801 caaaaggaaa ccggccagcg ccgttctttc gtcatcccag cttcaacgct ctatttcgtg
     4861 atcgaaatta cctgtgtcga cttcggcgcg caggtaaagc agcaatattc gcatggcggc
     4921 ttgtcgttgt ccggcccgac gccgaaggag atggtgtcgt cgccattgac ccatgcgccg
     4981 taacagatgc gctcgccttc ctcgcaggtg accggcaccg acttcttctc gcggctatcg
     5041 aggaaataga ccttgtcgcc gcccggccag accttgctgc gcgactggct gaacagctcg
     5101 accgcgacgc cacgatcctg ctggttcttc atgtagaaat tcatctcggc ggcgaaggac
     5161 ggggcggcaa ggccgacggc gagcagaagc ccaagcaaga tcgtcggaac gctccccggc
     5221 agacgcaatg aaaacgcgga aaatcgtgtc atggcagttt cccgaatcat ccacgccaag
     5281 caaagcacga gcctatgcgc cgcgaaagcc gcccccgccc ttgtgcattc tctatttttt
     5341 tgcctcgcat agaggctcgc agtccgatct tctcagctta actccaaaaa agaggggagg
     5401 tgcgaacgga tttctgcgac tgactaaagg agcggatgag ttgccgtcac agaggacagg
     5461 ccttgcgcga caccgcaaaa cggcggccgg cggtggtggt attcaccgag accttctacc
     5521 ggatcaccga cgcaataggg cgcaagcatc agaccaagac atcgccacgg gtgaagaagg
     5581 aagcgccggg tgcattaaac gtgaggcttt gccgaagttg acagatggtg aaaaacgatc
     5641 cgggaaaggg aaacggctta agccgcctgc tccagcatgg ttcgcggcaa attctggcga
     5701 gcatcaagca cttccatgcc gacgatatgg ccctcgatgt cgtagtcgag aatgatcccg
     5761 tcggaaactt cttcgctttc gagcactttt tccgaggaaa accggatata agccgcattg
     5821 gcttccggat catattcaaa ttttggcgtc atgtagacct cgctctgcgg tcaaggaacg
     5881 ccgatattat acgcatttcg tcagccgttt ccaccacggc gattggcgcc gttgtaacgg
     5941 caccaatggg taggacctgt gcctgcggcg cctccaaccc gaacttgcgg tcgcatagac
     6001 ccgccgggtc tatgccttcc gcccaaaaag agcgaaccgc tcagttgtcg cccatcttga
     6061 gcgcctggat gaaggcttct tgcgggatgt ctaccttgcc gaactggcgc atgcgctttt
     6121 tgccctcttt ctgcttgtcg agcagcttgc gcttgcggga aacgtcgccg ccgtagcatt
     6181 tggcggtcac gtccttgcgc agggcggaaa tcgtctcgcg ggcgatgacc ttgccgccga
     6241 tggcggcctg gatcgggatc ttgaacagat ggttcgggat cagttccttc agcttctcgc
     6301 acatcacgcg gccgcgcttt tcggccgccg tgcggtggac cagcatggat agcgcgtcca
     6361 ccggctcctc gttgacgagg atcgacatct tgaccaggtc gccctcgcgg tagtcggtca
     6421 aatggtagtc gaaggaggcg tagcccttgg agatcgattt caaccggtcg tagaagtcga
     6481 acaccacctc gttcagcggc aggtcgtaga ccagcatggc gcgcttgccg acgtaggaaa
     6541 ggtcggcctg gatgccgcgc ctgtcctggc agagcttcag ggtgccgccg agataatcgt
     6601 cgggggtgag gatggtggcg cgtatccacg gctcctcgat cgaggtgatc ttcatcacgt
     6661 cgggcatatc ggcggggttg tgcagttcct tttgcgtgcc gtccgacagg ttcaggcggt
     6721 agacgacgct tggcgcggtg gcgatgaggt cgaggtcgaa ctcgcgctcc agccgctcct
     6781 ggatgatttc cagatgcagc agccccagaa acccgcagcg gaagccgaag ccgagtgccg
     6841 ccgaggtttc catttcatag gagaaggagg cgtcgttgag gcgcagcttg ccgacggcgg
     6901 cgcgcaaatc ctcgaaatcg gcggcatcga cagggaacag gccgcagaaa accaccggct
     6961 gcgccggctt gaagcccggc agcgccttgg cggtcgggcg tcggtcctcg gtgatggtgt
     7021 cgccgacgcg ggtatcggcc acttccttga tcgagccggt gatgaagccg aactcgccgg
     7081 ggccaagctc gtccaccgcg acgcgggcgg gggtgaacac gccggtgcgc tcgaccagat
     7141 atttggcgcc tgtgcccatc atgcggatgg tctggccctt cttgagcacg ccgtcgatga
     7201 cgcgcaccag gacgatgacg ccgagatagg cgtcgtacca gctatcgacc agcatcgcct
     7261 tcagcggagc gctcgcgtcg ccctcgcgcg gcggcggcag ctggttgacg atggcctcca
     7321 gcacctcggg gatgccgatg ccggatttgg ccgaaatcag cacggcgttg gaagcgtcga
     7381 tgccgatcac ctcctcgacc tgctcgcgaa tacgttccgg ttcggcggcg ggaaggtcga
     7441 tcttgttcag caccacgacg atctcgtggt tgttgtcgag ggcctggtag acattggcca
     7501 gcgtttgcgc ctcgacgccc tggctggcgt cgaccaccag cagcgagcct tcgcaggccg
     7561 ccagcgagcg cgacacctca taggcgaagt cgacatggcc gggcgtgtcg atgaggttga
     7621 ggatgtagtc ctcgccattg ttggcgtggt atttcaggcg caccgtctgc gccttgatgg
     7681 tgatgccgcg ctcgcgctcg atgtccatcg agtccagcac ctgttccttg ccggccattt
     7741 cgcgcgcgtc catgccgccg gtcagctgga tcagccggtc ggcaagcgtg gacttgccat
     7801 ggtcgatatg ggcgacgatg gagaagttgc ggatgtggga aatgggcgtg ctcatgccgc
     7861 ggcctttagc aggggcgggt tcatgcggca agcggcggcg cgacgcgcac cgcgccgatg
     7921 ttcaccagcg cagcaggcgc tggccggcca gagcgccgag cgcggccatt cccgcgattg
     7981 cgatcgtgta ccaggtggcg acgaagaaag gtgaatcgtc ggggcaatgc gccgcgtaaa
     8041 acagcgccgc gaggccgcct gccagcaggc cggcaacggc tccggcaagg cgcggacgcg
     8101 tcggcgcgcc atatctgatg gccaccagga acaccgccag cgggccgacg ccgatcagcg
     8161 ggatgctcat catgcagacg aggctgttgg ttccgatcca gcgcgtgtgc caatcggcgg
     8221 acggtatcgc ccggaattcg ccgagcacgg cgacaagcag gatcaacggg gcaagcagga
     8281 gccagttcat ggcgccgagg ccggcgcccg gccgggcaag gcgcgcggca agggccaggg
     8341 cggtaaccac cagcgcgccg gtgacgacga acttcagggc aaaccgcgtc gtcgacatgg
     8401 caagggcgaa atcggggcgt gggccaagga cgctgaaaaa cacggccgcc gcaaccatga
     8461 ttgccaccac ggccgcgccg gcaagcacca tgacgggcga aggtgccgtg gcgcgggcat
     8521 cctttgccag tatctcgata agatcgttcg tctgcatgtt agttctttcc gaatcgtgct
     8581 gcgatggagg ccagcccgcg atgaaacgcc acgcgaacgg ccgcctccgt catgtcgagc
     8641 gccttggcag tatcggcgat ggagcgccct tcgacggaga tcgccgacac caccgccttt
     8701 tgcgccggcg ccaaagtctc gaccacacgg gaaatgtcgc gagaactgac ggcctctttt
     8761 tccggttccg ccagcgtttc ggaaaaatcc gatatgtcga cctcgacggc acgcccgcgc
     8821 cggcggaaag catcgatgat cttgaaccgg gcaatcgcaa acacccacgg caagacggcg
     8881 gcatcctgcc gccatgtgtg gcgcttcaga tggattgcca acagcgtctc ctgcacgatg
     8941 tcctccgtgt cgacgccgcc gccctgcaac cgtcgacgcg caaaaccgcg cacccgcgac
     9001 gctatatcgg ccaggaaacc cgcataggcc cgctcgtcgc cggcaatcgc ggccttgagc
     9061 aaaacggcaa gttcggcttc gccagcgccc cgcacacagc actccccatt tattcggccg
     9121 ccgatgcctg ttgttacgga ctgcctgaaa aattgtccaa gcggaactcg atcacgaaac
     9181 tgtgtcgcgt cgcgtccgta acatcaaggg cgggccggcg aaataatgac acgagaggca
     9241 gcgaaccgcc ggctcgtatc gaatgtagca gccgcggcaa acggcaccgc attatgacgg
     9301 cggccaaagc caccagagga gagaaaacga tgaaaaactt cacccgcatg acgcttcccg
     9361 cactggcctt cgcgctggct tccaccgcac tttttgccgg cggcgccgcc catgccgaag
     9421 atgccatgaa gcccgcgaac gcaatgaagg cgggcgatgc catgaagcct gccgatgcca
     9481 tgaaacctgc cgatgcgatg aagccagcgg atgcgatgaa gccagcggat gcgatgaagc
     9541 ccgctgacgc catgaagccg gccgacgcca tgaagccagc ggatgcgatg aagccggccg
     9601 acgcgatgaa gcccgctaag gccaactaga tcttggtgtt tggctcggca tgaccggcct
     9661 gccggtcccg ccgggcgcgt ctgggactgg ctgtcgtttc cagcggacgg ctgcacacag
     9721 gccaagcaca tgaaattgag acgataatac cctatctatc gcgtaagatc gaaactctaa
     9781 ggatgctatg gagcccccaa ccatgactga acgaaggaat tcgataggaa aacgcctgcc
     9841 cggttcatct ggaaccgcgt tcgccgcgct ggcgctcgc
//
LOCUS       DASYCS010000233         2162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4551188,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000233 DASYCS010000000
VERSION     DASYCS010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4551188"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VGM46_07435"
     CDS             <1..612
                     /locus_tag="VGM46_07435"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY4192463.1"
                     /translation="VIYQFVKKASEVDLRITIGSPEQLIADIAKGGVDFAISPRIKLL
                     PGYSQAEFHHEINSLYCGDRHPLFGKKDPTREDVEAARFVVRPYANKAELQHFPNATV
                     GAYSSNIEAQAMYILSGHFLGYLPDHMTTMFRNNERLKALLVSESQIRSPFVIIAPKH
                     KKASLSQRIFIQELRKRAGEAGKATGKAPAVARAKPLPRLRSV"
     gene            complement(633..1265)
                     /locus_tag="VGM46_07440"
     CDS             complement(633..1265)
                     /locus_tag="VGM46_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HEY4192464.1"
                     /translation="MPTNASLGQLQLIPARKGVAVRVNRGQVVTVINTHGTQVVDSWA
                     FCADDVAEFMSMEHSRGAMLKVNPQNGDTLRSNKRRPMLTLIQDNSGGVHDTLIAACD
                     RYRYQQLGHVGHHDNCTDNLVAAMAPLGLKPPETPSPLNLFMNIPIGENGSVSFEAPV
                     SKAGSYVSMRAEMDLIVAFSACPQDLVPVNGADCVPTDAHFRIDERAPRL"
     gene            complement(1281..>2162)
                     /gene="speB"
                     /locus_tag="VGM46_07445"
     CDS             complement(1281..>2162)
                     /gene="speB"
                     /locus_tag="VGM46_07445"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEY4192465.1"
                     /translation="WDKIDADVAVLGVPFDMGTQYRSGARFGPRAIREASTLFSFGHG
                     GAYDHEDDETYLPLDKVRIVDVGDADIVHTDTATSHANTEKAVRKILERGALPLVLGG
                     DHAINIPCVRAFSEHGPIHIVQIDAHLDFVDVRHGVREGHGNPMRRAAEQKHVSGLSQ
                     IGIRNVSSTARDGYEDARQRGSTILSVRQARKLGLEGVLATIPAGARYYVTIDIDGFD
                     PSIAPGTGTPSHGGFLYYEVMEMLQALVKRGDVVGIDLVEVAPAYDPSGITGFLAAQV
                     LLNFLGYIFAEREARKR"
BASE COUNT          428 a          665 c          669 g          400 t
ORIGIN      
        1 gtcatctatc agttcgtcaa gaaggccagc gaggtcgacc tgcgcatcac catcggttcg
       61 cccgagcagc tgattgccga cattgcgaag ggcggcgtgg atttcgcgat ctcgccgcgc
      121 atcaagctgc tgccgggcta ttcgcaggcc gaattccacc atgagatcaa ttcgctctat
      181 tgcggcgacc gccatccgct gtttggaaag aaggacccga cgcgagagga tgtggaagcg
      241 gcccggttcg tcgttcggcc ctatgccaac aaggccgaac tacagcattt cccgaatgcc
      301 accgtcggcg cctattcgtc caatatcgaa gcgcaggcga tgtatattct aagcgggcac
      361 tttcttggct acctgcccga ccacatgacg accatgttca ggaacaacga gcggctcaag
      421 gcgctgctgg tttcagaatc gcagatccgc tcccctttcg tcatcatcgc ccccaagcac
      481 aaaaaggcgt cgctgtcgca gcgcattttc attcaggagt tgcgcaaaag ggcgggcgaa
      541 gcgggaaagg ccacggggaa agcccccgct gtcgcgcggg caaagccatt gcccagattg
      601 cgaagcgtgt agcggtttat ggattggatg ttctaaagac gcggcgcccg ctcgtcgatc
      661 ctgaaatgcg cgtcggtcgg aacgcagtcg gccccgttca ccggaaccag atcctgcggg
      721 catgccgaga acgcgacgat caaatccatt tccgcgcgca tcgagacata tgaacccgcc
      781 ttcgacaccg gcgcttcgaa tgaaaccgag ccgttttcgc cgatgggaat gttcatgaac
      841 aggtttagcg gcgaaggcgt ctccggcggc ttcaggccga gcggcgccat cgccgcgacc
      901 agattgtcgg tgcaattgtc atggtggccg acatggccaa gctgctggta gcgatagcgg
      961 tcgcaggcgg caatcagcgt gtcgtgcaca ccgccggaat tgtcctggat cagggtcagc
     1021 atcggacgcc gcttgttgct gcgcaacgta tcgccgtttt gcggattgac cttgagcatg
     1081 gcgccccggc tatgctccat cgacatgaac tccgccacgt cgtcggcgca gaaggcccag
     1141 ctgtcgacga cctgcgtgcc atgggtgttg atgaccgtaa ccacctgccc ccggttgacg
     1201 cgcaccgcta cgcctttgcg cgcgggaatg agttgaagct gtcccaggct ggcattcgtc
     1261 ggcatggcga tttctcccaa tcagcgtttg cgtgcttcac gctcggcgaa aatgtagccc
     1321 agaaagttca agagcacctg cgccgccaga aagccagtga tacccgaagg atcgtaagcc
     1381 ggggctactt cgacaaggtc gatgccgaca acgtcgccgc gcttgaccag agcctggagc
     1441 atttccatca cttcgtaata caggaagccg ccatggctgg gcgtaccggt gcccggcgca
     1501 atcgacggat cgaagccgtc aatgtcgatc gtcacataat agcgggcgcc cgccggaatg
     1561 gtcgccagca cgccctccag gccaagcttg cgcgcctggc ggacggagag gatggtcgag
     1621 ccgcgctggc gcgcatcctc gtagccgtcg cgggccgtgg aggaaacgtt gcggatgccg
     1681 atctggctaa gcccgctgac atgcttctgc tccgccgcgc ggcgcatcgg gttgccatgg
     1741 ccttcgcgca cgccatggcg cacatcgacg aagtcgagat gggcatcgat ctgcacgatg
     1801 tggatcggac catgctcgct gaacgcgcgg acgcagggaa tgttaatggc atggtcgccg
     1861 ccgagaacca gaggcagcgc gccgcgctca agtatcttgc ggaccgcctt ttccgtattc
     1921 gcatggctgg tggccgtatc ggtgtggacg atatcggcat cgccgacatc gacgatgcgc
     1981 accttgtcga gcggcaggta ggtctcgtcg tcctcatggt cataagcgcc gccatggccg
     2041 aaggaaaaca gcgtcgaggc ctcgcggatg gcgcgcgggc caaagcgcgc gcccgaacgg
     2101 tactgggtgc ccatgtcgaa aggcacgccg agcacggcga catcggcatc gatcttgtcc
     2161 ca
//
LOCUS       DASYCS010000234        19558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4983471,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000234 DASYCS010000000
VERSION     DASYCS010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 19558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19558
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4983471"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..917
                     /locus_tag="VGM46_07450"
     CDS             <1..917
                     /locus_tag="VGM46_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY4192466.1"
                     /translation="VAADCGRIVVVDNMVRGRIANLVSALPSGRVEIVNGDIRDQRLM
                     QTLVAGCDTVFHQAALRITHCAAEPDEAMQVMVDATYRLLRQCVEAKVRKVVMASSAS
                     IYGMAEDFPTTESHHPYGNRTLYGAAKSFGEGLLRSFNDMYGLDYVALRYFNVYGPRM
                     DIHGRYTEVMVRWMERIEAGEPPLIFGDGLQTMDLIHVTDVARANILAATAPASDTVF
                     NVGSGAETSLLGLARHFATAMGRPELQPEHREERAINPVPRRLSSTAAAREMLGFRSK
                     VEPQEGIADLVAWWRDEMKLARTTEMAS"
     gene            914..2083
                     /locus_tag="VGM46_07455"
     CDS             914..2083
                     /locus_tag="VGM46_07455"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004673256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEY4192467.1"
                     /translation="MIPIAKPLVGQEEADAASRVILSGWLTQGPQVAAFESEFAALVG
                     ADHACAVANCTVALHLALMALGVGPGDEVVTVSHTFIASANAIRQCGATPVFVDIEQD
                     GFNIDPALIGKAITPRTRAILCVHQIGMPCDMQAIMRIARAHGVPVIEDAACAIGSQI
                     RVDGQWQAVGRPLGDIVCFSFHPRKVLTVGDGGMLTTQNPEWDARFRLWRQHGMSVSD
                     AVRHGSREVILESYPVQGFNYRMTDIQAAVGREQLKRLPGIIASRRKRAEIYSAAIAG
                     IDGLTAPHEPDWALSNWQSYCVRLVPGLDQRAIMQDLLDHGVSTRRGIMCIHMEKAYA
                     DLPLPHSLRHSEKARDHSIILPLYAQMSHDEQMRVIELLAAAVARTSRTPHMAVA"
     gene            2097..3095
                     /locus_tag="VGM46_07460"
     CDS             2097..3095
                     /locus_tag="VGM46_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192468.1"
                     /translation="MPGPASQPRGSLNVMLQSIERRIGILHLDLTDLEVVTEAATGAY
                     ASTAVIAALAGAKVRACARDTHNHGSAEDAVAATRELARLAGVEDQIIFFHGVPPEVL
                     RSCDILTNTGRIRPITRNMVDLLQEDAVIALMFEAWEFRGSDLDLAACRERRIRIAAV
                     NERHGNVGVFPFLGPLCVCLLRDAGMNVAGKRIAVICDNPFATFLLAGLAEVGAEAAA
                     FESPLQLTPGPWDAVVLALLPQDDPLGQRHLEVIAERAPGALLAQFWGDVDRAAVKEL
                     GLRICPEIEPGRGHMGILLNRLGHEPIVRLQAGSLKAAEIVLRGGPFMPGGIASLL"
     gene            complement(3164..3703)
                     /locus_tag="VGM46_07465"
     CDS             complement(3164..3703)
                     /locus_tag="VGM46_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192469.1"
                     /translation="MAKLVDGVFNTRGGELEMVSGPPISRAILHKLEEIARRAKAHEI
                     TPEDAVKQAAEVAPVLGRIMGRFMVLGLPILTLLIALISLYLQYEGNRSSDEFESDAL
                     QLLERQVHAVELLAASANKEPENGVTKKSSGPTKVTPKKKPVVIKTPSKRRKNVNNAR
                     RQQHVEHKKSFPGRRRNPF"
     gene            3945..4445
                     /locus_tag="VGM46_07470"
     CDS             3945..4445
                     /locus_tag="VGM46_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006325189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcB family DNA repair protein"
                     /protein_id="HEY4192470.1"
                     /translation="MPIVSPILQNPLIDGRQSERAMLVRRGVQRLLMQMGAHVLPELS
                     LATGRRADLVALTRSGDIWIIEIKSSVEDFRVDRKWPDYRLHSDRFFFATHPGVPAEI
                     FPEECGFILSDGYGAEIMRDAPEHRMAAATRKALTLRIARAGAARLLAAELAGVSVPA
                     IEGESE"
     gene            complement(4535..5098)
                     /locus_tag="VGM46_07475"
     CDS             complement(4535..5098)
                     /locus_tag="VGM46_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015314185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ActR/PrrA/RegA family redox response regulator
                     transcription factor"
                     /protein_id="HEY4192471.1"
                     /translation="MNGEDAADGMVTGDDTSLLIVEDDKPFLTRLARAMETRGFTVET
                     AESVEEAVGKARANPPAYAVVDMRLGDGNGLDVVAAIREKRADARTVILTGYGNIATA
                     VTAVKLGAIDYLSKPADADDVFAALTRTSGERAAPPENPMSADRVRWEHIQRVYEMCE
                     RNVSETARRLNMHRRTLQRILAKRAPR"
     gene            complement(5193..6518)
                     /locus_tag="VGM46_07480"
     CDS             complement(5193..6518)
                     /locus_tag="VGM46_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ActS/PrrB/RegB family redox-sensitive histidine
                     kinase"
                     /protein_id="HEY4192472.1"
                     /translation="MIDFHRAPDFRQSQRLRLNTLIRLRWLAIVGQSITVIVVAYGLQ
                     FPLPVGVCFALIACSAWLNLILAFRYPAAHRLKPPAAMGILVFDSLQLAGLLYMTGGL
                     TNPFSLLMTVPVVISAASLPLRLTAALGGMVMALATLLVFFHLPLPWQAGEPLTLPFV
                     YVAGTWVAVFSSISFTAIYAFRVAEEARLLANALAATELVLQREQHLSALDGLAAAAA
                     HELGTPLATITLVAREMEKALGSDPKYREDVTLLRSQSERCREILKRLTSLSSEGEPQ
                     LSQLPLTSLVEEVIAPHRDFGISIALEPGARIGPEPVGRRNPGVLYGLGNLVENAVDF
                     AQAKVIIRWNWDDNHVNFSIADDGPGFPAEIIDRIGEPYMSTRQGAEPGGGLGLGLFI
                     AKTLLERSGASIAFSNSNGPGEGAIVEINWPRANFVNRHPAETDMFDTA"
     gene            complement(6637..9189)
                     /gene="hrpB"
                     /locus_tag="VGM46_07485"
     CDS             complement(6637..9189)
                     /gene="hrpB"
                     /locus_tag="VGM46_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015314187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HEY4192473.1"
                     /translation="MTKKDLPDLPISAVLPALGEALAAGNCAVLVAPPGAGKTTLAPL
                     ALLSAPWRGDGKLVLLEPRRLAARAAARRMAALLGEEPGETVGYAMRMENRTSACTRI
                     LVVTEGVFARMLLDDPELPGIAAVLFDEFHEHSLDGDFGLALALDVQSALRPDLRLLV
                     MSATLDGARVASLLGDAPVIESQGRAFPVDIRYEERPAGTPVEDAMAKAIRDALANET
                     GSILAFLPGQREILRTAERLEGRIGSDTDIVPLYGLLDGKAQDAAIKPSPAGRRKVVL
                     ATSIAETSITIDGVRVVIDSGLARLPRFEPASGLTRLETVRAAKASADQRAGRAGRTQ
                     PGVAIRLWRAEQTAALPAFTPPEILEADLSGLVLDCAAFGVADPTTLSFLDPPPAPAL
                     AEARALLKALGAIDDAGRLTKQGEAMRGLALPVRLAHMVAEAAKDGDAPSAQAAAELA
                     VLLTERGLGGDGVDLERRLMRFHGEHSPRAASARQLAQRLARNALASGSGKHGGRIDR
                     QDAKSTLAPVPRASPSRNGEEGKAGALLLHAWPDRVAKARGERGRFVLANGSGAALDA
                     ADPLAGQAFLVVADLTGKAQNARITAAAAVDEADIRAALGDRIEARRETLFDREKRAV
                     RVRETLRLGAITLAERMLPAPSGADADRAIIEAVREHGLSLLPWSKEAETLRRRLSFL
                     HRGLGAPWPDVSDAALIENLETWLEPFLTGAASFSAIDAAALSAGLASLVPHELQRKV
                     EALAPTHFDAPSGSRVPIRYDEEGPVLAIRVQELFGFDRHPSIAAGTVPLTLELLSPA
                     HRPIQTTRDLPGFWRGSWADVRADMRGRYPKHVWPENPLAATATARAKPRGS"
     gene            complement(9281..9826)
                     /locus_tag="VGM46_07490"
     CDS             complement(9281..9826)
                     /locus_tag="VGM46_07490"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEY4192474.1"
                     /translation="MNLTAFFSRPALAVAKDLIGATILVDGVGGVIVETEAYQPDDPA
                     SHAYRGPTNRNRAMFGPPACAYVYRSYGIHWCLNFVCLPGSAALIRALEPTAGLDAMA
                     PRRNTENPRLLCSGPGRLCQALGVDISMNGLALDKPPFSLKLAGEEMPVATGTRIGLS
                     RAVDWPWRFGLAGSPFVSRRF"
     gene            complement(9917..12661)
                     /locus_tag="VGM46_07495"
     CDS             complement(9917..12661)
                     /locus_tag="VGM46_07495"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY4192475.1"
                     /translation="MSQQFIIAAAVAYLALLFAVAYLVEHSSGRISRLTGSSVVYTLS
                     LAVYCSSWTFNGGVGRASQSGFDFLPIYLGPTLAFLLGPVLIRKILRVSQANRITSIA
                     DLIGARFNSGGLAALVTVIAVVGLVPYIALQLKSISSGFAALSQSGEAAHPMVQPLLA
                     DGALWAAVVLTIFAMLFGSRSIHPGEHHPGMVVAVALESILKFVCLTAIGLFVGYGLF
                     HGFGDLFAQAMAQPEIHSIFEYTPHRYGYDWIAMTLLAMMSVFCLPRQFQVMVVENVD
                     ERHLNRALWLFPFYLLAINLFILPIAIAGRMLLPVDTDPDNLVLSLPLATGHPVLALI
                     AFLGGLSASASMVVVEATALSLMVCNDVVMPALLRVKALGLAKKPDLTRLLLWVRRIV
                     MIAVMALGYLYMRHDSAQFALVSIGLMSFVAVAQFAPAIFGGLFWRGATKAGAFAGIS
                     AGVLVWFYTLLLPSFARSGALEIGFVADGPFGAALLKPYALFGLTGLDPVTHSAFWSL
                     LVNVGAFLIVSLMGHQSPVEQARAALFVDTEHLDEVAQIWRRTARVPDLMSLVSRFLG
                     REKAAAAFANWAHMRGLDPDATGQADAETVLFYERLLAGVIGAASARVLVSAIVEEEQ
                     LGVDEMMRILDETSRIIEANRQLEDKSRALEAATTELTEANAQLQVLDRLKDDFVATV
                     NHELRTPLASIRAFCEILRDHPDLPGEERREFLRIVVTESERLTRLINQLLDLSKIEA
                     TGAFPAQSAVVDLTAVIRESTAAMHQVFAAGRIELALRIEADPALVMGDHDRLVQVVI
                     NLLGNAAKFSQQGGGRATLHLARSGDEFEISVSDNGPGIHADDREIVFERFRQLGDTM
                     SGKPHGAGLGLAISQRIIAQHGGRIWVEDAEDGGAAFRIRLSPATVSMPGESLLAGAS
                     "
     gene            complement(12658..12888)
                     /locus_tag="VGM46_07500"
     CDS             complement(12658..12888)
                     /locus_tag="VGM46_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192476.1"
                     /translation="MLRRTTKRDRLVGLFIAAFVLFNPPILNLVSGTLFGWPALYIYL
                     FGVWAALIAATALIVERGGNPAAGRDRDTKRP"
     gene            complement(12899..13300)
                     /locus_tag="VGM46_07505"
     CDS             complement(12899..13300)
                     /locus_tag="VGM46_07505"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY4192477.1"
                     /translation="MSASILIADDDPNILLALTYLMRREGHDVRTATDGQEALDAVAQ
                     AVPDLVLLDLMMPKANGYDVCRMLRASPAYDGVRIIMLTAKGREADRRAGLALGADAY
                     ISKPFAIGDVVTCVASVLAEKPQLSTAISDS"
     gene            13406..14170
                     /locus_tag="VGM46_07510"
     CDS             13406..14170
                     /locus_tag="VGM46_07510"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY4192478.1"
                     /translation="MNGAGRLSSGTVSDGSDGLPGISQPRILVVDDDRDIAGLLRREL
                     VSLGFTVDSVEFAGDALLAARDVAYALMIVDLGLPDRDGLDLVRDMRSRLIEAPILVL
                     TARNGVEDRVTGLSCGADDYLVKPFAVPELRARVTALLRRPSRLRGNHLSVANIVVDR
                     DSLEAVVDGDSLPLALKQFQLLELLVRRKNHITPKRMIEEALYGFADEVSTNSIEAQI
                     YKLRLALRAAGAEASIETRRGIGYRLVELKPARTDG"
     gene            14163..14531
                     /locus_tag="VGM46_07515"
     CDS             14163..14531
                     /locus_tag="VGM46_07515"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEY4192479.1"
                     /translation="MAERKAIAPADLALLDPDGVFAGRLAGDRAALIAWLDAMQTDGA
                     REEQLKHIATLAHRLAGAAGTFGHVEVGDVALKLEALILDREAGRGVSVRRDAVRLAA
                     AALILALDESLASSNGGVCL"
     gene            14740..16086
                     /locus_tag="VGM46_07520"
     CDS             14740..16086
                     /locus_tag="VGM46_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding protein"
                     /protein_id="HEY4192480.1"
                     /translation="MSDSIKRPAIWTRRQTLLTGAGMAGAAMLPMGFTRAAFGQDKPA
                     IGTWPAGSEGSSVFVGASVPLTGTYAVQGEDELKGYQLAVEHMNSGHELMKAISPKTT
                     KGVLGKEVKLGVADSAAKPNEAVQAQQRFISENKAVMITGGTSSAVAVALNKLAQREK
                     VLFVCGISGSNDTTGKDCVRYGVRQNFFGQTTASAIAPVITKEFGKDLKVAYMTPDYT
                     YGHTVTKSMQDMTASAGWTTVTNQVSPLGAPDFSSYLLNIANSGADVLVNVNWGHDAV
                     LSIQQAKQFGVLDKMKLVVPYQVPFLARETGGLMQGVYAATEYWWTMADQFPLAKMFN
                     DTFEAKFGYKPEWGAENSYISFAHWARMVEEAGSFYPPDVIKAYEKGETIPSLTGDVH
                     YRPEDHQCVRPVVVVKGKMEADMKNKEDYYDIVEIVPGEGLMQKPDAFGCKLGDYT"
     gene            16189..17064
                     /locus_tag="VGM46_07525"
     CDS             16189..17064
                     /locus_tag="VGM46_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY4192481.1"
                     /translation="MINWANFVSQLFNGLALGALLALISSGLTIIYGTLGVLNLAHGA
                     MFMLGGYAGYVAYTYTDSFVVAVIAGSLFVMIVGILMERLVIRHFYSRPNEDQLLVTF
                     GIGICFVEIVRFFFSSDSKLVPPPPLFQGITSLGFMLYPTYRLAVVGIVAVALIALFL
                     VLYRSRLGMIVRAGIEDSVMVSSLGINVHRVFMIVFGIGAMAAGFAGIINAPVVSLTP
                     GIGDDILVETFVVVVIGGVGSFPGAVLGGLIAGEIISITSMFNPGYAYVMLFAAMTLV
                     LVLRPHGLLGAQGRE"
     gene            17078..18937
                     /locus_tag="VGM46_07530"
     CDS             17078..18937
                     /locus_tag="VGM46_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HEY4192482.1"
                     /translation="MLKSRPFLIEALTAIGLLAAPFILPYFGFAPNTVNRILVWGLFG
                     IGFDILFGFTGLLSFGQSAFYGTGGFVAAYLLTRAGFPYVIPALVIGMIVAAIVGYLV
                     GLIALRRTGIYFAMITVAIAEVFFFVEFNPLADWTGGENGLPGVPTPSLPLGFTTLHF
                     TTGWSLYQFLAVCYFIGIVLALRIVRSPVGAIFSAIRENPLRATAVGHNIHGYKLTAF
                     VIAAAYAGFAGGCLGVLQAFMPPDAFTFETSGQLVMQAAIGGTGTLFGPLLGAAVWLF
                     LQDFLQAALGLGAAWKLVLGLVFVLLVCFLRRGLIGGIEDLYALATGKGRPVEPVEDM
                     PEPVVQPMADMKPARLPAAAAPARRRAENNSTGPILQATGLTRRFGGLVANDDIDFSV
                     NHGALHGIIGPNGAGKSTFFKMLTCELPPSSGKIVFEGRDITGMSVTDVCQLGLTKSY
                     QVNQLFNRLTVRENVTVAALAELRGGFRLDLFRNMKKMPALTEQVERTLELVNLTGRV
                     DTPVSDLAYGEKRRLEIGLALASSPSLLLLDEPLAGMSPRERVETVALLKSISEGRTM
                     VVIDHDMDSLFELVSKVTVLQEGRVLVEGTPDEIKSNEKVQEAYLGGMHGVFE"
     gene            18934..>19558
                     /locus_tag="VGM46_07535"
     CDS             18934..>19558
                     /locus_tag="VGM46_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4192483.1"
                     /translation="MSLLEVAGLNSYYGDSHILFDVSMRVERNEVVALLGRNGAGKST
                     TLKSLMGVVTPKSGSVRFDGAEVAGKKSHAIALAGMQLVHEERRVFGSLNVEENLVLA
                     GLSAKGKWPLERIYEMFPRLRERRASRGTDLSGGEQQMLAIARALIRDPKVVLLDEPF
                     EGLAPIIVRDLLAVCKTLAAAGQTIVIVEQNIAAALSIASRVYILNNG"
BASE COUNT         3430 a         6274 c         6326 g         3528 t
ORIGIN      
        1 tggtcgcggc ggattgcggc aggatcgtcg ttgtcgacaa tatggtgcgt ggccgaattg
       61 ccaatctggt ttcggctctt ccatccgggc gcgtcgaaat cgtcaatggc gacatacgcg
      121 accagcggct gatgcagacg ctggtggccg gctgcgacac cgtattccat caggcggcgc
      181 tgcgcatcac ccactgcgcc gctgagccgg acgaagcgat gcaggtgatg gtcgacgcca
      241 cctatcgcct gctgcgccaa tgcgtggagg cgaaggttcg caaagtcgtc atggcctcgt
      301 cggcgtcgat ctacggcatg gccgaggatt tcccgacgac cgaaagccac catccctatg
      361 gcaaccgcac gctctatggc gcggccaagt cgttcgggga agggctgctg cgctccttca
      421 acgacatgta cggcctcgat tatgtcgcgc tgcgctactt caacgtctat ggcccgcgca
      481 tggacattca cggccgctat accgaggtga tggtgcgttg gatggagcgg atcgaggcgg
      541 gcgagccgcc gctgatcttc ggcgacggct tgcagaccat ggacctgatc cacgtaaccg
      601 acgtggcgcg cgccaacatt cttgccgcga ccgcgcctgc ttccgatacg gtctttaatg
      661 tcggctccgg cgccgaaacc tcgctgctcg gccttgcccg ccatttcgcc accgccatgg
      721 ggcgtccgga gcttcagccc gagcacaggg aagagcgcgc catcaacccg gtgccgcgcc
      781 ggttgtcttc caccgcggcc gcacgggaaa tgcttggttt ccgctcaaag gtcgagccgc
      841 aggaaggcat cgccgatctc gtcgcctggt ggcgcgacga aatgaagctt gcccggacaa
      901 cggagatggc atcgtgattc cgattgcaaa accgcttgtc gggcaggaag aggcagacgc
      961 cgccagccgg gtcatcctgt ctggctggct gacgcagggg ccgcaggtgg cggccttcga
     1021 aagcgagttc gccgccctgg tcggcgccga ccacgcctgc gctgtggcca attgcacggt
     1081 cgccctgcat ctggcgctga tggcgcttgg cgtcggcccc ggcgacgagg tcgtcaccgt
     1141 cagccacacc ttcatagcct cggccaacgc cattcgccaa tgcggcgcga cgccggtgtt
     1201 cgtcgatatc gagcaggacg gtttcaacat cgatccggcc ctcatcggca aggcgataac
     1261 cccgcgcacc agggcgatcc tgtgcgtgca tcagatcggc atgccctgcg acatgcaggc
     1321 catcatgcgc atcgcccgcg cccatggcgt cccggtcatc gaggatgccg cctgcgccat
     1381 cggttcgcag atacgcgtcg acggtcaatg gcaagctgtc gggcgtccgc tgggcgacat
     1441 cgtttgtttc tccttccatc cgcgcaaggt gctgaccgtc ggcgacggcg gcatgctgac
     1501 gacgcagaac cctgagtggg atgcgcggtt ccggctctgg cgccagcatg gcatgtcggt
     1561 ttccgacgcg gtgcgccacg gcagccgcga ggtgatcctc gaatcctatc cggtgcaggg
     1621 cttcaattac cgcatgaccg atatccaggc cgcagtcggg cgcgaacagc tgaagcggtt
     1681 gccgggcatc atcgccagcc gccgcaaacg cgccgagatt tacagcgccg caatcgccgg
     1741 catcgatggc ctcaccgccc cgcacgagcc ggattgggcg ctgtccaact ggcaaagcta
     1801 ttgcgttcgc ctagtgcccg gcctcgacca gcgcgcgatc atgcaggatt tgctggacca
     1861 cggcgtatcg acgcggcgcg gcatcatgtg catccacatg gaaaaggcct atgccgacct
     1921 gccgctgccg cattcgctgc gccactcgga aaaggcgcgt gaccattcca tcatcctgcc
     1981 gctctacgcg cagatgtcgc atgacgaaca gatgcgggtg atcgaactgc tggcggccgc
     2041 cgttgcccgg accagccgga cgccgcacat ggccgttgcc tgaggcccgg cgcgacatgc
     2101 cgggacctgc ctcacagccg cgcggttcgc tcaatgtgat gctgcaaagc atcgagcggc
     2161 gcatcggcat cctgcatctc gacctcaccg accttgaagt ggtgaccgag gccgcgaccg
     2221 gcgcttatgc ttctaccgcc gtcatcgccg ccctcgcggg cgccaaggtc cgcgcctgcg
     2281 cgcgcgacac ccataaccat ggcagcgccg aagacgccgt cgccgccacc cgtgaactgg
     2341 cgcggctggc gggcgtcgag gaccagatca tcttcttcca cggcgtgccg cccgaggtgc
     2401 tgcgcagctg cgacatcctc accaacaccg gccgcatccg gccgatcacc cgcaacatgg
     2461 tggacctgtt gcaggaagac gccgtcatcg cgctgatgtt cgaggcgtgg gaatttcgcg
     2521 gctccgacct cgatctcgcc gcctgccgcg agcgccgcat ccgcatcgcg gcggtcaacg
     2581 aacgtcacgg caatgtcggc gtgtttccgt tccttgggcc tttatgcgtg tgcctgctgc
     2641 gcgatgccgg catgaatgtc gccggcaagc gcatcgcggt catctgcgac aatcccttcg
     2701 ccacctttct gctggccggc ctggccgaag tcggcgccga ggccgcggcc ttcgaaagcc
     2761 ctttgcaatt gacgccgggg ccgtgggatg ccgtcgtgct ggcgctgctg ccgcaagacg
     2821 atccgcttgg ccagcgccat ctggaagtca tcgccgaacg cgcccccggc gcactcctcg
     2881 cccagttctg gggcgatgtc gaccgcgccg cagtcaagga gcttggcctc cgcatctgcc
     2941 cagaaatcga acccggacgc ggccacatgg gcatcctgct caaccgccta ggccacgaac
     3001 ccatcgtgcg cctgcaagcc ggcagcctga aagccgccga aatcgtcctg cgcggcggcc
     3061 cgttcatgcc cggcgggata gcttcgctgc tgtagaggca ataggcgtgg tttctctatt
     3121 ccgtcatgtc cttcatcaat ttatctaata tgcatgtcca tacttagaac gggttccgac
     3181 ggcgaccagg aaaggatttc ttgtgttcga cgtgttgttg tcttcgagca ttgttgacgt
     3241 ttttgcgtcg tttcgatggg gttttaatca ctacaggttt ctttttgggg gttaccttgg
     3301 ttggtccgct acttttcttt gtaacgccgt tttctggctc cttgtttgct gatgccgcta
     3361 gcaactcaac tgcgtgaacc tgccgttcga gaagttgtag cgcatcgctt tcgaactcgt
     3421 cagatgatcg gttcccctca tactgtaaat acagagatat cagggctatc aggagggtta
     3481 agatgggcag tcccaagacc atgaagcgcc ccatgatgcg acccaggaca ggggcaactt
     3541 ccgccgcttg cttaacagca tcttcgggcg ttatctcgtg agccttcgct cggcgtgcga
     3601 tttcttcgag tttgtgtaag attgcccgcg agatgggagg cccggaaacc atttccagtt
     3661 ccccacccct agtattgaat acgccatcta ccagcttcgc catccgtccg cagcgaggac
     3721 aagtcatccg gctgccagac aaggtgatat tagatgaatt ctcaatgtga attccgccgt
     3781 ggccatcaaa tatgcagcca cagtgccggc aataccctgg aatgcttagc atacgtcaaa
     3841 acctccatcc gcacggttca gtgtatctga gtcgaatgag ttctttaaaa taacaattac
     3901 ccaaacaggt tcaaaccatg aacaaaatct ggcatgatcg cctcatgcct attgtttcac
     3961 cgattctcca gaacccgctg atcgacggtc gccagtcgga gcgcgccatg ttggtgcggc
     4021 ggggggtgca gcggctgctc atgcagatgg gcgcgcatgt gctgccggaa ctgtcgctcg
     4081 ccaccggccg tcgcgccgat ctcgttgcgc tgacgcgctc gggcgatatc tggatcatcg
     4141 agataaaatc ctcggtcgag gactttcgcg tcgaccgcaa atggccggat taccggctgc
     4201 attcggatcg cttcttcttc gccacccatc ccggcgtgcc ggcggagatt tttcccgaag
     4261 aatgcggctt catcctgtcg gacggctatg gcgccgaaat catgcgcgac gcacccgagc
     4321 accgcatggc cgccgccacc cgcaaggcgc tgacgctgcg catcgcgcgc gccggcgcgg
     4381 cgcgcctgct ggcagcggaa ctggccggcg tttccgtgcc ggccatcgag ggcgagagcg
     4441 aataacggga gaaagtcggc agaaagcagt gaatatgtga gcagcgaggc gacaggtaga
     4501 aaacactacc ccctactccc ctactcccct actctcaccg cggcgcgcgc ttggccagaa
     4561 tccgttgcag cgtgcggcga tgcatgttga ggcggcgcgc cgtttcggaa acgttgcgct
     4621 cgcacatctc gtagacgcgc tggatatgtt cccagcgcac ccggtcggcc gacatcgggt
     4681 tttcgggcgg cgcggcgcgc tcgccggaag tgcgggtgag ggccgcgaac acatcgtcgg
     4741 catccgccgg cttcgacaga tagtcgattg cgccaagctt caccgctgtc acggcggttg
     4801 cgatattgcc gtagccggtg aggatgacgg tgcgcgcatc cgcccgcttt tcgcggatgg
     4861 cggccaccac gtccagcccg ttgccgtcgc caagccgcat gtcgacgacc gcataggcgg
     4921 gcggattggc gcgcgccttg ccaaccgctt cttcgacgct ttccgccgtt tcgacggtaa
     4981 aaccgcgcgt ttccatggcg cgcgccagac gggtgaggaa gggcttgtcg tcctccacga
     5041 tcaagaggga ggtgtcgtcg cccgttacca tgccgtcagc ggcatcttcg ccgttcattg
     5101 ccgtataatc cttgtttgcc agctttccat gcagatatag ttcttgagcg ctcttgtcca
     5161 attttcgcca tccgcccgcc atttttgcga gttcaggcgg tgtcgaacat gtccgtttcg
     5221 gcaggatggc ggttgacgaa attggcgcgc ggccagttga tttccacgat ggcgccttcg
     5281 cccggcccgt tggagttgct gaaggcgatg ctggcgcccg agcgttcgag cagcgtcttg
     5341 gcgatgaata gcccaagtcc caggccgccg cccggttccg cgccctggcg ggtcgacata
     5401 tagggttcgc cgatccggtc gatgatttcg gcgggaaagc ccggcccatc atcggcaatc
     5461 gaaaaattga cgtggttgtc atcccagttc cagcggatga tgaccttcgc ctgcgcgaag
     5521 tcgacggcgt tctcgaccag attgccgagg ccgtagagaa cgccggggtt gcgccggcct
     5581 accggctccg ggccgattcg cgcgccaggt tcaagcgcga tggaaatgcc gaaatcacgg
     5641 tgcggcgcga tcacctcttc caccagcgag gtcagcggca attgcgaaag ctgcggctcg
     5701 ccttcggagg aaaggctggt cagtcgcttg aggatctcgc ggcaacgttc gctctgcgag
     5761 cgaagcaggg ttacgtcctc gcggtatttc ggatcggaac cgagcgcctt ttccatttcg
     5821 cgcgccacca gcgtgatggt cgccagcggc gtgccgagtt cgtgcgccgc cgccgccgcc
     5881 agcccgtcca gcgccgacag atgctgctcg cgctgcaaca ccagttcggt ggcggcaagc
     5941 gcgttggcca gaagccgcgc ctcctcggcg acgcggaaag cgtagatcgc ggtaaacgag
     6001 atcgacgaaa acactgccac ccatgtgccg gcgacataga cgaagggcag ggtcagcggc
     6061 tcgcccgctt gccatggcag cggcaggtgg aagaatacca ggagcgtcgc cagcgccatc
     6121 accatcccgc caagcgcggc ggtgaggcgc agcggcagcg aggcggccga aatgaccacc
     6181 ggcaccgtca tcagcagcga gaacgggttg gtcaggccgc cggtcatgta gagcaggccg
     6241 gccagttgca ggctgtcgaa caccagaatg cccatcgccg ccggcggttt cagccggtgc
     6301 gccgccggat atcgaaaggc gaggataaga ttgagccatg ccgaacaggc gatcagcgcg
     6361 aagcagacgc cgaccggcag cgggaattgc agcccgtagg cgaccacgat gacggtgatg
     6421 ctctggccga caatggcgag ccagcgcagg cggatcagcg tattgaggcg cagcctctgg
     6481 ctttgccgga aatcgggtgc gcggtgaaaa tcgatcattg gccggctttt atagcgatgc
     6541 ggggcataat tccagcgtgg cggttggttc gggttccggc gtaggggccg agacagttcc
     6601 gctttttacg tttatgcgat ttcgaattga aacacgctag cttccacgcg gcttggcgcg
     6661 ggcggtggcg gtggcggcga gcgggttttc cggccagaca tgcttggggt agcgcccgcg
     6721 catgtcggcg cgcacatcgg cccacgaacc gcgccagaag ccgggcaggt cgcgcgtcgt
     6781 ctggatcggc cggtgcgccg gcgaaagaag ttcgagcgtc agcggtaccg ttccggcggc
     6841 aatcgatggg tggcggtcga agccgaacag ttcctgcacg cggatcgcca gaaccgggcc
     6901 ttcctcgtcg taacggatag gcacccggct tcccgaaggc gcatcgaaat gcgtcggcgc
     6961 cagggcctcg accttgcgtt gcaactcatg cggcaccagc gaggcaagcc cggccgaaag
     7021 cgcggctgcg tcgatcgcgg aaaagcttgc cgcgccggtc aggaaaggtt cgagccaggt
     7081 ttcgagattt tcgatcaggg cagcatccga aacgtccggc catggcgctc caaggccgcg
     7141 atgcaggaag gaaagccggc ggcgaagcgt ttccgcctcc ttgctccatg gcagcagcga
     7201 cagcccatgt tcgcgcaccg cttcgataat ggcgcggtcg gcatccgcgc ccgagggcgc
     7261 cggcaacatg cgctccgcca gggtgatcgc gccgagccgc aaggtttcgc gcacccgcac
     7321 cgcgcgcttt tcccggtcga acagcgtttc gcgccgtgcc tcgatccggt cacccagggc
     7381 ggcgcggata tcggcttcat cgacggccgc cgccgccgtg atgcgggcgt tctgcgcctt
     7441 gccggtcagg tcggcgacga cgagaaaagc ttggccagcc agcgggtcgg cggcatcgag
     7501 cgccgcgccc gagccattgg ccagcacgaa acgtccgcgt tcgccgcgcg ccttggcaac
     7561 gcggtccggc cacgcatgca gcaacaaggc tccggctttg ccctcttctc cgttgcggga
     7621 agggcttgcg cgcggcaccg gggcgagggt gcttttcgcg tcttgccggt ctattcggcc
     7681 gccgtgtttt cccgagccgg atgccaaggc attgcgcgcc agccgctgcg ccaattgcct
     7741 tgcggatgcg gcgcggggcg agtgctcgcc gtgaaagcgc atcagcctgc gctcaagatc
     7801 gacgccgtcg ccgcccagcc cgcgttcggt gagcagcacg gcaagttcgg cggcggcctg
     7861 agcggatggc gcgtcgccgt ccttcgccgc ttccgccacc atgtgcgcca gccgcaccgg
     7921 cagcgccagc ccgcgcatgg cttcgccctg tttggtcaat ctcccggcat cgtctatcgc
     7981 gccaagcgcc tttagcaagg cgcgggcttc ggcaagcgcc ggtgccggcg gcgggtcgag
     8041 gaaggacagt gtcgtggggt cggcgacacc gaaggcggcg cagtcgagca ccagcccgga
     8101 aaggtcggcc tccagtattt ccggcggcgt gaacgccggc agcgccgccg tctgctccgc
     8161 ccgccacagg cggatcgcca cgccgggctg cgtacgcccg gcgcgcccgg cgcgctggtc
     8221 ggcggaagct tttgccgcgc gcaccgtttc cagccgggtc aggccgcttg ccggctcgaa
     8281 acgcggcagc cgcgccagcc ccgaatcgat gacgacgcgc acgccgtcga tggtgatcga
     8341 ggtttcggca atggaagtgg cgagcaccac tttgcgccgg ccggcgggcg acggcttgat
     8401 cgccgcgtcc tgcgccttgc cgtcaagcag gccgtagagc ggcacgatat cggtgtcgct
     8461 gccgatccgc ccttccagcc gctcggcggt gcgcaggatt tcgcgctggc cgggcaggaa
     8521 agcgaggatg ctgccggttt cgttggcaag ggcgtcccgg atggccttcg ccatggcgtc
     8581 ttcgacgggc gtgcccgccg ggcgctcctc ataacggata tcgacgggaa aggcgcggcc
     8641 ttggctttcg atcacaggcg catcgccaag caggctcgcc acgcgcgcgc cgtctagcgt
     8701 cgccgacatc accagcaggc gaaggtccgg ccgcagggcg ctttgcacgt caagcgccag
     8761 cgccagcccg aaatcgccgt caagcgagtg ctcgtgaaac tcatcgaaca gcacggccgc
     8821 gatgccgggc aattcgggat cgtcaagcag catccgcgca aatacgccct ctgtcactac
     8881 aaggattctg gtgcacgccg aggtgcgatt ttccatgcgc atggcgtagc cgaccgtctc
     8941 acccggttcc tcgccaagca gggccgccat gcggcgcgcg gcggcgcggg cggcaagccg
     9001 gcgcggctcc agcagcacaa gcttgccatc gccgcgccag ggcgccgaga gcagcgccag
     9061 cggtgccagc gtggtcttgc cagcgccagg cggggcgacc agcacggcgc aattgccagc
     9121 ggcaagcgcc tcgccaagcg ccggcaacac cgccgatatc ggaaggtctg gcaaatcctt
     9181 cttcgtcacg tcgcctcgtt gtcgttggcc gcaccggcgg ctgaagaact gcgcatatca
     9241 gtggcggccg gcgctgtaca accggcgtct gccaagacgc tcagaagcgc cggctgacga
     9301 agggcgaacc ggccagcccg aagcgccaag gccagtcgac ggcgcggcta agcccgatgc
     9361 gcgtgccggt ggcgacgggc atctcttcgc ccgccagttt cagcgaaaag ggcggcttgt
     9421 ccagcgcaag cccgttcatg gaaatatcga cgcccagcgc ctggcacagc cgccccggcc
     9481 cggagcacaa cagccgcggg ttttcggtgt tgcggcgcgg ggccatggcg tcgaggccag
     9541 cggtcggctc cagcgcgcgg atgagtgctg cgctgcccgg caggcagacg aaattaaggc
     9601 accagtggat gccataggaa cgatagacat aggcgcaggc cggcggcccg aacatcgccc
     9661 ggtttcggtt ggttgggccg cgataggcat gcgatgcggg atcgtccggc tggtaggcct
     9721 cggtctcgac gatcacgccg ccaacgccgt cgaccaggat cgtcgcgccg atgaggtcct
     9781 ttgcaacggc aagggcgggc ctggagaaaa acgcggtcag gttcatggtc gagcctttga
     9841 gatcagcaac cgttcagcca aggcgtcgct atccttcagg cggcatcgtt gcgcaagccg
     9901 gagcatggct aaagtttcag gatgcgcctg ccaataggct ttcgcccggc atggagacgg
     9961 tggcgggcga aagccggatg cgaaaggccg ccccgccatc ctcggcgtcc tcgacccaga
    10021 tgcgcccgcc atgctgggcg atgatgcgct gtgagatggc gaggccaagt cccgcgccgt
    10081 gcggcttgcc gctcatcgtg tcgcccagtt gccggaaacg ctcgaagacg atctcgcggt
    10141 cgtcggcgtg gatgccgggg ccgttgtcgc tgaccgaaat ttcgaattcg tcgccggagc
    10201 gcgcgaggtg cagtgtcgcc cgcccgccgc cctgctgtga aaacttcgcg gcattgccga
    10261 gcaggttgat caccacctgc acaagccggt catggtcgcc catcaccagt gccgggtcgg
    10321 cctctatgcg cagcgccagc tcgatccggc cggcggcgaa aacctgatgc atggcggcgg
    10381 tggattcgcg gatgaccgcg gtaaggtcga ccacggcgct ttgcgctgga aaagcaccgg
    10441 tcgcctcgat cttggaaagg tcgagcaact ggttgatgag acgggtcagg cgctcggatt
    10501 cggtgacgac gatgcgcagg aactcgcgcc tttcctcgcc cggcaaatcc ggatgatcgc
    10561 gcaggatttc gcagaatgcc cggatcgagg cgagcggcgt gcgcaattcg tggttcacgg
    10621 tggcgacgaa atcgtccttc agccggtcga gtacctgaag ctgggcattg gcctcggtca
    10681 gctcggtggt cgccgcctcc aatgcgcggg acttgtcctc aagctgccgg ttggcctcga
    10741 tgatgcgcga cgtctcgtcc agaatgcgca tcatctcgtc gacgcccagt tgctcttcct
    10801 cgacgatggc gctgaccaga acccgtgcgg aagccgcgcc gatgacgccg gccagcaagc
    10861 gttcgtaaaa cagcacggtc tcggcgtcgg cctgcccggt ggcgtcgggg tccagtccgc
    10921 gcatatgcgc ccagttggca aaggcggcgg cggctttttc gcggccaaga aagcgcgaaa
    10981 ccagcgacat gaggtcggga acgcgcgccg tgcgccgcca tatctgcgct acttcgtcga
    11041 ggtgctcggt atccacgaat aaggcggcgc gcgcctgttc gaccgggctt tggtggccca
    11101 tcagcgagac gatcagaaac gcgccgacat tgaccagcag cgaccagaag gcgctgtggg
    11161 tcaccgggtc gaggccggtc agcccgaaca gcgcataagg tttcaaaagc gcggccccga
    11221 acggcccgtc ggcgacgaaa ccgatttcca gcgcgccgga acgggcgaag gagggcagta
    11281 gcagcgtgta gaaccacacc agcacgccgg ccgagatacc ggcaaatgcg ccggccttgg
    11341 tggcgccgcg ccagaacagg ccgccgaaaa tggccggcgc gaactgcgcg accgccacga
    11401 acgacatcag cccgatcgac accagcgcga actgggcgct gtcgtggcgc atgtagaggt
    11461 agccgagcgc catgacggcg atcatgacga tgcggcgcac ccacagcaga agccgcgtca
    11521 ggtctggctt tttcgccagt cccagcgcct tcacccgcag cagcgccggc atcaccacgt
    11581 cgttgcacac catcaggctg agcgccgtcg cctccaccac caccatgctg gccgaagccg
    11641 aaagcccgcc aaggaaggcg atcagcgcca gaaccgggtg cccggtcgcc agcggcagcg
    11701 acaaaaccag attgtcggga tcggtatcga cgggcagcag catccgcccg gcgatggcga
    11761 tcggcaggat gaacaggttg atggccagca gatagaacgg gaacagccac agcgcgcggt
    11821 tgaggtggcg ctcgtcgaca ttctccacca ccatcacctg gaactggcgc ggcaggcaaa
    11881 acaccgacat catcgccagc agcgtcatgg cgatccagtc atagccgtaa cggtgcgggg
    11941 tatattcgaa gatggaatga atctcgggct gggccatcgc ctgggcgaaa agatcgccga
    12001 agccatggaa cagtccgtag ccgacgaaca gccctatcgc ggtcaggcaa acgaatttga
    12061 ggatggattc aagcgcgacg gccaccacca tgccggggtg gtgctcgccc ggatggatgc
    12121 tgcggctgcc gaacaacata gcgaagatgg tcaggaccac tgccgcccac agcgcgccgt
    12181 cggccagcag cggctgcacc atcgggtggg cggcctcgcc gctttggcta agggcggcga
    12241 acccgctgga gatggatttg agttgcaggg cgatatacgg caccagcccg accaccgcga
    12301 tcacggtgac gagcgcggcc agcccgccgc tgttgaagcg cgcgccgatg aggtcggcga
    12361 tggaggtgat gcggttggcc tggcttaccc gcaaaatctt tcggatcagc accgggccga
    12421 gcaggaatgc cagtgtcggg ccaagataga tcggcaggaa atcgaagccc gactgcgagg
    12481 ctcgtccaac cccgccattg aacgtccagg aactgcaata aaccgccagc gacagcgtgt
    12541 agacgacgct ggagccggtc agccgcgaaa tgcggccgct gctgtgctcg accagatagg
    12601 cgacggcgaa cagcagcgcc agataggcga cggcggcggc gatgatgaac tgctggctca
    12661 tggccgcttg gtgtcccggt cgcggccggc tgccggattg ccgccgcgtt ccacgatcag
    12721 cgcggttgcc gcgataagcg ccgcccatac cccgaacaga tagatgtaga gcgccggcca
    12781 gccgaacagc gtgccgctga ccagattgag gatgggcggg ttgaacagca cgaaagcggc
    12841 gatgaacagt ccgaccagcc ggtcccgttt tgtcgtgcgg cgaagcatcc cgcgccgcct
    12901 atgaatcgga aatggcggtg gaaagctgcg gtttttcggc gagcacgctc gccacgcagg
    12961 tcaccacgtc gccgattgcg aacggcttgc tgatataggc gtcggcgccc agagccagcc
    13021 cggcccgccg gtccgcctcg cgccctttcg ccgtcagcat gatgatgcgg acgccatcat
    13081 aggcggggct ggcgcgaagc atccggcaga cgtcgtagcc gttggccttc ggcatcatca
    13141 gatcgagcag gacaaggtcg ggaaccgcct gcgcgaccgc atccagagcc tcctggccat
    13201 cggtggcggt gcgcacgtca tggccctccc gccgcatcag ataggtcagg gcgagcagga
    13261 tgttgggatc gtcatcggcg atcaggattg aggccgacat gcaccgtttc cctccgtatt
    13321 ggcttgcaaa accagtaaac ctcttgtgtt tggtcgacaa gagcagcaaa aatacccgct
    13381 tctgcatccg gggcgcagcg atatgatgaa tggtgccggc agactatcca gcggtacggt
    13441 ttccgacggg agcgacgggt tgcccggcat ttcgcagccg cgcatcctcg tggtcgacga
    13501 tgaccgcgat attgcgggcc tgttgcgccg cgagctggtt tcgctgggtt tcaccgtgga
    13561 ttccgtcgag tttgcgggcg atgcgctgct cgccgcgcgc gatgtcgcct atgccttgat
    13621 gattgtcgat ctcggcctgc cggacaggga cgggctggac ctcgtgcggg acatgcgcag
    13681 ccggctgatc gaggcgccga tcctggtgct gactgcccgc aacggggtgg aagaccgcgt
    13741 caccggcctg tcctgcgggg cggacgacta tctggtcaaa ccgttcgcgg ttcccgaatt
    13801 gcgggcgcgg gtgaccgcgc tgctgcgccg tccgtcgcgg ctgcggggca accacctgtc
    13861 ggtcgccaat atcgtggtcg accgcgattc gcttgaggcc gtcgtcgatg gcgacagcct
    13921 gccgctggcg ctcaagcagt tccaactgct cgaacttctg gttcgccgca agaaccacat
    13981 caccccgaag cggatgatcg aggaggcgct ctacggcttt gccgacgaag tctcgaccaa
    14041 ctcgatcgag gcgcagattt acaagctgcg cctggcgttg cgcgccgccg gcgccgaggc
    14101 ctccatcgag accaggcgcg gcatcggcta tcggctggtg gaactgaagc cggcccgcac
    14161 cgatggctga gaggaaggcc atagcgccgg ccgatctcgc attgctcgat ccagacggcg
    14221 tctttgccgg acggctggcc ggcgaccgcg cggccttgat agcctggctg gacgccatgc
    14281 agacagacgg cgcgcgcgag gagcagttga agcacatcgc cacgcttgcg caccgactgg
    14341 cgggggcggc cggcacgttc ggccatgtcg aagtaggcga cgtggcgctg aagctcgaag
    14401 cgttgatcct ggaccgcgag gccggccggg gggtgtcggt ccggcgcgat gcggttcgcc
    14461 tggcagcggc cgcgctcatc ctggcgctgg acgaaagcct ggccagctcc aatggcggcg
    14521 tttgtttata aaagtcagtt tcaggtcagg ttgaattcag gaagcggtca gcttgatggc
    14581 ttttactgac ttgtccggcg cgggtggagg agccgatccg gatggtacga cgccaagtgc
    14641 gcctacgggc gtggcttgtc tgcggtcgtg ggaggtctcc tctgcgtgaa cgaattcgtg
    14701 tgccgccagg cgcaacggaa atgcaaggga ggaacttgaa tgtctgattc cataaaacga
    14761 ccagcgatct ggacccgccg ccagaccctg ttgaccggcg cgggaatggc cggcgcggct
    14821 atgctgccga tgggcttcac gcgggcggcc tttggccagg acaagccggc catcggcacc
    14881 tggccggctg gttcggaagg ttcctcggtt tttgtcggcg cttccgtgcc gcttaccggc
    14941 acctatgcgg tgcagggcga ggatgagctg aaaggctatc agcttgccgt cgagcacatg
    15001 aacagcggcc atgagctgat gaaagcgatc tcgcccaaga ccaccaaggg cgtgcttggc
    15061 aaggaagtga agctcggcgt cgccgattcg gccgccaagc ccaatgaagc ggtgcaggcg
    15121 cagcagcgct tcatcagcga gaacaaggcg gtgatgatca ccggcggcac ctccagcgcc
    15181 gtcgcggtcg cgctgaacaa gctggcgcag cgcgaaaagg ttcttttcgt ctgcggcatt
    15241 tccggctcga acgacaccac cggcaaggat tgcgtgcgct acggcgtgcg ccagaatttc
    15301 tttggccaga ccaccgcctc ggcgatcgca ccggtcatca ccaaggaatt cggcaaggac
    15361 ctgaaggtgg cgtatatgac gccggactac acctatggcc acaccgtcac caagtcgatg
    15421 caggacatga ccgcctccgc cggctggacg accgtgacca accaggtttc gccgctcggc
    15481 gcgccggact tctcgtctta cctgctcaac atcgccaatt cgggcgcgga tgtgctggtc
    15541 aacgtcaact ggggccatga cgcggtgctg tcgatccagc aggccaagca gttcggcgtg
    15601 ctcgacaaga tgaagctggt ggtgccttac caggtgccgt tcctggcgcg cgaaacgggc
    15661 ggcctgatgc agggcgtcta tgcggccacc gaatattggt ggacgatggc cgaccagttc
    15721 cccctggcca agatgttcaa cgacactttc gaggccaagt tcggctacaa gccggaatgg
    15781 ggcgcggaaa attcctacat cagcttcgcc cattgggcgc gcatggtcga ggaagccggc
    15841 agcttctatc cgccggacgt catcaaggcc tatgaaaagg gcgaaaccat tccctcgctc
    15901 accggcgatg tccactaccg tcccgaagac catcagtgtg tccgccccgt cgtcgtcgtg
    15961 aagggcaaga tggaagcgga tatgaagaac aaggaagatt actacgacat cgtggaaatc
    16021 gttcccggcg aaggcttgat gcagaagccg gacgctttcg gctgcaagct tggcgattac
    16081 acctgacaag acaggatcga cgcggcgtgg gcggcatttg ccgtccgcgc cgcccgcccg
    16141 cgcgaatttc gccgggcttc ccaggggaag agggtccggt aacccgttat gatcaattgg
    16201 gctaatttcg tctcgcaact attcaacggg ctggcgctcg gcgccctgct ggcgcttatc
    16261 tcgtcgggcc tgacgatcat ctatggcacg ctgggcgtcc tcaacctcgc ccatggcgcg
    16321 atgttcatgc ttggcggcta tgccggctac gtcgcctaca cctatacgga ctccttcgtg
    16381 gtggcggtga tcgccggctc gctgttcgtg atgatcgtcg gcatcctgat ggaacgcctc
    16441 gtcatccgcc acttctacag ccgtcccaat gaggaccagc tgctggtcac cttcggcatc
    16501 ggcatctgct tcgtcgaaat cgtgcgcttc ttcttctcca gcgattccaa gctggtgccg
    16561 ccgccgccgc tttttcaggg catcacctcg ctcggcttca tgctgtaccc aacctatcgc
    16621 ctggccgtgg tcggcatcgt ggcggtggcg ctcatcgccc tgttcctggt gctctaccgc
    16681 agccgcctcg gcatgatcgt gcgcgccggc atcgaggatt ccgtcatggt ctcctcgctc
    16741 ggcatcaatg tccaccgcgt cttcatgatc gtgttcggca tcggcgccat ggcggcgggc
    16801 ttcgccggca tcatcaacgc gccggtggtt tcgctgacgc ccggcatcgg cgacgacatt
    16861 ctggtcgaga ctttcgtggt ggtggtgatc ggcggcgtcg gctcgtttcc gggcgcggtg
    16921 ctgggcgggc tgatcgccgg cgaaatcatc agcatcacct cgatgttcaa ccccggttac
    16981 gcctacgtca tgctgtttgc ggcgatgacg ctggtgctcg tgctgcgccc tcacgggttg
    17041 ctaggcgccc aaggcagaga atgagcgcgg tagcccaatg ttgaagtcac gtccctttct
    17101 gatcgaggcg cttaccgcaa tcggtttgct ggccgcgccc ttcatcctgc cttatttcgg
    17161 cttcgcgccg aatacggtca accgcatcct cgtctggggg ctgttcggca tcggcttcga
    17221 catcttgttc ggcttcaccg gcctgttgtc gttcggccag tcggccttct acggcacggg
    17281 cggcttcgtc gccgcctatc ttttgacgcg ggcgggcttt ccctatgtca ttccggctct
    17341 ggtcatcggc atgatcgtgg cggcaatcgt cggctatctg gtcggcttga tcgcattgcg
    17401 gcgaaccggc atctatttcg ccatgatcac ggtggccatc gccgaagtgt tcttcttcgt
    17461 cgagttcaat ccgctggccg actggaccgg cggcgaaaac ggcctgcccg gcgtgccgac
    17521 gccaagcctg ccgcttggct tcaccacctt gcatttcacc accggctggt cgctctacca
    17581 gttcctggcg gtgtgttatt tcatcggcat cgtgctggcg ttgcgcatcg tgcgctcacc
    17641 ggtcggcgcc atcttcagcg caatccggga aaacccgctg cgcgccacgg cagttggcca
    17701 caatatccac ggctacaagc tgacggcctt cgtcattgcc gccgcctatg ccggttttgc
    17761 cggcggttgc cttggcgtgc tccaggcctt catgccgccg gatgcgttca ccttcgaaac
    17821 ttccggccag ctcgtcatgc aggcggcgat cggcggcacc ggaaccttgt ttggaccgct
    17881 gctgggcgcg gcggtgtggc tgttcctgca agacttcctg caagcggccc ttggcctcgg
    17941 cgcggcatgg aaactggtgc ttggcctggt cttcgtgctt ctggtctgct tcctgcgccg
    18001 gggcttgatc ggcggcatcg aggatcttta cgcgcttgcc accggcaagg gccggcctgt
    18061 cgaaccggtg gaggacatgc cggagcctgt cgtccagcct atggccgaca tgaagccggc
    18121 gcgcctcccg gcagccgccg cgcctgcgcg ccgccgtgcc gaaaacaatt cgaccgggcc
    18181 tatcctccag gcgacgggcc tgacccggcg cttcggcggc cttgtcgcca acgacgatat
    18241 cgatttttcc gtcaatcatg gcgcgctgca cggcatcatc ggcccgaacg gcgccggcaa
    18301 gtcgaccttc ttcaagatgc tgacctgcga actgccgcct tcgtccggca agatcgtttt
    18361 cgaaggccgc gacatcaccg gcatgtcggt caccgatgtc tgccagctcg gcctgaccaa
    18421 gagctatcag gtcaaccagc tcttcaaccg gctgaccgtt cgcgaaaacg tcaccgtcgc
    18481 cgcgcttgcg gaattgcgcg gcgggttcag gctcgacctg ttccgcaaca tgaagaagat
    18541 gccggcgctc accgagcagg tcgaacgcac gcttgaactg gtcaacctga ccggccgcgt
    18601 cgacacgccg gtctccgacc ttgcttatgg cgaaaaacgg cggctcgaga tcggcctggc
    18661 gctggcttcg tcgcccagcc tgctgctgct cgacgagccg ctcgctggca tgagcccgcg
    18721 cgagcgggtg gaaacggttg ccttgttgaa gtcgatcagc gaaggccgca ccatggtcgt
    18781 catcgaccac gacatggact cgctgttcga actcgtctcg aaggtcaccg tgctgcagga
    18841 aggacgcgtg ctggtggaag gtacgccgga tgagatcaag agcaatgaaa aggtgcagga
    18901 agcctatctc ggcggcatgc acggggtgtt cgaatgagcc ttctggaggt cgcaggactc
    18961 aacagttatt atggcgacag ccatatcctg ttcgacgtgt cgatgcgcgt cgagcgcaac
    19021 gaggtggtgg cgctgctggg ccgcaatggc gccggcaagt ccacgacgct gaaaagcctg
    19081 atgggggtgg tgacgccgaa atccggttcg gtcaggttcg acggtgcgga ggttgccggc
    19141 aagaaaagcc acgccatcgc gcttgccggc atgcaactgg tgcatgaaga gcgccgcgta
    19201 ttcggcagcc tcaatgtcga ggaaaacctt gtgctcgccg gattgagcgc caagggcaaa
    19261 tggccgctgg aacggattta cgagatgttc ccgcgtctca gggaacgtcg ggcaagtcgc
    19321 ggtacggatc tgtcgggtgg cgagcagcag atgctggcca tcgcccgcgc cctgatccgc
    19381 gatccgaaag tggtgcttct ggacgaaccg ttcgagggac tggcgcccat catcgtccgc
    19441 gacctgctgg cggtgtgcaa gacgctggcg gcggcagggc aaaccatcgt catcgtcgag
    19501 cagaacatcg ccgctgccct ctcgatagcc agtcgcgtct acatcctcaa caacggcc
//
LOCUS       DASYCS010000235        10384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_203070,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000235 DASYCS010000000
VERSION     DASYCS010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 10384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10384
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203070"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VGM46_07540"
     CDS             <1..194
                     /locus_tag="VGM46_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006338618.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEY4192484.1"
                     /translation="PEGFEVTATSEDGTIMAFEHRHEAVAAVQFHPESIMSLGHDAGM
                     RMIENVVVHLPRKAREKAA"
     gene            206..1135
                     /locus_tag="VGM46_07545"
     CDS             206..1135
                     /locus_tag="VGM46_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEY4192485.1"
                     /translation="MTEIDTHNAARQRVATIAAWSIVIAFIVLALKTLAWFVTGSVAL
                     YSDALESIVNVIAACAALWAIRISHKPADTDHPFGHHKAEYFSAVLEGVLIVIAALLI
                     LTEVWRSWQHHPPLEQPWSGLAVNGAASLINAGWAFLLIRTGRAEKSPALVADGQHIM
                     TDVVTSIGVFAGLVGAILTGWQILDPLLAVLVAINILWQGWHVIGSSLNGLMDRAVDM
                     PEHMRIRDIISANSKGALEVHDLKTRIAGRATFIEFHMVVDADMTVSESHTICDRIED
                     ALKADIPSVRVTIHVEPDDEAKLPKGSVAVPFA"
     gene            1326..1625
                     /locus_tag="VGM46_07550"
     CDS             1326..1625
                     /locus_tag="VGM46_07550"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEY4192486.1"
                     /translation="MYGLIGKMRSAPGKREELLQILFEVTGQLPGCLSYIVARDPADT
                     TAIWITEVWKDAVSHKASLALPEIQAAIIKAKPFLAGFEFQIETEPAGGFGLPKS"
     gene            1966..3708
                     /gene="leuA"
                     /locus_tag="VGM46_07555"
     CDS             1966..3708
                     /gene="leuA"
                     /locus_tag="VGM46_07555"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEY4192487.1"
                     /translation="MSMTNETNAAGTTAKGMADAARKYAPYPTVGLFDRTWPSKVIDK
                     APIWCSVDLRDGNQALIDPMGHERKARMFQLLLDMGFREIEIGFPSASQTDFDFARWC
                     IEQGNVPADVSLQVLVQCRPELITRTFESLEGATRPIVHFYNSTSELQRRVVFEKDVR
                     GIKRIATDAAKMITDMAAKAAREPGHSGYRFQYSPESFTGTELEVALEICNAVTEIVQ
                     PTPDNKLIINLPSTVEMSTPNIYADRIEWMCRQLDNRENLVVSLHPHNDRGTGIATTE
                     LGLMAGADRVEGTLFGNGERTGNVDIVTLALNMYTQGIDPKLDCSDINRMKAVYEYSN
                     QLKIPERHPYVGELVYTAFSGSHQDAINKGMKALKKANTELWEVPYLPIDPADVGRSY
                     EAIIRINSQSGKGGIAYVLQADYGLNLPRNLQIEFSQDIQAITDAEGKEVPARRIHEQ
                     FLETYVDQPGARLKFVEHTTYPDTTTKGRRLVESTILDNGREVTITGTGTGPIDGFVE
                     ALSRHIGVPMSVLDYSEHSMQRGSNASAISYVEVEHPGGKLFGAGINTNISSASLEAV
                     VSAANRILGGWAPR"
     gene            3840..5594
                     /locus_tag="VGM46_07560"
     CDS             3840..5594
                     /locus_tag="VGM46_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006338644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192488.1"
                     /translation="MQHSTPAAPAVRETLERLLTSETFGRSERARELLRYLVERQQAG
                     EAERLKGFSIAVDVFGKDIDFDSSTDAVVRVQAGRLRELLNQYFATEGACDPVRISIP
                     RGSYIPTYELNAKPLLAAPNDLAESLVPGMPAAQSPAPDPGLNQKATPSIIWQLRCFW
                     AAMALIIVMLGVVIVRQAIFLAPDNEPAANGEGGISTSSIAAPSSLDVLPSVYIDMET
                     DGPQAGRVGASLRAGLSSFNTVDFIGRAAPRQPDPEADASSFVFHVLPDPSDGGITIE
                     LQNMVTGQVLLSRQLEKAEAEPSAIEDRIAGILTATLPTSGTIYNYIEQNGLQGGLTQ
                     CLLLDEKYYLDQNPVTHKPAYLCLEKLIAQGVKSPLAYSEIASLHMEAVTKHYSYPVG
                     ATVEQAIALARLGMRMGSTSASVHRSYGYINSRLGRTDAAIRLMRKAYELNTYDLTMA
                     AAYGYGLIFAGKYAQGTPILARAVEASSSHPTWWDFGLFAGEFMQGHSAEAKRASDAL
                     TTTEPKSHYLAARIISATASGDGEQAKQLIDKLGSEFPDFVANPRKVFLERNYPADLT
                     DQLVKALRQAGLPDASRS"
     gene            5578..6378
                     /locus_tag="VGM46_07565"
     CDS             5578..6378
                     /locus_tag="VGM46_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY4192489.1"
                     /translation="MPADRKAPPVLHSPVLHSLESGAGRQTIVFVHGFGATHNVWEKI
                     AAPLSQEARIIAYDLPGHGLSLDASGAGSAKSAARAILADLSERGIKAAHIVGHSMGG
                     AVATLMALAEPQRIVTLTLLAPGGFGPEIDGPLLRRYAKAADGEEIRACLAAMSGEGA
                     AISDDVLNLDLTMRTRPGQTAKLVEIAAAITRGDRQGVIAHEHLASLAMPVAVLWGTQ
                     DPVLPFLQTAAMPAHFTLHAIQAAGHMLITEAPEPVRQTIAFNLANSV"
     gene            6446..7063
                     /locus_tag="VGM46_07570"
     CDS             6446..7063
                     /locus_tag="VGM46_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192490.1"
                     /translation="MKDASETASPTGQTRSLSAALRDVKNALADRDDVVVDMRDAHRM
                     RLELLAVELAPVFKDVPVDVDSFDFVVSSGLQPRLWIDAVSHVAMGRDRRTYRFLRDT
                     RIGRVVLAESTDMKPVADQVARYVAERIVERQRMMDGGAEPASGGLIRAAWPEAEPPL
                     RQTGRNRFWQALFAGLALIVAGALFGLGISAVLFWGRISAALFSS"
     gene            complement(7053..7982)
                     /locus_tag="VGM46_07575"
     CDS             complement(7053..7982)
                     /locus_tag="VGM46_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY4192491.1"
                     /translation="MFRLAHISDVHLGPLPAVTYRDLASKRVVGYVNWQRNRRRHMHD
                     AVIDTIVADLKANGPDHLAVTGDLVNLALDGEIEMARLWLETLGSPQDVSVVPGNHDA
                     YVPGAFDKACRSWAAWMTGDGVNGTVDRHAFPYLRVRGDIALIGISTARATAPFMANG
                     FFQEGQAARLEKMLDETGRRGLCRVIMIHHPPVRGAVAQHKRLFGIGRFHKAIHRCGA
                     ELILHGHSHVPSLNYVGGQGARIPVIGVAAAGQAPGGSHVAAQYNLLDIEGAKGDWRV
                     RLVRRGLTGLALPPSELETAEFRLGVEASAVRN"
     gene            8036..8533
                     /locus_tag="VGM46_07580"
     CDS             8036..8533
                     /locus_tag="VGM46_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008877894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY4192492.1"
                     /translation="MDEAAEKAFRQTGWPGLRARLFHLYFLLRRPMTLGVRGLVFDRA
                     ANSVFLIRHTYVPGWQLPGGGVELGETMAEALGRELSEEGNIELTVPAKLKSVHFNRR
                     ASRRDHVGFYLIEDFVQSAPKQPDHEIAEAGFFPLDRLPDGITPATLKRIAEIFEGAA
                     ASPYW"
     gene            complement(8536..9525)
                     /locus_tag="VGM46_07585"
     CDS             complement(8536..9525)
                     /locus_tag="VGM46_07585"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY4192493.1"
                     /translation="MGLLVEGKWQDRWYDTKSTGGKFERSQSQWRDWITVDGTPAQGR
                     SRGFKAEPGRYHLYVSLACPWAHRTLIFRALKKLEGIISVSVVHPFMGADGWTFLAED
                     GATGDTLYGLDFLHQLYTRADPAYSGRVTVPVLWDKQEQTIVSNESSEIIRMLNSAFD
                     EWGDASLDLYPQALRGEIDTINALVYPAINNGVYRAGFATTQQAYEEAFQELFAALDQ
                     LDERLSKQRYLTGGQLTEADWRLFTTLVRFDPVYVGHFKCNLRRIADYPNLSNYLREL
                     YQVPGVAGTVNIQHIKAHYYGSHATINPTRIIPVGPALDYEAPHDRDRFAKAA"
     gene            9786..10301
                     /locus_tag="VGM46_07590"
     CDS             9786..10301
                     /locus_tag="VGM46_07590"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEY4192494.1"
                     /translation="MSTADVTYLPETPAHDPEIDTLNEQAFGPGRFARAAYRIREGGP
                     HDRGLSFVAMKEGAVIASVRMTPVAAGVGRAQMLGPLAVQPAFKNLGIGRRLVAIALE
                     QAAKAGVPAVLLVGDEPYYGPLGFTRVPHGQITMPRPVDLNRVLAHEIATGAVRRLSG
                     EVCHAAQAEAR"
BASE COUNT         1969 a         3408 c         3109 g         1898 t
ORIGIN      
        1 tgccggaggg cttcgaagtg accgccacca gcgaggatgg caccatcatg gccttcgaac
       61 accggcatga ggcggtcgca gccgtgcagt tccaccccga atcgatcatg tcgctcggcc
      121 acgatgccgg catgcgcatg attgagaacg tagtcgtgca tcttccgcgc aaagccaggg
      181 aaaaggcagc ctagccctta ctgcaatgac cgaaatcgat acccacaacg ccgccaggca
      241 gcgcgtcgcg accattgccg catggtcgat cgtcatcgca ttcatcgtgc tggcgctgaa
      301 gacgcttgcc tggttcgtca ccggttcggt cgccctttat tccgacgcgc tggaatcgat
      361 cgtcaacgtg atcgcggcct gcgccgccct gtgggcgatc cgcatcagcc acaagccggc
      421 cgataccgat catcccttcg gccaccacaa ggccgagtat ttttcagccg tactcgaagg
      481 cgtgctgatc gtcatagcag cgctgctgat cctcaccgaa gtgtggcgct cctggcagca
      541 tcacccgccg ctggaacagc catggagcgg ccttgccgtc aacggcgcgg cttccctgat
      601 caatgccggc tgggccttcc tgctgatacg caccggccgc gccgagaagt cgccggcgct
      661 ggtcgccgat ggccagcaca tcatgaccga cgtggtgacc tcgatcggcg tgtttgccgg
      721 tctggtcggc gccatcctga ccggctggca aatcctcgac ccgctgctcg ccgtgctggt
      781 cgccatcaac atcctgtggc agggctggca cgtcatcggc tcgtcgctca acgggctgat
      841 ggaccgcgcc gtcgatatgc cggaacacat gcgcatccgc gatatcatct cggccaattc
      901 gaaaggcgcg ctggaggttc atgacctcaa gacgcgcatc gccggccgcg ccacgttcat
      961 cgaattccac atggtcgtcg atgccgacat gacggtgagc gaaagccaca ccatctgcga
     1021 ccgcatcgaa gacgccctca aggccgatat tccctcggtg cgcgtcacca tccatgtcga
     1081 gccggacgac gaggcgaaac tgcccaaggg cagcgtcgcc gttcccttcg cctgattgcc
     1141 ctgccgggcg aagcctggcc tggccggaac gaaacatgcc ggtaacggct tgacgccgcg
     1201 cctcttcgcc aacaccatgg atgatcggag tgcgtggctg tccaacgccc tgacgacgtg
     1261 cacgtttcag ccaatctcgc tggggtaatc ggcagatacc ctggcgcggc gatgggagat
     1321 gacggatgta tggcctgatc ggcaagatgc gttcggcgcc gggcaagcgc gaagagctgt
     1381 tgcaaatcct gtttgaagtc accggccaat tgccgggatg cctcagctac atcgtcgcgc
     1441 gcgaccccgc cgacaccacc gcgatatgga tcaccgaagt gtggaaagac gccgtcagcc
     1501 acaaggcgtc gcttgccctg ccggagatac aggcggcgat catcaaggca aagcccttcc
     1561 tcgccggctt cgaattccag atcgaaaccg aacctgccgg cggctttggc ctgccgaaat
     1621 cctgaacccg caggctgggc gcggccggtg tcttcccggc tccccttgcc tggcctgaac
     1681 gcctcctgta agagagcggc catgaccaag cgccatgcat cgatcgctgc ccgtccgacc
     1741 ggctttgccg gcgagacgtt gcgcgcgctt gcctccacgc ttctgcttct cggccttatc
     1801 ggcgatcgcc gggtccgctg aaggagcgcc cggcggtcga gaaccgccgt caggcgccta
     1861 cgctccttgg caaagtcaca ttcagcgatt gaaaatctgg taaggcgctg ctgacaccga
     1921 aggccgcttg aagcgctttc gcgaagccgc ccggagacga agactatgag catgacgaac
     1981 gaaacgaacg cggcaggtac gaccgcgaag ggcatggcgg acgcggcacg caaatatgcc
     2041 ccctatccca cggtcgggct ttttgaccgc acatggccgt ccaaggtcat cgacaaggcg
     2101 ccgatctggt gttcggtcga cctgcgcgac ggcaaccagg cgctgatcga tccgatgggc
     2161 catgagcgca aggcgcgcat gttccagctg ctgctcgaca tgggcttccg cgaaatcgag
     2221 atcgggtttc cgtccgcttc gcagaccgat ttcgattttg cccgctggtg catcgaacag
     2281 ggtaacgtgc ccgccgatgt ttccctgcaa gtgctggttc agtgccggcc cgaactcatc
     2341 acccgcacct tcgaatcgct ggaaggcgct acccggccca tcgtgcattt ctacaattcc
     2401 accagcgagt tgcagcgccg cgtggttttt gaaaaggacg tgcgcggcat caagcgcatc
     2461 gccaccgacg ccgccaagat gattaccgac atggcggcga aggcggccag ggaaccggga
     2521 cattccggct atcgctttca gtattcgccg gaaagcttca ccggcaccga actcgaagta
     2581 gccctggaaa tctgcaacgc cgtcaccgag atcgtccagc cgacgcccga caacaagctg
     2641 ataatcaacc tgccgtcgac ggtcgagatg tcgacgccga acatctatgc cgaccgcatc
     2701 gaatggatgt gccggcaact cgacaaccgg gaaaacctgg tcgtctcgct ccacccgcat
     2761 aacgaccgcg gcaccggcat cgccaccacc gagcttggcc tgatggctgg cgccgaccgc
     2821 gtcgaaggca cgcttttcgg caatggcgag cgcaccggca atgtcgatat cgtcacgctg
     2881 gcgctcaata tgtacacgca aggtatcgat ccaaagctcg actgctcgga tatcaaccgc
     2941 atgaaggcgg tctacgaata ttccaaccag ttgaaaatac ccgagcgcca tccctatgtc
     3001 ggcgaactgg tctacacggc cttttcaggc tcccaccagg acgccatcaa caagggcatg
     3061 aaggcgctca aaaaggccaa taccgagctt tgggaagtgc cctacctgcc gatcgacccg
     3121 gccgatgtcg gccgcagcta cgaggcgatc attcgcatca attcgcagtc gggcaagggc
     3181 ggcatcgctt acgttttgca ggccgattac gggcttaacc tgccgcgcaa cctgcaaatc
     3241 gaattcagcc aggacatcca ggcgatcacc gatgccgaag gcaaggaagt gccggccagg
     3301 cgcatccacg agcagttcct cgaaacctat gtcgaccagc cgggagcgcg gctgaagttc
     3361 gtcgagcaca ccacctatcc cgacacgacg acgaagggac gccgcctcgt cgaatcgacc
     3421 attctcgaca atggccgcga agtcaccatc accggcacgg ggaccgggcc gatcgacggc
     3481 tttgtcgagg ctttgtcgcg ccatatcggc gtgccgatgt ccgtgctcga ctattccgaa
     3541 cattcgatgc agcgcggctc caatgcctcc gcgatctcct atgtcgaggt cgagcatccg
     3601 ggcggcaagc tgttcggcgc cggcataaac accaatattt cctccgcttc gctggaagcg
     3661 gtggtctcgg ccgccaaccg cattctgggc ggatgggcgc cgcgctgaat taaccatccg
     3721 cgcaatgctt gccgggataa ccggcaggca tttgtgtgga ttgcggatcg gcgcttgtgc
     3781 aaccgtacgg tggatatatg ttatgcttca actgtgccgc ctgtcgaaag gtatgacact
     3841 tgcagcattc cacgcctgcc gcccccgcag tgcgcgaaac gctggagcgc ctgctcacca
     3901 gcgagacgtt tggtcgttcc gaacgtgcgc gtgaacttct gcggtatctt gtcgagcgcc
     3961 agcaggccgg cgaagccgaa aggctgaagg gcttttccat cgccgtcgat gtcttcggaa
     4021 aggatatcga tttcgattcc tcgaccgatg cggtggtgcg cgtccaggcg ggccggctgc
     4081 gcgaactcct gaaccagtac ttcgccaccg agggcgcgtg cgatccggtt cggatttcca
     4141 ttccgcgtgg cagctacatt cccacctatg aactgaacgc aaagccgctc ctcgcggcgc
     4201 cgaacgacct ggcggaaagc ctcgtgcccg gcatgcccgc cgcgcagtcg cccgcgccag
     4261 accctggcct gaaccagaag gctacgccgt cgatcatatg gcagctgcgc tgcttctggg
     4321 cggcgatggc gcttatcatc gtcatgctcg gtgtcgtgat cgttcgccag gctatttttc
     4381 tggcccccga caatgaaccg gcagccaatg gcgagggcgg catttcgaca agcagcatcg
     4441 ctgcaccatc ctcgctggat gtgctgccct cggtctatat cgacatggaa accgacggtc
     4501 cgcaggccgg gcgtgtcggc gcgtcgctgc gcgcgggcct ttcctccttc aatacggtcg
     4561 atttcatagg ccgcgcagcg ccccggcagc ccgatccgga agccgatgca tcgagcttcg
     4621 ttttccacgt gctgcccgat ccttcggacg ggggcataac catcgaactc cagaacatgg
     4681 tcaccggcca ggtgcttctg tcgcgacagc tggaaaaggc ggaggcggag ccttccgcca
     4741 tcgaggaccg cattgccgga atcctgaccg ccaccctccc aacctccggg accatctata
     4801 attacatcga gcaaaacggc cttcagggcg gcctgaccca atgcctgctg ctggacgaaa
     4861 aatactatct cgaccaaaat cccgtcaccc acaagccggc ctatctttgc cttgaaaagc
     4921 tgatcgcgca gggcgtgaag tcgccgctgg cctattccga gatcgcgtcc ctgcacatgg
     4981 aagccgtgac caagcattac tcctaccccg tcggtgcaac agtggagcag gcaatcgcct
     5041 tggcccgcct cggtatgcgg atgggatcga ccagcgcttc ggtccaccgg tcatatggct
     5101 acatcaattc caggctcggc aggaccgacg ccgcgatccg cctgatgcgc aaggcctatg
     5161 aattgaacac ctacgacctc accatggccg ccgcctatgg ctatggcctg atcttcgcgg
     5221 gaaaatatgc gcagggaacc cctatcctcg cccgcgcggt cgaggcatcc agttcccatc
     5281 cgacatggtg ggatttcggc ctgttcgccg gcgagttcat gcaagggcat tcggccgaag
     5341 ccaagcgcgc cagcgacgcc ctgacgacga ccgagcccaa gtcgcattat ctggccgccc
     5401 gcatcatcag cgccaccgct tcaggcgatg gcgagcaggc gaaacagttg atcgacaagc
     5461 ttggcagcga attcccggac ttcgtggcca atccgcgcaa ggtttttctc gaacgaaact
     5521 atcccgccga tctcaccgac cagttggtga aagccctgcg tcaagccggc ctccccgatg
     5581 ccagccgatc gtaaggcccc gccagttctc cattcgcccg ttctccattc acttgaaagt
     5641 ggagcgggcc ggcaaaccat cgttttcgtg catggtttcg gcgcaaccca taatgtgtgg
     5701 gagaagatag ccgcgccgct gtcgcaagag gcgcggatca tcgcctatga cctgcccggc
     5761 cacggactgt cgctcgatgc gtcgggcgcg ggttcggcga aatctgccgc acgcgccatt
     5821 ctggccgatc tgtcggagcg cggcatcaag gcggcgcata tcgtcggcca ttcgatgggc
     5881 ggggcggtgg cgacgctgat ggcactggcc gagccgcagc gcatcgtcac cctcaccctg
     5941 ctcgcgccgg gcggctttgg acccgagatc gatggtccgc tgttgcgccg ttatgccaaa
     6001 gcagcggatg gggaagaaat ccgcgcttgt ctcgccgcca tgtccggcga aggtgctgca
     6061 atctccgatg acgttttgaa cctggacctt actatgcgca cccgccccgg ccagaccgcg
     6121 aaactggtcg aaatcgcggc ggcgatcacg cgcggcgacc gtcagggggt catcgcgcat
     6181 gagcacctcg cctcgctggc catgccggtc gccgttctgt ggggcacgca ggacccggtc
     6241 ttgcccttct tgcagaccgc cgcgatgccc gcgcatttta cccttcacgc cattcaggct
     6301 gccgggcata tgctgatcac ggaagcgccc gaaccggtgc ggcagacgat cgccttcaat
     6361 cttgccaact ctgtttgaac aggcggctgt aattcaccat cttctatggt aactcctcgt
     6421 taaccaaatt ccggggcaga tgccgatgaa agatgccagc gagaccgcaa gccccaccgg
     6481 ccagaccaga agtctgtccg ccgccttgcg cgacgtgaag aacgcgcttg ccgatcgcga
     6541 cgatgtcgtt gtcgacatgc gcgacgccca ccgtatgcgg ctggaacttc tggcggtgga
     6601 actggccccg gttttcaagg acgtgccggt cgacgtggac agtttcgact tcgtcgtttc
     6661 atccggcttg cagcccaggc tgtggatcga cgccgtcagc catgtggcga tggggcgcga
     6721 ccgccgcact taccgtttcc tcagggatac gcgcatcggc cgggtcgtgc tggctgaatc
     6781 gaccgacatg aagccggtcg ccgatcaggt cgcgcgctac gtcgccgagc gcatcgtcga
     6841 gcgccagcgc atgatggatg gcggtgctga accggcttct ggcggcttga tccgcgcggc
     6901 atggccggaa gccgagccgc cgctgcgcca aaccggacga aacaggttct ggcaggctct
     6961 tttcgccggg ctggcattga tcgtggccgg agcgctgttt gggcttggca tatcggcggt
     7021 gctgttttgg ggccgcatca gcgcggcgct attcagttcc tgacggccga agcctcgacg
     7081 cccagccgaa actccgcggt ttcaagctcg gatggcggca gggcaaggcc ggtcagcccg
     7141 cgccggacaa gccggacgcg ccagtcacct ttcgcgccct ctatgtcgag cagattatac
     7201 tgcgccgcca catgcgaacc gcccggcgcc tgtccggctg ccgcgacgcc gatgaccgga
     7261 attcttgcgc cttgcccgcc aacataattg agcgacggca catgcgaatg gccatgcagg
     7321 atcagttccg cgccgcaacg gtggatcgcc ttgtggaagc ggccgatgcc gaacaggcgc
     7381 ttgtgctgcg caacagcgcc gcgcaccggc ggatggtgga tcatgatgac ccggcaaagc
     7441 ccccgcctgc cggtttcgtc cagcatcttc tccagccgtg ccgcctggcc ctcctggaaa
     7501 aagccattcg ccatgaaagg cgccgttgcc cgtgccgtcg aaatgccgat cagcgcgata
     7561 tcgccgcgca cgcgcagata gggaaaagcg tggcggtcaa ccgttccgtt gaccccgtcg
     7621 ccggtcatcc atgccgccca cgaccggcag gccttgtcga aggcgcccgg cacataggcg
     7681 tcgtgattgc cgggcaccac cgatacgtcc tgcggcgaac cgagcgtctc cagccacagc
     7741 cgcgccatct cgatctcgcc gtccagcgcc agattgacca ggtcgccggt caccgcgaga
     7801 tggtcgggtc cattggcctt cagatcggca acgatggtgt cgatgacggc atcgtgcatg
     7861 tggcggcggc gattgcgctg ccaattgacg tagcccacca cccgcttgga ggcgaggtcg
     7921 cgataggtta cagcgggaag cggccccaga tggacatcgg aaatatgcgc gagcctgaac
     7981 atttcacctt cataggcgag gccggacctg ctttccaccg aaggtttgct tcctgatgga
     8041 tgaagccgcc gagaaagcct tccgccagac cggatggccg ggcctgcggg caaggctgtt
     8101 tcacctctat ttcctgcttc gccggccaat gacgcttggc gtgcgtggcc ttgtctttga
     8161 ccgggccgcc aattccgtgt tcctcattcg ccatacttac gttcccggct ggcagttgcc
     8221 gggcggcggc gtcgagctgg gcgagacgat ggccgaggcg ctcgggcgcg aactttccga
     8281 ggaaggaaat atcgaactca ccgtccccgc caagctgaaa tccgtccact tcaaccggcg
     8341 ggccagccga cgcgatcatg tcggcttcta tttgatcgag gatttcgtcc agtccgcgcc
     8401 caagcaaccg gatcacgaaa ttgccgaagc cggtttcttc ccgctcgacc gattgcccga
     8461 cggaatcacc ccggcgacgc tgaagcgcat cgccgagata ttcgagggag cggcggcctc
     8521 gccctattgg taggttcagg cagcttttgc gaagcggtcc cggtcatgcg gcgcttcgta
     8581 gtcgagtgcc ggcccgaccg gaataatgcg cgtcgggttg atcgtcgcgt gactgccgta
     8641 ataatgcgcc ttgatgtgct ggatattgac cgtgcccgcc acgcccggca cctgataaag
     8701 ctcgcgcaga tagttcgaca ggttcgggta gtcggcgata cggcgcaaat tgcacttgaa
     8761 atgcccgaca tagaccggat cgaaacgcac cagcgtcgtg aatagccgcc agtccgcctc
     8821 ggtaagttgc ccgccggtca ggtagcgctg tttcgacagc cgttcgtcaa gctggtccag
     8881 cgccgcgaaa agctcctgga acgcctcttc ataagcctgt tgcgtcgtcg cgaacccggc
     8941 gcggtagacg ccgttgttga tcgccggata gaccagcgcg ttgatcgtgt cgatctcgcc
     9001 gcgcagcgcc tgcggataga gatcgaggct ggcgtcgccc cattcgtcaa aggctgaatt
     9061 gagcatccgg atgatctcgg aagactcgtt gctgacgatg gtttgctcct gcttgtccca
     9121 caaaaccggc acggtgacgc ggccggaata ggcagggtcc gccctggtgt agagttggtg
     9181 caggaaatcg agaccgtaga gcgtgtcgcc ggttgcgccg tcctcggcaa ggaacgtcca
     9241 gccatcggca cccatgaacg gatgcaccac cgaaacggaa atgatgcctt ccagcttctt
     9301 cagcgcgcga aagatcagcg tgcggtgcgc ccacggacag gccagcgaaa catacaggtg
     9361 gtagcgccca ggctcggcct tgaagccgcg cgaacggccc tgcgccgggg ttccgtcaac
     9421 ggtgatccag tcgcgccatt gcgactgcga ccgctcgaac ttgccgccgg tcgatttcgt
     9481 atcgtaccag cggtcctgcc atttgccttc gaccagcaat cccatcgcca aacctctcgt
     9541 ttcacattcc ttgtagcaat aaatggcgtc accaacgcaa cttgccattg aacagagcgt
     9601 caagcgaccg cggcccgaaa acgccgattg cagcgaaggg catttggtgc tagcggaaaa
     9661 tcaccatggc ccatgcccca gcaacgatcc ggttcgaccg acgacgaaag agcgcccgcg
     9721 cctgaacagg ctggcgaacg ccgctttgcg cggcaccttt cctcgcatac cggaacgtga
     9781 cgaccatgag caccgccgac gtgacttacc tgccggaaac cccggcgcac gaccccgaaa
     9841 tcgacaccct taacgagcaa gccttcgggc cgggccgttt tgcgcgcgcc gcctacagga
     9901 tacgcgaagg cggcccgcat gatcgcggcc tgtccttcgt cgccatgaag gaaggcgccg
     9961 tcatcgcttc cgtgcgcatg accccggttg ccgccggcgt cggacgcgcc cagatgctcg
    10021 gccctttggc cgtgcagccg gccttcaaaa atctcggcat cggtaggcgg ctggtagcga
    10081 ttgcgctgga acaggcggcg aaagccggcg tgccggctgt cttgctggtc ggcgacgagc
    10141 cttattatgg cccgctcggc ttcacgcggg tgccgcacgg gcagatcacc atgccgcgcc
    10201 ccgtcgatct caaccgtgtg ctggcgcatg aaatcgcgac tggagccgtg cgcaggcttt
    10261 ccggcgaagt ttgccacgcc gcgcaggctg aagccagata ggccgcgttg cttccggcgc
    10321 tgcggctgcc tactgtgcga caaaccatga ggagacggca atgaacccga acggactggt
    10381 cgca
//
LOCUS       DASYCS010000236         3313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2376311,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000236 DASYCS010000000
VERSION     DASYCS010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2376311"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            297..1307
                     /locus_tag="VGM46_07595"
     CDS             297..1307
                     /locus_tag="VGM46_07595"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     exonuclease"
                     /protein_id="HEY4192495.1"
                     /translation="MRASDLLHPRPEGLYCPPGDFFIDPVRPVERALITHGHSDHARS
                     GHRSVLATRETLDIMALRYGEGFAGATQEAKLGQAIALGSVTVSFHPAGHVLGSAQIA
                     VEHKDMRIVASGDYKRRADATCRPFEVVACDVFITEATFGLPVFRHPPDSHEIARLLK
                     STEQFPQRAHLVGAYALGKAQRVMRLIRDAGYDKPIYIHGALARLSDYYQSQGIDLGI
                     LQPATVETGKKGDFAGAIVVGPPAAFADRWARRFPDPVSCFASGWMRIRQRAKQRGVE
                     LPLIISDHCDWDELTETIKQTGAGEVWVTHGREEALVRWCELEGIAAKPLHLVGYEDE
                     GD"
     gene            1312..2910
                     /locus_tag="VGM46_07600"
     CDS             1312..2910
                     /locus_tag="VGM46_07600"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020813205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cisplatin damage response ATP-dependent DNA
                     ligase"
                     /protein_id="HEY4192496.1"
                     /translation="MQRFAELLDRLVLTPARNGKLTLLTDYFRSVEDPDRGLALAAVT
                     GDLKIAAVKPAMLRALVAERMDPVLFGYSYDYVGDLAETVSLVWPQSPSAIPNHAPTL
                     ADVVEKLQTASRSDGPKVLAALLDSADIDARYAIIKLVTGGLRIGVSARLAKQAVADL
                     GPVDVAEIEELWHGLAPPYIELFAWLEGRAEKPERTAKALFRPVMLSNPVSDNDLEKL
                     DPADYAAEWKWDGIRVQAVSEGGVRRLYSRTGDDVSGAFPDLADAMDFSGTLDGELLV
                     GTPSVTGTFSDLQQRLNRKTVTPKIMQQYPAFMRCYDLLQQDGDDLRALSFRERRARL
                     EIFVKTLDPARFDLSPFVAFAGWPQLEALRATPPHPIIEGVMLKRWDSLYLAGRPKGP
                     WFKWKRDPHTVDAVLMYAQRGHGKRSSFYSDYTFGVWSGPAGAEELVPVGKAYFGFTD
                     EELKQIDKYVRDNTVERFGPVRSVRADRANGLVLEVAFEGLARSTRHKSGVAMRFPRI
                     SRLRWDKPAAEADRIETLKALLDG"
     gene            complement(2915..>3313)
                     /locus_tag="VGM46_07605"
     CDS             complement(2915..>3313)
                     /locus_tag="VGM46_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192497.1"
                     /translation="LVSAPADAKAEVMQDDTGSFLRIRSGASGSAISFDVGQGILEKL
                     AGKHAVFDVLARSEDGKDTQISIDCNFGDMGDCGRKRYAVGHERNEYLFDVTFPDKHP
                     GAAGTIAINSDFDRQGKAVDIYEIRVSAAQ"
BASE COUNT          560 a         1077 c         1079 g          597 t
ORIGIN      
        1 cgcgcaaatg cgcctcccgg cggtgaggca agaacaccgg cgggacgcga ggttcgcgcc
       61 acgtaactta tcactctgga aggctcggcc tcgcgccccg cttcccacct gcccctgcat
      121 aagggcagcg ttccttgaca acagccagac ggcgaatgcc gggcgtggca acgaggcggc
      181 tattcgccgg ccgcctgacg gatcgcgctg gacgacagcg gcgagcgcgg gccgtggatg
      241 gcaatagcgc gctgtcgcag tcttcccctt ggccgacaat cgtttatctt cgcgccatgc
      301 gcgccagcga cctgcttcac ccccggcccg aaggcctcta ttgtccgccc ggcgatttct
      361 tcatcgatcc ggttcggccg gtcgagcggg cgctgatcac gcacggccac tccgatcatg
      421 cccgctccgg ccatcgctcg gtgctggcga cgcgcgagac gctcgacatc atggcgctgc
      481 gctatggcga gggttttgcc ggcgcgacgc aggaggccaa gcttggccag gccatcgcgc
      541 ttggcagcgt cacggtcagc tttcacccgg cggggcatgt gctgggttcg gcgcagatcg
      601 cggtcgagca caaggacatg cgcatcgtcg cctccggcga ctacaaacgg cgcgccgacg
      661 ccacctgccg gccgttcgag gtggttgcct gcgatgtttt catcaccgag gcgacgttcg
      721 gcctgccggt attccgccat ccgccggata gccacgagat tgcgcggctg ctgaaatcga
      781 ccgagcagtt tccgcagcgc gcgcatctgg tcggcgccta tgcgctcggc aaggcgcagc
      841 gcgtcatgcg gcttatccgc gatgcgggct atgacaagcc gatctatatc cacggcgcgc
      901 tggcgcggct cagcgactat tatcaaagcc agggcattga tctgggcatt ctgcaaccgg
      961 ccacggtcga gaccggcaaa aaaggcgatt tcgccggcgc catcgtcgtt ggcccgcccg
     1021 ccgcctttgc agaccgctgg gcgcggcgct ttcccgatcc ggtttcgtgc ttcgcgtcgg
     1081 gctggatgcg cattcgccag cgcgccaagc agcgcggcgt cgagctgccg ttgatcatct
     1141 ccgaccattg cgactgggac gaactgacgg agacgatcaa gcagaccggg gccggggagg
     1201 tctgggtcac gcatggccgc gaggaagcgc tggtgcgctg gtgcgagctg gaaggcattg
     1261 ccgcaaagcc gctgcacctc gtcggctacg aagacgaggg ggattgagcc gatgcaaagg
     1321 ttcgccgaac tcctcgaccg gctggtgctg acccctgcgc gcaacggcaa gcttacactt
     1381 ctgaccgatt atttccgcag cgtcgaagac cccgaccgcg ggctggcgct ggccgccgtc
     1441 accggtgatc tcaagatagc ggcggtcaag ccggcgatgc tgcgcgcgct ggtggccgag
     1501 cgcatggacc cggtgctgtt cggctactcc tatgattatg tcggcgacct cgccgaaacc
     1561 gtctcgctgg tctggccgca atcgccgtcg gccatcccca accatgcgcc gacgctggcc
     1621 gacgtggtgg aaaagctgca aacggccagc cgctccgatg ggccgaaggt gctggccgcg
     1681 ctgctcgaca gcgccgatat cgatgcccgc tacgccatca tcaagctggt caccggcggc
     1741 ttgcgcatcg gcgtttcggc caggctggcc aagcaggcgg tggccgacct cggcccggtc
     1801 gatgtcgctg aaatcgagga attgtggcac ggattggcgc cgccctatat cgagcttttc
     1861 gcctggctgg agggcagggc ggaaaagccg gaaaggacgg ccaaggcgct gtttcgcccg
     1921 gtcatgctgt ccaatccggt cagcgacaac gacctcgaaa aactcgatcc cgccgactat
     1981 gccgccgaat ggaagtggga tggcatccgc gtgcaggccg tctcggaagg tggcgtgcgg
     2041 cggctctatt cgcgcaccgg cgacgatgtt tccggcgcat tccccgacct tgccgacgcc
     2101 atggactttt ccggtacgct ggacggcgaa ttgctggtcg gcacgccctc agtcaccggc
     2161 accttttccg acttgcagca acgcctgaac cgcaagacgg tgacgccgaa gatcatgcag
     2221 caatatccgg ctttcatgcg ctgctacgac ctgctccagc aagatggcga cgatttgcgc
     2281 gcgctgagtt ttcgcgagcg gcgcgcacgc ctggaaattt ttgtcaaaac cctcgatccg
     2341 gcccgcttcg acctttcgcc cttcgtcgcc tttgccggct ggccgcaact ggaagcgctg
     2401 cgcgccacgc cgccgcaccc gatcatcgag ggcgtgatgc tgaagcgctg ggattcgctt
     2461 tatctcgccg ggcggccgaa agggccgtgg ttcaagtgga agcgcgatcc gcacacggtg
     2521 gacgcggtgc tgatgtatgc gcagcgcggc cacggcaagc gttcgagctt ttattccgat
     2581 tatacgttcg gcgtctggtc aggcccggca ggcgcggagg aactggtgcc ggtcggcaaa
     2641 gcctatttcg gcttcaccga cgaggaactg aagcagatcg acaaatatgt ccgcgacaac
     2701 acagtcgagc gcttcggccc ggtgcgttcg gtgcgcgccg accgcgccaa tgggctggtt
     2761 ctggaagtgg cgttcgaagg gcttgcccgc tcgacacggc acaaatccgg cgtcgccatg
     2821 cgctttcccc gcatctcgcg gctgcgctgg gacaagccag cggccgaagc cgaccgcatc
     2881 gagacgctca aggccctgct cgacggataa gatgctattg cgccgcagag acgcggattt
     2941 cgtagatatc gaccgccttg ccctgccggt cgaaatcgga attgatggcg atggtgccgg
     3001 cggcgcccgg atgcttgtcg gggaaggtca cgtcgaacag atattcgttg cgctcatgcc
     3061 cgacggcgta gcgcttgcgg ccgcaatcgc ccatgtcgcc gaaattgcag tcgatggata
     3121 tttgcgtgtc cttgccgtcc tcgctgcggg caagaacgtc gaagacggca tgcttgccgg
     3181 caagcttttc caatatgcct tgcccgacgt cgaagctgat ggccgatccc gatgcgccgg
     3241 aacggatgcg caggaacgag ccggtatcgt cctgcatgac ttctgccttg gcgtcggcgg
     3301 gcgcgctgac cag
//
LOCUS       DASYCS010000237         1929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2090296,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000237 DASYCS010000000
VERSION     DASYCS010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2090296"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="VGM46_07610"
     CDS             complement(<1..521)
                     /locus_tag="VGM46_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY4192498.1"
                     /translation="MNDLIATAPVQPAKGVFIDNRWQPSKSGRLIDVYAPAEGAVFAQ
                     IAAGGAEDIDAAVKAARKAHEGAWGRLTATERGRLLSKLAQAILDHADELTLIEARDT
                     SKPLKQARADILTCARYFEFYGGAADKFHGETIPYMNGYFVATEREAHGVTGHIIPWN
                     YPAQMFARTIAPAL"
     gene            complement(589..1425)
                     /locus_tag="VGM46_07615"
     CDS             complement(589..1425)
                     /locus_tag="VGM46_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006326027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HEY4192499.1"
                     /translation="MSDESPNRFADWIGRASRSRDIVTERLDAGFRAVLAPHLAATGT
                     GQAPLGLHWCLCQPAAPMDELGPDGHPAKSRDLPPVPLPRRMWAGGQVETFDALRIGD
                     TVTRTSTIAGVTHKQGRSGELWFVAISHEYETGRGLAIRERQDLVYRDAASATPPKAN
                     PESPAPRPGTRHWSVQTSPVLLFRYSAITFNSHRIHYDHPYATGVEGYDGLVVHGPMQ
                     ATLLLNLAAAQAGETPRRFSYRGVFPAMEGRELMVCRDDGGHDYWTESGGRIHMEAKA
                     EF"
     gene            complement(1446..>1929)
                     /locus_tag="VGM46_07620"
     CDS             complement(1446..>1929)
                     /locus_tag="VGM46_07620"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY4192500.1"
                     /translation="VGEEGKGFRYILSGMNAERILIAAECVGDAKWLIEKASLYASQR
                     NVFGRPIGANQGIQFPIARAYANMRAAELMVKEAARLYEQGKDCGAEANMAKMLAADA
                     SNEAANACIQTHGGFGFAEEYDIERKFRETRLYQVAPISTNLILSFLSEHVLGMPRSY
                     "
BASE COUNT          284 a          623 c          665 g          357 t
ORIGIN      
        1 agcgccggcg cgatggtgcg cgcaaacatc tgcgccggat agttccaggg gatgatatgg
       61 ccggtcacgc catgcgcctc gcgctcggtg gcgacgaaat agccgttcat gtaggggatc
      121 gtctcgccgt ggaatttgtc ggccgcgccg ccatagaact cgaaatagcg tgcgcaggtg
      181 agaatgtcgg cgcgcgcctg cttcaggggt ttgctggtgt cgcgcgcctc gatgagcgtc
      241 aattcgtcag catggtcgag gatcgcctgc gcgagtttcg acaggaggcg gccgcgctcc
      301 gtcgcggtga gccggcccca ggcgccttca tgggccttgc gcgcggcctt caccgccgcg
      361 tcgatgtctt cggcgccgcc ggcggcgatc tgcgcgaaca ccgcgccttc ggccggcgca
      421 tagacgtcga tgaggcggcc cgacttcgac ggctgccatc ggttgtcgat gaacacgccc
      481 ttggccggct gtacgggcgc ggttgcgatt agatcgttca tgtctctgct tcctcacatt
      541 tgcctggtcc cgctcgccgc gcatgcggtg ggcggacggc tggcgtcgtc agaattcggc
      601 cttcgcttcc atatggatgc ggccgccgct ctcggtccaa taatcatgcc cgccatcgtc
      661 gcggcatacc atcagttcgc ggccttccat ggccggaaaa acgccgcgat agctgaagcg
      721 gcgcggcgtt tctcccgctt gtgccgccgc caggttgagc agcagcgtcg cctgcatggg
      781 gccgtgcacc accagcccgt catagccctc gacgcccgtg gcatagggat ggtcgtagtg
      841 gatgcggtgg ctgttgaagg tgatggcgga atagcgaaag agcagcaccg gcgaggtctg
      901 gacgctccag tgccgggttc ccggacgcgg tgccgggctt tccgggtttg ccttcggcgg
      961 ggtggcgctt gcggcgtccc ggtagacgag atcctgccgc tccctgatcg ccaggccgcg
     1021 cccggtctcg tattcgtgcg agatcgcgac gaaccaaagc tcgccgctgc gcccctgctt
     1081 gtgcgttacc ccggcgatgg tggaggtgcg ggtgaccgtg tcgccgatgc gcagcgcgtc
     1141 gaatgtctcg acctgcccgc ccgcccacat gcggcgcggc agcggcaccg gcggcaggtc
     1201 gcggcttttg gcggggtgcc cgtccggccc cagttcgtcc atcggcgccg ccggctggca
     1261 gaggcaccaa tgcaggccga gcggcgcttg cccggtgccg gtagcggcca gatgcggggc
     1321 aagcacggcg cggaagccgg catccagccg ctcggtgacg atatcgcgcg agcgactggc
     1381 gcggccgatc cagtctgcaa agcggttggg agattcgtcg gacattccag tgttctcaca
     1441 agccctcaat aggaacgcgg catgccgagc acatgctcgg acaggaacga caagatgagg
     1501 ttggtcgaga tcggcgcgac ctggtagagc cgcgtttcgc ggaacttgcg ctcgatgtcg
     1561 tattcctcgg cgaagccgaa gccgccatgc gtctggatgc aggcattggc cgcctcgttg
     1621 gaggcgtcgg ccgccagcat cttggccatg ttggcctccg cgccgcaatc cttgccctgt
     1681 tcgtagaggc gcgccgcctc cttcaccatc agctcggcgg cgcgcatgtt ggcgtaggcg
     1741 cgcgcgatgg gaaactggat accctggttg gcgccgatgg gccggccgaa cacgttgcgc
     1801 tggcttgcat agagcgaggc tttctcgatc agccatttgg cgtcgcccac gcattcggcg
     1861 gcgatcagga tgcgctcggc attcatgccg gaaagaatgt agcggaagcc cttgccctct
     1921 tccccgacc
//
LOCUS       DASYCS010000238         2035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4262835,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000238 DASYCS010000000
VERSION     DASYCS010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4262835"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="VGM46_07625"
     CDS             <1..678
                     /locus_tag="VGM46_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006338276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY4192501.1"
                     /translation="APPVFFGELPIEDRLYAETMCRVLHLRNFHNIGVPGLDLFFNYN
                     PQINDHPQRALAEIRLMAMHLDELDIDPRMLVCEITEQAAPDDRVLARLVSEMRRTGI
                     RIAVDDFGSGHSTTRRMSVVEPDIVKLDGQWFAQLCSHAEAEKLFKPLVATLHARGIK
                     VLVEGIETAHHLRVALGGDVDLLQGFHLARPALAGTFFDENPLMVQRLLGAPAALLPF
                     RATYQRR"
     gene            753..1367
                     /locus_tag="VGM46_07630"
     CDS             753..1367
                     /locus_tag="VGM46_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY4192502.1"
                     /translation="MTVLYGMTDSGNCYKPRLLMAKLGHAFTNVEVSSHTGDTRKAEY
                     LAKNPNAMVPLLELDDGRRIAESNAILLYLAEGTRFLPADRYQRAMVYQWLFFEQYSH
                     EPYIAVRKALLTFPERAGDATPERLAQTLERGNKALGVMNRHLGDNRFFAGDALSVAD
                     LALYAYTHDAEKGGFQIDAYPAVASWLKQVEADPGHVKIGWLPG"
     gene            1422..>2035
                     /locus_tag="VGM46_07635"
     CDS             1422..>2035
                     /locus_tag="VGM46_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY4192503.1"
                     /translation="MILIGQYDSPFVRRVGIAMRLYGLEFEHRPWSTFGDAEQIRPFN
                     PLMRVPVLVLDSGEALVESHGIVDYIDRLAPADRVLFPLDQPARYQALRIAALATGFA
                     EKSVSLFYEQRLHSDVSDLWVKRCVSQISGTLAVLEADRAARHSEYWFGDRIGHADIA
                     VATAFRHMKDAHPGLFGQHEFPALAAHSKRLEALPVFQEIQQVF"
BASE COUNT          380 a          642 c          618 g          395 t
ORIGIN      
        1 gcgccgccgg tcttctttgg cgaactgcca atcgaggatc gcctctatgc cgaaaccatg
       61 tgccgcgtgc tgcatctgcg gaacttccac aatatcggcg ttcccggtct cgacctgttt
      121 ttcaactaca atccgcagat caacgatcat ccgcaaaggg cgctggccga aataaggctg
      181 atggccatgc atctcgatga actcgatatc gacccccgca tgcttgtctg cgaaatcacc
      241 gagcaggcag cgcccgacga ccgtgtgctg gccaggctcg tcagcgaaat gcgccgcacc
      301 ggcattcgca tcgccgtcga cgatttcggg tcgggccact cgacaacccg gcgcatgagc
      361 gtggtggaac ccgatatcgt caagctcgac gggcaatggt ttgcccaatt gtgcagccat
      421 gccgaggccg agaagctttt caagccgctc gtcgccacgc ttcatgcccg tggcatcaag
      481 gttctggttg aaggcatcga gaccgcgcat catctgcgcg tcgcgctggg gggcgatgtc
      541 gatctgctgc aaggcttcca cctcgccagg ccggcgctgg ccggcacgtt cttcgatgaa
      601 aacccgctta tggttcaacg cctgctgggc gcgccggcgg cgctcctgcc tttccgggcg
      661 acctatcagc ggcgatgatg gtcccagcaa taaaaatcac tggataaagc ctgcctgcaa
      721 gcgcagtaat tgcccgcttc gagggcgacg cgatgactgt tctttacgga atgaccgaca
      781 gcggcaattg ctacaagcca cggctgttga tggccaagct tggccatgcc ttcaccaatg
      841 tcgaggtcag ctcgcatacc ggcgatacgc gcaaggcgga atatctggcc aaaaacccca
      901 acgccatggt gccgctgctg gaactcgacg atggcaggcg gatagccgag tccaacgcca
      961 tcctgctcta ccttgccgag ggaacgcgct tcctgccggc cgaccggtat cagcgcgcga
     1021 tggtctatca gtggctgttc ttcgagcaat acagccacga gccttacatc gccgtgcgca
     1081 aggcgctgct gacctttccg gagcgcgccg gcgatgcgac gcccgaacgg ctggcgcaga
     1141 cgctggaacg cggcaacaag gcgctcggcg tcatgaaccg gcatctcggc gacaaccggt
     1201 tctttgccgg ggatgcgctt agcgtcgccg acctcgcgct ctacgcctat acgcatgacg
     1261 ccgagaaggg cgggttccag atcgacgcct atcccgctgt cgcaagctgg ctgaaacagg
     1321 tcgaagccga ccccggccat gtgaagatcg gctggctgcc cggctgatac gccctcgggc
     1381 ttttgctatc gcgcgcgccc cgcatccgga ggttctagac catgatcctg attggccagt
     1441 acgattcgcc ctttgtgcgc cgtgtcggaa ttgccatgcg gctctacggg ctggagttcg
     1501 agcatcggcc gtggtccacc ttcggtgatg ccgagcagat caggccgttc aacccgctga
     1561 tgcgcgttcc agtgctggtg ctcgacagcg gcgaggcgct ggtcgaaagc catggcatcg
     1621 tcgattacat cgacaggctg gcgcctgccg accgggttct gtttccgctc gaccagccgg
     1681 cgcgctatca ggctttgagg atagccgcgc tggcaacggg ctttgcggaa aagagcgtca
     1741 gcctgtttta cgagcagcgg ctgcacagcg atgtttccga tctctgggta aagcgatgcg
     1801 tttcgcaaat atcaggcacg ctggccgtgc tggaggccga ccgcgccgcg cgccatagcg
     1861 aatactggtt tggggaccgt atcgggcatg ccgacatcgc ggtggcgaca gcatttcgcc
     1921 acatgaaaga cgcgcatccg gggctgttcg ggcagcacga atttccggcc cttgccgcgc
     1981 attcgaagcg gcttgaagcg ctgccggtat tccaggaaat ccagcaggtc ttcat
//
LOCUS       DASYCS010000239        13510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4698283,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000239 DASYCS010000000
VERSION     DASYCS010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 13510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13510
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4698283"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VGM46_07640"
     CDS             complement(<1..284)
                     /locus_tag="VGM46_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019858718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY4192504.1"
                     /translation="MAEFPAKARVVIIGLGGIVGASIAHHLIERGWDDIVGIDKSGIP
                     TDIGSTAHASDFCYMTSHDFLSCWTTLYSVDFYEKMGHYARVGGLEVARVG"
     gene            506..2275
                     /locus_tag="VGM46_07645"
     CDS             506..2275
                     /locus_tag="VGM46_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192505.1"
                     /translation="MSATFDKSADADGTQDKSLLRSPLALLAISLVAVCVLLSLPLVL
                     PIGPMYWDVFIYYDAANRIFDGQVPINDFFTPVGPLGYYLFAGWLAIFPNAQPALLAH
                     WSLLVVTAPLMALVTWHVDKRSRGTAFALLIPFLIFALLPFNTRELYSYPGSDAFGIY
                     NRQACQVLYIMVAALVFVRSFRLLATIIVATMTALFFLKITGFVAGGFIAAYALISGK
                     VTWKHAILAAAAFLLVLGGIELMNGLVSQYVIDITKLVQMNSDTLLPRFLQATSLNFG
                     IVASATALALVLLWSDARNLAKTFSSSWKASPLGASSALLAHDAIWLLVVLFAGILFE
                     TQNTGSQALIFLWPVLLSILLKTGKIADRPVLVFSIMALVAAAALPPTMRLTERAART
                     YAGSFKNVPLEARNLKSLGAVDMRRDVAVRTDHMLRFYPDHRTFYDDLVVAGELPTPL
                     LFSDFDFQVAYLSTVDTAIDSIRALETKAGVHFDTIMSLNFVNPFPWLMDRSAPRYVA
                     IGADPTRAVPAPGSLEEDAVSKTDLVLYPTCPPTTANALLYDMYSDALAKHRRIRLDA
                     CYDAFINPKFALPPGSTTAPSAQ"
     gene            complement(2322..3752)
                     /locus_tag="VGM46_07650"
     CDS             complement(2322..3752)
                     /locus_tag="VGM46_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY4192506.1"
                     /translation="MVNAPALSPATTPKPNLIGVEQAKAMDVARMTDLFKAHINPGQL
                     HFMKLLGFHKIKIERAEGMFYIDQNGRKILDFFGGFGSLAFGHNHPRILEARKSFQEE
                     KRQEIAIAFMSQYAAALAHNIAACSPGDLDMVFLGSSGSEAMEAAVKLAERAAGAKRP
                     KIVYAENSFHGKTKGVLAITDGSLYRGDFKLTDNVVKVPFADIDAVERAFQSDPQIGS
                     IVLETIQGGGGIIQAPAEYWQKLRALCDRYGVLWVADEVQCGYGRSGKFYAFEHYGVI
                     PDITALAKSLGAGKAAVGAMIARREVYMKAYGTPKTAMIHAQATFGGMGEACVTAIEG
                     LNVLYDEGLIDNAASTGDYLLARLQGLKEKHPKIIKDVRGKGFMVGLEFHDFSQTLPM
                     VLRPVVSVLDDKLKGSLSGFVGALLLRDYDVLVAFTEYNRNVIRLEPPLICQREHVDS
                     FIEALDSLLSRGIVAIVKDFVKSQMR"
     gene            complement(3855..4862)
                     /locus_tag="VGM46_07655"
     CDS             complement(3855..4862)
                     /locus_tag="VGM46_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY4192507.1"
                     /translation="MRHVIFGGDGFVGRHLAPKLVADGEEVIVADIVKSELSHYRSVR
                     HIHCDVTDPASVAGVGLKADDMVYNLSAKMLSPIQVRSKRHDFFFPVNFHGTEHIIQA
                     MDKAGAPKLVHFTTDMIYGHTVTYPMTEDHPVAPLGEYGWSKQKTEELAAEWRKRGMA
                     ISLFRPRLIIGPGRLGILEKLFKLIDWNMPVPMIGSGKNPYQFISVFDCAEACRAAWK
                     AGVPNEAYNLGSLNPPPVKRLLGDLIKHAGSKSLLVPTPGWAVKRTLDLLDAMNMPIM
                     DPEQYLIADEECVLDVSKAERQLGWAPKYRDEDMLIAAYSEYRAKKDGHAVAASHVPA
                     E"
     gene            complement(4859..5293)
                     /locus_tag="VGM46_07660"
     CDS             complement(4859..5293)
                     /locus_tag="VGM46_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY4192508.1"
                     /translation="MKYIAFILFTVMTNAAAQLMLKQGMISLNPISFEGANPVVKILQ
                     IVFSPWVFLGLCTFVISMASHLYVLSKVELSFAYPFLSLAYVAVAVFAYFLFREDLNG
                     WRIAGIACICVGTVLIAQSGRGHDDETASLSTDKIITSEIVR"
     gene            complement(5416..6768)
                     /locus_tag="VGM46_07665"
     CDS             complement(5416..6768)
                     /locus_tag="VGM46_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY4192509.1"
                     /translation="MRAAYSSFGRATPSAANALPADEAIALLRGGGAGEASLLAYGNG
                     RSYGDSCLNGDGALVDMRSLDRIISFDAEAGMIEAEAGVLLSDIIAYAAPHGFFPAVV
                     PGTQFVTLGGAIANDIHGKNHHRRGTFGCHVEGFTLLRSDGREYRCSASRNPHLFAAT
                     IGGMGLTGLILSASIRLMRVPSLDITETVRPFREIGEFFDLAMAADEANEYAVAWIDQ
                     LSGGQQFGRGLMLAGNHAEHGSHIACRPGGRLAVPFQPPLNVLNRPFLTAFNAAYRWK
                     NGRNTQPRHASYQGFFFPLDGVRDWNRLYGPRGLFQHQSVVPENVAREAVPALLRAAK
                     RAGQGSFLTVLKRFGSIASPALLSFPRPGYTLTLDFPNRGEATLKLLEELDRITVDAG
                     GAVNPYKDARMGPETFAASFPAWQRLEAVRDPAFMSSFWVRTAKRLAGSSSGLVEAAE
                     "
     gene            complement(6770..8203)
                     /locus_tag="VGM46_07670"
     CDS             complement(6770..8203)
                     /locus_tag="VGM46_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEY4192510.1"
                     /translation="MDARADRTAIPLAVDLDGTLIATDLLWEGLFALLRKNPLYLFLV
                     PFWLAGGPAKLKQAIADRVDIDPEALPYRQDLLARLRADHADGRKLVLATGTPRKFAE
                     AIAAHLGVFDAVLATEGGENLTSAKKRAALVAAYGDGGFDYAGNSRHDVAVFDAARAA
                     IVVAPDRKAALWQSAHGSEIMPAPRRSLKSILKMLRVHQWAKNTLIAVPMVLSHEYLN
                     AAMIVQCVFAFISFSAVASAIYIVNDFFDLALDRKHPTKCRRPFASGVLSIPFGLASI
                     CVLLAIGIGAGLLVSWPFMGVLAAYIVTTTAYSLTLKRMLLIDVLTLAGLYTVRVIAG
                     AAATGVGVSFWLLAFSMFFFLSLALVKRYVELGLPSLTPGERIAGRGYRSEDREVVAQ
                     AGMASAFSSALVLALYMDSDAVRELYPNPWMVWPLAPIVLYLTMRVWILGRRGEMHED
                     PVVFIISDWRSQIVIGLGAVLLVIGGW"
     gene            complement(8421..8684)
                     /locus_tag="VGM46_07675"
     CDS             complement(8421..8684)
                     /locus_tag="VGM46_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018428406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192511.1"
                     /translation="MLKSVVLAGMLVAGASSAYAASAVNNDGETRTLIVTEGGSKSEL
                     ALASGETAEFCPKGCFVTLPNGDLTPLTGSETVEISGGVAHIK"
     gene            complement(8838..11315)
                     /locus_tag="VGM46_07680"
     CDS             complement(8838..11315)
                     /locus_tag="VGM46_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEY4192512.1"
                     /translation="MLQRFYRFAVVLALLALLPSGAAAQSVSETANGLVAAQQQVIQQ
                     LTAKTDDLEKQMQQNGQDDAALVEIRLKLEGLSQRSLESALAFRSSTADINARLEQLG
                     PGPAEGQPAEPDIVTAERDALIAGKAQINGVIATAQTLSIRISGLIEQIGAIRSDLFR
                     NLLTKRYTLADTLSGKVLADAKAEMVRFYGAFSSWFSFALKFKFQAMLAATFMALGLA
                     AVLIVGGRRVFGRMFVADPTVDDPSYLSRLSVTFWSTLLPTAALVVFLVSTIFFFNYY
                     NVLRGDIGVFLNAFAALIAVIFCVNRLANAALSPRLPNWRLIPVESTPGRWLVRLATA
                     MALVIGGNTFLSIVNDQMNSPLSLTIARSFLATIVFGVILILMALVRPFCTTDGVWKP
                     WPAWLRYSAFALGAFTIGAALLGYIGLAIFVSLQVVVTGTILLTAYIGFLSARAIGAE
                     GGFGSSSIGRWLTEKGNVEEATLDQLGLAVSVALNLMIVVIFLPLILLMWGFQLGDIQ
                     AWGYKLATGLTIGSATISVTGILSGIVVFVIGYFLTRWFQGWLDGSVMARGKVDTGVR
                     NSIRLAVGYAGVAVAALVGISAAGIDLSSLALVAGALSLGIGFGLQNVVSNFVSGLIL
                     LAERPFKVGDWIVAGDVSGTVKKISVRATEIETFQRQSVILPNSNLINNAVGNWTHRN
                     KLGRVEIKVGVAYGMDFRRAHEIMLEIARAHPLVLKNPEPFVLFANFGPAALEFEVRV
                     FLADITDGSAVQNDIRFSILEMFDKEHIEIPSTARAVVAVEKHDPWPEDDDKTEVEFA
                     EQQQAKADAAAKRNSGRGRKSRKPDPG"
     gene            11502..12761
                     /locus_tag="VGM46_07685"
     CDS             11502..12761
                     /locus_tag="VGM46_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit beta"
                     /protein_id="HEY4192513.1"
                     /translation="MAEYSALSLLKNAFTGNKDWKPAWRKPTPKPSYDVIVIGGGGHG
                     LSTAYYLAKEHGITNVAVLEKGWLGSGNVGRNTTAVRSNYLLPPNTRFYEHSMKLWEG
                     LSHDLNYNVMFSQRGCLNLAHTPAQLDDYARRGNAMRHQGVDAELMTPAQIKRLIPAL
                     DVSDAARFPVYGGLMQPRAGTARHDAVAWGYARGADRRGVDIVENCEVTGFLRDGDRI
                     VGVTTTRGEIRARKVAVAVAGSTGRVMQLAGIDTMPIESHVLQAFVTESLKPFIDTVV
                     TFGMGHFYMSQSDKGGLVYGGDLDGYNSYAQRGSLPVVEEVMSEMLALFPGLAGVRLL
                     RSWGGVCDMSMDGSPIITTGPLPGMYLNCGWCYGGFKATPASGWCFAHTIAKDAPHEL
                     NAAFTLDRFHRGILIDEKGQGATPRLH"
     gene            12771..13067
                     /locus_tag="VGM46_07690"
     CDS             12771..13067
                     /locus_tag="VGM46_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015317398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta family protein"
                     /protein_id="HEY4192514.1"
                     /translation="MLITCPYCGPRDVSEFTYQGDGNRERPQPASQNLDAWNEYVYER
                     LNPAGDHNEIWQHSGGCRTHVRVVRNTLTHVISSAIPARAEARHKAASRRGAKS"
     gene            13064..>13510
                     /locus_tag="VGM46_07695"
     CDS             13064..>13510
                     /locus_tag="VGM46_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY4192515.1"
                     /translation="MSPRRALAGGSIDRLKTIRFTFDGTAFTGHAGDTLASAMLANGV
                     SLFGRSFKYHRPRGVLTAGVDEPNALVTILRGEVREPNIPATMIEIFDGLAVESQNRF
                     PSLAWDVNAVNQLGGKLLSAGFYYKTFMGPAIGPLKGTGFWMFCEKF"
BASE COUNT         2709 a         4275 c         3980 g         2546 t
ORIGIN      
        1 ccgacgcggg cgacctccag gccgccgacc cgtgcgtagt gccccatctt ctcgtagaaa
       61 tcgacggagt aaagcgtcgt ccagcaggac aggaagtcgt ggctggtcat gtagcagaag
      121 tcggaagcgt gcgccgtcga accgatgtcg gtcggtatgc cggacttgtc gatgccgacg
      181 atatcgtccc atccgcgctc gatcaggtga tgggcaatcg acgcgcccac gatgccgccc
      241 agcccgatga taacgacgcg cgcctttgcc ggaaactctg ccattctcgt cactccgaaa
      301 tagaattgca ggcacactat agagcggtgc gtttcgatta aagcctgttt gcgacacggc
      361 gcacatgcgg cgcgacttgg acctgaaccg cttcgctgaa gacgtgtttg cccgattgcg
      421 ccaaggttaa ctttttgctt gtttttttct gataggccca agccatgatc gggaatttga
      481 tcccgcaacg gcgcgggggc tggcgatgag tgcgactttc gacaagtccg ccgacgccga
      541 cgggactcaa gacaagtcgt tattgcggtc gccgctggcg cttctcgcca tctcgctcgt
      601 tgccgtctgc gtcctgcttt ccctgccact cgtcctgccc atcggcccga tgtactggga
      661 tgttttcatc tactacgacg ccgccaacag gatttttgac ggccaggtgc cgatcaacga
      721 ctttttcacg ccggtcggtc cgctcggcta ttatttgttt gcaggctggc tggcgatttt
      781 ccccaatgcg caaccggcat tgcttgccca ctggtccctg ctggtcgtca cagcgccgtt
      841 gatggcgctt gtcacctggc atgtggacaa gcgctcacgc ggcaccgcct tcgccctgct
      901 gatacccttc ctgatcttcg ccctgctgcc gttcaacacg cgcgaactct attcctatcc
      961 aggcagcgat gcctttggca tctataacag gcaggcctgc caggtgcttt acatcatggt
     1021 tgcggcactg gtgttcgtgc gcagcttccg gttgctggcg acgatcatcg ttgcaacgat
     1081 gacggccctg ttttttctca agatcaccgg cttcgttgcc ggcggcttta tcgctgccta
     1141 tgccctcatc tccggcaagg tgacatggaa gcatgccatc ttggccgcag cggcgttcct
     1201 gctcgttctc ggcggcatcg aactgatgaa cggcctggtc agccaatatg tcatcgacat
     1261 cacgaaactg gttcagatga acagcgacac gctgttgccg cgttttcttc aggcgacatc
     1321 gctgaatttc ggcatcgtgg cttcggctac cgcgcttgcg ctggtgcttc tgtggtccga
     1381 cgcgcgcaac ctcgcaaaga ccttctcttc cagctggaaa gcgtcgccgc ttggcgcctc
     1441 tagcgcgctt ctggcgcatg atgccatctg gctgcttgtc gttctgtttg ccggcatcct
     1501 gttcgaaacg cagaataccg gcagccaggc gctcattttc ctttggccgg tactgctttc
     1561 catcctgctc aagaccggaa agatcgccga ccgcccggtg ctggtatttt cgatcatggc
     1621 ccttgtggct gcggccgcgc tgccgccgac catgcgcctc accgaacgcg cagccaggac
     1681 atatgccggc tcattcaaga atgtgccgct ggaagcgcgc aatctgaaaa gcctgggcgc
     1741 agtagacatg cgccgcgacg tcgcggttcg caccgatcat atgctgcgtt tctaccccga
     1801 tcaccgcacc ttctacgatg accttgtcgt ggccggcgag ttgccgacgc cgcttttgtt
     1861 ttccgatttc gatttccagg tcgcctatct cagcacggtc gacacggcca tcgattccat
     1921 ccgtgcgctc gaaaccaagg caggcgtgca tttcgacacg atcatgtcgc tgaatttcgt
     1981 caatcctttt ccgtggctga tggaccgctc ggctccccgc tatgtcgcca tcggcgcaga
     2041 cccgacccgc gccgtgcccg cgcccggttc gctggaggaa gacgcggtat cgaaaaccga
     2101 tctggtcctc tacccgacct gcccgcccac gactgccaat gccctgctct acgatatgta
     2161 cagcgacgcc ctggcaaagc atcggcgcat aaggctggac gcctgctacg acgcctttat
     2221 caacccgaaa ttcgctttgc ctccaggctc gactaccgca ccgtccgcac aataaaaaaa
     2281 agccgggcga acccggcttt ttttcgtctg cctagccagc gctaccgcat ctggcttttc
     2341 acaaaatcct tcacgatggc aacgatgccg cgcgacaaca gactgtccag cgcctcgatg
     2401 aaactgtcga catgctctcg ctggcaaatc agcggcggct ccaggcgaat gacgttgcgg
     2461 ttatactcgg tgaaggcgac cagcacgtcg tagtcgcgca acagcagcgc cccaacgaaa
     2521 cccgatagcg aacccttgag cttgtcgtcc agaacgctga cgaccggccg cagcaccatg
     2581 ggtagcgtct gcgagaaatc gtggaactcc agcccgacca tgaagccctt gccgcgcacg
     2641 tccttgatga tcttggggtg cttttccttc agcccctgca agcgagccag caggtaatcg
     2701 ccggtcgaag ccgcattgtc gatcagccct tcgtcataga gcacattgag gccttcgatg
     2761 gcggtgacgc aggcttcgcc catgccgcca aaagtcgcct gcgcatggat catcgccgtc
     2821 ttgggcgtgc cataggcctt catatagacc tcgcgcctcg cgatcatcgc gccaacggcc
     2881 gccttgcccg cgccaagcga cttggcgagc gcggtgatat cgggaatgac gccataatgc
     2941 tcgaaggcat agaacttgcc cgagcgccca tagccgcact gcacttcgtc ggccacccac
     3001 aacacgccat aacggtcgca cagcgcgcgc agcttctgcc agtattctgc cggcgcctgg
     3061 atgatgccgc cgccaccctg gatagtctcc agaacgatag agccgatttg cgggtccgac
     3121 tggaaggcgc gctcgaccgc gtcgatatcg gcgaacggca ccttcaccac attgtcggtc
     3181 agcttgaaat cgccgcgata aagcgagccg tcggtgatgg cgagaacgcc cttggtcttg
     3241 ccgtggaagg agttttccgc atagacgatc ttcggacgtt tggcgccggc ggcgcgctcg
     3301 gcaagcttga ccgccgcctc catcgcctcc gatccggacg agccgagaaa caccatgtcc
     3361 aggtcgccgg gcgaacaggc agcgatatta tgcgccagcg ccgccgcata ttgcgacatg
     3421 aaggcgatgg caatttcctg gcgcttctct tcctgaaagc tctttcgcgc ctcaagaatg
     3481 cgcggatggt tgtggccgaa ggccagcgaa ccgaaaccgc cgaagaaatc gaggatcttg
     3541 cggccgttct ggtcgatata gaacatgccc tccgcacgct cgatcttgat cttgtggaag
     3601 cccagcagct tcatgaaatg cagctggccg ggattgatgt gcgccttgaa caggtccgtc
     3661 atgcgcgcca catccatcgc cttcgcctgc tcgacgccga tgaggttcgg cttgggcgtc
     3721 gttgccggcg aaagcgcggg cgcgttcacc atggtgcgtg ctgcggcctg tttgggtctg
     3781 gtcataacgg tcatcttgct ctcctgttgg cgcggtcacg gccgatagcc gccggaccgc
     3841 ccgcgaaatt gaacttactc cgccggcacg tggctggcgg cgacggcatg gccgtccttc
     3901 ttggcgcggt attcgctgta ggccgcgatc agcatgtcct cgtcgcggta tttcggcgcc
     3961 cagccgagct gccgctccgc cttggacacg tcgagcacgc attcctcgtc ggcgatcaga
     4021 tattgctccg ggtccatgat cggcatgttc atggcgtcga gcaggtcgag cgtgcgcttg
     4081 accgcccagc cgggcgtcgg gaccagcaat gacttcgacc ccgcatgctt gatgagatcg
     4141 cccagcagcc gcttgaccgg cggcgggttg agcgagccaa ggttataggc ctcgttcggt
     4201 acgccggcct tccacgccgc gcggcaggct tcggcgcaat cgaacaccga aatgaactga
     4261 taggggttct tgcccgaacc gatcatcggc accggcatgt tccagtcgat cagcttgaac
     4321 agcttttcca ggatgcccaa ccgtccggga ccgatgatga ggcgtggccg aaacaacgaa
     4381 atcgccatcc cgcgcttgcg ccattcggcg gccagttcct cggtcttctg cttcgaccag
     4441 ccatattcgc caagcggcgc gaccggatga tcttccgtca tcggataggt gacggtatgg
     4501 ccgtagatca tgtccgtggt gaaatggacg agctttggcg ctcccgcttt gtccatcgcc
     4561 tggatgatgt gttcggtgcc gtggaaattc accggaaaga agaagtcgtg acgcttggac
     4621 cgcacctgga tcggcgacag catcttggcc gacaggttgt agaccatgtc gtcggccttc
     4681 agcccgacgc cggcgactga agcggggtcc gtgacatcgc aatggatgtg gcgaaccgaa
     4741 cgatagtgcg aaagctcgct cttgacgata tcggcgacga tgacttcctc gccgtcggca
     4801 accagtttgg gcgcaaggtg gcggccgaca aagccgtcac cgccgaaaat cacatgtctc
     4861 atcgtacgat ctcgctggtg atgatcttgt cggtggaaag ggacgccgtc tcgtcgtcgt
     4921 gccccctgcc gctctgcgcg atcaggaccg tgccgacgca gatgcaggcg atgcccgcga
     4981 tgcgccagcc gttcaaatcc tcgcggaaaa ggaaatatgc aaataccgcg accgcgacat
     5041 aggcgaggct caggaacgga taggcaaacg aaagctcgac cttcgacaac acgtagaggt
     5101 gcgacgccat tgagatgacg aaggtgcata gcccaagaaa gacccatggg ctgaacacga
     5161 tctgcaatat cttgacgacc gggttcgcgc cctcgaagga aatcgggtta agtgagatca
     5221 tgccttgctt cagcattagt tgcgctgccg cattggtcat gaccgtaaat agtatgaagg
     5281 caatatattt catgattttc cgctcaactc tcgaacgatc ctattgcgca tccagaaaca
     5341 ttcgatgaat ccgaaggcaa gatttgcaac tgatcggctt cacggtgttt catcgtgaat
     5401 aatgtctgac cgtcttcatt ccgccgcctc gacgaggccg gatgaactgc ccgccaaacg
     5461 cttagccgtt cgcacccaga agctcgacat gaaagccgga tcgcgcaccg cctccaaccg
     5521 ctgccaggct ggaaaggacg ccgcaaaggt ttccggcccc atgcgcgcat ccttgtatgg
     5581 gttcaccgcg ccgccggcgt ccaccgttat gcgatccaat tcttcaagca acttgagcgt
     5641 tgcttcgccc cggttcggaa aatccagcgt cagcgtatag ccgggccttg gaaacgatag
     5701 cagcgccggc gaagcgatcg aaccgaagcg cttcagcaca gtcaggaaag agccctgccc
     5761 ggcgcgcttt gcggcgcgaa gaagcgccgg cacggcctca cgcgcgacat tttccggtac
     5821 cacgctctgg tgctggaaaa gccctcgcgg tccatagagc cggttccagt cgcgaacgcc
     5881 gtccagcggg aagaaaaagc cttgatagct ggcatggcgg ggctgtgtat ttcgtccgtt
     5941 cttccagcga taggcagcgt tgaacgcggt cagaaacggc cggttgagaa cgttgagcgg
     6001 cggctggaac ggaacggcaa gacgcccgcc cggacggcag gcgatgtgcg agccatgttc
     6061 ggcgtgattg ccggcaagca tgaggccacg cccgaactgt tgtccgccgg acagctgatc
     6121 gatccaggca acggcatatt cgtttgcctc gtccgcagcc attgcgagat cgaagaattc
     6181 gccgatttcc cggaatggcc tgacggtttc ggtgatatcc agggacggca cccgcatcaa
     6241 ccggatcgac gccgacaaga tgaggccggt caaccccatg ccgccgatgg tcgcggcaaa
     6301 caggtgcggg ttccggctcg ccgagcagcg atattcgcgg ccatcggagc gcagcagcgt
     6361 aaaaccctcg acatggcatc cgaacgtacc gcgccgatgg tggttcttgc catgaatgtc
     6421 gttggcgatc gcgccgccca gcgtcacgaa ttgcgtgccc ggaacaacag ccggaaagaa
     6481 gccgtgcggc gcggcatagg caatgatgtc ggacaaaagc acgcccgctt ccgcctcgat
     6541 catgccggcc tcggcatcga acgaaatgat gcggtcgagc gatcgcatat cgacgagcgc
     6601 tccatcccca ttcaggcagg aatcaccgta ggagcggcca ttaccatagg ccagcaacga
     6661 agcttcgccc gccccgcccc cgcgcaacaa tgcgatggcc tcgtccgccg gaagtgcatt
     6721 ggcggctgag ggcgtcgcgc gaccgaaact ggaataggcc gccctcacct caccagcctc
     6781 cgatcaccag cagcaccgcg ccaaggccga tgacgatctg gctgcgccaa tcgctgatga
     6841 tgaagacaac cgggtcttca tgcatttcgc cgcgccggcc aagaatccac acccgcatgg
     6901 tcagatagag cacgatcggc gccagcggcc acaccatcca cggattggga tagagttcgc
     6961 gcacggcgtc actgtccatg taaagcgcca gcaccagcgc ggaagaaaag gctgaagcca
     7021 tgccggcttg cgcgacgact tcacggtcct cgctgcgata gccacggccg gcaatgcgtt
     7081 ctccgggcgt caggctgggc aaacccagtt cgacataccg cttcaccagc gccagcgaca
     7141 ggaagaagaa catcgaaaac gccaacagcc agaacgatac gccgaccccg gtggccgccg
     7201 cgcccgcgat gacccgtacc gtgtagaggc cggccagcgt cagcacatca attagaagca
     7261 tgcgcttcag cgtcagcgaa taggcggtcg tggtcacgat ataggcggcc agcacgccca
     7321 tgaacggcca tgacaccagc aagcccgcgc caatgccgat ggcaagcaga acacagatgg
     7381 acgcaagccc gaacggaatg gacaacacgc cgctggcgaa tgggcggcgg cacttggttg
     7441 gatgcttgcg gtcgagcgcg aggtcgaaga agtcgttgac gatgtagatg gccgacgcca
     7501 cggcgctgaa tgaaatgaac gcgaacacgc attgcacgat catggcggcg ttcagatatt
     7561 catggctgag caccatgggc accgcgatca gtgtgttttt cgcccattgg tggacgcgaa
     7621 gcatcttcag gatcgatttc aacgaacgcc tgggcgccgg catgatttcg ctaccgtgtg
     7681 cggattgcca cagggcggcc ttgcgatcgg gcgcgaccac gatcgccgcg cgcgccgcat
     7741 cgaacacggc gacgtcgtgc cggctgttgc cggcatagtc gaaaccgcca tcgccatagg
     7801 cggcgaccag cgcagcccgc ttcttggccg aggtcaggtt ttcgccgccc tcggttgcca
     7861 gtaccgcatc gaagacgcca agatgggcgg caatcgcctc cgcgaatttc cgcggcgttc
     7921 ccgttgcaag cacgagcttg cgtccatcgg cgtgatcggc acgcaggcgc gccagcaaat
     7981 cctggcgata aggtagggct tcaggatcga tatcgacgcg gtcggcgatg gcctgtttca
     8041 gctttgccgg cccgccggca agccaaaacg gcaccaggaa cagataaagc gggttcttcc
     8101 tgagaagcgc aaacaggccc tcccaaagca gatcggtggc gatcagcgtg ccgtccagat
     8161 cgacggctag cggtattgct gtcctgtccg cccgagcgtc catcccgttc ctgcccttgc
     8221 ctcgtcacgc cacctgcggg cgtccctgaa aaagctatag cggtggacag gctaccgaac
     8281 gttaaaaggc ggcgcttgaa aagagatgac tggcggaaat tccattgcca tcgttaaatt
     8341 gccattactg caaatgttaa aaggcccgga atgcaaaaag gcagggcaat agtcccggcc
     8401 ctttcgaaga atacacaagt ttacttgata tgggcgacgc cgcccgaaat ctctacggtt
     8461 tccgatcccg tcagcggcgt gaggtccccg ttcggcaagg tcacgaagca acccttcggg
     8521 cagaactccg ccgtttcgcc gctcgccagg gcaagctccg atttgctgcc gccttcggtt
     8581 acgatcagcg tgcgcgtttc gccatcattg ttgacagcgc tcgcggcgta ggccgacgac
     8641 gccccggcaa ccagcatgcc ggcaaggaca accgatttca acatggcact ttccggtgct
     8701 tccaccgcct ctgttgcaag gcattttcgc cctttgcatc cagacttata gacgatgaaa
     8761 gctgaaccgc aactgaatgc cgcattcatg ccacaattgc ccttggccga gccggcaatc
     8821 gcccgtggcc gaatttgtca gccgggatcc ggcttgcggc tcttgcgccc gcggccggaa
     8881 ttgcgcttgg cggcggcatc ggcttttgcc tgctgttgtt cggcaaactc cacttccgtc
     8941 ttgtcgtcgt cttccggcca tggatcgtgt ttttccacag cgacaaccgc tctggccgtc
     9001 gatggaattt cgatgtgctc cttgtcgaac atctccagaa tggaaaagcg gatatcgttc
     9061 tgcaccgcgc tgccatcggt gatgtcggcc aggaaaaccc tgacctcgaa ttccagcgcc
     9121 gccggcccga aattggcgaa caaaacgaac ggctccgggt tcttcagcac caatggatgc
     9181 gcgcgcgcta tttcaagcat gatctcatgc gcccgcctga aatccatgcc ataggcgacg
     9241 ccgaccttga tctcgacgcg gccaagcttg ttgcggtgcg tccagttgcc aaccgcatta
     9301 ttgatcagat tggaattggg caggatgacc gactggcgct ggaacgtctc gatctcggta
     9361 gcccgcacgc tgatcttctt gacggtaccg gacacatcgc ccgccacgat ccagtcgccc
     9421 accttgaacg gccgctcggc cagaaggata aggccggaga cgaaattcga aaccacattc
     9481 tgcaagccaa agccgatacc gagcgaaagc gcgccggcca ccagagcgag actggaaagg
     9541 tcgatgcccg ccgccgaaat gccgacgagc gcggcgacgg ccacgccggc atagcccacc
     9601 gccaggcgta tcgaattgcg cacgcccgta tcgaccttgc cgcgcgccat gaccgaaccg
     9661 tcgagccagc cctgaaacca gcgggtcagg aaatagccga tgacgaaaac cacgatgccg
     9721 ctgagaatgc cggtaacgga gatcgtcgcg gaaccgatgg tcagcccggt agcaagcttg
     9781 tagccccagg cctggatgtc gccgagctga aacccccaca tcagcaggat caacggcagg
     9841 aagatcacta cgatcatcag attgagggcg acgctgacgg caaggccaag ctggtcgagc
     9901 gtcgcttcct ccacattgcc cttttccgtc agccagcgcc cgatggatga actgccgaag
     9961 ccgccttccg cgccgatggc gcgtgccgac aggaagccga tataggccgt cagcaggatc
    10021 gtgccggtca ccaccacttg cagcgacacg aagatcgcca ggccgatata gccgagcagc
    10081 gccgcgccga tggtgaacgc gccaagcgca aaggcgctgt agcgcagcca tgccggccac
    10141 ggcttccaga cgccatccgt cgtgcagaac gggcgcacca gcgccatcag gatcaggatg
    10201 acgccgaaga cgatggtagc caggaagctg cgcgcgatcg tcagcgacag cggcgaattc
    10261 atctggtcgt tgacgatgga aaggaaggta ttgccgccga tgaccagcgc catggccgtc
    10321 gccagccgca caagccagcg gccgggcgtc gattccaccg gtatcagccg ccagttgggc
    10381 aggcgaggcg aaagggcggc gttagccagc cggttgacgc aaaagataac cgcgatgagg
    10441 gcggcgaacg cattgaggaa gacgccgata tctccgcgca ggacattgta ataattgaaa
    10501 aagaagatgg tcgagaccag aaataccaca agggcggctg tcggcagcag cgtcgaccag
    10561 aacgtcacgg aaagccggct gagataggac ggatcgtcca cagtcgggtc agccacgaac
    10621 atgcggccga acacgcggcg tccgcccacg atcagcacgg ctgcgaggcc gagcgccatg
    10681 aacgtcgccg caagcatcgc ctggaacttg aacttgaggg caaaggagaa ccacgacgag
    10741 aaggcgccgt aaaaccggac catttccgcc ttcgcgtcgg cgagcacctt gccgctgagc
    10801 gtatcggcca gcgtgtagcg cttggtcagc aggttgcgga acaggtcgct gcggatggcg
    10861 ccgatctgtt cgatcagccc gctgatgcga attgacaggg tctgcgccgt cgcgatcacc
    10921 ccgttgatct gggctttccc tgcaatcagc gcatcgcgct ccgccgtaac gatgtccggt
    10981 tccgccggct ggccttcggc aggccccggg ccaagctgtt caagccgcgc atttatgtcg
    11041 gccgtgcttg aacgaaacgc cagcgcgctt tccagagagc gctgcgacag gccttccagc
    11101 ttcagccgga tttcgaccag ggcggcgtcg tcctggccgt tctgctgcat ctgcttttcg
    11161 agatcgtcgg tcttcgcggt cagttgctgg atcacctgct gctgcgcggc gacaaggccg
    11221 ttggcggttt cactgacgct ttgcgcggcg gctccggatg ggagcaacgc caaaagcgca
    11281 agaacgacgg cgaaacggta aaaacgctgg agcatgattg acgagcgact cgtggaaagc
    11341 catgacggct cgattgcatc attgataaag gcggcggtat caaggggcaa gccacgcccg
    11401 ttcggcaagt cggccaatcg aaaagccatg gcgcaatagt ggctttcagg ctatcgccgg
    11461 ctctatagtc tgcgcagtcg cccataatgg aagaaaaaag aatggcggaa tattcagcgc
    11521 tttcactcct gaaaaatgca ttcacaggca ataaggactg gaagcccgcc tggcgcaagc
    11581 cgacgccgaa gccatcttat gacgtcatcg tcatcggcgg tggcggccac ggcctgtcca
    11641 ccgcctacta tctggccaag gaacacggca tcaccaatgt cgcggtgctg gaaaaaggct
    11701 ggctggggtc gggcaatgtc ggccgcaaca ccactgcggt ccgttccaac tatctgctgc
    11761 cgcccaatac gcgcttctac gagcattcga tgaagctgtg ggaggggctt tcccacgacc
    11821 tcaactataa cgtcatgttc tcccagcgcg gctgcctgaa ccttgcgcac acgccggccc
    11881 aactcgatga ctatgccagg cgcggcaacg ccatgcgcca tcagggcgtc gatgccgaac
    11941 ttatgacgcc tgcgcaaatc aagcgcctta ttccggcgct ggacgtgtcg gatgcggcgc
    12001 gctttcccgt ctatggcggc ctgatgcagc cgcgagccgg cacggctcgc cacgacgcgg
    12061 tcgcctgggg ctatgcgcgc ggcgccgacc ggcgcggcgt cgatatcgtc gagaactgcg
    12121 aagtcaccgg cttcctgcgc gacggcgacc gcattgtcgg cgtcacgacc acgcgcggcg
    12181 aaatccgcgc caggaaagtg gccgtggcgg tcgccggcag caccggccgc gtgatgcagc
    12241 ttgccggcat cgacaccatg cccatcgaga gccacgtcct ccaggccttc gtcacggaat
    12301 cgctgaaacc cttcatcgat acggttgtga ccttcggcat gggccatttc tatatgtcgc
    12361 agtccgacaa aggcggcctt gtctatggcg gcgacctcga tggctacaat tcctatgctc
    12421 agcgaggcag cctgcccgtt gtcgaggagg tgatgagcga gatgctggcg ctgttccccg
    12481 gccttgccgg cgtgcggctg ctgcgctcat ggggcggcgt ttgcgatatg tcgatggacg
    12541 gctctcccat catcaccacc ggccccctgc ccggcatgta tctgaactgc ggctggtgct
    12601 atggcggctt caaggcgacg ccggcttcgg gctggtgctt tgcccacacc atcgccaagg
    12661 atgcgccaca cgaattgaac gccgccttca cgctcgaccg ttttcatcgc ggcattctca
    12721 tcgacgagaa gggccagggc gcgacgccgc gcctccatta gggtagaaaa atgctgatca
    12781 cctgtcccta ctgcggtccg cgcgacgtgt ccgaattcac ctatcagggc gatggcaacc
    12841 gcgagcggcc tcaacccgcg tcgcagaacc tcgatgcctg gaacgagtat gtctatgagc
    12901 gtctgaaccc ggccggcgac cataatgaga tatggcagca ttccggcggc tgccgcaccc
    12961 atgtccgcgt cgtgcgcaac acgctgacgc atgtgatttc aagcgccatt ccggcccgcg
    13021 ccgaggcaag gcacaaggcc gccagccgcc gtggagcaaa gtcatgagtc cgcgccgcgc
    13081 ccttgccggt ggcagcatcg accggctgaa gacgatccgc ttcaccttcg acggcacggc
    13141 tttcacaggc catgccggcg atacgcttgc ctcggccatg cttgcaaacg gcgtttcgct
    13201 gttcgggcgc tcgttcaaat accaccgccc gcgcggcgtg ctgaccgccg gcgtcgatga
    13261 accgaacgcc ttggtgacga tactgcgcgg cgaggtgcgc gagccgaaca ttccagccac
    13321 catgatcgag attttcgacg gcctcgccgt tgaaagccag aaccgcttcc cctcgctcgc
    13381 ctgggacgtg aacgcggtga accagcttgg cggcaagctg ctttcggcgg gcttttacta
    13441 caagaccttc atggggccgg cgatcggccc gctgaaaggc accggtttct ggatgttctg
    13501 cgagaaattc
//
LOCUS       DASYCS010000240         5250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1508847,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000240 DASYCS010000000
VERSION     DASYCS010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 5250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5250
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1508847"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..982)
                     /locus_tag="VGM46_07700"
     CDS             complement(<1..982)
                     /locus_tag="VGM46_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEY4192516.1"
                     /translation="MDNQPQQQDAKLPIPRWEFIALCAALMALNSLAIDIMLPALQQI
                     GGSLGVENPNHRQYVITAYIVAFGIAQLGFGPISDRFGRRGPLLVGLAIYILAAFAAV
                     AAPSFETLLLCRFVQGIGAAATRVISVSVIRDSFGGRRMAEIMSLVFMVFMAIPVVAP
                     GIGQIIMIFATWHLIFAAMGVGALLITLWAFLRLPETLHAEFRRPFTVAAVTGGFRIV
                     LSNRTALSYTLAGMATFGAMFGFINSAQQIYIDIYHLGVWFPVIFAGIASMMAASNFA
                     NARLVGRFGMRRLSQGALLGFIAVSAVWLVVSLAGPVPFPIFITLFALDML"
     gene            complement(1097..1432)
                     /gene="grxD"
                     /locus_tag="VGM46_07705"
     CDS             complement(1097..1432)
                     /gene="grxD"
                     /locus_tag="VGM46_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018095088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEY4192517.1"
                     /translation="MSGINDYIDNEVKSSDVVLFMKGTPGFPQCGFSGQVVQILDHVG
                     ADYKGVNVLDSAELRQGIKEYSNWPTIPQLYVKGEFVGGCDIIREMFQAGELQSFFVE
                     KGVSVKGAA"
     gene            1754..2932
                     /locus_tag="VGM46_07710"
     CDS             1754..2932
                     /locus_tag="VGM46_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015340048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192518.1"
                     /translation="MPHIAKRIVLHFPGFEPLDAEAHRSRYARSAMQSGQVWNYKVET
                     GPLGGNPLAPAFTVDAAGAGWSTQSHVHILDHNGLIGALGARSPLARLAAGYRSAARV
                     VWHGGMFGFFRHAWRFALFFLFPFLLVALGFAALSFAGTLPVLFGLSAWHLVWSLPVA
                     WLLFFKLFLPVAERAYTLHLCDDWELAVALACLDDDAANRRLADCVIAARAALQEPAD
                     EYLISSHSMGSSVATHVIGALLQEDPEIFTGKKVVFATLGGAVLQCSLLKPASVLRSR
                     VGAIARSANVFWLDVQCLTDVINFYRVRVVKAAGFPDLPQARILFIRFKHMLTPERYR
                     RLKKDFLRMHRQYVLGPDRRAAFDFTLMTAGPLSARDFADFTADRLAPLAPADAGLAA
                     "
     gene            complement(2937..3170)
                     /locus_tag="VGM46_07715"
     CDS             complement(2937..3170)
                     /locus_tag="VGM46_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006466620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEY4192519.1"
                     /translation="MAMDAHDIEKMIQEGIPDARITIRDLAGDGEHYAAEVVAESFRG
                     KSRVQQHQMVYEALKGNMGGVLHALALQTSPPE"
     gene            complement(3186..>5250)
                     /gene="purL"
                     /locus_tag="VGM46_07720"
     CDS             complement(3186..>5250)
                     /gene="purL"
                     /locus_tag="VGM46_07720"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEY4192520.1"
                     /translation="SSKKWLRTLPTTGAVVIQGPGENAGVVDIGDGDCVVFKMESHNH
                     PSYIEPYQGAATGVGGILRDVFTMGARPIAAMNALRFGAPDHPKTKHLVAGVVSGVGG
                     YGNSFGVPTVGGEVNFDARYNGNILVNAFAAGLAKTDAIFLSEAKGVGLPVVYLGAKT
                     GRDGVGGATMASAEFDDKIDEKRPTVQVGDPFTEKCLLEASLELMASGAVIAIQDMGA
                     AGLTCSAVEMGAKGDLGILLDLDTVPVREERMSAYEMMLSESQERMLMVLRPEKEAQA
                     KAIFAKWGLDFAIVGKTTDDLRFRVMHQGTEVADLPIKDLGDKAPEYDRPWVEPKKPA
                     ALAANDIPQADIAEALLKMLNGPDLSSRRWVWEQYDTLIQGNSLQLPGGDAGVVRVEG
                     HATKALAFSSDVTPRYCEADPFEGGKQAVAECWRNMTATGALPLAATDNLNFGNPERP
                     EIMGQLVAAIKGIGEACRALDFPIVSGNVSLYNETNGQGILPTPTIGGVGLIDDWAKM
                     ARVAFAAEGQTILLISASEQWGTHLGQSIYLRDIHGRSDGPPPHVDLAHEKKAGDFVR
                     GLIRNGEASAVHDLSDGGLAVALAEMAMASGIGASIEQPQGGDAIAPFFGEDQGRYLV
                     TLPFPADDARSGAVQERAKALGLFASPIGTTGGAELKLGNARAITVDELTTAHESWFP
                     RFMGN"
BASE COUNT          928 a         1648 c         1663 g         1011 t
ORIGIN      
        1 aaagcatgtc cagcgcaaac agggtgatga agatcgggaa cggcaccggc cccgccaggg
       61 aaaccacgag ccacaccgca ctgaccgcga tgaagcccag cagcgcacct tgcgaaaggc
      121 ggcgcatgcc gaagcggccg accaggcgcg cattggcgaa attggacgcc gccatcatgc
      181 tggcgatgcc ggcgaagatg accggaaacc acacgcccag atgatagatg tcgatataga
      241 tctgctgcgc ggaattgatg aagccgaaca tcgcgccgaa ggttgccatg ccggccagcg
      301 tgtaggaaag agccgtccgg ttggaaagga cgatgcggaa accgccggtc accgccgcaa
      361 cggtgaacgg acggcgaaac tccgcgtgaa gggtctcggg aaggcgcagg aacgcccata
      421 gtgtgatgag gagcgcgccg acgcccatgg cggcgaaaat caggtgccag gtcgcaaaga
      481 tcatgatgat ctggccgatg cccggagcca ccaccgggat cgccatgaac accatgaaca
      541 ccagcgacat gatttccgcc atgcgccgtc cgccgaacga atcgcggatg acggagacgg
      601 aaatgacgcg cgtggccgcc gcaccgatgc cctggacgaa ccggcacagc agcagcgttt
      661 caaagctggg cgccgccacc gccgcaaagg cggccagaat atagatggca agcccgacca
      721 gaagcgggcc gcgacggccg aagcggtcgg agatcggccc gaagccaagc tgcgcgatgc
      781 cgaaggcgac gatataggcc gtgatgacat attgccggtg gttggggttc tcgacgccaa
      841 gcgagccgcc gatctgctgt agggccggca gcatgatgtc gatcgccagc gagttcagcg
      901 ccatcagcgc cgcgcatagc gcgatgaact cccaacgcgg aattggcagt ttcgcgtctt
      961 gctgctgtgg ctgattgtcc atgacggttc cgatcttcaa acaaaaatgc gccggctgac
     1021 cggcgcattt cttttgtctt ttccccaaac cgggggtgaa gtgtctaccg ggatgggggc
     1081 agcccgatga taatggtcag gcggcgcctt tgacgctgac gcccttttcg acgaaaaacg
     1141 actgcaattc gcccgcctgg aacatctcgc ggatgatgtc gcagccgccg acgaactcgc
     1201 ccttgacgta gagctgcgga atggtcggcc agttggaata ttccttgatg ccctggcgca
     1261 gttcagccga atcgagaacg ttgacgccct tgtagtcggc gccgacatgg tcgagaatct
     1321 gcaccacttg gccggaaaac ccgcactgcg ggaagccggg cgtgcccttc atgaaaagca
     1381 ccacgtcgct gcttttcact tcattgtcga tgtaatcgtt gataccgctc atgatttatc
     1441 ctttcacgcc ggtcgaagcc aggctcgaag catgcccttc aaataacctt ccagggcgtt
     1501 cgaaacaagt cgtttgccgg tccggcctcg aaatggctta aggtttaccg gttcgctggt
     1561 ccaagttcga cgatatcggc gaggtcggac ttcgcgcaaa ggcaggcata aagcagcccg
     1621 cctcgtttac aatccgggaa gcggtgaagt agtaagcgcc aggaagcagg gtcctccgcc
     1681 gtgatggcct gcaatgcccg agccgttcaa ccgttcccga gcgcgctcgg tctcacttca
     1741 aggccactaa cccatgcctc acatcgccaa gaggattgtc ctgcattttc cgggtttcga
     1801 gccgctggat gccgaggcgc atcgcagccg ctatgcccgc tcggccatgc agagcggaca
     1861 ggtgtggaac tataaggtcg agaccggacc gctcggtggc aatccccttg cgccggcttt
     1921 caccgtcgat gctgcggggg ccggctggtc gacgcaaagc catgtccata ttctcgacca
     1981 caacgggttg atcggcgcat tgggcgcgag atcgccgctg gcccgcctgg cggccggtta
     2041 tcgaagcgcg gcgcgggttg tctggcatgg cggcatgttc ggctttttcc gccacgcctg
     2101 gcgcttcgcg ctgttcttcc tgtttccatt cctgttggtg gcgcttggtt ttgcggcgct
     2161 gtcgtttgca ggcacgctgc cggtgctgtt cggcctgtcg gcgtggcacc tcgtctggag
     2221 cctgccggtg gcatggctgt tgtttttcaa gctgttcctg cccgtcgccg agcgggccta
     2281 tacgctgcat ttgtgcgatg actgggagct tgcggttgcg ctggcctgcc tggacgacga
     2341 tgcggccaat cgcaggctgg ccgattgcgt cattgccgcg cgcgccgccc ttcaggagcc
     2401 ggcggatgaa tatcttatca gctcgcacag catgggatcg tccgtcgcca cccatgtgat
     2461 cggcgcgctt ctccaggagg accccgaaat cttcacgggc aagaaggtgg tcttcgccac
     2521 gcttggcggc gcggtgcttc aatgttcgct gctgaagccg gccagcgtgc tgcgcagccg
     2581 cgtcggcgcc atcgcgcgct cagccaatgt tttctggctg gacgtgcagt gcctgaccga
     2641 tgtcatcaac ttctataggg tgagggtggt gaaggctgcc ggctttcccg acctgccgca
     2701 ggcccgcatc ctgttcatcc gcttcaagca tatgctgacg ccggaacgct atcgccggct
     2761 caagaaggat tttctgcgca tgcaccggca atatgtgctg gggccggacc ggcgcgcggc
     2821 ctttgatttc accttgatga cggccgggcc gctatcggcc agggatttcg ccgatttcac
     2881 ggccgacagg ctggccccgc ttgcgcccgc agatgctggc ctggccgcat agcgccttat
     2941 tcgggtgggc tggtctgtag cgccagcgcg tgcagaacgc cgcccatatt gcctttcagc
     3001 gcctcgtaga ccatctggtg ctgctggacg cggcttttgc cgcgaaagct ttcggccacc
     3061 acttccgcgg cataatgctc gccgtcgccg gccagatcgc ggatggttat cctggcgtcc
     3121 ggaatgcctt cctggatcat tttttcgatg tcgtgcgcgt ccatcgccat gtttggtttc
     3181 cttgcttagt ttcccatgaa gcgagggaac caggattcgt gagccgtggt caactcatcg
     3241 acggttatcg cacgggcatt gccgagtttc aattctgcgc cccccgtcgt gccgatgggc
     3301 gatgcgaaaa ggccgagcgc cttggcgcgc tcctgcacgg cgccggagcg ggcatcgtcg
     3361 gccgggaagg gcagcgtcac cagatagcgg ccctgatctt ctccgaagaa cggtgcaatg
     3421 gcatcgccgc cctgaggctg ctcgatggaa gcgccgatgc cggaagccat cgccatttcg
     3481 gcaagcgcca ccgccagtcc gccgtccgac aggtcgtgga ccgcacttgc ttcgccgttg
     3541 cggataaggc cgcgcacgaa gtcgccggcc ttcttttcat gggccaggtc gacatgcggc
     3601 ggcgggccat cgctgcggcc atggatatcg cgcaggtaga tcgactggcc aagatgcgtg
     3661 ccccattgct cgctggcgct gatgagcagg atagtctggc cctcggcggc gaaagccacg
     3721 cgcgccattt tcgcccagtc gtcgatcagg ccgacgccgc cgatggtcgg tgtcggcaag
     3781 atgccctggc cgttggtttc attgtagagc gagacattgc ccgacacgat cgggaagtcg
     3841 agcgcgcggc aggcctcgcc gattcctttg atcgcggcga ccagctggcc catgatttcc
     3901 ggccgctcag gattgccgaa attgaggttg tcggtggcgg ccaggggcag ggcgccggtc
     3961 gccgtcatgt tgcgccagca ctcggccacc gcctgcttgc caccctcgaa cgggtcggcc
     4021 tcgcagtagc gcggcgtaac gtcggaggaa aatgccagcg ccttggtcgc gtggccttcc
     4081 acccgcacca caccggcgtc gccgccgggc agttgcagcg aattgccctg gatcagcgtg
     4141 tcgtattgtt cccacaccca gcggcgcgac gacaagtccg ggccgttgag cattttcagg
     4201 agcgcttcgg caatgtcggc ctgcggaatg tcgttggcgg caagcgccgc cggcttcttc
     4261 ggctcgaccc atggccggtc atattccggc gccttatcgc cgagatcctt gatcggcagg
     4321 tcggcgactt ccgtaccctg atgcatgacg cggaagcgca aatcgtcggt ggttttgccg
     4381 acaatggcga aatccagccc ccatttggcg aagattgcct tggcttgcgc ctctttttcg
     4441 gggcgcagca ccataagcat gcgttcctgg ctctcggaaa gcatcatttc ataggcgctc
     4501 atgcgctcct cgcgcaccgg cacggtgtcg aggtcgagca ggatgccgag gtcgcccttc
     4561 gcgcccattt ccaccgccga acaggtgagg ccggcggccc ccatgtcctg gatggcgatg
     4621 acggcgcccg acgccatcag ttcgaggctg gcttccagca ggcatttttc ggtaaacgga
     4681 tcgccgacct gaacggtcgg ccgcttctcg tcgatcttgt cgtcgaactc tgccgaagcc
     4741 atcgtcgcgc cgccgacgcc gtcgcggccg gtcttggcgc cgagatagac caccggcagg
     4801 ccgacgccct tggcttccga caggaagatg gcatcggtct tggccaatcc cgccgcaaag
     4861 gcgttgacca ggatgttgcc attgtagcgc gcgtcgaaat tgacctcgcc gcccaccgtc
     4921 ggcacgccga aggaattgcc gtagccgccg acgcctgaaa cgacgcccgc caccagatgc
     4981 ttcgtcttgg ggtgatcggg cgcgccgaag cgcaatgcgt tcatcgccgc gatcgggcgg
     5041 gcgcccatgg tgaacacgtc gcgcaggatg ccgccgacgc cggtcgcggc accctgatag
     5101 ggttcgatgt aggacgggtg gttgtggctc tccatcttga agactacgca atcgccgtcg
     5161 ccaatatcga ccacgccggc attttcgccg ggcccctgga tgacgaccgc gccggtggtc
     5221 ggcaaggtgc gcagccattt cttggatgat
//
LOCUS       DASYCS010000241         7480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2958293,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000241 DASYCS010000000
VERSION     DASYCS010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 7480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7480
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2958293"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="VGM46_07725"
     CDS             complement(<1..874)
                     /locus_tag="VGM46_07725"
                     /inference="COORDINATES: protein motif:HMM:NF022436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2793 domain-containing protein"
                     /protein_id="HEY4192521.1"
                     /translation="MDTSPNLTLPYIAPSQAQKHVTHNEAIRALDALVQLSAHDRDLA
                     SPPATPADGDRFIVAASGSGPWAGHDGEIAAFQDGGWAFFKPKPGWLAYVADEAALLV
                     WDGAAWANVVAGTAGITQLGINATADAVNRLVVKSDAALFDNDGADARVNINKAAVGQ
                     TASVILQDAYSGRCELGLAGDDDFRIKVSANGSTWREALRTEAASGEVIIDNMRVTRE
                     IAPNLLPDSGRFNGNAGNATFSGIAYSAPSYFAAVSGGSIASHAKFVHDNADFGGAGA
                     ALDPEVRALVEKIRP"
     gene            1098..2399
                     /locus_tag="VGM46_07730"
     CDS             1098..2399
                     /locus_tag="VGM46_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEY4192522.1"
                     /translation="MTINLQDAKIAVVGLGYVGLPLAVEFGKKRQVLGFDTKRKRIEE
                     LRAGEDATLEVSADDLASASGLVFSCEAADMADCGVFIVTVPTPIDDANRPDLSPLIR
                     ASESIGAVMPQGAVIIYESTVYPGATEEICVPALERASGKKFNVDFFCGYSPERINPA
                     DKQNTLPKIVKVTSGSTPEAADAIDRLYASIITAGTFKASSIKVAEACKVIENTQRDL
                     NISLMNELSIVFARIGIDTAEVLEAALTKWNFLNFRPGLVGGHCIGVDPYYLTHKAEQ
                     LGYSPQVILAGRRINENMGRYVARNTIKLMLKNGMDVPRCRVGVLGVTFKENCPDTRN
                     SRVIDLIRELQDHGVEVLAADPHADPEEVKAEYGIEITRLSPETPVDALVVAVGHNEY
                     REMTSAQLRALTRGDKPVLSDVKSLYDRHAAAKEGFQVFRL"
     gene            2396..3448
                     /locus_tag="VGM46_07735"
     CDS             2396..3448
                     /locus_tag="VGM46_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY4192523.1"
                     /translation="MTTNNSNSKVRFALVGAGRIAANHLKAMESHADRCELVSICDTD
                     EQVLAKIAAQTGAKGFSRLDQMLANTPADCVVLTTPSGLHASQAIAVAQSGRHVITEK
                     PMATRWKDGLAMVEACDKAGVKLFVVKQNRRNATLQLLREAVRQGRFGRIYSVSVNVF
                     WTRPQEYYDAAKWRGTWEFDGGAFMNQASHYIDLLQWIVGPIESVMAYTGTLARDIEA
                     EDTGVAALRWRSGAMGTLNVTMLTYPKNLEGSITILGETGTARIGGVAVNEIQEWSFA
                     DKRPEDDEIKNASYQTTSVYGFGHPLYYDNIIKTLRGEEAAETDGRQGLKSLELLVAM
                     YMSARDGRRVALPLEH"
     gene            3450..4040
                     /locus_tag="VGM46_07740"
     CDS             3450..4040
                     /locus_tag="VGM46_07740"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003113426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEY4192524.1"
                     /translation="MNYKAHPSAIVDDGAQIGDDTRIWHWVHVSAGARIGRGCSLGQN
                     VFIANRVTIGDNVKIQNNVSVYDNVTLEDDVFCGPSMVFTNVYNPRSAVSRKEEYRDT
                     TVRQGATLGANCTIVCGVTIGEYAFVGAGAVVSRDVAAYALMVGVPARRIGWMSRHGE
                     RLDLPASGSGEAACPVMGDLYRLQDDRVERIEAAPR"
     gene            4037..5164
                     /locus_tag="VGM46_07745"
     CDS             4037..5164
                     /locus_tag="VGM46_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEY4192525.1"
                     /translation="MNTQTDAQAPIQFIDLKAQYADLRGPVNARIQAVLDHGQYIMGP
                     EVAELEAALSTYTGAKHCITVSSGTEALLIAMMGLGIGPGDEVITTPFTFAATAEMIV
                     LLGARPVFVDVETATCNIDAGLIDAKITARTKAIMPVSLYGQAADMDEINEVAARHGV
                     AVIEDAAQSFGADYRGKKSCNLSTVGCTSFFPSKPLGCYGDGGAMFTNDDALAKAFRE
                     IRVHGQSKRYVHTRVGVGGRMDTLQCAIVLAKLERFDWEVGRRLELGARYNRLLDEAG
                     VKRVHQRQDRNSVFAQYTVLVENRTEIQESLQASGVPTAIHYPIPLNLQPAYAHLNVD
                     GQTVNSEWVAGRVMSLPMSAYLTEDQQDRIVETLKAAVWRP"
     gene            5152..6522
                     /locus_tag="VGM46_07750"
     CDS             5152..6522
                     /locus_tag="VGM46_07750"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEY4192526.1"
                     /translation="MAALIARLKGLASNDFIRSVGVLVGGTAAAQVLMAVSLPIATRL
                     YSPTDFSLLALFTAIVSIISVSACLRFDIAVPIPESDEEAANVLALAVGFAVLVSAVL
                     ALLAIFAPGPIVRLLNRPDFQPYLWLVPLTVLLSGLFSTLQYWFVRRKSFRAIATTKM
                     GQATGASATLVGLGFAHPTPLGLLLAYAINSGAGFIGLSYRFLRTDVALLRQVSWQRM
                     KHAFAANSRFPKYSTLEAACNSASIYLPIILISSLAAGPEAGYVIVAIQVMQAPMSLI
                     GSAIAQVYLSRAPEEFRADNLGAFTASIFSGLLKTGVGPLIFGGIVAPEVFSLVFGQG
                     WRPAGELVLWMTPWFVAQFLSSPLSMALHVTGNQRLALCLQIFGLIVRAGSIYAAYLV
                     FPKYLSETYAISGFIFYSVYNYQIFRITGTGLYSLFGSLIKNSIFLLFWISMAIILKV
                     IFYMYW"
     gene            complement(6559..>7480)
                     /locus_tag="VGM46_07755"
     CDS             complement(6559..>7480)
                     /locus_tag="VGM46_07755"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEY4192527.1"
                     /translation="PYDRNISAFESTKLLICWISLYGAGYFLRPSERLAHLLTASLVV
                     MAVLIPFVMATDPLSPDQALRWRFQGQVASYAWFANAIAFTAIFALALETTVVLRVVV
                     VVLGALALAYTGARAELFGYMFVVMVWLAASSQRRYILPAAAALLMFFIVALFVVGYF
                     HPQLLTRYTNIGSSASLNERWQIMIGGIKDIMANPIFGDYGGYIRSQKDFGSYIHNAL
                     SMWHQFGIVPFILYILMTTGCLIAAVRRRIAGRDFDARWWSLLLVSAFCCLLIVVAKS
                     VFWPLPALAWGLQSQLSKDREQRSHKDNYG"
BASE COUNT         1542 a         2293 c         2217 g         1428 t
ORIGIN      
        1 tgggtctgat cttctcgacc agcgccctga cttccggatc gagcgctgcg cccgcgccgc
       61 cgaaatcggc gttgtcatgc acgaatttgg catggctggc gatcgacccg cccgacaccg
      121 ccgcaaaata ggaaggggcg gagtaagcaa tgccgctgaa ggtggcatta ccggcattgc
      181 cgttgaacct gccgctgtcg ggcagcagat tcggcgcgat ctcgcgcgtc accctcatat
      241 tgtcgatgat gacctcgccg ctggccgctt ccgtgcgaag cgcctcgcgc catgtcgaac
      301 catttgcgct gaccttgatg cggaaatcgt catcgccggc caatccgagt tcgcatcggc
      361 cggaataggc gtcctggaga atgacgctgg cggtttgccc gaccgccgcc ttgttgatgt
      421 tgacgcgcgc atcggcgccg tcattgtcga atagtgctgc atcgcttttc accaccagcc
      481 ggttgaccgc atcggcggtt gcgttgatgc caagctgcgt gatgccggcg gttcccgcca
      541 cgacgtttgc ccatgccgcc ccgtcccaga ccagcagcgc cgcctcgtcg gctacatagg
      601 cgagccagcc cggttttggc ttgaagaacg cccagccgcc atcctggaaa gccgcgatct
      661 cgccgtcatg tcccgcccac ggaccgctgc cggatgctgc gacgataaaa cggtcgccat
      721 cggcgggcgt ggcgggcggc gaggcaagat cgcggtcatg cgcggacagt tggaccagcg
      781 catcgagcgc gcgtatcgcc tcattgtgcg ttacgtgctt ctgggcctgg ctgggcgcga
      841 tgtagggaag tgtcagattt ggtgatgtgt ccatgaaaat tcctttcgcg ttgaccgccc
      901 agggtacgcc ctgtcaggga gggtgtgggt ggatcggcgg cgtcgaaggg cttgagaaag
      961 gcgaccagtt cgcgtatggc ctgcaaaatc tgggaggcct tcccgcttca acagccttgc
     1021 agcccgagat gcggccagcc tgaaacgccg tatccacacg gctcggttca cactaggatg
     1081 aaagccggtt tccaagcatg acaatcaatc tgcaagacgc taaaatcgcg gtggtcgggc
     1141 ttggttatgt cggactgcct cttgccgtgg aattcggaaa gaagcggcag gttctcggtt
     1201 tcgataccaa gcgcaagcgc atcgaagaac tccgcgccgg cgaagatgcg accctggaag
     1261 tttcggctga cgatctggcc agcgccagtg ggcttgtgtt ttcctgcgag gccgccgaca
     1321 tggccgactg cggcgtcttt atcgtgaccg tgcccacccc catcgacgac gccaaccggc
     1381 ccgacctgtc gccgctgatc cgcgcttccg aatccatcgg cgcggtgatg ccgcaagggg
     1441 ccgtgatcat ctacgaatcc accgtctatc ccggcgctac cgaggaaatc tgcgtgccgg
     1501 cgctggagcg ggcttccggc aagaaattca acgtcgattt cttctgcggc tacagccccg
     1561 aacgcatcaa tcccgccgac aagcagaaca cgctgccaaa gatcgtcaag gtcacgagcg
     1621 gatcgacgcc cgaagccgcc gacgccatcg accgcctcta tgccagcatc atcaccgcgg
     1681 gaacgttcaa ggcgagcagc atcaaggttg ccgaggcctg caaggtcatt gaaaacacgc
     1741 agcgcgacct gaacatctcg ctgatgaacg agctgtcgat cgtgttcgcc cgtatcggca
     1801 tcgacacggc ggaggtgctg gaagcggcgc tgacaaagtg gaatttcctg aatttccggc
     1861 caggcctggt cggcggccac tgcatcggcg tcgatcctta ttatctgacg cacaaggccg
     1921 aacagctcgg ctacagcccg caggtcattc ttgccggccg ccggatcaac gaaaacatgg
     1981 ggcgctacgt cgcgcgcaac accatcaagc tgatgctgaa gaacggcatg gacgtgccgc
     2041 gatgccgcgt cggcgtcctg ggcgtcacgt tcaaggaaaa ttgcccggat acccgcaatt
     2101 cgcgtgtgat cgacctcatc cgtgagttgc aggaccacgg cgtggaggtg ctggccgccg
     2161 acccccatgc cgacccggaa gaagtaaagg ccgaatatgg catcgagatc acccgcctat
     2221 cgccggagac gccggtggat gcgcttgtgg tggcggtcgg ccataatgaa taccgggaga
     2281 tgacatcggc ccagcttcgc gcccttacgc gcggcgacaa gccggtgctg tccgacgtca
     2341 agagccttta cgacaggcat gctgcggcaa aggaagggtt ccaggtgttt cgactgtgac
     2401 cacgaataat tcaaacagca aggtgcgttt tgccctggtg ggagccgggc ggattgcggc
     2461 caaccatctc aaggcgatgg aatcgcatgc agaccggtgc gaactggttt ccatctgcga
     2521 caccgacgag caggttcttg ccaagatcgc cgcgcagacc ggcgcaaagg gatttagccg
     2581 gctggaccag atgctggcga atacgccggc cgactgcgtg gtcttgacca cgccgagcgg
     2641 cctgcatgcc tctcaggcaa tcgccgttgc ccaatctggc cgccacgtca tcaccgaaaa
     2701 gccgatggcg acccgttgga aagacggcct ggcgatggtc gaggcctgcg acaaggcagg
     2761 cgtgaagctg ttcgtcgtca agcagaaccg gcgaaacgcc acgctccagc tgctgcggga
     2821 agccgtgcgg cagggacgtt tcggccgcat ctattcggtt tccgtcaacg tcttctggac
     2881 gcggccccag gaatattacg acgccgccaa gtggcgcggc acctgggagt tcgacggcgg
     2941 cgccttcatg aaccaggcca gccactatat cgacctgctg caatggatcg tcggaccgat
     3001 cgaaagcgtg atggcctata ccggcacgct ggcgcgcgac attgaagcgg aagataccgg
     3061 cgttgcggcc ctgcgctggc gcagcggcgc gatgggcacg ctgaacgtga ccatgctgac
     3121 ttatccaaag aacctcgaag gttcgatcac catcctgggc gagaccggaa ccgcgcgcat
     3181 cggcggcgtc gcagtcaacg agatccagga atggtcgttt gccgacaagc gtcccgaaga
     3241 cgacgaaatc aagaacgcga gttatcaaac cacaagcgtc tacggttttg gacatccgct
     3301 ttactacgac aacatcatca agacgctgcg cggcgaggaa gccgccgaaa ccgatggccg
     3361 gcaggggttg aaaagcctgg aactgctggt cgccatgtat atgtcggcgc gcgacggcag
     3421 gcgggtcgcc ctgccgctgg agcactgaca tgaattacaa ggcccatcct tcggccatcg
     3481 tcgacgatgg cgcgcagatc ggcgacgaca cccgcatctg gcattgggtg catgtcagcg
     3541 ccggcgcgcg catagggcgc ggctgctcgc tcgggcaaaa cgtcttcatc gccaacaggg
     3601 tgacgatcgg cgacaacgtc aaaatccaga ataatgtctc ggtctacgac aatgtcacgc
     3661 tggaagacga tgtcttctgc gggccgagca tggtcttcac caatgtctac aacccgcgtt
     3721 ccgccgtttc acgcaaggaa gaataccgcg acacgaccgt gcggcagggt gcgacgctgg
     3781 gcgccaactg cacgatcgtc tgcggcgtga ccatcggcga atatgctttc gtcggcgcag
     3841 gcgcggtggt gagccgcgac gttgccgcct atgcgctgat ggtcggcgta ccggcgcggc
     3901 gcatcggctg gatgagccgg catggcgagc ggctggacct gcccgccagc ggcagcggcg
     3961 aagccgcctg ccccgtcatg ggcgacctct acaggcttca ggacgaccgg gtcgagagaa
     4021 ttgaggcggc accacgatga atacgcaaac cgatgcgcag gcgcccatcc agttcatcga
     4081 cctgaaggcg caatatgcgg acctgcgcgg accggtcaac gcccgcatcc aggcggttct
     4141 cgatcacggc caatacatta tgggaccgga ggtagccgag cttgaagcgg cactttcgac
     4201 ctataccggc gcgaagcact gcatcacggt ttccagcggc accgaagccc tgctcatcgc
     4261 catgatgggg ctgggaatag gccccggcga tgaagtcatc accacgccct tcacctttgc
     4321 ggcgaccgcc gagatgatcg tgctgctcgg tgcgagaccg gttttcgtcg atgtcgaaac
     4381 ggcgacgtgc aacatcgatg ccgggctgat cgatgcgaaa attaccgctc gtaccaaggc
     4441 catcatgccg gtcagccttt atggccaggc cgccgacatg gacgagatca acgaagtggc
     4501 ggcccgccac ggcgtggcgg tgatcgagga tgccgcccaa agcttcggcg ccgattatcg
     4561 cggcaagaag agctgcaacc tttcaaccgt gggctgcacc agcttcttcc ccagcaagcc
     4621 gctgggctgc tatggcgatg gcggcgccat gttcaccaat gacgacgccc tggccaaggc
     4681 tttccgcgaa atacgggtac acggccagag caagcgctat gtgcacacgc gcgtcggcgt
     4741 tggcgggcgc atggacacgc tgcaatgcgc catcgtgctt gccaagctgg aacgcttcga
     4801 ctgggaagtc gggcgccggc tggaacttgg ggcgcgctac aatcggctgc tcgacgaagc
     4861 cggcgtcaag cgggtccatc agaggcagga ccgcaacagc gttttcgcgc aatataccgt
     4921 gctggtcgag aaccggacgg aaatacagga aagcctccag gcaagcggcg ttccgaccgc
     4981 catccactat cccattccgc tcaacctaca gccagcctac gcgcatctga acgtagacgg
     5041 gcagacggtg aattccgaat gggtggccgg ccgcgtgatg agcctgccga tgagcgccta
     5101 tctgaccgag gatcagcaag accggatcgt cgagacgctg aaggcggcgg tatggcggcc
     5161 ctgatcgcca ggctcaaggg tcttgcttca aacgatttca tccgctccgt cggtgttctt
     5221 gtcggtggaa cggcagccgc ccaggtcctg atggcggtga gcctgccgat tgcgacgcgg
     5281 ctctattcac cgaccgattt ctccctgctg gcgctgttta cggcaatcgt ctcgatcatt
     5341 tccgtttccg cctgcctgcg gttcgacatt gcggttccga tcccggaaag cgacgaggag
     5401 gcggccaacg tgctggcgct cgccgtgggg tttgccgtgc tcgtctcggc ggtgctggcg
     5461 cttctggcca ttttcgcgcc gggtccgatc gtgcgcctgt tgaacaggcc ggatttccag
     5521 ccctatctct ggcttgtgcc gctgaccgtc ctgttgtccg gtcttttcag cacgttgcaa
     5581 tactggttcg tgaggcgcaa atcgttcagg gccatcgcca cgaccaagat ggggcaagcg
     5641 acgggcgcct cggcaaccct ggtcggcctc ggtttcgccc acccgacgcc ccttgggctg
     5701 ctgctggcct atgccatcaa ttcaggcgcc ggcttcatcg ggctcagcta tcgcttcttg
     5761 cgcacagacg tcgcgcttct gcgccaggtg agctggcagc ggatgaaaca cgccttcgcc
     5821 gcgaacagcc gctttccgaa atactcgaca ctcgaggcag cctgcaacag cgcctcgatc
     5881 tacctgccga tcatcctgat ttcatcgctc gccgccgggc cggaggccgg ctacgtcatc
     5941 gtcgcaatcc aggtgatgca ggcgccgatg agcctgatcg gctcggcgat cgcgcaggtc
     6001 tatctgtcgc gcgcgccgga agaatttcgt gccgacaacc tcggcgcctt caccgcctcc
     6061 atcttctccg gcctgctcaa aaccggagta ggtccgctga ttttcggcgg catcgtcgca
     6121 cccgaagtct tttcgctggt cttcgggcaa ggatggcggc cggcgggaga actcgtgctg
     6181 tggatgacgc catggttcgt cgcccaattc ctgtccagcc ccctttccat ggcgcttcac
     6241 gtcaccggaa accagcggct ggcgctttgc ctacagatat tcgggctgat cgtcagggcc
     6301 ggctcgatat atgctgccta cctggtcttt ccgaaatacc tttcggagac ctacgcaata
     6361 tccggcttta tattttacag cgtctataat tatcagatct ttagaataac aggaaccggt
     6421 ctttattcct tattcgggtc cctcattaag aactccattt ttctgctgtt ctggatttct
     6481 atggcgataa ttttgaaggt tattttctat atgtattggt aagttctttc aattgatttc
     6541 agccgaacta cgcctaacct agccataatt gtccttgtga ctgcgctgct ctcgatcctt
     6601 ggacagttga gattgcaaac cccacgcaag cgcaggcaaa ggccagaaaa ccgatttcgc
     6661 cacgactatc aaaagacagc aaaaggcact gaccaacaac agcgaccacc atcgcgcatc
     6721 gaagtcccgt ccggcgatgc gccggcgcac cgccgcgata agacaaccgg ttgtcatcaa
     6781 gatgtagagt ataaatggca cgattccaaa ctgatgccac atggatagcg cattgtgtat
     6841 atacgatcca aagtcctttt gacttctgat atagcctcca tagtcgccga atatgggatt
     6901 ggccattata tccttgattc cgccaatcat gatttgccag cgctcgttaa gcgaagctga
     6961 agagccaatg ttcgtgtatc gtgtcagcag ctgcggatga aaatacccaa ctacaaacaa
     7021 cgcgacgata aaaaacatca gaagcgcggc ggcggcagga agtatatatc gccgctgact
     7081 gctcgcggcc aaccatacca tgacgacaaa catataaccg aatagctcgg cccgagcgcc
     7141 agtatatgcc aaggccaacg ctcccagcac caccacgacc actcgaagga cgaccgttgt
     7201 ttccaatgcc aatgcgaata tggctgtgaa cgcgatggcg ttcgcaaacc atgcgtagct
     7261 cgcaacctgc ccttgaaacc tccaacgcaa cgcttggtca ggcgacagag gatccgttgc
     7321 cataacgaat ggaattagca cagccatgac aacgagcgat gcagtcaaca aatgagcaag
     7381 gcgctcactg ggtcgtagaa aatacccagc accgtaaagc gaaatccagc agataagcag
     7441 ctttgtcgac tcaaaggcag aaatattccg atcatacggc
//
LOCUS       DASYCS010000242         9753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4263732,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000242 DASYCS010000000
VERSION     DASYCS010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 9753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9753
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4263732"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1031
                     /gene="cysC"
                     /locus_tag="VGM46_07760"
     CDS             <1..1031
                     /gene="cysC"
                     /locus_tag="VGM46_07760"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019857048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEY4192528.1"
                     /translation="SGQAVTLVLDDELEVSRGNMLVSPTARPQVADQFAANIVWFDEN
                     ALLPGRSYILRTETDQASATVTDLKYRVNINDFAHEAAKSLGLNEVGTCNISTRTPIV
                     FDAFSENRATGAFILIDRITNATVGAGMILHALRRAENIHWQSLDVGKRERSDIKNQR
                     PAVFWFTGLSGSGKSTIANLLEKKLHAAGRHTYILDGDNVRHGLNRDLGFTDADRVEN
                     IRRVAEVARLMSDAGLIVLVSFISPFRAERRMARELMAQGEFVEVFVDTPFEECARRD
                     TKGLYARALNGEIKNFTGVDSPYESPENPEIHLKTLGRSPEEMVEALEQWLIERDSAE
                     DQYDGGGI"
     gene            1042..1827
                     /gene="cysQ"
                     /locus_tag="VGM46_07765"
     CDS             1042..1827
                     /gene="cysQ"
                     /locus_tag="VGM46_07765"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018426910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HEY4192529.1"
                     /translation="MLDVFERLALAAGREVMRIFHAGCAVDHKADSSPVTEADKASEA
                     IILAGLRAAFPDIPCVAEEEACAGIVPPDLDGMFFLVDPLDGTKEFINRRTDFTVNIA
                     LIRRGAPEIGVVFAPCSGRFFSGLPGRAEALDLGEDNEIRARHPISARVGAQPLAIVA
                     SRSHNTPETEAYIRSLGAAEIVSVGSSLKFCLLASAEADVYPRFGRTMEWDTAAGDAV
                     LRAAGGMTRTLDGNPLVYGKRNQPHDEDFANPHFIASGRVQQP"
     gene            complement(1834..2973)
                     /locus_tag="VGM46_07770"
     CDS             complement(1834..2973)
                     /locus_tag="VGM46_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018426911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEY4192530.1"
                     /translation="MQFIDLGAQRERIRDRLKAAIDRVVDEGRYILGPQVAEFEAKMA
                     AYIGTKHVIACANGTDALLLPLMASGIGPGDAVFVPSFTFAATAEVVALTKAEPVFVD
                     VDPKTYNIDIASLEAAISMIKSEGRLKLKAIIPVDLFGLCADYDALMAIATRENLLVI
                     EDAAQSIGGSVDGKMCGAFGHVAGTSFYPAKPLGCYGDGGAMFTNDDALADRLRSFAF
                     HGKGETQYDNIHVGINSRLDTMQAAILIEKLAILEDEMEARRNVAQRYADGLGDIVTA
                     ANNPAGGRSAWAQYAIETPKRDGLRAHLTEKGIPSVIYYVKPLHSQVAYNSYPKTPTG
                     LAVSEALPNRILCLPMHPYLSEADQDQIIETIRNYIGSNSAQAQE"
     gene            complement(3202..4125)
                     /locus_tag="VGM46_07775"
     CDS             complement(3202..4125)
                     /locus_tag="VGM46_07775"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010914959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HEY4192531.1"
                     /translation="MILCCGEALIDMLPRQTPAGEPAFAPYAGGALFNTAIALGRLGA
                     PVEFFSGLSTDLFGRQLTRVLAESKAGARYAHFSPRPTTMAFVQLNDGQASYVFYDEG
                     TAGRMLSQSDLPDLGADVDALLFGAISLIPEPCGSTYEALMAREHAHRVIMLDPNIRP
                     GFIPDRAGHAGRIRRMVGMADIVKLSDEDLDWFGETGSREEIVARWLERGPKLILITC
                     GGDGAIGYTKNHRVAIRPDKVEVVDTVGAGDTFNAGILASLHEQGVLSKPALADLSEE
                     AIAKALSLGAKAAAVTVSRAGANPPWRSEIS"
     gene            complement(4128..4568)
                     /locus_tag="VGM46_07780"
     CDS             complement(4128..4568)
                     /locus_tag="VGM46_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006330863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0048029 - monosaccharide binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU domain-containing protein"
                     /protein_id="HEY4192532.1"
                     /translation="MLIGIPALLGPDLLAALRAMGHGDEIAIVDGNYPALTDARRLLR
                     ADGHGVIQVLDAILTVLPVDDMVPAAIFRASVKGDPALADPVHREMEAICATHAPGRP
                     VTALSGPDFYARVKAAHAVVATSEARLYANIIVRKGVIYPAGDR"
     gene            complement(4571..5809)
                     /locus_tag="VGM46_07785"
     CDS             complement(4571..5809)
                     /locus_tag="VGM46_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEY4192533.1"
                     /translation="MQTVADRNGSPEFGNSRFHRGTNQSGMRDHNERLVLSLVRQSGS
                     LAKSDIARMTGLSAQTVSVIMRQLEDDGLLVRQPPLRGKIGQPSIPMALNPQGAYFFG
                     LKIGRRSAEMVLIDFLGNVLARTQRAYRYPTTGEALAFAADSLKEMRDGLDPAQDQRI
                     AGLGIAMPYELWNWADAIGAPEAVREEWRQADIRSEIQSLCPFPVFLQNDATAACGAE
                     LVFGQAGGLRNFVYFYIGAFAGGGIVLDGRLYGGPTGNAGALGSMPVPRPASGPDDRP
                     AQLIDIASIAMLEKALVAQGRDAGRLWESPEDWGDIGPALPAWIASAAEALAYAIAAA
                     ASVIDFEAAVIDGWMPLSTRRQLVAAVEAAIGRIDGEGLNFPAIREGTIGIHARAMGG
                     ASLPLSERFLVGPTTPSRSL"
     gene            6012..7043
                     /locus_tag="VGM46_07790"
     CDS             6012..7043
                     /locus_tag="VGM46_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018094081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEY4192534.1"
                     /translation="MKKSVLRAALLGASALGLAAMAAPASAADASACLITKTDTNPFF
                     VKMKEGATAKAKEIGVNLKTYAGKIDGDSESQVAAIESCIADGAKGILITASDTAGIV
                     PAVKKARDAGILVIALDTPLDPADAADATFATDNLLAGELIGKWAAATLGDKAKDAKI
                     AFLDLTPSQPTVDVLRDQGFMKGFGIDTKDINKIGDEDDARIVGHDITNGNEEGGRKA
                     MENLLQKDPSINVVHTINEPAAAGAYQALKAVGVEKNVLIVSIDGGCPGVKNVEAGVI
                     GATSQQYPLMMAALGIEAIKKFADSGEKPKPTDGKSFFDTGVALVTAKPAAGIDSIDV
                     KTGLEKCWG"
     gene            7154..8233
                     /locus_tag="VGM46_07795"
     CDS             7154..8233
                     /locus_tag="VGM46_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013635210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4192535.1"
                     /translation="MSEASAEARQQDFEKALSGSEGAVANFDAGRRPALEKMQHFLHA
                     SPAAVPLIVLVFSLVAFAAIIGGKFFSAFSLTLILQQVAVVGIIGAAQTLVILTAGID
                     LSVGAIMVLSSVVMGQFTFRYGLPVPVAVLCGFGVGALCGWLNGFLVARMKLPPFIVT
                     LGTWQIVLATNFLYSANETIRAQDIETNAPLLQFFGQNIRLGNAVFTYGVIIMVLLVM
                     LLWYVLNHTAWGRHVYAVGDDPVAAELSGVNVTRVLISVYVIAGLICALAGWALIGRI
                     GSVSPTSGQFSNIESITAVVIGGVSLFGGRGSILGMLFGALIVGVFSLALRLIGTDPQ
                     WTYLLIGTLIIVAVAVDQWIRKVAG"
     gene            8209..9009
                     /locus_tag="VGM46_07800"
     CDS             8209..9009
                     /locus_tag="VGM46_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY4192536.1"
                     /translation="MDQKGSRVMEPLLQARGLVKRYGRVTALDHADFDLYPGEILAVI
                     GDNGAGKSSLIKAISGAVIPDEGEIRLDGKVISFKSPMEARKAGIETVYQNLALSPAL
                     SIADNMFLGREIRKPGFMGQWLRMLDRPAMEKQARDKLSELGLMTIQNISQTVETLSG
                     GQRQGVAVARAAAFGSKVIILDEPTAALGVKESRRVLELILDVKKRGLPIVLISHNMP
                     HVFEVADRIHIHRLGRRLTVIDPKAYTMSDAVAFMTGAKASPDALVAA"
     gene            9220..>9753
                     /locus_tag="VGM46_07805"
     CDS             9220..>9753
                     /locus_tag="VGM46_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019857058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HEY4192537.1"
                     /translation="MLKPGLTNSDPKTLAGEVLKALKYRVGKDTTVATPHDWLTASIV
                     VVRDRIVDHWIEATKEAYDHHKKRVYYLSLEFLIGRLMRDAFSNLGLMEDMRAALSSL
                     NVDLDVIAALEPDAALGNGGLGRLAACFMESMASVDIPAHGYGIRYANGMFRQEIQDG
                     WQVELPETWLDHGDPWEF"
BASE COUNT         1764 a         3063 c         3110 g         1816 t
ORIGIN      
        1 tatccggcca ggcggtgacg ctggttctgg acgatgagct ggaggtctcg cgcggcaata
       61 tgctggtgtc gccgacggca cgcccccagg tcgccgacca gttcgccgcc aacatcgtct
      121 ggttcgacga gaatgcgctg ctgccggggc gctcctatat attgcgcacc gaaaccgacc
      181 aggcgagcgc taccgtcacc gacctgaaat accgcgtcaa catcaatgat ttcgcgcatg
      241 aggcggcgaa gtcgcttggc ctgaacgagg ttggcacctg caacatctcg acgcgcacgc
      301 cgatcgtctt cgacgccttc tcggaaaacc gcgcgaccgg cgcgttcatc ctgatcgacc
      361 gtatcaccaa cgcgacggtc ggcgccggca tgatcctgca cgcgctgcgc cgggccgaaa
      421 acatccattg gcaatcgctc gacgtgggca agcgcgagcg ttccgatatc aagaaccagc
      481 ggccggcggt gttctggttc accggcctgt cgggttcggg caaatccacc atcgccaatc
      541 ttttggaaaa gaagctgcat gcggcgggcc gccataccta tattctggat ggcgacaatg
      601 tccggcacgg attgaaccgc gatcttggct tcaccgatgc cgaccgtgtc gagaacatcc
      661 ggcgggtggc cgaagtggcg aggctgatga gcgatgccgg gctgatcgtg ctggtgtcgt
      721 tcatttcgcc gttcagggcc gagcggcgca tggcgcgcga gttgatggcg caaggcgagt
      781 tcgtggaagt gtttgtcgac accccgttcg aggaatgcgc caggcgcgac accaagggcc
      841 tgtatgcgcg cgccttgaac ggcgagatca agaatttcac cggcgtggat tcaccctatg
      901 aatcaccgga aaatcctgaa atccatctca agacacttgg gcgttcgccc gaagagatgg
      961 tagaagcgtt ggaacaatgg ctgatcgagc gcgacagtgc cgaggatcaa tatgacggcg
     1021 gcggcatctg aagacacccg gatgctggat gtgttcgagc gccttgcctt ggcggcaggg
     1081 cgcgaggtga tgcgtatttt ccatgccggt tgcgcggtcg accataaggc cgacagttcg
     1141 cctgttaccg aggccgacaa ggccagcgag gcgatcattc tggctggcct gcgcgccgcg
     1201 ttccccgaca ttccctgcgt tgccgaggaa gaagcctgcg ccggcatcgt gccgcccgat
     1261 ctggacggta tgttctttct ggtcgatccg ctcgacggca cgaaggaatt cataaaccgc
     1321 cgtaccgatt tcaccgtcaa catcgcgctt atccggcgcg gcgcgcctga aatcggcgtg
     1381 gttttcgcgc catgcagcgg ccgcttcttt tccggcctgc cgggcagggc cgaggccctc
     1441 gaccttggcg aggacaatga aatccgcgcc cgccatccga taagcgccag ggtcggcgcg
     1501 caaccgctgg ccatcgtcgc cagccgttcg cacaacacgc cggaaacgga agcctatatc
     1561 cgctcgctgg gcgctgccga gattgtctcg gtcggctcgt cgttgaagtt ctgcctgctg
     1621 gccagcgccg aggcggacgt ctatccgcgc ttcggccgca ccatggagtg ggatacggcg
     1681 gcgggcgatg cggtgctgcg cgccgccggc ggcatgacgc ggacgctgga cggaaacccg
     1741 ctcgtctacg gcaagcgcaa ccagccgcat gacgaggatt tcgccaatcc ccacttcatc
     1801 gccagcggca gggtgcagca gccctgaagc tggttactct tgcgcctgcg ccgagttcga
     1861 gccgatataa ttgcggatcg tctcgatgat ctggtcctgg tcggcttcgc tgagataggg
     1921 atgcatcggc aggcacagta tgcggttggg cagcgcttcc gaaacggcaa ggccggtcgg
     1981 tgttttcggg tagctgttgt aggccacctg cgaatgcagc ggcttcacat aatagatgac
     2041 cgaggggata cccttttcgg tcagatgcgc ccgcaagccg tcgcgcttcg gcgtctcgat
     2101 ggcgtattgc gcccaggccg agcgcccgcc ggctggattg ttcgccgccg tgacgatgtc
     2161 gccgaggccg tccgcatagc gctgcgccac attgcggcgg gcttccattt cgtcttcgag
     2221 aatggccagc ttttcgatca ggatcgccgc ctgcatcgtg tcgaggcgcg aattgatgcc
     2281 gacatggatg ttgtcatact gcgtctcgcc cttgccgtgg aaggcgaagg agcgcagcct
     2341 gtcggccagc gcgtcgtcat tggtgaacat cgcgccgcca tcgccgtaac agccaagcgg
     2401 ctttgccggg tagaagctgg tgcctgcgac gtgcccgaaa gccccgcaca tcttgccgtc
     2461 gacggagccg ccgatcgatt gcgccgcatc ctcgatgacc agcaggttct cgcgcgtggc
     2521 gatggccatg agggcgtcgt agtcggcgca aagcccgaac agatcgaccg gaataatcgc
     2581 cttcagcttc agccggcctt cgctcttgat catcgaaatc gccgcttcga ggctggcgat
     2641 gtcgatattg taggtctttg gatcgacatc gacgaaaacc ggctccgcct tggtcagcgc
     2701 caccacttcc gccgtggcgg caaaggtgaa gctcggcacg aacaccgcgt cgccggggcc
     2761 gatgcccgac gccatcagcg gcagcaaaag cgcgtcggtg ccgttggcgc aggcgatcac
     2821 atgcttggtg ccgatatagg cggccatctt ggcctcgaac tcggccacct gcggtcccag
     2881 aatatagcgc ccttcatcga cgacgcggtc gatggccgcc ttcaggcgat cacggatacg
     2941 ttcgcgctgt gcgccaaggt cgatgaattg catgtctgtt ctctaagaat gtttgcccgt
     3001 taacgggcgc tggtatcaga attgcgcgga tcgagaaagc attcggcggt gtggcgtggt
     3061 tgcgcagcca ccccgttgcg ttacttggtc cagccgggcc acgcacctca atagggtgaa
     3121 aatccggggt ttgccaaggc aggcttatcg ccgcgatcta tcgagggcgt ttgcgcgcaa
     3181 cacaaagtga ttgcggcggc gtcaggaaat ctcgctgcgc catggcggat tggcgcccgc
     3241 gcgcgacacg gttaccgcag ccgccttcgc gccgagcgac agcgccttgg cgatggcttc
     3301 ttcggacagg tcggcaaggg ccggtttcga cagcacgccc tgctcgtgca gcgaggcgag
     3361 gataccggca ttgaacgtat cgccggcgcc caccgtatcg accacctcga ccttgtcggg
     3421 gcggatggcg acgcggtggt ttttcgtgta gccgatggcg ccgtcgccgc cgcaggtaat
     3481 caggatcagc ttcggtccgc gttcaagcca gcgcgctacg atttcctcac gtgatcccgt
     3541 ttcgccgaac cagtcgagat cctcgtcgga cagcttgacg atatcggcca ttccaaccat
     3601 gcgacggatg cgcccggcat ggccggcccg gtcgggaatg aagcccggcc ggatgttggg
     3661 gtcgagcatg atgacgcgat gcgcgtgctc ccgcgccatg agcgcctcat aggtggagcc
     3721 acacggttca gggatcaggc tgatggcgcc gaacaaaagc gcgtcgacgt ccgcgccaag
     3781 gtcgggcagg tcggactgcg acagcatccg cccggcggtg ccttcgtcgt agaagacata
     3841 gctcgcctgg ccgtcgttga gctgcacgaa agccatggtg gttggccttg gcgagaaatg
     3901 ggcatagcgc gcgccggcct tgctttcggc cagcacccgc gtcagctgcc gtccaaacag
     3961 gtcggtggat agcccggaga agaactccac cggcgcgccg agccgcccga gcgcgattgc
     4021 ggtgttgaaa agcgcgccgc ctgcataggg cgcgaatgcc ggctccccgg cgggcgtctg
     4081 gcgcggcagc atgtcgatga gcgcctcgcc gcagcaaagg atcatgatta tcggtctccg
     4141 gcaggataaa tgacgccctt gcggacgatg atattggcgt agaggcgcgc ttcgctggtg
     4201 gcgaccacgg catgcgcggc cttcaccctg gcgtagaaat cagggccgga aagagccgtc
     4261 accgggcggc ccggcgcatg tgtggcgcat atcgcctcca tttcccggtg cacgggatcg
     4321 gccagcgcgg ggtcgccctt gaccgaggcg cggaagatgg cggccggcac catgtcgtcc
     4381 accggcagca cggtcaaaat ggcgtccaga acctgaatta caccatggcc gtctgcacgc
     4441 agcaggcggc gcgcgtcggt aagcgccggg taatttccat cgacgatggc gatctcgtcg
     4501 ccatggccca tggcgcgaag cgcggccagc aggtcggggc cgagcagggc cgggatgccg
     4561 atcagcatgg ctacaagctc cgcgaagggg tggtcgggcc gacgaggaag cgttcggaaa
     4621 gcggcaggct cgcaccgccc atggcgcggg catggatgcc gatggtgcct tcgcggattg
     4681 ccggaaagtt caggccctcg ccgtcgatcc tgccgattgc ggcctcgacg gccgccacca
     4741 actgacgccg tgtcgacagg ggcatccagc catcgattac ggctgcctcg aaatcgatga
     4801 ccgaagcggc ggcggcgatc gcgtaagcca gcgcctcggc cgcgctggcg atccaggctg
     4861 gcagggccgg cccgatgtcg ccccagtctt ctggcgattc ccacaagcgc cccgcatcgc
     4921 gcccttgcgc gacgagcgct ttttccagca tcgcgatgga ggcgatgtcg atcagctggg
     4981 ccggcctgtc gtcggggccg gatgcgggcc ggggcaccgg catcgaaccc aatgcgccgg
     5041 cattgcccgt tggcccgcca taaagacgcc catccaggac gatgccgccg ccggcaaacg
     5101 cgccgatgta gaaatagacg aagttgcgca gcccgcccgc ttgcccgaac accagttcgg
     5161 ccccgcaagc ggcggtggcg tcgttctgga ggaaaaccgg gaaggggcac agcgactgga
     5221 tttcgctgcg aatgtcggct tgccgccatt cttcccgcac cgcttcgggc gcgccgatgg
     5281 catcggccca gttccacaat tcgtagggca tggcgatgcc gagaccggca atgcgctggt
     5341 cctgcgcggg gtcaagcccg tcgcgcattt ccttcagcga gtccgccgcg aaggccaggg
     5401 cctcgccggt cgtcggatag cgataggcgc gctgcgtgcg ggccagcaca ttgcccagga
     5461 aatcgatcag caccatttcg gcgctgcggc ggccgatctt caggccgaag aaatacgcgc
     5521 cctgcgggtt gagcgccatc ggtatggatg gttggccgat cttgccgcgc aacggcggtt
     5581 gccgcacaag cagcccgtca tcctccagct ggcgcatgat gaccgacacc gtctgcgccg
     5641 acaggccggt catgcgggcg atgtcggact tcgccaggct tccgctctgg cgcaccagcg
     5701 aaagcaccag ccgctcattg tggtcgcgca tgccgctctg gtttgttccc cggtggaagc
     5761 ggctgttgcc gaactcgggc gaaccgttcc tgtcggctac ggtttgcacc ccgttcctcc
     5821 tgaatatgct cttgacgcaa tcgattcaga atgggtcggc ggcacgaatg tgtcaatgat
     5881 aaataagagt gatttaatta ttgactcgca caagtcgcgg gtgtcttgtg acgaagcggg
     5941 cgggcaagtt tgagcaccgc acgatggccc ggtatggtcc gggatggttt gctttctggg
     6001 aggatttacc gatgaagaaa tctgttctgc gcgcggcctt gctgggcgcg tcggcgctgg
     6061 gtcttgcggc gatggccgct ccggcttccg ccgccgatgc ctctgcctgc ctgattacca
     6121 agaccgacac caacccgttt ttcgtcaaga tgaaggaagg tgcgacggcc aaggccaagg
     6181 aaatcggcgt caatctgaaa acctatgccg gcaagatcga cggcgacagc gaaagccagg
     6241 tggcagccat tgaaagctgc atcgccgatg gcgccaaggg catcctgatc accgcgtccg
     6301 acacggccgg catcgttccc gccgtcaaga aggcgcgcga cgccggtatc ctcgtcatcg
     6361 cgctcgacac gccgctcgat cccgccgacg ccgccgacgc caccttcgcc accgacaatc
     6421 tgctcgccgg cgaactgatc ggcaaatggg ccgccgcaac ccttggcgac aaggccaagg
     6481 acgccaagat cgccttcctc gacctgacgc cttcccagcc caccgtcgac gttctgcgcg
     6541 accagggctt catgaagggc ttcggcatcg acaccaagga catcaacaag atcggtgacg
     6601 aggacgatgc ccgcatcgtc ggccacgata tcaccaacgg caacgaggaa ggcggccgca
     6661 aggccatgga aaacctcctc cagaaagacc cgtccatcaa cgtcgtccac accatcaacg
     6721 agccggccgc ggcaggcgcc tatcaggcgc tcaaggctgt cggtgtggag aagaacgtcc
     6781 tgatcgtatc gatcgacggc ggttgcccgg gcgttaagaa cgtcgaggcc ggggtgatcg
     6841 gcgccacctc gcagcagtac ccgctgatga tggcagccct cggcatcgag gcgatcaaga
     6901 agttcgccga cagcggcgaa aagccgaagc cgaccgacgg caagagcttc ttcgacacgg
     6961 gcgttgcgct ggtcacggcc aagccggccg ccggcatcga ctccatcgac gtcaagaccg
     7021 gcctggagaa atgctggggt tgatataggc gtccatcggt cggactatcg tcatgacggc
     7081 gaagggtggc atcgaccgcc cttcgctcga ccgggcgggc agtcctgacg ggcagcccta
     7141 aaaggaggat tccatgagcg aagcttcggc ggaagcccgg cagcaggatt ttgagaaagc
     7201 gctttccggc agcgagggcg cggtggcgaa tttcgacgcc ggcaggcgtc ccgccctgga
     7261 gaagatgcag catttcctgc atgcaagtcc ggctgccgtt ccccttatcg tccttgtctt
     7321 ttcgctcgtg gctttcgccg caatcatcgg cggcaagttc ttctcggctt tttcgctgac
     7381 gcttatcctg caacaggtgg cggttgtcgg catcatcggc gccgcccaga ccctcgtcat
     7441 cctgactgcc ggcatcgacc tgtcggtcgg cgccatcatg gtgctgtcct cggtggtgat
     7501 ggggcagttc accttccgct acggtcttcc cgtgccggtc gccgtgctgt gcggcttcgg
     7561 cgtcggcgcg ctgtgcggat ggctgaacgg ctttctcgtc gcgcgcatga agctgccgcc
     7621 attcatcgtc acgctcggca cctggcagat cgttttggcg accaactttc tctattcggc
     7681 aaacgagacg atccgggcgc aggatatcga gaccaacgcg ccgcttctcc agtttttcgg
     7741 ccagaacatc cggctgggca acgcggtttt cacctatggc gtcatcatca tggtgctgtt
     7801 ggtcatgttg ctctggtatg tgctcaatca caccgcctgg ggccggcatg tctatgccgt
     7861 gggcgacgac ccggtcgccg ccgaactttc gggcgtcaac gtcacccgcg tcctcatttc
     7921 cgtctatgtc atcgccgggt tgatctgcgc gctggccggc tgggcgttga ttggccgcat
     7981 cggctcggtg tcgcccacgt ccggtcagtt ctccaacatc gaatcgatca ccgccgtggt
     8041 gatcggcggc gtctcgctgt ttggcgggcg cggctcgatc ctcggcatgc tgttcggcgc
     8101 gctgatcgtc ggcgtctttt cactggcgct gcgcttgatc ggcaccgatc cgcaatggac
     8161 ttacctgttg atcggaacgc tcatcatcgt cgcggtcgcc gtcgaccaat ggatcagaaa
     8221 ggtagccggg tgatggagcc tcttcttcaa gcgcgcggtc tggtgaagcg ctatggccgc
     8281 gtcacggcgc tcgaccacgc cgattttgac ctctatccgg gcgaaatcct tgccgtcatc
     8341 ggtgacaatg gcgccggcaa atcatcgctg atcaaggcga tttccggcgc ggtcattccc
     8401 gacgagggcg aaatccgcct cgacgggaag gtgatttcct tcaagtctcc gatggaagcg
     8461 cgcaaggccg gcatcgaaac ggtctaccag aacctggctt tgtcgccggc actgtcgatt
     8521 gccgacaaca tgttcctggg cagggaaatc cgcaagccgg gcttcatggg ccaatggctg
     8581 cggatgctgg accgcccggc catggaaaaa caggcgcgcg acaagctttc cgagcttgga
     8641 ttgatgacga tccagaacat cagccagacg gtggaaacgc tgtccggcgg ccagcggcag
     8701 ggcgttgccg tggcccgcgc cgcagcattc ggctccaagg tcatcatcct ggacgaaccg
     8761 acggcggcgc ttggcgtcaa ggaaagccgc cgcgttctgg aactgatcct cgacgtcaag
     8821 aaacgcggcc tgccgatcgt gctgatctcg cacaacatgc cgcatgtttt cgaggtggcc
     8881 gaccgcatcc atatccaccg gctcgggcgt cgtctgaccg tcatcgatcc caaggcatat
     8941 accatgtcgg acgcggtggc tttcatgacc ggcgcaaagg cgtctcccga cgcgctggtc
     9001 gctgcctgat ccgccgcgat ccatatgccg gccatggtta tgtgccggca tatgtcggct
     9061 tgtttcggga aacgtttctt tgctgatcaa tttaaatcga ttgaagaaat ggccgggcca
     9121 gctagcctga ggccgggatg acggatagat caaaggttcg ccgccgctcg ggcgacgaac
     9181 ctttaaaaaa agcggaacga acgcgtggaa agctccgaaa tgctcaaacc cggcctcacc
     9241 aattccgacc cgaagacgct ggcgggcgaa gtgctcaagg cgctcaaata tcgggtcggc
     9301 aaggacacca ccgtcgccac cccgcatgac tggctgaccg catccatcgt ggttgtgcgc
     9361 gaccgcatcg tcgatcactg gatcgaggcg accaaggaag cttacgatca tcacaagaag
     9421 cgggtttatt atctgtcgct cgaattcctc atcgggcggc tgatgcgcga tgccttctcc
     9481 aatctcggcc tgatggagga tatgcgcgcc gcgctgtcgt cgctgaatgt cgatctcgac
     9541 gtgatcgcgg cgctggaacc ggacgccgcc cttggcaatg gcggtctggg ccggctcgcc
     9601 gcctgcttca tggaatccat ggccagtgtc gacattccgg ctcacggcta tggcatccgc
     9661 tatgccaacg gcatgttccg gcaggaaatc caggatggct ggcaggtcga attgccggaa
     9721 acctggctcg accatggcga cccgtgggaa ttc
//
LOCUS       DASYCS010000243         1892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_783015,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000243 DASYCS010000000
VERSION     DASYCS010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783015"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(228..1613)
                     /locus_tag="VGM46_07810"
     CDS             complement(228..1613)
                     /locus_tag="VGM46_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019997674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY4192538.1"
                     /translation="MGAGIAGLTAATELKRRGLPVIVFEAGKGIGGLASSFKDKDGFA
                     YDFGAHFVSNRLASLLGARNICRTVKHYGEAVHVDGRSYGYPFGLMSVPRFAAGALRE
                     RFGERPPMRTAGDWFRRKYGEALAREVAIPLTEAWSGADVDDLALTVGHKFGPRILKA
                     LYLGAAAKITRRAVCNGYSHEMPESSDVYHVYPEGGISKLLEPMAKQAADFIRLESPV
                     EGILTDGDRVEAVRVDGREVPVSAVISTAPVNILPRIVKGTSALDRMAKFRYRPMIFV
                     NLHFEGRGILPDTMLWVPDRSKCFFRMTETPISMPWLAPEGKTLITFDIGCEMSDAHW
                     TMSDEDLAKLCLAGISDIFPKAGSSFIRAGGVVRTPVSYPVYLQEYEDIRRQFAASTG
                     VDGLYSIGRNGEFAHILMEDVYWRTLRRCADVAAYVEGGRQRIDDSVELAPSALDPLP
                     ELAEEQKHAAA"
BASE COUNT          382 a          597 c          569 g          344 t
ORIGIN      
        1 cttggccaca tagggtccga tcttgaagtc ggcttgcccg cgccggaccg gccccgtgcc
       61 gacctcgttt cgggttatta tacaaggtga tatgggggca gcctgcgctg tgcaagcgaa
      121 aagtcggtcg ctccgcgagc acgccggggc cagagaccgg cgcccgcgcc gcgaaccggg
      181 ggcaggaacg cgaaccatcg acggatcgcc gcccgcgtcc gcgccgctca tgcggccgcg
      241 tgtttttgtt cctcggccag ttccggcaag ggatcgaggg ccgaaggcgc cagttcgacg
      301 ctatcgtcga tccgctgcct gcctccctcg acataggcgg cgacatcggc gcagcgccgc
      361 agcgtgcgcc aatagacgtc ttccatcaat atatgcgcga actcgccgtt gcggccgatg
      421 ctgtagaggc cgtccacgcc tgtcgaggcg gcgaactggc ggcgaatatc ttcatattcc
      481 tgaagataga ccgggtagga aaccggcgtt cgcacgacgc cgccagcccg aatgaagctg
      541 cttcccgctt tcgggaaaat atcgcttatc ccggccaggc acagctttgc caaatcctcg
      601 tcgctcatcg tccaatgggc gtcgctcatc tcgcagccaa tgtcgaaagt gatcagcgtt
      661 ttgccttccg gagcaagcca gggcatcgag atcggcgttt cggtcatccg gaagaagcat
      721 ttcgaacggt cgggcaccca aagcatcgta tccggcagga tgccgcgccc ctcgaaatgc
      781 aggttgacga agatcatcgg ccggtaacga aacttggcca tgcggtcgag cgcgctggtg
      841 cctttgacga tcctgggcag gatattgacc ggcgcggtac tgatcacggc ggaaaccggc
      901 acctcccggc catcgacgcg aacggcttcc accctgtcgc catcggtcag gatgccttcc
      961 acgggcgatt ccagccggat gaaatcggcc gcctgtttgg ccatcggctc caaaagcttg
     1021 gaaatgccgc cttcgggata gacatggtag acgtcgctgc tttcaggcat ttcgtgggaa
     1081 tagccgttgc aaaccgccct gcgggtgatc ttagcggcgg cgccgagata gagagccttg
     1141 aggatgcgcg gcccgaactt gtgcccgacg gtaagggcga gatcgtccac atccgccccg
     1201 gaccaggctt cggtcaacgg tatcgccacc tcgcgggcca gcgcctcgcc atatttgcgc
     1261 cggaaccagt cgccggccgt ccgcatcggc ggcctttcgc cgaagcgctc ccgcaaggcg
     1321 ccggcggcga agcgcggcac ggacatcagc ccgaacggat agccgtagga gcggccatcg
     1381 acgtgaacgg cctcgccata atgcttcacc gtgcggcaga tatttcgcgc gcccagcagc
     1441 gaagccagcc ggttgctgac gaaatgagcg ccgaaatcat aggcaaagcc gtccttgtcc
     1501 ttgaacgagg aggcaaggcc gccaattcct tttccggctt cgaagacgat gacgggcagg
     1561 ccgcgccgtt tcaattcagt tgcggcagtc aaaccggcaa tgcccgcacc cacaacagcg
     1621 attggtttca agatagtccc acccgaagaa ttcagggaat gactacagca agggattgaa
     1681 catcatccaa acccggacaa tcgaattgta tctatttcgc cgcaaagccg atccggcacg
     1741 cgccaccggc agctttccgc cgcccaaaac gcgaaaaacc gcggcattcg ggcggctgaa
     1801 gcatggacct cacggtgcat cttgcctatg agttcaagct ggttctcgga aggcagatat
     1861 tttgcaaatt gtcattcttg caggtggctt gg
//
LOCUS       DASYCS010000244        11223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4116966,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000244 DASYCS010000000
VERSION     DASYCS010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 11223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11223
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4116966"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..938)
                     /locus_tag="VGM46_07815"
     CDS             complement(78..938)
                     /locus_tag="VGM46_07815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY4192539.1"
                     /translation="MSRPVVVVTGGASGIGAACCKVIADAGLHVVVADVSEAAGKQVA
                     SDVGGDFVAIDVGDAAGVDAAAQAIEARFGAVAGLVTSAGVIQPPLPPEELDLGLWDR
                     IVHIDQRGTYVTCLAFGRRMAARGTGGAIVNIASVAGSRSMPLHSYAPAKAAVISMTA
                     CLASAWGRDGVRVNSVSPGYTATPGLQTEIDAGRRDVSRLVGNAPLGRLVQPSEIGQA
                     VAFLLSPAASAISGIDLPVDCGWLAGSTWATYGGLPSPQQGVATCRGRPRRMASRAIR
                     GCSTFIAPCC"
     gene            1910..2881
                     /locus_tag="VGM46_07820"
     CDS             1910..2881
                     /locus_tag="VGM46_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4192540.1"
                     /translation="MKGFAFDQQMIVLLLFAVIFAAFSIFLSGFFTVGNLLTLMRTVS
                     VLGILGLGMAVVVIGRGIDLSMIASLAVPSAVVLMLASKDYGIAGSLLCGVALALAIG
                     VLNGFLIAYAEIPSLFTTLAVGIGIAGIGQSGLFEYEIVPWPTQLASISWIGQGSLFG
                     VPYSVLAFVVAAIVVGIFMRKTRLGAFIYASGDNPNTARIAGISVRPILVLQYMIASL
                     IAMFAGLVLAASSAGMDTRIFNLTWIYDVILVVVLGGIGLSGGRGGVLNVVIGTLLIG
                     TIVNGMTIMNVSFEMQNLIKGMVLLAALSIDSIVNPRNEETAQQGDI"
     gene            2932..3936
                     /locus_tag="VGM46_07825"
     CDS             2932..3936
                     /locus_tag="VGM46_07825"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEY4192541.1"
                     /translation="MGLKKIGLAIAMAATVLTGAAQAQNSMNDPARPGYYENLKGRRV
                     VFVPVFMGLDLTEGWSKMMARQAEKLGYKYEVRNSNFNTAAGAQTLTSLITEKPDVIV
                     VQNPDVQSYAKLLAKAEKAGIHVIQLNMKTNVLTTGFVGADVEELGELQAKEVVAHCG
                     TGTSGKVLVLAGPPSSPFSAYMLKGYENILNAAKDVKVVSVQSTGDYESAKAKSITQV
                     VLQQHPDLCGVLGVWDNADVGTAAAIKEAGKSDQVFVSSSGGGGELACKGIQEGMWQH
                     YVSYDVPGQARDLNNLILASLQDKNPVGTTKTILYTPLVIYNKDNLKNQFCWTLDTIR
                     "
     gene            4059..6161
                     /locus_tag="VGM46_07830"
     CDS             4059..6161
                     /locus_tag="VGM46_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY4192542.1"
                     /translation="MSNTLTKLRYRLWPDHVLGELLQKQWMETAVPLLVLLLTIAFFS
                     SRIDNFISVANLSDTLRQASELGFVVLGMSLVLIVGGIDLSVASVFALCNLTALYCIN
                     VLGLPTPAAIVLTLLLGAVLGAVNGVLIGYMRMRAFLTTMVTLIVYKAIHDLLNFKLA
                     VAISSVVPDVPAWDYLGGGDFLSIPMVVWVFAAIAIASHIFLTRLRHGWYVMAIGGNR
                     RSAYNIGLPVNRVVAMSYVASGVFAAISAIFYASRLASPGADTGKGLEITVLTAAILG
                     GIRLGGGKGSVTKAVLGTLIILIISNGLLRLATPAGSSRVILATILLLAATLDIRWFK
                     NRHKAVQELYVSPSYLELPRSPPTGPASPFALNDRLRNVETIGLGEVESPEDVILDRD
                     DNLYCGSRHGEIIRFFAPDYKRREVYARIGGQPLGMAFDAGGTLYVCVGGMGLYRVKP
                     DRSVEKVTDETNRSLFSVVDDSRLRLADDLDIAPDGRIYFSEATIRYEMHEWPTDSLE
                     GRGNGRIICYDPRTDKTHTVLRHLIFPNGVVLASDGQSIIFAESWNCSIKRHWFAGEK
                     EGKTETVIASLPGYPDNINVASDGNYWLALMGMRSPAFDLAMRKPAFRRRMSKRLPGD
                     EWLAPNINTGCIVKFNEKGEILDALWDLGGENHPMITSMREHKGHLYIGGISNNRIGR
                     VKLEGADPEFVSSGRYNV"
     gene            6154..7248
                     /locus_tag="VGM46_07835"
     CDS             6154..7248
                     /locus_tag="VGM46_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192543.1"
                     /translation="MFNAVSSLISSLFDRGSSLSVPILDGALKPNNMIENATVVTERA
                     GIEDMALGPDGVLHLACGKDVLKLSDKGEFTVAASFDRPVTALAFTAAGEIAVGLGNA
                     VAVGSRIMDSADGKPFVAVNALSPTADGGLLITDGSTARPYAQWSHDLLEAGRTGRLL
                     RYDAASGRAVTLASGLGYAFGAMADARGRLVVSESWRHRLCVVEGGRTREAVPALPGY
                     PSRMSPTGDGGFWLTLFACRTQLVEFVLQESDYRREMMRTIDPQYWVAPALSSGNDYL
                     EPLQGGSVKQMGILKPWAPPRSYGLAVRFDAEFRPLYSVHSRTGGRNHGIVAAVQRGD
                     DLFLLSKGAGRVLRLSASQIQSAWAKGTNE"
     gene            7245..8735
                     /locus_tag="VGM46_07840"
     CDS             7245..8735
                     /locus_tag="VGM46_07840"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEY4192544.1"
                     /translation="MSAILELRQLTKIYARAAAVENIDFSLEKGEVHALLGENGAGKS
                     TLTKMIAGVVAPTSGTILLDGKEIALRTPSEALSSGIAMVFQETSLVPSMTVAQNLFL
                     GSESFFNRVRGINIAAQQFMQSLSFQVDPTVQVGSLGAAKKQMVEIARAMLLKARIIV
                     FDEPTATLTPEEKSHFFELVNTLKERGVSVIFISHALEEALSISDRITILRDGKLVLT
                     DRTANFDRARIIQAMVGRTLSQELYGTRKTSVRPAGRRVLSVSNLRVGNVVKNNSLSV
                     FAGQITGVFGLVGSGRTETFKVVAGAIKRDYANGGEVFLEGKRVRYRVPAQGVRDGVA
                     YITEDRKIEGFFETMSIKSNIFIGLLAKLRGKFWLKGREVDTVGDKWVKSLQVRAVSK
                     GARVIELSGGNQQKAVIAKSLVQDPELIIFDEPTRGVDVGAIAEIHQIINRLADEGKA
                     VVVISSYLPEVMLLSDRLLVARQGKVVEEFSPIEATEEKLMYAAVH"
     gene            complement(8848..9597)
                     /locus_tag="VGM46_07845"
     CDS             complement(8848..9597)
                     /locus_tag="VGM46_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY4192545.1"
                     /translation="MRLNGKTALVTGAASGFGVEIAATFAREGAKVIIMDLNGEGAET
                     TAKTIGAAACAIKGDVTKAADIQAAVDAAVAFGGKLDIVVNNAGWTHRNKPMLEVTEE
                     EFDRVYDINVRSIFHMSGAAVPLMQKQGGGVIINIGSVAGIRPRPGLTWYNSTKGAVN
                     LLSRSMAVELAPHNIRVNCVAPVMGATGLIEQFMGAPDTPENRAKFMATIPLGRFSTP
                     RDIANACLYLVSDDADFITGVVLEVDGGRTI"
     gene            complement(9624..11093)
                     /locus_tag="VGM46_07850"
     CDS             complement(9624..11093)
                     /locus_tag="VGM46_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY4192546.1"
                     /translation="MNDLIATAPVQSAKGVFIDNRWQPSKSGRLIDVYAPAEGAVFAQ
                     IAAGGAEDIDAAVKAARKAHEGAWGRLTATERGRLLSKLAQAILDNADELTLIEARDT
                     SKPLKQARADILTCARYFEFYGGAADKFHGETIPYMNGYFVATEREAHGVTGHIIPWN
                     YPAQMFARTIAPALATGNATVLKPAEDACLTPLRIAELAAEAGFPEGALNIVPGLGAE
                     AGAALISHPGIDFLSFTGSPEVGVMVQTEAAKNHITCTLELGGKSPHIVFADVDLEAA
                     LPSLVNAIVQNAGQTCSAGSRLLVEKKGYDRVIEAVAERFAGLRAGTPEMDLDLGALI
                     SPRQKKRVEAFFARAADDGIALLAEGGIADGVPQDGYFVAPKLYGPVPRANDLAREEV
                     FGPVLSVIPFEDEADAIGAANGTDFGLIAGVWTQDAKRATRVARKVRAGQVYINAYGA
                     GAGVELPFGGIRKSGHGREKGLAALHDFTTMKTIIVKHD"
BASE COUNT         1893 a         3652 c         3645 g         2033 t
ORIGIN      
        1 cccgagcgac aggtggatga agccgggaat ttcgctgtcg gcgaagagtt tcgacagggt
       61 cgtctccgtc gcccggatca gcagcatggt gcgatgaagg tcgagcagcc gcgaattgcc
      121 cgcgatgcca tgcgccgggg ccggccgcga catgtggcga cgccctgctg cggcgagggc
      181 aggccgccat aggtcgccca ggtggagccg gccagccagc cgcaatcgac cggcaggtcg
      241 atgccggaaa tggcggaggc ggcgggcgaa agcaggaagg caacggcctg tccgatctcg
      301 gaaggctgta ccagccgccc cagcggcgcg ttgccgacaa ggcgcgaaac gtcgcgccgc
      361 ccggcgtcga tctccgtttg caggccgggc gtcgccgtat agccgggcga aaccgaattg
      421 acccgcacgc cgtcgcggcc ccaagcggaa gccaggcagg cggtcatgga gatgaccgcc
      481 gccttggccg gggcatagga atgcagcggc atggagcgcg acccggcgac ggaggcgatg
      541 ttgacgatgg ccccgcccgt gccgcgcgcc gccatgcggc ggccgaaggc aaggcacgta
      601 acataggtgc cgcgctggtc gatatggacg atgcggtccc acaggccaag gtcgagttct
      661 tccggcggca gcggcggctg gatgacgccg gcgctggtga ccagcccggc gaccgcgccg
      721 aagcgcgcct cgatggcctg cgccgccgca tcgacgccgg ccgcgtcgcc cacgtcgatg
      781 gcgacgaaat caccgccgac atcgctggcc acctgcttgc cggcggcctc gctgacatcg
      841 gcgacgacga catggaggcc ggcatccgcg atgaccttgc agcaggcggc gccgatgccg
      901 ctggccccgc cggtgaccac gaccaccggg cggctcatgg tcggatatcc acggtggcgt
      961 agtgctggcc attcacgccg gaacggcgct ggaaagcgct ggcgaaacag ggcgcaggcg
     1021 gtgctgtcca aaacaggcgc ataaggtcct cccgaccaca aatattgact gttgaaccga
     1081 tagtcaaaaa tttgcttgtt tctgtcaagc cgcattttga cgatagccgc gcggccggca
     1141 ggcgatagga tgaggtgctt ttcccgataa aacccttgga tgcggcattt gtggcaaggg
     1201 atgggatttt tctgccgctg tcagacgacg gccgccccgg cacgatattt cggcaaaatg
     1261 ttgatttttg aatcgccagt caattttcat tgacacattg ccgccggttg atattgtttt
     1321 ggcaagcagc ggccgcttgg gaggagcgtc ctatgaactc ttatccattc atggtcgcaa
     1381 ttccagcccc gttgccgatc ccgcaaggga ttggccgtgt cctgccgccg cgctccgtcg
     1441 tggtgcagcc ggcgaatatg gcgggcaggg gtcggttttg cgtgcctgcc cgccaccgtt
     1501 cgtttgccgg acaggcggcg atttccggcg gacgccggcg ccagggcgtg gcgcgcggca
     1561 gggcgccaga acgtttcctt ataccggcca agcaaacaca gtctttgatc tggacactgt
     1621 tcttggagag actttttccc ggtggattgc cggcatgccg cgcgcatgca gggcggttcc
     1681 ggggagaatg ggtaggagcc atgccgatga gaccggcctt taccggcggg ccgccgatgg
     1741 cggcgtctgg ttccaccccg acacattcgg cgatccgaac cggaatcggt ggggcggacg
     1801 ggccatttct ttggtgcgac agggcaggca ggcgatgagt gcgagcgaac tcaagggcga
     1861 tggggcggag gctgcgggca aggccgggga cgatcgttcc gcgtccttcc tgaagggctt
     1921 cgccttcgac cagcagatga tcgtgctttt gctgtttgcg gtcattttcg cggctttcag
     1981 cattttcctg tccggctttt tcacggtggg caatcttttg acgctgatgc gcaccgtctc
     2041 ggtgctcggc atccttggcc tcggcatggc ggtggtggtg atcgggcgcg gcatcgacct
     2101 gtcgatgatc gcctcgctgg cggtgccgtc cgccgtggtg ctcatgcttg cctcgaagga
     2161 ctacggcatc gccggttcgc tgctttgcgg cgtggcgctg gcgctggcca tcggcgtttt
     2221 gaacggcttc ctgatcgcct atgcagaaat cccctcgctg ttcaccacgt tggcggtcgg
     2281 catcggcatc gccggcatcg gccagagtgg cctgttcgaa tatgaaatcg tgccgtggcc
     2341 gacgcagctt gcgagcatca gctggatcgg ccagggttcc ctgttcggcg taccctattc
     2401 ggtgctggcc ttcgtcgtgg cggccatcgt cgtcggcatc ttcatgcgca agacgcggct
     2461 tggcgccttc atctacgcca gcggcgacaa ccccaacacc gcccgcatcg ccggcatatc
     2521 ggtgcgtccg atcctcgtcc tgcaatatat gatcgccagc ctgatcgcga tgttcgcggg
     2581 gctggtgctt gccgcctcgt ccgcgggcat ggatacgcgc atcttcaacc tgacctggat
     2641 ctacgacgtc atcctggtcg tggtgctggg cggcatcggc ctttcgggcg ggcgcggcgg
     2701 cgtcctcaac gtggtgatcg gcacgctttt gatcggcacg atcgtcaacg gcatgaccat
     2761 catgaacgtg tcgttcgaaa tgcagaatct catcaagggc atggtcctgc tggccgccct
     2821 ttccatcgac agcatcgtca acccccgcaa cgaagagacc gcccagcagg gagacatcta
     2881 agcgcgccgc agggcgcgtt cgcagccaac atcaatcagg aggagaacat gatgggactg
     2941 aagaaaatcg ggcttgccat tgccatggcc gccaccgtgc tgaccggcgc ggcacaggcg
     3001 caaaacagca tgaacgaccc ggcgcgcccc ggctattacg aaaacctcaa gggcaggcgc
     3061 gtcgtcttcg tgccggtgtt catggggctg gacctcaccg agggctggtc caagatgatg
     3121 gcgcgccagg ctgagaagct gggctataaa tacgaggtcc gcaactccaa cttcaacacc
     3181 gccgccggcg cgcagacgct gacctcgctg atcaccgaaa agccggatgt catcgtggtg
     3241 caaaacccgg acgtgcagtc ctacgccaag ctgctggcca aggccgagaa ggccggcatc
     3301 cacgtcatcc agctcaacat gaagaccaat gtgctgacca ccggcttcgt cggcgccgat
     3361 gtcgaggagc ttggcgagtt gcaggccaag gaagtcgtcg cccattgcgg cacgggcact
     3421 tccggcaagg tgctggtgct ggccggtccg ccctccagcc cgttctccgc ctacatgctg
     3481 aagggctacg agaacattct caacgcggcg aaggacgtca aggtcgtctc ggtccagtcg
     3541 accggcgact atgaatccgc caaggcgaag tccatcaccc aggtggtgtt gcagcagcac
     3601 ccggacctgt gcggcgttct gggcgtgtgg gacaacgccg atgtcggcac cgctgccgcc
     3661 atcaaggaag ccggcaagag cgaccaggtg ttcgtgtcgt cgtcgggcgg cggcggcgaa
     3721 ctggcctgca agggcatcca ggaaggcatg tggcagcact atgtcagcta tgacgtgccc
     3781 ggacaggcgc gcgacctcaa taatctgatc ctcgcctcgt tgcaggacaa gaacccggtc
     3841 ggcaccacca agaccatcct ctacacgccg ctggtgatct acaacaagga caacctcaag
     3901 aaccagttct gctggacctt ggacacgatc cgctaagcca gacacctgaa ggcgaagggc
     3961 gccagccgcc cttcgccgca catgcccgga accggcccgc aaccggcccg caaccgcccc
     4021 gaactcgcct ttcgtccacg caaagcagac ggcgttcaat gtccaatacc ctcaccaaat
     4081 tacgctaccg cctctggccc gaccatgtgc tgggcgagct tctgcaaaag cagtggatgg
     4141 aaacggccgt gccgctgctg gtgctgctgc tgaccatcgc cttcttcagc tcgcgcatcg
     4201 acaatttcat ttcggtggcc aacctgtccg acacgctgcg gcaggccagc gaactggggt
     4261 tcgtcgtgct cggcatgtcg ctggtgctga tcgtcggcgg catcgacctt tccgtcgcct
     4321 cggtctttgc gctgtgcaac ctgaccgcgc tctattgcat caacgtgctc ggcctgccga
     4381 cgccggcggc catcgtgctg acgctgctgc tgggcgcggt gctcggcgcc gtcaacggcg
     4441 tgctgatcgg ctacatgcgc atgcgcgcct tcctcaccac catggtgacg ttgatcgtct
     4501 acaaggcgat ccacgatttg ctgaacttca agctggccgt ggcgatctct tcggtggtgc
     4561 cggacgtgcc cgcctgggat tatctgggcg gcggcgattt tctttcgatc ccgatggtgg
     4621 tgtgggtgtt cgcggccatc gccatcgcca gccacatctt cctcacccgg cttcgccacg
     4681 gctggtatgt catggccatc ggcggcaacc gccgctcggc ctataatatc ggcctgccgg
     4741 tcaaccgcgt cgtggccatg tcctatgtgg cgtccggcgt gttcgccgcc atctcggcca
     4801 tcttctatgc ctcgcgcctg gccagccccg gcgcggatac cggcaagggc ctcgaaatca
     4861 ccgtgctcac cgccgccatt ctgggcggca tccggctcgg cggcggcaag ggctcggtca
     4921 ccaaggcggt gctcggcacg ctcatcatcc tcatcatctc caacggcctg ctgcggctgg
     4981 cgacgccggc cggctcctcg cgcgtcatcc tggcgacgat cctgctgctg gccgccacgc
     5041 tcgacatccg ctggttcaag aatcgccaca aggcggtcca ggaactctat gtcagcccat
     5101 cctatctgga attgccgcgg tcgccgccga ccggccctgc ctcgcccttc gcgctcaacg
     5161 acaggctgcg caatgtcgag accatcgggc ttggcgaggt ggaatcgccc gaggacgtca
     5221 tcctcgaccg agacgacaat ctctattgcg gttcccgcca cggcgaaatc atccgcttct
     5281 tcgcccccga ctacaagcgc cgcgaagtct atgcccgcat cggcggccag ccgctcggca
     5341 tggccttcga cgccggcggc acgctctatg tctgcgtcgg cggcatgggc ctctaccgcg
     5401 tcaagcccga ccgctcggtc gagaaggtga ccgacgagac caaccgttcg ctgttttcgg
     5461 tggtggacga cagccgcctg cgccttgccg acgacctcga catcgcgcct gacgggcgca
     5521 tctacttctc cgaggcgacg atccgctacg agatgcacga atggccgacc gacagcctgg
     5581 aagggcgcgg caacggccgc atcatctgct acgatccgcg caccgacaag acgcatacgg
     5641 tgctgcgcca cctgatcttc ccgaacggcg tcgtgctggc ctcggacgga caatccatca
     5701 tcttcgccga aagctggaac tgctcgatca agcgccactg gttcgccggc gaaaaggaag
     5761 gcaagaccga aaccgtcatc gccagcctgc ccggctaccc ggacaacatc aatgtcgcct
     5821 ctgacggcaa ttactggctg gcgctgatgg gcatgcgcag ccccgccttc gaccttgcca
     5881 tgcgcaagcc ggctttccgt cgccgcatgt cgaagcggct gccgggcgac gaatggctgg
     5941 cgcccaacat caacaccggt tgcatcgtca agttcaacga aaagggcgaa atcctcgacg
     6001 cgttgtggga tctgggcggc gagaaccacc cgatgatcac ctcgatgcgc gagcacaagg
     6061 gccatctcta cataggcggc atttccaaca accgcatcgg cagggtcaag ctcgaagggg
     6121 ccgatccgga attcgtatcg agcgggcgct acaatgttta atgccgtttc gtctctgatc
     6181 tcatcgctgt tcgaccgcgg ttcgagcctt tcggtaccga tcctcgacgg cgcgctgaag
     6241 cccaacaaca tgatcgagaa cgccacggtg gttaccgagc gggcgggcat cgaggacatg
     6301 gcgctcggcc cggatggcgt gctgcatctt gcctgcggca aggatgtgct gaagctgtcg
     6361 gacaagggcg aattcaccgt cgccgccagt ttcgaccggc cggtgaccgc gcttgccttc
     6421 accgccgccg gcgagatagc agtcggcctg ggcaatgcgg tggccgtcgg ctcccgcatc
     6481 atggacagcg ccgacggcaa gcccttcgtc gcggtcaacg cgctgtcgcc caccgccgat
     6541 ggcgggctgc tgatcacgga cggctccacc gcgcggccct atgcgcaatg gagccatgac
     6601 cttctggagg cgggccggac cggccggctg ctgcgctacg acgcggcctc cgggcgcgcc
     6661 gtaacgcttg cttccggcct tggctacgcc ttcggcgcga tggccgacgc gcgcggacgc
     6721 ctggtggtca gcgaaagctg gcggcaccgg ctttgcgtgg tcgagggcgg gcgcacgcgc
     6781 gaggcggtgc cggcgctgcc cggctatccg tcgcggatgt cgccgaccgg ggatggcggt
     6841 ttctggctga ccttgtttgc ctgccgcacg cagcttgtcg aattcgtgtt gcaggaaagc
     6901 gactaccgcc gcgagatgat gcgcaccatc gacccgcaat attgggtggc gccggcgctc
     6961 agttccggca acgactatct ggaaccgctg caaggcggca gcgtcaagca gatgggtatc
     7021 ctcaagccct gggcgccccc gcgttcctat ggcctggcgg tgcgcttcga tgccgagttc
     7081 aggccgcttt attcggtgca tagccgcacc ggcgggcgca accacggcat tgtcgccgcc
     7141 gtgcagcgcg gcgacgacct gttcctgctt tcgaagggcg ccggccgcgt gctgcggctt
     7201 tcggcttcgc aaatccagtc ggcctgggcc aaggggacga acgaatgagc gccatcctcg
     7261 aactgcgcca gcttaccaag atctatgcgc gcgccgccgc cgtcgagaac atcgactttt
     7321 cgctggaaaa gggtgaggtg cacgcgctgc tcggcgaaaa cggcgccggc aagtcgacgc
     7381 tcaccaagat gatcgccgga gtggtcgcgc cgacctccgg caccatcctg ctcgacggta
     7441 aggagattgc gctgcgcacg ccgtccgagg cgctgtcgtc cggcatcgcc atggtgttcc
     7501 aggaaaccag cctggttcct tccatgacag tggcgcaaaa cctgttcctg ggcagcgaaa
     7561 gcttcttcaa ccgcgtgcgc ggcatcaaca tcgccgcgca gcagttcatg cagtcgctga
     7621 gcttccaggt cgatccgacc gtgcaggtag gctcgctcgg cgcggccaag aagcagatgg
     7681 tggagatcgc gcgcgccatg ctgctcaagg cgcgcatcat cgtcttcgac gagccgaccg
     7741 ccacgctgac gccggaggag aagagccact tcttcgaact ggtcaataca ctgaaggaac
     7801 gcggcgtctc ggtcatcttc atctcgcatg cgctggagga ggcgttgtcg atttccgacc
     7861 gcatcacgat cctgcgcgac ggcaagctgg tgctgaccga caggaccgcc aatttcgacc
     7921 gcgcccgcat catccaggcc atggtcggcc gcacgctgtc gcaggaactc tacggcaccc
     7981 gcaagaccag cgttcgcccc gccggacggc gcgtgctcag cgtcagcaat ttacgcgtcg
     8041 gcaatgtggt gaagaacaat tcgctgtcgg tcttcgccgg ccagatcacc ggcgttttcg
     8101 ggctggtcgg ctccggccgc accgagacgt tcaaggtggt ggccggcgcg atcaagcgcg
     8161 actacgccaa tggcggcgag gtgttcctcg aaggcaagcg cgtgcgctac cgcgtgccgg
     8221 cgcagggcgt gcgcgacggc gtcgcctaca tcaccgagga ccgcaagatc gaaggcttct
     8281 tcgagaccat gtcgatcaag tccaacatct tcatcggcct gctcgccaag ctgcgcggca
     8341 agttctggct gaagggccgc gaggtggata cggtcggcga caaatgggtg aagtcgctgc
     8401 aagtgcgcgc cgtcagcaag ggcgcgcgcg tcatcgaact gtcgggcggc aaccagcaga
     8461 aggcggtcat cgccaaatcg ctggtgcagg acccggaact catcatcttc gacgagccga
     8521 cgcgcggcgt cgatgtcggg gccatcgccg aaatccacca gatcatcaac cggctggccg
     8581 acgagggcaa ggcggtggtg gtgatctcgt cctacctgcc ggaagtgatg ctgctttccg
     8641 accgcctgct ggttgcccgg cagggcaagg tggtggagga gttctcgcct atcgaggcga
     8701 cggaggaaaa gctgatgtat gcggcggtgc actgacagga ccattccgcg ccacgggctg
     8761 gcgtccggct tcgtggcgac agcctcccaa ggcgacaaaa acccctggat cgacgatccg
     8821 ggggtttttg tttcgaccgc ttgccggtca gatggtgcgg ccgccatcca cttccaggac
     8881 gacgccggtg atgaagtccg cgtcgtccga taccagatag aggcaggcat tggcgatgtc
     8941 gcgcggcgtc gagaagcggc cgagcgggat ggtcgccatg aatttggcgc ggttttccgg
     9001 cgtgtcgggc gcgcccatga actgttcgat caggccggtc gcgcccatca ccggggcaac
     9061 gcagttgacg cggatattgt gcggggcaag ctcgaccgcc atggagcgcg acagaaggtt
     9121 caccgcgccc ttggtggaat tgtaccaggt caggccgggc cgcgggcgaa tgccggccac
     9181 cgagccgata ttgatgatga cgccgccgcc ctgcttctgc atcagcggca ccgccgcgcc
     9241 cgacatgtgg aagatcgagc gcacgttgat gtcatagacg cggtcgaact cttcctcggt
     9301 gacttccagc atcggcttgt tgcggtgggt ccagccggca ttgttgacga cgatgtcgag
     9361 cttgccaccg aaggcgacgg cagcgtcgac cgctgcctgg atgtcggcgg ccttggtcac
     9421 gtcgcccttg atggcgcagg cggcggcgcc gatcgtttta gcggtagtct cggccccctc
     9481 gccgttcagg tccatgatga ttacctttgc accctcgcgc gcgaaggtcg ccgcgatctc
     9541 gacgccgaag ccggatgcgg ctcccgtcac cagtgccgtt ttgccgttca gtctcatggt
     9601 cgtctccttg cttgcgatgg ccgtcagtcg tgcttgacga tgatggtttt catggtggtg
     9661 aagtcgtgca gggcagccag ccccttttcg cggccatggc cggatttgcg gatgccgccg
     9721 aagggcagtt cgacgccggc gcccgcgcca taggcgttga tgtagacctg gccggcgcgc
     9781 accttgcggg cgacgcgcgt ggcgcgcttg gcgtcctgcg tccacacgcc ggcgatcagg
     9841 ccgaaatccg tgccgttggc cgcgccgatc gcatcggcct cgtcctcgaa gggaatgacc
     9901 gacaggaccg ggccgaacac ttcctcgcgc gccaggtcgt tggcgcgcgg caccggcccg
     9961 tacagcttgg gcgccacgaa ataaccgtcc tgcggcacgc catccgcgat gccgccttcg
    10021 gccagcagcg caatgccgtc gtcggccgcc ctggcgaaga aggcttcgac gcgcttcttc
    10081 tggcgcgggc tgatgagcgc gccgaggtcg aggtccattt ccggcgtgcc ggcgcgaagg
    10141 ccggcgaagc gttcggccac cgcctcgatc acgcggtcgt agcctttctt ttccaccagc
    10201 aggcgcgagc cggccgaaca ggtctggccg gcattctgca cgatggcgtt gaccaggctg
    10261 ggcagcgccg cctcaaggtc gacgtcggcg aagacgatgt gcggcgattt tccgcccagt
    10321 tccagcgtgc aggtaatgtg gtttttggcg gcttccgtct gcaccatcac gcccacttcc
    10381 gggcttccgg tgaaggacag gaagtcgatg cccggatgcg agatcagggc agcgcccgct
    10441 tccgcgccaa ggcccggcac gatgttcagc gcgccttcgg gaaagcccgc ttcggcggcc
    10501 agttcggcga tgcgcaacgg cgtcaggcag gcgtcctcgg caggtttcag caccgtggcg
    10561 ttgccggtgg cgagcgccgg cgcgatggtg cgcgcaaaca tctgcgccgg atagttccag
    10621 gggatgatat ggccggtcac gccatgcgcc tcgcgctcgg tggcgacgaa atagccgttc
    10681 atgtagggga tcgtctcgcc gtggaacttg tcggccgcgc cgccatagaa ctcgaaatag
    10741 cgcgcgcagg tgagaatgtc ggcgcgcgcc tgcttcaggg gtttgctggt gtcgcgcgcc
    10801 tcgatgagcg tcaattcgtc ggcattgtcg aggatcgcct gcgcgagttt cgacaggagg
    10861 cggccgcgct ccgtcgccgt gagccggccc caggcacctt catgggcctt gcgcgcggcc
    10921 ttcaccgccg cgtcgatgtc ttcggcgccg ccggcggcga tctgcgcgaa cactgcgcct
    10981 tcggccggcg catagacgtc gatgaggcgg cccgacttcg acggctgcca tcggttgtcg
    11041 atgaacacgc ccttggccga ctgcacgggc gcggttgcga ttagatcgtt catgtctctg
    11101 cttcctcaca tttgcctggt cccgcccgcc gcgcatgcgg tgggcggacg gctggcgtcg
    11161 tcagaattcg gccttcgctt ccatatggat gcggccgccg ctctcagtcc aataatcatg
    11221 ccc
//
LOCUS       DASYCS010000245        11365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1366879,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000245 DASYCS010000000
VERSION     DASYCS010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 11365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11365
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1366879"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..766)
                     /gene="cmk"
                     /locus_tag="VGM46_07855"
     CDS             complement(122..766)
                     /gene="cmk"
                     /locus_tag="VGM46_07855"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006325316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEY4192547.1"
                     /translation="MSLTIAIDGPAGAGKGTLARRLSDHYRLNLLDTGLTYRAVAHAL
                     LRLGLPLDNVSAAETAARQVDLAKLDRAVLSAHAVGEAASKVAVFPTVRRILVEKQRE
                     FAKTPPGAVLDGRDIGTVVCPDADIKLYVTASAEVRAERRLAEIESIGGTANFAEILA
                     DIVRRDERDMGRADSPLKPAADAHLLDTSEMAIEAAFLAAMAVIDDVLAKRNKA"
     gene            complement(780..1628)
                     /locus_tag="VGM46_07860"
     CDS             complement(780..1628)
                     /locus_tag="VGM46_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007196306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HEY4192548.1"
                     /translation="MAAPLKRVMMRSAASAMRRANAAQWHYGPGFDPQKAAAQHQELV
                     KLVSDAGAKVEWLTDEDDGLADSVFTFDPSLMTDHGAIILSMGKALRLPEPGLHEAAY
                     TRMGIPILGRIEAPGQVEGGDTVWLDKQTLIVGRGVRTNQEGIQQLSNLLSPKGISVY
                     GFDLPLWQGEEACLHLMSVISPLADDLALVYSPLLPAPFYQMLKAKGIRLVEGDAEEF
                     RVSNGLSLNVLPTDQRQVIAIAGFPKTKAAMEAAGCTVSTFEADALCVACEGGPTCLT
                     RPVLRS"
     gene            1910..2707
                     /locus_tag="VGM46_07865"
     CDS             1910..2707
                     /locus_tag="VGM46_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006325323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4192549.1"
                     /translation="MPADKHGAQKDAPEAIHIENLHKKFGELHVLKGVSLSARDGEVI
                     AIIGGSGSGKSTLLRCINCLENPTSGIVRVNGEEIRMKADSHGNTQPADRRQIERIRS
                     KLGMVFQSFNLWSHMTLIENVIEVPVHVLGVKRDEAIAQAEKLLDRVGLAEKRDVYPA
                     YLSGGQQQRAAIARALAIQPRVMLFDEPTSALDPELVGEVLKVIGDLAREGRTMVLVT
                     HEMKFAREVATHVVYLYNGLVEEEGPPEAIFGAPKSERLKQFIRNIG"
     gene            2772..3569
                     /locus_tag="VGM46_07870"
     CDS             2772..3569
                     /locus_tag="VGM46_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010913030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY4192550.1"
                     /translation="MKNFLKTTAVAAVFALAGAFSASAEQTKVGFSPEAYPPFYAQDS
                     SGNWTGWEVDITMAICAEAKLDCVLTPIPWDGLIPALKTKKIDTIMNSLSITAERKKE
                     IDFSDKYYNTPTVIVGPKSEKFDATPEGLKGKILGVQVSTVHAAYAKKHFADSASEIK
                     EYQTQDEANQDLVAGRIDATQADGIALDAFLKSDQGKECCEVKGTVAPDLEVLGPGVG
                     AGVRKGDTELAGKINAAIKAIRANGKYDEIGKKYFDFDIYGDETPAN"
     gene            3777..4484
                     /locus_tag="VGM46_07875"
     CDS             3777..4484
                     /locus_tag="VGM46_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008129155.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY4192551.1"
                     /translation="MALLSLSPPGWGANLLVGLLKSIEIALGAFGLGLLIGICGAYGK
                     LYGGPVVRDLFEVYTTVVRAVPELVLILLLYYAGTDLINQMLAVFGHAPIDINGLVAG
                     IGVLGVVQGAYSTEVLRGAILAIPQGQVEAARAYGMPPRLMLKRITLPAMLPFALPGL
                     ANLWLISTKDTALLAIVGFSELTQTTRLAAGATKAYLTFFVAAGVLYLLLSLVSNFFI
                     ARLERWARRGMPSLRDA"
     gene            4487..5293
                     /locus_tag="VGM46_07880"
     CDS             4487..5293
                     /locus_tag="VGM46_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4192552.1"
                     /translation="MAIDGIIRPAARPSPWLQPHRIVLMLIALGLVLAAALFMRWDWL
                     PTYLPLAVQGIWRTLWLLVVTSLLGIALAIPLGLAQAAGPKFFSAPALAFCTVIRGTP
                     LLLQLWLLYYGLGSLFPQFPWIRDSFLWPYLRQAWPYGVLSLTLSYAGYEGEVMRGAF
                     AGVPRGQLEAARAFGMSRWTTFRRIWLPQALYRALPTLAGETVLQLKATPLVATITMV
                     DVYSVASRVRQETFITYEPLLLLAVVYMAVTGILVFVFRRIEARMPSRVG"
     gene            5310..6308
                     /locus_tag="VGM46_07885"
     CDS             5310..6308
                     /locus_tag="VGM46_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEY4192553.1"
                     /translation="MHLSLEQATGLCRMALLGAGAGDEAARSLAAACVAAQAEGNDGV
                     GLSHLIDYLEALDAGRIDGKAEPVITRPALAVYLSDARAGIAHTGFDRTVDDLAKAAR
                     LFGVAIFSQKNAYTCGALGYFTGRLAAKGLVSFAATNGPALLAGGGSAKPVYSTNPMS
                     FAAPAADGVPLLIDQASSAAAYVAIRKAAQDGEQIPAGWALDADGNPTADPAAAMKGA
                     LLAFGGNRGANIALMVEVLAAGLSGANWSLDAPSFTDGVKSPATGLFVLAIEPKLLDP
                     DFEQRMAGQIERLRGHGVHVPGRAGKAMADRAAAEGLTVPNEVVQRISEFAARHSA"
     gene            6404..7378
                     /locus_tag="VGM46_07890"
     CDS             6404..7378
                     /locus_tag="VGM46_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HEY4192554.1"
                     /translation="MTEILQTPKLVVVFGGSGFVGRHVVRALAKRGYRIRVGCRRPDL
                     AGHLQPLGNVGQIQPVQANLRVRWSVDRAVEGADHVVNLVGILHQSGRQKFTTVHQFG
                     ARAVAEAARAVGAGLTHISALGADVNSASAYARTKALGERAVFETLPDAVVLRPSIVF
                     GPEDEFFNRFAAMARFSPVLPLIGGGATKLQPVYASDIAEAVARSVEGKVAGGRIYEL
                     GGPSVLTFKESMQELLAVTERKRLLVPVPWWIANLQASVLGLLPKPLLTKDQVIQLRT
                     HNVVSPEAVQQGRTFAGLGIQPQALGTILPSYLWRFRAAGQFQRKPAA"
     gene            complement(7444..8250)
                     /locus_tag="VGM46_07895"
     CDS             complement(7444..8250)
                     /locus_tag="VGM46_07895"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010659405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEY4192555.1"
                     /translation="METHTILEALLLGIIEGLTEFIPVSSTGHILLAGHFLGFESTGK
                     VFEILIQLGAILAILGVYFRRLWKMLIDFPHDRLTRHFVLGIVLAFLPAAVIGALAHD
                     FIKTVLFESPKLICTMLLIGGVVLLWVDRMDLKPVYRDVERFPLRRYIEIGLFQCLAM
                     IPGTSRSGATIVGAMLLGVEKRAAAEFSFFLAMPTMVGAFAFDLIKNRHLLTGSDFSL
                     IAIGFVAAFISALFVVRFLLDYISRHGYSLFGWWRLIVGGVGLVALLIWG"
     gene            8403..9095
                     /locus_tag="VGM46_07900"
     CDS             8403..9095
                     /locus_tag="VGM46_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006016645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY4192556.1"
                     /translation="MLTLFHHPMFASCRFVRLAFGEYGEELALIEEKPWTRRKEFLAL
                     NPAGTLPILLAEGDVPIVGATVIAEYIDETRGVLKRDKRLFVEDPIERAEIRRLTDWY
                     LNKTESEVTRHLVRERVLKPVMPETAGGGSPDSAAIRAARANIRQHMKYTNWLAGTRP
                     WLAGNKVTYADLAAASALSVLDYLGEIDWREHHAAREWYTRVKSRPSFRPLLADRVRG
                     LTPVSHYADLDF"
     gene            9076..10248
                     /gene="queG"
                     /locus_tag="VGM46_07905"
     CDS             9076..10248
                     /gene="queG"
                     /locus_tag="VGM46_07905"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEY4192557.1"
                     /translation="MRTSISEAAKLRALIDREARGAGFDAVAVTTPDAIPLAPARLAE
                     FVDAGFHGSMGWIAETLLRRSDPSTLWPEVRSIIVLAMNYAPDSDPRDILARKSSGAI
                     SVYAQNRDYHDVMKGRLKEIAGKIVARAGGDVKVFVDTAPVMEKPLAGAAGLGWQGKH
                     TNLVSREHGSWLFLGTIFTTAALEPGAAEADHCGSCRACLDACPTNAFPAPYRLDARR
                     CISYLTIENKGPIPAEFRQAIGNRIYGCDDCLAACPWNKFAHAASEAKLCARDDLRAP
                     ALAGLLALDDAAFRSFFSGSPVKRIGRDRFLRNVLIAAGNSSDPALVPSVRTLMGDGS
                     PLVRGAAVWALSQLVPAGEFTDSAKAGLLSEHDDEVRGEWLAAMRAVREIEMETPA"
     gene            10245..11153
                     /locus_tag="VGM46_07910"
     CDS             10245..11153
                     /locus_tag="VGM46_07910"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006205352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY4192558.1"
                     /translation="MSLGRIFIFGAGYSAKAFAKAASATGAKMVGTTRSPEKFDALRQ
                     AGLQPLLFDGALTLEIEGALAETTHLIISTAPDEAGDPVLNAARKAIVNSMPSLAWIG
                     YLSTVGVYGDHGGDWVDESAECRPVSRRSVMRVAAEQEWLALGRQVERPVAVLRLSGI
                     YGPGRNAFVNLQNGTAKRLVKRDQVFNRIHRDDIAGALCQLAGQHRGGVFNITDDLPA
                     PPQDVVAYAAKLMGVAPPPEIPFEMAQLSPMARSFYGENKRVANKAIKEAGYPLRFPD
                     YRSAFERMWTDGNWAEGEARSPMKRS"
BASE COUNT         2035 a         3633 c         3592 g         2105 t
ORIGIN      
        1 aagccctggg ccgctcttta agcggcaatt ccggcgcatg aagaatgctg gcaggctggt
       61 tcgtgcccga tatgggtatt ttcgtcgctt ccggcaacgg aaacgcgaga gaaaacccgg
      121 atcaggcctt gtttctcttg gccagcacgt cgtcgatgac agccatggcc gcgagaaacg
      181 cggcttctat agccatttcg ctggtatcaa gcaagtgcgc gtctgccgcc ggcttcaagg
      241 gcgagtcggc gcggcccatg tcgcgctcgt cgcggcgcac gatatcggcg aggatctcgg
      301 cgaagttggc ggtgccgccg atgctctcga tctcggccag ccggcgctcc gcgcgcacct
      361 cggcgctggc cgtcacatag agtttgatgt ccgcatcggg acatacgacc gtgccgatgt
      421 cgcgcccgtc aagcaccgcg ccgggtggcg tcttggcaaa ctcgcgctgc ttttcgacca
      481 ggatgcgccg cacggtcggg aacaccgcga cctttgaagc cgcttcgccg accgcatgcg
      541 ccgacagcac cgcgcggtcg agctttgcca gatcgacctg gcgtgcggcg gtctcggccg
      601 ccgagacatt gtcgagcggc aggccaagcc gcagaagcgc atgggcaacg gcgcgatagg
      661 tgagcccggt atcgagcagg ttcagccggt agtggtcgga aagccgccgc gccagcgtgc
      721 ccttgccggc gccggcaggg ccatctatgg cgatggtcag gctcatgccc ccaacgcctt
      781 catgagcgca ataccggccg ggtcaggcag gtcggcccgc cctcgcaggc cacgcacagc
      841 gcatccgcct cgaaggtcga gacggtgcat ccggccgcct ccatcgccgc cttggtcttg
      901 ggaaatccgg cgatggcgat cacctgccgc tggtccgtcg gcaggacgtt gaggctgagg
      961 ccgttcgata cgcggaattc ctcggcatcg ccctcgacca gccgaatgcc cttcgccttc
     1021 agcatctgat aaaagggcgc cggcaaaagc ggggaataga ccagcgccag atcgtcggcc
     1081 agcgggctga tcaccgacat caggtgaagg caggcctctt cgccctgcca cagcggcagg
     1141 tcgaagccgt aaacggaaat gccctttggc gaaagcaggt tggaaagctg ctggatgcct
     1201 tcctgattgg tgcgcacgcc gcgcccgacg ataagcgtct gcttgtccag ccacacggta
     1261 tcgccgccct ccacctggcc cggcgcttcg atgcggccga gaatggggat gcccatgcgc
     1321 gtataggcgg cttcatgcag gccgggttcc ggcagccgca gcgccttgcc catcgacagg
     1381 atgatggcgc catggtcggt catcagcgac gggtcgaagg taaagacgga atcggcaagg
     1441 ccgtcatctt cgtccgtcag ccattccacc ttagccccgg catccgacac cagcttgacc
     1501 aattcctgat gctgggccgc cgccttttgc ggatcgaacc ccggaccata atgccattgc
     1561 gcggcattgg cgcggcgcat ggcgctggcc gccgatcgca tcatgacccg ttttaagggg
     1621 gctgccatcg actgcgatcc gtaagacttt tccaccgcgt tctccctgaa agtttctggc
     1681 tcaagcattc cttggctcaa taaccattgc agcaggccac gatcaatggt ccaaatacag
     1741 tggttgacgc atacaagcgg aacggtccat cttggaaaga aaacaccggc caggggtggt
     1801 cgccagagtt gtccaggggt ggtcaaaggg tggtaatggg taaaagattc caggcgtcgg
     1861 cggtttgtcc cctttcaggg accgggctac ggtgattgcg cctggcgcga tgccggccga
     1921 caagcacggt gcgcagaagg atgcgcccga agccattcat attgaaaacc tgcacaagaa
     1981 attcggtgaa ttgcatgtgc tgaagggtgt ttcgctgtcg gcgcgcgacg gcgaggttat
     2041 cgccatcatc ggcggttccg gttccggcaa gtcgaccttg ctgcgctgca tcaactgcct
     2101 ggaaaacccg accagcggca tcgttcgcgt caatggcgaa gaaatccgca tgaaggcgga
     2161 cagccacggc aacacccagc cggccgaccg caggcagatc gaacgcatac gctccaagct
     2221 cggcatggtc ttccagagct tcaacctgtg gagccacatg acgctgatcg agaacgtcat
     2281 cgaggttccg gtccatgttc tgggcgtcaa gcgcgacgaa gccattgcgc aggcggaaaa
     2341 actgctcgac cgcgtcggcc tggccgaaaa gcgcgatgtc tatcccgcct atctttccgg
     2401 cggccagcag cagcgcgcgg ccatcgcgcg cgcgctcgcc atccagccgc gcgtcatgct
     2461 tttcgacgaa ccgacatcgg cgctcgatcc cgaactggtc ggcgaggtgc tgaaggtcat
     2521 cggcgatctt gcccgcgaag gccgcaccat ggttctggtc acgcatgaga tgaagtttgc
     2581 ccgcgaggtc gcgacgcatg tcgtctatct ctataacggg cttgtcgagg aggagggtcc
     2641 gccggaggcg atcttcggcg cgccgaaatc ggaacggctg aagcagttta ttcgaaatat
     2701 cggataggcc gggactaaat gaatgggcct gtactaaacg aatgggaacg tcacaaatgg
     2761 gaggaataac gatgaagaat tttctgaaaa caacggcggt cgccgccgta tttgcgctgg
     2821 ctggggcgtt ttctgccagc gccgagcaga ccaaggtcgg cttttcgccg gaggcctatc
     2881 cgcccttcta tgcgcaggat tcgtccggca actggaccgg ctgggaggtc gacatcacca
     2941 tggcgatctg cgcggaagcc aagctcgatt gcgtgctgac gccgatcccg tgggatggcc
     3001 tgatcccggc gctgaagacc aagaagatcg atacgatcat gaattcgctg tcgatcaccg
     3061 ccgagcgcaa gaaggagatc gacttctccg acaagtacta caacacgccg accgtcatcg
     3121 tcggcccgaa atccgagaag ttcgacgcca cgccggaagg gctgaagggc aagattctag
     3181 gcgtgcaggt ttccaccgtg catgccgctt acgccaagaa gcattttgcc gacagcgcct
     3241 cggagatcaa ggaataccag acgcaggacg aagccaacca ggacctggtc gcgggccgca
     3301 tcgacgccac gcaggctgac ggcatcgcgc tcgacgcctt cctgaagtcc gatcagggca
     3361 aggaatgctg cgaggtaaaa ggcacggttg cgcccgacct cgaagtgctc ggccccggcg
     3421 tcggagcggg agtgcgcaag ggtgacaccg aactggccgg caagatcaac gccgccatca
     3481 aggcgatccg cgccaacggc aaatacgacg aaatcggcaa gaaatatttc gatttcgata
     3541 tctatggcga cgaaaccccc gccaactgat ctcattcgaa cacggccggc cgccttgctt
     3601 ttccggcagg gcggctggct gctcgatccc ggagcggctt aattcggcag gaggccgctt
     3661 cgttgattaa ttcgcattcg atctacaaaa gcctcaccgc gtttttttga tccggtgcca
     3721 taccgcgagg aattgacctg tacgatgttc ttgccgctgc cttcgcaagc agcacgctgg
     3781 cgcttctgtc gctgtcgccg cccggatggg gcgcgaacct tctggtcggc ctcctcaagt
     3841 cgatcgagat cgcgctgggc gcattcggcc ttggcctgct catcggcatt tgcggcgcct
     3901 atggcaagct ttatggcggt cccgtggtcc gtgacctgtt cgaggtctac acgaccgtgg
     3961 ttcgcgccgt acccgaactg gtcctgatcc tgctgctcta ttatgccggc accgatctca
     4021 tcaaccagat gctggccgtg ttcggccatg cgccgatcga catcaatggg cttgtcgcag
     4081 gcatcggcgt gctgggcgtc gtccaaggtg cttattccac cgaggtgctg cgcggcgcga
     4141 tccttgccat tccgcaaggt caggtcgagg cggcgcgcgc ctacggcatg ccgccccggc
     4201 tgatgctgaa gcgcattacc ttgccggcga tgctgccttt cgcacttccc ggtcttgcca
     4261 atctctggct gatttcgacc aaggataccg cgcttctggc gatcgtcggc ttcagcgaac
     4321 tgacccagac cacccgtctg gccgccggcg cgaccaaggc ctatctcacc ttcttcgtcg
     4381 ccgccggcgt cctttatctc ttgttgtcgc tggtctcgaa cttcttcatt gcccgtctcg
     4441 aacggtgggc gcggcgcggc atgccctcgc tcagggacgc atgacgatgg cgatcgatgg
     4501 catcatccgc cctgccgcac gcccgtcgcc gtggttgcag ccgcaccgca tcgtcctgat
     4561 gctgattgca ctcggccttg tcttggctgc ggcgctgttc atgcgctggg actggctgcc
     4621 cacctacctg ccgcttgccg tgcaaggcat ctggcgtacg ctttggcttt tggtggtcac
     4681 gtcgctgctt ggcatcgcgc tcgccattcc gcttggcctc gcccaggcgg ccggtccgaa
     4741 attcttcagc gccccggcgc tggcgttctg caccgtcatt cgcggcacgc cgcttctgct
     4801 gcaactatgg ctgctttatt acgggctagg ctccctgttt ccgcaatttc cctggataag
     4861 ggacagtttc ctgtggcctt atctgcgcca ggcatggccc tatggcgtgt tgtcgctgac
     4921 gctctcttat gccggctatg agggcgaggt gatgcgtggg gcctttgccg gcgtgccacg
     4981 cggccagctt gaggcggcgc gcgccttcgg catgagccgc tggacgacgt tccggcgcat
     5041 ctggctgccg caggcgctct atcgggcgct gccgacgctg gccggtgaaa ccgtgctgca
     5101 actgaaggca acgccgctgg tggccaccat taccatggtg gatgtctatt cggtggcgtc
     5161 gcgcgtgcgg caggagacgt tcatcaccta cgaacccttg ctgctgctgg ccgtggtcta
     5221 tatggcggtc accggcattc tggtgttcgt tttccgccgg atcgaagcgc gaatgccaag
     5281 cagggtaggc taaccccggg atcggcacga tgcatctcag tctagagcag gcgaccggcc
     5341 tgtgccgcat ggctttgctc ggcgccggcg ctggcgatga ggcggcgcgc tcgcttgccg
     5401 ccgcctgcgt cgcggcgcaa gccgaaggca atgatggcgt cggcctgtcg cacctgatcg
     5461 actatctcga agcgctggac gccggccgca tcgacggcaa ggccgagccg gttatcaccc
     5521 ggcctgccct ggcggtctat ctgtccgatg cgcgggccgg catcgcacat acgggtttcg
     5581 accggacggt cgacgacctc gccaaggcgg caaggctgtt cggcgtcgcc attttctcac
     5641 agaagaatgc ttatacctgc ggcgcgctcg gctatttcac cgggcggctg gccgccaaag
     5701 gtcttgtctc gttcgccgcc accaatggtc ctgccttgct ggcgggcggt ggctcggcca
     5761 agccggtcta cagcaccaac ccgatgtctt ttgccgcgcc tgccgccgat ggcgtccccc
     5821 tgcttatcga tcaggcctcc agcgccgccg cctatgtcgc catccgcaag gcggcgcagg
     5881 acggcgaaca gattcccgcc ggctgggcgc tcgacgccga cggcaacccg accgccgatc
     5941 cggcggcggc gatgaagggc gcgctgctgg ccttcggcgg caatcgcggc gccaatatcg
     6001 ccctgatggt cgaggttctg gcggcaggcc tttccggcgc caactggtcg cttgacgcgc
     6061 cgtccttcac ggatggcgtg aaaagtcccg ccaccggcct tttcgtcctc gccatcgaac
     6121 cgaaactgct cgatccggat tttgaacagc gcatggccgg ccagatagag cggttgcgcg
     6181 gccatggcgt gcatgtcccc ggtcgcgccg gcaaggccat ggcggaccgc gcggctgcgg
     6241 aaggcctgac cgtgcccaat gaggtggtgc agcgcatctc cgaatttgcc gccaggcatt
     6301 ccgcgtaaga ccccttcacg ctgcggcgac aaccgtgctt caccctttgc ctttgccgaa
     6361 aaaccgatta tgaaacgcca cagccaagcc tgcggagcca gccatgaccg aaatcctgca
     6421 aacgccaaaa ctcgtcgtcg ttttcggcgg ttcgggcttt gtcggccgcc atgtcgtgcg
     6481 ggcgttggcc aagcgcggct accgcattcg tgtcggctgc cgcaggcctg atcttgccgg
     6541 ccatttgcag ccgctcggca atgtcggcca gatccagccg gtgcaggcca atcttcgcgt
     6601 ccgctggtcc gtcgaccgcg ccgtcgaggg agccgaccat gtcgtcaatc tggtcggcat
     6661 cctgcaccag agcgggcggc agaaattcac caccgtccat cagttcggcg cgcgcgccgt
     6721 ggccgaagcc gcccgtgccg tcggcgccgg cctgacccat atttctgcgc tgggcgccga
     6781 tgttaattcg gcatcggcct atgcgcgcac caaggcgctt ggcgaaaggg cggtattcga
     6841 aacccttcct gacgctgtcg tcctgcgccc ctcgatcgtt ttcggaccgg aggatgaatt
     6901 cttcaaccgt ttcgccgcca tggcgcgttt ttcgccggtg ctgccgctga tcggcggcgg
     6961 cgcgacgaag ctccagccgg tttacgcctc cgacattgcc gaagcggtcg cgcgttccgt
     7021 ggaaggcaag gtcgcgggcg gccggattta cgagcttggc ggaccgagcg tgctgacctt
     7081 caaggaaagc atgcaggagc ttctggcggt caccgagcgc aagcgcctgc tggtgccggt
     7141 gccgtggtgg atcgccaacc ttcaggcatc ggtcctcggc ctcttgccaa agccgttgct
     7201 gacgaaggac caggtcatcc agttgcggac ccacaatgtc gtctcgcccg aggcggtgca
     7261 gcagggccgc acctttgccg gacttggcat ccagccgcag gcgctcggca ccatcctgcc
     7321 cagctatctc tggcgcttcc gcgccgccgg ccagttccag cgcaagcccg ccgcctgacg
     7381 gttcacgctt tcgggaaaat gtaggccggg caggaaaacg caggccggga cgtttccggt
     7441 ccgtcagccc cagataagca gcgcgacaag cccgacgccg ccgacaatca gccgccacca
     7501 gccaaacagc gaatagccgt ggcgcgagat gtagtcgagc aggaagcgca cgacgaacag
     7561 cgccgagatg aacgccgcca cgaagccgat ggcgatgagc gagaagtccg atcccgtcag
     7621 caaatgcctg ttcttgatca ggtcgaaggc gaatgccccg accatcgtcg gcatggcgag
     7681 gaagaacgaa aactccgccg cggcccgctt ttcgacgcca agcagcatgg caccgacaat
     7741 ggtggcgccc gaacgcgagg tgccgggaat catcgccagg cactggaaca ggccgatctc
     7801 gatatagcgc cgcaggggga accgctcgac atcgcggtag accggcttca ggtccatgcg
     7861 gtcgacccac agcagcacca cgccgccaat caacagcatg gtgcagatca gcttgggcga
     7921 ttcgaacagc accgtcttga tgaagtcgtg cgccagcgcg ccgatgacgg ccgccggcag
     7981 gaacgccagc acgatgccga ggacgaaatg ccgcgtcagc cggtcgtggg gaaaatcgat
     8041 gagcatcttc cacagccgac gaaaataaac gccgaggata gccaggatag cgccgagctg
     8101 gatgaggatt tcaaatacct tgcccgtcga ttcgaagccg agaaaatgcc ccgcaagcag
     8161 gatatggcct gtcgaggaca ccggaatgaa ttcggtcagt ccctcgatga taccgagcag
     8221 gagggcttca aggatggtat gggtttccat ggggtctcat tcacggcttg cttgcacggc
     8281 ttgcttgtca tggcgcggaa ctaaccctat agctcgcaga atatgaccgc cctgtgaatc
     8341 gggctgccaa gacttatcgg cgcgggcggc gattcgccag agcgcattta tatcgctgga
     8401 ccatgctgac gcttttccat cacccgatgt tcgcttcctg tcgctttgtc cggcttgcct
     8461 ttggcgagta tggcgaagaa ctggccttga tcgaggaaaa gccgtggacg cggcgcaagg
     8521 aattcctggc gctcaacccg gcgggaacgc tgccgatcct gcttgccgag ggcgatgtgc
     8581 cgatcgtcgg cgccaccgtc atcgccgaat atatcgacga gacgcgcggc gttttgaagc
     8641 gcgacaagcg cctgttcgtc gaagacccga tcgagcgcgc cgaaatacgc cggctgaccg
     8701 actggtatct caacaagacg gagagcgagg ttacccgcca tctggtgcgc gagcgcgtgc
     8761 tgaagccggt aatgccggaa acggctggcg gcggctcgcc cgattcggcg gcgatccggg
     8821 cggcgcgcgc caatatccgc cagcacatga aatacaccaa ctggctggcc ggcacccggc
     8881 cgtggctcgc cggcaacaag gtcacctacg ccgatctggc cgccgcttcc gctctgtcgg
     8941 tgctcgacta tctgggcgag atcgactggc gcgagcatca cgccgcgcgc gaatggtata
     9001 cgcgggtcaa gtcgcgccct tccttccggc cgctgctggc agaccgcgtg cgcggcctca
     9061 ccccggtgtc gcattatgcg gacctcgatt tctgaggcgg caaagcttcg cgcgctgatc
     9121 gaccgcgagg cgcggggcgc gggcttcgat gccgtcgccg tcaccacgcc ggacgccata
     9181 ccgctcgccc ccgcaagact tgccgaattc gtcgacgccg gctttcacgg ttcgatgggc
     9241 tggatcgccg aaaccctgct gcgccgcagc gatccttcga cactgtggcc ggaggtgcgc
     9301 tcgatcatcg tgttggcgat gaattacgcg ccggacagcg atccgcgcga catactggcg
     9361 cgaaagagca gcggcgccat ctccgtctat gcgcaaaacc gcgactatca cgatgttatg
     9421 aaaggccggc tgaaggagat cgccggcaag atcgtcgcca gggcgggcgg cgacgtgaag
     9481 gtgttcgtcg atacggcgcc ggtaatggaa aagccgctgg caggcgccgc cggcctcggt
     9541 tggcaaggca agcacaccaa tctggtcagc cgcgaacatg gctcatggct gttcctgggc
     9601 acgatcttca ctacggcggc tctggagcct ggcgcggctg aagccgacca ttgcggctcc
     9661 tgccgcgcct gcctggatgc ctgcccgacc aacgcttttc ccgcacccta caggctcgat
     9721 gcgcggcgct gcatctccta tctcaccatc gagaacaagg ggccgatacc cgccgaattc
     9781 cggcaggcca tcggcaaccg catctatggc tgcgacgact gcctcgcggc ctgtccgtgg
     9841 aacaagttcg cccatgccgc ctccgaggcg aagctttgcg cgcgcgacga tctgcgcgcg
     9901 ccggcgttgg ccgggctgct ggcgctggac gatgcggcgt ttcgcagctt cttctccggc
     9961 tcgccggtca agcgcatcgg ccgcgaccgc ttcctgcgca atgtgctgat tgcagcgggc
    10021 aattcgagcg atccggcgct tgttccatcg gttcggaccc tgatgggcga tgggtcgcca
    10081 ttggtgcgcg gcgcggctgt ctgggcgttg tcgcagcttg tgccggccgg cgaattcacc
    10141 gatagcgcga aggccgggct tttgagcgaa catgacgatg aagttcgcgg cgaatggctt
    10201 gccgccatgc gggcggtacg ggaaattgaa atggagacgc ctgcatgagc ttgggcagaa
    10261 tattcatctt cggcgcaggc tattcggcaa aagcgtttgc aaaagcagca tccgcgaccg
    10321 gcgcgaagat ggtcggcacg acacgctctc cagaaaaatt cgatgcgttg cggcaggcgg
    10381 gcttgcagcc cttgctgttc gacggcgcgc tgacgctgga aatcgaaggg gcgctcgctg
    10441 aaaccacgca cctcatcatt tccacggcgc ccgacgaagc cggcgatccg gtgctgaacg
    10501 ccgcgcgcaa agcgattgtg aattccatgc cgtcgctggc atggatcggc tatctgtcga
    10561 cggtcggcgt ctatggcgac catggcggcg actgggtgga cgagagcgcc gaatgccgcc
    10621 cggtgtctcg ccgctccgtc atgcgggttg cggccgagca ggaatggctg gcgcttggcc
    10681 ggcaggtcga gcggcccgtc gcagtcctgc gtctttccgg catctatggg ccgggccgca
    10741 acgccttcgt caatttgcaa aatggcacag ccaagcggct ggtcaagcgg gatcaggtgt
    10801 tcaaccggat ccaccgcgac gatattgccg gcgcgctttg ccaactcgcc ggacagcatc
    10861 gcgggggcgt cttcaacatt accgacgatc ttccagcgcc gccgcaggat gtcgtcgcct
    10921 acgccgcaaa gctgatgggc gtcgcgccgc caccggaaat cccgttcgag atggcgcaat
    10981 tgtcgccgat ggcgcgctcc ttttacggcg agaacaagcg agtggccaac aaggcgatca
    11041 aggaggccgg ctacccgttg cgtttccccg attatcgctc ggccttcgag cgaatgtgga
    11101 ccgatggcaa ttgggccgag ggtgaagccc gcagcccgat gaagcgatcg tgatcgccgg
    11161 acgcgacatt gccggtgatc gcgatatttc ggcgcgttaa acttccgcta accacggcgg
    11221 ctcaaggtcc ggcaaaaagg tcaaaggtct gagatggaag aaatgctgct tcgttcccgt
    11281 ggtggtaacc gcctgttgac tgctgcgctc gccgccatca cggtgctggc gcttggcgtg
    11341 cagccttccg gcgcggtcga gcggg
//
LOCUS       DASYCS010000246         6845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2815593,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000246 DASYCS010000000
VERSION     DASYCS010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 6845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6845
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2815593"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /gene="hemE"
                     /locus_tag="VGM46_07915"
     CDS             complement(<1..722)
                     /gene="hemE"
                     /locus_tag="VGM46_07915"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEY4192559.1"
                     /translation="MPANRTMLNVLKGETVFPPPVWMMRQAGRYLPEYREARKQAGSF
                     LDLCYNPDFAVKVTLQPIERFGFDASILFSDILVVPHALGRDLRFEEGRGPMMTPITA
                     AEIAGLDGEAFHVNLEPVYETVRRLRKELPGETTLIGFCGAPWTVATYMIAGHGTPDQ
                     APTRLFAYREPAAFARLLAILADHSAAYLIRQIEAGADVVQIFDSWSGILDEANFEAF
                     CVKPVAEIVRKVRLVYPDVPIIG"
     gene            1125..1943
                     /locus_tag="VGM46_07920"
     CDS             1125..1943
                     /locus_tag="VGM46_07920"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006205316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HEY4192560.1"
                     /translation="MNKPQSFFHLHLISDATGETLLAAGRAASAQYKDARAIEHIYPL
                     VRTEKQLMKVFDDIEEEPGIILYTLVDEKLARSIDERCAAMGLPCVSVLEPVLSVFQS
                     YLGTPAGRRVGAQHVLDADYFRRIDALNFTMEHDDGQLPLNMEDADIVLIGISRTSKT
                     PTSIYLANRGIKTANIPIVLGVPVPESLVSAKTPLVVGLIATAERISHVRQSRALGVT
                     NGYEANEYIDRATITEELAYARQICTRHGWPMIDVSRRSIEETAAAIVALRGKR"
     gene            1991..2590
                     /locus_tag="VGM46_07925"
     CDS             1991..2590
                     /locus_tag="VGM46_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008935028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf-like protein"
                     /protein_id="HEY4192561.1"
                     /translation="MTEKIILASASPFRKALLVNAGVTVEAVPPDVDERALEAPLQGS
                     GVSPEDVAAILAEAKAVNVSEKKPGALVLGADQTLSLGDEVFHKPADMEGARRHLLAL
                     SGKTHQLNSAAVLARDGVVLWRHVGIANLTMRKLDPAFIGRHLAGVGEKALGSVGAYQ
                     IEGEGIQLFEEVDGDYFTIVGLPLLAVLAELRKLGAIDG"
     gene            2583..3428
                     /locus_tag="VGM46_07930"
     CDS             2583..3428
                     /locus_tag="VGM46_07930"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006205318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEY4192562.1"
                     /translation="MADPFKKAFVTGHPIAHSRSPKIHAYWLAKYGIEGSYEAIDVAP
                     EKFAEFLAGLQGQGLRGGNVTIPHKETAFALVRRRDEAAEQIGAVNTLWFEDGLLCGG
                     NTDAHGFAANLDEHAPGWAQNGAAVVLGAGGAARAVIHALKGRGVKDIRIVNRTLSRA
                     RELAQRFGAGVSAHETAATGELLAGAGLLVNTTSLGMHGNEAVAADPARLPGHAIVTD
                     IVYVPLETPLLATARLRGLKTVDGLGMLLHQAAPGFEHWFGVRPQVTPELRALVVADL
                     AGEKK"
     gene            3425..4030
                     /gene="coaE"
                     /locus_tag="VGM46_07935"
     CDS             3425..4030
                     /gene="coaE"
                     /locus_tag="VGM46_07935"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873253.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEY4192563.1"
                     /translation="MIVLGLTGSIGMGKSTAAKMFADAGVPVHDSDEAVHRLYAGAAA
                     PLVEAAFPGTVVDGVVDRSLLGGQVIGNPAALKRLAAIVHPLVRTDADAFLARNRAKN
                     APLAVLDIPLLFETGGRVRVDKVVVVSAPAKAQRARVLARPGMSEEKFASILSQQVPD
                     AEKRAQADYVIDTGGDFDATRAAVLKIIAELTGDKGASPAS"
     gene            4110..4814
                     /gene="dnaQ"
                     /locus_tag="VGM46_07940"
     CDS             4110..4814
                     /gene="dnaQ"
                     /locus_tag="VGM46_07940"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEY4192564.1"
                     /translation="MREIIFDTETTGLDSREDRIIELGGIELVNRFPTGRTFHKFINP
                     QGRVIHPDAQAVHGISAADLADKPGFHEIAEEFLLFIDGAKLVAHNANFDIGFLNFEF
                     GRLGHPAIDALLVVDTLALARRKHPMGPNSLDALCRRYGIDNTHRTKHGALLDSELLA
                     EVYIELIGGKQAALGLDGVVPAAGRREAGQVAIVVQARPAPLAPRLSEADRAAHAEMV
                     GTLGEKALWLKAGIAS"
     gene            complement(4968..5465)
                     /gene="secB"
                     /locus_tag="VGM46_07945"
     CDS             complement(4968..5465)
                     /gene="secB"
                     /locus_tag="VGM46_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEY4192565.1"
                     /translation="MAENNDAATGAGSNGNGAQPSLNVLAQYVKDLSFESPGAPNSLR
                     GRDKAPGIAINVNVNANPLSDNQFDVNLTLNAKASFDKEVLFNVELVYGGVFAVSGFP
                     QEHMLPILFIECPRLLFPFARQIIAEATRNGGFPPLMLDPIDFAQMFQQKMAEDQAAS
                     KVQVS"
     gene            complement(5575..6078)
                     /locus_tag="VGM46_07950"
     CDS             complement(5575..6078)
                     /locus_tag="VGM46_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HEY4192566.1"
                     /translation="MRISTFPILFLALPFLEIAGFVIVGRHIGALATVGLVLASTVAG
                     SMLLRHQGLGVMAKVRAEMDAQRDPSRQLAHGAMIVLAAVLLIVPGFLTDIFALLLLL
                     PPVRDFAWSRLKGRVAVAGSFGTGGFGGGFRSRERGKVIDLDDEDYSRSGGRSDPDSP
                     WRRLKDK"
     gene            6282..>6845
                     /locus_tag="VGM46_07955"
     CDS             6282..>6845
                     /locus_tag="VGM46_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011423414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44/TimA family putative adaptor protein"
                     /protein_id="HEY4192567.1"
                     /translation="MGLFDFGTIFFVIAAVVIFFQLRNVLGRRTGSERQPFDPYSSRT
                     TAKDASKAPENVISLPRKRAPGEPPVDVYAAIDAFAKPDTDLNKGLRAIKDNDPTFEP
                     KGFVDGARMAYEMIVMAYADGDRKTLKNLLSKEVYDGFVAAIGEREAKSEKIQSSFVG
                     IDKADIVSAEMKGGEAHLTLRIVSELIS"
BASE COUNT         1327 a         2166 c         2123 g         1229 t
ORIGIN      
        1 ccgatgatcg gaacgtcagg gtagaccagc ctgactttgc gtacgatctc ggccaccggc
       61 ttgacgcaaa acgcttcgaa attggcctcg tccagaatcc cggaccagga atcgaagatc
      121 tgcacaacgt cggcgcccgc ctcgatctgc cggataaggt aggccgccga atgatcggcc
      181 agtatggcaa gcagccgcgc gaatgccgcc ggttcacgat aggcaaacag ccgcgtcggc
      241 gcctggtccg gcgtgccgtg cccggcgatc atataggtgg cgacggtcca cggcgccccg
      301 caaaagccga tcagcgtcgt ttcaccgggc aattccttgc gcagccgccg caccgtctca
      361 tagaccggtt cgagattcac gtgaaacgct tcgccgtcca ggccggctat ttcagccgcc
      421 gtaatcggcg tcatcatcgg cccgcgccct tcctcgaaac gcaggtcgcg gcccagcgca
      481 tgcggcacga cgagaatgtc ggaaaacagg atcgaggcgt cgaagccgaa acgctcgatc
      541 ggctgtagcg tcaccttcac ggcaaagtcg ggattatagc aaaggtcgag aaatgacccg
      601 gcctgcttgc gcgcctcccg gtattccgga aggtagcggc ctgcctgacg catcatccag
      661 accggaggcg gaaaaaccgt ctcgcctttg agaacgttca gcatggtgcg atttgcaggc
      721 atcaagcccc tcattcacaa gcccctctat aattaaagat tctatcgaaa agagtcttct
      781 gattctaaga gtctgttggt tgtggtggtt tgcctctgtc cccaatccgc gccaaaaaag
      841 ccagacagca tattgtcgcg tgttcttttc tcacaatagg aaaacagtgg ggataagtgc
      901 agattcccca tctgaatcaa atcgctatga aattctccaa aaaaagaacc gttgtggacc
      961 aagcctgaag tgctaaatcc ttccccggac ttttccccag cggctcgaag atcgccgccc
     1021 gcacccaatt gtggacaagt ccgcaactga tacagtcgct cttcgccatc cttgccgaaa
     1081 gcccgtcttt ttccacatca acccacagcc cggccgtgaa ccctgtgaac aaaccccaaa
     1141 gcttcttcca cctgcatctg atttccgatg cgacgggcga gacgctgctt gccgccggcc
     1201 gcgccgcctc ggcgcaatat aaggacgcgc gcgccatcga gcatatctac ccgcttgtcc
     1261 gcaccgaaaa gcagctgatg aaggtgttcg acgacataga ggaagagccg ggcatcatcc
     1321 tctatacgct ggtcgacgag aagctcgccc gcagcatcga tgagcgctgc gctgccatgg
     1381 gcctgccttg cgtttcggtg ctggagccgg tgcttagcgt attccagtcc tatctcggca
     1441 cgccggccgg ccggcgagtc ggcgcccagc atgtgctcga cgccgattat ttccgccgca
     1501 tcgacgcgct gaacttcacc atggagcatg atgacggcca gttgccgctc aacatggaag
     1561 acgccgacat cgtgttgatt ggcatttcgc gcacctcgaa gacgccgacc tcgatctatc
     1621 tcgccaatcg cggcatcaag accgccaata tcccgatcgt gctcggcgtt ccggtgcctg
     1681 aaagcctggt cagcgccaag acgccgctgg tcgtcggcct gatcgccacg gcggagcgca
     1741 tctcgcatgt gcgccaaagc cgcgcgctcg gcgtcaccaa cggctacgag gccaatgaat
     1801 atatcgaccg cgcgaccatc accgaggaac tggcctatgc gcgccagatc tgcacgcgcc
     1861 acggctggcc gatgatcgat gtcagccgcc gctccatcga ggaaaccgcc gcagccatcg
     1921 ttgccctgcg cggcaagcgc tgaccgcttc ccacgcggcc ggccagccga atgaaataaa
     1981 acggaataaa atgacggaaa agatcatcct cgcttcagcc agcccgtttc gcaaggcgct
     2041 gctggtcaat gccggcgtca cggtggaagc ggtgcctccc gatgtcgatg aacgcgccct
     2101 tgaagcgccg ttgcagggca gcggcgtgtc gccggaagat gtcgccgcaa ttctggccga
     2161 agccaaggcc gtcaatgtca gcgagaagaa acccggcgca ttggtgcttg gcgcggacca
     2221 gacgctgtcg ctcggtgacg aggtgttcca caagcccgcc gacatggaag gcgcgcgccg
     2281 ccatcttctg gcgctgtcgg gcaagacgca ccagttgaac agcgccgccg tgctggcgcg
     2341 cgacggcgtc gtgttatggc ggcatgtcgg tatcgccaac ctgaccatgc gcaagctcga
     2401 tccggccttc atcggccgcc atctggccgg tgtcggcgag aaagccttgg gcagcgttgg
     2461 cgcctaccag atagaaggcg agggcatcca gctgttcgaa gaggtcgacg gcgactattt
     2521 caccatcgtt ggcctgccgc tgctggccgt gctggccgaa ttgcgcaagc tgggggcgat
     2581 cgatggctga tccgttcaag aaggccttcg tcaccggcca tcccatcgcc cattcccgtt
     2641 cgccgaaaat ccacgcttat tggctggcga aatatggcat cgaaggcagc tacgaagcaa
     2701 tcgacgtcgc gccggagaaa ttcgccgaat tcctggcggg cctgcaaggg caggggcttc
     2761 gcggcggcaa tgtcaccatt ccgcacaagg agacggcctt tgcgctggtc cggcggcgcg
     2821 acgaggcggc ggagcaaatc ggcgcggtca acacgctgtg gttcgaggac ggcctcctat
     2881 gcggcggcaa caccgacgcg cacggctttg ccgccaatct cgatgaacat gcgccgggct
     2941 gggcgcaaaa cggtgcggcg gtcgtgcttg gggcgggggg tgcggcgcgc gccgtcatcc
     3001 atgcgctgaa aggccgtggc gtgaaggata tccgcatcgt caaccgcacc ctgtcgcggg
     3061 cgcgcgagct tgcgcagcgc ttcggcgcgg gcgtctcggc gcatgaaacg gcggcgaccg
     3121 gtgaattgct ggccggtgcc ggcctgctcg tcaacacgac ctcgcttggc atgcacggca
     3181 atgaggctgt tgccgccgac ccggcacggt tgcccggcca cgccatcgtc accgacatcg
     3241 tctatgttcc gctggaaaca ccgcttttgg cgacagcgcg cctgcgcggg ttgaagaccg
     3301 tcgatggcct gggcatgttg ctgcatcagg ccgcccccgg cttcgagcac tggttcggcg
     3361 ttcgccctca ggtcacgccg gaactgcgcg cgctggtcgt tgccgacctg gcgggtgaaa
     3421 agaaatgatc gtgcttggcc tcaccggctc gatcggcatg ggcaagtcga cggcggcgaa
     3481 gatgtttgcc gatgccggcg tgccggtgca cgattccgac gaagccgtgc atcgcctcta
     3541 tgccggcgcg gccgcgccgc tggtcgaggc ggcgttcccg ggcacggtcg tcgatggcgt
     3601 cgtcgaccgc tcgcttctgg gcgggcaggt gatcggcaat ccggccgcgc ttaaaagact
     3661 agcggcgatc gtccatccgc tggtccgcac cgatgccgac gcctttctgg cgcgaaaccg
     3721 cgcgaagaat gcgccgcttg ccgtgctcga tatcccgcta ttgttcgaga cgggcggccg
     3781 cgttcgcgtc gacaaggtcg ttgtcgtcag cgcaccggca aaagcgcagc gcgcccgcgt
     3841 gctggcaagg ccggggatga gcgaggagaa gttcgcttcc atcctgtcgc agcaggtgcc
     3901 cgatgccgaa aagcgcgctc aggcggatta cgtaatcgac accggcggcg atttcgacgc
     3961 cacccgcgcg gcggttttga aaatcatcgc cgaactgacg ggggataagg gtgcctcgcc
     4021 ggcatcctga acttgcagcg ccggccgcca gtctccattt taagccgtcg gcgcaactgc
     4081 gtcgtcgccc atccgacgga gccggccact tgcgtgaaat catcttcgat accgaaacca
     4141 ccggcctcga ttcgcgcgaa gaccgcatca tcgagcttgg cggcatcgaa ctggtcaacc
     4201 gctttcccac cgggcgcacc ttccacaaat tcataaaccc gcaaggccgc gtcatccatc
     4261 ccgacgccca ggcggtgcat ggcatcagcg ccgccgacct agccgacaag ccgggctttc
     4321 acgaaattgc cgaggagttt cttctcttca tcgatggcgc gaagctggtc gcgcacaatg
     4381 ccaatttcga tatcggcttc ctcaatttcg agttcggacg gctcggccat ccggcgatcg
     4441 acgcgctgct ggtcgtcgat acgctggcgc tggcccgccg caagcacccg atggggccga
     4501 actcgctcga cgcgctgtgc cggcgctacg gcatcgacaa cacgcaccgc accaagcatg
     4561 gcgcgttgct cgattcggaa ttgctggctg aagtctatat cgagctgatc ggcggcaagc
     4621 aggcggcgct cggcctcgac ggcgtggtgc cggcggccgg ccgccgggaa gccgggcagg
     4681 tggccatcgt cgtccaggct cgcccggcgc cgctcgcgcc ccggttgagc gaggcggacc
     4741 gcgccgccca cgccgaaatg gtcggcacgc tgggagaaaa ggcattgtgg ctcaaggccg
     4801 gcatcgcatc atgaaaaacc cggcgcgagg ccgggtttaa tggtcgtctg cgccagtccg
     4861 gttttagagc atttacagca aaagcgtgga acggttttgc gtctggaaat gcagctgaaa
     4921 caaagagata gagcggtttt accgattcgg taaaaaccag aaccgcgcta gctgacctga
     4981 accttggaag cggcctgatc ctcggccatc ttctgctgga acatctgcgc gaaatcgatg
     5041 gggtcgagca tcagcggcgg gaagccgcca ttgcgcgtcg cctcggcgat gatctggcgg
     5101 gcgaacggga acagcaggcg cgggcattcg atgaacagga tcggcagcat atgttcctgc
     5161 gggaagccac tgaccgcgaa tacgccgcca tagaccagtt ccacgttgaa cagcacttcc
     5221 ttgtcgaagg aagccttggc gttcagcgtc aggttgacgt cgaactggtt gtccgagagc
     5281 gggttggcgt tgacattgac gttgatggcg atgccgggag ccttgtcgcg gccgcgcagc
     5341 gagttcggcg cgccggggct ttcgaacgac aaatccttga cgtactgggc aagcacgttg
     5401 agcgaaggct gcgcgccgtt tccattgctg ccggcgccgg tcgccgcgtc gttgttttcg
     5461 gccatgagcc ctgtcccctt gcctggaccg ttcaggcccg gattgaaatc gggcgttggc
     5521 taccatggcc ggcatgacaa gacaaggttt tacggcctgc ccgcaggctc gggcctactt
     5581 gtccttgagc cgtcgccagg gcgagtccgg atcggacctg ccgccgctcc tggaataatc
     5641 ttcgtcgtcg aggtcgatga ccttgccgcg ctcgcggctg cggaaaccgc caccgaagcc
     5701 gcccgtgccg aaactcccgg caacggccac gcggcccttc agccggctcc aggcgaaatc
     5761 gcgcaccggc ggcaacagca aaagcagcgc aaaaatatcg gtcaggaaac cgggcacgat
     5821 gagcagtacg gcggccagca cgatcatcgc gccatgggcg agctgccggc taggatcgcg
     5881 ctgcgcgtcc atttcggcgc gcaccttggc cattacgccc aacccttgat ggcgcagcag
     5941 catcgagccg gcaaccgtcg aggccagtac caggccgacg gtggccagcg ctccgatatg
     6001 gcggccgacg atgacgaagc cggctatttc cagaaatgga agcgcgagaa acaggatagg
     6061 aaaggtggag atacgcaaat ccggcccgat cggttcgagt ttggccaaca ggcgcaatcg
     6121 gcgctggcac acagcctgct agataggtat gcccgccttt gatttgaatg atggcgccgg
     6181 cagttctata tgcttagcct gttctatatg ctttgatggt ggaacgccat gctgcggcgt
     6241 ggacggccga tggggccgca acaggattga tggcgcaaga catgggactt ttcgacttcg
     6301 gcacgatttt ctttgtgatc gcggcggtgg tgatcttttt ccagctgcgc aacgtgcttg
     6361 gccgccgcac cggcagcgag cgccagcctt ttgatcccta ttcaagccgc acgaccgcca
     6421 aggatgcctc gaaggcaccg gaaaacgtca tctcgctgcc gcgcaagcgg gcgccgggcg
     6481 agccgccggt cgatgtctac gccgccatag acgccttcgc caagcccgac accgatctca
     6541 acaagggcct gcgcgccatc aaggacaatg acccgacttt cgagccgaag ggcttcgtcg
     6601 acggcgccag gatggcctat gagatgatcg tgatggccta tgccgacggc gaccgcaaga
     6661 cgttgaagaa cctgctgtcg aaggaggtct atgacggatt cgtcgccgcg atcggcgagc
     6721 gcgaggcgaa atcggaaaag atccagtcgt cctttgtcgg catcgacaag gccgatatcg
     6781 tttcggccga aatgaagggc ggcgaggccc accttacgct gcgcatcgtc agcgagctta
     6841 tttcg
//
LOCUS       DASYCS010000247        17231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_641435,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000247 DASYCS010000000
VERSION     DASYCS010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 17231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17231
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641435"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VGM46_07960"
     CDS             <1..459
                     /locus_tag="VGM46_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEY4192568.1"
                     /translation="EMFLERVNNHARANLLYDPSHFVLQQLDYLDYIDIYHERIKAFH
                     VKDAEFNPTGRQGVYGGFQNWVDRAGRFRSLGDGQVDFGAIFSKLTAYDFDSWAVLEW
                     ECALKHPEDGAREGAEFIKHHIIRVTDHAFDDFAGAGTDDAANRKMLGLA"
     gene            469..1644
                     /locus_tag="VGM46_07965"
     CDS             469..1644
                     /locus_tag="VGM46_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018431103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY4192569.1"
                     /translation="MAIEGSRQQAQAGRIRLGMVGGGQGAFIGAVHRIAARIDDQFEL
                     VAGALSSDAARAKASAEELGIAPERSYASYQEMARAEAARPDGIEAVSIVTPNNVHAP
                     AARAFLEAGIHVICDKPMTTTVKEAEALVELVKQTGKVFVLTHNYTGYPMMRQARAMV
                     AAGELGDIRVVQVEYPQDWLTERAELSGSKQAEWRTDPKRSGAGGSIGDIGTHAYNLA
                     AFVTGLTTDELLAQLTTFVPGRQLDDDVQIMLRYRGGARGMLWASQVAVGNENGLKLR
                     VYGSKGGLEWTQADPNYLWFTRFGEPKQLLTRAGAGAWPQAARVSRIPGGHPEGYLEG
                     FATIYAEAARAIRAARSGAAPDADVIYPTVQDGLDGMRFIEAAVASSQGGNVWTKVG"
     gene            1641..3191
                     /locus_tag="VGM46_07970"
     CDS             1641..3191
                     /locus_tag="VGM46_07970"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003506585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEY4192570.1"
                     /translation="MSPFADLPPVNAGVPLLEAVGLSKSFGPVQVLKDISLGVNAGEV
                     HAIIGENGAGKSTLMKLLSGHLQPTHGELHIGGETVALTGPVDAERRGIVLVHQEILL
                     APDLTVAQNVYLGRELHKPGLVGGIVVDDRAMNAGARQAVRDLGADIDPDVVVGRLSI
                     AQRQLIQIARVLLVPHRVVIFDEPTASLTPVETDALLKVITDIRAKGVGVFYISHRLP
                     EVKEIADKVTVLRDGRLVASHPASELEPVDMARLMVGRDVAKLYPDRQAETGRETVLE
                     VEDFSVPGYAQNASFKLRKGEILGFAGLVGAGRTELMEGIVGLRPGRGALRIGGKPAQ
                     FANVDASLKAGIAYLSEDRKGKGLLLTEDLRINLTLASLDRFQRGIQIDSARERAALD
                     KAIAEFDIRASRKDMLAGQLSGGNQQKLLLAKMMMTDPGIIIIDEPTRGIDIGTKEQI
                     YKFIAELSAQGRSIIVVSSEMQELIGICDRIVVMRGGRLVGEVAGEHMNENEIVVLAT
                     GVKSGEAA"
     gene            3202..4203
                     /locus_tag="VGM46_07975"
     CDS             3202..4203
                     /locus_tag="VGM46_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019444319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4192571.1"
                     /translation="MTDLAATNQKVRKSWKDIDLRAVAPFVALALLLVLGAFANPNFL
                     GIDNLLNVITRSAFIAIIAIGATFVIASGGLDLSVGSMVAFVASLMILFMNSGLIADS
                     ALMLFAAMGVAVLVGLACGLANGLVTTVGRIEPFIATLGTMGIYRGLTTWLSQGGAIT
                     VKSPEIQTLYRPAYFGAVLGVPVPIIVIAAVALIGAFILHRTRYGRHVLAVGSNEDVA
                     RYSGIPVDRVRTVTYIIQGLCVAVACLLYVPRLGSTSATTGLMWELQAITAVVVGGTA
                     LRGGVGRIWGTVCGAFILEIVGNIMLLSNFVSEYLIGAIQGAIIIIAMLVQRSLVRK"
     gene            4275..5225
                     /locus_tag="VGM46_07980"
     CDS             4275..5225
                     /locus_tag="VGM46_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007527748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY4192572.1"
                     /translation="MRKRMLGFAAVAMAALMSVSYAQDKKVTIGVSIPAADHGWTAGI
                     VFHAERVAKLLMQEHPGLNVIVKTSPDAASQANAVQDLAVQGIDALVILPSDPDPLVN
                     AIKEVKAKGAFVTLVDRAPSSNDDSVRDLYIAGNNPALGEVAGKYIKDTTPDAQVVII
                     RGLPIPIDQQRQDGFDKGIAGSGVKVLDRQFGNWNRDDAFKVMQDYLTKYPKIDVVWC
                     QDDDMAVGVLQAIEQAKRTDIQYVVAGAGSKDMVKKVMDGDKLVPVDVLYPPAMIGTA
                     MELTANNFYDQVPVRGSFILDATLVTKDNAKDFYFPDSPF"
     gene            5472..6041
                     /locus_tag="VGM46_07985"
     CDS             5472..6041
                     /locus_tag="VGM46_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011579496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEY4192573.1"
                     /translation="MHRTLFLVCSFLCSAMFAAHADTLSDLQGRYAIQPSSRVAFSVA
                     QVGGGGIDGVFQHFSGTFDLHPDNLARSSVNFSLQPGSVATGQSRVDDFLRSSAVFNV
                     AAYPVISFRSTAVRQQGSDHAVIEGMLTARGIAHPETFDATLVKRQGRTVAFHVVGQM
                     LRSPYGMDVGTPIYSNVARFDMVLDGQRK"
     gene            6156..6545
                     /locus_tag="VGM46_07990"
     CDS             6156..6545
                     /locus_tag="VGM46_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020012500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192574.1"
                     /translation="MRSLILAAVMMTAAMPLAYADDDYAGGAIKSMDTAKGEVLTDAK
                     GMTLYTFDKDAGGASNCYDACATKWPPLAAAAGAAVEGKYTLVDRKDGSKQWAYDGHP
                     LYLWQNDKKPGDVTGDGVGGVWHAAKE"
     gene            6575..7084
                     /locus_tag="VGM46_07995"
     CDS             6575..7084
                     /locus_tag="VGM46_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020012499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY4192575.1"
                     /translation="MSQVMDEIEECVPALRRYARALTHDPDRADDLVQDSLERAIRKR
                     GLWQPSGSVRSWVFRILLNVHRNELRRLRRAFTPVSLEALPGGEPAGLDVQPGRLALA
                     ETARAMQALPAEQREVLLLVAVEEMSYAEAAAVLSIPLGTLMSRLARARAALRHMTET
                     GAPKLRAVK"
     gene            7081..7854
                     /locus_tag="VGM46_08000"
     CDS             7081..7854
                     /locus_tag="VGM46_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008599207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEY4192576.1"
                     /translation="MSSEEHNITPHDLHAYADGLLDGPARAAVEAYLAQNPAAADEVE
                     GWKRQNETLRALYGHVAAEPVPAHLNVYRVERESRIKAMRWSRMAAAAVILLGLGTAA
                     GWYGRGSFAAGVPAQVALVDEAMEAHRVYSGEVAHPVEVWAGEKDHLQTWLSKRLART
                     LAVPDLKAAGLDLVGGRLLPAASGPAAQFMYEDDAGHRVTLYIIPAQEGRETSFRYAS
                     LDRLEAFFWTDETISCALVGDLPRARLQEIATQAYKQLG"
     gene            7894..8424
                     /locus_tag="VGM46_08005"
     CDS             7894..8424
                     /locus_tag="VGM46_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018011125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEY4192577.1"
                     /translation="MSIAFHWTIALLFFGQIALGYLTQATASDPRLQFDLYQWHKSFG
                     FLVLALALLRVLWALSGVWPRPLPGAARGEAAAGRAAHLLLLALTLAVPLTGWAIAST
                     SLLRIPSFMFDLVVIPDLPMARSDEMEAFWSGAHEWLAYGAGLLALVHAGAAIHHHLI
                     RRDATLRRMLGAGPRP"
     gene            complement(8498..9946)
                     /locus_tag="VGM46_08010"
     CDS             complement(8498..9946)
                     /locus_tag="VGM46_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007699118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEY4192578.1"
                     /translation="MTDSTMPSAPAQFATERDPRLRLIIPAIVAVAFFMENLDSTIIA
                     TAIPEMAKSLATTPIRLNLAITTYLLTLAVFIPVSGWFADRFGARRIFALALFTFTLG
                     SVLCGLANSFEMLLATRALQGLGGAMMTPVGRLILLRSFPRSQLVTAMLYMTLPAVMG
                     PVMGPLLGGVLTTYASWRWIFYVNVPFGCLGILLALRFVEDVRGEAPRRFDFVGFVMV
                     GAGVALLQFGLENIGRSVLPLPVVIAILLAAILLLVAFSRYARTIAAPAVDLTLFRFR
                     SFLVGTLAGGLCRVGLNGVPFLLPLMLQVGFHMSPVASGSLTFVSSLGALLIRPLLPS
                     MLRLFGFGAVLTGSAFAGSAAVAGFALLQPDTSAWLIGAYVALFGLIRAAQFMTSNTL
                     SYADLPPEKLSSATSLGGVLQQLSVSFGVSIGAMLLGLVAWENPALTPESFHTVFLFA
                     AIIPLLSIPGFFLLRPEDGVQVSGHHGRKSAS"
     gene            complement(10127..10543)
                     /locus_tag="VGM46_08015"
     CDS             complement(10127..10543)
                     /locus_tag="VGM46_08015"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEY4192579.1"
                     /translation="MTRHAVAAIGDIPDGGRLKVEVAGRAIVVFHVDGAFYALADKCP
                     HQGGPLSQGDQIGELRATAPGQHLYCRRNMVIRCPWHHWEFDIETGLSQIDPKRVRVR
                     SYETAVGDASPCEAGERLVATTFDVVSDEGLVYVIV"
     gene            complement(10540..11646)
                     /locus_tag="VGM46_08020"
     CDS             complement(10540..11646)
                     /locus_tag="VGM46_08020"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY4192580.1"
                     /translation="MNLHQPAATSQTPVTQKIIDCDVHPKLTSVNDLMPFLSERWKAH
                     LATYGLRYRMGYQDGNPYPKAAPASSRRDSWPDDGGLPGSDVGLLQSQLLDLYDMEFG
                     IMNPLWPTGQGVLGDDYSAALCRATNQWQLDLYNAPEPRLKCSICVPYENAELARAEI
                     EHFAGDRRFAQVLLLSRTAELLGQRRYWLIFEAAQAADLPVGIHVFGYSGQAITPAGW
                     PSYYMEEMTEHAPSTQAVLASMIFEGLFERFPRIRIVLLEAGLAWIPALGWRLDRIWA
                     RMRSEVPHVKRPPSEYLKQHLWVATQPMEEPENKRHLVDVLDWIGHDRVVFSSDYPHW
                     DFDDPRHSLPPHIDAKLKRAILHDNAAALYGTAR"
     gene            complement(11643..12725)
                     /locus_tag="VGM46_08025"
     CDS             complement(11643..12725)
                     /locus_tag="VGM46_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019998362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY4192581.1"
                     /translation="METATALLPKGSVNCDIHHPNPTMSQLMPYLADVWQDLIAARGI
                     GQLDSIAYPANAPLTCRPDWRDESGGLSNTPERLAREALDRPGAETGILNNLCGVHML
                     YDSYMAIAVTRALNDWTRFEWLDRHPGLRASIVAPLQDIEAAIAEVERCAADRRFVQI
                     LLPAAAHMPYGHRSYWPLYEAAARHGLPIGIHAGSAYHHPVTSLGWPNTVAEDYAAQS
                     QIMQTQLASFVSEGVFTQFPSLKVVLLESGISWLPAFLWRFGKFWKGLRFETPWVERP
                     PAEIIRDHVRLTTQPFDVPDDPHIVSRLIDHIGSDEMLLFSSDYPHWQFDGDAMLPAG
                     FDAAMVRKIAVENPAATYPRLSEVRP"
     gene            complement(12871..13812)
                     /locus_tag="VGM46_08030"
     CDS             complement(12871..13812)
                     /locus_tag="VGM46_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018426557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HEY4192582.1"
                     /translation="MRVIGFLLVHRFPMFSLAAAVDTMRTANHTLGRQFYRWVTISFD
                     GQPVIASNGMKLNVDHSLKDVPACDIVFVCAGTQIDVPEKAAVLSALRRWGRAGAALG
                     ALTLGSHILAQSGQLDGHRCTIHWENRAGFRESFPDIECSANVYEIDAKRYTSAGGTT
                     SMDLFLEIIRRDLGAPIADEVAGQFQHERVRQTSDRQRVGPGRDLATKSPVLRQVIEL
                     MADNLENPLSIEKLAKAAGLSARQMERLFAAEMKTTPGRYYIGLRLERARALLRQTPL
                     PILRVALDTGFSSQSYFAQSYRARFACLPSHERRPTT"
     gene            complement(14313..15836)
                     /locus_tag="VGM46_08035"
     CDS             complement(14313..15836)
                     /locus_tag="VGM46_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015917236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase (NADP(+))"
                     /protein_id="HEY4192583.1"
                     /translation="MTQAHTLHGKHLIAGQWVASEATFASDPAKGEAHRFSLGATAEI
                     DRAAKAAAEAFKVYGWTSRESRAAFLEAIAEAIDARGAAITAIGSAETGLPEARLEGE
                     RGRTTGQLRLFASHIRDGAYLDRRHDAALPERKPAARPGIKLVQRPIGPVGVFGASNF
                     PLAFSTAGGDTAAALAAGCPVVVKGHPGHPGTGEIVAEAILEAIKKTGQHPGVFALVQ
                     SNTNEAGEALVTHPLIKAVGFTGSLRGGRALFDLAARREEPIPFFGELGSVNPMFVLP
                     QAARARAAEIAKGWAGSLTMGVGQFCTNPGIVVIEDGEPADIFEQEARTALAGIAAQP
                     MLTTGIAEAFRRGAARIAASNAVRSAYEGAAQDRNAAPALFVTDGETFMANPELGEEV
                     FGPLGVVVRASDAGQMAAIAEGLHGQLTVTLQIDAGDTEQARGLVPILEQKAGRLLAN
                     GFPTGVEVVDAMMHGGPYPASTNTATTSVGTLAIRRFLRPVAYQNLPDALLPDDLAV"
     gene            complement(15847..16812)
                     /locus_tag="VGM46_08040"
     CDS             complement(15847..16812)
                     /locus_tag="VGM46_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006467507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY4192584.1"
                     /translation="MTATKPTSPAPISPAPISLAIVGVGKIARDQHVPSILGNPAFQL
                     AATASRSGAIDGVEAFDSIEALLGNRPDIQAVALCQPPQVRFEAAAKALRAGRHVLLE
                     KPPGATLSEVQALVEMASERRLALFATWHSRFAAAVEPARRWLADKTIRHVTVTWKED
                     VRRWHPGQAWIWQPGGLGVFDPGINALSIFTAILPRPMHLVRSNLEFPAGRAAPIAAQ
                     LAFEGEDGVTASADFDWRQTGPQTWDIEVATAEGRLVLSKGGAEMTIDEKPVVVEDDR
                     EYAGIYRRFAELVRSGEVDVDLAPLVHVADAFMLGERQTVEAFED"
     gene            complement(16799..>17231)
                     /locus_tag="VGM46_08045"
     CDS             complement(16799..>17231)
                     /locus_tag="VGM46_08045"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020051223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEY4192585.1"
                     /translation="IMVMKNCGPRGYPGMAEVGNMGLPPKVLRKGITDMVRISDARMS
                     GTAYGTVLLHVSPEAAVGGPLAIVRNGDMIEVDVAGRRLHLDVPEAEIAARLAAWTAP
                     MAVPESGYARLFATHVQGADTGADFDFLKGGRGKAVPRDSH"
BASE COUNT         2957 a         5508 c         5799 g         2967 t
ORIGIN      
        1 gagatgttcc tggagcgggt aaacaaccac gcgcgggcaa acctgctcta cgatccctcg
       61 cacttcgttt tgcagcagct cgactatctc gactacatcg acatctatca tgagcgcatc
      121 aaggcgtttc acgtcaagga cgccgagttc aacccgaccg ggcggcaggg cgtctatggc
      181 ggcttccaga actgggtgga ccgcgccggg cgcttccgct ctctgggcga cgggcaggtc
      241 gatttcggcg cgatcttttc caagctgacc gcctatgatt tcgacagctg ggcggtgctg
      301 gaatgggaat gcgcgctcaa gcacccggag gacggcgcgc gcgagggcgc ggagttcatc
      361 aagcatcaca tcatccgcgt caccgatcat gcgttcgatg attttgccgg cgccggcacc
      421 gacgatgcgg ccaaccgcaa gatgctcggg ctggcatagg gaggcgacat ggcgatcgag
      481 ggcagcaggc agcaagcaca ggccgggcgc atcaggcttg gcatggtcgg cggcgggcag
      541 ggcgcgttca tcggcgcggt tcaccgcatc gccgcgcgca tcgacgacca gttcgaactg
      601 gtggctggcg cgctgtcttc cgacgcggcg cgcgccaagg catccgccga ggaactgggc
      661 atcgcgcccg aacgctccta tgcgtcctat caggagatgg ccagggcgga agccgctcgg
      721 ccggacggca tcgaggcggt gtcgatcgtc acgcccaaca acgttcatgc gcccgccgcc
      781 cgcgcctttc tggaagcggg catccacgtc atctgcgaca agccgatgac caccacggtc
      841 aaggaagccg aggcgctggt cgagctggta aagcagaccg gcaaggtctt cgtcctgacc
      901 cacaattaca ccggctatcc gatgatgcgg caggcgcgcg ccatggtggc cgccggtgaa
      961 ctgggcgaca ttcgcgtcgt gcaggtcgag tacccgcagg actggctgac cgagcgcgcc
     1021 gagctttccg gctccaagca ggccgaatgg cgcaccgacc ccaaacgctc cggcgcgggc
     1081 ggctccatcg gcgatatcgg cacgcatgcc tataatctcg ccgccttcgt caccgggctg
     1141 acgaccgacg aattgttggc gcagctgacg accttcgtgc cgggccgcca gctggacgac
     1201 gatgtgcaga tcatgctgcg ctaccggggc ggcgcgcgcg gcatgctgtg ggcgagccag
     1261 gtggcggtcg gcaatgaaaa cggcctcaag ctgcgcgtct acggctccaa gggcgggctg
     1321 gaatggacgc aggccgaccc gaattacctg tggttcaccc gctttggcga gccgaagcag
     1381 ctgctcaccc gcgccggggc cggcgcatgg ccgcaggcag cgcgcgtcag ccgcataccg
     1441 ggcggacacc cggaaggcta tctggaaggc ttcgccacca tctatgccga ggcggcccgc
     1501 gccattcgcg ccgcgcgcag cggggccgcg cccgatgccg atgtgatcta tccgacggtg
     1561 caggacgggc tggacggaat gcgcttcatc gaggcggccg tcgcctcctc gcagggcggc
     1621 aatgtgtgga cgaaagtcgg atgagccctt tcgccgacct gccccccgtc aatgccggcg
     1681 tgcctttgct tgaagccgtc gggctgtcga agtcgttcgg cccggtgcag gtgctgaagg
     1741 acatcagcct tggcgtcaat gccggcgagg tgcatgccat catcggcgag aacggcgccg
     1801 gcaagtcgac gctgatgaag cttttgtccg gccatttgca gccgacgcat ggcgaactgc
     1861 atattggcgg cgagacggtg gcgctgaccg ggccggtcga cgcagagcgg cgcggcatcg
     1921 ttctggtgca tcaggaaatc ctgctcgcgc ccgacctgac ggtggcgcaa aacgtctatc
     1981 tcggccgcga actgcacaag ccgggcctcg tcgggggtat cgtcgttgac gaccgcgcca
     2041 tgaacgccgg cgcgcgccag gccgtgcgcg accttggcgc ggatatcgac ccggacgtgg
     2101 tggtggggcg gctttccatc gcgcagcgcc agcttatcca gatcgcgcgg gtgctgcttg
     2161 tgccgcaccg cgtggtgatt ttcgacgagc cgaccgcctc attgacgccg gtggagacgg
     2221 atgcgctgct gaaggtcatc accgacattc gcgccaaggg cgttggcgtg ttctacatct
     2281 cgcaccggct gcccgaggtg aaggagatcg ccgacaaggt gacggtgctg cgcgacggcc
     2341 ggctggtcgc cagccatccg gcatccgagc tggagccggt tgacatggcg cgcctgatgg
     2401 tcggccgcga cgtggccaag ctctatcccg accgacaggc ggaaacgggg cgcgaaaccg
     2461 tgctggaagt cgaggacttc tccgttccgg gctatgcgca aaacgcctcg ttcaagctgc
     2521 gcaagggcga gatactgggc tttgccgggc tggtcggcgc gggccgcacc gaattgatgg
     2581 agggtattgt cgggcttcgg cctggccgtg gcgcgctgcg catcggcggc aagccggccc
     2641 agttcgccaa tgtcgatgcc agcctcaagg cgggcatcgc ctatctcagc gaggaccgca
     2701 agggcaaggg cctgttgctg accgaggatt tgcgtatcaa tctgacgcta gcctcgctgg
     2761 acaggttcca gcgcgggatt cagatcgaca gcgccaggga gcgcgccgcg ctcgacaagg
     2821 cgatcgccga gttcgacatt cgcgccagcc gcaaggacat gctggccggc cagctttcgg
     2881 gcggcaacca gcagaagctg ctgctggcca agatgatgat gaccgatccc ggcatcatca
     2941 tcatcgacga gccgacgcgc ggcatcgata tcggcaccaa ggaacagatc tataaattca
     3001 tcgccgagct ttcggcgcag gggcgctcga tcatcgtcgt ttcctctgaa atgcaggaac
     3061 tgatcggcat ttgcgaccgc atcgtcgtca tgcgcggcgg caggctggtc ggcgaggtcg
     3121 ctggcgaaca tatgaacgaa aacgagatcg tcgtgctggc gacgggcgta aaatccgggg
     3181 aggccgcata gcggagccac aatgaccgac ctcgcagcga caaatcaaaa agtacgcaaa
     3241 tcctggaagg acatcgacct gcgggcggtc gcgcccttcg tagcactcgc ccttttgctg
     3301 gtgctgggcg catttgccaa tccgaacttc ctcggcatcg acaacctgct caacgtcatt
     3361 acgcgcagcg ccttcatcgc catcatcgcc attggcgcga ccttcgtgat cgcctccggc
     3421 ggcctcgacc tttcggtcgg ctcgatggtg gcgttcgtcg ccagcctgat gatcctgttc
     3481 atgaacagcg gcctgatcgc cgactcggcc ctgatgctgt ttgccgccat gggcgtggcc
     3541 gtcctcgttg gcctggcctg cgggcttgcc aacggcctcg tcaccacggt cgggcgcatc
     3601 gaacccttca tcgccacgct cggcaccatg ggcatctatc gcggcctgac cacctggctg
     3661 tcgcagggcg gcgccatcac ggtgaagtcg ccggaaatcc agacgctcta ccgcccggcc
     3721 tatttcggcg cggtgctggg ggttccggtg ccgatcatcg tcatcgcggc ggtcgccctc
     3781 atcggcgcgt tcatcctgca ccggacgcgc tatggccgcc atgtgctggc ggtcggctcg
     3841 aacgaggacg tggcgcgcta ttccggcatt ccggtcgacc gcgtgcgcac cgtcacctac
     3901 atcatccagg gcctgtgcgt ggcggtggcc tgcctgctct acgtgccgcg cctcggctcc
     3961 acctcggcca ccaccggcct gatgtgggag ttgcaggcga tcaccgccgt ggtggtcggc
     4021 ggcaccgcct tgcgcggcgg cgtcggccgc atctggggca cggtgtgcgg cgcgttcatc
     4081 ctggaaatcg tcggcaacat catgctgctt tcgaacttcg tcagcgaata tctgatcggc
     4141 gctatccagg gcgcgatcat catcatcgcc atgctcgtcc agcgttcgct ggtgcggaaa
     4201 taaaggatcg gccgggccaa cggggcaccc ggccttttat gcaacagacc ctgttgaact
     4261 atgggagaaa gaacatgcgc aaacgaatgc taggcttcgc tgccgtcgcg atggcagcct
     4321 tgatgagcgt ctcgtacgct caggacaaga aggtgaccat cggcgtttcc attccggccg
     4381 ccgaccatgg atggaccgcc ggcatcgtgt tccacgccga gcgcgtcgcc aagcttctga
     4441 tgcaggagca tccgggcctg aacgtcatcg tcaagacctc gccggacgcc gcttcgcagg
     4501 cgaacgcggt gcaggatctg gccgtgcagg gcatcgacgc cctggtcatc ctgccgtccg
     4561 atccagaccc gctggtcaac gccatcaagg aagtcaaggc caagggcgcc ttcgtgacgc
     4621 tggtcgaccg tgcgccgtcg agcaacgacg attccgtgcg tgacctctac atcgccggca
     4681 acaatccggc tctcggcgaa gtcgccggca agtacatcaa ggacaccacg cccgacgcgc
     4741 aggtcgtcat cattcgcggc ctgccgatcc cgatcgacca gcagcgccag gacggcttcg
     4801 acaagggcat tgccggctcc ggcgtcaagg ttctcgaccg ccagttcggc aactggaacc
     4861 gcgacgacgc cttcaaggtg atgcaggact acctgaccaa gtacccgaag atcgacgtgg
     4921 tgtggtgcca ggatgacgac atggccgtcg gcgtgttgca ggccatcgaa caggccaagc
     4981 gcaccgacat ccagtatgtc gtggccggcg ccggctcgaa ggacatggtc aagaaggtca
     5041 tggacggcga caagctcgtt cccgtcgacg tgctctatcc gccggcgatg atcggcaccg
     5101 cgatggaact gaccgccaac aacttctacg atcaggttcc ggtgcgtggc tcgttcatcc
     5161 tcgacgcaac gctcgtcacc aaggacaacg ccaaggactt ctatttcccc gattcgccgt
     5221 tctgatcgcg tttcggatag ggacgggtcg atgctcttct cggcccattc cagcaaaacc
     5281 cgccggtccc ggcgggtttt gtcgttcatg aaaggcggca gccaggttga acgccggaga
     5341 ggcaaggcgc aatcgcgctt gcccgcccgg ccggtgccga ccttcaaacg gcccggcatg
     5401 gattattctt cgctggcggg tgttctcctg tcagcgccgt cttttcgagc ggccgtggaa
     5461 ggatatttcc gatgcatagg acgttattcc tagtctgttc ttttctgtgt tccgccatgt
     5521 ttgccgccca tgccgacacc ttgtcggacc tgcaaggccg ctatgcgatc cagccatcct
     5581 cgcgggtggc gttttcggtg gcccaggtcg gcggcggcgg catcgacggc gttttccagc
     5641 atttctccgg cacgttcgac ctgcaccccg acaatctcgc ccgctccagc gtgaatttct
     5701 cgcttcaacc cggcagcgtc gccaccggcc agtcgcgcgt cgatgatttc ctgcgctctt
     5761 ccgccgtctt caatgtggcc gcctatccgg tcatcagctt ccgctcgacg gcggttcgcc
     5821 agcaaggctc cgaccacgcc gtcatcgaag gcatgctgac ggcgaggggc atcgcgcatc
     5881 ccgagacgtt tgatgcgacg ctggtcaagc ggcaggggcg cacggtcgcc ttccatgtcg
     5941 tcggccagat gctgcgctcg ccctatggca tggatgtcgg cacgccgatc tattcgaacg
     6001 tcgcccgctt cgacatggtg ctcgacgggc agcggaaata atcgtctcgc cgaagaaatc
     6061 ttccaccggc gggattaatc gccgccgggc ggtgttgttg cgatacgggc ggaaaatccg
     6121 ctcgtctcga atgcaaaaga agaggttttc agatgatgcg ttctctcata ttggcggctg
     6181 tcatgatgac ggcggcgatg ccccttgcct atgcggatga cgactatgcc ggcggcgcca
     6241 tcaagtcgat ggatacggcc aagggcgagg ttctcaccga cgccaagggc atgacgctct
     6301 atacgttcga caaggatgcg ggcggcgctt cgaactgcta cgatgcctgc gcgaccaagt
     6361 ggccgccact ggctgcggca gccggtgctg cggtagaggg caagtatacg ctggtcgacc
     6421 gcaaggacgg ttcgaagcag tgggcctatg acggccaccc gctttacctc tggcagaacg
     6481 acaagaagcc gggcgatgtc accggcgacg gcgtcggcgg cgtttggcac gccgccaagg
     6541 aataggttac gggcaatagc cccaggaaca gcctatgtcg caggtcatgg acgagatcga
     6601 ggaatgcgtg ccggcgctcc ggcgctatgc gcgcgcgctg acgcacgatc ccgatcgtgc
     6661 ggacgatctc gtccaggact ctcttgagcg cgccatccgc aagcgtggcc tgtggcagcc
     6721 ttcgggttcc gtgcgctcct gggtattccg gatattgctg aacgtgcacc gcaacgagtt
     6781 gagaaggctg cggcgcgcgt tcacgccggt ttccctcgaa gccctgccgg gcggcgaacc
     6841 ggcgggcctg gacgtgcagc cgggccggct ggcgctggcc gagacggcac gtgccatgca
     6901 ggcgctgccg gccgaacagc gcgaggtgct gcttctcgtc gccgtcgagg agatgagcta
     6961 tgccgaggcg gcggcggtgc tttcgatccc gcttggcacg ctgatgtcgc ggctggcccg
     7021 cgctcgtgcc gcgcttcgcc atatgacgga gacgggtgcg ccgaaattga gggccgtgaa
     7081 atgagcagcg aggaacacaa catcacaccg cacgaccttc acgcctatgc cgatggcttg
     7141 ctggatggac cggcgcgcgc cgccgtcgag gcctatctgg cgcaaaaccc ggccgccgcc
     7201 gacgaggtgg aaggctggaa acggcaaaac gagaccctgc gcgcgcttta cggccatgtc
     7261 gccgccgagc cggtgccggc gcatctcaac gtctaccgcg tcgagcgcga gagccggatc
     7321 aaggcgatgc gctggagccg gatggccgcg gccgccgtca tcctgcttgg cctcggcacg
     7381 gctgccggct ggtatgggcg cggttccttt gctgcgggtg tgcccgcaca ggtcgcgctg
     7441 gtcgacgagg cgatggaggc ccacagggtc tattcaggcg aagtcgcgca ccccgtggaa
     7501 gtctgggcgg gcgagaagga ccatttgcag acatggctgt cgaagcgcct cgcccgcacg
     7561 ctggccgtgc ccgacctcaa ggctgccggc cttgatctgg tcggcggccg gctgttgccg
     7621 gcggcgagcg gcccagcggc gcagttcatg tatgaagacg acgccggaca ccgggtgacg
     7681 ctctatatca tccccgcgca ggagggccgc gagacctctt tccgctacgc ctcgctcgac
     7741 cggcttgaag cgttcttctg gaccgacgag acgatcagct gcgcgcttgt cggtgacctg
     7801 ccgcgcgctc gcctccagga aatcgccacc caggcctaca agcaactggg gtgacgcgga
     7861 atgttgcgca acggcggtga cacatatggg aaggtgtcga tcgccttcca ctggaccatc
     7921 gcgctcctgt tcttcgggca gatcgcgctt ggctatctga cgcaggcgac ggcaagcgat
     7981 cctcggctgc aattcgacct gtatcaatgg cataagtcgt tcggcttcct cgtgctggcg
     8041 ctggctctgc tgcgggtgct ttgggccttg tcgggcgtct ggccacggcc gctgccgggc
     8101 gctgcgcgtg gcgaggcggc tgccgggcgg gccgcccacc tgctgcttct ggcgctgacg
     8161 ctggccgtgc cgcttaccgg ctgggcgatc gcctcgacct cgctgttgcg aattccaagc
     8221 ttcatgttcg atcttgtggt catccccgac ctgccgatgg cgcgttccga cgagatggag
     8281 gcgttctggt caggggcgca tgaatggctg gcttacggag ccggcctcct ggcgcttgtc
     8341 catgcaggcg cggccatcca tcatcacctt atccgcaggg acgcgacgtt gaggcggatg
     8401 ctcggcgcag ggccgaggcc gtgaaacacc gcgccatctc tttgttttgg ccgcatttcc
     8461 agacgcaaaa ccgtttcgca cttttgctgg aaatgctcta ggaggcgctc tttcgcccgt
     8521 ggtggccgct gacctgaacg ccgtcttcgg gccgcagcag gaaaaaaccc ggaatggaaa
     8581 gaagcgggat gatggcggcg aacaggaaca cggtgtggaa gctttccggc gtcaacgccg
     8641 ggttttccca cgcgaccagc cccagcagca tcgcgccgat ggaaacacca aagctgacgc
     8701 tgagctgttg caggacaccg ccgaggctgg ttgcgctgct gagcttttcg ggcggaagat
     8761 cggcgtagga caaggtattc gaggtcatga actgcgccgc gcggatcagc ccgaaaagcg
     8821 cgacataggc tccgatcagc caggcggacg tgtccggctg caacagcgca aatccggcga
     8881 cggcggcgga gccggcaaac gcgctgccgg taagcaccgc gccgaagccg aacaggcgca
     8941 gcatcgaggg cagcagcggg cggatcagca gcgcgcccag gctgctgacg aaggtcagcg
     9001 agccagaggc gacggggctc atgtggaagc cgacctgcaa catcagcggc aaaaggaaag
     9061 gcacgccgtt gaggccgacg cggcagagcc cgccggccag cgtgccgacc aggaaagacc
     9121 ggaaccgaaa cagcgtcaag tcgaccgccg gcgcggcaat ggtgcgcgca tagcgcgaga
     9181 aggccaccag cagcaggatc gcggcgagca ggatggcgat gacgaccggc agcgggagca
     9241 cggacctgcc gatgttttcc agcccgaatt gcagcagcgc gacaccggcg ccgaccatga
     9301 cgaagccgac gaaatcgaag cggcgcgggg cctcgccgcg aacgtcctcg acaaagcgca
     9361 gcgccagcag gatgccgaga cagccgaagg gaacattgac gtagaagatc cagcgccagg
     9421 aagcgtaggt cgtcagcacg ccgccgagca gcggtcccat gaccggcccc atgacggcgg
     9481 gaagcgtcat gtacagcatg gcggtgacaa gctggctgcg cgggaagctg cgcagcagga
     9541 tcagccggcc gacgggcgtc atcatcgcgc cgccgaggcc ttgcagcgcg cgcgtcgcca
     9601 gaagcatctc gaaactgttg gccagcccgc acaggacgga gccgagcgtg aaggtaaaca
     9661 gcgccagcgc gaaaatccgc cgcgcgccga acctgtcggc gaaccagccg ctgaccggga
     9721 tgaatacggc gagcgtcagc agataggtgg tgatcgccag gttgagccgg atgggcgtgg
     9781 tggcgaggct tttcgccatt tcgggaatgg cggtggcgat gatcgtggaa tcgaggtttt
     9841 ccatgaaaaa cgcgacggcg acgatcgccg ggatgatcag ccgcaggcgc gggtcgcgtt
     9901 cggtcgcaaa ctgggccggg gcagatggca tggtagagtc cgtcatgggg cgttcctaag
     9961 tccggcccgg tgaatcggca acacccggcc cgctttcgac gttcacgaat gcgttggcgg
    10021 gcaatggctt ggcgagccta ccgagtcgtc aagctggcca gcgcttcgat cagctaaccg
    10081 gattttctgc tccggactta tggctgggcg aagcgcaatg ttcccgctag acgatgacgt
    10141 agaccagtcc ctcgtcgctg acgacatcga aggttgtcgc caccagcctt tcgccggcct
    10201 cacagggcga tgcgtcgccc acggcggttt cgtagctgcg caccctgacg cgcttcgggt
    10261 cgatctgcga caggcccgtt tcgatatcga attcccaatg gtgccacggg cagcggatga
    10321 ccatgttgcg ccggcaatag aggtgctggc cgggagcggt cgcccgtagc tcgccgatct
    10381 ggtcgccctg cgacagcggc ccgccctgat gggggcattt gtcggccagc gcgtagaaag
    10441 cgccgtcgac gtggaaaacc acgatggcgc ggcccgccac ctcgaccttc aatcgcccgc
    10501 catccggaat gtcgccgatg gcggcgaccg cgtggcgcgt catcgcgccg tgccgtagag
    10561 cgcggcggca ttgtcgtgga ggatggcgcg cttcagtttc gcatcgatgt gcggcggcag
    10621 cgaatggcgc ggatcgtcga aatcccagtg cggatagtcg gacgagaata ccacccggtc
    10681 atggccgatc cagtcgagca catcgacgag gtggcgcttg ttttccggct cctccatcgg
    10741 ctgggtggcg acccacagat gctgtttcag atattcggaa ggcggccgct tgacgtgcgg
    10801 aacctcgctg cgcatgcgcg cccatatgcg gtcgagccgc cagccgagcg ccggtatcca
    10861 ggcaaggccg gcctccagca aaacgatgcg gatgcggggg aagcgttcga acaggccctc
    10921 gaagatcatg ctggccagca ccgcctgggt cgagggcgcg tgctctgtca tttcctccat
    10981 gtagtaggag ggccagccgg cgggcgtgat cgcctggccg gaatagccga agacatggat
    11041 gccgaccggc aggtcggccg cctgcgccgc ctcgaagatc agccagtagc ggcgctggcc
    11101 gagcagttcg gcggtgcggc ttagcagaag cacctgcgcg aagcggcggt cgcccgcgaa
    11161 atgctcgatc tcggcgcggg caagctcggc gttctcgtaa ggcacgcaga tcgagcactt
    11221 cagccggggt tccggcgcgt tgtagaggtc gagctgccac tggttggtgg cgcggcacaa
    11281 ggctgccgaa tagtcgtcgc ccagcacgcc ctggccggtc ggccaaagcg ggttcatgat
    11341 gccgaattcc atgtcgtaga ggtcgagcag ctggctttgc agcaacccga catccgagcc
    11401 gggcaggccg ccatcgtccg gccagctgtc gcgccgcgac gaggcgggcg cggctttcgg
    11461 gtaggggttg ccgtcctgat agcccatgcg gtagcgcagg ccataggttg cgagatgcgc
    11521 cttccagcgt tcgcttaaga acggcatgag gtcgttcacc gaagtcagct tggggtgaac
    11581 gtcgcagtcg atgatctttt gcgtgacggg cgtttgcgaa gtcgcggccg gctggtgcag
    11641 gttcatgggc ggacctccga caggcgaggg taggtggcgg cggggttttc gaccgcgatc
    11701 ttgcgcacca tggcggcatc gaagccggcc ggcagcatgg cgtcgccatc gaactgccag
    11761 tgcggataat ccgacgaaaa caacagcatc tcgtccgatc cgatgtggtc gatgagacgc
    11821 gacacgatgt gcggatcgtc gggaacgtcg aaaggctggg tggtcaggcg cacatggtcg
    11881 cggatgattt ccgccggcgg ccgctcgacc cagggcgtct cgaagcgcag gcccttccag
    11941 aacttgccga agcgccacag gaaagcgggc agccagctga tgccggattc cagcagcacg
    12001 accttcagcg aggggaactg cgtgaagacg ccttccgaga caaaactggc aagctgcgtc
    12061 tgcatgatct gcgattgggc cgcgtaatcc tccgccaccg tgttcggcca cccaagcgag
    12121 gtgacagggt ggtggtaggc gctgccggca tggatgccga tgggcaggcc gtgtcgcgcg
    12181 gcggcctcgt acagcggcca gtagctgcga tggccatagg gcatgtgggc ggcggccggc
    12241 agcaggattt gcacgaagcg gcggtcggcg gcgcagcgct ccacttcggc aatcgccgct
    12301 tcgatatcct gtaacggcgc cacgatggag gcgcgcaggc ccggatgcct gtccagccac
    12361 tcgaaccgcg tccagtcgtt cagcgccctg gtcacggcga tggccatgta gctgtcgtag
    12421 agcatgtgga cgccgcagag attgttcagg atgcccgtct ccgcgccggg cctgtcgagc
    12481 gcctctctcg ccagccgctc gggcgtgttc gacaggccgc cgctttcatc gcgccagtcg
    12541 gggcgacagg tcagcggcgc attggcgggg taggcgatgc tgtcgagctg gccaatgccg
    12601 cgcgccgcga tcaggtcctg ccatacgtcg gccagatagg gcatcagctg gctcatcgtc
    12661 ggattggggt ggtggatgtc gcaattcacg ctgcccttcg gcagcaacgc ggttgcggtt
    12721 tccatcgaca atgccatttg cctgaacctt cggtataagc gccttgcgaa acctttgccg
    12781 gcaagggtga cggattgccg cccatggctg catcattttc ccgccaatat tgacgaattt
    12841 tccgcctgtt ttcacttgca cgcggcgcgt ctaggtcgtg ggccggcgct cgtgcgaggg
    12901 caggcaggcg aagcgcgcgc gataactctg cgcgaaataa gactgcgagg aaaagccggt
    12961 atcgagcgcg acgcgcagga tcggcagcgg cgtttgccgc agcagcgcgc gcgcccgctc
    13021 cagccgcagg ccgatataat agcggccggg cgtcgtcttc atttcggcgg cgaacagccg
    13081 ctccatttgc cgcgccgaca ggcctgcggc cttggccagt ttttcgatgg aaagcggatt
    13141 ttccagattg tcggccatca gttcgatgac ctggcgcagc accggggatt tggtggccag
    13201 gtcgcgcccc ggaccgacgc gctggcggtc gctggtctgg cggacacgct catgctggaa
    13261 ctgcccggcc acctcgtcgg cgatgggcgc gcccagatcg cgccggatga tttcaaggaa
    13321 caaatccatc gaggtcgtgc cgcccgccga agtgtagcgc ttggcgtcga tctcgtagac
    13381 attggcgctg cattcgatgt cggggaagct ttcgcgaaaa ccggcgcggt tctcccagtg
    13441 gatggtgcag cggtggccgt cgagctgccc agactgcgcc agtatatggc tgcccagcgt
    13501 cagcgcaccg agcgccgcgc cggctctgcc ccatcggcgc aaggcggaca ggaccgcggc
    13561 tttctcgggc acgtcgatct gcgttccggc gcagacgaag acaatatcgc aggcaggcac
    13621 gtccttcagc gaatgatcga cgttgagctt catgccgttg gaggcgatca ccggctggcc
    13681 gtcgaaagag atcgtcaccc agcgatagaa ttgccgccca agtgtgtggt tggccgtacg
    13741 catcgtatcg acggcggcgg caaggctgaa catcgggaag cgatgaacga gcagaaatcc
    13801 gatgacgcgc atttccgaag tggcctctgc ggcatttaca ttcgtcaatt ttttctcggc
    13861 cgcttgaaga caatgcaggc ttggacagat ggttcggcat cgaacgtatc attcgggccg
    13921 gccggcgcaa atgtcccgac ggcctgcgcc atgtatttgg caagaggctt gccccgccgc
    13981 ccgtcctaca gctttgtatg atgaaatcgg aaggcgcgct cggcggcctg cgcgagccgc
    14041 cggggtagac ggcgttgatt ttcgcgcgct ctgccttcac cgcttcagtc agcgcttcca
    14101 tccgctcagc atcaagaccg tcgcgcgtga cagtcgcccg gccgtaccaa cccgaaatcg
    14161 gccaccgcca tgcgcgccgt ttcatcacct gcaagcagca tgccggtcga cgcggccgct
    14221 tccctcagcg gtcataccgg cgcggaaagg cgaaagtcga agccgggccg tttgcggcgg
    14281 cccctttttg ggaccgccgt tgtttgagtt ttttacaccg ccagatcgtc gggcagcagc
    14341 gcgtcgggca ggttctggta agcgaccggg cgcaggaagc ggcggatggc cagcgtgccg
    14401 accgaggttg tcgcggtgtt cgtgctggcg gggtaggggc cgccatgcat catggcgtcc
    14461 accacttcga cgccggttgg aaaaccgttg gccaacagcc ggccggcctt ctgttcgagg
    14521 atcggcacca gcccgcgcgc ctgctcggta tcgccggcgt cgatctgcaa ggtgacggtc
    14581 agttggccat gcaggccctc ggcgatggcc gccatctggc cggcgtcgct ggcgcgcacg
    14641 acgacgccga gcgggccgaa gacctcctcg ccaagttcgg gattggccat gaaggtctcg
    14701 ccgtcggtga cgaacagggc aggggcggcg ttgcggtcct gcgctgcgcc ttcataggcc
    14761 gaacgcaccg cgttgctggc ggcgatgcgc gccgcgccgc gccggaacgc ctcggcgatg
    14821 cccgtcgtca gcatcggctg cgcggcgatg ccggccagcg ccgtgcgcgc ttcctgctcg
    14881 aaaatatcgg cgggttcgcc gtcctcgatc acgacgatgc ccggattggt gcagaactgg
    14941 ccgacgccca tcgtcagcga tccggcccag ccctttgcga tttcggcggc gcgggcgcgc
    15001 gcggcctgcg gcaggacgaa catcgggttg accgagccaa gctcgccgaa gaacgggatc
    15061 ggctcttcgc gccgcgccgc caggtcgaac agcgcgcgcc cgccgcgcag cgaaccggtg
    15121 aagccgaccg ccttgatcag cggatgggtg accagcgcct cgccggcctc gttggtgttc
    15181 gactgcacca gcgcgaacac gccggggtgc tggccggtct tctttatggc ttcgaggatc
    15241 gcctcggcga cgatctcgcc ggtgccgggg tggcccggat ggcccttgac gacgaccggg
    15301 cagccggcgg ccaacgcggc ggcggtgtcg ccgccggcgg tggagaaggc gagcgggaaa
    15361 ttcgacgcac cgaacacgcc gaccgggccg atgggccgct gcaccagctt gataccgggg
    15421 cgcgccgccg gcttgcgctc gggcagcgct gcgtcatggc ggcggtcgag gtaagcgccg
    15481 tcgcggatgt ggctggcgaa cagccgcagt tggccggtgg tgcggccgcg ctcgccttcc
    15541 agccgcgctt caggcaggcc ggtttcggcc gagccgatgg cggtgatggc cgcgccgcgc
    15601 gcatcgatcg cctcggcaat cgcttcgagg aaggcggcgc ggctttcgcg cgaggtccag
    15661 ccatagacct tgaacgcttc ggcggcggcc tttgcggcgc ggtcgatttc agcggtggcg
    15721 ccgagcgaga aacgatgcgc ctcgcccttg gccggatcgg aggcgaaggt cgcttcactt
    15781 gccacccatt ggccggcgat aagatgtttt ccgtgcagcg tatgggcctg cgtcatgatt
    15841 gtggtcctaa tcctcgaatg cttcgacggt ttgccgctcc cccagcatga aagcgtcggc
    15901 gacatggaca agcggcgcga ggtcgacatc gacctcgcct gatcgcacca gttcggcaaa
    15961 gcggcggtag atgccggcat attcgcgatc gtcctcgacc acaaccggct tttcgtcgat
    16021 tgtcatttca gcgccgcctt tcgacagaac aagccggccc tcggcggtgg cgacctcgat
    16081 atcccaggtc tgcggtccgg tctggcgcca gtcgaaatcg gcgctggccg tcacgccgtc
    16141 ctcgccttcg aaggccagtt gcgcggcgat cggggctgcg cgccctgccg ggaattcgag
    16201 gttcgaacgc accagatgca tcggcctcgg caagatcgcg gtgaagatgg aaagcgcatt
    16261 gatgccgggg tcgaagacgc ccagcccgcc cggctgccag atccaggcct ggccggggtg
    16321 ccagcgccgc acatcttcct tccaggtcac ggtaacgtgc cggatggtct tgtcggccag
    16381 ccagcgccgc gccggctcga cggccgcggc aaagcgcgaa tgccaggtgg caaacagcgc
    16441 aagacgcctt tcgctcgcca tttccaccag cgcctgaacc tcgctgagcg tcgcgcccgg
    16501 cggcttttcc aggagcacat ggcggccggc gcgcagcgcc ttggcggctg cctcaaagcg
    16561 cacctgcggc ggctggcaaa gcgccaccgc ctgaatgtcg gggcggttgc cgagcagcgc
    16621 ctcgatgctg tcgaacgcct cgacgccgtc aatggcgccg ctgcggctgg cggtggccgc
    16681 aagctggaat gccggattgc cgaggatcga cggcacatgc tggtcgcgtg cgatcttgcc
    16741 gacgccgacg attgccaatg agattggggc cggggagata ggggccggcg aagtgggctt
    16801 agtggctgtc acggggcacc gcctttccgc gtccgccctt gaggaagtcg aaatccgcgc
    16861 cggtgtcggc gccctgcaca tgggttgcga acagccgggc ataaccgctt tcgggcaccg
    16921 ccatcggcgc cgtccacgcc gccagcctgg cggcgatttc cgcttccggc acatcgagat
    16981 gcagccggcg tcccgccacg tccacctcga tcatgtcgcc attgcgcacg atggccagcg
    17041 gcccgccgac cgcagcttcg ggcgaaacat gcagcaaaac ggtgccgtag gctgtgccgg
    17101 acatacgggc gtcggaaatg cgcaccatgt cggtgatgcc cttgcgcagc accttcggcg
    17161 gcaggcccat attgccgacc tcggccatgc cgggatagcc gcgcgggccg caattcttca
    17221 tcaccatgat g
//
LOCUS       DASYCS010000248         4029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3975166,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000248 DASYCS010000000
VERSION     DASYCS010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3975166"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1288
                     /locus_tag="VGM46_08050"
     CDS             <1..1288
                     /locus_tag="VGM46_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4192586.1"
                     /translation="FLIDSGTALNSPSWQASVGDIVPRSKVPAAIALNSLGFNLTRSV
                     GPAIGGIIVAAAGAAAAFAANALSYIALIVVLFRWKPQLAENTLPRESLGAAMGAGLR
                     YVVMSPNIGTVLARASAFGFTAGSVLALLPLIARDLIHGDALTYGILLGAFGVGAVCG
                     ALTSVRLRQMLSSETMVRWAFAGFALCAFNAAFSHHTWQTAMGLFIGGACWVIALSHF
                     NVTVQMSTPRWVVGRALSIYQTANFGGIALGSWVWGVVADAHGVDTALIAASLTMLAG
                     GALGLLLPLPEHAALNLDPLNRWTEPMLALDLKPRSGPIAIMIEYTIEEKDLPEFIAL
                     MAERGRIRRRDGARNWTLGRDLADPQLWTESYHTPTWLDYIRHNKRATHADAAITERI
                     RALHSGSELPRVRRMIERPTSADHGLGAPAGRSDRH"
     gene            complement(1310..3040)
                     /locus_tag="VGM46_08055"
     CDS             complement(1310..3040)
                     /locus_tag="VGM46_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006338868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEY4192587.1"
                     /translation="MRSIPVLQARGIAVVGLAVIVGVVAGVMVTAMSQMVQSLHWLLF
                     GLQPGARLSAMFALQSPLQALMPAIGGAILGLTIICLRKWKFRTPIDPIEANALYGGR
                     MSLTDTFIIAVQTMISSGFGASVGLEAGYTQVGSGLASRLARLFRLRRNDARILVGCG
                     AAGAIAAAFDAPLTGAFYGFELVIGIYSVGNVAPVMAAAISASLTAEIFGGAPFPLEL
                     SGLPALTASQYVPFIILGLLGGGVSIAIMYLVTLTERLFVKMRINGSLRPFFGGCAVG
                     LLGLITPQVLSSGHGALHREFAMNYGLAVVTSVFLLKLAASAISLGSGFRGGLFFASL
                     FLGALLGKMFAGVMAFALPGIGIDPSVAAVVGMTSLAVGVVGGPLTMTFLALESTRDL
                     TLTGVVLAASIMAAILVRETFGYSFSTWRFHLRGETIRSANDIGWMRSLTVEKMMRKD
                     VKTIDGSTTLSAFRKAVPLGSAQRVIAIDDEGRYAGVLIVADAHSDLSEGDSSVLSLA
                     KFRDAVLVPTMNVQSAAETFQRAGSEELAVVKDFTDRVVVGLLTEGHLMRRYAEELDK
                     ARRDLSGEGS"
     gene            3039..3245
                     /locus_tag="VGM46_08060"
     CDS             3039..3245
                     /locus_tag="VGM46_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192588.1"
                     /translation="MSTTRRRRARVMEEFTEFTYGSGEGFAALIRLAYVHGKAAFPPS
                     RKLERLRLDGPGIRLAPGDDFCPE"
     gene            3248..>4029
                     /locus_tag="VGM46_08065"
     CDS             3248..>4029
                     /locus_tag="VGM46_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HEY4192589.1"
                     /translation="MAISESISAGALPKNSRDIFFALGIVFILAVLFLPIPAFLIDVG
                     LAFSIALSVLILMVALWIQRPLDFSSFPTILLIATMLRLSLNIATTRMILSHGNEGTH
                     AAGYVISGFSKLVMSSDFVIGLIVFMILIVVNFIVITKGATRIAEVGARFTLDAIPGK
                     QMSIDADLSAGMIDDKTAQLRRRELEEESSFFGSMDGASKFVRGDAIAGLIITAINIV
                     GGIAIGYIRHGMGIGEAADVFIKLSVGDGLVTQIPALIVSLAA"
BASE COUNT          725 a         1376 c         1220 g          708 t
ORIGIN      
        1 cttcctgatc gattcgggca ccgcgctcaa cagtccctcc tggcaggcct cggtcggcga
       61 catcgttccc cgctcgaaag ttccggcggc catcgcgctc aattcgcttg gcttcaacct
      121 gacgcgcagc gtcggcccgg caataggcgg catcatcgtc gccgccgctg gtgccgccgc
      181 cgccttcgcg gccaacgccc tgagctacat cgccttgatc gtcgttctgt ttcgctggaa
      241 gccgcaactg gcggaaaaca cgctgccgcg cgaatcgctg ggcgccgcca tgggtgccgg
      301 cctgcgctat gtcgtcatgt cgccgaatat cggcacggtg ctggcgcggg cgtcggcctt
      361 cggcttcacc gccggttcgg tgctggccct tttgccgctc atcgcgcgcg accttatcca
      421 tggcgatgcg ctgacttatg gcatcctgct cggcgcgttc ggcgtcggcg cggtctgcgg
      481 cgcgctcacc agcgtgcggt tgcgccagat gctgtccagc gagacgatgg tgcgctgggc
      541 cttcgccggc tttgcgcttt gcgccttcaa tgccgctttc agccatcata cctggcaaac
      601 ggcgatgggc cttttcatcg gcggcgcgtg ctgggtgatc gcgctttcgc acttcaacgt
      661 caccgtgcag atgtcgacgc cgcgttgggt ggtgggccgg gcgctgtcga tctaccagac
      721 cgcgaatttc ggcggcatcg cgcttggcag ctgggtatgg ggcgtggtgg cggatgcgca
      781 tggcgtggat accgcgctta tcgcggcgag cctcactatg ctggccggcg gcgcgctcgg
      841 cctgctgctg ccgctgccgg aacatgccgc gctcaatctc gatccgctca accgctggac
      901 ggagccgatg ctggccctcg acctcaagcc gcgcagcggc ccgattgcga tcatgatcga
      961 atatacgatc gaggagaagg acctgccgga attcatcgcg ctgatggccg agcgcgggcg
     1021 cattcgccgc cgcgacggcg cccgcaactg gacgctgggg cgcgacctcg ccgacccgca
     1081 attgtggacg gaaagctacc acacgccgac ctggctcgat tacatccgcc acaacaagcg
     1141 cgccacccat gccgacgcgg cgatcaccga gcgcatccgc gcgctgcaca gcggcagcga
     1201 actgccccgc gttcgccgca tgatcgaacg cccgaccagc gccgatcatg gcctgggcgc
     1261 accggccggt cgcagcgacc ggcactgagc gccggagagt gtcctgacgt cagctgccct
     1321 cgcctgaaag atcgcggcgc gccttgtcca gttcctcggc atagcggcgc atcaggtggc
     1381 cctcggtaag aagcccgacg accacacggt cggtaaagtc cttgaccacc gccagttctt
     1441 cgctgccggc gcgctggaaa gtttcggcgg ccgattgaac gttcatggtg ggcaccaaaa
     1501 ccgcatcccg gaacttcgcc agcgaaagca cggagctatc tccctccgaa aggtcgctgt
     1561 gcgcatcggc gacgatgagc acgcccgcat agcggccctc gtcgtcgatg gcgatgacgc
     1621 gctgcgccga gcccagcggc accgccttgc ggaaagccga aagcgttgtc gagccgtcga
     1681 tggtcttgac gtccttgcgc atcatttttt cgacggtcag gctgcgcatc cagccgatat
     1741 cgttggcgct gcggatggtt tccccgcgaa gatggaaccg ccatgtcgag aaggagtagc
     1801 cgaacgtttc ccgaacgagt atggccgcca tgatcgaggc cgccaggacc acgccggtca
     1861 gcgtcaagtc gcgggtcgat tccagcgcca ggaacgtcat cgtcaacgga ccgccgacga
     1921 cgccgaccgc cagcgaagtc atgccgacga ccgccgcgac cgacggatcg atgcctatgc
     1981 ccggcaaggc gaaggccatg acgccggcaa acatcttgcc cagcagcgcg cctaggaaca
     2041 gtgaggcgaa gaacaagccg ccgcgaaagc ccgaccccag cgagatcgcc gacgccgcca
     2101 gcttcaacag gaagacgctg gtgaccacgg caagcccgta gttcatcgcg aattcgcgat
     2161 gcagcgcgcc atggccgctg gacagcacct gcggcgttat aagccccagc aggccgaccg
     2221 cgcagccgcc gaaaaacggc cgcagcgaac cgtttatcct catcttgacg aacagccgct
     2281 ccgtcagcgt caccagatac atgatcgcta tcgatacgcc gccgcccagc aatcccagaa
     2341 tgatgaacgg cacgtactgg ctggcggtca gcgccggcaa gccagacagt tcgagcggaa
     2401 acggcgcgcc gccaaaaatc tcggcggtca gcgatgcgct gatggcagcc gccatcaccg
     2461 gcgcgacatt gccgaccgaa tagatgccga tcaccagttc gaacccgtaa aaggccccgg
     2521 tcagcggtgc gtcgaaggca gccgctatgg ccccggccgc gccgcagccg accagaatac
     2581 gcgcatcgtt acggcgtagc cgaaacagcc gcgccagacg cgatgcgagg ccggacccaa
     2641 cctgcgtata gcccgcctcc agcccgaccg acgcgccgaa gccgctggaa atcatcgtct
     2701 ggacggcgat gatgaacgta tcggtcagcg acatgcgccc gccataaagc gcattggcct
     2761 caatgggatc gatgggcgtg cgaaacttcc atttccgcag acagatgatt gtcagcccga
     2821 gtatcgcgcc gccgattgcc ggcatcagcg cctgcaacgg gctttgcagc gcaaacatcg
     2881 ccgacagccg cgcgccgggt tgcagtccaa acaacagcca atgcaggctc tgcaccatct
     2941 ggctcatcgc cgtcaccatg acgccggcga ccacgccgac aatcaccgcc aggccgacaa
     3001 ccgcgatgcc ccgcgcctga agcacgggga tggatcgcat gagtacgacg cgaaggcgac
     3061 gggcgcgcgt gatggaagaa tttacggagt tcacatacgg ctccggcgaa ggctttgcag
     3121 cgctgatacg cctagcctat gtccatggca aggcggcctt cccaccctcg cgcaagcttg
     3181 aacggctaag gcttgatggt ccgggcatcc gtcttgcgcc cggcgacgat ttttgcccgg
     3241 agtaatgatg gcgatcagcg agagcatttc ggccggcgcg ctgcccaaga acagccgcga
     3301 cattttcttc gcgctcggca tcgtcttcat cctggccgtg ctgttcctgc cgatcccggc
     3361 cttcctcatc gatgtgggct tggccttttc gatcgcgctg tccgtgctga tcctgatggt
     3421 ggcgctgtgg attcagcgcc cgctcgactt ctcgtcgttt cccaccatcc tgcttatcgc
     3481 caccatgctg cggctgtcgc tcaacatcgc caccacgcgc atgatcctgt cgcacggcaa
     3541 tgaaggcacc catgctgccg gctatgtcat ttccggcttt tccaagctgg tcatgtcgag
     3601 cgattttgtc atcggcctga tcgtcttcat gatcctgatt gtggtgaatt tcatcgtcat
     3661 caccaagggc gccacgcgta tcgccgaagt cggcgcgcgt ttcaccctcg acgccattcc
     3721 cggcaagcag atgtcgatcg acgccgacct ttccgctggc atgatcgacg acaagaccgc
     3781 ccagcttcgc cgccgcgaac tggaagaaga aagctcgttc ttcggttcga tggacggcgc
     3841 ttccaaattc gtgcgcggcg acgccatcgc cggcctcatc atcacggcca tcaacatcgt
     3901 cggcggcatc gccatcggct atatccgcca tggcatgggc atcggcgagg cagccgatgt
     3961 gttcatcaag ctgtcggtcg gcgacggcct tgtcacccag ataccggcgc tcatcgtttc
     4021 gcttgccgc
//
LOCUS       DASYCS010000249         5775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_785529,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000249 DASYCS010000000
VERSION     DASYCS010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 5775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5775
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785529"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..426)
                     /locus_tag="VGM46_08070"
     CDS             complement(139..426)
                     /locus_tag="VGM46_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192590.1"
                     /translation="MRKIKAADKHAAINIERERRIAAGTTVTVAGVGDIPVQGHEVDV
                     YNLQGLGLGALAAVPLGDVATITIFRDAENVDHNLTPSQVLELVQKAAGVA"
     gene            complement(815..1678)
                     /gene="tatC"
                     /locus_tag="VGM46_08075"
     CDS             complement(815..1678)
                     /gene="tatC"
                     /locus_tag="VGM46_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEY4192591.1"
                     /translation="MEHLIELRRRLIWSIGGFFAAFLVCFFFAKKLFNLLVVPFKWAT
                     QWAGLDPHKVELIYTAPQEFFFTQIKLAMFGGMVIAFPLIATQIYKFIAPGLYKNERN
                     AFLPFLIASPILFLMGASLVYFFFTPMVMWFFLAMQQAGTDDQVQISLLPKVSEYLSL
                     IMTLIFSFGLVFQLPVVTSLMVRVGMLSSKALAEKRKWAIVIAFIVAAVLTPPDPMSQ
                     IGLALPTIILYEVAIWTARMIERTREKDRVAKEARDAAEEATEKEAERAAEKAAADKA
                     AAEKAAETPSA"
     gene            complement(1723..2601)
                     /gene="tatB"
                     /locus_tag="VGM46_08080"
     CDS             complement(1723..2601)
                     /gene="tatB"
                     /locus_tag="VGM46_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015317534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HEY4192592.1"
                     /translation="MFEVGWTEMLVIAVVMIVVVGPKDLPNMLRTFGRTVAKLRAMAG
                     DFQKQFNDALKEAELDDVKKSVDALRGLNPAAEIRKQLNPFEKAAADVRSGLDNVMKP
                     KPAADPSEPAAAEASAAEPLKNAGADMLGIPESDAPASTPAYPAMTDASVVTGSVPAA
                     EPAKVPAAKKATIAAPKAAKPAPAKPAPAKPASAKTEAAKPAARSAPAKSAPVKAASA
                     KPAPVPLAARSTPVANKAPPAKPVAAKPAPAKAVPAKAAAKQAPAKPAAIAKKSASTP
                     AKKPVTANKKTTGTAK"
     gene            complement(2637..2858)
                     /locus_tag="VGM46_08085"
     CDS             complement(2637..2858)
                     /locus_tag="VGM46_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019858561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEY4192593.1"
                     /translation="MGSFSIWHWMIVLVIVLLVFGRGKIPELMGDMAKGIKSFKKGMA
                     DDDVADDKRTVEHRADETVAAAKEKASKS"
     gene            complement(2993..3730)
                     /gene="scpB"
                     /locus_tag="VGM46_08090"
     CDS             complement(2993..3730)
                     /gene="scpB"
                     /locus_tag="VGM46_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015317532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEY4192594.1"
                     /translation="MSESTGASVLPFKADDEAAADGKSTMQRLHLAEAVRMAEAIVFA
                     SAEPVSARQLAARLPDGIDIDAVMAQLQQVYTRRGVNLVRVGDAWAFRTAGDLAFLMS
                     RDTVQQRKLSRAALEVLAIIAYHQPVTRAEIEEVRGVETSKGTLDTLMETDWVRMRGR
                     RRVPGRPVTYGTSDAFLDHFGLEEIRDLPGMEELKGAGLLSGRMPSNFSIPLPPSDPD
                     ALDEDEDPLTDIDLEELGLLTPRVTED"
     gene            complement(3727..4524)
                     /locus_tag="VGM46_08095"
     CDS             complement(3727..4524)
                     /locus_tag="VGM46_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009451717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HEY4192595.1"
                     /translation="MEAIWAEGEDSRLTGDPSLVVDVAGFEGPLDLLLHLARNQKVDL
                     SRISILALAEQYLVFIDQARAVRLELAADYLVMAAWLAFLKSKLLIPKQPGDEGESGE
                     ELAAVLQFRLKRLEAMRDAAARLVNRNRLGRDVFARGMPEMVIVEKRNAFTASLYDLL
                     TAYAIQRQKQAITNVRIARRGVWSLKQARDMLTRLVGSLADWTDLDQYLIEYLATPQE
                     RRTATASSFAASLELVREGTLEVRQQEAFAPIYLRGRQRPASFEGPS"
     gene            complement(4585..5607)
                     /gene="nagZ"
                     /locus_tag="VGM46_08100"
     CDS             complement(4585..5607)
                     /gene="nagZ"
                     /locus_tag="VGM46_08100"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEY4192596.1"
                     /translation="MTESKSMILGCAGKSLTREEIRFYRGECPWGFILFARNISEPEQ
                     IRDLVASMRDCIGRPDAPVFVDQEGGRVQRLRPPLAPNYPAGAALGALWRDDHDAGVR
                     AAWLLGRLHAFDLKSYDLTADCLPVLDVPIEGASDVIGTRAYGKEPISVTELGRAAAE
                     GLMAGGVLPVMKHIPGHGRAFSDTHFELPTVDAKLDELRRHDFEPFRQLAHLPAAMTA
                     HVIFSAVDPNNPATTSGKVIEEIIRREIGFDGLLISDDTSMKALSGDFPTKAASILAA
                     GCDLVLHCNGVFEEMAGIASRTTGLEGTALERARKALAVIGKADAVKEADIREEFATY
                     FEAVVA"
BASE COUNT         1011 a         1810 c         1824 g         1130 t
ORIGIN      
        1 cggcgagcgc tccgctacca aataaaaaga gcggctgtcc ggcacgacac ggctggccta
       61 cgccggccaa agatcgtcag ccgtcacacg gccggcagcg gctccatggc cttgatcgcc
      121 catgatggcg atgatcaact aagcaacccc ggccgccttc tgaaccagtt ccaggacttg
      181 cgacggcgtc agattgtgat cgacgttctc ggcgtcccgg aaaatcgtga tggttgccac
      241 atcgcccagt ggcaccgcag ccagcgcgcc aaggcctagg ccctgaaggt tgtacacgtc
      301 tacctcgtgg ccttggaccg gtatgtcgcc gacgcctgcg accgtcaccg tcgtgccggc
      361 agcgatgcgg cgctcgcgct cgatgttgat ggccgcatgc ttgtcggccg ccttgatctt
      421 gcgcaccaaa ctcccgctgc ttgttcgatg ccaaatgaaa ttgcgcgaac gatggctggc
      481 gttctggtct gttcaacacc cattgaaagc ctcgttttcc gggcttctct cgcctgttcc
      541 caccaaatcc cggctattcc cggttaaacc cttctagtac caactgatct tgcgtctcac
      601 acttctgttc tcacgttgcc ttgactcggc gcgagcagca acccctctcc catcatcccg
      661 gaaaccgcgt ggagcgcagc ggcacgcggc tgtccgggat ccatgccgtg acgataacga
      721 tccatgcaga gttcaatccg gaggcattac ggcatggatc ccggctctcg cctcggcatc
      781 ctgcgggtgc tcggcagcgg ccgggatgac gggcctaggc ggaaggtgtc tcggccgctt
      841 tttcggctgc ggccttgtcg gccgcggcct tttcagcagc ccgttcggct tccttctccg
      901 ttgcctcttc tgcggcgtcc ctggcttcct tggccacgcg gtccttttcg cgtgtccgct
      961 cgatcatgcg ggcggtccag atggcgactt cgtagaggat gatggtgggc agcgccaggc
     1021 cgatctggct catcgggtcg ggcggggtca aaacggcggc gacgatgaag gcgatgacga
     1081 tggcccattt gcgcttttca gccagcgcct tggacgacag catgccgacg cgcaccatca
     1141 ggctggtaac caccggcaac tggaacacca gaccgaagga gaagatcagc gtcatgatga
     1201 ggctgagata ttccgagact ttcggcagga gcgaaatctg cacctggtcg tcggtgccgg
     1261 cctgctgcat ggcaaggaag aaccacatca ccatcggggt gaagaagaaa tagaccagcg
     1321 acgcgcccat caggaacagg atgggcgagg cgatcaggaa cggcaggaag gcgttgcgct
     1381 cgttcttgta gaggccgggc gcgatgaact tgtagatctg cgtggcaatc agcggaaagg
     1441 cgatgaccat gccgccgaac atggcgagct tgatctgcgt gaagaagaat tcctgcggcg
     1501 cggtgtagat gagctcgacc ttatgcgggt cgagaccggc ccattgcgtc gcccatttga
     1561 aggggacgac caacaggttg aacagcttct tggcgaagaa gaagcagacg aggaaagcgg
     1621 cgaagaagcc gccgatcgac cagatcaacc gccgccgcaa ctcgatcaga tgctccatga
     1681 gcggagcgga ggatttttcg atctcgtcct gttcgtcgac gctcacttcg cggtacctgt
     1741 cgtcttttta ttggccgtta ccggcttttt cgcgggcgtc gaagcggact tttttgctat
     1801 cgcagccggc ttggccgggg cctgcttcgc cgcggctttt gccggcacgg ccttggccgg
     1861 ggccggtttt gcggcgacag gctttgccgg cggagctttg ttagcaacgg gcgtcgacct
     1921 ggcagccaac ggcacaggcg ccggttttgc agacgcagcc ttcaccggcg cggatttcgc
     1981 gggagccgac cttgcggctg gcttggcggc ctccgtcttg gccgatgcgg gttttgccgg
     2041 cgcaggcttt gcgggggctg gctttgcggc tttcggcgcg gctatggtag cttttttggc
     2101 agccggaacc ttcgccggtt cggcggcggg aactgacccg gtcaccaccg aggcatcggt
     2161 catcgccgga taagcgggcg tcgaggctgg cgcgtcggat tcaggaatgc ccagcatgtc
     2221 ggcgccggca ttcttcagcg gctccgccgc ggacgcttcg gccgccgccg gttcggacgg
     2281 gtcggcggcg ggtttcggct tcatcacatt gtcgaggccg gatcgcacgt cggcggcggc
     2341 cttttcgaac ggattgagct gctttcttat ctcggcggcg ggattgaggc cgcgcaacgc
     2401 atcgaccgat ttcttgacgt cgtcaagctc ggcttctttc agcgcgtcgt tgaactgctt
     2461 ttgaaaatcg cccgccatgg cgcgcagctt ggccaccgtg cggccgaagg tgcgcagcat
     2521 attgggaagg tccttcggcc cgacgacgac gatcatgacg accgcaatca ccagcatctc
     2581 ggtccagccg acttcaaaca tggctattca atccgatcag gcgcggtgga aaccgctcag
     2641 ctcttgctgg ctttttcctt ggcggcggcg acggtttcgt cggcgcgatg ctccacggtg
     2701 cgcttgtcgt cggcgacatc gtcgtcggcc atgcccttct tgaagctctt gatgcccttt
     2761 gccatgtcgc ccatcagctc cggaatcttg ccgcggccga acacaagcag gacgatcacg
     2821 agcacgatca tccagtgcca aatcgaaaac gaacccattg caactctctc tcgaatgttt
     2881 cccgttcggg tgatttatgt gttttcgtgt tcggtttcaa acacaactat cacagtctta
     2941 gcggaagcat catgcatgtc acgcggtttc gtcggctata aggcgcgctg cgtcaatctt
     3001 ccgtgacgcg cggggtgagc aggccgagtt cctcaaggtc gatatccgtc agcggatcct
     3061 cgtcctcgtc cagcgcgtcc ggatcggaag gcggcaaagg aatcgagaag ttcgacggca
     3121 tgcggcccga aagcaggccc gcccccttca attcctccat gccgggaaga tcacggattt
     3181 cctccagacc gaaatgatcg aggaaggcat cgctcgtgcc ataggtgacg gggcggcccg
     3241 gaacccggcg gcggccgcgc atgcgcaccc aatccgtctc catcagcgtg tcgagcgtgc
     3301 ccttggaagt ctcgacgccg cgaacttcct cgatctcggc gcgcgtcacc ggctggtgat
     3361 aggcgatgat ggcaagaact tcgagcgcgg cgcgcgaaag cttgcgctgc tgcaccgtgt
     3421 cgcggctcat caggaaggcg aggtcgcccg ccgtgcggaa agcccaggca tcgccgacgc
     3481 gcaccagatt gacgccgcgc cgcgtataga cctgctgcaa ttgcgccatg acggcgtcga
     3541 tgtcgatgcc atcgggcagt ctggcggcca gttgccttgc gctgaccggt tcggcgctgg
     3601 cgaagacgat cgcctcggcc atgcgcacgg cctcagccag atgcagccgc tgcatggtgc
     3661 tcttgccatc ggcggcagcc tcgtcgtccg ccttgaacgg aagaacggat gcacccgtgc
     3721 tttcgctcat gatggaccct cgaaggatgc cgggcgctgg cggccacgca ggtagatcgg
     3781 cgcgaaagcc tcctgctgcc tgacttccag cgtgccttcg cgcaccagtt cgagcgaggc
     3841 ggcaaacgaa ctggcggtgg cggtgcggcg ctcctgcggc gtggcgagat attcgatgag
     3901 gtactggtcg aggtcggtcc agtcggccag cgagccaacc agcctggtca gcatgtcgcg
     3961 cgcctgtttc aacgaccaca cgccgcgccg ggcgatgcgc acattggtga tcgcctgttt
     4021 ctggcgctgg atggcatagg cggtcagcag atcgtaaagc gaggcggtaa aggcgttgcg
     4081 cttttcgacg atgaccattt ccggcatgcc gcgcgcgaag acatcgcggc caagccggtt
     4141 gcggttgacc agccgggcgg cggcatcgcg catggcttcc agccgcttca ggcggaactg
     4201 gagcaccgcc gccagttcct cgccgctctc gccctcgtcg ccgggctgtt tggggatcag
     4261 gagcttcgat ttcaggaagg ccagccacgc cgccatcacc agatagtcgg cggcaagctc
     4321 cagccgaacc gcgcgcgcct ggtcgatgaa gaccagatat tgctcggcca gcgccaggat
     4381 ggaaatgcgc gacaaatcca ccttctggtt gcgggcaagg tgcagcaaca ggtcgagcgg
     4441 gccttcgaaa ccggcgacat cgaccaccag cgacggatcg cccgtcaggc gcgaatcctc
     4501 gccctccgcc cagatggctt ccatcggggc ggatttgcct ggcttttgcg tccctgtctg
     4561 ccctgtcgcc gtctgctttc tcccctaggc taccaccgcc tcgaaatagg tcgcgaattc
     4621 ttcgcggata tcagcttcct ttaccgcatc ggccttgccg atgacagcaa gcgcctttcg
     4681 cgcgcgttcc agcgccgtgc cttccagacc ggtggtgcgc gaggcgatgc ccgccatttc
     4741 ctcgaaaacg ccgttgcagt gaaggacgag atcgcagccc gccgcaagga tggaggcggc
     4801 ctttgtcggg aaatccccag aaagtgcctt catcgaggta tcgtcgctga tcaaaagccc
     4861 gtcgaagccg atttcgcggc ggataatttc ctcgatgacc ttgccggagg tcgtcgccgg
     4921 attgttcgga tcgacggcgc tgaagatgac atgcgcggtc atcgccgccg gcagatgcgc
     4981 aagctggcgg aacggctcga aatcgtggcg gcgaagctcg tccagcttgg catcgacggt
     5041 gggcagttcg aaatgcgtgt cggaaaatgc ccggccgtgg cctggaatat gcttcatgac
     5101 cggcagaacg ccgccggcca tcaggccctc cgccgcggcg cggcccagtt cggtgacgct
     5161 tattggctcc ttgccgtagg cgcgcgtgcc gataacgtcg ctcgcccctt cgatgggtac
     5221 gtcgagcacc ggcaggcaat cggcggtcag atcgtagctt ttcaggtcga aggcgtgcag
     5281 gcggccaagc agccaggcgg cgcggacgcc ggcatcatgg tcgtcgcgcc acaaagcgcc
     5341 aagggccgcg cctgccggat aattgggcgc gagcggcggg cgcaggcgct gcaccctgcc
     5401 cccttcctga tcgacgaata ccggcgcatc gggcctgccg atgcaatcgc gcatcgaggc
     5461 gaccagatcg cggatttgtt ccggctcgct gatgttgcgg gcaaaaagaa tgaagcccca
     5521 cgggcattcg ccccgataga agcggatttc ctcgcgggtg agcgatttcc cggcgcagcc
     5581 gaggatcatg gattttgatt cggtcatggc cctactctaa agcgttccag cgacgaaagg
     5641 aatcggctcg cgcaatggct ggtcagtcgg caatttgccc gaccagggcc ggcaattcct
     5701 tgaggaagtt ttcgcgcacc agcttgccgc cgccctttcc gttctcgtct ttctgctcaa
     5761 gccgggcaaa gacga
//
LOCUS       DASYCS010000250         2854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_350209,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000250 DASYCS010000000
VERSION     DASYCS010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350209"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /gene="tyrS"
                     /locus_tag="VGM46_08105"
     CDS             complement(<1..497)
                     /gene="tyrS"
                     /locus_tag="VGM46_08105"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEY4192597.1"
                     /translation="MFAFKSDFLRTMSERGFIHQTSDDAGLDKLFATESVTAYIGFDA
                     TAKSLHAGSLIQIMMLHWLQKTGHRPIALMGGGTSMIGDPSFKDEARKMLTPQDIADN
                     LVGIRRNFAPYIKFGDGPGDAVMANNADWLMGINYVNFLRDVGRHFSVNRMLSFDSVK
                     LRLDR"
     gene            601..1725
                     /locus_tag="VGM46_08110"
     CDS             601..1725
                     /locus_tag="VGM46_08110"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEY4192598.1"
                     /translation="MKAICAIGLMSGTSMDGIDLAMLKTDGEDFVERGPAFFVPYEAD
                     FRRRIEASLEEAKQIVRRQERPGDLAAVEREITDRHARAIAAFLATAAKDWGQPALVG
                     FHGQTVLHRPHLGLTVQLGDGGRLAALSGLPVVYDMRANDMVHGGQGAPLVPAYHAAL
                     ARTLPAPFAGKYPVVFVNIGGISNITYVPETGDPVAFDTGPGNALIDQWVSREGGIPF
                     DAGGAIASEGGVVAAVVARYLDKPFFARPGPKSLDRNDFTLAEAQGLELADGARTLAA
                     VSAEAILKSVEHLPEKPKLWIVCGGGRKNPHIVGDLRIGAEKAEAEVILAEDAGLDGD
                     ATEAEAWAYLAVRARKGLPLTFPTTTGCSEAVTGGIIAHG"
     gene            1886..2635
                     /locus_tag="VGM46_08115"
     CDS             1886..2635
                     /locus_tag="VGM46_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY4192599.1"
                     /translation="MPDVSTSPISTSIVIPCRNEAENLPLLLDEIETAMAGRAFEVIV
                     VNDGSTDDTAAVLAAQAAKRAFPLRQIRHEKSAGQSLSLRSGAWAARGEIIATIDGDG
                     QNDPQYLPVLVDALRNAGPGAGAAQGQRLKRRDSRAKQMASRFANWLRNAILHDETRD
                     TGCGLKAIHADILRKLPFFDGTHRFVPALVIQEGFGVVHCDVIDRSRRHGKSNYGIFD
                     RGLQGALDLMGVWWLRRRRRRVPKIGEVHHD"
     gene            2628..>2854
                     /locus_tag="VGM46_08120"
     CDS             2628..>2854
                     /locus_tag="VGM46_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006467250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HEY4192600.1"
                     /translation="MTDVLTPLVTWLHQVFVQQFDGWVLLGFVAQAFFTMRFIVQWLA
                     SERAKRSVVPIAFWFFSLFGGGLLLIYAIQRR"
BASE COUNT          493 a          899 c          923 g          539 t
ORIGIN      
        1 tcgcggtcga gccgcaattt caccgaatcg aaggagagca tccggttgac ggaaaaatgc
       61 cggccaacgt cgcgcaggaa gttgacgtag ttgatcccca tcagccagtc ggcattgttg
      121 gccatcaccg cgtcgccggg accgtcgccg aacttgatgt aaggcgcgaa gttgcggcgg
      181 atgccgacga gattgtcggc aatgtcctgc ggcgtcagca ttttgcgcgc ctcgtccttg
      241 aacgagggat cgccgatcat cgaggtgccg ccgcccatca gcgctatggg ccgatggccg
      301 gtcttttgca gccaatgcag catcatgatc tggatcagcg aaccggcatg caggcttttc
      361 gccgtcgcgt cgaacccgat ataggcggtc acgctctccg tggcgaaaag cttgtccagc
      421 ccggcatcgt ccgatgtctg gtggatgaag ccgcgctcgc tcatggtgcg caggaaatcg
      481 gatttgaagg cgaacatttt tctatcccgg aaaatcaccc ggcttttttg ccaagccgtg
      541 gcatgtatgg tcgcgcgcgt ttagcatctt gcgaggcgga atcacaagaa cggcggcatc
      601 atgaaggcaa tctgcgctat cgggctgatg agcggcacgt cgatggacgg catcgacctg
      661 gccatgctga agacggatgg cgaggatttt gtcgagcgcg gcccggcctt cttcgttccc
      721 tatgaggccg atttccgccg ccgcatcgaa gcctcgctgg aagaggccaa gcagattgtg
      781 cggcggcaag aacgaccggg cgatctggcc gcggtggaac gcgaaattac cgaccgccac
      841 gcccgcgcca tcgccgcgtt cctggccacc gccgccaaag actggggcca gccggccctt
      901 gtcggcttcc atggccagac ggtgctgcac cgcccgcatc tcggccttac cgtccagctt
      961 ggcgatggcg ggcggctggc ggcgctgagc ggactgccgg tggtctacga catgcgggca
     1021 aacgacatgg ttcatggcgg gcagggcgcg ccgctggtgc cggcctacca tgcggcgttg
     1081 gcgcgaacgc tgcctgcgcc gttcgccgga aaatatccgg tcgttttcgt caatatcggc
     1141 ggtatttcca acatcaccta cgttcctgaa accggtgatc cggtggcttt cgataccggc
     1201 cccggcaacg cgctgatcga ccagtgggta tcgcgcgagg gcggcattcc cttcgacgca
     1261 ggcggcgcaa tcgccagtga aggcggcgta gtggccgccg tggtcgcccg ctatctcgac
     1321 aaaccgtttt ttgcccggcc cggcccgaaa tcgctcgacc gcaacgactt tacgcttgcc
     1381 gaggcgcaag gtttggaatt ggccgatggc gcgaggacgc tggccgccgt gtcggccgag
     1441 gcgatcctca aatccgtcga gcacctgccg gaaaaaccta aattgtggat cgtctgcggc
     1501 ggcggccgca agaacccgca tattgtcggt gacttgcgca tcggcgctga aaaggccgaa
     1561 gctgaggtca tccttgccga agatgccggg ctggacggcg acgccaccga ggcggaggcc
     1621 tgggcctatc tggcggtgcg cgcccgcaag gggctgccgc ttaccttccc gacgacgaca
     1681 ggttgtagcg aagcggtgac gggcggcatc atcgcgcatg gctgaaccga cgcattgcgc
     1741 cgggcgatga cgggtcgtct tgcgcggctt tgcgggcggg cgtattgagg cgccgcgcag
     1801 gcaggcttcg aaagctcgcc tgccggcccc tcggatcggc tgcgaggccg gcggagcgtt
     1861 tcgtaacgac agagttccaa gcgacatgcc tgacgtatcc acatccccca tatcgacatc
     1921 gatcgtcatt ccgtgccgca acgaggccga aaatctgccg ctcctgctcg atgagatcga
     1981 gacggcgatg gccgggcgcg ccttcgaggt gatcgtcgtc aatgacggct ccaccgacga
     2041 caccgccgcc gtgctggcgg cacaggctgc aaagcgcgcc tttccgctgc gccagataag
     2101 gcatgagaag tcggccggcc aaagcctctc cttgcgttcc ggtgcgtggg cggcgcgcgg
     2161 cgaaatcatc gcgaccatcg acggcgacgg ccagaacgat ccgcaatatc ttcccgtcct
     2221 tgtcgatgcg ctgcgcaacg caggccctgg cgcgggcgcg gcgcaaggcc agcgcctgaa
     2281 gcgccgcgat tcgcgcgcca agcagatggc ttcgcgcttc gccaattggc tgcgcaacgc
     2341 catcctgcac gacgaaacac gcgataccgg gtgcggcctc aaggcgatcc atgccgacat
     2401 cttgcgcaag ctgcccttct tcgacggcac ccaccgtttc gtcccggcat tggtcatcca
     2461 ggaaggattt ggcgtcgttc attgcgacgt catcgatcgg tcacgtcgcc atggcaaatc
     2521 gaattacggc atcttcgatc gcggcttgca gggcgcgctc gacctgatgg gcgtatggtg
     2581 gttgcgccgc cgccgccgcc gcgtgccgaa gattggggaa gttcaccatg actgacgtgc
     2641 tcaccccatt ggtgacgtgg ctgcatcagg ttttcgtgca gcagttcgac ggctgggtgc
     2701 tgctgggttt cgtcgcgcag gcgttcttca ccatgcgttt catcgtgcag tggctggcct
     2761 ccgaacgcgc caaacgcagc gtcgtgccga tcgccttctg gtttttttcg ctgttcggcg
     2821 gcggcctgct gctgatctac gccattcagc ggcg
//
LOCUS       DASYCS010000251         2743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3542538,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000251 DASYCS010000000
VERSION     DASYCS010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3542538"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..1051)
                     /locus_tag="VGM46_08125"
     CDS             complement(59..1051)
                     /locus_tag="VGM46_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY4192601.1"
                     /translation="MKRFIALLFVVVAAHAAQAQQLDKVRFGTNWVAEAEHGGFYQAL
                     ADGTYKKYGLDVTIVPGGPNVNNRILLPVGKIDFFMSGNSLQSFDAVANNIPTVAVAA
                     SFQKDPQVLIAHPGTAGKFEDLKKLTLFVSKEGMPTYFQWLKSDYGFDESEVKPYTSN
                     AQPFLADKNSAMEGYVTSEPYAIEKQAGFKPKVFLLADYGFNSYSTLIETRRDLVEKR
                     PDLVQRFIDASAIGWYHYIYGDSRSGNALIKQQNPEMTDGQLAYSIAKMREDGIVDSG
                     DTAKLGIGAMTDAHWKSIFDKMTHAGVVKPGLDYKRAYTLQFVNKGVGVELRPK"
     gene            1236..2003
                     /locus_tag="VGM46_08130"
     CDS             1236..2003
                     /locus_tag="VGM46_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192602.1"
                     /translation="MLDTSRSRPPAAWSAGKCATARRPAMQMLFALVAAMAPAAAEAA
                     GGAFVVDDAEIGKPGDCKVESWVAFASNHDFQAVTQPACVANLGIPVELTATYARTRT
                     DGEWQNFAGGKAKINLVPVETGRIGIGLVESALWDVRSGQNNFNMLFVPLTYQLSDKF
                     RININAGWQYDGSHQANQNYWGAGFEWNFQQPLTLIGEVFGFAGPHVDPTSFTEPRAQ
                     LGLRVTPVKNLDVDLIYGRNVGGENANWFTLGLNVRF"
     gene            complement(2036..>2743)
                     /locus_tag="VGM46_08135"
     CDS             complement(2036..>2743)
                     /locus_tag="VGM46_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEY4192603.1"
                     /translation="GAIAASFMGYNLAKRFSKDPESFGKGRIEGVFAPETAAHASGTA
                     ALLPMLALGIPGSGTAAILLGGLMVWGLNPGPLLFVEHKDFVWGLIASMYLGNIVGLV
                     MVLSTVPVFAAVLRVPFAAIAPMIVVSCAIGAYAIQNAMFDIWLMLVFGVVGYVFKKV
                     GIPLAPFTLALVLGSRAEDAFRLSMIGADGDLRVFWSNPLVGSITTLAIILLFWPVIG
                     RVVSAAMAQVRGKPATQ"
BASE COUNT          548 a          881 c          822 g          492 t
ORIGIN      
        1 cgcaagcggg aatccagcct acctcaagcg tgtcgcgcgg caatgccatt cgatgggtct
       61 acttcggcct caactccacg cccacaccct tgttgacgaa ttgcagcgta taggcgcgct
      121 tgtaatcgag gccgggcttg acgacgccgg catgcgtcat cttgtcgaaa atgctcttcc
      181 agtgcgcgtc ggtcatggcg ccgatgccaa gctttgccgt gtcgccggaa tcgacgatgc
      241 catcctcccg catcttggcg atggaatagg cgagctggcc atcggtcatt tccgggttct
      301 gctgcttgat cagcgcattg cccgaccggc tgtcgccgta aatgtagtga taccagccga
      361 tggccgaggc atcgatgaaa cgctgcacga gatcgggccg cttctccacc agatcgcggc
      421 gcgtctcgat caaggtcgaa tagctgttga agccgtaatc ggcgagcagg aacaccttcg
      481 gtttgaaacc ggcctgcttc tcgatggcat aaggttcgga agtgacatag ccttccatcg
      541 ctgaattctt gtccgcgagg aacggctgcg cgttcgaagt gtagggtttc acctccgact
      601 catcgaagcc gtaatccgac ttcagccact ggaaataggt gggcatgccc tccttggaca
      661 cgaagagcgt cagcttcttg aggtcttcga attttcccgc cgtgcccgga tgcgcgatca
      721 gcacctgcgg atccttctgg aagctcgcgg ccaccgcgac ggtcgggatg ttgttcgcga
      781 ccgcgtcaaa ggactgcagc gaattgccgc tcatgaagaa gtcgatcttg cccacgggga
      841 gcaggatgcg gttgttcacg ttcggcccgc ccgggacgat ggtcacgtcc aggccgtact
      901 tcttgtacgt gccgtcggcg agcgcctgat agaagccgcc atgctccgcc tcggccaccc
      961 agttggtccc gaaacggacc ttgtcgagtt gctgcgcctg cgccgcgtga gcggcaacaa
     1021 cgacgaatag aagcgcgata aatctcttca tctgctcact atgtcatggg ctggtccgat
     1081 gatccggcca cagttccatg attcccgttt cacaccccaa ggcggcgtaa cttcttttta
     1141 ttaattccaa cttgcgaaaa agcatctcca gtggtgtgat cagcccagtt taggatgatt
     1201 cagcacccgc aaggttccga aaaagccgat caacaatgct cgacacgtct cgctcgcggc
     1261 ctccggcggc atggagcgcg ggaaaatgtg ccacggcgcg tcgcccggcc atgcaaatgc
     1321 tctttgcatt ggtggcggcc atggctccgg ctgccgccga agccgccggc ggcgccttcg
     1381 tcgtcgacga cgcggaaatc ggcaagccgg gcgactgcaa ggtcgagtcg tgggtcgcct
     1441 tcgccagcaa ccacgacttc caggccgtca cgcagccggc atgcgtcgcc aatctcggca
     1501 tcccggtcga actgaccgcc acctatgcgc ggacacgcac cgatggcgag tggcagaact
     1561 ttgccggcgg caaggccaag atcaatctgg tgccggtcga aacgggccgc atcggcatcg
     1621 gcctggtcga gtcggcgctg tgggacgtgc gcagcggcca aaacaacttc aatatgctgt
     1681 tcgtgccact cacgtatcag ctgagcgata agttccgcat caacatcaat gccggctggc
     1741 aatatgacgg ctcccatcaa gcgaaccaga actattgggg cgccggcttc gaatggaatt
     1801 tccagcagcc gctcaccttg atcggcgaag tcttcggatt cgccggcccg cacgttgacc
     1861 cgacgagctt caccgagccg cgcgcgcagc tcggcttgcg cgtgacgccg gtgaagaacc
     1921 tggatgtcga cctcatttac ggccgcaacg tcggcggcga aaacgcaaac tggttcacgc
     1981 tcgggctcaa cgtgcggttt tagcgcggag gcggcccggc aatggccaaa acggactact
     2041 gcgtcgccgg cttgccgcgc acttgcgcca tcgccgcgga caccacgcgc ccgatcaccg
     2101 gccagaacag caggatgatg gccagcgtcg tgatcgaccc gaccagcggg ttggaccaga
     2161 acacgcgcag gtcgccatcc gccccgatca tcgagaggcg gaacgcatcc tcggcgcggc
     2221 tgccgagcac cagcgccagc gtaaacggcg ccagcggaat gccgactttc ttgaacacat
     2281 agccgacgac gccgaacacc agcatcagcc agatgtcgaa catcgcgttc tggatggcgt
     2341 aggcgccgat cgcgcaggac accacgatca tcggcgcgat ggcggcgaag ggcacgcgca
     2401 gcaccgcggc aaacaccggc acggtgctca acaccatcac caggccgacg atgttgccga
     2461 gatacatcga ggcgatgagg ccccagacga aatccttgtg ttcgacgaac agcagcgggc
     2521 cgggattgag cccccacacc atcaagccgc cgagcaggat cgccgccgtg ccggagccgg
     2581 gaatgcccag cgccagcatc ggcaacaagg ccgccgtgcc ggaggcatgc gccgccgttt
     2641 ccggcgcgaa cacgccctcg atgcggcctt tgccgaagct ttccgggtcc ttcgaaaacc
     2701 gcttggcgag attgtagccc atgaacgagg cggcgatcgc gcc
//
LOCUS       DASYCS010000252         5866 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2672047,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000252 DASYCS010000000
VERSION     DASYCS010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 5866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5866
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2672047"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VGM46_08140"
     CDS             <1..309
                     /locus_tag="VGM46_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015317721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY4192604.1"
                     /translation="VLGVISFATVRFQGISLNWQAPAPWLFVAGGMLGGFYVTLSTIL
                     IPRIGAAALMAFLVAGQLLAGMMLDRIGFLGMATREISLGRIAGAVLLLGGALLIRLF
                     "
     gene            complement(328..972)
                     /locus_tag="VGM46_08145"
     CDS             complement(328..972)
                     /locus_tag="VGM46_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY4192605.1"
                     /translation="MTKVKVCGFSVSLDGFAAGPEQSLADPLGKRGPELFQWFFGTRT
                     FRAMTGKEGGSSGPDERFAAASMAGFGAFILGRNMFGPVRGEWPDDTWKGWWGDNPPY
                     HAPTFILTNHPRAPIEMDGGTTFHFVTEGIQAALEQAKAAAGGLDVKIGGGVSTVRQY
                     LVDGSIDEMHLAISPVVLGRGEALFAGLDLPAMGFSVVQSVASDHATHVVLARR"
     gene            1052..2128
                     /gene="queA"
                     /locus_tag="VGM46_08150"
     CDS             1052..2128
                     /gene="queA"
                     /locus_tag="VGM46_08150"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016733044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEY4192606.1"
                     /translation="MKVNLFDFDLPEERIALRPAVPRDSARLAVVKPGRSVEDRIVHD
                     LPSLLKAGDVLVFNDTRVLPAQLKGTRWRGEAAAQVEATLHMRTTPDSWLAFMRPGKR
                     IAVGDRIQFGHDGNACFLGQLNATVLEKREGGEILLGFDVSGPFLDEALHAVGHIPLP
                     PYIASKRDDDERDLSDYQTIYANEEGAVAAPTAGLHFTPELFAALDKAGVERRFVTLH
                     VGAGTFLPVKVDDTADHKMHSEIGVVSTEVAEALNAAKARGGRIVAVGTTSLRLLESA
                     AGDDGQLAAWSGPTDIFITPGYRFKAIDMLMTNFHLPHSTLFMLVSAFSGLETMRAAY
                     ARAIETGYRFYSYGDASLLFRQDR"
     gene            2125..3255
                     /gene="tgt"
                     /locus_tag="VGM46_08155"
     CDS             2125..3255
                     /gene="tgt"
                     /locus_tag="VGM46_08155"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEY4192607.1"
                     /translation="MTAEFSFKLFATDGKARRGEISMPRGEIRTPAFMPVGTGGTVKT
                     MYMDQVHDLGADIILGNTYHLMLRPGAERVARLGGLHEFARWPHPILTDSGGFQVMSL
                     SKLRKLTEKGVTFRSHIDGAAYEMTPERSIEIQGLLDSDIQMQLDECTALPAERKEIE
                     RAMELSLRWADRCKAAFGDQPGKAMFGIVQGGDVADLRVRSAQALAAMDLKGYAVGGL
                     AVGEPQAVMLEMLDITCPELPAAKPRYLMGVGTPDDILKSVARGIDMFDCVMPTRAGR
                     HGLAYTRRGKVNLRNARHADDHRPLDEESDCPAARDYSRAYLHHLVRSQEALGAMLLT
                     WNNLSYYQKLMQDIRAAIEAGAFEARGAEISQGWAQGDIPPL"
     gene            3409..3645
                     /locus_tag="VGM46_08160"
     CDS             3409..3645
                     /locus_tag="VGM46_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008875966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEY4192608.1"
                     /translation="MRAFFVQIKCELGKAYEVASALADAEIASEIYSTAGNYDLIAKF
                     YVEDEEDVGHFVNEKVHALPHIKDTFTTVTFRAF"
     gene            4115..4882
                     /locus_tag="VGM46_08165"
     CDS             4115..4882
                     /locus_tag="VGM46_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEY4192609.1"
                     /translation="MYLPPRHSEIIQMAKDNGRVLVDDLATHFNVTPQTIRKDLNDLC
                     GQRLLSRIHGGAVFPSGIENMQYEARRKIAADEKEAIGRAAAALIPDNASLFVNIGTT
                     TEAVSKALLDHHGLMVITNNINVANRMRVYPSIEVVIAGGVVRGSDGGIVGEAAVDFI
                     KQFKVDYAVIGASAIDHDGALLDFDFREVKVAQAIIANARHVILVSDSTKFDRTAPVR
                     IGHLSQVDTFITDACEIASIRRICEEAEVTLVETASG"
     gene            5000..>5866
                     /gene="glpD"
                     /locus_tag="VGM46_08170"
     CDS             5000..>5866
                     /gene="glpD"
                     /locus_tag="VGM46_08170"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015317732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HEY4192610.1"
                     /translation="MSTAPVHDIFVIGGGINGCGIARDAAGRGFSVFLAEMNDLASGT
                     SSGSTKLIHGGLRYLEFYEFRLVREALMEREILWRNAPHIIWPMRFVLPYAKGLRPAW
                     LLRLGLFLYDHIGGRKLLPPTKTLDMRSDPAGKPLKPLFQKAFEYSDGWANDARLVAL
                     NARDAADRGATVRTRTKVVAARREGELWSITLQDMIAGGTEEVKARLLVNAAGPWVDH
                     VLSGVVGRQDVHNVRLVQGSHIVVRKKFEDPRAYFFQNKDGRIIFAIPYEEDFTLIGT
                     TDRDFDGDPHAVK"
BASE COUNT         1093 a         1811 c         1802 g         1160 t
ORIGIN      
        1 gtgctcggcg tgatctcgtt tgcgactgtc cggttccagg gcatttccct gaactggcag
       61 gcgccggcgc catggctatt cgtcgccggc ggcatgctgg gcggcttcta cgtcaccctt
      121 tccaccattc tcatcccgcg catcggtgct gcggcgctga tggcgtttct ggttgccggc
      181 cagctgctgg ccggcatgat gctggatcga atcggctttc ttggcatggc gacgcgcgaa
      241 atttcgctcg gacgcatcgc gggcgccgtt ctgttgctgg gcggggcctt gctgatccgg
      301 ctgttctgaa cccctgccgg cggcacttca acgcctggcc agcaccacat gggttgcgtg
      361 atcgctcgca acgctttgca cgaccgaaaa tcccatcgcg ggcaaatcca gtccggcaaa
      421 caacgcttcg ccacgaccaa gaaccacagg cgaaatcgcc aggtgcattt catcgatgct
      481 gccatcgaca agatattgcc gcaccgtcga gacgccgccg ccaatcttga catcgagacc
      541 accggcggca gctttcgcct gctcaagggc ggcttggata ccctcggtga cgaagtggaa
      601 agtggtgccg ccgtccattt cgatcggcgc gcgcggatgg ttcgtcagga tgaaggtcgg
      661 cgcgtgatag ggcgggttgt cgccccacca gcctttccat gtgtcgtcgg gccattcccc
      721 gcgaactggc ccgaacatgt tgcggccaag gataaaagca ccaaaacctg ccatcgaagc
      781 ggcggcaaag cgctcgtcgg ggccggagga gccgccttct tttccggtca tggcgcgaaa
      841 tgtccgggtt ccaaaaaacc actggaacag ctccgggcct cgtttgccga gcggatcggc
      901 caggctctgc tccggcccgg cggcgaagcc atcgagagaa actgaaaagc cgcagacttt
      961 cactttggtc atcggataag ccccgtgtct ggaaacgccg cgacaggaat gacagtagcg
     1021 gtgcggccaa gatcaggata ttgccgctgc catgaaggtc aacctgttcg atttcgattt
     1081 gccggaggag cgcattgcgc tgcgccccgc cgtgccgcgc gacagcgccc gcctggctgt
     1141 cgtaaagccg ggccggagcg tcgaagaccg gatcgtccac gatctgccgt ccctgctcaa
     1201 ggccggcgac gtgttggtgt tcaacgacac cagggttctg ccggcgcagc ttaaaggtac
     1261 gcgctggcgc ggcgaggcgg cggcgcaggt cgaggcgacc ttgcatatgc gcaccacgcc
     1321 cgacagctgg ctggccttca tgcggcccgg caagcgcatt gcggtcggcg accgcattca
     1381 gttcggccat gatggaaacg cctgctttct cggccagttg aacgccacgg ttctcgaaaa
     1441 gcgcgagggc ggcgagatat tgctcggctt cgatgtttcc gggccgttcc tcgatgaagc
     1501 gctgcacgcc gtcggccata ttccgctgcc gccctacatc gcctccaagc gtgacgacga
     1561 cgaacgcgac cttagcgact accagacgat ctacgccaat gaggagggcg cggtcgccgc
     1621 gcccaccgcc ggcctgcact ttacgccgga gcttttcgcg gcactggaca aggcgggcgt
     1681 cgagcgccgt ttcgttaccc tgcatgtcgg cgcgggcacc ttcctgccgg taaaggtgga
     1741 cgacaccgcc gaccacaaga tgcactctga aatcggcgtc gtttcaacgg aagtggccga
     1801 ggcgctgaat gcggcgaagg cgcggggcgg gcgcatcgtc gctgtcggca ccacctcgct
     1861 gcgcctgctg gaaagtgcgg ctggcgacga cggacagctt gcggcgtggt ccgggccgac
     1921 cgatattttc attacgccgg gctaccggtt caaggcgatc gacatgttga tgaccaattt
     1981 ccatctgccg cattcaacgc ttttcatgct ggtttcggct ttttcggggc tggaaaccat
     2041 gcgcgccgcc tatgcgcgcg ccatcgagac gggctatcgc ttctattcct atggcgatgc
     2101 cagcctgctg ttcaggcaag accgatgacg gcggagttct cgttcaagct gttcgccacc
     2161 gacggcaagg cgcggcgcgg cgaaatttcg atgccgcgcg gggaaatccg caccccggcc
     2221 ttcatgcctg tcggcacagg cggcacggtc aagaccatgt acatggatca ggttcacgat
     2281 ctcggcgcgg atattatcct gggcaacacc tatcatttga tgttgcggcc gggtgcggag
     2341 cgcgtggcgc ggcttggcgg attgcacgaa ttcgcccgct ggccgcaccc gatcctgacc
     2401 gatagcggcg gtttccaggt catgtcgctg tcgaaactgc gcaaactgac cgaaaaaggc
     2461 gtcactttcc gctcgcatat agacggcgcg gcctatgaga tgacgcccga gcgctcgatc
     2521 gaaatccagg gcctgctcga ttccgacatt cagatgcagc tagacgagtg cacggcgctt
     2581 ccagccgagc gcaaggaaat cgagcgcgcc atggaactgt cgctgcgctg ggccgatcgc
     2641 tgcaaggcgg cgttcggcga tcagcccggc aaggcgatgt tcggcattgt ccagggcggc
     2701 gacgtggccg atttgcgggt gcgctccgcc caggcgctcg ccgccatgga cctgaagggc
     2761 tacgccgttg gcgggcttgc ggtcggcgag ccgcaggcgg tgatgctgga aatgcttgac
     2821 ataacctgtc ctgaactgcc ggcggccaag ccgcgctacc tgatgggcgt cggcacgccc
     2881 gacgacatat tgaagtcggt ggcgcgcggc atcgacatgt tcgattgcgt gatgccgacg
     2941 cgcgccggcc gccacggcct cgcctatacg cggcgcggca aggtcaacct gcgcaatgcc
     3001 cgccatgccg acgatcaccg tccgctggac gaggaaagcg attgccccgc cgcccgcgat
     3061 tattcccgcg cctacctgca tcatctggtg cgctcgcagg aggccctggg cgcaatgctg
     3121 ctgacgtgga acaatctttc ctactatcaa aagctgatgc aggacattcg cgccgccatc
     3181 gaggccggcg cattcgaggc gcgtggcgcc gaaatctcac aaggctgggc gcagggcgac
     3241 attccaccgc tctagctccg gtttggcagg cgccagaaaa gagtgatggg cggctgcgct
     3301 tgaaagcttg gcggcggtct gcgacaaggg ccgcattggc tcgtcttgtg cccaagagat
     3361 agacgagatg cgtcgccgtt tcgaccgagc gccacaggaa gaaaagtcat gagagcgttt
     3421 ttcgtgcaga tcaaatgcga gttgggcaag gcctacgagg tcgccagcgc gcttgccgac
     3481 gccgagatcg cctccgaaat ctattcgacc gccggcaatt acgacctgat cgcaaagttc
     3541 tatgtcgaag acgaagaaga tgtcggccac ttcgtcaatg aaaaagtgca tgccttgccg
     3601 catatcaagg acaccttcac caccgtcact tttcgcgcct tttaaagcgc gggcgtgacc
     3661 gcaaggctgt gaggcagaga ctgccgccgg ccatatcgat catgctttgg catcagctat
     3721 ttgctgcatc ctcgcgcgac gcgcctataa acccatgatt ggtttgcgga ggcgatgatc
     3781 tgccgctggc gtcgttcgat tcgacgttgg aatcgaaccc ttcgcgccgg ctcgccgtcc
     3841 gatcttgtgt tttaccggaa ggatgcctgc ggggcagggt ctctgaaacg cttgggccag
     3901 gacgaacccg attattgccc gatctccagg cgttggttgg acgcgccgaa gtagcccttc
     3961 cggaaatcgc cggatttcct gccatagcgt cgctttcggc ctgccgtttg gccggttggc
     4021 gaccggcatg ggcacttcgg ggccgatttg cttactgtct tttcgggttt cgcttttttc
     4081 tataagaaga caaacgaaac cggattgcca tgcgatgtat ctgccgcccc gccattccga
     4141 aatcatccag atggccaagg acaatggccg tgtgctggtg gatgacctgg ccacccattt
     4201 caacgtcacg ccgcagacga tccgcaagga tctgaacgat ctgtgcggcc agcggctgct
     4261 ttcgcgcatc catggcggtg cggtgtttcc gtccggcatc gagaacatgc aatatgaggc
     4321 gcgccgcaag atcgccgccg acgaaaagga agctatcggg cgcgccgcgg ctgccttgat
     4381 cccggacaac gcctcgcttt tcgtgaatat cggcaccaca accgaggccg tcagcaaggc
     4441 tttgctcgac catcacggcc tgatggtcat caccaataac atcaacgttg ccaaccggat
     4501 gcgcgtctat ccttccatcg aagtggtgat agcgggcggc gtcgtgcgcg gttccgatgg
     4561 tggcatcgtc ggcgaggcgg cggtcgattt catcaagcag ttcaaggtcg attatgcggt
     4621 gatcggcgct tccgccatcg accatgacgg cgctttgctc gatttcgact tccgcgaagt
     4681 caaggtcgcg caagccatca tcgccaatgc aaggcatgtc atcctggttt ccgacagcac
     4741 caagttcgac cgcaccgccc cggtgcgcat tgggcatctg tcgcaggtag acactttcat
     4801 caccgacgct tgcgaaatcg cttccattcg cagaatttgc gaagaggccg aggtcacgct
     4861 ggtcgaaacc gcatccggtt agcgtatctt ccgcatttcc ggttttgaaa ttcgtttgac
     4921 actctcaaag atttcaaatt aatcagattc gctttcgata aagcgcacag tgaaaaaata
     4981 cgaaattctg gagggctctg tgagcacagc accggtccat gacatatttg tcataggcgg
     5041 cggcattaat ggctgcggca ttgcccgcga tgcggccggg cgtggctttt ccgtgttttt
     5101 ggccgagatg aacgatctgg ccagcggcac gtcttccggt tcgaccaagc tgatccatgg
     5161 cggcctgcgc tacctggagt tctatgaatt ccggctggtg cgcgaggcgc tgatggagcg
     5221 cgaaatcctc tggcgcaacg caccgcacat catctggccg atgcgcttcg tgctgcccta
     5281 cgccaagggc ctgcgcccgg catggctgct gcggctcggc ctgttcctct acgaccatat
     5341 tggcggacgc aagcttttgc cgccgacgaa gacgctggac atgcgcagcg atcctgccgg
     5401 caagccgctc aagccgcttt tccaaaaagc gttcgaatat tcggacggtt gggccaacga
     5461 cgcgcgcctg gtggcgctca acgcgcgcga tgccgccgac cgtggcgcga cggtccgcac
     5521 gcgcaccaag gttgtcgccg cgcggcgtga aggcgaactc tggtccatca cgctacagga
     5581 catgattgcc ggcggcaccg aggaggtcaa ggctcgcctg ctcgtcaatg cggccgggcc
     5641 atgggtcgat catgtgctgt cgggcgtggt cgggcgacag gacgtccaca atgttcgcct
     5701 cgtccagggc agccacatcg tggtgcgcaa gaaattcgaa gatccacgcg cctatttctt
     5761 ccagaacaag gacggccgca tcatctttgc gatcccttac gaggaagatt tcactctgat
     5821 cggcaccacc gaccgggact ttgacggcga ccctcatgcc gtgaaa
//
LOCUS       DASYCS010000253         3795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3687591,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000253 DASYCS010000000
VERSION     DASYCS010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3687591"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..934)
                     /locus_tag="VGM46_08175"
                     /pseudo
     CDS             complement(1..934)
                     /locus_tag="VGM46_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006204757.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
     gene            complement(942..2108)
                     /locus_tag="VGM46_08180"
     CDS             complement(942..2108)
                     /locus_tag="VGM46_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HEY4192611.1"
                     /translation="MTACIVGWAHTRFGKLEGETLENLITTVAAEALDHAGIGPDDVD
                     EIVLGHFNAGFSAQDFTASLVLQADDRLRFKPATRVENACATGSAAVRQGIRAIDAKA
                     ARVVLVVGAEQMTTTPGPQIGENLLRASYLPEEGDTPAGFAGVFGKIASAYFQQYGDQ
                     SDALAMIAAKNHRNGVDNPYAQMRRDFGYDFCRTESEKNPFVAGPLKRTDCSLVSDGA
                     AALVLTDSTTALKMRRAVAFRANEHVQDFLPMSKRDILLFEGCEQAWSRALKNAGVTL
                     DDLSFVETHDCFTIAELIEYEAMGLARRGEGAKLALSGETAKDGRLPVNPSGGLKSKG
                     HPIGATGVSMHVLSAMQLTGEAGGIQVPGAKLGGIFNMGGAAVANYVSILDRIR"
     gene            2328..2921
                     /gene="betI"
                     /locus_tag="VGM46_08185"
     CDS             2328..2921
                     /gene="betI"
                     /locus_tag="VGM46_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006330910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HEY4192612.1"
                     /translation="MPKLGMEPLRRRALIDATISAIGERGSLDVTMSEIAGRAGVSSA
                     LAHHYFGAKDELLLATMRHLLAELGADMKRALRSAETPRARVSAVVAVNFSADQFKPQ
                     TIAAWLAFYVEAQRSMALRRLLRVYARRLHSNLMNGLTAILPRGEADKVAETTAAMID
                     GLYIRRALKDGVPDAATAIALVEDYLHTRLNPKKTMK"
     gene            2918..>3795
                     /gene="betC"
                     /locus_tag="VGM46_08190"
     CDS             2918..>3795
                     /gene="betC"
                     /locus_tag="VGM46_08190"
                     /EC_number="3.1.6.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010914987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline-sulfatase"
                     /protein_id="HEY4192613.1"
                     /translation="MTGQPNILIIMVDQFNGTLFPDGPAEFLHAPHLKALAARSARFQ
                     NNYTGSPLCAPGRSSFMSGQLPSRTGVYDNAAEFVSSIPTYAHHLRTAGYHTCLSGKM
                     HFVGPDQMHGFEERLTTDIYPADFGWTPDYRKPGERIDWWYHNLGSITGAGVAEITNQ
                     MEYDDEVAFHAEQKLFEFSRASDNAARRPWCLTVSFTHPHDPYVARRKYWDLYEDCPA
                     LEPEVGFIPYAEQDAHSKRLYNASDYDSFNITPDQVRRSRQGYFANISYIDDKVGSLL
                     SVLERTRMADDTIILF"
BASE COUNT          704 a         1252 c         1127 g          712 t
ORIGIN      
        1 gcctgggtga tcaaggtcgc ggaaagcgct tcgtcgcttg caagcagctt tgccagaacg
       61 gccgcgatca tcgtttccgc tccccggatt gcgccatcgt cgcatttcag cgcgatgccc
      121 agccccaatt ccggtatggc cgcgcaataa acgccttctg cccccgatta acgaagatgc
      181 gtccaggcgc cgcgcccatc aacgccgcat cggccttatc cgtgcctgaa atcaggaatg
      241 gctcggccat gcaggcgctc accaaacgcc tggcggcttt cgcccgctcc ggcggcaggc
      301 cattgccggt gaccattcgc gcgaagccgc gagcgaggtc gcgcagcggc accgcatagg
      361 tcgggatcga gcaaccgtcg atgccacgat tggcggcgtt atgagccacg ccggttacgt
      421 cttccattgc cgtgcgaacc atttcctgaa acgcatggcc ggccttgacg tagccgccat
      481 gatcgatgcc agcgtgaacg caggtgcata gaaaaccgga atgcttgccc gagcaattat
      541 tgtgcagcgc gcttggcgta ccgccgccct gcgccaacgc cacggtcgca gcctggctgg
      601 acggccagtg ggcgccgcat tcaagcgccg ccgcgtccag cccggcgcgg gccagcatcg
      661 acgccgccag ttccacatgc gccggctcgc ccgtatgcga tgcgcaggcg aacgccagtt
      721 cgcgctggcc gaacccgtag gcgtccgccg cgccggcttc gacaaaaggc agggcctgga
      781 tcacctttac cgccgagcgc ggaaacaccg gccgcgccgt atcgcctatt tcccagacag
      841 gattggcttc accgtcgaac accgcgaccg cgccgcgatg ggcgctttcc accaattcgc
      901 cgcgcaggac ctcaaccaga accggatttg tcatgacgcg ctcaacggat gcggtcgagg
      961 atcgatacgt agttggcgac agccgcgccg cccatgttga agataccgcc tagctttgcg
     1021 ccgggcacct gtatgccgcc ggcttcgccc gtcagttgca tggcgctcaa tacatgcatg
     1081 gaaacgcccg tcgcgcctat cgggtggccc ttggatttca gcccgccaga gggatttacg
     1141 ggcagcctgc cgtctttggc ggtctcgccg ctcaacgcca gtttcgcgcc ctcgccacgc
     1201 ctggccagcc ccatcgcctc atattcgatc agttcggcga tggtgaagca gtcatgcgtc
     1261 tcgacgaagg agaggtcgtc cagcgtgacg ccggcattct tcagcgccct gctccatgcc
     1321 tgttcgcagc cttcgaacag aaggatgtcg cgcttcgaca tgggcaggaa atcctgcaca
     1381 tgctcgttgg cgcggaaggc tacggcccgc cgcattttca gcgccgtcgt cgaatcggtc
     1441 agcacaaggg cggccgcccc gtcggaaacc agcgaacagt cggtgcgttt cagcgggccg
     1501 gcgacaaacg ggttcttctc gctctcggtg cggcagaaat catagccgaa gtcccggcgc
     1561 atctgcgcat atgggttgtc gacgccgttg cggtgatttt tcgcggcgat catcgccagc
     1621 gcatccgact ggtcgccata ttgctggaaa taggcgctgg cgatcttgcc gaaaacaccg
     1681 gcaaaaccgg ccggcgtgtc gccctcttcc ggcaggtagg aagcgcgcaa caggttctcg
     1741 ccgatctgcg ggccgggtgt cgtcgtcatc tgctcggcgc cgacgaccag caccacgcgc
     1801 gcggctttgg catcgatggc gcggatgcct tgccggaccg ccgccgaacc ggtcgcgcaa
     1861 gcgttttcca cgcgggtcgc cggcttgaag cgcagccggt catcggcctg caatacgagg
     1921 ctggcagtaa aatcctgggc ggagaaaccg gcattgaaat gtccaagcac gatctcatcg
     1981 acatcatcgg gaccaatgcc ggcatggtcg agcgcttccg ccgccaccgt cgtgatgagg
     2041 ttttcgaggg tctcgccctc aagcttgccg aagcgcgtat gcgcccagcc gacgatacat
     2101 gccgtcatgg cgatctcctt cgttgcgacg gctttgccgc ctcaattcaa ttccgggcca
     2161 gtccgtggcc aatgtttggc cagtcctggc aaaaaattcc ggtcccctac aaatggacat
     2221 gccgatcggt tccgtaaagc gaagtttgaa gattgggcgc atctggcgca gcagattttt
     2281 gttgattgac cagtcaataa aaaataatct ggccgtggga gatgcacatg cccaagcttg
     2341 gaatggaacc gctgcgccgc agggcgctga tcgacgcgac gatctcggcg atcggcgagc
     2401 gtggctctct ggacgtcacc atgtcggaaa tcgccgggcg cgcgggcgtt tcgtccgcgc
     2461 tggcgcatca ttattttggg gcgaaggacg agcttttgct ggcgaccatg cgccatcttc
     2521 tggcggagtt gggggcggat atgaagcgcg cgctgcgcag cgcggagacg ccgagggcgc
     2581 gcgtttcggc cgtcgtcgcc gtcaactttt ccgccgacca gttcaagccg caaaccatcg
     2641 ccgcatggct cgccttctat gtcgaagcgc agcgttcgat ggcgctgcgc cggctgctca
     2701 gggtctatgc gcgccgcctg cattccaacc tgatgaacgg cctcaccgca atcctgccgc
     2761 gcggcgaagc cgacaaggtc gccgaaacca cggcggcgat gatcgacggg ctttatatcc
     2821 ggcgcgcgct gaaggacggg gtgccggatg ccgccacggc catcgcgctc gtcgaagatt
     2881 atctgcacac caggctcaac ccgaaaaaga caatgaaatg acaggccagc ccaacatcct
     2941 catcatcatg gtggatcagt tcaacgggac gttgtttccc gatgggccgg cggaattcct
     3001 gcatgcgccg cacctgaagg cgctggccgc ccgctccgcg cggttccaga acaattatac
     3061 cggctccccg ctatgtgcgc cgggccgctc atccttcatg agcgggcaac tgccttcgcg
     3121 caccggtgtc tatgacaacg ccgccgaatt cgtctcgtcc attccgacct atgcccatca
     3181 tttgcgcacc gccggctatc acacctgcct gtcgggcaag atgcactttg tcggccccga
     3241 ccagatgcac ggtttcgagg aacggctgac taccgatatc tacccagccg attttggctg
     3301 gacgccggac taccgcaagc ccggcgagcg tatcgactgg tggtatcaca atctgggatc
     3361 gatcacgggc gccggcgtcg ccgaaatcac caaccagatg gaatatgacg acgaagtcgc
     3421 cttccatgcc gagcagaaac tttttgagtt ttcccgcgcg tccgacaacg ccgcgcgccg
     3481 cccgtggtgc ctgaccgttt ccttcaccca cccgcacgat ccctatgtag cgcggcgcaa
     3541 atactgggat ctctacgaag attgccccgc actggagccg gaagtcggct tcatccccta
     3601 tgccgaacag gatgcgcatt cgaagcggct ttataacgcc agcgactatg acagcttcaa
     3661 catcacgccc gatcaggtgc ggcgctcgcg gcaaggttat ttcgccaata tctcctatat
     3721 cgacgacaag gtcggctcgc tgctgtcggt gctggaacgc acgcgcatgg ccgacgacac
     3781 catcatcctg ttctg
//
LOCUS       DASYCS010000254        14071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3831641,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000254 DASYCS010000000
VERSION     DASYCS010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 14071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14071
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3831641"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..138
                     /locus_tag="VGM46_08195"
     CDS             <1..138
                     /locus_tag="VGM46_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEY4192614.1"
                     /translation="GFEVHMGYATARHRAAIDTHGPVPRLHRISFAPFRLSGEEPVAV
                     E"
     gene            complement(369..860)
                     /locus_tag="VGM46_08200"
     CDS             complement(369..860)
                     /locus_tag="VGM46_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEY4192615.1"
                     /translation="MDATSLATLWALIALLIFIGICIYIKVPGMMAKSLDTRGARIRS
                     ELEEARTLREEAQALLAEYQKKRKEAEQEAGAIVEAARREAEILASEAHQRTEDYVAR
                     RTALAEQKIGQAERDAIAEVRSSAVDLAVEAARKLLASHADAKAGADLFKASLQEVKS
                     KLN"
     gene            complement(871..1473)
                     /locus_tag="VGM46_08205"
     CDS             complement(871..1473)
                     /locus_tag="VGM46_08205"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019856925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEY4192616.1"
                     /translation="MFVTPAYGQETAPAVTGVDAQPAGETHAGTEVPHEAAHEGTFPP
                     FDPATFASQIFWLVITFGLFYLFLKRIVMPRLAGIFDTRSSRIFQDLDQANKLKSEAE
                     AAVAAYEQGLAEAKANANAIGQQARDDAKKEAEAARKKVEAGLEAKLTEAEGRIASIK
                     TAAMKDVGAIAESTAGAILEQLVGKSDKAAVSAAVKSVAS"
     gene            complement(1537..1761)
                     /locus_tag="VGM46_08210"
     CDS             complement(1537..1761)
                     /locus_tag="VGM46_08210"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006206606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEY4192617.1"
                     /translation="MDVEAAKYIGAGIACLGMGGAGIGLGNIFGSYLAGALRNPSAAD
                     GQFGRLIFGFAVTEAMGIFSLLIALLALFG"
     gene            complement(1827..2585)
                     /locus_tag="VGM46_08215"
     CDS             complement(1827..2585)
                     /locus_tag="VGM46_08215"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010914826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEY4192618.1"
                     /translation="MAADKVDPIHQFHINKIIPIDLGGYDLSFTNSSLFMVVVVILAG
                     SFFYFATSGRALVPSRWQSSAEMLYEFTASMLKDAAGTQGMKFFPFVFSIFMFVLAAN
                     LLGLFPYFFTVTSQIIVTFTLAMLVIGIVVVYGIAKHGFGFFKLFVPSGVPLVLMPLV
                     ILIEVISFISRPVSLSVRLFANMLAGHITLKVFAGFVVSLSAMGAVGIAGAVMPLAMA
                     VAITALEILVAALQAYVFAILTCMYLNDALHPGH"
     gene            complement(2648..3049)
                     /locus_tag="VGM46_08220"
     CDS             complement(2648..3049)
                     /locus_tag="VGM46_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006206608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEY4192619.1"
                     /translation="MADRKRPGETGETGHGHLSKGDIRDDELERRRRELEASLASRMP
                     EELKGEENAKKGSLAGYGQALKLSSEFIAGIVVGVGIGWMIDRLAGTSPWGLIVFLLL
                     GFCAGILNILRSVGKVAGFGQSGRSTGPKND"
     gene            complement(3267..4457)
                     /locus_tag="VGM46_08225"
     CDS             complement(3267..4457)
                     /locus_tag="VGM46_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010914824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HEY4192620.1"
                     /translation="MHRRVLKRLVAAAASADLKKRSFLSPAIVGLGIWIGFPTVTAYQ
                     DMTSLISGLEAPTRWNAYVEKSVAGSVHAAEMPFVDTLTTGSISGAGVELPGVGSVSF
                     RGKGDAAGTVPDEDRVVRAQKKGRIVQIVPVAPPKNFTAGSIFQRTSSLLRPTMNSGL
                     KLTFAKPAIKGKEIQIAAAFHPTEDKKAAPGVPSMLAALVTNDHADVLATAYAPAEPD
                     YAKASPFEALLQDDKPNEGRFIPPMSKGDHAWLQNPLPASVFSKQEQTCLANAVYFEA
                     RSESLRGQAAVAQVVLNRVRNPAYPASICGVVYQNQNWLNACQFSFACDGHKDRIESP
                     AHYKIARDVAMAVTAGKIFIPEVGSSTHYYAAYVSPGWARAMKKMTKIGLHIFYRTYG
                     GGWS"
     gene            4687..4884
                     /locus_tag="VGM46_08230"
     CDS             4687..4884
                     /locus_tag="VGM46_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192621.1"
                     /translation="MDSPFDIVDEMLGMAKEPAEFSKDDLADMILVAATEIMKLRERM
                     HPPSPAERPERRMPRAFMPQF"
     gene            complement(4918..6354)
                     /gene="der"
                     /locus_tag="VGM46_08235"
     CDS             complement(4918..6354)
                     /gene="der"
                     /locus_tag="VGM46_08235"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019169866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEY4192622.1"
                     /translation="MGFQVAIIGRPNVGKSTLFNRLAGRKLALVDDTPGVTRDRRVHP
                     AKLYDLHFDVIDTAGFEDAAASTLQGRMRAQTEIAIREADLIFFMVDAKAGLLPDDRT
                     FAEIVRKSGKPVVLVANKAEARGAQGGMLEAWELGLGEPIAVSAEHGQGLPDLRDAVV
                     EALGAAMAFGEEEEKDEEIAASEVLIGEDIADPDAEDVPAYDDTKPMRIAVVGRPNAG
                     KSTMINALIGEERLLTGPEAGITRDSISVDWDWRGRKMKLFDTAGMRRKARIQEKLEK
                     MSVQDGLHAIRFAEIVIIVLDATIPFEKQDLQIADLIVREGRAPVIAFNKWDMIERPQ
                     EMLAELREKTERLLPQVRGIQAVPVSAETGRGLDKLMEAVIKTHRIWNSRVSTGKLNR
                     WLEGILAHHPPPAVAGRRLKIKYLTQAKTRPPGFVVSCSRPDAMPQSYVRYLTNNLRQ
                     AFDIPGVPIRMALRTSDNPFAGRAKKRT"
     gene            complement(6354..7013)
                     /locus_tag="VGM46_08240"
     CDS             complement(6354..7013)
                     /locus_tag="VGM46_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006330265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY4192623.1"
                     /translation="MADDSFIREVNEEIRKDQAKAMWDRYGPALLAIALLIIIGTAAY
                     VGYSYWNETRANRSGDAYSQALALADSGKNDDALAAFAKLEQDGYGAYPLLARMRAAT
                     VKADKGDAAGAVKDFDAVAADTAIPAGLRDLARLRAALLLVDHGSYDDVSSRVEALTD
                     DTNPLRHSAREALGLAAWKDGKKADAQKLFDQIVADNAAPQNVRQRATIMAGLIRGSG
                     S"
     gene            7324..8355
                     /locus_tag="VGM46_08245"
     CDS             7324..8355
                     /locus_tag="VGM46_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010914819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HEY4192624.1"
                     /translation="MPSLFRVSKLLCVPTLLFVWALLGIAAPGAARADPPSPLAPAAP
                     KQIVIISFDSARDISQWQRSRALAKRTGAHFTYFLSCVYLLSRDTRKEYTAPHKSAGK
                     SNIGFAPSKEDVTARLEQIRLAAAEGHDIASHACGHFDGKDWSKADWLNEFGSVRRIF
                     ENAYAINGIAPEPTDWRAFAKTAVVGFRAPYLSTGAALYQALQAAGFVYDASGVSRGP
                     ATPETVGAITRFSLPLVPEGPRGKPVIAMDYNLYARHSGAKEQPADARQFEERAYQAF
                     HTAFEAQYAGKRVPLELGFHFTLMNGGAYWNALERFAGEVCVKADVECISFRDYVSRL
                     PKAAPRQDG"
     gene            complement(8362..9627)
                     /gene="sbmA"
                     /locus_tag="VGM46_08250"
     CDS             complement(8362..9627)
                     /gene="sbmA"
                     /locus_tag="VGM46_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008125333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HEY4192625.1"
                     /translation="MFVSFFPQPKLFFTSAVLWSLAAILFWFFGGAELGALIGLPPAA
                     AGAPPIVGIAVFWSKPFLWFYVYFALLVAAFYAFWSWYSPHPWQRWSILVTAVILFLI
                     YFTVQVSVAVNAWYGPFFDYVQGLMSGTTKSTAGEFYRGQASFAWLALVGMNVQVINA
                     FIVSHWIFRWRTAMNNYFMANWGTLRHIEGASQRIQEDTMRFSQIMEDLGSSFVQSIM
                     TLIAFLPVMIGLQQHITELPIIGAVPQPLVVAAIGWCVFGTVLVMVAGIKLPGLQFRN
                     QRVEAAYRKELVYGEDHEDRAQPVTTAQLFDNVRKNYFRLYGHYVYFNVVRYTYLQAD
                     NIFSFVILGPSLVAHKITFGTLNQISNAFGRVTQSLQFLLSSWSTIVELQSVHKRLRA
                     FEATLRGEALPDIDQRYLEREQARAQPVE"
     gene            complement(9758..10921)
                     /locus_tag="VGM46_08255"
     CDS             complement(9758..10921)
                     /locus_tag="VGM46_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006206616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HEY4192626.1"
                     /translation="MKTLFDTQLRFGVAGMIGMAVAMGIGRFVYTPILPGMMEELGLT
                     ASDAGLIASANYLGYLAGALLAAGGWAHGRERLAALCGLGATAILCASMAMTQAMIPF
                     LVIRFFAGVASAFVMVFLTSIVLSHATAGRQGSLQAMHFGGVGLGIAASAGLLAVLVL
                     CHAGWTAGWLWSAVIAACGFLAVAWMADEGPAGSGNGAAEREPPLPKDRSLVKIIVAY
                     GLFGFGYVITATFLITIVRQGGGGRVFETMVWLVTGLAGAPSVWLWNWIAARTGIYAA
                     YALASLIEAVGVAASVSLGGRIGPVLAGLLLGLTFIAATALGLQAGRRHAPQSPRRLF
                     ALMTAAFGVGQIIGPIIAGTLAERSGSFFASSILASAVLVASGALAWSARKTG"
     gene            11064..12320
                     /locus_tag="VGM46_08260"
     CDS             11064..12320
                     /locus_tag="VGM46_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019856914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEY4192627.1"
                     /translation="MTTHMRDFSASIDPVKLDRLAEVAIKVGLQLQPGQDLVLTSSIA
                     ALPLTRKIVEHAYKAGAGLVTPIFNDDEITLARFRYGNDASFDHAAGWLYEGMAKAFG
                     NNAARLAVRGDDPSLLSSQDPAKVSRASKANSMAYQPALEKITGFDINWNIVAYPELA
                     WAKQVFPNETDDVAVAKLADAIFAASRVDVDDPIGNWKAHNGALRSRTEWLNGHNFHS
                     LHFTGPGTDLTVGLADGHEWMGGASTAKNGITCNPNIPTEEVFTTPHAHRISGHVSST
                     KPLSYQGTLIDNISVRFEEGRIVEASASRGEDVLNKVLDTDEGARRLGEVALVPHSSP
                     ISKSGILFYNTLFDENASCHIALGQCYSKCFVDGASLTPDEIAARGGNKSFIHIDWMI
                     GSDKIDIDGVHKDGRRVPVMRKGEWA"
     gene            12327..12683
                     /locus_tag="VGM46_08265"
     CDS             12327..12683
                     /locus_tag="VGM46_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEY4192628.1"
                     /translation="MKIDLTIKRVYDPPAKADGQRVLVDRLWPRGLSKDKAELTLWLK
                     EIAPSTELRKWFDHDPAKWTEFQRRFRAELDESGEAVDELRGLIAKGKVTLLYSAHDE
                     THNNAVALAAYLKEKA"
     gene            complement(12809..13159)
                     /locus_tag="VGM46_08270"
     CDS             complement(12809..13159)
                     /locus_tag="VGM46_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019856912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HEY4192629.1"
                     /translation="MTTTIYGIPNCDTMKKARAWLEGHGVTYAFHDYRADGLEADRLQ
                     GWIGKLGWEVLLNKASTTFRALADSDKQGLDAAKAKQLMLAHPTMIKRPVLELDGRLL
                     VGFKPAAYEDAFAK"
     gene            13370..13783
                     /locus_tag="VGM46_08275"
     CDS             13370..13783
                     /locus_tag="VGM46_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018241871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3597 domain-containing protein"
                     /protein_id="HEY4192630.1"
                     /translation="MGIFDKIKGAIFGRAEAAPAPTSAPAPSAGAANQPSAAPTPTAP
                     APSATTAQTVDVAAILEKAAAGKKQKLNWRTSIVDLMKALDMESSLAERKELAAELGY
                     TGSTSDSAAMNMWLHKALLKKLSENGGKVPADLLD"
BASE COUNT         2781 a         4381 c         4360 g         2549 t
ORIGIN      
        1 ggcttcgagg tccatatggg ctatgcgacg gcccgccacc gcgcggcaat cgatacgcac
       61 gggccggtgc ctcgcctgca ccgcatttcg ttcgcgccgt tccggctttc cggcgaggaa
      121 cctgtcgccg tggaatgaac cattcgaccg gcccggcagc caatagctac cccgcctgcc
      181 ccgctatcga gctatcttgc tatcttgcta ttttgctatt ttgctatttt gccggacata
      241 ccgcgactgt ccatccgtgg cacttgatga cgcccggata atcccctcgg tggacaggag
      301 aagcatagct gaaaataaaa aagccgctgg cttcccaggt ggctttttcc aacccatgat
      361 tggcaggctc agttgagctt ggacttcact tcctgaagcg aagccttgaa caggtccgcg
      421 ccggctttcg catcggcgtg gctggcgagc agcttgcgcg ccgcctctac cgccagatca
      481 acggcgctgg agcgaacctc ggcaatcgcg tcgcgctcgg cctggccgat cttctgttca
      541 gccaatgcgg tgcggcgcgc cacgtagtct tccgtgcgct gatgcgcctc ggaagcgaga
      601 atctcggcct cgcgcctggc cgcttctacg atggcgccgg cttcctgctc ggcttccttg
      661 cgcttcttct ggtattcggc cagcagcgcc tgtgcttctt cgcgcagggt gcgggcttct
      721 tccagttcgc tgcggatgcg cgcgccgcgc gtatcgagcg acttcgccat catgcccggc
      781 actttgatat aaatgcagat gccgatgaat atcagcagcg cgatcagcgc ccagagcgtt
      841 gcgagagagg ttgcgtccat tgcggtatcc tcagcttgcc acggatttca cggcggccga
      901 cacggctgcc ttgtcgcttt tgccgaccag ctgttcgagg atcgcgccgg ctgtactttc
      961 ggcaatggcg ccgacatcct tcatcgctgc ggtcttgatc gacgcgatgc ggccttcggc
     1021 ttcggtcaac ttcgcttcca ggccggcttc gaccttcttg cgagcggctt ccgcttcttt
     1081 cttggcatcg tcgcgcgcct gctggccgat ggcattggcg ttggccttcg cctcggccaa
     1141 tccctgttca taagcagcga cggcggcttc cgcctcgctc ttcagcttgt tggcctgatc
     1201 cagatcctgg aagatgcgcg aactgcgcgt atcgaagatg ccggcgaggc gcggcatcac
     1261 gatccttttc aggaagaggt aaaacaggcc gaaagtgatg accagccaaa agatttgcga
     1321 cgcgaacgtc gctggatcaa agggaggaaa cgtgccctca tgcgcggctt catggggaac
     1381 ttccgttccc gcatgggttt cgccggccgg ttgcgcatcc acgccggtga cggcgggtgc
     1441 tgtttcttgc ccataggctg gtgtcacgaa cataaattgt cctcgtcata cgggcccgac
     1501 cgcgtaaagc ggcgcggccc tgccttcagc ggttaatcag ccgaacagcg cgagcagggc
     1561 gatgagcagc gagaagatgc ccatggcttc cgtcacggca aagccgaaga tcaggcggcc
     1621 gaactggcca tcagctgccg acggattgcg cagagcgccc gccaggtagc tgccgaagat
     1681 gttaccgagg ccgataccgg ctccgcccat gcccaggcaa gcgatgccgg cgccgatgta
     1741 ctttgctgct tcaacgtcca ttttataact ccttgtgggt cccgatagtc tctttttcgc
     1801 cgacgcagcc gcgctgcgac cgaaccttag tgccctggat gcagggcgtc gttgaggtac
     1861 atgcaggtga ggatggcgaa cacgtaggcc tgaagcgccg ccaccagaat ttccagcgcc
     1921 gtgatggcaa ccgccatcgc cagcggcatg acggcgccgg cgatgccgac agcgcccatg
     1981 gcgctcagcg atacgacgaa gcccgcgaaa accttgagcg tgatgtggcc ggccagcatg
     2041 ttggcgaaca gacgaaccga aaggctgacc ggacgcgaaa tgaacgagat cacctcgatc
     2101 aggataacca gcggcatcag caccagcggc acgccgctcg gcacgaacag cttgaagaag
     2161 ccgaaaccgt gcttggcgat gccgtagacg acgacgatgc cgataaccag catggccagc
     2221 gtgaaggtga cgatgatctg gctggttacc gtgaagaagt aaggaaaaag gccaagcaga
     2281 ttggccgcca gcacgaacat gaagatggag aacacgaagg ggaagaactt catgccctgc
     2341 gttccggcgg catccttcag catcgaggcc gtgaactcat agagcatttc ggcgctcgac
     2401 tgccagcgcg acggcaccag cgcgcgcccg gaggtggcga aatagaagaa gctgccggcc
     2461 agaatgacga cgaccaccat gaacagcgac gaattggtaa aggacagatc ataaccaccg
     2521 agatcgatgg ggatgatctt gttgatatgg aactggtgga tcgggtcgac cttgtcagct
     2581 gccactttca atcctcacgc aagccgcgca cggcttttta aacccaacac gcaccttgcg
     2641 gcgcgcctca atcattcttc gggccggtgc tccggccgct ttgtccgaac ccggcgacct
     2701 tgcccaccga acgcagtatg ttcagtatgc cggcgcagaa cccgaggagc agaaacacga
     2761 tcaggcccca tggggaagta cccgcgaggc ggtcgatcat ccagccgatg ccgacaccca
     2821 ccactatccc ggcgatgaat tcgctggaaa gcttgagcgc ctggccatat ccagccaaac
     2881 tacccttttt cgcgttttct tcccccttga gttcttccgg cattcttgat gcaagcgatg
     2941 cttcaagctc acgccggcgg cgctcaagtt cgtcgtcgcg gatgtcgcct ttgctcaggt
     3001 gtccgtggcc ggtctctccg gtttcgcctg gccttttcct gtcggccatt cgcaaccccc
     3061 tgcccgccac aacgtcgccg tcggtccgca tccaagtcgc gggcaacata gttagagggc
     3121 cagcaaccgt caagccacgg cgccgactag aatggcaagt atttagccaa acgaaatcaa
     3181 cctcttagac aagtgcgaca tcgtgcccgc cgccaaagcc aggcctcaac ggcgcgaatc
     3241 ccgcccagcg gcgggcaatg gaaggctcaa ctccagccgc cgccataggt gcggtagaag
     3301 atgtgcaggc cgattttcgt catcttcttc atcgcccgcg cccagccggg gctgacatag
     3361 gcggcatagt agtgagtgga ggagccgacc tcgggaatga atatcttgcc ggcggtgacc
     3421 gccatggcga catccctggc gatcttgtag tgcgccgggc tttcgatgcg atccttgtgg
     3481 ccatcgcagg cgaacgaaaa ctggcaggcg ttgagccagt tctggttctg gtagacgacg
     3541 ccgcaaatgc tggccggata ggccgggttg cgcacgcggt tgagcactac ctgggcgacc
     3601 gccgcctggc cgcgcaatga ttcgctgcgc gcctcgaaat aaacggcgtt ggcgaggcat
     3661 gtctgttcct gtttggaaaa gacgctggcc ggcaatgggt tttgcagcca ggcatggtcg
     3721 cccttcgaca tgggcgggat gaagcgcccc tcgttcggct tgtcgtcctg caacagcgcc
     3781 tcgaagggag acgccttggc gtagtccggc tcggccggcg cataggcggt cgccagcacg
     3841 tcggcatggt cgttggtgac aagcgccgcc aacatggacg gaacgccggg tgcggccttc
     3901 ttgtcctccg tcgggtggaa ggcggcggct atctggattt ccttgccctt tatcgcgggc
     3961 ttggcaaatg tcagcttgag gccgctgttc atggtcggac gaagcagcga gctggtacgc
     4021 tggaagatcg aaccggcggt gaaattcttc ggcggggcca ccgggacgat ctggacgatg
     4081 cggcccttct tttgcgcccg cacgacgcga tcctcgtccg gcacggtgcc ggcggcgtcg
     4141 cccttgccac gaaatgatac cgagccgaca ccgggcagtt cgacgccggc gcccgaaatg
     4201 gagccggtcg tgagcgtatc gacaaacggc atttcagcgg catggacgga gccggccacg
     4261 gatttttcga cataggcatt ccagcgcgtc ggcgcctcaa gccccgaaat gaggctggtc
     4321 atgtcctgat aggcggtaac cgtcgggaag ccgatccaga tgccaagacc gactatcgcg
     4381 ggcgaaagga aggaacgctt cttgagatcg gcgctggccg cggcggcaac aagccgcttc
     4441 aaaacacgtc gatgcacggg actctccaga acgccactga ccccaaaata gaggttcaaa
     4501 gtgaagcatt aaccttgatg gagggttaat ttcagcggcg cagccctgcc ggtgcatggc
     4561 tgcaataggg ccgatcacgg attacaccag cctaccgcca aaggacgatg cgcccggcta
     4621 tttgttcaaa tggccggctg ggcgtgaaag gtcgatggtg cgggccgttg ccggagtaac
     4681 gcgtgcatgg acagtccatt cgatatcgtc gatgaaatgc ttggcatggc gaaagagccg
     4741 gccgagttca gcaaggacga cctggcagac atgatcctgg tcgcagccac ggaaatcatg
     4801 aagctcaggg aaagaatgca cccgccaagt ccggctgagc ggccggaacg cagaatgccg
     4861 cgtgcattca tgccgcagtt ttagccaaaa cccgaagaag cggtcaaaac cggcagatca
     4921 ggtgcgcttc tttgccctgc cggcaaacgg attgtcggag gtgcgcagcg ccatacggat
     4981 cggcacgccc ggtatgtcga acgcctggcg cagattgttg gtcaggtagc gcacatagga
     5041 ttgcggcatc gcgtccggcc gcgagcagga aacgacgaag cccggcgggc gcgtcttggc
     5101 ctgcgtcaga tatttgatct tcagccggcg gccggcgacg gcaggcggcg ggtgatgcgc
     5161 cagaatgcct tccagccaac ggttgagctt gccggtcgaa acgcgactgt tccatatccg
     5221 gtgcgtcttg atgacggctt ccatcagctt gtcgaggccg cggccggttt cggccgaaac
     5281 cggcacggcc tggataccgc gcacctgggg cagcaggcgc tcggtctttt cgcgcagttc
     5341 ggccagcatt tcctgcgggc gctcgatcat gtcccatttg ttgaaggcga tgaccggcgc
     5401 gcgtccctcg cggacaatca ggtcggcgat ctgcaaatcc tgcttctcga acggaattgt
     5461 cgcatcgagc acgatgatga cgatctcggc aaagcgaatg gcatgcaggc cgtcctgcac
     5521 cgacattttc tctagctttt cctggatgcg cgccttgcgg cgcatgcctg ccgtatcgaa
     5581 cagcttcatc ttgcggccgc gccaatccca atccacggaa atggaatcgc gggtaatgcc
     5641 ggcttccggg ccggtcagca ggcgttcttc gccgatcagc gcgttgatca tggtcgactt
     5701 gccggcattc ggccgcccga cgacggcgat gcgcatcggc ttggtatcgt cgtaggcggg
     5761 aacgtcctcg gcgtcgggat cagcaatgtc ctcgccgatc agcacttcgc tggcggcgat
     5821 ttcctcgtct ttttcctctt cctcgccaaa agccatcgcg gcgcccagcg cctcgaccac
     5881 ggcgtcgcgc aaatccggca ggccctggcc atgctcggcc gaaaccgcga ttggctcgcc
     5941 aaggcccagt tcccaggcct ccagcatgcc gccttgcgcg ccgcgcgcct cggccttgtt
     6001 ggcgacgagt acgaccggct tgccggactt gcgcacgatc tcggcaaagg tccggtcgtc
     6061 gggcaaaagg ccggccttgg catcgaccat gaagaagatc aggtccgcct cgcggatggc
     6121 gatttccgtc tgggcgcgca tgcggccctg aagcgtcgag gcggcggcat cttcgaagcc
     6181 ggcggtatcg atcacgtcga aatgcaggtc gtagagcttg gccgggtgga cgcggcggtc
     6241 gcgcgttacg cccggcgtgt catccaccaa tgccagcttc cggccggcaa gccggttgaa
     6301 aagcgtcgat ttaccgacat tgggccggcc gatgatcgca acctggaagc ccatcagctg
     6361 cctgaaccgc ggatcaggcc cgccatgatg gtggcgcgct ggcgcacatt ttgcggggcg
     6421 gcattgtcgg cgacgatctg gtcgaacagt ttttgcgcat cggctttctt gccgtccttc
     6481 caggctgcca ggccaagcgc ttcgcgcgcg ctgtggcgca gcgggttggt gtcgtcggtc
     6541 agcgcctcga cgcggcttga aacgtcgtca tatgagccgt ggtcgaccag cagcagggcc
     6601 gcccgcaacc gcgccagatc gcgcaggccg gcggggatgg cggtgtcggc ggcaacggcg
     6661 tcgaaatcct tgaccgcgcc ggcggcatcg cccttgtccg ccttgacggt tgcggcgcgc
     6721 atgcgggcaa gcagcggata cgcgccgtag ccatcctgtt caagcttggc gaaggcggcc
     6781 agcgcatcgt cgttcttgcc gctatcggcc aaggccagcg cctgcgaata ggcgtcgccc
     6841 gagcggttgg cgcgggtttc gttccagtag ctgtagccga cataggcggc cgtgccgata
     6901 atgatcagca gggcgatggc aagcagcgcc ggaccatagc ggtcccacat ggccttggcc
     6961 tgatccttgc ggatctcttc gttgacttcg cggataaagc tgtcgtcggc catggaaccg
     7021 tcattgtccg gctggcgcag gccttcgaaa accgaaaacg gctttgcaag gaagcctgcg
     7081 taaattcaag atgccgaagt ttgcctgccc gtccgatcga cgcgcggctc tccggccggc
     7141 tgattgagcc tcgcgtttta gcgaaatttt gccggcatgg aagggggagc ggccgaaatt
     7201 cggcgtaagc aggctaattg gaagcggcgg ggcgaaatcc acgaccgcac ccgcgccaca
     7261 ttttcccttt aaccggcagc ctgccctacc cggcaggttc cttttccagc agaccgtttt
     7321 cctatgccca gcctgtttcg cgtctcaaaa ctgttatgcg tccccacact gttattcgtc
     7381 tgggccttgc ttggcatcgc ggcgcccggc gcggcgcgcg cggacccgcc ttcgcccctc
     7441 gcgccggctg cgcccaagca aatcgtcatc atctccttcg acagcgcccg cgacatttca
     7501 caatggcagc gcagccgcgc cctggcgaaa cgcaccggcg cgcatttcac ctatttcctg
     7561 tcctgcgttt acctgctgtc gagggacacc cgcaaggaat acaccgcccc gcataaatcg
     7621 gcgggcaaat ccaatatcgg ctttgcgccg tccaaagaag atgtgactgc ccggctggag
     7681 caaatccggc tggcggcggc ggaagggcac gacatcgcca gccacgcctg cggccatttc
     7741 gacggcaagg actggagcaa ggccgactgg ctcaacgaat tcggctccgt ccgccggatt
     7801 ttcgagaacg cctatgccat caacggcatt gcgccggaac cgaccgactg gcgcgctttt
     7861 gcaaaaaccg ccgtggtcgg ctttcgcgcg ccctatcttt cgaccggagc cgcgctttat
     7921 caagctttgc aggcggcggg ttttgtctat gacgccagcg gcgtctcgcg cgggccggcc
     7981 acgcccgaaa ccgtcggcgc aatcacccgg ttttcgctgc cgctggtgcc ggaaggcccg
     8041 agaggcaagc cggtgatcgc tatggactat aacctttatg cccgccactc cggcgccaag
     8101 gaacagccgg cggacgcccg gcagttcgag gaacgcgcct atcaggcctt ccacacggca
     8161 ttcgaagcgc aatatgcggg caagcgcgtt ccattagaac ttggcttcca cttcacgctg
     8221 atgaatggcg gcgcctactg gaacgcgctg gaacgcttcg ccggtgaggt ttgcgtgaaa
     8281 gccgatgtcg aatgcatcag ctttcgcgat tatgtctcgc gcctgccgaa agccgcgccg
     8341 cgacaggacg gctagtgccg cctattcgac gggctgcgcc ctcgcctgtt cgcgctccag
     8401 atagcgctgg tcgatgtccg gcagcgcctc tcccctcagc gttgcctcga aagcgcgcag
     8461 gcgcttgtgc accgattgca actcgacgat cgtcgaccat gagctgagca ggaattgcag
     8521 ggactgcgtc accctgccga aggcgttcga tatctggttg agcgtgccga aggtgatctt
     8581 gtgggccacc agcgaagggc cgaggatgac gaaggagaag atgttgtccg cctgaaggta
     8641 ggtgtagcgc acgacgttga aatagacata gtggccgtaa aggcggaaat agttcttacg
     8701 cacattgtcg aacagctgcg cggtcgttac cggctgggcg cggtcctcgt gatcctcgcc
     8761 atagacgagt tccttgcggt aggcggcctc gacgcgctgg ttgcggaatt gcaggcccgg
     8821 cagcttgatg ccggcgacca tgacgagaac cgtgccgaac acgcaccagc caatcgccgc
     8881 gaccacgagc ggctgcggaa ccgcgccgat gatgggcagt tcggtgatgt gctgctgaag
     8941 gccgatcatg accggcagga aggcgatcaa cgtcatgatc gactggacga agctcgaccc
     9001 cagatcttcc atgatctggg aaaagcgcat cgtatcttcc tggatacgct gggaagcgcc
     9061 ttctatgtgg cgcagcgtac cccagttggc catgaaataa ttgttcatgg ccgtgcgcca
     9121 gcggaaaatc caatggctga cgatgaaggc gtttatcacc tgcacgttca tgccgacaag
     9181 cgccagccag gcgaagctgg cctgcccgcg atagaattcg ccggcggtcg attttgtcgt
     9241 tcccgacatc aggccctgga cgtaatcgaa gaagggaccg taccaggcat tgaccgcaac
     9301 gctgacctga accgtgaaat agatcaggaa aaggatgacg gcggtgacca gaatggacca
     9361 gcgctgccac ggatgcggcg aataccagct ccagaaagcg tagaacgccg ccaccagcaa
     9421 ggcgaaatag acataaaacc acaggaacgg cttcgaccag aacaccgcta tgccgacgat
     9481 gggcggcgcg cccgccgcgg caggcggcag gccgatcagc gcgccgagtt cggcgccgcc
     9541 gaaaaaccag aacaggatgg cggcaaggct ccacaagaca gccgaggtga agaacagctt
     9601 cggctgcgga aagaaggata cgaacacggc tttaatcccg acgggttgga gcgtttgcga
     9661 ttcgtcggca tcaagtcata gtttttgcgg caaagaaatg gtctgcacgc cgttggtgaa
     9721 ttacattctg gtcatgtaac cagagcgctt gtccgcttca gccggtcttg cgggccgacc
     9781 acgccagcgc gccggaagcg accagcaccg ccgacgccag gatcgacgag gcaaagaagc
     9841 tgcccgaccg ttcggcaagg gtaccggcga tgatcgggcc gatgatctgg ccgacgccga
     9901 aagccgccgt catcagggcg aacagccggc gcggcgattg cggagcgtgg cggcggccgg
     9961 cttgcaggcc aagcgcggtc gccgcgatga aggtgaggcc gagcagcaag cctgccagca
    10021 ccggccctat cctgccgccc agcgaaacgc tggccgcgac gccgacagcc tcgatgaggc
    10081 tggccagggc ataggcggca tagatgccgg tgcgcgcggc gatccagttc caaagccaga
    10141 ccgaaggcgc gccggcaagg ccggtgacga gccagaccat ggtttcaaaa acccgcccgc
    10201 cgccgccctg gcgcacgatg gtgatgagga aggtggcggt gatgacatag ccgaagccga
    10261 acaggccgta ggcgacaatg atcttcacca gcgaccggtc tttcggcagt ggcggctcgc
    10321 gttcggctgc gccattgccg ctgccggccg ggccttcgtc ggccatccat gcgacggcga
    10381 gaaacccaca ggcggcaatc accgccgacc acagccatcc ggctgtccag ccggcatggc
    10441 aaaggacaag cacggccagc agcccggccg acgccgcgat gccaagaccg acgccgccga
    10501 aatgcatggc ctggaggctt ccctgccgcc ccgccgtcgc atggctcaag acgatgctgg
    10561 tcaggaatac catgacgaaa gcgctggcga cgcccgcgaa gaaacggatg accagaaacg
    10621 ggatcatcgc ctgcgtcatt gccatcgatg cacaaaggat tgccgtggcg cccagcccgc
    10681 aaagggcggc aagcctctcc ctgccatgtg cccagccgcc cgccgccagc aaggccccgg
    10741 ccagatagcc gagataattc gccgaggcga tcaaaccggc gtcggaagcc gtcaggccca
    10801 gttcctccat catgccggga aggatcgggg tgtagacgaa gcggccgatg cccatggcga
    10861 cagccatgcc gatcatgccg gcgacgccaa atcttaactg agtgtcgaac agggttttca
    10921 tcgcggcgca ccatataagc gcactgcggc gagacaatcc gatttcgttg ggccaatggc
    10981 tgccggccag cggtgcgatg aggctggaaa ggtcggctgc ggcggtctat gtaacgggaa
    11041 accctatcaa ggcaggcgga accatgacca cccatatgcg cgatttttcg gcttcgatcg
    11101 acccggtgaa gctcgacagg ctggccgaag tggcgatcaa ggtcgggcta cagttgcagc
    11161 ccggtcagga tctggtgctg acctcgtcca tcgcggcgct gccgctgacc cgcaagattg
    11221 tcgagcatgc ctacaaggcc ggcgccggcc tggtgacacc gatcttcaac gacgacgaaa
    11281 tcacgctggc gcgttttcgc tatgggaacg atgccagctt cgaccacgcg gctggctggc
    11341 tctatgaggg catggccaag gcgttcggca acaatgcggc gcggctggcc gtgcgcggcg
    11401 acgacccgtc gctgctttcc agccaggacc cggcaaaagt atcgcgcgcc agcaaggcca
    11461 attcgatggc ttatcagccg gcgctggaga agatcaccgg tttcgacatc aactggaaca
    11521 tcgtcgccta tcccgagctt gcctgggcaa agcaggtatt tccaaacgaa acggacgatg
    11581 tggcggtggc taaactggcc gatgcgatct ttgcggcctc gcgcgtcgat gtcgacgacc
    11641 cgatcggcaa ctggaaggcg cacaatggcg ccctgcgcag ccgcaccgaa tggctgaacg
    11701 gccataactt ccactccctg cacttcaccg ggccgggaac cgacctgacc gtcgggctgg
    11761 ctgatggcca tgaatggatg ggcggagcct cgaccgccaa gaacggcatt acctgcaatc
    11821 ccaacattcc gaccgaggag gtgttcacca ccccgcatgc gcaccgtatt tccggccatg
    11881 tttcctcgac caagccgctg tcctaccagg gcacgctgat cgacaatatc tccgtgcgct
    11941 tcgaggaagg gcgcatcgtc gaggccagtg cgagccgggg cgaggacgtg ctgaacaagg
    12001 ttctggacac cgacgaaggc gcgcgccggc ttggcgaagt ggcgctggtg ccgcattcgt
    12061 cgccgatctc gaaaagcggc atcctgttct acaacacgct gttcgacgag aatgcgtcct
    12121 gtcacatcgc gctcggccag tgttattcga agtgctttgt cgacggcgca tcgctgacgc
    12181 cggacgagat tgcggcgcgc ggcggcaata agagcttcat ccacatcgac tggatgatcg
    12241 gctccgacaa gatcgacatc gatggcgtcc acaaggatgg ccgcagggtg ccggtgatgc
    12301 gcaagggtga atgggcctag cgtaggatga agatcgatct gacgatcaag cgcgtctatg
    12361 acccgcccgc gaaagcagat gggcagcggg tgcttgtcga ccggctgtgg ccgcgcggcc
    12421 tctccaagga caaggccgaa ctgacgcttt ggctgaagga aatcgcgccc agcaccgagt
    12481 tgcgcaaatg gttcgaccac gatccagcca aatggaccga attccagcgc cgttttcgcg
    12541 ccgaactgga cgagagcggc gaggcggtgg acgaattgcg cggcctcatc gccaagggca
    12601 aggtgacgct gctctacagc gcgcatgacg agacgcacaa caatgccgtg gcgctcgccg
    12661 cctatctgaa agaaaaagcc tagctggttt ttaccgaatc ggtaaaaccg ctctatctct
    12721 ttatctcttt gttttggctg catttccgga cgcaaaaccg tttcacattt ttgctggaaa
    12781 tgctctaggc gcgccctgcg ccggccgcct attttgcgaa tgcatcctca taggcggcag
    12841 gcttgaagcc gaccagcagg cggccgtcca attccagcac cgggcgcttg atcatggtcg
    12901 ggtgcgccag catcaactgc tttgccttgg ccgcgtcgag gccctgcttg tcgctgtcgg
    12961 ccagcgcgcg gaaagtggtg ctggccttgt tcaacagcac ctcccagcca agcttgccaa
    13021 tccacccctg aagccgatcc gcctcaagcc cgtcggcacg ataatcgtga aaggcatagg
    13081 tcacgccgtg accctccagc caggcgcgcg ccttcttcat ggtgtcgcaa ttggggatgc
    13141 cgtagatggt ggtggtcaag aaagcctcgc ctgtttctac ggtcagccta tgccggcggc
    13201 gggctgcgcg acaagtgccc cggagcgcca agcttaactt cttgttcgct ttttaggcag
    13261 cattgccctt gccgatagcg ccggcaggct agatagttcg ccgcgtgagt gaccgacgca
    13321 aaccgcgagc gcccggcaat ggccggcctc gacagatacg gagactaccg tgggaatctt
    13381 cgacaagatc aaaggcgcga ttttcggccg ggcggaagca gcaccggcgc caacttcagc
    13441 cccggcgcca tctgccggag ccgccaacca gccctccgcc gcgccaacgc cgacagctcc
    13501 cgcaccttct gccacaaccg cgcagaccgt ggacgtggcc gccattctcg aaaaggctgc
    13561 cgccggcaag aagcagaagc tgaactggcg cacctccatc gtcgacctga tgaaggcgct
    13621 ggacatggaa agcagccttg ccgaacgcaa ggaactggcc gccgagcttg gctacaccgg
    13681 ctcgaccagc gattcggccg cgatgaacat gtggctgcac aaggcgctgt tgaagaagct
    13741 gtctgaaaat ggcggcaagg tgccggcaga cctgctcgac tagagcattt ccagcaaaag
    13801 tgcgaaacgg ttttgcatcc ggaaatgctg ccaaaacaaa gagatagagt ggttttgtcg
    13861 attcggtaaa aatcgaaacc gctctagtat cgacgcaaca accggacctg ccagggagcg
    13921 gggcaaatgg cccgctgtta tgcccctgcc atccagatga attgcggcag cacgaaatcg
    13981 ggcgtcgcgc cgtgctgcgc gaacgctttt tccagttcgg cggggccaag ttcggcaagg
    14041 atgccggaac gcaccagcac cgacgagatg c
//
LOCUS       DASYCS010000255         8481 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2962907,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000255 DASYCS010000000
VERSION     DASYCS010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 8481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8481
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2962907"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..978)
                     /locus_tag="VGM46_08280"
     CDS             complement(<1..978)
                     /locus_tag="VGM46_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEY4192631.1"
                     /translation="MFFPYKTGRAALLASLAATLGTAAAQAQEAAPAAAPVLDTGNTA
                     WMLTSTALVLMMTIPGLALFYGGMVRKKNVLATVMQSFAICCLVTVLWFALGYSLAFT
                     DGGAANAWIGGLSKLFHRGITVSTLWPVGNIPEFVFSTFQMTFAIITPALIAGAFAER
                     MKFSAMLVFTALWLLIVYAPIAHWVWGGGFLGTAGVLDFAGGTVVHINAGVAGLVCAL
                     VMGKREGYGSTNMAPHNLVYSVIGAALLWVGWFGFNAGSELAADGLAGAAMLNTQVAT
                     AAAALAWMFAEWIIAGKPSVLGIISGAVAGLVAVTPAAGFVNPTGAFFIG"
     gene            complement(1034..1372)
                     /locus_tag="VGM46_08285"
     CDS             complement(1034..1372)
                     /locus_tag="VGM46_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEY4192632.1"
                     /translation="MKIVMAIIKPFKLDEVREALTALGIQGLTASEVKGYGRQKGHTE
                     IYRGAEYAVSFLPKIKIEVAVSADMADRVVEAITAAARTGQIGDGKVFVFGLDRAVRI
                     RTGETDSDAL"
     gene            complement(1592..2464)
                     /gene="tesB"
                     /locus_tag="VGM46_08290"
     CDS             complement(1592..2464)
                     /gene="tesB"
                     /locus_tag="VGM46_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006327119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HEY4192633.1"
                     /translation="MTTAMEHLLGILDLEKLEHNLYRGRSPQVNWQRVFGGQTIAQAL
                     VAAQGTVASDRHVHSLHGYFMRPGDIRVPILYEVDRIRDGTSFTTRRVVAIQHGQAIF
                     SLEASFQIDEEGLEHQVPMPLDVPPPEGLKNQRELLEMATHVPEAIRRFWAKERPLEL
                     KPVNTRHYESRDKLPPRQNVWIRLAGPIPDNRALHSVLLAYLSDMTLLDTSTFAHGRG
                     LFDPDIQAASLDHSMWFHRPHSLDDWLLYTQDSPSSSGSRGFSRGALYARDGTLIASV
                     AQEGLIRLRRQPPE"
     gene            2569..3858
                     /locus_tag="VGM46_08295"
     CDS             2569..3858
                     /locus_tag="VGM46_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006327118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis hydroxylase"
                     /protein_id="HEY4192634.1"
                     /translation="MAEREADRKKTALDVLVAGAGYVGLTAAVALKQARPSLRIAIVD
                     AAPAGAWQRDGRASAIAAAARRMLERLGAWEEIAPQAQAIMEMIVTDSRTSDPVRPVL
                     LSFAGEVGGGEVGDGEIGAGEPFAHMVANRDLNAALRRRAGELGVDLIEGVGVGGFDA
                     TGTGVTVHLADGATVAAKLLVAADGVNSKLRDMAGIKTVKWEYGQSGIVCTVGHERPH
                     QGRAEEHFLPAGPFATLPLAPGKDGRNRSSIVWVERTEDAERLVAGDELVFETELEQR
                     FGLKLGEISVESKPRAWPLGLTLARAFVAPRIALAGDAAHGIHPIAGQGLNLGFKDVA
                     ALAEVIVEADRLGQDFGALDVLERYQQWRRFDTVQMGVTTDVLNRLFSNDIAPLRAMR
                     DIGLGLVERMPRLKDFFIRQASGLSGGTPRLLKGEPI"
     gene            3958..5175
                     /locus_tag="VGM46_08300"
     CDS             3958..5175
                     /locus_tag="VGM46_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003498440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEY4192635.1"
                     /translation="MTLGVGGSTVEQELGKLRSMRDSVSPIGEEERAARLARAQAIMR
                     KEGIAALYLDASTSTFYFTGLRFHGSERMHGVIVPAEGEIVHISPTFEEAKLRSMLSG
                     PANVRCWQENDSPAELVTKVIGEMGYADGTVAIDPATPFFRFDALQSAGSGHRFINGG
                     VVTDACRRIKTVNEIALIKAAMGLTLQVQQATARILREGMTTIEVAEFVTEAHIRLGF
                     DAAPEFCIVLFGEPTAYPHGVPYPQTLKKGDMVLVDVGATLHGYYSDITRSYVFGEPS
                     ARQREIWGIAKRATQAAFDAAKAGSPCEAIDNAARGLIEAAGLGPGYATPGLPHRTGH
                     GLGLDVHEEPYIVQGNKTPLAAGMCFSNEPMIVVYGEFGVRLEDHIYMAEDGAHWFTE
                     PAHSIDDPFGQAK"
     gene            5193..5681
                     /locus_tag="VGM46_08305"
     CDS             5193..5681
                     /locus_tag="VGM46_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007754265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEY4192636.1"
                     /translation="MSLESVRAFFAAHAPDIEIIVTEASSATVQLAAEAHGVEPAQIA
                     KTICLRAGDAEMLIVTAGTSRLDNRKFRDRFAAKARMLEAGEVLALTSHPVGGVCPFG
                     LPAPMPVYCDVSLRQFAEVVPAAGATNAAVRIDPQRLASLTDAEWVDVCRDPEVAEAS
                     GA"
     gene            5813..6250
                     /locus_tag="VGM46_08310"
     CDS             5813..6250
                     /locus_tag="VGM46_08310"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008122197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY4192637.1"
                     /translation="MNRHATAIWNGSLTEGAGTLDTQSGAFNKQSYSFKGRFQDESGK
                     SGTNPEELIAAAHAGCFAMQLSHMLAENGTVATELNVEAVVTYGPAAGGGFEITESAL
                     KLTGKVPGIDAAKFKELAEGAKVECPVSKALGAIKVTLDAKLA"
     gene            complement(6513..7169)
                     /locus_tag="VGM46_08315"
     CDS             complement(6513..7169)
                     /locus_tag="VGM46_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018430303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HEY4192638.1"
                     /translation="MTDTDLPHLTPVEARILGCLLEKQLLTPDVYPLTLNALQSAANQ
                     KTSREPVMALEQVDINRTLKSLEEKGLARRMMASRADRYEQMASRKFSLTTPQSALLA
                     MLLLRGPQTLNELHTRTDRMANFGSADAVRTELDMLTSKHPPLAQEIGRGPGQREDRY
                     GHLLTGPIDIAALAAVHAPIATSASAPGTPAAAGMEARIATLEEQVAKLTARLDDLGG
                     "
     gene            complement(7188..7967)
                     /locus_tag="VGM46_08320"
     CDS             complement(7188..7967)
                     /locus_tag="VGM46_08320"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006467970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEY4192639.1"
                     /translation="MVEFALPKNSKMREGKTWPKPAGATNLREYRIYRWSPDDGENPS
                     IDTYFVDMDDCGPMVLDALLYIKNKIDPTLTLRRSCREGICGSCAMNIDGSNTLACTK
                     GSDEISGTIRIYPLPHMQVVKDLVPDLTNFYAQHASIEPWLHTVSPEPAKEWLQSHED
                     REKLDGLYECILCACCSTSCPSYWWNGDRYLGPATLLQAYRWLIDSRDEAKGERLDNL
                     EDPFRLYRCHTIMNCAQTCPKGLNPAKAIAEIKKMMVERRV"
     gene            complement(7978..>8481)
                     /locus_tag="VGM46_08325"
     CDS             complement(7978..>8481)
                     /locus_tag="VGM46_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008122202.1"
                     /note="part of four member fumarate reductase enzyme
                     complex FrdABCD which catalyzes the reduction of fumarate
                     to succinate during anaerobic respiration; FrdAB are the
                     catalytic subcomplex consisting of a flavoprotein subunit
                     and an iron-sulfur subunit, respectively; FrdCD are the
                     membrane components which interact with quinone and are
                     involved in electron transfer; the catalytic subunits are
                     similar to succinate dehydrogenase SdhAB; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     flavoprotein subunit"
                     /protein_id="HEY4192640.1"
                     /translation="NTASVDKIMDRFDRLRHANGSTPTATIRERMQHAMQEDAAVFRT
                     QESLENGCKRISEIWGELKDIKVFDRSMIWNSDLVETLELENLMANAITTVYGAEARK
                     ESRGAHAREDFESRDDANWRKHTLAHLSPEGEVSLSYRAVHTEPLLKEEDGGISLAKI
                     APKARVY"
BASE COUNT         1494 a         2570 c         2871 g         1546 t
ORIGIN      
        1 gccgatgaag aatgcgccgg tcgggttgac gaagccggcg gcgggcgtca ccgccacaag
       61 cccggcaacc gcgccggaga tgatgccgag cacgcttggc ttgccggcga tgatccattc
      121 ggcgaacatc caggcaagtg ctgccgctgc cgtcgcgacc tgcgtgttga gcatggccgc
      181 gcccgccagg ccatcggcgg caagctcgga gccggcgttg aagccgaacc agccgaccca
      241 cagaagggcc gcgccgatta ccgaatagac gaggttgtgc ggggccatgt tggtgctgcc
      301 atagccttcg cgcttgccca tcaccagcgc gcagaccagg ccggcgacac cggcgttgat
      361 gtggacgacc gtgccgccgg cgaagtcgag cacgccggcc gtaccgagga agccgccgcc
      421 ccagacccaa tgcgcgatcg gcgcatagac gatgagcagc cacagggcgg tgaacacgag
      481 catcgccgag aacttcatgc gttcggcgaa tgcgccggca atcagcgcgg gcgtgatgat
      541 ggcgaaggtc atctggaagg tcgagaagac gaattcagga atgttgccga cgggccaaag
      601 cgtcgagacg gtgatgccgc gatggaaaag cttggagagg ccgccgatcc aggcattggc
      661 cgcgccgcca tcggtgaagg cgagcgaata gccgagcgcg aaccacagca cggtgacgag
      721 gcagcagatg gcgaagctct gcatgacggt ggccagcacg ttcttcttgc gcaccatgcc
      781 gccgtaaaac agcgccagcc cgggaatggt catcatcagc accagcgcgg tcgatgtcag
      841 catccacgcc gtgttgccgg tgtcgagaac gggagcggcg gcgggcgccg cttcctgcgc
      901 ctgggcggca gcagtgccga gcgtggccgc cagcgaggcc agcagggcgg cgcgccccgt
      961 cttgtaggga aaaaacattc tcatcgatct ctccattgga attggttggc gcccggaccc
     1021 gtcggtcggg aatctagagc gcatcgctgt ccgtctcgcc ggtgcgaatg cgcaccgcgc
     1081 ggtcgagacc gaacacgaaa accttgccgt cgccgatctg gcccgtcctt gccgcagccg
     1141 tgatggcctc gaccacgcgg tcggccatgt cggcgctgac ggcgacctcg atcttgattt
     1201 tcggcaggaa ggaaacggca tattcagcgc cgcgatagat ttccgtatgc cccttttgcc
     1261 gcccgtagcc tttgacttcg ctggcggtca ggccctggat gcccagtgcc gtcaatgctt
     1321 cgcgcacctc atcgagcttg aacggcttga tgattgccat cacgattttc atccggttcg
     1381 ctccttgacc cggcgcggtt ttcgccgcgc gcccctgtcc tcaccggcgt aatgcggtgg
     1441 tgagtgacat caagggttca agttccgtgc cagtcttatc ggtacggaac caagcctttg
     1501 gaaaaattat atttttcata tcgacggcct gatgcgtccg gtgccggccg ggccacgaat
     1561 gcgcaaataa tgggcagtat gataatattg cctattctgg aggctgtcga cgcaggcgga
     1621 taaggccttc ctgcgccacc gaggcgatca gcgttccgtc gcgggcgtag agcgcgcccc
     1681 ggctgaaacc gcgcgagcct gaactcgagg ggctgtcttg cgtgtaaagc agccagtcgt
     1741 cgagcgaatg cgggcgatgg aaccacatcg aatgatcgag gctggctgcc tggatatcgg
     1801 ggtcgaacag gccgcgtccg tgggcgaagg tggaggtgtc gagcagcgtc atgtcggaga
     1861 ggtaggcgag caggaccgaa tgaagcgcgc gattgtcggg gatcggtccg gccagcctga
     1921 tccagacatt ttgccggggc ggcagcttgt cgcggctttc atagtggcgg gtattgaccg
     1981 gcttcagttc aagcggccgc tccttcgccc agaagcggcg gatcgcctcc ggcacatggg
     2041 tcgccatctc cagcagttct cgttggttct tgaggccttc gggcggcggt acgtccagcg
     2101 gcatcggaac ctggtgttcc agcccttcct catcgatctg gaacgaggct tccagcgaaa
     2161 aaatggcctg accatgctgg atcgccacca cgcggcgcgt ggtgaaggag gtgccgtcgc
     2221 ggatgcggtc gacctcatag aggatgggca ccctgatatc acccggccgc atgaaatagc
     2281 cgtgcagcga gtgaacgtgg cggtccgacg ccacagtgcc ctgggcggcg acaagcgcct
     2341 gcgcgatggt ctggccgccg aagacgcgct gccagttcac ctgtgggctg cgaccacgat
     2401 acagattgtg ttccagcttc tcgaggtcga gaatgccgag aagatgttcc atggccgtcg
     2461 tcatgatttc gcgcctttgc ggaaatagga tatggccggt ttccgacagg aacgaagggt
     2521 agtcaaggcg cgcgtcgcca aggggttccc gcgagaaaag gttcaggcat ggcagagcgc
     2581 gaagcggaca ggaaaaagac ggcgctcgac gtgctggtgg ccggcgccgg ctatgtcggg
     2641 ctgacggccg cagtcgcgct caaacaggcc cggccgagcc ttcgcatcgc cattgtcgat
     2701 gcggcacctg ccggcgcctg gcagcgggac ggacgcgcct ccgccatcgc tgcggcggcc
     2761 cggcgcatgc tggagcggct gggcgcgtgg gaagaaatcg cgccgcaggc acaggcgatc
     2821 atggaaatga tcgtcaccga ttcccgcact tccgatccgg tgcgcccggt gctgctgagt
     2881 tttgccggtg aggtaggcgg cggcgaagtg ggtgacggcg aaatcggcgc tggcgaacct
     2941 ttcgcccata tggtcgccaa ccgggacctg aacgccgcct tgcgccggcg cgccggcgaa
     3001 cttggcgtcg atctcatcga gggcgtcggc gtcggcgggt tcgacgccac cggcaccggc
     3061 gtcaccgtgc atctggcgga tggggcgacg gtcgcggcga aattgctggt cgctgccgat
     3121 ggcgtcaatt cgaagctgcg cgacatggcc ggcatcaaga cggtgaaatg ggaatatggc
     3181 cagtccggca tcgtctgcac ggtgggtcac gagcgtccgc atcagggccg cgcggaggag
     3241 cacttcctgc ctgccggccc cttcgccacg ctgccgctgg cgcccggcaa ggatggcagg
     3301 aaccgttcat cgatcgtctg ggtcgagcgc accgaagacg cggaaaggct ggttgccggc
     3361 gacgagctgg tgttcgaaac cgaacttgag cagcgcttcg ggctgaagct cggcgaaatc
     3421 agcgtcgaga gcaagccgcg cgcctggccg ctcggcctga cgctggcgcg cgccttcgtc
     3481 gcgccgcgca tcgcgctggc cggcgacgcg gcgcatggca ttcaccccat tgccgggcag
     3541 ggcctcaatc tcggtttcaa ggatgtggcg gcattggcgg aggtgatcgt cgaggccgat
     3601 cggctcggcc aggatttcgg cgcgctcgac gtgctggagc gctatcagca gtggcgccgg
     3661 ttcgacacgg tgcagatggg cgttaccacc gatgtgctca accggctgtt ttccaacgac
     3721 atcgcgccgc tgcgcgccat gcgcgacatc ggccttggcc tggtcgagcg gatgccgcgc
     3781 ctcaaggatt tcttcatccg ccaggcgtcg ggactttctg gcggcacgcc gcgcctgctg
     3841 aagggcgagc cgatctgacc cgtatcgatt ccgctgtgga tcattgacgc gcgccaccgt
     3901 gttggcgatt ggcttgaccc taattgacct cccctcatgc aaattgaagg cttgaagatg
     3961 accctaggcg ttggcggctc cacggttgag caggaactgg gcaagttgcg ctcgatgcgc
     4021 gacagcgttt cgcccatcgg cgaagaggaa cgcgccgcgc ggctcgccag ggcgcaggcg
     4081 atcatgcgca aggaaggcat cgccgcactt tatctcgatg cctcgaccag cacgttctat
     4141 ttcaccgggc tgcgcttcca tgggtcagag cgcatgcatg gcgtgatcgt tccggccgag
     4201 ggcgaaatcg ttcatatcag cccgactttc gaggaagcca agctgcgctc gatgctgagc
     4261 gggccggcca atgtgcgctg ctggcaggaa aatgacagcc cggcggagct ggtgacgaag
     4321 gtcatcggcg aaatgggcta tgcggatggc accgtcgcca tcgacccggc gacgccgttc
     4381 ttccggttcg atgccttgca gagcgcgggc agcggccatc gcttcatcaa tggcggcgtg
     4441 gtgaccgatg cctgccgccg catcaagacg gtaaacgaga ttgcgctcat caaggccgcc
     4501 atggggctga cgctacaggt acagcaggcg acggcgcgta tcctgcgcga gggcatgacg
     4561 accatcgaag tggccgagtt cgtcactgag gcgcatatcc ggctgggctt cgatgccgcg
     4621 ccggaattct gcatcgtttt gttcggcgag ccgacggcct atccgcatgg cgtaccctat
     4681 ccgcagacgc tgaaaaaagg cgacatggtg ctggtcgatg tcggcgcgac gctgcatggc
     4741 tattactccg acattacgcg gagctatgtg ttcggcgagc cgagcgcgcg ccagcgcgaa
     4801 atatggggca tcgccaagcg ggccacgcag gccgccttcg atgccgcgaa ggccggttcg
     4861 ccatgcgagg cgatcgacaa cgcggcgcgt ggcctgatcg aggccgcagg cctcgggccg
     4921 ggctatgcga cacccggcct gccgcatcgc acgggccacg gccttggcct cgacgtgcat
     4981 gaggagcctt atatcgtgca gggcaacaag acgccgctcg ccgcaggcat gtgcttttcc
     5041 aacgagccga tgatcgtcgt ctatggcgag ttcggcgtgc gcctggaaga ccatatctat
     5101 atggccgaag acggcgcgca ctggttcacc gagccggcgc actcgatcga cgatccgttc
     5161 ggccaggcaa aatagaacgc taaggttgcc tcatgtcgct tgaatccgtc cgggctttct
     5221 tcgccgccca tgcccccgat atcgagatca tcgttaccga agcgagttcg gcaacggtgc
     5281 agttggcagc cgaagcgcat ggcgtcgagc cggcgcagat cgccaagacc atctgcctgc
     5341 gcgccggcga cgccgaaatg ctgatcgtca cggccggcac gtcgcgcctc gacaaccgca
     5401 agttccgcga ccgcttcgcc gccaaggcgc gcatgctgga ggccggcgag gtgctggcgc
     5461 tgaccagtca tcccgtcggc ggcgtctgcc cgttcggcct gccggcgccg atgccggtct
     5521 attgcgacgt gtcgttgcga caatttgcgg aagtggtgcc ggctgccggc gccaccaacg
     5581 cggcggtacg gatcgacccg cagcggctgg catcgctgac cgacgccgaa tgggtggacg
     5641 tctgccgcga tccggaggtt gcggaagcgt cgggagcata gaggtttttc ggcgcggctg
     5701 ttccgggcgg aataccgcgc ttgttagtgt ggcgcgataa gcaatactat gtctgtcggg
     5761 aacaacgcat gattgcggtc tgcccggcct tttgcaaccc aaggagaaag ccatgaaccg
     5821 tcacgccacc gccatctgga atggctcgct caccgagggc gcaggcacgc tcgacacgca
     5881 gagcggggcc ttcaacaagc agagctattc gttcaaaggg cgcttccagg acgaaagcgg
     5941 caagtccggc accaacccgg aggaattgat tgcggcggcg catgccggct gctttgccat
     6001 gcagctttcg catatgctgg ccgaaaatgg cacggtagcg accgaactga atgtcgaggc
     6061 ggtcgtcacc tacggcccgg cggcgggcgg cggcttcgaa atcaccgaaa gcgcgctcaa
     6121 gctaaccggc aaggtgccgg gcatcgacgc ggcgaagttc aaggaactgg ccgaaggcgc
     6181 gaaggtcgaa tgcccggttt ccaaggcgct cggcgccatc aaggtgacgc tcgacgccaa
     6241 gctggcgtaa ggcggcgaat gatcgacgtc gccggagcat ttccagcaaa agtgtgaaat
     6301 ggttttgcgt ccggaaatgc ggccaaaaca aagagatgga gcggttttac cgatccgtta
     6361 aagccagaac cgctctagct gcgggcttgc cccgaggatc cgcgccgccg gcaatatcgg
     6421 gtcgagaaat acgccggcta accatccggc atgaatggaa cttcgagaca ggccggggat
     6481 ggcggcgcgg cttggctcgc gctgtctatg cttcaaccgc caaggtcgtc gagccgcgca
     6541 gtcagtttcg ccacctgttc ttcgagggtg gcgatgcgtg cttccattcc tgcggcggct
     6601 ggtgtgccgg gagcggatgc cgacgtagca atcggagcgt gcacggcggc cagggcggca
     6661 atgtcgatgg ggccggtcag caggtgcccg tagcggtcct cgcgctggcc ggggccacgg
     6721 ccgatctcct gcgccagcgg cgggtgcttg ctggtcagca tgtccagttc ggtgcgcacg
     6781 gcatcggcgg acccgaaatt ggccatgcgg tcggtgcggg tgtgcaattc gttgagcgtc
     6841 tgcggcccgc gcaaaagcag catggcgagc agggcgcttt gcggcgtggt caggctgaat
     6901 tttcgcgatg ccatctgctc gtatcggtcg gcgcgcgaag ccatcatccg ccgggcaagg
     6961 cctttttctt cgagggattt cagggtgcgg ttgatgtcga cctgctccag cgccatgacc
     7021 ggctcgcgcg acgtcttctg gttggcggcg gattgcagcg cattgagcgt cagcggatag
     7081 acatccggcg tcagcagctg cttttccaga aggcagccga ggatgcgggc ttcgacgggc
     7141 gtcaggtggg ggaggtcggt gtcagtcatg gcccgccccg tatccagcta tacccttctc
     7201 tccaccatca tcttcttgat ctccgcgatc gccttggccg ggttcaggcc tttggggcag
     7261 gtctgggcgc agttcatgat ggtgtggcag cggtagaggc ggaaggggtc ttcgaggttg
     7321 tcgagccgct cgcccttggc ttcgtcgcgg ctatcaatca gccagcgata ggcttgcaaa
     7381 agcgttgccg ggccgagata gcggtcgccg ttccaccaat agctcgggca ggaggtcgag
     7441 cagcaggcgc acaggatgca ctcgtacagg ccgtccagtt tttcgcggtc ttcgtggctt
     7501 tgcagccatt ccttggccgg ttcgggcgac accgtgtgca gccatggctc gatcgaggcg
     7561 tgctgggcgt aaaaattggt gaggtcgggc accagatcct tgaccacctg catgtgcggc
     7621 agcggataaa tccggatcgt gccggaaatc tcgtcgctgc ccttggtgca ggcaagcgtg
     7681 ttcgaaccgt cgatgttcat ggcgcaggag ccgcaaatgc cttcgcggca ggaacggcgc
     7741 agcgtcagcg tcgggtcgat cttgttcttg atgtagagca gcgcatcgag aaccatcggc
     7801 ccgcaatcgt ccatgtcgac gaaataggtg tcgatgctcg ggttctcgcc gtcatcgggc
     7861 gaccagcggt agatgcgata ttcgcgcaga ttggtggcgc ccgccggctt cggccaggtc
     7921 ttgccttcgc gcatcttgga attcttaggc agtgcgaatt cgaccatgat gcgatcctca
     7981 gtaaacccgc gccttgggcg cgatcttggc gaggctgatg ccgccgtctt cttccttcag
     8041 cagcggctcg gtgtggaccg cgcggtagct gagcgaaacc tcgccttccg gcgacaggtg
     8101 ggcaagcgta tgcttgcgcc agttggcgtc gtcgcggctc tcgaagtcct cgcgggcgtg
     8161 ggcgccgcgg ctttccttgc gcgcttcggc gccatagacg gtggtgatgg cgttggccat
     8221 caggttttcc agttccagcg tctcgaccag atcggaattc cagatcatcg agcggtcgaa
     8281 caccttgatg tccttcagct cgccccaaat ctccgagatg cgcttgcagc cgttttccag
     8341 cgattcctgc gtgcggaaca cggcggcgtc ttcctgcatg gcatgctgca tccgctcgcg
     8401 gatggtggcg gtcggggtcg agccgttggc gtggcgcagg cggtcgaagc gatccatgat
     8461 cttgtcgacc gaagcggtgt t
//
LOCUS       DASYCS010000256         5569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4269043,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000256 DASYCS010000000
VERSION     DASYCS010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 5569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5569
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4269043"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1265
                     /gene="recG"
                     /locus_tag="VGM46_08330"
     CDS             <1..1265
                     /gene="recG"
                     /locus_tag="VGM46_08330"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006073713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEY4192641.1"
                     /translation="LAEINSDLGEPDRMLRLLQGDVGSGKTVVALLAMTRAVEAGGQA
                     VLMAPTEILARQHLATIAPLAERAGLRVAILTGREKGRDRADTLAGLASGDIDIAIGT
                     HALFQEAVSFKNLVFAVIDEQHRFGVHQRLAIDAKGDAPDMLVMTATPIPRTLVLTAF
                     GDMDVSKLTEKPIGRQPIRTVTLPLERLDELVGRMREAVEGGQKIYWICPLVEESEEI
                     KLMSAQDRFASLKPLFGNRIGLVHGRMKGAEKDEAMRAFKEGETRILIATTVIEVGVD
                     VPDATIMVIEHAERFGLAQLHQLRGRVGRGSKPSSCVLLYKEPLGETAKRRLSVMRET
                     EDGFLIAEEDLKLRGEGELLGTRQSGAPGFQVARIEAHADLLEAARDDARLILSRDPE
                     LQSERGEAVRLLLYLFGRDEAVRLLRAG"
     gene            complement(1217..1966)
                     /locus_tag="VGM46_08335"
     CDS             complement(1217..1966)
                     /locus_tag="VGM46_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015317641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEY4192642.1"
                     /translation="MSDLPKTSAMTRLRNYFLTGFIVCAPLAITAYIAWSFIGWVDSW
                     VKPYIPARYSPETYLPFRIPGFGLIVALVLITLIGFLTANIVGRAIVNFGERLLGRMP
                     LVRGIYRSLKQIFETVLSNKGDMFSQVGLVEYPRKGVWSLVFIGGEKDTEINQKLLVE
                     GDPLMGVFMPCTPNPTTGFLMYVRKSEVVMLDMTIEEGARLIVSAGLVAPPVKKKLPG
                     PDGELIEIANPTLGEVQPARSKRTASSRPNR"
     gene            complement(2028..2330)
                     /locus_tag="VGM46_08340"
     CDS             complement(2028..2330)
                     /locus_tag="VGM46_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HEY4192643.1"
                     /translation="MALKFARPIHPGEFLREEYLLPLGLSAGALAGKLNLPRTRIERI
                     AKEEIGMTPDTALRLARYFNTTAEFWMNFQQAYELETEARKLATELDKIEPLDAAA"
     gene            complement(2348..2632)
                     /locus_tag="VGM46_08345"
     CDS             complement(2348..2632)
                     /locus_tag="VGM46_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEY4192644.1"
                     /translation="MPIISFKGKAVEAVAAGKTPKGFPADMVSRAERGLRAVASAVEL
                     GDLRSPPGNHLEALKGDRVGQHSIRINDQWLICFRWTPSGAEDVEIVDYH"
     gene            complement(2724..4547)
                     /gene="glmS"
                     /locus_tag="VGM46_08350"
     CDS             complement(2724..4547)
                     /gene="glmS"
                     /locus_tag="VGM46_08350"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010967462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEY4192645.1"
                     /translation="MCGIVAIVGRNPVAPLIVDALKRLEYRGYDSAGVATIEAGELGR
                     RRAEGKLVNLERRLQAEPLGGNIGIGHTRWATHGVPNETNAHPHFSSGVAVVHNGIIE
                     NFADLRDELIRDGYAFSSQTDTEVVAHLVSRELKRGAAPVEAAHKAIKLLNGAFALAI
                     LFEGDDNLVVGARNGPPLAVGHGDGEMFLGSDAIALAPFTNSITYLEDGDWAVVRRDE
                     IAIFDIDGNKVERKRQQSLGTSYMVDKGNHRHFMEKEIHEQPEVIAHTLAHYLDFASG
                     TSRPLDLPFDFAKIDRLAISACGTAYLSGLVGKYWFERLARLPVDIDIASEFRYREMP
                     LSKDSAALFISQSGETADTLASLRYCRKSGLPIGAVVNVRESTIARESDVTLPTLAGP
                     EIGVASTKAFTCQLSVLAALAVRAAKARGKITPDEEKTLVRQLSEAPRFATQVLKLDT
                     QIEKLARELAHYKHVLYLGRDTNFPLAMEGALKLKEISYIHAEGYAAGELKHGPIALI
                     DENMPVIVIAPHDRIFEKTVSNMQEVAARGGKIILITDQKGAAQATVDTLQTIVLPDV
                     PEIISPNIYALPIQMLAYYTAVFMGTDVDQPRNLAKSVTVE"
     gene            complement(4709..>5569)
                     /locus_tag="VGM46_08355"
     CDS             complement(4709..>5569)
                     /locus_tag="VGM46_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HEY4192646.1"
                     /translation="DEERKIKFCNGGLMAIAGSHALKLLDAVGNQNAKGEYYLTDIVE
                     IAAAQGLEVVATEASFENALGINNRAELAEAEGIWQKRRRREAMLSGVTLVAPETVFF
                     SHDTEIGADTVVEPNVWFGPGVAIADGAKIHAFSHIEGAMIGPGADVGPFARLRPGAD
                     LKARAKVGNFCEVKKATIEEGAKVNHLTYIGDARIGAGANIGAGTITCNYDGYSKFIT
                     DIGENAFIGSNSSLVAPVSIGNGAYVASGSVITESVPDDALAFGRARQKTLPGKAKEL
                     RERRASAAGK"
BASE COUNT         1024 a         1702 c         1787 g         1056 t
ORIGIN      
        1 cgctggccga gatcaacagc gatcttggcg agcccgaccg catgctgcgc ctgctgcaag
       61 gcgacgtcgg ttcgggcaag acggtggtgg cgctgctggc catgacgcgc gcggtggagg
      121 ccggcggcca ggccgtgctg atggcaccga ccgaaatcct ggcgcgccag catctggcga
      181 cgatcgcgcc tttggccgag cgcgcgggcc ttcgcgtcgc cattttgact gggcgcgaaa
      241 aaggacgcga ccgcgccgac acccttgccg gccttgccag tggcgatatc gacatcgcca
      301 tcggcacgca tgcgctgttc caggaagcgg tatcgttcaa gaacctcgtt ttcgccgtca
      361 ttgacgaaca gcatcgcttc ggcgtgcatc agcgacttgc catcgacgcc aagggcgacg
      421 cgcccgacat gctggtgatg acggcgacgc cgatcccgcg cacgctggtg ctgacggcgt
      481 tcggcgacat ggacgtgtcg aaactgaccg aaaaacccat cggccgccag ccgatccgta
      541 ccgtcaccct gccgctggag cgtctggacg aactggtggg gcgcatgcgc gaggcggtgg
      601 aaggcggcca gaagatttac tggatttgtc cgttggtcga agaatccgaa gagatcaagc
      661 tgatgtcggc gcaggaccgc ttcgcatcgc tcaaaccgct gttcggcaac cgcatcggcc
      721 ttgtccatgg ccgcatgaag ggcgcggaga aggacgaggc gatgcgcgcc ttcaaggaag
      781 gcgaaacgcg catcctgatc gccacgaccg tcatcgaggt cggcgtcgac gtgccggacg
      841 ccaccatcat ggtgatcgag cacgccgagc gcttcggcct ggcgcaactg caccagttgc
      901 gcgggcgggt cgggcgcggc tcgaagccgt cgtcctgcgt gctgctctac aaggagccgc
      961 tgggtgaaac ggcgaagcgg cggctttcgg tgatgcgaga gaccgaggac ggcttcctca
     1021 ttgccgagga agatttgaaa ctgcgcggcg aaggcgagtt gctgggcact cgccagtccg
     1081 gcgcgccggg cttccaggtg gcgcgcatcg aggcgcatgc cgacctgctg gaggctgccc
     1141 gcgacgatgc caggctgatc ctttcgcgcg acccggaact gcaatcggag cgcggcgaag
     1201 cggttcggct gttgctttat ctgttcggcc gcgacgaggc ggttcgcttg ctgcgggctg
     1261 gctgaacctc gcccagcgtc ggattggcga tttcaatcag ctcgccatcc gggccgggca
     1321 gtttcttctt aaccggaggg gcgacgaggc cggcggagac gatcaaccgg gcgccctcct
     1381 ctatcgtcat atcgagcatg acgacttccg acttgcgcac atacatcagg aaaccggtgg
     1441 tcggattggg cgtgcacggc atgaagacgc ccatcagcgg atcgccctcg acgagcagtt
     1501 tctggttgat ctcggtgtct ttttcgccgc cgatgaagac cagcgaccag acgcccttgc
     1561 gcggatattc gaccaggccg acctggctga acatgtcgcc cttgttggaa agaaccgttt
     1621 cgaagatctg cttcagcgaa cgatagatgc cgcgcaccag cggcatgcgg ccaagcaggc
     1681 gctcgccgaa attgacaatg gcgcggccaa cgatattggc ggtgaggaag ccgatcaacg
     1741 tgatgagcac gagcgcaacg atgaggccga agcctggaat gcggaacggc agataggttt
     1801 cggggctgta gcgggcgggg atatagggct tcacccacga atcgacccag ccaatgaacg
     1861 accaagcgat ataggcggtg atcgccagcg gcgcgcagac gatgaagccg gtcaggaaat
     1921 agttcctcaa ccgcgtcatg gcggaagtct tgggcaggtc ggacatcgtt caccgtttcg
     1981 ttgcggtgca acgttaggcg agcggcaggc gcattggaac cggcaatcta cgccgcggcg
     2041 tccagcggct cgatcttatc cagttccgta gccagtttcc ttgcctcggt ctcaagttca
     2101 taagcctgct ggaagttcat ccagaattcg gcggtcgtgt tgaagtagcg cgcgagacga
     2161 agcgccgtgt ccggcgtcat gccgatttcc tccttggcga tccgctcgat gcgggtgcgc
     2221 ggcaggttga gttttccggc aagcgcgccc gcgctcaagc caagcggaag caggtattcc
     2281 tcacgcagga attcaccagg atggatgggg cgcgcaaact tcaatgccat ttctatcctc
     2341 catgcagcta gtggtaatcg acgatctcga catcttcggc acctgatggc gtccaccgaa
     2401 agcagataag ccattgatcg ttgatccgga tcgaatgctg gccgacgcga tcacctttca
     2461 atgcctcaag atgattgccg ggcggggagc gaagatcgcc aagctcaacc gcactggcaa
     2521 cggcccgcaa cccgcgctcg gcacgtgaaa ccatatccgc aggaaaacct ttcggcgtct
     2581 tgccagctgc aacggcttcc accgccttgc ctttgaacga aataatcggc acgccgcctc
     2641 cgtgctccgt atcatacagt gatacggcct ggaaatcaag gcatatgtat caatgaatga
     2701 tacaggcgag cggagatgcc gccctattcc accgtcaccg acttcgccaa attgcgcggc
     2761 tggtcgacat cggtgcccat gaagacggcg gtgtaatagg ccagcatctg gatcggcagg
     2821 gcgtagatgt tgggcgagat gatttccggc acatccggca gcacgatcgt ttgcagcgtg
     2881 tcgacggtcg cctgggccgc gcccttctgg tcggtgatga ggatgatctt gccgccgcgc
     2941 gccgcgactt cctgcatgtt cgagacggtc ttctcaaaga tgcggtcgtg cggggcgatt
     3001 acgatgaccg gcatgttctc gtcgatcagc gcgatcggcc catgcttgag ttcgcccgcc
     3061 gcatagccct cggcatggat gtaggagatt tccttgagct tcagcgcgcc ttccatggcg
     3121 agcgggaaat tggtgtcgcg gccaaggtaa agcacatgct tgtagtgggc cagttcgcgc
     3181 gccagtttct cgatctgggt gtccagcttc agcacctggg tggcgaagcg cggcgcttcc
     3241 gacaactgac gcaccaacgt tttttcttcg tcgggcgtga tcttgccgcg cgccttggcg
     3301 gcgcgcacgg caagggcggc cagcacggaa agctggcagg tgaaggcctt ggtcgaggca
     3361 acgccgattt cggggccggc gagcgtcggc agggtcacgt cgctctcgcg cgcaatggtc
     3421 gattcgcgca cgttcaccac cgcgcctatc ggcaagcccg acttgcggca atagcgcagc
     3481 gaggccagcg tgtcggcggt ttcgcccgac tgcgagatga acagggcggc gctatccttc
     3541 gacagcggca tttcgcggta gcggaattcg gacgctatat cgatgtcgac cggcaggcga
     3601 gccaggcgct cgaaccagta tttgccgaca aggccgctga gataggcggt gccgcaggcc
     3661 gagatcgcca ggcggtcgat cttggcgaag tcgaaaggca ggtccagcgg ccgcgatgtg
     3721 ccgctggcga aatcgaggta atgcgccagc gtatgcgcga tcacttcggg ctgctcatgg
     3781 atttcctttt ccatgaaatg gcggtggttg cccttgtcga ccatatagct ggtgcctagc
     3841 gactgctggc gcttgcgctc caccttgttg ccgtcgatgt cgaaaatggc gatctcgtcg
     3901 cggcgcacca ccgcccagtc gccgtcttcc agataggtga tcgagttggt gaaaggggcg
     3961 agcgcaatcg cgtcggaacc caggaacatc tcgccatcgc cgtggccgac agccagcggc
     4021 gggccgttgc gggcgccgac gacgaggtta tcgtcgccct cgaacagaat ggccagcgcg
     4081 aaagcgccat tcagaagctt gatcgcctta tgcgcagcct cgaccggcgc tgcgccacgc
     4141 ttgagttcac gcgaaaccag atgggcgacc acttcggtgt cggtctgcga agagaaggcg
     4201 taaccgtcgc ggatcagttc gtcgcgcaga tcggcgaaat tctcgatgat gccattatgg
     4261 acgacggcga cgccgctgga aaaatgcgga tgcgcattgg tttcgttcgg cacgccatgc
     4321 gtcgcccaac gggtgtggcc gatgccgata ttgccgccaa gcggctcggc ctggaggcgg
     4381 cgttcgaggt tgaccagctt gccctcggcg cggcggcggc caagctcgcc ggcctcgatg
     4441 gtggcgacgc cggcggaatc gtagccgcga tattcaagcc gcttcagcgc atcgacgata
     4501 agcggggcaa ccgggttccg gccgacgatt gcgacgatgc cacacatgct tgctttcccc
     4561 gtccacgatt cgcgaagtcc tgcggctctc tagagaatgc cggcctgtcg cggaaatcac
     4621 attgcgtttc gccgcatatc ggcaaaacct aattctcgac cgtccttagg acaggccacg
     4681 cctgaaatcc agttctccga accctgcttt actttcccgc tgccgaggcg cggcgttcgc
     4741 gcagttcctt cgccttgccc ggcaacgtct tctggcgggc gcgaccgaag gcgagcgcgt
     4801 cgtccggcac gctttcggtg atgacgctgc ccgacgcgac ataggcgcca ttgccgatgg
     4861 aaacgggtgc taccagcgag gaattggagc cgatgaaggc gttttcgcca atgtcggtga
     4921 tgaatttcga gtagccgtca taattgcagg tgatggtgcc cgcgccgata ttggcgcccg
     4981 cgccgatgcg cgcgtcgccg atataggtca ggtggttgac cttggcgcct tcctcgatgg
     5041 tcgccttctt gacctcgcag aaattgccga ccttggccct tgccttcagg tcagcgcccg
     5101 ggcgcagccg cgcaaacggt ccgacatcgg cgcccggccc gatcattgcc ccttcaatat
     5161 ggctgaaggc atggattttc gccccatcgg cgatggcgac gcccggaccg aaccagacat
     5221 tcggctcgac gaccgtgtcg gcgccgatct cggtgtcgtg cgagaaaaac acggtttccg
     5281 gcgcgaccag cgtgacgccg gaaagcatcg cctcgcggcg gcggcgcttt tgccagatgc
     5341 cttcggcctc cgccagttcg gcccggttgt tgatgccgag cgcgttttcg aaactggcct
     5401 cggtagcaac cacctccagc ccttgcgcgg cggcgatctc gacgatgtcg gtcaggtaat
     5461 attcgccctt ggcgttctgg ttgccgaccg catcgagcag tttcagcgca tggctgccgg
     5521 caatcgccat caggccgcca ttgcagaact ttatcttccg ctcctcgtc
//
LOCUS       DASYCS010000257         5003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3544731,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000257 DASYCS010000000
VERSION     DASYCS010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 5003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5003
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3544731"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VGM46_08360"
     CDS             <1..309
                     /locus_tag="VGM46_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862280.1"
                     /note="catalyzes the oxygen-independent formation of
                     protoporphyrinogen-IX from coproporphyrinogen-III; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HEY4192647.1"
                     /translation="VTIAERMPETWASLVEAKGHGVVDGEVLSRGEEADEFLLMGLRL
                     VEGIDLARYEQFSGRGLSSTRLSTLQDEGLVSPIGNSRLRATPAGMIVLDAVVADLAR
                     "
     gene            592..1503
                     /gene="rsmI"
                     /locus_tag="VGM46_08365"
     CDS             592..1503
                     /gene="rsmI"
                     /locus_tag="VGM46_08365"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEY4192648.1"
                     /translation="MPAETTPHRYMIGQTEMVARPLEAALYLVATPIGNLGDITLRAL
                     ETLAAADVLACEDTRVSRVLLARYGIRRRTTAYHEHNSAEAGPKLIAALEAGQSVALI
                     SDAGTPLISDPGYRLVGEALEKNIRVVPIPGASAPLAALTASGLPSDAFLFAGFLPVK
                     TGQKLARLQELKIVPATLIFFESPRRLADTLAAMVEALGGERRATIGRELTKTFEEMR
                     PGALATLAEHYAAADTPKGEIVICVGPPDHAVEEPADIDRLLLSLAKEMPASKAAAEA
                     AKMTGGQKQALYRRLLELRDAAEDDGD"
     gene            1478..1873
                     /locus_tag="VGM46_08370"
     CDS             1478..1873
                     /locus_tag="VGM46_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEY4192649.1"
                     /translation="MPQRTTVTESATAKRRKSHQRGHRGEGLAAMALRLKGFRILARR
                     YRTKLGEIDLIARRGDLVLIVEVKARRTLIEAMQAIAFESERRIEGAADLWLSRQPDY
                     GRLSVRFDMVAVLPWRWPVHVENAFHGRN"
     gene            1976..2206
                     /locus_tag="VGM46_08375"
     CDS             1976..2206
                     /locus_tag="VGM46_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3297 family protein"
                     /protein_id="HEY4192650.1"
                     /translation="MTLPDRMSVDERSPFYNAEALERGVGILFNGKEREDVSEYCVSE
                     GWIRVATPKSKDRFGNPLTIKLKGEVKPFVKE"
     gene            complement(2264..3631)
                     /locus_tag="VGM46_08380"
     CDS             complement(2264..3631)
                     /locus_tag="VGM46_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013891549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY4192651.1"
                     /translation="MDSRPAPFVPPAPKPRTSPPSTLEMIRIVYRNPLELWGEPTYNE
                     PWISVTGVGGPLVIANDPGLIRHVLVDNAKNYKMATVRQKILRPILRDGLLTAEGQVW
                     KRSRKAMAPVFTPRHIFGFAQPMLKRTLEFVERYRDGATTDLAHDMTMLTYDILAETL
                     FSGEIAGEKGTFAQDIDRLLETMGRVDPLDLLRAPDWMPRLTRIRGRKTMAYFRKIVA
                     DTVTMRQDRLKRDLDGAPQDFLTLLLRAEGPGGLTRAEVEDNIITFIGAGHETTARAL
                     GWTLYCLAEAPWEREPIEREIDAVLASEPDPTKWLDAMPLTRAAFDEALRLFPPAPSI
                     NREPIEPETYNGLYIPPKAAVLVMPWVIHRHRKLWDWPDAFMPERFHPGNREKIDRFQ
                     YLPFGAGPRVCIGASFAMQEAIIALAILLSRFRFDSTAETKPWPVQKLTTQPQGGLPM
                     RVTRR"
     gene            3916..4488
                     /locus_tag="VGM46_08385"
     CDS             3916..4488
                     /locus_tag="VGM46_08385"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HEY4192652.1"
                     /translation="MKHQLSIIVGSTRPGRVGPVYAEWLETFATAHGGFAPVIHDLAE
                     FDLPLLDEPHHPRMGKYMNDHTKAWSKAVDGADAFVFVTPEYNYFTAPAIVNAVDYLS
                     REWAYKPAALLSYGGMSGGLRSVEALKPLLTAVRLMPIPEGVALPMHNNARDEKGAFQ
                     PGDHVLSSAKTMLDELVRWSEALKPMRQAG"
     gene            4647..>5003
                     /locus_tag="VGM46_08390"
     CDS             4647..>5003
                     /locus_tag="VGM46_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018327165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY4192653.1"
                     /translation="MSDQTDTANGAEAEYGADSIKVLKGLDAVRKRPGMYIGDTDDGS
                     GLHHMVYEVVDNAIDEALAGHADLVTVTLNPDGSVTVIDNGRGIPTDIHTGEGISAAE
                     VIMTQLHAGGKFDQNSY"
BASE COUNT          889 a         1573 c         1658 g          883 t
ORIGIN      
        1 gtaacgatcg cggaacggat gccggaaacc tgggccagcc tggtcgaggc caagggccac
       61 ggcgtggtcg acggcgaggt cctgtcgcgc ggcgaggaag ccgacgaatt cctgttgatg
      121 ggtctgcggc tggtggaagg catcgacctt gcccgctacg agcagttttc cggacgcggc
      181 ctgtcttcga cgcggctttc gacgcttcaa gacgaggggc tggtttcgcc catcggcaat
      241 tcgcgcctgc gtgccacgcc ggccggcatg atcgtgctgg acgcggtggt agccgatctg
      301 gcgcggtagt tagcactcga ccgatttgcc gccggctgcc tgcgctctag cgggatgggc
      361 ttttggcgcg gctttttttg cctgcggcca ttgatggcgt aggcttgccg gccccgctgt
      421 cgcggccggc gcgacaggac gtaaccgtat cgcgctggat catgccgggc atcgcactct
      481 cctagatacc ggcaaatcga ccggcatcta tttcgttcca cgacctattc gccgttccgg
      541 ttttgcaagc ttggccaagc cgtgccaaac agggcgaagc aaggggtgag catgccagcc
      601 gagacgacgc cacatcgcta tatgatcggg cagacggaaa tggtcgcccg gccgctggaa
      661 gccgcgcttt atctggtggc gacgcccatc ggcaatctcg gcgacatcac gctgcgcgcg
      721 ctggaaacgc tggccgccgc cgatgttctt gcctgcgaag atacgcgggt aagccgcgtg
      781 cttttggcgc gctatggcat ccgccgccgc accaccgcct atcacgagca caattcggcg
      841 gaggccgggc cgaagctgat tgcggcgctt gaagccgggc aaagcgtggc gctgatttcg
      901 gacgccggca cgccgctgat ttccgacccc ggctaccggc tggtcggcga ggcgctggaa
      961 aagaacatcc gcgtcgtgcc cattcccggc gcatcggcgc cgctggcagc gctcaccgcc
     1021 tccggcctgc cctccgacgc cttcctgttt gccggcttcc tgcccgtcaa gaccgggcaa
     1081 aagctggcgc gactgcaaga attgaagatc gtgccggcga cgctgatctt cttcgaatcg
     1141 ccacgccggc tggccgatac gctcgccgcc atggtcgagg cgctgggcgg cgagcgacgg
     1201 gcaaccatcg gacgcgaact gaccaagacg ttcgaggaga tgcgccccgg cgccctggcc
     1261 acgctggccg aacattatgc ggcagccgac acgccaaagg gcgaaatcgt catttgcgtc
     1321 ggccctcccg accatgcggt ggaagagcct gccgatatcg accggctgct cttgtcgctg
     1381 gccaaggaga tgccggcctc gaaggcggcg gcggaggccg caaagatgac cggcggccag
     1441 aagcaggcgc tttaccgccg gctgctggaa ctcagggatg ccgcagagga cgacggtgac
     1501 tgagagcgcc accgccaagc ggcgcaaatc ccatcagcgc gggcatcgcg gcgaagggct
     1561 ggcggcgatg gcgcttcggt tgaagggctt tcgcatcctt gcccgccgct accgcaccaa
     1621 gctcggcgaa atcgacctga tcgcgcggcg cggcgacctc gtgctgatcg tcgaggtcaa
     1681 ggcgcgccgg acgctgatcg aggcgatgca ggccattgcg tttgaatcgg aacggcgcat
     1741 cgagggcgcc gccgatctgt ggctttcgcg ccagcccgat tacggcaggt tgtcggtgcg
     1801 cttcgacatg gtggcggtgc tgccgtggcg ctggccggtg catgtggaaa atgcgtttca
     1861 tggccgaaac taggcgcttg tttcgggaaa tagcgctcgc ggacttgaaa ccacgcgcgc
     1921 tgccgtttat cggcgggtcg ccggcatcgg ctttgccggc tcttggagac gtaagatgac
     1981 ccttcccgac cgaatgagcg tcgacgagcg cagcccgttc tacaacgccg aggcgctgga
     2041 gcgcggcgtc ggcatcctgt tcaacggcaa ggagcgggaa gacgtcagcg aatattgcgt
     2101 cagcgagggc tggattcgcg tcgccacgcc gaaatccaag gaccgcttcg gcaatccgct
     2161 gaccatcaag ctcaagggcg aggtgaagcc cttcgtcaag gaatagccgg acttcccggc
     2221 tgatcgcttt ccggacataa accggctcca atctggaatt gcttcagcgc cgcgtcaccc
     2281 gcatcggcag gccgccctgc ggctgcgtgg tcaatttctg caccggccac ggctttgttt
     2341 cggcggtgct gtcgaagcgg aagcgcgaca gcaggatggc cagcgcgatg atcgcttcct
     2401 gcatggcaaa cgaagcgccg atgcagacgc gcggccccgc cccgaacggc agatactgga
     2461 aacggtcgat cttttcgcgg tttcccggat gaaagcgctc cggcatgaag gcgtccggcc
     2521 agtcccacag cttgcggtgg cggtggatga cccagggcat caccagcacg gcggctttcg
     2581 gcggaatgta aaggccattg taggtttccg gctctatcgg ctcgcggttg attgagggcg
     2641 cgggcggaaa aagccggagc gcctcgtcaa aggcggcgcg ggtaagcggc atcgcatcca
     2701 gccacttggt cgggtcgggt tcgctggcca gcaccgcgtc gatctcgcgt tcgatcggct
     2761 cgcgctccca cggcgcctcg gccaggcaat aaagcgtcca gccgagcgcg cgcgccgtcg
     2821 tctcgtggcc ggctccgatg aaggtgatga tgttgtcctc gacctcggcg cgcgtcagtc
     2881 cgcccggccc ttcggcgcgc aacaaaagcg tcaggaaatc ttgcggcgcg ccgtccagat
     2941 cccgcttcag cctgtcctgc cgcatcgtta ccgtgtcggc gacgatcttg cggaaatagg
     3001 ccatggtctt gcggccgcga atgcgggtca ggcgcggcat ccagtcgggg gcgcgcagca
     3061 ggtcgagcgg atcgacccgg cccatggttt ccagcaggcg gtcgatatcc tgcgcaaacg
     3121 tgcctttctc gccggcgatt tcacctgaga acagcgtttc ggcgagaatg tcataggtca
     3181 gcatcgtcat gtcgtgggca aggtcggtgg tcgcgccgtc ccgatagcgc tcgacgaatt
     3241 ccagcgtgcg cttcagcatc ggctgggcga agccgaagat gtggcgcggc gtgaacaccg
     3301 gcgccatcgc cttgcgcgag cgcttccaca cttggccttc cgccgtcagc agaccgtcgc
     3361 gcaggatcgg gcgcagtatc ttctggcgca ccgtcgccat cttgtagttc ttcgcattgt
     3421 cgaccagcac atggcggata aggccgggat cgttggcgat gaccagcggg ccgccgacgc
     3481 cggtgaccga aatccacggt tcgttgtacg tcggctcgcc ccacagttcg agcgggttgc
     3541 ggtagacgat gcggatcatc tccagcgtcg agggcggcga cgtgcgcggc ttcggcgccg
     3601 gcgggacgaa gggggctggt cggctatcca ttgtgcgctc cgctcaagag ctgaatgtag
     3661 ggtggagcga ggcttgcgtc catgcgaccg gccggcgcgg cgcctgcccg ccgccattgg
     3721 cggaaccgat gcctgtcata gaagccctgt gaagaaaccc cagcatggaa attccatcgg
     3781 gggggggaaa cgccatcgag aaacgatgtg ttcaaccaat ccaatccagt tcagccgagc
     3841 aaatgcttgc cctcgaccgg cgcagcgctt aggtgacgtt tgccaccgga taccgtcagc
     3901 catcaggagc gccccttgaa acatcagttg agcatcatcg tcggcagcac gcgccctggc
     3961 cgggtcggcc cggtctacgc cgaatggctg gagacatttg caacggcgca tggcggcttt
     4021 gcgccggtca tccacgacct cgccgaattc gatctgccgc tgctcgacga gccgcaccac
     4081 ccgcgcatgg gcaaatacat gaacgatcac accaaggcct ggtcgaaggc ggtggatggc
     4141 gccgatgcct tcgtgttcgt gacgcccgaa tataattact tcaccgcgcc ggccatcgtg
     4201 aatgccgtgg actacctttc gcgggaatgg gcctacaagc cggcagcact tcttagctat
     4261 ggcggcatgt cgggcggctt gcgttcggtg gaagcgctga agccgctgct gacagccgta
     4321 cggctgatgc cgattccgga aggggtggcg ctgccgatgc acaacaatgc gcgcgacgaa
     4381 aaaggcgcgt tccagcccgg cgaccatgtg ctttcgagcg ccaagaccat gctggacgag
     4441 cttgtgcgct ggagcgaggc gctgaagccg atgcggcagg ccggctagcc gcgccacggc
     4501 cactttcggc gggcgcttcg agcgcgctca aatgcctgca aaacttggaa aatcggctgc
     4561 aaaggcctat attcaggcaa gcgccgggct gcgattcgcg gcctggtttc caagcggcat
     4621 tcgcgccagc aaccgaacgg attttcatga gcgaccagac cgacaccgcc aatggcgcag
     4681 aggccgagta cggcgccgat tccatcaagg ttttgaaggg gttggatgcc gtgcgcaagc
     4741 ggccgggcat gtatatcggc gacaccgatg acggctctgg cctgcaccac atggtttatg
     4801 aggtggtcga caacgccatc gacgaggcct tggccggcca cgccgacctc gtcacggtga
     4861 cgctgaaccc cgacggttcg gtgacggtta tcgacaatgg gcgcgggatt ccaacggata
     4921 tccacaccgg cgaaggcatt tcggccgccg aagtcattat gacccagctt cacgcgggcg
     4981 gtaagttcga ccagaattcg tac
//
LOCUS       DASYCS010000258         3483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1806096,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000258 DASYCS010000000
VERSION     DASYCS010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1806096"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..959
                     /locus_tag="VGM46_08395"
     CDS             <1..959
                     /locus_tag="VGM46_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEY4192654.1"
                     /translation="NPGMVSWFVKQALLNLAGDLGFDFSEPAQDDREGWAALMEKAGV
                     KGIHIAERDTQRAAKPKPMGVFWNTWSVEGFISEGLQPAELGWGTHEKWMPENARTHS
                     EGSGAGIYLEQPGANTRVRTWCPTPGPQFGFLVTHNEAISIADFFTVRGKDGAAQYRP
                     TCHYAYHPCNDAVLSLHEMFGAEGKAQEVQHVLEEYELVDGVDELGVLLYGHARNAYW
                     YGSHLSLQEARRLAPYHNATGLQVTSAVVAGMVWALENPNAGIVETDEVDYRRCLEVQ
                     AQYLGPVEGYYTDWTPLDGRPGLFAEDIDKTDPWQFRNILVR"
     gene            1046..1426
                     /locus_tag="VGM46_08400"
     CDS             1046..1426
                     /locus_tag="VGM46_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192655.1"
                     /translation="MKLSRTTLNGALAMAAALALAACATSGPSTPLAGTAAPKGVEGS
                     WIDAQGTGLSTLSSGSFRTVATDTGQKLAEGSYVVTGATSVEINGTSLIRQSPIGFNC
                     LLVSTSQLNCTSSSGQNFVLTRRG"
     gene            1536..2591
                     /gene="hemH"
                     /locus_tag="VGM46_08405"
     CDS             1536..2591
                     /gene="hemH"
                     /locus_tag="VGM46_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEY4192656.1"
                     /translation="MNLSNANAARAIDPLTAGRPAVETGKVGVMLVNLGTPDGTGFKP
                     MWRYLREFLSDPRVIELNRAIWYPILYGLVLTTRPQKSGANYARIWNREKNESPLRTF
                     TRAQGQRLAQALGDLPNVVVDWAMRYGNPSTASVAQKLVEQGCDRIVCFPLYPQYSAT
                     TTATANDQLFRALMKMRRAPAVRSVPPYYDEPAYIDALASSIEKHLATLDFAPEVVIT
                     SYHGIPKPYSDKGDPYYFHCQKTTQLLRERLGWDDRRLIITFQSRFGAQEWLQPYTDK
                     TIEQLAKDGVKSIAVVNPGFSVDCIETLDEIGREAAETFHHAGGKNFTHIPCLNDSAE
                     GMAVIEAMVRRELSGWA"
     gene            2645..>3483
                     /locus_tag="VGM46_08410"
     CDS             2645..>3483
                     /locus_tag="VGM46_08410"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY4192657.1"
                     /translation="MPRKLDLRTGRPVWFAYRAPKVATDRLTRDVKTDVLIVGMGISG
                     AMIAEALTAQGRSVICIDRRGPLKGSTPATTALVQFEIDQPLTALSRMIGKAPAQQAW
                     RRSRIAVTNLRGRIEGLGIDCDPVGRQSLYLAGTTLGPSDLRAEADARREAGIAADYL
                     KPLQLKGRFGIDRDGAILSQENLALDPRKLTAGLLNKAAQRKAKFYSPVEAIKFQDGR
                     DEVIVGTRDGPTVTAKNVVLATGYELTDIVPAAAHQVISTWAIATKPQPRKLWPGAAL
                     IWEA"
BASE COUNT          672 a         1159 c         1062 g          590 t
ORIGIN      
        1 ccaaccccgg catggtgtcg tggttcgtca agcaggcgct gctcaatctc gccggcgacc
       61 tcggcttcga tttttccgaa ccggcgcagg atgaccgcga gggctgggcg gcgctgatgg
      121 aaaaagccgg cgtcaagggc atccatatcg ccgagcgcga tacgcagcgc gccgccaagc
      181 ccaagccgat gggcgtcttc tggaacacat ggtcggtgga aggcttcatt tccgaaggcc
      241 ttcagcccgc cgaactgggc tggggaacgc atgagaaatg gatgccggaa aacgccagga
      301 cgcatagcga aggctccggc gccggcatct acctcgaaca acccggcgcc aatacgcgcg
      361 tgcgcacctg gtgcccgacg cccggcccgc aattcggctt tctggtgacg cataatgagg
      421 cgatctccat cgccgatttc ttcaccgtgc gcggcaagga cggcgcggcg caataccgcc
      481 cgacctgcca ctatgcctac catccgtgca acgacgcggt actgtcgctg cacgagatgt
      541 tcggagccga aggcaaggcc caggaagtcc agcacgtgct tgaggagtac gagctggtcg
      601 atggcgtcga cgagcttggc gtgctgctct atggccacgc caggaacgcc tactggtatg
      661 gctcgcatct gtcgcttcag gaagcgcgcc ggcttgcgcc ttatcacaat gccaccggcc
      721 ttcaggtcac ctcggcggtg gtcgccggca tggtctgggc gctggagaac ccgaatgccg
      781 gcatcgtcga gacggatgag gtcgattacc gccgctgcct cgaagtacag gcgcaatatc
      841 tcgggccggt cgaaggctat tacaccgact ggacgccgct ggatggacgg ccgggcctgt
      901 ttgccgagga catcgacaag accgacccgt ggcagttccg caacatcctc gtgcgatagc
      961 gacaggctgc ccccacccgc atcccttgca atcgcccgga aatcgggcga ttgaaaagct
     1021 gcggaccgag gagaggacca ctcaaatgaa gctttcgcgc accacgctca acggcgctct
     1081 tgccatggcg gcggccctgg cgctggccgc ctgcgccaca agcgggccat ccaccccgtt
     1141 ggctggcact gctgcgccca aaggggtcga aggctcctgg atcgacgcac agggcaccgg
     1201 cctttccacg ctttcctccg gcagcttccg cacggtggcg accgataccg gccagaaact
     1261 cgccgaaggc agctatgtcg tcaccggcgc gacttccgtg gaaatcaacg gcacctcgct
     1321 gatccgccag tcgccgatcg gcttcaactg ccttttggtg tcgaccagcc agctgaattg
     1381 caccagttct tccgggcaaa actttgtgct gacccgccgc ggctaagcgg cagcggtcat
     1441 ttccccaccc cgaacaaccg gggtgcggcc acacgcggca ttgaaaccct gcccggcatc
     1501 gctactttcc agcgatgccc aaggaaatgc ccgccatgaa tttatccaac gccaacgccg
     1561 cccgcgccat cgatccgctt acggctgggc gccctgccgt tgaaacgggc aaggtcggcg
     1621 tcatgctggt caatctcggc acgcccgatg gcaccggttt caagccgatg tggcgctatt
     1681 tgcgggaatt cctgtccgat ccgcgcgtca tcgagcttaa ccgggcgatc tggtatccga
     1741 tcctgtacgg gctggtcctg accacgcgtc cgcaaaaatc aggcgccaac tacgctcgca
     1801 tatggaaccg cgagaagaat gaatcgccgt tgcgcacctt tacccgcgcg cagggccaaa
     1861 gactcgcgca ggcgcttggc gatctgccga acgtcgtggt cgactgggcg atgcgctatg
     1921 gcaacccgtc gacggccagc gtcgcgcaaa agctggtcga gcagggttgc gaccgcatcg
     1981 tttgctttcc gctctatccg caatattcgg caaccacgac ggcaaccgcc aacgaccagc
     2041 tgtttcgtgc gctgatgaag atgcgccgcg ccccggccgt ccgcagcgtg ccgccctatt
     2101 acgacgaacc ggcctatatc gacgcgctgg ccagttccat cgagaagcat ctggcgacgc
     2161 tcgattttgc tcccgaggtg gtcatcacct cctaccacgg catcccgaag ccctattccg
     2221 acaagggcga cccctattat tttcactgcc agaagacgac gcaattgctg cgtgaacggc
     2281 tgggctggga cgataggagg ctcatcatca ccttccagtc gcgctttggc gcgcaggaat
     2341 ggttgcagcc ctacaccgac aagacgatcg agcaattggc caaggacggc gtgaaatcca
     2401 tcgccgtggt caatccgggc ttctcggtgg attgcatcga gacgctggac gagatcgggc
     2461 gcgaagcggc ggaaactttc caccatgccg gcggcaagaa cttcacccac atcccctgcc
     2521 tcaatgacag cgccgagggc atggcggtga tcgaggcgat ggtgcggcgc gaactttccg
     2581 gctgggcctg acgcccggaa cgtcagcgcc gcatgaacgt tgaccgaaac cctcggagac
     2641 gttcatgccc cgcaaactcg atttgagaac cggccgtccg gtctggttcg cctaccgcgc
     2701 gcccaaagtt gccaccgaca ggctgacccg cgacgtgaaa accgacgtgc tgatcgtcgg
     2761 catgggcatc agcggcgcca tgattgccga ggcgctgacc gcacaaggcc gctcggtgat
     2821 ctgcatcgac cggcgcggcc cgctcaaggg ttcgacgccc gcgaccacgg cactggtgca
     2881 gttcgagatc gatcagccgc tgacggcgct gtcgcgcatg atcggcaagg cgcctgccca
     2941 gcaggcatgg cggcgctcgc gcattgccgt caccaacctg cgcggacgca tcgaaggcct
     3001 cggcatcgat tgcgaccctg ttggccggca atcgctctat ctggccggca cgacgctcgg
     3061 tccctcggac ttgcgcgccg aagccgatgc gcggcgggag gctggaatcg ccgccgatta
     3121 cctgaaaccc ctccagctca agggccggtt cggcatagac cgggacggcg ctatcctgag
     3181 ccaggaaaac ctggcgctcg atccacgtaa actgaccgct ggtctcctca acaaggcggc
     3241 gcagcgcaag gcgaaattct attcgccggt ggaggcgata aagttccagg atggtcgcga
     3301 cgaggtgatc gttggcacca gggacggacc caccgtcacc gcgaagaacg tcgtgttggc
     3361 gaccggctat gaattgacag acatcgttcc ggccgccgcg catcaggtca tctcgacctg
     3421 ggccatcgcc accaaaccgc agccgcgcaa gctatggccg ggcgccgccc tgatctggga
     3481 agc
//
LOCUS       DASYCS010000259         2123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3546174,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000259 DASYCS010000000
VERSION     DASYCS010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3546174"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="VGM46_08415"
     CDS             <1..665
                     /locus_tag="VGM46_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009756464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6537 domain-containing protein"
                     /protein_id="HEY4192658.1"
                     /translation="TLDERIARRAAFLSAYQNAAYGRRYADRIAALRAAESRLAAGST
                     VVTEAAAKNLFKLMAIKDEYEVARLYTDGSFARELSKQFQSYDSIEFHLAPPIMGRRD
                     TAGNPMKSSFGPWMMKAFRVLAALKGVRGTAFDVFGYSTERRLERRLLAQYEGDLDLI
                     GSRLSPASVEAAAALASVPALIRGFGHVKLAAVERAAAERARLVDRLDSAGAPPRLQA
                     AE"
     gene            790..>2123
                     /locus_tag="VGM46_08420"
     CDS             790..>2123
                     /locus_tag="VGM46_08420"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY4192659.1"
                     /translation="MAVSRKTELPDDVYIPFVETLFRDGFTLAIGILAQTLLISLVWA
                     KTGNPAYLAVVLCLVLVGIVRVWDMRNFAKLPPLTTRAQARKRENVYIVFGGMHGSVL
                     GAFCLVAITAAQDSFSETAAVCIALATAAGIAGRNYGSPRMVIILIITLTCPMSLGFI
                     LRGDFYHVTLGLLSTPFLLSIHRYADIVRDVLFTAISAEKRANSFAQRFNRALNTMSH
                     GLVMLGPDGRVVVANAEAAHLMSLKTPDALLGRTVHSLLMRGVAGNMLTPKDCRYVEA
                     QLTRALREGRDRKVLVGFSNGQHYEFSAREGSQELGVITFEDVTARVMAEDKIRFMAR
                     FDSLTGLANRAYFHEMVCEAMVVGDQDRLCGLAAFDLDDFKSVNDTLGHPIGDGLIYA
                     VADRLGALSAPGVQVSRFGGDEFMIYFDRVEDEIHLGAQMDDIFTALQGEIDV"
BASE COUNT          362 a          690 c          652 g          419 t
ORIGIN      
        1 aaacgctgga tgaacgcatt gcgcgccgcg cggcctttct ttccgcctac cagaacgcag
       61 cctatggccg ccgttacgcc gacaggatcg ccgccctgcg cgcggccgaa tcccggctcg
      121 ccgcaggctc caccgtggtc accgaagccg ctgccaaaaa cctgttcaag ctgatggcca
      181 tcaaggacga gtatgaggtg gcgcgcctct atacggacgg ctctttcgcc cgcgaactgt
      241 cgaagcagtt tcaaagctac gacagcatcg agtttcatct ggcgccgccg ataatggggc
      301 ggcgcgacac ggcgggcaac ccaatgaaat ccagcttcgg cccgtggatg atgaaggcat
      361 tccgcgttct ggcggcgctg aagggcgtgc gcggcacggc tttcgacgtg ttcggctaca
      421 gcaccgaacg gcgcttggag cgtcggttgc tggcgcagta tgaaggcgat ctcgatctta
      481 tcggcagtcg cctttctccc gcatcggtcg aggcagcggc agctctcgct tctgtgcccg
      541 ctctcattcg cggtttcggc catgtgaagc tcgccgccgt cgaacgtgcg gcggcggagc
      601 gggcaaggct ggtcgacagg ctggacagcg ccggtgcgcc accccggctc caggccgcgg
      661 agtgaccgat tacccattgt ttttcaacgg ataggcatgg attgagggtg ttcccggtct
      721 ccgacgccgg ggtctaagcc tttcttaagg cggatatggc aattcttggc tcccaaagcg
      781 ggaaccgacg tggccgtatc aagaaaaaca gagctcccgg acgacgttta tattccgttt
      841 gtggaaacgc ttttccgcga cggcttcacg ctcgccatcg gcattctggc ccagacgctt
      901 ctcatctcgc tggtatgggc caaaaccggc aatccggcct atctcgcggt tgtcctttgc
      961 cttgtgctgg tcggcatcgt gcgcgtgtgg gacatgcgca atttcgccaa gttgccgccg
     1021 ctcaccacgc gagcgcaggc gaggaagcgc gaaaacgtct acatcgtctt cggcggcatg
     1081 cacggcagcg tgctgggtgc cttctgcctg gtcgccatca ccgccgcgca ggacagcttt
     1141 tccgaaacgg ccgcggtctg catcgcgctg gcgacggcgg cgggcattgc cggccgcaac
     1201 tacggctccc cgcgcatggt catcatcctg atcattacgc tgacatgccc gatgtcgctc
     1261 ggtttcatct tgcgcggcga tttctaccac gtcacgctcg gcctgctttc cacgcccttc
     1321 ctgctcagca tccaccgcta tgcggacatc gtgcgcgatg tgctgtttac cgcgatttca
     1381 gccgaaaagc gggccaacag cttcgcgcag cgcttcaacc gcgcgctgaa caccatgtcg
     1441 cacggcctcg tcatgcttgg ccccgatggc cgcgtcgtgg tggctaacgc ggaagccgcg
     1501 catctgatgt cgctgaagac gcccgacgcg ctgctcggcc gcacggtcca ttcgctgctc
     1561 atgcgcggcg tcgccggcaa catgctgacg ccgaaagact gccgctatgt cgaggcgcaa
     1621 ttgacgcggg ccttgcgcga aggccgcgac cgcaaggttc tcgtcggctt ctcgaacggc
     1681 cagcactatg agttttcggc gcgcgagggc agccaggaac tgggcgtcat caccttcgag
     1741 gatgtcacgg cgcgggtaat ggccgaagac aagatccgct tcatggcccg cttcgacagc
     1801 ctgactggcc ttgccaaccg cgcttacttc cacgagatgg tctgcgaggc gatggtcgtt
     1861 ggcgaccagg accgcctctg cggcctggcg gcgttcgacc ttgacgactt caagagcgtc
     1921 aacgacacgc tcggccatcc catcggcgat gggttgatct acgccgtcgc cgatcggctg
     1981 ggcgcactct ccgcgcccgg cgttcaggtc agccgcttcg gcggcgacga attcatgatc
     2041 tatttcgacc gggtcgagga cgaaatccat ctcggcgccc agatggacga tatcttcacc
     2101 gcgttgcagg gcgaaatcga tgt
//
LOCUS       DASYCS010000260         5745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2384347,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000260 DASYCS010000000
VERSION     DASYCS010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 5745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5745
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2384347"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VGM46_08425"
     CDS             complement(<1..202)
                     /locus_tag="VGM46_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HEY4192660.1"
                     /translation="MSVEPEATVPPRRRLFVLLPLLIFLGLAGVFLSQLLSGHDSSEV
                     PSVLIGRPAPETHLPALQDTGLP"
     gene            complement(199..372)
                     /gene="ccmD"
                     /locus_tag="VGM46_08430"
     CDS             complement(199..372)
                     /gene="ccmD"
                     /locus_tag="VGM46_08430"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HEY4192661.1"
                     /translation="MSAHALYVTAAYAITAIVLAGLIGWVLLDQRARKRELAQLEAAG
                     IRRRSDKTGASKP"
     gene            complement(527..1192)
                     /gene="ccmB"
                     /locus_tag="VGM46_08435"
     CDS             complement(527..1192)
                     /gene="ccmB"
                     /locus_tag="VGM46_08435"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEY4192662.1"
                     /translation="MLALYLRDLKLSVRAGGGALTGVIFFLAVIVTIPFGVGPDLNLL
                     ARIGPAILWTGALLASLLGLDRLFQAEREDGSLDLLVLAGDRHMLALTVLVKCLAHWT
                     ASVLPLVIAAPLLGLFMNMDPLGIGATTLTLLVGTPAIAFIGAVGAAVAVALPRGGML
                     ISVLILPLTIPVLIFGVSASYGAVADPDPFLPPFLILAALTLFFAVIGPLAAALALRG
                     GAD"
     gene            complement(1194..1814)
                     /gene="ccmA"
                     /locus_tag="VGM46_08440"
     CDS             complement(1194..1814)
                     /gene="ccmA"
                     /locus_tag="VGM46_08440"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HEY4192663.1"
                     /translation="MRLIAENLSGERGGEPVFSGINFSLSNSEALVVTGPNGSGKSTL
                     LRIVAGLLPASGGKARLEGGGEAFVSIASACHYLGHQNAMKPTLSVTENLAFWRDFCG
                     ATELGIDDALDAVGLAGIGHLPFGFLSTGQRRRAAIARLLVSHRPLWLLDEPTAGLDK
                     ASEKRLSALMTAHCRNGGIIIAATHLPLGINAQELAMGDAAPSWGE"
     gene            2096..4786
                     /gene="acnA"
                     /locus_tag="VGM46_08445"
     CDS             2096..4786
                     /gene="acnA"
                     /locus_tag="VGM46_08445"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEY4192664.1"
                     /translation="MSKSLDSFNCRRTLVAGEAEYVYYSLIEAEKNGLTGIAQLPYSM
                     KVLLENLLRNEDGRTVTKASIKAVADWLTDKGTAGVEIAYRPARVLMQDFTGVPAVVD
                     LAAMRDAMVSLGGDPQKINPLVPVDLVIDHSVIVDEFGTPRALANNVALEYARNEERY
                     KFLKWGQQAFRNFRVVPPGTGICHQVNLEYLGQVVWTNAEGDENTAYPDTCVGTDSHT
                     TMINGLGVLGWGVGGIEAEAAMLGQPVSMLLPEVIGFRLTGKVKEGVTATDLVLTVTQ
                     MLRKKGVVGKFVEFFGPGLSNMTLADRATIGNMGPEYGATCGFFPVDAETIRYLNMSG
                     RSEARIALVEEYSKAQGMWRDNSSPDPVFTDMLELDLGEVVPSMAGPKRPEGRVALEG
                     IPAGFVKAMDTEYKKAAEISKRYQVEGEDFDLGHGDVVIAAITSCTNTSNPSVLIGAG
                     LLARNANRLGLKQKPWVKTSLAPGSQVVAEYLEKSGLQKELDQVGFNLVGFGCTTCIG
                     NSGPLPAPISRTINEKGLIGAAVLSGNRNFEGRVSPDVQANYLASPPLVVAHALAGTV
                     TKDLTTEPIGEGRDGKPVYLRDIWPSSAEIQEFIEKNVTRELFARKYADVFKGDENWQ
                     NVKAPEGQTYAWDDSSTYVQNPPYFAGMTTGFGTIADIKGARVLGLFGDKITTDHISP
                     AGSIKAASPAGKYLTDHGVGVADFNQYGTRRGNHEVMMRGTFANIRLRNHMLGENGRE
                     GGYTIHYPSKEEESIYDAAMRYKQEGVPLVVFAGVEYGNGSSRDWAAKGTSLLGVRAV
                     IAQSFERIHRSNLVGMGVVPFTFEEGTSWASLNLKGDELVEIDGLSSIKPRQTMMAKV
                     TYGDGTVKEVPILCRIDTLDELDYFKNGGILQYVMRDLAA"
     gene            4956..5729
                     /locus_tag="VGM46_08450"
     CDS             4956..5729
                     /locus_tag="VGM46_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEY4192665.1"
                     /translation="MAFGNPSRLAATALAFFVYADTGFAGQPAPAKDDAPLGVVELFT
                     SQGCSSCPPADELFTDLAARKDIVALSYHVDYWDYLGWKDTLSSKKNTERQYAYMQSF
                     RTRSVYTPQAVINGRTHVNGANRAAVDATLADMDKASKGMKVAVNVTHEGDGVVIEAG
                     NAAANWSGPSNAHVIVVYFEAPQTVAIGRGENGGRSETYWNAVYDIQTAGMWHGKTQR
                     YELPMSEVSKKGGSAVLLQAVGKDGLPGPILGAAMIRKP"
BASE COUNT         1129 a         1816 c         1848 g          952 t
ORIGIN      
        1 ccggcaggcc ggtatcctgc aacgcgggaa gatgcgtttc gggcgcgggc cggccaatca
       61 gcactgaggg cacttccgaa ctgtcgtggc cggaaagcag ttgcgacagg aacacgccgg
      121 caagtccaag gaaaatcagc agcggcagca aaacgaaaag ccggcgcctg ggcggcacgg
      181 ttgcctctgg ttcgacgctc atggcttgga cgctcccgtc ttgtcggagc ggcggcggat
      241 gccggcggct tccagttgcg ccagttcgcg cttgcgggcg cgctggtcga gcagcaccca
      301 gccgatcaac ccggccagga cgatggcggt aatggcatag gccgccgtca cgtagagcgc
      361 atgggcgctc atgcgagatg cctcacgcct gtgttgatcc tcatcgcgcc gcccgctctt
      421 tccatgtctt cccttagcgt ccggccgctg gccgtgcaat atttggcctt gcggcttggt
      481 gctttggcct ggcggctttg gcggcggatg aacgcgaagt gatccgtcag tcggcgccgc
      541 cgcgcagcgc aagggctgcg gcgagcggac cgatcacggc aaagaacagg gtcagcgcgg
      601 caagaataag gaaggggggc aggaaggggt ccggatcggc gaccgcgcca tagctcgccg
      661 acacgccgaa gatcagcacc ggaatggtca gcggcagtat cagcacggaa atcagcatgc
      721 cgccgcgcgg cagcgccacc gccacggcgg cgccaacggc gccgataaag gcgatggccg
      781 gcgtgccgac aagcagggtc agcgtggtgg cgccgatgcc gagcgggtcc atgttcatga
      841 acaggccgag caagggcgcg gcgatcacca gcggcagcac gcttgccgtc cagtgcgcca
      901 ggcatttcac cagcacggtc agcgccagca tgtggcggtc gccggccagc accagaagat
      961 cgagcgagcc gtcctcgcgc tcggcctgga acaggcggtc gaggccgagc agcgacgcta
     1021 gcagcgcgcc ggtccacagg atggccgggc cgatgcgggc tagcaggttg agatccggcc
     1081 cgacgccgaa ggggatggtg acgatgacgg cgagaaagaa gatgacgccg gtcagcgcgc
     1141 cgccgccggc acgcacggaa agcttcaggt cgcggagata gagggccagc actttactcg
     1201 ccccacgatg gagcagcatc gcccatcgcc aactcctgcg cgttgatgcc gagcggcaaa
     1261 tgcgtggcgg cgataatgat gccgccgttg cggcaatgcg ccgtcatcag ggcggagagg
     1321 cgcttttccg aagccttgtc cagtccggcg gtcggctcgt ccagcagcca aagcgggcgg
     1381 tgtgaaacca gcaagcgggc gatggcggcg cgccgccgct gtcccgtcga cagaaagccg
     1441 aacggcaaat ggccgatgcc ggcgaggccg accgcatcga gggcgtcatc gatgcccagc
     1501 tccgtcgcgc cgcagaaatc gcgccagaag gccaggtttt ccgtgacgct cagcgtcggc
     1561 ttcatggcgt tctgatggcc gagatagtgg caggccgagg caatcgagac aaacgcctcg
     1621 ccgccgcctt ccaggcgcgc cttgccgccg gaagccggca gcagcccggc gacgatgcgc
     1681 agcagggtgg atttgccgga gccgttgggg ccggtcacga ccagcgcctc actgtttgac
     1741 agcgaaaagt tgatgccgga aaacaccggc tcgccgccgc gttcgccgct cagattctcg
     1801 gcgatcagcc gcatcgattt cccttcgccc gaagtccggc agacatatgc gcgcacaggc
     1861 gcaaatttgg caaatctgtg tctagaacta ttccagaaaa atctctatat ccctcgcatc
     1921 cgtaccagcg gtcggtatgg gaaacgaatt tgcgccgaac cagcgcgcgc gccgccttga
     1981 acggctcgga cgcttgggaa cggacagcgg aaatggccgt acccgcacct ttgcgcgtga
     2041 ttgcatgcca tcggagtccg ttccttaggc tgaacagacc agaatgggat cgcacgtgtc
     2101 caaatctctc gatagtttca attgccgccg cacccttgtc gccggggaag cggaatatgt
     2161 ctattacagc ttgatcgagg ccgaaaagaa cggcctgacc ggcatcgcgc aactgcctta
     2221 ttcgatgaag gtgctgctgg agaacctgct gcgcaacgaa gacggccgca ccgtcaccaa
     2281 ggcttcgatc aaggcggtgg ccgactggct gaccgacaag ggcaccgccg gcgtcgagat
     2341 cgcctatcgc ccggcgcgcg tgctgatgca ggacttcacc ggcgttcccg ccgtcgtcga
     2401 ccttgccgcc atgcgcgacg ccatggtttc gctgggcggc gatccgcaga agatcaatcc
     2461 gctggtgccg gtcgacctgg tcatcgacca ttcggtgatc gttgacgagt tcggcacgcc
     2521 gcgcgcgctg gccaacaatg tcgcgctgga atatgcgcgc aacgaagagc gctacaagtt
     2581 cctgaaatgg ggccagcagg cgttccgcaa tttccgcgtc gtgccgcccg gcaccggtat
     2641 ctgccaccag gtcaacctcg aatatctggg tcaggtcgtg tggaccaacg cggaaggcga
     2701 cgaaaacacc gcctaccccg acacctgcgt cggcaccgat tcgcacacca cgatgatcaa
     2761 cggcctcggc gtgctcggct ggggcgtcgg cggcattgag gcggaagcgg cgatgctcgg
     2821 ccagccggta tcgatgctgt tgcctgaagt catcggcttc cggctcaccg gcaaggtcaa
     2881 ggaaggcgtc accgccaccg atctcgtgct gaccgtgacg cagatgctgc gcaagaaggg
     2941 cgtcgtcggc aagttcgtcg agttcttcgg tccgggcctg tcgaacatga cgctggccga
     3001 ccgcgccacc atcggcaata tgggtccgga atatggcgcg acctgcggct tcttcccggt
     3061 cgatgccgaa accatccgct atctcaacat gtccggccgc agcgaagccc gcatcgcgct
     3121 ggtggaggaa tattccaagg cgcaggggat gtggcgcgac aattcatcgc ctgatcccgt
     3181 cttcaccgat atgctggaac tcgacctggg cgaagtggtg ccgtcgatgg ccggcccgaa
     3241 gcgtccggaa ggccgggtgg cgctggaagg aattccagcc ggtttcgtca aggcgatgga
     3301 caccgaatac aagaaggccg ccgaaatctc caagcgctac caggtcgagg gcgaggactt
     3361 cgaccttggc catggcgacg tggtcatcgc ggccatcact tcctgcacca acacctcgaa
     3421 ccccagcgtg ctgatcggcg cgggcctgct tgcccgcaac gccaaccggc ttggcctcaa
     3481 gcagaagcca tgggtgaaga cctcgctggc tcccggcagc caggtcgttg ccgaatatct
     3541 ggaaaagtcc ggcctgcaaa aggaactcga ccaggtcggc ttcaacctcg tcggcttcgg
     3601 ctgcaccacc tgcatcggca attccgggcc gctgccggcg ccgatctcca ggacgatcaa
     3661 cgagaagggg ctgatcggcg ctgcggtgct ttcgggcaac cgcaatttcg aaggccgcgt
     3721 ctcgcccgac gtgcaggcga actacctcgc ttcaccgccg ctggtggtgg cgcacgcgct
     3781 ggccggcacg gtgaccaagg acttgaccac cgagccgatc ggcgagggcc gcgacggcaa
     3841 gccggtctac ctgcgcgaca tctggccgtc ttccgccgaa atccaggagt tcatcgagaa
     3901 gaacgtcacg cgcgagcttt tcgcccgcaa atatgccgac gtcttcaagg gcgacgagaa
     3961 ctggcagaac gtcaaagcgc cggaaggcca gacctacgcc tgggacgaca gctcgaccta
     4021 tgtgcagaac ccgccctatt tcgccggcat gaccaccggt ttcggcacca tcgccgacat
     4081 caagggcgcg cgcgtgctcg gcctgttcgg cgacaagatc accaccgacc acatctctcc
     4141 ggccggttcg atcaaggcgg cgtcgccggc cggcaaatat cttaccgatc atggcgtcgg
     4201 cgttgctgac ttcaaccagt acggcacgcg ccgcggcaat catgaagtga tgatgcgcgg
     4261 cactttcgcc aacatccgcc tgcgcaacca tatgctgggc gaaaacgggc gcgagggcgg
     4321 ctacacgatc cactatccgt cgaaggaaga ggaatcgatc tacgacgccg ccatgcgcta
     4381 caagcaggaa ggcgtgccgc tggtcgtctt cgccggcgtc gaatacggca atggctcgtc
     4441 gcgcgactgg gcggccaagg gcaccagcct gctcggcgtg cgcgccgtca tcgcccagtc
     4501 gttcgagcgt atccaccgtt cgaacctggt cggcatgggc gtcgttccgt tcaccttcga
     4561 ggaaggcacg tcgtgggcct cgcttaatct caagggcgac gaactcgtcg agatcgacgg
     4621 cctttcctcg atcaagccgc gccagacgat gatggccaag gtgacctatg gcgacggcac
     4681 ggtgaaagag gtgccgatcc tgtgccgcat cgatacgctg gacgaactcg actacttcaa
     4741 gaatggcggc atcctgcaat atgtgatgcg cgacctggcc gcgtaagcag cttttaaagc
     4801 cgacaaatcg gatcgccggg gcgaaagctc cggcgatttt gtttttcggg gcgaaaaatt
     4861 tcaccgcaac gtgacttccc gttgactgcc ggttctgcca catattttag cgataagaag
     4921 aaaagccggc atcaccggcg ggaatcggaa cggtcatggc atttgggaat ccttcgcggc
     4981 tggcagcgac agcgctggcc tttttcgtct atgccgacac gggctttgcc ggccagccgg
     5041 cgcctgcgaa ggatgacgcg ccgctgggcg tggtcgaact gttcaccagc cagggttgta
     5101 gctcatgtcc gccggcggac gaactgttca ccgatcttgc cgcgcgcaag gacatcgtgg
     5161 ctctctccta tcatgtcgac tactgggact atctcggctg gaaagatacg ctgagcagca
     5221 agaagaacac cgagcgccag tacgcctaca tgcagtcgtt ccgcacccgt tccgtctata
     5281 cgccgcaagc cgtcatcaac ggtcgtaccc atgtcaacgg cgccaaccgc gcggcggtcg
     5341 atgcaacgct tgccgacatg gacaaggcgt ccaagggcat gaaggttgcc gtcaacgtca
     5401 cccacgaggg ggacggcgtg gtgatcgagg ccggcaatgc ggcggcgaac tggtcgggac
     5461 cgtccaacgc ccatgtcatc gttgtctatt tcgaagcgcc gcagacggtc gccatcggca
     5521 ggggggaaaa tggtggccgc agcgagacat actggaacgc cgtttacgat atccagaccg
     5581 ccggcatgtg gcatggcaag acgcagcgct atgaattgcc gatgagcgag gtttccaaaa
     5641 agggcggcag cgccgtcctg ttgcaggcgg tcggcaagga cggcctgcct ggacctatcc
     5701 ttggcgcggc gatgatccgc aagccataga ggcttcgcct ccgtc
//
LOCUS       DASYCS010000261         3396 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_354986,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000261 DASYCS010000000
VERSION     DASYCS010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354986"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1143
                     /locus_tag="VGM46_08455"
     CDS             <1..1143
                     /locus_tag="VGM46_08455"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008875678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HEY4192666.1"
                     /translation="QSSLGSGVLVDPTGIVVTNFHVIKDADEVKVATSDGREFTSKVL
                     LKDETLDLAVLKIDSDKPFPVVVIGDSDALEVGDLVLAIGNPFGVGQTTTSGIVSALA
                     RSHIGISDSGFFIQTDAAINPGNSGGALINMGGQLVGINTAIFSRSGGSIGIGFAIPS
                     NMVRAFTEAAKAGRDFFERPYLGAQFEPVTPQIAESLGMEAPAGALVSSVIEGGPATK
                     AGLKPGDVVLKMNGTALESVDALGYRLATQSIGAKATFDVLSKGQQQTLDIVLERAPE
                     GANPATVALRGRSPFSGAKVAELSPRLAQRLGMRTDLTGVAVVGIDGGSPAAGFGFQA
                     GDIVREVNGVTIDNPKALEQVAAQNTRWWRFTIERDGQTLHQVLRY"
     gene            1153..2463
                     /locus_tag="VGM46_08460"
     CDS             1153..2463
                     /locus_tag="VGM46_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008875679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEY4192667.1"
                     /translation="MGDLFSSGEPEKAPAGRPLADRLRPKNLEEVVGQEHLAGPDGAL
                     TRMIASGSLGSMIFWGPPGTGKTTVARLLAGETSLAFEQISAIFSGVADLKKVFEAAR
                     LRRANGRQTLLFVDEIHRFNRAQQDSFLPVMEDGTVVLVGATTENPSFELNAALLSRA
                     RVLVFHSLGEDSISKLLARAEEMEGRPLPLDDGARAVLLRMADGDGRASLTLAEELWR
                     AAKPGEIFDADGLQRIVQRRAPVYDKGQDGHYNLISAPHKSVRGSDPDAALYYLARMF
                     DAGEDPLYLGRRLVRMAVEDIGLADPQALVVANAAKDAYDYLGSPEGELAFAQACVYL
                     ATAPKSNAVYTAFKAATRAAKEYGSLLPPKHILNAPTKLMKEEEYGSGYRYDHDEPEA
                     FSGQDYFPEKMGRHVFYDPPERGFERDIRKRLDYWSKLRKERER"
     gene            2696..2992
                     /locus_tag="VGM46_08465"
     CDS             2696..2992
                     /locus_tag="VGM46_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192668.1"
                     /translation="MNIYGSSSVPIDRNPAASGRSTYASANNVRADETKREHGDVDAA
                     RSTALSALKNDRFGTMLKQLSDPGTSGALMTMKPDADGPGADYNSVLSSYAENE"
     gene            complement(3287..>3396)
                     /locus_tag="VGM46_08470"
     CDS             complement(3287..>3396)
                     /locus_tag="VGM46_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019858688.1"
                     /note="catalyzes the formation of methionine from
                     L-homocysteine and S-adenosyl-L-methionine; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEY4192669.1"
                     /translation="VETIIERIGPLRNKAAQENIAETAQGRRERRRTRV"
BASE COUNT          623 a         1023 c         1104 g          646 t
ORIGIN      
        1 caatcctcgc tcgggtccgg cgtgctcgtc gatccgaccg gcatcgtcgt caccaatttc
       61 cacgtcatca aggatgccga cgaggtcaag gtggccacgt cggacgggcg tgagttcacc
      121 agcaaggtgc tgctcaagga cgagacgctt gatcttgccg tgctcaagat cgacagcgac
      181 aagccgttcc cggtcgtcgt cataggcgat tcggatgcgc tggaagtggg cgatctggtg
      241 ctggcgatcg gcaatccgtt cggcgtcggc cagacgacga ccagcggcat cgtctcggcg
      301 ctggcgcgca gccatatcgg catttcggat tccggcttct tcatccagac ggacgccgcc
      361 atcaatccgg gcaattccgg cggcgcgctc atcaatatgg gcgggcagct tgtcggcatc
      421 aacaccgcca ttttcagccg cagcggcggc tccatcggca tcggctttgc cattccctcc
      481 aacatggtgc gcgccttcac cgaggcggcc aaggccggcc gggacttctt cgagcgtccg
      541 tatctcggcg cgcagttcga gccggtgacg ccgcagatcg ccgaatcgct cggcatggaa
      601 gcaccagcgg gcgcgcttgt ctcatccgtg atcgagggcg gtcccgccac caaggccgga
      661 ttgaagccgg gcgacgtggt gttgaagatg aacggcacgg cgctggagag cgtggatgcg
      721 ctgggctacc ggctggcgac gcaatcgatt ggcgccaagg cgaccttcga cgtgctgagc
      781 aaaggccagc agcagacgct cgacatcgtg ctggagcgcg cgccggaggg cgcgaacccg
      841 gctaccgttg cgctgcgtgg gcgcagcccg ttctcgggtg ccaaggtggc tgaactgtcg
      901 ccgcgcctgg cgcagcggct tggaatgcgc accgacttga ccggcgttgc ggtggttggc
      961 atcgatggcg gctcgcctgc cgcgggcttc ggcttccagg ccggcgatat cgtgcgcgag
     1021 gtcaatggcg tgacgatcga caatcccaag gcgctggaac aggtcgcggc gcagaacacg
     1081 cgctggtggc gctttacgat cgagcgggat gggcagacac tgcaccaggt cttgcggtat
     1141 tgacgagttt tcatgggtga tctgttcagt tccggcgagc cggaaaaggc gcctgccggg
     1201 cggcctttgg cagacaggct gcgtccgaag aaccttgagg aggtcgtcgg gcaggagcat
     1261 ctggctggac ccgatggcgc gctgacgcgc atgatcgcct caggttcact cggatcgatg
     1321 atcttctggg gaccgccggg gacgggcaag accacggtgg cgcggcttct ggccggcgag
     1381 accagccttg ccttcgagca gatatcggcg atcttttcgg gtgtcgccga tctgaagaaa
     1441 gtgtttgagg ccgcaaggct gcgccgcgcc aacggccgcc agacgctgct gttcgtcgat
     1501 gagattcatc gcttcaaccg cgcgcagcaa gacagcttcc tgccggtgat ggaggacggc
     1561 acggtcgtcc tcgtcggcgc gacgacggag aatccatcct tcgaattgaa cgccgcgctt
     1621 ctgtcgcggg cgcgtgtgct ggtgttccat tcgcttggcg aggacagtat ttcaaagctg
     1681 ctggcgcggg ctgaggaaat ggaaggccgg ccgctgccgc tggacgacgg ggcgagggcg
     1741 gtgctcctgc gcatggccga tggcgatggc cgtgcctcgc tgacgcttgc cgaggaattg
     1801 tggcgcgccg ccaagcctgg agaaatattc gacgccgatg ggttgcagcg catcgtgcaa
     1861 cgccgcgcgc cggtctacga caagggccag gatggtcact acaacctgat ttcggcgccg
     1921 cataaatcgg tgcgcggttc cgacccggat gcggcgctct attatctggc gcggatgttc
     1981 gatgccggcg aagacccgct ctatcttggc cggcgtctgg tgcgcatggc ggtcgaggat
     2041 atcggcctgg ccgatccgca agcgcttgtc gtcgccaatg cggccaagga cgcctatgac
     2101 tatctcggct cgccggaagg tgaactcgcc ttcgcgcagg cttgcgtcta cctcgccacc
     2161 gcgcccaaat ccaacgccgt ctatacggca ttcaaggcgg ccacccgcgc cgccaaggaa
     2221 tatggctcgc tgctgccgcc caagcacatt ctcaatgcgc cgaccaagct gatgaaggaa
     2281 gaggaatacg gctcaggcta tcgctacgac catgacgagc cggaagcgtt ttccgggcag
     2341 gactattttc ccgaaaagat gggccggcat gtcttttacg atccgcccga acgcggcttc
     2401 gagcgcgata ttcgcaagcg gctcgattac tggtcaaagc tgcgcaaaga acgagaacgc
     2461 tagagcggtt ctggttttta ccgaatcgac aaaaccgctc tatctctttg tttggctgca
     2521 tttccgaacg caaaaccgtt tcacactttt gctggaaatg ctctagcata gcgtcatgac
     2581 ggggcgccgt gacgacgatt tttgacgttc gctaaaatgc gagggattgg ctgcaccggt
     2641 tttcaacaag gccgccatta gagatggcga tatcaaggtt ttgagggaaa ccgcagtgaa
     2701 tatttacggc tcgtcgtcag ttccgatcga tagaaaccct gccgcatccg gcaggtcgac
     2761 ctatgcgtcg gcaaataatg taagggccga cgagaccaag cgtgaacatg gcgacgtgga
     2821 tgcggcgcgc agtaccgccc tgtcggcgct gaagaacgac cgcttcggga cgatgctgaa
     2881 gcaactatcc gaccccggca catccggcgc gctgatgacc atgaaacccg acgccgatgg
     2941 cccgggcgcg gattacaatt cggtgctgtc gagctacgcc gagaatgaat aaggccgact
     3001 cactggcgta gagctgtttc gccgttagac agggtcactc tgtttgggtg ggctgtgagg
     3061 cgggtcgcga gggtcgatgc ggttcagcgg acgagcgagc caacaaagcg cacgtccctg
     3121 tcccatccgg ccgctcggcc agagtaccgc aatggcccga ccgatccggg tcgaatccct
     3181 tggccttcat ctcaacaaca ccgctaggtg cgatcttcga caatggcccc ggttttgtcc
     3241 cggaccgtta ccggcagaat gcttcgatcc aatcggtaaa gcggccctag acgcgggtac
     3301 ggcgtcgttc gcgacgccct tgcgccgttt cggcgatatt ttcctgcgcc gccttgttgc
     3361 gcagcggccc gatgcgctcg atgatggtct cgacgc
//
LOCUS       DASYCS010000262         5360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2530410,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000262 DASYCS010000000
VERSION     DASYCS010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 5360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5360
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2530410"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1080
                     /locus_tag="VGM46_08475"
     CDS             <1..1080
                     /locus_tag="VGM46_08475"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019859096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEY4192670.1"
                     /translation="TGHAAASIGTLGIVTAQGTAYGTMTTPDPVALHRELDSLAAAGI
                     THAAIEASSHGLDQRRLDGLRIVAAGFTNLGRDHMDYHPTMEEYHRAKLRLFDRLLPK
                     GAPAVIFADDAWSEPTVKAALAAGLDVLTVGRHGSFLRLKRVEHERSRQRAEVEADGV
                     LYEIDLPLAGDFQIANALVSAGLAISTGTPVATAIAALEKLKGAPGRLDLIGTTANGA
                     PVYVDYAHKPDALENVLSAVRPFTTGKVVVVFGCGGDRDRGKRPIMGEIATRLADVTI
                     VTDDNPRSEAPETIRAAILAAAPGAIEIGDRRKAIHEAVAMLHAGDTLIVAGKGHEEG
                     QTIGSETFHFSDHEEVRAALKDRAA"
     gene            1077..2498
                     /locus_tag="VGM46_08480"
     CDS             1077..2498
                     /locus_tag="VGM46_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamyl-2,
                     6-diaminopimelate--D-alanyl-D-alanine ligase"
                     /protein_id="HEY4192671.1"
                     /translation="MNRLWTSEALVAAMDGRPIGSMPEGIGGISIDTRSLQPGDAFFA
                     IKGETMDGHDFATAAIKAGAGVLVVAEGKLPALGRLTAPMIVVPDVLEALQKLGIAAR
                     ARSRAKIIAVTGSVGKTSTKEALRHALSAVGKVHASDKSFNNHWGVPLTLARMPDDCD
                     FAVFEIGMNHPDEIRPLVKMVRPHIAVVTLIAAAHLGFFRNLEEIAKAKAEIFEGLEP
                     GGTALLNRDDPRSKLLEKLAVAAGVENIAGFGENARSTYRLIDCALHADHSVIAVKIG
                     GEEMIVRIGAPGRHIAQNALAVLGAAHLAGADVAAVALALGSLSAEAGRGKRHILRHP
                     EGPFTLIDESYNANPASMAAALALLDATPVSGQGRRIAVLGDMLELGAHGPKLHAGLA
                     EQIVGTGIQTVFLGGPEMKALADVLPGDIATAYREGADELKPVLLGTVGPGDVVMVKS
                     SKSIGFSKLVEALLKKFPAQAEG"
     gene            2522..3604
                     /gene="mraY"
                     /locus_tag="VGM46_08485"
     CDS             2522..3604
                     /gene="mraY"
                     /locus_tag="VGM46_08485"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEY4192672.1"
                     /translation="MFTLLVDFADRITVFNVFRYITFRTGGALITSALIVFIFGPTII
                     NSLRLRQGKGQPIRADGPQTHFKKAGTPTMGGLMILTGIIISALLWANLRNVYVWVVL
                     LVTVGFGAIGFYDDYLKVTKQSHLGFSGKARLGLEFLIAGIASWLIMHAGQEPFSSSL
                     TFPFAKEFLINLGWFFIPFGAFVMVGAGNAVNLTDGLDGLAIVPIMIAAASFGVIAYL
                     SGNAVFAEYLQIHFVPGTGELAVVLGAVIGAGLGFLWFNAPPAAIFMGDTGSLALGGL
                     IGTVAVATKHEIVLAIVGGLFVVEIFSVIIQVGYFKMTGKRVFLMAPIHHHFEKLGWT
                     ESQVVIRFWIIAVILALVGLSTLKLR"
     gene            3620..5020
                     /gene="murD"
                     /locus_tag="VGM46_08490"
     CDS             3620..5020
                     /gene="murD"
                     /locus_tag="VGM46_08490"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013894668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEY4192673.1"
                     /translation="MIPAATFAGKRVSLFGLGGSGIATARALMEGGADVLAFDDNPGS
                     VAKAQAEGIPTGDLRGADWSRFSSFVLSPGVPLTHPKPHWTVELARGAGVEIIGDVEL
                     FARERIARAPDAPFIAITGTNGKSTTTALTAHILKAAGRDAQMGGNIGRAVMTLDPPQ
                     AGRHYVVECSSYQIDLAPSINPTAGVLLNLTPDHLDRHGTMQHYAAIKERLVAGSDTA
                     IIGIDDSWCAQIADRLERVGKEVIRISKRLPLTDGYFADGTDLMEAVQGRYSRIGFLE
                     GIGSLRGQHNAQNALAAVAACLKVGLELGEIQSGLESFPGLAHRMEQVGRKGHVLFVN
                     DSKATNADAAAPALSSFPRIYWIAGGLPKEGGIEPLRGLFPRIAKAYLIGEAAPAFSA
                     TLGETVPYEISGTLAAAVEHAAADAEKDNSGEVVVLLSPACASFDQFKNFELRGEFFR
                     QAARAIDGVKVIGEIR"
     gene            5020..>5360
                     /locus_tag="VGM46_08495"
     CDS             5020..>5360
                     /locus_tag="VGM46_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEY4192674.1"
                     /translation="MAQRSRLDKSPVATWWWTIDRWFLAAFLSLMGLGIVLSFAASPA
                     VAQRIGLDPFHFATRQIIFTIPALGVMLAVSFLESRQIRRMALIMLAIMLVLMVVVLY
                     VGVEVKGARRWV"
BASE COUNT          906 a         1669 c         1783 g         1002 t
ORIGIN      
        1 accggccacg ccgcggcgag catcggcacg ctcggcatcg tcaccgcgca gggcaccgcc
       61 tacggcacca tgacgacgcc cgatccggtc gcgctccacc gcgagctgga cagcctcgcg
      121 gctgccggca tcacccatgc cgcgatcgag gcgtcgagcc acgggctcga ccagcgccgg
      181 ctcgacggcc tgcgcatcgt cgccgcgggc ttcaccaatc tcggccgcga ccacatggat
      241 taccatccga ccatggagga gtatcaccgc gccaagctgc ggctgttcga taggctgctg
      301 cccaagggcg cgccggccgt catcttcgcc gacgatgcgt ggtcggagcc gacggtcaag
      361 gcggcgctag ctgccgggct ggacgtgctg acggtcgggc ggcatggctc tttcctcagg
      421 ctcaagcgcg tcgagcatga gcgtagtcgc cagcgcgccg aggttgaagc cgacggcgtg
      481 ctttacgaaa tcgacctgcc gctggccggc gattttcaaa ttgccaatgc gctggtttcg
      541 gccgggcttg ccatttccac gggaacgccc gtcgccaccg cgattgccgc gctggaaaag
      601 ctgaaaggcg cgccggggcg gctcgatctg atcggcacca ccgccaatgg cgcgccggtc
      661 tatgtcgatt atgcgcataa gccggatgct ttggagaatg tgctgagcgc cgtgcggccc
      721 ttcaccactg gcaaggtcgt cgtggtgttc ggctgcggcg gcgaccgcga caggggcaag
      781 cgaccgatca tgggtgagat cgccacacgg cttgccgatg tcaccatcgt caccgacgac
      841 aatccgcgtt cggaagcgcc ggagacgatc cgcgccgcca tattggccgc tgcccccggc
      901 gccatcgaga tcggcgaccg gcgcaaggcc atccacgaag cggtcgccat gttgcatgcc
      961 ggcgacacgc tgatcgtggc cggcaagggg catgaagagg gccagaccat cggctcggaa
     1021 acgttccact tttccgatca tgaggaagtg cgcgccgcgc tgaaggatcg cgccgcatga
     1081 accggctatg gacctccgag gcgctggttg cggccatgga cggcaggccg atcggctcga
     1141 tgccggaagg cattggcggc atatcgatcg atacgcgcag ccttcagccg ggcgacgctt
     1201 tctttgccat caagggcgag acgatggacg gccacgattt cgccaccgcc gccatcaagg
     1261 cgggggccgg cgttctggtg gtggcggaag gcaagctgcc ggcgctgggg cggctgaccg
     1321 cgccgatgat cgtggtgccg gacgtgcttg aagccttgca gaagctgggc atcgccgcgc
     1381 gcgcgcgttc gcgggcaaag atcatcgcgg tcaccggctc ggtcggcaag accagcacca
     1441 aggaagcgct gcgccacgcc ctttcggctg tcggcaaggt tcatgcttcc gacaaatcgt
     1501 tcaacaatca ctggggcgtg ccgctgacct tggcgcggat gccggacgat tgcgatttcg
     1561 ccgtgttcga gatcggcatg aaccatcccg acgagatccg tccgctggtc aagatggtgc
     1621 ggccgcatat cgccgtcgtc actttgatcg cggcggccca tctcggtttc ttccgtaacc
     1681 tggaagagat cgccaaggcc aaggcggaga tattcgaagg gctggagccg ggtggcacgg
     1741 cgctgctcaa ccgcgacgat ccgcgctcga agctgctgga aaagctggcg gtggccgccg
     1801 gcgtcgagaa catcgccggc ttcggcgaga atgcgcgctc gacctaccgg ttgatcgatt
     1861 gcgcgctgca tgcggatcat tccgtcatcg ccgtcaagat cggcggcgag gagatgatcg
     1921 tacgcatcgg cgcgccggga aggcatatcg cgcagaacgc gctcgcggtg ctgggcgcgg
     1981 cgcatctggc cggcgccgat gtcgcggccg tggcactggc gctcggcagc ctgtcggctg
     2041 aagccgggcg gggcaagcgg catattctgc gccacccgga agggccgttc acgctgatcg
     2101 acgagagtta taacgcaaat ccggcctcga tggcagcggc gctggccttg ctcgacgcca
     2161 cgccggtatc cggccagggc cggcgcatcg ccgttttggg cgacatgctg gaactgggcg
     2221 cgcacggacc caagctgcat gccgggcttg ccgaacagat cgtcggcacc ggcattcaaa
     2281 cggtgttcct cggtgggccg gagatgaagg cgctggccga tgttttgccg ggcgatatcg
     2341 caaccgccta ccgcgaaggc gcggacgaat tgaagcccgt cttgctgggt acggtcgggc
     2401 cgggcgacgt ggtgatggtc aagtcgtcca agagtatcgg cttttcgaag cttgtcgaag
     2461 cattgctgaa gaaatttccg gcgcaggccg aaggctgaag ccggttcggg ggaagacata
     2521 aatgtttacg ttgctcgttg attttgcaga ccggatcacg gtcttcaacg tcttccgcta
     2581 catcaccttc cgcaccggcg gcgcgctgat cacgtcggcg cttatcgtct tcatttttgg
     2641 accgaccatc atcaattcgc tgcggctgcg gcagggcaaa ggccagccta tccgcgccga
     2701 cggaccgcag acccatttca agaaggccgg cacgccgacc atgggcgggc tgatgatcct
     2761 gacgggcatc atcatttcgg cgctgctgtg ggcgaatttg cgcaatgtct atgtctgggt
     2821 ggtgctgctg gtgacggtgg gcttcggcgc catcgggttt tacgacgact atctgaaagt
     2881 caccaagcaa tcgcatctgg gcttttcagg caaggctcgg cttggcctcg aattcctcat
     2941 cgccggcatc gccagttggc tgattatgca tgcggggcag gagccgttct cgtcgtcgct
     3001 gacatttccg ttcgccaagg aatttctcat caacctcggc tggttcttca ttccgttcgg
     3061 cgctttcgtc atggtcggcg cgggcaacgc ggtcaacctg accgatgggc tggacgggct
     3121 ggcgatcgta cccattatga tcgccgccgc atcgtttggg gtgattgcct atctgtcggg
     3181 taacgcggta tttgcagagt atctgcaaat acacttcgtt ccgggcaccg gcgaactggc
     3241 cgtggtgctg ggcgcggtga tcggggccgg cctcggcttc ctgtggttca atgcgccgcc
     3301 ggccgccatc ttcatgggcg acaccggatc gctggcgctg ggcggcctga tcggcacggt
     3361 ggctgtggcg accaagcacg agatcgtgct ggcgattgtc ggcggcctgt tcgtggtcga
     3421 gattttctcg gtcatcatcc aggtcggtta tttcaagatg acgggcaagc gggtgttcct
     3481 gatggcgccc atacatcacc atttcgaaaa gctcggctgg accgagagcc aggtggtcat
     3541 ccgcttctgg atcatcgcgg tcatcctggc gctggtcggc ctctccaccc tcaagctcag
     3601 ataggaacat tgcagtccaa tgatcccggc ggcaacattt gcaggcaagc gcgtcagcct
     3661 cttcggactg ggaggctcgg gcatcgccac ggcgcgcgcc ctgatggagg gcggcgcgga
     3721 tgtgctggcc ttcgacgata atcccggcag cgtcgccaag gcccaggccg aaggcattcc
     3781 gacaggcgat ttgcgcgggg ccgactggtc gcgcttttcg tctttcgtgc tgtcgcccgg
     3841 cgtgccgctg acgcatccca agccgcactg gaccgtggaa ctggcgcgcg gcgccggcgt
     3901 cgaaatcatc ggcgacgtcg aactgtttgc gcgcgagcgc attgcgaggg cgcccgacgc
     3961 gccgttcatc gccatcaccg gcaccaacgg caagtcgacc accacggcct tgacggcgca
     4021 cattctcaaa gccgccgggc gcgatgcgca gatgggcggc aatatcggcc gcgcggtgat
     4081 gacgctcgat ccgccgcaag cgggccgtca ctatgtggtg gaatgctcgt cctaccagat
     4141 cgacctcgcg ccctcgatca acccgaccgc cggcgtgctg ctcaatctga caccggacca
     4201 tctcgatcgt cacggcacca tgcagcacta cgccgcgatc aaggaaaggc tggtggcggg
     4261 cagcgacacg gcgatcatcg gcatcgacga cagctggtgc gcgcagatcg ccgaccggct
     4321 ggagcgcgtc ggcaaggaag tcatccgcat ttccaagcgc ctgccgctga ccgatggtta
     4381 tttcgccgat ggcaccgacc tgatggaagc ggtgcagggc cgctatagcc gcatcggctt
     4441 tctggaaggc atcggttcgc tgcgtggcca gcacaatgcg caaaacgcgc ttgccgccgt
     4501 cgccgcctgc ctgaaagtcg ggctggagct tggcgaaatc cagtcgggac tggaaagctt
     4561 tccggggctg gcgcaccgca tggagcaggt gggccgcaaa ggccatgtgc tgttcgtcaa
     4621 cgattccaag gcgacgaatg cggatgccgc cgcacccgct ttgtccagct ttccgcgcat
     4681 ctactggatt gcgggcggcc tgcccaagga aggtggcatc gagccgctgc gcgggctgtt
     4741 tccgcgcatc gccaaggcct atctgatcgg tgaggcggcg ccggcttttt cagcgaccct
     4801 tggcgagacg gttccttatg aaatatcggg cacgctggcg gcagccgtgg agcatgcggc
     4861 ggccgatgcc gaaaaggaca atagcggcga ggtggtggta ctgttgtcgc ctgcctgcgc
     4921 cagtttcgac cagttcaaga atttcgaact gcgcggcgaa ttcttcaggc aagctgcgcg
     4981 cgctatcgat ggcgtgaagg tgatcggaga gatacgataa tggcacagcg gagccgcctc
     5041 gacaaaagcc cggttgcgac atggtggtgg accatcgacc ggtggttcct tgcggcgttc
     5101 ctgtcgctga tgggcctcgg catcgtcctt tccttcgcgg caagccccgc ggtggctcag
     5161 cgcatcgggc ttgatccgtt ccatttcgcc acgcgccaga tcatcttcac catcccggcg
     5221 ctcggcgtca tgctggcggt gtcgttcctt gaatcgcgcc agatcaggcg catggcgctt
     5281 atcatgctcg ccatcatgct ggtgctgatg gtggtcgtgc tctatgtcgg cgtcgaggtg
     5341 aagggcgcgc ggcgctgggt
//
LOCUS       DASYCS010000263         2395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4707050,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000263 DASYCS010000000
VERSION     DASYCS010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4707050"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..1033)
                     /locus_tag="VGM46_08500"
     CDS             complement(65..1033)
                     /locus_tag="VGM46_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019996585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HEY4192675.1"
                     /translation="MFRSRWFRLAALFAVMLSVFSMAAIDMAEARSGGSFGSRGMRTY
                     QSIPSTPTVPHITGPVQRSMNTPSATNPNGYGSNFGRPGLFGGFGGWLLGGLLFSGLF
                     GMMFGGGFGGFGGIFSLLIQLGIAFFVVRWLIGRFGRTASPGAGYGAGNSYNYAGPGA
                     GPQAGPGFGGFGSAGRQTSDASRRAGNRDEIGIGDADLQVFEQRLKQLQDAYSREDYD
                     ALRRISTPEVMSYLTEELAQAAAQGLRNEVFDVNLLSGDVAEAWREGADEYATVALKY
                     ESRDVMRERATGKLVSGEDRVMERSEVWTFVRHNRGDWLISAIQGA"
     gene            1397..1666
                     /locus_tag="VGM46_08505"
     CDS             1397..1666
                     /locus_tag="VGM46_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192676.1"
                     /translation="MTHQDYSARAELYLGSDRATAFAQGPRAFRTAAHAIRFAIEEAA
                     PVSLRGARLVTNNATFWGDDLVRLHNSPDYPLPRRRAASARKKRN"
     gene            1668..1919
                     /locus_tag="VGM46_08510"
     CDS             1668..1919
                     /locus_tag="VGM46_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192677.1"
                     /translation="MPKFDYAAMAELYPSRRFAKSQTTQYRRFSTAAEAVQYIVEEMP
                     AKWLAGSYLEVNEQRFEGAAIRALYDAEDYPLERETVAA"
     gene            1983..>2395
                     /locus_tag="VGM46_08515"
     CDS             1983..>2395
                     /locus_tag="VGM46_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEY4192678.1"
                     /translation="MTDAPPPFLDVANSVTGRVWVDRLDERETRIAQAIAQRSGLSEI
                     LARILAARGVGIDMADEYLNPTIRGLMPDPSSLADMDRLAARLVQAVTDSEKVALFGD
                     YDVDGASSCALLSRYLTHFGLAPDVYIPDRVFEGYG"
BASE COUNT          417 a          856 c          720 g          402 t
ORIGIN      
        1 tgcggaaccg gtaataacaa aacgggcggc ctctcgaccg cccgctgaac tcatctgatc
       61 gggcttacgc cccctggata gcgctgatta gccagtcgcc gcggttgtgg cgcacaaagg
      121 tccagacctc cgaacgctcc atcacccggt cctcgccgct gaccagcttg cccgttgccc
      181 gttcgcgcat cacgtcgcgg ctctcatatt tcagcgccac cgtggcgtac tcgtcggcgc
      241 cttcgcgcca ggcttcggcc acgtcgccgc tcagcaaatt gacgtcgaac acctcgttgc
      301 ggagaccctg cgccgccgcc tgcgccagct cctcggtgag gtacgacatc acctcgggcg
      361 tcgaaatccg ccgcagcgcg tcatagtctt cgcgcgaata ggcgtcctgc aactgcttca
      421 ggcgctgctc gaacacctgc aggtctgcgt cgccaatgcc gatctcgtcg cgattgccgg
      481 cgcggcgcga ggcgtcggag gtctgccgac cggcgctgcc gaacccgccg aaaccggggc
      541 cggcctgcgg acccgcgccc ggcccggcat aattgtagct attgccggcg ccatagcccg
      601 cgcccgggct ggcggtccgg ccgaagcggc cgatcagcca gcgcaccacg aagaacgcga
      661 tgccgagctg aatcagcaag ctgaagatgc cgccgaagcc accaaagccg ccgccgaaca
      721 tcatgccgaa caggcccgag aacagcaggc cgccgagcag ccagccgccg aagccgccga
      781 acagccccgg ccggccgaaa ttgctgccat agccattggg gttggtggcg ctcggcgtgt
      841 tcatcgagcg ctgcaccggt ccggtgatgt gcggcaccgt cggcgtcgac ggaatgctct
      901 ggtaggtccg cataccgcgg ctgccgaagc tgccgccgct gcgggcctcc gccatgtcga
      961 tggccgccat gctgaacacc gacagcatca cggcgaacag ggcggcgagg cgaaaccagc
     1021 gagaacggaa cattgtggtc ccccacgaaa acgatgcgct gtatatggga gccgcgagac
     1081 cgagttttag accctcgcaa ggcccggccg tccgattttg cccagctccc gaaccgcgcc
     1141 gttcactacg ccggaccgcg cggccttgtt attgccgcag caaacgacta tctacagagc
     1201 atacgttttc agccagtttg gcttgctttc gccggtgcac cggcgtcccg ctatccccaa
     1261 agtgcgaacg ttacgtcttc cgatcgccgc gtctgcggca ggaagcggcg aaacccttgc
     1321 gcagttcggc gcggtttcga ttttccgaca attcaacgaa gcggcctgtg gcggtgccac
     1381 cggcctttag agacacatga cacatcaaga ctactcggct cgtgccgagc tttatctcgg
     1441 cagcgaccgc gcgactgctt tcgcgcaagg tccgcgcgcc tttcgtactg ccgcccacgc
     1501 tatccgtttc gccatcgagg aagcggcccc agtcagcctg cgcggtgcgc ggctcgtcac
     1561 caacaacgcg acgttctggg gcgacgacct cgtccggttg cacaatagtc ccgactaccc
     1621 cctgccgcgc cgccgcgccg cgtccgctcg aaagaagagg aattaagatg cccaagttcg
     1681 attatgccgc catggccgag ctctacccga gccgccggtt cgccaagtcg cagaccacgc
     1741 agtatcgccg gttttcgacc gcggccgagg ccgtgcagta catcgtcgag gaaatgccgg
     1801 ccaaatggct ggcgggctct tatctcgagg tgaacgagca gcgcttcgaa ggcgccgcta
     1861 tccgcgctct ctacgatgcg gaagactatc cgctcgagcg cgagaccgtc gccgcctgag
     1921 cgaacgaaaa tgctgcgtcg ccggtctcga cggcgcagcc cctttcggcc taactccccg
     1981 ccatgaccga tgcgccgccg ccgttcctcg atgtagcgaa ctccgtcacc ggtcgcgtct
     2041 gggtcgaccg cctcgacgag cgcgagaccc gtattgccca ggccatcgcg caacgctcgg
     2101 gcctgagcga aatcctcgcc cgcatcctcg cggcgcgcgg cgtcggcatc gacatggccg
     2161 acgaatatct caaccccacc atccgcggcc tgatgcccga tccgtcctcg cttgccgaca
     2221 tggacaggct ggcggcgcgg ctggtgcagg ccgtcaccga tagcgagaag gtggcgctgt
     2281 tcggcgacta cgacgtcgat ggcgcctcgt cctgcgccct gctgtcgaga tatctcaccc
     2341 atttcggcct cgcgccggac gtctacatcc ccgatcgcgt cttcgagggc tatgg
//
LOCUS       DASYCS010000264         3876 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4707170,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000264 DASYCS010000000
VERSION     DASYCS010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4707170"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="VGM46_08520"
     CDS             complement(<1..628)
                     /locus_tag="VGM46_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TadG family pilus assembly protein"
                     /protein_id="HEY4192679.1"
                     /translation="MQLSGGRLPKLLKRLVTDRRANFAVMTALSAPFAIALCAVAVDQ
                     GSLFTERRSAQALVDIAAITAAANLSKAETAVLLTLADNGLPAAEIDNAGMRRPTPAK
                     PTVSVVQGRYSATAALGARFQAGQQPYNAVRVSLREIGTLYFGSALMAPPVIGTTAVA
                     STSATAAFSVGSGLIEVDTTKSAILNAVLGGLLGTSLSLKVMDYEALAR"
     gene            complement(628..1101)
                     /locus_tag="VGM46_08525"
     CDS             complement(628..1101)
                     /locus_tag="VGM46_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEY4192680.1"
                     /translation="MTRAIHSSSDNRFRASRFWASHFGADRTGTSAIEFAMLAPVFLL
                     FLFGMIAYGIYFGASHSLQQITADAARTAIAGQSATERSAIVADFIANNAAGYPFIDR
                     AKLTVSAQDSGSDGNQFNVQVSYDARNLPIWNLLPDLPLPGTTIVFRSTIRVGGV"
     gene            complement(1244..1320)
                     /locus_tag="VGM46_08530"
     tRNA            complement(1244..1320)
                     /locus_tag="VGM46_08530"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1284..1286),aa:Pro,seq:cgg)
     gene            1521..3041
                     /locus_tag="VGM46_08535"
     CDS             1521..3041
                     /locus_tag="VGM46_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006330896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEY4192681.1"
                     /translation="MTLEIVGEARPNTFEFETQALVKASGFREYDARWWFGHPASAVP
                     PELNLIGVQALGMGLGTLIRRRGVGPDIVTGHDFRAYSLGIKLALVSGLMAAGARVKD
                     VGLALSPMAYFAQFALDTPSVAMVTASHNENGWTGVKMGAQRPLTFGPEEMTALKEIV
                     LAGDFDLVGGGSYDFIPGFRETYLADLTAGKKIARKLKVVVACGNGTAGAFAPQALAK
                     IGCEVIPLDTELDHSFPRYNPNPEDMKMLHAIRDKVLETGADVGLGFDGDGDRCGVVD
                     NEGNEIFADKVGVMLARDISARHPGSTFVVDVKSTGLFNTDPVLRANGAKTDYWKTGH
                     SYIKRRVAELGAIAGFEKSGHFFFNPPIGRGYDDGLVTAIAVCEMLDRNPQAGMADLY
                     RALPLTFGTPTMSPHCDDALKYGVVERVVADFEAMKRQDAIFAGQKIAALVTVNGIRV
                     VAEDGTWGLVRASSNKPELVVVVESPVSSERRRQMFEAVDEVLRRSPEVGAYNQTF"
     gene            3053..>3876
                     /locus_tag="VGM46_08540"
     CDS             3053..>3876
                     /locus_tag="VGM46_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010914983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEY4192682.1"
                     /translation="MNERIASFVMSGGVGSRLWPLSREDNPKQFHDLSGDGSMLVKTL
                     RRLTARPEGETPVFLIAAERHADRIRTDLAGMDLSGGGALFEPAGRNTAAAVALATLR
                     TLSQFGDSLVLVVPSDHEISTVNQFWQSVEDGVAAANEGRLVVFGIKPTQPETGYGYI
                     EAGAESHGVFDVSRFVEKPDLATAQSYLAAGNFYWNTGIFLFRASAMRDAFAAFQPEI
                     WQATEAAYNAATSDLSGLYMPLELYSAIPSISIDYAIMERAKRIAMVPAGFRWNDLG"
BASE COUNT          713 a         1190 c         1242 g          731 t
ORIGIN      
        1 cgcgcgcaag cgcttcgtag tccatgactt tcagcgacag gctggtgccg agcaggccgc
       61 cgagcaccgc gttgaggata gccgatttcg tcgtgtccac ctcgatgagg ccggaaccga
      121 cggaaaatgc agccgtcgcc gatgtgctgg ccaccgccgt ggtgccgatg acgggcggcg
      181 ccatcaacgc gctgccgaaa tagagcgtgc cgatctcgcg cagcgaaacc cgcacggcat
      241 tatagggctg ctgccctgcc tggaaacggg cgcccagcgc agccgtcgcg ctatagcggc
      301 cctgaaccac ggaaacggtc ggcttcgcgg gcgttggcct gcgcattccc gcattgtcga
      361 tttcagcggc tggcaaccca ttatcggcca gcgtcagcag aaccgcggtt tcggctttgg
      421 agagattggc cgccgcggtg atggcggcaa tatcgaccag cgcctgtgcc gaccgccgct
      481 cggtgaaaag cgatccctga tcgacggcca cggcgcacag ggctatcgca aagggtgcgc
      541 tgagcgccgt catgacggca aaattcgccc gcctgtcggt gacaagcctt ttcaggagtt
      601 taggtagcct gccgccggaa agttgcatca gacgcccccg acccggatcg tcgagcgaaa
      661 cacgatggtg gtgcctggaa gcggaaggtc gggcagcagg ttccagatcg gcaggttgcg
      721 cgcgtcgtag ctgacctgga cgttgaactg gttgccgtcg ctgccgctgt cttgcgcgct
      781 gacggttagc ttggcgcggt cgatgaacgg gtagccggcg gcgttgttgg cgatgaagtc
      841 ggcgacgatg gcgcttcgct cggtcgcgct ctggccggca atcgcggtgc gcgccgcatc
      901 ggcggtgatc tgttgcagcg agtggctggc gccgaaatag atgccgtagg cgatcatccc
      961 gaacaggaac agcaggaaaa caggggccag catggcgaat tcaattgccg atgtgccggt
     1021 tcgatccgcc ccgaaatggc tggcccagaa gcggctggcc cggaaacgat tatcggaact
     1081 ggaatggatc gcccttgtca ttcccggaga atgccaagcc cggatgcagg agtgagaaat
     1141 cttttatgca acctggaatt gataaaggtt gcataatgca actccgatga gttgaaaccc
     1201 atcgcccgca gcaagccgaa ggctcggcca ttttgggata gactggtcgg agtggcagga
     1261 tttgaacctg cgaccccgtc gtcccgaacg acgtgcgcta ccagactgcg ctacactccg
     1321 cgaccagacg ggcggcttat atccggcacg ctccgatcct gcaagcgtca aaggaagtat
     1381 tgatatggca atttgtcggc ttgcagccgg aagtggcgct tctttaagtt tcccgtaggc
     1441 aaacgcggct agaaggtttg tatggcgcta taatttttcg gcgcccgtgc atcgtatgca
     1501 gcgttttgga gcttattggc gtgacccttg agatcgttgg cgaagcccgg ccgaacacgt
     1561 tcgagttcga aacgcaggca ctggtcaagg cgtcgggctt tcgcgaatat gacgcgcgct
     1621 ggtggttcgg gcatccggcc tcggcggttc cgccggaact caacctgatc ggcgtgcagg
     1681 cgctcggcat gggacttggc acgctgataa ggcggcgcgg cgtcggcccc gatatcgtca
     1741 ccgggcatga tttccgcgct tattcgctag gcatcaagct ggcgctggtg tcgggactga
     1801 tggcggcggg cgcgcgggtg aaggatgttg gcctggcgct gtcgccaatg gcctatttcg
     1861 cgcaattcgc gctcgatacg ccctcggtcg ccatggtcac cgcttcgcat aacgagaatg
     1921 gctggaccgg cgtcaagatg ggcgcgcaac ggccgctcac cttcggcccc gaggaaatga
     1981 ccgcgctgaa ggaaatcgtg ctggccggcg atttcgacct cgtcggtggc ggaagctacg
     2041 atttcatccc cggtttccgc gaaacctatc tggccgacct caccgccggc aagaagatcg
     2101 cccgcaaact caaggtggtc gtggcctgcg gcaacggcac ggccggcgcg ttcgcgccgc
     2161 aagcgctggc gaaaatcggt tgcgaagtca tcccgctcga taccgaactc gaccacagct
     2221 tcccgcgcta caacccgaac ccggaggaca tgaagatgct tcatgccatc cgcgacaagg
     2281 ttctggagac gggcgccgat gtcgggcttg gcttcgacgg cgacggcgac cgctgcggcg
     2341 tggtcgacaa tgaaggaaac gagatttttg ccgacaaggt cggcgtcatg ctggcgcggg
     2401 acatttccgc gcgtcatcca ggctctacct tcgttgtcga tgtgaagtcg accggccttt
     2461 tcaacactga tccggtgctg cgcgccaacg gcgcgaaaac cgactactgg aagactggcc
     2521 attcctacat caagcgccgc gtcgccgagc ttggcgctat cgccggcttc gagaaatccg
     2581 gccatttctt cttcaacccg ccaatcgggc gcggctatga tgacgggctg gtgacggcca
     2641 tcgcggtctg cgaaatgctc gaccgcaacc cgcaggccgg catggccgat ctttaccgcg
     2701 cgctgccgct gacttttggc acgccgacca tgtcgccgca ttgcgacgac gccctgaaat
     2761 atggcgtggt cgagcgtgtc gtcgccgatt tcgaggcgat gaagcggcag gatgccattt
     2821 tcgccgggca gaagatcgca gcgctcgtca ccgtcaacgg catccgcgtc gtcgccgaag
     2881 acggcacatg ggggctggtg cgcgcctcgt ccaacaagcc tgagctggtg gtggtggtgg
     2941 aaagccccgt ctcgtccgag cgccgccggc agatgttcga ggcggtcgat gaagtgcttc
     3001 gccgcagccc cgaggtcggc gcctacaacc agacattctg accgagggat cgatgaacga
     3061 gcgaattgcc agttttgtca tgagcggcgg cgtcggctcg cggctgtggc cgctgtcgcg
     3121 cgaggataat cccaaacagt tccacgatct ttccggcgac ggttcgatgc tggtcaagac
     3181 gctgcgccgc ctgacggcgc ggcccgaagg tgaaacgccg gttttcctga tcgccgccga
     3241 gcgtcacgcc gaccgcatcc gcaccgacct tgccggcatg gacctttccg gcggcggcgc
     3301 cttgttcgag ccggccgggc gcaacacggc cgccgccgtc gcgctggcca ccttgcgcac
     3361 gctttcccag ttcggtgaca gcctggtgct ggtggtgcct tccgaccacg aaatctcgac
     3421 ggtaaaccag ttctggcaaa gcgtggagga cggcgtcgcc gccgccaatg aaggccggct
     3481 ggtggtgttc ggcatcaagc cgacccagcc ggaaaccggc tatggctata tcgaggccgg
     3541 ggccgagagc cacggcgtgt tcgatgtttc ccgtttcgtg gaaaagccgg atctggcaac
     3601 cgcgcaaagc tatctcgcgg ctggaaattt ctactggaat accggcattt tcctgtttcg
     3661 cgccagcgcc atgcgcgacg ccttcgccgc attccagccg gaaatatggc aagccaccga
     3721 ggccgcctat aatgccgcca ccagcgatct ttccggcctt tatatgccgc tcgaactcta
     3781 ttcggccatt ccatccattt ccatcgacta tgcgatcatg gagcgcgcca aacgcatcgc
     3841 catggtgccg gccggctttc ggtggaacga tctcgg
//
LOCUS       DASYCS010000265         6797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_647739,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000265 DASYCS010000000
VERSION     DASYCS010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 6797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6797
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647739"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..1107)
                     /locus_tag="VGM46_08545"
     CDS             complement(25..1107)
                     /locus_tag="VGM46_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY4192683.1"
                     /translation="MRKLTRRLTLTLALAATFAASAAAFAVAEDKSLIIFDWAGYEDP
                     SFHPKYVEKNGDSPTFAFFGDEDEAFEKLRSGFKADLGHPCSQSVEKWREAGLLQPLD
                     TSKITGWNDLLPGLMSMKNLATTEDGKAWFMPWDWGNTQLTYNSDHVDAKDVQSLKAF
                     ADPKFKDRVSIGDNVDDAYALASLAIGLKDWNKMTDDQFKQASDFLRQVHKNVRLYWT
                     DTTDIVQALSGGEIDLAWAWNDATVQSVAAGAPIKSKKDTDEGISTWVCGYVLFKDAP
                     GNIDKAYDYLNAVNDPEVAKTLVGDWGYGQGNAKGMEGVDPAVLKAKGYDDVQKFVDK
                     TLFQSPIPSTLKLKMIAEFEKIKAGY"
     gene            complement(1135..1827)
                     /gene="betI"
                     /locus_tag="VGM46_08550"
     CDS             complement(1135..1827)
                     /gene="betI"
                     /locus_tag="VGM46_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HEY4192684.1"
                     /translation="MTMQEEQLLEKAKKAEPEKRGRKASKEVRRQQLIEATIDSLAKR
                     GYSDTTMADVADGAGLSRGIVNFHFESKEKLLVATLQHMYDEYAAHWRAALEKAGDDP
                     ASKLQALVAADFDRAICNKRKLAAWCAFWGEAKSRPTYQALSGARDQAYQGTVVSLCG
                     LLKQDGGYDCDPESIALALSAMLEGLWLRLMMGTQDVSRETALNGANAFLASAFPKHY
                     PRERTGRSAART"
     gene            2238..3857
                     /locus_tag="VGM46_08555"
     CDS             2238..3857
                     /locus_tag="VGM46_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY4192685.1"
                     /translation="MKKWDAIVIGGGHNGLVNACYLQRAGLDVLVVEKNDWVGGAATS
                     RSHTSGFLYSNCSYVCSLFRPEIMRDLELPRFGLQVISYEGGAVLTRDGDYLANYRDH
                     DAHRREFARFSKRDAEAYDRYARDVTRQCRFIQPLLMRTAPDPTSFKPRDIGELLYLG
                     KKFGGLSSQEMALTLRFWTMSISEFLDEYFETDVIKANFAISGIIGTALGPMSPGTAY
                     VLLHHYMGEVDGSVGAWGYARGGMGAVTQALAASFRASGGTIRTGAEIDHVLVSRGKA
                     KGVVLAGGEEIPAKLVVSNADVKRTFLKLVEEKELPDIFLRRVRNFKIRGSSGKVNIA
                     LDSMPEFPALPKDSPCTRGDLHFTDSVERMERAYDDWKAGTWSADPMLDMMIPTTLDP
                     TMAPPGKHFMSCFVQYAPPKIAGREWTDADRDGFAESVVSQIAEYSPGFRDRIIHMEV
                     RTPREIEAEVGLTEGNIFQGELTFDQLLFNRPVPGYAQYRSPVGGLYMCGSSTHPGGG
                     VMGAPGRNAAAEILRDLAKPTHHMSPAHDVI"
     gene            3844..5424
                     /locus_tag="VGM46_08560"
     CDS             3844..5424
                     /locus_tag="VGM46_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY4192686.1"
                     /translation="MTSFDIPKWDVAIIGGGHNGLVCSATLARSGRKVLVLEAAGEVG
                     GAARTEEFAPGFRVSSVAHLLNRLHPDVVRTLELEKHGLEFSRQGFAPSVALSKDGKP
                     LMLHGAYGEALSGASASEQVAWKELRAQLLRYAGVLKPLLAHRPPALSGLSLGEKMVL
                     GKTGLALKRLGKEDMRDFLRVLLMNVADLLDEQLSDDRLKGLLAFDAVLGAHLGPRSP
                     TSLLGLYYRLTGETGGDTGAQMLPKGGMGAVVDALRASAEKAGVSIRTAAPVGELIVE
                     KGRAVGVLTEKGEEIRARTVVSAINPATTFLDLVGATTLDTGFVRKVTNIRMKGDAAK
                     LHLALDRAPEFLGVDAVGHMGRLVIAPSTDHVERAFNPSKYGEFSPEPVMEITLPSLS
                     DPSLAPDGACVLSAVVQYAPYALREGWEAGKPKFMAAIMAQLEAYAPGIGKTVRHAEL
                     LTPADIEARYRMPGGHWHHGELQADQMLVSRPVSGWSGYDTPIDGLYLAGAGSHPGGG
                     VSGAPGLNAARRVIAVEK"
     gene            5494..6657
                     /locus_tag="VGM46_08565"
     CDS             5494..6657
                     /locus_tag="VGM46_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HEY4192687.1"
                     /translation="MGTPFQPRIDALAKTNDWYDWAGYRAPGSLWDEELEYFAIRSQA
                     ALFDISPMTKYRIEGPDAEAFCNKVTLRDVAKLKPGRVHYTAWCDDEGHVLDDGTLFR
                     LSPTRFRLCSQERHLPWLLDSGLGFDVTVEEETEAVAGLALQGPTSFAVLREAGFAGV
                     ERLKVFDLAEFPHEGGAVTISRTGFTGDLGYELFVPAALALSLWDRLMEAGQSRGIRA
                     IGYTALNRARLEAGLIVANGDFIAADHAIRADRLRMPDEIGLGFMVDAGKGHFNGRRA
                     ILEARAKKRLRHVLVGLEIEGNIPAEHAIVYYRKSQEAGLISAAMWSPMAKRNIAIAS
                     LALPYGDTVVDDLWVEIYAMRELQYQKMMKRAKVVQRPFIKLDRRTANPPADF"
BASE COUNT         1166 a         2045 c         2254 g         1332 t
ORIGIN      
        1 cgacgatccg gcaggtctcg ttcgtcaata gccggctttg atcttctcga actcggcgat
       61 catcttcagc ttgagcgtcg atgggatcgg cgactggaac agcgtcttgt cgacgaattt
      121 ctgcacgtcg tcatagccct tcgccttcaa caccgccgga tcgacgcctt ccatgccctt
      181 ggcgttgccc tggccatagc cccagtcgcc aaccagcgtc ttggccactt ccggatcgtt
      241 gacggcgttc agatagtcat aggccttgtc gatgttgccc ggcgcgtcct tgaacagcac
      301 gtagccgcag acccaggtgg aaatgccctc atcggtatcc ttcttggact tgatcggcgc
      361 gccggcggcc accgactgca ccgtggcgtc gttccaggcc caggcaaggt cgatttcgcc
      421 gcccgacagc gcctgcacga tgtcggtggt gtcggtccag tagaggcgca cgttcttgtg
      481 gacctggcgc aggaagtcgg acgcctgctt gaactggtca tccgtcatct tgttccagtc
      541 cttcaagccg atggcgaggc tggccagcgc ataggcgtcg tcgacattgt cgccgatgga
      601 aacgcggtcc ttgaatttcg gatcggcaaa ggccttcagc gactgcacgt cctttgcatc
      661 gacgtggtcg gaattatagg tgagctgggt gttgccccag tcccagggca tgaaccaggc
      721 cttgccatcc tcggtggtgg ccaggttctt catcgacatc aggccgggca gcaggtcgtt
      781 ccagccggtg atcttcgagg tgtcgagcgg ttgcagcagg ccggcctcgc gccacttttc
      841 gacgctttgc gagcaggggt ggccaagatc ggccttgaag ccggagcgca gtttctcgaa
      901 ggcttcatcc tcgtcgccga aaaaggcgaa ggtgggcgaa tcgccgttct tctcgacata
      961 tttcgggtgg aagctggggt cttcgtagcc tgcccaatcg aagatgatca ggctcttgtc
     1021 ttcggcaacg gcgaatgccg cggccgatgc ggcgaatgtg gcggcaagcg ccagcgtcag
     1081 tgtcaggcgt cgggtaagct tcctcatttt ttcgtcctcc cattatcgtc gatgtcaggt
     1141 tctggctgcg cttcgccccg ttcgttcgcg cgggtagtgt tttggaaagg cggaagccag
     1201 aaaggcgttg gcgccgttga gcgcggtttc gcgggaaacg tcctgcgttc ccatcatcag
     1261 ccgcagccac aggccttcca gcatggcgct aagcgcaagc gcgatggatt ccgggtcgca
     1321 atcatagccg ccatcttgtt tcagcaggcc gcacaggctg acaacggtgc cctgataggc
     1381 ctggtcgcgc gcgccgctca aagcctgata ggtcggccgc gacttggcct cgccccagaa
     1441 ggcgcaccag gcggcaagct tgcgcttgtt gcagatggcg cggtcgaaat cggcggcgac
     1501 cagcgcctgc aacttcgagg cggggtcgtc gccggccttt tccagtgcgg cacgccaatg
     1561 ggcggcatat tcgtcgtaca tatgttgcag ggtggcgacg agcagctttt ccttgctctc
     1621 gaaatggaaa ttgacgatgc cgcgcgacag gcccgcgccg tcggcgacat cggccatggt
     1681 ggtgtcggaa taaccgcgct tggccagcga atcgatcgtc gcctcgatca gttgctgccg
     1741 ccggacctcc ttcgaggcct tgcggccgcg cttttccggt tccgccttct tcgccttttc
     1801 cagcaactgc tcttcctgca tcgtcatcgg tgtaccgtct ccaacatcac cggcgtgcga
     1861 acttgccggg tttcagcatc ggcagatgag ccggacggcg ctcgccgctg tcaagcattg
     1921 tctgcgtcgt tggatgggtg aaggtcgtgc ccgaaggccg ggcgcgacag ggtaggcccg
     1981 cctcggtggc agcgtcgtcg tttccagcgt ttgcgtttgc ggccatatgg ctgctgcttc
     2041 ctggtcaggc cccgaggcga aatcttgaag caaaacctat cgcatcaaca ggaaaacgca
     2101 atatcgtttg ttgaacattc attcaataca gctggacagg atgccagaag tcgtgcgaaa
     2161 tcccgcttgg caggcggata gttcacgcga aaatgacagg cgcggtcctc ttaccgcctg
     2221 cggccatggg agcttgaatg aagaagtggg acgcaatcgt cattggcggc ggccataacg
     2281 ggctggtcaa tgcctgctat ttgcagcgtg ccgggcttga cgtgctggtg gtggaaaaga
     2341 acgactgggt tggcggcgcc gccaccagcc gctcgcacac gtccggcttc ctctactcca
     2401 actgctccta tgtctgctcg ctgtttcgcc cggaaatcat gcgcgacctt gaattgccgc
     2461 gcttcggctt gcaggtgatt tcctatgagg gtggcgcggt gctgacgcgg gacggcgatt
     2521 atctggccaa ctaccgcgac cacgatgcgc accggcgcga gttcgcgcgt ttttccaaac
     2581 gcgacgccga ggcttatgac cgctatgccc gcgacgtgac gcggcaatgc cgtttcatcc
     2641 agccgctttt gatgcgcacg gcgccggacc cgaccagctt taagccgcgc gacatcggcg
     2701 aattgcttta tctcggcaag aagttcggtg gcctgagttc acaggaaatg gcgctgacgc
     2761 tgcgcttctg gaccatgtcg atttccgaat tcctcgacga atatttcgaa accgacgtca
     2821 tcaaggcgaa cttcgccatt tccggcatca tcggcacggc gctcgggcca atgtccccgg
     2881 gcaccgccta tgtgctgctg caccattata tgggcgaggt agacggctcc gtcggcgcat
     2941 ggggctatgc gcgcggcggc atgggcgcgg tcacccaggc gctggcggct tcgttccggg
     3001 cgtcgggcgg caccatccgc acaggggcgg agatcgacca tgtgctggtc agccgcggca
     3061 aggccaaggg cgtggtgctg gccggcggcg aggaaatccc ggccaagctg gtggtttcga
     3121 acgccgatgt gaagcgcacc ttcctcaagt tggtcgagga aaaggaactg cccgacattt
     3181 tcctgcgccg ggtcaggaac ttcaagatca gggggtcgtc gggcaaggtc aacatcgcgc
     3241 tggactcgat gccggaattt ccggcgctgc cgaaggattc gccctgcacg cgcggcgacc
     3301 tccacttcac cgattcggtg gagcgcatgg agcgcgccta tgacgactgg aaggctggaa
     3361 cctggtcggc cgacccaatg ctcgacatga tgatcccgac cacgctcgac ccgaccatgg
     3421 cgccgccggg aaaacatttt atgagctgct tcgtgcaata tgcgccgccg aagatcgccg
     3481 ggcgtgaatg gacagatgcc gaccgcgacg gttttgcgga gagcgtcgtt tcgcagatcg
     3541 ccgaatattc gcccggcttc cgcgaccgca tcatccacat ggaagtgcgc acgccgcgcg
     3601 agatcgaggc cgaggtcggg ctgacggaag gcaacatctt ccagggcgaa ctgactttcg
     3661 accagttgct gttcaaccgt ccggtgccgg gctatgcgca atatcgctcg ccggtgggcg
     3721 ggctttacat gtgcggctcg tccacccatc cgggcggcgg cgtcatgggc gcgccgggcc
     3781 gcaacgcggc ggctgaaatc ctgcgcgacc ttgccaagcc aacccaccat atgagcccgg
     3841 cccatgacgt catttgatat acccaaatgg gacgtggcga tcatcggcgg cggccataac
     3901 ggcctcgtct gctccgccac gctggcgcgg agcgggcgca aggtgctggt gctggaagcg
     3961 gcaggcgagg ttggcggcgc ggcgcgcacc gaagaattcg cgcccggctt tcgagtttcc
     4021 tccgttgctc atctgctgaa ccgcctgcat cccgatgtgg tgcgaacgct cgaactggaa
     4081 aagcatgggc tggaattttc gaggcagggt ttcgcgccct ccgttgcgct ttcaaaagac
     4141 ggcaagccgc tgatgctgca cggcgcatac ggcgaagcac tctccggcgc gagcgcttcg
     4201 gagcaggtgg cgtggaagga actgcgcgcg caattgctgc gctatgccgg cgtgttgaag
     4261 ccgctgctgg cgcaccgccc gcccgcgctt tccggccttt cgcttggcga aaagatggtg
     4321 ctgggcaaga ccgggcttgc gctgaaaagg ctgggcaagg aagacatgcg cgacttcctg
     4381 cgcgtgctgt tgatgaatgt cgccgacctg ctcgacgagc agctttcgga tgaccggctg
     4441 aaggggctgc tggccttcga cgcggtgctg ggcgcgcatc tggggccgcg ttcgccgacg
     4501 tcgctgcttg ggctgtatta ccggctgacc ggcgaaacgg gcggcgacac aggcgcgcag
     4561 atgttgccca agggcggcat gggcgcggtg gtcgatgcgc tgcgcgcctc ggcggaaaag
     4621 gccggcgttt cgatccggac ggcggctccg gtcggcgagc tcatcgtgga gaaaggccgg
     4681 gcggttggcg tgctgaccga aaagggcgag gaaatccgtg cacgcacggt cgtttccgcc
     4741 atcaatccgg caacgacttt tctcgatctc gtcggggcga caacgttgga caccggcttc
     4801 gtgcgcaagg tgacgaatat tcgcatgaag ggtgacgccg ccaagcttca tctggcgctg
     4861 gacagggcgc cggagttctt gggcgtcgat gccgtgggcc acatggggcg gctggtgatt
     4921 gcgccatcga ccgatcatgt cgagcgcgcc ttcaacccat cgaagtatgg cgagttttcg
     4981 cccgaaccgg tgatggagat cacactgccg tcgctgtccg atccatcgct tgcgccggac
     5041 ggggcctgcg tgctttcggc ggtggtgcaa tatgcgccct atgcgttgag ggaaggctgg
     5101 gaggctggca agccgaaatt catggcggcg atcatggccc agctggaagc ctatgcgccg
     5161 ggcatcggca agacggtgcg ccatgccgaa ttgttgacgc cggccgatat cgaggcgcgc
     5221 taccggatgc ccggcggcca ctggcaccac ggcgaattgc aggccgacca gatgctggtt
     5281 tcacgcccgg tatccggctg gtcgggctac gatacgccga tcgacgggct ttatctggcc
     5341 ggcgcaggct cgcatccggg cggcggcgtt tccggcgcgc cggggctgaa cgccgcgcgg
     5401 cgggtgatag cggtggagaa gtaagtcgtg gcaaaggcat cgtcagcgtc catcgcccgc
     5461 attgccgcgc agcaccattt ccgcgcgctg cgcatgggca cgccgttcca gccgcgcatc
     5521 gatgcgctgg ccaagaccaa tgactggtac gactgggcgg gctaccgtgc gcccggttcg
     5581 ctgtgggacg aggaactgga atatttcgcc attcgcagcc aggcggcgct gttcgacatt
     5641 tcaccgatga ccaaataccg gatcgagggt cccgatgccg aggccttctg caacaaggtg
     5701 acgctgcgcg acgtggcgaa gctgaagccg ggccgggtcc actatacggc gtggtgcgac
     5761 gatgaaggcc atgtgctgga cgacggcacg ctgttccggc tttcgccgac ccgcttccgg
     5821 ctgtgctcgc aggagcggca tttgccgtgg ctgttggatt cggggcttgg tttcgacgtt
     5881 accgtcgagg aggaaacgga agcggtggcg ggccttgcct tgcaggggcc gacctcgttt
     5941 gccgtgctgc gcgaggcggg ctttgccggc gtggaaaggc tcaaggtttt cgaccttgcc
     6001 gaatttccgc acgaaggcgg ggcggtcacc atttcccgca ccggatttac cggcgatctc
     6061 ggctatgaac tgttcgtgcc ggcggcgctt gcactgtcgc tgtgggaccg gctgatggag
     6121 gccgggcaat cgcgcggcat ccgcgccatc ggttatacgg cgctgaaccg ggcgcggctg
     6181 gaagcggggc tgatcgttgc caatggcgat ttcatcgccg ccgatcacgc catccgtgcc
     6241 gaccggctgc gcatgcccga cgagatcggg ctgggcttca tggtcgatgc cgggaagggc
     6301 catttcaacg gccgccgcgc cattctggaa gcgcgggcga agaaacgcct gcggcatgtg
     6361 ctggtcgggc tggagatcga gggcaatatt ccggccgaac acgccatcgt ctattaccgg
     6421 aaatcccagg aagccgggct gataagtgct gccatgtggt cgccgatggc caagcgcaac
     6481 attgcgatcg cctcgctggc gctgccttat ggcgataccg tggtcgatga tctgtgggtc
     6541 gagatctacg cgatgcgcga gttgcagtac cagaagatga tgaagcgcgc gaaggtggtg
     6601 caaaggcctt tcatcaagct cgaccgccgc accgccaatc cgccggcgga tttttgaacc
     6661 atggccggga aagcggatga cgaggcagcc gagcagtggg cgctggtcaa cacgccgctt
     6721 ggcgaggaat ggtcgggccg cacgcgttat gcagcggcga tgttttttca caagcgcggc
     6781 gagatcggcg acggcgc
//
LOCUS       DASYCS010000266         3628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4848901,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000266 DASYCS010000000
VERSION     DASYCS010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4848901"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1207
                     /locus_tag="VGM46_08570"
     CDS             <1..1207
                     /locus_tag="VGM46_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013850606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY4192688.1"
                     /translation="PHLDFAGEIRAATNFPTFHAAKIQDVATARHAIASGKLDMVGMT
                     RAHMTDPHIVRKITQGREHDIRPCVGATYCLDRIYQGGAAYCIHNPATGRETEIPHDL
                     PKAARRRKFVVVGAGPAGLEAARVAAERGHEVVVFEAASHAGGQIRLTAQSRRRREMI
                     GIIDWRVAQCTAMGVKINYSTWADAATVQRENPDVVILATGGIPHTEVLSAGNDLVVS
                     SWDIISGDVKPGTNVLVFDDAGDHSGLQAAEVIAASGGKVEVMTPDRSFAPEVMAMNL
                     VPYMRSLQKLDATFTVTFRLEAVERQGNGLVAIVGSDYGGVRKERRVDQVVVNHGTVP
                     LDELYFELKPQSRNLGVLDHGDFIDGRPQTSAGNPQGTFQLFRIGDAVASRNTHAAIY
                     DALRLVKDL"
     gene            complement(1234..>3628)
                     /locus_tag="VGM46_08575"
     CDS             complement(1234..>3628)
                     /locus_tag="VGM46_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR02302 family protein"
                     /protein_id="HEY4192689.1"
                     /translation="LRFFRLPKPGEVDRRIEAANALPHSPVQVQSDRPSGVETGFSQA
                     LWREHQKRMAATLGHLGADLPRTHVPERDPWALRALAALALAVAFAFSFGSLGGSVGD
                     GFRPSATRDATPPRVDAWVTPPVYTGKPPVFLAAGAADSAAISIPVGSDLSLRVTGGS
                     GDETLAYADAGGNSRELDPSATPTAGKSAPAQATPADQASDNVRQFAARLKANGILTL
                     KSGNSELGRWSFTVTPDKPPQIRFVGESKQAANGAIELGYQIDDDYGAASAKAVFELA
                     QKPAANAHPLYGPPDMPLALPRRGGKANAARTTKDLTEHVWAGAPVTLKLVATDDAGQ
                     MATSETRTLTMPQRPFTNPLALAVIEQRRLLALDANAKPHVLELMDAITLRPQDTFDT
                     MAHYLAIMSARTRLKLADSDDQLRDTVAYLWDIALGIEEGDLSAAEKRLRQAQQALQD
                     ALKNGAGDEEIDKAMKELRQAMNEFLKEFAQRQQNRKSAQMPQDGKEIRQSDLQKMMD
                     QIENLAKSGNRDKAQELLSQLDNMMNNLQAGRQQQQGQQDSEMRRQMDKLGEIMRRQQ
                     EMMNDTFRTDQAQRGQQRREQGEENPFGEFDDQGQPPQGESRQDQPRQGRNGEQQEGK
                     SPEGKPMTPEEFADALKQLQEGQSKLQGDLEKLKKGLEGMGLEPNKGFGQAGKSMGSA
                     EQALGEGEGENAVGHQGKALEELRKGAKDMMKQMQAMQGDQGGSRQGGGRRDADRDPL
                     GRPRATQGPDFGDTVKVPDEIDVQRARQILEAIRKRLGNALSPDIERSYLERLLQLK"
BASE COUNT          541 a         1194 c         1202 g          691 t
ORIGIN      
        1 gccgcatctg gatttcgccg gtgaaatccg cgcggccacc aactttccga ccttccatgc
       61 cgccaagatc caggatgtgg cgacggcgcg ccatgccatc gcctcaggca agctcgacat
      121 ggtgggcatg acgcgcgcgc acatgaccga cccgcatatc gtgcgaaaga ttacccaagg
      181 gcgcgagcat gacatccgcc cctgtgtcgg cgccacctat tgcctcgacc gcatctatca
      241 gggcggggcg gcctactgca tccacaatcc ggcgaccggc cgcgagaccg agattccgca
      301 cgacctgccc aaggcggcca ggcggcgcaa gttcgtcgtc gtcggggccg ggccggccgg
      361 gctggaagcc gcgcgggtcg ctgccgaacg gggccatgaa gtggttgtgt tcgaggcggc
      421 aagccatgcc ggcggccaga tccggctgac cgcgcaatcc aggcgccggc gcgagatgat
      481 cggcatcatc gactggcgcg tagcgcaatg cacggccatg ggcgtcaaga tcaactacag
      541 cacatgggcc gacgccgcga cggtgcagcg cgaaaatccg gatgtcgtca tactggccac
      601 cggcggcatt ccgcatacgg aagtgttgag cgccggcaac gatctggtgg tgtcgtcgtg
      661 ggacattatt tccggcgacg taaagcccgg aacgaatgtg ctggttttcg acgacgccgg
      721 cgaccatagc ggcctgcaag cagccgaagt tatcgccgcc agcggcggca aggtcgaggt
      781 gatgacgccg gaccgctcgt tcgcgcccga agtgatggcc atgaacctcg tgccctatat
      841 gcgctcgttg cagaagctgg acgccacctt caccgtaacg ttccggctgg aggcggtgga
      901 gcgccaaggc aacggcctgg tcgccatcgt cggcagcgac tatggcgggg tgcgcaagga
      961 gcggcgtgtc gatcaggtcg tcgtcaacca cggcacggtg ccgctggacg agctttattt
     1021 cgagttgaag ccgcaatcgc gtaatctcgg cgtcctcgac catggcgatt tcatcgatgg
     1081 caggccgcag acaagcgcgg gcaatccgca aggcacgttc cagcttttcc ggatcggcga
     1141 cgccgtggcc tcacgcaaca cccacgcggc catctacgac gccctgcggc tggtgaagga
     1201 tctctagcgg cggaaaggcg gcatccgccc cgactatttg agctggagca gccgttcgag
     1261 atagctgcgc tcgatgtcgg gcgacagcgc attgccaagc cgcttgcgga tggcttccaa
     1321 tatctggcgc gcgcgctgga cgtcgatctc gtcgggcacc ttgaccgtgt ccccgaaatc
     1381 cggcccctgc gtggcgcgcg ggcggccgag cgggtcacgg tcggcatccc gccggccgcc
     1441 gccctgccgg cttccgcctt ggtcgccctg catggcctgc atctgcttca tcatgtcctt
     1501 ggcgcccttg cgcagctctt ccagcgcctt gccctggtgg ccgactgcgt tttcgccctc
     1561 accctcgccc agcgcctgct cggcgctgcc catcgatttg ccggcctggc cgaagccctt
     1621 gttcggctcc agtcccattc cttcgagacc cttcttcagc ttctcaaggt cgccttgaag
     1681 cttggactgg ccttcctgca actgcttgag ggcgtcggcg aattcttccg gggtcatagg
     1741 tttaccctcc ggagacttgc cttcctgctg ctcgccgttt cggccctgcc ggggttggtc
     1801 ctgccgggat tcgccttgcg ggggctggcc ctgatcgtca aactcgccga agggattttc
     1861 ctcgccctgt tcgcgccgct gctggccgcg ttgcgcctga tccgtgcgga acgtgtcgtt
     1921 catcatctcc tgctgccggc gcatgatctc gccaagcttg tccatctgcc ggcgcatctc
     1981 gctgtcctgc tggccctgct gctgctggcg gccggcctgg aggttgttca tcatgttgtc
     2041 gagctgcgac agcaattcct gcgccttgtc gcgattgccg gacttggcca ggttttcgat
     2101 ctggtccatc atcttttgca ggtcgctctg gcggatttcc ttgccgtcct gcggcatctg
     2161 cgcggatttc cggttctgct ggcgctgggc gaattccttg aggaactcgt tcatcgcctg
     2221 gcgcagttcc ttcatcgcct tgtcgatctc ctcgtcgccg gcgccgtttt tcaaggcatc
     2281 ctgcaatgcc tgctgcgcct ggcgcagccg cttttcggcg gccgaaagat cgccttcctc
     2341 gatgccgagc gcgatgtccc acagataggc gaccgtgtcg cgcagctggt cgtcgctgtc
     2401 ggccagtttt agccgcgtgc gggcgctcat gatggcgagg taatgcgcca tagtgtcgaa
     2461 cgtatcctgc gggcgcagcg taatggcatc catcagctca agcacatgag gcttggcgtt
     2521 ggcgtcgagc gccaaaaggc ggcgctgctc gatgacggcc agcgccagcg gattggtgaa
     2581 aggccgctgc ggcatcgtca gcgtccttgt ctcgctggtg gccatctgac cggcgtcatc
     2641 ggttgcgacg agcttgagcg tgacgggcgc accagcccag acgtgttcgg tcagatcctt
     2701 ggtcgttcga gccgcgttgg ccttgccgcc gcgtcggggc agcgccagcg gcatgtcggg
     2761 cggtccgtaa agcggatgcg catttgccgc cggcttttgc gccagttcga acacggcctt
     2821 ggccgaggcc gcgccatagt cgtcatcgat ctggtagcca agctcgatgg caccgttggc
     2881 ggcctgcttc gactcgccga caaagcgtat ctgcggtggc ttgtccggcg tcaccgtaaa
     2941 ggaccagcgg ccaagctcgc tgttgcccga tttcagcgtg agaatgccgt tggccttcag
     3001 cctggctgcg aactggcgga cattgtcgga ggcctggtcc gccggggtag cctgcgccgg
     3061 ggccgacttg ccggctgtcg gtgtcgcgga aggatcaagc tcgcggctgt tgccgccggc
     3121 gtcggcatag gcgagcgtct cgtcgccgga cccgccggta acgcgcaggg acaggtcgct
     3181 gccgaccggg atggaaatgg cggcgctgtc ggccgctcca gccgcgagga agaccggcgg
     3241 cttgccggtg tagaccggcg gcgtcaccca ggcatcgacg cgcggcggcg tggcgtcgcg
     3301 cgtcgcactg ggacgaaaac cgtcgcccac gctgccgccc agcgacccaa acgagaaggc
     3361 gaaggcgacg gcgagcgcca aagcggcgag cgcgcgcagg gcccacgggt cgcgctcggg
     3421 cacatgggtg cgcggcaggt ccgcgccaag atggccaagc gttgccgcca tgcgcttctg
     3481 atgttcgcgc cacagcgcct gtgaaaagcc ggtctcgacc ccgcttggcc ggtcggactg
     3541 tacctggacc gggctgtgcg gcagggcgtt tgccgcttcg atgcggcggt cgacttcgcc
     3601 tggcttcggc aggcggaaga agcgcagc
//
LOCUS       DASYCS010000267         2069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4272437,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000267 DASYCS010000000
VERSION     DASYCS010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4272437"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..1814)
                     /locus_tag="VGM46_08580"
     CDS             complement(183..1814)
                     /locus_tag="VGM46_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006330877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase family protein"
                     /protein_id="HEY4192690.1"
                     /translation="MASENRVEWWRGCAIYQIYPRSFQDTNGDGSGDLHGIVQRLGHA
                     ASLGVDAVWLSPFFKSPMADMGYDVCDYEAVDPLFGTIDDFDAVVAEAHRLGLKVIID
                     QVLAHSSDRHPWFIESRASRQNPKSDWYVWADAKPDGTAPNNWISVFGGPAWEWDSTR
                     RQYYLHNFLAAQPALNFHNMDVQNAVLETVQFWLKRGVDGFRLDTVNYYVQDRWLRSN
                     PPLPQSVAGTNGDTNPYNFQEHLFDKSRPENIDFLKRFRALIDEGEGHAVVGEVGDES
                     RSLQTLAAYTSGNDKLNMCYTFDMLGPEFSAAHVRGCVEAFEAAAPSGWVCWAFSNHD
                     VVRHVTRWTRPGGDPVAVAKFSIELLCALRGSICLYQGEELGLEEADIAFEDLRDPYG
                     IRFWPGFKGRDGCRTPMVWEAGAANAGFSSAKPWLPVPDGQRAKAVDAQSGDGNSVLA
                     HYKAVLALRRAHPALVTGSIRFLDAEGDLMAFIREGGGEKLLCVFNFASQPARWQLPA
                     QLGPVEAVDFAGYGASVQNGALELPELRCFIGQLY"
     gene            complement(1836..>2069)
                     /locus_tag="VGM46_08585"
     CDS             complement(1836..>2069)
                     /locus_tag="VGM46_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme GlgX"
                     /protein_id="HEY4192691.1"
                     /translation="QADWNDPGRRRLVMLLDGGEGQRLAVLVNGDRRACQFTLPKRAG
                     HAWMPALAPSVEPEAASLLVAGRSVIFMIEREA"
BASE COUNT          388 a          681 c          649 g          351 t
ORIGIN      
        1 agcggccttt taggcccgct caagccgctg ctgctgccga cgctgaatgc ggcgaccgcg
       61 cctctggacc agttgctggg aaccgtgctc gacgcccttg gcataaaggt cggcaaggcc
      121 gatgtgcgcg tgacgggggc cacctgcgac cgctccgttc tggtgcaata aacccgccct
      181 tgtcagtata gctggccgat gaagcagcgc agttccggca gttccagcgc gccattttga
      241 acggaggcgc cgtagcccgc aaaatccacc gcctcgaccg gaccgagttg cgccggcagt
      301 tgccaacgcg ccggctggct ggcgaaattg aagacgcata gcagtttttc gccgccgccc
      361 tcgcggatga aagccatgag atcgccctcg gcgtcgagaa agcggatgga gccggtgacg
      421 agcgccggat gggcgcgccg cagcgccagc acagccttgt aatgggcaag caccgaattg
      481 ccgtcgccgg actgggcgtc cacggccttc gcgcgctggc cgtccggtac cggcagccag
      541 ggcttggcgc tcgaaaagcc ggcattggcc gcacccgcct cccacaccat cggcgtgcgg
      601 cagccatccc tgcccttgaa gcccggccag aagcggatgc catagggatc gcgcaaatcc
      661 tcgaaggcga tatcggcttc ctccagcccg agttcctcgc cctggtagag gcagatcgac
      721 cccctcagcg cgcatagcag ttcgatcgag aacttcgcca cggccaccgg gtcgccgccc
      781 ggccgcgtcc agcgcgttac gtggcgcacc acgtcatggt tggaaaacgc ccagcagacc
      841 cagccggacg gcgcggcggc ttcgaacgcc tcgacacagc cgcgaacatg ggccgccgaa
      901 aactccggcc ccagcatgtc gaaggtgtag cacatgttga gcttgtcatt gcctgacgtg
      961 taggcggcca gcgtctggag cgagcggctt tcatcgccga cctcgccgac cacggcatgg
     1021 ccctcgcctt cgtcgatcaa ggcgcggaag cgtttcagga aatcgatgtt ctcaggccgc
     1081 gacttgtcga acagatgctc ctggaaatta taggggttgg tgtcgccatt ggtgccggcg
     1141 acgctttgcg gcagaggtgg attgctgcgc agccagcgat cctgaacgta ataattgacg
     1201 gtatccagcc gaaaaccgtc gacaccgcgc ttcagccaga attgcaccgt ttccagcaca
     1261 gcgttctgca cgtccatatt gtggaagttg agtgccggct gcgccgccag gaaattgtga
     1321 aggtagtatt ggcggcgtgt cgaatcccat tcccaagccg ggccaccgaa caccgaaatc
     1381 cagttgttgg gcgcggtgcc gtccggcttg gcatcggccc agacatacca gtccgatttc
     1441 gggttctggc ggctggcgcg gctttcgatg aaccatggat ggcggtcgga ggaatgcgcc
     1501 agcacctggt cgatgatgac tttcaggccg agcctgtgcg cctcggcaac gaccgcgtcg
     1561 aaatcgtcga tggtgccgaa cagcggatcg acggcttcgt agtcgcagac atcgtagccc
     1621 atatcggcca tcggcgattt gaagaagggc gacagccaca ccgcatccac gccgagcgac
     1681 gcggcatggc caagccgctg gacgatgccg tgcagatcgc cgctgccatc gccattggtg
     1741 tcctggaagg agcgcggata aatctggtag atggcgcagc cgcgccacca ctcgaccctg
     1801 ttttcacttg ccatcaacac tgcccctttg cgccctcaag cttcgcgctc gatcatgaaa
     1861 atcacgctac gcccggcgac aagcagcgat gcggcctcag gctcgaccga gggggcgagc
     1921 gcgggcatcc atgcatggcc cgcgcgcttc ggcagcgtga actggcaggc gcggcggtcg
     1981 ccgttgacaa gaacggcaag ccgctgcccc tcgcccccgt caagcagcat gaccaggcga
     2041 cgtcgccccg gatcgttcca atcggcctg
//
LOCUS       DASYCS010000268         5844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2531998,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000268 DASYCS010000000
VERSION     DASYCS010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 5844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5844
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2531998"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VGM46_08590"
     CDS             <1..237
                     /locus_tag="VGM46_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEY4192692.1"
                     /translation="YALWLYRRIIYGALTKDSLKAMLDLSGREKAILYPLVILVIFFG
                     VYPAPVLDATAQSVKSLVTNVTASIDAAHAAAAK"
     gene            249..1682
                     /gene="nuoN"
                     /locus_tag="VGM46_08595"
     CDS             249..1682
                     /gene="nuoN"
                     /locus_tag="VGM46_08595"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEY4192693.1"
                     /translation="MTPDLYSSLSLATPELIVAVGALALLMLGAYWRGNASTIVTSLA
                     VLVMIAAGAWIVLFTGDGHAFNNAFISDPFARFMQVLTLVGSAVTLVMSLGFAKAEKF
                     DTFEYPVLVMLSTLGMLLMASANNMISLYISFELQSLGLYVVAAINRENLRSTEAGLK
                     YFVLGALASGMMLYGISLVYGYTGSVEFGVIRDAVGADRQVGVLFGLVFILAGLVFKI
                     SAAPFHMWTPDVYEGAPTPVTAFFAVAPKVATMALILRITMFAFEPVAADWQQIIVFV
                     SIASMALGSFAAIGQQNFKRLMAYSSIGHMGFALVGLAANSQAGVRGVIIYMLIYVAM
                     TLGTFAFIIAMRRKEGQVETIGDLAGLSTTNPVMATILTILLFSLAGIPPMAGFWAKW
                     YVFLAAINSGLYALAIIGVLTSVVAAFYYLRIIKIMWFDEPVGGFLPMSGTLRLVLGL
                     SGAFVLLYVFIGGPIGTLAQAAAKSFF"
     gene            1691..2512
                     /locus_tag="VGM46_08600"
     CDS             1691..2512
                     /locus_tag="VGM46_08600"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006335660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEY4192694.1"
                     /translation="MAFRLAPTSEAEGFRLDALETVGSTNAYALDLARAGDPGKVWVV
                     SKKQENGRGRRGRAWQSPEGNLAATLLINIGGDLRLAATLGFVAGLALGDALDAVAPG
                     SRVSIALDGASAGQNRFELKWPNDVLASGAKLAGILLESLVLDDGHFAIAVGIGVNVV
                     AHPQDLPYPATSLHALGCKCDAETLFLALSDAWSANARLWSEGNGIGAIRERWLARAA
                     GLGGQVAVRVGGDVVRGTFETIDEDCRFVIRDDSGAAITIAAGDVHFGAVASAGP"
     gene            2575..4245
                     /locus_tag="VGM46_08605"
     CDS             2575..4245
                     /locus_tag="VGM46_08605"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEY4192695.1"
                     /translation="MAKVNAPELVFVPLGGVGEIGMNFALYGYGPANAREWIIIDVGV
                     TFPDDTLPGVDLVLPDTRFIEGNLDRLHGIVITHAHEDHYGALLDIWPKLKVPVWMTP
                     FSAGLLDAKRQGEHGAPKIPVTVFRGGEKFTVGPFEIEAIPVAHSIPEPMSLAITTPA
                     GVVIHTGDWKIDPAPTVGPKTDEARFRAYGDKGVLALICDSTNAMREGDSPSEQAVGE
                     GIKGVIESAVGRVAVTTFSSNVGRIVSIAKAARDAGRQCLVLGRSLKRVIDVAGELGY
                     MDGLPEFISEEDYGFIPRENLVIICTGSQGEPLAALAKLSRGEMKAVALTAGDTVVFS
                     SRTIPGNEKAILAIKNGLIDLGMKIIEDGDALVHVSGHPRRSELKQMYSWVRPQIGVP
                     VHGEAAHLAAQGSLMAISGIGQVAQVRDGDMLRLAPGAATIIDKVPFGRIYKDGRLMG
                     TDQAMGIRDRRKLSFAGHVVVNVVLDEKYELAGDPDLVAIGVAETDARGETLEDLMLD
                     AAIGAVDSIPRQRRKDLNLVQEAVRRAVRGAANEAWGKKPLATVFVTR"
     gene            4268..4672
                     /gene="mce"
                     /locus_tag="VGM46_08610"
     CDS             4268..4672
                     /gene="mce"
                     /locus_tag="VGM46_08610"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007626175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HEY4192696.1"
                     /translation="MLGRLNHVAIAVPELEVATALYRDTLGARVTQPQALPEHGVTVV
                     FVDVGNTKIELLEPLGEGSPIAAFLQKNPAGGMHHVCYEVDDILAARDRLKASGARVL
                     GDGNPKTGAHGKPVLFLHPKDLFGTLVELEQS"
     gene            4669..4932
                     /locus_tag="VGM46_08615"
     CDS             4669..4932
                     /locus_tag="VGM46_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1467 family protein"
                     /protein_id="HEY4192697.1"
                     /translation="MNWISFTALFFITWWTTLFAVLPFSVRTQEDDQNVTLGTVASAP
                     RGPHMRRAVVRTTIVTAVIMGIAYFVTGYLGLGIDDIPHIVPD"
     gene            complement(5112..5333)
                     /locus_tag="VGM46_08620"
     CDS             complement(5112..5333)
                     /locus_tag="VGM46_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192698.1"
                     /translation="MKENGARNRCAAAACMGFIAISWRDGNPRRARRKVTTLRLIFVC
                     AALPIYCATQQRNAYFKAKFSVTAWLALG"
     gene            5344..>5844
                     /locus_tag="VGM46_08625"
     CDS             5344..>5844
                     /locus_tag="VGM46_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019858921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HEY4192699.1"
                     /translation="MRLSRYFLPILKENPREAEIVSHRLMLRAGMIRQQAAGSFSWLP
                     LGKRVLDKICAIVREEQDRAGALEILMPTIQSADLWRESGRYDDYGKEMLRIKDRHDR
                     DMLFGPTNEEMVTEIFRSYVKSYKDLPLNLYHIQWKFRDEVRPRFGVMRSREFLMKDA
                     YSFDLDF"
BASE COUNT         1031 a         1753 c         1857 g         1203 t
ORIGIN      
        1 tatgcgcttt ggctctaccg gcgcatcatc tacggggcgc tgaccaagga tagcctgaag
       61 gccatgctgg acctgtcggg gcgtgaaaag gcgatccttt atccgctcgt gatcctcgtc
      121 atcttctttg gcgtatatcc agcgccggtc ctcgatgcga cggcccagtc ggtcaagagc
      181 ctcgttacca acgttactgc atccatcgac gcggcgcatg ccgcggcggc gaaatgaccg
      241 ggggaaccat gacgccggac ctttactcca gtctttcgct cgcgacgccg gaactgatcg
      301 tcgccgtcgg cgctttggcg cttctgatgc tcggcgctta ttggcgcggc aatgccagca
      361 ccatcgtcac ctcgctggcg gtgctggtta tgatcgcagc cggcgcgtgg atcgtgctgt
      421 tcaccggcga cggacatgcc ttcaacaatg ctttcatcag cgatcccttt gcccgcttca
      481 tgcaggtttt gacgctagtc ggctcggctg tcacgctggt catgtcgctg ggcttcgcca
      541 aggccgagaa attcgacacg ttcgaatatc cggtgctggt gatgctgtcg acgctgggca
      601 tgttgctgat ggcgtcggcc aacaacatga tttcgctcta cattagcttc gagcttcagt
      661 cgctggggct ttacgtggtg gcggcgatca accgcgagaa cctgcggtcg acggaagcgg
      721 gcctgaaata tttcgtgctg ggcgcactgg cgagcggcat gatgctttac ggtatctcgc
      781 tggtctatgg ttataccgga tcggtcgagt tcggtgttat ccgcgatgcc gtcggcgctg
      841 accggcaggt cggcgtgctg ttcggcctgg tgttcatcct cgctggcctg gtgttcaaga
      901 tttcggcggc tccgttccat atgtggacgc cggacgtcta tgagggcgca ccgacgccgg
      961 tgacggcttt cttcgccgtg gcgccgaagg tggcgaccat ggcgctgatc ctgcgcatca
     1021 ccatgttcgc cttcgagccg gtcgcggcgg actggcagca gatcatcgtc ttcgtgtcga
     1081 tcgcctcgat ggcgctcggt tccttcgcgg caatcgggca gcagaatttc aagcgcctga
     1141 tggcttattc gtcaatcggc catatgggct tcgcgctggt cggccttgcc gccaacagcc
     1201 aggctggcgt gcgcggcgtc atcatctaca tgctgatcta tgtcgccatg acgctcggca
     1261 cttttgcgtt catcatcgcc atgcggcgca aggaagggca ggtcgagacg atcggcgacc
     1321 ttgccggcct gtcgacaacc aatccggtga tggcgaccat tctcaccatc ctgttgtttt
     1381 cgcttgccgg catcccgcca atggccggct tctgggcgaa atggtatgtg ttcctcgcag
     1441 ccatcaattc cggcctctac gctctggcta tcatcggcgt gctgacctcg gtggtggcgg
     1501 cgttctacta tctgcgcatc atcaagatca tgtggttcga tgagccggtc ggtggcttcc
     1561 tgccgatgtc gggcacgcta cggctggtgc ttggcctgtc gggcgcgttc gtcctgcttt
     1621 acgtgttcat cggcggaccg attggcacct tggcgcaggc cgccgccaag agtttcttct
     1681 gacgggcgcc atggcatttc gcctggctcc gacatccgag gcggaaggct tccgtctcga
     1741 tgcccttgaa acggtcggtt cgaccaatgc ctatgctctc gacctggccc gagccggcga
     1801 tcccggaaag gtttgggtcg tttccaaaaa gcaggaaaac ggacggggcc ggcgcggccg
     1861 cgcctggcaa tcgccggaag gcaatctggc ggcgacgctg ctgatcaata tcggcggcga
     1921 ccttaggctt gcggcgacgc tgggctttgt cgccgggctg gccttgggcg acgcgctcga
     1981 cgccgtggct cccggcagcc gggtttcgat agcgctcgac ggtgcaagcg ccggacaaaa
     2041 tcgcttcgaa ttgaaatggc cgaatgacgt gctggcctcg ggcgcaaagc ttgccggcat
     2101 cctgcttgaa tccttggtgc tcgatgacgg gcactttgcc attgcggtcg gcatcggcgt
     2161 caatgtagtg gcccatccgc aggatttgcc ctatccggcg acttcgctcc atgcgctcgg
     2221 ctgcaaatgc gatgcggaga cgttgtttct ggcactctcg gatgcctgga gcgccaatgc
     2281 gcggctttgg agcgaaggaa acggaattgg tgccattcgc gagcgctggc tggcccgcgc
     2341 ggcggggctt ggcggacagg tcgccgtcag ggttggcggc gatgtggtgc gcggaacctt
     2401 cgagacgatc gacgaggatt gccgtttcgt catccgcgac gatagcggcg cggcgataac
     2461 gattgccgcc ggcgatgtac atttcggcgc ggtcgcttcg gccggtcctt gattgacggc
     2521 atttaacctg tcaggacgca ggttccggcc ccgccggcaa gaaaaggaca gaccatggcg
     2581 aaagtgaacg cacccgaact cgtattcgtg ccgcttggcg gcgtcggcga gatcggcatg
     2641 aatttcgccc tctatggcta cggcccggcc aatgcgcgcg agtggatcat catcgacgtg
     2701 ggcgtgacct ttcccgacga caccttgccc ggcgtcgatc tggtgttgcc cgacacgcgc
     2761 ttcatagagg ggaacctcga ccggctgcac ggcatcgtca tcacccatgc gcatgaagat
     2821 cactatggcg cgttgctcga catctggccg aagctgaagg tgcctgtctg gatgacgccg
     2881 ttttcggcgg ggctgctcga cgccaagcgg cagggcgaac acggcgcgcc gaaaatcccg
     2941 gtgaccgttt tccgcggcgg tgagaaattc acggtaggcc cgttcgagat cgaagctatt
     3001 ccggtcgcgc actccattcc agaacccatg tcgctggcga tcacgacgcc cgccggcgtc
     3061 gtcatccaca ccggcgactg gaagatcgat ccggcgccga ccgtcgggcc gaagaccgac
     3121 gaggcgcgct tccgcgccta tggcgacaag ggcgtgcttg cgctcatctg cgattccacc
     3181 aacgccatgc gggaagggga ttcaccctcc gagcaggcgg tgggcgaagg catcaaaggc
     3241 gtgatcgaga gcgctgtcgg ccgcgtcgcc gtcactactt tttcgtccaa tgtcgggcgc
     3301 atcgtttcca tcgccaaggc ggcgcgcgat gccggccggc aatgcctggt ccttggccgc
     3361 tcgctgaagc gcgtcatcga tgtggccggc gaacttggtt acatggatgg cctgcccgaa
     3421 tttatcagcg aggaagatta cggcttcatt ccgcgcgaaa acttggtcat catctgcacc
     3481 ggcagccagg gcgaaccgct ggcggcgctg gcaaagcttt cgcgtggcga gatgaaggcc
     3541 gtggcgctga ccgccggcga tacagtggtg ttctcgtcgc gcaccattcc cggcaacgag
     3601 aaggcgatcc tggcgatcaa gaacggattg atcgatctcg gcatgaagat catcgaggat
     3661 ggcgatgcgc tggttcatgt ctccggccat ccccgccgca gcgaactgaa gcagatgtat
     3721 tcgtgggtgc gtccgcaaat cggcgtgccg gtgcatggcg aggcggcgca tctggcggcg
     3781 cagggttcgc tgatggcgat ttcaggcatc gggcaggtcg cgcaggtgcg cgacggcgac
     3841 atgctgcggc tggcgccggg tgcagcgacc atcatcgata aggtgccctt cgggcgcatc
     3901 tacaaggatg gccgcctgat gggcaccgac caggcgatgg gcatccggga ccggcgcaag
     3961 ctttcctttg ccggccacgt cgtggtcaat gtcgtgctgg acgagaaata cgagcttgcc
     4021 ggcgaccccg acttggtggc catcggcgtg gccgagacgg atgcgcgcgg cgagacgctg
     4081 gaggatctga tgctcgatgc ggcaatcggc gctgtggact ccataccgcg ccagcgccga
     4141 aaagacctga acctcgtgca ggaagcggtg cgccgcgccg ttcgcggcgc cgccaacgaa
     4201 gcctggggca agaagccgct ggcaacggtg ttcgtgacgc gctgacgggg gcgggagaag
     4261 cagggaaatg cttggacgac tgaaccatgt cgcgattgcc gtgccggagc tggaggtggc
     4321 gacggccttg tatcgcgata cgctgggcgc cagggtgacc cagccgcagg cactgccgga
     4381 gcatggcgtg acggttgtgt tcgtcgatgt cggcaacact aagatagagt tgctcgaacc
     4441 gcttggcgaa ggctcgccga tcgccgcgtt tcttcagaaa aacccggcgg gcggcatgca
     4501 tcatgtctgc tacgaggttg acgacattct ggcggctcgc gatcggctca aggccagcgg
     4561 cgcgcgggtg ctgggcgatg gaaatcccaa gaccggcgcg catggcaagc cggtcctgtt
     4621 cctccacccc aaggatctct ttggaaccct ggtcgaactg gagcaatcat gaactggatt
     4681 tcgttcactg ccctgttttt catcacctgg tggacaacgc tgtttgccgt tttgccgttc
     4741 agtgtcagaa cgcaggaaga cgaccagaac gtcacgctcg gcaccgtcgc cagcgcgccg
     4801 cgtgggccgc atatgcggcg ggcagtggtg cgcaccacca tcgtcaccgc cgtcatcatg
     4861 ggcatcgcct atttcgtcac gggctacctc ggcttgggta tcgatgacat tccgcatatc
     4921 gttccggact gaataaggct catcattcag gcacaaaaaa aagcaaggcg cgaagccttg
     4981 ccaaataaac gcgtccgacc ttaatccagt tattggcggc tgcgagttac cgcgcccaga
     5041 tcgcatcctc ccaagacttt gaccgcgcag gagggtagat ttgtctctac cccctcggtt
     5101 atgcgggtac actaacctaa tgccaaccat gctgtcacgg aaaattttgc tttgaaataa
     5161 gcattccgtt gttgcgttgc acaataaatc ggcagcgccg cgcacacgaa aatcagccgt
     5221 aatgtcgtga ccttgcgccg cgcgcggcgc gggtttccat cccgccatga aatggctatg
     5281 aagcccatgc aagcagcagc ggcgcagcga ttccttgcgc cattctcttt catggaccag
     5341 atcatgcggt tgtcgcgtta tttcctgcct attctcaaag aaaatccgcg cgaagccgag
     5401 atcgtttcgc accggctgat gctgcgcgcc ggtatgatcc ggcagcaggc ggcgggcagt
     5461 ttctcttggc tgccgctggg caagcgcgtg ctggataaaa tctgcgccat cgtgcgtgag
     5521 gaacaggaca gggccggcgc cctcgaaatc ctcatgccga ccatccagtc ggccgatctg
     5581 tggcgcgaaa gcggccgcta cgacgactat ggcaaggaga tgctgcgcat caaggaccgg
     5641 catgaccgcg acatgctttt cggcccgacc aacgaggaga tggtaacgga aatcttccgt
     5701 tcctacgtga agtcctacaa ggatttgccg ctcaacctct accacatcca gtggaagttc
     5761 cgcgacgagg tgaggccgcg cttcggcgtc atgcgttcgc gcgaattcct gatgaaggac
     5821 gcctattcgt tcgacctcga tttc
//
LOCUS       DASYCS010000269         1927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2822961,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000269 DASYCS010000000
VERSION     DASYCS010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2822961"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VGM46_08630"
     CDS             <1..306
                     /locus_tag="VGM46_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEY4192700.1"
                     /translation="FEKLRLSSGDGIILLMLVTSGLAHLPPIKVVTILAGVIGFSLPW
                     FIVSAVVARGARFLFLAWLLRRYGEPIRDFIEKRLGMIAGIAAGVLILLYIIVKYAI"
     gene            339..854
                     /locus_tag="VGM46_08635"
     CDS             339..854
                     /locus_tag="VGM46_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006328500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEY4192701.1"
                     /translation="MISTIDAAHSRRKLTALFLAVAMAAVVGGALAFQYIGGYIPCKL
                     CYEQRIPYYVGVPLMLLAALAAALRLPSWTSRILLAIGSLLMLYGLYLAVYHSGVEWS
                     WWPGPTDCTNVASPVDTGGNGVLDAIDKFVPPSCDKAALRILGLSMAGWNAIASLILS
                     IVALRGAAARG"
     gene            complement(851..>1927)
                     /locus_tag="VGM46_08640"
     CDS             complement(851..>1927)
                     /locus_tag="VGM46_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY4192702.1"
                     /translation="ISSDLIAEGILNESRDAPPSLKRGRPQVGLALNARATSVLSVVL
                     SLNFLSIAVIDYAGGVIAQEERRLDTQAMSREQLVAECVAIIRHRLDDPSFAGGKVAR
                     LVLAVQGTTDAAARIVLWSPITPQTDIAFAAILEEEFGIPVAVENDCNMMAVALRWRD
                     PARYRDDFIAILLSHGIGMGIVLKGELFTGTHSSGGEFGHMVHRPNGALCRCGRRGCV
                     EAYAGNYAIWRNARQGSEDEEPLADICDADIQELAAIARDHDGREREAFRKAGEALGF
                     GLGSLFALIDPAPVAMVGIGATAFDLIEPALREAIGRTAGGHHSKAISFDTEPNELSL
                     IHEGCAMRALVFVDQEIFAQGGQT"
BASE COUNT          317 a          657 c          595 g          358 t
ORIGIN      
        1 ttcgagaagc tgcgcctgtc ctccggcgac ggcatcatcc tgctcatgct ggtcacttcc
       61 ggccttgcgc atttgccgcc gatcaaggtg gtgaccatcc tggccggcgt catcggcttt
      121 tccctgccct ggttcatcgt ctcggcggtc gtggcgcgcg gcgcgcgctt cctgtttctg
      181 gcgtggctgc ttcgccgcta tggcgaaccg atccgcgact tcatcgaaaa acgcctgggc
      241 atgatcgccg gcatcgccgc cggcgtcctt attctgctct atatcatcgt caaatacgcc
      301 atctgagcat tgccatcggc acgacccgga gcgcaaccat gatctcgacc atcgacgcgg
      361 cccacagccg ccgcaaactg acggccctgt ttctggccgt cgccatggcc gccgtggtgg
      421 gcggcgcctt ggccttccag tacataggcg gctatatccc gtgcaagctg tgctacgaac
      481 agcgcatccc ctactatgtc ggcgttccgc tgatgttgct ggcggccctg gcggccgcgc
      541 tgcgcctgcc ttcatggact tcgcgtatac tgctggccat cggcagcctg ctgatgctct
      601 acgggctata tctggccgtt tatcattcag gcgtcgaatg gtcatggtgg cccgggccga
      661 ccgactgcac gaatgtagcc agcccggtcg atacgggcgg caacggcgtg ctcgatgcga
      721 tcgacaagtt cgtgccgcca tcctgcgaca aggcggcgct gcgcatcctc ggcctctcca
      781 tggccggctg gaacgccatc gccagcctga tcctgtccat cgtggcgcta cggggcgccg
      841 ccgcccgcgg ctaggtctgg ccgccctgcg cgaagatttc ctgatcgaca aaaaccaggg
      901 cgcgcatggc gcagccttcg tggatcaagg acaattcgtt cggctcggta tcgaacgaga
      961 tcgccttcga gtgatggccg cccgcggtcc tgccgatagc ttcgcgcagc gccggctcga
     1021 tgagatcgaa cgcggtcgcg ccgatgccga ccatggccac cggcgcggga tcgataagcg
     1081 caaacaggct gccaaggcca aagcccagcg cttcgccggc cttgcgaaac gcctcgcgct
     1141 cgcgaccgtc atggtcgcgc gcgatggccg ccagttcctg gatatcggca tcgcagatgt
     1201 cggcaagcgg ctcttcatct tcgctgccct gccgcgcgtt tcgccagatg gcgtagttgc
     1261 cggcataggc ttcgacgcag ccgcgccgtc cgcagcggca gagcgccccg ttcggccggt
     1321 gaaccatatg gccgaactcg ccaccggatg aatgcgtgcc ggtgaacaac tcgcccttca
     1381 gcacgatgcc catgcctatg ccgtgcgaaa gcaggatggc gatgaagtcg tcgcggtagc
     1441 gggccgggtc gcgccagcgc agcgccaccg ccatcatgtt gcagtcattc tcgacggcga
     1501 ccggaatgcc gaattcctcc tccagtatgg cggcgaaggc aatgtcggtc tgcggcgtga
     1561 tcggcgacca caatacgatg cgcgcagccg catccgtggt gccctggacg gcaagcacga
     1621 gacgcgccac cttgcccccg gcaaaactcg gatcgtcaag acggtggcgg atgatcgcca
     1681 cgcattcggc gacaagctgc tcgcgcgaca tggcttgcgt gtcgagccgg cgctcctcct
     1741 gcgcgataac cccgcctgca tagtcgatga cggcgatgga aaggaaattc agcgacaaca
     1801 cgacgctcag caccgatgtc gcccgcgcgt tcagcgccag ccccacctgc gggcgtccgc
     1861 gcttcagcga gggcggggca tccctgcttt cgttgaggat accctcggcg atcaggtcgg
     1921 atgaaat
//
LOCUS       DASYCS010000270         2758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2678263,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000270 DASYCS010000000
VERSION     DASYCS010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2678263"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VGM46_08645"
     CDS             complement(<1..180)
                     /locus_tag="VGM46_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEY4192703.1"
                     /translation="MADVALPGSIEPAAHKGPKLDGAVNPLQGIIYAGVIAAALLFVA
                     YSIYSDVDATGTRVTS"
     gene            complement(443..1906)
                     /locus_tag="VGM46_08650"
     CDS             complement(443..1906)
                     /locus_tag="VGM46_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687447.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HEY4192704.1"
                     /translation="MHLITLDSETDFEGWRKAARSLVLHEVKPTDVTWTVQGDASELF
                     APAAETLPLETPQDTFSVAAKFVELAQSAILHRNPERFAILYRLLWRLRGHPHLLEVA
                     TDPDVALVTAMAKAVRRDEHKMHAFVRFREVGREQKSHYVAWFEPEHHIVELAAPFFA
                     RRFADMPWSILTPDACAHWDGHTVSITPGVSKADAPTEDRLEETWRRYYASIFNPARL
                     KVKAMQTEMPKKYWRNLPEASLIKPLIAGAERATGAMIANAATEPHKSQKRLEPSMTS
                     RREALRSDIETGAIATLREEAAGCRACPLWKDATQTVFGEGPAHARIMLVGEQPGDKE
                     DLAGKPFVGPAGQMLDRALQEAGIDRSKVYVTNAVKHFKFVPRGKIRLHQKPGTPEIR
                     ACRPWYERELAAIKPALVVAMGATAAQSVFGRITPINKNRGQLIELDHGIQALVTVHP
                     SYLLRLPDADAKAREYERFVNDLRIAAALLKKNAHAA"
     gene            complement(2075..>2758)
                     /locus_tag="VGM46_08655"
     CDS             complement(2075..>2758)
                     /locus_tag="VGM46_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008136650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEY4192705.1"
                     /translation="VRAIRRTMGRLRLKLDEAQEDQKTATKAKPPRFAPAGQSTQMIV
                     GADSANDQTILDTSANLYGSYKLKRVYYSAFSPIPDASRALPLSAPPLVREHRLYQAD
                     WLMRFYGFDADEIVDPTQGMLSLEIDPKLAWALRHRHRFPLDIASASRADLLRVPGFG
                     TKAVDRIIATRRVTSIRVSDLARLHIPAKKALPFIVLSDHRPAMHLLDEAKLAERFKP
                     KAKQLGFGF"
BASE COUNT          509 a          785 c          898 g          566 t
ORIGIN      
        1 ggaggtaacg cgggtgccgg tggcatcgac gtcggaatag atgctgtagg ccacgaacag
       61 caacgcggct gcgatcacgc cggcataaat gatgccttga agtggattga cggccccgtc
      121 gagcttgggt cctttgtgag cggccggttc tattgaacca ggtaatgcaa catcagccat
      181 agttaatact ccccagaaat cgatgggcgg acattgcttg aggcatgtga cagccacatg
      241 aaacgggcgc gaaagtcgtg tgttttcagg gcattgaatc gagtggagac aggcccgatt
      301 gaagcgggcc gtttccgcag cttctcgttg gaatcgctaa gacattgcgc gggggagagg
      361 tcgagaggtt agatcgaacc ggtatttagt tacgcatcgc catgaagcac gttcattgcg
      421 ccgcaataaa ttaagctaca tctcaagccg cgtgcgcatt cttctttagc agcgcggcgg
      481 caatcctgag gtcattgacg aaacgctcat actcgcgtgc cttggcatcg gcgtcgggca
      541 atcgcaacag ataggatgga tgcaccgtca ccaacgcctg gatgccatga tcgagttcga
      601 tcaactgccc gcggttcttg ttgatcggcg tgatccttcc gaaaacgctt tgcgccgcgg
      661 tagcgcccat ggccaccacg agcgcgggtt tgatcgcggc cagttcgcgt tcataccatg
      721 gacggcaggc ccggatctcc ggcgtgcccg gcttctggtg caggcggatc ttgccgcgtg
      781 gcacaaactt gaaatgcttc acggcgttgg tgacgtaaac cttcgatcgg tcgatgccgg
      841 cttcctgtag cgcgcggtcg agcatctgcc cggcgggacc gacgaacggt ttgccggcga
      901 ggtcttcctt gtcgccgggc tgctcgccaa ccagcatgat gcgggcgtgc gccgggcctt
      961 cgccgaacac ggtctgcgtg gcatccttcc agagcgggca ggcgcggcaa ccggcggctt
     1021 cttcgcgcaa ggttgcgatt gcgccggttt cgatatcgct ccttagagcc tcgcgtcggc
     1081 ttgtcatcga aggttccaac cgtttctgcg atttatgcgg ttcagtggca gcattggcga
     1141 tcatcgcgcc ggtggcgcgt tcggcgccgg cgatcagggg tttaatcaac gaagcctccg
     1201 gcaggttccg ccagtatttc ttcggcatct cggtctgcat ggccttcacc ttgagccggg
     1261 ccggattgaa gatgctggcg tagtaacgcc gccaggtttc ctccaggcgg tcttcggtcg
     1321 gcgcatcggc cttgctgacg ccaggcgtga tcgagacggt atggccgtcc caatgcgcgc
     1381 aggcatccgg cgtcaggatc gaccacggca tatcagcgaa acggcgggcg aaaaacggag
     1441 ctgccagttc gacgatatga tgctcgggtt cgaaccaggc gacgtagtgc gatttctgtt
     1501 cgcgcccgac ttcgcggaag cggacgaagg catgcatctt gtgttcatcg cggcgcacgg
     1561 ccttggccat cgcggtaacc agcgcgacat ccggatcggt cgcgacctcg agcaaatggg
     1621 gatgcccgcg cagccgccac agcagccgat acaggatcgc aaaccgctcg ggattgcgat
     1681 gcaggatcgc ggattgcgcg agttcgacga atttggcggc gacggagaag gtgtcttgtg
     1741 gggtttcaag cggcagcgtt tcggccgcgg gcgcgaacaa ttcggaggcg tcgccttgga
     1801 cggtccaggt cacatcggtt ggtttgactt catgcaggac cagcgatcgc gccgctttgc
     1861 gccagccttc gaagtcggtt tcgctatcga gggtgatgag atgcatggtt ttactctcct
     1921 tgcgacatac tcatcgtcat cctgaggagc cgcgcttgcg cggcgtctcg aaggatgtgc
     1981 cacggattcg tccttcgagg ctcgcaagag ttcgcacctt cagcgataac ggcgaagccg
     2041 ttacgcgggg atgacggtgg ggtgcgttgc acggttaaaa cccaaatccc aattgctttg
     2101 cctttggctt gaaccgctcc gccagcttcg cctcatcaag cagatgcatc gcgggccggt
     2161 gatcactgag aacaatgaac ggcagcgcct ttttcgccgg aatatgcagc cgcgccagat
     2221 cggacacgcg aatggacgtt acgcggcggg tggcgatgat gcggtcgact gccttggtgc
     2281 cgaagccggg cacgcgcagc agatccgcgc ggctggcgct ggcgatatcg agcgggaagc
     2341 ggtggcggtg ccgcagcgcc caggccagct tcggatcgat ctcgagcgac agcatgcctt
     2401 gcgtcgggtc gacgatttca tcggcgtcaa agccgtaaaa ccgcatcagc cagtcggcct
     2461 gataaagcct gtgctcgcga accaagggcg gcgcgctcaa cggcaacgcg cggctggcat
     2521 ccgggatcgg gctgaacgcg gagtaataca cgcgcttgag cttgtaggag ccgtagagat
     2581 tggctgacgt atcgaggatg gtctgatcgt tggcagaatc cgcgccgacg atcatctgcg
     2641 tgctctggcc ggccggggcg aagcgtggcg gttttgcttt ggtcgcggtc ttttgatcct
     2701 cctgcgcctc atcgagtttg agacgcagcc ggcccatggt gcggcggatt gcacgcac
//
LOCUS       DASYCS010000271         5184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4126759,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000271 DASYCS010000000
VERSION     DASYCS010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 5184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5184
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4126759"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3552)
                     /gene="gltB"
                     /locus_tag="VGM46_08660"
     CDS             complement(<1..3552)
                     /gene="gltB"
                     /locus_tag="VGM46_08660"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HEY4192706.1"
                     /translation="MTELTLAATNGQAAQTNAASVKRAALRSTTGMAPQGLYHPRNEH
                     DGCGVGFIANMKNEKSHGIVVDGLAMLENLTHRGAVGADPLMGDGAGVLVQIPDRFFR
                     EELARQDIELPPVGQYGVGHWFMPQDEALRTHIDEIIRESAQSEGLPLIGFRDVPVDN
                     SSLSKSPEIAAAEPFHRQVFIGRTPDIDDDEEYEARLYLLRKVISGRIYAENDNKDIG
                     AYCVSLSARTIVYKGMFLAYQVGAYYKDLADPRFETALILVHQRFSTNTFPSWKLAHP
                     YRMVAHNGEINTVRGNNNWMAARQASVDSELFGNNISKLWPISYEGQSDTACFDNALE
                     FLFQGGYSLAHSMMMLIPEAWAGNKLMGADRKAFYEYHAALMEPWDGPAAVAFTDGRQ
                     IGATLDRNGLRPARYIVTDDDRVIMASEAGVLDVPEEKIVKKWRLQPGKMLLIDLEKG
                     RIISDDEIKSEIAARHPYKTWLSNTQLILEDLKPVEPRALRRDVSLLDRQQAFGYTQE
                     DLKLLMSPMATTGQEAVGSMGTDTPIAAMSSRSSMLYTYFKQNFAQVTNPPIDPIREE
                     LVMSLVSFIGPRPNIFDLVGSSRRKRLEVRQPILTNGDLEKIRSIGHTEDRFDTKTID
                     ITYNASEGVAGMQGAVERLCERAEAAVAGGYNIIILSDRQIGPDRIEIPTLLATAAVH
                     HHLIRKGLRTSVGLVVESGEPREVHHFCCLAGYGAEAINPYLAFDTLADMHKNGAFPP
                     EVDAHEVVTRYIKSIGKGILKVMSKMGISTYQSYCGAQIFDAIGLRSEFVDKYFTGTA
                     TLIEGVGLDEIATETVNRHASAFGDDPVLRNALEVGGEYMYRMRGEAHVWTPDAVATL
                     QHAVRHGSPETFRQYSREVDSETARAQTIRGLFKIRTADETGRKKVSLDEVMPAAEIV
                     KRFSTGAMSFGSISREAHTTLARAMNAIGGKSNTGEGGEEPDRYLPLPGGGANPERSA
                     IKQVASGRFGVTAEYLVNSDMIQIKVAQGAKPGEGGQLPGHKVDATIAKVRHSTPGVG
                     LISPPPHHDIYSIEDLAQLIYDLKNVNPAADVSVKLVSEVGVGTVAAGVAKARADHIT
                     ISGYDGGTGASPMTSLKHAGSPWEMGLAETHQTLVLNGLRSRVALQVDGGLRTGRDVV
                     IGALLGADEFGFATAPLIAAGCIMMRKCHLNTCPVGVA"
     gene            4101..5153
                     /locus_tag="VGM46_08665"
     CDS             4101..5153
                     /locus_tag="VGM46_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HEY4192707.1"
                     /translation="MFFASDNWAGAHPKIAAGLAANAAGFSAAYGDGDLDKAVYRRFS
                     EIFEREVAVFFVATGTAANSLSLAAYNKTGGISLCHRESHVVEDECGAPEFFMGGSRL
                     LPIDGALGRIDASRLENALQRLAPEIVHTGRPAAVTITQSTEIGTVYSLDDIDEISGL
                     AKRHGLPLHMDGARFANAMVSLDTTPAEMTWKRGVDILSFGGTKNGCWCAEAVVLFDL
                     DRAKEMTFLRKRAAQLFSKSRFIAAQFEAYFKDGLWLETARHANAMAAKLAAAIRNAP
                     AARLAWQPQANEVFAVIKKADAQRIQAAGAAFYDWHQPTGFTDPLTEDEALYRFVTSF
                     ATTADDIDRLGSLLAK"
BASE COUNT          940 a         1680 c         1573 g          991 t
ORIGIN      
        1 ggcgacgccc accgggcagg tgttcaggtg gcacttgcgc atcatgatac agccggccgc
       61 gatcagcggc gcggtggcaa agccgaactc gtcggcgccc agcagcgcgc cgatcaccac
      121 gtcgcggccc gtgcgcaggc cgccatcgac ctgcaacgca acgcggctgc gcaggccgtt
      181 cagcaccagc gtctggtgcg tctcggcaag gcccatttcc cacgggctgc cggcatgttt
      241 cagcgacgtc atcggcgatg cgccggtgcc gccatcatag cccgaaatgg tgatgtggtc
      301 ggcgcgcgcc ttggcaacgc cggccgcaac cgtgccgacg ccgacctccg acaccagctt
      361 gacggaaacg tccgccgccg gattgacgtt cttcagatcg tagatcagct gcgccagatc
      421 ctcgatcgaa tagatgtcgt gatgcggcgg cggcgagatc agcccgacgc ccggcgtgga
      481 gtggcggacc ttggcaatcg tcgcatcgac cttgtggccg ggcaattgcc cgccctcgcc
      541 gggcttcgcg ccctgcgcca ccttgatctg gatcatgtcg gaattgacca gatattccgc
      601 cgtgacgcca aagcggccgg aagccacctg cttgatcgcg gagcgctcgg ggttggcccc
      661 gccgcccggc aacggcagat agcggtccgg ctcctcgccg ccctcgcccg tgttcgactt
      721 gccgccgatg gcgttcatcg cccgcgccag cgtcgtatgc gcctcgcgcg agatggagcc
      781 gaacgacatg gcgcccgtcg agaagcgctt gacgatttcc gcggccggca tcacctcgtc
      841 cagcgaaacc ttcttgcggc cggtctcgtc ggcggtgcgt atcttgaaca ggccgcgtat
      901 ggtctgcgcc cgcgccgtct cgctgtcgac ttcgcgcgaa tactgcctga acgtctccgg
      961 tgaaccatgc cgcacggcgt gttgcagcgt cgccaccgca tccggcgtcc acacatgcgc
     1021 ttcgccgcgc atgcggtaca tatattcgcc gccgacttcg agcgcattgc gcaacaccgg
     1081 atcgtcgccg aacgccgagg cgtggcgatt gaccgtttcc gtcgcgatct cgtccagccc
     1141 gacgccctcg atgagcgtgg ccgtgccggt gaagtacttg tcgacgaact ccgaccgcaa
     1201 gccgatggcg tcgaatatct gcgcgccgca ataggactgg taggtcgaga tgcccatctt
     1261 ggacatgacc ttcaggatgc ccttgccgat ggacttgatg tagcgcgtca ccacctcgtg
     1321 cgcgtccacc tccggcggga aagcgccgtt cttgtgcatg tcggccagcg tgtcgaaggc
     1381 gagatagggg ttgatcgcct ccgcgccata gccggccaga cagcagaaat gatgaacctc
     1441 gcgcggctcg ccggattcca cgaccaggcc gaccgaggtg cgcaagccct tgcggatcag
     1501 atgatgatgc acggcggccg tcgccaaaag cgtcggaatc tcgatgcggt ccggcccgat
     1561 ctggcgatcg gacaggatga tgatgttgta gccgccggca accgccgcct ccgcccgctc
     1621 gcaaagccgc tcgacagccc cctgcatccc cgccacgcct tcgctggcgt tataggtgat
     1681 gtcgatggtc ttggtatcga aacggtcttc ggtgtggccg atggagcgga ttttttcgag
     1741 gtcgccattg gtcaggatcg gctggcgtac ctccaaccgc ttgcggcgcg acgacccgac
     1801 gaggtcaaaa atgttcgggc gcggcccgat gaaggaaacg aggctcatca ccaattcctc
     1861 gcggatcggg tcgatcggcg ggttggtgac ctgcgcgaaa ttctgcttga aataggtgta
     1921 gagcatcgac gaccggctcg acatcgccgc aatcggcgtg tcggtgccca tcgaaccgac
     1981 ggcttcctgc ccggttgtcg ccatcggcga catcaacagc ttcaaatcct cctgcgtgta
     2041 gccgaaagcc tgctggcgat cgagcagcga aacatctcgg cgcagcgcgc gcggctccac
     2101 cggcttcaaa tcctcaagga tgagctgcgt gttggaaagc caggtcttgt aggggtggcg
     2161 cgccgcgatt tccgacttga tttcatcgtc ggagatgatg cggccctttt caaggtcgat
     2221 cagcagcatc tttcccggct gcaagcgcca cttcttgacg atcttttctt ccggcacatc
     2281 gagcacgccg gcctccgacg ccatgatgac gcggtcgtcg tcggtgacga tatagcgggc
     2341 cgggcgaagc ccgttgcggt cgagcgtcgc gccaatctgg cggccgtcgg taaaggcgac
     2401 cgcggccggc ccgtcccatg gctccatcag ggcggcgtgg tattcataga atgccttgcg
     2461 gtccgcgccc atcagcttgt tgccggccca tgcctcgggg atcagcatca tcatggaatg
     2521 ggcaaggctg tagccgccct ggaacaggaa ttcgagcgcg ttgtcgaaac aggccgtatc
     2581 cgactgccct tcataggaaa tcggccacag cttggagatg ttgttgccga acagctcgct
     2641 gtcgacggaa gcctgacgcg ccgccatcca gttgttgttg ccgcgaaccg tgttgatctc
     2701 gccattatgc gcgaccatcc ggtagggatg cgccaacttc cacgagggga aggtgttggt
     2761 ggaaaaacgc tggtgaacga ggatcagcgc cgtctcgaag cgcggatcgg caaggtcctt
     2821 gtagtaggcg ccgacctgat aggccaggaa catgcccttg tagacgatgg tgcgcgccga
     2881 cagcgacacg caataggcgc cgatatcctt gttgtcgttc tctgcataga tgcgcccgga
     2941 aatgaccttg cgcagcaggt aaagccgcgc ttcatattcc tcgtcatcgt cgatatcggg
     3001 cgtgcgcccg atgaagacct gacggtgaaa tggctcggcc gccgcgattt ccggggactt
     3061 ggatagcgat gaattgtcga ccggcacatc gcggaagccg atcagcggca ggccttcgga
     3121 ctgcgccgat tcacggatga tctcgtcgat atgcgtgcgc agcgcctcgt cctgcggcat
     3181 gaaccagtgg ccaacgccat attggccgac cggcggtagt tcgatgtcct gcctggccag
     3241 ctcttcacgg aaaaaccggt ccggaatctg caccagcacg ccggcgccgt cgcccatcaa
     3301 cggatcggcg ccgacggcgc cgcgatgggt gaggttttcg agcatggcga ggccgtccac
     3361 gacgatgccg tgcgactttt cgttcttcat gttggcaatg aagccgacgc cgcagccatc
     3421 atgctcattg cgcggatgat aaagaccctg cggggccatg ccggtcgtgc tgcgcagcgc
     3481 ggcacgtttg acggatgccg cgttcgtctg cgcggcctgg ccattcgtcg cggcaagcgt
     3541 caactcggtc atcgtctcat cctttccggt cggccgctgg attagtcgga aagcgctggc
     3601 tttccccgaa actgcggcac tattgcaaac ccattgtcta gatgatcttc gcgccaacgg
     3661 gccgaacgtc ctgccggctt ttcccggcgt cggttcgacc gcttgcgcgt tgttcccgac
     3721 aaaccgccag gcgcacagag tacgccattg ccgcccatcg gggagattaa aataagacag
     3781 caatgctgtc ctaaatttta tggcagaaac ctttggccca cacaagaccg attcagaaaa
     3841 aacttggaac aaggacgcag caaagttgcg ctaattgcac cacgcacgaa actatttgtc
     3901 gtcaggccgt tcattcagcg ccctgcccct tactcagcgc ccgctgcgca ttcagcggcc
     3961 aaccccttat tcgtcatcag ccctttaacc ttggccgcat gcctctttca tttttgccgc
     4021 cgctggctct tgcaaccttg atggcaagcc cggcaatgtc gcgccgtttc cggcaatcag
     4081 cagttcaggc aggcctattc atgtttttcg cttccgacaa ttgggccggc gcccacccga
     4141 agatcgcggc cggcctggcg gccaacgccg ccggcttttc cgctgcctat ggcgacggcg
     4201 acctcgacaa agccgtctat cggcgcttca gcgagatctt cgaacgcgaa gtcgcggtgt
     4261 ttttcgtggc cacaggaacg gcggccaatt cgctgtcgct ggcggcctac aacaagaccg
     4321 gcggcatttc cctttgccat cgcgaatcgc atgtcgtcga agacgagtgc ggcgcgccgg
     4381 aattcttcat gggcggctcc cgccttctgc cgatcgacgg cgcgcttggg cgcatcgatg
     4441 cgagcaggct ggaaaacgcc ttgcagcgcc tcgcgcccga aatcgtgcat accggccggc
     4501 cggcggcggt gacgatcacc caatccacgg aaatcggaac cgtctatagc ctggacgaca
     4561 tcgacgagat ttcaggcctc gccaaacgcc acggcctgcc cctgcacatg gacggcgcgc
     4621 gctttgccaa cgcaatggtt tcgctcgata cgacgccggc cgaaatgacc tggaagcgcg
     4681 gtgttgacat cctttccttt ggcggcacca aaaacggctg ctggtgcgcg gaagccgtgg
     4741 tattgttcga cctggacagg gccaaggaaa tgacattcct gcgcaagcgc gccgcccagc
     4801 tcttttccaa gtcgcgtttt atcgccgccc agttcgaagc ttatttcaag gacgggctat
     4861 ggctggagac cgcccgccac gccaacgcca tggccgcgaa actggcggcc gccatccgca
     4921 atgccccggc cgcacggctc gcctggcaac cgcaggcaaa cgaagtgttt gccgtcataa
     4981 agaaggccga cgcgcagcga attcaggcgg cgggcgccgc gttttacgat tggcaccagc
     5041 cgaccggttt caccgatccg ctgactgagg atgaggcgct ctaccgtttc gtcaccagtt
     5101 tcgcgaccac ggccgacgat atcgacaggc tgggcagcct tctcgccaaa tgaaagacgg
     5161 cgcttttcag cgccgccttt acta
//
LOCUS       DASYCS010000272         7258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2533287,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000272 DASYCS010000000
VERSION     DASYCS010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 7258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7258
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2533287"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3526
                     /locus_tag="VGM46_08670"
     CDS             <1..3526
                     /locus_tag="VGM46_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010915531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein ndvB"
                     /protein_id="HEY4192708.1"
                     /translation="TDRHIEVTSFAELALAPETSDNAHPAFSKMFVETEIARNNGVIF
                     AARRKRDPGEPDIAAAHFVTDTSGLARDTEAETDRRAFIGRGRTIADAAAFDAGAKLG
                     GGSGFTLDPVASLRRQVRVAANKKISLTFWTVVGANRKEMEDAVARLDHPESFSRQAM
                     LAWTRSQVQTRHLGLSLADAANVQKLARYLLYPDPFLRVPAETIAAGLGKQSSLWPTS
                     ISGDFPIFVVRIADIADIEIVAQALRFQEYMRARGMVADLVIVNEQASSYVQDLQQAI
                     ESLCENSRARGTELGPRQHIFTVRRDLMDEATYKTLLAVARVVLHTRNGLIFDQIERA
                     EANALQARDTLVPQRALAASENLPAAKSPASIEDKPANGKGLANWNGFGGFDGDGRHY
                     VVRLTGRRTTPQPWINVVSNETFGFHTSAEGAAFTWSRNSRDYQLTPWSNDPVTNRPG
                     EGIYIHDLESGEAFAPIAAVVRDPKMTYEAWHGQGFSTFRSARGSLTMDLTHVVDPVD
                     PVKISRLRIQNTGSSPARLRVYAYAEWVLGGHRSRTASTIIPSQDAATGALLAQNPYG
                     LDYGDRIAFLGSDGVPDSVTADRLEFLGRHGSSVAPQSVLFGNALSGNIEAGGDPCAA
                     IARDIEIPAGGDVTLLWLMGDASSAAEASDLMQRHRAKDFDQRLADNESQWRGFLDTI
                     QVETPDKSLDAMVNHWLPYQALACRIRARSAFYQASGAFGFRDQLQDTMALLTHDPSL
                     GREQILNAARRQFAEGDVQHWWLPRTGAGVRTIISDDVVWLAHAVARYISVSDDKAIL
                     KEKLPFIEGQALAEGEHDAFFVPVVSEKTASLYEHCARAIDLAIQRSSPNGLPLMLGG
                     DWNDGMNRVGEGGKGESVWLGWFLLATLGDFAPIAKAEGDAKRAQAWEKHARTLKRAL
                     ESTAWDGQWYRRGSFDDGTPLGSHNSEECRIDSIAQSWSVLSGEGDPARSRTAMEQAM
                     ELLVDDENKLIKLFTPPFSNTPHDPGYIKAYPPGVRENGGQYTHAATWFVIALAEMGR
                     TDDAYRCFSMLNPVNHALDETAAERYRVEPYVVAADIYAEQGKGGRGGWTWYTGSAGW
                     LYRAAVEGILGIHQHGKRVEIAPKLPSSWDGYSATLKILGGECKIRVLRDKTAKTVTI
                     EKDGVKVKGLSFEPKAGATIELVVKIPK"
     gene            3773..5551
                     /locus_tag="VGM46_08675"
     CDS             3773..5551
                     /locus_tag="VGM46_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015441 - ABC-type beta-glucan transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015775 - beta-glucan transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan ABC transporter ATP-binding protein/
                     permease"
                     /protein_id="HEY4192709.1"
                     /translation="MSLLQIYWRALGYLASDKKRVAFICAANVTLAIITILEPILFGR
                     VIDAMSGHQDVVPTLALWAGLGAFNIIAFVWVARGADRFAHARRGELLCESFERVITM
                     PLSWHQQRGTSNSLHTLLRAIESLFSLWLEFMRQHLSAAVALCLLVPTALNLDVRMAT
                     VLLVLGAVYVGVGRLVMRRTKAGQANVERHYHQVFGHVTDTVSNVSVLQSYNRIGLEA
                     ETLRQHVRNLLSAQNPVLDWWAIASALQRLSSTISMMVVLLIGAYLVTRGELRIGDVV
                     AFTGFATVLIARLDQISAFANQISDARAKLEEFYRLEDSAVDAAEPEGLRDLGAVSGH
                     VRFENVSFEFANSGQGVHDVSFEVQAGQTIAIVGPTGAGKTTVINLLQRVFSPSSGRI
                     LIDGIDTRTVTRKSLRHSIATVFQDAGLLNRSIEDNIRVGRAEASYDEIHAAAAAAAA
                     QDFILSKGSGYDTLVGERGGQLSGGERQRIAIARAVLKNAPILVLDEATSALDVETED
                     RVKEAIDVLRRDRTTFIIAHRLTTVRDADLVVFMDKGRVVEMGGFTELSLRNGRFASL
                     LRAGGLLNDEEVRRLSRISSVKEAAA"
     gene            5640..6404
                     /locus_tag="VGM46_08680"
     CDS             5640..6404
                     /locus_tag="VGM46_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease"
                     /protein_id="HEY4192710.1"
                     /translation="MSDASERPAIWTDFANPTRFVGLADRLVPWLAGISAVILAIGLY
                     MSFAAPEDFQQGITVRIMYIHVPFAWLSMLCYSVMAVSALGTLVWRHPLADVALKAAA
                     PIGAVFTALALITGSIWGKPMWGTWWVWDARLTSVFVLFLMYLGIMALTRALDDVGRA
                     SWAAAIVTLVGFINIPIIKFSVDWWNTLHQPASVFRLDGPTIDPSLLWPLLVMAVGFT
                     FLFFALHLSAIRTEIWRRRVTAMRRIAARNAERLPA"
     gene            complement(6406..>7258)
                     /locus_tag="VGM46_08685"
     CDS             complement(6406..>7258)
                     /locus_tag="VGM46_08685"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009364274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEY4192711.1"
                     /translation="EVMADAGLGDIFLPYNILGKTKLERLLALHRRVTLSVTVDNAET
                     LAGLTDTFADAGHRLQVLVECDTGMGRCGVQSAAEALALARDIDAANGLSFGGLMTFP
                     APGRPDEAEDWLRGAKHALLHAGLECLRISSGGTPDMWRAAEETVITEYRPGTYIYMD
                     RYQVAHGAATLDDCALTVLATVVSHPTATRAIIDAGSKALSSDTMGLSDFGEVLGMPD
                     ATVNGLSEEHGKLLLCGSANLRIGERVRIVPDHCCVVVNLFDQVHLISGEEVVKTLPV
                     AARGLMA"
BASE COUNT         1284 a         2331 c         2224 g         1419 t
ORIGIN      
        1 taccgatcgt catatcgagg tgacttcctt tgccgagctg gcgctggcgc ccgaaacctc
       61 cgacaatgcg caccctgcat tctcgaaaat gttcgtcgag acggaaatcg cgcgcaataa
      121 cggcgtcatc ttcgccgcgc ggcgcaagcg cgatccgggc gaaccggaca tcgcggcggc
      181 gcatttcgtc accgacacat ccggtcttgc gcgcgatacc gaggcggaaa ccgaccggcg
      241 cgcctttatc gggcgaggac gcaccatcgc cgatgccgcc gccttcgacg cgggcgcaaa
      301 gctcggcggc ggttccggct tcaccctcga tccggtggcg tcgctgcgcc gtcaggttcg
      361 cgtggccgcc aacaagaaaa tttcgctgac gttctggacg gttgtcggcg ccaaccgcaa
      421 ggaaatggaa gacgcggtgg caaggctcga ccaccccgag agcttctcgc gccaggccat
      481 gcttgcctgg acacgctcgc aggtgcagac gcgccatctc ggcctcagcc tggccgatgc
      541 cgccaatgtg cagaaactgg cgcgctatct tctttatccc gatccattcc tgcgcgtgcc
      601 ggcggagacg attgccgctg gcctgggcaa gcaatccagt ctgtggccga ccagcatatc
      661 tggcgatttc ccgattttcg tcgtcaggat cgccgatatc gccgatatcg agatcgtcgc
      721 ccaagccctg cgcttccagg aatatatgcg tgctcgcggc atggtggcag acctcgtcat
      781 cgtcaacgag caggcttctt cttacgtgca agatttgcag caggccatcg agtccctgtg
      841 cgaaaacagc cgcgcgcgtg gcaccgagct tggaccgcgc cagcacattt tcacggtgcg
      901 ccgcgacctg atggacgagg ccacttacaa gacgctgctc gcggtggcgc gtgtcgtgct
      961 gcatacacgc aatggcctga tcttcgacca gatcgagcgc gccgaagcca atgccttgca
     1021 ggccagggac acgctggttc cgcaacgcgc tttggcggcg agcgaaaatt tgccagccgc
     1081 caaatcgcct gcatcgatcg aagacaaacc cgcgaacggc aagggcctgg ccaactggaa
     1141 cggctttggc ggcttcgatg gcgacgggcg gcactatgtc gttcgcctga cgggccggcg
     1201 cacgacgccg caaccgtgga tcaacgtggt ttcgaatgaa accttcggct tccatacctc
     1261 cgccgaaggc gccgccttta cctggagccg caacagtcgc gactatcagt tgacgccgtg
     1321 gtcgaacgac ccggtcacca accggccggg cgaaggcatc tacatccacg atcttgaaag
     1381 cggcgaagcg ttcgcgccga tagccgccgt ggttcgcgac ccgaaaatga cctatgaggc
     1441 gtggcacggc cagggcttct cgaccttcag gtccgcgcgc ggttccttga cgatggatct
     1501 gacccatgtg gtcgatccgg tcgatccggt gaagatttcc cggctgcgca ttcaaaatac
     1561 aggatcgtcg cccgccaggt tgcgggtcta cgcctatgcc gaatgggtgc tgggcggcca
     1621 ccgctcgcgc accgcatcga ccatcattcc ctcgcaggat gcggcaacgg gcgcgcttct
     1681 ggcgcagaac ccgtacggcc tcgactatgg cgaccggatc gcctttctcg gcagcgacgg
     1741 cgtaccggac tcggtcaccg ccgaccgcct ggaattcctt gggcggcacg gcagcagcgt
     1801 ggccccgcag tcggtgcttt tcggcaatgc tttgtctgga aacatcgaag cgggcggcga
     1861 tccatgcgcc gcgattgcgc gcgatatcga gataccggct ggcggcgatg tcacgcttct
     1921 atggttgatg ggcgacgctt cgtcggccgc tgaagccagc gatctgatgc agcgccaccg
     1981 cgccaaggat ttcgaccagc gccttgccga caatgaaagc cagtggcgcg gtttcctcga
     2041 tacgatccag gtcgagacgc cggacaagtc gctggacgcc atggtcaacc actggctgcc
     2101 ctaccaggcg cttgcctgcc gcatccgtgc gcgttcggcc ttctatcagg ccagcggcgc
     2161 gttcggcttc cgcgatcagt tgcaggacac gatggccttg ctcacgcacg atccatcgct
     2221 gggccgcgag cagatactca atgcggcccg ccggcagttc gccgagggcg acgtccagca
     2281 ttggtggctg ccgcgcaccg gcgccggcgt gcgcacgatc atctccgacg atgtcgtgtg
     2341 gctggcgcat gccgtcgccc gctatatctc ggtcagcgac gacaaggcga tcctcaagga
     2401 gaaactgccg ttcatcgaag gacaggcgct ggctgaaggc gagcacgacg ccttcttcgt
     2461 gcccgtcgtt tcggaaaaga ccgcttcgct ctacgagcac tgcgcccgcg ccatcgatct
     2521 ggccatccag cgcagcagcc ccaatggctt gccgctgatg ctgggcggcg actggaacga
     2581 cggcatgaac cgcgtcggcg aaggcggcaa gggcgagagc gtctggctgg gctggttcct
     2641 gctggcgacg ctcggcgatt ttgcgcccat cgccaaggcc gaaggcgacg ccaagcgcgc
     2701 gcaggcatgg gaaaagcatg cccgtacatt gaagcgcgcg ctggaaagca ccgcctggga
     2761 cggtcaatgg tatcgtcgcg gcagcttcga tgacggcacg ccgcttggct cgcacaattc
     2821 cgaggaatgc cggatcgatt cgatcgcgca gtcgtggagc gtgctatcgg gcgagggcga
     2881 ccccgcgcgt tcgcgcacgg cgatggaaca ggcgatggaa ttgctggtcg atgacgagaa
     2941 caagcttatc aagcttttca cgccgccctt ctcgaacact ccgcacgatc cgggctacat
     3001 caaggcctat ccgcccggcg tccgcgagaa tggcggccag tacacccatg ccgctacctg
     3061 gttcgtgata gcgctggccg aaatggggcg cacggacgac gcctatcgct gcttctcgat
     3121 gctcaatccg gtcaatcatg cgctggatga gaccgctgcc gagcgttacc gcgtcgagcc
     3181 atatgtcgtt gccgccgaca tttatgccga gcaaggcaag ggcgggcgcg gcggctggac
     3241 ctggtacacg ggttctgccg gctggctgta cagggcggcc gtcgaaggca ttctcggcat
     3301 tcaccagcat ggcaagcggg tggagattgc gccaaagctt ccaagtagtt gggatggtta
     3361 ttcggctacc ctcaagatat tgggcggcga atgcaagata cgcgtcttgc gcgacaagac
     3421 ggccaagacg gtaaccattg aaaaggatgg cgtgaaggtg aagggcttgt ctttcgagcc
     3481 gaaggccggc gccacaatcg agctggttgt aaaaatcccg aagtaatcgg cgctcaccct
     3541 cgactgcata agataaaggc agcggacccc gctgccttta ttttgccgca cagctgacat
     3601 tttggctgtc ttctcaaacc gttagacgat cacgccagat ttcgggccgt tgtcatgttt
     3661 tgttcgcgga atcggaacag aagggtccca aaggcattgt tttgacacgc ttcaatcgat
     3721 acgtctttgg cacttcaata accctgcaca atgagtttgg tggagtagtc cagtgtccct
     3781 tctgcaaatc tactggagag cactcggtta tcttgcttcg gacaagaagc gcgtcgcgtt
     3841 tatttgcgcg gctaatgtaa cgcttgccat catcaccatt ctcgaaccca ttctgttcgg
     3901 gcgcgttatc gacgccatgt ccggccatca ggatgtcgtg ccgacgctgg cgctgtgggc
     3961 gggccttggc gctttcaaca tcatcgcttt cgtctgggtg gcgcgtggcg ccgaccgttt
     4021 cgcccatgca aggcgcggcg agcttctttg cgaatccttc gagcgcgtca tcaccatgcc
     4081 gctgtcctgg catcaacagc gcggcacgtc caattcgctc cataccttgc tgcgcgccat
     4141 cgaatcgctg ttcagcctgt ggctcgaatt catgcgccag cacctttcgg cggcggtcgc
     4201 gctttgcctt ctggttccca ccgcgcttaa cctcgatgtc cgcatggcga cggttcttct
     4261 cgtcctcggc gcggtctatg tcggcgtcgg ccggctggtc atgcgccgga cgaaggctgg
     4321 ccaggccaat gtcgagcgcc attatcacca ggtcttcggc catgtgacgg atacggtcag
     4381 caacgtttcg gtgttgcaga gctataaccg catcggcctc gaagccgaga cgctgcgtca
     4441 gcacgtccgc aacctgctca gcgcccagaa cccggtgctt gactggtggg ctattgccag
     4501 cgcgctgcaa cgcctgtcgt cgaccatttc gatgatggtg gtgctgctga tcggcgccta
     4561 tctggtcacg cgcggcgaac tgcgcatcgg cgatgtcgtc gcctttaccg gctttgccac
     4621 cgtgctcatc gcccggctcg accagatttc ggctttcgcc aaccagattt ccgacgcccg
     4681 cgccaagctt gaggaattct accggctgga ggattcggcc gtcgacgctg ccgagccgga
     4741 aggcctgcgc gacctcggcg cggtgagcgg gcatgtgcgc ttcgaaaatg tgtccttcga
     4801 attcgccaat tccgggcagg gcgtgcatga cgtttcgttc gaggtccagg ctgggcagac
     4861 cattgccatc gtcggcccga caggcgccgg caagacgacg gtcatcaacc tgttgcagcg
     4921 cgtcttctcg cccagcagcg gccgcatcct catcgacggc atcgatacgc gcacggtgac
     4981 ccgcaagtcg ctgcgccatt ccatcgccac cgtcttccag gatgccggcc tgctcaaccg
     5041 ttcgatcgag gacaatatcc gcgtcggtcg cgccgaggct tcctatgacg agattcatgc
     5101 tgccgccgct gctgccgctg cgcaggattt catcctgtcc aagggcagcg gctacgacac
     5161 cctggtcggc gaacgcggcg gccagctttc cggcggcgag cgccagcgca tcgccatcgc
     5221 ccgtgccgtt ctgaagaacg cccccatcct cgtgcttgac gaggcgacca gcgcgcttga
     5281 cgtggaaact gaagaccgtg tcaaggaagc catcgacgtg cttcgccgcg accgcaccac
     5341 cttcatcatc gcccaccgcc tgaccacagt tcgcgacgcc gatctggtcg tgttcatgga
     5401 caagggcagg gtggtcgaga tgggcggctt taccgaacta tccctgcgca atggccggtt
     5461 tgcttcgctg ttgcgcgccg gtggcctgct caacgatgag gaagtccgtc gcctgagccg
     5521 catttcgtcg gtcaaggaag ccgccgcctg accgaggcgc ggcgctcttg ccgatttcgc
     5581 tgccctgcga catcggcgct cgattgccgc aacggcttcc tcggtctatg taacattgca
     5641 tgagcgacgc cagcgaacgc cccgctatct ggaccgattt cgccaatccg acacgctttg
     5701 tcgggctggc ggatcggctc gtgccatggc tggccggcat ttcggcggtc attctggcta
     5761 tcggccttta tatgagcttt gcggcgccgg aagatttcca gcagggcatc accgttcgca
     5821 tcatgtatat ccatgtgccg ttcgcatggc tttcaatgct gtgctactcg gtgatggcgg
     5881 tttcggcgct cggaaccctg gtgtggcgtc atccgctggc cgatgtcgcg ctcaaagcag
     5941 ccgcccccat tggcgccgtc ttcacggcgc tggcgctgat caccggctcc atctggggca
     6001 agccgatgtg gggaacatgg tgggtgtggg atgcgcggct gacatccgtg ttcgtgcttt
     6061 tcctgatgta tctcggcatc atggcgctga cgcgcgcgct ggacgatgtt ggccgtgcct
     6121 catgggcggc cgcgatcgtc acgctggtcg gcttcatcaa catcccgatc atcaaattct
     6181 cggtcgactg gtggaacacg ctgcatcagc cggcctcggt gttccggctc gatggcccga
     6241 ccatcgatcc cagcctgcta tggccgctgc tggtcatggc ggtcggcttt accttcctgt
     6301 tcttcgcgct tcacctgagc gcgatccgaa cggagatttg gcggcggcgg gtgaccgcca
     6361 tgcgccggat agcggcgcgc aacgccgagc ggctgccggc ttaagtcaag ccatcagccc
     6421 gcgcgccgcc accggcagcg tcttgaccac ttcctcgcct gaaatcaggt gaacctggtc
     6481 gaatagattg accacgacac agcaatggtc gggaacgatg cgcacccgct cgccgatgcg
     6541 aaggttggcg ctgccgcaaa gcaacagctt gccatgttcc tcgctaaggc cgttgacggt
     6601 tgcgtccggc atgcccagca cctcgccgaa atcggaaagc cccatggtgt cactggacag
     6661 cgctttcgag ccggcatcga tgatcgcccg cgtcgccgtt ggatggctga ccacggttgc
     6721 cagcacggta agcgcgcagt cgtccaacgt cgccgcgcca tgggcgacct gatagcggtc
     6781 catgtaaata taggtgccgg gccgatattc ggtgatgacg gtttcctcgg ccgcgcgcca
     6841 catatcggga gtaccgccgc tggaaatgcg caggcattca agcccggcat gcagaagcgc
     6901 atgctttgcg ccgcgcagcc agtcctccgc ctcgtcgggc cgtcccggcg ctggaaacgt
     6961 catcagcccg ccaaaggaca ggccatttgc ggcgtcgatg tcgcgcgcca gcgccaaagc
     7021 ttctgcggcc gactgcacgc cgcatcgacc catgccggta tcgcattcga ccagcacttg
     7081 cagcctgtgg ccggcatcgg caaaggtatc agttagcccg gcaagcgttt cggcattgtc
     7141 tacggtgacg gatagcgtga cgcgccgatg caaggccaga agccgctcca gcttcgtttt
     7201 gccgaggatg ttgtacggca ggaaaatatc gccaagcccg gcatccgcca tcacctcg
//
LOCUS       DASYCS010000273         3906 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1666696,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000273 DASYCS010000000
VERSION     DASYCS010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3906
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1666696"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VGM46_08690"
     CDS             <1..253
                     /locus_tag="VGM46_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008039340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEY4192712.1"
                     /translation="GKPENIIEKMVEGRLRKFYEEVVLLKQAFVLNPDVTVEQALKEA
                     EKEIGAPAKISAYLRFALGEGIEKEETDFAAEVAAAVKG"
     gene            382..1014
                     /locus_tag="VGM46_08695"
     CDS             382..1014
                     /locus_tag="VGM46_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013530072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEY4192713.1"
                     /translation="MPSHELLIAFFITTAIFAYIPGPAMLYAAAQTVAGGRSSGLMAA
                     LGIHLGCYVHVFAAAAGLSVLFHAVPVLYLAVKLGGAAYLIWLGVAMFREKDRGGADI
                     EPIRPKAGRRAFFESVTIEVLNPKTAIFFLAFLPQFIDLSAAFPVWLQFVILGTAVNL
                     MFSSADIVCVFLAGGVMDTIRRSSRAQRYMRKAGGAVLVGLGLRLALEKS"
     gene            1202..1924
                     /gene="pyrH"
                     /locus_tag="VGM46_08700"
     CDS             1202..1924
                     /gene="pyrH"
                     /locus_tag="VGM46_08700"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018428106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEY4192714.1"
                     /translation="MTAKPLYRRVLLKASGEALMGEQHFGIDVSVVDRIAADIADARA
                     LGIEVGVVIGGGNIFRGVAVASKGGDRVTGDHMGMLATVINSLALRTSLIKIGVDAVV
                     LSAIAMPELCESFSQRQATAYMDEGKVVIFAGGTGNPFFTTDSAAALRAAEIGADALF
                     KGTQVDGVYSADPKKDPNATRFERITHAEVINKGLSIMDTAAIALARENNIPIIVYSI
                     HEAGGLGEILRGGGRCTVVADA"
     gene            2007..2558
                     /gene="frr"
                     /locus_tag="VGM46_08705"
     CDS             2007..2558
                     /gene="frr"
                     /locus_tag="VGM46_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEY4192715.1"
                     /translation="MSGYDDLKRRMDGAIAAFKHDLASLRTGRASSNLLDAIQVQAYG
                     ATMPINQVANVSVPEPRMISVSVWDKAMVSAVDRAIRESNLGFNPIVDGTNLRIPLPE
                     LNEQRRKELVKISQTYAENARVAARHVRRDGMDSLKKAEKDGDISEDDQRKQSDQVQK
                     LTDETIGVIDSLLAEKQTEIMQV"
     gene            2579..3310
                     /locus_tag="VGM46_08710"
                     /pseudo
     CDS             2579..3310
                     /locus_tag="VGM46_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837945.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
     gene            3307..>3906
                     /locus_tag="VGM46_08715"
     CDS             3307..>3906
                     /locus_tag="VGM46_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEY4192716.1"
                     /translation="MSNLQLRIVSATVLAILVLGLTWLGGLPFRLLCAVISAAVFYEW
                     SRMSRPTPAAGLGLLPEALMAVFIVALVAGFSAQNLLLLLAVIVVAGAVFGPLQGRGQ
                     WEAGGLAYAGLSGLTLALLRDGDRSGLVAILFLFAVVWATDILAYFVGRAVGGPKLAP
                     SISPGKTRSGALGGAAGGVVAGVLLGVAIGAGNVAMLGVV"
BASE COUNT          666 a         1183 c         1270 g          787 t
ORIGIN      
        1 cggcaagccg gaaaacatca tcgagaagat ggtcgaaggc cgtctgcgca agttctacga
       61 ggaagtcgtg ctgttgaagc aggccttcgt gctcaatccc gacgtcaccg tcgagcaggc
      121 gctgaaggaa gcggagaagg agatcggcgc gcccgccaag atttccgcct atctgcgctt
      181 tgctctgggc gagggcatcg aaaaggaaga gaccgatttc gccgcggaag tggccgctgc
      241 ggtaaagggt tgaagccggt ttcgcttgcc gacgccggcg gcgcgctggc taagaattga
      301 acttcgaaag gaccgcgacg aaattccgtc gcggtctttt catttgaata gcgatgaaag
      361 ttcgccgtcc cggagtgagc catgccgtct catgaattgc tgatcgcgtt tttcattacc
      421 acggcgatct tcgcctatat tccaggaccg gccatgcttt atgccgcagc ccagacggtg
      481 gccggtggcc gctcatccgg gttgatggcg gcgctcggca ttcatctggg ctgttatgtc
      541 catgtctttg cggcggcggc cgggctttcc gtgctgtttc atgccgtgcc ggtgctgtat
      601 ctcgccgtca agcttggtgg cgccgcctat ctaatctggc tcggcgtggc gatgtttcgc
      661 gaaaaggatc gtggcggcgc ggacatcgaa ccgatccggc ccaaggccgg gcgtcgcgcg
      721 tttttcgaaa gcgtgacgat cgaagtgctt aatcccaaga cggcgatatt cttcctggct
      781 ttcctgccgc agtttatcga tttgtcggcg gcatttccgg tctggttgca gttcgtcatc
      841 ctcggtacgg cggtaaatct gatgttttcc tcggccgata tcgtctgcgt gtttctggcc
      901 ggcggcgtga tggacacaat ccggcgctca agccgcgcac agcgctacat gcgtaaggcc
      961 ggtggcgcgg tgcttgttgg acttggcctg cggcttgccc tggagaaatc ctgacagcct
     1021 gcccgccgga tcggaaagca gcgccgcttc aagcgcttcc ggtcatttca tggtgctttc
     1081 cacagggcgc accgcgtgac atgcgagcag ccttcgtgta tcgcacccaa agcattctgg
     1141 agcaggctgg ccgcaaggcg cgccgcccat acgcataatt cgcagcacga tgaggacata
     1201 gatgacagcc aagcctcttt accgacgtgt gttgctgaag gcgtcgggcg aggcgttgat
     1261 gggcgagcag catttcggca tcgacgtttc ggtggtcgac cgcatcgccg ccgatattgc
     1321 agatgcgcgc gcactgggca tcgaggttgg cgtcgtcatc ggcggcggca atatctttcg
     1381 cggtgtggcg gtggcttcca agggtggcga ccgcgtgacc ggcgaccata tgggcatgtt
     1441 ggcgaccgtc atcaattcgc tggcgctgcg cacctcgctg atcaagatcg gcgtcgatgc
     1501 ggtggtgctg tcggccatcg ccatgccgga actgtgcgaa agcttctcgc agcggcaggc
     1561 gaccgcctat atggacgagg gcaaggtggt gatcttcgcc ggcggcaccg gcaatccgtt
     1621 cttcaccacc gattcggcgg cggcgctgcg tgcggctgaa atcggcgccg acgcgctgtt
     1681 caagggcacg caggtcgatg gcgtctattc ggcagatccg aaaaaagacc cgaacgccac
     1741 ccgtttcgag cgcattaccc atgccgaagt gatcaataag ggcctttcca tcatggatac
     1801 ggccgcgatt gcccttgcgc gtgaaaacaa cattccgata atcgtctatt ccattcacga
     1861 ggcgggtggc ctaggcgaaa tcctgagagg cggcggccgc tgcacggtgg tggcggacgc
     1921 ctgaccgggc ttaactccgc cggtgttctt gaagccggcc ggaccctaac caaagacgcg
     1981 cgcccgagcg ggccgaggag gcaacgatga gcggttacga tgatcttaag cggcgcatgg
     2041 acggggcgat tgccgcgttc aagcacgacc tcgcttcgct gcgcaccggc cgcgcctcca
     2101 gtaacctcct cgatgccatt caggtgcagg cttacggcgc gacaatgccc atcaaccagg
     2161 tcgccaacgt ctcggtgccg gagccgcgca tgatctcggt ttccgtatgg gacaaggcga
     2221 tggtcagcgc ggtggaccgc gccatccgtg aatccaacct gggtttcaat ccgatcgtcg
     2281 atggcaccaa cctgcgcatt ccgctgcccg agcttaacga gcagcgccgc aaggaactgg
     2341 tcaagatttc gcagacctat gccgaaaacg cccgtgtcgc ggcgcgccat gtgcggcgtg
     2401 acggcatgga ttcgctgaaa aaggcggaaa aggacggcga tataagcgag gacgaccagc
     2461 gcaagcagtc cgatcaggtc cagaaactga cggacgagac gatcggcgtc atcgacagct
     2521 tgcttgccga gaagcaaacg gaaatcatgc aggtgtaggc ttcgccctcc atcggcccga
     2581 aagcaagaac atgacaaccc ccgcgcatgt cgcgatcatc atggacggaa acggtcgctg
     2641 ggccaaggcg cggggcctgc cgcggctcgc cgggcatcgc gccggcgtgg aggcgttgcg
     2701 caaggcggtc cgcgcggcgc ccgacctcgg tatttcctac ctgacggtct atgcgttttc
     2761 gtccgagaac tggtcgcggc cgaagtccga ggtcagcgac ctcatgggcc tgctgaagct
     2821 ttttatccga cgcgatctgg cggaattgca ccagaacggc gtgcgcgtgc gcatcgtcgg
     2881 cgaccggggc ggcctcaagc ccgacattac cgggctgctc gacgaggcgg aggccctgac
     2941 cgcctcgaac acggcgctca ccttggtcat cgctttcaat tacggcggac gcaacgaaat
     3001 cgtgcgcgct gcccgcaagc ttgctcaagc cgcgatgcgc ggcgaaatcg atgtcgacgc
     3061 gatcacgtcc gagacgttcg ccgcctcgct cgacacgacc gacattccag accccgaact
     3121 tttgatccgc accagcggcg agcttcgcct gtcgaatttc ctgctctggc aggcggccta
     3181 tagcgaactg gtgttcttgc aatgctattg gccggatttc agccgtgagc atcttgtcga
     3241 tgccttgcgc gacttcgccg gccgcgagcg tcgctttggc ggcgtcacgg ctcgggaagc
     3301 cgtgtcgtga gcaacctcca gcttcgcatc gtttcggcga ccgtgctggc gatcttggtg
     3361 cttgggctga cttggctggg cggcctgccg ttccgcctgc tttgcgccgt catttcagcc
     3421 gcggttttct atgaatggtc gcgcatgtcg cgtccgacgc ccgccgccgg gcttggcctg
     3481 ttgccagaag cgctgatggc ggtgttcatc gtggcgttgg ttgccgggtt ttccgcgcaa
     3541 aacctgctat tgctgctggc tgtaatcgtg gtcgcggggg cggtgttcgg cccgttgcag
     3601 ggcagggggc agtgggaagc gggcggcctc gcctatgcgg gtctgtccgg cttgacgctg
     3661 gcgctgctgc gcgacggcga ccgttccggc ctcgtcgcca tcctgtttct gtttgcggtg
     3721 gtgtgggcga ccgacatact cgcttatttc gtcggccgcg cggtcggcgg cccgaaactc
     3781 gcgccgtcca tttctcccgg caagacccga agcggcgcgc tcggcggcgc ggccggcggc
     3841 gtggtcgccg gcgttttgct cggagtagcc atcggcgccg gcaacgtcgc catgctgggc
     3901 gtggtc
//
LOCUS       DASYCS010000274         1557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3838057,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000274 DASYCS010000000
VERSION     DASYCS010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3838057"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VGM46_08720"
     CDS             <1..606
                     /locus_tag="VGM46_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010968300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY4192717.1"
                     /translation="SEFVTTLYGRNRKSAGEILWQGRQVAVDSEHDAIKLGMALAPES
                     RRDQGLCLNLPVGLNLNLPIYKRLSQNMLISGSEEKSHADRQIAGLRIKTPTRNVLAS
                     SLSGGNQQKIVIGKWLNHGAKLFIFDEPTVGVDVGTKAEIYRLFGQLLAGGAGIILIS
                     SYLPEVYELSDRLHVFRAGRLVASHGFRAASHEDVLTQAIG"
     gene            644..>1557
                     /locus_tag="VGM46_08725"
     CDS             644..>1557
                     /locus_tag="VGM46_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4192718.1"
                     /translation="MSTETVSIEKPKRNYNVLFGLTLLLLLAALWIALSLATPSFASG
                     INIANLLRQGSMIAILAVGQTFVIITGGIDLSVGAIVGFCTVIPALMINAGWPVWVAV
                     LATLLVGVGIGLFHGFGIVRMGLPPFIITLATLTSLRGIGLLMTNGNSISIRSDAFQE
                     FSRNSFLGVPNLFWMVILVGVPSYIFLHHSRWGRYLFSVGSNAEASRLSGVNVPRTIY
                     MAYILSGLCAAFVGVLLASRIGIGNPTQAEGWELQAIASSVIGGTSLFGAIGSVHGPL
                     LGAFILATINNGANLLNVNSFWQRVITGA"
BASE COUNT          268 a          521 c          450 g          318 t
ORIGIN      
        1 agcgaattcg tcaccacgct ctatggccgc aaccgcaagt cggcgggcga aatcctttgg
       61 caaggcaggc aggtcgcggt tgattccgag cacgacgcca tcaagcttgg catggcgctg
      121 gcacccgaaa gccgccgcga ccagggcctc tgcctcaacc tgccggtcgg cctcaacctg
      181 aatttgccga tctataagcg gctttcgcaa aacatgctga tctccggcag cgaggagaaa
      241 tcccacgccg accgccagat cgccggcctg cgcatcaaga cgcccacccg caacgtgctg
      301 gcatccagcc tgtcgggggg caaccagcag aagatcgtca tcggcaaatg gctgaaccac
      361 ggcgccaagc tgttcatctt cgacgaaccg acggtcggcg tcgatgtcgg caccaaggcc
      421 gagatttacc gcctgttcgg tcagttgctg gcaggcgggg cgggcatcat cctgatctcg
      481 tcctacctgc cggaagtcta tgaactatcc gacaggttgc atgtgttccg cgcggggcgg
      541 ctggtcgcca gccacggctt ccgcgcagcc agccacgaag acgtgttgac gcaggccatc
      601 ggctgacgca ggcgcgaata acaataaaaa cggggagcga aagatgagca ccgaaaccgt
      661 atccatcgaa aagccgaagc ggaactacaa cgtgctgttc ggcctgacgc tgcttttgct
      721 tctggcggcg ttgtggatcg ccctttcgct ggcgacgccc agcttcgcct ccggcatcaa
      781 catcgccaat cttctgcgcc agggatcgat gatcgccatc ctcgccgtcg gccagacatt
      841 cgtcatcatc accggcggca tcgacctttc agtcggcgcc atcgttggct tctgcaccgt
      901 tatccccgcc ttgatgatca acgcgggctg gcccgtctgg gtcgccgtgc tggccacgct
      961 gctggttggc gtcggcatcg gccttttcca tggtttcggc atcgtgcgga tgggcctgcc
     1021 ccccttcatc atcacgctgg ccaccttgac ctcgctgcgc ggcatcggct tgctgatgac
     1081 caacggcaat tcgatttcga tccgaagcga cgccttccag gaattttccc gcaattcgtt
     1141 tctcggcgtg cccaacctgt tctggatggt gatcctggtc ggcgtgcctt cctatatctt
     1201 ccttcatcac agccgctggg gccgctatct gttttcggtc ggctcgaatg ccgaggcatc
     1261 gcgcctgtcg ggcgtcaacg tgccgcgcac catctacatg gcctatatac tttcgggcct
     1321 ctgcgccgcc tttgtcggcg tgctgctggc ctcgcgcatc ggcatcggca acccgacgca
     1381 ggcggagggc tgggagttgc aggccatcgc ctcgtcggtg atcggcggca cctcgctgtt
     1441 cggcgccatc ggctcggtac acggcccgct gcttggcgcc ttcatcctcg ccaccatcaa
     1501 caatggcgcg aacctcttga acgtcaattc gttctggcag cgcgtcatca ccggcgc
//
LOCUS       DASYCS010000275         1620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_5144521,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000275 DASYCS010000000
VERSION     DASYCS010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5144521"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VGM46_08730"
     CDS             complement(<1..228)
                     /locus_tag="VGM46_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192719.1"
                     /translation="MTSTPAWIASPPEAQKTAQSRNAPARASLPGIAEGGACRFREGA
                     AIVWRRVFSYSGGAPQEGPMLRGLYDWTLSLA"
     gene            544..628
                     /locus_tag="VGM46_08735"
     tRNA            544..628
                     /locus_tag="VGM46_08735"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:578..580,aa:Leu,seq:caa)
     gene            complement(777..>1620)
                     /locus_tag="VGM46_08740"
     CDS             complement(777..>1620)
                     /locus_tag="VGM46_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006199910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HEY4192720.1"
                     /translation="KVEARNFDIRKNLLKYDDVSNDQRKVVFEQRLDLMDGEGLTEMV
                     TEMRTGVIDEIVAKNIPENAYAEQWDTATLKAEVEQFLNLDLPIEEWAKEEGIIEDEI
                     RERITAAADAAAADRAERFGPEVMVYVERSVVLQSLDALWREHIVNLDHLRSVVGFRG
                     YAQRDPLQEYKSEAFELFQALLGNLRQAVTAQLMRVELVRQAAEAPPPQAPEAFGSHI
                     DGTTGEDDFQGGETAILAKSEAVVAPENRDPNNPATWGKVGRNEACPCGSGKKYKHCH
                     GAFA"
BASE COUNT          269 a          518 c          511 g          322 t
ORIGIN      
        1 cgccaacgac aaggtccagt cataaagtcc gcgcagcatg ggtccctctt gaggtgcgcc
       61 gcctgaatag gaaaaaaccc ggcgccacac aatggccgcg ccttcacgaa atcgacaggc
      121 gccgccttcg gcgatgccgg gcaggctcgc gcgcgccgga gcgtttcggg attgggcggt
      181 tttttgcgcc tcgggcggtg aggcgatcca tgcgggggtg ctggtcaatg tgggtcatcg
      241 gccgagcaaa ccgacaaagc gggcggtcac tcgcccggaa gcccaaaggc cgggccgggc
      301 caaatccttc ggccttcgcc tcgaccgcgt caccaatacg atcctcgaca gggcctcgac
      361 cttgcccaga gccttaccgt tggaatgctg cccgctagtc agaagcgctc cggcgggcaa
      421 gactttcgca atcctgttga cgaccatggg tccgggcgtg tagttcgcgc ccatctactg
      481 tcggcgcctt gaagcccggc cactaggttc cgccgccgct tcggctggac ctgcaacgcc
      541 tgtgcgggtg tggtggaatt ggtagacgcg ctggactcaa aatccagttc cgaaaggagt
      601 gccggttcga ccccggccac ccgcaccatt tcccgacctc aggcctgttc gctgcgttga
      661 cccccttggg agacggcctt tgcgggccgg cggttctcat aaccggccgt taaaagcatt
      721 cggcgccgca tccgttggga tacagcgccg aattttttta gcgggatcgc ttcgcgtcag
      781 gcgaaagcgc cgtggcaatg cttgtatttc ttgccggaac cgcaggggca ggcttcgttg
      841 cggccgacct tgccccaggt cgccgggttg ttagggtcgc gattttccgg tgcgacgacg
      901 gcttcggact tggccagaat ggcggtttcg ccgccctgga aatcgtcttc gccggtcgtg
      961 ccgtcgatgt ggctgccaaa tgcttccggc gcctgcggag gcggggcttc ggcagcctgg
     1021 cgcaccagtt cgacgcgcat cagttgcgcc gtgacggcct ggcgcagatt gcccagaagc
     1081 gcctggaaca gctcgaacgc ctcgctcttg tattcctgga gcggatcgcg ctgcgcatag
     1141 ccgcggaagc cgacgaccga acgcaaatgg tcgaggttga cgatatgctc gcgccacagc
     1201 gcatccagcg actgcaacac caccgagcgc tcgacataga ccatcacctc cggcccgaag
     1261 cgttcggccc ggtcggcggc ggcggcatcg gcggcagccg tgatgcgctc gcggatctcg
     1321 tcctcgatga tgccctcttc cttggcccat tcctcgatcg gaagatcgag gttgaggaac
     1381 tgctcaacct cggccttgag cgtcgcggtg tcccactgct cggcataggc gttttcaggg
     1441 atgttcttgg cgacgatctc gtcgatgacg ccggtgcgca tctcggtgac catttcggta
     1501 aggccttcgc catccatcaa atcgaggcgc tgctcgaaca ccactttgcg ctggtcgttg
     1561 gagacgtcgt catatttcag caggttcttg cggatgtcga aattgcgcgc ctcgaccttg
//
LOCUS       DASYCS010000276         3816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3838396,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000276 DASYCS010000000
VERSION     DASYCS010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3838396"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="VGM46_08745"
     CDS             complement(<1..921)
                     /locus_tag="VGM46_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008598221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY4192721.1"
                     /translation="MASSETAPQPGLLTQFRQSLAGTDTEAVLRAGLIGIDATVDGPF
                     GPKKMVYADYVASGRALMQIEQFILEKVLPYYANSHTEASYCGGFITRLRREARQVVA
                     QSCGAGGQHGVIFTGAGATAGLNRLTALLGIVDAVAAGEKPCVIIGPYEHHSNVLPWR
                     ESGAEVIEIAEGPDGGPDLEMLRSVLAQTAGRKLVVGAFSAASNITGIVTDVVAVTRL
                     LNDASAKVVWDYAGGGPYLPIAMTPAPGVEIDAIVVSPHKFLGGPGSSGILILRHDAA
                     VTRKPTWPGGGTVRFVSPAGHDYSESLEARE"
     gene            1033..1503
                     /locus_tag="VGM46_08750"
     CDS             1033..1503
                     /locus_tag="VGM46_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008598220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY4192722.1"
                     /translation="MENLDSFDLKILLALQRDNSLSQRDLAEQVGLSQNAYWRRLQRL
                     TANGFVKGSRAVVDLSALGFDLTVFVMIKTRHHSKEWADGFRSHVGRLPEVVDFYRIG
                     GDWDYLLKVVTKGMAGYDGFYQKLITDFDLATVTGIFSMEAILENRPIGLNNPR"
     gene            1742..>3816
                     /locus_tag="VGM46_08755"
     CDS             1742..>3816
                     /locus_tag="VGM46_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019856651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY4192723.1"
                     /translation="MKTHAQVVVIGGGLMGCSILYHLAKHGWTDIVLLERSELTSGST
                     WHAAANIHGLHDSTNTSRLQHYTMALYKELETETGQGCGVFQPGSLYLAQTEAREHQL
                     RLQEAKARRYGMDFYEVSRTEAERLHPLVDFDGVRCIMFEPQGGNVDPSGVTMAYAAG
                     ARQRGAEIHRFTAVTGTEQQTDGSWIVRTDKGDIHTQWVVNAAGLWAREVAAMAGLKV
                     PLIPTEHQYFVTETIPEIAALDRRLPSVADRDGEYYLRQEGLGLLIGAYERNMKFWAE
                     DGTPLDFGHELFPDDLDRIEENMLRAIARVPAVGNAGVKKVINGPMIWSPDSSALFGP
                     VPELNNYFCCIGLIPGFSQSGGLGKLAAEWMIEGEPTLDMFAWDLARFGSWADKAFTK
                     ARVQDQYSHRFKIHFPNEERAAGRPVRTRAAYAMQKEMGGIFGLNYGWEHPLWFAAEG
                     EPREESIGFARQNWWEPVGREARMLRENVGIIDISNYAKYEVRGPGAEAWLNKLLANR
                     MPKEIGRSCLTPLIGKRGGIAGDFTVTRLGEEEFMVFGSGMAERFHQRYFKAVPLPAG
                     TTFTSMTQALCGFNVAGPKSRDLLQQLTNADLSTASFPFMRSRRIAVQGIDVIALRVS
                     FTGDLGWELHCAEADQAALYELLIAEARKDGGGPVGSRALMSLRVEKGYGSWSREYSP
                     EYWPQEVHM"
BASE COUNT          688 a         1202 c         1201 g          725 t
ORIGIN      
        1 ctcgcgcgct tcgaggcttt cgctataatc gtggccggcc ggcgagacga accgcaccgt
       61 gccgccgccc ggccatgtcg gcttcctcgt caccgccgcg tcgtggcgca ggatgaggat
      121 gccggaggaa cccggaccgc ccaggaattt gtgcggcgag acgacgatgg cgtcgatctc
      181 gacgccgggc gcgggcgtca tcgcaatggg caggtaaggt ccgccgccgg catagtccca
      241 gacgactttc gcactagcgt cgttcaacag gcgcgtgacc gccaccacat cggttacgat
      301 gccggtgatg ttcgaggccg ccgaaaacgc gccgaccacc agctttcggc cggccgtctg
      361 cgccagaacg ctccgcagca tttcaaggtc cggcccgcca tccggtccct ccgcgatctc
      421 gatgacttcc gcgccgctct cgcgccaagg cagcacattg gaatgatgtt catagggacc
      481 gatgatgacg cagggttttt cgccggccgc caccgcatcg acgatgccga gcagggcggt
      541 caggcggttc agtcccgccg ttgcgccggc gccggtaaag atcacgccgt gctgcccgcc
      601 cgccccgcag ctttgcgcga cgacctgccg ggcctcccgg cgcaggcgcg tgatgaagcc
      661 gccgcaatag gaagcttcgg tatggctgtt ggcataatag ggcagcacct tttcaaggat
      721 gaactgctcg atctgcatca gcgcgcgtcc cgaagcgacg taatcggcat agaccatctt
      781 cttcggcccg aagggaccgt cgaccgtagc gtcgatcccg atgaggccgg cgcgcagcac
      841 cgcttccgtg tctgtaccgg cgaggctctg ccggaactgg gtgagaaggc ccggctgtgg
      901 cgcggtttcg ctgctagcca tcgtgttttt gatccccatt tccggtcggc atcaacatta
      961 gaacgcaacc ggcattgatt cttcacctat tttcggacta tttgagcacg aaatgggtca
     1021 aagtcgcgaa aaatggaaaa tctggatagc tttgatctga aaatcctgct tgcgcttcag
     1081 cgcgacaaca gcctgtcgca acgcgatctg gccgagcagg tcggcctttc ccagaatgcc
     1141 tattggcgtc gcctgcaacg gctgaccgca aacgggttcg tcaagggttc gcgcgctgtg
     1201 gtcgatctgt cggcgctggg cttcgacctg acggtgttcg tgatgatcaa gacccgccac
     1261 cactcgaagg agtgggcgga tggcttccgc agccatgtcg gccgcctgcc ggaggtggtg
     1321 gatttctacc gtatcggtgg cgattgggac tatctgctga aagtcgtcac caagggcatg
     1381 gccggctatg acggcttcta ccagaagctg atcaccgatt tcgacctcgc cacggtgacc
     1441 ggcattttct cgatggaagc aatcctcgaa aaccgcccga ttggcttgaa caacccacgc
     1501 tgagacggcg atggcctggc gaaaaccggt ttgaagcgcg gaattccggc cttccggcag
     1561 cgcgccttcg ccgctctccg gtttgatggg ggctatgccc ccgttttttt tgagtgttgc
     1621 cggcgctaaa agatgctttt tgccgggcag gcgggttttt ccatcccgct gatatggttt
     1681 ggcctcgaaa aatcgaggtc ttcgctcatc cgcctaccac tcgacaaagg aagttccgtt
     1741 catgaagaca catgcgcagg tggtggttat cggcggtggc ctgatgggct gctcgatcct
     1801 ttatcacctc gccaagcatg gctggaccga tatcgtcctg cttgagcgct cggaactaac
     1861 ctccggctcg acctggcatg ccgccgccaa cattcatggc ctgcacgatt ccaccaacac
     1921 cagccgcctg caacactata cgatggcgct ctacaaggag ctggaaaccg agactggcca
     1981 gggctgcggc gtcttccagc ctggctcgct ctatctcgcc cagaccgagg cgcgcgagca
     2041 tcagctgagg cttcaggaag ccaaggcgcg ccgctacggc atggacttct acgaggtcag
     2101 ccgcaccgag gccgagcggc tgcacccgct ggtcgatttc gacggtgtcc gctgcatcat
     2161 gttcgagccg cagggcggca atgtcgatcc ttccggcgtg accatggcct atgcggcggg
     2221 cgcaaggcag cgcggcgccg aaatccatcg ctttacggcg gtgaccggca ccgagcagca
     2281 gaccgatgga agctggatcg tgcgtaccga caagggcgat atccacacgc aatgggtggt
     2341 caacgccgcc ggcctgtggg cgcgcgaagt cgccgccatg gccggtctga aggtgccgct
     2401 catcccgacc gagcaccagt atttcgttac cgaaacgatc cccgaaatcg ccgcgctcga
     2461 tcgccgcctg ccttcggtgg ccgaccgcga cggcgaatat tatctgcgcc aggaaggcct
     2521 tggccttttg atcggcgcct atgagcgcaa catgaaattc tgggcggaag acggtacgcc
     2581 actcgatttc ggccatgaac tgtttcccga cgatctcgac cgcatcgagg agaacatgct
     2641 gcgcgccatt gcgcgcgtgc cggctgtcgg aaacgccggc gtcaagaagg tcatcaatgg
     2701 cccgatgatc tggtcgccgg acagttccgc tctgttcggg ccggtgccgg agctgaacaa
     2761 ttatttctgc tgcatcggcc tcattccggg cttctcgcaa tcaggcggct tgggcaagct
     2821 ggccgccgaa tggatgatcg agggcgagcc gacgctcgac atgttcgcct gggatctggc
     2881 gcgcttcggc tcgtgggccg acaaggcctt caccaaggcc cgtgtgcaag accagtacag
     2941 ccaccgcttc aagatccatt tccccaacga ggaacgcgct gccggccgtc ccgtgcgcac
     3001 ccgcgccgct tatgcgatgc agaaggaaat gggcggcatc ttcggcctca attatggctg
     3061 ggagcatccg ctgtggttcg ccgccgaggg cgagccgcgc gaggaatcca tcggctttgc
     3121 gcgccagaac tggtgggagc cggtcggccg cgaagcgcgc atgctgcgcg aaaatgtcgg
     3181 catcatcgat atttcgaact atgcgaaata tgaggtgcgc gggccgggcg ccgaagcctg
     3241 gctgaacaag ctgctggcca accgcatgcc gaaggaaatc ggccgctcct gcctgacgcc
     3301 gctgattggc aagcgcggcg gcattgccgg cgatttcacg gtgacgcggc ttggcgagga
     3361 agagttcatg gtgttcggct ccggcatggc cgaacgcttc caccagcgct atttcaaggc
     3421 cgtgccgcta ccggccggca ccacgttcac ctcgatgacg caggcgctgt gcggcttcaa
     3481 tgtcgccggg ccgaagtcgc gcgacctgtt gcagcagctg accaatgccg acctttcgac
     3541 agcgtccttc ccgttcatgc gctcgcgccg gatcgcggtg cagggcatcg acgtgatcgc
     3601 gctgcgcgtg tcgttcaccg gggatctcgg ctgggaactg cactgcgccg aggccgatca
     3661 ggcagcgctt tatgaactgc tgattgccga agcccgcaag gatggcggcg gcccggtcgg
     3721 ctcgcgggcg ctgatgtcgc tgcgcgtcga gaagggctat ggcagctgga gccgcgaata
     3781 ctcgccggaa tattggccgc aggaggtcca catgga
//
LOCUS       DASYCS010000277         3973 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3694500,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000277 DASYCS010000000
VERSION     DASYCS010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3973
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3694500"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..870
                     /gene="otnI"
                     /locus_tag="VGM46_08760"
     CDS             73..870
                     /gene="otnI"
                     /locus_tag="VGM46_08760"
                     /EC_number="5.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HEY4192724.1"
                     /translation="MPRFSANLSMLFQEHDFLDRFDAAAAAGFKGVEYVGPYDHAPDV
                     VAARLKKNGLEQVLFNLPPGDWAKGERGIAVLPDRVEEFRASVDTAITYAKALGCPQV
                     NCLAGIAPAGAERQMLETVFVENLSFAADRLEKAGIKLLIEPINTRDMPGFFLNYSDQ
                     ALALINRIGSKNLFLQYDIYHMQIMEGDLARTMEENLGRIAHIQLADNPGRHEPGTGE
                     INYPFLYEHIDRIGYSGWIGAEYKPKAGTEAGLGWLRQLNGKGSVAA"
     gene            905..1792
                     /locus_tag="VGM46_08765"
     CDS             905..1792
                     /locus_tag="VGM46_08765"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008838065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0019580 - galactarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HEY4192725.1"
                     /translation="METIGFIGLGIMGAPMAGHLLDAGYPVVSSNHRSAPPADLVARG
                     LKTVAGHGAVAEAADIIILMVPDTPQVADVLFGENGVADGLAKGKLVIDMSSISPIET
                     KEFAKKINGLGCDYLDAPVSGGEVGAKAASLTIMVGGEEKAFERAKPVFDKMGKNVTL
                     VGPNGVGQTTKVANQIVVALTIEAVAEALVFASKAGADPAKVRQALMGGLAASRILEV
                     HGERMIKRTFDPGFRIELHQKDLNLALEGAKSLGISLPNTSTTQQLFNSCAANGGAKE
                     DHSALVRALERMANHEVAG"
     gene            complement(1842..2156)
                     /locus_tag="VGM46_08770"
     CDS             complement(1842..2156)
                     /locus_tag="VGM46_08770"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEY4192726.1"
                     /translation="MYVAMNRFQVMPGQEEAFEAVWKNRKSTLSEMKGFREFHLLRSE
                     FNAEKNSTLFASHTVWESKEDFVAWTKSENFRDAHKNAGTSKLTVFGHPQFEGFSSVE
                     GA"
     gene            complement(2276..>3973)
                     /locus_tag="VGM46_08775"
     CDS             complement(2276..>3973)
                     /locus_tag="VGM46_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006335376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY4192727.1"
                     /translation="NTYGSGAIGGVVFFDTKDATNFLKPDESWAGSMTGRYETNGQGW
                     TTSATGAYRFNENADVLGNIVYRNYDDYKDGDGDKVAGSGFDVLGGLLKTTIRPSENS
                     ELKLGWIGSSDNWNETSGGAPVSDLGLRQNTFTARYNITDEDKSWLDLHINASYNKTG
                     LDRTYLTTLSGGQFDPKTGLPTIIPAGAQTNYDLDTWSFDLWNTSRFETGALQHELTY
                     GGDWVSDDMVTQDPGGGMDTYTPSGKRRVSGGYVQDKVTWDWLEVIAGLRYDSYKLDS
                     ETADNSGDRLSPRITVGVSPFDTAALQGLQFYGTYAEGYRSPSITETLMNGMHPAGAT
                     FPFLPNPDLRPETGKTWEFGANYKTDGVIEAGDALRLKAAYFNNDVDDYIGSTTISAF
                     TDTTGLCPVRWDLLTNPDVTYAPICSQYQNFANAKIRGFEFEGVYDAAWGFAGLSASI
                     INGHTVDENGVRADLTTVPSSQLTGQLGFRFLEDRLTVGGEVQYNGKPKGNATAKDYT
                     LVNAFASYQANENYKFDFRVDNIFDVKYANPLNATTTSTLYEPGVTLKLAATMRFGG"
BASE COUNT          746 a         1140 c         1289 g          798 t
ORIGIN      
        1 ttcgaggacc ttgccgaacg ccacgaagac gcaccgactg cgatcgtcat gcttgactga
       61 aggaatatag aaatgccgcg cttttcagca aatctctcca tgctttttca ggagcatgat
      121 tttctcgacc gcttcgacgc cgctgccgcc gccggcttca agggcgtcga atatgtcggc
      181 ccctacgacc acgcgcccga tgtcgtcgcg gcgcggctga agaagaacgg tctggagcaa
      241 gtgctgttca acctgccgcc cggcgactgg gcgaagggcg agcgcggcat tgcggtactg
      301 cctgaccgcg tcgaggaatt tcgcgcaagc gtcgacaccg ccatcaccta tgcgaaggcg
      361 cttggctgcc cgcaggtcaa ttgcctggcg ggtatcgcgc cggcgggcgc cgagcggcag
      421 atgctggaga ctgtcttcgt cgaaaacctg agttttgcgg ccgacaggct ggaaaaggcg
      481 gggatcaagc tgctgatcga gccgatcaac acgcgcgata tgccgggctt cttcctcaat
      541 tattccgacc aggcgctggc gctcatcaac cgcatcggtt cgaagaacct gttcctgcaa
      601 tatgacatct atcacatgca aatcatggag ggggatctcg cccgtaccat ggaggaaaac
      661 ctcggccgca tcgcgcatat ccagcttgcg gacaatcccg gccgtcatga gccggggacg
      721 ggcgagatca attatccgtt cctctacgag cacatcgacc gcatcggtta ttccggctgg
      781 atcggcgccg aatacaagcc gaaggcgggt acggaggcgg ggcttggctg gcttcgccaa
      841 ttgaacggca aaggcagcgt cgcagcctga gcacaagtgc gtaactatct ggaaggacag
      901 ggaaatggaa accatcggct tcatcggcct tggcattatg ggcgcgccta tggcggggca
      961 tctgcttgac gccggttacc ctgtcgtgtc gtcgaaccac cgctccgcgc cgccggccga
     1021 tctggttgcc aggggcctga agacggtggc cggccacggc gccgtcgccg aggccgccga
     1081 catcatcatc ctgatggtgc cggatacgcc gcaggtggcc gacgtgctgt ttggcgagaa
     1141 cggcgtcgcg gatgggctgg ccaagggcaa gctcgtcatc gacatgagtt ccatttcgcc
     1201 gatcgaaacc aaggaattcg ccaaaaagat caacggactt ggctgcgact atctcgatgc
     1261 gccggtgtct ggcggcgagg ttggcgcaaa ggccgcgtcg ctgaccatca tggtgggcgg
     1321 cgaggaaaag gcgttcgaac gggcaaagcc ggtcttcgac aaaatgggca agaacgtcac
     1381 gctggtcgga ccgaacggcg tcgggcagac caccaaggtg gcaaaccaga tcgtcgtggc
     1441 gctgaccatc gaggcggtgg ccgaagcgct ggtattcgca tcgaaagccg gcgccgatcc
     1501 ggctaaggtc aggcaggcct tgatgggcgg gctggcggcc tcgcgcattc tggaggtgca
     1561 tggcgagcgc atgataaagc gcaccttcga ccccggtttt cgcatcgaat tgcaccagaa
     1621 agatctcaat ctggcgctcg aaggggcgaa atcgctcggc atttcgctgc cgaacacctc
     1681 gaccacgcag cagcttttca attcgtgcgc ggcaaacggc ggcgcgaagg aagaccactc
     1741 ggcgctggtg cgggcgctgg agcggatggc taatcacgag gtggccggct gaaattccgt
     1801 gacagaaagg cccggcgcgg cgacgcggcg ggcctttttg gtcatgcgcc ctcgacggaa
     1861 gaaaagccct cgaactgcgg atggccgaaa acggtcagct tcgaggtgcc ggcgttcttg
     1921 tgcgcgtcgc gaaagttttc ggacttcgtc caggcgacga agtcttcctt gctttcccaa
     1981 acggtgtgcg aggcaaacag ggtggagttc ttctccgcgt tgaattcgct gcgcaacagg
     2041 tggaattccc ggaacccctt catttccgaa agggtggatt tgcggttttt ccagacagcc
     2101 tcaaacgcct cctcctggcc cggcatcacc tggaaacggt tcatcgcgac gtacatggca
     2161 gtcctttctg gtcggtgtaa gctcgtctaa tcggggcggc gggagtgaag ccattgccgt
     2221 ccgatggtgt agagcggcgg aagcggtgaa ccgctgccgc cgttctggaa cgttcctagc
     2281 cgccaaagcg catcgttgcc gccagcttca gcgtcacgcc cggctcgtag agggtgctcg
     2341 tggtcgtggc gttgagcgga ttggcgtatt tgacatcgaa gatattgtcg acgcggaaat
     2401 cgaacttgta gttttcattt gcctggtagc tggcgaaagc attgaccagc gtataatcct
     2461 tggccgtcgc attgcccttg ggcttgccgt tgtactgaac ttcgccgccc accgtcagcc
     2521 tgtcttcaag gaagcggaag ccgagctggc cggtaagctg ggaggacggc accgttgtca
     2581 gatcggcccg cacgccgttt tcgtccacgg tgtggccgtt gatgatggaa gcggagaggc
     2641 cggcgaagcc ccaggcggca tcgtagacgc cttcgaattc gaaacctctg atcttcgcat
     2701 tggcaaagtt ctgatactga gagcagatcg gcgcataagt cacatcgggg ttggtcaaca
     2761 aatcccagcg caccgggcac aggccggttg tatcggtaaa cgccgaaatc gtcgtgctgc
     2821 cgatgtagtc gtcgacatcg ttgttgaagt atgcggcctt caggcgaagc gcatcaccag
     2881 cctcgatgac gccgtcggtc ttgtagttgg cgccgaattc ccaggtcttg ccggtttcag
     2941 gccgcaggtc cggattgggc aggaagggga aggtggcacc ggcaggatgc atgccgttca
     3001 tcagcgtttc ggtgatggag ggcgagcgat agccctcggc ataggtgccg tagaactgga
     3061 ggccttgcag cgctgccgtg tcgaatggcg agacgccgac ggttatgcgc ggcgacaggc
     3121 ggtcgccgga attgtcggcg gtttcgctat cgagcttgta gctgtcatag cgcaggccgg
     3181 cgatcacttc gagccaatcc caagtaacct tgtcctggac gtagccgccg cttacccgcc
     3241 gcttgccgga aggcgtgtag gtgtccatgc cgccgcccgg atcctgcgtg accatgtcgt
     3301 cgctcaccca gtcgccgcca taggtcagtt catgctggag cgcgccggtt tcgaagcgcg
     3361 acgtgttcca gaggtcgaag ctccaggtat cgaggtcgta gttggtctgc gcgccggccg
     3421 ggatgatggt tggaaggccg gtcttcgggt cgaactggcc accgctgagc gtcgtcaggt
     3481 aggtccggtc gaggcccgtc ttgttgtagg aagcgttgat gtgcaggtcc agccagctct
     3541 tgtcctcgtc ggtgatgttg tagcgtgcgg taaacgtgtt ctgccgcagg ccaaggtccg
     3601 aaaccggagc gccgccgctg gtttcgttcc agttgtcgct ggagccgatc cagccgagct
     3661 tcagctcgct gttctcgctg gggcggatag tggtcttgag caggccgcca agcacgtcga
     3721 atccggagcc ggcaaccttg tcgccatcgc catccttgta gtcatcatag ttgcgataga
     3781 cgatattgcc gagcacatcg gcgttttcat tgaagcggta agcgcccgtg gcgctggtgg
     3841 tccagccctg gccgttcgtt tcgtagcggc cggtcattga gccggcccag ctttcgtcag
     3901 gtttcaggaa attggtcgcg tccttggtgt cgaagaagac gacgccgccg atcgcgccgg
     3961 aaccgtaggt gtt
//
LOCUS       DASYCS010000278         2557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4127720,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000278 DASYCS010000000
VERSION     DASYCS010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4127720"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..1006)
                     /gene="coxB"
                     /locus_tag="VGM46_08780"
     CDS             complement(140..1006)
                     /gene="coxB"
                     /locus_tag="VGM46_08780"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008839344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEY4192728.1"
                     /translation="MKRCLAGAAAVATLFTTVVAHAAQPAAWEMNFQPAATDIMRQIT
                     WFEHYTLWFVVPITLFVLVLLAYCIVKFRASVHPVASRTSHNTLIEVIWTVGPVLVLL
                     CIAIPSFQLLTAQYTPPEEAKLTVKATGNQWNWDYEYQVDQPLSFNSALLDDGDRAGL
                     GKSDKAVYPRLLAVDHEMVVPVDTMTRVLVTATDVIHAFAMPSFGIKTDAVPGRTNEV
                     WFKAEKEGLYYGQCSELCGQNHAFMPIAIRVVSEAQYNTWLAAAKSDLAGANKALMAE
                     VDSSNKVAAAGN"
     gene            1377..1892
                     /locus_tag="VGM46_08785"
     CDS             1377..1892
                     /locus_tag="VGM46_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006330608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HEY4192729.1"
                     /translation="MNAWLSRTLAKVIFLAAISCAGNAFAAQPSGTVKSTHGAWSIIC
                     DTPAGATAEQCVMMQNVVAEDRPEMGLSVVVLRTADNKAEILRVLAPLGVLLPNGLGL
                     NVDGKDIGRAYFVRCFQDGCYAEVILEKPLLDTLKTGKAATFIVFQTPEEGIGIPVDL
                     KGFGEGFAALP"
     gene            1965..>2557
                     /locus_tag="VGM46_08790"
     CDS             1965..>2557
                     /locus_tag="VGM46_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HEY4192730.1"
                     /translation="MNSLLGQFDVSDDRIKQIVADTIKGADDGELFLEYGESEALMFD
                     NGKLKTANFSTDQGFGLRAVVGEASGYAHSSDLSEAALLRASDAVSAVKGGYSGTLAA
                     APAGTNRHLYGDENPIPSPSFETKAKLLQEIDAWLRAQDPRVRQVTASLASSWQQVEI
                     LRADGQVARDVRPLVRVNVSVVVGDGDRQESGSYGMGG"
BASE COUNT          486 a          776 c          797 g          498 t
ORIGIN      
        1 tagagcgtac cgatatcctt gtggttggtc gaatagaccc aacgtaccca acccttatag
       61 ggcgtgcgtc catgatcgtc gtgagcagca gcatgtgcca tgggtggctc cgttctctga
      121 tctctgcttg gccgcggcat caattgccgg cggccgctac cttgttcgaa ctgtcgactt
      181 cggccatcag cgccttgttg gccccggcaa ggtcgctctt ggcggccgcc agccacgtat
      241 tgtactgcgc ctcggagacg acgcggatgg cgatcggcat gaaagcgtgg ttctggccgc
      301 acagttccga gcactggcca taatacaggc cttccttttc cgccttgaac cagacctcgt
      361 tggtccggcc ggggacggcg tccgtcttga tgccgaagga cggcatggcg aaggcatgga
      421 tgacgtcggt ggcggtgacc agcacgcggg tcatggtgtc gaccgggacg accatctcat
      481 gatcgacggc caaaaggcgc gggtagacgg ccttgtccga cttgccgagg ccggcgcggt
      541 cgccgtcgtc gagcagcgcg gagttgaacg agagcggctg gtcgacctgg tattcataat
      601 cccagttcca ctggttgccg gtcgccttga cggtcagctt ggcttcttcc ggcggcgtat
      661 attgcgccgt cagcagttgg aacgaaggga tggcgatgca cagaagcacc aataccggcc
      721 caaccgtcca gatcacttcg ataagcgtgt tgtggctggt gcgcgaggcc accggatgga
      781 cgctcgcacg aaacttcacg atacaataag cgagcaaaac cagcacgaaa agggtgatcg
      841 gcacgacaaa ccacagcgta taatgctcaa accaggtgat ctggcgcatg atatcggtgg
      901 ccgccggctg gaaattcatt tcccatgcag cgggctgggc ggcgtgggcg acgaccgtcg
      961 taaacaaggt ggcgaccgcg gctgcacccg caaggcatct cttcatcgct ctcatcattt
     1021 cccccgcgat aggccgcaag caattggaac aacgccggaa cgagaatccc gggcactttg
     1081 gtatccggtt caaaccatac tccttttcgc tccgcaagaa aggagcaccg ctacccatgc
     1141 ggcatttttg cgcgcgcaag ctggcattcg ccgtcggacc caccttgcgc cttgccgttc
     1201 ggccggtccg atggatttgg cgaaatcgcg cgagccggat ggttttgcct taaatcgggt
     1261 gaaatcgccg cggaaaaacg tattatcgcc gcagtcgcgg cagattgagc gcggccgcat
     1321 gcttttactt ggctttggcg cagcttacag tgccgtgatt cgcaaatgga gccgtcatga
     1381 acgcttggct ttcgagaacc ttggcgaagg tcatctttct cgccgccata tcgtgcgctg
     1441 gaaacgcctt tgccgcacaa ccgagcggca cggtgaaatc gacgcatggc gcgtggtcga
     1501 tcatctgcga cacgcctgcc ggcgcaaccg ccgaacaatg cgtgatgatg cagaacgtcg
     1561 tcgccgagga ccgccccgaa atggggcttt ccgtggtcgt gctgcgcacc gccgacaaca
     1621 aggcggaaat cctgcgcgtg ctggcgccgc ttggcgtatt gttgcccaat ggccttggcc
     1681 tgaacgtcga cggcaaggat attggccgcg cctatttcgt gcgctgcttc caggacggct
     1741 gctatgccga ggtcattctg gaaaagccgt tgctcgatac gctgaagacc ggcaaggccg
     1801 ccaccttcat cgtgtttcag acgccggaag aaggcatcgg catcccggtt gacctcaagg
     1861 gcttcggcga aggtttcgct gcacttccct gaccggcccg cattgccgaa acgcctcggc
     1921 gcgcctacat cgggggaaag acattccctg aggatttccg tgccatgaac agcctgctcg
     1981 gccaattcga cgtttccgac gatcgcatca agcaaatcgt cgccgacacc ataaagggtg
     2041 ccgacgatgg cgagttgttc ctcgaatatg gcgagagcga agcgctgatg ttcgacaacg
     2101 gcaagctcaa gaccgccaat ttcagcactg atcagggttt cgggctgcgc gcggtggtcg
     2161 gcgaggccag cggctatgcc cattccagcg acctttccga agcggcgctg ctgcgcgctt
     2221 ccgatgcggt atcggcggtg aagggcggtt attcgggaac gctggcggca gcgcccgccg
     2281 gcaccaaccg gcatctctat ggcgacgaaa acccgatccc ctcgccctcc ttcgagacca
     2341 aggcgaagct tttgcaggaa atcgacgcct ggctgcgcgc gcaggacccg cgcgttcggc
     2401 aggtgacggc ctcgctcgcc tcgtcctggc agcaggtgga aatcctgcgc gccgacggac
     2461 aggtggcgcg cgatgtgcgc ccgctggttc gcgtcaacgt ctccgtggtg gtcggcgacg
     2521 gcgaccggca ggaatcgggt tcctatggca tgggcgg
//
LOCUS       DASYCS010000279         9681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2534529,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000279 DASYCS010000000
VERSION     DASYCS010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 9681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9681
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2534529"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /gene="fliI"
                     /locus_tag="VGM46_08795"
     CDS             complement(<1..327)
                     /gene="fliI"
                     /locus_tag="VGM46_08795"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860946.1"
                     /note="involved in type III protein export during
                     flagellum assembly; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HEY4192731.1"
                     /translation="MTSDQSPMRVDERSADSEPTGPLPALERLWQRFQNDDLLLRQGG
                     RITEVSATHYNARGLSGIARLGDLVEHRSHAGARSGEIVKIAREEVVVAPYERSADAG
                     IGDAVFR"
     gene            complement(337..1059)
                     /gene="flgF"
                     /locus_tag="VGM46_08800"
     CDS             complement(337..1059)
                     /gene="flgF"
                     /locus_tag="VGM46_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HEY4192732.1"
                     /translation="MQDSLYVSLSAQIALERRLDTIADNVANASTVGFRATGVKFEDV
                     VSGSGPKSVSFTSSGNTYLSSAHGALTETGNPFDFAVQGDAWFGIETPAGTVMTRDGR
                     FSMNENGELMSIEGHPVLDAGGSPLQLDPRGGPPTAGSDGSLRQGGQLVGTIGLFDFD
                     PGKNFVRYGNSGIVPAREPEPMTDRADIGIAQGFVEESNVNPVLEMTRLISVQRAFEN
                     TAAIMRQTTASSDDAIKTLGSK"
     gene            complement(1067..2878)
                     /locus_tag="VGM46_08805"
     CDS             complement(1067..2878)
                     /locus_tag="VGM46_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1217 domain-containing protein"
                     /protein_id="HEY4192733.1"
                     /translation="MLNTYTSYQLIARDIPKAIGQVESQPVVKRETDYYLANIGKVKS
                     IDDFVNNDRLFKYAMKAFGLEDMDYAKAFMVKALKEGVTDPDSFANKLTDKRYADFVS
                     AFNFAKLGENATTYNPAQQGVTQNYVLQVGLGGVQAGFNDYRTETANYLTNISSVKSI
                     DDLMGNDRLLTYAMAAFGLDAQTEPPERVRAMLEGGVADPNSPANTNPDKKYAAFVAA
                     FDFNQYGDQTTGKDAVQQAVPKGYMAGTGLVLPKVSAAYIKGEADYYAKNISKVKSVD
                     DLMKDQRLLTFAMAAYGLDASTETPKTIRAMLEGGVTDPASPANALADKRYATFVAAF
                     DFAQYGDQATTRDDVQKLTPKLYTSQSQLGLIKPNKDYVLAETDYYSANIGNVKSVDD
                     LMADDRLYNYTLAAYGLDPAKEDKNLIRSVLEGGIRDPGSVANKLTNKAYAGMAAALN
                     FEQYGESATTYNPTQQATVDKYARQTLEEDAGKTNEGVRLALNFARKAPDITNWYDVL
                     ADTALASVVRTSLGLPDSFATADIDKQAALFESRLDITDFTDPVKLNKFLTRFTSLYE
                     VNHPTSSAVTSVSVLFAQPTTIGISTDLMIAMQNLRY"
     gene            3017..3895
                     /gene="motA"
                     /locus_tag="VGM46_08810"
     CDS             3017..3895
                     /gene="motA"
                     /locus_tag="VGM46_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018011388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HEY4192734.1"
                     /translation="MSILIGLVVTLGCVLGGFMAMGGHLQVLIQPYEAIVIGGAALGT
                     FIVANPMKTIKDTGKGIMEALKGAVPKEPDYLETLGVLHSLMRELRSKSRSEVEAHID
                     NAEESAIFQAFPTVLKNHDLRNFICDYCRIIIIGNARSHEIEALMDEEIQTIRTDKMK
                     AYHAMIAVGDGLPALGIVAAVLGVVKAMGALDQSPEVLGELIGAALVGTFLGIFLSYA
                     VVGPIASKIKTVREKKNRLYVIVKQTLLAYMNGSLPQVAIEFGRKTISSHDRPSIDAV
                     EQSTMNAGGGADKKAA"
     gene            3931..4866
                     /locus_tag="VGM46_08815"
     CDS             3931..4866
                     /locus_tag="VGM46_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HEY4192735.1"
                     /translation="MTDAAATSAENRALIVERLVGETGEPNQVMAAARALAERALPAI
                     MLGLQQNMATSISMEIAAIELVRFIEARPPATNHAMTIAASASSPDALVLSIDNDALA
                     VVVSTLFGGDPQDAVAPIARELSPLETEVATMIFEQVAQAINGSGPRAFEFKLPLPLA
                     ITGTEMMKHILRDGPGVRVVFSMATPMSRGTMALMMPQRVLLKHRGDTGAPAKDAAPA
                     TEWRDRFSEEVMRSTVTLEATMPLGRLTLGDLDGLDVGQVIELEETAQTQARLSARGQ
                     TLFICEFGKLGQNYTVRIRHPFDAGQDFIDALLPG"
     gene            4895..5281
                     /gene="fliN"
                     /locus_tag="VGM46_08820"
     CDS             4895..5281
                     /gene="fliN"
                     /locus_tag="VGM46_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HEY4192736.1"
                     /translation="MSQTEPEAGIDMPDEQLDRAIEELRGVLKEEEKRPDAAFRAAGS
                     TNSGIIMNIPVEVQIVLGSTEMPVSDLMSLQKGSTVALNRRIGEPVDVVVNGRRIARG
                     EITVLESDPSRFGIRLTEIVAAAKSA"
     gene            5322..6335
                     /locus_tag="VGM46_08825"
     CDS             5322..6335
                     /locus_tag="VGM46_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007008641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HEY4192737.1"
                     /translation="MTQLVLTRAQKAAAILVAMGKPSASRLLKFFKQEELKALIEGAR
                     LLRTIPQSDLERIVTEFEAEFTEGAGLLDSADKMDTILNESLSPEEVSAIMAGKQPEA
                     AQPGPPPIWPDLEKLEPARLGAFLAGEHAQTAAMVLSKLSPQAAASVLLTLDKPLRNT
                     VIGRMVTLSAVPETATKIVENQLRMRVMGETSAKDNSAGQVRVASLLNEMDKSQLDEV
                     MQDLEQSGTPDLAAIRARLFSFEDILLLTQKARVALFDGLSSEQVTLSLRGAPADMVE
                     AVLSSIGARSRRMIESELGQAADGVAPADIVQARKTIATTAIRMAREGAFELPSTQTA
                     EAA"
     gene            6352..7434
                     /gene="flhB"
                     /locus_tag="VGM46_08830"
     CDS             6352..7434
                     /gene="flhB"
                     /locus_tag="VGM46_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HEY4192738.1"
                     /translation="MADAVDKDSKTEEATEKKIRDTIEQGKLPHSRETALLASFVAIL
                     IYTVFYAKDAFVDLGSFLSMFLEKPEAWPMDSETDVIELYKTVMMATTRAVTSLLVLL
                     VIAGVGSSVFQNIPQFVAERITPQLSRISIAKGWSRLFGVQGFVEFLKSLAKVGIACT
                     VLVFTLSQDHRRLLAGMMTNPLEFPMVIRGITVDILVTIVFVMALIAGVDLVWSRFHW
                     KQEMMMSKQEVKDEFKQSEGDPIIKSRLRSLARDRARKRMMTAVPRATLIIANPTHFS
                     IALKYVREEDSAPLVLAKGQDLVALKIREIAKEHNIPIFEDVALARSMYKQVSVDSVI
                     PSQFYQAVAELVRVVYSRKAGRKQVS"
     gene            7454..7891
                     /locus_tag="VGM46_08835"
     CDS             7454..7891
                     /locus_tag="VGM46_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192739.1"
                     /translation="MNRQPHASTREIIVAGAIEQVVQELRLVDVADYIAFIRLEHFAC
                     LSDLVDSAAELFFLPGTLRLGHGGEAHVDWGSSPRIELDLELRPKGATVYFRLTLSEH
                     GASVVVNYVSFEEPDEDPSRNTALLEAAIEQARMRRSEPLAFR"
     gene            complement(7896..8681)
                     /locus_tag="VGM46_08840"
     CDS             complement(7896..8681)
                     /locus_tag="VGM46_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY4192740.1"
                     /translation="MKNAFAAQAEIREAAEALFNEHRNPFGAFTLGSETHQIITIPDA
                     VRRCRWIAVDLHASGFGLFFVSPSPERARLVPCFDNEYPSIAVATKFMSGANGEDMVR
                     HTGVSTEPRWWADDGVPGSDAAFRNLAWTEAMTPLVPGTNGIAFPVHAERGQCGLAVF
                     LGSEIAPSQDLLYEIHARCFSLFSAVARTRPGDSGRTRAISKRELECLKLTANGYTSE
                     AIARLLKLSVHTANQYLSQCAQKLNAVSRTQAVAKALRLGLIE"
     gene            complement(8678..9397)
                     /locus_tag="VGM46_08845"
     CDS             complement(8678..9397)
                     /locus_tag="VGM46_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEY4192741.1"
                     /translation="MNGPVALLDKDPSDGPRTHISCRGDLTGFFMNLCAEIGADSYML
                     VAIVHDQDKNDARIVSSNWIFDAIELIGKRLIAGLAQGMLTAAPGSRPRPLTASEAPD
                     AVGLVSGEEARLLDVLGHSEIYSLRLNVGRQRLSVLFSSAKAGGIDTTELMKAQLKCC
                     YALSQIPQLLAQAAMQDPLSDRERECLFWVSEGKTTDEVAVILGVSSNTVNSYITHAI
                     QKFSASNRAMAIATAIRSGII"
BASE COUNT         1818 a         2879 c         3132 g         1852 t
ORIGIN      
        1 tcggaacacg gcatcgccga tgccggcgtc ggcgcttctt tcataaggcg caacgacaac
       61 ctcttcgcgg gctatcttga cgatctcgcc gctgcgcgcg ccggcatggc tgcgatgctc
      121 gacgagatcg ccaagccggg caatgccgga aaggccgcgc gcattgtaat gggttgcgga
      181 cacttcggtg atgcggccgc cttggcgcag caaaagatcg tcgttttgga agcgctgcca
      241 caggcgctca agcgctggca acgggcctgt cggctcgcta tcggcgctcc gctcgtccac
      301 acgcatcggg gattggtcgg atgtcattca gcgccgctac ttggaaccaa gcgtcttgat
      361 ggcgtcgtcg ctggaggcgg tggtctggcg cattatggcg gccgtgttct cgaaggcgcg
      421 ctggacgctg atcaagcggg tcatttccag aaccggattg acgttggatt cctcgacgaa
      481 cccctgcgcg atgccgatgt cggcccgatc ggtcatcggt tccggttcgc gcgccggcac
      541 gatgccggaa ttgccgtaac ggacgaaatt cttgcccgga tcgaaatcga acaggccgat
      601 ggtgccgacg agctggccgc cctggcgcag cgaaccgtcc gaaccggcgg tgggcgggcc
      661 gccgcgcgga tcgagctgca acggcgagcc gccggcgtcg agcaccggat ggccttcgat
      721 ggacatcagc tcgccgttct cgttcatgga gaagcggccg tcgcgggtca tcaccgttcc
      781 cgccggggtt tcgatgccga accaggcatc gccctggacc gcgaagtcga acggattgcc
      841 ggtttcggtc agcgcgccat gcgccgaaga cagataggtg ttgcccgacg acgtgaagga
      901 aaccgatttc ggcccgctgc ccgagacgac atcctcgaat ttcacgccgg tggcgcgaaa
      961 cccgaccgtc gaggcattgg cgacgttatc ggcgatcgtg tccagccggc gttcaagcgc
     1021 gatctgtgcc gagagggaga cgtaaagact gtcctgcatc gaatcctcag tatctcaaat
     1081 tctgcatggc gatcatcagg tcggttgaga tgccaatggt ggtcggctgc gcaaacagca
     1141 cgctgacgga agtcaccgcg ctggaagtcg ggtggttgac ttcgtaaagg ctggtgaagc
     1201 gggtgaggaa cttgttgagt ttcaccggat cggtgaaatc ggtgatgtcg agcctgctct
     1261 cgaacagcgc cgcctgcttg tcgatatcgg cggtggcgaa agaatcgggc aggccaaggg
     1321 aagtgcgcac cacggaagcc agcgccgtat cggccagtac atcgtaccag ttggtgatgt
     1381 cgggtgcctt gcgggcaaag ttgagcgcca agcgaacgcc ttcattggtc ttgccggcat
     1441 cttcctccag cgtctggcgc gcatatttgt cgacagtcgc ctgctgcgtc ggattatagg
     1501 tggtggcgct ctctccgtat tgttcgaagt tgagcgcggc cgccatcccg gcataggctt
     1561 tgttggtcag cttgttggcg acgctgccgg ggtcgcggat accgccttcg aggacgctgc
     1621 ggatgaggtt cttatcctcc ttcgccgggt ccagcccata ggcggcgagg gtgtaattat
     1681 aaagccggtc gtcggccatc agatcgtcga cggacttcac attgccgata ttggccgaat
     1741 agtagtccgt ctcggccagc acatagtcct tgttcggttt gatgaggccg agctgcgatt
     1801 gggacgtgta gagcttcggc gtcagtttct gcacatcgtc gcgcgtcgtc gcctggtcgc
     1861 catactgggc gaagtcgaag gcggcgacga atgtggcata gcgcttgtca gccaaggcgt
     1921 ttgccgggct ggcggggtca gtgacgccgc cctccagcat cgcgcggatg gtctttggcg
     1981 tctcggtgga tgcatccagc ccgtaggccg ccatggcgaa ggtcagcagg cgctggtcct
     2041 tcatcaggtc gtcgaccgat ttcaccttgg aaatgttctt ggcgtagtag tcggcctcgc
     2101 ccttgatgta ggctgccgat actttgggga gcaccagtcc ggtgccggcc atgtagcctt
     2161 tcggcacggc ctgctgcacg gcgtccttgc cggtcgtctg gtcgccatac tggttgaaat
     2221 cgaaagcagc gacaaaggcc gcatatttct tgtcaggatt ggtgttggcg gggctgttcg
     2281 ggtcggcgac gccgccttca agcatggcgc gcacgcgctc gggcggctcg gtttgcgcgt
     2341 ctaggccgaa tgcagccatg gcgtatgtga ggaggcgatc gttgcccatc aggtcgtcga
     2401 tcgacttcac cgaggaaatg ttggtcaggt aattggccgt ctcggtgcgg tagtcgttga
     2461 aaccggcctg aacgccgccg aggccgacct gaagcacata gttctgcgtg acgccctgct
     2521 gcgccggatt gtaggtggtg gcgttttcgc cgagcttggc gaaattgaag gcggagacga
     2581 aatcggcgta gcgcttgtcg gtcagcttgt tggcgaagct gtccggatcg gtgacgcctt
     2641 ccttcagcgc cttgaccatg aaagccttgg catagtccat gtcttcaagt ccgaacgcct
     2701 tcatcgcgta tttgaaaagg cggtcgttgt tgacgaagtc gtctatgctt ttgaccttgc
     2761 cgatattggc cagataatag tcggtttcgc gcttcaccac aggctgcgac tcgacctggc
     2821 cgatcgcctt gggaatgtcg cgggctatca gctgatagct cgtataggtg ttcaacatgg
     2881 cgcgtctccg gccgactcca ccaccaagat aaccggactt ccttgcgcga agctgaatct
     2941 gggcgtggtc atgattcaag cctcacacaa ggcacggggg ctatcgatgc tgccggtagg
     3001 actggaagga acgcgcgtga gcattctgat cggattggtg gtgacgctcg gctgcgtgct
     3061 tggcggcttc atggccatgg gcgggcatct gcaagtgctg atccagccct atgaggccat
     3121 cgtgatcggc ggcgccgcgc tcggcacctt catcgtggcc aacccgatga agacgatcaa
     3181 ggataccggc aaaggcatca tggaagcgct gaaaggggcg gtgccgaagg aaccggacta
     3241 cctggaaacg cttggcgtgc tgcattcgtt gatgcgcgaa ctgcgctcca aatcccgcag
     3301 cgaggtcgag gcgcatatcg acaatgccga ggaatcggcg atcttccagg cattcccgac
     3361 cgtgctgaaa aaccacgacc tgcgtaactt tatttgcgat tattgccgca tcatcatcat
     3421 cggcaatgcg cgttcgcacg aaatcgaagc gttgatggac gaggaaatcc agacgatccg
     3481 caccgacaag atgaaagcct accacgccat gatagcggtg ggcgacggtt tgccggcgct
     3541 cggcatcgtc gcagccgtgc tgggcgtcgt caaggcgatg ggcgcgctcg accagtcgcc
     3601 ggaggtgctg ggcgaactta tcggggccgc ccttgtcggc acgttcctcg gcatcttcct
     3661 gtcctatgcg gtggtcggcc ccatcgcctc caagatcaag acggtgcgcg aaaagaagaa
     3721 ccgcctctac gtgatcgtca agcagacgct gcttgcctac atgaacggtt cgctgccgca
     3781 ggtggcgatc gaattcggcc gcaagacgat ctcgtcgcac gaccgtccgt cgatcgacgc
     3841 ggtcgagcag agcacgatga acgccggtgg cggcgccgac aagaaagcgg cctgagccat
     3901 gtccggcacg gttactggga accggcaagc atgaccgacg cggccgccac ctccgctgaa
     3961 aaccgcgcgc tcatcgtcga gcgcctggtc ggtgagaccg gcgagccgaa ccaggtgatg
     4021 gcggcggcgc gtgcgttggc cgagcgagcc ttgccggcta tcatgcttgg cctgcaacag
     4081 aacatggcga cttccatatc catggaaatc gcggctatcg agcttgtccg cttcatcgag
     4141 gcgcggccgc cggctacgaa ccacgcgatg accatagcgg catctgcgtc ctcgccggac
     4201 gcgctggtgc tgtcgatcga caatgatgcg ctggcggtcg tcgtcagcac gctgttcggc
     4261 ggcgatccgc aggatgcggt cgcgccgatc gcgcgggaac tgtcgccgct cgaaaccgaa
     4321 gtggcgacga tgatattcga gcaggtggcc caggccatca acggttcggg tccgcgggcc
     4381 tttgaattca agctgccgct gccgttggcc atcaccggca cggagatgat gaagcacata
     4441 ttgcgcgacg ggccgggcgt gcgcgtggtt ttttcgatgg cgacgccgat gtcgcgcgga
     4501 acgatggcgc tgatgatgcc gcagcgggtt ttgctgaaac atcgcggcga caccggcgcc
     4561 ccggccaagg atgccgcgcc ggcgacggaa tggcgcgacc gcttttccga agaagtcatg
     4621 cgctcgaccg tgacgctgga agcgacgatg ccgctggggc ggctgacgct tggcgatctt
     4681 gacggtctcg atgtcgggca ggtcatcgag ctggaagaga cggcgcagac ccaggcgcgg
     4741 ctttcggcgc gcgggcagac gctgttcata tgcgaattcg gtaaactcgg gcagaattac
     4801 accgtgcgaa tcaggcaccc gttcgacgcc gggcaggatt tcatcgacgc actgctgcct
     4861 ggctgaggcc gggccgcacc tttggagacg acgcatgagc cagactgaac cggaagccgg
     4921 gatcgacatg cccgacgagc agctcgaccg ggccatcgag gaactgcgcg gtgttttgaa
     4981 ggaagaggaa aagcgccccg atgccgcctt ccgcgcggca ggcagcacca attccggcat
     5041 catcatgaac attccggtgg aagtgcagat cgttctcggc agcaccgaaa tgccggtctc
     5101 ggacctgatg tcgttgcaga agggttcgac cgtggcgctg aaccgccgca tcggcgagcc
     5161 ggtcgatgtc gtcgtcaacg gccgcaggat cgcgcgcggt gagatcaccg tcctggaaag
     5221 cgatccttcc cgcttcggca tcaggctgac cgagatcgtc gctgcggcga agagcgctta
     5281 agcatggacg cggcggtgca ttgcatgggg aaagcagcgg catgacgcag ttggtcctga
     5341 cgcgcgcgca aaaagccgcc gccatcctcg tggcgatggg caagccatcg gccagccgcc
     5401 tgctgaaatt cttcaagcag gaggaactga aggcgctgat cgaaggcgcg cgcctgctgc
     5461 gcaccattcc gcaaagcgac ctggaacgca tcgtcacgga attcgaggcc gagttcaccg
     5521 agggcgcggg cttgctcgat tccgccgaca agatggacac catcctcaat gaatcgctgt
     5581 cgccggaaga agtgagcgcc atcatggccg gcaaacagcc ggaggccgca cagcccggac
     5641 cgccgccgat ctggcccgat ctggagaagc ttgagcctgc acggcttggc gccttcctcg
     5701 ccggtgagca tgcacagacc gccgccatgg tgctgtcgaa gctttcgccg caggccgcag
     5761 ccagcgtgct gcttacactc gacaagccgc tgcgtaatac ggtcatcgga cggatggtga
     5821 ccttgtcggc cgtgccggaa acggcgacga agatcgtcga aaaccaactg cgcatgcgcg
     5881 tgatgggcga aacctcggcc aaggacaatt ccgccggaca ggtgcgcgtc gccagcctgc
     5941 tcaacgaaat ggacaagtcc cagctggacg aggtgatgca ggatctggaa caatccggca
     6001 cgcccgatct ggcggcgatc cgggcgcggc tattctcctt cgaggatatc ctgcttttga
     6061 cgcaaaaggc gcgcgtcgcc ctgttcgacg ggctttccag cgagcaggtg acactgtcgc
     6121 tgcgcggcgc gccggcggat atggtcgaag ccgtgctttc ctccatcggc gcgcgctcgc
     6181 gccgcatgat cgaatccgaa ctcggccagg ccgcggatgg cgtcgcgccg gcggatatcg
     6241 tgcaggcccg caagaccatc gccacgacgg cgatccgcat ggcgcgcgag ggagccttcg
     6301 agcttccgtc gacacagacc gccgaagcgg cgtagcgggc gcggtcaagc catggccgat
     6361 gcggtcgaca aggactcgaa gaccgaggaa gcgaccgaaa agaagattcg cgacacgatc
     6421 gagcagggca agctgccgca ttcgcgcgaa accgccctgc ttgcctcgtt cgtcgctatc
     6481 ctgatctaca ccgtgttcta cgccaaggac gctttcgtcg acctcggctc gttcctgtcg
     6541 atgttcctcg aaaagccgga agcctggccg atggacagtg aaacggatgt catcgagctt
     6601 tacaagaccg tgatgatggc caccacgcgc gccgtcacca gcttgctggt gctcctggtc
     6661 atcgccggcg tcggctcgtc ggtgttccag aacataccgc aattcgtggc cgaacggatc
     6721 acgccgcagc tttcgcgcat ctcgatcgcc aaaggctgga gccggctgtt cggcgtgcag
     6781 ggcttcgtcg aattcctgaa atcgctggcg aaggtcggga ttgcctgcac ggttctggtc
     6841 ttcacgctgt cgcaggacca ccgccgcctt ttggcgggca tgatgaccaa tccgctcgaa
     6901 ttcccgatgg tcatccgggg catcacggtc gatattctgg tgacgatcgt gttcgtcatg
     6961 gcgctgatcg ccggcgttga tcttgtctgg tcccgcttcc actggaagca ggaaatgatg
     7021 atgagcaagc aggaagtgaa ggacgagttc aagcaatcgg aaggcgatcc catcatcaag
     7081 tcgcggcttc gttcgctggc gcgagaccgg gcgcgcaagc gcatgatgac ggcggtgccg
     7141 cgcgccacgc tgatcatcgc caacccgacc cacttctcga tcgcgctcaa atatgtccgc
     7201 gaggaggatt ccgccccgct ggtgctggcc aagggccagg atctggtggc gctgaagatc
     7261 cgcgagatcg ccaaggaaca caacattccg atcttcgagg acgtggcgct tgcccgctcc
     7321 atgtacaagc aagtttcggt ggatagtgtg atcccgtcgc agttctacca agccgtcgcc
     7381 gagctggtgc gggttgttta ttccaggaaa gccggacgca aacaggtttc atgaacgaac
     7441 ggtcgatcgc ataatgaaca gacagcccca cgcaagcacg cgcgagatca tcgtggccgg
     7501 cgccatcgaa caggtggtgc aggaattgcg gcttgtcgat gtggccgact acatcgcctt
     7561 catccggctg gagcatttcg cctgcttgtc cgatctcgtg gattcggcgg cggagttgtt
     7621 cttcctgccc ggcacgctga ggctaggcca cggcggcgag gcccatgtgg actggggcag
     7681 cagtccgcgc atcgagcttg acctggaact gcgcccgaaa ggcgcaaccg tctacttccg
     7741 gcttaccctg tccgaacacg gtgcatcggt ggtggtcaat tacgtatcgt tcgaggagcc
     7801 ggacgaagac ccctcccgca acaccgcgct attggaagcg gccatcgaac aggcgcgcat
     7861 gcgccgttcc gagccgctgg cgtttcgctg aattctcact cgatcagccc caacctgagc
     7921 gccttggcaa cggcttgagt gcggctgacg gcattgagct tctgcgcgca ttggctgaga
     7981 tactggttgg cggtatggac cgacagcttc agcagccgcg caatcgcttc gctggtatag
     8041 ccgttggcgg tcagcttcag gcattccagt tcgcgcttgg agattgcccg cgtccggcct
     8101 gaatcgccgg gcctcgtgcg ggcgacggct gagaacagcg aaaagcagcg ggcatggatt
     8161 tcgtagagca ggtcctgcga cggtgcgatt tccgatccga ggaacaccgc cagcccgcat
     8221 tggccgcgtt cggcatggac gggaaaggcg atgccgttgg tgccgggcac cagcggcgtc
     8281 atcgcctcgg tccaggcaag gttgcggaac gccgcgtccg aacccggcac gccgtcgtcg
     8341 gcccaccagc gcggttcggt cgaaacgccg gtatggcgca ccatatcctc gccattggcg
     8401 ccggacatga atttcgtcgc taccgcgatg ctcggatatt cattgtcgaa gcaggggacg
     8461 aggcgcgcgc gttcgggcga gggacttacg aaaaacaggc cgaagcccga ggcgtgaaga
     8521 tcgacggcga tccagcggca gcggcgcacg gcatcgggaa tggtgatgat ctgatgggtt
     8581 tccgaaccga gcgtgaacgc gccgaacggg ttgcgatgtt cgttgaacag cgcctcggcg
     8641 gcttccctga tttcggcttg tgcggcaaat gcgtttttca aatgatgccg ctcctgattg
     8701 cggttgcaat cgccatcgcc cggttggagg ccgaaaactt ctggattgcg tgggtgatgt
     8761 agctgttgac ggtgttggac gagacgccga ggatgacggc gacttcatcg gtggtcttgc
     8821 cttccgaaac ccagaacaga cattcgcgct cacggtcgga aagcgggtcc tgcatggcgg
     8881 cttgcgccag caattgcggg atttgcgaaa gcgcgtagca gcatttcagc tgcgccttca
     8941 tcagttcggt ggtgtcgatg ccgcctgcct tggccgaaga aaacagcacc gacagccgct
     9001 ggcgcccgac attcaaccgc aaggaataaa tctccgaatg gccaagcacg tcgagaaggc
     9061 gggcttcctc gccgctgacc aggccgacgg catcgggcgc ttcgcttgcc gtcagcgggc
     9121 gaggccggct gcccggcgct gccgtcagca tcccttgcgc gaggcctgcg atcagccgtt
     9181 tgccaatcag ttcgatggcg tcgaaaatcc agttggagga aacgatgcgc gcatcgttct
     9241 tgtcctggtc atggacgatg gcgaccagca tgtagctgtc ggcgccgatt tcggcgcaca
     9301 ggttcatgaa gaagccggtc aggtcgccac ggcatgaaat atgtgttctc ggcccatcgg
     9361 aggggtcttt gtcgagaagc gcaacgggac cgttcatttt gtcatcaggc cggaatcggt
     9421 ttccatgtcc gaggccggcg ttaccggtaa cggactttgc tacgcacaac ttttacgatt
     9481 gcacacgcca agcacgtaac cgtgcccgga tcaggcccga atccgtgagg gccgcaggac
     9541 catcgcacac cggcagccgg aaagcccggc accaggttcg agtggcagcc gcgcccccca
     9601 cggaccggct gattcgtcgt aatttacgcg cactcgaatc cgacttcaaa cacgaattaa
     9661 cgaattgtta actatgtgac t
//
LOCUS       DASYCS010000280         2568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3116847,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000280 DASYCS010000000
VERSION     DASYCS010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3116847"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..417)
                     /locus_tag="VGM46_08850"
     CDS             complement(103..417)
                     /locus_tag="VGM46_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEY4192742.1"
                     /translation="MTGTLTRADLTESVFQAVGLSRTDSAQMVEDMLEEICGALAKSE
                     TVKLSSFGTFAVRQKSQRMGRNPKTGDEVPIAPRRVLVFRPSHVLKAVINGQTVAPEA
                     ED"
     gene            complement(529..1500)
                     /locus_tag="VGM46_08855"
     CDS             complement(529..1500)
                     /locus_tag="VGM46_08855"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014761723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEY4192743.1"
                     /translation="MIRARVIGCGACLPDNVVTNSDLAKKVDTSDEWIRERTGIIQRY
                     IAPEGQNTSDLALGAGRAALTDAGIDAGELDMVIVATTTPDRTFPSVGTMVQSRLGMS
                     RGAAFDVQAVCSGFIYGLSLADSLIRTGQARTILLIGAETMSRVLDWNDRTTCVLFGD
                     GAGAVVLQAHDGKGDNGDQGILNSRIFSDGRLHDLLYTDGGVSSTRNAGLLHMQGREV
                     FKHAVTNIAAAIEASAAAAGIPVSDVDWFVPHQANQRILDGTAKRLGIDAAKVVSTVA
                     MHGNTSAASVPLALVTAVQDGRIKRGDLVLLEAMGAGFTWGACLLRW"
     gene            complement(1497..>2568)
                     /gene="plsX"
                     /locus_tag="VGM46_08860"
     CDS             complement(1497..>2568)
                     /gene="plsX"
                     /locus_tag="VGM46_08860"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEY4192744.1"
                     /translation="EDTNLPERVRVARELTISIDAMGGDAGPEIVVAALLRSLERHPG
                     TGVLLHGDEAILAPLLACTPKLKNRVTLRHAPERVTMEDKPSQIVRRGQNTSMWRAIE
                     CVKKGEAQAAISAGNTGALMAMSMLQLRTIEGISRPAIASIWPTMRGRTVVLDVGANV
                     TATAEQLVDFAVMGEAFAHAVLNLERPVVGLLNVGSEEMKGNDAVRGAAHILRNAKLP
                     IAFHGFVEGNDITQGVVDVVVTDGFTGNIALKTAEGAAKLVSQYLRQALRRSLLGMVG
                     AFFSQGALNTLRRNLDPRASAGGIFLGLNGVVVKAHGGTDAIGFAGALDMAIDMAQAD
                     INARIAADRGAIEPALEQAELT"
BASE COUNT          463 a          838 c          817 g          450 t
ORIGIN      
        1 cagcacatgc tgcggcagat cgaggtcgcc cgccgcttcg ctgatggagc ggaaggcctc
       61 cggcgatttt tcaacgaatg cgaaagccgg tgacgccaag actcaatcct ctgcttcggg
      121 tgcgaccgtc tggccgttga tgacggcctt caatacatga ctcgggcgaa acaccagaac
      181 gcgccgtggc gcgatcggaa cttcatcgcc ggtcttggga ttgcggccca tgcgctggct
      241 tttctgccgt acggcgaacg tgccgaagga ggacagcttc accgtttcgc ttttggccag
      301 ggcgccgcaa atctcttcca gcatgtcctc gaccatctgg gccgagtcgg tgcgcgaaag
      361 gcccaccgcc tggaagaccg actcagtgag atcggcacgc gtcaaagttc ccgtcatcgc
      421 cccgcgactc caaactatcc acaggagcct aggactgacc ggaaaggccg tcaatatcaa
      481 ttagatagga gaaaagcctc attctccgta cacggcggcc ttgcagggct accagcgcaa
      541 caggcaggca ccccaggtaa acccggcccc catggcctcc agcagaacca ggtcgccccg
      601 cttgatccgc ccatcctgaa ccgccgtcac caacgccaac ggcaccgaag cggcggaggt
      661 attgccgtgc atggcgactg tcgagaccac cttggccgcg tcgatgccca gccgcttggc
      721 ggtcccgtcc aggatgcgct ggttggcctg gtggggcacg aaccagtcga cgtcggagac
      781 aggaattcca gccgccgctg ccgaagcctc gatcgctgcg gcgatgttgg tcacggcgtg
      841 cttgaaaact tccctgccct gcatgtgcag cagcccggcg ttgcgggtcg aagagacacc
      901 gccatcggta tagagcagat cgtgcagccg cccgtcggag aagatgcggc tgttgaggat
      961 gccctggtcg ccattgtcac ccttgccgtc atgggcttgc agcaccacgg ccccggcgcc
     1021 atcaccgaac aggacgcagg tggtacggtc gttccagtcc agcacccggc tcatggtctc
     1081 ggcgccgatc agaaggatcg tgcgggcctg accggtgcgg atcaggctgt cggccagcga
     1141 caggccgtag atgaagccgg aacagacggc ctgcacatcg aaggcggcgc cgcggctcat
     1201 gcccaggcgc gactgcacca tcgtgccgac ggaaggaaag gtgcgatccg gcgtcgtcgt
     1261 ggcaacgatg accatatcca gttcgccggc gtcgatgccc gcatcggtca gcgcggcgcg
     1321 gccggcgccc agcgccagat cggaggtgtt ctggccttcc ggcgcgatgt agcgctggat
     1381 gatgccggtg cgctcgcgaa tccattcgtc ggaggtatcg acctttttcg ccaggtcgct
     1441 gttggtgacg acattgtccg gcaagcaagc gccgcagcct atgacacggg cccggatcat
     1501 gtcagttccg cctgttccag cgccggttcg atggcgccgc ggtcggcggc gatgcgcgca
     1561 ttgatatcag cctgggccat gtcgatggcc atgtcgaggg caccggcaaa tccgatggcg
     1621 tcggtgccgc catgcgcctt taccaccacg ccattgagcc cgagaaagat gccgcccgcc
     1681 gaggcgcgcg gatcgagatt gcgccgcagc gtgttcagcg cgccttgcga gaagaacgcg
     1741 ccaaccatgc ccagcagcga ccgcctgagg gcctggcgaa gatattgcga aaccagcttg
     1801 gcggcgccct cagcggtttt gagggcgata ttgccggtga agccgtcggt caccaccaca
     1861 tcgaccacgc cctgggtgat gtcgttgcct tcgacgaaac cgtgaaaggc gatcggcagt
     1921 ttcgcgttgc gcaggatgtg ggcggcgccc cgcaccgcgt cgttaccctt catttcctcg
     1981 gagccgacat tcagcaatcc gacgaccggc cgctctaggt tgagcacggc atgggcgaag
     2041 gcttcgccca tcacggcgaa atcgaccagt tgttcggcgg tggcggtgac attggcgccg
     2101 acatccagca ccacggtgcg tccgcgcatg gtcggccaga tcgacgcgat ggcgggacgc
     2161 gagatgccct cgatggtgcg aagctgcagc atgctcatcg ccatcagcgc cccggtgttg
     2221 ccggccgaga tcgccgcctg ggcttcgcct ttcttgacgc attcgatggc ccgccacatg
     2281 gacgtgttct ggccccggcg cacgatctgg ctgggcttgt cttccatggt gacgcgctcg
     2341 ggcgcatggc gcagcgtcac acgatttttg agcttggggg tgcaggccag caagggcgcc
     2401 agtatggctt catcgccatg cagcagcacg ccggtgcccg gatgccgttc cagcgaacgc
     2461 aacagcgcgg ccacgacaat ttccgggccg gcatcgccgc ccatggcatc aatcgagatt
     2521 gtcaattcgc gcgccacgcg aaccctttcc ggaagattcg tgtcttca
//
LOCUS       DASYCS010000281         1906 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4998121,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000281 DASYCS010000000
VERSION     DASYCS010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4998121"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="VGM46_08865"
     CDS             <1..739
                     /locus_tag="VGM46_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEY4192745.1"
                     /translation="TAGVPRKPGMSRDDLLGINLKVMEQVGAGLKKYAPKAFVICITN
                     PLDAMVWALQKFSGLPKSHVVGMAGVLDSARFRYFLAEEFKVSVEDVTAFVLGGHGDS
                     MVPMIRYSTVSGIPLPDLVKMGWTTQEKLDQIVQRTRDGGAEIVGLLKTGSAYYAPAA
                     SAIQMAESYLKDKKRVLPCAANLSGQYGLKDTYVGVPVVIGAGGVERVIEIDLDKAEQ
                     KMFDASVATVKGLTDACTKIAPQLAQK"
     gene            756..>1906
                     /gene="sucC"
                     /locus_tag="VGM46_08870"
     CDS             756..>1906
                     /gene="sucC"
                     /locus_tag="VGM46_08870"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002964994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEY4192746.1"
                     /translation="MNIHEYQAKALLKGFGAPVAEGVPVFKASEAEAAAKALPGPLYV
                     VKSQIHAGGRGKGKFKELSADAKGGVRLAKTVAEVVANTNEMLGSTLVTKQTGPAGKQ
                     VNRLYIEDGADIERELYLSILVDRTVGRVAFVVSTEGGMDIETVAHDTPEKIVTVAID
                     PEKGVGTDDIAKINAALKLSGDAAKDGEKLFPILYKAFVEKDMSLLEVNPLIVMKDGR
                     LRVLDAKVSFDNNALFRHPDILELRDTTEEDAKEIEASKYDLAYVALDGNIGCMVNGA
                     GLAMATMDIIKLYGAEPANFLDVGGGATKEKVTAAFKIITADPSVKGILVNIFGGIMK
                     GDVIAEGVVAAVKEVGLNVPLVVRLEGTNVALGKKIIDESGLNVISADDL"
BASE COUNT          385 a          613 c          593 g          315 t
ORIGIN      
        1 caccgccggc gtgccgcgca agccgggcat gagccgcgac gacctgctcg gcatcaattt
       61 gaaagtgatg gagcaggtgg gcgctggcct gaagaaatac gcgcccaagg ccttcgtcat
      121 ctgcatcacc aacccgctcg acgccatggt gtgggcgttg cagaagttct ccggcctgcc
      181 caagagccac gtcgtcggca tggccggcgt gctggattcg gcccgcttcc gctacttcct
      241 ggcggaggag ttcaaggttt cggtcgagga cgtcaccgcc ttcgtgctcg gcggtcatgg
      301 cgattccatg gtgccgatga tccgctattc gacggtttcc ggcattccgc tgcccgacct
      361 cgtcaagatg ggctggacca cgcaggaaaa gctcgaccag atcgtgcagc gcacccgtga
      421 cggcggcgcc gagatcgtcg gcctgctcaa gaccggctcg gcctattacg cgccggccgc
      481 ttccgccatc cagatggccg agagctacct caaggacaag aagcgcgtac tgccctgcgc
      541 cgccaacctt tccggccaat atggcctgaa ggacacctat gtcggcgtcc cggtcgtcat
      601 cggcgccggc ggcgtcgagc gcgtcatcga gatcgacctc gacaaggccg agcagaaaat
      661 gttcgacgcc tccgtcgcca ccgtgaaggg cctgaccgac gcctgcacga aaatcgcgcc
      721 gcaactggcc cagaaataaa ccggaggctt tgccgatgaa catccacgag tatcaggcca
      781 aggcccttct gaagggtttt ggcgcaccgg tggccgaggg cgtgccggta ttcaaggcca
      841 gcgaggcgga ggccgccgcc aaggcgctgc ccggtccgct ttatgtcgtg aaaagccaga
      901 tccacgccgg cggccgcggc aagggcaaat tcaaggaact gtcggccgac gccaagggcg
      961 gcgtgcggct ggccaagacg gttgccgaag tcgtcgccaa caccaatgaa atgctcggtt
     1021 cgacgctggt caccaagcag accggcccgg ccggcaagca ggtcaaccgc ctctatatcg
     1081 aggacggcgc cgatatcgag cgcgagcttt atctgtcgat cctggtcgac cgcaccgttg
     1141 gccgcgtcgc cttcgtcgtt tcgaccgagg gcggcatgga catcgagacg gtcgcgcacg
     1201 acacgccgga aaagatcgta accgtggcga tcgacccgga aaagggtgtc ggcaccgatg
     1261 acatcgcgaa gatcaatgcc gcgctgaagc tttcgggcga cgcggccaag gacggcgaaa
     1321 agctgttccc gatcctctac aaggccttcg tcgagaagga catgagcctg ctcgaagtca
     1381 acccgctgat cgtcatgaag gatggccgcc tgcgcgtgct cgacgccaag gtgtcgttcg
     1441 acaacaacgc gctgtttcgc caccccgaca ttcttgaact gcgcgacacc accgaagaag
     1501 acgccaagga aatcgaggcg tcgaaatacg acctcgctta tgtcgcgctc gacggcaata
     1561 tcggctgcat ggtcaacggc gccggccttg ccatggcgac gatggacatc atcaagcttt
     1621 acggcgcgga gcccgccaac ttcctcgacg ttggcggcgg cgcgaccaag gaaaaggtca
     1681 cggcggcgtt caagatcatc accgccgatc cgtccgtcaa gggcatcctc gtcaacatct
     1741 tcggcggcat catgaagggc gacgtcatcg ccgaaggcgt ggtcgccgcg gtcaaggagg
     1801 tcggcctcaa cgttccgctg gtggtgcgcc tcgaaggcac caacgtcgcg cttggcaaga
     1861 agatcatcga tgaaagcggc ctcaacgtca tttcggccga cgacct
//
LOCUS       DASYCS010000282         3964 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1231101,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000282 DASYCS010000000
VERSION     DASYCS010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1231101"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="VGM46_08875"
     CDS             complement(<1..623)
                     /locus_tag="VGM46_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2497 domain-containing protein"
                     /protein_id="HEY4192747.1"
                     /translation="MATASSVQREPSMEEILASIRRIIEDSDTGRKQPDEADELRQDL
                     APAPGAVRGTDMDAFRAELHAGVESRRPATLAEVQPQPSAEPATARMEPPARSVPVKA
                     PATLAEVSARIAAEPVVPVARADAPMIAEATGTARAAGISGKMDGIVADWRRELPGVG
                     EQAKAAVPMQEPAAESKIECLEDLATEPAFEPQPSHGSAEAVTAEMPV"
     gene            complement(817..2229)
                     /locus_tag="VGM46_08880"
     CDS             complement(817..2229)
                     /locus_tag="VGM46_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006335248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HEY4192748.1"
                     /translation="MSRISKSVFAAILVSATALSPIAASAETIAGALSKAYQNNSQLN
                     SARAGVRVTDEGVAIAKSGYRPTVIGSGSIDYSSTRGPSAAFGGANNTTRLTTGGFGV
                     QINQTLFDGFQTKNNVAAAKSQVSASVETLRNTEENILLNAATSYMDVIRDRQVAVLT
                     EQNLQFLTEQARAARSRFEVGEGTRTDVAQADASRSAAVAKLSAARAQALSSAAIYHQ
                     IVGDEPGKLKAAAPVGKLLPSSIDAAVAIASNEHPAILATQHLVDAAAFSVKSAEGAL
                     LPQLSASAGVERDYRNSDGNGAALAGTSNGYSNSASIGATLTVPIYQGGRASAQVRQS
                     KESLGQARIEVDVSRDNVRAAVTSAWTQYTASREGVDANRALVAAAQLALNGVIEERN
                     VGQRTTLDVLNSQNDLITAKINLVSSERDVVVASYAILSAIGHLSTERLGLQVTKYRP
                     EEHYNAVKDKWIGLRTPDGR"
     gene            complement(2400..3068)
                     /locus_tag="VGM46_08885"
     CDS             complement(2400..3068)
                     /locus_tag="VGM46_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEY4192749.1"
                     /translation="MNADFSELRVKMVDGQIRTTDVTSIPLLEALLSVPREAFVPAEK
                     QDVAYIDEDIRIATGPEGPRYLMEPSPLAKLLQITEVSAGDRALVVGCGTGYSAAILS
                     RLAKSVVALESDAALCELARTTLGELGCGNVTVVNGALPEGHAANAPYDVIFVDGSVE
                     ALPKSLSDQLAEGGRLVAVEGHGNAGIARLFVKTADAVSGRRAFNAAIKPLPGFERAE
                     AFVF"
     gene            complement(3152..3225)
                     /locus_tag="VGM46_08890"
     tRNA            complement(3152..3225)
                     /locus_tag="VGM46_08890"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3191..3193),aa:Cys,seq:gca)
     gene            complement(3430..3693)
                     /locus_tag="VGM46_08895"
     CDS             complement(3430..3693)
                     /locus_tag="VGM46_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015317514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192750.1"
                     /translation="MTAGNDGDAAPARKISVFGREFKMPRSRWARIAIGLALTILGLF
                     GFLPILGFWMVPLGLLVLSYEFASVRRLRRRFVIWWGRRRTRS"
BASE COUNT          795 a         1224 c         1190 g          755 t
ORIGIN      
        1 accggcatct cggcggtcac ggcttcggcc gagccatggg acggctgcgg ctcaaaagca
       61 ggctcagtcg ccaaatcctc gagacattcg atcttcgatt cggctgccgg ctcctgcatc
      121 ggcacggcgg ctttcgcctg ttcgccaaca cctggaagct cacgccgcca atcggcaacg
      181 atgccatcca tctttccaga aatccccgcg gcccttgcag tcccggtggc ctcggcaatc
      241 attggcgcat cagccctggc aaccggcacg acgggctcgg ctgcgatccg cgcgctgacc
      301 tcggcaagcg tcgccggcgc cttcactgga acggaacgcg caggaggttc catgcgggcg
      361 gtggcaggtt cggcggacgg ttgcggctgc acctccgcca acgttgctgg cctgcggctc
      421 tctactccgg catgaagttc ggcgcggaat gcatccatat ccgtgccgcg gacggcgccc
      481 ggcgcaggcg cgagatcctg gcgcagttca tcggcttcat caggttgctt gcggcctgtg
      541 tcgctgtctt cgataatccg cctgatggaa gccagtatct cttccattga aggttcgcgc
      601 tgtacgctgc ttgccgtcgc catcgtcaca tccgccgcct ttgtgacccg tttcagattt
      661 tcgttccggc cgaacccgga ttcgaatcat ggcgacagcg tagccgacgg aacgccgcac
      721 gagaatcccc aatcggggga cttgtgggat ttcaacagat tagagcagat cccgaaccag
      781 gaatcggcca agccaaaacg gcagccggct tgcggctcag cggccgtccg gcgtgcgcag
      841 gccgatccac ttgtccttga cggcgttgta gtgctcttca ggccggtact tcgtcacctg
      901 gaggccaaga cgttcggtcg agagatgacc gatagccgaa agaattgcat agctcgccac
      961 cacgacgtcg cgttccgaac tgacgagatt gatcttggcg gtaatcaagt cgttctggga
     1021 attcaacacg tcgagcgtgg tgcgctggcc gacattgcgt tcctctatca cgccattgag
     1081 tgcgagttgc gcggcggcga ccagcgcgcg gttggcgtcg accccttcgc gcgaggcggt
     1141 gtattgcgtc catgccgaag tcacggcggc gcgaacgttg tcgcggctga catcgacttc
     1201 gatcctggcc tggccgagcg attctttcga ctggcgaacc tgcgccgaag cgcggccgcc
     1261 ttgatagatc ggcaccgtga gggtggcgcc gatgctggct gaattggaat aaccattcga
     1321 tgtgccggca agtgccgcgc cattgccatc cgagttacga tagtcgcgct cgaccccggc
     1381 agatgcggaa agctgcggca gcaacgcgcc ttccgcggat ttgacggaga aagcggcggc
     1441 atcgacaagg tgctgggtag ccagaattgc cggatgttcg tttgatgcaa tggcgactgc
     1501 ggcatcgatg ctggaaggca gaagcttgcc gaccggggcg gcagccttca acttgcccgg
     1561 ctcgtcgccg acgatctggt ggtagatcgc agcgcttgaa agtgcctgcg cgcgtgcggc
     1621 gctgagcttg gcgaccgccg ccgagcgtga agcatcggcc tgggcaacgt cggtgcgggt
     1681 accctcacct acctcgaaac gcgagcgtgc ggcccgcgcc tgttcggtca agaactgcaa
     1741 attctgctcg gtgagcacgg cgacctggcg gtcgcggatc acatccatgt aggacgtggc
     1801 ggcattgaga agaatgtttt cttccgtgtt gcgcagggtt tcgactgaag cggaaacctg
     1861 agacttggcg gcggcaacgt tgttcttggt ctgaaagccg tcgaaaagcg tctgattgat
     1921 ctgtacgccg aagccgcccg tagtcagtct cgtcgtgtta tttgccccgc caaacgcggc
     1981 cgacggcccc ctcgtgctcg aataatcgat gctgccggag ccgataacgg taggccggta
     2041 gcccgacttg gcgatagcca cgccttcatc ggtgacgcgg acgccggcac gggccgaatt
     2101 gagctgcgaa ttattctgat aggccttcga caatgcgccg gcgatggttt ccgccgatgc
     2161 agcgatcgga gaaagcgccg ttgcagacac aaggatggcg gcaaaaactg atttgcttat
     2221 acgcgacacg acgtatccct cactttggtc atggaaacta cgccgcggca actgcttcta
     2281 accagccggc gcgacgtttt tcagtttcct gctgctggcg cacgccccgc gtatcggtca
     2341 gcagcgattc catggtggga agcgaccacg accgcgctgc ggctgcaagt caaaaacatt
     2401 cagaatacaa aagcttcggc acgttcaaaa ccgggtagtg gctttattgc cgcattaaat
     2461 gcccgccgac cgctcaccgc atcggccgtt ttcacgaaaa gccgcgcaat gccggcattg
     2521 ccatgtcctt cgacggcgac cagccggcca ccttcagcaa gttgatccga caggctcttc
     2581 ggcagcgcct cgacggagcc atccacgaaa atcacgtcat agggcgcgtt cgccgcatga
     2641 ccctcgggca gcgcaccgtt gacgacggtg acgttgccgc aaccaagttc gcccagcgtc
     2701 gtcctggcaa gttcgcaaag ggcggcgtcg ctttccaacg ccacgacgga tttggccagg
     2761 cgcgaaagaa tggcggcgga atatcccgtg ccgcaaccaa cgaccaatgc ccgatcgccg
     2821 gcggaaacct cggtgatttg caggagcttg gccaatggcg aaggctccat cagatagcgc
     2881 ggaccttcag gcccggtcgc gatgcggata tcctcgtcga tataggcgac gtcctgtttt
     2941 tcagccggaa cgaacgcttc gcgcggcacc gacagcaagg cctccagcaa cggaatgctg
     3001 gtgacatcgg tggtgcgaat ctggccatcg accatcttga cgcgaagttc ggagaaatca
     3061 gcgttcattt tgccgccttt ttgcctgcgc gacagtgccc gacttaattc gattcgatcc
     3121 cgcccgcgca ggcaggccgg ggtccggatt ttggaggcct cgcccggaat cgaaccgggg
     3181 tgcaaggatt tgcagtcctc tgcgtaacca ctccgccacg aggcctaaac tcaaaacccg
     3241 tttggattca gagatttacg aaggaacaac ctgccgcctc gaaaaccacc ttcttcgccg
     3301 ttccgaatag acgttctttt tcagtccttc aaggggcgca aacaaaaaag ccatcttcgc
     3361 cacaaccgat cgaaggctga accacgcgct ggaaaatcgc ggacgcaccc gtgcccgcca
     3421 ctacgcagcc taggaccgcg tgcgccgccg cccccaccag atgacgaaac gacgcctgag
     3481 ccgccgcacc gatgcgaact cataggaaag caccagcaat ccgagcggca ccatccagaa
     3541 acccaggatg ggcaggaagc cgaagaggcc gagaatggtc agggccaagc cgatggcgat
     3601 tcgcgcccag cgcgaccggg gcattttgaa ctcgcggccg aaaacggaaa tcttgcgtgc
     3661 cggcgcagcg tcgccgtcat ttcctgcggt catattcaac ttcttatccg gcttttccag
     3721 atcgatcgct ttcaggcaat gaggtccgcc atgagtacgg caagcagcta tataagaggc
     3781 gccgatggga gattgcgacc attggccgaa aaaagaccga gaaaaatgcg aaatgcttct
     3841 ttgcaaagct gaaacgggtt tgctataggc tgcctcgcac gacaagagcc cccgttcccc
     3901 ggtagctcag tggtagagca accggctgtt aaccggttgg tcgctggttc gaatccggcc
     3961 cggg
//
LOCUS       DASYCS010000283         2083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4130666,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000283 DASYCS010000000
VERSION     DASYCS010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4130666"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1874)
                     /locus_tag="VGM46_08900"
     CDS             complement(<1..1874)
                     /locus_tag="VGM46_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY4192751.1"
                     /translation="MSASFRIPNAGRLKQGKPVQFTFDDKAYAGVEGDTLASALLANG
                     VHLVGRSFKYHRPRGILSAGAEEPNALVGIERDAARKAPNVRATVQEIYDGLKAGSQN
                     RWPSLSFDIGAVNDIASPFFSAGFYYKTFMWPKSAWKSFYEPKIREAAGLGVAPDKAD
                     PDHYAAQYEHCDVLVLGGGAAGIAAALAAAETGARVVIADEQAELGGSLRFESGARID
                     GQDGWSWAQAMVARLDAMANVRVLPRTTAFGYYAQNFIGLTERVSDHLAKPGADLPRE
                     RLWQIRAKRVVLATGSIERHMVFADNDRPGVMLASAARIYLNHYGVAVGRNVGVYTAN
                     DSAYAAAIDLKKAGVNIAALVDLRDNPTGELIDEARALGIEVHTGRAVIRAGGKLRVS
                     SMVVQPKNGGGERRIAVDAILMSAGWTPSVHLFSQSRGKVAFNDEVKRFLPGAYAQDC
                     VSVGACNGTDGLSATIDEACAAGAKAARDAGAKAAKSTKPKVDASESWSRGMLGSAPG
                     AGPGTTVKAFVDFQNDVTAKDIRQAVHEGMRSIEHVKRFTTNGMATDQGKTSNMHGLA
                     IAADELAKPIPQVGLTTFRAPYTPVTFGSIVGHARGALFDPTRRTATHGWAAAHGAVF
                     "
     gene            complement(1871..>2083)
                     /locus_tag="VGM46_08905"
     CDS             complement(1871..>2083)
                     /locus_tag="VGM46_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HEY4192752.1"
                     /translation="SAHIAEESDADFEKFFFIRTNPKGIIYERWRHVHGCARFFNAVR
                     DTITDKFVMTYKAGEPKPTKLPGAGK"
BASE COUNT          344 a          701 c          666 g          372 t
ORIGIN      
        1 tcgaacaccg cgccatgcgc ggccgcccag ccatgcgtcg ccgtgcgccg cgtcgggtcg
       61 aacagcgcgc cgcgcgcatg cccgacgatg gagccgaacg tcaccggcgt atagggcgcg
      121 cggaacgtgg tcaggcctac ctgcgggatc ggcttggcca gctcgtcggc cgcaatcgcc
      181 aggccgtgca tgttggaagt cttgccttgg tcggtcgcca tgccgttggt agtgaagcgc
      241 ttgacgtgct cgatggagcg catgccttca tgcaccgcct ggcggatgtc cttggcggta
      301 acgtcgttct ggaaatcgac gaatgccttg accgtcgtgc ccggaccggc gccgggagcc
      361 gatcccagca tgccgcgcga ccagctttcg ctggcgtcca ccttcggctt ggtgctcttg
      421 gccgcttttg cgccggcatc ccttgccgcc ttggcgcccg ccgcgcaggc ttcgtcgatc
      481 gttgccgaaa gcccgtcggt gccgttgcat gcgccgaccg acacgcaatc ctgcgcatag
      541 gcgccgggca ggaagcgctt gacttcgtcg ttgaaggcga ccttgccgcg cgactgcgaa
      601 aacaggtgca ccgatggcgt ccagccagcc gacatcagga tcgcatcgac ggcgatcctg
      661 cgttcgccgc cgccattttt cggctgcacc accatcgagg aaacgcgcag cttgccgccg
      721 gcgcggatga cggcgcggcc ggtgtgcacc tcgatgccca gcgcgcgcgc ctcgtcgatc
      781 agctcaccgg tcggattgtc gcgcagatcg accagcgctg cgatgttcac gccggccttc
      841 ttcaggtcga tagccgcggc gtaggcgctg tcattggcgg tatagacgcc gacattcctg
      901 ccgaccgcga cgccgtaatg gttgagatag atgcgcgccg ccgacgccag catgacgccc
      961 ggccggtcat tgtcggcaaa caccatgtgg cgctcgatcg agccggtggc cagaacgacg
     1021 cgcttggcgc ggatttgcca cagccgctcg cgcggcaggt cggcgcccgg cttcgccaga
     1081 tggtcgctga cacgctcggt caggccgatg aaattctgcg cataatagcc aaaggcagtc
     1141 gtgcgcggca aaacccgcac attggccatg gcgtcgagcc tggccaccat cgcctgcgcc
     1201 cacgaccagc catcctgccc gtcgatcctc gcgccgcttt cgaagcgaag cgacccgccc
     1261 agttccgcct gctcgtcggc gatgacgacg cgcgcgccgg tttccgccgc tgccagcgca
     1321 gcagcgatgc cggccgcgcc gccgcccagc accagcacgt cacaatgctc gtactgcgcc
     1381 gcataatggt cgggatcggc cttgtccggc gccacgccga ggccggcggc ttcgcggatc
     1441 ttcggctcgt agaacgactt ccatgccgat ttcggccaca tgaaggtctt gtagtagaaa
     1501 ccggcggaga agaacggcga ggcaatatcg ttgaccgcgc cgatgtcgaa ggaaagcgac
     1561 ggccagcggt tctgcgaacc ggccttcagc ccgtcataaa tctcctgcac cgtggcgcga
     1621 acgttggggg ccttgcgcgc cgcgtcgcgc tcgatcccga ccagcgcgtt gggttcctcc
     1681 gcgcctgccg aaagaatgcc gcgcgggcgg tgatatttga acgaacgccc caccagatga
     1741 acgccattgg ccaaaagcgc cgatgccagc gtgtcgccct cgacgccggc ataagccttg
     1801 tcgtcgaagg tgaattgtac cggcttgccc tgtttgagcc ggccggcatt gggaatacgg
     1861 aacgatgcgc tcatttgccc gctcccggca atttggtggg tttcggttcg ccggccttgt
     1921 aggtcatgac gaacttgtcg gtaatggtgt cgcgcaccgc gttgaagaag cgcgcgcagc
     1981 catgcacatg ccgccagcgc tcatagatga tgcccttggg attggtgcga atgaagaaga
     2041 acttctcgaa atccgcatcg ctttcctcgg caatgtgcgc gga
//
LOCUS       DASYCS010000284        21417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3119411,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000284 DASYCS010000000
VERSION     DASYCS010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 21417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21417
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3119411"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="VGM46_08910"
     CDS             complement(<1..460)
                     /locus_tag="VGM46_08910"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEY4192753.1"
                     /translation="MTSPSEIKKQLRREALARRDGLDPFWRVEAALDMAGTARDKIAV
                     EPGQVVSGFWPMRSEVDVRPLMFALLENGARLCLPAILDKTTIVFRELVRGAPMVEMG
                     FGTIGPHEEAKLLDPQIMLVPLAAFDARGHRIGYGGGYYDRAIERLAKAGK"
     gene            complement(500..654)
                     /gene="ssrS"
                     /locus_tag="VGM46_08915"
     ncRNA           complement(500..654)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VGM46_08915"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            793..1632
                     /locus_tag="VGM46_08920"
     CDS             793..1632
                     /locus_tag="VGM46_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018429953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY4192754.1"
                     /translation="MRFEGTAAYVADKDLMVAVNAAIALERPLLVKGEPGTGKTELAR
                     QVAAALGLELIEWNVKSTTRAQQGLYEYDAVSRLRDSQLGDERFNDIANYIKRGKLWD
                     AFAAGRKIVLLIDEIDKADIEFPNDLLQELDKMEFFVYETGETIRAAVRPIVIITSNN
                     EKELPDAFLRRCFFHYIRFPDVETLHRIVEVHYPGIKQNLVRAALTQFYEIRDVPGLK
                     KKPSTSEALDWIRLLIADDIDPADLRADAKNALPKLHGALLKNEQDVHLFERLAFMAR
                     RQG"
     gene            1710..2156
                     /locus_tag="VGM46_08925"
     CDS             1710..2156
                     /locus_tag="VGM46_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009450879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY4192755.1"
                     /translation="MTDITVRSLEKSDHADWRRLWTSYLDFYETKLPEEIYATTWERL
                     FSGGEFEPKGLIALVDGKAVGLTHYLYHRSGWSLANTCYLQDLFADETARGKGVGAAL
                     IEAVRREAEKLGVDNVYWLTNEKNATARKLYDRVARHSGFIKYRLS"
     gene            2164..3351
                     /locus_tag="VGM46_08930"
     CDS             2164..3351
                     /locus_tag="VGM46_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006200161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY4192756.1"
                     /translation="MFIPFFLELKAAKIPVSLREYLSLLEGLEAGLVDYDAEGFYYLA
                     RAALVKDERHIDRFDRVFAHFFKGVEALAGEGAVDVPDLPEDWLRKLAEKHLSDEEKK
                     LVEALGGFDRLMETLKQRLEEQKERHQGGSKWIGTAGTSPFGAYGYNPEGVRIGQQES
                     RNRRAVKVWDKREFRNFDDGVELGIRNIKVALKRLRRWVREGAAEEFDLPGTIHATAE
                     HGYLDVRTRPERRNAVKLLMFFDVGGSMDEHIKSVEELFSAARAEFRQLDFFYFHNCV
                     YEHVWKDNRRRAETVSTFDLVHKYGADYKAVFVGDAAMSPYEIAQPGGSVEHWNKEAG
                     SVWLSRLLQQWPNAVWLNPEAEKNWGYTHSTGMIGELFGGRMFGLTLAGLEAATRQLS
                     RKH"
     gene            complement(3378..3626)
                     /locus_tag="VGM46_08935"
     CDS             complement(3378..3626)
                     /locus_tag="VGM46_08935"
                     /inference="COORDINATES: protein motif:HMM:NF039341.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6074 family protein"
                     /protein_id="HEY4192757.1"
                     /translation="MAELIPFPLPSRAALVRSIVDDLECVHGPAASVFWRTRIAGIVA
                     SMRASGLPDETIRSQILSLQDAVQSELCERGLRAKNQA"
     gene            complement(3768..3844)
                     /locus_tag="VGM46_08940"
     tRNA            complement(3768..3844)
                     /locus_tag="VGM46_08940"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3808..3810),aa:Arg,seq:cct)
     gene            complement(3923..5086)
                     /locus_tag="VGM46_08945"
     CDS             complement(3923..5086)
                     /locus_tag="VGM46_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014329578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HEY4192758.1"
                     /translation="MPILNRAAEMQEEVSGWRRHLHQTPELNFDVFQTAAFVTEKLKE
                     FGCDEVVTGLGRTGVVGIIRGRLGAGETIGLRADMDALPINEITGKLYASTVPNKMHA
                     CGHDGHTAMLLGGAKYLAETRNFAGSVAVIFQPAEEGGGGGNEMVKDGMMDRFGISKV
                     FGMHNMPTLPVGCFAIRPGPIMAATAEFTITVKGRGGHAAMPNGTIDPIVITSHLVTA
                     LQTIASRNADPVEALVVSVTKFHAGDAYNVIPEKAEIAGTVRTLKKEMAAMAEQRMRA
                     ICAGIATTFNAEIEIDYDANYPVTFNHAEETVFAGDVAAAVAGDAKVIRAMPPVMGGE
                     DFSYMLEARPGAFIFIGNGDTAGLHHPAYDFNDEAIPHGISYWVKIAETALAA"
     gene            5289..6422
                     /locus_tag="VGM46_08950"
     CDS             5289..6422
                     /locus_tag="VGM46_08950"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006328352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEY4192759.1"
                     /translation="MNSRRFPRLVLAGMVALPAFCAPVMANPSLLFDLKTGKVLEHRD
                     AFKRWYPASLSKLMTAYVTFRAIDAGDVRLDSPIKVTKHSAAEPPSKMGFKPGQVMRL
                     DNAMKMMLVKSANDIAMAIGENVGGSQAAFAERMNAEAQRLGMTGSHFVNPNGLFSTE
                     QYTTARDLGLLVMAIRNEYPQYASWFSIEGLALGKRTIPNYNLLVGRYPGADGMKTGF
                     VCESGFNQIGSATRNGRTLVAVVLGEQTAITRAEVAAKLLDQGFAIPTSAATTVAALP
                     SYGDAVSENNMREELCSKKPASAQSEAPVVAAKPKSPYQVKLVAPTLVAVSLGGATGP
                     LPKAMVGKVEYADVPTPSWRPDRPSPFGEDAVAAQGDQSAKGL"
     gene            6425..7546
                     /locus_tag="VGM46_08955"
     CDS             6425..7546
                     /locus_tag="VGM46_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006200262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY4192760.1"
                     /translation="MQGLPIPVSVLTGFLGAGKTTLLNRLLKDPALTDTAVIINEFGD
                     VAIDHLLVEQASDGVIQLSDGCLCCTVRGELVDTLADLVDRLQTGRIKRLARVIIETT
                     GLADPAPVLQAIMAHPVLVQAFRLDGVITIVDAVNGSASLDGHVEAVKQAAVADRIVL
                     TKTDLADDIRAVDALRLRLRGLNPNAALLDAGDPGTGVASLFDCGLYNPATKTADVRR
                     WLGEEAQHDHSPHGHEGPDHRGHEGHDHGHRHDARVKSHTLIHDGPVPLAAIEMFLDL
                     LRSTHGDHLLRMKGIIELSDDRSRPLVIHGVQKTLHPPIRLAAWPDEQRGTRLVLITL
                     DMPQDYVQRLFAAFTNTPAIDTPDRAALEENPLAIAGRR"
     gene            7564..7962
                     /locus_tag="VGM46_08960"
     CDS             7564..7962
                     /locus_tag="VGM46_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011652293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEY4192761.1"
                     /translation="MKKTEASEGRSPSQQIDARIAELGDWRGRTLAHIRALIMQADPE
                     AVEEWKWRGVPVWEHGGQICTGETYKSAVKLTFLKGASLPDPSGLFNASLEGNARRAI
                     DLHEGDEIDEDAFKTLICAAVALNMAKARK"
     gene            complement(7992..8456)
                     /locus_tag="VGM46_08965"
     CDS             complement(7992..8456)
                     /locus_tag="VGM46_08965"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEY4192762.1"
                     /translation="MNDARQACELRPSCEADYDAIAEIWHRSASLPGVGPAVMPTEAE
                     LRGRLDAEFAAGWQVTLACRGDEIVGFLAIRPAEAVLAELFVRPGSLGAGIGRALLRH
                     AMAVMPDGFTLHTRATNERACRFYEKAGLTVLRHGVHPRSGDPLTYYGWNTG"
     gene            complement(8573..8845)
                     /locus_tag="VGM46_08970"
     CDS             complement(8573..8845)
                     /locus_tag="VGM46_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEY4192763.1"
                     /translation="MPATIDIYDLKGLNCPMPVLKAKKKLAGMRPGSLLWLETTDPLA
                     TLDIPAFCREDGHRLVETVADSGMHRFLVERGFQQKYETVCVRKCG"
     gene            complement(8853..10175)
                     /locus_tag="VGM46_08975"
     CDS             complement(8853..10175)
                     /locus_tag="VGM46_08975"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase family protein"
                     /protein_id="HEY4192764.1"
                     /translation="MFAKLARTGLLIAAIAVAGCTDSSMKDFSDVASKQLPDKILADM
                     KAKGMVRNSSVMARIFKEEGKLEIWKAKTNGRYDIVASYDICKWSGKLGPKFTEGDRQ
                     APEGFYTVRPSQMNPNSKYPLAFNIGYPNAYDRANGRSGANLMVHGACSSSGCYSMTD
                     AQIEQIYAFGRDAFQGGQTEFQIQAFPFRMTAANMARYRNDPNYGFWKMLKVGYDNFE
                     VTKVPPKVDVCEKRYVFNQIAADGQTFNPTGACPATTQPDSLKSAFNSYQSSYDAAYG
                     SAIKASTPAPRPTIAGLKEAALVSDWSKKRARGERVPIEPPSLNSDGSITETARMGRI
                     DSPAGRKMAALDAEKAAKEKAAAEKEAQIAAAKAAKEAAKAQAIAEKEAAKQQLADAK
                     AAEEAPAATGTVPAAQEAEAMPVAETQTAQSDGTVSKLKKKLLGMFGG"
     gene            complement(10391..11341)
                     /locus_tag="VGM46_08980"
     CDS             complement(10391..11341)
                     /locus_tag="VGM46_08980"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012652597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEY4192765.1"
                     /translation="MYNYLDFEKPVADLEGKIIELKKLAESGEAVDVADEIARLEKRS
                     RDALRDAYKALSPWQKVQVARHPDRPHCVDYISGLLTDFTPLAGDRNFGEDQAILCGF
                     ARFRGEPVAVIGQEKGSDTASRLKHNFGSVRPEGYRKAVRVMELADHFKIPLLTLVDT
                     AGAFPGVTAEERGQAEAIARSTSACLGLKVPSISVVIGEGGSGGAIAIATANRVYMLE
                     HSIYSVISPEGAASILWRDTTRSRDAATNMKITAQDLLEMKIIDAIVPEPLGGAHRAP
                     QAVIAATGDLIARTMKDFAGSNADFREQRREKYLAMGRSL"
     gene            complement(11439..12356)
                     /locus_tag="VGM46_08985"
     CDS             complement(11439..12356)
                     /locus_tag="VGM46_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEY4192766.1"
                     /translation="MNSAARIEAFLEMMSAERGAAENTLSSYRRDLEDASEQIAGGLA
                     TAGTGDLRAYLNDIAARGFAATSQARKLSAIRQFYKFLYAEGLRADDPTGTLDSPRKD
                     RPLPKTMSEADTGRLLDRAALEAGDPAQGEGDPLAALRLHALVEVLYATGLRVSELVG
                     LPVTVAQRDDRFFMVRGKGDKERMVPLSAKARTAMKTWLGARQKVPALAQSPFLFPAA
                     SESGALSRQVFARDLKGLAARAGIASAKISPHVLRHAFASHLLQNGADLRAVQQLLGH
                     ADISTTQIYTHVLEERLVRLVNDHHPLAD"
     gene            complement(12353..12520)
                     /locus_tag="VGM46_08990"
     CDS             complement(12353..12520)
                     /locus_tag="VGM46_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY4192767.1"
                     /translation="MPTLFRFVVTLLILAGIAYGVMFALVLFVEPKKAEISVRIPAAR
                     LDPAIKRYPAK"
     gene            12687..13325
                     /locus_tag="VGM46_08995"
     CDS             12687..13325
                     /locus_tag="VGM46_08995"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006200256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEY4192768.1"
                     /translation="MNAQPQPPQAGSHAELIERLGGCSIVFVGLMGAGKTAIGRKVAT
                     MLGLPFIDSDHEIESVSRMTVPELFEHYGESEFRALEQRVILRLLENGPQVLSTGGGA
                     FMNEQTREAIAGQGVSVWLKADLDLLMERVSKKQNRPLLKNPDPRGVLARLMDERYPV
                     YATAAIAVQTRDERKETIAAEVIEALERHYSIAGAVAGGVEAGGVEAGGVEE"
     gene            13322..14458
                     /gene="aroB"
                     /locus_tag="VGM46_09000"
     CDS             13322..14458
                     /gene="aroB"
                     /locus_tag="VGM46_09000"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEY4192769.1"
                     /translation="MNETVTVTVGLGERAYDILIGPGLLARAGEEIALRLSGTRAAIV
                     TDENVAAAHLDTLKAGLARGGVEAAVITLPPGEKTKSFEHLQDVVDGVLAARLERRDT
                     VIAFGGGVIGDLAGFAAGITRRGMNFVQIPTSLLAQVDSSVGGKTGINSARGKNLVGV
                     FHQPKLVLADTEVLDTLPIREFRAGYAELAKYGLIDRPDLFDWLEKNWQAVFAGGPAR
                     ITAIAEACRAKADVVARDEFEQGDRALLNLGHTFGHALEAATQYDGARLVHGEGVAIG
                     MALAHRFSSRMNLASPDDAARVEAHLKAVGLPWRMADIAGELPDAEALLGFIAQDKKV
                     SRGALTFILTRGVGQSFIARDVPASEVLSFLKENHPGDGGKGRA"
     gene            14455..15360
                     /locus_tag="VGM46_09005"
     CDS             14455..15360
                     /locus_tag="VGM46_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018429722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HEY4192770.1"
                     /translation="MNEAGWIAAGVLAVLCAAAFALRARLLAALGLELRAVCADEPRN
                     GASAERNGEPAGAARGRLDSLADLDELEVSDIMVHRTNMRSVNADNAPEAVVREILQS
                     PHTRLPLWKGSLDNIVGMLHAKDVLRALNEVDNDFSRVDVMKVASKPWFVPDTTTLRE
                     QLNEFLRRKAHFAIVVDEYGEVEGLVTLEDIIEEIVGEIADEHDIGMQGVKREADGSM
                     VVDGTVPIRDLNRALGWDLPDDEATTIAGLVIHEAQSIPEEKQAFTFHGKRFIVMKRD
                     KNRIARVRIKPVPVEAGPQIPPKRL"
     gene            complement(15383..15685)
                     /locus_tag="VGM46_09010"
     CDS             complement(15383..15685)
                     /locus_tag="VGM46_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HEY4192771.1"
                     /translation="MSIQATIENKLATAFSPQRLEIVNESHLHAGHHHVESGHHAQFD
                     GSGETHFRVRIVAGAFSGMSRLERHRAVNALLAEELKAGLHALAIETAAPGEATRW"
     gene            15828..16451
                     /locus_tag="VGM46_09015"
     CDS             15828..16451
                     /locus_tag="VGM46_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006200248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEY4192772.1"
                     /translation="MKPYPKYFEKIRVRPDKEAEAKSHAPICQWDGCKEAGTHRAPVG
                     RMKEGEYFRFCINHVREYNKSFNYFSGVPDSEVARFQKEAMTGNRPTWKMGVNGAATT
                     GSSPDYAKARSGRAGYHNRMRDPFDLFKGPKDPRQPRERKAKPLEAKALETLGLDTKA
                     TGNDIKARYKELVKRHHPDANGGDRGSEDRFRDVLQAYRVLKQAGLC"
     gene            16575..17561
                     /gene="cobS"
                     /locus_tag="VGM46_09020"
     CDS             16575..17561
                     /gene="cobS"
                     /locus_tag="VGM46_09020"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006328376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobS"
                     /protein_id="HEY4192773.1"
                     /translation="MNKVDRDIANLPDTTVSVKDHFGFDSKMVIPAYSVTSEHVPDLD
                     PDYLFDPQTTSAILAGFAYNRRVMVSGYHGTGKSTHIEQVAARLNWPMVRVNLDSHVS
                     RIDLVGKDAIVVKEGMQVTEFRDGILPWAYQHNIALCFDEYDAGRPDVMFVIQRVLEA
                     SGRLTLLDQSRVIRPHPAFRLFATANTVGLGDTTGLYHGTQQINQAQMDRWSIVTTLN
                     YLPHDNEVNIVVAKAKHYRDAKGRETVSKMVRVADMTRSAFVNGDLSTVMSPRTVITW
                     AENAEIFGDTGMAFRLTFLNKCDELERPVVAEFYQRAFGEDLPESAANVVLG"
     gene            17574..17921
                     /locus_tag="VGM46_09025"
     CDS             17574..17921
                     /locus_tag="VGM46_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY4192774.1"
                     /translation="MNYQGGCHCGKVGFSVELDLSSPITCNCSYCTQRGSILAFAPVE
                     NFSLEKGDGELTEYLFNTNKISHLFCASCGMESFARATAPDGIKMVAVNIRCLEGVDP
                     DAVTPHRFDGRSR"
     gene            17918..19828
                     /gene="cobT"
                     /locus_tag="VGM46_09030"
     CDS             17918..19828
                     /gene="cobT"
                     /locus_tag="VGM46_09030"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009365010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobT"
                     /protein_id="HEY4192775.1"
                     /translation="MTGRDMAGRGDNTRNKSKTGAEADGFKRAITVCMRAISGDKEME
                     IAFAKDKPALAGNRARLPELAKKPTRADIAVTRGLGDSMALKRACHDQRIHSKLAPEG
                     KQARAVYDAVEQARVEAIGSRMMAGVGDNIGAMLEDKYAKANLADVSDKADAPVEEAL
                     ALLVREKLTGRTVPRSAEKLVDLWRPWIEQKAGSGLDGLSDKLGDQQAFARLVRDMLA
                     SMEMAEELGDDQETEDAEDDENQPQGEEENQESGEDDSGSEDSQSEDAEASSDEEEQS
                     AESESSDATADDDSESEDADAETPGETKRNDNPFANLPKEIDYKVFTNAFDETVGAEE
                     LCDEEELDRLRAFLDKQLANLSGVVGRLANRLQRRLMAQQNRSWDFDLEEGYLDPARL
                     VRVVIDPMQPLSFKQERDTKFRDTVVTLVLDNSGSMRGRPITVAATCADILARTLERC
                     GVSVEILGFTTRAWKGGQAREKWLKDGKPPAPGRLNDLRHIIYKSADAPWRRARRNLG
                     LMMREGLLKENIDGEALLWAHQRLMARPEQRKILMMISDGAPVDDSTLSVNPGNYLER
                     HLRAVIDLIETRSPVELLAIGIGHDVTRYYRRAVTIVDAEELAGAMTEQLASLFEEEP
                     APGHRGGARRAR"
     gene            19999..20970
                     /locus_tag="VGM46_09035"
     CDS             19999..20970
                     /locus_tag="VGM46_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HEY4192776.1"
                     /translation="MLVFCAPAFAAGRGAAVPMEVSVRPIGEFQIGRAVKNFGPLEFV
                     GGLEMTASNGDFGGLSAFRFLKPGSDFIGVADTGFWFFGTIVHDADKRPSGVENFRMA
                     QMVDEAGQPIDKKWEVDAEGLAVRDGIATVGFERNHRIARFKIDAEAMEAPFGQLDFL
                     VPKKELRQNRGFETVTTSSPDGPDKGALVVVSEKSLDKAGNVYAAVLGGPRKGVFTVK
                     RDGAFDITDGAFLPNGDLLLLERSFSMASGVGMRLRRIDGGSIGNGALADGPVLMEAD
                     MGHQIDNMEGLDLWTRDDGALMVSLISDDNHSILERNLYLEFILHQD"
BASE COUNT         3928 a         6590 c         7090 g         3809 t
ORIGIN      
        1 gtttgccggc ctttgccagg cgttcgatgg cgcggtcgta atagccgccg ccatagccga
       61 tgcggtggcc gcgcgcgtcg aaggcggcga gcggcaccag catgatttgc gggtcgagca
      121 acttcgcctc ctcatgcggg ccgatcgtgc cgaagcccat ctcgaccatc ggcgcgccgc
      181 gcaccagttc gcgaaagacg atggtggtct tgtcgaggat ggccggcagg caaagccgcg
      241 cgccgttttc gagcagcgca aacatcagcg ggcgaacatc gacttccgag cgcatcggcc
      301 agaagccgga gacgacctgt cccggctcga cggcgatttt gtcgcgcgcc gttccggcca
      361 tgtcaagcgc cgcttcgacg cgccagaacg gatcgaggcc atcgcggcgg gcaagcgctt
      421 cgcggcgcaa ttgcttcttg atttcgctgg gagaagtcat cggaaggcga ggatgcaccg
      481 aaatcggctt ttgaagaagt gacgatccac gcgaccggtg gagatgggat cccgggaacc
      541 tacaaagtag gtgggcgccg tgtgtccaaa cccacgggcc tggacaggga cagctcccta
      601 gggatcatta aggccccggg gaaaatggtc tcctgccggg aagcacagat cgtcaccgcc
      661 aatataggcc gatgactgcc ggttagccag atgcggcgac gcccgcatct ggctttattc
      721 gctgtctgcc agcggcactt gcggggcagg acccggctcc gtagggtttg agcgacacaa
      781 ggacagaaaa gaatgcgctt cgaaggcacg gcggcctatg tcgccgacaa ggatctgatg
      841 gtggcggtca acgcggccat cgcgctggaa cggccgctgc tggtcaaagg cgaacccggc
      901 accggcaaga cggaactggc ccggcaggta gcggcggcgc tagggctgga actgatcgaa
      961 tggaacgtca agtcgaccac gcgggcgcag caggggcttt acgaatatga tgccgtgtca
     1021 cggctgcgcg acagccagct tggcgacgag cgcttcaacg acattgccaa ttacatcaag
     1081 cgcggcaaat tgtgggacgc cttcgcggcc ggccgcaaga tcgtgctttt gatcgacgag
     1141 atcgacaagg ccgatatcga atttccaaac gacctgttgc aggaactcga taagatggag
     1201 ttcttcgtct acgagacggg cgaaacgatc cgggcggcgg tgcgccccat cgtcatcatc
     1261 acgtccaaca acgaaaagga actgcccgac gccttcctgc gccgctgttt cttccactat
     1321 atccgctttc ccgatgtcga gacgctgcac agaatagtcg aggttcacta tcccggcatc
     1381 aagcagaacc tggtgcgcgc cgcacttacc cagttctacg aaatccgcga cgtgccgggg
     1441 ctgaagaaga agccatccac ttcggaagcg ctggactgga taaggctgct aatcgccgac
     1501 gacatcgatc cagccgatct gcgcgccgat gcgaaaaacg cgctgccgaa gctgcacggc
     1561 gcattgctga agaacgagca ggacgtgcat ttgttcgagc gcctggcttt catggcgcgg
     1621 cggcaagggt gaggaacggt cgcatagcgg acgaacgccc gcagcgatag tgaagggccg
     1681 ggcatatcga acatcaagga gcaagccaga tgacggatat tacggtccga tcgctcgaaa
     1741 agtccgacca tgccgattgg cggcggctgt ggacgagcta tctggatttt tacgagacga
     1801 aactgccgga agaaatatac gcgacgacct gggagcgcct tttcagcggc ggcgagttcg
     1861 agccgaaagg cttgattgcg ctggtcgacg gcaaggctgt cggcttgacg cattatcttt
     1921 accaccggtc cggctggtcg ctggccaata cctgctattt gcaggatttg tttgccgacg
     1981 agaccgcgcg cggcaagggc gtcggcgccg cgctgatcga ggcggtgcgg cgggaggcgg
     2041 aaaagctcgg cgtcgacaat gtctactggc tgacgaacga gaaaaacgcc acggcgcgca
     2101 agctctacga ccgcgtggcg cggcattccg ggttcatcaa gtatcgcctg tcgtagcgcg
     2161 gcgatgttca tcccgttctt cctcgaactg aaggccgcga aaattccggt ttcgctgcgg
     2221 gaatatctgt cgctgctgga ggggctggag gcggggctgg tcgattacga tgccgagggg
     2281 ttttactacc tcgcccgcgc cgcgctggtg aaggacgagc gccatatcga ccggttcgac
     2341 cgggtgtttg cgcatttctt caagggcgtc gaggcgctgg cgggggaagg cgccgtcgat
     2401 gttcccgatc tgcccgaaga ctggctgcgg aaactggcgg aaaagcatct cagcgacgag
     2461 gagaaaaagc tggtcgaggc gctgggcggc ttcgacagac tgatggagac gctgaagcag
     2521 cggcttgagg aacaaaagga gcggcatcag ggcggctcga aatggatcgg cacagccggc
     2581 acctcgccct tcggcgccta tggctacaac ccggaaggcg tgcgcatcgg ccagcaggaa
     2641 agccgcaacc ggcgcgccgt caaggtgtgg gacaagcgcg agttcaggaa tttcgacgat
     2701 ggcgtcgagc ttggcatccg caacatcaag gtcgcgctga agcgcctgcg ccgctgggtg
     2761 cgcgaagggg cggcggagga attcgacctg cccggcacca tccacgccac cgccgagcat
     2821 ggctatctcg acgtgcgcac ccgccccgag cggcgaaatg cggtgaagct tttgatgttc
     2881 ttcgatgtcg gcggctcgat ggacgagcat atcaaaagcg tcgaggaact tttctcggcc
     2941 gcgcgcgccg aattcaggca gctcgatttc ttctatttcc acaattgcgt ctacgagcat
     3001 gtctggaagg acaaccgccg ccgcgccgag accgtctcga ccttcgacct cgtccataaa
     3061 tacggagccg actacaaggc cgtcttcgtc ggcgacgccg cgatgagccc ttacgagatc
     3121 gcccagccgg gcggctcggt cgagcactgg aacaaggagg ccggcagcgt ctggctttcg
     3181 cgcctgctcc agcaatggcc gaatgcggtg tggctcaatc cggaagcgga aaagaactgg
     3241 ggctatacgc attccaccgg catgataggc gagcttttcg gcggccgcat gttcgggctg
     3301 acgctggccg ggctggaagc cgcgacccgg caattatcgc ggaagcactg aacgcttcag
     3361 gctttcggta cgattgccta agcctggttt ttcgcgcgca ggccgcgttc gcacagctcc
     3421 gactgaaccg catcctggag gctgagaatc tgggagcgga tcgtctcgtc cggcaggccg
     3481 gaggcgcgca tggaggcgac gatgccggca atgcgggtgc gccaaaatac gctggcggcg
     3541 gggccgtgca cgcattccag atcatcgacg atggagcgca cgagggcggc gcgggagggg
     3601 agcgggaagg gaatcagttc agccatgcag gcaaaatcaa cgatcgtggt ttatggaccg
     3661 taaacgccgg caaactgtct gccgaaacag cttcgtcggg cggtgcggcg agaagcatcg
     3721 gcgcttgata aaaaagcccc gcacgccagg catgcggggc tttgatctgg tgatcccgac
     3781 tggattcgaa ccagtggcct acagattagg aatctgccgc tctatccgac tgagctacgg
     3841 gaccatgcgg ccagacacat agcggcttcc agacgctgcg ccaagtgcga agctgcgttc
     3901 ggcgtggcgg cgctttgcgg gtctatgccg cgagcgcggt ttcggcgatc ttcacccagt
     3961 agctgatgcc gtgcggaatg gcctcgtcgt tgaagtcgta ggcggggtgg tgaaggccgg
     4021 cggtatcgcc attgccgatg aagatgaagg cgccggggcg agcctccagc atgtaggaga
     4081 aatcctcgcc gcccatcacc ggcggcatgg cgcggatgac cttggcgtca cccgccacgg
     4141 ctgccgccac gtcgccggcg aagacggttt cctcggcatg gttgaaggtg acggggtaat
     4201 tggcgtcgta gtcgatctcg atctcggcgt tgaacgttgt ggcgatgccg gcgcagatgg
     4261 cgcgcatgcg ctgctcggcc atcgccgcca tttccttttt cagcgtgcga accgtgccgg
     4321 cgatctctgc cttttcggga atgacattat aggcgtcgcc ggcgtggaat ttcgtcaccg
     4381 acaccaccag cgcctcgacc ggatcggcgt tgcgcgaggc gatcgtctgc aacgccgtca
     4441 ccagatggct ggtgatgacg atcgggtcga tggtgccgtt gggcatcgcc gcatggccgc
     4501 cgcgcccctt gacggtgatg gtgaattcgg cggtcgccgc catgatcggg ccggggcgga
     4561 tggcgaagca gccgaccggt agggtcggca tgttgtgcat gccgaaaacc ttcgaaatgc
     4621 cgaagcggtc catcatgccg tccttgacca tctcgttgcc gccgccgccg ccttcttcgg
     4681 cgggctggaa gatgacggcg accgagccgg cgaagttacg cgtttcggcc agatatttgg
     4741 cgccgccgag cagcatggcg gtgtggccgt catggccgca ggcatgcatc ttgtttggaa
     4801 ccgtcgaggc atagagcttg ccggtgatct cgttgatcgg cagggcatcc atgtcggcgc
     4861 gcaggccgat ggtttcgccc gcgccgagcc ggccgcggat gatgccgacg acgccggtgc
     4921 ggccaaggcc ggtcaccact tcgtcacagc cgaattcctt cagcttctcg gtgacgaagg
     4981 cggcggtctg gaagacatcg aaattaagct ccggcgtctg gtgcaggtgg cgtcgccagc
     5041 cgctgacttc ttcctgcatc tcggcggcgc ggttcaggat gggcatggtg gcatctcgct
     5101 gttgatttgc ttttgtcggg cttgctgcaa tcgtgatcgc ccggtgcttt gccatgttag
     5161 tgtcacatag gctaaatagc accggagaaa atcgcgggcc gatgtccgct acaccacggt
     5221 gccgcctggc tgttgaaacc ttacggagac aaccgcgatg acggcaagag gcgatttcgg
     5281 atggcatcat gaattccagg cgttttccaa gacttgtact ggccggcatg gttgccctgc
     5341 cggcgttctg cgcgccggtg atggccaacc cttcgctgct gttcgacctg aaaaccggca
     5401 aggtgctgga gcatcgcgat gccttcaagc gctggtatcc ggcttcgctg tcgaagctga
     5461 tgaccgccta tgtgaccttt cgcgccatcg atgccggcga tgtccggctt gattcgccga
     5521 tcaaggtgac caagcattcg gccgccgagc cgccgtcgaa aatgggcttc aaaccgggcc
     5581 aggtgatgcg gctggacaat gcgatgaaga tgatgctggt caagtcggcc aacgacatcg
     5641 ccatggccat cggcgagaat gtcggcggtt cgcaggcggc gtttgccgag cgcatgaatg
     5701 ccgaagcgca aaggctcggc atgaccggct cgcatttcgt caacccgaac gggctgtttt
     5761 cgaccgagca atatacgacg gcgcgcgacc ttggcctgct ggtgatggcg atccgcaacg
     5821 aatatccgca atatgcctcc tggttctcca tcgaggggct ggcgctcggc aagcgcacca
     5881 ttccgaacta caatcttctg gtcggccgct atcccggcgc tgacggcatg aaaacgggct
     5941 tcgtctgcga atcgggcttc aaccagatcg gctcggcgac gcgtaacggg cgcacgctgg
     6001 tggccgtggt gctgggcgaa cagacggcga tcacccgcgc cgaggtggcg gcaaaactgc
     6061 tcgaccaggg atttgccatc ccgacgagcg ccgcgaccac cgtggccgcc ctgccaagct
     6121 atggcgacgc cgtatctgaa aacaacatgc gcgaagagct gtgcagcaag aagccggcga
     6181 gcgcgcagtc cgaagcgccg gtcgtcgccg ccaagccgaa atcgccctac caggtcaagc
     6241 tggttgcgcc tacgctggtg gcggtcagcc tcggcggcgc gacaggcccg ctgcccaagg
     6301 cgatggtcgg caaggtcgaa tatgcggacg tgccgacgcc gagctggcgg ccggaccggc
     6361 catcgccgtt cggcgaggat gccgtggccg cccagggcga ccagtcggcc aagggccttt
     6421 gagcgtgcag ggcttgccga tcccggtttc ggtgctgacg gggtttctgg gcgccggcaa
     6481 gacgacgctg ctcaaccggc tgctgaaaga cccggcgctc accgataccg ccgtcatcat
     6541 caacgagttc ggcgacgtgg ccatcgacca cctgctggtc gagcaggcat cggacggggt
     6601 catccagctt tccgacggtt gcctgtgctg caccgtgcgc ggcgaacttg tcgatacgct
     6661 ggccgatctg gtcgaccggc tacagacggg ccgcatcaag cggcttgccc gcgtcatcat
     6721 cgaaacgacc ggccttgccg accccgcgcc ggtgttgcag gcgatcatgg cgcatccggt
     6781 tctcgtccag gcattccggc tcgacggcgt catcaccatc gtcgatgcgg tcaatggcag
     6841 cgcctcgctc gatggccatg tcgaggcggt gaagcaggcg gcggtggcgg accgcatcgt
     6901 tttgaccaag accgatctgg cggacgacat tcgcgccgtc gatgcgttgc gccttcgcct
     6961 gcgcggcctc aacccgaatg cggcgctgct cgacgccggc gatccgggca cgggcgtggc
     7021 gtcgctgttc gattgcgggc tttacaaccc ggccaccaaa accgccgacg tgcgccgctg
     7081 gctgggcgag gaagcgcagc atgaccacag cccgcacggt catgaggggc ctgatcatcg
     7141 ggggcatgaa ggccacgacc acggccatcg ccacgatgcg agggtgaaat cgcatacgct
     7201 catccatgac gggccggtgc cgcttgcggc catcgagatg tttctcgacc tgctgcgctc
     7261 gacgcatggc gaccatctgc tgcgcatgaa gggcatcatc gaactgagcg acgaccgctc
     7321 gcggccgctg gtcatccatg gcgtgcagaa gacgctgcat ccgccaatcc ggctggcggc
     7381 gtggccggac gaacaacgcg gcacccggct ggtgctgatc acgctcgaca tgccgcagga
     7441 ttatgtgcag cggctgtttg cggctttcac caacaccccc gccatcgata cgccggaccg
     7501 ggcggcgctg gaggaaaacc cgctggcgat tgccgggcgt cggtagcaag ggcggtaaaa
     7561 gcgatgaaaa agaccgaagc gagcgaaggc cggtctcctt cccaacagat cgatgcaagg
     7621 atcgccgagc tgggtgactg gcgcggcagg acgctggccc atatccgcgc gctgatcatg
     7681 caggctgacc ccgaggcggt cgaggagtgg aagtggcgcg gcgtacctgt ctgggagcat
     7741 ggcgggcaaa tctgcaccgg cgagacctac aagagcgccg tcaagctgac cttcctgaag
     7801 ggggcatcgc tgcccgaccc atccggtctt ttcaacgcca gccttgaagg caatgcgcgc
     7861 cgcgccatcg acctgcatga gggcgatgaa atcgacgagg acgccttcaa gacgctcatc
     7921 tgcgccgccg tcgcgctgaa catggcgaag gcacggaaat aagccggcct atcccgttta
     7981 acagcgcccg ctcagccggt attccagcca taataggtca gcgggtcgcc gctgcgcgga
     8041 tgcacgccat ggcgcagcac ggtcaggccg gccttctcat agaagcggca ggctctttcg
     8101 ttggttgcgc gcgtatggag cgtgaagccg tccggcatga cggccattgc gtgtcggagc
     8161 agcgcgcggc caataccggc gcccagcgat ccgggccgca cgaacagctc ggccagaacc
     8221 gcttccgctg gcctgatggc gagaaagccg acgatctcgt cgccgcgaca ggcaagcgtg
     8281 acctgccagc cggcggcaaa ttccgcgtcg agccggcctc gcaactcggc ttcggtcggc
     8341 ataacggcgg gtccgacgcc gggcaggctg gcgctgcggt gccagatttc ggcaatcgca
     8401 tcgtaatccg cctcgcatga cgggcgcagt tcgcaggctt gccttgcatc attcatgacc
     8461 ggacccaatc attatgacca gacgcatggc gctgaccgca aaggcaaggg cgtcgcatcc
     8521 tggagctgtt ctggttttca ccgaatcgac aaaaccgctc catctctttg ttttagccgc
     8581 atttccggac gcaaaccgtt tcatactttt gctggaatcc gcgctcgacc agaaagcggt
     8641 gcatgccgct gtcggccacc gtctccacca gccggtggcc gtcctcgcgg caaaaggccg
     8701 gaatgtcgag cgtggccagc ggatcggtgg tttccagcca cagcaggctg ccgggccgca
     8761 tgcctgccag cttctttttg gctttcagca ccggcatggg gcagttcagc cccttgaggt
     8821 cgtagatgtc gatggtggcg ggcaaggcgg cgtcagccac caaacatgcc gagcagcttt
     8881 ttcttgagct tggagacggt gccgtccgac tgcgcggtct gcgtctcggc aaccggcatg
     8941 gcctcggctt cctgcgcggc tggaaccgtg ccggtggcgg cgggggcttc ctcggccgcc
     9001 ttggcgtccg ccagttgctg cttggcggct tccttttcgg cgatggcctg cgccttcgct
     9061 gcttcctttg ccgccttggc ggcggcaatc tgggcttctt tctcggcggc ggccttctcc
     9121 ttggcggcct tttcggcgtc gagcgccgcc atcttgcgcc cggccggcga atcgatgcgg
     9181 cccatgcgcg ccgtttcggt tatcgagcca tccgagttga gcgacggcgg ctcgatcggc
     9241 acgcgctcgc cacgggcgcg cttcttcgac cagtcggaaa caagtgccgc ttccttgagg
     9301 ccggcgatgg tcggcctggg cgcgggcgtg ctggccttga tggcgctgcc ataagcggca
     9361 tcgtaggagc tttgatagga attaaaggca cttttcagcg aatccggctg ggtggtcgcg
     9421 gggcatgcgc cggttgggtt gaaggtctgg ccatcggcgg cgatctggtt gaagacgtag
     9481 cgcttctcgc agacatcgac cttcggcggc accttggtga cctcgaaatt gtcgtagccg
     9541 accttcagca tcttccagaa gccgtagttc gggtcgttgc ggtagcgcgc catgttggcg
     9601 gcggtcatgc ggaacggaaa agcctggatc tggaattcgg tctgcccgcc ctggaaagcg
     9661 tcgcggccaa aggcgtaaat ctgctcgatc tgggcgtcgg tcatcgaata gcagcccgac
     9721 gacgagcaag cgccatgcac catcagattg gcgccggagc ggccattggc gcggtcatag
     9781 gcgttcggat agccgatatt gaaggcgagg gggtatttcg agttcgggtt catctgcgag
     9841 gggcgaacgg tatagaagcc ttccggcgcc tggcggtcgc cctcggtgaa tttcgggccg
     9901 agcttgcccg accatttgca gatgtcgtag ctggcgacga tgtcgtagcg gccgttggtc
     9961 ttggccttcc agatctccag cttgccttct tccttgaaga tgcgcgccat gacggatgaa
    10021 ttgcgcacca tgcccttggc cttcatgtcg gccaggattt tgtcgggcaa ctgcttgctg
    10081 gcgacgtcgg aaaagtcctt catggacgaa tccgtgcagc ccgcaacggc gattgcggcg
    10141 atcaggagtc cggtgcgggc aagctttgca aacatggatc ggctatcgtc ttcattgtgg
    10201 cgcggcattc ccgcgccttc tttcggtcgt ctcgaatgga ccttaagtcc gttaaccttg
    10261 aaaattcctt accgcaagcg cgccccaagc ccgttgccgc gcctccactg acctgattgc
    10321 agcggcattt ttgtggcaaa gatggggcgt ccgccgcaat tggccggcag cggcggcaag
    10381 ttcgccgcgt ctagaggctg cggcccatgg ccaggtattt ttcgcggcgc tgttcgcgga
    10441 aatcggcgtt cgagccggca aaatccttca tcgtccgggc aataaggtcg ccggtcgcgg
    10501 cgatcaccgc ctgcggcgca cggtgcgcgc cacccagcgg ctccggcacg atggcgtcga
    10561 tgatcttcat ttccagcaaa tcctgcgcgg tgatcttcat gttggtcgcc gcatccctgg
    10621 agcgcgtggt gtcgcgccac aggatcgagg cggcaccttc cggcgagatc accgaataga
    10681 tcgaatgttc cagcatgtag acgcggttgg cggtggcgat ggcgatggcg ccgccggaac
    10741 cgccttcgcc gatgaccacc gagatggacg gcactttcag gccaaggcag gccgaggtcg
    10801 agcgggcgat ggcttcggcc tggccgcgtt cctcggcggt gacgccggga aaggcgccgg
    10861 cggtgtcgac cagcgtcagc agcggtatct tgaaatggtc ggccagttcc atcacacgca
    10921 ccgccttgcg gtagccttcg gggcgcaccg agccgaaatt gtgcttgagg cggcttgccg
    10981 tgtcggagcc tttttcctgg ccgatcaccg ccacaggctc gccgcgaaag cgggcgaagc
    11041 cgcacaggat cgcctggtcc tcgccgaaat tgcggtcgcc agccagcggc gtgaaatcgg
    11101 tcagcaggcc gctgatatag tcgacgcagt gcggccggtc ggggtggcgg gcgacctgca
    11161 ccttctgcca cgggctcagc gccttgtagg cgtcgcgcag cgcatcgcgc gagcgctttt
    11221 ccagccgggc gatctcgtcg gcgacatcga ccgcctcgcc gctttcggca agcttcttca
    11281 gctcgatgat cttgccttcg aggtcggcga ccggtttttc gaaatcaagg taattataca
    11341 tgcttgagcg gaccgatacg ccaaagattg agccaagcga aaccgaaaag ccacggccgc
    11401 gggcagcgga atgtcgcctt cacatagcgg tagagagcct agtcggcaag cgggtgatga
    11461 tcgttgacca gccgcaccag ccgctcttcc agcacatggg tgtagatttg cgtggtcgat
    11521 atatcggcat ggccgagcag ttgctgcacg gcgcgcaggt cggcgccatt ttgcagaaga
    11581 tggctggcaa aggcgtggcg cagcacatgc ggcgatattt ttgccgaggc gatgccggcg
    11641 cgcgcggcaa gccctttcag gtcgcgggca aacacctgcc gcgaaagcgc gccgctctcg
    11701 gaagccgccg gaaacaggaa cgggctttgc gccagcgccg gcactttctg gcgcgcaccg
    11761 agccaggtct tcatcgccgt cctcgccttg gccgacagcg gcaccatgcg ttccttgtcg
    11821 cccttgccgc gcaccatgaa gaagcggtcg tcgcgctgcg ccaccgtcac cggcaggccg
    11881 accagttcgg aaacgcgcaa gccggtggcg tagagcactt cgaccagcgc atggaggcgc
    11941 agcgccgcca gcggatcgcc ttcgccctgc gccgggtcgc ctgcttccag tgcggcgcgg
    12001 tcgagcaggc ggccggtgtc ggcctcgctc atcgtcttgg gcagcgggcg gtccttgcgc
    12061 ggtgagtcca gcgtgccggt cgggtcgtcg gcgcgcaggc cttcggcgta gaggaacttg
    12121 tagaattggc gaatggcgga aagttttcgc gcctgcgagg tggcggcaaa gccgcgcgcg
    12181 gcgatgtcgt tcagataggc gcgcaggtcg ccggtgccgg ccgtggccag gccgcccgct
    12241 atctgctccg aggcgtcttc gaggtcgcgg cgatagctgg aaagcgtgtt ttcggcggca
    12301 ccgcgttcgg cgctcatcat ttccagaaag gcctcgatgc gggcggcgct gttcatttgg
    12361 cgggatagcg ttttatggcg gggtcgagcc tggcggctgg aatgcgcacg ctgatctcgg
    12421 cctttttcgg ttcgacgaac agcaccagcg caaacatcac gccataggcg atgccggcca
    12481 ggatgagcaa cgtcacgaca aagcgaaaca gcgtcggcat tctctttcgc ccgtctattt
    12541 tccgaataac taatatcccc gtcccgttat gggacgccgc aaaatccatt tcaagccgca
    12601 agcggcacgt agccgggttc gccaagccat gcttgacgta aacggccttt tcatgtcgat
    12661 acggagcctc gatggcccgg aattcgatga acgctcaacc gcaacccccg caagctggaa
    12721 gtcatgccga gctgatcgag cggctgggtg gctgctccat cgtgttcgtc gggctgatgg
    12781 gcgcgggcaa gacggcgatc gggcgcaagg tcgccaccat gctggggctg cccttcatcg
    12841 acagcgacca cgagatcgag agcgtttcgc gcatgacggt cccggaactg ttcgagcatt
    12901 atggcgagag cgagtttcgc gcgctggagc agcgcgtcat cctgcgcctg ctggaaaacg
    12961 gcccgcaggt tctgtcgaca ggcggcggcg ccttcatgaa tgagcagacg cgcgaggcca
    13021 ttgccgggca gggcgtttcc gtctggctca aggccgatct cgatcttctg atggagcgtg
    13081 tttccaagaa gcagaaccgg ccgcttttga aaaatcccga tccgcgcggc gtgctggcgc
    13141 ggctgatgga cgagcgctat ccggtctatg ccaccgccgc catcgcggtg cagacccgcg
    13201 acgagcgcaa ggaaaccata gcggcggaag tgattgaggc gctggagcgg cattattcaa
    13261 ttgccggcgc cgttgccggc ggggtggagg ccggcggggt ggaagccggt ggggtggaag
    13321 aatgaacgaa acagtcaccg tcaccgtcgg tcttggcgag cgcgcctatg acatattgat
    13381 cggccccggc ctgctggcgc gcgccggcga ggagatcgcg ctgcgccttt ccggcacgcg
    13441 cgcggcaatc gtcaccgacg agaacgtcgc cgccgcgcat ctcgatacgc tgaaggcggg
    13501 gttggccagg ggcggcgtcg aggcggccgt catcacgctg ccgcccggcg aaaagaccaa
    13561 gagcttcgag catttgcagg atgtggtgga cggcgtgctg gccgccaggc tggagcgccg
    13621 cgataccgtc atcgccttcg gcggcggcgt catcggcgac ctggccggct ttgccgccgg
    13681 catcacgcgg cgcggcatga acttcgtgca gataccgaca tcgctgctgg cgcaggtgga
    13741 ttcctccgtc ggcggcaaga ccggcatcaa cagcgcgcgc ggcaagaacc tcgtcggcgt
    13801 cttccatcag ccgaagctgg tgctggccga tacggaagtg ctggatacgc tgccgatccg
    13861 tgaattccgt gccggctatg ccgagcttgc caaatacggg ctgatcgacc ggcccgacct
    13921 gttcgactgg ctggagaaga actggcaggc ggtgtttgcc ggcgggccgg cgcgcatcac
    13981 ggcgatcgcc gaggcctgcc gcgccaaggc ggatgtcgtt gcccgcgacg agttcgagca
    14041 gggcgaccgc gcgctgctca atcttggcca tacgttcggc cacgcgctgg aggcggcgac
    14101 gcagtatgac ggcgcgcgtc tcgtccatgg cgagggcgtt gccatcggca tggcgctggc
    14161 gcaccgcttt tcctcgcgca tgaaccttgc cagccccgac gacgcggcgc gtgtcgaggc
    14221 gcatctcaag gctgtcggcc tgccatggcg aatggccgat attgcaggcg aactgccgga
    14281 cgccgaggcg ctgctcggct tcatcgcgca ggacaagaaa gtgtcgcgcg gcgcgctcac
    14341 cttcattctg acgcgcggcg tcggccagtc cttcatcgcc agggacgtgc ctgcctccga
    14401 ggtgctttcg ttcctgaagg aaaaccatcc cggcgatggc ggaaaaggtc gggcatgaac
    14461 gaagcgggct ggatcgccgc cggcgtcctg gctgtgcttt gcgccgcggc gttcgccctg
    14521 cgcgcccgcc tgcttgccgc gcttggcctt gaattgaggg cggtgtgcgc cgacgagcca
    14581 cgcaacggcg catcggccga gcgcaatggc gaaccggccg gcgcggcccg cggccggctg
    14641 gacagcctgg ccgacctcga cgaactggaa gtctcggaca ttatggtcca ccgcactaat
    14701 atgcgctcgg tcaatgccga taacgcaccg gaagctgtgg tgcgcgaaat cctgcaaagc
    14761 ccgcataccc ggctgccttt gtggaaaggc tcgctcgaca acatcgtcgg catgctgcac
    14821 gccaaggacg tgttgcgggc gctgaacgag gtcgacaacg atttttcgcg cgtcgatgtg
    14881 atgaaggtgg cgtcgaaacc ctggttcgtg cccgacacga cgacgcttcg cgagcagctc
    14941 aacgagttcc tgcgccgaaa ggcgcatttc gccatcgtgg tcgacgaata tggcgaggtc
    15001 gaagggctgg tgacgctgga agacattatc gaggagatcg tcggcgagat cgccgacgag
    15061 cacgatatcg gcatgcaggg cgtcaagcgc gaggccgacg gctcgatggt ggtggacggc
    15121 acggtgccga tccgcgacct caaccgcgcg ctcggctggg acctgccaga cgacgaggcg
    15181 acgacgatcg ccggcctcgt catccacgag gcgcagtcga tccccgagga aaagcaggcg
    15241 ttcaccttcc acggcaaacg cttcatcgtc atgaagcgcg acaagaaccg catcgcgcgg
    15301 gtgcggatca agccggtgcc agtggaagcc ggtcctcaga ttccgccaaa gcggttgtag
    15361 gccgcctggc gggcgcagaa cctcaccagc gcgtggcctc gccgggggcg gcggtttcga
    15421 tggccagggc gtgcaggccg gccttcagct cttcggccag cagggcgttg acggcgcggt
    15481 ggcgttcgag gcggctcatg cccgaaaagg cgccggccac gatgcggacg cggaaatggg
    15541 tttcaccgct gccgtcgaac tgcgcatggt ggccgctttc gacgtggtga tggccagcgt
    15601 ggaggtggct ttcattgacg atttccagcc tctgcggcga aaaggccgtc gccagcttgt
    15661 tttcaatcgt cgcttgtatg gacattgcca cttcccttcg ccacatatgc cgcgccgcga
    15721 caaattcggc ctgccgcccg cataatgcct tgattcagcg gttagggcga tcaatcccga
    15781 aatgtcaatt cttgtttcgc agcgcgaaca gatcatagat acacccgatg aagccgtacc
    15841 cgaaatattt cgagaagatt cgcgtccgcc ccgacaagga agcggaagcg aagtcgcacg
    15901 cgccgatctg ccagtgggat ggctgcaagg aagccggaac gcatcgcgcg ccggtcggcc
    15961 gcatgaagga aggcgaatat ttccgcttct gcatcaacca tgtgcgcgag tataataaga
    16021 gcttcaatta tttctccggc gtgcccgaca gcgaggtcgc gcgcttccag aaggaagcga
    16081 tgacgggcaa ccgtccgacc tggaaaatgg gcgtcaacgg cgcggcgacg acgggatcgt
    16141 cgccggatta cgcaaaggcg cgctccggcc gcgccggcta ccacaaccgc atgcgcgacc
    16201 cgttcgacct gttcaagggg ccgaaagacc cgcgccagcc acgcgagcgc aaggccaagc
    16261 cgcttgaggc caaggcgctg gaaacgcttg gccttgatac gaaggcgact ggcaacgaca
    16321 tcaaggcgcg ctataaggaa ctggtgaagc gccatcatcc ggatgcgaat ggtggcgaca
    16381 gaggttcgga agaccggttc cgcgatgtgc ttcaggccta tcgcgtgctc aagcaggcgg
    16441 gcctgtgctg agcgagcctg cggtccatgc ttttttccaa ctttcatccg gttggaaaag
    16501 gttctaagac gcccccgaac atttcgattg ccggcgaagc gcaacggctt cgcccctgga
    16561 gacgttgagg cgttatgaac aaggtcgacc gcgacatcgc taacctgccg gacaccaccg
    16621 tttcggtgaa agaccatttc ggtttcgact ccaagatggt cattccggcc tattcggtga
    16681 cgagcgagca tgtgccggac ctcgatcccg actatttgtt cgacccgcag acgacctcgg
    16741 ccatcctcgc cggctttgcc tataaccgcc gcgtcatggt gtcgggctat cacggcaccg
    16801 gcaagtcgac gcatatcgag caggtcgccg cccgcctcaa ctggcctatg gtgcgcgtca
    16861 acctcgacag ccatgtcagc cgcatcgacc tcgtcggcaa agacgcgatc gtcgtcaagg
    16921 aaggcatgca ggtcaccgaa ttccgcgacg gcatcttgcc ctgggcctat cagcacaata
    16981 tcgcgctgtg cttcgacgag tacgatgccg gccgtcccga cgtcatgttc gtcatccagc
    17041 gcgtgctgga agcctcgggc cgcctgacgc tgctcgacca gagccgcgtc atccgtccgc
    17101 acccggcctt ccgcctgttc gccacggcga atacggtcgg cctcggcgac accaccggcc
    17161 tctaccatgg cacgcagcag atcaaccagg cgcagatgga ccgctggtcg atcgtcacca
    17221 cgctgaacta cctgccgcac gacaatgaag tgaacatcgt cgtcgccaag gccaagcatt
    17281 accgcgacgc caagggccgc gagaccgtca gcaagatggt gcgcgtcgcc gacatgacgc
    17341 gctcggcctt cgtcaatggc gatctgtcga cggtaatgag cccgcgcacg gtcatcacct
    17401 gggccgaaaa cgctgaaatc ttcggcgata ccggcatggc gttccggctt accttcctca
    17461 acaagtgcga cgaactggaa cgcccggtag tggccgaatt ctaccagcgc gccttcggcg
    17521 aggatttgcc cgagagcgcc gccaacgtcg tgctgggctg accggagcct gagatgaatt
    17581 atcagggcgg atgccattgc gggaaggtag gcttcagcgt cgagctggac ctgtccagcc
    17641 ccattacgtg caactgttcc tactgcaccc aacgcggcag catcctggct ttcgcgccgg
    17701 tcgagaactt ctcgctggaa aagggcgacg gcgagctgac cgagtacctg ttcaacacca
    17761 ataagatatc gcaccttttc tgcgcatcct gcggcatgga atcctttgcg cgcgccaccg
    17821 cgcccgatgg catcaagatg gtcgcagtca atatccgctg ccttgaggga gtcgatcccg
    17881 acgccgtcac gccgcaccgg ttcgacgggc gctcgcgctg acaggcaggg atatggcagg
    17941 ccggggcgac aatacacgca acaagtcgaa gacgggggct gaagccgatg gcttcaagcg
    18001 cgccataacc gtttgcatgc gcgccatttc cggcgacaag gaaatggaaa tcgccttcgc
    18061 caaggacaag ccggcgctgg cgggcaaccg cgcaaggctg cctgaactgg ccaagaagcc
    18121 cacccgcgcc gacatcgccg tcacgcgcgg ccttggcgat tcgatggcgc tgaagcgcgc
    18181 ctgccacgat cagcgcatcc acagcaagct ggcgcccgaa ggcaagcagg cccgcgccgt
    18241 ctatgatgcg gtcgagcagg cccgcgtcga ggccatcggc tcgcggatga tggctggcgt
    18301 cggcgacaat atcggcgcca tgctggaaga caaatacgcc aaggccaatc tcgccgatgt
    18361 ctccgacaag gccgacgcgc cggtcgagga agcgctggcg ctgctggtgc gcgaaaagct
    18421 gaccggccgc accgtgccca gaagcgccga gaagctggtc gatctatggc ggccatggat
    18481 cgagcagaag gccggttcgg gccttgacgg cctgtccgac aagcttggcg accagcaggc
    18541 cttcgcacgg ctggtgcgcg acatgctggc ctccatggaa atggccgagg aacttggcga
    18601 cgaccaggag acggaagacg ccgaggacga cgagaaccag ccgcagggcg aggaagagaa
    18661 ccaggaaagc ggcgaggacg attccggttc ggaagactcg cagtccgagg atgccgaggc
    18721 atccagcgac gaggaagaac agtcggccga atcggaatcg tccgatgcta ccgccgacga
    18781 cgattccgaa tccgaggatg ccgatgcgga gacgccgggc gaaaccaagc gcaacgacaa
    18841 tcccttcgcc aacctgccga aggagatcga ctacaaggtc ttcaccaacg ccttcgacga
    18901 gacggtgggc gccgaggagc tttgcgacga agaggaactg gaccggctgc gcgccttcct
    18961 cgacaagcag ctcgccaatc tttccggcgt cgtcggacgc ctcgccaacc ggctacagcg
    19021 ccggttgatg gcgcagcaga accgttcgtg ggatttcgat ctcgaagaag gctatctcga
    19081 tccggcgcgg ctggtgcgcg tggtcatcga cccgatgcag ccgctgtcct tcaagcagga
    19141 acgcgacacc aagttccgcg atacggtggt gacgctggtg ctggacaatt ccggttccat
    19201 gcgcggccgt ccgatcaccg ttgccgccac ttgcgccgac attctggcgc gcacgctgga
    19261 gcgctgcggc gtttcggtcg aaatcctcgg cttcaccacg cgtgcgtgga agggcgggca
    19321 ggcgcgcgag aaatggttga aggacggcaa gccgccggcg cccggccgtc tcaacgatct
    19381 gcgccacatc atctacaagt ccgccgacgc gccgtggcgg cgggcgcggc ggaacctcgg
    19441 cctgatgatg cgcgaggggc tgctgaagga aaacatcgac ggcgaggcgc tgttgtgggc
    19501 gcatcagcgg ctgatggcgc ggcctgagca gcgcaagatc ctgatgatga tctcggatgg
    19561 cgcgccggtc gacgattcga cgctgtcggt caatccgggc aattatctgg agcgccattt
    19621 gcgcgccgtc atcgacctga tcgagacgcg ctcgccggtg gagcttctgg ccatcggcat
    19681 cggccacgac gtcacccgct actatcgccg cgccgtcacc atcgtcgatg ccgaggaact
    19741 ggccggtgcg atgaccgagc aactggcctc gctgttcgag gaagagcccg cgcctggcca
    19801 tcgcggcggc gcgcggcggg cgcgatgagc ggccggccgc ttttacggct gcggcgttgt
    19861 tttctgaaat ttcctgaaat cgcgcgttta tggtcgcagt tcttcgaagt taggctgcgt
    19921 cctaccgccc atgcggggga gatggccggc aggccgagga ggggcaacgg gggacgccgg
    19981 tttgtcaccg tggccgccat gctggttttc tgcgcacccg ccttcgccgc cgggcgcgga
    20041 gcggccgtgc cgatggaggt ttccgtcagg ccgatcggcg aattccagat cgggcgcgcg
    20101 gtgaagaatt tcgggccgct ggaattcgtc ggcgggctgg aaatgactgc ttcgaacggc
    20161 gatttcggcg gcctgtcggc gttccggttc ctgaagcccg gcagcgattt catcggcgtg
    20221 gccgataccg gcttctggtt tttcggcacc atcgtccacg atgccgacaa gcggccatcg
    20281 ggcgtcgaga atttccgcat ggcgcagatg gtggacgagg ccggccagcc gatcgacaag
    20341 aagtgggaag tggatgccga gggcctggcg gtgcgcgacg gcatcgccac ggtcggcttc
    20401 gagcgcaacc accgcatcgc ccggttcaag atcgatgccg aggcgatgga agcgccgttc
    20461 gggcaactgg atttcctggt gccgaagaag gagctgcgcc agaatcgcgg cttcgagacg
    20521 gtcaccactt ccagcccgga cgggccggac aagggcgcgt tggtcgtcgt ttccgaaaag
    20581 agcctggaca aggccggcaa tgtctatgcc gctgtgctcg gtggtccgcg caagggggtc
    20641 ttcacggtca agcgcgatgg cgcgttcgac attaccgacg gtgcttttct gccgaacggc
    20701 gacctgcttt tactggaacg cagcttctcc atggcctccg gcgtcggcat gcggctgcgg
    20761 cgcattgatg gcggcagcat cggcaacggc gcactggccg atgggccggt gttgatggaa
    20821 gccgacatgg gccaccagat cgacaatatg gaaggcctgg atttgtggac gcgcgacgac
    20881 ggcgcgctga tggtgtcgct gatttccgac gacaaccatt cgatcctcga gcgtaatctc
    20941 tacctggaat tcatcctgca tcaggattga gggtgcaggc cgaagtggct gccgcacagg
    21001 tgccttcaac agctgttgcc ggcggcaggt tttcggtggc atccagcttc cctttcaggg
    21061 tatggcgcag gtttccccac cttcgtcatc ccggccgctg ccgagcagcc gaagggtgcg
    21121 aggcgagagc cgggatccat gccgtgatgc ctccggatgg atcgcagcgg cttcggcagc
    21181 taatcgtcgc cgtcacggca tggatcccgg acagccgcgt gccgctgcgc gccacacggc
    21241 ttccgggatg acgagagagg ggttgcggct cgcgcgctga gccgaggcgg cgcgggggac
    21301 agaagcgcgc aaaaaataaa tctatccaca ccctccgcgc ctgccggcat aatctcgcca
    21361 tctccctcgc gggaacacgg gtcatgaacc ggatgagcgt ggaggggacg gtgccgg
//
LOCUS       DASYCS010000285         2650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2972654,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000285 DASYCS010000000
VERSION     DASYCS010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2972654"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VGM46_09040"
     CDS             complement(<1..197)
                     /locus_tag="VGM46_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEY4192777.1"
                     /translation="MAPRPAWKGYLKLSLVTCAVELTNVVTHAEKVSFRVLNRKTGNT
                     VKRIYVDAETGKPVEDENEIKG"
     gene            complement(327..833)
                     /locus_tag="VGM46_09045"
     CDS             complement(327..833)
                     /locus_tag="VGM46_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015314781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEY4192778.1"
                     /translation="MGFFSKDIKTLDDLFVHTLRDIYYAENQIEKALPKMIEKATAPE
                     LKAGFEKHLEQTKGHIARLEEVFELHGVKAKQVNCPAIDGILEEADEVSGDIDDKQVL
                     DAALVASAQAVEHYEITRYGTLIAWSNQLGRSDCANILAKNLKEEQDTDRKLTAIAEH
                     GVNLQAAE"
     gene            1245..1394
                     /locus_tag="VGM46_09050"
     CDS             1245..1394
                     /locus_tag="VGM46_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192779.1"
                     /translation="MRFFNHIIGYAAAIREFVSPTYRPERYYMRGPGPACARRSHGFG
                     IGAHG"
     gene            complement(1536..2156)
                     /locus_tag="VGM46_09055"
     CDS             complement(1536..2156)
                     /locus_tag="VGM46_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019169933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY4192780.1"
                     /translation="MYKLYTRPGSGGFVVEAAFALAGEPFELIDVEKSDRPAPAFLEV
                     SPLNQVPVLVLPDGVSITESAAICILLAENCPDAALAPAAGTAERAGFLRFIALMTSV
                     VYPAVLRFYYASRYTTDADGKKAVKQAAIAEMDRGYGVLDAALQGRDWLAGDRLSIAD
                     IYLLMLFSWHPEGARAAQAFPNVERVCASLRKHPAIEALNARHEMW"
     gene            complement(2286..>2650)
                     /locus_tag="VGM46_09060"
     CDS             complement(2286..>2650)
                     /locus_tag="VGM46_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018447828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEY4192781.1"
                     /translation="KKRKPSVHIVAVEPAASAILSGGQPGPHKIQGLGAGFAPKILDT
                     EVYDEIVTVSNEDSVANARLVARLEGVPVGISSGAALQAAVVIGSRPENAGKTMVVVI
                     PSFAERYLSTILFDGLGA"
BASE COUNT          497 a          848 c          809 g          496 t
ORIGIN      
        1 cccttgatct cgttctcgtc ctcgacgggc ttgccggttt cggcatcgac atagatgcgc
       61 ttgacggtat tgccggtctt gcggttcagc acgcggaacg aaaccttctc ggcatgcgtc
      121 accacattgg tcagttcgac cgcgcaggtg accagcgaga gcttgaggta gcctttccag
      181 gcaggacgcg gcgccatcga aatctcccat tgaacttcca gaacggcttg cctgccctgc
      241 ggttccacgg cgaaaaccgg cgggcggttc cacgaggaaa ccggcggggc cgggtttttc
      301 cgaccccgcc ggcaacgata caaatgctat tcggcggctt gcaggttgac cccgtgttcc
      361 gcgatcgccg tcagcttgcg gtcggtatcc tgctcttcct tcaggttttt ggccagaata
      421 ttggcgcagt cgctgcggcc aagctggttg gaccaggcga tcagcgtgcc atagcgcgtg
      481 atttcatagt gctcgacagc ctgtgccgag gcgaccagcg ccgcatcgag aacctgcttg
      541 tcgtcaatgt cgccgcttac ctcgtcggcc tcctccagga tgccgtcgat ggccgggcaa
      601 ttgacctgct tggccttgac gccatgcagt tcgaacactt cctcgagacg ggcaatatgg
      661 cccttggtct gttcaagatg tttttcgaaa ccggctttca gttccggcgc cgtcgccttc
      721 tcgatcattt tcggcagcgc cttctcgatc tggttttcgg cgtaataaat gtcgcgcaac
      781 gtgtgcacga agaggtcgtc cagcgtcttg atgtctttcg aaaagaaacc catggcgttc
      841 gccttccttg cttttggttt tgttcttgct gtttcgggac aggctgccgc tccgtggagc
      901 cgagcaccgt cgggccggtc actccggctc tgcgggtcaa cgtgcccggc gcgagcgagt
      961 tcctgatttt tgaatgcagc tttgagattt ttaatccgcc cggcaaaccg aaacgcaagc
     1021 gcgcctgact gcggttcagg tgaaacaccc gtggcggaaa tgccacaagg acggaaatgg
     1081 cgtccaaaag cgccaaaaga ccccgaatcg gccacattcc agccacgaac caagccggtt
     1141 tggaccgaaa gttaacatca cgttcaccga ctattcacgc ctgccagcta tgatgcaggc
     1201 aattcggaag ggacatccga aagtcgggac agggaccggg tacaatgcgt ttttttaatc
     1261 acatcatcgg ctatgccgcc gccatccgcg aattcgtttc gccgacctat cggccggagc
     1321 gttactacat gcgcggcccc ggcccggcct gcgcgcggcg cagccacggc ttcggcatcg
     1381 gcgcgcacgg atgacgatcg ttcagaccat gccaccgcgc cacgacggtc gcatctgcaa
     1441 accggccgcc gaccagcggg cgacgaccta tcgccggccg ctatcggcgg cgacccgcca
     1501 ccgctaagac ggcgggcagg cgtgcgcagg cacgctcacc acatttcatg ccgggcgttc
     1561 agcgcctcga ttgccggatg tttacgcaac gaagcgcaaa cgcgttccac attcgggaat
     1621 gcctgagcgg cgcgcgcgcc ttccgggtgc cacgaaaaca gcatcagcag atagatatcg
     1681 gcgatcgaca ggcggtctcc cgccagccag tcacggccct gcaaggctgc atcgagcacg
     1741 ccatagccgc gatccatctc ggcgatggcc gcctgcttga ccgccttctt gccgtcggca
     1801 tcggtcgtgt agcgggaggc gtagtagaaa cgcagcaccg ccggatagac gaccgacgtc
     1861 atcaaggcga tgaaacgcag gaagccggcg cgctcggcgg tgccggccgc cggggcaagg
     1921 gctgcatccg ggcaattttc agccagcagg atgcagatgg ccgccgattc ggtgatcgag
     1981 acgccgtccg gcagcaccag caccggaacc tggttcaacg ggctgacttc caggaaggcg
     2041 ggagcaggcc ggtcgctctt ttccacgtcg atcagttcga acggttcacc ggccagcgca
     2101 aacgccgcct cgaccacgaa gccgccggaa ccggggcgcg tataaagctt gtacatgaat
     2161 gtctcccgct accccgcacg gtgcgggccg gcggcgatct gacatgcaag cgggtcgaag
     2221 ctcaagccac cggcgcgcgg gccagaaggc ttttccagcg gtccagcgcg gcgggcgccg
     2281 gggttttacg cgccaaggcc atcaaacagg atggtggaca ggtatcgctc ggcaaaggac
     2341 gggatgacca ccaccatcgt cttgcccgcg ttttcagggc gcgaaccgat caccacggct
     2401 gcctgaagcg ctgctccgga cgagatgccg accggcacgc cctcaaggcg ggcgacaagg
     2461 cgcgcattag cgaccgaatc ctcgttggag acggtgacga tctcgtcata gacctccgtg
     2521 tcgaggatct tcggcgcaaa gccggcccct aacccctgga tcttgtgcgg gccaggctgg
     2581 ccgccggaca ggatcgcgga ggccgccggc tcgacggcga cgatgtgcac cgagggcttg
     2641 cgctttttca
//
LOCUS       DASYCS010000286         2507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2392678,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000286 DASYCS010000000
VERSION     DASYCS010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2392678"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..1166
                     /gene="ribD"
                     /locus_tag="VGM46_09065"
     CDS             42..1166
                     /gene="ribD"
                     /locus_tag="VGM46_09065"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEY4192782.1"
                     /translation="MKDPQNSAQEALDRRFMAAALRLSRRNAGRTATNPSVGTVIVRD
                     DGAGPMIVGTGVTAIGGRPHAETEALAEAGELARGATAYVTLEPCAHHGRTPPCANAL
                     VSAGIARVVGAASDPDARVSGKGYAILRVAGIEVVEKVLAREATEQMAGYLIRSLRKR
                     PEVVLKLALSSDGKIGRRGQGQVTITGEIARREVQMMRVEADAILIGVGTALEDDPAL
                     TVRLPGLENRSPARIVLDRKLRLPESSKLVAGADRVPIYVAMSEDADAGRKAVLERRG
                     VRFIGTETHDGAIALPELMEDLAALGMASVLVEGGAQVASAFLADDLVDRIVLFQGVD
                     EIGAEGIASPIDAAHIPSGFRKLREMRFGEDGCTEWVRDF"
     gene            1166..1783
                     /locus_tag="VGM46_09070"
     CDS             1166..1783
                     /locus_tag="VGM46_09070"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019857614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEY4192783.1"
                     /translation="MFTGIVTDVGTLAAVKPLKEGVGLRIDTAYDPQTIAIGASISCG
                     GVCLTVTGLPESGSNARWFEVEAWEEALRLTTASDWRAGSRINLERALKIGDELGGHI
                     VSGHVDGTATIVARQDEGDAVRFTLEAPRPLAKFIAPKGSVALDGTSLTVNKVEGARF
                     DVLLIHHSLTVTTWGERKVGDRVNLEIDTMARYAARLAEAAKEGL"
     gene            1877..2371
                     /gene="ribH"
                     /locus_tag="VGM46_09075"
     CDS             1877..2371
                     /gene="ribH"
                     /locus_tag="VGM46_09075"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006201904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEY4192784.1"
                     /translation="MAGISEHGKAFIRPKKKAHLLVIEARFHDGLADALLDGATHALQ
                     EAGATFDVVTVPGSLEIPAVITFALDGAGEGATAYDGFVALGTVVRGDTYHFDIVANE
                     SSRALMDLSVQEAVAIGNGILTTENDEQAWTRARRSEGDKGGFAARAALTMIALKERF
                     GAEA"
     gene            2368..>2507
                     /locus_tag="VGM46_09080"
     CDS             2368..>2507
                     /locus_tag="VGM46_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N utilization substance protein B"
                     /protein_id="HEY4192785.1"
                     /translation="MSDTEPTGPVIRQANKRGAARLAAVQALYQMDVAGSGVFEITAE
                     YEA"
BASE COUNT          434 a          789 c          861 g          423 t
ORIGIN      
        1 gggcgaattg aagggtgacg acgaggacgc cgtctagacc gatgaaagat ccgcaaaaca
       61 gcgcgcagga ggccctggac cgccgcttca tggcggccgc gctccggctg tcgcgccgca
      121 atgccggtcg cacggcgacc aatccttcgg tcggcacggt catcgtgcgc gacgacggcg
      181 ccggcccgat gatcgtgggc accggcgtca ccgccattgg cgggcgtccg catgccgaaa
      241 ccgaggcgct tgccgaggcg ggcgaactgg cgcgcggcgc aaccgcctat gtcacgctgg
      301 agccatgcgc ccatcacggc cgcacgccgc cttgcgccaa cgcgctggtc agcgccggca
      361 ttgcccgtgt cgtcggggcg gcgagcgatc cggatgcccg cgtttccggc aagggctacg
      421 ccatattgcg cgtcgccggc atagaggtgg tcgaaaaggt tttggcgcgc gaagcgaccg
      481 aacagatggc cggctatctg attcgatcgc tcaggaagcg gccggaagtc gttctgaaac
      541 ttgcgctttc gagcgatggc aagatcggcc ggcgtgggca ggggcaggtc accatcaccg
      601 gcgagatcgc gcgccgtgag gtgcagatga tgcgggtgga ggccgacgcc attctcattg
      661 gcgttggaac ggcgctggag gacgatcccg cgctgacggt gcggctgccg gggctggaaa
      721 accgctcgcc ggcccgcatc gtgctggacc ggaagctgag gctgcctgaa agttccaagc
      781 tggtcgccgg cgccgaccgc gtgccgattt atgtcgccat gtccgaagat gccgatgcgg
      841 ggcgcaaggc cgtgctggaa cggcgcggtg tccgcttcat cggcaccgag acccatgacg
      901 gagccatcgc cttgccggaa ctgatggaag atctggcggc gctcggcatg gcgagcgttc
      961 tggtcgaagg cggcgcgcag gttgccagcg cctttctggc cgacgatctg gtggaccgca
     1021 tcgtgctgtt tcaaggcgtc gacgagatcg gcgcggaagg cattgcctcg cccatcgacg
     1081 ccgcccatat cccgtccggc tttcgcaagc tgcgggaaat gcgcttcggt gaggatggct
     1141 gcaccgagtg ggtaagggat ttctgatgtt cactggaatt gtcaccgacg ttggcacgct
     1201 ggcagccgtc aagccgttga aggagggcgt cggcctgcgc atcgacacgg cctacgaccc
     1261 gcaaacgatt gccatcggcg cgtccatttc ctgcggcggc gtctgcctca ccgtcaccgg
     1321 gctgccggaa agcggctcca acgcgcgctg gttcgaggtc gaggcttggg aggaggcgct
     1381 gcggctgacg acggcgtcgg attggcgggc cggctcgcgc atcaacctgg agcgcgcgct
     1441 gaagatcggc gacgaacttg gcggccatat cgtttcaggc catgtcgacg gcaccgccac
     1501 catcgttgcg cggcaagacg aaggcgatgc ggtgcgcttc acgctggaag cgccgcgtcc
     1561 attggcgaag ttcattgcac cgaagggttc cgtcgcgctg gacggcacct cgcttacggt
     1621 caacaaggtc gagggcgcgc gcttcgacgt gcttttgatc catcattcgc tgaccgtcac
     1681 cacctggggc gaacgcaagg ttggcgaccg cgtcaatctc gagatcgaca ccatggcgcg
     1741 ctatgctgcg cgtctcgccg aggcggcgaa ggaaggtctt tgaggcggcc tgccattttt
     1801 gactggaatg tctatactgc ggcgcttgcg atattgagaa cttcggccta agtcacccgc
     1861 tctctggaga gcattcatgg ctggcatatc ggaacacggc aaggcgttca tccgcccgaa
     1921 aaagaaggcg caccttcttg tcatcgaggc gcgcttccac gacggtctgg ccgacgcgct
     1981 gctggatggc gcgacgcatg cgctgcaaga agcgggggcg actttcgatg tcgtcaccgt
     2041 tcctggctcg ctggaaatcc cggctgtcat tacctttgcg ctggacggcg cgggcgaggg
     2101 cgcaaccgcc tatgacggct tcgtggcgct cggcaccgtc gttcgcggcg acacctatca
     2161 cttcgacatc gtcgccaatg aatcgagccg tgcgctgatg gacctttccg tgcaggaagc
     2221 ggtcgccatc ggcaacggca tccttaccac cgaaaacgac gagcaggcat ggacgcgggc
     2281 caggcgcagc gaaggcgaca agggcggctt tgccgcccgc gccgccctga ccatgatcgc
     2341 gctcaaggaa cgcttcggag ccgaagcgtg agcgacaccg aacccaccgg cccggtcatc
     2401 cgtcaagcca acaagcgggg cgcggcccgg cttgccgccg tgcaggcgct ctaccagatg
     2461 gatgtcgccg gcagcggcgt gttcgagatc accgccgaat acgaggc
//
LOCUS       DASYCS010000287         5299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_363226,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000287 DASYCS010000000
VERSION     DASYCS010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 5299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5299
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363226"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            238..966
                     /locus_tag="VGM46_09085"
     CDS             238..966
                     /locus_tag="VGM46_09085"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019863033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEY4192786.1"
                     /translation="MSTLTLIPARMASTRLPGKPLADIAGSPMIVQVARRAAEAGLGR
                     VVVATDTVAVAEAVRAHGFEAVMTRSDHESGSDRIFEALAALDPHGAVDTVVNVQGDL
                     PTIDPAIIHAVLKPFDDQAVDIATLCVEIVRDDEKTNPNVVKVVGSPLSASRLKALYF
                     TRATAPWGEGPLYHHIGLYAYRRPALERFVALKPSPLERRERLEQLRALEAGMRIDAE
                     IVQSVPLGVDTPEDLERARKILSN"
     gene            979..1860
                     /locus_tag="VGM46_09090"
     CDS             979..1860
                     /locus_tag="VGM46_09090"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEY4192787.1"
                     /translation="MPEKATKEKTNRIAFQGEPGANSDTACRNVYPKMEPLPCPSFED
                     AFNAVETGKADLAMIPIENTIAGRVADIHHLLPESKLHIVGEYFLPIHFQLMVLPGVK
                     REEIKTIHSHIHALGQCRKYIRKNGWKPIVAGDTAGAAKLVSEMKDRSMGALAPALAS
                     TLYGLDVIEENVEDTENNITRFVVLSKEKAWAQRTGKHAATMTTFVFRVRNVPAALYK
                     AMGGFATNGVNMTKLESYQLDGGFTATQFYADIEGHPDDAHVARALEELRFFSREVRI
                     LGVYAANEYRAAQGAGY"
     gene            complement(1868..2812)
                     /gene="nudC"
                     /locus_tag="VGM46_09095"
     CDS             complement(1868..2812)
                     /gene="nudC"
                     /locus_tag="VGM46_09095"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013527934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HEY4192788.1"
                     /translation="MVFPLFDAPLREPSQFVGFAGNRLDRQSENRSDDCVEKALADPT
                     VRIMLIRDGKLLLKPQGEAFDAWFGAAESMTYGAALAEAILLGFSDAGPVLAAPTTLD
                     LDHLPETVKALDYRSVYTQGLVDEAAVGALAQAASLLAWNVSHRFCSKCGAQSAMHAG
                     GYRRRCSACGTEHFPRTDPVAIMLTATPEKCLLGRSRHFAPGMYSALAGFIEPGETIE
                     DAVRRETLEEAGIRLGRVVYHASQPWPFPYSLMIGCFGEPLNDDIHADLNELEDCRWF
                     FRDEVRQMLAREHPGGLVTPPKGAIAHHLIQAWVDAEG"
     gene            complement(2850..3269)
                     /locus_tag="VGM46_09100"
     CDS             complement(2850..3269)
                     /locus_tag="VGM46_09100"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013527933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEY4192789.1"
                     /translation="MMPGLKAGFTLDARLEADSEQLMWLGLCELRVMNDSRWPWLVLV
                     PQRPGAEEIHDMTPLDQAMLTFEVNMVASALKRVSGCTKINTGALGNIVRQLHIHVVA
                     RSEGDPAWPGPVWGHGVREPYQRSDLRRFAETVRTAL"
     gene            3422..3519
                     /gene="ffs"
                     /locus_tag="VGM46_09105"
     ncRNA           3422..3519
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VGM46_09105"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            3685..>5299
                     /locus_tag="VGM46_09110"
     CDS             3685..>5299
                     /locus_tag="VGM46_09110"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003524312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEY4192790.1"
                     /translation="MSEAGPSDASPSHVSPVDAGPVVGGAKAAAYRVLARKYRPANFS
                     ELIGQEPMVRTLTNAFSTGRIAQAWMLTGVRGVGKTTTARILARALNYKTATIDQPSV
                     DLTVPGEHCQAIMEGRHVDVIEMDAASHTGIDDIRDIIERVRYAPVSARYKVYIIDEV
                     HMLSNQAFNGLLKTLEEPPPHVKFIFATTEIRKVPITVLSRCQRFDLRRIDAGALVAH
                     LSAIAAKEGIAVDGEALAMIARAAEGSARDSLSILDQAIAHGGGMVGAAEVRAMLGLA
                     DRARIVDLFEHVMKGDVAAALAEFRSQYDTGADPATVLTDLAEFNHLVTRLRFVPDAI
                     EDASLSQEERSRGAAMAKALSVRVLSRTWQMLLKGIPEVQSSSRPVSAAEMVLIRLAH
                     AADLPTLDEALKTLEGGTFSPAPEPRTNGTPISAAPASFNSPSSSSFNDSGPAPQARM
                     PASGGGGAQAMRLVAATPAPEPANFIAPPEPAAAEQPLVPVKSLADIAALADTHRDVA
                     FKVLLKRCVRPVRIEPGRIEVSLTADAPKL"
BASE COUNT          979 a         1784 c         1641 g          895 t
ORIGIN      
        1 gccagcaatc cgccgatcag cttgttgatt tcgaaagagt ccattcgccc caggctccct
       61 ttgtccggca cgaccgatca tccagccttg ccgtgagtat cgcgacccac cccgcggagc
      121 cggccaaatc cgggcggaaa ctaggtcttt tgctcggacg ttgcaacacc tataaggtca
      181 cttccagtga gactttttga cactttcatt cttcttcgcg aacgatccca tcagcccatg
      241 tccacgctta ccctcatccc ggcccgcatg gcctctaccc gcctgccggg caagccgctg
      301 gccgacattg ccggcagtcc gatgatcgtg caggttgcgc gccgcgccgc cgaggccggg
      361 cttggccggg tagttgtcgc caccgatacg gtagccgtcg cggaagccgt gcgcgcgcac
      421 ggcttcgagg cggtgatgac gcgaagcgac catgaatccg gctccgaccg cattttcgag
      481 gcgctggcgg cgctcgatcc gcatggcgcg gtcgacacgg tggtcaatgt acagggcgat
      541 ctgccgacca tcgaccccgc catcattcat gccgtgctga agccgttcga cgaccaggcg
      601 gtcgatatcg ctacgctatg cgtggaaatc gtgcgcgacg acgagaagac gaatccgaac
      661 gtggtcaagg tcgtcggctc gccgctgtcg gcctcgcggc tgaaggcgct ttatttcacc
      721 cgcgccaccg cgccgtgggg cgaagggccg ctctatcatc atatcggcct ctacgcctac
      781 cgccgcccgg cgctggagcg cttcgtcgcc ttgaagccgt cgccgctgga gcggcgcgag
      841 cggctggagc agttgcgcgc gcttgaggcc ggcatgcgca tcgacgccga aatcgtccaa
      901 tcggtgccgc tcggcgtcga cacgcccgaa gacctcgaac gcgccagaaa aattctttca
      961 aactgaaaag ccggacccat gcctgaaaaa gcgaccaagg aaaaaaccaa ccggatagcc
     1021 ttccagggcg agccgggggc caactcggac accgcctgcc gcaacgtcta tcccaagatg
     1081 gagccgctgc cctgcccgag cttcgaggac gccttcaacg cggtcgagac gggcaaggcc
     1141 gacctcgcca tgatcccgat cgagaacacg atcgctggcc gcgtcgccga cattcaccac
     1201 ctgctgccgg aatcgaagct ccacattgtc ggtgaatatt tcctgcccat ccatttccag
     1261 ctgatggtgc tgcccggcgt caagcgcgaa gagatcaaga ccatccacag ccacatccat
     1321 gcgctcggcc aatgccgcaa atatatccgc aagaacggct ggaagccgat cgttgccggc
     1381 gatacggcgg gcgcggccaa gctggtcagc gagatgaagg accgcagcat gggcgcgctg
     1441 gcgccggcgc tggcgtccac cctctacggc ctcgacgtga tcgaggaaaa tgtcgaggac
     1501 accgaaaaca acatcacccg tttcgtcgtt ttgtcgaagg aaaaggcctg ggcgcaacgc
     1561 accggcaagc atgccgcgac gatgacgacc ttcgtctttc gcgtgcgcaa cgtgccggcg
     1621 gcgctctaca aggcgatggg cggctttgcc accaacggcg tcaacatgac caagctggaa
     1681 agctaccagc tcgatggcgg cttcaccgcc acgcaattct atgccgatat cgagggacat
     1741 ccggacgacg ctcatgtggc gcgcgcgctt gaagaactgc gcttcttttc gcgcgaagtg
     1801 cgcattctcg gcgtctacgc ggccaacgaa taccgcgccg cgcaaggcgc cggctactga
     1861 ccgcgaccta gccttcggcg tcgacccatg cctggatgag gtgatgggcg atagcgcctt
     1921 tcggcggcgt gacaaggccg cctggatgct ccctggccag catctggcgc acttcgtcgc
     1981 ggaaaaacca gcggcagtcc tccaactcgt ttaagtcggc gtggatatcg tcgttgagcg
     2041 gctcgccgaa gcagccgatc atcagcgaat agggaaacgg ccatggctgg ctggcatggt
     2101 agacgacgcg gccaagcctg ataccggctt cttccagcgt ctcgcggcgc accgcatcct
     2161 cgatcgtctc gcccggctcg ataaagccgg caagcgctga atacattccc ggcgcaaagt
     2221 gccggctacg gccgagcagg catttttccg gcgtcgccgt cagcatgatg gcgaccgggt
     2281 ctgtacgcgg aaaatgttcg gtgccgcaag cggagcacct gcgccggtag ccgccggcat
     2341 gcatcgccga ttgcgcgccg catttgctac agaagcggtg gctgacattc catgccagca
     2401 gcgaggccgc ctgcgccaag gcgccgaccg ccgcctcgtc gaccagacct tgcgtataga
     2461 cggaacggta atcgagcgcc ttgacggttt cgggcaggtg gtcgagatca agggtggtcg
     2521 gagcggcaag cacggggccg gcatccgaaa aaccaagcag gatcgcttcc gccagcgccg
     2581 cgccatacgt catgctctcg gcggcgccga accacgcgtc gaacgcctcg ccttgcggtt
     2641 tcagcaaaag ctttccgtcg cggatgagca tgattcggac cgtgggatcg gccagcgcct
     2701 tctcgacgca gtcgtcggag cggttttcgg actgccggtc gagccggttg ccggcaaagc
     2761 ccacgaactg gctgggttcg cgcaacggcg catcgaacag aggaaagacc atttatcgca
     2821 gactcacacg ctgggcaggt tcgggcgctt cataacgcgg tgcggacggt ttcggcaaac
     2881 cggcgcagat cggaacgctg atacggctcg cgcacgccgt ggccccagac aggccccggc
     2941 caggcgggat cgccttccga gcgcgcgact acatggatat ggagctggcg cacgatgttg
     3001 ccgagcgcgc cggtattgat cttggtgcag ccgctcaccc ttttcagcgc agaggccacc
     3061 atattgacct cgaaagtcag catggcctgg tcgagcggcg tcatgtcgtg gatttcttcc
     3121 gcgcccggcc gctgcggcac cagaaccagc cagggccagc gactgtcgtt catgacgcgc
     3181 agctcgcaca ggccaagcca cattaattgt tcgctgtcgg cttccagacg cgcgtcaagc
     3241 gtgaatccgg ccttcaggcc cggcatcatc gatttgtccc tgctttgaat tcgtgaccgc
     3301 aatcttttga agctattgcg gcattccccg cgccgcaagc gtttcattgg cgctggccgg
     3361 caatgtgcgg cgatttcttt gcgcccttgc atttcgcctc cgaattgccg atatggaaac
     3421 cgggaggttg gtggtggacg atccactcgc caaccgggtc aggtccggaa ggaagcagcc
     3481 ctaacgagcc cggaacgggt catgttccag cctcccacct cttcgcaacc agccgaccgg
     3541 accgttgccg ccagattgcg tgaccaaggc gcggctggct aaagcgccct cgccaggcca
     3601 tagccgcatt gacgggcaag ccggtcgcgg cgaaactctg tattggaatt ggccggacga
     3661 gacggccggg agcaaaacga gcggatgagc gaagccggac caagcgatgc aagcccaagc
     3721 catgtaagcc cagtcgatgc aggcccagtg gtcggcggcg ccaaggctgc cgcctaccgg
     3781 gtgctggcgc gcaaatatcg cccggcgaat ttctccgaac tgatcggcca ggagccgatg
     3841 gtgcggacgc tgaccaacgc gttttccacc ggccgcatcg cccaggcgtg gatgctgacg
     3901 ggcgtgcgcg gcgtcggcaa gaccaccacc gcccgcatcc tcgcccgcgc gctgaactac
     3961 aagaccgcca ccatcgacca gcccagcgtc gacctgaccg tgcccggcga gcattgccag
     4021 gcgatcatgg aaggccgcca tgtcgatgtg atcgaaatgg acgccgcctc gcataccggc
     4081 atcgacgaca ttcgcgatat tatcgagcgg gtgcgctacg cgccggtttc ggcccgctac
     4141 aaggtctaca tcatcgacga agtgcacatg ctgtccaacc aggccttcaa cggcctgctg
     4201 aagacgctgg aagagccgcc gccgcacgtc aagttcatct tcgccaccac cgaaatccgc
     4261 aaggtgccga tcaccgtcct gtcgcgctgc cagcgcttcg acctgcgccg catcgatgcc
     4321 ggcgcgctgg tggcgcacct ttccgccatc gcggccaagg aaggcatcgc ggtcgacggc
     4381 gaggcgctgg cgatgatcgc gcgcgcggcg gaaggctcgg cgcgcgattc gctttccata
     4441 ctcgaccagg cgatcgccca tggtggcggc atggtcgggg cggcggaagt gcgcgccatg
     4501 ctcggcctcg ccgaccgcgc ccgcatcgtc gatttgttcg agcatgtgat gaagggcgac
     4561 gtggcggcgg cactcgccga gttccgctcg caatatgaca ccggcgcgga cccggccacc
     4621 gtcctgaccg acctggccga gttcaaccac ctcgtcaccc gcctgcgttt cgtgccggac
     4681 gccatcgagg acgcctcgct gtcgcaggaa gagcgcagcc ggggcgctgc aatggccaag
     4741 gccttgtccg tgcgcgtcct ttcgcgcaca tggcagatgc tgctgaaggg aattcccgag
     4801 gttcaaagct ccagccgccc ggtcagcgcg gccgaaatgg tgctgatccg cctcgcccac
     4861 gccgccgacc tgccgacgct ggacgaagcg ctcaagacgc tggaaggcgg gactttttcg
     4921 cccgcgccgg agccgcgcac caacggcacg ccgatttcgg ccgctcccgc ttccttcaat
     4981 tccccgtcca gttcctcctt caacgatagc ggccctgcac cgcaggcgcg aatgccggca
     5041 agcggcggcg gcggcgcgca ggcgatgcgg ctggtggcgg ccacgcccgc gccggaaccg
     5101 gcaaatttca tcgcgccgcc ggaacccgcg gcggcggagc agccattggt gccggtcaag
     5161 tctctggccg acatcgccgc ccttgcggac acgcatcgcg acgttgcctt caaggtgctt
     5221 ttgaagcgtt gcgtgcgccc tgtccgcatc gagcctggcc gcatcgaagt cagcctgact
     5281 gccgacgcgc ccaagcttc
//
LOCUS       DASYCS010000288         4323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3699530,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000288 DASYCS010000000
VERSION     DASYCS010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3699530"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /locus_tag="VGM46_09115"
     CDS             complement(<1..1122)
                     /locus_tag="VGM46_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015319232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional rhamnulose-1-phosphate
                     aldolase/short-chain dehydrogenase"
                     /protein_id="HEY4192791.1"
                     /translation="MLDKRSGSRLANLWEDARAAAMSEPERLVYRSNILGSDKRVTNY
                     GGGNTSSKIWQADPLTGEQVEVLWVKGSGGDSASIKLDGFSTLYMEKLRALKGLYRGL
                     EHEDEMVGYLPHCTFNLNPRAASIDTPLHAYVPHAYVDHMHPDAIIAIAASKESKALT
                     AEIFGGEIGWLPWKRPGFELGLWLEKFCLENPAAKGVILESHGLFTWGDTPGECYETT
                     IAVINRATEWLEQQSQGKPAFGGPVAKPLDPATRRAIAARLMPAIRGLVSEKSHKLGH
                     FDDSDAVLEFVNSARLGELAALGTSCPDHFLRTKIRPLVIEFDPAKPDVDAVIARLAD
                     DIAAYRTGYQAYYDRCKHPGSPAMRDPNAVVYLMPGVGMF"
     gene            1307..2116
                     /locus_tag="VGM46_09120"
     CDS             1307..2116
                     /locus_tag="VGM46_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011974434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEY4192792.1"
                     /translation="MHEKERHRIILSAVQEKPVATVQEMVDLTDSSEATIRRDIAALH
                     VQKRLRRVRGGAEALSPPSFVGLAGRPFSVNETINAAQKRAIARAAAELCKDGEPIII
                     NGGTTTFQMVHFLTNRRMPIFTNSFPIAEHLLKHSKNTVMLSGGTIYREQNIILSPFD
                     NDVTRNFYARRMFMGAQGLGPLGLMEGDPLLIQAEQKLVDQADELVVLIDSSKFRRRS
                     SLILCGLSRIATVITDDGIEDREAKMLEGAGVALIVAGKAAGELEESSLQA"
     gene            2170..3165
                     /gene="rhaS"
                     /locus_tag="VGM46_09125"
     CDS             2170..3165
                     /gene="rhaS"
                     /locus_tag="VGM46_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0033296 - rhamnose binding [Evidence IEA];
                     GO_process: GO:0015762 - rhamnose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnose ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY4192793.1"
                     /translation="MTIWKKLLATVAISAAMMTNAAYADNAKIALVVKSLGNGFFDAA
                     NKGAQEAAKELGDVDVIYTGPTKATAEAQIEVINSLIAQKVDAIAISANDADALVPAL
                     KKAMERGITVISFDSGVAPEGRQLHLNPSDTNLIGETIIKLAADYLPEGGDVAILSAA
                     STATNQNAWIDAAKKALPEKFPKIKLVATVYGDDDSAKSTDEAKGLLKSYPNLKAIIA
                     PTTVGVVAAAQVVTDENLIGKVNVTGLALPSEFKKFIDNGASQAVALWNPIDLGYSAI
                     YLAHDLAVKKEEAKPGATLSIGRVGKVTLDDTNSAAMAPPFQFDKSNIEEFSKIY"
     gene            3284..>4323
                     /locus_tag="VGM46_09130"
     CDS             3284..>4323
                     /locus_tag="VGM46_09130"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEY4192794.1"
                     /translation="MPDHPHPASTVSDPPRKGEEKAAPRLTLTGIGKSFPGVRALHDV
                     SLSLYPGEVTALIGENGAGKSTLVKIMTGIYQPDAGVISVDGQAVTLSSAHAAFAHGI
                     TAIHQETVLFDDLSVAENIFLGHAPRNRLGLLDWSTMRANAREVLTTMRAGHIDPDTR
                     LKDLGIANKHLVAVARAMSIDAQIVIMDEPTAALSMKEIEELFLLVEFLKSAGKAILF
                     ISHKFDEIFAIADRYCVFRDGERVGEGLLKDTSQSAIVKLMVGRPVDQIFPHRNSEIG
                     APVLAVSGLSHPTEFDDIGFELHKGEILGFYGLVGAGRSEVMQAIAGITRTSGGSITL
                     DGKAISPKSAADS"
BASE COUNT          847 a         1346 c         1357 g          773 t
ORIGIN      
        1 gaacatgccg acgcccggca tcaggtagac taccgcattg ggatcgcgca tggcaggcga
       61 gcctggatgt ttgcagcggt cgtaataggc ctggtagccg gtgcggtagg ccgcgatgtc
      121 gtcggcaagg cgcgcaatga ccgcatcgac atccggtttg gccggatcga actcgatgac
      181 cagcgggcgg atcttggtgc gcaggaaatg gtcggggcac gaggtgccaa gggctgcaag
      241 ttcgccaagc ctggccgagt tgacgaactc cagcaccgcg tcggaatcgt cgaaatggcc
      301 aagcttgtgc gatttttccg agaccagtcc gcgaatggcc ggcatcagcc tggcggcgat
      361 ggcgcggcgc gtggcggggt cgagcggctt tgcaaccggc ccgccaaacg ccggcttgcc
      421 ctgcgactgc tgttcgagcc attcggtcgc ccggttgatg acggcaatcg tcgtctcgta
      481 gcattcgccg ggcgtatcgc cccaggtgaa caggccgtgg ctttcgagga tgacgccctt
      541 ggcggcgggg ttttccaggc agaatttctc cagccacagg ccaagctcga agcccggcct
      601 tttccacggc agccagccga tttcgccgcc gaaaatctcc gccgtcagcg ccttcgattc
      661 cttcgacgcg gcaatcgcga tgatggcgtc cgggtgcatg tggtcgacat aggcgtgcgg
      721 cacataggca tgcagcggcg tatcgatgga ggcggcgcgc gggttaaggt tgaaggtaca
      781 gtgcggcagg tagccgacca tctcgtcttc atgttcgagg ccgcgataca ggcccttcag
      841 cgcgcgcagc ttttccatgt agagcgtgga gaagccgtcg agcttgatcg aggcgctgtc
      901 gccgccggaa cccttgaccc acagcacctc cacctgctcg ccggtcagcg gatcggcctg
      961 ccatatcttg gacgaggtat tgccgccgcc ataattggtc acgcgcttgt cggaaccgag
     1021 gatgttggag cggtagacca gccgttccgg ctcgctcatc gcggccgccc tggcgtcctc
     1081 ccacagattg gcaaggcgcg agccggagcg cttgtcgagc atgatcggtt cctcccggtg
     1141 gcgctcaaga tggcgcgata tgcggggcaa gttttcacga ttagcggtag tcgtcaatca
     1201 aaaacgatca aatgtaatca tattgcggcg cacaatggaa ttatatgatt gatgttgatt
     1261 gacactgcgt gaccgccatg cctagatgaa gcggcaggga ggaacgatgc acgagaaaga
     1321 gcgccacagg atcattctgt ccgccgtcca ggaaaagcct gtcgcgacgg tgcaggaaat
     1381 ggtcgacctg accgattcct cggaggcgac gatccgccgc gacatcgcgg ccctgcatgt
     1441 gcaaaaacgc ctgcgccggg tgcgcggcgg cgccgaggcg ctttctccgc catccttcgt
     1501 cggcctggcc ggccggcctt tctcggtcaa cgaaaccatc aacgcggcgc agaagcgcgc
     1561 tatcgcccgc gcggcggcgg agctttgcaa ggacggcgag ccgatcatca tcaatggcgg
     1621 caccacgacc ttccagatgg tccatttcct gaccaaccgg cgcatgccga tctttaccaa
     1681 ctcgtttccg attgccgagc acctgctcaa gcactccaag aatacggtga tgctttccgg
     1741 cggcaccatc taccgcgagc agaacatcat cctgtcgccc ttcgacaatg acgtgacgcg
     1801 caatttctat gcgcgccgca tgttcatggg cgcgcagggg ctggggccgc tggggctgat
     1861 ggaaggcgat ccactgctta tccaggccga acagaagctg gtcgaccagg ccgacgaact
     1921 ggtggtgctg atcgattcgt cgaagttccg ccgccgctcc agcctgatcc tgtgcggcct
     1981 gtcgcggatc gccaccgtca tcaccgacga cggcatcgag gacagggaag ccaagatgct
     2041 ggaaggcgcc ggcgtggcgc tgatcgtcgc cggcaaggcg gcaggcgaac tggaggaatc
     2101 ttcgctgcaa gcctgaacga ttcaggctgc ggtgctgatg ggaatgcaac gctcaaggga
     2161 ggtaaaccta tgactatctg gaagaaattg ctggccacag tggcaatctc cgccgccatg
     2221 atgacgaatg ccgcctatgc cgacaacgcc aagatcgcgc tggtggtgaa gtcgctcggc
     2281 aacggcttct tcgacgccgc caacaagggc gcgcaggaag cggccaagga actcggcgat
     2341 gtcgacgtga tctacaccgg cccgaccaag gcgaccgccg aagcgcagat cgaggtgatc
     2401 aactcgctga tcgcccagaa ggtcgatgcc atcgccattt ccgccaacga cgccgacgcg
     2461 ctggttcccg cgctgaagaa ggcgatggag cgcggcatca ccgtcatctc gttcgattcg
     2521 ggcgtggcgc cggaaggccg gcagcttcac ctcaacccgt ccgataccaa tctgatcggc
     2581 gaaaccatca tcaagctggc agccgactat ctgcccgagg gcggcgatgt cgccatcctg
     2641 tcggcggctt ccaccgccac caaccagaac gcctggatcg acgccgccaa gaaggcgctg
     2701 ccggagaagt tcccgaagat caagctcgtc gccaccgtct atggcgatga cgattcggcc
     2761 aagagcacgg acgaggccaa gggcctgttg aagtcctacc cgaacctgaa ggcgatcatc
     2821 gcgccgacca cggttggcgt cgtcgccgcc gcccaggtgg tgaccgacga gaacctgatc
     2881 ggcaaggtaa acgtcaccgg cctggcgctg ccttcggagt tcaagaagtt catcgacaat
     2941 ggcgcaagcc aggcggtcgc tttgtggaac ccgatcgatc tcggctattc cgccatctat
     3001 ctggcgcacg accttgcggt gaagaaggaa gaagccaagc caggcgccac gctttccatc
     3061 ggccgcgtcg gcaaggtgac gctcgacgac accaactcgg ccgcgatggc tccgcccttc
     3121 cagttcgaca agtcgaacat cgaagagttc tccaagatct attgatcgct ggactgcctg
     3181 ccctctccct tgcggggagg gccggtggat gaatggaacg aagtgacgct gtaagacggg
     3241 gtgggggtca tgtttttaca cgcgccgtct gccggaaaca aaaatgcctg atcaccccca
     3301 ccccgcttca actgttagcg accctcctcg caagggcgag gaaaaggccg cgccccggct
     3361 gacgctgacc ggcatcggca agagtttccc cggcgttcgc gcgctccacg atgtctccct
     3421 gtcgctttat ccgggcgagg tgaccgcgtt gatcggcgaa aacggcgccg gcaagtccac
     3481 gctggtcaag atcatgaccg gcatctacca gccggacgcc ggcgtcatct ccgtcgacgg
     3541 ccaggcggtc acgctgtctt ccgcgcatgc cgcctttgcg cacggcatca ccgcgatcca
     3601 tcaggaaacc gtgctgttcg acgatttgag cgtggcggag aacatctttc tcggtcatgc
     3661 gccgcgcaac cggctcggcc tgctcgactg gtcgaccatg cgggccaatg cgcgcgaggt
     3721 gctgaccacc atgcgcgccg gccatatcga tcccgatacg cggctgaagg accttggcat
     3781 cgccaacaag catctggttg cggtggcccg cgccatgtcg atcgacgcgc agatcgtcat
     3841 catggacgag ccgacggcag ccctttcgat gaaggagatc gaggagcttt tcctgctggt
     3901 cgaattcctc aagagcgccg gcaaggcgat cctgttcatc agccacaaat tcgatgagat
     3961 attcgccatc gccgaccgct actgcgtctt ccgcgacggc gagagggtgg gtgaggggct
     4021 gctgaaggac accagccaaa gcgccatcgt caagctcatg gtcgggcgtc cggtcgatca
     4081 gatattcccg catcgcaaca gcgaaatcgg cgcgccggtg cttgccgtca gcgggctttc
     4141 gcatccgacc gagttcgacg atatcggctt tgaactgcac aagggcgaaa tcctcggctt
     4201 ttacgggctg gtcggcgcgg ggcgcagcga ggtgatgcag gcgattgccg gcataacgcg
     4261 caccagcggc ggttcgatca cgctggacgg caaggcgatt tcgccgaaat cggccgcgga
     4321 ttc
//
LOCUS       DASYCS010000289         7913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1672418,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000289 DASYCS010000000
VERSION     DASYCS010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 7913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7913
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1672418"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="VGM46_09135"
     CDS             complement(<1..648)
                     /locus_tag="VGM46_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase N-terminal
                     domain-containing protein"
                     /protein_id="HEY4192795.1"
                     /translation="MTAKWNFWIDRGGTFTDIIGRDPDGGLHPRKLLSENPEAYADAA
                     IAGIRGLLGLEPGEPVPVDLIGDIRMGTTVATNALLERKGDRVLLLITKGFRDALAIA
                     YQARPDIFAKEIILPERLYERVVEVDERIRADGCIERLLDIADVLPAIKQAKADGIDA
                     VAIVFMHAWKYPGHEQAVAKLCRKLGFGQVSVSHEASPLVKLIGRGDTTVVDAYLS"
     gene            932..2143
                     /locus_tag="VGM46_09140"
     CDS             932..2143
                     /locus_tag="VGM46_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEY4192796.1"
                     /translation="MKITDVKTFVVGNPPPGIGGKYFIFVKLTTDGGVVGYGEAYNAT
                     FSAHITARMIEDMAERYLVGHDPHDIETLFRRCYSSGFTQRPDVSAMGCFSALEIACW
                     DIIGKEAGKPIYKLLGGQVHEKLRSYTYLYPHSGSVHTEDVDGKNVYNDPDLAAECAA
                     LYVEQGFNAVKLDPAGPYTAFDGHQPRLADIDLSARMMKAIREAVGNRADILFGTHGQ
                     FTASGALRMAAAIEPYDPLWFEEPVPPDMPEVMAQVARGTRIPIATGERLTTKFEFAR
                     VIENRAAAILQPDLGRSGGILETKKIAAMAEAYHIQIAPHCYCGPIVGAANVQVAATL
                     PNFLILESLKQWDSFHAKLLKQKIEWQDGNVIPSKEPGLGVELDEAVCEAHPYEGKAL
                     HLEMKLDPLFP"
     gene            complement(2341..2652)
                     /locus_tag="VGM46_09145"
     CDS             complement(2341..2652)
                     /locus_tag="VGM46_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008877014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protamine-2 (modular protein)"
                     /protein_id="HEY4192797.1"
                     /translation="MDRRSFLTGMLGLAGAAALIGVAQPDSTMAGVMPGSDGILDELD
                     EPATEAEPVQYRRDRHWRRDNRRHNRRHRRRVWRRVCSSYWRRGRRHVRCYRRRVWVW
                     G"
     gene            complement(2825..2901)
                     /locus_tag="VGM46_09150"
     tRNA            complement(2825..2901)
                     /locus_tag="VGM46_09150"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2865..2867),aa:Met,seq:cat)
     gene            3129..4094
                     /locus_tag="VGM46_09155"
     CDS             3129..4094
                     /locus_tag="VGM46_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008124408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY4192798.1"
                     /translation="MIPRIAVLGCGYWGSNHIRTLKALGALYAVSDANPARAEGFASE
                     QDCLAIAPDALFANPDIDAVVLALPPQFHADMAVRAVENGKDVLVEKPIALTVPDAER
                     AVKAARDNGRVFMVGHVLRFHPAFETLKALIDKGELGEVRYIHSHRLGLGKFHTENDA
                     LWDLAPHDLSMILAITGTEPLEVHGEGAAILDNLSDFAHLHMRFPHGLSGHLFASRLN
                     PYRERRLTVVGTKAMAVFDDVEPWERKLAVYRHAVWQDSGQWAFTTNEPTYVAVKQGM
                     PLTRELEHFIQCIQTRAEPRTDGQEAITVLKVLTAGTVKHDKRRR"
     gene            complement(4163..5359)
                     /gene="mnmA"
                     /locus_tag="VGM46_09160"
     CDS             complement(4163..5359)
                     /gene="mnmA"
                     /locus_tag="VGM46_09160"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEY4192799.1"
                     /translation="MTMNSLDLPARPEDTRIVVAMSGGVDSSVVAGLLKHEGYDVVGV
                     TLQLYDHGAATHRAGSCCAGQDIDDARRVAETLGIPHYVLNYEERFRKAVIDPFATSY
                     MAGETPIPCVACNQTVKFADLLTTAQELEADALATGHYIRSRANGAHRSLYRPVDADR
                     DQSYFLFATTQAQIDYLRFPLGHLPKQQVRAIAEEMGLSVAAKHDSQDICFVPQGKYS
                     DVIAKLRPTATIPGNIVHIDGRVLGHHEGILRYTIGQRRGIGIASGEPLYVVHLDADN
                     ARVVVGPREALETHKIYLRDMNWLGDGDLSDIAHDGLELFAKVRSTRPPRPAVLRHRD
                     GAIWVELSDGESGIAPGQACVLYSDDGNEARVFGGGFIARSERSADAEAMLDRLAANP
                     ANIAAE"
     gene            5637..5912
                     /locus_tag="VGM46_09165"
     CDS             5637..5912
                     /locus_tag="VGM46_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010659559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1153 domain-containing protein"
                     /protein_id="HEY4192800.1"
                     /translation="MTDLIRPRVKYVIGPDGSPLTIADLPPTNTRRWVIRRKAEVVAA
                     VRGGLLSLEEACQRYKLTTEEFLSWQASIDEYGLAGLRTTRIQQYRH"
     gene            complement(6340..6717)
                     /locus_tag="VGM46_09170"
     CDS             complement(6340..6717)
                     /locus_tag="VGM46_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HEY4192801.1"
                     /translation="MKSRENLVRLKQFQVNEKRRQLLQLDMMIAEFERMATELELQIA
                     AEEKKAGITDINHFAYPTFATAARLRRDNLRNSQSDLAQQRSAAESILAEAEAELSKA
                     EMLESRDIKVRDVEMVARSAMIG"
     gene            7176..7871
                     /gene="ctrA"
                     /locus_tag="VGM46_09175"
     CDS             7176..7871
                     /gene="ctrA"
                     /locus_tag="VGM46_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002964699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell cycle two-component system response
                     regulator CtrA"
                     /protein_id="HEY4192802.1"
                     /translation="MRVLLIEDDSATAQSIELMLKSESFNVYTTDLGEEGVDLGKLYD
                     YDIILLDLNLPDMSGYEVLRTLRLSKVKTPILILSGMAGIEDKVRGLGFGADDYMTKP
                     FHKDELVARIHAIVRRSKGHAQSVITTGELVVNLDAKTVEVSTQRVHLTGKEYQMLEL
                     LSLRKGTTLTKEMFLNHLYGGMDEPELKIIDVFICKLRKKLDAASGGQNYIETVWGRG
                     YVLREPEDVRESA"
BASE COUNT         1579 a         2295 c         2478 g         1561 t
ORIGIN      
        1 cgacagatag gcgtcgacga ccgtggtgtc gccacggccg atcagcttga ccagcggcga
       61 cgcttcgtgg ctgacggata cctggccgaa gccgagcttg cggcagagtt tcgcgacagc
      121 ctgctcgtgg ccgggatatt tccaggcatg catgaagacg atggcgacgg catcaatgcc
      181 atcggccttg gcctgcttga tcgccggcag gacatcggca atgtcgagca gcctttcgat
      241 gcagccatcg gcacggatac gctcatcgac ctcgacgacg cgctcgtaaa gccgttcggg
      301 caggatgatt tccttggcga aaatatcggg ccgcgcctgg taggcgatgg ccagcgcatc
      361 gcgaaacccc ttggttataa gcagcagcac gcggtcgccc ttgcgttcca gcaacgcatt
      421 ggtggccact gtcgtaccca tgcggatatc gccgatcagg tcgacgggaa caggctcgcc
      481 cggttccagc ccaagcaggc cacgaatgcc ggcgatggcg gcgtcggcat aggcctccgg
      541 attttcggac aggagcttgc gcgggtgcag gccgccatcg ggatcgcgcc cgataatatc
      601 ggtgaaggta ccgccacggt cgatccagaa attccacttc gccgtcattg ccgttccgct
      661 ttgctgaccc cctttgagtc ggggcgtcgg cactgtggtc ggggcggcgg gacgcggcaa
      721 gaaaaaacgg ctgcggcggc cggcggagcg agcctggacg gcagggaatg cgccgcaaag
      781 atgacgctca aacggaagct tgccggacag ggccatgctt tgtttgactt gtcgcggccc
      841 aggccggaaa ttcgcgcttg cgggtcatcc ggctcaggcg cggccgatat gcgcaagctt
      901 cggatcattt aatgcttgga ggacgccagc cgtgaaaatc accgatgtaa agaccttcgt
      961 cgtcggcaac ccgccgcccg gcatcggcgg caaatatttc atcttcgtca agctgacgac
     1021 ggatggcggc gtggtcggct atggcgaggc ctataacgcc acgttttccg cccatatcac
     1081 ggcgcggatg atcgaggaca tggccgagcg ttatctggtc ggccacgatc cgcacgacat
     1141 cgaaacgctg tttcgccgct gttattcctc cggctttacc cagcgcccgg acgtgtcggc
     1201 catgggctgt ttttcggcgc tcgaaatcgc ctgctgggac attatcggca aggaagcggg
     1261 caagccgatc tacaagctgc tgggcgggca ggtgcatgag aagctgcgaa gctacaccta
     1321 cctctatccc catagcggca gcgttcacac cgaggacgtg gacggcaaga acgtctataa
     1381 tgatcccgat ctcgccgccg aatgcgccgc gctttatgtc gagcagggtt tcaacgcagt
     1441 gaagctcgat ccggccgggc cttacacggc cttcgacggc caccagccgc gcctggcgga
     1501 catcgacctt tccgcgcgca tgatgaaggc gatccgcgag gcggtcggca accgggccga
     1561 catcttgttt gggacgcatg gccagttcac ggcatcgggc gcgctgcgca tggcggcggc
     1621 gatcgaacct tacgatccgc tatggttcga ggagccggtg ccgccggaca tgcccgaggt
     1681 gatggcgcag gtggcgcggg gcacccgcat tcctatcgcc actggcgagc ggctgacgac
     1741 caagttcgaa ttcgcgcgcg tcattgaaaa ccgcgccgcg gccatcctcc agccggacct
     1801 tggccgctcg ggcggtatcc tggagacgaa gaagatcgcc gcgatggcgg aagcctacca
     1861 catccagatc gcgccgcatt gctattgcgg cccgattgtc ggcgcggcca atgtccaggt
     1921 ggcggcgacg ctgccgaatt tcctcatcct ggaatccttg aagcaatggg atagcttcca
     1981 cgccaagctg ctcaagcaga aaatcgaatg gcaggatggc aacgtcattc cttcaaagga
     2041 acccggcctc ggcgtcgaac tggatgaggc cgtttgcgag gcgcatcctt acgagggcaa
     2101 ggcgctgcac cttgaaatga agctcgatcc gttgtttccc taagcggctc ggcaaagggt
     2161 tttagccgtt cgttcagtgg ggtggtgacc gcccagttga ccaattttgg tagcgaaaac
     2221 ccgaattttc aaagagaata cagcaccgcg cgtccaaagg gcggcagcgc ggcccatctg
     2281 aaccggttct cgtcaaaatg aaaatcccgg ccggcaagcc gggattttca aaaggccgga
     2341 tcagccccaa acccagacgc gacggcgata gcagcggaca tgccgccggc cgcgacgcca
     2401 gtagctactg caaaccctgc gccagacacg tcgccgatgc cggcggttat ggcggcgatt
     2461 atcgcggcgc caatgccggt cccgtcggta ctggacgggt tccgcctcgg tggcgggttc
     2521 atcaagctca tcgagaatgc cgtcgctgcc gggcatcacg cccgccatcg tgctgtccgg
     2581 ctgggctacg cctatgagcg cggccgcgcc ggcaaggccg agcatgccag tcagaaaaga
     2641 tcgtcgatcc atgaatacct ccatcagttg gcgatggaga aaatagcgcg gcgaacttca
     2701 acgccaagtc cggggatgat tttcgcgttc gccaagcttg ccggatatgc gaggcgcacc
     2761 cgcaagatgc cggctccctg cgagccaagc cggtgtcaaa gccttgattt ctttaaggaa
     2821 agaatggtag cgggaagtgg actcgaacca ccgaccccag gattatgatt cccgtgctct
     2881 aaccaactga gctatcccgc cagggcgacg aaaattcgag atgcattgtg catgccggga
     2941 ctttcgccaa ggtcgggcgg gtataatgtt gcaagggttg gcatgtcaag cttggaagcg
     3001 ggtgcgttgc cagcataatt cccggccggc gagaactggg gcgcagacat ttcagcgcag
     3061 ctgccggggt tgagttcaga gggccgcacc gctatgtgtc agccaagtaa ttttcgagtt
     3121 cgctattgat gataccacgc attgcagtcc tcgggtgcgg atactggggc agcaaccaca
     3181 tccgcacctt gaaggcgctt ggcgcattat acgcggtttc ggatgcgaat ccggcgcgcg
     3241 ccgaaggttt tgccagcgaa caggattgcc tggcaatcgc cccggatgcg ctgtttgcaa
     3301 acccggacat cgatgccgtc gtcctggcgc tgccgccgca atttcatgcc gacatggcgg
     3361 tgcgcgcggt cgagaacggc aaggacgttc tggtagaaaa gcctatcgcg ctgaccgttc
     3421 ccgatgccga acgcgcggtg aaggcggcgc gcgacaatgg ccgcgtcttc atggtcggcc
     3481 atgtgctgcg tttccatccg gctttcgaaa cgctgaaggc gctgatcgac aagggcgaac
     3541 ttggcgaggt gcgctatatc cattcgcatc gcctcgggct tggaaaattc cataccgaaa
     3601 acgatgcgct ctgggatctg gcgccgcacg atctgtcgat gatcctggcc attaccggca
     3661 ccgagccgct ggaagtgcat ggcgagggtg cggcgatcct cgataatctc agcgacttcg
     3721 cccatctgca catgcgtttt ccgcatggcc tctccggcca tctctttgcc tcccggctca
     3781 atccctacag ggagcgtcgg ttgacggtcg ttggcaccaa ggcgatggcc gtgttcgacg
     3841 atgtcgagcc gtgggagcgc aagcttgccg tctatcgcca cgcggtctgg caagacagcg
     3901 gccagtgggc gttcacgacc aacgagccga cctatgtggc ggtcaagcag ggcatgccgc
     3961 tgacgcgcga actggaacat ttcatccagt gcatccagac gcgcgccgag ccgcgcaccg
     4021 atggccagga agcgatcact gtgctgaagg tgctgaccgc aggcaccgtc aaacacgaca
     4081 agcgccgccg ctagcaacgg gcaataatcg ccggtagttg tgggcggcaa ggccggcagg
     4141 cgcgctgcct gtcggtattt gcctattctg ctgcgatatt ggccgggttg gccgccaggc
     4201 ggtccagcat ggcttcggca tcggcgctgc gctcggagcg ggcgatgaag ccgccgccga
     4261 atacgcgcgc ctcgttgccg tcgtcggaat aaagcacgca ggcctggccg ggcgcgatgc
     4321 cggattcgcc atccgacaat tctacccaga tcgcaccatc gcgatgccgc aaaacagcgg
     4381 ggcgcggcgg ccttgtggat cgcaccttgg cgaaaagctc cagcccgtca tgcgcaatgt
     4441 cggacaagtc gccgtcaccc agccagttca tgtcgcgcag gtaaatcttg tgcgtttcca
     4501 gcgcttcacg cgggccgacg acgacgcggg cgttgtcggc atccaggtgt acgacataaa
     4561 gcggctcgcc ggatgcgatg ccgatgccgc ggcgctggcc gatcgtataa cgcaagatac
     4621 cttcgtgatg gccgagcacg cgtccgtcta tatggacgat gttgccggga atggtcgccg
     4681 tcgggcgcaa cttggcgatg acgtcggaat atttcccctg cggcacgaag catatgtcct
     4741 ggctgtcatg cttggcggcg acggacaggc ccatctcctc tgcgatggcg cgcacctgct
     4801 gcttgggtag atggccgagc ggaaaacgca aatagtcgat ctgcgcctgc gtggtggcga
     4861 acaggaaata gctctggtcg cgatcggcat cgacgggccg gtagagcgag cggtgcgcgc
     4921 cgttggcgcg ggagcgaatg taatggccag tcgccagtgc atccgcctcc aactcctgcg
     4981 cggtggtaag aaggtcggca aactttacgg tctggttgca ggccacgcag gggataggcg
     5041 tttcgccggc catatagctg gtggcaaacg ggtcgatcac cgccttgcgg aaacgttcct
     5101 cgtaattcag cacatagtgc gggatgccca gcgtttcggc gacgcgccgc gcatcgtcaa
     5161 tgtcctggcc ggcgcagcac gaaccggcgc gatgggtggc ggcgccatga tcgtaaagct
     5221 gaagcgtcac gccgacgaca tcatagcctt catgcttgag cagcccggcc acgacggagg
     5281 aatcgacgcc tcccgacatg gccacgacga tgcgggtgtc ttcggggcga gcgggcagat
     5341 caaggctgtt catggtcagg tccgtattgt caacgcttcg ggttcaaggc ccgaacgatg
     5401 ccgtgaatat aggccaagcc ggacgtttgc gccagcaccg gcaccgaatg ccctgatgcg
     5461 acatggtggg ggcgggcctg caacgaggtt tgtgcgggtt ggttcaaatg ccgtttaaaa
     5521 ccctaacgcg ccgttcatga tccttaccta tccattaggg taggcttagg caaattttaa
     5581 agctgtggcg gtaatgtggt ggggattggg tctttgagtt tttagtagag agtacgatga
     5641 ccgatctaat tagaccgcga gtcaaatatg ttatcgggcc tgacggcagc cctcttacca
     5701 ttgccgatct gccgccgacc aacacgcgtc gctgggttat ccggcgtaag gcggaagtcg
     5761 tcgccgccgt cagaggcggg ttgctcagtc ttgaggaggc gtgccaacgc tacaagctga
     5821 cgacggaaga gtttttgtcg tggcaggcct ccatcgacga gtatgggctg gccgggttgc
     5881 gaacgacccg tatccagcag taccggcatt aatcgcaaag cgcccggccg atgggcaggg
     5941 cgttttttct cccctgggaa tggggccgcg cgggcgcggc ccgaattgct attatcctgc
     6001 ggtggttgga tggggcgggt ctggacgctt ggagcgctgt cgcccgtgcg ggccggcttg
     6061 ttttcgggat cgagggaaat cgcgctgccc ggcgcttgag cgtggcaggc gcgtagcctc
     6121 agccttttcc gtagcctatt ctggcgcggc ttagccggac aatatttagc gggaaaagaa
     6181 gccgttcgcc ggcctggctt gtcgctggaa gcggaggctt gctcgcttga agcggcggct
     6241 gcctgctacg ggaatcgagc ggcaggataa atttcgccct tagcggcgcg ggccaatgcc
     6301 gcgctcttga cgccgcacca gagtgcgacc cctgccgttt tagccgatca tggcgcttcg
     6361 ggcgaccatc tcgacgtcgc gaaccttgat gtcgcgtgat tccagcattt ccgccttgga
     6421 aagctccgct tcagcttcgg cgagtattga ttcggcagcg cttcgctgtt gcgcgagatc
     6481 ggactgcgaa ttgcggagat tgtcgcggcg caggcgcgct gcggtggcaa aggttgggta
     6541 ggcgaaatga ttgatatcgg tgatgccggc cttcttttcc tcggcggcga tctgcaattc
     6601 cagttcggtg gccatccgct cgaattcggc gatcatcatg tcgagctgca acagctgccg
     6661 ccgcttctca ttcacctgaa actgtttcag tcgaaccagg ttttcacgtg acttcatgat
     6721 tcggtactcc agcaaacgca ctcatgcaca tagcgtccaa atgaccctga aaggtcgtgt
     6781 cccctacatt tcccccggct ttccccgagc tatgggaaac aaaatcctaa agttttttcg
     6841 tgccggtaac cattcgttta cgggcattgt taatcatagg ggcgaggact taagggctgg
     6901 taaaaatttc aggcgcccaa aaatgccgga cgatgaaggt cccaatgagt ctaaagagtc
     6961 acttagccag gttttttaat gtgcggagct agtgttttag ggcaacgatt ccgcattgga
     7021 ttcaagtgag tgtagaaatt ggttaaaaaa tctggtatcg cgttgggtgc gcaattagga
     7081 tttgttaacc attaagtggc agctttcgac aaggcaatcg ctgctccggt cccggacggc
     7141 tcagatacgg accgatagca gaaaagggga atgagatgcg cgttctgctg atagaagacg
     7201 acagtgcaac cgcacagagc atcgaattga tgctgaagtc tgaaagcttc aatgtgtata
     7261 cgacagacct tggcgaagaa ggtgtcgatc tcggcaagct gtatgactac gacattattc
     7321 ttctcgatct gaatctcccc gacatgtcgg gttatgaggt cttgagaacg cttcgcctct
     7381 ccaaggtcaa aacgccgatc cttattctct ccggcatggc cggcatcgag gacaaggtgc
     7441 gcggcctcgg cttcggcgcc gacgactata tgaccaagcc gttccacaag gacgagctgg
     7501 tcgcccgcat ccacgccatc gtgcgccgtt ccaagggcca tgcccagtcg gtcatcacca
     7561 cgggcgaact ggtggttaat ctggatgcca agacggtcga ggtcagcacc cagcgcgtgc
     7621 atctcaccgg caaggaatac cagatgctgg agctgctttc gctgcgcaag ggcacgacgc
     7681 ttaccaagga aatgttcctc aaccacctct atggcgggat ggacgagccg gaactgaaga
     7741 tcatcgacgt tttcatctgc aagctgcgca agaaactcga cgctgcttcg ggcggccaga
     7801 attacatcga aacggtgtgg ggccgcggct atgtgctgcg cgagccagaa gatgtccgcg
     7861 aaagcgcctg aaagttcgaa attccgttac aaaaaagtcc ggctcagccg gac
//
LOCUS       DASYCS010000290         1587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4132788,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000290 DASYCS010000000
VERSION     DASYCS010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4132788"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="VGM46_09180"
     CDS             complement(<1..562)
                     /locus_tag="VGM46_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011662269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY4192803.1"
                     /translation="MPTIALVDDDRNILTSVSIALEAEGYRIMTYTDGASALDGFRTS
                     QPDLAILDIKMPRMDGMETLRRLRQKSDLPVIFLTSKDEEIDELFGLKMGADDFIRKP
                     FSQRLLVERVKAVLRRAAPKDPTAVPKETDAKALDRGLLRMDPERHTCTWKNEPVTLT
                     VTEFLILQALATRPGVVKSRNALMDAA"
     gene            778..1512
                     /locus_tag="VGM46_09185"
     CDS             778..1512
                     /locus_tag="VGM46_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2470 domain-containing protein"
                     /protein_id="HEY4192804.1"
                     /translation="MEPSADFDPSRLARSLLRRSRQGALATLMAGSGDPYCSLVNVAS
                     HADGSPILLISRLALHTRNILGDARVSLMLDERAAGDPLEGARIMLAGRAEQAGADQV
                     AVLRRRYLNAHPSAEAFVEFKDFSFFRIHPTAAHLVAGFGRIVDLPPERFLTDISDAT
                     ALLEAEPGAIEHMNEDHCDAMNLYATKLLGAEAADWRCIGCDPEGMDMQAGAATLRLE
                     FPERVTNGNELRKMLVRLVGEARAKA"
BASE COUNT          301 a          472 c          505 g          309 t
ORIGIN      
        1 aggctgcatc catcagcgca ttgcggctct ttaccacgcc ggggcgcgtc gccagtgcct
       61 gcaggatgag gaattccgtc accgtcaacg tgaccggttc gtttttccat gtgcaggtgt
      121 gacgctcggg atccatgcgc agcaagccgc gatcgagcgc cttggcgtcg gtctccttgg
      181 ggaccgcggt cggatccttt ggcgcggcgc ggcgcaacac cgccttgacg cgctcgacca
      241 gcaggcgctg cgagaacggc ttgcggatga aatcgtcagc gcccattttg aggccgaaca
      301 attcatcgat ctcctcgtcc ttggaggtga gaaagatcac cggcagatcc gatttctgcc
      361 gcagccggcg caacgtctcc atgccgtcca tgcgcggcat cttgatatct aggatggcaa
      421 ggtctggctg gctggtgcga aagccatcga gcgccgacgc gccatccgta taggtcatga
      481 tacggtagcc ttcggcttcc agcgcgatcg agaccgatgt gaggatgttg cggtcgtcat
      541 cgaccaaggc gattgtgggc atgagcctgc tttcgaaaaa aacggtggct gaaacggcga
      601 gctatttagc gatgcgccgt acaagtgggc ttcgaacgcg gtcaacgagc aatgcaagct
      661 aggctgaagt gtgaccgagt tccgaaaacg cctcgcgcgg caaagcgttc caacccgtgg
      721 taatcctatt tacccgaaaa aaggccttca ttgcaacagg ataccccgga ttaaagcatg
      781 gaaccatcag ctgatttcga tccttccagg ttggcccggt cgctgctgcg gcgcagccgg
      841 cagggggcgc tggcgacctt gatggccggc agcggcgatc cctattgttc cctggtgaat
      901 gtcgcgagcc acgccgatgg ctcaccgatt ctgctgattt cgcggctggc gttgcacacc
      961 agaaacatcc ttggcgatgc cagggtttcc ctgatgctgg acgagcgggc cgccggcgac
     1021 ccgctcgagg gcgcccggat catgctggcg ggccgcgcgg aacaggccgg ggccgaccag
     1081 gtcgcagtgc tgcgccggcg ctaccttaac gcccatccgt cggcggaagc ctttgtcgaa
     1141 tttaaggatt tttccttctt ccggatccat cccacagccg cccatctggt ggccggattc
     1201 ggccggatcg tggatctgcc gccggaacgg ttcttgaccg acatttccga tgccacggcg
     1261 ctgctggaag ccgagccggg cgccatcgaa catatgaacg aagatcactg cgacgccatg
     1321 aatctgtacg cgaccaaatt gctgggcgcg gaagccgccg actggcgctg catcggctgc
     1381 gatcccgagg ggatggacat gcaggctggg gcggcgacgc tgcggctgga gttcccggag
     1441 cgcgtcacca acggcaacga actgcgcaag atgctggtga ggttggtggg tgaggcccgc
     1501 gccaaggctt aaacttttcg aaaggctcaa atttttcgat cctgagactc atcgcgccgg
     1561 aacattgaac gggcgcgtgg gttcacg
//
LOCUS       DASYCS010000291         6783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4569879,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000291 DASYCS010000000
VERSION     DASYCS010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 6783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6783
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4569879"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="VGM46_09190"
     CDS             <1..901
                     /locus_tag="VGM46_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015317759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEY4192805.1"
                     /translation="GIKPTYGRCSRWGIVAFASSLDQAGPIARDVRDAAILLKSMASV
                     DAKDTTSVDLPVPDYEAAIGKPVKGMKVGIPKEYRVDGMPQEIEALWQKGIAWLKDAG
                     AEIVDISLPHTKYALPAYYIVAPAEASSNLARYDGVRYGLRVPGKDIVEMYENTRAAG
                     FGQEVKRRIMIGTYVLSAGYYDAYYIKAQQVRTLIKKDFEDAFAAGIDVILTPATPSA
                     AFGVADQEMASDPVKMYLNDIFTVTVNMAGLPGISVPAGLDGNGLPLGLQLIGKPFEE
                     ETLFQTAHVIEQAAGTFQPEKWW"
     gene            927..1721
                     /locus_tag="VGM46_09195"
     CDS             927..1721
                     /locus_tag="VGM46_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEY4192806.1"
                     /translation="MFFILSKIVGFVIQPVNFAIILIFIGLAATFFRWRRLGAACSGG
                     AFLVLALSVWTSLGAWMLNPLEQRFPKPDFPQKVDGIVVLGGGLEGGINLARGGYELN
                     SAGDRLVEAAVLARRYAKAKIVITGGNGSLMLAGEGDAGTAPRLLEALGVAPDRLILE
                     NRSRNTYENAVFTREVVTPKPGETWLLVTSAFHMPRAKALFDKAGFATVPWPVDYRTS
                     GREGIGLFTDNAADALQATTIALREWIGLVAYWLSGKIDTPFPGPA"
     gene            1860..3263
                     /locus_tag="VGM46_09200"
     CDS             1860..3263
                     /locus_tag="VGM46_09200"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012091942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HEY4192807.1"
                     /translation="MFLSVFDLFKIGIGPSSSHTMGPMTAAARFLEEILNGDWPRPAG
                     VKVDRLGASLHGSLAYTGIGHGTDRAVILGLTGETPMTIDPDRADGVIAAITADKRIS
                     PPGHPSYRFDPATDLVMDRKTKLAGHANGMAFYAYDAADRLLLKRIYYSVGGGFVMSE
                     EELQRAKTSGSVTTEGKKVPYPFKRADEMLQMASKSGLSIAEMKRVNEETQMSREKLD
                     EGLDAIWNAMLNCIERGLSQEGIMPGGLKVRRRARMLHDKLQEQWSQNRPNPLLANDW
                     LSVYAMAVNEENAAGGRVVTAPTNGAAGTLPAVLRYWLHFHPEADQPSIRDFLLTAAA
                     VGGIIKTNASISGAEVGCQGEVGSASAMAAAGLCAVMGGTPEQVENAAEIALEHHLGM
                     TCDPVGGLVQVPCIERNALGAVKAVTAASLAIKGDGTHFVPLDAAIETMRQTGRDMNE
                     KYKETSLGGLAVNVVEC"
     gene            3370..3807
                     /locus_tag="VGM46_09205"
     CDS             3370..3807
                     /locus_tag="VGM46_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1489 family protein"
                     /protein_id="HEY4192808.1"
                     /translation="MPLNILKLCVGCDSVEDLEEWVASCLDERRRNGKPVEHWHTTRM
                     TPTRGAEIVGAGSLYWVIKGNVQCRQLITEIRPFVDGEGIGRCMLMLDPQIVRTDWQP
                     RRAFQGWRYLKQADAPVDLGKGNAAMAEMPPKLRLELAELGLL"
     gene            3962..4657
                     /locus_tag="VGM46_09210"
     CDS             3962..4657
                     /locus_tag="VGM46_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006334635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEY4192809.1"
                     /translation="MGVLIAIVAVFLAIVGLVTVFLRADPAKLAGGLTLTAPLLLAFI
                     GLAALIAGREAIGGLMLMGAFAWYGSMRKKPAPRLTAARHATVRTAALEMELDHGTGA
                     LEGLVLAGRHEGRMLGSMGLAELRELYRDLYTDPESRQLLETYLDGRFPVWRKHAQPD
                     GGERLGVPPGSGPMTKEEAYKVLGLEAGAAPTDVRKAHRRLMQRLRPDIGGTSFLAAR
                     INEAKDVLLSNHH"
     gene            complement(4701..6113)
                     /locus_tag="VGM46_09215"
     CDS             complement(4701..6113)
                     /locus_tag="VGM46_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008875927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEY4192810.1"
                     /translation="MRQALSGIISRSANLSKAVMMLAAAVTFIAAQTASSMATPHSAI
                     IIDANTGKTLYASDANGRRYPASLTKMMTLYLTFEALSSGKISRSSKVTFSANAAAEP
                     PTKLGVKKGGQVTVDAAILSMVTKSANDSATAMGEFLGGNEANFARMMTAKARALGMR
                     STVFRNANGLPNPGQFTTAHDMAILGIALREHFPQYYGYFSQRSFMFGRQRINGHNRV
                     LDRVKGADGIKTGYTAASGFNLVTSVKDGGRNIVGVVMGGSSGGSRDSEMVAMLKTYM
                     PKASSRGGSGDLIAKADDDDTPVVALAKAILPKHNAPTPDIRPAREELASAPDNVEPL
                     QEDIPFKTADESAPAAKTPAPIIAKAARPITASVVAAAYAEPEENVDPVNTASLPSGW
                     AVQVASSPSKGEAQAILEKTSKQASRILADASGFTAPFKKDGVTYFRARYSGFGSKDA
                     AWKACNGLKKHKIQCYAVQQ"
     gene            6415..6744
                     /locus_tag="VGM46_09220"
     CDS             6415..6744
                     /locus_tag="VGM46_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEY4192811.1"
                     /translation="MTQSLEEFSRYGRSLAESGMNSLDSLSKGAQAVAAEAGEYTGKT
                     LEEGSAAAAKLFAAKSLEDALEIQSAYLRHSYESLVAGATRFGNLYAELAGEACKPFE
                     GVVARVG"
BASE COUNT         1242 a         2093 c         2166 g         1282 t
ORIGIN      
        1 cggcatcaag ccgacctatg gccgctgctc gcgctggggc atcgttgcct tcgcctcgtc
       61 gctcgatcag gccggcccga tcgcgcgcga cgtgcgcgac gccgcgatcc ttttgaaatc
      121 catggcctcc gtggacgcca aagacacgac ttccgttgac ctgccggtgc cggattacga
      181 ggccgccatc ggcaagccgg tcaagggcat gaaggtcggc atcccgaagg aataccgcgt
      241 cgacggcatg ccgcaggaga tcgaggcgct gtggcagaag ggcattgcct ggctgaagga
      301 tgcgggcgcg gaaatcgtcg atatctcgct gccgcacacc aaatacgcgc tgccggccta
      361 ttacatcgtc gcgccggccg aggcctcatc caacctcgcc cgctatgacg gcgtccgcta
      421 cggcctgcgc gtgccgggca aggacattgt agaaatgtac gaaaacaccc gcgccgccgg
      481 cttcggccag gaggtcaagc gccgcatcat gatcggcacc tatgtgctgt cggccggtta
      541 ttacgacgcc tattacatta aggcgcagca ggtgcgcacg ctgatcaaga aggatttcga
      601 ggacgccttc gccgctggca tcgacgtcat cctgacgccg gcgacaccgt cggccgcctt
      661 cggcgtcgcc gatcaggaaa tggcctccga tccggtcaag atgtatctca acgacatttt
      721 cacggtgacg gtgaacatgg ccggcctgcc cggcatttca gtgccagccg gcctcgacgg
      781 caacggcctg ccgcttggat tgcagctgat cggcaagccg ttcgaggaag agacgctgtt
      841 ccagaccgcg catgtcatcg aacaggccgc cggcacgttc cagccggaaa agtggtggta
      901 atactatgct agggagggga tgacgaatgt tcttcatcct ctccaagatc gtcggcttcg
      961 tcatccagcc ggttaatttc gcgattattc tgatttttat cggccttgcg gcgacgttct
     1021 ttcgctggcg gcggctgggg gcggcctgta gcggcggcgc gtttctcgtc ctcgcgcttt
     1081 ccgtgtggac ttcgcttggc gcgtggatgc tcaacccact ggagcagcgc ttccccaagc
     1141 ccgattttcc gcaaaaggtg gacggcatcg tcgttctcgg cggtggcctt gaaggcggca
     1201 tcaaccttgc gcgcggcggc tatgaactga attcggccgg cgatcggctg gtcgaggcgg
     1261 cggtgctggc gcgccgctat gccaaggcga agatcgtcat caccggcggc aacggctcgc
     1321 ttatgctggc gggcgagggc gatgccggca ccgctccccg cctgctggaa gcgctgggcg
     1381 tcgcgccgga tcgcctgatc ctcgaaaacc gctcgcgcaa cacctacgaa aatgccgtct
     1441 ttaccaggga ggtggtaacg cccaaacccg gcgagacatg gctgctggta acgtcggcct
     1501 tccacatgcc gcgcgccaag gccttgttcg acaaggccgg cttcgccacc gttccatggc
     1561 cggtcgacta tcgcacctcc ggcagagagg gcatcggcct gtttaccgac aacgccgccg
     1621 atgccttgca ggcgacaacg attgccttgc gcgaatggat cggccttgtc gcctactggc
     1681 tgtccggcaa gatcgatacg ccatttccgg ggccagcctg aacattccgc ccgcaatgtc
     1741 gcggggggat catccaaggt cggcgcgtgc cgcgcgactt tgcggaaaac agcaaataag
     1801 gtgagagttc ctatatatct gtcgctatgc tcgacatgag atagcctggg agatttcgcg
     1861 tgttcctttc ggtttttgac ctgttcaaga tcggcatcgg gccatcgagt tcccatacga
     1921 tgggaccgat gactgcggcg gcgcgctttc tggaggaaat cctgaacggc gactggccgc
     1981 gccccgccgg cgtgaaagtc gatcgcctgg gcgccagcct gcacggctcg ctggcctata
     2041 ccggcatcgg ccatggcacc gatcgcgccg tcatccttgg cctgaccggc gaaacgccga
     2101 tgaccatcga ccccgacagg gcggatggcg tcatcgcggc gatcaccgca gacaagcgca
     2161 tttcgccgcc cggccacccg tcctaccgct ttgatccggc caccgatctg gtcatggacc
     2221 ggaagaccaa gttggccggc cacgccaacg gcatggcttt ctacgcctat gatgcggctg
     2281 atcgcctgct gctcaagcgc atctattatt ccgtcggcgg cggcttcgtc atgtcggagg
     2341 aagaacttca gcgcgccaag acatccggtt ccgtcaccac cgaaggcaaa aaggtgccat
     2401 accccttcaa gcgcgccgac gaaatgctgc aaatggcgtc gaaaagcggc ctttccatcg
     2461 ccgagatgaa gcgggtcaac gaagaaacgc agatgtcgcg cgaaaagctc gacgagggcc
     2521 tggatgcgat ctggaacgcg atgctcaact gcatcgagcg cggcctgtcg caggaaggca
     2581 tcatgcccgg cggcctgaaa gtgcgcaggc gagcccgcat gctgcacgac aaattgcagg
     2641 agcaatggag ccagaacagg ccgaacccgc tgctcgccaa cgattggctg tcggtctacg
     2701 ccatggccgt caacgaggag aacgccgccg gcggccgcgt tgtcaccgca ccgaccaacg
     2761 gcgcagccgg cacgctgccc gccgtcctgc gctactggct gcatttccac ccggaagccg
     2821 accagccgag catccgcgac ttcctgctga cggccgccgc cgttggcggc atcatcaaga
     2881 ccaacgcctc catttccggt gctgaggtcg gttgccaggg cgaggtcggt tccgcttccg
     2941 ccatggcggc ggctggcctg tgcgcggtca tgggcggtac gccggagcag gtggaaaatg
     3001 ccgccgaaat cgcgctggaa caccatctcg gcatgacctg cgatcctgtc ggcggtctgg
     3061 tccaggtgcc gtgcatcgag cgcaacgcgc ttggcgccgt caaggcggtg acggcggcgt
     3121 cgctcgccat caagggtgat ggcacccatt tcgtgccgct cgacgcggca atcgaaacca
     3181 tgcgccagac cggccgcgac atgaatgaga aatacaagga aaccagcctt ggcggcctcg
     3241 ccgtcaatgt ggtggaatgt tgaacgcctt ttcggaaaaa ggccggctgt aaacgggata
     3301 taacgcgaga gcctgtgcaa aacgcagccc ggcgttgaat ggtccgggcg caggcactaa
     3361 gttgcgctca tgcctctgaa tatcctgaaa ttatgtgtcg gctgcgacag cgtcgaggac
     3421 ctcgaagaat gggtcgcctc ctgcctggac gagcgccgcc gcaacggcaa gccggtcgag
     3481 cactggcaca ccacccgcat gacgccgacc cgaggcgctg aaatcgtcgg cgccggctcg
     3541 ctctactggg tcatcaaagg caatgttcag tgccgccagc tgattaccga aatccgtccg
     3601 ttcgtcgatg gcgagggcat cggccgctgc atgctgatgc tcgatccgca gatcgtgcgc
     3661 accgactggc agccgcgccg cgcctttcag ggctggcgct atctgaaaca ggccgacgcg
     3721 ccggttgatc tcggcaaggg caacgcagcc atggcggaaa tgccgccgaa actgcggctg
     3781 gaactggctg aactcggcct gctgtagtcg ccggcctggg tggccatatc gcccggccaa
     3841 tggcgacacg ggcttgccgg ccacatattc gcgccacatc ctggatcgat gttgggcgtt
     3901 gggtgaaatc ggctgtggcc aaggcggtct cggctgaagt cctgcgaggt gaccggtgct
     3961 gatgggcgtg cttattgcga ttgtcgccgt gtttttagcc atagtcgggc tagtgacggt
     4021 cttcctgcgc gccgatccgg caaaactggc cggcgggctg acgctgaccg caccgctgct
     4081 gctggcattt atcggcttgg cggcattgat tgccggacgc gaggcgatag gcgggctgat
     4141 gctgatgggc gccttcgcct ggtacgggtc gatgcggaag aagcccgcgc cccgattgac
     4201 ggcggcaaga catgccaccg ttcgcacggc cgcgctcgaa atggagctgg accacggaac
     4261 cggcgcattg gaagggctgg tgctcgccgg ccgccatgaa ggcaggatgc tgggttcgat
     4321 gggcctggca gaattgcggg agctataccg cgatctctat accgatcccg aaagccggca
     4381 attgctagag acttatcttg acggccgctt tcccgtctgg cgcaaacacg cgcagccgga
     4441 cggtggcgag cggctgggtg tgccgccagg ttctggcccc atgactaagg aggaggccta
     4501 caaggtcctt ggtcttgaag cgggagccgc tccgacggat gtccgcaagg cgcaccgccg
     4561 cctgatgcag cgcctgcgcc ccgatatcgg gggcacgtct ttcctggcgg cgcggatcaa
     4621 cgaagccaag gacgtcttgc tctccaatca ccactaaatc tccttcgacc agaaacattc
     4681 cggagagagt tccatgccgc ctattgctgg acggcataac actggatttt atgcttcttc
     4741 aggccgttgc aggccttcca ggcagcgtct ttcgagccga acccgctata gcgggcgcgg
     4801 aaataggtga cgccgtcctt cttgaaggga gcggtgaagc ccgaggcgtc ggcaagaatt
     4861 cgcgatgcct gcttggaggt cttttccagg atcgcttgcg cctcgccctt gctgggcgaa
     4921 gaggcgacct gcacggccca gccggatggc agcgatgccg tgttcaccgg atcaacgttt
     4981 tcctccggct cggcataggc ggctgcaacg accgaagcgg taatcggcct ggctgccttg
     5041 gcgatgattg gcgcgggggt ctttgccgcc ggcgcgcttt catccgccgt cttgaacgga
     5101 atgtcttcct gcaaaggctc tacattgtcg ggagcggagg caagttcctc acgcgccggg
     5161 cggatatccg gggtcggcgc gttatgtttg ggcaaaatcg cttttgccag cgccacgacg
     5221 ggcgtgtcgt cgtcatcggc cttggcgatc aggtcgccgc tgccgccgcg cgacgatgcc
     5281 ttcggcatgt aagtcttgag catcgcgacc atttcgctgt cgcggctgcc gccggagctg
     5341 ccgcccatga cgacgccgac gatgttgcgg ccgccatcct tcacggaggt gaccagattg
     5401 aagccggaag ccgccgtata gccggtcttg atgccgtcag cgcccttgac gcggtccagc
     5461 acccggttgt ggccattgat gcgctgcctg ccgaacatga acgaacgctg cgagaaatag
     5521 ccgtaatatt gcgggaagtg ctcgcgcagg gcgatgccga ggatagccat gtcgtgcgcg
     5581 gtggtgaact ggccgggatt gggcaggccg ttggcattgc ggaacacggt cgacctcatg
     5641 cccagcgcgc gcgccttcgc ggtcatcatg cgggcgaaat tggcctcgtt tccacccagg
     5701 aattcgccca tggcggtcgc ggaatcgtta gccgatttcg ttaccatcga caggatcgca
     5761 gcgtcgacgg tcacctggcc gcccttcttc acgccaagct tggtcggcgg ctcggcggcg
     5821 gcgttggctg agaatgtgac tttggacgac cggctgatct tgccgctcga aagcgcttcg
     5881 aacgtcaaat aaagcgtcat catcttggtc agcgatgccg ggtagcgccg gccattggcg
     5941 tcggatgcgt agagcgtctt gccggtattg gcgtcgatga tgatggccga gtgcggcgtg
     6001 gccatcgacg aggccgtctg agcggcgatg aatgtcaccg ccgcggcgag catcatgacc
     6061 gctttggaaa gattggctga cctggagatg atgcctgaca acgcctgacg cactgctaca
     6121 tcctttgaat tttgttgcat ggggaggcgg caaaggttcc agcctcgcat tcggcgcaaa
     6181 ctacgtcggc agcgttacca atccgtttat ggtaaccgcg ccattcacca ttttcgtcgg
     6241 agttgactca cattttacgt gaattgggtg ggcgttcggc gcgtctgcgg ctgcacgccg
     6301 cagtcgaaat tgacttcttg tgcgtcgcac catagatttt gccgcagcgc accagctgcg
     6361 ctggccgata ttcgagcctg aaaaccaata ttctcgcctg aaaaggatgt cgccatgacg
     6421 cagtcgctcg aagaattcag ccgctacggc aggagtttgg cggaatccgg catgaacagc
     6481 ctggattcgc tttcgaaagg cgcgcaggcc gtcgcggccg aagccggcga atataccggc
     6541 aagacactgg aagaaggcag cgccgctgcc gcaaagctgt ttgcggcgaa gtcgctggag
     6601 gatgcgctgg aaatccagtc cgcctacctc aggcattcct acgagagcct ggtcgcgggg
     6661 gcgacccggt ttggcaacct ttacgccgaa ctggccggcg aggcctgcaa gccgttcgag
     6721 ggcgtcgtcg ccagggtcgg ataagcttcg gtcactatcc tgccgaggca ggtcggtcag
     6781 gca
//
LOCUS       DASYCS010000292         8069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4856335,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000292 DASYCS010000000
VERSION     DASYCS010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 8069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8069
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4856335"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="VGM46_09225"
     CDS             complement(<1..286)
                     /locus_tag="VGM46_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192812.1"
                     /translation="MAFIPVPAAGFAARLFATPSWRVSGDLIEVLRVYGGKWANSGRP
                     GFARRDDTRVLLKRHSRLKRDWRRQASSDIQMARKKAGRPGLFLSDQPFFS"
     gene            495..1316
                     /locus_tag="VGM46_09230"
     CDS             495..1316
                     /locus_tag="VGM46_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019170481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY4192813.1"
                     /translation="MRVAELLRSLVFSALVAMATLGAVDRALAFDDKVFDDKTGVKPE
                     SSPWAVFQFGFSAYKNGHKDQAVEAYKYAAENGQIGATWKLARMYAQGDGVTQDDYSA
                     YKFFSEIVDQDVEPGSPEESYVSDALVALGGYAMNGIPGSPVKADPVAAQEYYMRAAA
                     NYRNPDAQYEMGKMFLKGDGGVKASVKQAGRWLQLAAEKGHAGAQATLGNLLFQSGKL
                     VRGLTMMTVALARAAPTDQPWIRGMQEEAFAVAPEADRRTAISLADGILTKGASQ"
     gene            complement(1776..2570)
                     /gene="xth"
                     /locus_tag="VGM46_09235"
     CDS             complement(1776..2570)
                     /gene="xth"
                     /locus_tag="VGM46_09235"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEY4192814.1"
                     /translation="MKIVTWNINGVRARIGNLTQWLADSAPDIVCLQEIKSVDEQFPR
                     AEIEALGYNVETHGQKGFNGVAILSKLPFDEVNRGLPGDDTDEQARFIEGVFSTDKGA
                     LRVVSLYLPNGNPISDERKFPYKLSWMARLERWAAQRLELEEALVLAGDYNVIPEAID
                     ARHPENWLGDALFQPETRQAFRRLLNLGFTEAVRAVNDAAEVYTFWDYQAGAWQKNNG
                     IRIDHLLLSPEAANRFSSADIEKHVRAWEKPSDHVPVAIQLALRAA"
     gene            2711..3835
                     /locus_tag="VGM46_09240"
     CDS             2711..3835
                     /locus_tag="VGM46_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY4192815.1"
                     /translation="MGDRPAIALSRRSFLGQAAAFGIVGVARPAFGQTQGIPPADATF
                     LFVADIHACRMAKGLSPQCQQEGKTDASLLRNIAALNGIGGKLWPSEIGGVATGLRSA
                     GQSIATPLGIVVGGDMTDDGGGQITLPSEGTQLLQFSQRYQQGVGPDRVHFPVYAGLG
                     NHDLDQNGPKNHVDWYRRELRDYVEINHRAGIFFKPPVPADNYDVDSDCYSWNWGGLH
                     LVQTHRFAGDTGHGAASGLPWLKRDLAANAADGRPVVLFQHYGWDVFSTEVWDAAQHE
                     FDDNGNGPPHWWSEADRLALLAALKDYNVVAIFHGHQHETPMIYQRDGLDLFKPKAAY
                     MGGFALARIAGGAMDVVLGEAAGDHGEVVFTNAFSKPLRA"
     gene            complement(3881..4228)
                     /gene="erpA"
                     /locus_tag="VGM46_09245"
     CDS             complement(3881..4228)
                     /gene="erpA"
                     /locus_tag="VGM46_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015317526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEY4192816.1"
                     /translation="MGADAKTAMKIDVTDAAFKRIARIMAAEPGKSALRVSVEGGGCS
                     GFSYKFDLVSERNEDDIALEKDGATVLIDELSVVYMGGSVIDFVDDLMGQSFQIKNPN
                     AVASCGCGTSFSI"
     gene            4420..5643
                     /locus_tag="VGM46_09250"
     CDS             4420..5643
                     /locus_tag="VGM46_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HEY4192817.1"
                     /translation="MTDDKAALGDIGFGYRPRAAYAADPASSRGRLFPEPESPTRTPF
                     QRDRDRIVHSTAFRRLKHKTQVFVAHEGDHYRTRLTHSIEVAQIARALSRSLRGDEDL
                     TEAVALVHDFGHTPFGHTGEDALNEKMAAWGGFDHNAQSLRIVTRLEHRYAEFDGLNL
                     AWETLEGLVKHNGPLTDAGGQGLHGPVPQTIRAYNELHDLELARHAGIEAQCAAIADD
                     IAYNTHDIDDGLRAGLLTLEMLEELALPGSILKDVREKYPTLDPVRTGHELMRRQITR
                     MVEDVIVTTHANLARIDPQSADDVRGAGQTMVAFSPAMAAGEKELKAFLYRRLYRHEQ
                     VMRVRADAETIVKQLFDVYFADPRAMPDGWREGLDRAEDRIKARHVADFLAGMTDTYA
                     IKEHQRLFDHTPDLG"
     gene            5727..7484
                     /gene="argS"
                     /locus_tag="VGM46_09255"
     CDS             5727..7484
                     /gene="argS"
                     /locus_tag="VGM46_09255"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009363403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEY4192818.1"
                     /translation="MNIFADFTARIIKTVEALDLKDKDGASPDLSRVAVEPPRDATHG
                     DLATNAAMVLAKPTGQNPRALAEKLAEMLRQDKDVAAAEVAGPGFVNLRLRDGFWQAH
                     LASLIGAGKDYGRSTVGQGHKVNVEYVSANPTGPMHVGHCRGAVVGDALANLMAFAGY
                     AVTKEYIINDAGSQIDVLARSVMLRYREALGEVVGEIPPGLYPGDYLVPVGQALAAEF
                     AGKLLQMPDDEALAIIKDRTIDAMMAMIKSDLALLNVHHDVFFSERTLHADNAKKIRS
                     AINDLTLKGHIYKGTLPPPKGEKPDDWEDREQTLFRSTAVGDDLDRALIKSDGSFTYF
                     AADVAYLKDKVDRGFVDLIYVLGADHGGYVKRLEALARAVAGDRVKLTVLLCQLVKLF
                     RNGEPVRMSKRSGDFVTLREVVEEVGRDPIRFMMLYRKNDAPLDFDFAKVTEQSKDNP
                     VFYVQYASARCYSVFRQAAEQLGEAEFDRKELAAASAGLTDEGELGLIRKLAEYPRLI
                     ESAALSLEPHRLAFYLYDLASAFHGHWNRGTENPDLRFVKVNDRQLTLARLGLVQAVS
                     DVLSSGLALIGAEAPTEMR"
     gene            7588..>8069
                     /locus_tag="VGM46_09260"
     CDS             7588..>8069
                     /locus_tag="VGM46_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015317529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192819.1"
                     /translation="MADRTPLRTADRNAISNAISDDDPFAELTRIMGFDPRQPVQARQ
                     DPVMADSDFEIDLEKELMGEFSVEADDDFSPEQNAQNAQGAYEAPAVSHGHLPARDLP
                     AHDLDQTVAASMQDFDLDMSGEAAQPAAPEIGFEDDFEDALAQSFDAEPQWPEETENP
                     A"
BASE COUNT         1534 a         2515 c         2491 g         1529 t
ORIGIN      
        1 cgctgaaaaa aggctggtcg ctgagaaaaa ggccgggtct cccggccttt tttctggcca
       61 tttgaatgtc ggaagacgct tggcgccgcc aatctcgctt taatcggctg tgtcgtttta
      121 gcaacacacg cgtgtcatcc cgccgcgcga accctggcct gcccgaattt gcccatttcc
      181 cgccataaac ccggagcacc tcaatcaggt ctccggacac gcgccatgaa ggcgtggcga
      241 agagacgggc cgcaaagccg gccgcaggga ctggaatgaa cgccatagcc accttctcca
      301 tcgataggcc atgccgtgcg cgccgcgcac cggcatgtcg tactttggac gaaaagccgg
      361 tgcaggctcc ctccgcaaac cgaccggcca tgcgggcacg cgcagttctt tccggcatga
      421 tctcgaccgg gtcggatatg acgaggcggc gccgcaagac ggcggccggc gcgcggcgca
      481 acgagcacgg gttcatgcgg gtagcggagt tgttgaggtc actggttttt tcggcgctgg
      541 tcgcgatggc gacgctgggc gccgtcgatc gggccttggc tttcgatgac aaggtttttg
      601 acgacaagac cggggtaaag ccggaatcca gcccttgggc ggtgttccag ttcggcttct
      661 ccgcctacaa gaacggccac aaggaccagg ccgtcgaagc ctataaatat gccgccgaaa
      721 acggccagat cggcgccacc tggaagcttg cccgcatgta tgcgcagggc gatggcgtga
      781 cgcaggacga ctactccgcc tataaattct tcagcgagat cgtcgatcag gacgtcgagc
      841 ccggctcgcc cgaggaaagc tatgtctccg acgcgctggt tgcgctgggc ggctatgcca
      901 tgaacggcat ccccggttcg ccggtgaaag ccgatccggt tgcagcgcag gaatattaca
      961 tgcgcgccgc cgccaactac cgcaatcccg atgcccagta cgaaatgggc aagatgttcc
     1021 tgaagggcga tggcggcgtc aaggccagcg tcaagcaggc cgggcgctgg ttgcaactgg
     1081 ccgccgaaaa gggccatgcc ggcgcgcagg cgacgcttgg caatctcttg ttccagagcg
     1141 gcaagctggt tcgcgggctg accatgatga cggtggcgct ggcgcgcgcc gcgcccaccg
     1201 atcagccatg gattcgcggc atgcaggaag aggcgttcgc cgtagcgccc gaagccgacc
     1261 gccgcaccgc catttcgctg gccgacggca tcctcaccaa aggcgccagc caataggctt
     1321 ggctgtctga aatctactag atcggttccg ttttttaccg aatcgacaaa gccgctctat
     1381 ctctttgttt ctgctgcatt tccggacgga aacaccgttt cacgtttttg ctggaaatct
     1441 actagttttc ggttggttcg cttggcatgg ccggcgaagc gccaggttcg gaggccggct
     1501 ttgtctccgg cgacgcgtcg ttttgcgaac agccgtcctg tatttttgtc cacgacgtat
     1561 tgttgaggac catatcgcat cgggcgagat ggctcttgcc ggaaagtgtg atgcattcct
     1621 tctggccgat ttcgaaggac gtgctgttcg ccaggcattt gggcgcggcc atcgccggcg
     1681 cggttgccat aaccgtcaaa accaacgccg ccgatgcaag tactgttctc ataagccggc
     1741 caatccaaag gtcgtttcga ctgccaaccg agccttcagg ccgcgcgcag cgccagttgt
     1801 atggctacgg gtacatggtc ggacggcttt tcccacgcgc gaacatgctt ttcgatatcg
     1861 gccgacgaaa accggttggc ggcttccggc gacagaagca gatggtcgat gcggatgccg
     1921 ttgttcttct gccatgcgcc ggcctgataa tcccagaacg tatagacttc ggcggcgtcg
     1981 ttcaccgcac gcaccgcttc ggtgaagcca aggttcagca gccgccggaa agcctgccgg
     2041 gtttcgggct ggaacagcgc atcgcccagc cagttttccg gatgccttgc atcgatggct
     2101 tcgggtatga cgttgtagtc tccggccaac accagcgcct cttccaactc cagccgctgt
     2161 gccgcccagc gctccagcct tgccatccac gacagcttgt aggggaattt gcgctcgtcg
     2221 ctgatcgggt tgccgttcgg caggtacagc gagacaacgc gcaaagcgcc tttgtcggtg
     2281 gagaaaaccc cttcgatgaa gcgcgcctgc tcgtccgtgt cgtcccccgg caggccgcga
     2341 ttgacttcgt cgaatggcag tttcgacagg atggcgacgc cgttgaagcc cttctggcca
     2401 tgcgtctcga cattgtagcc cagcgcctcg atctcggcgc gggggaactg ctcgtccacc
     2461 gacttgattt cctgaaggca cacgatgtcg ggcgcgctgt cggcgagcca ttgcgtcagg
     2521 ttgccgatgc gcgctcgcac gccgttaatg ttccaggtaa cgatcttcat gaaaatcacg
     2581 cgataatttc aaagacttct tgccggcgcg gacgccttgc agacgatcct gcaccattgc
     2641 tccggacgag gaaacccgat tttatcctga ggacccgatt ttatcttgag gaacagaaaa
     2701 gcgaggcaat ttgggcgatc gaccggcgat agcgctttcg cgccgcagtt tcctggggca
     2761 ggcggctgcc ttcgggatcg tcggcgtcgc gcggccggca ttcggccaga cgcagggcat
     2821 cccgccggca gacgccacct tcctgttcgt tgccgatatc cacgcctgcc gcatggccaa
     2881 gggcctcagc ccgcaatgcc agcaggaggg caagaccgac gccagtctat tgcgcaacat
     2941 cgcagcgctg aacggtatcg gcggcaagct ttggccgtcc gaaatcggcg gtgtcgcgac
     3001 gggcctgcgt tcagccgggc aatcgatcgc tacgccgctt ggaatcgtcg tcggcggcga
     3061 catgaccgac gatggcggcg ggcaaatcac tctgcccagc gaaggcacgc aattgctgca
     3121 attcagccaa cgctaccagc aaggcgtcgg accggatcgc gttcattttc ccgtttatgc
     3181 agggcttggc aatcacgatc tcgaccagaa cggccccaaa aaccatgtcg actggtatcg
     3241 gcgggaactg cgcgactatg tcgaaataaa ccaccgcgcc ggcatattct tcaagccgcc
     3301 ggtgccggcc gacaactacg atgtcgattc tgactgctat tcctggaatt ggggtggcct
     3361 gcaccttgtg cagacgcacc gttttgccgg cgataccggc catggcgccg ccagcggctt
     3421 gccgtggctg aagcgggatc tggcggcaaa cgccgcggat ggacggcctg tcgtgctctt
     3481 ccagcactat ggctgggatg ttttttcgac cgaggtttgg gacgcggccc agcacgaatt
     3541 cgacgataac ggcaacggcc cgccgcattg gtggagcgag gcggacaggc tggcgctgct
     3601 ggccgcactg aaagactaca atgtcgtcgc catctttcac ggccatcagc atgagacgcc
     3661 gatgatctac cagcgcgacg ggcttgacct gttcaagccc aaggccgcct atatgggcgg
     3721 cttcgcattg gcgcgaatcg cgggcggggc gatggatgtc gtgcttggcg aggcggcggg
     3781 cgaccacggc gaagtggtct tcaccaacgc cttcagcaag ccgttgcgcg catgaaaaag
     3841 gccgcatgcg cggccctttt ttgatttcgg tcggtaaatg tcagatcgag aaactggtgc
     3901 cgcagccgca ggaggcgacc gcgttcgggt tcttgatctg gaacgactgg cccatcaggt
     3961 cgtcgacgaa atcgattacc gatccgccca tatagaccac cgaaagctcg tcgatcagca
     4021 cggtggcgcc gtctttttcg agcgcgatat cgtcttcatt gcgctcgctg acgaggtcga
     4081 acttgtagga aaagccggaa cagccgccac cttcgacgga cacgcgcaac gcgctcttgc
     4141 ccggttcagc cgccatgatc ctggcgatgc gcttgaaggc ggcgtcggta acgtcgatct
     4201 tcatggcagt ctttgcatca gcgcccatcg gcgtcacctt gctttcggtt gttcatgata
     4261 ggtatgaagc gcgaagggcc aagtcaacgg gtggcgtgac gagatattgc caacgccggc
     4321 catcggctgg tccgcgggaa cgatgctggc tatctgacgg gtgctgcctg acgggtgctg
     4381 cctgacgggc gctgaatgac gggtgttgaa tgatgggcca tgactgacga caaagccgct
     4441 cttggcgata tcggtttcgg ctatcgcccc cgcgcggcct atgccgccga tccggcaagc
     4501 tcgcgcggcc gcctgtttcc cgagccggaa agcccgacgc gcacgccttt ccagcgcgac
     4561 cgcgaccgca tcgtccattc caccgccttt cgccgcctga aacacaagac gcaggtgttc
     4621 gttgcccacg agggcgatca ttaccgcacg cggctgaccc attcgatcga ggtggcgcag
     4681 atcgcgcgcg ccctgtcgcg ctcgcttcgc ggcgacgagg atttgaccga ggcggtggcg
     4741 ctggtgcacg atttcggcca cacgccgttc ggccatacag gcgaagacgc gctgaacgag
     4801 aaaatggccg cctggggcgg tttcgaccac aatgcgcagt cgctgcgcat cgtgactagg
     4861 ctcgaacacc gctacgccga attcgacggg ctgaaccttg cctgggaaac gctcgaaggc
     4921 ctggtcaagc acaacggccc gctgaccgat gctggcgggc aagggctgca tggcccggtg
     4981 ccgcagacca tccgcgccta caatgagctg cacgatctcg aactcgcccg ccacgccggc
     5041 atcgaggcgc aatgcgccgc gattgccgac gatatcgcct acaacaccca tgatatcgac
     5101 gatggcctgc gcgccggcct gctcactttg gagatgcttg aggaactggc gctgcccggt
     5161 tcgatcctga aggacgtgcg cgaaaaatac ccgacgctcg atccggtgcg caccggccat
     5221 gagttgatgc gccgccagat cacccgcatg gtcgaggatg tgatcgtcac cacccatgcc
     5281 aacctggcgc gcatcgatcc gcaaagcgcc gacgacgtgc gcggggccgg gcagacgatg
     5341 gtggcatttt cacccgcaat ggctgccggg gaaaaggaac tgaaggcgtt tctctaccgt
     5401 cgcctttatc gccacgaaca ggtgatgcgg gtgcgcgccg acgccgaaac gatcgtcaag
     5461 caattgttcg atgtctattt cgccgatccg cgcgccatgc ccgatggctg gcgcgagggg
     5521 ctggaccgcg ccgaggaccg catcaaggcg cgccacgtcg ccgatttcct ggccggaatg
     5581 accgacacct acgccatcaa ggaacaccag cgcttgtttg accatacgcc ggatttgggc
     5641 tagcgctgcg caaaatcact tctgacgtga attcacgttt ctccgagagt tcgctgaatt
     5701 ctcccgcatg tctatgaaag cctgccatga acatcttcgc cgacttcacc gcgcggatta
     5761 tcaagaccgt cgaagcgctt gacctgaagg acaaggacgg cgcgtcgccc gatttgtcgc
     5821 gcgttgccgt cgagccgccg cgcgacgcca cccatggcga tctggcgaca aatgcggcca
     5881 tggttttggc caagccgacc ggccaaaacc cgcgcgcttt ggctgaaaaa ctggctgaaa
     5941 tgctgcgcca ggacaaggat gtcgccgccg ccgaggtggc cgggccgggc ttcgtcaacc
     6001 tgcggctgcg cgatggcttc tggcaggcgc atctggccag cctgatcggc gccggaaaag
     6061 attatggccg ctcgaccgtg gggcaggggc acaaggtcaa tgtcgaatat gtctccgcca
     6121 atccgaccgg cccgatgcat gtcggccatt gccgtggcgc cgtggtgggc gatgcgctgg
     6181 ccaatctgat ggccttcgcc ggctatgccg tcaccaagga atacatcatc aacgacgccg
     6241 gctcgcagat cgatgtgctt gcccgctcgg tcatgctgcg ctaccgcgag gcgctgggcg
     6301 aagttgtcgg cgaaattccg ccggggcttt atccgggcga ctatctcgtc ccggtcggcc
     6361 aggcgctcgc cgccgagttt gccggcaagc tgcttcagat gccggatgac gaggcgctgg
     6421 ccatcatcaa ggaccgcacc atcgacgcca tgatggcgat gatcaagagc gatctggcgc
     6481 tgctcaacgt ccatcatgac gtgttctttt cagagcgcac gctgcatgcc gacaatgcca
     6541 agaagatccg ctcggccatc aacgacctga cgctcaaggg ccacatctac aagggcacgc
     6601 tgccgccacc caagggcgag aagcccgacg actgggaaga ccgcgaacag accctgtttc
     6661 gctcgacggc ggttggcgac gacctcgacc gcgcgctcat caagtccgat ggctccttca
     6721 cctatttcgc cgccgatgtg gcctatctga aggacaaggt cgatcgcggt ttcgtcgacc
     6781 tgatctacgt gctgggcgcc gaccatggcg gctacgtcaa gcggctggag gcgctggcgc
     6841 gcgcggttgc cggcgacagg gtcaagctca ccgtcctgct ttgccagctg gtcaagctgt
     6901 tccgcaatgg cgagccggtg cgcatgtcca agcgttcggg cgatttcgtc acgctgcgcg
     6961 aagtggtgga ggaggtcggc cgcgacccca tccgcttcat gatgctctac cgcaagaacg
     7021 acgcgccgct cgatttcgac ttcgccaagg tcaccgagca gtcgaaggac aatccggtat
     7081 tctacgtgca atacgcctcg gcgcgctgtt actcggtttt ccggcaggcg gcggagcaat
     7141 tgggcgaggc ggaattcgac cgcaaggaac ttgccgccgc aagtgccggc ctcaccgacg
     7201 agggcgagct tggcctcatc cgcaagcttg ccgaatatcc gcgcctgatc gaatccgcgg
     7261 ccctttcgct ggagccgcac cggctggcat tctacctcta cgatctcgcc agcgccttcc
     7321 acggacactg gaaccgcggc accgaaaatc cggacttacg gtttgttaag gttaacgacc
     7381 gacaattgac gcttgccagg ctgggtctgg tgcaggctgt ttcggatgtt ttgtcgtccg
     7441 gcctggcgtt gatcggagcg gaagcgccca ccgaaatgcg ttaggagttg ctaaaacacc
     7501 tgtcaccttt tgcccacatt gcgctggtaa gggccaacct tgcgcgacgt cgatggcgtc
     7561 cgactgtgta cgagttcggg aacaaagatg gcagacagaa ccccgctgag aaccgccgac
     7621 cgtaacgcga tctctaacgc gatctccgac gacgatccgt tcgcggaact gacccgcatc
     7681 atgggtttcg accctcgcca gccggtgcag gcgaggcagg acccggtgat ggccgacagc
     7741 gatttcgaaa tcgacctcga aaaggagctg atgggcgaat tcagcgtcga ggccgatgat
     7801 gatttttcgc cggaacagaa tgcgcagaac gcgcagggcg catacgaggc gccggccgtg
     7861 tcacatggcc atctgcccgc gcgcgatctg cccgcacatg atctggacca gacggttgcc
     7921 gcgtcgatgc aggatttcga cctcgacatg tcgggcgaag ccgcgcagcc ggccgcgcct
     7981 gaaatcgggt tcgaggatga tttcgaagat gcgctggcgc agtccttcga tgccgagccg
     8041 cagtggccgg aggaaacgga aaatcccgc
//
LOCUS       DASYCS010000293         2082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4856371,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000293 DASYCS010000000
VERSION     DASYCS010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4856371"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..1393)
                     /locus_tag="VGM46_09265"
     CDS             complement(101..1393)
                     /locus_tag="VGM46_09265"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEY4192820.1"
                     /translation="MGQTVFIPLEADAHETRVAASPDTVKRLGALGFEIIVEKGAGAL
                     SRITDDEFAKAGAVIGKATDAAKADVVLKVRRPADGELKGYKKGAAVFAIMDPYGNDA
                     AVAALAKAGVTAFSMEFMPRITRAQSMDVLSSQANLAGYQAVIDGAAEYDRALPMMMT
                     AAGTVPAAKIFIMGVGVAGLQAIATARRMGAVVTATDVRPAAKEQVASLGAKFLAVED
                     EEFKAAETAGGYAKEMSKEYQAKQAALTAEHIAKQDIVITTALIPGRPAPKLVSAAMV
                     ASMKPGSVIVDLAVERGGNVEGAVAGKVVTTANGVKIVGHLNVPGRIAASASLLYAKN
                     LYTFLETMVDKATKQFAINRDDELVKATMLTDGGKVVHPSFAKAVEPHVEPAAIPATV
                     MVADASAPLAKAAAKKPASAKKPAATKSPPVSKPKGIA"
     gene            complement(1477..1704)
                     /locus_tag="VGM46_09270"
     CDS             complement(1477..1704)
                     /locus_tag="VGM46_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006325340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aa3-type cytochrome c oxidase subunit IV"
                     /protein_id="HEY4192821.1"
                     /translation="MADHTPTGPVELGANMDYAEHERTYHGFTLLAKYGSLFCAAVLA
                     AMAFSFFTTAGFFSGTILFVLILVVGGYILR"
     gene            complement(1869..2018)
                     /locus_tag="VGM46_09275"
     CDS             complement(1869..2018)
                     /locus_tag="VGM46_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3096 domain-containing protein"
                     /protein_id="HEY4192822.1"
                     /translation="MTFSTLTLTPLISLIAGVLILVMPRLLNYIVAIYLIVVGLLGLF
                     PNLAH"
BASE COUNT          400 a          640 c          673 g          369 t
ORIGIN      
        1 gccgcatcgc ccgcgccatt gacggacaaa tcctggatgg ccatgcgcac ggcggcggag
       61 gcctgatcga gctggtcgag cgctttttgc aaggcttcca tcacgcaatc cccttcggct
      121 tcgaaacagg cggcgattta gtggccgccg gctttttcgc ggaggcaggc ttcttggccg
      181 cagcctttgc gagcggcgcg gaggcgtcgg ccaccatgac ggtcgccgga atggccgcag
      241 gctcgacatg cggctcgaca gccttggcga aggaaggatg caccaccttg ccgccatcgg
      301 tcagcatcgt cgccttgacc agttcgtcgt cgcggttgat ggcaaactgc ttcgttgcct
      361 tgtcgaccat cgtttcaagg aaggtgtaca ggttcttggc gtagagcagc gaggcagagg
      421 cggcgatgcg gcccggcacg ttgagatggc cgacgatctt gacgccattg gcggtggtga
      481 ccaccttgcc ggccaccgcg ccctcgacat tgccgccacg ctcgaccgcc agatcgacga
      541 tgaccgagcc gggcttcatc gaggcgacca tcgcggcgga aacgagtttc ggcgcgggac
      601 ggccggggat cagcgcggtg gtgatgacga tatcctgctt ggcgatatgc tcggccgtca
      661 gcgcggcctg cttggcctgg tattccttcg acatttcctt ggcatagccg ccggcggttt
      721 cggcggcctt gaattcctcg tcctcgacgg cgaggaattt cgcgccaagc gaagcgacct
      781 gctccttggc ggcggggcga acgtcggtgg cggtaacgac cgcgcccatg cggcgggccg
      841 tggcgatggc ctgaaggccg gcaacgccga cacccatgat gaagatcttc gccgccggca
      901 cggtgccggc cgccgtcatc atcatcggca atgcgcggtc atattcggca gcgccgtcga
      961 tcaccgcctg atagccggcg agattggcct ggctggacag cacgtccatc gactgcgcgc
     1021 gggtgatgcg cggcatgaat tccatggaaa acgcggtgac gccggccttg gcaagcgccg
     1081 cgacggccgc gtcgttgccg taagggtcca tgatggcaaa aaccgccgcg cccttcttgt
     1141 agcccttcag ttcgccatcg gcgggacggc gcactttcag caccacatcg gctttcgccg
     1201 catcggttgc cttgccgatg accgcgccgg ccttggcgaa ttcgtcatcg gtgatgcgcg
     1261 acaacgcgcc tgcgcctttt tcgacaatga tttcaaagcc gagagcgcca aggcgcttca
     1321 ccgtatccgg cgaggccgca acgcgcgttt catgcgcatc cgcttcgaga gggatgaata
     1381 cagtctgtcc cacgcgacat cctttcggct tgtgcttcat gtccggccag gcagccggcc
     1441 gcgccggtta aggcgcgctg gaaaagagcc gtgggcctag cggaggatat aaccgccgac
     1501 gaccaggatc agaacgaaga ggatcgtccc ggaaaagaaa ccagccgtgg tgaaaaagct
     1561 gaaggccatg gcggcgagga cggccgcgca gaagagcgag ccatatttgg ccagcaaggt
     1621 gaagccgtga taggtgcgct catgctccgc gtagtccata ttcgcgccca attcgaccgg
     1681 gccggtcggg gtgtggtcag ccatgagaat accctccgag aaagtctctt gcgcacatag
     1741 cgaaaagaca cgccgagggc aatgggtgtg atgccgcacc gcggcagatc acgtcatcaa
     1801 atgaatatgg ggcggcggca tcgaagccgg gccgatgcgc cgccctatca ggcaggcatg
     1861 cgaaccgctc aatgcgctag atttggaaac aggccaagca gtccgaccac gatcaggtag
     1921 atcgccacga tgtaattcaa cagccgtggc atcaccaaaa tgagcacgcc tgcgatcagc
     1981 gagatcagcg gcgtcaaggt cagggtagaa aaggtcattt tcagctccgc gtttcagttt
     2041 ccgccacccg gtaacggcga caggggccga aagctccatg gc
//
LOCUS       DASYCS010000294         4074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3412388,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000294 DASYCS010000000
VERSION     DASYCS010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3412388"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..173)
                     /locus_tag="VGM46_09280"
     CDS             complement(<1..173)
                     /locus_tag="VGM46_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006329336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY4192823.1"
                     /translation="MAYQSPISPGRSWYEDSVGPRLRYPALDGDRRADVVIVGGGFTG
                     LSAAVHLAKAGIDV"
     gene            complement(235..599)
                     /locus_tag="VGM46_09285"
                     /pseudo
     CDS             complement(235..599)
                     /locus_tag="VGM46_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006469281.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
     gene            complement(620..2056)
                     /locus_tag="VGM46_09290"
     CDS             complement(620..2056)
                     /locus_tag="VGM46_09290"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018426576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEY4192824.1"
                     /translation="MMPPAKKETRPSNRAGSVRTPAFVKNHRGVKNWKEASAWLEYRG
                     IEDIECITPDQAGIARGKMMPSKKFTSNTSLALPSAVFMATISGGYPENGNGFHYPED
                     DGDLKLMPDLSTLTVVPWEEDPTAAAICDLVHQDGRSVEFAPRNVLKRVLDAYSKLGL
                     KPVVAPEIEFYLVRKNPDPDYPLVPPVGRSGRAIGGGAGYSIAGVNEFDELIDDIYHF
                     SESQGLEIDTLIHEEGAGQLEINLRHGDPIELADQVFMFKRTIREAALKHEIYATFMA
                     KPIQGQPGSAMHIHQSILDKKTGKNIFTAEDGSETDAFFHFIGGMQKHVPNALAMFAP
                     YVNSYRRLTQAASAPVNNKWGYDNRTTAFRVPRSDPAARRVENRIPSSDANPYLALAA
                     SLACGLIGMTNKIKAEPPVLTTANEDQIDLPRGLLEAVDLLEADEVLCEMLGKSFTST
                     YAAIKRAEFETFMEVISPWEREYLLLNV"
     gene            2247..3494
                     /locus_tag="VGM46_09295"
     CDS             2247..3494
                     /locus_tag="VGM46_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009210405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X"
                     /protein_id="HEY4192825.1"
                     /translation="MALELDARDQSMLDGEHGAAVAAAMKILVAFADAVGAQRLLDIS
                     SAHIDGCLYHGKAGLDFVERLVEGGGRVAVPTTLNVGSFDLIHPGMVKMPAGDEAPAR
                     RLMKAHIELGCQATFTCAPYQTRFRPRFGEQIAWGESNAIVFANSVIGARTNRYGDFI
                     DLCCAMTGRAPAWGLHLSENRRGRILFELADALEPSDSVFVGVGLVVGHKSGDRVPVI
                     AGLPQPRDEDQLKAFGAAAATTGAVALFHAVGITPEAATLDEAFHGLPPEQTIHISRA
                     DIAGALASLSSVPDGAPLSAVSLGTPHFSHEEWLRLLPMLREIAPAGNIPIYVNTGRA
                     TLQRLQDEGALAGMEAFGLTPVADTCTYVTAILGRLEGVVMTNSGKWAHYAPGNIGVS
                     VAFGDMEDCIRSAAAGHVVRGRL"
     gene            3491..3922
                     /locus_tag="VGM46_09300"
     CDS             3491..3922
                     /locus_tag="VGM46_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HEY4192826.1"
                     /translation="MKRFATRTGVFQLAGEAQGQALVFSQALSFWGGIDAGTGDIIDH
                     SHPGLGLNVAGKVLVMPSGRGSSSSSSVLAEAIRCGTAPAGIIIERLDPILAVGAIVA
                     EFLYAIRIPLVVCDIAGIVSGDPISIRSNGAGQAVVTLPAA"
BASE COUNT          703 a         1257 c         1316 g          798 t
ORIGIN      
        1 acatcgatgc cggccttggc cagatgcacc gccgccgaca ggccggtaaa accgccgcca
       61 acgatgacca catcagcccg ccggtcacca tccagcgccg gatagcgcaa gcgaggcccg
      121 acgctgtcct cgtaccagga acggccgggc gagatggggg attggtaggc catgagcggg
      181 gggtccgagt agggcagtag ggcagtaggg cagtagggca gtagggcagt aggatcaggt
      241 ttccgacagc ttgcggatga gaagacgcag caacttgctg acgctttcac ttgcgcccat
      301 cggcggtgcg attgtcgcgg ctgaagcgcc gacgcgttct gcgatttgta ggtgcgtctc
      361 cagttccttg agcgatcctt gggcaattct caggaaattc tgataggagg ccctgctttc
      421 ctgccatagc cttcggcgat attggcggga acggatacag ctgccctacg cgcttgggcg
      481 gtcagtccac agagttcgtc cttcggccaa cctcgcgtaa tttcataagt cgaaaccgcc
      541 aggtccatgg cctgctgcca aacaagaaga tccctatacg aatcgatctt tgaccgcatc
      601 tctcccctac tcccctgcct cacacattca gcagcagata ttcccgctcc cacgggctga
      661 tgacttccat gaaggtctcg aattccgccc gtttgatcgc cgcataggtg ctggtgaagg
      721 attttcccag catctcacac aacacttcgt ccgcctccag caggtcgacc gcttcgagca
      781 atccgcgcgg caggtcgatc tggtcttcat tggccgtggt cagcacgggc ggttcggcct
      841 tgatcttgtt ggtcatgccg atcaacccgc aggccagcga tgcggccagc gccagatagg
      901 ggttggcgtc ggaggaaggg atgcggtttt cgacgcgccg cgccgccggg tcggagcgcg
      961 gcacgcgaaa cgccgtggtc cggttgtcgt agccccattt attgttgacc ggcgcggaag
     1021 ccgcctgcgt cagccggcgg taggaattca catagggcgc aaacatcgcc agcgcgttcg
     1081 gcacatgctt ctgcatgccg ccgatgaaat ggaagaaggc gtccgtctcc gagccgtctt
     1141 cggcggtgaa gatgttcttg ccggtcttct tgtcgaggat agactggtgg atgtgcatgg
     1201 ccgaacccgg ctggccctgg atcggcttgg ccatgaaggt ggcatagatt tcgtgcttca
     1261 gcgccgcttc gcggatggtg cgcttgaaca tgaaaacctg gtcggcaagc tcgatcggat
     1321 cgccatggcg caggtttatc tccagctggc cggcgccttc ctcgtggatc agcgtgtcga
     1381 tctccagccc ctggctttcg gagaagtggt agatgtcgtc aatcagttcg tcgaactcgt
     1441 tgacgccggc gatcgaatag ccggcgccgc caccgatggc ccggccggaa cgcccgaccg
     1501 gcggcaccag cggatagtcg gggtcggggt tcttgcgcac cagataaaat tcgatttccg
     1561 gcgctaccac cggcttcagg ccaagcttgc tataggcgtc gagcacgcgc ttgagcacgt
     1621 ttcgcggcgc gaattcgacc gaacgtccgt cctggtgaac gagatcgcag attgcggcgg
     1681 cggtcgggtc ttcttcccac ggcaccacgg tcagcgtcga aaggtccggc atcagcttca
     1741 gatcgccatc gtcttccgga tagtggaaac cgttgccgtt ttccggatag ccgcccgaaa
     1801 tggtcgccat gaacacggcc gatggcaggg ccagcgaggt gttggaggtg aacttcttcg
     1861 acggcatcat cttgccgcgc gcaatgccgg cctgatcggg cgtgatgcat tcgatgtctt
     1921 cgatgccgcg atattccaac caggcgctgg cctctttcca gttcttgacg ccgcgatggt
     1981 tcttgacgaa agcaggcgtg cgaacgctcc ccgcacggtt ggacggtcgg gtttcctttt
     2041 tagcaggcgg catcattcac cagatcgctt gagtgtgccg gagtataggc gctccgcgtg
     2101 gcgaaaggaa aggggccggc cgattagcat cgccgcaatg cgcctgacgg tgcaaacggc
     2161 cgagcctggt tctgccgatt ttcaaggttg cctcggtcgc caccgcttcg ggcactgtgt
     2221 aaggatgtag ccgatggagg atgccgatgg cgcttgaact tgatgcgcgc gaccagtcga
     2281 tgctcgacgg cgaacacggc gccgccgtag ccgccgcgat gaaaatcctc gtcgccttcg
     2341 ccgatgccgt cggcgcgcaa aggctgttgg acatttccag cgcccatatc gatggctgcc
     2401 tctatcacgg caaggccggc ctcgatttcg tggagcggct ggtcgagggc ggtggccgcg
     2461 tggcggtgcc gacgacgctg aatgtcggct cgttcgacct catccatccg ggcatggtca
     2521 agatgcccgc cggcgatgaa gcgccggcgc gccggttgat gaaggcccat atcgagcttg
     2581 gctgccaggc gaccttcacc tgcgcgccct accagacgcg gtttcgcccg cgcttcggcg
     2641 agcagatcgc ctggggcgaa tccaacgcca tcgttttcgc caattcggtg atcggcgcgc
     2701 gcaccaaccg ctatggcgat tttatcgacc tgtgctgtgc catgaccggg cgcgcgccgg
     2761 cctggggcct gcacctgagc gagaaccggc gaggccgcat cctgttcgaa ctggccgatg
     2821 cgcttgaacc gagcgacagc gtgttcgtcg gcgtcgggct ggtcgtcgga cacaaaagcg
     2881 gcgaccgcgt tccggtaatt gccgggctgc cgcagccgcg cgacgaggac cagttgaagg
     2941 catttggtgc cgccgcggcg accaccgggg cggtggcgct gtttcatgcg gttggcataa
     3001 cgccggaagc ggcaacgctg gacgaggcat tccacggctt gccgccggaa cagacgatcc
     3061 acatttcgcg cgccgacatt gccggtgcgc tggccagcct gtcgagcgtg ccggacggcg
     3121 cacctctgtc ggccgtatcg ctaggcacgc cgcatttctc acatgaggaa tggctgcgcc
     3181 tgttgccgat gctgcgcgaa attgcgcccg ccggaaatat cccgatctac gtcaacaccg
     3241 gacgcgcgac cttgcagcgc ctgcaagacg agggcgcctt ggccggcatg gaggcattcg
     3301 gcctgacgcc ggtggccgac acatgcacct atgtcacggc gatactggga cggcttgagg
     3361 gcgtcgtgat gaccaattcc ggcaaatggg cgcactacgc gccgggcaat atcggcgtat
     3421 ccgtcgcctt cggcgacatg gaggattgca tccgttccgc cgccgccggg catgtagttc
     3481 ggggccggct atgaagcgct tcgccacgcg caccggagtt tttcagctgg ccggcgaggc
     3541 gcaagggcag gcgctggtgt tttcgcaagc gctcagcttc tggggcggca tcgacgccgg
     3601 aaccggcgac atcatcgacc attcgcatcc gggccttggg ctaaacgtcg ccggcaaggt
     3661 gctggtcatg ccgagcgggc gaggttcctc gtcctcttcc tcggtactgg ccgaggcgat
     3721 ccgttgcggc acggcgccgg ctggaatcat catcgagcga ctggatccga tcctggcggt
     3781 cggcgccatc gtcgccgaat ttctttatgc catccgcata ccgctggtgg tctgcgacat
     3841 tgcgggcatt gtctccggcg atcccatatc gatccgctca aacggcgctg gccaggccgt
     3901 tgtaacgctg ccggcggcct agcttctagg ccgcgttccg catcagatgg gtgcgcagag
     3961 cggccaggcc ttcttcgatg ttggcgcgtc cgaaaccgat gcggaaacgc ccgtccggcg
     4021 tcgggttgag ttccgattgg tagatgcttg ccggcaacag caaaacgccg gagc
//
LOCUS       DASYCS010000295        10612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1385398,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000295 DASYCS010000000
VERSION     DASYCS010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 10612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10612
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1385398"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="VGM46_09305"
     CDS             complement(<1..563)
                     /locus_tag="VGM46_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002965910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEY4192827.1"
                     /translation="MAGNFSFDQLKKAVSNGEIDTVLACIVDMQGRLAGKRFLAHYFV
                     DSAHDETHGCNYLLAADIDMEPVPGYKAASWSKGYGDFVMKPDLATIRRIPWLEKTAL
                     VICDVLDHHTHDDLPHSPRAILKKQVKRLQDRGYIGYFASELEFYLFNETYDSARGKH
                     WQGLATASPYIGDYQIGITTKEEGVMRR"
     gene            complement(651..2204)
                     /locus_tag="VGM46_09310"
     CDS             complement(651..2204)
                     /locus_tag="VGM46_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008124578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY4192828.1"
                     /translation="MAARDYTDVDKSEDVKVLHSMGYAQELERRLSRFSNFAVSFSII
                     CILSGGINSLAQGTSGAGGIGIGIGWLVGCFVSLTFAVAMSQISSAYPTAGGLYHWGS
                     ILGNRGTGWVTAWLNLLGLVTVLGAINVGTWTFFVGAFGPALGIEGTLTNQMIFLVII
                     TGAQALINHLGIKLTAKLTDFSGYLIFFGAILIAVVCLISAQTWDFARLFTFHNYSGE
                     AGGNVWPAVSNAWVFALGLLLPIYTITGYDASAHTSEETIKAASSVPRAMVMSVIWSA
                     LFGYLFLASFVLMIPNMDEAAKQGWNVFFWAFDQQVNPVVKEIVYIVVFLAQLLCGLA
                     TVTSASRMIFAFSRDGGLPASGALSKVSPTYRTPVAAIWTAAVLAVLFVWGSTLVSVA
                     GTSAYTIVVSCTVIFLFLSFTVPIVLGMMAWGTPKWDKMGPWNMGKGVFMLFGILSLI
                     SMVLIFVIGIQPPNDWALYITVGFFILTAIIWFAFEKRRFQGPPIGEIVAARQAAIAA
                     AEKAVGERS"
     gene            complement(2226..3041)
                     /locus_tag="VGM46_09315"
     CDS             complement(2226..3041)
                     /locus_tag="VGM46_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018429393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HEY4192829.1"
                     /translation="MGREPDSLAISDTVRVTNPGGSSPYLFTCDHASNFVPEEFGTLG
                     LAAAELERHIAWDPGALPVARTMAQMLDATLVESCVSRLVIDCNRPLDAPNLIAETSE
                     GTVIPGNERLTLALRQARIDLAWQPFHETIAATVEARLAAGRETRIVSMHSFTPVYLG
                     KARPWHIGIIHDEDERIAAPLLRALGGVGGIVVGANEPYSPADLVYFTLERHARPRGL
                     ACAMIEIRNDEISDAAGQQKWAKRLAGIFSRMEEDAMERNWALPAGLSARSVN"
     gene            3184..4011
                     /locus_tag="VGM46_09320"
     CDS             3184..4011
                     /locus_tag="VGM46_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006339009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEY4192830.1"
                     /translation="MASNIAELISERMAAMPAGERRAAQTLVANYPLIGLKTVADFSQ
                     QAGVSSPTILRFVARLGFHNYSEFQSALQDELAAQLQSPASRTLAPATAGGGEVSPLL
                     EATLDNMRETFRHLSDAQLGDIAARLCDLRGHVYLVGGRFTDPLARYMAAHLAIIRPN
                     VFHLTGQEIAWRDRLIDMGKRDVLMIFDIRRYQESLIRFSEKAHERGVQIVLFTDQWL
                     SPIARFARHVIAGRTAVPSAWDSSASLFVVTEALVGAVTRRLETDTARRLREMEELR"
     gene            4208..5353
                     /locus_tag="VGM46_09325"
     CDS             4208..5353
                     /locus_tag="VGM46_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006206242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY4192831.1"
                     /translation="MAAWKQIFFALVVVVAAAAAWVRFFPGAPAVLDRWGIEWAHASI
                     ATPGEATAAKAGGAKSSIPVVTALVSTATINGRLQAIGSGRAKATVTVNPYAAGRLTE
                     FLVQSGSHVEKGQTVARLDSDTETIAVDRAKVGVQDAQSKLDRVKRLQATRTASLVAV
                     SDAEVTLDNARLQLREAELALERRSIVAPIGGTVGILPMEAGNYVTSQSAVATIDDRT
                     AILVDFWVPERFAASIKVGAPITATPIASPKDVFQGSVAAVDNRVDEKSRTLWVQALI
                     ENPADSLRAGMAFDVTMKFPGDSYPAVSPLAIQWGTDGAYVWAVEDGKTKRVAVRIIQ
                     RNTETVLIDAPVVSGDIVVTQGVQSVTEGGEVRIAGEDQRAADASGS"
     gene            5350..8481
                     /locus_tag="VGM46_09330"
     CDS             5350..8481
                     /locus_tag="VGM46_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY4192832.1"
                     /translation="MTEVHNNANEAGFTALFVRRPVMALVLNTLIAVAGLAAFYGVEI
                     RELPDVDRAVISVTTDFEGAAAETVDRELTDTIEGTVARVSGVKSISSTSSFGRSRVT
                     IEFNDGVDLNVAASDVRDAVGRVANQMPDEADPPRIVKADANSDAVMRLAVTADNLSV
                     QDMTVIVQDQIEDTLAAVPGVADVQVYGDRDKIFRIDVDQNKLASQGFTVADLRAALA
                     SVAFDSPAGSINTTNQNLIVRTTADVTTPEEFENIVIGGNTHIRDVATVTLGPDIGQT
                     TLRSDGKTGIGIGIVRQAESNTLDISNGVKATVANLQKTLPKGMSIKITSDDAVFVKG
                     AVHEVEVALGLSVTFVLIVIYLFLLDWRATLIPALSMPVAMIGTIAAIYLAGFSVNIL
                     TLLALVLATGLVVDDAIVVLENIVRRRNQGMGPRAAAVLGAKEVFFAVIATTLTLVAV
                     FVPISFLPGQTGGLFREFGFVLAMSVLLSCVVALTLCPMLASRLLTNASAPQEVQKGF
                     GARVGGLLNGLYRRALHICLDAPWIVVLVAILFAGTAFALFGSIRQELTPTEDRSAVL
                     LRINAPQGVSLEYTTAQMEKIERLIQPIRDSGEIRSTFEFAGNNGAYNTGFMVMTLAP
                     WEERTRSQQQIMAQISDLARQVPSIRIFPVQPNSLGIRGAGNGLQFALVGNDRKALGD
                     AANNIIAAMQQDPRFQSPRLTVDPTQPQLAVAIDRQRASDLGIDITGLADTFQAMLDG
                     NDVVDVYIADRSYGVKLVSTTNPINDPTDLENIFLKTKDGRFVPMSTIATLTEKAVPP
                     SLTREQQQPSVAITSNLRSDFALGDAFGVAQVIAAPLMPQGARILPLAEAATLGESNS
                     AMVTIFGFALVIILLVLAAQFESFVSAVIIMATVPLGLACAIVALLLSGTSLNAYSQI
                     GLVLLVGVMAKNGILIVEFANQMRDRGLSVREAIEQAATIRLRPVMMTMICTVLGGVP
                     LVLASGAGAEARVALGWVIVGGLGLATISTLFLTPVAYLLLGRFVTPNIEEEARLKRE
                     LEEAAYIDMEPAE"
     gene            8590..9063
                     /locus_tag="VGM46_09335"
     CDS             8590..9063
                     /locus_tag="VGM46_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEY4192833.1"
                     /translation="MQPISLDDLVASAGKEVGVSPWRMVTQTMIDQFADATDDHQFIH
                     CDPERAARETPYGGTIAHGFLLLSLLSAMTFETMPPVEGGKMGVNHGFDEVRFKSPVK
                     TGSRIRARFVLASVKVRPSGWVQTAHDVTLEIEGEKTPALAARWLTLTFVEPQPA"
     gene            complement(9080..9304)
                     /locus_tag="VGM46_09340"
     CDS             complement(9080..9304)
                     /locus_tag="VGM46_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006206253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192834.1"
                     /translation="MAKSSTPASSAKASKTSAVRSFEKEQRDETAVTLEEELEVGLEG
                     TFPASDPVSVVSTSIPGKPKRREPKASGQK"
     gene            complement(9361..10035)
                     /locus_tag="VGM46_09345"
     CDS             complement(9361..10035)
                     /locus_tag="VGM46_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit gamma"
                     /protein_id="HEY4192835.1"
                     /translation="MVKPKTGVKPGTGVKPGTEAKQVTGAKQTARVTAAAKKAGSSTA
                     APSVERRSALAGKAVSAANVALAILPPAGRISVRAPEASLGSVSRALGLTLPQKPKTS
                     AAKDGRAALWLGPDEWLVLDEAGGDLVADCAKAKALHSAVDVSHRNVAISVTGTTAAA
                     TINSGCPQDLSLEVFPVGACSRTVLGKSEILLYRTAPDAFRVECWRSFSDYVFTYLTE
                     AARSAA"
     gene            complement(10028..>10612)
                     /locus_tag="VGM46_09350"
     CDS             complement(10028..>10612)
                     /locus_tag="VGM46_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HEY4192836.1"
                     /translation="RLFRMSFSGERGFEVNVPADYGEAVWEALWAEGQKHGACAYGTE
                     AMHVLRAEKGYIIVGQDTDGTVTVNDAGLDWAIGKKKTDFVGIRGMKRPDLVAKGRKQ
                     LVGLKTKDPKVVLEEGAQIVADPKQPIPMKMIGHVTSSYWSENCGRSIAMALVIDGRD
                     RMGETLYVPMPDGVIEVEVTGVVLFDEKGEHLNG"
BASE COUNT         1982 a         3518 c         3177 g         1935 t
ORIGIN      
        1 cggcgcatga cgccttcttc cttggtggtg atgccgatct ggtagtcgcc aatatagggc
       61 gaggcggtgg cgaggccttg ccagtgcttg ccgcgcgccg aatcataggt ttcgttgaac
      121 aggtagaatt cgagttcgga agcgaaatag ccgatatagc cgcgatcctg gagccgcttg
      181 acctgctttt tcaagatggc gcgcggcgaa tgcggcaggt cgtcatgcgt gtggtggtca
      241 agcacgtcgc agattacaag cgccgtcttt tccagccacg gaatgcggcg gatcgttgcg
      301 aggtccggct tcatgacgaa gtcgccatag cctttcgacc agctggccgc cttgtagccc
      361 ggcaccggct ccatatcgat gtcggccgcc agcagatagt tgcagccatg cgtctcgtca
      421 tgcgcggaat cgacgaaata atgcgccagg aaccgctttc cggcgagcct gccctgcatg
      481 tcgacgatgc aggccagcac cgtatcgatc tcgccattcg aaaccgcttt tttcaactga
      541 tcgaacgaga aattgccggc catcttcgga cgctccgctc atgaatgctg ggctgatgat
      601 actggaaaga aaagccgtgg caggtttgaa cccgccacgg ccttgtagcg ttagctgcgc
      661 tcacccaccg ctttttcggc agccgcgatc gcagcttgcc gggcggcaac gatctcgccg
      721 atgggcggcc cctggaagcg gcgtttctcg aaggcgaacc agatgatcgc cgtcaggatg
      781 aagaacccga ccgtgatgta gagcgcccaa tcattgggcg gctggatgcc gatgacgaag
      841 atcaggacca tcgagatcag cgacagtatt ccaaacagca tgaacacgcc cttacccatg
      901 ttccatggcc ccatcttgtc ccatttcggc gtcccccatg ccatcatgcc aagcacgatc
      961 ggcacggtga acgacaggaa caggaagatc acggtgcatg acaccacgat cgtataggcc
     1021 gaggtgccgg cgacagagac gagggtggaa ccccacacga acagcacggc cagcaccgcc
     1081 gccgtccaga tcgccgccac cggcgtgcgg taggtcgggc tgaccttgga aagcgcgccc
     1141 gatgccggca agccgccatc gcgcgagaag gcgaagatca tgcgcgacgc cgaggtcacc
     1201 gtggcaaggc cgcacaatag ctgcgccagg aagacgacga tatagacgat ctccttgacg
     1261 accggattga cctgctggtc gaacgcccag aagaacacgt tccaaccctg cttggcggcc
     1321 tcgtccatgt tcgggatcat cagcacgaaa gatgccagga acaggtagcc gaacagcgcc
     1381 gaccagatca ccgacatcac catggcgcgc ggcaccgaac ttgccgcctt gatggtttcc
     1441 tccgaagtat gcgccgaagc atcgtagccg gtgatcgtat agatcggaag caacaggccg
     1501 agcgcgaaca cccaggcgtt cgataccgca ggccagacat tgccgcccgc ctcaccggaa
     1561 taattgtgga atgtgaacag cctggcgaaa tcccacgtct gcgccgaaat caggcagacg
     1621 accgcgatca ggatcgcccc gaagaagatg aggtaaccgg aaaaatcggt cagctttgcc
     1681 gtcagcttga tgcccagatg attgatcagc gcctgcgcgc cggtgatgat gaccaggaag
     1741 atcatctggt tggtcagtgt gccttcgatg ccgagcgccg gaccgaatgc gccgacaaaa
     1801 aacgtccagg tgccgacatt gatggcgccg agcacagtga caaggccgag caggttgagc
     1861 catgccgtca cccagcccgt gccgcggttg ccgaggatcg aaccccagtg ataaaggccg
     1921 cccgccgttg gataggcgga gctgatctgc gacatggcga cggcgaaggt cagtgacacg
     1981 aagcagccga ccagccagcc gatgccgatg ccgatgccgc ccgcaccgga agtgccctgc
     2041 gccagcgaat tgatgccgcc ggacaggatg cagatgatgg aaaatgaaac cgcgaagttc
     2101 gaaaagcggc taagacgccg ctccagttcc tgggcgtagc ccatgctgtg gagaaccttc
     2161 acgtcctccg acttgtcgac atctgtatag tctcgcgctg ccatggtgac ctcccctttt
     2221 cggtttcagt tgaccgaccg tgccgagagc cccgcaggga gcgcccagtt acgttccatc
     2281 gcatcctctt ccatgcgtga aaaaataccg gcgagacgtt tcgcccattt ctgttgaccg
     2341 gccgcatccg aaatctcgtc attgcggatt tcgatcatgg cgcaggcaag tccgcgcggg
     2401 cgtgcgtgac gctccagcgt gaaatagacg aggtccgccg gcgaataggg ttcgttggcg
     2461 ccgacgacga tcccacccac accgcccagc gcacgcaaca gcggcgcggc aatgcgttcg
     2521 tcctcgtcat ggatgatgcc gatgtgccag ggccgcgcct tgccgagata gaccggcgtg
     2581 aacgaatgca tggaaacgat gcgcgtctca cgccccgccg ccaggcgcgc ttccaccgta
     2641 gcggcaatcg tctcgtgaaa aggctgccag gcaaggtcga tgcgggcctg ccgcagcgcc
     2701 aaggtcagcc gttcgttgcc gggaataacc gtgccttcgc tggtttccgc aatgaggttt
     2761 ggcgcgtcga gcggccggtt gcagtcgatg acgaggcgcg agacgcagct ttcgaccagc
     2821 gtggcgtcga gcatctgcgc catggttcgc gccacgggca aagccccggg atcccaggcg
     2881 atgtgccgct ccagttcagc cgccgccagc ccgagcgtgc cgaattcttc cggcacgaaa
     2941 ttggaagcgt ggtcgcaggt aaacagatag gggcttgaac caccggggtt cgtcaccctg
     3001 acggtgtcgg aaattgcaag gctgtctggc tctctcccca tcgccaacaa gtctccgtcg
     3061 gcgctgtacc atatggtact tattttatgt cattgcgcca cttcggatga tttcatacat
     3121 attgtgcgac tttgtcaaat gtgttctgga gatatgaaca cagcgctgtc gcaccgggat
     3181 gacatggcct cgaacatagc cgagctgatc tctgaacgaa tggccgccat gccggccggc
     3241 gagcgccgcg ccgcgcaaac gctggttgcc aactaccctt tgatcggcct gaaaaccgtc
     3301 gccgattttt cgcagcaggc gggcgtctcc tcgcccacca tcctgcgctt tgtcgcgagg
     3361 ctgggctttc ataactattc cgaattccag tccgcgctcc aggacgagct tgcggcccag
     3421 ctgcaatcgc ctgcctcgcg cacgcttgcg ccggccactg ccggcggagg tgaagtctcg
     3481 ccgctgcttg aggcgacgct cgacaatatg cgcgagacgt tccggcatct ctccgatgcc
     3541 cagcttggcg acatcgccgc caggctttgc gacctgcgcg gtcacgtcta tctggtcggc
     3601 ggccgcttta ccgatccgct ggcgcgctat atggccgccc accttgccat catccgcccc
     3661 aatgtcttcc acctcaccgg tcaggaaatc gcctggcgcg accggctgat cgacatgggc
     3721 aagcgcgatg tcctcatgat cttcgacatc aggcgctatc aggaaagcct gatccgtttt
     3781 tccgagaagg cgcatgagcg cggcgtgcag atcgtcttgt tcaccgacca gtggctgtcg
     3841 ccgatcgcgc gttttgcccg ccatgtgatt gccggacgca ccgctgttcc ctcggcgtgg
     3901 gattcatcgg cctcgctctt cgtggtgacc gaggcgctgg ttggcgcggt gacgcgccgg
     3961 ctcgaaaccg acaccgccag gcgcttgcgc gaaatggaag agttacgctg aaatcaacgg
     4021 gtaacgcctc gcccttccat gccaccttac atagaattaa tgtgcgctga cggcggaaaa
     4081 ttgtcataaa ggactgctat gggcgacgta attacgcgcc atggcatgcg gcttcgtcta
     4141 ctccggggag gggaaaccct gagagagcgg gtaagcgcgg ggaattcttt accaaaccgg
     4201 agtgccgatg gccgcctgga aacagatatt ttttgccctt gtggtggtgg tcgcggcagc
     4261 ggcagcctgg gtgcgctttt ttccgggcgc gccggcggtt ctcgaccgct ggggcatcga
     4321 atgggcccat gcttccatcg ccacgcccgg tgaggccacg gccgcgaagg ccggcggggc
     4381 aaaaagctcc atcccggtcg tcaccgccct tgtatccacc gccaccatca atggccgctt
     4441 gcaggcgatc ggttcgggcc gcgcaaaggc gaccgtcacc gtcaatcctt atgcggcggg
     4501 acgcctgacc gaattcctcg tccagtccgg ttctcatgtc gaaaagggac agaccgtcgc
     4561 ccgcctcgat tcggacaccg aaaccatcgc ggtcgaccgc gccaaggtcg gcgttcagga
     4621 cgcgcaatcc aagctcgatc gcgtcaagcg gctgcaagcc acccgaaccg catcgctcgt
     4681 ggccgtgtcg gatgccgaag tcacgctgga caatgccagg cttcagctgc gcgaagccga
     4741 actggcgctg gaacgccgct ccatcgttgc gcccatcggc ggcacggtcg gcatcctgcc
     4801 gatggaggcc ggcaattacg tcaccagtca gtcggccgtc gccaccatcg acgaccgcac
     4861 ggccatcctg gtcgatttct gggtgccgga acgcttcgcc gcctcgatca aggtcggcgc
     4921 gcccattacc gcgacgccca tcgccagccc gaaggacgtg ttccaggggt cggtggccgc
     4981 cgtcgacaac cgcgtcgacg agaaaagccg cacgctttgg gttcaggccc tgattgaaaa
     5041 cccggccgat tcgctgcgcg ccggcatggc attcgacgtc acgatgaagt ttccgggcga
     5101 cagctatccg gccgtcagcc cgctggcgat ccagtgggga accgacggcg cctacgtctg
     5161 ggccgtcgaa gacggcaaga ccaagcgcgt ggccgtccgc atcatccagc gtaataccga
     5221 aaccgtgctg atcgacgcgc cggtggtcag cggcgatata gtcgtgacgc agggggtcca
     5281 gagcgtgacc gagggcggcg aggtccgcat tgcgggcgaa gaccagcgcg cggcggacgc
     5341 cagcgggtcg tgaccgaagt tcataacaac gccaacgagg ccggctttac cgcgcttttc
     5401 gtgcgccggc ccgtcatggc tttggtgctg aacacgctga tcgcggtcgc cgggcttgcc
     5461 gccttctacg gcgtcgaaat ccgcgaattg ccggacgtgg accgcgccgt catctccgtc
     5521 accaccgatt tcgagggcgc ggccgccgag accgtcgacc gcgaactgac cgacaccatc
     5581 gaaggcaccg tcgcgcgcgt ctccggcgtg aagtcgattt cgtccacatc gtcattcggg
     5641 cgcagccgcg tcaccatcga attcaacgat ggcgtcgacc tgaacgtcgc cgcctcggac
     5701 gtccgcgatg cggtcggccg cgtcgccaac cagatgcccg acgaggccga cccgccgcgc
     5761 atcgtcaagg cggatgccaa ttccgatgcc gtgatgcggc ttgccgttac cgccgacaat
     5821 ttgtcggtcc aggacatgac ggtcatcgtg caggaccaga tcgaggatac gctggctgcc
     5881 gttcccggcg ttgccgacgt ccaggtctat ggcgaccgcg acaagatttt ccgcatcgac
     5941 gtcgaccaga acaagctggc gagccagggc ttcaccgttg cggatctgcg ggcggcactt
     6001 gcctccgtcg ccttcgattc cccggcgggt tcgatcaaca ccaccaacca gaacctcatc
     6061 gtgcgcacca cggcggacgt gacgacgccg gaggaatttg aaaacatcgt catcggcggc
     6121 aacacccata tccgcgacgt ggcgaccgtc acgctcggcc ccgatatcgg ccagacaacc
     6181 ctgcgctcgg acggcaagac gggcatcggc atcggcatcg tgcgccaggc cgaatcgaac
     6241 acgctggaca tttcaaacgg cgtgaaggca accgttgcca atttgcagaa aaccctgccc
     6301 aagggcatgt cgatcaagat caccagcgac gacgccgtct tcgtcaaggg cgccgtccac
     6361 gaagtggagg tggcgctcgg cctctcggtc accttcgtcc tgatcgtcat ctatcttttc
     6421 ctgctcgact ggcgcgctac ccttatcccc gctttgtcca tgccggtcgc catgatcggc
     6481 accatcgcgg cgatctatct tgccggcttc tcggtcaaca tcctgacgct gctggcactg
     6541 gtgctcgcaa ccggcctcgt cgtcgacgac gccatcgtcg tgctggaaaa catcgtgcga
     6601 cggcgcaacc agggcatggg gccacgcgcg gcggcggtac tcggcgccaa ggaagtgttc
     6661 ttcgccgtca tcgccaccac gctgaccctc gttgccgtgt tcgtgccgat ctcattcctg
     6721 cccggccaga ccggcgggct tttccgcgag ttcggcttcg ttctcgccat gtcggtgctg
     6781 ctttcttgcg tcgtcgccct cactttgtgc ccgatgctgg cttcacggct tctgaccaac
     6841 gcatccgccc cccaagaagt gcaaaagggt tttggcgccc gtgtcggcgg cctgctgaac
     6901 ggcctttacc gccgcgccct gcacatctgc ctcgatgcgc cgtggatcgt ggtcctggtg
     6961 gcgatattgt ttgcaggcac cgccttcgcg ctgttcggca gcatccgcca ggaattgacc
     7021 cccaccgaag accgctcggc ggttctgctg cgcatcaatg cgccgcaagg cgtcagcctc
     7081 gaatacacca ccgcgcagat ggaaaagatc gagcggctga tccagccgat acgcgattcc
     7141 ggcgagatcc gcagcacgtt cgaattcgcc ggcaacaacg gcgcctacaa taccggcttc
     7201 atggtgatga cgctggcccc gtgggaagag cgcacgcgca gccaacagca gatcatggcg
     7261 cagataagcg acctcgctag acaggtgccc agcattcgca tcttcccggt ccagccgaac
     7321 agcctaggca ttcgcggcgc cggcaacggc ctgcaattcg cccttgtcgg caacgaccgc
     7381 aaggccttgg gcgacgccgc caacaacatc atcgccgcga tgcagcagga cccgcgcttc
     7441 cagtcgccgc gcctgacggt cgacccgacg cagccgcagc tcgccgtggc gatcgaccgc
     7501 cagcgcgcct ccgacctcgg catcgacatt accggtcttg ccgatacctt ccaggccatg
     7561 ctcgacggca acgacgtggt cgacgtctac atcgccgacc gcagctatgg cgtgaagctt
     7621 gtttccacca ccaacccgat caacgacccg accgaccttg agaacatctt cctcaagacc
     7681 aaggatggcc gtttcgtgcc gatgtcgacc atcgccacgc tgacggaaaa ggcggtgccg
     7741 ccatcgctga cgcgcgaaca gcaacagcct tcggtcgcca ttacctccaa cctgcgctcg
     7801 gatttcgcgc tgggcgatgc cttcggcgtc gcgcaggtga ttgccgcccc cctgatgccg
     7861 caaggcgcgc gcatcctgcc gctggccgaa gccgccacgc ttggcgaaag caacagcgcc
     7921 atggtcacca tcttcggctt tgcgctggtc atcatcctgc tggtgctggc cgcgcagttc
     7981 gaaagcttcg tcagcgccgt catcatcatg gcgaccgtgc cgctcggcct tgcctgcgcc
     8041 atcgtcgcgc tgctgctcag cggcaccagc ctgaacgcct acagccagat cggcctggtg
     8101 ctgctggtcg gcgtcatggc caagaacggc attctgatcg tcgagttcgc caaccagatg
     8161 cgcgaccgcg gcctctcggt gcgcgaagcg atagagcagg ccgccaccat tcgcctgcgg
     8221 ccggtgatga tgaccatgat ctgtaccgtg ctgggcggcg tgccgctggt tctggcaagc
     8281 ggcgccggcg ccgaagcccg cgtggcgctt ggctgggtga ttgtcggcgg ccttggcctc
     8341 gccaccatct cgaccctgtt cctcacccca gtcgcctatc tgctgctcgg ccgtttcgtc
     8401 acgccgaaca tcgaggaaga ggcgcgcctg aaacgcgagc tggaggaagc cgcctatatc
     8461 gacatggagc cggcggaata gccgcgccac tccgttcttg atccgtcatg gatgacaacg
     8521 ccgccctcag gctctatctg tccggcagat gagagggcat ggatgccctg cctgactgga
     8581 gccaattgca tgcaaccgat cagcctcgac gatctcgtcg ccagcgccgg caaggaagtc
     8641 ggcgtttcgc cctggcgaat ggtgacgcaa accatgatcg accagttcgc cgacgccacc
     8701 gacgaccacc agttcatcca ttgcgacccc gagcgggcgg cgcgggagac accctatggc
     8761 ggcactatcg cgcatggctt cctgctgttg tcgctattgt cggcaatgac gtttgagacg
     8821 atgccgccgg tcgagggcgg caagatgggc gtcaatcacg gcttcgatga agtgcgcttc
     8881 aagtcgccgg tcaagaccgg ttcgcgcatc cgcgcccgtt tcgtgctggc cagcgtcaag
     8941 gtgcggcctt cgggctgggt ccagactgca cacgacgtga cgctggaaat cgagggagag
     9001 aaaacgccgg ctctcgccgc gcgctggctg acccttacat tcgtcgagcc gcagccggcc
     9061 tgaacctgaa cctgcggcgc tatttttgcc cgctggcctt tggctcgcgc cgcttgggct
     9121 tgccgggaat ggaagtcgaa acgaccgaaa ccggatcact ggccggaaac gtcccttcca
     9181 atccgacctc aagttcttct tcgagcgtca ccgcggtttc gtccctttgc tctttttcaa
     9241 aggagcggac cgccgaggtt ttcgaagcct ttgctgagga tgcgggggtc gacgatttgg
     9301 ccatgcttta tctctgtctc tgccgcattg aaccgacagc gggccaatgt ctgcgccgca
     9361 tcaggcggcg gagcgcgccg cctcggtgag ataagtaaaa acatagtccg agaacgaacg
     9421 ccagcactcg acgcggaaag catcgggcgc ggtgcggtaa agcaggatct cgctcttgcc
     9481 cagcacggtg cgcgaacagg ccccgaccgg aaagacctca agcgacaaat cctgcgggca
     9541 gccggagttg atcgtggcgg cagcagtcgt ccccgtcacg gaaatggcga cgttgcggtg
     9601 cgaaacatcg accgccgaat gcagcgcctt ggccttggcg caatcggcga ccagatcgcc
     9661 acccgcctcg tccagcacca gccattcgtc cggccccagc cacaaggcgg cgcggccatc
     9721 ctttgctgcg gaagtcttcg gcttctgcgg caaggtcagg ccgagcgccc gcgacacgga
     9781 accgagcgaa gcttccggcg cgcgcacgga aatgcgcccc gccggcggca atatggcgag
     9841 cgcgacattc gccgccgaga ccgccttgcc ggccagcgcc gaacggcgct cgaccgaagg
     9901 cgcggcggtg gaagaccctg ccttcttcgc cgcagccgta accctggccg tctgcttggc
     9961 cccggttacc tgcttggcct ccgttcctgg cttaaccccc gttcctggct taacccccgt
    10021 ttttggctta accattgagg tgctccccct tctcgtcgaa gagcaccacc ccggtgactt
    10081 ccacttcgat cacgccatcc ggcatcggca cgtaaagcgt ctcgcccatg cggtcgcgcc
    10141 cgtcgatcac cagcgccatc gcaatcgagc gcccgcaatt ttccgaccag tagctcgacg
    10201 tgacatggcc gatcatcttc atcgggatcg gctgcttcgg atcggcgacg atctgcgcgc
    10261 cttcctccag caccactttc gggtccttgg ttttcaggcc gaccagttgc ttgcggccct
    10321 tggcgacgag atcgggccgc ttcatgccgc gaatgccgac gaaatccgtc ttcttcttgc
    10381 cgatggccca atccagcccg gcatcgttga ccgtcaccgt gccgtccgta tcctggccga
    10441 cgatgatgta gcccttttcg gcacgcagca catgcatcgc ctcggtgccg taggcgcagg
    10501 cgccgtgctt ctggccttcc gcccacagcg cttcccaaac ggcctcgcca taatccgccg
    10561 gcacgttgac ctcaaagcca cgctcgcccg aaaacgacat gcgaaacagc cg
//
LOCUS       DASYCS010000296         1722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2833137,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000296 DASYCS010000000
VERSION     DASYCS010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2833137"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="VGM46_09355"
     CDS             complement(<1..514)
                     /locus_tag="VGM46_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEY4192837.1"
                     /translation="MAFGFIKKVFSFGKKETSGQQVDEAALQPNLDALKQLNPVAGQT
                     APDFLKCEDGALAELVQDGSASTPAPATEEPDPAEVPRPQPEPVEPEPETQPTPAPAE
                     IPDKPEPERPEPERPEPERPVPPPEEVPASPAQPAVPENIPAKVPQEVPTPAPEPAAP
                     EVVEVPAAPTP"
     gene            complement(527..>1722)
                     /gene="mtaB"
                     /locus_tag="VGM46_09360"
     CDS             complement(527..>1722)
                     /gene="mtaB"
                     /locus_tag="VGM46_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HEY4192838.1"
                     /translation="GAIIINTCAVTGEAVRQAKQSIRKARRENPQARIIVTGCAAQTA
                     PQDFSAMDEVDLVLGNEEKLHSHSYRALPDFGVNDFEKARVNDIFSVRETASHMVDAI
                     EGRARAFVQVQNGCDHRCTFCIIPYGRGNSRSVPMGGVVEQVKRLAGNGYAEVVLTGV
                     DMTSFGSDLPGAPKLGKLVKTILKQVPEVKRLRLSSIDSIEADDELLEAIATEPRLMP
                     HLHLSLQSGDDMILKRMKRRHLRDDSIRFCAEIRKIRPDIVFGADIIAGFPTETDEMF
                     ENSVRIVEECGLTHLHVFPFSPREGTPAARMPQVRREVVKSRAARLRAAGEAAYARHL
                     KSLTGSRQSILIERDGIGRTEGFTMASVGIGSPGEIVEATITGHDGARLLAAPLAAPL
                     AASAA"
BASE COUNT          278 a          560 c          550 g          334 t
ORIGIN      
        1 cgggcgttgg cgcggctgga acctcgacca cttccggcgc ggccggctcc ggggcaggag
       61 tgggaacttc ctgcggaacc ttggccggaa tgttttccgg gacggcaggc tgggccggac
      121 tggccgggac ttcctccggc ggaggcacgg gccgctccgg ttccggccgt tctggttcgg
      181 gccgttctgg ttccggcttg tcgggaattt ccgccggcgc gggcgtgggc tgcgtttcag
      241 gttcgggttc taccggctca ggctgtggcc gaggcacttc ggcggggtcc ggctcctccg
      301 ttgcaggcgc tggcgtcgaa gcagacccat cctgcacaag ctccgccagc gcgccgtctt
      361 cacacttcag gaagtccggc gccgtctgtc cagcaaccgg gttcagttgc ttcagcgcgt
      421 cgagattggg ctggagcgct gcctcgtcga cctgctgccc gctcgtctcc ttcttgccga
      481 aggaaaagac tttcttaatg aaaccaaacg ccattacgat ttcttttcag gccgcggacg
      541 cggccaaagg ggcagccagc ggcgcggcaa gcagccttgc gccgtcatgc ccggttatgg
      601 tcgcctcgac gatctcgccg ggagagccga tcccgacgct cgccatcgtg aaaccctcgg
      661 tgcggccgat gccatcgcgc tcgatcagga tcgactggcg cgaaccggtc agcgatttga
      721 gatgccgcgc ataggcggct tcgcccgcgg cgcgcaggcg cgcggcccgg ctcttgacca
      781 cctcgcgccg cacctgcggc atgcgcgcgg caggcgtgcc ttcacgcggc gaaaatggaa
      841 atacgtgcag atgcgtcagc ccgcattcct cgacaattcg caccgaattc tcgaacattt
      901 cgtccgtttc ggtcggaaat ccggcaataa tgtcggcgcc aaagacgata tcgggcctga
      961 ttttgcgaat ttctgcgcaa aaacggatcg aatcgtcgcg caaatggcgg cgtttcatcc
     1021 gtttcaggat catgtcgtca cccgattgca gcgacagatg caggtgcggc atcagccggg
     1081 gttcggtggc gatcgcctcc agaagctcgt catccgcttc gatggaatcg atggaggaaa
     1141 gcctgagccg cttcacctcc ggaacctgct tcaaaatggt tttcaccagt ttcccaagct
     1201 tcggcgcgcc cggcaggtcg ctgccgaaac tggtcatgtc cacacccgtc agcaccactt
     1261 ccgcatagcc gttgccggca aggcgcttga cctgctcgac cacgccgccc atcggcaccg
     1321 aacgggagtt gccgcgccca taggggatga tgcagaaggt gcagcgatgg tcgcagccgt
     1381 tctgcacctg cacgaaagcg cgcgcgcgcc cctcgatggc gtccaccatg tggcttgccg
     1441 tctcgcgcac cgaaaatatg tcgttgacgc gcgccttttc gaaatcattg acgccgaaat
     1501 ccggcagggc gcggtagctg tggctgtgca gcttttcctc gttgccaagc acgagatcga
     1561 cctcgtccat ggcggagaaa tcctgcggcg cggtctgcgc ggcgcaaccg gtgacgatga
     1621 tgcgcgcctg cgggttttcg cgccgcgcct tgcggatcga ctgcttggcc tgccgtaccg
     1681 cctcgccggt gacggcgcag gtgttgatga tgatggcccc gc
//
LOCUS       DASYCS010000297         3275 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1528930,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000297 DASYCS010000000
VERSION     DASYCS010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1528930"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..1009
                     /locus_tag="VGM46_09365"
     CDS             2..1009
                     /locus_tag="VGM46_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HEY4192839.1"
                     /translation="MAYEFPVFRFDGSDKSAPAAYLQAALHGGELPGTVAIDALMPKL
                     AKAEAEGRIRGAITVVPWANPVGRAQYHFGEHEGRFSLDTRGNFNRDFPLIDAPDVNL
                     LPSDQAGSLDRRLKTRLLKLSVGHDIVLDLHCDDEGVAYFYVPAQLWPSMQDCAAAMR
                     LEAVVVWEGDSGASFDEASLHPHLGSDKAKLSRLAVTNVEYRGILDVDGATAEADADG
                     LYRLLVARGVIEDAALPALPAFDGVVAPIENIEMIAAPKAGAILYDVKPGDRVAKGER
                     LATIVHAPCEAGGRTEVFAPQAGYVLTRRSRRIARTGEDLVKLVGERKSADARSGALE
                     D"
     gene            1333..>3275
                     /locus_tag="VGM46_09370"
     CDS             1333..>3275
                     /locus_tag="VGM46_09370"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006338618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase"
                     /protein_id="HEY4192840.1"
                     /translation="MTVQILENGAERFVTAGGVAITRQRHERPYEGAIDPYVDGLNSR
                     RGAVFSSNYEYPGRYTRWDTAIIDPPLVISARGRAMRIEALNARGEALLPVIGKALTP
                     LAELTITENSQKLIRLDVQKPGRVFSEEERSRAPSVFTVLRAVTGLFKSPEDANLGLY
                     GAFGYDLAFQFDPVEHTLERAQSQRDLVLFLPDEILVVDHYSAKAWTDRYDYSGDGFS
                     TEGLPRDQHSEPFKRSDRIPPRGDHVPGEYAKIVRKAMTSFKRGDLFEVVPGQMFYER
                     CESAPSDISRKLKSINPSPYSFFINLGEGEYLIGASPEMFVRVNGRRVETCPISGTIK
                     RGEDAIADHEQILKLLNSKKDESELTMCSDVDRNDKSRVCDPGSVRVIGRRQIEMYSR
                     LIHTVDHIEGRLREGMDAFDAFLSHAWAVTVTGAPKLWAMRFIEQNEKSPRAWYGGAI
                     GMVHFNGDLNTGLTLRTIRIKDGIAEVRAGATLLFDSIPEEEEAETELKASAMLSAIR
                     DAKAGNATSTERTAAHVGKGLNILLVDHEDSFVHTLANYFRQTGANVSTLRSPVPDEA
                     FDRLKPDLVVLSPGPGTPKDFDCAATIKKARARDLPIFGVCLGLQALAEAYGGELRQL
                     AVPMHGKPSRITVEKPGIIFSGLP"
BASE COUNT          610 a         1073 c         1004 g          588 t
ORIGIN      
        1 catagcctac gaattcccgg ttttccgctt cgacggcagc gacaagtcgg cgcccgccgc
       61 ctacctccag gccgcccttc atggcggcga attgcccggc accgtcgcca tcgacgcgct
      121 gatgcccaag ctggcgaagg ccgaagccga gggccgcatc cgtggcgcga tcaccgtcgt
      181 gccgtgggca aatcccgtcg gccgcgccca ataccatttc ggcgagcatg aaggccggtt
      241 cagcctcgat acgcgcggca atttcaaccg cgactttccg ctgatcgatg cgccggatgt
      301 caacctgctc ccgtccgacc aagccggatc gctggatcgt cgcctgaaaa cccgccttct
      361 caaattgtcg gtcggccacg acatcgtgct ggatctgcat tgcgacgacg agggcgtcgc
      421 ctatttctac gttccggccc agctgtggcc gtcgatgcag gattgcgccg ccgctatgcg
      481 gctggaagcc gtggtggtgt gggaaggcga tagcggcgct tccttcgacg aagcatcgct
      541 ccatccccat cttggctcgg acaaggcgaa gctcagccgc ctcgcggtca ccaacgtgga
      601 atatcgcggc attctcgatg tcgatggcgc gaccgccgaa gccgacgccg atggcctcta
      661 ccgcctgctg gtggcgcgcg gcgtgattga agatgccgca ttgccggccc ttccggcgtt
      721 cgacggcgtg gtcgcgccca tcgaaaacat cgagatgatc gccgccccga aggccggcgc
      781 gatcctctat gacgtcaagc ccggcgaccg cgttgcgaaa ggcgagcgcc ttgccaccat
      841 cgtgcacgcg ccatgcgagg ccggcggtcg caccgaggtt ttcgcgccgc aggccggcta
      901 tgtgctgacc cggcgctcgc gccgcatcgc ccgtaccggc gaggatctcg tgaaactggt
      961 cggcgaacgc aaaagcgccg atgcccgctc tggcgcgctg gaagactgac ctgctcgacg
     1021 ccggtgaatc cagttgcatg gcggcggttt cgccgctatg ggatttcaca tgaacatgca
     1081 ttcgaccaag aaggtttggt ggtgggcctg ctgacagcgg cctttcgaag cgcgtatgcg
     1141 tgaaaattga atggtggccg cagcggaata accgggcggc cattttattt tcatgctccg
     1201 ggtccgttgc caaggcatgt ctcccgaaaa tgggaaccgg tttcgggaca aagacctgcg
     1261 taaaggaact tgaggcggca taagcgcatc tgaaagatcg cgatacgctt cagggatcga
     1321 gtggagacgg caatgacggt gcagattctg gaaaatgggg cggagcgttt cgtgacagcc
     1381 ggtggcgtgg caatcacgcg ccagcgccat gaacggccct atgaaggcgc gatcgatccc
     1441 tatgtggacg ggctgaattc gcggcgcggc gcggtgtttt cctccaacta tgaatatccc
     1501 ggccgctata cgcgctggga caccgccatc atcgacccgc ctctggtgat ttcggcgcgc
     1561 ggccgcgcca tgcgcatcga ggcgctgaat gcgcgtggcg aggccctgct gccggtcatc
     1621 ggcaaggcgc tgacgccgct ggccgaactg acgatcacgg aaaactcgca aaaactgatc
     1681 cgcctcgacg tgcaaaagcc cggccgtgtc ttttccgagg aagaacgaag ccgcgcgccg
     1741 tcggtgttta ccgtgctgcg cgcggtaact ggcctgttca aaagccctga agacgccaat
     1801 ctcggcctct acggagcttt cggctacgac ctcgccttcc agttcgatcc ggtcgaacac
     1861 acgcttgagc gcgcgcagag ccagcgcgat ctggtgctgt tcctgcccga cgagattctg
     1921 gtcgtcgacc actattccgc caaggcctgg acagaccgct acgactattc cggcgacggc
     1981 ttctccaccg agggcctgcc ccgcgatcag cactccgagc cgttcaagcg gtcggaccgc
     2041 atcccgccgc gcggcgatca cgtccccggc gaatatgcaa agatcgtgcg caaggcgatg
     2101 acgagcttca aacgcggcga cctgtttgag gtcgtgcccg gccagatgtt ctacgagcgt
     2161 tgcgaaagcg cgccctccga catttcgcgc aagctgaaga gcatcaatcc ctcgccctat
     2221 tcgttcttca tcaatctggg cgaaggcgag tatctgattg gcgcgtcgcc cgaaatgttc
     2281 gtgcgcgtca atggccgccg cgtcgagacc tgcccgatct ccggcaccat caagcgcggc
     2341 gaggacgcca ttgccgacca cgagcaaatc ctcaagctgt tgaattccaa gaaggacgag
     2401 tccgaactca ccatgtgctc ggatgtcgac cgcaacgaca agagccgcgt ctgcgatccg
     2461 ggttcggtgc gcgtcatcgg gcggcggcag atcgaaatgt attcgcgcct catccacacc
     2521 gtcgatcaca tcgaagggcg gctgcgcgaa ggcatggatg ccttcgacgc attcctgtcg
     2581 cacgcctggg ccgtcaccgt taccggcgcg ccgaagctgt gggccatgcg cttcatcgag
     2641 cagaacgaga aaagcccgcg cgcctggtat ggcggtgcga tcggcatggt gcatttcaac
     2701 ggcgatctca ataccggcct gacgctgcga accatccgca ttaaggacgg catcgcagag
     2761 gtgcgcgccg gcgcaacgct gctgtttgat tccattcccg aagaagaaga agccgaaacc
     2821 gaactgaagg cctccgccat gctatccgcc atccgtgacg ccaaggccgg caacgccacc
     2881 tcgaccgagc gcaccgccgc ccatgtgggc aagggcctca atatcctgct ggtcgatcat
     2941 gaagacagct tcgtccacac gctggccaat tatttccgcc agaccggcgc caatgtctcg
     3001 acgttgcgca gccccgtgcc ggacgaggcg ttcgacaggc tgaagcccga cctcgtcgtg
     3061 ctgtcgcccg gccccggcac gccgaaggat ttcgattgcg ccgccaccat caagaaggcg
     3121 cgcgcccgcg atctgccgat cttcggcgtc tgccttggct tgcaggcgct ggccgaagcc
     3181 tatggcggcg agttgcgcca gttggcggtg cccatgcacg gcaagccgtc gcgcatcacc
     3241 gtcgaaaagc ccggcatcat cttttccggg ctgcc
//
LOCUS       DASYCS010000298         3406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2397621,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000298 DASYCS010000000
VERSION     DASYCS010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2397621"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="VGM46_09375"
     CDS             complement(<1..673)
                     /locus_tag="VGM46_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEY4192841.1"
                     /translation="MNPIRIDDPNDPRIAAYLDIQERDLTGRQGRFIAEGKVVLNVLL
                     SARRFRPQSILVLENRLAGLAGILAKAEPGLPVYVVAGEIMDQIAGFHMHRGILAIGA
                     RREAQTAEALLAELPANALAVILVGISNHDNIGSIFRNAAAFGADAVFLDETCCDPLY
                     RKAIRVSVGAALKVPFATFADTVAFVGALGQRGFDQFALSPNGACDIREARRGQRLAL
                     YLGTEG"
     gene            complement(815..1147)
                     /locus_tag="VGM46_09380"
     CDS             complement(815..1147)
                     /locus_tag="VGM46_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006338661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY4192842.1"
                     /translation="MNSTKLIDNAAAAAAFLMLMGNEKRLNIVSHLLEGEMSVGAIAD
                     KVSLSQSALSQHLAKLRALDLVQTRRDRQMIYYSCKSEMVRALMNMLEDVFGDQEPAV
                     PALRRTGT"
     gene            complement(1392..2165)
                     /locus_tag="VGM46_09385"
     CDS             complement(1392..2165)
                     /locus_tag="VGM46_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HugZ family protein"
                     /protein_id="HEY4192843.1"
                     /translation="MEDRKKDILRPTDAEAIRLARTLVRRARHGSLAFIDPDTGAPGV
                     NRVGVATDLDGAPLILVSMLSAHTPAILADPRCSLLVGVLGKGDPLAHPRITLACNAA
                     KIERGTPDHARAERRYLNRNPKAKLYAGLGDFSIFCLEPERASLNGGFGKAYLLNRAD
                     LLVAGPVNEELSVSEQGALDHMNADHLDAIAVYAHHFAKAAGDGWVLAGIDADGLDLA
                     SGDKSCRVFFPAPLKTTSELRPVLVEMAKAGRTAEQSPS"
     gene            complement(2242..3003)
                     /locus_tag="VGM46_09390"
     CDS             complement(2242..3003)
                     /locus_tag="VGM46_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008877897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEY4192844.1"
                     /translation="MNPNGLVAIVTGAGSGLGEATARALAGRGAKVAILDVGIERAEK
                     VASDIGGIAVKCDVSSAESGVAAMAEVAQKLGGARILVNCAGIAIGVKTVGKDGPHPL
                     DQYRRVIEINLIGTFNMIRLFADAAAKLDPLEGGERGVIVNTASVAAYDGQIGQAAYS
                     ASKGGVVGMTLPVARDLARSGIRICTIAPGIFKTPMMAGMPQEVQDSLGVAVPFPSRL
                     GEPSEYAALALHIVDNQMLNGETIRLDGAIRMAPK"
     gene            complement(3063..>3406)
                     /locus_tag="VGM46_09395"
     CDS             complement(3063..>3406)
                     /locus_tag="VGM46_09395"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEY4192845.1"
                     /translation="VIASVRMTPVAAGVGRAQMLGPLAVQPAFKNLGIGRRLVAIALE
                     QAAKAGVPAVLLVGDEPYYGPLGFTRVPHGQITMPRPVDLNRVLAHEIATGAVRRLSG
                     EVCHAAQAEAR"
BASE COUNT          638 a         1036 c         1110 g          622 t
ORIGIN      
        1 ggccttccgt gccgagatag agcgccagcc gctggcctcg cctggcttct cggatatcgc
       61 aggctccgtt cggggacagg gcgaattggt cgaaaccgcg ctggccgagt gcgccgacaa
      121 aggcgaccgt gtcggcgaaa gtcgcaaaag gcactttcag cgcggccccg acggaaacgc
      181 ggatcgcctt gcggtagagc gggtcgcagc aggtttcgtc gaggaagacg gcatcggccc
      241 cgaatgcagc cgcgttgcga aagatcgagc cgatattgtc gtgattggaa atgccgacca
      301 gtatgacggc gagggcattt gccggcagtt ccgccagtag cgcctcggcc gtttgcgctt
      361 cgcgcctcgc gccaatggcc aggatgccgc gatgcatgtg aaaaccggcg atctggtcca
      421 tgatctcgcc ggctacgaca tagaccggca ggccgggttc ggccttggcc aggatgcctg
      481 caaggccggc cagccgattt tcgagcacga ggatggattg cggcctgaag cgccgcgccg
      541 acaataaaac attgagcacg accttgcctt cggcaatgaa acggccctgc cggccggtca
      601 ggtcgcgttc ttgaatatcg aggtaggcgg cgatccgcgg atcgtttgga tcgtcgatgc
      661 ggataggatt catgatgccc ccggcaaacg ctttcgccgc aataaggcat tggaccggaa
      721 aagtggaatc cgttttcgga ttacccggcc gccggcacgg agcgccgcac ccaagaagcg
      781 cgatacgttt cgaaaaatgt tcggccgctg gcgctcaggt tccggtgcgg cgcaaggcgg
      841 ggaccgccgg ttcctgatcg ccgaagacgt cttccagcat attcatcagc gcgcgcacca
      901 tctcggactt gcaggaatag tagatcatct ggcgatccct gcgggtctgg accagatcaa
      961 gcgcgcgcag cttggccaga tgctgcgaga gggcggactg gctgagcgaa accttgtcgg
     1021 cgatggcgcc gacggacatc tcgccttcga gaagatggct tacaatattc aagcgcttct
     1081 cgttgcccat caacatcagg aaagccgccg cagcggcggc gttgtcgatc agctttgtcg
     1141 aattcattat tgcccacact tttctgccat tcgagcgcca tgggggcagc tggtgctcga
     1201 aatcctgcaa ttaattcaat gccgtctgga taagaatcga tttttggaag gtccaggcgg
     1261 cagttcaaaa caccacgata cttcgtgcgc gatactatga aacgcgccgc aaatcagcgg
     1321 acacgattcg cagcctaaaa catttggacc aaatctcaag agttgtgatc acagaaatgc
     1381 acacaatcgc gtcaggacgg cgattgttcc gctgtccggc cggccttggc catctcgacc
     1441 agaacgggcc gcaattccga agtggtcttc agcggcgccg ggaaaaatac ccggcagctt
     1501 ttgtcgccgg aagccagatc gagaccatcg gcgtctattc cggcaaggac ccagccatcg
     1561 ccggcggctt tggcgaagtg gtgcgcgtaa acggcgatcg cgtccagatg atcggcattc
     1621 atgtggtcaa gcgcgccttg ctcgctgacc gaaagctctt cattgaccgg cccggcgacc
     1681 aagaggtcag cgcggttaag caaataagcc ttgccgaagc cgccattgag gctggcgcgt
     1741 tcgggttcga ggcagaagat cgaaaaatcg ccgaggccgg catagagctt tgccttgggg
     1801 ttgcggttga ggtagcggcg ctcggcacga gcatggtccg gcgtgcctcg ttcaattttg
     1861 gcggcgttgc aggcaagggt gatgcgcgga tgcgccagtg gatcgccttt gccgagaacg
     1921 ccgaccagca gcgagcagcg cgggtcggcc aatattgccg gcgtatgcgc cgacagcatc
     1981 gagacgagga tcagcggcgc gccgtcgaga tcggtggcga caccgacgcg gttgacgcca
     2041 ggcgcgccgg tgtcgggatc gatgaaggcc agagagccat ggcgcgcgcg ccgcaccagc
     2101 gtcctggcca gccggatcgc ttcggcatcg gtcgggcgca gaatgtcttt cttccggtct
     2161 tccaatcttg ccaccgcgat aagctgatat tcacaatcca cttattgcgg gcgggccagc
     2221 gtttgacaag gctattttgg gctattttgg ggccatgcgg atggcgccgt cgaggcggat
     2281 cgtctcgccg ttcagcatct ggttgtcgac gatgtggagg gcaagcgccg catattcgct
     2341 gggctctccg agccgggagg ggaacggcac cgccacgccc agtgaatcct gcacctcctg
     2401 cggcatgccg gccatcatcg gcgtcttgaa gatgccgggc gcgatggtgc agatgcggat
     2461 gccggaccgg gcaaggtcgc gcgccaccgg cagcgtcatg ccgacgacgc cgcccttgga
     2521 ggcggaatag gcggcctggc cgatctggcc atcataggcg gcgacagagg cggtgttgac
     2581 gatgacgcca cgctcgccgc cttccagcgg gtcgagcttg gcggcagcgt cggcaaacag
     2641 gcggatcatg ttgaaggtgc cgatcaggtt gatctcgatg acccggcggt actggtcgag
     2701 cgggtgcggg ccatccttgc cgactgtctt gacgccgatg gcgatgccgg cgcagttgac
     2761 gaggatgcgc gcgccgccca gtttttgcgc cacttcagcc attgccgcga cgccgctttc
     2821 agcgctgctg acgtcgcatt tcacggcgat gccgccgatg tccgaggcga ctttttccgc
     2881 gcgctcgatg ccgacatcga gaatggcgac cttggcgccc cgtccggcca gcgcccgcgc
     2941 cgtcgcctcg cccaagcctg atccagcccc ggttacgatt gcgaccagtc cgttcgggtt
     3001 cattgccgtc tcctcatggt ttgtcgcaca gtaggcagcc gcagcgccgg aagcaacgcg
     3061 gcctatctgg cttcagcctg cgcggcgtgg caaacttcgc cggaaagcct gcgcaccgct
     3121 ccagtcgcga tttcatgcgc cagcacacgg ttgagatcga cggggcgcgg cattgtgatc
     3181 tgcccgtgcg gcacccgggt gaagccgagc gggccataat aaggctcgtc gccgaccagc
     3241 aagacagccg gcacgccggc tttcgccgcc tgttccagcg caatcgctac cagccgccta
     3301 ccgatgccga ggtttttgaa ggccggctgc acggccagcg ggccgagcat ctgggcgcgt
     3361 ccgacgccgg cggcaaccgg ggtcatgcgc acggaagcga tgacgg
//
LOCUS       DASYCS010000299         3254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1238482,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000299 DASYCS010000000
VERSION     DASYCS010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1238482"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VGM46_09400"
     CDS             <1..363
                     /locus_tag="VGM46_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002965374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HEY4192846.1"
                     /translation="ADARQLDRYAQRLGEVYAQPPVLRRKEVSETISGPLALLNAVFA
                     TGRRGLTMQRYKGLGEMNAEQLWETTLDPNVRSLLQVKVNDATGADSLFTRLMGDEVE
                     PRREFIQENALSVANLDI"
     gene            complement(513..1298)
                     /locus_tag="VGM46_09405"
     CDS             complement(513..1298)
                     /locus_tag="VGM46_09405"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY4192847.1"
                     /translation="MKKIAVLIALAAMLASACAHAQEPPLKMGPPLKMGVEGAYPPFN
                     FVDAAGKVEGFDVDIGEALCARLARKCEWIVVPWDGLIPALQAGKFDVLMSSMTITAE
                     RRKQVLFTKPYYFSYGMMIAARGSGLAFSAAGLKGRSIGVQAATTHEAWLRDKFGAAI
                     ELHAYPSSDDMFLDFQNGRLDAVFAEAPAMVPWMEKNGGTQAYEQIGPNITDPALGTE
                     IGIAVRKDDNAMAARLDAAMDGIVADRTFAAIAKKYFSFDLRP"
     gene            complement(1326..1895)
                     /gene="efp"
                     /locus_tag="VGM46_09410"
     CDS             complement(1326..1895)
                     /gene="efp"
                     /locus_tag="VGM46_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006336833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEY4192848.1"
                     /translation="MVKVIASSLRKGNVVDKDGKLYVILFAENIHPGKGTPVTQLDMR
                     RISDGVKVSERYRTTEQVERAYVEDREHTFLYSDGEGFHFMNPESYEQIAVSQDVIGD
                     QAPYLQEGMVVTLSVYESVGIAIVLPQRAVYEVVDTEPTTKGQTASSSYKPAILSNGV
                     KTTVPPHIGVGTRVVVMTADGAYVERAKD"
     gene            2009..3064
                     /gene="epmA"
                     /locus_tag="VGM46_09415"
     CDS             2009..3064
                     /gene="epmA"
                     /locus_tag="VGM46_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018262833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HEY4192849.1"
                     /translation="MTNASPWWTPHVHADRRPRLMLRNRIATATRDFFATRDFIEVTV
                     AALQISPGNEAHLSAYATAEVRPDGTTAPLYLHTSPEFACKKLLAAGERRIFSMGPVW
                     RNRERGPLHHPEFTMLEWYRVGETYESLMADCAGLLALAAERAGTKRFIFRNREADPF
                     AEPERLTVAEAFMRHAGIDLLATIAPDGGPDRLALHGALVQAGLRTAPDDTWSDLYSR
                     VMVECVEPALGMGRATVLCEYPIVEAALARPAAHDPRVAERFELYCCGVELANGFGEL
                     TDAAEQRRRFTAEMDEKERVYGERYPIDEDFLAALAIMPEASGVALGFDRLAMLAAGA
                     NKIEDVIWTPVADRATP"
BASE COUNT          579 a         1012 c         1035 g          628 t
ORIGIN      
        1 gccgatgcac gccagctgga ccgttatgcg cagcgcctgg gcgaggttta cgcccagccg
       61 ccggttctgc gccgcaagga agtgtccgag actatctcgg ggccgctggc gctgctcaat
      121 gctgtcttcg ccacgggccg caggggcctg accatgcagc gctacaaggg ccttggcgaa
      181 atgaacgccg agcagctttg ggaaacgacg cttgacccga atgtacgctc gctgcttcag
      241 gtcaaggtca atgacgcaac gggcgcggat tcgctgttca cccggttgat gggcgacgag
      301 gttgagccgc ggcgtgagtt catccaggaa aatgcgctgt cggtcgccaa tctcgacatt
      361 tgatctggca gccatgtcgg cagcctgaag gctggccggc tttgcagaac tgaaaacggc
      421 cgcgacggct ttcgtcgcgg ccgttctatt tggggcggag cgcctgacag gcgcggcgga
      481 aagccgatgc tgcctgcatc aggcgcaggc cctcaagggc gcaggtcgaa gctgaaatat
      541 ttcttcgcga tggcggcgaa agtgcggtcg gcaacgatgc cgtccatcgc cgcgtcaagc
      601 ctggcggcca tggcgttgtc gtccttgcga acggcaatgc ctatttcggt gccgagagcg
      661 ggatcggtta tgttcggccc gatctgttca taggcctgcg tgccgccgtt cttttccatc
      721 cacggcacca tggctggcgc ttcggcaaac accgcatcca gccggccatt ctggaaatcg
      781 aggaacatgt cgtcgcttga aggataagcg tgaagctcga tggccgcgcc gaacttgtcg
      841 cgcagccatg cctcgtgcgt cgtggccgcc tgaacgccga tagagcggcc cttcaggccc
      901 gcggcggaga aggcgaggcc gcttcccctg gcggcgatca tcatgccgta ggagaagtaa
      961 tagggcttgg tgaagagcac ctgcttgcgg cgctcggcgg tgatggtcat cgacgacatc
     1021 agcacatcga acttgccggc ctgaagcgcc gggataaggc cgtcccacgg caccacgatc
     1081 cattcgcatt tgcgcgccag gcgcgcgcac agcgcctcgc ctatatcgac gtcgaaacct
     1141 tccaccttgc cggcggcatc gacaaagttg aacggcgggt aagcgccttc cacgcccatt
     1201 ttcaggggcg gccccatttt caagggcggc tcttgcgcat gggcgcaagc gctggccagc
     1261 atggcggcga gcgcgatgag gacggctatc tttttcactt aagcgatcct gccggccctg
     1321 aagggtcagt ccttggcgcg ctcgacatag gcgccatcgg cggtcatgac gacgacgcgg
     1381 gtgccgacgc cgatatgcgg cggaacggtg gtcttgacgc cgttggaaag gattgccggc
     1441 ttgtaggatg acgaggcggt ctggcccttg gtggtcggct cggtatcgac aacttcatag
     1501 acggcgcgct gcggcaggac gatggcgatg ccgacgcttt catataccga aagcgtcacc
     1561 accatgcctt cctgtaaata cggcgcctga tcgccgatca catcttgtga aaccgcaatc
     1621 tgctcgtagc tttccgggtt catgaagtgg aagccctcgc catcgctgta gaggaaagtg
     1681 tgctcgcggt cctcgacata ggcgcgctcg acctgttcgg tcgtgcggta gcgctccgac
     1741 accttcacgc catcggaaat gcggcgcatg tcgagctgcg tgaccggcgt gcccttgcca
     1801 ggatggatat tctcggcaaa taggatcaca taaagcttgc cgtccttgtc gacgacgttg
     1861 cctttgcgaa gcgaactggc gatcaccttc accatgataa atcctaacgg tactgttgag
     1921 cctgacgcgg catcgaatgc cttatgctgg gccgtagcgc atctttcagg ctaccgccag
     1981 cctttcacgc gattttaggg cgatagcgat gacgaatgcc tccccctggt ggacgccgca
     2041 cgtccatgcc gaccgccgcc cgcgcctgat gttgcgcaac cgcatcgcta cggcgacacg
     2101 cgattttttc gccacgcgcg acttcatcga agtaacggtg gccgccttgc agatttctcc
     2161 cggcaacgag gcccatctca gcgcctatgc caccgcggaa gtgcggccgg atgggacgac
     2221 cgcgccgctt tacctgcaca cctcgcccga attcgcctgc aagaagctgc ttgcggcggg
     2281 tgaacgacgc attttttcga tggggccagt gtggcgcaac cgcgagcgcg ggccgctgca
     2341 ccatcctgaa ttcaccatgc tggaatggta tcgggtcggc gaaacctatg agagcctgat
     2401 ggccgattgc gccgggcttc tggcgctggc cgccgaacgc gccggcacga aacgcttcat
     2461 cttcaggaac cgtgaagccg atccgtttgc cgagccggaa cggctgaccg tggccgaggc
     2521 cttcatgcgc cacgccggca tcgatctgtt ggcgaccatc gcgcccgatg gcggtcccga
     2581 ccgccttgcc cttcacggcg cgctggtgca ggcaggcttg cgcaccgcgc ccgacgacac
     2641 atggtccgac ctttatagcc gcgtcatggt ggaatgcgtc gagccggcgc ttggcatggg
     2701 ccgcgccacc gtcttgtgcg aatatccgat cgtcgaggcg gcccttgcgc ggccggcggc
     2761 gcacgatccg cgcgtggccg aacgcttcga gctttactgt tgcggcgtcg agcttgccaa
     2821 cggctttggc gaactgaccg acgccgccga gcagcgccgc cgcttcacgg cggaaatgga
     2881 cgagaaagag cgcgtctatg gcgagcgcta ccccatcgac gaggatttcc tggcggcgct
     2941 cgccatcatg ccggaggcaa gcggcgtggc gctgggcttc gaccggctgg cgatgctcgc
     3001 cgccggcgcc aacaagatcg aagacgtgat ctggacgccg gtggcggaca gggcaacccc
     3061 gtaaaggcaa ttcatggtgg cggtggccac gcgctgccga tgacaattcc cgtcgcggaa
     3121 aggctgcgac ttaacctctc gcggaactga agtcgcgtcg gcgcattatg ctgatgttac
     3181 tggcttctac aggaggtttt catgggtcgc ggcattctac tctggttgct tggcattcct
     3241 attccgatca tcat
//
LOCUS       DASYCS010000300         4156 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1821203,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000300 DASYCS010000000
VERSION     DASYCS010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1821203"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="VGM46_09420"
     CDS             complement(<1..350)
                     /locus_tag="VGM46_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010972222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HEY4192850.1"
                     /translation="MLDFAIFWEWLSFGVRWLHVITAIAWIGSSFYFVALDLGLRQRP
                     GLPAGAYGEEWQVHGGGFYHIQKYLVAPAEMPEHLTWFKWESYATLLSGLALLAIVYY
                     AGANLFLIDPNVLDV"
     gene            496..1416
                     /locus_tag="VGM46_09425"
     CDS             496..1416
                     /locus_tag="VGM46_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006325385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY4192851.1"
                     /translation="MAYLDNIAVFVRVVELGNLSAAGRDMRISPAVASNRIKELEKHL
                     GVRLFNRTTRQLMPTEHGTVFYTGAKQVLDSISEAEAAVSALSGQPRGTIKVTAPLGL
                     GRRLIASGIPDFRDRYPDIEVRLRLSDHNVDIMKEGIDVAFRLGVIEDSSLRMRGIME
                     CERVLVAAPSYLETHGEPTEPGELLGKKHDCLMLRYPGSREFYWMLQTPAGPQKFEVH
                     GPFDSDDGDVLTGWALSGRGIINKPRFEVEPYLREGRLKVILAATPPTPVQFAAVYPH
                     KKLQDPKVRLLLDFMAERCHRLIKELLAGE"
     gene            1514..2002
                     /gene="dps"
                     /locus_tag="VGM46_09430"
                     /gene_synonym="pexB"
     CDS             1514..2002
                     /gene="dps"
                     /locus_tag="VGM46_09430"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018429452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HEY4192852.1"
                     /translation="MANHPTHNDLKSNTKKVSIDILNARLADAIDLALITKQAHWNVK
                     GPQFIAIHEMLDGFRTELDVHVDTIAERVVQLGGTAIGTTQAVAKATPLKPYPTDIYK
                     TKDHLAALIERYALAAKNARTAIDDTDEAGDADATDIFTAYSRALDKALWFLEAHTQE
                     KE"
     gene            complement(2012..2905)
                     /gene="xdhC"
                     /locus_tag="VGM46_09435"
     CDS             complement(2012..2905)
                     /gene="xdhC"
                     /locus_tag="VGM46_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018429453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory protein XdhC"
                     /protein_id="HEY4192853.1"
                     /translation="MTLKPDALKDFLAGSGRFAIVEVARTRGSTPRETGTIMLVSPTG
                     THGTIGGGQLEYMAIDKARQMLSPRARSGNNATSHIEAEEVCATLDIPLGPEIGQCCG
                     GRVEVLIRMGTPVITEELLRKAEGEEVRRPHLYIFGGGHVGHAMAASCALMPVRTIVV
                     DTRAEAFDGLTQGIETRLAAMPEQLVREAPAGSAFAVLTHDHALDFLIVAEALRRTDA
                     SYVGMIGSGTKKATFRSWFLKTAAGTEAEFARLVSPIGGETVKDKRPAVIAALAAAEI
                     MTALAHCSSGIGACDRPRVAG"
     gene            complement(2973..>4156)
                     /locus_tag="VGM46_09440"
     CDS             complement(2973..>4156)
                     /locus_tag="VGM46_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016555785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY4192854.1"
                     /translation="FYDPMGKEAGRNVTPYHQKVEDCIIQRIVAELEESADYAGRRRE
                     ISAFNANSRFIKRGLALTPVKFGISFTKTESNQAGALVHVYTDGSVHMNHGGTEMGQG
                     LHMKVAQVVAEEFQIDIDRVKITATTTAKVPNTSPTAASSGADLNGMAAQNAARQIKD
                     RLIDFAAETHQVAKEQVVFLPNRVRVGNQEISFNDLVRQAYMARIQLSAAGFYKTPKI
                     HWDRAKGRGHAFYYYAYGAACSEVSVDTLTGEYVVERTDILHDAGRSLNPAIDLGQVE
                     GGFIQGMGWLTTEELWWDGKGRLRTHAPSTYKIPLASDRPKIFNVALTDWSQAYEPTV
                     HRSKAVGEPPLPLGLSVLHALSDAVASVADHKVCPRLDAPATPECVLMAIERLKRDRA
                     S"
BASE COUNT          814 a         1325 c         1283 g          734 t
ORIGIN      
        1 acgtcgagaa cgttgggatc gatgaggaag aggttcgcgc cggcgtaata gacgatggcg
       61 agcagggcca ggcccgacag aagcgtggcg tagctctccc atttgaacca tgtcaggtgt
      121 tcgggcattt cggcgggcgc caccagatat ttctggatat ggtagaagcc gccgccatgc
      181 acctgccatt cctcgccata ggcgccggcc ggcaggccgg ggcgctggcg caggccaaga
      241 tcgagcgcga cgaaataaaa ggacgagccg atccacgcaa tggcggtgat gacgtgcagc
      301 cagcgcacgc caaagctcag ccactcccag aaaatcgcga aatcgagcat gatcgtcctt
      361 ccaccctgcg caacgacttt acgggctggc gcgcaaaccg aaaggggcgg cgcgcacgat
      421 gactttcaaa aaaaattgga aaatactagg ctgttcccac gcagccgcat taacagccac
      481 gaatcgaatg ccgccatggc ctatctcgac aacatcgccg tctttgtccg cgtcgtcgaa
      541 ttgggcaacc tctcggcggc ggggcgcgac atgcgcattt cgccggcggt cgcctccaac
      601 cgcatcaagg aactggaaaa acaccttggc gtgcgcctat tcaaccgcac cacgcggcaa
      661 ttgatgccaa ccgagcacgg cacggtattc tacaccgggg cgaagcaagt gctcgattcc
      721 atttcggaag cggaagcagc cgtcagcgcg ctttccggcc agccgcgcgg caccatcaag
      781 gtaacggcgc cgcttgggct ggggcgccgt ctgatcgcct cgggcattcc cgattttcgc
      841 gaccgctatc cagacatcga ggtgcggctt cggctttccg accacaatgt cgacatcatg
      901 aaggagggca tcgacgtcgc cttccggctc ggcgtcatcg aggattcatc cctgcgcatg
      961 cgcggcatca tggaatgcga gcgcgtgctg gtggccgcgc caagctatct ggagacgcat
     1021 ggcgaaccga ccgaacccgg cgaactgctc ggcaagaagc acgattgcct gatgctgcgc
     1081 tatcccggca gccgggaatt ctactggatg ctgcaaacgc cggccggccc gcagaaattc
     1141 gaggtacacg ggccgttcga cagcgacgat ggcgacgtgc tgaccggctg ggccttgtcg
     1201 gggcgaggca tcatcaacaa gccacggttc gaggtcgaac cctatctgcg cgaagggcgg
     1261 ctgaaagtga tcctggccgc tacgccgccg acgccggtgc agttcgccgc cgtctatccg
     1321 cacaagaaat tgcaggaccc gaaggtgcgc ctgctgctgg acttcatggc cgagcgctgc
     1381 caccggctga tcaaggaact gcttgcgggc gaatagacgc aaggccttga gcaaccgcgt
     1441 catacatcgc acactggccg gaggaagggg cgcaacgacg cctgcgacct cactacaatc
     1501 catggagttc ccgatggcca accacccgac gcacaacgac ctgaaatcga acacaaaaaa
     1561 ggtctcgata gacattctca acgccaggct cgccgatgcg atcgatctgg ccctgatcac
     1621 caagcaggcg cattggaacg tcaagggacc gcagttcatc gccatccacg aaatgctgga
     1681 cggttttcga accgaacttg acgttcatgt cgataccatc gccgaacggg tggtgcagct
     1741 tggcgggacc gccatcggaa cgacgcaggc cgttgccaag gcgacgccgc taaagccgta
     1801 tccgaccgat atctacaaga ccaaggacca ccttgccgcc ctgatcgagc gctatgcgct
     1861 ggcggccaag aacgcgcgca ccgcaatcga cgatacggac gaggcgggcg atgccgacgc
     1921 gaccgacatt ttcaccgcct attcgcgcgc gctcgacaag gcgctctggt tcctcgaagc
     1981 ccatacgcag gaaaaggaat agtcgttcgg atcagccggc cacgcggggc cggtcgcagg
     2041 ccccgatacc ggatgagcaa tgggcaagcg ccgtcatgat ctcggccgcc gcaagggccg
     2101 cgatcacggc cgggcgcttg tcctttaccg tctcgccgcc gatgggcgag accagccgcg
     2161 caaattccgc ttccgtgccg gccgccgtct tgaggaacca gctgcggaac gtggctttct
     2221 tggtgccgga accgatcatg ccgacatagc ttgcgtcggt gcggcgcagc gcttccgcca
     2281 cgatcaggaa atccagcgcg tggtcgtggg tgagcacggc gaaggccgaa ccggcaggcg
     2341 cttcgcgaac caattgctcc ggcattgccg ccagcctggt ttcgatgcct tgcgtcaaac
     2401 cgtcgaacgc ctcggcgcgc gtgtcgacca cgatcgtgcg gaccggcatc agggcgcagg
     2461 atgcggccat ggcgtggccg acatggccgc cgccgaaaat gtagagatgc ggccgccgca
     2521 cttcctcgcc ctcagctttc ctgagcagtt cttcggtaat caccggcgtt cccatgcgga
     2581 tcagcacctc gaccctgccg ccacaacact ggccgatctc cgggccaagc ggaatatcga
     2641 gcgtggcgca aacctcctct gcctcgatat gcgacgtggc attattcccg gacctggcgc
     2701 gcggcgacag catctgccgc gccttgtcga tggccatata ttcaagctgg ccgccgccaa
     2761 tggtgccatg cgttcctgtt ggcgagacaa gcatgatggt gccggtttca cgcggcgtcg
     2821 aacccctggt cctggccact tcgacaattg cgaagcggcc ggagccggcg agaaagtcct
     2881 tcaaggcatc aggcttcaag gtcatggctc gtatttccgt cccggaaata taggtctttt
     2941 cccgaataac cgccattttt cgccgatgaa ggtcaggaag cccggtctct cttcagccgt
     3001 tcgatggcca tcaacacgca ttcgggcgta gcgggcgcat cgagacgcgg gcagaccttg
     3061 tggtcggcga cactggccac cgcatccgac agcgcgtgca gcaccgaaag accgagcggc
     3121 agcggcggct cgccaaccgc tttcgagcga tgcacggtcg gctcgtaggc ctgcgaccag
     3181 tccgtcagcg cgacgttgaa gattttcggc cggtccgagg cgagcggtat cttgtaggtg
     3241 gaaggcgcgt gcgtgcgcag gcggcccttg ccgtcccacc acaattcctc ggtcgtcagc
     3301 cagcccatgc cctggatgaa accgccctcg acctggccga gatcgatggc cgggttcagc
     3361 gagcggccgg catcgtgcag gatatcggtg cgttcgacca catattcgcc ggtcagcgta
     3421 tcgaccgaaa cttccgaaca ggccgcgcca taggcgtagt aatagaaggc gtggcccctg
     3481 cccttggcgc ggtcccagtg aattttcggc gtcttgtaga agcccgccgc cgaaagctgg
     3541 atgcgcgcca tataggcttg ccggacgagg tcgttgaagc tgatttcctg gttgccgacg
     3601 cgcacacggt tgggcaggaa caccacctgt tccttcgcaa cctgatgcgt ttcggcggcg
     3661 aaatcgatca gccggtcctt gatctggcgc gccgcgttct gcgccgccat gccgttgaga
     3721 tcggcccccg aagaagccgc cgtcggcgag gtgttcggca ctttggcggt ggtggtggcg
     3781 gtgatcttca cccggtcgat gtcgatctgg aattcctcgg ccaccacctg cgccaccttc
     3841 atgtgcaggc cctgccccat ctcggtgccg ccatggttca tgtgcaccga gccgtcggtg
     3901 tagacgtgca ccagagcgcc ggcctggttg gactcggtct tggtgaagga aatgccgaac
     3961 ttgaccgggg taagcgccag cccgcgcttg atgaaacggc tgttggcgtt gaaggcggag
     4021 atctcgcgcc gccggccggc ataatcggcg ctttcctcaa gctcggccac gatgcgctgg
     4081 atgatgcagt cctcgacctt ctgatggtag ggcgtgacgt tgcggccagc ctccttgccc
     4141 atcgggtcgt agaaat
//
LOCUS       DASYCS010000301         8507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1678946,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000301 DASYCS010000000
VERSION     DASYCS010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 8507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8507
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1678946"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /locus_tag="VGM46_09445"
     CDS             complement(<1..926)
                     /locus_tag="VGM46_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008875975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEY4192855.1"
                     /translation="MTDQKPPRGAEGGPTGIEPISIIEEMQRSYLDYAMSVIVSRALP
                     DVRDGLKPVHRRILYAAHESGYHWNRKYVKSARPVADVMGKYHPHGDASIYDALVRMA
                     QDWSLRVPLVDGQGNFGSIDGDPPAAMRYTESRLTKVAHELLEDIDKETVDFQDTYDA
                     SGSEPKVLPARFPNLLVNGSGGIAVGMATNIPPHNLGEVCNGAIALIDNPAIDLPELM
                     EIIPGPDFPTGAIVLGRSGIYSAYATGRGSIVMRGRASVEPRGNDRESIVITEVPYQV
                     NKATMIEKMAELVRDKRIEGISDIRDESDRQG"
     gene            1171..1800
                     /locus_tag="VGM46_09450"
     CDS             1171..1800
                     /locus_tag="VGM46_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEY4192856.1"
                     /translation="MPSFDNIFNAFVTILVTIDPPGLAPLFLAVTRGMNRDERRQVSI
                     RASIIGFAVMALFAVAGASILSIFGITLPAFRVAGGFLLFFIAFEMVFERRQDRKEKI
                     SDVAITKDMIHNIAAFPLAIPLIAGPGAISATVLLSGSFQGFAAQATLVGIILACLVL
                     TYLVFVLSERIDRFLGQTGRSILTRLLGVILAALAVQFVADGVKALVAG"
     gene            complement(1803..2288)
                     /locus_tag="VGM46_09455"
     CDS             complement(1803..2288)
                     /locus_tag="VGM46_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEY4192857.1"
                     /translation="MTRTPPTYEQLRTMEGQELGVSDWMEVDQARIDQFAACTGDRQW
                     IHTDPVRAKRQSPFRTTIAHGYLTLSIIGCLSQEMGVIPENTQAAFNYGLDRVRFLSP
                     VKVGSRVRLRVTLLSMEDKGPGQYLMKVANTIEIEGEEKPALMAETLAMFYERRNRKA
                     G"
     gene            complement(2402..2833)
                     /gene="phaP"
                     /locus_tag="VGM46_09460"
     CDS             complement(2402..2833)
                     /gene="phaP"
                     /locus_tag="VGM46_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836825.1"
                     /note="Members of this family are phasins (small proteins
                     associated with inclusions such as PHA granules). Note
                     that several different families of phasins have been named
                     PhaP despite very little sequence similarity to each
                     other; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01841 family phasin"
                     /protein_id="HEY4192858.1"
                     /translation="MAKKPEEPSFMDMFARFGRDLKLPNLDVDAIIEHQRRNFDALEK
                     SARVTATGTAALMRKQRDMLEDTLRQTSEMAKHYKTPGNPEELIARHAEFARKAFESA
                     VSNAGEVAELVKSSGTESIEILRNRIKEAMVEVREEYEKKK"
     gene            complement(2899..4185)
                     /locus_tag="VGM46_09465"
     CDS             complement(2899..4185)
                     /locus_tag="VGM46_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HEY4192859.1"
                     /translation="MTQAGFSPIDPDSEQSAKLSALRRTKFMASAALVLCVVVFIAAH
                     SFQGLYPGLGFVAACAEAAVIGGLADWYAVVALFRRPLGLPIPHTAIIPANQNRIAEN
                     LGRFIEVNFLAPGPVREKLDEVDFAALVADWLSDPGRAAGLSRFVARLVPQTLDAVEQ
                     SGLRGFVSSRMLEQAEKVQLAPLAAELLSAFTDDRRHQKLFDEFTRVIGRFLSDEQAL
                     AVMREKIREELPSLFNLFRADAYLLKKIVASAASLLEEVRAEPDHPLRAEFDGFVAAF
                     IERLKTSKDYARRAEKLKRDFLARPELRDMAQAMWTSLRQFIEKDAQSDESVIRAHLA
                     GMFVEIGRHLTGDASVRADMNKGFVVALSAFVESQKSGVSTFIADQVKRWDLAQLTRL
                     IEINIGRDLQYIRFNGMIIGGLAGLALYTVQLLLAN"
     gene            complement(4224..5441)
                     /locus_tag="VGM46_09470"
     CDS             complement(4224..5441)
                     /locus_tag="VGM46_09470"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEY4192860.1"
                     /translation="MRTQSVAMTQPAYAGSRGETASASQVRFLVLDSWRGVCALLVAL
                     LHFPTSSAISQSAFVGGAYLFVDFFFVLSGFVIASSYGGRLDEPKKVARFALVRFGRL
                     YPLHLFMLLAFVAFELLRLMLPQLRGAGAAPFTEDNSIGSLVANLLLLQGTGLEDRLT
                     WNGPSWSISTEFFAYLFFAAVVFVARKRAWAVLACAAVVGPLVLLRFADTMDVSYDFG
                     LVRCLYGFSIGALLSWFQHDRLVEARRDAGQGTDRLIWTLLELAMIAVIALFVVDFSD
                     NKGGIAAPLLFAVALYVFAHEGGWVSSLLRTRPMLTLGALSYSIYMVHIFVQGRMINV
                     AGLVDRKFGLGLVGPLSLRGDTAIGIGPDAPWTGLAAIALMVAATLVASWFTWRFVEM
                     PALALFRRLAERI"
     gene            5640..6638
                     /gene="speB"
                     /locus_tag="VGM46_09475"
     CDS             5640..6638
                     /gene="speB"
                     /locus_tag="VGM46_09475"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEY4192861.1"
                     /translation="MPNSTIDHAFNARAMTGASLEPTYAGALSFMRRKYTKNVKGADA
                     VVWGIPFDAAVTNRPGARFGPQAIRRAAAILDNDPQYPFARDIFEHLAVVDYGDCLLD
                     SGNHQKTPATIEREAAKLLKSGAFLLTLGGDHFVTWPVLKAHAEIHGPLALVQFDAHQ
                     DTWPDDGKRIDHGSFVGRAVKEGIIDPDRSIQIGIRTHAPDTFGIKILYGHEIEEMRA
                     SDIAYAIVERTGGKKTYVTFDIDCLDPAFAPGTGTPVAGGPSSAKILSTLRQLGQIDI
                     VGADIVEVAPAYDHADITAIAGSTIAMQYLGLLAERKARLSETENSAAAMNPGSGI"
     gene            6676..7608
                     /gene="argC"
                     /locus_tag="VGM46_09480"
     CDS             6676..7608
                     /gene="argC"
                     /locus_tag="VGM46_09480"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018427391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEY4192862.1"
                     /translation="MKPKIFIDGEHGTTGLQIRALLADRGDLEILSIPTGRRKETAAR
                     AEYLNAADVAILCLPDDAARESVSLIANDKTKVIDASTAHRVAEGWEYGFAELDKDQP
                     QRIASAKRVANPGCWPQGPIASLRPLIDAGLLPADYPVTVNGVTGYSGGGRPMIEDYV
                     AKGEDAPEFLPYGLTLHHKHVPELKTYSRLLRDPIMQPAVGNFAQGMLTVVPLQLGNL
                     DYVPSGAELHAAIGDHFAGIAGSVVEVAPFAQVERMAEIDPEHLNGTNRMKLYVFSND
                     DRAQALLVAVYDNLGKGASGAAVQNLDLMLGLHS"
     gene            complement(7649..>8507)
                     /locus_tag="VGM46_09485"
     CDS             complement(7649..>8507)
                     /locus_tag="VGM46_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEY4192863.1"
                     /translation="QQIPQYAELNKGMTVDSFKSIFWTEWAHRLLARGVGFIFALPLL
                     FFWATRRIERGLAPKLVGILLLGGLQGAVGWWMVASGLVNRVDVSQYRLATHLTLAAF
                     IFTATMVVARGLAPYSQPAAGRATQRLAGILVVLVLIQLYLGGLVAGLDAGLSYNTWP
                     LMDGKIIPGDLFILDPAWRNLFENPKTVQFIHRLGAYVVFLVALWQMIATLRREPRTT
                     HARRAVVLFLLVLVQASLGIGTLVMHVPMDMALTHQAFALVVLGFAAAHWRGAKGAYP
                     LPDEAGSSR"
BASE COUNT         1669 a         2697 c         2574 g         1567 t
ORIGIN      
        1 tagccctggc ggtcgctctc gtcgcgaatg tcggaaatgc cctcgatgcg cttgtcgcgc
       61 accagttcgg ccatcttctc gatcatcgtc gccttgttca cctgataggg aacctcggtg
      121 atgacgatgg attcgcggtc gttgccgcgc ggctcgacgc tggcgcggcc acgcatgacg
      181 atggagccgc gcccggtggc gtaggcgctg tagatgcccg agcggccaag cacgatggcg
      241 ccggtgggga aatccggccc cggaatgatt tccatcagct ccggcaggtc gatcgcgggg
      301 ttgtcgatca gcgcgatggc gccgttgcag acctcgccca gattatgcgg gggaatgttg
      361 gtggccatgc cgacggcgat gccgccagag ccattgacca acagattggg gaagcgcgcc
      421 ggcaacactt tcggctcgct gccggaagcg tcgtaggtat cctggaaatc gacggtttcc
      481 ttgtcgatgt cctccagcaa ttcatgcgcg accttggtaa ggcgcgattc ggtgtagcgc
      541 atcgccgccg gcggatcgcc gtcgatggag ccgaaattgc cctgcccgtc gaccagcggc
      601 acgcgcagcg accaatcctg cgccatgcgc accaaggcgt cgtagatcga ggcgtcgcca
      661 tgcggatggt atttacccat cacgtcggcg acagggcgcg ccgacttcac atatttgcgg
      721 ttccagtgat agccgctctc atgcgctgca tagaggatgc ggcggtggac cggcttcaga
      781 ccgtcgcgca cgtcaggcag cgcacggctg acgatcacgc tcatggcgta atcgaggtag
      841 gaacgctgca tctcctcgat gatggagatg ggttcgatgc ctgtcggacc gccttcggcg
      901 ccgcgaggtg gtttctggtc agtcaaatcg gaatcacgca gattgaggaa tcactggctt
      961 tttatatagg aagcccagcg atatctccaa tgattcgcag agttttccag aactgaatca
     1021 tgtcggaatt tcaaatggat atgcactgtt ttatcggact gtgacagcat ggccggcaag
     1081 atcgcgcgaa tgcagcgccg ccatctgccg gaaaacggga ctggcactgt tgccgcgatc
     1141 acgctagctt gccgcggggg agaccagccc gtgccaagtt tcgataatat tttcaatgcc
     1201 ttcgtcacca ttttggtgac catcgaccca cccggccttg ccccgctttt cctggcggtg
     1261 acgcgcggca tgaaccgcga cgaacgccgg caggtctcca tccgcgcctc gatcatcggc
     1321 tttgcggtga tggcgctgtt tgcggtagcg ggcgcgtcga tcctgtcgat attcggcatc
     1381 accctgcccg ccttccgcgt cgcgggcggc ttcctgttgt tcttcatcgc cttcgaaatg
     1441 gtgttcgagc ggcggcagga ccggaaggaa aagatttccg atgtcgccat caccaaggac
     1501 atgatccaca atatcgcggc ttttccgctg gcaatcccac tcattgcggg gccaggcgca
     1561 atctcggcga cggtgctgct ttccggctcg ttccagggtt ttgccgcgca ggccaccctt
     1621 gtcgggatca tcctggcctg tcttgtcttg acctacctcg tcttcgtgct gtcggaacgg
     1681 atcgatcgtt tcctcggaca gacgggccgc tcgatcctga cgcggctttt gggcgtcatc
     1741 ctggccgcac ttgccgtgca gttcgtggcc gatggcgtca aggcgctggt ggcgggctag
     1801 gcttatccag cctttctatt gcggcgctcg tagaacatgg cgagcgtttc ggccatcaag
     1861 gcaggctttt cctcgccttc tatctcgatg gtgttggcga ccttcatcag atattgcccc
     1921 ggccccttgt cctccatgga cagaagcgtc accctcagcc gcacccgcga gccgaccttg
     1981 acgggcgaca aaaagcgcac cctgtctagg ccgtaattga aggcggcttg cgtgttttcg
     2041 ggaatgacgc ccatttcctg gctgaggcag ccgatgatcg acagtgtgag atagccgtgc
     2101 gcgatggtgg tgcggaaagg actttgccgc ttcgcgcgca ccggatcggt atgtatccac
     2161 tggcggtcgc cggtacaggc ggcaaactgg tcgatgcggg cctgatcgac ctccatccag
     2221 tcggatacac ccagttcctg gccctccatg gtcctgagct gctcataagt cgggggcgtt
     2281 cgcgtcatgt cccgcaattt tctccgttcg aaaccagctg tataaaaatc aaacaggcag
     2341 acaaggtcaa ttcggcgctg acactttcgg ttcccctcaa gcgcgttctt tgcgcgtggc
     2401 gctatttttt cttctcatat tcctcgcgaa cctcgaccat cgcctccttg atgcggttgc
     2461 gcaatatctc gatcgattcg gtgccggaac tcttcaccag ttcggcgacc tcgcccgcat
     2521 tgctgaccgc gctttcaaag gctttgcggg caaactccgc atgcctggcg atcaattctt
     2581 cagggtttcc cggcgtttta tagtgcttcg ccatttcact cgtctggcgc agcgtatcct
     2641 cgagcatgtc gcgctgcttt cgcatcaacg ccgccgtgcc ggtcgcggtc accctggcgg
     2701 atttctccag cgcgtcgaaa ttcctgcgct gatgctcgat tatggcatcg acgtcgagat
     2761 tcggcagctt caggtcgcgg ccaaacctgg caaacatatc catgaaggag ggttcttcgg
     2821 gcttttttgc catgttcgca tttccgcgtt gagatttacg gcgcttcccg gatgaagata
     2881 gacaagcagc cggtatcgtc aattggccag cagcagttgc accgtgtaga gcgccaagcc
     2941 ggccagcccg ccaattatca tgccgttgaa gcggatatat tgcaggtcgc ggccgatatt
     3001 gatctcgatc agccgggtaa gctgggccag atcccagcgc ttgacctgat cggcgatgaa
     3061 agtggatacg ccgcttttct ggctttcgac aaaggccgac agggcgacga caaaaccctt
     3121 gttcatatcg gcgcgaacgc tggcgtcgcc ggtcagatgc ctgccgatct cgacgaacat
     3181 gccggccaga tgcgccctga ttaccgattc gtccgactgc gcgtcctttt cgatgaattg
     3241 gcgaaggctc gtccacatcg cctgcgccat gtcgcgcagt tcggggcggg cgagaaaatc
     3301 gcgcttcagc ttttcggcgc gcctcgcata gtctttcgag gtcttcagcc gttcgatgaa
     3361 tgccgccacg aagccgtcga actcggcgcg caacggatgg tcaggctctg cccgcacctc
     3421 ctccagcaga gatgccgcgg aagcgacgat cttcttcagc aggtaggcat cggcccgaaa
     3481 caggttgaaa agcgagggca attcctcgcg gattttctcg cgcatgacag ccagcgcctg
     3541 ctcgtcgcta aggaagcggc caatcaccct ggtaaactcg tcgaacagtt tctggtgacg
     3601 ccggtcgtcg gtaaaggccg acagcaattc ggcggcgagc ggcgccagtt gcaccttttc
     3661 ggcctgttcc agcatgcggc tggaaacgaa gccgcgcagg ccggactgct ccacggcgtc
     3721 cagcgtctgc ggcaccagcc gggcgacgaa gcgcgacagg ccggcagcac ggccgggatc
     3781 ggacagccag tcggcgacga gagccgcgaa atccacctcg tccagctttt cgcgcaccgg
     3841 gccgggtgca aggaagttca cctctatgaa gcggccgaga ttttcggcga tgcggttctg
     3901 gttagcaggg atgatggcgg tgtgcgggat cggcaggcca agcggccgcc gaaacagcgc
     3961 gaccaccgcg taccaatccg ccagcccgcc gatgacggcc gcttcggcgc aggcggcaac
     4021 gaagcccagc cccggataga ggccctgaaa cgaatgagcg gcgatgaaca ctaccacgca
     4081 cagcaccagt gcagcacttg ccatgaactt ggtgcggcgc agcgccgaaa gcttggccga
     4141 ctgctcgctg tcggggtcga tggggctgaa gcccgcctgt gtcatcgttt accgttccgg
     4201 ctttcgattg cgcgaactgc caatcaaatg cgctcggcga gccgccgaaa caatgccagc
     4261 gccggcattt cgacaaaacg ccatgtgaac cacgacgcga caagcgtagc ggcgaccatc
     4321 agcgcgatgg ccgccagccc tgtccacggc gcatcggggc cgatgccgat cgccgtgtcg
     4381 ccgcgcagcg acaaggggcc gaccaggccg aggccgaact tgcggtccac caggccggcg
     4441 acgttgatca tgcggccctg cacgaaaatg tgaaccatgt agatcgaata ggatagtgcg
     4501 ccgagcgtca gcatcgggcg ggtgcgcaac aggctgctga cccagccgcc ttcatgggcg
     4561 aagacataaa gcgccaccgc aaacagcaac ggcgcggcga tgccgccttt attgtcgctg
     4621 aaatcgacca cgaaaagcgc gatgacggca atcatcgcca attcaagcag ggtccagatc
     4681 aaccggtcgg ttccctgccc cgcgtccctt cgcgcctcca ccagcctgtc gtgctgaaac
     4741 catgacagca gcgcgccgat ggaaaagccg tagaggcaac gcaccaggcc gaaatcatag
     4801 gagacgtcca tggtgtcggc aaaacgcaac agcacgagcg gaccgacgac agcagcgcat
     4861 gccagcaccg cccatgcgcg tttgcgggca acgaaaacga ccgccgcaaa gaaaaggtag
     4921 gcgaagaatt ctgtcgagat gctccagctt gggccattcc acgtcagcct gtcttcaagc
     4981 ccggtgcctt gcagcagaag gaggttggcc accaggctgc cgatactgtt gtcttcggtg
     5041 aaaggcgcgg cgccagcgcc gcgcaattgc ggcagcatca ggcgaagcag ttcgaaggcg
     5101 acaaacgcca gaagcatgaa caggtgcaac ggatagagcc ggccaaagcg aaccagcgcg
     5161 aaacgcgcca ccttcttcgg ctcgtccagc ctgccgccat agctgctggc gatgacgaag
     5221 cccgacagca cgaagaagaa atcgacgaac agataggccc cgccgacaaa ggcgctttgc
     5281 gagatggcgg aactggttgg aaagtgcagc agcgccacca gcaacgcgca gacgccccgc
     5341 cacgaatcca gcaccaggaa gcgcacctgc gaggcggaag ccgtctcgcc tctcgatccg
     5401 gcataggccg gctgcgtcat cgccactgac tgcgtcctca tccacatctc gcttggcacc
     5461 gatatttcat cgtgcgcttt ccgatagttg tcatgcgcct gtgcaaagcc acccgctatg
     5521 ctggcagact tgccggccaa ctgggcgaac cttggcgctt ttcctcgcct gtctttgcct
     5581 ttatacgcgc cattccgata aaattcgtga atcgccgccc gaagaaccga ggaaacgtga
     5641 tgccgaacag cactatcgac cacgccttca acgcccgcgc catgaccggg gcctcgcttg
     5701 agccgaccta tgccggggcg ctttccttca tgcggcgaaa atataccaag aacgtgaagg
     5761 gcgcggatgc ggtggtgtgg ggcattccct tcgacgccgc cgttaccaac cgtccgggcg
     5821 cgcgcttcgg gccgcaggcc attcgccgcg ccgccgccat cctcgacaac gacccgcaat
     5881 atccgttcgc gcgcgatatt ttcgagcacc tggcagtcgt cgattatggc gactgcctgc
     5941 tcgacagcgg caaccaccag aaaacacccg ccaccatcga gcgcgaagcc gccaaactgc
     6001 tgaaatcggg cgcgttcctg ctgacgctgg gcggcgacca tttcgttacc tggccggtgc
     6061 tcaaggcgca tgccgaaatc cacggaccgc tggcgctggt acagttcgac gcgcaccagg
     6121 acacctggcc cgacgacggc aagcgcatcg accacggctc tttcgttggc cgcgccgtca
     6181 aggaaggcat catcgatccg gaccgttcga tccagatcgg catccgcacc catgcgcccg
     6241 atacgttcgg catcaagatt ctctacggcc atgaaatcga ggagatgcgc gcttccgaca
     6301 tcgcctatgc catcgtggag cgcaccggcg gcaagaaaac ctatgtgaca ttcgacatcg
     6361 actgtctcga ccccgccttt gcgccgggaa ccggcacgcc ggtggccggt gggccatcgt
     6421 cggccaaaat cctgtcgacg ctgcgccagc ttgggcagat cgatattgtc ggcgccgaca
     6481 ttgtggaggt tgcgccggcc tatgatcatg ccgatattac ggctatcgcc ggctcgacca
     6541 tcgctatgca gtatctcgga cttctggcgg agcggaaggc gcggctctcc gagaccgaga
     6601 acagtgctgc tgcaatgaat ccgggaagcg gcatataacc ttgaaattca tggtatttct
     6661 ggcggacatc aggcaatgaa accgaaaatc ttcatcgacg gcgaacacgg caccaccggc
     6721 ctgcaaatcc gcgcgctgct ggccgaccgc ggcgacctcg aaatcctgtc cataccgacc
     6781 gggcggcgca aggaaacggc ggcgcgcgcc gaatacctca atgccgccga tgtcgccatc
     6841 ctgtgcctgc ccgacgatgc ggcgcgcgaa agcgtctcgc tcatcgccaa cgacaagacc
     6901 aaggtgatcg acgcctccac ggcgcaccgc gttgccgaag gctgggaata tggctttgcc
     6961 gaactggaca aggaccagcc gcagcgcatc gcctcggcaa agcgcgtcgc caaccccggt
     7021 tgctggccgc aggggccgat cgcctcgctg cgcccgctga tcgatgcggg gctgctgccg
     7081 gcggactatc cggtcaccgt caacggcgtc accggctatt ccggcggtgg ccgcccgatg
     7141 atcgaggact atgtcgccaa gggcgaggac gcgccggagt tcctgcccta cggcctgacc
     7201 ctgcaccaca agcatgtgcc cgaactcaag acttattcaa ggcttttgcg cgatccgatc
     7261 atgcagccgg cagtcggcaa tttcgcgcaa gggatgctga cggtggtgcc gctgcaactc
     7321 ggcaatctcg actatgtgcc aagcggcgcc gagctgcacg cggcgattgg cgaccacttc
     7381 gccggcattg ccggcagcgt ggttgaggtc gcgccgttcg cgcaagtgga gcggatggcc
     7441 gaaatcgacc ccgaacacct aaacggcacc aaccgcatga aactttacgt cttttccaac
     7501 gacgacaggg cgcaggctct gctcgtcgcc gtctacgaca atctgggcaa gggcgcgtcg
     7561 ggcgctgccg tgcagaacct ggacctgatg ctcgggcttc attcctgacg ccgaccggcg
     7621 atggcgcgaa acggtcgcgc catgcggttc agcggctgga accggcctcg tcgggcagcg
     7681 gataggcgcc cttggcgccg cgccaatggg cggcggcgaa gcccagcaca accagcgcaa
     7741 aggcctgatg cgtcagcgcc atatccatcg gcacatgcat gaccagcgtg ccgatgccga
     7801 gcgaggcctg caccagcacc agcaagaaca gcacgacggc gcggcgcgcg tgggtggtgc
     7861 gcggctcgcg gcgcagcgtg gcgatcatct gccacagcgc gacgaggaag acgacgtaag
     7921 cgccgagacg gtggatgaac tgcacggtct tgggattttc gaacaggttg cgccaggctg
     7981 gatcgagaat aaacaggtcg cctggaatga tctttccgtc catcagcggc caggtgttgt
     8041 agcttaagcc ggcgtcaagc ccggcaacca gcccgccgag ataaagctgg atcaacacca
     8101 gcaccaccag aatgccggcc agccgttgcg tcgcgcggcc ggcggccggt tgcgaatagg
     8161 gcgcaagccc acgcgccacg accatcgttg cggtgaagat gaaggctgcc agggtcaggt
     8221 gggtggccag ccggtactgg ctgacatcga cacgattcac cagtccggag gccaccatcc
     8281 accagccgac cgcgccttgt agcccgccca gaagcaatat gccgacgagt ttcggcgcaa
     8341 ggccgcgttc gatgcggcgc gttgcccaga agaaaagcag cggcagcgca aagatgaagc
     8401 cgacgccccg cgccagcagc cggtgcgccc attccgtcca gaagatcgac ttgaaggaat
     8461 cgaccgtcat gcctttattc aactcggcat attgcgggat ctgctgg
//
LOCUS       DASYCS010000302         2093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4862243,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000302 DASYCS010000000
VERSION     DASYCS010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4862243"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VGM46_09490"
     CDS             complement(<1..387)
                     /locus_tag="VGM46_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY4192864.1"
                     /translation="MNLFRKKLPEADDAPRLRRSLSAWDLTFMGIGQMIGAGIFVLTG
                     VAAATQAGPAIILSFLAAGIACGFVAFSYAELAACVGGCGGAYGYAYALFGEFVAWIV
                     GWNMTLGLGVAVAAVANGWSGYFNNAL"
     gene            complement(428..1027)
                     /gene="hisB"
                     /locus_tag="VGM46_09495"
     CDS             complement(428..1027)
                     /gene="hisB"
                     /locus_tag="VGM46_09495"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEY4192865.1"
                     /translation="MRTATIERKTRETEILASVDLDGTGVYDIDTGIGFLDHMLESFS
                     RHSAIDLKVRAQGDLHVDFHHTTEDTAIVIGQAIKRAAGDFAGIRRFGWVLMPMDETL
                     TRVAIDISNRPYLIWKVDFPRPKIGEMDTELFREWFQALAQNAGICLHVENLYGENSH
                     HIVETCFKGTARALRQALEIDERLDGAVPSTKDTLSDDK"
     gene            1165..1710
                     /gene="hslV"
                     /locus_tag="VGM46_09500"
     CDS             1165..1710
                     /gene="hslV"
                     /locus_tag="VGM46_09500"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEY4192866.1"
                     /translation="MGEALWHGTTILSVRKGGQVVIAGDGQVSMGNTIVKANAKKVRR
                     LGKGDVIAGFAGATADAFALFERLEAKLESHPGNLARACVELAKDWRTDRYLRRLEAM
                     MAVADRNSSLVLSGTGDVLEPEDSLIGIGSGGPYALAAARALLPLDLPAEEIARRAMK
                     IAAGICVFTNDNVTIESLATG"
     gene            1728..>2093
                     /locus_tag="VGM46_09505"
     CDS             1728..>2093
                     /locus_tag="VGM46_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY4192867.1"
                     /translation="MSSFFTPREIVSELDRYIVGQHDAKRAVAIALRNRWRRQQLPES
                     LREEVLPKNILMIGPTGVGKTEISRRLAKLAQAPFLKVEATKFTEVGYVGRDVEQIVR
                     DLLEVGIGLVRSAKRKDVEA"
BASE COUNT          405 a          635 c          671 g          382 t
ORIGIN      
        1 cagcgcgttg ttgaaataac cggaccagcc atttgccacc gcggcaaccg ccacgccgag
       61 ccccagcgtc atattccagc cgacgatcca ggcgacgaat tcgccgaaca gggcataggc
      121 ataaccatag gcgccgccgc atccccccac gcaggccgca agctccgcat aggaaaatgc
      181 gacaaagccg caggcgatgc ccgcggccag gaacgatagg atgatcgccg gtcccgcctg
      241 cgtcgcggcg gcgacgccgg tgagcacgaa gattcccgcg ccgatcattt ggccgatacc
      301 catgaaggtc aggtcccacg cgctcagcga gcggcgcagt ctcggcgcat cgtcggcttc
      361 cggcaatttc ttgcgaaaca gattcatgaa gcggacttag gcacaggcca cctgtgaatc
      421 gccaaaccta cttgtcgtcc gacaacgtat ccttggtgga gggcacggca ccgtcgagcc
      481 gttcgtcgat ctccagcgcc tggcgcagcg cgcgcgccgt ccccttgaag caggtctcga
      541 cgatgtgatg gctgttctcg ccatagagat tctcgacgtg caggcagatg ccggcattct
      601 gggcgagcgc ctggaaccat tcgcggaaca attcggtgtc catctcgccg atcttcggac
      661 gcgggaaatc gactttccag atcagatagg gccggttcga aatatcgatg gcgacgcgcg
      721 tcagcgtctc gtccatcggc atgagcaccc agccgaagcg gcgtatccct gcgaaatcgc
      781 cggccgcgcg tttgatggcc tggccgatca cgatggcggt gtcttcggtg gtgtgatgga
      841 aatcgacgtg cagatcgccc tgcgcgcgga ccttcaggtc gatcgccgaa tgccgcgaaa
      901 agctttcgag catatggtcg agaaaaccga tgccggtatc gatgtcgtaa acgcccgtgc
      961 cgtcgagatc gacggaggcg aggatttcgg tctcgcgggt cttgcgttcg atggtggcgg
     1021 tgcgcatgag caaagcgttc tttcaggaag cctgcgagct atatcgcatg aaatccagcg
     1081 cgtcgaaggg tttaagtggg accggttgcg caacgcccag cggcggccca tatccttgac
     1141 ctgccggttc tagggagttg ttcgatgggt gaggcgctgt ggcatggcac gaccattctt
     1201 tccgtccgca aaggcgggca ggttgtgatt gccggcgatg gccaggtgtc gatgggcaac
     1261 accatcgtca aggccaatgc caagaaggtg cgccgtctcg gcaaaggcga tgtgatcgcc
     1321 ggttttgccg gggcgacggc ggacgcattc gcattgttcg aacgcctgga ggcgaaactg
     1381 gagtcccatc ccggcaacct cgcgcgcgcc tgcgtggaac tggccaagga ctggcgcacc
     1441 gaccgctatc tgcgccggct cgaagccatg atggcggtgg cggatagaaa ttcgtcgctg
     1501 gtgctgtcgg gcacgggcga tgtgctggag cccgaagaca gcctcatcgg catcggctcg
     1561 ggtggtccct atgcgctggc cgcggcgcgc gcgcttctgc cgctggatct gccggcggag
     1621 gaaatcgccc ggcgcgcgat gaagatcgcc gccggcattt gcgtcttcac caatgacaac
     1681 gtcaccatcg agagcctggc cacaggctga gaccaaggaa tcgtttgatg agttcgtttt
     1741 ttaccccccg tgaaatcgtt tccgagctcg accgctatat tgtcggccag cacgatgcca
     1801 agcgcgcggt cgcgattgcg ctgcgcaacc gctggcgccg ccagcaattg ccggaaagtt
     1861 tgcgcgaaga agtgctgccc aagaacatcc tgatgattgg gccaaccggc gtcggcaaga
     1921 ccgagatctc gcggcgcctc gccaagctgg cccaggcgcc gttcctgaaa gtggaagcca
     1981 ccaaattcac cgaagtgggt tatgtcggcc gcgatgtcga acagatcgtg cgcgaccttc
     2041 tggaagtggg catcggtctg gtgcgttcgg ccaagcgcaa ggacgtcgag gcc
//
LOCUS       DASYCS010000303         6651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1388926,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000303 DASYCS010000000
VERSION     DASYCS010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 6651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6651
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1388926"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VGM46_09510"
     CDS             complement(<1..396)
                     /locus_tag="VGM46_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEY4192868.1"
                     /translation="MTSPSGSKPAEAAAFLAAHPEIEAFDIVLIDANGVGRGKIIRRH
                     ELPSLFEAGRHMPISVLGLDIAGEDVHETGLIWDCGDGDLRAWPIPGTLVPLHGTQPP
                     RGQVMMSMYHLDGAPMTSDPRHALARQVEI"
     gene            complement(481..1455)
                     /locus_tag="VGM46_09515"
     CDS             complement(481..1455)
                     /locus_tag="VGM46_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEY4192869.1"
                     /translation="MPTVAPLDLDGHCIAAVFLADVPHFAMADGSVHRLDHGQKTSQA
                     NDGLLAAFHDAANDRLLTSGEDGKVFAVKAEGQVEELASAGRKWITSVSAGPQGAVAY
                     AAGKTAFMRFSDGKVKEFQHPRSVEGIAFSPKGMRIGVARYNGATLHFPAAEGKPVEL
                     EWAGAHTGIIFSPDGNFVVTTMQENALHGWKLVDAKHMRMSGYPGKVKSLSWSAKGKW
                     LASSGAPAAIVWPFSGKDGPMGKAPLELGTRGNAMVTCVACHPSDDVVAIGYDDGMVI
                     AARFADAKEVLLRRPGKGAITAMMWDKAERRIAFGSAAGDCGIIDISA"
     gene            complement(1463..2542)
                     /locus_tag="VGM46_09520"
     CDS             complement(1463..2542)
                     /locus_tag="VGM46_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006325367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY4192870.1"
                     /translation="MSEAQTQIPVTVLTGYLGSGKTTLLNRILSENHGKRYAVIVNEF
                     GEIGIDNELILNTDEELFEMNNGCVCCTVRGDLIRVVEGLMRRPGRFDAILVETTGLA
                     DPVPVAQTFFMDDDVRSKTRLDAVVALVDAKHLPLRLKDSKEAEDQIAFADVIVLNKT
                     DLVTPEELRAVEATIRAINPAARIHRTTRAGLPLSDVLDRGAFDLSRALENDPHFLEA
                     HDEHGHDHECGPDCDHDHHHHGHDHHGHDHHHDHAQPSAIHDVTVQSVSLRGGEMDPQ
                     KFFPWIEKITQMEGPNILRLKGIIALKGDDERYVIQGVHMIIEGDHQRAWKDGEKHES
                     RLVFIGRELDAERLKKSFDACQAAA"
     gene            2710..3888
                     /locus_tag="VGM46_09525"
     CDS             2710..3888
                     /locus_tag="VGM46_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY4192871.1"
                     /translation="MEFFDVVILGAGAAGMMCAIEAAKRGRTVLVMDHASAAGEKIRI
                     SGGGRCNFTNIHAGPKNFLSQNPHFCISALKRYTQADFVALVKSHGIAFHEKTLGQLF
                     CDGSATQIIAMLLAEMNRHGAMLRLGTRVENIEKAEAGFVISLENGPVRCASLVVACG
                     GKSIPKMGATGLGYEIAAQFGLRVVETRPALVPLTFEPNTLERLRPLAGIAVDAVISC
                     GKTRFSEAMLFTHRGISGPSILQISSFWREGDEIAIAMLPGIDVFEALRAARAANGKQ
                     AVQTVLAAFMPRKLAQTIAEQLGPQRNLADMPDRELRRASAAVNDWRIKPSGSEGYRT
                     AEVTLGGVDTRDLDSRTMAAKSVEGLYFIGEVVDVTGWLGGYNFQWAWSSGWCAGQVA
                     "
     gene            4066..4536
                     /locus_tag="VGM46_09530"
     CDS             4066..4536
                     /locus_tag="VGM46_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015314298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY4192872.1"
                     /translation="MAKPDKTATMSRLHSAARLARTALAARLLAHGFYAGQDQIMLAL
                     EQEDGQTPGTLAGRLGVRPPTITKTINRLQAQGFLEKRASESDARQAHIFLTELGRGT
                     IRAIEKSVKKTEKQALKGLDKKEQKSLFKMLARIEANLSDGEIDLSDDIPDIDD"
     gene            4731..5615
                     /locus_tag="VGM46_09535"
     CDS             4731..5615
                     /locus_tag="VGM46_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY4192873.1"
                     /translation="MIATYIGFTFLDSSSKFLVLAGMAPLFIAWCRFTGHVVLVGVLF
                     RGWKNSRRFRAKNLPAHLLRGAFLGGSTVFNVFALRYLQLAETTSIYFFGPMVITALA
                     GPMLGEWAGWRRWLAIWIGFAGVLVITRPGVGVFGIGHVFALCSMLSNCFYVIMTRRM
                     SATETPESLILFAGLSPVILLLPTLPFSVTLPATPVQWAILLMLGVFGGVSHWFVVKA
                     YSIATATALAPYPYSQMIWMIAFGWLIFHQFPDMWTLAGAAIIVASGLYIVHREHRLR
                     VRNRAAPDAETEVLAKTL"
     gene            5741..>6651
                     /locus_tag="VGM46_09540"
     CDS             5741..>6651
                     /locus_tag="VGM46_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY4192874.1"
                     /translation="MSTATVSLALRDSPLVAASTRDRIKEHARAIGYIYNRRAASLRT
                     SRSGIVGVIVHDIMNPFYAEILRSIESELDRSRQTFILSNHYDQLEKQRIFIDTLLQL
                     GADGVIMSPAIGTPASDILTAEENGLPAVLIARDVEEAHVPVFRGDDAYGTALATNHL
                     ISLGHKRIVMIGGTEQTSTGHDRYQGYVNAMEAAGLEVKPSWRISGPRTKQAGFEAAG
                     QFLALKDKPTAACCWNDLVAIGLMNGIARAGLVPGVDISVTGYDDLEEASIATPALTT
                     VWNGQREVGRRAASALLDKLNGESVRTP"
BASE COUNT         1159 a         2104 c         2088 g         1300 t
ORIGIN      
        1 gatttccacc tggcgggcca gcgcgtggcg cgggtcggag gtcatcgggg cgccgtcgag
       61 atgatacatc gacatcatca cctgcccgcg cggcggctgg gtgccgtgca gcggaaccag
      121 cgttccgggg atcggccagg cgcgtaggtc gccgtcgccg cagtcccaga tcagcccggt
      181 ttcgtggacg tcctcgccgg cgatgtcgag gccgagaacc gagatcggca tatgccggcc
      241 ggcctcgaac agcgacggca gttcgtgtcg gcggatgatc ttgccgcgcc caacgccgtt
      301 ggcgtcgatc agcacgatat cgaaggcttc gatctcggga tgagcggcca ggaaagctgc
      361 tgcttcggcg ggcttcgaac ccgaaggtga ggtcatgatg gcgctcggtt gaatgccccg
      421 tcttgtctca tgccattcac attgccgcaa ccaccggcgg tttggcggga aacaaaccgt
      481 tcaggcggaa atatcgatga tgccgcaatc gccggccgcg ctgccgaagg cgatgcgccg
      541 ctcggccttg tcccacatca tggcggtgat ggcgcccttg ccggggcggc gcagcagcac
      601 ttccttggcg tcggcaaagc gggcggcaat caccatgccg tcatcatagc cgatggcgac
      661 gacgtcgtcg ctcgggtggc aggcaacgca tgtcaccatc gcattgccgc gcgtgccgag
      721 ttccagcggc gctttgccca tcgggccgtc cttgcccgaa aacggccaga cgatcgccgc
      781 cggcgcgccg gagcttgcca gccatttgcc ctttgcgctc cacgacaggc tcttcacctt
      841 gcccggatag ccggacatgc gcatgtgctt ggcgtcgaca agcttccagc catgcagggc
      901 gttttcctgc atggtagtga cgacgaaatt gccgtccggc gaaaaaatga taccggtatg
      961 cgcgccggcc cattccagtt ccaccggctt gccttcggcc gccgggaaat gcagcgtcgc
     1021 gccgttgtag cgggcgacgc cgatgcgcat gcctttcggc gaaaaggcga tgccctcgac
     1081 cgaacgcgga tgctggaatt ccttcacctt gccgtcgctg aatctcatga aggcggtctt
     1141 gccggcggca taggcgaccg cgccttgcgg tccggccgac acgctggtga tccacttcct
     1201 gccggcgctg gccagttcct cgacctggcc ttcggccttt acggcgaaca ccttgccgtc
     1261 ttccccgctg gtgagcaggc ggtcgtttgc cgcgtcgtgg aaagcggcca gcaggccgtc
     1321 attggcctgg ctggtcttct ggccgtggtc gagccggtgg acggagccat cggccatggc
     1381 gaaatgcggc acatcggcga ggaacaccgc ggcgatgcaa tgaccatcga ggtcgagcgg
     1441 ggctactgtc ggcatgtttg tgtcaggccg ccgcctggca ggcgtcgaag cttttcttca
     1501 gccgttcggc gtcgagttcg cgcccgatga acaccagccg gctttcgtgc ttttcaccat
     1561 ccttccaggc gcgttgatgg tcgccctcga tgatcatgtg gacgccctgg atgacatagc
     1621 gctcatcgtc gcccttgagc gcgatgatgc ctttcagccg caggatgttg ggaccttcca
     1681 tctgagtgat cttttcgatc caagggaaga atttctgcgg gtccatctcg ccgccgcgca
     1741 gcgaaaccga ctgcaccgtc acgtcatgga tggccgaagg ctgggcgtgg tcgtggtgat
     1801 ggtcgtgccc gtggtggtca tggccatgat gatggtggtc gtggtcgcaa tccgggccgc
     1861 attcgtggtc atgcccatgc tcgtcatgcg cttcgaggaa atgcggatcg ttttccagcg
     1921 cgcgggaaag gtcgaacgcg ccgcggtcga gcacgtcgct cagcggcagg ccggcgcggg
     1981 tggtgcgatg gatcctggcc gccggattga tggcgcggat cgttgcttcc acggcgcgca
     2041 gttcttcggg tgtcaccagg tcggtcttgt tgagcacaat gacgtccgcg aaggcgatct
     2101 ggtcctcggc ttccttcgag tccttgaggc gcaacggcag gtgcttggcg tccaccagcg
     2161 ccacgaccgc gtcgagcctg gtcttggaac gcacgtcgtc gtccatgaag aaggtctgcg
     2221 ccaccggcac cgggtcggcg aggccggtcg tctcgacgag gatggcgtcg aagcggccgg
     2281 gccggcgcat caggccttcg acgacgcgga tcaggtcgcc gcgcaccgtg cagcagacgc
     2341 agccattgtt catctcgaac aattcctcgt cggtgttcag gatgagttcg ttgtcgatgc
     2401 cgatctcgcc gaactcgttg acgatgacgg cataacgctt gccgtggttt tccgaaagaa
     2461 tgcggttgag cagggtggtc ttgccggaac cgagatagcc ggtaagcacg gttacgggaa
     2521 tttgcgtctg ggcttcgctc atgtcggacc tcgaagggat ttgtcgcctc catataagat
     2581 ggtggtcggc gatttgcacg attaatcagg ctcgtttcat attgggccag atcacgaccc
     2641 gttatgctga aaattgcggt gcaacgggca ggtctcacgc tatagagagc ctgtatgtga
     2701 gggccggctg tggaattctt cgatgtcgta attctgggcg cgggcgcggc cggcatgatg
     2761 tgcgccatcg aagcggcaaa gcgcggccgc accgtgcttg tcatggatca tgcgagcgct
     2821 gccggtgaaa aaatccgcat ctcgggtggt ggccgctgca acttcaccaa tatccacgcc
     2881 ggcccgaaga acttcctgtc gcagaacccg catttctgca tctcggcgct gaaacgctat
     2941 acgcaggcgg atttcgtggc gctggtcaaa agccatggca tcgccttcca cgaaaagacg
     3001 ctcgggcaac tattctgcga cggttccgcc acgcagatca tcgccatgtt gctggcggaa
     3061 atgaaccgcc atggcgcgat gctgaggctc ggcacgcgcg tggaaaatat cgaaaaggcc
     3121 gaagccggtt tcgtcatttc cctggaaaac gggccggttc ggtgcgcttc gctggtggtg
     3181 gcctgcggcg gaaaatccat tcccaagatg ggcgcgaccg gcctcggcta cgagatcgcc
     3241 gcgcagttcg gcctgcgcgt ggtcgagacg cggccggccc tggtgccgtt gaccttcgag
     3301 ccgaacacgc tggagcggct gcggccgctg gccggcattg cggtggacgc cgtcatttcc
     3361 tgcggcaaga cgcgcttttc ggaagccatg ctgttcaccc atcgcggcat tagcggcccg
     3421 tccatcctgc aaatctcgtc tttctggcgc gagggcgacg aaatcgccat cgccatgctg
     3481 cccggcatcg atgttttcga ggcgttgcgt gccgcgcgcg ctgcaaacgg caagcaggcc
     3541 gtgcagacgg tgctggcggc gttcatgcca aggaaacttg cccagactat tgccgagcag
     3601 cttggcccac agagaaatct ggccgatatg cccgacaggg agcttcgccg cgcttcagcc
     3661 gccgtcaacg actggcgcat caagccttcc ggctccgaag gctaccggac ggctgaagtg
     3721 acgctcggcg gcgtcgatac gcgcgacctc gattcgcgca ccatggccgc gaaatcggtc
     3781 gaaggtctct acttcatcgg cgaagtggtc gatgttaccg gctggctcgg cggctacaat
     3841 ttccagtggg cctggtcgtc cggctggtgc gccgggcagg ttgcgtaatc ggccacggcc
     3901 aggaaagtca gccggataac gagtgtcatc taactgtaat atgcatctgg cataagcatg
     3961 ccgctacggt tcgcttgccg gcaaaaggct ttttgcgggc aaaaaccggg cgattgcaac
     4021 gatggcgcat gatatgctgc cgcctgtccg gatcgagggg aagacatggc caagcccgac
     4081 aagactgcga cgatgagccg gctgcattcc gcggcaagac tggcgcgcac cgcccttgcg
     4141 gccaggttgc tggcgcacgg tttttatgcc gggcaggacc agatcatgct ggcgctggag
     4201 caggaagacg gccagacgcc cggcacgctg gccggccgcc tcggcgttcg ccctccaacc
     4261 atcaccaaga cgatcaatcg tttgcaggcg cagggcttcc tggaaaagcg cgcatccgaa
     4321 tccgatgcgc gccaggcgca tatctttctg accgaattgg gccgcggcac catccgggcc
     4381 atcgaaaagt cggtcaagaa aaccgagaag caggcgctga agggcctcga taaaaaggaa
     4441 cagaaatcgc tgttcaagat gctggcccgt atcgaggcga acctgtccga cggcgagatc
     4501 gatctttccg acgacatccc cgacatcgac gattaagcgc cgtctcccgc aagccgcggc
     4561 cacgccgggc ctgacctgat aggccacggc gcttttgacc ttgccggctg ccggcaattc
     4621 cagcaaaaag gcgtcagtcc gcaccgaacg gtcctgcctt gccccacgca gcctcaaagc
     4681 cacaacaggc aaagccagag caggcaacgc cgccggcggc catcgcctat atgatcgcca
     4741 cctatatcgg cttcactttt ctggactcct ccagcaagtt cctcgtgctg gccggcatgg
     4801 ccccgctttt catcgcctgg tgccgcttta ccggccatgt cgttctcgtc ggcgtcctgt
     4861 ttcgcggttg gaagaacagc aggcggtttc gcgccaaaaa cctgcccgcc cacctgctgc
     4921 gcggtgcgtt tctgggcggt tccactgtgt ttaacgtgtt cgcgctgcgc tatctgcaac
     4981 tggctgagac gacctcgatc tatttcttcg ggccgatggt gattaccgca cttgccggcc
     5041 ccatgcttgg cgagtgggca ggctggcggc gctggctggc catctggatc ggttttgccg
     5101 gcgttctcgt catcacccgc ccgggcgtcg gcgtgttcgg catcggccat gtattcgccc
     5161 tgtgctcgat gctgtcgaac tgcttctacg tcatcatgac ccggcgcatg tcggccaccg
     5221 aaacgccgga aagcctgatc ctctttgccg gcctgtcgcc ggtcatcctt ttgttgccga
     5281 cgctgccttt ctcggtgacc ttgcctgcca cgccggtgca atgggcgatc ctgctcatgc
     5341 tcggggtgtt tggcggcgtc agccactggt tcgtggtcaa ggcatacagt atcgccaccg
     5401 ccacggcgct cgcgccctat ccctattcgc aaatgatctg gatgattgcc tttggttggc
     5461 tcatcttcca tcaattcccc gacatgtgga cgcttgccgg cgccgccatc atcgtcgcca
     5521 gcggcctcta catcgtccac cgcgagcacc ggctgcgcgt gcgcaatcgc gcggcccccg
     5581 acgcggagac ggaagtcctg gcaaaaacgc tttgatttgc cgaaaatcga tggcataagc
     5641 cgcgtttaaa ctgtttaaac aacacgaact gtttaaatcg caggggaccg ccgctctggc
     5701 acagaagatc aagctttcga cgatcgccga tgcgctcggc gtgtcgaccg ccacggtttc
     5761 gttggcgttg cgggacagcc cgctggtcgc cgcctcgacg cgcgaccgca tcaaggaaca
     5821 cgcacgcgcc atcggctaca tctacaaccg cagggcagcg agcctgcgca cgtcgcgctc
     5881 cggcattgtc ggcgtcatcg tccacgatat catgaaccca ttctatgccg agatcctgcg
     5941 ctccatcgaa agcgagcttg accgcagccg ccagacgttc atcctttcca accattacga
     6001 ccagctcgaa aagcagcgca tcttcatcga taccttgctc cagcttggcg cggatggcgt
     6061 catcatgtcg ccggccatcg gtacgccggc ttccgatatc ctgaccgccg aggaaaacgg
     6121 cctgccggcg gtgctgattg cgcgcgatgt cgaggaggcc catgtgccgg tgtttcgcgg
     6181 cgacgatgcc tatggcacgg ctcttgccac caaccatctg atttcgcttg gccacaagcg
     6241 catcgtcatg atcggcggca ccgaacagac ctcgaccggc cacgaccgct atcaaggcta
     6301 cgtcaacgcc atggaagcgg cggggcttga ggtcaagccg tcctggcgta tttccggccc
     6361 ccgcaccaag caggccggct tcgaggcggc cgggcagttc ctggcgctga aggacaagcc
     6421 caccgccgcc tgctgttgga acgaccttgt cgccatcggg ctgatgaacg gcatcgcccg
     6481 cgcgggcctg gtgccgggcg tggatatttc cgtcaccggc tatgacgatc tggaagaggc
     6541 gtccatcgcg acgcctgccc tgaccacggt gtggaacggc cagcgcgagg tcgggcggcg
     6601 cgccgccagc gcgctgctcg acaagctcaa tggagagagc gtgcgcaccc c
//
LOCUS       DASYCS010000304         7144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1531602,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000304 DASYCS010000000
VERSION     DASYCS010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 7144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7144
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1531602"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="VGM46_09545"
     CDS             complement(<1..717)
                     /locus_tag="VGM46_09545"
                     /inference="COORDINATES: protein motif:HMM:NF022515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2865 domain-containing protein"
                     /protein_id="HEY4192875.1"
                     /translation="MTERQMKGGAGKLAYRALLLGVIALSCAATSQAMAASRACRQLE
                     AELAGGGGRENPAQVRKYDAAIARQNDEMAKARSRARGAGCGFSLFGGHVAACAALNA
                     SIERMNHNLDTLRSRRTRLAGGGGRRDRARILASLDARGCRDKQPITIRTLEKPAATQ
                     HMLDGDAGNDDRQAGVQDMENISNGKVDIEPLAGLTLENPPHPQGEFRTMCVRTCDGY
                     FFPMSNAASLRDFERDQKNCE"
     gene            complement(814..3159)
                     /locus_tag="VGM46_09550"
     CDS             complement(814..3159)
                     /locus_tag="VGM46_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEY4192876.1"
                     /translation="MEGESTNAKWSDLDEAALHFHKHPVPGKLEIQATKPLGNQRDLA
                     LAYSPGVAAPCLAIRDDPATAADYTGRANLVGVVSNGTAVLGLGNIGPLASKPVMEGK
                     AVLFKKFAGIDVFDIEIDAPEIDRMVETIAALEPTFGGINLEDIKAPECFEVEERLKE
                     RMGIPVFHDDQHGTAIIVAAAILNGLEFAGKSIQDIKIVTSGAGAAALACLNLLVLLG
                     VRIENIWVTDRFGVAYKGRTEEMDRWKDPYVKDTDARTLADVLPDADVFLGLSAAGVL
                     KPEMLKLMAEKPLILALANPNPEIMPDLARAARPDAMICTGRSDFPNQVNNVLCFPYI
                     FRGALDCGASAINEDMKMAAVRAIAALAREEPSDVAARAYSGETPVFGPDFLIPSPFD
                     PRLILRIAPAVARAACETGVATRPITDFPAYIDKLNRFVFRSGLVMKPVFSTAKASAT
                     KRVIYADGEDERVLRAAQVVIEEGIAQPILIGRPHVVDVRLERHGLRIRPGVDFELIN
                     PEDDPRYRNYVDLFIELGGRRGITQEAARTMVRTHTTVIASLALKRGDADAMLCGLEG
                     RFARHLRNVDLIIGPRMGVKDYEFSALSMLISQRGVLFLTDTHVCVDPTAEQIAEMTI
                     LAAEEIRRFGIEPRAALLSLSDFGSRDTPSALKMRKAADILARLAPELQCDGEMHADT
                     ALSEQLRHRVYPHSRLKGEANLLVFPSLDAANITLTALQTMLDALHVGPILLGTDKPA
                     HILTPSVTSRGVVNMTALAVVEATQKAQAVAAEPTRISRQT"
     gene            complement(3322..4101)
                     /locus_tag="VGM46_09555"
     CDS             complement(3322..4101)
                     /locus_tag="VGM46_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY4192877.1"
                     /translation="MDLGIRGKKAIVCASSKGLGRGCAAALAEAGCDLVINGRNAALL
                     AKTAAELREKFGVEVIEIVGDVSKPDVQKALLAACPAPDILVNNNGGPPFRDFRELDR
                     EKILEGLTNNMVTPIELVKVVIDGMAARGFGRIINITSLSVYVPIPGLDLSSGARAGL
                     TSFLAGVARTVMDRNVTINSMLPGKLDTDRLRGPHEAGSVEDPAAATARKARISADVP
                     AKRLGTPEEFGQICAFLCSVHAGYLTGQNIPVDGGLYVSAF"
     gene            complement(4186..5169)
                     /gene="dgcA"
                     /locus_tag="VGM46_09560"
     CDS             complement(4186..5169)
                     /gene="dgcA"
                     /locus_tag="VGM46_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006206088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-D-Glu racemase DgcA"
                     /protein_id="HEY4192878.1"
                     /translation="MARVISVETERFPIDGVFTISRGSKTEAEVIGCMIADGGHQGRG
                     ECVPYRRYGETMESVLAQIDAMRTKIAAGMGRDALLAAMPAGAARNAIDCALWDLESK
                     ASGVAVAVRIGAAQNPLETAYTLSLGAPEAMAAQARANAARPLLKVKIGGGEGDMDRI
                     RAVREAAPSCRLILDANEGWIDENIEPNMAFAAALGVALIEQPLPVGRDGILRHIAHP
                     VPICADESVHIAQDLVSLAGLYDAVNIKLDKAGGLTAALELRDEARRLGFAIMVGCMV
                     GTSLAMAPAVLLAQGADFVDLDGPLLLARDRTPGLTYNGSLVSPPSRELWG"
     gene            5248..6432
                     /locus_tag="VGM46_09565"
     CDS             5248..6432
                     /locus_tag="VGM46_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019858347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4192879.1"
                     /translation="MLTIGKRDASNTAAATSHQPRIAVRVEDGVLGCAFSGNWTTRTI
                     ASVDAEMRKLEMRDGCRTLALDLSGVDKIDTAGAWLIDRLAGAFQRKGVEVQMQGQSK
                     IASILLDAVNEAGKREKDVAPDRVSNIVIRALAAVGRRVYEMRDDFLAAMNILGATIS
                     GAQMKLGRGHGVNPAAIFNQIDRMGVGAIPVVVLMSAIVGAIVAQQGAYQLSYFGADI
                     FVVDLVGVLVLREMGVLMTAIMIAGRSGSAITAEIGSMKMREEVDALKVIGLNPIGVL
                     VFPRLVALVIALPCLTIIANFAALGGGMAAAYFYSGIPPAAFIDRLRAAIDISTIFAG
                     LIKAPFMAMIIGTIASVEGMKVGGSAESLGTHVTASVVKSIFVVIILDGLFAMFYAAI
                     EF"
     gene            6434..>7144
                     /locus_tag="VGM46_09570"
     CDS             6434..>7144
                     /locus_tag="VGM46_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007764935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY4192880.1"
                     /translation="MPDAEHMPEAANDDGRGEIVLSAHDITVAFSGKTILEGLSLDIR
                     RGEILGFVGASGAGKSVLLRTILDLNKKQSGTIKLFGVDVEKASDLDRLRIDMRLGVL
                     FQHGALFSSLTVMENVQVPMREYLDLPKKLMDELAILKVELVGLPPDAARKFPSELSG
                     GMTKRAALARALALDPDIVFLDEPTSGLDPISAAEFDELVVKLRDTMDLTVYMVTHDL
                     DSLFTACDRIAVLGNKKIL"
BASE COUNT         1300 a         2247 c         2243 g         1354 t
ORIGIN      
        1 ttcgcaattt ttctggtcgc gctcgaagtc tcgaagcgac gccgcattcg acatcgggaa
       61 gaaatagccg tcgcaggttc gcacgcacat ggtgcgaaac tcgccttggg gatgcggtgg
      121 gttttcgagc gtcaatcccg ctaacggctc gatgtccacc ttgccgttgg atatgttttc
      181 catgtcctgg actccggcct ggcggtcgtc gttgcccgca tcgccgtcca gcatgtgctg
      241 tgtggcagcc ggcttttcca gcgtgcggat ggtgattggc tgcttgtcgc ggcaaccacg
      301 agcatcgagc gaagccagta tgcgcgcgcg gtcgcggcgt ccgccacctc cggcaagcct
      361 ggtgcgccgg ctgcgcagcg tatcgagatt atggttcatg cgttcgatgg aggcattgag
      421 cgccgcgcag gccgcaacgt ggccgccgaa cagggaaaat ccgcaccccg cacctcgcgc
      481 ccggctgcgc gccttggcca tctcgtcatt ttgcctggcg atggcggcat cgtattttct
      541 gacttgcgca gggttttctc gtccgccgcc gccggccagt tcggcctcca gttggcggca
      601 tgcccgcgat gccgccattg cctggctggt tgcggcgcac gataaggcga tgacgccaag
      661 caacaaagcg cgatacgcca gcttgccagc cccgcccttc atttgcctct ccgtcatgcc
      721 gctaaacctt gcgtcgccgg tgtacctgga gacccggacg gcgcgttaag cgcggaatct
      781 atcgcggcag cgttaatcgt cccttagaac gcttcacgtc tggcgggaaa ttcgcgttgg
      841 ctcggcggca accgcctgtg ctttctgagt cgcttcgaca acggcgagcg ccgtcatgtt
      901 gacgacgccg cgcgaggtta ccgatggcgt caggatgtga gccggcttgt cggtgccaag
      961 caggatcggc ccgacatgca gcgcgtcgag catcgtctgc aacgcggtca gcgtgatgtt
     1021 ggcggcgtcg aggcttggga acaccagcag attggcctcg cccttcagcc gcgaatgcgg
     1081 gtagacgcgg tggcgaagct gttccgacag cgcggtatcg gcatgcattt cgccgtcgca
     1141 ttgcagttcc ggcgccagcc gtgcgagaat atcggcggcc ttgcgcattt tcagggcgct
     1201 cggcgtatcg cgcgaaccaa agtcggatag cgacaacaag gcagcccgcg gctcgatgcc
     1261 gaagcgccgg atttcctctg ccgcaaggat cgtcatctcg gcgatctgtt cggcggtcgg
     1321 atcgacgcag acatgcgtgt cggtcaggaa caacacgcca cgctgcgaaa tcagcatcga
     1381 aagtgcagaa aattcgtaat ccttgacgcc catccgcggc ccgatgatga ggtcgacatt
     1441 gcgcagatgg cgcgcaaaac gcccttccag cccgcatagc atggcatcgg catcgccacg
     1501 cttgagcgca agggaggcga tgaccgtcgt atgggtgcga accatggtgc gggcggcttc
     1561 ctgcgtgatc ccgcgacgac cgccaagctc gatgaacagg tcgacataat tgcgatagcg
     1621 cggatcgtct tccgggttga tcagctcgaa atccacgcct ggcctgatgc gcagcccatg
     1681 gcgttcgagc cggacatcca cgacatgcgg acgcccgatc aggatcggct gggcaatgcc
     1741 ttcctcgatc accacctggg cggcgcgcag cacgcgttcg tcctcgccat cggcatagat
     1801 gacccgcttg gtagccgatg ccttggccgt cgagaacacc ggcttcatga ccagtccgga
     1861 gcggaagacg aagcggttca gcttgtcgat ataggcgggg aagtcggtaa tcggccgcgt
     1921 cgccacgccg gtttcgcaag cggccctggc gacggcgggt gcgatgcgca ggataaggcg
     1981 cgggtcgaac ggtgagggga tcaggaagtc cggtccgaaa accggcgtct cgccggaata
     2041 ggcgcgggcc gccacgtcgg aaggttcctc acgcgccagc gccgcgatgg cgcgtacggc
     2101 cgccatcttc atgtcctcgt tgatggcgct ggccccgcag tccagcgcgc cgcgaaagat
     2161 gtaaggaaaa caaagtacgt tgttgacctg attgggaaaa tcggacctgc cggtgcagat
     2221 catcgcatct ggcctggctg cgcgggcaag gtccggcatg atttcgggat tggggttggc
     2281 caatgcgagg atgagcggtt tttccgccat cagcttgagc atttcgggct tcagcacgcc
     2341 tgccgccgaa aggccgagga aaacgtcggc gtcgggcaag acatcggcca gcgtacgggc
     2401 gtccgtatcc ttgacatacg ggtccttcca gcggtccatt tcctccgtgc gccccttgta
     2461 ggcgacgccg aagcggtcgg tgacccagat gttttcaatg cgcacgccaa gcaggacaag
     2521 cagattgagg caggcaagcg ctgcggcgcc ggcgccggag gtgacgatct tgatgtcctg
     2581 gatggacttg cccgcgaatt ccagcccgtt caaaatggcc gccgcaacga tgatcgccgt
     2641 gccgtgctga tcgtcgtgga aaacgggaat gcccatcctt tccttcagcc gctcctcgac
     2701 ctcgaagcac tccggggcct tgatgtcttc gaggttgatg ccgccgaaag taggctccag
     2761 cgccgcgatc gtctcgacca tgcggtcgat ttccggcgcg tcgatctcga tgtcgaacac
     2821 gtcgatgccg gcgaatttct tgaacaggac ggccttgcct tccatgaccg gcttggaggc
     2881 gagcgggccg atattgccga gaccaagcac cgccgtgccg tttgaaacca cgccaacgag
     2941 gttggcgcgc ccggtatagt cggcggcggt cgcggggtcg tcgcgaatgg caaggcatgg
     3001 cgctgcaacg ccgggcgaat aggcaagcgc caggtcccgc tggttgccca gcggtttggt
     3061 ggcctggatt tcaagcttgc cgggaaccgg atgcttgtgg aaatggaggg ccgcttcgtc
     3121 cagatcggac catttcgcgt tcgtgctttc gccttccatg gccaaattcc cttaattcca
     3181 agatcacccc ttctagcatg cggcggcagc ttttcgaggc gacaaatgac gacgcgatga
     3241 tcgatttatc atcacgcgaa agtgaatatc ttcaactatt tacgaggcgg tccgcaatga
     3301 ctcgaaccgg cggaacaatg cctagaatgc gctgacataa aggccgccat cgacgggtat
     3361 gttctggccg gtcaaatagc cggcgtgaac cgaacacagg aaggcgcata tctggccgaa
     3421 ctcctccggc gtgccgagcc gttttgccgg aacatcggcg ctgatgcggg ccttgcgcgc
     3481 cgtggccgcc gcagggtctt ccacgctccc cgcttcgtgc gggccacgca ggcggtcggt
     3541 atcgagcttg ccgggcagca tgctgttgat ggtgacgttg cggtccatga cggtgcgcgc
     3601 cacgccggcg agaaacgagg tcaggccggc gcgcgcgcca gacgacaggt caaggccagg
     3661 aatgggcacg tagaccgaca gcgatgtgat gttgatgatg cggccgaaac cgcgagcggc
     3721 catgccgtcg atcacgactt tgaccagttc gataggggtg accatattgt tggtgaggcc
     3781 ttcgagaatc ttttcccggt caagctcacg gaaatcccgg aacggcgggc cgccattgtt
     3841 gttgacgaga atatccggcg cgggacatgc ggcgagcagg gccttctgga catcgggctt
     3901 cgatacgtcg ccgacgatct cgatcacctc gacgccgaat ttttcgcgca gctcggccgc
     3961 ggtcttcgcc agtaaggcgg cgttgcggcc gttaatgaca aggtcgcatc ccgcctcggc
     4021 cagggcagcc gcgcagccgc gccccagccc cttgctcgat gcgcaaacaa tggccttctt
     4081 cccgcgaatg ccgaggtcca tggctaaatc ctccagtgat gagcctgcca agctaaccgc
     4141 caacgcgaca gccagcaact acgatctatc cagccaagcg cttgactagc cccataattc
     4201 tcgggaaggc ggcgacacca gcgatccgtt ataggtcagg ccgggcgttc ggtcgcgcgc
     4261 cagcagcagc ggcccgtcga gatcgacgaa atccgcgccc tgcgccagaa gaaccgccgg
     4321 cgccatcgcc agcgaagtgc cgaccatgca cccgaccatg atggcgaagc cgagacggcg
     4381 ggcctcgtcg cgcagttcga gcgccgccgt cagcccgcct gccttgtcga gcttgatgtt
     4441 gacggcatcg taaaggccgg caagcgagac caaatcctgc gcgatgtgga cgctttcatc
     4501 ggcgcagatc ggcacggggt gcgcgatatg ccgtaagatg ccatcgcgcc cgacaggcag
     4561 cggctgctcg atcagtgcca cgccaagcgc tgccgcgaac gccatgttcg gttcgatatt
     4621 ttcgtcgatc cagccttcat tggcgtcaag gataagccgg caggaaggcg cagcctcccg
     4681 cacggcccgg atgcgatcca tgtcgccttc gccgccgccg atcttcacct tgagcagcgg
     4741 ccgggcggcg ttggcgcgcg cttgcgccgc catcgcctcc ggcgcgccaa gcgacagcgt
     4801 ataggcggtt tccagcgggt tttgcgccgc gccgatgcga accgccacgg caacgccgct
     4861 ggctttgctt tccaggtccc ataaggcgca gtcgatggcg ttgcgcgcgg cgcccgccgg
     4921 catggcggca agcaaggcat cgcggcccat cccggcggca attttggtgc gcatggcgtc
     4981 gatctgcgcc agcacgcttt ccatcgtctc gccatatcgc ctgtagggca cgcattcgcc
     5041 tcggccctga tggccgccat ccgctatcat gcaaccaatc acctcggctt ccgttttgga
     5101 gccgcgagaa atggtgaaga caccgtcgat cgggaaccgc tctgtctcga ccgaaatgac
     5161 acgcgccata tttttcttct ccggccatgc gactacagag gcttcgccaa atcgacagcc
     5221 ccgattcgcc gggctaaaca ggaagtcatg ttgaccatcg gcaaacgcga cgcaagcaac
     5281 accgccgccg ccaccagcca ccagccgcgc atcgccgtgc gtgtcgagga tggcgtgctc
     5341 ggctgtgcct tttccggcaa ctggacgacg cgcaccattg cttccgtgga cgcagaaatg
     5401 cgcaaacttg aaatgcgcga tggctgccgg acgctggcgc ttgacctttc cggggtcgac
     5461 aagatcgaca cggccggcgc atggctgatc gatcgcctgg ccggcgcttt ccagaggaaa
     5521 ggcgtcgagg tccagatgca ggggcagagc aagattgcct cgatcctgct tgatgccgtg
     5581 aacgaggcgg gaaagcgcga aaaggacgtc gcgccggacc gggtttcaaa catcgtgatt
     5641 cgcgcgctgg cggctgtcgg ccggcgcgtt tacgaaatgc gcgacgactt cctggcggca
     5701 atgaacattc tgggcgcaac gatcagcggc gcgcagatga agcttggccg tggccacggc
     5761 gtcaatccgg ccgcgatctt caaccagatc gaccggatgg gcgtcggcgc cattcccgtg
     5821 gtggtgctga tgtcggcgat cgtcggcgcc atcgtcgccc agcagggcgc ctatcagctc
     5881 agctactttg gcgccgacat tttcgtcgtc gatctggtcg gcgtgctggt gctgcgcgaa
     5941 atgggcgtgt tgatgacggc gatcatgatt gccggccgat ccggcagcgc gatcaccgcc
     6001 gagatcggct cgatgaagat gcgcgaggag gtcgacgccc tcaaggtcat cgggctgaat
     6061 cccatcggcg tgctggtgtt cccgcgcctg gtggcgctgg tgattgcctt gccttgcctt
     6121 accatcatcg ccaatttcgc agcgctgggc ggcggcatgg cggcggccta tttttattcc
     6181 ggcatcccgc ctgccgcgtt catcgaccgc ctgcgcgccg ccatcgacat cagcaccatt
     6241 ttcgccgggc tgataaaagc cccgttcatg gcgatgatca tcggcaccat cgcctccgtc
     6301 gaaggcatga aggttggcgg cagcgcggaa tcgctgggaa cgcacgtcac cgcatctgtc
     6361 gttaaatcga tcttcgtcgt catcatcctc gatggcctgt tcgccatgtt ctatgcagca
     6421 atcgagtttt gacatgcctg acgccgaaca catgcccgaa gcggcgaacg acgacgggcg
     6481 gggcgagatc gtcctgtcgg cgcatgacat taccgtcgca ttttccggca agactatcct
     6541 tgagggtctg tcgctcgata tcaggcgcgg tgaaatcctt ggctttgtcg gggcctccgg
     6601 cgccggcaaa tcggttctgc tgcgcaccat cctcgacctc aacaagaagc agtcgggcac
     6661 catcaagctg ttcggcgtcg acgtggaaaa ggcgagcgac ctggaccgcc tcaggataga
     6721 catgcgcctt ggcgtccttt tccagcatgg cgctttgttt tcctcgttga ccgtcatgga
     6781 aaacgtccag gtgccgatgc gcgaatatct cgacctgccc aagaagctca tggacgaatt
     6841 ggccatactc aaggtcgaac tggtcggcct gccgcccgat gcggcgcgca agtttccgtc
     6901 cgaactgtcc ggcggcatga ccaagcgcgc cgccttggcg cgcgcattgg cgctcgaccc
     6961 cgacatcgtt tttctggacg agccgacatc gggcctcgac cccatcagcg cggcggaatt
     7021 cgacgaactg gtggtcaagc ttcgcgacac gatggacctg accgtctaca tggtgaccca
     7081 cgacctcgat tcgctcttca ccgcctgcga ccgcatcgcc gtgctcggca acaagaagat
     7141 actg
//
LOCUS       DASYCS010000305         5468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1967800,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000305 DASYCS010000000
VERSION     DASYCS010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 5468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5468
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1967800"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..499
                     /gene="nusB"
                     /locus_tag="VGM46_09575"
     CDS             32..499
                     /gene="nusB"
                     /locus_tag="VGM46_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010915597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEY4192881.1"
                     /translation="MRQANKRGAARLAAVQALYQMDVAGSGVFEITAEYEAFRLGKEV
                     DGALYREADAQWFRAILAGVVENQKTVDPIIRQALTEDWPLSRLDSTLRAILRAGVFE
                     LMKRADVPTAVIVSEYVDIAKAFYTEDEPKLVNAVLDRVSRRVRGEGRGKDVS"
     gene            496..1701
                     /locus_tag="VGM46_09580"
     CDS             496..1701
                     /locus_tag="VGM46_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4192882.1"
                     /translation="MIAATPTEARRTALVLSAAQAVLGSAGPICFAMGALAGYFMLGA
                     DKSLATAPLTGYSVGLAVGALPAAAIIRRLGHRDGFMTGAGVAALGGLIAAIALFQSS
                     FWLFAFGLAVIGSGNAFVQQFRFAAADNAPPEFKARAISMVLAGGIVTAVLGPQTVIF
                     THDLLAPVPFAGSFVAIIALSAIGAAILFFLPTRTIVKTQQANVDTGRPLLEIISQPR
                     YAVALLCAVGSYSMMTFVMTGAPLAMVGCGFSSDDATLGISWHVMAMFAPSFFTGRLI
                     HRFGAGAVVTCGFALLIACSLVALSGIQLWQFWTALILLGLGWNFGFIGSTAIVASCY
                     RAEEKGKAQGFHDFALFSCVAFASLMSGTVYNAWGWEMLAVIVMPIAGLCLVMLGVQH
                     LANRRVQVS"
     gene            1846..2760
                     /locus_tag="VGM46_09585"
     CDS             1846..2760
                     /locus_tag="VGM46_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002968686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY4192883.1"
                     /translation="MRSLGLFAGAALALSISIASSLAAPVVVSSKIDTEGGVLGSIIE
                     QVLNANKIETADRIQLGGTPVVRKAIIAGEIDIYPEYTGNAAYFFEKTADPVWKNAAQ
                     AYEAAKKLDYDANKIVWLSPSPANNTWAIALRKEVVDQNKLKTLSDLGKYVAGGGQVV
                     LAASAEFVNSEAALPAFQKAYGFTLKPEQLITLSGGDTAATIAAAANQTNGANAAMVY
                     GTDGGIAPSNLVVLDDDKGVQPVYQPAPIIREAVLKEHPEIEALLKPVFAKLDLVTLQ
                     ELNGRVQVGGEPVKGVAEDFLKKNGFLK"
     gene            2783..3946
                     /locus_tag="VGM46_09590"
     CDS             2783..3946
                     /locus_tag="VGM46_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006332624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4192884.1"
                     /translation="MRFDKLGVVIAVIAAYGALVAPFATFRANRIVPGQARLILEALP
                     TPAALVLTAVILAAALIALFKTPLWLRLAASVVSLATLALLIGVAGSYLTPEGNTFAR
                     VAPAHGFWLLVFAFALLLADVLTRLKLSPMARIGVLVLAALAAAMLLLSGSWNGLSIL
                     KEYFNREDSFWTEAGKHMVLALGSLVAAVLVGLPLGILCHRVEKLRAGVLNVLNIIQT
                     VPSIALFGLLIAPLGWVAAHVPGASAIGIRGIGAAPAFIALFLYSLLPVVANTVVGLA
                     GVPRAANDAARGMGMTDRQRLLGVEFPLAFPVILTGIRIVLVQNIGLATIAALIGGGG
                     FGVFVFQGVGQTAMDLVLLGAVPTVALAFAAAIILDAVVELTSMRTRREENPA"
     gene            3943..4881
                     /locus_tag="VGM46_09595"
     CDS             3943..4881
                     /locus_tag="VGM46_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4192885.1"
                     /translation="MIEIDAVTKRYDGTAVVDNVSMMVKPRTICAVVGTSGSGKTTLL
                     RMINRLVEPSEGSIRLDGVDNRSMPAFELRRRIGYVIQGYGLFPHRTVAQNIATVPTL
                     LGWPAARIDARVHELLMLFQLDPAAFGPRYPHELSGGQQQRVGVARGLAAEPNVLLMD
                     EPFGALDPIIRTKAQEDLLAIQKRFGTTIILVTHDMEEAVHLGDMIAVMDAGKLVQYA
                     KPAEILARPANAFVETLVGVSERPFRLLSLGRVADAVEKGAAEGEAIGADASQRDALA
                     ELMWSGRPALPVSDAQGKALGRVTVKGLLKHAVKPA"
     gene            4878..>5468
                     /locus_tag="VGM46_09600"
     CDS             4878..>5468
                     /locus_tag="VGM46_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4192886.1"
                     /translation="MKWLPALLRLALLVLLVAFVTEPAWFEPLLKPLTENNAPAIYNQ
                     GSLLSLTYLHLRTVMIATVAAIIVAVGLAILVTRPAGAEFLPLSRSLVNIGQTFPPVA
                     VLALAVPAVGFGEKPTLIALFLYGLLPVFENALTGLTNLPANVVEAAKASGMTSWQRL
                     IKIELPLSLPVILAGIRLSVVISLATATIGSTVAAKT"
BASE COUNT          860 a         1756 c         1749 g         1103 t
ORIGIN      
        1 cgtgagcgac accgaaccca ccggcccggt catccgtcaa gccaacaagc ggggcgcggc
       61 ccggcttgcc gccgtgcagg cgctctacca gatggatgtc gccggcagcg gcgtgttcga
      121 gatcaccgcc gaatacgagg cgttccggct cggcaaggaa gtggacggcg cgctctatcg
      181 cgaggccgac gcgcaatggt tccgcgccat ccttgccggc gtggttgaaa accagaagac
      241 cgttgatccc attatccgcc aggcgctgac ggaagactgg ccgctgtcgc ggctggactc
      301 gacattgcgc gccatcctgc gcgccggcgt gttcgagctg atgaagcgcg ccgacgtgcc
      361 gaccgccgtc atcgtctcgg aatatgtcga tatcgccaag gcgttttaca ccgaggacga
      421 gccgaagctg gtcaatgccg tgcttgaccg cgtatcgcgc cgggtgcgcg gcgaagggcg
      481 aggcaaggac gtgtcgtgat cgccgccacg cccacggagg cgcgccggac cgcactggtc
      541 ctttcggcgg cgcaggccgt actcggctcg gcggggccga tctgctttgc catgggcgcg
      601 cttgccggtt acttcatgct gggcgccgac aagtcgctgg cgaccgcgcc gctcaccggc
      661 tatagcgtcg gcttggctgt tggcgctttg ccggccgccg cgatcatccg gcggcttggc
      721 caccgcgatg gcttcatgac cggcgccggc gtggcggcgc tcggcggctt gatcgccgcc
      781 atcgcactgt tccagtcgag tttctggcta ttcgccttcg gccttgccgt gatcggttcc
      841 ggcaatgcct tcgtgcagca gttccgtttc gccgccgccg acaatgcgcc gcccgaattc
      901 aaggcgcgcg ccatttcaat ggtgctggcc ggcggcatcg tcacggcggt gctgggaccg
      961 cagaccgtca tcttcacgca tgatttgctg gcgccggtgc cgttcgccgg ttcgttcgtc
     1021 gccatcatcg ccttgtcggc gattggcgcg gccatcctgt ttttcctgcc gacccgcacg
     1081 atcgtcaaaa cgcagcaggc caatgtcgac accggccggc cgctgctcga aatcatttcg
     1141 cagccgcgct atgccgtggc gctgctatgc gccgtcggtt cctactcgat gatgaccttt
     1201 gtcatgacgg gcgcgccgtt ggccatggtc ggttgcggct tctcctccga cgacgccacg
     1261 cttggcatct cgtggcatgt gatggcgatg tttgcgccaa gcttcttcac cggccggctc
     1321 atccaccgct tcggggcggg cgccgtggtg acgtgcggct tcgcattgct catcgcctgt
     1381 tcgctggtgg cgttgtcggg catccagctt tggcagttct ggaccgcgct gatcctgctt
     1441 ggccttggct ggaatttcgg cttcatcggc tcaaccgcca tcgtggcctc atgctaccgc
     1501 gccgaagaaa agggcaaggc gcagggtttt cacgacttcg cgctgttttc atgcgtcgcc
     1561 ttcgcctcgc tgatgtcggg caccgtctat aacgcctggg gctgggaaat gctggccgtc
     1621 atcgtcatgc cgatagccgg gctttgcctg gtgatgctgg gtgtccagca tctggcgaac
     1681 cgccgggtgc aggtctcctg acaacccacg gtcagcatga gatggcgtgt ttgaacaaac
     1741 cgattgcttt aatcccgcat gttatgaaac gttatgctcc gaccggcctt tgaagctgtg
     1801 ctatatgcag ccgcctagtt tcagttccat caaggggatt tcaaaatgcg ttcactcggg
     1861 cttttcgctg gcgcagcgct cgcgctcagt atttcgatcg cctccagcct tgccgcgccg
     1921 gtggtcgttt cctccaagat cgacaccgaa ggcggtgtgc tgggcagcat aatcgaacag
     1981 gtgctgaacg ccaacaagat cgagacggcc gatcgcatcc agcttggcgg cacgcctgtg
     2041 gtgcgcaagg cgatcatcgc cggcgagatc gatatctatc cagaatatac gggcaatgcc
     2101 gcctacttct tcgaaaagac ggccgatccg gtctggaaga acgccgcgca ggcctacgaa
     2161 gccgccaaaa agctcgacta cgacgccaac aagatcgtct ggctgtcgcc atctccggcc
     2221 aacaacacct gggcgattgc cctgcgcaag gaagttgtcg accaaaacaa gctcaagacg
     2281 ctcagcgatc tgggtaaata tgttgccggc ggcgggcagg tcgtattggc ggcctcggct
     2341 gaattcgtga attcggaagc ggccttgccg gccttccaga aggcttatgg ctttacgttg
     2401 aagcccgagc agctgatcac gctctccggc ggcgatacgg cggcgacgat tgcggccgcc
     2461 gccaaccaga ccaacggcgc caatgccgcc atggtctacg gcacagatgg cggcattgcg
     2521 ccgtcgaacc tcgtcgtgct cgacgacgac aagggcgtgc agccggtcta ccagcccgcg
     2581 ccgatcatcc gggaggccgt cttgaaggag cacccggaaa ttgaggcgct gctaaagccg
     2641 gtgtttgcca agctcgacct cgtcacgttg caggagctga acggtcgcgt ccaggtcggc
     2701 ggcgagcctg tcaaaggcgt ggccgaagat ttcctgaaga aaaacggctt cctgaaatag
     2761 ccgcccttgg ggtttcggcg ccatgcggtt tgacaagctg ggtgtggtta ttgcggtgat
     2821 cgcagcctat ggcgccctgg tggcgccatt tgcgacgttt cgcgccaacc gtatcgtgcc
     2881 cggccaggcc cgcctgatcc ttgaagcgct gccgacgccg gccgccctcg ttttgacggc
     2941 cgtgatcctt gccgccgccc tcatcgccct tttcaaaacg ccgctgtggc tgcgccttgc
     3001 agccagcgtc gtttcgctgg cgacgctggc gctgctcatc ggcgttgccg gcagctacct
     3061 cacccctgaa ggaaatacgt tcgcccgcgt cgcacccgcc cacggcttct ggctgctggt
     3121 cttcgccttc gcgctgctgc tggccgatgt gctgacccgg ttgaaacttt cgccgatggc
     3181 gcggataggc gttctggttc tggcagcgct ggcggccgcc atgctccttt tgtctggaag
     3241 ctggaacggc ctttccatcc tgaaggaata tttcaaccgc gaagacagtt tctggaccga
     3301 ggccggcaag cacatggtgc tggcattggg ttcgctggtc gcggcggtgc tggtcgggct
     3361 gccgctaggc attttgtgcc accgcgtcga aaagctgcgg gccggcgtgc tgaatgtgct
     3421 caacatcatc cagaccgttc cttcgattgc gcttttcggc ttgttgatcg cgccgcttgg
     3481 ctgggtcgcg gcgcatgtgc cgggcgcttc cgccatcggc atccggggca tcggcgccgc
     3541 gccggccttt atcgcgctgt tcctctattc gctgctgccg gtcgtcgcca acaccgtggt
     3601 tggccttgcc ggcgtgccgc gcgcggccaa cgatgcggcg cgcggcatgg gcatgaccga
     3661 caggcaacgc ctgctgggcg tcgagtttcc gctagccttt ccggtgattc tcaccggcat
     3721 ccgcatcgtt ttggtgcaga atatcggcct tgccacaatc gccgcgctga ttggcggcgg
     3781 cggcttcggc gtcttcgtgt tccagggcgt cggccagacg gcgatggacc tcgtgctgct
     3841 tggcgcggtg ccgacggtgg ctctggcctt cgccgccgcc atcatattgg acgctgtcgt
     3901 cgaattgacc agcatgcgca cgcggcgcga ggaaaacccc gcatgatcga aatcgacgca
     3961 gtcaccaagc gctatgacgg cacggctgtc gtggacaacg tctcgatgat ggtgaagcct
     4021 cgaaccatct gcgccgtcgt cggcacgtcc ggctcgggca agacgacgct tttgcgcatg
     4081 atcaaccggc tggtcgagcc gagcgaggga tcgatccggc tggacggcgt cgacaaccgt
     4141 tcgatgccgg ctttcgagct gcgccgccgc atcggctatg tcattcaggg ctatggcctt
     4201 tttccgcacc ggaccgtggc gcagaacatc gccaccgttc ccacgctgct cggctggccc
     4261 gccgcccgca tagacgcgcg cgtgcacgaa ttgctgatgc tgttccagct tgatccggca
     4321 gccttcggtc cccgctatcc gcatgaactg tcgggcggcc agcaacagcg tgtcggcgtt
     4381 gcgcgcggat tggcggcgga gccgaacgtc ctcttgatgg acgagccttt cggcgcgctc
     4441 gacccgatca tccgcaccaa ggcgcaggaa gatctcctgg ccatccagaa gcgttttgga
     4501 acgacgatca tccttgtcac ccacgacatg gaggaggccg tccacctcgg cgacatgatc
     4561 gcggtgatgg atgcgggaaa gctggtgcaa tatgcaaagc cggccgagat attggcgcgc
     4621 ccggcgaatg ccttcgtcga aacgctggtc ggcgtcagcg aacggccgtt ccggctgctc
     4681 tcgcttggcc gggttgccga cgcggtggaa aagggcgcgg ccgagggcga ggcgatcggc
     4741 gccgacgcca gccagcgcga cgccttggct gaactcatgt ggtccggccg gccggcattg
     4801 ccggtcagcg atgcgcaggg caaggctttg gggcgcgtca ccgtgaaggg tctgctcaag
     4861 cacgcggtca agccggcatg aaatggcttc ccgccttgct gcggcttgcg ctattggtgc
     4921 tgctggtcgc cttcgtgacg gaaccggcat ggttcgagcc gctcttgaag ccgctcaccg
     4981 aaaacaacgc gcccgccatt tacaaccagg gcagcctgct gtcgctgacc tatctgcatc
     5041 tgcgaaccgt gatgatcgcg acggttgccg cgatcatcgt cgcggtcggc ctggccatcc
     5101 tggttacacg gccggccggc gcggaattcc tgccgctgtc gcgcagcctc gtcaatatcg
     5161 gccagacttt cccgcccgtg gcggtgttgg cgctggccgt gcccgccgtc ggcttcggcg
     5221 aaaagccgac gctgatcgcg ctgtttctgt acgggctgct gccggtcttc gaaaatgcgc
     5281 tgaccggcct aaccaacctg ccggccaatg tcgtcgaggc ggcgaaggcc tccggcatga
     5341 cgagctggca gaggttgata aagattgaac tgccgcttag cctgccggtt attctggcgg
     5401 gcatcaggct gtcggtggtg attagcctcg ccacggcgac catcggctcg actgtcgcgg
     5461 caaagacg
//
LOCUS       DASYCS010000306         7054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3272469,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000306 DASYCS010000000
VERSION     DASYCS010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 7054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7054
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3272469"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="VGM46_09605"
     CDS             <1..847
                     /locus_tag="VGM46_09605"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872264.1"
                     /note="catalyzes the DNA-template-directed extension of
                     the 3'-end of a DNA strand; the tau chain serves as a
                     scaffold to help in the dimerizaton of the alpha,epsilon
                     and theta core complex; the gamma chain seems to interact
                     with the delta and delta' subunits to transfer the beta
                     subunit on the DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEY4192887.1"
                     /translation="AMAKALSVRVLSRTWQMLLKGIPEVQGSSRPVSAAEMVLIRLAH
                     AADLPTLDEALKTLEGGTFSPAPEPRTNGAPISAAPASFNSLSSSSFNDSGPAPQARM
                     TASGGGAQAMRLVAATPAPEPASFIAPPEPAAAEQPLVPVKSLADIAALADTHRDVAF
                     KVLLKRCVRPVRIEPGRIEVSLTADAPKLLLNDLNSRLRSWTGRNWLVSLSKDEGGQT
                     LAEMESAKRETAFLDARSDPAVAAVLARFPGAKIIDVRIPDSPDAEISETDLPPEPPA
                     DDDDN"
     gene            881..1204
                     /locus_tag="VGM46_09610"
     CDS             881..1204
                     /locus_tag="VGM46_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010913283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEY4192888.1"
                     /translation="MKDLLGLMGKAKEMQAKFQAMQEEIASVEASGQAGGGLVEVTLG
                     GKFDMKALKIDPSLFKEDQVEILEDLILAAHNDAKGRIEQVMQEKTQALTAGLPIPPG
                     MKLPF"
     gene            1419..2414
                     /locus_tag="VGM46_09615"
     CDS             1419..2414
                     /locus_tag="VGM46_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013527929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HEY4192889.1"
                     /translation="MPTLLGILVFPFLLAGCSQTTNSISAAGLTDSLTPGFLRARPYR
                     YTSRDKECLERAMFFESNRSSREGMIAVGTVVMNRLRSGQHGSTVCAVVGERRQFAPG
                     VMTRRMNSRAMPDVEAAAESVLRGERAPKLKNAMHFHTAGLKFPYKNMHYVEVAGGNA
                     FYEKRGRNWKPLPPETTTMVAFAGPAPATAATAAPTMVASAEPAAVALPVISSASSPV
                     VASFNSSGQMAAGETSNATAALAASAPVPTPRTVAMAAPQSGKQDRAVQLAMQEPDNA
                     RFGASDFDTAKKSAPVMAAAVQNDAQQPAMSFQSTPDDTNAIGALIMSESRPMNE"
     gene            2476..3081
                     /gene="recR"
                     /locus_tag="VGM46_09620"
     CDS             2476..3081
                     /gene="recR"
                     /locus_tag="VGM46_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010913286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEY4192890.1"
                     /translation="MSKRIAGPEIERLIQLLAKVPGLGPRSARRAALHLIKKKEQLLQ
                     PLAHAMGEAVDKVRICSTCGNVDTADPCMICTDPRRGNDALIVVEDVADLWALERATA
                     MNVRYHVLGGTLSPLDGIGPDQLNIRSLVDRVALGEIKEIILAVNATVEGQTTAHYLT
                     DQLADFDVKITRLAHGVPVGGELDYLDEGTLAAALKSRTAF"
     gene            3183..4376
                     /locus_tag="VGM46_09625"
     CDS             3183..4376
                     /locus_tag="VGM46_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015314041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEY4192891.1"
                     /translation="MVLALPASAARIEDQFQKWLQADLWPQAKAEGISEKTFDAAFAG
                     VKPNLKLPDLVIPGEKPTVPKKQHQAEFGSPGAYFAEKTIGAVTSGGRARAAANARTL
                     AAIEKKYGVPGEILLAIWGRETGYGAAKLSYDAFEVLGTKAFMSTRKDMFRTEVLAAL
                     QIVQKGLAARAAMKSSWAGALGQPQFMPSSFLKEAVDFDGDGHPDIWNSTPDVLASIA
                     SYLVDYGWVKGRDWGFEVTVPAGISCSLEGPDQGKRISQWAQMGIERIGGKPFPAAEA
                     RAQGFLLMPAGREGPAFIVTPNFYVLKEYNMSDLYALFIGHSADRIAHGDRRFTAGWG
                     AVGGLYRSDVAALQRALEKKGYDVGSADGLPGFKTRRSIGAWQEKNGRAATCFPDAAM
                     VKALK"
     gene            complement(4441..5442)
                     /locus_tag="VGM46_09630"
     CDS             complement(4441..5442)
                     /locus_tag="VGM46_09630"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY4192892.1"
                     /translation="MATLSVLDLSPVAEGSNPSQSLANSLDLARHAERLGYKRYWLAE
                     HHNMPGIASAATAVVIAHIAGGTESIRVGSGGIMLPNHAPLVIAEQFGTLAALHPGRI
                     DLGLGRAPGTDMLTARALRRNMEAGVDTFPQDVVELIRYFEPAEEGQRIRAVPGEGQE
                     VPVWILGSSLYGAQLAAVLGLPYAFASHFAPAELDHAIEIYRSRFQPSQQLAKPYVML
                     GLNVFAAPTDAEAQLLFTSLQQAFVNLRTGRAGRLPPPVENYDRDLDPMAKTMLGQAL
                     SCAVVGSPETVRQGVEAFLRRTGADELMVTAQIFDHGARVRSFEILAEANRALSQAA"
     gene            complement(5514..6671)
                     /locus_tag="VGM46_09635"
     CDS             complement(5514..6671)
                     /locus_tag="VGM46_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013891838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY4192893.1"
                     /translation="MSTDNTPSSGQVPVASDVRADIRLMRSMLVGLFLFAAVFALYYG
                     RDFFMPVILAFLLALMLTPIVRLLRKHGVPEAISATLLVLLSLGFATTAGYLLSGPAI
                     DLVNNAPSIGQQLSERLDHLRRPFEKLMKITEQIGALTEPSQQPGVSRVVVSQTGIVS
                     QAAGSVLSGVTSITIVFVLSLFLLASGTMFYEKIVQSFTRMSEKKRALRVVYDVEREI
                     SRYLLTVALINAGLGVAVALGLWALGMPNPMLWGATAALLNFLPYIGALMNVVLVGVI
                     ALISFDSIAYAMLAPAFVLLCNIVEGQFVTPMIVGRRLEINSVAIFIAIAFWSWMWGF
                     VGALMAVPLLVVVKVFCDHFDGLRHVGNFLSAQQALVIEEEDKEEAARLTA"
     gene            6894..>7054
                     /locus_tag="VGM46_09640"
     CDS             6894..>7054
                     /locus_tag="VGM46_09640"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862443.1"
                     /note="catalyzes the phosphorylation of
                     N-acetyl-L-glutamate to form N-acetyl-L-glutamate
                     5-phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEY4192894.1"
                     /translation="MTDVAATAAMQAALLSAALPYMQRYENKTVVVKYGGHAMGDTSL
                     GKAFARDIAL"
BASE COUNT         1391 a         2218 c         2263 g         1182 t
ORIGIN      
        1 tgcaatggct aaggccttgt ccgtgcgcgt cctttcgcgc acatggcaga tgctgctgaa
       61 gggaattccc gaggttcaag gctccagccg cccggtaagc gcggccgaaa tggtgctgat
      121 ccgcctcgcc cacgccgccg acctgccgac gctggacgaa gcgctcaaga cgctggaagg
      181 cgggactttt tcgcccgcgc ccgagccgcg caccaacggc gcgccgatct cggccgctcc
      241 cgcttccttc aattccctgt ccagttcctc cttcaacgat agcggccccg cgccgcaggc
      301 acggatgacg gcaagcggcg gcggcgcgca ggcgatgcgg ctggtggcgg ccacgcccgc
      361 gccggaaccg gcaagtttca tcgcgccgcc ggaacccgcg gcggcggagc agccattggt
      421 gccggtcaag tctctggccg atatcgccgc ccttgcggac acgcatcgcg acgttgcctt
      481 caaggtgctt ttgaagcgtt gcgtacgccc tgtccgcatc gagcctggcc gcatcgaagt
      541 cagcctgact gccgacgcgc ccaagcttct gttgaacgac ctcaattccc ggctgcgcag
      601 ttggaccggt cgcaactggc tggtttcgct gtcgaaagac gaaggcggac agaccctggc
      661 cgagatggaa tcggccaagc gcgagaccgc ctttctcgac gcccgcagcg atccggccgt
      721 cgcggccgtg ctggcgcgct ttcccggcgc caagatcatc gacgtgcgca ttcccgattc
      781 gccggacgct gaaatcagcg agaccgattt gccgccggaa ccgccggcgg acgacgacga
      841 caactgaccg caacgacact taccggcaaa ggatcggacc atgaaagatc ttctcggcct
      901 gatgggcaag gcgaaggaaa tgcaggccaa attccaagcc atgcaggaag aaatcgccag
      961 cgtcgaggct tcgggacagg ccggcggcgg cctcgtcgag gtgacgcttg gcggcaaatt
     1021 cgacatgaaa gcgctgaaga tcgacccttc gctgttcaag gaagatcagg tcgaaatcct
     1081 cgaagacctc attcttgccg cccataacga cgccaagggc cgcatcgagc aggtcatgca
     1141 ggagaagacc caggcgctga ccgccggctt gcccataccg ccgggaatga agctgccgtt
     1201 ctgacgccat cttttcgccg ggtcacgcta tgtaacggcg caagacagaa tgttgatcgc
     1261 cttcaagccc ttgggcgccg gcttcaatcg tcattattaa ggcgttagcc atctttttgc
     1321 cggaaactgt ctcattcttg ccatggcaca aggcgggaca gcagcagaac gaggccggtt
     1381 tgacgattta ccacaggttg gtcgctctgc gatggaaagt gccaacgctg cttggcattc
     1441 tggtcttccc gttcctgctg gctggttgca gccagacgac gaacagcatt tcggccgccg
     1501 gcctgacgga cagcctgaca cccggctttc tccgcgcccg cccctatcgc tacacatcgc
     1561 gcgacaagga gtgcctcgaa cgggcgatgt tcttcgaatc caaccggtcc agccgtgaag
     1621 gcatgatcgc ggtcggcacc gtagtgatga accggctgcg ctcgggccag catggcagca
     1681 ccgtctgcgc cgtcgttggc gaacggcgcc agttcgcgcc cggcgtgatg acgcggcgca
     1741 tgaattccag ggcgatgccg gatgtcgagg cggcggccga atcggtgttg aggggcgagc
     1801 gcgcgccgaa gctcaagaac gccatgcact tccacaccgc cgggctgaaa ttcccctaca
     1861 agaacatgca ctatgtcgaa gtggccggcg gcaacgcctt ctacgaaaag cgcggccgca
     1921 actggaagcc gctgccgccc gagacgacaa cgatggtcgc tttcgccggc ccggcgccgg
     1981 cgaccgcagc tacagccgct ccaacgatgg ttgcctctgc cgagccggct gccgtggcat
     2041 tgccggtaat aagctcggct tcgtcacccg tggtcgcttc cttcaacagt tccgggcaga
     2101 tggcggctgg cgaaacttcc aacgcaacgg ccgcgcttgc cgcctcggct cccgtgccga
     2161 caccccgcac cgtggcgatg gccgcgccgc aatcgggcaa gcaggacagg gcggtgcaac
     2221 tggcgatgca ggagccggat aacgcccgct tcggcgcatc ggatttcgat acggcgaaga
     2281 aatcggcgcc ggtcatggcg gcggcggtcc agaacgacgc gcagcagccg gcgatgtcgt
     2341 tccagtcaac gcccgacgat acgaacgcca tcggcgcgct catcatgagc gaaagccggc
     2401 cgatgaacga gtagcaggcc gcctccggcc atcagcctgc ttttcgcccc gcgtacttcg
     2461 ccttatatgg acgtcatgtc caagcgaatc gccggtcccg aaatcgaacg cctgatccag
     2521 ctcctggcca aggtgccggg acttgggcca cgctcggcgc gccgggccgc gctgcatctc
     2581 atcaagaaga aggagcaatt gctccagccg ctggcccatg ccatgggcga ggccgtggac
     2641 aaggttcgca tctgctcgac ctgcggcaat gtcgatacgg ccgacccttg catgatctgc
     2701 accgacccgc gccgtggcaa cgacgccctg atcgtggtcg aggacgttgc cgatctatgg
     2761 gcgctggagc gggcgactgc gatgaatgtg cgctaccatg tgctgggcgg cacgctgtcg
     2821 ccgctcgacg gcatcggccc ggaccagctc aacatccgct cgctggtcga ccgcgtcgcg
     2881 ctgggcgaaa tcaaggaaat catcctggcg gtcaatgcca cggtcgaggg ccagaccacc
     2941 gcgcattacc tgaccgacca gcttgcggat ttcgacgtga agatcacgcg attggcgcat
     3001 ggcgtgccgg tgggcggcga actcgattat ctggacgagg gaacgctggc ggcggcgctg
     3061 aaatcgcgca ccgcgttctg acgcaatttg cgggcaaggc aagggtcgag ggggttcgga
     3121 tgaacgcagg cgcgaccaga ccgaggtttt tcccggcgct cgtggctgct ttgctggcgc
     3181 tcatggttct agccctaccc gcatccgccg ccaggatcga agaccagttc cagaaatggc
     3241 tgcaagccga tttatggccg caggccaagg ccgaaggcat ttcggaaaag accttcgatg
     3301 cggcctttgc cggggtgaag cccaatctga agctgcccga cctggtcatt cccggtgaaa
     3361 agccgactgt gcctaaaaag cagcatcaag cggagtttgg ttcgccgggc gcctatttcg
     3421 ccgaaaagac catcggcgcc gttacttccg gcgggcgggc gagagccgcc gccaatgcca
     3481 ggacccttgc ggcaatcgag aagaaatacg gcgtgccggg cgaaattctg ctggctatct
     3541 ggggccgcga gaccggctat ggcgcggcga aactatccta tgacgccttc gaggtgctcg
     3601 gcaccaaggc gttcatgtcg acgcgaaagg acatgttccg caccgaggtg ctggcggcgt
     3661 tgcagatcgt ccagaagggc ctggccgcgc gcgcggcgat gaaatcgtcc tgggccgggg
     3721 cgctcggcca gccgcaattc atgccaagct cgtttctcaa agaagcggtc gatttcgacg
     3781 gcgacggcca tcccgatatc tggaattcga cgcccgacgt cctcgcctcg atcgccagct
     3841 atctcgtcga ttacggctgg gtgaaggggc gcgactgggg tttcgaggta acggtgccgg
     3901 ctggcatatc ctgctcgctg gaagggccgg accagggcaa gaggatttcc cagtgggcgc
     3961 aaatgggcat cgagcgcatc ggcggaaagc cgtttccggc ggcggaggcg cgcgcccagg
     4021 gttttctttt gatgccggca ggccgcgaag ggccggcctt catcgtgacg ccgaatttct
     4081 acgtgctcaa ggaatataat atgagcgatc tctatgcgct gttcatcggc catagcgccg
     4141 accgcatcgc gcatggcgac aggcggttta ccgccggttg gggggcggtt ggcgggcttt
     4201 accggtccga cgtggccgcc ctgcaaaggg cgctggagaa gaagggctac gatgtaggct
     4261 cggcagatgg ccttcccggc ttcaagaccc gccgttccat cggtgcatgg caggaaaaga
     4321 acggccgcgc cgccacctgc tttcccgatg cggcaatggt gaaggcgctg aaatagggct
     4381 ttttcgcccg gcatgatccc atccgaaagc cggcttctga ttttcggaca gggatcggca
     4441 ctatgccgcc tgcgacagcg cccggttagc ctcggccaga atctcgaacg aacgcacccg
     4501 cgcgccatga tcgaagatct gcgcggtgac catcagttcg tcggcgccgg tgcggcgcag
     4561 gaatgcctcg acgccctgcc gcaccgtctc cggcgagcca accacggcgc aggacagcgc
     4621 ctgcccaagc atggttttcg ccatcggatc gagatcgcgg tcataatttt ccaccggcgg
     4681 cggcaagcgg ccggcgcggc cggtgcgcaa attgacgaaa gcctgctgca acgaggtgaa
     4741 cagcaactgc gcctcggcgt ctgtcggcgc ggcgaagaca ttgaggccaa gcatcacata
     4801 aggcttggcg agttgttgcg acggctggaa gcgcgagcgg taaatctcga ttgcatggtc
     4861 gagttcggcg ggcgcgaagt gcgaggcaaa cgcgtaaggc aggccgagca cggcggcaag
     4921 ctgagcgcca tagagactgg agcccagtat ccataccggc acttcctggc cctcgccggg
     4981 cacggcgcgg atgcgctggc cttcctcggc aggctcgaaa tagcggatca gttctaccac
     5041 gtcctgcgga aacgtatcga cgccggcctc catgttgcgg cgcagggcgc gggccgtcag
     5101 catgtcggtg cccggtgcgc ggccaaggcc gagatcgatg cggcccggat gcagcgccgc
     5161 cagcgtgccg aactgctcgg caatgaccag cggcgcgtgg ttgggcagca tgatgccgcc
     5221 cgagccgacg cggatgcttt cggtgccgcc ggcaatatgg gcgatgacga cggcggtcgc
     5281 cgcgctggcg atgcccggca tgttgtggtg ctcggccagc cagtagcgct tatagccgag
     5341 ccgctcggcg tggcgggcga gatcaagaga attggcgagc gactgcgacg ggttggagcc
     5401 ttctgcaacg ggcgacaggt cgaggacgga caaggtggcc atggggcata tttccagtga
     5461 ggttcgaagc cgcatatagg gtgcgcccgc ccctgccgca aataacgtgt ccgtcaggcc
     5521 gtcagcctgg cggcctcttc cttgtcctcc tcctcgatga ccagggcctg ctgcgctgac
     5581 aggaaattgc cgacatggcg caagccgtcg aaatggtcgc agaacacctt gaccacgacc
     5641 agcagcggca cagccatcag cgcgccgaca aaaccccaca tccacgacca gaaggcgatg
     5701 gcgataaaaa tggcgacgga gttgatttcc agccgccgcc cgacgatcat cggggttacg
     5761 aactggcctt cgacgatgtt gcacagcaaa acgaaagcgg gcgccagcat ggcgtaggcg
     5821 atgctgtcga agctgatgag cgcgatgacg ccgaccagca cgacattcat cagcgcgccg
     5881 atatagggca ggaaattgag aagtgccgca gttgcgcccc acagcatcgg attaggcatg
     5941 cccagcgccc acaggccaag cgcgacagcc acgcccaggc cggcattgat gagggcgacg
     6001 gtgagcagat agcgcgagat ttcgcgctcg acatcgtaca ccacccgcaa ggcgcgcttc
     6061 ttttcgctca tgcgggtgaa ggactggacg atcttttcat aaaacatcgt gccggatgcg
     6121 agcaggaaca gcgacaaaac gaagacgatg gtgatgctgg tgacgccgga aagcacgctg
     6181 ccggcggcct gcgagacgat gcccgtttgc gagacgacca ccctggaaac gcccggctgc
     6241 tgcgaaggtt cggtcagtgc gccgatctgc tcggtgatct tcatcaattt ctcgaacggg
     6301 cggcgcagat ggtccagccg ctcgctgagt tgctggccga tcgagggagc attattgacc
     6361 aggtcgatag cgggtccgct gagcagatag ccggcggtcg tggcaaatcc gagcgaaagc
     6421 aggaccagca gcgtggccga aatcgcctcg ggcacgccat gcttgcgcaa aagccggaca
     6481 atcggcgtca gcatcagcgc gagaagaaaa gcgagaatga ccggcatgaa aaaatcccgg
     6541 ccgtaataga gcgcaaacac cgcagcgaac aggaacaggc cgacaagcat cgaccgcatg
     6601 agccggatat cggcgcgaac gtcgctggca accggaactt ggccgcttga aggcgtgttg
     6661 tcggtgctca tgattgtccc tcatcgtctg gcattcgcgc caaaatatgc gccttcgaag
     6721 ccataaaccg caacgtcatg ccgccggcat aggttccgcc ttcagggcac aacctgccgt
     6781 gcggaggcgt ttgcggcgtc gattagccat tgcggacaac aaagccgttt gccgcaggcc
     6841 aaaaatcccg ctacaccgcc gccgttcttt catgccagcc gggaaccaag ccgatgacgg
     6901 acgtagctgc caccgccgca atgcaggccg cactgctttc ggcggcgttg ccctacatgc
     6961 aacgctatga aaacaagacg gtcgtggtga aatatggcgg ccacgccatg ggcgatacca
     7021 gccttggcaa ggcattcgcc cgcgatatcg cgct
//
LOCUS       DASYCS010000307         2564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1968105,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000307 DASYCS010000000
VERSION     DASYCS010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1968105"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(218..697)
                     /locus_tag="VGM46_09645"
     CDS             complement(218..697)
                     /locus_tag="VGM46_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HEY4192895.1"
                     /translation="MIRYALACDKGHHFESWFADSAAYDKQAKRKLVSCPVCGSAKVD
                     KAIMAPRVSTSKKRAKAAVEPPAPVAAENTQENSQVAMISPQEQEFRTKLKELREHLT
                     KNSDNVGTKFPEEARKMHYGEIEHRSIYGEASPKDAKELLDEGIEFHPLPVLPDERN"
     gene            complement(694..1569)
                     /locus_tag="VGM46_09650"
     CDS             complement(694..1569)
                     /locus_tag="VGM46_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEY4192896.1"
                     /translation="MTGTSATFKAAMIQMRSGLSPAANFDDASRLIGEAKTAGADYAQ
                     TPEMTNLLAANREQLFSAIADEEHDASLAGYRELARKHGIFIHVGSLAIKVAPDKAAN
                     RSFLITPQGDIAARYDKIHMFDVDLGNGESYRESRNYKPGERAVVSNLPWGVLGMTVC
                     YDLRFPALYRALAEAGATMLAIPSAFTKPTGEAHWHVLMRARAIENGSFVLASAQAGK
                     HESGRETYGHSIIVDPWGRVLAEGGEEPGVIIAEIDPAQVTKARSRVPSLQHGRRFEI
                     AEPMAEAAHLHLVRE"
     gene            complement(1566..1832)
                     /gene="grxC"
                     /locus_tag="VGM46_09655"
     CDS             complement(1566..1832)
                     /gene="grxC"
                     /locus_tag="VGM46_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008598069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEY4192897.1"
                     /translation="MQPVEIYTTGFCPYCVAAKSLLKRKGVDFKEISVSGDNARRQEM
                     IQRANGRMTVPQIFVGETHVGGSDDLHALERAGKLDTLLQGPSA"
     gene            complement(1862..2563)
                     /locus_tag="VGM46_09660"
     CDS             complement(1862..2563)
                     /locus_tag="VGM46_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEY4192898.1"
                     /translation="MPPLCPSCREPLGEGVGLCAGCWSKLSFIERPYCARLGIPFTYD
                     PGPGLLSMEAIAAPPAYDRARAAVRYDEIARALVIAFKFSDRLDLAPMMGRWMARAGR
                     ELTEGADALVPVPLHWRRLWARRFNQAAALSGIISGIAGVPVLHGVLNRVRATPQQVG
                     LSKPERADNVQGAFRVPPEAKARIAGKRLILVDDVLTSGATVDTCARALLRAGAAHVD
                     VLVFARVVAPLRNTI"
BASE COUNT          447 a          795 c          846 g          476 t
ORIGIN      
        1 caagatagaa actgcccgtg acactgtggc cgcaaccgga tgtttgccgg aacgaactac
       61 aggggaacgc gccatgttga ccgtcgaaca cttcactgcc gaagcgctgc gtctgcaacg
      121 tctcagggcc tatgcggtcc accatggcca tcaggaccgc ctgcgctggc ttttcgaaat
      181 gtttgggttt cgcgcgtcga agcggactta aggccgctta attccgttcg tccggcaaaa
      241 ccggcaacgg gtggaattcg atgccctcgt cgagcaactc cttggcgtct ttcggcgaag
      301 cttcgccgta gatcgagcga tgctcgatct cgccgtagtg catcttgcgt gcttcctcgg
      361 gaaacttggt accgacattg tcggaattct tcgtcaggtg ctcgcgcagt tccttgagct
      421 tggtgcggaa ttcctgctcc tgaggcgaga tcattgccac ttgtgagttt tcttgcgtgt
      481 tctcggccgc aacaggcgcg ggcggttcga cggccgcctt ggcgcgcttc ttggacgtcg
      541 agacgcgcgg cgccatgatg gccttgtcga ccttggccga accgcacacg gggcacgaca
      601 cgagcttgcg cttggcctgc ttgtcgtagg ccgcggagtc ggcaaaccag ctttcgaagt
      661 gatggccttt gtcgcaggca agcgcgtagc ggatcattcg cgcaccagat gcaaatgtgc
      721 ggcctcggcc atcggttcgg cgatctcgaa acggcggccg tgctgcagcg aaggtacgcg
      781 cgaacgcgcc ttggtcactt gcgccggatc gatttcggcg atgatgacgc cgggttcctc
      841 gccaccttcg gccaggacgc ggccccacgg atcgacgatg atcgaatggc cgtaagtttc
      901 acggccgctt tcatgtttcc cagcttgcgc cgatgccagc acgaagctgc cgttttcaat
      961 ggcacgggcg cgcatcagca cgtgccagtg cgcttcaccg gtcggcttgg tgaaggcgga
     1021 gggaatggcg agcatcgtgg cgccggcttc ggccagcgcg cgatagagcg ccgggaagcg
     1081 cagatcgtaa caaaccgtca tgcccagcac gccccaaggc agattactaa cgacggctcg
     1141 ttcgccgggc ttgtagttgc gcgactcgcg gtaactctcg ccattgccga ggtcgacgtc
     1201 gaacatatgg atcttgtcgt aacgcgcggc gatgtcgcct tgcggcgtga tcaggaagga
     1261 gcggttggcg gccttgtcgg gcgcgacctt gatggccagc gagccgacgt gaatgaatat
     1321 gccgtgctta cgcgccaact cgcgataacc ggcgagcgac gcgtcgtgct cctcgtcggc
     1381 gatcgcggag aaaagctgtt cacggttggc cgccagcaga ttggtcatct ccggtgtctg
     1441 cgcgtagtcg gcgccggcag tctttgcttc gccgatcaag cgtgaggcgt catcgaaatt
     1501 ggcggcgggc gacaggcccg accgcatctg gatcatcgcg gccttgaagg tcgcggacgt
     1561 accggtcatg ccgaaggtcc ttgcaggagt gtgtccagct ttccggcgcg ttccagcgcg
     1621 tgcagatcgt cgctgccgcc gacatgcgtt tcgccgacga aaatctgtgg caccgtcatg
     1681 cggccattgg cccgctggat catctcctgg cgcctggcgt tgtcgccgct gaccgaaatt
     1741 tccttgaagt cgacgccctt gcgcttgagg agcgatttgg cggcaacgca gtaggggcaa
     1801 aagccggtgg tgtagatttc gacgggctgc atatgggatc cagtgaatgt attacgccac
     1861 tttatatggt gttgcgcagt ggcgcgacaa cccgggcgaa aaccagcacg tcaacatggg
     1921 cggcgccggc ccgcagcaac gcccgggcgc aggtgtcgac cgtggcgccc gaggtcagca
     1981 cgtcgtcgac caatatcagt cgcttgcccg cgatccgggc cttggcctcc ggcggcaccc
     2041 ggaaggcgcc ctgcacattg tcggcgcgct cgggcttgga gaggcccacc tgctgcggcg
     2101 tagcccggac gcggttgagg acgccatgca ggaccggcac tccggcaatg ccggaaataa
     2161 tgccggacag cgccgcggcc tggttgaaac gccgggccca gagcctgcgc caatgtagcg
     2221 gcacaggcac cagtgcgtcg gcgccctcag tcagttcgcg gccggcccgc gccatccagc
     2281 ggcccatcat cggcgccaga tcgagccgat cgctgaactt gaaggcgatc accagcgcgc
     2341 gggcaatctc gtcgtagcgc accgcggcac gcgcccggtc ataggccggc ggggcggcga
     2401 tggcttccat agacagcagg cccggacccg ggtcataggt gaagggaatg ccgagccgcg
     2461 cgcaatacgg cctctcgatg aaggagagtt tcgaccagca gccggcgcac aggccgacgc
     2521 cctcgcccag cggttcgcgg caggacgggc agagcggggg caac
//
LOCUS       DASYCS010000308         3743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1824195,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000308 DASYCS010000000
VERSION     DASYCS010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3743
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1824195"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..1707)
                     /locus_tag="VGM46_09665"
     CDS             complement(61..1707)
                     /locus_tag="VGM46_09665"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEY4192899.1"
                     /translation="MAPAPLDLDPELLAAAAESKAWPFEEARKIVARYAKTGYPEAVL
                     FETGYGPSGLPHIGTFGEVARTTMVRHAFRILTQDKVKTRLLCFSDDLDGMRKIPENV
                     PDRAALEPYLHMPLTSVPNPFGGDYDSFGHHNNAMLRRFLDTFGFDYEFASATEYYRS
                     GRFDAVLLRAAERYDDIMAVMLPTLGPERQATYSPFLPISPKSGRVLYVPMKHVDAKA
                     GTVTFDDEGTETTLPVTGGKVKLQWKPDFGMRWAALGVDFEMFGKDHQTNAAIYDKIC
                     VILGGRAPDHFVYELFLDDKGQKISKSKGNGLTIDEWLTYAPTESLGLYMYQKPRVAK
                     KLYFDVIPRAVDEYYTFLSAYARQDWKNRLGNPVWHMHGGNPPAIDLAVPFSLLLNLV
                     SASNAQDKAVLWGFISRYAPGVTPASNPELDRLVGYAIRYFDDFVKPAKVFRAPDDVE
                     RAALTELAAVLAALPAGADGETIQNAALNVARKIERYQDHSKQAPEGGPGVSVAFFQM
                     IYQVLIGQERGPRFGSFAALYGTAETCALIERALAGKLTA"
     gene            1847..2260
                     /locus_tag="VGM46_09670"
     CDS             1847..2260
                     /locus_tag="VGM46_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HEY4192900.1"
                     /translation="MPLPTVHEALIYLMVVASASDRDMSDAELARIGEVVRAWPVFED
                     FDSRRLVGVAQDCQIMLHEGNGLDDVLDLAKRVIPARLHDTAYAAVFEVVVSDLEMRM
                     EEVRVLQRLREALELDPLAVAAIELANKARHRTLT"
     gene            complement(2272..3075)
                     /locus_tag="VGM46_09675"
     CDS             complement(2272..3075)
                     /locus_tag="VGM46_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY4192901.1"
                     /translation="MPAPVLRKAPVFRDAPILWDAKERLPKPDLSGLARLRFLTTTDF
                     APLNFLDRDGRLSGFHIDLARAICAKLEIVEKCQIQALPWPELDGALQRDEGEAIIAG
                     IAVTPESRAKYAFSRAYLRFPARFIMARQSLVGEPLYENLKTRRVGVIAGSAHERMLR
                     DYFGGVEIVALAGPEELYAAVKTGAVDAGFGDGMRLAIWLAGPQAAGCCRFAGGPYLA
                     PEYLGEGLAIAAKADNPALAAAFDYALQEISRDGTFAELYLRYFPVGFY"
     gene            3478..>3743
                     /locus_tag="VGM46_09680"
     CDS             3478..>3743
                     /locus_tag="VGM46_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192902.1"
                     /translation="MRPFWFEDGGNASSTDCEQAKAQGEAPEDASYRAHRRPGAHLAE
                     IFFPELTEWQSVLARLSVPLQTAVEIAVRARSDASDFQSAMLACG"
BASE COUNT          693 a         1129 c         1227 g          694 t
ORIGIN      
        1 attctggcgc gcctgcttct ggctcgccta attctggctc gattgattcg agcggcgtgg
       61 ttacgccgtc agtttcccag ccagcgcccg ctcaatgagc gcgcaggtct ccgccgtgcc
      121 ataaagcgcg gcgaacgaac cgaagcgcgg tccacgttcc tggccgatca gcacctggta
      181 gatcatctgg aagaaggcga ccgagacgcc cggtccgcct tccggcgctt gcttggaatg
      241 gtcctggtag cgttcgatct tgcgcgcgac gttgagcgcg gcgttctgga tcgtctcgcc
      301 gtcggctccg gcgggcaggg cggccagcac cgccgccagt tcggtgagcg ctgcacgctc
      361 gacatcgtct ggagcgcgaa aaacctttgc cggcttcacg aaatcgtcga aatagcggat
      421 ggcgtagccg accagccggt cgagttccgg gttgctggcc ggcgtgacgc ccggcgcata
      481 gcgcgagatg aatccccaca gcacggcctt gtcctgcgcg ttcgacgcgc tgaccagatt
      541 gagcagcagc gagaacggta ccgcaagatc gatggccggc ggattgccgc catgcatgtg
      601 ccagaccgga ttgccgagcc ggttcttcca gtcctgccgc gcgtaggcgg acaagaaggt
      661 gtaatattca tcgacggcgc gcggaatgac atcgaagtaa agcttcttgg ccacgcgcgg
      721 cttctggtac atgtaaaggc caaggctttc ggtcggcgca taggttagcc attcgtcgat
      781 ggtcagcccg ttgcccttgg acttggaaat cttctggccc ttgtcgtcta ggaacagttc
      841 gtagacgaaa tgatccggcg cgcgcccgcc caggatcacg caaatcttgt cgtagatggc
      901 cgcattggtc tggtggtcct tgccgaacat ctcgaaatcg acgcccagcg ccgcccagcg
      961 catgccgaaa tcgggcttcc attgcagctt taccttgccg ccggtgacgg gcagcgtcgt
     1021 ctcggtgcct tcgtcgtcga aggtgacggt gccggccttg gcgtcgacat gcttcatcgg
     1081 cacgtagagc actcgcccgc ttttgggcga gatcggcagg aacgggctat aggtcgcctg
     1141 ccgttccggc cccagcgtcg gcagcatcac cgccatgatg tcatcatagc gctcggcggc
     1201 gcgcaaaagc accgcgtcga agcggccgga ccggtaatat tccgtggcgc tggcgaattc
     1261 atagtcgaag ccgaacgtgt cgaggaagcg gcgcagcatc gcgttgttgt gatggccgaa
     1321 actgtcatag tcgccgccga acggattggg caccgaagtc agcggcatgt gcagataggg
     1381 ttccagcgcc gcacggtccg gcacgttttc gggtatcttg cgcatgccgt cgaggtcgtc
     1441 ggagaagcac aaaaggcgcg tcttgacctt gtcttgcgtc aggatgcgga aggcgtgccg
     1501 caccatggtg gtgcgcgcca cttcgccgaa cgtgccgata tgcggcaggc cggatgggcc
     1561 gtagcctgtc tcgaacagca ccgcctccgg gtagcccgtc ttggcgtaac gcgcgacgat
     1621 cttgcgggct tcctcgaacg gccaggcttt gctttccgcc gcggcggcga gaagttcggg
     1681 gtcgagatcg aggggcgcgg gtgccatggc aactatcctg tctttgcgcc atgctctagg
     1741 cgggcggcgc caaagcgtca acgcggcgcg ccgtttttcc ttgcggcggc gccaaacctg
     1801 cctacctgtg ggcgagccat ttggcgacgt gatggagaag acgaacatgc ccttgccgac
     1861 cgtacatgaa gcgctgatct acctgatggt ggtggcgtcg gcctccgacc gcgacatgtc
     1921 ggatgcggag cttgcccgca tcggcgaagt cgttcgcgcc tggcccgtct tcgaggattt
     1981 cgacagccgc cggctcgtcg gcgtggcgca ggattgccag atcatgctgc atgaaggcaa
     2041 cggccttgac gatgttctcg atctggcaaa gcgggtgatc ccggcgcggc tgcacgacac
     2101 ggcttatgcg gcggtgttcg aggttgtcgt gtccgatctc gaaatgcgga tggaggaggt
     2161 ccgcgtcctg caaagactgc gcgaggcgct ggaactcgac ccattggcag tcgccgccat
     2221 cgaactggcc aacaaggcgc gccatcgcac gctgacctga gcggtctgtt tctagtaaaa
     2281 tccgaccgga aaatagcgta ggtaaagctc agcgaacgtg ccgtcccgcg agatctcctg
     2341 caacgcatag tcgaaggcgg cggcgagggc cggattgtcg gccttggccg cgatggccaa
     2401 gccttcgccc agatattccg gcgccagata agggccaccg gcgaaccggc agcatccggc
     2461 tgcttgcggc ccggccagcc agatggcgag ccgcatgccg tcgccgaaac cggcatcgac
     2521 ggcgccggtt ttgaccgccg cgtaaagctc ctccggcccg gcaagggcga cgatctcgac
     2581 gccgccgaaa taatcccgaa gcatgcgctc atgggcggag ccggcgatga cgccgacacg
     2641 cctggtcttg agattttcat aaagaggttc gccgacaagg ctttgcctgg ccatgataaa
     2701 gcgcgccgga aagcgcagat aggctcgcga aaaagcatat ttcgcccgag actccggcgt
     2761 gaccgcgatg ccggcgatga tcgcctcgcc ctcgtcccgt tgcagggcgc cgtccagttc
     2821 cggccacggc agcgcctgta tctggcattt ttccacaatt tcgagcttgg cgcaaatggc
     2881 gcgggccaga tcgatatgga agccggaaag ccggccatcg cggtccagga aattgagcgg
     2941 cgcaaagtcg gttgtcgtca aaaaccgcaa tcgcgccagg cccgaaaggt cgggttttgg
     3001 cagacgctcc ttcgcgtccc agaggatcgg ggcgtcccga aaaaccgggg ccttccgaag
     3061 aaccggagcc ggcatttcgg ccgcctgcga aaccgcgccc aacggcaacg cgccggccgc
     3121 cgtcatcaat gcaaggcata gccatcggac cgcgcagaac cgaaccgcgg agtgccggaa
     3181 ttgcaagctc gccaacgccg caatgatttt ttccatatag gcatttggta tgaaatgggg
     3241 gccggcacaa cgcttttcca tttcaatgca tcgaattggc gatatggtca tccgtgtcgg
     3301 ggattggatg gatgacgggt gggcaggggg aggccactgt cttgatcgcc tcggcaacat
     3361 gagggggttg caaatggcgc ggtctgccgg cgaagcggtt atgacgcgac ggtgacggcc
     3421 gcaatattgg aagaaaggcg caagggcttc cgcctttcga tcgggggata aaaggggatg
     3481 cggccttttt ggttcgagga tggcggcaac gcctcgtcga cggattgcga gcaagcgaag
     3541 gcgcaagggg aagcgccaga ggatgcttcc tacagggcgc accggcggcc aggcgcgcat
     3601 ctggccgaaa tcttctttcc ggaactgact gagtggcaat cggtcctggc aaggctttcc
     3661 gtgccgctcc agaccgccgt ggagattgcc gtgcgcgcgc gaagcgacgc cagcgatttt
     3721 caatccgcca tgctggcttg cgg
//
LOCUS       DASYCS010000309         5515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4141272,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000309 DASYCS010000000
VERSION     DASYCS010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 5515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5515
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4141272"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VGM46_09685"
     CDS             complement(<1..266)
                     /locus_tag="VGM46_09685"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEY4192903.1"
                     /translation="MWTGVIPAVTTKFTEDDRLDHAEMERCFALQMDAGCDGLIVAGS
                     LGEGPMLSHDEKIDVLKTARKAAGKKPVLLTVNEAGTREAAVLA"
     gene            367..1026
                     /locus_tag="VGM46_09690"
     CDS             367..1026
                     /locus_tag="VGM46_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006327590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY4192904.1"
                     /translation="MALTDENSAFEPAATRAYRALERMIIMLELVPGSFVTEGALIEK
                     LGLGRTPVREAIQRLAWESLLDIRPRAGIAVAPLHAGDWVRVLDARRGVEIVLARSAA
                     RYVTRAAADQFHGAALAMQAAAVSGSVQAFLEADKAFDEALAFAADNVFAARLAAPLQ
                     THSRRFWFRYKAETGLAEAAEHHVGLIRSILSGDEEIAAKDCERLMLLLRSHAETAAR
                     R"
     gene            complement(1181..2674)
                     /locus_tag="VGM46_09695"
     CDS             complement(1181..2674)
                     /locus_tag="VGM46_09695"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY4192905.1"
                     /translation="MIHYGHYIGGKNVKGTSGREADVFQPLDGTVRAKVALASRAELD
                     AAVANAKAAQPAWAATNPQRRARVFMKFLELVARDNDALAELLAREHGKTIPDAKGDI
                     LRGVEVVEFALGVPHLMKGEYTEGAGPGIDLYSMRQPLGVVAGITPFNFPAMIPMWKF
                     APAIACGNAFILKPSERDPGVPMKLAELMTEAGLPDGILNVVNGDKESVDGILDHPDI
                     SAIGFVGSSSIAEYIYSRGSANGKRVQCFGGAKNHMVIMPDADMDQAVDALIGAGYGA
                     AGERCMAVSVAVPVGKATADRLMEKLIPRVESLKIGPSTDPSADFGPLVTQQALDRVK
                     NYVEIGIKEGATLAVDGRGFKMQGYENGFYMGGCLFDNVTADMRIYKEEIFGPVLSVV
                     RAHDFEEAVRLPSEHEYGNGVAIFTRDGDAARDFAARVQVGMVGINVPIPVPVSYYTF
                     GGWKRSGFGDLNQYGPDAFRFYTKTKTVTSRWPSGVKEGAEFAMPTS"
     gene            2840..3721
                     /locus_tag="VGM46_09700"
     CDS             2840..3721
                     /locus_tag="VGM46_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY4192906.1"
                     /translation="MNWDDVRIFLAVARAGQFLGAARRLELNHATVSRRIAALEEALR
                     SKLFRRLTTGSELTPAGVRFLDIAERMEAEMITARSTLAGEGDDISGTVRIGAPDGFG
                     VAFLARHLGALTAAHRELTIQLVPVPRSFSLSRREADIAITVERPTEGRLVAGKLVDY
                     TLGLFASRAYAKAHGLPKSPADLASHTLVGYVPDLIVSPSLDYDAEFSPDWRSAFAIS
                     SALGQAEAVRAGAGIGILHTFIARSMPELIPVDIVAPIRRAYWLVYHESVRPMRRVQL
                     VAGYITKAVERERALFI"
     gene            complement(3718..5022)
                     /locus_tag="VGM46_09705"
     CDS             complement(3718..5022)
                     /locus_tag="VGM46_09705"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HEY4192907.1"
                     /translation="MDHSAARQDRAGQGERAMKILVLGGGVVGVTTAYYLTEAGHEVT
                     VIDRQQGPALETSFANAGEVSPGYSSPWAGPGVPVKAVKWLLMKYGPLVIRPVMDPHM
                     WSWVARMLRNCTAERYALNKSRMVPIAEYSRDVLKALRASTNIAYDDRAKGTLQLFRK
                     QYQLDGIGGDVEVLKQYGVPFNVLDQDGCIAAEPGLAGVREKFVGGLQLPGDETGDCQ
                     IFTTRLAEMAAAAGVKFEFGVTLNAIHADKGQITGCSTSAGERKADAYVMAMGSYSPI
                     WMRKLGQSIPVYPIKGYSITVPIKNAAIAPESTVMDETYKIAITRLGNRIRVGGTAAI
                     AGYDLSLHEGRREALAHSVGDLFPGAGDLGAASFWTGLRPMTPDGPPIIGASKYPNLY
                     LNTGHGTLGWTMACGSARVLSDIVSGRKPEIDASKLGPERYA"
     gene            complement(5068..>5515)
                     /locus_tag="VGM46_09710"
     CDS             complement(5068..>5515)
                     /locus_tag="VGM46_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine racemase C-terminal domain-containing
                     protein"
                     /protein_id="HEY4192908.1"
                     /translation="RPGAALYGINPVPDLANPMLAVVRLEARVIQTRTLTAGAGIGYG
                     HTVVTDGPRTVATISLGYADGWRRRAVAAAHVDAVSLPFIGRVSMDSIVLDISALPEG
                     RLKAGDHVQLIGPSQTLDEVAGFTGTIAYEVLTSLGHRFHRQYLGA"
BASE COUNT          960 a         1881 c         1683 g          991 t
ORIGIN      
        1 ttggcaagca cggccgcctc gcgggtgccg gcctcgttga cggtcagcag caccggcttc
       61 ttgccggccg ccttgcgcgc cgtcttcagc acgtcgatct tctcgtcatg cgaaagcatc
      121 gggccttcgc ccagcgaacc ggcgacgatc aggccgtcgc atccggcgtc catttgcagg
      181 gcgaagcaac gctccatctc ggcatggtcg aggcggtcat cctcggtaaa cttggtcgta
      241 acggcgggga taactccggt ccacatagct ctgctccatc tgatatatca accgtatatc
      301 aatcttgacg cccactgtca atacggttcc agctgtgata cgcgggaaat atatcaggag
      361 gcattcgtgg cacttaccga cgaaaactca gcctttgaac ccgctgcgac gcgggcctac
      421 cgggcgctcg agcggatgat tatcatgctc gaactcgttc cgggaagctt cgtcaccgaa
      481 ggagcgctga tcgaaaagct cggccttggc cgcacgcccg tgcgtgaggc gatccagcgg
      541 ctggcgtggg aaagcctgct cgacatccgc ccgcgcgccg gcatcgccgt cgccccgcta
      601 cacgccggcg actgggtgcg ggtgctggac gcccgccgcg gcgtcgagat cgtgctggcc
      661 cgctcggcgg cgcgctacgt cacccgcgcg gctgccgacc agtttcatgg cgcagccctt
      721 gccatgcagg ccgccgccgt ctcgggcagc gtgcaggcct ttctcgaagc cgataaggcc
      781 ttcgacgaag cgctggcctt tgccgccgac aatgtttttg ccgcgcgtct tgccgccccc
      841 ctgcaaaccc acagccgccg tttctggttt cgctacaagg ccgaaaccgg cctggccgaa
      901 gccgccgaac accatgtcgg gttgatccgc tcgatcctca gcggcgacga ggaaattgcg
      961 gcaaaggatt gcgaacgcct gatgctgctc ttgcgcagcc atgccgagac ggcggcacgg
     1021 cgctagagcg gttccgattt cgctctatct gtttgttttg gccgcatttc cggacgcaaa
     1081 accgtttcac acttttgctg gaaatgctct aaattctgtc gcgcaaaaag aaaaagggcg
     1141 ccgcaaccgc cgcgcccctc cttttcgctc gataaccgcc tcaggaagtc ggcatcgcga
     1201 attccgcgcc ctccttcacg ccggaaggcc agcgcgaggt aacggtcttg gtcttggtgt
     1261 agaagcggaa cgcatccggg ccatactggt tgagatcgcc gaagccggaa cgcttccagc
     1321 cgccgaaggt gtaatacgac accggcaccg ggatcggcac gttgatgccg accatgccga
     1381 cctggacgcg ggcggcgaag tcgcgggccg catcgccgtc gcgcgtgaag atggcgacgc
     1441 cattgccata ttcatgctcg gaaggcagcc gcaccgcttc ctcgaaatca tgcgcgcgca
     1501 ccaccgaaag caccggcccg aagatttctt ccttgtagat gcgcatgtcc gccgtcacat
     1561 tgtcgaacag gcagccgccc atgtagaagc cgttctcgta gccctgcatc ttgaagccgc
     1621 gcccatcgac cgccagcgtc gcgccttcct tgatgccgat ctcgacataa ttcttcacgc
     1681 ggtcgagcgc ctgctgggtg accagcgggc cgaaatcggc cgacggatcc gtcgaagggc
     1741 cgatcttcag gctttcgacg cgcggaatga gcttttccat cagcctgtcg gcggtagcct
     1801 tgcccaccgg cacggcgacg gaaaccgcca tgcagcgttc gccagccgcg ccgtagcccg
     1861 cgccgatcag cgcatccacc gcctggtcca tgtcggcatc cggcatgatg accatgtggt
     1921 tcttcgcgcc gccgaagcac tgcacgcgct tgccgttggc ggaaccgcgc gaataaatat
     1981 attcggcgat cgacgacgaa ccgacgaagc cgatggccga aatgtcggga tggtcgagga
     2041 tgccgtccac cgattccttg tcgccattga ccacattgag gatgccgtcc ggcaggccgg
     2101 cctcggtcat cagttcggcc agtttcatcg gcacgcccgg atcgcgctcg gaaggcttga
     2161 ggataaaggc attgccgcag gcgatggccg gggcgaattt ccacatcggg atcatcgccg
     2221 gaaaattgaa cggcgtgatg ccggcaacga cgccgagcgg ctggcgcatc gaatagaggt
     2281 cgatgccggg gccggcgcct tccgtgtact cgcccttcat caggtgcggc acgccgagcg
     2341 cgaactcgac gacctcgacg ccgcgcagga tgtcgccctt ggcgtcggga atggtcttgc
     2401 catgctcgcg ggcgagcagt tcagccagcg catcgttgtc acgcgccacc agttcgagga
     2461 atttcatgaa cacgcgcgcc cgccgctgcg ggttggtcgc cgcccatgcg ggctgcgccg
     2521 ccttggcgtt ggcgacggcg gcgtcaagct ccgccctgga agccagcgcc accttggcgc
     2581 gcacggtgcc gtccagcggc tggaacacat cggcttcacg gccggaagtg cccttgacgt
     2641 tcttgccgcc gatataatga ccgtaatgga tcatgctttc tctccctgcg atcggtgatg
     2701 atgcctcact gtcggccttt gcccgaccaa tggcaacgcg aactgagacg caaccgttgt
     2761 gcgaaatttg atatagcttc caagaaggaa tatgctttca cgcaaattca ggggaaagtc
     2821 tgccatatga accggccaca tgaactggga tgacgtgcgc atatttctcg cggtcgcccg
     2881 cgccggccag ttccttggcg cggccaggcg gctggaactg aaccatgcca ccgtttcgcg
     2941 ccgcatcgca gcacttgagg aggccttgcg gtcaaaactg tttcgccgcc tcaccaccgg
     3001 cagcgaattg acgccggcgg gcgtgcgctt cctcgacatt gccgagcgca tggaggccga
     3061 gatgatcacc gcccgctcga cgctggcagg cgagggcgac gacatttccg gcacggtgcg
     3121 catcggcgcg cccgacggct tcggcgtcgc cttcctggca aggcatctcg gcgcgctgac
     3181 ggcggcgcac cgcgagctga ccatccagct cgtgccggtg ccgcgctctt tctcgctttc
     3241 ccggcgcgaa gccgacatcg ccattacggt cgagcgcccg accgaaggcc ggctggtggc
     3301 gggcaagctg gtcgactaca cgctcggcct gtttgcctcg cgcgcttatg ccaaggccca
     3361 tggcttgcca aaatcgcctg ccgatctcgc cagccacacg ctggtcggct atgtgcccga
     3421 cctgatcgtc agcccctcgc tcgactatga tgccgagttc agcccggact ggcgcagcgc
     3481 cttcgccatc tcgtcggcgc tgggccaggc cgaagcggtg cgcgccggcg ccggcatcgg
     3541 catcctacac accttcatcg cccgctcgat gccggaactg atcccggtcg atatcgtcgc
     3601 gcccatccgc cgcgcttact ggctcgtcta tcacgaatcg gtacggccca tgcgccgcgt
     3661 ccagctggtc gccggctaca taaccaaggc ggtggaacgc gaacgggcgc tgtttatcta
     3721 ggcgtagcgc tctggcccaa gcttgctggc gtcgatctcc ggcttgcggc ccgagacgat
     3781 atcggagagc acccgcgccg aaccgcaggc catcgtccag ccaagggtgc cgtggccggt
     3841 attgaggtag aggttcggat atttcgatgc cccgatgatc ggcgggccgt ccggcgtcat
     3901 tggccgaagg cccgtccaga agcttgccgc gcccaggtcg cccgcgccgg gaaacaggtc
     3961 gcccaccgaa tgcgccagcg cctcgcgccg tccttcatgc agggaaaggt catagccggc
     4021 gatagccgcc gtgccgccga cccggatacg gttgccaagc cgggtgatgg cgatcttgta
     4081 ggtctcgtcc atcaccgtcg attcgggcgc gatggcggcg ttcttgatcg gcacggtgat
     4141 cgaataaccc ttgatcggat agaccgggat cgactggcca agcttgcgca tccagatcgg
     4201 cgaatagctg cccatcgcca tcacataggc gtccgccttc cgctcgcccg cactggtcga
     4261 acagccggtg atctggccct tgtcggcgtg gatggcgttc agcgtcacgc cgaactcgaa
     4321 cttcacgccc gccgccgccg ccatctctgc cagccgcgtg gtgaatatct ggcagtcgcc
     4381 ggtctcgtcg cccggcaatt gcaggccgcc gacaaacttc tcgcgcacgc cggccagccc
     4441 cggctcggcg gcgatgcagc catcctggtc gagcacgttg aacggcacgc catactgctt
     4501 gagcacctcg acgtcgccgc cgatgccatc gagctgatac tgcttgcgaa acagctgcaa
     4561 cgtgcctttg gcgcggtcgt cataggcgat gttggtcgag gcccgcagcg ccttcagaac
     4621 atcgcggcta tactcggcaa tcggcaccat gcgcgacttg ttcagcgcat aacgctcggc
     4681 cgtgcagttg cgcagcatcc tggcgaccca gctccacata tgcgggtcca tgacgggacg
     4741 gatcaccagc ggaccgtatt tcatcaaaag ccatttgacc gccttgaccg gcacgcccgg
     4801 cccagcccat ggcgaggaat agccgggcga aacctcgccc gcattggcga agctggtttc
     4861 cagcgccggc ccctgctggc ggtcgatcac cgtcacctca tggccggcct cggtcaggta
     4921 ataggccgtg gtaaccccga ccacgccgcc gcccagcacc aaaatcttca ttgcccgttc
     4981 tccctgcccg gcccgatcct gccgggcggc actatggtcc agccacgcgg gctgctttgt
     5041 tgcgaagtcc gtatgcggcc gccgccctca ggctcccagg tattgcctat ggaagcggtg
     5101 accgaggctg gtcagaacct cataggcaat cgttcccgta aagcccgcaa cctcgtccag
     5161 cgtctgcgaa ggaccgatca actggacatg atcgcccgcc ttcagccgcc cttccggaag
     5221 cgccgaaatg tcgaggacga tcgaatccat cgaaacgcgc ccgataaacg gcaggctgac
     5281 ggcatcgaca tgcgccgcgg cgaccgcgcg ccgcctccag ccatcggcat agcccagcga
     5341 aatcgtcgcc accgtgcgcg gcccatcggt gacgacggtg tggccgtagc cgatgccggc
     5401 gccggcggtg agcgtccgcg tctggatcac cctcgcctcc agcctgacga ccgccagcat
     5461 cggattggca aggtcgggca ccgggttgat gccatagagc gcggctcccg ggcgg
//
LOCUS       DASYCS010000310         4025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3708864,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000310 DASYCS010000000
VERSION     DASYCS010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3708864"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="VGM46_09715"
     CDS             complement(<1..749)
                     /locus_tag="VGM46_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY4192909.1"
                     /translation="MPKLNLPTMNRRAFIVGTAAVGGGLALGLDIPFGGPSIARAADG
                     SPEVNAWVVIQPNDTVVIRIARSEMGQGTLTGLAQLVAEELECDWSKVTTEFPSPGQN
                     VARKRVWGDFSTGGSRGIRTSQDYVRKGGAAARVMLIEAAANEWKVPASECIAANSVI
                     THTPSGKTTTFGKVAEAAAKLTPPADVKLKDPKDWKLAGKGLKRLDTPDKVVGKTVYG
                     IDVKLPGMLNAAIKDCPVTGGKLKSYDEAKIA"
     gene            complement(769..1218)
                     /locus_tag="VGM46_09720"
     CDS             complement(769..1218)
                     /locus_tag="VGM46_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY4192910.1"
                     /translation="MAYLTINGKTVDIDVEADTPLLWAIRENVGLTGTKYGCGIAQCG
                     ACTVHVDGVAMRSCGVLVSEAIGKQITTIEGLATDGALHKVQQAWINNDVPQCGYCQS
                     GMIMAVAALLKDNPKPTDQDIDDAITNICRCGTFQQVREAIHAAAHA"
     gene            complement(1376..2764)
                     /locus_tag="VGM46_09725"
     CDS             complement(1376..2764)
                     /locus_tag="VGM46_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4192911.1"
                     /translation="MDTDITTAKPPSGPVMDGLPWERRRWAVAAIFTALTMASLDTVI
                     ANIALPAIAVDLHVSPADVVWVVNVYQIALVATLLPLGALGEIVGHERIYLGGLLLFT
                     LASLGCACAWSLDSLLVARTLQGLGASGLMTVNTALVRFIYPSRMLGRGFGHNALVVA
                     TAFTLGPTIASGILAIGHWPWLFAINLPFGVIAMLIGWKTLPATPRAVHNFDFFGSVM
                     AAACLGLFIIGIGSAAHKVPPMLVIAELIAALVLGWMLTRRHADHPAPMLPIDLFRRP
                     VFALSALTAVCSFAVQGLSFVALPFYFEDVLQRSQVETGFFMTPWPLVVAIMAPIGGR
                     LSDRYPAGILGGIGLALLAVGMALLAMLPASPSVANIVWRMGVCGLGFGFFQTPNMRA
                     LMSSAPPGRSGSASGIVATARLTGQTTGAALAALCFSLAGHNGAALGLALGAGFAALG
                     SMVSFLRMTVTR"
     gene            3001..>4025
                     /locus_tag="VGM46_09730"
     CDS             3001..>4025
                     /locus_tag="VGM46_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY4192912.1"
                     /translation="MSGQEGLGTELLRGGVTPIGAADGVVIRSGNESNRRRLWTALWV
                     ALLLAILGFLVIYPVLTLLLGALTDTNPVVEGLSLSHLSIANFIAVLINPNVAEALFN
                     TLVVCGGGTLIAVVIGLAFSWIVVRTNTPFKGFIAATSVLPLFAPPLVAGVAWTILGS
                     PKTGLINTMFKWMGLDWHVNFYSLPGLVFVFGIYYAPYVYMFTASALRNMDPSLEEAA
                     EISGASAFATLFSVTFPLIMPAIISGMLLSFIVMLGIYGIPAVLGAPANINVLTTYIF
                     KLTNWSPPLYNTAAAVAILLMMVTGFLVFLQQRVLSGRSYTTVAGKAFRPRNLNLGPW
                     RWFTLGLA"
BASE COUNT          746 a         1245 c         1258 g          776 t
ORIGIN      
        1 gcaatcttgg cttcgtcgta gcttttcagc ttgccgccgg tgaccgggca gtccttgatt
       61 gcagcattga gcatgcccgg cagtttgacg tcgatgccgt agacggtctt gccgaccacc
      121 ttgtcgggcg tgtcgaggcg cttcagcccc ttgccggcga gcttccagtc cttggggtcc
      181 ttcagcttga cgtcggccgg cggcgtgagt tttgcagcag cctcggcgac tttgccgaaa
      241 gtcgtggttt tgcccgacgg cgtatgcgtg atgacgctgt tggcggcgat gcattcggag
      301 gcaggcacct tccactcgtt ggccgcagct tcgatcagca tcacgcgcgc ggcggcgccg
      361 cccttgcgca cataatcctg cgaggtccgg atgccacggc tgccgccggt cgagaagtcc
      421 ccccacacgc gcttgcgggc gacgttctgt ccgggcgaag ggaactcggt cgtgaccttc
      481 gaccagtcgc attcgagttc ttccgcaacg agttgggcaa gcccggtgag cgtaccttgg
      541 cccatttccg atcgcgcgat gcgaatcacg acggtgtcgt tcggctggat caccacccag
      601 gcgttgactt ccggcgagcc gtcggccgcg cgtgcgatcg aggggccgcc aaagggaatg
      661 tcgaggccga gcgcgagacc gccgccaacc gcggcagtgc cgacgataaa ggcgcggcga
      721 ttcatggttg gcaagttcag ttttggcata tgattcatgg ctgccccctc aagcgtgcgc
      781 ggcggcatga atcgcctcgc ggacctgctg gaaggtcccg caacggcaga tattggtgat
      841 ggcgtcgtcg atatcctggt cggtcggctt tggattgtcc ttgagcagcg ccgctaccgc
      901 catgatcatt ccgctttggc aatagccgca ttgcggaacg tcgttgttga tccaggcctg
      961 ctgcaccttg tgcagcgcgc catctgtcgc caacccttcg atcgtcgtga tctgcttgcc
     1021 gatcgcctcg ctgaccaata cgccgcaaga acgcatcgca acgccgtcga catgaacggt
     1081 gcaagcgcca cattgtgcaa tgccacagcc gtatttggtc ccggttagac cgacattttc
     1141 ccgaatagcc cagagtagcg gtgtgtccgc ttcgacatcg atatcgactg ttttgccgtt
     1201 gatagtgagg tatgccatcg cgttcccctg attggttcaa tccatcgatt gaactagggg
     1261 gcaatgtgtg gccggaaatc tggaacaatt caaatcaaga atctgcgtac gctgccggaa
     1321 gatcatcggt cccgcggtgt tgtcggcgcg gcgtgcctgc gattgccacg atgtgctatc
     1381 gcgtgaccgt catgcgcagg aagctcacca tacttcccag agccgcgaat ccagcaccga
     1441 gcgccagtcc gagcgccgcg ccgttgtggc cggcgagact gaagcacaac gccgcaagtg
     1501 ccgcaccggt ggtctgaccg gtcagacgcg ccgttgccac gatgccgctt gcgctgccgc
     1561 tgcggccggg tggggcgctc gacatcaatg cccgcatgtt gggcgtttga aagaagccga
     1621 aaccaagtcc acagaccccc atgcgccaga cgatgttggc gacgcttggg ctcgccggaa
     1681 gcatcgccag tagcgccatg cccacagcta gcagcgccaa accgatgccg ccgagaatgc
     1741 cggcagggta gcggtcggac agccgcccac cgatcggcgc catgatcgct accacgaggg
     1801 gccagggcgt cataaaaaaa ccggtctcta cctgagatcg ctgcagcacg tcctcgaagt
     1861 agaaaggcaa ggccacgaag gacagtccct ggaccgcgaa cgagcagacg gctgtcaggg
     1921 cggacaacgc gaacaccggc cgccgaaaca ggtcgattgg cagcatcggt gccggatgat
     1981 ccgcatgccg gcgggtcagc atccagccca gcaccaaagc ggcaatcagt tcggcgatca
     2041 ccagcatcgg gggcacctta tgggcggcgc tgccgattcc gataatgaag aggccgagac
     2101 aagccgcggc catgaccgag ccgaagaaat cgaagttgtg cacggcccgc ggcgtcgccg
     2161 gcagtgtttt ccaaccgatc agcatcgcga tcacgccgaa cggcaagttg atggcgaaca
     2221 accacggcca gtggccaatc gccaatatgc cggaagcaat ggttggaccc agcgtgaacg
     2281 cggtggcgac taccaacgcg ttatgaccga atccacgacc cagcatgcgg ctcgggtaaa
     2341 tgaagcgaac caatgccgtg tttacggtca tcaggccgct ggcgccgagg ccctggagtg
     2401 tccgggcaac cagcaagcta tccagcgacc acgcgcacgc gcaacccaat gacgccaatg
     2461 tgaacagcag gagaccgccg agatagatgc gttcgtggcc gacgatttca ccgagcgcgc
     2521 cgagcggcaa cagcgttgcc accagtgcaa tctgatagac attcacaacc cagaccacgt
     2581 cggccgggct gacatgaaga tcgacggcga tcgcgggcag ggcgatattg gcgatcacgg
     2641 tatccagcga ggccatcgtc agcgcggtaa agatggcggc gacagcccat cgccggcgtt
     2701 cccagggcag gccatccatg accggcccgg aaggcggctt ggcggtggtt atatcggtgt
     2761 ccatacccgc agaattctcc tgatcgccat ggcgatggcg ccataagcgg caggtacacc
     2821 agaaaagcgg tgggccccag ccgtgtggct gcatgatgcg tatgcgacgg tccagcgcgc
     2881 cctgcgttat ttccgcccgg taccttttgc tagctttccg tccgtctcgc gatcgactag
     2941 gtttcgcgtt gcactgcgac cgcaaattcc aaaagacggt cggcggcaag gggaagcgtt
     3001 ttgagcggac aggagggcct gggcaccgaa ttgctgcgcg gtggggtaac cccgattggg
     3061 gcggcggatg gcgtggtcat ccgcagcggc aatgagagca atcgccgtcg tctatggacc
     3121 gccctgtggg ttgcgctgtt gctggcgatc ctgggctttc tggtcatcta tcccgttctg
     3181 accctgctgt tgggcgcact gaccgacacc aatccggtgg tcgaaggctt gagcctgagc
     3241 cacctctcga tcgccaattt cattgccgtg ctgatcaacc ccaacgtggc cgaagcgttg
     3301 ttcaacacgc tggtggtctg cggtggtgga accctgatcg cggtggtgat cggactggcg
     3361 ttctcctgga tcgtggtgcg gaccaatacg ccgttcaagg gttttatcgc ggccaccagc
     3421 gtgctgccgc tattcgcgcc gccgctggtc gcgggcgtgg catggaccat cctgggctcg
     3481 ccgaagaccg gcctcatcaa caccatgttc aaatggatgg gtctcgactg gcacgtgaat
     3541 ttctattcgc tgccgggcct cgtgttcgtg ttcggcatct actacgcgcc ctatgtgtac
     3601 atgttcaccg cgtcggcgct gcggaacatg gatccgagcc tggaggaggc agccgaaatt
     3661 tccggcgcca gcgcgttcgc gacgctgttt tcggtcacgt tcccgctgat catgccggcg
     3721 atcatctccg gcatgctgct gtcctttatc gtaatgctcg gcatctacgg cattccggcg
     3781 gtgctcggtg cgccggccaa tatcaacgtg ctgacgacct atatcttcaa gctcaccaac
     3841 tggtcgccgc cgctctataa caccgcggcc gcggtcgcca ttctcctgat gatggtgacg
     3901 ggctttctgg tgttcctgca gcagcgggta ttgagcggac gaagctacac cacggtggcc
     3961 ggcaaggcgt tccggccgcg caacctcaat ctcgggccgt ggcgctggtt tacgctgggg
     4021 ctcgc
//
LOCUS       DASYCS010000311        10194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3709155,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000311 DASYCS010000000
VERSION     DASYCS010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 10194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10194
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3709155"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..581)
                     /locus_tag="VGM46_09735"
                     /pseudo
     CDS             complement(1..581)
                     /locus_tag="VGM46_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010968814.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
     gene            812..1693
                     /locus_tag="VGM46_09740"
     CDS             812..1693
                     /locus_tag="VGM46_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY4192913.1"
                     /translation="MSVGHAGDRLVILGSKGGPAIRPGGPSPSSSLLELGGRGIVVDC
                     GLGVTRGLTDAGMNLKALDLIFITHLHSDHVLELGPLIHTAWTAGLSRPVTVFGPPGT
                     ADYWRHFIRSLQFDIDTRIDDEGRPDIRSLVRVKEFGEGMVLEDGELKVRALRVNHPP
                     VVDCFALRFEHGGRSIVFSSDTAFFPPLAGFAKNADILVHEAMLEEGIDRLVARTGNG
                     VRLRQHLLASHSFAGEAGRIAAEAAVRKLVLNHLIPADDMEIGEADWIEAVRKSWDGE
                     LLVGRDGLVVALAASGS"
     gene            complement(1734..2072)
                     /locus_tag="VGM46_09745"
     CDS             complement(1734..2072)
                     /locus_tag="VGM46_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family sulfur transferase"
                     /protein_id="HEY4192914.1"
                     /translation="MDVRQITDSYSVSPQITLDQIDAIKAAGFKTVICNRPDEEQPGQ
                     PSHDAIKAAVEAAGMAFRYIPVISGQMTMENVGDQAAALEEVEGPVLAYCRSGTRCTN
                     LYAAIEQSKG"
     gene            complement(2274..3203)
                     /gene="aztC"
                     /locus_tag="VGM46_09750"
     CDS             complement(2274..3203)
                     /gene="aztC"
                     /locus_tag="VGM46_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AztC"
                     /protein_id="HEY4192915.1"
                     /translation="MLKTIHAALALSVITLTGFAAPAAFAEPLKVVASFSIIGDLAKN
                     VGGDRVQITTVVGPDGDAHVYEPKPADAVAMTKADVVLVNGLHFEGFLQRLVDASATK
                     AAVVELTKGVTPIEFKPEFAEEDAAPEGGHGVIDPHAFQSIPNAMIYVKNIADAFCAA
                     DSDGCVSYQTNAAAYTKKLETLDGQVRAVIDSIPENKRVVITSHDAFGYFEHTYGLTF
                     LAPQGLSTESEPSAGDVAKLVDQIKQDKAAAIFVENITNPKLIERISSETGIKVGGEL
                     FSDALSPADGPASTYIDMMHNNIAQIKGAIQGS"
     gene            complement(3237..4109)
                     /gene="aztB"
                     /locus_tag="VGM46_09755"
     CDS             complement(3237..4109)
                     /gene="aztB"
                     /locus_tag="VGM46_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease AztB"
                     /protein_id="HEY4192916.1"
                     /translation="MTALFDLFIAPFADFGFMQRALAGSLMLSLGACPVGVFLMLRRM
                     SLSGDAMSHAILPGAAAGFLFYGLEILPMSIGGLIAGVIVALSAGAVSRFTVQREDAS
                     LAAFYLISLAIGVLMVSVRGSSIDLMHVLFGTVLALDNEALGLIGGIVVVTLASLAVF
                     WRALVAECLDPLFLRSVSRLGSPVHFIFLGLVVLNLVGGFQALGTLLAVGLMILPAAG
                     ARFWSARVGPMCVLAVLIGSASCISGLLLSYHASLPSGPAIILSAGVVYFVSILFGTR
                     GIVRDRITHRHRTA"
     gene            complement(4106..4918)
                     /gene="aztA"
                     /locus_tag="VGM46_09760"
     CDS             complement(4106..4918)
                     /gene="aztA"
                     /locus_tag="VGM46_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010915522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein AztA"
                     /protein_id="HEY4192917.1"
                     /translation="MTQTCLAFHDLTLGYASHPAVHHLDGVVRKGSLTAVVGANGSGK
                     STLMKGIAGVLKPMSGRIDRDSGTRIAYLPQQSELDRSFPARVVDLVSLGLWPRRGLL
                     GRHTAEDRQSVSQALTAVGLTGFETRGLDTLSGGQLQRTLFARVLVQDADLILLDEPF
                     NAVDSKTVSDLIALIKRWHGEERTVLVVVHDLDLVRQNFPETLLLARRKVAWDATATT
                     LHAENLLRARRFHEAWEDDAPWCEPEASAHDHHGEERHGAGHHHGIERRAAE"
     gene            5125..5469
                     /locus_tag="VGM46_09765"
     CDS             5125..5469
                     /locus_tag="VGM46_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009363205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY4192918.1"
                     /translation="MSIQRIDVGPRMSGAVVHGNTVYLAGQVGEPGGSVEQQTKDVLA
                     AIDATLAKAGTDKTKILQAIIWLADMSTFAEMNAVWDKWVPQGHTPARATGEAKLATP
                     EYKVEIIITAAI"
     gene            complement(5482..6219)
                     /gene="hutC"
                     /locus_tag="VGM46_09770"
     CDS             complement(5482..6219)
                     /gene="hutC"
                     /locus_tag="VGM46_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019857531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine utilization repressor"
                     /protein_id="HEY4192919.1"
                     /translation="MTTTEVVEGEAQSLHQRILSDISARILSGEWAPGHRIPYEHELS
                     AEYKCSRMTVNKALSQLAKAGLIERRRRSGSFVRQPQSQAAILEIHDIRTEVEALDLP
                     YRYERVGHTRRRSTAHDRSLLELGAAGPVLALDCRHFAGERPFCHEARLISLEAVPEA
                     GAELFLETAPGPWLVGRVPWNAAEHRISAMAVDKRIAAALDIATGAACLVVERRTWNA
                     EGPVTHVRFVYPAESHALVARFTPAQA"
     gene            complement(6232..7584)
                     /locus_tag="VGM46_09775"
     CDS             complement(6232..7584)
                     /locus_tag="VGM46_09775"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HEY4192920.1"
                     /translation="MTAIFAEQALLAEGWRNNVRLTIDGGKIASVEADASALPGDERH
                     AIVLPGMPNLHSHAFQRGMAGLAETRGPSSDSFWSWREVMYRFALSMTPDQVEAVAAQ
                     LYVEMLEAGFSRVGEFHYLHHDLDGRQYANIAEMAERTAAAASQTGIGLTLLPVFYAH
                     SGFGGLEPNEGQRRFINDLNSFERLLEKASEAVRALHQGIVGIAPHSLRAVTPDELAR
                     VVSMTGGPIHIHVAEQVKEVEDCVAWSGARPVEWLLAHAPVDERWCLIHATHMLDGET
                     IAIAKSGAIAGLCPITEANLGDGTFPAALFMQHGGKFGIGSDSNVLIGLPDELRQLEY
                     SQRLFHRARNVLAEPGQSNGRRLFAAALDGGRTALGVGSCQLTAGHSADFASLDASHP
                     SLAGKAGDAILDAWIFANGARVDCVWVHGKKLVEAGRHARRDAIAQRFKAVMKTLSSN
                     "
     gene            7683..8915
                     /gene="hutI"
                     /locus_tag="VGM46_09780"
     CDS             7683..8915
                     /gene="hutI"
                     /locus_tag="VGM46_09780"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEY4192921.1"
                     /translation="MVAHDKTRLEAQRVWRNARLATLAEGLPGLGIVEHGAVVVQDGR
                     IVYAGPEADMPAVSAEAETVDCQGRWITPGLIDCHTHLVYAGNRANEFEMRLAGASYE
                     EVTRAGGGIVSSVKSLRAASEDELVAQTLPRLDALMAEGVTTLEIKSGYGLDLENESK
                     SLRAARRLGNERPVSVRATFLGAHALPPEMNGDKDAYIDLVANRMLPVIAAEGLADAV
                     DGFCEGIAFSTEQIARVFDAAKGAGLPVKLHADQLSNLHGAALAARYGALSADHLEYT
                     DEAGAAAMAKAGTVASILPGAFYFIRETKKPPIDLFRRHGVKMAIATDSNPGTSPLTS
                     LLLTMNMAATLFGLTVDECLAGVTREAARALGVLGQTGTLEAGKSADLAIWDIERPAE
                     LVYRMGFNPLHARIWRGL"
     gene            8912..>10194
                     /gene="hutH"
                     /locus_tag="VGM46_09785"
     CDS             8912..>10194
                     /gene="hutH"
                     /locus_tag="VGM46_09785"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEY4192922.1"
                     /translation="MTELILKPGEATLDDWRAIYRGAVPKLDASCRPKIKASAEAVRR
                     ILAKGEPVYGVNTGFGKLASVRIATGDLETLQRNIVLSHAAGVGEPMPVAIARLMMAL
                     KMASLAQGASGIRPQTIELLEAMLANDVIPVVPAQGSVGASGDLAPLSHMTAAMIGVG
                     ECFTPHGRVPAKVAFVSHGLEPAVLGAKEGLALLNGTQFSTAYALAALFEAEVLYQSA
                     LVAGALSTDAARGSDAPFDPRIHLLRKHRGQIETAQALRNLMAGSAIRESHRVGDERV
                     QDPYCLRCQPQVMGAALTVLRQAADTLRTEANGVTDNPLIFPGDDLGEDTALSGGNFH
                     AEPVAFAADMIALAVCEIGSLSERRLAMLVDPALSGMPAFLTPKPGLNSGFMIPQVTA
                     AALVSENKQKAYPASVDSIPTSANQEDHVSMAAHGA"
BASE COUNT         1912 a         3150 c         3252 g         1880 t
ORIGIN      
        1 atcgccagga tcagcatcat cacccagacc gccggcccag cccaatcctg gaagcggcgc
       61 accgtttcca tgccgcgctg gatgatgagc agttgcaggc cccacatcac cagaaaggtg
      121 atgacctcaa gccccgaatg gccgaggaaa tgggtgttgg catggaaatc cgcaaaccac
      181 tgcgtacgga tcaatagcgc tacgatcgcg cccgatgccg ctgccgtttg cgcgccttac
      241 cagaagcagg cgacgatggc gcgcacgatg gcaggcacgt tggcgcccca tatgccgaac
      301 gaagcccgcg ccagaacagg gtagggcacg ccggtcttga cgccggcata gccgaccatg
      361 ttcatcagag cgtagataat gagcgagccg atgccgatgg cgatgatgaa attgacgaag
      421 ccgccgcaaa acagaaacag gctggcggcg agatagtatc cccacaggct gtgcacgtcc
      481 gacgtccaga cgttgaagat cgagaacggt ccccaatttc gaaccgttgc cggggcaagg
      541 tcctcattat agaggccagg tgatgcgcct tgtattgtca ttttgaactc cccctcgtgc
      601 aggtcgcgcc ctcccgcgcg taatgggtca ggctacgcct tactcaacgg aagcggcaag
      661 catgcgattt gcccggttca gcaggaaaga cattcaatgc agattgcgat tgtcggcacg
      721 gcgatactgg gaagcgagtc gcaggatcgg ccatttttgg agatggccgg aagatggttt
      781 agctggatag tacgctggag gagttgtggt tttgagtgtt ggacatgcag gtgatcggct
      841 ggtcattctc ggctcgaaag gcggccctgc gatcaggccg ggcgggccgt cgccgtcctc
      901 gtcgttgctg gaactgggcg gccgcggcat cgtggtcgat tgcggtttgg gcgtcacgcg
      961 cggcctgacc gacgccggca tgaatttgaa ggcgcttgac ctcatcttca tcacccatct
     1021 gcattccgat cacgttctcg aactcggacc gttgatccac accgcatgga cggcgggcct
     1081 gtcgaggccg gtcacggtgt tcggcccgcc cggaaccgcc gactattggc ggcatttcat
     1141 ccgctcgctc cagttcgaca tcgatacccg tatcgacgac gagggccggc ccgatattcg
     1201 cagcctcgtg cgcgtgaagg aatttggcga aggcatggtg ctggaggatg gcgagcttaa
     1261 ggtccgcgcg ctgcgcgtaa accatccgcc ggtggtggat tgctttgcgc tgcgcttcga
     1321 gcacggaggg cgaagcattg tcttttcatc cgacaccgcc ttctttccgc cgctggcggg
     1381 gtttgcgaag aatgccgata ttctcgttca cgaagcgatg ctggaagaag gcatcgatcg
     1441 gctggtcgcc aggacaggca acggcgtccg gctgcgtcaa catctgctgg cgagccatag
     1501 cttcgccggt gaggcgggcc gcatcgccgc cgaggcggcg gttcgaaagc tggtgctgaa
     1561 ccatctcatt cccgccgacg atatggaaat cggtgaggcc gactggatcg aggcggtccg
     1621 caaaagctgg gacggcgagt tgctggttgg gcgcgacggg cttgtcgtcg cgttggcggc
     1681 gagcggtagc taggaagccg ccgaggaaag gtagcttcct gacggcatgg ccttcagccc
     1741 tttgactgct cgatagccgc gtagaggttc gtgcagcgtg tgccggagcg gcaataggcc
     1801 agcaccggac cctctacctc ttccagcgcc gccgcctggt ccccgacatt ttccatggtc
     1861 atctgaccgc tgatgacggg aatgtagcga aacgccattc ccgctgcttc caccgccgcc
     1921 ttgatggcgt cgtgcgaggg ctggccgggc tgctcttcgt cgggccggtt gcagatgacg
     1981 gtcttgaagc cggctgcttt gatggcgtcg atctggtcga gcgttatctg cggcgaaacc
     2041 gaatagctgt cggtgatctg gcgaacgtcc atggcgttta tccttgcaaa gcttcggcgg
     2101 cagtcatgcc attccgcaac cggatgggca actgccgccg agaatttccc gcccgttaga
     2161 gcggttccga tttttgcagg atcgacaaaa ccgctctatc tctttgtttt ggccgcattt
     2221 ccggacgcaa aaccgtttca cacttttgct ggaaatgctc ttggcgtcgc aggtcagctt
     2281 ccctggatgg cgccttttat ctgggcgatg ttgttgtgca tcatgtcgat ataggtcgag
     2341 gccggaccgt ccgcaggcga gagcgcgtcc gagaagagtt caccgccgac cttgatgccc
     2401 gtctcgctgg aaatccgctc gatcagtttc gggttggtga tgttctcgac gaaaatggcc
     2461 gcggccttgt cctgcttgat ctgatcgacc agcttggcga catcgccggc cgaaggttcg
     2521 gattcggtcg agaggccctg tggcgcgagg aatgtcaggc cgtaggtgtg ctcgaaataa
     2581 ccgaacgcat catgcgaggt gatgaccacg cgcttgtttt cggggatcga atcaatgact
     2641 gcccgcacct ggccatccag cgtttccagc tttttggtat aggcggccgc attggtctgg
     2701 tagctgacgc agccgtcgct gtcggcggcg cagaaggcat cggcaatatt cttcacatag
     2761 atcatggcgt tggggatgga ctggaaggcg tgcgggtcga tcacgccatg accgccctcg
     2821 ggtgcggcgt cttcttcggc aaattccggc ttgaactcga tcggcgtgac acccttggtc
     2881 agctccacga cagcggcctt ggtggcgctg gcatcgacga gcctttgcaa aaagccttcg
     2941 aaatgcaggc cgttgaccag cacgacatcg gccttggtca tggcgaccgc atcggccggc
     3001 ttcggctcgt aaacatgcgc atcgccatcc gggccaacca ccgtggtgat ctggacgcgg
     3061 tcgccgccga cattcttggc cagatcgccg atgatggaga aactggcgac gaccttcagc
     3121 ggttcggcga aggcggcagg cgctgcaaag ccggttaatg ttataacgct cagagcgagg
     3181 gcggcatgaa ttgtcttcag catttggctg tctccttgga agactgggtt tgaagattag
     3241 gctgttctgt gacggtgtgt gatgcggtcg cgaacgatgc cgcgcgtgcc gaacaggata
     3301 gacacgaaat aaacgacgcc cgcggaaagg attattgcgg ggccggatgg cagcgacgcg
     3361 tgataagaaa gcagcaggcc cgaaatgcag gacgccgagc cgattagcac cgccagcacg
     3421 cacatggggc cgacgcgcgc gctccagaag cgtgcgccgg cggccggcag gatcatcagc
     3481 ccgaccgcca gaagcgtgcc gagcgcctgg aagccgccga caaggttgag cacgacgagg
     3541 ccgaggaaaa tgaaatgcac ggggctgccc agccggctga ccgagcgcag gaacaacggg
     3601 tcgaggcatt ccgccaccag agcgcgccag aacacggcga ggctggccag tgtcacgacg
     3661 acgatgccgc cgatcagccc caacgcttca ttgtcgagcg ccagcacggt gccgaacagc
     3721 acatgcatca gatcgatgct ggagccgcgc acggatacca tcagcacgcc gatggccagt
     3781 gaaatgaggt agaaagccgc cagcgaggcg tcttcccgct gcacggtgaa gcgcgagacg
     3841 gcgcccgcgc tcagcgcgac gatgacgcct gcgatcaggc cgccaatgct catcggcagg
     3901 atttcgaggc cgtaaaacag gaagccagca gcggcgcccg gcaaaatggc gtgcgacatg
     3961 gcatcgccgg aaaggctcat gcggcgcagc atcaggaaca cgccgaccgg gcaggcgccc
     4021 agcgacagca tcagcgaacc ggccagcgcg cgctgcatga agccgaaatc ggcaaacggt
     4081 gcgatgaaca ggtcaaacag cgcggtcatt ccgccgcccg ccgctcgatg ccgtgatgat
     4141 gcccggctcc atgacgctct tcgccgtgat ggtcatgtgc ggaggcttcg ggttcgcacc
     4201 acggcgcatc gtcttcccat gcttcgtgga agcggcgcgc gcgcagcagg ttttcagcat
     4261 gcagcgtcgt cgcggtcgcg tcccatgcca ctttccggcg ggccagaagc agcgtttcgg
     4321 ggaaattctg gcgcaccaga tcgagatcgt gaacgacaac caataccgtg cgttcctcgc
     4381 catgccagcg ctttatcagc gcgatcaggt ccgaaacggt cttgctgtcg acggcattga
     4441 atggttcgtc cagcaaaatg agatcggcgt cctgaaccag cacgcgggca aacagcgtgc
     4501 gttgcagttg gccgcctgaa agcgtgtcca ggccgcgtgt ttcgaagccg gtcaacccga
     4561 ccgccgtcaa tgcctggctg acgctttggc gatcctcggc cgtgtggcgg ccgagcaatc
     4621 cacgccgggg ccacaggccg agcgaaacga ggtcgaccac gcgggccgga aacgagcgat
     4681 ccagttcgga ttgttgcggg agataggcga tgcgcgtgcc gctatcgcgg tcgatacggc
     4741 cggacatggg tttcagcacg ccggcgatac ccttcatcag cgtggatttt cccgaaccat
     4801 tggccccgac aacagccgtc agcgaaccct tgcgcacgac gccgtccaga tgatgcacgg
     4861 ccggatggct ggcatagccg agcgtcaggt cgtgaaaggc aaggcaggtc tgcgtcatgc
     4921 tgtccgaagt tcttcttccc cacgcggcga gcgagggtgc gactgtgatg gatgctggat
     4981 atgtgatgtt attacatatg tcaacggcga tacgcatgga aatttggtga tgaccggctc
     5041 cccgtcacag gattccagcg gccggacttg ccctcaagcg ggcccgaccc taaacaggcg
     5101 caaccatcag cgaaaggaac tatcatgagc attcagcgta tcgatgtcgg cccgcgcatg
     5161 agcggcgctg tcgttcacgg caacaccgtc tacctcgccg gccaggtcgg tgagccgggc
     5221 ggttccgtcg agcagcagac caaggacgtc cttgccgcca tcgatgccac gcttgccaag
     5281 gccggcaccg acaagaccaa gatcctgcaa gccatcatct ggcttgccga tatgagcacc
     5341 ttcgccgaga tgaatgcggt gtgggacaaa tgggtgccgc agggccacac cccggcgcgc
     5401 gccaccggcg aggccaagct cgccacgccc gaatacaagg tcgagatcat catcacggcg
     5461 gcgatctagg cccgcaagcc gctaggcctg cgcgggcgta aacctcgcca ccagcgcatg
     5521 gctttcggcg ggataaacga agcggacatg ggtgaccggc ccttcggcgt tccatgtccg
     5581 ccgttccact accaggcatg ccgccccggt cgcaatgtcg agcgcggcgg cgatgcgctt
     5641 gtcgacggcc atggcgctga tgcggtgctc ggccgcgttc cacggcacgc gcccgaccag
     5701 ccacgggccg ggggcggttt cgaggaatag ctcggccccg gcttccggca cggcttcgag
     5761 gctgatcagt cgcgcttcat ggcaaaatgg ccgttcgccg gcgaagtggc ggcagtccag
     5821 cgccaaaacc ggccctgccg cgcccagttc aagcaggctc ctgtcgtgtg cggtgctgcg
     5881 tcttctggtg tgcccgaccc gttcatatcg gtagggcagg tcaagcgcct cgacctctgt
     5941 tcggatatcg tggatttcga ggatggcagc ctgtgattgc ggctggcgaa cgaagctgcc
     6001 ggaacgccgc cgccgctcga tcagcccggc cttggccaac tgggacagcg ccttgtttac
     6061 cgtcatgcgc gagcatttat attccgcgct caattcgtgt tcatagggaa tgcgatgacc
     6121 cggcgcccat tcgccggaaa ggattcgcgc gctgatgtcc gacaggatgc gctgatgcag
     6181 cgattgcgcc tcgccctcca ccacttccgt cgttgtcatc gtcgttcggc ctcagttcga
     6241 ggaaagcgtt ttcatcaccg ccttgaagcg ctgcgctatg gcatcgcgcc gggcatggcg
     6301 tccagcttcg accagtttct tgccatgcac ccacacgcag tcgaccctcg cgccattggc
     6361 gaatatccag gcatcgagga ttgcgtcgcc cgccttgccc gccagcgagg ggtgcgaggc
     6421 gtccagtgat gcgaaatcgg ccgaatgccc cgccgtcaat tggcaagacc cgacgccgag
     6481 tgccgtgcgg ccgccatcga gcgcggcggc aaacagcctg cgtccgttcg actgtccggg
     6541 ttccgcaagc acgttgcggg cgcggtggaa aaggcgctgc gaatattcaa gctggcgcaa
     6601 ctcgtccggc aggccgatca ggacgttgga atcggaaccg atgccaaatt tgccgccatg
     6661 ctgcatgaac aaggccgcgg gaaaagtgcc gtcgcccaga ttggcttctg tgatcgggca
     6721 aagcccggct atggcgccgc tctttgcgat ggctatcgtc tcaccgtcca gcatgtgggt
     6781 ggcgtggata agacaccagc gctcgtcgac cggcgcgtgg gccagcagcc attccaccgg
     6841 ccgcgcgccc gaccatgcaa cgcaatcctc gacctccttg acctgttcgg cgacatggat
     6901 gtggatcgga ccgccggtca tggagacgac ccttgccaac tcatcgggcg tcaccgcgcg
     6961 aaggctgtgc ggcgcgatgc cgacgatgcc ttgatgcaag gcgcgaacgg cctcgctggc
     7021 cttctcaagc agtctttcga aactgtttag atcattaatg aatcggcgct gaccttcatt
     7081 gggctcaagt ccgccaaaac ctgaatgggc ataaaaaacc ggcagcagcg tcaagccaat
     7141 gccggtctgc gaagcagcgg ccgcggtgcg ctcggccatc tcggcgatgt tcgcatattg
     7201 gcggccatcg agatcgtgat gaaggtagtg aaattcgccg acgcgcgaaa agccggcctc
     7261 cagcatctcc acgtaaagct gcgccgcgac cgcctcgacc tggtccggcg tcatcgacag
     7321 ggcgaaacgg tacatcacct cgcgccagct ccagaaactg tcggaggacg ggccgcgcgt
     7381 ctcggccagg ccagccatgc cgcgctggaa ggcgtggctg tgcaggttgg gcatgccggg
     7441 aagcacgatg gcgtggcgct catcgccggg cagggcgctg gcatccgcct cgacagacgc
     7501 gatcttgccg ccgtcgatgg tgaggcgcac attattgcgc cagccttccg ccagaagcgc
     7561 ctgttccgca aagattgccg tcacttttcc ctcccgatga tcgccggtca ttctacaata
     7621 tcgcttgcgc cgccttttga tatgtatata cataatatga gccaattgat acggaaaaga
     7681 tcatggtcgc ccacgataaa acccggttgg aagcacagcg cgtctggcgt aacgcacgtc
     7741 tcgcgaccct cgctgaaggc ctgcccggcc tgggtattgt cgagcatggc gcggtcgtcg
     7801 tgcaggatgg ccgcatcgtc tatgccggac cggaagcgga catgcccgcc gtttctgccg
     7861 aggcggaaac agtcgattgc cagggccgct ggatcacgcc tggcctgatc gactgccaca
     7921 cccatcttgt ctatgcgggc aaccgcgcca acgagttcga gatgcggctg gcaggagcct
     7981 cctatgagga agtcaccagg gccggcggcg gcatcgtatc ctcggtcaag tcgctgcgcg
     8041 cggccagcga ggatgaactc gtcgcgcaga cgctaccgcg cctcgatgcg ctgatggcgg
     8101 agggcgtcac caccctcgag ataaaatccg gctacgggct ggatcttgaa aatgagtcga
     8161 aatcgctgcg cgctgcccgc cggttgggca atgagcgtcc ggtttcggtg cgcgcgacat
     8221 tccttggcgc gcacgcgctg ccgccggaaa tgaatggcga caaggacgcc tatatcgatc
     8281 ttgtcgcaaa caggatgctg ccggtgattg ccgccgaagg gctggcggat gcggtcgatg
     8341 gtttttgcga aggcatcgcc ttttcgaccg agcagatcgc gcgcgttttc gatgccgcca
     8401 agggggcagg cctgccggtg aagcttcatg ccgaccagct gtccaacctg catggcgcgg
     8461 cacttgcggc aaggtacggc gcgttgtcgg ccgatcatct ggaatatacc gacgaggccg
     8521 gcgctgccgc catggccaag gccggcacgg tggcgtccat cctgcccggc gcattctatt
     8581 tcatccgcga gacgaagaag ccgccgatcg acctgtttcg caggcatggt gtgaagatgg
     8641 ccatcgccac cgacagcaat cccggcacct cgccgctgac ctcgctgctt ttgaccatga
     8701 acatggcagc cacgctgttc ggcttgaccg tcgatgagtg cctggccggc gtcacccgcg
     8761 aagccgcccg cgcattgggc gtgctcggcc aaaccggtac gctggaagcc ggaaagtcgg
     8821 ccgatctggc gatctgggac atcgagcgcc cggcggaact tgtttatcgc atggggttca
     8881 acccgctgca tgcccgtatc tggaggggat tatgacggaa ctcatcttga agcccggcga
     8941 agcgacgctg gacgactggc gcgccattta tcgtggtgcg gtgccgaagc tggatgcctc
     9001 ctgccgtccg aagatcaagg caagcgccga agctgttcgg cgcattctgg ccaagggcga
     9061 gccggtctat ggcgtcaata cgggtttcgg taagctggcc agcgtgcgca tcgccaccgg
     9121 cgaccttgaa acgcttcaga ggaacatcgt gctcagccat gccgccggtg tcggcgagcc
     9181 gatgccggtt gccatcgccc gcctgatgat ggcgctgaaa atggcaagcc tggctcaagg
     9241 cgcgtccggc atccgcccgc agaccatcga acttctggaa gcaatgctgg ccaacgacgt
     9301 catcccggtc gtgccggcgc aagggtcggt cggggcttcc ggcgatctcg caccgctgtc
     9361 gcacatgacg gcggcgatga tcggcgtcgg cgaatgcttc acccctcatg gccgtgtgcc
     9421 ggccaaggtg gcgttcgtct cgcatgggct ggagccggcg gtgctgggcg ccaaggaagg
     9481 cctggcgttg ctcaacggca cgcaattctc caccgcttac gcgctggcgg ctttgttcga
     9541 ggcggaggtg ctttaccaat cggcgctggt tgccggcgcg ctttcgaccg acgcggcgcg
     9601 cggttccgat gcaccgttcg atccgcgcat ccaccttttg cgcaagcatc gcggccagat
     9661 cgaaacggcg caggcgctgc gcaacctgat ggccggcagc gccattcgcg aatcgcaccg
     9721 cgtcggcgac gagcgtgtgc aggacccgta ttgcctgcgc tgccagccgc aggtgatggg
     9781 cgcggccctt accgtattaa ggcaggccgc cgatacgctg cgcaccgagg ccaacggcgt
     9841 taccgacaat ccgctgatct tccctggtga cgaccttgga gaagataccg cgctttccgg
     9901 cggcaatttc catgccgagc cggtggcctt cgccgccgac atgatcgcgc ttgccgtctg
     9961 cgaaatcggc tcattgtcgg agcggcggct ggccatgctg gtcgatccgg cgctgtccgg
    10021 catgcctgcc ttcctgacgc cgaagcccgg cctcaactcc ggcttcatga tcccgcaggt
    10081 gacggcggca gcgctcgttt ctgaaaacaa gcagaaggcc tatccggcca gcgtcgattc
    10141 catcccgact tccgccaatc aggaagacca tgtttccatg gccgcgcacg gcgc
//
LOCUS       DASYCS010000312         9406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2694048,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000312 DASYCS010000000
VERSION     DASYCS010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 9406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9406
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2694048"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1198
                     /gene="hemA"
                     /locus_tag="VGM46_09790"
     CDS             <1..1198
                     /gene="hemA"
                     /locus_tag="VGM46_09790"
                     /EC_number="2.3.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012090709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003870 - 5-aminolevulinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5-aminolevulinate synthase"
                     /protein_id="HEY4192923.1"
                     /translation="RIVGKFPRAIWRANGRAQEITVWCSNDYLGMGQHPDVIAALQNA
                     AGQMGSGAGGTRNISGTSNPLVELEMELADLHGKEAGLAFTSGFISNEASISTIARLL
                     PNCLIISDELNHASMIEGVRRSGAEKKIFRHNDVAHLESLLQAAGRDRAKLIVFESVY
                     SMDGDIAPIEKIADLADKYNAMTYIDEVHAVGMYGPRGGGITEREGLADRIDIIEGTL
                     AKAFGTLGGYITGTRAVIDAVRSYAPGFIFTTALPPALAAATTVSIRHLKNSQAEREA
                     QQRQVAHTKHVLAAANLPVMASDTHIVPILVGDPELCKMATDRLLGVHGIYIQPINYP
                     TVPRGTERLRITPTPFHSDQLILDLQGALVETWDALGIPYSGAGRTSVTESDRIIPLL
                     VPKSGG"
     gene            complement(1248..3620)
                     /locus_tag="VGM46_09795"
     CDS             complement(1248..3620)
                     /locus_tag="VGM46_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEY4192924.1"
                     /translation="MNKAGNRRSPFLFPVIGLGAALFLTAGLLIAGQAFGAEGAAEGA
                     AKAPSLSGSSEAAFVAEIVLLLVVGRGLGEILQRWGQPAVMGQLIGGILLGPSLLGWV
                     LPSAQHFVFSSDPVQKSMINAVSQLGILMLLLLTGMETDLKLVRRVRAACFSISTTGV
                     LVPFACGFALAQFLPETLLPEPDERVVAGLFIGTALSISSVKIVAMVVRDMNFMRRNL
                     GQVIVSSAIIEDTVGWLIIAITFGIATHGKVEIIPLLTSIGAVAAFMVFSFTLGRRIV
                     FTLIRWTNDVFRSEYAVITMILIIMGVMALITDMIGVHTVLGAFVAGILVGESPILSD
                     HIESQLRGVITALFMPIFFGMAGLSADLTVLKDPTLALLTLGLVLIASVGKFSGAFIG
                     GKLAGLSVKEATAVGCGMNARGSTEVIVASIGLSMGILSQNLFTMIVTMAVITTLAMP
                     PMLRWALGRLPMGEMEKERVEREELDQRGFVSKLQRLLLAADDSPIGKFTAYLAGLIG
                     GGRGMPTTFLHLANSEAAKPAKSKSPEKHLETIKKGAKASAKAVLESEDTPVDKVQVN
                     ARADTGVSGKTIAEEARKGYDMLLVGLNDSLTAKGDFTARMNDLAGGFDGPLCLVVKG
                     PGTTGRMPTLKAGETILVPVNGTEASRRAAELALAIARPNRARVKALYVAREDVAGQR
                     KTSLSHRREEAVLKDIADLGERYGVPMLTAMRMQGAAGDAIRREAARGIALVVMGVTQ
                     RPGDDLFFGDTAATVLGKSRVPIVLLTTGRVQRDDAEQEARRSASTAPAA"
     gene            3855..4676
                     /locus_tag="VGM46_09800"
     CDS             3855..4676
                     /locus_tag="VGM46_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018448507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEY4192925.1"
                     /translation="MHDRSQTLPHSIAAALRKKAPGFIEFESDAACTTIASYNVHKCV
                     GTDGKFDPARVARVVGEIDADIIALQEADERTGARQGLLDLEALQRETDLVPTHRPEN
                     LGSHGWHGNLILARRGVIQDVRQVRLPGFEPRGAIIVDLEIKGVALRVVAAHFGLLRR
                     SRSQQASALVEATHSVLRPTILLGDLNEWRVRNRSSLLALLPHFGPVHAVLPSFPSRY
                     PLLALDRILARPANLITRIEVHQTPLARLASDHLPIKARLNLDGVRQAPTQPDGA"
     gene            4736..6136
                     /locus_tag="VGM46_09805"
     CDS             4736..6136
                     /locus_tag="VGM46_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019859923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HEY4192926.1"
                     /translation="MDIAASQFARDKIAAGNTGLLLVADNHDAFAARMLAARNATRTL
                     DLMYYLWHDDHTGRVLMQEVVRAAERGVQVRIILDDINPRNSDTAYLALNAHPNIQLK
                     LFNPSGARNGSLLRGVELVFRLFAMTRRMHNKAWIADGAIAIVGGRNIGDAYFDAAET
                     NFRDLDLLVLGAGVRQAAGIFESFWNCPAARPIDALHPGEAISMPGFVASVDEAVDVA
                     LLSATHDLNSISALIAGGDGFRWTSSARVISDPPEKVRGEKPRSWLMQALLPILRSSR
                     RNLEIVSPYFIPGRKGAALFSELVSKGARVTVLTNSLAATDVATVHGAYANYRKRLLR
                     AGIRLFELQPFSRQANISVFGSKGASLHTKAFTVDDRIGFVGSFNFDPRSVSLNAEMG
                     VLFEDEQLVAELRERFRHEIQPQTSYEVTLRDAGLVWTGIDQGQMHQYVREPEASWSR
                     RLLAALVRWLPIESQL"
     gene            6292..7035
                     /locus_tag="VGM46_09810"
     CDS             6292..7035
                     /locus_tag="VGM46_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY4192927.1"
                     /translation="MHGRWRWFLPLPLFLQLSQRDLISNEEKAELFALFSRQKAYAAG
                     DEIVREGARPKVSLLLMEGLAGRFKVLEDGGRQITAFHVPGDFLDLHGFLLKVLAHGV
                     VALSPCTLVQVEHEALKKLTETSPHLARMLWLDTLIDASVHREWLVAMGRRPAIGQLA
                     HLMCELYVRLKVVERTDGYSFTLPLTQNELADALGLSVVHVNRTLRDLRKRGVIRWEG
                     DRIHIDDWDGLVEIAEFDPTYLCLQQEPR"
     gene            complement(7039..7284)
                     /locus_tag="VGM46_09815"
     CDS             complement(7039..7284)
                     /locus_tag="VGM46_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192928.1"
                     /translation="MPRFFFDTYDGEQILSDAEGVEFTGLESVREAAVNALPGIARDR
                     FPNGETMEMWVKVRDAANRPIFEASLIFSSRWIGENG"
     gene            complement(7457..7621)
                     /locus_tag="VGM46_09820"
     CDS             complement(7457..7621)
                     /locus_tag="VGM46_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HEY4192929.1"
                     /translation="MGLGTILIIILILALLGGFSGLGGGPFYGTGYYGGGGLGLVLII
                     VIILVVLGRI"
     gene            complement(7743..8207)
                     /locus_tag="VGM46_09825"
     CDS             complement(7743..8207)
                     /locus_tag="VGM46_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011578731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HEY4192930.1"
                     /translation="MNEVGRARKAIVIDGVALPEILDEAMVRAVVHGFYDAIGSDDLL
                     GPIFHSRIVPDDWPRHLARMCDFWSSTLLRTGRYDGRPLPPHLAIPGLGGQHFRRWLQ
                     LFRATVRRLCPPEVASLFMERALRIAHSFRLAIAFSRSENSVAIAPILEDSL"
     gene            8531..>9406
                     /locus_tag="VGM46_09830"
     CDS             8531..>9406
                     /locus_tag="VGM46_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012709976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEY4192931.1"
                     /translation="MAKVIGIDLGTTNSCIAIMDGKEPKVIENAEGARTTPSIVAISG
                     DGERLVGQPAKRQAVTNPENTIFAVKRLIGRRFDDPVTAKDKDLVPYNIVKGDNGDAW
                     VEAGGKKQSPSQISAMILQKMKETAEAYLGEKVEKAVITVPAYFNDAQRQATKDAGKI
                     AGLEVLRIINEPTAAALAYGLDKREGKTIAVYDLGGGTFDISVLEIGDGVFEVKSTNG
                     DTFLGGEDFDIRLVEYLAAEFKKEQGIDLKNDKLALQRLKEASEKAKIELSASAQTEI
                     NLPFITADASGPKHLT"
BASE COUNT         1787 a         3042 c         2885 g         1692 t
ORIGIN      
        1 gcgcatcgtt ggaaagtttc cccgcgccat ctggcgagcc aatggccgcg cgcaggaaat
       61 caccgtctgg tgctccaacg attatctcgg catgggccag cacccggacg tcatcgccgc
      121 cttgcagaac gcggccggcc agatgggttc gggcgcgggc ggcacgcgca acatttccgg
      181 caccagcaac ccgctggtgg aactggaaat ggaactggcc gacctgcacg gcaaggaggc
      241 cggccttgcc ttcacttccg gcttcatttc caacgaggcg tcgatctcga ccatcgcacg
      301 gcttttgccg aattgcctga tcatctccga tgaactgaac catgcctcga tgatcgaggg
      361 cgtgcgccgc tcgggcgcgg agaagaagat tttccgccac aacgatgtcg cgcaccttga
      421 aagcctgttg caggcggcgg gacgcgatcg cgccaagctg atcgtcttcg agagcgtcta
      481 ttcgatggac ggcgacatcg cgccgatcga gaagatcgcc gacctggccg acaaatacaa
      541 cgccatgacc tatatcgacg aggttcacgc ggtcggcatg tatggcccgc gcggcggcgg
      601 catcaccgag cgcgaggggc tggccgaccg catcgacatc atcgaaggca cgctggccaa
      661 ggcgttcggc acgctgggcg gctacatcac cggcaccagg gcggtcatcg acgccgtgcg
      721 ctcctatgcg ccgggcttca tcttcaccac ggccttgccg ccggcgcttg cggctgcgac
      781 caccgtttcc atccgccatt tgaagaattc gcaggccgag cgcgaagcgc agcagcgcca
      841 ggttgcccat accaagcatg tgctggctgc ggccaatctg ccggtaatgg cgtccgacac
      901 ccacattgtg ccgatcctgg tcggcgatcc cgagctttgc aaaatggcga cggaccggct
      961 gctcggcgtc cacggcatct atatccagcc gatcaactac ccgaccgtgc cgcgcggcac
     1021 ggagcggctg cgcatcacgc cgacgccgtt ccattcggac cagttgatcc tcgatcttca
     1081 aggtgcgctg gtcgagacat gggatgcgct gggcattccc tatagcggcg cggggcgcac
     1141 atccgtcacg gaaagcgacc gcatcatccc gctgctggtt cccaagtcgg gcggctgaac
     1201 gccagccccg tcaggaacgc cagccttgtc aggaacgtgg gcctcgtcta ggccgccggc
     1261 gccgtactgg ccgaacggcg cgcttcctgt tcggcatcgt cgcgctgaac cctgccggta
     1321 gtgagcaaaa cgatcgggac gcggctcttg ccaagcacgg ttgcggccgt atcgccgaag
     1381 aacaaatcat cgcccggccg ctgcgtaacc cccatcacca ccagcgcgat accgcgcgcc
     1441 gcctcgcggc ggatggcgtc gccggcagcg ccttgcatgc gcatggccgt cagcatcggc
     1501 acgccataac gttcgccaag gtcggcgata tccttcagca cagcctcctc gcgccggtgc
     1561 gaaagcgacg ttttacgctg cccggcaacg tcttcgcgcg ccacgtagag cgccttgacg
     1621 cgggcgcggt tgggccgcgc gatggcgagc gccagttcgg cggcgcggcg cgaggcttcg
     1681 gttccgttga cgggaaccag tatggtctcg cccgccttca gcgtcggcat cctgcccgtc
     1741 gttccggggc ctttcaccac caggcaaagt ggcccgtcga acccgccggc aagatcgttc
     1801 atgcgggcgg tgaaatcgcc ctttgccgtc aggctgtcgt tcaggccgac cagcagcatg
     1861 tcgtagccct tgcgcgcttc ctcggcgatg gtcttgccgg aaacgccggt gtcggcgcgg
     1921 gcgtttacct gcaccttgtc gaccggcgtg tcctcgcttt ccaaaacggc cttggcgctg
     1981 gctttggccc ccttcttgat tgtttcgaga tgcttttccg ggctttttga tttggccggt
     2041 ttcgcagcct cgctgttggc gagatgcagg aacgtcgtcg gcatgcccct gccgccgccg
     2101 atcagcccgg ccagataggc ggtgaacttg ccgatcgggc tgtcgtcggc ggccagcagc
     2161 aggcgctgga gcttggagac gaaaccgcgc tggtccaatt cctcgcgctc gacgcgttct
     2221 ttttccattt cgcccatcgg caggcggccg agcgcccagc gcagcatcgg cggcatcgcc
     2281 agcgtggtga tgaccgccat ggtgacgatc atggtgaaca ggttctgcga aaggatgccc
     2341 atcgaaagtc cgatgctggc gacgatgacc tcggtcgagc cacgcgcatt catgccgcag
     2401 ccaaccgccg tcgcctcctt gaccgacagg ccggcaagct tgccgccgat gaaggcgccg
     2461 ctgaatttgc cgacgctggc gatgagaacg aggccaagcg tcagcagcgc cagcgtcggg
     2521 tccttgagca cggtaagatc ggcggaaagg ccggccatgc cgaagaagat cggcatgaac
     2581 agcgcggtga tgacgccgcg cagctggctt tcgatgtggt ctgaaaggat cggcgattcg
     2641 ccgaccagta tgccggcgac gaaagcgcca agcaccgtat gcacgccgat catgtcggtg
     2701 atcagcgcca tcacgcccat gatgatcagg atcatggtga taacggcata ttcgctgcgg
     2761 aatacgtcgt ttgtccagcg gatgagcgtg aagacgatgc ggcgtccaag cgtgaagctg
     2821 aacaccatga acgccgccac cgccccgatg ctggtcagca gtgggattat ctcgaccttg
     2881 ccgtgcgtgg cgatgccgaa ggttatggcg atgatcagcc agcccaccgt gtcttcgatg
     2941 atggcggagg aaacgatgac ctggccaagg ttgcggcgca tgaaattcat gtcgcgaacc
     3001 accatggcta cgatcttgac cgatgaaatc gacaaggcgg taccgatgaa aagcccggcg
     3061 acgacgcgct cgtccggctc cggcagcaag gtttccggca aaaactgcgc cagcgcgaag
     3121 ccgcaggcaa acggcaccag aacgccggtg gtcgagatgg aaaagcaggc ggcgcgcacc
     3181 cggcgcacca gcttcaaatc cgtctccatg ccggtaagca gaagcagcat caggatgccg
     3241 agctgcgaaa cggcgttgat catgcttttc tggaccggat cgctggaaaa cacgaaatgc
     3301 tgcgccgaag gcagcaccca gccaagcagc gaaggcccaa gcaggatgcc gccgatcaac
     3361 tggcccatga cggccggctg cccccatcgc tggaggattt cgccaagccc ccggccgacg
     3421 accagcaaca gcacgatttc ggcgacgaag gcggcttcgc tggaaccgga gagactgggc
     3481 gccttcgccg cgccctctgc tgccccctct gcgccgaatg cctggcccgc gatcaaaagc
     3541 cccgccgtca aaaatagggc cgcaccaagc ccgatcaccg gaaaaagaaa cggcgaccgc
     3601 ctgttgcctg ccttgttcat gaagcgctta tcccctgctt tcctgaccat agaacgcatc
     3661 gaagtggatt tacgttgcgc gtgatcttat tcttttagga acggcatcgt cgccacggtg
     3721 aaaatatccc ggtcaagttc attcatccag ccatccattc actccgggcg cggaaccgcc
     3781 gtctgttcca gccgttgaag aaagcgcccg gcagcccgcc atttcccatc ggcgcaaaac
     3841 cagccacgga cctcatgcac gatcgctcgc agaccctgcc tcattcgatt gccgccgctc
     3901 ttcgaaagaa agcgccgggg ttcatcgagt tcgaaagcga cgccgcctgc acgaccatcg
     3961 cctcctacaa tgtccacaaa tgcgtcggca ccgatggcaa gttcgacccg gcgcgcgtgg
     4021 cgcgcgtggt cggcgaaatc gacgccgaca tcatcgcgct gcaagaggcg gacgagcgca
     4081 ccggcgcgcg tcagggcctg ctcgacctcg aagcactgca acgcgaaacc gacctcgtgc
     4141 ccacccaccg gccggaaaat ctcggaagcc atggctggca tggcaatttg atattggcgc
     4201 ggcgcggcgt catccaggat gtgcggcagg tgcggctgcc cggtttcgag ccgcgcggcg
     4261 cgatcatcgt cgacctcgaa atcaagggcg tcgcgcttcg cgtggtggcg gcgcatttcg
     4321 ggctgctgcg gcgctcgcgc tcgcaacagg caagcgcgct tgtcgaggcc acccattcgg
     4381 tattgcgccc gacgatcctg cttggcgacc tcaacgaatg gcgcgtgcgc aaccgctctt
     4441 cgcttctggc gctgctgccg catttcgggc cggtccatgc cgtgcttccg agctttccgt
     4501 cgcgctatcc gctgctggcg ctcgaccgca tccttgcccg cccggccaat ctcatcaccc
     4561 gcatagaggt gcaccagacg ccgctcgccc gccttgcctc cgaccatctg ccgatcaagg
     4621 cgcgcctcaa cctcgacgga gtgagacagg cgccgacgca accggatggc gcctgaacag
     4681 ttcttgctca acgttgactg cgcgtgctga atagcgccgc agcggatgga tatggatgga
     4741 tatagccgcc tcgcaatttg ccagggacaa gatcgcggcc ggcaataccg gcctgcttct
     4801 ggtcgccgat aatcacgacg cctttgcggc gcggatgctg gcggcgcgca atgcgacgcg
     4861 cacgctcgac ctgatgtact acctctggca tgacgaccat accggccgcg ttttgatgca
     4921 ggaagtcgtc agggcggcgg agcgcggcgt gcaggtgcgc atcattctgg atgacatcaa
     4981 cccgcgcaac agcgacaccg cctatctggc gctcaatgcc catccgaaca tccagctgaa
     5041 gcttttcaac ccgagcgggg cgcgaaacgg cagcctgctg cgtggcgtgg aactggtttt
     5101 ccgtctgttc gccatgacgc gccggatgca caacaaggcc tggatcgccg acggcgccat
     5161 cgccatcgtc ggcggccgca acattggcga cgcctatttc gacgccgccg agaccaattt
     5221 ccgcgacctc gacctgctgg tgctcggcgc cggcgtgcgg caggccgccg gaatcttcga
     5281 atcgttctgg aactgcccgg ccgcccggcc catcgacgcg ctgcatcccg gcgaagccat
     5341 ctcgatgccg ggtttcgtcg cttccgtcga cgaggcggtg gatgtggccc tgttgagcgc
     5401 cacccacgac ctcaattcga tttcggcgct gattgccggc ggcgacggct tccgctggac
     5461 gagcagcgcc cgcgtcatct cggacccgcc cgaaaaggtg cgcggcgaga agccgagaag
     5521 ctggctgatg caggcgctgc ttcccattct caggtcgagc cggcgcaatc tggaaatcgt
     5581 gtcgccctat ttcattcccg gaaggaaggg cgccgccctc ttttcggaac tggtctcgaa
     5641 gggcgccagg gtaacggtgc tgaccaattc tctcgccgcc accgatgtcg ccaccgtcca
     5701 cggcgcttac gccaattacc gcaagcgcct gttgcgggcc ggcatccgtt tgttcgagct
     5761 gcaaccgttc agccggcagg ccaacatctc cgtcttcggc tccaagggcg ccagcctcca
     5821 caccaaggcc ttcacggttg acgacaggat cggcttcgtc ggctcgttca atttcgaccc
     5881 gcggtcggtt tcgctgaacg ccgaaatggg cgtgctgttc gaggacgaac agctggtggc
     5941 ggaactgcgc gagcgctttc gccacgaaat acagccgcag accagctatg aggtgacctt
     6001 gcgggacgcc ggcctggtct ggaccggcat cgaccagggc cagatgcatc aatacgtccg
     6061 cgagccggag gcaagctggt cccggcgcct gctggcggcg ctggtgcgct ggctgccgat
     6121 cgaatcccag ctgtgagcgc aagccgcggg gatggtgatt aagctaccgg tgattaagac
     6181 gctggctttc gctgtggatg cgcgtacgat taacaattga taatgcgcgc gccagccaat
     6241 tggcgttagg caaccgcgcc gcttgttgcc gtcgggggga tccttcctgc gatgcatggt
     6301 cgctggagat ggtttttgcc ccttcctctc ttcttgcagc ttagccagcg cgacctcatc
     6361 tccaatgagg agaaggcgga gcttttcgcg ctgttttccc gccagaaggc ctatgccgcc
     6421 ggcgacgaga tcgtgcgcga aggagcgcgg cccaaggtga gcctgctttt gatggaggga
     6481 ttggccgggc gcttcaaggt gctggaagat ggcggccgcc agataacggc attccacgtt
     6541 cccggcgatt ttctcgacct gcatggcttt ttgctgaaag tgctggcgca tggtgtcgtc
     6601 gcgctttcgc cgtgcacgct ggtccaggtc gagcacgagg cgctgaagaa actgaccgaa
     6661 acctcgccgc atctggcgcg catgctgtgg ctggatacgc tgatcgacgc ctcggtccac
     6721 cgcgaatggc tggtcgccat ggggcgaagg ccggccatcg gccagttggc tcatttgatg
     6781 tgcgagcttt acgttcgcct gaaagtcgtc gaacggacgg atggctacag cttcacgctg
     6841 cccttgaccc agaacgagct ggccgatgcg ctcggcctgt cggtggtcca tgtcaaccgc
     6901 acgctacgcg acctgcgcaa gcgcggcgtc attcgctggg agggcgaccg cattcacata
     6961 gacgattggg acggactggt cgaaatcgcc gaattcgatc cgacctatct atgcttgcag
     7021 caggagccgc gctgagcgtc agccgttttc gcctatccag cgcgatgaaa agatcagcga
     7081 agcctcgaat atcggccggt tggcggcatc acgcaccttt acccacattt ccatggtttc
     7141 gccgttcgga aaccgatcgc gcgcaatgcc gggcagcgcg ttgaccgcgg cttcacgcac
     7201 gctttccagt cctgtgaatt cgacgccttc ggcatcgctc aatatttgct cgccgtcata
     7261 agtgtcgaag aagaagcgtg gcatcgtctc gctccgattg cagaccaatc cacaacagcg
     7321 cgcccaaggc tttggttccg ctgtcgcaaa gtggcggggc taaaagcccc gacaagacaa
     7381 aaacccgccg ctcctttgga acggcgggtt gtctgtccgg cggaaaacgg ctttgccgtt
     7441 acatgccgga ggcttcctat attcgcccaa gcacgacgag gatgatgacg atgatgagga
     7501 ccaagccaag gccgccgccg ccataatagc ccgtgccgta gaatgggccg ccgccaaggc
     7561 cgctgaagcc gccgagaagc gccaggatca gaatgatgat gagaatggtg cctaagccca
     7621 taatgttgcc tcctgctttg cagcgcgcct caatggcgtc agccgttaca tgcgatgaac
     7681 gcggcaaggg gccttcgcgt tccacaactc gttaagcggc aggctttcgg ggcaggcggc
     7741 ccttacaggc tgtcttcgag aataggcgcg atggcgaccg aattctcgct tcggctgaag
     7801 gcgatggcca ggcggaacga atgggcgatg cgcaacgcgc gttccatgaa cagcgatgcc
     7861 acttcgggcg ggcatagcct gcgcacggtg gcgcgaaaca gctgaagcca gcgccggaaa
     7921 tgctgtccgc caaggccggg tatggccaga tgcgggggca ggggccgccc gtcatagcgc
     7981 ccggttcgca gcagcgtcga cgaccagaag tcgcacatcc tggccagatg ccgtggccag
     8041 tcgtccggta ctatccggct gtggaagatc ggcccgagca ggtcgtcgga gccgatggcg
     8101 tcatagaaac cgtggacgac ggcgcgaacc atggcttcgt ccagaatttc cggcagcgca
     8161 acgccgtcga tgacgatcgc tttcctggcc cggccaactt cgttcatttc cgtttcccgc
     8221 aattctcggc cgtgccgtct tcgctgcccg tcttgcctcc acataggctt ctttttgcgc
     8281 gacctcgtcc cggcaacccc ggaccgggcg cggccgggcg tatttgcgca atagcaaact
     8341 ggggaacgtc ctgtcgcgcg cttaatcact cgcgcagaat gcttgcgcgc tggcgagcct
     8401 gttcttatat acgcgccaag ccgttcgacg cttggcctgt gctagtcgtc gcgacggaaa
     8461 tcttgtgaac aggggttttc aacggaacgc ctcatgggtc cggaaaacct gcttagcgga
     8521 gagagtacac atggctaaag taatcggcat cgacctcggc acaaccaatt cctgcatcgc
     8581 catcatggat ggcaaggaac cgaaggtcat tgaaaacgcg gaaggcgcgc gcaccacgcc
     8641 ttccatcgtc gcgatcagcg gcgacggcga acgcctcgtc ggccagccgg ccaagcgcca
     8701 ggcggtcacc aatcctgaaa acaccatctt cgcggtcaag cgccttatcg gccgccgctt
     8761 cgacgacccc gtgaccgcca aggacaagga ccttgtgccc tataatatcg tcaagggcga
     8821 caatggcgat gcctgggtgg aagccggcgg caagaagcag tcgccaagcc agatttcggc
     8881 gatgatcctg caaaagatga aggaaacggc tgaagcctat ctcggcgaga aggtcgagaa
     8941 ggccgtcatc accgttccgg cctatttcaa cgacgcccag cgccaggcca ccaaggacgc
     9001 cggcaagatc gccggacttg aagtgctgcg catcatcaac gagccgactg cggcggccct
     9061 tgcctacggc ctcgacaagc gcgaaggcaa aaccatcgcg gtctatgacc ttggcggcgg
     9121 caccttcgat atttccgtgc tggaaatcgg cgacggcgtc ttcgaggtga agtccaccaa
     9181 tggcgacacc ttccttggcg gcgaggactt cgacatccgg ctcgtcgaat atctggcggc
     9241 cgagttcaag aaagaacagg gcattgacct gaagaacgac aagctcgccc ttcagcgcct
     9301 caaggaagcc tccgagaagg ccaagatcga actgtccgcc agcgcgcaga cggaaatcaa
     9361 cttgcccttc atcaccgccg atgcgtccgg ccccaagcac ctgacg
//
LOCUS       DASYCS010000313         3315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2694799,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000313 DASYCS010000000
VERSION     DASYCS010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2694799"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VGM46_09835"
     CDS             <1..235
                     /locus_tag="VGM46_09835"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEY4192932.1"
                     /translation="SMMPENAGVLTIEFKVNLLSPGKGERFLFRGSVTKPGRTIIVAD
                     GQAYAFAADGEARLIATMTGTMMTVVGRDGIDG"
     gene            238..879
                     /locus_tag="VGM46_09840"
     CDS             238..879
                     /locus_tag="VGM46_09840"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006205020.1"
                     /note="Enables the cleavage of the glycosidic bond in both
                     5'-methylthioadenosine and S-adenosylhomocysteine; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEY4192933.1"
                     /translation="MGEATLLSGKHVLFVMAAQAEYGPHLKDLFAPLMTGVGPVEAAV
                     TLGAELARLAARQALPDLVVSLGSAGSRTLEQGQVYQAVSVAYRDMDASALGFERGVT
                     PFLDLPATLALGPMIPGVREATLSTGAAIVSGAAYDGIAEQMVDMETYACLRACQLFG
                     VPLIALRGISDGAAELRHIGDWTAYLDIIDRKLADAVRLLEKAIAEAWALAAI"
     gene            1195..2757
                     /gene="guaA"
                     /locus_tag="VGM46_09845"
     CDS             1195..2757
                     /gene="guaA"
                     /locus_tag="VGM46_09845"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019443373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEY4192934.1"
                     /translation="MKTANHPDTVLIIDFGSQVTQLIARRVREAGIYSEVVPFQLADE
                     AFRRIKPKAIILSGSPHSALDIGSPRAPESIFQAGVPVLGICYGEQTICAQLGGKVEA
                     GHEREFGRAFLEIEDDCALFDGVWAKGTRHQVWMSHGDRVTAIPPGFKVVGTSTGAPF
                     AAIADEARKFYAVQFHPEVVHTPDGAKLLRNFVHIIAGLKPDWTMAAYREQAVEAIRK
                     QVGKGKVICALSGGVDSSVAALLIHEAVGDQLTCILVDHGLMRKDEAAGVVAMFREHY
                     NLPLILVNAQDRFIGALEGESDPEKKRKTIGRLFIEVFEEEAQKLGGADFLAQGTLYP
                     DVIESVSFTGATSSTIKSHHNVGGLPERMNMQLVEPLRELFKDEVRVLGKELGLPESF
                     IGRHPFPGPGLAIRCPGGITREKLEILREADAVYLDEIRKAGLYDAIWQAFAVLLPVQ
                     TVGVMGDGRTYEFVCALRAVTSVDGMTADFYPYDMAFLGRAATRIINEVRGINRVVYD
                     VTSKPPGTIEWE"
     gene            complement(2964..3140)
                     /locus_tag="VGM46_09850"
     CDS             complement(2964..3140)
                     /locus_tag="VGM46_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006206788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192935.1"
                     /translation="MQNEPTLTEDESKDEFFERLSKISEEMVARHGKDFSMGALVLAA
                     RWIAENRDGSLKTN"
     gene            complement(3173..3298)
                     /locus_tag="VGM46_09855"
     CDS             complement(3173..3298)
                     /locus_tag="VGM46_09855"
                     /inference="COORDINATES: protein motif:HMM:NF024298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192936.1"
                     /translation="MVKPLGGAITALTWSGDGRHLAVGCADGAAAIVTFPAQMFK"
BASE COUNT          583 a         1059 c         1024 g          649 t
ORIGIN      
        1 ttcgatgatg ccggaaaatg ccggcgtgtt gaccatcgaa ttcaaggtca acctgctgtc
       61 gcccggcaag ggcgaacgct tcctgtttcg cggctcggtg accaagcccg gccgcaccat
      121 catcgtcgcc gacggacagg cttatgcctt tgcggccgat ggcgaggcgc ggctgatcgc
      181 caccatgacc ggcaccatga tgaccgtggt ggggcgcgac ggcatagacg gctgaccatg
      241 ggcgaggcga ccctgctttc cggaaagcat gtgcttttcg tcatggcggc gcaggccgaa
      301 tatgggccgc acctgaaaga tttgtttgcg ccgctcatga cgggcgttgg cccggtcgag
      361 gccgcggtga cgcttggcgc ggaactcgcc cggctggccg cgcgacaagc cttgcccgac
      421 ctcgtcgttt cgctcggctc cgccggcagc cggacgctgg aacaggggca agtttatcaa
      481 gccgtctcgg tcgcctatcg cgacatggat gcgtccgccc tcggctttga gcgcggcgtg
      541 acgcctttcc tcgacctgcc ggcgacgctt gccctggggc cgatgattcc gggcgtgcgc
      601 gaggcgacgc tttcgacagg cgcggccatc gtctccggcg ccgcctatga cggcattgcc
      661 gagcagatgg tcgacatgga aacctatgcc tgcctgcgcg cctgccagct gtttggcgtg
      721 ccgctgattg cgttgcgcgg catttcggat ggtgcagccg agcttcgcca tatcggcgac
      781 tggaccgcct atctcgacat catcgaccgc aagctggccg atgccgtccg cctgctggag
      841 aaggcgattg ccgaggcttg ggcactagcg gcgatatgat ttcccgatga cgattcaacg
      901 agccgagccc catatcgttg cccgttttgt tgcccgcaac agttataaat tatccgctcc
      961 gggcaaaccc gatattgcat gaaaagaggc tggctttggc ctgatcgcgg caacaaactg
     1021 cgcggcaacg gatgaaccgc gccgttcttg ccgccatcgc catgccggcg gcacaggtgg
     1081 cggcgctagt ccatttgccg gagccgtcac gctttggccg gccaggcgca tctgcgacgt
     1141 caggcccatg acaaacccat tgcgtcgagc gtacttcttc tttaaaggcc cgccatgaag
     1201 acagccaatc atcccgatac cgttctcatc atcgatttcg gcagccaggt cacccagctg
     1261 atcgccaggc gcgtccgcga agccggcatc tatagcgagg tcgtgccgtt ccaattggcc
     1321 gacgaggcgt tccgccggat aaagcccaag gcgatcatcc tttccggcag cccccattca
     1381 gccctcgaca ttggcagtcc gcgcgctccc gaatccatct ttcaggccgg cgttcccgtt
     1441 cttggcatct gctacggcga acagaccata tgcgcacagc ttggcggcaa ggtagaagcc
     1501 ggccatgaac gcgaattcgg ccgcgccttc ctcgaaatcg aggacgattg tgccctgttc
     1561 gacggcgtct gggccaaagg cacccgccat caagtgtgga tgagccatgg cgaccgcgtc
     1621 accgccattc cgccgggctt caaggtcgtc ggcacctcga ccggcgcacc ctttgcggcg
     1681 attgccgacg aggcgcgcaa attctatgcg gtgcagttcc accccgaagt ggtgcacacg
     1741 ccggacggcg ccaagctgct gcgcaatttc gtccacataa tcgccggcct gaagcccgac
     1801 tggaccatgg ccgcctatcg cgagcaggcg gtcgaggcta tccgcaagca ggtcggcaag
     1861 ggcaaggtca tctgcgcgct ttcgggcggg gtcgattctt ccgtggccgc gctgctgatc
     1921 cacgaagcgg tcggcgacca gcttacctgc atcctcgtcg accacggcct gatgcgcaag
     1981 gacgaggccg ccggcgtcgt ggccatgttc cgcgagcact ataatctgcc gctgatcctg
     2041 gtgaacgcgc aggaccgttt catcggcgcg ctggaaggcg agagcgatcc ggaaaagaag
     2101 cgcaagacca tcggcaggct gttcatcgag gtgttcgagg aagaggcgca aaagcttggc
     2161 ggggcggatt tcctggcaca gggaacgctt tatcccgacg ttatcgagag cgtttccttt
     2221 accggcgcga cgtcctcgac gatcaagtcg catcacaatg tcggcggcct gcccgagcgc
     2281 atgaacatgc agctggtcga gccgctgcgc gaactgttca aggacgaagt tcgcgttttg
     2341 ggcaaggaac tcggcctgcc ggaaagcttc atcggccgcc atcccttccc cggaccggga
     2401 ctggccatac gctgtccggg cggcatcacc cgcgagaagc tggagatcct gcgcgaagcc
     2461 gatgccgtct acctggacga aatccgcaag gccggcctct acgacgccat ctggcaggcc
     2521 ttcgccgtgc tgctgccggt gcagacggtc ggcgtgatgg gcgatgggcg cacctacgaa
     2581 ttcgtctgcg cgctgcgcgc cgtcacttcg gtcgatggca tgacggccga cttctaccct
     2641 tacgacatgg ccttcctcgg ccgcgccgcc acccgcatca tcaacgaagt acgcggcatc
     2701 aaccgcgtcg tctacgacgt gaccagcaag ccacccggta cgatcgagtg ggaatgattt
     2761 gataccgtca ggtgactatc gccggtcatc gcgatgggca gacggcacgt cgtaaacaac
     2821 ggtgtcggtt tcgtcgacta ccgggttcca tcgcggcaag cgcctcgatt tgacgtaagc
     2881 cggcggtaaa gtttcttact cagtcgctca ttttaccgtc agccggattt gacgcgcggg
     2941 cgcgggtcaa atccggcttg cgttcaattg gtctttaggg aaccgtctcg attttcggcg
     3001 atccagcggg cagcgagtac cagtgcgccc atgctgaagt ccttgccgtg cctggcgacc
     3061 atttcttccg aaatcttgga gagccgttcg aaaaactcgt ccttgctttc gtcctcggtg
     3121 agggtgggtt cgttttgcat tttttcctcc ggtttcatgc gggtttccgc ggttatttga
     3181 acatctgcgc gggaaaagtg acgattgcgg cggcgccgtc ggcgcaaccg acggccaggt
     3241 gccgtccgtc gcccgaccag gtcagcgccg tgatcgcgcc gcccagcggc ttgaccagaa
     3301 gttcgtcctg cgagc
//
LOCUS       DASYCS010000314         9513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1971021,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000314 DASYCS010000000
VERSION     DASYCS010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 9513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9513
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1971021"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /locus_tag="VGM46_09860"
     CDS             <1..921
                     /locus_tag="VGM46_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013891825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY4192937.1"
                     /translation="GVVAVLFALNFILPDYDQNLFSRIMVLAVFAMGYNLLFGYVGLL
                     SLGHSMFFSAGLYGAGLAVAHLGWGVPAAFAAGLLCGAALALAVGLLALRTTGVAFMI
                     VTMMFAQAFFLTILYFSAWTGGDQGVVVAQDARSVEVGPLALDLTGPVTRYMAALALF
                     SIALLVTLAVVRSRHGRVLAAIRENEERTRMLGYDTFANKLAAVVLSGAICAAAGAAY
                     ALLFGYVGSNFASVQYSILPLLWVLLGGAGTTLGPLVGTLAMYYVIDFTSFYTSAYLL
                     VVGVALIVLVLFFPKGILGTIRQRWLEWVP"
     gene            918..1646
                     /locus_tag="VGM46_09865"
     CDS             918..1646
                     /locus_tag="VGM46_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4192938.1"
                     /translation="MKPLLTTAGLSRDFGGLRAVDNVYFSLAPGEIRAIIGPNGAGKT
                     TFVSLVCGRIQPSAGTIAFDGANITSLPAHRRVRLGIAYTFQITSVFGRLSAYDNVAL
                     AVQRTLGDGRGEAALRAGVMEVLGRVGLADRAAMPADQLAYGHQRLLEVAMGLALKPR
                     LLILDEPTQGLAVAEIENFIALVRDIAKSATVLLIEHNMQVVMQLAHRITVMNAGRIL
                     AEGTPEAIRANAAVQDAYLGKADG"
     gene            1639..2346
                     /locus_tag="VGM46_09870"
     CDS             1639..2346
                     /locus_tag="VGM46_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008598138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4192939.1"
                     /translation="MAEALSIAALDCFYGEVQVLHGLDLTLQQGEVLCLLGRNGAGKT
                     TTLKAIMGLVRAAAGSIKLDGRELTALPAHEVPRAGIGYVPQGRRLFAEMTVAENLEI
                     GLMARGKGRQTREAVLDLFPLLRERLKQRSGTLSGGEQQMLAMARALCLEPQVLLLDE
                     PTEGLMPSMIEKIRETVARLRQAGVSTILVEQRVDAVLSLADRVTFIENGHSRETIAV
                     EALRADPSAIRRYVGVS"
     gene            complement(2405..3805)
                     /locus_tag="VGM46_09875"
     CDS             complement(2405..3805)
                     /locus_tag="VGM46_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018425712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEY4192940.1"
                     /translation="MTFLVAALCSSAALAAEEHGLPGASMGLWWALPFAGLLLCIATG
                     PLLFHHFWEHHYGKIAAFWAVSVIVPLAASFGASAATEAVLHALLLEYMSFIILLFGL
                     FTIAGGILIAGNIHGTPLVNAGLLLIGSILASIVGTTGAAMIMIRPIIRANDNRPFNA
                     HVVVFFIFLVANIGGSLTPLGDPPLFIGFLRGVDFFWTTVHILPDTLFVGGMVLAIFF
                     ILDVILHRREAGAPKIKDPTPDRKVRIRGLVNVPLLAGVIAAILLSATWKPGISLSIH
                     GVGVELQNLVRDLIILTLAFISLAVSRKEYRKANGFEWGPIAEVAKLFAAIFICIVPV
                     VAILKAGHDGALSPLVALVTLPGGQPNDMAYFWLTGILSSFLDNAPTYLVFFELAGGD
                     PQHLMTEFASTLAAISAGAVFMGANTYIGNAPNFMVYAIARRQGVTMPSFFGYMLWSG
                     TVLIPVFILSSLLFFG"
     gene            complement(3882..4481)
                     /gene="pncA"
                     /locus_tag="VGM46_09880"
     CDS             complement(3882..4481)
                     /gene="pncA"
                     /locus_tag="VGM46_09880"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015241029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HEY4192941.1"
                     /translation="MATEALIIVDLQNDFCPGGALAVAGGDEIVPLVNAMIRDIEHVV
                     LTQDWHPAGHSSFASSHPGSQPFQTITMPYGEQTLWPDHCIQGSLGSDFHSGLAWAKA
                     ELVIRKGFRPEIDSYSAFFENDRETPTGLAGYLRERGISAITLAGLATDFCVAFSALD
                     AVAQGFAVTVRLDACRGIGLDGSMDTMLVRMREAGVTLT"
     gene            complement(4657..4881)
                     /locus_tag="VGM46_09885"
     CDS             complement(4657..4881)
                     /locus_tag="VGM46_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019169948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit delta"
                     /protein_id="HEY4192942.1"
                     /translation="MSPDKLIYQVNQIAWFFKSKPRAEGVAGVADHINKFWEPRMRRQ
                     FFEMIDAGVEGFEPLVIEAAPNVRPVEPAN"
     gene            complement(4871..5701)
                     /gene="fdhD"
                     /locus_tag="VGM46_09890"
     CDS             complement(4871..5701)
                     /gene="fdhD"
                     /locus_tag="VGM46_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEY4192943.1"
                     /translation="MASPRAAVTEISRLAHRASGTARANRRVPEETPVAFSFAGTTHA
                     VMMASPADFEDFALGFSLTEGIIASPVEVEAIEVEDHGAGIDIQILLKDGANTRFEAR
                     RRRLAGPVGCGLCGIESIEEAMRSVDAVDASPLSLSQDDIVRSVRLLSKVQPLHAETG
                     AVHAAGFYVPGKGIVMAREDVGRHNALDKLAGALARAGVDGRSGAVVVTSRVSVEMVQ
                     KTAAIGSPFIIAVSAPTALAIRTAAEAGMTLVALVRGEEFDTFTHPDRIASGAAKNVA
                     "
     gene            complement(5706..8576)
                     /gene="fdhF"
                     /locus_tag="VGM46_09895"
     CDS             complement(5706..8576)
                     /gene="fdhF"
                     /locus_tag="VGM46_09895"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007822296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HEY4192944.1"
                     /translation="MDLIQEIDFGTPASKAEQQVTLIVDGKSVTVPAGTSIMRASMEA
                     GVEIPKLCATDMLDAFGSCRVCLVEIEGRNGTPASCTTPVGEGMVVHTQTERLGAIRR
                     GVMELYVSDHPSGHGETRGTGGSEFDHVAELVGMTENRYGTDGRNHIAPGEGSLPVDF
                     IAKDETNPYFIYDPSQCIVCSRCVRACEDVQGTFALTIEGRGFDSRVSASMHESFIES
                     ECVSCGACVQACPTDALREKLVLEHGMPDRSVVTTCAYCGVGCNFKAEMKGEEVVRMM
                     PYKAGKANHGHSCVKGRFAYGYATHRDRILSPMIREKITDPWREVSWEEAMAYTAGEF
                     RRIQYQYGRGSIGGITSSRCTDEETYLVQKLVRQGFGNNNVDTCARVCHSPTGYGLNT
                     TLGTSAGTQDFDSVEETDVMLLIGANPTDAHPVFGSRMKQRLREGAKIIVIDPRRIDL
                     VKTPHIEAAYHLPIKPGTNVAILTGLAHVIVTEKLYDEVYIHSKCDWEAFEDWAEFVS
                     DPRYSPEETAKVCGIEADLVRQAARLYATGGNGAIYYGLGVTEHSQGSTSVMAIANLA
                     MATGNLGRRGVGVNPLRGQNNVQGSCDMGSFPHEFPGYRHVSTDSVRKIYEDLWGVTL
                     DKEPGLRIPNMLDAAVEGTFKGLYVQGEDILQSDPDTKHVAAGLAAMECVVVHDLFLN
                     ETARYAHVFLPGSTFLEKDGTFINAERRINLIRKVMEPKARYADWEATQEMARALGLD
                     WNYTHPSQIMDEIASTTPTFSGVSFELLEREGSVQWPCNEAAPLGTPIMHIDGIVRGK
                     GNFVRTEYVATDEKVGPRFPLLLTTGRILSQYNVGAQTRRTGNIAWHEKDRLEIHPHD
                     AEDRGIRDGDLIRLSSRSSDTTLRALITDRVAPGVVYTTFHHPETQANVITTDFTDWA
                     TNCPEFKVTAVQVAPSNGPSEWQVEYEELSRKTRRIAVEAAE"
     gene            complement(8587..>9513)
                     /locus_tag="VGM46_09900"
     CDS             complement(8587..>9513)
                     /locus_tag="VGM46_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003567172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEY4192945.1"
                     /translation="IATGATKGFIYIRSEYPHAVSIMRRAVEVARRSGVLGVNVLGSA
                     HAFDMEVRVGAGAYVCGEETSLLDSLEGKRGQVRAKPPLPAHKGLFGRPTVINNVISL
                     ASVPIVMDRGAQFYKDYGIGRSRGTIPLQIAGNVKYPGLYEAAFGLTLGEIVDGIGGG
                     TASGRPVKAVQVGGPLGAYFPRELFDTPFDYEAFAAKDGLIGHAGIVVFDDSADMLKQ
                     ARFAMEFCAIESCGKCTPCRVGSVRGVETLDKLATGIEPEKQIALVTDLCNTMKFGSL
                     CALGGFTPYPVMSALTHFPEDFRPTPVAEAAE"
BASE COUNT         1665 a         2991 c         3144 g         1713 t
ORIGIN      
        1 ggcgtcgtcg ccgtcttgtt tgcgctgaat ttcatcctgc cggattatga ccagaacctg
       61 ttttcccgca tcatggtgct ggcggtgttc gccatgggct ataatctgct gttcggctat
      121 gtcgggctgc tcagccttgg ccactccatg ttcttctccg ccggcctcta cggcgcgggg
      181 ctggcggtgg cgcatctcgg ctggggcgtg cccgccgcct tcgccgccgg gcttttatgc
      241 ggcgcggcat tggcgctggc ggtcggcctg ctggctttgc gcaccactgg cgtcgccttc
      301 atgatcgtca ccatgatgtt cgcgcaggcg tttttcctga ccattctcta cttctcggcg
      361 tggaccggcg gcgatcaggg cgtggtggtg gcgcaggacg cgcgtagcgt cgaggtcggc
      421 ccgctggcgc tcgacctgac cggcccggtt acccgctaca tggcggcgct tgccctgttt
      481 tccatcgccc tgctggtcac gctggccgtg gtgcgctcgc gtcatggccg cgtattggcg
      541 gcgatccgcg agaatgagga acgcacgcgg atgctgggct acgacacgtt cgccaacaag
      601 ctcgcggcgg tggttctgtc cggcgcaatc tgcgccgccg ccggtgcggc ctacgcgctt
      661 ttgttcggct atgtcggctc caatttcgcc tcggtgcaat attcgatcct gccgcttttg
      721 tgggtgctgc tcggcggcgc gggcaccacg cttgggccgt tggtcggcac gcttgcgatg
      781 tattacgtca tcgatttcac cagtttttac acctcggcct acctgctggt cgtcggtgtc
      841 gcgctcatcg ttctggtgct atttttcccc aaaggcatac tgggaaccat tcgccagcgc
      901 tggctggagt gggtgccatg aaaccgctgc tgacaaccgc cggcctgtcg cgcgatttcg
      961 gcggcctgcg cgcggtggac aatgtctatt tctcgctggc tccgggcgaa attcgcgcca
     1021 tcatcggtcc gaacggggcc ggcaagacca ccttcgtcag cctcgtctgt ggccgtatcc
     1081 agccttcggc cggcacgatc gcctttgatg gcgcgaacat aaccagcctg ccggcccatc
     1141 gccgcgtccg gctgggcatc gcctatacgt tccagatcac cagcgtcttt ggccggctga
     1201 gcgcctatga caatgtcgct ttggcggtgc agcgaacgct gggcgacggg cgcggcgagg
     1261 ccgcgttgcg ggccggcgtg atggaagtgc tcggccgtgt cggccttgcc gaccgcgccg
     1321 cgatgcctgc cgaccagctt gcctatggcc accagcgcct gctcgaagtg gcgatgggat
     1381 tggcgctgaa gccgcgcctg ctcatcctcg acgagccgac gcaggggctg gcggtcgcgg
     1441 aaatcgaaaa ctttatcgcc ctcgtccgcg acatcgccaa aagcgcgacc gtgctgctga
     1501 tcgagcataa tatgcaggtg gtgatgcagc ttgcccaccg catcaccgtg atgaatgccg
     1561 gcaggatact ggccgaaggc acgcctgaag ccatccgcgc caacgccgcc gtgcaggatg
     1621 cctatctggg gaaggccgat ggctgaggcg ctgtccattg ccgcgctgga ctgcttctac
     1681 ggcgaagtgc aggtgctgca cggcctcgac ctgaccttgc agcagggcga agtgctgtgc
     1741 ctgctcggac gcaacggcgc cggcaagacg acgacactga aggcgatcat ggggctggtg
     1801 cgggccgccg caggctcgat caagctggat ggccgggaac tgaccgcgct acctgcgcat
     1861 gaggtgccaa gggccggcat cggctatgtg ccgcagggca ggcggctgtt cgccgaaatg
     1921 acagtggccg aaaacctgga aatcggcctg atggcgcgcg gcaagggcag gcaaacgcgc
     1981 gaagccgtgc tcgatctgtt tccgctgttg cgcgagcggc tgaagcagcg ttcaggcacg
     2041 ctgtcggggg gcgagcagca gatgctggcc atggcgcgcg cgctctgcct ggagccgcag
     2101 gtgctgctgc tggacgagcc gaccgaaggg ctgatgccgt cgatgatcga aaagatccgc
     2161 gagacggtgg cgcggctgcg gcaagccggc gtttccacca ttctggtcga gcagcgggtc
     2221 gatgcggtgc tgtcgcttgc cgaccgcgtg accttcatcg agaacggcca tagccgcgag
     2281 acgatcgccg tcgaagcgct gcgcgccgac ccgtccgcca tccggcgtta tgtcggcgta
     2341 agctgacccg attccgggaa agcgcaagcc ggattgccgt ccgcaatcgt gtggtaaagc
     2401 tgtttcagcc gaagaaaaga agactagaca ggatgaatac cgggatcagc accgtgcccg
     2461 accacagcat atagccaaag aagctcggca tggtcacgcc ctgccgccgc gcgatggcat
     2521 agaccatgaa gttgggagcg ttgccgatgt aggtgttggc gcccatgaag accgcgcccg
     2581 ctgaaattgc cgccagcgtc gaggcgaact cggtcatcag gtgctgcggg tcgccgccgg
     2641 ccagttcgaa gaacaccaga taggtcggtg cgttgtcgag gaacgacgac aggatgccgg
     2701 tcagccagaa ataagccatg tcgttgggct ggccgccggg cagggtgacg agcgccacca
     2761 gcggcgacag cgcgccgtca tggccggctt tgaggatcgc caccacggga acgatgcaga
     2821 tgaagatggc ggcgaacagc ttcgccactt cggcgatagg cccccattcg aagccgttgg
     2881 ccttgcggta ttccttgcgc gatacggcca gcgagatgaa ggcgagcgtc aggatgatga
     2941 ggtcgcgcac caggttctgc aactcgacgc cgacgccatg gatgctcagc gaaatgccgg
     3001 gcttccaggt ggcggacaaa aggatcgcgg caataacgcc tgccagcaac ggcacattga
     3061 cgaggccgcg aatgcgaacc ttgcggtcgg gcgtcgggtc cttgatcttc ggcgcgccgg
     3121 cttcgcggcg atgcaggatg acatccagga taaaaaatat ggcaagcacc atgccgccga
     3181 cgaacagcgt gtcgggcagg atgtgaacgg tcgtccagaa gaaatcgacg ccgcgcagga
     3241 agccgatgaa caagggcgga tcgccgagcg gggtcagcga gccgccgata ttggcgacga
     3301 ggaagatgaa gaacacaacg acatgggcgt tgaagggccg attgtcgttg gcgcggatga
     3361 tcgggcggat catgatcatc gccgcgccgg tcgtcccgac gatcgaggcg aggatcgagc
     3421 cgatcagcag caggccggca ttgaccagcg gcgtgccgtg gatattgccc gctatcagga
     3481 tgccgcctgc gatggtgaac agcccgaaca acaagatgat gaacgacata tattcaagca
     3541 gcagcgcgtg aagcaccgcc tccgtggcgg cggatgcgcc gaaggaggcg gccagcggaa
     3601 cgatgaccga cacggcccag aaagcggcga tcttgccgta gtgatgctcc cagaagtgat
     3661 ggaaaagcag cgggccggtg gcgatgcaca aaagcaggcc ggcaaaaggc agcgcccacc
     3721 acagccccat cgaggcgccg ggcaagccat gctcctcggc cgccagcgcc gcgcttgagc
     3781 atagcgcggc gacaaggaac gtcatgcccg ccagggccac cttcgaaagc cgcatcaaac
     3841 ccccatccga tatttctgcg cgcgaacccg cgcggtgttg ctcaagtaag cgtcacgccc
     3901 gcctcgcgca tgcgcaccag cattgtgtcc atcgatccgt cgaggccgat accccggcag
     3961 gcgtcgagcc ggaccgtcac agcgaacccc tgcgcgacag cgtccagcgc ggagaaggcg
     4021 acgcagaaat cggtggcaag cccggcaagc gtgatcgccg agatgccgcg ttcgcgcaga
     4081 tagccggcaa gccctgtcgg cgtttcgcgg tcgttctcga aaaaggccga atagctgtcg
     4141 atttcagggc gaaagccctt gcggatgacc agttccgcct tcgcccaggc gaggcccgaa
     4201 tgaaaatcgg aaccgaggct accctgtatg caatggtccg gccacagcgt ctgctcgcca
     4261 taaggcatcg tgatcgtctg gaacggctgg ctgccgggat ggctggaagc gaagctcgaa
     4321 tggccggcgg gatgccagtc ctgcgtcagc acgacatgct cgatgtcacg gatcatcgcg
     4381 ttgaccagcg gcacgatctc gtcgccgccg gcaaccgcaa gcgcgccgcc ggggcaaaaa
     4441 tcgttctgca agtcgacaat gatcagcgct tccgtggcca tgaagccaaa tctcctgttt
     4501 ccggcaggcc gcgaagcggc cggggcagaa acttgaccag atggccggcc acgtcaacct
     4561 tcaaccggga atggcagcct caaagcagag tgacgccgtt tgcaggggat tgtccggtca
     4621 gcagacgggt ggggccgcgc caacggctgg tcggtcctag tttgccggct caaccggcct
     4681 gacgttgggc gcggcttcga tgaccagcgg ctcgaaaccc tcgacgccgg catcgatcat
     4741 ttcgaagaac tgccggcgca tgcgcggttc ccagaacttg ttgatgtggt ccgccactcc
     4801 ggcaacgccc tcggcgcgcg gcttcgactt gaagaaccac gcgatctggt tgacctggta
     4861 gatcagcttg tcaggcgaca tttttcgcgg ctccggaagc gatgcggtcg ggatgggtga
     4921 aggtatcgaa ttcctcgccg cgcaccagcg ccaccagcgt catgccggcc tccgcggcgg
     4981 tgcggatggc aagcgccgtc ggcgcggaca cggcgatgat gaagggcgaa ccgatggccg
     5041 ccgtcttctg caccatttcc accgagacgc gcgacgtgac gacgaccgcg ccggaccgcc
     5101 cgtcgacccc ggccctggcc agcgcgccgg ccagcttgtc gagcgcgttg tggcggccga
     5161 catcctcgcg cgccatgacg atgcccttgc cgggaacata gaaccctgcc gcatgcaccg
     5221 cgccggtctc ggcgtgcagg ggctgcacct tcgacaaaag ccttacggaa cgcacgatat
     5281 cgtcctgtga aagcgatagc ggcgaggcat cgaccgcatc gaccgaccgc atggcttcct
     5341 cgatggattc gatgccgcac aggccgcagc ccacggggcc ggccagtctg cggcggcgcg
     5401 cctcgaaacg cgtattggcg ccgtccttca gcaatatctg gatgtcgatg ccggcgccgt
     5461 gatcttcgac ctcgatggct tcgacctcga caggcgaggc gatgatgccc tcggtcagcg
     5521 aaaaacccaa tgcgaaatcc tcgaaatcag ccgggcttgc catcatcacc gcatgggtgg
     5581 tgccggcgaa actgaacgct accggcgttt cctccggcac cctgcggttg gcccttgccg
     5641 tgccgctggc ccggtgggcc agccgcgata tttcggtgac ggcggcgcgt ggcgaggcca
     5701 ccggcctact ccgccgcttc gaccgcgatg cgccgggtct tgcgggacag ttcctcatat
     5761 tccacctgcc attcgctcgg cccgttcgag ggcgcgacct gcacggcggt caccttgaat
     5821 tcggggcagt tggtcgccca gtcggtgaag tcggtggtga tgacgtttgc ctgcgtttcc
     5881 gggtggtgga aggtggtgta gaccacgccg ggcgctacgc ggtcggtgat cagcgcgcgc
     5941 agcgtcgtgt cgctggagcg gctcgacagt cggatcaggt cgccgtcgcg gatgccgcgg
     6001 tcctcggcgt cgtgcgggtg gatttccagc cggtcctttt catgccaggc gatgtttccg
     6061 gtacgcctgg tctgcgcgcc gacattgtac tggctgagga tgcgaccggt ggtgagcagc
     6121 agcgggaagc gcggcccgac cttctcgtcg gtcgccacat attcggttcg cacgaagttg
     6181 cccttgccgc gcacgatgcc gtctatgtgc atgatcggcg tgcccagcgg cgccgcttcg
     6241 ttgcatggcc actgcaccga gccttcgcgc tcaagcagtt cgaaggagac gccggagaaa
     6301 gtaggcgtcg tcgatgcgat ctcgtccatg atctgcgagg gatgcgtata gttccagtcg
     6361 aggccgagcg cgcgggccat ttcctgcgtt gcctcccagt cggcatagcg ggccttcggc
     6421 tccatcacct tgcgtatcag attgatgcgc cgctcggcat tgatgaaggt gccgtccttt
     6481 tccaggaagg tcgagccggg caggaacaca tgcgcatagc gcgccgtctc gttgaggaac
     6541 aggtcgtgca ccaccacgca ttccatcgcg gccaggccgg cggcgacatg cttggtgtcc
     6601 ggatcggatt gcaggatgtc ttcaccttgc acgtagaggc ccttgaaggt gccttcgacg
     6661 gcggcgtcga gcatgttggg aatgcgcagg cccggctcct tgtcgagcgt cacgccccac
     6721 aaatcctcgt agatcttgcg cacggaatcg gtcgagacat gccggtagcc cggaaattcg
     6781 tgcgggaacg accccatgtc gcacgatccc tgcacattgt tctggccacg cagcgggttc
     6841 acgccgacgc ccctgcggcc gagatttcct gtcgccatcg caaggttggc gatggccatg
     6901 accgaggtcg atccctggct gtgctcggtg acgccgaggc cgtaatagat cgcgccattg
     6961 ccgccggtcg cgtagaggcg cgcggcttgg cggaccaggt cagcctcgat gccgcagacc
     7021 ttggcggtct cttccgggct gtagcgcgga tcggagacga attccgccca gtcctcgaag
     7081 gcttcccagt cgcatttgga atggatgtag acctcgtcat agagcttttc ggtgacgatg
     7141 acatgggcga ggccggtcag gatggcgacg ttggtgcccg gcttgatcgg caggtgatag
     7201 gcggcctcga tatgcggcgt cttgaccagg tcgatgcggc gcggatcgat cacgatgatc
     7261 ttggcgcctt cgcgcagccg ctgcttcatg cgcgagccga acaccggatg cgcgtcggtc
     7321 ggattggcgc cgatcagcag catcacatcc gtctcctcga cggaatcgaa gtcctgcgtg
     7381 cccgccgagg tgccaagcgt ggtgttgagg ccatatccgg tcggcgaatg gcagacgcgc
     7441 gcgcaggtgt cgacattgtt gttgccgaag ccctgccgca ccagcttctg gacgagatag
     7501 gtttcctcat ctgtgcagcg cgacgaggta atgccgccga tcgagccgcg cccatattgg
     7561 tactggatgc gccggaactc gccggcggtg taggccatgg cctcttccca cgacacttcg
     7621 cgccagggat cggtgatctt ctcgcggatc atcgggctga ggatgcggtc gcggtgcgtg
     7681 gcgtagccat aggcaaagcg gcccttgacg caggaatggc catgattggc cttgcccgcc
     7741 ttgtagggca tcatgcgcac gacttcttcg cccttcatct cggccttgaa gttgcagccg
     7801 acgccgcaat aggcgcaggt ggtgacgacc gagcggtcgg gcatgccgtg ttccagcacg
     7861 agcttctcgc gcaaagcgtc ggtcgggcag gcctgcacgc aggcgccgca ggatacgcat
     7921 tcggattcga tgaagctctc atgcatgctg gccgaaacgc gggaatcgaa gccgcgcccc
     7981 tcgatggtca gcgcgaaggt gccctgcacg tcctcgcagg cccgcacgca gcgcgagcag
     8041 acgatgcact gggacggatc gtagatgaaa tatgggttcg tttcgtcctt ggcgatgaaa
     8101 tcgaccggca gcgaaccttc gccgggcgca atgtggttgc ggccatcggt gccgtagcgg
     8161 ttttccgtca tgccgaccaa ttcagctaca tgatcgaact cgctgccgcc ggtgccgcgt
     8221 gtctcgccat ggccgctcgg atgatcggaa acgtaaagct ccatgacgcc gcgccggatc
     8281 gcgccgaggc gctccgtctg cgtatggacg accatgcctt cgcccaccgg cgtggtgcag
     8341 gaggcgggcg tgccgttgcg gccttcgatc tcaaccaggc agacgcggca ggagccgaac
     8401 gcatccagca tgtcggtggc gcacagcttg ggaatttcga cgcctgcttc catcgaagcg
     8461 cgcatgatcg aggttcccgc cggcacggtg acggacttgc cgtcgacgat cagggtgacc
     8521 tgctgctccg ccttggaggc gggcgtgccg aaatcgattt cctggatgag atccatgccg
     8581 gttctcctat tctgccgctt cggcgaccgg ggtcggccga aaatcttcgg ggaaatgggt
     8641 aagcgcgctc atcaccggat atggggtgaa accgcccaat gcgcaaagcg agccgaattt
     8701 catagtattg cacaaatcgg tgaccagcgc gatctgcttc tccggctcga tgcccgtggc
     8761 cagcttgtcg agcgtctcca cgccgcgcac cgaaccgacc cggcatggcg tgcacttacc
     8821 gcagctttcg atggcgcaga actccatggc gaagcgcgcc tgcttcagca tgtcggcgct
     8881 gtcgtcgaag acgacgatgc cggcatggcc gatcagcccg tccttggcgg cgaacgcctc
     8941 gtagtcgaac ggcgtgtcga acagttcgcg cgggaaataa gcgcctagcg gcccgccgac
     9001 ctgcaccgcc ttgaccgggc ggccgctggc ggtgccgccg ccgatgccgt cgacgatttc
     9061 gcccagcgtc aggccgaacg ccgcctcgta aagccccgga tatttcacgt tgccggcgat
     9121 ttggagcgga atggtgccgc gcgagcggcc tatgccgtaa tccttgtaga actgcgcgcc
     9181 cctgtccatg acgatcggca cggaagccag cgaaatgacg ttgttgatga cggtcggccg
     9241 gccaaacagg cccttatgcg ccggcagcgg cggcttggcg cgcacctgcc cgcgcttgcc
     9301 ttcaagcgaa tcgagcagcg acgtttcctc gccgcagaca taggcgcccg cgccgacgcg
     9361 cacttccatg tcgaaggcgt gggcggaccc cagaacattg acgccgagca cgcccgagcg
     9421 gcgcgccact tcgaccgccc ggcgcatgat ggacaccgca tgcggatatt cggagcgaat
     9481 gtagatgaag cccttggtcg cgccggtggc gat
//
LOCUS       DASYCS010000315         2687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3421005,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000315 DASYCS010000000
VERSION     DASYCS010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3421005"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..1128
                     /locus_tag="VGM46_09905"
     CDS             223..1128
                     /locus_tag="VGM46_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006200454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY4192946.1"
                     /translation="MSKGKNVYKDAYNRCLRLLDETRQLPSEPELGTALGVSRTTVRA
                     ILGRMQETGLIEWNKRDKTVLRAPVEQDFFPEEETDTLSQVIERSFMRRLLEGGAEAG
                     IQINELELAREIGVGTTSVREFLIRFSRFGLIEKRRNSHWVLKGFTRSFALELTEIRE
                     MFELRSAAAFARLPVDNPAWAALDALEAEHRALQKSIATRFSEFSELDERFHRLIHSA
                     SHNRFIVDFYDVIAMIFHYHYQWNKAGERERQRNEAAVGEHLVYIEALKSRDAAKVDS
                     ACRMHLKSARRTLLTSMPEAAGAEG"
     gene            1253..1609
                     /locus_tag="VGM46_09910"
     CDS             1253..1609
                     /locus_tag="VGM46_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006200453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192947.1"
                     /translation="MAAAFAAAYLLVLQSVVGAFALGVGPSPAQLDAFGNIICTHDGT
                     GKLPDGGPQQRHMPNCCVLGCALASSALCTPPAEACRIELRSSFHAIDYLPAAPAHLA
                     FERDRSPANPRAPPAA"
     gene            1708..2268
                     /locus_tag="VGM46_09915"
     CDS             1708..2268
                     /locus_tag="VGM46_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEY4192948.1"
                     /translation="MFQSSHHNGGRTHSLSASFLHGVEQRLGLGLFVLALLFAGAHAV
                     FAHGFKVGDIEIEHPWSRESPMGAHVAGGYLTITNKGASADRLISITSDISAKAELHQ
                     MAVSDGVMTMREVPGGLEVPAGGSVAFDTGGYHLMFVDLKRQPKKGEHFAATLTFEKA
                     GPVTVEFAVEGMGETGGMDMDADHAK"
     gene            2306..>2687
                     /locus_tag="VGM46_09920"
     CDS             2306..>2687
                     /locus_tag="VGM46_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1775 domain-containing protein"
                     /protein_id="HEY4192949.1"
                     /translation="MLKFLTAAGVAGVLIAAGTPGAFAHITLETQEAAVGSTYKAVLR
                     VPHGCEGKATTAVRVKMPEGFIAVKPMPKPGWKLETVKGKYAKSYTLWGEAMSEGVTE
                     VDWSGGNLPDEFYDEFVLRGTLTDS"
BASE COUNT          504 a          806 c          870 g          507 t
ORIGIN      
        1 ctgctggctg cgctcatggt cgaaatcctg ggtcgaaatc ctgggccgaa atgctgggac
       61 gaattcctgg atggcgcgcc cctggatggc gcgccgttga caggcgaaat ggtctgggta
      121 atgtctttta tcgggaaaaa acatttaccc gtcaaccggt ttttcacgta ggaagcgctc
      181 gaagttttgc ttatcgccgg ccaggccggt aacggggaag gcatgtcgaa gggaaaaaac
      241 gtctataagg atgcctataa ccgttgcctg cggctgctcg acgagacgag gcaactgcct
      301 tcggagccgg aactgggcac cgcgcttggc gtcagccgta ccacggtgcg cgccatactc
      361 ggccgcatgc aggagacggg gctgatcgag tggaacaagc gcgacaagac cgttttgcgt
      421 gcgccggtgg aacaggattt ctttcccgag gaagaaaccg acacgctgtc gcaggtcatc
      481 gagcgtagtt tcatgcggcg gctgctggag ggcggcgcgg aagccggcat ccagatcaac
      541 gagcttgagc tggcgcgtga aatcggcgtc ggcaccacca gcgtgcggga attcctgatc
      601 cgcttcagcc gcttcggctt gatcgaaaag cgccgcaaca gccattgggt gctgaaaggg
      661 tttacgcgct ccttcgcgct ggaactgacc gaaatccgcg agatgttcga actgcgctcg
      721 gctgcggcct tcgcccggct gccggtggac aatccggcat gggcggcgct ggacgcgctg
      781 gaggccgaac accgcgcgct gcaaaagtct atcgccaccc gcttcagcga attctccgaa
      841 ctggacgagc ggtttcaccg gctcatccac agcgcgtcgc ataaccgctt catcgtcgat
      901 ttctacgatg tcatcgcgat gatctttcac tatcactatc agtggaacaa ggcaggcgag
      961 cgcgagcgcc agcgcaacga agcggcggtg ggcgagcatc tggtctatat cgaggcgctg
     1021 aagtcgcgtg acgcggccaa ggtcgacagc gcctgccgca tgcacctgaa atcggcgcgc
     1081 cgcacgcttt tgacctcgat gcccgaggcg gcgggggccg agggttgaag tgtcgcgctt
     1141 cattgtcgcg gcggcggctt ggcgatattc cgccggcgat tgccgtgcta gaaatcggcc
     1201 tgttgtcgat tggagggatg cggcgttgag cagtcggcgc agacgatgga gcatggcagc
     1261 ggcattcgcc gcagcctatc tgctcgtcct gcaatcggtt gtcggcgcct tcgcgctcgg
     1321 cgtcgggcca agtccggcgc agttggacgc cttcggcaac atcatctgta cccatgacgg
     1381 gacaggcaaa ctgccggatg gcggcccgca gcaaaggcac atgcccaatt gctgcgtgct
     1441 tggctgcgcc ttggcttctt cggcgctctg tacgccgccg gcggaagcgt gccgcatcga
     1501 gcttcgctcg tcgtttcacg cgatagacta tctgcctgcg gcaccggcgc atcttgcgtt
     1561 cgaacgcgac aggtcgccgg ccaatccgcg cgcgccgccc gccgcctgat tggcccgccg
     1621 ccggcggaat ctttcgccgg caccctcatc caataactga attttcgcga ccgccaggcg
     1681 gcgcgaatcc gcatcgacgg agctatcatg tttcaatctt cccatcacaa tggcgggcgt
     1741 acgcactcgc tttccgcctc cttcctgcac ggcgtcgagc agcgactcgg ccttggcttg
     1801 ttcgtgctgg cgctgttgtt cgccggcgcc catgccgtct ttgcgcatgg gttcaaggtc
     1861 ggcgatatcg agatcgaaca tccgtggtcg cgcgaatcgc cgatgggcgc gcatgttgcc
     1921 ggcggctacc tcaccatcac caacaagggc gcttcggccg accggctgat ctcgatcact
     1981 tccgacattt cagccaaggc cgaacttcac cagatggcgg tcagcgacgg cgtcatgacc
     2041 atgcgggaag tgccgggcgg cctcgaagtg cctgcgggcg gttcggtggc gttcgacacc
     2101 ggcggctatc atctcatgtt cgtcgacctg aagcggcaac ccaagaaggg cgagcacttc
     2161 gcggccacgc tgaccttcga aaaggcaggg ccggtcacgg tcgaatttgc cgtcgagggg
     2221 atgggcgaga cgggtggcat ggacatggac gccgaccatg ccaaatgacg ccagcagttc
     2281 aaaagcaata tttcaggggc gaaagatatt gaaatttttg accgccgcgg gtgtagccgg
     2341 cgttcttatc gccgccggta caccgggtgc tttcgcccat atcacgctgg aaacgcagga
     2401 agctgcggtc ggctcgacct acaaggcggt gcttcgcgtg ccgcatggct gcgaaggcaa
     2461 ggcgacgacc gccgtgcgcg tgaaaatgcc ggaggggttc atcgcggtga agccgatgcc
     2521 gaagcccggc tggaagctgg aaaccgtgaa aggcaaatat gccaagtcct acacgctgtg
     2581 gggcgaggcg atgagcgaag gcgtcaccga ggtggattgg agcggcggca atttgccgga
     2641 cgaattctat gacgaattcg tcttgcgcgg cacgctcacc gacagcc
//
LOCUS       DASYCS010000316         3442 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_521012,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000316 DASYCS010000000
VERSION     DASYCS010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521012"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            342..1349
                     /locus_tag="VGM46_09925"
     CDS             342..1349
                     /locus_tag="VGM46_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HEY4192950.1"
                     /translation="MTASPAVPHPPGITDIRRAAERLNGLAVETPLIESAVLNERFGA
                     RILFKPEVLQRTGSFKFRGAYNKISSLSPQERSRGVVAFSSGNHAQGVAASAAMFGIK
                     AVIVMPSDAPAMKIGNVRAMGGEVVLYDRFGDDRAALVQPFIDKGMAMVPPYDDPAII
                     AGQGTIGLELVAQAAKLGVDLDAVFIPCGGGGLTSGISVAVKDGSPATTIWAAEPEYF
                     DDTRRSLAAGTQIANEPGHASICDALLTPEPGVITFEINRKTLAGSIAVSQKAVAAAM
                     RDAMAHLKLVVEPGGCVALAALSSGEIDIKGKSVAVVLSGGNVDYGLYADIIAAKPPY
                     D"
     gene            1473..2399
                     /locus_tag="VGM46_09930"
     CDS             1473..2399
                     /locus_tag="VGM46_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006326337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4192951.1"
                     /translation="MQPIISISGVSKTYASGFSALKTVNLEINRGEIFALLGPNGAGK
                     TTLISIVCGIVNRSSGTVTVDGHDIGTDYRAARGLIGLVPQELTIDAFETVWSTVNFS
                     RGLFGKPANPAFVEKVLRDLSLWEKKDAKAITLSGGMKRRLMIAKALSHEPRVLFLDE
                     PTAGVDVELRQDMWAMVRRLREDGVTIILTTHYIEEAQAMADRVGVINQGEIILVEGK
                     DELMHKLGRKRLMLELRKPLETVPASLARYELELTADGTQATYTYDDQSDRPGVASLL
                     RDLGAEGIEFRDLDTKNSSLEEIFVNLVRKDA"
     gene            2396..3157
                     /locus_tag="VGM46_09935"
     CDS             2396..3157
                     /locus_tag="VGM46_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4192952.1"
                     /translation="MNPRAIWAIYRQEMARAFRTLLQSIVSPVISTSLYFVVFGSAIG
                     SRITEVDGISYGAFIVPGLIMLSLLTQSISNASFAIYFPKFVGSIYELLSAPVSYLEI
                     VIAYVGGAATKSIVLGLIILATAALFVPLHIEHPFWMLGFLVLTAVTFSLFGFIIGIW
                     AGSFEQLQLVPLLIVTPLTFLGGSFYSIDMLPGIWRTITLFNPVVYLVSGFRWSFYGK
                     ADVSIGVSLGMTVVFLLACIAIVSWIFKTGYRLRN"
BASE COUNT          616 a         1081 c         1044 g          701 t
ORIGIN      
        1 gtttcggacc atggcggccg agctgaagtt gaacatcctg ggcggaagcg gtgctgaagg
       61 ccacaggaac gctggtaaag gcaacggcca gagcaagggc ggttgaggta acaaaactgc
      121 gcatggtcga cactcctttt ccaagccata acgccgacgg ggcgcgttcg gttcaaatca
      181 gatttgaata gcatctggat atactccgtg atgctaaaat aagttcggca gccctgcccg
      241 tgcgttgaat gggcgtcgat ccgcggccga ttttccgttg gcgcgccgtt atgatttacg
      301 tgcaaatggg tgttcgatga ttcccgcttc aggaggctcg cgtgaccgcc agtccagccg
      361 taccccatcc ccccggcatc accgatatcc gccgcgctgc cgaacgcctc aacggacttg
      421 ccgtcgagac cccgctgatt gaatctgcgg tgctcaacga acgtttcggc gccagaatcc
      481 tgttcaagcc ggaggtgttg caacgcaccg gctcgttcaa gttccgcggc gcctacaaca
      541 agatctcctc gctttcgccg caagagcgca gccgcggcgt cgtcgccttc tcgtcgggca
      601 atcacgcgca gggcgttgcc gcttccgccg ccatgttcgg catcaaggcg gtcatcgtca
      661 tgccgtccga cgcacccgcc atgaagatcg gcaatgtccg cgccatgggc ggcgaggtgg
      721 tgctctacga ccgtttcggg gacgaccgcg ccgcccttgt ccagcctttc atcgacaagg
      781 gcatggcaat ggtgccgccc tatgacgacc ccgccatcat cgccgggcag ggcaccattg
      841 gccttgaact ggtggcgcag gcggcaaagc ttggcgtcga tctcgacgcg gtgtttatcc
      901 cttgcggcgg cggcggcttg accagcggca tttccgttgc cgtgaaggat ggttcgccgg
      961 cgaccaccat ctgggcggcc gagcctgaat atttcgacga tacccgccgt tcgctggcag
     1021 ccggcacgca gattgccaat gagcccggcc atgcctccat ctgcgatgcg ctgttgacgc
     1081 cggaacccgg cgtgatcacc tttgaaatca accgcaaaac cctggccggc agcatcgccg
     1141 tttcgcaaaa ggcggttgca gccgcgatgc gcgacgccat ggcccatctg aagctggtgg
     1201 tggagccggg cggctgcgtg gcgcttgccg ccctgtcgtc gggcgagatc gacatcaagg
     1261 gcaaaagcgt tgccgtggtg ctttccggcg gcaatgtcga ttacggcctc tatgccgata
     1321 tcatcgccgc aaaaccgccc tacgattgag cctgtttggc cggaaatgtc ggcagaggcc
     1381 cggaaatctc ggctctggca ttgacttccg gcaaatcgaa ccgacattca gcggacttta
     1441 accgaagctt ttttccagca gcagttcatt ccatgcagcc aatcatttcc atttccgggg
     1501 tctccaagac ctatgccagc ggcttttccg cgctgaagac ggtcaacctt gaaatcaacc
     1561 gtggcgagat tttcgcgctg ctcgggccga acggcgcggg caagacgacg ctgatctcca
     1621 tcgtttgcgg catcgtcaac cgctcgtccg gcacggtcac ggtggatggc cacgacatcg
     1681 gcaccgacta tcgcgccgcg cgcggcctga tcgggctggt gccgcaggaa ctgaccattg
     1741 atgctttcga aaccgtctgg tcgacggtga atttcagccg tggcctgttc ggcaagcctg
     1801 ccaatccagc cttcgtcgag aaggtgctgc gcgacctgtc gctgtgggag aagaaggacg
     1861 ccaaggcgat cacgctttcg ggcggcatga agcgccggct gatgatcgcc aaggcgctgt
     1921 cgcacgagcc gcgggtgctg ttcctcgacg agccgaccgc cggcgtcgat gtcgaattgc
     1981 gccaggacat gtgggcgatg gtgcgcaggc tgcgcgagga tggcgtaacc atcattttga
     2041 ccacgcatta tatcgaggaa gcgcaggcga tggccgaccg tgtcggcgtc atcaaccagg
     2101 gagagattat cctggtcgag ggcaaggatg agctgatgca caagcttggc cgcaagcgac
     2161 tgatgctgga attgcgcaag ccgctcgaaa cggtgcccgc gtcgctcgcc cgttatgagc
     2221 tggaactgac ggcggatggc acgcaggcga cctacaccta tgacgaccag agcgaccggc
     2281 caggcgtcgc ctcgctgctg cgcgatttgg gcgcggaagg catcgagttc cgcgatctcg
     2341 acaccaaaaa cagttcgctg gaggaaattt tcgtcaatct ggtgaggaaa gacgcatgaa
     2401 tccgcgcgca atctgggcga tctaccggca ggaaatggcg cgcgccttcc gcacgctgtt
     2461 gcagagcatc gtgtcgccgg tcatctcgac ctcgctctat ttcgtcgtct tcggctcggc
     2521 catcggctcg cgcatcaccg aggtcgacgg catcagctat ggcgcgttca tcgtgccggg
     2581 cctgatcatg ctgtcgctgt tgacgcagtc gatctccaac gcctccttcg ccatctattt
     2641 cccgaaattc gtcggctcca tctatgagct gctttcggcg cccgtatcct atctggaaat
     2701 cgttatcgcc tatgtcggcg gggcggccac caagtccatt gtgctcggcc tcatcatcct
     2761 ggccaccgcc gcccttttcg tgccgctgca tatcgaacat ccgttctgga tgctcggctt
     2821 cctggtgctg acggcggtga cgttcagcct gttcggcttc atcatcggca tctgggccgg
     2881 cagcttcgag caactccagc tagtgccgct gctcatcgtc acgccgctca ccttcctcgg
     2941 cggcagcttc tattccatcg acatgctgcc gggcatctgg cgcacgatca cgctgttcaa
     3001 cccggtcgtc tacctcgtca gcgggttccg ctggagcttc tacggcaagg cggacgtttc
     3061 catcggcgtc agcctcggca tgacggtggt gttcctgctc gcctgcatcg caatcgtcag
     3121 ctggattttc aaaaccggct accggctcag gaactgacgc cgcacacctc cggcaaacgg
     3181 gcggcggcgg ctctggtggg ttttcgggtc ggcgcgctgc aacttggcgc tgcctgcggg
     3241 cttcatcagg ccacgatcgt ggacggggct tcgccacgac gcccgccttc acgcaaacgg
     3301 ccaagcgcga caggttgcgt gcctttagcc atttcggcaa gcggaacggc aacgcttgac
     3361 ccgctccttc ctatcggaaa caacggcata ttcgacgggc gtgaggggag cggcatgact
     3421 ttctggggtc tggttcagct tg
//
LOCUS       DASYCS010000317         4999 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2984536,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000317 DASYCS010000000
VERSION     DASYCS010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4999
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2984536"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..141
                     /locus_tag="VGM46_09940"
     tRNA            67..141
                     /locus_tag="VGM46_09940"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:100..102,aa:Glu,seq:ttc)
     gene            489..974
                     /locus_tag="VGM46_09945"
     CDS             489..974
                     /locus_tag="VGM46_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192953.1"
                     /translation="MSRTISSFALVAVLTGLLTALSFAVANHGYPFGAIGVKRLDAIA
                     DAGTFLPLAAVYFFSAVLMMVLPIRAAGVVLTNAADTIFWATVMLFAAIVGNLLARWA
                     FGQASALWALWNWRFLFVAAIVGCHLLANDFRRNVLLRSLFLLVFAGLTLACLFWTFT
                     V"
     gene            complement(990..1286)
                     /locus_tag="VGM46_09950"
     CDS             complement(990..1286)
                     /locus_tag="VGM46_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF982 domain-containing protein"
                     /protein_id="HEY4192954.1"
                     /translation="MHKQHFAIPVRVTGANGHSIEEIYSVDQALDFLQDWPNGRQGPL
                     YQAAFDACFGAGVDVVATEEAARAFMAFCRVSGIMARDMMTPNETDMTGRALPI"
     gene            1492..2319
                     /gene="map"
                     /locus_tag="VGM46_09955"
     CDS             1492..2319
                     /gene="map"
                     /locus_tag="VGM46_09955"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEY4192955.1"
                     /translation="MTTYVDATTAPLRNTGQIRLYDETGFAGMRKACNLTARCLDDLA
                     SLVKPGVTTNAIDRFVFEYGMDNGATPATLNYRGFTKSSCTSINHVVCHGIPDDKPLR
                     EGDIVNIDVTFILDGWHGDSSRMYPVGTVKRAAERLMEVTYECLMRGIAAIRPGSRTG
                     AIGAAIQTYAEAERCSVVRDFCGHGVGQLFHDAPNILHYGTASEGIEMRPGMIFTVEP
                     MINLGRPHVKVLSDGWTAVTRDRSLSAQYEHTVGVTDAGCEIFTLSPKGLDRPGLPA"
     gene            2368..3123
                     /gene="radC"
                     /locus_tag="VGM46_09960"
     CDS             2368..3123
                     /gene="radC"
                     /locus_tag="VGM46_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006335111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEY4192956.1"
                     /translation="MSKDGSDERGFFGETMLPPLQKPPSGEEKPHYLGHRDRLRERFS
                     LAGHIALADYELLELLLFRLIPRADTKPVAKALLARFGSLAEVLGAPETLLQEVKGIG
                     PAVALDLKIVAAAAQRMVRSELKGRETLSSWTQVLDYCRAAMAFEEREQFRILFLDKK
                     NALIADEVQQTGTVDHTPVYPREVIKRTLELSATAIVLVHNHPSGDPTPSRADIEMTK
                     EIVESGKRLGIAVHDHIIIGKKGHASMKGLLLI"
     gene            3317..4507
                     /locus_tag="VGM46_09965"
     CDS             3317..4507
                     /locus_tag="VGM46_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192957.1"
                     /translation="MTIHRSTFTKLAFSTILLTLPLNAAFAQDATAVADRLKASFARQ
                     NVDINWTGVTGDASSMVLQGVTAKAAAEKEAVGVGNVTLQGVTEADGGYSIETVSTEA
                     FTRKEGEAEISVTPIVIHDLTIPADGATGPTGALMLYKSAELENATVKVGGKTAFSVE
                     NIAVDITPPADGNAMEFSGKTGKFNADLTLIDDPKSKEVIDALGYQNITGSLQLAGSW
                     QPSDGKLEVSQYEIAVDNAGKLGMTFNLGGYTLDFIKSLQEMQKKMGEQPEGADKSAQ
                     GMAVLGLMQQLSFNSASIRYDDASLTNKVLDYVGKQQGMSGKDIANQAKAIVPFGLAQ
                     LNNPELTAQASAAIGKFLDDPKSLEISAVPPASVPFALIAAGAMANPLDLPKTLGVSV
                     KGNE"
     gene            4715..>4999
                     /locus_tag="VGM46_09970"
     CDS             4715..>4999
                     /locus_tag="VGM46_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002966073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEY4192958.1"
                     /translation="MRLTRQTNYAIRILMYCAANDGRLSRIPEIAAAYSVSELFLFKI
                     LQPLVEHGMVATVRGRNGGVKLGRSADSITLFDVVRVTEESFAMAECFESD"
BASE COUNT          987 a         1570 c         1445 g          997 t
ORIGIN      
        1 atcttgagcc cgagcaatgg gttgcccgtt ggcccggacg ccactataag ccgctttcat
       61 ctgagcgcgc ccatcgtcta gcggtcagga caccgccctt tcacggcggt aacaggggtt
      121 cgattcccct tgggcgtacc accttcatcc ggtccccggc atcctcaagc gcttcggcgg
      181 ttttgcacgt ccttcatcgg gattgctgga atgctttcga taagctatcc gggttcatcg
      241 aaaatcaggc ccgaggtatt ccgatgaggg accgtcacga tcatcatgac gagtatcttt
      301 ttaccagaga tcacaggacg ccggcgcagc aatcggctga caggcgtcag gcggctttcg
      361 gctggctgac cttgattgtc ttcacggcag cggtgttcgc cgccgcgcat ttcctcttgt
      421 gacctggcct tgccggcggt ggggctgagc agcttgccgc cttgcaaagc cctccaacag
      481 gaagcctcat gagcaggacc atctcttcat ttgcgctcgt ggccgtgctg acggggctgc
      541 tgaccgcgct cagcttcgct gtcgccaacc acggctaccc cttcggggcc atcggcgtaa
      601 aaaggctgga cgcaattgcc gatgccggca cgttcctgcc gctcgccgct gtctatttct
      661 tcagcgcggt gctgatgatg gtgctgccga tccgcgctgc cggcgtcgtt ttgaccaacg
      721 ccgccgacac gatattctgg gcaaccgtca tgttgttcgc cgccattgtc ggcaacttgc
      781 tggccagatg ggctttcggg caggcaagcg ccctttgggc gctctggaac tggcgcttcc
      841 tgttcgtcgc agcaattgtc ggatgccacc tgctggcaaa cgatttccgg cgcaacgtgc
      901 tgttgcgcag cctgttcctg cttgtcttcg ccgggctgac gctcgcctgc ctgttctgga
      961 ccttcacggt ctgagcggcg gcgccgacat cagatcggca gcgcccgccc cgtcatgtcg
     1021 gtttcgttcg gcgtcatcat gtccctggcc atgatgccgc tgacccggca gaacgccatg
     1081 aaagcgcgcg ccgcctcttc cgtcgcaacc acatcgacgc ccgcgccgaa acaggcatcg
     1141 aacgccgcct gatagagcgg accttgacgg ccgtttggcc aatcctgaag gaagtcgagg
     1201 gcctgatcga cgctgtagat ttcctcgatc gagtgcccgt ttgcacccgt tacgcgaacg
     1261 ggtatggcaa aatgctgctt gtgcatcgct agatttcgaa cggtcacctt tggagcggct
     1321 ccattcgtcc ttattgttat ccgggcgcat tcttgcaccc gattgtgtcg ccagtgcggt
     1381 gtctgccgcg acgggcaaac ggcgtgtgcg cgacttcgcc cttgaaccga acgcactcta
     1441 atgccaaatc aggggcaaag ttccgccagc ttcaaataaa gcaagacgaa tatgaccact
     1501 tatgtcgacg cgaccaccgc gccattgcgc aataccggcc agatccgcct ctatgacgag
     1561 acgggcttcg cgggcatgcg caaggcctgc aacctcaccg cgcgctgcct cgacgatctg
     1621 gcttcgctgg taaagccggg cgtcaccacc aacgccatcg accgtttcgt cttcgaatac
     1681 ggcatggata acggcgcaac accggcaacg ctgaactatc gcggtttcac caaatcctcc
     1741 tgcacgtcga tcaaccatgt ggtctgccac ggcattccag acgacaagcc gctgcgcgag
     1801 ggcgatatcg tcaacatcga cgtcaccttc attctcgatg gctggcatgg cgattcctcg
     1861 cgcatgtacc cggtcggcac cgtcaagcgc gcggcggaac gcctgatgga agtgacctat
     1921 gagtgcctga tgcgcggcat cgcggcgatc aggccgggca gccgcaccgg cgccatcggc
     1981 gccgccatcc agacttatgc cgaagccgag cgatgctcgg tggttcgcga tttctgcggc
     2041 catggcgttg gccagctttt ccacgatgcg cccaacatcc tgcactatgg cacagccagc
     2101 gaaggcatcg agatgcggcc gggaatgatc ttcaccgtcg agccgatgat caaccttgga
     2161 aggccgcatg ttaaagtgct gtcggacggc tggacggcgg tgacgcgcga caggtcgctg
     2221 tcggcgcaat atgagcacac cgtgggcgtg accgatgccg gctgcgagat tttcacgctt
     2281 tcgccaaaag gcctcgaccg gccgggcctg ccggcctagt atcgctacgg gccggcctta
     2341 ccggccaagg ggcggtacta gacagcgatg agtaaagacg gcagcgatga gcgcgggttt
     2401 ttcggcgaaa ccatgctgcc gccgttgcaa aagccgccat ccggtgaaga aaagccgcat
     2461 tatcttggcc accgcgaccg cctgcgcgag cgtttttcgc tggccgggca tattgccctt
     2521 gccgactatg aattgcttga actcctgctt ttccggctga taccaagggc cgacaccaag
     2581 ccggtggcga aggcgctgct ggcgcgtttc ggctcgttgg ccgaggttct gggcgcaccc
     2641 gaaaccctgc ttcaggaagt gaagggcatc ggccccgccg ttgctctcga cctgaagatc
     2701 gttgccgcgg cggcccagcg catggtccgc agcgaattga aagggcgcga gacgctgtcg
     2761 tcctggacac aggttctcga ctattgccgc gccgctatgg cctttgagga acgcgagcaa
     2821 ttccgcattc ttttcctcga caagaagaac gccctgatcg ccgacgaggt ccagcagacc
     2881 ggtacggtcg accacacgcc ggtctatccg cgcgaagtga taaagcgcac actggagctg
     2941 tcggcgaccg cgatcgtgct tgtccacaac cacccttccg gcgatccgac gccctcccgc
     3001 gccgacatag aaatgaccaa ggaaatcgtc gaaagcggca agcgcctggg catcgccgtg
     3061 cacgatcaca tcattatcgg caagaaaggc cacgcctcga tgaagggcct tctgcttatc
     3121 tagcagcgcg tttcctgccg cggtcgaagc agcaagtcgt tcaccaaaca acgaaaatga
     3181 cgtttgcccg attgcctttt gcctcgaatt ttgagaattg tgcccctccc cgccttcttc
     3241 cagtccactt tgtgaaacag aggaaggcag ccaaattcca caatgccggc ttgccaggtc
     3301 ccctcgggag aacgcgatga cgatccaccg ctctaccttc actaaacttg ccttttcgac
     3361 catccttctg acactcccgc tgaacgccgc cttcgcgcag gatgcgaccg ccgttgcaga
     3421 tcggctcaag gcttcctttg cccgccagaa cgtcgacatc aactggaccg gcgttaccgg
     3481 cgacgcatcc tccatggtgc tgcaaggcgt gacagccaaa gcggcggctg aaaaggaagc
     3541 ggtcggcgtc ggcaacgtca cgttgcaggg cgtgaccgaa gccgatggcg gctactcgat
     3601 cgaaacggtg tcgaccgaag cctttacccg caaggagggc gaggccgaaa tttccgtaac
     3661 cccgatcgtc atccatgact tgacgatacc ggcggacggc gccacgggac cgaccggcgc
     3721 actgatgctt tacaagtccg ccgaacttga aaacgccact gtcaaggtcg gtggcaagac
     3781 cgccttttcg gtcgaaaaca tcgcggtcga catcacgcct ccggccgacg gcaatgcgat
     3841 ggagttttcc ggcaagacgg ggaaattcaa cgccgacctg acgctgatcg acgaccccaa
     3901 gtccaaggaa gtcatcgacg cgctgggcta ccagaacatc accggctccc ttcaactggc
     3961 cggttcgtgg cagccttcgg acggcaagtt ggaagtgtcg cagtacgaaa tcgccgtcga
     4021 caatgccggc aagctcggca tgaccttcaa tctcggcggc tacacgctgg atttcatcaa
     4081 atcccttcag gaaatgcaaa agaagatggg tgagcagccc gagggagccg acaagtcggc
     4141 gcagggaatg gccgtgcttg gcctgatgca gcagctttcc ttcaacagcg ccagcattcg
     4201 ctatgacgat gcctcgttga ccaacaaggt gctggattat gtcggcaagc agcagggcat
     4261 gtccggcaag gatattgcca accaggcgaa agccatcgtg cctttcggcc tggctcagct
     4321 caacaacccg gaattgacgg cgcaggcctc cgctgccatc ggcaagttcc tcgatgatcc
     4381 gaagagcctg gaaatctcgg ctgtgccgcc tgcctcggtg cccttcgcgc tgatcgcggc
     4441 cggcgcgatg gccaatcccc tggacctgcc gaagacgctt ggcgtcagcg tcaagggcaa
     4501 cgaataatcc caaaggctgc aaagccggcg tgatcgaaca ggaaaatggc cgtgccgatg
     4561 cgcggccatt tttttgtcgc cgctgattcc agcggcaggc catatgcttc acagaatagt
     4621 tgacgagaaa aatcatctat ttaacgcgct tgtctggaaa tgttccaagg tatacgccat
     4681 attcattcca tcagtgcttc gctgaggaca aagaatgcgc cttacccgcc agaccaatta
     4741 cgccatccgg atcttgatgt actgcgctgc caatgatggc cggctcagcc gaatcccgga
     4801 aattgccgcc gcctactcgg tatccgagct tttcctgttc aagattttgc agccgctggt
     4861 cgagcacggc atggtggcga cggtgcgcgg gcgcaatggc ggcgtaaagc tggggcgcag
     4921 cgccgattcc attaccctgt tcgatgtcgt gcgggtgacg gaagagagtt tcgccatggc
     4981 ggaatgcttc gaatccgac
//
LOCUS       DASYCS010000318         2653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1536473,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000318 DASYCS010000000
VERSION     DASYCS010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1536473"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1355)
                     /locus_tag="VGM46_09975"
     CDS             complement(<1..1355)
                     /locus_tag="VGM46_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY4192959.1"
                     /translation="MKIRNIAIIAHVDHGKTTLVDQLLKQSGSFRENQRVAERAMDSN
                     DLEKERGITILAKATSVDWKDTRINIVDTPGHADFGGEVERILSMVDSAIVLVDAAEG
                     PMPQTKFVVGKALKVGLKPIVVINKIDRPDARHVEVVNEVFDLFAALDATDEQLDFPI
                     LYGSGRDGWVSENPEGPKDQELAPLFDLVLKHVPAPTVHPGPFRMIGTILEANPFLGR
                     IITGRIESGTLKANQPVKVLHHDGSLIETGRISKILAFRGLERQPIDEAQAGDIVAIA
                     GLSKGTVADTFCDPAVSEPLHAQPIDPPTVTMSFIVNDSPLAGTEGDKVTSRVIRDRL
                     LKEAEGNVALKIEESTEKDSFFVSGRGELQLAVLIETMRREGFELAVSRPRVVMQTDE
                     NGGLLEPVEEVVIDVDEEHAGVVVQKMSERKAEMVELRPSGGDRQRIVFHAPTRGLIG
                     Y"
     gene            1640..1921
                     /locus_tag="VGM46_09980"
     CDS             1640..1921
                     /locus_tag="VGM46_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192960.1"
                     /translation="MIRPAKSRLVLSLAGVAMAVALATGAQPAATRNAPLGDLDGAMT
                     DAQEMFSDAPDGVDPMVTGPVSASFKVKQADAGCGKAVWPNVPAGCYPN"
     gene            complement(2033..>2653)
                     /locus_tag="VGM46_09985"
     CDS             complement(2033..>2653)
                     /locus_tag="VGM46_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEY4192961.1"
                     /translation="SPQDHARVTAAIKAAEQKTNGEIYCVMAHASDGYFFPAALVSIV
                     GILLASLALSFWLEASWLSVRLPWFAAAQLLAAAAVLLLLWAAPRLRIHLVPHHLRFQ
                     AAHANAGKQFLAHNVHLTAARTGVLIFVSLAERYAEIVADSGIDSHVEQHVWDKIVQD
                     LTASAGDGRLVDGFVRAIEMAGVVLAEHFPVSPGDADELDNQLVEI"
BASE COUNT          460 a          791 c          885 g          517 t
ORIGIN      
        1 tgatagccga tgaggccgcg cgtcggcgca tggaagacga tgcgctggcg gtcgccgccg
       61 gaggggcgaa gctcgaccat ctcggccttg cgctcggaca ttttctgcac gacgacgccg
      121 gcatgctcct cgtcgacgtc gatgacgact tcctcgacgg gttcgagcaa gccgccgttt
      181 tcatccgtct gcatgacgac gcgcgggcgc gacacggcaa gctcgaagcc ttcgcggcgc
      241 atcgtttcga tcagcaccgc cagctgcaat tcgccacggc ccgacacgaa gaaactatcc
      301 ttctcggtcg attcctcgat cttgagcgcg acattgccct cggcttcctt gagcaggcgg
      361 tcgcggatga cgcggctggt caccttgtcg ccctcggtgc cggccagcgg gctgtcgttg
      421 acgatgaacg acatggtcac ggtcggcgga tcgatcggct gcgcatgcag cggctcgctt
      481 accgccggat cgcagaaagt gtcggcgacg gtgcccttgg aaaggccggc gatggcgacg
      541 atgtcgcccg cctgcgcctc gtcgatcggc tggcgctcaa ggccgcggaa ggcgaggatc
      601 ttggagatgc ggccggtttc gatcagcgag ccgtcatggt gcaggacctt gaccggctgg
      661 ttcgccttca gcgtgccgga ttcgatgcgg ccggtgatga tgcggccaag gaaggggttg
      721 gcttccagga tggtgccgat catgcggaac gggccggggt ggacggtcgg cgccggcaca
      781 tgcttcagca cgaggtcgaa cagcggcgcc aactcctggt ccttggggcc ttccgggttt
      841 tccgacaccc agccatcgcg gcccgaaccg tagaggatgg ggaaatcgag ctgctcgtcg
      901 gtggcgtcga gggctgcgaa caggtcgaac acctcgttga cgacttcgac atggcgcgcg
      961 tcggggcggt cgatcttgtt gatgacgacg atgggcttca gcccgacctt cagcgccttg
     1021 ccgacgacga acttggtctg cggcatcggg ccttcggcgg catcgaccag aacgatggcc
     1081 gaatcgacca tcgacaggat gcgctcgacc tcgccgccga agtcggcgtg gccgggggtg
     1141 tcgacgatgt tgatgcgggt gtccttccag tcgaccgagg tggccttggc aaggatggtg
     1201 atgccgcgct ccttttccag gtcgttggaa tccatggcgc gctcagccac gcgctggttc
     1261 tcgcggaacg agccggattg cttcaggagc tggtcgacca gggtggtttt tccatggtcg
     1321 acgtgcgcga tgatcgcgat attgcggatt ttcattttct ggtctcggga atgttgcggg
     1381 cagcaggccc cgcaatcgga ttgggacgct gcctttttca gttgcgcggc tcatacatgt
     1441 tttttcgcag atgcgaaagg gcatgttttt tcgcagtcgc cagaggacat gttttttcgc
     1501 agtcgccaaa gggcatgttt ttacgcggat acgaaaaggg caagcggcaa tcaaatggac
     1561 agtcaccaaa tcttaagggt attgggcgga gattattaac caatgcgggc gattcccgcc
     1621 tgccatgagt gcgtccagaa tgatccgtcc cgccaaatcc cgcctcgtgc tgtcgcttgc
     1681 cggcgtcgcc atggccgtgg cgctggccac cggcgcgcag ccggcggcga cgcgcaacgc
     1741 accgcttggc gacctcgacg gcgcgatgac ggatgcgcag gagatgtttt cggatgcgcc
     1801 cgatggcgtc gatccgatgg tgacgggtcc ggtcagcgcc tccttcaagg tgaagcaggc
     1861 ggatgcggga tgcgggaagg ccgtctggcc gaacgttccg gccggctgct acccgaactg
     1921 accggcggct agagcggttc tggttttacc gaatcggtaa aaccgctcta tctctttatt
     1981 tttggcggca tttccggacg caaaaccgtt tcacactttt gctggaaatg ctctagattt
     2041 ccaccaactg gttgtccaac tcgtcggcat cgcccggtga aaccgggaaa tgctcggcaa
     2101 ggaccacgcc cgccatttcg atcgccctga cgaaaccatc gaccaggcgt ccatcgccgg
     2161 cgctggctgt caaatcctgc acgatcttgt cccagacatg ctgttcgacg tggctgtcga
     2221 tgccgctgtc ggcgactatc tcggcgtagc gttcggccag cgacacgaaa atcagcacgc
     2281 cggttcgcgc cgccgtcaga tgcacattgt gggcgaggaa ctgcttgccg gcattggcgt
     2341 gcgcggcctg gaagcgcagg tggtgcggca ccagatggat gcgcagccga ggcgccgccc
     2401 acaacagaag caggaccgcg gcggcggcca gaagctgggc ggcggcgaac cagggcaggc
     2461 gtaccgacag ccatgaggct tccagccaga agctgagcgc caggctcgcc agcaggatgc
     2521 ccacgatcga aacaagggcg gcgggaaaga aatagccgtc gctggcatgc gccatgacgc
     2581 agtagatttc gccgttggtc ttctgttcgg cggccttgat ggctgccgtc acgcgggcgt
     2641 ggtcttgcgg gct
//
LOCUS       DASYCS010000319         5902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1244604,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000319 DASYCS010000000
VERSION     DASYCS010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 5902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5902
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1244604"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="VGM46_09990"
     CDS             <1..682
                     /locus_tag="VGM46_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY4192962.1"
                     /translation="PAYVLPDARMDHAIANLVDGAFYNSGQCCCGIERVYVHEKVYDA
                     FVEGFVAETKNYVVGNPLDQATTMGPMAQARFADLIREQKAEALRKGAKAHVGMKVSK
                     DEAGSPYLAPEVLTNVDHQMSVMREESFGPIVGIMKVRNDEEAIALMNDSPYGLTASI
                     WTSDTEHAVAIGDRVETGTVFMNRCDYLDPALVWTGVKETGKGASLSAIGYDNLTRPK
                     SYHLRETI"
     gene            865..2037
                     /locus_tag="VGM46_09995"
     CDS             865..2037
                     /locus_tag="VGM46_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEY4192963.1"
                     /translation="MTDLVSKWNYPTTVRFGAGRIRELPDALAATGIKHPLFVTDPGL
                     ARLPVVASTLKILDDAKVPYGVFSDVKANPVESNLIGGIAAFKKGKHDGVVAFGGGSA
                     LDLGKLIAFQAGQTRPGWDFEDVGDWWTRADADAIVPIVAVPTTAGTGSEVGRAGVIT
                     HEETHTKKVIFHPKMLPEIVIADPELSVGMPSFITAGTGMDALAHCLEAYCAPGYHPM
                     ADGIGVEGVRLVFENLPKVFADGGDLAARAHMMSAAAMGATAFQKGLGAIHSLSHPIG
                     ALYDTHHGMTNAVFMPYVLAFNREAIEEKIERLAAYCGIKGGFDGFAAAVMDLRKRLN
                     VPHTLPEFIKGLDMDAARRGLVADMAIVDPTAGGNPIQLTREGALSLLDAAMSGTI"
     gene            2291..3397
                     /locus_tag="VGM46_10000"
     CDS             2291..3397
                     /locus_tag="VGM46_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006206235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY4192964.1"
                     /translation="MFRFTGKILSLTAVALMATSAISSADSMDDLVAAAKAEGQLTTI
                     ALPHDWCGYGAVIDAFKAKYPEITVNELNPDAGSGDEVEAIKANKDNKGPQAPDVIDV
                     GLSFGPSAKAENLLMPYKVSTWDSIPDSAKDADGYWYGDYYGVLAMMVNKDLVKEAPK
                     DWADLLKPEFANAVALAGDPRASNQAIQAVYAAGLSTGAAGGEAAGTAGLDFFKKLNA
                     AGNFVPVIGKPATLAQGQTPILINWDYNTLSGRDTLNGNPPVDVIVPATGVVAGVYVQ
                     AISAYAPHPNAAKLWMEYLYSDEGQVGWLKGYCHPIRFNDLAKNGKVPQEMLDKMPPA
                     AAYEKAVFPSLEEQGAAKDAITKNWDSVVGANVK"
     gene            3488..4402
                     /locus_tag="VGM46_10005"
     CDS             3488..4402
                     /locus_tag="VGM46_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011976265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY4192965.1"
                     /translation="MTNLTLSDPAVAENMAPPAEARRMRFPTQWVGVAPFFIFTLMFL
                     VLPTLYLVLGAFQNDAGEYTLENIFALSQPSIVAAYWISIKISVTSSLIGALAGLAIA
                     IAIVRGGLPGWIRSATLTFSGVASNFAGVPLAFAFLATLGRLGLATVILNTVFGLNIY
                     AHGFNILSFWGLTLTYVYFQIPLMVVIITPAIDGLKREWGEAAATLGATQAQYWRMVV
                     IPVIWPSFLGTVVLLFANSFGAIATAYALTGSSLNIVPILLYAQIRGDVLHNPHLGYA
                     IAFGMIFITGLANILYIWFRTRSERWLK"
     gene            4399..5190
                     /locus_tag="VGM46_10010"
     CDS             4399..5190
                     /locus_tag="VGM46_10010"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4192966.1"
                     /translation="MKSGKFWAWIVFALGAAYFFIPLIGTLEFSMRMRRGVYSFDAYK
                     VVLGDARFQATFGYSVLAAIFTIILGVLIVVPTAYWIRLRLPRLRPVVEFITLLPLVI
                     PAIVIVFGYIRMYGSNSPLPFLASARGTDALLVIGYATLALPYMYRAVDTGLRTIDVA
                     TLTEAAQILGAGWATIITRVILPNVLIAVLSGAFLTFAIVIGEFTMASLLNRPAFGPY
                     LQNMGANRAYEPAALAIIAFAITWGCMSLIQVLSRFAPKSATRPN"
     gene            5202..>5902
                     /locus_tag="VGM46_10015"
     CDS             5202..>5902
                     /locus_tag="VGM46_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006206232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4192967.1"
                     /translation="MAEPFLSIQHVNKSYGSNTVVQDFNLDVQAGEFVSFLGPSGCGK
                     TTVLRMVAGFEEPSAGKIVVTGKDITNLKPNQRNVGMVFQAYALFPNLTVAQNIGFGL
                     KVAGVARADIDKRVTEMLDLIKLPQMADRYPYQLSGGQQQRIALGRAIAPKPKLLLLD
                     EPLSALDAKVRVSLREEIRAIQKELGITTIFVTHDQEEALSISDRIAVMYGGKAEQVG
                     TPFEIYNRPTTKFVA"
BASE COUNT         1128 a         1809 c         1781 g         1184 t
ORIGIN      
        1 cccggcctat gtgctgcccg acgccaggat ggaccatgcc atcgccaatc tggtcgacgg
       61 cgcgttttat aattccggcc agtgctgctg tggcatcgag cgcgtctatg tgcatgagaa
      121 ggtctatgac gcgttcgtcg agggcttcgt tgccgagacg aagaactatg tcgtcggtaa
      181 cccgctcgac caggcgacca cgatggggcc gatggcgcag gcgcgctttg ccgacctgat
      241 ccgtgagcag aaggcggagg cgctacgcaa gggggcgaag gcgcatgtcg gcatgaaggt
      301 cagcaaggac gaggccggtt cgccctacct tgcgccggaa gtgctgacaa atgtcgacca
      361 ccagatgagc gtgatgcgcg aggaaagctt cggtcccatc gtcggcatca tgaaggtgcg
      421 caacgacgag gaagccatcg cgctgatgaa cgacagcccc tacgggctga ccgcttcgat
      481 ctggacaagc gataccgagc atgcggtggc gatcggcgac cgggttgaaa ccggcaccgt
      541 gttcatgaac cgctgcgact atctcgatcc ggcgctggtg tggaccgggg tgaaggaaac
      601 cggcaagggc gcgtcgctgt cggccatcgg ctacgacaat ttgacgaggc cgaagagcta
      661 tcacctcagg gaaacgatct agtcccgttc aatgcaatcg accctctgcg cctcgcttcg
      721 cccggcactt ctcgccgaaa gggtgaggcg ccaccgccgc gatgcgggag atttttcagc
      781 ttgggttcct cgcccctttt ttgggagagg taactggcag ggcagaagag gggttcttga
      841 aaccatggaa agccagcaaa cgtcatgacc gatcttgtct ccaaatggaa ctatcctacc
      901 accgtgcgtt tcggcgcggg gcgcatccgc gaattgccgg atgcgctggc ggcaacgggc
      961 atcaagcatc cgctgttcgt caccgatccg ggcctggcca ggctgccggt ggtcgcctcg
     1021 acactgaaga tactcgacga cgccaaggtg ccctatggcg tcttttccga cgtgaaagcg
     1081 aacccggtcg aatccaacct gatcggcggc atcgccgcct tcaaaaaggg aaagcatgac
     1141 ggcgtcgtcg ccttcggcgg cggttcggcg ctggatctcg gcaagctgat cgctttccag
     1201 gccgggcaga cgcggccggg gtgggatttc gaggatgtcg gcgactggtg gacgcgcgcc
     1261 gatgccgacg ccatcgtgcc gatcgtcgcc gtgccgacga cggcgggaac gggttcggaa
     1321 gtcggccgcg ccggcgtcat cacgcatgag gagacgcata ccaagaaggt tatcttccat
     1381 ccgaagatgc tgccggaaat cgtcatcgcc gatcctgaat tgtcggtggg catgccaagc
     1441 ttcatcaccg ctggcaccgg catggacgcg ctggcccatt gcctcgaagc ctattgcgcg
     1501 cccggctacc atccgatggc ggacggcatc ggcgtcgaag gcgttcggct ggtgttcgaa
     1561 aatctgccga aagtgtttgc tgatggcggc gatctcgctg cccgcgcgca catgatgtcg
     1621 gcggcggcga tgggcgcgac cgccttccag aagggcctcg gtgcgatcca ttcgctgtcg
     1681 cacccgatcg gcgcgctcta cgacacccat cacggcatga ccaacgccgt gttcatgccc
     1741 tatgtgctgg cgttcaaccg cgaagccatc gaggaaaaga tcgaacggct cgccgcctat
     1801 tgcggcatca agggcggttt cgacggcttt gcggccgccg tcatggattt gcgcaagagg
     1861 ctgaatgtcc cgcatacgct gccggaattc ataaaggggc tggatatgga cgccgcgcgc
     1921 aggggcctgg tcgccgatat ggcgattgtc gatccgacag ccgggggaaa cccgatccag
     1981 ttgacgcggg aaggtgcttt gtcgcttttg gacgcggcta tgtcgggaac gatatagaag
     2041 ctgcgcgaaa cagttgcgct tttctttcga gaagatacaa aatgaaaaaa aataacaggg
     2101 aatgcaaaca gggagggcac cgggactttt ttagccggga aataaatggg cgccgattgc
     2161 aggcacccat aaaaaacagt taatttttta cgccttcagg atgcggagca gccggatttc
     2221 atccgactgt cacataacgt ggataccgca tcgcaggcgt ccaacggcgt cagttcaacg
     2281 gagagaacca atgttcagat ttacagggaa aattctttcc cttacggccg tcgcgctcat
     2341 ggcgacgtca gccatttcgt ccgcggattc gatggatgac ctcgtggccg ccgccaaggc
     2401 ggaaggccag cttacgacaa tcgcgctgcc gcatgactgg tgcggctacg gcgccgtcat
     2461 cgacgcgttc aaggcaaaat atcccgagat caccgtcaac gaactcaacc ccgacgccgg
     2521 ttcgggcgac gaggtcgagg cgatcaaggc caacaaggac aataaggggc cgcaggcgcc
     2581 cgacgtgatc gacgtcggcc tttcctttgg cccgtccgcc aaggccgaaa accttttgat
     2641 gccttacaag gtatcgacct gggattcgat ccccgacagc gccaaggatg ccgacggcta
     2701 ctggtacggc gactattacg gcgtgctggc gatgatggtg aacaaggacc tcgtcaagga
     2761 agccccgaag gactgggccg atcttttgaa gccggaattc gccaatgcgg ttgcgcttgc
     2821 gggcgatccc cgcgcctcga accaggcgat ccaggcggtc tatgccgccg gcctctcgac
     2881 cggcgccgcc ggtggtgaag ccgccggtac tgccggcctc gacttcttca agaagctgaa
     2941 cgccgccggc aatttcgttc cggtcatcgg caagccggcg acgcttgcgc agggccagac
     3001 gccgatcctg atcaactggg actacaacac gctttcgggc cgcgacacgc tgaacggcaa
     3061 tccgccggtc gacgtcatcg ttccggccac gggcgttgtt gccggcgtct acgtgcaggc
     3121 gatcagcgcc tacgcgccgc acccgaacgc cgccaagctg tggatggaat atctttattc
     3181 cgacgaaggc caggttggct ggctgaaggg ctattgccac ccgatccgct tcaacgacct
     3241 cgccaagaac ggcaaggttc cgcaggagat gctggacaag atgcctccag cggctgccta
     3301 cgaaaaggcc gttttcccct cgcttgaaga gcagggcgcg gccaaggacg ccatcaccaa
     3361 gaattgggat agcgtcgtcg gcgccaacgt caagtaaacc gaaatcttcc gggcggccaa
     3421 agattgcttc ggccgcccgg attcgttgta atgtctctca acagccttgg aagacggtag
     3481 ctggcgaatg accaatttaa cgctttccga tccggccgtt gccgaaaaca tggcgccgcc
     3541 ggccgaggcg cgccgcatgc ggttcccgac ccaatgggtg ggtgtggcgc ctttcttcat
     3601 attcacgctg atgttcctgg ttttgccgac gctctatctg gtgctgggcg cattccagaa
     3661 cgatgccggt gaatatacgc tggaaaacat ctttgccctt tcgcagccat ccatcgtcgc
     3721 cgcctactgg atttccatca agatcagcgt gacctcctcg ctgatcggcg cgctcgccgg
     3781 cctcgccatc gctatcgcca tcgtacgcgg cggcctgccc ggctggatac gctcggcaac
     3841 gctgacattt tccggcgttg cctccaattt cgccggcgtg ccgctggcct tcgcctttct
     3901 ggcgacgctg ggcaggcttg gcctggccac ggtgatcctg aacacggttt tcgggctgaa
     3961 catctacgcg catggcttca acatcctgtc gttctggggg ttgacgctta cctatgttta
     4021 tttccagatt ccgttgatgg tggtcatcat cacgccggct atcgacggcc tcaagcggga
     4081 gtggggcgaa gccgccgcca cgctgggggc aacgcaggcg caatattggc gcatggtggt
     4141 catcccggtc atctggccga gctttctcgg cacggtcgtg ctgcttttcg ccaattcctt
     4201 cggcgccatc gcgacagcct atgcgctgac gggatcgtcg ctcaatatcg tgccgatcct
     4261 gctttatgcg cagatccgtg gcgacgtgct gcataatccg catctcggct atgccatcgc
     4321 cttcggcatg atcttcatca ccggtctcgc caatattctc tacatctggt tccggacacg
     4381 ttcggaaagg tggctcaaat gaagtctggt aaattctggg cctggatcgt ctttgcgctc
     4441 ggcgcggcct atttcttcat tccgctgatc ggcacgctcg aattctcgat gcgcatgcgg
     4501 cgcggcgtct attccttcga cgcctacaag gtggtgcttg gcgatgcgcg cttccaggcg
     4561 acgttcggct attcggtgct ggcggcgatc ttcaccatca tccttggcgt acttatcgtc
     4621 gtgccaacgg cctactggat aaggctgcgg ctgccgcggc tgcgcccggt cgtcgagttc
     4681 atcacgctgt tgccgctggt catcccggcc atcgtcatcg tcttcggtta tatccgcatg
     4741 tatggatcga attcgccgct gcctttcctg gcaagcgcgc ggggcaccga cgcgctgctg
     4801 gtgatcggct atgcgacgct ggccttgcct tacatgtacc gggcggtcga tacaggcctg
     4861 cgcaccatcg acgtggcgac gctgacggaa gcagcgcaga tactcggcgc cggctgggcg
     4921 acaatcatca cccgcgtcat cttgccaaac gtgctgatcg cggtgctgtc gggggcgttc
     4981 cttaccttcg ccatcgtcat cggagaattc accatggcca gcctgctcaa caggccggcc
     5041 ttcggtccct atctgcaaaa tatgggcgcc aatcgcgctt atgaaccggc agcgcttgcc
     5101 atcatcgcct tcgccatcac ctggggctgt atgtcgctca tccaggtcct gtcgcgcttc
     5161 gcgccgaaat cggcaacccg cccgaactga aagtcgaaac catggccgaa ccgttccttt
     5221 caatccagca tgtcaacaaa tcctacggtt ccaataccgt ggtgcaggac ttcaacctcg
     5281 atgtgcaggc cggcgaattc gtttccttcc tcgggccatc gggctgcggc aagacgacgg
     5341 tgctgcgcat ggttgccggc ttcgaggagc cgagcgccgg caaaatcgtc gtcaccggca
     5401 aggatatcac caatctgaag cccaaccagc gcaatgtcgg catggtgttc caggcctatg
     5461 ccctgtttcc caatctgacg gtggcgcaga atatcggctt cggcctcaag gtcgccggcg
     5521 tggcgcgcgc cgacatcgac aagcgcgtga ccgagatgct cgacctcatc aagctgccgc
     5581 agatggccga ccgttatccc tatcaattgt ccggcggcca gcagcagcgc atcgcgcttg
     5641 gccgcgccat tgcaccgaag cccaaattgc tgttgcttga cgagccgctt tcggcgctcg
     5701 atgccaaggt tcgcgtatcg ctgcgcgagg aaatccgcgc gatccagaaa gagcttggca
     5761 tcaccaccat cttcgtcacg cacgaccagg aagaggcgct ttcgatttcg gatcgcatcg
     5821 cggtgatgta tggcggcaag gccgagcagg tcggcacgcc gttcgagatt tataaccgtc
     5881 cgaccaccaa gttcgtcgcc aa
//
LOCUS       DASYCS010000320         2250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_522146,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000320 DASYCS010000000
VERSION     DASYCS010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522146"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1346)
                     /gene="ppk1"
                     /locus_tag="VGM46_10020"
     CDS             complement(<1..1346)
                     /gene="ppk1"
                     /locus_tag="VGM46_10020"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEY4192968.1"
                     /translation="MNELKPVQTEFAAVEATIAEGGNPDRFVNREFSWLQFNRRVLEE
                     SLNIHHPLLERVRFLSISAANLDEFFMVRVAGLAGQVREGISLRSPDGRTPQQQLDQL
                     LHEVERLQEDQQKSLSGLVELLQKEGIESITREGLTKDEKIWLEDYFQEQVFPVLTPL
                     SIDPAHPFPFIPNLGFSMALQLRNRKNGEAMSALLRLPVALRRFIRLPDRKNHVRFIP
                     LEDAVSLYIGKLFPGYEVKGAGTFRLIRDSDIEVEEESEDLVRLFETALKRRRRGQVI
                     RIEFDSQMPEELREFVATELGVVANRVSVLTGPLALNQISEMVSVARDDLKFVPYNPR
                     FPERIREHGGDCFAAIREKDIVVHHPYESFDVVVQFLRQAMADPEVVAIKQTLYRTSN
                     DSPIVRALVDAAEAGKSVTALVELKARFDEEANIRWARDLERAGVQVVFGFIELKT"
     gene            1514..1759
                     /locus_tag="VGM46_10025"
     CDS             1514..1759
                     /locus_tag="VGM46_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011975282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEY4192969.1"
                     /translation="MAGGSIPHFQNDAGHPAIDIGVREFMCVGANAPFDHPHVFLDMG
                     DDDEKVCPYCSTLYRYSPALKATETLPAGCLYIDRAA"
     gene            1778..>2250
                     /locus_tag="VGM46_10030"
     CDS             1778..>2250
                     /locus_tag="VGM46_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006200490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY4192970.1"
                     /translation="MDDTLSRQVVIAGAGIGGLSAALAFAERGYTVRLYEQASHIEAA
                     GAGIQLSPNATHVLKRLGVLDRLLPSAVRPQAVVLKDASTLAELASVPLGEDGQRRWK
                     APYLVAHRGDLQNAMLAEIAGIPEIELVTGARLSTLATHSRGITASFLVEGNLKQA"
BASE COUNT          435 a          708 c          662 g          445 t
ORIGIN      
        1 tgggttttca gctcgatgaa gccgaaaacc acctgcacgc cggcccgttc caggtcgcgc
       61 gcccagcgaa tgttcgcttc ctcgtcgaag cgcgccttaa gctccaccag cgccgtcacc
      121 gatttgccgg cttccgccgc atcgaccagc gcgcgcacga tcgggctgtc gttggatgtg
      181 cggtaaagcg tctgcttgat ggccaccact tccgggtcgg ccatggcctg gcgcaggaac
      241 tgcaccacta cgtcgaagga ttcgtaaggg tggtggacga cgatatcctt ctcgcggata
      301 gcggcaaaac aatcgccgcc atgttcgcgg atacgctcgg ggaaacgcgg attgtagggc
      361 acgaatttca aatcgtcgcg cgccaccgat accatttccg agatctggtt cagggccagc
      421 ggcccggtaa gcacgctgac acggttggcg acaacgccaa gctcggtggc gacgaactcg
      481 cgcagttcct ccggcatctg gctgtcgaac tcgatgcgga tcacctggcc gcgccggcgc
      541 cgtttcagcg ccgtctcgaa caggcgaacg aggtcttccg attcttcctc gacctcgata
      601 tcgctgtcgc ggataagccg gaacgtgccg gcgcccttga cctcatagcc ggggaaaagc
      661 ttgccgatgt aaaggcttac cgcgtcttcc agcgggatga agcgaacatg gttcttgcgg
      721 tccggcaggc ggatgaaacg gcgcagcgcc acaggcagac gcagtagcgc gctcatcgcc
      781 tccccgttct tgcggttgcg aagctgcaag gccatcgaga agccgaggtt tggaatgaac
      841 ggaaacggat gcgccggatc gatcgaaagc ggcgtcagca ccggaaagac ctgttcctgg
      901 aaatagtctt ccagccagat cttctcgtcc ttggtcaggc cttcgcgggt aatgctttcg
      961 atgccctcct tttgcagaag ctccaccagc cctgaaaggc tcttctgctg atcttcctga
     1021 agcctttcga cctcgtgcag caactggtcc aactgctgct gcggcgtgcg cccatccgga
     1081 cttctgagcg aaatgccctc ccgaacctgg ccggccagcc cggcaacgcg aaccatgaag
     1141 aattcatcga gattggccgc cgaaatcgac aggaagcgca cgcgctccag cagcggatgg
     1201 tggatgttca acgattcttc caggacgcgg cggttgaatt gcagccagga aaactcacgg
     1261 ttgacgaacc tgtcagggtt gcctccctcg gcaatcgtcg cctcgacggc ggcaaattct
     1321 gtttggaccg gcttcaactc gttcattttc gatttgccca tgttccctct ggatcgccgg
     1381 caaggttgtg acctagtcgg ctcaacttat tctgtggcag ggttttacga cagtttcatt
     1441 tcatcgaact tgcaaaaatc tgtcttatgc tccaaacgct gcaagtccga ccgaactgag
     1501 acggagacga aaaatggctg gcggttccat tccccatttc cagaacgatg ccggccatcc
     1561 ggccatcgac atcggcgtca gggaattcat gtgcgtcggc gccaacgcgc ccttcgacca
     1621 tccgcatgtt ttcctcgaca tgggcgacga cgacgaaaaa gtctgtccct attgctcgac
     1681 gctctaccgc tattcgcccg cattgaaggc aaccgaaacg ctgccggcgg gctgccttta
     1741 catcgaccgc gctgcctgac cgcgccgcgc cgcgcgcatg gatgatacgc tgtcgcggca
     1801 ggtcgtcatc gccggggcag gcataggcgg gttgagcgcc gcactggcct ttgccgagcg
     1861 cggttatacc gtgcggctat atgaacaagc ttcccatatc gaggcagccg gcgcgggcat
     1921 ccagctttcg ccgaacgcca cgcacgttct caagcggctg ggcgttctcg accggctttt
     1981 gccatcggcg gtgcggccgc aggccgtcgt cttgaaggat gccagcactt tggcggaact
     2041 ggccagcgtt ccgctgggag aagacggcca aaggcgctgg aaggccccct atctcgtcgc
     2101 ccatagaggc gacctgcaaa acgccatgct ggcggaaatt gccggcatac ccgaaatcga
     2161 gcttgtcacc ggcgcgcggc tgagcacgct ggcgacgcat tcgcgcggca tcacggcgtc
     2221 gtttctcgtc gagggcaacc tcaagcaggc
//
LOCUS       DASYCS010000321         2655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1827648,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000321 DASYCS010000000
VERSION     DASYCS010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1827648"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..120
                     /locus_tag="VGM46_10035"
     CDS             <1..120
                     /locus_tag="VGM46_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020012481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3008 family protein"
                     /protein_id="HEY4192971.1"
                     /translation="TKKSELKGASKGMYESMSEKQLEEFAETKRKGLPAKKSD"
     gene            complement(239..448)
                     /locus_tag="VGM46_10040"
     CDS             complement(239..448)
                     /locus_tag="VGM46_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006206016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192972.1"
                     /translation="MKKFFLALTGAAMLAGSAMAVPQTAEAAGWHHNRPHQVCRVVVK
                     KRVVWRNHRRHVERYRVRQCNWVRR"
     gene            758..1375
                     /gene="rpsD"
                     /locus_tag="VGM46_10045"
     CDS             758..1375
                     /gene="rpsD"
                     /locus_tag="VGM46_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011179373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEY4192973.1"
                     /translation="MSKRESAKYKIDRRLGENIWGRPKSPVNKREYGPGQHGQRRKGK
                     LSDFGLQLRAKQKLKGHYGDMSEKQFRKAYDEANRRKGDTSENLIGLLESRLDAVVYR
                     AKFVPTIFAARQFVNHGHVNVNGKRTNIGSYRCKPGDVVEVREKSKQLTIVLEAVGLA
                     ERDVPDYIEADHNKMTATFSRIPVLSDVPFAVQMEPNLVVEFYSR"
     gene            1522..2382
                     /gene="ttcA"
                     /locus_tag="VGM46_10050"
     CDS             1522..2382
                     /gene="ttcA"
                     /locus_tag="VGM46_10050"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018324706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEY4192974.1"
                     /translation="MTELATAGAPPEEAAESGYHPLFNDVPSSVEFNKLRKRLLRQTR
                     QSIEDFAMVKPGERWLVALSGGKDSYGLLALLLDLKWRGLLPVELLACNLDQGQPNFP
                     KHILPDYLDSNGIAHRIEYRDTYSIVTEKLPQSSTYCSLCSRLRRGHLYRIAREEGCS
                     ALVLGHHREDILETFFMNLFHGGRLAAMPPKLLNDEGDIMVLRPLSYCAEADLAKFAA
                     AMKFPIIPCDLCGSQDGLQRNIMKAMLDDIEKKMPGRKDTMIHALANARPSHLLDRKL
                     FDFAGLSAAG"
     gene            complement(2383..>2655)
                     /locus_tag="VGM46_10055"
     CDS             complement(2383..>2655)
                     /locus_tag="VGM46_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4192975.1"
                     /translation="ALDMLLFGLLGSNFNALAMEPLGHVAGTASSVLGFTQTFGGGIL
                     GTIIGQMFNGTVTPLAAGFCCVSVAAFVMVVIAERGKLFQPHNPAV"
BASE COUNT          558 a          815 c          789 g          493 t
ORIGIN      
        1 accaagaaaa gcgaactcaa gggtgcatcc aaggggatgt acgaatcgat gagcgaaaaa
       61 cagttggaag aattcgccga gaccaagcgc aagggcctgc cggccaagaa gtccgactag
      121 gcaagtgcat tccaaatctg cccggcaagc ggcggcggaa gcgtttgcat ttatggacat
      181 gcatatgaaa acggcggctg aacccagccg ccgttttcat tgaaagaagt gaggccgatc
      241 agcgacggac ccaattgcac tggcgcacgc ggtagcgctc gacatgtcgg cgatggttgc
      301 gccagacgac ccgcttcttg acgacgacgc ggcaaacctg atgagggcgg ttgtggtgcc
      361 agccggcggc ctcggcggtc tgcggtactg ccatggccga tccagccagc atcgctgcgc
      421 ccgtcaacgc gagaaaaaac ttcttcatgg atgatctcct gctatttggc ctcatgttcg
      481 gccggtggta gtcccgtccg acgtcaatta acggtcatcc agatgaatag ttgctgaatg
      541 gcgccgtcac aaacttgaca tcggcacggc tgtcggtcta tccacccgtc aacaccgggc
      601 ggcgacgctc ggtgctttct tttgacgtgt cccgtgggtt ttccgccgct ttgcgtagga
      661 aaccctgtca ggcttcgaaa acggaggcca gaggagggcg cgtttccttc acccggacca
      721 tgaggttccg ggtggttcgt tttgaaaagg gaatgcgatg agcaagcgcg aatccgcgaa
      781 atacaagatc gaccgccgtc tcggcgaaaa catctggggc cgcccgaaat ccccggtcaa
      841 caagcgtgaa tacggtcccg gccagcacgg ccagcgccgc aagggcaagc tttccgattt
      901 cggtctccag ctgcgcgcca agcagaagct gaagggccac tatggcgata tgtccgaaaa
      961 gcagttccgc aaggcctatg acgaagccaa ccgccgcaag ggcgacactt cggaaaacct
     1021 gattggcctg ctcgagtcgc gcctggatgc ggttgtctac cgcgccaagt tcgtgccgac
     1081 gattttcgcc gcgcgccagt tcgtcaacca tggccatgtc aacgtcaacg gcaagcgcac
     1141 caacatcggc tcctaccgtt gcaagccggg cgatgtcgtc gaggttcgcg agaagtcaaa
     1201 gcagctgacg atcgttctcg aagccgtcgg cctcgccgag cgcgacgtac cggattatat
     1261 cgaagccgat cacaacaaga tgaccgcgac cttctcgcgc attccggtgc tgtcggacgt
     1321 gcccttcgcg gtgcagatgg aaccgaacct ggtcgtcgaa ttctattcgc gctgatcttc
     1381 gatccacgat ccagctcgaa aaggccgccc ttcgaggcgg ccttttcttt atgcggccgg
     1441 tccggtaaat ccatccggca gaacgttcag gacttttcac cgatgaatat gctgtcagag
     1501 ccgcttgtgg ccgaactgcc gatgaccgaa ctggccacag ccggcgcgcc gcccgaggaa
     1561 gcggctgaga gtggctatca tccgctcttc aacgacgttc catcctccgt cgagttcaac
     1621 aagctgcgca agcggctgct gcgccagaca cgccagtcga tcgaggattt cgccatggtc
     1681 aagccgggcg agcgctggct ggtcgcgctg tcgggcggca aggattccta cggcctgctg
     1741 gcgctgttgc tggatctcaa atggcgcggc ctgttgccgg tcgagctgct ggcctgcaac
     1801 ctcgaccagg gacagccgaa tttcccgaaa cacatcctgc ctgattatct cgactcgaac
     1861 gggatcgccc accgcatcga atatcgggac acttattcca tcgtcaccga gaagctgccg
     1921 caaagcagca cctattgctc gctttgctca cggctgcggc gcggtcatct ctatcgcatc
     1981 gcgcgcgagg aggggtgctc ggcgctggtg cttggccacc accgtgaaga catactcgaa
     2041 acctttttca tgaacctgtt tcacggcggc cggctggcgg cgatgccgcc gaagctgctc
     2101 aatgacgagg gcgacatcat ggtactgcgc ccgctcagtt attgcgctga ggccgacctg
     2161 gccaagttcg ccgccgcaat gaagtttccg atcatcccgt gcgacctttg cggtagccag
     2221 gacggcctcc agcgcaacat catgaaggcg atgctggacg acatcgaaaa gaagatgccg
     2281 ggccgcaagg acaccatgat ccacgcgctg gccaatgcgc ggccttcgca tctgctcgac
     2341 cgcaaactgt tcgatttcgc cggattatcc gccgccggct gattacaccg ccggattatg
     2401 cggctggaac agcttgccgc gctcggcgat caccaccatc acgaaggcgg cgaccgacac
     2461 gcagcagaag ccggcggcca gcggcgttac cgtgccgttg aacatctgcc cgatgatggt
     2521 gcccaaaatg ccgccgccga aggtttgcgt gaagcccagc accgatgagg cggtgccggc
     2581 gacatggccg agcggttcca tggccagcgc gttgaagttc gagccgagaa ggccgaacaa
     2641 aagcatgtcc agagc
//
LOCUS       DASYCS010000322         5402 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_87513, whole
            genome shotgun sequence.
ACCESSION   DASYCS010000322 DASYCS010000000
VERSION     DASYCS010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 5402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5402
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87513"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..335)
                     /locus_tag="VGM46_10060"
     CDS             complement(63..335)
                     /locus_tag="VGM46_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010025739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEY4192976.1"
                     /translation="MNVLWTPIGWDDYTYWQAVDPKIVIRINELIKDARRTPFSGIGK
                     PEPLRGEAAGWWSRRITQEHRLVYRVVGKGDFQALEIAACRFHYSR"
     gene            complement(332..586)
                     /locus_tag="VGM46_10065"
     CDS             complement(332..586)
                     /locus_tag="VGM46_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEY4192977.1"
                     /translation="MGYVTLTELRANMAKHFDKVEQDRIELVVTRQNHEPVVVMPLAE
                     LEGLRETLHLLSSPANAERLRESMAQANAGELEEHELIEP"
     gene            685..1668
                     /gene="ubiA"
                     /locus_tag="VGM46_10070"
     CDS             685..1668
                     /gene="ubiA"
                     /locus_tag="VGM46_10070"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEY4192978.1"
                     /translation="METIQSKAARGRVADAPSGHWVYRVLPRSVWPYAQLARWDRPIG
                     WQLLLWPCWWSVALAASAYARPGDPLLTLLPSPLTLILFLIGAVAMRGAGCTYNDLVD
                     EGIDSQVERTRSRPLPSGQVSRRQAWAFLFAQALAGLIVLLQFDSFAVGLGISSLGIV
                     AVYPFAKRVTNWPQLVLGLAFSWGALMGWANEFGDIDGPAVMLYIGSILWTIGYDTIY
                     AHQDKEDDAIVGVRSTARLFGENTKAWLAGLYGGALMCMAIAFAAAQAPVVALAGLIA
                     AGAHMARQIAVLDIDNPDQCLKLFKSNNQVGWLIFAGLVGGALWVALKPLV"
     gene            complement(1715..2281)
                     /locus_tag="VGM46_10075"
     CDS             complement(1715..2281)
                     /locus_tag="VGM46_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6101 family protein"
                     /protein_id="HEY4192979.1"
                     /translation="MNTGLKPVWAGRNMRLDPFRLPQVVSYATSDDYGDVTFTIDKRG
                     AVVRRMLEMSGLPATIALPAMAFRGVAARAMEDPDGNVTVTLELLHCDPMLSVPLLVA
                     DDLDDVAADWRAWADAYRLPMLLIEADGVARTLEESLGAAIKTMPVQERRKGRVSTMR
                     RPRFLARRRTGDLGLRLVIDNQEIISRQ"
     gene            complement(2466..3896)
                     /locus_tag="VGM46_10080"
     CDS             complement(2466..3896)
                     /locus_tag="VGM46_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY4192980.1"
                     /translation="MTSNVQNLDAALIARFAAIVGDSHALQDRQDIAGYLIEPRGLWH
                     GKTPLVLRPGSVEEVGRIMKLATETGTPIVPQGGNTGLVGGQMPDGTGRDVVLSLSRL
                     NRIREIDVLSNTATVEAGVILQNLHAAADAVDRLFPLSLASQGSCEIGGNLSSNAGGT
                     GVLAYGNARELCLGVEVVLPTGEVFDDLRKLKKDNTGYDLKNLFVGAEGTLGVVTAAV
                     LKLFPKPRGREVAFVGLASAQAALDLFSLAMDRGGGNLTAFELMERRPYDFTLRHAPG
                     IVRPLAEDWPWYVLMEISSGRSAEDATGLVEDILSTGLEAGTVGDAVISASVGQANAF
                     WNFREMLPEAQKPEGASIKHDISVPIASIPAFIEQAAKAVEAVSPGARPICFGHMGDG
                     NLHYNVSRPLGGDDQAFLTLYHPMNKAVHDLVRAFKGSISAEHGIGQLKRDELIATAP
                     PMAIDLMRRVKAAFDPAGIMNPGKVI"
     gene            complement(3923..4897)
                     /locus_tag="VGM46_10085"
     CDS             complement(3923..4897)
                     /locus_tag="VGM46_10085"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008839293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEY4192981.1"
                     /translation="MAEILLADKAMERALALLEAGDVVAIPTETVYGLAGDATNGEAV
                     AGIFEAKGRPRFNPLIAHVSDMAMAERIGLFDPLSRKLAEKFWPGPLTLVVPVRADAG
                     IHPLVTAGLDTIALRMPKGFGAALIGRFGRPLAAPSANASGKISPTSAAAVAASLGGR
                     IKLVVDGGATPVGLESTIIKVEGENVRLLRPGGIAADEIEAVLGAGLHRGGAGGIQAP
                     GMMASHYAPGAAVRLAARTVDKGEALLAFGPLRADGWEGAAAVKNLSEAGDLRMAASN
                     LFSYLQELDRSGAAIIAVEPIPMTGLGEAINDRLARAAAPRNGFFAEN"
     gene            5059..>5402
                     /locus_tag="VGM46_10090"
     CDS             5059..>5402
                     /locus_tag="VGM46_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4192982.1"
                     /translation="MRVIGAFILTIALTTGAARASSFIVIDEAAPASTPSIVQLGQPA
                     PAKMAKGQAPSSWIDRSLGQLQQAFAAMKQPDMRRFPSLEAIPSGEAISADNMATPSI
                     IAMGEPAPGIGY"
BASE COUNT          964 a         1761 c         1678 g          999 t
ORIGIN      
        1 ctattgggcc gggcgcgtga ggtcaagcca ttacgcatgg ccgatacagg cttgcgccat
       61 gcctagcggc tatagtgaaa ccggcaggcg gcgatttcca gcgcctgaaa atcgcccttg
      121 ccgacaacgc gataaacgag ccgatgctcc tgggtaatgc gccttgacca ccagcctgcc
      181 gcctcaccgc gcaggggttc cggcttgcct atgcccgaga aaggggtgcg gcgcgcatcc
      241 ttgatgagtt cgttgatacg aatgacaatc ttggggtcta cggcctgcca atatgtataa
      301 tcatcccagc cgatcggagt ccaaagaacg ttcatggctc aattaattcg tgctcttcaa
      361 gttcgccagc atttgcctga gccatcgact cgcgcagcct ctcggcattg gcgggcgaac
      421 tcaaaagatg aagcgtttcg cgaaggccct ccagttcggc cagcggcatg accaccaccg
      481 gttcatgatt ctgcctggtc acgaccagct cgatcctgtc ctgctccacc ttgtcgaaat
      541 gcttggccat gttggcccgc agttctgtca atgtcacata gcccatggtt tgcctccaat
      601 acgcatcttg cgtacatata cacgtacgct tcgggatgag caagaacgct tgacgcgaag
      661 cgtcggcgct gccaatccac aacgatggaa accatccagt cgaaagccgc gcgcgggcgc
      721 gttgccgatg cgccgagtgg ccattgggtc tatcgggtgc tgccgcgctc agtgtggccc
      781 tatgcgcaac ttgcgcgctg ggaccggcca atcggctggc aactgctttt gtggccgtgc
      841 tggtggtcgg tggcgctggc ggccagcgcc tatgcgcggc cgggcgatcc gctgctgacc
      901 ttgctgccct cgccgctgac cctcattttg ttcctgatcg gcgcggttgc gatgcggggc
      961 gccggctgca cctataatga tcttgtcgac gagggcatcg acagccaggt ggagcgcacc
     1021 cgttcgcgcc cgctgccttc gggccaggtc agccgccggc aggcgtgggc gttcctgttt
     1081 gcgcaggcgc tggcggggct gatcgtgctt ctccagttcg acagcttcgc ggtcggcctc
     1141 ggcatttcgt cgctgggcat cgtcgccgtc tacccgttcg caaaaagagt caccaactgg
     1201 ccgcaactgg ttctcggcct tgccttttcc tggggcgcgc tgatggggtg ggccaacgag
     1261 ttcggcgaca tcgacggccc ggccgtcatg ctctatatcg gctcgatcct gtggacgatc
     1321 ggctacgaca cgatctacgc gcaccaggac aaggaagacg acgccattgt cggcgtgcgt
     1381 tccaccgcgc gcctgttcgg ggaaaacacc aaggcatggc tggccggcct ttatggcggc
     1441 gcgctgatgt gcatggccat tgcctttgcc gcggcgcagg cgccggtcgt cgccctcgcc
     1501 gggttgatcg cggccggcgc ccacatggcg cggcagatcg cggttctgga tatcgacaac
     1561 ccggaccagt gcctgaaact gttcaaatcg aacaaccagg tcggctggct gatctttgcc
     1621 gggcttgtcg gcggcgcgct atgggtagcg ctgaagccgc tggtgtgaga ggcgtacccg
     1681 cccttggcca tggcctgcgt gtgtatgcag gccgttactg gcgggaaatg atttcctgat
     1741 tgtcgatgac cagcctcaag ccaaggtcgc cggtgcggcg gcgggcaagg aaccgtgggc
     1801 ggcgcatggt ggaaacccgc cccttgcggc gctcctggac aggcatcgtc ttgatcgccg
     1861 cgcccagcga ttcttccagc gtgcgagcga cgccgtccgc ctcgatcaga agcatcggca
     1921 ggcggtaggc atcggcccag gcgcgccaat cggcggccac gtcatccaga tcgtcggcca
     1981 ccagcagcgg caccgacagc atcggatcgc aatgcagcag ttcaagcgtg acggtgacat
     2041 tgccgtccgg atcttccatg gcgcgcgcgg caacgccgcg aaaagccatg gcgggaagcg
     2101 cgatggtggc cggcagcccg ctcatctcaa gcatgcggcg cacgaccgca ccgcgcttgt
     2161 cgatggtgaa ggtcacgtcg ccataatcgt cgctggtcgc gtagctgacc acttgcggca
     2221 ggcgaaatgg gtcaagacgc atgttgcgcc ccgcccagac tggcttcagt ccggtgttca
     2281 tcgattgtct ttccctgtct ctcctgagag ccggttttcc ggtctctccg ggccagtttt
     2341 cccggcctcg tttatgaggg gagactagcg cggggtcctt accgccgcgc ttaagaaagt
     2401 cggttaaatt ttgctgatct tgcagatggt tatcggattc ccaccgaaaa accgaaagga
     2461 aagaatcaga taaccttacc gggattcatg atgcccgccg gatcgaacgc cgccttcacc
     2521 ctgcgcatca ggtcgatggc catcggcggc gccgtggcga tcaattcgtc gcgcttcaac
     2581 tggccgatgc catgttcggc cgagatcgac cctttgaagg cgcgcaccag atcatgcacc
     2641 gccttgttca tcggatgata gagagtgaga aaggcctggt cgtcgccacc gagcggccgg
     2701 gaaacattat agtgcaaatt gccgtcgccc atatggccga agcagatggg ccgggcgccg
     2761 gggctgaccg cttcgacggc ctttgccgcc tgctcgataa acgccgggat agaagctatc
     2821 ggcactgaaa tgtcgtgctt gatcgaggcg ccttcgggct tttgcgcctc aggcagcatt
     2881 tcgcggaagt tccagaaggc attggcctgt ccgacgctgg cggaaatcac cgcatcgccg
     2941 accgttccgg cttcaaggcc ggtggagaga atgtcctcga ccagcccggt tgcatcctcg
     3001 gcggagcggc ccgacgatat ttccatcagc acataccacg gccaatcctc cgccagcggg
     3061 cgaacgatgc ccggcgcatg acgaagggta aagtcgtagg gccgccgttc catcagctcg
     3121 aaggcggtca gattgccgcc gccgcgatcc atggccagcg aaaacaggtc cagcgccgcc
     3181 tgcgcggagg cgaggccgac gaaagccact tcgcgccccc gcggcttggg gaaaagcttc
     3241 agtaccgctg ccgtgacgac gccaagcgtg ccttcagcgc cgacgaacag gttcttcagg
     3301 tcgtagccgg tattgtcctt cttcagcttg cgcagatcgt cgaacacctc gcccgtcggc
     3361 agcaccactt cgacgccgag gcaaagctcg cgcgcgttgc cataggccag cacgccggtg
     3421 ccgccggcat tggaggaaag attgccgccg atctcacacg aaccctgcga cgccagcgaa
     3481 agggggaaaa gcctgtccac ggcatcggcg gcggcatgca gattttgcag gatgacgccg
     3541 gcttcgacgg ttgcggtgtt cgacagcaca tcgatctcgc ggatgcggtt cagccgcgac
     3601 agcgacagca ccacgtcccg gccggtgcca tccggcatct ggccgccgac aagcccggta
     3661 ttgccaccct gcggcacgat cggcgttccg gtttcggtgg ccagcttcat gatccggcca
     3721 acctcctcga cgctgcccgg acgcagcacc agcggcgtct tgccgtgcca caggccgcgc
     3781 ggctcgatca gatagccggc gatgtcctgc cggtcttgca aggcgtggct gtcgccgacg
     3841 attgccgcaa agcgggcgat gagggcggca tcgagatttt gtacattcga agtcatttga
     3901 acatccgaac aagaggtaat ggctagtttt cggcaaaaaa tccgttgcgc ggggcggcgg
     3961 cgcgcgccag ccggtcgttg atggcctcgc caagccccgt catcggtata ggctcgaccg
     4021 cgatgatggc cgcgccgctt cgatccagtt cctgaaggta ggagaaaagg ttgctcgccg
     4081 ccatgcgcaa atcgccggcc tcggagaggt tcttcaccgc cgccgctcct tcccagccgt
     4141 cggcgcgcaa cgggccgaac gccagcagcg cctcgccttt gtccactgtc cgcgcagcca
     4201 ggcgcacggc ggcccctggc gcgtaatgcg aagccatcat gcccggcgcc tggatgccgc
     4261 cggccccgcc cctgtgcagg ccggcgccga gaaccgcttc gatttcatcg gcggcgatgc
     4321 cgcctggccg cagcagccgc acgttttcac cctcgacctt gatgatcgtc gattccagcc
     4381 cgaccggcgt cgcgccgcca tcgaccacca gcttgatccg cccgcccagc gaagcggcga
     4441 cggcggccgc actggtcggg cttatcttgc cggatgcgtt ggcgctgggc gcggcgagcg
     4501 gccggccgaa ccggccgatc aacgccgcgc caaagccctt gggcatacgc agcgcaatgg
     4561 tatccagccc cgccgtgacc aatggatgga tgccggcatc ggcgcgcacc ggcaccacca
     4621 gcgtcagcgg tccgggccag aatttctccg ccagtttccg cgacagcgga tcgaacaggc
     4681 cgatccgctc ggccatggcc atgtcgctga catgagcgat cagcggattg aagcggggcc
     4741 gccccttggc ttcgaagatg ccggccaccg cctctccatt ggtcgcatcg cccgccaggc
     4801 cgtagaccgt ctcggtgggt atggcgacga cgtcgccggc ttcaagcaga gccagcgccc
     4861 gttccatcgc tttatcggcg agcagtattt cagccaaaac cgcaaccctt cctctgccgg
     4921 ttgctataac gccgggaccc gccccgcaag cgcgcgttgg tgaattataa atccctaaag
     4981 tgctatccat tcgcgtaaac cgccgtcaat gccgacaagg tacgatgcgg ccaaaaggca
     5041 atggagcctg ttttatccgt gcgtgtaatt ggagcgttca tcctgacgat cgcgctgacg
     5101 acgggcgcag cccgcgcgtc atccttcatc gtcattgatg aggccgcgcc ggcgtcgacg
     5161 ccttccatcg tccagcttgg ccagccggcc ccggcaaaga tggccaaggg ccaggcgccc
     5221 tcctcgtgga tcgacagatc gctcggccag ttgcaacagg cattcgcggc catgaagcaa
     5281 ccggatatgc gccgttttcc atcgctggaa gcgataccat ccggggaagc gatctcggcc
     5341 gacaacatgg caacgccgtc gatcatcgcc atgggcgaac ccgcgccggg gataggatac
     5401 ga
//
LOCUS       DASYCS010000323        11774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3277268,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000323 DASYCS010000000
VERSION     DASYCS010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 11774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11774
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3277268"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1328
                     /locus_tag="VGM46_10095"
     CDS             <1..1328
                     /locus_tag="VGM46_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEY4192983.1"
                     /translation="TVTLAGTPDLALNFAGPVPFAFLAKKLAAQGLSLTGTANVNVRV
                     NGPASKPVIAGTIAATGARFIDAGSGLAVNDIALDVAIANGVARINRLTGTLSTKGSL
                     TVGGTVGIDAAQGFPADLTVKLSDGRYTDGRVVTANLGGDLTVKGPLASAPIIAGTVN
                     LARTVITVPDKLPGSLTALGVKRKNAPAAVVAQDKALKPASAGGGGGGGLALDINIKA
                     PSQIFIQGRGVDAELGGTLRLTGPTSSPQAVGAFKLRRGRLEILGKRLNFTDGTLTFS
                     GSLVPYLNLTAQSTASDATVTIVVSGEATNPKFTFSSVPALPEDEVLARLIFGRSMSN
                     LSPLQIAQLAEAAGQLAGVGGSTSLLQNLRSAIGVDDLDVTTDEKGGTAVSAGKYLND
                     RTYLSLQKGEKPGSGKARIDLDVGRGVKLRGEASDGGEAKGGVFYEREY"
     gene            1603..3258
                     /locus_tag="VGM46_10100"
     CDS             1603..3258
                     /locus_tag="VGM46_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006205860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY4192984.1"
                     /translation="MTFYKSNGDRVPEVVEKLAKSAKAGEMDRREFLAIASAMGASTA
                     MAYGMIGLAAPTQAHAAETPVKGGTIRCSMSIKAQKDPRSYDWSELANATRTFLEPLV
                     KYTTSYTFEPMLLESWDVNDDATEYTLHVRKGATWNNGDEFNADDVMFNLLRWCEKAE
                     GNSMQARMGGLVDSKTNKAAEGAITKVDEFTIKLKLPASDISIIPNFADYPGLVLHRD
                     FEKNGADIVKNPVGTGAFELVSYDVGSKVVAKRRENGKWWGGEAYLDGIEFIDYGTDS
                     SASASAFESGEIDTIYQTMSEFVEILDGLGLVKSEIKTATTITCRANIGHKPYDDQKV
                     RNALQMAVDNATVLQLGYNNYGFVGENHHVCVIHPEYYELPKKSRDIEGAKKLMAEAG
                     QMDFEHELITVDEEWHKNTGDAIAAQLREAGFKVKRTVLPGSTFWNDWTKYPYSTTNW
                     NMRPLGVQVLSLGYTTGASWNESAYANPEFDKKLNEALAIADPEKRKEIMKDIEQILQ
                     DSGVIIQPFWLSLFNHSSKKVKDNPMHPTLEVNYEKVWLDPNA"
     gene            3457..4491
                     /locus_tag="VGM46_10105"
     CDS             3457..4491
                     /locus_tag="VGM46_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019859185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4192985.1"
                     /translation="MISFIARRLATMVFTMICLTLVVFYLVNLEPNLKKLALAQTNSR
                     ATQEEVSSWLVKNGYRENFVKRYAQWIGAWPKQPAVDADGKPVQRFHYCPEALTPKFS
                     GVLQGDFGCSTKFRTTVAAKLFPALGATGILMFFVLLVMVPVSLLIGILAGMREGSRT
                     DRVLSVASIASTATPEYVSGVIFTVIFASWLGWFNGSAASATFSGITFYNFTLPVITL
                     AIYGIGYIARMTRASMVEVMTQQYIRTARLKGLNFKSVVIKHALRNALIAPFTVIMLQ
                     FPWLLTGVVIVEVMFRYQGFGYTLVEAAGNNDIELLLGCSLVSVFIVLITQLISDVGY
                     AYLNPRIRVQ"
     gene            4503..5660
                     /locus_tag="VGM46_10110"
     CDS             4503..5660
                     /locus_tag="VGM46_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006336363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4192986.1"
                     /translation="MQMNYIDGFQVVLGVLIRFWPVWVALVIVMGASIVSKHKLGLYG
                     QLFDSYVGIFGVGLCLFWLFTAIFASTIAPFDPLGQFPIMKDTLPGAIEPQSGLVYLF
                     GGDKLARDVFSRMVYGSQIVLIIAPAATGFALMVGITLGLPAGYYGGKIDTVLSFLAN
                     LVLAFPVILLFYLLVTPGIMDTPIPYAMAGLFFLFPIVFFCVLFWTRFKNRPDRIYIL
                     MGLTLILGGWIYMGLVFNADPLGLISMAPNQLNIFVAVVFASSPGVFRIVRGLVMDIK
                     TRDYVAAAQTRGETPWYIMLWEILPNARGPLIVDACLRIGYTTILLGTLGYFGLGLAP
                     ESPDWGTAIKDASRLLRQFIHPALPPTVALMSFVLGLNLLADALREQSMKD"
     gene            5680..8133
                     /locus_tag="VGM46_10115"
     CDS             5680..8133
                     /locus_tag="VGM46_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019859183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4192987.1"
                     /translation="MNEAVKAANTANAEPIIEIENLSISFFTRKGEIPAVMDFSCSVM
                     AGEAMGIVGESGCGKSTVSLGIMRDLSNIGKIVGGKIKFQGKDMGELTEEELRAIRGN
                     KIAMIYQEPMASLNPAMKVGQQLMEVPLIHDKVSKEEAYKRSLEMVRAVKLPDPERMM
                     RSFPHQLSGGQQQRIVIAMALLSKPALLLLDEPTTALDVTVEAGIVDLVRGLGEKFGT
                     SMIFVSHNLGLILETCDRITVMYSGEAVETGKIKDVFDRMRHPYTQGLFRSIPLPGAD
                     KNSRPLIAIPGQLPLPHERPKGCNFGPRCHHFVEGVCDAAEIPMIPVEGHDGHYSRCV
                     RFNEIDWDALPPGAQKVTEKVEPGAPVLEIADLRKYYKVGGNEVFGGSDGRVVKANET
                     ISFTARESETVAIVGESGCGKSTLAKVLLGLETASSGTVTLDGKEIQSTGVESRNVDT
                     VSSIQMVFQNPFDTLNPSHSVGSQIIRTLEKFRVGKNPGERRQRMLELLDLVKLPRAF
                     ETRKPRQLSGGQKQRIGVARAFAGDAKVVVADEPVSALDVSVQAAVTELLMDIQRKNK
                     TTMLFISHDLSVVRYIADRVVVMYLGYIVEQGTTDQIFAPPYHPYTEALLSAIPIADT
                     SVVKKHIVLEGDIPSAMNPPSGCPFQTRCGYKHLVPGNKCETLVPPVKDLGDGHLSLC
                     WLDDDVLAMMEPVISFDKADKAPVVDATPEDGDSDDRPADEAASYDAPKRPRGKKASP
                     EASAFGAAAEEAESTARARRHEVRDETSETGGASTSGNSTLSPFTERTVGDGRQAVDE
                     NVAPASKPARKPKAAPKKK"
     gene            complement(8149..8739)
                     /locus_tag="VGM46_10120"
     CDS             complement(8149..8739)
                     /locus_tag="VGM46_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEY4192988.1"
                     /translation="MYASRFSKIVPVIALLALSACATAPRHINNICSVFDQRDGWFNN
                     WQSAAKRAEAKYGVPVPVLMATVRKESGFKANARPPRTKLLGFIPWKRPSSATGFSQA
                     LDGTWAQYKRESGNFAARRTSFADAVDFVGWYHEKTADTLGVAKNDTYNLYLAYYSGW
                     VAYQRGSWKSNASLQRYARDTDEMARNYAVQLRECD"
     gene            8952..10490
                     /locus_tag="VGM46_10125"
     CDS             8952..10490
                     /locus_tag="VGM46_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEY4192989.1"
                     /translation="MSEPRRKRGAERTSNRGPAAIPQLPLRRVVNPYPPMAVLSADQI
                     EAIHEASMHILENFGIEVMSPRALSLFEKAGAEVDHASMNVRIDRGLVAQALKTTPSA
                     YALTPRNPAHAVHLGGNSINFTLVAGPPNVHDMERGRRSGNMRDYGDLIRLAQHFNCI
                     HMLGNQVCAPIELPANSRHLDTYFANLTLTDKSFHVSAIGQGRALDGIEMMAISRGLS
                     LDQMRDDPGLTTIISVNSPRRFDDMMSDGLMTMAEHGQSVAVTPFTLMGAMSPVTLAG
                     ALAQQNAEALFGVVLTQLVRPGAPVMYGAFTSNVDMKSGAPAFGTPENTKANIASGQL
                     ARRYNLPYRTTPGSASNAADAQGAYETSMALWGAILGHGNLVYHAAGWQEGGLTASFE
                     KFIIDVEMIQNMMEVLRPIEVNEGELALEALGAVPTGGHFFGEPHTLERYSTAFYQPM
                     LSNWQNYEAWEEAGGLDATARATRLWKKALEEYVEPTMDIAVREELEAYVAKRKEAIG
                     QGEP"
     gene            complement(10553..11326)
                     /locus_tag="VGM46_10130"
     CDS             complement(10553..11326)
                     /locus_tag="VGM46_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013894600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY4192990.1"
                     /translation="MPKTPSFFWYELMTSDMDAAEAFYTDVVGWTAQPFPGAAGMPRY
                     TVMNAGERGVGGVMNLPDAAKEMGMPPAWLGYIYSSDADASAEAVKKAGGKVHREPAD
                     IPDVGRFAVVADPQGAMFMLLQPNGPDMPPVAAGTPGHVGWHELYTSDWQAAMDFYSG
                     QFGWTKDHPFDMGEMGTYQIYAVDGAQSGGMMNKPPQVPVPVWQFYFNVPAIDAAVQR
                     VNDKGGKVLMGPMEVPGGQWVVNCTDPQGAHFALVAPVR"
     gene            11569..>11774
                     /locus_tag="VGM46_10135"
     CDS             11569..>11774
                     /locus_tag="VGM46_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidine dehydrogenase"
                     /protein_id="HEY4192991.1"
                     /translation="MALSLFKEGIEGGRLSPEAYAENFSDLHPPLDHHEALVESDRCY
                     FCYDAPCMNACPTSIDIPLFIRQIS"
BASE COUNT         2284 a         3569 c         3666 g         2255 t
ORIGIN      
        1 gcacggtgac gcttgccggc acgccggatc tggcgctgaa tttcgcgggg ccggttccgt
       61 ttgcctttct tgccaagaag ctggccgcgc aagggctttc actgaccggc accgccaacg
      121 tcaatgtgcg ggtgaacggg ccggcaagca agccggtcat cgccggtact attgcggcaa
      181 cgggcgcgcg cttcatcgat gccggttccg gccttgccgt caatgacatc gcgctcgatg
      241 tcgccatcgc caacggtgtc gcgcgcatca accggcttac cggaacttta tcgaccaagg
      301 gcagcctgac ggtgggcggc actgtcggca tcgatgcggc gcaaggcttt ccagccgacc
      361 tgacggtcaa gctgagcgac gggcgctaca cggacgggcg cgtcgtcacc gccaatctcg
      421 gcggcgacct gacggtcaag gggccgctcg cttcggcgcc gatcattgcg ggaaccgtca
      481 accttgcccg caccgtcatt accgtgccgg acaagctgcc gggatcgctg acggcgcttg
      541 gtgtcaagcg caagaacgcg cctgccgccg tggtggccca ggacaaggcg ttgaagccgg
      601 catcggcggg cggcggcggg ggcggcgggc tggcgctcga cataaacatc aaggcgccca
      661 gccaaatatt catccagggc aggggcgtcg acgccgagct gggcggcacg ctgcgattga
      721 ccggaccgac ctcgtcgccg caagccgtcg gcgcattcaa attgcggcgg gggcggctgg
      781 aaatcctcgg caagcggctg aacttcaccg atggcacgct gaccttctcc ggttcgctcg
      841 tgccctatct caacctgacg gcgcaatcga ccgccagcga cgcgacggtg acaatcgtcg
      901 tgtcgggcga agcgaccaat ccgaaattca ccttctcgtc ggtgccggcc ttgccggagg
      961 atgaagtgct ggcgcggctg atcttcggcc ggtcgatgtc gaacctgtcg cctttgcaga
     1021 tcgcgcagct ggccgaggcg gcgggccagc tggccggcgt gggcggctcg acctcgctgc
     1081 tgcaaaatct gcgcagcgcc atcggcgtcg acgatctcga cgtgaccacg gacgaaaaag
     1141 gcggcaccgc cgtttccgcc ggcaagtacc tcaacgaccg cacttacctg tcgttgcaga
     1201 agggcgagaa accggggtcc ggcaaggcaa ggatcgatct cgatgtcggg cgcggggtca
     1261 agctgcgtgg cgaagcctcg gatggcggcg aagccaaggg cggcgttttc tacgagcgcg
     1321 aatactgacg gccaatcggc ccgcgatttt gaattggcac tcgggcgcac gaaagacgta
     1381 tgccgcaacg taaacttctg gattaactca tttcaaaccg gccagtaatt caaccggatt
     1441 tgcccgcgct ttaacagatt tgcgtcagag ttgtcgcaat tgcgcgaacc gcaccccgat
     1501 caaagttgca cattcttggc gagttccgga ttcagggctt tttgacatct cggcccggat
     1561 gcggaatgtt aatggcgaaa acgccgaaaa tgggagttag tcatgacatt ctacaagagt
     1621 aacggcgacc gcgtgccaga ggtcgtcgag aagctcgcaa agagcgccaa ggcaggcgag
     1681 atggaccgcc gcgaattcct cgccatcgca agcgcgatgg gcgcttcgac cgcgatggct
     1741 tatggcatga tcggccttgc cgcgccgacg caggcgcatg ccgccgagac gccggtgaag
     1801 ggcgggacga tccgctgctc gatgtcgatc aaggcgcaga aagacccgcg cagctacgac
     1861 tggtcggaac ttgccaacgc cacgcgcacc ttcctggagc cgctggtcaa gtacaccacc
     1921 tcctatacgt tcgagccgat gctgctcgaa agctgggacg tgaacgacga cgccaccgaa
     1981 tataccttgc atgtgcgcaa gggcgcgacg tggaacaatg gcgatgaatt caacgccgac
     2041 gacgtgatgt tcaaccttct tcgctggtgc gaaaaggccg agggcaattc gatgcaggca
     2101 cgcatgggcg gcctcgtcga ttccaagacc aacaaggccg ccgaaggcgc catcaccaag
     2161 gtcgatgaat tcaccatcaa gctgaagctg cctgcttcgg atatctcgat catcccgaat
     2221 ttcgccgatt atcccggcct cgtgctgcac cgcgatttcg agaagaacgg cgccgatatt
     2281 gtcaagaatc cggtcggcac cggcgcgttt gaactggttt cctacgacgt tggctcgaaa
     2341 gtcgtggcaa agcgccgcga aaacggcaaa tggtggggcg gcgaagccta tctcgacggt
     2401 atcgagttca tcgactacgg caccgattca tcggcctcgg ccagcgcgtt cgaatccggc
     2461 gagatcgaca cgatctacca gaccatgtcg gaattcgttg aaattctgga tgggcttggt
     2521 ctcgtcaagt cggagatcaa gacggcaacc accatcacct gccgcgccaa tatcggccac
     2581 aagccctatg acgaccagaa ggtgcgcaac gcgctgcaaa tggccgtcga caacgccacc
     2641 gtgcttcagc tcggctacaa taattacggc ttcgtcggcg agaatcacca cgtctgcgtg
     2701 attcacccgg aatattacga acttcccaag aagagccgtg acatcgaggg cgcgaagaag
     2761 ctgatggcgg aagccggcca gatggacttc gagcatgagc tgatcaccgt cgacgaggaa
     2821 tggcacaaga acaccggcga cgcgatcgcg gcgcagctgc gtgaggccgg cttcaaggtc
     2881 aagcgcacgg ttctgcccgg ctcgaccttc tggaacgact ggaccaaata tccctactcg
     2941 accaccaact ggaacatgcg tccgctgggc gtccaggtgc tgtcgctcgg ctacaccacc
     3001 ggcgcgtcct ggaacgagtc ggcctatgcc aatcccgagt tcgacaagaa gctgaacgag
     3061 gccctggcga tcgccgatcc ggagaagcgc aaggaaatca tgaaggatat cgagcagata
     3121 ttgcaggatt ccggcgtcat catccagccg ttctggcttt cgctgttcaa ccactcgtcg
     3181 aagaaggtga aggacaaccc gatgcacccg acgctggaag tgaactacga aaaagtatgg
     3241 cttgatccaa acgcctgatt ttcaggccaa aaagagggga aatccacgcg gtttcccctc
     3301 aaacgggaac atttctcaag catcagcccc gaacgccggt ttccggtttc ggatcatttg
     3361 atgccctgtc gaagcagcat cgtgtccttt acgtgcgtcc tgtcgggcac atggtgcgtc
     3421 agcgtttcaa accccaagcc tcgaggaggg gcctaggtga tatcgttcat agcccggcgt
     3481 ctggccacca tggtgttcac catgatctgc ctgacgctcg tcgttttcta tctcgtcaat
     3541 ctggagccga acctcaagaa actcgcgctg gcgcagacga acagccgtgc gacgcaggag
     3601 gaggtttcca gctggctggt caagaatggc taccgcgaga atttcgtaaa gcgctatgcg
     3661 cagtggatcg gcgcctggcc caagcagccc gccgtcgacg ccgacggcaa gccggtccag
     3721 cgcttccatt actgtcccga ggcgctgacg ccgaagtttt cgggcgtttt gcagggcgat
     3781 ttcggctgct cgaccaagtt ccgcaccacg gttgccgcca agctgttccc ggcgcttggc
     3841 gcgaccggca tcctgatgtt cttcgtgctt ttggtgatgg tgcctgtctc gctgttgatc
     3901 ggcatcctgg cgggaatgcg tgaaggttcg cgcaccgacc gcgtgctgtc ggtggcctct
     3961 atcgcctcga cggcgacgcc cgaatatgtc tccggcgtca tcttcaccgt cattttcgca
     4021 agctggctcg gctggttcaa cggctcggcc gccagcgcca ccttcagcgg catcaccttc
     4081 tacaatttca cgctgccggt catcacgctg gccatttacg gcatcggcta catcgcgcgc
     4141 atgacgcggg cttcgatggt cgaggtgatg acgcagcaat atatccgcac cgcgcggctg
     4201 aaagggctga acttcaagag cgtcgtcatc aagcatgcgc tgcgcaatgc gctgatcgca
     4261 ccgttcaccg tcatcatgct gcaatttccc tggctgctga ccggcgtcgt catcgtcgag
     4321 gtgatgttcc ggtatcaggg cttcggctac acgctggtgg aagcggcggg aaacaacgac
     4381 atcgagcttc tgctcggctg ctcgctggtt tcggtgttca tcgtcttgat cacgcagctc
     4441 atttccgatg tcggttacgc gtatctcaat ccgcgcatcc gggttcagta gggggacggc
     4501 cgatgcagat gaactacatc gacggcttcc aggtggtgct tggcgtactg atccgcttct
     4561 ggcccgtatg ggtggcgctg gtcatcgtga tgggcgccag catcgtctcg aagcacaagc
     4621 ttggcctgta cgggcagctt ttcgacagct atgtcggcat tttcggcgta ggcctctgcc
     4681 tgttctggct gttcaccgcg atctttgcct cgaccattgc gccttttgat ccgcttggcc
     4741 agtttccgat catgaaggat acgctgcccg gcgcgatcga gccgcaatcg gggctggtct
     4801 atctgttcgg cggcgacaag ctggcccgcg acgtgttctc gcgcatggtc tatggcagcc
     4861 agatcgtcct gatcatcgcg cccgccgcca ccggcttcgc gctgatggtc ggcattacgc
     4921 ttgggctgcc ggccggctat tatggcggca agatcgacac ggtgctgtcg ttcctggcca
     4981 atctcgtgct ggcctttccg gtcatcttgc tgttctacct tttggtcacg ccgggcatca
     5041 tggacacgcc cattccctat gccatggctg gcctgttctt ccttttcccg atcgtctttt
     5101 tctgcgtgtt gttctggacg cggttcaaga accggccaga ccgcatctat atactgatgg
     5161 ggctgacgct gatcctcggc ggctggatct atatggggct ggtcttcaat gccgatccgc
     5221 tcggccttat cagcatggcg ccgaaccagc tcaacatttt cgtggccgtg gtgtttgctt
     5281 ccagtccggg cgttttccgc atcgtgcgcg gcttggtgat ggacatcaag acgcgcgact
     5341 acgtggcggc cgcgcagacg cgcggcgaaa ccccctggta tatcatgctg tgggaaatcc
     5401 tgccgaatgc tcgcgggccg ctgatcgtcg atgcctgcct gcgcatcggc tacaccacca
     5461 tcctgctcgg cacgctcggc tatttcggcc tcggccttgc gcctgaaagc cccgattggg
     5521 gaacggcgat caaggatgcc agccggctgt tgcgccagtt cattcatccg gcgttgccgc
     5581 cgacggtggc gctgatgtcc ttcgtgctgg gattgaacct gctcgccgat gcgttgcgcg
     5641 aacaatcgat gaaagactga tccaccggga gcgtcagcca tgaacgaagc tgttaaagcc
     5701 gcgaacaccg caaacgccga accgatcatc gagatcgaga acctgtctat ctcgtttttc
     5761 acccgcaagg gcgagatacc ggcggtgatg gatttttcct gctcggtgat ggcaggcgag
     5821 gcgatgggca tcgtcggcga aagcggctgc ggcaagtcga cggtttcgct cggcatcatg
     5881 cgcgaccttt ccaacatcgg aaagatcgtc ggcggcaaga tcaagttcca gggcaaggac
     5941 atgggcgaac tgaccgagga ggagcttcgc gccatccgcg gcaacaagat cgccatgatc
     6001 taccaggagc cgatggctag cctcaacccg gcgatgaagg tcggccagca gctgatggaa
     6061 gtgccgctga tccacgacaa ggtgtcgaag gaagaggcct ataagcggtc gctcgaaatg
     6121 gtgcgagcgg tcaagctgcc ggacccggag cgcatgatgc gttccttccc gcaccagctt
     6181 tcaggcggcc agcagcagcg catcgtcatc gccatggcgc tgctttccaa gccggcgctt
     6241 ttgttgctcg acgagccgac tacggcgctc gacgtgacgg tggaggccgg catcgtcgat
     6301 ctggtgcgcg ggcttggcga aaaattcggc acgtcgatga ttttcgtgtc gcataatctc
     6361 ggccttatcc tggaaacctg cgaccgcatc acggtcatgt attcaggcga ggcggtcgag
     6421 accggcaaga tcaaggatgt gttcgaccgc atgcgccacc cctatacgca ggggttgttc
     6481 cgttcgatcc cgctgcccgg cgccgacaag aattcgcgtc cgctgatcgc tattcccggc
     6541 cagttgccgc tgccgcatga acggccgaag ggctgcaatt tcgggccgcg ctgccaccat
     6601 ttcgtggagg gcgtgtgcga cgcggccgaa atcccgatga tccccgtgga ggggcatgac
     6661 ggacattatt cgcgctgcgt gcgtttcaac gagatcgact gggatgcgct gccgcccggc
     6721 gcgcagaagg ttacggagaa ggtcgagcct ggcgcgccgg tgctggaaat cgcggatctg
     6781 cgcaaatatt acaaggtcgg cggcaacgaa gtgttcggcg gcagcgacgg ccgcgtcgtc
     6841 aaggcaaacg agacgatttc gtttaccgcg cgcgaatccg agaccgtcgc catcgtcggc
     6901 gaatccggct gcggaaaatc gacgctggcc aaggtgctgc tcggccttga aaccgccagt
     6961 tccggcaccg tcacgctgga cggcaaggaa atccagtcga ccggcgtcga aagccggaat
     7021 gtcgatacgg tgtcctcgat ccagatggtg ttccagaacc cgttcgatac gctgaacccc
     7081 agccattcgg tcggctcgca gatcatccgc acgctggaaa agttccgtgt cgggaaaaat
     7141 ccgggcgagc gccgccagcg catgctggaa ctgctggacc tggtgaagct gccgcgcgcc
     7201 ttcgagacgc gcaagccgcg ccagctttcg ggcgggcaga agcagcgcat cggcgtggcg
     7261 cgcgcctttg ccggcgacgc taaagtggtg gtggcggacg agccggtgtc ggcgctggac
     7321 gtttcggtgc aggcggcggt gaccgaactg ctgatggaca ttcagcgcaa gaacaagacg
     7381 accatgctgt tcatcagcca cgacctgtcg gtggtgcgct atatcgccga ccgcgtggtg
     7441 gtgatgtatc tgggctacat cgtggagcag ggtacgaccg accagatatt cgcgccgccc
     7501 tatcatccct ataccgaagc tctgctgtcg gcgatcccga ttgccgacac cagcgtggtc
     7561 aagaagcata tcgtgctgga aggcgacatc ccttcggcga tgaacccgcc gagcggttgc
     7621 ccgttccaga cccgctgcgg ctacaagcat ctggtgcccg gcaacaagtg cgaaacgctg
     7681 gtgccgccgg tcaaggatct gggcgacggc catctgagcc tctgctggct cgacgacgac
     7741 gtactggcga tgatggagcc ggtgataagc ttcgacaagg ccgataaggc gcccgtggtc
     7801 gatgcgaccc ccgaagacgg cgatagcgac gaccggcccg ctgacgaagc ggcatcctac
     7861 gatgcgccga agcgtccgcg tggcaagaag gcttctcccg aggctagcgc gttcggggcg
     7921 gcggcggaag aggccgaatc cacagcgcgc gctcgccgcc atgaggtgcg cgacgagaca
     7981 tcggaaaccg gtggcgcttc gacctccggc aattcgacgc tttcgccgtt taccgagcga
     8041 acggttggcg atggcaggca ggcggtggac gagaatgtcg cgccggcttc gaaaccggcc
     8101 agaaagccga aagcggcccc gaagaagaaa tagccggacg ggccggcgct agtcgcattc
     8161 ccgcagctgg acggcgtagt tcctggccat ttcgtcggta tcgcgggcat agcgctgaag
     8221 tgaagcgttg cttttccagc tgccgcgctg ataggcgacc cagccggaat aataggcgag
     8281 gtagagattg taggtgtcgt tcttggcgac gccgagcgtg tcggcggttt tctcgtgata
     8341 ccagccgaca aaatcgaccg cgtcggcgaa gctggtgcgg cgggcggcga agtttccgct
     8401 ctcgcgctta tactgcgccc aggtgccgtc cagcgcctgc gagaagccgg tggcactgga
     8461 agggcgcttc cacggaataa agccgagcag tttcgtgcgc ggcgggcggg cgttggcctt
     8521 gaagccggat tccttgcgca ccgtcgccat caagacggga accggaacac catatttggc
     8581 ttcggcgcgc tttgcggcgg actgccaatt gttgaaccag ccgtcgcgct ggtcgaatac
     8641 ggagcagata ttgttgatgt gacgcggcgc tgtcgcgcag gcggacaagg ccagaagcgc
     8701 gattaccggg acaattttac taaaacgcga cgcatacatg gcgtgagcaa taggggaaaa
     8761 tcataaaggg agtcttgcag cgaatcttaa ccttttggat gccggcgctt gggcattctg
     8821 tgttgatata gcggcgccta aggtcgcttt tatcttcgtc tctccactat cgtggcgctg
     8881 gggctaaaat cacgtccagc catgacgcat tcctgacaga cggcatcggc ggtcgaagct
     8941 tttatgcggc catgagcgaa ccaagaagaa aacgcggcgc cgagcgaacc agcaaccggg
     9001 gtcccgccgc cattccgcaa ctgccgctgc ggcgggtggt caatccctat ccgccgatgg
     9061 ccgtgctgtc ggcggatcag atcgaggcga tccacgaagc ctcgatgcat atactggaaa
     9121 atttcggtat cgaggtgatg tcgccgcgcg cgctttcgct gttcgaaaag gccggcgccg
     9181 aggtcgatca tgcatcgatg aacgtgcgca tcgatcgcgg gctggtggcg caggcgctga
     9241 agacgacgcc ttccgcctat gcgctgaccc cccgaaatcc ggcacatgcc gtgcatcttg
     9301 gcggcaattc catcaatttc acgctggttg ccgggccgcc caacgtccac gacatggagc
     9361 gcggacggcg ctccggaaac atgcgcgatt atggcgatct catccggctg gcgcagcatt
     9421 tcaactgcat ccacatgctg gggaatcagg tctgcgcgcc gatcgaactg ccggcgaatt
     9481 cacgtcatct ggacacttat ttcgccaatt tgacgctgac ggacaagtct ttccacgttt
     9541 ccgccatcgg tcagggccgg gcgctggacg gcatcgagat gatggcgatc agccgtgggc
     9601 tttcgctcga ccagatgcgc gacgatccgg gcctcaccac catcatttcc gtcaattcgc
     9661 cgcgccgctt cgacgacatg atgtcggacg ggctgatgac gatggccgag catggccagt
     9721 cggtggcggt cacgccgttc acgctgatgg gggcgatgag cccggttacg ctggccggcg
     9781 cgctggcgca gcagaatgcc gaggcgctgt tcggcgttgt cctgacgcaa ttggtgcggc
     9841 ccggcgcgcc ggtgatgtat ggcgcgttca cctccaatgt cgacatgaag tcgggcgctc
     9901 cggccttcgg cacgccggag aataccaagg ccaatatcgc gtcgggccag ttggcgcggc
     9961 gctataacct gccataccgc accacgccgg gcagcgcttc caacgccgcc gacgcacaag
    10021 gggcctatga aacgtcgatg gcgctgtggg gcgctatcct tggccacggc aacctcgtct
    10081 accatgctgc cggctggcag gaaggcggac tgactgcctc tttcgaaaaa ttcatcatcg
    10141 atgtcgagat gatccagaac atgatggagg tcctgcgccc catcgaggtc aatgaaggcg
    10201 aactggcgct ggaagcgctg ggcgcggtgc cgaccggcgg gcatttcttc ggcgagccgc
    10261 atacgctgga gcgctattcg acggccttct accagccgat gctgtccaac tggcaaaact
    10321 acgaggcctg ggaggaagcg ggcgggctgg atgccacggc gcgcgccacg cggctgtgga
    10381 agaaggcgct ggaggaatat gtcgagccga cgatggacat tgcggtgcgc gaagaactcg
    10441 aagcctatgt cgccaagcgc aaggaggcga tcgggcaggg cgagccgtaa cggctcatcc
    10501 tccggttctt cgcctcccct cccgaagcgg agaggaggcc gctgccatcg gatcagcgca
    10561 ccggcgccac cagcgcgaaa tgcgcgcctt gcgggtcggt gcagttgacc acccactgtc
    10621 cgccgggcac ttccatcggc cccatcagca ccttgccgcc cttgtcgttg acacgctgca
    10681 cggccgcgtc gatggcgggc acgttgaaat agaattgcca gaccggcacc ggcacttgcg
    10741 gcggcttgtt catcatgccg cccgattgcg cgccgtcgac ggcatagatc tggtaggtgc
    10801 ccatttcgcc catgtcgaag ggatggtctt tggtccagcc gaactggccg gaataaaagt
    10861 ccatcgccgc ctgccagtcc gatgtgtaaa gctcgtgcca accgacatgg ccgggcgtgc
    10921 cggcggccac cggcggcatg tccggtccat tgggctgcaa cagcatgaac atggcgccct
    10981 gcgggtcggc aaccaccgcg aagcggccga cgtcgggaat atccgcaggt tcgcggtgga
    11041 ccttgccgcc cgctttcttc acggcctcgg cggaggcatc ggcatcgctc gaatagatat
    11101 agcctagcca ggcgggcggc atgcccattt cctttgccgc gtccggcaag ttcatcacgc
    11161 cgccgacgcc gcgttcgcca gcattcatga cggtgtagcg cggcatgccg gccgcgccgg
    11221 ggaagggctg cgccgtccat cccaccacat cggtgtagaa tgcctcggcc gcatccatgt
    11281 cggatgtcat cagctcgtac cagaaaaacg aaggggtctt gggcatggca tgctcctgac
    11341 gttggcgttg attgctgttt ggcaatgcaa ggacgtgcgg tgcgccggta aaccgacaag
    11401 ccggacgaga ccatactccg gaccagggcc gttgtccaaa atatgataga aattgtcata
    11461 tttttaggaa gccttttcaa tggcttatcc agaaaattga ccaattgata aaaaaataga
    11521 acgtgctagc ctgcctgaaa atcataataa ccaagagcgg gaaccccaat ggcactcagt
    11581 ctcttcaagg aaggcataga gggcggccgg ctttcaccgg aagcctatgc ggagaatttc
    11641 tccgatctgc atccgccgct ggatcatcac gaggcgctgg tcgagtccga ccgctgctat
    11701 ttctgctatg acgcgccgtg catgaacgcc tgccctacgt cgatcgacat tccgctgttc
    11761 atccgccaaa tctc
//
LOCUS       DASYCS010000324         6163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3567179,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000324 DASYCS010000000
VERSION     DASYCS010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 6163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6163
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3567179"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..177)
                     /locus_tag="VGM46_10140"
     CDS             complement(<1..177)
                     /locus_tag="VGM46_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BA14K family protein"
                     /protein_id="HEY4192992.1"
                     /translation="MNRIFKTAILSTAIAAMTLAALPAANARDWRRHGGYGGYGGYHH
                     SHRGDAVAAGVVGLA"
     gene            complement(359..571)
                     /locus_tag="VGM46_10145"
     CDS             complement(359..571)
                     /locus_tag="VGM46_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002964621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEY4192993.1"
                     /translation="MPQSGTVKFFNHAKGFGFITPDDGEKDVFVHISAVQASGLPGLE
                     DGQKVTFDTEPDKRGKGPKAVNLTVG"
     gene            complement(845..1057)
                     /locus_tag="VGM46_10150"
     CDS             complement(845..1057)
                     /locus_tag="VGM46_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEY4192994.1"
                     /translation="MAQTGTVKFFNATKGFGFITPDDGAKDVFVHISAIEASGLRTLV
                     DGQKVTFDVEPDRMGKGPKAVNLRAE"
     gene            complement(1461..2084)
                     /locus_tag="VGM46_10155"
     CDS             complement(1461..2084)
                     /locus_tag="VGM46_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012763558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEY4192995.1"
                     /translation="MSRLTAFALNCSLKASGDEEKSSTDRMLSDLLAALKPHGVKGEI
                     IRALDHDIKPGVLSDMGKGDEWPKLRARILAADIFILGLPIWLGQPSSVAKRVMERMD
                     AFLAETDDKGRMPAAGKVALVAIVGNEDGAHACHAACFQALNDVGFTIPANAGVYWVG
                     EAMGDVNYVDLPGTPEAVASMIEMAASNAAHLAGALKATAYPGVKSE"
     gene            complement(2196..3209)
                     /gene="gnd"
                     /locus_tag="VGM46_10160"
     CDS             complement(2196..3209)
                     /gene="gnd"
                     /locus_tag="VGM46_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEY4192996.1"
                     /translation="MQIGMIGLGRMGANMVRRLMRDGHECVVYDINAAAVAGLAGEGA
                     VGAASMEEFVQKLQKPRSVWLMLPAAITGQNVDRLAALMEKGDIIIDGGNSNYRDAVD
                     QAAKLQPKGLNYVDVGTSGGVWGLDRGYCLMIGGPEMAVKHLDPIFATLAPQTDLAAA
                     DKTTGGTAQHGYLHCGPSGAGHFVKMVHNGIEYGIMAAYAEGLNILKSANAGNETRTA
                     DAETTPLDEPQYYKFDIDLPAVTEVWRHGSVIGSWLLDLTAGVLKDDPALENFGGRVS
                     DSGEGRWTLKAAIDTGVPAPVLSSALFDRFSSRGQAGFADKILSAMRFAFGGHVEKSK
                     DGK"
     gene            3463..5172
                     /locus_tag="VGM46_10165"
     CDS             3463..5172
                     /locus_tag="VGM46_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEY4192997.1"
                     /translation="MRRREGAKAGAGAGGQPPRGNLDQLVQQALALHQAGRVSEAEAL
                     YVQVLSAKRDHAAALHFFGLLLHQTGRSADGVALIERSLRAAPQNPDFLNNHGSVLRD
                     LGRTAAAADSYRKAVAVKPDHGAARDNLGSALAQLGRFSQAEEVFRGTMGRNPFHMRA
                     RVGLAETMQEAGRLDEALVVFRDALAIRPNDPEVLHGLGVALMEKGDLDGAADMFRRA
                     LAAAPKQAKSWLMLSQVRRQKEADAELAAMQALHKQAGADSLDRMQLSFGLGKLNDDL
                     KAYGAAFDYFAEGNAIRRKGIAYDGEKTRAEFAAMKATFDAAFFEKHKPGAINDDAPI
                     FVVGMPRSGTTLVEQIIASHPQVFGAGELTILKTAVGKGFPLDMKGGFPAGIRELPDK
                     VFADAGHDYLDMLHARYPGFRHVTDKMPGNFLLIGFLHLMLPKAKIVHCARNPVATCL
                     SIYKTHFRGDGHLYSYDLAELADFHNLYTDMMEHWRAVLPGVVHDVRYEDFVADQEGQ
                     TRALIEHLGLPWNDAVLSFHKNDRQVRTASAAQVRQPMYQGSIDLWKNYGDKLQPLIE
                     RLK"
     gene            complement(5245..5598)
                     /locus_tag="VGM46_10170"
     CDS             complement(5245..5598)
                     /locus_tag="VGM46_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006338459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 family protein"
                     /protein_id="HEY4192998.1"
                     /translation="MSYVDGFIVAVPKAKLDAYKDLAHLAGKVWKEHGALAYAECIGD
                     DVPYGELTSFPRAVQATDDEVVIFSWIVYESRESRDAVMAKVMADPRMQPGMENMPFD
                     GKRMIFGGFKPFMGV"
     gene            complement(5624..6097)
                     /locus_tag="VGM46_10175"
     CDS             complement(5624..6097)
                     /locus_tag="VGM46_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004124873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/extradiol
                     dioxygenase family protein"
                     /protein_id="HEY4192999.1"
                     /translation="MSGDDQQPPFPPMPKTLGGLTPYLGIEGATRAAEFYKKAFGAEE
                     AFAVPPDEQGRTMHIHLHINGSSLMLGDFYPEHGHPAKAVQACTMQLHLTEQTIDDWW
                     RRAVDAGCTISTPLQIMFWGDRWGSLRDPFGIDWAMNAPVNSEVNSEVNGHVKTA"
BASE COUNT         1147 a         1858 c         1938 g         1220 t
ORIGIN      
        1 ggccaggccg acgacgccgg cggcaaccgc gtcgccgcgg tggctgtgat gatagccacc
       61 ataaccgccg tagccgccat ggcggcgcca gtcacgggca ttggcagccg gcagcgcggc
      121 cagtgtcatc gcggctatag ccgtcgacag aatggctgtc ttgaaaatcc tgttcatgtt
      181 catctccttc gtgccggcct gccgcatggg aggcgtgaga ccgttgtggt ggagataaat
      241 acaaatccgt ggctgaacga aggctgaacg gaattgtgcg ggaatacgct cgatcccata
      301 tcgaaatcgc cgatggccgg aacttccagc cgccgcaacg gaatgccttg ccggcttttt
      361 aaccgacggt caggttgacg gccttggggc ctttgccgcg cttgtcgggt tcggtgtcga
      421 aagtcacctt ctggccgtcc tcaagtccgg gaagaccgga cgcctgcacg gcggagatgt
      481 gaacgaagac gtccttttct ccgtcatcgg gcgtaataaa gccaaagccc ttggcatgat
      541 tgaagaattt gacggtgccg gactgcggca tggtaggctc ctttaatccc gtcaggtcca
      601 aatttccgtt gggcgcgagc cctgggggga aatttgtcct ttattttaga gccatcggca
      661 agcaaaactt ttggcggggg tgggtgccat tcgagcctgc cgccaattcc tctgggtccg
      721 aatttacctt aaatgacgag cagaagaaga tgatgcgaac agggaaatca acgcaaagaa
      781 ggcgcgccgg aaacccgccg cgccttcttg aaactctatc cgcttgaagt gcggaggacg
      841 cggattattc cgcgcgcaga ttgaccgcct tcggtccctt gcccatgcgg tccggctcga
      901 cgtcgaaggt aaccttctgg ccgtcgacga gcgtgcgcag gcccgaggcc tcgatcgccg
      961 aaatgtggac aaacacgtcc ttggcgccgt cgtcaggcgt aatgaagccg aagcctttgg
     1021 tggcgttgaa gaatttaacg gttccggtct gggccatgtg gggaaactcc ttcacccgtt
     1081 cagtctttgg tcctgcccgc cgcctggcgt cgggagcaag ttccggtggt tgcttcggcg
     1141 gtcatgcatt tgcgcatgac aaaaaccccc gccgaaaacg gggccgtcag agattcacaa
     1201 atatcgggga aagggttgtg caaaacgttc cagtctccgg gtccgtaaat gcccgaacac
     1261 tggatttatg gcatggaaat ccagaggtgg caagacgggc acggtgaacc ggcgcagctc
     1321 ggcatcctcc gcatagccgc cgtttggcgg aaggtcatca gccagatggc ttgaaatatg
     1381 ggtgcagcat cggccagacg gccatgttga cacgcgaacg aatggccgat tttggcagac
     1441 cgcaccgggt ttgacacggt ctattcggat ttcacgccgg gataggccgt tgccttcagc
     1501 gcgccggcca gatgcgccgc attcgacgcg gccatttcga tcatggaagc taccgcctcg
     1561 ggtgttccgg gcaagtcgac ataattgaca tcgcccatcg cctcgccgac ccagtagacg
     1621 ccggcattgg cggggatggt gaagccgaca tcgttgagcg cctggaaaca ggcggcgtgg
     1681 caggcgtggg cgccatcctc attgccgaca atggccacca acgccacctt gccggcggct
     1741 ggcatcctgc ccttatcgtc ggtttctgca agaaacgcgt ccatgcgctc catcacgcgc
     1801 ttggccacac ttgaaggctg acccagccag atcggcaggc caaggatgaa aatatctgcg
     1861 gctaaaatcc tggcgcgcag tttcggccat tcgtcgccct tgcccatgtc ggacagcacg
     1921 cccggcttta tgtcatggtc gagggcgcga atgatttcgc ccttcacgcc atgcggcttc
     1981 agcgcggcaa gcagatcgga cagcatccgg tcggtcgatg atttctcttc gtcgccggat
     2041 gctttgagcg agcagttgag ggcaaaggcg gtcaatctgg acatgaaatc tcctgtattc
     2101 gcatcaggag aacgtgaggg cggcgcagag gttgcgcgcg ccctcatcaa gtcttgtgcg
     2161 ccgcgccaag caagggcgct atccgaagct ggcgcttact tgccgtcttt cgatttttcg
     2221 acatggccgc cgaaggcgaa gcgcatcgcc gacaagatct tgtcggcaaa tccggcttgg
     2281 ccgcgcgatg aaaagcggtc gaacagtgcg gatgacagca ccggagccgg cacgccggtg
     2341 tcgattgccg ccttcagcgt ccagcgccct tcgccggaat ccgagacgcg gccgccgaaa
     2401 ttctccagcg ccgggtcgtc cttgagcacg ccggcggtca ggtcgagcag ccatgaaccg
     2461 atgacgctgc cgtgccgcca cacttcagtc acggcgggaa ggtcgatgtc gaacttgtag
     2521 tattgcggtt cgtccaacgg ggtggtttcg gcgtcggcgg tgcgcgtttc attgccggca
     2581 ttggcggatt tcagtatgtt cagcccctcg gcataggcgg ccatgatgcc gtattcgatg
     2641 ccgttatgga ccatcttgac gaaatggccg gcgccgctcg gtccacaatg gaggtagccg
     2701 tgctgggcgg tgccgcctgt cgttttgtcc gccgccgcca gatccgtttg gggagccagc
     2761 gtcgcgaaga tcgggtcgag atgcttcacc gccatctccg ggccgccgat catcaggcaa
     2821 tagccgcgat ccagccccca gacgccgccg ctggtgccga catcgacgta attcaggccc
     2881 ttcggctgga gttttgccgc ctgatcgacg gcatcgcggt aattggagtt gccgccatcg
     2941 atgatgatgt cgcccttttc catcagcgcc gccaggcggt cgacgttctg gccggtaatg
     3001 gcggctggca gcatcagcca gacgctgcgc ggcttttgca gtttctggac gaactcttcc
     3061 atcgaggcgg cgccaaccgc gccttcgccg gcaaggccgg ccacggccgc ggcattgatg
     3121 tcgtagacga cgcattcatg gccgtcgcgc atcaggcggc gcaccatgtt cgcgcccatc
     3181 ctgccaagcc caatcattcc gatttgcatt cttcagtcct gttcgttgct ggtctgcgct
     3241 gccggatcgc ggcaacaagc catcgatgca tcaccttttc cgataaaccg gcccgcttgg
     3301 gaagctcgat tacgccgtca tgcgtgacag tgtcgtgatc acagcccttg tgcgggtctc
     3361 gcgccattat aggatagagc cagttcaacc ttgcatcgca ggcagtgatg ccctagttcg
     3421 cggcaaacgg acaaaaatgg caggttcatc atcaaccggg cattgagacg acgggaaggg
     3481 gccaaggctg gcgcgggtgc gggcggccag ccgccgcgcg gcaatctcga ccagctcgtg
     3541 cagcaggcgc tggcgctgca tcaggcgggc cgggtcagcg aagccgaggc gctgtatgtt
     3601 caggtgctgt cggcgaaacg ggaccatgcg gcggcgctgc attttttcgg gcttttgctg
     3661 caccagaccg gccgcagcgc cgatggcgtc gcgctgatcg agcgatcctt gcgcgccgcg
     3721 ccgcaaaacc cggattttct caacaatcat ggctcggtgc tgcgcgatct cggacggacg
     3781 gcggcggctg ccgattccta ccgcaaggcg gtcgccgtca agccggacca tggcgcggcg
     3841 cgcgataatc ttggttccgc gctggcgcaa ctcggacgct tttcgcaagc tgaggaagtt
     3901 tttcgcggca cgatgggacg caatcccttc cacatgcgcg cacgcgtcgg gctggcggag
     3961 acgatgcagg aagccgggcg gctggacgag gcgcttgtgg tcttccgcga cgccctggcg
     4021 atccgcccca acgatccgga agtcctgcat gggctgggcg ttgcgctgat ggaaaaaggc
     4081 gaccttgacg gtgcggccga catgttccgg cgcgcgctgg ccgctgcgcc gaagcaggcg
     4141 aagtcgtggc tgatgctaag ccaggtgcgg cgccagaagg aggccgacgc cgaacttgcg
     4201 gccatgcagg cgctgcacaa gcaggcgggc gcggacagtc ttgaccggat gcaactatcc
     4261 ttcggcctcg gcaaactcaa cgacgacctc aaggcctatg gcgctgcctt cgactatttt
     4321 gccgaaggca atgccattcg ccgcaagggc atcgcctatg acggcgaaaa gacgcgcgcc
     4381 gaatttgccg cgatgaaggc gacattcgac gccgcgttct tcgagaagca caagccaggc
     4441 gctatcaacg atgacgcgcc gatcttcgtc gtcggcatgc cgcgttcagg cacgacgctt
     4501 gtcgagcaga ttatcgccag ccatccgcag gtgttcggcg ccggcgagct gaccatcctg
     4561 aagacagccg taggcaaagg cttcccgctc gacatgaagg gcggctttcc ggccggtatt
     4621 cgggaattgc ccgacaaggt attcgcagac gccgggcatg attatctcga catgctgcat
     4681 gcccgttatc cgggcttcag gcacgtcacc gacaagatgc cgggcaactt cctgctgatc
     4741 ggcttcctgc atttgatgtt gccgaaggca aagatcgtcc actgcgcgcg caacccggtg
     4801 gcgacatgtc tgtcgatcta caagacgcat ttccgcggcg acggccatct ctacagctac
     4861 gaccttgccg agcttgccga tttccacaat ctctacaccg acatgatgga acattggcga
     4921 gcggtgctgc cgggcgtggt gcatgatgtg cgctacgagg atttcgtcgc cgaccaggaa
     4981 ggccagaccc gcgcgctgat cgagcatctt ggcctgccgt ggaacgatgc ggtgctgtcg
     5041 ttccacaaga acgaccggca ggtgcgcacg gcttccgccg cgcaggtgcg ccagccgatg
     5101 tatcaaggtt cgatcgacct ctggaaaaat tatggcgaca agcttcagcc gctgatcgag
     5161 cgactgaagt aagtttcttc tgggcaggcg gtgggccgca ggccatgcgg cccctctgtc
     5221 cgacctccag caaggaaggc ggggctacac tcccatgaag ggcttgaagc cgccgaagat
     5281 catgcgcttg ccgtcgaagg gcatgttttc catgccgggt tgcatgcgcg gatcggccat
     5341 gaccttggcc atgaccgcat cgcggctttc gcgggattcg tagacgatcc acgaaaagat
     5401 caccacctcg tcgtccgtgg cctgcacggc gcgcgggaat gaggtgagtt cgccataagg
     5461 cacgtcgtcg ccgatgcatt cggcataggc gagcgcgcca tgctccttcc agaccttgcc
     5521 ggccagatgg gcaagatcct tgtaggcgtc gagttttgcc ttcggcacgg caacgatgaa
     5581 accgtcgaca taagacattt ttctatctcc tttggttggt ggttcaggcg gttttcacat
     5641 ggccgttcac ttcgctgttc acttcgctgt tcactggggc gttcatggcc caatcgatgc
     5701 cgaaaggatc gcgcagcgag ccccagcggt cgccccagaa catgatttgt agcggcgtgc
     5761 tgatggtgca gccggcatcg accgcgcgcc gccaccaatc gtcgatggtc tgttcggtca
     5821 aatgaagctg catggtgcag gcctgcacgg ccttcgccgg gtggccgtgc tcgggataga
     5881 agtcgccgag catcaggctg gagccgttga tgtggaggtg gatatgcatg gtgcggccct
     5941 gctcgtcggg cggcacggca aatgcctctt cggcgccgaa agccttcttg tagaattccg
     6001 ccgccctggt cgcgccttcg atgcccagat agggcgtcag gccgccgagc gttttcggca
     6061 tcggcggaaa gggcggctgc tggtcgtcac cggacatgct gttctcctgt ctttggcggt
     6121 ttccacctcc acaggacgcg tggtgaacgc ccgatccgac aag
//
LOCUS       DASYCS010000325         4206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1828237,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000325 DASYCS010000000
VERSION     DASYCS010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4206
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1828237"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="VGM46_10180"
     CDS             complement(<1..752)
                     /locus_tag="VGM46_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193000.1"
                     /translation="MKAGLTTSVILHAAVLAFGLFSLSAPAALEVTDTESLPVDIVPL
                     SELTQIQQGDKKADMKDKPAPMPTKRPDIVADAQKVGENSVDTDKPVTPEATPRPVEE
                     AAAPKPAPEPKEQPKTEDVPKPKEEAKPVPATEVTPLPQPKEEVKPEPVKQPEPKPEP
                     VKQPEPKQQPPEEKTAEPQPKEEVKPDEVAKAIAAEPPAEAVKLPDAAPAPDARPKPQ
                     PAQAETAKAPDRKDSEKPVKEASSRPKSDEKE"
     gene            complement(755..1204)
                     /gene="tolR"
                     /locus_tag="VGM46_10185"
     CDS             complement(755..1204)
                     /gene="tolR"
                     /locus_tag="VGM46_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HEY4193001.1"
                     /translation="MSMSGGSGRGGRGHRRRGRHHALMSEINVTPMVDVMLVLLIIFM
                     VAAPLLTVGVPIDLPDTQAKAMNADTQPITVSINDTGKIYLQETEIPIDEVVAKLQAI
                     SKTGYDERIFVRGDKTADYGTVMKVMARISAAGYKNIGLVTLQEQDQ"
     gene            complement(1219..1929)
                     /gene="tolQ"
                     /locus_tag="VGM46_10190"
     CDS             complement(1219..1929)
                     /gene="tolQ"
                     /locus_tag="VGM46_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HEY4193002.1"
                     /translation="MENIALAEPGAHLSIWALFMQASWVVKLVMLGLVAASIWSWAII
                     VDKLVSYARMRAALNRFEQIFWSGQSLEELYRTLADRKTTGMGAIFVAAMREWKKSFE
                     KGAKSPLGLQNRIDKAMDLALTREMEKLEGRLGFLATTGSAAPFIGLFGTVIGIMTSF
                     QAIAASKNTSLSVVAPGIAEALLATAIGLLAAIPAVIAYNKLSSDANKIAVKMEGFSD
                     EFSAILSRQIDEKVAQKV"
     gene            complement(2193..2660)
                     /gene="ybgC"
                     /locus_tag="VGM46_10195"
     CDS             complement(2193..2660)
                     /gene="ybgC"
                     /locus_tag="VGM46_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006327715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HEY4193003.1"
                     /translation="MDDHGESDTALAAGLSGALTASGHRLLARVYYADTDFSGVVYHA
                     RYLEFLERGRSDFLRLAGVHHTELAGGKHGERIVWVVRRMEIDFRAPARIDDILTVDT
                     RASEISGARIVMLQQLRRGAELLVEATVHAAIIGENGKPRRFPKEWIAAFLRQ"
     gene            2713..3975
                     /locus_tag="VGM46_10200"
     CDS             2713..3975
                     /locus_tag="VGM46_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEY4193004.1"
                     /translation="MAPLMTTIKTLMMALLLSLPLGLPLGLPSQAASFTMKRGLNLDP
                     WTTWAGEDKWGDPAVMLPFPEWRKFLGDGELKALKDAGLDFLRMPVDPSPFLSDKTLP
                     LRDGLYASVLESARTINRAGLKVVVDLHLTPSGGNGTIGMAEVMADPAMFERYLELVR
                     TMAGTLAGENPSLVAFEPMNEPIVDCEGDGTDLWPQRLKRLFAAARASATGLTLVLSG
                     SCYSSADGLAKIDPKEIPDGNVIWTFHSYQPFLLTHQGATWAGDFIRYVTGLPYPLNA
                     VSSEQLEATLDTIRQKIKDEAPWSRRAGLLAYLDEQVAGIDSQQKLLAEMAAPFKQAE
                     AWAKANGIRPENVTLGEFGMIRQEYGNPYVMPGKYRAAYVHDMIGLAEKNGFSWSIWE
                     YGGSFGIVDAFDGKKAEPDVMEMVRALK"
     gene            complement(4078..>4206)
                     /locus_tag="VGM46_10205"
     CDS             complement(4078..>4206)
                     /locus_tag="VGM46_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006200120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HEY4193005.1"
                     /translation="LIQQGFIQRTPRGRVLTANAWRHLGLDAPRDLAQQQINLFQE"
BASE COUNT          714 a         1268 c         1366 g          858 t
ORIGIN      
        1 aattccttct cgtcggattt gggccgcgag gacgcttcct tgaccggctt ttccgaatcc
       61 ttgcggtcgg gcgccttggc ggtttcggct tgcgccggct gcggcttcgg gcgtgcatcg
      121 ggcgccggcg ccgcatcggg aagcttgacg gcttcggcag gcggctcggc ggcgatggcc
      181 ttcgccacct cgtccggctt gacctcttcc ttcggctgcg gctcggcggt cttttcctcg
      241 ggcggctgct gcttcggttc cggctgcttc acaggctccg gcttgggctc gggctgcttg
      301 accggctcgg gcttgacctc ttccttgggt tggggaagcg gcgtcacctc ggtcgcggga
      361 acgggctttg cctcttcctt cggcttggga acgtcctcgg tctttggctg ttccttcggt
      421 tccggcgcgg gtttcggggc ggctgcctcc tccaccggcc tgggtgtcgc ctccggcgtt
      481 accggcttgt cggtgtcgac gctgttttcg ccgacctttt gcgcatcggc gacgatatcg
      541 ggacgcttgg tcggcatcgg cgccggcttg tccttcatgt cggccttttt gtcgccctgc
      601 tggatctggg taagttcgga caagggaacg atatcgaccg gcagcgactc ggtatcggtc
      661 acttccagcg cagcaggagc cgacagcgaa aacagcccga aggccagcac cgccgcatgc
      721 aagatcaccg atgtggtgag gcccgccttc aagattactg atcctgttcc tgcaaggtga
      781 cgaggccgat attcttgtat ccagccgctg aaattcgggc catgaccttc atcaccgtgc
      841 catagtcggc ggtcttgtcg ccgcgcacga agatgcgctc gtcataaccc gtcttggaga
      901 ttgcctgtag cttggccacc acctcgtcga tggggatttc ggtttcctgg agatagatct
      961 tgccggtgtc gttgatcgag acggtgatcg gctgggtgtc ggcgttcatg gccttggcct
     1021 gcgtgtcggg caggtcgatc ggcacgccga ccgtcagcag cggagcggcg accatgaaga
     1081 tgatcagaag caccagcatc acgtcgacca tcggcgtgac gttgatttcc gacatcagcg
     1141 cgtgatggcg gccgcgccgc ctgtggccac gcccgccgcg gccggagccg ccactcatcg
     1201 acattcccat ggctggtcct aaactttctg cgctactttt tcatcgattt ggcgcgagag
     1261 tatggcggag aactcgtcgg agaacccttc catcttcacg gcgatcttgt tggcatccga
     1321 tgacagcttg ttgtaggcga tgaccgccgg aatggcggcg agcaggccga tggcggtggc
     1381 cagcagcgcc tccgcgatgc cgggagccac taccgaaagg ctggtgttct tcgaggcggc
     1441 aatggcctgg aaggaggtca tgatgccgat gaccgtgccg aacaggccga tgaagggagc
     1501 tgccgaaccg gtggtggcga gaaagccgag ccggccttcc agcttctcca tctcgcgggt
     1561 cagcgccagg tccatcgcct tgtcgatgcg gttttgcagg ccaagcggcg acttggcgcc
     1621 cttttcgaag cttttcttcc attcgcgcat cgcggcgacg aaaattgcgc ccatgccggt
     1681 ggtcttgcgg tccgccagcg tgcggtaaag ctcctccagc gactggccgg accagaatat
     1741 ctgctcgaag cggttgagcg cggcacgcat gcgcgcatag gaaacgagct tgtcgacgat
     1801 gatcgcccag ctccatattg aagcggcgac cagccccagc atgaccagct tgaccaccca
     1861 gctggcctgc atgaacagcg cccaaatcga caaatgcgcg cccggttcgg cgagtgcgat
     1921 attttccatg gtgaagtcct taagattttc cgggcgtggc cgtcggctgc cccagcgcat
     1981 gatcccttgt ggcccgttcg cgcgaagctt ccggtttgcc caaaaggcgc ggtaagcggc
     2041 cttttcagca caaatattgg ttaaacgaag gcagctattg cctcagcttt cgtcaaaccc
     2101 ttcatgaacc gttatggtta aggatcggtt agggttgcgc cgccgcccgc cggtagccgt
     2161 tctcgccccg atgcgctgcg aaaggcagcc gtctattggc gcaggaaggc ggcgatccat
     2221 tccttgggaa agcgccgcgg cttgccgttt tcgccgatga tggcggcgtg cacggtggcc
     2281 tcgaccagca gttccgcacc gcgccgcaat tgctggagca tgacgatgcg ggcgcctgaa
     2341 atctcggaag cgcgtgtatc cacggtcagg atatcgtcga tgcgggcggg cgcgcggaag
     2401 tctatttcca tgcgccggac cacccagacg atccgttcgc catgcttgcc gccggcaagt
     2461 tcggtgtggt gaacgccggc cagccgcaag aagtcggaac ggccgcgctc caggaattcc
     2521 aggtagcgcg catgatagac gacgccggaa aaatcggtat cggcatagta tacgcgcgcc
     2581 agcagccggt gtccggaagc cgtcagcgcg ccggaaaggc cggcggcaag cgccgtatcc
     2641 gattcaccat gatcgtccat aatgctttcc tttttgccgt cgagcgggca ggcccatctc
     2701 caagagaatg cgatggcacc gttgatgacg acgatcaaga ccctgatgat ggcgctgctg
     2761 cttagcctgc ccctgggctt gcccttgggg ttgccttcgc aggcggcgag ttttacgatg
     2821 aagcgcggcc tcaacctcga tccatggacg acatgggccg gcgaggataa atggggcgac
     2881 cccgccgtca tgcttccgtt cccggaatgg cgcaaattcc ttggcgatgg cgagctgaag
     2941 gcgttgaagg atgcggggct ggatttcctg cgcatgccgg tcgatccgtc gcctttcctg
     3001 tcggacaaga cgttgccgtt gcgcgacggg ctatatgcca gcgtgctgga atcggcccgg
     3061 accatcaacc gggccggatt gaaggtggtc gtagacctgc atctgacgcc gtccggcggc
     3121 aatggcacca tcggcatggc cgaggtgatg gccgatccgg ccatgttcga gcgctatcta
     3181 gaattggtgc gaaccatggc gggcacgctt gccggcgaaa atccctcgct tgtcgccttc
     3241 gagccgatga acgagccgat cgtggattgc gagggcgacg gcaccgattt gtggccgcag
     3301 cggctgaagc gactgttcgc cgccgcgcgc gcttcggcga ccgggctgac gctggtgctg
     3361 agcgggtcct gctattcaag cgcggacggg ctggcgaaga tcgacccgaa ggagataccg
     3421 gacggtaatg tcatctggac gtttcattcc taccagccgt tcctgcttac ccatcagggc
     3481 gctacctggg ccggcgattt cattcgatat gtcaccgggc tgccctatcc gctgaacgcc
     3541 gtatccagcg aacagctgga agcgacgctg gacaccatcc ggcagaagat caaggatgaa
     3601 gcgccgtggt cgcgccgcgc cgggcttctc gcctatctgg acgaacaggt cgccggcatc
     3661 gacagccaac agaagctgct tgccgaaatg gccgcgccgt tcaagcaggc cgaggcgtgg
     3721 gccaaggcca acggcatcag gccggaaaac gttacgctgg gcgagttcgg catgatccgg
     3781 caggaatatg gcaaccccta tgtgatgccg gggaaatatc gcgccgccta cgtccacgac
     3841 atgatcgggc ttgccgaaaa aaacggcttc tcatggtcga tctgggaata tggcggctcg
     3901 ttcggcatcg tcgatgcctt tgacggcaag aaggccgagc cggatgtgat ggagatggtg
     3961 cgggcgctga agtgagcaag cctgtgccag atgccgcccc acgtcagggg ctgcgtgttc
     4021 tgtcgctgtg aaaaatttcg ccagggcgtt cggttcgcct gcggcaagat cggcgtctca
     4081 ctcctggaac aggtttatct gctgctgggc cagatctctg ggggcatcga ggccgaggtg
     4141 acgccaggcg ttggctgtca gcacgcggcc gcgcggcgta cgctggatga agccctgctg
     4201 gatcag
//
LOCUS       DASYCS010000326        12734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_5162357,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000326 DASYCS010000000
VERSION     DASYCS010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5162357"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="VGM46_10210"
     CDS             complement(<1..608)
                     /locus_tag="VGM46_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006330664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY4193006.1"
                     /translation="MNRSLSRRSVLSSLASVSAVVMLAACSDSGEEAKAAEVKPGNAM
                     KPADAMKPATNAMKPADAMKPAVAAPESSGSVDMAELLKPGALPEKSLGKDDAKVTVV
                     EYASMTCPHCAHFAETTFPEMKTKYIDTGKVRYILREFPFEPRAEAGFMLARCAGDNY
                     FPMVDVLFKQQQNWVAVDNVKDALLQLSKLAGFTQDSFDKCLT"
     gene            complement(747..1244)
                     /locus_tag="VGM46_10215"
     CDS             complement(747..1244)
                     /locus_tag="VGM46_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008839372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEY4193007.1"
                     /translation="MAGKKPDRNPVAVSDLTARILDPVLRKRAGISIGLVQSWDEIAG
                     PRLSRHSRPEKIQWPRRMHEDDPFEPAVLVIACEGMAALHLQHETSEVIGRVNAFLGF
                     NAVGRIKIVQKPLTAEKVKQKPSFRPLSAPEQSKLAGTVRLIEDDGLRASLERLGATI
                     LGERK"
     gene            1287..2399
                     /gene="mutY"
                     /locus_tag="VGM46_10220"
     CDS             1287..2399
                     /gene="mutY"
                     /locus_tag="VGM46_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEY4193008.1"
                     /translation="MAPQPQTRKAKPSNEGPVAARLLDWYDAHHRELPWRVPPRDLAH
                     GIRPDPYRIWLSEVMLQQTGVETVKAYFSAFVAKWPTVEALAAATSEDVMKAWAGLGY
                     YSRARNLKACADLVAASGGKFPDIEDGLRALPGIGAYTAAAVAAIAFDRPATVVDGNI
                     ERVVSRLFSIATPLPQAKVEIRERVGQLTPEARPGDFAQAMMDLGATICTPRKPRCML
                     CPLREDCDALASGDPEFFPVRASKPDKPARRGAAFVAVRGDGAILLRKRPDKGLLGGM
                     SEVPTTGWTARTDGETTPNAAPFAGDWKPMGSIGHVFTHFSLELEVFRADADIAAPAD
                     AFWSLPGDIAGEALPTVMKKAIEAAIPGATKKPRPQ"
     gene            2410..3021
                     /locus_tag="VGM46_10225"
     CDS             2410..3021
                     /locus_tag="VGM46_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEY4193009.1"
                     /translation="MTEIRHIVFDIGKVLIHYDPNIPFSRLIPDEAERKWFFENVCTH
                     EWNIEQDRGRTWAEAEAHAIAAHPGHADNIRNFRRHWSAMVPHAYEDSVALMDGLIDA
                     GHDVTMLTNFAADTFVEARRRFPFLHRPRGVTVSGEIRQIKPDRAIYDHHTASFSLDP
                     AATLFIDDSQPNVDGAKAAGWQAVLFTGADKLKSDLKTYGIAA"
     gene            complement(3124..4254)
                     /locus_tag="VGM46_10230"
     CDS             complement(3124..4254)
                     /locus_tag="VGM46_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008839369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEY4193010.1"
                     /translation="MSAVRLLADLPHASQQSDWLDTILKGDCVAALERLPEKSIDVIF
                     ADPPYNLQLDGDLHRPDQSKVDAVDDAWDQFESFEAYDAFTRAWLLAARRVLKPNGTI
                     WVIGSYHNIFRVGAKLQDLGFWILNDIVWRKTNPMPNFRGRRFQNAHETMIWASRDQK
                     GKGYTFNYEALKASNDDIQMRSDWLFPICTGGERLKNDNGDKLHPTQKPEALLARIMM
                     ASTKPGDIVLDPFFGSGTTGAVARRLGRHFVGIEREQAYIDAAKERIDAVVPLHGADL
                     TVLTGKRAEPRVAFVSLLDTGLMTPGATLYDSRKRFAAKVRADGTVAIGDSAGSIHRM
                     GAQVQGLDACNGWTFWHYERNGGLTPIDELRRIARLGMERAG"
     gene            4430..5116
                     /locus_tag="VGM46_10235"
     CDS             4430..5116
                     /locus_tag="VGM46_10235"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006330653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY4193011.1"
                     /translation="MPLPELVIFDCDGVLVDSEIIAARVESELLTASGFEISAEELAE
                     TYAGLTFKDILIKVEEISKIPFQISLIDRAEELIDRKLRTDVRAIEGVHEAVAAVPVP
                     FCICSNSRTERIELMLEKVRLLPLFSGRIFSALETPTGKPKPAPDVFLHAAEKLGADP
                     ARCFVVEDSVHGVAGAVAAGMRVIGFTGASHSYPGHADALTEAGAETVIRRWPELKSV
                     IAALSEWSDA"
     gene            complement(5145..5735)
                     /locus_tag="VGM46_10240"
     CDS             complement(5145..5735)
                     /locus_tag="VGM46_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019169803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193012.1"
                     /translation="MLAVAGCQSGDKGVLGIGGGGSQQAKVEDTRVKASDLMAYCPTI
                     TLRDGTAYFNTYANGAKKKVKKADDAEADAAPAADAEDQSANIVYQAAITDVTRDCAR
                     NAGTLTMNVAVAGKVVPGPKATAGSITMPIRIAVLEGSQVIYSQLHQYKLQVTDMSAA
                     TQFVFSDPNVSVPEPSSKNYQVFAGYDEGPPAKKKK"
     gene            5921..7315
                     /gene="thrC"
                     /locus_tag="VGM46_10245"
     CDS             5921..7315
                     /gene="thrC"
                     /locus_tag="VGM46_10245"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010914908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEY4193013.1"
                     /translation="MHYVSTRGEAPVLGFSDTIMAGLARDGGLYVPREWPHFSTAEIR
                     AMRGLSYPDLAIRVLTPFLGGEIAAPVFERLVREAYATFRHEATCPLVQLGTNSFVLE
                     LFHGPTLAFKDVAMQLLARLMDHVLAERGQHATIVGATSGDTGGAAIDAFAGRERTDI
                     FILFPHGKVSPVQQRQMTTSSAANVHALAVEGNFDDCQSLVKGMFNDHAFRDRVALSG
                     VNSINWARIMAQIVYYFSSALSLGSPDRPISFTVPTGNFGDIFAGYAAKKMGLPIERL
                     VIATNDNDILARTLASGEYRTKGVISTTSPSMDIQVSSNFERLLFEASNRDSGKVRRY
                     MDGLKQSGAFTIEADGLAGIRAEFDAGRATMEETAATIRSTLEGSNYLLDPHTAAAVH
                     VAARRTGEVPMVVLGTAHPAKFPAAVEAASGVSPALPSWLSGLMSAEEKFSVLPSDLK
                     MLEDYVGRHTRAAR"
     gene            7330..8622
                     /locus_tag="VGM46_10250"
     CDS             7330..8622
                     /locus_tag="VGM46_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002963641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY4193014.1"
                     /translation="MGVEVSRLSNGLTVATETLPSIESVALGVWIKSGARNERDEEHG
                     MAHLLEHMAFKGTKRRTAFEIASEIENVGGEINAATSVETTSYFARVLSADVPLAVDI
                     LADILQESEFDPHELEREQHVILQEIGAAHDTPDDVVFDHFTETAFRHQTLGRSILGT
                     PETVKSFTSTQLHDYIGRQYGAERMVVVAAGDVKHDDFVREVEKRLGGFRSKAESIMP
                     QYAQYVGGDFRENRDLMDAQIVLGFEGRAYHVRDFYASQVLSMILGGGMSSRLFQEVR
                     EKRGLCYSVYAFHWGFSDTGIFGVHAATGQSSLGELVPVIMNELQRTGENILQEELDR
                     ARAQYRAGLIMSAESPSSRASQIARQLLLFGRPVAKEELMERLDALTTGRLTDLSGRL
                     FSTRPTLTAVGPVGTLAPYEELLDLVSGSQTAARKLAV"
     gene            8683..9222
                     /locus_tag="VGM46_10255"
     CDS             8683..9222
                     /locus_tag="VGM46_10255"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006206326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY4193015.1"
                     /translation="MLRVPSPGDYREWSTLRGESRGFLEPWEPRWTPEELERHAWRHR
                     IHRYREDYALGLSMAFFIFELGSGKLVGGITLGNIRHGVSQSGHVGYWIGERYAGRGL
                     MTEAVNVLAQFAFDTLRLHRIEAACIPDNARSIRVLEKAGFRREGLLRSYLRINGFWQ
                     DHYLYARIADDGPGERTKG"
     gene            9226..12114
                     /locus_tag="VGM46_10260"
     CDS             9226..12114
                     /locus_tag="VGM46_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY4193016.1"
                     /translation="MTNSPRIRSLLAFVLAAIVTFCSVTATFAVEPIKIARDDVALDL
                     SGAVEIYRNQGENFQVSTAPGPDGIVRRIEVEANDKRSTGDWAVFALANTTDQQLDRL
                     IVAPHFRLVNSGIFWPDLGSTRIAAITPSEGFALDRQTSPDADVFRVTLNPGTVVTFI
                     AELASPKLPQVYLWEPESYKDTVNSYTLYRGIVIGIAGLLALFLTILFVVKGTSMFPA
                     TAALAWAVLAYICIDFGFLNKVIEISPGSEQMWRASTEVGLATTFVVFLFTYLNLNRW
                     HGHFSYGALAWVAGLVLIAGVAIIDPAIAAGIARISFAATAIIGLCLIVYLGIRGYDR
                     AIMLVPSWVMVLLWLAGSWMAITGVLDNDIAQPALGGGLILIILLIGFTVMQHAFAGG
                     ALHQGLFSDLERQAMAIIGSGDTVWDWDVLRDRVVTRPDISLQLGLEPHSLSGAARNW
                     LPVLHADDRDTFRTTLDVVLEHRRGKVAQNFRLRGADGHYHWFALRARPVIGSDGQVI
                     RCVGTMIDVTEQKKSEERLLHDAVHDNLTGLPNRELLMNRLETIISIARTEEKVRPTV
                     FVIDIDRFKQVNDGLGISAGDTILLTVARRLHRLLMPKDSLSRFAGDQFALMLLSEQE
                     PGRIAAVADAIKHAINAPITFAKREIVLTASIGLISWTSEQTSAEDMVKDAELAMHQA
                     KRFGGDRIEPFRPAFRTVGSDRLQFESDLRRAVERREFTLAYQPIVRLEDNSIAGFEA
                     LLRWDHPRRGMIPPADFIPVAESCGLIVQLGLFAMQKAAEDLAAWQKQISDAPLSVSV
                     NLSSRHLIRRDLVSDVRSVIARANLKPRCFRLELTESLVMDNPEQAVHVLNKLKQLGV
                     GLSLDDFGTGYSSLSYLTRFPFDTIKIDKSFVDDNTPKRSVLLRSMVNMAHELGLSVV
                     VEGISDQKDALELRQMGCEYVQSFMFGPPVAGDIVLKMLKEQYPLTQA"
     gene            complement(12195..>12734)
                     /locus_tag="VGM46_10265"
     CDS             complement(12195..>12734)
                     /locus_tag="VGM46_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEY4193017.1"
                     /translation="MPGMKDDRFARAVIYVCAHSEEGAMGLIVNQTQQMLFPDLLVQL
                     GILDEQEAIRLPAPARDFVVRNGGPVDRSRGFVLHSGDYKVESSLAVSDDICLTATVD
                     ILRAISSGRGPRQALMALGYAGWGAGQLESEIAGNGWLTCPATPELLFDADIDRKYDK
                     ALASIGIDLAHLSGAAGHA"
BASE COUNT         2303 a         4060 c         3961 g         2410 t
ORIGIN      
        1 gtcaagcatt tgtcgaagct atcctgtgta aaaccggcga gcttggaaag ctgcaacagc
       61 gcatccttga cgttatcaac ggccacccag ttctgctgct gcttgaacag cacgtcgacc
      121 atcgggaaat agttgtcgcc cgcgcagcgc gccagcatga agccggcttc ggcgcgcggc
      181 tcgaacggga attcgcgcag gatgtagcgc accttgccgg tgtcgatata tttcgtcttc
      241 atttccggaa aggtcgtctc ggcgaaatgc gcgcaatgcg ggcaggtcat cgaggcatat
      301 tcgacgaccg tgaccttggc atcgtccttg ccgagggact tttcaggcag ggcacccggc
      361 ttcagcaatt cggccatgtc gacggaaccg gaggattccg gcgcggcgac ggccggcttc
      421 atggcgtcgg ccggcttcat ggcattggtg gcgggcttca tcgcgtcagc cggcttcatg
      481 gcgttgccgg gcttgacctc ggctgccttg gcttcctcgc ctgaatcgct acaggcagcg
      541 agcataacca ccgccgacac ggaggccagc gaagacagga cggacctgcg ggacaaggaa
      601 cggttcattc gaatcacccg gttgataatg gatttcagac tttgcaacgc aaaggcacga
      661 gaaagcgaga gttggcgcga attaaggcat cacgcgaacc aatccaaccg cgaaaagcgg
      721 tggcgggatc acgaatacgc gatgcgctac ttccgttcgc ccagaatagt ggcgcccagc
      781 cgctccagcg aggcgcgcag gccgtcatcc tcgatcagcc gcacggtgcc ggccagtttc
      841 gattgttccg gcgccgaaag cggcctgaac gaaggcttct gcttcacctt ttcggccgtc
      901 agcggctttt gcacgatctt gatgcgcccg accgcattga agccgaggaa agcattgacg
      961 cgcccgatca cctcgctcgt ctcatgctgc aaatgcagcg ccgccatgcc ttcgcaggca
     1021 atgaccagca ccgccggctc aaacgggtcg tcctcatgca tccggcgcgg ccactggatt
     1081 ttctccggcc gagaatggcg gctaaggcgc ggcccggcaa tctcgtccca ggactgcacg
     1141 aggccgatgg agatgccggc gcgcttgcgc agcaccgggt caaggatgcg ggcggtgagg
     1201 tcgcttaccg caacgggatt gcggtccggc tttttccctg ccatgcgttg ttcaaatccc
     1261 atccgaaaag gccagtttcc cgatcaatgg caccgcagcc ccagacccgc aaggccaagc
     1321 ccagcaatga gggtccggtc gctgcacgcc tgctcgactg gtatgacgcg caccaccgcg
     1381 aactgccgtg gcgggtgccg ccgcgcgatc tggcgcacgg catccggccc gatccctatc
     1441 gcatatggct ctccgaagtc atgcttcaac aaaccggcgt ggaaacggta aaggcctatt
     1501 tttccgcctt tgtcgccaaa tggccgactg tcgaggcgct ggcggcggcg acaagcgaag
     1561 atgtgatgaa ggcctgggcc gggcttggct attattcgcg cgcccgcaac ctgaaagcct
     1621 gcgccgatct ggtggcggct tcgggcggca agtttcccga tatagaggac ggcctgcgcg
     1681 ctttgcccgg catcggcgcc tatacggcgg cggccgtcgc tgccatcgcc ttcgaccggc
     1741 cggcgaccgt ggtggacggc aatatagagc gcgtggtttc ccggctgttt tccatcgcaa
     1801 cgccgctgcc gcaggccaag gtcgaaatcc gtgaacgcgt cgggcagctt acgccggaag
     1861 cccggcccgg cgatttcgcc caggcgatga tggatctcgg cgcgaccatc tgcacgccgc
     1921 gcaagccgcg ctgcatgctg tgtccgctgc gcgaggactg cgacgccctg gcatccggtg
     1981 atccggaatt ctttcccgtg cgagcgtcca agccggacaa gccggcacga cgcggcgcgg
     2041 cattcgtggc ggtgcgaggc gatggcgcga tcctgctgcg caagcgtccc gacaagggac
     2101 tgctcggtgg catgagcgag gtgccgacga ccggctggac cgcgcgaacg gatggcgaaa
     2161 cgacgccgaa tgccgcgcct tttgcgggag attggaagcc gatgggcagc atcggccatg
     2221 tcttcacgca tttttcattg gagctggagg ttttccgagc cgacgccgat attgccgccc
     2281 cggccgacgc tttctggtcg ctgcccggcg acattgccgg ggaagcattg ccgacggtca
     2341 tgaaaaaggc catcgaagcg gccattcccg gcgcgacgaa aaaaccgcga ccacagtgaa
     2401 gaggctgcca tgactgaaat ccgccacatc gttttcgata tcggcaaggt gctgatccat
     2461 tacgatccca acattccgtt cagcaggctg atcccggatg aggcggagcg gaagtggttt
     2521 ttcgagaatg tctgcacgca tgaatggaac atcgagcagg atcgcgggcg gacatgggcg
     2581 gaggccgagg cgcatgcgat tgccgcgcat cccggccatg ccgacaacat ccgcaatttc
     2641 cgccgccact ggagcgccat ggtgccgcac gcctatgagg acagcgtggc gctgatggac
     2701 gggctgatcg acgccggcca cgacgtgacc atgctgacca atttcgccgc cgacaccttt
     2761 gtcgaagcgc gccggcggtt cccgttcctg catcgcccac gcggggtgac ggtgtccggc
     2821 gaaatccgcc agatcaagcc cgaccgcgcc atctatgacc atcacaccgc gtcgttcagc
     2881 ctcgatccgg cggcaaccct gttcatcgac gacagccagc ccaatgtcga cggcgcgaag
     2941 gccgccggct ggcaggcggt gctgttcacc ggcgccgaca agctgaagtc ggacctcaag
     3001 acctacggaa tcgccgcatg atttgaatcg cttggcggta gcggttcaga tttcgattca
     3061 acctggcgag cgcggcggcc gcgccgcccc ccacgacacg gctttcggcg gcaggcgaat
     3121 tgctcaccct gcccgttcca tgccgaggcg tgcgatgcgg cgaagctcgt cgatgggagt
     3181 gaggccgcca tttcgctcat aatgccagaa cgtccagccg ttgcaggcgt ccagcccctg
     3241 cacctgcgcg cccatccggt ggatggagcc ggcgctgtcg ccgatggcga ccgtgccgtc
     3301 tgcccgcacc ttggcggcga agcgcttgcg cgaatcgtag agcgtggcgc ccggcgtcat
     3361 caggccagta tcgagaaggc tgacaaaggc gacacgcggc tccgcgcgct tgcccgtcag
     3421 caccgtcaga tcggcaccat gaagcgggac aaccgcgtcg atgcgttcct tggcggcatc
     3481 gatataggcc tgctcgcgct cgatgccaac gaaatggcgg ccaagacgcc tggctaccgc
     3541 gccggtcgtg cccgagccga agaacgggtc cagcacgatg tcgcccggct tggtcgaagc
     3601 catcatgatg cgggcaagca gcgcctccgg cttttgcgtc gggtgcagct tgtcgccatt
     3661 gtcgttcttc agccgctcgc caccggtgca gatcgggaac agccagtcgg aacgcatctg
     3721 gatatcgtcg ttcgacgcct tgagcgcttc atagttgaag gtgtagccct tgcccttctg
     3781 gtcgcgcgat gcccagatca tcgtttcgtg cgcgttctgg aaacggcggc cacggaaatt
     3841 cggcatcggg ttggtcttgc gccagacgat gtcgttcagg atccagaagc cgagatcctg
     3901 gagctttgcg ccgacgcgga aaatattgtg gtaggagccg atgacccaga tggtgccgtt
     3961 cggcttcagc acgcggcgcg ccgccagaag ccatgcgcgg gtgaaggcgt cgtaagcctc
     4021 gaagctttcg aactggtccc aggcgtcgtc caccgcgtcg accttggact ggtccgggcg
     4081 gtgcaggtcg ccgtcaagct ggaggttgta gggcggatcg gcgaagatca cgtcgatgga
     4141 tttttcaggc aaacgctcta gcgctgcgac gcaatcgccc ttgagaatgg tatccagcca
     4201 atcggactgc tgcgaagcat ggggaaggtc ggcgagaaga cgcacggcag acatttttac
     4261 acccgaggca cacggttact gtttacgcct cgttatggtt accttttagc gtaaatattt
     4321 agtgaagtgc cacatggggg cgtggcgaat ttgccgccgc gcccggcttg gtgtatgccg
     4381 cctgcgcccg gttcgaccgg ttcttccccc tctcgccagt ggtttgccga tgcctttgcc
     4441 agaacttgtc attttcgatt gcgacggcgt gctcgtcgat tccgagatca ttgcggcgcg
     4501 cgtcgagtcc gaactgctga cggcttccgg cttcgagatt tcagccgagg agcttgccga
     4561 gacctatgcc ggcctgacct tcaaggacat tctgatcaag gtggaggaaa tctcgaaaat
     4621 accgttccag atatcgctga tcgaccgtgc cgaggaactg atcgaccgca agctgcgcac
     4681 cgacgtgcgc gccatcgagg gcgtgcatga ggcggtggcc gcggtgccgg tgccattttg
     4741 catctgctcg aactcgcgca cggagcggat cgaactgatg ctggaaaagg tgcggctgct
     4801 gccactgttt tccggccgca tcttttcggc gcttgaaacg ccgacgggca agccgaagcc
     4861 cgcgccggac gtgttcctcc atgccgccga aaagcttggc gccgacccgg cgcgctgctt
     4921 cgtcgtcgag gattccgtgc atggcgtcgc cggcgcggtg gccgcgggca tgcgggtgat
     4981 cggcttcacc ggcgcgtcgc acagctatcc cggccacgcc gacgcgctga ccgaagccgg
     5041 cgcggaaacc gtcatccggc gctggccgga gctgaaaagc gtgatcgcgg cgctttccga
     5101 atggtcggat gcctaagata ttggacgcct gagagccgga cgccttattt cttcttcttg
     5161 gcgggcgggc cttcgtcgta accggcgaag acctgatagt tcttcgaaga cggctcaggc
     5221 acgctcacat tgggatcgct gaagacgaac tgggtggcgg ccgacatgtc ggtcacctgg
     5281 agcttgtact ggtgaagctg tgagtaaatg acctgcgatc cctccaatac ggcgatgcgg
     5341 atcggcatgg tgatgctgcc ggcggtcgcc ttcgggccag gaacgacctt gccggcgacg
     5401 gcgacattca tggtcagtgt gccggcattg cgggcgcagt cgcgcgttac gtcggtaatc
     5461 gccgcctggt agacgatgtt ggccgactgg tcctcggcat cggcggcggg cgcggcatcc
     5521 gcctcggcat cgtccgcctt cttcaccttt ttcttggcgc cgttggcgta ggtgttgaaa
     5581 taggcggtgc cgtcgcgcag cgtgattgtg gggcaatagg ccatcagatc gctggccttg
     5641 accctggtat cctcgacctt cgcctgctgg ctgccgcctc cgccgatgcc aagcacaccc
     5701 ttgtcgccag attggcaacc cgcgacggcg agcataaagc ccgacaacgc caaacccgcg
     5761 aaaaagcgag ccttaaaaga acgaaacacc atgaactgca cttccctctc gtaaagttgg
     5821 tgtcctatag caagccaacg gcaaaaatgc gaccggcgaa gcaggcaaat ccaccactgt
     5881 gccgccggcc cgaacggcgg atagcaacgg acggctgaca atgcactatg tgagcacgcg
     5941 cggggaagca cccgttttgg ggttttccga tacgataatg gctgggctgg cgcgcgatgg
     6001 cggcctttac gtgccgcgcg aatggccgca tttctcgacc gctgaaattc gcgccatgcg
     6061 cggcctttcc tatcccgacc tcgccattcg cgtgctgacg ccgttcctcg gcggcgagat
     6121 cgcggcgccg gtgttcgaaa ggctggtgcg cgaggcctat gcgacctttc gccacgaggc
     6181 cacctgcccg ctggtgcagc tcggcaccaa cagcttcgtg ctggagctgt tccacggacc
     6241 gacgctggcc ttcaaggacg tggcgatgca attgctggcg cggctgatgg accatgtgct
     6301 ggccgagcgc ggccagcacg ccaccatcgt cggcgcaact tcgggcgaca ccggcggcgc
     6361 ggccatcgac gcttttgccg ggcgcgagcg caccgacatc ttcatcctgt tcccgcatgg
     6421 caaggtctcg ccggtgcagc agcggcagat gacgacatcg tccgccgcga atgtccatgc
     6481 gctggccgtc gaagggaatt tcgacgactg ccaaagcctg gtcaaaggca tgttcaacga
     6541 tcatgcgttt cgcgaccggg tggcgctttc gggcgtcaat tcgatcaact gggcgcgcat
     6601 catggcccag atcgtctatt atttttcttc cgcgctttcg cttggaagcc ccgaccgacc
     6661 catatctttt acggtgccga ccggcaattt cggcgatatt ttcgccggtt acgcggcaaa
     6721 gaagatggga ctgccgattg aacggctggt gattgccacc aacgacaacg acattctggc
     6781 gcgcacgctg gcaagcggcg aataccggac gaagggcgtg atttccacca cctctccctc
     6841 catggacatc caggtatcct ccaatttcga acggcttttg ttcgaggctt ccaaccgcga
     6901 ttcgggcaag gtgcggcgct acatggacgg gctgaaacag tcgggcgctt tcaccataga
     6961 ggccgatggg ctggccggta tccgcgctga attcgacgcc gggcgcgcga cgatggagga
     7021 gacggcggca acgatccgct cgacgctcga aggctcgaac tacctgctcg atccgcacac
     7081 ggccgccgcc gttcacgtcg cggcaaggcg caccggcgag gtgccgatgg tggtgctggg
     7141 caccgcgcat ccggcaaaat ttcccgccgc cgtcgaggcc gcgagcggcg tgtcgcccgc
     7201 cctgccttca tggctgtcgg gcttgatgag cgcagaagaa aaattctcgg tacttccatc
     7261 tgacctgaaa atgttggaag attacgtcgg ccgccacacg cgggcggcgc gttagggagt
     7321 ctattttata tgggtgtaga ggtaagccgt ctgtcgaacg gcctgacagt cgccaccgaa
     7381 acccttccaa gcattgaatc cgtcgctcta ggtgtctgga tcaaatccgg cgcccgcaac
     7441 gaacgtgacg aagagcacgg aatggcccac ctgcttgaac acatggcgtt caagggaacc
     7501 aagcggcgca ccgcattcga gatcgcttcc gagatcgaga atgtcggcgg cgagatcaat
     7561 gcggcgacca gcgtcgagac gacctcctat ttcgcgcggg tgctgagcgc cgatgttccg
     7621 cttgccgtcg atattctcgc cgacatcctg caagaatcgg aattcgatcc gcatgagttg
     7681 gagcgcgaac agcacgtcat cctccaggaa ataggcgctg cccacgacac gcccgacgat
     7741 gtcgtcttcg accatttcac cgagacggcc tttcgccacc agacgcttgg ccgttccatt
     7801 ctcggcacgc cggaaacggt gaaatccttc acctccacgc aactccacga ttatatcggc
     7861 cgccagtatg gcgccgagcg gatggtcgtg gtggccgccg gcgacgtcaa gcatgacgat
     7921 ttcgtgcgcg aagtggaaaa gcggcttggc ggctttcgca gcaaggccga aagcatcatg
     7981 ccgcaatatg cgcaatatgt cggcggcgac ttccgcgaga accgcgacct gatggatgcg
     8041 cagatcgtgc tgggcttcga gggccgcgcc tatcatgtgc gcgatttcta cgcctcgcag
     8101 gtgctgtcga tgattctcgg cggcggcatg tcttcgcgcc tgttccagga agtgcgcgaa
     8161 aagcgcggcc tgtgctattc ggtctatgcc tttcactggg gtttttccga taccggcatc
     8221 ttcggcgtcc atgccgccac cggccagtcg agcctgggcg aactggtgcc ggtcatcatg
     8281 aacgagttgc agcgaaccgg cgaaaacatc ctgcaagaag agcttgaccg cgcccgcgcg
     8341 caataccgcg cagggcttat catgtcggcc gaaagcccct ccagccgcgc atcgcagatt
     8401 gcccgccaat tgctgctgtt tggccggccc gtcgccaagg aagagctgat ggagcggctg
     8461 gacgcgctga cgaccggacg gttgacggac ctgtccggcc ggctgttttc gacccgcccg
     8521 acgcttaccg ccgtcggtcc ggtgggcact ctggcgccct acgaagaact gcttgatctg
     8581 gtttcgggtt cgcagacggc ggcccgcaaa ctcgccgtct gatccagaac ggtgttgtcc
     8641 ttccttcgcc gcgaatcacc ggcactgaag ggcaagcagg tcatgctgcg ggttccgtca
     8701 cccggcgact accgggaatg gtcgacgttg cgcggcgaga gccgcggctt cctcgaaccg
     8761 tgggagccgc gctggacgcc ggaagaactc gaacgccacg cctggcgcca ccgcatccac
     8821 cgctaccgcg aagactacgc gctcggcctg tcgatggcgt ttttcatctt cgaacttggc
     8881 agcggcaaac tggtcggcgg cataacgctt ggcaatatcc gccatggcgt ttcgcagagc
     8941 ggccatgtcg gctactggat cggcgagcgc tatgcggggc gcggcctgat gaccgaggcg
     9001 gtcaacgtgc tggcgcagtt cgctttcgat acgctgaggt tgcaccggat cgaagctgcc
     9061 tgtattcccg acaacgcccg ttcgatccgt gtgcttgaaa aagccggatt ccggcgcgaa
     9121 gggctgttgc gatcctacct gcggatcaat ggcttctggc aggaccatta tctctacgcc
     9181 cgtatcgccg atgacgggcc gggcgagcga acaaagggct gatttttgac gaactcgccg
     9241 cgcatccgat cgcttctggc gtttgtcctg gcggcaatcg tcactttctg ttcggtcacc
     9301 gcgacattcg cggtcgagcc tatcaagatc gcgcgcgacg atgtggcgct cgatctttcc
     9361 ggcgccgtcg agatttaccg caaccagggc gaaaacttcc aggtttccac cgcgcccggc
     9421 cccgacggca tcgtgcgccg catcgaggtg gaggccaacg acaagcgctc gaccggcgac
     9481 tgggcggtgt tcgcgctggc caacacgacc gaccagcagc tcgaccggct gatcgtcgcg
     9541 ccgcattttc gcctggtcaa ttccggcatc ttctggcccg atctcggctc gacgcgcatc
     9601 gccgcgatca cgcccagcga aggcttcgcg cttgaccgcc agaccagccc ggatgccgac
     9661 gtgtttcgcg tcacgctcaa tccgggcacc gtcgtcacct tcattgccga gcttgcctcg
     9721 ccgaaactgc cgcaggtcta tctgtgggag ccggaatcct acaaggatac cgtcaattcc
     9781 tacacgctct atcgcggcat cgtcatcggc atcgccggcc ttttggcgct gttcctgacc
     9841 atcctgttcg tggtcaaggg cacctcgatg ttcccggcca ccgcagcgct tgcctgggcg
     9901 gtgctggcct atatctgcat cgatttcggc tttctcaaca aggtcatcga aatatcgccc
     9961 ggcagcgaac agatgtggcg ggcgagcacg gaagtgggcc tcgccaccac cttcgtggtg
    10021 ttcctgttca cctatctcaa cctcaaccga tggcacggcc atttcagcta tggcgcgctg
    10081 gcctgggtcg ccgggctggt gctgatcgcc ggcgtcgcca tcatcgatcc ggccatcgcc
    10141 gccggcatcg cccgcatctc gtttgcggca accgccatca tcggcctgtg cctcatcgtc
    10201 tatctcggca tacgcggcta cgaccgcgcc atcatgctgg tgccaagctg ggtgatggtg
    10261 cttttgtggc ttgccggctc gtggatggcg atcaccggcg ttctggacaa cgacattgcc
    10321 cagcccgcac ttggcggcgg cctcatcctc atcatcctgt tgatcggctt caccgtcatg
    10381 cagcatgcct ttgccggcgg cgcgctgcat caggggctgt tttccgacct tgagcggcag
    10441 gcgatggcca ttatcggctc cggcgatacg gtgtgggact gggacgtgct gcgcgaccgc
    10501 gtcgtcaccc ggcccgacat cagcctgcaa ctcggcctcg aaccgcatag cctgagcggg
    10561 gctgcgcgca actggctgcc cgtgctgcat gccgacgacc gcgacacgtt ccgcaccacg
    10621 ctcgatgtcg tgctggagca ccggcgcggc aaggtggcgc agaatttccg cctgcgcggg
    10681 gcggacggcc actaccactg gttcgcgctc cgggcgcgcc cggtgatcgg ctcggacggc
    10741 caggtcatcc gctgcgtcgg caccatgatc gacgtgaccg agcagaagaa atccgaagag
    10801 cggctgctgc acgacgccgt gcacgacaat ttgaccggcc tgcccaaccg cgaactgctg
    10861 atgaaccggc tggagacgat catctcgatc gcccgcaccg aggagaaagt tcgcccgacc
    10921 gtcttcgtca tcgacatcga ccgcttcaag caggtcaatg acgggcttgg catttcggcc
    10981 ggcgacacca ttttgctgac ggtggcgcgc cgccttcacc gcctgctcat gccgaaggat
    11041 tcgctgtcgc gctttgccgg cgaccagttc gcgctgatgc ttttgtcgga acaggagccg
    11101 ggccgcatcg ccgccgtcgc cgacgccatc aagcacgcga tcaatgcgcc gatcaccttc
    11161 gccaagcgcg agatcgtgct gaccgcctcc atcggcctga taagctggac ctcggaacaa
    11221 acctcggcgg aagacatggt caaggacgcc gagcttgcca tgcatcaggc gaaacgcttc
    11281 ggcggcgacc gcatcgagcc gttccgcccg gccttccgca ccgtcggctc ggaccggctg
    11341 caattcgaat cggacctgcg ccgcgcggtg gagcgcaggg aattcacgct ggcctaccag
    11401 cccatcgtgc ggctggaaga caacagcatc gccggtttcg aggcgctttt gcgctgggac
    11461 catccccgcc gcggcatgat accgccggcg gatttcattc cggtggcgga aagctgcggc
    11521 ctcatcgtgc agctgggcct gtttgccatg cagaaggccg ccgaagattt ggccgcgtgg
    11581 caaaagcaga tcagcgacgc gccgctttcg gtttccgtca acctttccag ccgccacctc
    11641 atccgccgcg acctggtcag cgacgtgcga tcggtcattg cgcgcgccaa tttgaagccg
    11701 cgctgcttcc ggctcgaact gaccgaatcg ctggtgatgg acaatcccga gcaggcggtc
    11761 catgtgctca acaagctcaa gcagcttggc gtcggcctgt cgctcgacga tttcggcacc
    11821 ggctattcgt cgctgtccta tctgacgcgc ttccccttcg atacgatcaa gatcgacaag
    11881 agcttcgtcg acgacaatac gccgaagcgc tcggtgctgc tgcgctcaat ggtcaacatg
    11941 gcgcatgagc ttggcctgtc ggtggtggta gagggcattt ccgaccagaa ggatgcgctg
    12001 gagttgcgtc agatggggtg cgaatatgtg cagagcttca tgttcgggcc gccggtcgcc
    12061 ggcgacatcg tgctgaagat gctgaaagag caatatccgc tgacgcaggc atgatcggca
    12121 cgaaagcctt gctctcgcgc tgattcaccg aagcaaccca actatttgaa tataaagact
    12181 tcccgccgat tccatcaggc gtggccggcc gcgccgctca gatgcgccag gtcgatgccg
    12241 attgaggcaa gcgccttgtc gtatttgcgg tcgatgtcgg catcgaacag aagctcgggc
    12301 gtggccggac aggtcagcca gccgtttccg gcgatctcgc tttccagttg cccggcgccc
    12361 cagcccgcgt aaccaagcgc catcagcgcc tggcgcggac cgcgcccgct ggagatggcg
    12421 cgcagaatat cgacggtcgc cgtcaggcag atatcatccg agacggccag cgaggattcc
    12481 accttatagt cgcccgaatg cagcacgaag cctcggctgc ggtcgaccgg accgccattg
    12541 cggacgacga aatcgcgcgc cggcgcgggc aaccggatcg cttcctgctc gtccaatatg
    12601 ccaagctgca ccagcaaatc cgggaacagc atctgctgcg tctggttgac gatcagcccc
    12661 attgcgccct cttcgctgtg ggcgcagaca taaataacgg cgcgcgcgaa ccggtcgtcc
    12721 ttcatgccgg gcat
//
LOCUS       DASYCS010000327         1547 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2986791,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000327 DASYCS010000000
VERSION     DASYCS010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2986791"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VGM46_10270"
     CDS             <1..367
                     /locus_tag="VGM46_10270"
                     /inference="COORDINATES: protein motif:HMM:NF044545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193018.1"
                     /translation="FNQVVTAPFAGATHRERALIATAVFHRYSGDEDFPADISAHDLL
                     GEEDERLARRIGLAARLAFSVSASAERELPHYPLRMTPTRLLLDVSSRRQRIAGEPVQ
                     KRLGTLAAIFDRKGEILIG"
     gene            466..681
                     /gene="tatA"
                     /locus_tag="VGM46_10275"
     CDS             466..681
                     /gene="tatA"
                     /locus_tag="VGM46_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEY4193019.1"
                     /translation="MGTLSIWHILILVIVALLLFGGRGKLSDLMGDMAKGITSFRKGL
                     QDPDQDDHRDDRRPPISVDRDKDQVGR"
     gene            697..1089
                     /gene="tatB"
                     /locus_tag="VGM46_10280"
     CDS             697..1089
                     /gene="tatB"
                     /locus_tag="VGM46_10280"
                     /inference="COORDINATES: protein motif:HMM:TIGR01410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HEY4193020.1"
                     /translation="MFDLSWSHILIFLVVALVVVGPKDMPRLLRIAGQWVGKARGMAN
                     EFRKSFDEMARASELDELRQEIENLRRERPLSEFENEMNKPLEPAPMMRPLDTPLDPG
                     PAMDVYNHTVEEVPPEVAEPPVAQEAKQ"
     gene            1086..>1547
                     /locus_tag="VGM46_10285"
     CDS             1086..>1547
                     /locus_tag="VGM46_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006466666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEY4193021.1"
                     /translation="MSDRTADDEAHIEASKAPLMEHLVELRRRVIWSLITFVLCFVFC
                     FAFAKYIYAFLTWPLANALRGQPNDHLIYTALYETFFTYVKVGMFGGLCLAFPVVATQ
                     LYLFVAPGLYKNERKAFLPFIIASPLLFILGGSFVFFVMLPFAIKFFLGYQT"
BASE COUNT          287 a          500 c          446 g          314 t
ORIGIN      
        1 tttcaaccag gtggtcaccg cgcccttcgc cggcgccacc caccgcgagc gcgcgctcat
       61 cgccaccgcc gtcttccacc gctattcggg cgacgaggat ttccccgccg atatcagcgc
      121 gcacgacctt ctgggcgagg aagacgaacg gctcgcccgc cgtatcggtc tggcggcgcg
      181 gctggccttt tcggtctccg cctctgccga gcgcgaactg ccgcactacc ccttgcggat
      241 gaccccgacc cgcctgctgc tggacgtttc cagccgccgc cagcggattg cgggcgaacc
      301 ggtccagaaa cgattgggaa cgctggccgc gattttcgat cgaaagggcg agattttgat
      361 cggctaaggc gcgagtaagg tcttgtcgcc gcagggcgaa ggcgctttaa ccccgcgcaa
      421 ggcatcggca tcctatataa gagccggacg ccgcaggaga acaccatggg cacgctcagc
      481 atctggcaca ttcttatcct tgtgatcgtg gccctgcttc tgttcggtgg ccgcggcaag
      541 ctttccgacc tgatgggcga tatggccaag ggcatcacca gtttccgcaa gggtctgcag
      601 gaccccgacc aggacgatca tcgcgacgat cgccgcccgc cgatttcagt ggatcgcgac
      661 aaggatcagg tcgggcgtta atcccagcca agtccgatgt tcgatctaag ctggagccat
      721 atccttatct ttctggtcgt ggccctggtt gtggtcgggc cgaaggatat gccccgcctg
      781 ctgcgcatcg ccggtcaatg ggtcggcaag gcgcgcggca tggcgaacga attccgcaaa
      841 agtttcgacg agatggcgcg cgcatccgaa ctggatgagt tgcgccagga aatcgaaaac
      901 ctgcgccgcg aacgtccgct ctccgaattc gaaaacgaaa tgaacaagcc gcttgagccc
      961 gcgccgatga tgcgcccgct cgacacaccg ctcgatcctg gcccggcgat ggatgtttac
     1021 aatcacaccg tcgaggaagt gccgccggaa gttgccgaac cacctgtcgc gcaagaagcc
     1081 aaacaatgag cgaccgcacc gcggacgacg aagcccatat cgaagcgagc aaggcgccgc
     1141 tgatggagca tctcgtcgaa ctgcgccggc gcgtcatctg gtcgctgatc accttcgtgc
     1201 tctgctttgt tttctgtttc gccttcgcca aatatatcta cgccttcctg acatggccgc
     1261 tggcgaatgc gctgcgcggc cagcccaacg atcatctgat ctacaccgcg ctgtacgaaa
     1321 ccttcttcac ctatgtgaag gtcggcatgt tcggcgggct gtgcctcgca ttcccggtgg
     1381 tggcgacgca gctttatctt ttcgtagcgc ccggcctcta caagaacgaa cgcaaggcgt
     1441 tcctgccctt catcatcgca tcgccgctgt tgttcattct gggcggatcg ttcgtgtttt
     1501 tcgtgatgct gcctttcgcg atcaaattct tcctcggcta ccagacg
//
LOCUS       DASYCS010000328         2166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1396712,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000328 DASYCS010000000
VERSION     DASYCS010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1396712"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1383)
                     /locus_tag="VGM46_10290"
     CDS             complement(<1..1383)
                     /locus_tag="VGM46_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018426834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003682 - chromatin binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007076 - mitotic chromosome condensation
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation SMC family protein"
                     /protein_id="HEY4193022.1"
                     /translation="MKFSRLRLLGFKSFVEPGEFVIERGLTGVVGPNGCGKSNLVEAL
                     RWVMGESSYKNMRASGMDDVIFSGSGTRPARNTAEVTLFLDNSSRTAPAAFNDADELQ
                     VSRRIERDAGSLYRINGKEARAKDVQLLFADQSTGARSPSMVGQGRIGELIQAKPQAR
                     RALLEEAAGISGLHTRRHEAELRLKGAEQNLERLDDVVGQLESQVESLKRQSRQASRF
                     KSLSADIRKADAMLLHLRWTLAKAQEGEAHSALAVATALVGDRAAAQMNTAREQGIGA
                     HHMPELREAEAAAAAAFQRLSIARTQIEEEAGRISQRQAELDRRLQQLDGDIVREERM
                     VRDNADILERLAGEEQALAAENAGAAEREANTRAAFEQAATTLELSEAKLTALTAEKA
                     EAAASRTQIERALRETAERRDRFGRQMAEIDRELSDIAARIAGLADPAQKKLLVEQAE
                     AVLEETEAAAE"
     gene            complement(1441..2166)
                     /locus_tag="VGM46_10295"
                     /pseudo
     CDS             complement(1441..2166)
                     /locus_tag="VGM46_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006330664.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
BASE COUNT          343 a          720 c          687 g          416 t
ORIGIN      
        1 ttcggccgcc gcttcggttt cttccagcac cgcctcggcc tgctcgacca gcagcttttt
       61 ctgcgccggg tcggccaagc cggcgatacg cgcggcaata tcggagagtt cgcggtcgat
      121 ttcggccatc tgccggccga aacggtcacg ccgctcggcg gtttcacgca gcgcccgttc
      181 gatctgcgtg cgggaagcgg cggcttccgc tttctcggcg gtcaaggccg taagcttcgc
      241 ctcgctcaat tccagcgtcg tcgccgcctg ttcgaaggcg gcgcgggtgt tggcctcgcg
      301 ctcggcggcg ccggcgttct cggccgccag cgcctgttcc tcgccggcca aacgctccag
      361 aatatcggca ttgtcgcgaa ccatgcgctc ctcgcgcacg atatcaccgt ccagttgctg
      421 caagcgacgg tcaagctcgg cctgacgctg gctgatgcgc ccggcttcct cctcgatctg
      481 cgtcctggcg atcgacaggc gctggaaggc ggcggcggcg gcggcttccg cctcgcgcaa
      541 ttccggcatg tggtgcgcgc cgatgccctg ttccctcgcc gtattcattt gcgcggcggc
      601 acggtcgcca accagcgcgg tcgccaccgc cagcgcggaa tgggcttcgc cttcctgggc
      661 ctttgccagc gtccagcgca gatgcagcag catcgcatcg gccttgcgga tatccgccga
      721 caggctcttg aagcgggagg cctggcgcga ctggcgcttc aggctctcga cctggctttc
      781 caactggccg acgacatcgt ccagccgttc cagattctgc tccgcgccct tcagccgcaa
      841 ctcggcctcg tggcggcgcg tatgcaggcc ggaaatgccg gcggcttctt ccaaaagcgc
      901 gcggcgcgcc tgtggcttgg cctggatcag ctcgccgata cggccctggc cgaccatgga
      961 aggcgaacgg gcgccggtgg actggtcggc aaacagaagc tgcacgtcct tggcgcgcgc
     1021 ctccttgccg ttgatgcggt agagcgagcc ggcatcgcgc tcgatgcggc gcgaaacctg
     1081 caattcgtcg gcgtcgttga aggcggcggg cgcggtgcgg ctcgaattgt cgagaaaaag
     1141 cgtgacttca gcggtgttgc gggcgggccg cgtgcccgac cccgaaaaga tcacgtcgtc
     1201 catgccggac gcgcgcatgt tcttgtagga gctttcgccc atcacccagc gcagggcctc
     1261 gacaaggttg gacttgccgc agccattcgg cccgacgacg cccgtcaggc cgcgctcgat
     1321 gacgaattcg ccgggttcaa cgaaggattt gaaaccgagg aggcgcaggc gcgaaaactt
     1381 catgcgcgcg ccgccgcaaa cgcgcgcgcg ccatcgcgcg ctcggcttcc tgccgagact
     1441 ttaaagcagc gggtcgatga ccgccgagat ttcgtcaatc gacagtgccc ctttgtaggt
     1501 ctgaccgttg atgaagaagg tgggcgtcga gtccaccttg aactcatcgg ctccgcgctt
     1561 ctgcaccgct ctcacttggt ccagaagttt ctggtccgtc aagcatttgt cgaagctctc
     1621 ctgtgtaaaa ccggcgagct tggaaagctg caacagcgca tccttgacgt tatcgacggc
     1681 cacccagttc tgctgctgct tgaacagcac gtcgaccatc gggaaatagt tgtcgcccgc
     1741 gcagcgcgcc agcatgaagc cggcttcggc gcgcggatcg aacgggaatt cgcgcaggat
     1801 gtagcgcacc ttgccggtgt cgatatattt cgtcttcatt tccggaaagg tggtctcggc
     1861 gaaatgctcg caatgcgggc acgtcatcga cgcatattcg acgaccgtga ccttggcatc
     1921 atccttgccc agggactttt caggcagggc acccggcttc agcaattcgg ccatgtcgac
     1981 ggaaccggac gattccggcg cggcgacggc cggcttcatg gcgtcggccg gcttcatggc
     2041 attggtggcg ggcttcatcg cgtcagccgg ctacatggcg ttgccgggct tgacctcggc
     2101 tgccttggct tcctcgcctg aatcactaca ggcagcgagc ataaccaccg ccgacacgga
     2161 ggccag
//
LOCUS       DASYCS010000329         3178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2121507,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000329 DASYCS010000000
VERSION     DASYCS010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2121507"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /gene="moaA"
                     /locus_tag="VGM46_10300"
     CDS             <1..727
                     /gene="moaA"
                     /locus_tag="VGM46_10300"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019008 - molybdopterin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0061798 - GTP 3',8'-cyclase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEY4193023.1"
                     /translation="ALEELTLTTNGSQLSRFAAELADCGVKRINVSLDTLDPAKFHQI
                     TRWGHLEKVMEGIDAAQAAGLKLKINAVALKGFNEAELPEMMRWAHGRGIDLTVIETM
                     PMGEIDEDRTDRYLPLSLLRASLERQFTLTDIPYKTGGPARYVEVAETGGRMGFITPL
                     THNFCESCNRVRLTCTGTLYMCLGQDDAADLRAPLRASEGNDLLSAAVDEAIGRKPKG
                     HDFVIDRHTRRPSVSRHMSVTGG"
     gene            841..1761
                     /locus_tag="VGM46_10305"
     CDS             841..1761
                     /locus_tag="VGM46_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY4193024.1"
                     /translation="MPLSPNLRGAAFMVASMVGFTISDSMTKYLSESMNIAQVMLVRG
                     LFATTFIALLAWSRGALATPSLMLHPMVAVRVAGEASATVAYFIALSHLPIANVSAVQ
                     QALPLAVTMGAALLFGEYVGPRRWLAIAAGLAGVLIIVRPGFDGFNAYSLLVLLCVVL
                     CAIRDLATKQVPDSIPTLLVSVATSIVVTLVGAAMIQPMGGWSPVSAKHLALLVSGAV
                     LLLIGYQFIIMAMRIGDISFIAPFRYTALLCAILLGVLVFGDFPDMPMIIGATIIVCS
                     GIYTLYRERIVGRGRAAAESTGPAMAPDGI"
     gene            1798..2427
                     /gene="mobA"
                     /locus_tag="VGM46_10310"
     CDS             1798..2427
                     /gene="mobA"
                     /locus_tag="VGM46_10310"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HEY4193025.1"
                     /translation="MNHEIAGIVLAGGLSTRMGGGDKGLLALAGRPVLAHVISRLEPQ
                     LGSLALNANGDPSRFETFGLPIIADTVAGFAGPLAGVLAGLEWAASATGAKFVVSAAG
                     DTPFLPADLVGKLSSALAGQAGQVSVACSGGRRHPVFALWPVSLRSTLADVLRRGERK
                     VSALQDMLGCIEVQFPIEQWGARRVDPFFNINTPQDLADAEPMTGSRKS"
     gene            2424..2933
                     /gene="mobB"
                     /locus_tag="VGM46_10315"
     CDS             2424..2933
                     /gene="mobB"
                     /locus_tag="VGM46_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HEY4193026.1"
                     /translation="MNRRVFGITGWKNSGKTTLTEKLVTALTARSRRVSTVKHAHHDF
                     DIDKEGADSFRHRQAGAVEVAIVSGRRWALMHELRDEQEPSLDTILARLGDADIVLVE
                     GYKREAHKKIEARRLGAKDNAPLSPNDPNIVAVASDHAQPGEMLPVFDLDDIEAIADF
                     IERITGLTS"
     gene            3082..>3178
                     /locus_tag="VGM46_10320"
     CDS             3082..>3178
                     /locus_tag="VGM46_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter"
                     /protein_id="HEY4193027.1"
                     /translation="MRFALRIALAASAALLTLGVAQAQEKTIKIGT"
BASE COUNT          558 a          994 c          980 g          646 t
ORIGIN      
        1 cgcactggaa gaactgacgc ttaccaccaa tgggtcgcag ctttcgcgct ttgccgctga
       61 actggccgat tgcggcgtca agcgcatcaa tgtctcgctg gatacgctcg accctgccaa
      121 attccaccag atcacccggt ggggccacct cgaaaaggtc atggaaggca tcgatgcggc
      181 gcaggccgct ggcctgaagc tgaagatcaa cgcggttgcg ctgaaaggct tcaacgaagc
      241 cgaactgccc gaaatgatgc gctgggcgca tgggcgcggc atcgacctga cggtgattga
      301 aaccatgccg atgggcgaaa tcgacgaaga ccgcaccgac cgctatttgc cgctttcttt
      361 gctgcgcgct tcgctggagc gtcagttcac gctgaccgac ataccctaca agaccggcgg
      421 acccgcccgt tatgtggagg tggccgagac cggcgggcgc atgggcttca tcacgccgct
      481 gacgcataat ttctgcgaaa gctgcaaccg tgtccgcctc acctgcactg gcacgctcta
      541 tatgtgcctc ggccaggacg acgctgccga cctgcgcgcg ccgttgcggg cgtcggaagg
      601 caacgacctt ctttcggcag ccgtcgacga agccatcggc cgcaagccca aggggcatga
      661 tttcgtcatc gacaggcaca cccggcggcc ttcggtatcg cggcatatga gcgtcaccgg
      721 cggctgaaac cggctagccc gtactcagct cggctgtccg gctagtggca tagtgctttg
      781 atcttgctgt ttttctggcg ctggtacagg ctgcgtgcgc aacgaaaccg gaagcacggc
      841 gtgcccctat ctccaaatct tcgcggcgcg gcgttcatgg tcgcatccat ggtcggcttc
      901 acgatcagcg attcgatgac gaaatacctg tccgaatcca tgaatatcgc gcaggtcatg
      961 ctggttcgcg gtctgtttgc cacgacgttt atcgcgctgc tcgcctggtc gcgcggcgcg
     1021 ctggcgacgc caagcctcat gctgcacccg atggtggcgg tcagggtggc tggcgaggcc
     1081 tcggccaccg tcgcctattt catcgcgctg tcccatttgc cgatcgccaa tgtctcggcc
     1141 gtccagcagg cgctgccgct ggcggtcacc atgggcgcgg ccctgctttt cggcgaatat
     1201 gtcgggccac gccgctggct ggctatcgcc gccgggctgg caggcgtcct cattattgtg
     1261 cgtcccggtt tcgacgggtt caacgcctat tcgctgctgg tgctgctctg cgtcgtcttg
     1321 tgcgcgatcc gcgaccttgc gaccaagcag gttcccgatt ccattcccac cttactggtg
     1381 tcggtcgcaa cgtcgatcgt cgtcaccttg gttggtgccg ccatgatcca gccgatgggc
     1441 ggctggtcgc cggtgagcgc taaacacctc gctttgctgg tatcgggcgc ggtgcttctg
     1501 cttatcggct accagttcat catcatggcc atgcgcattg gcgacatctc cttcatcgcg
     1561 ccgttccgct acacggcctt gctgtgcgca attttgcttg gcgtgcttgt cttcggcgac
     1621 tttccggaca tgccgatgat tatcggcgcc acgatcatcg tctgttcggg catctatacg
     1681 ctgtaccggg agcgcatcgt cgggcgcggc agggcggccg ccgaaagcac tggaccggcc
     1741 atggcgccgg acggcatata gccggatacg taccgattcc ggcagatgca cttggcaatg
     1801 aaccacgaaa tcgcaggcat agttctcgcc ggcggccttt ccacgagaat gggcggcggc
     1861 gataaaggcc tgctggccct cgccggccgg cctgttctcg cgcatgtcat ctcgcgcctt
     1921 gaaccgcaac tgggttcgct ggcgctcaac gccaatggcg acccttcccg tttcgagacc
     1981 tttggcttgc cgattattgc cgacacggtc gcaggcttcg ccgggccgct ggccggcgtg
     2041 ctggcgggcc tggaatgggc ggcatccgcc accggcgcga aatttgtggt gagcgcggcc
     2101 ggcgatacgc ctttcctgcc tgccgacctc gtcggcaagc tctccagcgc gctcgccggg
     2161 caggcgggac aagtctctgt cgcctgctcc ggcggcaggc gtcacccggt atttgcgctg
     2221 tggccggttt ctctgcgaag cactctcgcc gatgtccttc gccgaggcga acgcaaggtc
     2281 tccgcccttc aagacatgct cggctgcatc gaagttcaat ttcccatcga acagtggggc
     2341 gcgcgcaggg tggacccgtt cttcaacatc aacaccccac aggatcttgc cgatgcggag
     2401 ccgatgacgg gaagcaggaa atcatgaaca ggcgcgtttt cggcattacc ggctggaaga
     2461 actccggcaa gacgacgctt accgaaaagc tcgtcaccgc gctgacggcg cgcagcaggc
     2521 gcgtctcgac cgtaaagcac gcccatcacg acttcgatat cgacaaggag ggcgcagaca
     2581 gttttcgcca ccgccaggcc ggcgccgtcg aggtggcgat cgtatccggg cggcgttggg
     2641 cgctgatgca cgagttgcgc gacgaacagg agccttcgct ggacacgatt ttggcgaggc
     2701 tgggcgacgc cgatattgtg ctggtcgaag gctacaagcg cgaggcgcac aagaaaatcg
     2761 aagcgcgccg gctgggggcc aaggacaacg cgcctctttc gccaaacgac ccgaatatcg
     2821 ttgccgtcgc ttccgaccat gcccagccgg gcgaaatgct gcctgtattc gatctcgatg
     2881 atatcgaggc gattgccgat ttcatcgagc gcataaccgg tttgacatcc tgattgcacg
     2941 acgaggcgag tttgccggcg ctatttgttt tgcagttgct ttcctgttga aaagaatggg
     3001 agtatctggc cagcgggcgg tcgggaggat cgcccggaag gccgtataaa taacggcttg
     3061 caccatacag agaggaatat tatgcgtttt gcactgcgta tcgcgctggc ggcatctgcc
     3121 gcgctactga cgctcggcgt tgcccaggct caggaaaaaa caatcaagat cggtacgg
//
LOCUS       DASYCS010000330         3578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_90264, whole
            genome shotgun sequence.
ACCESSION   DASYCS010000330 DASYCS010000000
VERSION     DASYCS010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90264"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="VGM46_10325"
     CDS             <1..340
                     /locus_tag="VGM46_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-carnitine dehydrogenase"
                     /protein_id="HEY4193028.1"
                     /translation="MAQFGPCLKWPWTKLMDVPEFNDELVDLIATQSDEQSDKWSIRE
                     LEKIRDDNLVAIMDALSKQNKGKGWGAGALHKDYTRQLAKAAKPAVSKAAAKAKADKP
                     KKKAATKKKG"
     gene            344..1519
                     /locus_tag="VGM46_10330"
     CDS             344..1519
                     /locus_tag="VGM46_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEY4193029.1"
                     /translation="MDFGLSEEQQLIVDTTRAFVENELYPHEREVERSGHLPMELIKE
                     LQAKAIAAGLYAANMPEEVGGAGLDTLTWLLYEKELGRANYALHWTCVARPSNILLAG
                     TPEQREKYLFPCIRGEKWDCLAMTEPGAGSDLRGMKASAVQDGDDWVLNGTKHFISHA
                     DIADFAICFMASGEEETSGTSGRGKRKKITAFFVDKGTPGFTVRDGYRNVSHRGYTNA
                     ILEFDDCRLPKEQILGEVHKGFEVANSWLGATRLQVASTCLGRAERALGHSIEYAAQR
                     QQFGQQIGKFQGVSFKLADMAMELKAAELLVREAGWKYDKGTVTEEDMSMAKLKATEM
                     LAFVADEAIQIHGGMGLMDDLPLERIWRDARVERIWEGTSEIQRHIISRSLLRAVGG"
     gene            1680..>3578
                     /locus_tag="VGM46_10335"
     CDS             1680..>3578
                     /locus_tag="VGM46_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEY4193030.1"
                     /translation="MGEGNHRLDRLLRPKSIAIVGGGAFGTNVVEQCLKMGYAGEVWP
                     VHPSRADVHGVPAFKSVADLPGAPDAAFVAVNRNLTIDIIRHLAARGTGGAICFAAGF
                     LETGKDDADGAGLQKALVEAAGAMPIIGPNCYGLINYADGALLWPDQHGGKRLEKGER
                     GVAIITQSSNIACNLTMQTRGLPVAFLMTAGNQAQTGLSEMALGLIEDERVTALGLHI
                     EGFDSVAGFEKLAGRSRELKKPIVAMKVGRSEQARAATISHTASLAGSDAGSEAFLKR
                     LGIARVNSIPSFLETLKLLHAIGPLAGGRLSSMSCSGGEASVMADTAEGRGVAFPTLT
                     EAHRARVKESLGPLVAVANPLDYNTFIWNDEPAMAETFGAMVSGGFDLNMLVLDFPRT
                     DRCSDADWWTTVNAFEAALKANKAKGAIVTSMLENLPEGHAVDFLRRGIVPIHGIAEA
                     FDAAEAAAFIGAAWEKPSSPPIGGRGAERSEAERGTVDPQRPPPLRQGLPPSTPLPPM
                     GGDEKSQPNEAAAKAMLSQAGLPVPSGLEASSTDEAVAAAHELGFPVALKALGVAHKS
                     ELGAVALNLRDEAAVREAAHRLIGLGTGLYVERMVQGGIAELIVGFTRDPVFGPTMTL
                     GSGGVLVEL"
BASE COUNT          679 a         1146 c         1163 g          590 t
ORIGIN      
        1 catggcgcag ttcgggcctt gcctgaaatg gccatggacc aagctgatgg acgtgccgga
       61 gttcaacgac gagctggtcg acctgatcgc cacgcaatcg gacgagcagt cggacaaatg
      121 gtcgatccgc gagcttgaaa aaatccgtga cgacaatctg gtcgcgatca tggacgccct
      181 gtccaagcag aacaagggca agggctgggg cgccggcgcg ctgcataagg actatacgcg
      241 gcagctcgcc aaggccgcca agccggctgt gtcaaaagcc gcagccaagg caaaagccga
      301 caagccgaag aagaaagccg ccacaaagaa gaagggctga gccatggatt tcggcctttc
      361 cgaagaacag cagcttatcg tcgacactac ccgcgccttc gtggaaaacg agctttatcc
      421 gcatgaacgc gaggtggagc gcagcggcca cctgccgatg gaactcatca aggagttgca
      481 ggccaaggcg atcgccgccg ggctttacgc ggccaacatg ccggaagagg ttggcggcgc
      541 aggcctcgat acgctgacct ggctgctcta tgaaaaggaa cttggccgcg ccaattacgc
      601 gctgcactgg acctgcgtgg cgcggccttc caacattctg ctcgccggca cgccggaaca
      661 gcgcgaaaaa tatttgtttc cgtgcattcg cggcgagaag tgggattgcc tcgccatgac
      721 cgagccgggc gcgggttcgg acctgcgcgg catgaaggcc agcgccgtgc aggatggcga
      781 cgactgggtg ctcaacggca ccaagcattt catcagccat gccgacatcg ccgatttcgc
      841 catctgcttc atggcttcgg gcgaagagga aacctctggc acatccgggc gcggcaagcg
      901 caagaagatc accgcattct tcgtcgacaa gggtacgccc ggcttcaccg tgcgtgacgg
      961 ctaccgcaac gtctcgcatc gcggctacac caatgccatc ctcgaattcg atgattgccg
     1021 cctgcccaag gagcaaatac tgggcgaggt tcacaagggc ttcgaggttg ccaattcatg
     1081 gctgggcgcg acgcggcttc aggttgcctc gacctgcctt ggccgcgccg agcgcgcgct
     1141 tggccactcc atcgaatatg ccgcgcagcg ccagcaattc ggccagcaga tcggcaaatt
     1201 ccagggcgtg tcattcaagc ttgccgacat ggcgatggaa ctgaaggccg cagagcttct
     1261 ggtgcgcgag gccggctgga aatacgacaa gggcaccgtc accgaggaag acatgtcgat
     1321 ggccaagctg aaggccaccg aaatgctggc cttcgtcgcc gacgaggcga tccagattca
     1381 tggcggcatg ggcctgatgg atgatctgcc gctggagcgc atatggcgcg acgcccgcgt
     1441 cgagcgcatc tgggaaggca catcggaaat ccagcggcac atcatcagcc ggtcgctgct
     1501 gcgggccgtg ggcggttaat gcgcatcgcg gcaatcttta ccctccccct cgtggggagg
     1561 gtcgcccacg gggcggagtg gggatcatga cggaaaacaa cacatccatc cccacctcgg
     1621 cgctccgcgc cgacctgccg ggaagagcca ctggtctcgc ccgtcctgcg gaccccggca
     1681 tgggtgaggg caaccatcgc ctcgaccgtc tgctgcgtcc gaaatccatc gccatcgtcg
     1741 gcggcggcgc gttcggcacc aatgtcgtcg agcaatgcct gaagatgggc tatgccggcg
     1801 aagtgtggcc ggtgcatccg agccgcgcgg acgtgcatgg cgtgccggcg ttcaagtcgg
     1861 tggccgacct gcccggcgcg ccggacgccg ccttcgtcgc ggtgaaccgc aacctcacca
     1921 tcgacatcat ccgccatttg gctgcgcgcg gcaccggcgg cgcgatctgc tttgccgcgg
     1981 gtttcctcga aaccggcaag gacgacgccg atggcgcggg cctgcaaaag gcgttggtgg
     2041 aggcggcagg cgcgatgccg atcatcgggc cgaactgcta cggtctcatc aactatgccg
     2101 atggcgcgtt gttgtggccc gaccagcacg gcggcaagcg gctggaaaag ggcgaacgcg
     2161 gggtcgccat catcacgcaa tcgtccaaca tcgcctgcaa cctgaccatg cagacgcgcg
     2221 gcctgcccgt cgccttcctg atgacggccg gcaaccaggc gcagaccggc ctgtcggaaa
     2281 tggcgctggg actgatcgag gacgaacgcg tcaccgcgct tggcctgcac atcgaagggt
     2341 tcgattcggt ggccggcttc gaaaagcttg ccggccgctc gcgcgaactg aaaaagccga
     2401 tcgtcgccat gaaggtcggc cgctccgaac aggcgcgcgc cgccaccatt tcgcataccg
     2461 cttcgctcgc cggctccgat gccggatcgg aagccttcct gaaacggctt ggcattgcgc
     2521 gcgtcaactc cattccctct ttccttgaaa cgctgaagct gcttcacgcc atcggcccgc
     2581 ttgccggcgg gcggctgtct tcgatgagtt gttccggtgg cgaagcctcg gtaatggccg
     2641 acactgcgga aggccgaggc gtggccttcc ccactctcac cgaagcgcac cgtgcccgcg
     2701 taaaagaatc gctcggtccg ttggtggcgg tggccaaccc gctcgactac aacactttca
     2761 tctggaacga cgagccggcg atggccgaga cgttcggcgc gatggtgtcg ggcggcttcg
     2821 acctcaacat gctggtgctg gatttcccac gcaccgaccg ctgttcggac gccgactggt
     2881 ggacgacggt gaacgccttc gaagccgcgc tgaaggccaa caaggccaag ggcgccatcg
     2941 tcacttccat gctggaaaac ctgccggaag gccacgcggt ggactttttg cggcgcggca
     3001 tcgtgccgat ccatggcatt gccgaagcct tcgacgcggc ggaagctgcg gcgttcatcg
     3061 gcgcggcttg ggaaaaacct tcctcgcccc ccattggggg gagaggtgcc gagcgaagcg
     3121 aggcggagag ggggacggtt gacccccagc gccctccccc tctccgtcaa gggctaccgc
     3181 cctcgacacc tctccccccg atggggggcg acgaaaagag tcaacccaac gaagccgccg
     3241 ctaaggctat gctgagccag gcgggcttgc cggtgccgag cgggttggag gcttcctcca
     3301 ccgacgaggc cgtggccgca gcacacgagc ttggctttcc ggtagcgctc aaggcgctcg
     3361 gcgtggcgca taagagtgag cttggcgccg tcgcgctcaa ccttcgcgac gaggccgccg
     3421 tgcgtgaagc cgcgcaccgg ctgatcggcc ttggcaccgg gctttatgtc gagcgcatgg
     3481 tgcaaggcgg cattgccgag ctgatcgtcg gcttcacccg cgatccggtg ttcgggccga
     3541 cgatgacgct gggcagcggc ggcgtgctgg tcgaactg
//
LOCUS       DASYCS010000331         8271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3279543,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000331 DASYCS010000000
VERSION     DASYCS010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 8271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8271
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3279543"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1159
                     /locus_tag="VGM46_10340"
     CDS             <1..1159
                     /locus_tag="VGM46_10340"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY4193031.1"
                     /translation="VTVIDPLPPGGATSFGNAGMVRHDCNAPVAMPGMLRQVPGWLAD
                     PLGPLVLRPRYALKAAPWLLRWIRAGRMDRVNIAADALANLHRGNLDCYRDLIGSPAF
                     EALIRKVGGIQVWENETESLSDRITTEINARQGVVTEPLSRRDLERLLPGISSIARRG
                     LRYPENAHTINPHRLVTAIADAARQAGCVFATERVMKIIPRETGGYTLMTNTANRKAD
                     KVVIAAGAWSNRLLAPLRAALPLETERGYHLMLPSPSVELPVPVQMKSRGFALTPMEH
                     GLRLAGTVEIAGLEAAPDERRARILLTHARSLFPDLQGGEPTIWMGYRPSLPDSVPAI
                     GEAKGHAGLFIATGHGHTGMIGGGSTGRLVAELVTGHPPHIEPAPYSPGRF"
     gene            complement(1270..2559)
                     /locus_tag="VGM46_10345"
     CDS             complement(1270..2559)
                     /locus_tag="VGM46_10345"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY4193032.1"
                     /translation="MPYIDSYYRRTLSSDTDHPALSGRKNVDVCIVGGGLAGLTTALE
                     LLRRGRSVAVLEARRVAWGASGRNGGFVSPGWAAGADLIRRRVGADHAKALFSLSMEG
                     VSMVADAIRDLAVDDAGVTQGILRVARYDKSAALKAQRDEQQSEFGRTLRYLSRDEVR
                     SLLLSDKYHQALLDEASFHFHPLNYARALAREIVRLGGFVHEDTQVVSADLDSAEKRL
                     KTPDGEIVAATAVFAGGGYTDNVLPALRRAFLPIATYVVLSEAAPELIASAIRTTAGI
                     SDGRRAGDYYRVVDGGERLLWGGRITTRTTDPHDLAAVIRREIVTTYPQLGALKIDTA
                     WSGLMAYARHLMPLIGPVMPGVWSCTGFGGHGLNTTAIGGRVIAEAIAGDSDRYRLFA
                     PFGLVWNGGPFGTAAVQLSYWYLQGVDFVRERRAALA"
     gene            complement(2581..3264)
                     /locus_tag="VGM46_10350"
     CDS             complement(2581..3264)
                     /locus_tag="VGM46_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY4193033.1"
                     /translation="MQHYVDVTLSILPFLWSGFIQTIWISLVAIAAGSILGFVIGVVR
                     SQRVPVLHQLFGLYIHILRGSPFLVQLYVFYFVLPNTGIEMLQWSSEMAAFVSLSVYT
                     SSYVAEIIMGAIDTVPRGQWEAATSLGLKRIPILCLVVLPQALKLAVPPMSGVYVNII
                     KNTAILSVIGISELTRQGEISIMRFPRDVLFVYGLIALIYFIYCYPVLRFTRWAERKV
                     GGGRLINLD"
     gene            complement(3267..3962)
                     /locus_tag="VGM46_10355"
     CDS             complement(3267..3962)
                     /locus_tag="VGM46_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008597682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY4193034.1"
                     /translation="MNWVLDFYNYRIVEQYLGRFAEGLENTLIAAGVSLVAAVILGTI
                     LALGRMSGRAILWRPVAGYVAFVLATPLLVQIYLIYYGLPILVPATGRWSELTLGIMA
                     LSLHNAAYMSEIIRGGIGSVSRGQIEGGKAVGMSYLQRLRYIVLPQAFANTLPPLLGQ
                     TAVLIKDTSLLSIIAVFDFLSAGLLLNSERVRPNESFLTIAVGYLAIYAVMMSLSWAV
                     KRWLAGPAWNVGR"
     gene            complement(3998..4798)
                     /locus_tag="VGM46_10360"
     CDS             complement(3998..4798)
                     /locus_tag="VGM46_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008597681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY4193035.1"
                     /translation="MRNVLARAATSLAVMAAAWFTVATVAHADEMDDILKRGELIVAV
                     QTQGPPVSFVDKNGERTGFAVEFSREMAKDLGVKAVFQDYDWKGLIPALLAGKVDMIA
                     GDMTPTPQRASQLLFSRPMFFNDTIAFATKDSEFKSWEDLNKEGVTVAALQGSTYADA
                     VTKYLPKATLKVFAGGGPAVAQTLAMGRVNAMVGGQDYNTYVRQFDTLKPLDGVMTRE
                     PLAFATRPNAFHLKMWLDNYVELKTADNSLRDLLAYWWTSDAWEKDHK"
     gene            complement(4868..5548)
                     /locus_tag="VGM46_10365"
     CDS             complement(4868..5548)
                     /locus_tag="VGM46_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012067191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEY4193036.1"
                     /translation="MALAGVTTEIARGEVLVVFGPSGSGKSTFLRTLNALEAIDSGRI
                     VVDGVSLTDAGADLNKLRAEVGMVFQLFNLFQHRTALDNVMLPQIVVRRRSRAEALRR
                     SHELLAKVGIGDRAGHYPSQLSGGQQQRVAIARALAMDPKVMLFDEPTSALDPETVNG
                     ILDLMKILAAEGMTMAVVTHEMGFAREIADRMLFMESGLIVEDGSPQQIFHAPQQERT
                     RAFLDQIL"
     gene            complement(6093..6422)
                     /locus_tag="VGM46_10370"
     CDS             complement(6093..6422)
                     /locus_tag="VGM46_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013525116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HEY4193037.1"
                     /translation="MANRSVISFLTSVGTWTSHPSAFLVLLGYAVLWLIFDRSSLDWH
                     GAAALGTWAMTWFIQRAEHRDTQALHAKLDELLRAEGDADSSITQIDDAQPEDIEEHR
                     DRKQMGD"
     gene            complement(6554..6808)
                     /locus_tag="VGM46_10375"
     CDS             complement(6554..6808)
                     /locus_tag="VGM46_10375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HEY4193038.1"
                     /translation="MSELRKFTLVYAGLLLLLALTVSSSFIDLKGFNSALNLAIATAK
                     AVLIGLVFMQLSHEKTLPRLAVGATALWLIVLFGLTLAGQ"
     gene            complement(6805..7443)
                     /locus_tag="VGM46_10380"
     CDS             complement(6805..7443)
                     /locus_tag="VGM46_10380"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEY4193039.1"
                     /translation="MSTSTESIAFDTAAREKDADTFAMWVFVGSEAMLFGAIVLVFLV
                     ARLSYGDAFAPASRHLSLPLGTLNTAVLITSSFTMALAHMCANAARWRATIWSLTATA
                     LLGVVFLGIKAAEYTMEFHEGIAPVLGAPFIYDGPDPVHARFFFSLYFAMTSLHALHL
                     ICGIVVVAGMLAMWPRTVNSSRVRWVGSIGLYWHFVDIVWVFLFPILYLASR"
     gene            complement(7440..>8271)
                     /locus_tag="VGM46_10385"
     CDS             complement(7440..>8271)
                     /locus_tag="VGM46_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEY4193040.1"
                     /translation="CFSRRPLFGYKAVALSSMAIAVFGFLVWGHHMFVSGESAYAGVL
                     FSFLSFCVAIPSAIKVFNWSLTLRKGEITFETPMLYALFFLALFTFGGLAGLFLAALA
                     IDVHVHDTYFVVAHFHYIMVGGMVTAYMAGLHFWWPKMTGKMYSETMGRIAATVTFLG
                     FNLTFFPQFVLGFLGMPRRYHTYPPEFQFWHILSSAGAVVLAAGYLMPLFYLGWSLFR
                     GAKAPANPWGATGLEWQTASPPPPHNFETMPTVTRPAYDYEMKGLKHEKFSPHMDEAS
                     "
BASE COUNT         1715 a         2588 c         2527 g         1441 t
ORIGIN      
        1 cgtaacggtc atcgacccgc tgccgccggg cggcgccacc tcgttcggta acgcgggcat
       61 ggtcaggcat gattgcaacg cgcccgtcgc catgcctgga atgttgcggc aagtcccagg
      121 ctggcttgca gatccgctcg ggccgctggt tctgaggccg cgctatgcct tgaaggctgc
      181 gccttggctg cttcgctgga tacgggccgg ccgcatggac cgcgtcaaca tcgcggcaga
      241 tgcgctggcc aacctgcatc gcggcaatct cgactgttac cgcgacctga tcggctcacc
      301 ggcgttcgag gcgctgatcc gaaaggtggg cggtatccag gtctgggaaa atgaaacgga
      361 gagcctgtcc gaccgcatta ccacggaaat caatgcccgc cagggcgtcg tcaccgagcc
      421 gctttcccgg cgcgatctgg agcggctgtt gcccggcatt tcatcgatcg caaggcgcgg
      481 cctgcgctac cccgaaaacg cccacacgat caatccgcac cggctggtca ccgccattgc
      541 ggatgcggcg aggcaagccg gctgcgtttt cgcgaccgag cgggtaatga agataatccc
      601 gcgcgaaacc ggcggctaca cgctgatgac gaacaccgcc aaccgcaagg cggacaaggt
      661 ggtgatcgcc gccggcgcgt ggtccaaccg gctgcttgcg ccgcttcgtg cggccttgcc
      721 gctcgaaacc gagcgtggct atcacctgat gctgccgtcg ccctccgtcg agcttccggt
      781 gccggtgcag atgaaaagcc ggggctttgc gctgacgccg atggaacatg ggctgaggct
      841 tgccggaacg gtggagatcg ccggcctgga ggctgctccc gacgagcggc gcgccaggat
      901 cctgctcacc cacgcgcggt ccctgttccc ggacttgcag ggcggggagc cgacgatctg
      961 gatgggatac cgcccgtcct tgcccgacag cgtgccggcc ataggcgagg cgaagggcca
     1021 tgccggactg tttatcgcca ccggccacgg tcataccggc atgatcggcg gcggctcgac
     1081 cggcaggctg gtggccgaac tggtgacggg ccacccgccg cacatagagc ccgcgcccta
     1141 ttcgcccggc cgtttctgag ttttgctaga gcagttccag caaaagtgtg aaacggtttt
     1201 gcgcccggaa atgcgaccaa aacaaagaga tagagcggct ttgccgattc gataaaaacc
     1261 agaaccgctc tacgccagcg ccgcacggcg ttcgcgcacg aagtccacgc cctgaagata
     1321 ccagtacgac aattgcaccg cagccgtgcc gaacggcccg ccgttccaga ccaggccgaa
     1381 cggcgcaaaa agccggtagc ggtcgctgtc gccggcaatc gcttcggcga tgacccggcc
     1441 accgatggcc gtggtattca agccatggcc gccgaagccg gtgcaactcc agacgccagg
     1501 cataaccggg ccgatcaacg gcatcaggtg gcgggcatac gccatcaacc ccgaccacgc
     1561 cgtatcgatc ttcaacgcgc caagctgtgg ataggtggtg acgatctcgc gccggatgac
     1621 ggcggcgagg tcatgcggat cggtcgtgcg cgtggtgatg cgcccgcccc acagaagcct
     1681 ttcgccgcca tcgacgacgc ggtaataatc gccggcgcgg cggccgtcgc tgatcccggc
     1741 tgtcgtgcgt atcgcggatg cgatcagttc cggcgccgcc tcgctcagca cgacataggt
     1801 agcgatcggc aggaacgccc ggcgcaaggc cggcagcaca ttgtcggtat agccgccgcc
     1861 ggcaaacacc gcggttgcgg cgacaatttc gccgtccggc gttttcagcc ttttctccgc
     1921 actgtcgaga tcggccgaaa caacctgcgt atcctcatgg acgaaaccgc caaggcgaac
     1981 gatctcgcgc gccagcgccc ttgcataatt cagcggatgg aagtggaacg atgcttcatc
     2041 gagcagggcc tgatggtatt tgtccgaaag cagcaggctt cgcacctcgt cgcgcgacag
     2101 gtagcgcaaa gtgcggccaa attcgctttg ctgctcgtct cgctgggcct tgagcgccgc
     2161 gctcttgtca taacgggcga cacgcaggat gccttgggta acgccggcat cgtccaccgc
     2221 aagatcgcgg atcgcgtcgg ccaccatgga aacgccttcc atcgacagcg aaaacagcgc
     2281 cttggcgtgg tcggccccaa cgcggcggcg aatgagatcg gcccccgccg cccagccggg
     2341 cgagacgaag ccgccattgc ggccagacgc gccccaggcg acgcggcgcg cctcaagcac
     2401 ggcaacggag cggccccggc gcaatagttc gagcgctgtc gtcagcccgg caagcccgcc
     2461 gccgacaatg cagacatcga cattcttgcg gccggaaagt gccggatggt cggtgtccga
     2521 agacagcgtg cgccggtaat aactgtcgat ataggggatc attgcgggct cgttctcagg
     2581 ctagtcgagg ttgatcaatc ggccgccgcc gaccttgcgc tccgcccagc gcgtgaagcg
     2641 aaggaccgga tagcagtaga tgaaatagat cagcgcgatc agcccgtaga caaacaatac
     2701 gtcgcgcggg aagcgcatga tcgaaatctc gccctggcgc gtcagttccg aaatgccgat
     2761 caccgagagg atggcggtgt tcttgatgat gttgacatag acgccgctca tcggcggcac
     2821 cgcaagcttc agcgcctgcg gcaaaaccac gagacacagg atcggaattc gcttgagccc
     2881 aagcgaagtc gcagcctccc attgcccgcg cggcaccgta tcgatggcgc ccattatgat
     2941 ctcggcgaca tagctcgacg tgtagaccga cagcgacacg aaggccgcca tttcggaact
     3001 ccactgcaac atctcgatgc cggtgttcgg cagcacgaag tagaacacat agagctgcac
     3061 cagaaatggc gagccgcgca ggatgtggat gtagaggccg aaaagctggt gcagcaccgg
     3121 cacgcgctgg ctgcgcacga caccgatgac gaaaccgaga atggagccgg cggcaatggc
     3181 caccagcgat atccagatgg tctggatgaa accgctccag aggaacggca gtatggaaag
     3241 cgtgacatcg acatagtgtt gcatcgctac ctgcctacgt tccatgcggg gccggcaagc
     3301 cagcgcttca ccgcccacga caagctcatc atgacggcat agatggcgag atagcccacc
     3361 gcgatggtca ggaagctttc attgggccgc accctctcgc tgttgagcaa gagaccggcg
     3421 ctcaggaaat cgaacaccgc gatgatggac agaagcgagg tatccttgat cagcaccgcc
     3481 gtctggccga gaagcggcgg cagggtgttg gcgaaggcct gcggcagcac gatgtagcgc
     3541 agccgttgca ggtaggacat gccgacggcc ttgccgccct cgatctgccc gcgcgagacc
     3601 gagcctatgc cgccgcgtat gatctcgctc atataggcgg cgttgtgcag cgacagcgcc
     3661 atgatgccga gcgtcagttc gctccagcgg ccggtcgccg ggaccaggat cggcaatccg
     3721 tagtaaatca gatagatctg caccaatagc ggtgtcgcca gaacgaaggc gacgtagccc
     3781 gccaccggac gccagaggat cgcacggccg gacatgcggc cgagcgccag aatcgtgccg
     3841 agaatgacgg cggcgacgag actgacgccc gctgctatca gcgtgttttc gagaccctcg
     3901 gcgaagcgcc cgagatactg ctcgacgata cggtagttat aaaagtcgag aacccagttc
     3961 atccggtttc cgctttgtca ggccgaaccc gaacccgtta cttgtggtct ttctcccagg
     4021 catcggacgt ccaccagtaa gccagcaggt cgcgaagcga gttgtcggcg gtcttcagct
     4081 cgacatagtt gtcgagccac atcttcaggt ggaaggcgtt cggacgcgtg gcaaaggcca
     4141 gcggctcgcg cgtcatgacg ccatcgagcg gcttcagcgt atcgaactgg cgcacatagg
     4201 tgttgtagtc ctggccgccg accattgcgt tgacccgccc catggcaagc gtttgcgcca
     4261 ccgccggccc gccgccggca aacaccttca gcgtcgcctt gggcagatat ttggtgaccg
     4321 catcggcata ggtgctgcct tgcagggcgg cgaccgtcac gccttccttg ttgaggtcct
     4381 cccaggattt gaattccgaa tccttggtgg cgaaggcgat cgtgtcgttg aagaacatcg
     4441 gccgcgagaa cagaagctgg gaagcccgct gcggcgtcgg cgtcatatcg ccggcgatca
     4501 tgtcgacctt gccggccagg agcgccggaa tgaggccctt ccagtcatag tcctggaaca
     4561 cggccttgac gccgagatct ttcgccatct cgcggctgaa ctcgacggca aaaccggtgc
     4621 gctcgccgtt cttgtcgaca aaactcaccg gcggcccttg cgtctggacg gcgacgatca
     4681 gttcgccgcg cttgagaata tcatccattt catcggcatg ggcgaccgtt gcaaccgtga
     4741 accacgccgc cgccatcacg gccaggctcg ttgccgctct tgctaatacg tttctcattt
     4801 ttctctctcc ccttgttttg cagttgtctg atcgatcatg gttatggtgg ttatagaagt
     4861 ctgcggatca cagtatctgg tcgaggaacg cccttgtccg ctcctgctgc ggcgcgtgga
     4921 aaatctgctg cgggctgccg tcctcgacga tcaagccgct ttccatgaac agcatccggt
     4981 cggcgatttc gcgcgcgaac cccatctcgt gggtcacgac cgccatcgtc atgccctccg
     5041 cggccaggat tttcatcagg tcgagaatgc cgttgaccgt ttccgggtcg agcgccgagg
     5101 tcggctcgtc gaacagcatc accttcgggt ccatggccag cgcgcgcgca atggccacgc
     5161 gctgctgctg cccgccggac aactggctcg gataatgccc cgcccggtcg cctatgccga
     5221 ctttcgccag cagttcatgc gaccgccgca gcgcttccgc ccgcgaacgg cggcgcacca
     5281 ctatctgcgg cagcatgaca ttgtcgagcg ccgtgcggtg ctggaaaaga ttgaacagct
     5341 ggaacaccat gccgacttcg gcgcgcagct tgttgaggtc ggcgccggca tccgtgagcg
     5401 aaacgccatc gacgacgatg cgtcctgaat cgattgcctc cagcgcgttc aacgtacgca
     5461 aaaacgtgct tttaccggag ccggacggcc cgaacaccac cagcacctcg ccacgagcga
     5521 tttcagtcgt cacgccggca agagccagat gcgaacggcc atcatcggaa cgataggtct
     5581 tgcggatgtc ttcagcgacg atgatcggct tgcggtccgc atcatgcgca agggcgccac
     5641 cttcacgcgt gccgtttctt gtctcggtcg aactgtcgct gtgaccggcc aagcaatccc
     5701 ccaaattggc ttttttcttg gcgtcgcgta tccgcaggcg cccttcatga aagccgatgg
     5761 gattggccaa cacaagctat ttctttgccg gcggtcgaac caggaaaccg tggccccaaa
     5821 caggcgaagt cgcggccccg cgcggcatgc cctgtgcgga actggccaaa ccggaaccgg
     5881 ctgtctgccg aatgacgaac tgtcgatatc ttttccaggg acttcatccc cgcccactcg
     5941 cttcgcaggt gttgaacaat gatcgtttgt ctggcaatgg caacgccacc tttgcgacag
     6001 ccgtttcgcc cgcatgtcgc agcgtatggg ccatacgatg atttgagcgt cgcacatatt
     6061 ggctgcgcaa gcgcccagtc gaggatagag cctcaatctc ccatctgctt ccggtcccga
     6121 tgttcttcaa tatcctcggg ttgcgcatcg tcaatctggg tgatgctgct gtccgcgtcg
     6181 ccttcagctc gcagcaattc atccagtttg gcgtgaaggg cctgggtgtc gcgatgttcg
     6241 gctcgctgta tgaaccacgt cattgcccag gtccccaagg cggcggcgcc gtgccaatcg
     6301 aggcttgatc tatcgaatat cagccacagc acggcatagc cgagcagcac aaggaaagct
     6361 gacggatggg aagtccaggt gccaacggaa gtcagaaagc tgattaccga gcgatttgcc
     6421 atctcgcata accagcgagg ttggagcgag ttccggcggt ggcttcctat ttggcctttt
     6481 tgaagcgcgc gccttaccgc cggcggtagc ggcacgggag ccgtggcgcc gcgctcggca
     6541 gtcccacccg ccatcattgc ccagctaagg tcaggccaaa aagcacaatc agccacaggg
     6601 ccgtcgcccc tacagcaagg cgaggcagtg ttttctcgtg cgacagctgc atgaaaacca
     6661 ggccgatcag cacggccttg gcggtggcga tcgccaggtt gagcgcggaa ttgaagccct
     6721 tgagatcgat gaacgacgaa ctgaccgtca atgccagcag caacaacaag cccgcataaa
     6781 ccagagtgaa tttacgaagc tcgctcatcg gctggccaga tagagaattg gaaacaggaa
     6841 gacccagacg atatcgacaa aatgccagta caggccaatg gaaccgaccc agcgcacgcg
     6901 gcttgagttc acggttcgtg gccacattgc cagcataccg gcaaccacga cgataccgca
     6961 aatgagatgc aaggcgtgca gcgaagtcat cgcgaaatag aggctgaaga agaaccgggc
     7021 atgaacgggg tccggcccgt catagatgaa cggggcgcca agcaccggcg cgatgccttc
     7081 atggaattcc atcgtatatt ccgccgcctt gatgccgaga aagacgacac cgagcaaggc
     7141 ggtcgctgtc agcgaccata tggttgctcg ccaccgcgcg gcgttggcac acatatgcgc
     7201 cagcgccatg gtgaagctcg acgtgatcag cacagcggta ttgagcgtgc ccagcggtag
     7261 tgaaaggtgc ctggaagccg gcgcgaaagc gtcgccatag ctgagcctcg ctaccaggaa
     7321 caccagcact atagccccga acagcattgc ctctgaaccg acgaacaccc acatggcgaa
     7381 cgtgtccgca tccttttcgc gggctgccgt gtcgaaggcg atgctttcgg ttgaagtgct
     7441 catgaggctt cgtccatatg cggactgaat ttctcgtgct tcagcccctt catttcataa
     7501 tcataagcgg gccgggtcac tgtcggcatc gtttcgaaat tatgcggcgg cggcggcgac
     7561 gccgtctgcc attcaagccc ggtcgcaccc caagggttgg ccggcgcctt tgcgccgcga
     7621 aacagtgacc agccgagata aaacaacggc atcagatagc cggcggccag caccaccgcc
     7681 ccggcggaag agaggatgtg ccagaactga aattccggcg gataggtgtg atagcggcgc
     7741 ggcatgccga ggaagccgag cacgaattgc ggaaaaaagg tcaggttgaa accgagaaag
     7801 gtgacggtgg cggcgatgcg gcccatggtt tctgaataca ttttgccggt catcttcggc
     7861 caccagaaat gcaggccggc catataggcc gtcaccatgc cgccaaccat gatgtagtgg
     7921 aaatgggcca cgacgaaata ggtgtcatgg acatggacgt cgatggcgag cgccgcgagg
     7981 aaaagacctg ccagcccgcc gaaagtgaac agcgccagga aaaacagcgc gtaaagcatc
     8041 ggcgtctcga aggtgatctc gcccttgcgc agcgtcaggc tccagttgaa caccttgatc
     8101 gccgagggaa tggccacgca aaacgacagg aacgagaaca gcacgccggc ataggccgac
     8161 tctccggaga cgaacatgtg gtgcccccag accagaaagc cgaagaccgc gatcgccatc
     8221 gaggacaggg caacggcctt gtagccgaac agcggccggc ggctgaagca g
//
LOCUS       DASYCS010000332         9716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1103096,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000332 DASYCS010000000
VERSION     DASYCS010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 9716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9716
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1103096"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="VGM46_10390"
     CDS             complement(<1..559)
                     /locus_tag="VGM46_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193041.1"
                     /translation="MVALCAIAMTPLLAAGPALALSEIKREEIPAPTATPPAANEEAP
                     TDKSVPMPDPIGTTPPATPQPASPEDQQTPEQVDPKAPAQDGGIAHPRVEPNAPLPEV
                     IYDLGKLPEPVRRMHDLIVEACKSGDIEKLRPLVGQGDAMTQLSLTDIDGDAITFLKS
                     LSGDTEGQEILAIMEEVLNAGFVHLD"
     gene            complement(686..943)
                     /locus_tag="VGM46_10395"
     CDS             complement(686..943)
                     /locus_tag="VGM46_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193042.1"
                     /translation="MEHAVTDIDALVREEKRLTAVESHNEAWAEGLSAGIEPEIIAEA
                     ALETAFGEMLRANGEKAALALLDRMREKVIAGAFEPELARH"
     gene            complement(1235..1612)
                     /locus_tag="VGM46_10400"
     CDS             complement(1235..1612)
                     /locus_tag="VGM46_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02301 family protein"
                     /protein_id="HEY4193043.1"
                     /translation="MSRAALFIVALLAIGTAVSARPAHAAEAPFEPGLMRLAEILGSL
                     HFLRNLCGESGEQWRGEMEKLLQSENPDPERRARFIARFNRGYRSFGGTYTQCTASAR
                     EAIRHYMQEGGDLSRDIASRYGN"
     gene            complement(1676..2089)
                     /locus_tag="VGM46_10405"
     CDS             complement(1676..2089)
                     /locus_tag="VGM46_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010915126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY4193044.1"
                     /translation="MSEARKTLPAVSVAVVRGERVLLVKRARAPSLGLYAFPGGKVEA
                     GETLELAARRELREETGVEATEFRRLRDIFIEGGGEGHPVDYLLTVFGAAYAAGEAIA
                     ADDAETAEFYSLAEMEKLPLAGSVFTIAAELLRRR"
     gene            2215..2979
                     /locus_tag="VGM46_10410"
     CDS             2215..2979
                     /locus_tag="VGM46_10410"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEY4193045.1"
                     /translation="MCGRFALTATPDQTAAFLGLGELEDFPVRYNIAPTQPILMAMAA
                     ERRAPGSNLPDRQPILVRWGLIPTWVKDTRNFPLLFNARSEGAIEKASFRAAMRHRRA
                     LVPASGFYEWRQTENGRKGQPYWIRPRHGGLVAFAALMEAYAEPGGSEMDTGAIMTVN
                     ASQDIAHIHQRMPVMIEPEHFSQWLDCRGREPRDVAHLLRPADEGFFEAIPVADLVNK
                     VANTGPEIQERFTAADAPAPEKPRHKKTDDGQMTLF"
     gene            3013..3405
                     /locus_tag="VGM46_10415"
     CDS             3013..3405
                     /locus_tag="VGM46_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193046.1"
                     /translation="MQRRQFPHATRGAMNFWTGFFFLVVQSRAAAMAAGPIGRYCAAS
                     IGCLELRLSFLLPAIFLFPEISSSDFEYRNRFRPNRDFYLMLPGLFHDCADMFNKTLQ
                     TVFAGFLRSLFVETCDLTATRRTAIKQP"
     gene            3556..4992
                     /gene="cysS"
                     /locus_tag="VGM46_10420"
     CDS             3556..4992
                     /gene="cysS"
                     /locus_tag="VGM46_10420"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010915129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEY4193047.1"
                     /translation="MSEGSKGLRLYNTLTRTKEDFVPIDPDNVRIYVCGPTVYDFAHI
                     GNARPVIVFDVLFRLMRHLYGADHVTYVRNITDVDDKINARALRDFGPEISSGKLSLN
                     EAIRRVTEKTADQFHKDVAALGCLPPTFEPRATEFVLPREDGKSDMVTLIQTLVERGH
                     AYVAQGDVLFDTASMADYGQLSKRNLDEQQAGARIAVDMHKKNPQDFVLWKLSSAEEP
                     GWDSPWGRGRPGWHIECSAMSAAYLGEVFDIHGGGLDLIFPHHENEIAQSRCAHGTHA
                     MANIWMHNGFLQVEGQKMSKSLGNFYSINQLLETETFGGRKWPGEVLRLAMLMTHYRE
                     PIDFSVRKLEEAENTLRKWKRAADLATDSATALPPEIVAALSDDLAIYSAFQVLTQLA
                     ADATEGNDAAASLKAALEFLGFEVAATSVDNGAISTTIAERLAFIAAKNWAEADRIRD
                     ELLVQGIQLKDGKNPATGERTTSWEVKR"
     gene            4989..5372
                     /locus_tag="VGM46_10425"
     CDS             4989..5372
                     /locus_tag="VGM46_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY4193048.1"
                     /translation="MIDHLGITVSDFDASKAFYDQAMTPLGASLLMMVPLEYTDGVKV
                     GGYGRERPTFWINEGERRKDHQHVAFTARSRAEVDAFHAAAIAAGGKDNGGPGLRPQY
                     HPDYYAAFVFDPDGNNVEAVCHAPG"
     gene            5379..5870
                     /locus_tag="VGM46_10430"
     CDS             5379..5870
                     /locus_tag="VGM46_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010915131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY4193049.1"
                     /translation="MSLDNKPIYSGGCQCGAVRFRVEGALGDASVCHCRMCQKAGGGF
                     YLPLVSVNGAKLEWTRGAPKKFQSSNLARRGFCADCGTPLTYEAPDGVALAIAAFDHP
                     HDIVPTIQWGIEAKLPYVDAISGLPEQHTMADSDAPFLAELVSYQHPDHDTDQWPLPG
                     DQA"
     gene            5867..6829
                     /gene="pip"
                     /locus_tag="VGM46_10435"
     CDS             5867..6829
                     /gene="pip"
                     /locus_tag="VGM46_10435"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018427082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEY4193050.1"
                     /translation="MSELRTLYPEVEPFDSGMLDVGDGHMVYWERCGTKGAKPAVFLH
                     GGPGGAISPKHRRLFDPALYDVILFEQRGCGNSTPHASLEANTTWHLVADIERLREMA
                     GFDKWLVLGGSWGSTLALAYAEAHPQRVSELVVRGIYTLTRAELDWYYQFGVSQMFPD
                     KWERFVAPIPEEERGDMIAAYRKRLVGDDRAAQVEAARAWSTWEGETITLLPEPETSE
                     PFGQDDYALAFARIENHYFVHACWMDDGQLLRDAGRLKDIPGVIIHGRYDMPCPAHYA
                     WALHKAWPKADFFLIEGAGHAYSEPGILDRLIRATDGFAGKAAK"
     gene            6948..8564
                     /gene="cimA"
                     /locus_tag="VGM46_10440"
     CDS             6948..8564
                     /gene="cimA"
                     /locus_tag="VGM46_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HEY4193051.1"
                     /translation="MSRERLYLFDTTLRDGQQTPGIDFSVEDKIVIAKMLDEFGMDYV
                     EGGYPGANPTDTGFFERKRTTAAKFVAFGMTKRAGVSASNDPGLASLVQSKSDAICFV
                     AKSWDYHVRVALGCSNEENLEAIRESVEAARAAGKEALVDCEHFFDGFKANPAYALAC
                     AKTACEAGARWVVLCDTNGGTQPSEVRDIVRQVIAAGIPGENLGIHAHDDTGQAVANS
                     LAAVEGGVRQVQGTLNGIGERCGNANLVTIIPTLVLKPAFAGRFETGFSAQALTGLSR
                     LSRAFDELLNRAPAAQSPYVGQSAFATKAGIHASALAKEPATYEHVPPEAVGNRRRVM
                     VSDQGGRANFLAELARRGIEVPKSDSRLDALISMVKEREAEGYAYEGADASFELLARK
                     ILHGLPEFFEVSSFRCMIERRFDANGVLKTVSEAVVRIVVDGEEKMSVAEGHGPVNAL
                     DIALRKDLGKYQREIADLELADYKVRILNGGTEAITRVLIESFDATGARWWTVGVSDN
                     IIDASFQALMDSIVYKLMKNRDMAGLAAAE"
     gene            8639..9544
                     /gene="rarD"
                     /locus_tag="VGM46_10445"
     CDS             8639..9544
                     /gene="rarD"
                     /locus_tag="VGM46_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HEY4193052.1"
                     /translation="MSVEATTDSGKAAQSGFFYALLAYVLWGFLPFYMKAVAHIPAIE
                     VVAHRVIWSLPVAGALLLWLGRTDDIKRALRSPRTLMMGALTAALITVNWCVYVWAIS
                     VDRTLETALGYYINPLLSVLLGAVLLGEKLKPAQMVAIALAVLAVIILTWETGGLPWV
                     SIVLAVSWAFYELCKKTLPIGPAQGFFLEVLILAIPALAYVVWLQMTGAGHFGGQNGS
                     DTAWLMGAGLVTAVPLILYANGAKLLRLSTIGIMQYIAPTLIFMVAVFVFREPFSAET
                     AVAFGLIWVGLAIYTWSMVSHARQG"
BASE COUNT         1774 a         2980 c         3044 g         1918 t
ORIGIN      
        1 catcgagatg cacgaagccg gcattgagca cttcttccat gatggcgagg atttcctggc
       61 cttccgtatc gccggaaagg ctcttcagga aagttatcgc atcgccgtca atgtcggtca
      121 gcgacagttg cgtcatcgca tcgccctggc cgaccagcgg gcgcagcttc tcgatgtcgc
      181 cgcttttgca ggcctcgacg atcaggtcgt gcatgcgccg caccggctcg ggcagcttgc
      241 cgagatcgta gataacctcg ggcaggggcg cattcggctc gacgcgcgga tgcgcgatgc
      301 cgccatcttg cgcaggcgcc ttcgggtcga cctgttcggg cgtctgctgg tcttccggcg
      361 aggccggctg cggcgttgcc ggcggtgtcg tgccgatcgg gtccggcatc ggcacgcttt
      421 tgtcggtcgg cgcctcttcg ttggcggcgg gtggcgtcgc agtcggcgcg ggaatttcct
      481 cgcgcttgat ttcactcagc gccaaggcag ggccggccgc cagcagcggc gtcatcgcaa
      541 tggcgcataa agcgaccatt cccgtccaca acaccggcat gcgccgttca gccgacctgg
      601 aaaatttcac tgcccggttc tctccatggg cctctatgat gcccaaatgg tgtactgccg
      661 tcaaagagcc gccaagcggt ggccgttaat gccgtgccag ttccggttcg aatgcgccgg
      721 caatgacctt ttcacgcatg cggtcgagca gggcgagcgc cgctttctcg ccattggcgc
      781 gcagcatttc gccgaacgcg gtttcgagcg cggcttccgc gatgatttca ggctcgatgc
      841 cggccgaaag cccttcggcc caggcttcat tatggctttc gacagccgtc aggcgctttt
      901 cttccctgac cagagcgtcg atatcggtga cggcatgttc catattgcaa cttccacctt
      961 ttcaagcggc gattcgccca cctgatcaat tcctgctatc cgacgcggcg cccattaagc
     1021 cactgtagtt gcgttatttt tatgacctgc aacgaccagg acgtaacgtc gggtgaaagg
     1081 atggtagttc attcgacggc ttgccgacat gcaaaactaa gttttgagtt aattgcatcg
     1141 acgagcgtta gtgtttggtt agggtctgtg gccgttgcgc cacaatccgc ggctgaaatt
     1201 cgggtgccgg cctcgcctgg aaaccggcga agcctcagtt tccataccgc gaggcgatgt
     1261 cgcgcgacag gtcgccgcct tcctgcatgt agtggcggat cgcttcgcgc gccgaagccg
     1321 tacactgggt gtaggttccg ccaaacgagc gatagccgcg attgaagcgg gcgatgaacc
     1381 gggcgcgccg ttcagggtcg ggattttccg actgaagcag tttttccatc tcgccgcgcc
     1441 attgctcgcc gctctcgccg cacagattgc gcaggaaatg cagcgatccc agaatttccg
     1501 ccagccgcat caatccaggc tcgaagggcg cctccgccgc atgggcgggg cgtgccgaaa
     1561 ccgctgtgcc aatggccagc aatgcaacga taaagagggc ggcacggctc ataacccttt
     1621 gtgggcaagc aatttggcgt cggcaaggcg ctttgcgcgg ttgtgtgccg gtatttcagc
     1681 gacggcgcag caattctgcg gcaatggtaa agaccgagcc ggcaagcggc agcttttcca
     1741 tttccgccag gctgtagaat tcggccgtct cggcatcgtc ggcggcaatc gcctcgccgg
     1801 cggcataggc cgcgccgaac acggtgagca ggtagtcgac cggatggcct tcgccgccgc
     1861 cttcgatgaa aatgtcgcgc agccgccgga attccgtggc ctccacgcca gtttcttcgc
     1921 gcagttcgcg ccgggcggca agttccagcg tttcgcccgc ctccaccttg ccgccgggaa
     1981 aggcgtaaag gccgagcgac ggcgcccgcg cgcgcttgac caacagcacg cgctcgcccc
     2041 gaacgaccgc gacggaaacg gctggtagcg tctttcgtgc ctcgctcatc aaaatcctgc
     2101 ccgtcgcaac cagccagtcc gttgtggacg ggcggcgata gcagagcttt agcggttgcg
     2161 gcctcggcat tccaccgcca ctttcgccat aggccgaaac cggaagatgg acccatgtgc
     2221 ggacgatttg cgctgacggc gacgccggac cagacggccg cctttctcgg tctgggcgaa
     2281 ctggaagatt ttcccgtccg ttacaacatt gcgccgacgc agcctatcct gatggcgatg
     2341 gcggcggaaa gacgcgcgcc ggggtccaat ctgcccgacc gccagccgat attggtgcgc
     2401 tggggcctga tcccgacctg ggtgaaggat accaggaatt tcccgctgct gttcaatgcg
     2461 cgctccgaag gggccatcga gaaggcctcc ttccgcgccg ccatgcgcca tcgccgggcg
     2521 ctggtgccgg catccggctt ttacgaatgg cggcagacgg aaaacgggcg caagggccag
     2581 ccctattgga taaggccgag gcatggcggg cttgtcgcat tcgcggcgtt gatggaggcc
     2641 tatgcggagc ctggcggctc cgaaatggac accggcgcga tcatgaccgt gaatgccagc
     2701 caggatatcg cccatatcca ccagcgcatg ccggtgatga tcgagcctga gcatttttca
     2761 caatggctgg attgtcgggg cagggaaccg cgcgacgtgg cccacctgtt gcggccggcc
     2821 gacgagggct tttttgaggc aattccggtc gccgatctgg tcaacaaggt cgccaatacc
     2881 gggccggaaa tccaggagcg ctttacggcc gccgatgcac ccgcgcccga aaagccgagg
     2941 cataaaaaga ccgacgacgg ccagatgacg ctgttttagg cgcgcctgcc ggtttcatcg
     3001 gcgatgccca caatgcaacg gcgacagttt cctcacgcca cgcgcggcgc gatgaatttt
     3061 tggacgggct ttttcttcct ggtcgtgcaa agcagggcgg cagcaatggc cgccgggccg
     3121 attggccgat attgcgcggc aagcattggc tgcttggaac ttcggctttc tttcttgttg
     3181 ccggccattt ttctcttccc cgagataagt tcatctgatt tcgagtatcg taatcgattc
     3241 cggccaaacc gtgactttta cttgatgttg ccgggcctct ttcatgactg tgccgacatg
     3301 tttaataaaa ccttacagac agtgtttgcc ggcttccttc gctcgctttt cgtggaaaca
     3361 tgcgacttga ctgcaacacg ccgcacggcg ataaagcagc catgaaaacg tctttcgcca
     3421 tttgtggcat ttcgattatt ggctagcgct cgcgctggtc cggacgtttt cgcctcatct
     3481 tccttctaga ttggacaaac gccccggcca gcaggctgga ttttggcttg cgcctttgtg
     3541 cgtggaagga aacggatgtc tgagggatcg aagggcttgc gcctgtacaa tacgctgacg
     3601 cggacgaagg aggattttgt ccccatcgat cccgacaatg tgcgcatcta tgtctgcggc
     3661 ccgaccgtct acgacttcgc ccatatcggc aatgcgcggc ccgtcatcgt cttcgacgtg
     3721 ctgtttcgcc tgatgcgcca tctctatggc gccgatcacg tcacctatgt gcgcaacatc
     3781 accgacgtcg atgacaagat caacgcgcgc gccttgcgtg atttcgggcc ggagatttcc
     3841 tcgggcaagc tgtcgctgaa cgaggcgata agacgggtga cggaaaagac cgccgaccag
     3901 ttccacaagg atgtggcggc ccttggctgc ctgccgccga cgttcgagcc gcgcgccacc
     3961 gagttcgtgc tgccgcgcga agacggcaag tccgacatgg tgacgttgat tcaaaccctt
     4021 gtcgaacgcg gccatgccta tgtcgcgcaa ggcgacgtgc tgttcgatac ggcctcgatg
     4081 gccgactatg gccaattgtc gaagcgcaat ctggacgagc agcaggccgg cgcgcgcatc
     4141 gcggtcgata tgcataagaa aaacccgcag gacttcgtct tgtggaagct ttcgtcagcc
     4201 gaagaacccg gctgggacag cccatggggc aggggccgcc ccggctggca catcgaatgc
     4261 tcggccatgt cggccgccta tctcggcgag gtgttcgaca ttcacggcgg cgggctggac
     4321 ctgattttcc cgcaccatga aaacgagatc gcccagtcgc gctgcgccca cggcacccac
     4381 gccatggcca acatatggat gcacaacggg tttcttcagg tcgagggaca gaagatgtcc
     4441 aagagcctgg gcaatttcta ctccatcaac caattgctgg agaccgagac gttcggcggc
     4501 cgcaaatggc cgggcgaggt gctgcggctg gccatgctga tgacgcatta ccgcgagccg
     4561 atcgatttct cggtgcgcaa gctggaagag gcggagaata cgctgcgcaa atggaagcgc
     4621 gccgccgatt tggcaacgga cagcgcaacc gcgctgccgc cggaaatcgt ggctgcgctt
     4681 tcggacgacc tcgctatcta ttccgccttt caggttctga cgcaacttgc cgcggacgcc
     4741 accgagggaa acgatgccgc tgcatcgttg aaagcggcac tggagtttct tggctttgaa
     4801 gttgctgcaa cctcggttga caatggggca atctccacga ccattgccga acgcctggcc
     4861 ttcatcgcgg ccaagaactg ggccgaggcc gaccgcatcc gcgacgaact tctggtgcaa
     4921 ggcattcagt tgaaggacgg caagaacccc gcaacgggag aacgcactac aagctgggag
     4981 gtaaaaagat gatcgaccat ctaggcatta ccgtttccga tttcgatgcg tccaaagcct
     5041 tttacgatca agccatgacg ccgctcggcg cctcgctgct gatgatggtg ccgctcgaat
     5101 acaccgatgg cgtcaaggtc ggcggctatg gccgcgagcg cccgactttc tggatcaacg
     5161 agggcgagcg ccggaaggat caccagcacg tcgccttcac cgctcgcagc cgcgctgaag
     5221 tcgatgcatt ccatgcagcg gcaattgccg ccggcggcaa ggacaatggc gggccgggcc
     5281 tgcgcccgca atatcatcca gattattacg cagcctttgt tttcgatccc gatggcaaca
     5341 atgtcgaagc cgtctgccat gcgccgggtt gagcctctat gagcctcgac aacaaaccca
     5401 tctattcggg cggttgccag tgcggggcgg tgcgctttcg cgtcgagggc gcgctgggcg
     5461 acgcctccgt ctgccattgc cgcatgtgcc agaaggccgg cggcggcttc tatctgccgc
     5521 tggtgtcggt gaacggcgca aagctcgaat ggacgcgcgg cgcgccaaag aaattccagt
     5581 cttccaacct tgccaggcgt ggcttctgcg ccgattgcgg cacgccgctg acctatgaag
     5641 cgccagacgg cgtagcgctt gccatcgccg cctttgacca cccccacgac attgtcccta
     5701 ccatccagtg gggcatcgag gcgaagctgc cctatgtcga cgccatttcg ggcctgccgg
     5761 agcagcacac catggccgac agcgacgcgc cgtttcttgc ggaactggtt tcctatcagc
     5821 atcccgatca cgacaccgac cagtggccgc tgccaggcga ccaggcatga gcgaattgcg
     5881 caccctctat ccggaagtcg agccattcga cagcggcatg ctggatgtcg gcgatggcca
     5941 catggtctat tgggagcgtt gcggcacgaa gggcgccaag cctgccgtgt tcctgcatgg
     6001 cgggccgggc ggcgccattt cgccgaagca ccggcggctg ttcgacccgg cgctctacga
     6061 tgtcatcctg ttcgagcagc gcggctgcgg gaactcgacg ccgcatgcct cgctggaagc
     6121 caacacgaca tggcatctgg tcgccgatat cgagcggctg cgcgagatgg ccggcttcga
     6181 caaatggctg gtgcttggcg gttcatgggg ttcgacgctg gcgctggcct atgccgaagc
     6241 ccatccgcag cgtgtcagcg aactcgttgt gcgcggcatt tacacgctca cgcgggccga
     6301 actggattgg tactaccagt ttggcgtgtc gcagatgttt cccgataaat gggagcggtt
     6361 tgtcgcgccg atcccggagg aggaacgcgg tgacatgatc gccgcctatc gcaagcggct
     6421 ggtcggcgac gaccgggcag cgcaggtgga agcggcccgc gcctggagca cctgggaagg
     6481 tgaaacgatt acgctgctgc cggaaccgga aaccagcgag ccgttcggcc aggacgacta
     6541 cgccctcgct tttgcccgca tcgaaaatca ctatttcgtc cacgcctgct ggatggatga
     6601 cggccagctt ttgcgcgatg cgggccggct gaaggatatt ccgggcgtca tcatccatgg
     6661 ccgttacgac atgccgtgcc cggcgcacta tgcttgggca ttgcacaagg catggcccaa
     6721 ggccgatttt ttcctgatcg aaggggcggg tcacgcttac tccgagccgg gcatactcga
     6781 tcgattgatc cgcgccacgg atggtttcgc gggaaaggcg gccaaatgac cccggagcgc
     6841 ctttatattt tcgataccac cttgcgcggc ccgttcgacc gcagccgcgc agcgggcgag
     6901 gacggacaag gaatgaagat tggcgtcgcg cgatggagtg ctcgaccatg agccgtgagc
     6961 gcctctatct cttcgacacc accttgcgcg acggccagca gacgcccggc atcgattttt
     7021 cggtcgaaga caagatcgtc atcgccaaaa tgctggacga gttcggcatg gactatgtcg
     7081 agggcggcta tcccggcgcg aacccgaccg ataccgggtt tttcgaacgg aaacggacca
     7141 ccgccgcgaa gttcgtcgcc ttcggcatga ccaagcgggc gggggtgtcg gcgtcgaacg
     7201 atcccggcct tgcctcgctc gtgcaatcga agtcggacgc catctgcttc gtcgccaaaa
     7261 gctgggacta tcatgtgcgc gtagcacttg gttgcagcaa tgaggaaaac ctcgaagcta
     7321 tccgcgaatc cgtcgaggcc gcgcgcgcgg cgggcaagga ggcgctggtc gattgcgagc
     7381 atttcttcga cggtttcaag gccaaccccg cctatgcgct tgcctgcgcg aagacggcct
     7441 gcgaggccgg cgcgcgctgg gtggtgctgt gcgacaccaa tggcggcacg cagccatcgg
     7501 aagtgcgcga cattgtccgg caggtcatcg cggccggcat tccgggagag aatctcggca
     7561 tccatgctca tgacgatacc ggccaggcgg tggccaattc gctggcggcg gtggaaggtg
     7621 gcgtgcgcca ggtccagggc acgctgaacg gcatcggcga gcgctgcggc aacgccaacc
     7681 ttgtcaccat catcccgaca ttggtgctga aaccggcctt tgccgggcgt ttcgagacgg
     7741 gtttttcggc gcaggcgctg accgggcttt cgcgcctgtc gcgcgctttc gacgaattgc
     7801 tgaaccgcgc gccggcggca cagtcgcctt atgtcggcca gtcggctttc gccaccaagg
     7861 cgggcattca cgcttcggcg ctggccaagg aacccgccac ctatgagcat gtgccgccgg
     7921 aagcggtcgg caaccgccgc cgcgtcatgg tctcggacca gggcggccgg gcgaatttcc
     7981 tggccgagct cgcgcggcgc ggcatcgagg tgccgaagag cgacagccgc cttgatgcgt
     8041 tgatttccat ggtcaaggag cgcgaggccg agggttatgc ctatgaaggc gcggatgcga
     8101 gcttcgagct tctggcgcgc aagattctgc atggcctgcc ggaatttttc gaagtcagca
     8161 gttttcgctg catgatcgag cggcgcttcg atgccaatgg cgtgctgaag accgtatcgg
     8221 aagcggtcgt caggatcgtc gtcgatggcg aggaaaaaat gtcggtggcc gagggccacg
     8281 gccccgtcaa cgcgctggac atcgcgctgc gcaaggacct tggcaaatat cagcgcgaaa
     8341 tcgccgacct cgaactggcc gactacaagg ttcgcatcct caatggcggc accgaggcga
     8401 tcacccgcgt cctgatcgaa tccttcgacg ccaccggcgc gcgctggtgg acggtcggcg
     8461 tgtcggacaa catcatcgac gcctcgttcc aggcattgat ggattccatc gtctacaagc
     8521 tgatgaagaa ccgcgacatg gccgggctgg ccgccgccga ataaccatca gcaaaagtcc
     8581 attgacccat cagttatttg cgatggtgcg ctgagcgaat taccgccata ccggcacgat
     8641 gagcgtcgaa gcgacaacag attccggcaa ggccgcgcaa agcggcttct tttacgccct
     8701 tttagcctat gtgctgtggg gcttcctgcc gttttacatg aaggcggtcg cgcatattcc
     8761 ggcaatcgag gtggtggcgc atcgcgtgat ctggtcgctg ccggtcgccg gcgcgctgtt
     8821 gctgtggctt ggccgcaccg acgatataaa gcgcgccctg cgcagcccac gcacgctgat
     8881 gatgggcgcg ctgactgcgg cgctgataac cgtcaactgg tgcgtctatg tgtgggcgat
     8941 ctcggtcgac cgcacgctgg agacggcgct gggctattac atcaacccgc tgctctcggt
     9001 gttgttgggc gcggtgctgc tcggcgaaaa gctgaagccc gcgcagatgg tcgccatcgc
     9061 gctcgcggtg ctggccgtca tcatcctgac atgggaaacc ggcggcctgc catgggtgtc
     9121 gatcgtgctt gccgtttcgt gggcgttcta cgagctgtgc aagaaaaccc tgccgatcgg
     9181 tccggcgcag ggtttcttcc tcgaagtgct tatcctggct attcccgctt tggcctatgt
     9241 ggtctggctg caaatgaccg gcgccggcca tttcggcggt caaaacgggt ccgatacggc
     9301 atggctgatg ggcgccggcc tcgttactgc ggtcccgttg atcctctatg ccaacggggc
     9361 gaagctgctg cggctatcca ccatcggcat catgcaatat atcgcgccga cgctgatctt
     9421 catggttgcg gtattcgtct tccgcgaacc gttttcggcg gagacggcgg tggcgttcgg
     9481 ccttatctgg gtgggattgg cgatctatac atggtcgatg gtttcgcacg cgcgccaagg
     9541 ctgagacgac agcgctgaaa cgctaaagct tgtcgagcac cgcctcgtcg ccatcggctg
     9601 aaatgccgtt ggccgaagcc gccgtcaaaa tggcggcgac gatggcctcg gggtcgtcga
     9661 ccaccagcgg ctgaacgcgt cgcgcggtat ggatgaagcc ctcggtcgtc atatgc
//
LOCUS       DASYCS010000333         5333 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_525729,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000333 DASYCS010000000
VERSION     DASYCS010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 5333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5333
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525729"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /gene="tsf"
                     /locus_tag="VGM46_10450"
     CDS             complement(<1..809)
                     /gene="tsf"
                     /locus_tag="VGM46_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015317779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEY4193053.1"
                     /translation="MSISAAQVKELRDLTGAGMMDCKTALAETNGNMEEAVDWLRKKG
                     ISKADKKAGRTAAEGLIGVDAGLREASIVEVNSETDFVARNGAFQEIVANVAKVSLAY
                     GSTEAVAAAKYPGSDKSVTDTIKDAVGTIGENLGFRRSAKLTVPHGSVATYVHNAVAD
                     NLGKLGVLVAIETTGDAHKANAFARQVAMHIAAINPLALNTDELDPATVEREKAIFSD
                     QARASGKPENIIEKMVEGRLRKFYEEVVLLKQAFVLNPDVTVEQALKEAEK"
     gene            complement(959..1750)
                     /gene="rpsB"
                     /locus_tag="VGM46_10455"
     CDS             complement(959..1750)
                     /gene="rpsB"
                     /locus_tag="VGM46_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEY4193054.1"
                     /translation="MALPDFSMRQLLEAGIHFGHQTHRWNPKMGPYIYGARNNIHIID
                     LSQTVPLLHQALKQVSDTVARGGRVLFVGTKRQASDIVADAAQRSAQYYVNSRWLGGM
                     LTNWKTISNSIQRLRKLDELLAGEAQGLTKKERLNLDREREKLDKALGGIKDMGSTPD
                     LMFVIDTNKEAIAILEAKRLGIPVVAIIDSNCDPDKIDFPIPGNDDAARAIQLYCDLI
                     AKAAIDGIARQQGALGVDVGASIEAPVEPALDPAPEASSDEAPQA"
     gene            complement(1922..2626)
                     /locus_tag="VGM46_10460"
     CDS             complement(1922..2626)
                     /locus_tag="VGM46_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012092789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY4193055.1"
                     /translation="MSAQKVAIITAGGSGMGAGAAKRLAADGFKVAVLSSSGKGEALA
                     KELGGIGVTGSNQSADDLQRLVDLTVKEWGRIDVLVNSAGHGPRAQLLEISDDDWHKG
                     MDTYFMNVVRPVRLVTPVMQKQKSGSIINISTAWTFEPSSMFPTSAVFRAGLATYTKL
                     FADTYAADNIRMNNVLPGWIDSLPGTEERRESVPMQRYGTTEEIAATIAFLASEGAGY
                     ITGQNIRVDGGLTRSV"
     gene            complement(2755..3033)
                     /locus_tag="VGM46_10465"
     CDS             complement(2755..3033)
                     /locus_tag="VGM46_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4031 domain-containing protein"
                     /protein_id="HEY4193056.1"
                     /translation="MAVYVDAAIWKWAGKLWCHMLADDTDELHRFAQRLAIKRSSYQG
                     PPKTSAPHYDITGLERDRAVRLGAVECSREEIVMVFRRVKVANGKAPR"
     gene            complement(3058..3894)
                     /locus_tag="VGM46_10470"
     CDS             complement(3058..3894)
                     /locus_tag="VGM46_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity EipB family protein"
                     /protein_id="HEY4193057.1"
                     /translation="MRAKRLAFYALLVSAVSVAAPAFAVPMLQAHRAVYDLSLDKASD
                     RSGITGITGRMVYEFNGSPCEGYTVNFRFVTQIATSDSTRLSDQQTTTYEDSKGKTFS
                     FVTKSFVDQNLDKEVKGTAHKKDKGVEVDIDKPEKNTVHLSATQFPTQHLVELIDKAE
                     KGETFYETNLFDASEDADKVMTTTVVVGKKTMADKSDPELPALAKLGKDKYWPVDIAY
                     FDLSKKDSGEEVPAYRISFKLHENGLTRDLVMDYGDFSMTGKLVNLSLFDNAPKKACS
                     NK"
     gene            4034..4498
                     /locus_tag="VGM46_10475"
     CDS             4034..4498
                     /locus_tag="VGM46_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008875919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY4193058.1"
                     /translation="MSETIEKRLSDLGVTLPVAAAPAANYVPYVRTGDLLFPSGQLPM
                     KDGKLAFTGLLGRDLDTAAGKEAAKYCAINVLAQAKAALGDLEKIRRLVRISVYVASM
                     PDFTEQHLVGNGASDLFAQVLGERGKHARIAIGMASLPLNAAVEVESIFEVE"
     gene            4512..5240
                     /locus_tag="VGM46_10480"
     CDS             4512..5240
                     /locus_tag="VGM46_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006205156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEY4193059.1"
                     /translation="MTDLSWLIARPVAHRGFHDMNKTRWENTLSAFSAAVERNYAIEC
                     DAHLSSDGVPVIIHDDNLKRLAGEDGFVWQRTAAELAAMRVGGTGDHIPTLEEVLKLV
                     DGRVPIVVEVKGTPGRDSALVERVGKQLTRYKGKVAIMSFDHWLIRDFSRYAPGIPGG
                     LTAYGRDNNLIEAHFSMLAHDLAFTSYAAGDLPNPFVSFVREKLKMPVITWTVHDQPA
                     VDLTFKYADQMTFEGFEPDAVRIA"
BASE COUNT          961 a         1634 c         1680 g         1058 t
ORIGIN      
        1 tccttctccg cttccttcag cgcctgctcg acggtgacgt cgggattgag cacgaaggcc
       61 tgcttcaaca gcacgacttc ctcgtagaac ttgcgcagac ggccttcgac catcttctcg
      121 atgatgtttt ccggcttgcc ggaagcgcgc gcctggtcgg agaagatcgc cttctcgcgc
      181 tcgacggttg ccgggtccag ttcgtcggtg ttcagcgcca gcgggttgat cgccgcgatg
      241 tgcatggcaa cctggcgggc gaaggcattg gccttgtgcg catcgccggt cgtctcgatg
      301 gcgaccagca cgccgagctt gccgagattg tcggcaaccg cgttgtggac ataggtggcg
      361 acggagccat gcgggaccgt gagcttggcg gagcggcgga agccgaggtt ttcgccaatg
      421 gtgccgacgg cgtccttgat ggtgtcggtg accgatttgt cggaacccgg atatttcgct
      481 gctgcgaccg cttcggtgct gccgtaggcc agcgacacct tggcgacatt ggcgacgatt
      541 tcctggaagg cgccgttacg ggcaacgaag tccgtctcgg aattgacctc gacgatcgag
      601 gcttcgcgca ggccggcatc gaccccgatc aggccctcgg ccgccgtgcg gccagctttc
      661 ttgtcggcct tggagatgcc cttcttgcgc agccagtcga cagcttcttc catattgccg
      721 ttggtctcgg ccaaagccgt cttgcagtcc atcatgcctg cgccggtcag gtcgcgcagt
      781 tctttgacct gtgctgctga aatgctcatt tatcgcctct ttggttctgg aacgcattcg
      841 tcgtgaacgc gtggcgtcct gaataaacgg gcgcaccatc gagggatcga tggtgcgcaa
      901 attgcggcgc gtggccgcaa cgataacgag gaatgccgct gggaagcggc cttcctgttt
      961 acgcctgcgg ggcttcgtcg gacgaagctt ccggcgccgg atcgagggcc ggctcgacgg
     1021 gggcttcgat cgaagcgccg acatcgacgc cgagcgcgcc ctgctggcgg gcgatgccat
     1081 cgatggcggc cttggcgatc aggtcgcaat aaagctggat ggcgcgggcc gcgtcgtcat
     1141 tgccggggat cgggaagtcg atcttgtccg gatcgcagtt cgaatcgatg atggccacga
     1201 ccgggatgcc gaggcgcttg gcctcaagaa tggcgatcgc ctccttattg gtgtcgatca
     1261 cgaacatcag gtccggggtc gaacccatgt ccttgatgcc gcccagcgcc ttgtcgagct
     1321 tctcgcgctc gcggtcgagg ttcaggcgct ccttcttggt gaggccctgg gcctcgccgg
     1381 ccagcagctc gtccagcttg cgcaggcgct ggatggaatt ggagatcgtc ttccagttgg
     1441 tcagcatgcc gccgagccaa cgcgaattga cgtaatactg ggccgaacgc tgcgcggcat
     1501 cggcaacgat gtcggacgcc tggcgcttgg tgccgacgaa caggacgcgg ccgccacggg
     1561 caacggtgtc ggaaacctgc ttaagggcct ggtgcagcag cggaaccgtc tgcgacaggt
     1621 cgatgatgtg gatgttgttg cgggcgccgt aaatatacgg acccatcttc gggttccagc
     1681 ggtgggtctg gtggccgaag tgaatgccag cttccaaaag ctggcgcatg ctgaaatcag
     1741 gcagagccat tcgaagttcc tttccggtta gcctccacgg acacaggcga gggaaggatt
     1801 tccttgtcgc caccggaggt ttcagccgga tttctcccgg cagacaccaa agtccgtgtg
     1861 tggaatgagc gggcctataa aggctaaacg ccgcaaacgc aagcgctgtg ggacgttacc
     1921 gtcaaaccga gcgcgtcagg ccgccatcga cgcggatatt ctggccggtg atgtagcccg
     1981 cgccttccga agccaggaag gcgatggtcg ccgcgatctc ctcggtggtg ccgtagcgct
     2041 gcatcggcac cgattcgcgg cgctcctcgg tgcccggcag gctgtcgatc cagcccggca
     2101 gcacgttgtt catgcggata ttgtcggcgg cgtaggtgtc ggcgaacagc ttggtgtagg
     2161 tggcaaggcc cgcccgaaaa accgccgatg tcgggaacat ggacgacggc tcgaacgtcc
     2221 aggccgtcga gatattgatg atcgagccgg atttctgctt ctgcatgacc ggcgttacca
     2281 ggcggaccgg gcgcaccacg ttcatgaaat aggtgtccat gcccttgtgc cagtcgtcgt
     2341 cgctgatttc gagcagctgc gcgcgcgggc cgtggccggc gctgttgacc agcacgtcga
     2401 tgcggcccca ttccttcacc gtcaggtcga ccagtcgctg aaggtcgtcg gcggactggt
     2461 tcgagccggt aacgccgata ccgcccaatt ccttggcaag cgcctcgccc ttgccggacg
     2521 aagacagcac cgccaccttg aagccatcgg ctgcaagccg tttcgccgcg ccagcaccca
     2581 tgccggaccc gccggcggtg atgatcgcta ccttttgtgc cgacatgatc gttcctccct
     2641 attttaccga tgcgccttca cggcgcttgg cgtcagcgat aacgcttcgg ctccatgacg
     2701 cgaaggggca tggcgatcat tatatggtgc caaaggcctg ccgccgcccg ctaactagcg
     2761 cggcgccttg ccgttcgcca ccttcacccg ccggaaaacc atgacgatct cctcgcggga
     2821 acactccacc gcgccgagcc ggacggcgcg gtcgcgttcc agcccggtaa tgtcataatg
     2881 cggcgcggag gttttaggcg gcccctgata ggacgagcgc tttatcgcca gcctctgcgc
     2941 gaaccggtgc aattcatccg tatcgtcggc cagcatgtgg caccacagct tgccggccca
     3001 tttccagatc gccgcatcga cataaaccgc catcagaccg ccacggctcg ggcttgccta
     3061 tttgttgctg caagctttct tcggcgcatt gtcgaacagc gacaggttga ccaacttgcc
     3121 ggtcatcgag aaatcgccgt aatccatcac cagatcgcgc gtcagcccgt tttcatgcag
     3181 cttgaaactg atgcgatagg ccggaacctc ctcgccgctg tcctttttgg aaaggtcaaa
     3241 ataggcgata tcgaccggcc agtatttgtc cttgccgagc ttggccagcg caggcagctc
     3301 gggatcgctc ttgtcggcca tggttttctt gccgacgaca acggtagtgg tcatcacctt
     3361 gtcggcatct tccgaggcgt cgaacaggtt tgtttcgtag aaggtttcgc ccttctcggc
     3421 cttgtcgatt agctcgacca ggtgctgggt cggaaactgg gttgcggaaa ggtgcacggt
     3481 gttcttttcc ggcttgtcga tatcgacctc gacgcccttg tccttcttgt gcgccgtgcc
     3541 cttcacctcc ttgtcgagat tctggtcgac aaaggacttg gtgacgaagg agaacgtctt
     3601 gcccttggaa tcctcgtacg tcgtcgtctg ctggtcgctg aggcgggtgc tgtcgctggt
     3661 tgcgatctgc gttacgaagc ggaaattgac cgtatagccc tcacagggcg agccgttgaa
     3721 ctcatagacc atgcggccgg tgatgccggt aatgccggaa cggtcggatg ccttgtcgag
     3781 cgacaggtcg tagaccgcgc gatgcgcttg cagcatcgga accgcgaagg ccggggccgc
     3841 caccgaaacg gccgacacca gtaaagcgta gaatgcaagg cgctttgcgc gcatggacag
     3901 gctccggcaa ggcgcggctg gtggcagccg ccagggaaag atggtccagg tagaaaaaag
     3961 tcgtggcgga agctgggcaa aaatagcact ttcgcccagc cgacgcggca ccagcaagca
     4021 atagggaaat aagatgagcg aaacaatcga aaagcggctg agcgatctgg gcgtcacgct
     4081 tcccgtcgcg gccgcacccg ccgccaacta tgtgccctac gtcaggaccg gcgacctgtt
     4141 gtttccttcg gggcagttgc cgatgaagga cggcaagctc gccttcaccg ggctgctcgg
     4201 ccgcgatctg gacacggcgg ccggcaagga agccgccaaa tattgcgcca tcaacgttct
     4261 ggcgcaggcc aaggccgccc ttggcgacct cgaaaagatc cgccggctgg tgcgcatcag
     4321 cgtctatgtc gcgtcgatgc cggacttcac cgagcagcat ctggtcggca acggcgcttc
     4381 cgacctgttt gcgcaagtgc tgggcgaacg cggcaagcat gcccgcatcg ccatcggcat
     4441 ggcttcgctg ccgctcaatg cggcggtcga ggtcgagtcc atcttcgaag tagagtgagc
     4501 gagcgcggca catgaccgat ctttcctggc tgattgcccg gcccgtcgcc catcgcggct
     4561 tccacgacat gaacaagacg cgctgggaaa acacgctgtc ggccttcagc gccgccgtcg
     4621 agcgcaacta cgccatcgaa tgcgatgcgc acctgtcgtc cgatggcgtg cccgtcatca
     4681 tccatgacga caatctcaag cggctggccg gcgaggacgg ttttgtctgg cagcgcaccg
     4741 cagccgagct tgccgccatg cgcgtcggcg gcacgggcga ccatataccg acgctggaag
     4801 aggtgctgaa gctcgtcgac ggccgcgtgc cgatcgtcgt cgaggtcaag ggaacaccgg
     4861 ggcgcgacag cgcgctggtc gaacgggtcg gcaagcagct tacccgctac aagggcaagg
     4921 tggcgatcat gtcgttcgac cactggctga tccgcgactt ttccagatac gcgcccggca
     4981 ttccgggcgg gctgaccgcc tatggccgcg acaacaacct gatcgaggcg catttttcga
     5041 tgttggcgca cgatctcgcc ttcacctcct atgcggcagg cgacttgccg aacccattcg
     5101 tcagcttcgt gcgcgaaaag ctgaaaatgc cggtcatcac atggacggtg cacgaccagc
     5161 ccgcggttga cctgaccttc aaatatgccg accagatgac gttcgagggt ttcgagccgg
     5221 atgccgtgcg tattgcctga tgttcctcga catcgggctt gcatggacgt ggcgccaact
     5281 taagtaagtc ccatggatca gggcaacgaa gcaggcgaag taccaccggc gca
//
LOCUS       DASYCS010000334         3407 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4297157,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000334 DASYCS010000000
VERSION     DASYCS010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4297157"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..96
                     /locus_tag="VGM46_10485"
     tRNA            20..96
                     /locus_tag="VGM46_10485"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:54..56,aa:Arg,seq:acg)
     gene            complement(680..1663)
                     /locus_tag="VGM46_10490"
     CDS             complement(680..1663)
                     /locus_tag="VGM46_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007822817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HEY4193060.1"
                     /translation="MKKLPIDTYMLLLLATVALATILPVSGTSAKVVSQLSSAAVALL
                     FFLYGAKLKTQAIVAGIANWKLQLMVVLTTFGLFPLLGLGLAFLARPWLPEELVVGFL
                     FLSILPSTVQSSIALTAMARGNVPAAICAASLSNMLGVVVTPAFAALLLSTNGIKLDA
                     NAVIGICVQLLLPFVAGQLMRPLIGGWIEKNKLLTMIVDRGSILLIIYSAFSAGVVAG
                     IWGRLDGMTLSLLIVLDLALLLLVVTIVRRTGRLIGLSREDEVSVLFCGSQKSLASGV
                     PMANILFAGHGTGLIILPLMLYHQFQLFLCAAIAQRYVANDARDAVAQAKA"
     gene            1878..2759
                     /locus_tag="VGM46_10495"
     CDS             1878..2759
                     /locus_tag="VGM46_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012452612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY4193061.1"
                     /translation="MAPHKLLDVHPSLLRSFLAVANSLSFTTAGARLDLRQSTVSQHV
                     RRLEEAVGQRLLLRDTHSVALTEQGDAMVEMARTVLDANDRLANFFIDNGKRERLRLG
                     ISEDFAMSRLANVLIQFRRNDPHVDIELTVGLSGYLYQRFDAGELDVIFVKRKPGDKR
                     GKTAWRERPAWIARPGLRVPPGSPVPLVAFSPPSITRSLATDTLDRAGRPWRITCSSG
                     SISGLRAALLAGLGVAAHSSRLIPMGLMRLPEDAGLPQLPEIEFVAIGPGRTQRLAMR
                     MVDTLVDISESLQQVWE"
     gene            complement(2784..3236)
                     /locus_tag="VGM46_10500"
     CDS             complement(2784..3236)
                     /locus_tag="VGM46_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193062.1"
                     /translation="MENTLSSALSVTRIIVTWESRWPLNIYRDADEAKLSAGMRQRLA
                     DAEQMTIDDYRRGILERERGRAIYAALLDEGVDACITLSANGPAPIGLASTGDPGFAT
                     PCAYLGVPALSLPLLTFEGLPVGIQLMGFRDRDADLCAIAASIQQHLG"
BASE COUNT          645 a          983 c         1129 g          650 t
ORIGIN      
        1 cgtctagcaa gcctgttgcg cgcccgtagc tcagctggat agagcaccag actacgaatc
       61 tgggggtcag gagttcgaat ctcttcgggc gcgccatttt atcatcataa aatcggattt
      121 cggccgtttc atcgcggatg acagcgggta gtttcgtgca tggaactgtc acgggcatgg
      181 ttatggcagc gtggatttga taggcggtcg tcttgtctgg cggtctgggc aatatgtcga
      241 cggatatccg tccggatgag cctggttaat ccgagggcgg agcgtgtcgt tgcctgtggt
      301 ttgatcggtc tcgtctatct ccacacacag cgccgattaa gaacgcagac ggtctgcggg
      361 aggtggagaa ggacgtcgcg catcgagcat ggcgaagccg ggttggatcg acgtgccgca
      421 ggcggcctgg gtccgcttat ggacttgggc aggttcgcgg cctcatgccc gttgcttttt
      481 tttggcgaac ttcgggacgc gtgcggtgcg gctggctgcg cgattttcgg caatgtcggc
      541 catactcggc ggtgaggtgc gaatagctcg gcggtgaggt gccaatgatt ttggtgttga
      601 tatgccggcg gcgagctaaa cgcggcgggg tcggcgggtt caggggcgct cgctcctgaa
      661 cccggacggc gtgggggcgt caggccttgg cttgagccac ggcgtcgcgg gcgtcgtttg
      721 ccacgtatcg ttgagcaatg gcggcgcata ggaagagctg gaactgatga taaagcatca
      781 gcggcaggat gatcaggccg gtgccgtggc cggcaaaaag gatgttggcc atcggaacgc
      841 cgctggcgag gctcttttgc gaaccgcaga agagaacgct gacttcatcc tcgcgcgaca
      901 gtccgatcaa ccggccggtc cgcctgacga tggtcaccac cagaagcagc agcgccaggt
      961 cgagtacgat cagcagcgac agtgtcattc cgtcgagcct accccatatt cctgccacga
     1021 cgccggcgct gaatgccgaa tagatgatga gcaggatcga cccgcgatcg acgatcatcg
     1081 tcaaaagctt gttcttttca atccagccgc caatcagcgg ccgcatcaac tggccggcca
     1141 cgaagggcag caacaattgc acgcagatgc cgatcaccgc gttggcgtcg agcttaatgc
     1201 cgttggtcga tagcagcagc gcggcgaagg ccggcgttac caccacgccc agcatgttgg
     1261 agagcgaggc ggcgcaaatg gccgccggca cgttgcccct ggccatggcg gtcagcgcga
     1321 tggacgattg cacggtagac ggcaggatcg aaaggaagag gaagccgacg acgagttctt
     1381 ccggcaacca gggcctggcg agaaaagcga ggcccaggcc aagcagcgga aacaggccga
     1441 acgtggtgag caccaccatc aattgcagtt tccagttggc gatgccggcg acgatggcct
     1501 gcgtcttgag ctttgcgccg tacaggaaaa acaaaagcgc gaccgctgcc gagctaagct
     1561 gcgaaacgac tttcgccgaa gtgccggaca ccggcaggat cgttgccagc gcgacggtcg
     1621 cgagcagcaa cagcatatag gtatcgatgg gcagtttctt caggatgccg cgcatggttc
     1681 attctctccc ggtgcggttc aggttttacg gaacggtgac cgctccggct gttcgtttcg
     1741 tttgcagttc cggcaggaaa accggttccc gctttgcctt ggaaatgtcc tgggtagccg
     1801 gctctgtttt taggtatcgc cgcgttcatt taatagtgta ataaccttga tatcagtcat
     1861 catgtttgtg aattaccatg gcgccacaca agctgctcga tgttcatcca tccctgcttc
     1921 gcagctttct ggccgtggcc aacagcttga gcttcacgac cgccggcgcg cggctcgatc
     1981 ttcgccagtc caccgtcagc cagcatgtca ggcggctgga ggaggccgtc ggccagcgcc
     2041 tgctgctgcg cgacacccac agcgtcgcct tgaccgagca gggcgacgcc atggtcgaga
     2101 tggcccgcac tgttctcgat gcgaacgaca ggctggccaa tttcttcatc gacaatggaa
     2161 agcgggaacg gctgcgcctt ggaatatccg aggactttgc gatgtcgcgc cttgcaaatg
     2221 tgctgatcca gttccggcgc aacgacccgc atgtggatat cgaattgacg gtggggctgt
     2281 cgggctatct gtatcagcgc ttcgatgcgg gcgaactgga tgtgattttc gtcaagcgca
     2341 agcccggcga caagcgcggc aagaccgcct ggcgcgagcg gccggcatgg attgcccgcc
     2401 ccggcttgcg ggtgccgccc ggctcgcccg tgccgctggt ggcattctcc ccgccaagta
     2461 taacgcgctc gctggcgacg gatacgctgg acagggcggg caggccgtgg cgcatcacct
     2521 gtagcagcgg cagcatcagc gggttgcgcg cggccctgct ggcggggctt ggggtggcgg
     2581 cccattccag ccggcttatc ccgatggggc ttatgcggtt gcccgaggat gccggcctgc
     2641 cgcaactgcc cgaaatcgaa ttcgtcgcca tcggacccgg acgcacgcaa cgcctcgcca
     2701 tgcgcatggt cgatacgctc gtcgatattt cggaaagcct gcaacaggtt tgggagtaga
     2761 gcggcgcgca aaagctggac cgcctagccg agatgctgct gaatggaagc tgcgatggcg
     2821 cataaatccg cgtcccggtc gcgaaagccc atcagctgga tgccgaccgg caggccttcg
     2881 aaggtcagaa gcggcagcga cagcgcggga acgccgagat aggcgcacgg cgttgcaaag
     2941 ccggggtcgc cggtcgatgc gaggccgatg ggcgcggggc cgttggccga aagcgtgatg
     3001 caggcatcca cgccttcgtc gagaagagct gcatagatag cgcggccgcg ctcgcgctcc
     3061 agaatgccgc ggcgataatc gtctatcgtc atctgctcgg catctgcgag cctctggcgc
     3121 atgccggcgc tgagcttggc ttcatcggcg tcgcggtaga tattcagggg ccaacgcgat
     3181 tcccatgtga cgatgatgcg cgtgaccgac agggcggacg agagcgtgtt ctccacggca
     3241 tcgacgccat ggctatcaca gccgacgaca tcgataccga gcgtggccag ccggtcgagc
     3301 cgattttgga agagatcgcg caatggctgt gcaacctcgt tccagccggc ggtcttgagg
     3361 aaggccagct tggccggcat tcgagccgcc ggcagatcgt cggggcc
//
LOCUS       DASYCS010000335         7924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3137837,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000335 DASYCS010000000
VERSION     DASYCS010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 7924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7924
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3137837"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="VGM46_10505"
     CDS             <1..505
                     /locus_tag="VGM46_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006206232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEY4193063.1"
                     /translation="TTIFVTHDQEEALSISDRIAVMYGGKAEQVGTPFEIYNRPTTKF
                     VANFVGTLNVLEGTVTDAASGKVRVNGEEVALKGKLNGSSTGDMLSLALRPEAISLGR
                     QPGRDASISGRISEVHFLGSVIRVRVGIGDSTVSLDTFNSPMTPPPAVGEKAEICFSA
                     DDMLVLH"
     gene            684..839
                     /locus_tag="VGM46_10510"
     CDS             684..839
                     /locus_tag="VGM46_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193064.1"
                     /translation="MKLVSHTIQHGVGYDLERVARPQADIVPDHFPSHIDIDLETPST
                     GVSRGVR"
     gene            836..1681
                     /locus_tag="VGM46_10515"
     CDS             836..1681
                     /locus_tag="VGM46_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY4193065.1"
                     /translation="MTLLVFTAVLAAAVMHASWNALIKVRVDRFASISLAALGMACTA
                     LPVLPFVAVPAANVWPWIIGSLTIHIGYRLFLILAYEFGDLAQTYPLARGTAPLITTL
                     GGIALVSEVPGPLAIIGIFLLSAGTFLMSLRGSAQLGRLNGAAVGYALVTSLFIAGYT
                     LADGIGARLAETPIGYAAWLFVCDGVCAMTIGFFYRGRRLLAILAGEWEVGALTGFLS
                     ATAYAIVMWAMTKAPIASVASLRETSILFAMAISVLALGEKMTAWRGAAALTILAGVI
                     ALRLG"
     gene            1995..2840
                     /locus_tag="VGM46_10520"
     CDS             1995..2840
                     /locus_tag="VGM46_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006467488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY4193066.1"
                     /translation="MAFAPRTTMLAIAMLAGGPAFAADLYVEEAPEATPAPVVAVSGW
                     YLRGDIGYVFKSKSQGNWDFYNQFPGVQGIDDTLRYDHLNLGASASFGGGVGYRFNDY
                     LRTDATLDFFHAKVSGGTACPSYVRNDAAHGFLPFSDCHYDNNASANVWAPMVNAYVD
                     LPSMGAITPYLGLGIGAAFVDYGKLNVQEVCGDCPAGFSPYSATNEGLSSWRLAGSAM
                     AGLSYRVSDHFLLDAGYKYTRVGSGKAFAYDAHDAAHGATGTQIRDNGFNFHALRVGV
                     RYEVN"
     gene            complement(3188..3262)
                     /locus_tag="VGM46_10525"
     tRNA            complement(3188..3262)
                     /locus_tag="VGM46_10525"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3228..3230),aa:Val,seq:gac)
     gene            complement(3471..3833)
                     /locus_tag="VGM46_10530"
     CDS             complement(3471..3833)
                     /locus_tag="VGM46_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002966536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY4193067.1"
                     /translation="MARILLAEDDDDMRRFLVKALERAGYHVSDFDNGASAYERLREE
                     PFSLLLTDIVMPEMDGIELARRATEIDPDLKVMFITGFAAVALNPDSKAPKDAKVLSK
                     PFHLRDLVNEVEKMLQAA"
     gene            3998..4939
                     /locus_tag="VGM46_10535"
     CDS             3998..4939
                     /locus_tag="VGM46_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HEY4193068.1"
                     /translation="MARSADDLPVLASGFPVKTAAEDFSDIAPFEVRASAEQRVPFVF
                     SSPHSGRHYPERFLAMARLDHTAIRRSEDCYVDELFASAVPLGAPMLAANFPRAYLDV
                     NREPWELDPRMFSEPVPSYCNTRSARVAGGLGTVPKLVGEGLDIYPGRLPLEEAVARI
                     ETVYKPFHETLKRLLTATHARFGFAVLIDCHSMPASIRVGDNGARPDFIIGDRFGTSA
                     SAMLTDAALDLLNAMGYSVTYNKPYAGGFITEHYGRPARHLHALQVEVNRGLYMDERT
                     FKRSAGFDALADDLSRFCAGLMALPDHYFLDLPQAAE"
     gene            5007..5783
                     /gene="hisN"
                     /locus_tag="VGM46_10540"
     CDS             5007..5783
                     /gene="hisN"
                     /locus_tag="VGM46_10540"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HEY4193069.1"
                     /translation="MDISSEFMRRIAQAAAAETLPRFRRQGAVSNKEQGSFDPVTEAD
                     REAERAIRALINAEFPGHGILGEEHGSENMDSRHVWVIDPIDGTRAYISGLPVWGTLV
                     GLTVDGDAVAGMMSQPFTGELFYANASGSHYEGPGGPRKLDTRKTTRLADATLFTTTP
                     ALFKGKESRARYDSLESRVQLARYGVDCYAYAMLASGCVDIVVDPGLKPYDIVALIAV
                     IEKAGGVVTTFEGGPAEKGGDILAASTLELHAAAMAVLRG"
     gene            complement(5892..6851)
                     /locus_tag="VGM46_10545"
     CDS             complement(5892..6851)
                     /locus_tag="VGM46_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY4193070.1"
                     /translation="MTDIFHEIADNPTPEKATAGFFTARDGRKIRYAHFTATGQPSAG
                     TVVILPGRNESIEKYFETIRDLSDRGLGSAMIDWRGQGLSDRLTRDGQRGHVDSFSDY
                     AGDLDQFFGEIVLPDCHGPYYILAHSAGALIALLAAPFMANRVRRMVLIAPFLALPGQ
                     PVSMKTVYRATSLLCLLGLGRMYAAWGPRKEGGTPFAENKVTSDARRYRRNTLLYETY
                     PQLALGGPTVKWLRAASRASQTVCAPDFVSAIKVPTLIVAAGADRVVSTRTVEAYAAR
                     LRLGSLLTIDGAAHEILQEADLYREQFFAAFDAFIPGTDDPSL"
     gene            7293..7760
                     /locus_tag="VGM46_10550"
     CDS             7293..7760
                     /locus_tag="VGM46_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HEY4193071.1"
                     /translation="MRHVDFSPLYRSTVGFDRLFTILDSLAQPEGAQTYPPYNIEKTG
                     EDAYRISMAVAGFSDDEISIEAHRNVLTVKGERKEEANEQGSELLYRGIAGRAFERRF
                     QLADHVDITGAVLKNGLLYVDLKRNIPEELKPRKIAITSASATAKQIEAKAAA"
BASE COUNT         1443 a         2409 c         2482 g         1590 t
ORIGIN      
        1 caccaccatc ttcgtcacgc acgaccagga agaggcgctt tcgatttcgg atcgcatcgc
       61 ggtgatgtat ggcggcaagg ccgagcaggt cggcacgccg ttcgagattt ataaccgtcc
      121 gaccaccaag ttcgtcgcca atttcgtcgg cacgctgaac gtcctggaag gcaccgttac
      181 cgacgccgcc tccggcaagg tgcgcgtcaa cggcgaggaa gtcgccctca aaggcaagct
      241 caacggttcg agcaccggcg atatgctgtc gctggcgctg cggcccgagg cgatttcgct
      301 tggccgccag cctggccgcg acgccagcat ttcgggccgg atttccgagg ttcatttcct
      361 tggctccgtt atccgggtcc gggtgggtat cggcgacagc acggtgtcgc tcgatacgtt
      421 caacagcccg atgaccccgc cgccggcggt tggcgaaaag gcggaaatat gtttttccgc
      481 cgacgacatg ctggtgctgc actagcatcg gactgccggc cgggacgcgc aaaggcttgc
      541 gtgccgcgcg agcctttcag cagttctttg ctgctgacgg ccaagcgcca agccttgccc
      601 tggcccaagc ctgatcggta gcgggtggat tgccacgcgg gtgccgaacg gttacgtcgt
      661 taccgacatt gagccggtta acgatgaagc ttgtcagcca tactatccag cacggcgttg
      721 gctacgattt ggagcgcgtg gcgaggcccc aagcggatat cgtcccggat cattttccgt
      781 cgcacatcga tatcgacctg gaaacgccat cgaccggtgt gtcgcgcggt gtccgatgac
      841 gcttctcgtc tttaccgccg tattggccgc ggccgtcatg catgcctcat ggaacgcgct
      901 catcaaggtg agggtggacc ggttcgcttc catatcgctg gcagcgctcg gcatggcctg
      961 cacggcattg ccagtgctgc ctttcgtggc ggtgccggcg gcaaatgtgt ggccatggat
     1021 aattggctcc ctcaccattc atatcggcta caggctgttc ctcatccttg cctatgaatt
     1081 tggcgacctt gcgcagacct atccgctggc tcgcggcacg gcgccgctca tcaccacgct
     1141 gggcggcatc gcgcttgttt cggaagttcc cgggccgttg gcgataatcg gcattttcct
     1201 tctttccgcc ggcacgttcc tgatgtcgct tcgcggcagc gcgcaacttg gccggctgaa
     1261 cggcgcggcc gtcggctatg cgctggtaac gtcgctgttc atcgccggct atacgctggc
     1321 cgacggcatc ggcgctcggc ttgccgaaac gccaatcggc tatgccgcat ggctgttcgt
     1381 ttgcgacggc gtctgcgcca tgacgatcgg cttcttctat cgtggccggc gtttgcttgc
     1441 gatcctcgcg ggggaatggg aggtcggggc gctgactgga tttctcagcg ccacggcata
     1501 cgccatcgtc atgtgggcga tgacgaaagc gccgatcgca tcggtcgcat cgctgcgtga
     1561 aacgtcgatc ctgtttgcga tggccatttc cgttctggcc ctcggcgaga agatgaccgc
     1621 atggcgcggc gctgccgcat taaccattct ggccggcgtg atcgcgctgc gacttggatg
     1681 agcggcggcg aaagttccct cggcagcggc agttcacctc cgacaacaag cggaataatt
     1741 ccgcaagacg gtcgagcctt ccggaaaggc gggcggttga agcctgcgcg cgtcggcctg
     1801 ttcgaagcaa caatcggacc gatgtgtata gaagtccagt tgatgcgcat ctgtgcccat
     1861 cggcagacag attggtaaac gtcattgaaa atattcaaca ttagcttcac cggttattaa
     1921 ccatggctgt ggatagtgca tggacgagcg ccgtggtggc gttcgttccc gccatctgca
     1981 catgggtgac cgaaatggct tttgcaccac gtactacaat gctcgctatc gcgatgctgg
     2041 ccggcggccc cgcttttgct gccgatctat atgttgaaga agcgccggag gcgacgcctg
     2101 cgcccgtcgt cgccgtctcg ggatggtatt tgcgcggcga tattggttac gtcttcaaat
     2161 cgaagagcca gggcaactgg gatttctaca accagtttcc gggtgtccag gggatcgatg
     2221 acacgcttcg ctacgatcac ctgaatctgg gtgcctcggc aagcttcggc ggcggtgtcg
     2281 gctaccgctt caacgattat cttcgcacgg acgccacgct ggacttcttc cacgccaagg
     2341 tttccggcgg aacggcatgc ccttcctacg tcaggaacga tgcagcccat gggttcctgc
     2401 cattttcgga ttgtcactac gacaataatg cctcggccaa tgtgtgggcg ccgatggtca
     2461 acgcctatgt ggacctgccg agcatggggg cgatcacgcc ttatctcggc ctcggcatcg
     2521 gcgccgcctt cgtcgattat ggcaagctca acgtacagga ggtttgcggc gattgcccgg
     2581 caggcttcag cccttactcg gcaaccaatg aaggcttgag cagctggcgg ctggcgggtt
     2641 ccgccatggc cggcctgtcc tatcgcgtgt ccgaccactt cctgctggac gccgggtata
     2701 aatacacccg cgtcggcagc ggcaaggcgt ttgcctacga tgcccatgac gccgcgcacg
     2761 gcgccaccgg cacccagata cgcgacaacg gcttcaattt ccacgcattg cgcgttggcg
     2821 tgcgatacga ggtcaactga ccgcttttcg aaactcaacg cgcccggctc catgccggtc
     2881 gcggacaacg gcccaggcca tgcctgggcc gttttgcgtc aagccggtga tgcaggcgac
     2941 ctgctcgcca aggcgatgcg cgctcgcgcg ccgccatcgg cccctgttcc gtcgcgaata
     3001 tcatcgacaa ttatgcgccg cgccgccgcg ccgtggacga agagtatcag gagcggcaag
     3061 cccggttggc cgcgctgttc gcggaactgc cggcgtgaat accgcaatgg ctaccaaaag
     3121 ggcctgtcac ccacttgtcg ttttgccccg caggacccgc aaagccttgt attgtgggag
     3181 gttcggatgg tgggcgtgac agggattgaa cctgtgaccc ctgcaatgtc aatgcagtgc
     3241 tctcccgctg agctacacgc ccatccgacg ccgggcttac accacttttg gtccggcgcg
     3301 tcaatagcgg taggcccgaa acaagcgctc catttcgcgg cgtgtccggt tgcgggccta
     3361 cgacgtcgtc tgaaaaatca gaatcgattt tcgccaggca tgaaggtgcg ggtaaaaggg
     3421 gcgaggagcg cggtttgtgc gtgcagttaa acgcggctgc cctactggct tcaggcggct
     3481 tgcagcatct tctcgacctc gttgacgagg tcgcgcagat ggaagggctt cgacagcacc
     3541 ttggcatcct tgggcgcttt ggaatccggg ttgagcgcaa ccgccgcgaa gccggtgatg
     3601 aacatcacct tcaggtccgg atcgatttcg gtggcgcggc gcgccagttc gatgccgtcc
     3661 atttccggca tgacgatgtc cgtcagaagc agcgaaaaag gttcctcgcg cagccgctcg
     3721 taggcgctgg cgccattgtc gaaatcgctg acatgatatc ccgcgcgctc cagcgccttg
     3781 accaggaagc ggcgcatatc gtcgtcgtct tccgccagaa gaatgcgtgc catcgtttcc
     3841 cgtccgaatc acccgtccaa gactgccgca ggacagtccc gttaaccatc gtgtatatga
     3901 agctttgacg gtaaagatca agtgaatcac gacggaggca gtcggtctgg ccgttcgcgg
     3961 aaaacgccct cgaaatactg gacatgcgcg gcctgcgatg gcacggtcgg cggatgattt
     4021 accggtgctt gcgagtggat ttccagtgaa gacggcagcc gaggattttt cggacatcgc
     4081 gcctttcgaa gtccgtgcca gcgccgagca gcgcgtgccc ttcgtgttca gctcgccgca
     4141 tagcggccgc cactatcccg aacggttcct tgccatggcc aggctggatc acacggcgat
     4201 ccgccgttcc gaagattgct atgtcgatga attgttcgcc agcgccgttc cgctcggcgc
     4261 gccgatgctg gcggccaatt ttccccgcgc ctatctcgac gtcaaccgcg agccgtggga
     4321 actcgacccg cgcatgtttt ccgagccggt gccctcctac tgcaatacgc gctcggcacg
     4381 ggtcgcgggc gggcttggaa ccgttccgaa gctggtgggc gaggggctgg atatctatcc
     4441 ggggcgtttg ccgcttgaag aggcggtcgc ccgcatcgaa actgtctaca agccgttcca
     4501 tgaaacgctg aagcggcttt tgacggcaac ccatgcccgc ttcggcttcg cggtgctgat
     4561 cgactgtcat tccatgccgg ccagcatacg cgtcggcgac aatggagcgc ggccggattt
     4621 catcataggc gaccgtttcg gcacatcggc atcggcgatg ctgaccgatg ccgcgctcga
     4681 ccttctcaac gccatgggct acagcgtgac ctataacaag ccttacgcgg gcgggttcat
     4741 aaccgagcat tatggccggc ccgcacggca tctgcatgcg ttgcaggtcg aggtcaatcg
     4801 cggtctctat atggacgaac ggacgtttaa gcggtcggcg ggcttcgatg cgctggccga
     4861 cgatctgtcc cgcttttgcg ccgggttgat ggcgttgccc gaccattatt tcctcgatct
     4921 tccccaggcc gccgaatagt tttcgcaaag cacccggcgg aagcaacctt gccggcatgg
     4981 cttcgaacgg cattcaggag acaagattgg atatttcctc ggagttcatg cggcggatag
     5041 cgcaggccgc cgccgccgag accctgccgc gttttcgcag gcagggcgct gtctccaaca
     5101 aggagcaagg cagcttcgat ccggtcaccg aagcggaccg cgaggccgaa cgggccatcc
     5161 gcgcgctgat caatgccgag tttcccggcc atggcatttt gggcgaggag catggcagcg
     5221 agaacatgga cagccggcat gtctgggtca tcgatcccat cgacggcacg cgcgcctata
     5281 tttccggcct gccggtgtgg ggaacgctgg ttggcctgac ggtggacggc gatgcggtcg
     5341 ccggcatgat gtcgcagcca tttacgggtg agcttttcta cgccaacgct tccggatcgc
     5401 attatgaggg gccgggcggt ccgcgaaagc tcgatacgcg caagacgaca cgccttgccg
     5461 acgccacctt gttcacgacg acgcccgcgc tgttcaaggg caaggagagc agggcgcgtt
     5521 acgacagcct ggaaagccgg gtgcaactcg cccgctatgg cgtcgattgc tatgcctacg
     5581 ccatgctggc atcgggctgc gtcgatatcg tcgtcgatcc cggcttgaag ccctatgaca
     5641 tcgtggcgct gattgccgtc atcgagaagg cgggcggcgt cgtcaccacc ttcgagggtg
     5701 ggccggcgga aaagggcggc gatatcctcg ccgcctcgac gctcgaactt catgccgcgg
     5761 cgatggccgt gctgcgcggc tagatcggtt ctggttttaa ccgaatcggt aaaaccgctc
     5821 tatctctttg ttttggccgc acctccggac gcaagacagt ttcacacttt ttgctggaaa
     5881 tgctctagcc cttacaggct cggatcgtct gtaccgggaa tgaaggcgtc gaaagcggcg
     5941 aagaactgtt cgcggtaaag gtcggcctct tgcagaattt catgggcggc gccatcgatg
     6001 gtgagcaggg agccaaggcg caggcgggcg gcataggctt ccacggtgcg ggtggagacg
     6061 acccggtcgg cgccggccgc cacgatgagc gtcggcacct tgatcgctga aacgaaatcg
     6121 ggcgcgcata cggtctggga agcgcggctg gccgcgcgca gccatttgac ggtcgggccg
     6181 ccaagcgcca gctgcgggta ggtttcataa agcagcgtgt tgcgccggta gcgccgggcg
     6241 tccgacgtca ccttgttttc cgcaaagggc gtgccgcctt ccttgcgcgg accccaggcg
     6301 gcatacatcc ggccaaggcc gagcaggcat agcagcgagg ttgcgcgata gacggtcttc
     6361 atcgagacgg gctggcccgg cagcgccagg aacggtgcga tcaacaccat gcgccgcacg
     6421 cggttggcca tgaacggcgc tgcaagcaaa gcaatcagcg ctccggccga atgcgccaat
     6481 atataataag ggccatgaca gtcgggcagc acgatttccc cgaagaattg atcgagatcc
     6541 ccggcgtagt ccgaaaagct gtcgacatgg ccgcgctggc cgtcgcgggt cagccggtcg
     6601 gacaagccct gaccacgcca gtcgatcatg gcgctgccca gtccccggtc ggaaaggtcg
     6661 cggatggtct cgaaatattt ctcgatggac tcgttgcggc cgggcaggat gacgaccgtg
     6721 cccgccgatg gctggccggt ggcggtaaaa tgcgcatagc ggattttcct gccgtcccgc
     6781 gcggtgaaaa agccggcggt ggctttttca ggcgtcgggt tgtcggcaat ctcgtggaaa
     6841 atatccgtca ttcggcgctt cggtcggttg ctggcctgtg catcgacagg cgttgcatgg
     6901 tgataggaag caaaccgccc aaaagcaatt gatctggcgg gagaaaggcc ggaagcgact
     6961 tgcggtggac ttccggcccc tgtcgatccg ggggaaggga cgttacaccc ggatcggcct
     7021 cgttgcggtg ctttgggcac cgcttgcccg ttgacgtagg tgaactctag cgccggcccg
     7081 gtgaatgcag cccgaagggg cggttcatcc ggcgttcatc gcgggattaa aggcgttcga
     7141 tcctgcgtgc cccgaaacct tgaaaccttc gccggtgaca cccaaatgtg gggagcagtc
     7201 gccgctatcg gggctgctgg cccagccgaa acgccgttga cgggtttggc aaggtcaatc
     7261 gcaaaccatg ttgctcacca ggagaacaca ccatgcgtca cgttgatttt tcccctctct
     7321 atcgctcgac cgtcggcttc gaccggctgt tcaccattct cgattcgctg gctcagcccg
     7381 agggcgcgca gacctatccg ccctataata tcgagaagac cggcgaggac gcctatcgca
     7441 tctcgatggc ggtagcgggc ttttcggatg acgagatctc gatcgaagcc catcgcaatg
     7501 tgctgaccgt taagggcgag cgcaaggaag aggccaatga gcagggcagc gagctgcttt
     7561 atcgcggcat tgccggccga gccttcgagc gccgcttcca actggccgat cacgtcgata
     7621 tcactggcgc ggtgctgaag aacggcctgc tctatgtcga cctcaagcgc aacattcccg
     7681 aggaactgaa gccgcgcaag atcgcgatta cttcggcttc cgcgaccgcc aagcagatcg
     7741 aggccaaggc agccgcgtaa cgccaggcca ggtcttctcg cgatagtaaa ggcggcgctg
     7801 aaaagcgccg tctttcattt ggcgagaagg ctgcccagcc tgtcgatatc gtcggccgtg
     7861 gtcgcgaaac tggtgacgaa acggtagagc gcctcatcct cagtcagcgg atcggtgaaa
     7921 ccgg
//
LOCUS       DASYCS010000336         2748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_237203,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000336 DASYCS010000000
VERSION     DASYCS010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237203"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VGM46_10555"
     CDS             <1..191
                     /locus_tag="VGM46_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193072.1"
                     /translation="SIAIMAISFLEICRARLDLYCEHSHRSLIATISTLPGGLLSRPV
                     RLIHVLALSGSTIGSDAQ"
     gene            complement(287..1405)
                     /locus_tag="VGM46_10560"
     CDS             complement(287..1405)
                     /locus_tag="VGM46_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HEY4193073.1"
                     /translation="MKEIVVVGAGKIGSTIAGMLAETGDYTVTIVDRAAGQLAALELS
                     ALDPSSGVTTLELDIAAEGALEKALAGKFAVMSAAPFHLTTRIAEAAANAGVHYLDLT
                     EDVASTRRVKELARDAKTAFIPQCGLAPGFISIVAADLASRFDTLESVRMRVGALPQY
                     PSNALNYNLTWSTDGVINEYCEPCEAIVEGELVEVPPLEEREEFSLDGVTYEAFNTSG
                     GLGTLAETLKGKVRTLNYRTIRYPGHAAIMKALLNDLGLRHRRELIKDIFENALPATT
                     QDVVIVFVTVSGRRNGRLMQETYANKVYSQRVGKAVRSAIQITTASGICAVLDMLADG
                     SLPATGFVRQEDIALDAFLANRFGSAYAQHEAVTRLAS"
     gene            1520..1948
                     /locus_tag="VGM46_10565"
     CDS             1520..1948
                     /locus_tag="VGM46_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012093088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY4193074.1"
                     /translation="MLSDAEQDLLALLRENARASTAELARRLGVSRTTVQSRIERLEA
                     RGVIGGYGVRLSRDYERGLVRAHVLLTVTPKLADKVVRELKALPGVRTLHSVSGNFDM
                     IVVVESPSISDLDALLDSIGAMDGVERTLSSIILSTRIDR"
     gene            2088..>2748
                     /locus_tag="VGM46_10570"
     CDS             2088..>2748
                     /locus_tag="VGM46_10570"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEY4193075.1"
                     /translation="MSKLKDVKKVVLAYSGGLDTSIILKWLQTELGAEVVTFTADLGQ
                     GGELEPARKKAEMMGIKDIRVMDVREEFVKDYVFPMFRANAVYEGVYLLGTSIARPLI
                     SKHLVDIAAETGADAIAHGATGKGNDQVRFELSAYALNPDIKVIAPWRDWTFKSRTDL
                     LNFAEQHQIPVPKDKKGDAPFSVDANLLHSSSEGKVLEDPWSEPPEFVHQRTISPMDA
                     PD"
BASE COUNT          507 a          808 c          886 g          547 t
ORIGIN      
        1 tttcgattgc cattatggca atttcctttc tggaaatttg ccgggcaaga cttgacttgt
       61 actgcgaaca ctcgcaccgt tcgctcattg caacaatttc caccttaccc ggcgggcttc
      121 tgtcaaggcc agtacgtctg atacacgtgc tggccttatc cggttcgacc atcggttcgg
      181 atgcgcaatg attccagaaa tctattcatg tgcaaataca atatgtggct gaagcccgtg
      241 agtagagcga gaacatagct acatattgtg gattaagtgg ataagttcag cttgccagcc
      301 gcgtcaccgc ctcgtgctgg gcgtaggcgc tgccgaagcg gttggcgagg aaggcgtcga
      361 gcgcgatgtc ttcctggcgc acgaagccgg tcgccggcag gctgccatcg gccagcatgt
      421 cgagcaccgc gcagatgcct gacgccgtgg taatctggat ggcgctgcgc accgccttgc
      481 cgacgcgctg gctgtagacc ttgttggcat aggtttcctg catcaggcgg ccattcctgc
      541 ggccggagac ggtgacgaag acgatgacca cgtcctgcgt cgtcgccggc agggcatttt
      601 cgaaaatgtc cttgatcaac tcgcggcggt ggcgcaagcc aaggtcgttg agcagcgcct
      661 tcatgatggc ggcgtggccg ggatagcgga tggtgcggta gttcagcgtg cgcaccttgc
      721 ccttcagcgt ttcggccagc gtgccgaggc cgcccgatgt gttgaacgcc tcataggtta
      781 cgccgtcgag cgagaattcc tcgcgttcct ccagcggcgg cacttcgacc aactcgcctt
      841 cgacgatggc ctcgcacggt tcgcaatatt cgttgatgac gccgtccgtg ctccaggtca
      901 ggttgtagtt cagcgcgttg gacgggtatt gcggcagcgc gccgacgcgc atgcgcacgc
      961 tttccagcgt atcgaagcgg ctggcgagat cggcggcgac gatggagatg aagccgggcg
     1021 ccaggccgca ttgcgggatg aaggccgtct tggcgtcgcg cgccagttcc ttgacgcggc
     1081 gcgtcgaggc gacgtcctcg gtcaggtcga gatagtgcac gccggcgttc gcggcggctt
     1141 cggcgatgcg cgtggtcagg tggaaggggg cagcgctcat gacggcgaac ttgccggcca
     1201 gcgctttttc cagcgcgcct tcggcggcga tgtcgagttc gagcgtcgtt acgccactgg
     1261 aagggtcgag ggcggaaagt tcgagggcgg ccagctggcc tgccgcgcgg tcaacgatgg
     1321 tgacggtata gtcgccggtt tccgccagca ttccagcgat ggtcgagccg atcttgcccg
     1381 ctccgacgac gacgatttcc ttcatgaccc gctccctttg cctgttggcg cagcattgca
     1441 gcttgcctga cgaaaggaac cgtgcgatcc gtcgaaacaa cgccctgttt tgatcataat
     1501 gccgatgaaa gccgtcataa tgcttagcga tgccgaacaa gacctgctgg cgctgttgcg
     1561 cgaaaacgcc cgcgcatcga cagcggaact ggcgcgaagg ctgggcgtgt cgcgcactac
     1621 ggtgcagagc cgcatcgagc ggctggaggc gcggggcgtc atcggcggct atggcgtgcg
     1681 gctgtcgcgc gattacgagc gggggctggt gcgggcgcac gtgcttttga cggtaacgcc
     1741 gaagcttgcc gacaaagtgg tgcgcgaact caaggcgctg ccgggggtca ggacgctgca
     1801 ttcggtcagc ggcaatttcg acatgatcgt ggtggtcgaa tcgccctcga tcagcgatct
     1861 ggatgcgttg ctcgacagta tcggggcgat ggatggcgtc gagcgcacgc tgtcgtcgat
     1921 catcctgtcg acgcggatcg accgctagaa ccgtctggaa aagcgacgct ttccaaacgg
     1981 tctggagctg gcttgtcggc gcatcccctt gcggtaaccg gaaatggcgt gatacctcgc
     2041 ccggaaccct atcatgccca accccctacc acggaacggc gaaaacaatg tccaagctca
     2101 aagacgtgaa aaaggtcgtg ctcgcctatt cgggcggtct cgacacctcc atcatcctca
     2161 aatggcttca gacggagctt ggcgcggagg tggtgacctt caccgccgat ctcggccagg
     2221 gcggcgagct ggagccggcg cgcaagaagg ccgagatgat gggcatcaag gatatccgcg
     2281 tcatggatgt gcgcgaggag ttcgtgaagg actatgtctt tccgatgttc cgcgccaatg
     2341 ccgtctatga aggcgtctat ctgcttggca cctcgattgc gcggccgctc atctccaagc
     2401 atctggtcga tatcgccgcc gagacgggag ccgacgccat cgcccatggc gcgaccggca
     2461 agggcaacga ccaggtccgc ttcgaacttt ccgcctatgc gttgaacccc gacatcaagg
     2521 tcatcgcgcc gtggcgcgac tggacgttca agtcgcgcac cgatttgctg aatttcgccg
     2581 aacagcacca gattccggtg ccgaaggaca agaagggcga tgcgccgttt tcggtggacg
     2641 ccaatctgct gcactcttcc tcggaaggca aagtgctgga agacccgtgg agcgagccgc
     2701 cggaattcgt gcatcagcgc accatttcgc cgatggacgc acccgatg
//
LOCUS       DASYCS010000337         8277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2556586,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000337 DASYCS010000000
VERSION     DASYCS010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 8277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8277
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2556586"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..1243)
                     /locus_tag="VGM46_10575"
     CDS             complement(98..1243)
                     /locus_tag="VGM46_10575"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEY4193076.1"
                     /translation="MQAPFALFRDDLAGREVLFEQPSDIIVAHTADEFEQALACVQAA
                     HQSGKWLAGFFSYEAGYLLEPKLKPLLPEGRRAPLLCLGVFDGPSDRICEQASQRATN
                     GPIFDAKAAWSFEDYEKRFASLHRHIRQGDCYQGNLTFPVHAHWSGAPDAAFDALTER
                     QPVRYGALLNLCEPIVLSRSPELFFEIDAQGVIEAHPMKGTAPRGATEAEDAALKEFL
                     RNDGKNQAENRMIVDLLRNDISLISEVGTLEVPELFRIETYPTVHQMVSRVRAKLPPD
                     LTIRQIFAALFPCGSITGAPKIRAMEILRDLEETPRDVYCGAIGWIAPGGRMRFSVAI
                     RTLSLFAGGDAVYNVGGGIVFDSTVEEEYRECLLKARFATGSPPAST"
     gene            complement(1282..3129)
                     /locus_tag="VGM46_10580"
     CDS             complement(1282..3129)
                     /locus_tag="VGM46_10580"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006338899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEY4193077.1"
                     /translation="MRVVSVRQAAASPTGPNTQERAAALGDLPEWNLGDLYSGMEAPE
                     LKRDLARAASDAADFEKRWKGTLATEAGRGAAGKLGEALQAYERMEDLIGRIVSYAGL
                     LYAGDTSDPQRAKLYGDIQEKMTDASSHLLFFGLELNLIDNAVIANALDADPVLGHYR
                     PWVLDLRKDKPHQLDDRIEQLFHEKSITGRGAWNRLFDETMTELRFDIDGEALTLEPT
                     LNRLQDADGDVRRKASEALAATFRENLRTFTLITNTLAKDKEISDRWRGFEDIADARH
                     LANRVERPVVDALASAVRDAYPRLSHRYYAMKARWLGMDVMNHWDRNAPLPETPRATI
                     SWGQAKDTVLTAYNRFSPEMADIARTFFDRRWIDAPVRPGKSPGAFAHPTVPSVHPYV
                     LLNYMGKPRDVMTLAHELGHGVHQVLAGDQGALMASTPLTLAETASVFGEMLTFRSLL
                     DQTADRRERKAMLAQKVEDMINTVVRQIAFYEFERKVHTERKNGELTSDKLGQFWLEV
                     QAESLGPAIKLREGYETFWTYVPHFIHSPFYVYAYAFGDCLVNSLYAVYQNAERGFQD
                     KYFAMLRAGGTKHHSELLAPFGLDATDPAFWQIGLGVIGSLIDELEALD"
     gene            3347..4879
                     /locus_tag="VGM46_10585"
     CDS             3347..4879
                     /locus_tag="VGM46_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006338897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY4193078.1"
                     /translation="MTGSILIVDDDPVQRRLVEAAVTKFGHVAVVAESGEAGLKAMER
                     RGISVVILDLVMPGLDGIDVLKAMRERGIDIPVIVQTAQGGIETVVLAMRHGAFDFVV
                     KPASPDRLNASIGNALKVEAVEDEVKRAARSGGGHLTFKDMVTQSPAMDRVIRLGQKA
                     AASNIPILIEGESGVGKELVARAIQGLGDRRSKPFVTVNCGAIPDNLVESILFGHEKG
                     SFTGATEKHTGKFVEAHSGTLFLDEIGDLPLDVQVKLLRAVQDGEVDPVGGRSTVKVD
                     IRLISATHRNLLERVKDGAFREDLFYRLNVYPIFVPPLRERRSDIPHLVRHFLEKFGP
                     AGSRARPNGISDTALAMLQAYDWPGNIRQLENAVFRACVLAEGDMLGEDEFPQIRAQV
                     EGVVRLDDAPETDRPEAEAFEPGVVIGTVGDGSIGNGLAARPAPAISRSGKLDAIDER
                     GNIRSLAAVELEMIKLAIDHYNGQMSEVARRLGIGRSTLYRKLKEYGIDPETGRMERL
                     AS"
     gene            5219..7051
                     /locus_tag="VGM46_10590"
     CDS             5219..7051
                     /locus_tag="VGM46_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020629034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HEY4193079.1"
                     /translation="MVAALGFLALTSGGASAETRSLKFYHLHTHEKAEIVYKRNGRYD
                     AVGLKKINFILRDWRRDEPVKIDPRLLDLVWEAYRATGSHDYIQVICGYRAPATNSML
                     RSRSRGVAEKSQHMLGKALDFYIPGVPLKKLRDIGLKMQGGGVGYYPTSGSPFVHMDV
                     GNVRHWPGISRQELVRVFPNGKTLHVPSDGRPLPGYEQALAAYKSRKSSGTTAIALAS
                     TGSSSKSTNLLAALFGGGADEEEDVADVEMAAAAPVKPVRVAKKAPASGGSLPGIEIV
                     SPENAHRTEVPQVAVASADDAQPEMEAMLPEKIVAALPMRSVPLPAFAPRPKAEVGVQ
                     VAEASTDQPEMVPFETASARMPAPDGVPFPAAAAAPSDEAVEVAVNNIPLPSWRPKTA
                     PAELNVPQDQKSVLMALADTAENGNSASDALSILPSERPDVVAVSAAGDDLAKQDRAQ
                     RDEVRAVLEHVSTDDYQVASLTQAPLPRSAFNDPSGVDAASPRTAVATRLSGSDPASA
                     IGAGVKTTRKEARANLGDQRPEPKAVVVSAEPKVARWALHSADYVATVKDSTTAPRFA
                     YNLVRTAPSEVYTNGFQSDYEMANANRFTGTAVKFLSVARFQTK"
     gene            complement(7139..7780)
                     /locus_tag="VGM46_10595"
     CDS             complement(7139..7780)
                     /locus_tag="VGM46_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006338890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEY4193080.1"
                     /translation="MPSKTEKLLSLLDRQPVIPVLKIADIDHAVPLARALARGGLPMI
                     EITLRTAKALEAIRRVAAEVEEAIVGAGTVLNAGQFDQAAKAGSKFVVSPGITRELLA
                     AADGSAVPLLPGAITPGEIMTAYEAGLNFLKFFPAEQAGGVAFLKALASPFADIKFCP
                     TGGINVGNATDYLGLPNVICVGGSWVAPDDLVSAGKWDAIEALARAASTLGNR"
     gene            complement(7856..>8277)
                     /locus_tag="VGM46_10600"
     CDS             complement(7856..>8277)
                     /locus_tag="VGM46_10600"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HEY4193081.1"
                     /translation="AEALRNHDWIAFAKGYNGPAFAQNAYDRKIATAYARYATTGGDG
                     ASRLRLGSKGAAVVDLQRNLTALGYPLAADGDFGPATTQAVMRFQRDRRLAADGIVGA
                     ATAGALQNTASPFNPLARIASNAWAWFTGWLARFKPS"
BASE COUNT         1538 a         2487 c         2695 g         1557 t
ORIGIN      
        1 tggcgaaacc ccaactcggc cgcggaagcg gcgagccggg cgagatggcg atcgagccgc
       61 acgaagccgt cgcccggctg ccagcgcatg gtttcgatca ggtcgaagcc ggcgggctcc
      121 ctgtggcgaa ccgggctttc agcaggcact cgcgatactc ctcctcaacc gtggaatcga
      181 agacgatgcc gccgccgaca ttgtagacgg cgtcgccgcc ggcaaagagc gaaagcgtgc
      241 ggatggctac cgaaaaacgc atccggccgc ccggcgcgat ccagccgatg gcgccgcaat
      301 agacgtcgcg cggcgtttct tcgaggtcgc gcaggatttc catggcgcgg attttcggcg
      361 cgccggtgat cgagccgcag ggaaacaatg ccgcgaatat ctgtcggatg gtaagatcgg
      421 gcggcagctt ggcccgcacg cggctgacca tctgatggac ggtcggatag gtctcgatgc
      481 ggaaaagctc gggaacttcc agcgtgccga cctcgcttat cagcgaaatg tcgttgcgca
      541 gcaaatcgac gatcatgcgg ttttccgcct ggttcttgcc gtcattgcgc aggaattcct
      601 tgagtgccgc atcttcggcc tcggtcgcgc cgcgcggcgc ggttcccttc atcggatgcg
      661 cttcgatgac accttgcgcg tcgatttcga agaaaagctc gggcgagcgc gacaggacta
      721 taggctcgca caggttgagc aacgcgccgt atctgacagg ctggcgctcg gtaagcgcgt
      781 cgaaggccgc gtctggcgca ccggaccagt gggcatggac gggaaaggtc aaattgccct
      841 gatagcagtc gccctggcga atgtggcgat gcaggctggc aaaacgtttt tcgtaatcct
      901 cgaaagacca tgccgccttc gcatcgaaga tcggaccgtt ggtggcgcgc tgtgacgctt
      961 gctcgcaaat gcgatccgac ggcccgtcga aaacgccgag acagaggagc ggtgcgcggc
     1021 gcccctccgg caagagcggc ttcagcttgg gttccagcag ataaccggcc tcataggaga
     1081 aaaagccggc cagccatttg cccgattggt gggcggcctg cacgcaggcc aaggcctgct
     1141 cgaactcatc ggcggtgtgc gcgacgatga tatccgacgg ttgctcgaac aggacctcgc
     1201 ggccggcgag gtcgtctcta aacagggcga agggcgcttg catcatcaag cagtatcagg
     1261 aaatcgcctt gccgggctgt ctcaatccag cgcttccagt tcgtcaatga ggctgccgat
     1321 gacgccaagg ccgatctgcc agaaggcagg gtcggtagcg tctaagccga agggggccag
     1381 cagttctgaa tgatgcttgg tgccgccggc gcgcagcatg gcgaaatact tgtcctgaaa
     1441 gccgcgctcg gcattctggt agacggcata aagcgagttc accaggcaat cgccgaaggc
     1501 ataggcatag acgtagaacg gcgaatgaat gaagtgcgga acataggtcc agaacgtctc
     1561 gtagccttcg cgcagcttga tcgccgggcc aaggctttcg gcctgcacct ccagccagaa
     1621 ctggccaagc ttgtccgagg tcagttcgcc gttcttgcgc tcggtatgca ccttgcgctc
     1681 gaattcgtag aaggcgatct ggcgcacgac cgtgttgatc atgtcctcga ccttctgcgc
     1741 cagcatcgcc ttgcgttcgc gcctgtcggc ggtctggtcg agcagggagc ggaaggtcag
     1801 catttcgccg aagacggaag cggtttcagc cagcgtcagc ggcgtcgagg ccattagcgc
     1861 gccctggtcg ccggccagca cctgatgcac gccatggcca agttcatggg caagcgtcat
     1921 cacgtcgcgc ggcttgccca tgtaattcaa cagcacgtag gggtggaccg aaggcacggt
     1981 cgggtgagcg aaagcgccgg gcgattttcc cggccgtacc ggcgcgtcga tccagcggcg
     2041 gtcgaagaag gtgcgcgcaa tatcggccat ctccggagaa aagcggttat aggcggtcaa
     2101 aaccgtatcc ttggcctgac cccaggagat cgttgcccgc ggcgtttcgg gcaggggggc
     2161 gttgcggtcc cagtggttca tcacatccat gcccagccag cgcgccttca tcgcataata
     2221 gcggtgcgac aggcggggat aggcatcgcg cacggccgag gccagcgcat cgacgacggg
     2281 acgctcgacc cggttggcga ggtggcgcgc gtcggcgatg tcttcgaagc cgcgccagcg
     2341 gtcggagatt tccttgtcct tggccagcgt gttggtaatc agcgtgaagg tgcgcaggtt
     2401 ttcgcggaag gtggcggcga gcgcttcgga agccttgcgg cgcacatcgc catcggcatc
     2461 ctgaagccgg ttgagcgtcg gctccagagt caatgcctcg ccgtcgatat cgaaacgcag
     2521 ttccgtcatc gtctcgtcga acagacggtt ccaggcgccg cgcccggtaa tcgatttttc
     2581 gtggaaaagc tgttcgatgc ggtcgtcgag ctggtgcggc ttgtccttgc gcaggtcaag
     2641 cacccagggc cggtagtggc ccagcaccgg gtcggcatcc agcgcatttg caatgacggc
     2701 attgtcgatc aggttgagtt caagcccgaa gaaaagcaga tgcgagctgg cgtcggtcat
     2761 cttctcctga atgtcgccat agagcttggc gcgctgcggg tccgaggtgt cgccggcata
     2821 gagcaggccg gcataggaaa cgatgcggcc gatcaagtct tccatccgct cataggcttg
     2881 caaagcttcg cccaacttgc ctgccgcgcc gcgcccggcc tcggttgcga gcgtgccctt
     2941 ccagcgcttt tcgaaatcgg cggcatcgct tgccgcgcgc gccaggtcgc gcttcagttc
     3001 cggggcttcc atccccgaat agagatcgcc aaggttccat tcaggcaggt cgccaagcgc
     3061 ggcggcgcgc tcctgcgtgt tgggtccggt gggggacgcc gccgcctggc ggactgaaac
     3121 tacgcgcatt gccaaatacc ttgtcgaatc ttgcggccta gccttggggc cgaactttgg
     3181 gggctgattt gggatggaat ttccggatcg aataatgttg ccgaagagaa aggccggtca
     3241 aatcctaagg aattcattca ccggcttttt tcaaagctta tttagaaccc tgtgccagga
     3301 tgatccatag ggcaaacttc ctcgcgcggg caaattcgaa ccactcatga caggttcaat
     3361 cctcatagtc gatgacgatc cggtgcagcg ccggctggtt gaggcggcgg taacgaagtt
     3421 cggccatgtg gcggtcgtgg ctgagagcgg cgaagccggt ctcaaggcca tggagcggcg
     3481 cggcatttcg gtggtcatcc tcgatctcgt catgccgggc ctcgacggca tcgacgtttt
     3541 gaaggcgatg cgcgaacgcg gcatcgacat tccggtcatc gtgcagaccg cgcaaggcgg
     3601 catcgaaacc gttgttctgg ccatgcgcca tggcgctttc gatttcgtcg tcaagccggc
     3661 ctcgccggac cggctcaatg cgtcgatcgg caacgcgctc aaggtcgagg cggtggaaga
     3721 cgaggtaaag cgcgcagcaa ggagtggcgg cggtcacctg accttcaagg acatggtgac
     3781 gcaaagcccg gcgatggacc gggtgatccg tcttggccag aaggctgccg cgtcgaacat
     3841 tccgattctg atcgaaggtg aatcgggcgt cggcaaggaa ttggtggcgc gcgccatcca
     3901 gggcttaggc gaccgccgca gcaagccctt cgttaccgtc aattgcggcg cgatcccgga
     3961 caatctggtg gaatcgatcc tgtttggcca cgaaaaagga tcgttcaccg gcgctaccga
     4021 aaagcatacc ggcaaattcg tcgaggcgca ttcgggcacg ctgtttctgg acgagatcgg
     4081 cgacctgccg ctcgatgtgc aggtgaaact tttgcgggcc gtgcaggatg gcgaggtcga
     4141 tccggtcggc ggccgctcga ccgtcaaggt cgatataagg cttatctcgg cgacgcaccg
     4201 caacctgctg gaacgggtca aggacggcgc tttccgcgag gacctttttt accggctgaa
     4261 cgtctatccg atcttcgtgc cgcccctgcg cgagcggcgc agcgatatcc cgcatctggt
     4321 tcggcatttc ctggaaaaat tcgggccggc cggttcccgc gcccgtccaa acggcatttc
     4381 cgacacggcg ctggccatgc ttcaggccta tgattggccg ggcaatatcc ggcagctgga
     4441 aaacgcggtt ttccgggctt gcgtgctggc cgagggggac atgctgggcg aagacgaatt
     4501 tccgcagata agggcgcagg tagagggcgt ggtgcgcctg gatgatgcgc cggaaacgga
     4561 ccgcccggaa gccgaagcct ttgagcccgg cgtggtcatc ggcacggttg gcgacgggtc
     4621 gatcggcaat gggttggccg cgcgtccggc gcctgccatt tcgcgttcag gcaagctcga
     4681 tgcgatcgac gaacgcggca atatccgctc gcttgcggcg gtggagcttg aaatgatcaa
     4741 gctcgccatc gaccattata acgggcagat gagcgaagtc gcccggcggc tcggcatcgg
     4801 gcgttcgacg ctttaccgga agctcaagga atatggcatc gatcccgaaa ctggccgcat
     4861 ggagaggctc gcttcctgat cccgcgccgg gcgaccgggt tgaggcttgt tcacgcattg
     4921 tggcgtgcgg taacagatcg tgctcctgtg gcggcgaaag atcatctaaa ccaacagttt
     4981 actatgattc tccgctgcgg gttttcgccg ggttatcgcc atggtgttac cgcatttgag
     5041 cttcaataag cgatgattaa ccattaggat cgatattcgg atgtagtttc gccacatccg
     5101 tttgggcaaa tccggggaca aacggcagcc tgtagggcct tttgatttga acagaatcga
     5161 acgacacacg aacgggcggc agtttcctac gggcgtctgg cctaaatggc tggtgtcgat
     5221 ggttgccgcc ttggggttcc tggcgctgac ctcgggtggc gcttccgccg agacgcggtc
     5281 gctgaaattc taccacctgc atacgcatga gaaggccgag atcgtctaca agcgcaatgg
     5341 ccgctacgac gctgttggcc tcaagaagat caatttcatt ttgcgcgact ggcgccgcga
     5401 cgaaccggtc aagatcgatc cgcgtttgct ggatctggtg tgggaagcct accgcgccac
     5461 cggctcgcac gactatatcc aggtcatttg cggctatcgc gcgccggcga ctaactcgat
     5521 gctgcgcagc cgcagccgtg gcgtcgccga aaagagccag catatgctcg gcaaggcgct
     5581 ggatttctac attcccggcg ttccgctcaa gaaactgcgc gatatcggcc tgaagatgca
     5641 gggcggcggc gtcggctatt accccacttc cggctcgccc ttcgtgcaca tggatgtcgg
     5701 caatgttcgc cattggcccg gcatcagccg ccaggaactc gttcgcgttt tcccgaatgg
     5761 caagacgctg catgtgccat ccgatggccg gccgctgccc ggctatgagc aggcgctggc
     5821 cgcctataag tcgcgcaaga gcagcggcac cacggctatc gcgctggcca gcactggttc
     5881 ttccagcaag tcgaccaatc tgcttgccgc cctgtttggc ggcggcgccg acgaggaaga
     5941 agacgtagcc gatgtcgaga tggcggccgc ggctccggtg aagccggttc gcgtggcgaa
     6001 gaaggctccg gctagcggcg gcagcctgcc gggcattgaa attgtatcgc ctgagaatgc
     6061 gcatcgcacc gaggttcccc aggtggccgt cgcttccgcc gatgacgcgc agcctgagat
     6121 ggaagcaatg ctgccggaaa agatcgtggc cgcgctgccg atgcgcagcg ttccgctgcc
     6181 ggcctttgcg ccgcgtccaa aggcggaggt cggcgtccag gtggccgagg cctcgacgga
     6241 tcagcctgaa atggtgcctt tcgagacggc ttccgcgcgg atgcctgcgc ccgacggcgt
     6301 tcctttcccc gccgccgccg ccgcgccgtc cgatgaggcg gtggaagttg ccgtcaacaa
     6361 tattcctttg ccaagttggc gtccgaagac ggcgccggcc gaactcaacg ttccgcagga
     6421 ccagaaatcg gtgctgatgg cgcttgccga tacggctgaa aacggcaatt cggccagcga
     6481 cgcgctgtcc atactgcctt cggaaaggcc cgacgtggtt gcggtttctg ccgccggcga
     6541 tgatttggcg aagcaggacc gggcgcagcg cgatgaagtc cgcgcggtgc tggaacatgt
     6601 ttcgaccgat gattatcagg tggcttcgct gacgcaggcg ccgctgccgc gctctgcctt
     6661 caacgatccg tcgggtgtcg atgcggcgtc gccgcgcaca gccgtggcaa cgcgcctgtc
     6721 cggctccgat ccggcttcgg cgatcggcgc cggcgtcaag acgacccgca aggaagcccg
     6781 cgccaacctc ggcgaccaga ggccggaacc gaaggcggtc gtcgtctcgg ccgagccgaa
     6841 agtggcccgg tgggcgctgc acagcgccga ctatgtcgcc actgtcaagg attccaccac
     6901 cgcgccgcgc tttgcctata atctggtccg caccgcgcca agcgaggtct ataccaacgg
     6961 cttccagtcc gattacgaaa tggccaacgc caaccgtttc accggcacgg cggtgaaatt
     7021 cctgtcggtg gctcgtttcc agaccaaata atttcgaagc atcatgaaaa agggccgcgc
     7081 cagattgcgc ggcccttttt tgtttcgtgg cgggaatgcc gccaaatagc cggccggctc
     7141 agcgattgcc cagcgtgctg gccgcgcggg ccagcgcctc gatggcgtcc catttgccgg
     7201 cgctgacgag atcgtcgggc gcgacccagg agccgccaac gcagatgacg ttgggcaggc
     7261 cgagatagtc ggttgcgttg ccgacattga tgccgccggt cggacagaac ttgatgtcgg
     7321 cgaagggcga ggcgagagcc ttcagaaagg ccacgccgcc cgcctgctcg gctggaaaga
     7381 atttaaggaa gttcaggccg gcttcatagg cggtcatgat ttcgccgggc gttatcgcgc
     7441 cgggcagcag cggcaccgcg ctgccatcgg cagcagcgag aagttcgcgc gtgatgccgg
     7501 ggctgacgac gaatttcgac ccggcctttg ccgcctgatc gaactggccg gcattgagga
     7561 cggtgccggc gccgacgatt gcctcttcca cttcggcggc aacccgccgg atggcttcca
     7621 gcgctttcgc ggttcgcagc gtgatttcga tcatcggcag gccgccgcgc gccaacgcgc
     7681 gcgccagcgg caccgcgtgg tcgatatcgg cgatcttcaa cactggaatt accggctggc
     7741 ggtcaagcag cgacaaaagc ttttcggttt tgctgggcat ttcgatcttt caggaaaaag
     7801 ggagtttcaa ccgattagca tatgggcaat ttcctgtcca cgaagacggg cgcgctcagc
     7861 tgggcttgaa ccgcgccaac catccggtga accacgccca tgcattcgag gcgatgcgcg
     7921 ccagcgggtt gaaaggcgag gcggtatttt gcaaagcgcc ggcggtggcg gctccgacaa
     7981 tgccgtccgc cgccagccgg cgatcgcgct ggaagcgcat caccgcctgc gtggtggccg
     8041 gcccgaaatc accgtctgcc gcaagcggat agccaagcgc ggtcagattg cgctgcaagt
     8101 cgacgacggc tgcgcctttc gatcccaggc gcagccggga agcgccatcg ccgccggtcg
     8161 tcgcatagcg cgcataggcg gttgcgatct tgcggtcata tgcgttttgg gcgaaggccg
     8221 ggccgttata accttttgcg aaggctatcc agtcgtgatt gcgcagggct tcggcca
//
LOCUS       DASYCS010000338         6151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4150681,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000338 DASYCS010000000
VERSION     DASYCS010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 6151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6151
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4150681"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VGM46_10605"
     CDS             complement(<1..278)
                     /locus_tag="VGM46_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018431021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY4193082.1"
                     /translation="MIEPISTTPFGRRSLSLAMVAGQMAAKRCAPGASANKWQVFRAV
                     CEAKLALGASDRALGVLSALLSFHPDADLVEGEGLVVFPSNAQLALRA"
     gene            complement(538..1659)
                     /gene="repB"
                     /locus_tag="VGM46_10610"
     CDS             complement(538..1659)
                     /gene="repB"
                     /locus_tag="VGM46_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning protein RepB"
                     /protein_id="HEY4193083.1"
                     /translation="MAGQDRKNQLKALFQTMETPAADHGAQPQPAAPQTPSPQTPAQQ
                     APLAQPSMSSAFPPAGEARSRAASGAVKAMGLSLGGLSREVEDARRLKESLGAADRVV
                     EIEPERIDGSFINDRLGHDDSDAGFQALMRSIEASGQQVPVLVRPHPEKSGRFQTAYG
                     HRRIRAAAALGQRVRAIVRELSDTELVLAQGKENNERRDLSFIERAFFARALMARGFE
                     RGVVQEALSLHKAEMTRYLQVADAVSLRVVNAIGPAPKIGRPRWMALADLLKGEAAQV
                     KAEDEITGARFRAASGSDARFNVLFARLQRKPAKDGHAREVRDGEGRPVAQISVAKGK
                     ARVEFVDKIADGFEDFLGQELPKLLERFRQGASGGAERK"
     gene            complement(1671..2963)
                     /gene="repA"
                     /locus_tag="VGM46_10615"
     CDS             complement(1671..2963)
                     /gene="repA"
                     /locus_tag="VGM46_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014762401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning protein RepA"
                     /protein_id="HEY4193084.1"
                     /translation="MDILPSGEVFDLASSSLQTAGLKQSGADSAGWSTDRAIARDAEM
                     LSAQLQAIRDRLYAPSASKALRRFSSGEAARLIGVSDGYLRQLSIAGDGPQPETGAGG
                     RRFYSLEDINGLRRHLAEQNPAKARTYLPHRDAARGEHLQVIAVTNFKGGSGKTTTSA
                     HLVQHLALAGYRVLAIDLDPQASLSALFGYQPELDLDGNDTLYAAIRYDDESRPLADI
                     IRKTYFDGLDIVPGNLELQEFEHTTPQMLAARERDDGTGGPMFFARVQAALASVADRY
                     DVVVMDCPPQLGFLTLSALCAATSVLVTVHPQMLDVASMSQFLFMTADLLSVVRAAGG
                     ELNFDFMRYLVTRYEPNDGPQTQIVGFLRSLFGERVLTAPLVKSTAFSDAGLTKQTLY
                     EVSRESFSRATYDRAMESLTAVNSEIEGLIMKAWNRPA"
     gene            3314..3556
                     /locus_tag="VGM46_10620"
     CDS             3314..3556
                     /locus_tag="VGM46_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HEY4193085.1"
                     /translation="MPEDRAPPILLHRVDPARNMARFYGLSLQPTLFGEISVVREWGR
                     IGTRGRIKIDSYPTGEGATAAFTRLERSKRRRGYRG"
     gene            complement(3576..5372)
                     /gene="ade"
                     /locus_tag="VGM46_10625"
     CDS             complement(3576..5372)
                     /gene="ade"
                     /locus_tag="VGM46_10625"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018428712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HEY4193086.1"
                     /translation="MIEPWFQSGPRLVEVAMGRAPADMVVRNVRWVNVHSGEIIPDTD
                     IAIAAGRFAYVGPDASHAIGPQTETIDGAGRYALPGLCDAHMHVESGMVTVSEFARAV
                     IPHGTTSMFIDPHEIANVLGLAGVRLMHDEAAGLPINVFVQMPSCAPSAPGLENTGFS
                     LGPAEVREAMAWPNIIGLGEMMNFPGVVAGNADMLAEIAATQAAGKTVGGHYASKDLG
                     LPFHAYVAAGPADDHEGTSVEDCVARVRQGMRAMLRLGSAWYDVAAQIKAVTERGLDS
                     RSFVLCTDDSHSGTLVGEGHMNRVVRHAIAQGLKPVTAIQMATLNAAQHFGMERELGS
                     ITPGRRADLILTGDLVDLPVELVVARGQVMARDGRLVSEIAPYDYPASAKDTVKLGKK
                     LAAADFDIAAPAGANQVTARVIGVVENQAPTRALEASLGVANGVVQIDRANDICQIAL
                     VERHRATGQVTNAFVSGFGYDKPCALASTVAHDSHHMIVVGTDKADMALATNRLGEVG
                     GGVVLFSEGRELALVRLPIAGLMSDQRAEIVAEQANALMAAMRACGCSLNNAYMQHSL
                     LALVVIPELRISDIGLIDVLKFQPIPVLVETA"
     gene            5715..>6151
                     /locus_tag="VGM46_10630"
     CDS             5715..>6151
                     /locus_tag="VGM46_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193087.1"
                     /translation="MKRRRGAPARQALAAAMVLILAGGAFCGIDAKAQQQPDAAGQGS
                     STPAAAPIAEPNSSAPAMQPASSIQQAPRTQPAPLAPSTTPIRPTPLAQPVPPAQPAA
                     NIQPAPSTLPAPSTLPAPASGAQQPAAVAQPAPGGVSDNAPNI"
BASE COUNT         1086 a         2085 c         1942 g         1038 t
ORIGIN      
        1 tgcgcgcgca acgccagctg tgcattggac gggaacacca ccagcccctc cccttccacg
       61 agatcggcat cgggatggaa gctcaacagc gcgctcaaga cgccgagcgc ccggtcgctg
      121 gcgccgagcg ccagcttggc ctcgcagacg gcgcgaaaga cctgccattt attggcgctg
      181 gcgccgggcg cgcagcgctt tgccgccatc tgccccgcca ccatggcaag cgacagcgat
      241 cgccgcccga agggcgtcgt cgagataggc tcgatcatct tcgttcacct caataaaagg
      301 caagccgaag gcattccgga acgcgtccag tgttgacaag tcggtccgga aatgattctc
      361 tcacaattgc tacattgatg agggcttccg gaatggtgac gtttcggggg cctttttctt
      421 gccggtctat ccttctgttt cctgttcgct acgcattggg cactaacgct ctacgctttt
      481 tcgccgccgg cgaattgagc gcaaacaatg cgcctgcctg gccggccgtg gataaattta
      541 tttccgttcg gccccgccgc tggccccctg cctgaacctt tccaacagct tgggcagttc
      601 ctggcccaga aaatcctcga aaccatcggc tattttatcc acgaattcga cgcgggcctt
      661 gcccttggcg acgctgatct gcgcgaccgg cctgccctcg ccgtcgcgaa cctcgcgcgc
      721 atggccatcc ttggccggct tgcgctgcaa gcgcgcaaac aacacgttga agcgcgcatc
      781 gctgccggaa gcggcgcgga agcgcgcccc ggtgatctcg tcttcggcct ttacctgcgc
      841 cgcctcgccc ttcagcagat cggccagcgc catccatctc ggccggccga tcttgggggc
      901 ggggccgatg gcgttgacga cgcgcaacga caccgcatcg gcgacttgca ggtagcgcgt
      961 catttccgcc ttgtgcagcg acagcgcctc ctgcacgacg ccgcgctcga agccgcgcgc
     1021 catcagcgca cgggcgaaga aggcgcgctc gatgaaggaa agatcgcgcc gctcgttgtt
     1081 ttccttgccc tgcgccagca ccagttcggt atcgctaagc tcgcgcacga tggcgcgcac
     1141 tcgctggcca agtgcggcgg cggcgcgaat gcggcggtgg ccataggcgg tctggaagcg
     1201 gccggatttt tccggatgcg gccgcaccag caccggcacc tgctggccgc tggcctcgat
     1261 gctgcgcatc agcgcctgga aacccgcatc gctgtcgtca tggccgagcc ggtcattgat
     1321 gaacgacccg tcgatgcgtt ccggctcgat ctcgacgacg cggtcggcgg cgccaaggct
     1381 ttccttcagc cggcgcgcat cctcgacctc gcgcgacagg ccgcccagcg aaagccccat
     1441 ggccttgacc gcgccggaag ccgcgcggct gcgcgcctcg ccggccggtg gaaacgccga
     1501 cgacatggat ggctgcgcca aaggtgcctg ttgcgcaggc gtctgcggcg agggtgtctg
     1561 cggcgcagcc ggctgcggtt gcgcgccatg atcggcggcg ggcgtttcca tcgtctggaa
     1621 aagcgccttc aactggttct tgcggtcttg tcctgccatt cctgcctcct tcacgccggc
     1681 ctgttccagg ctttcatgat aaggccttcg atctcactgt tgacagctgt caacgattcc
     1741 atcgcccggt cgtaggttgc gcggctgaag ctttcgcgcg aaacctcgta taatgtctgc
     1801 ttggtcagcc cggcgtcgga aaatgctgtc gacttgacca gcggcgcggt cagcaccctc
     1861 tccccgaaca gcgagcgcag gaagccgacg atctgcgtct gcggcccatc attcggctcg
     1921 tagcgggtga cgaggtagcg catgaagtcg aaattaagct cgccgcccgc cgcgcgcacc
     1981 accgacagca aatccgccgt catgaacagg aactggctca tcgaggcgac gtcgagcatc
     2041 tgcggatgga cggtgaccag caccgaagtc gccgcgcaca gcgccgacag cgtcagaaag
     2101 ccaagctgcg gcggacaatc catgacgacc acgtcatagc ggtcggcgac cgaggcgagc
     2161 gccgcctgca cgcgggcaaa gaacatcggc ccgcccgtgc cgtcatcacg ctcgcgcgcc
     2221 gccagcatct gcggcgtggt gtgctcgaat tcctgaagtt cgagattgcc gggcacgatg
     2281 tccagcccgt cgaaataggt cttgcggatg atgtccgcca gcggccggct ctcgtcgtca
     2341 tagcgaatgg cggcgtagag cgtgtcgttg ccgtcgagat cgagttccgg ctggtagccg
     2401 aacagcgccg aaagcgaagc ctgtggatcg aggtcgatgg ccagcacccg gtaaccggcc
     2461 agcgccagat gctgcaccag atgcgcgctg gtggtggtct tgccggaccc gcccttgaaa
     2521 ttggtgaccg cgatgacctg caaatgctcg ccgcgcgccg cgtcgcggtg cggcagatag
     2581 gtccgcgcct tggccgggtt ctgttcagcc agatgccggc gcagcccgtt aatatcctcc
     2641 agcgaataaa aacggcggcc gcccgcgccc gtctccggtt gcgggccgtc gccggcaatc
     2701 gaaagctggc gcagatagcc gtccgaaacg ccgatcagcc ttgccgcctc gccgctggaa
     2761 aaacggcgca gcgccttgct ggccgacggc gcatagagcc ggtcgcgaat ggcctggagc
     2821 tgggccgaca acatttcggc gtcccgcgcg atggcgcggt ccgtcgacca gccggcgctg
     2881 tcagcgccag actgcttcag accagccgtt tgcaggcttg aagacgccaa gtcaaaaacc
     2941 tctcccgatg gtaaaatgtc catcccgtct tccttatgcg ccgattcgcc ggagattccg
     3001 aatcccgctt acgccccctc gaaagtttac ggggacaagc ggagataacg cgcgcggcga
     3061 tcagttacga taaacatggc gagatacgcc aatctccgta aatgatgggc gggcttgcga
     3121 gtcgggtcaa gcactttata gttaaccgaa attaactttt ctgccgccca gtggcgatct
     3181 ggccggccgg gttgacagct gtcaaccgcg cgaccacgca ggccgccaat ccatcaatgc
     3241 catgaatcca tccgcgacga ccgattcata ataggcgttg gacgccgcag ccggcatcga
     3301 ttctaaccac cacatgccag aagaccgcgc cccgcccatc ctgcttcacc gcgtcgaccc
     3361 ggcccgcaac atggcgcgct tctacgggct gtcgctgcaa ccgacgctgt ttggcgagat
     3421 ttccgtggtg cgcgaatggg gccgcatcgg cacgcgcggc cgcatcaaga tcgattccta
     3481 cccgacaggc gagggcgcga ccgccgcctt cacccgcctc gaacgctcga agcgccggcg
     3541 cggctatcgc ggctaggttt tgtcgcgata aaagctcacg cggtttcgac cagcaccggt
     3601 atcggctgga acttcagcac gtcgatcagc ccgatatcgg agatgcgcag ttcggggatg
     3661 accaccagcg ccaacagcga gtgctgcata taggcgttgt tgaggctgca cccgcaggcc
     3721 cgcatcgccg ccatcagcgc attggcctgc tcggcgacga tttcggcgcg ctggtcggac
     3781 atcaggccgg cgatgggcag gcgcaccagc gccagttcgc ggccttcgct gaacagaacg
     3841 acgccgccgc cgacctcgcc aaggcggttt gtcgccaacg ccatatcggc cttgtcggtg
     3901 ccgaccacga tcatgtggtg gctgtcatgg gcgaccgtcg aggccagcgc gcagggcttg
     3961 tcgtagccaa agccggaaac gaaggcgttg gtgacctggc cggtggcgcg atggcgctcg
     4021 accagcgcga tctggcagat atcgttggcg cggtcgatct ggacgacgcc gttggcgacg
     4081 ccgagcgaag cctccagcgc gcgggtaggg gcctggttct cgacgacgcc gatgacccgc
     4141 gccgtcacct ggttggcgcc ggcgggcgct gcaatgtcga agtcggcggc agcaagcttc
     4201 ttgcccagtt taacggtatc cttggccgag gccggatagt cgtaaggcgc gatctcgctg
     4261 accagcctgc cgtcgcgcgc catcacctgg ccgcgcgcga cgaccagttc caccggcaga
     4321 tcaacaagat cgccggtcag gatgaggtcg gcgcggcgtc cgggtgtgat cgaacccagt
     4381 tcgcgttcca tgccgaaatg ctgggcggcg ttcagcgtcg ccatctggat ggcggtgacc
     4441 ggcttcagcc cctgcgcgat ggcgtggcgc acgacgcggt tcatatggcc ctcgccgacc
     4501 agcgtgccgg aatggctgtc gtcggtacat agaacgaagc tgcgcgaatc gaggccgcgt
     4561 tcggtgacgg ccttgatctg ggcggcgaca tcgtaccagg ccgaaccaag ccgcagcatg
     4621 gcgcgcatgc cctggcgcac ccgcgccacg caatcctcga cgctggtgcc ttcatggtcg
     4681 tcggcggggc cggcggcgac ataggcatgg aagggcaggc cgagatcctt cgaggcataa
     4741 tggccgccga ccgtcttgcc cgccgcctgc gtggcggcga tttccgccag catgtcggcg
     4801 tttccggcaa cgacgccggg aaaattcatc atctcgccaa ggccgatgat attcggccat
     4861 gccatcgctt cgcgcacttc ggccgggccg aggctgaagc cggtattttc gaggccgggc
     4921 gccgacggcg cgcagctcgg catctgcacg aacacgttga tcggcaggcc ggccgcctcg
     4981 tcatgcatca gccgcacgcc ggccaggccc agcacattgg cgatttcgtg cgggtcgatg
     5041 aacatcgagg tggtgccgtg cgggatgacg gcgcgcgcga attcgctgac ggtgaccatg
     5101 ccgctctcga catgcatatg cgcgtcgcaa aggccgggca gggcgtagcg ccccgcgccg
     5161 tcgatcgtct cggtttgcgg tccgatggcg tgcgaggcat ccggcccgac ataggcgaaa
     5221 cgcccggccg cgatggcgat gtcggtgtcc ggtatgatct cgcccgaatg cacgttcacc
     5281 cagcgcacat tgcgcaccac catgtcggcg ggcgcgcgac ccatggcgac ttcgacaagg
     5341 cgcgggccgc tctggaacca gggttcgatc atcgttcatc ctctcttcag ccgcgcgccg
     5401 gttgggctgt gtcgcccgat gcggcactgc atcgccgacg aagctagcag ggcagggcga
     5461 aatccgcgag cgccgcaaag ccgcaccgca ccgcaatgcg atgcaatgcg acaaatggcg
     5521 ggaaacttcg ccgaaggtcc cggcttaaac ttgaacattt ttatcaacaa tgataatcag
     5581 cctgctgaac gccggggcga agcgcgatgg cgcgttcgcc ggaagcggcg gggaaaacgc
     5641 agcgcgccgg gcgaccgcag gctcctcaat tctggaacga ccgaggcatt aaaggcatga
     5701 acggctccat cacaatgaag cggcgccgcg gcgcaccggc aaggcaggcg ctggccgccg
     5761 cgatggtgct gatacttgcc ggcggcgcgt tttgcggcat cgacgccaag gcgcaacaac
     5821 agcccgacgc ggcagggcag gggagttcga cccccgccgc cgcgccgata gccgagccga
     5881 attcatccgc gcccgccatg caaccggcat cgtctatcca gcaagcgccc cgcacacagc
     5941 ctgcgccgct tgccccgtca accacgccaa tccggccaac accacttgcc cagccagtgc
     6001 cgccggcaca gcctgctgcc aacattcagc ctgcgccctc caccctgcct gcgccctcca
     6061 ccctgccagc gcccgcgtcc ggcgcgcagc aacccgccgc agtcgcgcag ccagcgcccg
     6121 gcggcgtctc ggataacgcg ccaaacatta a
//
LOCUS       DASYCS010000339         5336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_5167746,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000339 DASYCS010000000
VERSION     DASYCS010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 5336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5336
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5167746"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="VGM46_10635"
     CDS             <1..816
                     /locus_tag="VGM46_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEY4193088.1"
                     /translation="GALGALGGFAGDAGNVGRHLALVAGTSSCVMAMSSEPQPFAGVW
                     GPYFGVALPGLWLSEGGQSATGALLDHVIRWHGAGGEPDARTHAGIAARIMELRATEG
                     LDLAGRLHVLPDFHGNRSPFSDPHALGVISGLTLDASFDSLCRLYWRTAVAIALGVRH
                     VLEALNENGYLIDTLHVTGGHTRNPLLMELYADATGCTVVEPLEDEAMLVGTAMVAAT
                     AAGLHADLNAAGIAMQQRTRTRPPNPTAAGRFDRDYRIFLEMHRQRQVLDGIV"
     gene            986..2383
                     /locus_tag="VGM46_10640"
     CDS             986..2383
                     /locus_tag="VGM46_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007009742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEY4193089.1"
                     /translation="MSATEADASAPGNPWHAEIRATLALSWPMVLTNLAQTAMTVTDV
                     MILGRVNADTLAAGALGSNLYFAPMVFGLGLMLATMPMMAREVGRNRHSVRDLRRTVR
                     QGLWAAVFIAIPIWLLLWHGEMILLAMGQQPALAAAAGAYLRFMQWGLLPFYFYVVLR
                     SFITTLERPGWATVVAFVAVGVNLAGNWVLVFGHLGLPRLGIVGAGIATSLASLTMFL
                     GMVLVVCLDRRFRRYRLFGRFWRADWPRFRQLMRIGLPIAAMMGFETTLFNAAAFLMG
                     VIGASSLAAYAIVIQLCSVSFMVPLGVNQAATVRVGIAYGAGDASGVSRAGWTAYGVG
                     VGFMAVSALVMIAMPRLLIGLFIDTDNAANTEVVAIAATFLIFAALFQIVDGAQAVGA
                     GMLRGLHDTGVPMMIAAIGYWGVGMPLGAVLAFHFGFAGVGIWIGLSAGLAVVAVLLL
                     LRWLRRDRLLPFHPA"
     gene            2537..3841
                     /gene="pncB"
                     /locus_tag="VGM46_10645"
     CDS             2537..3841
                     /gene="pncB"
                     /locus_tag="VGM46_10645"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005977689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0019358 - nicotinate nucleotide salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEY4193090.1"
                     /translation="MARTDIARRVYNHTWKLDPIVRSLLDTDFYKLLMLQMIWGMYPK
                     VDATFSLINRTTSVRLADEIDEGELREQLDHARTLRFSKKEMIWLGGNNFYGRKQIFE
                     PEFLAWLAKFQLPEYHLNRHDGQYELTFSGPWMYTTLWEIPALAIINELRSRAAMKVY
                     GPFALDVLYARAKAKMWAKTDLLKMLPDINISDFGTRRRHSFLWQRWCVEALKEGIGT
                     AFTGTSNVLLAMDNDLEALGTNAHELPMVFAALANSEQELVQSPYKVLQDWQRYYGGN
                     LLIVLPDAFGTAAFLRDAPDWVADWTGFRPDSAPPIEAGEKIISWWREKGRDPKQKLL
                     VFSDGLEVETIVEAYRHFRGKVRMAFGWGTNLTNDFEDCAPVPTNRLDAISLVCKVTK
                     ANGRPAVKLSDNPEKATGDPAEIARYLRIFGEKDRARHLVKV"
     gene            4035..>5336
                     /locus_tag="VGM46_10650"
     CDS             4035..>5336
                     /locus_tag="VGM46_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015314468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY4193091.1"
                     /translation="MPDTKGLLGLSKAALSRRTALKGIAATAGLITAPGFVRYSQAQT
                     SAPVRIGFQSHRTGIGAAYGHWYEKTTAAAIKAINDAGGINGRPVEVVIEDDGTDAGR
                     GAEVVAKFAKQHKTDIVYGTLFSNVVVGSAPTAGELKIPYYVVSEGYHIASGKMNRWC
                     LQPGITDVRAQVTSMAPWIAANAGKKITMIFPDYAFGYDHRDYLPPALEKAGAKVVAQ
                     IAIPPTESSFTKYFPQIPADTDVIYHVMVGPAVLTFVKELGEFYGSSGPKLFGFIDSL
                     EAVDIDSPGLEYLEGSHFWEGSPRFAQANDSEGQKAYRAAVGINDQGAAIDNPKDVST
                     AGHMFGCWETLYVIKKAMEDCAYKGPEDRARLVEATEALTTFAEGPEHPQGPKTFNGK
                     LHQVFGVQYISKLEAGRLNVVHRTAIEDGLYESETDYTKMAL"
BASE COUNT          910 a         1701 c         1671 g         1054 t
ORIGIN      
        1 ggcgcgcttg gcgcgcttgg cggtttcgcc ggcgatgctg gaaacgtcgg acggcatctg
       61 gcgctggttg ccggcacctc cagctgcgtc atggcgatgt cgtccgaacc gcagcctttt
      121 gccggcgtgt ggggacccta ttttggcgtt gcgctgcccg gcctttggct gtcggagggc
      181 gggcaatcgg ccaccggcgc gctgctcgac cacgtcatcc gctggcatgg cgcaggtggc
      241 gagccggatg cgcgaacgca tgccggcatc gccgcgcgga tcatggagct gcgcgccacg
      301 gagggcctcg atcttgccgg acggctgcac gtattgcccg atttccatgg caaccgttcg
      361 ccattttccg atccgcatgc gctgggcgtc atcagcgggc tgacgctcga tgcctcgttc
      421 gacagcctgt gccggctcta ctggcgcacc gccgtggcaa tcgcgctggg cgtgcgccat
      481 gtgctggagg cgctgaacga aaacggctat ctgatcgata cgctgcatgt caccggcggc
      541 cacacgcgca acccgctgct gatggagctt tatgccgacg ccaccggctg caccgtggtc
      601 gagccgctgg aagacgaggc gatgctggtc ggcaccgcaa tggtggcggc gaccgctgcc
      661 ggcctgcacg ctgacctgaa cgctgccggc atcgccatgc agcagcgcac gcggacccgg
      721 cctcccaatc cgactgctgc cggaaggttc gaccgcgact accggatttt cctcgaaatg
      781 caccgtcagc ggcaggtgct ggacggcatc gtctagcgcc gccgaaccgc tttgcctgga
      841 tcaatcactc ccggccggcg ctatattgcc aaaatgtcgc gggtcggtca gaacggcact
      901 ctcatgatgc ggcaaagtaa ggcctttgcc cgtgaaatgc ggcttccggc gcctttgccg
      961 atgcgccgcc gaaagatgga cacccatgtc tgcgaccgaa gccgacgcat ctgcgccggg
     1021 aaatccctgg catgcggaaa tcagggcgac gctggcgctt tcctggccga tggtgctgac
     1081 caatctcgcc cagaccgcga tgacggtcac cgacgtcatg atccttggcc gcgtcaacgc
     1141 cgacacgctg gcggcgggcg cgctgggcag caatctctat ttcgcgccga tggtgttcgg
     1201 ccttggcctg atgctggcca ccatgccgat gatggcgcgc gaggtcgggc gtaaccggca
     1261 ttcggtgcgc gacctgcgcc gcaccgtccg ccagggactg tgggccgctg tgttcatcgc
     1321 catcccgatc tggctgctgc tgtggcatgg cgagatgatc ctgcttgcca tgggccagca
     1381 gccagcgctt gccgcagctg ccggggccta tctgcgcttc atgcagtggg gcctgttgcc
     1441 gttctatttc tatgtcgtgc tgcgttcctt catcaccacg ctggagcgtc ccggttgggc
     1501 gacggtggtg gccttcgttg ccgtcggcgt caatctggcc ggtaactggg tgctggtctt
     1561 cggccatctc ggcttgccgc gccttggcat tgtcggcgcg ggcatcgcca ccagcctcgc
     1621 cagcctgacg atgttcctgg gcatggtgct ggtagtctgc ctcgaccgcc gcttccggcg
     1681 ttaccgcctg ttcggccgtt tctggcgcgc ggactggccg cgttttcgcc agttgatgcg
     1741 gatcggcctg ccgattgccg cgatgatggg tttcgaaacg acattgttca acgcagccgc
     1801 gttcctgatg ggggttatcg gcgcttcctc gcttgccgcc tacgccatcg tcatccagct
     1861 ttgttcggtc agcttcatgg tgccgctcgg cgtcaaccag gccgcgacgg tgcgcgtcgg
     1921 cattgcctat ggcgcgggcg atgccagcgg cgtcagccgc gccggctgga cggcatatgg
     1981 cgtcggtgtc ggcttcatgg cggtttcggc actggtaatg atcgcgatgc cgcgcctgct
     2041 catcggcctg ttcatcgaca ccgacaacgc cgccaacacg gaagtcgtgg ctatcgcggc
     2101 caccttcctg atcttcgcgg cgctgttcca gatcgtcgat ggcgcgcagg cggtgggcgc
     2161 cggcatgttg cggggcctgc acgataccgg cgtgccgatg atgatcgcgg ccatcggcta
     2221 ttggggcgtc ggcatgccgc tgggcgccgt gctggccttt catttcggct ttgccggcgt
     2281 cggcatctgg atcggcctgt cggccggcct tgccgtggtc gccgtgcttc tgttgctgcg
     2341 ttggctgcgc cgcgaccggt tgctgccgtt tcacccggct tgagcgcgct cggcgtccgc
     2401 tcggccaatg tctggggctg ttgtttttgc cggctggtcg gcaaaagcgg gaagcgtttt
     2461 tccgaattcg atgctaagag gcttgccgca ggcggtcacc aagggtcaga accccgcagg
     2521 gaaggcgaca acttctatgg caagaaccga cattgcgcgg cgcgtctaca atcatacctg
     2581 gaagctcgac ccgatcgtgc gcagcctgct cgatacggac ttctacaagc ttttgatgtt
     2641 gcagatgatc tggggcatgt atccgaaggt cgacgccacc ttctccctta tcaaccgcac
     2701 cacttcggtt cgtctcgccg acgagatcga cgagggcgaa ctgcgcgaac agctcgacca
     2761 tgcgcgcacg ctgcgcttct ccaagaagga gatgatctgg ctcggcggta ataatttcta
     2821 tggccgcaag cagattttcg agccggaatt cctggcctgg ctggcaaagt tccagctgcc
     2881 cgaatatcac ctgaacagac atgacggtca gtatgagctg accttctccg gcccgtggat
     2941 gtacaccacc ttgtgggaaa ttcccgctct cgccatcatc aacgaactgc gctcgcgcgc
     3001 ggcgatgaag gtctacggcc cgtttgcgct cgacgttctg tatgcgcgcg ccaaggccaa
     3061 gatgtgggcg aagaccgacc tgctgaagat gctccccgac atcaacattt ccgatttcgg
     3121 cacccgccgc cgccactcct tcctgtggca gcgctggtgc gtcgaagcgt tgaaggaggg
     3181 catcggcacg gccttcaccg gcacgtccaa cgtgcttctg gccatggaca acgatctcga
     3241 agcgctcggc accaacgcgc atgaactgcc gatggtgttt gccgcccttg ccaattccga
     3301 gcaggagctt gtccagtcgc cctacaaggt gttgcaggac tggcagcgct attatggcgg
     3361 taatctgctg atcgtcttgc cggacgcttt cggcaccgcc gctttccttc gtgacgcgcc
     3421 cgactgggtc gccgactgga ccggcttccg ccccgacagc gcgccgccca tcgaggccgg
     3481 cgaaaagatc atttcctggt ggcgcgagaa aggccgtgac cccaagcaga aactgctggt
     3541 gttttccgat gggcttgaag tcgagacgat cgtcgaggcc taccgccatt tccgtggcaa
     3601 ggtgcgcatg gctttcggct ggggcaccaa tctgaccaat gatttcgagg actgcgcgcc
     3661 ggtgccgacc aaccggctgg atgccatttc gctggtttgc aaggtcacga aggccaatgg
     3721 ccgcccggcg gtgaagcttt ccgacaatcc ggaaaaagct accggcgatc cggcggaaat
     3781 cgcacgatat ttgcgcattt tcggcgagaa agaccgcgct aggcacctgg tgaaggtata
     3841 gctggcttcc tttacggaag gattgccggc gctggtcgcg gcttcgccat ctaacgcttt
     3901 gatcgggcat cggaataatc ggaaaaccgg tttccacttt tcggtcgatg ctctatggaa
     3961 gatgcgccgt ttcatacttt gccgccaagg cgattggcca gcaccagtaa ccagcaccag
     4021 caaaaggact tccgatgcct gacaccaagg gactgctagg cctttccaaa gcagcgctca
     4081 gccgccgcac ggcgctgaaa ggcattgccg ccaccgccgg gctgattacc gctcctggct
     4141 tcgttcgcta ttcgcaggcg cagacttccg ctccggtcag gatcggcttc cagtcgcatc
     4201 gcaccggcat cggcgcggcc tatggccatt ggtacgagaa aaccaccgcc gccgcgatca
     4261 aggcgatcaa cgatgccggt ggcatcaatg gccggccggt cgaggtcgtc atcgaggacg
     4321 atggcacgga tgcgggccgc ggcgcggaag tggtggctaa attcgccaag cagcacaaga
     4381 cggatatcgt ctacggcacg ctgttctcca atgtcgtcgt cggctccgcg ccgaccgccg
     4441 gcgaactgaa gataccctat tacgtcgtca gcgaaggcta ccacatcgca tccggcaaga
     4501 tgaaccgctg gtgcctgcaa cccggcatta ccgatgttcg cgcgcaggta acgtcgatgg
     4561 cgccgtggat cgccgccaat gccggcaaga agatcacgat gatcttcccc gactacgcct
     4621 tcggctacga tcaccgcgac tacctgccgc cggcgctgga aaaggccggc gcgaaagtgg
     4681 tggcgcagat cgccattccg ccgacggaat cttccttcac caagtatttt ccgcagatcc
     4741 cggccgacac cgacgtcatc taccatgtga tggtcggtcc ggccgtgctt accttcgtca
     4801 aggaactcgg cgaattctac ggctccagcg gcccgaagct gttcggcttc atcgattcgc
     4861 tggaggccgt cgacatcgat agccccggtc tggaatatct cgaaggcagc catttctggg
     4921 aaggctcgcc gcgcttcgcg caggccaacg acagcgaggg ccagaaggcc tatcgcgccg
     4981 ccgtcggcat aaacgaccag ggcgccgcca tcgataaccc gaaggatgtc tcgaccgccg
     5041 gccatatgtt cggctgctgg gaaacgctct acgtcatcaa gaaagcgatg gaggattgcg
     5101 cctataaggg accggaagac cgcgccaggc tggtcgaggc gacggaggcg ctgaccacct
     5161 tcgccgaagg gccggagcat ccgcagggac cgaagacctt caacggcaag ctgcatcagg
     5221 tgttcggcgt ccagtacatc tcgaagctgg aggcaggccg gctgaacgtg gttcaccgca
     5281 ccgctatcga ggacggtctc tacgaatccg aaaccgacta taccaagatg gcgctg
//
LOCUS       DASYCS010000340         2211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_382443,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000340 DASYCS010000000
VERSION     DASYCS010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382443"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="VGM46_10655"
     CDS             complement(<1..431)
                     /locus_tag="VGM46_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006325665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HEY4193092.1"
                     /translation="MILFPAIDLKDGQCVRLKLGDMATATVYNEDPAAQARVFEAQGF
                     EWLHVVDLNGAFKGESVNGAAVDAILKATKNPVQLGGGIRTLAQIETWLERGLARVIL
                     GTVAVRDPELVKQACKAFPGKVAVGIDAKGGKVAVEGWAEAS"
     gene            complement(428..1078)
                     /gene="hisH"
                     /locus_tag="VGM46_10660"
     CDS             complement(428..1078)
                     /gene="hisH"
                     /locus_tag="VGM46_10660"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002965149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEY4193093.1"
                     /translation="MRVAIIDYGSGNLRSVTKAFERAAGEAGIAAEIELTADAGRVRG
                     ADRIVLPGVGAYADCAAGLHAVEGMWEAIEEAAIAKGRPFLGICVGMQLMSHRGLEKA
                     VTTGLGWIAGDVKEITPSDPALKIPQIGWNTVEVLRDHPLFAGIPTGPGGLHAYFVHS
                     YHLDAQRPDEVLAVADYGGRVTAAVARDNLAGTQFHPEKSQALGLALIANFLRWKP"
     gene            complement(1080..1550)
                     /locus_tag="VGM46_10665"
     CDS             complement(1080..1550)
                     /locus_tag="VGM46_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019170907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HEY4193094.1"
                     /translation="MAVYVVMEPPGRGNAVDTAFMRDGFSWPGFLVPPLWLLWHRLWI
                     EAVLAFVAIGLLIALGETFDAPLSAMLLVVLVMLYVGLEGQALRVGALARRGWRQWGV
                     VVADDVEDADARYALDAGGDPAEDAPPLSMTPDPTLARPTRHGSLGLVPFSGRP"
     gene            complement(1553..2152)
                     /gene="hisB"
                     /locus_tag="VGM46_10670"
     CDS             complement(1553..2152)
                     /gene="hisB"
                     /locus_tag="VGM46_10670"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018430533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEY4193095.1"
                     /translation="MSPRSAEISRKTRETDISVSVHVDGSGKSDMATGVGFFDHMLDQ
                     LSRHSLVDMTVRAKGDLHMDDHHTVEDTGIALGQALAKALGERRGIMRYASINLAMDE
                     TLTRAAIDVSGRPFLVWNVAFSSPKIGTFDTELVREFFQALAQNAGITLHVTNHYGAN
                     NHHIAETCFKAVARVLRAALETDPRQPQAVPSTKGSLKG"
BASE COUNT          377 a          735 c          719 g          380 t
ORIGIN      
        1 gaagcttccg cccagccctc gaccgccacc ttgccgccct tggcgtcgat gccgacggcg
       61 accttgccgg ggaacgcctt gcaggcctgc ttcaccagtt cgggatcgcg caccgccacc
      121 gtgccgagga tgacgcgggc aaggccgcgc tccaaccacg tttcgatctg cgccagcgtg
      181 cggatgccgc cgccaagctg caccgggttc ttcgtcgcct tcaggatggc gtcgaccgcc
      241 gcaccattga cgctctcacc cttgaaggcg ccgttgaggt cgacgacgtg cagccattcg
      301 aagccttgcg cctcgaacac cctcgcctgc gctgccgggt cttcgttgta gacggtcgct
      361 gtcgccatgt cgccaagctt gaggcgcacg cactggccgt ccttgaggtc tatggcgggg
      421 aacaggatca cggcttccac ctgaggaaat tggcgatgag agcgaggccg agcgcctggc
      481 tcttttcggg atggaactgc gtgccggcaa ggttgtcgcg cgcgaccgcc gccgtcaccc
      541 ggccgccata gtcggcgacg gccagaacct cgtccggcct ttgcgcgtcg agatgataag
      601 aatgaacgaa ataggcgtgc agcccgcctg gcccggtcgg aatgccggca aagagcggat
      661 ggtcacgcag gacttcgacc gtgttccagc cgatctgggg gattttcaag gccgggtctg
      721 acggcgtgat ttccttgacg tcgccggcta tccagccgag acccgtggtg acggcttttt
      781 ccagcccgcg gtgcgacatc aattgcatgc cgacgcagat ccccaaaaac ggccggcctt
      841 tggcgatggc ggcttcctcg atcgcttccc acatgccttc gacggcgtgc agtccggcgg
      901 cgcaatcggc ataggcgccg acgcccggaa gcacgatgcg gtcggcgccg cgcacccggc
      961 cggcatcggc ggtcagctcg atctcggcgg cgatgcccgc ctcgccggcg gcgcgctcga
     1021 acgccttggt caccgagcgc agattgccgg agccgtagtc gatgatggca acgcgcatgt
     1081 cagggtcttc cggaaaaggg caccaggccg agcgacccat ggcgagtggg acgggcaagc
     1141 gtcgggtcgg gcgtcatgga cagcggcggc gcgtcttcgg ccggatcgcc gccggcatcc
     1201 agcgcatagc gcgcatcggc gtcttcaaca tcgtccgcga caacgacgcc ccattgccgc
     1261 cagccgcgac gggccagcgc gccgacccgc agcgcctgtc cttccagccc gacatagagc
     1321 atgacgagga cgaccagcag catggcgctc agcggcgcgt cgaatgtttc gcccaaggcg
     1381 atcaagaggc cgatggcaac gaaggcgagg accgcttcga tccacagccg atgccatagc
     1441 agccatagcg gcggcaccag gaagcccggc cacgagaagc cgtcgcgcat gaaggccgta
     1501 tcgactgcat tgccgcggcc cggcggttcc atcaccacat agacggccat gcctacccct
     1561 tcagcgatcc cttggtggat ggtacggcct gcggctggcg cgggtcggtt tcgagcgctg
     1621 cgcgcagcac gcgcgccacc gccttgaagc aggtttcggc gatgtggtgg ttgttggcgc
     1681 cataatggtt ggtaacatgc agggtgatgc cggcattttg cgccagcgcc tggaagaact
     1741 cacgcaccag ctcggtgtcg aacgtgccga tcttgggcga ggagaacgcc acgttccaga
     1801 ccaggaacgg ccggcccgaa acatcgatgg cggcgcgcgt cagcgtttcg tccatggcaa
     1861 ggttgatcga ggcatagcgc atgatgccgc gccgctcgcc cagcgccttg gccagcgcct
     1921 ggccaagcgc gatgcccgta tcctcgaccg tgtggtgatc gtccatatgc aggtcgccct
     1981 tggccctcac cgtcatgtcg accagcgaat ggcgcgacag ctggtctagc atgtggtcga
     2041 agaagccgac gcctgtcgcc atgtccgatt tgcccgaacc gtcgacatgg accgacacgg
     2101 aaatatcagt ttccctggtc ttgcggctga tttcggcgga acggggcgac atggccttgt
     2161 ccttgtccca gagtttagat gcccgacgtt tcgatgtccg gcgtttcgat c
//
LOCUS       DASYCS010000341         7538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2848730,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000341 DASYCS010000000
VERSION     DASYCS010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 7538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7538
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2848730"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="VGM46_10675"
     CDS             complement(<1..476)
                     /locus_tag="VGM46_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HEY4193096.1"
                     /translation="MPSKGAVFVLSGENARLSEVAAACDPAGTLERAFPVAGFTGKFA
                     GVVEVLAPQGVDFDRLVAIGTGKAAALDEYAWLKLGGTIAASLRMAADATVVLDAPDV
                     SGNAEEAAALAAGILLRSYRFDKYKTRKDEAEGEGASKKPAKITIQCADPGKAKKA"
     gene            358..672
                     /locus_tag="VGM46_10680"
     CDS             358..672
                     /locus_tag="VGM46_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193097.1"
                     /translation="MNPATGNALSSVPAGSHAAATSLNLAFSPDSTKTAPFEGTANLA
                     KAMEGRDFMILDFQGGNDALTFGRFAAFSKAPPKNMRDLTVFQQGYLSHVAARRLGVT
                     IC"
     gene            829..2007
                     /gene="lptF"
                     /locus_tag="VGM46_10685"
     CDS             829..2007
                     /gene="lptF"
                     /locus_tag="VGM46_10685"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEY4193098.1"
                     /translation="MKVVERYIMRRTFVLFMGTLLWTLAIVWTTQVLARIDLVTDSGQ
                     SALTFFQVASLIIPSIIPVVVPFALVVAVAQTLSTMNADSELVVISAAGSSRLTVVRP
                     ILLLALAAGLLCFVVHNGLDPYARQKNRELVASARADFLSLIIQEGTFRKVEDGLFVQ
                     IGERFPDSRLGGIFVADSREPGVDLIYYAKTGAIIDRGDKKVLLMNDGVVNRKVPSGD
                     VSVVRFTAYAFDLSAFASDTGDAILLPKDQTTQYLLNPDANDPTFKESPGLYRTELNI
                     RFSEWLFPIAFALIALAVAGDPKSHREARIHPLITAIVICLAVRWLAFFAEGESKSTP
                     FLGYMMYAIPILASLISILFIRVQKTMELPMNWVDAGASVFRGIRDRLTGAPSRQGAA
                     "
     gene            2004..3092
                     /gene="lptG"
                     /locus_tag="VGM46_10690"
     CDS             2004..3092
                     /gene="lptG"
                     /locus_tag="VGM46_10690"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006466414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEY4193099.1"
                     /translation="MMGWTLGRYFFFRYAMITLWFFLGIFALIFLIDFTELTGRTARL
                     PGFTYQTGLMISAFRVPMIMLQTVPFIGLFSAMAMLVSLNRKYELVIARSAGISAWQF
                     LLPACLGALLFGVLSVGVLNPVAAYGFSYSERMESQLRSGASNAISANAAPWLRQKTA
                     SGDTVIGAHAILNQGMEMSDASFFILDAEGNIAERKDAKHVYLRDGYWDLRDVKIFRE
                     SRAVEARATDRVATNLKPEFVQERLARPETIPFYELPQKIEIARSFGLKANAFAMQFD
                     SLVALPFLLVAMTLIAATVSMRFARMGQSATMILGGVFAGFLLYVVSVLVKAFGIAGF
                     VPTVVAAWFPVVVSMFFGVTFLLYKEDG"
     gene            3131..5506
                     /locus_tag="VGM46_10695"
     CDS             3131..5506
                     /locus_tag="VGM46_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019170538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HEY4193100.1"
                     /translation="MAHLYGATALACLFSFAFPATSAFAQDVSGLAPKVAAGSQMLLA
                     ADTLVYDNDAKTVTAVGGVQIDYDGNRLVAQRVTYDRNTKRLLASGNVQLVNSDGTKL
                     YSDHLDITDDFGNGFINALRVETPDKTYMAAESAERMGGVLTTFHNGVYTACEPCEDK
                     PDKAPIWRVKARKVIWNGEKKTVRFEDSNFEFFGFPMAYLPAFEIADPTVKRKSGFLM
                     PGISYKTELGVGVTVPYYLALSPTYDLTAKGTYYSKQGFLGEAEWRQRFDNGQYSVKF
                     AGIQQRDPSAFDPGTVDSGPAGDPKKFRGMMGTKGQFAINSRWNFGWDILTQTDKNFS
                     YTYNVQDYNSYVHRSEVFLTGLNDRNYFDLRAMHFEVQEEALDGVNGSRSKQQPWVLP
                     SLDYAYIPETPLAGGQLTFNLNTRVIHRDDLDEAFNTPYNALTPTQRVRGIHGDSGRI
                     TGEAEWKRTFITDGGLVITPLLAFQADADYVNASSDSIAAINSMATNPMIGSTADDRS
                     SFARFMPTAGLELRYPILFSMGHSSHVLEPMVQTFVRPNEQYVGGLGIPNEDAQSFVF
                     DATTLFERDKFSGYDRVEGGTRANVGVRYSGSYENGWTTNALFGQSYQIGGRNSFATP
                     DLVNVGAYSGLETAKSDFVALAGLASPSGLSASVSGRFDENTLEARRTELRAGYSGAP
                     LSLTSSYTYIQAQPLYGFETDRHEVSLGASTKLGRFWSVFGSGTYNLEDSYMASKGVG
                     FSYADECFTYLMTFSETQSSKHDEPSQNIGFNISFRTLGDFGAASNSFAQQ"
     gene            5599..6525
                     /locus_tag="VGM46_10700"
     CDS             5599..6525
                     /locus_tag="VGM46_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY4193101.1"
                     /translation="MRKYLFSAGFALLVMATAISLAATIPSAFASAIKYIVNDVPITS
                     GDIQHRAAFLRLQHKKADAGDELIDQTLRLAEAKRLGIRISDAQVNEAYARFASSNKM
                     QLKQLDTVMARSGVTKEHFKEFIRAQMAWNQALGARFRAESSGSGNSEQDMVRRMLEK
                     GGDKPKATEYMLQQVIFVVPAAERKSAMGKRKREAEAMRDRFNGCENTRQFAKGLIDV
                     TVRELGRVLAPQLPPEWAEQIKATKAGGATTVRETERGIEFIGVCSAREVSDDKVAQM
                     VFDTEGKTDDKADALSAKYIGELKAKARIVRR"
     gene            6533..>7538
                     /gene="pdxA"
                     /locus_tag="VGM46_10705"
     CDS             6533..>7538
                     /gene="pdxA"
                     /locus_tag="VGM46_10705"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEY4193102.1"
                     /translation="MNSSPNASLALSVGDPSGVGPETAITAWSRRQADGVPAFYLLAD
                     PALIETRARFIGLDVKIQPTTPDQASALFGEALPVVPLAARFIDSPGRPDPDNAAGIV
                     EAIDRAVADCLAGDAAAVVTCPIAKKPLYDAGFRFPGHTEYLAHLASRHTGREIMPVM
                     MLSGPDLRTVPVTIHIPLAEVPSALTSELIVATARITAVDLAQRFGIKKPRLAISGLN
                     PHAGEGGAMGDEDERIVRPAIDKLRAEGIDAFGPLPADTMFHPRARAGYDVAICMYHD
                     QALIPAKALAFDEAVNVTLGLPFIRTSPDHGTAFDIAGKGIARPDSLIAALKLARRLA
                     D"
BASE COUNT         1404 a         2453 c         2150 g         1531 t
ORIGIN      
        1 aaggccttct tcgccttgcc gggatcggcg cattggatgg ttattttggc gggcttcttc
       61 gaagcgccct cgccctccgc ctcatccttg cgggtcttgt atttgtcgaa gcggtagctg
      121 cgcagcaaaa tgccggcggc aagggctgcc gcctcttccg cattaccgga aacatccggc
      181 gcgtccagca cgaccgtcgc gtccgccgcc atgcgcaacg aggcggcgat ggtgccgcca
      241 agcttcagcc acgcatattc atcgagcgcg gccgccttgc ctgtcccgat tgccaccagc
      301 ctgtcgaagt ccacgccctg tggcgccagc acctcgacga caccggcgaa cttgcccgtg
      361 aaccccgcca ccggaaatgc tctttccagc gttcccgcgg gatcgcacgc cgcggccacc
      421 tcgctcaatc ttgcattttc gcccgacagt acgaagaccg cacctttcga aggcacagcg
      481 aatttggcaa aggcgatgga agggcgcgat ttcatgattc ttgactttca aggcgggaac
      541 gacgcgctga catttgggcg ttttgcggct tttagcaagg ctccgccgaa aaacatgagg
      601 gatttaaccg tcttccagca gggctatttg tcccatgtcg cggcgcgccg tttaggcgta
      661 accatctgtt aaccatctgt ctttgccctt tcttatccaa tcttgccgac actgagcgcc
      721 aaccggggca acgatgaacc cagagcaatc cggttgtgcg cgcgtcgccg gacagatact
      781 ttgcccggag gcatgatgcc ggtcgaaagc agccgagaaa cagtctgaat gaaggtcgtc
      841 gaacgctaca tcatgcggcg cacttttgtg ctcttcatgg gaacgctgct gtggacgctg
      901 gcgatcgtgt ggacgacgca ggtgctcgca cgcatcgacc ttgtcaccga cagcggccaa
      961 tccgccttga cgttctttca ggtcgcctcg ctcatcatcc cctcgatcat ccccgtcgtg
     1021 gtgccgttcg cgctggtcgt cgcggtcgca cagacgctga gcaccatgaa cgccgattcc
     1081 gagctggtgg tcatcagcgc ggcaggcagt tcccggctga ccgtggttcg gccgatcctg
     1141 ctcctggcat tggcggcggg gctgctctgc ttcgtcgtcc acaacggcct cgacccctat
     1201 gcgcggcaga agaaccgcga actcgtggcc tccgcgcgcg ccgatttcct gtcgctgatc
     1261 attcaggaag gcaccttccg caaggtggag gacggcttgt tcgtgcagat cggcgagcgc
     1321 tttcccgata gccggctcgg cggcatcttt gtcgccgatt cccgcgagcc tggcgtcgat
     1381 ctcatctact acgccaagac cggcgcgatc atcgaccgcg gcgacaagaa agtgctgctg
     1441 atgaacgacg gcgtcgtcaa ccgcaaggtt ccctcgggcg acgtttcggt ggtgcgcttc
     1501 acggcctatg cgttcgacct ttcggccttc gcctcggaca cgggcgacgc catccttttg
     1561 ccgaaggatc agacgacgca atatcttctc aatcccgatg cgaacgatcc gacattcaag
     1621 gaatcgcccg gcctctatcg aaccgaactc aacattcggt tctccgaatg gctgtttccc
     1681 attgcattcg ccttgatcgc cctggctgtt gccggcgatc ccaaatcgca ccgggaggcg
     1741 cggatccacc cgctgatcac ggccatcgtc atctgccttg ccgtgcgctg gctggccttt
     1801 ttcgccgagg gcgaatccaa gagcacgccc ttcctcggct acatgatgta tgcgatcccg
     1861 atcctggcat ccctgatctc catcctgttc atacgcgtcc agaaaacgat ggaactgccg
     1921 atgaactggg tcgacgcggg cgcttccgtc tttcgtggca tccgtgaccg ccttacgggc
     1981 gccccgtcca gacagggagc cgcatgatgg gctggacgct gggccgctac ttcttctttc
     2041 gctatgcgat gatcacgctg tggttcttcc ttggcatatt cgcgctcatt ttcctgatcg
     2101 acttcacgga actgaccggc cgcaccgcgc gtctgcccgg ctttacctat cagacgggcc
     2161 tgatgatttc ggcgttccgg gtcccgatga tcatgttgca gaccgttcct ttcatcggcc
     2221 ttttctccgc catggcgatg ctggtgtcgc tgaaccgcaa atatgaactc gtcatcgccc
     2281 gctccgccgg catatcggcc tggcaatttc tcctgcccgc ctgccttggc gcgctgttgt
     2341 tcggcgtttt gtcggtcgga gtgctcaacc cggtggcggc ctacgggttc tcctattccg
     2401 aacgcatgga aagccagctc cgctccggcg cttcaaacgc catctcggcc aatgccgccc
     2461 cctggctcag gcagaagacc gcttccggcg acaccgtcat cggcgcgcac gccattttga
     2521 accagggcat ggaaatgtcc gacgcctcgt tcttcatcct cgatgccgag gggaatatcg
     2581 ccgagcgcaa ggacgcaaag catgtctacc tgcgcgacgg ctattgggat ttgcgtgacg
     2641 ttaagatttt tcgtgaaagc cgcgccgtcg aagcgagggc gaccgaccgc gtagccacca
     2701 atctcaagcc ggaattcgtc caggaaagac tggcccggcc cgaaaccatc ccgttctacg
     2761 aactgccgca aaaaatcgaa attgcccgct ccttcggact taaggcaaat gccttcgcca
     2821 tgcagttcga ttcgttggtg gcgctgccgt tcctgctggt cgccatgacg ctgattgcgg
     2881 cgacggtatc gatgcgattt gccagaatgg gtcagtctgc aacgatgatt ctgggtggtg
     2941 tcttcgcagg ctttctgctt tatgtcgtct cggtgctggt gaaggcgttt gggatcgccg
     3001 gattcgtgcc gactgtcgta gccgcatggt ttcctgtcgt cgtgtcgatg ttttttgggg
     3061 taacgtttct gctgtacaag gaagacggct agtgaagggg gcagttttgc gtagccgtag
     3121 gacagcccgg atggcgcacc tttacggggc gaccgccttg gcatgccttt tttccttcgc
     3181 gttcccggcc acatccgcat ttgctcagga cgtttccggc ctggcgccca aagtggcggc
     3241 cggctcgcaa atgctgctgg cagccgatac gctggtttat gacaatgatg ccaagacggt
     3301 aaccgccgtc ggcggcgtcc agatcgacta tgacggcaat cgcctggtcg cccagcgcgt
     3361 cacctacgac cgcaacacca agcgcctgct ggccagcggc aatgtccagt tggtcaacag
     3421 cgacggcacc aagctttatt ccgaccacct cgatattacc gacgatttcg gcaatggctt
     3481 catcaatgcg ctgcgcgtcg aaacgcccga caagacctac atggccgccg aaagcgccga
     3541 gcgcatgggc ggcgtgctga cgaccttcca caatggcgtc tacaccgcct gcgaaccttg
     3601 cgaggacaag ccggacaaag cgccgatctg gcgcgtcaag gcgcgcaagg tcatctggaa
     3661 cggcgaaaag aaaacggttc gcttcgagga ttcgaatttc gaattcttcg gctttccaat
     3721 ggcctatttg ccggcattcg agattgccga cccgaccgtc aagcgcaaga gcggcttcct
     3781 gatgcccggc atcagctaca agaccgaact gggcgtcggc gtaaccgttc cctattatct
     3841 tgcgctgtcg cccacctacg acctgaccgc caagggcacc tattattcca agcagggctt
     3901 tctcggcgag gccgaatggc gtcaacgctt cgacaacggc cagtacagcg tcaagttcgc
     3961 cggcatccag cagcgcgatc cgagcgcctt cgaccccggc accgtcgatt ccggtcccgc
     4021 cggcgatccc aagaaattcc gcggcatgat gggcaccaaa ggccagttcg ccatcaattc
     4081 gcgctggaat ttcggctggg atatcctgac ccagaccgac aagaactttt cctacaccta
     4141 caatgtccag gactataata gttacgttca tcgctccgaa gtctttttga ccggattgaa
     4201 cgaccgcaac tatttcgacc tgcgcgccat gcatttcgaa gttcaggaag aagcgctcga
     4261 tggggtcaac ggctctcgtt ccaagcagca gccatgggta ctgcccagcc tggactatgc
     4321 ctacatccct gaaacgccgc tggccggcgg ccagctgact ttcaatctca atacgcgggt
     4381 tatacaccgc gacgacctcg atgaagcctt caacacgccc tataacgccc tgacgccgac
     4441 ccagcgcgtg cgcggcatcc atggagatag cggccgcatc accggcgagg ccgagtggaa
     4501 acgaaccttc atcaccgatg gtgggctggt catcacgccg ctgttggcct tccaggccga
     4561 tgccgattac gtcaacgcct cttccgattc gattgccgcc atcaattcca tggcgaccaa
     4621 ccccatgatc ggctcgacgg ccgacgaccg ctcttccttc gcccgcttca tgcccaccgc
     4681 cgggctggaa ctgcgctacc cgatcctgtt ttcgatgggc cattcgagcc atgttctgga
     4741 gccgatggtc cagacattcg tgcgccccaa cgagcaatat gtcggcggcc tgggtatccc
     4801 caacgaagac gcgcaaagct tcgttttcga tgcgaccacc ctgttcgagc gcgacaagtt
     4861 ctccggctac gaccgtgtcg agggcggcac ccgcgccaat gtcggcgtgc gctattcggg
     4921 cagctacgaa aatggctgga cgaccaacgc gctgtttggc caatcctacc agatcggcgg
     4981 gcgaaattcc ttcgccacgc cggatttggt caatgttgga gcctattccg gcctcgaaac
     5041 cgccaagtcg gacttcgtcg cgcttgccgg cctggccagc ccatccggcc tttcggcatc
     5101 ggtcagcggc cgtttcgatg aaaatacctt ggaggcgcgc cgcacggagc ttcgcgcggg
     5161 ctatagcggc gcgccgttgt cgctcacctc cagctacacc tatatccagg cgcagcccct
     5221 ttatggcttc gaaacggacc gccacgaagt ttcgctcggt gcaagcacca agttgggccg
     5281 gttctggagc gtattcggct cgggcaccta caatctggaa gatagctaca tggcgagcaa
     5341 aggcgtcggc ttttcctatg ccgacgaatg cttcacctat ctgatgacgt tctcggaaac
     5401 gcagtccagc aagcatgacg agccgtcgca gaatatcggc ttcaatattt cattccgtac
     5461 ccttggcgac ttcggtgcgg cgtccaacag ctttgcccag cagtaatgcc gaggccggcg
     5521 acattgtttt gccgtataac gctggcatag gcatcggcgt tggagaggcc cgttttcggt
     5581 agaattggaa cgctttcgat gaggaaatat ctgttttccg ccggatttgc gctgttggtg
     5641 atggcaacgg cgatatcgct ggctgcgacg atcccttcgg cctttgccag cgcgataaag
     5701 tatatcgtca acgatgtgcc gattacctcc ggcgatatcc agcatcgcgc cgccttcctg
     5761 cgtctccagc acaagaaggc cgatgccggc gacgaattga tcgaccagac cttgcgtctt
     5821 gccgaagcca agcggcttgg cattcgcatc agcgacgcgc aggtcaacga ggcctatgcc
     5881 cgctttgcct cgtccaacaa gatgcaactc aagcagcttg acaccgtgat ggcccggtcc
     5941 ggcgtgacca aagagcattt caaggaattc atccgcgccc agatggcttg gaatcaggcg
     6001 ctgggcgcgc gtttccgcgc cgagagcagc ggcagcggta acagcgaaca ggacatggtt
     6061 cggcggatgc tggaaaaggg cggcgacaag cccaaggcaa ccgaatacat gctccagcag
     6121 gtcatcttcg tcgtccccgc tgccgagcgc aaatccgcga tgggcaagcg caagcgggaa
     6181 gccgaggcga tgcgtgaccg cttcaacggc tgcgagaaca cgcgccagtt cgccaagggc
     6241 ctgatcgacg tcaccgtccg cgaacttggc cgggtgctgg cgccgcaact gccgccggaa
     6301 tgggccgagc agatcaaggc gaccaaggca ggcggcgcaa cgaccgtacg ggaaaccgaa
     6361 cgcggcatcg agttcatcgg cgtctgttcg gcgcgcgaag tatccgacga caaggtggcc
     6421 cagatggtgt tcgacaccga aggtaagacg gatgacaagg ccgatgcgct tagcgccaaa
     6481 tatattggcg agttgaaggc aaaagcccgc atcgtccggc gctgaaccag ccgtgaattc
     6541 ttctccaaac gcatcgctgg cgctcagcgt cggcgatcct tcgggcgtcg gcccggagac
     6601 ggcgatcacc gcatggtcgc gccggcaggc cgatggcgtt cccgctttct acctgcttgc
     6661 cgatccggcc ttgatcgaaa cgcgcgcccg cttcatcggc ctcgacgtga aaatccagcc
     6721 gacgaccccc gaccaggcct cggccttatt tggtgaggcc ctgccggtcg ttccgctcgc
     6781 cgcccgcttc atcgacagcc ccggccgacc cgatcccgac aatgcggccg gcattgtcga
     6841 ggcgatcgac cgcgccgttg ccgattgcct tgccggcgat gcggcggccg tcgtcacctg
     6901 ccctatcgcc aagaaaccgc tttacgatgc cggcttccgc tttcccggcc ataccgaata
     6961 tctggcgcac ctggcctcgc ggcatacagg ccgcgagatc atgccggtga tgatgctgtc
     7021 cggaccagat ctgcgcaccg tgcccgtcac catccacatc ccgctggctg aggtgccgtc
     7081 ggcgctgacc agcgaattga tcgtcgccac cgcgcgcatt acagccgtcg acctcgcgca
     7141 acgcttcggc attaaaaagc cgcgcctcgc aatctccggc ctcaaccccc atgcgggtga
     7201 aggcggcgct atgggcgacg aggacgagcg catcgtgcgc cctgccatcg acaagctgcg
     7261 agccgaaggc attgacgcgt tcgggccgct gcccgccgac accatgtttc acccgcgcgc
     7321 ccgcgccggc tatgatgtgg cgatctgcat gtaccacgac caggcgctta tccccgccaa
     7381 ggcgttggcc ttcgacgagg cggtgaacgt cacgcttggc ctgcccttta tccgcacctc
     7441 gccggaccac ggcaccgcct tcgacattgc cggcaagggc atcgcccgcc ctgacagtct
     7501 gatcgcggcg ctgaagctcg cccgccgcct tgccgata
//
LOCUS       DASYCS010000342         2321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_966814,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000342 DASYCS010000000
VERSION     DASYCS010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_966814"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="VGM46_10710"
     CDS             complement(<1..483)
                     /locus_tag="VGM46_10710"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006201285.1"
                     /note="catalyzes the formation of
                     (R)-4'-phosphopantothenate in coenzyme A biosynthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEY4193103.1"
                     /translation="MDQLAPTEKYSPYLFFSAEQWAKFRADTPLTLTEEEIHRLRSLN
                     DPVDLEEVKRIYLSMSRLLSAHVEASQLLFRQRRAFFNVGDVVKTPFIIGIAGSVAVG
                     KSTTARVLKELLARWPSSPKVDLVTTDGFLLPNEILRRENLMERKGFPDSYDVGALLR
                     F"
     gene            complement(507..833)
                     /locus_tag="VGM46_10715"
     CDS             complement(507..833)
                     /locus_tag="VGM46_10715"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006325660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEY4193104.1"
                     /translation="MAAFSLFDLEKNIHDRAHSGDPDSWTARLYARGIEKAAQKLGEE
                     AVETVIAAVTGDNKALVSESADLLYHWLVVLGISGVPLDAVLDELAQRAGRSGIAEKA
                     SRQNPG"
     gene            complement(842..1636)
                     /gene="hisF"
                     /locus_tag="VGM46_10720"
     CDS             complement(842..1636)
                     /gene="hisF"
                     /locus_tag="VGM46_10720"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015314587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEY4193105.1"
                     /translation="MTLKARVIPCLDVKDGRVVKGVNFVDLIDAGDPVEAAKAYDAAG
                     ADELCFLDITASSENRETIFDIVARTAEQCFMPLTVGGGVRQVADIRRLLLAGADKVS
                     INTAAVKNPDFVAEAAQKFGNQCIVVAIDAKKVSMPGQADRWEIFTHGGREKTGIDAV
                     EFARKMVDLGAGEILLTSMDRDGTKAGYDIALTRAVADAVRVPVIASGGVGSLDHMVD
                     GISGGHATAVLAASIFHFGTYSIAEAKAHMAKAGLPMRLDTAAFRS"
     gene            complement(1847..>2321)
                     /locus_tag="VGM46_10725"
     CDS             complement(1847..>2321)
                     /locus_tag="VGM46_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019170911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HEY4193106.1"
                     /translation="ARVILGTVAVRDPELVKQACKAFPGKVAVGIDAKGGKVAVEGWA
                     EASSLGVVELARTFEGAGVAAIIYTDIDRDGVLAGINWESTIALAEAVSIPVIASGGL
                     ASIADIVRMTMPDARKLEGAISGRALYDGRIDPAQALAILRGEGNPPAALFEERP"
BASE COUNT          419 a          740 c          737 g          425 t
ORIGIN      
        1 gaagcgcagc agcgcgccga catcgtagct gtcgggaaag cccttgcgct ccatcaggtt
       61 ttcccggcgc aggatttcgt tgggcaacag gaagccgtcg gtggtgacga gatcgacctt
      121 gggactggac ggccagcgcg ccagcagttc tttcagcacg cgcgccgtgg tcgatttgcc
      181 gacggcgacc gaaccggcaa tgccgatgat gaacggcgtc ttgaccacgt cgccgacatt
      241 gaagaaagcg cggcgctgac gaaacaaaag ctggcttgcc tcgacatgcg ccgacaaaag
      301 ccgcgacatc gacagataga tgcgcttgac ctcttccagg tcgaccgggt cgttcagtga
      361 acgcaggcgg tggatttctt cttcggtcag cgtcagcggc gtatcggcgc gaaattttgc
      421 ccattgctcg gccgagaaga agagataggg cgagtatttc tcggtggggg cgagttggtc
      481 catcgcgtct tctgccctcg tctggctcag ccgggatttt gccgggaggc cttttcggcg
      541 atgcccgagc ggccggcgcg ctgcgcaagt tcatccagca cggcatcaag cggaacgccc
      601 gaaataccca gcaccaccag ccaatgatag agaagatcgg cgctttccga aaccagcgcc
      661 ttgttgtcgc cggtgacggc cgcgatcacc gtttcgacgg cttcctcgcc cagtttctgc
      721 gccgcctttt cgatgccgcg cgcataaagc ctggccgtcc atgaatccgg atcgccggaa
      781 tgggcacggt cgtgaatgtt tttttcgagg tcaaagagcg aaaacgcggc catggcgccg
      841 tttaagaccg aaaagccgcg gtgtccagcc gcatgggcaa gccggctttc gccatatgcg
      901 ccttggcctc ggcaatcgaa taggtgccga aatggaagat ggaggcggca agcacggctg
      961 ttgcgtggcc gccggagatg ccgtccacca tatggtcgag gctgccgacg ccgcctgaag
     1021 cgatcaccgg cacgcgcacg gcatcggcca ccgcgcgggt cagcgcgata tcatagccgg
     1081 ccttggtgcc gtcgcgatcc atcgaagtga gcaggatttc gccggctccc aggtcgacca
     1141 ttttccgcgc gaactcgacc gcgtcgatgc cggtcttttc gcgcccacca tgggtgaaga
     1201 tttcccagcg gtccgcctgg cccggcatcg ataccttctt ggcgtcgatc gcgacgacga
     1261 tgcattgatt gccgaatttc tgcgccgcct cggcgacgaa atcggggttt ttcaccgccg
     1321 ccgtattgat cgacaccttg tcggccccgg ccagcaaaag cctgcggata tcggcgacct
     1381 ggcggacgcc gccgccgacc gtcagcggca tgaagcactg ttcggcggtg cgcgcgacga
     1441 tatcgaagat agtctcgcgg ttttccgaag aagcggtaat gtctaggaag caaagctcat
     1501 ccgcgcccgc cgcgtcatag gctttcgccg cctcgaccgg atcgccggca tcgatcagat
     1561 cgacgaagtt gacgcccttg acgacgcggc cgtcctttac gtcgagacac gggatgacgc
     1621 gagccttgag ggtcaaagga cggcctccat tttcgcctgc gcgtcctcgc cgctacgcgg
     1681 ctgcgggcgg cgcgggcagt cctttacgtc caggcaagga ataacgcggg ccttaagggt
     1741 caaaggacgg cctccatttt cgcctgcgcg tcctcgccgc tgcgcggctg cgggcggcgc
     1801 gggcagtcct ttacgtcgag acacgggata acgcgggctt tcagcgtcat ggccgttcct
     1861 caaaaagtgc cgcgggtgga ttgccttcgc cacgcaatat cgccagcgcc tgtgccggat
     1921 cgatgcgacc gtcatagagc gcgcggcccg agatcgcgcc ttccagcttg cgcgcgtcgg
     1981 gcatggtcat gcgcacgata tcggcgatgg aggcgaggcc gcccgatgct atgaccggga
     2041 tggacaccgc ctcggccagc gcgatggtcg attcccagtt gatgccggca agcacgccgt
     2101 cgcggtcgat atcggtgtag atgatggcgg caacgcccgc accttcgaat gtccgggcca
     2161 gttccactac gccaagcgaa gaagcttccg cccagccctc gaccgccacc ttgccgccct
     2221 tggcgtcgat gccgacggcg accttgccgg ggaacgcctt gcaggcctgc ttcaccagtt
     2281 cgggatcgcg caccgccacc gtgccgagga tgacgcgggc a
//
LOCUS       DASYCS010000343         9794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_239460,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000343 DASYCS010000000
VERSION     DASYCS010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 9794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9794
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239460"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="VGM46_10730"
     CDS             complement(<1..567)
                     /locus_tag="VGM46_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEY4193107.1"
                     /translation="MMVGVRHDDLRRRNRAMVISAVRRAAGEASRTAVAAETGLSHST
                     ISAISSDLIAEGILNESRDAPPSLKRGRPQVGLALNARATSVLSVVLSLNFLSIAVID
                     YAGGVIAQEERRLDTQAMSREQLVAECVAIIRHRLDDPGFAGGKVARIVLAVQGTTDA
                     AARTVLWSPITPQTDIAFAAILEEEFGIP"
     gene            799..1839
                     /gene="xylF"
                     /locus_tag="VGM46_10735"
     CDS             799..1839
                     /gene="xylF"
                     /locus_tag="VGM46_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015340758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_function: GO:0033222 - xylose binding [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY4193108.1"
                     /translation="MRKFTAAILASAALSLTLVSVAQAKDKIVGVSWSNFQEERWKTD
                     EAAIKKALEANGDKYISADAQSSAAKQLTDVESLISQGANALIILAQDASAIGPAVQK
                     ALDEGIPVVGYDRLIENKDVFYLTFDNKEVGRMQAREVFKAKPEGNYVFIKGSGADPN
                     ADFLFAGSMEVLKEAIDGGKIKNVGEAYTDGWLPANAQKNMEQFLTANDNKVDAVIAA
                     NDGTAGGVVAALTAQGLAGTVPVSGQDADHAALNRIALGTQTVSVWKDARDLGKNAAE
                     IASQLADGKQMTDIAGQKDFTTPGGNTVKSLFLTPVAITKDNLDTVIDAGWISKDEVC
                     AGVKAGAVPACK"
     gene            2054..3373
                     /locus_tag="VGM46_10740"
     CDS             2054..3373
                     /locus_tag="VGM46_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY4193109.1"
                     /translation="MAETTTPSATPEGARASELSAIGRFLKATELDTRMLGMVGALLV
                     IWVGIHFLSGGLFLTPRNLWNLSVQSSSVAIMATGMVLVIVTRNIDLSVGSVEGVIGM
                     VMGVAQAMFLIHVVGLQSGNPAIWIIALVCGLVLGLVIGALQGVIIAYLEVPSFIVTL
                     GGLLVWRGMAWMATSGQTVAPLDATFQLMGGGPRGSIGATWSWVVGAVACLAVVFMLL
                     NGRAQRQRFKFPLRPVWAETFLGAVTCAVILGAVWVANSYPWPVGIVRQYAQQNGITL
                     PEGGLFIAHGIAIPVLMAIAVGIVMTFITGRTRFGRYVFAIGGNPEAAELAGINTRWV
                     TVKVFMIMGMLAAISAAISSARLNAATNALGTLDELLVIAAAVIGGTSLAGGSGTIVG
                     AMLGALLMQSLQSGMVLLGIDSPLQSIVVGAVLVIAVWLDTVYKKRV"
     gene            3389..4177
                     /locus_tag="VGM46_10745"
     CDS             3389..4177
                     /locus_tag="VGM46_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006328044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY4193110.1"
                     /translation="MAETQTPLIDMQNISIAFGGIRAVDDASIDLFPGEVVALLGHNG
                     AGKSTLIKILSGAYKRDSGKIFVNGEEASISNPRDAKKYGIETIYQTLALADNVDAAA
                     NLFLGRELMTSWGMLDDGAMEAEARKVMGRLNPRFQRFKEPVVKLSGGQRQSVAIARA
                     ILFNARILIMDEPTAALGPQETAQVGELVKQLKSEGIGIFLISHDIHDVFDLADRLCV
                     MKNGQVVGTARTADVTPDEVLGMIILGKCPPGAIPGPGALKIAA"
     gene            complement(4288..4536)
                     /locus_tag="VGM46_10750"
     CDS             complement(4288..4536)
                     /locus_tag="VGM46_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HEY4193111.1"
                     /translation="MRSMVLALVAVLALSGCTTTEQDVGVGTAAGAVIGGIAGGGKGA
                     LIGAGAGALGGLLVRNLRDGRCQYRNPNTGRIYTRRCN"
     gene            complement(4618..4941)
                     /locus_tag="VGM46_10755"
     CDS             complement(4618..4941)
                     /locus_tag="VGM46_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193112.1"
                     /translation="MEHIAALLLIVGCSNNLTQCRELPVSVSVFETMAECSAERPFAV
                     GDLADPAKRIFSRCLAVDPALEDDYNEIAWNVRPDGTFEASVEISGVMVASDESHAKD
                     GLRRE"
     gene            complement(5007..5177)
                     /locus_tag="VGM46_10760"
     CDS             complement(5007..5177)
                     /locus_tag="VGM46_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193113.1"
                     /translation="MTVADMKRLEHAARLSMGGLLDADMPAKPFWHARSISLAGLALF
                     AAVLAATMVHLI"
     gene            complement(5314..6342)
                     /locus_tag="VGM46_10765"
     CDS             complement(5314..6342)
                     /locus_tag="VGM46_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HWE histidine kinase domain-containing protein"
                     /protein_id="HEY4193114.1"
                     /translation="MSFETGKDKKGAASDEVVALPIEKDAQLGRALLHALRNAGVSVL
                     YQDSDLKTVWARNMRAPWDQGDEMDGPLPDMQAERLESARRKVARTAEPEHFELSLPG
                     STGMRWFDVWVDADLDENGHVLGIMTAKVETTEQKRREQTLKILLREVSHRSKNLLAI
                     IQSIATQTGRYTDNVADFLNRFRGRLHSLASSQDLVTSSNWRGAELHELIFGQLGRYG
                     SNPAQNLRLSGDSPYLNPNAALHIGLAMHELAVNSVSYGALSRPNGFVEVKSELTRSP
                     SGDKVLCLTWLENIGNNDNQRNHKRFGSVALERIVPTSLNGKSSLEITGDRLEYRLTV
                     PHGNFESD"
     gene            complement(6524..6688)
                     /locus_tag="VGM46_10770"
     CDS             complement(6524..6688)
                     /locus_tag="VGM46_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HEY4193115.1"
                     /translation="MLYWTLVFLVVAIVAGALGFGGIAGASAGLAKILFFIFLAFLVI
                     SLIAGMFRRA"
     gene            complement(6772..7566)
                     /locus_tag="VGM46_10775"
     CDS             complement(6772..7566)
                     /locus_tag="VGM46_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY4193116.1"
                     /translation="MSLSATIAPHLPFLRRFSRAVSGSQESGDALVAAMLEAIIADVD
                     IYPQASSDRIALYKIFAKLFTSVAIRVPQEQAQTAWEQRAAANLNALAPLPRQAFLLV
                     AVEGFSESEAAEILGVDDGAFSDLLAQASNEISRQVATDILIIEDEPLIAMDIEEMVE
                     SLGHRIVGMARTHAEAVALFGKTRPRMVLADIQLADGSSGIDAVNEILASTSVPVIFI
                     TAFPERLLTGERPEPAFLVTKPFNPDMVKALISQALFFDRQAKAAA"
     gene            7807..8007
                     /locus_tag="VGM46_10780"
     CDS             7807..8007
                     /locus_tag="VGM46_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NepR family anti-sigma factor"
                     /protein_id="HEY4193117.1"
                     /translation="MKEKTQGPAARNGSRGRLSGDPLGANSEIGRKLRQYYDDLVSDE
                     VPDRFTQLLAQLEQTEPPVKKG"
     gene            8011..8559
                     /locus_tag="VGM46_10785"
     CDS             8011..8559
                     /locus_tag="VGM46_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY4193118.1"
                     /translation="MAVSQGFKTDLLGSIPSLRAFAISLTQSADKADDLVQETLVKAW
                     DKHESFQMGTNLKAWLFTILRNEFYSQMRKRGREVQDSDGIMTARLAVHPSQHGQLDL
                     KDFRGALEQLPEDQREAIILIGASGFSYEEAAKICDCAVGTIKSRVSRARSKLQEILK
                     LSGEEDYGPDAISAQVMGSPTT"
     gene            complement(8532..9008)
                     /locus_tag="VGM46_10790"
     CDS             complement(8532..9008)
                     /locus_tag="VGM46_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY4193119.1"
                     /translation="MSQLLDGLHILVLEDEFLIAMDVEQLCRDYGAREVTILRSLAGA
                     DGAGIGEAGAGVDGAEMDGRGLPIRFDAAIVDLMLDGVSTLDFAKTLFERKVPFIFAS
                     GYCDVAEIEAGFPGVAMITKPYSGIDLIQALAASCGRPVERPMGQPTDQVVGDPIT"
     gene            9203..9529
                     /locus_tag="VGM46_10795"
     CDS             9203..9529
                     /locus_tag="VGM46_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193120.1"
                     /translation="MALAAGKPVNEGVVNSDLEADIAQLKADIEKLTRQLAATGEHGY
                     GAARRAAAEGVENLRVQGEAALDSLRGSAQDIEAQVIASVREKPVTSLAIAAGVGFLF
                     AMLARR"
     gene            9536..>9794
                     /locus_tag="VGM46_10800"
     CDS             9536..>9794
                     /locus_tag="VGM46_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193121.1"
                     /translation="MGMLASLIAGLASGETAAAIRRARMAAMVYLVAGVAALCGIGFL
                     IGAAFLWTSFRYGSIHAALGFGVGFLVLAGIVVLAFKLFSNR"
BASE COUNT         1957 a         2973 c         2979 g         1885 t
ORIGIN      
        1 cggaatgccg aattcctcct ccagtatggc ggcgaaggca atgtcggtct gcggcgtgat
       61 cggcgaccac aatacggtgc gcgccgccgc atcggtggtg ccctggacgg caagcacgat
      121 acgcgccacc ttgcccccgg caaaacccgg atcgtcaaga cggtgtcgga tgatggccac
      181 gcattcggcg acaagctgct cgcgcgacat ggcttgcgtg tcgagccggc gttcctcctg
      241 cgcgataacc ccgcctgcat agtcgatgac ggcgatggaa aggaaattca gcgacaacac
      301 gacgctcaac accgatgtcg cccgcgcgtt cagcgccagc cccacctgcg ggcgtccgcg
      361 cttcagcgag ggcggggcat ccctgctttc gttgaggata ccctcggcga tcaggtcgga
      421 tgaaatggcg gatatggtgg agtggctaag gccggtttca gccgcgaccg cggtgcgcga
      481 tgcttcgccc gccgccctgc gcacggccga aatgaccata gcgcggttgc ggcggcgcag
      541 atcgtcgtgg cgcactccca ccatcatctt gcctgcgtct cccaaggcat caatagtcct
      601 acaattatgg gaagccggcg gcgctgtcat tgattatttc gagaggcgaa aaaatatccg
      661 ggccgaaaat cccggttgac agttcgagac ggttggacga gtattttttc gatgctcgaa
      721 aaaaagagct tcagtgccgg cctgggcaat gttcgggcca acagggtcgc gccgtaggct
      781 gcgcagggag aagaaatcat gaggaagttt actgccgcca ttttggcgag cgccgcgctt
      841 tcgctgacat tggtgtcggt cgcgcaggcg aaggacaaga tcgttggcgt ttcctggtcg
      901 aactttcagg aagagcgctg gaagactgac gaagccgcca tcaagaaggc gctcgaagcc
      961 aatggcgaca agtatatttc ggctgacgcg cagtcttcgg ccgccaagca gctcaccgat
     1021 gtggaaagcc tgatttcgca aggcgccaat gccctgatca tcctcgccca ggatgcttcg
     1081 gcaatcggcc ccgccgtcca gaaggcgctc gacgaaggca ttccggtcgt cggctacgat
     1141 cgcctgatcg aaaacaagga cgtgttctac ctgaccttcg acaacaagga agttggccgc
     1201 atgcaggccc gcgaagtgtt caaggccaag ccggaaggca attatgtctt catcaagggt
     1261 tccggcgccg atccgaatgc cgatttcctg tttgccggtt cgatggaagt gctcaaggaa
     1321 gccatcgacg gcggcaagat caagaatgtc ggcgaagcct ataccgatgg ctggctgccc
     1381 gcgaacgccc agaaaaacat ggaacagttc ctgaccgcca acgacaacaa ggtcgatgcg
     1441 gtgattgcag ccaatgacgg caccgccggc ggcgtcgttg cggcgctgac cgcgcagggc
     1501 cttgccggaa cggtgcctgt ctcgggccag gacgccgacc acgcggcgct gaaccgcatc
     1561 gcgctcggca cgcagacggt gtcggtgtgg aaggacgcgc gcgacctcgg caagaacgcc
     1621 gctgagatcg cttcgcaatt ggccgatggc aagcaaatga ccgatattgc cggccagaag
     1681 gatttcacca cgcccggcgg caacaccgtc aagtcgctgt tcctgacgcc tgtcgccatc
     1741 accaaggaca atctcgacac cgtcatcgac gctggctgga tttccaagga tgaggtctgc
     1801 gccggcgtca aggccggagc tgtccctgcc tgcaaatagg ccagggtctc ttggttgcaa
     1861 atgccgcgcc cacgggcgcg gcatttagcg atttgagctt caatttatcc tgaaacatac
     1921 ctagaattgc agttcgctgg agcggttcgt ttcgacgatt gcggtgaacc gcttcagttc
     1981 tttgttttgc aattccgggt gggcaggttt ttccgtcttc ccggaatagc gtcagccggc
     2041 ggggaagaca ggcatggccg agacgacgac gcccagcgca acgcccgaag gcgcccgcgc
     2101 ttccgaactc agcgccatcg gccggtttct gaaggcgacc gaactcgata cgcgcatgct
     2161 gggcatggtc ggcgcgctgc tggtcatctg ggtcggcatc cattttctgt ccggcggcct
     2221 gttcctgacg ccgcgaaacc tatggaattt gtcggtccag agctcgtccg tggcgatcat
     2281 ggccaccggc atggtgctgg tcatcgtgac gcgcaacatc gacctttccg tgggttcggt
     2341 cgagggcgtg atcggcatgg taatgggcgt ggcgcaggcc atgttcctga tccatgtggt
     2401 cggcctgcaa tcgggcaatc cggcgatctg gatcatcgcg ctggtctgcg gcctcgtcct
     2461 cggcctggtg atcggcgcgc tacagggcgt catcatcgcc tatcttgagg ttccctcctt
     2521 catcgtcacg ctgggcgggc tgctggtctg gcgcggcatg gcctggatgg ccaccagtgg
     2581 ccagacggtg gcgccgctcg atgcgacctt ccagctgatg ggcggcggcc cgcgcggctc
     2641 catcggcgcg acatggagct gggtggtagg cgcagtcgcc tgcctggcgg tcgtgttcat
     2701 gctgctcaac ggcagggcgc agcgccagcg tttcaagttc ccgctgcggc cggtatgggc
     2761 ggagacattc ctgggcgcgg tcacctgcgc cgtcattctc ggcgcggtgt gggtcgccaa
     2821 ttcctatccc tggccggtcg gcatcgtgcg ccaatacgcc cagcaaaacg gcatcaccct
     2881 tcccgaaggc ggcctgttca tcgcccatgg catcgcaatc cccgtcctga tggcgattgc
     2941 ggtcggcatc gtcatgacct tcatcaccgg ccgcacccgt ttcggacgct atgtgttcgc
     3001 catcggcggc aacccggaag cagccgagct tgccggcatc aatacgcgct gggtgacggt
     3061 caaggtgttc atgatcatgg gcatgctggc ggcgatcagc gccgccattt cctccgcccg
     3121 cctcaacgcc gcgaccaacg cgctcggcac gctggacgaa ttgctggtga tcgcggcggc
     3181 cgtcatcggc ggcacatcgc tggccggcgg ctccggtacg attgtcgggg ccatgcttgg
     3241 cgcgctgctg atgcagtcgt tgcaatcggg catggtgctg ctcggcatcg attcgccgct
     3301 acagagcatc gtcgtcggcg ccgtgctggt catcgccgtg tggctcgata ccgtctacaa
     3361 gaagcgggtt tagggaagga gcaggatcat ggcagaaacc cagaccccgc tgatcgacat
     3421 gcaaaacatc tcgatcgcct ttggcggcat tcgcgccgtc gacgacgcgt cgatcgacct
     3481 gttccccggc gaagtggtgg cgctgctcgg ccataatggc gcgggcaagt cgacgctgat
     3541 aaaaatcctg tccggcgcct acaagcgcga tagcggcaag attttcgtca atggcgagga
     3601 ggcctcgatt tccaacccgc gcgacgccaa gaaatacggc atcgagacga tctaccagac
     3661 gcttgccttg gccgacaatg tcgatgccgc cgccaacctt ttccttggcc gcgaactgat
     3721 gacaagctgg ggcatgctgg atgacggtgc gatggaagcc gaagcgcgca aggtgatggg
     3781 ccggctcaac ccgcgcttcc agcgcttcaa ggaacccgtc gtcaagctct ccggcggcca
     3841 gcggcagtcg gtggccatcg cgcgcgccat cctgttcaac gcacgcatcc tcatcatgga
     3901 cgagccgacg gcagcgcttg ggccacagga gacggcgcag gtcggcgaac tggtcaagca
     3961 attgaagtcg gaaggcatcg gcatcttcct catcagccac gacatccatg acgtattcga
     4021 tcttgccgac cgcctctgcg tcatgaagaa cggacaggtg gtcggaaccg cgcgcaccgc
     4081 cgacgtgacc cccgatgagg tgcttggcat gatcatcctc ggcaaatgcc cgcccggcgc
     4141 catccccggt cccggcgcgc tgaaaatagc cgcctgatcg cctacggcaa tcagcccctc
     4201 tcagtttcgg ccagccttcg atgcggcggc atttgaaaat gaaaaaggcc ggcatggagc
     4261 cggccttttt cattcatgtc gctcgcgtca gttgcagcgg cgcgtataga tgcggcccgt
     4321 gttgggattg cggtactggc agcggccatc gcgaagattg cgcaccagaa ggccgcccag
     4381 cgcaccggcg ccagcgccga tcagcgcgcc tttgccgccg ccggcgatgc cgccgatgac
     4441 agcacctgcc gccgtgccca caccaacgtc ctgctcggtc gtcgtgcaac cgctgagcgc
     4501 caataccgcg accagggcaa gaaccatgct tctcatcaat tcactcctct cagttccatt
     4561 ttcatttgtc gtcccggaac tcttatagcc gaggccgggt aacctgccta gaatgcttca
     4621 ctcgcgacga agcccatctt ttgcatggct ttcatccgac gcgaccatca cgcctgaaat
     4681 ctccacggaa gcttcgaacg tgccatcggg gcgaacgttc catgcgatct cgttgtaatc
     4741 gtcctcaagg gccgggtcga ccgcgaggca gcggctgaaa atgcgctttg caggatcagc
     4801 gaggtctccg accgcaaatg gccgttcagc gctgcattcg gccatcgtct cgaatacgct
     4861 gacggaaacg ggcagctcgc ggcattgagt gaggttgttc gagcaaccga cgatcaaaag
     4921 cagagcagcg atgtgttcca tggcgatttc ctctcgccgg atgaaacggc caagacccgc
     4981 caaagttcct gatcgctctt aagcggtcag atcaggtgaa ccatcgtcgc tgccaacacc
     5041 gccgcaaaca gggccagtcc ggccaaggaa atggagcgcg catgccagaa cggtttggca
     5101 ggcatatcgg catcgagaag accgcccatc gaaagacggg ccgcatgttc caatcgcttc
     5161 atgtcggcaa cggtcaaaat ggtctccata aattgtcggg aacacagccg gaaacgtttt
     5221 cgcccatgcc cggttccagg aatctgcaac gataagatcg ggttttatgg taaacgcgcg
     5281 gttaacgatg caagcttcac gctggccggt tgctcagtcg gactcgaagt ttccgtgtgg
     5341 cacggtgagc cggtattcca gccggtctcc ggtgatctcc agcgacgatt ttccattcag
     5401 cgatgtcggc acgatacgct ccagcgccac gctgccaaag cgcttatggt tgcgctggtt
     5461 gtcgttgttg ccgatatttt cgagccaggt caggcagagc accttgtcgc cggacggtga
     5521 cctggtcagc tcggacttga cctcgacgaa gccgttaggc cgtgagagcg cgccgtaact
     5581 taccgaattg acggccagtt catgcatggc caagccgata tggagggccg cattgggatt
     5641 gagatagggg ctgtcgcctg aaagccgcag gttttgcgcc gggttggagc cataacgccc
     5701 gagctgcccg aagatcagtt catgcagctc cgcgccgcgc cagttggaag aggtcaccag
     5761 atcctgggag gaggccagcg agtgcagtcg cccgcgaaac cggttgagga agtcggcgac
     5821 attatcggtg tagcgaccgg tctgcgtcgc tatgctttgt atgatggcga gcaggttctt
     5881 cgaacgatgg ctcacctccc tcagcaatat tttcagcgtc tgttcccggc gcttttgctc
     5941 ggtggtttcg actttggcgg tcataatccc caggacatgg ccgttctcat cgagatccgc
     6001 atcgacccag acatcgaacc agcgcatgcc cgtcgagccg ggcaggctga gttcaaaatg
     6061 ctccggctcg gctgttcggg caaccttgcg ccgcgccgat tcgagacgct cggcctgcat
     6121 gtcaggcagc ggcccatcca tctcgtcgcc ttggtcccat ggcgcgcgca tgttgcgcgc
     6181 ccataccgtt ttcaagtcgc tgtcctgata gagaaccgaa acgccggcat tacgcagggc
     6241 atggagaaga gcgcgcccaa gctgggcgtc tttctcgata ggcagggcaa cgacctcgtc
     6301 gctggcggcc ccttttttat ctttgcctgt ctcaaagctc atttgccgat caatccactc
     6361 gataccagga agcaggctcg aacggaggta caacctttca gttcgcatta ggttcaaaaa
     6421 cagttccgcc gggccgcgcc cttgacgggc gctacccggc ggcagctgga aaccgtttga
     6481 ggggtgcggc ttccagtgtc taggaatgct ttgaaggaac gcgtcacgcc cgcctgaaca
     6541 tgccggcgat aagcgaaatc accaggaagg cgaggaatat gaagaagaga atcttcgcca
     6601 atcccgccga tgcgcccgca atgccgccaa agcccaaggc tccagcgacg attgccacca
     6661 ccaggaatac cagcgtccag taaagcatga ccaactcctt aggggtctgt ttgaaaacgc
     6721 gacgttttac cgttggttcc gctcttcaaa aaagaagagc gttgccggtc gtcaggccgc
     6781 agcctttgcc tgcctgtcga agaacaatgc ctggctgata agcgccttga ccatatcggg
     6841 attgaacggc ttggtgacca ggaaggcggg ctcggggcgt tcgccggtca gcaggcgctc
     6901 cggaaaggca gtgatgaaga tcaccggcac cgaagtcgag gccagtatct cattgacggc
     6961 gtcaatgccc gaactgccat cggcaagctg gatgtcggcc agcaccattc ttggccgcgt
     7021 cttgccgaaa agcgctaccg cctcggcatg ggtacgcgcc atgccgacga ttcggtggcc
     7081 aaggctttcg accatttcct cgatgtccat cgcaatcagc ggctcgtcct cgatgatcag
     7141 tatgtcggtc gccacctgac gcgagatttc attgcttgcc tgggcaagca gatcggaaaa
     7201 cgcgccatcg tcgacgccga ggatttcggc cgcctcactt tcgctgaacc cttcaacggc
     7261 gaccagcagg aaggcctgcc gcggcagcgg cgccagcgca ttcaaattgg cggcggcgcg
     7321 ttgttcccag gccgtctggg cctgctcctg cggcacccgg atggccaccg aggtaaaaag
     7381 cttggcaaat atcttgtaaa gcgcaatgcg gtcgctggaa gcctgcggat agatatcgac
     7441 atcggcaatg attgcctcca gcatcgccgc cacgagagcg tcgccgcttt cctgtgaccc
     7501 cgacacggcg cgcgaaaagc ggcgcaggaa cggcagatgc ggagcaattg ttgcggatag
     7561 actcatggtg accatctccc tcagatgacg tatttgctta aaatgcaggc tggatgaatt
     7621 gcaagcccgg tgtcaaaaac gcccacaccg gtaaaaagtt ccagcggctt ggaacttctt
     7681 ggcgaaacag gcgttatggc cccgaaacga cgactgggca acgacgaccg ggcaatgatg
     7741 cctgggttac ggtcaggttg ttacatcgat atcgataaga ccaaaccggg ggtggttggg
     7801 gaaattatga aagaaaagac acaaggtccg gcagcgcgca acggctcgcg tggccgtttg
     7861 tcgggcgatc cgctgggggc aaattctgaa atcggccgaa aactcaggca atattacgac
     7921 gatctggtct ccgatgaggt gccggatcgc ttcacccagc tgcttgcgca gctggaacag
     7981 acagagcctc ctgtgaagaa gggataaggc atggccgtat cccaaggctt caagaccgat
     8041 ctgcttggct ccattccaag cctcagggcg tttgccatct cgttgacgca gagcgccgac
     8101 aaggccgacg atctggtgca ggaaacgctg gtaaaggcgt gggacaagca tgagagcttc
     8161 cagatgggca ccaacctcaa agcctggctg tttacgatcc tgcgcaacga attctattca
     8221 cagatgcgca agcgtggccg cgaggtgcag gacagcgacg gcatcatgac ggcgcgactg
     8281 gccgttcatc cttcccagca cgggcagctc gacctcaagg attttcgcgg cgcgctggaa
     8341 caactgcccg aagatcagcg cgaggcgatc atcctcatcg gcgcttccgg cttttcctat
     8401 gaggaagccg ccaagatttg cgactgcgcg gttggcacca tcaagagccg cgtcagccgt
     8461 gcgcgttcga aattgcagga aatcctgaaa ttatcgggcg aggaggacta tggccccgac
     8521 gcgatctcgg ctcaggtgat ggggtcgccg acaacctgat ccgtcggctg gcccatgggc
     8581 cgttcgacgg gcctgccgca gctggcggcc agggcctgga tgagatcgat gccggaatat
     8641 ggcttggtga tcatcgccac gccgggaaag ccggcttcaa tctccgccac gtcacaatag
     8701 ccggaggcga atatgaacgg cacttttcgc tcaaacaacg tctttgcgaa atcgagcgtc
     8761 gacacgccat ccagcatcag gtcgacaatt gcggcgtcga accggatggg caggccccgc
     8821 ccatccattt ccgccccatc taccccggcc ccggcctcac ctatcccggc cccgtctgca
     8881 ccggcaagac tgcgcaagat cgtcacttcg cgcgcgccgt agtcacggca aagctgttcg
     8941 acatccatgg cgatgagaaa ttcgtcttcg aggacgagaa tgtgcaggcc gtcgagcagt
     9001 tgagacacgt gcaggaaaca ctccattggc ggcagcattc atggccgata ttcctgcgtg
     9061 ggtcatttaa aaatgacgta gcaaaatcac gatgcgattc accccgaaac acatgcggga
     9121 ataatgtatc ctgtatcgcc gcgccgctct ggaaccttgg cgcataccgg acgttaaacc
     9181 gatagaaaac aaggagcatg caatggcact ggcagctgga aaaccggtaa acgaaggcgt
     9241 ggtcaattcc gacctcgaag ccgacatcgc acagttgaag gcagacatcg aaaaactgac
     9301 ccggcaattg gcggctaccg gagaacatgg ctatggcgcg gcgcgccgtg cggcggctga
     9361 aggtgtcgag aacctgcgcg tccagggcga ggccgcgctg gacagccttc gcggcagcgc
     9421 ccaggatatc gaggcgcagg tgatcgccag cgtgcgcgaa aagccggtga cttctctggc
     9481 catcgcggct ggcgtcggct ttctctttgc catgctcgcc cggcgctgac cgcgcatggg
     9541 aatgctggca tcgctgattg cgggattagc ctcgggcgaa actgccgccg ccattcgccg
     9601 cgcgcgcatg gcggctatgg tttatctggt tgcgggcgtg gccgcgctgt gcggcatcgg
     9661 cttcctgatc ggagccgctt tcctgtggac gtcctttcgc tacggttcta tccacgccgc
     9721 cctcggcttt ggcgtcggct tcctcgtgct ggccggcatt gtcgtgctgg cgttcaaact
     9781 tttttcgaac cggc
//
LOCUS       DASYCS010000344         7211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1108057,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000344 DASYCS010000000
VERSION     DASYCS010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 7211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7211
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108057"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(342..1403)
                     /locus_tag="VGM46_10805"
     CDS             complement(342..1403)
                     /locus_tag="VGM46_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007755227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4193122.1"
                     /translation="MAEINLMGVGHSYGGDAYALEKADLRLEDGKTYALLGPSGCGKT
                     TMLNIISGLIYPSDGRVVLGGKDVTRVKTAARNIAQVFQFPVVYQSKTVFENLAFPLR
                     CRNWESARIRARVNEVAELLGLTSRLHMSSRRLTADEKQLISLGRGLVRDDVAALLMD
                     EPLTVIDPQMKFELRRKIKQANDQTRHTVIYVTHDQNEAMTFADEIVVMKAGRVIQRG
                     TPQELFEKPYDTYVGNFIGSPGMNFIGVERHGDRLMLGEIEVLAGARVSALPASGKFT
                     IGIRPEHLAFEASSGCSIPAWLVSVQDHGKIRVLELALATSHIKMKIARDVSFPGTDI
                     RVFFSPDRIRLYADGIFVN"
     gene            complement(1391..2485)
                     /locus_tag="VGM46_10810"
     CDS             complement(1391..2485)
                     /locus_tag="VGM46_10810"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4193123.1"
                     /translation="MSLIIDNLHLAREGIPHLDGVTAEFGHGSITAVIGRTLAGKTTL
                     TRAIAGLQAVDGGSLRLDGKPFGGTPAWRRDVAMVYQQFINYPHLSVFENVAFPLRKR
                     KVAAGEIARRVTAALEMVGLASFGTRRPTELSGGQQQRVALARALVRRAGILLLDEPL
                     VNLDYKLREQLREEFRGLLKDQTGAIVIYNTTEPSEAMMLGNRIVVMHEGKVLQAGAP
                     AEIFDRPASVQVARIVNDPPMNIFSGIIENGAILLAGGLRIPVAEHLASLPPGSYRFG
                     IRAGELRVGGEGDASGKVTFSEVSGSETFVYAQAPFGDAVLQIEGVHAMANGTQLSIS
                     VAPDRLFAFSAEADGALLAAPVTGDAAWPK"
     gene            complement(2496..3338)
                     /locus_tag="VGM46_10815"
     CDS             complement(2496..3338)
                     /locus_tag="VGM46_10815"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY4193124.1"
                     /translation="MPGLYSSLKYLAVALWSVFVVTPLLWALTTSFKTSNAVTGGPTY
                     LPWLQYQPSLDGWRSLFGGAGGIDIVKPYMDSLIVTGSASLISIVLGTLAAYGLSRFD
                     YRAGFIKNTDIVFFFISQRIMPPIVLAIPFFLMLQFLGLLDSKIGLILVYIALLLPIA
                     VWIMVDFFDGVPRQIDEMAMLDGCTPFEAFLRVVLPNSLPGILVASMFCLVFGWNDFF
                     FAFTLTFTDTQLLPVAVVALNSSVTPWWSLSASALISVAPLILCAFLVERLLSRGTLA
                     GAIR"
     gene            complement(3338..4219)
                     /locus_tag="VGM46_10820"
     CDS             complement(3338..4219)
                     /locus_tag="VGM46_10820"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY4193125.1"
                     /translation="MDQSEFRFYKLSFLLPGFLVLLAIILFPLLFTIRVSLSGWDAIS
                     PGLDWAGVKNYAAVFGDIRFWQALWRLGGMAVFTVLAQYVLGFALALLVWREIRFRRF
                     FRVLFLIPMMTTPVIMSVIWRTIFHESLGPANDFLSLFGLGPYPWLTSANWALASVML
                     VEIWQWTPFMFLLLLAGLISLPREPFLAAAIDGAGPVRTFLNVTFPLLAPVSIGAITI
                     RLIEASKIMETVYVLTSGGPGTATETAGYYIYIKGLRDFEIGYAAALSITYLVIMIVL
                     MTIIAKLLVKLFVGGRA"
     gene            complement(4333..5868)
                     /locus_tag="VGM46_10825"
     CDS             complement(4333..5868)
                     /locus_tag="VGM46_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY4193126.1"
                     /translation="MASACMFAVTLGSAARAAEIDYSKYGDYAKYGSATDAPGACSYA
                     AIDAKDYSGRTLKVITHAVPVIGEPTDLHAKQFSDLTGAKVDVVHIPFGDLYQRIMIP
                     FQTGQAAYDVLFYGSLWIGDLHDFLAPVPAAYAEVAGMKDVTANFKDVATWGNQMVQY
                     PMDGDRHYMKVRSDIFDNPEMQAKYKKETGKDLKVPATWEDYNAVAKFFSGWDWAGDG
                     KPHFGSAEVTKRDDLMFSAFISRVAAYAKNPKVKGGFFFDLDTMEPQVNNPGFVKGLE
                     MFIDAQKAFPPGGSNFGLGDEIFSFGGGQTLMSYSWDDAFIQAQEKDSPIRNKVAAAP
                     LPGATEVWNRVTKSWDKFETPNAPPYITWGWTSAVSKASKNQDMAFDFLCFFSNEANA
                     SLDLQIGRFGINPYRKAHFDPNFWQDKLGWEPSVAQTYVKTLSAMDDSNNRVFDLRVP
                     GVNQFMSSMANGVAEAMAGQKTPQQALDDVAKQWSEIVDKIGKDKVRAAYANVVALED
                     RGR"
     gene            complement(5936..6988)
                     /locus_tag="VGM46_10830"
     CDS             complement(5936..6988)
                     /locus_tag="VGM46_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEY4193127.1"
                     /translation="MKAQVLNRYDDSLTADQWVTLQDVPDPKIEKSTDVIVRIGGAGV
                     CRTDLHIIEGVWRPFMDPNANDLLPLIMGHENAGWVEAVGKEVEGIKVGDPVIVHPKI
                     SGGTCLACRRGFDMHGPGKFPGLDTNGGYAEYLCTSERNIVPLPKTLAPKDVAAYADA
                     GLTAYRAVKKATRHLLPGENCVVIGAGGLGHIGIQCLKAMCAADIIVVDKSDASLELA
                     GKSGADRLVKADGNEVEAVLALTDGQGAEAVIDFVGERGTTTKGLAMTRAMGSYYVVG
                     YGEEIRIPTVEMVITEKNIIGNLVGNWAELVELMALADRGLVELTTVEYRLADANRAL
                     RDLHEGKIHGRAVLIP"
BASE COUNT         1367 a         2184 c         2326 g         1334 t
ORIGIN      
        1 tttggtgtgt tgcgttttga tgatgcattc atggcaggac caaggcccat caagcgcaag
       61 tcgctgaagg cgtggctgcc atgacctgtg cgcaagtgcg agcgcccgga tggccggcac
      121 attggatcgg cgagggccgg aaatgaaatg gcttctggca atcgaattaa tagccgtgcg
      181 ttatgctgcg cgcacgatca cgctgaccgt gtccggttgc cacggaagtt tccgccgccg
      241 atatgcgggt cagttgccga gcgcgttcag ccgttcaacc catcggttcc cacgacaaat
      301 ctcgtgcctg atggtcgtga cgaaagtgcg ggtctgaaac ctcaattgac gaagatgccg
      361 tcggcataaa gcctgatcct gtcgggcgag aagaacaccc ggatgtcggt tccgggaaaa
      421 gaaacgtcac gcgcgatctt catcttgatg tggctggtcg ctaaagcgag ttcgagcacg
      481 cgaatcttgc catgatcctg aacggagacc agccatgccg ggatgctgca cccagagctt
      541 gcctcgaagg ccagatgttc cggtctgatc ccgatcgtaa acttgccgct tgcgggcagg
      601 gcgctaacgc gcgctcccgc cagaacctcg atctccccca gcatgaggcg gtcgccgtgc
      661 cgctcgacgc cgatgaagtt catgcccggt gagccgatga aattgccgac ataggtatcg
      721 tatggcttct caaagagttc ctgcggcgta ccgcgctgga tgacgcggcc cgccttcatc
      781 accacgattt cgtcggcgaa agtcatggcc tcgttctggt catgcgtgac atagatcacc
      841 gtgtgcctgg tctggtcgtt ggcctgcttg atcttgcggc gaagctcgaa cttcatctgc
      901 gggtcgatga ccgtcagcgg ctcgtccatc aacagcgccg ccacgtcgtc gcgaaccagg
      961 ccgcgcccaa gcgaaatcaa ctgcttttcg tcggcggtca gacggcgcga cgacatatga
     1021 agccgcgacg tcaggccgag aagctccgca acctcgttca cccgcgcgcg tatccttgcc
     1081 gattcccaat tgcggcagcg tagcgggaag gcaaggtttt cgaagaccgt cttcgactga
     1141 tagacgaccg ggaactggaa aacctgggcg atgttgcgcg ccgccgtttt aacgcgggtc
     1201 acgtccttgc cgcccagcac cacccgtcca tcggatgggt agatcagccc ggaaataatg
     1261 ttgagcatcg tcgtcttgcc gcagccggag gggccaagca gggcgtatgt cttgccgtct
     1321 tcgagccgca ggtcggcttt ctccagggca taagcgtcgc cgccatagga atggccgacg
     1381 cccatcaggt ttatttcggc catgcggcgt ctcctgtgac cggggctgcg agcagcgcgc
     1441 cgtcggcttc ggcggaaaag gcaaacagcc ggtccggcgc gaccgagatg gaaagctggg
     1501 tgccgttcgc catggcgtgg acgccctcga tctgcaacac ggcgtcgccg aacggcgctt
     1561 gcgcatagac gaaggtttcc gaaccggaga cttccgagaa cgtgaccttg cccgatgcgt
     1621 cgccctcgcc gccgacgcgc agttcgccgg cgcgaatgcc gaagcggtac gagccgggcg
     1681 ggaggcttgc aaggtgttcg gcaaccggga tgcggaggcc gcctgcgagc aggatggcgc
     1741 cgttctcgat gatgcccgag aagatgttca tcggcggatc gttgacgatc ctggcgacct
     1801 gcacggaagc gggacggtcg aatatctcgg ccggcgcgcc cgcttgcagc accttgcctt
     1861 catgcatgac cacgatgcgg ttgcccagca tcatcgcctc ggacggctcg gtcgtgttgt
     1921 agatgacgat ggcgccggtc tggtctttca gcagcccgcg aaattcctcg cgcaattgtt
     1981 cgcgcagctt gtagtcgaga ttgaccagcg gctcgtcgag cagcaggatg ccggcccttc
     2041 gcaccagcgc gcgggcaagc gccacgcgct gctgttgccc gccggaaagc tccgtcggcc
     2101 ggcgtgtgcc gaagctggca agcccgacca tctccagcgc ggcggtgacg cggcgcgcga
     2161 tctcgccggc ggcgaccttg cgcttgcgca gcgggaaggc gacgttctcg aagacgctca
     2221 gatgcggata gttgatgaat tgctggtaga ccatggcgac gtcgcgccgc catgccggcg
     2281 tgccgccgaa cggcttgccg tcaagtcgca ggctgccgcc gtcgaccgcc tgtagcccgg
     2341 cgatggcgcg cgtcagcgtg gtcttgccgg ccagcgtgcg cccgatcacc gcggtgatgc
     2401 tgccatgccc gaactcggcg gtgacgccgt ccaggtgggg tatgccctcg cgcgcaagat
     2461 gcagattgtc gatgatgagg ctcattttgc gttcctcacc gaatggcgcc ggccagcgtg
     2521 ccacgcgaca aaagccgctc gaccaggaag gcgcacagga tcaatggcgc gaccgagatg
     2581 agggccgatg ccgacaggct ccaccagggc gttaccgacg aattgagcgc caccaccgct
     2641 accggcagaa gctgcgtgtc ggtgaaggtc aaggtgaagg cgaagaagaa gtcgttccag
     2701 ccgaagacca ggcagaacat ggaagcgacc aggatgccgg gcagggaatt gggcagcacc
     2761 acgcgcagga atgcctcgaa gggcgtgcag ccgtccagca tcgccatctc gtcgatctgg
     2821 cgcggcacgc cgtcgaagaa gtcgaccatg atccacaccg cgatcggcaa aagcagcgcg
     2881 atatagacga ggatgaggcc gatcttcgaa tcgagcagcc ccaggaattg cagcatcagg
     2941 aaaaacggaa tggccagcac gatgggcggc atgatgcgct gggagatgaa gaagaacacg
     3001 atgtcggtat tcttgatgaa gccggcgcgg taatcgaagc gtgagaggcc ataggcggcg
     3061 agcgtgccca ggacgatgga gatgaggctg gcgctgccgg tcacgatcag gctgtccata
     3121 tagggcttga cgatatcgat gccgcccgcg ccgccaaaca ggctgcgcca gccgtccagc
     3181 gacggctggt attgaagcca cggcaggtag gtcgggccgc cggtaaccgc gttggaggtc
     3241 ttgaagctgg tggtcagcgc ccacagaagc ggcgtcacca cgaacacgct ccacagcgcc
     3301 accgccagat atttcaacga agaataaagg ccgggcatca ggcgcgccct cccacgaaca
     3361 gcttgacgag aagcttggcg atgatggtca tgagcacgat catgatgacg aggtaggtga
     3421 tggagagcgc cgccgcatag ccgatttcga agtcgcgcag gcccttgatg tagatgtagt
     3481 agccggccgt ctcggtggcc gtgcccggcc cgccggacgt caatacgtag accgtctcca
     3541 tgatcttcga cgcctcgatc agccggatgg tgatggcgcc gatggagacg ggcgccagga
     3601 gcgggaaggt gacgttgaga aaggtgcgga ccggtcccgc gccatcgatg gcggcggcga
     3661 ggaaaggctc gcgcggcagg cttatcagcc cggccagcag caacaggaac atgaaaggcg
     3721 tccattgcca tatctcgacc agcatgacgc ttgccagcgc ccagttggcc gaggtcagcc
     3781 agggataggg gccgaggccg aacaggctga ggaagtcgtt ggccggcccc agcgattcgt
     3841 ggaagatggt gcgccagatc accgacatga tgacgggcgt cgtcatcatc gggatgagga
     3901 acagcacgcg gaagaagcgg cgaaagcgga tttcgcgcca taccagcagc gccagcgcga
     3961 agcccagcac atactgggcc aggacggtga agaccgccat gccgccgagc cgccacagcg
     4021 cctgccagaa gcgtatgtcg ccgaacacgg cggcgtaatt cttgacgccg gcccagtcca
     4081 ggccgggcga tatggcatcc cagccggaga gcgagacgcg gatggtgaac aacaacggaa
     4141 acaggataat cgccagcagg accagaaagc ccggcagcag gaacgacagc ttgtagaaac
     4201 gaaattcgga ctggtccatc tggtcctttg acttctgctg gagatgcgcg acgcggcccc
     4261 tggcgcggat acggaagacg gggcgatgac cccgtcttcc tctatttccc ggtcttcgtt
     4321 acaaagaccg gatcagcggc cccggtcttc cagtgccacc acgttggcat aggcggcgcg
     4381 caccttgtcc ttgccgatct tgtcgacgat ctcgctccac tgcttggcaa cgtcgtcaag
     4441 cgcctgctgc ggcgtcttct ggccggccat cgcctcggca acgccgttcg ccatcgagga
     4501 catgaactgg ttcacccccg gcacgcgaag gtcgaacacg cggttgttgc tgtcgtccat
     4561 ggccgagagc gtcttgacat aggtctgcgc cacgctcggc tcccagccca gcttgtcctg
     4621 ccagaagttc ggatcgaaat gcgccttccg gtagggattg atgccgaagc ggccgatctg
     4681 gaggtcaagc gaggcattcg cctcgttgga gaagaagcac aggaagtcga aggccatgtc
     4741 ctggttcttc gaagccttgg aaacggcgga agtccaaccc caggtgatgt agggcggggc
     4801 gttcggcgtt tcgaacttgt cccagctctt ggtcacgcgg ttccacactt cggtagcacc
     4861 cggcagcgga gcggcggcga ccttgttgcg gatcgggctg tccttttcct gcgcctggat
     4921 gaaagcgtcg tcccatgaat agctcatgag ggtctgcccg ccgccgaacg agaagatctc
     4981 gtcgccgagg ccgaaattcg aaccgccggg cgggaaggcc ttctgcgcgt cgatgaacat
     5041 ttccaggccc ttgacgaagc ccggattgtt gacctgcggc tccatggtgt cgaggtcgaa
     5101 gaagaagccg cccttgacct tcgggttctt ggcataggcg gcgacgcggc tgatgaaggc
     5161 cgaaaacatc aggtcgtcgc gcttggtgac ctcggcgctg ccgaaatgcg gcttgccgtc
     5221 gccggcccag tcccagccgg agaagaattt cgcaaccgcg ttatagtctt cccaggtcgc
     5281 cgggaccttg aggtccttgc cggtctcctt cttgtatttc gcctgcatct ccggattgtc
     5341 gaaaatgtcg gagcggacct tcatgtagtg gcggtcgcca tccatcggat attgcaccat
     5401 ctggttgccc caggtggcca catccttgaa gttggccgtc acgtccttca tgcccgccac
     5461 ttcggcgtag gcggcgggaa ccggcgcgag gaaatcgtgc aggtcgccga tccacagcga
     5521 gccgtagaac aaaacgtcat aggcggcctg cccggtctgg aacgggatca tgatgcgttg
     5581 gtagaggtcg ccgaagggaa tgtggacgac atcgaccttc gcgccggtca ggtccgaaaa
     5641 ttgtttggcg tgcaggtccg tcggttcgcc gatgaccggc acggcatggg tgatcacctt
     5701 cagcgtgcgg ccggaatagt ccttggcgtc gatcgccgcg taggaacagg cgccgggtgc
     5761 atcggtggcg ctgccgtatt tggcgtagtc gccatatttg gaatagtcga tctcggccgc
     5821 gcgcgcggcc gagccgagcg tcaccgcgaa catgcaggcc gacgccatga gggctgacgc
     5881 cctccgtagt gcttttttct tcatactgct tcctccacct tgatagcttc gtcgttcagg
     5941 gaatgagcac ggcgcggccg tggatcttgc cttcgtgcag gtcgcgcagc gccctgttgg
     6001 cgtccgcaag ccggtattcg acggtcgtca gttcgacgag gccgcgatcg gccagcgcca
     6061 tcagctcgac cagctcggcc cagttgccga cgaggttgcc gatgatgttc ttttcggtga
     6121 tgaccatctc gacggtcggg atgcggattt cctcgccgta gccgacgaca tagtagctgc
     6181 ccatcgcacg ggtcatggcc aggcccttgg tggtcgtgcc gcgttcgccg acgaagtcga
     6241 tgaccgcctc ggcgccctgg ccgtcggtca gcgccagcac ggcttcgacc tcgttaccgt
     6301 ccgccttgac cagcctgtcg gcgccgcttt tgccggcaag ctccagcgag gcgtcggatt
     6361 tgtcgacgac gatgatatcg gccgcacaca tggccttcag gcactggatg ccgatatggc
     6421 cgaggccgcc agccccgatg acgacgcagt tctcgccggg cagcaagtgc cgggtcgctt
     6481 tcttgaccgc gcgataggcc gtcaatccgg catcggcgta ggcggcgaca tccttcggcg
     6541 cgagcgtctt gggcaagggc acgatgttgc gctcggaggt gcacagatat tccgcgtagc
     6601 cgccattggt atcgaggccg gggaatttgc cggggccgtg catgtcgaag ccgcgccggc
     6661 aggccaggca tgttccgccc gaaatcttgg gatgcacgat gaccggatcg ccgaccttga
     6721 tgccttccac ttccttgccg accgcctcta cccagccggc gttctcatgc cccatgataa
     6781 gcggcagcag gtcgttggca ttggggtcca tgaacgggcg ccacacgcct tcgatgatgt
     6841 gcaggtcggt gcggcacacg ccggcaccac cgatgcgcac gatcacatcg gtggacttct
     6901 cgatcttcgg gtcgggtaca tcttgcaacg tgacccactg gtcggccgtg aggctgtcgt
     6961 catacctgtt cagtacctga gctttcatgc ttatctccct gggcgccgtc atgagcccgt
     7021 cattcgggat gaaggacgca atagccgtgc cagatggtga agcttattag aaacaatggc
     7081 ttgactgcat accgcaatgg cccggccgta cagcggctgg acatttgacc gaccgggttg
     7141 ttcagtttct ggttattgca gtttcgacgt ttgcgcccat actgcgcccg ctaatatatt
     7201 tcgggaggaa t
//
LOCUS       DASYCS010000345         4577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2704551,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000345 DASYCS010000000
VERSION     DASYCS010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2704551"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="VGM46_10835"
     CDS             complement(<1..688)
                     /locus_tag="VGM46_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY4193128.1"
                     /translation="MPFDRQTTLASPTGALLNLYVRQAQGAGRAVVQINHGLAEHALR
                     YARFADFLAQHGFHAYAHDHRGHGFTTAPDAPLGRFAQTGGGGKVIADVAAVHDLIAR
                     EHPGLPVIVFGHSMGGLIALNFVLRHSTRVQAAAIWNANFSAGLAGRAALAILAWERF
                     RLGSDVPSQLLPKLTFQAWGKAVPNHATPFDWLSRDAAEVQKYIADPLCGFNASVSLW
                     RDLFGLIFDGA"
     gene            complement(778..2052)
                     /locus_tag="VGM46_10840"
     CDS             complement(778..2052)
                     /locus_tag="VGM46_10840"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019859142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY4193129.1"
                     /translation="MHNVFLLFAEAVLYFGIMGTLFGLRNRIGLGVFVCALGVMHFLE
                     TYLASVFYVALPFGMVSPGSAVLFSGKLALLLLLYMKEDAATVRQPIYGLLLGNALMI
                     GLVLILRQHDISPLPDGQRPDIGFIDQMGWLMVWGTTLLFADAILLILLYERLGRYFR
                     KALFLRILVSVACVLTFDQAGFFTALHFVAGAPAEVFFGGWFAKMGAALTYSVMLCAY
                     LKYFEGRHIATQRGLVDIFETLTYRERYEALVEQVGRDGLTGLLHRGRFETDAQQAIA
                     KNIESAKPLSLLVLDVDHFKSINDRFGHAEGDRVLKTIAALLTSAAGREAQIYRIGGE
                     EFAIICAHPHQVARLLGENIRHVIKTSFMSGTPEITVSIGVATISVAIAHLDSLFGIA
                     DQRLYIAKSTGRDRVVGDNAEEEAAISAPLAS"
     gene            2324..3973
                     /gene="ettA"
                     /locus_tag="VGM46_10845"
     CDS             2324..3973
                     /gene="ettA"
                     /locus_tag="VGM46_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018095480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEY4193130.1"
                     /translation="MARQFIYHMAGLNKAYGTKKVLENVHLSFYPDAKIGILGPNGAG
                     KSTILKIMAGIDKEWSGEAWLAEGATVGYLAQEPALDPALDVFANIMEGVAAKTAIIE
                     RYNELMMNYSDETAEESAKLQDEMDRLNLWDLEQQVEMAMEALGCPPKDADVTKLSGG
                     ERRRVALCQLLLREPDLLLLDEPTNHLDAETTAWLEKHLRAYKGAVMIITHDRYFLDN
                     VTGWILELDRGRSIPYEGNYTKYLEAKAKRLKQEGREDDSRQRAIGREREWIQSSPKA
                     RQTKSKARIQAFQDLLDAADKRRPSDTQIVIPHGERLGNVVIEVDGLNKGFGEELLIE
                     DLAFKLPPGGIVGVIGPNGAGKTTLFRMITGQEQPDAGSVRIGETVKLGYVDQSRDTL
                     DPAKTVWEEISGGAEVVKLGKFEANTRAYCASFNFRGGDQQQKVGNLSGGQRNRVHLA
                     KMLKTGGNVLLLDEPTNDLDTETLAALEDALENFGGCAVIISHDRMFLDRLATHILAF
                     EGDSHVEWFEGNFEDYEQDKIRRLGSEAVNPHRMTYKRLTR"
     gene            4043..>4577
                     /locus_tag="VGM46_10850"
     CDS             4043..>4577
                     /locus_tag="VGM46_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY4193131.1"
                     /translation="MPDWSAAQYLKFEDERTRPARDLLAQVPVAKPRRVVDIGCGPGN
                     STELLVERWPQAQVRGFDTSPDMISKAKARLPGVSFELMDAEAWSPAEPVDVIFANAV
                     FQWLPQHPALLQRLMGFLAPGGVLAVQMPDNLGEASHRLMREAALEMPFAQKMAKSPR
                     KPLPPVSLYYDLLAPLCE"
BASE COUNT          948 a         1400 c         1463 g          766 t
ORIGIN      
        1 cagcgccatc gaagatgagg ccaaacaggt cgcgccacag ggacaccgaa gcattaaagc
       61 cgcagagcgg atcggcgatg tatttttgca cttccgccgc gtcgcgcgac agccagtcga
      121 acggcgtcgc gtggttcggc accgccttgc cccatgcctg gaaggtaagc tttggcaaca
      181 gctgcgacgg cacgtccgag ccgaggcgaa atcgctccca ggcgagaatg gcaagtgctg
      241 cgcggccagc cagcccggcc gagaaattgg cgttccagat ggccgccgcc tggacgcggg
      301 tggaatggcg cagcacgaaa ttcagcgcga tgaggccgcc catcgaatgg ccgaagacga
      361 tgaccggcag gccgggatgt tccctggcga tcaaatcatg cacggcagcc acatcggcaa
      421 tcaccttccc gccaccgcct gtctgggcaa accggcccag cggcgcgtcc ggcgcggtgg
      481 tgaaaccgtg gccgcgatgg tcgtgcgcat aggcatgaaa gccgtgctgg gcgaggaagt
      541 cggcaaagcg ggcatagcgc aacgcgtgtt cggccaggcc atggttgatc tgcactacgg
      601 cgcggcccgc accttgcgcc tgccggacat agaggttgag aagcgcgccg gtgggcgaag
      661 cgagtgtcgt ctggcgatcg aacggcatgc ggctttcccc tcccgatggc cgactgttgg
      721 cgatggcaaa ggcatcgtca agcatcggcc cgcccttcat gcggcgacgt gttgcgttca
      781 gcttgcgagc ggcgcggaaa tcgcggcttc ttcctcggca ttatcgccga cgacgcggtc
      841 tcgccccgtc gatttggcga tatagagccg ctggtcggca atgccgaaca ggctgtcgag
      901 atgggcgatg gccaccgaga tcgtcgccac gccgatgctg acggtgattt ccggcgtgcc
      961 gctcatgaag gatgtcttga tgacatggcg gatgttttcg cccagcagtc gcgccacctg
     1021 atgcggatgc gcgcatataa tggcgaattc ctcgccgccg atgcggtaga tctgggcctc
     1081 gcgcccggcc gcgctggtca gcagggcagc tatggtcttc agcacgcggt cgccctcggc
     1141 atggccgaag cggtcgttga tcgacttgaa atgatcgaca tcgagaacca gcaggctaag
     1201 cggctttgcg ctttcgatgt tttttgcaat cgcctgctgg gcgtccgttt cgaagcggcc
     1261 tctgtgaagg aggcccgtca ggccgtcgcg tccgacctgt tccaccagcg cctcgtagcg
     1321 ctcgcggtaa gtcagggttt cgaagatatc gacaaggccg cgctgcgtgg cgatatggcg
     1381 gccctcgaaa tatttgagat aggcgcacag catgacgctg taggtgagcg cggcgcccat
     1441 cttcgcaaac cagccgccga agaacacctc ggcaggcgcg ccggcgacga agtgcaaggc
     1501 cgtgaagaag cctgcctggt cgaatgtcag cacgcaggcg accgagacca gaatgcgaag
     1561 gaacagcgcc ttgcggaaat accggcccag ccgttcgtaa agcaaaatga gcaggatggc
     1621 gtcggcgaaa agcagcgtgg ttccccacac catcagccag cccatctggt cgatgaagcc
     1681 gatatccggg cgctgcccat cgggaagcgg cgagatgtca tgctggcgca agatcagcac
     1741 cagcccgatc atcagcgcat tgccgagcaa caggccgtag atcggctggc gcacggtggc
     1801 ggcgtcctct ttcatgtaga gcagcagcaa caaggccagc ttaccggaaa acagcacagc
     1861 cgagccgggc gaaaccatac cgaatggcag cgcaacgtag aatacgctgg caaggtaggt
     1921 ttcgaggaaa tgcatgacgc ccagcgcgca gacgaaaacg ccaagcccga tgcggtttct
     1981 caggccaaac agagtgccca tgatgccgaa ataaagcacg gcctcggcaa agagcaggaa
     2041 gacattgtgc aagcttgcgg ctccgctggt ggtcgaccgc gccccaaaaa ggcgctcttg
     2101 cttccacttc ggttttaaca agcgagagtt aatcggccct tatgcacagg ggagacgcca
     2161 cgaatgccgg gcaggcccga ctttttcatc agcgatgcgg gggcggggat gcgcagggtg
     2221 gggacgcgcc aaaaccgatt gccgtttgcc gccctatcgc ctacataacc cgcaacctcc
     2281 acccaattcg actttcacag tcacttgtac agggaacgcg cgcgtggcac gccaattcat
     2341 ctatcacatg gccggcctca ataaggccta cggcaccaag aaggtgctcg aaaatgtcca
     2401 tctttctttc tatcccgatg ccaagatcgg cattctcggc cccaacggcg cgggtaagtc
     2461 gaccattttg aagatcatgg ccggcatcga caaggaatgg agcggcgagg catggctcgc
     2521 cgagggagcc accgtcggct acctggcaca ggaaccggcg ctcgacccgg cgctcgacgt
     2581 gttcgccaat atcatggaag gcgttgccgc caagaccgcc atcatcgagc gctacaacga
     2641 attgatgatg aactattccg acgagacggc cgaagaatcg gccaagctcc aggacgagat
     2701 ggaccggctc aacctgtggg acctcgaaca gcaggtcgag atggcgatgg aagcgctggg
     2761 ctgcccaccc aaggatgccg acgtcaccaa gctttccggc ggcgagcgcc gccgcgtggc
     2821 gctgtgccag ctgctgctgc gcgagccgga cctgcttttg ctggacgagc cgaccaacca
     2881 tctggacgcc gagacgaccg catggctgga aaaacatctg cgcgcctata agggcgcggt
     2941 gatgatcatc acccacgatc gctacttcct cgacaatgtc accggctgga ttctggaact
     3001 ggatcgcggc cgcagcattc cttacgaggg caattacacc aaatatctgg aagccaaggc
     3061 caagcgcctc aagcaggaag gccgggagga cgattcgcgc cagcgcgcca tcggccgcga
     3121 gcgcgagtgg atccagtcca gcccgaaggc gcgccagacc aaatccaagg cgcgcatcca
     3181 ggcgttccag gatttgctgg atgcagccga caagcggcgt ccaagcgaca cgcagattgt
     3241 tattccgcat ggcgagcggc tcggcaatgt cgttatcgaa gtcgacggcc tcaacaaggg
     3301 cttcggcgag gagttgctga tcgaagatct ggccttcaag ctgccgcccg gcggcattgt
     3361 cggggtgatc gggccgaacg gcgccggcaa gacgacgctt ttccgcatga taaccgggca
     3421 ggaacagccg gatgccgggt ccgtgcgcat cggcgagacg gtcaagcttg gctatgtcga
     3481 ccaaagccgc gatacgctcg acccggccaa gaccgtgtgg gaggaaattt cgggtggggc
     3541 cgaagtggtc aagctcggca agttcgaagc caatacgcgc gcttattgcg cgtcgttcaa
     3601 cttccgtggc ggcgaccagc agcagaaggt cggcaacctg tcgggcggcc agcgcaaccg
     3661 cgtgcatctg gccaagatgc tgaagacggg cggcaacgtg ctgctgctcg acgaaccgac
     3721 caacgatctc gacaccgaaa cgctggcggc gcttgaagat gcgctggaga atttcggcgg
     3781 ctgcgcggtc atcatttcgc atgatcgcat gttcctcgac cgtcttgcca cgcatatcct
     3841 ggccttcgag ggcgacagcc atgtcgaatg gttcgaaggc aatttcgagg attacgagca
     3901 agacaagatc cgccgcctcg gctccgaagc cgtcaacccg caccgcatga cctacaagcg
     3961 cctgacgcgg taagatcgcc tgcaattcgc ccggccgtca cccgatgcgg ccaggcgaat
     4021 gatcgaacaa gaggaacatc gaatgcccga ttggtccgcc gcgcagtatc tcaaattcga
     4081 ggacgagcgg acgcggccgg cgcgggattt gctggcgcag gtgccggtag ccaagccgcg
     4141 ccgtgtggtc gacataggct gcgggccggg caattcgacg gagcttctgg tcgagcgttg
     4201 gccgcaggcg caggtccggg ggttcgacac ctcgcccgac atgatctcca aggcgaaggc
     4261 gcggcttccc ggcgtctcgt tcgaactgat ggatgccgag gcgtggtcgc cggccgagcc
     4321 ggtcgacgtg atcttcgcca acgcggtatt ccagtggctg ccgcagcatc cggccttgct
     4381 ccagaggctg atgggatttc ttgcgcccgg tggcgtgctg gcggtgcaga tgcccgacaa
     4441 tctcggcgag gcctcgcacc ggttgatgcg ggaagccgcg ctggaaatgc cgtttgccca
     4501 aaaaatggcg aagtcgccga gaaagccact gccgcccgtc tcgctctatt acgatctgct
     4561 ggccccgctc tgcgagc
//
LOCUS       DASYCS010000346         3212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2704950,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000346 DASYCS010000000
VERSION     DASYCS010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2704950"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="VGM46_10855"
     CDS             <1..647
                     /locus_tag="VGM46_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193132.1"
                     /translation="PDPVEWAYFLLSMICTGELGQARRIRDFYPGLGHEELVRARHVL
                     DWLADGVSQDAQTDHLPKHRKSIHRLESRPDGEWLAWFATILDRCRQPELAARLRDLS
                     AASLSGKAQPRRTGVGAATGWRNVAYASADRLIAISGLGALRPNVPPMAEFRYFERVA
                     RNFGRSIFRGRLRPLGLALSRLATAMREVGPAERIRVLAGVPTPRPTHPPDTPR"
     gene            644..2140
                     /locus_tag="VGM46_10860"
     CDS             644..2140
                     /locus_tag="VGM46_10860"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEY4193133.1"
                     /translation="MNTVRQSIAFSMVEKYLSQILLIATTAVMARILTPAETGLYMTA
                     NAVVLLADNFRTFGVGVYIVQEKTLSRTLVRSAFTVTLALSLAMGAAIWLGAGSLAAF
                     YATPELKTLLMVSALGFLAIPFSSPIMALLQRDLAFRAIACINLSAAAAGFVTTVTLG
                     LAGWGPASYAWGFVGASFVMGALAFAARPHFWIFRPCLDGARQLLSFGSISSLITVSN
                     MGYELLPRLAFGKILGFDAVGLYSRAITVCQLPDRVVVSALQPVVLPAFAAHARAGGS
                     LKQSYLHGLSLMSAVQWPALILLSLLANPVVHVLLGPQWGEVPPLVRIMALANMALAP
                     AVLTFPALVSSGRIGDALWSSLISLPPSFLIVTGAAYYFGLSAVAASLFVVAPMQMAV
                     AYFFIRRAIDLSWAELTHAARDSALLAAGTAVLPIAVVLTSPSGFDLDWVRTLAAIVG
                     AACGWLAALALVDHPVKREVAGAWRFFRESMTRPRMAANPRRMAPETK"
     gene            2173..3168
                     /locus_tag="VGM46_10865"
     CDS             2173..3168
                     /locus_tag="VGM46_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016734704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HEY4193134.1"
                     /translation="MGEPYSLSGKRVWVAGHRGLVGSALVRRLGRENIELLTAGRDRI
                     DLRRQQETEAWMMRERPQVVFLAAAKVGGIHANDRRPADFIYDNLMIEANVIHAALLA
                     GVEKLALLGSTCIYPRLAPQPIPEEALLTGPLEPTNEWYAVAKIAGIKLAQAYRRQHG
                     CDFISAQPTNLYGPGDNFDLETSHVLPALMRKAHDAKQDGAASMTVWGSGTPLREFLH
                     VDDLADALVFLAQHYSGEGPVNVGSGVEISIRDLADAIRKAVGFEGQLMFDAAKPDGT
                     PRKLADISKLAAMGWRHRIGLDEGLASTYDWFLNHAAAVHKPAEERLAPLLRSRS"
BASE COUNT          502 a         1066 c         1021 g          623 t
ORIGIN      
        1 accccgatcc cgtggaatgg gcctattttc tgctttccat gatttgcacg ggcgagcttg
       61 gccaggcgcg gcgcatacgg gatttctatc cgggccttgg gcatgaggaa cttgtgcggg
      121 cgcgccacgt tctggactgg ctggccgatg gcgtctcgca ggacgctcaa accgatcatc
      181 tgccaaagca ccgaaaaagc atccaccggc tggaaagccg ccccgacggc gaatggctgg
      241 catggttcgc cacaatcctc gaccgttgcc gccagcccga actcgccgca agactgcgcg
      301 acctttccgc cgccagcctt agcggcaagg ctcagccccg ccgaaccggc gttggcgctg
      361 caacaggctg gcgaaacgtg gcctatgcca gcgctgaccg gctgatcgcc atcagcggcc
      421 ttggcgcgct gcgtccaaat gtgccgccaa tggccgaatt ccgctatttc gagcgggtgg
      481 ctcggaattt cggccggagc atttttcgcg gccgtctgcg gcctttgggc ctcgcgctgt
      541 cacggcttgc caccgctatg cgggaagtcg gcccggcaga acggatcagg gttttggccg
      601 gcgttccaac accgcgtccg acacatccgc cggacacgcc ccgatgaaca cggtccggca
      661 atccatcgct ttttcgatgg tcgaaaaata cctgtcgcaa atcctgctga tcgcaacgac
      721 ggcggtgatg gcgcgcattc tgacgccggc ggaaaccggc ctctacatga cggccaatgc
      781 ggtggtcttg ctggcggata atttccgcac cttcggcgtc ggcgtctata tcgtgcagga
      841 aaaaaccctg agccgcacgc tggtgcgctc ggccttcacg gtcacgctgg cgctgtcgct
      901 ggccatgggc gcggcgatct ggcttggcgc cggctcgctc gccgcctttt atgcgacgcc
      961 ggagttgaaa accctgttga tggtttcggc actcggcttc ctcgccattc ccttcagcag
     1021 cccgatcatg gcgctgcttc agcgcgacct cgccttccgg gccatcgcct gcatcaacct
     1081 ttccgccgcc gccgccggct tcgtcaccac agtgacgctc ggcctggcgg ggtggggacc
     1141 ggccagctat gcgtggggct tcgtcggcgc cagcttcgtc atgggagcgc tggcgttcgc
     1201 cgcgcggccg catttctgga ttttccgtcc ctgcctggac ggtgcgcgcc agttgttgtc
     1261 cttcggctcg atctcctcgc tgatcaccgt atcgaatatg ggctacgagc ttttgcctcg
     1321 gctggccttc ggcaagatac tgggcttcga cgccgtcggc ctctattccc gcgcgatcac
     1381 ggtttgccaa ttgcccgacc gcgtcgtcgt ttcagcgctc cagccggtgg tgctgccggc
     1441 ctttgccgcc catgcgcgcg ccggcggctc gctcaagcaa agctacctgc atggccttag
     1501 cctgatgtcg gcggtgcagt ggccggcgct gatcctgctc tcgctgcttg ccaacccggt
     1561 ggtccatgtc ctgctcgggc cgcaatgggg agaagtgccg ccgctggtgc gcatcatggc
     1621 gctggccaac atggcgctgg cgccggcggt gctgaccttt cccgcgctcg tctcctccgg
     1681 ccgcatcggc gatgcgctgt ggtccagcct catcagcctg ccgccttcgt tcctgatcgt
     1741 gaccggcgct gcctattatt tcggccttag cgccgttgcg gcaagccttt tcgtcgtcgc
     1801 gccgatgcag atggccgtgg cctatttctt catccgccgc gccatcgacc tgtcatgggc
     1861 cgaactgacg catgcggcgc gcgacagcgc cttgcttgca gcaggcacgg ctgtcttgcc
     1921 gatcgcggtg gtgctgactt cgcccagcgg tttcgatctc gactgggtgc gcacgcttgc
     1981 cgcaatcgtc ggcgccgcct gcggctggct cgccgcgctg gcgcttgtcg accatccggt
     2041 gaaaagggaa gtggccggcg cttggcgttt ttttcgggaa agcatgacgc ggccgcgcat
     2101 ggcggccaac ccgcgccgca tggcgcccga aacgaaatag cgcataatga gcaaatggcg
     2161 gagacgcggc gaatgggcga accttacagt ctctcgggca agcgggtctg ggtcgccggc
     2221 catcgcgggc tggtcgggtc ggcgctggtc cgccggcttg gccgcgaaaa catcgaactg
     2281 ctgaccgcag gccgagacag gatcgacctg cgccgccagc aggaaaccga ggcctggatg
     2341 atgcgggaaa gaccgcaggt ggtgtttctc gccgccgcga aggtcggcgg catccatgcc
     2401 aatgaccgcc gccccgctga tttcatctac gacaatttga tgatcgaggc caatgtgatt
     2461 catgcggcgt tgcttgccgg cgtggagaag ctggcattgc tgggttccac ctgcatctat
     2521 ccgcgcctgg cgccacagcc gatccccgag gaggcgctgc tgaccggccc tcttgagccg
     2581 acgaacgaat ggtatgcggt ggccaagatc gccggcatca agcttgccca ggcctaccgg
     2641 cggcagcatg gctgcgactt catctcggcg caaccgacca acctctacgg tccgggcgac
     2701 aatttcgatc tcgaaaccag ccacgtcctg ccggcgctga tgcgcaaggc gcatgacgcg
     2761 aaacaggacg gcgccgcgag catgaccgtg tgggggtccg gcacgccttt gcgcgaattc
     2821 ctgcatgtcg acgaccttgc cgacgcgctg gttttcctcg cgcaacatta cagcggcgag
     2881 gggccggtca atgtcgggtc gggcgtggag atttcgatcc gcgatcttgc cgacgccatc
     2941 cgcaaagcgg tcggcttcga agggcagctg atgttcgacg ccgccaagcc cgatggcacg
     3001 ccgcgcaagc tggcggatat ttccaagctt gccgcaatgg gctggcgcca tcgcatcggc
     3061 ctcgatgaag gcctcgcatc gacctatgac tggttcctca accacgctgc ggcagtacat
     3121 aagcccgctg aggaacgctt ggcgccgctg ctgcggtcga ggtcctgacg cagagcgacg
     3181 aggaaaatgg gaacgccccc tgacagagcg tg
//
LOCUS       DASYCS010000347         3294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4591821,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000347 DASYCS010000000
VERSION     DASYCS010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4591821"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1926)
                     /locus_tag="VGM46_10870"
     CDS             complement(<1..1926)
                     /locus_tag="VGM46_10870"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEY4193135.1"
                     /translation="MGAYALLIGISRFDDPKLAKLNAPREDVEAFAGVLSDPARGGFD
                     NVATCLDQDLQTIRKQLAKLLLSGRNPNDMVLLYYSGHGIMTMGQRLFLATGQSSFDQ
                     PEADSLSALEMRDWLERSRAGKQVVILDCCHSGAFADGAKGVVQTVTGDTFASDNAEG
                     QYVLTATDALQFAYDANGTQRQDAAPVALSRFTGWLVDAIGKGLAAPDSDRITLDAVF
                     DYLSRRARAEAAGMTPSRFVKRNSGEMVIAHNPAARPPKLPAEIVAQLDSKDWQARRD
                     AVMQLGKLADQPAFRELAEQAVTDRVSLERDVDVRGAMLKFLRQIGADAAVLDAKFPP
                     PDPKPLPPGGGMKPGEGPDTNDPEPFWRRPKSLALAGGIALALIVMVGGAAIGIRSYQ
                     RLATVASYAQQQAADRAAAEQARMAEAAKEAEAAKTRADELLKQQQAEAAKAQAAAAG
                     KELAAAVLQAQRAAAAAKADQAKAQVPPAKDNSKELQGLAEALKQQKAQAALAQQQLA
                     SLEKTDEAARQIAGGQFKDCAECPAMITIPGGTMMMGSSGSESGRDRDEGPQHQVAIA
                     RNFAVGKFEVTFAEWDACVTGGGCNGYKPSDEGWGRGAHPVIFVSWNDAQTYIAWLRN
                     KTGKPYRLLSEAEWEYAA"
     gene            complement(1931..2305)
                     /locus_tag="VGM46_10875"
     CDS             complement(1931..2305)
                     /locus_tag="VGM46_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193136.1"
                     /translation="MDISVGVVAAGDTSHREVADLTDELRDAVGRLRGVAAVEPVETK
                     APGGAKGVGQILGAFLVSLPAGMVPAIFEVLKAIVSRPSQPPVTIELTAGSTKVSFDP
                     KQIKPADLAALVESLKPKVAGV"
     gene            2495..>3294
                     /locus_tag="VGM46_10880"
     CDS             2495..>3294
                     /locus_tag="VGM46_10880"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEY4193137.1"
                     /translation="MGAHALLVGISRFDDPKLAKLNSPQSDVAAFGGVLKDPARGGFD
                     SVEISIDRDLLDIRDQLSRLMDGRSADDMVLLYYSGHGIVAKGGRLYLATGQSSFDRP
                     QARSVATSEIRDLMEQSRAGRLVVILDCCHSGVFAEGAKGAEAPAMTDNTFGDGEGSE
                     GQYVLTATDALQYAYDGAGELREAAPGPTLSRFTRWLVDGIGKGEASPFDEQITLDAL
                     YQYLCRRAKTEQAGMTPQRFVKRGSGEMVIAKNPFGKALALPDDLIARL"
BASE COUNT          577 a         1053 c         1024 g          640 t
ORIGIN      
        1 ggcggcatat tcccattccg cttccgacag caggcggtag ggcttgccgg tcttgttgcg
       61 cagccatgcg atataggttt gcgcatcgtt ccagctcacg aagatgacgg ggtgagcgcc
      121 gcggccccac ccttcatcgg aaggcttgta accgttgcag ccgccgccgg tcacgcaagc
      181 gtcccattcg gcgaatgtga cctcgaattt gccgacggca aaattcctgg cgatggccac
      241 ctgatgctgc ggaccttcgt cacggtcgcg gcccgattcc gaacccgatg agcccatcat
      301 catcgtgccg cccggtatgg tgatcatcgc gggacattcg gcgcaatcct tgaattgccc
      361 tccggcaatc tgcctcgccg cttcgtccgt tttttccagg gatgcgagct gctgttgcgc
      421 cagggccgct tgcgccttct gctgtttcag cgcttccgcg aggccctgca attctttgct
      481 gttgtccttc gcgggtggaa cttgcgcttt ggcttggtcg gctttggccg ccgcggctgc
      541 gcgttgggcc tgcaagacgg cggcagcaag ctctttgccg gccgcggcgg cttgcgcttt
      601 ggcggcctcg gcttgctgct gtttcaacaa ctcatcggcg cgcgtcttgg ccgcctcggc
      661 ttccttggcg gcttcggcca tgcgtgcttg ctccgcggcg gcacggtcgg ccgcctgctg
      721 ctgggcatag ctcgcgacgg tagccagacg ctgataactc cggatgccga tcgccgcgcc
      781 cccgaccata acgataaggg caagcgcaat gccgcctgcc aaggcgagcg atttgggccg
      841 tctccagaac ggttcgggat cattcgtatc cggtccctcg cctggcttca ttccgccgcc
      901 gggcggaagc ggcttagggt ccgggggcgg gaatttcgca tcaaggacag ccgcatccgc
      961 gccgatctgc cgcaaaaatt tgagcatcgc gccgcgcacg tccacatcgc gttcgagact
     1021 cacgcgatcg gtcaccgcct gctcggcaag ttcgcgaaac gccggctgat ccgcaagctt
     1081 gccaagctgc atgacggcat cgcgccgcgc ctgccaatcc tttgaatcga gctgtgcgac
     1141 gatttccgcg ggcagtttcg gcggccgcgc ggcgggattg tgcgcgatca ccatctcgcc
     1201 cgaattgcgt ttgacaaagc ggctcggcgt cattccggcc gcttctgccc gcgcgcggcg
     1261 gctgagataa tcgaacaccg catccagcgt gatgcgatcg ctgtcgggtg cggccaggcc
     1321 tttgccgatg gcgtccacca gccagccggt gaagcgcgac aaagccaccg gcgcggcgtc
     1381 ctgacgctgc gtgccgtttg cgtcataggc gaattgcagc gcatcggttg ccgtcagcac
     1441 atattggccc tcggcattgt cgctggcgaa cgtatcgccg gtgacggttt gcaccacgcc
     1501 tttcgcgcca tcggcgaatg cgccgctgtg acagcaatcc aggatgacga cctgcttgcc
     1561 cgcgcgcgag cgctccagcc aatcgcgcat ttcgagcgcg gacaggctgt ccgcctcggg
     1621 ctggtcgaaa gacgattggc cggtggccag aaacagacgc tggcccatcg tcatgatgcc
     1681 gtggccggaa taatagagca gcaccatgtc gttgggattg cggccgctca gcaaaagctt
     1741 ggcaagctgt ttgcggatgg tttgaagatc ctgatcgaga caggtcgcga cattgtcgaa
     1801 accgccgcgc gcgggatcgc tgagcactcc ggcgaaagct tcgacgtcct cccgaggcgc
     1861 attcagcttg gcgagtttgg gatcgtcgaa ccggctgatg ccgatcaata gcgcataggc
     1921 gcccattgct tcagacgccc gcgaccttgg gtttgagcga ctcgacgagc gcggcaagat
     1981 cggccggctt tatctgcttt ggatcgaacg acactttcgt cgatcccgcc gtcaactcga
     2041 tcgtcaccgg aggctgcgat gggcgcgaca cgatcgcctt gagcacttcg aaaatcgccg
     2101 gaaccatgcc ggcgggcagg ctcaccagga atgcgcccaa gatctggccg accccttttg
     2161 cgccgccggg cgctttggtt tccaccggct cgacggccgc cacgccgcgc agacggccga
     2221 ccgcgtcgcg caactcgtcc gtcagatcgg ccacttcacg atgcgaagta tcgccggctg
     2281 cgacaacacc aaccgaaatg tccatgattc ccgcggcccc aaggaaaacc tcgaagatta
     2341 tacttaatcc tgctacaaac caaaagcttc ggcggaggaa tccggggaac ttaccgccta
     2401 ttttcagaca tgcgtcgcca gcgttcgagg gtcggcttct ttcgattgag aattgcctgt
     2461 cgatttggat gttttcccag tggaagagga ccctatgggt gcgcacgcgc tcctggtcgg
     2521 catcagccgg ttcgacgatc ccaaactcgc caagctgaat tcgccgcaga gcgatgtcgc
     2581 ggcatttggt ggtgtcctga aagacccggc gcgcggcggg ttcgacagcg tggaaatcag
     2641 catcgaccgg gaccttctgg acatacgcga tcagctcagc aggctgatgg acggacgcag
     2701 cgccgacgac atggtgctgc tttattattc aggccacggc atcgtcgcaa agggcgggcg
     2761 gctctatctg gcgactgggc aatcttcctt cgacaggccg caggcgcgca gcgttgcgac
     2821 cagcgaaatc cgcgatctca tggaacagtc gcgcgccgga cggctcgtgg tgatcctcga
     2881 ctgctgtcac agcggcgtat ttgccgaagg cgccaagggg gccgaagccc cggcgatgac
     2941 ggataatacg ttcggcgacg gcgagggctc cgagggacaa tatgtgctga ccgccaccga
     3001 cgcgctgcaa tacgcctatg acggggccgg tgaactgcgt gaagccgctc cgggtcccac
     3061 gctgtcgcgc ttcacccggt ggctggtgga cggtatcggc aagggcgagg cctcaccctt
     3121 cgacgagcag ataacgctcg atgccctgta tcagtacctg tgccgccgcg cgaaaacgga
     3181 acaggccggc atgacgccgc aacgtttcgt caaacgcggt tcgggcgaaa tggtgatcgc
     3241 caaaaatcct tttggcaaag cattggccct gcccgacgat ctcatcgcca ggct
//
LOCUS       DASYCS010000348         4293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3286464,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000348 DASYCS010000000
VERSION     DASYCS010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4293
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3286464"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..1080
                     /locus_tag="VGM46_10885"
     CDS             121..1080
                     /locus_tag="VGM46_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4193138.1"
                     /translation="MSSPILKLVAQRIALGIVLLLAVSVLIFAGTQVLPGDVAQSILG
                     QSATPEALANLRAELGLNDPAYIRYFHWLGGVLTGDLGTALSSGQDIATSIGGRLWNT
                     LFLAFCAAVISVPLAILLGLLAVRYRNGWVDKLISGLALASTSFPEFFIGYLLVYFFA
                     VKWQIFPGISTVYDGMPFLARLQAIALPAAALTLVVLAQMMRMTRAAILNVMQSAYVE
                     TAELKGLSAFSIIRKHALPNAVAPIINVVMLNLAYLIVGVVVVEVIFVYPGMGQYLVD
                     HVAKRDVPVVQAVGLIFAAVYITLNIVADIASIVANPRLRHPK"
     gene            1090..1914
                     /locus_tag="VGM46_10890"
     CDS             1090..1914
                     /locus_tag="VGM46_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4193139.1"
                     /translation="MLDIKRIPLPALVGIILTVLFLIAAIFAPWIAPHGNGEIVGDVW
                     QPMSAQFWLGTDNLGRDLLSRMIYGARITIFIAVLATALSFTLGASLGFCAAVLGGWF
                     DTLMSRFVDLLMAIPTLIFALVVLSVLPSTLVTLILVMGILDSTRVYRLSRAIAVDIE
                     VMDFVEAAKLRGEGMRWIIFREILPNALSPLISELGLRFIYAILFLSALSFLGLGVQP
                     PDADWGGMVKENKDGIVFGIGAALIPAAAIAMLAISVNLVADWVLNRTTSLKGGRG"
     gene            1914..3569
                     /locus_tag="VGM46_10895"
     CDS             1914..3569
                     /locus_tag="VGM46_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006325409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4193140.1"
                     /translation="MADQQRKSDVLLDIRNLRIEATVYPPGEPPKDIVIVHDVSLTLK
                     KGKVLGLIGESGAGKSTIGLSAMGYGRGGVRITGGEVILNGRDILKGGKQGLRKLRGH
                     EVCYVAQSAAAAFNPAHRLMEQVVEATLLHGRATRAEAEKRAIKLFRALSLPDPEHIG
                     ERFPHQVSGGQLQRVMTAMALCSEPDLIVFDEPTTALDVTTQIDVLAAIKVAIQETGV
                     AALYITHDLAVVAQVSDEIMVLRHGRLVEWGGTRQIIKEPRQEYTNALVSVHEIDHQE
                     KKPGAAPFLSVKNVTAAYGGGDVKVLKNVSVDIFPGQTLAVVGESGSGKSTLARAVTG
                     LLPPQQGTVTFDGRPLANRLADRPKEDLRQLQMIYQMADVAMNPRQTVGTIIGRPLEF
                     YFGMRGRERDARVAELLDKIEMGKGFIDRYPAELSGGQKQRVCIARSLAAKPKLIICD
                     EVTSALDPLVANGILKLLLELQAEENVAYLFITHDLATVKSIADSIAVMYRGEVVRYG
                     PKSEVLKPPFDAYTDLLLSSVPEMEIGWLEKAIKGRRMESAGN"
     gene            3629..>4293
                     /locus_tag="VGM46_10900"
     CDS             3629..>4293
                     /locus_tag="VGM46_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013891610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY4193141.1"
                     /translation="MPLSSKLLLIVLDGVPWRNWRRLMGNLEGWVQSGEARLWKMRSV
                     LPSTSACCYASIHTGVSPQVHGIRSNEDRFRVAQPDIFSEVSTAGGKTGAVTHSYWSE
                     FFRAYPFDLVEDMEFDEPGGSITHGRFHTMTGYNARNQMTPSDVDLFATLTMLTRRHG
                     IDYGILHTCTLDSMGHRFGHDCHEMDHAVYAMDAMLAAFLPGWIAAGYEVMVTADHGQ
                     TDRG"
BASE COUNT          732 a         1361 c         1345 g          855 t
ORIGIN      
        1 accagtatat cgacgcctcg ggcaagggcg tgcagggctg gatgaacaat cccgcccagg
       61 aaatgtgcaa cggctatgca ctgagcctgt gctggctgga ggcctgagcg ggcggcgcgc
      121 atgtcatctc caatcctgaa actcgtggcc cagcgcattg cgctgggcat cgtcctgctg
      181 ctggccgttt cggtcctcat cttcgcgggc acccaggtgc ttcccggaga tgtcgcgcaa
      241 tccattctcg gccagtcggc cacgcccgaa gcgctcgcca atctgcgcgc ggaactgggg
      301 ctgaacgatc cggcctatat tcgatatttc cactggctgg gcggcgtcct gaccggcgac
      361 ctcggcaccg cgttgtcgag cgggcaggat atcgccacat ccatcggcgg gcggctgtgg
      421 aatacgctgt ttctggcttt ctgcgccgcc gtcatctcgg tgccgctggc catccttctc
      481 ggcctgcttg ccgtgcgcta ccgcaatggc tgggtcgaca agctgatttc ggggctggcg
      541 cttgcctcga cctcgtttcc ggaattcttc atcggctacc tgctcgtcta tttcttcgcc
      601 gtgaagtggc aaatctttcc cggcatttcg accgtctatg acggcatgcc gttcctggcc
      661 cgcctgcaag ccatcgcctt gccggcagcg gcgctgacgc tggtggtgct ggcgcagatg
      721 atgcgcatga cgcgcgccgc catcctcaac gtcatgcagt cggcctatgt cgagactgcc
      781 gaactcaagg gcctgtcggc gttttcgatc atccgcaaac atgcgctgcc caatgcggtc
      841 gcgccgatca tcaatgtcgt gatgctcaat ctcgcctatc tgatcgtcgg cgtcgtcgtg
      901 gtcgaggtga tcttcgtcta tcccggcatg ggacaatatc tggtcgatca cgtcgccaag
      961 cgcgacgtgc cggtggtgca ggccgtcggg ctgatcttcg ccgccgtcta catcacgctc
     1021 aatatcgttg ccgatatcgc gtcgatcgtc gccaatccac gcctcagaca tccgaaatag
     1081 gggcgcgcca tgctcgatat aaaacgcatc ccccttccgg cgctcgtcgg catcatcctg
     1141 acggtgctgt tcctcatcgc ggcgatcttt gcgccatgga tcgcgccgca cggcaatggc
     1201 gagatcgtcg gcgatgtctg gcagccgatg tcggcgcaat tctggctcgg caccgataat
     1261 ctcggccgtg acctgttatc gcgcatgatc tatggcgcgc gcatcaccat cttcatcgcc
     1321 gtgctggcca ccgcgctgtc gttcacgctg ggcgcatcgc tcggcttctg cgcggcggtg
     1381 ctcggcggct ggttcgatac gctgatgtcg cgtttcgtcg atcttttgat ggcgatcccg
     1441 acacttatct tcgcgctggt ggtgctgtcg gtgctgccgt cgacgctggt gacgctgatc
     1501 ctggtgatgg gcatattgga ttccacgcgc gtctatcgcc tgtcgcgggc gatcgccgtc
     1561 gatatcgagg tcatggattt cgtcgaggcg gccaagctgc gcggcgaagg catgcgctgg
     1621 atcatcttcc gcgaaatcct gcccaatgcg ctgtcgcctc tgatttcgga actcggcctg
     1681 cgcttcatct atgccatact gttcctgtcg gcgctgtcct tcctcgggct tggcgtccag
     1741 ccgccggacg ccgactgggg cggcatggtc aaggaaaaca aggacggcat cgtgttcggc
     1801 atcggcgccg cgctcatccc ggcggccgcg atcgccatgc tggcgatttc cgtcaacctc
     1861 gttgccgatt gggtgctcaa ccgcacgaca agcctgaagg gagggcgcgg gtgatggccg
     1921 accagcagcg caagtccgac gttctgctcg acatccgcaa cctgcgcatc gaggcgacgg
     1981 tctatccgcc cggcgaaccg ccgaaagaca tcgtcatcgt ccacgatgtt tcgttgacgc
     2041 tcaaaaaggg caaggtgctt ggcctgatcg gcgaatccgg cgccggcaag tctaccatcg
     2101 gcctgtcggc catgggctat gggcgcggcg gcgtgcgcat caccgggggc gaggtcatcc
     2161 tcaacggccg cgacatattg aagggcggca aacagggcct gcgcaagctg cgtggccatg
     2221 aagtatgcta tgtcgcgcag tcggcggcag ccgccttcaa ccccgcccac cgcttgatgg
     2281 agcaggttgt ggaggcgacg ctgctgcatg ggcgggcgac ccgcgcggaa gccgaaaaac
     2341 gcgccatcaa gctgttcagg gcgctgagcc tgcccgaccc cgaacatatt ggcgagcgct
     2401 tcccgcacca ggtttcgggc ggccagttgc agcgtgtgat gacggcgatg gcgctgtgtt
     2461 cggagccaga cctgatcgtc ttcgacgagc cgaccaccgc gctcgacgtg accacgcaga
     2521 tcgacgttct ggcggcgata aaggttgcca tccaggaaac cggcgttgcc gcgctctaca
     2581 tcacgcacga tctggccgtc gtcgcccagg tgtcggacga gatcatggtg ctgcgccatg
     2641 gccggctggt cgaatggggc ggcacgcgcc agatcatcaa ggagccgcgg caggaatata
     2701 ccaacgcgct ggtgtccgtg catgagatcg accatcagga gaaaaagccg ggcgcagctc
     2761 ccttcctgtc ggtgaagaac gtgacggctg cctatggcgg cggcgacgtc aaggtgctga
     2821 agaacgtgtc ggtcgatata ttccccggcc agacgctggc ggtggttggc gaatccggct
     2881 ccggcaaatc gacgctggcg cgcgccgtca cgggcctttt accgccgcag caaggcaccg
     2941 tcaccttcga cggcaggccg ctggccaacc ggcttgccga ccggccgaag gaggatttgc
     3001 gccagttgca gatgatctac cagatggccg acgtggcgat gaacccgcgc cagaccgtcg
     3061 gcaccatcat cgggcggccg ctggagttct atttcggcat gcgcgggcgc gagcgcgatg
     3121 cgcgcgtggc cgaattgctc gacaagatcg agatgggaaa aggcttcatc gaccgctatc
     3181 cggccgaatt gtcaggcggg cagaagcagc gcgtgtgcat tgcgcgttcg ctggcggcca
     3241 agcccaagct tatcatctgc gacgaggtaa cttcggcgct cgacccgctg gtggcgaacg
     3301 gcattctcaa gctgttgctg gaattgcagg ccgaggaaaa cgtcgcctac ctgttcatca
     3361 cccacgatct ggcgacggtg aagtcgatcg ccgattccat cgcggtgatg tatcgcggcg
     3421 aggtcgtgcg ttatgggccg aaatcggaag tgctgaagcc gccattcgat gcctataccg
     3481 accttctcct gtcttccgtt cctgaaatgg aaatcggctg gctggaaaag gcgatcaagg
     3541 gacggcgcat ggaaagtgcc gggaactaga ccggttattc gcccgcaggg agagtaacga
     3601 ccggaccgca tcgcccagga actttcgcat gccgctttcc tccaagcttc tcctcatcgt
     3661 cctcgacggc gttccgtggc ggaactggcg caggctgatg ggcaatctcg aaggttgggt
     3721 gcagtccggc gaggcgcgtc tatggaaaat gcgttccgtg ctgccctcga cgtcggcctg
     3781 ctgctatgcc tcgatccata ccggcgtgtc gccgcaggtg catggcatcc gctccaacga
     3841 agaccggttt cgcgtcgcgc agcccgacat tttctccgaa gtcagcacgg cgggcggcaa
     3901 gaccggcgcg gtgacgcatt cctactggtc ggaattcttc cgtgcctatc cgttcgactt
     3961 ggttgaggac atggagttcg acgagccagg cgggtcgatc acacatggcc gcttccacac
     4021 catgaccggc tacaatgccc gcaaccagat gacgcccagc gatgtcgacc ttttcgcgac
     4081 cctgaccatg ctgaccaggc gccacggcat cgactacggc atcctgcaca cctgcacgct
     4141 ggactcgatg ggccatcggt tcggccacga ctgccacgag atggaccatg ccgtctatgc
     4201 catggacgcc atgttggccg ccttcctgcc gggctggatc gcggccggat acgaggtcat
     4261 ggtcaccgcc gaccatggcc agaccgaccg tgg
//
LOCUS       DASYCS010000349         2760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_677048,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000349 DASYCS010000000
VERSION     DASYCS010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677048"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..155
                     /locus_tag="VGM46_10905"
     CDS             <1..155
                     /locus_tag="VGM46_10905"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006331545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEY4193142.1"
                     /translation="AVNVTLGLPFIRTSPDHGTAFDIAGKGIARPDSLIAALKLARRL
                     ADNDTP"
     gene            152..982
                     /gene="rsmA"
                     /locus_tag="VGM46_10910"
     CDS             152..982
                     /gene="rsmA"
                     /locus_tag="VGM46_10910"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010915178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEY4193143.1"
                     /translation="MSIDGLPPLREVIESHGLQAKKALGQNFLLDLNLTGKIARSAGD
                     LSQGVVIEVGPGPGGLTRALLMHGARRVIAIERDERCLAALAEISEHYPGRLEVISGD
                     AMKTDFAALADGEPVKIVANLPYNIGTELLVRWLTVENWPPFYTSMTLMFQREVAERI
                     VAKPGSDAYGRLGVLAGWRAEATIAFDVPAQAFTPPPKVTSSVVHLEPRALPLTAEVK
                     RLARVTEAAFGQRRKMLRQSVKSLGGEELLNRVGIDPTRRAETLSVEEFVQLANAGRF
                     "
     gene            complement(986..2458)
                     /locus_tag="VGM46_10915"
     CDS             complement(986..2458)
                     /locus_tag="VGM46_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HEY4193144.1"
                     /translation="MAKALMLQGTGSDVGKTVLVAGLCRAARNRGLKVRPFKPQNMSN
                     NAAVADIPGEAGQSGEIGRGQWLQALACGVQPTVHMNPVLLKPQTDIGSQIIVQGKVF
                     GEAKARDYQGLKPRLMEAVLHSWTQVGEGADLVIVEGAGSPAEINLRAGDIANMGFAT
                     RANVPVVLVGDIDRGGVIASVAGTHLILPKGDRHMVAGYLINKFRGDVSLFDAGIAAI
                     GEFTGWRCFGVVPWLKAAARLPSEDSVVLERLASGGGRALKVAVPMLARIANFDDLDP
                     LKAEPQVEVVFVPPGQSLPEDAGLVVIPGSKSTIGDLLYLRANGWDRDLASHVERGGH
                     VIGICGGFQMLGKIVRDPLGIEGSVTETPGLGLLDVETVMEPEKTVRNVAARSVPFDL
                     PLEGYEIHLGRTTGPDMMRPATTIDGVADGAVSRDGRVFGTYMHGLFSADAFRGKFLE
                     SLGVKRGGIDYLAQVERALDDIAAELEAHLDCDALFSLAR"
     gene            complement(2458..>2760)
                     /locus_tag="VGM46_10920"
     CDS             complement(2458..>2760)
                     /locus_tag="VGM46_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019858666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047869 - dimethylpropiothetin
                     dethiomethylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfonioproprionate lyase family
                     protein"
                     /protein_id="HEY4193145.1"
                     /translation="LFVNETVAGGFLILGPEIVYPDHHHVAEEIYVPLTGGTEWRMGE
                     GGFVARRAGEVIHHASNVNHAMRTGSEPLLALYIWRGGPLAQKSTVTGTVAQQGRA"
BASE COUNT          507 a          967 c          827 g          459 t
ORIGIN      
        1 aggcggtgaa cgtcacgctt ggcctgccct ttatccgcac ctcgccggac cacggcaccg
       61 ccttcgacat tgccggcaag ggcatcgccc gccctgacag tctgatcgcg gcgctgaagc
      121 tcgcccgccg ccttgccgat aacgacaccc catgagcatc gacgggctgc cgccgctgcg
      181 cgaggtgatc gagagccatg gccttcaggc aaagaaggca ctcggccaga atttcctgct
      241 cgacctcaac ctgaccggaa agatcgcgcg cagcgccggc gatctctcgc aaggcgtggt
      301 catcgaggtc ggccccggcc ccggcggcct gacccgtgcg cttttgatgc acggcgcgcg
      361 ccgtgtcatt gcaatcgagc gcgacgaacg ctgcctggcc gccctggccg aaatctcgga
      421 acactatcca ggccggcttg aagtgatttc cggcgacgcc atgaagaccg acttcgcggc
      481 gctggccgac ggcgaaccgg taaagatcgt cgccaacctg ccctacaata tcggcaccga
      541 acttttggtg cgctggctga cggttgaaaa ctggccgccc ttctatacct cgatgacgct
      601 gatgtttcag cgcgaggtgg ccgagcgtat cgtggcgaaa cccggcagcg atgcctatgg
      661 ccgccttggc gtgctggcag gctggcgcgc ggaagcaacc atcgccttcg acgtgccggc
      721 gcaggctttc accccgccgc ccaaggtaac gtcttcggtg gtgcatctgg agccgcgcgc
      781 tttaccgctg accgccgagg taaagcgcct ggcccgcgtc accgaggcgg ccttcggcca
      841 gcgccgcaag atgctgcggc aaagcgtgaa gagccttggc ggcgaagagc ttctcaaccg
      901 ggtcggcatc gatcccaccc gccgcgccga aacgctgagc gtcgaggaat tcgttcaact
      961 ggccaacgcc ggacgcttct gatcgtcagc gcgccagcga aaacagcgca tcgcaatcga
     1021 gatgcgcttc aagttccgcc gcaatgtcgt ccagcgcccg ctcgacctgc gccaggtagt
     1081 cgatgccgcc tctcttgacg ccaaggcttt caaggaactt gccgcgaaag gcgtcggcgg
     1141 aaaacaatcc gtgcatatag gtgccgaaca ccctgccgtc gcgcgacacg gcgccatcgg
     1201 ccacgccgtc gatggtggtg gcgggacgca tcatgtccgg cccggtcgtg cggccgagat
     1261 gaatttcgta accctccagc ggcaggtcga acggcaccga gcgcgccgcg acattgcgca
     1321 ccgtcttttc cggctccatc acggtttcca cgtcgagcag tccaaggccg ggcgtttcgg
     1381 tcacgctgcc ctcgatgccc agcggatcgc gcacaatctt gcccagcatc tggaagccgc
     1441 cgcaaatacc aatgacatgc ccgccccgtt cgacatggct ggcaaggtca cggtcccacc
     1501 cattggcacg cagatagaga aggtcaccga tggtcgactt ggaaccgggg ataaccacca
     1561 gcccggcatc ttcaggcaag gattggccgg gcggcacgaa cacgacctcc acctgaggct
     1621 ccgccttcaa gggatcgagg tcgtcgaaat tggcgatgcg cgccaacatc ggcaccgcca
     1681 ctttcagcgc ccgcccgccc ccggatgcca accgttccag caccaccgaa tcctccgaag
     1741 gcagccgcgc cgccgccttt agccacggca cgacgccgaa gcagcgccag ccggtaaatt
     1801 cgccgattgc cgcgatcccc gcatcgaaca gggaaacgtc gccgcgaaac ttgttgatga
     1861 gatagcccgc caccatgtgc cggtcgcctt tcggcaagat caggtgcgtg ccggcgacgg
     1921 aggcgataac cccgccacgg tcaatgtcgc ccaccagcac tacaggcaca ttcgcgcgcg
     1981 tcgcaaagcc catattggcg atatcgccgg ctcggaggtt gatttccgcc ggcgagccgg
     2041 cgccctcgac gatgacgagg tccgcaccct cgcccacctg cgtccacgaa tgtagcaccg
     2101 cttccatcaa ccggggcttc aggccctgat aatcacgcgc cttcgcctcg ccgaacacct
     2161 tgccctgcac aatgatctgc gagccgatgt cggtctgcgg cttcagcagc accgggttca
     2221 tgtgaaccgt tggctgcacg ccgcaggcca gcgcctgtag ccattgcccg cgcccgatct
     2281 cgccggattg cccggcttcg cccggaatat cggcgacggc ggcattgttg gacatgttct
     2341 gcggcttgaa tggccgcacc ttgaggcccc gattcctcgc cgcgcggcaa agcccggcaa
     2401 ccagcaccgt cttgccgaca tccgagccgg tgccttgcag catgagcgcc ttggccatca
     2461 ggccctgccc tgctgcgcca cggtgccggt aacggtcgat ttctgcgcca gcggcccgcc
     2521 gcgccagatg tagagcgcca gcagcggctc cgaacctgtc cgcatcgcat ggttgacgtt
     2581 ggaggcgtga tggatgacct cgccggccct tcgcgcaaca aagccgccct cgcccatgcg
     2641 ccattccgtg ccaccggtga gtggaacgta gatttcctcg gcgacatggt ggtggtcggg
     2701 ataaacgatt tccggcccaa gaatgagaaa cccgccggcc acggtttcgt tgacgaaaag
//
LOCUS       DASYCS010000350         7097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_531922,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000350 DASYCS010000000
VERSION     DASYCS010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 7097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7097
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531922"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1876
                     /locus_tag="VGM46_10925"
     CDS             <1..1876
                     /locus_tag="VGM46_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY4193146.1"
                     /translation="MTRIAAFSPLLFWPGIIGDFMKYLPITLIVTLAASMLYALVFAP
                     TLGAMFAKAPKHHEDDHRDGWYMAVVKQAVRFPKTVLVLTVVLLVGVQFSYSKFGAGV
                     EFFPSVEPDYGLLYVHARGNLSLAEMDIATREAEKRLLGWPGVKSVYTRVGKTQGGGA
                     DIPEDVVGVIQYEFVDWRERKSANQILDDLRKVMAGIPGIDVEVRVPEAGPPTGKPIQ
                     VRLSAVDPAGLNDKAREVAARIGKIPGVIDVSDGLPPPGVDWALNVDRAKAAQYGISP
                     SSVGTVVQLVTNGLKLSEYRPAGADDAVDIRLRLPEDQRTLSTLDELRVQTSQGAVPI
                     SNFVDRKPEQRVGTLNRIDGARTVVVQANVAADKQVAAVQQEVTKAISGMDLGSGVRW
                     KLAGSNEDSAEASAFLSKAFGAAIFLIFIVLLAQFNKFTSVWLVLSCVVMATIGVFLG
                     LLITGETFGIVMSGIGVIALAGVVVNNNIVLIDTYDRLREEGWDKTEAVLQTCRERAR
                     PVVLTAVSAILGVLPIAFGLGLEIFHHETTYNAPSTQWWISLSSAIVFGLSFATVLTL
                     VVTPSMLMVFTRAKVQPGQGRSWWSRLFGHGRKGEAVAGETPVNKGAEQGLALPKAAG
                     "
     gene            1980..2138
                     /locus_tag="VGM46_10930"
     CDS             1980..2138
                     /locus_tag="VGM46_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HEY4193147.1"
                     /translation="MTLTTILVLVLILVLIGAIPGWPYSRGWGYIPSGIVGAVLLIVL
                     VVILIGRM"
     gene            complement(2154..3338)
                     /locus_tag="VGM46_10935"
     CDS             complement(2154..3338)
                     /locus_tag="VGM46_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018430064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4193148.1"
                     /translation="MDAINAANTARRGRWAVATMFLANGFLTGSWAPQIPVLLTRLDI
                     SKATLGLLILLFGVGAVAAMTWCGHLISRHGSRNVVRWFGVCASFGLLLVALAPSVPL
                     AAVAMILFGGVVGGMDVAMNANAVIVERKLARAIMSSSHGFWSLGGFAGGALGGFAIQ
                     HFGHVAHAAAATLIAFVAIVSAMGHLVGEDEPATHGRHASFSLPRQPAIYLIGIMALF
                     AMIPEGAVLDWSALYLRQEMGADLATAGLAFAAFSGVMALMRFLGDGVRNRFGAVKTL
                     RISAILAALGMLVAGLSPSPWLAIAAFAFCGFGIANMVPIAFSAAGNQTGMPSGTGMS
                     VVSTMGYCGILVAPSAIGFIAGHTGFGPVFIALSGLLVVVFLMAGLAKRADFSAAPAA
                     AE"
     gene            complement(3461..3769)
                     /locus_tag="VGM46_10940"
     CDS             complement(3461..3769)
                     /locus_tag="VGM46_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006327831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1244 domain-containing protein"
                     /protein_id="HEY4193149.1"
                     /translation="MTELSDTQKRDLEAAAFRRLVGHLRERTDVQNIDLMNLAGFCRN
                     CLSNWYREAAEQEGVALSKDESREIVYGMPYAEWQALNQTEASDAKLAAFEARRPKDH
                     "
     gene            complement(3782..4579)
                     /locus_tag="VGM46_10945"
     CDS             complement(3782..4579)
                     /locus_tag="VGM46_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HEY4193150.1"
                     /translation="MTRSTVFAPFDLIEGDRSRGIVLVADHARRDLPDEYGDLGLPAS
                     EFDRHIAYDIGVEAVTRELAAQLGVPAVIATFSRLLIDPNRGEDDPTLIRQLYDGTVV
                     PANYPIDAKQREKRLDGFYRPYHDAVGAMIASVAQATGAAPLVFSVHSFTPVMHGHVR
                     PWHAGVLWDSDDRVARPLMDLLATDANLVVGDNEPYDGALRGDTMYKHAVVNGFAHAL
                     LEIRQDLIATGKDAIAWAMRLAPIIEEINRRPNIHQVKQFGSRTGPF"
     gene            4871..5314
                     /locus_tag="VGM46_10950"
     CDS             4871..5314
                     /locus_tag="VGM46_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007008280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1036 domain-containing protein"
                     /protein_id="HEY4193151.1"
                     /translation="MPLAILAGLIVTVAASPAHADFRVCNATQNLVGVGIGYRAKAGW
                     ITEGWWHIEGSSCKTLIEGPLSSRFYYLYAEDAERGGRWDGPINMCVAEKEFKIAGVS
                     DCVARGFQRAGFQEYDTGEQSSWMVQLTDQPATDGDGAAPESKSP"
     gene            5311..6744
                     /gene="pyk"
                     /locus_tag="VGM46_10955"
     CDS             5311..6744
                     /gene="pyk"
                     /locus_tag="VGM46_10955"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEY4193152.1"
                     /translation="MRRNRKVKILATIGPASSSEEMLKKLFEAGADVFRINMSHTDHE
                     LMRTLIGRIREVETSVGRPIGILADLQGPKLRVGKFANGKETLSVGQTFTLDDSQEPG
                     DASRVYLPHPEILSSVKAGHRLLIDDGKLELRAVSSNGKQIVCTVVAGTGISDKKGVS
                     LPDTELPVGALTEKDRSDLDAALAAGVDWIALSFIQRPEDLAEARKIARGRALIMSKI
                     EKPQAVARLAEIIELSDALMVARGDLGVEVPLEAVPGIQKQITRAGRRAGKPVVVATQ
                     MLESMISAPVPTRAEVSDVATAVFEGADAIMLSAESAAGAYPVEAVATMNRIAVKVEQ
                     DPTYAGIINAQRSEPEATGADAISLAAREIAETLKLAAIITYTASGTTGLRAARERPQ
                     VPIIALSPIVNTARRLSLLWGTHCVVSPDASDLDDMVNRASRIALEEGFGQRGDRVII
                     TAGVPLRTPGSTNMLRIAYVGSEEGGR"
     gene            complement(6792..>7097)
                     /locus_tag="VGM46_10960"
     CDS             complement(6792..>7097)
                     /locus_tag="VGM46_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193153.1"
                     /translation="KVVLAKIHSVGLVRDLAGAELVWVKNLGHKPDWIAADLVVAAIQ
                     KVAGQKVAGQKVSGNQPDLQSLAKTVEARIAKDAYGTGRCADIAEPDAEPDAELAPT"
BASE COUNT         1308 a         2162 c         2378 g         1249 t
ORIGIN      
        1 gatgacgcgc atcgccgcct tctcgccgct tttgttctgg ccgggcatca tcggcgattt
       61 catgaaatat ctgccgatca cgctgatcgt gacgctggcg gcgtcgatgc tctatgcgct
      121 ggtgtttgcg ccgacgctgg gggcgatgtt cgccaaggcg cccaagcacc atgaggacga
      181 ccaccgcgac ggctggtaca tggctgtcgt caagcaggcg gtgcggtttc ccaagacggt
      241 gctggtgctg acggtcgtgc tgctggtcgg cgtgcagttt tcatattcga agttcggcgc
      301 cggcgtggag ttcttcccga gcgtcgagcc ggattacggc ctgctctatg tgcatgctcg
      361 cggcaatctt tcgctggctg aaatggatat tgcgaccagg gaggccgaaa agcgcctact
      421 tggctggccg ggcgtcaagt ccgtctatac gcgcgtcggc aagacgcaag gcggcggcgc
      481 ggatattccc gaggacgtgg tcggcgtcat ccagtacgag ttcgtcgact ggcgcgagcg
      541 caagtccgcc aaccagatac tggacgatct gcgcaaggtg atggccggca ttccgggcat
      601 cgatgtcgag gtgcgcgtgc cggaagccgg tccgccgacc ggcaagccga tccaggtgcg
      661 gctgtcggct gtcgatccgg ccgggctgaa cgacaaggcc cgcgaagtgg cggcgcgcat
      721 cggcaagata ccgggcgtga tcgacgtttc cgacggcctg ccgccgcccg gcgtcgactg
      781 ggcgctcaat gtcgaccggg ccaaggcggc gcaatatggc atcagcccga gttccgtcgg
      841 caccgtggtg cagctggtga cgaacggcct gaagctgtcc gaataccggc ccgccggcgc
      901 ggatgacgct gtcgatatcc ggctgcgcct gccggaagac cagcgcacgc tgtcaacgct
      961 tgacgaattg cgggtgcaga ccagccaggg cgcggtgccg atttccaact tcgtcgaccg
     1021 caagcccgag cagcgcgtcg gcacgctcaa ccgcatcgat ggcgcgcgca cggtcgtggt
     1081 gcaggccaac gttgcggcgg acaagcaggt cgccgccgtc cagcaggagg tgaccaaggc
     1141 gatctccggg atggacctcg gcagcggcgt gcgctggaag cttgccggct ccaacgaaga
     1201 cagcgcggag gccagcgcct tcctgtcgaa ggcgttcggc gcggcgatct tcctgatctt
     1261 catcgtgctt ctggcgcagt tcaacaagtt caccagcgtc tggctggtgc tgtcctgcgt
     1321 ggtcatggcg acgatcggcg tgtttctcgg cctgctgatc accggcgaga cgttcggcat
     1381 cgtcatgtcg ggcatcggcg tcattgcgct ggccggcgtg gtggtgaaca acaacatcgt
     1441 cttgatagat acctatgacc ggctgcgcga agaaggctgg gacaagacgg aagccgtgct
     1501 gcaaacctgc cgcgagcggg cgcgcccggt ggtgttgacg gcggtttcgg ccattttggg
     1561 cgtgctgccg attgcgttcg gcctcggcct ggaaatcttc caccacgaga cgacctacaa
     1621 cgcgccatcg acccagtggt ggatttcgct gtcctcggca atcgtcttcg ggctatcctt
     1681 cgcgaccgtg ctgaccctgg tggtgacgcc gtcgatgctg atggtgttta cgcgagccaa
     1741 ggtacagccc ggccagggcc gcagctggtg gagcaggctg ttcgggcacg gcagaaaggg
     1801 cgaggcggtt gccggcgaaa cccccgttaa taagggcgct gaacaggggc ttgccttgcc
     1861 aaaggctgct ggataggcgg agcaggccta gctccagacc ctttgggggt cgtccggaac
     1921 gattgcgcgg cctttcgcgt tttcgggctg gccggcaaag ttcggggcag ggatttgcga
     1981 tgacgcttac caccattctc gtgctggtct tgatccttgt tcttatcggc gcaattccag
     2041 gctggcccta ttcgcgcggc tggggttaca tcccttcggg aatagtcggc gcggtcttgc
     2101 tcatcgtcct ggtcgttatc ctgatcgggc ggatgtgacg gcggccggac agcctattcg
     2161 gcggctgcgg gagcggcgct gaaatccgcc cgcttggcca gtccggccat caggaagacc
     2221 acgaccagaa gccctgaaag cgcgatgaac accggaccga aaccggtatg cccggcgatg
     2281 aagccaatgg ccgaaggtgc taccaatatg ccgcaatagc ccatcgtgct gacgacgctc
     2341 atgccggtgc cggacggcat gccggtctgg ttgccggcgg cggaaaaggc gatgggcacc
     2401 atgttggcga tgccaaaccc gcaaaaggca aaggcggcga tcgccagcca cggcgagggc
     2461 gacaggcccg cgaccagcat gccgagcgcg gcgagaattg cggatatgcg taaggttttt
     2521 accgcgccga agcggttgcg cacgccgtcg cccaaaaagc gcatcagcgc catgacgccg
     2581 gaaaaggccg cgaaggcgag gccggcggtg gcgagatcgg cgcccatttc ctggcgcagg
     2641 taaagcgccg accagtccag caccgcgcct tccgggatca tcgcgaataa cgccatgatg
     2701 ccgatcaggt agatggccgg ctgcctgggt agcgagaagc tggcatggcg gccatgcgtg
     2761 gcgggctcgt cctcgccgac tagatggccc attgccgaga cgattgccac gaaggcgatg
     2821 agggtggccg cagcggcgtg ggcgacatgg ccgaaatgct ggatggcgaa gccgccgagc
     2881 gcgccgccgg cgaagccgcc caggctccag aagccgtgcg acgacgacat gatggcgcga
     2941 gcgagctttc gttcgacaat gaccgcattg gcgttcatgg cgacatccat gccgccgacc
     3001 acgccgccga agaggatcat ggcgaccgcc gccagcggaa cgctgggcgc cagcgccacc
     3061 agcaacaggc cgaaactggc gcagacgccg aaccagcgca cgacattgcg cgaaccatgg
     3121 cgcgaaatca ggtggccgca ccatgtcatg gccgccaccg cgccgacgcc gaacagcagg
     3181 ataagcaggc caagtgtcgc cttgctgatg tcgagacggg taagcaggac gggaatttgc
     3241 ggcgcccagc ttcccgtcag gaagccgttg gcaaggaaca tcgtcgctac cgcccagcgg
     3301 ccccggcggg cggtattcgc ggcattgatg gcgtccatgg cgactgtcca gaaagggggc
     3361 cgaagtcgcg ctggaaccta aatcggttca ggtcgaagtc aagcattggt cggtaaagct
     3421 ttgcggtggc aagtaaagcc tggcttcggc tggaaaagga tcagtgatcc ttggggcggc
     3481 gcgcctcgaa ggcagccagc ttggcgtccg acgcctcggt ctggttgagc gcctgccatt
     3541 cggcataggg catgccatag acgatttccc gcgactcgtc cttggagagc gcgacgcctt
     3601 cctgctcggc ggcttcgcga taccagttgg acaggcagtt gcggcagaag ccggcgaggt
     3661 tcatcaggtc gatgttctgc acgtcggtgc gttcgcgcag atgcccgaca agccggcgaa
     3721 aggcggcggc ctcaagatcg cgcttttgcg tatcgctcaa ttcagtcatc gccatctccc
     3781 atcagaacgg tccggttctg gacccgaatt gctttacctg atgtatattg ggccggcggt
     3841 tgatttcttc aatgatcggc gccagccgca tcgcccaggc gatggcgtct tttccggttg
     3901 cgatgaggtc ctggcggatt tccagcagcg catgggcgaa gccgttgacg acggcgtgct
     3961 tgtacatggt gtcgccgcgc agcgcgccgt catagggttc gttgtcgccg accaccaggt
     4021 tggcgtcggt ggcgagcagg tccatcaagg gccgcgccac gcggtcgtcg ctgtcccaca
     4081 gcacaccggc atgccacggg cggacatggc catgcatgac aggcgtgaac gagtgaaccg
     4141 aaaagacgag gggagccgcg cccgtggcct gcgccaccga agcgatcatg gcgccgacag
     4201 cgtcgtgata aggccggtaa aaaccgtcca gccgcttttc gcgctgtttt gcatctatcg
     4261 ggtaatttgc cggcaccacc gtgccgtcat agagctggcg gataagcgtc gggtcgtcct
     4321 cgccgcgatt gggatcgatc agcaggcgcg aaaaggtggc gatcaccgcc ggcacgccaa
     4381 gctgcgcggc aagctcgcgc gtaaccgcct cgacgccgat gtcataggcg atgtgacggt
     4441 cgaattcgga agccggcagg ccaaggtcgc catattcatc cggcaggtcg cggcgggcat
     4501 gatcggcaac cagcacgatg ccccggctgc ggtcgccttc aataaggtcg aagggcgcga
     4561 aaactgtgga tcgggtcatg gctgagaaaa aggatgccta tgtggtccag acaggggtga
     4621 tagcgtcatt ccacgaacag ggaacgcgcg caatgccgtt gtttggacaa ggtttttgca
     4681 gaaatctggc tgagcgtcgc ctgaaaccgc acctgtcttc atctaaatcg attggattta
     4741 ggcaaatcag cgcgttgaca tgcgcttgga cttttccgaa aaggggcttg ggagccagat
     4801 gatcgcgtcg tgtttgaggg tttcgaggat gattctcacc ggcaggaagc gcctatgcgc
     4861 gacctttgcc atgccgcttg caatccttgc gggcctgatc gtgacggtgg cggcatcgcc
     4921 ggcgcacgcc gatttccgcg tctgcaatgc gacgcagaac ctggtcgggg tcggcatcgg
     4981 ctaccgcgcc aaggcgggct ggatcaccga aggctggtgg cacatcgaag gttcgagctg
     5041 caagacgctg atcgaggggc cgctgtcatc caggttttac tatctctatg cagaagatgc
     5101 cgaacgcggc ggccgctggg atggacccat caacatgtgc gtggctgaaa aagagttcaa
     5161 gatcgccggc gtcagtgatt gcgtggcgcg cgggttccag cgagccggtt tccaggaata
     5221 cgacacgggc gagcagtcaa gctggatggt ccagctgacg gatcagcctg caacggacgg
     5281 cgatggcgcc gcccccgaaa gcaagagtcc atgagacgca accgcaaggt aaagatactg
     5341 gccacgatcg gcccggcttc ttcgtcggaa gagatgctga aaaagctgtt cgaggccggc
     5401 gcggacgtct tccgcatcaa tatgagccat accgaccatg agttgatgcg cacgcttatc
     5461 ggccgtatcc gcgaagtgga gacaagcgtc ggcaggccga tcggcatcct cgccgatttg
     5521 caggggccga agctccgggt cggcaaattc gccaacggca aggaaacctt aagcgtcggc
     5581 cagaccttca cgctggacga cagccaggaa cccggcgacg cctcgcgcgt ctatcttccg
     5641 caccctgaaa tcctgagttc cgtgaaggcg ggccaccgcc tgctgatcga tgacggcaag
     5701 ctggaactcc gcgccgtcag cagcaatggc aagcagatcg tctgcacggt cgttgccggc
     5761 accggcattt ccgacaagaa gggcgtcagc ctgcccgaca ccgaattgcc ggtcggtgcg
     5821 ctgaccgaga aggatcgcag cgatctcgac gccgcgctgg ccgccggcgt cgactggatc
     5881 gcgctttcct tcatccagcg cccggaggat ctggccgaag cacgcaagat cgcgcgtggc
     5941 cgcgcgctca tcatgtcgaa gatcgagaag ccgcaggccg tggcgcggct ggccgaaatc
     6001 atcgaattgt ccgacgcgct gatggtggcg cggggcgatc tcggcgtgga agtgccgctg
     6061 gaagcggtgc ccggcataca aaagcagatc acccgcgccg gccggcgcgc cggcaagccg
     6121 gtggtcgttg ccacgcagat gctggaatcg atgatttcgg cgcccgtgcc gacacgcgcc
     6181 gaagtatcgg atgtcgcaac cgccgtgttc gaaggcgccg acgccatcat gctgtcggcg
     6241 gaatcggcgg cgggcgccta tccggtcgag gcggtggcga ccatgaaccg catcgccgtc
     6301 aaggtggagc aggacccaac ctatgccggc atcatcaacg cccagcgttc cgaaccggaa
     6361 gcgaccggcg ccgacgccat ctcgcttgcc gcgcgcgaaa tcgccgagac gctgaagctg
     6421 gcggccatca tcacctatac cgcgtcgggc accaccggcc tgcgggcggc gcgagaacgt
     6481 ccgcaagtgc cgatcatcgc gctgtcgccc atcgtcaaca cggcgcggcg tctttcgctg
     6541 ctgtggggca cgcattgcgt ggtttcgccg gatgcgagcg acctcgacga catggtcaac
     6601 cgcgcctcgc ggatcgcgct ggaagaaggc ttcggccagc gcggcgaccg cgtcatcatc
     6661 accgcgggcg tgccgctgcg caccccaggc tcgaccaaca tgctgcgcat cgcctatgtc
     6721 ggatcggaag agggcggcag gtaaaggctt ttcgctggcg cgaaatccgc gccggctgtc
     6781 tatccggccg gttatgtcgg cgcaagttcg gcatcaggct cggcatcagg ctcggcaatg
     6841 tcggcgcatc ggcccgtgcc ataggcatcc ttggcgatcc gcgcctcgac ggtttttgcc
     6901 agcgattgca ggtctggctg gtttccagac actttctggc cagcgacttt ctggccagcg
     6961 actttctgga ttgccgcgac aaccaggtcg gcggcgatcc agtccggctt gtggccgagg
     7021 tttttcaccc agaccagttc ggcgccggca aggtcgcgga cgagaccgac cgagtggatt
     7081 ttggcgagca ccacctt
//
LOCUS       DASYCS010000351         3170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1255264,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000351 DASYCS010000000
VERSION     DASYCS010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1255264"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1867)
                     /locus_tag="VGM46_10965"
     CDS             complement(<1..1867)
                     /locus_tag="VGM46_10965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011424697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY4193154.1"
                     /translation="MNMDAGHRKPKLVFFQWDHQPNADAASYLLLHMQQHVKCLATHF
                     EVVVVNQDCDYAAICDRHQPDLTLFESGYRSHGSRRTIITNTAANAKVPKLGLHNADP
                     WCDRRAGFLSDMERWGIETYFSISTMMPDYTPEIAASLFIWPNFIDPELYHDYGQHKT
                     IPVTLTGQIYGLYPWRQQIFPLIRERYPCLVSPQHDYASKRSAGMLAGEAYARALNAS
                     FIVPTCGTQGGEVVRKHFEIPGAKACLLTERTPALEAAGFLDMENCVFASHHDVTDRI
                     DHLFAHPDEMQRITQAGHELVHARHTLKHRPQIHQWYMLNRDLQPGEKIIQSGPFGDL
                     EKVTHAAGRDSIHISGSARDRALLARGDIFLKQGRLAEARQCYAQCLDYVSYLPEAQF
                     RLALCALHEGNADQARESLARLIEITTLTYGAADPDPVEWAYFLLSMICTGELGQARR
                     IRDFYPGLGHEELVRARHVLDWLADGVSQDAQPDHLPKHRKSIHRLESRPDGEWLAWF
                     ATILDRCRQPELAARLRDLSAASLSGKAQSRRTGVGAATGWRNVAYAGADRLIAISGL
                     GALRPNVPPMAEFRYFERVARNFGRNIFRGRLRPLGLALSRLATAMREVGPAERIR"
     gene            complement(1864..2913)
                     /locus_tag="VGM46_10970"
     CDS             complement(1864..2913)
                     /locus_tag="VGM46_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003576819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HEY4193155.1"
                     /translation="MHGRKRILVTGGAGFLGSFLCERLLREDNDVLCVDNYYTGSRDT
                     VLHLLDDPRFEILRHDVTFPLYVEVDEIYNLACPASPVHYQFDPVQTVKTNVHGAINL
                     LGLAKRTKAKIFQASTSEVYGDPAVHPQTEAYRGSVNPIGPRACYDEGKRCAETLFFD
                     YHRQYGVNIRVARIFNTYGPRMHPDDGRVVSNFIVQALRNEPITLFGDGRQTRSFCYV
                     EDLIDGFIRLMAAPDSITGPVNLGNPGEFQMRELAEMIIELTGSASPIVFRPLPADDP
                     TRRKPDISHARHALGWQPTVPLRQGLEKTIAYFEAKLSGIATPPFEAAKAMSHPRVPL
                     TAAGLPTREGARLPT"
     gene            complement(2952..>3170)
                     /locus_tag="VGM46_10975"
     CDS             complement(2952..>3170)
                     /locus_tag="VGM46_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018071883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEY4193156.1"
                     /translation="YHLLAMNAEFRHKRRSVTPGLTGLWQISERSNADLELQRQLDEF
                     YIDNRSLWFDWHILLSTIPAVFRRNGAY"
BASE COUNT          590 a          912 c         1050 g          618 t
ORIGIN      
        1 ccctgatccg ttctgccggg ccgacttccc gcatagcggt ggcaagccgt gacagcgcga
       61 ggcccaaagg ccgcagacgg ccgcgaaaaa tgttccggcc gaaattccgc gccacccgct
      121 cgaaatagcg gaattcggcc attggcggca catttggacg cagcgcgcca aggccgctga
      181 tggcgatcag ccggtcagcg ccggcatagg ccacgtttcg ccagcctgtt gcagcgccaa
      241 cgccggttcg gcgggactga gccttaccgc taaggctggc agcggaaagg tcgcgcagtc
      301 ttgcggcgag ttcgggctgg cggcaacggt cgaggatcgt ggcgaaccat gccagccatt
      361 cgccgtcggg gcggctttcc agccgatgga tgctttttcg gtgctttggc agatgatcgg
      421 gttgagcgtc ctgcgacacg ccatcggcca gccagtccag aacatggcgc gcccgcacaa
      481 gttcctcatg cccaaggccc ggatagaaat cccgtatgcg ccgcgcctgg ccaagctcgc
      541 ccgtgcaaat catggaaagc agaaaatagg cccattccac gggatcgggg tcggctgcgc
      601 cataggtcag cgtcgttatt tcgatcagcc tggcaagcga ctcccgggct tggtcggcat
      661 tgccctcatg cagggcgcat aacgccaggc gaaactgcgc ttccggcagg tagctgacat
      721 agtcgaggca ttgcgcgtag cactgccggg cctctgccag ccggccctgt ttcaaaaaga
      781 tgtcgcctcg cgccagcagg gcacggtcgc gtgccgagcc ggaaatatgg atgctgtcgc
      841 gcccggcggc gtgcgtaacc ttttcaaggt cgccaaacgg accggactgg atgattttct
      901 cgccgggctg caagtcccgg ttcagcatat accattggtg gatttgcggc cggtgcttca
      961 gcgtatggcg ggcatggacc agttcatggc cggcttgcgt aatgcgctgc atctcgtcgg
     1021 gatgcgcaaa aagatggtcg atccggtcgg tcacgtcatg atggctggcg aagacacagt
     1081 tttccatgtc caggaatccg gcggcctcca gcgcaggggt acgctcggtc agcaggcaag
     1141 cctttgcgcc aggtatctcg aaatgcttgc gcacgacttc gccgccctgg gttccgcatg
     1201 tcggcacgat aaagctggcg tttagcgctc gcgcataggc ctcgcccgcc agcatcccgg
     1261 cggagcgctt gctggcataa tcgtgttgtg gggaaaccag acaaggatag cgctcgcgga
     1321 taagcgggaa gatttgttgc cgccaggggt aaaggccata gatttggccc gtcagcgtga
     1381 ccggaatggt cttgtgctgg ccatagtcgt ggtagagttc ggggtcgatg aagttcggcc
     1441 agatgaacag gcttgcggca atctcgggcg tataatcggg catcatcgtg ctgatggaaa
     1501 aataggtctc gatgccccac cgctccatgt cggacaggaa gccggcgcgg cggtcgcacc
     1561 atggatcggc attgtgcagg ccaagtttgg gaaccttggc attggcagcc gtattcgtga
     1621 tgatcgtgcg ccgcgaccca tgcgagcgat agccgctttc aaacagcgtc aaatccggct
     1681 gatgccggtc gcatatcgcg gcgtagtcgc agtcctggtt gacgacgacg acctcgaaat
     1741 gcgtcgccag gcatttgacg tgctgctgca tgtgcagcaa cagatacgag gcagcatccg
     1801 cattgggctg gtggtcccac tggaagaaga caagtttcgg cttgcggtgg ccggcatcca
     1861 tgttcatgtc ggcaatcgag ccccttccct tgtgggcaag cccgctgccg ttagcggcac
     1921 gcgcggatga gacatggcct tggcggcttc gaaggggggc gtggcgatgc ctgaaagctt
     1981 tgcctcgaaa taggcgatgg tcttttccag tccctggcgc agcggcacgg ttggctgcca
     2041 gcccagcgcg tgccgcgcgt ggctgatatc cggcttgcgc cgggtcggat catcggccgg
     2101 cagcggcctg aagacgatgg gggaggccga gcctgtcagt tcgatgatca tctcggccag
     2161 ttcgcgcatc tggaattcgc cgggattgcc gagattgacg ggtccggtga tgctgtccgg
     2221 cgcggccatc agccggatga agccgtcgat cagatcctcg acatagcaga aggagcgcgt
     2281 ctgcctgccg tcgccgaaaa gcgtgatcgg ctcgttgcgc agcgcctgca cgatgaagtt
     2341 ggagacgacg cggccgtcat ccgggtgcat gcgcgggcca taggtgttga agatgcgcgc
     2401 gacgcggata ttgacgccgt actggcggtg atagtcgaaa aacagcgttt cggcgcagcg
     2461 cttgccttca tcgtagcagg cgcgcgggcc gatcggattg acgctgccgc gataggcttc
     2521 cgtttgcggg tgcaccgccg ggtcgccata aacctcgctg gtggaggcct ggaaaatctt
     2581 ggccttggtg cgcttggcca ggccaagcag gttgatggcg ccgtgcacgt tggtcttcac
     2641 cgtctgcacc gggtcgaact gatagtgaac cggcgatgca gggcaggcga gattgtagat
     2701 ctcgtcgact tccacataga gcggaaaggt cacgtcatgg cgcaggattt cgaagcgcgg
     2761 gtcgtcgagc agatgcagta ccgtgtcgcg cgagccggta tagtaattgt cgacgcaaag
     2821 gacgtcattg tcctcccgca acagcctttc gcacaggaac gagcccagaa agccggctcc
     2881 gcccgtaacc agaatgcgtt ttcgtccatg catggtgctt ctcctatagg tgccctgcgg
     2941 cgggcagcgg gtcagtacgc cccgttgcgc ctgaagaccg cgggaatggt gctcaagagg
     3001 atgtgccagt cgaaccagag cgagcggttg tcgatgtaga attcgtcgag ctggcgctgt
     3061 agttcgagat cggcgttgct gcgctcggat atttgccaaa gcccggtcaa gccgggcgtt
     3121 accgagcgcc ttttgtggcg gaactcggca ttcatcgcca gaagatggta
//
LOCUS       DASYCS010000352         9238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3287292,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000352 DASYCS010000000
VERSION     DASYCS010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 9238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9238
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3287292"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..865)
                     /locus_tag="VGM46_10980"
     CDS             complement(8..865)
                     /locus_tag="VGM46_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008125359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01459 family HAD-type hydrolase"
                     /protein_id="HEY4193157.1"
                     /translation="MGGPQVLADLHSLAGRYDFFLIDQFGVLHDGTVAYQGAAEALSS
                     LKAMGKKIVLVSNSGKRARQNEDRLRKLGFEGSWDVFLSSGEVAWRKFAGQLGGERLA
                     PGTRCLLLSRGNDRSAIDGLGLDLVTEGAKAEIILLSGSEGDSKPLEFYRALLEPAAR
                     AGVPCICTNPDKIMLTEAGLRFGAGAIADLYEEMGGPVERIGKPFGAIYEAAMEVLGH
                     PAKDRVVGIGDSIEHDIAGANNAGISSVLVRSGILAELGPAELEKAFAQHGATPDFVL
                     PQFIWMAGA"
     gene            complement(1096..1551)
                     /locus_tag="VGM46_10985"
     CDS             complement(1096..1551)
                     /locus_tag="VGM46_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HEY4193158.1"
                     /translation="MLKGIDPLLNADLLHALAAMGHGDDLIVCDSNFPADSIARRTTL
                     GKVLRIDNVTAAQAVAAILSLYPLDTFVDDAASRMEVVGNPAEIPQVQHEVQQVIDRA
                     EGQSRPLQPIERFAFYERAKQAYCIVQTGERRFYGCFALRKGVLPPDAR"
     gene            complement(1581..3008)
                     /locus_tag="VGM46_10990"
     CDS             complement(1581..3008)
                     /locus_tag="VGM46_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006206639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY4193159.1"
                     /translation="MSNHLNFFIDGKWVAPVVPAALDVIDPSTEEAYTSISLGSKADV
                     DKAVAAAKAAFPSFSQTTRTERLKLLKRILEVYNERFEDIAQAVSQEMGAPIAWARDA
                     QAWAGRAHMEATIKALEDYEFSELRGSTMVVKEPIGVAALITPWNWPLNQIVCKVAPA
                     IAAGCTVVLKPSEIAPISGIIFSEVMEAAGTPKGVYNMVNGNGPDVGQIMAGHPDVDM
                     VSFTGSTRAGIIVAKTAADTVKRVAQELGGKSANIILADADFEEAVTKGVEGCFGNSG
                     QSCDAPTRMLVPAARHDEALAIARKAAEAHTVGDPRSEDTRLGPVVSQMQYDKIQRLI
                     EIGIKEGATLVTGGPGRPEHLNRGYYVRPTVFGHVTPGMTIEKEEIFGPVLSVISYED
                     DDDAVKIANDTVYGLAAYVQSKNIDHARAVASRLRAGQINLNYPEWDTFAPFGGYKQS
                     GNGREYADWAIHDFLEVKGIIGYGA"
     gene            3317..4381
                     /gene="fba"
                     /locus_tag="VGM46_10995"
     CDS             3317..4381
                     /gene="fba"
                     /locus_tag="VGM46_10995"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763423.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEY4193160.1"
                     /translation="MARITLRQLLDHAAEHGYGVPAFNINNMEQALAIMEAADASDAP
                     VILQASRGARAYANDIMLKHMMDAVTEIYPHIPVCVHLDHGNEPGTCMTAIQSGFTSV
                     MMDGSLKADGKTPGDWDYNVGVTRTVTTMAHLGGISVEGELGVLGSLETGEGEKEDGH
                     GFEGKLGHDQLLTNPDEAVKFVAETKVDALAIAMGTSHGAYKFTRKPDGKVLAMNVIE
                     EIHRKLPNTHLVMHGSSSVPEELQEIINAYGGKMKPTWGVPVEEIQRGIKNGVRKINI
                     DTDNRMALTGQIRKVLSEDPSEFDPRKYLKPAMAAMTKLCKQRFEEFGTAGHASSIRP
                     IPVAEMARRYKEGKLDPKFG"
     gene            complement(4446..5657)
                     /locus_tag="VGM46_11000"
     CDS             complement(4446..5657)
                     /locus_tag="VGM46_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEY4193161.1"
                     /translation="MNDIRPIRAKSGSNQEGTSAHNRRVMIDALRYNGALSRADLARA
                     TQLTKQAVSNIIDELKRDGFVVALEAVRNGRGQPSTPYRLVPERAFAIGLQIDRHVTR
                     AIIVDLVGNVLVRAQANLPPNDPTQGVSIILELIGSVRSRLAASSPQSETRIVGLGVA
                     MPGPFGLEGFGDSLWDMPAWQKFPLLETLAAGTGLNVGLQNDAAASATAERLVGAAHG
                     VDHAVCLYVGYGIAAGLILNGESYRGGNGNAGEIGKALFSSGGKKQTSLEHRASLASL
                     YKHMGMDAADMHLHERLDELAKAGDASILAWIDDAAPALSWSVHLIETIFDPQTVILS
                     SGAPQSLSNRLFEAMHPLLSSIADRPERPLPRLQPGIADPWAIALGAAAEPISRAFDP
                     RFAAMLKAGSN"
     gene            5802..7037
                     /locus_tag="VGM46_11005"
     CDS             5802..7037
                     /locus_tag="VGM46_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011426606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY4193162.1"
                     /translation="MLKSIGRTLLGATLLGAAFTPHAFAETSLNALFMAQAAYSEADV
                     RAMTDAFTKANPDIKVNLEFVPYEGLHDKTVLAQGSGGYDVVLFDVIWPAEYASNKVL
                     VDVSSRITDDMKKGVLPGAWTTVQNDGKYYGMPWILDTKYLFYNKEILEKAGIKNPPK
                     TWEELSEQAKTIKDKGLLATPIAWSWSQAEAAICDYTTLVSAYGGDFIKDGKPDFQTG
                     GGLEALKYMVDSYKSGVTNPNSKEFLEEDVRKVFENGDAAFALNWTYMYNMANDPKDS
                     KVAGKVGVVPAPGIAGKSEVSAVNGSMGLGVTTASKHPDEAWKFITFMTSQATQNQYA
                     KLSLPIWASSYDDPTVTKGQEELIGAAKLSLAAMYPRPTTPKYQELSTALQQAIQESL
                     LGQSSPEDALKTAAENSGL"
     gene            7110..7994
                     /locus_tag="VGM46_11010"
     CDS             7110..7994
                     /locus_tag="VGM46_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007795756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY4193163.1"
                     /translation="MSGTWMTTRAWLLMLPLLAVMVAVIGWPLVDTVGLSFTDAKLVG
                     TSGHFVGIDNYARMLSSSNFQRTLITTTWFAVVSVTAEMVIGVLTALLLNQQFFGRTA
                     LRALLILPWALPTVVNATLWRLIYNPEYGALNAALSQLGLIDAYRSWLGEPGTALAAL
                     IVADCWKSFPLVALIALAALQAVPRDIIAASLVDGAGPFNRFRFVILPYLAGPLMVAL
                     VLRTIEAFKVFDIIWVMTRGGPANSTRTLSILVYQEAFSFQRAGSGASLALIVTLLVT
                     VLAVAYAALVRKTAGSAG"
     gene            7994..8839
                     /locus_tag="VGM46_11015"
     CDS             7994..8839
                     /locus_tag="VGM46_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018856029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY4193164.1"
                     /translation="MERRSPVFTVFIYACALLLAAIILAPVLWLFIMSISPAADLAAK
                     PLRWWPQHADFSRYAVLLSTAENSAGAAFTSSLRNSIEIAGMATLAALALAVPAGWAV
                     SRTPSVGWSLSMVIATYMLPPVALAVPLYMGLAYLGLLNNVFGLALVYLTILAPFTTW
                     LMKSGFDSIPREIEAAAMIDGAGLFQTWRIITLPLAGPVMATAALFAFLLAWDEFFYA
                     LLFTSDQRAKTLTVAIADLAGGRVSDYGLIATAGVLAALPPVAVGLFMQRALISGLTS
                     GGVKG"
     gene            8836..>9238
                     /locus_tag="VGM46_11020"
     CDS             8836..>9238
                     /locus_tag="VGM46_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HEY4193165.1"
                     /translation="MTMIPTTERPAGLAAIDREMARQNADALASYEQALAIAEKIAAS
                     LKRTGRLLLLGMGGSHAVGRAVEPLYRAHGIDAIALPLSEQLGQPLPLEGRTVLITSQ
                     SGESAEVVRWFSEAGGVLDIFGLTLEENSFLG"
BASE COUNT         1707 a         2978 c         2839 g         1714 t
ORIGIN      
        1 cccgctgtta tgcccctgcc atccagatga attgcggcag cacgaaatcg ggcgtcgcgc
       61 cgtgctgcgc gaacgctttt tccagttcgg cggggccaag ttcggcaagg atgccggaac
      121 gcaccagcac cgacgagatg ccagcgttgt tcgcgcctgc tatgtcgtgc tcgatgctgt
      181 cgccgatgcc gacgaccctg tctttggcgg gatggccgag aacttccatg gccgcctcgt
      241 aaatcgcgcc aaacggcttg ccgatgcgct ccaccggccc gcccatttcc tcatacagat
      301 cggcaatcgc ccccgccccg aagcgcaggc ccgcctcggt cagcatgatc ttgtcagggt
      361 tggtgcagat gcacggcacg ccggcacgcg ccgccggctc cagcaaggca cggtagaact
      421 ccagcggctt gctgtcgccc tcgctgcccg aaagcaggat gatttcggcc ttcgcgccct
      481 ccgtcaccag atcgaggccg aggccgtcga tggctgagcg gtcattgccg cgcgacagca
      541 gcaggcagcg cgtgccgggc gccagccttt cgccgccgag ctggccggcg aacttgcgcc
      601 acgccacttc gccggagctt agaaagacgt cccacgaacc ctcgaagcca agcttgcgca
      661 gccggtcctc gttttgccgg gcgcgcttgc ccgaattgga gacgagcacg attttcttgc
      721 ccatcgcctt caggctggac aatgcttcgg ccgcaccctg ataagcgacc gttccgtcgt
      781 gcagcacgcc gaattgatcg attaagaaaa agtcgtagcg tccagccaga ctgtgaaggt
      841 cagccagaac ctgtggtcca ccgattgcca cgcctaagca ctccatgccg gcacattcga
      901 acgccgaatg ccgcaccggt atcgcgcctt accggatttt ggcgcggcag ccaacgtctt
      961 gagacgaagc ggttcgaagg caggtcgcgc cgctagagcg gttccgattt tgctgtatcg
     1021 atgaacgcgc tctatctctt tgttttggcc gcatttccgg acgcaaaacc gtttcacact
     1081 tttgctggaa atgctttagc gcgcgtccgg cggcaatacg ccttttcgca aggcgaagca
     1141 gccgtagaag cggcgctcgc ccgtctggac gatgcagtag gcctgcttcg cccgctcata
     1201 gaaggcgaag cgctctatcg gctgcaacgg acgggattgc ccttccgcac ggtcgatcac
     1261 ctgctggacc tcatgctgca cctgcgggat ttcggcggga ttgccgacca cttccatgcg
     1321 cgacgcggca tcgtcgacga aggtatcgag cggatagagc gacaggatgg ccgccacggc
     1381 ctgcgccgcc gtgacattgt cgatgcgcag caccttgccc agcgtcgtcc ggcgggcgat
     1441 ggaatcggcc gggaaattgc tgtcgcagac gatgaggtcg tcgccatgcc ccatcgccgc
     1501 cagcgcatgg agcaggtctg cgttaagcag cggatcgatc cccttgagca ctcccggcct
     1561 ccccgatctt gcccgaaggc ttatgcgccg tagccgatga tgcccttgac ctccaggaag
     1621 tcatggatgg cccaatcggc atattcgcgg ccattgcccg attgcttgta gccgccgaac
     1681 ggcgcgaacg tatcccattc cggatagttg aggttgatct gcccggcgcg caagcgggaa
     1741 gcgacggcgc gcgcgtggtc gatgttcttc gactgcacat aggcggcaag gccatagacg
     1801 gtatcgttgg cgatcttcac ggcgtcgtcg tcatcctcat aggaaatgac ggacagaacc
     1861 ggtccgaaga tttcttcctt ttcgatggtc atgccgggcg tgacatggcc gaacaccgtc
     1921 gggcgcacat aatagccgcg gttgagatgt tcgggccggc cggggccgcc cgtcaccagc
     1981 gtcgcgcctt ccttgatgcc gatctcgatc aggcgctgga tcttgtcgta ctgcatctgg
     2041 ctgaccaccg gcccgagcct ggtatcctcc gaacgcggat cgccgaccgt atgcgcctcg
     2101 gcagccttcc tggcgatggc cagcgcctcg tcatggcggg cggcgggcac cagcatgcgg
     2161 gtcggcgcgt cgcaggactg gccgctattg ccgaaacagc cttcgacgcc cttggtgacc
     2221 gcctcctcga aatcggcatc ggcaaggatg atgttggccg acttgccgcc cagttcctgc
     2281 gccacgcgct tgactgtgtc ggccgccgtc ttggcgacga tgatgccggc gcgcgtcgag
     2341 ccggtaaacg aaaccatgtc gacatccgga tggccggcca tgatctggcc gacatcgggg
     2401 ccattgccgt tgaccatgtt gtagacgccc ttcggggtgc cggcggcttc catcacttcg
     2461 gaaaagatga tgccgctgat cggtgcgatc tctgagggct tcagcaccac cgtgcagccg
     2521 gcggcaatcg ccggggccac cttgcagacg atctggttga gcggccagtt ccacggcgta
     2581 atcagcgccg cgacgccgat cggctccttg accaccatgg tcgagccgcg caattcgctg
     2641 aattcatagt cttccagcgc cttgatggtg gcttccatat gcgcgcggcc ggcccaggcc
     2701 tgcgcatcgc gcgcccaggc gatgggcgcg cccatttcct ggctcaccgc ctgcgcgata
     2761 tcctcgaagc gctcgttata gacttcgaga atgcgcttga gcagcttcag gcgctcagtg
     2821 cgcgtcgttt gggaaaagga tgggaacgca gccttcgccg ccgccaccgc cttgtcgaca
     2881 tcggccttcg agccgagcga aatactggta taggcctcct cggtcgaggg gtcgatcacg
     2941 tccagcgcgg caggcaccac cggcgcaacc catttgccgt cgatgaagaa attgagatgg
     3001 ttgctcatgg gttttgctcc gactttcctg gaaggatcga gattggatcg cccaaccttg
     3061 ccgcatctgc cgcttcggct caatggcgca gaattgcgat agggtggacc agtccacgaa
     3121 aaaaccgcgc gatgctgtaa cggctgggcc gtaactgcaa ttctcttgtc gttttcgtcg
     3181 tgcccggcca atgcaacact cgacaaatgc cgggcgtttg gccatgaaca aacccaacgc
     3241 tgacgcggaa acgggcaaat ccgcccaggc cccgcgccgg cgaaacagaa ttgttttcaa
     3301 tcgtcaggga ggacaaatgg cccgcatcac gctcagacaa ctgctcgatc atgccgccga
     3361 acacggctac ggcgtgccgg cgttcaacat caacaatatg gagcaggcgc tggccatcat
     3421 ggaggcggcc gacgcgagcg atgcgccggt catcctacag gcgtcgcgcg gggcgcgcgc
     3481 ctatgccaac gacatcatgc tcaagcacat gatggatgcg gtgaccgaaa tttatccgca
     3541 cattccggtg tgcgtgcatc tggaccacgg caacgaaccc ggcacctgca tgacggccat
     3601 ccagtccggc ttcacctcgg tgatgatgga cggctcgctg aaggccgacg gcaagacgcc
     3661 cggcgactgg gactataatg tcggcgtcac ccgcaccgtc accaccatgg cgcatctcgg
     3721 cggcatctcg gttgagggcg aactcggtgt gctgggcagc cttgagaccg gcgagggcga
     3781 aaaggaagac ggccacggct tcgaaggcaa gctcggccac gaccagttgc tgaccaaccc
     3841 ggacgaggcc gtcaagttcg tcgccgaaac caaagtcgac gcgctggcga tcgccatggg
     3901 cacctcgcac ggcgcctaca aattcacgcg caagcccgac ggcaaggtgc tggccatgaa
     3961 cgtcatcgag gaaatccacc gcaagctgcc gaacacgcat ctggtgatgc acggctcgtc
     4021 ctcggtgccg gaagagttgc aggagatcat caacgcctat ggcggcaaga tgaagcctac
     4081 ctggggtgtg ccggtcgagg aaatccagcg cggcatcaag aacggcgtgc gcaagatcaa
     4141 tatcgacacc gacaaccgca tggcgctgac cggccagatc cgcaaggtgc tgagcgagga
     4201 cccgagcgaa ttcgacccgc gcaaatatct gaagccggcc atggcggcga tgaccaagtt
     4261 gtgcaagcaa cgctttgagg aattcggcac cgccggccat gcctcgtcga tccgccccat
     4321 cccggtggcc gagatggcga ggcgctacaa ggaaggcaag ctcgatccga agttcggctg
     4381 agcgtcctga cccgaaaaag aaagcgccgg ccttcgggcc ggcgtttttg ttcaagccaa
     4441 acgttctagt ttgaaccggc cttgagcatg gcggcaaagc gcgggtcgaa agcacggctt
     4501 atcggctcag ccgctgcgcc caacgcgatg gcccacggat cggcgatgcc aggttgcagg
     4561 cgcggcagcg gccgctccgg acggtcggcg atagaggaca gaagcggatg catcgcctcg
     4621 aacaggcgat tagacaagga ttgcggggcg ccgctggaaa ggatcaccgt ctgcgggtcg
     4681 aagatcgtct cgatgagatg cacgctccag ctcaaggcag gcgccgcgtc gtcgatccag
     4741 gccaggatgc tggcgtctcc tgcctttgcg agttcgtcca gccgttcatg cagatgcata
     4801 tccgccgcgt ccatgcccat gtgcttgtag agcgaggcga gcgaggcacg gtgttcaagc
     4861 gatgtttgct tcttgccgcc agaggaaaac agcgccttgc cgatctcgcc ggcattgccg
     4921 ttgccgccac gataggattc gccgttgagg ataagaccgg ccgcgatgcc gtagccgaca
     4981 tagaggcata ccgcatgatc gacgccgtgc gccgcgccga cgaggcgttc ggcggtggcg
     5041 cttgccgcgg catcgttttg caacccgaca ttcagcccgg tgccggcggc aagcgtttcg
     5101 agcagcggaa atttctgcca ggccggcatg tcccaaaggc tgtcgccaaa accttccagt
     5161 ccgaaaggac cgggcatggc cacgccaagg ccgacgatgc gcgtttcgga ctgtggtgag
     5221 cttgcggcca gcctgcttcg gacgctgccg atcagctcca gaatgatgct gacgccctgc
     5281 gtcggatcgt ttggcggcaa attggcctgc gcccgcacca gcacattgcc gaccagatcg
     5341 acgatgatcg cgcgcgttac atggcggtcg atctgcaagc cgatggcgaa ggcgcgctcg
     5401 ggcacgaggc ggtagggcgt cgagggctgg cccctgccat tgcgcaccgc ttccagcgcg
     5461 accacgaaac cgtcgcgctt caattcatcg atgatgttgg agacagcctg cttggtaagc
     5521 tgggtggcgc gcgcgagatc ggcccgcgac aaggctccgt tataacgcag cgcgtcgatc
     5581 atcacccggc gattatgcgc gctggtgcct tcctgattgc tgccgctctt ggctcggatc
     5641 gggcgaatgt cgttcatcct cgcgtcccct cttgactcga ctaaaagatt gaacaactaa
     5701 taagtcaaga gccttgactt attgcgtatc agcgacggca aagggcgaag ccccttccgg
     5761 cgtcgacaga tcagtaccaa cgtcaaaacg ggaggaagac aatgttgaaa tcgatcggta
     5821 ggacacttct gggcgcaacc cttctggggg ccgctttcac gccgcacgcc tttgccgaaa
     5881 cctcgctcaa cgcgctgttc atggcgcagg ccgcctatag cgaggcggat gtgcgcgcca
     5941 tgacggacgc cttcaccaag gccaatccgg acatcaaggt caacctcgaa ttcgttccat
     6001 acgaagggtt gcatgacaag acggtgctcg cgcaagggtc cggcggctac gacgtggtgc
     6061 tgttcgacgt gatctggccg gctgaatatg ccagcaacaa ggttctggtc gatgtttcct
     6121 cgcgcatcac cgacgacatg aagaagggcg tgctgcccgg cgcgtggacc acggtccaga
     6181 acgacggcaa atattacggc atgccgtgga tcctcgatac gaaatacctg ttctacaaca
     6241 aggaaatcct ggaaaaggcc ggcatcaaga acccgcccaa gacctgggaa gaactgagcg
     6301 aacaggccaa gaccatcaaa gacaagggcc tgctcgccac gccaatcgcc tggagctggt
     6361 cgcaggcgga agccgctatc tgcgactaca ccacgctggt cagcgcctat ggcggcgact
     6421 tcatcaagga cggtaagccg gatttccaga ccggcggcgg gcttgaggcg ctgaaataca
     6481 tggtcgacag ctacaagtcg ggcgtgacca acccgaactc caaggaattc ctcgaagagg
     6541 atgtgcgcaa ggtgttcgag aacggcgacg ccgccttcgc gctcaactgg acctacatgt
     6601 acaacatggc caacgaccct aaggacagca aggtcgcggg caaggtcggc gtcgtgccgg
     6661 cgccgggcat tgccggcaag agcgaggttt cggccgtcaa cggttcgatg ggccttggcg
     6721 tcaccaccgc cagcaagcac ccggacgagg cgtggaagtt catcaccttc atgacctcgc
     6781 aggcgacgca gaaccagtat gccaagctca gcctgccgat ctgggcgtct tcctatgacg
     6841 atccgacggt gaccaaggga caggaagagc tgatcggcgc ggccaagctt agccttgccg
     6901 ccatgtatcc gcgcccgacg acgccgaaat accaggaact gtcgacggct ctacaacagg
     6961 caatccagga atccctgctc ggccagtctt cgcctgaaga cgcgctgaag accgccgccg
     7021 aaaacagcgg cctgtaacac tccgcttgct ccgggcggcg gcaagccgcc cggagcgttc
     7081 catcttttta taaaaggaag ccgcctccca tgtcaggcac ctggatgacc acgcgagcct
     7141 ggctgctgat gctgccgctg ctcgccgtca tggttgccgt catcggctgg ccgcttgtcg
     7201 atacggtcgg tctttccttc accgacgcca agctcgtcgg cacctctggc catttcgtcg
     7261 gcatcgacaa ctacgccagg atgctctcca gctcgaattt ccagcgcacg ctgatcacca
     7321 cgacctggtt cgccgtggtt tcagtcaccg ccgaaatggt catcggcgtg cttaccgcgc
     7381 tgctgctcaa ccagcagttt tttggccgca ccgcgttgcg cgcgctgctg atactgccat
     7441 gggcgctgcc aaccgtcgtc aacgccacgc tgtggcggct gatctacaac cccgaatatg
     7501 gcgcgctgaa cgccgccctt tcgcagctcg gcctgatcga tgcctaccgc tcgtggctgg
     7561 gcgagccggg caccgcgctg gcggcactta tcgttgccga ttgctggaag agctttccac
     7621 tggtggcgct gatcgcgctg gccgccctgc aagcggtgcc gcgcgacatc atcgccgcct
     7681 cgctggtcga tggcgccggg ccgttcaacc gcttccgctt cgtcatcctg ccatacctcg
     7741 ccggaccgct gatggtggcg ctggtgctgc gcaccatcga ggcattcaag gtgttcgaca
     7801 tcatctgggt gatgacgcgc ggcgggccgg ccaactcgac gcggacgctg tcgatcctcg
     7861 tctatcagga agccttttcg ttccagcggg ccggctccgg cgcatcgctg gcgctgatcg
     7921 tcacgctgct cgtcaccgtg ctggcggtcg cctatgctgc attggtgcgc aagacggcgg
     7981 ggagtgccgg ctgatggaac gcagaagccc ggttttcacc gtcttcatct atgcctgcgc
     8041 gctgcttttg gccgccatca tcctcgcgcc tgtgctgtgg ctgttcatca tgagcatttc
     8101 accggcggcc gaccttgccg ccaagccgtt gcgctggtgg ccgcagcatg ccgatttctc
     8161 gcgctacgcc gttcttctat cgacagcgga aaacagcgcc ggcgccgcct tcacctcatc
     8221 gctgcgcaat tccatcgaga tcgccggcat ggcgacgctg gccgccctgg cgcttgccgt
     8281 gcccgccgga tgggcggtgt cgcgcacgcc gtctgtcggc tggtcgctgt cgatggtcat
     8341 cgccacctac atgctgccgc cggtggcgct cgccgtgccg ctctatatgg gcctcgccta
     8401 tctcggcctg ctcaacaatg tgttcgggct ggcgctcgtc tacctcacca tcctcgcccc
     8461 gttcaccacc tggctgatga aatccggttt cgacagcatc ccgcgtgaga tcgaggcagc
     8521 ggcgatgatc gacggcgcgg gccttttcca gacgtggcgc atcatcaccc tgccgttggc
     8581 cggtccggtg atggcgacag cggcactgtt tgccttcctg cttgcctggg acgaattttt
     8641 ctacgcgctt ttgttcacct ccgaccagcg cgccaagacg ctgaccgtcg ccatcgccga
     8701 tctcgccggc ggtcgcgttt ccgattatgg gctgatcgcc accgccgggg ttcttgccgc
     8761 gctgccgccg gtggcggtcg ggcttttcat gcagcgcgcc ctgatttccg gcctgaccag
     8821 cggcggcgta aaaggatgac catgataccg acaacagaac ggccggccgg ccttgccgcc
     8881 atcgaccgtg agatggcgcg ccagaatgcg gacgcgctcg cctcctatga gcaggcattg
     8941 gcgattgccg aaaaaatcgc cgcatcgctg aagcgcacgg gccgcctgct gctgctcggc
     9001 atgggcggtt cgcacgcggt cggccgcgcg gtcgagccgc tttaccgggc gcatggcatc
     9061 gacgccatcg ccctgccttt gtcggaacag cttggccagc cgctgccgct cgaaggccgc
     9121 acggtgctga taacctcgca gtcgggcgaa agcgccgagg tggtgcgctg gttctccgaa
     9181 gcgggcggcg tgctcgacat tttcgggctg acactggaag aaaattcgtt tctcgggc
//
LOCUS       DASYCS010000353         5094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1696299,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000353 DASYCS010000000
VERSION     DASYCS010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 5094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5094
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1696299"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /gene="phnK"
                     /locus_tag="VGM46_11025"
     CDS             <1..736
                     /gene="phnK"
                     /locus_tag="VGM46_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008123404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HEY4193166.1"
                     /translation="NYGARAGCTDVSLDIWPGEVLAVVGESGSGKTTLLNCVSTRLEP
                     TAGSIRYRMRDGELRDLTRMGEAERRHLLRTDWGFVHQNPADGLRMTVSAGANVGERL
                     MAIGARHYGNIRATATDWLGRVEIDAGRIDDQPRAFSGGMRQRLQIARNLVTAPRLVF
                     MDEPTGGLDVSVQARLLDLLRGLVSDLNLAVVIVTHDLAVARLLSHRMMVMKDGRVIE
                     HGLTDRVLDDPQAPYTQLLVSSILQV"
     gene            744..1451
                     /gene="phnL"
                     /locus_tag="VGM46_11030"
     CDS             744..1451
                     /gene="phnL"
                     /locus_tag="VGM46_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HEY4193167.1"
                     /translation="MSPMLTVSGLTKTFTMHLRGGIELPVIEGAAFALHSGTCAVLGG
                     PSGAGKSSILKILYGNYGASAGKILVRHGDREIDLVAAGARMVLELRRDTIGYVSQFL
                     RVVPRVSALDIVAEPLVERGGDRQKAALRAGELLSRLNLPERLWGLPPATFSGGEQQR
                     VNIARGFITGHPILLLDEPTASLDAANRAVVVEMIAEKKKAGAALLGIFHDAEVRAAV
                     ADVVIDVTGFSTSRKAA"
     gene            1454..2149
                     /locus_tag="VGM46_11035"
     CDS             1454..2149
                     /locus_tag="VGM46_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019996618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HEY4193168.1"
                     /translation="MTRLSETPLVHPTARVENCQLGRYTEVAEGGRLTETELGDYSYV
                     MEDCRSWCVTIGKFANIAASVRINATHHPMSRATLHHITYRASDYFEDAEHEADFFAA
                     RRAKRVFIGHDTWIGHGVTILPGVRIGNGAVVGAGAVVTKDVAPYTVVGGVPARLIRQ
                     RFAPAIGERMDALAWWHWPHERLRAALDDFRDLDAEAFLARHEKARRASAVDRHETDM
                     QPTPALHAARLGL"
     gene            2298..3137
                     /gene="phnC"
                     /locus_tag="VGM46_11040"
     CDS             2298..3137
                     /gene="phnC"
                     /locus_tag="VGM46_11040"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HEY4193169.1"
                     /translation="MTAISIRSLSKNFGKKRALDNVSLEIQRGEMVALIGASGSGKST
                     LLRHICGLEIGQAGQSTVEVMGSPIQNNGRLSGRARQMRRNIGVVFQQFNLVNRLSVL
                     SNVLLGNLGHIPAWRGTLGLFSRQEKHAAREALARVGIPEVAWQRASTLSGGQQQRAA
                     IARTLIQRSNILLADEPIASLDPSSARRVMEILAAVNRDEQITCVVSLHQVEYARRYC
                     PRTVALRDGRIVFDGPSTDLTNQMLVELYGDNSEELILPDAPPQPSKPKRASEPDRVA
                     AFA"
     gene            3169..4158
                     /gene="phnD"
                     /locus_tag="VGM46_11045"
     CDS             3169..4158
                     /gene="phnD"
                     /locus_tag="VGM46_11045"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY4193170.1"
                     /translation="MNAFLKGVAAAAFAMTLSGTALAEEINFGIISTESQQALKPKWE
                     PLLAELSKQTGLDIKPFFASDYAGVIEGMRFGKVQLAWYGNKAAMEAVDRADGEIFVQ
                     SVSSTGDPGYWSLILVPKDSKLTSLDDLLTCDKTLNFGLGDPNSTSGYLVPMTFIFGA
                     RHIDPKNCFKNLTNANHETNAMAVANGQVDAAANNTENLALIEKNQPEAFKNIKVLWK
                     SPLIPSDPLVWRKDLPDADKAKIKEFFLTYGTDKSKGDAAKEKAVLAGLAWAPFVAST
                     NDQLLPVRVMELSKSISLLESDASLSDADKKAQIEKLEAEKAGYEQKLKANQS"
     gene            4248..5066
                     /gene="phnE"
                     /locus_tag="VGM46_11050"
     CDS             4248..5066
                     /gene="phnE"
                     /locus_tag="VGM46_11050"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HEY4193171.1"
                     /translation="MSQTTEISRVAPVRVDVERDWARSAWTTATWVILLVLLVWSWAP
                     AEMSRAVYLYTDGGNMAKYLKGFAHPDFSDWSYYVGEMVVTLQIALWGTFLAAVFAVP
                     FSILSARNIAPWYIVQPVRRVMDACRSIHELVFAVLFVVAVGLGPFAGVMALFVHTTG
                     ILAKLFSEAVEAIDARPVEAIRATGASRLQEVVFGVVPQVMPLWLSFVLYRFESNVRS
                     ATVLGVIGAGGIGQVLFESVRGFYYPQASAMLIIMLVTVSLIDVLSQQMRKLFL"
BASE COUNT          879 a         1756 c         1563 g          896 t
ORIGIN      
        1 gaactacggc gcgcgcgccg gctgcaccga tgtcagcctc gacatctggc cgggcgaggt
       61 gctggcggtg gtgggcgaat ccggttccgg caagacgacc ttgctcaact gcgtctccac
      121 ccggctggag cctaccgccg gctccatccg ctaccgaatg cgcgacggcg aactgcgcga
      181 tcttacccgc atgggcgagg ccgagcgccg ccatctcctg cgcaccgact ggggcttcgt
      241 acaccagaac ccggcggatg gcctgcgcat gacggtatcg gcgggcgcca atgtcggcga
      301 gcggctgatg gccatcggcg cgcgtcacta cggcaatatc cgcgccacgg ccaccgactg
      361 gctcggccgc gtcgagatcg acgccgggcg catcgacgac cagccgcgcg ccttctccgg
      421 cgggatgcgc cagcgtttgc agatcgcgcg caatctcgtc accgcgccgc gcctcgtctt
      481 catggacgag ccgaccggcg ggctggacgt ttcggtgcag gcgcggctgc tcgacctgct
      541 gcgcggcctg gtcagcgacc tcaacctcgc cgtggtcatc gtcacccacg atctcgccgt
      601 ggcgcggctt ttgtcgcacc gcatgatggt gatgaaggac ggccgcgtca tcgagcatgg
      661 gctgaccgac cgcgtgctgg acgatccgca ggcgccctac acccagcttc ttgtttcctc
      721 gatcctgcaa gtctgaggcc gccatgtcgc ccatgctgac cgtttccgga ctgaccaaga
      781 cattcaccat gcacctgcgc ggcggcatcg agctgccggt catcgaaggc gcggccttcg
      841 ctctccattc cggcacctgc gcggtgctgg gcggcccttc gggcgccggc aagagttcga
      901 tcctgaagat actctacggc aattacggcg ccagcgccgg caagatcctg gtgcgccacg
      961 gagaccgcga gatcgacctg gtcgccgccg gcgcgcgcat ggtgctggaa ctgcgccgcg
     1021 acaccatcgg ctatgtcagc cagttcctgc gcgtggtgcc gcgcgtcagc gcgctggaca
     1081 tcgtcgccga gccgctggtc gagcgcggcg gcgacaggca aaaggctgcg ctgcgcgcgg
     1141 gcgaactcct gtcgcggctc aatttgccgg agcggctgtg gggcctgccg ccggcgacgt
     1201 tttcgggcgg cgaacagcag cgcgtcaaca tcgcgcgcgg cttcatcacc ggccacccca
     1261 ttcttctgct ggacgagccg acggcctcgc tcgacgccgc caaccgggcg gtggtggtgg
     1321 agatgatcgc cgaaaagaag aaagcgggcg ccgccctgct cggcatcttc cacgacgccg
     1381 aagtgcgcgc cgccgttgcc gatgtcgtca tcgacgtcac cggtttttcc acatccagaa
     1441 aggcggcgtg accatgaccc ggctgtccga gacccccctt gtccacccga ccgcacgggt
     1501 cgagaactgc cagctcggcc gctatacgga agtggccgag ggcggccgcc tgacggagac
     1561 ggaactgggc gactattcct atgtgatgga agattgccgc agctggtgcg tcaccatcgg
     1621 caagttcgcc aacatcgccg cgtcggtgcg catcaacgcc acccatcatc cgatgtcgcg
     1681 cgcgacgctg caccacatca cctaccgcgc ctccgattat ttcgaagatg ccgagcatga
     1741 ggccgacttc ttcgccgcgc gacgcgcaaa gcgcgtcttc atcggccacg acacctggat
     1801 cggccatggc gtcaccatcc tgcccggcgt gcggatcggc aatggcgccg tggtcggcgc
     1861 gggcgcggtg gtgaccaagg acgtcgcgcc ctacacggtg gtcggcggcg tgccggcgcg
     1921 cctcatccgc cagcgtttcg cgcccgccat cggcgagcgc atggatgcgc tggcctggtg
     1981 gcactggccg catgagcgcc tgcgcgccgc gctggacgat ttccgcgatc tcgacgccga
     2041 agccttcctg gcgcgccatg aaaaggcccg tcgcgcttcg gctgtggatc gtcacgaaac
     2101 agacatgcaa ccgacacctg cgcttcacgc ggcaaggcta ggcctatgaa cgcaccacgc
     2161 aggatcgcga tgccgcccgc cccagccgcg aaagcagcaa cggcggccgc tgcctgccgc
     2221 ccggccggtt tctcgaacgc atcgctaatc tctgaggatg cacggccatt ttctaaccgc
     2281 atggaaggag cgacgccatg accgccatct ccattcgcag cctgtcgaag aacttcggca
     2341 agaaacgcgc cctcgacaat gtcagcctcg aaatccagcg cggcgaaatg gtcgcgctga
     2401 tcggtgcttc cggttccggc aagagcacct tgctgcgcca catatgcggc ctcgaaatcg
     2461 gccaggcggg ccagagcacc gtcgaggtga tgggcagccc gatccagaac aatggccgcc
     2521 tttcggggcg cgcccgtcaa atgcgccgca atatcggcgt cgtcttccag cagttcaacc
     2581 tggttaaccg gctgtcggtg ctgtccaacg tgctgctcgg caatcttggc cacataccgg
     2641 cctggcgcgg cacgcttggc ctgttcagca ggcaggaaaa gcacgcggcg cgtgaagcgc
     2701 tcgcccgcgt cggcattccc gaagtcgcct ggcagcgggc ctcgacgctc tccggcggcc
     2761 agcagcagcg cgccgccatc gcccgcacgc tgatccagcg ttccaacata ttgctggccg
     2821 acgagccgat cgcctcgctg gacccctcct ctgcccggcg cgtgatggag attctcgccg
     2881 ccgtcaaccg cgacgagcag atcacctgcg tggtgtcgct gcaccaggtc gaatatgcgc
     2941 gccgctattg cccgcgcacg gtggctcttc gcgatggccg catcgtcttc gacgggccgt
     3001 ccaccgacct taccaaccag atgctcgtcg agctttacgg cgacaattcc gaagaactga
     3061 ttttgccgga tgcacccccg cagccgtcga aaccgaagcg cgcctccgaa ccggaccgcg
     3121 ttgcggcctt cgcctaacca accaaaccaa tcaacaggga agcttgttat gaacgcattt
     3181 ctgaaaggcg tggcagccgc cgcctttgcc atgacgctgt ctggcacggc gcttgccgaa
     3241 gagatcaatt tcggcatcat ctccaccgaa tcccagcagg cgctgaagcc caaatgggag
     3301 ccgctgctgg cggaactttc caagcagacc ggcctcgaca tcaagccgtt cttcgcttcc
     3361 gactatgccg gcgtcatcga aggcatgcgc ttcggcaaag tgcagcttgc ctggtacggc
     3421 aacaaggccg ccatggaagc cgtcgaccgc gccgacggcg aaatcttcgt ccagagcgtc
     3481 tcgtccaccg gcgatcccgg ctattggtcg ctgatcctcg tgcccaagga cagcaagctg
     3541 acctcgctcg acgacctgct gacatgcgac aagacgctga atttcggcct cggcgatccg
     3601 aactcgacct cgggctatct cgttccgatg accttcatct tcggcgcgcg ccacatcgat
     3661 ccgaagaact gcttcaagaa cctcaccaac gccaaccatg agacgaacgc catggcggtc
     3721 gccaacggcc aggtcgatgc ggccgccaac aacaccgaaa acctggcgct gatcgagaag
     3781 aaccagccgg aagccttcaa gaacatcaag gttctgtgga agtcgccgct gatcccgtcc
     3841 gatccgctgg tctggcgcaa ggacctgccc gatgcggaca aggccaagat caaggaattc
     3901 ttcctgacct acggcaccga caagtcgaag ggtgacgccg ccaaggaaaa ggccgtgctg
     3961 gccggtctcg cctgggctcc gttcgtggct tccaccaacg atcagctgct gccggtccgc
     4021 gtcatggaat tgagcaagtc gatctcgctg cttgaatccg acgcctcgct ctccgacgcc
     4081 gacaagaagg cgcagatcga gaaactggaa gccgaaaagg ccggctacga gcagaagctc
     4141 aaggccaacc agagctgatc cagccggccc ggccgtttcc ctatccatac actccctttg
     4201 ccggccgcct cgaccaggcg gccggttcca ccagccgaga tgcaacgatg agccagacga
     4261 ccgaaataag ccgcgtcgcg cctgtccgcg tcgatgtcga gcgtgactgg gcaagaagcg
     4321 cgtggaccac cgcgacctgg gttattctgc tggtgcttct ggtgtggagc tgggcgccgg
     4381 cggaaatgtc gcgcgcggtc tatctctaca ccgacggcgg caatatggcg aagtatctca
     4441 aggggttcgc ccacccggac ttctccgact ggtcctatta tgtcggggaa atggtcgtca
     4501 ccttgcagat cgcgctgtgg ggcactttcc tggcggcggt attcgcggtt ccgttttcca
     4561 tcctgtcggc gcgcaacatc gcgccctggt acatcgtcca gccggttcgc cgcgtcatgg
     4621 acgcctgccg ctccatccat gaactggtct ttgcggtgct gttcgtcgtc gcggtcgggc
     4681 ttggcccgtt cgccggcgtc atggcgctgt tcgtccacac caccggcatt ctggccaagc
     4741 tgttttccga ggcggtcgag gccatcgatg cccgccccgt cgaggcgatc cgcgccaccg
     4801 gcgcttccag gcttcaggaa gtggtcttcg gcgtcgtgcc gcaggtcatg ccgctatggc
     4861 tgtcctttgt gctttaccgg ttcgaatcca acgtgcgcag cgccaccgtg cttggcgtca
     4921 tcggggccgg cggcatcggc caggtgctgt ttgaatccgt ccgcggcttc tactatccgc
     4981 aagcctcggc catgctcatc atcatgctgg tgacggtttc gctgatcgac gtcctgtcgc
     5041 agcagatgcg caagctgttc ctgtgatgcg ctacgcggtc tatttcacgc cggc
//
LOCUS       DASYCS010000354         3108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1111975,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000354 DASYCS010000000
VERSION     DASYCS010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1111975"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VGM46_11055"
     CDS             complement(<1..204)
                     /locus_tag="VGM46_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY4193172.1"
                     /translation="MRNHIRWHIAVFLAPAVLVYTAIMILPLAGTLQFSLFRNVDQSQ
                     VFVGFDNFRTLFGDPRWSAGFWNA"
     gene            complement(207..848)
                     /locus_tag="VGM46_11060"
     CDS             complement(207..848)
                     /locus_tag="VGM46_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193173.1"
                     /translation="MTPQELAKKHPVLYHITSAGAAKHILARGLLTTSDILDRWEVEA
                     GYRLELTRCRRKTDVTLHHPDNGVIVVSNNEPLSELKLQSILDDGLTSGDWLEMLNSR
                     VFFFVDQKQLSRLANSRSNSSLSKDVLVIDTKRLADAYFEKIEISPINSGNTNYDAVR
                     RGRSTFTKLRGTSFELWRYSRGNKSADKISEVCIPSSILDVTDFVVEIREAKA"
     gene            complement(904..2163)
                     /locus_tag="VGM46_11065"
     CDS             complement(904..2163)
                     /locus_tag="VGM46_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012758334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY4193174.1"
                     /translation="MQRKLLTALLLGSSILGSSAAAFAEDVTLTIESWRNDDTQIWQT
                     QIIPAFEKANPGIKVVFAAAAPTEYNAALNAKLAAGSAGDLITCRPFDASLDLYNKGY
                     LSAVDDVDGMKNFSDVAKAAWQTDDGKATFCVPMASVIHGFIYNKDAFDKLGITPPKT
                     NDEFYADLQKIKDDGTFIPLAMGTKDQWEAATMAYQNIGPSYWHGEEGRKALIGGKQK
                     LTDADWVAPFTELAKWGPFMGDGFQAQAYPDSQNLFTLGRAAIYPAGSWEISVFNTQA
                     QFKMGAFPPPLPKEGDKCYLSDQPDHAIGVNAKSAHPEEARKFLSWVASKDFAEIYGN
                     ALPGFFPLTSETVELKDPLAKEFLSWRQSCEPTMRSTYQILSRGTPNLENETWVASAN
                     VINGTETPEAAAKKLQDGLDSWYKPAK"
     gene            complement(2220..>3108)
                     /locus_tag="VGM46_11070"
     CDS             complement(2220..>3108)
                     /locus_tag="VGM46_11070"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEY4193175.1"
                     /translation="EALHHEAKGLDVQAPDAILASLVNAQVEAACAVRNAIPAIAAAA
                     EIVAERLKRGGKLAYAAAGSSGLMALADALELPGTFGISRDRIAILIAGGDEAFRTLA
                     GGPEDDTQEATKAVADAALGPGDCLIAVSASGTTPYAVRALEEAKRRGAATIAIANNR
                     GTSLLNLADAAILLETPPEVIAGSTRMGAGTAQKIALNMLSTLAAIHLGHVHDGYMVN
                     LYADNIKLRDRAARIVSAISGRDRAEAAQLLDRSGGVVKVAILLAAGADSTEAAQEIL
                     EGTGQRLRPALSVIEGSRR"
BASE COUNT          557 a          927 c          964 g          660 t
ORIGIN      
        1 cgcgttccag aagccggcag accagcgcgg gtcgccaaac agcgtgcgga aattgtcgaa
       61 gccgacgaat acctgcgact ggtcgacatt gcgaaacagc gagaattgca gcgtgccggc
      121 cagcggcagg atcatgatcg ccgtatagac caggaccgcc ggcgccagga acacggcgat
      181 atgccagcgg atatggtttc gcatcctcaa gcctttgctt cacggatttc gacgacaaaa
      241 tctgtgacgt cgagaatcga actgggtata cacacttcac taattttatc ggccgattta
      301 ttgccacggg aatagcgcca caactcgaag ctcgtaccac gcagcttggt aaaggtactt
      361 cgccccctcc taactgcatc atagttcgta ttgccggaat ttatcgggct gatttcaatc
      421 ttttcgaagt aggcatcggc aagccgtttg gtgtcgatca ctagaacatc cttagacagg
      481 ctgctgttcg agcgggaatt cgccaatcgg ctcagctgct tttggtccac aaagaagaaa
      541 accctactat taagcatttc aagccaatcg cctgacgtca gcccatcgtc gaggatcgac
      601 tgtaatttca attcgctgag gggctcattg ttactcacta caatcacgcc attatctggg
      661 tgatgcaggg tgacatcagt cttgcggcga catctggtaa gttcaaggcg ataaccagct
      721 tcaacttccc agcgatccag aatatcgctg gttgtgagca gccccctggc aagaatgtgc
      781 tttgccgcac cggcggaggt gatgtggtat aaaaccgggt gctttttggc caattcttga
      841 ggtgtcatcc attaacgccg cgcgagaggt gccctgccgg ttttgggcag ggcaactcat
      901 ttcctacttg gccggcttgt accagctgtc caggccgtct tgcagcttct tggcggcggc
      961 ttccggggtt tcggtgccgt tgatcacatt ggccgaggca acccaggtct cgttttcgag
     1021 gttaggcgtg ccgcgcgaca ggatctggta ggtcgagcgc atggttggct cgcagctctg
     1081 gcgccagctc aggaattcct tcgccagcgg gtccttcaac tcgaccgtct cgctggtgag
     1141 cgggaagaag ccaggcagcg cgttgccgta gatttcggcg aagtccttcg aagccaccca
     1201 cgacaggaac ttcctggctt cttcgggatg cgccgacttg gcgttgacgc cgatggcgtg
     1261 gtcaggctgg tcggaaaggt agcacttgtc gccttccttg ggcagcggcg gcgggaaagc
     1321 gcccatcttg aactgcgcct gcgtgttgaa cacggagatt tcccatgagc cggcggggta
     1381 gatcgcggcg cggccgagcg tgaacaggtt ctggctgtcg ggataggcct gcgcctggaa
     1441 gccgtcgccc atgaacggac cccacttggc cagttccgtg aagggcgcga cccaatcggc
     1501 gtcggtcagc ttctgcttgc cgccgatcag cgccttgcgg ccttcctcgc catgccagta
     1561 gctggggccg atgttctggt aagccatggt ggcggcttcc cactggtcct tggtgcccat
     1621 ggcgagcgga atgaacgtgc cgtcgtcctt gatcttctga aggtcggcgt agaactcgtc
     1681 attcgtcttc ggcggcgtga tgccgagctt gtcgaaggcg tccttgttgt agatgaagcc
     1741 atggatgacg gatgccatcg gcacgcagaa cgtcgccttg ccgtcgtcgg tctgccacgc
     1801 cgccttggcg acgtcggaga agttcttcat gccgtcaaca tcgtcgaccg cgctgagata
     1861 gcccttgttg tagaggtcga gcgaagcgtc gaaggggcgg caggtgatca ggtcaccggc
     1921 ggagcctgcg gcgagcttgg cgttgagcgc cgcgttatat tcggtcggcg cggctgcggc
     1981 gaacaccacc ttgatgccgg gattggcctt ctcgaaggcc gggatgatct gcgtctgcca
     2041 gatctgcgta tcgtcgttgc gccagctttc aatggtcagc gtcacgtctt cggcgaaagc
     2101 cgcggcgctg gaacccagaa tgctggaccc cagcagaagc gccgtcaata atttgcgttg
     2161 catgttcatt ctccctgttg acctttatca ggttcggttt tgatgagaac ggcggttttc
     2221 taccgcctgc tcccctcgat gacggaaagg gccggccgca gcctttggcc ggtcccctcc
     2281 agtatttctt gcgcggcctc ggtactgtcg gccccggcgg caagcaggat cgccaccttc
     2341 accacgccgc cgcttcggtc cagcaactgc gccgcctcgg cccggtcgcg cccgcttatg
     2401 gcggaaacga tacgcgccgc gcggtcgcgc agcttgatgt tatcggcata gagattgacc
     2461 atatagccgt catggacatg gccaagatgg atggccgcca gcgtcgacag catgttgagg
     2521 gcgatcttct gcgccgtgcc ggcgcccatg cgcgtcgagc cggcgatcac ttccggaggc
     2581 gtctcaagca ggatggcggc gtcggcgagg ttcagcagcg acgtgccgcg attattggcg
     2641 atggcgatgg tggcggcacc tcggcgcttg gcttcttcca gcgcccgcac cgcgtagggc
     2701 gtcgtcccgc tggccgaaac ggcaatcagg cagtcgccgg ggccaagcgc ggcatccgcg
     2761 acggccttcg tggcctcttg cgtatcgtct tccggtccgc cggccaatgt cctgaaggcc
     2821 tcgtcgccgc cggcgatcaa aatggcgatg cggtctcgcg aaatgccgaa agtgcccggt
     2881 aattccagcg catcggccag cgccatcagg cctgaactgc cagccgccgc ataggcaagc
     2941 ttgccgccgc gcttcagcct ttcggccacg atctcggcgg cggcggcgat ggctggaatg
     3001 gcgttgcgca cggcgcaagc cgcttctacc tgcgcgttga ccagggaagc aaggatagcg
     3061 tccggagcct ggacgtccag gcccttcgcc tcatggtgca gcgcttcg
//
LOCUS       DASYCS010000355         3591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4879892,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000355 DASYCS010000000
VERSION     DASYCS010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4879892"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="VGM46_11075"
     CDS             <1..443
                     /locus_tag="VGM46_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HEY4193176.1"
                     /translation="DALLDERKRLERELNEARKKLALGGGSSTDSVGESETVAGVGFM
                     GKAVSGISPKDLKPLADAGKQALGSGVVVFVGAGEDNKASVVVAVTDDLVGRFSAVEL
                     VRVASAALGGQGGGGRPDMAQAGGPDASKAGEAIAAVKAALQAA"
     gene            complement(581..1792)
                     /locus_tag="VGM46_11080"
     CDS             complement(581..1792)
                     /locus_tag="VGM46_11080"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017964507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEY4193177.1"
                     /translation="MAKIKVANPVVELDGDEMTRIIWQFIKDKLIHPYLDINLDYYDL
                     GVEHRDATNDQVTIDAAHAIQKHGVGVKCATITPDEQRVEEFKLKKMWKSPNGTIRNI
                     LGGTIFREPIIMKNVPRLVPGWTKPIIVGRHAFGDQYKATDFRYPGKGKLSIKFVGED
                     GKVIEHDVYDAPGAGVAMAMYNLDDSIREFARASLNYGLLRGYPVYLSTKNTILKAYD
                     GRFKDLFQEVFEQEFADAFKEKKITYEHRLIDDMVASSLKWSGGYVWACKNYDGDVQS
                     DTVAQGFGSLGLMTSVLMTPDGKTVEAEAAHGTVTRHYRQHQKGQETSTNSIASIFAW
                     TRGLAHRAKLDDNAELARFSATLEKVCIQTVESGFMTKDLSLLIGPDQPWLSTAGFLD
                     KIDENLQKAMA"
     gene            2017..2892
                     /locus_tag="VGM46_11085"
     CDS             2017..2892
                     /locus_tag="VGM46_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEY4193178.1"
                     /translation="MSDTKIKPSPNRIGPAIILVEPQLGENIGMVARAMANFGLAELR
                     LVNPRDGWPSEKARAAASNADHVIDATVVFDDLASAVADLNFVFATTARQRDGFKEVR
                     GPVEAGRSLRARGLSGQRTGILFGRERFGLYNEEVALADEIVTFPVDPGFASLNIAQA
                     VLLMSYEWMKSGLEQETATNFSAPVMKPSSKEQLHGLFAHLEAALDARGYFRPLPKKP
                     KMVDNLRSVLTRPGFAEAELKVLRGVVASLDYFSPKEPRGAGYPKRKEEVDRRMRKGG
                     GGKAASPAGEDADND"
     gene            2885..>3591
                     /gene="murI"
                     /locus_tag="VGM46_11090"
     CDS             2885..>3591
                     /gene="murI"
                     /locus_tag="VGM46_11090"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEY4193179.1"
                     /translation="MTDRPILLFDSGIGGLTVLREARVLMPERRFVYVADDAAFPYGD
                     WEEDALRQRIVKLVGELIARHDPELFVIPCNTASTLVLSDVREAYPDTPFVGTVPAIK
                     PAAERTRSGLVSVLATPGTVKRQYTRDLITQWAAKCHVRLVGSQCLARLAENYVREGF
                     VDEEAVRREIEPCFIEQDGRRTDIVVLACTHYPFLVNRMRKTAPWPVDWLDPAEAIAR
                     RALSLLGGRGSGDGVHEG"
BASE COUNT          639 a         1071 c         1180 g          701 t
ORIGIN      
        1 tcgacgcatt gcttgacgag cgcaagaggc ttgagcggga actgaatgag gctcgcaaga
       61 agctggcgct cggcggcggt tcgtccaccg acagcgtcgg cgaaagcgaa accgtcgctg
      121 gcgtgggctt catgggcaag gccgtcagcg gcatttcgcc gaaagatctg aagccgctgg
      181 ccgatgctgg caaacaggcg ctgggttccg gcgtcgtcgt atttgtcggc gccggcgagg
      241 acaacaaggc aagcgtcgtc gtcgcggtca ccgacgatct ggtcgggcgc ttcagcgcgg
      301 tcgaactggt gcgggtcgca tccgcggcgc tcggcggtca gggcggcggc ggccgtcccg
      361 acatggcgca ggccggcggg ccggacgcct cgaaagccgg cgaggcgatc gccgcggtca
      421 aggcagccct tcaggccgcc tgaaccgaag ctttggagca tttccagcag tttttcgccc
      481 ggaaatgcag ccaaaacaaa gagatagagc ggttttgtcg attggataaa accagaaccg
      541 ctttagaaat aaacaaaagc cggcgtaacg gcgaggacgc ttatgccatc gccttctgca
      601 aattctcgtc gatcttgtcg aggaagccgg cggtggaaag ccacggttgg tcggggccga
      661 tcagcaacga caaatccttg gtcatgaagc cgctttcgac cgtctggatg cagacctttt
      721 ccagcgtggc ggagaagcgc gccagctctg cgttgtcatc cagcttggcg cgatgcgcca
      781 ggcctcgcgt ccaggcgaag atcgaggcga tggagttggt ggaggtctcc tggcccttct
      841 gatgctggcg gtagtggcgg gtgacggtgc cgtgcgcggc ctccgcttcc accgtcttgc
      901 cgtccggcgt catcagcacc gaggtcatca ggccgagcga gccgaagccc tgcgcaaccg
      961 tgtcggactg cacgtcgccg tcgtaattct tgcaggccca gacatagccg cccgaccatt
     1021 tcaggctgga agccaccatg tcgtcgatca ggcgatgttc gtaggtgatc ttcttttcct
     1081 tgaacgcatc ggcgaattcc tgctcgaaca cttcctggaa caggtccttg aagcggccgt
     1141 cataggcttt caggatggtg ttcttggtcg aaagatagac cgggtaaccg cgcagcaggc
     1201 cataattcag cgaggcgcgg gcgaattcgc ggatcgaatc gtcaaggttg tacatggcca
     1261 tggcgacgcc ggcgccgggc gcatcgtaaa cgtcatgttc gatgaccttg ccgtcctcgc
     1321 cgacgaactt gatcgaaagc ttgcccttgc ccggatagcg gaagtcggtg gccttgtact
     1381 ggtcgccgaa ggcgtggcgg ccgacgatga tcggcttcgt ccagcccggc accaggcgtg
     1441 gcacgttctt catgatgatc ggctcgcgga agatggtgcc gccgagaatg ttgcggatgg
     1501 tgccgttggg cgacttccac atcttcttga gcttgaattc ctcgacacgc tgctcgtcgg
     1561 gggtgatggt ggcgcatttc acgccgacgc cgtgcttctg gatggcgtgg gcggcgtcga
     1621 tggtcacctg atcgttggtg gcgtcgcggt gctcgacgcc gagatcgtaa taatcgaggt
     1681 tgatgtcgag ataggggtgg ataagcttgt ccttgatgaa ctgccagatg atgcgggtca
     1741 tctcgtcgcc gtcgagttcg acgaccgggt tcgccacctt tatcttcgcc atggaaaaat
     1801 tgcctcgctg ctaaggatcg aacggcctgc cagcatggct tcggccggga tgcggcccct
     1861 ataccaaagc ccttcgggct gcgcaaacgt tgacggcggt ttggccggcg cgatgaaagc
     1921 aaatgaacat cggcatccgg ctttgcgaat gcttcatttt ccgggctgaa tatgtcacga
     1981 cgccgaaatg ccacaaaaac tgtttcattg agcatcatgt ctgataccaa gataaagcca
     2041 tcgcccaacc gtattggccc ggcgatcatc cttgtcgaac cgcagcttgg cgagaatatc
     2101 ggcatggtcg cccgcgccat ggccaatttc ggcctggccg aacttcgcct cgtcaacccg
     2161 cgcgacggct ggcccagcga aaaggcgcgc gctgccgcca gcaatgccga tcatgtgatc
     2221 gacgcgacgg tggttttcga cgacctcgcc tcggcggttg ccgatctcaa tttcgttttc
     2281 gccaccacgg cgcgccagcg cgacggcttc aaggaagtgc gcggtccggt cgaggcgggc
     2341 aggtcgctgc gggcgcgtgg cctgtccggg caacgcaccg gcatcctgtt cgggcgcgag
     2401 cggttcgggc tttataacga ggaagtggcg cttgcagacg agatcgtcac ctttccggtc
     2461 gatccgggct ttgcctcgct caacatcgcg caggcggtgc tgttgatgtc ttatgaatgg
     2521 atgaaatccg ggctggagca ggaaacggcg acgaatttct ccgctcccgt catgaagccg
     2581 tccagcaagg agcaattgca cggcctgttc gcccaccttg aagcggcgct cgatgcgcgc
     2641 ggctatttca ggccgctgcc caagaagccc aagatggtgg acaatctgcg ctcggtgctg
     2701 acgcgtccgg gctttgccga agccgaactc aaggtgttgc gcggcgtggt cgcctcgctc
     2761 gactattttt cgcccaagga accacgcggc gccggctatc ccaaacgtaa ggaagaggtc
     2821 gaccggcgca tgcgcaaggg cggcggcggc aaggcggcgt cgccggctgg cgaggacgcc
     2881 gacaatgact gaccggccca tcctgctgtt tgattccggc attggcggcc tgacggtgct
     2941 gcgcgaggcg cgcgtgctga tgccggagcg gcgcttcgtc tatgtcgccg atgacgccgc
     3001 ctttccctat ggcgactggg aggaggatgc gctgcgccag cgcatcgtca agctggttgg
     3061 cgaactgatc gccagacacg atcccgagct tttcgtcatt ccctgcaaca cggcgtcgac
     3121 gctggtgctg tccgatgtgc gggaggccta tcccgacacg ccgttcgtcg gcacggtgcc
     3181 ggccatcaag ccggcggctg aacgcacgcg atccggcctg gtttcggtgc tggcgacgcc
     3241 gggcacggtc aagcgccaat atacgcgcga tctcatcacc caatgggccg ccaagtgcca
     3301 cgttcgcctc gtcggcagcc agtgtctggc gcggcttgcc gaaaattatg ttcgcgaggg
     3361 gttcgtcgac gaagaagccg tgcgccgcga gatcgagcct tgcttcatcg agcaggatgg
     3421 ccggcgcacc gacattgtcg tgctggcctg cacgcattat ccgttcctgg tcaaccgcat
     3481 gcgcaagact gcgccgtggc cggtcgattg gctggacccg gccgaagcga tcgcccggcg
     3541 tgccctgtcg ctgcttggcg gtcgcggcag cggggacggc gtgcatgagg g
//
LOCUS       DASYCS010000356         8096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2709323,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000356 DASYCS010000000
VERSION     DASYCS010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 8096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8096
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2709323"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1385
                     /locus_tag="VGM46_11095"
     CDS             <1..1385
                     /locus_tag="VGM46_11095"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HEY4193180.1"
                     /translation="TISVAATCLALLTLMYMRSIVVHNLKSAAAAEAEKTTFLTTDML
                     TGAMTRRYFLEALQERLDKKADTALILLDLDHFKQLNDTFGHQFGDLALSQLVATARQ
                     VFDDGTMGRLGGDEFGIFVEHADEMTILRDLRRLLKTLQAGKPYEGKIIPLSFSAGVA
                     LAPKHGSRSSDLMLLADLALYESKAKGRGRATLFNEEMQSDQRYRRLVERELRAAVYL
                     NELVLHYQPIVNADGSTYALEGLIRWRHSVRGIISPAEFIPIAERSTLIDMVGEWVFK
                     RACADIVRFPGKRISINVSGEQLKRDAVVTMFERLLRETGRSASQFVLEITETVATAA
                     SPEILKRLETLRDLGFRIALDDFGTGHCGFNYLKTFPIDSIKIDRSYINSLATDRVAQ
                     VFVSALAQIARVQDVTIVAEGVETAEEFALARAAGCTRFQGYYISRPAPHEQLRRLDF
                     NLEPKLPLSA"
     gene            1524..3575
                     /locus_tag="VGM46_11100"
     CDS             1524..3575
                     /locus_tag="VGM46_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013530081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEY4193181.1"
                     /translation="MEYRAGGRRSGPKCIAIVGPFSSGKTTLLEAILARTGAIARQNP
                     VASGNTVSDHSAEARAHAMSVEASFATTEFMGDSLTFVDCPGSVEFSFEADPVLAACD
                     LAVVVAEADEKKIPALQLIMRKLDGLKVPRILFLNKVDKAVAGVRDTLKMLQPASAAP
                     LLLRQVPLRKDGVVIGSIDLALERAYIYREYAASEIAEIPGDDKARELEARFSMLETL
                     ADHDDELMEQLLEEIEPAKDKIFDDLSADLRDGAITPVLIGAAEKGNGVLRLLKTIRH
                     DAPDVETTRQRLGAPDGNAMVAQVMKTIHTPHTGKLSVARILSGEIADGAELALPDGS
                     SARVSGIYKMFGKDQAKLPSARAGDTVALGKLEGARTGQTLTTAKGGIAALIELAPPQ
                     PVFAFALRPKERKDDVKMSAAIQRLAEEDPSLSLHHNQDSAETVLSGHGEMHLRVVRE
                     RLEGRNQIAVEGFQPAIAYRETIRKSVQQRGRHKKQSGGHGQFGDVLMEIKPLPRGSG
                     FQFTDTITGGAVPRTYIQSVETGIRDYLKSGPLGFPVADVAVNLSDGSYHAIDSSDMA
                     FQMAAKLAMKEGMAQCSPVLLEPVMKVEIFTPSEATARIIALVPQRRGQILGYDARAG
                     WDGWDVVEATMPQAEIGDLIIELRSATAGVATYRSSFDHMAELTGRLADEALNTSGKA
                     A"
     gene            complement(4275..5171)
                     /locus_tag="VGM46_11105"
     CDS             complement(4275..5171)
                     /locus_tag="VGM46_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008877602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY4193182.1"
                     /translation="MALDWDKLRVFHAAAEAGSFTHAAEVLRLSQSAISRQVSALEHD
                     VGVPLFNRHARGLVLTEQGELLYRTAHDVLMQLENVKNQLTETTDTPSGTLRVTTTVG
                     LGTGWLTERSKEFLDLHPEIELQLVLTNEELDLTMRQADCAVRLRQPQQPDLIQRRLF
                     TVHMHVFASQIYLDRHGVPKSVADLDKHRIVTFGEPVPPYLAGLNVLETIGRPAGFKR
                     GSALQINDLMSVRRAVRDGVGIAMLPDYMAHFRSGLVPVLPELEGPSFDTFFVYPDAM
                     KNQAKLKVFRDFMFTKARNWTF"
     gene            complement(5222..6196)
                     /gene="trxB"
                     /locus_tag="VGM46_11110"
     CDS             complement(5222..6196)
                     /gene="trxB"
                     /locus_tag="VGM46_11110"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEY4193183.1"
                     /translation="MNTKHAPVLIIGSGPAGYTAAVYAARALLKPVLVAGLQQGGQLM
                     ITADVENYPGFAEPIQGPWLMEQMLKQAEHVGAEIVNDIITEVDLKVRPFRAKGDSGT
                     VYTADALIIATGAQAKWLGIPTEQTFKGFGVSACAICDGFFYRGKDVAVVGGGNSAVE
                     EALYLSNLAKSVTVIHRRSDFRAERILRERLLAKANVRVIWDTVVDEVTGRPGTAPLP
                     PSVEGLKLRHASTGEETHLKVDGVFVAIGHAPAVELFVGKLKQKPNGYLWTEAGSTRT
                     DVPGVFAAGDVADDIYRQAVTAAGLGCMAALEAEKYLAGIEVHREAAE"
     gene            6424..6915
                     /locus_tag="VGM46_11115"
     CDS             6424..6915
                     /locus_tag="VGM46_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY4193184.1"
                     /translation="MPLKAELDAIDWKILRELQADGRMTNVELSSRVGISAPPCLRRV
                     KRLEETGIIRGYRALLNAPALGMDVVAFCFVGLHHQADAELKVFAERTRGWPIVRDAW
                     MVSGESDFLLHCVASDLSTFQSFVIEVLTSAPNVDTVRTALTIRQVKDEGMVEIPTAP
                     PNR"
     gene            complement(6921..7493)
                     /locus_tag="VGM46_11120"
     CDS             complement(6921..7493)
                     /locus_tag="VGM46_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008877606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEY4193185.1"
                     /translation="MSALNDYLTRSAEAIAAMVARDLTGEMERAVAAVVEALRHGKAL
                     LICGNGGSASDAIHIAGELVGRFLKERKAYNVIALPANAAVLTAWGNDYGFDTVFSRQ
                     VEAHGGPGAVLLAISTSGNSPSILAAAEQARMMGMRVISMTGDTGGKLASLSDILLNV
                     PSASTPIIQQGHLCLYHHLCEVVEARLCEG"
     gene            complement(7513..>8096)
                     /gene="rfaE2"
                     /locus_tag="VGM46_11125"
     CDS             complement(7513..>8096)
                     /gene="rfaE2"
                     /locus_tag="VGM46_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013530073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HEY4193186.1"
                     /translation="AAGVVVGKRGTARLTAEELLGALLRSHGPVVHKDAVLDADAAMR
                     MVAAWRQEGLSVGFTNGCFDILHAGHVSLLHGARAQCDRLVLGLNSDASVRRLKGEGR
                     PVNNEHDRACVLAALASVDAVIVFEEDTPLKLIEALKPDILVKGADYTVDKVVGADVV
                     QKAGGRVVLIDLVAGKSTTGTIGKLRAKGGNDN"
BASE COUNT         1569 a         2627 c         2445 g         1455 t
ORIGIN      
        1 tgaccatatc ggtcgccgca acctgcctgg cgctgttgac gctgatgtat atgcgcagca
       61 tcgtggtcca caatttgaaa tccgcagctg ccgctgaagc cgagaaaacc acgttcctca
      121 ccaccgatat gctgaccggc gcgatgacac ggcgctattt cctcgaagcg ctgcaagagc
      181 ggctggacaa aaaggccgat acggcgctga tcctgctcga tctcgaccat ttcaagcagc
      241 tcaatgatac gttcggccac cagttcggcg accttgccct ctcgcagctt gtagccacgg
      301 cgcggcaggt attcgacgac ggcacgatgg gcaggctggg cggcgacgag ttcggcattt
      361 tcgtcgagca cgccgacgag atgaccatcc tgcgcgacct gcgcagattg ctgaagacgc
      421 tccaggccgg aaagccctat gagggcaaaa tcatcccgct cagtttctcg gccggcgtgg
      481 cgctggcccc gaaacacggt tcccgttcgt cggacctgat gctgctggcc gaccttgcgc
      541 tctacgaaag caaggccaag ggccgcgggc gcgccacttt gttcaacgag gaaatgcagt
      601 ccgaccagcg ctaccgccgc cttgtcgagc gggagttgcg ggccgccgta tatttgaacg
      661 aactggtgct gcactatcag cccatcgtca acgccgatgg ctcgacctat gcgctggagg
      721 ggcttatccg ctggcggcat tcggtgcgcg gcattatatc gccggccgag ttcatcccca
      781 tcgccgaacg ctcgacgctg atcgacatgg tcggcgaatg ggtgttcaag cgcgcctgcg
      841 ccgacatcgt ccgcttcccc ggcaagcgca tcagcatcaa cgtctcgggc gaacagctga
      901 aacgcgatgc cgtggtgacc atgttcgagc ggctgctgcg cgaaaccggg cgctcggcat
      961 cgcagttcgt cctcgaaatt accgagaccg tggccacagc ggcttcgccg gaaatactga
     1021 agcggctgga aacgttgcgc gacctcggct ttcgcatcgc gcttgacgat ttcggcaccg
     1081 gccattgcgg cttcaactat ttgaagacct tcccgatcga cagcatcaag atcgaccgca
     1141 gctacatcaa cagccttgcc accgaccgcg tcgcgcaggt tttcgtttcg gcgctggcgc
     1201 agattgcgcg ggtgcaggat gtcaccatcg tcgccgaggg cgtggagacg gcggaggagt
     1261 ttgccctggc cagagccgcc ggctgcaccc gcttccaggg ctactatatc agccgcccgg
     1321 ccccgcacga gcaattgcgc aggctggact tcaaccttga gccgaaatta cccctttcgg
     1381 catgactggc cgccgtggct tatttccctt gccctccccg taaacaagtg tgacgatagc
     1441 cgtaaggcag ggaataaaat ctcgcgacgg tcgaacgggc cggccgccgc tccttggggt
     1501 taacgcccga gcggaggtca gtcatggagt atcgcgccgg aggaagacgc tcggggccga
     1561 aatgcatcgc cattgtcggt cccttttcaa gcggcaagac cacccttctc gaggccatcc
     1621 tcgcccgcac cggcgcaatt gcccgccaga atccggttgc ttccggcaat accgtatccg
     1681 accactcggc cgaagcccgc gcccacgcca tgagcgtaga ggccagtttc gctaccaccg
     1741 aattcatggg cgacagcctg acctttgtcg actgtcccgg atcggtcgag ttctcgttcg
     1801 aagcagaccc cgtgctcgcc gcctgcgacc tcgccgtggt cgttgccgag gcggacgaga
     1861 aaaagatccc cgcacttcaa ctcatcatgc gcaagcttga cggtctgaaa gtgccgcgca
     1921 tcctgtttct caataaggtc gacaaggccg ttgccggcgt gcgcgatacg ctgaaaatgc
     1981 tgcaaccggc aagcgccgcg ccgctgctgc tgcggcaggt gccgttgcgc aaggatggcg
     2041 tcgtcattgg atcgatcgat ctggcgctgg agcgcgccta catctaccgc gaatatgcgg
     2101 caagcgagat cgccgaaatt ccgggcgacg acaaggcgcg tgaactggag gcgcgcttct
     2161 cgatgctgga aacgctggcc gaccatgacg acgagttgat ggaacagttg ctggaagaga
     2221 ttgagcctgc caaagacaag attttcgacg acctttccgc cgacctgcgt gacggcgcga
     2281 taacgccggt gctgattggc gcggccgaga agggaaacgg cgtgctgcgc ctgctgaaga
     2341 cgatccgcca cgatgcgccc gatgtggaaa cgacccgcca gcgccttggc gcgcccgatg
     2401 gcaacgcaat ggtcgcacag gtgatgaaga ccatccacac cccgcatacg ggcaaacttt
     2461 ctgttgcgcg catcctgtct ggggaaatcg ccgacggcgc cgaattggcc ctgcccgacg
     2521 gcagttcggc aagagtatcc ggcatctata aaatgttcgg caaggatcag gcgaaactgc
     2581 cctccgccag ggccggcgac acggtggcgc tcggcaagct ggaaggcgcg cggaccggcc
     2641 agacactgac aacggccaag ggcggcatcg ccgcgctgat cgaacttgcg ccgccgcagc
     2701 cggtatttgc ctttgcgctg cgccccaagg agcgcaagga cgatgtgaaa atgtcggccg
     2761 ccatccagcg cctcgccgag gaagacccct cgctcagcct gcaccacaac caggattccg
     2821 ccgaaacggt tctttccggt cacggcgaaa tgcatctgcg cgtggtgcgc gaacggctgg
     2881 agggtcgcaa tcagattgcg gtcgaaggct ttcagcccgc cattgcctac cgcgaaacga
     2941 tccgcaagtc ggtgcagcag cgcggacgcc acaagaagca gtccggcggt cacggccagt
     3001 tcggcgacgt gttgatggag atcaagccgc tgccgcgcgg ctccggcttt cagttcaccg
     3061 acacgatcac cggcggcgcg gtgcccagga cctatatcca gtcggtggaa accggcattc
     3121 gcgattatct gaaatccggc ccgctcggtt ttccggtcgc cgatgtcgcc gtcaaccttt
     3181 ccgatggttc ctatcacgcg atcgattcct ccgacatggc tttccagatg gcggcgaagc
     3241 tcgccatgaa ggaaggcatg gcgcaatgct cgccggtgct gctcgaaccg gtgatgaagg
     3301 tcgagatatt cacgccttcg gaagccaccg ccaggatcat cgcgctggtc ccgcaacggc
     3361 gcgggcaaat cctcggctac gacgcgcgcg ccggctggga tggctgggac gtggtggaag
     3421 cgacgatgcc gcaagccgag atcggcgacc tgatcatcga attgcgctcg gcgaccgctg
     3481 gcgtggcgac atatcgctca tccttcgacc atatggccga actgaccggc aggcttgccg
     3541 acgaggcact gaacaccagt ggcaaagctg cctgaccgct aaagccgccg cctggaatgg
     3601 ttgaaataag acggcgcccg aactgatcgg acgccgtctg tctgcggacc gcttcccgcg
     3661 ggaggtaaac ggcaggtccg ccgcacgatc acgccggcgc atcacccgcc ggcgacggaa
     3721 aaaggctgga cgcgaccgct gcctgagccc gaacccttcg agggatgccc ccatctcccg
     3781 aaggactcat gccgcgtcct attgtctgac taatacaaac ttcgcgcagg ctaaacgtta
     3841 cgcgggttac acgtcaccgt tacgccggcc gaaaccgctt ctgccggcga aaatgtccga
     3901 aatttttcgg cgatgaacgg gaatttttgg caacaaaaaa gccggatcga gagatccggc
     3961 tgagttgaat ggaagcgccc gttagggcta aacctccaga ggggaacact cgaggacgct
     4021 aatgggagga gaacgcgtcc aggagatgcg ttatatatcg ccatacactg cccaatattc
     4081 aagcacctat tttgcaacgc acgcatgcaa aaagggattg tctgtggtcc aattgccaat
     4141 tggtgaccat aggaccagat atggctgaaa accgatgaaa ctggcggttt tcatgcaagg
     4201 tcggccgcct tccggctatc ggcgccctgt tccggtacgc agaataccgc gctgccgcac
     4261 cgccggccgc tatctcaaaa cgtccagttg cgcgccttgg tgaacatgaa atcgcgaaac
     4321 accttgagct ttgcctgatt cttcatcgca tcgggataga cgaagaacgt atcgaaggaa
     4381 ggcccttcga gttcgggcag gacaggcacc agcccagacc ggaaatgcgc catgtagtcc
     4441 ggcagcatgg cgatgccgac gccatcccgc accgccctgc gcaccgacat cagatcgttg
     4501 atctgcaacg ccgagcctcg cttgaagccg gctggccggc cgatggtttc gagcacgttt
     4561 agccccgcga gatagggcgg caccggctcg ccaaaggtaa cgatgcggtg cttgtcgaga
     4621 tcggccaccg acttcggcac gccgtggcgg tcgagataga tttgcgaggc aaagacgtgc
     4681 atgtgcacgg tgaacagccg gcgctggata aggtcgggct gctgcggctg acgaagccgc
     4741 accgcgcaat cggcctggcg catggtgagg tcgagttcct cattggtcaa aaccagctga
     4801 agctctattt cggggtgaag gtcgaggaac tccttcgaac gctcggtcag ccagccggtc
     4861 cccaggccca cggtcgtcgt cacgcgcagc gtgccggaag gcgtgtcggt cgtctcggta
     4921 agctggttct tgacgttttc cagttgcatc agcacgtcat gcgctgtgcg gtagagcagt
     4981 tcgccctgct cggtcagcac cagtccgcgc gcgtgccggt tgaacagcgg cacgccgaca
     5041 tcatgctcca gcgcgctgac ctggcgcgag atggccgact gcgacagccg cagaacctcg
     5101 gccgcatgcg tgaaggaccc agcctccgcc gccgcgtgaa acacgcgaag cttgtcccaa
     5161 tccaacgcca tggcttcccc tcttgttcgg cgtctctacg gcaaatcagg tttttgaacg
     5221 gctattccgc tgcttctctg tgaacctcta tgcctgccag gtatttttct gcttccagtg
     5281 ccgccatgca gccgagaccc gccgcggtca ctgcctggcg gtagatatcg tcggcaacgt
     5341 cgccggcggc gaaaacgccc ggcacgtccg ttcgtgtcga gcctgcttcg gtccacagat
     5401 aaccattcgg cttctgcttc agcttgccga cgaaaagttc gacggccggc gcatggccga
     5461 tggcgacgaa cacgccatcc accttgagat gggtttcctc gccggtcgaa gcgtgccgaa
     5521 gcttcagccc ttccaccgaa ggcggcagcg gcgccgtgcc cggccgcccg gtgacctcgt
     5581 cgacgaccgt atcccagatg acgcggacat tggccttggc aagcagccgc tcgcgcagga
     5641 tgcgctcggc gcggaaatcg ctgcgccggt ggatgaccgt cacgcttttg gcgaggtttg
     5701 acagataaag cgcttcttcc accgccgaat tgccgccacc cactaccgcg acatccttgc
     5761 cgcgatagaa gaagccatcg cagatggcgc aggccgatac gccgaagccc ttgaaggtct
     5821 gctcggtggg aatgcccagc cacttcgcct gcgcgccggt ggcgatgatg agcgcgtccg
     5881 cggtataaac cgtgcctgaa tcgcccttgg cgcggaacgg ccgcaccttg aggtcgacct
     5941 cggtgatgat gtcgttgacg atctcggcgc cgacatgctc ggcctgcttc agcatctgct
     6001 ccatcagcca tggcccctgg atcggctcgg caaaaccagg ataattttcc acatcggcgg
     6061 tgatcatcaa ctgtccgccc tgctgcaacc cagccaccag caccggcttg agcaaggcgc
     6121 gggcggcata gacggctgct gtatagccgg ccgggccgga accgatgatg agaactggcg
     6181 cgtgcttggt attcatggac gacctctgcg gcagccgcga agcgcggcaa aaaaatatat
     6241 tcagctgttc cttgcaatgt atgtgctgcc cgccgccctg ccaagaaggc caggcgcagt
     6301 gtccccggaa agctgcgagc ttcgtaaacg cgaccgatag cggtggaaac gctctggcgc
     6361 gcgcccaaaa ctcgtttaag aaacagctat gcaactgtcc ttcgcatgaa acgaaagcac
     6421 gccatgccct tgaaggccga actcgacgcc atcgactgga agatcctgcg cgagcttcag
     6481 gcggatgggc gcatgaccaa tgtggaattg tcgagccggg tcggcatctc cgcgccgccc
     6541 tgcctgcggc gcgtcaagcg cctggaggaa accggcatca ttcgcggcta ccgcgccctt
     6601 ctcaacgcgc cggcgctggg catggatgtg gtggcgttct gtttcgtcgg cctccaccac
     6661 caggcggacg cggaattgaa ggtctttgcc gagcgcacgc gcggctggcc gatcgttcgc
     6721 gacgcctgga tggtgtccgg cgaatcagac ttcctgcttc attgcgtggc aagcgacctt
     6781 tccaccttcc agagcttcgt catcgaagtc ctcacctccg ccccgaacgt cgatacggtg
     6841 cgcaccgcgc tcaccatccg ccaagtcaag gacgaaggca tggtggaaat tccgaccgcg
     6901 ccgcccaatc gctgaggaaa tcacccttcg cagagccgcg cttcgaccac ttcgcacaga
     6961 tgatggtaga ggcacaggtg gccctgctgg atgatcggcg tcgaggccga gggcacgttg
     7021 agcaggatgt ccgacagcga cgccaacttg ccgccggtat cgccggtcat ggaaatcacc
     7081 ctcatgccca tcatccgcgc ctgttcggcg gcggcaagaa tgcttggcga attgccgctg
     7141 gtcgagatcg ccagcagcac cgcgcccggc ccgccatgcg cctcgacctg ccgggaaaac
     7201 accgtgtcga agccgtaatc attgccccac gcggtcagca ccgccgcatt ggccggcagc
     7261 gcgatgacat tataggcctt gcgctccttc aggaagcggc cgaccagttc gccggcgata
     7321 tggatggcgt cgcttgccga gccgccattg ccgcagatga gcagcgcctt gccatgccgc
     7381 aacgcctcga cgacggcggc gacggcgcgc tccatttcgc cggtcaggtc gcgcgcgacc
     7441 attgccgcga tcgcttccgc cgagcgggtg agatagtcgt tcagtgcgct catgcggcct
     7501 ccgattgcgc cttcaattgt cgttgccgcc cttggcgcgc agcttgccga tggtgccggt
     7561 cgtgcttttt ccggccacca gatcgatcag cacgacgcgc ccgccggcct tttgcaccac
     7621 gtccgcgccg accaccttgt cgaccgtata atccgcgccc ttgaccagaa tatccggctt
     7681 caacgcctcg atcagcttga gcggcgtatc ctcctcgaac acgatgacgg cgtcgacgga
     7741 ggcgagcgct gcaagaacgc aggcgcggtc atgctcgttg ttgaccgggc ggccctcgcc
     7801 cttcagcctt cgcaccgaag cgtcgctgtt caggcccagc accagccggt cgcattgtgc
     7861 gcgcgcgccg tgcagcaggc tgacatgacc ggcatgcaat atatcgaaac agccattggt
     7921 gaagccgacg ctcagcccct cctgtcgcca ggccgccacc atgcgcatgg cggcgtcggc
     7981 atcgagaacc gcatccttgt gaacgaccgg cccatgcgag cgcaaaagcg cgcccagcaa
     8041 ttcctcggcg gtgagcctcg cggtgccgcg cttgcccacc accacgccgg ccgccg
//
LOCUS       DASYCS010000357        10480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1985073,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000357 DASYCS010000000
VERSION     DASYCS010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 10480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10480
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1985073"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VGM46_11130"
     CDS             <1..333
                     /locus_tag="VGM46_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006325581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY4193187.1"
                     /translation="AQRRDLAEQGYFEEPSDYSAEPYIYTRALIEDGRDNRVMTGPID
                     THCPVHILQGLADPDVPPSHALKLVGLLPSDDVTLSLIPGGDHRLSRPQDLDMLVRSV
                     DALVRRPA"
     gene            334..1476
                     /locus_tag="VGM46_11135"
     CDS             334..1476
                     /locus_tag="VGM46_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HEY4193188.1"
                     /translation="MRISIPVSAFVAAIVGFGGTLAIIIAAADAVGATQLQTASWVTA
                     LCLAMAAETAWLSWRTKMPVITAWSTPGAALIAASTGFSMGEAVGAFLVTGLLLVITG
                     LFKPLTRLIAKIPGSVASGMLAGIVVTFAIGAVKAIPSDPWLILPLIAAFFVIRLFNP
                     ALSVLAVLIGGGIAAFLTGRVGGLPAPELSTLTLIAPQFTLQAIIGLAIPLYLVTMAS
                     QNLSGLAVLKADGYEPAPGPLIGVTGLGSILTAPFGASTTNLAAISAAICTGPDVHPD
                     PGQRWKTGPFYALAYLIFAAFGASLVAIFAVLPQSLIVLVAGLALLASLANALSIALK
                     DPQERMAATITFAVTASGLTLFGIGSAFWGLIGGLLVLMLDRAKKK"
     gene            1593..2039
                     /locus_tag="VGM46_11140"
     CDS             1593..2039
                     /locus_tag="VGM46_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018430565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2852 domain-containing protein"
                     /protein_id="HEY4193189.1"
                     /translation="MNTSALIRPAWTPATIALMVVGFMVFWPLGFAMLAYIIWGDRLD
                     GFKRDVNRATDGIFAGCRRGSEKAARWGNRGGAARTGNVAFDDWREKELERLNEERRK
                     LDEMLDQFDEYARELRRAKDQDEFDRFMANRNKSTAPTTTPKPGGD"
     gene            2136..2894
                     /locus_tag="VGM46_11145"
     CDS             2136..2894
                     /locus_tag="VGM46_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006325590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEY4193190.1"
                     /translation="MSFGFFRTLSKISAPPMEAREHTVAGRTLPLRIVENERARRLTL
                     RIDAGGQGLRITVPPGLRRGEVEKFLHRHQGWLEQRLAKVPDRPQVRPGIKIPFRGVP
                     HLIVHEPGRRGTVTVDDEGGGPSLVVHGERLHLPRRVSDFLKREARREIEALVTKHTA
                     TVGRAAKAIRLRDTSSRWGSCTSDGTLSFSWRIMMAPPPVINYLVAHEVAHLKEMNHG
                     PKFWKLCEELCPDTERCKAWLKRNGSALQAIQFE"
     gene            complement(2941..4029)
                     /locus_tag="VGM46_11150"
     CDS             complement(2941..4029)
                     /locus_tag="VGM46_11150"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019863145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2'-deoxycytidine 5'-triphosphate deaminase"
                     /protein_id="HEY4193191.1"
                     /translation="MRKTGILPDQDIAALFQSGALKSERALDGDQIQPASLDLRLGQT
                     AWRVRASFLPGPDHTVADKLDRLKLHEISLADGAVLETGCVYIVPLMESLALPGDISA
                     SANPKSSTGRLDIFTRVMTDNGHEFDKIAAGYHGPLYLEVSPRTFPIVVRGGSRLSQI
                     RFRTGNALLSEQELQALHRSETLVATDPPNISGGGIALSIDLAGDENGLVGYRGKHHT
                     GLVDVDRRGAFDAIDFWEPLHKRRDGELVLDPNEFYILVSREAVHVPPLYAAEMTPFD
                     PLVGEFRVHYAGFFDPGFGHSAAGGTGSRAVLEVRSHEVPFILDHGQIVGRLVYEHMK
                     ERPKALYGTDLGSNYQAQGLKLSKHFRA"
     regulatory      4139..4216
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            4271..5461
                     /locus_tag="VGM46_11155"
     CDS             4271..5461
                     /locus_tag="VGM46_11155"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HEY4193192.1"
                     /translation="MTTQEKRNWKPATQLVHSGTLRSQFGEMSEALFLTQGYVYPTAE
                     AAEARFKGEDPGFIYSRYANPTVDMFEKRMCALEGAEDARATASGMAAVAAAILCGLK
                     AGDHIVSARAIFGSCRWIVETLAPHYGIEATLVDGREIANWEAAVKPNTKLFFLESPT
                     NPTLEVVDIAAVAALATSVGARLVVDNVFATPLQQKPLELGAHVVVYSATKHIDGQGR
                     CLGGIILSDKDWIDENLHNYFRHTGPSLSPFNAWTLLKGLETLPIRVAHQTQSAGKIA
                     DFLAEKRQVARVVYPGRADHPQADIVKKQMAGGSTLICLDLKGGKEAAFAFQNALDII
                     LISNNLGDAKSLITHPATTTHKNLSEEARAELGIGPGTLRFSTGLEDIDDLLADIDQA
                     LAKA"
     gene            5458..5865
                     /gene="apaG"
                     /locus_tag="VGM46_11160"
     CDS             5458..5865
                     /gene="apaG"
                     /locus_tag="VGM46_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006199938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HEY4193193.1"
                     /translation="MTARNRYSAVTGGIEVQVEPFYLEDRSDPAQGRYVWGYRVTIDN
                     QSDGLVQLLSRYWQITDGNGRTEEVRGDGVVGDQPELNPGDSYQYTSGCPLPTPSGIM
                     VGHYTMLGSMGETFDIAIPAFSLDMPGSRRTVN"
     gene            complement(5888..6880)
                     /locus_tag="VGM46_11165"
     CDS             complement(5888..6880)
                     /locus_tag="VGM46_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006199940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone"
                     /protein_id="HEY4193194.1"
                     /translation="MLEALEVTQHTPKLGEFGYAGDDHVVPFEVDALDARGRTVQLGA
                     MLDTILSRHDYPEPVARLLAEACVLAVLLGTSLKFEGKFILQTRTDGPVDMLVADFST
                     PHALRAYARFDADRLEALTAAGESSQQTLLGNGILALTIDQGEHTKRYQGIVALDGVT
                     LEEAARTYFRQSEQIPTELKLSVAKLVAPGGHEQWRAGGILAQFLPESSERMRVADLP
                     GGDGDETEIPVDDAWQEVLALLGTIEPTELIDPTVGAERLLYRLFHEHGVRAFDGVRV
                     ADRCSCSKDKIRGILEGFSAEEIRESTENGKISVACEFCSTEYQFDPAEFNPAK"
     gene            complement(6984..7895)
                     /gene="argF"
                     /locus_tag="VGM46_11170"
     CDS             complement(6984..7895)
                     /gene="argF"
                     /locus_tag="VGM46_11170"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEY4193195.1"
                     /translation="MSLRHFSDLSTVSESDLRAMLDDAAARKKRLKAGERSKPLDGKV
                     LAMIFDKPSTRTRVSFDVGMRQLGGETIMLTGTEMQLGRSETIADTAKVLSRYVDAIM
                     IRTTSHDRMVELTQNATVPVVNGLTDDTHPCQIMADILTFEEHRGPVAGKTFTWTGDG
                     NNVLHSLLEASARLRFNLNIAVPEGSEPMQKYVDWSKANGGAVRFFGSADEAVRGADC
                     IVTDSWVSMGQEHRARGNNVFLPYQVNAALMAKANPDALFMHCLPAHRGEEVTDEVMD
                     GSQSVVFDEAENRLHAQKAILAWCLGA"
     gene            complement(7912..9108)
                     /locus_tag="VGM46_11175"
     CDS             complement(7912..9108)
                     /locus_tag="VGM46_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019863139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY4193196.1"
                     /translation="MSGSAIFETFARAPLAFDHGEGAWLVTTGGERYLDFAAGIAVNS
                     LGHGHPHLVAALTEQAAKLWHVSNLYEIPAQTRLAERLVEATFADKVFFTNSGAEALE
                     CAIKTARRYHYVSGQPERFRIITLEGAFHGRTLATIAAGGQQKYLEGYGPKVEGFDQV
                     PYDDLAAVEQAITPETAAILVEPVQGEGGIRTVSTQTLKRLRELCDEHGLLLIFDEVQ
                     SGVGRTGKLFAHEWSGIAPDLMAIAKGIGGGFPMGACLATDEAARGMTVGVHGTTFGG
                     NPLAMAVGNAVLDVVLEDGFLEEVQRKALLMKQGLAQVADEFPGVIEDIRGTGLMLGL
                     KCAMSNTDVNMALRGQHLLAVPAGDNVIRLLPPLTVTDAEINEALERIRAAAGSLAGA
                     AAVAAQ"
     gene            9473..9991
                     /locus_tag="VGM46_11180"
     CDS             9473..9991
                     /locus_tag="VGM46_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcrA family cell cycle regulator"
                     /protein_id="HEY4193197.1"
                     /translation="MNWTDERVELLKKLWSEGLSASQIAAQLGGVSRNAVIGKVHRLK
                     LSSRGRATAAPARQKKTAQPGAVKSVSRAATTVRHMTSSIGATALQTQFDAEPAARIY
                     MRPATENVVVPISRHLHLVELTERTCKWPNGDPLSEDFHFCGNDAAETGPYCKYHARL
                     AFQPASERRRSR"
     gene            complement(10090..>10480)
                     /locus_tag="VGM46_11185"
     CDS             complement(10090..>10480)
                     /locus_tag="VGM46_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HEY4193198.1"
                     /translation="NAALPLVEVCRRLTSSDLPQIHAAMHPLFRDVTDHIRTVQEKID
                     SLREVLAFAFEASLLVGQSQETAISKKLASWAAILAVPTAFAGIYGMNFTDMPELKME
                     YGYPAVLAAIAAICCFLYWRFRKNGWL"
BASE COUNT         1875 a         3362 c         3339 g         1904 t
ORIGIN      
        1 gcgcaaaggc gcgacctggc cgagcagggc tatttcgagg aaccgtccga ctattcggcc
       61 gagccttata tctacacgcg cgccttgatc gaggacggcc gcgacaaccg ggtgatgacc
      121 gggccgatcg acacgcattg cccggtgcat atcctgcaag ggctggccga tccggacgtt
      181 ccgcccagcc atgcgctgaa gctggtcggc ctgctgccgt ccgacgacgt gacgctgtcg
      241 ctcattcccg gcggcgacca tcgcctgtcg cggccacagg acctcgacat gctggtgcgt
      301 tccgtcgatg cgctggtgcg gaggcccgca taaatgcgca tttccattcc cgtatcggcc
      361 ttcgtcgcgg ccattgtcgg ttttggcggc acgctggcca tcatcatcgc ggcggccgac
      421 gccgtcggcg cgacgcaact gcaaaccgca agctgggtga cggcgctctg cctggcgatg
      481 gcggcggaga cggcatggct ttcatggcgt accaagatgc cggtcatcac cgcatggtcg
      541 acgccgggcg cggcgctgat cgccgccagc accggatttt cgatgggcga ggcggtggga
      601 gccttcctcg tcaccggcct gcttctggtc atcaccgggc tgttcaagcc gctgacgcgc
      661 ctgatcgcca aaataccagg ctcggtggca tcgggcatgc tggccggcat cgtcgtcacc
      721 ttcgccattg gcgcggtcaa ggctatcccg tccgatccgt ggctgatcct gccgctgatc
      781 gccgccttct tcgtcatccg cctgttcaat ccggcgctgt cggtgctggc ggtgctgatc
      841 ggcggcggca tcgccgcctt tctgaccggg cgcgtcggcg gtctgcctgc gcccgagctt
      901 tcgacgctga cgctgattgc gccgcagttc acgcttcagg ccatcatcgg gctggccatt
      961 ccgctctacc tcgtcaccat ggcctcgcag aacctgtccg gcctcgccgt gctgaaggcg
     1021 gacggctatg aacccgcacc cggcccgctg atcggcgtta ccgggctggg atcgatattg
     1081 accgcgccgt tcggggcctc gaccaccaac ctcgccgcga tctcggcggc gatctgcacg
     1141 gggccggacg tgcatcccga ccccggccag cgctggaaga cagggccgtt ctacgcgctt
     1201 gcctatctga tcttcgccgc gtttggcgct tccttggtgg cgatcttcgc cgtgctgccg
     1261 caaagcctga tcgttttggt ggccgggttg gcgctgcttg cctcgctcgc caatgcgctg
     1321 tccatcgcgc tcaaggaccc gcaagagcgg atggcggcga ccatcacctt tgccgtcacc
     1381 gcatcgggcc tgaccctgtt cggcatcggc tccgccttct ggggcctgat cggcgggctg
     1441 ctggtgctga tgctcgaccg cgcgaaaaag aaatagcgat tccagcccgt tgtccggttt
     1501 tggcggccgc tgtcttgaat cgccgttttt gtgttcccat ctcgattcca tgcagccacc
     1561 gtggctgcct ccaactgaag gaacgggaga aaatgaacac ttctgcattg atccgaccgg
     1621 catggacgcc ggcgaccatt gcgttgatgg tggttggctt catggttttc tggccgctcg
     1681 gtttcgccat gctcgcctac atcatctggg gcgatcggct tgacggtttc aagcgcgacg
     1741 tgaaccgcgc caccgacggc atcttcgccg gctgccgccg gggttcggaa aaggccgcgc
     1801 gctggggcaa tcgcggcggt gctgcccgca ccggcaacgt cgctttcgat gactggcgcg
     1861 aaaaagagct tgagcgcctg aacgaggaac ggcgcaagct ggacgagatg ctcgaccagt
     1921 tcgacgaata tgcccgcgag ttgcgccgcg ccaaggacca ggacgagttc gaccgcttca
     1981 tggccaaccg caacaagtcg accgcgccga cgacgacccc gaagccgggc ggcgactgac
     2041 ccccatgtga cgggaacctt ggaaacggcg ctgcgactgc ggcgccgttt cttttttgtt
     2101 cttcctcaat ccctcgaatc acatatcgtc cagccatgtc tttcggcttc tttcgcaccc
     2161 tgtcgaaaat ttcagcgccg cccatggagg cccgcgaaca cactgtcgcg ggccgcaccc
     2221 tgcccttgcg catcgtggag aacgagcgcg cgcgccggct gaccctgcgc atcgatgccg
     2281 gcggccaggg cttgcgcatc accgtgccgc ccggcctgcg gcgcggcgag gtcgagaaat
     2341 tcctgcaccg ccatcagggc tggctggaac agcggctggc caaggtgccg gaccgcccgc
     2401 aggtgcgccc cggcataaaa atcccgtttc gcggcgtgcc gcacctgatc gtgcacgaac
     2461 cgggccgacg cggcacggtg acggtggacg acgagggcgg cgggcccagc ctggtcgtcc
     2521 atggcgagcg cctgcatctg ccgcgccgcg tcagtgactt cctcaagcgc gaggcaaggc
     2581 gcgagatcga agctctggtg accaagcata cggcgacggt tggccgcgcc gccaaggcga
     2641 tccgcctgcg cgatacttca agccgctggg gttcatgcac ttccgacggc acgctttcct
     2701 tttcatggcg catcatgatg gcgccgccgc cggtgataaa ttacctggtg gcgcacgaag
     2761 tggcgcatct gaaagagatg aaccacggcc cgaaattctg gaagctgtgc gaggagcttt
     2821 gcccggacac ggagcgctgc aaggcgtggc tcaagcgcaa tggcagcgcg cttcaggcca
     2881 tccagttcga gtgaacgggc ggctggccgc cgcccgcgcc gggctttccc gaactcctgg
     2941 ctacgcgcga aaatgcttgg aaagcttcag gccctgcgcc tgataattgg aacctaggtc
     3001 ggtgccgtag agcgccttgg gccgctcctt catatgctca tagaccagcc gcccgacgat
     3061 ctggccgtga tccagaatga agggcacttc atggctgcgc acttccagca cggcgcggct
     3121 gccggtgccg cctgccgccg aatggccgaa gcccggatcg aagaagcccg cataatgaac
     3181 gcgaaactcg ccgacaagcg gatcgaacgg cgtcatttcg gcagcgtaga gcggcggcac
     3241 atgcaccgcc tcgcggctga cgaggatgta gaactcgttg ggatcgagca ccagttcgcc
     3301 gtcgcgcctc ttatgaagcg gctcccagaa atcgatggcg tcgaaggccc cgcgccggtc
     3361 gacatcgacc aggccggtgt gatgcttgcc gcgatagccg acaagtccgt tctcgtcgcc
     3421 ggcaagatcg atcgacagcg cgatgccgcc gcccgaaatg ttgggtggat cggtggcgac
     3481 cagcgtttcg gagcggtgca gcgcttgcag ttcctgttcc gacagcagcg cattgccggt
     3541 gcggaagcgt atctgcgaca ggcgcgaccc gccgcgcacg acgatcggga aggtgcgcgg
     3601 gctgacttcc agatagagcg ggccatggta gccggcggcg atcttgtcga actcatggcc
     3661 gttgtcggtc atgacgcgtg tgaagatgtc gagacggccg gtcgagcttt tcgggttggc
     3721 ggaggcggaa atgtcgcccg gcagcgccag gctttccatc agcggcacga tgtagacaca
     3781 gccggtttcc agcaccgcgc cgtcggcaag gctgatctcg tgcagcttca ggcggtcgag
     3841 cttgtcggcg accgtatggt cgggaccggg caggaaggaa gcgcgcacgc gccacgccgt
     3901 ctgccccagc cgcaggtcga ggctcgccgg ctggatctgg tcgccatcca gcgcacgctc
     3961 gcttttcagc gcgcccgact ggaacagcgc cgcgatatcc tgatcgggaa gaatgcctgt
     4021 cttgcgcaaa gacccactcg cagtctggct caattctttc aattggccca aatctctaat
     4081 tggcccaagt gtctaattgg cagagcggtt gacgcaagcg ggggcgagga ataaacgggc
     4141 tttatcccgt ggtgatttgg ccggtcggct tgcagccacg ttaaacaagt cgctaaagga
     4201 ccgtcggccg caaggcagta tggaccggtg gcgattttcg cgaccggttt tttgttttta
     4261 aggcacaggc atgaccaccc aagaaaaacg caactggaag cccgccaccc aactcgtgca
     4321 ttccggcacg ctgcgatcgc agttcggcga aatgtcggaa gcgctgttcc tgacgcaggg
     4381 ctatgtctat ccgaccgccg aagccgccga ggcgcgcttc aagggcgagg atccgggctt
     4441 catctattcg cgttacgcca acccgaccgt cgatatgttc gaaaagcgca tgtgcgcgct
     4501 ggaaggcgcg gaagatgcgc gcgccaccgc atcgggcatg gcggccgtcg ccgccgccat
     4561 cttgtgcggc ctgaaagccg gcgatcatat cgtttcggcg cgcgccatat tcggctcctg
     4621 ccgctggatc gtcgagacgc tggcgccgca ctacggcatc gaggccacgc tggtcgatgg
     4681 acgcgagatc gccaattggg aagcggcggt caagccgaac accaagctgt tctttctcga
     4741 aagcccaacc aatccgacgc tggaagtggt cgatatcgcg gcggtcgcgg cactggccac
     4801 ctcggtcggc gcgcggctgg tggtcgacaa tgttttcgca accccgctac agcaaaagcc
     4861 gttggaactg ggcgcgcatg tggtggtcta ttcggccacc aagcacattg acggccaggg
     4921 ccgctgcctt ggcggcatca tcttgtccga caaggactgg atcgacgaga atttgcacaa
     4981 ttatttccgt cacaccggcc ccagcctgtc gcccttcaac gcgtggacgc ttttgaaggg
     5041 gctggagacg ctgccgatcc gcgtcgcaca ccagacgcaa agcgccggca agatcgccga
     5101 tttcctggcg gaaaagcgac aagtcgcgcg ggtcgtctat ccgggccggg ccgatcaccc
     5161 gcaggccgac atcgtcaaaa agcagatggc cggcggctcg acgctgatct gcctcgacct
     5221 gaagggcggc aaggaagcgg ccttcgcgtt ccagaatgcg ctggacatca tcctgatttc
     5281 caacaatctc ggcgacgcca agagcctgat cacgcatccg gcgacgacga cgcacaagaa
     5341 cctcagcgaa gaggcgcgcg ccgaactcgg catcgggccg ggcacgctgc gcttttcgac
     5401 cgggctggaa gacattgacg acctgctcgc cgatatcgac caggccctgg ccaaggcatg
     5461 accgcgcgca accgctatag cgccgtcacc ggcggtatag aggtgcaggt cgagccgttt
     5521 tacctggagg atcgctccga tccagcacag ggccgctatg tctggggtta ccgggtcacc
     5581 atcgacaacc agtcggacgg actcgtgcag ttgctatcgc gctattggca aatcaccgat
     5641 ggcaatggcc ggaccgaaga agtgcgaggc gacggcgtcg tcggggacca gcccgagctg
     5701 aaccccggcg acagctatca atatacgtcc ggctgcccgc tgccgacgcc ctccggcatc
     5761 atggtcgggc attatacgat gctgggcagc atgggcgaga cgttcgacat cgccattccc
     5821 gccttttcgc tcgacatgcc gggatcgaga cggacggtga actgaagcca ccgcgcgtcc
     5881 ggggctatca cttggccgga ttgaattcgg ccgggtcgaa ttgatattcg gtggagcaga
     5941 actcgcaggc gacgctaatc ttgccgttct cggtgctttc gcgaatttcc tcggcggaaa
     6001 atccttcgag aatgccgcga atcttgtcct tcgagcagga acaccggtcg gccacccgca
     6061 cgccgtcgaa tgcgcgcacg ccatgttcgt ggaacaggcg gtagagcagg cgttcggcgc
     6121 cgacggtcgg gtcgatcaat tcggtcggct cgatggttcc aagcagcgcc agcacttcct
     6181 gccaggcatc gtcgaccgga atttccgtct cgtcgccatc gccgcccgga aggtcggcaa
     6241 cgcgcatgcg ctcgctcgat tccggcagga attgcgccag tatgccgccg gcgcgccatt
     6301 gctcatggcc gccgggcgcc accagcttgg cgaccgacag cttgagttcg gtcgggatct
     6361 gttccgactg gcggaaatag gtgcgcgcgg cttcttccag cgtcacgccg tcgagcgcga
     6421 cgatgccctg atagcgcttc gtatgctcgc cctggtcgat ggtcagcgcc agaatgccgt
     6481 tgccaagcaa ggtctgctgc gagctttcgc cggccgccgt caacgcttcc agccggtcgg
     6541 catcgaagcg cgcataggcg cgcagcgcat gcggcgtgga aaaatccgcg accagcatgt
     6601 cgaccggacc atccgtgcgg gtttgcagga tgaacttgcc ttcgaacttc agcgaggtgc
     6661 caagcagcac ggccagcacg caggcctcgg ccagcagcct tgcgaccggc tcggggtaat
     6721 cgtggcgcga caggatcgtg tcgagcatcg cgccgagttg gacggtgcgg ccgcgcgcgt
     6781 ccagcgcatc gacctcgaaa ggtacgacgt ggtcgtcgcc ggcatagccg aattcgccaa
     6841 gttttggggt gtgttgcgtc acttcaaggg cttccagcat gacatatctc cagccgccag
     6901 ccgcgccgtc cttgtaacgg catggtttga aggggccaat ctttgcgatg atgggccgca
     6961 gataggcatc gcgcaggcag cgttcaagcg ccgagacacc acgccaatat ggccttctgc
     7021 gcatggagac ggttttcggc ttcgtcgaac accaccgact gggatccgtc catgacctcg
     7081 tcggtcactt cctcgccgcg atgggcgggc aggcagtgca tgaacagcgc gtccgggttt
     7141 gccttggcca tcagcgccgc attgacctga tagggcagga agacgttgtt gccgcgcgcc
     7201 cggtgctcct ggcccatcga cacccagctg tcggtaacga tgcagtcggc gccacggacc
     7261 gcctcgtcgg cggagccgaa gaaccggaca gcgccgccat tggccttcga ccaatcgaca
     7321 tatttctgca tcggctcgct gccttccggc acggcgatgt tgaggttgaa acgcagccgc
     7381 gcggatgcct ccagcagcga atgcagcaca ttgttgccgt cgcccgtcca ggtgaaggtt
     7441 ttgcccgcca ccgggccgcg atgttcctcg aaagtcagta tatcggccat gatctggcag
     7501 ggatgggtat cgtcggtcag gccgttgacg acagggaccg tggcattctg cgtcagctcg
     7561 accatgcggt cgtgcgaggt ggtgcggatc atgatggcat cgacgtagcg cgacagcact
     7621 ttggcggtat cggcgatggt ctcggagcgg ccaagctgca tttccgtgcc ggtcagcatg
     7681 atggtttcgc cgcccagctg ccgcatgccg acatcgaagg aaacgcgcgt ccgcgtcgaa
     7741 ggcttgtcga agatcatcgc caagaccttg ccgtccagcg gcttcgaccg ctcgccagcc
     7801 ttgaggcgct tcttgcgggc cgccgcgtcg tccagcatgg cgcgcagatc gctttccgaa
     7861 acggtggaaa ggtcggagaa atgacgaagg ctcatcaaaa aatttccgga tttactgggc
     7921 ggcgacggcc gcagccccgg cgaggctgcc ggcggcggcg cggatacgct ccagcgcctc
     7981 gttgatctcg gcgtcggtaa cggtcagcgg cggcaaaagg cggatgacgt tgtcgcccgc
     8041 cggaacggcc agcagatgct ggccgcgcag cgccatgttc acatccgtgt tcgacatcgc
     8101 acatttgagg ccgagcatca ggcccgtgcc gcggatatcc tcgatgacgc cgggaaattc
     8161 gtcggcaacc tgcgccaatc cctgcttcat caacagcgcc ttgcgctgca cctcttcgag
     8221 gaagccgtct tccagcacca cgtcgagcac ggcgttgccg accgccatgg ccagcggatt
     8281 gccgccgaaa gtggtgccgt ggacgccgac ggtcatgccg cgcgccgcct cgtccgtggc
     8341 aaggcaggcg cccatgggaa agccgccgcc gatgcccttg gcgatcgcca tcaggtccgg
     8401 ggcaatgccc gaccattcat gggcaaacag cttgccggta cggccaacgc cgctctgcac
     8461 ttcgtcgaag atcagcagca ggccgtgctc gtcgcacaat tcgcgcagcc gcttcagcgt
     8521 ctgtgtcgaa acggtgcgga tgccgccctc accctgcacg ggttccacca ggatggcggc
     8581 ggtttccggc gtgatcgcct gctcgacggc agcgaggtcg tcatagggaa cctggtcgaa
     8641 gccttcgacc ttggggccat agccctcaag atatttctgc tggccgccgg cggcgatggt
     8701 ggccagcgtg cggccatgga aggcgccttc cagggtgatg atgcggaacc gctccggttg
     8761 gccgctcaca tagtggtagc gccgcgccgt cttgatggcg cattccagcg cctcggcgcc
     8821 cgaattggtg aagaaaacct tgtcggcgaa ggtcgcctcg acaagccgct cggccaatcg
     8881 ggtctgcgct ggaatctcat aaaggttcga aacgtgccac agcttggccg cctgttcggt
     8941 cagggccgcg accaggtgcg gatggccgtg gccgagcgaa ttgacggcga tgccggcggc
     9001 gaaatcgagg tatcgctcgc cgcccgtcgt gaccagccag gcgccctcac cgtggtcgaa
     9061 agccaacggt gcgcgagcaa aggtctcgaa aatcgccgaa ccgctcatta tatccgtccc
     9121 tggaaccgga aaatcaaaaa gccgcccacg cggcggcctt tgcggtttat ccggtttttc
     9181 ggcttccaag tcaacgaaaa cgtcgtgcgg caacgcttgg cggctagtgg gcagttggcg
     9241 ggcggcaacc catccaacag cgaccggtca agttggggaa aacccaaaaa accgattcgt
     9301 gttgcagttg ggactcttgt caccgagtcc gcccataagg tattcgtagt cgggaaataa
     9361 ctagatttcg tgcggcggat ctcttcaccc cggtatatgt ggtgttttgc ctggcggtgc
     9421 cgggacgaga agggattcct tgcgacgcac gatccaacag gagcgcagtc gcatgaactg
     9481 gactgacgaa cgcgtcgaac tcttgaagaa gctttggtcc gagggcctga gcgccagcca
     9541 gatcgctgcc cagctgggcg gcgtcagccg caatgcggtg atcggcaagg tacaccggct
     9601 gaagctttcg agccgtggcc gcgccaccgc cgcaccggcg cgccagaaga aaacggcaca
     9661 gccgggagcg gtcaagtcgg tttcccgcgc cgcgaccacc gttcgccaca tgacaagctc
     9721 gatcggggca accgcgctac agacgcagtt cgacgccgaa cctgcggccc gcatttatat
     9781 gcggccggcc accgagaatg tcgtcgtgcc gatttcgcgg cacctgcatc tggtcgaact
     9841 gaccgaacgc acctgcaaat ggccgaatgg cgacccgctt tccgaggact tccacttttg
     9901 cggcaacgac gccgccgaga ccggtcccta ctgcaagtat cacgcacgcc tggcgttcca
     9961 gccggcatcc gagcgtcgcc ggagcaggta aggggagcag ggtctgccgg gccgcgaggc
    10021 tgttgcaacg ccttgatcgc catcactatg cgctgttcgc cactcaccat ggcgtcagca
    10081 ttccctgccc tacagccagc cgttctttcg gaaccgccag tagaggaagc agcagatagc
    10141 cgcgatggcg gccagcacgg ctggatagcc atattccatc ttcagttccg gcatgtcggt
    10201 gaaattcatg ccgtaaatgc cggcaaaggc ggtcggcaca gccaatatgg cggcccatga
    10261 ggcgagtttc ttggaaatcg ccgtttcctg gctctgccct accagcaggc ttgcctcgaa
    10321 ggcgaaggcc agcacctcac gcaggctgtc gatcttttcc tgcacggtgc ggatatggtc
    10381 cgtcacgtcg cgaaacagcg ggtgcatggc ggcgtggatt tgcggcaggt ccgagctggt
    10441 cagccggcgg cagacctcca ccagcggaag agcggcattg
//
LOCUS       DASYCS010000358         9623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2133339,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000358 DASYCS010000000
VERSION     DASYCS010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 9623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9623
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2133339"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2018)
                     /locus_tag="VGM46_11190"
     CDS             complement(<1..2018)
                     /locus_tag="VGM46_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4193199.1"
                     /translation="MPLARTLSLAIRFSLREMRGGLSGFLIFLACIALGVAAIGGVNS
                     VARSISAGVADQGQGLLGGDMRFQLTQREAAMPELAFFKSLGTVSKSTNMRSMARLPD
                     GSDQALVEAKAVDTAYPLYGALETQPQLSHERLFGESDGAYGAAAPDLLFERLGLKPG
                     DKLKLGSATFQLRARLLTEPDALSDGFGFAPRLMVSTEGLAASGLVQPGSLVQQIYKL
                     RLPADTGDTQIKNIEKEAGEKFPQSGWSVRTRANAAPSLSSNIERFSQFLTLVGLTAL
                     VVGGVGVANAVRAYLESKRGIIATFKSLGASGRFVFTVYLVQILMIAGMGIAIGLVLA
                     ALMPFAASAALRSVIPVPAQGGFYPGALALAALFGVLTTLAFALLPLGRARDVPATAL
                     FREMGLQAGGMPRPVYVAMTLAIALVLAALAVLSSTDRHIAAIFVGATLASFLVLRGV
                     GILVQWLARRSPRSRFVALRLALGNIHRPGALTPSVVLSLGLGLTLLVTLALIDGNLR
                     RQIGGNLPEQAPNFFFVDIQAADLDAFSALIGKEAPAGTLAKVPMLRGRVMALNGVDV
                     AKVKVPAEGGWVLKGDRGLTYDAKKPENTTLSEGEWWPADYAGEPLVSFSAEEAHQIG
                     LKLGDTVTVNVLGRNVTAKIANFRQVEWETMGINFVMVFSPNTFAGAP"
     gene            complement(2018..2716)
                     /locus_tag="VGM46_11195"
     CDS             complement(2018..2716)
                     /locus_tag="VGM46_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4193200.1"
                     /translation="MAEAVIELKDVALTLGDGPSRVHVLNNVSLDVRSGEIIGIVGPS
                     GSGKSTLLMVLAGLERVDAGTVRIAGETLNGKSEDTIAAFRGRNIGIVFQSFHLIPNM
                     TALENVAVPLELTGHADPLGVAARELAAVGLSERVTHYPGELSGGEQQRVAIARALAP
                     GPRILIADEPTGNLDQATGRQVADLLFAKAAERAMTLVLVTHDPSLAARCSRQVAMRS
                     GRIEDAPEPARATA"
     gene            2779..3423
                     /locus_tag="VGM46_11200"
     CDS             2779..3423
                     /locus_tag="VGM46_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEY4193201.1"
                     /translation="MAVKHLTALFFSLFLGLVAAALPALAEPLKIVGFGDSLMAGYRL
                     GPGEGFTDRLQAALRAKGHEVTVENAGVSGDTTSGGLSRLDWSVPDGTQLVILELGAN
                     DMLRGVTPELAEKNLDEMLSRLKARKIPVLLAGMRAAPNLGQDYGKAFDAIYPKLAEK
                     YGVALYPFFLDGVAGNAALELDDGMHPNADGIGQMVDAILPTVEKAIAALPNPS"
     gene            3559..4152
                     /gene="thpR"
                     /locus_tag="VGM46_11205"
     CDS             3559..4152
                     /gene="thpR"
                     /locus_tag="VGM46_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEY4193202.1"
                     /translation="MPRLFTALEIPRDAALSLSLLRGGLPGARWIDVENYHLTLRFIG
                     DVEGHVADEIANALDRVRRPSFTMALSGVGAFGQKKPHAIWAGATASPDLTALQGEID
                     RICHRLGIPADPRKFTPHVTLARLRNSSPIEVAHYLSARGNFSTVPFRVGRFVLMSSR
                     DSVGGGPYVIEEAWPLWGKETRRQPEEAAAEASRLMR"
     gene            4228..4524
                     /locus_tag="VGM46_11210"
     CDS             4228..4524
                     /locus_tag="VGM46_11210"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006326971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEY4193203.1"
                     /translation="MAREKLDKVTAAKALADIDGWALADDGASIGRTFIFKNFSEAFG
                     FMTRCALSAEKMDHHPDWSNAYKTVKVKLSTDDAGGVTALDLELAKKMNRYFGG"
     gene            4607..4969
                     /locus_tag="VGM46_11215"
     CDS             4607..4969
                     /locus_tag="VGM46_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015314943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HEY4193204.1"
                     /translation="MVEKSNFGFSGFGGKPGNESEVRAKFWRTARKAARQIPFMEEVV
                     AAYYCALDKKTPLRAKAILLAALGYFVLPADAIPDIIFGLGFTDDIAVLTAAITAVRA
                     HITPAHRLAAKEAIADET"
     gene            5238..5744
                     /locus_tag="VGM46_11220"
     CDS             5238..5744
                     /locus_tag="VGM46_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HEY4193205.1"
                     /translation="MRGLIALVSGLAMFAAAASPALAQQATKIGQHNAWGTYSYQASG
                     GKVCYVLTVPTDKQPPTLDHGDMFFFVSQRPGQQVSYEPQFIAGYEFQKGSKATVTID
                     KKSFSMFTRGKSAWVENAAEEPVLIAAMKGGTDMKVEAKSGRGNTTTYVFSLKGISAA
                     LSSIASCK"
     gene            complement(5810..7168)
                     /locus_tag="VGM46_11225"
     CDS             complement(5810..7168)
                     /locus_tag="VGM46_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEY4193206.1"
                     /translation="MAGTSRRYPVLRRSRAMWGSRRVWQPRLVFWTGAVMVGLVSVLF
                     AKLADIAQSGFQAIAGDAGSWRGFLPLFITPLGFVVCAWAAHVYFPNSGGSGIPQAIA
                     ARHLRDAEERSALLSLKMIVGKIGLTLLGLLCGASISREGPTVQVGASIMMQVARWGG
                     MAQARGLILAGSAAGVAAAFNTPLAGIVFAIEEMGRTYQARTNGLVLTAVIFAGLASL
                     GLVGNYTYFGVAKGTVSFTSDAPLIVACGVIGGALGALFSLLTLKATRRIRRWNTLQP
                     LKRTLVIAGICGLVTAIIGISSGGMTFGTGYAQARGAIEGTPLPMFFFAGKLAAGLMA
                     TISGIPGGLFAPSLSVGAGIGSTLGLIFGGAAGTAALLGMAGYFAGVVQAPMTAFVII
                     LEMTGNHDNVIALMCASMLGYGTARMISHEPLYHALSRVFIADAIRRRRAEAPPAPAQ
                     QG"
     gene            7349..8584
                     /gene="rlmN"
                     /locus_tag="VGM46_11230"
     CDS             7349..8584
                     /gene="rlmN"
                     /locus_tag="VGM46_11230"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEY4193207.1"
                     /translation="MTLSLDLTTDAARDTLRLRALGAEKTSLVGLTRAEMAEALVASG
                     VVTERQAKMRASQLWHWLYVRGVSDFAHMFNISKELRAELDKHFTVARPEIVEEQIST
                     DGTRKWLFRFPPRGAGRPVEIETVYIPEEGRGTLCISSQVGCTLTCSFCHTGTQKLVR
                     NLTAEEILAQLLTARDKLGDFPDRDTPAGAIVPAEGRKVSNIVMMGMGEPLYNFEAVK
                     KALLIASDGDGLALSKRRITLSTSGVAPEITRTGEEIGVMLAISLHAANDDLRDLLVP
                     INKKYPLADLIAACRAYPGLSNARRITFEYVMLRDVNDSIDDAKALIKLLKGIPAKIN
                     LIPFNPWPGTNYQCSEWETIEKFADYINNAGYASPIRTPRGRDILAACGQLKSESERM
                     RKVDRLALEAMMIAGHGEA"
     gene            8581..9042
                     /locus_tag="VGM46_11235"
     CDS             8581..9042
                     /locus_tag="VGM46_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193208.1"
                     /translation="MSRRSLYLNRFAVILSGYLAAVIAAVVVTVFFLFLPTIHPNHAD
                     WGSFYRQLGGLPAGLQTGLVVTFPFALPGFLVALALAITCRWRKWLVYSVAGSVNALA
                     SLLIFNGLMGSSIVEMQDLLAASLSGGFVGGLAFWAVCGRHIAQWRLESAS"
     gene            9039..9536
                     /locus_tag="VGM46_11240"
     CDS             9039..9536
                     /locus_tag="VGM46_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193209.1"
                     /translation="MNRVLTYILRFAVILIGYMVATLAASAFIQLLFLGSAGLRLGEA
                     WELVTGSLLFSVPFTALFVGYFAFLPAVVLIVAGEVLGQRNWLFYALAGAAVGLAVAV
                     LFWQSSMMADGMGQMNTDPAFSGPRFVGLMIGGGIVGGLAYWLCAGRSAGNWRVAAKP
                     TSPER"
     gene            complement(9517..>9623)
                     /locus_tag="VGM46_11245"
     CDS             complement(9517..>9623)
                     /locus_tag="VGM46_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEY4193210.1"
                     /translation="AAMKASPKATRVVDYLLAGVFSAFALKILTAQAR"
BASE COUNT         1765 a         2984 c         3135 g         1739 t
ORIGIN      
        1 tggggcgcgc cggcgaaggt gttgggcgaa aagaccatga cgaaattgat gcccatggtt
       61 tcccattcga cctggcgaaa gttggcgatt ttcgccgtaa cattacggcc aagcacgttg
      121 acggtgacgg tgtcgccaag cttgaggccg atctggtgcg cctcctcggc ggaaaacgaa
      181 accagcggct cgccggcata gtcggccggc caccattcgc cttcgctcag cgtggtgttt
      241 tcgggcttct tcgcgtcgta ggtcaggccg cggtcgccct tcagcaccca gccgccttcg
      301 gccggcacct tcaccttggc gacatcgacg ccgttcagcg ccatgacgcg gccacgcagc
      361 atcggcacct tggccagcgt gccggccggg gcttccttgc cgatcagcgc cgaaaaggcg
      421 tcgaggtcgg cggcctgaat atccacgaag aagaagttgg gcgcctgttc gggcaaattg
      481 ccgccgattt ggcggcgcag attgccgtcg atcagcgcca gcgtgaccag cagcgtcagg
      541 ccaaggccga gcgaaagcac gacggagggc gtcagcgcgc cgggccggtg gatgttgccg
      601 agcgccaggc gaagcgcgac gaagcgcgag cgcgggcttc tgcgcgccag ccactgcacc
      661 aggattccga cgccgcgcag caccagaaag gaggccagcg tcgcgccgac gaagatcgcc
      721 gcgatatggc ggtcggtgga cgaaagcacc gccagcgccg ccagcacgag cgcgatggcg
      781 agcgtcatcg ctacatagac cgggcggggc atgccgccgg cttgcaggcc catttcacga
      841 aacaaggccg ttgccggcac atcgcgggcg cggccaagcg gcagcaaggc gaaggcgagc
      901 gtggtcagca cgccgaacag ggcggcaagg gcgagcgcgc cgggataaaa gccgccctgc
      961 gccggtacgg ggatgacgga gcggagcgcg gcgctggcgg cgaacggcat cagggccgcc
     1021 agcacgaggc cgatggcaat gcccatgccg gcgatcatca ggatctggac gagatagacg
     1081 gtgaagacga aacgccctga cgcgccgagg ctcttgaagg tggcaatgat gccgcgcttg
     1141 gattccagat aggcgcgcac cgcgttggcg acgccgacgc cgccgaccac caatgcggtg
     1201 aggccgacca gcgtgaggaa ttgcgagaag cgctcgatgt tggacgacag cgatggcgcg
     1261 gcattggcgc gggtgcgcac cgaccagccg gactgcggaa acttttcgcc ggcctctttc
     1321 tcgatgttct tgatctgagt gtcgccggta tcagcaggca ggcgaagctt gtagatttgc
     1381 tggacaaggc tgcctggttg cacaagcccg gaagcggcaa ggccttccgt ggaaaccatc
     1441 agtctcggcg caaagccgaa gccgtcggaa agcgcgtcgg gttcggtaag gagcctggcg
     1501 cgcagctgga atgtcgcgct gccgagtttc agcttgtcgc caggtttcag gcccaggcgc
     1561 tcgaacagga ggtccggcgc ggccgcgcca taggcgccat cgctctcacc gaatagtcgc
     1621 tcgtgggaaa gctgcggctg cgtttccaac gcgccataaa gcggataggc ggtgtccaca
     1681 gccttggcct cgaccagcgc ctggtcggaa ccgtccggca gccttgccat ggaacgcatg
     1741 ttggtgcttt tcgagaccgt gccgaggctt ttgaaaaagg cgagttccgg catggcggcc
     1801 tcgcgctggg tcagctggaa gcgcatgtcg ccaccaagca ggccctggcc ctgatcggcg
     1861 acgccggcgg aaatcgaacg ggcgaccgaa ttgacgccgc cgatggcggc gacgccaagc
     1921 gcgatgcagg cgagaaaaat caggaagccg gaaaggccgc cgcgcatttc gcgcagtgaa
     1981 aaacggatgg cgagagaaag cgtgcgcgcc agcggcatca agccgtcgcc cttgccggtt
     2041 ccggcgcgtc ctcgatccgg cccgagcgca tcgccacctg ccgggaacag cgggcggcga
     2101 gcgacgggtc gtgggtgacc agaaccagcg tcatggcgcg ctcggcggcc ttggcgaaca
     2161 acaaatccgc cacttgcctg ccggtcgcct ggtcgagatt gccggtcggc tcgtcggcga
     2221 tcaggatgcg cgggccgggc gccagcgcgc gggcaatcgc cacgcgctgc tgctcgccgc
     2281 cggaaagttc gccgggatag tgggtgacac gctcgctgag gccgaccgcg gccagttcgc
     2341 gcgcggcgac accgagcggg tcggcatgac cggtcagttc cagcggcacg gcgacatttt
     2401 ccagcgccgt catgttggga atgagatgaa acgactggaa gacgatgccg atatttcgcc
     2461 cgcgaaaggc ggcgatcgta tcctcgctct tgccgttcag cgtctctccg gcgatgcgca
     2521 ccgtgccggc atcgaccctt tccagcccgg ccagcaccat cagcaatgtc gattttcccg
     2581 atccggaggg gccgacgatg ccgattatct cgccgcttct cacatcgagg ctgacattgt
     2641 tcaggacatg cacgcgcgac gggccgtcgc ccagcgtcag cgccacgtct ttcagctcga
     2701 tgacggcttc tgccaaggat aaatcgtcct atatgggagg agaggcgaag gatgccttgg
     2761 tcatatgggg gctgccgaat ggctgtcaaa catctaaccg ccctgttttt ctcacttttt
     2821 cttgggcttg tcgcggcggc cctgcctgcg ctggccgaac cgctgaagat cgtcggcttc
     2881 ggcgacagcc tgatggccgg ctatcggctt gggccgggcg aagggtttac cgaccggctg
     2941 caagcggcgt tgcgggcaaa aggccatgag gtgacggtgg aaaatgccgg cgtttcgggc
     3001 gacaccacca gtggcgggct ttcgcggctg gattggtcgg tgccggacgg cacgcaactg
     3061 gtgatcctgg agcttggcgc caacgacatg ctgcgcggcg tgacgccgga actggcggaa
     3121 aagaacctcg acgaaatgct gtcgcgcctg aaggcgcgca aaatcccggt tctgctggcg
     3181 ggcatgcgcg cggcgcccaa tctcggccag gactatggca aggcgttcga cgccatctat
     3241 ccgaaactgg ccgaaaaata cggcgttgcg ctctatccgt tctttctgga cggcgtcgcc
     3301 ggcaatgccg cgctcgaact ggacgacggc atgcatccta acgcggatgg gatcggccag
     3361 atggtggatg ccatcctgcc aacggtggaa aaggcaattg ccgccctgcc caacccgtca
     3421 tgaaacgcaa ccaaatgacg ccggattgct ttccgtcacc aaggttacac atccggctgt
     3481 gaccaataaa gcgcttgctc cgtttggcta tcggtgattc gctggggcca gacttcgatt
     3541 cgatgaaggg aggctctcat gccgcgtctt ttcaccgccc tcgaaattcc gcgcgatgct
     3601 gcgctgtcgc tttccctgtt acggggaggc ttgcctggcg cccgctggat cgacgtcgag
     3661 aactatcacc tgaccctgcg cttcatcggc gatgtcgaag ggcatgtcgc cgacgagatc
     3721 gccaatgcgc tcgaccgggt gcggcggcct tccttcacca tggcgctgtc gggtgtcggc
     3781 gccttcggtc agaagaagcc gcacgccatc tgggccggtg cgacggcatc gccggacctc
     3841 accgccttgc agggcgaaat cgatcgcatc tgccatcggc tcggcattcc cgccgacccg
     3901 cgcaagttca ccccgcatgt gacgctggcg cggctgcgca attccagccc catcgaagtg
     3961 gcgcattatc tttcggcgcg gggaaatttc tcgacggtgc cgttccgggt cgggcgcttc
     4021 gtcttgatgt cgtcgcgcga ttcggtcggc ggcggtccct acgtgatcga ggaagcgtgg
     4081 ccgctatggg gcaaggagac acgccgccag cccgaagagg ccgccgccga agcctcgcgg
     4141 ctgatgcggt agcagcgtcg atacaggctg tgcgtgcgcc gttccgatgc tatgttgaag
     4201 gcaggaacga cggacaagga tgcttcgatg gcacgcgaga aactggacaa ggtcaccgcc
     4261 gccaaggcgt tggccgatat tgacggctgg gcgctggccg atgacggagc ctcgatcggc
     4321 cgcaccttca tcttcaagaa tttttccgag gctttcggct tcatgactcg ttgtgcgctg
     4381 tcggcggaaa agatggatca ccatcccgac tggtcgaatg cctacaagac cgtgaaggtg
     4441 aagctgagca cggacgatgc cggcggcgtg acggcgctcg accttgaact ggccaagaaa
     4501 atgaatcgtt atttcggcgg ctgatattgc aggcatcttg cagcgggccg gcggcttcac
     4561 cacatttgcg gcaagtggcg gtagccgttg cggttggaga aacagagtgg tggaaaaatc
     4621 gaacttcggg ttttcaggct tcggcggcaa gccgggcaat gaaagcgaag tgcgagccaa
     4681 attctggcgc acggccagga aagcggcgcg ccagatccct ttcatggaag aggtggtcgc
     4741 cgcctattat tgcgcgctgg acaaaaagac gcccctgcgc gccaaggcca tcctgctggc
     4801 cgcgctcggc tatttcgtac tgccggcgga tgccattccc gatattatct tcgggctggg
     4861 gttcaccgat gacattgccg ttttgaccgc ggcaatcacc gccgttcgcg cccatattac
     4921 gccggcgcac cggctggccg cgaaagaggc catcgccgac gaaacttaag gcggccttta
     4981 ccttcaaccg gcgcgccatc ccgcgcttgc gcaaaaagac aaactcttgc cgctttcctg
     5041 cctttcaagt cgcagcaggg acattgctct ggttgccagc aaggcagccg gcgcgcaagt
     5101 ggcggtggtt tgtcgatggc gaaagttctt ttctctcaga acttcatcct ttggtaaccc
     5161 agattaaatc aaaataaagc gagtggcagg acgccaagcc ggcctgcttc cgcataattt
     5221 ggaatacggg aaaaacgatg cgcggattga tagcactagt ttcgggcctt gcgatgtttg
     5281 cggcggcggc ttcgccggcg ctggcgcagc aggcgaccaa gatcggccag cataatgcct
     5341 ggggcaccta cagctaccag gcttcgggcg gcaaggtctg ctacgtgctg acggtgccga
     5401 ccgacaagca gccgccgacg ctcgaccatg gcgacatgtt ctttttcgtc agccagcgtc
     5461 ccggccagca ggtctcctat gagccgcagt tcatcgccgg ctatgaattc cagaaggggt
     5521 cgaaggcaac cgtcaccatc gacaagaaat ccttctccat gttcacgcgc ggcaagtcgg
     5581 cctgggtgga aaacgccgcc gaggagccgg tgctgatcgc cgccatgaag ggcggcaccg
     5641 acatgaaggt cgaggccaag tccgggcgcg gcaacaccac gacatatgtg ttttcgctga
     5701 agggtatctc cgccgcgctc agctccatcg ccagctgcaa gtagcggctt tagggtttta
     5761 ggcgcgaagg ccgggcaatc gacccggcct ttttgctttg aacccgaact cagccctgtt
     5821 gggctggcgc gggcggcgct tcggcgcgcc ggcgacggat ggcatcggcg atgaagacac
     5881 gcgacaatgc gtggtaaagc ggctcatgcg agatcatgcg cgcggtgccg tagccgagca
     5941 tcgaagcaca catcagtgcg atgacattat cgtgattgcc ggtcatttcc agaatgatga
     6001 cgaaagccgt catcggcgcc tgcaccacgc cggcgaaata gccggccatg ccgagcagcg
     6061 ctgccgtgcc ggcggcgccg ccgaagataa ggccgagggt ggagccgatg ccggcgccca
     6121 ccgagagcga tggcgcgaac aggccgccgg gaatgccgga gatggttgcc atcaacccgg
     6181 ccgccagctt gcccgcgaag aagaacatcg gcaacggcgt gccttcaatg gcgccgcgcg
     6241 cctgggcata gccggtgccg aaggtcattc ccccggacga gatgccgata attgccgtga
     6301 ccagcccgca aatgccggca atgacgagcg ttcgcttcag cggttgcagg gtgttccacc
     6361 gccttatgcg ccgcgtcgcc ttcagcgtca gcagggagaa caaggctccc agcgcgccgc
     6421 cgatgacgcc gcaggcgacg atcaaaggcg catccgatgt gaatgatacg gtgcctttgg
     6481 caacgccgaa ataggtatag tttccgacca ggccgagaga ggcgaggccg gcgaagatca
     6541 cggccgtcaa gaccaaccca ttggtgcgcg cctgataggt ccgccccatt tcctcgatgg
     6601 cgaagacgat gcctgcgagc ggcgtgttga aggcggcggc gacgccggcc gccgacccgg
     6661 ccaggatcaa cccgcgcgcc tgcgccatgc cgccccaacg cgccacctgc atcatgatcg
     6721 atgcgcccac ctgcaccgtc gggccttcgc ggctgatcga ggcgccgcac agcagaccga
     6781 gaagggttag gccgatcttg ccgacgatca tcttcaggga cagcaaggcg ctgcgttctt
     6841 cggcatcgcg caggtgccgc gcggcaatgg cctgcggaat gccgctgccc cccgaattgg
     6901 gaaaatagac gtgggcggcc catgcgcaca ccacgaaacc aagcggggtg atgaaaagtg
     6961 gcaggaaacc acgccagctg cccgcatcgc cggcgatggc ctggaagcct gactgggcga
     7021 tgtcggccag ttttgcaaac aggacgctga ccaggccgac catcaccgcg ccggtccaga
     7081 atacgaggcg cggctgccag acccgccgcg agccccacat ggcgcgagag cggcgcaaaa
     7141 cgggatatct tcgcgaagta ccggccatct gtcgtcctgt cgttgatgct gccgaagccg
     7201 tagcacggca atgcgcccgg atgaaccggc atgtccggcg cgcaaaccgt tgcgggcatg
     7261 actcttgcga caatccatgc tatgcgccgc gcttgatttc ctgaaccctg ctggatcggc
     7321 ccttcgccat ccccatataa gcgtcgccat gaccctttcg ctcgacctga ccaccgatgc
     7381 cgcgcgcgat acgctgcgcc tccgcgcctt gggcgctgaa aagacgtcgc ttgtcgggct
     7441 gacgcgggct gaaatggccg aggcgctggt ggcgtccggc gtcgttacag agcggcaggc
     7501 caagatgcgc gccagccagc tttggcactg gctctatgtg cgcggcgttt ccgatttcgc
     7561 gcatatgttc aacatctcca aggaactgcg cgccgaactg gacaagcact tcaccgtggc
     7621 gcggcccgag atcgtggaag aacaaatctc aaccgacggc acccgcaagt ggctgtttcg
     7681 ctttccgccg cgcggggcgg gccgtccggt cgagatcgag acggtctata ttccagagga
     7741 agggcgcggc acgctgtgca tctccagcca ggtaggctgc acgctgacct gctcgttttg
     7801 ccataccggc acgcagaagc tggtgcgcaa cctgacggcg gaagaaatcc tggcgcagct
     7861 gttgaccgcg cgcgacaagc ttggcgattt tccggaccgc gatacgccgg ccggcgcgat
     7921 cgtgccggcg gaagggcgca aggtttccaa catcgtcatg atgggcatgg gcgagccgct
     7981 ctacaatttc gaggcggtca agaaggcgct gctgattgct tccgatggcg acgggctggc
     8041 gctgtcgaag cgccgcatca cgctgtccac ctccggcgtt gcgccggaaa ttacccgcac
     8101 cggcgaagag atcggcgtta tgctggccat ctcgctgcat gccgccaatg acgatctgcg
     8161 cgaccttctg gtgccgatca acaagaaata tccgcttgcc gatctgatcg ccgcctgccg
     8221 cgcctatccg ggcctttcca atgcgcgccg catcactttc gaatatgtga tgctgcgcga
     8281 cgtcaacgat tccatcgacg acgccaaggc gctgatcaaa ctcttgaaag gcattcccgc
     8341 caagatcaac ctgatcccgt tcaatccgtg gcccggcacc aactaccagt gctcggagtg
     8401 ggaaacgatc gagaagttcg ccgattatat caacaatgcc ggctatgcct cgccgatccg
     8461 cacgccgcgc ggccgcgata ttctcgccgc ctgcggccag ctcaagtcgg agtccgagcg
     8521 catgcgcaag gtcgaccggc tggcgctgga ggcgatgatg attgcggggc acggcgaggc
     8581 gtgagccgcc ggtcgcttta cctcaaccgc ttcgccgtta ttctttcagg ctatttggcc
     8641 gcggtcattg ccgctgtcgt ggtgacggtt ttcttcctgt ttctgccgac catacacccc
     8701 aatcatgccg attggggttc gttttatcga cagctgggtg gtcttcccgc cggattgcag
     8761 accggcctgg tcgtgacgtt cccgttcgcg ttgcccggat ttctagtcgc attggcgctg
     8821 gcaatcacct gtcgatggcg caaatggctg gtctattcgg ttgcaggcag cgtcaatgcg
     8881 ctggcgtcgc ttttgatctt caatggtttg atgggttcgt cgatcgttga aatgcaggat
     8941 ctgcttgccg ccagtctttc cggcggtttc gtgggagggc ttgcgttctg ggcggtttgc
     9001 ggtcgtcaca tagcccaatg gagactggaa agcgcatcgt gaaccgcgtt ctcacctata
     9061 tacttcgctt cgccgtcatc ctgatcggct acatggtggc gacgctggcc gccagcgcct
     9121 tcatccagct gcttttcctt ggctcggccg ggttgcggct tggtgaggcg tgggaactcg
     9181 ttacgggttc gttgctgttt tcggtcccgt tcaccgcgct tttcgtcggc tatttcgctt
     9241 tcctgccggc cgtggtgctg atcgtggcgg gcgaagtgct tggccagcgc aactggctgt
     9301 tctacgcgct ggcgggggcc gccgtcgggc tggcggtggc cgtcctgttc tggcaatcgt
     9361 cgatgatggc cgacggcatg ggccagatga acaccgaccc ggccttcagc ggcccgcgtt
     9421 tcgtcgggct gatgatcggc ggcggcatcg tcggcggcct tgcctactgg ctatgcgccg
     9481 gccgttcggc gggcaactgg cgtgtggccg cgaagcctac ctcgcctgag cggtgaggat
     9541 cttgagggcg aaggccgaga atacgccggc aagcaggtag tcgaccacgc gggtcgcctt
     9601 cgggctggcc ttcatcgcgg ccg
//
LOCUS       DASYCS010000359         6289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3145641,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000359 DASYCS010000000
VERSION     DASYCS010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 6289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6289
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3145641"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..286
                     /locus_tag="VGM46_11250"
                     /pseudo
     CDS             75..286
                     /locus_tag="VGM46_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612405.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            429..1793
                     /locus_tag="VGM46_11255"
     CDS             429..1793
                     /locus_tag="VGM46_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015319325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HEY4193211.1"
                     /translation="MPVELVATKSKNSAPIHLIAKNGLADAGLDKSELAWARANGFSG
                     EAGKTLVLPGTGGSVAGALFGTGKGEGALALGALAKALPEGDWHFASSPDDPGLAALS
                     LVLGGYIFARYGKAPGKALRLAVPEGADARHLQRLAAGVFLARDLVNTPTNEMGPEAL
                     EGAVRTLAKAHKAEVSVISGDDLLAKNFPMIHAVGRASVEAPRLVELTWGPKNAPKVT
                     LVGKGVCFDTGGLDIKPASGMLLMKKDMGGAANVLGLASMVMAAKLKVRLRVLIPAVE
                     NSIAGNAFRPGDVLTSRKGITVEIGNTDAEGRLVLADALALADDETPQMLVDMATLTG
                     AARVALGPDLPPFYTHDDALAAEIAVASNAVEDPLWRMPLWQPYQARLASKIADVNNV
                     TTDGYAGSITAALFLQRFVEKTPAWVHFDIFAWNPWDRPHGPVGGEAQGIRALEKVIS
                     SRFV"
     gene            1854..2204
                     /locus_tag="VGM46_11260"
     CDS             1854..2204
                     /locus_tag="VGM46_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010657792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEY4193212.1"
                     /translation="MAISMRPIQALRLWQQVMLSQVRDDAPDLTMRQTAILLTIYLDA
                     PPHTVRGLAARLDVTKPVITRALDTMGALKLVSRHRDENDKRNVLIKRTVEGALYVER
                     FGDDIIAKALELPI"
     gene            2214..3074
                     /locus_tag="VGM46_11265"
     CDS             2214..3074
                     /locus_tag="VGM46_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEY4193213.1"
                     /translation="MTVRDARLHAFRADLADARLQGEVQADRFVTARPARVTASVADL
                     RKAPALDAGVNTQFLFGDDVLVFEEKQGWAWAQGVRDGYVGYIATSELGARAEAPTHV
                     VSVARTFVYPGPDLRFPLSGQLSMGSTVTVAGDAETRGTHYALLPSGEAVIAAHLRPI
                     ADHAADYVAVAEQFLGTPYLWGGASGFGIDCSGLVQLSMRMAGRNVLRDSDMQAADLG
                     EPVEPGPDFSGLRRGDLVFWKGHVAIMTDAKNMIHANGHTMLVSREGLKEAVGRIGYL
                     YGGPTGFRRP"
     gene            complement(3096..3761)
                     /locus_tag="VGM46_11270"
     CDS             complement(3096..3761)
                     /locus_tag="VGM46_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013527995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEY4193214.1"
                     /translation="MTLKINGADIIDVKSFIDENLPLAAVPSLPEIRLHLAHPGSRLK
                     QLEQKGNAAAPYWAYPWPGGVALARYVFDHPETVRGRRVMDLGSGSGLVAIAAAKAGA
                     SQVVAAEIDRNAIAALELNLAANGVIAAIIDRDITGDEPAAIDLILVGDLFYERRLAA
                     RVTAFLDRCTGEGIEVLVGDIGRKHLPLPRLRPLAEYGVADFGDAKGEAPRRGHVFSF
                     EAG"
     gene            complement(3758..4300)
                     /gene="moaB"
                     /locus_tag="VGM46_11275"
     CDS             complement(3758..4300)
                     /gene="moaB"
                     /locus_tag="VGM46_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006726641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HEY4193215.1"
                     /translation="MDRIDESRPFIPLNIAVLTVSDTRAMADDKSGQTLADRITQAGH
                     ILADRAIVTDDAEKIRETVLAWSRDEKVDVVITTGGTGFTGRDVTPEALETIFEKRMD
                     GFSAVFHRISYDKIGTSTVQSRATGGVVNATFVFVLPGSPGACKDAWDGILKPQLDYR
                     HMPCNFVEIMPRLDEHLKRS"
     gene            complement(4287..5192)
                     /locus_tag="VGM46_11280"
     CDS             complement(4287..5192)
                     /locus_tag="VGM46_11280"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006201978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEY4193216.1"
                     /translation="MQSVTAAAGASLAPAKINLALHVTGQRADGYHIIESLAVFTRFG
                     DRIEAEPAEQDTFGVSGKYATAVPDDSGNLVLKARDALRQRFGADCAPPVAISLEKNL
                     PVASGIGGGSSDAAATLRVLAKLWNLAVDEAELARIGLTLGADVPMCLAAQPLIARGI
                     GEELETLPDFPALGLVLVNPNVAVSTAEVFAALERRDNGKLPPLPPTLDFHSLRNWLE
                     ITRNDLQPAAQKLHPAIGRALSALKRADAGFARMSGSGATCFGLFETGNVAKRAAASI
                     RSHYPEMFVAATRSMAAGADAHGQN"
     gene            complement(5198..5383)
                     /locus_tag="VGM46_11285"
     CDS             complement(5198..5383)
                     /locus_tag="VGM46_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006201979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193217.1"
                     /translation="MPQIIFFAAVGVAAYVGYRTFVREAERVTAKLRRAEKQHANGTM
                     GTLVKDPVTGEYRLAKD"
     gene            complement(5536..>6289)
                     /locus_tag="VGM46_11290"
     CDS             complement(5536..>6289)
                     /locus_tag="VGM46_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEY4193218.1"
                     /translation="PSLSLASTAGLIEKAFAFDAPAVAISINSPGGSPVQSRLIYQRI
                     RDLAVEKNKRVLVFTEDVAASGGYMIAIAGDEIIADPSSIVGSIGVVSASFGFPELLK
                     KIGVERRVYTAGQNKATLDPFKPEKKEDIDRLKALQLEVHETFIDMVKARRGARLKDD
                     PDLFTGLFWTGKKGLELGLVDGLGDMRTTLKAHFGPKTRMKLVSMPRGLFGKIGLFGS
                     SGGFSAPSVAEAAAAGLIDAAQERALWGRFGL"
BASE COUNT         1123 a         1958 c         2037 g         1171 t
ORIGIN      
        1 tcgagtagcc gaggcacgat gacggccggc caattccaac ggtaaaccga ggccggattt
       61 gataacttca gcgtatgctg aacgctgccg atcttcctga taacattgcc gtcctgaagg
      121 cgatgatgat cgcacgtgaa ggccgcaagg atgagcggat cgaaaggcgg aaaaggctgg
      181 ttaacgcttt caagcaggcg gcgttcggcg cagatccgag aagaccgatc ctgatcagtt
      241 tgatctggca ctggaggatc tggaaacggc catcgcgacc gtccaaatgc cgggaccaaa
      301 cagctggctg ggtgctagta cgtcacgtct caaagattta caccgccttg ctttgctccg
      361 gctcaataac cacaataacg cttcgttaag aggccgaccc tcgagtcgcg tgcctcaggg
      421 agtgttggat gccggttgaa cttgtcgcca cgaaatcgaa gaactcggcc cccattcacc
      481 tgattgcgaa aaatggcctg gcggacgcgg gattggataa atccgaactt gcctgggcgc
      541 gggccaacgg cttttccggc gaggcgggca aaacgctggt gctgcccggc actggtggct
      601 ctgtcgccgg cgcgttgttc ggcaccggca agggggaggg cgcactggcg cttggcgcgt
      661 tggctaaggc gctgccggag ggtgactggc acttcgcgtc atctccggac gaccccggcc
      721 ttgcggcgct gtcgctggtg ctgggcggct atattttcgc ccgttacggc aaggcgcccg
      781 gcaaggcgtt gcggctggcc gtgccggaag gggcagacgc gcgccatctc cagcgccttg
      841 ccgccggcgt tttccttgcc cgtgacctgg tcaatacgcc gaccaacgag atgggaccgg
      901 aagcgctgga aggcgcggtc cggaccttgg cgaaggccca taaggccgag gtttccgtca
      961 tcagcggtga cgatcttctg gcgaaaaact ttccgatgat ccacgcggtc ggccgggctt
     1021 cggtcgaagc gccgcgcctg gtcgagctga catgggggcc gaagaacgca ccgaaggtga
     1081 cgctggtcgg caagggcgtc tgcttcgata ccggcggtct cgacatcaag ccagcaagcg
     1141 gaatgctgtt gatgaagaaa gacatgggcg gcgcggccaa cgtgcttggc ctcgcttcca
     1201 tggtcatggc cgccaagctg aaagtcaggc tgcgcgtcct cattcccgcc gtggaaaact
     1261 cgattgccgg caacgccttc cgcccgggcg acgttttgac cagccgcaag ggcatcaccg
     1321 tcgagatcgg caataccgac gctgaagggc ggctggtgct ggctgatgcg ctggcgctgg
     1381 ccgacgacga gaccccgcag atgctggtcg atatggcgac cttgacgggc gcggcgcgcg
     1441 tagcgcttgg ccccgacctg ccgcctttct acacccatga cgacgccctc gccgccgaga
     1501 tcgctgtcgc ctcgaacgcc gtcgaggatc cgctgtggcg catgcctttg tggcagccct
     1561 atcaggcgcg gctggcgtcg aagatcgccg acgtcaacaa cgtcaccacc gacggctatg
     1621 ccggctcgat cacggcggct cttttcctgc aacgcttcgt ggaaaagacg cccgcctggg
     1681 tgcatttcga cattttcgca tggaacccgt gggaccgccc gcatgggccg gtcggcggcg
     1741 aggcgcaggg catccgtgcg ctggagaagg tgatttccag ccgcttcgtt taggccgcga
     1801 cattcgatcc tgactgaaac ggaagcgaaa cgaattgccg gcatattatt tcgatggcga
     1861 tttcgatgcg tccgattcag gctttgcgac tgtggcagca agtaatgctg tcacaggtgc
     1921 gcgacgatgc gccggacctc accatgcgcc aaacggcgat cctgctcacc atttacctcg
     1981 atgcgccgcc gcataccgtg cgcgggctgg cggcaaggct cgatgtcacc aagccggtca
     2041 tcacccgcgc gctcgatacg atgggcgcgc tgaaactggt gtcgcgccac cgcgacgaga
     2101 acgacaagcg caacgtgctc atcaagcgca cggtggaagg cgctctctat gtcgaacgct
     2161 ttggcgatga tatcattgcc aaagcccttg aattgccgat ttgaaagctg ccgatgaccg
     2221 tccgcgacgc ccgcctccat gctttccgcg ccgatctggc cgatgcgcgc ctgcaaggcg
     2281 aagtgcaggc cgaccgtttt gtcacggcca ggccggcgcg ggttacggct tccgtcgcgg
     2341 atttgcgaaa agcgccggcg ctcgatgccg gcgtcaacac gcagttcctg tttggcgatg
     2401 acgtgctggt tttcgaggag aaacaaggct gggcctgggc gcagggcgtg cgcgacggct
     2461 atgtcggcta tatcgcgacc tctgaactgg gcgcgcgcgc cgaggctcca acgcatgtcg
     2521 tgtcggtggc gcgcaccttc gtctatccgg ggccggatct gcgtttcccg ttgagtggcc
     2581 aactatcgat gggttcgacg gtcaccgtcg caggcgacgc cgagacgcgc ggaacgcact
     2641 atgcgctgct gccttccggc gaggcggtga ttgccgcgca tttgcggcct attgccgacc
     2701 atgccgccga ctatgtggct gttgccgagc agtttctggg cacgccctat ctttggggtg
     2761 gcgcgtcggg cttcggcatc gattgctccg ggcttgtgca gctttccatg cggatggcgg
     2821 gcaggaacgt cctgcgcgat tccgacatgc aggcggcaga cctgggcgaa ccggtggaac
     2881 cgggaccgga tttctccggg ctgcggcgcg gcgatctggt gttctggaag ggccatgtcg
     2941 cgatcatgac cgacgcgaaa aacatgatcc acgccaacgg ccacaccatg ctggtgtcgc
     3001 gcgaagggct gaaggaagcg gtcggccgca tcggctatct ttatggcggc ccgaccggtt
     3061 ttcgccggcc ctaagcccga cctggttcaa aaggttcaac cggcttcaaa agagaaaaca
     3121 tggccgcgcc tgggcgcttc gcccttggca tcgccgaagt ccgccacgcc atattccgca
     3181 agcggtcgca ggcggggcag gggcaggtgt ttcctgccga tgtcgccaac cagcacctcg
     3241 atgccttcgc cggtgcatcg gtcgagaaat gcggtgacgc gggcagcgag gcggcgctcg
     3301 tagaacaggt cgccaacgag gataagatcg atcgccgccg gctcgtctcc ggtgatgtcg
     3361 cggtcgatga tggcggcgat gacgccattg gcggcgaggt tcagttccag ggcggcaatc
     3421 gcgttgcggt cgatctcggc ggccaccacc tggcttgcgc ccgccttggc tgcggcaatg
     3481 gcaacgaggc cggaaccgga gccaaggtcc attacgcgcc ggccccgtac ggtttcagga
     3541 tggtcgaaaa catagcgcgc aagcgcgacg ccgcccggcc atggataggc ccaatagggt
     3601 gcggcggcgt tgcctttctg ttcgagctgc ttcaaacggc tgccgggatg cgccagatgc
     3661 agccggattt ccggtaaaga cggcaccgct gccagcggca ggttctcgtc gatgaaggac
     3721 ttcacgtcga ttatatctgc gccgtttatt tttaacgtca cgagcgtttc agatgctcgt
     3781 ccagccgggg catgatctcg acgaaattgc agggcatgtg ccgatagtcg agctgcggct
     3841 tcaagatgcc gtcccaggca tccttgcagg cgccgggcga tcccggcagc acgaacacga
     3901 aggtcgcatt gaccacgccg ccggtagctc ggctctgcac ggtcgacgtg ccgatcttgt
     3961 cgtaggaaat acgatggaaa accgccgaaa agccgtccat gcgcttctcg aagatcgttt
     4021 ccagcgcctc tggcgtcacg tcgcggccgg tgaagccggt gccgccggtg gtgatgacga
     4081 catcgacctt ttcatcgcgc gaccaggcca gcaccgtttc gcggattttt tcggcatcgt
     4141 cggtaacgat ggcgcggtcg gcaaggatat ggcccgcctg tgtgatgcgg tcggccagcg
     4201 tctggcccga tttgtcgtcg gccatggcgc gcgtatccga cacggtcagc accgcgatgt
     4261 ttaacgggat gaacgggcgg ctctcatcaa ttctgtccat gcgcgtcggc ccccgctgcc
     4321 atgctgcgtg tcgcggcgac gaacatttcc ggatagtggc tgcgaatgga agccgccgcg
     4381 cgcttggcga catttccggt ttcgaacagg ccaaagcagg tcgcgcccga accggacatg
     4441 cgggcaaagc cggcatcggc ccgtttcagc gccgacagcg ccctgccgat ggcgggatga
     4501 agcttttgcg cggcaggttg cagatcgttg cgggtgattt ccagccagtt gcgcaggctg
     4561 tgaaaatcga gggtcggcgg cagcggcggc agcttgccat tatcgcgccg ctccagcgcg
     4621 gcaaagacct cggctgtcga cacggccaca ttcggattga ccagaaccag gccgagcgcg
     4681 ggaaaatcag gcagggtttc cagttcctcg ccgatgccgc gtgcgatcag cggctgggcc
     4741 gccaggcaca tcggcacgtc cgcgccgagc gtcagcccga tgcgcgccag ttcggcctcg
     4801 tccacggcaa ggttccagag cttcgcaagc acgcgcagcg tcgccgccgc atcgctcgat
     4861 ccgccgccga tccctgaggc gaccggcagg ttcttttcaa gcgagatggc aacgggtggc
     4921 gcgcagtccg cgccaaatcg ctgccgcaac gcatcgcgcg ccttcagcac cagattgccg
     4981 ctatcgtcgg gtacggcggt ggcatatttg ccggaaacgc cgaacgtgtc ctgctcagcc
     5041 ggctccgcct cgatgcggtc gccgaagcgg gtgaacaccg caaggctctc gatgatgtgg
     5101 tagccgtcgg cccgctgccc ggttacgtgc agcgcaaggt tgatcttggc tggcgcaagg
     5161 cttgcacccg ccgcggcagt tacggattga atgatgttca atccttggcc agccggtatt
     5221 cgccggtaac ggggtccttg accagcgttc ccatcgtgcc gttggcgtgc tgcttttcag
     5281 cccggcgcaa cttggcggtc acacgctcgg cttccctgac gaaagtgcgg tagccgacat
     5341 aggcggcgac accgacggcg gcgaagaaaa tgatctgcgg catgtcaaaa ctctcctttg
     5401 ctttcgcgcg ccggaggcct ctatgggtcg atcgaggctc catgctagcg cataagtcga
     5461 gaaatcgaaa ttgctttctg ggcaaaccca tgcggcaaaa aatcggcttg cctcaaaccc
     5521 gcggcttgcg gccgcttaaa gaccgaaacg tccccacagc gcgcgctcct gcgccgcgtc
     5581 gatcaggccg gccgccgccg cttcggccac actcggcgct gaaaagccgc cgctggagcc
     5641 gaacaggccg atcttgccga acagcccgcg cggcatggaa accagcttca tgcgcgtctt
     5701 cggcccgaaa tgcgccttca aggtcgtccg catgtcgccc agcccatcga ccaggccaag
     5761 ttcgaggccc ttcttgccgg tccagaacag cccggtgaac aggtccggat cgtctttcag
     5821 cctggcgccg cgccgcgcct tcaccatgtc gatgaaggtt tcatgcacct ccagttgcag
     5881 cgctttcagc cggtcgatgt cttctttctt ttccggcttg aaggggtcga gcgtcgcttt
     5941 gttctggcct gctgtataga cccggcgctc gacgccgatt ttcttcaaca gctccggaaa
     6001 gccgaacgag gccgagacga cgccgatcga gccgacgatg gaggaggggt cggcgatgat
     6061 ctcgtcgccg gcaatggcga tcatgtagcc gcccgatgcc gcgacatctt cggtgaagac
     6121 gaggacgcgc ttgttctttt caaccgccag gtcgcgaatg cgctggtaga tcagccgcga
     6181 ttgcaccggc gagccgcccg gcgagttgat cgagatggcg acggccggcg cgtcgaaggc
     6241 aaacgccttt tcgatcaggc cggcggtcga agccagcgac aggctcggc
//
LOCUS       DASYCS010000360         4616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2133455,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000360 DASYCS010000000
VERSION     DASYCS010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4616
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2133455"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VGM46_11295"
     CDS             <1..235
                     /locus_tag="VGM46_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018235376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEY4193219.1"
                     /translation="EGVRACYETLTSREREVMALVVTGRLNKQIGADLGISEITVKVH
                     RGQVMRKMHASSLPDLARMADKLQLKPEKAQTT"
     gene            complement(327..1283)
                     /locus_tag="VGM46_11300"
     CDS             complement(327..1283)
                     /locus_tag="VGM46_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009494020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEY4193220.1"
                     /translation="MTTHSLTATPTTVRRGMFDGSFSPVLTVQPGDTVVLQSVSGAPH
                     VMPPPEAGFAVPKALAEILAAKLPSLGSHLVTGPVAIAGAEPGDMLEVCIDAIDLGAD
                     WGYCAFHPLIGSLPEDFPFRDVSYIPVDVAKKTCKLPWGPELPLAPFFGVMAVAPPLV
                     YGTVSTREPREFGGNLDNKELIAGSTLYLPVWVSGANFSAGDGHGVQGDGEVCINALE
                     ICLTGTFTFKLHKGGGANAPLLRIPRAETPTHYISMGLNADLDLAMKQALREMIAFIC
                     ERTGWSPAQAYKSCSLAVDFRVTQNVNGEKGVHGLLKKGLLF"
     gene            complement(1448..2407)
                     /locus_tag="VGM46_11305"
     CDS             complement(1448..2407)
                     /locus_tag="VGM46_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HEY4193221.1"
                     /translation="MRIAVIDDYQDAFRSLDAATKLKGHDVKVFHAMAKDDQALIDML
                     KGFEAVVLIQQRTAMPRKVIEALPDLKMISQTGKNTAHIDMAACKERGIVVSAGGGGT
                     PNATAEIAWSLILSCARNIPNEVQAMKEGKWQISLGNVLAGKTLGVYALGKIGTVVAT
                     VGKAFGMKVVCWGREGSLARAKEAGFEVAASREAFFSQCDVISLHIPLNKETTGIVTA
                     ADLALMKPESILVNTSRAGLIEKGALVAALKAGRPGRAGIDVYETEPVLNGDHPLLKL
                     PNAVCTPHLGYVEKATYEALYGAAADQIVAYAAGKPINVVTPA"
     gene            complement(2456..3433)
                     /locus_tag="VGM46_11310"
     CDS             complement(2456..3433)
                     /locus_tag="VGM46_11310"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY4193222.1"
                     /translation="MSVRSLLAAMAISLAFGAVAKAQDYPNRPITIIVPYTAGGGADV
                     VARITADGLERQLKQRVIIENVTGAGGTTGIRRAANAAPDGYTMMTVSPGTHSAAPAL
                     YKDLGYDPIKSFEMVGLTATQPLVLIVRKELPVSNLKEFVTYLKAHEKTVTLAHVGPG
                     SMTHLGCTMFDALVGVNPTAAPYRGTPPLMQDLLGGRVDYTCQQPNGVIGFVQKGEVK
                     ALAVAGDKRMPSLPNVPTSDEAGMPDFKSSAWNALAMPKGVPDAIVNKVNAALNKALN
                     DPDVIKRFATLDLVVPPPEERSREYMLKFMKIELDRYVDLMRKAGVEPQ"
     gene            complement(3527..4495)
                     /locus_tag="VGM46_11315"
     CDS             complement(3527..4495)
                     /locus_tag="VGM46_11315"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEY4193223.1"
                     /translation="MDAIVIEQFGDASVLQFKTRETPQPNADQIAIKVSVTSVNFADT
                     IWRNGRPGAKLPFTPGLDCAGTVLAVGANVKEFKIGQRVAAFVDGGSYADVTLARPVL
                     TYAIPDGVSDDDACGLVMLVTGWNLVKMASAGNMKAGDNLLVHAGAGGVGSIAIQVAR
                     NFGAGKIIATVGDMAKADYARQCGADVVLRANDFVNGTKEATGGKGVDIILDSVAGSG
                     FPGSMEVLAPYGRYVNYGNAGGAPGVVKTSELHANNRSVIGYSSGHYRANRPEALRPS
                     IEGCFKAIADKKLKVLVSKTFPMKEAAESHRFIESRKSFGKILLKP"
BASE COUNT          894 a         1306 c         1466 g          950 t
ORIGIN      
        1 ggaaggagtg cgcgcgtgtt acgaaacgct gacctcgcga gagcgcgaag tcatggcttt
       61 ggtggtgact ggccggctta acaagcagat cggtgcagac ttagggatca gcgaaataac
      121 ggtcaaggtt caccgcggtc aggtgatgcg caagatgcac gcttcgtcat tgcctgactt
      181 ggcacgaatg gccgacaagc tgcagctgaa accggaaaag gcccaaacga cataacccca
      241 agaacgcagc cgccccttaa agttcatccc tatgcattgc taaaaagact ctgggttttc
      301 acgcgcgtat ctgaagttaa gaccactcaa aacagcaaac ctttcttgag caggccgtga
      361 acgcctttct cgccgttgac gttctgcgtc accctgaagt cgacggcgag agaacagctt
      421 ttgtaagctt gggccggact ccatccggtc cgttcgcaaa taaacgcaat catttcccgc
      481 aacgcctgtt tcatggcgag gtccagatcc gcgttgaggc ccatgctgat ataatgggtg
      541 ggggtctccg cccgtgggat acgcaataac ggagcgttag cgccgccgcc tttgtgaagc
      601 ttgaaggtaa aagtgccggt gaggcagatt tcgagtgcgt tgatacagac ctcgccgtcg
      661 ccctgcaccc cgtggccgtc gccagccgag aagtttgcac cggataccca gacgggcagg
      721 tagagtgtgc tgccggcaat cagttccttg ttgtcgaggt tgccgccgaa ttcccggggt
      781 tcgcgtgtcg aaacggtgcc gtaaacaaga ggcggcgcca ccgccatcac gccgaaaaag
      841 ggtgccagcg gcaattccgg gccccacggt aatttgcagg tcttcttcgc aacgtccacc
      901 gggatgtagc tgacatcgcg gaagggaaag tcttccggca ggctgccgat gagggggtgg
      961 aaggcgcaat agccccagtc ggcgccgaga tcgatggcgt cgatacagac ttcaagcata
     1021 tcgcccggct cggcgccggc gatggcgacc ggaccagtga caagatggct accaaggctc
     1081 ggtagcttgg cagcgaggat ttcagcgagg gctttcggga ccgcaaaccc ggcttccggc
     1141 gggggcatga cgtgaggcgc gccagagacc gattgcagca ccacggtgtc gcccggctgg
     1201 accgtgagga cgggagagaa cgagccgtcg aacatgcccc ggcgaacagt tgttggcgtc
     1261 gctgtcaatg aatgggtggt cattttctct gctccaagcg taagacggcg atgctatcgg
     1321 tactgtcgcc cttagcgtac gatttcccgc gctcagataa caaaaaaaaa tcgagacaga
     1381 ccgctgcccg ttctcctgtt gcgatacccc gagcggcttg gccgcccggg gtagccgcta
     1441 cggagtttta cgccggagtg acgacgttga tcggcttgcc ggcggcatag gccacgatct
     1501 gatcggcggc ggcgccgtac aaggcctcat aggttgcctt ctcgacgtag ccgaggtgcg
     1561 gcgtgcagac ggcattcggc agtttgagca gcggatgatc gccattgagc accggctcgg
     1621 tctcgtagac gtcgatgccg gcacgtcccg gacgacccgc cttcagggcc gcgaccaacg
     1681 cacctttctc gatcaggccg gcgcggctgg tgttgaccag gatcgactcc ggcttcatca
     1741 gggcgagatc ggcggcagtg acgatgccgg tcgtttcctt gttgagcggg atgtgcagcg
     1801 agatcacgtc gcactgagag aaaaaggctt cgcggctggc ggcgacctcg aaaccggctt
     1861 ccttggcgcg ggcgagggag ccttcacggc cccagcaaac gactttcatg ccgaaggcct
     1921 tgccgacggt ggcgacgacg gtgccgatct tgcccagcgc gtagacgccg agcgtcttgc
     1981 cggcgagcac gttgccgagc gagatctgcc acttgccttc cttcatggcc tgcacttcgt
     2041 tcgggatatt gcgtgcgcag gacagaatga gcgaccatgc gatttccgcc gtcgcgtttg
     2101 gcgtgccgcc gccgccggcc gatacaacga tgccgcgttc cttgcaggcc gccatgtcga
     2161 tatgggccgt gttttttccg gtctggctga tcatcttcag gtccggcaac gcttcgatca
     2221 ctttgcgcgg catcgcagtg cgctgctgga tcagcacaac ggcctcgaaa cccttgagca
     2281 tgtcgatgag ggcctggtcg tctttggcca tggcatgaaa caccttcacg tcgtggccct
     2341 tcaatttggt ggcggcgtcc aaagagcgga aagcgtcctg atagtcgtca atcacagcga
     2401 tacgcattaa ggcattcctt gttctggttt ggagcattgc catgttcaat agagcttact
     2461 gcggttcgac gcctgccttg cgcatcaaat cgacgtagcg gtcgagttcg atcttcatga
     2521 acttcagcat gtattcgcgg gaacgttctt ccggtggcgg cacgacgaga tcgagcgtgg
     2581 cgaagcgctt gatgacatcc ggatcgttca gcgccttgtt gagcgccgca ttgaccttgt
     2641 tgacaatggc gtcaggcacg cccttcggca tggcgagcgc attccaggcg gaggacttga
     2701 aatcgggcat gccggcttca tcggatgtcg gcacattggg caaagagggc atacgcttgt
     2761 cgccagcaac ggcgagtgct ttcacttcgc ctttttgcac gaagccgatg acaccgttgg
     2821 gctgctggca ggtgtagtcg acacggcccc caagcaaatc ctgcatcagt ggcggcgtgc
     2881 cgcgatacgg cgcggcggtc gggttcacgc cgacaagggc gtcgaacatt gtgcagccca
     2941 gatgcgtcat ggaaccgggg ccgacgtggg cgagggtgac ggtcttttcg tgggccttga
     3001 gataggtgac gaactccttc aggttgctga ccggcagttc cttgcggaca atgagcacga
     3061 gcggctgggt ggccgtgagg ccgaccattt cgaagctctt gatcggatcg tagccgagat
     3121 ccttgtagag cgcgggcgca gcggaatgcg tgccgggcga cacggtcatc atcgtgtagc
     3181 catctggagc ggcgttggcc gcgcggcgga tgccggtcgt gccgccggcg ccggtcacgt
     3241 tttcgatgat gacgcgctgt ttgagttgac gctcaagacc gtcggccgtg atgcgggcga
     3301 cgacgtcggc gccgccaccg gcggtgtaag gcacgatgat cgtgatgggc ctatttggat
     3361 agtcttgcgc cttggcaacg gcaccaaatg ccagcgagat ggccatggcg gcaagcagtg
     3421 aacgtacgga catagtcccc tccatttgtg gtttgtgccg cttttctgac gcgcggcttg
     3481 atgttcaaaa tgtttggcgg cgcgaatttc gcgccgccaa cagcggttac ggcttgagaa
     3541 ggatcttgcc gaagctcttg cgtgactcga tgaagcggtg cgactcggcc gcttctttca
     3601 tcgggaaagt tttgctgacg agcaccttga gcttcttgtc ggcaatggcc ttgaagcagc
     3661 cttcgatcga aggccgcagt gcttccggcc gattggcgcg atagtggccg ctcgaatagc
     3721 cgatgaccga gcggttattg gcgtgcagtt cgctggtctt cacgacgcca ggtgcgccgc
     3781 cggcgttgcc gtagttcacg tagcggccgt agggcgcgag aacttccatg ctgccgggga
     3841 agccggagcc ggcaaccgag tccagaatga tgtcgacgcc cttgccgcca gtcgcttcct
     3901 tggtgccgtt gacgaagtca ttggcgcgca ggacgacatc ggcgccgcat tggcgggcgt
     3961 agtcggcctt cgccatgtcg ccgacagtcg cgatgatttt gccggcgccg aagttcctgg
     4021 cgacctgaat agcgatggag ccgacaccgc cggcgcccgc gtgtacgagg aggttgtcgc
     4081 ccgccttcat attgcctgcg ctggccattt tgacgagatt ccaacccgtc acgagcatca
     4141 caaggccgca agcatcgtcg tcactgacgc cgtccggaat ggcataggtc agaacagggc
     4201 gggcaagtgt aacgtctgca taggagccgc catcgacgaa ggccgcaacg cgctggccga
     4261 tcttgaattc cttgacgttg gcaccgaccg ctaaaaccgt gccggcacaa tcgaggccgg
     4321 gcgtgaacgg tagtttggcg ccggggcggc cattacgcca aatcgtgtcg gcgaagttga
     4381 cgctggtgac ggaaaccttg atggcgatct gatcggcgtt gggctgcggc gtttctcgcg
     4441 ttttgaactg cagtaccgac gcatcgccaa attgttcgat gacgattgca tccatagtat
     4501 tgtcctccgt gaagaatatt ttcactgctt tacctgcaaa cgaaagacga atgtcgggtg
     4561 caggagtctg aatattctga agtcaacttt cagaaattag aagtatgtac tttgga
//
LOCUS       DASYCS010000361         2473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3290334,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000361 DASYCS010000000
VERSION     DASYCS010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3290334"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="VGM46_11320"
     CDS             <1..911
                     /locus_tag="VGM46_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY4193224.1"
                     /translation="LAKVPMLRGRVMALNGVDVAKVKVPAEGGWVLKGDRGLTYDAKK
                     PENTTLSEGEWWPADYAGEPLVSFSAEEARQIGLKLGDTVTVNVLGRNITAKIANFRQ
                     VEWETMGINFVMVFSPNTFAGAPHAWIATLADKQASVADDARVLNAVARQFPTITTVR
                     VKDALDIVNRLVGELGTAIRAAASVALIASVLVLAGALAAGNRARIHDAVVLKTLGAT
                     RRTLIAAYSLEYALIGLATAVFALVAGGLAAWFVIARIMKLPSHFSPEVALATVILSL
                     GVTVGLGLLGTWRVLGQKAAPVLREL"
     gene            1141..1920
                     /locus_tag="VGM46_11325"
     CDS             1141..1920
                     /locus_tag="VGM46_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEY4193225.1"
                     /translation="MADLRNYQTRAVPAGTRADADIDQGLRAYMIKVYNLMGLGLLIT
                     GLAAAATVMLATTTDPSAAVATLPGGEMLTSFGYAIFASPLKWVVILAPLAAVMFLSF
                     RVQSMSVSAAQTTFWVYAGLVGLSLSSIFLVYTTASITQTFFATAAGFGALSLFGYTT
                     KRDLTAMGSFLIMGVFGLIIAMVINIFLQSSGLSFAISAIGVLIFAGLTAYDTQKIKE
                     MYFEGDDATVAGRKSIMGALTLYLDFINLFMFLLQFMGDRR"
     gene            2036..>2473
                     /locus_tag="VGM46_11330"
     CDS             2036..>2473
                     /locus_tag="VGM46_11330"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY4193226.1"
                     /translation="MSKLLTRPAILADMARITEIYTDAVLNGTASYEIDPPTQAVMTE
                     RFEVLVGQEYPYIVAEGSDGTLLGYAYASAFRPRHAYRFMAEDSIYVAPEAKGRGVGR
                     LLLEALVEATQALGFRQMIAVIGDGRPDSPSVRLHERLGFHHSG"
BASE COUNT          425 a          819 c          737 g          492 t
ORIGIN      
        1 cgctggccaa ggtgccgatg ctgcgtggcc gcgtcatggc gctgaacggc gtcgatgtcg
       61 ccaaggtgaa ggtgccagcc gaaggcggct gggtgctgaa gggcgaccgc ggcctgacct
      121 acgacgcgaa gaagcctgaa aacaccacgc tgagcgaagg cgaatggtgg ccggccgact
      181 atgccggcga gccactggtt tcgttttccg ccgaggaggc gcgccagatc ggcctcaagc
      241 ttggcgacac cgtcaccgtc aacgtgcttg gccgtaatat taccgcgaaa atcgccaact
      301 tccgccaggt cgaatgggaa accatgggca tcaatttcgt catggtcttt tcgcccaaca
      361 ccttcgccgg cgcgccccat gcgtggatcg ccacgctggc cgacaagcag gcttccgtcg
      421 ccgatgacgc ccgcgtgctc aacgccgtcg ccaggcagtt cccgaccatc accacggtgc
      481 gggtcaagga cgcgctcgat atcgtcaacc ggctggtcgg tgagctcggc accgccatcc
      541 gcgcggcggc gagcgtggcg ctgatcgcct cggtgctggt gctggccggc gcgctggccg
      601 ccggcaaccg cgcccgcatc cacgatgcgg tcgtgctgaa gacgctgggc gccacaaggc
      661 gcacgctgat tgccgcctat tcgctggaat acgcgctgat cgggctggcc accgccgtgt
      721 ttgcgcttgt cgccggcggc ctcgcggcct ggttcgtcat tgcgcgcatc atgaaactgc
      781 cgtcgcattt ttcgccggaa gtcgcattgg caaccgtcat cctgtcgctt ggcgtaaccg
      841 tcggccttgg cctgctcggc acctggcgcg tgcttggcca gaaggccgcg ccggtgctgc
      901 gcgaactgtg acaagcgccg cgatttgggg cgcaaggcaa tcttttctcc gctccgtttg
      961 accgccaaag cgaaaaatat gccgcgctgc ccttggttca cgaagcattg caatccgtca
     1021 cggtcttgtt cttgacggaa agaaccatca tattgcgcca taggcatgct ggagtcccgc
     1081 cagcggcgcc tgggccgaaa agcccgacac cggacgggac agaagcaaat agaggaaaca
     1141 atggctgatc tgcgtaacta tcagacgcgc gccgtgcctg ccggcacccg tgcggatgcc
     1201 gacattgatc agggcctgcg cgcctacatg atcaaggtct ataatttgat ggggcttggc
     1261 ctcctcatca ccggccttgc cgccgccgcc accgtcatgc tggcgacgac caccgatcca
     1321 tccgccgccg tcgcaaccct gccgggcggc gaaatgctga cctccttcgg ctatgcgatc
     1381 ttcgcctcgc cgctcaaatg ggtcgtcatc ctggcgccgc tggccgccgt gatgttcctg
     1441 tccttccgtg tgcagtcgat gagcgtttcg gccgcgcaga ccaccttctg ggtctatgcc
     1501 ggcctggtcg gcctgtcgct gtcgtcgatc ttcctggtct atacgaccgc cagcatcacg
     1561 cagaccttct ttgccaccgc cgccggcttc ggcgcgctgt cgctcttcgg ttacaccacc
     1621 aagcgtgacc tgaccgcgat gggttcgttc ctgatcatgg gcgtgttcgg cctcatcatc
     1681 gccatggtca tcaacatctt cctgcaatct tctggcctct ccttcgccat ctcggcgata
     1741 ggcgtgctca tcttcgcggg cctcaccgcc tacgataccc agaagatcaa ggagatgtat
     1801 ttcgaaggcg acgacgcaac tgtcgccggc cgcaagtcga tcatgggcgc gctgacgctt
     1861 tatctcgact tcatcaacct gttcatgttc ctgctccagt tcatgggcga ccgccgctag
     1921 gcgctctcat ccgctggctg gatcagcata aatcgcaagg gcggcccaac ggccgccctt
     1981 ttcttttgcg cccagcttgc tctaatatga ggcatcgaaa actggtgcct aaattatgag
     2041 caaactactg acccgccccg ctatattggc ggatatggcc cggatcaccg agatttatac
     2101 cgacgctgtc ctcaatggca cggccagcta tgaaatcgac ccgccgacgc aagccgtgat
     2161 gaccgagcgc ttcgaagttc tggtcggcca ggaatatccc tacatcgttg cggaaggcag
     2221 cgacgggacg ctcctcggtt acgcctatgc cagcgccttt cgcccgcgcc acgcctatcg
     2281 cttcatggcc gaggattcga tctatgtcgc gccggaggcc aagggccggg gtgtcggccg
     2341 cctccttttg gaggcgctgg tcgaagcgac gcaggcgctg ggctttcgcc agatgatcgc
     2401 cgtcatcggc gacgggcggc ccgacagccc atcggtaagg ctgcatgaaa ggctgggctt
     2461 tcatcacagc ggc
//
LOCUS       DASYCS010000362         3407 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4740249,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000362 DASYCS010000000
VERSION     DASYCS010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4740249"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..559)
                     /gene="flgA"
                     /locus_tag="VGM46_11335"
     CDS             complement(50..559)
                     /gene="flgA"
                     /locus_tag="VGM46_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HEY4193227.1"
                     /translation="MLWPASRSALRNAAIVVALLCACAPAIGQENTAVAVSQTGGEVV
                     LIPNRVIYPGEIVALGALKEVTLVPGRKKPDAVATLIGELDGKIAKRTLLPGHYIPVN
                     ALRDPWVVEQGAPVQVIFASGPLTISAAAVTLQPGSTGDLVKVRNLDSGKIFSGTVMA
                     DGTIRVGAS"
     gene            complement(565..1353)
                     /gene="flgG"
                     /locus_tag="VGM46_11340"
     CDS             complement(565..1353)
                     /gene="flgG"
                     /locus_tag="VGM46_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018324173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HEY4193228.1"
                     /translation="MRALAIAATGMNAQQTNLEVIANNIANINTTGYKRARAEFSDLL
                     YQVDRTQGVPNRANGSLVPEGVSIGLGVKTSAVRNVHTQGELTSTGNAFDMALTGKGW
                     FQVEAADGSTLYSRAGAFNTNADGQLVTVDGATVIPEITVPQDAVEVIVNKTGQVFGR
                     IDGQTDLQDLGQLTLANFANEAGLAPMGDNLFQETPASGPANVGIPGDPGYATIEQGY
                     LEASNVDPVKEITELISAQRAYEMNSKVIQAADDMASVVSKNIR"
     gene            complement(1364..1690)
                     /locus_tag="VGM46_11345"
     CDS             complement(1364..1690)
                     /locus_tag="VGM46_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HEY4193229.1"
                     /translation="MIGGISALQFKPAIGGADVTTGLLSGGAAPVAGGDIASSFSQAL
                     ADAANKTVGTMQNAEHLSIQGLKGDVDTREVVDAVMNAQQALQTAVAIRDKIVSAYLD
                     VSRMSI"
     gene            complement(1693..2106)
                     /gene="flgC"
                     /locus_tag="VGM46_11350"
     CDS             complement(1693..2106)
                     /gene="flgC"
                     /locus_tag="VGM46_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011974466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HEY4193230.1"
                     /translation="MDALTSALKVAASGLGAQSERLRVVSENLANAQSTGTTAGSDPY
                     RRKTISFVSELDRASGASLVDVGSIDRDHSDFPVEFQPGNEAADEKGYVKMPNVNVLI
                     EMADMQEANRSYEANLQVVKQARDLISMTIDLMRS"
     gene            complement(2109..2489)
                     /gene="flgB"
                     /locus_tag="VGM46_11355"
     CDS             complement(2109..2489)
                     /gene="flgB"
                     /locus_tag="VGM46_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HEY4193231.1"
                     /translation="MEPVNLFGLATKQAQWLAVRQSSIAGNIANANTPGYTATDVQPF
                     EKVLDQTAVSLRATQAGHLGSGATDASFAIKQQDYTGSIMPSKNTVVVENELLKAGEV
                     RRGFELNTAIVKAFHSMMMSVVKG"
     gene            complement(2573..3319)
                     /locus_tag="VGM46_11360"
     CDS             complement(2573..3319)
                     /locus_tag="VGM46_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193232.1"
                     /translation="MPNPETERQRTEQVLRELLAEAQAAEGAVEESRDFLLSGLFSKN
                     PADTAANRPQDDRSRKRKPGKDDMEWPQPPQAQFPKPAAKADRRSDLVVAALGITLGL
                     ICAMFPWYIFYNQVQFHTHGFRLGGNGERSGRIVTSAEPRGDAAPLSEKDLPMQKLDL
                     FATGTPAESRQDPAKTPGLDEQPFPAGNDDFRLVHVANGRAMIEDDAGVWIVQAGSTL
                     PDNSRVTSIEKRAGKWVLVTSKNQVVGLTQ"
BASE COUNT          581 a         1006 c         1138 g          682 t
ORIGIN      
        1 gggtgcaggc aaagcaccgc cattgccaaa gaaaggacga ggccacgctt catgacgcgc
       61 cgacccggat ggtgccgtcc gccatgaccg tgccggagaa tatcttgccg ctgtcgaggt
      121 tgcggacctt gaccagatcg cccgtcgagc cgggctgaag cgtcacggcg gctgctgaaa
      181 tggtcagcgg gccggacgcg aatatgacct gaaccggcgc gccctgctcg accacccacg
      241 gatcgcgaag ggcgttgacg ggaatatagt ggccgggcag cagggtccgc ttggcgattt
      301 tcccgtcaag ctcgccaata agggtggcaa cggcatccgg ttttttcctt cccggaacca
      361 gcgttacttc cttcagcgcg ccaagggcga cgatttcgcc gggatagatc acccggttgg
      421 ggatcagcac gacttcgccg ccggtctggc tgacggcgac agcggtgttt tcctggccga
      481 ttgccggcgc gcaggcgcaa agcagcgcga cgacaatcgc ggcattgcgc aatgcagagc
      541 gggaagccgg ccaaagcacc ttcgtcacct gatgttcttg gagacgaccg aggccatgtc
      601 gtcggcggcc tggatgacct tggaattcat ctcataggcg cgctgcgccg atatgagttc
      661 ggtgatttcc ttgaccggat cgacgttgga agcttcgaga tagccctgct cgatggtggc
      721 atagccggga tcgccgggga tgccgacatt ggcggggccg gaggccggcg tttcctggaa
      781 aagattatcg cccatcggcg ccaggccggc ctcgttggcg aaattggcga gcgtcagctg
      841 gccgagatct tgcaggtcgg tctggccatc gatgcggccg aacacctggc cggtcttgtt
      901 gacgatgact tccacggcgt cctgcggaac ggtgatttcc gggatgacgg tcgcgccatc
      961 gacggtcacc agctggccat cggcattggt gttgaaggcg ccggcgcggc tatagagcgt
     1021 cgagccatcg gcggcttcga cctggaacca gcccttgccg gtcagcgcca tgtcgaaggc
     1081 gttgccggtg ctggtcagtt cgccctgcgt atggacgttg cgcacggcac tggtcttgac
     1141 gccaaggccg atggagacgc cttccggcac cagcgaaccg ttggcgcggt tgggcacgcc
     1201 ctgcgtgcgg tcgacctgat agagcaggtc ggagaattcc gcccgcgccc gcttgtagcc
     1261 ggtggtgttg atgttggcga tattgttggc gatgacttcc agattggtct gctgggcgtt
     1321 catgccggta gctgcgatgg cgagtgctct cacggggaat tcctcaaatg ctcatgcggc
     1381 tgacgtcgag ataggcggag acgatcttgt cgcgaatggc caccgccgtc tgaagcgcct
     1441 gctgggcgtt catcacggcg tccaccactt cgcgggtgtc gacatcgccc ttcagcccct
     1501 ggatcgagag atgctcggca ttctgcatcg ttccaacggt cttattggcg gcgtcggcca
     1561 gcgcctgcga aaaagaagaa gcgatatcgc caccggcaac cggtgcggct ccgccggaaa
     1621 gcaggccggt cgtaacgtcg gcgccgccaa ttgccggttt gaattgaaga gcgcttatgc
     1681 cgccgatcat ccctagctcc tcatcaaatc tatggtcatg gaaatcaggt cgcgcgcctg
     1741 cttgacgacc tgcaaattgg cttcatacga ccggttggct tcctgcatgt cggccatctc
     1801 gatcagcacg ttgacgttgg gcatcttgac gtagcccttt tcgtcggcgg cctcattacc
     1861 gggctggaac tcgaccggaa agtcggaatg atcgcggtcg atcgagccga catcgacgag
     1921 ggaagcgccg gaggcgcggt ccagttccga aacgaagctg atggtcttgc gccgataagg
     1981 gtcggaaccg gccgtggtgc cggtcgactg ggcgttggca aggttttccg agacgacgcg
     2041 caggcgctcg gactgggcgc cgaggccgga ggctgcaacc ttgagggcgg aggtgagcgc
     2101 gtccatggtc agcccttcac cacgctcatc atcatggaat gaaacgcctt gacgatcgcc
     2161 gtgttcaatt cgaagccgcg acggacttcg ccggctttca gcagctcgtt ctcgaccacg
     2221 acggtgtttt tcgaaggcat gatcgagccg gtgtagtcct gctgctttat ggcgaagctg
     2281 gcgtcggtcg cgccgctgcc gagatggccg gcctgcgtgg cgcgcagcga aacggccgtc
     2341 tggtcgagga ctttctcgaa aggctgcaca tcggttgcgg tatagccggg cgtattggca
     2401 ttggcgatgt tgccggcaat ggaagactgg cgcacggcga gccattgcgc ctgcttggtt
     2461 gcaagaccga aaaggttgac gggctccata gaaatctccg ggcggatcag cggagactac
     2521 ggcgtcagtc ttgcgtggac cttgcggagg cttttcccgt aaagcggggc gattattgcg
     2581 tcagcccgac gacctggttc ttgctcgtca caagcaccca tttgccggcg cgcttttcta
     2641 tcgaggtgac gcggctgttg tcgggcagcg tcgatcccgc ctggacgatc cagacaccgg
     2701 cgtcgtcctc gatcatggcg cggccattgg cgacatgcac gaggcggaaa tcatcgttgc
     2761 ccgccggaaa aggctgctcg tcgaggccgg gcgtcttggc tgggtcctgc cggctttcgg
     2821 cgggcgtgcc tgtggcgaac agatcgagtt tctgcatggg caggtctttt tcggacaggg
     2881 gcgcggcgtc gccgcgcggc tcggccgaag tgacgatgcg gccgcttctt tcgccattgc
     2941 cgccgagccg gaagccatgc gtgtggaact ggacctgatt atagaaaatg taccatggaa
     3001 acatcgcgca gatcaggccg agcgtgatgc cgagcgccgc cacaaccaga tcgctgcgcc
     3061 ggtcggcctt ggccgccggt ttcgggaact gcgcctgggg tggctgcggc cactccatgt
     3121 cgtccttgcc gggcttacgc ttgcgggaac ggtcgtcttg cggcctgttt gcggcggtgt
     3181 cggcagggtt cttcgaaaaa agccccgaaa gcaggaagtc gcggctttcc tcgacggctc
     3241 cctccgctgc ctgcgcctcg gccagcaatt ctcgcaggac ctgctcggtg cgctgccgct
     3301 cagtctccgg attcggcatt ctgcttgacc ttcagatggc gggcgagatc ggagaatgga
     3361 tcggccgaag ggcggtcttt cggcgattgc gtcaacgcct cgtagat
//
LOCUS       DASYCS010000363         3003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1409693,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000363 DASYCS010000000
VERSION     DASYCS010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1409693"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="VGM46_11365"
     CDS             complement(<1..338)
                     /locus_tag="VGM46_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEY4193233.1"
                     /translation="MAETIRIIGIDPGLRRTGWGVVESLGNSLRFLASGTVKSDDKAA
                     LAYRLCQLHDGLAEVLHAAMPHEAAVEQTFVSKDASATLKLGQARGIAMLVPARAGLT
                     VAEYAPNAVK"
     gene            complement(417..950)
                     /locus_tag="VGM46_11370"
     CDS             complement(417..950)
                     /locus_tag="VGM46_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1465 family protein"
                     /protein_id="HEY4193234.1"
                     /translation="MNELSRESVKTIKLAERRVFSQSFKPLYDEGMGLVEQTAEYLDG
                     QGRAEARKLSRLGATLYAAESMRLTTRLMQIASWLLLQRAANSGEMTRDQVASEKSKV
                     RLDTASAHEDAVGWDELPDAFLELVTHSLRLQALVRRMDEEIYGSGPLTSIEPGARRL
                     NPVSDQISLLNTAFARG"
     gene            complement(1296..1679)
                     /locus_tag="VGM46_11375"
     CDS             complement(1296..1679)
                     /locus_tag="VGM46_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193235.1"
                     /translation="MQGVARNFFTLAIVYALCGMALGLHMGISQDHSQMPTHAHIMVA
                     GWLMSAVFAFFYHLFPTIAAKMLAKVHFWLTAISGIGLMIGLFYEFGGSEAMEPVLAI
                     ASMGFYAGMILFAVIALPAIWRPAV"
     gene            2046..2693
                     /locus_tag="VGM46_11380"
     CDS             2046..2693
                     /locus_tag="VGM46_11380"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEY4193236.1"
                     /translation="MNETTPPNRCRIVLIAPPAASAERLRAAFDGGDVASLILPQYDM
                     DEMAFQALAEQVVPAAQARGAAVIIAGDTRIAGRVQADGLHVEAGKAELAETIGNFQS
                     KMMVGCGGAKTRNDALELGETQPDYIFFGRFGYDNKPEAHSRNLSLGQWWAEMIEIPA
                     IVMAGSDIASVETVAATGCEFVALSSAVFAEGVDAREAVSRANALLDQTAPRFEG"
     gene            2696..>3003
                     /locus_tag="VGM46_11385"
     CDS             2696..>3003
                     /locus_tag="VGM46_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193237.1"
                     /translation="MPARQLLIAGLLAAMTLPALATEPATPSPAMQPSAPAPIQVSPV
                     QPPPARPALPQSQRVTSPTADRLDRPIAKPLPQPATTAEPGPLPSIDAINPERFGARP
                     A"
BASE COUNT          592 a          937 c          930 g          544 t
ORIGIN      
        1 ttcttgacgg cgttgggcgc atattcagcc accgtcagtc cggcgcgggc cgggaccagc
       61 atggcgatgc cgcgcgcctg gccaagtttc agcgtggcgg aagcgtcctt gctgacgaag
      121 gtctgttcca ccgccgcttc atggggcatg gccgcatgca acacttcggc cagaccgtca
      181 tgcaactggc agagcctgta ggcaagcgct gccttgtcgt ccgacttcac cgtaccggag
      241 gcgaggaagc gcaacgaatt gccaaggctt tcgacgacgc cccagcctgt gcggcgaagc
      301 cccggatcga tgccgatgat gcgaatcgtt tccgccatgc gcgaagtcta cctttcgcgg
      361 caagcgatgc cagcaaaaag tgaacaaacc agaaacgacg taggttgcag gcggcctcag
      421 ccgcgcgcga acgccgtgtt gagcagcgag atctggtcgg ataccggatt gaggcggcgc
      481 gcgcccggtt cgatcgaggt taagggaccg gagccgtaga tttcctcgtc catgcgccgc
      541 accagcgcct gcaagcgaag cgaatgggtg acgagttcga ggaacgcgtc gggaagttcg
      601 tcccagccga cagcatcctc atgcgcggaa gccgtgtcga gccgcacctt ggacttttcc
      661 gaagcaacct gatcgcgcgt catctcaccg gaattggcgg cgcgctggag caggagccac
      721 gaggcgatct gcatcaggcg ggtagtcagc cgcatggatt ccgcggcgta gagggtggcg
      781 cccagccgcg acagcttcct ggcctcggcg cggccctggc catcgagata ttcggcggtc
      841 tgctcgacca gccccatgcc ttcatcatag agcggcttga acgattggga aaaaacacgg
      901 cgctcggcca gcttgatcgt tttcacgctt tccctcgaaa gttcattcat cgctgcgccc
      961 tattgcaccg gccgaatcag gtccatccga tacgccggct tcctatacaa tccaggccgg
     1021 aaaaatgcgc tttgggcgtg aggttggcaa ggacatgttt aataaagggt taacgggcga
     1081 agggtgcttc aggagacaaa aaaagagccg cggatgaggc ggctctcagg agtttaacag
     1141 ggaggcgtca aacaaagtgg ctccaaccac ttggtaagaa tccagataac tggatgaaca
     1201 tagtaaacac ctgtaaagct taacgaagcg ttaaaggcgc cggttttatt caatattgct
     1261 gaagttcatg ggccgccggt ccagcgctct tttcgtcaga ccgccggccg ccagatcgcc
     1321 ggcaacgcaa tgacggcgaa caggatcatg ccggcgtaaa agcccatcga ggctatcgcc
     1381 agcaccggct ccatcgcttc gctgccgccg aactcgtaaa acaggccgat catgaggccg
     1441 atgccgctga tggcggtcag ccagaaatgc accttcgcca gcatcttggc ggcaatggtg
     1501 ggaaacaggt ggtagaagaa ggcgaagacc gccgacatca gccagcccgc caccatgata
     1561 tgagcgtggg tcggcatctg actgtgatcc tggctgatgc ccatatgcag gccaagcgcc
     1621 ataccgcaca gcgcgtagac aattgccagc gtgaaaaaat tccgtgccac tccttgcatg
     1681 cggtcccctc catttgcctt ttccctgtcc gggaatgggt ttcagtaagc gtgaggcgcc
     1741 gccgaatgcg ccgcttccag gccgtatcgg gcatgatgca gcggctgcgg ctcccccagc
     1801 caccaagcta cattagcaga aaatgatgcg gcggctttcg ccgatggcgg gattcaccgg
     1861 ttaatttaag gcccacgggg catttttggc agccattcaa tttcgtttcg tcatttttcg
     1921 tagaagcgag tggctggccg catctcatcc tgtttggcgc gcatcttctc aaggctggcg
     1981 ctttgctcta tgccgcaatg caaccatctt cgcccggcaa taggcgaaaa ccgggattgg
     2041 aaaccatgaa cgagacaacg cccccaaacc gctgccgcat cgtcttgatc gcgccgcccg
     2101 ccgcttcggc ggagcgcctg cgcgcggcgt tcgatggcgg cgacgtggcg tcgctgatcc
     2161 tgccgcaata cgacatggac gaaatggcgt tccaggcgct ggcggagcag gtcgtgccgg
     2221 cggcgcaggc caggggcgcg gccgtcatca tcgccggcga cacgcgcatc gcgggccgtg
     2281 tccaggccga tggcctgcat gtggaagcgg gcaaggcgga actggccgag acgatcggga
     2341 atttccagtc gaagatgatg gtcggctgcg gcggcgccaa gacccgcaac gacgcgctgg
     2401 agcttggcga aacgcagccc gattatattt tcttcggccg cttcggctat gacaacaagc
     2461 cggaggcgca ttcgcgcaat ctgtcgcttg gtcagtggtg ggcggaaatg atcgaaattc
     2521 cagccatcgt catggccggc tccgacatcg cctccgtcga aacggtcgcg gctaccgggt
     2581 gcgagttcgt ggcgctttca agcgcggtgt ttgccgaagg cgtggatgcg cgcgaggcgg
     2641 tctcgcgcgc caacgcgctg cttgaccaga cagcaccgcg cttcgagggg tgacgatgcc
     2701 cgcgcgccag ttgctgattg ccgggctgct tgccgcgatg actttgccgg cgctggctac
     2761 ggaaccggcg acgccatcgc cggccatgca accctcagcg cccgcgccca ttcaggtctc
     2821 tccggtccag ccgcctccgg ctcggcccgc tttgccgcaa tcccagcggg tcacatcgcc
     2881 cacggcagac aggctcgacc gccctatcgc caagccgttg ccgcaacccg cgaccacggc
     2941 cgagcctggc ccgctgccct ccatcgacgc catcaatccc gagcgtttcg gcgcgaggcc
     3001 ggc
//
LOCUS       DASYCS010000364         2662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4741057,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000364 DASYCS010000000
VERSION     DASYCS010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4741057"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="VGM46_11390"
     CDS             <1..801
                     /locus_tag="VGM46_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006205947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEY4193238.1"
                     /translation="GHRKIRQARLIATEACRAAENGVEFIERVSREAGLKLEIIDRQT
                     EARLAVSGCGSLIERDTQGVVLFDIGGGSSEIALIDLSGRRAPRLANNIVSWTSLPVG
                     VVSLAERFGGRAVSRETFAAMVEDVAGHLAAFEGRDRLDHIVNGSKFHLLGTSGTVTT
                     LAGVHLDLERYDRRRVDGLWMDRTSVDAMVDRLVGWDFQQRVANPCIGADRADLVLAG
                     CAILEAIRAVWPAERLRVADRGLREGILSELMADDGAWRNSWRGGYRQ"
     gene            798..1514
                     /locus_tag="VGM46_11395"
     CDS             798..1514
                     /locus_tag="VGM46_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018427331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEY4193239.1"
                     /translation="MTKKPEKPTTAGMRVMKTRIKKKSGLKESSRRWLERHMNDPYVQ
                     RSKVDGYKSRAAYKLIEIDDRYHILKPGMKIIDLGAAPGGWCQVAAVRTSSTAEKPHV
                     VGIDYLGMDPVPGTVVLEMDFLDDAAPAKLIETLGGEPDVVLSDMAAPTTGHRRTDHI
                     RTEHLCEVAADFAISVLRPGGHFLSKTFQGGTEHEVLALLKKNFRSVHHVKPPASRDE
                     SVELYLLAKDFKGRTPPEAE"
     gene            complement(1540..>2662)
                     /locus_tag="VGM46_11400"
     CDS             complement(1540..>2662)
                     /locus_tag="VGM46_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012093177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4193240.1"
                     /translation="MAFVVSRFLQGMGGAMMTPVGRLVLVRSTPKSELVSAMSWLSIP
                     ALVGPLVGPPVGGFITTYFSWHWIFLINVPIGVIGIWLATRYLPKTPSAITPRLDIPG
                     FILSGVAASGLVFGLSVMGLAALPAYVGIGTTAIGLLSGVLYLLHARNAPNPLLALSL
                     FRNQVFRASVLGGGLFRIGIGAVPFLLPLMLQIGFGLTPFQSGMITFVSAIGAMGMKF
                     ATAWVFRMAGFRKVLFFGSLVSAASVGINGFFTPQTPYALILVVLLAGGFLRSMFFTG
                     INALTYSEISAEDTSKATPIAAVGQQLSIALGVAVAGGILETSTRFHGGSLTLADFHI
                     AFFIVAAISALAAIWFLRLPPDAGSAVSGHGVEAAKPQA"
BASE COUNT          550 a          815 c          885 g          412 t
ORIGIN      
        1 ggccatcgca agataaggca ggcgcggctg attgcgacgg aggcgtgccg ggcggcggaa
       61 aacggcgtcg aattcatcga gcgcgtttcg cgcgaagccg ggctgaaact ggagatcatc
      121 gaccggcaga cggaagcgcg gctggccgtt tcgggctgcg gctcgctgat cgagcgggat
      181 acgcagggcg tcgttttgtt cgacatcggc ggcgggtcgt ccgaaatcgc gctgatcgac
      241 ctgtcgggcc ggcgcgcgcc acggctggct aacaacatcg tgtcgtggac atcactgccg
      301 gttggcgtcg tctcgcttgc cgaacgcttc ggcggccgtg ctgtgagccg ggaaaccttc
      361 gctgccatgg tcgaagacgt cgccggacac cttgcggcct tcgaaggccg cgaccggctg
      421 gaccatatcg tcaatggctc gaagttccat ctgctcggca cttccggcac ggtaacgacg
      481 ctggccggcg tgcatctcga cctcgagcgt tacgaccgcc gccgcgtcga tgggctgtgg
      541 atggaccgca ccagcgtcga tgcgatggtg gacaggctcg tcggctggga tttccagcag
      601 cgcgtcgcca atccctgcat cggcgcggat cgcgccgacc tggtgcttgc cggctgcgcc
      661 atcctggaag ccatccgcgc ggtgtggccg gccgagcggc tgcgcgttgc cgaccggggc
      721 cttcgcgaag gcatattgag cgagctgatg gccgatgacg gcgcatggcg caacagctgg
      781 cgaggcggat atcggcaatg accaagaaac ctgaaaagcc gaccacggcc ggcatgcgtg
      841 tcatgaagac gcgcatcaag aagaaaagcg gcctgaagga atcgtcgcgg cgctggctgg
      901 agcgtcacat gaacgatcca tatgtccagc gttccaaggt cgatggctat aagtcgcgcg
      961 ccgcctataa gctcatcgag atcgacgacc gctaccatat tctcaagccc ggcatgaaga
     1021 tcatcgatct cggggcggcg ccgggcggct ggtgccaggt ggccgcggtg cgcaccagtt
     1081 cgacggcgga aaagccgcac gtcgtgggca tcgactatct cggcatggac cctgtgccgg
     1141 ggaccgtggt cctcgaaatg gatttcctcg acgatgcagc ccccgcaaag ctcatcgaga
     1201 cgctgggcgg cgaacccgac gtggtgctgt cggacatggc cgcgccgacg accggacacc
     1261 ggcgcaccga ccatatccgc acggaacatc tatgcgaggt ggccgccgat tttgccattt
     1321 cggtgcttcg acccggcggc catttcctgt cgaaaacgtt ccagggcgga actgaacatg
     1381 aagtgttggc gcttttgaag aagaatttcc gctcggtcca tcacgtcaag ccgccggcgt
     1441 cgcgtgacga atcagtggag ctttatctgc tggccaagga ctttaagggt cgcaccccgc
     1501 ccgaggccga atagcggcgt cctggtcgaa ggccgcgctc tacgcctgcg gtttggccgc
     1561 ctccacgccg tggcccgata cggcgctgcc ggcgtcgggc ggcagacgca ggaaccagat
     1621 cgccgccagt gcggatattg ccgccacgat gaagaacgct atgtggaaat ccgccagcgt
     1681 cagcgagccg ccgtgaaagc gcgtcgaggt ttccagaatg ccgccggcga ccgccacgcc
     1741 gagtgcgatg gaaagctgct ggccgaccgc ggcgatgggc gtggccttgc tggtgtcctc
     1801 cgccgaaatc tcggaatagg tcagcgcgtt gatgcccgtg aagaacatgg agcgcaggaa
     1861 gccgccggcc aataatacga cgaggatcag cgcgtatggc gtctgcggcg taaaaaagcc
     1921 gtttatgccg accgaggccg ccgaaaccag cgagccgaaa aacagcacct ttcgaaagcc
     1981 ggccatccgg aacacccagg cggtggcgaa tttcattccc atcgcaccga tcgccgagac
     2041 gaaggtgatc atgccggact ggaagggcgt cagcccaaag ccgatctgta gcatcagcgg
     2101 cagaaggaac ggcaccgcac cgatgccgat gcgaaacagg ccgccgccca gcacggaagc
     2161 gcggaaaacc tggttgcgga aaagcgatag cgcaagcaaa gggttcggcg cgttgcgcgc
     2221 atggagcagg taaagcacgc cggacaacag cccaatggcc gtggtgccga tgccgacata
     2281 ggcgggcaag gctgccaatc ccatcacgga gaggccgaaa accaaacccg aggcggccac
     2341 gccgcttagg atgaaacctg gaatgtcgag gcgcggggtg atcgccgaag gtgttttcgg
     2401 caggtacctg gtggcaagcc agatgccaat gacgcctatc ggcacattga tgaggaaaat
     2461 ccagtgccag gaaaaatagg tggtgatgaa gccgccgacc ggcggcccga ccagcgggcc
     2521 taccaaagcc ggaatgctga gccacgacat ggccgaaaca agctcgctct tgggcgtgga
     2581 acgcacaagc accaagcgcc ccaccggcgt catcatcgcg ccgcccatgc cctggagaaa
     2641 gcgggaaacg acgaaggcca tc
//
LOCUS       DASYCS010000365         1871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1842434,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000365 DASYCS010000000
VERSION     DASYCS010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1842434"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="VGM46_11405"
     CDS             <1..263
                     /locus_tag="VGM46_11405"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY4193241.1"
                     /translation="NRHGVHVPDDVSVVGFDDLEMSEHFIPPLTTIRQPRAAIGKAAA
                     TLLLAVMESERKGGSAMGNESRILPVELIVRGSTAALTPAQA"
     gene            complement(364..1689)
                     /gene="xylA"
                     /locus_tag="VGM46_11410"
     CDS             complement(364..1689)
                     /gene="xylA"
                     /locus_tag="VGM46_11410"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HEY4193242.1"
                     /translation="MNSGFFGDIKPARYEGPHSTNPLAYRHYNKDEVVAGKRMEDHLR
                     FAVAYWHSFGWPGGDPFGGQTFDRPWFPKAGGADTMELARLKADIAFEMFAALDAPYF
                     CFHDADVRPEGRDFAESAARLDEIADYFAGKMAKTGTKLLWGTANLFSNRRFMAGAAT
                     NPDPDVFAYSAATVKACMDVTKRLNGENYVLWGGREGYETLLNTDLKREQEQAGRFLS
                     LVVDYKHKTGFKGAILIEPKPQEPTKHQYDYDVATVYGFLKRFGLENEVKVNIEQGHA
                     ILAGHSFEHELALAGALGIFGSIDMNRNDYQSGWDTDQFPNNVPEMAIAYYQVLQAGG
                     FTTGGTNFDAKLRRQSLDPQDLLIGHIGAMDCCARGLKAAAKMVEDKALSAPLAERYA
                     GWNSDEAKAMLSGKRTLEEIAKRVVSEKIEPQPRSGRQEYLENVVNRYV"
BASE COUNT          333 a          609 c          562 g          367 t
ORIGIN      
        1 tgaaccgcca cggcgtccat gtgcccgacg atgtctcggt ggtcggcttc gacgacctcg
       61 aaatgtcgga acatttcatc ccgccgctga cgaccatccg ccagccgcgc gcagccatcg
      121 gcaaggcggc ggccaccctg ctgctggcgg tgatggagag cgagcgcaag ggcggcagcg
      181 ccatgggcaa cgaaagccgc atcctgccgg tcgagctgat cgtgcgcggc agcaccgccg
      241 ccttgacgcc cgcgcaagcg tgacccgcca tagcccgaca cttcacagcc tgatacggcc
      301 tgccatcgca taggacttcc tcctcccttt ccgccgcgcc cctccgaacg cggcatcgcc
      361 tcgtcagacg tagcggttga cgacgttctc cagatattcc tgcctgcccg acctcggctg
      421 cggctcgatc ttttccgaaa cgacgcgctt tgcgatctct tccagtgtgc gcttgcctga
      481 aagcatcgcc ttggcctcgt ccgaattcca gccggcataa cgctcggcca gcggagccga
      541 cagcgccttg tcctcgacca tcttggccgc cgccttcagg ccgcgcgcgc agcaatccat
      601 cgcgccgata tggccgatca gcaaatcctg cggatcgagc gactggcggc gcagcttggc
      661 gtcgaaattg gtgccgccgg tggtgaagcc gcccgcttgc agcacctgat aataggcgat
      721 cgccatttcc ggcacgttgt tgggaaactg gtcggtgtcc cagcccgact ggtaatcgtt
      781 gcggttcatg tcgatggaac cgaaaatgcc gagcgcgccg gccagcgcca actcatgctc
      841 gaaggaatgg ccggcgagaa tggcgtggcc ctgctcgata ttgaccttga cctcgttttc
      901 cagcccgaac cgcttcagga agccatagac ggtggcgacg tcatagtcgt actggtgctt
      961 ggtcggctcc tgcggcttcg gctcgatcag gatcgcgccc ttgaaaccgg tcttgtgctt
     1021 gtaatccacc accagcgaca ggaagcggcc cgcctgctcc tgctcccgct tcaaatcggt
     1081 gttgagcagc gtctcatagc cctcgcggcc gccccacagc acgtagttct cgccgttcag
     1141 gcgcttggtc acgtccatgc aggccttcac tgtcgccgcc gaataggcaa acacgtccgg
     1201 gtcgggattg gtcgccgcgc ccgccatgaa acggcgattg gaaaacagat tggcggtgcc
     1261 ccacaacagc ttggtgccgg tcttggccat cttgccggcg aaatagtcgg cgatctcgtc
     1321 gaggcgcgcc gcgctttcgg cgaagtcacg gccttccggc cgcacatcgg cgtcgtggaa
     1381 gcagaaataa ggggcgtcga gcgccgcgaa catctcgaag gcgatatcgg ccttcaaacg
     1441 ggccagctcc atcgtatctg cgccgccggc cttgggaaac catggccggt cgaaggtctg
     1501 gccaccgaac gggtcgccgc ccggccagcc gaaggagtgc cagtaggcga cggcgaagcg
     1561 cagatggtct tccatgcgct tgcccgcgac gacctcgtcc ttgttgtaat ggcgataggc
     1621 gagcggattg gtgctgtggg ggccttcata tcgggcgggc ttgatgtcgc cgaaaaatcc
     1681 gctgttcatg gcagtaaact tcctttttcc tcaatgctgc gaaatcaacc atggaaggcc
     1741 gtttcgttca gtttagatat acgtacctca agactagcac gatttgaccc gtcagggcag
     1801 gttgtcgcga agataaattt cgatgcggat gctttcctgt ccgtcgacga tcctggtgcc
     1861 gtcgatgcgc g
//
LOCUS       DASYCS010000366         5000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3147295,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000366 DASYCS010000000
VERSION     DASYCS010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 5000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5000
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3147295"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="VGM46_11415"
     CDS             complement(<1..624)
                     /locus_tag="VGM46_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018427974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY4193243.1"
                     /translation="MRRTIAWLAAAISLLAFVPQQASSEPKYAIAMQGEPALPPDYSH
                     FTYVNPDAPKGGAVTYCVVGTFDNLNPFIIKSLRTTARGMMDTIYGNLVFEPLMQRNA
                     DEAFGLYGLLADSADMSDDRKSIEFHLNPQAKWSDGEAVTPEDVLFTYDVFTEKGRPP
                     YSDRMKKIAKLEKTGEHSVRFTFNDQADREFPLIIALTPILPKHAFSK"
     gene            866..1492
                     /locus_tag="VGM46_11420"
     CDS             866..1492
                     /locus_tag="VGM46_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009755856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HEY4193244.1"
                     /translation="MTSLSSNAYRLSIMAAGVVGFLGAGLFASVAQEQQIPQGWFKAC
                     TKQEDVDICNVQNITTASNGQLITGISMIELKGKVNRKVFQVTVPTGRLVPPGIGLQV
                     DNGKAQKLDYVICFPDRCVAEVPLSDGLVASFKKGSSLTLTSVNFQNQPNPIKVALTG
                     FSGAYDGPPMKQSDIEDRQKKLQDFVAKNNEDFAKKLKDEQDKAKANK"
     gene            complement(1714..2196)
                     /gene="rpsI"
                     /locus_tag="VGM46_11425"
     CDS             complement(1714..2196)
                     /gene="rpsI"
                     /locus_tag="VGM46_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEY4193245.1"
                     /translation="MAELSSLAELGAATGNTNTQAAAPVHVQKLDKLGRAYATGKRKN
                     AIARVWVKPGSGKIVVNGKEFAAYFARPVLQMILNQPIIASNRAGQYDIIATVIGGGL
                     SGQAGAVRHGISKALTYYEPTLRAVLKKGGFLTRDSRVVERKKYGKAKARRSFQFSKR
                     "
     gene            complement(2198..2662)
                     /gene="rplM"
                     /locus_tag="VGM46_11430"
     CDS             complement(2198..2662)
                     /gene="rplM"
                     /locus_tag="VGM46_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006195473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEY4193246.1"
                     /translation="MTTFSQKPADVVKKWVLIDAEGLVVGRVATIIANILRGKNKPTF
                     TPHVDDGDNVVVINADKVVFTGKKFTDKVYYWHTGHPGGIKERTARQLLEGRFPERVL
                     EKAVERMVPRGPLGRRQMKSLRVYAGTEHPHVAQQPTVLDVAALNSKNKKVS"
     gene            complement(2908..3360)
                     /locus_tag="VGM46_11435"
     CDS             complement(2908..3360)
                     /locus_tag="VGM46_11435"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEY4193247.1"
                     /translation="MPVQANIQPVLSAAQVNELMNFAYPQLNQGGRFYETIEVFPGGC
                     TVRLNADDRHLRPGGTVSGPSLFTLADIGGYACVLSHAGPDALSVTVSLTINFMRKAQ
                     AGPIDGHCRILKLGKNLMVFDIDLVTGPERHTVANATGTYSIPPKREK"
     gene            3504..4334
                     /locus_tag="VGM46_11440"
     CDS             3504..4334
                     /locus_tag="VGM46_11440"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010915640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEY4193248.1"
                     /translation="MAEIVALKTAPQGLVPGPVTAVLEKGVLRLTLASPPANALSLAV
                     MAALTAELDRARNDKAVRVIVLAASGKVFCAGHDLKELTAHRADADRGRAFFEHTFTA
                     CSTLMQAIVNNPKPVIGEVDGLATAAGLQLVASCDLAIASHEAKFCTPGVNIGLFCST
                     PMVALSRNATRKQAMEMLLTGETIDAATAREFGLVNRVVPREYLNQTVTKYAQTIASK
                     SPLTVKIGKEAFYAQAEMGLGDAYDYTGRVMVENMLARDAEEGIGAFIGKRAPEWKGE
                     "
     gene            4337..4756
                     /locus_tag="VGM46_11445"
     CDS             4337..4756
                     /locus_tag="VGM46_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006201857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY4193249.1"
                     /translation="MEPRISIITIAVEDLDRAAAFYEAMGLTRHRIRDGVAFFQMGGA
                     ILGLFPRTDAEKDAGIAFGDGPSRVYLAYNTRCEAEVGEVLAQAEKAGGRIVKPAGRA
                     FWGGWYGYFADTEGHVWEVAHNPDFPIDAQGAIALPA"
     gene            4753..>5000
                     /locus_tag="VGM46_11450"
     CDS             4753..>5000
                     /locus_tag="VGM46_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEY4193250.1"
                     /translation="MNHDTYDNAYIAGILNPVKTIAMVGASANEVRPSFFVLKYLLAK
                     GFSVFPVNPGHAGKEILGRMTYAKLADIPEPIDMIDIFR"
BASE COUNT          972 a         1452 c         1591 g          985 t
ORIGIN      
        1 cttgctgaag gcgtgcttgg gcaggatagg cgtcagcgcg atgatgagcg ggaattcacg
       61 gtcggcctgg tcgttgaagg tgaagcgcac gctgtgctcg ccggtctttt ccagcttggc
      121 gattttcttc atgcggtcgg agtagggcgg ccggcccttt tcggtgaaca cgtcataggt
      181 gaacagcacg tcctcgggcg tcaccgcttc gccatccgac cattttgctt gcgggttcag
      241 gtggaattcg atcgacttgc ggtcgtcact catgtcggcg ctgtccgcca gaagcccata
      301 gaggccgaac gcttcgtcgg cattgcgctg catcagcggc tcgaagacca gattgccgta
      361 gatcgtgtcc atcatgccgc gtgcggtggt gcgcaggctc ttgatgatga acggattgag
      421 attgtcgaag gtgccgacca cgcaataggt gaccgcgccg cctttcggcg catccgggtt
      481 gacgtaggtg aaatggctgt aatcgggcgg cagcgccggc tcgccctgca tggcgatggc
      541 gtatttaggc tccgaagaag cctgctgcgg cacgaaggcg agaagcgaaa tggcggcggc
      601 cagccaggca atggtgcggc gcatgaccgt ctcccgtggg gctcccttac ggaggctagt
      661 tgattcgaac tgcacgatac catggggcgg cggtacgttc ggcaggcgtg gacgcaagaa
      721 atgcgtgtgc cgggctggat tcatcccgcc agggagtgta acaccgccgc cgaagtcatt
      781 ctgttgcctc atttcggcaa caggtagcgg ggctacgcgg cgcgaaaatg gcgtcgcctg
      841 gatcatttga gaggaaatgc accacatgac gagcctcagc agcaacgcat accgcctttc
      901 gatcatggcc gcaggcgtgg tcggctttct cggtgcgggg ctttttgcat ccgtggcgca
      961 ggaacagcag atcccgcagg gctggttcaa ggcctgcacc aagcaggaag acgtcgacat
     1021 ctgcaacgtg cagaacatca ccaccgccag caacggccag ctcatcaccg gcatcagcat
     1081 gatcgagctg aagggcaagg tgaaccgcaa ggtcttccag gtaacggtgc cgaccggccg
     1141 cctcgttcct cccggcatcg gcctccaggt tgataacggc aaggcgcaga agctcgacta
     1201 cgtgatctgc ttcccggacc gctgcgtggc cgaagtcccg ctgtccgacg gccttgtcgc
     1261 ctcgttcaag aaggggtcgt cgctgacgct tacctcggtc aatttccaga atcagccgaa
     1321 cccgatcaag gttgcgctga ccggcttcag cggcgcctat gacggcccgc ccatgaagca
     1381 gtcggacatc gaggatcgcc agaagaagct tcaggatttc gtcgccaaga acaacgagga
     1441 cttcgccaag aagctcaagg acgagcagga caaggccaag gccaacaagt aacgcacctt
     1501 tcgtcgggct tggtcccgga cgcaaaaagg ctccggaaat tccggagcct tttttgtttg
     1561 tgccgatgtg ccggcaagcc aaaagtggtt tgcccaaaag cggttgcggc caatgaaata
     1621 aagcccggca atcctggcca ataaaaaagg ccgcatcgtt gccgatgcag cctttgtata
     1681 aagaccccgg cacggatgcc gggggagaaa aaatcagcgc ttcgagaact ggaagctgcg
     1741 gcgggccttc gccttgccgt acttcttacg ctcgaccacg cggctgtcgc gggtcaggaa
     1801 gccgcccttc ttgagcacgg cgcgcagcgt cggctcgtaa taggtcagcg ccttggaaat
     1861 gccgtggcgc accgcacctg cctggccgga aaggccgccg ccgatgacgg tggcgatgat
     1921 gtcgtactgg ccggcgcggt tggaggcgat gatcggctgg ttgaggatca tctggagcac
     1981 ggggcgcgcg aaataggccg cgaattcctt gccgttgacg acgatcttgc ccgagccggg
     2041 cttcacccag acgcgcgcga tggcgttctt gcgcttgccg gtggcatagg cgcggcccag
     2101 cttgtcgagc ttctggacgt ggacgggcgc ggcggcttgc gtattggtgt tgccggtcgc
     2161 ggcgcccagt tctgcgagcg aggaaagctc agccatatca ggaaaccttc ttgttcttgg
     2221 agttcagcgc ggccacgtcg agtaccgtgg gctgctgggc gacgtgcgga tgttccgtgc
     2281 ctgcatagac gcgaaggctc ttcatctggc gacggccgag cggaccgcgc ggaaccatgc
     2341 gctcgacggc cttttcaagg acgcgctcgg ggaaacgtcc ctcaagcagc tggcgcgcgg
     2401 tgcgctcctt gatgccgccg ggatggccgg tgtgccagta atagaccttg tcggtgaatt
     2461 tcttgccggt gaacacgacc ttgtcggcat tgatgacaac gacattgtcg ccgtcgtcga
     2521 cgtggggggt aaaggtcggc ttgttcttgc cacgcaggat gttggcgatg atggtggcga
     2581 cacggccgac gacgagaccc tcggcgtcga tcagcaccca cttcttcacc acgtccgcag
     2641 gcttctgcga aaaggttgtc atggatttcg ttctttcagg ttccagacct tgcccacccg
     2701 gaaatcggag aggaaggcgt ttcttgttgc ttggattggc aggaagccag gctccggcca
     2761 ataataaaac ggcggccttt gcgggccgct gcttcgttgg ggtttatagg cgagggcgcc
     2821 gcgcgcgtca atcgaccggg aacggcaatc attcgaagaa acatgttcaa aaacagacag
     2881 atagaagtga ggtattattt taccgcatca tttttcgcgc ttcggcggaa tggaataggt
     2941 gccggtggcg tttgcgaccg tgtggcgttc cggcccggtg acaaggtcga tatcgaacac
     3001 catcaggttt ttgccgagct tgaggatgcg gcaatgcccg tcgatcggcc cggcctgcgc
     3061 cttgcgcatg aaattgatgg tgaggctgac ggtaaccgac agcgcatccg gcccggcatg
     3121 cgagagcacg caggcatagc cgccaatatc ggccagcgtg aacagcgacg gtcccgaaac
     3181 ggtgccgccg gggcgcagat gccggtcgtc ggcgttgagc cgcaccgtgc agccgccggg
     3241 aaacacctcg atcgtctcgt agaaccggcc accttgatta agctgcggat aggcgaagtt
     3301 catcagctcg ttgacttgcg ctgccgaaag aaccggctgg atattggctt ggacgggcat
     3361 tgctgcttcc tcattggcgc tgcctttatg gccgcaccgc cgcctctgat gcaacatgtt
     3421 gaagagagct gtttgaccag atgggatatc ctattaagtt tgcatgatgg catcagccaa
     3481 cgcctgaaga gtggagagaa gacatggccg agatcgttgc cctcaagacc gcgccacagg
     3541 gcttggttcc tggtccggta acggccgtgc tggagaaggg cgttctgcgg ctgacgcttg
     3601 ccagcccgcc ggccaatgcg ctttcgcttg ccgtgatggc tgcgctgacc gccgaactcg
     3661 accgcgcgcg aaatgacaag gccgtgcgcg tcattgtgct ggctgcctcc ggcaaggtct
     3721 tttgcgccgg ccacgatttg aaggaactca ccgcccatcg cgccgatgcc gaccggggcc
     3781 gggcgttctt cgaacacacc ttcaccgcct gttcgacgct gatgcaggca atcgtgaaca
     3841 atccgaagcc ggtgatcggc gaggtcgatg ggctggccac cgccgccggc ctgcaactgg
     3901 tcgcgagctg cgacctggcc atcgcctcgc atgaggcgaa attctgcacg ccgggcgtca
     3961 atatcggcct gttctgctcg acgccgatgg tggcgctgtc gcgcaatgcc acgcgcaagc
     4021 aggcgatgga aatgctcctg acgggcgaga ccatcgacgc cgcgacagcc agggaattcg
     4081 gcctcgtcaa ccgtgtggtg ccgcgcgaat acttgaatca gactgtgacc aaatatgcgc
     4141 aaaccattgc ttccaaatcg cctttgacgg tgaagatcgg caaggaggcc ttttatgcgc
     4201 aggccgaaat ggggctgggt gacgcttacg attatacggg ccgcgtcatg gtcgaaaaca
     4261 tgctggcgcg cgacgccgag gaaggcatcg gcgctttcat cggcaagcgc gcgccggaat
     4321 ggaaggggga atagaaatgg aaccgcgcat ctccatcatc accatcgccg tcgaggatct
     4381 cgaccgcgcg gctgcgtttt atgaggcgat gggcctgacc cgccaccgca tccgcgatgg
     4441 cgtcgccttc ttccagatgg gtggcgcgat tttgggattg ttcccccgca ccgatgccga
     4501 aaaagacgcc ggcattgctt ttggcgacgg accctcgcgc gtctatcttg cctacaatac
     4561 ccgttgcgag gcagaggtgg gagaagtgtt ggcgcaggct gaaaaggccg gcgggcgcat
     4621 cgtcaagccg gcaggccggg ctttctgggg cggctggtac ggctattttg ccgatacgga
     4681 aggccatgtc tgggaagtcg cgcacaatcc tgactttccg atcgatgccc aaggcgctat
     4741 agccttgccc gcatgaacca cgacacctat gacaacgcct atatcgcggg cattctcaac
     4801 ccggtgaaga ccatcgccat ggtcggcgcc tcggcaaatg aggtgcggcc aagcttcttc
     4861 gtgctgaaat atctgctcgc caagggtttt tcggtctttc cggtcaatcc cggccatgcc
     4921 ggcaaggaaa ttcttggccg catgacctat gccaaactcg ccgacattcc cgaacccatc
     4981 gacatgatcg atattttccg
//
LOCUS       DASYCS010000367         6304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4015002,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000367 DASYCS010000000
VERSION     DASYCS010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 6304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6304
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4015002"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..352
                     /locus_tag="VGM46_11455"
     CDS             80..352
                     /locus_tag="VGM46_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193251.1"
                     /translation="MSEHAIEFLQEWICEKVQAPAHPAMVEKHAATLARECAAKAAEA
                     GIPLEDIQEEVGDIQDLIAAKLEDAAEAENTSGDGMDADNENKPAK"
     gene            complement(404..2083)
                     /gene="xseA"
                     /locus_tag="VGM46_11460"
     CDS             complement(404..2083)
                     /gene="xseA"
                     /locus_tag="VGM46_11460"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEY4193252.1"
                     /translation="MNETAPESRTNATEYTVSEISGALKRTVEDTFGNVRVRGEISGY
                     RGPHSSGHAYFTLKDDRSRLEAVVWKGTMGRLKFRPEEGMEVIATGKLTTYPGSSKYQ
                     IVIDNLEPAGAGALMALLEERKRRLQAEGLFDPARKQLLPFMPRVIGVVTSPTGAVIR
                     DIVHRIKDRFPLHVIIWPVRVQGETSGAEVAAAVQGFNALAADGAIPTPDLLIVARGG
                     GSLEDLWGFNDEALARAVAASGIPVISAVGHETDWTLIDLAADVRAPTPTGAAEIAVP
                     VKAELEATLASLGARLRSSVSRGVDRKRQAARAAARALPSPDQLLALPRRRFDDATSR
                     LDRALVIGVERKRARLGALRLSPATLSRRIREWRKVADRDLMRAQVALRAVAREKRAE
                     LRKASDQLPKIASAARALKQRRFEAAAARLSFQPLQRATQAARSRLDGAARRHDHALD
                     ARLERLRGRLSQADRLLSTLKLSDQAILERGYALVLDAAGALVKRAVDIKPGEMLELR
                     FADGAAAATAGGAAGDGQAGQKPQIDAKTQARATPKPAAKAREPGNQGSLF"
     gene            complement(2133..3089)
                     /locus_tag="VGM46_11465"
     CDS             complement(2133..3089)
                     /locus_tag="VGM46_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008877388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY4193253.1"
                     /translation="MKAPLDLDQLQTFISISDTGSFTRAADEVNRTQSAVSMQMRRLE
                     ERIGKPLFEKDGRVNRLTEDGERLLSYARRLIFLNRETLAAFDDQRLEGTIRIGTPDD
                     YADRFLPEIMARFARSNPRVELTVVCEPTPGLVEHIRRGNLDIALVTHNDTRGQAEVV
                     RREPLMWVSSANHATHEEAVLPMAFGRPNCIWRLAACDVLCDMKREYRILFTSFSATV
                     IAAAVLSGLAISVLPECALRTGMRVLGEADGFGALPDCRIAILRGQTSRPELVDALAR
                     HISESLDNLSVPMGDEAAGFDFAAPALSKTKRAKPSQLVPGW"
     gene            3107..3571
                     /locus_tag="VGM46_11470"
     CDS             3107..3571
                     /locus_tag="VGM46_11470"
                     /inference="COORDINATES: protein motif:HMM:NF018294.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HEY4193254.1"
                     /translation="MLMPEIKNIRWINQLRHVSFRPSRKRHEKKHQAAAFGGANGWSA
                     SMMGELVRSMKEMTMSTCELASETPRITTRPAVAARAVNTIAAFLRAWKNRRAFYRLG
                     EMSNAELADIGLTRADLHVAIDIPFGSDPTTRLRALASDRAASAERMARMVG"
     gene            complement(3808..4800)
                     /locus_tag="VGM46_11475"
     CDS             complement(3808..4800)
                     /locus_tag="VGM46_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010914410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HEY4193255.1"
                     /translation="MLSLFDMLSNAQDGQGMDVLAKQFNLSQQQAQLAVEALLPAFSQ
                     GLKRNTADPAGLGSFLSSMASGQHAAFFDNADSAFSAHGVSQGNDVLGQLFGSKDLSR
                     AVASQAAQTTGLSQQLLQQMLPVIASMVMGGLFKQSTNQMPAAKAAAGGNNPLAEIIE
                     QMMKQGGLGGMLGGQSAPQQAPQQQAPQSQNPFDQMGNNPLGKVLQDMFGGGAVRQQT
                     QNPAGADPGANPGANPWGKILEDMLGGGQQSGRQQSGGQQGGAPQGGGRQAGQRQAQQ
                     PEVPQPQAPQPETTSQNPYGDIFGKMFETGKQQQDDYQKGMESIFDQFKKGMGR"
     gene            complement(4885..6258)
                     /locus_tag="VGM46_11480"
     CDS             complement(4885..6258)
                     /locus_tag="VGM46_11480"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011982724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEY4193256.1"
                     /translation="MARDTTDFRPIEGVDELVEHLAEGNKPREKWRIGTEHEKFPFYV
                     DGNAPVPYGGERGIRAILEGMQQKLGWDPIVDEGRIIGLVEPTGQGAISLEPGGQFEL
                     SGAPLETIHQTCREGNAHLSQVREIAEPLGIRFLGLGGSPKWSLAETPVMPKSRYEIM
                     TRYMPKVGTKGLDMMYRTCTIQVNLDFESEADMRRKMQVSLKLQPLSTALFANSPFTD
                     GKPNNFLSWRGDIWRDTDNKRSGLLEFCFSPEFGFADYVEWALDAPMYFIIRDGRYHD
                     MTHMTFRQFMAGAARKEITDGVPTIGDWANHLSTLFPDVRLKRFLEMRGADGGPWRRI
                     CALPAFWVGLLYDEAALDAAEAMTANWSYGEVLAMRDAVPEQGISAPFRNTTLRDVAR
                     DVLAISRDGLKRRGKLNRDGYDETSFLSTLDEVVARGTTSAEEMLGAYHTRWGNSIEP
                     VFLEYAY"
BASE COUNT         1067 a         2043 c         2022 g         1172 t
ORIGIN      
        1 tattcgggcg aaatgattcg ggcatctttt ggcggggtca tgcgttgaat atgacgaatc
       61 ggcttgaggt ttttcgggca tgagcgagca cgcaatcgaa ttcttgcagg aatggatctg
      121 cgaaaaggtt caggctccgg cgcacccggc gatggtcgaa aagcacgccg caacgctggc
      181 cagggaatgc gcggccaagg ccgccgaggc gggcatccct ctggaagata tccaggagga
      241 ggtcggcgac attcaagacc tcatcgccgc caagcttgag gatgcggccg aagccgaaaa
      301 tacttccggt gacggcatgg atgccgacaa cgaaaacaag cctgctaaat aagttcagcc
      361 cattggcctg ccggcgcggc atggccggct gctcccgctt tttttaaaac agtgaaccct
      421 ggttgccggg ttcccgcgcc ttggcggcgg gctttggcgt agcgcgcgcc tgtgtcttgg
      481 cgtcgatctg cggcttttgg ccggcttgcc catcgcccgc tgcgcccccg gcggtggccg
      541 cagccgctcc atcggcaaag cgcagttcca gcatctcgcc aggctttatg tcgactgcgc
      601 gcttcaccag cgcgccggcc gcgtccagca ccagcgcgta gccgcgttcg aggatcgcct
      661 ggtcggaaag cttcagcgtc gataggagcc ggtccgcctg gctgagcctg ccgcgcaggc
      721 gttccagccg ggcatccagc gcatggtcat gccggcgcgc cgcgccgtcc agccgcgacc
      781 gcgcggcttg cgtggcccgc tgcaatggct ggaaggagag ccgcgcggcg gccgcctcga
      841 accgcctttg cttcaacgcc cgtgcggcgc tggcgatttt cggcaattgg tcggatgcct
      901 tgcgcaactc cgcccgcttt tcgcgcgcga cggcgcgcag ggccacctgc gcccgcatca
      961 gatcccggtc ggcgaccttg cgccattcgc gaatgcgccg cgacagcgtc gctggtgaaa
     1021 gcctgagcgc gccaagccgc gcccgcttgc gctcgacgcc gatgaccagc gcccggtcga
     1081 gccggctggt cgcgtcgtcg aaacggcggc gcggcagggc cagcaactgg tcgggcgagg
     1141 gcagcgcccg cgcggcggcg cgcgccgcct ggcgcttgcg gtcgacgccg cgcgaaacgc
     1201 tgctgcgcag gcgcgcgcca aggctggcca gcgtcgcttc aagttcggcc ttcaccggca
     1261 ccgctatttc ggcggcgccc gttggcgtcg gcgcgcggac atctgcggcg aggtcgatca
     1321 atgtccagtc ggtttcatgg ccgaccgccg agatgaccgg aatgccggac gccgccacgg
     1381 cgcgcgccag cgcctcgtca ttgaaccccc acaaatcttc caggctgccg ccgccgcgcg
     1441 ccacgatcag aaggtcgggc gtcgggatag cgccatccgc cgccagcgcg ttgaagccct
     1501 gcacggccgc cgccacctcc gcgccggacg tctcgccctg cacccgcacc ggccagatga
     1561 tgacatgcag cggaaagcgg tccttgatgc ggtggacgat gtcgcggatg acggcgcccg
     1621 tcggcgaggt gacgacgccg atgacgcgcg gcatgaacgg caacagctgc ttgcgcgccg
     1681 ggtcgaacag cccttccgcc tgtagccgcc gcttgcgttc ctccagcagc gccatcagcg
     1741 cgccggcccc ggccggttcg agattgtcga tgacgatctg gtatttcgac gaaccgggat
     1801 aggtggtcag cttgccggtg gcgatcacct ccatgccctc ttcgggccgg aatttcagcc
     1861 gccccatcgt gcccttccac accacggctt ccagccgcga gcggtcgtcc ttcagcgtga
     1921 aataggcatg cccggaggag tgcggcccgc gatagccgga aatctcgccg cgcacgcgca
     1981 cattgccgaa cgtatcctcc acggtgcgct tcagcgcgcc ggaaatctcg ctcaccgtat
     2041 attcggtggc gttggtgcgt gattcggggg cggtctcgtt catggcgcga ttgagcggct
     2101 aatggtgctt cgggtcaagg ctgcggccac ctctaccagc ccggcacaag ctggctcggc
     2161 tttgcccgct tcgtcttcga cagggccgga gcggcgaaat cgaaaccggc ggcttcatcg
     2221 cccatcggca cggaaagatt gtccaggctt tccgaaatat ggcgcgccag cgcatcgacc
     2281 agttccgggc gcgaggtctg cccgcgcaga atggcgatgc ggcaatcggg aagcgcgcca
     2341 aagccgtccg cctcgcccaa aacgcgcatg ccggtgcgca aggcgcactc cggcagcacc
     2401 gaaatggcca gacccgaaag cacggcggcg gcgatgacgg tggccgaaaa actggtgaag
     2461 agaatgcgat attcccgctt catgtcgcag agtacgtcac aggcggccag ccgccagatg
     2521 cagttgggac gcccgaacgc catcggcaaa accgcctcct catgcgttgc atggttggcc
     2581 gagctgaccc acatcagcgg ttcgcgccgc accacttccg cctggccgcg cgtgtcgttg
     2641 tgggtcacca gcgcaatgtc cagattgccg cgccggatat gctcgaccag gccgggcgtc
     2701 ggctcgcaaa cgacggtcag ttcaacgcgc gggttggagc gggcaaagcg cgccatgatc
     2761 tcgggcagga agcggtcggc atagtcgtcg ggcgtgccga tgcggatcgt tccctccagc
     2821 cgctggtcgt cgaaggcggc caaggtctcg cggttaagga agatgagccg ccgcgcatag
     2881 gaaagcagcc tttcgccatc ctcggtcaac ctgttgacgc gcccgtcctt ctcgaacagc
     2941 ggcttgccga tccgctcctc cagccggcgc atctgcatgg aaaccgccga ctgcgtgcgg
     3001 ttcacctcat cagccgcgcg ggtgaagctg ccggtgtcgg aaatcgagat gaaagtctga
     3061 agctggtcga gatcgagcgg cgctttcatg atcgaaccca tcattgatgt tgatgccaga
     3121 gattaaaaac attcgttgga ttaatcaatt gcgccatgtc agtttccgac cgtcccgcaa
     3181 gaggcatgaa aagaagcatc aggcggccgc ttttggcggc gccaatggct ggtccgcgtc
     3241 catgatgggc gagcttgtcc gatcgatgaa ggaaatgacc atgagcacct gtgaattagc
     3301 ctccgaaacc ccgcgcatca ccacgcgtcc ggctgttgcg gcgcgtgcgg tcaatactat
     3361 cgctgctttc cttcgtgcgt ggaaaaaccg gcgcgctttt tatcgccttg gcgaaatgtc
     3421 gaatgccgaa ctcgccgata tcggcctgac gcgcgccgac ctgcacgtcg ccatcgacat
     3481 tcccttcggc agcgatccga cgacgaggct gcgcgcgctg gcaagcgacc gtgcggcgag
     3541 cgccgaacgc atggcccgca tggtcggctg accgttttgc aggctaccga ccctgccggt
     3601 ccccctccgg ccagcctgca tcctgcccgg tcccgtcagg ggccgggctt tttcgtggca
     3661 tgagccgagg cagttcccga ttgccggctc gatcggatgt gcctttagag catttccagc
     3721 aaaagtgtga accggttttg cgtccggagg tgctgtcaaa caaagagata gagcggtttt
     3781 gtcgattggg taaaaaccag aaccgctcta gcggcccatg cccttcttga actggtcgaa
     3841 gatcgattcc atgcccttct ggtaatcatc ctgctgctgc ttgccggtct cgaacatctt
     3901 gccgaaaata tcgccatagg gattttgcga ggtggtttca ggttgcgggg cctgcggttg
     3961 cgggacctcc ggctgctggg cctggcgctg cccggcctgc cggccgccgc cttgcggagc
     4021 gccaccctgt tgaccaccgc tttgctgcct cccgctttgc tggccaccgc ccagcatgtc
     4081 ttcgagaatc ttgccccaag ggttggcgcc cggattggcg cccggatcgg cccctgcggg
     4141 gttctgcgtc tgctgccgga cggcgccacc gccaaacatg tcctgcaaca ccttgccgag
     4201 cgggttgttg cccatctggt cgaacgggtt ttgcgattgc ggagcctgct gctgcggggc
     4261 ctgctgcggt gcggattggc cgcccagcat tccgcccagg ccaccctgct tcatcatctg
     4321 ctcgatgatt tcggccagtg gattgttgcc gccggcggcg gccttggcgg ccggcatctg
     4381 gttggtcgat tgcttgaaca ggccgcccat caccatcgag gcgatgaccg gcagcatctg
     4441 ttgcagcaat tgctggctaa ggccggtcgt ctgtgccgcc tgcgaggcga ccgcgcggga
     4501 aaggtctttg gagccgaaca actggccaag cacatcattg ccctggctta cgccatgcgc
     4561 ggaaaaggcg ctgtcggcat tgtcgaaaaa ggcggcgtgc tggccgctcg ccatggaaga
     4621 aaggaaggaa cccagcccgg cagggtccgc cgtgttgcgc ttcaatcctt gtgaaaaagc
     4681 cggcagtagc gcctcgaccg caagctgcgc ctgctgctgc gacaggttga actgtttggc
     4741 cagcacatcc atgccctggc cgtcctgcgc gtttgacagc atgtcgaaca aagaaagcat
     4801 cgtctgtttc tccatgggga caccgctccc tacggtcaga ctaatgcctt tctgcaacct
     4861 gttgaagtaa tttcaggcaa atattcagta ggcgtattcc aggaacaccg gttcgatgga
     4921 attgccccag cgcgtgtgat aggcgccgag catttcttcg gcacttgtcg tgccgcgcgc
     4981 caccacctcg tcgagcgtcg acaggaagct ggtttcgtca tagccgtcgc ggttcagctt
     5041 gccgcgccgc ttgaggccgt cgcgcgaaat ggcgagcacg tcgcgggcga cgtcccgcag
     5101 cgtcgtgttg cggaacggcg ccgagatgcc ctgttccggc acggcgtcgc gcatggccag
     5161 cacttccccg tagctccagt tggcggtcat cgcctcggcc gcgtctagcg ccgcctcgtc
     5221 gtaaagcagg ccgacccaga aggccggcaa cgcgcagatg cgccgccatg ggccgccatc
     5281 ggcgccgcgc atttcgagga agcgcttcag ccgcacatcg ggaaacagcg tcgacagatg
     5341 gttcgcccag tcgccgatcg tcggcacgcc atcggtgatt tccttgcgcg ccgcgccggc
     5401 catgaactgc cggaacgtca tatgcgtcat gtcgtgatag cggccgtcgc ggatgatgaa
     5461 atacatcggc gcatccagcg cccattcgac ataatcggca aagccgaatt cgggcgagaa
     5521 gcagaattcg agcaggcccg agcgcttatt gtcggtgtcg cgccagatgt cgccgcgcca
     5581 cgacaggaaa ttgttcggct tgccgtcggt aaacggcgaa ttggcgaaca gcgccgtcga
     5641 cagcggttgc agcttcagcg acacctgcat cttgcggcgc atgtcggctt cgctttcgaa
     5701 gtcgagatta acctggatgg tgcaggtccg gtacatcatg tcgaggccct tggtgccgac
     5761 cttgggcatg tagcgggtca tgatctcgta gcgcgatttg ggcatcaccg gcgtctcggc
     5821 cagcgaccat ttcgggctgc cgccgaggcc gaggaagcgg atgccgagcg gttcggcgat
     5881 ttcgcgcacc tgcgacaggt gggcgttgcc ttcgcggcag gtctggtgga tcgtctccag
     5941 cggcgcgccg gaaagttcga actggccgcc cggctccagg ctgatggcgc cctggccggt
     6001 cggttcgacc agcccgatga tacgcccttc atcgacgatc gggtcccagc ccagtttctg
     6061 ctgcatgcct tccaggatgg cgcggatgcc acgctcgccg ccatagggca cgggcgcgtt
     6121 gccatcgaca tagaagggaa acttctcgtg ctcggtgccg atgcgccatt tttcgcgcgg
     6181 cttgttgcct tccgccagat gttcgacaag ttcgtcgacg ccttcgatag gccggaaatc
     6241 ggtcgtgtcg cgtgccatga aagcatctcc tgagcggccg gaaggctgct tgcgctagat
     6301 ggcg
//
LOCUS       DASYCS010000368         5372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1410708,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000368 DASYCS010000000
VERSION     DASYCS010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 5372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5372
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1410708"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            6..950
                     /locus_tag="VGM46_11485"
     CDS             6..950
                     /locus_tag="VGM46_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010915065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEY4193257.1"
                     /translation="MHPALDSFSHLRAHLAVLLALLALALLAFAGFRTEAAVALLFAF
                     GAFSTTSSALSLPWLSPVQAGFVSGPPERAAYRLLQMNLRFDNRTPEKVLSLIGRTQP
                     DVITLDEVSVMWKAKFELLASAYPHRILCPYPDRPFGVAILSRRPFVAGTAPRCFTRG
                     ALAIATVDFGGAPVDIAAIHIGWPWPFEQGWQVGELTSHLDLLGETALMAGDCNAVPW
                     SATVRTVAKAGGLKLMPSVGPTWQYMKLPDFLRFAGLPIDQVFSKGAVLIHSASRLED
                     TGSDHLPVLVEFSLRPQPDQPDDEHATATASAAVSRRM"
     gene            complement(1000..2679)
                     /locus_tag="VGM46_11490"
     CDS             complement(1000..2679)
                     /locus_tag="VGM46_11490"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004685519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEY4193258.1"
                     /translation="MSDIERESMEFDVVIVGAGPAGLSAAIRLKQINPELSVVVLEKG
                     GEVGAHILSGAVVDPIGIDRLLPGWREDGDHPFKTPVTADHFLVLGPAGSIRLPNFMM
                     PPLMNNHGNYIVSLGNVCRWLAGKAEALGVEIYPGFAAVGLLYDDKGAVTGVVTGDMG
                     VEKDGSHGPSFAPGMALMGKYVLIGEGARGSLAKQLIAKYNLSEGRDPGKFGIGLKEL
                     WQIKPENHKPGLVQHSFGWPLDMKTGGGSFLYHLEDNMVAVGFVLHLNYKNPWLSPFE
                     EFQRFKTHPAIRGTFEGGKRLGYGARAITEGGWQSVPKLSFPGGVLVGCAAGLVNVPR
                     IKGSHNAVLSGMLAADHVAEAVAAGRAHDELSSYEAAWRGTAIGKDLKMVRNVKPLWS
                     RFGTIVGVAVGGLDMWLNTLLGFSPFGTLKHGKTDAASLEPASQHKKIVYPKPDGVLT
                     FDRLSSVFLSNTNHEENEPVHLLVADMELQKRSEHDVFGGPSSRYCPAGVYEWVDADG
                     NAASDPSAKDVRFVINAQNCVHCKTCDIKDPNQNITWVPPQGGEGPVYQNM"
     gene            2956..3507
                     /locus_tag="VGM46_11495"
     CDS             2956..3507
                     /locus_tag="VGM46_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193259.1"
                     /translation="MLPFPANGQQMQTWPAGPYSFSDELGGFRITAVSGRGTHDDPVV
                     IQEELNSATPVTLTVRTTRPIRPFASSGDYANGILYMRMRVLNNSGQAWIEFEFELQE
                     ILGQPSVFGDGLSFDQRNKNPTNIVSSNFAGFDRDFEPYDRLLFKKGAIDPLKLASFD
                     FLITDYTPRWTFYLVQDPRIPSS"
     gene            3545..4432
                     /locus_tag="VGM46_11500"
     CDS             3545..4432
                     /locus_tag="VGM46_11500"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEY4193260.1"
                     /translation="MNAASPNNRPDLRELLAFYAEAGVDEPLTDEPVDRFAQVPAPRS
                     EQGRPSVAAAVERAGEADGRPELSGENRTNEPPRAAQRPATPKAAIPDEAQAGLAREL
                     AASATTLAELREAMASFEGCNLKLTAKNMVFADGDPQARLMLVGEAPGRDEDIEGRPF
                     VGRSGQLLDRMLAAIGIDRMSVYIANVIPWRPPGNRTPTPQETQICRPFIERQIELVN
                     PLVLVHLGGASAKTLLDTSEGILRLRGSWRDYSMASGATIPAMPTLHPAYLLRTPNHK
                     KLAWRDFLEVKAKLAALGR"
     gene            complement(4439..5338)
                     /locus_tag="VGM46_11505"
     CDS             complement(4439..5338)
                     /locus_tag="VGM46_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018426987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY4193261.1"
                     /translation="MALKVLSVAIFVAMSSFIKAAGEVPAGQIVFYRSFFAIFPILFF
                     LGCRRQLRTAFVTERALGHVARGLIGVCAMGLTFFALTRLPLPDAITLNYAQPLLVVV
                     FSSLFLGEAIRFYRWSAVMVGLVGVIIISWPELTLLTSGSALGDQEVLGVMAAFGGAG
                     CSAVAAVLVRNLVHSEKTATIVLWFSIVASVVALFSLPFGWLMLTPMQTVFLVAAGFC
                     GGVAQIFMTSAYRHAEVSTVAPFEYTSMILAIAVGYFFFGDVPTLYMLVGGLIVVGAG
                     LFIILRERQLGLERTKASKAASV"
BASE COUNT         1019 a         1755 c         1668 g          930 t
ORIGIN      
        1 gcgcgctgca tccggcgctc gattccttct cgcatttgcg cgctcacctt gccgtcctgc
       61 tggcgcttct ggcgctggcg ctgctggcgt tcgcggggtt tcgcaccgag gcggcggtag
      121 cgctgctgtt tgccttcggc gctttttcga ccacctccag cgcgctatcg cttccgtggc
      181 tttcgccggt ccaggccggt ttcgtgtcgg ggccgcccga gcgcgccgcc taccgcctgt
      241 tgcagatgaa cctgcgcttt gacaatcgaa cgcctgaaaa agtgctgtcg ctgatcggcc
      301 gcacccaacc tgacgtcatc acgctcgatg aagtttccgt catgtggaaa gccaagttcg
      361 agcttctggc cagcgcctac ccgcaccgca tcctgtgccc ctaccccgac cgaccgttcg
      421 gcgtagccat attatcgcgg cggcctttcg ttgcgggcac ggcgccgcgc tgcttcacgc
      481 ggggggcgct ggccattgca accgtcgatt tcggcggcgc gccggtcgat atcgccgcca
      541 tccacatcgg ctggccgtgg cccttcgaac agggatggca ggtgggcgaa ttgacttcgc
      601 atctcgacct gctgggcgag acggccctga tggcgggaga ttgcaacgcg gtgccgtgga
      661 gcgccaccgt gcgaacggtg gccaaggccg gcgggctgaa gctgatgcct tcggtcggcc
      721 cgacctggca atatatgaag ctgccggatt tcctgcgctt tgccggcctg ccgatcgacc
      781 aggtgttcag caaaggcgcc gtgctgatcc attccgcctc gcggctggaa gacaccggct
      841 cggaccacct gcccgtcctg gtcgaattct cgctgcgccc gcagccggac cagcccgacg
      901 acgaacatgc gaccgcgaca gcctccgccg ccgtttcgcg ccggatgtag cttttgccgc
      961 caggcgcgat gacgcctgaa agccgtttgg ggctacaccc tacatattct ggtagaccgg
     1021 tccttcgccg ccctgcggcg gcacccaggt gatgttctgg ttggggtcct tgatgtcgca
     1081 ggttttgcag tgcacgcagt tctgcgcgtt gatgacgaac cgaacgtcct tggccgaggg
     1141 atcggaggcg gcgttgccgt ccgcatccac ccactcgtaa acgccggccg ggcaatagcg
     1201 gctcgacggc ccgccgaaaa cgtcatgctc ggaacgtttc tgcaactcca tgtcggcgac
     1261 cagcaggtga accggctcgt tctcctcgtg attggtgttc gacaggaaca cggaagacag
     1321 ccggtcgaag gtcaggacgc catcgggctt ggggtagacg atcttcttgt gctgcgaggc
     1381 cggctccagc gaggcagcgt cggtcttgcc gtgcttcagc gtgccgaagg gcgaaaagcc
     1441 gagcaacgtg ttcagccaca tgtccagccc gccgacagcg acgccgacga tggtgccgaa
     1501 gcgcgaccat agcggcttga cgttgcgcac catcttcaaa tccttgccga tggctgtgcc
     1561 gcgccatgcc gcttcataag acgaaagctc gtcatgcgcg cgcccggcgg caacggcttc
     1621 ggcgacgtga tctgccgcca gcatgcccga cagcaccgca ttgtgcgaac ccttgatgcg
     1681 cggcacgttg accagccccg ccgcgcagcc caccagcacg ccgcccggaa acgacagttt
     1741 cggcaccgat tgccagccgc cctcggtgat ggcgcgcgcg ccatagccaa gccgcttgcc
     1801 gccttcgaac gtgccgcgaa tggccgggtg cgtcttgaaa cgctggaatt cctcgaaagg
     1861 cgaaagccag gggttcttgt agttcaggtg caggacgaag ccgaccgcca ccatattgtc
     1921 ttcgagatgg tagaggaacg agccgccgcc ggtcttcatg tcgagcggcc agccgaagga
     1981 gtgctgcaca aggcccggct tgtggttttc cggcttgatc tgccaaagct ccttcaggcc
     2041 gatgccgaac ttgccgggat cgcggccttc cgagaggttg tatttggcga tcagctgctt
     2101 ggccagcgaa ccgcgcgcgc cttcgccgat cagcacatat ttgcccatca gcgccatgcc
     2161 cggcgcaaag gacgggccgt gcgagccgtc cttctcgacg cccatgtcgc cggtaacgac
     2221 gccggttacc gcacccttgt cgtcataaag aagaccgacg gcggcaaagc ccggataaat
     2281 ctcgacgccc agcgcctcgg ccttgccggc cagccagcgg cagacattgc ccagcgagac
     2341 gatgtagttg ccgtggttgt tcatcagcgg cggcatcatg aaattgggca ggcggatcga
     2401 gccggccggg cccagcacca gaaaatggtc ggcggtaacc ggcgtcttga acggatggtc
     2461 gccatcctcg cgccaacccg gcagcaaccg gtcgatgccg atcggatcga cgacggcccc
     2521 ggacaggata tgggcgccaa cctcgccgcc cttttccaaa acgacgacgg aaagctcggg
     2581 gttgatctgc ttgagacgga tggctgccga caggccggcc ggccccgcgc cgacgatgac
     2641 cacgtcgaat tccatgctct cgcgttcaat gtcgctcata gttcctcccg ctggcctcag
     2701 ctgatgcgca tcttgctggc ttatgcggtc catagcgcat caaacggcga cgggaaaccg
     2761 gcgtaaacgt gacaaattgc caattccaac cgcaggccgc cgcgcagcca cgccgttaac
     2821 cgatgtctat ccggcttcgt tctattcagc catactcgcc ctatgcgcat tgacgagttg
     2881 gcttgccttt ctccgacgcg cctttcactc gcgccgggca aggccgcctg cgcgacgctg
     2941 ctttttctgt gtttaatgct gccttttccg gcaaacggac agcaaatgca gacgtggccg
     3001 gccgggcctt attcgttttc cgacgaactg ggcggcttcc ggataacggc ggtttcgggc
     3061 cgaggcaccc atgacgaccc ggtcgtcatc caggaagagc tgaattcggc aacgccggtc
     3121 acgcttaccg ttcgcacgac aagaccgatc cggccattcg cctcttccgg cgactatgcc
     3181 aacggcattc tctacatgcg catgcgggtg ttgaacaata gcggccaggc ctggatagaa
     3241 ttcgagttcg agctacagga aatcctgggc cagcccagcg tattcggcga cggcctgtcg
     3301 ttcgaccagc gcaacaagaa cccgacgaat atcgtctcca gcaattttgc cgggttcgac
     3361 cgcgatttcg agccttacga ccggctgctg ttcaaaaagg gcgctatcga cccgctgaag
     3421 ttagccagct tcgatttcct gatcaccgat tatacgccgc gctggacgtt ctatctggtg
     3481 caggacccgc gcattccctc cagctgagct tgcaaatcgg tggcggcggc gcgaatgtgc
     3541 cggcatgaat gccgcctccc cgaacaaccg gccagatttg cgtgaattgc tggcttttta
     3601 cgccgaagcc ggggtcgacg agccgttgac tgacgagccg gtagaccgtt tcgcgcaagt
     3661 gcccgcgcca aggtcggagc aaggccgacc atccgttgcg gcggcggtgg agcgcgccgg
     3721 cgaagcagac ggcaggcccg aactttccgg cgaaaaccgg acgaatgagc cgccgcgagc
     3781 agcccagcgc cccgccacgc ccaaggccgc catccccgat gaagcgcagg ccggcctggc
     3841 gcgcgaactg gcggcgtctg ccacgacgct tgccgaactt cgcgaagcga tggcttcgtt
     3901 cgaaggctgc aatttgaagc tcacggcaaa gaacatggtg tttgccgatg gcgacccgca
     3961 agccaggctc atgctggtgg gcgaagcgcc gggacgcgac gaggacatag aaggccggcc
     4021 ctttgtcggc cgttccggcc agttgctcga ccggatgctt gcggcaatcg ggatcgaccg
     4081 catgtcggtc tatatcgcca atgtcatccc gtggcggccg ccgggaaacc gcacgccgac
     4141 accgcaggaa acccagatct gccgcccctt catcgagcgg cagatcgagc tggtcaaccc
     4201 gctggtgctt gtgcatctgg gcggggcgtc ggccaagacg ctgctcgata cgagcgaagg
     4261 cattctgcgc ttaaggggaa gctggcgcga ttacagcatg gcttcgggag cgaccattcc
     4321 ggcaatgccg acgctgcatc cggcctatct tctgcgcacg cccaaccaca agaagctggc
     4381 gtggcgcgac tttctggagg tgaaggcgaa gctcgcggcg ctgggccgct gatgccggct
     4441 ataccgaggc ggccttgctg gccttggtgc gctcaaggcc aagctggcgc tcgcgcagga
     4501 tgatgaacaa gccggcgccg acgacgatca gcccgccaac cagcatgtag agcgtcggaa
     4561 cgtcgccgaa gaagaagtaa ccgaccgcga tggccaggat catcgaggta tattcaaacg
     4621 gcgcgaccgt cgaaacttcg gcatggcggt aggcggaggt catgaagatt tgcgccacgc
     4681 cgccgcaaaa gccggcggcc accagaaaca ccgtctgcat cggggtcagc atcagccagc
     4741 cgaacggcag cgaaaacagc gccacgacac tggccacgat ggaaaaccac agcacgatcg
     4801 tcgccgtttt ctcgctgtgg acgaggtttc gcacaagaac ggcggcgacc gccgaacagc
     4861 cggcgccgcc gaaagcggcc atcacgccga gcacttcctg atcgccgagc gccgaaccgg
     4921 aggtcaaaag cgtcagttca ggccatgaaa tgatgatgac gccgacaaga ccgaccatga
     4981 ccgcgctcca gcggtagaag cggatcgcct cgccaagaaa caacgagctg aacaccacca
     5041 cgagcagcgg ctgcgcatag ttcagcgtga tcgcgtcggg cagcggcagc cgcgtcagcg
     5101 caaagaaggt caaccccatc gcgcagacgc cgatcaaccc tcgcgccaca tggccgagcg
     5161 cgcgctcggt gacaaaggcc gtgcgcaatt gacgccggca gccgaggaag aacaaaatcg
     5221 ggaagatggc gaagaacgac cggtaaaaga cgatctggcc ggccggtacc tcgcccgcag
     5281 ccttgatgaa cgacgacatg gcgacgaaaa tcgccaccga caagaccttg agggctatgg
     5341 ccgtaagcgc gccgcgagcg ccgggggccg ta
//
LOCUS       DASYCS010000369         7282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3728191,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000369 DASYCS010000000
VERSION     DASYCS010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 7282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7282
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3728191"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VGM46_11510"
     CDS             <1..251
                     /locus_tag="VGM46_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531834.1"
                     /note="binds to single stranded DNA and may facilitate the
                     binding and interaction of other proteins to DNA; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEY4193262.1"
                     /translation="RKWEKDGVERYSTEVVLQKFRGELQMLDSRGQGGDGGQVSSYSG
                     GGSSRSSDFGQSGPSDNFNRGGGGGGAGSRDMDDEIPF"
     gene            complement(728..3187)
                     /locus_tag="VGM46_11515"
     CDS             complement(728..3187)
                     /locus_tag="VGM46_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY4193263.1"
                     /translation="MNATQAKPVPARARAVIIGGGVSGCSVAYHLAKLGWTDIVLLER
                     KQLTSGTTWHAAGLVGQLRGSKNMTRLAKYSADLYVKLEEETGVGTGMRQVGSISVAL
                     TEERKHEIYRQASLARAFDVDVREISPKEVKEMYPHLNVSDVVGAVHLPLDGQCDPAN
                     IAMALAKGARQRGAKIIEGVKVTAVNTKNGRVTGVTWAQGDEQGTIESDVVINCAGMW
                     ARQVGQESGVNIPLHACEHFYLITEPIPGLTRLPVLRVPDECAYYKEDAGKMMLGAFE
                     PVAKPWGMEGIREDFCFDQLPEDMDHFEPILEMGVNRMPMLGTAGIHTFFNGPESFTP
                     DDRYYLGEAPELGGYWVAAGYNSVGIVSSGGAGMALAQWINDGEPPFDLWEVDIRRAQ
                     PFQKNRLYLKERVTETLGLLYADHFPYRQVATARGIRRSPIHEHLKARGAVFGEVAGW
                     ERANWFAKPGQEREYRYSWKRQNWFDNNRDEHLAVRNNVGLFDMTSFGKIRVEGRDAL
                     SFLQRLCANDLDVAPGKITYTQMLNARGGIESDLTVSRFSETAFFLVVPGATLRRDLA
                     WLRKHLRDEFVVITDVTASESVLCLMGPNARSVIQKISPNDFSNENHPFGTFQEVEIG
                     MGLARAHRVTYVGELGWELYISSDQTAHVFEALEYAGADVGLKLCGLHTLDSCRIEKG
                     FRHFGHDITDEDHVLEAGLGFAVRTKKGEFIGRDAVLRKKDAGLSRRLVQFRLRDPQP
                     LLFHNEAILRDGKIVGTITSGNYGHYLGGAVGLGYVPCEGESEADVLGSAYEVEIAGE
                     RFAAEASLKPMYDPKADRVRM"
     gene            complement(3184..4365)
                     /locus_tag="VGM46_11520"
     CDS             complement(3184..4365)
                     /locus_tag="VGM46_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEY4193264.1"
                     /translation="MPKPSSRISGIVPSGKDGWEVHFTAMTRKQAGEPIIMLSVGDHD
                     FDTPTQTVEACVAAVRAGHHHYTQLPGIPALRAAMAKASTRCTGVATTADEVIATPGG
                     QAALYAAVQGVLDDGDHAIVVAPYYATYPGTFRAAGAAFTVVETRAEHGFQPDPKAIE
                     AAVQPNTRAILINTPNNPTGAVYARKVLEGIADICRRHDLWLLSDEVYWTLGGGEHIS
                     PRSLPGMAERTLVINSMSKSHGMTGWRIGWLTGPASTIQLLTNLNLVSTYGLIDFVSR
                     ATVEALENGYGVKEIAERYAGRRTMFLNAVRGMNDITVRGSEGGMYVMLDIAAIEPDD
                     EKFAAALLDKEMVAVMPGSSFGEAAAGHIRISLCQPEATLKEAAGRIRRFASTYRRNA
                     A"
     gene            complement(4368..6821)
                     /locus_tag="VGM46_11525"
     CDS             complement(4368..6821)
                     /locus_tag="VGM46_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010969823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY4193265.1"
                     /translation="MAELPARARVVIIGGGAVGASSLYHLAKAGWSDCVLLEKNELTS
                     GSTWHAAGNVPTFSTSWSLMNMQRYSTELYRGLADAVGYPMNYHVTGSLRLGHSKERM
                     REFERAKGMGRYQGMDIEVVAADEIKRRYPFVETHDLQGALYDPNDGDIDPAQLTQAL
                     AKGARDLGAKIIRFCPVTGVRRENDEWVVETAQGEIRCEIVVNAAGYRAAEVGRMFGR
                     DVPMMVMSHQYILFEEIPELAAWSREQGKKLPLLRDVDTSWYLRQEKNGMNLGPYEKN
                     CRAHWATADDPMPDDFSFQLFPDDLERLEWYLNDATERVPILGTAGLSKMINGPIPYA
                     PDGNPLIGPMPGVPNAFEACVFTFGISQAGGAGKVLAEWITEGQTEWDMWSCDPRRFT
                     AFAADPAYSLAKGKEIYGNEYAIQFPRRTWPDGRGMKLSAIHDRIEALGAQFGVANGW
                     ERANWYAKPGDDTSDEATQTFARSGPWQQRVREECLAVRDAAGILDLPGFSRYRLQGE
                     GAREWLSQQITGLVPKPGRIGLGYFADDKGRIVTEMSIMALEENLFFLITAASAELHD
                     FEWLKNHLPQGTEMTLQNMTEAFSCQILTGPNTRKILAELADADLSLPWLSHQSAKIA
                     GRWCQLVRVSFAGELGWEIHSKIADTPAIFDAVWQAGQKHGLKPFGMFALDSLRLEKG
                     YRAWKQDLSTDYTILQGGMDRFVKWEKPDFRGKAALAGEKQQGVKKRFVTLTLESPGD
                     CDAPYMATLWHGGKIVGETLSGGFGHRVDKSIALGMLRSDLSEPGTEIEVEIFGDRFK
                     AIVQKDEPLWDPKNERLRA"
     gene            complement(6841..>7282)
                     /locus_tag="VGM46_11530"
     CDS             complement(6841..>7282)
                     /locus_tag="VGM46_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019858736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY4193266.1"
                     /translation="ERGLSMPSLGDLRALAELLDVPVSLFFSHDVPEEQERGLVVRAA
                     RRRSLGTSESGLVEELLSPDLGGSFEMLRSVFAPGAELKAAASRPTEEAGYIVSGTFD
                     IEIAGVWHRLGAGDSFRFEAKPFRWRNPGSEPAVVIWVVSPPVY"
BASE COUNT         1313 a         2227 c         2328 g         1414 t
ORIGIN      
        1 cccgcaagtg ggaaaaggat ggcgtggaac gctattcgac ggaagtggtg ttgcagaaat
       61 tccgcggtga attgcagatg ctcgattcac gcgggcaggg cggcgacggc ggccaggtca
      121 gcagctattc gggcggaggt agcagccgca gctccgattt cggacagtcc ggcccgagcg
      181 acaatttcaa tcgtggcggc ggcggtggcg gcgccggctc gcgggatatg gacgacgaaa
      241 tcccgttctg atccacgggt gttgaagacg aaatcggggc tatgaggtcc tggttccggc
      301 gtggcggggt cgcgtcaagg ttttcaggct ggcttccggg gaaccgtgcc tgaaagagta
      361 gatgccgcgc cggcgcggta attccggcga caaggaaatg gaaatcacct gcgccaagga
      421 caagccggcg ctggcaaccg cgcaaggctg cccgaactgg ccaagagccg tctcgagcgg
      481 atctagtttt taccgaatcg gtaaaaccgc tctatctctt tggtttggcc gcactttcag
      541 caaaaccgtt tcacattttt gctggaaatg ctctgggacc gatatcgccg tcacgcgtgg
      601 ccggcgattt gatggcgctg aagcacgcct gccgcgatcg gcgcatccac agcacgcagg
      661 cgtccgaagc aggatacggc aggattttgc atccgcattt cattttgcgg atcttcgtaa
      721 agcgaaccta catgcgtacc cggtccgctt tcggatcgta catcggcttc agcgaagcct
      781 cggcggcaaa gcgctcgccg gcgatttcga cctcataggc ggaacccagc acatcggctt
      841 cgctctcgcc ttcgcagggc acatagccca ggccgaccgc gccgccgaga taatggccat
      901 aattgccgct ggtgatagtg ccgacaatct tgccgtcacg caggatggcc tcgttgtgga
      961 acaatagcgg ctgcggatcg cgcaggcgga actggacgag gcggcgcgac aggcctgcat
     1021 ctttttttcg cagcacggcg tcgcggccga tgaactcgcc tttcttcgtc ctgacggcaa
     1081 agccaaggcc cgcttccagc acatggtcct catcggttat atcgtggccg aaatggcgga
     1141 aacccttttc gatacggcac gaatccagcg tatgcaggcc gcaaagcttc aggccgacat
     1201 ccgcgccggc atattccagc gcctcgaaaa catgcgctgt ctgatctgac gatatgtaga
     1261 gttcccagcc caattcgccg acataggtaa cgcggtgggc gcgggccagg cccatgccga
     1321 tctcgacctc ttgaaacgtt ccgaacgggt ggttttcatt ggagaagtcg ttcgggctta
     1381 tcttctggat gacgcttctg gcattcggcc ccatcaggca caggacgctt tcggacgccg
     1441 tcacatcggt gatgacgacg aattcgtcgc gcaggtgttt gcgcagccag gcaaggtcgc
     1501 gccgcagcgt cgcgcccggc accaccagga agaaggcggt ctcggaaaag cgggaaacgg
     1561 tcaggtcgct ttcgatgccg ccgcgtgcgt tcagcatctg ggtataggtg atctttcccg
     1621 gtgctacgtc gaggtcgttg gcgcaaagac gttgcaggaa ggagagcgcg tcgcggcctt
     1681 ccaccctgat cttgccgaat gaagtcatgt cgaacaggcc gacattgttg cgaaccgcca
     1741 ggtgttcgtc gcggttgttg tcgaaccagt tctgccgctt ccatgaatac ctatattcgc
     1801 gctcctgtcc gggcttggcg aaccagttag cgcgttccca gccggcaact tcgccgaaca
     1861 ccgcgccacg cgccttcaga tgctcgtgga taggcgaacg gcgtatgccg cgcgcggtcg
     1921 ccacctgccg gtaagggaaa tggtcggcat agagcaggcc gagggtctcg gtgacacgtt
     1981 ccttgagata gagacggttt ttctggaaag gctgggcgcg gcggatatcg acctcccaca
     2041 ggtcgaaggg cggctctccg tcattgatcc actgcgccag cgccattcca gcgccgccag
     2101 aagagacgat gccgaccgaa ttatagccgg cggccaccca gtagccgcca agttcgggcg
     2161 cttcgcccag atagtagcgg tcgtcgggcg taaaactttc cggaccgttg aagaaggtgt
     2221 ggatgccggc agtgcccagc atcggcatgc ggttgacgcc catttcgagg atcggctcga
     2281 aatggtccat gtcctcgggc aactggtcga agcagaaatc ttcgcggata ccctccattc
     2341 cccacggttt cgccacgggt tcgaaggcgc ccagcatcat cttgccggcg tcttccttgt
     2401 aataggcgca ctcgtccggc acgcgcagca ccggcaggcg ggtcaggccg gggatcggct
     2461 cggtgatgag gtagaaatgc tcgcaggcgt gcagcggaat gttgacgccg ctttcctgtc
     2521 cgacctgcct tgcccacata ccggcgcagt tgatcaccac gtcgctctcg atcgtgccct
     2581 gttcgtcgcc ttgcgcccag gtaacgccgg ttacgcggcc attcttggtg ttgacggcgg
     2641 tgactttgac gccctcgatg atttttgcgc cgcgctggcg cgcgcccttg gccagcgcca
     2701 tggcgatgtt ggccgggtcg cactggccgt cgagcggcag gtgcaccgcg ccgaccacat
     2761 ccgaaacgtt gagatgcggg tacatctcct tgacttcctt cggcgaaatc tcgcgcacat
     2821 cgacgtcgaa ggcgcgggcg agcgatgcct gccggtaaat ctcgtgcttg cgttcctcgg
     2881 taagggcaac gctgatcgag ccgacttgcc gcatgccggt gccgacaccg gtttcctctt
     2941 cgagcttgac gtacaaatcg gcggaatatt ttgccagccg ggtcatgttc ttcgagccgc
     3001 gcaactggcc gaccaggccg gctgcatgcc atgtcgtgcc gctggtcaac tgcttgcgct
     3061 caagcagcac gatgtcggtc cagccaagct tggccagatg ataagcgacc gaacaccccg
     3121 agacgccgcc gccgatgatg acggcccgcg ccctggcggg caccggcttt gcctgggtgg
     3181 cgttcatgcg gcatttctcc gataggtcga ggcaaagcgg cggatgcgtc ctgccgcttc
     3241 cttcagcgtc gcttccggct ggcaaagact gatgcggata tggccggccg ccgcttcgcc
     3301 gaagctggaa cccggcatca cggccaccat ttccttgtcg agaagcgccg ccgcgaattt
     3361 ctcgtcgtca ggctcgatgg cggcgatatc gagcatcaca tacatgccgc cctcggaacc
     3421 gcgcacggtg atgtcgttca tgccgcgcac ggcattcaga aacatggtgc ggcggccagc
     3481 atagcgttcg gcgatttcct tgacgccgta gccgttttcc agcgcctcga ccgtcgcgcg
     3541 cgagacgaaa tcgatgagac cgtaggtgct gacgagattg aggttggtca gaagctgaat
     3601 ggtcgaggcg gggccggtca gccagccaat gcgccagccc gtcatgccgt ggctcttgga
     3661 catcgaattg atgaccagcg ttcgctcggc catacccgga agcgagcgcg gcgagatatg
     3721 ctcgccgccg ccaagcgtcc agtaaacctc gtcggaaagc agccacaggt cgtgccggcg
     3781 acagatgtcg gcgatgcctt ccagcacttt gcgggcgtag acggcgccgg tcggattatt
     3841 cggcgtgttg atgaggatgg cgcgggtgtt gggctggaca gccgcctcga tcgccttcgg
     3901 gtccggctgg aaaccgtgct cggcgcgggt ctcgaccacg gtgaatgccg cgccggcggc
     3961 gcggaacgtg ccggggtagg tggcgtaata gggcgcaacc acgatcgcgt ggtcgccgtc
     4021 gtcgagcacg ccctgcacgg ctgcgtaaag cgcagcctga ccgcccggcg tagcgatcac
     4081 ttcatcggcg gtggtggcga cgccggtgca ccgggtcgat gctttggcca tcgccgcccg
     4141 cagggccgga atgccgggaa gctgggtgta gtgatggtgg ccggcgcgca ccgccgccac
     4201 gcaggcttcc accgtctgcg tgggcgtgtc gaaatcgtga tcgccgaccg acagcatgat
     4261 gatcggctcg ccggcctgct tgcgggtcat ggccgtgaag tggacctccc agccatcctt
     4321 gccggaaggc acgatgccgg aaatgcgcga tgatggtttg ggcatgctta ggctctcaat
     4381 ctttcgttct tcgggtccca gagcggttcg tccttctgga cgatagcctt gaagcggtca
     4441 ccaaaaatct cgacctcgat ctcggtgccg ggctcgctca ggtcagagcg cagcatgccg
     4501 agcgcgatcg acttgtctac ccggtggccg aaacctcctg acagggtttc gccgacaatt
     4561 tttccgccgt gccagagggt tgccatgtag ggcgcgtcgc aatcgccggg gctttcgagc
     4621 gtcagcgtga cgaagcgctt tttgacgccc tgctgctttt cgccggcaag ggcggccttg
     4681 ccgcgaaaat cgggcttttc ccatttgacg aagcggtcca tgccgccttg cagaatggta
     4741 tagtcggtgg aaaggtcctg cttccaggcg cggtagcctt tttcaagccg tagcgaatcc
     4801 agcgcgaaca tgccgaatgg cttcagtccg tgcttctgcc cggcctgcca gaccgcgtcg
     4861 aaaatagcgg gcgtatccgc aattttgctg tggatttccc agccgagttc tccggcgaag
     4921 gaaacgcgca ccagctggca ccagcgcccg gcgattttag ccgattggtg cgacagccat
     4981 ggcagcgaaa ggtccgcgtc ggccagttcg gccaggattt tgcgggtgtt cgggccggtg
     5041 agtatctggc aggaaaatgc ctccgtcatg ttttgcagag tcatttccgt gccttgcggc
     5101 agatggtttt taagccactc gaagtcgtgc aactcggccg acgctgccgt gatcaggaag
     5161 aacaggtttt cctccagcgc catgatcgac atttcggtga cgatgcggcc cttgtcgtcg
     5221 gcgaaatagc caaggccgat acggccgggc ttcggcacca ggccggtgat ctgttgcgac
     5281 agccactcgc gcgcgccttc gccttgcagc ctgtagcggg agaagccggg caggtcgaga
     5341 atgcccgcgg cgtcgcgcac ggcaaggcat tcctcgcgca cacgctgttg ccacgggccg
     5401 gagcgggcga aggtctgggt cgcttcgtcg gaagtgtcgt cgccgggttt ggcataccag
     5461 ttggcgcgtt cccagccatt ggcgacgccg aattgcgcgc caagcgcttc gatccggtcg
     5521 tggatggcag aaagtttcat gccgcggcca tccggccagg ttcggcgggg aaactggatc
     5581 gcatattcat tgccgtagat ttctttgccc ttggctaagc tataggccgg atcggcagcg
     5641 aaggcggtga agcggcgcgg gtcgcacgac cacatgtccc attcggtctg gccttcggtg
     5701 atccattcgg ccagcacctt gccggcgccg ccggcctgcg agatgccgaa ggtgaacacg
     5761 caggcctcga aggcgttggg cacgcccggc atcgggccga tcagcgggtt gccgtccggt
     5821 gcgtatggga tcggcccgtt gatcatcttg gacaggccgg cggtgccgag gataggcacg
     5881 cgctcggtgg catcgttgag ataccattcc agccgctcca gatcatccgg gaaaagctgg
     5941 aacgaaaaat cgtcgggcat cggatcgtcg gcggtcgccc aatgcgcgcg gcaattcttc
     6001 tcataggggc cgagattcat gccgttcttc tcctggcgca gataccagga ggtatcgacg
     6061 tcgcgcagca gcggcaattt cttgccctgt tcgcgcgacc acgcggcaag ttcggggatt
     6121 tcctcaaaca ggatatattg gtggctcatc accatcatgg gcacgtcacg gccgaacatc
     6181 ctgccgactt ccgccgcgcg gtagccggcg gcgttgacga cgatttcgca gcggatttcg
     6241 ccttgtgcgg tttccaccac ccattcgtcg ttctcacggc gaacgccggt aaccgggcag
     6301 aagcggatga tctttgcgcc cagatcgcgc gcgcccttgg ccagcgcctg cgtcaactgc
     6361 gcgggatcga tgtcgccatc gttgggatcg taaagcgcac cctggaggtc gtgggtttca
     6421 acgaaggggt aacggcgctt gatctcgtcg gcggcgacga cctcgatatc catgccctgg
     6481 tagcggccca ttcccttggc gcgctcgaat tcgcgcattc gctccttgct gtggccaagg
     6541 cgcagcgagc cggtgacgtg gtagttcatc gggtagccga cggcatccgc cagtccacgg
     6601 taaagctcgg tcgaataacg ctgcatgttc atcagcgacc acgaggtcga gaaagtcggc
     6661 acgttgccgg cggcatgcca ggtggagcct gaagtcagct cgttcttctc cagaagcacg
     6721 caatcgctcc agcctgcctt ggcaaggtgg tagagcgagg acgcgccgac cgcgccgccg
     6781 ccgatgatga cgacacgggc ccttgccggt aactcggcca tcgataactc ctccaagcgt
     6841 tcaatagacg ggtggcgaaa ccacccagat gacgactgcc ggctcactgc cgggattgcg
     6901 ccagcggaaa ggctttgcct cgaaacggaa gctgtcgccc gcgccgagcc gatgccacac
     6961 accggcgatt tcgatatcga acgtgccgga tacgatgtag ccggcttcct cggtcgggcg
     7021 gctggcggcg gccttcaatt cggctccggg cgcgaaaacg gagcgcagca tttcgaagct
     7081 gccgccgaga tctggcgaca ggagttcttc gaccagtccg gattcactgg tccccagcga
     7141 gcgccgccgc gcggcgcgaa caactaggcc gcgctcctgc tcttcaggca catcgtggct
     7201 gaagaacagg ctgaccggca cgtccagcaa ttccgccaag gcccgcaaat cgcccagcga
     7261 gggcatggaa agaccacgct cg
//
LOCUS       DASYCS010000370         4969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_683487,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000370 DASYCS010000000
VERSION     DASYCS010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4969
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683487"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="VGM46_11535"
     CDS             <1..785
                     /locus_tag="VGM46_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY4193267.1"
                     /translation="PLDSISIVHGDTDKVQMGMGTYGSRSGAVGMSAIVKALDKVEAK
                     ARKIAAHLMEADEADIVIEDGALKVAGTDKSVPWFQMALAAYTAHNLPAGMEPGLKET
                     AFYDPANFTFPAGCYICEVEIDPDTGHTDIVQFVAADDFGNIINPMIVEGQVHGGVAQ
                     GVGQALLEGANYDAGNGQLLTASFMDYTMPRAGDLPSFKVSTTNTPSPSNPLGLKGCG
                     EAGAIGSPPAVINAITDAIGTEKITMPATPQKVWAAIRAAKH"
     gene            799..1593
                     /locus_tag="VGM46_11540"
     CDS             799..1593
                     /locus_tag="VGM46_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEY4193268.1"
                     /translation="MYSVNYHRATSVADAVKLLKTGDARPLSGGMTLIPAMKTRLAAP
                     SDLVDLAHVKELKGIKVSGKTVTIGAGTTHFEVANDEKLKKACPALAHLASLIGDPAV
                     RHKGTIGGSIANNDPAADYPAAMLALGATIVTDKREIAADKFFKGLFETALKDGEIVT
                     AVSFMAPAKAAYQKFRNPASRYAIVGVFVAKGKEVSVAVTGAGDDGVFRSKEIEAALS
                     KRFEPSALEGLAVPANGLMSDMHASADYRASLIVVMAKRAVAAANG"
     gene            1768..3159
                     /locus_tag="VGM46_11545"
     CDS             1768..3159
                     /locus_tag="VGM46_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEY4193269.1"
                     /translation="MATETREDLSWFSRARTIARPGYSRWMVPPAALCVHLCIGQAYA
                     YSVFNLPMTKLIGITDSAPDDWKLTELGWIFSLAIFFLGVSSALFGRWVEEGGPRKAM
                     FTAALCWTGGFLVSALGVYLHMLWLIYLGYGVLGGIALGIGYISPVSTLIKWFPDRPG
                     MATGMAIMGFGGGAFIASPLSVWLMKKFTTESHIGVMETFIILGIVYFVFMMVGSIIV
                     RVPPDDWKPEGYVAPKATKLITSNNVYVYDALKTPQFWLIWVVLCVNTTAGIGVLGQA
                     SAMSQEMFPDRITPIAAAGLVGLMSLFNMGGRFAWATTSDYLGRRNTYFIFMVLGFIL
                     YCLVPYMGGVGSIAGFVLCFCIIISMYGGGFATVPAYLRDMFGVRYVGAIHGLLLTAW
                     SAAGIFGPVLVNYIREYNVTHNVPKTDAYNTTMYIMAGLFVLGFICNFFVKAVHERHH
                     MKAQDAALSAVAG"
     gene            3177..3302
                     /locus_tag="VGM46_11550"
     CDS             3177..3302
                     /locus_tag="VGM46_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193270.1"
                     /translation="MNDQDHVATTPLHLVLAWGFVGIPLVWGIFQTLLNALQLFK"
     gene            3671..4630
                     /gene="dusA"
                     /locus_tag="VGM46_11555"
     CDS             3671..4630
                     /gene="dusA"
                     /locus_tag="VGM46_11555"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006333697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HEY4193271.1"
                     /translation="MMDWTDRHCRFFHRQLTRQALLYTEMVVADAVIHGARQRLLGFD
                     AVEHPVALQLGGSDPQKLAEAARIGESFGYGEINLNVGCPSDRVQSGTFGACLMKTPA
                     LVAQCVAEMKAAVKIPVTVKCRIGVDDQNPEQALDELADGVFAAGADAVWVHARKAWL
                     EGLSPKENRDVPPLDYDRVYRLKARMERRFIGINGGVQTVDEVRNHLHHVDGVMLGRA
                     AYHTPGMLTAVDAAVYGEPPRDFDYPQLIETMAAYATRHIEKGGRLGHVTRHMVGLFH
                     GLPGARRWRQILSTEATRPGAGAEVLKAAFAAVDLDGAAEQAA"
     gene            complement(4635..>4969)
                     /locus_tag="VGM46_11560"
     CDS             complement(4635..>4969)
                     /locus_tag="VGM46_11560"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006333695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HEY4193272.1"
                     /translation="FEARLIVNGAAIRAVVDTGASSTVLSADDAEAAGLDPDSLKYTV
                     PVATANGGTNAAGARTRELSIGGIVRMNMPVLVAAPGTLEQSLLGMNFIGTLSGFDVR
                     GDRMILRD"
BASE COUNT          937 a         1467 c         1513 g         1052 t
ORIGIN      
        1 tgccgctcga ttccatctcc atcgtccatg gcgacaccga caaggtgcag atgggcatgg
       61 gcacttacgg gtcgcgttcc ggcgcggtcg gcatgtcggc catcgtcaag gcgctcgaca
      121 aggtggaggc caaggcgcgc aagatcgccg cgcatctgat ggaagccgac gaggccgata
      181 tcgtcatcga ggacggcgcg ctgaaagtgg ccggcaccga caaatcggtg ccatggttcc
      241 agatggcact ggccgcctat acggcgcaca atctgccggc cggcatggag ccgggcctca
      301 aggaaaccgc tttttacgat ccggccaatt tcaccttccc ggcgggctgt tacatctgcg
      361 aggtggaaat cgatcccgat accggccaca ccgatatcgt ccagttcgtg gctgccgacg
      421 atttcggtaa tattatcaat ccgatgatcg tggaaggtca agttcatggc ggtgtcgcgc
      481 aaggcgtcgg gcaggcgttg ctggaaggcg ccaactacga tgcgggaaac ggccagcttc
      541 tgacggcgag tttcatggac tacaccatgc cgcgcgccgg cgacctgccg tcgttcaagg
      601 tatcgaccac caacacgccg tcgccgtcca acccgctcgg cctcaagggc tgtggcgagg
      661 cgggggcgat cggatcgccg ccggcagtca tcaacgcgat caccgatgcg atcggcaccg
      721 agaagatcac catgccggcc acgccgcaaa aggtttgggc cgcgatccgg gcagcgaagc
      781 actgaggagg agacagccat gtattccgtc aattatcacc gcgccacctc ggttgccgac
      841 gccgtaaagc tgctcaagac cggcgatgcc aggccgctat cgggcggcat gacgctgatc
      901 ccggcgatga agacgaggct tgcagcgcct tcggaccttg tcgacctcgc ccatgtcaag
      961 gaactgaagg ggatcaaggt ttcgggcaag acggtaacga tcggcgcggg caccacgcat
     1021 ttcgaagtcg ccaatgacga gaaactgaaa aaggcatgtc cggcgcttgc ccatctggcg
     1081 tcgctgatcg gcgatccggc ggtgcgccac aagggcacga tcggcggctc cattgccaat
     1141 aacgacccgg ccgccgacta tccggcggcg atgctggcat tgggcgctac catcgtcacc
     1201 gacaagcgcg agattgccgc cgacaagttc ttcaagggtc ttttcgagac ggcgttgaag
     1261 gatggcgaga tcgtgaccgc ggtatccttt atggcaccag ccaaggctgc ctaccagaaa
     1321 ttccgcaatc cggcttcccg ctacgccatc gtcggcgtgt tcgtcgccaa gggcaaggag
     1381 gtcagcgtgg cggtcacagg cgcgggcgac gacggcgtgt ttcgctcaaa agaaattgaa
     1441 gcggcgctgt cgaaaagatt tgagccgtcg gcgctcgaag ggttggccgt gccggctaat
     1501 ggattgatga gcgatatgca tgcctcagcc gactaccgcg ccagcctgat cgtcgtcatg
     1561 gcaaagcgtg ccgtcgccgc ggcaaatgga tagtataatg aaaacatgaa tttgtgcccg
     1621 tttcatcatg atgacggcac gattcgaagc cggtgcgttg cgccggcttt tttgtgaggg
     1681 aaccgggaat tttttgtatc gcttgccgat ccaattgatt atctttcgca tgttaacaaa
     1741 agttaatcat tatttagggg gagatagatg gcaacggaaa cgcgagagga tctgagctgg
     1801 ttcagccgtg cgcgcacgat agctcgtccg ggttatagcc gctggatggt gccgccggcc
     1861 gcactttgcg tgcatctttg cataggccag gcctatgctt acagcgtgtt caaccttccg
     1921 atgaccaagc tcatcgggat caccgattcc gcgcctgacg actggaagct gacggagctt
     1981 ggctggatat tttcactggc gatcttcttt ctcggcgttt cctcggcgct ctttggccgt
     2041 tgggtcgagg aaggcggtcc acgcaaggcg atgttcacgg ccgcgctctg ttggaccggc
     2101 ggctttctgg tctcggcgct cggcgtctat ctccatatgc tgtggctgat ttatctgggc
     2161 tatggcgtgc ttggcggcat cgcgctcggc atcggttata tttcgccggt atcgacgctg
     2221 atcaaatggt ttccggaccg tccgggaatg gccaccggca tggccatcat gggcttcggc
     2281 ggcggcgcct tcatcgcttc gccgctttcg gtctggctga tgaagaaatt caccaccgaa
     2341 agccatatcg gcgtgatgga aaccttcatc atattgggca tcgtgtattt cgtgttcatg
     2401 atggtcggct cgatcatcgt gcgcgtgccg ccggatgact ggaagccgga aggctatgtc
     2461 gctccgaaag ccacgaaact gatcaccagc aacaatgtct acgtttacga cgcgctgaag
     2521 acgccccaat tctggctgat ctgggtggtc ttgtgcgtca acaccacggc gggcatcggc
     2581 gttctcggcc aggcttccgc gatgagccag gaaatgttcc ccgatcgcat tacgcctatc
     2641 gccgctgcgg ggcttgtcgg cttgatgagc ctgttcaaca tgggcggacg tttcgcctgg
     2701 gcgacgacct cggactatct cggccgcagg aacacctatt tcatattcat ggtgctgggc
     2761 ttcatcctct attgcctggt gccctatatg ggcggcgtcg gcagcattgc cggtttcgtg
     2821 ctctgcttct gcatcatcat ctcgatgtat ggcggcggct tcgcgaccgt gccggcctat
     2881 ctgcgcgaca tgttcggcgt tcgctatgtc ggcgccatcc acggcctgct gcttaccgcc
     2941 tggtcggccg cgggtatttt cggaccggtt ctggtgaact acatccgtga atacaacgtg
     3001 acccacaatg tgccgaagac cgacgcctac aacaccacca tgtacatcat ggcagggctg
     3061 ttcgtgctcg gcttcatctg caacttcttc gtcaaggcag tacatgagcg ccatcatatg
     3121 aaggcgcaag atgctgcatt gtcggcggtt gccggttaag cttcgaggag gatatcatga
     3181 acgatcaaga tcacgtcgcg accacgccgc tgcacctcgt cctcgcctgg ggttttgtcg
     3241 gcattccgct cgtctggggc attttccaga cgctgctgaa cgcgctccag cttttcaaat
     3301 aaagccggtc cttcgacgcc atcctgcgat ggccccatat ctgaaacggc gctggaaacg
     3361 gcgccgtttt tcgttcgccg gtggacctgc cggtcggcat ggcggcatat tttttgcggc
     3421 gaatgaaaag aaatgccccg taaaaattcc gccgtagcaa aaccggcatg atgccggttc
     3481 gctcgcttta tccgggtttg cgatgtatag cggctttttt tactgccttc gcatctgcgc
     3541 gccgccgctt gcggtctctc atgcgtcaac agcaggcgac gaacgaaacg gcgatagtca
     3601 gatgacggaa aatcagcgcc ctggagacaa ggcttttgtc tataaggctc cggttgtggc
     3661 catcgcaccc atgatggact ggacggacag acattgccgt ttcttccatc gccagctgac
     3721 gcggcaggcg ttgctgtata ccgaaatggt cgttgccgat gccgtcattc atggcgcgcg
     3781 ccagagattg ctcggatttg atgctgtgga gcatccggtt gccctacagc ttggcggctc
     3841 cgatccgcaa aagctcgcgg aggcggcgcg cattggtgag tccttcggtt atggcgagat
     3901 caatctcaat gtcggctgcc cttccgaccg cgtacagtct ggcaccttcg gggcttgcct
     3961 gatgaagacg ccggcgctcg tggcgcaatg cgttgccgag atgaaggctg cggtgaaaat
     4021 tccggtcacc gtcaaatgtc ggatcggcgt cgatgaccag aaccccgaac aggcgctcga
     4081 tgaactggcc gacggcgttt tcgccgctgg cgccgatgcg gtgtgggtcc atgcccgcaa
     4141 ggcgtggctg gagggacttt cgccgaagga aaatcgcgat gtcccaccac tcgattacga
     4201 tcgcgtctat cgcctgaagg ccagaatgga gcgtcgcttc atcggcatca atggcggcgt
     4261 tcaaacggtc gatgaggtgc gcaaccatct tcatcatgtc gatggcgtca tgcttggccg
     4321 cgccgcctat catacgccag gcatgctgac ggccgtcgat gccgccgtct atggcgagcc
     4381 gccgcgcgat ttcgactatc ctcagctgat cgaaaccatg gctgcctacg caacacgcca
     4441 tatcgagaag ggtggccgtc ttggccatgt cacccgccac atggtcggct tgtttcatgg
     4501 gctgccgggc gctcgccgct ggcggcaaat tctctccacc gaagcgacac gtccgggggc
     4561 gggcgctgaa gtgctgaaag cagccttcgc ggcggtcgat ctcgacggag cggcagagca
     4621 ggctgcctga cggatcagtc gcgcaggatc atccggtcgc cgcgcacgtc gaagccggac
     4681 aacgtgccga tgaaattcat gccgagcagg ctttgctcaa gcgttcccgg cgcggctact
     4741 agcaccggca tgttcatgcg tacgatgccg ccgatggaca attccctggt gcgtgcgccg
     4801 gccgcgttcg tcccgccatt cgccgtagcc accggcacgg tatatttcag gctgtccggg
     4861 tcgaggccgg cggcttcggc atcatcggcg ctcaacaccg tgctactcgc gccggtatca
     4921 accaccgcgc gaatggccgc gccgttgacg atgaggcgtg cttcgaaat
//
LOCUS       DASYCS010000371         1881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2712527,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000371 DASYCS010000000
VERSION     DASYCS010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2712527"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VGM46_11565"
     CDS             <1..311
                     /locus_tag="VGM46_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006332500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEY4193273.1"
                     /translation="RNHLPETMVKAFEIVGLDRETVEERFGGLYRAFQYGAPPHGGMA
                     AGVDRVVMLLLGAKNLREITMFPMNQQAYDLLMSAPSQATPQQLRELSLRVAVAKKEA
                     "
     gene            complement(358..681)
                     /gene="hspQ"
                     /locus_tag="VGM46_11570"
     CDS             complement(358..681)
                     /gene="hspQ"
                     /locus_tag="VGM46_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019858091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HEY4193274.1"
                     /translation="MKTAKFSIGQVVRHRLFSFRGVIFDVDPEFANTDEWYEAIPADV
                     RPRKDQPFYHLLAENSETEYVAYVSEQNLLEDETGEPVRHPQIREMFEKKPDGRYSPR
                     RQSRH"
     gene            complement(757..>1881)
                     /locus_tag="VGM46_11575"
     CDS             complement(757..>1881)
                     /locus_tag="VGM46_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006333611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiH/UbiF family hydroxylase"
                     /protein_id="HEY4193275.1"
                     /translation="FAVTLIGPDAVADDRRTTALMNPALEMLDSLGVLAQLAEVSAPL
                     KIMRIVDATGRLIRSPLVTFRASEIDEERFGLNFPNRALVATLAAAAAANSAIEWRKS
                     LAASWRIDPEQAVALLANGEEVSAPLAVAADGRNSPARAAAGISTSSRAYPQAALVLN
                     FGHSREHGFISTEFHTETGPFTQVPLPGNRSSLVWVVKPETAKDFVAVDDAALSALIE
                     QKMQSMLGRVAVEPGRQVYPLTVATPARFAQNRIALVGEAAHVFPPIGAQGLNLGIRD
                     IADLVGIIGENRDDPGGARALASYDARRRPDILARSSAVNLFNSSLLSSLLPAQVARS
                     AGLGLLGSLAPLRAFFMREGLRPGSGFAAMSGLFRRGGGL"
BASE COUNT          357 a          603 c          586 g          335 t
ORIGIN      
        1 tccgcaatca cctgccggaa acgatggtca aggcattcga gatcgtcggg cttgaccggg
       61 agactgtcga ggagcgcttt ggcggcctct accgcgcatt ccagtatggc gcgccgcccc
      121 atggcggcat ggcggccggc gtcgaccgcg tggtcatgct gctgctgggc gccaagaatc
      181 tgcgcgaaat caccatgttc ccgatgaacc agcaagccta cgatctgctg atgagcgcgc
      241 cttcgcaagc gaccccgcag cagcttcgcg aactgtcgct gcgcgtagcc gtcgccaaga
      301 aggaagcctg aggccgcaag ccgacccgac cgctggatgc caaaccggcg gtcgggttta
      361 gtgacgcgac tggcgcctgg gcgaatagcg gccgtcgggt ttcttttcga acatttcccg
      421 gatttgcgga tggcgtacgg gctcgccggt ctcgtcttcg agcaggttct gctcggaaac
      481 ataggcgaca tactccgtct cggaattttc ggcgagcagg tggtaaaagg gctggtcctt
      541 gcgcggccgg acatcggcgg gaatcgcctc ataccactcg tcggtgttgg cgaattccgg
      601 atcgacatcg aaaatcacgc cgcgaaatga aaacagccgg tggcgaacca cctgtccgat
      661 tgaaaattta gctgtcttca ttgttacgca ccactgaaac atctgctcta tttgacgcag
      721 acgccgataa atttcaatcc cgcagctttt gtggccctat agaccgccgc cgcgccggaa
      781 caggccggac attgccgcga agccgctgcc gggccgcagg ccctcccgca tgaagaaagc
      841 gcgcagcggc gccagcgagc caagcaggcc aaggcccgcg ctgcgcgcca cctgtgccgg
      901 caacaggctc gaaagcagcg agctgttgaa tagattgacg gcgcttgagc gagccagaat
      961 gtcgggccgg cgtctggcgt cataggaggc cagcgcgcgc gccccccctg ggtcatcgcg
     1021 gttttcgccg atgatgccga ccaggtccgc tatatcccgg atgccgagat taaggccctg
     1081 cgcgccgatt ggcggaaata cgtgcgcggc ctcgcctact agcgcgatcc tgttttgcgc
     1141 aaagcgcgcc ggcgtcgcca ccgtcagggg atagacctga cggccaggct cgacggctac
     1201 ccggccaagc atcgactgca tcttctgttc gatcagcgcc gacaaggccg catcgtctac
     1261 ggcaacgaaa tccttggccg tttccggctt gaccacccac accaggcttg atcggttgcc
     1321 gggcagggga acctgggtaa acggtccggt ctccgtgtgg aactcggtcg agatgaagcc
     1381 atgctcgcgg ctgtggccga aattgagcac aagggctgct tgcggataag cccggctcga
     1441 agtcgatatt ccggcggccg cgcgtgccgg cgagttgcgc ccatccgccg ccacggccag
     1501 gggcgcggaa acctcttcgc cattcgccag cagcgccact gcctgctccg gatcgatccg
     1561 ccacgaggcg gcgagcgact tgcgccactc gatagctgaa ttggcggcgg cagcggccgc
     1621 caaggttgcc acaagtgcgc ggtttgggaa attaagcccg aaccgctcct cgtcgatttc
     1681 gcttgcccgg aacgtaacaa gcggactgcg gatcaatcgg cctgtcgcat cgacgatgcg
     1741 catgattttc aacggagccg aaacctcggc caattgtgca agcacgccca gactgtccag
     1801 catttccagt gccgggttca tcagcgccgt ggtgcggcgg tcgtcggcca ccgcatccgg
     1861 cccgatcaac gttaccgcga a
//
LOCUS       DASYCS010000372         6285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3872434,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000372 DASYCS010000000
VERSION     DASYCS010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 6285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6285
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3872434"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..1250
                     /locus_tag="VGM46_11580"
     CDS             27..1250
                     /locus_tag="VGM46_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4193276.1"
                     /translation="MTDTTATAATTPAQSSAQATALAVILAVSLCHGINDIMQSLLAA
                     IYPLLKQNYGLDFWQIGLLTFTFQMTASMLQPVIGMVTDKKPMPYSLPYGMASSLIGL
                     IVLAYAGHYWLLLVGASLIGIGSAVFHPESSRIARLASGGRHGLAQSLFQVGGNAGQA
                     MGPLLAAFVVVPFGQKSIAWFAIGSMIGIIVLWQVSGWYTRLRAATKHHKAAIYVSPF
                     PKKRVAWALTILTLLVLTKNAYIASLSSYYTFYVIHKFGVSVQTSQVLLFAFLGSSAL
                     GILLGGPFGDRFGQKAMIWFSIVGVLPFTLALPYANFEWTVILTIIIGLILSSAFSNI
                     VVFAQELVPGRVGMIGGIFFGLAFGVGGIAAALLGVVADEMGIDFVFQVCSYLPFLGL
                     LTIFLPNMKEARKAA"
     gene            1470..2102
                     /locus_tag="VGM46_11585"
     CDS             1470..2102
                     /locus_tag="VGM46_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEY4193277.1"
                     /translation="MSQASYELKAEAREQVGKGSARAVRRDGKVPAVIYGDKQPPLAI
                     TLSYKEIFYKIHGGGFLTTVATIDVGGKKIQVLPKDYQLDPVRDFPMHVDFLRVGKNT
                     QVNVNVPVHFINEEKSPGIKRGGVLNVVRHEVEFHCPANAIPEFITVDLDSIDIGASI
                     HISSVKLPEGVKPVISDRDFTIATIAGTSAMKSEEGDASEAAASAAAEEK"
     gene            2124..2867
                     /gene="pth"
                     /locus_tag="VGM46_11590"
     CDS             2124..2867
                     /gene="pth"
                     /locus_tag="VGM46_11590"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEY4193278.1"
                     /translation="MLLFAGLGNPGAQYANNRHNVGFMAADAIARRHSFSPWSKKFHG
                     LIAEGAICGEKAILIKPQTFMNLSGQSVGEALRFYKLTPSAVTVLYDEIDLAAGKLRV
                     KVGGGSGGHNGIRSLDQHIGKEYRRVRIGVGHPGAREMVHGHVLGDFSKADRGWLEVL
                     LDAIAADIDILAKGDDNGFMNRITLALRDKLQPTGEHDTPPPKAPKPPKPAAAQSHIR
                     QARPPQKLAKLPETGPMAAMLKKLFGDEE"
     gene            2975..4078
                     /gene="ychF"
                     /locus_tag="VGM46_11595"
     CDS             2975..4078
                     /gene="ychF"
                     /locus_tag="VGM46_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004691026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEY4193279.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTRTAAAQAANYPFCTIEPNTGE
                     VAVPDPRLKKLAEIGKSKEIIPTRISFVDIAGLVRGASKGEGLGNQFLANIREVDAIV
                     HVLRCFEDDDITHVEGRIDPVADAETVETELMLADLDSLERRIVQFRKRAASKDKEAL
                     TILPVMEGTLALLNEGKPARVMLKNIAPEDLEILRSLNLLTSHPVLYVCNVSEADAAT
                     GNEHTRAVEKMADAQGAGTVVISAAIEAEVAQLPDEEAQEFLADLGLEEPGLDRLIRA
                     GYALLHLITYFTVGPKETRAWTIERGTKAPQAAGVIHTDFERGFIRAQTIAYADFVAL
                     GGEVAAKEAGKARDEGKEYVVQDGDVLLFRFNT"
     gene            4185..4649
                     /locus_tag="VGM46_11600"
     CDS             4185..4649
                     /locus_tag="VGM46_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006338527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEY4193280.1"
                     /translation="MTARKWAFEDFIEGATLDLGSKHVTAEEIVEFASEFDAQPMHLS
                     EEGGRQSILGGLSASGWHTCAMFMRLLCDAILLDSTSQGAPGIEQVKWKKPVLAGDTL
                     TGRSTVLGKRLSKSRPGLGFVSMRAELFNQRGKSVFELENVGMFLSREAVPS"
     gene            4646..5107
                     /locus_tag="VGM46_11605"
     CDS             4646..5107
                     /locus_tag="VGM46_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006338528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEY4193281.1"
                     /translation="MNLDDYFAIGVTTDLGSHKFEPEEIKVFARKYDPQPFHTDEIAA
                     RDSVFGGLCASGWHTTAVWMKRNLATGMDGRWTGPGPKPEFGPSPGFKNLKWLKPVYA
                     GETIRFTRACLGHRPLASRPGWRVLAIRAEAFDSTGDKVIEFENAVLVKTG"
     gene            complement(5180..5725)
                     /locus_tag="VGM46_11610"
     CDS             complement(5180..5725)
                     /locus_tag="VGM46_11610"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEY4193282.1"
                     /translation="MNSSLEATLLAAIRTIPDYPKPGVLFRDITTLLGNASAFRRAVD
                     ELVHPFAGQKVNKVAGIEARGFILGGAVAHQLSAGFVPIRKKGKLPHETVRVAYSLEY
                     GLDEMEMHKDGVAPGEKVILIDDLIATGGTAEAAVKLLRQIGADIIAACFVIDLPDLG
                     GRKKLEALGVPVRTLIGFEGH"
     gene            complement(5821..>6285)
                     /locus_tag="VGM46_11615"
     CDS             complement(5821..>6285)
                     /locus_tag="VGM46_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEY4193283.1"
                     /translation="QAAAAANGGAAPPDMSLLAKARGVERGFPQFVIDIFTQYAAGGP
                     DYIHSLLNGYDQQPPAGMVIPEGTHYNPYFMSGVSLKMPKPLSDGQVTYGDGSPQTVE
                     QYSQDVSAFLMWAAEPHLEDRKKTGFRVVIFLLLFAGLMYATKRKVWADVAH"
BASE COUNT         1198 a         2089 c         1871 g         1127 t
ORIGIN      
        1 cgcatcgcca cgaacggaag cacatcttga ccgacaccac agcgactgcc gcaaccaccc
       61 ccgcccaaag ctcggcgcag gcaactgccc tggcggtcat tctcgcggtc agcctttgcc
      121 acggcatcaa cgacatcatg cagtcgctgc tggcggccat ctatccgctg ctcaagcaga
      181 actacggcct cgatttctgg cagatcggcc tgttgacctt taccttccag atgaccgcct
      241 cgatgctgca accggttatc ggcatggtca ccgacaagaa gccgatgccc tattcgctgc
      301 cctatggcat ggcttcctca ctgatcggcc tgatcgtgct tgcctatgcc gggcactatt
      361 ggctgctgct ggtcggcgcg tcgctgatcg gcatcggctc ggccgtcttc cacccggaat
      421 cgtcgcgcat cgcgcggctg gcctcgggcg ggcggcacgg cctcgcccaa tcgctgttcc
      481 aggtcggcgg caatgccggc caggcgatgg gaccgctgct cgccgccttc gtcgtcgtgc
      541 ccttcggcca gaaaagcatc gcctggttcg ccatcggttc gatgatcggc atcatcgtgc
      601 tgtggcaggt cagcggctgg tacacccgcc tgcgcgcggc gaccaaacac cacaaggcgg
      661 ccatttacgt ctcgcccttc ccgaaaaaaa gggtggcctg ggcgttgacg atcctgacgc
      721 tgctggttct gaccaagaat gcctacatcg ccagcctgtc cagctattac accttctatg
      781 tcatccataa attcggcgtt tccgtgcaga cgtcgcaggt gctgctgttt gccttcctcg
      841 gctcgtccgc acttggcatc ctgcttggcg gcccgttcgg cgaccgcttc gggcaaaagg
      901 cgatgatctg gttttccatc gtcggcgtgc tgcccttcac gctcgctttg ccctacgcca
      961 acttcgagtg gacggtgatc ctgacgatca tcatcgggct gatcctgtcg tcggcctttt
     1021 ccaacatcgt cgtcttcgcg caggaactgg tgcccggccg cgtcggcatg atcggcggca
     1081 tcttcttcgg cctcgccttc ggcgtcggcg gtatcgcagc tgcactgctc ggcgtggtcg
     1141 ccgacgagat gggcatcgat ttcgtcttcc aggtctgttc ctacctgccg ttcctcgggc
     1201 tgctcaccat attcctgccc aacatgaagg aagcccgcaa agccgcctga acttccccgg
     1261 caggcggcgg cgcgcaaggc gggtggccgg cttgcgccat ctgccgcctt ccgttataga
     1321 accgccaccc gcatagacac ccttggaggc aatgcggagg aacgtcgtcc cggtgcatga
     1381 tccgtcatgc gatgccgtgg cggctttcga cgtttccggc tccggcttgc cgcccgaaga
     1441 cgctccaaat aacactgaaa ggaaatgcca tgagccaagc ttcctacgag ctcaaggccg
     1501 aagcacgcga acaggtcggt aaggggtccg cccgtgcagt tcgccgcgac ggtaaagtac
     1561 cagcagtcat ctatggagat aagcagcctc ccctggctat cacgctctcc tacaaggaaa
     1621 tcttctacaa gattcacggc ggcggcttcc tgaccaccgt ggccacgatc gatgtcggcg
     1681 gcaagaagat ccaggttctg cccaaggact accagcttga cccggtccgc gacttcccga
     1741 tgcatgtgga tttcctgcgc gtcggcaaga acacgcaggt caacgtcaac gtgccggtcc
     1801 acttcatcaa cgaggaaaag tcgcccggca tcaagcgcgg cggcgttctc aatgtcgtgc
     1861 gccacgaggt cgagttccac tgcccggcca atgccatccc ggaattcatc acggttgacc
     1921 tcgacagcat cgatatcggc gcctcgatcc atatttcttc ggtcaagctg ccggaaggcg
     1981 tcaagccggt catctccgac cgcgacttca ccatcgcgac cattgccggc acctcggcaa
     2041 tgaagtcgga agaaggcgac gcctccgaag ccgcagcatc cgctgccgcc gaagagaaat
     2101 aatcgctcgc ccggagcgcg gcgatgctgc tgtttgcagg tcttggcaat ccgggcgcgc
     2161 aatacgccaa caaccggcac aatgtcggct tcatggcggc ggacgctatc gcccgccgtc
     2221 attccttttc gccatggtcg aagaaattcc acggcctgat cgccgaaggc gccatctgcg
     2281 gcgaaaaagc catcctcatc aagccgcaga ccttcatgaa tctttccggc cagtcggtcg
     2341 gcgaggcgct gcgcttctac aagcttaccc cctccgccgt gaccgtgctt tatgacgaga
     2401 tcgaccttgc cgccggcaag ctgcgggtca aggtcggcgg cgggtccggc ggccataacg
     2461 gcatccgctc gctcgaccag catatcggca aggaatatcg ccgcgtgcgc atcggcgtcg
     2521 gccatccggg cgcgcgggaa atggtgcacg gccatgtgct gggcgacttt tccaaggccg
     2581 accgcggctg gctggaagtg ctgttggacg ccatcgccgc cgacatcgat atcctggcca
     2641 agggcgacga caacggcttc atgaaccgca tcacgctggc gctgcgcgac aagcttcagc
     2701 cgaccggcga gcacgacacg ccgccgccga aagcgccgaa gccaccaaag ccggccgccg
     2761 cgcaaagcca catccgccag gcccgcccgc cgcaaaaact ggcgaagctg cccgaaaccg
     2821 ggccgatggc cgccatgctg aagaagctgt tcggcgacga ggaatgacca agccggcctt
     2881 gaagggccgg cattcaccgt tcgggcttga cgatcgtcgg cgctatcgcc catagcctga
     2941 cccgaaacat ccctttccgt taggatcaga aaacatgggt ttcaaatgcg gcatcgttgg
     3001 gctgcccaac gtcggcaagt ccacgctgtt caatgcgctg accaggacgg cagcggccca
     3061 ggccgccaac tatcccttct gcaccatcga accgaacacc ggcgaggtgg cggtgccgga
     3121 cccgcgcctg aagaaactgg ccgaaatcgg caagtcgaag gaaataatcc cgacccgcat
     3181 ttcctttgtc gacatcgccg gcctggtgcg cggcgcttcc aagggcgaag gcctcggcaa
     3241 ccagttcctc gccaatatcc gcgaggtcga cgccatcgtc catgtgctgc gctgcttcga
     3301 ggatgacgac atcacccatg tcgagggccg catcgatccg gtcgccgacg ccgagacagt
     3361 cgagacggaa ttgatgctgg ccgatctcga cagcctggaa cgacgcatcg tgcagttccg
     3421 caagcgcgcc gccagcaagg acaaagaagc gctgaccatc ctgccggtca tggagggcac
     3481 gctcgcttta ttgaacgagg gcaagcccgc ccgcgtcatg ttgaagaaca tcgcgccgga
     3541 agatctcgaa atcctgcgca gcctgaacct tctgacctcg caccctgttc tctatgtctg
     3601 caatgtttcg gaggccgacg cggccaccgg caacgagcac acgcgcgccg tggagaagat
     3661 ggcggatgcg cagggcgccg gcaccgtcgt catctcggcc gccatcgagg ccgaggtggc
     3721 gcagcttccc gacgaggaag cacaggaatt cctggccgac ctcgggcttg aagaaccagg
     3781 cctcgaccgc ctcatccgtg ccggctatgc gctgcttcat ctcatcacct acttcaccgt
     3841 cggccccaag gaaacgcgcg cctggaccat tgagcgcggc accaaggcgc cgcaagcggc
     3901 gggcgtcatc cacacggatt tcgagcgcgg cttcatccgc gcgcagacca tagcctatgc
     3961 cgatttcgtg gcgcttggcg gcgaagtcgc ggccaaggaa gccggcaagg cgcgcgacga
     4021 aggcaaggaa tatgtcgtgc aggacggcga cgtgctgctg ttccgcttca acacctgacc
     4081 ggtttcgcct catttccctg gcctaagaca gccggatcgc tcctgcttgc acggcatggc
     4141 ctgccgggtc atggtggcgg tgaaacgtga atgggattgc cagcatgacg gccaggaaat
     4201 gggccttcga ggacttcatc gaaggggcga cgctcgacct cggcagcaag catgtgacgg
     4261 cggaggaaat cgtcgaattc gcctccgaat tcgacgcgca gccgatgcat ctgagcgagg
     4321 aaggcggacg ccagagcatt ctgggcggcc tttcggcttc gggctggcac acctgcgcca
     4381 tgttcatgcg cctgctgtgc gacgccatcc tgctcgattc gacctcgcag ggcgcgccgg
     4441 gcatcgagca ggtcaaatgg aagaagccgg tgttggccgg cgacacgctg accggccgca
     4501 gcacggtgct gggcaagcgg ctgtcgaagt cgcggcctgg tctcggcttc gtctccatgc
     4561 gcgccgaact tttcaaccag cgcggcaaaa gcgtgttcga actcgaaaac gtcggcatgt
     4621 tcctgagccg cgaggccgtc ccgtcatgaa cctcgacgac tatttcgcca tcggcgtcac
     4681 gaccgatctt ggctcgcaca agttcgagcc ggaagagatc aaggtcttcg cccgcaaata
     4741 cgatccgcaa cccttccata ccgacgagat cgcagccagg gacagcgtgt tcggcggcct
     4801 gtgcgcgtcg ggctggcaca ccacggccgt ctggatgaaa cgcaatctgg cgaccggcat
     4861 ggacgggcgg tggaccggcc ccggccccaa gccggaattc ggcccttcgc ccggcttcaa
     4921 gaacctgaaa tggctgaagc cggtctatgc cggcgaaacg atccgcttta cccgcgcctg
     4981 cctcggccac cgcccgcttg cgtcacggcc cggctggaga gtgcttgcca tccgcgcgga
     5041 agcattcgat tccaccggcg acaaggtcat cgagttcgaa aatgccgtgt tggtgaaaac
     5101 ggggtaacgc acgggttctt ccaccgaagc cgccgcgcat aaggcggaaa aaaaccgttc
     5161 ggcttagcta ggcgccactt cagtgccctt cgaaaccgat aagcgttcgc accggcacgc
     5221 ccagcgcctc cagcttcttg cggccgccga gatcgggcag gtcgatgacg aaacaggcgg
     5281 cgatgatgtc cgccccgatc tggcgcagca gcttgaccgc ggcttccgcc gttccgcccg
     5341 tggcgataag atcgtcgatg agaataacct tctcgcccgg cgccacgccg tccttgtgca
     5401 tctccatctc gtccagccca tattcgaggc tgtaggcgac acgcaccgtc tcatgcggca
     5461 gcttgccctt cttgcggatg ggcacaaagc cggcggagag ctgatgcgcc actgcaccgc
     5521 ccagaatgaa gccccgcgcc tcgatgccgg ccaccttgtt caccttctgc ccggcgaagg
     5581 gatgcaccag ttcgtcgacc gcccggcgaa aggccgaagc attgccaagc agcgtggtga
     5641 tgtctcgaaa cagcacgccc ggcttcgggt aatccgggat ggtacggatc gcggcaagca
     5701 acgtggcttc aagggaagaa ttcatgagcg ggattccgtt gacattcagt tctgacgcct
     5761 ggcggcgcaa aagcaaaggg cgcctgtcgg cgccctcgca tttttcctgg ccggcacgga
     5821 ttagtgcgcc acgtcggccc acaccttgcg cttggtcgca tacatcaggc cggcgaacaa
     5881 aagcaggaag atgacgacgc ggaagcccgt cttcttgcgg tcttctagat gcggctcggc
     5941 agcccacatc aggaaggccg aaacgtcctg cgaatactgt tccaccgtct gcggcgagcc
     6001 gtcgccatag gtcacctggc catcggaaag cggcttcggc atcttcagcg acacgcccga
     6061 catgaagtac gggttgtagt gcgtgccctc ggggatcacc ataccggccg gcggctgctg
     6121 atcatagccg ttcagcagcg aatggatata gtccgggccg cccgccgcat actgcgtgaa
     6181 aatatcgatg acgaattgcg ggaagccgcg ctcgacgccg cgcgccttgg caagcagcga
     6241 catatccggc ggagcagcac cgccattggc cgcggctgcc gcctg
//
LOCUS       DASYCS010000373        10554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1702363,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000373 DASYCS010000000
VERSION     DASYCS010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 10554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10554
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1702363"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..990)
                     /locus_tag="VGM46_11620"
     CDS             complement(<1..990)
                     /locus_tag="VGM46_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006326032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEY4193284.1"
                     /translation="MGGALEGTIVVALEQAVAAPLATSRLADAGARVIKLERPEGDFA
                     RFYDDFVLGQSSYFVWNNRGKESCTVDLKQPEDIALVEEMIAGADVFVQNLAPGATDR
                     LGIGSAALRKRHSRLTVCDIIGFAAGTPDEERKAYDLLIQAETGLSGVTGTDLSGPTR
                     VGISICDISTGQAAYAAILQALLLRGKTGQGSHIQISLFDTLAEYLNVPYLGRRYGGR
                     EPKRIGLAHPSIAPYGVFSASDGDVLIAIQNEREWKLFCEHVLANPGLAVDPRFDRNT
                     ARVDNRTELDRTVQAAVARRSVDGLCRLLDKVRIAYGRISTMADLMAHQSATTA"
     gene            complement(1114..2070)
                     /locus_tag="VGM46_11625"
     CDS             complement(1114..2070)
                     /locus_tag="VGM46_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY4193285.1"
                     /translation="MDLRQLRYFTQVVESGSFSKAANILHVAQPALSQHVRHMEEELG
                     VALLYRNAQGVTATEAGERLVRHAKQILAEFAEIPDSVRGEAVAPRGDVRFGMPGTVS
                     ELLAAPLIEAARERYPQVRIRVVEAMSGYILGWLKRGDVDLAMLYSTSDPSGLAVHHG
                     LSEEIFLFAASATGGGPQGDAASMEETVRLPLVIPGPGHGLRELIEQAALTASVPLTP
                     AIEIDSYSQIKKVVMRGLGFGILPLMAISREVEEGVFRMWRLRDPGISRKVYLAYSTE
                     RPLLNAPRAIGQLSWEILRQLVKDRAWIAELSDESRNPDLFR"
     gene            2286..3116
                     /locus_tag="VGM46_11630"
     CDS             2286..3116
                     /locus_tag="VGM46_11630"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEY4193286.1"
                     /translation="MNGDLNDSNDPAETRRYRTMFLSDVHLGTKGSQVGALLEFLRDH
                     DADTIYLIGDIVDGWRLRSGWYWPQSHNDVVQKLLRKARKGSRVVYVPGNHDEFLREF
                     YGTHFGGIEVCETALHEAADGKRYLVIHGDVFDVVVRHARWLAFLGDWAYVAALSLSM
                     RINAVRRSLGFTYWSLSAWAKLKVKNAVNFIGRFEETLTAEAKRQGVDGVICGHIHSA
                     AIREMNGLTYINTGDWVESCTAVVEHEDGRMELIHWAPQSRVLNVATPQLAPWRRNAA
                     "
     gene            3118..4164
                     /locus_tag="VGM46_11635"
     CDS             3118..4164
                     /locus_tag="VGM46_11635"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEY4193287.1"
                     /translation="MDKRVLIVTDAWHPQVNGVVRTLAKLAEHTGAQGAELIFVTPGD
                     FRTVALPFSIDIRLALASKRQISRRIAEIAPDSVHIATEGPLGWQARGVCLAENIAFT
                     TSYHTKFPEYVSARLPVPESWLYGRLRDFHNSGQGMMVATPSLEADLARRGFSHIRRW
                     TRGVDADFFSPDRRTEMDLPRPIFLCVGRVAVEKNLPAFLDLDLPGSKIIVGDGPELA
                     GLKARYPDAHFLGARSNDDLAGIYASSDVFVFPSRTDTFGNVLLEAMASGCPTAAFPV
                     TGPIDVVGSGGVVAEDLREAALAALEVPREAARARAMEYSWPECARQFLSHLRPARGT
                     HVPQGEKAAYVVTS"
     gene            4148..5440
                     /locus_tag="VGM46_11640"
     CDS             4148..5440
                     /locus_tag="VGM46_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008193916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3419 family protein"
                     /protein_id="HEY4193288.1"
                     /translation="MSLPAELPRAAEPSARKHAAKRLRGAVRRNPVFSRKGLSERLFA
                     HLFNGLVYPQIWEDPEVDMEALAIKPGHAIVTIASGGCNALSYLIADPARIEAVDLSP
                     AHVAFNRLKLAALANLPDYEAFYRFYGNADEKENIKAYDRFVRPHLDAESRDYWEKRK
                     LSGRRRISIFSRRLYRHGMLGVFIGMGHYAARIYGIDPRRLLEAGTIEDQSRFFHEEI
                     SPLFDKKAIRWAIRRKSSLFGLGIPPQQYDALVGETGGDMAAVLRKRLEKLACGFPLA
                     DNYFAWQAFGRGYSPTDGGPLPPYLARANFEAVRARAGRLSVVNASYTEFLAAKPAAS
                     TDRFILLDAQDWMSDGQLNDLWREITRTATPGARVIFRTAGEESILPGRIDEAVLGCW
                     TYRAEQSRALHERDRSSIYGGFHLYVLGEDAAQGGQPA"
     gene            5437..6090
                     /locus_tag="VGM46_11645"
     CDS             5437..6090
                     /locus_tag="VGM46_11645"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY4193289.1"
                     /translation="MSATVEHGRLMDSVYRHQRHVYDATRKYYLLGRDPMIEGLRPGD
                     GANVLEIGCGTGRNLIKAARLYPQAKFHGVDISSEMLATAAKSVSASGLQGRIRLAYA
                     DAASFDPAKAFQRRDFDRIFISYAVSMIPAWRQIMQEAMAHLSPGGELHIVDFGDQSG
                     LPKLFKSALYKYLSWYHVTPRASLFETSAGIAAGGDAVTEEKRLYRGFAWISIIRRS"
     gene            complement(6104..6850)
                     /gene="phnF"
                     /locus_tag="VGM46_11650"
     CDS             complement(6104..6850)
                     /gene="phnF"
                     /locus_tag="VGM46_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010970710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HEY4193290.1"
                     /translation="MNQKTRLQRGVGVALWRQIADGIREGIGRGLADEAGRLLPEAQL
                     AERFGVNRHTVRAAIAALAHEGVLQSRQGSGTFVRRQKRLIYPIGERTRFSAGIGGQA
                     RETRSELLGSDQEPAQEPVAAALGVNAGHPLLRIDSLGKADGVPVSRSTAWFDAQRFP
                     LLRDAYAESGSMTEALAKCGVGDYRRASTGVEACHADEADRLELKLSPGAIVIVTRAL
                     NVDLAGVPFQYSVTRFPADRVELVVESGRL"
     gene            6962..7420
                     /gene="phnG"
                     /locus_tag="VGM46_11655"
     CDS             6962..7420
                     /gene="phnG"
                     /locus_tag="VGM46_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HEY4193291.1"
                     /translation="MQPQNANAARKARMSVLSRCDTARLRQLWDGLGLNPPHKTLRGP
                     ESGMVMLRGRVGGQSEVFNLGEATVTRASVRLEDGSVGHAMALGRDAKKALLSALIDA
                     VCHDARMAGHVDAAVIEPMRRELDEADETRRRQTAATRVDFFTLVRGEDA"
     gene            7417..8028
                     /gene="phnH"
                     /locus_tag="VGM46_11660"
     CDS             7417..8028
                     /gene="phnH"
                     /locus_tag="VGM46_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009755715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HEY4193292.1"
                     /translation="MIAASATLEGGFADTVMDAQAAFRAVMNAMANPATVVAVPAQAT
                     PPAPLTQLAGSLACTLMDADTPVWLDGGLALHEAVRAWLGFHTGAPVAASPPDAIFAL
                     VADPSNMPALADFAQGTQEYPDRSATLVLALESLDGGAPLTFRGPGIDGEATFAPLGL
                     PGDFADQWALNAARFPCGVDLILICGDRLACLPRSSRLVREGA"
     gene            8032..9135
                     /locus_tag="VGM46_11665"
     CDS             8032..9135
                     /locus_tag="VGM46_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012759692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HEY4193293.1"
                     /translation="MYVAVKGGEAAIDNAHLLLADRRRGDRSVPALTLDQIVGQMALG
                     VDRVMSEGSLYDRRLAALAIKQARGDLIEAIFLVRAYRTTLPRFGFSEPVDTASMLVE
                     RRISACYKDLPGGQLLGPTFDYTHRLLDEELDGDPAVEPGHVRAGAHEPVTAIADILG
                     GEGLIEDAQVGADAAPGDINQTPMEFPLSRAMRLQSLSRGDEGFLLALGYSTQRGYAR
                     SHPFAGEIRIGEIEVEITVPELDFAVPVGRVQLTECQMVNQFKGSSTAPPQFTRGYGL
                     VFGQSERKAMAMALCDRALRAEELGEDVVAPAQDQEFVISHCDNVQATGFVEHLKLPH
                     YVDFQAELGLVRTMRAEYAERRQGLGDIQEAAE"
     gene            9132..10091
                     /locus_tag="VGM46_11670"
     CDS             9132..10091
                     /locus_tag="VGM46_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018328457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HEY4193294.1"
                     /translation="MNAPSYNFAYLDEQTKRMIRRAILKAIAVPGYQVPFASREMPMP
                     YGWGTGGVQVTAAILGPDDVLKVIDQGADDTTNAVSIRKFFQRVAGVETTTRTGEATI
                     IQTRHRIPETPLRPGQVLVYQVPIPEPLRFLEPRETETRKMHALEEYGLMHVKLYEDI
                     ARNGHIATTYAYPVKVEGRYVMDPSPTPKFDNPKMHMSPALQLFGAGREKRIYALPPH
                     TQVVSLDFEDHPFEVQKFDRPCALCGAEHVYLDEVVLDDSGGRMFVCSDTDHCETRRA
                     SGLHGALSGEILAEEALARQVRAGEAHAGEVLAGKTTSTETGQ"
     gene            10115..>10554
                     /locus_tag="VGM46_11675"
     CDS             10115..>10554
                     /locus_tag="VGM46_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018688558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY4193295.1"
                     /translation="MTEAGVEALPLLSVRSLTKNYGARAGCTDVSLDIWPGEVLAVVG
                     ESGSGKTTLLNCVSTRLEPTAGSICYRMRDGELRDLTRLGEAERRHLLRTDWGFVHQN
                     PADGLRMTVSAGANVGERLMAIGARHYGNIRATATDWLGRVEIDA"
BASE COUNT         1764 a         3534 c         3480 g         1776 t
ORIGIN      
        1 ggcggtcgtg gcgctttgat gcgccatcag gtcggccatg gtggagatgc gcccataggc
       61 gatgcgcacc ttgtcgagca gccggcacag accgtcgacg ctgcggcgcg ccacggcggc
      121 ctgcacggtg cggtcaagct cggtccggtt atcgacgcgc gcggtgttgc ggtcgaagcg
      181 cggatcgacg gcgaggccgg ggttcgccag cacatgctcg caaaacagtt tccactcgcg
      241 ttcgttctgg atggcgatca gcacgtcgcc gtcgctggcg ctgaacacgc cgtagggcgc
      301 gatggaaggg tgggcaaggc cgatgcgctt cggttcgcgc ccgccatagc ggcggccgag
      361 atagggcacg ttcagatatt cggcgagcgt gtcgaacagc gatatctgga tatgcgagcc
      421 ctggccggtc tttccgcgca gcagaagggc ttgcaggatg gcggcatagg cggcctggcc
      481 ggtggaaatg tcgcagatcg agatgccgac gcgcgtcggg ccggaaagat ccgtgccggt
      541 cacgccggaa agcccggtct cggcctggat caggaggtca taggccttgc gttcctcgtc
      601 gggcgtgccg gcggcgaagc cgatgatgtc gcacacggtc agccggctgt ggcgcttgcg
      661 caatgcggcg ctgccgatgc cgagcctgtc ggtcgcgccg ggcgccaggt tctgcacgaa
      721 cacgtcggcg ccggcgatca tctcttccac cagcgctatg tcctcgggct gcttcagatc
      781 gacggtgcag gattccttgc cgcggttgtt ccagacaaaa taggacgact ggccgagcac
      841 aaaatcgtcg tagaaacggg cgaaatcgcc ttccgggcgc tccagtttga tgacgcgcgc
      901 gcccgcgtcg gccagccgcg acgtggcaag cggcgcggcc acggcctgct ccagcgcgac
      961 aacgatcgtt ccctccagcg cgccgcccat cagccgtcct tgccttttcc aagatgcggc
     1021 tcgcactctc gctgaggggg gtgcgaagtc aaatatgaat aaggtatggc gggtatagcg
     1081 ttttgtgcgg tgcggccgcg ctcggcgcgc cggtcagcgg aacaggtctg ggttgcggct
     1141 ttcgtcggag agttcggcga tccaggcgcg gtccttcacc agctggcgca ggatttccca
     1201 agacagctgg ccgatggcgc gcggcgcgtt gagcagcggg cgttcggtcg aataagccag
     1261 atagaccttg cggctgatgc cggggtcgcg caggcgccac atgcggaaga cgccctcctc
     1321 cacctcgcgg cttatggcca tcagcggcag gatgccgaag ccgaggccgc gcatcaccac
     1381 cttcttgatc tggctgtagg aatcgatctc gatggcgggg gtcagcggca cgctcgccgt
     1441 cagagccgcc tgctcgatga gctcgcgaag gccgtggccc ggccccggta tcaccagcgg
     1501 caggcgcacc gtttcctcca tgctggccgc atcgccttgc ggcccgccgc ccgtggcgct
     1561 ggcggcaaac aggaaaatct cctccgacag gccgtgatgc acggccagcc cgctcgggtc
     1621 agaggtcgag tagagcatgg cgaggtcgac gtcgccgcgc ttcagccagc ccaatatata
     1681 gccgctcatc gcctcgacga cgcggatgcg gacctgcgga taacgttcgc gcgccgcctc
     1741 gatcagcggc gcggccagca gttcgctgac ggtgccgggc atgccgaagc gcacgtcgcc
     1801 gcgcggtgcc accgcctcgc cgcgcacgct gtcgggaatt tcggcgaact cggccaatat
     1861 ctgcttggca tggcgcacca gccgttcgcc ggcttccgtc gccgtcacgc cctgcgcgtt
     1921 gcggtagagc agcgctacgc ccaactcctc ctccatgtgg cggacatgct ggctgagcgc
     1981 cggctgcgcc acatgcagta tgttggctgc cttggaaaaa gagccgcttt cgacgacttg
     2041 cgtgaaatat ctcagctgcc gcagatccat ggttgctccg ttcctgaagc ccatcgcgcg
     2101 ggtttcccgt tcggatggcg tcggcacaac ccggatacgt caaagaaggc gcgcctgcta
     2161 ccaccaaaag gatatgagcg gcatattccg tttgcgccag ccggatacgc cctgccctac
     2221 aggcgcggac gggatttcgc cgcccgcgtc actcgcctgt cacggaaatc ggcgaaacag
     2281 cgctcatgaa cggtgatctg aacgattcga acgaccctgc cgagacccgc cggtatcgaa
     2341 ccatgttcct gtcggacgtg catctgggca cgaagggcag ccaggtcggc gcgctgttgg
     2401 aattcctgcg cgaccatgac gctgacacga tctatctcat cggcgatatc gtcgatggct
     2461 ggcggctgcg gtccggctgg tactggccgc aatcgcacaa cgacgtggtg cagaaactgc
     2521 tgcgcaaggc gcgcaagggt tcacgcgtcg tttatgtgcc gggcaaccat gacgagttcc
     2581 tgcgcgagtt ctacggcacg catttcggcg gcatcgaggt gtgcgagacc gccctgcacg
     2641 aagccgccga cggcaagcgc tatctcgtca tccatggcga cgtcttcgac gtggtggtgc
     2701 gccacgcgcg ctggctggcg ttcctcggcg actgggccta tgtggcggcg ctgtcgcttt
     2761 ccatgcgcat caacgccgtg cggcgctcgc tcggcttcac ctattggtcg ctgtcggcct
     2821 gggccaagct caaggtcaag aacgcggtca acttcatcgg ccgtttcgag gaaacgctga
     2881 cggcggaagc gaagcggcag ggcgtcgacg gcgtcatctg cggccacatc cactccgccg
     2941 ccatccgcga gatgaacggg cttacctaca tcaacaccgg cgactgggtg gaaagctgca
     3001 ccgccgtcgt cgaacatgag gacgggcgga tggaactgat ccactgggcg ccgcaaagcc
     3061 gcgtcctcaa tgtcgccacg ccgcaactgg cgccgtggcg gcgcaacgcg gcctgacatg
     3121 gacaagcgcg tcctcatcgt caccgacgcc tggcatccgc aggtcaacgg cgtcgtccgc
     3181 acgctggcca agcttgccga acacaccggc gcgcagggcg ccgagctgat cttcgtcacg
     3241 ccgggcgatt tccgcaccgt cgccctgccc ttttccatcg atatccggct ggcgctggct
     3301 tccaagcggc aaatctcgcg gcgcatcgcc gaaattgcgc cagacagcgt gcatatcgcc
     3361 accgaaggtc cgctcggctg gcaggcgcgc ggcgtgtgcc tggccgaaaa catcgccttc
     3421 accaccagct accacaccaa gttccccgaa tatgtcagcg cgcgcctgcc ggtgccggaa
     3481 agctggctct atggccgcct gcgcgatttc cacaattccg gtcagggcat gatggtggcg
     3541 acgccttcgc tggaggccga cctcgcccgg cgcggcttca gccatatccg gcgctggacg
     3601 cgcggcgtcg acgccgattt cttcagcccc gaccgccgta cggaaatgga cctgccgcgc
     3661 ccgatcttcc tgtgcgtcgg ccgcgtggct gtggaaaaga acctgccggc cttcctcgac
     3721 ctcgacctac cgggcagcaa gatcatcgtc ggcgacggcc cggagcttgc cgggctgaag
     3781 gcgcgctatc ccgatgcgca tttcctcggc gcgcgctcca acgacgatct ggccggcatc
     3841 tacgcttctt cggacgtgtt cgttttcccc agccgcaccg atacgttcgg caacgtcctg
     3901 ctggaagcga tggccagcgg ctgcccgacg gccgcctttc cggtgaccgg gccgatcgat
     3961 gtcgtcggca gcggcggcgt cgtggcggag gatttgcgcg aggcggcgct ggcggcgctc
     4021 gaagtcccgc gcgaggcggc gcgcgcgcgg gccatggaat atagctggcc ggaatgcgcg
     4081 cgccagttcc tcagccattt gcggccggcg cgcggcacgc atgttccaca aggagagaag
     4141 gcagcttatg tcgttaccag ctgaactgcc gcgcgcggct gagccctcgg ccaggaagca
     4201 cgcggccaag cgcctgcgcg gcgcggtgcg ccgaaacccg gtattttcgc gcaagggcct
     4261 ttcggaacgg ctgtttgcgc atctgttcaa cgggctggtc tatccgcaaa tctgggaaga
     4321 cccggaagtg gatatggagg cgctggcgat aaagcccggc cacgccatcg tcaccatcgc
     4381 gtcgggcggc tgcaacgcgc tgtcctacct gattgccgat ccggcccgca tcgaggccgt
     4441 cgatctcagc cccgcccatg tcgccttcaa ccggctgaag ctggccgcac tcgccaacct
     4501 gcccgactac gaggccttct accgattcta cggcaatgcc gacgaaaaag agaacattaa
     4561 ggcctatgat cggttcgttc gtccgcatct cgatgcggaa agccgcgatt attgggaaaa
     4621 gcgcaagctt tccggccggc gccgcatctc catcttttcg cgccggctct accgtcacgg
     4681 catgctgggc gtgttcatcg gcatgggcca ttacgcggcg cgcatctatg gcatcgaccc
     4741 gcgccggctg ctggaagccg gcaccatcga ggaccagagc cgctttttcc acgaagaaat
     4801 ctcgccgctg ttcgacaaga aggcgatccg ctgggcgata aggcgcaaat cctcgctgtt
     4861 cggcctcggc atcccgcccc agcaatatga cgcgctggtc ggcgaaaccg gcggcgacat
     4921 ggccgccgtg ctgcgcaagc ggctggaaaa gctcgcctgc ggctttccgc tggccgataa
     4981 ttacttcgcc tggcaagcct ttgggcgcgg ctacagcccg accgatggcg ggccgctgcc
     5041 accctatctg gcgcgcgcca atttcgaggc ggtgcgcgcg cgcgccggcc gcttgagtgt
     5101 cgtcaacgca tcctacaccg aattcctggc ggccaagccc gccgcctcca ccgaccgctt
     5161 catcctgctc gacgcgcagg actggatgtc ggacgggcag ttgaacgatc tctggcgcga
     5221 gattacccgc accgccaccc ccggcgcgcg cgtcattttc cgcacggcgg gcgaggaaag
     5281 catcctgccc ggccgcatcg acgaagcggt gctcggctgc tggacctacc gggcggaaca
     5341 gtcgcgggcg ctgcatgaac gcgaccgctc gtccatctat ggcggcttcc acctctacgt
     5401 gctgggcgaa gacgccgcgc aaggcgggca gccggcatga gcgcgacggt ggaacacggc
     5461 cgcctgatgg acagcgtcta ccgccaccag cgccatgtct atgacgcgac gcggaaatat
     5521 tacctgcttg gccgcgatcc gatgatcgag ggcctgcgcc ccggcgatgg cgccaacgtg
     5581 ctggagatcg gctgcggcac gggccgcaac ctcatcaaag ccgcgcggct ttatccgcaa
     5641 gcgaagttcc acggcgtcga catctcatcg gaaatgctcg cgactgcggc gaaatccgtg
     5701 tccgcctccg ggcttcaggg ccgtatccgc cttgcctatg ccgacgccgc cagcttcgat
     5761 ccggccaagg cgttccagcg aagggatttc gaccgcatct tcatttccta tgcggtgtca
     5821 atgatcccgg cctggcggca gatcatgcag gaggcgatgg cgcatctgtc gccgggcggc
     5881 gaactgcaca tcgtcgattt cggcgaccag agcgggctgc cgaaactgtt caaaagcgcg
     5941 ctctacaaat atctctcctg gtatcacgtc accccgcgcg ccagcctgtt cgagaccagc
     6001 gccggcatcg ccgccggcgg cgacgcggtg acggaggaaa agcggctcta tcgcggcttc
     6061 gcctggattt cgatcatccg ccggtcgtaa aggcgggatt gtctcacagg cggccggatt
     6121 cgacgaccaa ctcgacgcgg tcggcaggaa agcgcgttac cgaatattgg aacggaacgc
     6181 cggcaagatc gacgttgagc gctcgcgtca cgatgacgat ggcgccgggc gacagtttca
     6241 gttccagcct gtcggcctcg tcggcatggc aggcctcgac gccggtcgat gcccggcgat
     6301 agtcgccgac gccgcatttc gccagcgcct ccgtcatcga tccgctttcg gcataggcat
     6361 cgcgcagcag cggaaagcgc tgcgcgtcga accatgcggt cgagcgcgag acgggaacgc
     6421 cgtcggcctt gccgaggctg tcgatgcgca gcaatgggtg gcccgcattg acgccaaggg
     6481 cggccgcgac cggctcctgc gccggttcct ggtcggagcc aagcagttcg ctgcgcgttt
     6541 cgcgcgcctg cccgccgatg ccggccgaaa accgcgtccg ctcgccgatc gggtagatca
     6601 accgcttctg gcggcgcacg aaggtgccgc tgccctgcct cgattgaaga acgccctcat
     6661 gcgccagcgc cgcaatcgcc gcgcgcaccg tatggcggtt gacgccgaag cgctcggcca
     6721 gctgcgcctc gggcaaaagc ctgcctgcct cgtcggccag cccccggccg atgccttcgc
     6781 ggatgccgtc ggcgatctgg cgccagagcg cgacgccgac cccgcgctgg aggcgtgtct
     6841 tctgattcat ggccattggt ccccgcccgt catccagccg tcatacagat gctctaggga
     6901 ttggatagct tattggattg tctagatcaa tagacaaatt gagaagaaca tggttcctga
     6961 aatgcagcct caaaatgcga atgccgcgcg caaggcccgc atgtcggtgc tgtcgcgctg
     7021 cgacaccgcg cgcctgcgcc agctttggga tggccttggc ctgaacccgc cgcacaagac
     7081 ccttcgcggc ccggaatcgg ggatggtgat gctgcgcggc cgcgtcggcg gacaaagcga
     7141 ggttttcaac cttggcgaag ccaccgtcac ccgcgccagc gtcaggctgg aggatgggtc
     7201 ggtcggccat gcgatggcgc ttggccgcga tgcgaaaaag gcgctgctct cggcgctgat
     7261 cgatgccgtc tgccacgatg cgcggatggc cgggcatgtc gatgccgccg tcatcgagcc
     7321 gatgcggcgc gagctggacg aggccgacga gacgcgccgg cggcagaccg ccgccacccg
     7381 cgtcgatttc ttcaccctgg tgcggggaga agacgcatga tcgccgcttc cgcaacgctg
     7441 gaaggcggct tcgccgacac cgtcatggac gcgcaggcgg ccttccgcgc cgtcatgaac
     7501 gccatggcga acccggcgac ggtcgttgcc gtgccggcgc aggcaacgcc gcccgcgccg
     7561 ctgacgcaac tggccggatc gctggcctgc acgctgatgg atgccgacac gccggtctgg
     7621 ctggacggcg gcctggcgct gcatgaggcg gtgcgcgcat ggctcggttt tcacaccggc
     7681 gcgcccgttg ccgcttcgcc gccggatgct atcttcgcgc tggtcgccga tccgtcgaac
     7741 atgccggcgc tggccgattt cgcgcaaggc acgcaggaat atcccgaccg ctcggcgacg
     7801 ctggtgctgg cgcttgaaag cctggatggc ggcgcgcccc tcacctttcg cgggccgggc
     7861 atcgatggcg aggcgacgtt cgcgccgctt ggcctgcccg gcgatttcgc cgaccagtgg
     7921 gcgctaaacg ccgcgcgctt cccgtgcggc gtcgatctca tcttgatatg cggcgacagg
     7981 ctggcctgcc tgccgcgttc ttcccgcctt gtgcgcgagg gggcctgaac catgtatgtc
     8041 gcggtaaaag gcggtgaagc cgccatcgac aatgcccatc ttcttctggc cgacaggcgg
     8101 cgcggcgacc gttcggtgcc ggcgctgacg ctcgaccaga tcgtcgggca gatggcgctg
     8161 ggggtcgatc gtgtgatgag cgaaggttcg ctctacgatc gccggctggc ggcccttgcc
     8221 atcaagcagg cgcgcggcga cctgatcgag gcgatcttcc tggtgcgcgc ctatcgcacg
     8281 acgctgccgc gcttcggctt cagcgaaccg gtcgacacgg cgtccatgct ggtcgagcgc
     8341 cgcatttccg cctgctacaa ggatttgccg ggcgggcagc tgcttggccc gaccttcgac
     8401 tacacccacc gcctgctgga cgaggaactg gacggcgatc cggccgtcga accgggccat
     8461 gtcagggccg gcgcgcatga gccggtgacg gcgatcgccg atattctggg cggcgaaggc
     8521 ctgatcgagg atgcgcaggt gggggcggac gcagcgcccg gcgacatcaa ccagacgccg
     8581 atggaatttc cgctctcgcg cgccatgcgg ctgcaaagcc tgtcgcgcgg cgacgagggc
     8641 ttcctgctgg cgctcggcta ttccacgcag cgcggctatg cccgctcgca tccgtttgcc
     8701 ggcgaaattc gcatcggcga aatcgaggtc gagataaccg tgcccgaact cgacttcgcc
     8761 gtaccggtcg ggcgcgtgca actgaccgag tgccagatgg tcaaccagtt caagggttcg
     8821 agcacggcgc cgccgcaatt cacgcgcggc tacgggctgg tgttcggcca gtcggagcgc
     8881 aaggcgatgg ccatggcctt gtgcgaccgc gcgctgcggg ccgaggaact gggcgaggat
     8941 gtcgtggcgc cggcgcagga ccaggaattc gtcatttccc attgcgacaa cgtgcaggcg
     9001 accggcttcg tcgagcatct gaagctgccg cattatgtgg atttccaggc cgaactcggc
     9061 ctcgtgcgca ccatgcgcgc cgaatatgcc gagcggcggc agggccttgg ggacattcag
     9121 gaggccgccg aatgaacgcg ccaagttaca acttcgccta tctcgacgag cagaccaagc
     9181 gcatgatccg ccgcgccatt ctcaaggcga tcgcggtgcc gggctaccag gtgccgttcg
     9241 ccagccgcga aatgccgatg ccttatggct ggggcaccgg cggcgtgcag gtaacggcgg
     9301 cgatactcgg cccggacgac gttttgaagg tcatcgacca gggcgccgac gacaccacca
     9361 acgccgtttc gatccgcaag ttcttccagc gcgtcgccgg cgtcgaaacg acgacgcgca
     9421 ccggcgaggc gacgatcatc cagacccgcc accgcattcc cgaaaccccg ctgcggccgg
     9481 gccaggtgct ggtctatcag gttccgatcc ccgagccgct gcgcttcctc gaaccgcgcg
     9541 agaccgaaac gcgcaagatg cacgcgctcg aagaatacgg gctgatgcac gtcaagcttt
     9601 acgaggacat tgcccgcaac ggccatatcg ccaccaccta tgcctacccg gtgaaggtcg
     9661 agggccgcta tgtgatggac ccgtcgccga cgccgaaatt cgataacccc aagatgcata
     9721 tgtcgccggc gctccagctt ttcggcgcgg gccgtgaaaa gcgcatctac gcgctgccgc
     9781 cgcatacgca ggtcgtcagc ctcgacttcg aggatcatcc gttcgaggtg cagaagttcg
     9841 acaggccgtg cgcgctgtgc ggggcggagc acgtctacct ggacgaggtg gtgctcgacg
     9901 acagcggcgg gcggatgttc gtctgttccg acaccgacca ttgcgagacg cgccgggcct
     9961 caggcctgca tggcgcgctt tccggggaaa tactggccga ggaagccttg gccaggcaag
    10021 tacgggccgg cgaagcgcac gcgggcgaag ttctggccgg caaaaccaca tcgacggaga
    10081 cggggcaatg aacgccgccg catccagcgc agtcatgacg gaggcgggcg tcgaggcgct
    10141 gccgctactg tccgtgcgct cgctgaccaa gaactacggc gcgcgcgccg gctgcaccga
    10201 tgtcagcctc gacatctggc cgggcgaagt gctggcggtg gtgggcgaat ccggttccgg
    10261 caagacgacc ttgctcaact gcgtctcgac ccggctggag cctaccgccg gctcgatctg
    10321 ctaccggatg cgcgacggcg aactgcgcga ccttacccgc ctgggcgagg ccgagcgccg
    10381 ccatctcctg cgcaccgact ggggcttcgt gcaccagaac ccggcggatg gcctgcgcat
    10441 gacggtgtcg gcgggcgcca atgtcggcga gcggctgatg gccatcggcg cgcgtcacta
    10501 cggcaatatc cgcgccacgg ccaccgactg gctcggccgc gtcgagatcg acgc
//
LOCUS       DASYCS010000374         4161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2713958,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000374 DASYCS010000000
VERSION     DASYCS010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2713958"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VGM46_11680"
     CDS             complement(<1..185)
                     /locus_tag="VGM46_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193296.1"
                     /translation="MLARLFVIVGGLLVLALCAALVGPYFVDWTGYRADFEREASVIL
                     GRKVTVQGDATARLLPFP"
     gene            complement(268..1683)
                     /locus_tag="VGM46_11685"
     CDS             complement(268..1683)
                     /locus_tag="VGM46_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY4193297.1"
                     /translation="MALSDLSSVERNEEGISAVLGVLKQRFGERFQTGQSIRDQHAHT
                     TTYIPTQAPDGVVFPETTQEVQEIVRSCAEHRVPVIAFGVGSSLEGHVNAPGGGISLD
                     MARMNRVLCLNPQDLDCTVEPGITREELNRYLRDTGLFFPIDPGANASLGGMAATRAS
                     GTNAVRYGTMRENVLSLTAVMADGELVTTGKRARKSSAGYDLTRLLVGSEGTLGIITG
                     LTLKLHGIPQAISGGICPFPTLQAACNAVIATIQIGIPVARIELVNALQMRAMNNYSH
                     LNYAETPCLFVEFHGTDQGVAEQSELFGEIAQDNGGGPFQWTRVAEERTLLWKARHDA
                     YWASMTLRPGARGLSTDVCVPISRLAECVAETEADIAEMGLVAPIVGHAGDGNFHTLV
                     LMDAENPAEIAQVEKFVARLNLRALRMDGTCTGEHGIGQGKAGFMRAEHGHGVDVMRV
                     IKQALDPLNILNPGKILPSGQ"
     gene            1787..2272
                     /locus_tag="VGM46_11690"
     CDS             1787..2272
                     /locus_tag="VGM46_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEY4193298.1"
                     /translation="MPMPAPLVSRIMDIEKDWIDYNGHLNMAYYNVLFDRGSDEALAA
                     LGMGADYAKERRLTIYTAEVHTCYVQELHLDHKVTVSFQLLDHDDKRLRAYQEIRHAD
                     GWLAATCETLSLHVDMAGPKVAPFPADILAGIEGMRAAHACLPMPERAGRAIGIRRKT
                     A"
     gene            2402..2599
                     /locus_tag="VGM46_11695"
     CDS             2402..2599
                     /locus_tag="VGM46_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015317249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193299.1"
                     /translation="MKIDIKVEAARLAADPTISDYDFWRWLKNLNNEIFHIASANEPI
                     PFTMIRWRAILKQARSRRGEA"
     gene            complement(2613..2861)
                     /locus_tag="VGM46_11700"
     CDS             complement(2613..2861)
                     /locus_tag="VGM46_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193300.1"
                     /translation="MEDNFEKYAVAVIITFGALVVGGLMAAAIGFHDRDSFLLALGAT
                     TIAWIAGHAMIFDMPRLYAVLIGLATLMAIASTLALVF"
     gene            3140..3574
                     /locus_tag="VGM46_11705"
     CDS             3140..3574
                     /locus_tag="VGM46_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193301.1"
                     /translation="MNVQDIINTIANQVNIDPLTTEKVVGTILSVLQHEAEGTSAEAL
                     FDKLPGSLDLAQQYDVMAADAASGGGGLLGSLTSALGGALGEKAGALIKGISQLQASG
                     LSMAEIKDAGAALIEQARAAAGPKAVDQALANVPSLKGQLGL"
     gene            complement(3579..3692)
                     /locus_tag="VGM46_11710"
     CDS             complement(3579..3692)
                     /locus_tag="VGM46_11710"
                     /inference="COORDINATES: protein motif:HMM:NF040323.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase superfamily protein"
                     /protein_id="HEY4193302.1"
                     /translation="MADRNYRYMKPGITIEEWGREMAVIDPFGNRIRFMEK"
     gene            3857..>4161
                     /locus_tag="VGM46_11715"
     CDS             3857..>4161
                     /locus_tag="VGM46_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY4193303.1"
                     /translation="MPGFADDMPFFDEPARQPQSAPAGIAARALAARQGSNAAPDYLK
                     GLNPEQRLAVETTEGPVLVLAGAGTGKTRVLTTRIAHILSLGRARPSEILSVTFTNKA
                     "
BASE COUNT          819 a         1362 c         1246 g          734 t
ORIGIN      
        1 ggaaacggca gcagccgcgc tgtcgcatcg ccctgcacgg tgaccttgcg accgagaatg
       61 acgcttgcct cacgctcgaa atcggcgcgg tagccggtcc agtcgacaaa atacggcccg
      121 accagcgcgg cgcacagcgc cagcacgagc aggcctccga caattacgaa caggcgggcg
      181 agcatcagct ccagtcaatc gtttctggtc gtgcgcactc tacctgcatc gccgtgtcga
      241 taaagaggca atcgttgcgc gcgggcatca ttggccggac ggcaaaatct tgcccggatt
      301 gaggatgttc agcgggtcga gcgcctgctt gatgacgcgc attacgtcga cgccatggcc
      361 atgttcggcg cgcatgaagc cggccttgcc ctggccgatg ccatgttcgc cggtgcaggt
      421 gccgtccatg cgcagcgccc gcaggttgag ccgcgccacg aatttctcca cctgcgcgat
      481 ctcggccgga ttttcggcat ccatcagcac cagagtatgg aaattgccgt cgccggcatg
      541 gccgacgatc ggtgcgacca gccccatttc ggcaatgtcg gcctcggttt ccgccacgca
      601 ttcggcaagc cgcgagatcg gcacgcacac atccgtcgac aggcccctgg cgcccggcct
      661 cagcgtcatc gacgcccaat aggcgtcgtg ccgcgctttc cacaacagcg tgcgctcctc
      721 ggccacgcgc gtccactgga acggcccgcc gccattgtcc tgcgcaatct cgccgaacag
      781 ttccgactgc tcggccacgc cctgatcggt gccatggaac tcgacgaaca ggcacggcgt
      841 ctcggcatag ttcagatgcg agtaattatt catcgcccgc atctgcaagg cattgaccag
      901 ttcgatccgc gctaccggga tgccgatttg gattgtagcg atcaccgcgt tgcaggccgc
      961 ctgtagcgtg ggaaacgggc aaatgccgcc ggagattgcc tgcggaatgc catgcagctt
     1021 cagcgtcagc ccggtgatga tgccgagcgt cccttccgag ccgaccagaa gccgtgtcag
     1081 atcgtagccg gcggaactct tgcgggcgcg cttgccggtc gtcacaagct cgccatcggc
     1141 catcaccgcc gtcagcgata aaacgttctc gcgcatcgtg ccatagcgca ccgcattggt
     1201 gccggatgcc cgcgttgcgg ccatgccgcc cagtgaagcg ttggcgcccg gatcgatcgg
     1261 aaagaaaagt ccggtgtcgc gcagatagcg gttcagttcc tcgcgcgtaa tcccaggctc
     1321 gaccgtgcag tcgagatcct gcggattgag gcaaagcacg cggttcatcc gcgccatgtc
     1381 cagcgaaata ccgccgcccg gcgcattgac gtggccttcc agcgaggaac cgacgccgaa
     1441 agcgatcacc ggcacgcggt gttcggcgca actgcgcacg atttcttgca cttcctgcgt
     1501 cgtttcggga aacaccacgc catccggcgc ctgcgtcggg atataggtgg tggtatgcgc
     1561 gtgctggtcg cgaatcgact gccccgtctg gaaacgttcg ccgaaacgct gcttgaggac
     1621 gccaagcacg gccgaaatgc cttcctcgtt gcgctcgacg ctcgacaggt cgctcagagc
     1681 catgcattcc tccgcaaacg ggcaagtgca attattcttt gcattctatg gatggggtag
     1741 cccctccagt cacgccctgt ccagcacctg ccagaaggaa atttcgatgc ccatgcccgc
     1801 cccgctcgtc tcccgcatca tggacatcga aaaagactgg atcgactata atggccacct
     1861 caacatggcc tattacaatg tgctgttcga ccgcggttcc gacgaagcgc tcgctgccct
     1921 gggcatggga gccgactacg ccaaggagcg tcggctgacc atctatacgg cggaagtcca
     1981 cacctgttac gtgcaggaac ttcacctcga ccacaaggtc accgtcagct tccagctgct
     2041 cgatcatgac gacaagcggc tgcgcgccta ccaggaaatc cgccatgccg atggctggct
     2101 cgccgccacc tgcgaaacgc tgtcgctgca tgtggacatg gcagggccga aggtagcgcc
     2161 ctttccggcc gatattctgg ccgggatcga gggaatgcgc gccgcgcacg cttgcctccc
     2221 catgccggag cgggccggcc gggccatcgg catccgccgc aagaccgcgt gagcctggcc
     2281 gttaacctta ccaactctta aacgccaaca ggcaaacctt cttcattgat cgaatcggcc
     2341 gctcgttgga gactcccaca aatcgcagag ccgcggcggg caaagcgaat gagggacggg
     2401 catgaaaatc gacatcaagg tcgaggcggc caggctggcc gccgatccga ccatttccga
     2461 ctatgatttc tggcgttggc tgaagaacct caacaacgag atttttcaca tcgccagcgc
     2521 caatgagccg atccccttca cgatgatccg ctggcgcgcc atcctgaagc aggcgcgcag
     2581 ccggcgcggc gaggcctgaa ggccagcgct gttcagaaaa ccagcgcgag cgtggaggct
     2641 atggccataa gcgtggcaag gccgataagt accgcataaa ggcgcggcat gtcgaagatc
     2701 atggcgtggc cggcgatcca ggcgatcgtc gtggcgccca gcgccaaaag gaagctgtcg
     2761 cggtcgtgaa agccgattgc cgccgccatc aggccgccaa cgaccagcgc cccgaaggtg
     2821 atgatgaccg cgacggcgta tttttcgaaa ttgtcttcca ttgcgggtcc tttgcgcgcc
     2881 tgattgcatg cggaggtgcg ccgaccggtt tcaggcgctt gcgagtgaca gatttaaacc
     2941 atcggctatc atggacaagg ccaaagaatg gcaatgtcgc cctatccgcc ggtttgcaac
     3001 gcccatcgcc tggtccgcaa cctttttcgg cggcgcaaag gccgatcacg gcgaaatgag
     3061 cggcccgaat cacgctgggc gcttgcaagc gcgacgccgg tctgaatagc gtggatggtc
     3121 gagcacgaaa ggaaccgcca tgaacgtcca ggacatcatc aacaccatcg ccaatcaggt
     3181 caatatcgac ccgctgacga cggaaaaggt cgtgggcacc attttgtccg tgcttcagca
     3241 cgaggcggaa ggcaccagcg ccgaagccct tttcgacaaa ttgccgggct cgctggattt
     3301 ggcccagcaa tatgacgtca tggccgccga cgcggcttcg ggcggcggcg gcttgcttgg
     3361 atcgctgacc tcggcgcttg gcggcgcatt gggcgagaag gccggcgcgc tcatcaaggg
     3421 catttcgcaa ttgcaggcct ccggcctcag catggcggaa atcaaggacg ccggcgcggc
     3481 gctgattgag caggcaaggg ccgcggccgg ccccaaggct gtcgatcagg cgctggccaa
     3541 tgtgccgagc ctgaaaggcc agctcggact atagcgcctc atttctccat gaagcgaatg
     3601 cggttgccga acgggtcgat caccgccatc tcccgccccc attcctcgat ggtgatgccg
     3661 ggcttcatgt agcggtagtt ccggtcggcc atccgtcgga atgcgggcca tcggccgcat
     3721 cgaaccgcac gaacgctatt agcgaggcta ttttcggtca atataccgtc aatatggatg
     3781 cggccgagcc ggaataccgg aacaaaacgg agacgcttct ggcctttccg ttccgaatca
     3841 ctacattcca ctgaagatgc ccggctttgc agacgacatg cccttcttcg acgaacccgc
     3901 gcgccagccc cagtctgcgc cggcgggcat tgccgcgcgc gcgcttgccg ccaggcaggg
     3961 cagcaacgcc gcgcccgact atctgaaggg ccttaacccc gaacagcggc tggcggtgga
     4021 aaccaccgag gggccggttc tggttctggc tggcgccggc accggcaaaa cccgcgtact
     4081 gaccacccgc atcgcccaca tcctgagcct cggccgcgcg cgcccgtcgg aaatcctctc
     4141 ggtcaccttc acgaacaagg c
//
LOCUS       DASYCS010000375         4496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3583810,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000375 DASYCS010000000
VERSION     DASYCS010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4496
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3583810"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..1357
                     /locus_tag="VGM46_11720"
     CDS             101..1357
                     /locus_tag="VGM46_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY4193304.1"
                     /translation="MDQGNEAGEVPPAQYAVRIAPGIGAFTRQEWDSLSGTVRGDSIN
                     GYNPFISFAFLSALEDSGCALRSTGWQGHHLRLETPEGRLLGAVPCYVKSHSQGEYVF
                     DHGWADAFERAGGSYYPKLQCSIPFTPATGPRLLVVSGENADTVKAGLASALKLVTAK
                     LGVSSAHVTFVPEDDAEILEAAGFLHRTDQQFHFFNEDFSTYDDFLATLASRKRKALK
                     KERREALANGITIDRLTGDAITEKVWDDFYAFYTDTGSRKWGRPYLNREFFSLIGERM
                     ADDILLVMAKRDGRYVAGAINFIGSDTLFGRNWGCIEDHPFLHFEVCYHQAIDFAIER
                     KLKVVEAGAQGEHKLARGYRPVTTHSAHYIAHPGLRDAIADYLRRERREVERISDYLE
                     EHTPFRKGDPALNGLPSSDTDRDQEA"
     gene            1443..1865
                     /locus_tag="VGM46_11725"
     CDS             1443..1865
                     /locus_tag="VGM46_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019858217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEY4193305.1"
                     /translation="MAYDDTNIFAKILRGEIPSHKVYEDDAVVAFMDVMPQGQGHTLV
                     VPKAPSRNLLDADPATFGALFSVVQKLAVAVKKAFAADGVTIQQFNEPAAGQTVYHLH
                     VHVLPRFEGIALRPHTGEMEKPEILSENARKIRAALAA"
     gene            complement(1937..2536)
                     /gene="wrbA"
                     /locus_tag="VGM46_11730"
     CDS             complement(1937..2536)
                     /gene="wrbA"
                     /locus_tag="VGM46_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004686441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase type IV"
                     /protein_id="HEY4193306.1"
                     /translation="MAKVLVLYYSSFGHMEQMAKAAAEGARAGGADVTIKRVPELVPE
                     EVAKASHYKLDQQAEIATPAELENYDAIIFGSATRYGAIASQMKNFIDQTGPLWAKGA
                     LLDKVGSVMVSSATQHGGAEIALLATQASLQHHGMIIVPLSYAYQGQMGNDVVRGGSP
                     YGMTTTSDGDGSRQPSAQELEGAHFQGKRVAEIAAKLHG"
     gene            2716..3456
                     /locus_tag="VGM46_11735"
     CDS             2716..3456
                     /locus_tag="VGM46_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEY4193307.1"
                     /translation="MTEIVTAAMLVIGDEILSGRTKDKNIGHLADIMTAVGIDLKEVR
                     VVPDEEDEIVAAVNAMRARYTYVFTTGGIGPTHDDITACSISKAFGVPCEYDAKAYAM
                     LEASYAKRGIEFTEARKRMARMPRGADHIDNPVSLAPGFRIGNVHVMAGVPSIFQAML
                     DNVIPTLKTGTKMLSATVECPFGEGLIGGPLGEIQKTHPDTIIGSYPKYGDGKFWTEL
                     VVRARSQEALDKARADVEAMVAGLAKAG"
     gene            3554..4387
                     /locus_tag="VGM46_11740"
     CDS             3554..4387
                     /locus_tag="VGM46_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY4193308.1"
                     /translation="MRFKTIAAILQSEHDVGRVLDCAVPLAKRFESHLVGIHAEALPI
                     PYTSATGFPDTEFLQVSAAMNKERADKVHAAFLKTIENAGVSFEWRSLESFSGDSALS
                     GISSVRAADLVVAAQRESGGDAKADIDTLVYDAGRPVLVVPHTGAIVTTFKHVLLAWN
                     GTREAARAAFDALPFMLEAEKTEILIIDPPDMVDESADNAGVEIAAALARHGAKVTVS
                     VQKSEGSAVEDIIQNRLSGTGADLLVLGAYSHSWLRRLLFGGVTQSVLRSGQVAAFLS
                     R"
BASE COUNT          868 a         1375 c         1393 g          860 t
ORIGIN      
        1 cagatgacgt tcgagggttt cgagccggat gccgtgcgta ttgcctgatg ttcctcgaca
       61 tcgggcttgc atggacgtgg cgccaactta agtaagtccc atggatcagg gcaacgaagc
      121 aggcgaagta ccaccggcgc aatatgcagt gcgtatcgcg ccgggcatcg gggcctttac
      181 ccggcaggaa tgggacagtc tttcgggaac cgtgcgcgga gattcaataa acggctataa
      241 ccctttcatt tcctttgcgt tcctgagcgc gctggaggat tccggttgcg ccctgcgcag
      301 caccggctgg caaggccatc atttgcggct ggaaacgccg gaaggcaggt tgctgggcgc
      361 cgtgccgtgc tacgtcaaat cgcacagcca gggcgaatat gtgttcgatc atggctgggc
      421 cgatgcgttc gagcgggccg gcggcagcta ctatcccaag ctgcaatgtt ccattccgtt
      481 tacgccggcc accggccctc gcctgctcgt cgtaagcggc gagaatgccg atacggtcaa
      541 agccgggctg gcctccgccc tcaagctggt gacggcaaaa cttggcgttt cctccgccca
      601 tgtcacgttc gtgccggaag acgatgccga gatactggag gccgcaggct tcctgcatcg
      661 caccgaccag caattccatt tcttcaacga agacttttcc acctatgacg atttcctggc
      721 gacgctcgcc tcacgcaagc gcaaggcgct gaaaaaggaa cggcgcgagg cgctggccaa
      781 cgggatcacg atagaccggc tgacgggcga tgcgatcacg gaaaaggtct gggacgattt
      841 ctacgccttt tatacggata ccggcagccg caaatggggc cggccctatc tcaaccgcga
      901 gttcttctcg ctgatcggcg agcgcatggc cgacgacatt ctgctggtga tggccaagcg
      961 cgatggccgc tacgtcgccg gcgcgatcaa cttcatcggc tccgacacgc ttttcgggcg
     1021 caactggggc tgcatcgagg atcacccgtt cctgcatttc gaagtttgct accatcaggc
     1081 catcgacttc gccatcgagc gcaagctgaa ggtcgtcgag gccggcgcgc agggcgagca
     1141 caagctggcg cgcggctatc gcccggtgac cacgcactcc gcgcattata tcgcgcatcc
     1201 gggcctgcgc gacgccatcg ccgattacct gcggcgcgaa cgccgcgaag tcgaacgcat
     1261 tagcgattac ctggaagagc atacgccgtt tcgaaaaggc gatccggcgc tgaacgggct
     1321 gccgtcttcg gatacggatc gcgaccagga agcatagaat gtcgcggcat acccgcctgc
     1381 cgtgctgatt cggcgtcagc cgacgggcat tgcggcaaca caggcagaag cgagataccg
     1441 acatggccta tgacgacacc aacatcttcg ccaagatcct gcgtggagaa attccgtcgc
     1501 acaaagtcta tgaagatgac gcggtggtgg ccttcatgga cgtgatgccg caagggcaag
     1561 gccatacgct ggtggtgccg aaagcgccgt ctcgcaatct gctcgatgcc gatcccgcca
     1621 ctttcggcgc gcttttttcg gtggtgcaga agctggcggt cgcggtgaaa aaggcgtttg
     1681 cggccgatgg cgtcaccatc cagcagttca acgagccggc ggccggccag accgtgtacc
     1741 acctccacgt ccatgttctg cctcgcttcg agggtatcgc attgaggccg catacgggcg
     1801 agatggaaaa gccggaaatc ctttctgaaa acgccaggaa aattcgcgca gcgcttgccg
     1861 cctgaccgct cacatagggc gcgcacaaaa aagggcggcc gatgggccgc cctttttcat
     1921 tcaattgacg aagcaatcag ccgtgcagct tggccgcgat ttcagctacg cgcttgccct
     1981 ggaagtgcgc gccttccagt tcctgcgcgg aaggctggcg cgaaccgtcg ccgtcgctgg
     2041 tggtggtcat gccgtagggc gaaccgccgc gaaccacatc gttacccatc tggccctgat
     2101 aggcataaga cagcggcaca atgatcatgc cgtgatgctg gagcgaggcc tgcgtcgcca
     2161 acagcgcgat ttccgcgccg ccatgctggg tagcggacga aaccatcacc gagccgacct
     2221 tgtcgagcag cgcgcccttg gcccacagcg gaccggtctg gtcgatgaag ttcttcatct
     2281 gcgaggcgat cgcaccgtag cgggtggccg agccgaagat gatggcgtcg taattttcga
     2341 gttcagccgg cgtcgcgatt tcggcctgct ggtcgagctt gtagtgcgaa gccttggcga
     2401 cttcttccgg aaccagttcg ggaacgcgct tgatcgtcac gtcggcgccg ccggcgcgcg
     2461 cgccttcggc cgcagccttg gccatctgct ccatatgccc gaagctcgaa taataaagta
     2521 ccagaacttt tgccattacg ttccccttga tctgcaaaca ggccgacacg gattgcgtcg
     2581 tcggccccta tgtaaagggc gcgccgttca tcgcaaaccc cggatgtggc gacagatcgt
     2641 tcatcattcg gctgccattg cccagcctgc ggcgggggag tatggactgg ccttccctgt
     2701 tgccggagtg ctttcatgac cgagatcgtt accgccgcca tgctggtcat cggcgatgag
     2761 attctgtcgg gccgcaccaa ggacaagaat atcggccacc tggcggatat catgacggcg
     2821 gtgggcatcg acctgaaaga agtccgcgtc gttcccgacg aggaagatga aatcgttgcc
     2881 gcggtgaacg ccatgcgggc gcgctacacc tatgtgttca cgaccggcgg catcggcccg
     2941 acgcatgacg acatcaccgc ctgctccatc tcgaaggcgt tcggcgtgcc gtgcgaatat
     3001 gacgccaagg cctacgccat gcttgaagcg agctatgcca agcgtggcat cgaatttacc
     3061 gaggcgcgca agcgcatggc gaggatgccg cgcggcgccg accatatcga caatcccgta
     3121 tcgctggcgc cgggcttccg catcggcaac gtccacgtca tggccggggt gccgtcgatc
     3181 ttccaggcga tgctcgacaa tgtcattccg accctgaaga caggcacgaa aatgctttcg
     3241 gcgacggtgg aatgtccgtt cggcgagggg ctgatcggcg ggccgctggg cgagatccag
     3301 aagacgcatc ccgacaccat catcggttcc tatccgaaat atggcgacgg taaattctgg
     3361 accgaactgg ttgtgcgcgc ccgctcgcaa gaagcgctgg acaaggcgcg cgccgacgtg
     3421 gaggcgatgg tagccggcct tgccaaggca ggctgacggc taaatcttcg cccttatgcg
     3481 gataattgcg gaaccgtcgc gcagctttcg cgtttcgtcg gtagacctag agtctgcata
     3541 agagggaatt ttcatgcgtt tcaagactat cgccgctatt cttcaaagcg agcacgatgt
     3601 gggccgcgtt ctcgattgcg ccgtgccgct ggccaaacgg ttcgaaagcc atctcgtcgg
     3661 catccatgcg gaggcgttgc cgatccccta tacgtcggcg accggttttc ccgacactga
     3721 attcctccag gtctcggcgg caatgaacaa ggagcgcgcc gacaaggtgc atgctgcctt
     3781 cctgaagacc atcgaaaacg cgggcgtatc gttcgagtgg cgcagcctcg aaagcttttc
     3841 gggcgacagc gcgctttcag gcatttccag tgtccgcgcc gccgacctgg tggtcgcagc
     3901 gcagcgcgag tccggcggcg acgccaaggc cgatatcgac acgctggtct acgatgccgg
     3961 acgaccggtg ctggtggtgc cgcatacggg cgcaatcgtc accaccttca agcatgtgct
     4021 gctggcatgg aacggcacga gggaggcggc gcgcgccgct ttcgatgcgc tgcccttcat
     4081 gcttgaggcc gaaaagaccg aaatcctcat catcgacccg ccggatatgg tggatgaaag
     4141 cgccgataat gccggcgtcg agatcgcggc cgcccttgcc cggcacggcg ccaaggtgac
     4201 cgtatcggtg caaaagtcgg aaggcagcgc ggttgaagat atcatccaga accgcctttc
     4261 cggcaccggc gccgacctgc tggtgcttgg cgcctacagc cattcatggc tgcggcggct
     4321 gttgttcggc ggcgtaacgc agtccgtcct gcgctcgggc caggttgccg ccttcctgtc
     4381 gcgataaggc tggcattggc catggcgtct ccttggcgca agtcggtacg gcggttcctg
     4441 catagggtgc caccgttttg cgccaaggga tgaccgaata tgccgcgacc ggattt
//
LOCUS       DASYCS010000376         7000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3293965,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000376 DASYCS010000000
VERSION     DASYCS010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 7000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7000
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3293965"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VGM46_11745"
     CDS             <1..274
                     /locus_tag="VGM46_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HEY4193309.1"
                     /translation="DSLDAFGVHGVGGIVGALLTGIFADPAINALGANASVATQLYGI
                     AVTIVWTGIATFVILQVVKLLLGGLRPSKQEEIEGLDITQHGEVVP"
     gene            complement(454..1722)
                     /gene="lysA"
                     /locus_tag="VGM46_11750"
     CDS             complement(454..1722)
                     /gene="lysA"
                     /locus_tag="VGM46_11750"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEY4193310.1"
                     /translation="MNHFDYREGVLHAEDVAIPDIAAAVGTPFYCYSTATLTRHFRVF
                     SEAFSELDALVCYALKANSNQAVLKTLAKAGAGADVVSEGELRRALAAGIAARKIVFS
                     GVGKTAQEMDFALEADILCFNVESEPELELLSARAVALGRTARISLRINPDVDAKTHK
                     KISTGKAENKFGIEWRRAREVYGRAAKLPGIKVTGIDTHIGSQITDLQPFDDAFALLV
                     DLAGQLRTDGHDIDHVDLGGGLGIPYRTDNSPPPLPDAYAAIVKKHVGKLGLKVMFEP
                     GRLIVGNAGILVSQVIYVKEGAARNFLIVDAAMNDLVRPTLYDAFHDIRPVVEQEASA
                     PRMVVDVVGQVCETGDYLGLDRDLPRMKAGDLIAVSTAGAYGAVQAGTYNTRLLVPEV
                     LVDGGRFHVVRARPTYDELIGLDSIPDWLE"
     gene            complement(1742..1951)
                     /locus_tag="VGM46_11755"
     CDS             complement(1742..1951)
                     /locus_tag="VGM46_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193311.1"
                     /translation="MIAGRMLVTLTLLAAMATVVGCGRRAGLDTPYEAAVQARKDAEK
                     AKQPLPPEPEKPVADKPFFLDKLIQ"
     gene            complement(2083..3483)
                     /gene="argH"
                     /locus_tag="VGM46_11760"
     CDS             complement(2083..3483)
                     /gene="argH"
                     /locus_tag="VGM46_11760"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEY4193312.1"
                     /translation="MSETKASNQMWGGRFASGPAAIMEAINASIGFDRKLCTQDIRGS
                     IAHSAMLAQTGIISAADQEKIAHGLNTILAEIEAGKFEFSTKLEDIHMNVEARLAELI
                     GPAAGRLHTARSRNDQVAVDFRLWVKDELFRIVSALKGLIAAFLERAQEHAATVMPGF
                     THMQSAQPVTFGHHCMAYVEMFSRDLSRVRDAIERMDESPLGAAALAGTGFPVDRHMT
                     AKALGFREPTRNSLDSVSDRDFALEFLSVAAICATHLSRLAEEIVIWSTPQFGFVRLS
                     DSFSTGSSIMPQKKNPDAAELVRAKTGRVNGHLMALLTVMKGLPLTYSKDLQEDKEAV
                     FDTAETLDLMLAAMTGMVRDLTVNVAAMKKAAGAGYSTATDLADWLVRTLSLPFREAH
                     HITGRAVALAEARKTGLEKLSLQDLQTIHPGITDDVFSVLGVQSSIKSRVSFGGTAPA
                     EVRKQVRYWKKRLAKA"
     gene            3517..4209
                     /locus_tag="VGM46_11765"
     CDS             3517..4209
                     /locus_tag="VGM46_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY4193313.1"
                     /translation="MAEDRKLFPAARLVLAALVAGALAGAVAVYVKESGSGNTPGTQP
                     AQQFAIGESPDDVACAAKSEQAKKIAASATGQVAALLPADPPQSLKDLAFDGPDGKPM
                     TLDNHAGKTVLLNLWATWCAPCRAEMPALDALQKEKGGDKFEVVAVNVDTGDDAKPKK
                     FLGDIGVGKLGYYSDHSMAVFNDVKTRGLALGLPVTMLVDGEGCLIAHMNGPAEWSGG
                     DARRLVEAAIGG"
     gene            4351..5376
                     /locus_tag="VGM46_11770"
     CDS             4351..5376
                     /locus_tag="VGM46_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010660704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY4193314.1"
                     /translation="MAFGWGIVGTGAIARQFAADLTHAPGAHIAAVHSRSPEKAEAFR
                     AAFKAGRAYGDLDALLADGAVDAVYIATPNAAHAEQALLAIAAGKPVLVEKPLALAGA
                     DAGRIEAAALAKGTFAMEAMWTRFLPAVRRARAKVALGEIGPIRAIRANLSYVHDETP
                     GSRFYEPQGGGALYDLGVYPISLALHFLGHPESVSGRWFAAQSGVDKSAEVELHYDRA
                     VARLSCGFDRTGENSLLVEGTTGTLRLRAPFLKAQRLTHYSRADSEIPLLEERGLSKI
                     LSGWLVPGRKVEQLRFPGGGLQFEAIAVMDAVRQGKTQSDLMPLDESRAALAIIEQVL
                     SQPPSAA"
     gene            complement(5373..6548)
                     /locus_tag="VGM46_11775"
     CDS             complement(5373..6548)
                     /locus_tag="VGM46_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011265393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY4193315.1"
                     /translation="MKIAFLGTGFVADYYMASLANYSGDNYSGANRAGLELAGAFDRD
                     AARLAQFCDYHHVRAYDSVEALLADESVGIVVNLTTPESHFETSRRALLAGKHVYCEK
                     PLAMRFEDARILVELADELGLTLATAPANALSAAHSLVAGALDAGRIGTPRLVYAEME
                     DGAVFRDKWTEWRSRSGAKWPGAHEFEIGCTLEHAGYALSWLTSLFGPVESLTAFSAL
                     AFPDKGPGTQGLALAPDFSVGCLAFKSGVVARLTSGLAAPKDRSLTILGDNGSITVRD
                     LWDEHSTIHLETENDRRSLKIRLTNRLESRLGKFLPWKPTVGRRLTYPATSARKALPG
                     FPSQIDFCAGIAAQASAITEKQKPQFSGALALHITELALALNNARNLPQPYRLKSTF"
     gene            6696..>7000
                     /locus_tag="VGM46_11780"
     CDS             6696..>7000
                     /locus_tag="VGM46_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEY4193316.1"
                     /translation="MSFIPDIATLLQFAIATVILAVTPGPDMTLFVSRTLSQGRATGF
                     ASMTGALTGTLVHTTLVVVGISALIVASPMAFFVLKIFGAGYLVFLAWQALAKGSAFT
                     "
BASE COUNT         1271 a         2274 c         2165 g         1290 t
ORIGIN      
        1 cgacagcctc gatgcgttcg gcgtccatgg cgtcggcggc attgtcggcg cgctgctgac
       61 cggcatattc gccgatccgg caatcaacgc gctcggcgcg aacgcctccg tcgccaccca
      121 gctctacggc atcgcggtca ctatcgtgtg gaccggcata gccaccttcg tgatcctcca
      181 ggtggtcaag ctgctgctcg gcggcctgcg ccccagcaag caggaagaga tcgagggtct
      241 cgatatcacc cagcatggcg aggtggtgcc ataagcctca ccgccagggt tgcgccggca
      301 aaatagccgg cgtcgccgat tttccgcgtg tctcccgcca ggagagatga cgccctgagg
      361 cccgacttgt tcgggcctct tttttaccca gacagactta acggcccgga cctgcttcgg
      421 gcctcctttt tatccagatg gatcggaaag cgactattcc agccagtcgg ggatgctgtc
      481 caggccgatc agctcgtcat aggtcggacg cgcgcgcacg acatggaagc ggccgccatc
      541 gaccagcacc tcgggcacca acagcctcgt attataggtg ccggcctgca ccgcgccata
      601 agcgccggcg gtgctgacgg caatcaggtc gcccgccttc atcctcggca aatcccggtc
      661 gaggccgaga tagtcgcccg tctcgcaaac ctggcccacc acatcgacga ccatgcgcgg
      721 cgcggacgcc tcctgctcca cgaccgggcg aatgtcatgg aaggcatcgt agagcgtcgg
      781 gcgtaccaga tcgttcatcg ccgcatcgac gatgaggaag ttcctggccg ccccttcctt
      841 cacatagatc acttgcgaaa ccagaatgcc ggcattgccg acgatcagcc gccccggctc
      901 gaacatcacc ttcaggccaa gcttgccgac atgcttcttg acgatcgccg cataggcgtc
      961 gggcagcggc ggcgggctgt tgtcggtgcg gtaagggatg ccgaggccgc cgcccagatc
     1021 gacatgatcg atgtcgtggc cgtcagtgcg caactgtccg gccaggtcaa ccagcagggc
     1081 aaaagcatcg tcgaaaggct gtagatcggt gatctggctg ccgatatgcg tatcgatgcc
     1141 ggttaccttt atgcccggca gcttggcggc gcggccataa acctcacgcg cgcgccgcca
     1201 ttcgatgccg aacttgttct ccgccttgcc ggtggaaatc ttcttgtgcg tcttggcgtc
     1261 gacgtccggg ttgatgcgca gcgagatgcg cgctgtccgg cccaaagcca cggcgcgcgc
     1321 cgatagcagt tccagttcag gctcggattc gacattgaag caaagaatat ccgcttccag
     1381 cgcgaaatcc atttcctgcg cggtcttgcc gacgccggag aaaacgatct tgcgcgccgc
     1441 aatgccggcg gcaagcgcgc gccgcaactc gccttccgaa acgacatcgg cgccggcgcc
     1501 cgccttggcc agggtcttca gcaccgcctg gtttgaattc gccttcagcg cgtagcagac
     1561 cagcgcgtcc agttcggaaa acgcctccga gaacaccctg aaatggcggg tcagcgtcgc
     1621 cgtcgaatag cagtagaacg gcgtgccaac ggccgccgca atatcgggaa tggccacgtc
     1681 ctcggcatgg agcacgcctt cgcggtagtc gaaatggttc acggggggtt ttccggaaag
     1741 gctactggat gagcttgtcg aggaagaagg gcttgtcggc gaccggcttt tccggctccg
     1801 gcggcagcgg ctgctttgct ttctcggcgt ccttgcgggc ctgcaccgcg gcctcgtaag
     1861 gggtgtcgag cccggcgcgc ctgccgcaac cgacgacggt cgccatcgct gccagaagcg
     1921 taagcgtcac caacatcctg cctgcgatca tcgtttcacc tgcccaaacc acgaaaacgc
     1981 tgtttctaga gcattccagc aaaagtgtga aacggttttg catccggaaa tagggcaaag
     2041 caaagagcta gagccgaatt accggtgctt tgcaccccgc cattatgcct tggccagccg
     2101 cttcttccag taacgcacct gcttgcgcac ttccgccggc gcggtgccac cgaacgaaac
     2161 gcggcttttg atcgagcttt gcacgccaag caccgaaaac acatcgtcgg taatgccggg
     2221 atggatcgtc tgaaggtctt gcagcgacag tttctccagc ccggttttgc gcgcttcggc
     2281 caacgccacc gcgcggccgg taatgtgatg ggcctcgcgg aacggcagcg aaagcgtgcg
     2341 caccagccag tcggccagat cggtggccgt ggaatagccg gcgcccgcgg cctttttcat
     2401 ggcagcgaca ttgaccgtca ggtcgcgcac catgccggtc atcgccgcca gcatcaggtc
     2461 gagcgtttcg gcggtgtcga agacggcttc cttgtcttcc tgcaagtcct tcgaataggt
     2521 cagcggcaac cctttcatga cggtcaaaag cgccatcaga tgaccgttga cgcgaccggt
     2581 cttggcccgc accagttcgg cggcgtccgg gttcttcttc tgcggcatga tcgaggaacc
     2641 ggtcgaaaac gagtccgaca ggcgcacgaa gccgaattgc ggtgtcgacc agatcacgat
     2701 ctcttccgcc agccgcgaca gatgcgtcgc gcaaatcgcc gccaccgaca ggaactccag
     2761 cgcgaaatcg cggtcggaga cgctgtccag cgaattgcgc gtcggctcgc ggaagccgag
     2821 cgccttcgcc gtcatgtgcc gatcgacggg aaagccggtg ccggcgagcg cggcggcgcc
     2881 gagcgggctt tcatccatcc gctctatggc gtcgcgcacg cgggaaagat cgcgcgaaaa
     2941 catctcgaca taggccatgc agtggtggcc gaaagtcacc ggttgcgccg actgcatgtg
     3001 ggtgaagcca ggcatgacgg tcgccgcatg ttcttgcgcc cgctccagga aggccgcgat
     3061 cagccctttc agcgccgaaa cgatgcggaa aagctcgtcc ttgacccaca ggcgaaaatc
     3121 gaccgctacc tggtcgttgc gcgagcgcgc cgtatgcagc cgcccggccg ccggcccgat
     3181 caattcggcc agccgcgcct cgacgttcat gtggatgtct tcgagcttcg tcgagaactc
     3241 gaatttgccg gcttcgattt ctgccagaat cgtgttcagc ccgtgagcga tcttttcttg
     3301 atcggcagcc gaaataatgc ccgtttgcgc cagcatggcg ctatgggcga tcgagccgcg
     3361 aatgtcttgc gtacagagct ttcggtcgaa gccgatggag gcgttgatgg cttccatgat
     3421 cgccgccgga cccgaggcaa aacgtccgcc ccacatctgg ttgctggcct ttgtttcgct
     3481 catcgctata acccgtctta cagagtggtt ttaaaaatgg cagaagacag aaaacttttc
     3541 cccgccgcgc gcctcgtcct tgccgccctt gtggctggcg cactggccgg cgcggtcgcg
     3601 gtatatgtca aggagagcgg ttctggcaac acgccaggca cgcagcctgc ccagcagttc
     3661 gccattggcg agagcccgga cgacgtcgcc tgcgccgcca agagcgaaca ggcgaaaaag
     3721 atcgcggcgt ccgccactgg ccaggtagcg gcgctgctgc ccgccgatcc gccgcaatcg
     3781 ctcaaggatc tcgccttcga cgggccggac ggcaagccga tgacgctcga caaccatgca
     3841 ggcaaaacgg tgctgctcaa tttgtgggcg acgtggtgcg cgccctgccg cgccgagatg
     3901 ccggcgctcg atgcgctcca gaaggaaaag ggcggcgaca agttcgaggt cgtcgccgtc
     3961 aatgtcgata ccggcgacga cgccaagccg aagaagttcc tcggcgacat cggcgtcggc
     4021 aagcttggct attacagcga ccacagcatg gccgtcttca acgacgtcaa gacgcgcggc
     4081 ctggcgctcg gtcttccggt gaccatgctg gtcgatggcg aaggctgcct gatcgctcat
     4141 atgaacggtc ctgccgaatg gtcgggaggc gacgccaggc gactggtcga agcagcgatt
     4201 ggcggctaga gcggttctgg tttttaccga atcggtaaaa ccgctctatc tcttgttttg
     4261 gctgcatttc cggacacaaa accgtttcac acttttgctg gaaatgctct agaaagcagc
     4321 tctggttgct ggggaagggc gaagcggcgc atggcgtttg gctggggcat tgtcggcacg
     4381 ggcgccatag cgcgtcagtt cgcagccgat ctcacccatg cgcccggcgc gcatatcgcg
     4441 gccgtccact cgcgctcgcc tgaaaaagcc gaagcttttc gcgccgcatt caaggccgga
     4501 cgcgcctatg gcgacctcga cgccctgttg gcggacggcg cagtcgatgc cgtttatatc
     4561 gccacgccca acgcggcgca tgccgaacag gcgctgctag ccatcgccgc cggcaagccg
     4621 gtgctggtcg aaaagccgct ggcattggcc ggcgccgatg ccgggcgcat cgaggcggcc
     4681 gccttggcca aaggcacgtt cgccatggag gccatgtgga cgcgcttcct gccggcggtc
     4741 cgccgcgcca gggccaaggt cgctttgggc gaaatcggcc ccatccgcgc catccgcgcc
     4801 aatctgtcct acgttcatga cgaaacgccc ggcagccgct tctacgaacc gcaaggcggc
     4861 ggcgcgctat acgacctcgg cgtctatccg atatcgctcg ccttgcattt cctggggcat
     4921 cccgaaagcg tttccgggcg ctggtttgcg gcgcaaagcg gcgtcgacaa aagcgccgag
     4981 gtcgaacttc actacgatcg ggcggtggcc aggctatcct gcggcttcga tcgaaccggc
     5041 gagaactcgc tgctggtcga aggaacgacc gggacgctgc ggctgagggc gcccttcctc
     5101 aaggcgcagc ggctgacgca ttattcccgc gccgacagcg aaatcccgct gttggaagag
     5161 cgcgggctgt caaaaatcct cagcggctgg ctggtgccgg ggcgcaaggt ggaacagttg
     5221 cggtttcccg gcggcgggct gcaattcgag gccatcgccg tcatggacgc ggtccggcag
     5281 ggcaagacgc aatccgacct gatgccgctc gacgaaagca gggccgccct cgccatcatc
     5341 gagcaggtgc tgtcgcagcc gccctccgca gcctagaacg tgcttttcag ccgatatggc
     5401 tgcggcaggt tgcgggcatt attgagcgcc aacgccaact cggtgatgtg caacgccaga
     5461 gccccggaga attgcggctt ctgcttctcc gtgatcgcgc ttgcctgcgc ggcaatgcct
     5521 gcgcaaaaat cgatctgcga gggaaagccg ggcaatgcct tcctggccga ggtcgccggg
     5581 taagtaagcc gcctgcccac agtcggcttc cacggcagga attttccgag ccggctttcc
     5641 agccggttgg taaggcgtat tttcaggctg cggcggtcgt tttcggtttc gagatgaatc
     5701 gtggagtgtt cgtcccacag gtcgcgcacc gtaatcgatc cattgtcgcc cagaatggtc
     5761 agcgaccggt ccttcggcgc ggcaagaccc gatgtcagcc gcgccacgac accggatttg
     5821 aaggccaggc agcccaccga gaaatcgggc gccagcgcca atccttgcgt gcccggcccc
     5881 ttgtcgggaa aggccagcgc cgaaaaggcc gtgaggcttt cgaccggccc gaacagcgaa
     5941 gtgagccagg acagtgcgta accggcatgt tccagcgtgc agccgatctc gaattcatgc
     6001 gcgcccggcc atttcgcgcc ggaacgcgag cgccattcgg tccatttgtc gcgaaacacc
     6061 gcgccgtcct ccatctcggc atagacgagg cgcggcgtgc cgatgcgtcc ggcatcgagc
     6121 gcgcccgcta ccagagaatg cgcagcgctc aaggcattgg ccggcgcggt cgccagcgtc
     6181 agcccaagct cgtcggcgag ttcgaccagg atgcgcgcat cctcgaagcg catcgccagc
     6241 ggcttttcgc aatagacgtg cttgccggcc agcagcgcgc gccgggacgt ttcgaaatgg
     6301 ctttccggcg tcgtcaggtt gacgacgatg ccgacgctct cgtcggccag cagcgcctcg
     6361 acgctgtcat aggcgcggac atggtgatag tcgcaaaact gcgcaagccg cgccgcatcg
     6421 cggtcgaacg cgccggcgag ttcgagaccc gcccggttgg cgcccgagta gttgtcgcct
     6481 gagtagttgg ccaaactcgc catatagtaa tcggcgacga aacctgtgcc gagaaatgcg
     6541 attttcattg cgccacctga actgatcggc gcaaccttaa cccgaaggcg gttacatcgc
     6601 ggtaaagccc tggcctatga tgccggccgc caacgacgct ggcgctgatc cccgccctgc
     6661 gttatacgct ttcggtcttc ctgcggtgcc ctgccatgtc cttcattccc gatattgcca
     6721 cgctgctcca gttcgccatc gccactgtca tcctggcggt cacgccgggg ccggacatga
     6781 ccctgttcgt cagccgcacg ctgagccagg gccgcgcaac cggctttgcc tcgatgaccg
     6841 gcgcgctgac cggtacgctg gtccacacta cgctggtcgt cgtcggcatc tccgcgctca
     6901 tcgtcgcttc gccgatggcg tttttcgtgc tgaagatttt cggcgcgggc tatctggtgt
     6961 ttctcgcctg gcaggcgctt gccaagggtt ccgccttcac
//
LOCUS       DASYCS010000377         6176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1264416,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000377 DASYCS010000000
VERSION     DASYCS010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 6176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6176
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1264416"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..926
                     /locus_tag="VGM46_11785"
     CDS             <1..926
                     /locus_tag="VGM46_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
                     /protein_id="HEY4193317.1"
                     /translation="NYIEGLFLSSGVVRSPDETMGEMVEVARRLRLEEKFGGYIHLKT
                     IPECSAGLVEKAGLYADRLSINVELPTDEGVKRLAPEKKPETIRLSMARLRQKIEEKA
                     EPTLRTKKRERFAPGGQSTQMIIGADQTTDDGILHASARLYGSYQLRRVYYSAFSPIP
                     DSSSSLPVKKPPLMREHRLYQADWLMRFYGFSQSEILHGTADGMLDLAIDPKLAWALR
                     NRACFPVDVNRADREMLLRVPGLGAKVVKRILETRRHRRLRLEDVGRLCHSITKLRPF
                     ILAEGWSPGGLTDKAALRETIVQSCEQLTLF"
     gene            929..2401
                     /locus_tag="VGM46_11790"
     CDS             929..2401
                     /locus_tag="VGM46_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528231.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HEY4193318.1"
                     /translation="MHAQLQLTAFTVTLSGETDFSGWREAARRLVLNEIEPSDVFWQV
                     RAGEVRTREVRAALPPIPPNAVLNVPRAFVERAEAVACHRDPQRFSLLYRLLWRLRAE
                     PKLMEIASDADIRRFETMEKAVRRDIHKMRAFVRFRKIGEGDDERFVAWFEPDHFIEE
                     RNAPFFLRRFTGMRWTILTPHASIDWDGERLAAGPGAAKTDAPGEDAAEALWRTYFRN
                     IFNPARLKVKAMQKEMPKKYWRNLPEASLIPTLIAGADKAAKDMIARMPTQPAAHHAG
                     IQARHWPKDEAAPKDDGDDATSIDALRKAAKGCRRCPLWRDATQTVFGEGPDNAEVVF
                     VGEQPGDQEDIAGEPFVGPAGKVFDAILDDAGIERQKTYVTNAVKHFKFEPRGKRRIH
                     SKPNAGEVQACRWWIDHELALIKPKLVVALGATAALSLLGKAVAVTKMRGQVIERDDG
                     LRVFLTIHPSFILRIHDHDDKQAERGRFLADMKAVKKLMA"
     gene            complement(2419..3681)
                     /locus_tag="VGM46_11795"
     CDS             complement(2419..3681)
                     /locus_tag="VGM46_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEY4193319.1"
                     /translation="MNLDAKSFLTAIFNASVAAADPEKTIRGHLPARPKGRTIVIGAG
                     KGSAQMAAAFEKAWDGPIEGLVVTRYGYGAKCERIEIIEAAHPVPDKAGLEASRRLLE
                     KVENLTEDDLVVALISGGGSALLPSPAAGLTLADEIAVNEALLASGAPIAAMNTIRKH
                     LSGIKGGRLAAAAYPAKVVSLVVSDIPGDNPALVASGPTVPDAGNRQEALAAISAYGI
                     RLPDAVMEHIGSPAADAPRPDDKRFSRNEVHLIASAGVSLEAAAAEARKQGIEAVILS
                     DSIEGEAREVGSVHAAIAREVASRNRPFRKPVLILSGGETTVTLRAKGKGGRNSEFLL
                     SFAIGIAGVDGIDALAADTDGIDGSENNAGAFCDGSTVARMRAAGVDAKAMLAGNNAW
                     TAFNAAGDLFVPGPTGTNVNDLRAILIR"
     gene            complement(3846..5165)
                     /gene="guaD"
                     /locus_tag="VGM46_11800"
     CDS             complement(3846..5165)
                     /gene="guaD"
                     /locus_tag="VGM46_11800"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018429447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HEY4193320.1"
                     /translation="MPQTLLRGRTLSFVRWPENIDDAAAYAYEEDGGLLIEDGKIVAT
                     GPYAKVEKQAGEGTKKIDHRPHLILPGFIDAHAHFPQMQVIASYGAELLDWLNTYTFP
                     EETRFRNAQHGRRIARLFLDEMVRHGTTTVAAYCSVHKASAEAFFEESHARDMLNIAG
                     KVMMDRNAPEGVLDTPQSGYDDSKALISEWHGKGRQHYAITPRFAITSSPEQMEMAGA
                     LASEFPGLHVQTHLSENHAEIAFTCELYPWARDYTDVYEHYGLLGKKTLLGHSIHLSE
                     READALSDSGSVAVFCPTSNLFLGSGLFDYQRYRSRAKPLRIAAATDVGGGTNYSMLR
                     TMDEGYKVIALNGEKLNPFQSFWQITCGNAEALSLAEKVGTLDEGTDADLVVLDAGAT
                     PVMRLRMETVGTLAEELFLLQTLGDDRAVRETYVAGRAAKSDMAAGA"
     gene            complement(5168..>6176)
                     /locus_tag="VGM46_11805"
     CDS             complement(5168..>6176)
                     /locus_tag="VGM46_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HEY4193321.1"
                     /translation="APAEMPEHLTWFKWESYATLLSGLALLAIVYYAGANLFLIDPNV
                     LDVSAPVGILISLACLAFGWIVYDVLCRYLIGKADTGLMLLLYAILVFMAWGLTHLFT
                     GRAAFLHLGAITATIMAANVFMVIIPNQKIVVADLIAGRKPDAKYGKIAKQRSLHNNY
                     LTLPVLFLMLSNHYPLAFGTRFNWVIASLVFIIGVLIRHYFNTRHARKGNPTWTWLAA
                     AILFLVVIWLSTVPKILTGEQKVSQANDIYVASAHFPAVRDTVMGRCAMCHAAEPSYE
                     GIYHAPKGVMLDTDAGIAAHAEEIYLQAGRSHAMPPANVTQVTDQERALLVSWFEGAG
                     K"
BASE COUNT         1156 a         1837 c         2064 g         1119 t
ORIGIN      
        1 gcaactatat tgaggggctg ttcctgtcat cgggtgtcgt gcgctcgccc gatgaaacca
       61 tgggtgagat ggtggaggtg gcgcgcaggc tgcggctgga agaaaagttc ggcggctata
      121 ttcatctgaa gaccattccc gagtgctcgg ccgggctggt cgagaaggcc gggctttatg
      181 ccgaccggct gtcgatcaac gtcgaactgc cgaccgacga gggcgtgaag cggctggcgc
      241 cggaaaagaa gccggaaacg atccggcttt cgatggccag gctgcgccag aagatcgagg
      301 aaaaggccga accgacgctg cgcaccaaaa agcgcgagcg tttcgcgccg ggcggccagt
      361 cgacgcagat gatcatcggg gcggaccaga ccacggatga cggcatcctg catgccagcg
      421 cgcggcttta cggcagctat cagttgcggc gggtctatta ttcggcgttc agccccattc
      481 ctgattcttc gtcttcgctg ccggtcaaaa agccgccgct tatgcgtgag catcggcttt
      541 accaggccga ttggctgatg cggttctatg gattttcgca aagcgagatc ctgcatggaa
      601 ctgccgacgg catgctcgac ctggccatcg atccgaagct cgcctgggcg ctgaggaacc
      661 gcgcctgctt tcccgtcgac gtaaaccgcg ccgaccgcga gatgctgcta cgcgtgccgg
      721 gcttgggcgc aaaggtcgtc aagcgcatcc ttgagacgcg gcggcaccgg cggctgcggc
      781 tggaggatgt cgggcggctt tgccattcca tcaccaagct gcggcccttc atccttgccg
      841 aaggctggtc gccgggtggc ctgaccgaca aggcggcgct gcgcgaaacc atcgtgcaat
      901 cctgcgaaca actgacgctg ttttgaccat gcacgcgcag cttcagctaa ccgcctttac
      961 cgttacgctg tcgggcgaaa cggatttttc cggctggcgc gaggcagccc ggcggctggt
     1021 gctcaacgaa atcgagccga gcgatgtatt ctggcaagta cgggctggtg aggtaaggac
     1081 tcgtgaggta agggccgctc tgccgccgat cccgccaaat gccgtgctca atgtgccgcg
     1141 cgctttcgtc gagcgcgccg aagcggtggc ctgtcatcgc gacccgcagc ggttttcctt
     1201 gctctaccgg ttgttgtggc ggttgagggc cgaaccgaag ctgatggaaa tcgcttccga
     1261 tgcggacatt cgccgcttcg agacgatgga aaaggcggtg cgccgcgaca ttcacaagat
     1321 gcgcgccttc gtgcgttttc gaaagatcgg cgaaggcgat gacgagcgct tcgtcgcctg
     1381 gttcgaaccg gatcatttca tcgaggagcg caacgcgccg tttttcttgc ggcgtttcac
     1441 cggcatgcgc tggacgatcc tgacgccaca cgcctcgatc gactgggatg gcgaaaggct
     1501 ggccgcagga ccgggcgcgg caaagaccga cgcgcccggc gaagacgcgg ccgaggcgct
     1561 gtggcggacc tatttccgca acatcttcaa tccggcgcgt ctgaaggtga aggccatgca
     1621 aaaggaaatg ccgaagaaat attggcggaa ccttcccgag gcgtcgctca ttcccacctt
     1681 gattgcagga gcggacaagg ccgcgaagga catgatcgcc agaatgccga cgcaacccgc
     1741 cgcccatcat gccggaatac aggcaaggca ttggccgaag gacgaggccg caccgaaaga
     1801 tgacggcgac gacgcaacat ccatcgatgc gttgcgcaaa gccgccaaag gctgccgccg
     1861 ctgcccgtta tggcgcgatg cgacgcagac cgtgttcggt gaggggccgg acaatgccga
     1921 ggtggtgttt gtcggcgagc agccgggcga ccaggaagat attgccggcg agccgtttgt
     1981 cgggccggcg ggaaaggtgt tcgacgcgat cctcgacgat gccggcatcg agcggcagaa
     2041 aacctacgtc accaatgcgg tgaagcattt caagttcgag ccgcgcggca agcgccgcat
     2101 ccattccaaa ccgaatgccg gcgaggtgca ggcctgccgc tggtggatcg accacgaact
     2161 ggcgctgatc aagccgaagc tggtcgtcgc gctgggagcg acagcagcgc tttcgctgct
     2221 gggaaaggcg gtcgcggtga cgaagatgcg cggccaggtg atcgagcgcg acgacggttt
     2281 gcgcgtcttc ctgaccatcc acccatcttt catcctgcgc atccacgacc atgacgacaa
     2341 gcaagccgag cgcgggcgtt tcctggccga tatgaaagcg gtaaagaagc tgatggcctg
     2401 agttaatggc ctgagcggct agcggatcag gatcgcccgc aggtcgttga cattagtgcc
     2461 tgtcggtccc ggcacgaaca ggtcgccggc ggcattgaac gccgtccagg cattgttgcc
     2521 ggccagcatc gccttggcgt cgacaccggc ggcgcgcatg cgcgccaccg tcgagccatc
     2581 gcaaaacgcg ccggcattgt tctccgaacc atcaatgccg tcggtatcgg cggcaagcgc
     2641 gtcgatgccg tcgaccccgg caatgccgat ggcgaaggaa agcaggaatt cggaattgcg
     2701 gccgcccttg cccttggcgc gcaaggtcac cgtggtttcg ccgcccgaca ggatcagcac
     2761 cggctttctg aaaggccggt tcctcgaagc cacttcgcgc gcaatcgcgg catggacgga
     2821 gccgacctcg cgtgcctcgc cttcgatgga atccgacagg ataaccgcct cgatgccctg
     2881 ctttcgggct tcggcggcgg cggcttccag cgacactccg gcggaggcaa tcaggtgaac
     2941 ctcgttgcgc gaaaagcgtt tgtcatcggg gcgtggcgca tcggcggcag gcgagcctat
     3001 atgttccatg acggcgtcgg gcagcctgat gccgtaggcc gagatggcgg ccagcgcttc
     3061 ctgccggttg ccggcgtcgg gcactgtcgg gccggaggcg acaagcgccg gattgtcgcc
     3121 cggaatatcg gagacgacca gcgacaccac cttggccggg taggcggctg cggccagccg
     3181 gccgcccttg atgccggaga gatgcttgcg gatggtgttc atggcggcaa tcggcgcgcc
     3241 ggaggcgagc agcgcctcgt tgacggcgat ctcatcggcc agcgtcaggc cggcggccgg
     3301 cgacggcaac agggcggaac caccaccgga aatcagcgcg acgacgagat cgtcttcggt
     3361 gaggttttcc accttttcaa gcagccggcg cgaggcttcg aggccggcct tgtcgggaac
     3421 ggggtgcgcc gcctcgataa tctcgatgcg ctcgcatttg gcgccgtagc cgtagcgggt
     3481 gacgaccagt ccctcgatcg gcccgtccca tgccttttcg aaggcggcgg ccatctgcgc
     3541 cgatcccttg cccgcgccga tgacgatcgt gcggcccttc ggcctggccg gcaaatggcc
     3601 gcgaatggtc ttttccggat cggcggcggc gacagacgcg ttgaagatgg cggtcaggaa
     3661 ggatttggca tcgaggttca tgtggtttcc agcgacattg tccgtgcttg ggaatccggc
     3721 gcattcagcg cagcgacgat cggagggacc gacggcgacg cccaaattcc ctccctgtgc
     3781 gccgccgctc gctttgtgac gacaaggttc cgcgcggtca agccgtgacc ttcagctgaa
     3841 ggcgcttatg cgccagcagc catgtcgctt ttagcggccc tgcccgcgac ataggtttcg
     3901 cgcaccgcgc ggtcgtcgcc cagcgtttgc agcaggaaca gttcctcggc cagggtgcct
     3961 accgtttcca tgcgaaggcg catgactggc gttgcgcccg catccaacac gacgagatcg
     4021 gcgtcggtgc cttcatccag cgtgccgacc ttctcggcca atgacaacgc ctcggcgttg
     4081 ccgcacgtga tctgccagaa ggactggaac gggttcagct tctcgccatt caacgcgatg
     4141 accttgtagc cctcgtccat ggtgcgcaac atggaatagt tggtgccgcc gccgacatcg
     4201 gtggcggcgg cgatgcgaag cggtttggcg cggctgcggt agcgctggta atcgaacagg
     4261 ccggaaccga ggaacaggtt ggaggttggg cagaaaaccg cgacggagcc gctgtcggac
     4321 aaggcgtcgg cctcgcgttc cgacagatgg atcgagtgcc cgagcagggt ctttttgccg
     4381 agcaagccgt aatgttcgta aacgtcggta tagtcgcgcg cccatggata aagttcgcag
     4441 gtgaaggcga tttcggcgtg attttccgac agatgcgtct ggacatgcag gccgggaaac
     4501 tcgctggcca gcgcgcccgc catttccatc tgttcgggcg acgaggtgat ggcgaagcgt
     4561 ggcgtgatcg cataatgctg gcggcccttg ccatgccatt cggagatcag cgctttactg
     4621 tcgtcatagc cggattgcgg cgtgtccagt acgccctccg gcgcgttgcg gtccatcatc
     4681 accttgccgg cgatgttgag catgtcgcgg gcgtggcttt cctcgaaaaa cgcctccgcc
     4741 gaagccttgt gcacagaaca gtaggcggcg accgtggtgg tgccgtgccg caccatctcg
     4801 tccaggaaca ggcgcgcgat gcggcggccg tgctgggcgt tccggaacct ggtttcctcc
     4861 gggaaggtat aagtgttcag ccagtcgagc aattcggcgc catagctggc gatgacctgc
     4921 atctgcggaa agtgggcatg cgcatcgata aaaccgggca aaatgagatg cgggcggtga
     4981 tcgatctttt tcgtcccctc gcccgcttgc ttctcgacct tggcgtaagg gccggtagcg
     5041 acgatcttgc cgtcttcgat gagcaggccg ccatcttcct cgtaagcata ggcagcggcg
     5101 tcatcgatgt tttccggcca gcgaacaaag gaaagggtgc ggccgcgcaa aagggtctgt
     5161 ggcatgctta ttttccagca ccttcgaacc agcttaccag cagcgcgcgc tcctgatcgg
     5221 tgacttgcgt gacgttggcg ggcggcatgg cgtggctgcg gccagcctga agataaatct
     5281 cctcggcatg tgcggcgatg ccggcatcgg tgtccagcat cacgcctttc ggggcatggt
     5341 agatgccctc ataggacggc tcggcggcgt ggcacatggc gcagcgcccc atcaccgtat
     5401 cgcgcaccgc cgggaaatgt gcggaggcga catagatgtc gttggcctgc gagaccttct
     5461 gttcgccggt caggattttc gggaccgtcg acagccagat gacgacgagg aacaggatgg
     5521 cggcggccag ccacgtccag gtcgggttgc ccttgcgggc atgccgcgtg ttgaaataat
     5581 ggcggatcag cacgccgatg atgaagacca gcgaggcgat gacccagttg aaccgcgtgc
     5641 cgaacgccag cggatagtgg ttcgacagca tcaggaacaa aacgggcagc gtgaggtagt
     5701 tgttgtgcag cgagcgctgc ttggcgatct tgccatattt cgcatcgggc tttctgcccg
     5761 cgatcaggtc ggcgacgacg atcttctggt tggggatgat gaccatgaag acgttggcgg
     5821 ccatgatggt ggcggtgatg gcgccgagat gcaggaaggc ggcgcgcccg gtgaacaggt
     5881 gcgtcagccc ccaggccatg aacacgagaa tggcgtagag caaaagcatc aagccggtat
     5941 cggccttgcc gatcagatac cggcacagca cgtcatagac gatccagccg aaggccaggc
     6001 aggcaagcga aatgaggatg ccgaccgggg ccgagacgtc gagaacgttg ggatcgatga
     6061 ggaagaggtt cgcgccggcg taatagacga tggcgagcag ggccaggccc gacagaagcg
     6121 tggcgtagct ctcccatttg aaccatgtca ggtgttcggg catttcggcg ggcgcc
//
LOCUS       DASYCS010000378         9397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4312194,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000378 DASYCS010000000
VERSION     DASYCS010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 9397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9397
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4312194"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..132
                     /locus_tag="VGM46_11810"
     CDS             <1..132
                     /locus_tag="VGM46_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP:cob(I)alamin adenosyltransferase"
                     /protein_id="HEY4193322.1"
                     /translation="AAEHVNPEGIKYINRVSDLLFVAARAVNDNGNGDVLWVPGENR"
     gene            162..887
                     /locus_tag="VGM46_11815"
     CDS             162..887
                     /locus_tag="VGM46_11815"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008598098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEY4193323.1"
                     /translation="MFIPLYDTNHLRTIRLQYVTFVLIGINVAVYLATTIGGENFNNA
                     AVLGLSFIPSVVHHKAELAPQFVIIPENLSYLTYAFLHADIFHLGGNMLFLWVFGDNV
                     EDALGHFRYLLFYVACAIGGAAFQGLIAPDSQTPLIGASGAIAGVVVAYLILYPRVKI
                     WILAFSRIPLHIPAYIPLALWILVQFVMFGLGGDEQISWACHIGGILSGTALVLLLRR
                     RNVPLTDEVIVTPEPVEATDIDG"
     gene            1049..1798
                     /locus_tag="VGM46_11820"
     CDS             1049..1798
                     /locus_tag="VGM46_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEY4193324.1"
                     /translation="MKVLVPVKRVVDANVKVRVKADGSAVELANVKMAMNPFDEIAVE
                     EAIRLKEAGKVEEIIVVSIGPQQAQETLRTALAMGADRAILVKTDEQTEPLGVAKVLK
                     GVVEAEQPGLVILGKQAIDDDSNQTGQMLAALLGWAQGTFASKIELAGDKAKITREVD
                     GGLQVVELKMPAIVTADLRLNQPRYASLPNIMKAKKKPLDEKTPADFGADVKPRLKVL
                     KTEEPSGRKAGVKVKSVAELVDKLRNEAGVL"
     gene            1823..2752
                     /locus_tag="VGM46_11825"
     CDS             1823..2752
                     /locus_tag="VGM46_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002965036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEY4193325.1"
                     /translation="MAILLIAEHDNATLSDQTAKALSAALQIGPDVHVLVAGKGAKGA
                     ADAAAKLKGVGKVLLAEADELAQQLAEPLAALVVGIAGGYDAIVAAATSTGKNVSPRI
                     AALLDVAQISEIIEIVSPDTFKRPIYAGNAIQTVQSGEAKKVITVRTAGFQAAGEGGS
                     ASVETISAAGNPGLSTFVENRLSESDRPELTSARIIISGGRALGSSEKFQEVILPVAD
                     KLGAAVGASRAAVDAGYAPNDWQVGQTGKVVAPDLYIACGISGAIQHLAGMKDSKVIV
                     AINKDEEAPIFQVADYGLVADLFIALPELEKAL"
     gene            2914..3792
                     /locus_tag="VGM46_11830"
     CDS             2914..3792
                     /locus_tag="VGM46_11830"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HEY4193326.1"
                     /translation="MSKIETIGIIGSGQMGSGIAHVSALSGYNVLIYDISVDRIEKGI
                     ATINGNMARQVGAGKLDEQERKQAMARISTAASMADLAAADLVMEAATEDETVKRKIY
                     AQLCPQLNPEAILATNTSSISITRLAAQTDRPERFIGIHFMNPVPLMKLVELVRGIAT
                     EDQTFEAAKAYVSRLDKAITVSEDFPAFIVNRILLPMINEAIYTLYEGVGSVEAIDTA
                     MRLGANHPMGPLQLADFIGLDTCLSIMQVLHDGLSDSKYRPCPLLVKYVEAGWLGRKT
                     GRGFYDYRGEHPVPTR"
     gene            complement(3818..4114)
                     /locus_tag="VGM46_11835"
     CDS             complement(3818..4114)
                     /locus_tag="VGM46_11835"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY4193327.1"
                     /translation="MDMQQDIRREEQNGRGRYVLAMSDGQEAYLRFQRSSPDNIVIDY
                     SFVPPADRGRGVAAMLILRAVQDAREAQTTITPTCGYVVAEFRRHAEWTDVLKR"
     gene            complement(4201..4500)
                     /locus_tag="VGM46_11840"
     CDS             complement(4201..4500)
                     /locus_tag="VGM46_11840"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY4193328.1"
                     /translation="MTDQLPEIEFEDNGSKGRYILRGPNGEEAETTFTKIGDTKIIID
                     HTDVPDSFRGQGAGLRLVTRVVEDARAAGKKIIPLCPFAAAQFQRHREWADVLTR"
     gene            complement(4497..5126)
                     /locus_tag="VGM46_11845"
     CDS             complement(4497..5126)
                     /locus_tag="VGM46_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018429823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY4193329.1"
                     /translation="MDANAYIHKALPGVPGGPLLFVFHGTGADENQLPGLGRELMPDA
                     SVVSPRGDVSEHGAARFFRRTGEGVYDMADLARATAKMAGFIHAHVEARQPSSVVGLG
                     YSNGANILASVLFAEPSLFGAAVLMHPLIPFTPDIKGDLAGKRILLTAGRHDPICPPD
                     LTERLERNLRCAQADIKVEWHEGGHEVRPSEIEAARLFFGRRQDERQAR"
     gene            complement(5136..6068)
                     /locus_tag="VGM46_11850"
     CDS             complement(5136..6068)
                     /locus_tag="VGM46_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY4193330.1"
                     /translation="MLDQIKGLHHVTSMARDARGNNDFFTHKLGLRRVKQTVNFDAPD
                     VYHLYYADELGTPGSVMTYFPFPNIGKGRPGAGEVGTSVFSVPEGTLAYWEKRFADQG
                     VGGLSRQETFGEKRLAFHGPDGDGFALVEDKADRRAPWAKGGVPADEAIRGFHSVSLR
                     LRDAGATEELLKFMGYEEVDRSGDTRRLAIAGGNGADIVDIETLPGAPAAGLGAGSVH
                     HVAFAVENRAKQLEVRKALADTGYQVTPMIDRDYFWSIYFRTPGGVLFEIATNEPGFD
                     RDEDTAHLGEALKLPKQHEHLRTYLEQHLQPLEG"
     gene            complement(6277..7638)
                     /locus_tag="VGM46_11855"
     CDS             complement(6277..7638)
                     /locus_tag="VGM46_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEY4193331.1"
                     /translation="MIRTLMATTALATLLVSGAYAQDATAPAETTAPAVQQSAPAAIV
                     TPADGTIATNIIGESVYNGTADDAESIGKVSDIVLDESGKVKSLVIGVGGFLGLGEKN
                     VAYDFTKLQWAEKNGDRWLVAETSKDALKAQPAFDSKPYEPASNAAASDTTMAPADTT
                     TSSDATPAPSSDTAQTAPAATDAQPVVKADGNLASLIMGENVYNGTSDDAQNIGDVND
                     IVFTKDGQAESLVIGVGGFLGVGQKSVAFDYDKAKWASKADDRWLVVEGTKEELQALP
                     AFDRKAFDPAPAPAAAANDVAAPAPMATAPADTATTAPADTATTPAPSTDTAAGTDTT
                     ETAAIDKSTLTELPIGETTSQNLVGATVYGADDAKIGEIGDIALSADQKVDAVIVDVG
                     GFLGVGEKQVAVGMDNLKFMADKDGNKYLYTSFTKDQLEKQAAYDKGTYGEKRDDQRL
                     MMQ"
     gene            7891..8808
                     /locus_tag="VGM46_11860"
     CDS             7891..8808
                     /locus_tag="VGM46_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEY4193332.1"
                     /translation="MKFAAVLNRSGGTLRTTDIPAFRDRMRETLEAAGHCVVVEVVDG
                     EDVSSALDEAVSSRGVDVILAGGGDGTISAAAARLMDKKKALAVLPAGTMNLFARGLG
                     IPQGLDAAVKAFATGEVVSFDMASANGQPFVHQFSIGMHAKMVQLREKMDFGSRLGKL
                     RASARAAWSTINNPPAMDVSLTVGEAEIVARTSGIGITNNLFGERHLPYADNPAGGVL
                     GIYVTMTRQRGELVHYFFNVARGKWRDNQHVEIHQADKAVLKVGSPGKRLRAVMDGEL
                     VRLEPETVFEIHPKALRVLVPSVEQEDKA"
     gene            complement(8857..9048)
                     /locus_tag="VGM46_11865"
     CDS             complement(8857..9048)
                     /locus_tag="VGM46_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193333.1"
                     /translation="MTKIIPEQKARQAGWGKRVLLILICALVLIVIAWAAAEFYGETI
                     DNSTPAADTTTSQGQMPKG"
     gene            complement(9188..>9397)
                     /locus_tag="VGM46_11870"
     CDS             complement(9188..>9397)
                     /locus_tag="VGM46_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193334.1"
                     /translation="RETRRRKADMTAIGVTTALAVLPALLRGKGGVGALIAPAVAAAA
                     YAIYRENTKPQDGSDKDGPDKAGRQ"
BASE COUNT         1713 a         2887 c         2957 g         1840 t
ORIGIN      
        1 gccgccgagc acgtcaatcc cgaaggcatc aaatacatca accgggtgtc cgacctgctg
       61 ttcgtggccg cgcgcgccgt caatgacaac ggaaatggcg atgtgctatg ggtgcccggt
      121 gagaaccgct gacatttccg ccggcgagcg gggagccttc gatgtttatc ccgctctatg
      181 acaccaacca cctgcgcacc atccggctgc aatatgtcac gttcgtgctg atcggcatca
      241 atgtcgccgt ctatcttgcg acgacaatcg gcggcgagaa tttcaacaat gccgccgtgc
      301 tcggcctgag cttcattccc tcggtggttc accacaaggc ggaacttgcg ccgcaattcg
      361 tcatcattcc cgaaaatctg agctacctga cctacgcttt cctgcatgcc gacattttcc
      421 atctcggcgg taacatgctg tttctgtggg tgttcggcga caatgttgaa gatgcgcttg
      481 gccattttcg ctacctgctg ttctacgtgg cctgcgccat cggcggcgcc gccttccagg
      541 gcctgatcgc gcccgattcg cagacgccgc tgatcggcgc ctcgggggcg atcgccggcg
      601 tggtggtggc ctatctcatt ctttatccgc gcgtgaagat atggatactc gccttcagcc
      661 gcattccctt gcacattcca gcctatattc cgctggcgct gtggatattg gtgcagttcg
      721 ttatgttcgg gcttggcggc gacgagcaga tttcctgggc ctgccacatt ggcgggatac
      781 tgtccggaac ggcgctggtg cttttgctgc gacgccgcaa cgtgccgttg acggatgagg
      841 tcatcgtcac gccggaacct gtcgaggcga cggatattga cggatagaca tgccgcaatt
      901 acggtccgcg cgcttgtgga agcccctccg ccaccactga gacggctgaa caacggtatt
      961 tccatgaaaa atgtcggctt tcgacaactt cgaagaagcg cgcgctgcta cggctcgctc
     1021 caaaccattc tacagaggtg acaagcagat gaaagttctg gtgcccgtga agcgggttgt
     1081 cgatgcgaat gtaaaggtcc gggtcaaggc tgacggatcg gcggtcgaac tggccaatgt
     1141 gaagatggcc atgaacccgt tcgacgaaat cgcggtcgaa gaggccattc ggctgaagga
     1201 agccggcaag gtcgaggaga tcatcgtcgt ttctatcggg ccgcagcagg cgcaggaaac
     1261 gctgcgcacg gcgctcgcca tgggcgcgga ccgtgccatc ctggtcaaga ccgacgaaca
     1321 gaccgaaccg cttggcgtcg ccaaggtgct gaagggcgtg gtcgaggccg aacagccggg
     1381 cctggtcatt ctcggcaagc aggccatcga cgacgattcc aaccagaccg gccagatgct
     1441 ggccgccctg ctcggctggg cgcagggcac ctttgcctcc aagatcgagc ttgccggcga
     1501 caaggcgaag ataacgcgcg aagtcgatgg cggcttgcag gtggtggagc tgaagatgcc
     1561 ggccatcgtc accgccgacc ttcgcctcaa ccagccgcgc tacgcttcgc tgcccaacat
     1621 catgaaggcc aagaagaagc cgctggacga aaagaccccg gccgatttcg gagccgacgt
     1681 taagccgcgc ctcaaggtgc tgaagaccga agagccgagc ggccgcaagg cgggcgtcaa
     1741 ggtcaagagc gtcgccgaac tggtcgacaa gctgcgcaac gaagccggcg tgctgtgatc
     1801 ggcggaagat aggagacaaa aaatggctat tcttctgatt gcagaacacg acaacgcgac
     1861 gctttccgac cagaccgcaa aggcgctttc ggcggccttg cagatcggcc cggacgtgca
     1921 tgtgctggtt gccggcaagg gcgcaaaggg cgccgccgat gccgctgcca agttgaaggg
     1981 cgtgggcaag gtgctgctgg ccgaggccga cgagcttgcc cagcagctgg ccgaaccgct
     2041 ggcggccctg gtcgtcggca tcgccggcgg ctacgacgcc atcgttgccg ccgccacctc
     2101 gaccggcaag aacgtttctc cgcgcattgc agccttgctg gatgtcgcgc aaatttcgga
     2161 aatcatcgaa atcgtttcgc ccgatacctt caagcggccg atctatgccg gcaacgccat
     2221 ccagaccgtg cagtcgggtg aggccaagaa ggtcatcacc gtgcgcaccg ccggcttcca
     2281 ggcggcgggc gaaggcggtt cggcttcggt cgaaaccata tcggcggccg gcaatcccgg
     2341 cctgtcgacc ttcgtcgaga accgcctgtc ggaaagcgac cgtccggaac tgacttccgc
     2401 caggatcatc atttccggtg gccgcgcgct cggctcttcg gaaaaattcc aggaagtcat
     2461 cctgcctgtc gccgacaagc tgggcgccgc cgtcggcgcc tcgcgcgccg ccgtcgatgc
     2521 cggctacgct cccaacgact ggcaggtcgg ccagaccggc aaggtggtgg ccccggacct
     2581 ttacattgcc tgcggcattt ccggcgccat ccagcacctt gccggcatga aggattcgaa
     2641 ggtcatcgtc gccatcaaca aggacgagga agcgccgatc ttccaggttg ccgactatgg
     2701 cctggtcgcc gacctgttca tcgccctgcc ggaacttgaa aaggcgcttt gacgctttcg
     2761 gcccgtccgt attacattga tccgcgaaag gcggggaggc agacgccttc ccgccttttt
     2821 tgggtctgtc gaagacccgg ttgcgctgca caagaaatag gctagtttgc actgcacaaa
     2881 atgtgcctgg gctgcgtttg acgggatcaa gtgatgagta agatcgagac aatcggcatt
     2941 atcggatctg gccagatggg cagcggcatt gcccatgtat cggcgttgtc cggctataat
     3001 gtgctgatct acgacatttc ggtcgaccgc atcgaaaaag gcatcgccac catcaatggc
     3061 aacatggcgc gccaggtcgg cgccggcaag ctggacgagc aggagcgcaa gcaggcgatg
     3121 gcccgcatct cgactgccgc ctcgatggcc gatctcgccg ccgccgatct cgtcatggag
     3181 gccgccaccg aggacgaaac ggtcaagcgc aagatttacg cgcagctttg tccgcagctc
     3241 aatcccgagg cgatcctcgc caccaacaca tcgtcgattt ccattacccg gcttgccgcg
     3301 cagaccgacc ggcccgagcg cttcattggc atacatttca tgaacccggt tccgctgatg
     3361 aaactggtcg agctggtgcg cggcatcgcc accgaagacc agaccttcga ggccgcgaaa
     3421 gcctatgtga gccgtctcga caaggcgatc acggtgtcgg aggattttcc cgccttcatc
     3481 gtcaaccgca tcctgttgcc gatgataaac gaggcgatct acacgcttta cgagggcgtc
     3541 ggctccgtcg aggccatcga tacggcgatg aggctgggcg ccaaccatcc gatggggccg
     3601 ctgcaactgg ccgatttcat cggtctcgat acctgcctgt cgatcatgca ggtgctgcat
     3661 gacggtctgt cggactccaa ataccgtccg tgtccgctgc tggtgaaata tgtcgaggcg
     3721 ggctggcttg gccgcaagac gggccgcggc ttctacgact atcgcggcga gcaccccgtt
     3781 ccgacgcgct aagggggcat tttcgggccg taactgccta acgcttcagc acgtccgtcc
     3841 attccgcatg gcggcggaac tcggccacga catagccgca ggtcggggtg atggtggttt
     3901 gcgcttcgcg cgcatcctgc acggcgcgca ggatcagcat cgcggcaacg ccgcggcccc
     3961 ggtcggccgg cggcacgaaa ctatagtcga tgacgatatt gtccgggctt gaccgctgga
     4021 accgcagata tgcttcctgc ccatcggaca tggcaagcac atagcggcca cggccatttt
     4081 gttcttcccg gcggatgtct tgctgcatgt ccattggatt gcctgtcacg atgccgtttt
     4141 ttttgggccg ttttctggaa atgtccgagc gcctgcaagg ttctcgcgcc gaatggcggg
     4201 ctagcgcgtc agcacgtcgg cccattcccg gtggcgctgg aattgggcgg ctgcaaacgg
     4261 gcaaagcggg atgatcttct tgccggcggc gcgcgcatcc tcgaccacgc gggtaaccag
     4321 gcgcagcccc gcgccttggc cgcgaaagga gtccggcaca tcggtgtggt cgatgatgat
     4381 cttggtgtcg cctatcttgg tgaaggtcgt ctccgcttcc tcgccattgg ggccgcgcag
     4441 aatatagcgg cctttcgagc cattatcctc gaattcaatc tcgggcagtt gatccgtcat
     4501 cgcgcttgcc tttcatcctg ccggcggccg aaaaacagcc ttgccgcctc gatctcgctt
     4561 ggccgcacct cgtggccgcc ttcgtgccat tcaactttga tatcggcctg cgcgcaacgc
     4621 aaattgcgct caagccgttc cgtcaggtcc ggcgggcaga tcggatcgtg gcggcctgcg
     4681 gtcagcagaa tccgcttgcc ggccaagtcg ccttttatgt cgggcgtaaa cggtatcagc
     4741 ggatgcatca acacggccgc gccgaacagt gaaggctcgg caaacaatac cgaggccagg
     4801 atattggcgc catttgaata gccaaggccg actaccgacg atggctgccg cgcctcgaca
     4861 tgggcgtgga tgaaaccggc catcttcgcc gtcgcgcggg caagatcggc catgtcatag
     4921 acgccctcgc cggtgcggcg gaaaaagcgc gccgcgccat gttccgaaac atcgccgcgc
     4981 ggcgaaacca cgctggcatc gggcatcagc tcccggccaa ggcccggcaa ctggttctcg
     5041 tccgcgccgg tgccatggaa aacgaacagc agcggaccgc cgggcacacc gggcagcgcc
     5101 ttgtggatat aggcattggc gtccatcgct tcgcctcacc cttccagcgg ctgtaggtgc
     5161 tgttcgagat aggtccgaag atgctcgtgc tgcttgggca gtttcagcgc ctcaccgaga
     5221 tgggcggtgt cctcgtcgcg atcgaagccc ggctcgttgg tggcgatctc gaacagcacg
     5281 ccgcccggcg tgcggaaata gatcgaccag aaatagtcgc gatcgatcat cggcgtgacc
     5341 tgatagccgg tgtcggccag cgccttgcgg acctccaact gcttggccct gttttccacg
     5401 gcgaaggcga cgtggtgaac ggagccagcg ccaagcccgg cagcgggggc gccgggcagc
     5461 gtctcgatat cgacgatgtc ggcgccgttg ccgcctgcga ttgccagccg ccgggtgtcg
     5521 ccggagcgat ccacttcctc atagcccatg aatttcagca gttcctccgt cgcgccggca
     5581 tctcgcaaac gcagcgagac cgagtgaaag ccgcgaatag cctcgtcggc gggaacgccg
     5641 cccttcgccc agggcgcacg gcggtcggcc ttatcctcga caagcgcgaa accatcgccg
     5701 tcggggccgt ggaacgccag ccgcttttcc ccgaacgtct cctggcggga aagaccgccg
     5761 acgccttgat cggcaaagcg cttctcccaa taggcgagcg tgccttccgg caccgagaaa
     5821 acgctggttc caacctctcc cgcgccggga cggcccttgc cgatattcgg aaacgggaaa
     5881 taggtcatca ccgaaccggg cgtgccaagc tcgtcggcat agtagagatg atagacgtcc
     5941 ggtgcgtcga aattgaccgt ctgcttcacc cggcgcaggc caagcttgtg cgtgaaaaaa
     6001 tcattgttgc cccgcgcgtc cctcgccatc gaggtgacgt gatgcaaacc cttgatctgg
     6061 tcgagcatga tgctggtcct cctgtgctgc ggcacccgcc gccgtccagc ccaatatgag
     6121 catagtgcga ccagtggcaa aggcggcaaa cacagcaaga actgtgctga aaaggtgaac
     6181 aatgaggatt gcatcgttca ctcggcctgc aaaaaagtag gcccgcgcca tggcggcgcg
     6241 ggcctttttt ctctcgtcag gatgtgggcg aagcgcttac tgcatcatca ggcgctgatc
     6301 gtcgcgcttc tcgccatagg tgcccttgtc ataggcggcc tgcttttcca actggtcctt
     6361 ggtgaaggac gtgtagagat atttgttgcc atccttgtca gccatgaatt tgagattatc
     6421 catgccgacg gcgacttgct tttcaccgac gccgaggaac ccgccgacat ccacgattac
     6481 ggcatcgacc ttctggtcag ccgaaagggc gatatcgcca atctcgccta tcttggcgtc
     6541 gtcagcgccg taaacggtgg cgccgaccag gttctggctg gtggtctcgc caatgggtag
     6601 ctcggtcaat gtcgacttgt cgatcgccgc cgtttccgtg gtgtcggtgc cggcggccgt
     6661 atcggtcgaa ggcgcgggcg tggtcgccgt atcagccgga gcggttgtcg ccgtatcggc
     6721 tggcgccgtc gccatggggg caggggcggc tacatcgttt gccgcagcag cgggagccgg
     6781 agcggggtcg aaggcctttc ggtcgaacgc cggcagcgcc tgcaattctt ccttggtgcc
     6841 ttccacgacc agccagcggt catccgcctt gctggcccat ttcgccttgt cgtagtcgaa
     6901 ggccacgctt ttctggccga cgcccagaaa gccgccgacg ccgataacca gcgactcggc
     6961 ctgaccgtcc ttggtaaaga cgatatcgtt gacgtcgccg atattctggg cgtcgtcgct
     7021 ggtcccgtta tagacgttct cgcccatgat caggctggca aggttgccgt cggccttgac
     7081 gacgggctgg gcgtcggtcg ccgccggtgc ggtctgcgcg gtgtcggagg acggcgcagg
     7141 cgtcgcatcg ctcgacgtgg tggtgtcggc cggggccatg gtcgtgtcgc tggcggcggc
     7201 attgctggcc ggctcatagg gcttggaatc aaaagccggc tgcgccttca gcgcatcctt
     7261 gctggtttcg gcgaccagcc agcggtcgcc gttcttttcg gcccattgca gcttcgtgaa
     7321 atcataggcg acgttcttct cgccgaggcc gaggaagccg ccgaccccga tgaccagcga
     7381 ttttaccttg ccgctctcat cgagcacgat atcgctgacc ttgccgatgc tttcggcatc
     7441 gtcagcggtt ccgttataga cggattcgcc gatgatattg gtggcgatgg tgccatcggc
     7501 gggcgtcacg atcgcggcgg gagccgattg ctgaaccgcc ggagccgtgg tttcggccgg
     7561 cgcggtggcg tcttgagcat aggcgcccga cacaagcagc gtggccagtg ccgtcgtagc
     7621 cataagtgtg cggatcatga tagtgtctcc ttgtgcgtga aagcgtgcag gccgcgtccg
     7681 gcggaaccgt cgctgggaat atcgggcgcg gcattctaca ggctcacaac gccgtgatcg
     7741 cctatctgtt ccgaatttgt cgccgttgga tcgcgccgat tgtctgagaa cgccaggccc
     7801 tcgaatggac cgggcaactt tccggcggat ccgccgttac cttacgaacc ggccatccgg
     7861 catgggtatt tgtcgcaata tggggtgcat atgaaatttg ctgcggtgct gaaccggagc
     7921 gggggaaccc tgcgaaccac ggatattccc gctttccgcg accggatgcg cgaaacgctg
     7981 gaagcagccg gacattgcgt cgtcgtggaa gtcgtcgacg gcgaggacgt atcctccgcg
     8041 ctggacgaag cggtgtcgtc gcggggcgtc gatgtcattc ttgccggcgg cggcgacggc
     8101 accatctcgg cggcggcggc gcggttgatg gacaagaaga aggcgctggc ggtgcttccc
     8161 gccggaacca tgaacctgtt tgcgcgcggg cttggcattc cgcagggcct ggacgcggcg
     8221 gtaaaggctt ttgcgaccgg cgaagtcgtt tcgttcgata tggcgagcgc caacggccag
     8281 ccattcgtcc atcagttttc gatcggcatg catgccaaga tggtgcagtt gcgcgaaaag
     8341 atggatttcg gctcgcggct cggcaagctg cgcgcctccg cgcgcgctgc gtggtccacc
     8401 atcaacaacc cgccggccat ggatgtcagc ctgacggtgg gggaagcgga aatcgtcgcg
     8461 cgcaccagcg gtataggcat caccaataac ctgtttggcg agcgccatct gccctacgcc
     8521 gacaatccgg cgggcggcgt tctgggaatt tacgtgacga tgacccgcca gcgcggcgag
     8581 cttgtgcatt atttcttcaa cgtggcacgt ggaaaatggc gcgacaacca gcatgtggaa
     8641 atccaccagg ccgacaaagc ggtcctcaag gtaggttcgc cgggcaaaag gctgcgcgct
     8701 gtgatggacg gcgaacttgt caggctggaa ccggagacag tctttgaaat ccaccccaag
     8761 gcactaaggg tgctggtgcc atcggtcgag caagaggaca aggcttaggc aaaaaggccg
     8821 gacctaggga aagagggggc gtaggctcgg tccaggttag cccttgggca tctgcccctg
     8881 gctggtggtg gtgtcggcgg cgggcgtcga attatcgatg gtttcgccat agaattccgc
     8941 ggccgcccag gcaatgacga tcagcacgag cgcgcaaatg agtatgagca ggacccgttt
     9001 gccccagccc gcctgccggg ctttctgctc tggaatgatt ttggtcattg tcggtctccc
     9061 gggttggtct ccttgctggg cctgctgggg gcattttgac cgcaaggcgt gtcggactgc
     9121 cggaggataa tggctccctt ccgtgaaagt tccgaatttc gaccggagcg tggagcgagc
     9181 cttgcgtcta ttgccgtccg gctttatcgg ggccgtcttt atccgaaccg tcttgcggct
     9241 tcgtgttttc gcggtagatc gcataggcgg cggcagcgac ggctggcgcg atgagggcgc
     9301 caacgccgcc cttgccccgc agcagcgccg gcaaaaccgc cagcgcggtg gtcacgccta
     9361 tcgcggtcat gtcggccttt cgccgccttg tctcccg
//
LOCUS       DASYCS010000379         2220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2571922,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000379 DASYCS010000000
VERSION     DASYCS010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2571922"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="VGM46_11875"
     CDS             complement(<1..795)
                     /locus_tag="VGM46_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017960601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HEY4193335.1"
                     /translation="MRTPSVVALLSVLCLAGCTSTSAIDDLQLQQPSNETTSSVVRPS
                     APIAAAQQVALSEPVEAFAEQSEPRELSPGPVPPMALADPDSVATRAARSITLVAPTK
                     PTVRPEPALARTIYGYGFRDAKPMNFGKASPRKLAVHGVDVSRWQGDIDWAKLHRQGA
                     NFVYIKATDGGDHLDPMFKKNWRTANEAGMKRGAYHFFYWCRTAGEQADWFIRNVPRV
                     EGALPPVIDVEWNHESSCKRRPSRKTVLEKMQVFMDRLERHYGQRPI"
     gene            complement(1274..>2220)
                     /locus_tag="VGM46_11880"
     CDS             complement(1274..>2220)
                     /locus_tag="VGM46_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY4193336.1"
                     /translation="LAASLGVELIRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTD
                     GVDQHPHCVLLLDEVEKAHPDLFNILLQVMDHGKLTDHNGKQIDFRNVILIMTTNAGA
                     SDAQRAAIGFGSTKREGDEVEAINRLFTPEFRNRLDAIIPFGSLPVPVIHQVVQKFVM
                     QLEAQLSERGVTFDLAPEAIAWLADKGYDERMGARPLGRVIQEHIKKPLADEVLFGKL
                     KKGGTVRITIEKKETGENGLKLETIADEARVSPKREPEEAPKSGKAGAKKPATKKAVA
                     QKPAAKGPAPKGPAPKSPASKGKDSGKRSLVPQLPRKG"
BASE COUNT          410 a          691 c          671 g          448 t
ORIGIN      
        1 gatcgggcgt tggccgtaat ggcgctctag ccggtccatg aagacctgca tcttttccag
       61 caccgtcttg cgcgacgggc ggcgtttgca cgacgattca tggttccact cgacgtctat
      121 caccggcggc agcgcgccct cgacgcgcgg cacgttgcgg atgaaccagt cagcctgctc
      181 gccggcagtg cggcaccagt agaagaagtg ataggcgccg cgcttcatgc cggcttcgtt
      241 ggcggtgcgc cagttcttct tgaacatcgg gtcgagatgg tcgccaccgt cagtcgcctt
      301 gatatagacg aagttcgcgc cctgccggtg cagcttcgcc cagtcgatgt cgccttgcca
      361 gcgcgaaacg tcgacgccgt gaacggcgag ttttcgaggc gaagccttgc cgaaattcat
      421 tggcttggca tcgcggaagc cataaccgta gatcgtgcgg gccagtgccg gctccggccg
      481 caccgtcggc ttggtcggcg cgaccagcgt gatcgacctg gcggccctgg tggcgacgct
      541 atcgggatcg gcaagcgcca tcggcggcac tgggccgggc gacaactccc gcggctcgct
      601 ttgctcggcg aaagcttcaa ccggttcgct taaggcgacc tgctgcgctg ccgctatcgg
      661 ggcgctcggc cgtaccaccg agctggtggt ttcgttcgac ggctgttgca attgcaggtc
      721 gtcgatggcg gacgtggatg tgcaaccagc caggcacaat accgaaagga gggcgacaac
      781 gcttggcgtt cgcatctgaa cctgtacgca aaacaaaaca gatcagcggc accatcaccg
      841 cccacaaagc cgaagcctaa ctcctaacaa gaaattacca ataaagtttg aatcgaatct
      901 ttaccatatc aggcagcgca aggcgggcag atgccgcctc ccccgccgtc ctgaacctat
      961 cctgtaacgc gaatggctgg acccgtcgcg cgttcgggga gatgtatata aatccgcagg
     1021 acaagaacga ggaatggcgg agcgaccatc acgcctgtgc caatgccgat gccgccggcg
     1081 cagaagcgat aaaaaagccc cgtttccggg gctttcagac taccgacaaa ccgcctcagg
     1141 tctacggaac ggcacagatt taaaccgtga acatatcgga atcaaaaccc ctcgatttcg
     1201 accgctgtca aaatcgaggg gtttgtgatc aatctgaaaa gcccgtttcc ggggctttcc
     1261 agtcgtattg gcatcatccc ttacggggca gttgcggcac cagactgcgc ttgccgctgt
     1321 ccttgccttt tgaggctggg cttttgggcg ccgggccctt gggcgcaggt cccttggcgg
     1381 cgggcttttg cgccacggcc ttcttcgtcg ccggcttttt cgcgccagcc ttgccggact
     1441 ttggcgcttc ttccggttcc cgcttggggc tgacccgcgc ttcgtcggca atcgtttcga
     1501 gcttcaggcc gttttcaccg gtttccttct tctcgatggt aatgcgcacc gtgccgccct
     1561 tcttcagctt gccaaacaga acctcgtcgg cgagcggctt cttgatgtgc tcctggatga
     1621 cacggccaag cggccgcgcg cccatgcgct cgtcatagcc cttgtcggcc agccaggcga
     1681 tggcttccgg cgcgagatcg aaggtaacgc cacgctcgga aagctgggct tccagctgca
     1741 tgacgaactt ctgcaccacc tggtggatga ccggaaccgg cagcgatccg aacgggatga
     1801 tggcatcgag gcggttgcga aactccggcg tgaacagccg gttgatcgcc tcgacttcat
     1861 cgccttcgcg cttggtcgaa ccgaaaccga tcgccgcgcg ctgcgcatcc gatgcgcccg
     1921 cattggtggt catgatcagg atgacattgc ggaagtcgat ctgcttgcca ttgtggtcgg
     1981 tcagcttgcc gtggtccatt acctgcaaca ggatgttgaa caggtccggg tgggcctttt
     2041 ccacttcgtc cagcagcagc acgcaatgcg gatgctggtc gacgccgtcg gtcaacaggc
     2101 cgccctggtc gaagccgaca tagccgggag gtgcgccaat cagccgcgag acggtgtggc
     2161 gctccatata ttccgacatg tcgaagcgga tcagctctac gccgagcgag gccgccagct
//
LOCUS       DASYCS010000380         5791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1557558,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000380 DASYCS010000000
VERSION     DASYCS010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 5791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5791
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1557558"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VGM46_11885"
     CDS             <1..275
                     /locus_tag="VGM46_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEY4193337.1"
                     /translation="LKLSEQAILERGYALVLDAAGALVKRAADIKPGEMLELRFADGA
                     AAAIAGGAAGDGQTGQKPQIDAKTQARATPKPAAKAREPGNQGSLF"
     gene            complement(325..597)
                     /locus_tag="VGM46_11890"
     CDS             complement(325..597)
                     /locus_tag="VGM46_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193338.1"
                     /translation="MSEHAIEFLQEWICEKVQAPAHPAMVEKHAATLARECAAKAAEA
                     GIPLEDIQEEVGDIQDLIAAKLEDAAEAENASGDGMDADNENEPAK"
     gene            complement(785..861)
                     /locus_tag="VGM46_11895"
     tRNA            complement(785..861)
                     /locus_tag="VGM46_11895"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(825..827),aa:His,seq:gtg)
     gene            complement(1223..1681)
                     /locus_tag="VGM46_11900"
     CDS             complement(1223..1681)
                     /locus_tag="VGM46_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin"
                     /protein_id="HEY4193339.1"
                     /translation="MNKSKTADTIENVEFPSFDSSRATDQIRAFAEKSVEQSKEAYSR
                     LKTGAEETQKVLESTYETARSASSDLSLKTIAALRTNADAGFSHLEALIGARSLSEVF
                     ELQSAFVRKQVEAGVQQAKDLQTVATKAAEDVSKPVKSAVEKAFAELKVA"
     gene            1928..5503
                     /locus_tag="VGM46_11905"
     CDS             1928..5503
                     /locus_tag="VGM46_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY4193340.1"
                     /translation="MPSDSYSFLDVAVLDPVRQRFAAGDALVILSIDLEEVIWTNGPG
                     AALFGYPDIEAIIGASAGLPPIARRQIMATGGYPEIGSDRAILVRLASGMTSHAVAFL
                     ASAVTMPDGGKAILLALPAIRSGAVGARETAAAIGGFTEGSHFVAFIDGSGNVEAASE
                     GFSRLGITAQTMAGLIADVAAERGRIVKRLVPGGKGSYPAGFARLTDDPARHLLVVID
                     EGRQGAEPQPRDETGTDAAQAPMATEPQQAAAPVSPEPALGNSGDAQPGPSALNDHWH
                     FSTESETGAPSQSPGHDAPDAAPNADAVSPEAAPGQTGTPAADSPAIEQPAAAPAIDR
                     LAAPVRFVWRTDADGNFSMVSPEFTAVVGQPAANIIGRHFRQVAADFGLDPSGEIAGL
                     LERRDTWSGRSVLWPVAGTSLAVPVDLAALPVYDRNRVFEGFRGFGVARGGDAVADAN
                     ATGLKLDKITTPPIPESPQEPADADPFKGEVPALAIAPQPERRFADKVIRLAEHRQPA
                     NDKGLSTLERSAFREIGERLKKENGPGTAASDAPAATPATPEATPPASPPAQDAAGAE
                     AEAGRQPLEQQAAPPAHDTAAEPDETKHDTGQTERAEGEPPIEASAKKPRSLFDFGEA
                     QPAGIGAPSVFEPARQDDSAATRHEPQATTEEASQPKPASAQQQLATPLKNGGYLPSA
                     FAIGAASAGAPDTSILAKLPVPLLFHAGDTLHYANEEFLDLTGYGSLGELAQAGGLDA
                     LFADPYAPENAPDNGDRMLKLKTRDGLEFPIEAILRSVPWGAGKALMLVVRRTGDDEP
                     QEAAATVSHEAGVQAQPDISDLKARIAEMRTIIDTATDGVVLIDRDGMIRSISRPAEA
                     LFGFDSDEVAGKPFASLFAIESQRAARDYLAGLSDNGVASVLNDGREVIGRESQGRFI
                     PMFMTIGGLPNGSGFCAVVRDITQWKRAEEDLTQARAVAERASSQKTDFLARISHEIR
                     TPLNAIIGFSELMVDEKFGPVANDRYRDYLRDINRSGNHVLDLVNDLLDISKIEAGQQ
                     EMDYEAVSLNDTLAETVAMMQPQANRERVIIRSSFASKLPEVVADLRSVRQIALNILS
                     NAIRYTQAGGQVILSTAYEPSGDVVMRVRDTGIGMSQAEIEQAMKPFKQINALKRGRS
                     DGTGLGLPLTKAMVEANRARFSINSTPGEGTLVEVAFPSTRVLAE"
BASE COUNT         1121 a         1840 c         1837 g          993 t
ORIGIN      
        1 cgctgaagct ttccgaacag gcgatcctcg aacgcggcta cgcgctggtg ctggacgcgg
       61 ccggggcgct ggtgaagcgc gcagccgaca taaagcctgg cgagatgctg gaactgcgct
      121 ttgccgacgg agcggctgcg gccattgccg ggggcgcagc gggcgatggg caaaccggcc
      181 aaaagccgca gatcgacgcc aagacgcagg cgcgcgctac gccaaagccc gccgccaagg
      241 cgcgggaacc cggcaaccag ggttcgctgt tttaaaaaag cgggagcagc cggccatgcc
      301 gtgccggcag gccaatagct gaacttattt agcaggctcg ttttcgttgt cggcatccat
      361 gccgtcaccg gaagcatttt cggcttcggc cgcatcctca agcttggcgg cgatgaggtc
      421 ttgaatgtcg ccgacctcct cctggatatc ctccagaggg atgcccgcct cagcggcctt
      481 ggccgcgcat tccctggcca gcgttgcggc gtgcttttca accatcgccg ggtgcgccgg
      541 agcctgaacc ttttcgcaga tccattcctg caagaattcg attgcgtgct cgctcatgcc
      601 cgaaaaacct caagccgatt cgtcatattc aacgcatgac ctcgccaaaa gatgcccgaa
      661 tcatttcgcc cgaatatctc cgtgccatca ggcgtaccat tggcgggcaa aggaaaggcg
      721 gttttgggaa aattcggaat tcaaaaggga aaatccaatc ctttatcggg ccctcatcgg
      781 gccttggtac cgttggctgg gctcgaacca gcgacctccg gatccacaat ccggcgctct
      841 aaccaactga gctacaacgg cacatatacc tggacatgat cgagcctgaa ccaattcctg
      901 ccggccatgc tccctcgtca ggcgttgcgt cattacgggc aatcgaactt taattcaagc
      961 catttgggaa ggcaaagcgc ctgtcgtgca ccactctctt ccaaacggcc ccggagtaaa
     1021 cggcagacaa agaaaaaggc cggcgggagg aggtgccggc ctttttccag tccatagcca
     1081 gccggaggag gcgctggctg ctttcccgat gggcggaggg aggaggaacc atccgccggt
     1141 attcaatagg accgccttca gataggagcg gtcgcaccgg aaagaatgca acgttttgtc
     1201 gcattcccgg ctaaattcgc aatcaggcga ccttgagttc ggcgaaggcc ttctcgacgg
     1261 cggacttgac cggcttggag acgtcttcgg cagccttggt ggcgacggtc tgcaagtcct
     1321 tggcctgctg gacgccggcc tcgacctgct tgcgaacgaa ggcgctctgg agttcgaaga
     1381 cttccgacag cgaacgggcg ccgatcaggg cttcgagatg cgagaagcca gcgtcggcat
     1441 tggtgcgcag cgcggcgatg gtcttgagcg acaggtcgct ggaagcgctc ctggcggttt
     1501 cataggtgga ttccagaacc ttctgggttt cctcggcgcc ggttttcagc ctggaatagg
     1561 cttctttcga ctgctcgacg ctcttttccg cgaaagcgcg gatctgatcg gtggccctgg
     1621 aggaatcaaa gctggggaat tcgacgtttt cgatggtgtc agcggtcttg gacttgttca
     1681 tcttcgcatc ctaacttggc atcggcggca tggagcacaa ccgcgcaatt catgtatgaa
     1741 ccgcatatag acctttccgt tgtgcattgc aacatttttg ttgcgctgca atatgacggc
     1801 attaaccacg cgcccagaaa tcgccggcct cccgctgtgg attgtggacg ttgcggcccg
     1861 cgacctttat taattaacaa agcgttaact gaaaaaccgc ccgcattgtg agggtacttt
     1921 cgaacccatg ccgtcagaca gctattcgtt tctcgatgtc gccgtgctcg atccggtacg
     1981 gcaacgcttc gccgccggcg acgcgttggt catcctttcg atcgatctgg aagaagtgat
     2041 ctggacgaat ggaccgggtg ccgcgctgtt cggctacccg gacatcgaag ccatcatcgg
     2101 cgcttcggcc ggcctgccgc caatcgcccg gcggcagatc atggcgaccg gcggctatcc
     2161 tgaaatcggc agcgaccgcg ccatactggt gcgcctggca agcggcatga ccagccatgc
     2221 ggtggctttc ctcgccagcg cggtgaccat gccggacggc ggcaaggcga tcctgctggc
     2281 cctgccggcg atccggtccg gcgcggtagg cgcgcgcgaa accgcagccg ccattggcgg
     2341 cttcacggaa ggcagccatt tcgtcgcctt catcgacggc tccggcaatg tggaagcggc
     2401 atcggaaggc ttctccaggc tggggatcac ggcgcagacg atggccgggc tgattgccga
     2461 cgtggcagcc gagcgtggcc gcatcgtcaa gcgcctcgtg cctggcggca aaggctccta
     2521 ccccgcaggg tttgcacggc tgacggacga tccggcgcgc catctgctgg tcgtcatcga
     2581 cgagggcaga cagggtgccg agccacagcc gagagacgag accgggacag atgccgcgca
     2641 agcgcccatg gccacggagc cgcagcaagc agcggctccc gtttcacccg aacccgcgtt
     2701 gggcaactcc ggcgatgcgc agccgggccc atccgcgctg aacgaccatt ggcatttcag
     2761 cacggagagc gagacaggcg cgcccagcca gtcgccggga catgacgcgc cggacgcagc
     2821 accgaatgcg gacgccgttt cgcctgaagc cgcgccggga cagaccggca cgccggcagc
     2881 ggacagcccc gcgatcgaac aacctgccgc cgcgcccgcg atcgatcgcc tggcagcccc
     2941 cgtgcgtttt gtctggcgca cggatgcgga cggcaatttc agcatggttt cgccggaatt
     3001 caccgccgtc gtggggcagc cggccgccaa catcatcggc cggcacttca ggcaagtcgc
     3061 agccgacttc ggtctcgatc cttctggcga gatcgccggc ctgcttgagc gccgcgacac
     3121 atggtcgggg cgttcggtgt tgtggccggt tgcgggaacg tcgctcgccg ttcccgtcga
     3181 tctggcggcg ttgcccgtct atgaccgcaa ccgcgttttc gaaggctttc gcggcttcgg
     3241 cgtcgcgcgc ggcggcgatg cggtcgccga tgcgaacgcc accgggctga aactggacaa
     3301 gatcaccacg ccaccgatac cggaaagccc gcaggaaccg gcggatgccg acccattcaa
     3361 gggcgaagtg ccggcgctgg ccatcgcgcc gcagccggaa cggcgctttg ccgacaaggt
     3421 aatccggctg gccgaacacc gccagccggc caatgacaag ggcctgtcga cgctggagcg
     3481 cagcgcgttt cgcgaaatcg gcgagcgttt gaagaaggaa aacggacccg gcacggctgc
     3541 ctccgatgcg cccgccgcaa cgccggccac gccggaggca acgccacccg cctccccgcc
     3601 ggcgcaggac gcggcagggg cagaggcaga ggcaggccgc cagcctttgg aacagcaggc
     3661 cgcgccgccg gcgcacgata ccgcagccga gccggacgag acaaagcacg ataccgggca
     3721 gacagaacgg gccgaaggcg agccaccgat cgaggcctcc gcgaaaaagc cgcgttcgct
     3781 gttcgatttc ggcgaagcgc agccagccgg gattggcgcg ccatccgttt ttgagccggc
     3841 gcgccaggac gatagcgcgg caacgcggca cgagccgcaa gccaccaccg aagaggcaag
     3901 ccagccgaag cccgcaagcg ctcagcagca acttgcgacg ccgctcaaaa atggcggcta
     3961 tctgccgtcc gccttcgcga tcggcgctgc gtccgccggc gctcccgata cgtcgatcct
     4021 cgccaagctt ccggtgccgc tgcttttcca tgcgggcgac acgctgcatt acgccaatga
     4081 agaattcctg gacctgaccg gctatggctc gcttggcgaa ctggcgcagg cgggcgggct
     4141 ggacgcgctg tttgccgacc cttatgcgcc ggaaaacgcc cccgacaatg gcgaccgcat
     4201 gttgaagctc aagacccgcg acgggctgga gttccccatc gaggccatct tgcgctcagt
     4261 gccgtggggc gcgggcaagg cgctgatgct ggtggtgcgg cgcaccggcg acgacgagcc
     4321 tcaggaggct gcggccactg tctcgcatga agctggcgtg caggcccagc cggatatttc
     4381 cgacctcaag gcgcgcatcg ccgaaatgcg caccatcatc gataccgcga ccgacggcgt
     4441 cgtgctgatc gaccgcgacg gcatgatccg gtccatcagc cggccggccg aagcgctgtt
     4501 cggcttcgac agcgacgagg tggccggcaa gccgttcgct tcgctgtttg ccatcgaaag
     4561 ccagcgcgcg gcgcgcgact acctggccgg gctttccgac aatggcgtgg ccagcgtgct
     4621 caacgacggc cgcgaggtta tcgggcgcga atcgcagggc cggttcattc cgatgttcat
     4681 gaccattggc gggctgccca atggaagcgg cttctgcgcg gtggtgcgcg acatcaccca
     4741 atggaagcgg gccgaggaag acttgacgca ggcccgcgcc gtggccgagc gcgcatcctc
     4801 gcaaaagacc gatttcctag cccgcatcag ccatgaaatc cgtacgccgc tgaacgccat
     4861 tatcggcttt tccgaactga tggtggacga gaaattcggg ccggtcgcca atgaccgcta
     4921 ccgcgattac ctgcgcgaca tcaatcgttc aggcaatcat gtgctggacc tggtcaacga
     4981 cctgctcgac atttccaaga tcgaggctgg ccagcaggag atggactatg aggcggtgtc
     5041 gctgaacgac acgctggccg agacggtggc gatgatgcag ccgcaggcca accgcgagcg
     5101 cgtcatcatc cgttcgtcct tcgcttccaa gctgccggaa gtggtggccg acctgcgcag
     5161 cgtgcgccag atcgcgctca acatcctgtc caacgccatc cgctacacgc aggccggcgg
     5221 ccaggtgatc ctttcgacgg cctacgagcc ttcgggcgac gtggtgatgc gcgtgcgcga
     5281 taccggcatc ggcatgagcc aggccgagat cgagcaggcg atgaagccgt tcaagcagat
     5341 caacgcgctg aagcgcgggc gcagcgatgg gaccggcctt ggcctgcccc tgaccaaggc
     5401 gatggtggag gccaaccgcg cgcgcttctc catcaactcg acgcccggcg aaggcacgct
     5461 cgtggaagtc gcgttccctt cgacgcgggt gctggcggaa tagagcattg ccagcaaaag
     5521 tgtgaaacgg ttttgcgtcc ggaaatgccg tcaaaaaaga gatggagcgg tttcgtcgat
     5581 tcaataaaaa tcggaaccgc tccaagcgtt atttgcgccc aaaaaaaagg cgcccgatgg
     5641 acgcctttag atgatgagat tggtcacaac gggcttgagt acgaatgtcc tcacgggaga
     5701 acggggatac cctcaagttg gttgcgacta tcggcggcgg gacttaacaa atccttaccg
     5761 ggcgcgcgga aactttacga atccgtaccc t
//
LOCUS       DASYCS010000381         4258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1992836,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000381 DASYCS010000000
VERSION     DASYCS010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4258
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1992836"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..856
                     /locus_tag="VGM46_11910"
     CDS             59..856
                     /locus_tag="VGM46_11910"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEY4193341.1"
                     /translation="MARSAILNVMVQAAMKAGRSLSRDFGEVQNLQVSMKGPGDYVSQ
                     ADRKAEDIIFAELSKARPGYGFLMEERGAVAGDDDQHRWIVDPLDGTTNFLHGIPLFA
                     VSIALERQGQLVAGVIYNPAMDELYTAERGGGAFMNDRRLRVAARSKLADCVIGCGVP
                     HLGRGQHGNFLVELRNVMAEVSGIRRMGAASLDLAYVAAGRMDGFWETGLSAWDIAAG
                     ILLIREAGGFLSDAHGGQGMLDSGSVIAGNEVIRQVLEKTLKRPIKG"
     gene            1000..1650
                     /locus_tag="VGM46_11915"
     CDS             1000..1650
                     /locus_tag="VGM46_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEY4193342.1"
                     /translation="MGRWHYSRAEKIADGVVHAVGIVLAAIAGAMLLAYAASRSYPGE
                     YIAAVFYVVSLLTVLSVSLAYNLWPVSSPAKWVLRRVDHSAIYLLIAATYTPFLAQIE
                     GSPLARLMIVTVWAAAAMGIALKIFLPGRFDRLAVLFYLAIGWSGVILAEPLAQALPA
                     NSVRLIIAGGIVYSLGVVFFVWKRLRFHTALWHGFVVTGAGLHLAALADCLVLNRL"
     gene            1769..2794
                     /locus_tag="VGM46_11920"
     CDS             1769..2794
                     /locus_tag="VGM46_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006200136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEY4193343.1"
                     /translation="MAFLRSFGIGRRSDVLDYDPHRLSSPQVFLLSMVIFLVIVAFIA
                     AILTRQISTAFVHNPGLNGLIVGVLIVGIILAFGQVVRLFREVRWVNSFRAGSETVEP
                     VLLAPMKAMIGRSSSVAFSTSSMRTMLDSIATRLDESRDTSRYLVGLLIFLGLLGTFW
                     GLLGTIGSIGDTIEALDPGTGDSAAVLEALKAGLAAPLAGMGTAFSSSLFGLSGSLVL
                     GFLDVQAGRAQTRFYTELENWLSSVTDLSSDLVVADTAKTESSDEIRALSERLRSMQE
                     AGGSNPRVATAMANLADGISGLVKNMRSEQQIMRDWVEAQSDEQKAMRNTLEKIAEAL
                     KKQQGKN"
     gene            2795..3826
                     /locus_tag="VGM46_11925"
     CDS             2795..3826
                     /locus_tag="VGM46_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004689214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan -binding protein"
                     /protein_id="HEY4193344.1"
                     /translation="MALARGRRGDRHIDYWPGFVDALSTLLLAIMFLLSVFVLAQFLL
                     SREISGKDAVLNRLNSQINELTSLLALEKGNTQDAQDALANIQASLSASEAEKSRLEQ
                     LLAQGTGAGDAANARANTLTGELDAQRQLSQRAQSQVELLNQQIAALRKQIGALEDAL
                     DASETRDRDSNTKIADLGKRLNVALAQRVQELNRYRSDFFGRLREILSDRENIRIVGD
                     RFVFQSEVLFPTGSEQINDAGKVEMKKLADAIIELQKEIPPEINWVLRVDGHTDNVPL
                     SGAGRYRDNWELSSARAVSVVKFLIANGVPANRLVAAGFGEFQPLDPADTEEARNKNR
                     RIELKLTER"
     gene            4050..>4258
                     /locus_tag="VGM46_11930"
     CDS             4050..>4258
                     /locus_tag="VGM46_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193345.1"
                     /translation="MRSFTLGTSVKMIAVAGALLAVPLAGASAAQFNDHTGRMATPTP
                     TSFQGPRADSILNQVRGIDQGIADA"
BASE COUNT          733 a         1351 c         1358 g          816 t
ORIGIN      
        1 acgaaatcac ctacattcgc cgcgccgatt gatcgcgcag gcaggaagct agagaaaaat
       61 ggcgcgttcc gcgatcctca acgtcatggt ccaggccgcc atgaaggccg gccgctcgct
      121 gtcgcgcgac ttcggcgaag tgcagaactt gcaggtctcg atgaaggggc cgggcgatta
      181 tgtcagccag gccgaccgca aggccgagga catcatcttc gccgaattgt cgaaagcgcg
      241 gcccggctac ggcttcctga tggaagagcg tggcgcggtt gccggcgatg acgaccagca
      301 tcgctggatc gtcgatccgc tcgacggcac caccaatttc ctgcacggca tcccgctgtt
      361 tgccgtctcg atcgcgctgg agcggcaggg ccagctggtc gccggcgtca tctacaatcc
      421 ggcgatggac gagctttaca ccgccgagcg cggcggcggc gccttcatga acgaccgccg
      481 cctgcgcgtc gcggcgcggt ccaagcttgc cgattgcgtg atcggctgcg gcgtgccgca
      541 tctcggtcgc ggccagcatg gcaatttcct ggtcgaactg cgcaatgtga tggccgaggt
      601 ttccggcatc cgccgcatgg gcgcggcctc gctcgatctc gcttatgtcg cggcggggcg
      661 catggacggt ttctgggaaa ccgggctgtc ggcatgggac atcgcggccg gcatcctgct
      721 gatccgcgag gcgggcggct tcctgtccga cgcccatggc ggccagggca tgctcgacag
      781 cggctcggtc atcgccggca acgaggtgat ccggcaggtg ctggaaaaga cgctgaagcg
      841 gcccatcaag ggataggctg caccttctta agtctggccg tttcgtcata aagctgcgat
      901 gcaagtctgg catgaagcct gcatggtccc caaatgaagg gacgacaggc ttgccggatg
      961 accccaactg acacaccaag cggacagaca gttcccttca tgggacggtg gcactattcg
     1021 cgcgccgaga aaatcgccga tggcgtcgtc cacgcggtcg gcatcgtcct ggcggcgata
     1081 gcgggcgcga tgctgctggc ttacgccgct tcgcgatcct atcccggcga gtatatcgcg
     1141 gcggtgtttt atgtggtgtc attgctgacc gtgctgtcgg tgtcgctggc ctataatctg
     1201 tggccggttt cctcgccggc gaaatgggtg ctgcggcggg tcgatcattc ggcgatctac
     1261 cttcttatcg ccgctaccta cacgcccttc ctcgcccaga tcgaaggctc gccgctggcg
     1321 cggctgatga tcgtcacggt gtgggcggcg gcggcaatgg gcatcgcgct gaagattttc
     1381 ctgcctggcc gtttcgatcg gctggccgtg ctgttttatc tcgccatcgg ctggagcggc
     1441 gtgatcctgg ccgaaccgct ggcgcaggcg ctgccggcca attcggtccg cctcatcatc
     1501 gccggtggca tcgtctattc gctgggcgtc gtctttttcg tgtggaaacg ccttcgcttc
     1561 cacacggcgc tgtggcatgg cttcgtcgtc accggggccg ggctgcatct ggcggcgctg
     1621 gccgattgcc tggtgctcaa ccgtctttga cggccgatgt gatcgggcat ggctcttgcc
     1681 aaaggccttt caattcagtc acattcccgc ttagactccg acccggcctc acgggcggca
     1741 tcagacgcgg catcggaacg gaacacggat ggcatttctc agatcgttcg gaattggcag
     1801 gcgctcggat gtgctcgact acgatccgca caggctgtcc agcccgcagg tgttcctgct
     1861 gtcgatggtc atcttcctcg tcatcgtcgc cttcatcgcg gccatcctga cgcggcaaat
     1921 ttccaccgcc ttcgttcaca atcccggcct caacgggctg atcgtcggcg tgctgatcgt
     1981 cggcatcatc ctcgccttcg ggcaggtcgt gcggctgttt cgcgaggtgc gctgggtcaa
     2041 ttcctttcgc gccggctccg agaccgtcga gcctgtgctg ctggcgccga tgaaggcaat
     2101 gatcgggcgc tcttcctcgg tcgccttttc gaccagctcg atgcgtacca tgctcgattc
     2161 catcgccacc cgcctcgatg aaagccgcga tacttcgcgc tatctggtcg ggctgctgat
     2221 cttccttggc ctgctcggca cgttctgggg cctgcttggc accatcggct ccattggcga
     2281 caccatcgag gcgctcgatc ccggcaccgg cgattccgcc gcggtgctcg aagcgctgaa
     2341 ggcggggttg gccgcaccgc ttgccggcat gggcacggcg ttttcttcct cgctgtttgg
     2401 cctttccggc tcgctggtgc tcggctttct cgacgtgcag gccgggcggg cgcagacgcg
     2461 cttctatacc gaactcgaaa actggctgtc gtcggtgacc gacctgtcgt cggacctcgt
     2521 cgttgccgac acggccaaga ccgagtcgtc cgacgagata cgcgccctgt cggaacggct
     2581 gcgttccatg caggaggcgg gcggctccaa cccgcgcgtg gcgaccgcca tggccaatct
     2641 tgccgacggc atttcggggc tggtcaagaa catgcgctcc gaacagcaga tcatgcgcga
     2701 ctgggtggag gcgcagtcgg acgaacaaaa ggcgatgcgc aacacgctgg agaagatcgc
     2761 cgaagcgctg aagaagcagc aggggaagaa ctaggtggcg ctggcgagag ggcggcgcgg
     2821 cgaccgccac atcgattact ggccgggctt cgtcgatgcg ctgtcgacgc tgcttttggc
     2881 catcatgttc ctgctttcgg tgttcgtgct ggcgcaattc ctgctcagcc gcgaaatctc
     2941 cggcaaggac gccgtgctca accggctcaa ctcgcagatc aacgaactga cctcgctgct
     3001 ggcgctggaa aagggcaaca cgcaggatgc gcaggatgcg ctggccaaca tccaggcatc
     3061 gctgtcggcc agcgaggcag aaaagagccg ccttgagcag cttttggcgc aagggacggg
     3121 cgccggcgat gccgccaatg cacgcgccaa cacgcttaca ggcgaactgg acgcgcagcg
     3181 ccagctaagc cagcgcgcgc agtcgcaggt ggaattgctg aaccagcaga tcgcggcgct
     3241 gcgcaagcag atcggcgcgc tggaagacgc gctcgatgct tcggagacgc gcgaccgcga
     3301 ttccaacacc aagatcgccg atctcggcaa gcgccttaac gtcgcgctgg cgcagcgcgt
     3361 gcaggaactg aaccgctacc gttcggattt cttcgggcgg ctgcgcgaaa tcctttccga
     3421 ccgcgaaaac atccgcatcg tcggcgaccg cttcgtcttc cagtcggagg tgctgtttcc
     3481 gaccggctcc gagcagatca acgatgccgg caaggtcgaa atgaagaagc tggccgacgc
     3541 gatcatcgag ctgcaaaagg aaattccgcc ggagatcaac tgggtgctgc gcgtcgatgg
     3601 ccacaccgac aacgtgccgc tgtcgggggc cgggcgctat cgcgacaatt gggaattgtc
     3661 gtcggcgcgc gccgtatcgg tcgtcaagtt cctgattgcc aacggcgtgc cggccaatcg
     3721 gttggtggcg gccggtttcg gcgaattcca acctctcgat ccggccgaca ccgaggaagc
     3781 ccgcaacaag aaccgccgca tcgagctaaa actcaccgag cggtgacggc ttttctccgg
     3841 cgggctgcac acgaaaggca cacggcaaag tgagccgggt tacctttttg taattacact
     3901 ttgccggtgc agcttcgtaa tttccatgac gcggaccgag acccgccggc ggcatcttcc
     3961 aatgctgtga cgcgacgaga ctggacggtc cgtggccgga agatacaacg cgaccaggcg
     4021 ttggcttcct catcaaagga gacgctgcca tgcgctcttt cacacttgga acgtccgtaa
     4081 aaatgatcgc tgttgccggc gccttgctgg ccgtgccact ggccggcgcg tcggcagcgc
     4141 agttcaacga ccacaccggc agaatggcga cgccgacacc gacttccttc cagggcccgc
     4201 gcgccgacag catcctgaac caggtgcgcg gtatcgacca gggtatcgcg gatgccag
//
LOCUS       DASYCS010000382         1649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4020957,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000382 DASYCS010000000
VERSION     DASYCS010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4020957"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1100)
                     /gene="hemW"
                     /locus_tag="VGM46_11935"
     CDS             complement(<1..1100)
                     /gene="hemW"
                     /locus_tag="VGM46_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006326436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEY4193346.1"
                     /translation="MMMKLDRTPGFGVYIHWPFCAAKCPYCDFNSHVRHQPVDQERFA
                     RAFEAELATMRARTGPREVTSIFLGGGTPSLMKPETVGAVLDAVAKNWSVPDGIEVTL
                     EANPSSVEAERFRGYRAAGVNRVSLGVQALNDTDLRFLGRLHNVSEALRAIGLAREIF
                     PRLSFDLIYARPGQTPEAWAAELEQAIGHAADHLSLYQLTIEEGTPFHALHRAKKFAI
                     PDDDHAAELYEITQAITTGRGLPAYEISNHARPGAESRHNLTYWRYGEYVGVGPGAHG
                     RFVENGRRVVTIAERMPETWASLVEAKGHGVVDGEVLSRGEEADEFLLMGLRLVEGID
                     LARYEQFSGRGLSSTRLSTLQDEGLVSPIGNSR"
     gene            complement(1097..>1649)
                     /locus_tag="VGM46_11940"
     CDS             complement(1097..>1649)
                     /locus_tag="VGM46_11940"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006326435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HEY4193347.1"
                     /translation="EYGLPEPDETGTTFEENAYIKALAAAKATGLPALSDDSGLCVDA
                     LDGAPGVYTANWAETPDGARDFTMAMQRTEVAMQEVGAKEPGQRTGRFVAVICLAFPD
                     GEAEYYRGEVAGTLVWPPRGEVGFGYDPVFLPDGYEKTFGEMSAQEKHGWKPGDRQAL
                     SHRARAFQKFAQARLDAARAGAE"
BASE COUNT          278 a          562 c          526 g          283 t
ORIGIN      
        1 cgcgaattgc cgatgggcga aaccagcccc tcgtcttgaa gcgtcgaaag ccgcgtcgaa
       61 gacaggccgc gtccggaaaa ctgctcgtag cgggcaaggt cgatgccctc caccagccgc
      121 agacccatca acaggaattc gtcggcttcc tcgccgcgcg acaggacctc gccatcgacc
      181 acgccgtggc ccttggcctc gaccaggctg gcccaggttt ccggcatccg ttccgcgatc
      241 gttaccacgc gccggccatt ctccacgaaa cgtccatgcg cgccggggcc gacgccgaca
      301 tattcgccgt agcgccaata ggtcagattg tggcggcttt ccgcgcccgg ccgcgcgtgg
      361 ttggaaatct cataggccgg caggccgcgc ccagtcgtga tggcttgggt aatttcataa
      421 agctcggcgg catggtcgtc gtcgggaatg gcgaatttct tcgcccggtg cagcgcgtgg
      481 aagggcgtgc cttcctcgat ggtcagctgg tagagcgaca aatggtcggc ggcatggccg
      541 atggcctgct caagctccgc cgcccacgcc tccggcgtct ggcccggccg cgcgtagatg
      601 aggtcgaagg aaaggcgcgg aaatatctcg cgcgccagcc cgatggcgcg caacgcctcg
      661 gaaacattgt gcagccggcc gagaaaacgc aggtcggtgt cgttcagcgc ctgcacgccg
      721 agcgaaaccc ggttgacgcc ggcagcgcgg tagccgcgaa aacgctccgc ctcgacggag
      781 gaagggttgg cttccagcgt cacttcgatg ccgtccggca cgctccagtt cttcgccacc
      841 gcatccagca ccgcgccgac tgtctccggc ttcatcagcg aaggcgtgcc gccgccgagg
      901 aaaatcgagg tcacctcgcg cggcccggtg cgggcgcgca tggtggcaag ttccgcctcg
      961 aaggcgcggg cgaaccgctc ctgatccacg ggttgatggc ggacatggct gttgaaatcg
     1021 caatagggac acttggccgc gcagaacggc cagtggatat agaccccgaa accgggtgtc
     1081 cggtcgagct tcatcatcat tccgcgccgg cccttgccgc atccagccgt gcctgcgcga
     1141 atttctggaa agcccgcgcc cgatgcgaca gcgcctgccg gtcgccgggt ttccagccgt
     1201 gtttttcctg cgcgctcatc tcgccaaatg tcttttcata gccgtcgggc aggaagaccg
     1261 gatcatagcc gaagccgact tcgccacgcg gcggccacac cagcgtgccg gcaacctcgc
     1321 cgcggtaata ttcggcctcg ccatccggaa aggcaaggca gatgacggcg acgaagcggc
     1381 ctgtgcgctg gcctggctct ttcgcgccca cctcctgcat cgccacttcg gtgcgctgca
     1441 tggccatggt gaagtcgcgc gcgccatcgg gcgtttctgc ccagttggca gtgtagacgc
     1501 ccggcgcgcc gtccagcgca tcgacgcaca ggccggaatc atccgaaagc gccggcaggc
     1561 cggtcgcctt ggcggctgcc aacgccttga tgtaggcgtt ttcctcgaag gtcgttccgg
     1621 tctcgtccgg ctcgggcagg ccgtattcc
//
LOCUS       DASYCS010000383         3151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_689430,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000383 DASYCS010000000
VERSION     DASYCS010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689430"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            259..729
                     /locus_tag="VGM46_11945"
     CDS             259..729
                     /locus_tag="VGM46_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HEY4193348.1"
                     /translation="MFVAASLISASGAAVADEVGKVGVDWLGNDIVIEAIKDPKVEGV
                     TCHVSYFDRGIIDRLHKGNWFEDPSDSSISCRQTGPITIGDIDLGEKGEEVFKQGISL
                     IWKKQVVNRIYDKANDTLIYLSHSRQVQNGSAKMSLTTVPLYGQSVVWSKGKPQ"
     gene            771..1409
                     /locus_tag="VGM46_11950"
     CDS             771..1409
                     /locus_tag="VGM46_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006336156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HEY4193349.1"
                     /translation="MERPEHLVAKDSEPRIHPTAELKACKLGRYASIGERVILREVAV
                     GDFSYFERHAEAIYTSIGKFCSIAANSRINALEHPMERLTTHKVSYRPNEYFRFLGVD
                     AAFRERRQGKPVTIGHDVWVGHGAVVMPGVDIGNGAVIGANAVVTRNVPAYTIVTGVP
                     AKPLRRRFPEAIAARIEALGWWDWPAEKLAKAIPDMQALPIEAFLDRWGAAL"
     gene            1600..1926
                     /locus_tag="VGM46_11955"
     CDS             1600..1926
                     /locus_tag="VGM46_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013530976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193350.1"
                     /translation="MNKFAIVLSIAAVSFYTVSAQAGGFGGKTKIVGGNSGVSSGLIN
                     VSPSIGLGNVTALNNILSGNGILSGNVVSGILNGNKTNAVTGILSGIGLTGIGGNSYK
                     LDSKHR"
     gene            complement(2006..>3151)
                     /locus_tag="VGM46_11960"
     CDS             complement(2006..>3151)
                     /locus_tag="VGM46_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193351.1"
                     /translation="ASLCLLCGMASAHAEPGGGVSASSSLERHWTSNALDSDHAVPDW
                     YTLLRGSLQQQFGDDDANIGLGAEFQATRHDRVSIEDDRALALSAHAFRRFSPSLELR
                     GTLTYRVSREGDDLPIGAFTLGTSTPKQILGASGQMGIDLGNATSLILDVADTYEKVG
                     ETRFGNELLLPAQLDPDLNRFQLGAHLTRTFGAIALGVSASALWMAVQELGSPPVALS
                     LAQYTLRGELTYTGADGSTLGAAFGGEFLRGANDTYSRLRPTWQITFARQLPHDLELR
                     GTYFGRFETLDSDDPLASWLQRGELELGMKLRENLAFASGLFAEEKENLLFENVERKR
                     GLYAELTCDATPSMAIVLRVDVSKTFKTVIEERESTVDTFVGLRAKI"
BASE COUNT          663 a          914 c          977 g          597 t
ORIGIN      
        1 accatcaaca ccagaatgcc gaccaggata gaacgcatca tcagaaaatt ccgcctcgtt
       61 tggaccgcgc cttcggtgtg gccggcgcga ccatatcgtt tacggcaagg ccaggcaaag
      121 cgccgcgttg ccgcaagggg cgaggtagtg cgcttgccgc aggcttgcaa tgcggcgcag
      181 ctgctcccag cttagggctg acggcatcgt gatttggaga cgttgttgct gaagcggatc
      241 ggaacgaagt gggcaggtat gtttgtcgca gcgtcgctca taagcgcgtc gggtgccgcc
      301 gtggccgatg aagtcggcaa ggttggcgtc gactggttgg gcaacgatat cgtcatcgag
      361 gcgatcaagg acccgaaggt cgagggcgtt acctgccacg tttcctattt cgaccgcggc
      421 atcatcgacc ggctgcacaa gggcaactgg ttcgaggacc cttccgattc gtccatttca
      481 tgccgccaga ccgggccgat caccatcggc gacatcgatc ttggcgaaaa gggcgaggaa
      541 gtgttcaagc agggcatcag cctgatctgg aagaaacagg tggtgaaccg catctacgac
      601 aaggccaacg atacgcttat ctatctgtcg cattcgcgcc aggtgcagaa cggatcggcc
      661 aaaatgtcgt tgaccaccgt gccgctttat ggccagagcg tcgtctggag caagggcaag
      721 ccgcaatagg cgcgcttgcg gtgggacgag cggcgggcta aatccgccgc atggaacgcc
      781 ccgaacatct cgttgcaaaa gattccgagc cgcgcatcca tccgacggcg gagttgaagg
      841 cctgcaagct cggccgttac gcctcgattg gcgagcgggt catcctgcgc gaggtcgcgg
      901 tcggcgattt ttcctatttc gaacgccacg ccgaggcgat ctatacgagc atcggcaaat
      961 tctgctccat tgccgccaac agccgcatca acgcgctgga gcatccgatg gagcggctga
     1021 ccacgcacaa ggtcagctat cggccaaacg agtatttccg cttcctcggc gtcgatgcgg
     1081 ccttccgcga gcgccggcag ggcaagcctg tcaccatcgg tcatgatgtg tgggtcggcc
     1141 atggcgcggt ggtgatgccg ggcgtggata tcggcaacgg cgcggtcatc ggcgccaatg
     1201 cggtcgtgac ccgcaacgtg ccggcctata cgatcgtcac cggcgtaccg gcaaagccgt
     1261 tgcgccggcg ttttcccgaa gcgatagcgg caaggatcga ggcgcttggg tggtgggact
     1321 ggccggcgga aaaactggcg aaggccatcc ccgacatgca ggcgctgccc atcgaggcgt
     1381 ttctcgaccg ctggggagcc gcgctctaac tcaacttcag gccatgctgg taaccaagtc
     1441 ccttaacgct aaagattagc gttaagaatt ggaaaacagg ttgtattatc gattttcatc
     1501 aatccagaat tgcaattcaa ttccgaaaat gtacaaatga ccttggaggt agaccacggg
     1561 agacagaagg aaacactgtc agcaacatag gagaagatca tgaacaagtt cgcaattgta
     1621 ctctctatcg cagctgtttc tttctatact gtatccgccc aggcaggtgg cttcggcgga
     1681 aaaaccaaga tcgtcggtgg caacagcggc gtttcgagcg ggttgatcaa tgtttcgccg
     1741 tcgatcggtc tcggcaatgt cacggcgctg aacaatatcc tcagcggcaa cggcatcctc
     1801 agcggcaatg tcgtttcggg tatcctgaac ggcaacaaga cgaatgccgt cacgggcatc
     1861 ctcagcggca tcggccttac cggcattggc ggcaacagct acaagctgga cagcaagcac
     1921 cgctaagcat cgactttgat tgcttacgac aaggacggga gggtgtttgc gcgccctccc
     1981 gtctgccgtt gtcgatcctg tcgaactata ttttcgcgcg cagcccgaca aaagtatcga
     2041 cggtgctttc gcgctcttcg atgacggtct tgaacgtttt cgaaacgtcg accctgagga
     2101 caatggccat cgacggcgtg gcgtcgcagg tcagttcggc gtagaggccg cgcttgcgct
     2161 cgacattttc gaaaagcagg ttttcttttt cttcggcaaa caggcccgat gcgaaggcca
     2221 ggttttcgcg gagcttcatg ccaagttcca actccccgcg ctgaagccac gaggcgaggg
     2281 gatcgtcgct gtccagcgtc tcgaaacggc cgaaataggt gccgcgcaat tcgagatcgt
     2341 gcggcaactg cctggcgaat gtgatctgcc aagtcgggcg caggcgactg taagtgtcgt
     2401 tggcgccgcg cagaaattca ccgccgaacg ccgcgcccag cgtcgagccg tccgcgccgg
     2461 tataggtaag ctcgccgcgc aaagtatact gcgccaaaga cagcgctacg ggcggggagc
     2521 cgagttcctg caccgccatc cacaatgccg acgcagatac gccaagcgcg atcgcgccaa
     2581 aggttcgggt caaatgcgcg ccaagctgga agcggttgag gtcggggtcg agctgcgccg
     2641 gcagcaaaag ctcgttgccg aagcgggttt cgcccacctt ttcataagtg tcggcaacat
     2701 cgaggatgag cgacgtggcg ttgccaaggt cgatccccat ttggcccgat gcgcccagga
     2761 tttgctttgg cgtgctggtg ccgagcgtaa atgcgccgat gggcaggtcg tcgccctcgc
     2821 gcgatacgcg ataggtcaac gtgccgcgaa gttcgaggct gggcgaaaag cggcggaagg
     2881 catgcgccga caaggcaagc gcacggtcgt cctcaatcga aaccctgtca tggcgcgtcg
     2941 cctggaactc ggcaccaagg ccgatattgg cgtcgtcgtc gccaaactgc tgttgcagcg
     3001 aaccgcgcaa cagcgtgtac cagtcgggga cggcatggtc gctgtccagc gcgttgctcg
     3061 tccagtgccg ctccaggctc gaagaggcgg aaacaccgcc gcccggctcg gcatgagcgg
     3121 atgccatgcc gcataacagg cacaggcttg c
//
LOCUS       DASYCS010000384         3350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3877500,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000384 DASYCS010000000
VERSION     DASYCS010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3877500"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..2590
                     /locus_tag="VGM46_11965"
     CDS             146..2590
                     /locus_tag="VGM46_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019857058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HEY4193352.1"
                     /translation="MLKPGLTNSDPETLAGEVLKALKYRVGKDTTVATPHDWLTASIV
                     VVRDRIVDHWIEATKEAYDHRKKRVYYLSLEFLIGRLMRDAFSNLGLMEDMRAALSSL
                     NVDLDVIAALEPDAALGNGGLGRLAACFMESMASVDIPAHGYGIRYANGMFRQEIQDG
                     WQVELPETWLDHGDPWEFERRERSFEVGFGGSVESVTAADGHLERHVWKPGERMLAVA
                     YDTPVVGWRANRVNTLRLWSGMPIDPIRLSAFNAGDHIGALAESNKADALSRVLYPAD
                     SHKEGQELRFRQEYFFSTASLQDIIQRHLSEYGDLTSLPDKAAIHLNDTHPAIAVPEL
                     MRLLMDVHGMDFDQAWSIAKRTFGYTNHTLLPEALESWPVPLFERLLPRHMQIVYAIN
                     AQVLLEARAANRFADDQISRISLIQENGDRRVRMGNLAFVGSHSINGVSALHTELMKE
                     TVFADLHRLYPDRINNKTNGITPRRWLIQCNPGLTELARQTIGDDFLDDIEAIRGLEA
                     YAGDAGFREKFAAVKRQNKVRLANLVAERLGIKVDPSALFDIQIKRIHEYKRQLLNIL
                     EAVALYDQIRSHPEREWTPRVKFFGGKAAPSYHNAKLIIKLANDVARVINSDPAVHGL
                     LKIVFVPNYNVSLAEIMMPAADLSEQISTAGMEASGTGNMKFALNGALTIGTLDGANV
                     EIKERVGDDNIFIFGLTTQEVAERRSNGYQPRAVIEGSPELSQALAAISSGVFSHDDP
                     TRYRELIDGLYNSDWFMVAADFDAYAATQRTVDAVWRDSPDWQARAIRNVARVAWFSS
                     DRTIREYAKEIWNVSA"
     gene            2647..>3350
                     /locus_tag="VGM46_11970"
     CDS             2647..>3350
                     /locus_tag="VGM46_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006330869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HEY4193353.1"
                     /translation="MRKPRATAASAEPEERAAASDVAAIVAGTHGDPFAVLGVQQAGG
                     AFVARCFVPGAQAVTACTLAGKAVGELARTNDAGFFEGRISIRKRQPLRYHAKNAGGD
                     WWVADPYSFGPVLGPMDDYYIAEGSHLRLFDKLGAHLIDHEGASGVQFAVWAPNARRV
                     SVVGDFNEWDGRRHPMRDRRDTGIWELFIPDIGAGRAYKYEILGADGVRLPLKADPFA
                     FKSELRPATASVTAEPP"
BASE COUNT          644 a         1079 c         1030 g          597 t
ORIGIN      
        1 cgtttctttg ctgatcaatt taaatcgatt gaagaaatgg ccgggccagc tagcctgagg
       61 ccgggatgac ggatagatca aaggttcgcc gccgctcggg cgacgaacag taaaaaagcg
      121 gaacgaacgc gtggaaagct ccgaaatgct caaacccggc ctcaccaatt ccgacccgga
      181 gacgctggcg ggcgaagtgc tcaaggcgct caaataccgg gtcggcaagg acaccaccgt
      241 cgccaccccg catgactggt tgaccgcatc catcgtggtg gtgcgcgacc gcatcgtcga
      301 tcactggatc gaggcgacca aggaagctta cgatcaccgc aagaagcggg tttactatct
      361 gtcgctggaa ttcctcatcg ggcggctgat gcgcgatgcc ttctccaatc tcggcctgat
      421 ggaggacatg cgcgccgcgc tgtcgtcgct gaatgtcgat ctcgacgtga tcgcggcgct
      481 tgaaccggac gccgccctcg gcaatggcgg tctcggccgt cttgccgcct gcttcatgga
      541 atccatggcc agcgtcgaca ttccagccca cggctatggc atccgctatg ccaacggcat
      601 gttccggcag gaaatccagg atggctggca ggtcgaattg cccgaaacct ggctcgacca
      661 tggcgacccg tgggaattcg agcggcgcga gcgttccttc gaagtcggct tcggcggctc
      721 cgtcgaatcg gtgaccgcag cggatggcca cctggaacgc catgtctgga agcccggcga
      781 gcggatgctg gcggtggcct acgacacgcc ggtggtcggt tggcgcgcca accgcgtcaa
      841 tacgctgcgg ctgtggtcgg gcatgcccat cgacccgatc cgcctgtctg ccttcaacgc
      901 cggcgaccac attggcgcac tggcggaaag caacaaggcc gatgcgctgt cgcgcgtgct
      961 ctaccccgcg gattcccaca aggaagggca ggagcttcgc ttccggcagg aatatttctt
     1021 ctccaccgcc tcgttgcagg acattattca gcgtcatctc agcgagtatg gcgatctgac
     1081 ctcgctgccc gacaaggcgg ccatccacct caacgacacc catccggcca tcgccgttcc
     1141 cgaattgatg cggcttttga tggatgtcca cggcatggat ttcgatcagg cctggagcat
     1201 agccaagcgc accttcggct acaccaacca cacgctgttg cccgaggcgc tggaaagctg
     1261 gccggtgccg ctgttcgagc ggctgttgcc gcgccacatg cagatcgtct acgccatcaa
     1321 cgcgcaggtg cttctggagg cacgcgcggc aaaccgcttt gccgacgacc agatcagccg
     1381 catttcgctc attcaggaaa acggcgaccg ccgcgtgcgc atgggcaacc tcgccttcgt
     1441 cggctcgcat tcgatcaacg gcgtctccgc gctccatacc gagttgatga aggaaacggt
     1501 gtttgccgac cttcaccggc tctatcctga ccgcatcaac aacaagacca acggcatcac
     1561 gccgcgccgc tggctgatcc agtgcaatcc gggcctgacc gaactggcgc gccagacgat
     1621 cggcgacgat ttcctcgacg acatcgaggc gattaggggg ctggaagcct atgcgggcga
     1681 tgcgggcttc cgagagaagt tcgccgccgt taagcgccag aacaaggtgc ggctggccaa
     1741 tctcgtcgcc gagcggctgg gcatcaaggt cgatccctcc gccctgttcg acatccagat
     1801 caagcgcatt cacgaatata agcgccagtt actgaacatt ctggaagccg tggcgcttta
     1861 cgaccagatc cgctcgcatc ccgaacgcga atggacgccg cgcgtgaaat tcttcggcgg
     1921 caaggcagcg cccagctacc acaacgccaa gctcatcata aagctggcca acgatgtcgc
     1981 gcgcgtcatc aacagcgacc cggccgtgca cggcctgctg aaaatcgtgt tcgtgccgaa
     2041 ctacaatgtc agcctggccg agatcatgat gccggccgcc gacctttcgg agcaaatctc
     2101 caccgccggc atggaggcgt cgggcaccgg caacatgaag tttgcgctga acggcgcttt
     2161 gaccatcggc acgctcgacg gcgccaatgt cgagatcaag gagcgcgtcg gcgacgacaa
     2221 tatcttcatc ttcggcctga cgacgcaaga agtggccgag cgccgcagca atggctacca
     2281 gccgcgcgcg gtcatcgaag gctcgccgga actgtcgcag gcgctggcgg ccatttcatc
     2341 gggcgtgttc tcgcatgacg atccgacccg ctaccgcgaa ttgatcgacg gtctctacaa
     2401 cagcgactgg tttatggtgg cggcggattt cgatgcctat gccgccaccc agcgcacggt
     2461 tgatgcggtc tggcgcgaca gccccgactg gcaggcgcgc gccatccgca acgttgcgcg
     2521 cgtcgcctgg ttctcctccg ataggacgat ccgcgaatat gcgaaagaaa tctggaacgt
     2581 atctgcctga tgtgattgca tgagaccaca ttccggtggc cgggcgcttt gggagggcgc
     2641 cgcttgatga gaaaaccgcg cgcgaccgca gcgtctgccg agccggagga acgagccgcg
     2701 gccagcgatg tcgcagccat tgtcgccggc acccacggcg atccgttcgc ggtcctcggc
     2761 gtgcagcagg cgggcggcgc attcgtggcg cgctgcttcg ttccgggcgc gcaagccgtc
     2821 accgcctgca cgctggccgg caaggcggta ggcgaactgg cgcggaccaa cgatgccggc
     2881 ttcttcgagg gcaggatttc catccgcaag cgccagccgc tgcgttatca cgccaaaaat
     2941 gccggcggcg actggtgggt ggctgatcca tattcgtttg gcccggtgct ggggccgatg
     3001 gatgattatt acatcgccga aggctcgcat ctgcgcctgt tcgacaagct cggcgcgcat
     3061 ctcatcgacc acgaaggcgc gtcgggggtg cagttcgccg tgtgggcgcc gaacgccagg
     3121 cgggtctccg tcgtcggcga cttcaacgaa tgggacggcc gccgtcatcc gatgcgcgac
     3181 cgccgcgaca ccggcatatg ggaattgttc attcccgata tcggcgccgg ccgcgcctat
     3241 aaatatgaaa tccttggggc ggatggcgtc cggctaccgc tgaaagccga cccgtttgcc
     3301 ttcaaatccg aactgcgccc ggcaaccgca tcggtgacgg ccgagccgcc
//
LOCUS       DASYCS010000385         7022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1266785,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000385 DASYCS010000000
VERSION     DASYCS010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 7022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7022
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1266785"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /locus_tag="VGM46_11975"
     CDS             <1..984
                     /locus_tag="VGM46_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013530249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4193354.1"
                     /translation="LLGPSGCGKTTLMRMLAGFEEPTQGRILLQGKDISGVPPYKRPT
                     NMMFQSYALFPHMSVERNVAFGLEQDKLPKPEIDARVEEMLTLVKLKEFAKRKPSQLS
                     GGQRQRVALARSLAKRPKVLLLDEPLGALDKKLREETQFELMDLQQNLGLTFVVVTHD
                     QEEAMTMADRIAILDKGEVMQVATPAEVYEAPGSRFVAGFVGNVNMFEGKVASRDATT
                     ARITGGSGAEILVDNVGNATAGADVAFAVRPEKIKVSSKKPKDAVNALEGEVYDIAYL
                     GDMTVYHVKLDDGQVVRASSLNAARIMEDPLTWNDRAWVSFLPDAGIVLTR"
     gene            993..1964
                     /locus_tag="VGM46_11980"
     CDS             993..1964
                     /locus_tag="VGM46_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006329359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY4193355.1"
                     /translation="MSNDMMSSTHAPLVAVEKSGLARFGSAFFSRLVIIVPYVWLLFF
                     FLVPFIIVFKISLSQTAIAIPPYTPVLGLGEGFSGFIGQLRELNFDNYTWLTQDPLYF
                     YAYVSSVVIAAISTVLTLLVGYPIAYGMARAPATIRPTLLMLVILPFWTSFLIRVYAW
                     IGILKPEGLLNQFLLATGIIHQPLIILNTYTAIFIGIVYSYLPFMVLPLYSSLEKMDY
                     SLIEAAKDLGCPPTAAFWKITFPLSLPGVIAGSLLVFIPAVGEFVIPDLLGGSQTLMI
                     GKTLWNEFFSNRDWPVSSAVAVILLLLLIVPIVLFQQAQARAQEQDR"
     gene            1966..2784
                     /locus_tag="VGM46_11985"
     CDS             1966..2784
                     /locus_tag="VGM46_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008936191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY4193356.1"
                     /translation="MNTSWSRFNITSIVLGFAFLYLPIVLLVVFSFNASKLVTVWGGF
                     STKWYVSLFHNQGLMDAAWVTIRVGLVSATVATVLGTLGALALTRYTRFKGRMLFSGM
                     IFAPLVMPDVITGLSLLLLFVAIGLDRGLLTVTLAHITFTMCFVAVVVQSRLVTMDRS
                     LEEAAMDLGAPPVKTFFQITLPVILPAIVSGWMLAFTLSLDDLVIASFASGPGATTLP
                     MKIYSQVRLGVTPEINAACTILIAIVAMGVIAASVANKRREVQRLRDEQAAQRG"
     gene            complement(2833..4692)
                     /locus_tag="VGM46_11990"
     CDS             complement(2833..4692)
                     /locus_tag="VGM46_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013530252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEY4193357.1"
                     /translation="MPSPLVRRGIWTIGIAVVVAALAFMALPYLASTRIVRDRIAWEM
                     GAWSGYRVTIEGRPHIVIWPKFRAVLSDVTLSDWARPDDPPVLQAESVEIDLSAMAAL
                     RGDVVFSTARLIRPTLRLKPAEKGLLLPAIPSGGRFARSIDTARALVAADANNPDVSK
                     LPADPFGTVEFTDGRVVALAGGKDTDVATGLNGRVNWAALNGGGQFSATGIWRGESIA
                     VDMASAKPLLLFGGGIAPASVSLKAAPATFSFEGNASLSQNAYLDGAVKFSAPSVRRA
                     LDWLQAGIAPGAAIGAVSFSSKVTAGSGRVKFDNAQIALNNNPGTGALDFSFTEARPV
                     MAGTLAFDTLDLSAFLSAFTPLALPGGQDAKDTDASFANRLDLDLRLSAAHAMAGTVQ
                     LADVAATAQVKNGLAVFDISDATAFGGNVQASVRLDRKPQGTEAEIRLLAADVNGASF
                     GAAAGMTRLVPTGTGTVSLILKGPGKAWDSILESADGSISATFGAGTLTGLNLPAFLK
                     RSQQGGFFALDDVADGTVPVDGVELKATISKGVARIDKAEANSAKSKIWLTGIVPYAG
                     RGLALSGGIIQPNQTAPADNGQAPTTPSQASFFVGGTWTTPFISPIKTGVSGQ"
     gene            4865..6823
                     /locus_tag="VGM46_11995"
     CDS             4865..6823
                     /locus_tag="VGM46_11995"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006206213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HEY4193358.1"
                     /translation="MATDTPLWTPTKEQVEAAPMTAFMRAAAEKSGLALSSYADLHRW
                     SSDDREAFWSLVWDFCGIIGDKGDVVLDAGDKMPGASFFPEAKLNFAENLLRKTGAGE
                     AIVFRGEDKVERRLSWNELHALTSRLQQLLVSLGVKAGDRVAAMMPNMPETIAAMLAT
                     VSIGAVWSSCSPDFGEQGVLDRFGQIEPVVFIAPDGYWYNGKAIEVAAKVAAVTAKMP
                     SLRKVLIVDYLGTAGDVAAGINGADALEAALAPFPVKPVTFQLLPFSHPLCILFSSGT
                     TGIPKCIVHSAGGTLIQHVKELRLHAGILEDDRLFYFTTCGWMMWNWLVSGLACGATL
                     LLYDGSPFHPDGNTLFDFADAEKMTHFGTSAKFIDSVRKAGLRPIDTHDLSTVRTISS
                     TGSPLSPEDFRFVYDGIKQDVHLASISGGTDIVSCFVLGVPIEPVWTGEIQAAGLGLA
                     VDVWNDAGQPVRQEKGELVCTKAFPAMPIGFWNDPEGKRYHAAYFERFDNVWCHGDFA
                     EWTEHGGMVIHGRSDATLNPGGVRIGTAEIYNQVEQMPEIAEAICIGQDFDNDVRVVL
                     FVRLAKGVTLDAELEKRIRARIRTGASPRHVPARIVAVTDIPRTKSGKITELAVRDAV
                     HGREIKNKEALANPEALELFRNRSELAV"
BASE COUNT         1235 a         2270 c         2164 g         1353 t
ORIGIN      
        1 ctgctcggac catccggttg cggcaagacc acgctgatgc gcatgctggc cggcttcgag
       61 gagccgacgc aaggccgcat cctgctgcag ggcaaggaca tctcgggcgt cccgccctac
      121 aagcgcccga ccaacatgat gttccagagc tatgcgctct tcccgcacat gtcggtggag
      181 cggaacgtcg ccttcggcct ggagcaggac aaactgccga aaccggagat cgatgcgcgg
      241 gtcgaggaaa tgctgacgct ggtcaagctc aaggagttcg ccaagcgcaa gccgagccag
      301 ctttccggtg gtcagcgcca gcgtgtcgcg ctcgcccgtt cgctcgccaa gcggccgaag
      361 gtgctgctgc tggatgaacc gctcggcgcg cttgacaaga agctgcgcga ggaaacccag
      421 ttcgaactga tggatttgca gcagaacctc ggcctcacct tcgtcgtcgt cacgcacgac
      481 caggaagaag ccatgaccat ggccgaccgc atcgccattc tcgacaaggg cgaggtcatg
      541 caggtggcga cgcccgccga ggtctacgag gcgcccggct ctcgcttcgt cgccggcttt
      601 gtcggcaatg tgaacatgtt cgagggcaag gtagcctcgc gcgatgccac cacggcccgc
      661 atcaccggcg gcagcggcgc ggaaatcctc gtcgataatg tcggcaacgc caccgccggc
      721 gccgatgtcg ccttcgccgt tcgccccgaa aagatcaagg tgtcgtcaaa gaagccgaag
      781 gatgccgtca acgcgctgga gggcgaggtc tatgacattg cctatctggg cgacatgacg
      841 gtctatcacg tcaagctcga tgacggccag gtggtgcggg cgagcagcct caacgccgcg
      901 cgcatcatgg aagacccgct gacatggaat gaccgcgcct gggtatcctt cctgccggat
      961 gccggcatcg tgctgacgcg gtaggggagg ctatgtcgaa cgacatgatg tcatccaccc
     1021 acgcgccgct ggtcgccgtg gaaaaatccg gccttgcccg gttcggctcg gccttcttca
     1081 gccggctggt catcatcgtt ccctatgtct ggcttttgtt cttcttcctc gtccccttca
     1141 tcatcgtctt caagatatcg ctgtcgcaga cggccatcgc cattccgccc tatacgccgg
     1201 tgctcggcct gggcgagggc ttcagcggct tcatcggcca attgagggaa ctcaatttcg
     1261 acaactacac ctggctgacg caggacccgc tctatttcta cgcctacgtc tccagcgtcg
     1321 ttatcgccgc aatctccacg gtgctgaccc tgctggtcgg ctaccccatc gcctacggca
     1381 tggcgcgcgc gccggccacc atccgcccga cgcttttgat gctggtgatc ctgccgttct
     1441 ggacttcgtt cctgatccgc gtctacgcct ggatcggcat cctgaagccg gaagggctgc
     1501 tcaaccagtt cctgctcgcc accggcatca tccatcaacc gctgatcatc ctcaacacct
     1561 acaccgccat cttcatcggc atcgtctatt cctacctgcc gttcatggtg ctgccgcttt
     1621 attcctcgct cgaaaagatg gactattcgc tgatcgaagc ggcaaaggac cttggctgcc
     1681 cgccgaccgc ggcgttctgg aagatcacct ttccgctgtc gctgcccggc gtgattgcag
     1741 gcagcctgct ggtgttcatt cccgccgtcg gcgaattcgt gatccccgac ctgctcggcg
     1801 gctcgcagac gctgatgatc ggcaagacgc tgtggaacga gttcttctcc aaccgcgact
     1861 ggccggtgtc gtcggcggtc gccgtcatct tgctgctgct gctgatcgtc cccatcgtac
     1921 ttttccagca ggcgcaggcg cgcgcccagg aacaggaccg ctgacatgaa cacgagctgg
     1981 agccgcttca acatcacctc gatcgtgctt ggctttgcct tcctctattt gccgatcgtg
     2041 ctgctcgtgg tgttctcttt caacgcctcg aaactggtca ccgtgtgggg cggcttttcg
     2101 accaaatggt atgtgtcgct gttccacaac cagggcctga tggacgccgc ctgggtcacc
     2161 atccgcgtcg gcctggtgtc ggcgacggtg gccacggttc tgggtacgtt gggcgcgctg
     2221 gcgttgacgc gctacacgcg cttcaaggga cgcatgctgt tttcgggcat gatattcgcg
     2281 ccgctggtca tgcccgatgt catcaccggc ctgtcgctgt tgctgctgtt cgtcgccatc
     2341 ggcctcgatc gtgggctgtt gacggtgacg ctggcgcaca tcaccttcac catgtgcttc
     2401 gtggccgtgg tggtgcagtc gcggctggtc acgatggacc ggtcgctgga agaggccgcc
     2461 atggatcttg gcgcgccgcc ggtcaaaacc tttttccaga tcacgctgcc ggtcatcctg
     2521 ccggccatcg tctccggctg gatgctggct ttcaccttgt cgctggacga tctggttatc
     2581 gcctccttcg cttccggtcc gggcgccacc acgctgccga tgaagattta cagtcaggtg
     2641 cgcctgggcg tcacgccgga aattaacgct gcctgcacca tcctgatcgc catcgtcgcc
     2701 atgggcgtga tcgcggcttc cgtcgccaac aagcgccgcg aggttcagcg cctgcgcgat
     2761 gaacaggcgg cgcaaagagg ctgagcgcaa gatcgatcga cgatcaacgg ccgggcggtt
     2821 ggcctgccgg tgttattgcc cggaaacgcc cgttttgatc ggtgagatga agggcgtggt
     2881 ccaggttcca ccgacgaaga acgacgcctg gcttggcgtt gtcggcgcct ggccattatc
     2941 ggccggcgcc gtctggttcg gctggatgat gccgcccgac agcgccaggc cccggccggc
     3001 ataaggcacg atgccggtca gccagatctt gcttttggcg gagttggcct cggccttgtc
     3061 gatccgcgcc acgcccttcg aaatggtcgc cttgagttcg acgccatcga ccggaacggt
     3121 gccgtcggca acatcgtcga gcgcgaagaa gccgccctgc tggctgcgtt tgaggaaggc
     3181 gggcaggttg aggccggtca gggttcccgc gccgaaggtc gccgaaattg agccgtcggc
     3241 gctttcaagg attgaatccc acgccttgcc cggccctttc aggatcaagg aaaccgttcc
     3301 cgtgccggtc ggcaccagcc gcgtcatccc ggccgctgcg ccgaaactcg cgccattgac
     3361 atcggcggca agcaggcgga tttcggcttc cgtgccctgc ggcttgcggt ccaggcgcac
     3421 gctggcctgc acattgccgc caaaggcggt ggcatctgaa atatcgaaga cggcaagtcc
     3481 gttctttacc tgcgcggtgg cggcgacatc ggccagctga acggtgcctg ccattgcatg
     3541 ggcggccgac agtcgaaggt cgaggtccag ccggttggcg aagctcgcat cagtgtcctt
     3601 ggcgtcttgc ccgccgggca gggcaagcgg cgtgaaagcc gaaaggaagg cgctcaaatc
     3661 gagcgtatcg aaggcgagcg tcccggccat gacgggccgc gcctcggtaa acgaaaaatc
     3721 gagcgcgccg gtgccgggat tattgttcag cgctatctgg gcattgtcga atttcacccg
     3781 tccgcttccc gccgtgacct tgctggaaaa cgaaaccgcg ccgattgcgg cccccggcgc
     3841 gatgccggcc tgcaaccagt ccagcgcccg gcgcaccgag ggggcagaaa atttcacggc
     3901 gccgtcgaga taggcgttct gggaaaggct ggcattgccc tcgaacgaga atgtcgccgg
     3961 cgcggccttc agggaaacgc ttgccggcgc gatcccgccg ccgaacaaca gcagcggctt
     4021 ggccgacgcc atgtcgaccg cgatgctttc gccgcgccag atgccggtcg ccgaaaactg
     4081 gccgccgccg ttcagcgccg cccagttcac cctgccgttg aggccggtgg ccacgtccgt
     4141 gtccttgccg ccggccagcg cgacgacccg gccgtcggtg aattccaccg tgccgaacgg
     4201 atcggccggc agcttgctga cgtcggggtt gtttgcatcg gcagcgacga gcgcgcgcgc
     4261 ggtgtctatc gagcgggcga aacgcccgcc ggatggaatg gccggcagca acaggccttt
     4321 ttcagccggc ttgagccgca gcgtcggccg gatgaggcgg gcggtcgaaa acaccacgtc
     4381 gccccgaagt gcggccattg ccgaaagatc gatctcgacg ctctcggcct gcaacaccgg
     4441 cggatcgtcg ggccgcgccc agtccgatag cgtcacgtcg ctgaggacag cccgaaattt
     4501 cggccagatg acgatgtgcg gccggccttc gatggttacc cggtaaccgc tccaggcgcc
     4561 catttcccag gcgatgcgat cgcgcacgat gcgcgtcgag gccaggtagg gcagggccat
     4621 gaacgccagc gcggcgacga ccacggcgat gccgatcgtc catatgcctc gccggaccag
     4681 aggtgatggc atgtagcccc gtatcttcac atgcttcgca atcgccgcaa gatgtaaccg
     4741 acgcctgacg cattacaagt cttaatgggc aggtgagggc atttgcacac aatgcggcgc
     4801 gaagaaaatc ttgatgacgc gggccaatat gtttaaccag caggccaaag ggagagcaaa
     4861 acgcatggcc actgatacac cgctatggac tccgacgaag gagcaggtcg aggccgctcc
     4921 gatgaccgct ttcatgcggg cggctgcgga aaaatcgggc ctggcgcttt cctcgtatgc
     4981 cgatctccac cgctggtcga gcgatgaccg cgaggcgttc tggagcctgg tctgggattt
     5041 ttgcggcata atcggcgaca agggcgatgt ggttctggac gccggcgata agatgcccgg
     5101 tgcttccttc tttcccgaag cgaagctgaa tttcgctgaa aacctcctgc gcaagaccgg
     5161 cgccggcgaa gcgatcgtct ttcgcggcga agacaaggtc gagcggcggc tgtcgtggaa
     5221 cgagctgcat gcgctgacat ctcgcctgca acaattgctg gtttcgctgg gcgtgaaggc
     5281 cggcgaccgc gtcgccgcca tgatgcccaa catgcccgaa acgattgccg ccatgctggc
     5341 caccgtgtcg atcggcgcgg tgtggtcgtc ctgttcgccc gacttcggcg agcagggcgt
     5401 gctcgaccgc ttcggccaga tcgaaccggt ggtgttcatc gcgcccgatg gctactggta
     5461 taacggcaag gccatcgaag ttgccgccaa ggttgcggcg gtgaccgcca agatgccctc
     5521 gctgcgcaag gtgctgatcg tcgattatct cggcaccgcc ggcgacgtag cggcgggcat
     5581 caacggcgcc gatgcgctgg aggccgcgct tgcgccgttt ccggtcaagc cggttacgtt
     5641 ccagcttctg ccgttttcgc acccgctctg cattctgttt tcgtcgggca ccaccggcat
     5701 tcccaaatgc atcgtccact ccgccggcgg cacgctgatc cagcacgtca aggaattgcg
     5761 gctgcacgcc ggcatcctcg aagacgaccg cctgttttac ttcaccacct gcggctggat
     5821 gatgtggaac tggctggtgt cgggcctggc ctgcggcgcg acgctgctgc tctatgacgg
     5881 ttcgcctttc caccccgatg gcaacacgct gttcgacttc gccgatgcgg aaaagatgac
     5941 gcatttcggc acctcggcca aattcatcga ttcggtgcgc aaggccggcc tgcgccccat
     6001 cgatacgcat gacctctcca ccgtgcgcac catctcttcc accggatcgc cactgtcgcc
     6061 ggaagatttc cgcttcgtct atgacggcat caagcaggat gtgcatctgg cctccatctc
     6121 gggcggcacc gatatcgtct cctgcttcgt gcttggcgtg ccgatcgagc cggtgtggac
     6181 cggcgagatc caggcagccg gccttggcct ggccgtcgat gtctggaacg atgcgggcca
     6241 gccggtgcgg caggaaaagg gcgaactggt ctgcacaaaa gcctttccgg caatgccgat
     6301 cggcttctgg aacgatcccg aaggaaaacg ctaccacgcc gcctatttcg agcgcttcga
     6361 caatgtctgg tgccacggcg atttcgccga gtggaccgaa catggcggca tggtcatcca
     6421 tggccggtct gacgccacgc tgaaccccgg cggcgtgcgc atcggcacgg cggaaattta
     6481 caaccaggtc gagcagatgc cagagatcgc cgaggcgatc tgcatcggcc aggatttcga
     6541 caatgacgtg cgcgtcgtcc tgttcgtgcg gctggcgaag ggcgtcaccc tcgacgccga
     6601 gttggaaaag cgcatccgcg ccaggatacg caccggcgcc agcccgcgcc acgtaccggc
     6661 ccggatcgtc gccgtcaccg acataccgcg caccaaatcc ggcaaaatca ccgaactcgc
     6721 cgtgcgcgac gctgtccatg gccgcgaaat caagaacaag gaagcgctgg ccaatcccga
     6781 agcgcttgag ctgtttcgaa accgctccga gcttgcggtt tgatgcggct aggcaacatg
     6841 tttaacgaaa ggtttcgcgc aaatttacct aaccttcaga cagggtacgg attcgtaaag
     6901 tttccgcgcg cccggtaagg atttgttaag tcccgccgcc gatagtcgca accaacttga
     6961 gggtatcccc gttctcccgt gaggacattc gtactcaagc ccgttgtgac caatctcatc
     7021 at
//
LOCUS       DASYCS010000386         8830 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1417648,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000386 DASYCS010000000
VERSION     DASYCS010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 8830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8830
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1417648"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..141
                     /gene="rlmJ"
                     /locus_tag="VGM46_12000"
     CDS             <1..141
                     /gene="rlmJ"
                     /locus_tag="VGM46_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HEY4193359.1"
                     /translation="GSGLVVVNPPFTLEGELRTILPALHRLLAVEQPSRWNLEWLAGE
                     AA"
     gene            226..678
                     /locus_tag="VGM46_12005"
     CDS             226..678
                     /locus_tag="VGM46_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010915202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193360.1"
                     /translation="MTRFTASILAALVGICAPLAATPAAFAADPVGYVDNGDAGDAGI
                     CSQAWVLSKISSRFHHQVQHVPNLPNVDITDFRRIHQHRYLPASENSPIGRSYCGATV
                     ALSDGQSREIWYLIEEGQGFASIGDNVEFCVSGFDRWNVYNGRCRVLR"
     gene            689..1441
                     /locus_tag="VGM46_12010"
     CDS             689..1441
                     /locus_tag="VGM46_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019170530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease"
                     /protein_id="HEY4193361.1"
                     /translation="MGRPRWKSLPVLAVGLLCLTACAPSGSEKHEADASPKAEKAGAP
                     ARANGDSVRKAALPQGSGFDFYVLSLSWSPSYCEAEGANANRQQCSTSRPYAFVVHGL
                     WPQFERGYPQKCGTDEMKVSGQTVRKLYDLMPSAGLIRHEWQTHGSCTGLSQFDYFDV
                     LRAARRKVAIPSDFRRLDDYRSLSPGEAEAQFLQANPDLPPDGISITCDRRFLREVRI
                     CMTKDLAFRPCDEIERRSCRLRKIVMPPVRGG"
     gene            1515..2111
                     /locus_tag="VGM46_12015"
     CDS             1515..2111
                     /locus_tag="VGM46_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEY4193362.1"
                     /translation="MPKLLYAPASPYSAKVRMAAVYAGIDIEGVNTATDERPAQLIGA
                     NPLGKIPTLLLDDGRSIYDSRAITQYLNRLSKNALFPRNPDKRTDAEVLEAMADGICD
                     SALAMVYERRTRPEEMVYQPWLDRQWLKISAALDMLNANPPKLPKKITVGQIALRACL
                     GYLALRFAGKWEKGHGRLTRWAARFDEKFPELKAYIPS"
     gene            complement(2242..2898)
                     /locus_tag="VGM46_12020"
     CDS             complement(2242..2898)
                     /locus_tag="VGM46_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019857308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein"
                     /protein_id="HEY4193363.1"
                     /translation="MKANLKHVRPLAAALGLLAFAGVPAFAADVVSEEPPAPAPVAEL
                     PLASWAGPYAGINLGYGFSGRTDDGGNRFKTDGFIGSGFVGYNWQMDNFVLGAEGELG
                     YNGVKGGNDLSESKSGFEGSLRARLGYAVTPEILLYGTAGGAGQSLKVSDATGSDKNS
                     MFGWTAGVGTDIKLTDNIFGRVEYRYTDYGSKDFTTPLGTRDVDSHDNRVTFGVGMKF
                     "
     gene            complement(3247..3600)
                     /locus_tag="VGM46_12025"
     CDS             complement(3247..3600)
                     /locus_tag="VGM46_12025"
                     /inference="COORDINATES: protein motif:HMM:NF014233.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEY4193364.1"
                     /translation="MQRTVIVRDLELMLSIGVHAHEKLAPQRLVVSVEATLSGEGDAA
                     DNIAATLDYDRICDFIRAIGNEGHVELQETVARRVLAFVLSLPGVASAVVETRKPDVF
                     DDCAFVGARLHGCNE"
     gene            complement(3628..4392)
                     /locus_tag="VGM46_12030"
     CDS             complement(3628..4392)
                     /locus_tag="VGM46_12030"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY4193365.1"
                     /translation="MAATEQPRPVCLITGGARRLGAAIATHLAPRFDLAIHYRSSAQE
                     AEGLAARLREKGGRVELFQADFADHDDTARLVPEAVERMGRIDMMVSSASQFDFDTPS
                     EFSPAVMQRLLSVNLVAPMILACELALAGSPEATLVHMLDSKVFSLNPDFFSYSLSKI
                     ALKGSIEMQAMHFRGKLRVCGIAPSVTLVSGDQSPENFEKSWRHTLTGSGPTPQDIGG
                     AVEFIWDTKSLNGSTITLDGGQHLMGLQRDVAFVVE"
     gene            4755..6809
                     /gene="uvrC"
                     /locus_tag="VGM46_12035"
     CDS             4755..6809
                     /gene="uvrC"
                     /locus_tag="VGM46_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010915212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEY4193366.1"
                     /translation="MSSDESKVARGTTANGDLPDVDLDDDGDDAAVAEPTGADVAFTA
                     IDWTPHAGDADGMVGAEVIQTLVKRLPNAPGVYRMMNAAGDVLYVGKARSLKKRVTNY
                     AQGRFHTNRIGRMVRETATMEFVVTRTEIEALLLEANLIKRLRPRFNVLMRDDKSFPY
                     ILLTGNHPSPGIYKHRGARSRDGDYFGPFASAGAVGRTINSLQRAFLLRSCTDSFYEN
                     RTRPCLLFQIKRCAGPCTGEISHGDYAELVGEAKDFLSGRSQKVKTEISSAMQEASDA
                     LDFERAAIYRDRLAALSHVQSHQGINTQTVDEADVFAIHQEGGQVGIQVFFFRTGQNW
                     GNRAYFPKADPALEAAEVLGSFIAQFYDDKPTPRTILLSHEVAEQGLLGEALSARAGR
                     KVTISVPQRGEKKELTDNALQNAREALGRRLAETSTQTRLLAGFAETFGLEKPPVRIE
                     VYDNSHIMGTNAVGTMIVAGPEGFVKNQYRKFNIRSTEITPGDDFGMMREVMERRFSR
                     LLKEHGEAAPGEAAGNDEENGDTGFPAWPDVILIDGGQGQMTAVRQILADLGIEDRVI
                     AIGVAKGQDREAGRERFFVRGKTSFSLPVRDPVLYFVQRMRDEAHRFAIGTHRARRKK
                     ELVKNPLDEISGIGPGRKRALLMHFGTAKAVSRAAIEDLTTVDGISEQVARLVYNHFH
                     ES"
     gene            6912..7508
                     /gene="pgsA"
                     /locus_tag="VGM46_12040"
     CDS             6912..7508
                     /gene="pgsA"
                     /locus_tag="VGM46_12040"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006201585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEY4193367.1"
                     /translation="MAKRAFNLPNMLTYARIVAVPLVVLCFFLEGHLKSSDFARWSAL
                     IIFLLASITDYFDGYLARAWQQTSNIGRMLDPIADKLLVSTLLLLLAADTDRQAGIAG
                     WSLWAAIIILCREILVSGLREYLAALKVSVPVTRLAKWKTAIQMVAIAFLLAGPAGDK
                     IFPFTTQTGLVLLWVAAIVTLYTGYDYFRAGLKHIVDE"
     gene            7517..7768
                     /gene="moaD"
                     /locus_tag="VGM46_12045"
     CDS             7517..7768
                     /gene="moaD"
                     /locus_tag="VGM46_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018427135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HEY4193368.1"
                     /translation="MKLIYFAWVRERIGKPEEDVDLPANVETVQDLLRWLKSRGEEYE
                     NALEYPDVIRVAINHEHVDHREKIAGAREIALFPPMTGG"
     gene            7773..8243
                     /locus_tag="VGM46_12050"
     CDS             7773..8243
                     /locus_tag="VGM46_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006331815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEY4193369.1"
                     /translation="MPEKPLPTVRIQHQDFDVAAEIGKLTEGRVDIGAVVTFLGLCRG
                     EDGALSALELEHYPGMAEAEIRRIADEAVLRWPLQGLTAIHRFGKIRPGENIVLVLAA
                     SSHRQAAFEAANFLMDFLKSRAPFWKKEHHADGTAGGWVDAKEADERAALRWKR"
     gene            8338..8754
                     /locus_tag="VGM46_12055"
     CDS             8338..8754
                     /locus_tag="VGM46_12055"
                     /inference="COORDINATES: protein motif:HMM:NF021158.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HEY4193370.1"
                     /translation="MLDRIAELFVFGFTPMVIGMLAVPAVSIAGEQVMRGEVLYRERI
                     ALPPNAVLWVQLADVSKADAPAAILSEQKIDPAGQVPIKFEIRFDPSVIRATMSYALQ
                     ARITVDGKLWFVTDTHHPVDPLSDKPQTVLVKRVAR"
BASE COUNT         1690 a         2616 c         2772 g         1752 t
ORIGIN      
        1 ggcagcgggc tggtggtggt caacccgcca ttcacgctgg agggtgaatt gcggacgatc
       61 ctgcccgcgc ttcaccggct gctggccgtg gaacagccgt cgcgctggaa cctcgaatgg
      121 ttggccggcg aggcggcctg atatcgccgc aagtcctgcc ttgtggtggc ttgaccggaa
      181 caccgctatt ccgcagagtg gaggctgtcg ttgaaagaga ccaacatgac tcgttttacc
      241 gcttccattc ttgccgcgct ggtgggcatc tgcgcgccgc tggccgccac accggcagcg
      301 tttgccgccg atccggtggg ttatgtcgac aatggcgatg cgggcgacgc cggcatttgc
      361 agccaggcct gggtgctcag caagataagc tcgcggttcc accatcaggt ccagcatgtg
      421 ccgaacctgc ccaacgtcga cattaccgat ttcaggcgca ttcatcagca ccgctacctg
      481 ccggccagcg aaaattcgcc gatcggccga agctattgcg gcgccacggt tgcgctttcg
      541 gacgggcaaa gccgtgaaat ctggtatctg atcgaggaag gccagggttt cgcgtcgatt
      601 ggcgacaatg tcgaattctg cgtctcgggt ttcgaccgct ggaacgtcta taacggccgc
      661 tgccgcgttc ttcgctgatt cgcagccgat gggccggccg cgctggaaat cgctgccggt
      721 attggctgtc ggtctgcttt gcctgacagc ctgcgcgcct tcgggcagcg aaaagcatga
      781 ggcggatgcc tcgccgaagg cggaaaaggc cggcgcgcct gcccgcgcga acggtgattc
      841 ggtgcgcaaa gccgcgttgc cgcagggcag cggctttgat ttctacgtgc tgtcgctatc
      901 gtggtcgcca agctattgcg aagcggaagg cgcaaacgcc aaccgccagc agtgcagcac
      961 ttcgcgcccc tatgctttcg tcgtccatgg gctgtggccg caattcgagc gtggctatcc
     1021 gcaaaaatgc ggtacggacg agatgaaggt ttccggtcag accgtgcgca agctctatga
     1081 tctgatgccc tcggcggggc tgatccgcca cgagtggcag acgcatggca gttgcacggg
     1141 gctgagccag ttcgattatt tcgatgtgct ccgcgcggcg cgccggaagg tagccattcc
     1201 ttccgacttt cgccgcctcg acgactatcg ctcgctttcg ccgggcgagg ccgaagcgca
     1261 atttctccaa gccaatcccg atttgccgcc cgatggcatc tccatcacct gcgaccggcg
     1321 ttttttacgc gaggttcgta tctgcatgac caaggatctg gcttttcggc cgtgcgacga
     1381 gatcgaacgg cgttcgtgcc ggcttcgaaa aatagtcatg ccgccggttc gtggtggata
     1441 attttgccgt atctggcaat tgcccgctgt gcctttccca agcatagctt gtttcgaaac
     1501 aacaggaata tcccatgcca aaactgcttt atgctcccgc ttcgccctat agcgccaagg
     1561 tccggatggc ggccgtctat gccggcatcg acatcgaggg cgtgaacacg gctaccgacg
     1621 aacggccggc gcaactaatc ggggccaacc cgctgggaaa aatcccgacc ctgttgctgg
     1681 atgacggccg cagcatctat gacagccgcg ccattaccca atacctcaac cgcctgtcga
     1741 aaaatgcgct gtttccgcgc aatcccgaca agcgcaccga tgcagaagtg ctggaggcga
     1801 tggctgacgg catttgcgac agcgcgctgg cgatggtcta tgagcggcgt acgcggccgg
     1861 aagagatggt ctatcagcct tggctggacc ggcaatggct gaagatttcg gcggcgctcg
     1921 acatgctgaa cgccaaccca cccaagctgc cgaagaaaat caccgttggc cagatcgcct
     1981 tgcgcgcctg cctcggctat ctggcgctgc gctttgccgg aaaatgggaa aagggccatg
     2041 gccgcctgac ccgctgggca gctcggttcg atgaaaagtt tcccgagttg aaggcttata
     2101 taccctcgta accgttcagc gcgccgcgtt cacggctgtc gggaaacgaa aagccgggca
     2161 agtgtcgaaa aggcggctca agaatcgaaa aggccgggct ttgaagcccg gcctttccgc
     2221 attcaagagg aggctggtcg atcagaactt catgccgacg ccgaaggtga cgcggttgtc
     2281 gtgcgaatcg acgtcgcggg taccaagcgg ggtggtgaag tccttgctgc cgtagtcggt
     2341 gtagcggtat tcgacgcgac cgaagatgtt gtcggtcagc ttgatgtcgg taccgacgcc
     2401 ggccgtccag ccgaacatcg agttcttgtc gctgccggtg gcatccgaca cttttaggct
     2461 ctggccagcg ccgccggcgg tgccgtacag cagaatttca ggcgtcacgg catagccgag
     2521 gcgagcacgc agcgagcctt cgaagccgga cttggattcc gacaggtcgt tgccgccctt
     2581 gacgccgttg tagccgagtt cgccttcggc gccgagcacg aagttgtcca tctgccagtt
     2641 gtatccgacg aagccgctgc cgatgaagcc atcggtcttg aagcggttgc cgccatcatc
     2701 ggtacggccg ctgaagccgt agccgaggtt gataccggca taaggaccgg cccaggaagc
     2761 taagggcaac tcggcaacgg gagccggtgc gggtggctct tccgagacga cgtcggcggc
     2821 aaaagcagga acgccggcaa aggcgagaag cccgagcgcc gcagcaagcg ggcgcacatg
     2881 tttgaggttg gctttcattt atctaactcc ttgagcctcg gtacacaggc tgattatggt
     2941 gagcataccc actcgttcgg cccgctggcc tgggagagga aacgggctgg ccgctcatcg
     3001 gttccaaatg tcgtgtcgaa atacggctga agcgaacctg aaaacgggtc attccctggc
     3061 gcggatatgg cagaaatcgg gcgttgccta ttggcaacag attttcgcgc aaagccttgt
     3121 gttctgcatg gttgcgggcg tttcgaccca tcttggaagg gccgattgca gcccgctatc
     3181 ggccgcatgc cacagtcttg cccgattgat tgaggcttcg ttaccgctca ttggcgaagt
     3241 taaggattat tcgttgcagc cgtggagccg ggcgccgaca aaggcgcaat cgtcgaaaac
     3301 atcgggcttt ctggtctcga cgacagcaga agccacgcct ggaagcgaca gcacgaaggc
     3361 gagcacgcgc cgggcgacgg tttcctgcaa ttccacatgg ccctcattgc ctatggcgcg
     3421 aatgaaatcg cagatgcggt cgtaatccag tgtggcggcg atattgtcgg ccgcgtcgcc
     3481 ttcgcccgac agggtggcct cgacgctgac cacaagccgc tgcggggcca gtttttcgtg
     3541 cgcgtgaacg ccgatactga gcatcagttc gagatcgcgc acgattaccg ttcgttgcat
     3601 gatttgcatg tcattccacc aggaggttca ttcgaccacg aaggccacgt cgcgttgcag
     3661 ccccatcaga tgctggccgc catcgagcgt gatggtcgag ccattgagcg atttggtatc
     3721 ccatatgaat tcgaccgcgc cgccaatatc ctgcggggta gggccgctgc cggtcagggt
     3781 gtggcgccag ctcttctcga aattctccgg cgactggtcg ccgctgacga gggtgacgct
     3841 gggcgcaatc ccgcagacgc gaagcttgcc gcgaaaatgc atggcctgca tctcgatgga
     3901 gccttttagg gcgattttcg aaaggctgta gctgaaaaaa tcggggttga gcgaaaatac
     3961 cttgctgtcg agcatatgca ccagcgttgc ctccggcgaa cccgcaagcg ccagttcaca
     4021 cgcaagaatc atcggcgcga ccagattgac cgacagcagg cgctgcatga cggctggcga
     4081 gaattccgaa ggcgtatcga aatcgaattg cgaggcgctg gacaccatca tgtcgatgcg
     4141 gcccatccgc tcgaccgctt cgggcacgag acgggcggtg tcgtcgtggt cggcaaagtc
     4201 ggcctggaac agttcgaccc tgcctccctt ttcccgcaac cgcgcagcca ggccttccgc
     4261 ctcctgcgcc gatgaacgat agtggatcgc aagatcgaag cgcggcgcga gatgggtggc
     4321 gattgccgcg cccagcctgc gtgcgccgcc ggtgatcaga cataccggcc ttggctgttc
     4381 agtcgctgcc aaaattgttt ttccttctgc ggccactgta tcgatctttc caatcgatct
     4441 tccaattgat cctcgcgggc gtcgaacttg tcagcattgt ccagcttctg cggcgatccg
     4501 gcttttggcg gtggcgatgg gcatataatg attgtcgctt gagtcgtttg aacggcgaaa
     4561 aggcatcagg aaatccctat ataggatcag cgctccatgc aggagcaaat ccatatccgg
     4621 atttctagcc aacgaagtga aatggagcca gaggtgctcg ccacccgact gccgcgattt
     4681 tgcgtgaacc gcaaagacct ttgccgccgc cacggcggca tcgcccctat gcgccttgcg
     4741 acaggcgcgc ggcgatgagt tcggacgaaa gcaaggtggc gcgtggaacg accgcaaacg
     4801 gcgatctgcc cgatgtcgat ctggacgacg atggcgacga tgcggccgtt gccgagccta
     4861 caggcgcgga tgtagccttt accgcgatag actggacgcc gcatgccggc gatgccgacg
     4921 gcatggtcgg agccgaggtc atccagacgc tggtcaagcg gttgcccaac gcgcccggcg
     4981 tctaccgcat gatgaatgcg gcgggcgacg tgctttatgt cggcaaggcg cgcagcctca
     5041 agaagcgcgt gacgaattat gcgcaagggc ggttccacac caaccgcatc ggccgcatgg
     5101 tgcgcgagac ggcgacgatg gaattcgtcg tcacccgcac cgaaattgaa gcgctgctgc
     5161 tcgaagccaa ccttatcaag cgcttgcggc cgcgtttcaa tgtgctcatg cgcgacgaca
     5221 agtcgttccc gtatatactg cttaccggca accatccgtc gcccggcatc tacaagcacc
     5281 gtggcgcgcg ctcgcgcgat ggcgattatt tcgggccgtt tgcctcggca ggcgcggttg
     5341 gccgcaccat caattcgctt cagcgcgcgt tcctgctgcg cagttgcacg gattcgttct
     5401 acgagaatcg cacgcggccc tgcctgcttt tccagatcaa gcgctgcgcg ggtccctgca
     5461 ccggcgagat ttcgcacggc gattatgccg aactggtcgg cgaggcgaag gactttttgt
     5521 ccgggcgcag ccagaaggtg aagaccgaaa tctcgtcggc catgcaagag gcttcggatg
     5581 cgctggattt cgagcgcgcc gcgatctacc gcgaccgcct ggcggcgctt tcgcatgtgc
     5641 agagccatca gggcatcaac acgcagacgg tggacgaggc cgatgtgttc gccatccatc
     5701 aggaaggagg gcaggtgggc atccaggtgt tcttcttccg caccggccag aattggggca
     5761 atcgcgccta tttccccaag gctgaccccg ctttggaggc ggcggaggtg ctgggttcat
     5821 tcatcgcgca gttctatgac gacaagccga cgccacgaac catcctgctt tcgcacgagg
     5881 ttgccgagca gggcctgctg ggagaagcgc tgtcggcccg tgccggccgc aaggtgacga
     5941 tttccgttcc gcagcgtggc gaaaagaagg aactgaccga caacgcgctg caaaatgcgc
     6001 gcgaggcgct tggccgccgg ctggccgaga cttcgacgca aacgcgcctg ctggccggtt
     6061 ttgccgagac gttcgggctg gaaaagccgc cggtgcgcat cgaagtgtac gacaactccc
     6121 acatcatggg caccaatgcg gtcggtacga tgatcgtcgc cggccccgaa ggctttgtga
     6181 agaaccagta ccgcaagttc aacatccgct cgaccgagat aacgccgggc gacgatttcg
     6241 gcatgatgcg cgaggtgatg gagaggcggt tttcgcggct gctgaaagaa catggcgagg
     6301 cagcgcccgg tgaagcggcc ggaaatgacg aggaaaacgg cgacaccggc tttcccgcct
     6361 ggcccgacgt gatcctgatc gacggcggcc agggccagat gacggcggtg cggcaaattc
     6421 ttgccgatct cggcatcgag gatcgcgtca ttgccatcgg cgtcgccaag gggcaggacc
     6481 gcgaggcggg gcgcgagcgc ttcttcgtgc gcggcaagac ttcgttttcg ctgcccgtgc
     6541 gcgatccggt gctttatttc gtccagcgca tgcgcgacga ggcgcaccgg ttcgccatcg
     6601 gcacccaccg cgcacgccgc aagaaggaac tggtgaagaa cccgctcgac gaaatcagcg
     6661 gcatcgggcc tggccgcaag cgcgcgctgc tgatgcattt cggtacggcc aaggcggtca
     6721 gccgcgcggc aatcgaggat ttgacgacgg tggacgggat ttccgagcag gtggccaggc
     6781 tggtctacaa tcatttccac gaaagttgac ccatttgccg gtgttttggg ctggccaatc
     6841 gattttcgcc tgttgacccc tcatattacc ttctgatttg agtactgaga cagaaaagag
     6901 ttccccaaga catggccaag cgcgcgttca atctgcccaa catgcttacc tacgcccgca
     6961 tcgtcgcggt gccgctggtg gtcttgtgct tcttcctcga agggcatttg aaatcgagcg
     7021 actttgcccg ctggtcggcg ctgatcattt tcctgctggc cagcatcacc gactatttcg
     7081 acggctatct ggcgcgcgcc tggcagcaga catcgaatat cggcaggatg ctcgatccga
     7141 ttgccgacaa gctgcttgtc tcgaccctgc ttttgctgct tgccgccgat accgaccggc
     7201 aggcgggcat cgccggctgg tcgttgtggg cggccatcat catcttgtgc cgcgaaatcc
     7261 tggtgtcggg gctgcgcgaa tatctggcag cgctcaaggt tagcgtgccc gtgacgagac
     7321 tggccaaatg gaaaaccgcg atccagatgg tggcgatcgc ttttctgctc gcgggtccgg
     7381 ccggcgacaa gatattcccg ttcacgacgc agaccggcct tgtgctgttg tgggtggcgg
     7441 ctatcgttac gctctatacg ggctacgatt atttccgtgc cggcctcaag catatcgtcg
     7501 acgagtaggc agggcgatga agctcattta ttttgcctgg gtgcgcgagc ggatcggcaa
     7561 gccggaagaa gatgtggacc tgccggcgaa tgtcgagacg gttcaagatc tgctgcgctg
     7621 gctgaaatcg cgcggcgagg aatatgaaaa cgcgctggaa tatccggacg tgatccgggt
     7681 tgcgatcaac cacgaacatg tcgaccaccg cgagaaaatt gcgggcgcgc gcgagatcgc
     7741 gctgtttccg ccgatgaccg ggggctgacc ccatgccgga aaaaccgctg ccgacggtgc
     7801 gcatccagca ccaggatttc gatgtggcgg ccgagatcgg caagctgacc gagggccgtg
     7861 tcgatatcgg cgcggtggtt accttcctgg gtctttgccg tggcgaggat ggcgcgcttt
     7921 cggcgctgga actcgaacat tatccgggca tggcggaggc ggaaatccgc cgcatcgccg
     7981 atgaagctgt gctgcgctgg ccgttgcagg gccttaccgc cattcaccgc tttggcaaga
     8041 tcaggcccgg cgagaatatc gtgctggtgc tggcggcgtc gtcgcaccgg caggccgcct
     8101 tcgaggcggc gaattttctc atggatttcc tgaaatcgcg cgcgcctttc tggaagaaag
     8161 agcatcacgc cgacggcacc gccggcgggt gggtcgatgc caaggaagcc gacgagcggg
     8221 ccgccttgcg ctggaagcgt taatccgcgt ttaccgcaaa tccccatctt gccgcgccgc
     8281 agtcacggat tgtccgtaat tgcgggcatt tatctgcttc tgaaagggag cagggccatg
     8341 ttggaccgga tagccgaact ttttgttttc ggatttacgc cgatggtcat cggcatgctg
     8401 gccgttccgg ctgtctccat tgccggcgaa caggtcatgc gcggcgaggt gctttaccgc
     8461 gagaggatcg cgttgccgcc caatgccgtg ctttgggtgc agcttgccga tgtttcgaag
     8521 gcggacgcac ccgcggccat cctcagcgag cagaagatcg atccggcggg gcaggtgccg
     8581 ataaagttcg agatcaggtt cgacccatcc gtcatccggg cgactatgag ctatgccctg
     8641 caagcgcgca tcacggtcga cggcaagctc tggttcgtca cggatacgca ccatccggtc
     8701 gatccgctat ccgacaagcc gcagaccgtc ctggtcaaaa gggtggcgcg ctaacactcc
     8761 cacgaaacga aaaagccgag gcgttgcccc ggcttttttt gttttgaaac agatgtttcg
     8821 ttcaaaaagt
//
LOCUS       DASYCS010000387         4050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_982737,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000387 DASYCS010000000
VERSION     DASYCS010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_982737"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1000
                     /gene="ppk1"
                     /locus_tag="VGM46_12060"
     CDS             <1..1000
                     /gene="ppk1"
                     /locus_tag="VGM46_12060"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEY4193371.1"
                     /translation="AAEAGKSVTALVELKARFDEEANIRWARDLERAGVQVVFGFIEL
                     KTHAKMSLVVRREDGKLRNYVHLGTGNYHPVTARVYTDLSFFTTDPTIARDVAQLFNF
                     ITGYAEPAEEMRLAVSPFTLRKRILKHIADEVAHARAGRPARIWMKMNSLVDPTIIDA
                     LYEAGHAGVEIDLVVRGICCLRPQVPGLSENIKVKSIVGRFLEHSRIYCFGNGHGLPS
                     DDAIVYISSADLMPRNLDRRVETMVPITNPTVHEQILGQIMLGNIMDNQQSFDVLADG
                     TSRRVVLEEGEEPFNAQEYFMTNPSLSGRGDALKSHAPKRIAQFKRSKKQTTEAGG"
     gene            997..2508
                     /gene="ppx"
                     /locus_tag="VGM46_12065"
     CDS             997..2508
                     /gene="ppx"
                     /locus_tag="VGM46_12065"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEY4193372.1"
                     /translation="MISVSQGRLQDRRPLSVIDIGSNSIRLVVYEGLARSPTVLFNEK
                     MLAGLGRGIVSTGKLDPEAVTRSLEEFRRFRYLSEQAGAEHMHVLATAAAREAVNGPD
                     FIHRAEEVLGTEIRVISGREEAYYAALGVISGFKSADGISGDLGGGSLELVDVCGETV
                     GDGITLPLGGLRLQDMAGNSLAQAARIARSELARAELLKGGQGRPFYAVGGTWRNLAR
                     LHMEMTNYPLGVMHHYEIGVESAASFLRQVARGDLDKTRGIEGVSKNRRSLLPYGAIV
                     LQEIMAVMKPSKIIVSALGVREGFLYSLLDEQEQKTDPLITATEELARLRSRSVTHAH
                     ELVEWTGQTFAALGIDETEDEARYRRAACLLADIGWRAHPEYRGKQSLNIIAHASFIG
                     VDHPGRAFLALTNLFRHEGLFDGSAAELKSLATPRYLERARVLAATMRVVYLLSAAMP
                     GVIPRLKWEKQDGGGLALVLPASLAGLYGERPVGRLTQLARIVNRPLVLKVAE"
     gene            complement(2583..3005)
                     /locus_tag="VGM46_12070"
     CDS             complement(2583..3005)
                     /locus_tag="VGM46_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010915410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY4193373.1"
                     /translation="MSNSVSTAGSGSSASAPAVVLVARILLSILFIVSGYGKLTAIAG
                     TAGYFANIGMPMPTATVVIVGLVELLGGLAILVGFKTRIAAIIVALFTLAATAVAHLD
                     FSQAGNALMLQKNLGITGGLLLLAVLGAGKLSIDGRRG"
     gene            complement(3160..3705)
                     /locus_tag="VGM46_12075"
     CDS             complement(3160..3705)
                     /locus_tag="VGM46_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193374.1"
                     /translation="MAPAIADGIINRCQSPSMPGVLTNLLAALFSICEQSGLPSLKLF
                     NPRCLPVRNDSHGLLSAGNPKRNAKRIDIAAADCDAADALGVLADVSIEVGASRFRPA
                     CRGAARQCGGKNCNLKRFHHRCEHTADPRFGKAPMKTGEGTDDGHDRHGSNMSIVPTA
                     AKTVAATSRHRVMLHRPDAEL"
     gene            complement(3911..>4050)
                     /locus_tag="VGM46_12080"
     CDS             complement(3911..>4050)
                     /locus_tag="VGM46_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006332122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193375.1"
                     /translation="DNAAKIAKTAHKNGTTLREEALATGLVSEADYDRLVRPEDMTHP
                     G"
BASE COUNT          776 a         1187 c         1321 g          766 t
ORIGIN      
        1 tgcggcggaa gccggcaaat cggtgacggc gctggtggag cttaaggcgc gcttcgacga
       61 ggaagcgaac attcgctggg cgcgcgacct ggaacgggcc ggcgtgcagg tggttttcgg
      121 cttcatcgag ctgaaaaccc acgccaagat gtcgcttgtg gtgcggcgtg aggacggcaa
      181 gctgcgcaat tacgtccatc ttggcaccgg caattaccac ccggtcacgg cgcgcgttta
      241 caccgacctt tcctttttca ccaccgatcc gacgattgcg cgcgatgttg cgcagctctt
      301 caatttcatc accggttatg ccgaaccggc cgaggaaatg cgcctcgccg tatcgccctt
      361 cacgctcagg aagcgcattc tcaagcatat cgccgacgag gtagcccatg cgcgagccgg
      421 ccggccggcc cgcatctgga tgaagatgaa ttcgttggtc gatccgacca tcatcgacgc
      481 gctctatgaa gccggccatg ccggcgtcga gatcgacctc gtggtgcgcg gcatctgctg
      541 ccttcgcccg caggttccgg gcctgtccga aaacatcaag gtcaagtcca tcgtcggccg
      601 tttcctcgaa cacagccgca tctactgctt cggcaatggc catggcctgc cgtccgacga
      661 cgccatcgtt tatataagct cggcggacct gatgccgcgc aacctcgacc gccgggtgga
      721 aaccatggtg ccgattacca acccgaccgt gcatgagcag attcttgggc agatcatgct
      781 gggcaacatc atggacaacc agcaaagttt cgacgtattg gcagacggaa cctcccggcg
      841 cgtggtgctg gaggaaggtg aagagccgtt caatgcgcag gaatatttca tgaccaatcc
      901 gagcctgtcg ggaaggggtg acgcgctgaa atcgcatgcg cccaagcgca tcgcgcaatt
      961 taagcgcagc aagaaacaga ccacagaagc gggcggatga tttcagtttc ccaggggcgg
     1021 cttcaggacc gccgcccatt gtcggtcatc gatatcggtt cgaactcgat ccgcctcgtc
     1081 gtctacgagg ggcttgcgcg ctcgccaacg gtgcttttca acgaaaagat gctggccggc
     1141 cttgggcgcg gcatcgtttc gacgggcaag ctcgatccgg aggcggtgac gcgctcgctg
     1201 gaggaattcc gccgcttccg ctacctgtcg gaacaggccg gagccgagca catgcatgtg
     1261 ctggcgaccg ctgccgcgcg cgaggcggtc aacggccccg atttcatcca tcgtgccgag
     1321 gaagtgcttg gcaccgagat ccgggtgatc agcggccgtg aggaagccta ttatgcggca
     1381 ctcggcgtca tttccggttt caagtccgca gatggcatct caggcgatct tggcggcggc
     1441 agccttgagc tggtcgatgt ctgcggcgaa acggtgggcg acggcatcac gttgccgcta
     1501 ggcgggctgc ggcttcagga catggccggt aattcgctcg cccaggcggc gcgtatcgcg
     1561 cgcagcgaac tggcgcgcgc ggaactgctg aagggcgggc agggcaggcc tttttatgct
     1621 gtcggcggca cttggcgaaa cctggcgcgc ctgcacatgg agatgacgaa ctatccgctc
     1681 ggcgtcatgc atcattacga gatcggggtg gaaagtgcgg ccagcttcct gaggcaggtg
     1741 gcgcggggcg acctcgacaa gacgcggggt atcgagggcg tatcgaagaa ccgccgttcg
     1801 ctgttgccct atggcgccat cgttttgcag gaaatcatgg cggtgatgaa gccgtcgaag
     1861 atcatcgtct cggcgctcgg cgttcgcgaa gggttccttt attcgctgct ggacgagcag
     1921 gagcaaaaga ccgacccgct gattaccgcg accgaggaac tcgcccgcct gcgttcccgg
     1981 tccgtcacgc atgcgcacga acttgtggag tggaccggcc agaccttcgc cgcccttggc
     2041 atcgacgaga cggaagatga ggcgcgttac cgccgggccg cctgcctgct ggccgatatc
     2101 ggctggcgcg cgcacccgga atatcgcggc aagcaatcgc tcaacattat tgcgcacgca
     2161 tcgttcatcg gggtggatca tcccggccgc gccttcctgg cgctgaccaa cctgtttcgc
     2221 catgaagggc tgttcgacgg gtccgccgcc gagctgaagt cgcttgccac gccgcgttat
     2281 ctggagcgcg cccgcgtgct ggcggcgacg atgcgggtcg tttacctgct gtcggcggcc
     2341 atgccgggcg tcattccacg gctgaagtgg gaaaagcagg atggtggcgg gctagcgctg
     2401 gttctgccgg cttcgctcgc cgggctttac ggggagcgcc cggttggccg cctcacgcaa
     2461 ctggcgcgca tcgtaaaccg cccgctggtg ctgaaagtgg ccgaatagga tattcgtaac
     2521 gggcggaagc cgcaaaaaaa gcccggattg ctccgggctt ttttgtttca tgcaaggctt
     2581 tatcagcccc ggcgaccgtc gatcgacagc ttgccggcgc caagcacggc gagcagcagc
     2641 agaccgccgg tgatgccgag gttcttttgc agcatcagcg cattgcctgc ctgcgaaaaa
     2701 tcgaggtggg cgacagccgt cgcggccagc gtgaagagcg cgacgatgat ggcggcgatg
     2761 cgggttttga agccgaccag aatggcaagg ccgcccagaa gttcgacaag gccgacaata
     2821 accaccgtcg cggtcggcat cggcatgccg atattggcga aataaccggc ggtgccggca
     2881 attgcggtca atttgccgta gccggagacg atgaacagga tggacaacaa tatgcgcgcg
     2941 accaacacca cggccggagc gctggcagag gagccggaac cggcggtgga aactgaattg
     3001 gacatgcgat ctctccaggg ttgaatgaat tcaatatata gaagatcgat tgttcaccaa
     3061 agacccaatg taacgacaca tcgttcagcg aaatgatgga tttgtgatcc gctatgcgtt
     3121 tcgggcgttg tcgaattatt ggaccggtcg ccaggcgcgt caaagttccg catctggccg
     3181 atggagcata actcgatgcc ggctggtggc ggcgaccgtc ttagcggcgg tcgggacgat
     3241 tgacatattg gacccgtggc ggtcatggcc gtcatcggtt ccttccccgg ttttcatcgg
     3301 cgccttgccg aagcgcggat cggcggtatg ttcacagcgg tgatgaaaac gcttcagatt
     3361 acaattcttg ccgccgcatt ggcgggctgc gcctcgacag gcgggccgga aacggcttgc
     3421 gccaacctca atcgagacgt cggcgagaac gccaagggca tcagccgcgt cgcaatcagc
     3481 cgcggcaatg tcgatgcgct tagcgttccg ttttgggttc ccggcggaaa gaaggccgtg
     3541 actgtcatta cggacaggca gacagcgagg attgaaaagc ttcaggctag gcaaaccact
     3601 ttgctcgcag atcgagaaca aagctgccag tagattggtg agaacgccgg gcatcgacgg
     3661 tgactggcat cgattaataa tgccgtcggc gatggctgga gccaaggtca ggcgtcttgg
     3721 tcacatgcgc cgccaggcag gcggtacgcc gcaagaccgg tcggacgatg cgccgcgatc
     3781 tgcccggccc gattggtagc gacggcggca tttccgttcc ggaaggagaa taggtcgact
     3841 cacgggggcg ttggcgtgcg tcaatcggag cgatagaccg aagcggtccg tgtcggtgag
     3901 gtgtggcgct ctagccggga tgggtcatgt cttccggccg caccagccgg tcatagtcgg
     3961 cctcgctgac caggccggtg gccaatgcct cctcgcgcag ggtcgtgccg ttcttatgcg
     4021 ccgtcttggc gatcttggcg gcgttgtcgt
//
LOCUS       DASYCS010000388         4831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2142752,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000388 DASYCS010000000
VERSION     DASYCS010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4831
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2142752"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..372)
                     /locus_tag="VGM46_12085"
     CDS             complement(85..372)
                     /locus_tag="VGM46_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3175 domain-containing protein"
                     /protein_id="HEY4193376.1"
                     /translation="MAAKHKWSADVTEHSDALDLEEHIFKSDDPKKIADSLKRSAEHS
                     AKRKAEPFQSAMSMLNFYINRAGKNLSAKREKVLEDAKDELRAAFGRDKED"
     gene            581..1804
                     /locus_tag="VGM46_12090"
     CDS             581..1804
                     /locus_tag="VGM46_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4193377.1"
                     /translation="MNFSTQAGTRADIDGRYAWTRLAVSLLLGTIGGVGMWAVVIVLP
                     AVQLEFGVDRADASLPYTATMVGFALGNVIVGRAIDRVGYWVPALVTSILLGLGFLLA
                     STATSILQFSLVQGVMIGLGTAATFGPLMADISHWFDRRRGVAMAIAAAGSYLAGTFW
                     PAVMPGLMKAEGWRFTYEVIGVICLLGMTPLTLMLRRRPPHAMAGEGGRKIRPISLSP
                     TALQVLLVIAGLGCCAAMSMPQVHIVAYCMDLGYGVARGADMLALMMAAGIVSRVASG
                     FIADRIGGIKTLLIGSVLQCLALFFYIPFDGLASLYVVSLIFGLSQGGIVPCYAVIVR
                     EYMPAAEAGQRVGVVIMATILGMALGGWMSGWIYDLTGSYTAAFLNGIAWNLLNIVIM
                     VLVLWSARRASLAAS"
     gene            complement(1913..2362)
                     /locus_tag="VGM46_12095"
     CDS             complement(1913..2362)
                     /locus_tag="VGM46_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEY4193378.1"
                     /translation="MLTKKGKYGLKALVHLSGLPAGQLAFVNDIAVTNHIPKKFLDAI
                     LGELRNAGFVQSRKGKGGGYRLARPASEIKIGHVIRVLDGPLAPIPCASRTQYQHCED
                     CDEATCKVRHMMLEVRHAIAEVLDNRSLAALRDADNDDYPLGLASHR"
     gene            complement(2520..3506)
                     /locus_tag="VGM46_12100"
     CDS             complement(2520..3506)
                     /locus_tag="VGM46_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010973096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0043199 - sulfate binding [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY4193379.1"
                     /translation="MKRLLLAATAAAGLLLSSASAWSADKLLNASYDVGRELFVAVNK
                     AFIATHPGVTIDQSHAGTSKQARAIVEGLAADVVTFNQVPDIDFLVKNGYVAADWQKK
                     FPDNASPFYSLPSFLVRAGNPKHIKDWDDLARDDVQIIFPNPKTSGNARYTYLAAYAY
                     GLEKFNGDAAKVNEFVGKIFAHVPVFDTGGRASTTTFAERGIGDVLITFESETQGIAK
                     EYGTDKYETVTPSVSLLAEFPVAVVDKVAKDNGTGDLAKSYLDFLYTPEGQKIEAENS
                     LRVRDEKVAAEFKDKFPPVRLVTVEDVFGGWAKVQKEHFAEGALLDQIYGKR"
     gene            complement(3656..4699)
                     /locus_tag="VGM46_12105"
     CDS             complement(3656..4699)
                     /locus_tag="VGM46_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HEY4193380.1"
                     /translation="MEVRVANVRKEFDTFAALHDVSLDIRSGELIALLGPSGSGKTTL
                     LRLIAGLEQPTVGKIYFGDEDASERTIRERNVGFVFQHYALFRHMTVADNVGFGLKVR
                     PGATRPPAAEIRRRASELLDLVQLSGLEKRYPAQLSGGQRQRVALARAMAIEPKVLLL
                     DEPFGALDAQVRRELRRWLREIHDATGHTTVFVTHDQEEALELADRVVVMSQGRIEQV
                     GTADDVYDTPNSPFVYGFIGESSSLPVKVEDDQLWIADRLIGLSAQGAPAGEATLFFR
                     PHDVELVDGCSGCIAGTVAASRRVAGTRRVELEIGGHRQRVEIELPVDHPAAQQSRVA
                     FRPRQWKVFPTAT"
     gene            complement(4712..>4831)
                     /gene="cysW"
                     /locus_tag="VGM46_12110"
     CDS             complement(4712..>4831)
                     /gene="cysW"
                     /locus_tag="VGM46_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171435.1"
                     /note="Part of the ABC transporter complex cysAWTP
                     involved in sulfate/thiosulfate import; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HEY4193381.1"
                     /translation="AAGAFAVASLLAGLALVTLALKTLLEMRYGAEIAATRGH"
BASE COUNT          816 a         1397 c         1577 g         1041 t
ORIGIN      
        1 ttctttggcc gcatttccgg acgcaaaacc gtttcacact cttgctggaa atactccagg
       61 gcgaacggcg gttgcgtcgg gaggtcagtc ttccttgtcg cggccgaaag ctgcacgcaa
      121 ttcgtccttg gcgtcttcca gtactttctc gcgcttggct gacaggttct tgccggcgcg
      181 gttgatatag aaattcagca tcgacatggc cgactgaaac ggctcggcct tgcgctttgc
      241 gctgtgttca gcggaacgtt tgagggaatc cgcgatcttt tttggatcat cggacttgaa
      301 aatatgctct tcgaggtcga gcgcgtcgct gtgttcggta acgtccgccg accatttgtg
      361 cttcgctgcc attatcatct cccttagata atttcctttg ggtcattgcc tttgaccgag
      421 tgccggcaaa acgccgattg gggcggcgcg ttccgcgccg atcgcaaata gcagtgcttt
      481 cgacgtctgt ggttccatag gccgggtgcg gtggtgctga ccaacaagcc gccgggttgt
      541 agaattgggt cgcgcatgcc gcaggcgggg aggtcatgac ttgaattttt ctacgcaggc
      601 cggcactcgg gcagacatag acggtcgcta tgcctggacg cgacttgccg tctcgctgct
      661 gctgggcacc atcggcggcg tcggcatgtg ggcggtcgtc atcgtgctgc cggcggtgca
      721 gctcgagttc ggcgtcgacc gggcggatgc atccttgccc tatacggcaa ccatggtcgg
      781 ctttgctctt ggcaacgtca tcgtcgggcg tgcaatcgat cgtgtcggct actgggttcc
      841 cgctttggtc acctcgatat tgctgggtct cgggttcctg cttgcttcga ccgcgacatc
      901 cattctccag ttctcgctgg ttcaaggggt gatgatcggc ctgggaacgg cggcgacctt
      961 cggtccgctg atggccgata tctcgcattg gttcgatcgc cgccgaggcg tcgccatggc
     1021 gatagccgcc gccggtagct atcttgccgg aaccttctgg ccggccgtaa tgccgggctt
     1081 gatgaaggcg gaaggctggc gtttcaccta tgaggtgatc ggcgtgatct gccttttggg
     1141 catgacgccg ctgacgctga tgctgcggcg gcggccaccg cacgcgatgg cgggcgaggg
     1201 aggccggaaa atccggccga tttcgctgtc gccgacagcc ctgcaagtcc tgctggtcat
     1261 cgccggcctc ggctgctgcg cggccatgtc gatgccgcag gtgcatatcg tcgcctattg
     1321 tatggacctc ggctatggcg tggcgcgcgg cgccgacatg ctggcgctga tgatggcggc
     1381 ggggattgtc agccgcgtcg cgtcggggtt catcgccgac cgcatcggcg gcatcaagac
     1441 gcttttgatc gggtcggtgc tgcaatgcct ggcgttgttt ttctacatac ccttcgacgg
     1501 acttgcttcg ctctatgtcg tgtcgttgat attcggattg tcgcagggcg gcatcgtgcc
     1561 ctgctatgcg gtcatcgtgc gcgaatacat gccggcggcc gaagccggcc aacgggtcgg
     1621 cgtcgtcatc atggcaacca ttctcggcat ggcgctgggc ggctggatgt ccggctggat
     1681 ctacgacctg accggttcct acaccgccgc tttcctcaac gggatcgcgt ggaacctgtt
     1741 gaacatcgtc atcatggtgc ttgtgctgtg gtcggcgcgg cgcgcttcgc tcgcggcgtc
     1801 gtaaggggct agagcggttc cgatttttgc tgaatcgaca aaaccgctct ttctctttgt
     1861 tttggctgca cttccggacg caaaaccgtt ttacgctttt gctgcaaatg ctctagcggt
     1921 gcgaggccag cccgagcggg tagtcgtcat tgtcggcgtc gcgcaacgcg gcaaggctgc
     1981 ggttgtcgag cacctcggca atggcgtggc gaacttccag catcatgtgg cggaccttgc
     2041 aggtggcctc gtcgcaatct tcgcagtgct ggtattgggt gcggctggcg caggggatcg
     2101 gcgccagcgg gccgtcgagg acgcgaatga catggccgat cttgatctcg gaggccggcc
     2161 gcgccagccg gtagccgcca cccttgccct tgcggctctg gacgaagccg gcattgcgca
     2221 attcgcccaa aatggcgtcg aggaatttct tcggaatgtg gttggtgacg gcgatgtcgt
     2281 tgacaaaggc caactgcccg gccggcaatc cggaaagatg cacgagggcc ttgaggccgt
     2341 atttgccttt tttggtgagc atggccgaga cgcttcattt tccggatttg tagaccggca
     2401 gttcatccac attacagata tagtgtcaaa agcgttgatt ttccggcaat ttccggcccg
     2461 tgacagaagg aaaagcccgg cgtgaggccg ggcttttctt tggctgtgac ggccaaggct
     2521 tagcgtttgc cgtagatctg gtcgagcaat gcgccttcgg cgaaatgctc tttctgcact
     2581 tttgcccagc cgccgaacac atcctcgacg gtgacgaggc ggaccggcgg gaacttgtcc
     2641 ttgaattcgg ccgccacctt ctcgtcgcgg acgcgcagac tgttctcggc ctcgatcttc
     2701 tggccttccg gcgtgtagag gaaatcgagg tagctcttgg caaggtcgcc ggtgccgttg
     2761 tctttggcca ccttgtcgac gacagcgaca gggaattcgg cgagcaggct gaccgaagga
     2821 gtgacggtct cgtatttgtc ggtgccgtat tccttggcga tgccctgggt ttcggattcg
     2881 aaggtgatca gcacgtcacc gatgccacgc tcggcgaagg tggtggtcga ggcgcggccg
     2941 ccggtgtcga agaccgggac atgcgcgaag atcttgccga cgaattcatt gaccttggcg
     3001 gcgtcgccgt taaatttttc caatccgtag gcgtaagcgg cgagataggt gtagcgcgca
     3061 ttgccggagg tcttcgggtt agggaagatg atctgcacgt catcgcgggc cagatcatcc
     3121 caatccttga tatgcttcgg gttgccggca cgcaccagga aggacggcag cgaatagaag
     3181 ggcgaggcgt tgtcggggaa cttcttctgc cagtcagcgg cgacatagcc gttcttgacc
     3241 aggaaatcga tgtcgggaac ctgattgaag gtgaccacgt cagccgccag gccctcgacg
     3301 atggcgcgcg cctgcttcga ggtgccggca tgcgactggt cgatggtgac gcccggatgc
     3361 gtcgcgatga aagccttgtt gacggcgacg aacaattcgc gcccgacatc gtaggatgcg
     3421 ttgagcagct tgtcagccga ccaggccgag gcggaagaca aaagcagacc ggctgcggcg
     3481 gtagcggcaa gcaaaaggcg tttcatggaa ggctccggtt cgagagttgc cggaccatat
     3541 agccgggagt cgaccgcgcc cgcgaggcat ggcgcggcgg taggttgatg tttgtaggaa
     3601 atccgttctc aaaaacggat tctttcgggt attctgttcc ttacccttgc catcattacg
     3661 tggcggttgg gaaaaccttc cactggcgcg ggcggaaagc gacgcggctc tgttgcgcgg
     3721 cagggtggtc taccggcagt tcgatttcca cgcgctgtct gtggccgccg atttccagtt
     3781 cgacgcgcct tgttccggca acgcggcggc tggcagcgac ggtgccggcg atgcagccgg
     3841 agcagccgtc gaccagttcc acatcgtgcg ggcggaagaa caaggttgcc tcgccggcgg
     3901 gcgcgccctg cgctgaaagg ccgatgaggc gatcggcgat ccatagctgg tcgtcctcga
     3961 ccttgaccgg caaggaactg gactcgccga tgaagccata gacgaaaggc gagttcggcg
     4021 tgtcgtagac atcgtcggcg gtgccgacct gctcgatgcg gccctggctc atgacgacga
     4081 cgcggtcggc tagttccagc gcctcttcct ggtcgtgggt gacgaacacg gtggtgtggc
     4141 cggtggcgtc gtgaatttcg cgcagccagc ggcgcagttc acgccgcacc tgcgcgtcga
     4201 gcgcgccgaa aggctcgtcg agcagtagca ccttcggctc tatggccatg gcgcgggcca
     4261 gtgcgacgcg ttggcgctgg ccgcccgaaa gctgcgccgg atagcgcttt tccagacctg
     4321 aaagctgtac gagatcgagc agttcggaag cgcggcggcg aatttccgcc gccggcggcc
     4381 gcgttgcgcc gggccgcact ttcaggccga agccgacatt gtcggccacc gtcatatgcc
     4441 gaaacagcgc gtaatgctgg aacacgaagc cgacattgcg ttcgcggatg gtgcgttccg
     4501 aagcatcctc gtcgccgaaa tatatcttgc cgacagtcgg ctgctcaagg ccggctatca
     4561 gccgcagcag cgtcgtcttg ccggaaccgg acggccccag cagggcgatc agctcacccg
     4621 acctgatgtc gagcgacacg tcgtgcaggg cggcaaacgt gtcgaattcc ttgcgtacat
     4681 tggcaacgcg aacttccatg aaaaagacct ctcaatgtcc gcgcgttgcg gcgatttccg
     4741 cgccgtagcg catttcaagc agggttttca acgccaaggt caccagcgcc agcccggcca
     4801 gcagcgaagc cacggcgaag gcgccggccg c
//
LOCUS       DASYCS010000389         2265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1560515,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000389 DASYCS010000000
VERSION     DASYCS010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1560515"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="VGM46_12115"
     CDS             complement(<1..580)
                     /locus_tag="VGM46_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEY4193382.1"
                     /translation="MPETFKAILVSRDADKKQTVAVTDLTEAELMEGNVMVAVEATTI
                     NYKDGLAITGKAPVVRRWPLIPGIDFAGTVISSNHPDWRKGDGVILNGWGVGETHHGA
                     FAGRARVNGDWLVPLPEGMSAHDAMAVGTAGYTAMLCVMVLERHGIVPERGPVVVTGA
                     AGGVGSVATSLLSSLGYHVIASTGRGAQSGYLL"
     gene            complement(600..1079)
                     /gene="lspA"
                     /locus_tag="VGM46_12120"
     CDS             complement(600..1079)
                     /gene="lspA"
                     /locus_tag="VGM46_12120"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEY4193383.1"
                     /translation="MKRFLPFALLAIAAIALDQWIKYLVETGLALHELVDVLPFLALY
                     RTYNTGIAFSMLASFGDVGLIVVSVCVVAFVLYLAARTAPHQFVARIGFALIVGGALG
                     NLIDRAVYGHVIDYILFHTPVWSFAVFNLADAFITVGAVAVVFDEFIGWRREPPSAG"
     gene            complement(1076..1936)
                     /locus_tag="VGM46_12125"
     CDS             complement(1076..1936)
                     /locus_tag="VGM46_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEY4193384.1"
                     /translation="MNEHEGRRAVGQVKEITSLANPLIKDIKALALKKFRDQQHAFMA
                     EGLKLVIDALDLGWSIRTLIFAKAGLGNAAVEKVAARTVASGGTVLEVSEKVLSAITR
                     RDNPQMVVGVFAQKMQALKDIHAKPGDVWVALDRVRDPGNLGTIIRTVDAVGAKGVIL
                     VGDTTDPFSVETVRATMGSIFAVPVARTTPEAFLAWRENFPGLVAGTHLKGAVDYRSV
                     DFSRGPVLLLMGNEQQGLPDALAESCDRLLRIPQAGRADSLNLAVATGVMLFEIRRAA
                     LKLGPEEAHP"
     gene            complement(1959..>2265)
                     /locus_tag="VGM46_12130"
     CDS             complement(1959..>2265)
                     /locus_tag="VGM46_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEY4193385.1"
                     /translation="RYDIILFDPPAYGRGPKGEVWQLFEDLPALTDICRAILTPKPLA
                     VVLTAYSIRSSFFAIHALMRDTFAGMGGTVESGELIIREKSAGRALSTSLFSRWVAP"
BASE COUNT          436 a          709 c          737 g          383 t
ORIGIN      
        1 caagcaggta gccgctttgc gcgccgcgcc cggtcgaggc gatgacatgg tagccgaggc
       61 tggacagaag tgaggtggcg acggagccga cgccgccggc agcgcccgtc accaccaccg
      121 ggccacgctc cggcacgatg ccgtgccgct ccagcaccat tacgcacagc atggcggtat
      181 agcctgcggt accgacggcc atggcgtcat gcgcgctcat gccttcgggc agcggcacca
      241 gccagtcgcc attgacgcgg gcgcggccgg caaaggcgcc gtgatgcgtt tcgccgacgc
      301 cccagccgtt caggatgacg ccgtcgccct tgcgccaatc gggatggttg gaggaaatga
      361 cggtgcctgc gaaatctatt ccgggaatta gcggccagcg gcgcactacg ggggccttgc
      421 cggtgatggc gaggccgtcc ttgtagttga tggtcgtcgc ctcgacggcg accatcacat
      481 tgccttccat caactcggcc tcggtgaggt cggttaccgc gaccgtctgc ttcttgtcgg
      541 catcgcgcga aacgaggatg gccttgaagg tttccggcat ttttctctcc ccgtattggt
      601 cagccagccg atggcggctc gcgccgccag ccgatgaatt cgtcgaacac caccgcgacg
      661 gctccgaccg ttatgaacgc gtcggcgagg ttgaacacgg caaacgacca gaccggcgtg
      721 tggaacagga tatagtcgat gacgtgacca taaacggcgc ggtcgatgag gtttcccagc
      781 gcgccgccga cgatcagcgc aaagccgatc ctggcgacaa actggtgcgg cgcagtgcgc
      841 gcggcgaggt aaagcacgaa ggcgacgacg cagaccgaaa cgacgatcag cccgacatcg
      901 ccaaatgatg ccagcatgga gaaggcgatg ccggtgttgt aggtgcggta aagcgccaga
      961 aagggcagga cgtcgaccaa ttcatgcaag gcaaggccgg tttcgaccag atattttatc
     1021 cattggtcaa gcgcgatggc ggcgatggcc aaaagggcga atggcaggaa gcgtttcacg
     1081 gatgcgcctc ttccgggcca agcttcaggg cggcgcggcg gatctcgaac agcatgacgc
     1141 cggtggcgac cgccagattg agtgaatcgg cgcgtccggc ctgcggtatg cgcaagagcc
     1201 ggtcgcagct ttccgccagc gcatcgggca ggccctgctg ctcattgccc atcaacagca
     1261 gcaccggccc ccggctgaaa tcgacggacc gataatccac cgcccccttc agatgcgtgc
     1321 cggcgacaag gcccggaaaa ttctcacgcc acgcaagaaa ggcctccggg gtggtcctgg
     1381 cgaccggaac ggcaaagatc gaacccatcg tagcgcgcac cgtctctact gaaaacggat
     1441 cggtggtgtc gcccaccaga atgacgccct tggcgccgac cgcatcgacg gtgcggataa
     1501 tggtgccgag attgccgggg tcgcgcaccc ggtcgagcgc cacccagaca tcgccgggct
     1561 tggcgtggat atccttcagc gcctgcattt tctgggcaaa gacgccgacc accatttgcg
     1621 gattgtcgcg gcgcgtgatg gccgacagca ccttttccga tacttccagc accgtgccgc
     1681 cgctggccac ggtgcgggcg gcgacctttt cgaccgccgc attgccaagc cccgccttcg
     1741 caaagatcag cgtcctgatg gaccagccga gatcgagcgc gtcgatgacc agtttcagcc
     1801 cttcggccat gaaggcgtgc tgctggtcgc ggaatttctt caacgccagc gccttgatat
     1861 ccttgatcag cggattggcg aggctggtga tttccttgac ctggccgacc gcgcggcggc
     1921 cttcatgctc gttcatttgt cggtccgttt tttcgcggtc atggcgcgac ccagcgcgag
     1981 aacagcgagg tggaaagcgc acgcccggcg gatttttcgc ggatgatcag ttcgccggat
     2041 tcgaccgtgc cgcccatgcc ggcaaacgta tcgcgcatca aggcgtggat ggcgaagaag
     2101 gacgagcgga tcgaataggc ggtcagcacc acggccagcg gcttcggcgt caggatggcg
     2161 cggcaaatgt cggtcaaggc cggcaaatcc tcgaacagct gccagacttc gcccttgggg
     2221 ccgcggccat aggcgggcgg gtcgaacagg atgatgtcgt aacgg
//
LOCUS       DASYCS010000390         2064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4168148,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000390 DASYCS010000000
VERSION     DASYCS010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4168148"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="VGM46_12135"
     CDS             complement(<1..882)
                     /locus_tag="VGM46_12135"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY4193386.1"
                     /translation="MAQNPMSVLIVGAGPTGLTAALELARRGIICRIIDAEDGPTPLS
                     KAVGISAHSLDILEASKVTEKLLARGLKIRHAHFHFEGRELGILDFSILPHRHNFLLS
                     LPQSVTETAMAEVLAGFGVEVEWSTRFEGLTEQDDAVNVRMATTAGEQTSRFDYVFGA
                     DGTKSRVREAIGADFAGYTHKRQWSIADAEVSEWPYEPGSAQAFLNRNGDVGFIIPIG
                     ENRFRAVSNTADAMALVPGAYKIKQVLRTDRFHIPARQASFYQTRRVFLGGDAAHAHS
                     PIGARGMNLGIEDAASFA"
     gene            complement(1043..1759)
                     /locus_tag="VGM46_12140"
     CDS             complement(1043..1759)
                     /locus_tag="VGM46_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4193387.1"
                     /translation="MAKTPLLKVAGISAGYGGMPVLRDVSLEVQPAEIVALVGGNGAG
                     KTTLLRALSRVIACTGGIEFDGRDIVSMTSDEAFGAGLVQVPEGRQLFDRMTVEDNLL
                     MGAYRRGDKAAIARDLDRMYGLFPRLGERKRQLAGSMSGGEQQMCAMARGLMAAPKLL
                     MIDEMSLGLAPVVVEQLMEVLATIRSEGVTILLVEQDVHLALSGADRGYVMETGHIVH
                     QGAAKALIDDPEVRRAYLGL"
     gene            complement(1774..>2064)
                     /locus_tag="VGM46_12145"
     CDS             complement(1774..>2064)
                     /locus_tag="VGM46_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004687607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4193388.1"
                     /translation="RLELAKALAMQPKLLLCDEVMAGLNHVEIDEVIEVIRKVRGEGI
                     SVLVIEHVIKAIKSLSDRLMVLHHGEKIADGAPDTVLSDKTVVEAYLGKRRA"
BASE COUNT          388 a          657 c          609 g          410 t
ORIGIN      
        1 ggcgaacgaa gcggcgtctt cgatgccgag gttcatgccg cgcgcaccga tcggcgaatg
       61 cgcgtgggcc gcgtcgccgc cgagaaagac gcgccttgtc tgatagaatg aggcttgtcg
      121 tgccgggatg tgaaagcgat cggtacgcag aacttgcttt attttatagg caccgggcac
      181 cagcgccatc gcgtccgctg tattggacac ggcgcgaaaa cggttctcgc caatcggaat
      241 aatgaaaccg acatcgccat ttcggttaag gaaggcttgg gccgagcctg gctcgtaagg
      301 ccattccgat acctcggcat cggcgatgct ccactgacgt ttgtgcgtgt atccggcgaa
      361 gtcagcgccg atcgcctcgc gtacgcggct tttggtcccg tcggctccga aaacataatc
      421 gaagcgcgat gtttgttcgc cggcggtcgt cgccatccgt acattgacgg cgtcatcctg
      481 ttcggtgagg ccctcgaacc gcgtggacca ttccacctcg accccaaaac ctgcaagcac
      541 ctcggccatc gccgtttccg tgacgctctg gggcagggac agcaggaaat tgtggcggtg
      601 cggcaatatc gaaaaatcga gaatgccgag ttcgcggccc tcgaaatgga aatgggcgtg
      661 cctgatcttg agacccctgg cgagcagctt ttccgtgacc ttggatgctt cgagaatatc
      721 aaggctatgg gcgctgatgc caaccgcctt gctgagcggg gtcggcccgt cctcggcatc
      781 gattatgcgg caaattatgc ctcgcctcgc cagttcgagc gcggccgtca ggccggtcgg
      841 accagcgccg acgataagaa cggacatggg attttgtgcc atggaaaatc ataacagctt
      901 ttaggtgaag cgtaagcaac gacgtcgcag tcgatcacac gctcgcttcc ttattgccgc
      961 ttcctcagtc cgaccgcctg aacagttggc aggcccaggc gtttgccgtt gcggtcaatc
     1021 tggatgagcc ggcattcgcc gctcatagcc ctagataggc gcggcgcact tccggatcgt
     1081 cgatcagcgc cttggcggcc ccttgatgga cgatgtggcc ggtttccatg acataaccgc
     1141 ggtcggcgcc ggacagggcc agatgcacgt cctgttcgac cagaagtatc gtcacgcctt
     1201 cgctgcgaat cgtcgccagt acttccatca actgctcgac cacgacaggc gccaggccaa
     1261 ggctcatttc atcgatcatc agcaatttgg gggccgccat caggccgcgc gccatggcgc
     1321 acatttgctg ctcgccgccg gacatcgatc cggccagctg gcgcttgcgc tcgccgagcc
     1381 tcggaaaaag cccgtacatg cggtcgagat cgcgcgcaat cgccgccttg tcgccacgcc
     1441 ggtaagcgcc catcaggaga ttgtcctcga ccgtcatccg gtcgaaaagc tggcgtccct
     1501 ccggcacctg caccagcccc gcgccgaaag cctcgtcgga tgtcatggac acgatatcgc
     1561 gtccgtcgaa ctcgatgccg ccggtgcagg cgattacgcg tgacaaggcc cgcagcagcg
     1621 tcgtcttgcc ggccccgttg ccgccgacca gcgcgacgat ctcggctggt tgaacttcca
     1681 gcgatacgtc gcgaagcacc ggcatgccgc cataaccggc actgatgccg gcgactttca
     1741 aaaggggcgt tttcgccata tcggccgccg cgctcatgcc cgcctcttgc cgagataggc
     1801 ctcgaccacc gttttgtctg aaagcacggt gtccggtgcg ccatcggcga ttttctcgcc
     1861 atggtgcaga accatcagcc ggtccgaaag gctctttatg gccttgatga cgtgttcgat
     1921 gaccagcacc gaaatgcctt cgccgcgcac cttgcggatg acctcgatga cctcgtctat
     1981 ctcgacatgg ttcaagccgg ccatcacctc gtcgcacagc agaagcttcg gctgcatggc
     2041 gagcgccttg gccagttcga ggcg
//
LOCUS       DASYCS010000391         2792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3590284,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000391 DASYCS010000000
VERSION     DASYCS010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3590284"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..838)
                     /locus_tag="VGM46_12150"
     CDS             complement(59..838)
                     /locus_tag="VGM46_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HEY4193389.1"
                     /translation="MKAYPERQISRAAGWSRRAGVFSAVLLLTVWLGHHFELVETGAY
                     LWVLALVALLAAIALLLAGLAFSRLWNFGDRGGRNLTVGALSALLVLTPYAFAAYRVT
                     STPPLRDISTDPGDPPTLAATGSRTPPTPGEQRLQRESYPLVTGRNYDLPLDSVVSAV
                     DAVLKRQGWQRAEPLPVTGGQSEVTIAAVAKSFILDLPADVAIRLADDGETTLVDMRS
                     ASRYGRYDLGDNASRIVAFMAELDNEVAGELAGQVGPAPAE"
     gene            1076..1432
                     /locus_tag="VGM46_12155"
     CDS             1076..1432
                     /locus_tag="VGM46_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006330738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193390.1"
                     /translation="MEAQIDDKTLENTLAESLAEIVPDDRSVTDDAFVEVVTGALEAV
                     NGTLLFKMRISNDGQDVHVAAASIGEGNRRQFLLLTLPAGGGALKVETISKSQNPVAG
                     IAAAYAGLMDVFKAAA"
     gene            complement(1583..2341)
                     /locus_tag="VGM46_12160"
     CDS             complement(1583..2341)
                     /locus_tag="VGM46_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY4193391.1"
                     /translation="MRLFICVFAAILACAGTAFAKTPADIVEPTLHVAPGGTAGPRVA
                     LTFDACMGKVDARILSALVDNKLPATIFVTGRWLKYNQHAFAQLKAHPELFEIQNHGA
                     MHVPAVDRSMKIYGIAAAGSPQAVAAEVEGGAAALRTAGGADPRWFRGSTARYSRSAI
                     KQIRGLGFRVAGYSVNGDGGSLLGAKMTEKRIADAHDGDVVIAHINQPTHAAGSGVVK
                     GILDLKAKGYSFVKLSDAEAEGSDGTTAPVNPGS"
     gene            complement(2477..>2792)
                     /locus_tag="VGM46_12165"
     CDS             complement(2477..>2792)
                     /locus_tag="VGM46_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010914919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HEY4193392.1"
                     /translation="TQTTFGISRDDAASFLETYRQKGIVEQDPFVSIDVDGVGELMRM
                     AASKGRSVRPEIKLGICGEHGGDPASVHFCEEVGLDYVSCSPYRVPIARLAAAQAAVK
                     KA"
BASE COUNT          549 a          866 c          861 g          516 t
ORIGIN      
        1 gttgtcgcca ccgagggcga tctgtcgctt gatggggttt tcctgccagt ttgacggctt
       61 attccgccgg tgctggtccc acttgcccgg caagttcgcc ggcgacttca ttgtccagct
      121 cggccatgaa tgcgacgatg cgggaagcat tgtctcccag gtcgtagcgg ccatagcgcg
      181 aggccgaacg catgtcgaca agcgtcgttt cgccatcgtc ggcgaggcgg atagccacat
      241 cggcgggcag gtcgaggatg aagcttttcg ccacggcggc aatcgtcact tcgctttggc
      301 caccggtgac gggcaatggt tcggcgcgtt gccaaccctg gcgcttcaaa acggcatcga
      361 ccgcgctgac gacgctatcg agcggaaggt cgtaattgcg tcccgtcacc agaggatagc
      421 tttcgcgctg tagcctctgc tcgccgggtg tcggaggcgt ccggctcccg gtggcggcaa
      481 gagtgggcgg atcgccggga tcggtcgaga tatcgcgcaa aggcggcgtg ctcgtcaccc
      541 gataggccgc aaaagcatag ggcgtgagca ccagcagcgc agacagcgcg cctacggtga
      601 gattgcggcc gccacggtcg ccgaagttcc acagccgcga aaaagccagg cccgccaata
      661 aaagggcgat ggcggccaga agcgccacca gggccagcac ccagagatag gcgcccgttt
      721 ccaccagttc gaaatgatgg ccgagccaaa ccgtcagcag cagcacggcc gaaaataccc
      781 cggcccgccg cgaccagccg gctgcccgcg aaatctgtcg ctcaggatag gctttcatac
      841 gacctcgcct caccgaacct tatcgaggct tagagcgttt cggtatcgaa ttgaagccga
      901 aagcacacca ttcgacgcca tagacggcgc tatcgttcgt tgggcgaaaa aggcgtgaat
      961 ttgtccggca actttaacga atccaccgat gcacccggcc aaacgcctta atccagccat
     1021 cgtgagcgta aattctgggc gtgacgggac gcgggcgaaa tggatgaggt ttcgcatgga
     1081 agcgcagatc gacgacaaaa cccttgagaa cacactcgcc gagagcctgg cggagatcgt
     1141 tccggacgac cggagcgtta ccgacgacgc gtttgtcgag gtggttaccg gcgcgctcga
     1201 agcggtcaat ggcacgctgc tgttcaagat gcgcatcagc aatgacggtc aggatgtgca
     1261 tgtcgcggcc gccagcatcg gcgaaggcaa tagacgccaa ttcctgctgc tgaccctgcc
     1321 tgccggcggc ggtgccttga aagtggaaac gatatcgaaa agccaaaacc cggtggcggg
     1381 aattgccgca gcctatgccg ggctgatgga cgttttcaag gccgccgcct agagcgattc
     1441 cgattctaac cgaatcgaca aaaccgctct atctgtttgt tttggccaca tttccggacg
     1501 caaaaccgtt tcatactttt gctggaaatg ctctagccgg cttcatacag tccggcaccc
     1561 ttgtcttgta cgaaccgctg catcacgaac ccggattaac cggggcggtg gtgccgtccg
     1621 aaccttcggc ctcggcatct gacagcttga cgaaggaata gcctttcgcc ttgaggtcga
     1681 ggatgccctt caccacgccg gagcctgcgg catgggtcgg ctggttgata tgggcgatga
     1741 cgacatcgcc gtcatgggcg tccgcgatcc gcttttccgt cattttggcg ccaagcagcg
     1801 agccgccgtc gccgtttacc gaatagccgg cgacgcgaaa gccgagtccg cgtatctgct
     1861 tgatcgccga gcggctatag cgcgcggtcg aaccccggaa ccagcgcggg tcggctcccc
     1921 ccgccgtgcg aagcgcggct gcgccgcctt cgacttccgc ggccaccgcc tggggcgatc
     1981 cggccgcggc gatgccgtaa atcttcatcg aacggtccac cgccggcaca tgcatggcgc
     2041 cgtggttctg gatttcgaac agttccggat gcgccttcaa ctgggcaaag gcatgctggt
     2101 tgtatttcag ccagcgcccg gtgacgaaga tcgtcgccgg cagcttgtta tcgaccagcg
     2161 ccgaaaggat gcgcgcatcg accttgccca tgcaggcatc gaaggtcagc gccacgcgcg
     2221 gaccggccgt tccgcccggc gcgacgtgaa gggtgggttc aacgatatcc gccggggtct
     2281 tggcgaacgc cgttccggca caggccagaa tggcggcgaa aacgcaaatg aacaggcgca
     2341 tgactactcc gggtatcgat cgggggccgc tttgaccccc gatcgaggcg aacttgcggc
     2401 attaccgcaa gtcgatgcca atctgattcc gacaatgctg gcagatgcct gttttaacta
     2461 gtctttttcg cctgcttcac gccttcttga ccgcggcctg cgccgccgcc agccttgcga
     2521 tcggcacacg gtagggcgag caggaaacat agtcgaggcc gacttcctcg cagaaatgga
     2581 cggaagccgg atcgccgcca tgctcgccgc aaattccgag cttgatttcc gggcgcaccg
     2641 accggccttt actggccgcc atgcgcatca gttcgccgac gccatcgacg tcgatcgaca
     2701 cgaacgggtc ctgctcgacg atgcctttct ggcgatatgt ctccaggaaa gaggcggcgt
     2761 cgtcgcgcga gatgccgaag gtggtctgcg tc
//
LOCUS       DASYCS010000392         5234 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1996067,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000392 DASYCS010000000
VERSION     DASYCS010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 5234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5234
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1996067"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..1516)
                     /locus_tag="VGM46_12170"
     CDS             complement(26..1516)
                     /locus_tag="VGM46_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4193393.1"
                     /translation="MRGISKTFGAVRANEAVDLQVAPGEILGLLGENGAGKTTLMNIL
                     FGAYAPDAGEILVNGKPASIHSSADALAAGIGMVHQHFHLASRLSVLENLLIGIPGRN
                     GRLDEAGAISRLDEISRRYGLKLDPHLAVSSLSIGEQQRLEIVKALFRGAKLLILDEP
                     TAVLAPTEVDGLFTALRSMASQGLGIIFISHKLNEVRALTHRCVVLRQGKVAGRVDTP
                     ANTTASEMAELMCGHEIVPPAKGPSRVGGTVLALERLSTARHSGMALRDVSLNLRSGE
                     ILGIAGVSGNGQRALADVISGMLKPASGTMTIAGVEVSAFTPREVQALGLGRIPEDRM
                     TTGLVTNLPLADSMVLPRIGTASFSRKGLLKPGAIRAFAQEQIEAYDIRCPGPMVRAG
                     ALSGGNLQKALLARELAFDPKVLIVAQPTRGLDIGAAHFIHEKFLDLRARGCGILVIG
                     EDLEELLTLSDRIAVMYEGRIVGTLDAADATVAKLGLLMTGAEAAA"
     gene            complement(1723..2745)
                     /locus_tag="VGM46_12175"
     CDS             complement(1723..2745)
                     /locus_tag="VGM46_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006201166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEY4193394.1"
                     /translation="MKNGNRNSLGGISRREVLSLGALGMAATVLPRGAFAQDKKLKVA
                     AIFATPIEEPWDNQVHVALQKAEKELGIEYKWSEKVQAADFGRVMREYAQGGYELVLG
                     DAFAAERESRRTAKQFPKTAFLFGSGAGPADPNFGVFDNWIHEPAYLSGLIAGKMSKS
                     GTVGAVAAMGIPEVNRLVNAFFAGARESHPGVKCKVSFIGSFFDPPKAKEAAIAQIDA
                     GVDVIYAERFGVIEAAAEKKVFAISNMSDQSSLAPDTVITGPVWDMYPTVEQAIKLVK
                     AGVFTAQDYGDFSRMAKGGSHLAPFHKFDATLPADVKELVEKKTAEIMEGNFRVDVDE
                     NTPTSD"
     gene            2946..3587
                     /locus_tag="VGM46_12180"
     CDS             2946..3587
                     /locus_tag="VGM46_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY4193395.1"
                     /translation="MARTTGSDGEKTEAAIREAAIDLMARYGYEAMSMRQLGAEVGVQ
                     AAALYRYFPTKEELLFTLMREHLEALMAAWRDARPAGGDAAARLGAFVENHIAFHMAR
                     RHSTHVSNMELRSLSPERLTAVLKLRTAYEKELRAILRDGAESGVFSVDDTGLTAMAL
                     IQMLTGVIVWFRPGERLSIAEVTATYLSMTMRLVGATMSGENQHVHARAELRA"
     gene            3556..4719
                     /locus_tag="VGM46_12185"
     CDS             3556..4719
                     /locus_tag="VGM46_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HEY4193396.1"
                     /translation="MYTHALNFGHDGDIDALRDTVRRFAGEQIAPLAAEIDRSNEFPA
                     HLWGELGALGLLGITADPDFGGTGMGYLAQTIAIEEISRASASVGLSYGAHSNLCVNQ
                     INRWATPEQKEKYLPDLCSGKTVGALAMSESGSGSDVVSLKLRAEKKNDRYVLNGTKM
                     WITNGPDAGTMVVYAKTDPERKARGITAFIVEKAFKGFSVAQKLDKLGMRGSNTGELV
                     FDNVEVPFENVLHQEGLGIEVLMSGLDYERTVLAGGPLGIMAACLDAAVPYVHERRQF
                     DRPIGEFQLVQGKLADMYTTLSASRAYVYAVAAACDRAETARKDAAGCILFAAEKATQ
                     MALDAIQLLGGNGYINDYATGRLLRDAKLYEIGAGTSEIRRWLIGREIMAEGV"
     gene            4719..>5234
                     /locus_tag="VGM46_12190"
     CDS             4719..>5234
                     /locus_tag="VGM46_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008839568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEY4193397.1"
                     /translation="MPVIQSQISPASDSFRDNAMHMRELVGDIADKAATVQRGGSEEA
                     RARHIGRGKLLPRERLAQLLDAGSPFLEVGQFAAWGLYGDEISSAGMIAGVGRIEGTE
                     VMVVVNDATVKGGTYYPLTVKKHLRAQEIALQNNLPCIYLVDSGGANLPNQDEVFPDR
                     DHFGRIFFNQAT"
BASE COUNT          968 a         1659 c         1670 g          937 t
ORIGIN      
        1 gcgctgcgcg cttcgaggcg aaacatcatg ccgcggcctc cgcgcccgtc atcaacaggc
       61 caagcttcgc cacggtggcg tcggccgcat ccagcgtgcc gacgatgcgg ccctcataca
      121 tgacggcgat gcggtcggag agcgtcagca actcctccag atcctcgccg ataaccagga
      181 tgccacaccc cctggcgcgc aaatccagga atttttcgtg gatgaagtgg gcagcaccta
      241 tatcgagacc gcgcgtcggc tgggcgacga tcagcacttt tgggtcgaaa gcgagttcgc
      301 gcgccagcaa ggctttttgc aaattaccgc cggaaagcgc gcctgcgcgg accatcggtc
      361 ccggacagcg aatgtcgtaa gcctcgatct gctcttgcgc gaaggcgcgg atcgcgccag
      421 gcttcagcag gcctttgcgg ctgaacgacg ccgtgccgat gcgcggcagc accatggaat
      481 cggccagcgg caggttggtg acgaggccgg tcgtcatgcg gtcttcgggg atgcggccaa
      541 ggccgagcgc ctgcacttcg cgcggcgtaa aggccgacac ttcgacgccg gcgatggtca
      601 tcgtgcctga tgccggcttc agcatgccgg aaatgacatc ggccagcgcg cgctggccgt
      661 tgcccgaaac gccggcgatg ccgaggattt cgcccgagcg caggttaagc gacacgtcgc
      721 gcagcgccat gcccgaatgg cgtgccgtcg atagtctttc gagcgccagg acggtgccgc
      781 cgaccctgga cggccccttt gccggcggca cgatttcatg gccgcacatc aactcggcca
      841 tttccgacgc ggtggtgttg gccggcgtat cgacgcggcc ggccactttg ccctgccgca
      901 acaccacgca gcggtgcgtc agcgccctca cctcgttcag cttgtgcgaa atgaagatga
      961 tgccaaggcc ctgcgaagcc atggagcgga gcgcggtaaa caggccatcg acctcggttg
     1021 gggcaagcac cgccgtcggc tcgtcgagga tcagcagctt tgcgccgcga aacagcgcct
     1081 tgacgatttc aagccgctgc tgctcgccga tggacagcga cgagacggca aggtgggggt
     1141 cgagcttcag cccataacgc cggctgatct cgtccaggcg cgaaatcgcg ccggcctcgt
     1201 ccagccggcc attgcggccg ggaatgccga tgagaagatt ttccagcacc gaaagccgcg
     1261 acgccaggtg gaaatgctgg tggaccatgc cgatgccggc ggccagcgca tcggcggaac
     1321 tgtggatcga ggcaggcttg ccattgacca ggatttcgcc cgcatcgggc gcgtaggcgc
     1381 caaacaggat gttcatcagc gtggtcttgc ccgcgccgtt ttcgccgagc aggcccagta
     1441 tctcgcccgg cgcgacctgc aaatccacgg cctcgttggc gcgcaccgcg ccgaacgtct
     1501 tggaaatgcc gcgcatttca atgaggggtg tggaggcggt caaggaaact ccggtcagaa
     1561 acgaaaatcc accctcccct tttccgggga ggatcggaga gagcggtttg cgcagcaaag
     1621 cgcccgaaac ggatttgggt gccggatact tcagcacctc acccaaagcc gcatgtcgct
     1681 tcgcgccctc cccataacgg ggagggtaaa cggtccgctc gctcagtcgg acgttggcgt
     1741 gttttcgtcc acgtcgaccc ggaagttgcc ttccatgatc tcggcggtct tcttctccac
     1801 cagttccttg acgtcggccg gcagcgtggc gtcgaacttg tggaagggcg ccaggtgcga
     1861 gccgcccttg gccatgcgcg agaaatcgcc gtaatcctgc gcggtgaaga cgcctgcctt
     1921 gaccagcttg atcgcctgct cgaccgtcgg atacatgtcc cagaccgggc cggtgatgac
     1981 ggtatcgggc gcgaggctcg actggtccga catgttggaa atggcgaaaa ccttcttctc
     2041 ggccgccgcc tcgatgacgc cgaagcgttc ggcgtagatc acatccacgc cggcgtcgat
     2101 ctgggcgatc gccgcctcct tggccttcgg cggatcgaag aacgagccga tgaaggacac
     2161 cttgcacttg acgccggggt gggattcgcg cgcgccggcg aagaaggcgt tgaccagccg
     2221 gttgacctcg ggaatgccca tggcggccac cgcgcccacg gtgcccgact tcgacatttt
     2281 tcccgcgatg aggcccgata aataagccgg ctcgtgaatc cagttatcga aaacgccgaa
     2341 attgggatcg gccggcccgg cgcctgagcc gaacaggaaa gcggtcttcg ggaactgctt
     2401 ggcggtgcgg cgcgactcgc gctcggccgc gaaagcgtcg cccagcacca gctcatagcc
     2461 gccttgcgca tattcgcgca tgacgcggcc gaaatcggcg gcctgcactt tttccgacca
     2521 cttatattcg atgccgagtt ccttctcggc cttctgcaac gccacatgga cctggttgtc
     2581 ccacggctct tcgatgggcg tggcgaaaat cgcggccacc ttcagcttct tgtcctgcgc
     2641 aaacgcgccg cgcggcagca cggtcgccgc cataccaagc gccccgaggc tcaacacttc
     2701 gcgccgggag atgccgccca gcgaattcct gttcccgttt ttcatcgaat gaccccttcg
     2761 gttgagacgg ttctgtgccg gctctgcctt gttgtaatct gcgcctgcaa actatggaac
     2821 cgcactttga ttttgtccac atggtcaaat ctgaagcacc atgcacaaaa tgccactgca
     2881 caaaggggaa cggcagcttg aattaattga acgttcgttt attactgtcc gcgaggagag
     2941 tttagatggc ccgcacgacc ggttccgacg gcgaaaagac cgaggccgcc attcgcgagg
     3001 cggcgataga cctcatggcg cgctacggct acgaggcaat gtcgatgcgc cagctgggcg
     3061 cggaggtcgg cgtacaggcc gccgccctct atcgctattt cccgaccaag gaagaattgc
     3121 tgttcacgct gatgcgcgag catctggaag cgctgatggc ggcgtggcgc gatgcccgcc
     3181 cggccggcgg cgatgcggcg gccaggcttg gcgccttcgt cgaaaaccac atcgcctttc
     3241 acatggccag gcggcactcg acgcatgtgt cgaacatgga actgcgcagc ctgtcgcctg
     3301 agcggctgac ggcggtcctc aagctgcgta ccgcctacga gaaggaactg cgtgccatct
     3361 tgcgcgacgg cgcggaaagc ggcgtctttt cggtcgatga caccggcctg accgcgatgg
     3421 cgctgatcca gatgctgacg ggcgtgatcg tctggttccg acctggcgag cggctgtcga
     3481 tagcggaagt gaccgcgacc tatctttcga tgacaatgcg cctggtgggt gccacaatga
     3541 gcggggagaa ccagcatgta cacgcacgcg ctgaacttcg ggcatgatgg cgacatcgac
     3601 gcgctgcgcg acacggttcg ccgcttcgcc ggcgagcaaa tcgcgccgct ggcggctgaa
     3661 atcgatcgtt cgaatgaatt tccagcacat ttgtggggcg aattgggcgc gcttggcctg
     3721 cttggcatca ccgccgaccc ggatttcggc ggcaccggca tgggctatct tgcgcagacg
     3781 atagccatcg aggaaatctc gcgcgcatcg gcctcggtcg gcctttccta tggcgcgcat
     3841 tccaatttgt gcgtcaacca gatcaaccgc tgggcgacgc cggagcagaa ggaaaagtac
     3901 ctgcccgacc tgtgttcggg caagacggtc ggcgcgctcg ccatgtcgga atccggttcc
     3961 ggctccgatg tcgtgtcgct gaaactgcgc gccgagaaaa agaacgaccg ctatgtgctg
     4021 aacggcacca agatgtggat caccaacggt ccggatgccg gcacgatggt ggtctacgcc
     4081 aagaccgatc cggaacggaa ggcgcgcggc atcaccgcct tcatcgtgga gaaggcgttc
     4141 aagggtttct ccgtcgcgca gaagctggac aagctcggca tgcgcggctc caacaccggc
     4201 gaactggtgt tcgacaatgt cgaggtgccg tttgaaaacg tgctgcatca ggaaggcctg
     4261 ggcatcgaag tgctgatgtc gggcctcgac tatgagcgca ccgtgctggc tggcgggccg
     4321 ctcggcatca tggccgcctg cctcgacgcg gccgtgcctt acgtgcatga acgcaggcag
     4381 ttcgaccggc ccatcggcga attccagctg gtgcaaggca agctggccga catgtacacc
     4441 acgctcagcg cttcgcgcgc ctatgtctac gcggtggccg ccgcctgcga ccgggcggag
     4501 acggcgcgca aggatgcggc aggctgcatc ctgttcgccg ccgaaaaggc gacgcagatg
     4561 gcgctcgacg ccatccagct gctgggcggc aacggctata tcaacgacta cgccaccggc
     4621 cgccttctgc gcgacgccaa gctctacgag atcggcgccg gaacgagcga aatccgccgc
     4681 tggctgatcg ggcgcgaaat catggcggag ggggtttaat gccggtcatc cagtctcaaa
     4741 tctcgcctgc ctccgacagc ttccgcgaca atgccatgca tatgcgcgag ttggtcggcg
     4801 atattgccga caaggcggcg accgttcagc ggggaggttc ggaagaagcg cgcgcgcgcc
     4861 acatcggccg gggcaagttg ttgccgcgtg agcggctggc gcagttgctg gatgccggtt
     4921 cgccctttct ggaggttggc cagttcgcgg cctggggcct ctatggcgac gagatttctt
     4981 ccgccggcat gatcgccggc gtcgggcgga tcgagggcac ggaggtgatg gtcgtcgtca
     5041 acgacgcaac cgtcaagggt ggcacctact atccgctgac cgtgaaaaag catctgcgcg
     5101 cgcaggagat cgcgttgcaa aacaacctgc cctgcatcta tctggtcgat tccggcggcg
     5161 cgaacctgcc caaccaggac gaggtttttc ccgaccgcga ccatttcggc cgcatcttct
     5221 tcaaccaggc cacc
//
LOCUS       DASYCS010000393         3702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2720715,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000393 DASYCS010000000
VERSION     DASYCS010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2720715"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            240..698
                     /locus_tag="VGM46_12195"
     CDS             240..698
                     /locus_tag="VGM46_12195"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006467341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEY4193398.1"
                     /translation="MNLAPSIALYQPDIAGNTGTILRLAACLGLDVHIIEPAGFDLSD
                     RNLKRAGMDYLEMAALTRHVDWNAFDEWRRLEARRLVLLSTRASLAYTRFTFVRGDIL
                     LFGRESAGVPDHVHDAADARLTIPMREGARSINLALSVAMVAGEATRQLG"
     gene            complement(831..1088)
                     /locus_tag="VGM46_12200"
     CDS             complement(831..1088)
                     /locus_tag="VGM46_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193399.1"
                     /translation="MRTPARPPLDGLKKRLDRSRAERAQGPRDGFLRETFVLSRSDAR
                     LKAKEWFERFPKQAYWTEIESWFERPGDVIEFTIRRLPAAD"
     gene            1202..2116
                     /gene="hemF"
                     /locus_tag="VGM46_12205"
     CDS             1202..2116
                     /gene="hemF"
                     /locus_tag="VGM46_12205"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HEY4193400.1"
                     /translation="MQRPEIPAGLPADIEAKKATAKGWFEELRDRICAGLEQIEDELA
                     GPNVSWTPGRFEKTPWRRDEGLGGGGTMSMMHGRVFEKVGVHTSTVHGEFSPEFRKQM
                     PGAEEDPRFWASGISLIAHPWNPNVPAVHMNTRMVVTSRQWFGGGADLTPVLDRRRTQ
                     DDPDTRAFHKVMRLTCDRNAGVADYERYKAWCDEYFHLAHRNEPRGTGGIFFDWLHSD
                     EEKGGWNADFNFVQEVGRSFLVVYPHLVRANFNENWTDGDREEQLIRRGRYVEFNLLY
                     DRGTIFGLKTGGNVAAILSSMPPLARWP"
     gene            2298..>3702
                     /locus_tag="VGM46_12210"
     CDS             2298..>3702
                     /locus_tag="VGM46_12210"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HEY4193401.1"
                     /translation="MLKRFLGDRSGNYAVIMSIMTLPLLLAAGFATDYVRYISAKQHL
                     QDMADAASLAAAASPERDDGKLKALASKMVVGNTAENRIDNVTVASWSNVDDKIDLGL
                     EGSIQTYFMGLANIRKLDVKASALAVRAVTGSVEVALVLDNTWSMSEADSSGKTKIDA
                     LKEAATSLTDKLIGDDGAENVRIGLVPYADYVNVGTQYRGSSWLSVPADSIGPTPPPQ
                     TCTMEDVSSTPCIGNAPSKTCTRYIDGVPENYECGKQCTSWGPTEIKTQKKCTGGGTA
                     TVKKWWGCVGSRMTGNTRLNDGSPSVPYPGYIETTQTCLNPIVPLTNNAASLDHSIDG
                     MIIKINNYRPYTYIPAGLIWGLNILSPLEPFNQAVAYDPQNKKPRKALVLMTDGENFM
                     NFRKSDGRHVAVPYPDYPEGFDPKNKQQADARAAQMKGTNDDTLAICNYIKEKGIEVY
                     TVAFMVDLDFSKELLQNC"
BASE COUNT          813 a         1157 c         1035 g          697 t
ORIGIN      
        1 ggaccagtgc gagcgtgctt gaaaattcag aaaaagcgtc ggaccggaac cgccggctga
       61 cacatcagcc aagcaagggg tggattggtg tagctggaca tcatcggttc ccccatcggt
      121 tcgagttgac aatggaccgc ctatagacgc attcgcgcaa aataaccaga tgccggccgc
      181 aaacgacggc agcttcaaac gccccgtggc cgacgcgcca cgctctgaaa ggcgaccaga
      241 tgaaccttgc gcccagcatc gcgctttacc agcccgacat tgccggcaac accggcacca
      301 tcctgcgcct ggccgcctgc cttgggctgg acgttcacat catcgaaccg gcgggcttcg
      361 atctgtccga ccgcaacctg aagcgcgccg gcatggacta tctcgaaatg gccgcgctga
      421 cccgccatgt cgattggaac gccttcgacg aatggcgacg gctggaagcg cggcggctgg
      481 tgctattgtc gaccagggcc agccttgcct acacgcgctt taccttcgta cgaggagaca
      541 tcctgttgtt tggccgcgaa tcggcaggcg ttccagacca tgttcatgac gctgccgacg
      601 cgcgcctgac cattcccatg cgcgaaggcg cgcgcagcat aaatcttgcc ctgtccgtgg
      661 cgatggtcgc cggcgaggcc accaggcagt tgggctaggc gctctcgcgc aggcggcaag
      721 caggacaatt gtgtttgatt gcccgcatga aaaaggtcgc cgcccccttc cgcccgattg
      781 gaatctattt tcaacggctt ggacgcgacg gctgaacctt ctccagcgct tcaatcggcc
      841 gccggcaagc gccgtatggt gaattcgatg acgtcgccgg ggcgttcgaa ccagctttcg
      901 atttcggtcc agtaggcctg cttgggaaag cgctcgaacc attccttggc cttcagccgc
      961 gcatcggaac gcgacaagac gaatgtttcg cgcaaaaatc cgtcgcgcgg cccttgcgca
     1021 cgctcggcgc ggctgcggtc cagccgcttc ttcagcccgt ccagcggcgg tcgtgcggga
     1081 gtgcgcatga aagaactcct tgacccttgc gcccaacaga ttacggatcg cgcccgcaaa
     1141 tgacgagtca tttgtcaggc ggccgacgat tcgcacaatg gccgccaccg gagacgacgc
     1201 cttgcaacga cccgaaatac cggccggcct gccggcagac atcgaagcca agaaagcgac
     1261 cgcgaagggc tggttcgagg aactgcgcga ccgcatttgc gccggcctcg aacagataga
     1321 ggacgagctt gccggcccga acgtctcatg gacgccgggc cgtttcgaaa aaacgccatg
     1381 gcggcgcgat gagggccttg gcggcggcgg caccatgtcg atgatgcatg gccgcgtgtt
     1441 cgaaaaggtc ggcgtccata cctcgaccgt ccacggcgag ttctcgcccg aattccgcaa
     1501 gcagatgccg ggcgccgagg aagacccgcg cttctgggca agcggcatct cgctgatcgc
     1561 ccacccatgg aacccgaatg tcccggccgt ccacatgaac acccgcatgg tggtgacatc
     1621 gcgccaatgg ttcggcggcg gcgccgacct caccccggtg ctggaccgcc ggcgcaccca
     1681 ggacgatccc gacacgcggg ccttccacaa ggtgatgcgc cttacctgcg accgcaacgc
     1741 cggcgtcgcc gactatgagc gctacaaggc ctggtgcgac gagtatttcc acctcgccca
     1801 ccgcaacgag ccgcgcggca ccggcggcat cttcttcgac tggctgcatt cggacgagga
     1861 aaaaggcggc tggaacgccg atttcaattt cgtccaggaa gtcggccgct cttttctcgt
     1921 cgtctacccg catctggtgc gcgccaattt caacgagaac tggaccgatg gcgaccgcga
     1981 ggaacaattg atccggcgtg gccgctatgt cgaattcaac ctgctttatg atcgcggcac
     2041 catttttggc ttgaagacag gcggcaacgt cgccgcgatc ctgtcttcca tgccgccgct
     2101 ggcacgctgg ccctagcagc cgggaaaatc cgtgcgcggc gcgaaatccc ctgcattttc
     2161 atgcacttct ttactactcg caaaccctct cgccactctt tgccgcttct catcgccgtc
     2221 gcttaccctt ttgcaaccgc ttgccgcgct tggtgaagcc ggcgcgcgtg agtttcgtgc
     2281 gatgggggtt tttaccgatg ctgaagcgtt ttctgggcga tcgctcgggc aactatgccg
     2341 ttataatgtc gatcatgacg ctgccgctcc tgctggcggc cggctttgcc accgactatg
     2401 tgcgctacat atcggccaag cagcaccttc aggacatggc cgacgccgcc tcgctggcag
     2461 ccgccgcctc gccggagcgg gacgacggca agctgaaggc gctggccagc aaaatggtcg
     2521 tcggcaacac ggccgaaaat cgtatcgaca acgtcaccgt ggcctcatgg agcaatgtcg
     2581 acgacaagat cgacctcggc ctcgaaggca gcatccagac ctatttcatg ggattggcca
     2641 acatccgcaa attggacgtc aaggcatccg cacttgccgt gcgcgccgta accggcagcg
     2701 tcgaagtggc gctggtgctg gataatacat ggtcgatgtc ggaagctgac tctagcggca
     2761 agacgaaaat cgatgccctc aaggaggccg ccaccagcct cacagacaag ctgataggcg
     2821 atgacggagc ggaaaatgtc cgtatcggac tggttcccta tgccgattat gtcaacgttg
     2881 gcacacaata tcgtgggtct tcatggctct ccgtcccggc tgattccata ggaccgactc
     2941 cgccacctca gacatgcacg atggaagacg tatcctcgac gccatgcata ggaaatgcgc
     3001 cctccaaaac ctgcaccagg tatatcgacg gcgttccgga aaattatgaa tgcggcaagc
     3061 aatgcaccag ttgggggccg acagaaatca aaacccagaa aaaatgcacc ggaggcggaa
     3121 cggctactgt caagaaatgg tggggatgcg tgggatcacg catgactgga aatacacgcc
     3181 tcaacgacgg cagcccctct gtcccttatc cgggttatat tgagacaacg caaacctgtc
     3241 ttaacccgat cgtacccttg accaacaatg ccgcatcact cgaccattcc atcgatggca
     3301 tgatcataaa aatcaataat tataggcctt atacctatat ccccgctggc cttatctggg
     3361 gtctcaacat cctttcgccg ttagaaccct tcaaccaagc ggtggcttac gacccccaaa
     3421 ataagaaacc gcgtaaagcc ttggtcctta tgacagacgg cgagaatttc atgaacttcc
     3481 gcaaatccga cggacgccac gtcgccgtcc cataccctga ttatccagaa ggcttcgatc
     3541 cgaaaaacaa gcaacaggca gatgccagag ctgctcaaat gaagggcaca aacgacgata
     3601 cattagcaat ttgcaattat atcaaggaaa aaggcatcga ggtctacacg gtagcgttca
     3661 tggtagatct agatttttct aaggaattac tgcaaaactg tg
//
LOCUS       DASYCS010000394         2325 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1561233,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000394 DASYCS010000000
VERSION     DASYCS010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1561233"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..814)
                     /locus_tag="VGM46_12215"
     CDS             complement(167..814)
                     /locus_tag="VGM46_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006333214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY4193402.1"
                     /translation="MKKTILAITAAAFLVSACTTDPYTGEQKVSNTVGGAGIGALAGA
                     ALGTLAGGNDRRNALIGAGIGALAGGAIGATMDQNEAELRRQLQGTGVSVTRTGDQII
                     LNMPSDITFASDEDSVKAGFYPTLNSVALVLKKFKQTTVDVFGHTDSTGGDQHNFDLS
                     QRRALSVANYLAGQGVDQRRFAVTGFGKTRPVASNATPEGRAQNRRVEIQLSPLT"
     gene            complement(926..2057)
                     /gene="prfB"
                     /locus_tag="VGM46_12220"
     CDS             complement(join(926..1990,1992..2057))
                     /gene="prfB"
                     /locus_tag="VGM46_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531367.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEY4193403.1"
                     /translation="MRAETQNIVDEIRQAITLLRRHFDWDQAIKRLDYLNVLAEDSSL
                     WNDPLEAQKLMRERQGLEDGITAVTRFTQALEDNISLIGMGEEEGDAGIIAEAEASLR
                     SMQAEAKARQVETLLSGEADGNDTYLEVHAGAGGTESQDWASMLLRMYTRWAERRRFK
                     VEVLEVHDGEEAGIKSATILIRGHNAYGWLKTESGVHRLVRISPYDSNARRHTSFSSI
                     WVYPVVDDKIEIDVSESDVRIDTYRASGSGGQHINTTDSAVRITHIATGIAVACQAER
                     SQHKNKAKAWEMLRSRLYEEELKKREAAANATEASKSDIGWGHQIRSYVLQPYQLVKD
                     LRTGIESTSPSGVLDGDLDDFMEASLSQRIEGGAGEAVADLD"
BASE COUNT          420 a          735 c          711 g          459 t
ORIGIN      
        1 caaaatgcag gatcgcgtcc gtcaaactca accgcactac ggatgaccga ctacttggcg
       61 tcgggacatc gactggaaaa cggccgtcaa aaaaaaacgg ccccttgcag ggccgtttaa
      121 cgttcggcac aagccgtaaa atggttggtg cgagaacgca gcccgctcag gtaagcggtg
      181 aaagctggat ttcgacgcgg cgattctgcg cgcgaccttc cggggtggcg ttggaagcga
      241 caggccgtgt cttgccgaaa ccggtaaccg caaagcgacg ctggtcgacg ccctgcccgg
      301 ccaggtaatt ggcgaccgaa agggcgcggc gctgcgacag gtcgaaatta tgctggtcgc
      361 caccggtcga atcggtgtgg ccaaacacat ccaccgtggt ctgcttgaac ttcttcagca
      421 ccagcgcgac cgaattcagc gtcgggtaga agccggcctt caccgaatcc tcgtcgcttg
      481 caaaggtgat gtcggacggc atgttgagga tgatctggtc gccggtgcgc gtgacgctga
      541 cgccggtgcc ctgaagctgg cggcgcagtt ccgcctcgtt ctggtccatc gtcgcgccga
      601 ttgcgccgcc cgccagcgcg ccgatgcccg cgccgatcaa tgcgttgcga cggtcattgc
      661 cgccggcaag cgtgccaagc gcggcaccgg ccagcgcgcc gatgccggcc ccgccaaccg
      721 tgttggaaac cttctgctca ccggtatacg gatcggtcgt gcaggcgctc acaaggaaag
      781 ctgccgccgt aatggcgagt atggttttct tcatggataa atccctctca atgcggtcgc
      841 cagtggcgcg ccggtcaccc ttcacggcct tgtagcataa aatgaggcta aatatggaat
      901 ggaaagctgg aggccgaccc gctccttaat ccagatcggc aaccgcctcg ccggccccgc
      961 cctcgatgcg ctgcgacagc gacgcctcca tgaaatcgtc gaggtcgcca tccagcacgc
     1021 cggacgggct ggtgctttcg atgccggtgc gcaaatcctt gaccagctgg tagggttgca
     1081 gcacgtagga ccgaatctga tggccccagc cgatgtcgct tttcgaagcc tcggtcgcat
     1141 tggctgccgc ctcgcgcttc ttcagctctt cctcgtaaag gcgcgagcgc agcatttccc
     1201 aggccttggc cttgttcttg tgctgagaac gctcggcctg gcaggccacg gcgatgccgg
     1261 tggcaatatg cgtgatgcgc acagccgaat cggtggtgtt gatgtgctgg ccgcccgaac
     1321 ccgaagcgcg ataggtgtcg atgcgcacat ccgattcgga aacgtcgatc tcgatcttgt
     1381 cgtcgacgac cggatagacc cagatgctgg aaaaggatgt atgccgacgc gcgttcgaat
     1441 cgtagggcga aatgcgcaca agccgatgaa cgcccgattc cgtcttcagc cagccatagg
     1501 cgttgtggcc gcgaatgagg atcgtggcgg acttgatgcc ggcttcttcg ccgtcatgca
     1561 cctccagcac ctcgaccttg aagcggcggc gttccgccca gcgcgtatac atgcgcaaaa
     1621 gcatcgacgc ccaatcctgg ctttcggtgc cgccggcgcc cgcatggact tcgagatagg
     1681 tgtcgttgcc atcggcttca ccggagagca gcgtttccac ctgtcgcgcc ttggcttccg
     1741 cctgcatcga gcgaagcgag gcttccgcct cggcgatgat gccggcgtcg ccctcttcct
     1801 cgcccatgcc gatcaggctg atattgtctt ccagcgcctg cgtgaaccgg gtgacggcgg
     1861 tgatgccgtc ctcaaggccc tgccgctccc gcatcagctt ctgcgcttcc agcggatcgt
     1921 tccagaggct tgaatcttcg gccagcacgt tcaggtaatc aagccgcttt atggcctgat
     1981 cccagtcaaa gatgcctcct cagcagggtt atcgcctgcc tgatttcgtc aacgatgttc
     2041 tgcgtttccg cgcgcatggc cggtcttctg atcctgaggt tggatggggt tggcgcgata
     2101 catagggatg cgcctcccgc ctgtaaagcg aaatggcggc cgtcagggcc gccatcgcca
     2161 tgctgtggaa ctagtagagg ccaccgccgc cggactggat cgcctgcttg gcgcttggcg
     2221 aaatatcgcc gccggtgccg ttggagccgt catcgcccat gccgatcacc caatagctgt
     2281 cggcggggcc ggtgcccggc ttgaacgcct cgatgatcgt acccg
//
LOCUS       DASYCS010000395         6762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_255969,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000395 DASYCS010000000
VERSION     DASYCS010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 6762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6762
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255969"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VGM46_12225"
     CDS             <1..462
                     /locus_tag="VGM46_12225"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY4193404.1"
                     /translation="PTETLASYTPPKKEKVIQQAEDDNAAAPDSTGIGKMRWPVRGRV
                     ISSFGGGKDGDGIDISVPEGTPVKAAENGVVIYAGNGLKEFGNTVLVRHEDGLVTVYG
                     HASSLEVTRGQKVKRGQEIALSGISGTTDSPKLHFEVRKNSAPVDPKGFLE"
     gene            complement(649..1518)
                     /locus_tag="VGM46_12230"
     CDS             complement(649..1518)
                     /locus_tag="VGM46_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019170466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY4193405.1"
                     /translation="MTDNALDALNRKLDSLIEAVSRLAPVPAPQTDLAGADCFVWQAD
                     RGYLEPVQKVNRVDIGLIRGVDRVRDILVDNTERFAKGLAANNVLLWGARGMGKSSLV
                     KAVHAQINAAGGNPLKLIEIHREDIDTLPRLMTLLKAAPYRFILFCDDLSFDHDDTSY
                     KSLKAALEGGVEGRPSNVIFYATSNRRHLLPRDMIDNERSTAINPGEAIEEKVSLSDR
                     FGLWLGFHKCSQDDYLAMVDGYARHYGLETDAETLRAEALEWATTRGSRSGRVAWQFS
                     QDLAGRLGKPLND"
     gene            1693..2034
                     /gene="yajC"
                     /locus_tag="VGM46_12235"
     CDS             1693..2034
                     /gene="yajC"
                     /locus_tag="VGM46_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEY4193406.1"
                     /translation="MFVTPAYAQGIGGASPDMLISILPFVLIFVIMYFLIIRPQRTQL
                     KKRGEMLAAVRRGDTVVTGGGLIGKVTKVIDDNELEIDLGNANKVTALRSTIAEVRVK
                     GEPVANQNAKK"
     gene            2084..4630
                     /gene="secDF"
                     /locus_tag="VGM46_12240"
     CDS             2084..4630
                     /gene="secDF"
                     /locus_tag="VGM46_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HEY4193407.1"
                     /translation="MLYFSRWKTIGIWAIVLLGMFFAAPNLFPQSVLGKLPDWLPKKQ
                     MTLGLDLQGGSHILLKIDPNDLIKERLETARDEIRTSLRDAKVGYTGLSGTGKSVQVR
                     IRDAAQVDAAKTALKALTDPVASGLLAGGSITEMELTEPEPGVLRYTITDAGLSYRMS
                     SAVAQSIEVIGRRVNELGTTEPIVQRQGSDRILVQVPGLQDPQRLKDILGQTAKLTFQ
                     MVDQSVPVQDALSGRAPAGSTIMYSKDQPPVPYLIENRVIVSGESLVDAQATFDQRTS
                     EAVVSFRFDSKGATRFGQATQQNVGKLFAIILDGHVISAPQIREPILGGTGQISGNFT
                     AQSANDLAVLLRAGALPATLTIIEERTVGPGLGQDSIQAGKMSALVASILVVTFMVAA
                     YGTLGWIANLALIANLALIVAVLSLVGSTLTLPGVAGIVLTVGMAVDSNVLIYERIRE
                     ERRAGRSVLQSVDIGFQRALATILDSNITTLIAAIVLFFLGSGPVRGFAVTLAVGIIT
                     TVFTAFTLTRWMAAEWLRRARPKELPKGYVRLVPEVTHIRFMRIRLQVFVLSIALCVA
                     SVGLLLTHGLNFGIDFTGGTLMEVRAKTGDADVGDIRSRLSKAGIDEIQVQSFGTQTD
                     ALIRIGAMADTGDESGNAIVKKAQDSLTSSYDIRRTELVGPTVSSELAWAAILGVVVA
                     MGAILIYVWIRFEWQFAVGSIIATINDITLTLGFISITGLQFDLTTVAAILTIVGYSL
                     NDTVVIYDRVRENLRRFKRMPLGQLLDLSDNEMLSRTTMTSITTFLALLALFVLGGEV
                     IRSFVASMLFGVFIGTFSSIFIAAPILILFKLRPSSATSNEGAAQQLPAS"
     gene            4630..5019
                     /locus_tag="VGM46_12245"
     CDS             4630..5019
                     /locus_tag="VGM46_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010661252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HEY4193408.1"
                     /translation="MGAGIVIREAFFPGRAPIEAYGNGGFRFADMSHRGSLLCLPSGI
                     YGWEPADPLALTQADFEKLFAQAGQVEILLVGCGTDLRRLPAELRIALKDAGIASDAM
                     STGAAVRTYNVLLAEERAVAAALIAVD"
     gene            5024..5872
                     /locus_tag="VGM46_12250"
     CDS             5024..5872
                     /locus_tag="VGM46_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HEY4193409.1"
                     /translation="MDDTAKIVMDTVRAGDRDRYLSALYAPAEKRDGLLALYAFNTEI
                     AGVRDRIHEALPGEVRLQWWRDIIAAGGDANAAGHPLATALKNTIAAFRLPGQAFDNY
                     LEARIFDLYDDPMPSRTDLEGYCGETASTLIQLAAMVLDDGAAPGIAELAGRAGCAQA
                     MTGLLMLLPLHRSRGQCFVPVDILNAVGSTPEEFLAGDGGPAATRAIAAMIALAREHL
                     AAFLAGAPSLPPSLRPAFLPLALSQAYLDRMQRPDTSPLRRTVGLSGLRRHWLLFRHA
                     SRGWSA"
     gene            complement(5914..>6762)
                     /gene="trmFO"
                     /locus_tag="VGM46_12255"
     CDS             complement(5914..>6762)
                     /gene="trmFO"
                     /locus_tag="VGM46_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009451733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEY4193410.1"
                     /translation="KVGPGGTGKDYINCPMDKDQYGAFVQALIDGEKTEFKQWEGTPY
                     FDGCLPIEVMAERGAETLRHGPMKPMGLTNAHNPEVKAYAVVQLRQDNALGTLYNMVG
                     FQTKLKHAEQVRVFRTIPGLENAEFARLGGLHRNTYINSPTLLDGSLQLKSRPGLRFA
                     GQITGCEGYVESAAIGLLAGRFAAAQRLGAQLAPPPITTAFGALLNHITGGHIVSEDE
                     PGKRSFQPMNINFGLFPPMDAPKIDGKRLRGKEKTVAKKKAMTARALVDFRQWLDGAG
                     YAVAAE"
BASE COUNT         1172 a         2174 c         2116 g         1300 t
ORIGIN      
        1 ccgaccgaga cgctggcttc ctatacgccg cccaagaagg aaaaggtcat ccagcaggcg
       61 gaagacgata atgccgccgc acccgattcg accggcatcg gcaagatgcg ctggccggtt
      121 cgcggccgcg tcatttccag cttcggcggc ggcaaggacg gcgatggcat cgacatttcc
      181 gtgccggaag gcacacccgt caaggctgcc gagaacggcg tcgtcatcta cgccggcaat
      241 ggcctcaagg agttcggcaa caccgtgctt gtgcgccatg aggatgggct ggtgacggtc
      301 tacggccacg ccagttcgct ggaagtcacg cgcggccaga aggtcaagcg cggccaggaa
      361 atcgcgctgt ccggcatcag cggcaccacc gattcgccaa aactgcattt cgaggtgcgc
      421 aagaattccg cgccggtcga tccgaagggt ttcctggaat aagcgcaatc agcgacttgc
      481 gggccgcctg acctccagtc cggcccgccg gcttggcccg tcccgaaaaa gggggcgggc
      541 cttgtcagtt cagagcattt ccagcaaaag tgtgaaacgg ttttgcatcc ggaaatgcgg
      601 ccaaagcgac gggatggacc ggtctttccg aatcggtgaa agccaagtct agtcgttgag
      661 cggcttgccc agcctgccgg cgagatcttg gctgaactgc caggcgacgc ggcccgaacg
      721 gcttccgcgc gtcgttgccc attccagcgc ctcggcgcgc aacgtttcgg cgtccgtctc
      781 caggccgtaa tggcgggcat agccgtccac catcgccaga taatcgtcct gcgagcattt
      841 gtggaagccg agccacaggc cgaaacggtc cgacagcgaa accttttcct cgatggcctc
      901 gcccggattg atggcggtcg aacgctcatt gtcgatcatg tcgcgcggca gcaggtgccg
      961 gcgattggag gtggcgtaga agataacatt gctcggccgc ccctcgacgc cgccttcaag
     1021 cgccgccttc agcgacttgt aggaggtgtc gtcgtggtcg aaagacagat cgtcgcaaaa
     1081 caggatgaag cgatagggcg cggccttcaa aagcgtcatc agccggggca gggtgtcgat
     1141 gtcctcgcgg tggatttcga tcagcttgag cggattgccg ccagccgcat tgatctgggc
     1201 atgaaccgcc ttgaccagcg aagacttgcc catgccgcgc gcgccccaca aaagcacgtt
     1261 gttggcggca agcccttttg cgaagcgctc ggtattgtcg accaatatgt cgcggacacg
     1321 gtcgacgccc cggataaggc cgatgtcgac gcggttgacc ttttgcaccg gctccagata
     1381 gccacggtcg gcctgccaca cgaaacaatc ggccccggcg aggtcggttt gcggcgcagg
     1441 cacaggcgcc aggcgtgaga cggcctcgat caggctgtcc agtttccggt tcagggcgtc
     1501 gagggcatta tcggtcatag cgtcactttt atgtttgcat tggcggcgaa tgacgctcta
     1561 acacgggcca acaggccgga aaagcagcgg atttgcctgt ccacgcagtt gcaatggtaa
     1621 ctttaccgac tatattccgg ccaatttcgc ggaccgcctc gcggccgctc ttcccaattc
     1681 aggagctatt cgatgtttgt tactccggca tacgcccaag gcatcggcgg cgcttctccc
     1741 gacatgctca tcagcatcct gccgttcgtg ctgattttcg tcatcatgta tttcctcatc
     1801 atccgcccgc agcgcaccca gctgaagaag cgcggcgaga tgctggcggc cgtgcggcgc
     1861 ggcgacaccg tcgtcaccgg tggcggcctc atcggcaagg tgaccaaggt gatcgacgac
     1921 aacgaactgg aaatcgacct tggcaatgcc aacaaggtga ccgcgttgcg ctccaccatc
     1981 gccgaggttc gcgtcaaggg cgagccggta gcgaaccaga acgccaagaa ataaccgtat
     2041 tgaaggcgga ccgccgcttg tacgcctgaa cggatagtcg cttatgctgt atttttcgcg
     2101 ttggaagacg attggcatct gggcaattgt gctgctcggc atgttttttg ccgcacccaa
     2161 tctttttccg caatcggttc tcggcaagct gccggactgg cttcccaaga agcagatgac
     2221 gcttggcctc gatcttcagg gcgggtccca tatcctgctc aagatcgatc cgaacgacct
     2281 gatcaaggaa cggctggaaa ccgcgcgcga cgaaatacgc accagcctgc gcgacgccaa
     2341 ggtcggctat accggccttt ccggcaccgg caagtcggtg caggtgcgca tccgcgacgc
     2401 cgcccaggtc gatgcggcca agacggcgct gaaggcgctg acggaccccg tcgcctccgg
     2461 gcttctggcc ggtggctcca tcaccgaaat ggagctgacc gagcctgagc cgggcgtgct
     2521 gcgctacacg attaccgatg ccggcctgag ctaccgcatg tcgagcgcgg tggcgcaatc
     2581 gatcgaggtg atcggccgcc gcgtcaacga acttggcacg acagagccga tcgtgcagcg
     2641 ccaggggtcg gaccgtatcc tggttcaggt gccgggcctg caagacccgc agcggctgaa
     2701 ggacattctc ggccagaccg ccaagctgac cttccagatg gtcgatcaat ccgtgcccgt
     2761 gcaggacgcg ctcagcggcc gcgcgccggc cggctccacc atcatgtatt cgaaagacca
     2821 gccgccggtt ccgtacctga tcgagaaccg cgtcatcgtt tcgggtgaaa gcctcgtcga
     2881 tgcgcaggcg actttcgacc agcgcacgag cgaagcagtc gtctcgttcc gcttcgattc
     2941 gaagggggcg acgcgtttcg gccaggcgac gcagcagaat gtcggcaagc tgttcgccat
     3001 catcctcgac ggccatgtga tttccgcccc gcagatccgc gagcctatcc tcggcggcac
     3061 cgggcagatt tccggcaact tcacggccca gagcgccaac gaccttgccg ttctgctgcg
     3121 cgccggcgcg ctgccagcga cgctgacgat aatcgaggaa agaacggtcg gtccgggcct
     3181 cggccaggat tcgatccagg caggcaagat gtcggcactc gtcgcttcga tcctcgtcgt
     3241 gacgttcatg gtcgccgcct atggcaccct tggctggatc gccaatctcg cgctcatcgc
     3301 caatctggca ctcatcgtcg ccgtgctctc gcttgtcggc tcgacgctga ccttgcccgg
     3361 cgtcgccggc atcgtgctga ctgtcggcat ggcggtcgat tccaacgtgc ttatctatga
     3421 gcgcatccgc gaggagcgac gagccgggcg ctcggtgctg caatcggtcg atatcggctt
     3481 ccagcgcgcg ctggcgacga ttctggactc caacatcacc acgcttatcg ccgccatcgt
     3541 gctgttcttc ctggggtccg gcccggtgcg cggctttgcc gtcacgcttg ccgtcggcat
     3601 catcaccacc gttttcaccg ccttcacgct gacgcgctgg atggcggcgg agtggctgcg
     3661 ccgcgctcgc cccaaggaat tgccgaaggg ctatgtccgc ctcgtgccgg aagtcacgca
     3721 tatccgcttc atgcgcattc gcttgcaggt gtttgtcctg tcgatcgccc tttgcgtcgc
     3781 ctcggtcggc ctgctgctga cccatgggct gaacttcggc atcgacttca ccggcggcac
     3841 gctgatggaa gtgcgggcca agaccggcga tgccgatgtc ggcgatatcc gctcgcggct
     3901 gtcgaaggcc ggtatcgacg agattcaggt gcagagcttc ggcacgcaga ccgatgcgct
     3961 catccgcatc ggcgccatgg ccgacaccgg cgacgaatcc ggcaacgcca tcgtcaagaa
     4021 ggcgcaggat tcgctgactt caagctacga tatcaggcgg accgaactgg tcggcccgac
     4081 cgtttccagc gaacttgcct gggccgccat actcggcgtg gtggtcgcca tgggggcgat
     4141 cctgatctat gtctggatac ggttcgagtg gcagttcgcc gtcgggtcga tcatcgccac
     4201 gatcaacgat atcacgctga cactggggtt catttcgatc accgggttgc agttcgatct
     4261 caccacggta gccgccatcc tgaccatcgt cggctattcg ctcaacgata cggtcgtcat
     4321 ctacgaccgg gtgcgtgaga atttgcggcg cttcaagcgc atgccgctcg gccagctgct
     4381 cgatctgtcg gacaatgaaa tgctgtcgcg cacgacgatg acgtcgatca ccacgttcct
     4441 tgccctgctg gctctgttcg tgctgggcgg cgaggtcatc cggtcgttcg tcgcctcgat
     4501 gctgtttggc gtgttcatcg gcaccttctc gtcgatcttc atcgccgcgc cgatcctgat
     4561 cctgttcaag ttgcggccgt catcggcaac ctcaaacgag ggtgcggcgc agcaattgcc
     4621 cgccagctga tgggggcggg catcgtcatc cgcgaagcgt tctttcccgg acgcgcgccg
     4681 atagaagcct acggcaatgg cggtttccgc ttcgccgaca tgtcgcatcg cgggtcgctt
     4741 ttgtgcctac cttccggcat ttacggctgg gagcctgccg atccgctggc attgacgcag
     4801 gctgatttcg aaaagctgtt tgcgcaggcc gggcaggtgg aaatcctgct ggtcggctgc
     4861 ggcaccgatt tgcggcggct gccggcggaa cttcgaatag cgctcaagga tgccggcatc
     4921 gccagcgatg ccatgtccac cggcgctgcg gtgcgaacat acaacgtgct gctggccgaa
     4981 gagcgcgccg tggcggcggc gctgatcgcc gtcgactgaa gcgatggacg atacggccaa
     5041 gatcgtcatg gacacggtgc gcgccggcga ccgcgaccgc tatctctcag cgctctatgc
     5101 gcccgccgaa aagcgcgatg ggctgctggc gctttatgcc ttcaacaccg aaatcgccgg
     5161 cgtgcgcgac cgtatccacg aggcgctgcc cggtgaagtt cgcctgcaat ggtggcgcga
     5221 catcatcgct gccggcggcg acgccaatgc ggcggggcat ccgctggcga ccgcgctgaa
     5281 aaacaccatc gccgcctttc gtctgccggg gcaggctttc gacaattatc tcgaagcgcg
     5341 catcttcgac ctctacgacg atccgatgcc gtcgcgcacc gatctcgaag gctattgcgg
     5401 cgagacggcc tcgacgttga tccagctcgc cgcgatggtg ctggacgatg gtgcggcacc
     5461 cggcatcgcc gaactggcgg gccgcgccgg ctgcgcgcag gcgatgaccg gcctgctcat
     5521 gctgctgcca ttgcataggt cgcgcggcca gtgcttcgtg ccggttgata tcctgaacgc
     5581 ggtaggctcg acgccggaag agtttctggc aggcgatggc gggccggcgg cgacgcgcgc
     5641 aatcgcggcc atgatcgcgc tggcgcggga gcatctggcg gctttcctgg ctggtgcacc
     5701 cagcctcccg ccatcgctgc gccccgcatt cctgccgttg gccttgtccc aggcctatct
     5761 cgacaggatg caaaggccgg atacctcgcc tttgcgccgg acggtcggcc tgtcggggct
     5821 aaggcgccac tggctgctgt ttcggcacgc gtcgcgcgga tggagcgcct aggcaactcc
     5881 aaggcaagcg ggcagcgttt ttcccgtcag ggtctattcg gcggcgaccg catagccggc
     5941 gccgtccagc cattgccgga aatcgaccag cgcgcgcgcc gtcatcgcct tcttcttggc
     6001 gacggtcttt tccttgccgc gcaggcgctt gccgtcgatt tttggcgcgt ccatcggcgg
     6061 aaacaggccg aaattgatgt tcatcggctg gaacgagcgt ttgcccggct catcctccga
     6121 aacgatatgg ccaccggtga tgtgattgag cagcgccccg aaggccgtgg ttatcggcgg
     6181 cggggcaagc tgcgcgccaa gccgctgcgc tgccgcgaag cgcccggcga gcaggccgat
     6241 tgcggcgctt tcgacatagc cctcgcagcc ggtgatctgg ccggcaaagc ggaggcctgg
     6301 ccgcgacttc agttgcagag agccgtcgag cagcgtcggc gaattgatgt aggtattgcg
     6361 gtgcaggccg cccagccgcg cgaattcggc gttttcgagg cccggaatgg tgcggaagac
     6421 gcgcacctgc tcggcgtgct tcagcttggt ctggaacccg accatgttgt agagcgtgcc
     6481 cagcgcatta tcctgccgaa gctgcaccac cgcataggcc ttcacttccg gattatgcgc
     6541 gttggtcagg cccatcggct tcatcggccc gtgccgcagc gtctccgcgc cgcgctcggc
     6601 catcacctcg atcggcaggc agccgtcgaa atagggcgtg ccttcccatt gcttgaattc
     6661 ggtcttctcg ccatcgatca acgcctgcac gaaggcgcca tactggtcct tgtccatcgg
     6721 gcagttgata tagtccttgc cggtgccgcc cggcccgacc tt
//
LOCUS       DASYCS010000396        11898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3880304,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000396 DASYCS010000000
VERSION     DASYCS010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 11898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11898
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3880304"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="VGM46_12260"
     CDS             <1..454
                     /locus_tag="VGM46_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEY4193411.1"
                     /translation="GQAQGLGDRLGVLLVQLPPSLRFAPEVAEGFFAMLRGKTMVGIA
                     CEPRHASWFGTEADALLASHQIARVAADPARHEGAGQPGGWPGLAYFRLHGVPRIYYS
                     DYGDERLREIRQRMAACHEAGAKVWCIFDNTAEGHALGNALTLDGAAP"
     gene            complement(464..1633)
                     /locus_tag="VGM46_12265"
     CDS             complement(464..1633)
                     /locus_tag="VGM46_12265"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY4193412.1"
                     /translation="MAQYDIVIIGGAIVGSSLAYYLRKEGFSGSIALVERDPQFTHSA
                     TTLSAASIRQQFSIPQNIRLSQFTLGLFKRLKEEFGEDADIGFREGGYLILAGENGLP
                     VLKANHAVQVAEGADIVLEDAATLSRRFAWLSTEDIAAGAYGVSGEGWFDAHAYLQLF
                     RKALRSKNIDLITATVTGISSSGARVTGVTLDNGETLDAGIVVNAAGPNGGKVAALAG
                     LALPVEPRKRTVFVFEARDKFADMPLLVDPSGIYVRPEGSVYIAGGAEVEEGEQAADP
                     ADFEPVWSHFEETIWPVLATRIPAFEAIKMTRAWAGHYDYNALDQNAVIGPHPDVSNF
                     IFANGFSGHGLQQAPAVGKSLAEFIVHGAYRTVDCSAFGYQRIAERRPFRELNVI"
     gene            1732..2613
                     /locus_tag="VGM46_12270"
     CDS             1732..2613
                     /locus_tag="VGM46_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEY4193413.1"
                     /translation="MTTDGFRPRRSVLYVPAANDRALAKMATLGCDAVIIDLEDSIAP
                     ADKILAREKLKGIFASRPRRRCEMVVRINGLATEWGADDLLAAAACEPDGILLPKVDT
                     PRDVLEAGDALDDAFAPDEVKLWAMIETPKAMLNIGAIAELGRDPASRLNCFVAGTND
                     LVKETGILATPDRRYLLPWLMQMVLAARAAGLDLLDGVFNDFGDADGFARECTEAANM
                     GFDGKTLIHPAQVEPANSAFAPSAQAVAQAKAIHDAFALAGNANKGVISLDGKMVERL
                     HLAQAEKLLAKAAAIGA"
     gene            2645..2854
                     /locus_tag="VGM46_12275"
     CDS             2645..2854
                     /locus_tag="VGM46_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006326895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HEY4193414.1"
                     /translation="MRLYRFLSAIDDASFCHKVTAALNKGWHLYGSPTYAYDKKEKIM
                     RCGQAVVKDIEGQEYEAGKTKLSDW"
     gene            complement(2873..3295)
                     /locus_tag="VGM46_12280"
     CDS             complement(2873..3295)
                     /locus_tag="VGM46_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEY4193415.1"
                     /translation="MARIYKTRTWHDELSPSLEEIEFLAMETYAHLPEEFRKLTGEIV
                     IQVAEFPTDEIMDDLSLETPFDLLGLFEGRGIAERWNPQTGDGPNRITLYRRAMLDYW
                     TENEETLGDIVTHVLIHEIGHHFGLSDDDMEKLEEEAQ"
     gene            3488..4546
                     /locus_tag="VGM46_12285"
     CDS             3488..4546
                     /locus_tag="VGM46_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193416.1"
                     /translation="MAKDAVAREIVFALRRQIAKIEGTLPECLEAPARADITVVRRGG
                     QAEGNDALSNRALASLALSTGAGCLDAALGGGLPQGALTEIHGLETRDAGAVTGFVLA
                     LAGLAVGAAQGEAGLATPIVWIGMTETFGEAGFPYARGLQSSFGVGPESLLFCEARKL
                     ADALWIAEEAARLSTSAAVVLEIRGNPRRLDLTATRRLHARAQQTGRPLFLLRQAALA
                     EPTAAPVRLVVLPAPSGLRETVAGPLTGSIGLPAFAVSISKSAYASNGQFILEWNPDE
                     RIFMERGAGERSAGEIRAKDPFAVVSLPRHGADSAAAPGAVVAFKPALGRQARKPAAG
                     DQPPRPQRAAHRGARRAG"
     gene            4488..6002
                     /locus_tag="VGM46_12290"
     CDS             4488..6002
                     /locus_tag="VGM46_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HEY4193417.1"
                     /translation="MISHRDRNAQRIAALDERAEALGLKRGMGIADARAMHPAIDIEE
                     ADAQADRLLLEGLADWCDRYTPLVALDGADGLFLDIAGCAHLFGGERAMLDTMLTRFL
                     QQGFIARAGIASAAGAAWAAARFASGSIVAVGEEAALLAPLPLSALRIEPAVRESLES
                     VGLRRVGAVMATPRAPLARRFGALLLERLDQALGRSDEAVSPRLPAAPLSVERHLAEP
                     ITQIEEIERLTASLSQTLKHDLERRGEGARALGLMLFRVDGAVSQIAIGTSRPLRDPL
                     LIQKLFHERLAATGQEYEVGYGFDLVRLSVLSVAAFDMQQTDLTGEIKDAEADIALFA
                     DRVRARLGDNAVRRPISVASHLPERSIALVAFTDAPASKSKNRVPSISLSERPVRLFR
                     SPEPVEVPATELPEGPPLHFRWRRALYRVVRAEGPERIAPEWWRESIPDAMAKEGEDA
                     GEVKNRAVAEKTASLTRDYFRIEDAEGRRYWLFRQGLYDAGRTSPRWFMHGIFA"
     gene            5999..9364
                     /locus_tag="VGM46_12295"
     CDS             5999..9364
                     /locus_tag="VGM46_12295"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018430420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HEY4193418.1"
                     /translation="MKPEPPCDTPGASRYIEFGVQSNFSFLRGASRPEELVVAASLLG
                     YAGIGLADRNSVAGVVRAWSQSKHIKPAADAAPAAFAYRPGCRLVFCDGMPDVLAYPR
                     DREGWGHLCRMLTQANLRDENEKGATLLRGADLLEWGERMSLAVLADLSRAKDQMTLL
                     RQIKDRFGEAVRLAVAPAYRGDDRFRIEQAAAMASALGLRLMATNDVLYHTSARRPLQ
                     DVLTAIRLNVPVAEAGLELAANAERHLKPPAEMARLFRRYPEAVEETWRFKKELGFSL
                     GELQHNYPDEPTRDGATPQQELERLAKEGAAERYPGGAPASVTERIREELRLIEKMGY
                     ARYFLTVHDIVRFARSQGILCQGRGSAANSVVCFCIGITPVGPDKIDTLFERFISEER
                     NEPPDIDVDFEHERREEVMQYIYAKYSGKRTALAAAVTSYRARSALREVAKAMGLSDD
                     VRSGLSSSIWGSSSSELGEREAKAGGLDKADPVTRQVMDLANEIMGFPRHLTQHVGGF
                     VITKDRLDEIVPIVKTAMDERKMVEWDKDDLDAVGLLKVDVLALGMLSCLRRAFDLLE
                     DHYEDAHDDYGRSYTLATLPPEDASVYDMICRADTLGVFQIESRAQMSMLPRLKPKEF
                     YDLVIEVAIVRPGPIQGDMVHPYLRRREQKEKVEYYKDELKAILGKTCGVPLFQEQAM
                     KIAIVAGGFKPGEADELRRAMATFKRTGTIHNYRDRMVNGMADKGYPRDFAERCFKQI
                     EGFGEYGFPESHAASFALLVYASCWFKTFYPDVFCAAILNSQPMGFYAPAQLVRDAQD
                     HGIEVREADVNFSGWDCALEEAAFDPGKVLARHGDMRGVIKSRHAVRLGFRQIKGLSD
                     KHMEAFVARRGNGYASVRDVWLRSGLDVAEIEKLAQADAFRSLGLDRRAALWAVRALD
                     GKSAAEKLPLFDRPGIALRELEPEAALPKMPPGEHVIHDYRSLGLSLKAHPVSFLRKR
                     LDGCGVMPNALLASAKNGRRVRVAGLVLVRQRPGKGNAIFLTLEDEKAVANIIFWPRV
                     FDRFRPIVMGAKFVCVTGKLQSETGVVHIVAEKIEDFTPWLAVLQEGAGGDGLASESG
                     EAQSNGHGDLSRQASALLDSGTQKVMPKGRNFQ"
     gene            complement(9404..9640)
                     /locus_tag="VGM46_12300"
     CDS             complement(9404..9640)
                     /locus_tag="VGM46_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEY4193419.1"
                     /translation="MATDERSGDRARVDPKLLELLACPLTKGPLAWDAERGELVSRLA
                     KLAYPVRDGIPIMLPSEARTLTIDDKPPRPRTSR"
     gene            complement(9664..10335)
                     /locus_tag="VGM46_12305"
     CDS             complement(9664..10335)
                     /locus_tag="VGM46_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006326887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEY4193420.1"
                     /translation="MRAGNANYRRNTDLPSTLPVFPLAGVLLLPGGRMPLNIFEPRYL
                     QMLDEAIAGSRLLGMIQPRLDGAVRPDGEPELGEVGCIGRIISFAESGDGRYLISLQG
                     VCRFRAARELPVKTPFRQCRIMPFLADLDEDQAGSDVDRPALLRAFRAYLKANELEAD
                     WEGVSRADNAMLVNALSMMAPFGPAEKQALLEAPDLKARAETLIAITEMAMARDSEDF
                     GSSLQ"
     gene            complement(10466..11455)
                     /gene="trxA"
                     /locus_tag="VGM46_12310"
     CDS             complement(10466..11455)
                     /gene="trxA"
                     /locus_tag="VGM46_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006326884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEY4193421.1"
                     /translation="MSSENPFGGMGGQYSTSVQYGTAPGANPASAATPSTGQAVDVIK
                     DTTTAGFAADVIQESRNQPVLVDFWAPWCGPCKQLTPALEKAVQAAGGRVKLVKMNID
                     DHPSIAGQLGIQSIPAIIAFKDGQPVDGFMGAIPESQIDAFIEKVGGKDDGQARIAAA
                     LAAAGEATTAGDTQTAADIYQAVLEQQPESVEAIAGLAGLLFEAGDSEGAQALLAQAP
                     DSAADAPALAALRAKMALAAQAAELGDPAEFERKLAENPKDHQARFDLAMIQSARGER
                     MAAADNLLAIIKADRAWQDDAARNQLLLFFEAWGMTDEATLSARRKLSSLLFS"
     gene            complement(11537..>11898)
                     /locus_tag="VGM46_12315"
     CDS             complement(11537..>11898)
                     /locus_tag="VGM46_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015314918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEY4193422.1"
                     /translation="NLFLKDKKDRFFLVTVGEDAVVDLKQIYHLIGASSRVSFGKPEM
                     LMELLGLVPGAVSVFGLINDAAHRVAIVLDAALMEHQTINAHPLTNEATTSIAAADLM
                     VFIKATGHEPIVLKVSA"
BASE COUNT         2049 a         3697 c         4023 g         2129 t
ORIGIN      
        1 cgggcaggcg caggggcttg gcgacaggct cggcgtgctg ctggtgcagt tgccgccgag
       61 cctgcgcttc gcgccggagg tcgcggaagg cttcttcgcc atgctgcgcg gcaaaaccat
      121 ggttggaatt gcctgtgagc cacgccatgc aagctggttt ggcaccgaag ccgacgcctt
      181 gctagcttcc catcagatcg cccgcgttgc cgccgatccg gccaggcacg agggcgcagg
      241 ccagccgggc ggatggccgg gccttgccta ttttcgcctg catggcgtgc cgcgcattta
      301 ctattcagac tatggcgacg aacggctacg ggaaatccgg caacgcatgg ccgcctgcca
      361 tgaagcgggc gcgaaagtct ggtgcatctt cgacaatacg gccgagggcc acgcgcttgg
      421 caacgccctt acgctcgacg gcgctgctcc gtgaggcttt gcgtcaaatc acgttcagtt
      481 cgcggaaggg gcgacgctcg gcaatgcgct gatagccgaa ggccgaacaa tcgacggtgc
      541 gataagcccc gtggacgatg aattcggcca gggacttgcc caccgccggg gcctgctgca
      601 agccgtggcc ggaaaagccg ttggcgaaga tgaagttcga cacgtccggg tgcgggccga
      661 tgaccgcgtt ctggtcgagc gcgttgtagt cgtaatggcc ggcccaggcg cgcgtcatct
      721 tgatcgcctc gaaggcggga atgcgcgtcg ccagcaccgg ccatatagtt tcctcgaaat
      781 ggctccagac cggctcgaaa tcggccggat cggcggcctg ctcgccttcc tcgacctccg
      841 cgccgccggc gatgtagacc gaaccttccg ggcggacata gatgccgctc ggatcgacca
      901 gcagcggcat gtcggcgaat ttgtcgcgcg cctcgaacac gaacaccgtg cgcttgcgcg
      961 gctccaccgg cagcgccagc ccggcaaggg ctgcgacctt gccgccattc ggcccggcgg
     1021 cgttgacgac gatgccggca tcgagcgttt cgccattgtc cagcgtcacg ccggtgacgc
     1081 gtgcgccgga gctggagatg ccggtgacgg tggcggtgat gagatcgatg ttcttgctgc
     1141 gcagcgcctt gcggaaaagc tggaggtagg catgggcgtc gaaccagccc tcgccgctta
     1201 ccccgtaggc gccggcagcg atgtcttcgg tcgacagcca ggcgaagcgc cgcgacaggg
     1261 tggcggcatc ttccagcacg atgtcggcgc cttcggccac ctgaacggca tggttggctt
     1321 tcaggacggg cagtccgttt tcgccggcga ggatcaggta gccgccttct cggaagccga
     1381 tgtcggcatc ttctccgaac tcctccttca gccgcttgaa caggccaagc gtgaactgtg
     1441 acaggcggat gttttgcgga atcgagaatt gctggcggat ggaagcggcc gaaagcgtcg
     1501 ttgccgaatg ggtgaactgg gggtcacgct cgaccagtgc gatggaaccg gaaaaacctt
     1561 ccttgcgcag gtaataagcg agcgatgagc cgacgatggc tccgccgatg atcacgatgt
     1621 cgtattgcgc catgtcgctc ttccgaagtc ggttggtcga aacagatgac agctttcgcc
     1681 accttgcggc aagcccttgc aagggccagc tttgacaggc aagaagacca gatgacgaca
     1741 gatggctttc gcccgcgccg ctcggtgctc tacgtgcccg ccgccaacga cagggcgctg
     1801 gccaagatgg cgacgcttgg ctgcgatgcc gtcatcatcg accttgagga ttccatcgcg
     1861 cccgccgaca agattttggc gcgcgagaag ctgaagggga ttttcgccag ccgcccacgg
     1921 cggcgttgcg agatggtggt gcgcatcaac gggcttgcta ccgaatgggg cgcggacgac
     1981 ctgctggccg ccgctgcttg cgagccggac ggcatccttc tgcccaaggt cgatacgccg
     2041 cgcgacgtgc tggaagccgg cgacgcgctc gacgacgcct ttgcgccgga cgaggtgaaa
     2101 ttgtgggcga tgatcgagac gccgaaggcg atgctcaaca tcggcgcgat cgccgagctt
     2161 ggtcgcgatc ctgcctcgcg gctgaactgt ttcgttgccg gaacgaacga tctcgtcaag
     2221 gaaaccggca tattggcgac gccggatcgt cgctacctgt tgccgtggct gatgcagatg
     2281 gtgctggcgg cgcgcgcggc cggtctcgac cttctggacg gggtcttcaa cgatttcggc
     2341 gatgcggacg gttttgcgcg tgaatgcacg gaggcggcca atatgggctt cgacggcaag
     2401 acgctcatcc atccggcgca ggtcgaaccg gcaaattcgg cgttcgcgcc ctcggcgcaa
     2461 gcagttgctc aggcaaaggc tatccacgat gcgtttgcgc ttgccggaaa tgcgaacaag
     2521 ggcgtgattt cgctggacgg caagatggtc gagcggctgc atctggccca ggcggaaaaa
     2581 ttactggcga aagcggccgc catcggggca tgatcgccaa aactcattat gggaccaaaa
     2641 gccaatgcgc ctctaccgct tcctcagcgc catcgacgat gccagcttct gtcacaaggt
     2701 gacggcggcg ctgaacaagg gctggcatct ctatggctcg ccgacctacg cctacgacaa
     2761 gaaagaaaag atcatgcgct gcgggcaggc cgtggtgaag gacattgaag gccaggaata
     2821 cgaggccggc aagaccaagc tgagcgactg gtaagacgcc agggaggcgg cgctactgcg
     2881 cctcttcctc aagcttttcc atatcgtcgt ccgaaaggcc gaaatggtgg ccgatctcgt
     2941 ggatcagcac atgggtgacg atgtcgccca gcgtctcctc gttctcggtc cagtaatcca
     3001 gcatcgcccg gcggtagagg gtgatgcggt tcgggccgtc gccggtctgc gggttccagc
     3061 gctcggcgat gccgcgtccc tcgaacaggc cgagcaggtc gaagggcgtt tccagcgata
     3121 ggtcgtccat gatctcgtcg gtggggaatt cggcgacctg gatgacgatt tcgccggtca
     3181 gcttgcgaaa ttcctcgggc agatgcgcgt aggtctccat ggcaaggaac tcgatctcct
     3241 ccagcgaggg cgaaagttcg tcgtgccagg tacgggtctt gtaaatgcgg gccattcgtt
     3301 ttccttgttg agccgcatat aggctctcgc gcctgaatcg gcaaatgacg ccggcttgat
     3361 ccggcgggga agggcggaga ggaataaatt cttttcgcaa cgcgggcaga ctctgcttga
     3421 ctccggggcg ggcctctgga atccataaga acataaagag aacagacttg ctggaagctg
     3481 gaccgtcatg gcgaaggatg ccgtggcgcg ggagatcgtt tttgccctgc gccgccagat
     3541 cgcgaaaatc gagggaacgc tgccggagtg ccttgaggcc cccgccaggg cggatatcac
     3601 cgtggtcagg cgcggcggcc aggccgaagg caatgacgcc ctgtccaatc gcgccctggc
     3661 cagtctcgcc ttgtcgactg gggcgggctg cctcgacgcg gcgcttggcg gcggcttgcc
     3721 acagggcgcg ctgaccgaaa tccacggtct cgaaacccgt gacgccggcg cggtcaccgg
     3781 tttcgtgctg gcgctggcgg gcctggcggt gggtgcggcg caaggcgagg ccgggcttgc
     3841 cacccctatc gtgtggatcg gcatgacgga gactttcggc gaggccggct ttccctatgc
     3901 gcgcgggctt cagtcgtcgt tcggcgtcgg tccggaatcg ctgctgtttt gcgaggctag
     3961 aaagctggcc gatgcgctgt ggatcgccga ggaagcggcg cggctgtcga catccgcagc
     4021 ggtcgttctc gaaattcgcg gcaacccgcg gcggctcgac ctgaccgcga caaggcggct
     4081 gcatgcaagg gcgcagcaaa ccgggcggcc gctgttcctg ctgcgacagg cagcccttgc
     4141 cgagccgacg gcggcgccgg tgcgcctcgt cgttttaccg gcgccttcgg gcttgcgcga
     4201 aacggttgcc ggtccgctca ccggttcgat cggcctgcct gccttcgccg tttcgatcag
     4261 caagagcgca tatgcctcga atggacaatt catactggag tggaaccccg atgaacgcat
     4321 attcatggaa cgaggcgcgg gggaacgaag tgcgggagaa atccgcgcaa aggatccttt
     4381 cgctgtggtt tccctacctc gccacggagc ggattctgcg gcagcgcctg gggcggtcgt
     4441 ggcgttcaag ccggccctcg gacgccaggc gcgcaagcct gccgctggtg atcagccacc
     4501 gcgaccgcaa cgcgcagcgc atcgcggcgc tcgacgagcg ggctgaggcg cttggcctga
     4561 agcgcggcat gggcatcgcc gatgcgcgcg ccatgcatcc ggcaatcgac atcgaggagg
     4621 ccgatgcgca ggccgacagg ttgctgctcg aagggcttgc cgactggtgc gatcgctata
     4681 cgccgctggt ggcgctggat ggggcggatg gattgttcct cgatattgcc ggctgcgcgc
     4741 atcttttcgg tggcgagagg gccatgctgg ataccatgct gacgcgcttt ttacagcagg
     4801 gcttcatcgc ccgtgcgggc atcgcctcgg cggcgggagc ggcgtgggcg gctgcgcgct
     4861 tcgccagcgg cagtatcgtt gccgtcggtg aggaggccgc acttttggcg ccgttgccgc
     4921 tttcggcgct gcgcatcgag ccggccgtgc gcgaaagcct ggaaagcgtc gggctgcgcc
     4981 gggtgggtgc ggtcatggcg acgccgcgtg cgccgcttgc ccgccgtttc ggcgcgctgt
     5041 tgctagagcg gctcgaccag gcgctcggcc ggagcgacga ggcggtatcg ccgcgcctgc
     5101 ccgccgcgcc gctttcggtg gagcgccatc tggctgagcc gataacccag atcgaggaga
     5161 tagagcgcct gacggcgagc ctgtcgcaga cgctgaaaca cgaccttgag cggcgcggcg
     5221 agggcgctcg cgcgcttggg ctgatgctgt ttcgcgtcga tggcgcagtc agccagatcg
     5281 ccatcggcac gtccaggcct ttgcgcgatc cgctgctgat ccagaaattg ttccatgagc
     5341 ggcttgccgc gaccgggcag gaatacgagg tcggctatgg cttcgatctg gtccgcctct
     5401 cggttctgtc ggttgcggcc ttcgacatgc agcagaccga cctgacgggg gagataaagg
     5461 atgcggaggc cgacattgcg cttttcgccg accgcgtccg ggcgcggctg ggggacaatg
     5521 ccgttcgccg gccgatatcc gtggcaagcc atctgccgga gcgcagcatc gcccttgtcg
     5581 ccttcacgga tgcgccggcg agcaagtcga agaatcgcgt tccgtccatc agcctatccg
     5641 agcggcccgt gcgcctgttc cgctcgcccg agccggtcga ggtgccggca accgaattgc
     5701 cggaggggcc gcctttgcat tttcgctgga ggcgcgcctt gtaccgggtt gtgcgcgccg
     5761 aaggcccgga gcggatcgcg ccggaatggt ggcgcgagag cattcccgac gccatggcga
     5821 aggagggcga ggacgcaggc gaggtcaaga acagggcggt cgcggagaag acggcatccc
     5881 tcacgcgcga ttattttcgt atcgaggatg ccgagggccg ccgttactgg ctctttcgcc
     5941 aggggcttta cgatgccggg cgcacatcgc cgcgctggtt catgcatggt atcttcgcat
     6001 gaagccggaa ccgccatgcg acacaccggg ggcgtcgcgc tacatcgaat tcggcgtgca
     6061 gtcgaatttc tcgttcctgc gcggggcttc gcggccggag gaactggtcg tggccgccag
     6121 cctgctcggc tatgccggca tcggccttgc cgaccgcaac agcgtggcgg gcgtcgtgcg
     6181 cgcatggagc cagtcgaagc atatcaagcc cgcggcggat gccgcgccgg cggcgtttgc
     6241 ctatcgtccg ggctgccgcc tcgttttctg cgacggcatg ccggatgtgc tcgcctatcc
     6301 gcgcgaccgc gaggggtggg ggcatctgtg ccgcatgctg acgcaggcca atctgcgcga
     6361 cgagaacgaa aagggcgcga cccttctgcg cggggccgac ctgctcgaat ggggcgagcg
     6421 catgtcgctt gccgtgctgg ccgatctatc gcgcgcaaaa gatcagatga cgcttctgcg
     6481 ccagatcaag gaccggttcg gcgaggccgt ccggctggcg gtggcgcctg cctatcgcgg
     6541 cgacgaccgc ttccgcatcg agcaggcggc ggcgatggcg tcggcgctgg gcttgcggct
     6601 gatggcgacc aacgacgtgc tctaccacac gagcgcgcgc cggccgttgc aggacgtgct
     6661 gactgcgatc cgcctcaatg tgccggtggc cgaagcaggg ctggaactgg ccgccaatgc
     6721 ggaacgccat ctgaagccgc cggcggagat ggcaaggctg tttcgccgct atccggaagc
     6781 ggtcgaagaa acatggcgct tcaagaagga gcttggcttt tcgcttggcg aattgcagca
     6841 caattatcca gatgagccga cgcgcgatgg cgcgaccccg cagcaggagc ttgagcggct
     6901 tgcgaaagag ggcgcggccg aacgctaccc cggcggggcg ccggcatctg taaccgagcg
     6961 catccgcgaa gaattgcggc tcatcgaaaa aatgggctat gcacgctatt ttctgacggt
     7021 gcacgacatc gtccggttcg cccgcagcca gggcattctg tgccagggcc gcggctccgc
     7081 cgccaattcg gtagtatgct tctgcatcgg catcacgccc gtcgggccgg acaagatcga
     7141 cacgctgttt gaacgcttca tttccgagga acgaaacgaa cctcccgata tcgacgtcga
     7201 tttcgagcat gagcggcgcg aggaggtgat gcaatatatt tacgccaaat acagcggcaa
     7261 gcgcaccgcg ctggccgccg ccgtcaccag ctatcgcgcc cgttcggcgc tgcgcgaggt
     7321 ggccaaggcg atgggcctgt cggacgatgt caggagcggg ctgtccagct cgatctgggg
     7381 atcgagttcg tcggaacttg gcgagaggga agcaaaggcg ggcggcctcg acaaggccga
     7441 tcccgtgaca aggcaggtga tggatctggc caacgagatc atgggatttc cgcgccatct
     7501 cacccagcat gtcggcggct tcgtcatcac caaagaccgg ctcgacgaga tcgtgcccat
     7561 cgtcaagacg gcgatggacg agcgcaagat ggtggaatgg gacaaggacg acctcgatgc
     7621 cgtcggcctg ctgaaggtcg atgtgctggc gctcggcatg ctgtcgtgcc tgcgccgcgc
     7681 cttcgacctg ctcgaagatc actacgagga cgcgcacgac gattatgggc gcagctacac
     7741 cctggcgacg ttgccgccgg aggatgccag cgtctacgac atgatctgcc gcgccgacac
     7801 gctcggcgtc ttccagatcg aatcgcgcgc ccagatgtcg atgctgcccc ggctcaagcc
     7861 gaaagaattc tacgatctcg tcatcgaggt ggcgatcgtg cgcccgggtc cgatccaggg
     7921 cgacatggtg catccctatc tgcgcagaag agaacaaaag gaaaaggtcg aatattacaa
     7981 ggatgagttg aaggcgatcc tcggcaagac ctgcggcgtc ccgttgttcc aggagcaggc
     8041 aatgaagatc gccatcgtcg ccgggggctt caagccgggc gaggccgacg aacttcgccg
     8101 cgccatggcg accttcaagc gcacgggcac gatccataat taccgggacc ggatggtcaa
     8161 cggcatggcc gataaaggct atccgcgcga cttcgccgag cgttgcttca agcagatcga
     8221 ggggttcggc gaatacggct ttccggaaag ccacgcggcg tccttcgcgt tgctggtcta
     8281 tgcatcctgc tggttcaaga cgttttatcc cgacgtgttc tgcgccgcga tcctcaattc
     8341 ccagccgatg ggcttttacg cgccggccca gcttgtccgc gatgcgcagg accatggcat
     8401 tgaagtccgc gaggccgacg tgaatttttc cgggtgggat tgcgcgctgg aagaggcggc
     8461 cttcgatccg ggcaaggtgc tggcgcgcca tggcgacatg cgcggggtca tcaaaagccg
     8521 ccatgccgtc cggctcggct ttcgccagat caagggcctg tcggacaagc acatggaggc
     8581 gttcgtagcg cggcgcggca acggctatgc ctcggtgcgc gatgtctggc tgcgttccgg
     8641 ccttgatgtc gccgagatcg agaagctggc gcaggcggac gccttccgct cgctcggcct
     8701 cgaccgtcgc gcggccttgt gggcggtgcg cgcgctggat ggaaagagcg cggccgaaaa
     8761 gctgcctttg ttcgaccggc ccggcattgc cctgcgcgag ctggagccgg aggcggcgct
     8821 gccgaaaatg ccgcccggcg agcatgtcat ccacgactat cgctcgcttg gcctgtcgct
     8881 caaggcgcat ccggtcagtt ttctgcgcaa gcggctcgat ggatgcggcg tcatgccgaa
     8941 tgcgctgctg gcttcggcaa agaacggcag gcgcgtgcgc gtcgccgggc tggtgctggt
     9001 gcggcagcgg cccggcaagg gcaatgccat cttcctcacg ctggaggatg agaaggcggt
     9061 tgccaacatc atcttctggc cgcgcgtatt cgatcgtttc cggccgatcg tcatgggcgc
     9121 gaaattcgtc tgcgttaccg gcaagttgca gtcggaaacc ggcgtcgtgc atatcgtggc
     9181 cgaaaaaata gaggatttca cgccctggct tgccgtgctt caggaggggg cgggcggcga
     9241 tggcctggcc tccgaaagtg gcgaggcgca gtcgaacggg catggcgacc tgtcccggca
     9301 ggcgtccgcc ctgctggaca gcggcacgca aaaggtcatg cccaagggcc gcaacttcca
     9361 atgaagcgct tggcggcacc ggccaaggcc tcgcggaagc cgtttagcgc gatgtgcggg
     9421 gacggggcgg cttgtcgtcg atcgtcagcg tgcgcgcttc cgacggcagc atgatcggga
     9481 taccgtcgcg caccgggtag gccaacttgg ccaaccgcga caccagttcg ccgcgctccg
     9541 cgtcccacgc aagcggtccc ttcgtcagcg ggcaggccag cagttcaagc agcttcggat
     9601 cgaccctggc cctatcgccg cttcgctcgt ccgtcgccat tgccttcgtc cttccgcgga
     9661 attctactgg aggctggagc cgaaatcctc gctgtcgcgc gccatcgcca tttcggtgat
     9721 ggcgatcaac gtttcggcgc gcgccttgag gtcgggcgct tcgagcagcg cctgcttttc
     9781 ggccgggccg aacggcgcca tcatcgacag ggcattgacc agcatggcgt tgtcggcgcg
     9841 gctgacgccc tcccaatccg cctccagctc atttgccttg aggtaggcgc gaaacgcccg
     9901 cagaagggcc ggccggtcga catccgagcc ggcctgatcc tcgtcgaggt cggccagaaa
     9961 cggcattatg cggcattgcc ggaaaggcgt cttgaccggc agctcgcgcg ccgcgcgaaa
    10021 acggcagacg ccctgcaatg aaatcaggta gcggccatcg ccgctctcgg caaaggaaat
    10081 gatgcggccg atgcagccga cctcgccaag ctcaggctcg ccgtccgggc gcaccgcccc
    10141 gtcaaggcgg ggctggatca tgcccagcag acgcgatccg gcgatcgctt cgtccagcat
    10201 ctgtaggtaa cgcggttcga atatgttgag cggcatgcgc ccgcccggca gcaggagaac
    10261 gccggcaagc ggaaaaacgg gcaaggtcga cggcagatcc gtgtttcggc gataattggc
    10321 gtttcccgcc cgcatcgtct ggctccactc ccttccggcg cggcgtcgga tcaggcatac
    10381 ccgatgcgac cggctgtatt gtccctgcct tatgcatcat gtggcgcggc ggcggccatc
    10441 agcaaggccg cgccgccaca atgccttacg aaaatagcag agacgacagt ttacggcgcg
    10501 ccgaaagcgt cgcctcgtcg gtcatgcccc atgcctcgaa gaaaagcagc agctggttgc
    10561 gcgccgcatc gtcttgccat gcgcggtcgg ccttgatgat ggccagcaga ttgtcggccg
    10621 ccgccatgcg ctcgccgcgg gcgctctgga tcatggcgag gtcgaagcgg gcctgatggt
    10681 ccttcgggtt ttcggccagc ttgcgctcga actcggccgg atcgccaagc tcggcagcct
    10741 gggcggcaag cgccattttc gcccgcagcg cagccagcgc cggcgcatcg gcagcggaat
    10801 ccggggcctg cgccaacagc gcttgcgcgc cctcgctgtc gccggcctcg aacagcagcc
    10861 cggccagccc ggcaatcgcc tcgaccgatt ccggctgctg ttccagcacc gcctgataga
    10921 tgtcggcggc cgtttgcgta tcgcccgccg tggtcgcctc gccggccgcc gccagcgcag
    10981 cggcaatgcg ggcctggccg tcatccttgc cgccgacctt ctcgatgaag gcgtcgatct
    11041 ggctttccgg tatggcgccc atgaaaccgt cgaccggctg gccgtccttg aaggcgatga
    11101 tggcgggaat ggactggatg ccaagctggc cggcgatgga gggatggtcg tcgatgttca
    11161 tcttgaccag cttgacgcgg ccgccggctg cctgcaccgc cttttcaagc gctggcgtaa
    11221 gctgcttgca gggtccgcac cacggcgccc agaaatcgac cagcaccggc tggttgcgcg
    11281 attcctggat cacgtcggcg gcaaagccgg cggtcgtggt gtccttgatc acatcgacgg
    11341 cctggccggt ggaaggcgtt gcagcggacg ccgggttggc gcctggagcc gtgccgtact
    11401 ggacgcttgt cgaatattgg ccgcccatgc cgccgaaggg gttttcgctg ctcatatcaa
    11461 tcgtcctttc ggtcgcgcgc cgcatcggtt catcgcgaca ttgtcgtcca agccatcgct
    11521 ggccaccatg tggcgatcac gccgagactt tcaagacaat aggttcatgt cccgtcgcct
    11581 tgatgaaaac catcagatcg gcggcggcaa tcgaggtcgt cgcctcgttg gtgagcggat
    11641 gggcgttgat ggtttgatgt tccatcaggg ccgcgtcgag tacgatggcg acgcggtgcg
    11701 cggcatcgtt gatcaggccg aaaaccgaga ccgcgcccgg cacgagaccc agaagctcca
    11761 tcagcatctc gggcttgccg aaggagaccc ggctcgaagc gccgatcagg tgatagattt
    11821 gcttgagatc gacaacggca tcctcgccga cggtgacgag aaaaaaccgg tccttcttgt
    11881 ccttgaggaa caggttct
//
LOCUS       DASYCS010000397        17557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_400650,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000397 DASYCS010000000
VERSION     DASYCS010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 17557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17557
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400650"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..462)
                     /gene="rbfA"
                     /locus_tag="VGM46_12320"
     CDS             complement(49..462)
                     /gene="rbfA"
                     /locus_tag="VGM46_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015314005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEY4193423.1"
                     /translation="MPKNSPAGPSQRMLRVGEQVRHALSETLQRGEILDPLIETTVIS
                     VSEVRMSPDLKIATAFVSPLGAKDADAVVEALNKHAKFLRGRASSALRQMKYMPEFRF
                     RLDTSFDNFAKINDLLKSPEVARDLGQDEDGEDKE"
     gene            complement(648..3230)
                     /gene="infB"
                     /locus_tag="VGM46_12325"
     CDS             complement(648..3230)
                     /gene="infB"
                     /locus_tag="VGM46_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013891267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEY4193424.1"
                     /translation="MSDTKSDDDKTLGVKKTLTLRRPGVEQGVVRQSFSHGRTKQVVV
                     ETKKRKFSMPGDKPEQAAPPASVFAPKPAPQPVVAAPVVQEAPKAPPPAPAPVERAAV
                     VLNDLSRQEMEARRRALEGSQVRDQEDRVRAAEDAKRRAEEDERRKREREESARRQAE
                     EEARLQTEAESRRRAEEEARRRAPQAADMVAADDEEAAKPKRVGVSAGVPVRRPVTPE
                     VARPAKPTKSEEDRRRGKLTLNSALSDEDARARSLSSMRRRQEKFKRAMHNEPREKVM
                     REVILPETITIQELAQRMSERAVDVVKYFMKQGQILKPGDIIDADTAELVATEFGHTV
                     RRVAESDIEEGLFNIADNTEDLVSRPPVVTIMGHVDHGKTSLLDAIRNANVVSGEAGG
                     ITQHIGAYQVEKNGQKITFIDTPGHAAFTAMRARGAQATDIAILVVAADDSVMPQTIE
                     SISHAKAAGVPIIVAINKIDKHDADAQKVRSELLRHEVFVESMGGEVLDVEVSAKQGT
                     NIDKLLEAILLQAEILDLKANPDRTAEGVVIEAQLDKGRGPVATVLVQTGTLMPGDIL
                     VAGNEWGRVRALVTDRGAHIKEAPPSMPVEVLGLQGTPQAGDRFAVVNNEARAREITE
                     YRQRIAHDKAVARNAGQRGSLEQMMSQLQNSGLKEFPLVIKGDVQGSIEAIVAALDKL
                     GTDEVRARIVHAGAGGITESDVSLALTSGAAMIGFNVRANPQARAAATAAGIDIRYYN
                     IIYNLVDDVKAAMSGLLSPERRETFIGNAEILEIFDITKVGKIAGCRVTEGKVERGAG
                     VRLIRDNVVIHEGTLKTLKRFKDEVAEVPSGQECGMAFQNYEDMRAGDVIECFRVEMV
                     TRTL"
     gene            complement(3227..3904)
                     /locus_tag="VGM46_12330"
     CDS             complement(3227..3904)
                     /locus_tag="VGM46_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006337977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEY4193425.1"
                     /translation="MAHNPRSEEMNDRTCIVTRKQAEPDDLIRFVVGPDKVVVPDIKR
                     SLPGRGCWVTADRLHMDKAAAKNLFARAFKAQVTVPDDLGAMVDGLLAKSVLGMLGMA
                     RKAGAISLGATKVESAVRSGLALFVTHALEASEDGVRKISQARRATVHLGGPAVRAYK
                     LFSEADLSLALGGTNVIHAAVLAGDAGKAVLKRMVALDRYRGGSPDDLAMLAAVADED
                     DAAEDME"
     gene            complement(3916..5508)
                     /gene="nusA"
                     /locus_tag="VGM46_12335"
     CDS             complement(3916..5508)
                     /gene="nusA"
                     /locus_tag="VGM46_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEY4193426.1"
                     /translation="MVVSANRLELLQIADAVAREKSIDKQIVIDAMADAIQKAARSRY
                     GQETNIRADINPNTGEMKLQRLMEVVEKVDDYAIQIALSTARERNPDAQLGDFIAEQL
                     PPMDFGRIAAQSAKQVIVQKVREAERDRQYDEYKDRIGEIINGTVKRVEYGNVIVDLG
                     RGEAIIRRDELIPRENYKYGDRVRAYVYDVRREQRGPQIFLSRTHPQFMAKLFTMEVP
                     EIYDGIIEIKSVARDPGSRAKIAVISRDSSIDPVGACVGMRGSRVQAVVGELQGEKID
                     IIPWSPSAASFIVNALQPAEVAKVVLDEDAERIEVVVPDDQLSLAIGRRGQNVRLASQ
                     LTGWDIDILTEQEESERRQKEFVERSALFMDALDVDEMVGQVLASEGFTSVEEVAYVE
                     SEEIASIDGFDEDTASEIQQRAREYLEKIEAEHDDKRKALGVEDELRELPGITTAMMV
                     TLGEDGVKTIEDFAGYAVDDLTGWKERKDGETKVFAGVLANHGVSRADAEQMVLAARL
                     KAGWITEDELVAEETADEAVGA"
     gene            complement(5557..6192)
                     /gene="rimP"
                     /locus_tag="VGM46_12340"
     CDS             complement(5557..6192)
                     /gene="rimP"
                     /locus_tag="VGM46_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEY4193427.1"
                     /translation="MTASEDMGDDRIIRESGIDARVAMIIQPVLRAIGFRLVRVQLSG
                     QNGMTLQVMAEREDGTMTVEDCEEVSRAVSPALDVDDPIEKAYHLEVSSPGIDRPLVR
                     KSDFTTWLGHLVKMETSILVTTKKRFKGKIEEADADGVVIRADKVAYGEEPMVRVPYD
                     AIAEARLILTDDLIRDALSKDNRARKEAKKHRGEPDDDAPEAAEDENELEM"
     gene            6517..6681
                     /locus_tag="VGM46_12345"
     CDS             6517..6681
                     /locus_tag="VGM46_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016557079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193428.1"
                     /translation="MTTTIRNSTFGRLFGSLGSAIAVAIAVDAGRRPLARDLEALGID
                     SREFAKIRRF"
     gene            complement(6722..8239)
                     /locus_tag="VGM46_12350"
     CDS             complement(6722..8239)
                     /locus_tag="VGM46_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEY4193429.1"
                     /translation="MDTFSLLAHGFLVALEPMNLFYALVGVTLGTAVGVLPGIGPALT
                     VALLLPVTYKLDPAGSLIMFAGIYYGGMYGGSTTSILLNTPGESASIVTALEGNKMAR
                     KGRGGPALATAAIGSFVAGLIATLGLALIAPTIVKMALSFGPAEYFALMILAFMTVSA
                     AFGDSALRGLTALFLGLALGLIGIDLQTGQARLAFGVPDLLDGIEVTTLAVAMFAIGE
                     TLSIAASMNIDDDKVESVKGSVWMSREDWSRSWKPWLRGTLIGFPIGAMPAGGAEIGT
                     FLSYSAEKQLARKPEEFGHGAIEGVAGPEAANNASAAGTLVPLLTLGLPTTATAAIML
                     AGFQQFGLQPGPLLFTNNSQLVWGLIASLLVANFMLLVLNLPLIGLWVKLLTIPKHWL
                     YPGILVFATLGTIGANPSVFELGILLVCGLLGYGLRRFGYPIAPVVIGLILGPMAEQQ
                     LRRALAISQGDVTVLFHSWIAIGLWTLAIAAVVVPMIMRARGKGQMLAQMAGDED"
     gene            complement(8259..8762)
                     /locus_tag="VGM46_12355"
     CDS             complement(8259..8762)
                     /locus_tag="VGM46_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008122416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEY4193430.1"
                     /translation="MSQQPSENSAPARAGTLDKAVLAIAAGLALVAVIIAWNTALMGT
                     AGSYSRVGPTTFPYVIAAVLFVLAVFTAVAGLRGTFPKREPEAIAPVLMIVAGLAGQM
                     LLLKAAGFSIATGVLFAATARAFGRGPLWVTFPIGVVLSFIVWFIFSKGLQLTLPAGP
                     LEKLITS"
     gene            complement(8759..9706)
                     /locus_tag="VGM46_12360"
     CDS             complement(8759..9706)
                     /locus_tag="VGM46_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006469177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEY4193431.1"
                     /translation="MKKLTVILSAACMFASASAFAADYKIMAPAAPGGGWDQTARAMQ
                     TALQDEKISSNVQVNNVPGAGGTIGLAQFINNGAGDPSQLIVGGYVMVGAILTNNSPV
                     TLDKVTPVARLTGEFEVVVVPTASDIKDMAGLVAKLKADPGAVSWAGGSAGGVDHIAA
                     GLIAKASSVDPTKVNYIAFSGGGEALAAILGGQVTVGISGYGEFESQIKAGQLRLIGI
                     SSDQKVEGIDAPTFKEGGVDVGIQNWRMVAAAPGITDEQKAAIGADIEKMVHSASWKK
                     ILADKGWADTYLAGDDFAKQLKADTDSTESILKDIGLVK"
     gene            complement(9885..10586)
                     /locus_tag="VGM46_12365"
     CDS             complement(9885..10586)
                     /locus_tag="VGM46_12365"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009449333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine(46)-N(7))-methyltransferase TrmB"
                     /protein_id="HEY4193432.1"
                     /translation="MAEQSRPSRATEAFFGRRRGRAVRPLQATALENGFKAYRLDLAS
                     PSPTDLKELFAVRTSSVRLEIGFGGGEHLMHRASTEPAIGFIGVEPFVNGMAKMMMAV
                     EKTPLANLRVYDDDATGLLDWLPQASLDGIDLLYPDPWPKKKHWKRRFVSVTNLDRFA
                     RVLKPGGLFRFASDIDTYVNWTLQHCRVHHAFQWQAGDAADWNMPYAGWPGTRYEAKA
                     LREGRQPAYLTFRRV"
     gene            complement(10603..11865)
                     /gene="metK"
                     /locus_tag="VGM46_12370"
     CDS             complement(10603..11865)
                     /gene="metK"
                     /locus_tag="VGM46_12370"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006090594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEY4193433.1"
                     /translation="MRQSYFFTSESVSEGHPDKVCDRISDEIVDLVYREARKSGMDPW
                     DVRVACETLATTNRVVIAGEVRVPKSLLKTDKQGNVLTDAGDFPIINPSKFKSAARKA
                     IRDIGYEQAGFHWKKAKIDILLHGQSADIAQGVDNASDRQGDEGAGDQGIMFGYACTE
                     TPDLMPAPLYYAHKILELLAAARHTGTGDVAKLGPDAKSQVTVRYENGKAAEVTQIVL
                     STQHMDESWDSKKVRAVVEPYIREALGSLPIAPDCNWYINPTGKFVIGGPDGDTGLTG
                     RKIIVDTYGGSSPHGGGAFSGKDTTKVDRSAAYAARYLAKNVVAAKLADRCTIQLSYA
                     IGVAQPLSVYVDLHGTGKVDEAKLEDALRTVMDLSPSGIRRHLDLNKPIYAKTSAYGH
                     FGRKAGRDGSFSWEKTDLAKALKDAVAA"
     gene            complement(12078..12494)
                     /locus_tag="VGM46_12375"
     CDS             complement(12078..12494)
                     /locus_tag="VGM46_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013635375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY4193434.1"
                     /translation="MIEENKKKPNPIDIHVGSRIRLRRNMLGMSQEKLGESLGITFQQ
                     IQKYEKGTNRVGASRLQAIASIIGVPVAFFFEDAPGQESVGNRGFSEDASMNFAVEFC
                     STPEGLQLNRGFVKIADPKVRRRIIDLVKSLSGDAD"
     gene            complement(12618..14210)
                     /gene="lnt"
                     /locus_tag="VGM46_12380"
     CDS             complement(12618..14210)
                     /gene="lnt"
                     /locus_tag="VGM46_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006337984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEY4193435.1"
                     /translation="MQRLAGRIILLSGWKRSLTAFLAGALAVLAQAPYDFFAVCFVSF
                     PVLVWLLDGATGELSDGWLKRLRPGFAIGWWFGFGYFLAGLWWIGEALLVEADSFAWA
                     LPFAVIGIPFALAFFYGFATVVARLCWSSDIGRIAALAVGFGLAEWLRGFLFTGFPWN
                     AVGYAAMPVPLLMQSVSATGMVGMNALAVFVFALPALLASRRHLVLGAALLAALVAAH
                     VGFGYIRLTEAADPEAKSLDVRIVQPNVDLSEKWDAAVRDRIFATMMELSAKAPEPGH
                     PMPQLVIWPETSVPFLFTQRPDALAALGNILAEGQMLLAGIVREEGGAAADSDARYYN
                     SVVAINDKGEIVDAVDKVHLVPFGEYLPFADLLRRFGVEQLVAGPMNFSAGNNRHAIT
                     VPGGIRALPFICYEVIFPDLVAVDAASSGLIVNVTNDAWFGDTPGPYQHFRQAQVRAV
                     ENGLPLLRGANNGISAAIDANGRIIDALAINKRGIIDVHLPLSRRAVVSANQRRINGL
                     LIILLFACVAGGLNVRQRLRVN"
     gene            complement(14321..15397)
                     /locus_tag="VGM46_12385"
     CDS             complement(14321..15397)
                     /locus_tag="VGM46_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019863065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEY4193436.1"
                     /translation="MNDKAEIAARPEAGSVGNASDKSEDDSGPSTTQAAGALDHAHTG
                     PSLFQRVLGLFRQRNGTTLREEIADALAETASDAESFSPGERAMLNNILRLREVRVED
                     VMVPRADIEAVEIGTTLGELLGLFEQSGHSRMPVYSETLDDPRGMVHIRDVLAHITKL
                     ARVRRGRPTKKAPALSTFDLAKVDLARTIDDLNLIRPVLFVPPSMLASDLMGRMQATR
                     TQMALVIDEYGGTDGLASLEDIVEMVVGDIEDEHDDDEPMITQAGDGIFIVDGKAEID
                     DVAKMIGGEFAAGEHGESVDTIGGMIFNTLGRVPARGEVVQAIPGFEFHVLDADPRRV
                     KRVRIVHSLKAERRRRAAVRTEQA"
     gene            complement(15426..15917)
                     /gene="ybeY"
                     /locus_tag="VGM46_12390"
     CDS             complement(15426..15917)
                     /gene="ybeY"
                     /locus_tag="VGM46_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002969509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEY4193437.1"
                     /translation="MEARSISNPPVEIDLYVEAGAWPDEAALLLLVEKAVAAAFAETG
                     TKGTSELSIVFSDDAHIQALNAGWRGKDKPTNVLSFPAFPPSRGSLMPPMLGDIVLAA
                     ETVARESQLENKPLEHHISHLVIHGLLHLLGHDHETDAEAEEMEAIERAALARLAIPD
                     PYA"
     gene            complement(15940..17007)
                     /locus_tag="VGM46_12395"
     CDS             complement(15940..17007)
                     /locus_tag="VGM46_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010913308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEY4193438.1"
                     /translation="MGPADEERRLSPAELKDLPPSGASDMAHIVLTFDNNKLASALYG
                     QFDENLARLEQKLGVDIRSRGNQLTIKGSAGAAEQARRALDSLYDILQKGANIGQSDV
                     DGAVRMAIAADDQLTLPTLERKGKMAAAQIATRKKTIYARSLNQDAYMRALERSELVF
                     GIGPAGTGKTYLAVAHAAMLLERGMVERIILSRPAVEAGERLGFLPGDMKEKVDPYLR
                     PLYDALYDMMPPDKVERAIAAEVIEIAPLAFMRGRTLAHAAVILDEAQNTTPMQMKMF
                     LTRLGENSRMIVTGDPTQIDLPPNTKSGLVEALRVLDGVAGAVTVRFNDGDVVRHPLV
                     AEIVRAYERDGKLARGLSTET"
     gene            complement(16991..>17557)
                     /locus_tag="VGM46_12400"
     CDS             complement(16991..>17557)
                     /locus_tag="VGM46_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010657764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY4193439.1"
                     /translation="LHLPVQSGSDRILKMMNRRHTASDYLKLLDRVRAARADIALSGD
                     FIVGFPGETDADFEATMQLVRDVGYASAFSFKYSPRPGTPGAEMDGHVPEPVKDERLQ
                     RLQALLVEQQQAFTVGMVGKAMPVLIEKPGRQAGQRVGRSPWLQPVIVDEKAGEIGDI
                     IQVRITGTGFNSLHAELAADRSDDGPGR"
BASE COUNT         3145 a         5598 c         5497 g         3317 t
ORIGIN      
        1 ccacccagcc ggagatcggc cggcccttct tcttgccgcg acgcgccact attccttgtc
       61 ctcgccgtct tcgtcctgcc ccaggtcgcg cgccacttcc ggcgatttca aaagatcgtt
      121 gatcttggcg aaattgtcga agctggtatc cagccggaag cgaaactcgg gcatgtattt
      181 catctgccgc agcgccgaag aggcgcggcc gcgcaggaac ttcgcgtgct tgttcagcgc
      241 ctcgaccacc gcatcggcat ccttcgcgcc aagcggcgaa acgaaggcgg tggcgatctt
      301 caggtcgggc gacatgcgca cttccgacac ggaaatgacg gtggtttcga tcagcggatc
      361 aaggatttcg ccgcgctgta gcgtttccga cagcgcgtgg cggacctgct cgccaacgcg
      421 aagcatgcgc tgggacgggc cagccggtga gtttttgggc ataaaagtct ctttccagta
      481 cggattcgaa ccgtgggagc cagcccctta gcgccgttgc gccggcctgg ctactgtccg
      541 gccggattac ggccggccga tcattcatta actaccgtcg tgcggcatcc tcgcctgtgg
      601 ctgacgggag cggcggtttc agccaaaccg cccccgatct acatgcctca aagcgtgcgc
      661 gtgaccatct cgacgcggaa gcactcgatg acatcgccgg cgcgcatatc ctcgtagttc
      721 tggaaggcca tgccgcattc ctggccgctc ggcacttcgg ccacttcatc cttgaagcgc
      781 ttcagcgtct tcagcgtacc ctcgtggatg acgacattgt cgcggatcag gcgaacgccc
      841 gcgccgcgct cgaccttgcc ttcggtgaca cggcaaccgg cgatcttgcc gaccttggtg
      901 atatcgaaga tttcgaggat ttcggcattg ccgatgaacg tctcgcggcg ttccggcgat
      961 agcaggcccg acatggccgc cttcacgtca tccacgagat tgtagatgat gttgtagtag
     1021 cggatatcga tgccggcagc ggtggcggcg gcgcgcgcct gcggattggc gcgcacgttg
     1081 aagccgatca tggccgcacc ggacgtcagg gcaagcgaga catcgctttc ggtgatgccg
     1141 ccggcgcccg catggacgat gcgcgcacgc acctcgtcgg tgccaagctt gtcgagcgcg
     1201 gccacgatcg cctcgatcga accctgcacg tcgcccttga tgaccagcgg gaattccttc
     1261 aacccgctgt tttgcagctg cgacatcatc tgttcgagcg aaccgcgctg gccggcattg
     1321 cgggcaaccg ccttgtcgtg cgcgatgcgc tggcgatact cggtgatctc gcgggcgcgc
     1381 gcctcattgt tgaccaccgc gaaacggtcg ccagcctgag gtgtgccctg caagccaagc
     1441 acttcgaccg gcatcgaggg cggcgcttcc ttgatgtgcg cgccgcgatc ggtaaccagc
     1501 gcgcgcaccc tgccccactc attgccggcg acgagaatgt cgcccggcat cagcgtgccg
     1561 gtctgcacca gcacggtggc gacggggccg cggcccttgt cgagctgcgc ctcgatgacc
     1621 acgccttcgg cggtgcggtc gggattggcc ttgaggtcga ggatttcggc ctgaagcagg
     1681 atcgcttcga gcagcttgtc gatgttggtg ccctgcttgg ccgacacttc gacgtcaagc
     1741 acctcaccgc ccatggattc cacgaacact tcatggcgca acagttcgga acgaaccttc
     1801 tgcgcgtcgg cgtcgtgctt gtcgatcttg ttgatcgcca cgatgatcgg cacgccggca
     1861 gccttggcat ggctgatcga ttcaatggtc tgcggcatca cgctgtcgtc ggcggccacc
     1921 accagaatcg cgatatcggt ggcctgcgcg ccgcgcgctc gcatggcggt aaaggcggcg
     1981 tggccgggcg tatcgatgaa ggtgatcttc tggccgttct tctcgacctg ataggcgccg
     2041 atatgctggg tgataccgcc agcttcgccg gaaacgacat tggcgttgcg gatggcgtcg
     2101 agcagcgagg tcttgccgtg gtcgacgtga cccatgatcg tcaccaccgg cggccgggac
     2161 acaaggtcct cggtattgtc ggcgatgttg aacaggcctt cctcgatgtc ggactcggcg
     2221 acgcggcgga cggtgtggcc gaattcggtg gcaaccagtt cagccgtgtc ggcgtcgatg
     2281 atgtcgcccg gcttcaggat ctggccctgc ttcatgaaat acttgaccac atccaccgca
     2341 cgttcggaca tgcgctgggc tagttcctgg atggtgatgg tttcgggcag aatcacttcg
     2401 cgcataacct tttcgcgcgg ctcattgtgc atggcgcgct tgaatttctc ctggcggcgg
     2461 cgcatcgacg acagtgaacg ggcgcgcgca tcctcgtcgg aaagcgccga attcaaggtc
     2521 agcttgccgc ggcggcgatc ttcctcgctc ttggtcggct tggccgggcg cgccacttcg
     2581 ggggtgaccg gacggcgcac gggcacgccg gcgctgacgc ctacacgctt gggcttggcg
     2641 gcctcctcat cgtcggcggc aaccatatcg gcggcctgcg gcgcgcggcg gcgcgcttcc
     2701 tcctcggcgc ggcggcgcga ttccgcctcg gtctgcaacc gggcttcttc ctcagcctga
     2761 cggcgggcgg attcctcgcg ctcgcgcttg cggcgctcgt cttcctcggc acggcgcttg
     2821 gcatcctcgg cggcgcgcac gcggtcttcc tggtcgcgaa cctgcgaacc ttcgagcgcg
     2881 cggcggcggg cttccatttc ctggcgcgac aggtcgttca gcacgacagc ggcgcgctcc
     2941 acgggcgctg gcgcgggcgg cggggccttg ggggcctcct gaacaaccgg agcggccact
     3001 accggctgcg gcgccggctt tggtgcaaac acggacgcgg gtggcgccgc ctgctccggc
     3061 ttgtcgccgg gcatcgagaa tttgcgcttc ttggtttcga ccacgacctg cttggtgcgg
     3121 ccatgtgaaa agctctgacg cacaacgccc tgctccacgc ctggacgcct caacgtcaag
     3181 gtcttcttga cgcccaaggt cttgtcatcg tccgatttcg tatcgctcat tccatatcct
     3241 ctgcggcatc atcttcgtca gcaaccgccg caagcattgc cagatcatcc ggggatccgc
     3301 cccggtatcg gtcgagcgca accatgcgct tcaggaccgc cttgcccgca tcgcccgcga
     3361 gaacggcagc atgtatcaca tttgtgcccc ctaatgccaa actcaaatcc gcctcggaga
     3421 aaagtttgta ggcacggacg gcaggaccgc ccagatgcac cgtcgcccgc cgcgcctggc
     3481 tgatcttgcg aacgccgtcc tcggaagctt cgagcgcatg ggtaacgaaa agcgccaggc
     3541 ccgagcgcac cgcgctctcg accttggtgg cgccgagcga aatggccccg gccttgcggg
     3601 ccatgcccag cattcccagg acggatttgg cgagcagtcc atcgaccatc gcgcctaggt
     3661 cgtcaggcac ggtgacctgc gccttgaagg cgcgcgcaaa aaggttcttc gccgccgcct
     3721 tgtccatatg taggcggtcg gcggtcaccc agcaaccacg gccgggaaga cttctcttga
     3781 tatccgggac gacgacctta tccgggccga cgacgaaacg gatcagatca tccggttcgg
     3841 cctgcttgcg ggtaacgatg cacgtgcgat cgttcatctc ttcggaacgc gggttgtgtg
     3901 cgatgcggtt tcacctcacg caccgacagc ttcgtcggcc gtttcttcgg cgacaagctc
     3961 atcttcagtg atccagccag ccttcaggcg ggcggcaagc accatctgct cggcatcggc
     4021 gcgcgacacg ccatggttgg cgagcacgcc cgcaaacacc ttggtctcgc cgtccttgcg
     4081 ttccttccag ccggtcaggt catcgaccgc atagccggcg aaatcctcga tcgtcttcac
     4141 gccgtcctcg cccagcgtca ccatcatggc ggtggttatg cccggaagct cgcgcagttc
     4201 gtcctcgacg cccagcgcct tgcgcttgtc gtcgtgctcg gcctcgatct tttccagata
     4261 ttcgcgggcg cgctgctgga tttccgaagc ggtatcctcg tcgaagccgt cgatggaagc
     4321 gatttcctcg gactcgacat aggcaacttc ctcgacgctg gtgaagcctt cggaagccag
     4381 cacctggccg accatctcat ccacgtccag cgcatccatg aacagcgcgg aacgctcgac
     4441 gaattccttc tggcggcgtt cgctttcttc ctgctcggtc aggatatcga tatcccagcc
     4501 ggtcaattgc gaggcaaggc ggacattctg gccacggcgg ccaatggcca gcgaaagctg
     4561 gtcgtccggc accacgacct cgatgcgctc ggcgtcctcg tccagaacga ccttggcgac
     4621 ttccgccggt tgcagcgcat tgacgatgaa ggaggcggcc gagggcgacc acggaatgat
     4681 gtcgatcttt tcgccctgca attcgccgac gaccgcctgc acgcgcgaac cgcgcatacc
     4741 gacgcaggcg ccgaccggat caatggaact gtcgcgcgaa atcacggcga tcttggcgcg
     4801 cgagccgggg tcgcgggcga ccgacttgat ctcgatgatg ccgtcgtaaa tctccggcac
     4861 ttccatggtg aaaagcttgg ccatgaattg cggatgggtg cgcgacagga atatctgcgg
     4921 gccacgctgt tcgcggcgca cgtcatagac ataggcgcgg acgcggtcgc catatttgta
     4981 gttttcgcgc gggataagct cgtcgcggcg gatgatcgcc tcgccacgtc caagatcgac
     5041 gatgacattt ccatattcga cgcgcttgac ggtgccgttg atgatctcgc cgatgcggtc
     5101 cttatattcg tcatactggc ggtcgcgctc ggcctcgcgc accttctgga cgatgacctg
     5161 cttggcggac tgggcggcga tgcggccgaa atccatcggc ggcagctgtt cggcgatgaa
     5221 atcgccaagc tgggcgtcgg gattgcgttc gcgggcggtg gaaagcgcga tctggatggc
     5281 gtagtcgtcg accttctcga ccacttccat caggcgctgg agcttcatct cgccggtgtt
     5341 cgggttaatg tcggcgcgga tgttcgtctc ctggccgtag cgcgagcgcg cggccttctg
     5401 gatcgcatcg gccatcgcat cgatgacgat ctgcttgtcg atcgactttt cacgcgcgac
     5461 cgcatcggcg atttgcagca gttcaagcct gttggcgctt acaaccatcg tttttctccg
     5521 gagcttgtcg ccgaaccttc cggcccggct gctcgatcac atttcaagtt cgttttcgtc
     5581 ttccgcggct tccggcgcgt cgtcgtccgg ttcgccgcga tgcttcttgg cttccttgcg
     5641 cgcccggttg tccttcgaca gggcatcgcg gatcaaatcg tcggtcagga tcaaccgcgc
     5701 ctcggcaatc gcgtcataag gaacgcgcac catcggttcc tcgccatagg ccaccttgtc
     5761 ggcgcggatg acgacgccgt cggcatcggc ttcctcgatc ttgcccttga agcgcttctt
     5821 cgtggtgaca agaatggaag tttccatctt caccagatgg ccgagccagg tggtgaaatc
     5881 ggacttgcgc accagcggcc ggtcgatacc gggcgacgat acttcaagat ggtaggcttt
     5941 ctcgatcgga tcatcgacgt cgagggcagg cgacaccgcg cggctgacct cttcgcaatc
     6001 ctcgacagtc atggtgccgt cttcgcgctc ggccatgacc tgcaacgtca tgccgttctg
     6061 gccggaaagc tgcacccgca cgaggcgaaa gccgatggcg cgcagcaccg gctggatgat
     6121 catcgcaacg cgggcatcaa tgccgctttc gcggatgatg cggtcgtcgc ccatgtcttc
     6181 gcttgccgtc actgcaatcc tgtcctgttt tgatcctgat ccggcctgaa ccctggctgg
     6241 ctcgcgaccg ccgcaaacgg catccgacac cagtggcggc aaggtaataa aaaagagcgg
     6301 gaccgggtgg acccactctt cgtcataccg accaagaatt tgagcagaaa ctaaacccga
     6361 ttcagggaaa tttcaagccc cgtcttcagc cccgccgcct atccatgcag ccagggcatg
     6421 gccggcatgc agcaaagcct ctcaaacaag cctgcgcaac cgtctatatg ttgcagtgca
     6481 caacgattga attttcaata gacgcagggg tttatcatga ccaccaccat cagaaactcg
     6541 acattcggcc ggctgttcgg ttcgctcggc agcgcgattg cggttgccat cgccgtcgat
     6601 gcaggccgcc ggccgctggc gcgcgatctc gaagcgctcg gcatcgattc ccgcgaattc
     6661 gcgaaaatcc gccgcttctg atttcttgcg gggacaggca agggccggaa cgtcgacagg
     6721 cttagtcttc gtcgccggcc atctgggcaa gcatctgccc tttgccccgc gcacgcatga
     6781 tcatcggcac gaccacagcg gctatcgcca gcgtccacag gccgattgca atccacgagt
     6841 gaaatagcac ggtaacgtcg ccctggctga tggccagcgc gcggcgcagt tgctgttcgg
     6901 ccatcggccc gaggatcagc ccgatcacca ccggcgcaat cgggtagcca aagcggcgca
     6961 ggccatagcc aagcaggccg caaaccagca ggatgcccaa ttcgaacacc gacgggttgg
     7021 cgccgatggt gccaagcgtg gcgaagacca gaatgccggg gtaaagccag tgcttcggga
     7081 tggtcagcaa tttcacccac aggccgatta gtggcaggtt cagcaccagc agcatgaagt
     7141 tggcgaccag caggctggcg atcaggcccc agacgagttg ggagttgttg gtgaacagca
     7201 gcggtccggg ttgcaggccg aattgctgga agccggccag catgatggca gccgttgctg
     7261 tggtcggcag gccgagcgtc agcagcggca ccagcgtgcc ggcggccgag gcattgttgg
     7321 cggcttccgg tccggcgacg ccctcgatgg cgccatggcc gaattcctcc ggctttctcg
     7381 ccagttgctt ttctgccgaa taggacagga aggtgccgat ttcggcaccg cccgccggca
     7441 tggcgccgat gggaaagccg ataagcgttc cgcgcagcca cggcttccag gaccgcgacc
     7501 aatcctcgcg gctcatccag accgatccct tgaccgattc caccttgtcg tcgtcaatgt
     7561 tcatggaggc ggcgatgctc agcgtttcgc cgatggcgaa catggcgacc gccagcgttg
     7621 tcacctcgat gccgtcgagc agatcaggca cgccgaatgc caggcgcgcc tgcccggtct
     7681 gaaggtcgat gccgatcagc ccgagcgcca ggccgaggaa aagcgccgtc aggccacgca
     7741 gcgcggaatc gccgaaggcc gccgaaacgg tcatgaaggc gaggatcatc agcgcgaaat
     7801 attctgccgg cccgaaggac agcgccatct tgacgatggt gggcgcgatc agcgcaaggc
     7861 caagggtggc gatcagcccg gcaacgaacg agccgatggc ggcggtcgcc agcgccggac
     7921 cgcccctgcc cttgcgggcc attttgttgc cttccagcgc ggtgacgatg gaggcgcttt
     7981 cgcccggcgt gttcaacagg atcgacgtgg tggaaccacc atacatgccg ccataataga
     8041 tgccggcgaa catgatgagc gaacccgccg gatcgagctt gtaggtaacg ggcaacagga
     8101 gggcgaccgt cagcgccggc ccgatgcccg gaagcacgcc gacggcggtg ccgagcgtga
     8161 cgccgaccag cgcgtaaaac aggttcatcg gctcaagagc gaccagaaat ccgtgcgcga
     8221 ggagagagaa agtatccatg ccgcgttacc tggccatttc aggaggtgat cagtttttcg
     8281 agcggtccgg ccggcagcgt caattgcagg cccttggaga agatgaacca gacgatgaac
     8341 gacaagacga cgccgatggg aaacgtcacc cataacggcc cacggccgaa ggcgcgcgcc
     8401 gtcgcggcga agaggacgcc cgtggcgatt gaaaacccgg ccgccttaag cagcagcatc
     8461 tgcccggcaa ggccggcgac gatcatcaga accggggcga tcgcttccgg ttcccgcttg
     8521 gggaaggtgc cgcgcaaccc ggccaccgcc gtgaacacgg caagcacgaa cagcaccgcc
     8581 gctatcacat agggaaaagt tgtcgggccg acacgcgaat aggacccggc agtccccatc
     8641 agggcggtgt tccaggcgat gatgacggcg accagcgcca gccccgcagc aatggcaagc
     8701 acggccttgt ccaaggtgcc tgccctcgcc ggggccgaat tttcgctcgg ctgctggctc
     8761 atttgacgag gccgatgtcc ttcaggatgg attcggtcga gtcggtgtcg gccttgagct
     8821 gcttggcgaa atcatcgccg gccagatagg tgtcggccca gcccttgtcg gccaggattt
     8881 tcttccagct tgcggaatgc accatctttt cgatgtcggc gccgattgcc gccttctgct
     8941 cgtcggttat tcccggcgct gccgccacca tgcgccagtt ctggatcccc acgtcgacgc
     9001 cgccttcctt gaaggtcggc gcatcgatgc cctcgacctt ctggtcgctg gaaatgccga
     9061 tgaggcgcag ctggccggcc ttgatctgcg attcgaactc gccgtaaccg gaaatgccga
     9121 ccgtcacctg gccgccgaga atggccgcca gcgcttcgcc gccgccggaa aacgcgatgt
     9181 agttgacctt ggtgggatcg acggacgaag ccttggcgat cagcccggcc gcgatatgat
     9241 cgacgccgcc ggcggagccg ccggcccacg acaccgcgcc gggatcggcc ttcagcttgg
     9301 cgaccaggcc ggccatgtcc ttgatgtccg acgctgtcgg aacgacgacc acctcgaact
     9361 cgccggtcag gcgggcgacc ggcgtcacct tgtcgagcgt taccggcgaa ttgttggtca
     9421 ggatcgcgcc gaccatcaca taaccgccga cgatcaattg cgagggatcg ccagcaccgt
     9481 tgttgatgaa ctgggccagg ccgatggtgc cgccggcgcc cggaacgttg ttgacctgca
     9541 cgttggagga aatcttctcg tcctgaagcg cggtctgcat ggcgcgcgcg gtctggtccc
     9601 agccgccgcc gggggctgcc ggcgccatga tcttgtagtc ggcggcgaag gcgctcgcac
     9661 tggcgaacat gcaggcagca gacaagataa cggtcagttt tttcataatg ttccccagtt
     9721 ctcttgaatt tcttgacagt accaggcggt tccggcctga accgcccggc gacgccgatg
     9781 caggtccccg ccgctgacag atcggtcata gaaacttttg cgagcgcgcc ttgtcaacac
     9841 aatgttgaca agattcggaa atcgcttccg cgtcggccgg cgcctcagac gcgccggaat
     9901 gtcagataag cgggctggcg gccctcgcgc aaagccttgg cttcatagcg cgtgccgggc
     9961 cagccggcat agggcatgtt ccagtcggcg gcatcgccgg cctgccactg gaaggcatgg
    10021 tggacgcggc aatgttgcag cgtccagttg acataggtgt cgatgtcgga ggcaaagcga
    10081 aacaggccgc ccggcttcag gacgcgcgcg aagcggtcga ggttggtcac gctgacgaag
    10141 cgccgcttcc agtgcttttt cttcggccat ggatcgggat agagcaggtc gatgccgtcc
    10201 agcgaggctt gcggcagcca atcgaggaga ccggtggcgt cgtcgtcata gacgcgcaaa
    10261 ttggcgagcg gggtcttttc caccgccatc atcatcttgg ccatgccgtt gacgaacggc
    10321 tcgacgccga taaagccaat tgccggttcg gtacttgccc ggtgcatgag gtgctcgccg
    10381 ccgccgaagc cgatctccag acgcactgag gaggtccgga cggcgaacag ctccttcaga
    10441 tcggtgggcg aaggcgaggc cagatcgagc cggtaggcct tgaagccgtt ttccagcgcg
    10501 gtggcctgaa gcgggcgcac ggcgcggccg cgccgccgcc cgaaaaaggc ttcggtagcg
    10561 cggcttggcc tcgattgctc cgccatcacg ccggtcgggc catcaggccg cgaccgcatc
    10621 cttcagcgcc ttggcgaggt cggtcttctc ccacgagaaa gaaccgtcgc gcccagcctt
    10681 gcggccgaaa tggccatagg ccgaggtctt ggcgtagatc ggcttgttga ggtcgaggtg
    10741 gcggcggatg cccgatggcg acagatccat caccgtgcgc agcgcgtctt ccagcttggc
    10801 ctcgtcgacc ttgccggtgc cgtgcaggtc gacatagacc gacagcggct gggcgacgcc
    10861 gatggcatag gaaagctgga tcgtgcagcg gtcggccagc ttggccgcca cgacgttctt
    10921 ggcgaggtag cgcgccgcat aggccgccga acggtcgacc ttggtggtgt ccttgccgga
    10981 gaaagcgccg ccgccatgcg gcgaggaacc gccataggtg tcgacgatga tcttgcggcc
    11041 ggtaaggccg gtatcgccat ccggaccgcc aatgacgaac ttgccggtgg gattgatgta
    11101 ccagttgcaa tccggtgcga tcggcaagct gccgagggct tcgcggatat agggttcgac
    11161 aaccgcgcga accttcttgg agtcccagct ttcatccata tgctgggtgg acagcacgat
    11221 ctgcgtcacc tcggcggcct tgccgttttc gtagcgcacg gtgacctggc tcttggcgtc
    11281 ggggccaagc ttggcgacgt cgccggtgcc ggtgtggcgg gcggcggcca gcagttcgag
    11341 gatcttgtga gcgtagtaga gcggagccgg catcaggtcc ggcgtttcgg tgcaggcata
    11401 accgaacatg atgccctggt cgccggcgcc ctcgtcgccc tgcctgtcgg aggcattgtc
    11461 gacgccctgc gcgatatcgg ccgactggcc atgcagcaga atatcgatct tggccttctt
    11521 ccagtggaag ccggcctgct cgtagccgat atcgcggatg gccttgcgcg cggcggattt
    11581 gaacttggac ggattgatga tcgggaaatc gccggcatcg gtcaggacat tgccctgttt
    11641 gtcggtcttg agcagcgatt tgggcacccg cacctcgccc gcgatcacca ccctgttggt
    11701 ggtggccagc gtttcgcagg caacgcggac atcccacggg tccatgccgc tcttgcgggc
    11761 ctcgcgataa accagatcga cgatctcgtc ggagatgcga tcgcatacct tgtccggatg
    11821 gccttcagaa accgattcgg acgtgaaaaa gtagctctgc ctcatgggtg tcccctcttg
    11881 aaaatcgatc ggcaggctgc cgatttaggc gcgcaacgtg ttagcggggg cgtgcccttc
    11941 tcgtcaagca aggtttgccc tggttcagag cgtttccttc actggtttga tggatgcatc
    12001 tcgcacggta gcctgcctgg gcataagcca cggcgccatc gggcgggcgg caagcgcgta
    12061 tcaggccact atgccggtca gtcggcgtcg ccggaaagcg atttgacgag atcgatgatt
    12121 cggcggcgca ccttggggtc ggcgatcttg acgaagccgc gattcagttg aagcccttcc
    12181 ggcgtcgagc agaactcgac cgcgaaattc atggaggcgt cttcggaaaa accgcgattg
    12241 ccgacggatt cctggcccgg cgcgtcctcg aaaaagaagg cgaccggcac gccgattatc
    12301 gaggcgatcg cttgcagccg gctggcgccg acgcggttgg tgcccttttc gtatttctgt
    12361 atctgctgaa acgttatgcc gaggctttca cccagttttt cctggctcat tccaagcata
    12421 ttgcggcgaa gtctgatccg gcttcccacg tggatatcga tggggttcgg ctttttctta
    12481 ttttcttcta tcatcttttc ctcgccgaaa tttcccggca ttcttctgtt ttcaccccaa
    12541 aaataagccc gccgccagcg gccggccatt ttcctgaaca tagtcggcta caatatgagt
    12601 ttctaatata cgtcctgtca attcacccgt agcctttgcc ttacgttgag ccctcccgca
    12661 acacaggcaa aaagcagtat aatcagcaat ccgttaatgc gccgttggtt agccgaaacg
    12721 accgcacgcc tcgacagagg caaatgtaca tcgataattc cacgcttatt gatcgccaaa
    12781 gcgtcgataa tgcggccatt tgcgtcaatt gcggccgaaa tgccattatt ggccccgcgc
    12841 agcaagggca atccgttctc caccgcgcga acctgggcct gtcgaaaatg ctgataaggg
    12901 ccgggcgtgt ccccgaacca tgcgtcgttg gttacattca caataagccc gcttgacgct
    12961 gcgtcaactg ccaccagatc gggaaaaatc acttcgtagc agatgaaggg aagcgcgcgg
    13021 atgcctcccg gcacggtgat cgcgtgccgg ttgttgccgg cggagaaatt catcggacct
    13081 gcgaccagtt gttccacgcc aaaacgacgc agtaaatccg cgaaaggcag atattcgccg
    13141 aaaggcacca ggtgaacctt gtcgacggcg tcgacgatct cgcccttgtc gttgatggcg
    13201 acgaccgaat tatagtagcg cgcgtcggaa tccgcagccg cgccgccttc ttcgcgcacg
    13261 atcccggcca gcagcatctg gccctccgcc agtatattgc cgagcgccgc caatgcatcc
    13321 gggcgttggg taaacaggaa cggcaccgat gtttccggcc agattaccag ttgcggcatg
    13381 ggatggcccg gctcgggcgc cttggccgac agttccatca tggtcgcaaa gatgcggtcg
    13441 cgcaccgccg cgtcccactt ctcgctgagg tcgacattgg gctgcacgat ccgcacatcg
    13501 aggcttttcg cttcggggtc ggccgcttcg gtaagccgga tgtaaccgaa cccgacatga
    13561 gccgcgacga gggcggcgag cagggccgcg cccaaaacga ggtgccggcg cgaagcaagc
    13621 agcgccggca gcgcgaagac gaacacggcg agtgcgttca tgccgaccat tcccgtcgcc
    13681 gacacgcttt gcatcagcaa gggcaccggc atcgccgcat agccgaccgc gttccacggg
    13741 aagccggtga acagaaagcc gcgcagccat tcggcgaggc cgaagcccac ggccagcgcg
    13801 gcgatgcggc ctatgtcgct gctccagcag agcctggcca ccaccgtcgc gaaaccgtag
    13861 aaaaaggcca gcgcaaaggg aattccgatc acggcgaagg gcagcgccca ggcgaaactg
    13921 tcggcctcga ccagcagcgc ctcgccgatc caccacaggc cggccaggaa atagccgaag
    13981 ccgaaccacc agccgatggc gaagccgggc cgcagccgct tcagccagcc atcggacaac
    14041 tcgcccgtcg ccccatcgag cagccacacc agcaccggaa atgaaacgaa gcagaccgcg
    14101 aagaaatcgt agggcgcctg cgccagcacc gccaaggctc cggcaaggaa tgcggtgagc
    14161 gagcgcttcc accccgagag cagaattatc cgaccggcaa ggcgctgcat caaggtttcc
    14221 atgtcgttcg aatcacggcg tgaaatttaa caggccacgg cccgcgctcc aaacggcgaa
    14281 gcaagggtat ttcagacgcc agaacgcagc cggtgcggcg tcacgcctgt tcggtgcgca
    14341 ccgcagcgcg acggcggcgc tcggccttca ggctgtggac gatgcgcacg cgcttgacgc
    14401 ggcgcggatc ggcatcgagg acgtggaact cgaagccggg aatggcctgc accacttcgc
    14461 cacgggcggg cacgcggccc agcgtattga agatcatgcc gccgatggtg tcgacggatt
    14521 cgccatgttc gccggcggcg aattcgccgc cgatcatctt ggcgacatcg tcgatctcgg
    14581 ctttgccatc gacgatgaag atgccgtcgc cggcctgcgt gatcatcggc tcgtcgtcgt
    14641 catgctcgtc ctcgatgtcg ccgacgacca tctcgacgat gtcttccagc gaggcgaggc
    14701 cgtcggtgcc gccatattca tcgatgacca gcgccatctg ggtgcgcgtc gcctgcatcc
    14761 gccccatcag gtcggaggcg agcatggacg gcggcacgaa cagcaccggc cggatgaggt
    14821 tgagatcgtc gatggtgcgg gcaagatcga ccttggccag gtcgaaagtg ctgagcgccg
    14881 gcgccttctt cgtcggccgg ccccgccgga cgcgggcaag cttggtgata tgggccagca
    14941 cgtcgcggat gtgaaccatg ccgcgcggat cgtccagcgt ctcggaatag accggcatgc
    15001 gcgagtggcc ggattgttcg aacaggccga gcaactcgcc cagcgtcgtg cctatctcga
    15061 ccgcctcaat atcggcgcgc ggcaccatga catcctcgac gcgcacttcg cgcaaccgca
    15121 gaatgttgtt gagcatggcg cgttcgccag gcgaaaacga ttccgcgtcg cttgccgttt
    15181 cggccaaagc atcggcgatt tcctcgcgta aagtcgtgcc gttgcgctgc ctgaaaaggc
    15241 ccagcacgcg ctggaaaagc gaggggccgg tgtgcgcatg gtcaagcgcg ccagcggcct
    15301 gggtggtact gggacccgaa tcgtcttccg acttgtcgga agcgttgccc acgctgcctg
    15361 cctcgggacg ggcggcaatc tctgctttgt cgttcatcgt cgtccggtca atttctgttt
    15421 cggtgttacg cgtagggatc gggaatggca agccttgcaa gtgcggcgcg ctcgatggcc
    15481 tccatctcct ccgcctctgc gtcggtctcg tggtcatggc caagaaggtg cagcaggcca
    15541 tggattacaa gatggctgat gtggtgttcg agcggcttgt tttccagctg gctttcgcgc
    15601 gccaccgtct cggcggccag cacgatatcg cccagcatcg gcggcatcag gctgccccgc
    15661 gaaggcggaa acgccgggaa cgacagcacg ttggtcggct tgtccttgcc gcgccagccc
    15721 gcattgagcg cctggatatg ggcgtcgtcg gaaaagacga tgctgagttc cgacgtgcct
    15781 ttcgtgccgg tctcggcaaa ggcggcggct accgccttct cgaccagcag aagcagagcg
    15841 gcctcatccg gccacgcgcc ggcctcgaca taaaggtcga tctccaccgg cggattgctg
    15901 atcgaccgag cttccatcac atcaagcaat cgccccgcgt cacgtctcgg tgctcaggcc
    15961 gcgcgcaagc ttgccgtcgc gttcataggc ccggacgatc tcggcgacca gcggatggcg
    16021 caccacgtcg ccatcgttga agcgcaccgt caccgcaccg gcgacgccat cgagcacgcg
    16081 cagggcttcg acaaggccgg acttggtgtt tggcggcaga tcgatctgcg tcggatcgcc
    16141 ggtgacgatc atgcgcgaat tctcgccaag ccgggtcagg aacatcttca tctgcatcgg
    16201 tgtggtgttc tgcgcctcat ccagaatgac ggcggcatgc gccagcgtgc ggccgcgcat
    16261 gaaggcgagc ggcgcgattt cgatcacttc ggcggcgatg gcgcgctcga ccttgtcggg
    16321 aggcatcatg tcgtaaagcg cgtcatagag cggccgcagg tatggatcga ccttttcctt
    16381 catgtcgccg ggcaggaagc cgagacgctc gccggcctcg accgccggcc gcgacaggat
    16441 gatgcgttcg accatgccgc gttccagcag catcgccgca tgcgccaccg ccagataggt
    16501 cttgccggtg ccggcaggcc cgatgccgaa caccagttcg gaacgctcca gagcgcgcat
    16561 ataggcgtcc tggttgagcg agcgggcata gatcgtcttc ttgcgcgtgg ctatctgggc
    16621 cgcagccatc ttgcccttgc gctccagcgt cggcagcgtc agctgatcgt cggcggcgat
    16681 cgccatgcgc accgcgccat cgacatcgga ctggccgata ttcgcgcctt tctggagaat
    16741 gtcatagagg ctgtccaagg cgcggcgggc ctgttcggca gcgccggcgg aacccttgat
    16801 ggtcagctgg ttgccgcgcg agcgaatatc aacgccgagc ttctgttcga ggcgggccag
    16861 attttcatcg aactgtccgt aaagggcgct ggccagcttg ttgttgtcga aggtcaggac
    16921 gatgtgcgcc atgtcggagg cccccgatgg aggcagatcc ttgagttccg ctgggctcaa
    16981 ccgtctctcc tcatctgccg ggcccatcat cggacctatc ggcggccaat tccgcatgca
    17041 ggctgttgaa gcccgttccg gtgattcgga cctggataat gtcgcctatc tcgccggcct
    17101 tttcatcaac aataacgggt tgcagccaag gcgagcggcc aaccctctgg ccagcctgcc
    17161 ggcccggctt ttcgatcagc accggcatcg ccttgccgac cattccaacg gtgaaggcct
    17221 gctgttgttc caccagcagc gcctgcaacc tttgcagccg ctcatccttg acgggttccg
    17281 gcacgtggcc gtccatctcg gcgcccggcg tgccggggcg cggcgaatat ttgaaggaaa
    17341 aggcggaggc ataaccgacg tcgcggacaa gctgcatcgt cgcttcgaaa tcggcatccg
    17401 tctcgccggg aaagccgacg ataaagtcgc cggacagggc aatgtcagcg cgggcggcgc
    17461 gaacgcggtc gagcagcttc agataatcgc tggcggtatg gcggcggttc atcattttca
    17521 ggatacggtc cgaccccgat tgcaccggca ggtgcag
//
LOCUS       DASYCS010000398         7155 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3881166,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000398 DASYCS010000000
VERSION     DASYCS010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 7155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7155
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3881166"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..2531)
                     /locus_tag="VGM46_12405"
     CDS             complement(87..2531)
                     /locus_tag="VGM46_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006466698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HEY4193440.1"
                     /translation="MIRLIGYFFGIGTMLALLVAAGVAIYVGNLTKELPDYEVLAKYE
                     PPVTTRIHASDGSLMAEYAHERRLYLPIQAIPDRVKAAFMSAEDKNFYTHPGVDIMGI
                     GRAALRYATGGPMQGGSTITQQVAKNFLLTNERSIDRKIKEAILSFRIEQAYSKDRIL
                     ELYLNEIFFGLGAYGVAGAALTYFDKSVNELTVAEAAYLAALPKGPSNYHPFKHADRA
                     LERRNWVIGQMVDNGYVTREEGEKAIAEPLGVKARRNGTYLFAGEYFTEEVRREIIAR
                     YGEAALYEGGLSVRTTLDPQMQRLARKTMQNGLLKYDMLRGYRGPVTTIDASGDWGTA
                     LGAVKGLEDVPEWSLAVVLDSSATGLSIGLQPDRQASGELVKDRVEGTVSKEDMSFAM
                     RHIVDGKSVKAKSPADVLKAGDVVFVEKKDGADGAYQLRQVPQVEGGMVAMDPHTGRV
                     LAMVGGFSYAQSEFNRATQAMRQPGSSFKPIVYSAALDNGYTPASVIMDGPITIQSGT
                     TTWTPKNYDGRVAGPATLRSGIERSRNLMTVRLANDMGMKLVVEYAERFGVYDHLAPY
                     LPMALGSGETTVMRMVSAYSIMANGGKSIKPSLIDRIQDRYGKTVFKQDDRGCEGCNA
                     AEWQNQPEPELVDNSEQVLDPMTAYQITSMMEGVVQRGTGATINELGRHIAGKTGTTN
                     DEKDAWFIGYTPNLVLGLYMGYDQPKGLGHGATGGGLAAPIFKNFMRVALDGKPDVDF
                     KVPEGMKLIAINHKTGMRASEGEPGTIIEAFKPGTGPADSYWVIGMGADGSNAAGGAL
                     SPQATQAIQSGGGGLY"
     gene            complement(2528..2737)
                     /locus_tag="VGM46_12410"
     CDS             complement(2528..2737)
                     /locus_tag="VGM46_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193441.1"
                     /translation="MMLRCAYTTPAGFIAGPKNRNFGLPHFIHIMATWVRDCGSASFQ
                     NLARQNLARDAGLFRKTPNHGLERA"
     gene            complement(2763..4016)
                     /locus_tag="VGM46_12415"
     CDS             complement(2763..4016)
                     /locus_tag="VGM46_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006333203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEY4193442.1"
                     /translation="MATKNRRAQAIGHHGLAVLFLFVSAWLLALAPVARAADAPLQAL
                     SYKMAGDAVHMRIVMNFDREPDVKWFLLRGPNRMIIDMPATRFSLNARDVKSRGLVRR
                     VRYGALGADASRLVLTGKGPFVVDKFDILKNDDGSGYRVVADISAASEREFDEALAVQ
                     AQTTGSTVSADKSDRVGAQTGKADAPPHRFTIVIDPGHGGVDGGAESAKGTIEKNVTL
                     AFAKELREKLETSAKYDLFMTREDDEFLRLDDRVRIARQHEADLFISIHADTINVKGI
                     RGATVYTVSDKASDAEAQALADRENLSDQLAGIEMKNDSPEVTDILIDLIRRETHSFS
                     MTFANALVGKLSSTVGLINNPHRYAGFRVLKAPDVPSVLIELGYLSNDKDEEQLLDPV
                     WRGKAAQSIADAVALFASAKVGAGG"
     gene            4696..>7155
                     /locus_tag="VGM46_12420"
     CDS             4696..>7155
                     /locus_tag="VGM46_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008838047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEY4193443.1"
                     /translation="MPNKMLIDASHPEETRVVVVRGSRIEEFDFESQDKKQLKGNIYL
                     ARVTRVEPSLQAAFVEYGGNRHGFLAFSEIHPDYYQIPVADRQALLRAEAEEAEAEEN
                     EEADGEGRHDRDRGRRGRRRGGKGRERAEHHRHDAASSEAGSAEGGDEERANENGDEA
                     EDRGAPDAAEGQEAAAVENHDEHDNAAGEPSDGKSGSIAASVDADVVSEPAPAEETGE
                     ATSDDNDRGMLEEVHAANHDDHEIESVGAEDALDEVRNRRKPTRRQYQIQEVIKRRQI
                     LLVQVVKEERGNKGAAITTYLSLAGRYSVLMPNTARGGGISRKITNAQDRKRLKEVVA
                     DLEVPQGMGVILRTAGESRTKAEIKRDYEYLMRLWENVRNLTLQSTAPALVYEEGSLI
                     KRSVRDLYNKDIDEVLVSGEDGYREAKDFMRMLMPSHAKVVQPYRDTTPIFVRSGIEA
                     QLDRMLQPQVTLKSGGYIIINQTEALVAIDVNSGRSTKEHSIEDTALHTNLEAAEEVA
                     RQLRLRDLAGLIVIDFIDMEENRNNRAVEKRLKDHLKNDRARIQVGRISHFGLMEMSR
                     QRIRASVLESTMKPCPTCGGTGHVRSDSSVALAVVRAIEEFLLKDSKCHITVRTPAAT
                     ALYVLNHKRGTLVELESRFGLTVTIEADDTVGAQLYAISRGLVAEKPAGFVETRALPV
                     YDEPEEPEIVEEEDDEASEPVAEATPRQPDQAVRPTEEGEGRDRKRRRRRRRRGGKDR
                     ENGDASNQDAAGDDAVSDEAGYADVELDGDAPLEATAATEADPSEEAQAKKRRRGKRG
                     GKRSRQDDVEANAEGEAEAVAA"
BASE COUNT         1413 a         2294 c         2198 g         1250 t
ORIGIN      
        1 ggtcggcgcg atacataggg aggccgccgc cggctgtaaa gcaaaatggg ccggcgcaag
       61 gccagcccat ccgctcatga aaatcgtcag tagaggccgc cgccgccgga ctggatggcc
      121 tgcgtggctt gcggcgacag cgctccgcct gccgcattgg aaccgtcggc gcccatgccg
      181 atcacccaat agctgtcggc ggggccggtg cccggcttga acgcctcgat gatcgtaccc
      241 ggctcgcctt cgctcgcccg catgccggtc ttgtggttga tggcgatcag cttcatgcct
      301 tccggcacct tgaagtcgac atccggcttg ccgtccagcg ccacgcgcat gaagttcttg
      361 aagatcgggg ccgcgagacc gccgccggtc gcaccgtggc caagaccctt cggctggtcg
      421 taacccatgt agagaccgag aacgaggttt ggcgtgtaac cgatgaacca cgcgtctttt
      481 tcgtcgttgg tcgtgccggt cttgccggct atgtggcggc caagctcgtt gatggtggcg
      541 ccggtgccgc gctgcaccac accctccatc atcgaggtga tctggtaggc ggtcatcggg
      601 tcgagcacct gctcggaatt gtcgaccaat tccggctcgg gctgattctg ccactcggcc
      661 gcgttgcagc cttcgcagcc gcgatcgtcc tgcttgaaaa ccgtcttgcc gtagcgatcc
      721 tgaatccggt cgatcaacga cggcttgatc gatttgccgc cattggccat gatcgaatag
      781 gccgaaacca tccgcatcac cgtcgtctcg cccgaaccca gcgccatcgg caaatagggc
      841 gcgagatgat cgtagacgcc gaaacgctcg gcatattcaa cgaccagctt catgcccatg
      901 tcgttggcaa gccgcaccgt catcaaattg cgcgaacgct cgatgcccga gcgcaaggtc
      961 gccggtccgg ccacgcggcc gtcatagttc ttcggcgtcc aggtggtggt gccgctctgg
     1021 atggtgatcg gcccgtccat gatgacggaa gccggcgtgt agccattgtc gagcgccgcc
     1081 gaatagacga tcggcttgaa cgacgaaccc ggctgccgca tggcctgcgt cgcacgattg
     1141 aactcggact gcgcatagga aaagccgccg accatggcca gaacgcggcc cgtatgcggg
     1201 tccatggcaa ccatgccgcc ttccacctgc ggcacctgcc gcagttgata ggcgccgtcg
     1261 gccccatctt tcttttcgac gaagacgaca tcgcccgcct ttagcacatc ggccggcgac
     1321 ttggccttga ccgacttgcc atcgacgata tggcgcatgg caaagctcat atcctccttg
     1381 gaaaccgtgc cctcgacacg atccttgacc agttcgcctg acgcttgcct gtctggctgg
     1441 aggccgatgg aaaggccggt ggccgaactg tccagcacca cggccaggga ccattccggc
     1501 acgtcctcaa ggcccttgac cgcgccaagc gccgtgcccc agtcgcccga agcatcgatg
     1561 gtggtgaccg ggccgcgata gccgcgcagc atgtcgtatt tcaagaggcc gttctgcatc
     1621 gtcttgcggg caagacgctg catttgcgga tcgagcgtcg tgcgcaccga caaaccgcct
     1681 tcataaagcg ccgcctcgcc gtagcgggcg atgatctcgc ggcggacttc ctcggtgaaa
     1741 tactcgccgg caaaaagata ggtcccgttg cggcgcgcct tgacgccgag cggctcggct
     1801 atcgccttct cgccctcttc gcgcgtgaca tagccattat cgaccatctg gccgatgacc
     1861 cagttgcggc gctcaagcgc ccggtcggca tgcttgaagg gatggtagtt cgaaggcccc
     1921 ttcggcagcg ccgccagata ggccgcttcg gccaccgtca gttcattgac cgacttgtcg
     1981 aaataggtga gcgcggcgcc cgcaacgcca tacgcgccaa ggccaaagaa gatttcgttg
     2041 agatagagtt cgaggatgcg gtccttcgaa taggcctgct cgatgcggaa cgacaggatc
     2101 gcttccttga tcttgcggtc gatggagcgt tcgttggtca gcaggaagtt cttcgccacc
     2161 tgctgcgtga tggtcgagcc gccctgcatc gggccacccg tcgcatagcg cagcgcggcg
     2221 cggccgatac ccatgatgtc gacgccggga tgcgtgtaga aattcttgtc ctcggccgac
     2281 atgaaggccg ccttcacgcg gtctggaatg gcctggatgg gaagataaag ccggcgctca
     2341 tgcgcatatt cggccatcag cgaaccatcg gaagcgtgga tgcgcgtcgt taccggcggc
     2401 tcgtacttgg ccagcacttc atagtccggc agttctttcg tcagattgcc gacatagatc
     2461 gccacgcctg ccgcgacgag cagggcaagc atggtgccga tgccgaaaaa gtagccgatg
     2521 agacgaatca tgcccgctcc agtccatggt tcggcgtctt cctaaacaaa ccagcatccc
     2581 gcgcaaggtt ctggcgcgca aggttctgga acgaagccga tccgcaatcg cgcacccatg
     2641 tcgccatgat gtggataaaa tgcggcaagc cgaaatttcg gttcttgggg ccggcaataa
     2701 aaccggccgg tgttgtgtac gcgcaacgca gcatcatgcg gcatccttgc ggccagccca
     2761 attcagcctc ccgcgcccac cttggccgaa gcaaacagcg ccaccgcgtc ggctatgctt
     2821 tgggcggcct tgccgcgcca cacgggatcg agcaattgct cctcgtcctt gtcgttcgac
     2881 agatagccga gctcgatcag gaccgaaggc acgtcgggcg ccttgagcac cctgaacccg
     2941 gcatagcgat gcgggttgtt gataaggcct accgtactcg acaatttgcc caccagcgcg
     3001 ttggcgaagg tcatggagaa ggaatgcgtc tcacgccgga taaggtcaat caaaatgtcc
     3061 gtgacttcgg ggctgtcgtt cttcatttca atgccggcaa gctggtcgga taggttttcg
     3121 cggtcggcca gcgcttgcgc ctcggcgtcc gaagccttgt ccgaaacggt gtagacggtc
     3181 gccccacgta tgcccttcac gttgatcgta tcggcatgga tggagatgaa cagatcggct
     3241 tcatgctggc gcgcgatgcg cacgcggtcg tccagccgaa ggaactcgtc gtcctcgcgc
     3301 gtcatgaaca ggtcgtattt ggccgatgtt tcgagctttt cgcgcagttc cttggcgaac
     3361 gccagcgtca cgttcttttc gatggtgccc ttcgcgcttt cggcgccgcc atcgacgccg
     3421 ccatggccag gatcgatcac gatcgtgaag cgatgcggcg gcgcatcggc cttgccggtc
     3481 tgcgcgccga cgcggtcgct cttgtcggcg gaaaccgtcg atccggttgt ttgggcctgc
     3541 acggcaagcg cctcgtcgaa ttcccgctcg gacgccgccg aaatatccgc cacgacccgg
     3601 tagccgctgc catcgtcgtt tttgagaatg tcgaacttgt cgaccacgaa cggccccttg
     3661 cccgtcagca ccagcctgga agcatccgcg ccgagcgcgc cgtagcgcac gcgccgcacc
     3721 aaccccctcg acttgacgtc cctggcattg agcgaaaacc gcgtcgccgg catatcgatg
     3781 atcatccggt tcgggccgcg cagcaaaaac cacttcacgt ccggctcgcg gtcgaaattc
     3841 atgacgatgc gcatatgcac ggcgtcgccc gccatcttgt aagacagcgc ctgcaacggc
     3901 gcatcggcgg cgcgcgcgac aggggccagc gcaagcagcc aggcgctcac gaacaggaac
     3961 agcacagcca ggccatgatg gccgatggct tgcgcgcgcc tatttttagt cgccataggc
     4021 tcttcggtcc ccataggctc tttggtcgcc atgaactcca tttgcgtatc ggccgctctc
     4081 gacaggatat ttttctggaa ggcctttgcc atcgcggatc agccttgccg ctgattaacg
     4141 attggtatcc agataaggtt aaccaagcct tttcaacacg cgattacgca gcggatcggc
     4201 tttttgccgg cattggaaag agggttgcct tccaccccgc caaatcataa aagcgatagt
     4261 ggatcactgc aatccttgaa ccgtccttcc atgctacttc ctgtagaagg caaatcgagg
     4321 gacggccatc gcgcaaggcg acataggctg tggatgatcg cgacgaattt cgcagacgtg
     4381 cgcccgtggc gggggaatcg tctgcgtgag gaaaacggca tgggattgtc ccgcgccggc
     4441 tctgtacgag caaacaagct ggtccggatt tccgggcttt ggttcaaaac gttacgctgg
     4501 tgacgatggc tttagacgca attctggaag ggtccgcact gtaccgcgcc attttggcag
     4561 cgggcggcac ctccattgcg ctcagccagc ttcccgcgga cactcaatta tccggcacgc
     4621 gttcaagtcc aggcacatgg cggcgggcat tgccccgcca gctgcggctg gcggctgccg
     4681 ggaggacacg aaataatgcc caacaagatg ctaatagacg cctcccaccc ggaggaaaca
     4741 cgcgttgtcg tggttcgcgg cagccgtatt gaagaattcg acttcgagtc gcaggacaag
     4801 aagcaactca aaggaaacat ctacctcgcc cgcgtaacgc gcgtcgaacc ctcgctccag
     4861 gcagctttcg tcgaatatgg cggcaaccgc cacggcttcc tggccttcag cgaaatccac
     4921 cccgattact atcagattcc ggttgccgac cgtcaggccc tgctgcgcgc cgaggccgag
     4981 gaagccgaag cggaagaaaa cgaggaggcg gacggcgaag gccgtcacga ccgcgatcgc
     5041 ggccgccgtg gccgccgccg tggcggcaag ggccgcgagc gcgccgaaca tcaccgccac
     5101 gacgccgctt cgtccgaagc aggctccgcc gagggtggtg acgaagagcg cgccaatgaa
     5161 aatggcgatg aggccgagga tcgcggcgcg ccggatgcgg ccgaaggcca ggaagctgcc
     5221 gccgtcgaga atcacgacga gcacgacaac gccgccggcg aaccgtccga cggcaagagc
     5281 ggctcgattg ccgccagcgt cgacgccgac gtggtttccg agccggcgcc cgccgaagag
     5341 accggcgaag cgacctccga cgacaatgat cgcggcatgc tggaggaagt ccacgccgcc
     5401 aaccacgacg accacgagat cgaatcggtc ggcgcggaag acgcgctgga tgaggttcgc
     5461 aaccgccgca agccgacgcg ccgccagtac cagatccagg aagtcatcaa gcgccggcag
     5521 atcctgctgg tgcaggtggt caaggaagag cgcggcaaca agggcgcggc catcaccacc
     5581 tatctgtcgc ttgccggccg ctattcggtg ctgatgccca acaccgcgcg cggcggcggc
     5641 atttcgcgca agatcaccaa tgcgcaggac cgcaaacgcc tgaaggaagt ggttgccgac
     5701 ctcgaagtgc cgcagggcat gggcgtcatc ttgcgcaccg ccggcgaaag ccgcaccaag
     5761 gccgagatca agcgcgacta cgaatatctg atgcggctgt gggaaaacgt gcgcaacctc
     5821 acgctgcaat cgaccgcgcc cgccctcgtt tatgaggaag gctcgctgat caagcgttcg
     5881 gtgcgcgacc tctataacaa ggacatcgac gaggttctcg tctccggcga ggacggctac
     5941 cgcgaggcca aggacttcat gcgcatgctg atgcccagcc atgcgaaagt cgtgcagcct
     6001 tatcgggaca cgacgccgat cttcgttcgc tcgggcatcg aggcgcaact cgaccgcatg
     6061 ttgcagccgc aggtaacgct gaaaagcggc ggctacatca tcatcaacca gaccgaggcg
     6121 ctggtcgcca tcgacgtcaa ttcgggccgt tccaccaagg aacactcgat cgaggacacc
     6181 gcgctccaca ccaacctgga agcggcggaa gaagtagctc gccagctgcg gctgcgcgac
     6241 cttgccggcc tgatcgtcat cgacttcatc gacatggagg aaaaccgcaa caaccgcgcg
     6301 gtcgagaagc gcctcaagga tcacttgaag aacgaccgcg cccgcatcca ggtcggccgc
     6361 atctcgcatt tcggcctgat ggaaatgagc cgccagcgta tccgcgccag cgtgctggaa
     6421 tccaccatga agccttgccc gacttgcggc ggcaccggcc atgtgcgctc ggattcgtcg
     6481 gtggcactgg ccgtcgtgcg cgccatcgag gaattcctgc tcaaggattc caagtgccac
     6541 atcaccgtgc gcacgcccgc tgcaacggcg ctctacgtgc tcaaccacaa gcgcggcacg
     6601 ctggtcgaac tggaaagccg cttcggcctg accgtcacca tcgaagccga cgacacggtg
     6661 ggcgcgcagc tttacgcgat ttcgcgtggc ctggtcgccg aaaagcctgc cggcttcgtc
     6721 gagacgcgcg cgctgcccgt ctatgacgag cctgaggaac ccgaaatcgt cgaggaagag
     6781 gacgatgagg catcggagcc ggtggccgag gcaacgccgc gtcagcccga tcaagcggtc
     6841 cggccgacgg aagaaggcga aggccgcgac cgcaagcgcc gccgtaggcg ccgccgtcgc
     6901 ggcggcaagg atcgcgaaaa cggcgacgcc tccaatcagg atgcggcagg cgatgacgcc
     6961 gtgtcggatg aggccggtta cgccgatgtg gaactggatg gcgatgcgcc gctagaagcg
     7021 acggcagcca ccgaagccga tccatcggaa gaagcacagg caaagaaacg tcggcgcggc
     7081 aagcgcggcg gcaagcgcag ccgtcaggac gacgtcgagg ccaatgcgga aggcgaagcc
     7141 gaggcggttg ccgct
//
LOCUS       DASYCS010000399         4893 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_548460,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000399 DASYCS010000000
VERSION     DASYCS010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4893
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548460"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VGM46_12425"
     CDS             <1..292
                     /locus_tag="VGM46_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEY4193444.1"
                     /translation="SEWNNKPVAFVGYGGAGGARAIEQLRQIVVEMQMASTRNAVHIL
                     LPDFLAVLQQGKKLEEIEHLNQSVSPMLDQLSWWAHALKVARDADARTAQAA"
     gene            346..648
                     /locus_tag="VGM46_12430"
                     /pseudo
     CDS             346..648
                     /locus_tag="VGM46_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007635327.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(645..1955)
                     /locus_tag="VGM46_12435"
     CDS             complement(645..1955)
                     /locus_tag="VGM46_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEY4193445.1"
                     /translation="MLYVEIAIVVVLICVNGLLSMSELAIVSSRPARLKAMIDRNVAG
                     AARALELGSDPGKFLSSVQIGITLVGVLSGAFSGATLGGRLSVYLASLGISERFADPA
                     GVGIVVAVITYFSLIAGELVPKQIALRDPEKVAVRVSSAMSMIATVAAPLVFLLDISG
                     RALLWLLGQRGESEEKVTDEEIKMLVAEAEHHGTIESDERRMIAGVMRLGDRAVRAVM
                     TPRTEVDWLNLQSDEATTRKLLMETQHSRLPAADGGVDAMIGVVQTRDLLAAALGGRS
                     FDLRRHVRSAPIVHDQADALDVLAKLKESDVPMALVHDEYGHFEGIVTPADILEAITG
                     VFRADLDAGDSENAVKREDGSWLLAGYMQADEMADTLGIDLPENRDYETVAGYVLSHM
                     HHLPNTGETVDAQGWRFEVVDLDGRRIDKLIATRLASENRDAGR"
     gene            complement(2026..2748)
                     /locus_tag="VGM46_12440"
     CDS             complement(2026..2748)
                     /locus_tag="VGM46_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006338325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LssY C-terminal domain-containing protein"
                     /protein_id="HEY4193446.1"
                     /translation="MGRRSLKRRMAIWTVAFCVAYVGVAYLVAPELWTLHSFLRGPLP
                     AEMVTRTPQGIAGDPINVGLVGTKAEVVEAFGMAKWDTADAVTLRTAIRIGASVLFNR
                     PYPDAPVSPLLFEGRQQDLAFEKPVGNSADQRHHVRLWQTADTGEDGRPLWLGSSSFD
                     RGVGLSHDTGAITHHIGPDIDAERDLVIADLSDAGTLASTNEIEGIGATKAGRNGGGD
                     PYYTDGMALIGLLRVLSPPARE"
     gene            complement(2840..3616)
                     /locus_tag="VGM46_12445"
     CDS             complement(2840..3616)
                     /locus_tag="VGM46_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HEY4193447.1"
                     /translation="MRSRRRPFIVSACAGLAIAAISACTTGSLLTLEPAIDVGVQTAA
                     VPANPGMQALVPSNPYMTAAYPRMDEPAQPSEQMPADQIDCRNELKRLDVAYTDVAPI
                     HDGGQCGIDNPVKVTAIGNIAMKPAATLTCDMAASFASWTKKELVPSARWRYFSGVKA
                     IHQGSSYSCRRIAGEGVLSEHGKGNALDVMSIELTNGDNIDVRKPGLFAFRTRGFLNT
                     VRADGCQYFTTVLGPGYNYDHRNHFHFDIKNRKNGYRACR"
     gene            complement(3720..4718)
                     /locus_tag="VGM46_12450"
     CDS             complement(3720..4718)
                     /locus_tag="VGM46_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006338316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HEY4193448.1"
                     /translation="MGQAATMLHHLPLPDMVSPRLRLAAFLAILFLACPMGLIAATAK
                     SPKLPRRAPVPQLQERPADPPPGVVPVPAAKPGEADFPKPKEAVKPNAAETTLPQLTP
                     ENPGAVPLPPQKPAKATTDAKEPMKPSETKPEKKPIPPDPRSTIIPLNPMPDEEVACR
                     TRLKALGVEFEEHEAERDAEIGCSIPYPIVLKTLGGGINLEPKAELNCTMAQAAARFM
                     STSVAPAVKSQFNADVKTVSQASAYVCRPRHNGRKMSEHAFGNALDIASFTLTDGTKV
                     DIEPVPPQKNASFLSKIRKAACGPFKTVLGPGSDPDHSLHFHIDLEPRRNGGTFCQ"
BASE COUNT          893 a         1471 c         1603 g          926 t
ORIGIN      
        1 tagcgaatgg aacaacaagc cggtggcctt tgtcggctat ggcggcgccg gcggggcgcg
       61 cgccatcgag cagcttcgcc agatcgtcgt cgagatgcag atggcgtcga cgcgcaacgc
      121 cgttcacatc ctgctgcccg acttcctcgc cgtgctccag cagggcaaga agcttgagga
      181 gatcgaacat ttgaaccaga gcgtcagccc catgctcgac cagctttcct ggtgggcgca
      241 tgcgctgaag gtggcgcgcg acgcggatgc gcgcacggcg caggccgcct gagtatcgat
      301 tttggcagaa tgaagaaggg cggcaatctt gccgcccgga ttgcccgtca cgccttcgaa
      361 tacgcttgcg ccaccaacga catcgggcac cggaccgcca aggccaggca tccatggacc
      421 aacgatcagg tcagcggatg aaccggacca tcaaggatgc caccgtcaag cgcttccacc
      481 atgagggcca tgaccagcta tctcggcacc tcgctgactt cgtctcagcc tacgacgtcg
      541 ctcgcaggct gaagacactc aaggagtctt actcccaatg aatttgtctg tagccaaaac
      601 agctcgaacg cttcactgtt aacccgctcg ccgagactaa acagctagcg tccggcgtcg
      661 cggttttcgc tggccagcct cgtggcgatc agtttgtcga tgcggcggcc atcgagatcg
      721 acgacttcga agcgccagcc ctgcgcgtcg acggtttcac cggtgttggg caaatggtgc
      781 atatgcgaaa gcacgtagcc ggcgaccgtt tcgtagtcgc ggttttccgg caggtcgatg
      841 cccagcgtgt cggccatctc gtcggcctgc atgtagccag cgagcagcca tgagccgtct
      901 tcgcgcttga cggcgttttc ggaatcgccg gcatcgagat cggcgcgaaa cacgccggta
      961 atcgcttcca gaatatcggc tggagtgacg atgccttcga aatggccata ctcgtcatgc
     1021 accagcgcca tcggcacatc gctttccttg agcttggcca gcacgtcgag cgcgtcggcc
     1081 tggtcatgga cgatcggggc ggagcgcaca tgccggcgca ggtcgaagct gcggccgcca
     1141 agggccgcgg caagcaggtc gcgggtctgg acgacaccga tcatcgcatc gacgccgcca
     1201 tcggcggccg gaaggcgcga gtgctgggtt tccatcagaa gcttgcgggt agtggcttcg
     1261 tccgactgga gattgagcca gtcgacctca gtgcgcggcg tcatcaccgc gcgcaccgca
     1321 cggtcgccaa gccgcatgac gccggcgatc atgcggcgct cgtcggattc gatggtgcca
     1381 tgatgctcgg cttcggcgac cagcatcttg atttcctcgt cggtgacctt ttcctcgctc
     1441 tcgccacgct ggccaagcag ccacagcagc gcgcggccgg aaatgtcgag caggaagacg
     1501 agcggcgcgg ccaccgtcgc gatcatgctc atggcggatg atacgcgcac ggccaccttt
     1561 tcggggtcgc gcaacgcgat ctgcttgggc accagttcgc cggcgatcag cgagaaataa
     1621 gtgatgacag cgacgacgat gccgacgcct gcggggtcgg cgaagcgttc gctgataccg
     1681 agcgaggcga ggtagacgga cagccgcccg cccagcgtcg caccggaaaa ggcgcccgaa
     1741 agcacgccga ccagcgtgat gccgatctgg accgaagaca ggaatttgcc cggatcggag
     1801 ccaagttcca acgcgcgggc ggcgccggcc acattacggt cgatcatcgc cttcagcctt
     1861 gcaggccgcg acgagacgat ggcaagttcc gacatcgaca gcaggccgtt cacgcagatg
     1921 aggacgacca caatggcaat ttcgacataa agcatggccg ctcaatagca ttacgcaagc
     1981 ttgttcggaa ggagccaggc ttgccgcaaa tccatttgcc gcccgctatt cccgcgcggg
     2041 tggcgaaagc acccgcagca ggccgatcaa ggccatgccg tcggtgtagt aggggtcgcc
     2101 gccaccgttg cggccggcct tggtcgcgcc gatgccttcg atctcattgg tggaggccag
     2161 cgtgccggca tccgacaggt cggcgatcac caggtcgcgc tcggcatcga tatcggggcc
     2221 gatgtggtgg gtgatggcgc cggtgtcgtg gctgaggccg acgccgcggt cgaagctcga
     2281 cgaacccagc cagagcgggc ggccgtcctc gccggtatcg gcggtctgcc acaggcgcac
     2341 atggtggcgc tggtcggcgc tgttgccgac cggcttttcg aaggcaagat cctgttgccg
     2401 gccttcgaac agcagcgggc tgacgggtgc gtcggggtag ggccggttga acaggacgct
     2461 ggcgccgatc cttattgcgg ttctcagcgt aacggcgtcg gcggtgtccc atttggccat
     2521 gccgaaggcc tcgacgacct cggccttggt gccaaccagc ccgacattga tgggatcgcc
     2581 ggcgatgcct tgcggcgtgc gcgtcaccat ctcggcaggc agcggaccgc gcagaaagct
     2641 gtgcagcgtc cacagttcgg gcgcgaccag ataggcgacg ccgacatagg caacgcaaaa
     2701 cgccaccgtc cagatcgcca ttcgccgttt gagacttctt cggcccatga ggatgatctt
     2761 atcggaaaac tacgttcgtt cgcaccatgg ggtcgagcac gatcttatca aagccgtttc
     2821 gcacttttcg gggccatgcc tagcggcagg cgcggtagcc gttcttgcgg ttcttgatgt
     2881 cgaaatggaa atggttgcgg tggtcgtaat tgtagccggg gccaagcacg gtagtgaaat
     2941 actggcagcc atcggcgcgc accgtattga ggaagccgcg cgtgcgaaag gcgaaaaggc
     3001 cgggcttgcg cacgtcgata ttgtcgccat tggtcaattc gatgctcatg acatcgagcg
     3061 cgttgccctt gccatgctcg gacagcacgc cttcgccggc gatccggcgg caggaatagc
     3121 tggaaccctg atggatcgcc ttgacgccgg agaaatatcg ccagcgcgcc gaaggcacca
     3181 gctccttctt cgtccaactc gcgaagctgg cggccatgtc gcaggtgagc gtcgccgccg
     3241 gcttcatggc gatgttgccg atggccgtca ctttgaccgg attgtcgatg ccgcattggc
     3301 ctccatcatg gatcggcgca acatcggtat aggcgacgtc gagccgcttc agttcgttgc
     3361 gacaatcgat ctggtcggcg ggcatttgtt cggatggttg cgccggctcg tccatgcgcg
     3421 ggtaggcggc ggtcatataa gggttggaag gcaccagggc ttgcatgccg ggattggccg
     3481 gcacggcagc ggtctggacg ccgacatcga tggccggttc cagcgtcagc aggctgccgg
     3541 tggtgcaggc ggaaatcgcc gcaatggcga gacccgcgca cgcgctgacg atgaacggcc
     3601 tgcgccgcga ccgcatattt cccatttccc gtttgtgttc ctgtcccatg atggaacaga
     3661 gtacctttca agggtaaacg aaaaatcagt gcggcattca tggtcgaggc gggcaacgct
     3721 cattggcaaa aggtgccgcc gttgcggcgc ggctccaagt cgatatggaa atgcagggaa
     3781 tggtccggat cgctgcccgg ccccagcacg gtcttgaacg ggccacaggc cgccttgcgt
     3841 attttcgata aaaaactggc gttcttttgc ggcggaacag gctcgatatc gaccttcgtg
     3901 ccatcggtca gggtaaagct tgcgatgtcg agcgcgttgc cgaaggcatg ttcggacatt
     3961 ttccggccat tgtggcgcgg gcggcaaaca taggccgatg cctggctgac ggttttcaca
     4021 tcagcattaa actggctttt caccgccggg gccacgctgg tcgacatgaa ccgggcggcg
     4081 gcttgcgcca tggtgcaatt cagctcggcc ttcggttcga gatttatgcc gccgccgagt
     4141 gtcttcagca cgatcgggta ggggatggaa cagccgatct cggcatcgcg ttcggcctcg
     4201 tgctcctcga actcgacacc gagcgctttc aaccgcgttc ggcaggcgac ttcctcgtcc
     4261 ggcatgggat tgaggggaat gatcgtggag cgagggtccg gcgggatcgg cttcttttcc
     4321 ggtttcgtct cggaaggttt catcggctcc ttggcgtcgg tggtggcctt ggctggtttt
     4381 tgcggcggca gggggacagc gccggggttt tcaggcgtca gttgcgggag cgtggtttcc
     4441 gcagcgttcg gctttacagc ctcctttggc ttcgggaaat cggcttcgcc aggcttcgcc
     4501 gccggcacgg gaacgacgcc cggtggcggg tccgctggcc tttcttgcag ttgcggcacc
     4561 ggcgcccggc gcggaagctt cggcgatttt gccgtcgcgg cgatcagacc catcggacag
     4621 gccaggaaca ggatagcgag gaaggctgct agacgaaggc gcggtgaaac catgtccggc
     4681 aacggcaagt gatgcagcat ggttgcggcc tggcccatca cattcggtga ccgttttgtc
     4741 gcggaggccg gccggcgcaa ataaaatcag tggcgacagc gcgctgacat actgagtcgg
     4801 gagttcgcct caagctgttg cgttgaaagg attatctgcc tcaggcgttg cggtcggcct
     4861 taggttcgaa cgaaccgtcc agggcatcag gcg
//
LOCUS       DASYCS010000400         5210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1564154,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000400 DASYCS010000000
VERSION     DASYCS010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 5210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5210
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1564154"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /gene="nuoF"
                     /locus_tag="VGM46_12455"
     CDS             <1..740
                     /gene="nuoF"
                     /locus_tag="VGM46_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEY4193449.1"
                     /translation="RLKPPFPANVGLYGCPTTVNNVESIAVAPTILRRGAAWFSSFGR
                     PNNVGTKLFCVSGHVNNPCTVEEAMSIPFRELIETHCGGIRGGWDNLLAVIPGGASVP
                     LVPAEQIIDTPMDFDALRDLKSGLGTAAVIVMDKSTDIVKAIARLSYFYKHESCGQCT
                     PCREGTGWMWRVMERLVVGQAQKREIDMLLDVTKQVEGHTICALGDAAAWPIQGLMRH
                     FRGEVERRIDEFSRNAHQVEPMMVAAE"
     gene            737..1366
                     /locus_tag="VGM46_12460"
     CDS             737..1366
                     /locus_tag="VGM46_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone dehydrogenase"
                     /protein_id="HEY4193450.1"
                     /translation="MTMVDDPKNPQPMEMAGAIDLLAHPVAGVAALSAFGFGLASHAF
                     GFWMGTLTGMASASQLFLDQQNAAASPHAPAKQAEASRKATAEIKQFPTGGVASKRRT
                     AARAGTGVRKSPASVQAPALAREVSAGDHRGGDDLKAIAGVGPKLEQVLNGLGIHSYR
                     QVAALTGEEIVTLEQKLGLQGRIERDGWTAQAMTLAGSSGAGSSGGDDR"
     gene            1366..1635
                     /locus_tag="VGM46_12465"
     CDS             1366..1635
                     /locus_tag="VGM46_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193451.1"
                     /translation="MDVHGKRDVFDVREALAANAGFSDADLSAAVFQLGNGRIGDADL
                     SGAMPSNVNLSDVKIEKAIDAGMKFDGVAVSDLSVAYEAAKAGGK"
     gene            1635..3716
                     /gene="nuoG"
                     /locus_tag="VGM46_12470"
     CDS             1635..3716
                     /gene="nuoG"
                     /locus_tag="VGM46_12470"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018447543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HEY4193452.1"
                     /translation="MAKLKVDGKEIEVPDHYTLLQAAEEAGAEVPRFCFHERLSIAGN
                     CRMCLVEVKGGPPKPQASCAMGVRDLRPGPNGEPPEVFTNTPMVKKAREGVMEFLLIN
                     HPLDCPICDQGGECDLQDQAMAFGVDSSRFHENKRAVEDKYIGPLVKTIMNRCIHCTR
                     CVRFTTEVAGISELGLIGRGEDAEITTYLESAMTSELQGNVIDLCPVGALTSKPYAFQ
                     ARPWELTKTESIDVMDAVGSAIRVDSRGREVMRVMPRINEQVNEEWISDKTRFIWDGL
                     RTQRLDRPYVRKDGKLAPASWAEAFSAIKDAVSKTSPDRIGAIAGDLAAVEEMYALKL
                     LMASLGSANIDCRQDGAALDPALGRSSYIFNPTIEGIEQADALLIIGANPRFEASVLN
                     ARIRKRFRANSLPVGVIGDIGDTRYDYELLGAGADTLKDLADGNGKFFQALKKAKHPL
                     IIVGQGALSRADGGAVLGQAARLAAAVDASRADWNGFAVLHTAAARVGGLDLGLVPGE
                     GGKNVGAMLGGTDVLFLLGADELDMAQTAGSFVVYIGTHGDAGAHRADVILPGAAYTE
                     KSGTYVNTEGRVQQTSRAGFAPGDAREDWAIFRALSDVLGKKLPFDSLPQLRARLYGE
                     FPHFARIDQIAAGEAGDIAKVAKLGGRLDKGAFMSPVQDFYLTNPIARASAVMAECSA
                     LARNGFKQAAE"
     gene            3720..4763
                     /gene="nuoH"
                     /locus_tag="VGM46_12475"
     CDS             3720..4763
                     /gene="nuoH"
                     /locus_tag="VGM46_12475"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004683616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEY4193453.1"
                     /translation="MDTFFSFYVLPALIILLKSVVLIVILLIGVAYILYADRKIWAAV
                     QLRRGPNVVGPWGTLQAFADLLKFVFKEPIIPSGANKGVFLLAPLVSAVLAISAWAVI
                     PVTSGWAIANINVGILYVFAISSLEVYGVIMGGWASNSKYPFLGALRSAAQMVSYEVS
                     IGFVIVTVLLCVGSLNLTDIVMSQQNGLGTRLGLPNTLLDWHWLSLFPMFVIFFISAL
                     AETNRPPFDMVEAESELVAGHMVEYSSTPFLLFFLGEYVAVVLMCALTTILFLGGWLP
                     PFDFVPFTWVPGVIWFVLKLCLVFFMFSMVKAIVPRFRYDQLMRLGWKVFLPISLFMV
                     VATAAFLKITGFA"
     gene            4763..>5210
                     /gene="nuoI"
                     /locus_tag="VGM46_12480"
     CDS             4763..>5210
                     /gene="nuoI"
                     /locus_tag="VGM46_12480"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEY4193454.1"
                     /translation="MSALSQAAKSLLLQDFVSAFFLGMRQFFAPKETINYPHEKGQTS
                     PRFRGEHALRRYPNGEERCIACKLCEAICPAQAITIEAGPRRNDGTRRTVRYDIDMVK
                     CIYCGFCQEACPVDAIVEGPNFEFATETREELYYDKDKLLANGDRWE"
BASE COUNT          932 a         1520 c         1693 g         1065 t
ORIGIN      
        1 cccggctgaa gccgcctttc ccggccaatg tcggcctcta tggctgcccg acgacggtga
       61 acaatgtcga atcgatcgct gtcgcgccga ctatcctgcg gcgcggcgcg gcgtggtttt
      121 cgtcatttgg ccgcccgaac aatgtcggca ccaagctttt ctgcgtttcc ggtcacgtca
      181 acaatccgtg tacggtcgaa gaagcgatgt cgatcccgtt tcgcgagctg atcgagacgc
      241 attgcggcgg cattcgcggc gggtgggaca atctgctggc cgtcattcca ggcggcgcat
      301 ctgtgccgct ggtgccggcc gaacagatca tcgatacgcc gatggatttc gatgcgctgc
      361 gcgacctgaa gtcaggcctc ggcacggcgg cggtcatcgt gatggacaag tccaccgata
      421 tcgtgaaggc gattgcgcgg ctttcctatt tctacaagca tgaaagctgt ggccagtgca
      481 cgccttgccg cgaaggcaca ggctggatgt ggcgggtgat ggaacgcctc gtcgtcggcc
      541 aggcgcagaa gcgcgaaatc gacatgctgc tcgatgtcac caagcaggtg gagggccaca
      601 cgatctgcgc gctgggcgat gcggctgcct ggccgatcca aggcctcatg cggcatttcc
      661 ggggtgaagt agagcggcgc atcgacgaat tctcgcgcaa cgctcatcag gtcgagccaa
      721 tgatggtggc agcagagtga cgatggtcga cgatccgaaa aacccgcagc cgatggagat
      781 ggcaggcgcc atcgatctgt tggcgcaccc cgttgccggg gtcgcggcgc tatcggcatt
      841 cggcttcggg ctggccagcc atgctttcgg tttctggatg ggtacgctta ccggtatggc
      901 cagcgcctcg cagctttttc tcgaccagca aaatgccgcc gcttcgccgc atgcccccgc
      961 gaaacaggcg gaggcttcaa gaaaggcgac agccgagatc aagcagtttc ccaccggcgg
     1021 cgtcgcctcg aaaaggcgca cggctgcgag ggccggaacg ggcgtgagaa aatcgccggc
     1081 atcggtgcag gcaccggcat tggcgcgcga ggtttcggcg ggcgatcatc gtggtggcga
     1141 cgatttgaag gcgattgccg gcgtggggcc gaagctggag caggttttga acgggcttgg
     1201 cattcattcc tacaggcagg ttgccgcctt gaccggcgaa gagatcgtca cgctcgagca
     1261 aaagctcggc ttgcaagggc ggatcgagcg cgacgggtgg actgcccagg ccatgacgtt
     1321 ggctggttca tctggagccg gatcatccgg tggagatgac cgctgatgga tgttcatggg
     1381 aaacgcgatg tgtttgacgt gcgcgaggca ttggccgcaa acgcagggtt cagcgacgcc
     1441 gatctgtcgg cagccgtttt tcaactgggc aatggccgga tcggtgatgc agacctgtcc
     1501 ggcgcgatgc cgtcaaatgt aaatttgagc gacgtgaaga tcgaaaaggc aatcgacgcc
     1561 ggcatgaaat ttgacggcgt ggcagtcagc gatctttccg tagcgtatga agcggcgaaa
     1621 gccgggggca aataatggca aagctcaagg tcgacgggaa agagatcgag gtacccgacc
     1681 actacacgct gttgcaggcg gcggaagagg cgggcgcgga agtgccgcgt ttctgcttcc
     1741 atgaacggct gtcgattgcc ggcaactgcc gcatgtgcct ggttgaggtg aagggcggcc
     1801 cacccaagcc gcaggcctca tgtgccatgg gcgtgcgcga tctgcgcccc ggcccgaacg
     1861 gcgagccgcc ggaagtgttc accaacacgc cgatggtcaa gaaggcgcgc gaaggcgtga
     1921 tggagttcct gctcatcaac catccgctcg attgcccgat ttgcgatcag ggcggcgaat
     1981 gcgacctgca agaccaggcg atggcgttcg gcgtcgattc ctcgcgcttc catgagaaca
     2041 agcgcgcggt cgaagacaaa tatatcggcc cgctggtcaa gacgatcatg aaccggtgca
     2101 tccattgcac gcgttgcgtt cgcttcacca cggaagtggc cggcatttcc gagcttggcc
     2161 tgatcggccg cggcgaggat gccgaaatca ccacctatct cgaaagcgcg atgacctccg
     2221 aattgcaggg caatgtcatc gacctttgcc cggtcggcgc gctgacctcg aagccttatg
     2281 cgttccaggc gcgtccgtgg gaactgacca agaccgaatc catcgacgtg atggacgcgg
     2341 tcggctcggc catccgcgtc gacagccgtg ggcgcgaagt gatgcgcgtc atgccgcgca
     2401 tcaacgagca ggtgaacgag gagtggattt ccgacaagac ccgcttcatc tgggacggcc
     2461 tgcgtacgca gcgcctcgac cggccttatg tgcgcaagga cggcaagctc gcgcccgcca
     2521 gctgggccga agcattttcg gccatcaagg acgcggtttc gaagacttct ccagacagga
     2581 tcggcgcgat tgccggcgat ctggcggctg tcgaggaaat gtatgcgctg aagcttctga
     2641 tggcttcgct cgggtcggcc aatatcgatt gccggcagga tggcgcagca ctcgatccag
     2701 cgcttggccg gtcgagctat attttcaatc ctaccatcga aggcatcgaa caggcggacg
     2761 cgctcctcat tatcggggcc aatccgcgct ttgaagcgtc ggttctcaac gcccgcattc
     2821 gcaagcgttt ccgcgccaac tcgctgccgg tcggcgtgat cggcgatatt ggcgacaccc
     2881 gatacgacta cgaattgctg ggcgccggcg ccgatacgtt gaaggatctg gcggacggca
     2941 acggcaagtt cttccaggcg ctgaagaagg ccaagcatcc gcttatcatc gtcgggcagg
     3001 gcgctttgtc gcgcgccgat ggcggtgcgg tgctgggaca agcggccagg cttgcggctg
     3061 ccgtcgatgc cagccgcgcg gactggaacg gttttgccgt gctgcatacg gctgccgccc
     3121 gtgtcggcgg cctcgacctc ggcctggtgc cgggcgaagg cggcaagaat gtcggcgcga
     3181 tgctgggcgg cacggacgtg ctgttcctgc tcggggccga tgaactggac atggcgcaga
     3241 ccgccggctc gtttgtcgtt tatatcggca cgcatggcga tgccggcgcc catcgcgccg
     3301 atgtcatctt gccgggcgcg gcctatacgg aaaagtcagg cacctacgtc aatacggaag
     3361 gccgcgtgca gcagaccagc cgcgccggtt tcgcgccggg agacgcgcgg gaagactggg
     3421 cgattttccg tgcgctgtcg gatgtgctgg gcaagaaact gccgtttgat tcgctgccgc
     3481 agcttcgcgc caggctctac ggcgagtttc cgcattttgc ccggatcgac cagatcgccg
     3541 ccggcgaggc gggcgatatt gcgaaagtgg cgaagctcgg tggacggctg gacaagggcg
     3601 ctttcatgtc gccggtccag gatttctacc tgaccaaccc gattgcgcgc gcttcggctg
     3661 tgatggcgga gtgctcggcg ctggcccgga acggtttcaa gcaggcggcg gaatagagca
     3721 tggacacctt cttttccttc tacgttctgc cggcgctgat catcctgctg aaatcggtcg
     3781 tgctgatcgt catccttttg atcggggtgg cctatattct ttatgccgac cgcaagatct
     3841 gggcggcagt gcagctgcgc cgtggcccca acgtcgtcgg tccatgggga acgctgcaag
     3901 ctttcgccga cctgttgaag ttcgtcttca aggagccgat cattccatcg ggcgccaaca
     3961 agggcgtgtt cctgctggcg ccgctggttt cggccgtact ggcgatttcg gcgtgggcgg
     4021 tgattccggt tacttccggc tgggccatcg ccaacatcaa tgtcggcatc ctctacgtgt
     4081 tcgccatttc ctcgctagag gtctacggcg tcatcatggg cggctgggca tccaattcga
     4141 aatatccgtt cctcggcgcg ctgcgctcgg cggcgcaaat ggtgtcttac gaagtctcga
     4201 tcggcttcgt catcgtcact gttttgctgt gcgtaggttc gctgaacctg acggatatcg
     4261 tgatgtcgca gcaaaacggg ttgggcacgc gccttggcct gcccaatacg ctgcttgatt
     4321 ggcactggct gtcgctgttt ccgatgttcg tgatcttttt catctcggca ctggccgaaa
     4381 cgaaccgtcc gcccttcgac atggtggagg ccgaatcgga actggtcgcc ggccatatgg
     4441 tcgaatattc atcgacgccg ttcttgctgt ttttcctcgg cgaatatgtc gcggtcgtct
     4501 tgatgtgcgc gctcaccacc atcctgttcc tcggcggctg gctgcctccc ttcgatttcg
     4561 tgccgttcac ctgggtgccg ggcgttatct ggttcgtgct caagctttgc ctggtgttct
     4621 tcatgttctc catggtcaag gcgatcgtgc cgcgcttccg ctacgaccaa ctgatgcggc
     4681 tgggctggaa ggtgtttctg ccgatttcgc tgttcatggt ggtggcgacg gccgccttcc
     4741 tcaaaatcac gggatttgcc tgatgtcggc cttgtctcaa gcggccaagt cgctgttgtt
     4801 gcaggatttc gtttcggcgt ttttcctggg gatgcgccag tttttcgcgc cgaaggaaac
     4861 gatcaactat ccgcatgaaa aagggcagac gagtccgcgt tttcgtggcg aacatgcgct
     4921 gcgccgctat ccgaacggcg aggagcgctg cattgcctgc aagctgtgcg aggcgatctg
     4981 cccggcgcag gccatcacca tcgaggcggg gccacgccgc aatgacggaa cgcgccgcac
     5041 cgtgcgctac gatatcgaca tggtgaagtg catctattgc ggcttttgcc aggaagcctg
     5101 cccggtcgac gccatcgtcg aagggccgaa tttcgaattc gcgacggaaa cgcgcgagga
     5161 actttactac gacaaggaca agcttctggc gaatggcgac cgctgggagc
//
LOCUS       DASYCS010000401         3410 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4464218,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000401 DASYCS010000000
VERSION     DASYCS010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4464218"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VGM46_12485"
     CDS             <1..286
                     /locus_tag="VGM46_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HEY4193455.1"
                     /translation="AFTAFTDGRSYSKAQLLRSRHGFAGAVRAVGQVLVDQLPHMLRV
                     GFDEFEITNPVLLKRLEAGDIGGLPLYYQPSAKPVEAGEKYSWRRQPAGD"
     gene            complement(406..1422)
                     /locus_tag="VGM46_12490"
     CDS             complement(406..1422)
                     /locus_tag="VGM46_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEY4193456.1"
                     /translation="MNIISPEEASRRAWLKILSRYKKPNRKRSTIELGVTLVPFIALW
                     ALCSISYVYGYWWGLFLIVPAAGFLVRMFMIQHDCGHGSFFANRHADDWIGRALGVFT
                     MTPYDCWRRAHATHHASAGNLDERGTGDIKTLTVSEYKNLPLHRRLAYRLYRHPLVMF
                     GIGPTWIFVFEQRLPVGMMRGGVTPWVSSMATNLGIALAAAILIWFIGFVAFLVIHLS
                     IVILAGSVGIWLFYVQHQFEETEWARDEKWEFQYAALHGSSYYDLPAVLRWFTGNIGI
                     HHVHHLSAKVPHYRLPEVLRDYPELRNIGRITLLDSLRCVKLALWDENRGKLVSFREA
                     RAMA"
     gene            complement(1475..1702)
                     /locus_tag="VGM46_12495"
     CDS             complement(1475..1702)
                     /locus_tag="VGM46_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006200407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193457.1"
                     /translation="MTAKNHRSGQEKLAADIKRDIGSGNTARLLHASPAFKPAADLPD
                     YLKHLLDQLKEVRPPKANARVEKEHTRHYAR"
     gene            1949..2584
                     /gene="grpE"
                     /locus_tag="VGM46_12500"
     CDS             1949..2584
                     /gene="grpE"
                     /locus_tag="VGM46_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEY4193458.1"
                     /translation="MSDHANNEHAPDDIEAENMSTERSEGGVDGDYEALVRLLKENEE
                     LKDRALRTAAEMENLRRRTARDVVDARTYAVANFARDMLQVADNLQRATDAIPPESKA
                     SGDAGFKALIDGVELTARSMLTTLERHGVKQLTPEGERFDPNFHQAMFEVPNPDIANG
                     TVVQVVQPGYSIGDRVLRPAMVGVSKGGPKFAAGETPAEPGPVNGQAEKDA"
     gene            2695..3327
                     /locus_tag="VGM46_12505"
     CDS             2695..3327
                     /locus_tag="VGM46_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HEY4193459.1"
                     /translation="MNLIELLDYAGVVVFAATGALAASRKQLDIIGFLFLACVTGIGG
                     GTFRDLILGVPVFWVGNPVYLLICVLVAGAVFFTAHRVESRYRLLLWLDAVGLAAFCV
                     MGAAKGLAITGSPTVAIVTGVLTATFGGILRDLLAGEPSVLLKPEIYVTAALAGACLF
                     TLGDLAGLTPLLSDAIGFAAAFFVRGGALKFGWAFAPYKSRPGRDPDDIT"
BASE COUNT          658 a          996 c         1066 g          690 t
ORIGIN      
        1 ggccttcacc gccttcaccg atgggcgcag ctattccaag gcgcagctgc tgcgcagccg
       61 gcatggcttt gcgggcgcgg tgcgcgctgt cgggcaggtt ctggtcgacc agctgccgca
      121 tatgttgcgg gtcggcttcg atgagtttga aattaccaac ccggttttgc tgaaacgtct
      181 cgaagccggc gatattggcg gcctgccgct ttattaccag ccatctgcta aaccggtgga
      241 agcaggcgag aaatattcct ggcgccgcca acccgccggc gactgaagcc ccgccgcgaa
      301 accttgagca ttccagcaaa agtgtgaaac ggttttgcgt ccggaagtgc ggccaaaaca
      361 aagatatgga gcggttttgt cgattcagta aaaatcggaa ccgctctacg ccatcgccct
      421 cgcttcgcgg aaggaaacca gcttgccgcg gttttcgtcc cacagcgcca gtttcacgca
      481 gcgcaggctg tcgagcagcg ttatgcggcc gatattgcgc agttccggat agtcgcgaag
      541 cacttccggc agccggtagt gcggcacctt ggccgacaga tggtggacat ggtggatgcc
      601 gatattgccg gtgaaccagc gaagcaccgc gggaagatcg tagtaggacg agccgtgcag
      661 ggcggcatac tggaattccc atttctcatc gcgcgcccac tctgtctcct cgaattgatg
      721 ctggacataa aacagccaga tgccgactga gccggcgagg atgacgatcg acaggtggat
      781 gacgaggaat gcgacgaagc cgatgaacca gatgaggatc gcggcggcga gcgcgatgcc
      841 cagattggtg gccatggacg acacccacgg cgtcacgccg ccccgcatca tcccgaccgg
      901 cagcctttgt tcgaaaacga aaatccaggt tggccctatg ccgaacatca ccaaagggtg
      961 gcgatagagc cggtaggcga ggcggcggtg cagcggcagg ttcttgtatt cgctgacggt
     1021 cagcgtcttg atgtcgccgg tgccgcgctc gtccaggttt ccagcgctgg catgatgggt
     1081 tgcatgcgcg cggcgccagc aatcatatgg cgtcatcgta aagacgccca aagcccggcc
     1141 gatccaatca tcggcatggc ggttggcgaa gaacgagccg tgcccgcaat cgtgctggat
     1201 catgaacatg cgcacgagga aacccgccgc cggcacgatg aggaacaggc cccaccaata
     1261 accatagaca tatgaaatgg agcacagcgc ccacagggcg atgaagggaa ctagcgtgac
     1321 gccaagctcg atcgtgctgc gctttcggtt cggcttcttg tagcgcgaca gtatttttag
     1381 ccaggcgcgc ctgctggctt cttccgggga aataatattc atcggcaagc ataggcaggc
     1441 tggccggagg ctgcaagatg gagttcacga aagtttaccg cgcgtagtgt cgcgtgtgtt
     1501 ccttttcgac tcgggcgttg gccttcggcg ggcgaacttc tttcaactgg tccagcaggt
     1561 gcttcagata gtcgggcaga tcggccgcgg gcttgaaggc cggtgatgcg tggagaagcc
     1621 gcgccgtatt gccgctgccg atgtcgcgct tgatgtcggc ggcgagtttt tcctgtccag
     1681 atctgtggtt tttggctgtc atttcaaaat cctttgtcga cttcgaaatc ccccagtagc
     1741 tgtcaggttc ccataatgtg atcagctggc aagcgatgag caaaattacg gtaaaatttg
     1801 aattaacttt gaacgttgtt cacggcctgc gcgttgaccg cagggatcgt ctgaatcggc
     1861 cttgcccctt gatttttgac cgccgaacct cgatatcggc aacaagttcg aaacaaacga
     1921 aataacgggc aagttaacgg gaatggcgat gagcgaccac gcgaacaacg aacatgcgcc
     1981 cgacgacatc gaagccgaaa acatgtcgac ggaacggtcg gaaggcggcg tcgacggcga
     2041 ttatgaggct ttggtgcgtc tcctcaagga aaacgaggag ctgaaggacc gcgcgctgcg
     2101 tactgcggcc gaaatggaaa atctgcgccg ccgcaccgcg cgtgacgtgg tcgatgcgcg
     2161 cacctatgcg gtagcgaatt ttgcccgcga catgttgcag gtggccgaca atcttcaacg
     2221 cgcgacggat gccatcccgc ctgaaagcaa ggcgtcgggc gacgcggggt tcaaggcgct
     2281 gatcgatggc gtcgagctga ccgcgcgcag catgctgacg acgctggagc ggcacggcgt
     2341 caagcaactg acaccggaag gcgaacgctt cgaccctaat ttccaccagg cgatgttcga
     2401 ggttcccaac cccgatatcg ccaatggcac cgtcgtgcag gtggtccagc cgggctattc
     2461 gatcggcgac cgcgtcctgc gcccggccat ggtcggcgtt tccaagggcg gacccaaatt
     2521 cgccgccggc gaaaccccgg cggaacccgg tccggtcaac gggcaggcgg agaaggacgc
     2581 ctagggaaaa cagcggccag ccggccaagc gcggccaacg aggccgcgcg gctttgcatc
     2641 ggcgcgttgc cggtgtaggc cttgcgggta tttatcgctt ttccggcggc cttgatgaac
     2701 ctgatcgagc ttctcgacta tgccggcgtc gtggtcttcg cggccaccgg cgcgctggcg
     2761 gcgtcgcgca agcaactcga cattatcggt ttcctattcc tggcctgcgt taccggtatc
     2821 ggcggcggca cgtttcgcga tcttatcctc ggcgtgccgg ttttctgggt ggggaatccc
     2881 gtctatctgc tgatttgcgt gcttgtagcg ggggcggttt tctttaccgc gcaccgggtc
     2941 gaatcccgct acaggctgct gctctggctc gatgccgtcg gccttgccgc cttctgcgtc
     3001 atgggcgcgg cgaagggcct ggcgatcacc ggttccccga ccgttgccat cgtcaccggc
     3061 gtgctgacgg cgacctttgg cggaatcctg cgcgatctgc tggccggcga gccatccgtc
     3121 ctgctgaaac cggaaatcta tgtgacggcc gcccttgccg gtgcctgcct gttcacgctt
     3181 ggcgacctgg ccggcctgac gccgctgctt tcggatgcaa tcggatttgc ggcggcattt
     3241 ttcgtgcgcg gcggcgcgct taaattcggc tgggcttttg cgccgtataa aagccgcccc
     3301 ggccgcgatc ccgacgatat tacgtgacgg ctttctagag cgattccgat ttttgctgaa
     3361 tcgacaaaac cgctctatct ctttgttttg gccgcatttc cgggcgcaaa
//
LOCUS       DASYCS010000402         4055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_5189461,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000402 DASYCS010000000
VERSION     DASYCS010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5189461"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VGM46_12510"
     CDS             complement(<1..333)
                     /locus_tag="VGM46_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY4193460.1"
                     /translation="MKFTWDSAFALLIITGTLLGVSLPLGRMATNAGVAGIVWAFVIS
                     LGAGGILLCVHLLRGNRIRLTPHKLRYFVVVAAVSYAIPNLLIFSAISHLGAGYTGIM
                     FTLSPIVTL"
     gene            complement(423..848)
                     /locus_tag="VGM46_12515"
     CDS             complement(423..848)
                     /locus_tag="VGM46_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006328568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HEY4193461.1"
                     /translation="MIRFSLTCENAHEFEGWFRSNEDFDKQALRGLVDCPSCGSHKVE
                     KALMAPAVSTGRKQEKIALAMGEAQKQAMAQMKAMAEKIRENADYVGDKFAEEARKIH
                     FGEADARGIYGEATFDEAKSLAEDGVAFMPVPVFPDDRN"
     gene            complement(845..1714)
                     /locus_tag="VGM46_12520"
     CDS             complement(845..1714)
                     /locus_tag="VGM46_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018429541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEY4193462.1"
                     /translation="MGVFKVAAVQMRSGTDPQRNVVEMERLVREAAGQGATYVQTPEM
                     TGAMVRAANTRKAIFAVEANDKVVAAARRLAGELGIFLHIGSTAILRADGKVANRAYL
                     FSPQGGLVAGYDKIHMFDVDLDNGESWRESAVYEPGAQAVVADLNGTKLGFSVCYDLR
                     FPQLFRAEALAGAEILTVPAAFTRQTGEAHWHVLLRARAIENGAFVVAAAQGGLHEDG
                     RETYGHSLIVDPWGRVLAEVDHDEPGVIVAEIDPAQSQAARRKIPNLKNAREFALATE
                     SGAGVGTLRGAAS"
     gene            complement(1729..1995)
                     /gene="grxC"
                     /locus_tag="VGM46_12525"
     CDS             complement(1729..1995)
                     /gene="grxC"
                     /locus_tag="VGM46_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008598069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEY4193463.1"
                     /translation="MADVTIYTRAMCGYCAAAKRLLDRKGVTYTEHDASFSPELRQEM
                     ISRAKGGSTFPQIFVGDVHVGGSDDLHALEAAGRLDALLAGPQA"
     gene            complement(2021..2794)
                     /locus_tag="VGM46_12530"
     CDS             complement(2021..2794)
                     /locus_tag="VGM46_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEY4193464.1"
                     /translation="MADIKGVSRVVLKWPARLLFPAVCAGCRRQVSQPGVLCAQCWPK
                     LRLLERPWCPVMGTPFIHDMGDGFLSAEAIADPPPFGRVRAAAAYSGVARQMVQGLKY
                     QDRTDLAPWMARWMMRAGAELVEEADIVVPVPLHWRRFFKRQFNQSAELGRAVSKLGG
                     LPFSPSAIRRVKMTSQQVGLERREREENVRAAFRVPVEAEIQIAGRRVLLIDDVYTTG
                     ATVRAVAKALVKGGAGAVDVLTFARVLPGDFQPDESATI"
     gene            2856..3737
                     /locus_tag="VGM46_12535"
     CDS             2856..3737
                     /locus_tag="VGM46_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY4193465.1"
                     /translation="MQPIFDTPLFLARKRRALAGPVAGADFLMRRVAEDFTDRLDAVE
                     RRFEKAAALFCQTPDAADAMMASGKVGEIIRIEADATFLGRHAGTIAAPETVPLEPQS
                     IDLAVSLLSLQTMNDIPGMLAQIRRSLRPDGLFLAAFAGSGTLTELRDSLLAAEAELY
                     GGVSPRVIPFPDVRDAGALLQRAGFALPVSDVEDVVVRYDSLFSLMGDLRAMGETNAL
                     IDRPRRPGTRALFARAAEIYAQRFSDADGRIRASFSLVWMSGWAPDPSQRKPLQPGSA
                     KVSLASILEASARREAD"
     gene            complement(3765..3956)
                     /locus_tag="VGM46_12540"
     CDS             complement(3765..3956)
                     /locus_tag="VGM46_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEY4193466.1"
                     /translation="MKILIRRFCQARFFQDRRGATAIEYGLIAALLSLAIFAGFGNVA
                     DRLEWLWSDNSSRLVQGMN"
BASE COUNT          766 a         1354 c         1220 g          715 t
ORIGIN      
        1 cagcgtcacg atcggcgaca gcgtgaacat gatgccggta tagccggcgc cgagatgcga
       61 aatcgctgaa aatattaaaa ggttcggaat tgcgtaggag acggcggcga ccacgacgaa
      121 ataccgcagc ttgtgcggcg tcagccgaat gcgattgccg cgcagcaggt ggacgcaaag
      181 caggatgccg ccggcgccga gcgaaatgac gaacgcccac acgatgccgg caacgccggc
      241 gttggttgcc atccggccca agggcagaga gacgccaagc aacgtgcctg tgatgatcag
      301 cagggcgaac gcggaatccc aggtgaactt catttcaaac ccgtcatggg cgcaggccgg
      361 tcaagggcgc gagccggtcg aacgcggatg gggaaaatgc acggaccata gagccgggag
      421 gctcagttgc ggtcgtcggg aaacaccggg acgggcatga aagccacgcc gtcctcggca
      481 aggctcttgg cctcgtcgaa ggtcgcttcg ccataaatgc cccgcgcatc ggcttcgccg
      541 aaatggattt ttcgggcttc ctcggcgaat ttgtcgccca cataatccgc attctcacgt
      601 attttttcgg ccatcgcctt catctgcgcc atcgcctgct tctgggcttc acccatggcg
      661 agcgcgattt tctcctgctt gcggcccgtc gacaccgccg gcgccatcag cgccttttcg
      721 accttgtgcg aaccgcatga cgggcagtcg acaaggccgc gcaaggcctg cttgtcgaaa
      781 tcttcattgc tgcgaaacca gccttcgaac tcatgggcat tttcgcaagt aagggaaaac
      841 cggatcaaga cgccgctccc ctgagcgtac ccacgcccgc gccgctttcg gtagccaacg
      901 caaactcccg cgcattcttc aggttgggaa tcttccggcg cgcggcctgc gattgcgccg
      961 gatcgatttc ggcgacgatg acgcccggct cgtcatgatc cacttcggca agcaccctgc
     1021 cccatgggtc cacgatcagc gaatggccat aggtttcgcg gccgtcctca tgcagcccgc
     1081 cttgcgcggc ggcgaccacg aacgccccgt tttcaatggc gcgggcgcgc agcaagacat
     1141 gccaatgcgc ctcgcccgtc tggcgggtga aggcagccgg aaccgtcagg atttcagcgc
     1201 ccgccaaggc ctcggcccgg aacagttgcg gaaagcgcag gtcgtagcag acggaaaagc
     1261 cgagcttcgt tccgttcagg tcggccacga ccgcctgtgc gcccggctcg taaaccgccg
     1321 attcgcgcca gctctcgccg ttgtcgagat cgacgtcgaa catgtggatt ttgtcatagc
     1381 cggcaacaag cccgccttgc ggcgaaaaaa gataagcgcg gttcgccacc ttgccgtcgg
     1441 cgcgcaaaat ggcggtcgag cctatatgca ggaagatgcc cagttcgccg gccagacggc
     1501 gcgcggccgc caccaccttg tcattggcct cgacggcgaa tatcgctttg cgcgtattcg
     1561 cggcgcggac catcgcaccg gtcatttcgg gggtctggac gtaggtcgct ccctggcccg
     1621 ccgcctcgcg caccagccgc tccatttcca ccacgttgcg ctgcggatcg gtgccggacc
     1681 gcatctggac cgcggcaacc ttgaaaacgc ccatcgtcac ggtccttgtt atgcctgcgg
     1741 gccggcaagc agcgcatcga ggcgtcccgc cgcctccagc gcgtgcagat cgtcgctgcc
     1801 gcccacatgc acatcgccga cgaatatctg cggaaacgtc gagccgcctt ttgcccgcga
     1861 gatcatctcc tggcgcagct cgggcgaaaa gctggcatcg tgctcggtat aggtcacgcc
     1921 cttgcggtcg agcaggcgct tggccgccgc gcaatagccg cacatcgcgc gggtataaat
     1981 ggttacatca gccatgaggt cattccagtt ttgctgccct ctatatagtt gcgctttcgt
     2041 cgggctgaaa gtccccaggc aaaacccgcg cgaaggtcag cacgtcgacc gcgccggccc
     2101 cgcccttgac cagcgccttg gcaaccgccc gcacggtggc cccggtcgta tagacatcgt
     2161 cgatcagcag aacccggcgg ccggcaatct ggatttccgc ctccaccggc acgcgaaacg
     2221 cagcgcggac gttttcctcg cgctcgcgcc gttccagtcc gacctgctgg cttgtcattt
     2281 tcacgcgccg gatcgccgat ggcgaaaacg gcagcccgcc gagttttgaa acggcgcgcc
     2341 caagctcggc cgattggttg aactgccgct tgaagaagcg ccgccaatgc agcggcaccg
     2401 gcaccactat gtcggcctcc tcgaccagtt cggcgccggc ccgcatcatc cagcgcgcca
     2461 tccatggcgc caagtcggtc cggtcctggt atttcaggcc ttgcaccatc tggcgcgcga
     2521 cgccggaata agccgccgcc gcccgcacac gtccgaacgg aggcgggtcg gcgatcgctt
     2581 cggctgacag aaacccatcg cccatgtcgt gaatgaaagg cgtgcccatc accgggcacc
     2641 acggccgctc caaaaggcgc agctttggcc agcattgcgc gcacagcacg cccggctgcg
     2701 agacttgccg gcggcaaccc gcgcaaaccg ccggaaacag cagacgcgcg ggccatttga
     2761 gaaccactct ggaaacgccc ttgatatccg ccattggatc ggccacctga ggaacgatgc
     2821 atcctagcag atgccggaaa tacaggaacc tgtccgtgca gccgattttc gatacgccgc
     2881 tgttcctcgc acgcaaacgc cgcgcgcttg ccggccccgt cgccggagcg gatttcctga
     2941 tgcgccgcgt cgctgaagac ttcaccgacc ggctggatgc cgtggagcgc aggttcgaga
     3001 aggcggcggc gctgttttgc cagacgccgg atgccgccga tgcaatgatg gccagcggca
     3061 aggtggggga gattatccgt atcgaggccg acgcaacttt tctcggccgc catgccggta
     3121 ccatcgcggc gccggaaacc gtgccgcttg aaccgcaaag catcgatctt gccgtctccc
     3181 tgctgtcgtt gcagaccatg aacgacattc cgggcatgct ggcgcagata aggcgctcgt
     3241 tgaggccgga cggcctgttt ctggctgctt ttgccggctc aggcaccctg accgagttgc
     3301 gtgacagcct tctggccgcc gaggccgaac tatatggcgg cgtcagcccg cgcgtcattc
     3361 cattccccga cgtgcgcgac gccggtgcgc tgctgcaacg ggccggcttt gccttgcccg
     3421 tctccgatgt cgaggacgtg gtggtgcgtt acgattcgct gttttcgctc atgggcgatc
     3481 tgcgggcgat gggtgaaacc aacgcgctga ttgaccggcc gcgccgcccg ggcacgcgcg
     3541 cgcttttcgc ccgcgccgcc gaaatctacg cgcaacgctt ttcagacgcc gacgggcgca
     3601 tcagggcaag cttttctcta gtctggatgt ccggctgggc gcccgaccca tctcagcgca
     3661 agccgctcca gccgggttcg gcaaaagttt ccctggcttc gatattggaa gcgtccgcac
     3721 gccgcgaagc ggactaagtc gccgcaatca gaggcaagcc aggcctaatt catgccctgc
     3781 accaggcggc tactattgtc ggaccacagc cattcgagcc tgtctgccac attgccgaag
     3841 ccagcaaaga tggccagcga cagcaacgcc gcgatcaagc catattcgat ggccgtcgcc
     3901 ccgcggcgat cctgaaaaaa ccgggcctgg caaaaccggc gaatcaaaat tttcatggcc
     3961 gtcgatcccc tcgccggatc attggtatgc aagacgtgtt aaggaaagtt aacggcgcaa
     4021 gcttaacagg ccataaaccg gcaagctgcg cggta
//
LOCUS       DASYCS010000403         2039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2579475,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000403 DASYCS010000000
VERSION     DASYCS010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2579475"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="VGM46_12545"
     CDS             complement(<1..741)
                     /locus_tag="VGM46_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEY4193467.1"
                     /translation="MPKKPAAIPVAAQSAAAKTAPATGPAANVAAGTSMKGQHVFLVD
                     GSGYIFRAYHALPPLTRKSDGLPVGAVAGFCNMLWKLLRDMPKDGKPTHLAIIFDKSE
                     VTFRNKLYPAYKAHRPPAPEDLIPQFALIREAVKAFDLPCIEQSGFEADDLIATYVRE
                     ACERGATATIVSSDKDLMQLVTDCVTMYDTMKDRRIGIPEVIEKFGVPPNKVVEVQAL
                     AGDSVDNVPGVPGIGIKTAAQLITEYGDL"
     gene            complement(1013..2035)
                     /locus_tag="VGM46_12550"
     CDS             complement(1013..2035)
                     /locus_tag="VGM46_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEY4193468.1"
                     /translation="MKNERVDWIDYAKGICIIMVVMMHSVQGVEKAAGQDGFMHLVVA
                     FAKPFRMPDFFLISGLFLSLVINRDWRTYLDRKVVHFAYFYLLWTAIQFGFKAPHFAA
                     EVGWPGVARLYLMSFIDPFGTLWFIYLLPVFFVVIKATRNVSPWAIWGAAAALEMAHI
                     STGWTLIDEFAARFVYIYSGYLFASYVFALSNRARAYPALALAALALWAVTDAGLVYT
                     GASEWPLISLALGLAGACAIITIGTLLAKTGWLGFVRYCGEHSIVIYLAFFLPMAASR
                     TILLKAGVIHDIGLVSLIVTIVGVIGAVLIWWTCRNTRLNFLFERPDAFWIAPKKRTP
                     ALQAAE"
BASE COUNT          416 a          602 c          657 g          364 t
ORIGIN      
        1 gaggtcgcca tattcggtga tgagctgcgc cgcggtcttg atgccgatgc ccggtacgcc
       61 cggcacgtta tcgacgctgt cgcccgccag cgcctgcacc tcgaccacct tattgggcgg
      121 tacgccgaac ttctcgatca cttcgggaat gccgatgcgg cggtccttca tggtgtcgta
      181 catcgtgacg caatcggtga cgagctgcat caaatccttg tccgaggaga cgatggtcgc
      241 ggttgcgccg cgctcgcagg cttcgcgcac gtaggtcgcg atcaggtcgt cggcttcaaa
      301 tccgctctgc tcgatgcagg gtagatcgaa ggctttcaca gcctcgcgga tcagcgcgaa
      361 ctgcggaatc agatcctcgg gagcgggcgg ccggtgcgcc ttgtacgcgg gatagagctt
      421 gttgcggaac gtcacctcgg acttgtcgaa gatgatcgcg aggtgggtcg gcttgccgtc
      481 cttcggcatg tcgcgcagca acttccacag catgttgcag aagcccgcga cggcgccgac
      541 cggaaggccg tcggatttgc gcgtgagcgg cggcagcgca tggtaggcgc ggaaaatgta
      601 gccggaaccg tcgaccagaa aaacgtgctg gcctttcatg ctggtgcctg cggcgacgtt
      661 cgcggcagga ccggttgcgg gggcggtttt ggctgcggca gattgcgcgg cgacgggtat
      721 ggcggctggc tttttcggca tggccgcaac ttaaggattt ttgccgcgaa tagcaccccc
      781 ctagtacccg ctttccgacg ttcgtgagac gtcgcaacac ccgccttaac gaacttcgga
      841 aagcaaaggg tactagcaac tttatgattc tggtgccgct ttcgatttga aattcccgaa
      901 gacgaatccg ctgcagctgg tgcaggaatt tcaaatcggc ggtaccagaa gcgtcatcgc
      961 cagcaaaatc gcagcattgc ggaccttggc ctggtgcgcc ggcggagctg ccttactccg
     1021 ccgcctgaag cgcgggtgta cgcttcttcg gcgcgatcca gaacgcatcg gggcgctcga
     1081 acaggaagtt cagccgcgtg ttgcggcagg tccaccagat cagcaccgcg ccgatcacgc
     1141 cgacgatggt gacgatcaac gataccagac cgatgtcgtg gatgacgcct gctttcagca
     1201 ggatcgtgcg ggaggcggcc atcggcagga agaaggcgag atagatgacg atggaatgct
     1261 cgccgcaata gcgcacgaat ccaagccaac ccgtcttggc cagcagcgtg cctatcgtga
     1321 tgatggcgca ggcgccggcg agccccagcg cgagcgaaat cagcggccat tcgctggcgc
     1381 cggtatagac gagaccggca tccgtgaccg cccacagcgc cagcgccgcc agcgccaatg
     1441 ccgggtaggc gcgggcgcgg ttcgacagtg cgaacacgta gcttgcgaac agatagccgg
     1501 aatagatgta gacgaagcgg gcggcgaatt cgtcgatcag cgtccagccg gtggagatat
     1561 gcgccatctc gagtgccgcg gcggcgcccc agatcgccca tggcgaaacg ttgcgcgtgg
     1621 ccttgatgac cacgaagaac accggcagca gatagatgaa ccacagcgtg ccgaacggat
     1681 cgatgaacga catcaggtaa aggcgcgcta ctcccggcca accgacctcg gcggcaaagt
     1741 ggggcgcctt gaagccgaac tggatcgcgg tccacagcag gtagaaatag gcgaagtgca
     1801 ccaccttgcg gtcgagatag gtccgccagt cgcggttgat gacgagcgac aggaacagtc
     1861 cggaaatcag gaagaaatcc ggcatccgga acggttttgc gaaggcgacc acgagatgca
     1921 tgaagccgtc ctgcccggcg gccttttcga ccccctgcac cgaatgcatc atgacaacca
     1981 tgatgatgca gatgcccttg gcatagtcga tccagtcgac ccgttcgttt ttcagcgtg
//
LOCUS       DASYCS010000404         5858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_5040486,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000404 DASYCS010000000
VERSION     DASYCS010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 5858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5858
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5040486"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="VGM46_12555"
     CDS             complement(<1..645)
                     /locus_tag="VGM46_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY4193469.1"
                     /translation="MRIFLAGASGVVGRRLVPLLLARGHLVWGTTRSEAKAAQLGDLG
                     AIPVMVDVFDRQKLIEAVVTAAPQVVVHQLTDLPENLDPSKMQAAIAGNARIRDEGTR
                     NLVEAARQAGVSRVVAQSIAWAYAAGPLPHGEDDRLDAKAEGARGITMGGVIALERQV
                     LGAAGIEGVVLRYGQFYGPGTGVDTPAGTSPVHVDAAAHAALLAVDRGRAGIYNI"
     gene            complement(771..1220)
                     /locus_tag="VGM46_12560"
     CDS             complement(771..1220)
                     /locus_tag="VGM46_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEY4193470.1"
                     /translation="MRERIAEEVKTAMKARDADRLPTLRLMQSAIKDRDIANRGLGKE
                     AASDDEILQILAKMVKQREESAKAFEDGGRPELAAKERAEIAILRDFQPVQMDDAGVQ
                     AVAREAIAATGAATGKDMGKVMAVLKEKYAGQMDFSKASGVVKGLLG"
     gene            1440..2642
                     /gene="carA"
                     /locus_tag="VGM46_12565"
     CDS             1440..2642
                     /gene="carA"
                     /locus_tag="VGM46_12565"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006338169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEY4193471.1"
                     /translation="MATTAPWSTELVTALLVLADGTVIEGRGLGAVGSAVAEVCFNTA
                     LTGYEEILTDPSYAGQIVTFTFPHIGNIGANNEDIEDLNPVARAGAVGAVFKADVTDP
                     SSYRAKEHLDEWLKKRGIVALSGIDTRALTALIREKGMPNAVIAHAPDGKFDVADLKR
                     RAAAWSGLVGLDLAKEVTSGQSSVWRETPWEWNEGFGEQDAPTMHVVAIDYGIKRNIL
                     RLLAGLGAKVTIVPAKTGADEILAMKPDGIFLSNGPGDPAATGEYAVPVIQDLLKTEI
                     PVFGICLGHQMLAIAVGGKTEKMHQGHHGANHPVKDHTTGKVEIVSMNHGFAVDGKSL
                     PQGVEETHVSLFDGSNCGIALTGRPVFSVQHHPEASPGPQDSHYLFRRFVNLIRARRN
                     EPALAERA"
     gene            complement(2651..3919)
                     /locus_tag="VGM46_12570"
     CDS             complement(2651..3919)
                     /locus_tag="VGM46_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006338170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HEY4193472.1"
                     /translation="MIEPKLARRGLVLVFTTLLLDIVGFGIIMPVLPAYLRELTGVGI
                     SEAAIKGGWLFFVYAVMQFFFAPVMGGLSDRFGRRPILLASVLTFAIDNLICAVAWSY
                     SMLFIGRVLAGISGASYSTTSAFIADISNDDNRAKNFGLLGIAFGVGFIIGPVLGGLL
                     GTFGPRVPFYFAAGLAFVNFLIALFLLPETLEQKHRRRFEWKRANPVGTLLQMRQYEG
                     IGWIGLAFFLMTLGHMMYPAVWSFVSNYRYGWSETQIGLSLGAFGLCGALVMGLVLPR
                     VIPRLGEWKTAAIGLAFTALGALGYALAWQGWMVYAVIVVGCLEALADPPLRSLAAAK
                     VPPSAQGELQGAMTSIFSITSIVTPLLYTAVFSWFTGPNAPVIFGGAPYVLGAFFLVL
                     ALVVFVTRVKRPTPREVVQMHQENAAAAEG"
     gene            complement(4155..5081)
                     /locus_tag="VGM46_12575"
     CDS             complement(4155..5081)
                     /locus_tag="VGM46_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008877560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEY4193473.1"
                     /translation="MLWKGRRQSDNIEDQRGQSGGGLPGGFGGGPGQFRIPIGGRAGG
                     GGLSTIVILVVLFFALKACGIDPMQILAGGDAGGNGGQVTRQTSNNPGAPASDDEMKQ
                     FVGTVLAETEDVWGGIFKANGLTYEDPKLVMFSGQIRSACGFATSASGPFYCPGDHKV
                     YLDTAFFQQLSRQFGASGDFAQAYVVAHEVGHHVQNLTGILPKFNQMRQRMSEADANH
                     MSMQVELQADCFAGVWGHFTAQKGILEQGDLEEALNAAQQIGDDTLQKKTQGYVVPES
                     FNHGTSAQRQKWFAAGFKSGKLSDCDTFNNPI"
     gene            complement(5313..5501)
                     /locus_tag="VGM46_12580"
     CDS             complement(5313..5501)
                     /locus_tag="VGM46_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193474.1"
                     /translation="MGVEQAPTAKGKASAKGLRQAAAKDERKTEAETGKPLKKGEKRF
                     EERSRSSDGKSAGAKQKD"
     gene            complement(5609..>5858)
                     /locus_tag="VGM46_12585"
     CDS             complement(5609..>5858)
                     /locus_tag="VGM46_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron dependent repressor, metal binding and
                     dimerization domain protein"
                     /protein_id="HEY4193475.1"
                     /translation="AKMLTRLCADGLVSRKPYRGVFLTEAGRKMAEESRIRHQTVEAF
                     LRSLGVSAETARIDAEGIEHHVSSETLEAFRCAMTKAH"
BASE COUNT         1105 a         2002 c         1714 g         1037 t
ORIGIN      
        1 gatattatag atgcccgccc ggcctcgatc gaccgcgagc agggccgcat gggccgccgc
       61 atcgacatgc accggcgatg tgcctgcggg cgtgtccact cccgtgccgg gaccgtaaaa
      121 ctggccgtaa cgcagcacca ccccttcaat gccggcggcg cccagaacct ggcgctccaa
      181 cgcgatcaca ccacccatgg tgatgccccg cgcgccttcc gccttggcgt cgagcctatc
      241 gtcctcgcca tgcggaagcg gacccgccgc ataagcccag gcgatgcttt gcgccaccac
      301 gcgggaaacc cctgcctgac gggcggcctc gaccagattg cgggtgccct cgtcgcggat
      361 gcgggcattg cccgcgatgg cggcttgcat tttcgacgga tcgagatttt cgggcaagtc
      421 cgtaagctgg tggacgacga cctgcggcgc agctgtgacc accgcttcga tcagtttctg
      481 cctgtcgaac acatccacca tcaccggtat cgccccaaga tctccaagct gtgcggcctt
      541 ggcttcggac cgggtggttc cccagacgag atggccgcgc gcaagcaaga gcggcaccag
      601 ccgccggccg acgacaccgg atgcgccggc aagaaaaata cgcatatgcg ttcattccag
      661 gctacgaaat ttcggtccga ttaatatccg ggcaccgaga tttttcaaga caacgctctg
      721 ccattgctgc gactcgaata atatagttcg accttgaacg tcgatcggcc ctagccgagc
      781 aagcccttga cgacgccgct tgccttggag aaatccatct ggccggcata tttttccttg
      841 agcacggcca tcaccttgcc catgtccttt cccgtcgcgg cgcccgttgc ggcaatcgcc
      901 tcgcgcgcca ccgcctgaac gccggcatcg tccatctgca ccggctggaa atcgcgcagg
      961 atcgcaatct cggcgcgctc cttggcggcc agttccggcc gcccgccatc ttcgaacgcc
     1021 ttggccgatt cctcgcgctg cttcaccatc ttggccagga tttgcaggat ctcgtcgtcg
     1081 ctcgccgcct ccttgccaag gccgcgattg gcgatgtcgc gatccttgat cgccgactgc
     1141 atcagccgaa gcgtcggcag ccggtcggcg tccctcgcct tcatcgcggt tttgacttcc
     1201 tcggcgattc gctcacgcat tttctgctcc tttgaatggc gcgcacccta tcgccgcgaa
     1261 aaacccagcg caatcgccgc cccgctcatt gaccgctttc ggcccttccc ctatgtgaag
     1321 gccttgcaac cagaatggtt acgcgtggag gctgcgacca tcgccgctcc gcgctttttg
     1381 ctgcgccaat cgcgccgtcg gcgaacgaaa ccggccgcag cctgttgatg gagagccgca
     1441 tggccacgac cgccccctgg agcaccgaac tcgtcaccgc cctgctcgtg ctggcggacg
     1501 gcacggtgat cgaaggccgc ggcctcggcg cggtcggctc ggcggtggcg gaagtctgct
     1561 tcaacacggc gctgaccggc tatgaggaaa tcctcaccga cccatcctat gccggccaga
     1621 tcgtcacctt caccttcccg catatcggca atatcggcgc caataacgag gatatcgaag
     1681 atttgaaccc cgtcgcccgc gccggcgcgg tcggcgccgt gttcaaggcc gatgtcaccg
     1741 acccgtccag ctaccgcgcc aaggaacatc tcgacgagtg gttgaagaag cgtggcatcg
     1801 ttgccctttc cggcatcgat acccgcgcac tgaccgcgct gatccgcgaa aaaggcatgc
     1861 ccaacgccgt catcgcccac gcgcccgatg gcaagttcga cgtggccgac ctgaagcggc
     1921 gcgccgccgc ctggtccggc cttgtcgggc tcgacctcgc caaggaggtc acctccggcc
     1981 agtcgtcggt ctggcgcgag acgccatggg aatggaacga aggctttggc gagcaggacg
     2041 cccctaccat gcatgtcgtc gccatcgact acggcatcaa gcgcaacatc ctgaggctgc
     2101 ttgccgggct gggcgccaag gtcaccatcg tgccggccaa gaccggcgcc gacgaaatcc
     2161 tcgccatgaa gccggacggc atcttcctgt ccaacggccc cggcgaccct gccgccaccg
     2221 gcgaatatgc ggttccggtc atccaggatt tgttgaagac cgaaatcccc gtcttcggca
     2281 tctgccttgg ccaccagatg ctggccatcg ctgtcggcgg caagacggaa aagatgcatc
     2341 agggccacca cggcgccaat cacccggtca aggaccacac caccggcaag gtcgagatcg
     2401 tctcgatgaa ccacggcttc gccgttgacg gcaagtcttt gccgcaaggc gttgaggaga
     2461 ctcacgtttc gctattcgat ggctcaaact gcggcatcgc gctgaccgga cgcccggtgt
     2521 tctcggtcca gcaccatccc gaagcctcgc ccggcccgca ggattcgcac tatctgttcc
     2581 gccgcttcgt caacctgatc cgcgcgcgcc ggaacgaacc ggcgctggcc gagcgggcct
     2641 gagcgaccgc tcagccttcg gcggccgccg cattttcctg gtgcatctgc acgacctccc
     2701 tcggcgtcgg ccgcttcacc ctggtgacaa agaccacgag cgccagcacc aggaagaaag
     2761 cgcccagcac ataaggcgcg ccgccgaaga ttaccggcgc gttcggcccg gtgaaccatg
     2821 agaacacagc cgtgtagagc aacggcgtga cgatcgaggt gatcgaaaat atcgaggtca
     2881 tcgcgccctg caattcgccc tgcgccgatg gcggcacctt ggcggcggca aggctgcgca
     2941 gcggcggatc ggcaagcgct tcaaggcagc cgaccacgat gaccgcatag accatccagc
     3001 cctgccaggc caacgcatag cccagcgcgc caagtgctgt gaaggcaagg ccgatggccg
     3061 ccgtcttcca ttcgccgagc ctcgggatca cccgcggcag caccaacccc atgaccagcg
     3121 cgccgcacag gccgaacgcc cctagcgaaa gcccgatctg cgtttcgctc cagccataac
     3181 ggtagttgct gacgaacgac cacaccgccg gatacatcat gtggccaagc gtcatcagga
     3241 agaaggcaag gccgatccag ccgatgcctt catattggcg catctgaagc agggtgccga
     3301 ccgggttcgc ccgcttccat tcgaagcggc ggcgatgttt ttgctccagt gtttccggca
     3361 gcagaaacag cgcaatgagg aagttgacga acgccaggcc ggcggcgaaa taaaacggca
     3421 cgcgcggccc gaacgtgccg agcagcccgc ccagcaccgg accgatgatg aagccgacgc
     3481 cgaaggcgat gccaagcagg ccgaaattct tcgcccggtt gtcgtcgttc gagatatccg
     3541 cgatgaaggc cgaggtcgtc gaatagctgg cgccggaaat gccggccagc acgcggccga
     3601 taaacagcat cgaatatgac caggcaaccg cgcagatcag gttgtcgatg gcgaaggtca
     3661 gcaccgaggc cagaaggatg ggccgccggc cgaaccggtc gctcaagccg cccatcaccg
     3721 gcgcgaagaa aaactgcatc accgcataga cgaagaacag ccagccgccc ttgatcgcag
     3781 cctcgctgat gccgacgccc gtcaactcgc gcagataggc cggcagcacc ggcatgatga
     3841 tgccgaagcc gacaatatcg agcaaaagcg tggtgaagac gagaacgagg ccgcgccggg
     3901 ccagttttgg ttcgatcatg atcgtttgtc cgctgaaact ccccgcaacg atggcgatca
     3961 accctatatg gacgcgctca taagggaaca ggccggaatg tcttttgccg gcgcatgccg
     4021 ccagcagtct tcgagaacgc ctcccggtta ggggaaacgc tccagccacg cagtacccgg
     4081 cattcacgca ggccggcaaa tgccggcctt ggacgatgca gccgatcagc cttcaggccg
     4141 atatcaaagc cgcatcagat cggattgttg aacgtatcgc agtccgagag cttgccgctt
     4201 ttgaaaccgg ccgcgaacca tttctggcgc tgcgccgaag tgccgtggtt gaagctttcc
     4261 ggcacgacat agccttgcgt cttcttttgc agcgtgtcgt cgccgatctg ctgggcggcg
     4321 ttcagcgcct cttcgaggtc gccctgctcc aatatgcctt tctgcgcggt aaaatggccc
     4381 cagacgccgg caaagcagtc ggcctgcaat tccacctgca tcgacatgtg gttggcgtcg
     4441 gcctcgctca tccgctgccg catctggttg aacttgggca ggatgcccgt caggttctgc
     4501 acatggtggc cgacttcgtg cgccaccaca taggcctgcg caaaatcgcc cgaagcgccg
     4561 aactgccgcg acaattgctg gaagaaagcc gtgtccagat agaccttgtg gtcgccgggg
     4621 caatagaacg gtccggaagc cgaggtggcg aaaccgcagg cagaccggat ctggccggaa
     4681 aacatcacca gcttggggtc ctcataggtc aggccgttgg ccttgaagat gccgccccac
     4741 acatcctcgg tttccgccag cacggtgccg acgaactgct tcatctcgtc atcactggcg
     4801 ggcgcgcctg gattgttgct ggtctgccgg gttacctggc cgccattgcc gcccgcatcg
     4861 ccgccggcaa ggatttgcat cgggtcgatg ccgcaggcct tcagtgcgaa gaacaagacg
     4921 acaagaatga cgatggtcga caacccgcca ccgccggcgc ggccgccgat cggaatgcgg
     4981 aactgccccg gaccgccgcc gaaaccgccc ggcaatccgc cgccgctttg cccgcgctgg
     5041 tcttcgatat tgtcactctg acgacggccc ttccagagca tggctacccc tcatacatgc
     5101 gggccgactg tggcggccgg ttgcagcagt ccagttttca acgattatcc gaatgactgc
     5161 ggcaagtcac agcatttttc gcaaacgccg ccgatcttgc ccgttcaagg caaggcaaac
     5221 ctcgtcgccg caagcctgcg cggcaatcgg catatatctg gccagcggcc tttagcgcca
     5281 gcccatgacc tcgcctcgat gcaagaaagc gttcagtctt tctgcttggc gccggcactc
     5341 ttgccgtcgg agcttctgga acgctcttcg aaacgcttct cgcccttctt caaaggcttg
     5401 ccggtttcag cctcggtctt gcgctcatcc ttggcggcgg cctgccttag tcccttggcc
     5461 gaagccttgc ccttcgcggt cggtgcctgt tcgacgccca tttcatttcc tccgctggcc
     5521 cgcgccaacc gcgggcatca cagtcaacgc atggtcgata cggcggttcc ggcgccgcct
     5581 ggcaggcctg gtgcggccat gcggacagtc aatgtgcctt ggtcatggcg cagcggaacg
     5641 cctccagcgt ctcgctgctg acatggtgct cgatgccctc ggcatcgatg cgcgcggttt
     5701 cggcgctgac gccgagcgaa cgcaggaacg cctcgaccgt ctgatggcgg atgcggcttt
     5761 cctcggccat cttgcgccca gcctcggtca ggaaaacgcc gcgatagggc tttctggaaa
     5821 ccagtccatc tgcgcaaagc cgcgtcagca tcttggcg
//
LOCUS       DASYCS010000405         3375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_697200,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000405 DASYCS010000000
VERSION     DASYCS010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697200"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VGM46_12590"
     CDS             <1..496
                     /locus_tag="VGM46_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY4193476.1"
                     /translation="DITSLGGTVVLCMVTLAAVVYLFVTRQPRTALFVLVSVVGGQAL
                     SSALKLAVDRPRPELVSHLVHVSTLSFPSGHAMMSAVTYLTLGALAARFVHGHAARIY
                     LLTLAVLMTLMVGVSRVYLGVHWPSDVFAGWCAGFAWALLCWLAARLWQRRRGSAGDP
                     PPET"
     gene            complement(499..972)
                     /locus_tag="VGM46_12595"
     CDS             complement(499..972)
                     /locus_tag="VGM46_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEY4193477.1"
                     /translation="MAKEIERKFLVANGSWLAAAEKKIAIVQFYLAISAERSIRLRIS
                     DGRKARLTLKFGSDLTERDEFEYPVPLDEATEMMGFAIGTTIRKTRHHVRHMNYLYEV
                     DVFNGDLDGLVVAELETPDLVADAMLPGWLGREITGEIRYSNASLALNGFPEPVR"
     gene            complement(1113..2339)
                     /locus_tag="VGM46_12600"
     CDS             complement(1113..2339)
                     /locus_tag="VGM46_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014329672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEY4193478.1"
                     /translation="MVEANPANGLIADRTRAGGPASIAAVGMALTAYPVAVTRGFISR
                     QEARLRTLTTLRFFWNSPQGPEKDATGHKGFYYHFLDMQSGRRVWKSELSTVDTALLM
                     AGILVAAAFFEEDTEDEHEIRTLADALYRRVDWAWAQNGGAAITHGWRPERGFLPYRW
                     IGYDEALILYVLALGSPTHPPSAECYVEWLTGYRWKKIYGQEYLYAGPLFIHQMSHLW
                     IDFRGIQDAFMRDHDSDYFENSRRATYVQRQYAIDNPRGFKGYHANCWGITASDGPGK
                     QKRVIDGIERRFHGYRARGVPFGPDDGTFSPWATVASLPFAPEIVLPALRYFHEIDLK
                     DDHPYGFTASFNPTLGAPGGPAFGWMSPEHVGINQGPIALMIENYRSGLIWKLMRECP
                     YIIAGLSAAGFRNGWL"
     gene            complement(2571..3311)
                     /gene="msrA"
                     /locus_tag="VGM46_12605"
     CDS             complement(2571..3311)
                     /gene="msrA"
                     /locus_tag="VGM46_12605"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEY4193479.1"
                     /translation="MTERRNSIGKRLPGSSGTAFAALALATAGFAWWQMPAKAAEDAV
                     VIPPPSMDEKAAGGTETAVFAGGCFWGVQGVFQHVKGVDSAVSGYTGGSADTAVYETV
                     GSGSTGHAESVKITYEPSKVTYGQLLQVYFSVAHNPTQLNYQGPDQGTQYRSTIFAQN
                     DSQKKIAQSYIAQLDKAKTFDKPIVTTLETGKKFYPAEGYHQDFLTLNPTYPYIVYND
                     LPKVENLKRLFPAKYSEKPVLVSAQAKS"
BASE COUNT          590 a         1045 c         1043 g          697 t
ORIGIN      
        1 cgacataacc agccttggcg gcacggtcgt cctgtgcatg gtgacgctgg cggcggtggt
       61 ctatctgttc gtgacccgcc agccgcgcac ggcgctgttc gtgctggttt ccgtggttgg
      121 cgggcaggcc ctgtccagcg cactgaagct cgcggtagac aggccgcgcc ccgagcttgt
      181 ctcgcatctc gtgcatgttt ccacgctgtc ttttccgagc ggccacgcca tgatgtcggc
      241 ggtgacctat ctgacgctgg gcgcgctggc ggcacgcttc gtgcacggtc acgccgcccg
      301 catctatctg ctgacgctcg ccgtgctgat gacgctgatg gtcggcgtca gccgtgtcta
      361 tctcggcgtg cactggccct cggacgtgtt tgcgggctgg tgcgccggct ttgcctgggc
      421 gctgctgtgc tggctggccg cgaggctctg gcagcgtcgg cgcggatcgg cgggcgaccc
      481 gccgccggag acctgatttc agcggacagg ctccggaaag ccgttcagcg ccagcgacgc
      541 gttggaatag cggatttcgc cggtgatttc gcgcccgagc cagccgggca gcatcgcatc
      601 ggccaccaga tcgggtgttt ccagctccgc caccaccaac ccgtcgagat cgccattgaa
      661 cacatctact tcgtagaggt agttcatgtg gcgcacatgg tggcgggtct tgcggatggt
      721 ggtgccgatg gcaaaaccca tcatctccgt cgcctcgtcg agcggcaccg gatattcgaa
      781 ctcgtcgcgc tcggtgagat cgctgccgaa cttcagagtc agcctggcct tccggccgtc
      841 gctgatgcgc agccggatcg agcgttcggc cgagatcgcc aggtagaact ggacgatggc
      901 gattttcttc tcggccgcgg caagccacga tccattcgcc accagaaact tgcgctcgat
      961 ttccttagcc atcgccggcg actaaagccc gcccggccgc ccaaatcaat cgcggcaatt
     1021 gccaaaccgg atcattgttg cgtgagtttc aaaagtcagc gatgacttaa atgcgcctgg
     1081 aacgggcaat cgacccgatt tcagcttcgc gctcacagcc agccgttcct gaatcccgcc
     1141 gcgctcaggc cggcgatgat ataggggcat tctcgcatca gcttccagat gaggccgctg
     1201 cggtaatttt cgatcatcag cgcgatcggc ccctgattga tgccgacatg ctccggcgac
     1261 atccagccga aagccgggcc gccgggtgcg cccagtgtcg gattgaagct ggccgtgaag
     1321 ccatagggat ggtcgtcctt gaggtcgatc tcgtgaaaat agcgcagcgc cggcaatacg
     1381 atctccggcg cgaagggcag cgacgccacc gttgcccacg gtgaaaaggt gccgtcatcc
     1441 ggcccgaacg gcacgccgcg cgcgcgatag ccgtggaagc gccgctcgat gccgtcgatc
     1501 acgcgtttct gcttgcccgg cccgtcgctg gcggtaatgc cccagcaatt ggcgtgatag
     1561 cctttgaagc cgcgcggatt gtcgatcgca tattgccttt gaacataggt cgcacggcgg
     1621 ctgttttcga aataatcgct gtcatgatcg cgcatgaaag cgtcctggat gccgcgaaaa
     1681 tcgatccaca gatgcgacat ctgatggatg aacagcggcc cggcatagag atattcctgg
     1741 ccgtaaatct tcttccagcg atagccggtc agccattcga catagcattc ggccgatggc
     1801 gggtgcgtcg gcgagccaag cgcgagcacg tagagaatca gcgcctcgtc atagccgatc
     1861 cagcgatagg gcaggaagcc gcgttccggc cgccagccat gggtgatggc cgcgccgcca
     1921 ttttgcgccc atgcccaatc gacgcggcgg taaagcgcat cggcgagcgt tcgtatctcg
     1981 tgctcatcct ctgtgtcttc ctcgaaaaag gccgcggcaa ccaatatgcc ggccatcagt
     2041 agcgcggtat ccacggtcga aagctcggat ttccacaccc tgcggccgct ctgcatatcg
     2101 aggaagtggt agtagaaacc cttgtgcccc gttgcatcct tttcaggccc ttgcgggctg
     2161 ttccagaaaa agcgcagcgt cgtcagcgtc ctcagacgcg cctcttgtcg cgagatgaag
     2221 ccgcgcgtca ccgcgacggg ataggcggtc agcgccatgc cgaccgccgc gatgcttgcc
     2281 gggccgccgg cgcgcgtgcg gtctgcgatc aatccgttcg ccgggttggc ctcgaccatg
     2341 aagtaccgga aggaaatttc ctggagcgat gccagttggg cgtcgtccgc tgcaagcggg
     2401 ccggtggcgc tatccaagtt caactgcccc tttgacgaac cgataaagga aaagccggct
     2461 ggccgggtca ggttccgcat gcgggtttga aacgaggctc aaggctgcat ccagccgggc
     2521 aattcccggc cggatggtcg tcatcatcgg cttgttccgg cctgcgccaa tcagcttttg
     2581 gcctgcgccg aaaccagcac cggcttttca ctgtatttcg ccgggaacaa ccgcttgagg
     2641 ttttccacct tgggcaggtc gttgtagacg atatagggat aggtcggatt gagcgtcagg
     2701 aaatcctgat gatagccttc ggccggatag aatttcttgc cggtttcgag cgtggtgacg
     2761 atcggcttgt cgaacgtctt ggccttgtcg agctgggcga tatagctctg cgcgatcttc
     2821 ttctggctgt cgttttgcgc gaagatggtc gagcgatatt gcgtgccttg gtccggtccc
     2881 tgatagttca actgggtcgg attgtgcgcg accgagaaat acacctgcaa taactggcca
     2941 taggtgacct tcgacggctc gtaggtgatc ttcacggatt ccgcatgccc ggtgctgccc
     3001 gagcctacgg tctcgtaaac cgccgtgtcg gcgctgccgc cggtatagcc ggaaaccgcg
     3061 ctgtcgacac ccttcacatg ctggaacacg ccctgcacgc cccagaagca gccgccggcg
     3121 aataccgccg tttcggtgcc gccggcggct ttttcatcca tgctcggcgg cggaatgacc
     3181 accgcgtcct cggcggcctt ggccggcatc tgccaccacg cgaagcctgc ggtagcgagc
     3241 gccagcgcgg cgaacgcggt tccagatgaa ccgggcaggc gttttcctat cgaattcctt
     3301 cgttcagtca tggttggggg ctccatagca tccttagagt ttcgatctta cgcgatagat
     3361 agggtattat cgtct
//
LOCUS       DASYCS010000406         5081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_698699,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000406 DASYCS010000000
VERSION     DASYCS010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 5081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5081
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698699"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VGM46_12610"
     CDS             complement(<1..192)
                     /locus_tag="VGM46_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193480.1"
                     /translation="MAVDDPDIKFDIFRLWVIGRQFPNSDDYWDGNWLNVRAEVRANG
                     AIIEVIGAILHLSELASFAS"
     gene            complement(212..1270)
                     /locus_tag="VGM46_12615"
     CDS             complement(212..1270)
                     /locus_tag="VGM46_12615"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY4193481.1"
                     /translation="MQPLSGLLVLDFTTLLPGPLASLMLAEAGAEVIKIERPGGEDMR
                     HFEPHIDGESVPFAMLNRGKQVLTIDLKNEADRDKLMPLIKRADILIEQFRPGVMARF
                     SLGYDDVAKINPKLIYCSISGCGQSGPRADEAGHDINYIGNTGVLDLQPGPIDRPVVP
                     PMLVADIAGGSFPAVINILLALRSRDQSGQGSHLDIAMTDAMFTFAWYALALGAAAKF
                     PKSGEMWLVGSSPRYQLYPTKDGRIVACGAIEQKFWDSFVTAIGLAAAFADDKRNPTE
                     TRDEVAKLIAAKTSGEWRPILAAADCCATIVAPLEEAMRDPHFVGRGLFAHQAEMASG
                     KSVLALPLPIAPAFRKLS"
     gene            complement(1289..2641)
                     /locus_tag="VGM46_12620"
     CDS             complement(1289..2641)
                     /locus_tag="VGM46_12620"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY4193482.1"
                     /translation="MEWGDVIKIVAPALAVLGAVVALTRYVTQLQDRLDTNNQIQEFK
                     IKVAALEQAAELRAAQHESELKVLRDDMLRLSDQNRTIEERYRLAIEASSAVAALKTS
                     VDAEMQRLADRLGASAHSILVPNPSVVPNDPVDHLVFLCLVPENQELRHERISLSSVA
                     GQVLNVPKPVITHDPATAGAFSKQTDLVSGLKTANMLVVPLLHKGRCIGVAEFLNKRS
                     GLSFDPNDIDVAEETAQALAPKVGDFIADPINMKALGFTPRQRPTDATILVSDISNSS
                     KLAKTLDVSVVIDLLNQYMEALCDVGMRFGGTVEQLQGDGFIMTFNVRRPLDQHHSAA
                     LGAALEMQKRFRELKAKWINLQYPGMQNVFNRIGLTSGPVNKAELGPPQFRQITVIGD
                     AVNAAAHLCQAGSRDRDVIVAGNAFYQRLDVKPEAMEVALPVTRDNALENAFDLTIPA
                     "
     gene            complement(2713..3675)
                     /locus_tag="VGM46_12625"
     CDS             complement(2713..3675)
                     /locus_tag="VGM46_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEY4193483.1"
                     /translation="MIDVINLALPFFGLIFIGFACGKLKQIPDTALAWMNFFIVYVSL
                     PALFYRILAQTPLEQLARADFVLATTLSTFCAFALAFIVGMAIRHRHIGESTITGLAG
                     GYGNIGYMGPGLALATLGPEAAVPVALIFCFDTLLLFTLVPVLMALATPGQRDAGAVV
                     KEAVTRIVTNPLVIATALGILSAALHFQPPVALERLMAFLQNAAAPCALFTLGVTVAL
                     RPLKKIPWEVPLTTAIKLAAHPVLMFLLLSLLGPFDQLWVYAAVLMAALPPALNVFVF
                     ARQYDTWVEEASSAVLVGTLVSVVTLTSVMWMVKTHTLPPLLFR"
     gene            3809..4849
                     /locus_tag="VGM46_12630"
     CDS             3809..4849
                     /locus_tag="VGM46_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013652982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEY4193484.1"
                     /translation="MKLPRQFFQPLAIGAPAPMRDLPVRLERMIHFVPPHMEKMRAKV
                     PEIARQVDVVLGNLEDAIPVEAKEAARAGFIEMGKSTDLGQTGLWTRINALNSPWALD
                     DITEIVGAIGDKIDVIMLPKVDGPWDIHYLDQLLAQLEARHKVTKPILIHAILETAQG
                     VNNVEAIATASPRMHGMSLGPADLAASRAMKTTRVGGGHPEYKVLEDAKGDAPRAAYQ
                     QDLWHYTTAKMVDACAAAGIKPFYGPFGDFSDAAACEVQFRNSFLLGCVGAWTLHPSQ
                     IAIAKKVYAPDPAEVAFAKKIVDSMPDGAGAVMIDGKMQDDATWKQAKVLLDLAKLVA
                     AKDPEMGASYGL"
     gene            4905..>5081
                     /gene="hspQ"
                     /locus_tag="VGM46_12635"
     CDS             4905..>5081
                     /gene="hspQ"
                     /locus_tag="VGM46_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019858091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HEY4193485.1"
                     /translation="MRTAKFTIGQVVKHRLFSFRGVIFDIDPVFDNTDEWYEAIPAEV
                     RPRKDQPFYHLFAEN"
BASE COUNT         1131 a         1541 c         1434 g          975 t
ORIGIN      
        1 gctggcgaac gacgctaact ccgagagatg tagaattgcg ccaattacct cgatgatagc
       61 cccgtttgct ctaacttcag ctcgaacgtt gagccagttt ccatcccaat aatcatcaga
      121 gtttgggaat tgacgaccaa ttacccaaag gcgaaaaata tcaaacttaa tatctggatc
      181 atctacagcc attgtcgagg cagcaccttc tctaagacaa tttacgaaac gccggagcga
      241 ttggcagagg caaggccagc accgattttc ctgacgccat ttccgcctga tgcgcgaaga
      301 ggccacgtcc gacaaagtga ggatcgcgca ttgcctcttc cagaggtgca acaatcgtcg
      361 cgcagcaatc ggcggcggca agaatgggcc gccattcgcc cgacgttttt gcagcgatta
      421 gctttgcgac ctcatcgcgg gtctctgttg gattgcgttt gtcatcggca aatgcagccg
      481 ccaatccaat tgccgttacg aaactatccc agaatttctg ctcgatggcg ccgcaggcaa
      541 cgatacggcc gtctttggtc ggataaagct gatagcgtgg ggatgagcca accagccaca
      601 tctcgccaga cttcgggaat ttcgctgctg cgccaagcgc gagcgcgtac caggcgaagg
      661 tgaacatcgc gtcggtcatg gcaatatcga gatgcgagcc ctgcccgctt tgatcgcgcg
      721 agcgcaacgc cagcagaata ttgatcaccg ccggaaagct gccgccggca atgtcagcca
      781 cgagcatcgg cggcacgacg ggccgatcga taggcccggg ctgcaaatcg agcacgccgg
      841 tgttgccgat gtaattgatg tcgtgcccgg cctcatcggc tcgcgggccg ctctggccgc
      901 aacccgagat cgagcaataa atcagcttcg gattgatctt agcgacgtcg tcatagccaa
      961 ggctgaagcg cgccatgacg ccgggacgga actgctcgat cagaatgtcg gcgcgcttga
     1021 tcagcggcat cagtttgtcg cgatccgctt cgttcttgag gtcgatcgtc agcacctgct
     1081 tgccgcgatt gagcatcgcg aacggcacgc tctcgccgtc aatgtgcggc tcaaaatgcc
     1141 gcatgtcctc gccgcccggc cgctcgatct tgatcacttc ggcgcccgct tccgccagca
     1201 tcagcgacgc cagcggtccc ggcagcagcg tggtgaaatc cagaaccaga aggccggaca
     1261 acggttgcat aaatgatcta acgtcgccct aggcagggat tgtcaaatcg aaggcattct
     1321 caagcgcgtt gtcgcgcgtg actggcagcg cgacctccat cgcctccggc ttcacatcca
     1381 gccgctgata aaaagcattg cccgcgacaa tgacatcccg gtcacggctg ccggcttggc
     1441 aaaggtgcgc tgccgcgttt acggcatcgc caatgacagt gatttgcctg aactgaggcg
     1501 gcccaagctc cgccttgttg accggcccgc tggtaagtcc aattcgattg aaaacattct
     1561 gcatgcccgg atattgcaga ttgatccatt tggccttcag ttcccggaac ctcttctgca
     1621 tttcaagtgc ggcacccagc gccgccgagt gatgttggtc aagcgggcgc cgaacattga
     1681 aggtcataat gaacccgtct ccctgcaatt gctcaacggt accgccgaaa cgcattccaa
     1741 catcacaaag cgcttccatg tattgattga gaagatcaat aacgacggaa acatcgagcg
     1801 tcttggcaag cttggatgag tttgaaatgt cactcaccag aatcgttgcg tccgtcggac
     1861 gctgacgcgg agtaaatcca agtgccttca tatttatcgg gtcggcgatg aagtcaccca
     1921 ctttcggcgc caacgcctga gctgtctctt cagcgacatc gatatcgttt gggtcaaatg
     1981 agagtccgct acgcttgttt aagaattcgg caactccgat gcatcgccct ttatgcaaca
     2041 atggaacgac caacatattt gcagtcttaa gacctgatac caaatcggtc tgcttggaga
     2101 atgcgccggc cgttgctgga tcgtgcgtaa taacaggttt cggcacattc agcacctgac
     2161 ccgccacgct cgatagcgat atccgctcat ggcgcagttc ctgattctcc ggcaccaaac
     2221 agaggaatac gagatgatcg accggatcat ttggcaccac cgaagggttt ggcaccaaga
     2281 ttgaatgcgc gctcgcacca agccggtcgg caagccgctg catttcggca tcgacagaag
     2341 tcttgagcgc ggcaacggcg ctacttgcct cgattgctaa gcggtaacgc tcctcaatcg
     2401 tacgattttg atccgacagg cggagcatat catctcgcaa cactttcaat tcgctctcgt
     2461 gctgtgcggc tcgcaattcc gcggcctgtt cgagcgccgc gaccttgatt ttgaattcct
     2521 gaatttgatt gtttgtatcg agacgatctt ggagctgggt cacgtaacgt gtaagcgcta
     2581 cgactgcgcc aagcacggca agcgccggcg caacgatctt aatcacatcc ccccattcca
     2641 tcgacaaact cgctcttgga taaaggcgat actatcgcag gccgcccgtc ccgaatagag
     2701 gaggaaaacg aattacctga acagtagtgg aggcagtgtg tgcgttttca ccatccacat
     2761 cacgctggtc agcgtcacca ccgataccag ggtgccgaca agtacggcgc tcgatgcctc
     2821 ctcgacccat gtatcgtatt gccgcgcgaa cacgaacaca ttgagcgccg gcggcagcgc
     2881 ggccatcaac acagcggcgt agacccacaa ttgatcgaat gggcccaaca acgacagcag
     2941 cagaaacatc agcaccggat gcgcagcgag cttgatcgcg gtcgtgagcg gcacttccca
     3001 tggaatcttc ttcaaaggcc gtagtgctac cgtaacgccg agcgtgaaca gcgcgcacgg
     3061 cgcggcggca ttttgcagga atgccatcag ccgttcgagc gcgaccggcg gctggaaatg
     3121 cagcgccgct gacaggatgc caagcgccgt tgcgatcacc agcgggttgg tgacgatgcg
     3181 cgtgacggct tccttcacca cagcaccggc gtcgcgttgt cctggtgtag cgagagccat
     3241 cagcaccggg accagagtga acagcagcag cgtgtcgaaa cagaagatca gcgcaaccgg
     3301 tacggcagct tccgggccga gcgttgccag tgccaggccc ggccccatgt agccaatatt
     3361 gccgtatccg ccggcgagac cggtgatcgt tgactctccg atgtggcgat gccggatcgc
     3421 catgccgacg atgaatgcca gcgcgaaagc gcagaaggtc gacaacgtcg tcgccagcac
     3481 gaagtcggca cgcgccaatt gctccagcgg cgtctgcgcc aggatgcggt agaacaaggc
     3541 gggcagcgac acatagacga tgaagaagtt catccaggct agcgccgtat cgggaatctg
     3601 cttgagcttg ccgcaggcga aaccgatgaa aatcagcccg aaaaacggca gcgccaggtt
     3661 gatgacgtcg atcatttcag gctccggcgg ccgtcaaacg cgcccgctct acgcgaaact
     3721 tggcgcggcc tttgctttcc tctaaacggc agaaagcccc tttggaaggc caatcccttt
     3781 agaaggcaaa tccttcagga agactgtcat gaagcttccg cgtcagtttt ttcagccgct
     3841 cgccatcggc gcgccggccc ccatgcgcga cctgccggtg cggctggagc gcatgatcca
     3901 tttcgtgccg ccgcacatgg aaaagatgcg ggccaaggtg ccggagatcg ccaggcaggt
     3961 cgacgttgtg ctcggcaatc tggaagacgc cattccggtc gaggccaagg aggcggcccg
     4021 cgccggcttc atcgaaatgg gcaagtcgac cgacctcggc cagaccggac tctggacccg
     4081 catcaatgcg ctgaactcgc cctgggcgct cgacgatatt accgagatcg tcggcgccat
     4141 cggcgacaaa atcgacgtca tcatgctgcc gaaggtcgac ggtccgtggg acatccacta
     4201 cctcgatcag ttgctggcgc agctcgaagc gcgccacaag gtcacaaagc ccatcctgat
     4261 ccacgccatt ctcgagacgg cgcaaggcgt gaacaacgtc gaagccatcg cgaccgcctc
     4321 gccgcgcatg cacggcatgt cgcttggacc tgccgatctc gcggcctcgc gtgcgatgaa
     4381 gaccacgcgc gtcggcggcg gccatccgga atacaaggtc ctggaagacg ccaaaggaga
     4441 cgcgccgcgt gcggcctatc aacaggacct ttggcattac acgacagcca agatggtcga
     4501 cgcctgcgcc gcggccggca tcaagccgtt ctatgggccg ttcggcgact tctccgacgc
     4561 ggcggcctgc gaagtgcagt tccgcaattc gttcctgctc ggctgcgtcg gcgcctggac
     4621 gctgcacccc tcgcagatcg ccattgccaa gaaggtctat gcgccggacc cggcagaggt
     4681 ggccttcgcc aaaaagatcg tcgattccat gccggacggc gccggcgcgg tcatgatcga
     4741 cggcaagatg caggacgacg cgacctggaa gcaggccaag gtgctgctcg acctcgccaa
     4801 attggttgcc gccaaggacc cggaaatggg tgccagctat ggcctataag gcgcgcggcc
     4861 tagccaaaag cagtcagatc aactatttgg cgggcatgga aaagatgcgt accgccaaat
     4921 tcacgatagg acaggtcgtc aaacaccgcc tgttttcgtt ccgtggcgtg attttcgata
     4981 tcgacccggt cttcgacaac actgacgagt ggtacgaggc gattcccgcc gaagtacgcc
     5041 cgcgtaagga tcagccgttc taccacctgt tcgccgaaaa c
//
LOCUS       DASYCS010000407         1679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4898478,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000407 DASYCS010000000
VERSION     DASYCS010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4898478"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1149)
                     /locus_tag="VGM46_12640"
     CDS             complement(<1..1149)
                     /locus_tag="VGM46_12640"
                     /inference="COORDINATES: protein motif:HMM:NF019935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEY4193486.1"
                     /translation="MAELENQRAVLDRRRLRGELDALAERSGGNAAGATEDLQAILRG
                     HLAEGRAEIRRRFDANPRGGGSGLAAAHETSFMIDQIIRCLFDYAVEHAYPLPNPTSA
                     ERLGLAAVGGYGRGELAPASDIDLLFLLPYKLTPHGEQVVEFILYRLWDLGLKVGHAS
                     RSVDECIRQAQGDLTIRTNLLEARYLWGDQKIYEQFHNRFDREIVKGKGDAFLKDKLA
                     ERHSRHARTGNSRYTLEPNIKEGKGGLRDLQTLLWAGRFMFGVTGFEQLIDKELLTPQ
                     EVATYEKAMNFLWTLRCHLHYLTGRAEDRLTFDLQPEMARRLGYTDHAGTRGVERLMK
                     HYFLVVKSVGGLTRYFLAAVESKRLHRPLFRMPMIGWLKSEVEGFRNDG"
     gene            complement(1194..>1679)
                     /locus_tag="VGM46_12645"
     CDS             complement(1194..>1679)
                     /locus_tag="VGM46_12645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193487.1"
                     /translation="TKVDCSQIDMKFSAPGYEAKCTDFSDATIDVNGEATGARKTEQI
                     LAVSSDTYLVAVDDRPLGSRIFIKRSGLEENVERFFPNTRVTDWSPANTVAGFEIGEF
                     TGGPESINQMNCIGFRRVVSYHLQGLGRVVVGVSCSDQSVKHAVKHAYDALGKLQAPG
                     G"
BASE COUNT          316 a          519 c          526 g          318 t
ORIGIN      
        1 gccatcattg cggaagcctt cgacctccga tttcagccag ccgatcatcg gcatgcggaa
       61 cagcggccga tgcaggcgct tcgattcgac ggcggccagg aaatagcggg tcaggccgcc
      121 caccgatttc accaccagga aataatgctt catcaaccgc tcgacgcccc gcgtgccggc
      181 gtggtcggta tagccgaggc gccgcgccat ttccggctgc aggtcgaagg tgaggcggtc
      241 ttcggcgcgg ccggtgagat agtgaagatg gcagcgcagg gtccagagaa agttcatcgc
      301 cttttcatag gtggcgactt cctgcggggt caggagctcc ttgtcgatga gctgctcgaa
      361 tccagtcacg ccgaacatga agcggccggc ccagagcagg gtctgcaggt cgcgcagccc
      421 gcctttgccc tccttgatat tgggctccag cgtgtagcgc gaattgccgg tgcgggcatg
      481 gcgcgaatgc cgctcggcca gcttgtcctt caggaacgca tcgcccttgc ccttgacgat
      541 ctcgcggtcg aagcggttgt ggaactgctc gtagattttc tggtcgcccc agagatatcg
      601 cgcctccagg agattggtgc ggatggtgag gtcgccttgg gcctggcgta tgcattcatc
      661 caccgaacgc gaggcatggc ctaccttcaa accgagatcc cagagccggt agagaatgaa
      721 ctccaccacc tgctcgccat ggggcgtcag cttatagggc agcaggaaca ggagatcgat
      781 atcggaagcc ggagccaact cgccgcgccc gtagccgccg accgcggcca ggccaagccg
      841 ttcggcgcta gtcgggttgg gaagtggata ggcgtgttcg acggcatagt cgaagaggca
      901 gcggatgatc tggtcgatca tgaagctggt ttcgtgggcc gccgccaggc cggacccgcc
      961 gccgcgggga ttggcgtcga accggcggcg gatttccgcg cgcccctccg ccagatgacc
     1021 gcgcaggatc gcctgcagat cctcggtcgc gccggcggcg ttaccgccgc tccgctcggc
     1081 aagggcgtca agctcgccgc gcaggcgtcg gcggtctaga acagcgcgct ggttttcaag
     1141 ctcggccatg ccgccatgct acgcgccgcg cgcaacatgg taaagcgagg gcgttatccg
     1201 ccgggcgcct gaagtttccc caaagcgtca taggcatgtt tgacggcatg tttgacgctt
     1261 tgatcggaac aggaaacacc cacgacaacc cgccccagcc cctgcagatg gtatgagacc
     1321 acgcgccgga acccgataca gttcatctga ttgatactct cgggaccgcc ggtgaactcc
     1381 ccgatttcaa aacctgcaac ggtattggcc ggcgaccaat cggtgacccg ggtgttcggg
     1441 aaaaaccgct ccacgttttc ctcgaggccg ctgcgcttta taaaaatccg gctgccgagc
     1501 gggcgatcgt caacggcgac cagataagta tcgctggaaa ccgcgagaat ctgttccgtt
     1561 ttgcgcgcgc cggtggcctc tccattgaca tcgattgtcg catcgctgaa gtccgtacat
     1621 ttggcttcgt agcctggcgc cgaaaatttc atgtcgatct ggctgcaatc gaccttagt
//
LOCUS       DASYCS010000408         4093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3454308,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000408 DASYCS010000000
VERSION     DASYCS010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3454308"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="VGM46_12650"
     CDS             complement(<1..211)
                     /locus_tag="VGM46_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006327477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4345 domain-containing protein"
                     /protein_id="HEY4193488.1"
                     /translation="MEYSFPWPMSQGEWLAWSSAVVTVLLGLLLFLAPGISFRVLRLQ
                     AKPEKPAAIAEARGRMSGFYLGVGLC"
     gene            complement(328..2511)
                     /locus_tag="VGM46_12655"
     CDS             complement(328..2511)
                     /locus_tag="VGM46_12655"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEY4193489.1"
                     /translation="MSNDSSFHAAVPVLVPMPAERPYTYAVPAGMRVEPGSIVRVPLG
                     PRQVAGIVWDGTVENIDARKLRAIEQVFDCPPIPEPMRRFVDWVAQYTLSPPGMVARM
                     LLRAPEAFAPEPWTEGLQRTQAEPDRMTTARARVLDTAEGGLAWTRTGLAHAAGVSST
                     VVDGLKAQGVFETMMIPPRPVVAAPDPTYAVPELMPDQQGAAYMLRAGVEAAAFNVTL
                     LDGVTGSGKTEVYFEAVAAALDQGKQVLILLPEIALTHAFLERFQDRFGAKPGEWHSD
                     LPPRMREKVWRQTAEGTLKVVAGARSALFLPFKDLGLIVVDEEHDPAYKQEDRVFYNA
                     RDMAVVRAHIGGFPVVLASATPSVESRVNAGQGRYARVVLSGRFAEAAMPQLKSIDMR
                     RSPPARGGFLSPLLLGAMRQTLERQEQSLLFLNRRGYAPLTLCRVCGHRFGCPVCSAW
                     LVEHRFRGQLVCHHCGHNEKRPEACPECGTFDHLVACGPGVERIAEEVVQHFPDARTI
                     VLSSDMMGGVRRLRLELEAIAKGEADIVIGTQLVAKGHNFPNMTLVGVVDADLGLANG
                     DPRAAERTFQLLSQVTGRAGRTGKKSLGLLQTYQPEHPVMRAIVSGDSDTFYEREIAE
                     RERAILPPFGRLAGIIVSAASRAEAEGHARGLRRAAPEATDIFVLGPAEAPLALLGGR
                     HRFRLLIQGEKRADMQGFVRTLLTAGPKVRGSVRVQVDIDPQSFL"
     gene            2602..3255
                     /gene="fsa"
                     /locus_tag="VGM46_12660"
     CDS             2602..3255
                     /gene="fsa"
                     /locus_tag="VGM46_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEY4193490.1"
                     /translation="MKFFVDTADIKDIRELNDLGLVDGVTTNPSLILKSGGKIDEVTR
                     QICEIVDGPVSAEVTATEYSEMMKEAKVLAKIADNIAIKVPLTFDGLKACKAIRSEGR
                     MVNVTLCFSATQALLAAKAGATFISPFVGRLDDTGIDGMELIAEIRQIYDNYDFDTEI
                     LVASVRTTNHVKQAALIGADVATVPPATLKALVKHPLTDKGLEQFLADWAKTGQKIG"
     gene            complement(3367..3936)
                     /gene="lptE"
                     /locus_tag="VGM46_12665"
     CDS             complement(3367..3936)
                     /gene="lptE"
                     /locus_tag="VGM46_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018430160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HEY4193491.1"
                     /translation="MSLPERRKMNVTLAVRALALGGLIAAGGLVSACTVRPLYSNAPL
                     SAGSQVGAAAELASIAVKPVTTRYAQQVRNNLIFAFTGGAGEPASPAYSLNLGVTELV
                     QSAALIQVTTNEDTPTAGTVTLKAAYTLTDAKTGKTVSSGRRSITSSFDRPLQEFASY
                     RAQRDAEDRAARELSDLLHIAVAQDLAKR"
BASE COUNT          731 a         1323 c         1361 g          678 t
ORIGIN      
        1 aacagaggcc aacaccgaga taaaatcccg acatgcggcc ccttgcctcg gcgatcgccg
       61 ccggcttttc cggtttggct tgcagccgca gaacccgaaa cgagatgccg ggcgccagaa
      121 acagcaacag gccaagcagc accgtcacca ccgcgctcga ccaagccagc cactcgccct
      181 ggctcatcgg ccacggaaaa gaatattcca tcagctgccc ctctcctcaa tccgcaattt
      241 ttgcatcttt acccatcccg ttttgtgacg ccagcgcggg cggacggaat ttgcgcacag
      301 gcacggctgt agtcacaaaa gcccccctca caagaagctt tgcgggtcga tatcgacctg
      361 cacgcgcacc gagccgcgta ctttcgggcc ggccgtcagc agggtgcgta cgaagccttg
      421 catgtcggcg cgtttttcgc cctggatcaa caggcggaag cggtggcgtc cgcccagcag
      481 cgccagcggc gcttcggcgg ggcctagcac gaatatgtcg gtggcttcgg gcgcggcgcg
      541 acgcaggccg cgcgcgtggc cttctgcttc ggcgcgcgac gcggcgctga cgataattcc
      601 ggccagacgg ccgaagggcg gcaggatggc gcgttcgcgc tcggcgatct cacgctcgta
      661 gaacgtgtca gaatcgccgg aaacgatggc ccgcatcacc ggatgttccg gctgataggt
      721 ctgcaacagg ccaaggcttt tcttgccggt gcggccggcg cggccggtga cctgcgacag
      781 caactggaag gtgcgctcgg cggcgcgcgg atcgccattg gcaaggccga ggtcggcatc
      841 gacgacgccg accagcgtca tgttcgggaa attatgcccc ttggcgacaa gctgggtgcc
      901 gatgacgatg tcggcctcgc ccttggcgat ggcttcgagt tccagccgca gccggcgcac
      961 gccgcccatc atgtcggagg aaagcacgat ggtgcgcgcg tcgggaaaat gctggacgac
     1021 ttcctcggcg atgcgctcga cgccgggacc gcaggcgacg agatgatcga acgtgccgca
     1081 ttcggggcag gcttcgggcc gcttttcatt atggccgcaa tggtggcaga cgagctggcc
     1141 gcgaaagcgg tgctcgacca gccatgcgga gcagaccggg cagccgaagc ggtggccgca
     1201 gacccggcac agcgtcagcg gcgcatagcc gcgccggttg aggaacagca gcgattgctc
     1261 ctgccgctcc agcgtctgcc gcatcgcgcc aagcagcagc ggcgaaagaa aaccgccgcg
     1321 cgccgggggc gagcggcgca tgtcaatgga tttcagctgc ggcatcgcgg cttcggcgaa
     1381 gcgacctgaa agcacgacgc gcgcgtagcg gccctgtccg gcgttgacgc ggctttcgac
     1441 cgagggggtg gcagaggcga gaacgacggg aaagccgcca atatgggcgc gcaccaccgc
     1501 catgtcgcgc gcattgtaga acacgcggtc ttcctgcttg taggcggggt cgtgctcctc
     1561 gtcgacgacg atgaggccga gatccttgaa gggcagaaac agcgccgagc gcgcgccggc
     1621 gacgaccttc agcgtgcctt ccgccgtctg ccgccagacc ttttcgcgca tgcggggcgg
     1681 caggtcggaa tgccattcgc cgggcttggc cccgaagcga tcctggaagc gttcgaggaa
     1741 cgcgtgggtc agcgcgattt ccggcagcag gatcagcacc tgcttgccct gatcgagggc
     1801 cgcggccacc gcctcgaaat agacctccgt cttgccggag ccggtgacgc cgtcgagcag
     1861 ggtgacgttg aacgcagccg cttccacgcc ggcgcgcagc atgtaggcgg caccctgctg
     1921 gtcgggcatc agctcgggca cggcataggt cgggtcgggc gcggccacga cggggcgagg
     1981 cgggatcatc atcgtttcga acacgccctg cgccttcagc ccgtcgacca ccgtggatga
     2041 gacgccggcg gcgtgcgcca ggccggtgcg cgtccaggca aggccgccct cggcggtatc
     2101 cagcacgcgc gcccgcgccg tggtcatccg gtcgggttcg gcctgcgtgc gttgcaagcc
     2161 ctcggtccat ggctcagggg cgaaggcctc cggcgcgcgc agcagcatgc gcgccaccat
     2221 gcccggcggc gacagcgtgt actgcgccac ccagtcgacg aagcggcgca ttggttccgg
     2281 gatcggcggg cagtcgaaca cctgctcgat ggcgcgcagt ttccttgcgt cgatgttttc
     2341 caccgtgccg tcccagacga tgccggcaac ctggcgcggc ccgagcggca cgcgcacgat
     2401 cgaacccggc tcgacgcgca tgcctgcggg aaccgcatag gtgtagggcc gttcggctgg
     2461 catgggaacc aacaccggca ctgctgcatg aaaggacgaa tcgttgctca tcgcgcgtga
     2521 ccttgcccgt agtttggttt agagcaaagg ccaacgccga gcgtgccctc ccgacacccg
     2581 gaaaacacca aggagatcgc catgaaattt tttgtcgaca ccgccgatat caaggatatc
     2641 cgcgaactga acgatctggg tctggtcgat ggggttacca ccaacccgtc gctgatcctg
     2701 aaatcgggcg gcaagatcga tgaggttacc cggcagattt gcgagatcgt cgacggtccg
     2761 gtttcggcgg aagtgaccgc caccgaatat tcggaaatga tgaaggaagc caaggtcctg
     2821 gccaagatcg ccgacaatat cgcgatcaag gtgccgctga ccttcgacgg gctgaaggcc
     2881 tgcaaggcga tccgttcgga aggccggatg gtcaatgtca cgctgtgctt ctccgccacc
     2941 caggcgctgc tggccgccaa ggcgggcgcc accttcatct cgcccttcgt cggccgtctc
     3001 gacgataccg gcatcgacgg catggaactg atcgccgaga tccgccagat ctacgacaat
     3061 tacgatttcg acaccgaaat cctggttgct tccgtgcgca ccaccaacca cgtcaagcag
     3121 gcggcgctga tcggcgcgga cgtggcgacg gtgccgccgg cgacgctgaa ggcgctggtc
     3181 aagcacccgc tgaccgacaa ggggctggag cagttcctgg ccgattgggc caagaccggc
     3241 cagaagatcg gctgagcggc tcccggagac tgtcgataac gttgcccgcc cgcgccggat
     3301 gaccggccgg gcggcgtttg cgaagaggtg ctcggcgctt ggccggtcgt atcgcctggc
     3361 ccggacctag cgtttggcca ggtcctgggc gacggctatg tgcaggaggt cggataattc
     3421 gcgcgcggcg cggtcttcgg catcgcgctg cgcccggtag gaggcgaatt cctgtagtgg
     3481 cctgtcgaag gacgacgtga tcgagcggcg gcctgaactt accgtcttgc ccgtcttggc
     3541 atcggtcagc gtataggcgg ctttaagggt aaccgtaccg gctgtcggcg tgtcctcgtt
     3601 ggtcgtcacc tggataaggg cggccgactg gacaagctcg gtgacgccga gatttagcga
     3661 ataggcgggc gatgccggtt cgccggcgcc gccggtgaag gcgaatatca gattgttgcg
     3721 cacctgctgg gcgtaacggg tcgtcaccgg cttgacggca atcgaagcca gttcggcagc
     3781 cgcgccgacc tgcgaacccg ccgacagcgg cgcgttcgaa tagagcggcc gcaccgtgca
     3841 ggccgacacc aggccgcccg ccgcgatcag gccgccaagc gccaaggcgc gcacagccag
     3901 cgtcacgttc atcttcctgc gttcaggcaa cgacattgac gatcctctgg gggacgatga
     3961 taaccttgcg cggcgctttt ccttcgagcg ccttctgcac gaagtccaaa gccaggacgg
     4021 ttttttcgat ttcaccttgg tctgcgtcgc gggcaattgt caaatctccg cgcttcttgc
     4081 cgttcacctg cac
//
LOCUS       DASYCS010000409         3685 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4470312,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000409 DASYCS010000000
VERSION     DASYCS010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4470312"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(224..1435)
                     /locus_tag="VGM46_12670"
     CDS             complement(224..1435)
                     /locus_tag="VGM46_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019858578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HEY4193492.1"
                     /translation="MGNSRVKALLGTAFLSLAMGGAAYAGGFSRGSADTDLIYEDGNF
                     NMRSSVTYVSPHREFSKNPNPALVGTSYTEDYMVPSAAAKFNLTDNLRCVGTYVQNNG
                     GSARYDFPTYSGKTMEEFTTYEAAGTCGVSFDAGQGKFWLLGGGYIETLDYYRENSYG
                     PFGNAQLSLDGRKFGYRIGAAYEIPELALRGQVMYRSGTDYGAQGTATAPAGVLAGAL
                     ARQGVPNGANPFLALPATAAVPVAATGVGNLPQSVDIKFQTGIAPGWLAFGAVKWTDW
                     STLSTLDVRSVKGGFLLSSDQYHWKDGWTVTGGVGHKFNDAVSGLVSLTWDQGVATGW
                     DLQSDSYTLASGVALKDKLGGELRGGVGFTYIDSATETKNFDPITGADQNQAVKAGYA
                     VALNLGYNIKW"
     gene            complement(1814..>3685)
                     /locus_tag="VGM46_12675"
     CDS             complement(1814..>3685)
                     /locus_tag="VGM46_12675"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019170484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEY4193493.1"
                     /translation="TVEAAININDNEDFLAGLDSTPERQAVWNELHGLDRFAARKRIV
                     ELMEEGGFLDKIEPHRHQVPHGDRGGVPIEPFLTDQWYVNAAELAKPAIASVREGRTS
                     FVPKNWEKTYFDWMENIQPWCISRQLWWGHQIPAWYGPDGAVFVEKTEEAALDAAIQH
                     YLAHEGPWKAWVEERLENFQPGEILTRDEDVLDTWFSSALWPFSTLGWPDKTPELKTY
                     YQTDVLVTGFDIIFFWVARMMMMGLHFMDEEPFHTVYVHALVRDKNGAKMSKSKGNVI
                     DPLELIDEYGADALRFTLTSMAAQGRDVKLDPARIAGYRNFGTKLWNATRFAEMNGVA
                     RNDDLWLGDAKLAVNRWILTELTRATRAITEGITSYRFNEAAGAAYRFVWNMVCDWYL
                     ELLKPVFMGDDEEAKVESRACVAFVLDEIYKLLHPMMPFMTEELWAQTAGEGQQRQSL
                     LAHAAWPAPDFEDEEAASDINWLIDLVTGIRSVRSEMNVPPAAIAPLVVVGANDTTRE
                     RLARHDPAIKRLARIGEISLADAAPKGSAQIVLNEATVSLPLGSLIDLAAEAARLQKE
                     LAKATDEIARLHKKLSNDKFVANAPDDVVAAEREKLAEYRETQEKLSVALGKVRDAG"
BASE COUNT          719 a         1135 c         1118 g          713 t
ORIGIN      
        1 gagacctgat tgaggtgctc cgggtttatg gcgggaaatg ggcaaattcg ggcaggccag
       61 ggttcgcgca gcgggatgac acgcgtgtgt tgccaaaacg acacagccga ttgaagcgag
      121 attggcagag caggcctttt ccgacattca aatgaccgga aaaaaggccg agagacccgg
      181 cctttttctc agcgaccagc cttttttcag cgatttgaat cgcttaccac ttgatgttgt
      241 agcccagatt caaggccacc gcataaccgg ccttcaccgc ctggttctgg tcggcgccgg
      301 tgatcgggtc gaagttcttc gtttccgtcg cggagtcgat gtaggtgaag ccgacgccgc
      361 cgcgcaattc cccgcccagc ttgtccttga gcgcaacgcc gctcgccagc gtataggaat
      421 cggactggag atcccagccg gtggcgacgc cctggtccca ggtcaggctg acaaggccgg
      481 aaaccgcgtc gttgaacttg tggccgacac cgccggtaac cgtccagccg tctttccagt
      541 gatattgatc ggaggaaagc aggaacccgc ctttgaccga gcgaacgtca agcgtcgaca
      601 gcgtgctcca gtcggtccat ttgaccgcgc cgaaagccag ccagccgggc gcgatgccgg
      661 tctggaactt gatatcgacg ctttgcggca ggttgccgac gcctgtggcg gcaacgggaa
      721 cggccgcggt cgcaggcaag gcgaggaacg gattggcgcc attgggaacg ccctgccgcg
      781 ccaacgcgcc ggccagaacg ccggcaggcg cggtggcggt gccttgcgcg ccgtaatcgg
      841 tgcccgaacg gtacatgacc tgaccgcgca gggcgagttc ggggatttcg taggccgcgc
      901 cgatgcggta gccgaacttg cggccatcaa gactaagctg ggcattgccg aaggggccgt
      961 aggaattctc acggtaatag tcgagcgtct cgatatagcc gccgccgagc agccagaact
     1021 tgccctggcc ggcgtcgaaa ctgacgccgc aggtgcccgc cgcttcgtag gtggtgaatt
     1081 cttccatcgt cttgccgcta tatgtcggaa aatcgtagcg ggcggagcca ccattatttt
     1141 gcacataggt gccgacgcag cgaagattgt cggtaaggtt gaacttggcc gccgccgacg
     1201 ggaccatgta atcttcggtg tagctggtgc cgacgagggc cggattaggg ttcttcgaga
     1261 actcccggtg cgggctgaca taggtgacgc tggagcgcat gttgaagttg ccgtcctcat
     1321 agatgaggtc ggtatctgcg ctgccacgcg aaaaaccacc cgcataggcc gcaccgccca
     1381 tcgccaacga tagaaatgcc gtaccaagca gcgccttcac acgcgaattg cccatagatc
     1441 gttctccccg aaaatcaatg gtgcaagcta gaaccaaata cggttaccgg atcaacaacg
     1501 atttaccggg cgcagccccg gccgaccgcc ggacaccgtt gtggcggcag ttttacctcc
     1561 aaatagggac tggaaaatcg cgaaaagccg catttaccgg ccaagctcga gcgatatttt
     1621 gcggaaaatc gggaaaaatg gcacgaaaaa cagccaaatt ggccggtaaa agccggtttt
     1681 gttacattag ggcaacaatg cggcggaaac ttccgttaac gtcagaattc ggtattttga
     1741 acggcgtttc gagccggaaa tacccgaaat ccgggagcgg ccgcccgctc ccgaatctca
     1801 tggcggccat cgttcagccg gcgtcgcgca ccttgcccag cgccacggaa agcttttcct
     1861 gggtctcgcg gtattccgcc agcttctccc gctcggccgc gaccacgtca tcgggcgcat
     1921 tggcgacgaa cttgtcgttg gacagcttct tgtgcaggcg ggcaatctcg tcggtggcct
     1981 tggcgagttc cttttgcaaa cgggcggctt cagccgccag atcgatcagg ctgccgagcg
     2041 gcaggctgac ggtcgcctcg ttcaaaacga tctgcgcgga gcccttgggc gcggcgtcgg
     2101 ccagcgaaat ctcaccgatg cgcgccaggc gcttgattgc cggatcgtgg cgcgccagcc
     2161 gttcgcgcgt cgtatcgttg gcgccaacca cgaccagcgg cgcgatggca gccggcggca
     2221 cgttcatctc cgagcgcacc gagcggatgc ccgtcaccag gtcgatcagc cagttgatat
     2281 cgcttgccgc ttcctcgtcc tcgaaatcgg gagccggcca cgcggcatgg gccagcaacg
     2341 attgccgctg ctgcccctcg cccgccgtct gcgcccacaa ttcctccgtc atgaacggca
     2401 tcatcgggtg caaaagcttg tagatctcgt ccagcacgaa ggcgacgcag gcgcggcttt
     2461 cgaccttggc ctcctcgtcg tcgcccatga agaccggctt caacagctcc agataccaat
     2521 cgcaaaccat gttccagacg aagcggtagg cggcacctgc cgcctcattg aagcgatagg
     2581 aggtgatgcc ttcggtgatc gcgcgcgtcg cgcgggtcag ttcggtcaaa atccaacggt
     2641 tgaccgccag tttggcgtca cccagccaca aatcgtcgtt gcgcgccacc ccgttcatct
     2701 cggcaaagcg cgtggcgttc cacagcttgg tgccgaaatt ccggtagccg gcgatacggg
     2761 ccggatcgag cttcacgtcg cggccctgcg cggccatgct ggtcagcgtg aagcgcagcg
     2821 cgtccgcgcc atactcgtcg atcagttcca gcggatcgat gacgttgcct ttcgacttcg
     2881 acatcttggc gccgttcttg tcgcgcacca gcgcgtggac gtagaccgtg tggaacggct
     2941 cttcgtccat gaaatgcaag cccatcatca tcatgcgggc aacccagaag aagatgatgt
     3001 cgaagccggt caccagcacg tcggtctggt aataggtttt cagttccggc gtcttgtcgg
     3061 gccagcccaa tgtcgagaac ggccataggg cggaggaaaa ccacgtatcg agcacatctt
     3121 cgtcgcgggt gaggatttca cccggctgga agttttccag cctttcctcg acccatgcct
     3181 tccacggccc ctcatgggcg agataatgct ggatcgccgc atcaagggcg gcctcttcgg
     3241 tcttttcgac gaagaccgcg ccgtccggcc cgtaccatgc cggtatctga tggccccacc
     3301 agagctggcg cgaaatgcac caaggctgga tgttttccat ccagtcgaaa taagtctttt
     3361 cccagttttt cggcacgaag ctggtgcgcc cctcgcgcac cgaggcaatc gccggtttcg
     3421 ccagttcggc ggcgttgaca taccactggt cggtcaagaa cggctcgatc ggcacgccgc
     3481 cacggtcgcc atgcggcacc tggtggcggt ggggttcgat cttgtcgagg aagccgcctt
     3541 cctccatcag ctcgacgatg cgcttacggg ccgcgaaacg gtcaaggcca tgcaattcgt
     3601 tccagacggc ctgccgctcg ggcgttgagt caaggccggc gaggaagtcc tcattatcgt
     3661 tgatgtttat cgccgcctcg acggt
//
LOCUS       DASYCS010000410         4401 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4177666,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000410 DASYCS010000000
VERSION     DASYCS010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4401
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4177666"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VGM46_12680"
     CDS             <1..498
                     /locus_tag="VGM46_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY4193494.1"
                     /translation="ESVEDPDDYYEFLEQPPVEKVQRLYSVDEVKRSARVRDIARRID
                     LDTLTFEFGSASISDSEVQKLEGVATAMEKLLDKNPAETFLIEGHTDAVGTDNANLAL
                     SDRRAEAVAEALTNAFGIPPENLSTQGYGERYLKVNTTAPERKNRRVAIRRITSLVAP
                     VASAE"
     gene            716..1252
                     /locus_tag="VGM46_12685"
     CDS             716..1252
                     /locus_tag="VGM46_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018428827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HEY4193495.1"
                     /translation="MKRLELAVETIILATRWLLVVFYLGLGLALAVYAVSFGKKLVDF
                     VGNVSLLDETDTILKMLGLIDAALVASLVVMVIISGYENFVSRFDDDNGGVHWLGTID
                     VGSLKVKVASTIVAISSIHLLQIFLNSSSYTTTQLMWLTIMHLAFVVSALMLAYIDRV
                     MASGKSAKKGAADEAPGI"
     gene            complement(1370..2236)
                     /locus_tag="VGM46_12690"
     CDS             complement(1370..2236)
                     /locus_tag="VGM46_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006206158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY4193496.1"
                     /translation="MSLKGKTLFISGGSRGIGLAIALRAARDGANVTIAAKTAEPNPK
                     LPGTIYSAAAEIEEAGGKALPVVCDIRDEAQVAEAVAKTVEKFGGIDICINNASAIQL
                     TGTLETDMKRYDLMHQINTRGTFLVSKMCIPHLKLAQNPHILNLAPPLDMEAKWFKNH
                     VAYTMAKFGMSMCTLGMSAEFASDGIAVNSLWPITAIDTAAVRNLLGGASVAAMSRSP
                     EIMADAAHAILTRPSREATGNFYIDEEVLRAEGVSDFSKYAPGAKGPLVGDFFVPDAV
                     FARTDTEVKGMF"
     gene            complement(2389..2685)
                     /locus_tag="VGM46_12695"
     CDS             complement(2389..2685)
                     /locus_tag="VGM46_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyU family transcriptional regulator"
                     /protein_id="HEY4193497.1"
                     /translation="MSFLKRLFGGGSSGEAASPEPGAPVKQVEHKGFTIGATPFKADG
                     QYQTSGIVSKEIDGEVREHRFIRADRFPGLDDAIDTSIRKGIQIVNEQGERIFD"
     gene            complement(2788..3459)
                     /locus_tag="VGM46_12700"
     CDS             complement(2788..3459)
                     /locus_tag="VGM46_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008877540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEY4193498.1"
                     /translation="MHGHNNCGGRGERAFLHMAGRFGGRGGGGFGGFGGGMGRGGRGG
                     PGDMFRAGKMLADGDLKLIALALLAQTPRHGYDIIKALEEKSSGFYSPSPGVVYPTLT
                     FLEEAGYAASAPEGAKKVFSITEDGQKHLAENRELVERVLEQIERFGRKMAKAREWFG
                     WNDDGEEHGNRGQAAGKRDEFRAVRQRLRAALGEIADAPAEKQAEAIAILANAAEAIE
                     ALTRP"
     gene            complement(3642..>4401)
                     /gene="rpoD"
                     /locus_tag="VGM46_12705"
     CDS             complement(3642..>4401)
                     /gene="rpoD"
                     /locus_tag="VGM46_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019997940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEY4193499.1"
                     /translation="NREVSIAEAKARRAKKEMVEANLRLVISIAKKYTNRGLQFLDLI
                     QEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKI
                     VRTSRQMLHEIGREPTPEELAEKLAMPLEKVRKVLKIAKEPISLETPVGDEEDSHLGD
                     FIEDKMAILPIDAAIQANLRETTTRVLASLTPREERVLRMRFGIGMNTDHTLEEVGQQ
                     FSVTRERIRQIEAKALRKLKHPSRSRKLRSFLDS"
BASE COUNT          796 a         1410 c         1285 g          910 t
ORIGIN      
        1 gaatcggtcg aggacccgga cgactactat gagttcctcg aacaaccgcc cgtggaaaag
       61 gtgcagcgcc tctattcggt cgatgaggtg aagcgctcgg cccgcgtgcg cgacatcgcc
      121 cgccgcatcg atttggatac gctgaccttc gagttcggct cggcctcgat ctccgacagc
      181 gaggtgcaga agctggaagg cgtggccacc gcgatggaga agctgctgga caagaacccc
      241 gccgaaacct tcctcatcga gggtcacacc gatgcggtcg gcaccgacaa cgccaacctg
      301 gcgctatcgg accggcgcgc cgaggcggtg gccgaagcgc tgaccaacgc cttcggcatt
      361 ccgccggaaa acctgtcgac gcaaggctat ggcgaacgct acctcaaggt gaacaccacc
      421 gcgcccgagc ggaagaaccg gcgcgtcgcc atccgccgta tcacttcgct ggtggctccg
      481 gtcgccagcg ccgagtaact ttcggccggt cctgctgttt gataccggtc attgaacagc
      541 ggggctgccg cccccagatt aaggcatggg cgttccctgc cctgtcccgt ctccagcggg
      601 acgaaaggcc ccgccggtgc ccctcgccgg cggggttttt ttgagacggg agcatcccgg
      661 tccttgcaat gaaaacacgc ctgcccgcaa aatcggcggc cacgggagct tagaaatgaa
      721 acgcctggaa ctcgccgtcg aaacgatcat cctcgccacg cgatggctgc tggtcgtctt
      781 ctatctgggc cttggccttg cgctggcggt ctatgccgta tcgttcggca agaaactggt
      841 cgatttcgtt ggcaacgtct ccctgctcga cgaaaccgac accattttga aaatgctcgg
      901 cctcatcgac gccgccctgg tcgcctcgct ggtggtgatg gtcatcattt ccggctacga
      961 aaacttcgtc agccgcttcg acgacgacaa tggcggtgtg cactggctgg gcaccatcga
     1021 tgtcggctcg ctgaaggtga aggtcgcctc caccatcgtc gccatttcct cgatccacct
     1081 gcttcagata ttcctgaaca gctcgtccta taccaccacg cagttgatgt ggctgacgat
     1141 catgcatctg gccttcgtcg tgtcggcgct gatgctggcc tatatcgacc gcgtcatggc
     1201 ctccggaaaa tccgccaaga aaggcgcggc cgacgaggcg cccggcatct agagcggttc
     1261 tggttttacc ggatcggcaa aaccagaacc gctctatctt tttgttttgg ccgcatttcc
     1321 ggacgcaaaa ccgtttcaca cttttgctgt aaatgctcta ggccaagggc tagaacatac
     1381 ctttgacttc ggtgtcggtg cgcgcgaaca ccgcgtccgg cacgaagaaa tcgccaacca
     1441 gcggcccttt cgcgccgggc gcgtatttcg agaaatcgct gacgccctcg gcgcgcagca
     1501 cctcctcgtc gatgtagaaa ttgccggtcg cctcgcgcga cggcctggtc aggatcgcgt
     1561 gggcggcatc ggccatgatc tccggcgacc ggctcatcgc cgcaaccgat gccccgccca
     1621 gcaggttgcg caccgccgcc gtatcgatgg ccgtgatcgg ccatagcgaa ttgacggcga
     1681 tgccgtccga agcgaactcg gcgctcatgc cgagtgtaca catcgacatg ccgaacttgg
     1741 ccatcgtata ggcgacgtgg ttcttgaacc atttcgcttc catgtcgagc ggcggcgcca
     1801 gattcaagat atgaggattt tgcgccaact tcaggtgcgg aatgcacatt ttggaaacga
     1861 ggaacgtgcc gcgcgtattg atctggtgca tcagatcata gcgcttcatg tcggtttcca
     1921 gcgtgccggt aagctggatg gcgctggcat tgttgatgca gatatcgatg ccgccgaatt
     1981 tctcgacggt cttggcgacc gcttccgcca cctgtgcctc gtcgcggata tcgcagacta
     2041 ccggcagcgc cttgccgccg gcctcttcga tctccgccgc cgccgaatag atcgtgccag
     2101 gcagcttcgg gttcggctcg gccgtcttgg cggcgatggt gacattggcg ccgtcgcgcg
     2161 cggcgcgtag cgctatcgcc agcccgatgc cgcgcgaacc gccagagatg aacagcgtct
     2221 ttcccttgag cgacatggcg caacccttca ttggaaagaa gccagtgtgg ccttcggcaa
     2281 ccacggcagg caagcttttt tcggttgcaa cagacttacg ttagggatgg tgccgcgcgg
     2341 ccttgcctgg catgcccgcc gcgacggccc tcggtcaaaa tcctgctttc aatcgaaaat
     2401 ccgttcgccc tgttcattga cgatctggat gcccttgcgg atcgacgtgt cgatcgcatc
     2461 gtccagtccg gggaaacggt cggcgcggat gaagcggtgc tctctgactt caccgtcgat
     2521 ttccttcgag acgatgccgg aggtctggta ttgcccgtcc gccttgaacg gcgtcgcgcc
     2581 gatggtgaaa cccttgtgct cgacctgctt gaccggcgca cccggctccg gcgaggccgc
     2641 ctcgcccgac gatccgccac caaacagacg cttcaggaat gacatggcca gcctccaacg
     2701 aaaacatcct gcccgcgacg ttgcgggcag gatgttttca ctaaacgcca ttggacagcc
     2761 gggcaagggt taccccagcc aaagcattca gggacgggtt agcgcctcga tggcctcggc
     2821 ggcatttgcc aggatggcga tcgcctcggc ctgcttttcg gccggcgcat cggcgatctc
     2881 gccaagcgct gcgcgaagcc gctggcgaac ggcgcggaat tcgtcgcgtt tgcccgccgc
     2941 ttgtccgcgg ttgccatgct cctcgccatc gtcgttccag ccgaaccatt cgcgcgcctt
     3001 ggccatcttg cgcccgaagc gctcgatctg ctccagcact cgctcgacca gttcgcggtt
     3061 ttccgccaga tgtttctggc catcttccgt gatggaaaac accttcttgg ccccttccgg
     3121 cgccgatgcg gcatagcccg cctcttccaa gaaggtcagc gtcggataga ccacgccggg
     3181 gctggggctg tagaaaccgc tggatttctc ctccagcgcc ttgatgatat cgtagccgtg
     3241 gcgcggcgtc tgcgccagca gggccagcgc aatcagcttc aaatcgccat cggccagcat
     3301 cttgccggcg cgaaacatat cgcccggccc gccacgaccg ccacgcccca tgccaccgcc
     3361 aaacccgccg aatccaccgc cgccgcgccc gccgaagcgg ccggccatgt gaaggaaagc
     3421 gcgttcgccg cgcccaccgc aattattatg tccgtgcatt gatatctcct tgagcaatgt
     3481 cttacgatat atcttaagta ggctcacgca ttcgcagagt caagatatat cttaagatgc
     3541 atctggttga ggcgttaaag cggcgtcgcg tcatactgaa ataaaaaaag gcccggagat
     3601 cgctctccgg gccttttaaa aattcgggtc tggtgtttgg cttagctgtc gaggaacgac
     3661 cgcagcttgc ggctccggct cggatgcttg agcttgcgca gcgcctttgc ctcgatctgg
     3721 cggatacgtt cgcgcgtcac cgagaactgc tggccgactt cttccagcgt gtggtcggtg
     3781 ttcatgccga tgccgaaacg catgcgcagc acgcgctcct cgcgcggcgt cagcgaagcc
     3841 aaaacccgcg tcgtcgtctc gcgcagattg gcctggatcg cggcgtcgat tggcaggatc
     3901 gccatcttgt cttcgatgaa atcgccaaga tgcgaatcct cctcgtcgcc gaccggcgtt
     3961 tcgagcgaaa tcggctcctt ggcgatcttc agcaccttgc gaaccttctc gagcggcatg
     4021 gcgagcttct cggccagctc ttccggcgtc ggctcacggc caatctcgtg cagcatctgg
     4081 cgcgaggtgc gcacgatctt gttgatcgtc tcgatcatat gcaccggaat tcggatggtg
     4141 cgggcctggt cggcaatcga acgggtgatc gcctgcctga tccaccatgt ggcataggtg
     4201 gagaacttgt agccgcgacg atactcgaac ttgtcgaccg ccttcatcag gccgatgttg
     4261 ccttcctgaa tcaggtcgag aaattgcagt ccgcggttgg tgtacttctt ggcgattgaa
     4321 atcaccaggc gcaggttggc ctcgaccatt tccttcttgg cgcggcgagc cttggcttcg
     4381 gcaatcgaca cctcgcggtt g
//
LOCUS       DASYCS010000411         4844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3455430,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000411 DASYCS010000000
VERSION     DASYCS010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4844
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3455430"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="VGM46_12710"
     CDS             complement(<1..719)
                     /locus_tag="VGM46_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEY4193500.1"
                     /translation="MDLEKYSERVRGFIQSAQTMALSRNHQQFTPEHILKVLVDDDEG
                     LAASLIERGGGNARDVRLGVETALEAMPKVEGGNGQLYLAQPLAKVFSTAEDLAKKAG
                     DSFVTVERLLQALAMEKSAKTADILAKAGVTAQALNQVINDLRKGRTADSASAEQGYD
                     ALKKYARDLTADARAGKLDPVIGRDDEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIAE
                     GLALRIVNGDVPESLKDKQLMA"
     gene            complement(944..1822)
                     /locus_tag="VGM46_12715"
     CDS             complement(944..1822)
                     /locus_tag="VGM46_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4167 domain-containing protein"
                     /protein_id="HEY4193501.1"
                     /translation="MRPQQQNRRMRGRNNNGGGGNNNNNNNNRKGPNPLTRNYESNGP
                     DVKIRGSAQQIAEKYGTLARDAHSSGDRVMAENYLQHAEHYNRIIAAAQAQMPIQNTQ
                     NNRDDFDEDGEEDRDDFDNGGNGNLADTGGSQANNGSGPQPVIEGTPAEIALNAEGGR
                     ETGGREGSGRRERRPNGYGQNGQRGDNPSRGEQNSQRSEQSRRNEAPVSSEASEIDAA
                     APEIIAAPVAQTEHLSPADLAARAELNEAANEAAAPRRPRRPRRPRVSADEAGGEGSV
                     EAPGDAKAVEPATGES"
     gene            complement(2127..3026)
                     /gene="prmC"
                     /locus_tag="VGM46_12720"
     CDS             complement(2127..3026)
                     /gene="prmC"
                     /locus_tag="VGM46_12720"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEY4193502.1"
                     /translation="MNSPSAANLPTTLGQLHREAKLRLAQAGVETPSLDARLLVEHFS
                     GTTRTQAISEPERPVDASAVATIVDALARRLAGEPVHRILGFREFYGLRLSLSPGTLE
                     PRPDTETLVEAALARVKSVTARKGGCRILDLGTGTGAIALALLSEVAGAVATGVDVSE
                     DALATAARNAGELGLSGRFTLLKSDWFSRVSGQYDVIVSNPPYIGTNDIATLQAEVRE
                     YDPLRALDGGEDGLLAYRLIAEGAKAFLAPDGYIAVEIGATQRADVTGIFIEAGLALV
                     EARRDLGGNDRVLAFNAASGAQR"
     gene            complement(3041..4117)
                     /gene="prfA"
                     /locus_tag="VGM46_12725"
     CDS             complement(3041..4117)
                     /gene="prfA"
                     /locus_tag="VGM46_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEY4193503.1"
                     /translation="MTNLPNDRMDQVVKRFDMLEAQMAAGPAPDAYVKMASEYADIQD
                     LAGKIRTLRAAEGEQRDLEAMLADKNTDAEMRGLAEADLPGVKERIETLQQDIQILLL
                     PKDAADEKNAILEIRAGTGGDEAALFAGDLFRMYERYAAERGWRFETASASDGDAGGY
                     KEIIATVSGKGVFAHLKFESGVHRVQRVPTTEAQGRIHTSAATVAVLPEAEEVDIDIK
                     ADDIRIDTMRASGSGGQHVNTTDSAVRITHLPTGIMVVQAEKSQHQNRARAMQILRAR
                     LYDLERSRADEERSSSRKSQVGSGDRSERIRTYNFPQGRVTDHRINLTLYKLDRVMMG
                     ELDEIVLALIADHQSKLLADISEN"
     gene            complement(4114..>4844)
                     /locus_tag="VGM46_12730"
     CDS             complement(4114..>4844)
                     /locus_tag="VGM46_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008507649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HEY4193504.1"
                     /translation="GGDKVLPYFKGSLQEENPALGWRAIRLTLDRPGLLRTQIRALLR
                     ACGGRELKLMLPMVTELGEIAQAREIIDREVRHLSRFAHHLPTSLKLGAMLEVPSLLF
                     QLDELMKAVDFVSVGSNDLFQFVMATDRGNTQLADRFDPLSVPFLRVLKRIVDAGNRN
                     LTPVTLCGELAGKPISAMALIGLGYRSISMSAASIGPVKAMLTELPLEELKRFFDDNL
                     SAPATGMPMRALLQAFADDRSIPL"
BASE COUNT          818 a         1596 c         1457 g          973 t
ORIGIN      
        1 gccatcagct gcttgtcctt cagcgattcc ggcacatcgc cattgacgat gcgcaacgcc
       61 aggccttcgg cgatggccgt cttgccgacg cccggctcgc cgatcagcac cggattgttc
      121 ttggtgcgcc gcgacagcac ctggatggtg cggcgtatct cgtcgtcgcg gccgatgacc
      181 ggatcgagct tgcccgcgcg cgcatccgcc gtcaggtctc gcgcatattt cttcagcgca
      241 tcgtagccct gttcggccga ggccgaatcg gcggtgcggc ccttgcgcaa atcattgatg
      301 acctggttca gcgcctgcgc ggtaacgccg gccttggcca gtatgtcagc cgtcttggcc
      361 gacttttcca tggcgagcgc ctgcaaaagc cgctccacgg tgacaaacga atcgcccgcc
      421 ttcttggcca gatcctcggc ggtcgaaaac actttggcca gcggctgcgc cagatagagc
      481 tggccgttgc cgccctccac tttcggcatg gcttccagcg ccgtttcgac gccaagcctg
      541 acgtcgcgcg cattgccgcc cccacgctct atcagcgatg cggccaggcc ctcatcatcg
      601 tcgacgagca ccttcaatat atgttcgggg gtgaattgct gatggttgcg cgacaacgcc
      661 atcgtctgcg cggactggat gaagccgcgc acgcgctccg aatatttctc aagatccatg
      721 gatagtctcc cttccatcac cggcccgctt tgcggcaccg gatccgactg cggtgaggaa
      781 cccgccagct tggccgggtc cagttgcgat agatatggtg cacggcttgc cgtccctcaa
      841 gatgccggga acggcgcatc gcaaaaaagg gcggcttaaa acaaaagcag cggaggatgt
      901 cccccgccgc ttgcattgct caagatgtca ggccgatcga cggtcagctt tcgccggtcg
      961 ccggctccac ggccttcgcg tcacccggcg cttccaccga accctcgcca ccagcttcgt
     1021 ctgcgctgac gcgcggacgg cgtggccgtc gcggacggcg cggggctgcc gcctcgttag
     1081 ccgcttcgtt cagttcggcg cgcgcggcaa gatcggccgg cgaaagatgt tcggtctgcg
     1141 ctacaggcgc cgcgatgatt tcgggcgccg cagcgtcgat ctcggaagcc tcggacgata
     1201 cgggcgcctc gttgcgccgg ctttgctcgc tgcgctggct gttctgctca ccgcgcgacg
     1261 gattatcgcc gcgctggccg ttctggccgt agccattggg gcggcgctcg cgacggccgc
     1321 ttccttcgcg gccacccgtc tcacggccac cttctgcatt gagggcgatt tcggctggtg
     1381 tgccttctat gaccggctgc ggccccgaac cattattggc ttgcgaaccg ccggtatcgg
     1441 caagattgcc attgccgcca ttgtcgaaat cgtcgcgatc ctcttcgccg tcttcgtcaa
     1501 agtcgtcgcg attgttctgg gtgttctgga taggcatctg cgcctgcgcc gccgcgatga
     1561 tgcggttata gtgctcggcg tgctggagat agttctccgc catgacgcgg tcgccggaac
     1621 tgtgcgcatc gcgcgccagt gtcccgtatt tctccgcgat ctgctgggcc gaaccgcgaa
     1681 tctttacgtc cgggccattg ctctcgtaat tgcgggtaag gggattgggc cccttgcggt
     1741 tgttattgtt gttattattg ttgccgccgc cgccattgtt gttgcgaccg cgcatgcgcc
     1801 tgttctgctg ttgtggcctc attagactct cttcatcatg gaatttgaaa actctttgcc
     1861 aacggcggcg ctcatgcgtc ctgccgtttc ggtgtctcac cggtgctcgc ccgcatcgat
     1921 tgcgtcggcg tcacgtgcca tcctgctcgg ttacatatca cttgccggac gattcccgct
     1981 cgaagctcgg cgtcgattgc gcaaaagggc agatgtttcc aaggaaaccg aatcgctgga
     2041 gcactgcctg cttcgtctcg tccggttaca aaaaccctag ccgcattcaa aagccttgcc
     2101 aagcgttttt tcaaaaccgg ctcaggctat ctttgcgccc cggacgcggc attaaacgcc
     2161 agaacacggt cgtttccgcc aaggtcgcgg cgcgcttcaa ccagggccaa tcctgcctcg
     2221 atgaatatgc cggtgacatc cgcccgctgc gtcgcgccta tctcgaccgc gatatacccg
     2281 tccggcgcga ggaatgcctt cgccccttcg gctatgagac ggtaagccag aagtccgtct
     2341 tctccgccat caagcgcgcg aagggggtcg tattcccgca cttcggcctg caaagtcgct
     2401 atgtcgttgg tgccgatata aggcggattg gagacaatta catcatattg gcccgaaacc
     2461 cgcgaaaacc agtccgattt gagcagcgtg aaccgcccgg acaggccaag ctcgccggca
     2521 ttgcgcgccg cggtcgccag cgcatcttcg gaaacatcga cgccggtcgc aaccgcgccg
     2581 gcgacctcgc ttagaagcgc cagcgctatc gcgccggttc cggttccaag gtcgaggatg
     2641 cggcatccac ccttgcgcgc cgtcaccgat ttcacgcgcg ccagcgccgc ttcaaccagc
     2701 gtttccgtat cggggcgggg ttcaagcgtg ccgggcgaca gcgaaaggcg cagcccgtag
     2761 aattcgcgaa acccgaggat gcgatgcacc ggctcgcccg ctagcctgcg cgccagcgca
     2821 tcgacgatcg tggctaccgc cgacgcatcg accggccgtt caggctcgga aatggcctgc
     2881 gtccgtgtcg taccggaaaa atgctccacc aaaaggcgcg catcgagcga cggcgtttcg
     2941 acgccggcct gcgccagccg aagctttgcc tcgcggtgca gttggccaag cgttgtcggc
     3001 aggttggccg cgctgggcga attcatcgaa ccgccaagcc tcagttttcc gaaatatcgg
     3061 caagcagctt cgactggtgg tcggcaatca gcgccaagac gatttcgtcc agttcgccca
     3121 tcatcacccg gtccagcttg tacagcgtca ggttgatgcg gtggtcggtg acgcggcctt
     3181 gcggaaaatt ataggtgcgg atgcgctccg aacggtcacc cgacccgacc tgcgacttgc
     3241 gcgacgaaga acgctcctcg tcggcgcggc tgcgttcaag gtcatagagc cttgcccgca
     3301 ggatttgcat ggcgcgcgcg cggttctggt gctgcgattt ttccgcctgc accaccataa
     3361 tgccggtcgg cagatgggtg atgcgcaccg ccgaatcggt ggtgttgacg tgctggccgc
     3421 cggaaccgga agcccgcatc gtatcgatgc ggatatcatc ggccttgatg tcgatatcga
     3481 cttcctcggc ttccggcagc accgccaccg tcgccgccga agtatggatg cgcccctgcg
     3541 cctcggtggt cggaacccgc tgcacccggt gcacgccgga ctcgaacttc agatgcgcga
     3601 acacgccctt gcccgaaacg gtggcgatga tttccttgta gccgccggca tcgccgtcgc
     3661 tggccgatgc cgtctcgaaa cgccagccgc gctcggctgc atagcgctca tacatgcgaa
     3721 acagatcgcc cgcgaacagc gccgcctcgt cgccgccggt tccggcccga atttcgagga
     3781 tggcgttttt ctcgtctgcg gcgtccttgg gcaaaagcag gatttgtatg tcctgctgca
     3841 atgtctcgat gcgttccttg acgcccggca aatccgcctc ggccaggccc cgcatttcgg
     3901 catcggtgtt cttgtcggcc agcatcgcct caaggtcgcg ctgctcgcct tcggcggcgc
     3961 gcagcgtcct aatcttgccg gccagatcct ggatgtcggc atattccgac gccatcttca
     4021 cataggcgtc cggcgccggc ccggccgcca tctgtgcttc gagcatgtcg aagcgcttga
     4081 cgacttggtc catgcggtcg ttgggaagat tggtcataga gggattgaac gatcgtcggc
     4141 aaaggcctgc aacaaggccc gcatcggcat gccggtggcc ggcgcggaaa gattgtcgtc
     4201 gaaaaaccgc ttgagttctt ccagcggcaa ttcggtcagc atcgccttga ccgggccgat
     4261 cgaggccgcc gacatcgaga tcgagcggta gccaagcccg atcagcgcca tcgccgaaat
     4321 cggcttgccc gccagttcac cacacagggt caccggggtc aggttgcgat tgccggcgtc
     4381 gacgatcctc ttcagcacac gcaggaacgg caccgacagc gggtcgaaac ggtccgccag
     4441 ttgcgtattg ccgcgatccg tcgccatgac gaattggaac agatcgttgg aaccgacgga
     4501 aacgaagtcc accgccttca tcaactcatc gagctggaac agcagcgacg gcacttccag
     4561 catcgcgcca agcttgaggc tggtcggcag atggtgggcg aaacgcgaca ggtggcgcac
     4621 ctcgcggtcg atgatttcgc gcgcctgcgc aatttcgccg agttcggtga ccatcggcag
     4681 catcagcttc agctcgcgcc cgccgcatgc ccgcaaaagc gcgcgaatct gggtgcgcag
     4741 caggcccggc cggtcgagcg tcagccggat cgcgcgccag ccgagcgccg ggttttcttc
     4801 ctgcaacgat cccttgaaat agggcagcac cttgtcgcca ccga
//
LOCUS       DASYCS010000412         4408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3166170,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000412 DASYCS010000000
VERSION     DASYCS010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4408
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3166170"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(292..744)
                     /locus_tag="VGM46_12735"
     CDS             complement(292..744)
                     /locus_tag="VGM46_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018426441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY4193505.1"
                     /translation="MTGATQAANTNDSQGDCGDDLARIGEMAKSFGVTLRTLRFYEDK
                     GLLNPKREGSTRLYTRRDRARLKLILLGRKIGFSLRDVKQMIDLYDPTGSNARQLKLT
                     LEKSEKQFVRLQKQRMVIDEAIAELEGTMNSVRAMLVDRAAEKNRAAG"
     gene            1207..>4408
                     /locus_tag="VGM46_12740"
     CDS             1207..>4408
                     /locus_tag="VGM46_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HEY4193506.1"
                     /translation="MNSKRSYLDTPNAGRQRQPLSTLDQLNQSLQALEQQIGRTRGAP
                     AEPRYPGTQPYAQRPAEQPRAYPQARASMQAAPRYEQNYQAIARDIDRARGQEDSVAM
                     IGKIAGELRGLREELRHQTATGLHHEFEALRADIDRACQTAPASRQTEELGREFERLS
                     GAIQMLAEKSDDRSVNSLRLEMEQVRSALDALAREESVQSVDRRFDDFDRRWSAFETR
                     HDADQRSRSDGPGIAMLAERLEQIGSAVNNLPESLSLRSLEEKVRTLAAALDHFTSRE
                     NSRSGDTLAMIDERLDEISRAIVASTVAAQASGLDAEAFERIENRISALAAQIEEVAG
                     DRPDGEVIDRLNMLSSRVDQLATRANLPEQAMERLGQQIALIADKIDHAPSMPDPDYI
                     FQGLEQRFEVLSSMMERRQGDALEQGNMLFRDLERRLDEVADRLDQRMPGAELDSAGI
                     MDAIDARFTALAKRLETRAPDPAGAAAIRGLESRLEDISARLDHSAAQVASIDPQLIR
                     SLEAQVTGLSAHLTQPGTPLPEFEDIVPRLNEIERSLAGTHDSIVSAAREAAESAVRS
                     LAGSSANTAAVSGLAEDLKTLETLTRRSDERNSKTFEAIHDTLLKIVERLGSLEHSEP
                     ASAAKIAVQNAPSMDFGQSLAAETIGSAQNMEQAESAGAPGSMAAVRAGEPLQRSPAQ
                     AAAAAALAALGADAIADKPEKPGRRKSMFGGLARAFSKKEAEAPALPGSAPVVDAPAV
                     DLDEPLDPRQANRPLEPGSGAPDLNAIMRRVRDERGQPARQGESEASNSDFIAAARRA
                     AQAAAAEADALKRQSAITGPVRALRVGDMLKVRRKPILMATTAIALALAGTLLGKAFL
                     SDAQRTADIGTTPIVAEQKMDADAISPAPAAPMPVSAPMSAQAAPADVQEAPSSDPVR
                     VVTPSAPKMAEGVVMPSNMPQDAETPMDDIGASPATAPAAPVAAAVSPAPATPTPATP
                     AATAPAATASDTTGTVAAQPMPEPAQAALDIPSDAGPAALRKAAAEGDAKALFEVASR
                     YAEARGVKQDMAAAAKWYEKSAELGLAPAEYRIGNFY"
BASE COUNT          826 a         1505 c         1389 g          688 t
ORIGIN      
        1 agcccagcat ttcattcagc acgaaaagcg tgtcgcgcac cggcgccgtg tagatcggca
       61 tatcttcctc caaccttgtg gcgcgagagc cagcgtgaat ggaacggtat cgtgaataga
      121 atgggcgccg ctgtcgagcc ggacctgaca gatccagacc tagcaaatat tgacgttcgc
      181 gtaaacgtca atatttgggt gggctttaat tttcagctac gcggtcgcgc ctgaccggaa
      241 tggcctggcg aatggcccag cgaacggaaa ccggaaatgg aagatgcgcg gtcagcccgc
      301 cgcgcggttc ttttccgccg cgcggtccac cagcatggcg cgcacggaat tcatcgtgcc
      361 ttcgagttcg gcgatggcct cgtcgatcac catccgctgc ttttgcaggc ggacaaactg
      421 cttctcggat ttttcaagcg tcagcttgag ctgccgggcg ttggagccgg tcggatcgta
      481 aaggtcgatc atctgcttta cgtcgcgcag cgagaacccg atcttgcgcc caagcaggat
      541 aagcttcagc ctggccctgt cgcggcgcgt gtagaggcgc gtcgagcctt cccgcttcgg
      601 gttgagcagg cctttgtcct catagaagcg caaggtgcgc agggtcacgc caaagctctt
      661 cgccatctcg ccgatacggg caagatcgtc accgcaatcg ccttgcgaat cgtttgtatt
      721 ggccgcctgc gtggcgcctg tcatcggttc catggttatt gccctcaaaa atgcaaagat
      781 gcggctgagg ccgcatcgac tgaccggaag caggcatgcc gcgcttccgg atcgtggttt
      841 tcaaaaatcg tgcagggccg tccgccgatg gccttacctt tacgtaagcg atacaaaacc
      901 ggaccggtac aggcaagggg ggccgtatgg gccagcagga acgcgcccgg cagcgtttgg
      961 cgggcctgtt tctgaacaaa gcctgaaact tattaagcat ggttaacgcg ttgtttacca
     1021 tgtcagggga aaggtaggcg cgtcggtcat ccgtggtcat cctgtatcga atttttcacg
     1081 gtcttcgaag tgtcgcgcaa aaccgggaag ccagtcttcc accccggccg aacgttccgc
     1141 ttcgattgcg acaggagttg agaggaaact ggccataagc cagccatttc gaaagacggc
     1201 gccccgatga acagcaagcg ttcatacctc gatacaccga atgccggcag gcagcgccag
     1261 ccgttgagca cgctggacca gctcaaccaa tcgctgcaag cgcttgagca gcagatcggc
     1321 cgcacgcgcg gcgcgcccgc cgaaccgcgc tatcccggca cccagcctta cgcccagcgg
     1381 ccggcggaac agccgcgcgc ttacccgcag gcgcgggcat ccatgcaggc agcgccccgc
     1441 tacgaacaga attaccaggc gatcgcccgc gatatagacc gcgcccgcgg ccaggaagac
     1501 agcgtcgcca tgatcggcaa gatcgccggc gaattgcgcg gcctgcgcga ggaattgcgc
     1561 catcagacgg ccaccggcct ccaccatgaa ttcgaagcgt tgcgcgccga tatcgatcgc
     1621 gcctgccaga cagcgcccgc ctccaggcaa accgaggaac tcggccgcga gttcgagcgg
     1681 ctttcgggcg cgatccagat gctggcggaa aaaagcgacg atagaagcgt caactcgctc
     1741 cggcttgaga tggaacaggt caggtcggcg ctcgacgcgc tggcgcgaga ggaaagcgtg
     1801 caatcggtcg atcgccgctt cgacgatttc gaccgccgct ggagcgcctt cgaaacgcgc
     1861 cacgatgccg accagcgcag ccgcagcgac ggtccgggca tcgccatgct ggccgagcgt
     1921 ctggaacaga tcggcagcgc cgtcaacaac ctgccggaat cgctgtcgct tcgctcgctc
     1981 gaagaaaaag tccgcacgct ggccgccgcg ctcgaccatt tcaccagccg ggagaatagc
     2041 cgttcgggcg atacgctggc gatgatcgac gagcggctgg acgagatttc gcgcgccatc
     2101 gtcgcttcga ccgtcgccgc acaggccagc ggcctggacg ccgaagcctt cgagcgcatc
     2161 gaaaaccgca tcagcgccct tgcggcccag atcgaggagg tcgccggcga ccgcccggac
     2221 ggcgaggtga tcgaccggct caacatgctt tccagccgcg tcgaccagct cgccacgcgc
     2281 gccaacctgc cggaacaggc gatggaacgg cttggccagc agatcgcgct gattgccgac
     2341 aagatcgacc acgccccttc catgcccgat cccgactata tcttccaggg gctggagcag
     2401 cgcttcgagg tgctttccag catgatggaa cgtcgccagg gcgatgcgct ggaacagggc
     2461 aacatgctgt ttcgcgacct cgaacggcgg ctggacgaag ttgccgacag gctcgaccag
     2521 cgcatgccgg gcgcggagct ggacagcgcc ggcatcatgg acgccatcga tgcgcgcttc
     2581 accgccttgg caaagcggct cgaaacccgc gcgcccgatc cggccggcgc cgccgccatt
     2641 cgcggcctgg aaagccggct cgaagacatt tcggcgcggc tcgatcattc agccgcgcag
     2701 gtcgccagca tcgatccgca actgatccgc agccttgagg cccaggttac cggcctttcc
     2761 gcccacctta cccagcccgg aacgccgctg ccggagttcg aggacatcgt tccgcgcctc
     2821 aacgagatcg agcgctcgct ggccggcacg catgattcca tcgtctcggc ggcgcgcgag
     2881 gcggcggaga gcgcggtgcg ctcgcttgcc gggtcgagcg ccaacacggc cgccgtttcc
     2941 ggtctggcgg aagacctcaa gacgcttgaa acgctgaccc gccgctccga cgagcgcaat
     3001 tcgaagacct tcgaggcgat ccacgacacg ctgctgaaga tcgtcgagcg ccttggctcg
     3061 ctggaacaca gcgagccggc gtccgccgcc aagatcgccg ttcaaaacgc gccgtcgatg
     3121 gatttcggcc agtcgcttgc ggccgagacc atcggttcgg cgcaaaatat ggaacaggcc
     3181 gaaagcgcgg gcgcgccggg ttcgatggcc gccgttcgcg ccggcgaacc cttgcagcgc
     3241 tcaccggcac aggccgcagc cgcggcagcc cttgccgcac ttggcgccga tgccatcgcc
     3301 gataagccgg aaaagcccgg ccgcaggaag tcgatgttcg gcggtcttgc ccgtgccttc
     3361 agcaagaaag aagccgaagc cccggcgctt cccggttctg cccctgtcgt ggatgcgccc
     3421 gccgtcgatc tggatgagcc gctcgatccc aggcaggcca accgcccgct ggaacccggt
     3481 tcgggcgccc cggacctgaa cgccatcatg cgccgcgtcc gcgacgagcg cggccagcca
     3541 gccaggcagg gcgaaagcga agcgtccaat tccgacttca tcgccgccgc gcggcgcgcc
     3601 gcgcaggccg ccgccgccga agcggatgcg ctcaagcgtc agtcggcgat caccgggccg
     3661 gtgcgggcgc tgcgcgtcgg cgacatgctc aaggtgcgcc gcaagccgat cctgatggcg
     3721 acgacggcaa tcgccctggc gctggccggc acgcttctgg gcaaggcctt cctttccgac
     3781 gcccagcgca ccgccgatat cggcacgacg ccgatcgttg ccgagcagaa gatggatgcc
     3841 gacgcgatca gccccgcgcc tgccgcaccg atgcctgtat ctgcaccaat gtccgcgcag
     3901 gccgcgccgg ctgacgtgca ggaagcgccg tcaagcgatc cggtgcgcgt tgtcaccccg
     3961 tccgcgccca aaatggccga gggcgtcgtg atgccttcga acatgccgca ggacgccgaa
     4021 acgccgatgg acgacattgg cgcttcgccc gccaccgctc ccgcagcgcc ggtcgccgct
     4081 gccgtctcgc cagcgcccgc cacgccgacg cctgccacgc ccgcagccac ggcgccggcg
     4141 gcgacagctt ccgatacgac cggcaccgtc gccgctcagc cgatgccgga gccggcgcag
     4201 gccgcgctcg acattccatc ggatgccggc ccggccgcgc tgcgcaaggc cgccgccgaa
     4261 ggcgacgcca aggcgctgtt cgaggtcgcg tcgcgttatg cagaggcgcg cggcgtcaag
     4321 caggacatgg cggccgccgc caaatggtat gaaaaatccg ccgaactcgg ccttgcgccg
     4381 gcggaatacc gcatcggcaa tttctacg
//
LOCUS       DASYCS010000413         1768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4471188,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000413 DASYCS010000000
VERSION     DASYCS010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4471188"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="VGM46_12745"
     CDS             complement(<1..893)
                     /locus_tag="VGM46_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019996804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEY4193507.1"
                     /translation="MRLFEMNGADLLCNTLLANDIDVCFANPGTSEMHFVAALDRQPR
                     MRCVLGLFEGVVTGAADGYARMADKPAATLLHLGPGLANGLANLHNAKRAKSQIVNIV
                     GDHATYHLKYDAPLTSDIESLARPMSDWVGRAETADMVGVRTQEAIRAARRQPGHIST
                     LMLHADAAWTETAAPTPSPAETEKLPLADAVRIKEAALALRTGRNTMIVISGLGLREK
                     QLELLDRIAQKTNARLLAQQSNGRLQRGAGRIRLDRVPYVIDKALEVFADTEQVLLVG
                     AKSPVGFFAYPGKPSSMLPET"
     gene            complement(970..>1768)
                     /locus_tag="VGM46_12750"
     CDS             complement(970..>1768)
                     /locus_tag="VGM46_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEY4193508.1"
                     /translation="GMPGYDAAIQASCGIMSVNGEAGGQPLRVGLPVVDMVTGLNAAL
                     GILMALNERSVSGKGQFVEAALYDCGVSLLHPHLPNYYLSGKVAAPSGNAHPNICPYD
                     TFATADIPIFLAVGNNRQFVTLCEIIGRPDLPGDARFTNNGERNANRDALKVELEKAL
                     AAFNCHELSARLMKAGVPNGAVRTIDQVVADPHTKHREMVVEIGSYRGTGNPIKMSRT
                     PASYRLAPPHFAQHTGAILKEVSVDGESFREVLPGFEPEGPKLAEPA"
BASE COUNT          313 a          535 c          578 g          342 t
ORIGIN      
        1 catgtttcgg gaagcatgct gcttggtttg cccggatacg caaaaaagcc gaccggtgat
       61 ttcgcaccga ccagcaacac ctgctcggtg tcggcaaaca cctcaagcgc cttgtcgatc
      121 acgtaaggga cgcggtcgag cctgatgcgg ccggccccgc gttgcaggcg gccgttcgac
      181 tgctgcgcca gcaggcgcgc attggtcttc tgcgcgatgc ggtcgagcag ttccagttgc
      241 ttttcgcgca agccaagccc gctgatcacg atcatcgtgt tgcggccggt ccgcaaagcg
      301 agcgccgctt ccttgatacg aacggcgtcg gccagtggca gcttttcggt ctcggcgggg
      361 gagggtgtcg gggcagccgt ttcggtccat gcggcatcgg catgaagcat cagcgtcgag
      421 atatggcccg gctgacggcg cgcggcgcgg atggcctcct gcgtgcgaac gccgaccatg
      481 tcggcggttt cggcccgccc gacccagtcg gacatcggac gcgccagcga ttcgatgtcg
      541 gaggtcagcg gcgcgtcata tttcagatga taggtcgcat ggtcgccgac gatgttgacg
      601 atctgggact ttgcacgttt ggcgttgtgc agattggcca aaccgttcgc caggccgggg
      661 ccgagatgca gcagggtcgc ggccggcttg tcggccatgc gcgcataacc gtccgccgcg
      721 ccggtgacca caccctcgaa aagcccaagc acgcaccgca tgcggggctg gcggtccagc
      781 gccgccacga aatgcatttc ggaagttccc ggattggcga aacagacgtc gatgtcattg
      841 gccagcaggg tattgcagag tagatcggca ccgttcattt cgaacaatct catggtcaat
      901 tcgtgttaac aggatggtcc gccggccaca caaggaacat gccggcatca gccggcggcg
      961 cggggtcgct caggcaggtt ccgccagttt cggcccctcg ggttcgaagc cgggcaatac
     1021 ttcccggaag ctttcgccat cgacggaaac ctctttcagt atcgccccgg tatgctgggc
     1081 gaagtgaggc ggagccaggc ggtaggaagc cggggtcctc gacatcttga tcgggttgcc
     1141 ggtgccacgg taggagccga tttcaacgac catttcgcgg tgcttggtat gcggatcggc
     1201 aacgacctgg tcgatggtcc tgaccgcgcc gttgggaacg cccgccttca tcagccgcgc
     1261 cgatagttcg tggcaattga aggcggccag ggctttctcc aattccactt tcagcgcgtc
     1321 gcggttggcg tttcgctcac cattattagt gaagcgcgcg tcgcccggaa gatcggggcg
     1381 tccgatgatt tcgcacagcg tgacaaactg gcggttgttt cccaccgcga gaaagatcgg
     1441 aatgtcggct gtcgcaaacg tgtcgtaggg gcagatgttc ggatgggcgt ttccgcttgg
     1501 cgccgcgact ttgccggaca ggtaatagtt tggaaggtgg ggatgcagca gcgacacgcc
     1561 gcaatcgtaa agtgcggcct cgacgaactg tcccttgccg ctgacgctgc gctcgttcaa
     1621 ggccatcagt atgcccagcg cggcattcag gccggtgacc atgtccacca ccggcaagcc
     1681 tacgcgcagc ggttggccgc cggcctcgcc attgacgctc atgatgccgc aagaagcctg
     1741 gatcgcggcg tcatagcccg gcatgccg
//
LOCUS       DASYCS010000414         2073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3889787,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000414 DASYCS010000000
VERSION     DASYCS010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3889787"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /locus_tag="VGM46_12755"
     CDS             <1..1005
                     /locus_tag="VGM46_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEY4193509.1"
                     /translation="TVGIGECTVAPDQGGTAAILSRLASHLVGHSPYDGVPLISRIFH
                     QEYLGHGANIMRAANQMFSGIDMALWDLQGKLAKLPVHRLFGGAHRKAVGYFYFLQGE
                     TAEELAKDAAAGYASGERVFYLKVGRGEKLDLEITEAVRREIGGSRLRLDANEAWSVH
                     DAINMCRKLERFDIEFIEQPTVSWSIPAMAHVREKVGIPIVADQAAFTLYDVYEICRQ
                     RAADMICIGPREIGGLQPMLKAAAVAEAAGLKICIHSSFTTGITTCAEHHIALAIPNL
                     DDGNQIMWQLVRKDIVSSPDLAPKHGWIDAFQGPGLGFELNEDVIAEGERGFKASKAA
                     "
     gene            1002..2009
                     /locus_tag="VGM46_12760"
     CDS             1002..2009
                     /locus_tag="VGM46_12760"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEY4193510.1"
                     /translation="MTRPPARIPAGDAEKTAIAALMRSGALPENALPVARAMVRAEIE
                     GNAVCGLYYLPIFCAHLGCGKVDGKAKPQVVPDQAAIKVDARHGFAHPAIEAGLPHLI
                     DAARTFGIAALSIRNSYNCLALSHHVRPLAKEGLIGLSMANAPASVAPPGATRAIFGT
                     NPIAFAAPMADGDMIVVDQSMSAVTKTEMAMRRDRGQPIPTGWAQDRSGAPTTDAGEG
                     LRGSLLPSGGQKGANLALMIEILATALTGAELSSQASMFGDNQGGPPGVGQFMLAIDP
                     ARFSAGFAESLGALAESFQAAGVRLPGSGATAGKQDRDSILIDVDADLWQRCLDLAQG
                     R"
BASE COUNT          401 a          647 c          675 g          350 t
ORIGIN      
        1 acggtcggca tcggtgaatg cacggtggcg cccgatcaag gtgggacggc cgccatcctt
       61 tcgcggctgg cttcacatct ggtcggacat tcgccttatg acggcgtgcc tttgatctcc
      121 agaatattcc atcaggaata tctcggccac ggcgccaaca tcatgcgcgc ggccaaccag
      181 atgttttcgg gcatcgacat ggcgctgtgg gatttgcagg gaaagctcgc caagcttccg
      241 gtccaccgcc tgttcggcgg cgcgcaccgc aaagcggtcg gctatttcta tttcctgcaa
      301 ggcgaaaccg ccgaagaact ggcgaaggac gcggccgccg gttatgccag cggcgagcgg
      361 gtgttttacc tgaaggtcgg gcgcggcgag aaactcgacc tcgaaatcac cgaggccgtg
      421 cggcgtgaaa tcggcggatc gcggctccgg ctcgacgcca acgaggcgtg gagcgtgcat
      481 gacgccatta atatgtgccg caagctggag cgcttcgata tcgaattcat cgagcagccg
      541 accgtcagct ggagcattcc ggcaatggcc catgtgcgcg agaaggtcgg cattccgatc
      601 gtcgccgacc aagcggcctt caccctttac gatgtctatg aaatttgccg ccagcgcgcc
      661 gccgacatga tctgcatcgg cccgcgcgag atcggcgggc tgcaaccaat gctgaaagcc
      721 gccgccgtcg cggaagcggc cgggctgaag atctgcatcc attcttcctt cacgaccggc
      781 atcaccacct gcgccgagca ccatatcgcg ctggctatcc cgaacctcga tgacggcaac
      841 cagatcatgt ggcaactggt gcgcaaagac attgtttctt cgccggacct ggcgccaaag
      901 cacggctgga tcgatgcttt ccaaggtcca gggctggggt ttgagctgaa tgaagacgtg
      961 atcgccgagg gagagcgggg cttcaaggca tcgaaggcgg catgacccgg ccccctgccc
     1021 gcatacccgc cggcgacgcc gaaaaaacgg cgatcgcggc gctgatgcga agtggggcgt
     1081 taccggagaa tgcattgccg gtagcccgag ccatggtgcg ggccgagatc gaaggcaatg
     1141 ccgtctgcgg cctctattac ctgccgatct tctgcgcgca tcttggctgc ggaaaggtcg
     1201 acggcaaggc gaaaccgcag gtcgtgcccg accaggcggc aatcaaagtg gatgcgcgcc
     1261 atggtttcgc gcatccggcc atcgaagctg ggcttccgca tctgatcgac gctgcgagga
     1321 cattcggcat cgcggcgctg tcgatccgca attcctataa ttgcctggca ttatcgcatc
     1381 acgttcggcc gctggccaaa gaggggttga tcggcctgag catggcgaac gcgccggcat
     1441 ccgtggcgcc gcccggcgcg acccgcgcca ttttcgggac caacccgatt gccttcgccg
     1501 ccccgatggc ggacggcgac atgatcgtcg tcgaccaaag catgagcgcc gtcaccaaga
     1561 ccgagatggc gatgcgccgg gaccggggcc agccgatacc caccggctgg gcgcaagaca
     1621 ggtccggcgc gccgaccacc gacgcaggcg aaggcctgcg cggatcgctg ctgcccagcg
     1681 gcgggcaaaa gggcgccaat ctggcgctga tgatcgaaat attggcgacg gcgctaaccg
     1741 gggcggagct gagttcgcag gccagcatgt tcggcgacaa tcaaggcggg ccgccgggcg
     1801 tcggccaatt catgctggcg atcgatcccg cccgcttcag cgccggcttt gcggaaagcc
     1861 ttggcgccct tgcggaaagt ttccaggccg ccggagtgcg attgccgggc agcggagcga
     1921 cagcgggcaa acaggatcgg gattcaatcc tgatcgacgt cgatgccgat ctgtggcaac
     1981 gctgcctcga cctcgcgcag ggccggtagc actggcgaag cccggctggc gaaacttaat
     2041 agtaaaaggc ggcccggact gtcgtgtcga gaa
//
LOCUS       DASYCS010000415         8895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4328071,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000415 DASYCS010000000
VERSION     DASYCS010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 8895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8895
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4328071"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1622)
                     /gene="ilvD"
                     /locus_tag="VGM46_12765"
     CDS             complement(<1..1622)
                     /gene="ilvD"
                     /locus_tag="VGM46_12765"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003531150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEY4193511.1"
                     /translation="MPAYRSRTTTHGRNMAGARGLWRATGMKDGDFGKPIIAVVNSFT
                     QFVPGHVHLKDLGQLVAREIEKAGGVAKEFNTIAVDDGIAMGHDGMLYSLPSREIIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLNAAMRLNIPVVFVSGGPMEAGKVVLGGKTQ
                     ALDLVDAMVAAADDHVSDEDVKIIERSACPTCGSCSGMFTANSMNCLTEALGLSLPGN
                     GSTLATHADRKRLFVEAGHLIVDLAQRYYEQDDETALPRNIATKGAFENAMTLDIAMG
                     GSTNTVLHILAAAHEGEVDFTLDDIDRLSRRVPCLCKVAPAKADVHMEDVHRAGGIMA
                     ILGQLDRAGLLNRDLPTVHTATIGQAIEHWDISSTNSQTVRDFYLAAPGGVPTQEAFS
                     QARRWDDLDLDREKGVIRSAEHPFSKDGGLAVLKGNVAVDGCIVKTAGVDESILKFTG
                     PARVFESQDSSVLAILNGKIKPGDVVVIRYEGPRGGPGMQEMLYPTSYLKSKGLGKVC
                     ALLTDGRFSGGTSGLSIGHVSPEAEEGGLIGLV"
     gene            1877..2350
                     /locus_tag="VGM46_12770"
     CDS             1877..2350
                     /locus_tag="VGM46_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY4193512.1"
                     /translation="MQKITPCLWFKNEAEEAVDFYVSIFPDARKGTVLRSGESGPGPV
                     GSVIMVNFELAGQSFQALNGNSDDPFNNSISLSVDCKDQAEVDRYWEKLIDGRGKEIA
                     CSWLKDRYGVSWQIVPEILPRLLNDPDKAKAGRVMQAMMKMVKIDVAAIEDAAKG"
     gene            complement(2424..5507)
                     /locus_tag="VGM46_12775"
     CDS             complement(2424..5507)
                     /locus_tag="VGM46_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY4193513.1"
                     /translation="MSISDLFIRRPVLSTVLACMILLLGFQGIFNLSIRQYPKVDETA
                     ITITTAYPGASADLIQGFISAPIARAVSSTENVDYVTSQSTPSASVVTVQMKLGSNPD
                     VALTEVLSKVQGVRGTLPEESKDPVIVKGTGQQFAMMYLSMQNPNMTPEQLTEYIERV
                     VRPRMTTVEGVADVQIFGAADYSMRVWIDPVKLAARGVTATDVLTAINNSNFLSAPGK
                     TENEYVASAINVRSTLQTPEAFAQLPLRSQDGNVVRLRDVARVELAAESTDTRVNFNG
                     KPGTFLAIFPTPAANPLSTAEAIHKIVPSIQETLPKGMTIEIVYDSTEQISASIDEVF
                     KTIGEAVAIVVVVILLFLGSFRSVAMPVVTIPLALVGVCFILFAVGYSINLLSLLAMV
                     LAIGLVVDDAIVVVENIHRHMEEDNMPPMQAAFNGMREISSAIIAMTMTLAAVFAPLA
                     FTGGLTGALFREFAVTLAGAVVLSGIVAITITPMMSARILKSGSHSRFQKIVDGTFAR
                     VEHVYERLVTGSLKYRPVTLMIVIALVALTGFMFTKTSSELAPEEDQGFLLSLVTGPR
                     YATSDYTETYVNQILGLVKDVPETRALFAATAFGGATNTAFVGFAFKDWADRARSQKE
                     LQADIQGRLGKVAGVQAFVFAPPTLPGSGGGLPIAMVVRSTGEASQVYEAAEAIKNKA
                     QASGRFIVVQNSMSFDAPQVTVTIDRDRAAALNIPISDIGRTLTLLVGGAEVAQFDRD
                     SNSYDIIPQVPQEFRNNPEKLGDYFVRSVTGDMVPLSAVVKISTNASPAAIEQFNQLN
                     SATISALPVPGVTTGDGLKTLEDIANQSLPDTFFVDYSGQSRQEKEQGNTILIAFGAA
                     ILVIYLVLAAQFESFRDPLIIMMSVPLSIFGAIVPLNLGLGTLNIYTQVGLITLIGLI
                     TKHGILLVEFANQQREVHGMRRRDAIVASAKVRLRPILMTTAAMALGVVPLITSSGAG
                     AAARYSMGLVIFTGILIGTMFTLFVVPMFYTLIASKDLPQQAEKPDPKLMPVMVE"
     gene            complement(5504..6733)
                     /locus_tag="VGM46_12780"
     CDS             complement(5504..6733)
                     /locus_tag="VGM46_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY4193514.1"
                     /translation="MIKRFIIAFILLVLICGGIVGFNIFRDNAIKQYFATMKPPAATV
                     STTVVKPISWTPGIEAIGTVSAVRGVDLTVETAGIVKEILFQANHKVAAGAVLVQLDD
                     AVERADLDAQKAQAALDQTALTRAGELQKRGVGSDVTLDTARATASASASQVSKLQAV
                     LDQKQLSAPFGGTPGIPKIDLGQYLTPGTAVVTLQDLDTMRVDFTIPEQQLPLVKIGQ
                     TVKLGVGDDKMPYAGTIRGINPKIDPASRLVTIRAEVDNSEGKLSPGQFVQVRVELPE
                     ENNVLALPQTALTTSLYGDFIFVVHPAKPAEANAGAAATEKPAADAAKPAADAKPATD
                     PAKPAADEQKPVLVLSQVFVTPGRRNGDKVEILKGIADGDEVVTAGQNRLFNGMSVNV
                     DNTVDPTKPANPQAAAQ"
     gene            6884..7444
                     /locus_tag="VGM46_12785"
     CDS             6884..7444
                     /locus_tag="VGM46_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEY4193515.1"
                     /translation="MKLPRSNSREKILDAAADVAAEAGPGSLSLEAVALRAGVSKGGL
                     LYNFPTKAKLMQGLVERYLSEFEQALENARANCTNGENLLSIYIRLAAMECEGTKPSA
                     SWIFSAIAEDPDFLTPIKAFRQALFQRLKAETPNTKALLVCYLAIEGMRSMNLFDSHI
                     LTDAERETLVESLLAVAEPQREPALH"
     gene            complement(7452..8285)
                     /locus_tag="VGM46_12790"
     CDS             complement(7452..8285)
                     /locus_tag="VGM46_12790"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006200741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEY4193516.1"
                     /translation="MLGMVCGCTTTPPEQVLTVPSSAGKYAAIVVDARTGKQLFEMNS
                     TAPRYPASLTKMMTLYLLFEALDSGRVNKLTLIPVSQHAASQPPTKMRLKAGETIDVD
                     SAIHAITVRSANDVAVAVGEFLGGSEEAFAAMMTAKARQIGMRNTVFRNASGLPDDAQ
                     HTTARDMAVLGMALRQRFPQYYGYFSTADFMFRSRMIRGHNDMLGRVPGVDGIKTGYI
                     RASGFNIVTSYDAGGRRIIVVVMGADSARARNAHAEALLRQALLPDAGDAKLVFADPQ
                     P"
     gene            8480..>8895
                     /locus_tag="VGM46_12795"
     CDS             8480..>8895
                     /locus_tag="VGM46_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006200743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193517.1"
                     /translation="MDTVTIRANGISVSLDLGVGHLADMAVEAGGRILRPLHRAPWID
                     EPKESLPENLPEGVVRLSGDFLCAPFSRSDVEEAPLHGWTANSRWETLESAATDDGWR
                     ARFRLHRKVMGASVEKVLTLRDGHPFLYQEHLFSGGA"
BASE COUNT         1655 a         2665 c         2919 g         1656 t
ORIGIN      
        1 accagcccga tcagcccgcc ctcctcggct tccggcgaga catggccgat ggacaggccg
       61 gaggtgccgc cggaaaaacg gccatcggtc agcagcgcgc agactttgcc caggcctttc
      121 gacttcagat agctggtcgg atagagcatt tcctgcatgc cggggccgcc gcgcgggcct
      181 tcatagcgga tcaccaccac gtcgcccggt ttgatcttac cgttcaagat ggcgaggacg
      241 ctggaatcct ggctttcgaa cacgcgcgcc gggccggtga acttcaggat cgattcatcg
      301 acgcccgccg tcttcacgat gcagccatcg acggcgacgt tgcccttcag cacggccagc
      361 ccgccatcct tggagaaggg gtgctcggcg gaacggatga cacccttttc gcggtcgagg
      421 tcgaggtcgt cccaacgccg ggcctggctg aaagcttcct gcgtcggcac gccgccgggc
      481 gcggccagat agaaatcacg cacggtctgg ctgttggtcg aggaaatgtc ccaatgctcg
      541 atggcctggc cgatggtggc cgtatggacg gtcggcaggt cgcggttgag aagcccggcg
      601 cggtcgagct ggccgagaat ggccatgatg ccgccggccc ggtggacgtc ttccatgtgc
      661 acgtcagcct tggccggcgc caccttgcac aggcaaggca cgcggcgcga cagccggtcg
      721 atgtcgtcga gcgtgaaatc cacctcgcct tcatgggcgg cggcgaggat atgcagcacc
      781 gtgttggtcg agccgcccat ggcgatatcg agcgtcatgg cgttctcgaa cgcgcccttg
      841 gtggcgatgt tgcgcggcag cgccgtctcg tcgtcctgct cgtaataacg ctgggccagg
      901 tcgacgatca ggtggcccgc ctcgacgaac agacgcttgc ggtcggcatg cgtcgctagc
      961 gtcgagccgt tgccgggtag cgacaggccg agcgcctcgg tcaggcagtt catggaattg
     1021 gcggtgaaca tgcccgaaca cgagccgcag gtcgggcaag cggaacgctc gatgatcttg
     1081 acgtcttcgt cggagacatg gtcgtcggcg gccgccacca tggcgtcgac cagatcgagc
     1141 gcctgcgtct tgccgcccag cacgaccttg ccggcttcca tcgggccgcc ggaaacgaag
     1201 acgacgggaa tgttcaggcg catggcggcg ttgagcatgc cgggcgtgat cttgtcgcaa
     1261 ttggaaatgc agaccatggc gtcggcgcaa tgcgcgttga ccatgtattc gaccgagtcg
     1321 gcgatgatct cgcggctggg cagcgaatag agcatgccgt catggcccat ggcgatgccg
     1381 tcatcgacgg cgatggtgtt gaattccttg gcgacgccgc ccgccttctc gatctcgcgc
     1441 gcgacaagct ggccgaggtc cttcaggtgg acatggcctg gcacgaactg ggtgaaggaa
     1501 ttgaccaccg cgatgatcgg cttgccgaaa tcgccgtcct tcatgccggt agcgcgccag
     1561 aggccgcgtg cgccggccat gttgcggcca tgggtggtgg tgcgagagcg ataggcaggc
     1621 atgaaactgt ccttgatgca tatgccgcgc cgtcaagaca cgggaaacga aaatggaacc
     1681 gcaggcgtta tatacgccct tgcatggtct tgccatgttt ttgcgatgcg ccagttttag
     1741 cccgttacaa cgccgcgcaa tttttttcag gccggtgtcg gacccggccg acgccacccg
     1801 tccttggaac aatggcgcat gccgttttac cagtccgatc aatcatgacc atacaagtca
     1861 cgccgaggag aaagtcatgc agaaaatcac cccatgcctg tggttcaaga acgaagccga
     1921 agaagccgtc gatttctatg tttcgatttt tccggacgcc cggaaaggaa cggttctgcg
     1981 aagcggagaa agcggccccg gcccggtggg cagcgtcatc atggtcaatt tcgagttggc
     2041 cgggcagtcg tttcaagccc tcaacggcaa ttccgacgac ccgttcaaca attcaatctc
     2101 gctctccgtc gactgcaagg accaggccga ggtcgaccgc tattgggaga agctgattga
     2161 cgggcgcggc aaggagatcg cgtgcagctg gctgaaagac agatatggcg tctcgtggca
     2221 gatcgtgccg gaaattctgc cgcgcctgct caacgacccc gacaaggcga aggccggccg
     2281 cgtcatgcag gcgatgatga agatggtcaa gatcgacgtc gccgcgatcg aggacgcggc
     2341 gaaaggctga atacggcttt cgcccaagga aaaaggcggt ggatcgctcc accgcctttt
     2401 ttgttttggt attcgcagcc ggtctattcg accatgaccg gcatcagctt gggatcgggc
     2461 ttttccgcct gttgcggcaa atccttgctg gcgatgagcg tatagaacat cggcaccacg
     2521 aacagcgtga acatggttcc gatgagaatg ccggtgaaga tcaccaggcc catcgaatag
     2581 cgcgccgcgg cacccgcgcc gctggaggtg atcagcggca ccacgccgag cgccatggcg
     2641 gcggtcgtca tcaggatcgg gcgcaggcgc accttggccg aggcgacgat ggcgtcgcgc
     2701 cggcgcatgc catgcacttc acgctgctgg ttggcgaact ccaccaaaag gataccgtgc
     2761 ttggttatca ggcctatcag cgtgatcagg ccgacctgcg tgtagatgtt cagcgtgccc
     2821 agccccagat tgagcggcac gatggcgcca aagatcgaca gcggcaccga catcatgatg
     2881 atcagcggat cgcggaagct ttcgaactgc gccgccagca ccagatagat gacgaggatg
     2941 gctgcgccaa acgcgatcag gatggtattg ccctgctcct tttcctgccg cgactggccg
     3001 gaataatcga cgaagaaggt gtccggcagg ctctggttgg cgatgtcctc cagcgtcttc
     3061 agcccgtcgc cggtggtgac gcccggcacg ggcagcgccg agatggtcgc cgaattcagc
     3121 tggttgaact gctcgatggc cgccggcgaa gcgttggtcg atatcttcac caccgccgaa
     3181 agcggcacca tgtcgccggt tacgctgcgg acgaaatagt cgcctagctt ctctggattg
     3241 ttgcggaact cctgcggcac ctgcgggatg atgtcgtagc tgttggagtc gcggtcgaac
     3301 tgcgccactt ccgcgccgcc caccagcagc gtcaacgtgc ggccaatgtc gctgatcggg
     3361 atgttgagcg ccgccgcgcg gtcgcggtcg atggtcaccg tcacctgcgg cgcatcgaag
     3421 gacatcgaat tctgcacgac gatgaagcgg cctgaagcct gcgccttgtt tttgatcgct
     3481 tcggcggctt catacacctg gctagcctcg cccgtggagc gcaccaccat ggcgatcggc
     3541 aagccaccgc cggaccccgg caaggtggga ggagcgaaca cgaaggcctg cacgccggcc
     3601 accttgccca atcgaccctg aatgtcggcc tgaagttcct tttgcgagcg ggcgcggtcg
     3661 gcccaatcct tgaaggcgaa gccgacgaag gcagtgttgg tggcgccgcc aaaggcggtg
     3721 gctgcaaaca gcgcgcgcgt ttccggcacg tccttcacca ggcccaatat ctggttgacg
     3781 taggtttccg tgtagtcgga cgtggcgtag cgcggaccgg tcaccagcga cagcaggaag
     3841 ccctggtctt cttccggggc cagctcgctg gaggttttgg tgaacatgaa gccggtcagc
     3901 gccaccagcg caatgacgat catcaaggta accgggcggt atttcagcga tccggtgacc
     3961 agtctttcat agacatgttc gacgcgcgcg aacgtgccgt cgacgatctt ctggaagcgg
     4021 ctgtgcgagc cggacttcag gatacgcgcc gacatcatcg gcgtgatggt gatggcgacg
     4081 atgcccgaca gcaccacggc accggcgagc gtgaccgcga actcgcgaaa cagcgcgccc
     4141 gtcaagccgc cggtgaaggc gagcggcgcg aacacggcgg ccagcgtcat cgtcatcgcg
     4201 atgatggccg aggagatttc gcgcatgccg ttaaaggccg cctgcatcgg cggcatgttg
     4261 tcttcttcca tgtggcggtg aatgttttcc accaccacga tggcgtcgtc gacgacaagg
     4321 ccgatggcga gaaccatggc gagcagcgac agaagattga tcgaatagcc gacagcaaac
     4381 aggatgaagc agacgccgac caaggcgagc gggatagtga cgaccggcat ggcgaccgat
     4441 cggaacgacc ccaggaacaa aagaatgacc accaccacga tggcgaccgc ctcgccgatg
     4501 gtcttgaaca cctcgtcgat ggacgccgat atctgctcgg tggaatcgta gacgatctcg
     4561 atcgtcatgc ccttgggcag cgtttcctgg atcgacggca cgatcttgtg gatcgcctcc
     4621 gccgtgctca gcgggttggc cgcgggcgtc gggaagatgg cgaggaaggt gcccggcttg
     4681 ccgttgaaat tgaccctggt gtcggtgctt tcagccgcca gttccacgcg ggcaacgtcg
     4741 cgcaggcgca ccacattgcc gtcctgcgag cgcagcggca gctgggcgaa agcctctggc
     4801 gtctgcaatg tcgagcgaac gttgatcgcg gaggcgacat attcgttctc ggtcttgccg
     4861 ggcgcggaaa ggaagttgga attgttgatg gccgtcagca cgtcggtggc ggtgacgccg
     4921 cgcgccgcca gcttgaccgg atcgatccag acgcgcatcg aatagtcggc ggcgccgaat
     4981 atctgcacat cggccacgcc ttcgaccgtc gtcatgcgcg gccgcacgac gcgctcgata
     5041 tattcggtca gctgttccgg cgtcatgttc ggattttgca tcgacagata catcatcgcg
     5101 aattgctggc cggtgccctt gacgataacc gggtccttgg attcttccgg cagcgtgcca
     5161 cgcacgccct gaaccttgga cagcacttcc gtcagcgcga catccgggtt ggagccaagc
     5221 ttcatctgca cggtcacgac gctcgccgag ggcgtgctct gcgaggtgac gtaatcgaca
     5281 ttctcggtcg aggagacggc gcgcgcgatc ggcgccgaaa tgaagccctg gatcaggtcg
     5341 gcgctggcgc ccggataggc ggtggtgatg gtgatcgccg tctcgtcgac ctttggatac
     5401 tggcggatcg acaggttgaa gatgccctgg aagcccagga gcaggatcat gcaggcaagc
     5461 acggtcgaaa ggaccgggcg gcggatgaag agatcggaaa tactcattgc gcggcggcct
     5521 gcgggttggc gggcttggtc ggatcgaccg tgttgtcgac attgaccgac atgccgttga
     5581 acagccggtt ctggccggcg gtgacgacct cgtcgccgtc ggcgatgccc ttcaatattt
     5641 ccaccttgtc gccgttgcgg cggccgggcg tgacgaacac ctgggaaagg accaggacgg
     5701 gcttctgctc gtcggcggca ggcttggccg gatcagtggc gggcttggcg tcggcggcag
     5761 gcttggccgc gtcggctgcg ggcttttctg tcgccgccgc gccggcattc gcctcggcag
     5821 gcttggccgg gtgaaccacg aagatgaagt cgccgtaaag gctcgtggtc agcgcggtct
     5881 gcggcagggc cagaacgttg ttttcttcgg gcagttcgac acgcacctgc acgaactggc
     5941 cggggctgag cttgccttcg ctgttgtcca cttcggcccg gatcgtcacc agccggcttg
     6001 ccggatcgat cttcgggttg atgccgcgaa tggtgccggc gtaaggcatt ttgtcgtcgc
     6061 ccacgccaag cttcaccgtc tgcccgatct tgacgagcgg cagctgctgt tcgggaatgg
     6121 tgaaatcgac ccgcatcgtg tcgagatcct gtagcgtcac gacggcggtg ccgggggtca
     6181 ggtactggcc gagatcgatc ttgggaatgc cgggcgttcc gccaaacggc gcgctcaact
     6241 gcttctggtc gagaacggct tgcagcttgc tgacctgcga ggcggaggcc gaggccgtcg
     6301 cccgcgccgt atccagcgtt acatccgagc cgacgccgcg cttttgcagt tcgccggcgc
     6361 gggtcagcgc cgtctggtcg agcgcggcct gggctttttg cgcatcgaga tcggcgcgct
     6421 cgaccgcatc gtccagctgg acgaggaccg cgcctgccgc caccttatgg ttggcctgga
     6481 acaggatttc cttgacgatg ccggccgtct cgaccgtcag gtcgacgccg cgcacggcgc
     6541 tgacggtgcc gatggcctcg atgccgggcg tccaggaaat cggcttcacc accgtcgtcg
     6601 agacggttgc cgccggcggt ttcatcgtgg cgaaatactg cttgatcgca ttgtcgcgga
     6661 aaatattgaa gccgacaatg ccgccgcaaa taagcacaag caggatgaag gcgatgatga
     6721 agcgcttgat caagaaatgt cccctcggtc gtaccgggta attgatgacg gaaatgcagg
     6781 cgcacgccgt cgcacgatgc cggcctaaaa tccttctccg cttactgtac cgtctggacg
     6841 gtcttgtcaa ttgggtttag gtacgcagga accggagaac gcaatgaagc tgccaagatc
     6901 gaattcgcgt gagaaaatcc tcgacgccgc cgcggacgtt gcggccgaag ctgggccggg
     6961 cagtctttcg cttgaggcgg tggcgctgcg cgccggcgtt tccaagggcg ggcttctgta
     7021 taatttcccc accaaggcaa agctcatgca gggactggtc gagcgctacc tcagcgaatt
     7081 cgagcaggca ctggaaaatg cccgcgcaaa ctgcaccaat ggcgaaaacc tgctttccat
     7141 ctatatcagg ctggcggcca tggagtgcga gggaaccaag ccctccgcct cgtggatatt
     7201 ctcggccatc gccgaggacc ccgattttct gacgccgatc aaggcattcc ggcaagcgct
     7261 gtttcagcgt ctcaaggcgg aaacaccgaa tacgaaggcg cttctggtct gctatctcgc
     7321 catcgagggc atgcgcagca tgaacctgtt cgattcccac atcctgacgg atgcggagcg
     7381 cgaaacgctg gtcgaatcgc tgctggcggt cgccgaacca cagcgcgaac cggccttgca
     7441 ttaaaccggg ttcagggctg cgggtcggcg aagaccagct ttgcatcgcc cgcatcaggc
     7501 agcagggcct gccgcaacag cgcttcggcg tgcgcgttgc gggcgcgggc actgtccgcg
     7561 cccatcacca cgacgatgat gcgacggcct ccggcatcgt aggaagtgac gatgttgaaa
     7621 ccggaagcgc ggatatagcc ggtcttgata ccgtcgacgc ccggcacccg gcccagcatg
     7681 tcgttgtggc cgcggatcat gcggctgcgg aacatgaaat cggccgtgga gaaatagccg
     7741 taatattgcg ggaaccgctg gcgcagcgcc atgcccagca ccgccatgtc gcgcgcggta
     7801 gtgtgctgcg catcgtcagg caggccggat gcgttgcgga aaacggtgtt gcgcatgccg
     7861 atctggcgcg ccttggcggt catcatggcg gcaaacgctt cttcgctgcc gccaaggaac
     7921 tcgcccaccg caacggccac gtcgttggcc gagcgcacgg tgatggcatg gatggcgcta
     7981 tcgacatcga tggtttcgcc ggctttgagc cgcatcttgg tcggcggctg cgaggcggcg
     8041 tgttgggaga cgggaatgag cgtcagcttg ttgacgcggc ctgaatccag cgcctcgaac
     8101 aacaggtaca gcgtcatcat cttggtcagg gaagcgggat agcgcggcgc ggtcgaattc
     8161 atttcgaaca gttgcttgcc ggtcctggca tcgacgacga tggctgcata tttgccggcg
     8221 gaggacggca ccgtcaaaac ctgttcgggc ggtgtggtcg tgcagccgca aaccatgccg
     8281 agcatggcga tggcgacgag gcatttctgg atgaaggaaa tcgggcgcaa gatcgttctg
     8341 ttcggtgggc aagccgttgc ggagacgaga cgtctctatc gtaagcccgc gcccgcgtcc
     8401 atttggttaa tgcaaatggt gcttgcgccg gggcctgccg aaaggcttcc ctttagccaa
     8461 cgcgaatcgg tggtggacaa tggatactgt cacgatcagg gcaaacggca tttcggtctc
     8521 gctggacctt ggcgtcggcc atcttgccga catggccgtc gaggccggcg gccggatttt
     8581 gaggccgctg caccgcgcgc cctggataga cgagccgaag gaaagcttgc cggaaaatct
     8641 gcccgagggc gtcgtgcggc tttccggcga tttcctgtgc gcgccatttt cgcgcagcga
     8701 cgtggaggaa gcgccgctgc atggctggac ggccaatagc cgctgggaga cgctggaaag
     8761 cgcggcaacc gacgatggct ggcgcgcgcg ctttcgcctg caccgcaagg tgatgggggc
     8821 gagcgtcgaa aaagtcctga cgctgcgcga cggccatccg ttcctttatc aggaacatct
     8881 gttttcgggc ggcgc
//
LOCUS       DASYCS010000416         2286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2008916,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000416 DASYCS010000000
VERSION     DASYCS010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2008916"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..781)
                     /locus_tag="VGM46_12800"
     CDS             complement(188..781)
                     /locus_tag="VGM46_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010915530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutD family protein"
                     /protein_id="HEY4193518.1"
                     /translation="MRILRAGDYREMPWKNGGGTTTEIAVSPANAGIDAFDWRISMAR
                     VEGGGPFSLFPGIDRTLSILEGEGIVLDIDGREPVRLTRSSEPLSFPADAATSATLIG
                     GTVIDLNVMTRRGRASHYVQRIAVSGTVEIEAGADADAVAVFCAAGHVSVQGVTAAGL
                     GPRDTLFFGSQSRPLRLESHGQASLFVIRITIGDHAV"
     gene            complement(787..>2286)
                     /gene="hutU"
                     /locus_tag="VGM46_12805"
     CDS             complement(787..>2286)
                     /gene="hutU"
                     /locus_tag="VGM46_12805"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018427240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEY4193519.1"
                     /translation="ARDWESFDRIVSALRKLEADETLLVQSGKPVGVFRTHKDAPRVL
                     IANSNLVPHWATWEHFNELDKKGLMMYGQMTAGSWIYIGSQGIVQGTYETFVELGRQH
                     YNGELSGRWILTAGLGGMGGAQPLAATMAGASCLAVECQASRIEMRLKTGYVDVQAKD
                     LDDALAIIDKAGKEKKAISVALLGNAADIFPELVKRGVKPDAVTDQTSAHDPINGYLP
                     QGWTVAQWEEKRERDPKSVDKAARASMALHVQAMLDFQKMGVPTFDYGNNIRQMAFEE
                     GVKDAFNFPGFVPAYIRPLFCRGIGPFRWAALSGDPEDIYKTDAKVKELTPGNYHLHN
                     WLDMARERIHFQGLPARICWVGLGDRHRLGLAFNEMVASGELKAPVVIGRDHLDSGSV
                     ASPNRETEAMKDGTDAVSDWPLLNALLNTASGATWVSLHHGGGVGMGFSQHAGMVIVA
                     DGTEDAARRLERVLWNDPATGVMRHADAGYDIAIDCAKEHGLNLPGILG"
BASE COUNT          409 a          728 c          738 g          411 t
ORIGIN      
        1 cgaacaaaag tcgtggagtg acgtcgccga ccgacaataa cagccgtggc tgccgcgaca
       61 accggcagac acaggctttt gttccactcg gttaggagcg agaggaaagg attcgggctt
      121 tttcgtgttt cagggcacgc gtggggccag aatcgggagg cgatgcggta acgctttgag
      181 accgccgcta aacggcgtga tcgccgatcg ttatcctgat cacgaacaaa cttgcctgcc
      241 cgtgcgattc gaggcgaagc gggcgggatt gcgaaccgaa gaacagcgtg tcgcgtgggc
      301 caagcccggc tgcggtcacg ccttgcacgg agacgtggcc tgccgcgcaa aacaccgcaa
      361 cggcgtccgc atcggcgccg gcctcgattt cgaccgtgcc ggaaacagcg atccgctgga
      421 cataatggct cgccctgccg cgcctcgtca tgacattcag gtcgatgacc gtgccgccaa
      481 tcaaggtggc gctggtcgca gcgtcggccg ggaaggacag cggttcggag ctacgcgtca
      541 ggcgaacagg ctcgcggcca tctatgtcga gcacgatgcc ttcgccttcc aggatggaaa
      601 gcgtgcggtc gatgccggga aacagggaga acggcccgcc accttccacc cgcgccatcg
      661 agattcgcca gtcgaaggcg tcaatgccgg cattggccgg cgaaacggcg atctcggtcg
      721 tcgtcccgcc gccgttcttc cacggcattt cccggtagtc gcccgcgcga aggatgcgca
      781 tcgcgctcag cccaggatgc ccggcaggtt caagccatgc tctttcgcgc agtcgatggc
      841 gatgtcgtag ccggcatcgg catggcgcat gacgccggtt gccggatcgt tccacagcac
      901 gcgctccagc cggcgcgccg catcctcggt gccatcggcg acgatgacca tgccggcatg
      961 ctgcgaaaag cccatgccga cgccgccgcc atggtgcagc gaaacccagg tcgcgcccga
     1021 tgcggtatta agcagcgcgt tgagcagcgg ccagtctgac acggcgtcgg tgccgtcctt
     1081 catcgcttcc gtctcgcggt tgggcgaagc gaccgagccg gaatcgaggt ggtcgcggcc
     1141 gatgaccacc ggcgctttca gttcgccgct ggccaccatt tcgttgaagg cgaggcccag
     1201 ccggtgacgg tcgccaagac cgacccagca aatgcgcgca ggcaggccct ggaaatggat
     1261 gcgctcgcgc gccatgtcca gccagttgtg cagatggtag ttgccggggg tcagttcctt
     1321 caccttggcg tcggtcttgt agatatcttc cgggtcaccc gacagggccg cccagcggaa
     1381 cgggccgatg ccgcggcaaa acagcgggcg gatataggcc ggcacgaagc ccggaaaatt
     1441 gaaagcgtcc ttgacgcctt cttcgaacgc catctggcgg atgttattgc cgtagtcgaa
     1501 ggtcggcacg cccatcttct ggaaatcgag catggcctgc acatggagtg ccatggaggc
     1561 gcgggcggcc ttgtctaccg atttcgggtc gcgctcgcgc ttttcttccc actgagccac
     1621 ggtccagccc tgcggcagat agccgttgat cggatcgtgc gccgaggtct ggtcggtgac
     1681 ggcatccggc ttgacgccgc gcttgaccag ttccgggaag atatcggcgg cgttgccgag
     1741 cagggccacg gaaatcgcct tcttttcctt gccggccttg tcgatgatcg ccagcgcgtc
     1801 atcgagatcc ttggcctgaa cgtcgacata gccggtcttc agccgcatct cgatgcggct
     1861 ggcctggcat tcgacggcca ggcacgaggc gccggccatg gtggcggcca gcggctgtgc
     1921 accgcccatg ccgccgaggc cggcggtcag tatccagcgg cccgacagct cgccattata
     1981 atgctggcgg ccaagctcga cgaaggtttc gtaagtgccc tgaacgatgc cctgcgagcc
     2041 gatgtaaatc cacgagccgg ccgtcatctg gccgtacatc atcaggccct tcttatcgag
     2101 ctcgttgaaa tgttcccatg tcgcccaatg cggaacgagg ttggaattgg cgatcagaac
     2161 gcgcggcgca tccttgtggg tgcgaaacac gccgaccggc ttgccggatt gcaccagcag
     2221 cgtttcgtcg gcttccagct tgcgcagcgc gctgacgatg cggtcgaaac tttcccagtc
     2281 gcgcgc
//
LOCUS       DASYCS010000417         3669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4903667,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000417 DASYCS010000000
VERSION     DASYCS010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4903667"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..707
                     /gene="pth"
                     /locus_tag="VGM46_12810"
     CDS             99..707
                     /gene="pth"
                     /locus_tag="VGM46_12810"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEY4193520.1"
                     /translation="MDTPLLIAGLGNPGSQYAHNRHNAGFIVLDELHAHYRFGPWKAK
                     FDGLLSEGSLGGRKTYLLKPQTFMNLSGDCIGPSLRFYKLPLEALVVVHDEIDLAAGK
                     VKVKTGGGDAGQNGLRSITATLGPDYRRVRLGIGHPGEKSRVSGHVLQNFSKEDIDWL
                     RPLVGAMVEAAPLLARDDDAGFMSKVALLTAPVKPPKKKDTE"
     gene            707..1804
                     /gene="ychF"
                     /locus_tag="VGM46_12815"
     CDS             707..1804
                     /gene="ychF"
                     /locus_tag="VGM46_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEY4193521.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTQTAAAQAANYPFCTIEPNVGD
                     VAVPDPRLETLAGIAGSQEIIPTRITFVDIAGLVRGASKGEGLGNQFLANIREVDAVA
                     HVLRCFIDDDITHVEGKIDPVADAETVETELMLADLESLEKRLKPLEKKANSGEKEAK
                     EQLALMMKAVTLLREGKPARRADLAPEERAPYSQLGLMTAKPVLYVCNVEEGSAATGN
                     AISAKVEAMAKAEGARSVVISAKIEEEIAQMPMEDRKDFLDSLGLEEPGLNRLIRTGY
                     DLLNLLTFFTVGPKETRAWTVTRGSKGPQAAGVIHTDFEQGYIRAETIAYPDYVAGKG
                     EAGAREAGKFRLEGKEYVVADGDVMHFRFAN"
     gene            1871..2755
                     /locus_tag="VGM46_12820"
     CDS             1871..2755
                     /locus_tag="VGM46_12820"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEY4193522.1"
                     /translation="MLPISDELQRQRLAVLATIETDPYRSATTIGIEEVLDAHYLIAD
                     LFAARGQSFVAVGPARIELLHSALHRQFVVLGGRPKWSTPSEIAATVLFGIAKNHAFY
                     DANKRTALVSVLWLLFEKYNLSPTASQKALEDFVVDVADDQLAEKYARCKASKQSGDP
                     DAEVRFIAYWIKENTRKMQNDYRTITFRELRKIMRRYNFEFENPKDNQIDIVNVEQHS
                     GLFGIGAREVRKRVGRIGYHSETTQVAKSDIKKLRERCGLTFKSHMVDSLEFYDGVNP
                     MNFLLAEYQDILVSLADR"
     gene            complement(2756..>3669)
                     /locus_tag="VGM46_12825"
     CDS             complement(2756..>3669)
                     /locus_tag="VGM46_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY4193523.1"
                     /translation="AIAADIAENAVAVITDAEAMGEYRSISEFDMFEVEYWSLEQAKL
                     GKSNEKALSRQVAVITGGASGIGAATAKAMAKQGAEVAILDRDLDAAKVAAKKIGGKT
                     LAVQCDVTDPMSVRKAFDAVVSAFGGVDIVVSNAGAAWQGTIGHVDDETLRKSFELNF
                     WAHQAVAQHATRIMLAQGTFGVLLFNTSKQAVNPGKDFGPYGLPKAATLFLVKQYALD
                     HGKDGIRANAVNADRIRSGLLTDDMVAKRSAARGVSESDYMAGNLLKREVTAEDVADA
                     FVYLATASKTTAAVVTVDGGNIEASLR"
BASE COUNT          823 a         1035 c         1094 g          717 t
ORIGIN      
        1 gtgccgcgcc tgccgccggc gccaaggccg cgccggccgc cgccgccaag cctgcggcga
       61 agaagtaaga taccgcgcca gggggaggcg tctccgtcat ggatacgcct ctcctcatcg
      121 ccggtctggg caatcccgga tcccaatatg cgcacaaccg gcacaatgcc ggcttcattg
      181 tgctggacga actgcacgcg cattaccgct tcggcccatg gaaggccaaa ttcgacggtc
      241 tgctgtcgga aggctcgctt ggtgggcgca aaacctatct gctgaagccg cagaccttca
      301 tgaacctgtc gggcgactgc atcgggccga gcctgcgctt ctacaagctg ccgctggaag
      361 cgctggtcgt ggtgcatgac gagatcgatc ttgcggcagg caaagtgaaa gtcaaaaccg
      421 gcggcggcga tgcggggcag aacgggttgc gttccataac cgccaccttg ggtccggact
      481 atcgccgcgt gcggttgggc atcggccatc ccggcgagaa gtcgcgagtg tcgggccatg
      541 tgctgcagaa tttctcgaaa gaagatatcg actggctgcg accgctggtc ggcgccatgg
      601 tcgaagcggc gccgctgctg gcacgcgacg acgatgccgg attcatgagc aaggtggcgc
      661 tgctgaccgc gcctgttaaa ccaccgaaga agaaagatac cgagtaatgg gtttcaaatg
      721 cggaatcgtg ggcctgccca atgtcggcaa gtccacttta tttaatgcat tgacgcaaac
      781 tgcggcagcg caggcggcca attatccgtt ctgcaccatt gagcccaatg tcggcgacgt
      841 ggcggtgccc gatccgcgtc ttgagacgct ggccgggatc gccggttcgc aggagatcat
      901 tcccacccgc atcacctttg tcgacatcgc cggcctggtg cgcggcgcct ccaagggcga
      961 agggctgggc aaccagtttc tcgccaatat ccgcgaggtc gatgccgtgg cgcatgtgct
     1021 gcgctgtttc atcgatgacg acatcaccca tgtcgaaggc aagatcgacc ccgtcgccga
     1081 tgccgagacg gtggaaaccg aactgatgct ggctgacctg gagagcctcg aaaagcggtt
     1141 gaagccgctg gagaaaaaag ccaacagcgg cgaaaaggaa gccaaggaac agttagcgct
     1201 gatgatgaag gcggtgacct tgctgcgcga aggcaagccc gcccgccgcg ccgatctggc
     1261 gccggaagaa cgcgcaccct attcccagtt gggcctgatg acggccaagc cggttctgta
     1321 tgtctgcaat gtcgaggaag gcagcgccgc caccggcaac gcgatttccg ccaaggtcga
     1381 agccatggcc aaagccgagg gcgcgcgcag cgtcgtgatc agcgccaaga tcgaggaaga
     1441 gatcgcgcaa atgccgatgg aagatcgcaa ggattttctc gacagcctgg ggctggaaga
     1501 gcccggcctg aaccggctga tccgcaccgg ctatgacctg ctgaatctgc tgaccttctt
     1561 cacagtcggt ccgaaggaaa cgcgcgcatg gaccgtgacc aggggctcaa aaggcccgca
     1621 ggcggcgggc gtgatccata ccgatttcga gcaaggctac atccgcgctg aaaccattgc
     1681 ctatcccgac tatgtcgcgg gcaagggcga agcgggcgcg cgcgaagcgg gcaagttccg
     1741 gctggaaggc aaggaatacg tcgtcgccga cggcgacgtc atgcatttta ggtttgcgaa
     1801 ctaaggtttg cggaaagagc tagaatcgca acatattggt gacagccata gacaacggat
     1861 ataggtcggt gtgctgccaa tttctgacga attacagagg caacgcctcg cagttcttgc
     1921 aacgatagaa acagaccctt atcgcagtgc aactacgata gggattgagg aagtgcttga
     1981 tgcgcactac ctaatagcag atctgtttgc agccagaggc cagagcttcg ttgccgttgg
     2041 gcccgctaga atagaacttc tgcattctgc actgcatcgt caattcgtag ttttaggcgg
     2101 ccgtcccaaa tggagcacgc cctcagaaat agctgcgacc gtgctttttg gcattgccaa
     2161 aaaccacgca ttctacgacg ccaacaaaag aacggcatta gtgtctgtct tatggctact
     2221 ttttgaaaaa tacaatctgt cgcccacggc ttctcaaaaa gcattggaag actttgtcgt
     2281 cgatgtagcc gacgatcagc ttgcagaaaa atacgcgcgc tgcaaagcaa gtaaacaatc
     2341 cggagaccct gatgccgaag ttcgcttcat cgcatattgg ataaaagaaa atacgagaaa
     2401 gatgcagaat gattatcgaa cgataacatt tagggaatta agaaagataa tgcgtcgata
     2461 taatttcgaa tttgaaaatc caaaagacaa ccaaatcgat atcgtcaacg tagaacagca
     2521 ttctggttta tttggaatcg gagcgagaga agttcgaaaa cgtgttggtc gtattggtta
     2581 tcactcggaa acaacgcaag ttgcaaaaag cgatattaaa aaacttcgtg aacgatgcgg
     2641 ccttacattc aaaagccata tggtcgattc cctggaattc tatgatgggg tcaatccaat
     2701 gaactttctt ctggccgagt atcaagacat actggttagt ctagcagacc gttagttacc
     2761 tcagtgaagc ctcgatattg ccgccgtcga ccgtgacgac agccgccgtc gtcttgctgg
     2821 cggtggcgag atagacaaaa gcgtctgcca cgtcttccgc cgtgacttcg cgcttcagaa
     2881 gattgcccgc catatagtcg ctttccgaca cgccgcgcgc cgcggaacgc ttggccacca
     2941 tgtcgtcggt gagcaggccg gaacggatgc ggtcggcgtt cacggcgttg gcgcggatgc
     3001 cgtccttgcc atggtccagc gcatattgct tgaccaggaa cagggtggcg gccttcggca
     3061 agccgtaggg gccgaaatcc ttgcccggat tgaccgcctg cttcgacgta ttgaacagca
     3121 gcacaccgaa cgtgccctgc gccagcatga tgcgggtggc gtgctgggcg acggcctggt
     3181 gggcccagaa attcaactcg aatgacttgc gcagggtctc gtcatcgaca tggccgatgg
     3241 tgccctgcca ggctgcgccg gcattcgaca ccacgatgtc cacgccgcca aaggcggaga
     3301 caacggcatc gaacgccttg cgaacgctca tcgggtcggt gacgtcgcac tggaccgcca
     3361 atgtcttgcc gccgatcttt ttcgcagcga cctttgccgc atccagatcg cggtcgagga
     3421 tggcgacttc ggcgccctgc ttcgccatgg ccttggcggt ggcggcgccg atgccgctgg
     3481 cgccgccggt gatgacagcg acctggcgcg acaacgcctt ttcgttcgac ttgcccagct
     3541 tggcctgttc cagcgaccag tattccactt cgaacatgtc gaattcgctg atcgagcggt
     3601 attcgcccat ggcttcggcg tcggtgatga cggcgacggc gttctcggcg atgtcggcgg
     3661 cgatggcgg
//
LOCUS       DASYCS010000418         5364 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4764021,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000418 DASYCS010000000
VERSION     DASYCS010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 5364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5364
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4764021"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VGM46_12830"
     CDS             complement(<1..255)
                     /locus_tag="VGM46_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193524.1"
                     /translation="MAELGRASPSKVAAQAGKAAPGASVHENPPSLTPVASAADHETA
                     RRKKAPAFAEAFDRLECLRSELVVYADANGVEVELPVKGSI"
     gene            complement(269..1327)
                     /locus_tag="VGM46_12835"
     CDS             complement(269..1327)
                     /locus_tag="VGM46_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY4193525.1"
                     /translation="MEKSDDKNSALFTDADARLAAIVDSSSDAIIGKDLNSTITNWNR
                     AAERLFGHTAEEAIGRSILILIPENRHAEEIDIIERVRRGERVETFDTMRLRKDGTQV
                     PVSLTISPIKNAAGEIIGASKIARDISQAKESERRIRLLMREVNHRVKNQFAVIISMV
                     RETAKRATNPQDFEKQMRDRIMALSRSHDLLVSADWQGASLFDLIEEHLKPFGHEEQI
                     TLSGPLLTLLPSAVQYLGMAFHELGTNSAKYGALADSAGRISVSWEIGPQAPDQRELS
                     IVWDETSAHTEQEEDGNHRGFGSVVLQRVTPASVNGMATLERSQGHVRWTLKAPASSA
                     IVQAGVEVDDDLPPLIAL"
     gene            1482..4160
                     /locus_tag="VGM46_12840"
     CDS             1482..4160
                     /locus_tag="VGM46_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEY4193526.1"
                     /translation="MRSGASAQLALTETVGHGIQAFARRQVGRLAGAALFGAVAFGIA
                     SLATWNVADPSFSHATDNIVTNAMGYPGAVFSDLAMQFFGLSAVAGLVPAVVWGFLLF
                     SARGVDKLPKRGLAWFGFALLAAAIAGCVVPPKTWPLPTGLGGVFGDMVLKVPGIFVG
                     GYPTGIIASVLAVLFVAPALWLFAYGSALIARRNGFAVMEPAVREQNDADQALFENED
                     DDESEGMLALGAITHWWLSLRAYMRRRAARRRERDGDDFDLEPAPRESAWRRAAERVE
                     VAEIAEARMSSNGRARVEPEFFAAMVNDRGNVAVHPDDADFFQEEDHYSDEDLGFAPA
                     PPVQRRAAPDANVQAYRSPAGAKIDAPASRPVEGTRVQREAQTSLIGADHFEMPSLHF
                     LLQPPSVVRDASLSKDALEQNARLLEGVLEDFGVKGEIIHVRPGPVVTLYELEPAPGI
                     KSSRVIGLSDDIARSMSAIACRVAVVPGRNAIGIELPNAKRETVYLREIMASRDFEQT
                     KAKLALALGKTINGEAVIVDIAKMPHVLVAGTTGSGKSVAINTMILSLLYKMTPQQCR
                     LIMIDPKMLELSVYDGIPHLLTPVVTDPKKAVVALKWTVREMEDRYRKMSKVGVRNID
                     GFNARVAQADKKGEKISRTVQTGFDRQTGEAIYETEALDLEPIPYIVVIIDEMADLMM
                     VAGKDIEGAVQRLAQMARAAGIHVIMATQRPSVDVITGTIKANFPTRISFQVTSKIDS
                     RTILGEQGAEQLLGMGDMLYMAGGGRIQRVHGPFVSDDEVEKIVAHLKLQGVPEYLDA
                     ITESDEDDDEETSGKGGSGGGNSNFDDSDDPYDQAVAVVLRDGKASTSYIQRRLGIGY
                     NRAASIIEKMEQEGIVGPANHAGKREILVPTEDDKF"
     gene            4369..4965
                     /locus_tag="VGM46_12845"
     CDS             4369..4965
                     /locus_tag="VGM46_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HEY4193527.1"
                     /translation="MNLAPSFLLLAQAQAAVPATAQKIADHFSSVKSMTGEFVQFGPK
                     GEQTGGKFYLERPGKIRFNYDGSSRFQVISDGESVVILNQKMRTSDLYPLSKTPLKLL
                     LDERIDLSGDKVKSVKEEDDLTTIQLADKSVFGNSRITMMFDPKTYELRQWTITDAQG
                     KDTTVMIFNTKEGVSFAADTFKIDYTANRELNTPTRSR"
     gene            5101..>5364
                     /locus_tag="VGM46_12850"
     CDS             5101..>5364
                     /locus_tag="VGM46_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018430287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEY4193528.1"
                     /translation="MPFTIATWNINSVRLRMPLVEKLLIEQAPDVLCLQETKCPDDLF
                     PFDAFRALGYGHIAIHGQKGYHGVATVARRPLEIVEKRRFCEIE"
BASE COUNT          973 a         1626 c         1753 g         1012 t
ORIGIN      
        1 gatgcttccc ttgaccggaa gttcaacttc cacaccgttc gcgtcggcgt aaactacaag
       61 ttctgatcga agacactcca gtcgatcgaa ggcctcggcg aaagccgggg ccttttttcg
      121 tctggccgtt tcgtggtccg cggccgatgc gaccggggtg aggctgggcg gattttcgtg
      181 gacagatgcg ccgggtgctg ctttgccggc ctgcgccgca accttgcttg gtgaagctcg
      241 gcccagctcg gccatctcgg agaggctgtt acagcgcgat tagcggcggc agatcgtcgt
      301 ccacttcgac gccggcctga acgatcgccg aggatgccgg ggccttcaat gtccagcgga
      361 catggccttg gctgcgttcg agcgtggcca ttccgttgac ggacgccggc gttacgcgct
      421 gcaagacgac actgccgaag ccgcggtgat tgccgtcttc ttcctgctcg gtgtgggcgg
      481 atgtttcgtc ccagactatc gaaagctccc gctgatcggg cgcttgcggg ccgatttccc
      541 agcttaccga aattcgcccg gcgctgtcgg ccagcgcgcc atatttcgcg gaattcgttc
      601 caagttcgtg aaaagccatg ccgagatact gcaccgcgct cggcagcagg gtcaaaagag
      661 gaccggacag ggtgatctgc tcttcatggc cgaatggctt caggtgctcc tcgatcaggt
      721 cgaacaggct tgcgccctgc cagtcggcgg ataccagcag atcgtgggac cgcgacaagg
      781 ccatgatgcg gtcgcgcatc tgtttttcga agtcctgcgg gttggtcgcc cgcttggcgg
      841 tctcgcggac catggaaatg atcacggcga actgattttt gacgcggtgg ttgacctcgc
      901 gcatcaacag gcggatgcgg cgttcgcttt ccttggcctg cgagatgtcg cgggcaatct
      961 tggaagcgcc gatgatctcg ccggcggcgt tcttgatcgg cgatatcgtc agcgataccg
     1021 gcacctgggt gccatccttg cgcaggcgca tggtgtcgaa ggtttccacc cgctcgccgc
     1081 gccggacgcg ctcgatgatg tcgatttcct cggcgtgccg gttttcgggg atgaggatca
     1141 gaatggaacg tccgatggcc tcttcggcgg tatggccaaa aagcctttcg gcggcgcggt
     1201 tccagttggt gatggtgctg ttgaggtctt ttccgatgat cgcgtcggaa gacgaatcga
     1261 cgatcgccgc aagccgcgcg tccgcatccg tgaagagggc cgaattctta tcgtctgatt
     1321 tttccatggc acggtttcta gcacaggtga tgaaccgatc aatctggcgt gcgaaagctc
     1381 gcatggttaa agcgccctta accttcctgc cgatagtctg acgaccgaat ctgccggacg
     1441 caagcgcgtc gcatcaggca atgacagacg aggaaggacg catgcgttca ggggcttcag
     1501 cacagcttgc gctgaccgag acggtggggc acggcatcca ggcgttcgcg cggcgtcagg
     1561 tcgggcggct cgcgggggcg gcgctgtttg gcgcggtggc cttcgggatc gccagcctgg
     1621 cgacatggaa cgtcgccgat cccagcttct cgcacgctac cgataacatc gtcaccaatg
     1681 cgatgggcta tccgggcgcg gtgttttccg atctcgccat gcagttcttc ggtctttccg
     1741 ccgttgccgg gctggtgccc gccgtcgtct ggggctttct tttgttttcg gcgcgcggcg
     1801 tcgataaatt gccgaaacgc gggttggcct ggttcggatt tgccttgctg gcggcggcga
     1861 tcgccggctg cgtcgtgccg ccgaagacgt ggccgctgcc gaccgggctt ggcggtgttt
     1921 tcggcgacat ggtgctgaag gtgccgggca tcttcgtcgg cggttatccg accggaatta
     1981 tcgcaagcgt gctggcggtg ctgttcgtcg cgccggcctt gtggctgttc gcctatggat
     2041 cggcgctgat tgcccgccgg aacggctttg ccgtgatgga accggcggtt cgcgaacaaa
     2101 acgacgccga ccaggccttg ttcgagaacg aggatgacga cgagagcgaa ggcatgctgg
     2161 cgctgggggc gatcacccat tggtggctgt cgctgcgcgc ctatatgcgc cgccgcgccg
     2221 cacggcggcg ggaacgcgac ggcgacgatt tcgaccttga gccggcgccg cgcgaaagtg
     2281 cgtggcggcg cgccgccgag cgcgtggaag tggccgaaat cgccgaggcg cgaatgagtt
     2341 cgaacggccg cgcgcgcgtc gagccggagt ttttcgcggc gatggtgaac gatcgcggca
     2401 acgtggcggt tcatcccgac gacgctgatt tcttccagga agaggaccat tacagcgacg
     2461 aagatttggg cttcgcgccc gcgccgccgg tccagcgccg cgccgcgccc gatgcgaatg
     2521 tgcaggccta tagatcgcca gccggggcca agatcgatgc gccggcttcg cggccggtgg
     2581 aaggcacgcg cgtccagcgc gaggcgcaga catcgctgat cggcgccgat catttcgaga
     2641 tgccgtcgct gcatttcctg ttgcagccgc caagcgtggt gcgcgacgcc agcctgtcca
     2701 aggacgcgct ggagcagaat gcgcgcctgc tggagggcgt gctggaggat ttcggcgtca
     2761 agggcgagat cattcatgtc cgccccggtc cggtggtcac gctttacgag ctggagccgg
     2821 cgcccggcat caagtcatcg cgcgtcatcg gcctgtccga cgatatcgcc cgctcgatga
     2881 gcgccatcgc ctgccgcgtt gcggtggtgc cggggcgcaa cgccatcggc atcgaactgc
     2941 ccaacgccaa gcgcgagacg gtttacttgc gcgaaatcat ggccagccgc gacttcgagc
     3001 agaccaaggc caaactcgct ttggcgctgg gcaagaccat caatggcgag gcggtgatcg
     3061 tcgacatcgc caagatgccg catgtgctgg ttgccggcac caccggttcg ggcaagtcgg
     3121 tggccatcaa tacgatgatc ctgtcgctgc tctacaagat gacgccgcaa cagtgccgcc
     3181 tcattatgat cgacccgaaa atgctcgaac tttccgtcta tgacggcatt ccgcaccttc
     3241 tgacgccggt ggtgaccgat ccgaagaagg ccgtcgtcgc gctcaaatgg acggtgcgcg
     3301 agatggagga ccgttaccgc aagatgtcga aggtcggggt gcgcaacatc gacggcttca
     3361 atgcccgcgt cgcgcaggcc gacaagaagg gcgagaagat ttcgcgcacc gtgcagaccg
     3421 gcttcgaccg gcagaccggc gaggccatct acgagacgga agcgctcgac cttgagccga
     3481 tcccctatat cgtcgtcatc atcgacgaga tggccgacct gatgatggtg gccggcaagg
     3541 atatcgaagg ggcggtgcag cggctggcgc agatggcgcg cgccgccggc atccacgtca
     3601 tcatggcgac gcagcgccct tcggtcgatg tcatcaccgg cacgatcaag gccaacttcc
     3661 cgacgcgcat ctccttccag gtgacgtcga agatcgacag ccgcaccatt ctgggcgagc
     3721 agggcgcgga gcagctgctc ggcatgggcg acatgctgta catggccggc ggcggccgca
     3781 tccagcgcgt ccacggcccg ttcgtgtcgg atgacgaggt tgaaaagatc gtcgcgcacc
     3841 tgaagcttca gggcgtgccg gaatatctcg acgccatcac cgagagcgac gaggacgacg
     3901 acgaggaaac gtccggcaag ggcggttcgg gcggcggcaa cagcaatttc gacgattcag
     3961 acgatcctta cgaccaggcg gtggccgtgg tgctgcgcga cggcaaggcc tcgaccagct
     4021 acatccagcg ccggctcggc atcggctata accgcgccgc ctcgatcatc gagaagatgg
     4081 agcaggaagg cattgtcggc ccagcgaacc atgcgggaaa acgcgagata ctggtgccga
     4141 cggaggacga caagttttag gcctttgcgg cggccgctcg ccgctggatg ggaaccttgc
     4201 cgggacacat gggtttagca gcggctcgcc aaactaaagc ccaactgggc ggccagattc
     4261 aagccagact ggaatcagac gaaagcgaca ggcatgaccc attcttcctt tgcgcccacc
     4321 cgccgccagc tgcttggcct ggggctgacg ctcgcagggg ctgccgcgat gaacctggcg
     4381 cccagctttc tgctgctggc gcaggcgcag gcggcggttc ccgccaccgc gcagaagatc
     4441 gccgaccact tttcctcggt gaagtcgatg accggcgaat tcgtgcagtt cggccccaag
     4501 ggcgagcaga ccggcggcaa attctatctg gagcgtccgg gcaagatacg gttcaactat
     4561 gacggctcgt ccaggttcca ggtgatttcg gatggcgaat cggtggtgat cctcaaccag
     4621 aagatgcgga cttccgacct ctacccgctt tcgaaaacgc cgctcaagct gctgctcgac
     4681 gagcggatcg acctttccgg cgacaaagtg aagagcgtca aggaagagga cgacctgacg
     4741 acgatccaac tggccgacaa gtcggtgttc ggcaattcga ggatcaccat gatgttcgat
     4801 ccgaaaacct acgaactgcg ccagtggacc atcaccgacg cgcagggcaa ggatacgacg
     4861 gtaatgatct tcaataccaa ggaaggcgtc agcttcgccg ccgacacgtt caagatcgac
     4921 tataccgcca accgcgaact caacacgccg accaggtcgc gttgatattt ttcgcttggc
     4981 ggcgctggct gcgccgatag ttgtggaaag cccggtgcgc cttgcgtgat gcagccacgc
     5041 ggcttgtttt cagccgcaca ggctggcagg ttccgcgcaa acttgcgccg gacaccagcc
     5101 atgcccttca ccatcgccac ctggaacatc aattcggtgc ggctgcgcat gccgctggtc
     5161 gagaagcttt tgattgagca ggcgccggat gtgctgtgcc ttcaggaaac caagtgcccg
     5221 gacgatcttt tcccgttcga cgccttccgg gctctcggct acgggcacat cgccattcat
     5281 ggccagaagg gctatcacgg cgtggcgacg gtggcacggc ggccgcttga aatcgtcgag
     5341 aagcggcgct tttgcgaaat cgag
//
LOCUS       DASYCS010000419         7413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_559723,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000419 DASYCS010000000
VERSION     DASYCS010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 7413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7413
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559723"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="VGM46_12855"
     CDS             <1..849
                     /locus_tag="VGM46_12855"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HEY4193529.1"
                     /translation="VKQLKLADTRKIEAPYGVLPDLKQIDFSRDVVFTWNGTTSGVRV
                     PNAGFIAADRQGLTICDATSAAFAQKLDFEKLDVVTFSWQKALGGEGAHGMLILSPRA
                     VARLESYKPAWPLPKIFRLTSNGKLIEGIFKGETINTPSMLCVEDYLDALIWAKDIGG
                     LDALVARADKNAAVIDRFVQKSSWLGHLAADEASRSNTSVCLSIVDPEITALDADGQA
                     AFAKRLVSALDKEGVAFDIGHYRDAPPGLRIWCGATVEAADLEALMPWLDWAFSQQKA
                     ALKQAA"
     gene            997..2589
                     /gene="serA"
                     /locus_tag="VGM46_12860"
     CDS             997..2589
                     /gene="serA"
                     /locus_tag="VGM46_12860"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006327747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEY4193530.1"
                     /translation="MPRVLVSDKLSPTAVQIFKDRGVEVDYLPDLGKDKEKLLEVIGQ
                     YDGLAIRSATKVTEKLIAAAKNLKVIGRAGIGVDNVDIPAASRRGIIVMNTPFGNSIT
                     TAEHAVAMIFALARQIPEANASTHAGKWEKNRFMGTEITGKTLGIIGCGNIGSIVAAR
                     GVGLKMHVIAFDPFLSDKRAEELGVDKVELDELFARADFITLHTPMTEKTKGIINADA
                     IAQMKDGVRIINCARGGLVVEADLIAALKSGKVAGAGIDVFETEPATESPLFGMENVV
                     ATPHLGASTAEAQENVALQVAEQMSDYLIKGAVTNAINMPSITAEEAPRLKPFVKLAE
                     VLGAFVGQVTEDPITEVEILFDGSTATMNTRALISAALAGLIRPQVSDVNMVSAPIMV
                     KERGIIVAEVKRDKSGVFDGYIKVTVTTEKMTRSIAGTCFSDGKPRFIQIKGINLDAE
                     VGAHMLYTTNADVPGIIGLLGTLCGENNVNIANFQLGRDHQGGDAIALLYLDAPFPEN
                     VLAQVRSNKSIDSAKRLQFDVA"
     gene            complement(2710..3957)
                     /locus_tag="VGM46_12865"
                     /pseudo
     CDS             complement(2710..3957)
                     /locus_tag="VGM46_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010660046.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
     gene            complement(4063..5112)
                     /locus_tag="VGM46_12870"
     CDS             complement(4063..5112)
                     /locus_tag="VGM46_12870"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEY4193531.1"
                     /translation="MSKENACFRPDDLRRWISSVLQSQGVAAKDADETARVLIRTNLR
                     GIDTHGIGRVSGYVDKLRSGELNARPDGSMELRDGVLYVDGDGGLGQSVGMKAVHEAA
                     ARAAQTSVVNCFIRRSGHLAAIGQYALEAAERGMVAMICQETPPLMALEGSSRPAIGN
                     NPIAFACPVKGQAPLVFDMATSVVARGNLLQAISDGKTTIPDGWAIGPDGKPTNDPQL
                     ALQGAMRPVAGHKGVGIAMMVQVLAGSLTGSLTADSAATHGATSSAGNVSAFLMVINP
                     QSVIGREAFDAHVAQWIGTYLSASGEGSRYPGQRASETEAIRETSGIPIPASTFEQLR
                     KVGFSVGVPFEPRTI"
     gene            complement(5150..6742)
                     /locus_tag="VGM46_12875"
     CDS             complement(5150..6742)
                     /locus_tag="VGM46_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020043989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HEY4193532.1"
                     /translation="MTDMNFSFSYPSQRSPLLARNVVSTSQPLAAQAGLQMLAKGGNA
                     VDAALAAAMVLIVTEPTGNGLGGDAFAIIWDGKQLHGLNASGRAPEGWTEQRFAGHDK
                     MPDRGWESVTVPGMVSAWVELSQKFGRLPLEELAKPAINYARNGFAVSPVIATLWGER
                     AAAVQDQPGFRECFMPNGRSPKAGEIFSSQDMANSIELIARTKGEAFYRGELAEKIAA
                     YAKQHDAALSLDDLNRHRLDWCGTASVGFQDVQLHEIPPNGQGISALMALGILSNTDV
                     ARYEPDSAEALHLQIEAMKIAFADANRYVSDPDHMPFSVEELISADYLKGRAQEINPD
                     RAGTPHARAPRDSGTVYVTAADDSGMMVSYIQSNFNGFGSGVVIPGTGISMQNRGLSF
                     VTEAGHPNRVGPRKRPFHTIIPGFVMRGDQPLMSFGVMGGPMQSQGHVQMLLRTQLWK
                     QNPQLASDAPRWRVLDGLKVAVESTMNESAIEGLKARGHIIQPERVDTAFGFGGAQLI
                     QRIEGGYIAGSDHRKDGFAVGF"
     gene            complement(6800..7195)
                     /locus_tag="VGM46_12880"
     CDS             complement(6800..7195)
                     /locus_tag="VGM46_12880"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY4193533.1"
                     /translation="MASTNIKSVNAEGLSPPGGHYSHVNIAGNLAFLSGQLPIDPSGK
                     VLSDQPFKDQVQRVLDNIDACLRQIGASRSSLVQIRVFVTDMEHWKDFDILYKEWIGE
                     HRPSRAVVGASELHFGTLIEVEAVAVISG"
     gene            complement(7233..>7413)
                     /locus_tag="VGM46_12885"
     CDS             complement(7233..>7413)
                     /locus_tag="VGM46_12885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-5'-phosphate-dependent protein subunit
                     beta"
                     /protein_id="HEY4193534.1"
                     /translation="ATRELAAMGFYCEPTSAVSAAAIAQLTEQGFIQPGQSTVVVLTG
                     SGLKTSASMDQIFAS"
BASE COUNT         1341 a         2416 c         2308 g         1348 t
ORIGIN      
        1 gtcaagcagc tcaagcttgc cgacacgcgc aagatcgaag cgccctatgg cgtgctgccg
       61 gacctgaagc agatcgattt ctcgcgcgac gtggtgttca cctggaacgg caccacctcc
      121 ggcgtgcgtg tgccgaacgc cggtttcatt gcggccgacc gccagggcct gaccatctgc
      181 gacgccactt cggcggcttt cgcgcagaag ctggatttcg aaaagctcga tgtcgtcacc
      241 ttctcctggc agaaggcgct gggcggcgaa ggcgcgcacg gcatgcttat cctgtcgccc
      301 cgcgccgtcg cgcggctgga aagctacaag ccggcatggc ctctgcccaa gattttccgc
      361 ctgacctcca acggcaagct gatcgaaggc atcttcaagg gcgaaaccat caacacgccc
      421 tcgatgctat gcgtcgagga ctatctcgac gcgctcatct gggcgaagga cattggcggg
      481 ttggacgccc tcgtcgcccg cgccgacaag aacgccgcgg tgatcgaccg cttcgtgcag
      541 aaatcgtcat ggcttggcca tctggcggcc gacgaggcat cgcgctccaa cacctcggtc
      601 tgcctgtcca tcgtcgatcc cgaaatcacc gcgctcgatg ccgacggcca ggcggccttc
      661 gccaagcggc tggtgtcggc gctcgacaag gagggcgtgg ccttcgacat tggccattac
      721 cgcgacgccc cgcccggcct tcgcatctgg tgcggcgcga ccgtcgaggc tgccgacctc
      781 gaagcgctga tgccgtggct cgactgggct ttttcccagc aaaaggccgc gctgaaacaa
      841 gcagcataac gctcttaccc tccccgttgt ggggggggtc ggaccgcagg tccgggtggg
      901 gtacgccccc accggcattt caccatttcc ccagccggtc gcattgcggc cgacctcccc
      961 gcaaggggag ataggctaat cgcaaaggat attcccatgc cccgcgttct cgtttccgac
     1021 aaactctccc ccaccgctgt gcaaatcttc aaggatcgcg gcgtcgaggt cgattatttg
     1081 cccgatctgg gcaaggacaa ggaaaagctg ctcgaagtga tcggccagta tgacggcctg
     1141 gccatccgct cggccaccaa ggtcaccgaa aagctgattg ccgcggcgaa gaacctgaag
     1201 gtcatcggcc gcgccggcat cggcgtcgat aatgtcgaca ttcccgccgc ctcgcggcgc
     1261 ggcatcatcg tcatgaacac ccctttcggc aattccatca ccaccgccga acatgcggtc
     1321 gccatgatct tcgcgctggc ccgccagata ccggaggcca acgcgtccac ccatgccggc
     1381 aagtgggaga agaaccgctt catgggcacc gagatcaccg gcaagacgct gggcatcatc
     1441 ggttgcggca atatcggctc gatcgtggcg gcgcgtggcg tcggcctgaa gatgcatgtc
     1501 atcgccttcg acccgttcct gtccgacaag cgcgccgagg aactgggcgt cgacaaggtc
     1561 gagctggacg agttgttcgc ccgcgccgat ttcatcaccc tgcatacgcc gatgacggaa
     1621 aagaccaagg gcatcatcaa tgccgacgcg atcgcgcaga tgaaggacgg cgtgcgcatc
     1681 atcaactgcg cgcgtggcgg gctggtcgtc gaggccgacc ttatcgcggc actgaagagc
     1741 ggcaaggtgg cgggcgccgg catcgatgtc ttcgaaacag agccggctac ggaaagcccg
     1801 cttttcggca tggaaaacgt ggtggcgacc ccgcatctcg gcgcttcgac ggccgaagcg
     1861 caggaaaacg tcgcgctgca agtggccgag cagatgtccg actacctcat caagggcgcg
     1921 gtcaccaacg ccatcaacat gccgtcgatc acggccgagg aagcgccccg gctgaagccc
     1981 ttcgtcaagc tggccgaagt gcttggcgcg ttcgtcggcc aggtgacgga agacccgatc
     2041 acggaagtcg aaatcctgtt cgacggctcg accgcgacca tgaatacccg cgccctcatc
     2101 agcgccgcgc ttgccggcct gatccggccg caggtgtcgg acgtcaacat ggtgtcggcc
     2161 cccatcatgg tgaaggagcg cggtatcatc gtcgccgagg tcaagcgcga caagtcaggc
     2221 gtgttcgacg gctacatcaa ggtgacggtg accacggaaa agatgacgcg ctccatcgcc
     2281 ggcacctgct tctccgacgg caagccgcgc ttcatccaga tcaagggcat caacctcgac
     2341 gccgaggtcg gcgcccacat gctctatacg accaatgccg acgttcccgg cattatcggt
     2401 ctgctcggca cgctgtgcgg cgaaaacaac gtcaacatcg ccaacttcca acttggccgc
     2461 gaccatcagg gcggcgatgc catcgccctg ctctatctcg atgcgccttt tcccgaaaat
     2521 gttctggcgc aggtgcgttc gaacaaatcg atcgattcgg caaagcggct acagttcgat
     2581 gtagcctgaa gacaggtggc gcgggccttc gggtccgcgc aacctgcgcc ggttaagtaa
     2641 tatttgcggg cttgttgaag acgcgcatcg tcaggcctgc gaggacatgc ccagttcaag
     2701 gcttgcctga taggcggtca gatcggtaat cgcacggcgc gcaacgccgc tgtccatcgc
     2761 cgccttcgca accgccggcg cgatccgcag gatcagccgg ggatcgaaag gcgacgggat
     2821 aagccgggtg cgatccagcg tcaccggccc ggcagccgtc ggcggctcaa gtccaggttc
     2881 gcgcgcaagc tgggcgatcg cctccacagc cgcccgtttc atcggctcgt tgatcgccgt
     2941 cgcctcgaca tcgagtgcac cccggaaaat gaacggaaaa cacagcacgt tgttgacctg
     3001 gttgggatag tccgaccggc cggtgcagat cagcgcgtcg ggccgctccg caatcgccag
     3061 ctccggcata atttcgggaa cagggttgga cagagcgagg atcaacggat cgcgcgccat
     3121 gccgcgtatc atctccggct tgagtgcgcc cgcctgcgac aggccgacat agatatcggc
     3181 gccctccatc acatcggcca gatgggtagc ctcggtttcc tgcgcgaagg caccgcgcca
     3241 gcggtcaacc gttgcgatgc gctttgtcgt gaccagcccg ccccggtcgg cgatccagat
     3301 gttttcgcgc ctggcgccaa gctcaagcag catatccatg caggcaatgg ccgccgcacc
     3361 ggcacccgac gtgcatatct tgaccttggc aatatccttg ccgatcaggt gcagcgcatt
     3421 gacgaccgcc gccgcaacga tgatcgcggt gccatgctgg tcgtcgtgaa agaccgggat
     3481 gttcatgcgc gcgcgcagcg cctcctcgat ctcgaaacag tccggcgcct tgatgtcttc
     3541 gaggttgatg ccgccgaagc ttggctcaag cggcgcgacg atatcgacga agcgcttcgg
     3601 atcggtctcg tcgacctcga tatcgacgct gttgatcccg gcgaatttct tgaaaagcac
     3661 cgccttgcct tccattaccg gcttgctggc aagcgccccg atattgccga ggccgagtac
     3721 cgccgtgccg ttcgagatga cagccacgag gttgcccttc gacgtgtagc gataggcctg
     3781 cgccgggtcg gcggcaatct cctcgcaagg cgcggcaacg cccggagaat aggcgagcgc
     3841 caggtcgcgc aggctggcca gcggcttggt cggcgtcatc tcgattttgc cgggcttggg
     3901 atattcatgg aaatgcaagg cagcggcgcg caagtcggat tgagtttcct gggacaaaat
     3961 gcggtttcct cctggtttgc tgatgattgc ggacgcaaat ggccccagcg gtccggcttc
     4021 ccggaccgct gccgatgctc cgtgacggga tgcggttacg cttcagatgg ttcggggttc
     4081 gaacggcacg ccgaccgaaa aaccgacctt tcgaagctgt tcgaacgtgc tggcgggaat
     4141 cggaatgccg gaagtttccc ggattgcctc cgtttcgctg gcgcgctggc cgggatagcg
     4201 gctgccttcc ccgctcgccg acagataagt tccgatccat tgcgcgacat gggcgtcgaa
     4261 ggcttccctg ccgatgaccg attgcggatt gatgaccatc aggaacgcgc tgacattgcc
     4321 ggcgctgctg gtcgccccgt gggtcgcggc gctgtcggcg gtcagcgagc cggtcaggct
     4381 gccggccaga acctgcacca tcatggcgat gccgacgccc ttgtggccgg ccacggggcg
     4441 catcgcgccc tgaagggcca gctgcgggtc gttggtcggc ttgccgtccg gcccgattgc
     4501 ccagccgtcg ggaatcgtgg tttttccgtc gctgattgct tgcagcagat tgccgcgcgc
     4561 caccacgctg gtcgccatgt cgaacaccag cggcgcttgc cctttcaccg ggcaggcaaa
     4621 cgcaatcggg ttgttgccga tggccggtcg tgacgatccc tccagcgcca tcagcggcgg
     4681 cgtctcctgg cagatcatcg ccaccatgcc gcgctcggct gcctctaatg catattgccc
     4741 gatggccgcc agatggccgc tgcgtcgtat gaagcagtta accacgctcg tttgcgccgc
     4801 gcgcgccgcc gcctcatgga cagccttcat gccgaccgac tgtccaagcc cgccatcgcc
     4861 gtccacgtaa agcacgccgt cgcgcagttc catgctgcca tctggcctgg cgttcagttc
     4921 cccgctgcgc agcttgtcga catagcccga aacgcggccg atgccgtgcg tgtcgatgcc
     4981 gcgcagattg gtgcggatca gcacgcgcgc ggtctcgtcc gcatccttgg cggccacgcc
     5041 ctgcgattgc aggaccgacg aaatccaccg cctgagatcg tcggggcgga aacatgcatt
     5101 ctctttggac atcggtgctc tcggctggag gaatgaagcg gccttaaggc tagaagccga
     5161 cggcgaagcc atccttgcgg tggtcggaac cggcaatata gccgccctcg atgcgctgga
     5221 tcagctgcgc gccgccgaag ccgaaggccg tgtcgacgcg ctcgggctgg atgatatggc
     5281 cgcgcgcctt gaggccttcg atggcgcttt cgttcatcgt gctttccacc gccaccttga
     5341 ggccgtccag cacgcgccag cgtggagcgt cgctcgccag ctgcgggttc tgcttccaca
     5401 attgggtgcg cagcagcatc tggacatggc cctgcgactg catcggcccg cccatcacgc
     5461 cgaagctcat cagcggctgg tcgccgcgca tgacaaagcc cggtatgatc gtatggaacg
     5521 ggcgcttgcg cggtccgaca cggtttggat gcccggcctc cgtcacgaag ctcaagccgc
     5581 gattctgcat gctgatgcca gtgcccggaa tgacgacgcc cgagccgaag ccgttgaaat
     5641 tcgactggat gtacgaaacc atcatgccgc tgtcgtcggc ggccgttaca tagacggtgc
     5701 cgctgtcgcg cggcgcgcgc gcatgcggcg tgccggcgcg gtcggggttt atttcctgcg
     5761 ccctgccctt cagatagtcg gccgaaatca gttcctcgac cgagaacggc atgtggtcgg
     5821 ggtcgctgac atagcggttg gcatcggcaa aggcgatctt catcgcctcg atctggagat
     5881 gcagcgcctc ggcgctgtcg ggttcgtagc gtgcaacatc ggtgttgctg aggatgccaa
     5941 gcgccatcag cgccgaaatg ccctgcccgt tcggcgggat ttcatggagc tgcacatcct
     6001 ggaagccaac cgaagcggtg ccgcaccaat ccagccggtg ccggttgaga tcgtcgaggc
     6061 tcagcgccgc atcatgctgc ttggcatagg cggcgatctt ctcggccagt tcgccgcgat
     6121 agaacgcctc gcccttcgtc ctggcgatga gttcgatgct gttggccata tcctggctcg
     6181 aaaaaatctc gccggctttg ggcgaacgtc cattcggcat gaaacattcg cggaagcccg
     6241 gctggtcctg cacggccgcc gcccgctcgc cccaaagcgt tgcgatgacg ggggaaacgg
     6301 caaaaccgtt gcgcgcatag ttgatcgccg gcttggccag ttcttccagc ggcaagcgcc
     6361 cgaatttctg cgacagctcc acccaggccg acaccatgcc gggaacggtg acgctttccc
     6421 agccgcggtc cggcatcttg tcatgcccgg cgaaacgctg ctcggtccag ccctccggcg
     6481 cccggcccga cgcgttgagg ccgtgcaact gcttgccatc ccagatgatg gcgaacgcgt
     6541 cgccgcccag tccgttgccg gtcggctccg tgacgatcag caccatggcg gcggcaaggg
     6601 ctgcatcgac ggcgttgccg cccttggcca acatctgcaa gcccgcctgc gcggcaagcg
     6661 gctgcgaggt cgagacgaca ttgcgcgcca aaagcggcga gcgctgcgag ggataggaaa
     6721 agctgaaatt catgtcggtc atgtcaggtc actccgccgg tatggcgctg agggaaagcg
     6781 gttgaggttg gatggaggat cagccggaaa taacggcgac ggcctcgact tcgatcagcg
     6841 tgccgaagtg aagctcgctg gccccgacca cggcgcgcga cggccgatgc tcgccgatcc
     6901 attccttgta gaggatgtcg aaatccttcc agtgctccat gtcggtcacg aacacgcgaa
     6961 tctgcaccag cgacgagcgc gacgcgccga tctggcgcag gcaggcgtcg atattgtcga
     7021 gaacacgctg gacctgatcc ttgaaaggct ggtccgacag caccttgccc gacggatcga
     7081 tcggcagctg gcccgaaaga aaggcgaggt ttccggctat gttgacatgc gaataatgcc
     7141 cgcccggcgg cgaaaggccc tcggcattga cgcttttaat gttcgtggaa gccacttgtt
     7201 tctccagtct tcagcgctcg cgcaggcgca tgttatgacg cgaatatctg gtccatgctc
     7261 gcgctggtct tgaggcccga accggtcagc accaccaccg tggattgccc cggctggatg
     7321 aagccctgtt cggtaagctg cgcgatggcg gcagcggaaa ccgcgcttgt cggctcgcag
     7381 tagaacccca tcgccgccag ctcccgcgta gcc
//
LOCUS       DASYCS010000420         2373 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2156668,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000420 DASYCS010000000
VERSION     DASYCS010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2156668"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VGM46_12890"
     CDS             complement(<1..311)
                     /locus_tag="VGM46_12890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193535.1"
                     /translation="MAASRPRKKKSPWLDALRRRLRGVVTTINIVEDRMTRERILAGG
                     VAAAILVAAAAFVVAPGSDANAQQKRPAPLDLGPNAASTPWTRYPGWPARDMSKFNTL
                     A"
     gene            complement(268..591)
                     /gene="soxZ"
                     /locus_tag="VGM46_12895"
     CDS             complement(268..591)
                     /gene="soxZ"
                     /locus_tag="VGM46_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003597861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HEY4193536.1"
                     /translation="MAEKPRLKLPKEAKKGEIIEIKTLIPHVMLTGLTKDKDGKTIPR
                     KIINKFTAEFNGKPVFSASIEPAVAANPYLQFTAKVEESGTFKFSWTDDDGSVTTAEE
                     KITVA"
     gene            complement(640..1095)
                     /gene="soxY"
                     /locus_tag="VGM46_12900"
     CDS             complement(640..1095)
                     /gene="soxY"
                     /locus_tag="VGM46_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HEY4193537.1"
                     /translation="MRKLTRRAALAVGAGAVTLGVVGWSQGAQAAAKEAADEVAKFTG
                     GKEATKGKITIELPEIAENGNTVPLTVSVDAPMTAENYVSDVLVVSEGNPNPGVVTFH
                     FTPLSGKAEAQTRIRLAATQNIVVVAKTSKGEFYTGQKQVKVTIGGCGG"
     gene            complement(1235..1828)
                     /locus_tag="VGM46_12905"
     CDS             complement(1235..1828)
                     /locus_tag="VGM46_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEY4193538.1"
                     /translation="MTFTRRTLLVGAAGLGTSLGSGLAGLGAARADEPILTDDGLYKQ
                     PWFLESFLDLSDDLEGAAKEGKRFAIMWELKGCPYCKETHFVNFAQPKISDYIKNNFE
                     ILQLNIIGSRKVTDFDGAEMSEKELAAKYGVRFTPTFQFFGERAAVLKPLAPAKREVA
                     RAPGYLRPEDFLAMFRFVKEKAYETKSFRDFVKSLPS"
     gene            1896..>2373
                     /locus_tag="VGM46_12910"
     CDS             1896..>2373
                     /locus_tag="VGM46_12910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193539.1"
                     /translation="MWRCWALGTTLGAILFLVIAVAPAKAGDLADFNDLMEGVTSHIR
                     VALGYLRTGNTDLASLEIDRTREAWGKLHLHFGSRRPEMFETSPLYKKIVTGVSARLI
                     GADLLLNAGRPEAALDSLNVLRGEFYDLRKGSGIVVLADCIRDASTAMDALMTYNDR"
BASE COUNT          451 a          714 c          700 g          508 t
ORIGIN      
        1 ttggcaagcg tgttgaactt cgacatgtcg cgagccggcc agccggggta acgcgtccat
       61 ggcgtcgaag cggcattcgg gcccaaatcg agaggcgcgg gccgcttctg ctgggcattc
      121 gcgtcagaac cgggtgcaac aacaaaagcg gccgccgcga ccaatatcgc tgccgccacg
      181 ccgcctgcca aaattcgttc gcgcgtcatc cgatcctcca cgatatttat tgtcgtcact
      241 acgccgcgta atcggcggcg cagcgcatca agccacggtg attttttctt ccgcggtcgt
      301 gacgctgcca tcgtcgtcgg tccaggagaa cttgaaggtg ccgctctcct ccaccttggc
      361 ggtgaactgg agatatggat tggcggcgac ggccggttcg atgctcgccg agaacaccgg
      421 cttgccgttg aactcggcgg tgaacttgtt gatgatcttg cgcgggatgg tcttgccgtc
      481 tttgtccttc gtcagaccag tcaacatgac atgcggtatc aacgtcttga tctcgatgat
      541 ctcgcccttc ttggcttctt tgggtagttt gagccgcggt ttttccgcca tcatggtctc
      601 ctgaagctct gaaattcaaa atcggaaacg ggaaagaaat cagccgccgc agccgccgat
      661 ggtgaccttg acctgcttct gcccggtgta gaattcgccc ttactggttt tcgctaccac
      721 gacgatgttc tgcgtcgccg caagccggat acgggtctgt gcttcggcct tgccggacag
      781 tggcgtgaag tggaaagtca caacgcccgg gttcggattg ccctcagaga cgaccagtac
      841 atcgctgaca taattctcgg cggtcatcgg cgcgtcgacc gaaacggtca gcggcacggt
      901 gttgccgttc tcggcaattt ccgggagctc gatggtaatt ttgcctttgg tggcttcctt
      961 gccgcccgtg aacttggcaa cttcgtcggc ggcttccttg gccgcggcct gcgcgccctg
     1021 gctccagccg acgacgccaa gcgtcacggc accggcgcca acggccagcg cagcgcgacg
     1081 ggtgagtttc cgcatcgaga cttcctcccg aatcaatatg taggagccaa ccgccccagt
     1141 cgcgccgaac atagccacga gaggttacgg gttcaaatcg tattcattaa aaagaatata
     1201 catgctgttg cactgcacac acgcagcata aaattcagct tggcagggat ttgacgaagt
     1261 cccggaaact ctttgtctcg taggcctttt ccttgacgaa gcgaaacatc gccaggaagt
     1321 cctccggccg caggtaaccg ggcgcgcggg cgacctcccg cttggccggt gcaagcggtt
     1381 tcaggaccgc cgcgcgttcg ccgaaaaact ggaaagtcgg cgtaaaccgc acgccgtatt
     1441 tggccgccag ctccttctcc gacatctcgg caccgtcgaa atcggtgact ttgcgggagc
     1501 cgatgatgtt gagctgcagt atttcgaagt tattcttgat gtagtccgag attttcggct
     1561 gggcgaaatt gacgaaatgc gtctccttgc aataggggca gcccttgagc tcccacatga
     1621 tcgcgaagcg cttgccttcc ttggccgcgc cttcgagatc gtcgctaagg tccagaaaac
     1681 tctccagaaa ccacggctgc ttgtagagcc cgtcatcggt cagaatgggc tcgtcggcgc
     1741 gcgcggcgcc caggccggcc aggccggagc ccaaacttgt gcctaaacct gccgccccta
     1801 ccagtagtgt gcgccgggtg aatgtcatgg tcttagaagt atccatgctc gcgcccgagg
     1861 tctatatcct cggcccgcaa ttaaggtgat caccgatgtg gcgatgctgg gcgcttggta
     1921 caactcttgg cgcaattctc tttctcgtga tcgcggtggc gcccgccaaa gccggcgatc
     1981 tcgctgactt caacgacctg atggaggggg ttacgagcca tattcgggtc gcgctcggct
     2041 atctcaggac cgggaatacc gatcttgcgt ctctggaaat cgatcgtacc cgggaggcct
     2101 ggggcaagct gcacctgcac ttcggcagcc ggcgcccgga gatgttcgag acgagcccgc
     2161 tgtacaagaa aatcgtgacc ggcgtgagcg cgcgcctgat cggtgccgac ctgctgctca
     2221 atgccggacg tccggaagcc gcgctggatt ccctgaacgt attgcgtggc gaattttatg
     2281 acttgcgcaa aggcagcggc atcgtggtgc tggccgactg catccgcgac gccagcacgg
     2341 caatggacgc gctgatgacc tacaacgacc gcg
//
LOCUS       DASYCS010000421         2662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_998945,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000421 DASYCS010000000
VERSION     DASYCS010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_998945"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="VGM46_12915"
     CDS             <1..572
                     /locus_tag="VGM46_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018428579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEY4193540.1"
                     /translation="AAIDDRRRIGFSRFLYALGIRHIGDTNAKRLARHFLSFQALREA
                     GESAVMPQGKGDPGNAAWQELNGINGIGGVVAEALVDFFGEEHNRTVVDALLAEVTPL
                     DEEPVGGVDSPVAGKTVVFTGALEKMSRDEAKAMAEKLGAKVAGSVSKKTDLVVAGPG
                     AGSKLKLASELGIEVIDEDAWFTRIGRVG"
     gene            575..1264
                     /locus_tag="VGM46_12920"
     CDS             575..1264
                     /locus_tag="VGM46_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013530968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02453 family protein"
                     /protein_id="HEY4193541.1"
                     /translation="MDSFKGFGEKAFPFLKALDFHQSREWFVENKDLFETELREPLGD
                     LVDTLTVRFEAAGIGLKGDHKKSLFRINRDVRFAKDKRPYNRHVSAILSPDGSKMEQG
                     VFFVHIGLERCFAAIAWWGVAPELLLAMRRAIADRPGDFRAMTAALKRNGLELGSEGA
                     MKRMPRGFEQVGDADLAAAIRNRHFVVRQEIDPAGIHTAALADELYGFVQRARPLLEW
                     GRAIEGKTAAQ"
     gene            1367..2104
                     /locus_tag="VGM46_12925"
     CDS             1367..2104
                     /locus_tag="VGM46_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEY4193542.1"
                     /translation="MSVNAIEGSGSSEFWQGVRLSLPVVIASAPFSMLFGALAVDNGF
                     SVFEAFLMSATVFGGASQMVGIELFGHQVAPWLIVLSIFAVNFRHVLYSAGIGRRIAH
                     WPPIQQAVGYFILTDPQFAIAEARAETGRQVGFVWYLGVGLPVYLFWVAETAIGAVIG
                     KMIPDTSALGIDFLLPIYFLGLVMGFRKRPLWLPVVIVSAAASILAYKTVGSPWHVTI
                     GALAGVLLAVLLPPAYSGVERRTEHEA"
     gene            2101..2397
                     /locus_tag="VGM46_12930"
     CDS             2101..2397
                     /locus_tag="VGM46_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006204878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD family protein"
                     /protein_id="HEY4193543.1"
                     /translation="MSTTLWITLFAAVATYLTRIGGHLVISRFKTIHPRVEAGLNAVP
                     AAVLTTLVAPSILTAGPAEWAALVVTGFASLRGGLMTMFLAGAAVLIICRQFVG"
     gene            complement(2394..>2662)
                     /locus_tag="VGM46_12935"
     CDS             complement(2394..>2662)
                     /locus_tag="VGM46_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008548048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M24 family metallopeptidase C-terminal
                     domain-containing protein"
                     /protein_id="HEY4193544.1"
                     /translation="VYGIRIENLILVTPAETIEGGDMPMHAFETLTLAPIDKRLIRTD
                     LLAREELQWLDAYHARVLAEIGPMVDGQTLAWLEKATAPLAHVA"
BASE COUNT          448 a          768 c          891 g          555 t
ORIGIN      
        1 ttgccgccat cgacgacagg cgtcggatcg ggttttcacg cttcctctat gcccttggca
       61 tccgccatat aggcgatacc aacgccaagc ggctggcgcg ccatttcctg tccttccagg
      121 ctttgcgaga ggcgggcgaa agcgcggtga tgccacaggg caagggcgac ccaggcaatg
      181 ccgcatggca ggaactgaac ggcatcaacg gcataggcgg cgtggttgcg gaggcgctgg
      241 tcgatttctt cggtgaagaa cacaaccgca ccgtggtcga cgccttgctg gccgaggtga
      301 cgccgctgga cgaagagccg gtcggcgggg tcgattcgcc agttgccggc aagacggtcg
      361 tgttcaccgg cgcgctcgaa aaaatgtcgc gcgacgaagc caaggcaatg gccgagaagc
      421 tgggcgccaa ggttgccgga tcggtgtcga agaagaccga cttggtggtg gcggggccgg
      481 gggccggctc caagctcaag cttgcttccg aactgggcat cgaagtgatt gacgaggatg
      541 cgtggttcac ccggatcggc agggttggtt gagcgtggat agtttcaaag gtttcggtga
      601 aaaggcgttt ccttttctca aggcgctcga ttttcaccaa agccgcgagt ggtttgtcga
      661 aaacaaggat ttgttcgaaa ccgaactgcg cgagcctctg ggcgatctgg tcgatacgct
      721 gacggtgcgg ttcgaggcgg cggggatcgg ccttaagggc gatcacaaga agtcgctgtt
      781 tcgcatcaac cgcgatgtcc ggttcgccaa ggacaagcgg ccctataacc ggcatgtttc
      841 agcaatcctt tcgcccgacg gcagcaagat ggagcagggc gtgttcttcg tgcatatcgg
      901 gctggagcgc tgctttgctg ccatcgcctg gtggggcgtg gcgcctgaac tgttgttggc
      961 catgcgaagg gcgattgcgg accggccggg agattttcgc gccatgaccg ccgcgttgaa
     1021 gcgcaacggt ctggagcttg gcagcgaagg ggcgatgaag cgaatgccgc gcggtttcga
     1081 gcaggtaggc gacgccgatc ttgcggcggc gatccgcaac cgccatttcg tcgtgcgcca
     1141 ggaaatcgat ccggcaggca tccatacagc cgcgctggcg gatgaacttt acggtttcgt
     1201 tcagcgtgcg cgcccgctgc tcgaatgggg cagggccatc gaaggcaaga cggcggcaca
     1261 ataaatcaac gcccaacgat cacttcggct gatggctgca ctcaggcaaa ttggtgtgac
     1321 aagccggatg atgaaccgta gatttgcggc tcccaaggaa cagttcatgt cggtgaatgc
     1381 gatcgaaggc tcgggatcga gcgaattctg gcaaggcgtg cgcctgagcc tgcccgttgt
     1441 cattgcatca gcgccgtttt caatgctgtt cggcgcattg gccgtcgaca atggtttttc
     1501 ggtgttcgag gcgttcttga tgagcgccac ggtgtttggc ggcgcgtcgc agatggtcgg
     1561 catcgaactg ttcgggcacc aggtcgcgcc atggctgatc gttttgtcga tctttgccgt
     1621 gaattttcgc cacgttctct attcggccgg cattggccgc cgcatcgcgc actggccgcc
     1681 catccagcag gcggtcggat acttcatcct gacggacccg caatttgcca ttgccgaggc
     1741 gcgggcggaa accggcaggc aagtcggctt cgtctggtat ctcggcgtcg gcctgccggt
     1801 ttacctgttc tgggtcgctg aaacggcgat cggagcggta atcggcaaga tgatccccga
     1861 taccagcgcg ctcggcatcg acttcctgct gccgatctat ttcctcggcc tcgtcatggg
     1921 ctttcgcaag cggccgctct ggctgcccgt ggttatcgtc agcgccgccg cttccatcct
     1981 tgcctacaag acggtcggct cgccatggca cgtcaccatc ggtgcgctgg ccggcgtgtt
     2041 gctggcggtg ctgctgccgc cggcctatag cggcgtcgaa cgccggacgg agcatgaggc
     2101 atgagcacga cgctgtggat cacgctcttt gcggctgtcg ccacctatct gacccgcatt
     2161 ggcgggcatc tggtgatttc gcggttcaag accattcatc cgcgcgtcga ggccgggttg
     2221 aacgcggtgc cggcggcggt gctgaccacg ttggtcgcgc cctccatcct gacggctggt
     2281 cctgccgaat gggcggcatt ggtcgtcacc gggttcgcct cgttgcgtgg cgggctgatg
     2341 acgatgttcc tggcgggggc cgcggtgctg attatctgcc ggcagttcgt cggttaggcg
     2401 acgtgcgcca gcggcgctgt cgccttttcc agccaggcga gcgtttggcc atcgaccatc
     2461 ggtccgatct cggccagcac gcgggcgtgg taggcatcca gccattgcaa ttcttcccgc
     2521 gcgagcagat cggtacggat gagccgcttg tcgatgggcg ccagcgtcag cgtctcgaag
     2581 gcgtgcatcg gcatatcgcc gccctcgatt gtctctgctg gtgtgaccag gatcagattc
     2641 tcgatgcgga tgccgtagac gc
//
LOCUS       DASYCS010000422         2442 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3171052,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000422 DASYCS010000000
VERSION     DASYCS010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3171052"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="VGM46_12940"
     CDS             complement(<1..483)
                     /locus_tag="VGM46_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY4193545.1"
                     /translation="MTVLLAGAAAVALLATSALAQETAPAKAGETANEQAKPAQGETL
                     LDKILVISRTGETAIESLSSSSHVDKEQLDRRMATTPNDVFFGVPGITMQSDAKRVAT
                     SVNIRGLQDFGRVQVIVDGARQDFSRSGHGTGSTFWIDPDLIQSADVIRGPVANTYGS
                     G"
     gene            806..1015
                     /gene="hemP"
                     /locus_tag="VGM46_12945"
     CDS             806..1015
                     /gene="hemP"
                     /locus_tag="VGM46_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006335375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HEY4193546.1"
                     /translation="MNAYNPDAFRTRFHRPETEGKRRPEPNRISVRTLSSNTLFAGEH
                     EIGIEHHGALYRLKITRLGKLILNK"
     gene            1025..2083
                     /locus_tag="VGM46_12950"
     CDS             1025..2083
                     /locus_tag="VGM46_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009756008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin-degrading factor"
                     /protein_id="HEY4193547.1"
                     /translation="MDQRVKPAPHEIRNARSDNPKMRERDLAAQLGISEAELVAAHCG
                     DGVVRLDPRVNDLLTGLEAVGEVMALTRNESAVHEKIGIYDKVVTGTHNAMALGKDID
                     LRIFPGVWTHGFAVEKRDGDDIRRSLQFFDAAGDAVHKVHLRPASNLYSYQKLVAALE
                     SANQDATVDIAPRADSDPEMSDALVDVTELRERWSKLTDIHQFFGMLRALKVGRRQAI
                     RLVGQDYAWKIDNGAVGSMLHHAAADALPIMCFVGNRGCIQIHSGPIANIKPMGPWVN
                     VMDETFHLHLRVDHIHEAWAVRKPTKDGHVTSIEAYGEDGELIIQFFGQRHEGEQERP
                     DWRFLAENLPRIPVSGVV"
     gene            2094..>2442
                     /locus_tag="VGM46_12955"
     CDS             2094..>2442
                     /locus_tag="VGM46_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY4193548.1"
                     /translation="MAGLLKLARSALIALSFTLAPVGAGASENTSVFPAGAKIASIGG
                     SITEIVFALGEQDRLVARDSTSVYPQEAFKLPDVGYMRQLSPEGVLSVDPSGILALQG
                     SGPKEAIDVLKKAS"
BASE COUNT          467 a          737 c          771 g          467 t
ORIGIN      
        1 gccggaaccg taggtgttgg ccaccgggcc gcgaatgacg tcggcggact ggatgagatc
       61 gggatcgatc cagaacgtgg acccggtgcc gtggcccgaa cggctgaagt cctgccttgc
      121 gccatcgacg atcacctgaa cgcggccgaa atcctgaagg ccgcgaatgt tgacgctggt
      181 ggcgacgcgc ttcgcgtcgg attgcatggt gatgcccggc acgccgaaga agacgtcgtt
      241 gggcgtcgtc gccatgcggc ggtcgagttg ttccttgtcg acatggctgg aggacgaaag
      301 cgattcaatg gccgtctcgc cggtgcggct gatcaccagg atcttgtcca gcagcgtctc
      361 gccctgcgcg ggcttggcct gttcgttcgc cgtttcaccg gccttggccg gggcggtctc
      421 ctgcgccagg gccgaggtcg ccagaagcgc caccgcggcc gcgccggcaa gcaataccgt
      481 cattccgttt aagagcggcc cgtttaagag cggcccattg atgagcggcc gatttttacc
      541 cgcatgccgc aatagcgggc gcgcgccatc cctcaactct gccccggccc aattcaaccc
      601 catgttccca ctccaaaatc caagttaatg ctggctggct tggggctgac ccggcgcttg
      661 ctgcaatttt tgcgctgccg gtatcttaaa tgttgactca tttactccgg tattgcactg
      721 actattaaac gtgagtattc gagtcaagaa tgattttgaa cggcgcaggc tgcggcagca
      781 gcgcggcgcg agaaaggagc cgaagatgaa cgcttacaat cccgacgctt tccgtacccg
      841 cttccaccgc cccgagaccg agggcaaacg ccggccggaa cccaaccgca tttcggttcg
      901 gacgctgtcc agcaacacgc tgtttgccgg cgagcacgag atcggcatcg aacaccatgg
      961 cgcgctgtat cgattgaaga tcacccgcct gggcaagcta attctcaaca agtaggtcga
     1021 agtcatggat cagcgcgtaa agcccgcccc gcacgaaatt cgaaacgcac ggtcggacaa
     1081 cccgaagatg cgcgagcgcg accttgccgc gcagcttggg atttccgaag ccgaactcgt
     1141 tgcggcgcat tgcggcgacg gcgtcgtgcg cctcgacccg cgcgtaaacg acctgctcac
     1201 cggccttgag gcggtgggcg aggtgatggc gctgacccgc aacgaaagtg cggtccacga
     1261 aaagatcggc atctacgaca aggtggtgac cggaacccat aatgcgatgg cgctgggcaa
     1321 ggatatcgac ctgcgcatct tcccaggcgt gtggacgcat ggctttgccg ttgaaaagcg
     1381 cgacggggac gacatccgcc gcagcctgca atttttcgat gccgccggcg atgcggtcca
     1441 caaggtgcat ctgcggccgg catcgaacct gtattcctac cagaaactgg tcgccgcgct
     1501 ggaatcggcc aatcaagatg cgacggtgga tatcgcgccc cgtgccgaca gtgatcccga
     1561 aatgtccgat gcgttggttg acgtcacgga gctgcgtgag cgctggagca aactgaccga
     1621 tatccatcag tttttcggca tgctgcgcgc gctgaaagtc ggccgccggc aagcgattcg
     1681 ccttgtcggg caggattatg cgtggaagat cgacaatggc gctgtcggct cgatgcttca
     1741 ccatgcagcc gcagatgcgc tgccgatcat gtgctttgtc ggcaaccgcg gctgcatcca
     1801 gatccattcg gggccgatcg ccaacatcaa gccgatgggg ccgtgggtca acgtgatgga
     1861 cgagacgttc cacctgcatc tgcgcgtcga tcatattcac gaggcctggg cggtgcgcaa
     1921 gccgacaaag gacggccatg tcacttccat agaggcttat ggcgaggatg gggaactgat
     1981 tatccagttt ttcggccagc ggcacgaagg cgagcaggaa cggccggatt ggcgcttcct
     2041 ggccgagaac ctgccccgaa ttcccgtttc cggcgtggtt tgaggggtcg tcaatggcag
     2101 gtttgttgaa actggcgcgg tcggcgctga tcgctctttc gttcacgctg gcgcccgtag
     2161 gcgccggtgc gtcggaaaac acatcggtct ttccagccgg cgcgaagatc gcctcgatcg
     2221 gtggctcgat caccgaaatc gtgttcgccc tgggcgagca ggaccggctg gtcgcccgcg
     2281 attcgaccag cgtctatccg caagaggcgt tcaagctgcc ggatgtcggc tatatgcgcc
     2341 agctttcgcc ggagggcgtg ctttcggtcg atccttccgg cattctggcc ttgcagggaa
     2401 gcggcccgaa ggaagccatc gacgtgctca agaaggccag cg
//
LOCUS       DASYCS010000423         6350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4475691,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000423 DASYCS010000000
VERSION     DASYCS010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 6350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6350
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4475691"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="VGM46_12960"
     CDS             complement(<1..881)
                     /locus_tag="VGM46_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEY4193549.1"
                     /translation="MALASTIQRDWFSNVRADLLSGIVVALALIPEAIGFSVIAGVDP
                     KVGLYASVVIASVIAFVGGRPAMISAATAATAVLMGPLVREHGVQYLFAATILMGIVQ
                     IVAGLLKLGRLMRFVSQSVMTGFVNSLAILIFLAQMPQLVHVPMATYGLIALGLAIIY
                     IFPRFTKAIPSPLVAIAVLTIVSLVFKLDVRAVADLGELPTTLPSFGLPEVPLTFETL
                     RILLPVALTLAAVGLLESLLTAQIVDDMTDTPSDKSRECAGQGVANITSAFFGGMGGC
                     AMIGQSVINVSSGGRGRL"
     misc_feature    complement(951..1006)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03058"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="sul1 is cis-regulatory element that is thought to
                     sense ions involved in sulfur or methionine metabolism;
                     They are found in Alphaproteobacteria; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03058"
     gene            complement(1165..1719)
                     /locus_tag="VGM46_12965"
     CDS             complement(1165..1719)
                     /locus_tag="VGM46_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY4193550.1"
                     /translation="MRKLRADSIRNRERLLEAATHVFSTGGSQASLEMVARQAGVGIG
                     TLYRHFPTREALFEAVYRHEVDHLGGLAEQLAGVGDPVEALRTWLHANVRLVAAKKGM
                     IEALQMVAHGSSELKAYSLERLTNAIALLLDHGIAAGRLRADVTPEELLRTLLGIFYS
                     QATNDWQPSALRLVDVFVDGLRKR"
     gene            1942..2454
                     /locus_tag="VGM46_12970"
     CDS             1942..2454
                     /locus_tag="VGM46_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012454806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY4193551.1"
                     /translation="MSPSISLSINGQPCGVAVDDPRVTLLDLLREKLGLTGTKKGCDR
                     GQCGACTVLLDGRRINSCLVLAASLDGAEVVTIEGLADGDTLHPVQAAFIAHDGFQCG
                     FCTPGQIMSAVGLIREAHAGNEPERIRELMSGNICRCGAYQGITEAVLEAQKTLADKE
                     GSKQDRKDAA"
     gene            2451..3509
                     /locus_tag="VGM46_12975"
     CDS             2451..3509
                     /locus_tag="VGM46_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012030137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEY4193552.1"
                     /translation="MNRFDYIRPASVQEAVAAAAMPGSTYLAAGTNLLDLMKVGVSRP
                     ERVIDISRLPGLDRIEWQGDGGVRIGAMVRNSDLAYDQRFARSFPAIAEALLSGASAQ
                     LRNAATVSGNLMQRTRCAYFQDTASACNRRAPGSGCDAREGENRTMAVLGFSEHCIAT
                     HPSDFCVPLVAYDAVVEVEGPHGAREIAVDALHALPGTTPEQETVLMPGEMIVALRLP
                     AEAADFARNARYLKLRERTSFAFALVSAAVALDIDNGVIRNARIALGGVAAKPWRARE
                     AEASLKGSPPTDEAFEKAAELALAEAKPSGDNAFKIELARRLTRRALAFALEGTPNVM
                     PALPASPFMTNPGASHVA"
     gene            3499..5754
                     /locus_tag="VGM46_12980"
     CDS             3499..5754
                     /locus_tag="VGM46_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY4193553.1"
                     /translation="MSLETTPSQNRYGSNIGQPLTRRDGVLKVTGRATYAADNHPAGM
                     LYAVTAVSRIARGRVTSLNVAAAMAHPGVVEVITPANRPPLAHDPDEKMPPFGFRVEV
                     LQDDTVRYANQPIALVIAETFEAATEGAALLDPRYEAEVPRTGRGNGLVYDPPAIGIG
                     APPRTARGGDIEEGLAAAVNVTEEEYLTPAQYHNAMEPHAIVAEWDGDKVTLDMPNQA
                     MVLSRASYGAYFGVPPENVLIRSPFLGGGFGSKAILNGPQILAILAARMLKRPVKLSL
                     TRAQMFGPVGHRGATWQKLRIGTDRQGHITALHHHSLSATSSFDDFLESAANASLPLY
                     ASPALLADHSGLRLNTGTPGPMRAPGEASGSAALEVAMDEAAEACGMDPLEFRLANYA
                     ETEPGTGKPFSSKALRECYAEGAKRFGWSGRPLAPRQMRDEKGFLVGWGMGTALFHCP
                     HFPASARATLRADGSALVETAGADMGQGAWTALAQIAAEALGLDPEQVELHSGLSTLP
                     DGGVAGGSAHTASAGLALHNAGQDAILKLAELAMSDAGSPLFGAGNIGVTARGGRLYR
                     NDDQTRSESYAEILARAGRSEVIGAADASRDPAAAGQHAMFSHGAVFAEVKVDPDLGQ
                     VRVTRLVGAFAAGRIINPRLVRSQYFGGMIWGASFALHEEAIVDRRTGRIMNSDLAEY
                     RVPVNADIPSLEAILIPEEDPYVNPLGIKGVGEIGITGTVGAVANAVWHATGKRVRRF
                     PIRIEDLLTDR"
BASE COUNT         1136 a         2063 c         2102 g         1049 t
ORIGIN      
        1 agccttccgc gcccgccgga cgaaacattg atgaccgatt gcccgatcat cgcacagccg
       61 cccatgccgc caaaaaaggc ggaggtgatg ttggcgacgc cctggccggc gcattcgcgg
      121 ctcttgtcgc tgggcgtatc cgtcatgtcg tcgacgatct gcgccgtgag cagcgattcc
      181 agcaggccaa cggcggccag tgtcagcgcc accggcagca ggatgcgcag cgtctcaaag
      241 gtaagcggca cttcgggcag tccaaagctc ggcagggtgg tcggcaattc gccgagatcg
      301 gcgacggcgc gcacgtcgag cttgaaaacg aggctgacaa tggtcagcac cgcgatcgcc
      361 accagcggcg aagggatcgc cttggtgaag cgcgggaaga tgtagatgat cgcaaggccg
      421 agcgcgatca gcccgtatgt cgccatcggc acatggacga gttggggcat ctgcgccaga
      481 aaaatcagga tcgccagcga attgacgaag ccggtcatga ccgattgcga gacgaagcgc
      541 atcaaccgtc caagcttcag caggccggcg acgatctgga cgatgcccat caggatggtg
      601 gcggcgaaga gatattgcac gccatgttcg cgcaccagcg gccccatgag gacggcggtt
      661 gccgccgtcg ccgccgagat catcgccggc ctgccgccga cgaaggcgat gacggaggcg
      721 atgaccaccg aggcataaag cccgaccttg gggtcgacgc cggcgatgac cgaaaacccg
      781 atggcctccg gtatcaatgc cagcgcaacg acgatgcccg agagcaggtc ggcgcggaca
      841 ttggaaaacc agtcgcgttg aatggtggaa gccagagcca tggtgattaa atcccgcaag
      901 gacgcccagg ccggcgcatg tcgcgcggct gacggtggtc atttcatttg cgtggtccgg
      961 cggattggcg gccggaagag ccacccggat tttcaccggg tccagtcgaa tgagcagcct
     1021 tataatggag attctttgca acgcaatatc gaagggcggc agatcgcaaa tcggcctgga
     1081 gattggcgtc agccatggga gtggatgaag caccggacgc gccggtgctt gacgcgtcaa
     1141 tccttgcggc gtcggcgctg gccatcaccg cttgcgcagg ccgtccacga aaacatcgac
     1201 gagccgcagc gcgctcggtt gccagtcatt ggtcgcctgc gaatagaaga tgccgagcag
     1261 cgtgcgcagc agctcttcgg gcgtgacgtc ggcgcgcagc ctgccggcgg cgatcccgtg
     1321 gtccagcagc agggcgatgg cgttggtcag ccgctccagc gaataggctt tcagctcgct
     1381 cgacccgtgc gcaaccattt gcagcgcttc gatcatgccc ttcttggcgg cgaccaggcg
     1441 gacattggcg tgcagccacg tccgcagcgc ctcgaccgga tcgcccacgc cggccagttg
     1501 ttcggcgagg ccgccgagat ggtccacctc atgccggtag acggcctcga acagcgcctc
     1561 gcgcgtcggg aagtggcggt agagcgtgcc gatgccgacg ccggcctggc gcgccaccat
     1621 ctccaggctc gcctgggagc cgccggtgct gaagacatgg gttgccgctt ccagcagccg
     1681 ttcacggttg cggatcgaat cggcgcgcaa tttgcgcatt gtcttggtgg cttcctgttc
     1741 catttctctc aggataacac agtttgtctc ttgataaacg gaggcatcct ccgtatagat
     1801 aggtggtgcg tcgggaaaac gaacagagct atacgcattc ccgaccaaag ccggcggatt
     1861 ggccgcttcc gtttgcgctc gcattttccg ccgcctccgg caacccgaac cctacatcat
     1921 aaacactgcg tggagtatta aatgtctccc tccatcagcc ttagcatcaa tggccagcct
     1981 tgcggcgtcg cggtggacga tccgcgcgtg accctgcttg acctgctgcg cgaaaagctc
     2041 ggcctgaccg gcaccaagaa aggctgcgac cgtggccagt gcggcgcctg caccgtgctc
     2101 ctcgatggcc ggcgcatcaa ttcgtgcctg gtgctggccg ccagcctgga tggcgcggaa
     2161 gtcgtcacca tcgaaggcct tgccgatggc gatacgctgc atccggtcca ggcggcgttt
     2221 atcgcccatg acggatttca atgcggcttc tgcacgccgg ggcagatcat gagcgccgtc
     2281 gggcttatcc gcgaggccca tgccggaaac gagccggagc gcattcgcga gctgatgagc
     2341 ggcaatatct gccggtgcgg cgcctatcag ggcatcacgg aagccgtgct ggaagcccag
     2401 aaaacgttgg ctgacaagga aggctccaag caggaccgaa aggacgcggc atgaaccgtt
     2461 ttgactatat ccgccccgcc agcgtccagg aggcggttgc ggcggcggcc atgccaggtt
     2521 ccacctatct ggcggccggc accaacctgc tcgacctgat gaaggtgggg gtctcgcggc
     2581 cggaacgggt gatcgacatt tcccggctgc ccggcctcga ccggatcgaa tggcaaggcg
     2641 atggcggcgt gcgcatcggc gcgatggttc gcaattccga cctcgcctat gaccagcgct
     2701 tcgcccggtc ctttcccgct attgccgaag cgttgctttc gggcgcgtcg gcgcagttgc
     2761 gcaatgccgc gacggtcagc ggcaacctga tgcagcgcac gcgctgcgcc tatttccagg
     2821 acaccgccag cgcctgtaac cggcgcgcgc cggggtccgg ctgcgatgcg agggagggcg
     2881 agaaccgcac gatggcggtg ctgggcttca gcgagcattg catcgccacc catccatcgg
     2941 atttctgcgt gccgctggtc gcctatgacg cggtggtcga ggtcgaaggc ccgcatggcg
     3001 cgcgcgaaat cgcggtcgac gcgctgcatg cgctgcccgg caccacgccg gaacaggaga
     3061 cggtgctcat gccgggcgag atgatcgtcg cgctgcggct gccggccgag gccgcggatt
     3121 tcgcgcgcaa cgcccgctac ctgaagctgc gcgagcggac atccttcgcc tttgcgctgg
     3181 tttcggcggc agtcgccctc gatatcgaca atggcgttat ccgcaacgcc cgcatcgcac
     3241 tgggcggcgt cgccgccaag ccatggcggg caagggaggc cgaggcctcg ctgaaggggt
     3301 cgccgccgac agacgaagct ttcgaaaaag ccgccgaact ggcgctggcc gaagcaaagc
     3361 cttccggtga caacgccttc aagatcgaac tggcccggcg cctgacgcgg cgcgcgcttg
     3421 cctttgccct ggagggaacg ccgaacgtga tgcctgcctt gccggcttcg cctttcatga
     3481 ccaatcccgg agcaagccat gtcgcttgaa acaacaccgt cccagaaccg gtatggatcg
     3541 aatatcggcc agccgctgac acgccgcgac ggcgtgctga aggtaaccgg ccgcgccacc
     3601 tatgccgccg acaaccaccc ggcgggcatg ctctatgccg tcaccgccgt cagccgcatc
     3661 gcgcgcggcc gcgtgacgtc gctgaacgtc gccgccgcca tggcgcatcc gggcgtcgtc
     3721 gaggtcatta ccccggccaa ccgtccgccg ctggcgcacg accccgacga aaagatgccg
     3781 cctttcggct tccgcgtcga agtgctgcaa gacgatacgg tacgctatgc caaccagcct
     3841 atcgcgctgg tgatcgccga gacgttcgag gcggcgaccg agggggcagc cctgctcgat
     3901 ccgcgctatg aggccgaggt tcctcgcacc ggccgtggca atggcctggt ctacgatccc
     3961 ccagcgatcg gcatcggcgc gccgccccgc accgcgcgcg gcggcgatat cgaggaaggc
     4021 ctggcagctg cggtcaacgt cacggaagaa gaatatctga cgccggcgca atatcacaat
     4081 gcgatggaac cgcacgccat cgtcgccgaa tgggatggcg acaaggtcac gctcgacatg
     4141 cccaaccagg cgatggtgct cagccgcgcg tcctacggcg cctatttcgg cgttccgcct
     4201 gaaaatgttc tcatccgctc gcccttcctc ggcggaggct tcggctccaa ggccatcctg
     4261 aacggaccgc aaatcctggc gatcctggcg gcgcggatgt tgaagcggcc ggtcaagctt
     4321 tcgctgacgc gcgcgcagat gttcggaccg gtcgggcatc gcggggcgac gtggcagaag
     4381 ctgcgcatcg gcaccgacag gcaaggccac atcacggccc tgcatcatca ttcgctgtcg
     4441 gccacttcga gcttcgacga tttcctcgaa tcggcggcga acgcatcgct gccgctctat
     4501 gccagcccgg ccctgctggc cgaccatagc ggcttgcgcc tgaacacggg aacgcccgga
     4561 ccgatgcgcg cgcccggcga agcctccggt tcggcggcgc tcgaagtcgc catggacgag
     4621 gcagccgaag cctgcggcat ggacccgctg gaattccggc ttgcgaatta tgcggagacg
     4681 gagccgggca ccggcaagcc gttctcgtcg aaggcgctgc gcgaatgcta cgccgagggc
     4741 gccaagcgtt tcggctggtc ggggcggccg ttggctccgc gccagatgcg cgacgaaaaa
     4801 ggcttcctgg tcggatgggg catgggcacg gcgctgtttc attgcccgca tttcccggcg
     4861 tcggcgcggg cgaccttgcg cgccgacggc agcgcgctgg tggagacggc cggcgccgac
     4921 atggggcagg gcgcatggac cgcgcttgcc cagatcgcgg ccgaggcgct cggtctcgat
     4981 cccgagcagg tcgagctgca ttccggcctg tcgaccttgc ccgatggcgg cgtcgccgga
     5041 ggttccgccc atacggcaag tgccggcctg gcgctgcaca atgccgggca ggatgcgatc
     5101 ttgaaactgg ccgaactcgc catgtcggat gccggctcgc cgcttttcgg cgcaggcaat
     5161 atcggcgtta cggcgcgtgg cgggagactt taccgcaacg acgatcaaac gcgcagcgaa
     5221 agctatgccg agatacttgc ccgcgcgggc cgcagcgaag tcatcggcgc cgccgacgcc
     5281 tcgcgcgatc cagcggcggc agggcagcat gccatgttct cgcatggcgc cgtcttcgcc
     5341 gaggtcaagg tcgatcccga tcttggccag gtccgcgtca cgcggctggt cggcgcgttc
     5401 gcggccggcc ggatcatcaa tccgcgcctg gtgcgcagcc agtatttcgg cggcatgatc
     5461 tggggcgcgt cgttcgcgct gcatgaagaa gccatcgtcg accgccgcac cggcaggatc
     5521 atgaactcgg atctggccga ataccgggtg ccggtgaatg ccgacattcc ctcgctggaa
     5581 gcaatcctca ttcccgagga agacccctat gtcaatccgc tcggcatcaa gggcgtcggt
     5641 gagatcggca tcaccggaac ggtcggcgcg gtcgccaatg ccgtctggca tgcgacgggc
     5701 aagcgggtgc gccgcttccc gatccgcatc gaggaccttt tgacggacag gtagacgagg
     5761 ccctccaacg gtccgccgct tggcactacc ggccgcagat agaggccgta taacggtcca
     5821 ccgccgccgg cctcggatcg aggccgttcc ggccaagtgc cacgcgctgc cggccgcaaa
     5881 tgtggtggcg ggctgatgta ggaagcctcg gcatcacgcc cggccccgat ccttgcggca
     5941 tcgagccgga catgatggcc gtcacaaggg ggccggcctc gcgcttgtgc gctgccgagc
     6001 gcgatcttcc atcgaagctc cgggctcgcc gggtcggttc ttcggaagcg gagggcggcg
     6061 ggcaggcgtt aatttcgagc gggacttgaa attccggcca gtgtgagctt accaacgagg
     6121 gcggcgcggc gcgtcggcgc gaaccgatgg tggagaacag gtatatttgc cgcaaatgtc
     6181 atggaaagtt ccgacaagcc ggtttgcgcg acatgacgtg cccgccatga aacggcaaac
     6241 gaccctgctc gatgcattta cgggcagctg gcgcgtgcga cgcaagatca tcgatcaccg
     6301 cgtggccagc gtttcgaatt tcgtcggcga ggccgtgctg acgaagactt
//
LOCUS       DASYCS010000424         2935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3171216,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000424 DASYCS010000000
VERSION     DASYCS010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3171216"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VGM46_12985"
     CDS             <1..336
                     /locus_tag="VGM46_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4193554.1"
                     /translation="LSIARAIITRPKLIVADEPVSALDVSIRAQILDLFAELNGKLGI
                     AYLFITHDLNVARAITDEVLVMQDGRIVERGKTGEVLTRPQSDEAQALVAAAPDLRRA
                     LARKMHEQG"
     gene            complement(413..844)
                     /locus_tag="VGM46_12990"
     CDS             complement(413..844)
                     /locus_tag="VGM46_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015317458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HEY4193555.1"
                     /translation="MFIGLLDQLRELLDGDPGVRKVADDPVLTAELLMLFRMILADGT
                     VSESEMTAFRRICRDAFEIPESSIERVIEYLNDFGYETDGSQAVALFRDLDMERRKLL
                     ALHMAEIAKADSHLAESEMKLLRRTLDLLGISSGDATGSSL"
     gene            961..1746
                     /locus_tag="VGM46_12995"
     CDS             961..1746
                     /locus_tag="VGM46_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HEY4193556.1"
                     /translation="MSDLSAQVILMLALAGFVAGFVDSIAGGGGLITVPALLLAGLPP
                     VASLGTNKLQGLFGSGSATLAYASKGHVDLRRQLPSALMAAGGGALGALAATVLPGDI
                     LSAALPVILVAIALYFAIKPNMGDVDRVRRLSPFLFGLLVVPAIGFYDGVFGPGAGSF
                     YMLAFVSLAGYGVLKATANTKLLNFASNLGGFIIFASVGVISWKIGLMMGVAQFLGAR
                     TGASLAIQSGAKIIKPLLVIVCVALAIKLLADPQNPLRLMIGL"
     gene            complement(1806..2462)
                     /gene="gmk"
                     /locus_tag="VGM46_13000"
     CDS             complement(1806..2462)
                     /gene="gmk"
                     /locus_tag="VGM46_13000"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEY4193557.1"
                     /translation="MVAKDVGSRIRRRGLMLVLSSPSGAGKSTIARNLIESDQGFELS
                     VSVTTRPRRGSEIDGIHYHFRTQREFEILRDNHELLEWAEVHGNFYATPREAAEKAMA
                     EGRDMLFDIDWQGAMQLKEKMRGDIVSIFILPPSMKELKARLKRRAEDQEAAIETRLK
                     NARTEIEHWTEYDFVVLNDDLDRAFSEVRAIVSAERLRRDRRPGLFDFVSNLLDEKTE
                     "
     gene            complement(2466..>2935)
                     /locus_tag="VGM46_13005"
     CDS             complement(2466..>2935)
                     /locus_tag="VGM46_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1732 domain-containing protein"
                     /protein_id="HEY4193558.1"
                     /translation="QALSGLVAARQAEGEALGVLLSGQIDAIEALTLRAEADPSREPA
                     AIRAKLTEQVRLLLDASANLDEGRLHMEAAFLATKADIREEIDRLKTHVASARGLLAA
                     GGAIGRKLDFLAQEFNRESNTLCSKSNAATVTAIGLELKAGVDQFREQVQNLE"
BASE COUNT          511 a          978 c          832 g          614 t
ORIGIN      
        1 ctgtcgattg cccgcgccat cataacccgg ccgaagctga tcgtcgccga cgaacccgtc
       61 tcggcgctcg atgtctccat tcgcgcccaa atcctcgacc tgttcgccga actgaacggc
      121 aagcttggca tcgcctatct attcattacc cacgacctca acgtggcgcg cgcgatcacc
      181 gacgaggtgc tggtgatgca ggacggcagg atcgtcgaac gcggcaagac cggcgaggtg
      241 ctgacccgcc cgcaatccga tgaggcgcaa gcgctggtgg cggccgcacc ggacctgcgc
      301 cgggcgctgg ccaggaaaat gcacgaacag ggctaggccg cctgccttgc cggttcgatc
      361 attatgctcg acgcatcgtg gctcgcggca gcggcgtgct ccaacgatcc actcaaagcg
      421 acgaaccagt cgcatcgccg gagctgatgc cgagcaggtc tagcgtacga cgcagcaact
      481 tcatctcgct ctccgccagg tggctgtccg ccttggcgat ctcggccatg tgcagagcca
      541 gcaatttgcg ccgttccata tcgaggtcgc gaaacagcgc caccgcctgc gaaccatccg
      601 tctcgtaacc gaaatcgttg agatattcga taacgcgctc gatgctgctt tcgggaattt
      661 caaaggcatc gcggcaaatg cgccgaaatg ccgtcatctc gctttcgctc accgtgccgt
      721 ccgccaggat cattcgaaac agcatcagca gttcggcggt gagaaccgga tcgtccgcta
      781 ccttgcgaac gccgggatcg ccgtcgagaa gctcgcgcaa ctggtccagc aagcctatga
      841 acatgaagcc ttctcccgct cgtcacgcgt attttgagga cgagaccgcc attgcgcaat
      901 ccccgccgat gtggtcgaac ccgcatctac cttatcgccc gcccggatac ccgtcccgcc
      961 atgtccgatc tttccgcaca agtcatcctc atgcttgcgc tggccggctt tgtcgccggc
     1021 ttcgtcgatt ccatcgccgg cggcggcggg ctgattaccg tgccggcgct gctgttggcc
     1081 ggcttgccgc ctgtcgcgtc cctcggcacc aataaattgc agggcttgtt tggttccggc
     1141 tcggccacgc tcgcctacgc ctcgaaaggc catgtcgatc tgcgccggca actcccttcc
     1201 gccttgatgg cggcaggcgg tggagcattg ggggcactgg cggcgaccgt cctgcccggc
     1261 gacatcttga gcgcggcact tcccgtcatt ctggtggcca tcgcgctcta tttcgccatc
     1321 aagcccaata tgggcgatgt cgaccgcgtt cggcgcctct ctcccttcct gttcggcctg
     1381 ctggtcgtgc ccgccatcgg cttttacgat ggcgttttcg gtccgggggc cggatcgttc
     1441 tatatgctgg ctttcgtttc gctcgccggt tacggcgtac tcaaggcgac ggccaatacc
     1501 aaactgttga atttcgcctc caacctcggc ggcttcatca tcttcgcttc ggtcggcgtc
     1561 atcagctgga agatcggcct gatgatgggc gtggcgcaat ttctcggcgc gcgaaccggc
     1621 gcttccctgg cgatccaaag cggcgccaag atcatcaagc cgctgctggt gatcgtgtgc
     1681 gtggcgctgg ccataaagct gttggccgac ccacaaaacc cgcttcgcct gatgatcggc
     1741 ctttgaccgc tgccccccaa tggtcggccc gaaatggcca gtccaaaatg accggtattt
     1801 gaaaattatt cggtcttttc gtccagcagg ttggaaacga aatcgaaaag gccggggcgc
     1861 cggtcacgcc gcagacgttc ggcgctgaca atggcgcgca cttcggaaaa tgcccggtcg
     1921 aggtcgtcat tgagcaccac gaaatcatat tcggtccagt gctcgatttc ggtgcgcgcg
     1981 tttttcagcc gcgtctcgat ggccgcttcc tgatcctcgg cgcgccgctt cagccgcgcc
     2041 ttcagctctt tcatcgacgg cggcaggatg aagatcgaga cgatatcgcc gcgcatcttc
     2101 tctttcagct gcatcgcccc ctgccagtcg atatcgaaca gcatgtcgcg cccctcggcc
     2161 atcgcctttt cggctgcctc gcgcggcgtt gcatagaaat tgccatgcac ttcggcccat
     2221 tcgagcagtt cgtgattgtc gcgcaatatc tcgaattcgc gctgcgtcct gaaatgatag
     2281 tggatgccgt cgatttcgct gccgcggcgt gggcgcgtcg tcacgctcac cgacagttcg
     2341 aacccctggt cgctttcgat caggtttcgc gcgatggtcg acttgccggc ccccgaaggt
     2401 gacgacaaaa caagcatcag gccccggcga cgaatgcgcg aacccacgtc tttggcaacc
     2461 atcggttact ccagattctg gacttgttcg cgaaactgat cgacccccgc cttcagttcc
     2521 agcccgatgg ccgtcaccgt cgcggcattc gatttcgaac acagcgtatt cgattcgcga
     2581 ttgaattcct gcgccagaaa gtcgagcttt cgtccgatgg cgccgccggc ggccagaagc
     2641 ccgcgtgccg acgcgacatg cgttttcagc cgatcgattt cttcgcggat atcggccttg
     2701 gtggcaagga aagccgcctc catatgcagc cgtccctcgt ccagattggc cgaggcgtcc
     2761 agaagcaacc gcacctgttc ggtaagtttc gcacggattg ccgccggttc cctcgacgga
     2821 tcggcctcag cgcgcagcgt caacgcctca atggcgtcga tctgcccgga tagcagcact
     2881 cccagcgcct cgccttcggc ctggcgggca gccaccaggc cagacaatgc ctgtt
//
LOCUS       DASYCS010000425         1678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4765626,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000425 DASYCS010000000
VERSION     DASYCS010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4765626"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="VGM46_13010"
     CDS             complement(<1..900)
                     /locus_tag="VGM46_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009338484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY4193559.1"
                     /translation="MNIRNIAIIAHVDHGKTTLVDQLLKQSGSVRENQHMAERAMDSN
                     DQERERGITILAKCTSVEWHGTRINTVDTPGHADFGGEVERILSMVDGVVLLVDAAES
                     VMPQTKFVLSKALKLGLKPIVVINKIDRQDAQPKETLESIFDLFLALEANDDQLNFHY
                     LYASGRNGWAVKELEDERKNLDPLFQLIVDNVPAPKPQALAGEEHPFKMLVTTLEADN
                     FLGRILTGRIESGVITTNRNIKALNRDGEIIEKTRVTKLLAFRGLARVPVERAEAGDI
                     VAIAGLDDATVADTLCDLNVDMPI"
     gene            1331..>1678
                     /locus_tag="VGM46_13015"
     CDS             1331..>1678
                     /locus_tag="VGM46_13015"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEY4193560.1"
                     /translation="MNMQVTPPTDNRDVPAHETLSGAHPARRYVIGGLALVLVLGGFY
                     YWSHSGNGGPRRGPIVAPVRVANAVQRDMEVVEHTIGTVVANSTVSVTARVQGQLTKA
                     YFKEGQIVKEGDLL"
BASE COUNT          328 a          465 c          527 g          358 t
ORIGIN      
        1 aatgggcata tccacgttca ggtcgcacaa cgtatcggcg acagtggcat catccagacc
       61 ggcgatggcg acgatgtcgc cggcttcggc gcgttccacc ggcacgcggg cgaggccgcg
      121 gaaggccagc agcttggtga cgcgggtctt ctcaatgatc tcgccgtcgc ggttcaacgc
      181 cttgatgttg cggttggtgg tgatgacgcc ggattcgatg cggcccgtga ggatgcggcc
      241 caggaaattg tcagcctcca aagtggtgac cagcatcttg aagggatgct cttcgcccgc
      301 cagagcctgc ggcttcggcg ccggcacatt gtccacgatc agttggaaca gcggatcgag
      361 attcttgcgc tcgtcttcca gctccttcac cgcccagcca ttgcggccgg aggcatagag
      421 gtaatgaaag ttcagctgat cgtcattggc ttccagcgcc aggaacagat cgaaaatgga
      481 ttccagcgtt tccttgggct gggcgtcctg acggtcgatc ttgttgatga ccacgatggg
      541 cttgaggccc agcttcagcg ccttggagag cacgaatttg gtctgcggca tgacgctttc
      601 agccgcgtcc accagcagca ccacgccgtc caccatcgag aggatacgct ccacttcgcc
      661 gccgaaatcg gcatggccgg gggtgtccac cgtattgatg cgcgtgccat gccattcgac
      721 gctggtgcat ttggccagaa tggtgatgcc gcgttcgcgt tcctgatcgt tggaatccat
      781 ggcgcgctcg gccatgtgct ggttctcgcg caccgagccg gactgcttca gcagctgatc
      841 aaccagcgtg gttttgccat ggtcgacatg ggcgatgatg gcgatgttgc gaatattcat
      901 gaatcggggc gctcgggctg ggaaggccgg gcttatagac gccgcccccg tgacccgcaa
      961 tgcacgtcgc aaaagtcgca gcgccacaaa ataataacaa tgatttcaac gtgttaatga
     1021 ataattcggg cggttacatg cccccggggc ggtccttttt gagacaattg tgagagagtt
     1081 ttaccctata gagtgcgcaa gggtgagcgc cgaaataatg ctaaggccgt gcaaggcttg
     1141 aaaaaatcct cgtcatgggc gtgggataag cggaattttg ggcgtaaatt tttcgcactt
     1201 ggttgttaga agtgttgccg gtgtcaatac gggtttgggg aacgacattg cgtatcacgt
     1261 tgtcgcaaaa ttatgacagg gattgccgca agctcgcccc gcttgcgcag ttcggcttgg
     1321 gaaagatcgc atgaatatgc aggtaacgcc gccaacagat aatcgcgacg tgccggcgca
     1381 tgaaacgctg tccggtgcgc atcccgcacg ccgctacgtc atcggcggcc ttgcgctcgt
     1441 cctggtgctg ggcgggtttt actattggtc gcattccggc aatggcggcc cgcgccgcgg
     1501 tcccatcgtg gcgccggtgc gcgtcgccaa cgccgtgcag cgcgacatgg aagtggtcga
     1561 gcatacgatc ggaaccgtgg tcgccaattc caccgtgtcg gtgacggcgc gcgtgcaggg
     1621 gcagctcacc aaggcctatt tcaaggaagg ccagatcgtg aaggaaggcg atcttctg
//
LOCUS       DASYCS010000426        14867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1724959,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000426 DASYCS010000000
VERSION     DASYCS010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 14867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14867
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1724959"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(197..1042)
                     /locus_tag="VGM46_13020"
     CDS             complement(197..1042)
                     /locus_tag="VGM46_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY4193561.1"
                     /translation="MFRKFALAAAAALTALTIPLCGSAFAKDWKTVNVGVEGAFPPFN
                     ATTSSGGLEGLDLDVIKDLCKRAQLECNIVAQEWDSQIPSLLAGKFDVVLTMGPNPER
                     RKVIDFSNPYVITPNTFLVAADGPLAKLPHTGEKLSTDTEEGKKAVADLKEALKGKTM
                     GASLSTSQLTFVQQNFGDVVEVKTYKSSEQVQLDLESGRLDGQFDNIVFARDRAAKSN
                     GALVTSGPLLVGGIMATNVCLGIRKNEPELQAVLNKALAEMTADGTLAAMSQKWFGVD
                     LSPKG"
     gene            complement(1290..2216)
                     /locus_tag="VGM46_13025"
     CDS             complement(1290..2216)
                     /locus_tag="VGM46_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HEY4193562.1"
                     /translation="MNQAGSGNQATLGNPIPTWTWPEERLQKLMNRARAGKSLRPQAW
                     PGGAKCAVALSFDSDHDTFELRDGGRSISALSQGQFGPRQGMPRIRQLLDKNGIQATF
                     FVPAITALHYPDEQRTLIGEGHEIGLHGWIHERNTLLDADTERDLQHRAADTLERIAG
                     VRPVGIRTPSWDFSDNTLQITAEMGLLYDSSLMADVDCYEILLDGAPSGVVELPVEWI
                     RDDAAYLIMDRWGGLRPQIAPHDILKIFIAEFDAAYEEGGIFQLTMHPDIIGHRSRIW
                     LLGELIGHIRAKGNVWFATHADIVRHAKAAGQ"
     gene            complement(2213..2986)
                     /locus_tag="VGM46_13030"
     CDS             complement(2213..2986)
                     /locus_tag="VGM46_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY4193563.1"
                     /translation="MDVHFEGKRVLVTGAARGIGRAICAAFAAEGAAVVATDIIAGEL
                     AGLQDEIRPARGGALSVAQLDVTDEAAIDALVKNEDHAGGFDIFVHVAGGILGQTRKP
                     VEEVPASDWDRIYDVNVRGAFLVARSVVPAMKQRGAGKLVFISSGAGIGVSLTGIQAY
                     ASAKAALISLARQLGHELGPFGINVNAVAPGFLRTSPDYERQWASYGASGQADMLNQI
                     PLRRIGEPQDISNAVLFLASPFAGWITGQTLSVSGGPVQ"
     gene            complement(3003..3794)
                     /locus_tag="VGM46_13035"
     CDS             complement(3003..3794)
                     /locus_tag="VGM46_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEY4193564.1"
                     /translation="MNGESDSDVKSAVRVLEILELLARARKPLTLKMIVAELGYPKSS
                     TFNLLATLVSRAYVTRDEQETYRIHEAFRNGPGWLSGRDAQLIATAQPIMDALRDMEG
                     ETVFLGVRRKDGRVKSLAKSVSHQTVRFDTDLTGSDPSFCTAMGRILLANWSPEKTAA
                     YLARERIVHVTPHTVIDRIQIRRIIEAARVNGYAISDEEAVLGGSGVAAPVRDASGEV
                     IAALNIAAVSARFAASHERVLAAVIRNAAELSSRLGYKTTLTEEQ"
     gene            3877..4626
                     /locus_tag="VGM46_13040"
     CDS             3877..4626
                     /locus_tag="VGM46_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009762420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY4193565.1"
                     /translation="MAGSQKVAIVTGAARGIGKACAMDLAAAGYAIALVDVLEPELAD
                     TARKIEALGVPAAGFVADVSNYRRAGEVVAATVEAFGRVDFLLNNAGRSAPEGILDIS
                     EEQFDQAIAINLKSCFNYIQHAAPHMLAQGGGRIVSMSSLNALSGGVTAAVSRFAYAA
                     AKAGILGMTRALAKELGPSITVNAICPGVIETEVATNVTERADEVIKGIALGRLGTAD
                     DVARLVTFLATSQPCFMTGQTLTVDGFQFHV"
     gene            4681..5400
                     /locus_tag="VGM46_13045"
     CDS             4681..5400
                     /locus_tag="VGM46_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015343315.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY4193566.1"
                     /translation="MIDLLNLLSFGQEGWGLSLLTAALVTVAVAVCGFLIGTVIGALI
                     AWAKLAGNLPLRIGADAYTTILRGVPDLLVIYLFYFGGSLFTTWLFHLSGSEGFVGVP
                     PFVAGASAIGVIGAAYQAEVFRGAFRALSKGEIEAGMSVGMSRFLLFRRIIAPQVVQY
                     ALPGIGNVWQMALKESALISVTGLVELLRQSQIAAGSTRKPFEFYLIAGAIFLVITWL
                     SQQIFAWLDRRASRSLRREVR"
     gene            5400..6125
                     /locus_tag="VGM46_13050"
     CDS             5400..6125
                     /locus_tag="VGM46_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4193567.1"
                     /translation="MELSFIRDSFVQLLAGAPLTLELTAIAFAIGVVLAMLLALMRLS
                     RFAVIRGAADAYVFVIRGTPLLVQIFLIYYGLGQFRATLQSLGLWYFFREAYWCGILA
                     LSLNSAAYGSEVVRGALASVSLREIEAAKSCGMSGMLLYRRIILPLALRQALPAYGNE
                     LILMVKATSLVSIITMMEITGIAARLIADTYRPVEVFIAAGAIYLLINFVLTRLVALL
                     DWWLTPHLRAAASPSARLESSNG"
     gene            6118..6903
                     /locus_tag="VGM46_13055"
     CDS             6118..6903
                     /locus_tag="VGM46_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012092930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY4193568.1"
                     /translation="MAEPENPTPVVSLRGLRKSFGALDVLKGISLDAWKGDVIAILGS
                     SGSGKSTMLRCINLLETPSAGEISIGGETVKLKKHGHELAIADQRQVDALRTKTAMVF
                     QSFNLWSHMTILENLIEAPVHVQHRPKPEAVAEAMALLEKVGIADKCDAYPSHLSGGQ
                     QQRAAIARALAMHPQVMLFDEPTSALDPERVGEVLKVIRTLAGEGRTMLVVTHEIGFA
                     REVANRVVFLHQGVIEEDGPAETVFNHSKSERFRSFIANTRNS"
     gene            6932..8101
                     /locus_tag="VGM46_13060"
     CDS             6932..8101
                     /locus_tag="VGM46_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007564767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEY4193569.1"
                     /translation="MTIGRDTEILAWLAARQDAMVALLGELVNIDSGSYDKAGVDAVG
                     ARLIAFLAEHDIATGVLPVRDFGDTLLAGTASVRRDGERRNFLLLGHRDTVFGKGEAA
                     RRPFRVSGDRAYGPGVADMKAGLVMNAFILAAFQRFSPHIPVVAMMTGDEEIGSQASL
                     AHIEAEARFARAVFNAEPARVSGNFVSGRKGGFTYRFEIEGKAAHAGVNFTEGASAIG
                     EMAHKIAALHALTDVAAGMTLNVGLVSGGSSTNTVAASAAAELDVRFVDNAQREHLIR
                     QIEAILTTSSVEGTATSFERQSESLPLAPTPRNARLCACYAEAARELGLEIEGEYTGG
                     CADSGITSSAEAPTVCATGPVGGKAHTQDEYIVCATLSERARVVAATIEALAGEA"
     gene            8286..9908
                     /locus_tag="VGM46_13065"
     CDS             8286..9908
                     /locus_tag="VGM46_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEY4193570.1"
                     /translation="MIRLENISKQNGKQIVFIEASAALQRREKIGLVGPNGAGKTTLF
                     RMITGEELPDEGQVSVDRGVSIGYFSQDVGDMSGRSALVEVMDGVGPVSALAAEMAEL
                     EHAMGDPERAGEMDDIFARYSEVQVQFDELDGYALDGRAREVLGGLGFSQEMMDGDVG
                     KLSGGWKMRVALGKILLMQPDVMLLDEPSNHLDLESLIWLENFLKGFDGALLMTSHDR
                     EFMNRIVGKIIEIDGGTLTSYSGDYDFYRQQRAIAEVQQQAQFERQQAMLAKEIAFIE
                     RFKARASHAAQVQSRVKKLEKIDKVEPPKRQQTVRFEFQPAPRSGEDVATLKSVHKSY
                     GSRKIYEGLDFQVRRRERWCVMGVNGAGKSTLLKLIAGMSEPDGGSVARGPSVKMGYF
                     AQHAMELLDGEQTVLQSLEDSFPQAGQAPLRALGGCFGFHGDEVEKKCRVLSGGEKAR
                     LVMAKMLFDPPNFLVLDEPTNHLDIATKQMLIEALSRYEGAMLFVSHDRHFLAALSNR
                     VLELTPEGLRTYGGGYTEYVERTGQEAPGLRS"
     gene            complement(9937..11037)
                     /locus_tag="VGM46_13070"
     CDS             complement(9937..11037)
                     /locus_tag="VGM46_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018210803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ionic transporter y4hA"
                     /protein_id="HEY4193571.1"
                     /translation="MAEHSHTSIPAWTWIAPLLAAVLLALKFGHVVSGDAVPVLVLSA
                     ILLAAAVFAAVHHAEVLAVKLGEPFGSILLAVAVTVIEVALIVSIMLAGALGSETVAR
                     DTIFAAVMIVLNGVIGLCLIMGGRRHFEQTFQLQGASSALAVLGTLAALTLILPNYTL
                     TVSGPSFSATQALFIGAVSLVLWGVFVFVQTVRHRDYFLDAAEAETTELPVEKPSDRI
                     AIACLGLLLLSLVAVILLAKVLSYPLDKAVAAAGLPQAFVGVVIAAIVLLPEGVAAAK
                     SAVRNRLQNSINLALGSAIASIGLTIPTVAVVALLLKQDLILGVSPTNITLLVLTLFV
                     GTLTLGTGRTTVLQGVIHLVIFTVFLLVSALP"
     gene            complement(11331..12710)
                     /locus_tag="VGM46_13075"
     CDS             complement(11331..12710)
                     /locus_tag="VGM46_13075"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEY4193572.1"
                     /translation="MAAIRHVAVIDIGKTNAKVALVDLDTLSETAVRKTANRPIAGAP
                     YRRHDVEALWDFILRSLGELGREQPVDAISVTTHGATAALLDADGALALPVLDYEDEG
                     PATRRERYDAVRPPFGETGSAPLPNGLNLGAQLFWQSQAFPEAFARVSTVLTYPQYWG
                     WRLTGIAASEVTSLGCHTDLWDPYRGGFSTLVEALAWRALFAPLRPASARLGMLRADL
                     AARTGLDPSTPVYNGIHDSNASLLPHLLARPAPFAVVSTGTWVVAMAIGGHETPLDPA
                     RDTLVNVNALGDLVRSARFMGGREFEMLAAGADASINEARDDEAVSSVLAGAIMLLPS
                     VQPGSGPFPAGKARWVGAPSPRECAVAVDFYLALMTATCLDLIGADGPVIIEGPFAAN
                     RNYCDMVQVATGRAVLAQAGQTTGTSIGAALLADMSRMPPAPTASVKDYPPAWRRYAQ
                     EWRAAANAG"
     gene            complement(12713..13030)
                     /gene="rhaM"
                     /locus_tag="VGM46_13080"
     CDS             complement(12713..13030)
                     /gene="rhaM"
                     /locus_tag="VGM46_13080"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HEY4193573.1"
                     /translation="MAEKYAFRLRLNPGMKSEYKRRHDAIWPELSALLKDAGISDYSI
                     HLDEETGLLFGVLWRREGHAMADLPSHPVMRKWWAYMADLMETGPDGAPVAARLETVF
                     HMA"
     gene            complement(13030..14031)
                     /locus_tag="VGM46_13085"
     CDS             complement(13030..14031)
                     /locus_tag="VGM46_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015319237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4193574.1"
                     /translation="MTDTPAPRRIPDRLDKPLKSAIFSWEALLVTVALAIFVANSLAS
                     PYFLDPWSLSDLTFNFTEKGLIALAMALLIIAGEIDLSVAAIIALASTLMGMAAQMGV
                     GTPGLVLIGLAVGLACGAFNGLLVTGLGLPSIVVTIGTMSLYRGIAFIILGDQAYKGY
                     PPSFAFFGQGYVWWVVSFELVLFLGAAVVYRALLHRTSFGRRVFAIGNNPVAAQFSGV
                     RVGRIKFILFCLTGLMSGIAGILITSRLGSTRPSIAQGYELEVITMVVLGGVNILGGA
                     GSIAGVVLAAFIMGLVTFGLGLLNVPGIVMSIFIGLLLIVVIALPILWRRLRRKRPA"
     gene            complement(14028..>14867)
                     /locus_tag="VGM46_13090"
     CDS             complement(14028..>14867)
                     /locus_tag="VGM46_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006204567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4193575.1"
                     /translation="MILALGQMTVILTRAIDLSMAANLCFTGMVVAMLNATHPAIPIP
                     VLIVVALAVGLALGAINGLLVWRLNIPSIVVTLGTLTIYRGATFVLSGGAWVNADQMS
                     QPFINFQRAVLLGVPVLSWFAIAVIAVFFVLMTRTALGRSIHAAGVNPTASVYAGIDV
                     GRTQFVAFCISGAVSGLAGYLWISRYVIASVEVANGYELNIIAACVIGGISIAGGIGS
                     VGGAVLGSLFLGIIANALPVINISPFWQMAISGAAIILAVVLNARGERRPGRIILKKA
                     EAA"
BASE COUNT         2753 a         4726 c         4817 g         2571 t
ORIGIN      
        1 ggaaaacctg cctgcttcat cgccgctata cgccgccggc cggcctgtga cgcgccgacc
       61 gtgcctgcca tcggtatcga ccgacggctc cccgccatcg gcatcgttgg tcaccggcct
      121 gatccggggg ccggctggtc atgggaaaat ttgcgccagc cgggcgacgg tcttggtccg
      181 cctcgcgaaa aattactcag cctttcgggc tgagatcgac gccgaaccac ttctgggaca
      241 tggctgccag cgtgccgtct gccgtcattt cggccagcgc cttgttgaga accgcctgga
      301 gttccggctc gttcttgcgg atgccaaggc agacattggt ggccatgatg ccgccaacca
      361 gcagcggtcc gctggtgacc agcgcgccat tgctctttgc ggcgcggtcg cgcgcgaaca
      421 cgatgttgtc gaactggccg tccagacgcc cgctttcgag atcgagctga acctgttcgg
      481 acgacttgta ggtcttgacc tcgaccacat cgccgaaatt ctgctggacg aaggtcagct
      541 ggctggtcga gagcgacgcg cccatcgtct tgcccttcaa ggcttcctta aggtcggcaa
      601 ccgccttctt gccttcttcc gtgtcggtcg agagcttctc gccggtgtgc ggcagctttg
      661 ccagcggacc gtcggcggcg accaggaacg tattcggcgt gatgacgtaa gggttggaga
      721 aatcgatcac cttgcggcgc tccgggttcg gacccatggt caagacgaca tcgaacttgc
      781 cggccagaag cgacgggatc tggctgtccc attcctgggc gacgatgttg cattccagct
      841 gagcgcgctt gcacaggtcc ttgatcacgt cgagatcgag accttccagg ccgccgctgg
      901 aggtggtggc gttaaagggc gggaaggcgc cttcgacgcc gacattgacg gtcttccaat
      961 ccttggcgaa ggcgctgccg caaaggggga tggtcaaggc agtcagggcg gcggccgccg
     1021 ccaaagcgaa tttcctgaac atgggttcct cacattttga acgatagagt gctcaaggtt
     1081 cagtggagca ggtcttgtcg ccgttgccct gagcttacct aattttgaac gtacttatga
     1141 aagctgaaaa gcgaaacatc gttcacatac gggaattacg ttcagaaaac tgcgcgtctt
     1201 tcgggcaatg tgcggccagg atcgcagcct gggccttggc cggcacgtcc atagccggcc
     1261 aggcgacgct tgcgcggtta gcggcgtttc tattgccccg ccgccttggc gtggcgcacg
     1321 atatcggcat gggttgcgaa ccagacattg cctttggcgc ggatatggcc gatcaattcg
     1381 ccgagcagcc agatgcggga gcggtggccg ataatgtcgg ggtgcatcgt cagttggaaa
     1441 atgccgcctt cttcgtaagc ggcatcgaat tccgcgatga atatcttcaa aatatcatgc
     1501 ggcgcgatct gcggccgcaa tccgccccag cgatccatga tcagataagc ggcgtcgtcg
     1561 cgtatccatt cgacaggcag ttcgaccacg ccggagggcg cgccgtcgag caggatctcg
     1621 taacagtcga catcggccat cagcgaggaa tcgtagagca ggcccatttc cgccgtgatc
     1681 tggagcgtgt tgtcgctgaa atcccacgat ggcgtgcgga tgccgaccgg gcgcacgccg
     1741 gcgatgcgct ccagcgtgtc ggcggcgcgg tgttgcaggt cgcgctcggt gtccgcatcg
     1801 agcagcgtgt tgcgctcatg gatccaccca tgcaggccga tctcgtggcc ttcgccgata
     1861 agcgtccgct gctcgtcggg atagtgcagc gcggtgatgg ccggcacgaa gaatgtcgcc
     1921 tggatgccgt ttttatccag caattgccga atgcgcggca tgccctggcg tggcccgaac
     1981 tggccttgcg aaagcgcgga tatggaccgg ccgccatcgc gcagttcgaa ggtgtcgtgg
     2041 tcggaatcga acgacagcgc caccgcgcat ttggcgccgc ccggccatgc ctgcggccgc
     2101 aggctcttgc cggcgcgcgc gcggttcatc agtttttgca ggcgctcctc cggccacgtc
     2161 caggtcggga tcggattgcc tagtgttgct tggttccctg atcctgcttg gttcattgta
     2221 ccggtcctcc gctgaccgaa agagtttggc cggtgatcca gccggcgaat ggcgacgcca
     2281 gaaacaggac ggcattggaa atatcctgcg gctcgccgat gcggcgaagc gggatctggt
     2341 tcagcatatc cgcctggccg gaagcgccgt aggatgccca ttggcgctcg taatccgggc
     2401 tggtgcgcag gaagccgggc gccaccgcgt tgacattgat gccgaacggc ccaagctcat
     2461 ggccaagctg gcgggccagg ctgatgagcg ccgccttggc gctggcatag gcctggatgc
     2521 cggtgaggct gacgccgatg ccggcgccgc tggaaatgaa caccagcttg cccgcgccgc
     2581 gctgcttcat ggcgggtacg accgagcggg ccacgagaaa ggcgccgcgc acgttgacgt
     2641 cgtagatgcg gtcccagtcg ctggccggca cttcctcgac cggctttcgc gtctggccta
     2701 aaatgccgcc ggcgacatgc acgaaaatgt cgaaaccgcc tgcatggtcc tcgtttttga
     2761 ccagcgcgtc gatggcggct tcatcggtca cgtccaactg cgccacgctc agcgcgccgc
     2821 cgcgcgccgg gcggatttca tcttgaagac cggccaactc gccggcgatg atgtcggtgg
     2881 cgacgaccgc cgcgccctcg gcggcgaacg ccgcgcagat cgcccggccg atgccacggg
     2941 cggcgccggt gaccagaacc cgttttccct cgaagtgaac gtccatctgc tgctcttttg
     3001 tcctactgct cttccgtcaa tgtggtcttg tagccgaggc ggctgctgag ttcggccgcg
     3061 ttgcggatca ccgcggcaag cacgcgttcg tggctggcgg cgaagcgcgc ggaaacggcg
     3121 gcgatgttga gggcggcgat gacctcgccc gacgcatcgc ggacgggggc ggcgacgccc
     3181 gagccgccaa ggacggcctc ctcgtcgctg atggcgtagc cgttgacgcg cgcggcttcg
     3241 atgatgcggc gaatttgtat cctgtcgatc accgtgtgcg gcgtgacgtg cacgatgcgc
     3301 tcgcgggcaa gataggcggc ggtcttttcc gggctccaat tggccaaaag gatgcgtccc
     3361 atcgccgtgc agaaggaagg gtcggacccg gtgaggtcgg tgtcgaagcg aaccgtctgg
     3421 tggctgacgc tcttggccag cgacttgacc cggccatcct tgcggcgcac gccaagaaaa
     3481 accgtctcgc cttccatgtc gcgaagcgca tccatgatcg gctgcgccgt cgcgatcagc
     3541 tgcgcgtcgc gcccgctcag ccagcccggc ccgttccgga aagcctcgtg tatccggtac
     3601 gtctcctgct cgtcgcgcgt gacataggcg cgcgaaacca gcgtcgccag caggttgaag
     3661 gtgctgctct tgggatagcc cagctcggca acgatcatct tcagcgtgag aggcttgcgg
     3721 gcgcgcgcca gcagttcgag aatttcgagc acccgcaccg ccgacttaac gtcagaatcg
     3781 ctttcaccgt tcatatatat gaaccatatt cctattgaag aatgcaatca ccgccatatc
     3841 atttccgccg atagttgaaa aggatcagtt tcagaaatgg ccggatcaca aaaggtggcg
     3901 atcgtcacag gcgcggcgcg cggcatcggc aaggcctgcg ccatggatct cgccgccgca
     3961 ggatatgcca tcgcgctggt cgacgtgctg gagccggagc ttgccgacac ggccaggaag
     4021 atcgaggcgc tcggcgttcc ggcagccggc ttcgtcgccg atgtttcgaa ctacaggcgg
     4081 gccggcgaag tggtggcggc gacggtcgag gcgttcggcc gtgtcgactt ccttttgaac
     4141 aatgccggcc gttccgcgcc ggaaggcatt ctcgacattt ccgaggaaca gttcgaccag
     4201 gcgatcgcca tcaatttgaa aagctgcttc aattacatcc agcacgccgc gccgcacatg
     4261 ctggcgcagg gcggcgggcg catcgtgtcg atgtcgtcgc tcaacgcgct ttccggtggc
     4321 gttacggcgg cggtcagccg atttgcctat gcggcggcca aggccggtat cctgggcatg
     4381 acccgcgccc tggccaagga actggggccg agcatcacgg tcaacgccat ctgccccggc
     4441 gtgatcgaga ccgaagtggc gacaaacgtg acggagcgcg ccgacgaagt gatcaagggc
     4501 atcgcgcttg gacggcttgg caccgccgac gacgtggcaa ggctggttac gttcctggcc
     4561 acctcgcagc cctgcttcat gaccggccag accctgacgg tcgacgggtt ccagttccac
     4621 gtttgaggaa cggcagaacg ttccgggaga aattgactgg ccgggaggaa ttgactgggg
     4681 atgattgatc ttctgaactt gctgagcttc gggcaggaag gctggggcct ctcgctgttg
     4741 acggccgccc tggtgaccgt cgccgttgcc gtttgcggct ttctcatcgg cacggtcatc
     4801 ggcgcattga tcgcctgggc gaagctcgcc ggcaacctgc cgctcaggat cggcgcggac
     4861 gcctacacca ccattctgcg cggcgtgccc gatcttctgg tcatctatct tttctatttt
     4921 ggcggcagcc tgttcaccac ctggcttttc catctctcgg gcagcgaggg gtttgtcggc
     4981 gttccgccct tcgtcgccgg ggcaagcgcc atcggcgtca tcggcgcggc ctatcaggcc
     5041 gaggtctttc gcggtgcgtt tcgcgcgctg tcgaaaggcg agatcgaggc gggcatgtcg
     5101 gtgggcatga gccggttctt gctgtttcgg cgcattatcg cgccgcaagt cgtgcaatat
     5161 gcgctgcccg gcatcggcaa tgtctggcag atggcgctca aggaatcggc gctgatttcc
     5221 gtcaccggac tggtcgagtt gttgcggcag tcgcagatcg ccgccggttc gacgcgcaag
     5281 ccgttcgaat tctacctgat cgccggcgcc atcttcctgg tcatcacctg gctgtcgcaa
     5341 cagatttttg cctggctgga ccggcgcgcc tcgcgcagcc tgcggcggga ggtgcgctga
     5401 tggaattgtc cttcatccgc gacagcttcg tccagttgct cgccggcgcg ccgctgacgc
     5461 tcgaactcac cgccatcgcc tttgccatcg gggtggtgct ggcgatgttg ctggccttga
     5521 tgcggctttc ccgcttcgcc gtcattcgcg gcgccgccga cgcctatgtg ttcgtcattc
     5581 gcggcacgcc gcttctggtg caaatcttcc tgatctatta cgggcttggc cagttccggg
     5641 cgacgctgca aagccttggc ctgtggtact tcttccgcga ggcatattgg tgcggcatcc
     5701 tcgcgctttc gctcaacagc gccgcctatg gcagcgaggt cgtgcgcggc gcgctcgcat
     5761 cggtgtcgct gcgcgaaatc gaggcggcca agtcatgcgg catgtccggc atgctgctct
     5821 accggcgcat catcctgccg ctggccctgc ggcaggccct gccggcctac ggcaacgaac
     5881 tcatcttgat ggtaaaggcg acctcgctcg tctcgatcat caccatgatg gagatcaccg
     5941 gcatcgcggc gcggctcatc gccgacacct atcgcccggt cgaggtcttc atcgcggccg
     6001 gggcgatcta tcttctcatc aacttcgtgc tgacgcgcct ggtggcgctg ctcgactggt
     6061 ggcttacccc gcatctgcgg gcggcggcct cgccgagcgc cagactggag agttccaatg
     6121 gctgaaccgg aaaatcccac gcctgtcgtg tcgctgcgcg gcctgcgcaa gagctttggc
     6181 gcgctcgacg tgctgaaggg catctcgctc gatgcatgga agggcgacgt cattgcgata
     6241 ttggggtctt ccgggtcagg caaaagcacg atgctgcgct gcatcaacct gctggaaacg
     6301 ccgagcgccg gcgaaatcag cattggcggc gaaacggtga agctgaagaa gcacggccat
     6361 gaactggcca tcgccgacca gcggcaggtc gacgccctgc gcaccaagac cgcgatggtg
     6421 tttcagagtt tcaatctgtg gtcccacatg accatcctcg aaaacctcat cgaagccccc
     6481 gtccatgtcc agcatcggcc gaaacccgaa gcggttgccg aagcgatggc gctgcttgaa
     6541 aaggtcggca tcgccgacaa atgcgacgcc tacccgtccc acctttccgg cggccagcaa
     6601 cagcgcgcag cgattgcgcg cgcgctcgcc atgcatccgc aggtcatgct gttcgacgag
     6661 ccgacctcgg cgctcgaccc cgagcgcgtc ggcgaggttt tgaaggtcat ccgaacgctg
     6721 gccggcgaag gccgcaccat gctcgtcgtc acccacgaaa tcggctttgc ccgcgaagtc
     6781 gccaaccggg tcgtcttcct gcaccagggc gttatcgagg aagacggccc cgccgagacg
     6841 gtcttcaacc attccaaatc ggagcgcttc aggagcttca ttgccaacac ccgcaattcc
     6901 tgaccagcaa acacatgaag gcgaaggcgc aatgaccatc ggtcgagaca cggaaatact
     6961 ggcctggctg gccgcccggc aggacgcgat ggtcgcgttg ctgggcgaac tcgtcaacat
     7021 cgattccggc tcctacgaca aggcgggagt cgatgcggtc ggcgcgcgcc ttatcgcatt
     7081 cctcgccgag catgacatcg ccaccggcgt gttgcccgtc cgcgacttcg gcgacacgct
     7141 cctggccggc acggcctccg tgcgccggga tggcgaacgc cgcaatttcc tgctgcttgg
     7201 ccaccgcgac accgtgttcg gcaaaggcga ggcggcgcgc cggcccttca gggtttcggg
     7261 cgaccgggcc tatggccccg gtgtcgccga catgaaggcg gggcttgtga tgaacgcctt
     7321 catcctcgcg gcattccagc gtttctcccc tcacatcccc gtggtggcga tgatgaccgg
     7381 agacgaggag atcggctcgc aggcgtcgct tgcccatatc gaggccgagg cgcgttttgc
     7441 aagggcggtg ttcaacgcgg agccggcgcg cgtttcggga aacttcgtca gcggccgcaa
     7501 gggcggcttc acctatcgtt tcgagatcga gggcaaggcg gcccatgccg gcgtcaactt
     7561 caccgaaggg gccagcgcaa tcggcgaaat ggcgcacaag atcgcggcgc tgcacgccct
     7621 gaccgatgtt gcagccggca tgacgctgaa tgtcgggctg gtcagcggcg gctcatcgac
     7681 caacacggtg gcggcgtcgg cggcggccga actcgacgtg cgcttcgtcg ataacgcgca
     7741 gcgcgaacac cttatccgcc agatcgaggc gatcctgacg acctcatcgg tagaaggaac
     7801 cgcgacctcc ttcgaacgcc agagcgaatc gctgccgctt gcgccgacgc cgcgcaacgc
     7861 gcgcctgtgc gcatgctacg ccgaggcggc aagggaattg gggctggaaa tcgagggcga
     7921 atataccggc ggctgcgcgg attccggcat cacctccagc gccgaagcac ccacggtctg
     7981 cgcaacgggg ccggtcggcg gcaaggcgca tacgcaagac gagtatatcg tctgcgccac
     8041 gctgtccgaa cgcgcgcgcg tggttgccgc gacgatcgag gccctggccg gcgaagcgtg
     8101 agccgcggca ggcctggcgc ggctgcttga ccgctgggcc gacgccgctg gagggctatc
     8161 ctcgcacatc tcgaagccgg ataatccggt tgcaatatcg acccaccttg cgttgccgcg
     8221 cccgcaatgg tttagcggcg gcagttgcgc ggacgcctcg cacagccctg cacattggaa
     8281 gcccgatgat ccgcctcgaa aatatcagca agcagaacgg caagcagatt gtcttcatcg
     8341 aagcgtccgc cgcgctgcaa cgccgcgaga agattggcct cgttggcccc aatggcgcgg
     8401 gaaagaccac ccttttccgc atgatcaccg gcgaggaact gcctgacgaa ggccaggttt
     8461 ccgtcgatcg cggcgtcagc atcggatatt tcagccagga tgtgggcgac atgtccggcc
     8521 gcagcgcgct cgtcgaggtg atggacggcg tcggcccggt aagcgcgctg gccgccgaaa
     8581 tggccgagct ggagcacgcg atgggcgatc ccgaacgcgc cggcgagatg gacgacatct
     8641 tcgcgcgcta cagcgaagtg caggttcaat tcgacgagct tgacggctat gcgctggacg
     8701 gcagggcgcg cgaggttctc ggcggcctcg gcttcagcca ggaaatgatg gacggcgacg
     8761 taggcaagct ttccggtggc tggaagatgc gcgtcgcgct gggcaagatc ctgctgatgc
     8821 agccggacgt gatgctgctt gacgagccga gcaaccatct cgacctggaa agcctgatct
     8881 ggctggagaa cttcctgaaa gggttcgacg gcgcgctttt gatgacctcg cacgaccgcg
     8941 agttcatgaa ccgcatcgtc ggcaagatca tcgagatcga cggcgggacg ctgacctcct
     9001 attcgggcga ctacgacttc taccgccagc agcgcgccat cgccgaagtg cagcagcagg
     9061 cgcagttcga gcgccagcag gcgatgctcg ccaaggaaat cgcctttatc gagcgtttca
     9121 aggcgcgcgc ctcgcatgcc gcgcaggtgc agagccgcgt caagaaactg gaaaagatcg
     9181 acaaggtcga gccgcccaag cgccagcaga cggtgcgctt cgagttccag ccggcgccgc
     9241 gctcgggcga ggatgtcgcg acgctgaaaa gcgtccacaa gagctatggc agccgcaaga
     9301 tttacgaggg cctcgatttt caggtgcgcc ggcgcgagcg ctggtgcgtg atgggcgtca
     9361 acggggctgg aaaatcgacg cttctgaagc tgatcgccgg catgtcggag ccggatggcg
     9421 gctcggtggc gcgcgggccg agcgtcaaga tgggctactt cgcccagcat gccatggaat
     9481 tgctggatgg cgagcagacg gtgctgcaat cgctggagga ttccttcccg caggccggac
     9541 aggcgccgtt gcgcgcgctg ggcggctgct tcggcttcca cggcgacgag gtcgagaaga
     9601 aatgccgcgt gctttcaggc ggcgaaaagg cgcggctggt catggccaag atgctgttcg
     9661 atccgccgaa cttcctcgtt ctcgacgagc cgaccaacca tctcgacatc gccaccaagc
     9721 agatgctgat cgaggcgctg tcgcgctatg agggcgcgat gctgttcgtc agccatgatc
     9781 gccatttcct cgccgccctg tccaatcgcg tgctggaact gacgcccgaa gggctgcgca
     9841 cctatggcgg cggctacacc gaatatgtcg agcgcaccgg ccaggaagct ccgggcctgc
     9901 gcagctgatt tggcggaaag cgccccggca ggccggtcag ggcagcgccg acaccagcag
     9961 gaacacggtg aagatcacca gatggatcac gccttgcagc actgtcgtgc ggccggtgcc
    10021 cagcgtcagc gtgccgacga acaacgtcag caccagcagc gtgatgttgg tcggcgagac
    10081 gccgagtatc aggtcctgct tgagcagcag cgcgacgacc gcgacggtgg gtatggtcaa
    10141 gccgatgctg gctattgccg agccgagcgc caggttgatg ctgttctgga gccggttcct
    10201 gacggcggac ttcgccgccg ccacgccctc gggcaacagc acgatcgcgg caatgaccac
    10261 gccgacgaac gcctgcggca gcccggccgc ggcaaccgcc ttgtccagcg ggtaggacag
    10321 caccttggcc agcaatatca ccgccaccag cgacaatagc agcaatccga gacaggcgat
    10381 cgctatccgg tcgctgggct tttcgacggg cagttcggta gtttcggcct ccgccgcatc
    10441 gaggaaataa tcgcggtgcc ggaccgtctg gacaaacacg aaaacgcccc acagcaccag
    10501 cgacacggcg ccaatgaaca aggcttgcgt agccgagaaa gacgggccgg aaacggtcag
    10561 cgtataattg ggcaggatca gtgtcagcgc ggcaagcgtg cccagaacgg ccagcgccga
    10621 agaggcgccc tgaagctgga aagtctgttc gaaatgccgc cttccgccca tgatgaggca
    10681 cagcccgatg acgccgttca gcacgatcat cacggccgcg aagatcgtgt cgcgcgccac
    10741 cgtttcgctc ccaagcgccc cagcaagcat gatcgagacg atcagcgcca cctcgatcac
    10801 ggtcacggcg acggcaagca ggatcgagcc gaacggctcg cccagcttga cggccagaac
    10861 ctcggcgtga tgcacggccg cgaaaaccgc cgctgcaagc aggatggcgg agagaaccag
    10921 cacaggtacg gcgtcgccgg aaacgacatg gccgaatttg agcgcaagca ggacggccgc
    10981 cagcaagggg gcgatccagg tccaggcggg gatcgaggtg tgactatgct cggccattat
    11041 ccgtggttcc ctccctgatg ccgcctgccg ggccgtcgcc ccgacgatcg catcgagcgt
    11101 tcgcggcggc ccacatttcg cttatccgaa aatgcacagc gctcctagag cggttctggt
    11161 ttttacctaa tcgacaaagc cgttctatct ctttgttttg gccgcatttc cggacgcaaa
    11221 accgttgcac acttttgctg gaaatgctcc tagtcaccat cgaaacatac atgctgctcg
    11281 gggcgaagag ggccggaacc cttattgaag ccgccgaatg caaagcctgc ctagccggcg
    11341 tttgccgccg cccgccactc ttgcgcataa cgccgccagg caggtggata gtccttcacg
    11401 ctcgccgttg gcgccggcgg catccggctc atgtcggcca gcagggctgc gccgatgctg
    11461 gtgccggtgg tttggccggc ctgggcgaga accgcccgcc ccgtcgccac ctgcaccatg
    11521 tcgcaataat tccggttggc cgcgaacggg ccttcgatga tgacaggccc atccgcgccg
    11581 atcaggtcga ggcaggtcgc ggtcatcaag gcgaggtaga aatctaccgc gacggcgcat
    11641 tcacgcggcg aaggcgcgcc gacccagcgt gccttccccg ccgggaacgg accggaaccc
    11701 ggctggaccg agggcagcag catgatggcg cctgcaagca ccgacgagac ggcctcgtcg
    11761 tcgcgcgctt catttatcga ggcgtctgct ccggcagcca gcatttcgaa ctcgcgtccg
    11821 cccatgaagc gggccgaacg cacgagatcg ccaagcgcgt tgacattgac cagcgtgtcg
    11881 cgcgccgggt cgagcggcgt ttcgtggccg ccaatcgcca ttgcgacgac ccatgtgccg
    11941 gtcgagacga ccgcgaacgg agccggccgc gccaacaggt gcggcagcag ggaagcgttg
    12001 gaatcgtgga tgccgttata aaccggcgtc gatggatcga ggccggtccg cgccgcaaga
    12061 tcggcccgca acatccccag ccgggcgctt gccgggcgca gcggcgcaaa caatgcccgc
    12121 cacgccaagg cctcgacaag cgttgaaaac ccgccgcggt aggggtccca caaatcggtg
    12181 tggcagccaa gcgaggtcac ttcgctggcc gcaatgccgg tcagccgcca tccccaatat
    12241 tgcggatagg tcaggaccgt cgaaacccgc gcaaacgcct ccggaaaggc ctggctctgc
    12301 cagaaaagct gcgcgccgag gttgaggccg ttcggcagcg gcgcggagcc ggtttcgccg
    12361 aacggcggcc gcacggcatc gtagcgctca cgccgcgtgg ctgggccttc gtcctcatag
    12421 tcgagcaccg gcagggcaag cgcaccgtcg gcatcgagca aagccgccgt cgcgccatgg
    12481 gtggtgaccg agatggcgtc gacaggctgc tcccgcccca gttcgccaag gctgcgcaaa
    12541 atgaaatccc acagcgcctc gacatcgtga cgccggtacg gagcgcctgc gatcgggcgg
    12601 tttgccgtct tgcgaacggc cgtttccgac agcgtgtcga ggtcgacaag ggctaccttg
    12661 gcattggtct tgccgatatc gatgacggcg acgtggcgga tggcggccat gctcatgcca
    12721 tatggaagac ggtttcgagc cgcgccgcca ccggcgcgcc gtccggcccg gtttccatca
    12781 ggtcggccat ataggcccac catttccgca tgaccggatg gctgggcaaa tccgccatgg
    12841 cgtggccttc ccgacgccac agaacgccga acagcaggcc ggtttcctcg tcgagatgaa
    12901 tggaataatc cgaaatgcca gcgtctttca gcagcgctga aagctccggc caaatcgcat
    12961 cgtgccggcg cttgtactcg cttttcatgc cggggttcag ccgcagccgg aaagcatatt
    13021 tttcggccat caggcgggcc ttttcctgcg tagccgccgc cacaatatgg gcagcgcgat
    13081 gacgacgata agcaaaagcc cgatgaagat cgacatgacg atgccgggca cgttcaagag
    13141 gccaagcccg aaagtgacca gccccatgat gaaggcggcg agcacgacgc cggcgatgct
    13201 gcccgcgcca cccagaatat tgacgccgcc cagcaccacc atggtgatga cttccagttc
    13261 gtagccctgc gcgatcgatg gccgcgtcga gccgaggcgc gaggttatca ggatgccggc
    13321 gatgccggac atcagcccgg tcaggcaaaa caggatgaac ttgatgcgcc cgacgcgcac
    13381 gcccgaaaac tgcgcggcaa ccgggttgtt gccgatggcg aagacccgcc ggccaaagct
    13441 ggtccggtgc agcagcgccc gatagacgac cgccgcgccg aggaacagca ccagttcgaa
    13501 cgacaccacc caccagacat agccctggcc gaaaaaggcg aaacttggcg gatagccctt
    13561 gtaggcctgg tcgcccagga tgatgaaggc gatgccgcga tagaggctca tcgtgccgat
    13621 ggtcaccacg atggaaggca ggccaaggcc tgttaccaga agaccgttga acgccccgca
    13681 ggcgaggccg accgcgaggc cgatcagcac gaggccgggc gtgccgacgc ccatctgcgc
    13741 cgccatgccc atcagcgtcg aggcgagcgc gatgatggcg gcgacggaaa ggtcgatctc
    13801 gccggcgatg atcagcagcg ccatcgccag cgcgatcagg cccttttcgg tgaaattgaa
    13861 ggtaaggtcc gacagcgacc acggatcgag gaaatagggc gaggcaaggc tgttggcgac
    13921 gaagatggcg agcgcaacag tcaccagcag cgcctcccat gagaagatcg cggatttgag
    13981 cggcttgtcc agccggtcgg gaatgcggcg cggggcgggc gtatcggtca tgccgcttcg
    14041 gccttcttca ggatgatgcg acccggccgg cgttcaccgc gtgcgttgag gacgacggcg
    14101 aggataatgg cggccccgga aatcgccatc tgccagaacg gcgagatgtt gatgaccggc
    14161 agcgcgttgg cgatgatgcc gagaaacagc gagcccagca ccgcgccgcc gaccgagccg
    14221 atgccgccgg caatcgaaat cccgccgatg acgcaggcgg cgatgatgtt cagctcatag
    14281 ccgttggcga cctcgaccga ggcgatgacg tagcgggaaa tccacagata gccggccagc
    14341 ccggaaaccg ccccggaaat gcagaacgcc acgaattgcg tgcgcccgac atcgatgccg
    14401 gcatagacgg atgccgtcgg gttgacgccg gccgcgtgga tcgaccggcc aagcgccgtg
    14461 cgcgtcatca agacgaagaa aaccgcgatg acggcaatgg cgaaccacga cagcaccggc
    14521 acgccgagca gcacggcgcg ctggaaattg atgaaaggct ggctcatctg gtcggcattg
    14581 acccacgcgc cgccggaaag cacgaaggtt gcgccgcgat agatggtcag cgtgccgagc
    14641 gtcaccacga tggacgggat attgagacgc cagaccagca ggccgttgat ggcgccaagc
    14701 gccagcccga ccgccagcgc cacgacgatc agcaccggga taggaatggc cggatgcgtg
    14761 gcgttcagca tcgccaccac catgccggtg aagcacagat tggcggccat cgacaggtcg
    14821 atggcgcgcg tcagaatgac cgtcatctgg ccaagcgcca ggatcat
//
LOCUS       DASYCS010000427         5361 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_850248,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000427 DASYCS010000000
VERSION     DASYCS010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 5361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5361
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_850248"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /locus_tag="VGM46_13095"
     CDS             <1..981
                     /locus_tag="VGM46_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010914983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HEY4193576.1"
                     /translation="TTARQIYAFAVAKARGWDGPADRLIAHGIAFMAKKGRTDRGGWV
                     RTLNCDGSVADATEDAYDHACILLALAHAHMGGNPDALQLGQQTFSFLDEHLEDVRMT
                     GFLESSDGGGERRSNPHMHLLEAFLAWHDATGDRTYLRRAARIIDLFRSHFFDVESWT
                     LGEFFDDEWKPAPGERGAWTEPGHHFEWASLLVDFAARSGQNDLNGFARKLYASAIAN
                     GLNRSTGLAYGAVSRNGIPLDGLSRSWPQTEAVKAAIALDQTGGPDLKPEIEERVGRL
                     FRWHIDPAPLGLWIDRIDERGHSRASEVPASIFYHLVCALTQYLDGTGKE"
     gene            complement(992..2455)
                     /gene="betB"
                     /locus_tag="VGM46_13100"
     CDS             complement(992..2455)
                     /gene="betB"
                     /locus_tag="VGM46_13100"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010914984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HEY4193577.1"
                     /translation="MRAQPTASHYINGRFVDDEQGALMPVIYPASGETIATLHSATPN
                     IVELAIEAARAAQPAWARLKPVERGRILRRAADILRARNADLARLETLNTGKALQETL
                     VADAPSAADCLEYFGGAIPAFNGEAVDLGGPFAYTRREALGVCVGIGAWNYPIQIAGW
                     KSAPALAMGNAMVFKPSENTPLSALALAEIYTEAGLPDGLFNVVQGYGDVGAALVEHE
                     AVAKVSVTGSVPTGRRVLSVAGSKMKHATMELGGKSPLIVFDDADIENAIGGAMLGNF
                     YSTGQICSNGTRVFVQEGIHDRFVERLVERTKKIRIGDPLDEETQMGPMVSKAQHEKV
                     AGYFEIGRQDGATLACGGNVPSLQGFEGGFFIEPTVFTGVTDTMRIAREEIFGPVMSV
                     LKFEDEDEVIERANATEFGLAAGVFTRDLARAHRVVAELQAGTCWINTYNLTPVEIPF
                     GGAKQSGIGRENSLAALALYSQLKSVYVETGDVASPY"
     gene            complement(2523..4172)
                     /gene="betA"
                     /locus_tag="VGM46_13105"
     CDS             complement(2523..4172)
                     /gene="betA"
                     /locus_tag="VGM46_13105"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012092477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HEY4193578.1"
                     /translation="MEADFVIIGSGSAGSAMAYRLSEDGRHSVIVIEFGGTDMGPLIQ
                     MPSALSIPLNMPHYDWGFASEPEPHLGGRVLATPRGKVIGGSSSINGMVYVRGHAHDY
                     DHWAGQGATGWAFADVLPYFKRMEDSVGGEEGWRGKGGPLHVQRGLRRNPLYQAFIEA
                     GRQAGFELTDDYNGSKQEGFGPMEQTIFGGRRWSAANAYLRPALKRQNVRLVKGFARR
                     VVIENQRATGVEIEAHRTIQIVKARREVIVAASSINSPKILMLSGIGPAKHLKANGID
                     VIADRAGVGQNLQDHLELYIQQESTQPITLNSVLNPFSKALIGARWLLFKNGLGATNH
                     FEAAAFVRSQAGVDYPDIQYHFIPAAVRYDGKAAAKAHGFQAHVGPMRSKSRGAVTLR
                     SPDPLAKPEIRFNYMSHPDDWADFRRCIRLTREIFGQAAFDPFRGEEISPGRAIRNDD
                     ELDGFIRQHVESAYHPCGTCKMGRADDMSSVVDPECRVIGVDGLRVADSSIFPRVTNG
                     NLNGPSIMTGEKASDHILGRAPLSPSNQEPWINPRWQVSDR"
     gene            complement(4181..4819)
                     /locus_tag="VGM46_13110"
     CDS             complement(4181..4819)
                     /locus_tag="VGM46_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEY4193579.1"
                     /translation="MITIWGRATSSNVQTVMWAVAELGLTHERIDAGGPFGGLDTPEF
                     AAMNPNRLIPVLRDGGEYLWESASIVRYLGARYGGEQFWPSDPLRRARLDQWSDWTKT
                     TFVPAFISRVFWPIMGKRPGDRDEAAIAAGAANLAGVLGVFDRRVSEDAYLGGDEPCF
                     ADIVAGTLLYRYFTLEFERAALPNLSAYYERLTRRPAYVRHVMVSYDSLRAK"
     gene            complement(4821..>5361)
                     /locus_tag="VGM46_13115"
     CDS             complement(4821..>5361)
                     /locus_tag="VGM46_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010914987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase/phosphatase domain-containing protein"
                     /protein_id="HEY4193580.1"
                     /translation="GLIVQPTSNLDITPTLCDLAGIDIAAIMPWTDGQSLKPAIDGHV
                     RTGPVLMEYAAEGSDAPLVAIRDGRYKFIHCEIDPPQLFDLESDPHELTNLAVDPANA
                     GLVAAFMAKVRARWDMAAFDAAVRASQASRWVVYPALRNGAYYPWEFQPLQKASERYM
                     RNHMDLNVLEEQKRFPRGE"
BASE COUNT          942 a         1721 c         1720 g          978 t
ORIGIN      
        1 accaccgcac ggcagattta cgccttcgcg gtggccaagg cgcgcggctg ggacggcccg
       61 gccgatcggc tgatcgccca tggcatcgcc ttcatggcca agaagggccg gaccgatcgc
      121 ggcggctggg tgcgaacgct caactgcgac ggcagcgtcg ccgacgcgac cgaagacgcc
      181 tacgatcacg cctgcattct cctggcgctg gcgcatgcgc atatgggcgg caatccggac
      241 gcgctgcaac ttggccagca gaccttctcg ttcctggacg agcacctgga agatgtccgc
      301 atgaccggct ttctcgaaag ctcggacggt ggcggcgaga ggcgctccaa cccgcacatg
      361 catcttctgg aagcgtttct ggcttggcac gatgcgaccg gcgaccgcac ctatctgcgc
      421 cgggcagccc gcatcatcga cctgtttcgc agccatttct tcgacgtgga aagctggacg
      481 ctcggcgaat ttttcgacga tgagtggaag ccggcgccgg gcgagcgagg cgcatggacc
      541 gagcctggcc accatttcga atgggcctcg ctgctggtcg atttcgccgc ccgcagcggc
      601 cagaacgacc tcaacggctt cgcccgcaaa ctatatgcct ccgcgatagc caatggcctg
      661 aaccgctcca ccggccttgc ctatggcgcg gtgtcgcgca acggcatacc gctcgatggc
      721 ctgtcgcgca gctggccgca gacggaagcg gtcaaggcgg ccatcgcgct cgaccagacc
      781 ggcgggccgg atctcaagcc ggagatcgag gagcgtgtcg gccgcctgtt tcgctggcat
      841 atcgatcccg cgccgctcgg cctgtggatc gaccgcatcg atgagcgcgg ccactcgcgc
      901 gccagcgaag ttcccgccag catcttttac cacctcgtct gcgcgctgac gcaatatctc
      961 gacggcacgg gcaaggaata gaagcggtcc ctcaataggg gctggcgacg tcgccggtct
     1021 cgacatagac cgacttcaac tgcgaataga gcgccaatgc ggccagcgaa ttctcgcggc
     1081 cgatgccgga ttgcttggcg ccgccgaacg gaatttccac cggcgtcaaa ttgtaggtgt
     1141 tgatccagca ggtgccggcc tgcaactcgg cgacgacgcg gtgggcgcgt gcgagatcgc
     1201 gcgtgaacac gcctgccgcc aggccgaact cggtggcgtt ggcgcgctcg atcacctcgt
     1261 cctcgtcctc gaatttcaaa acgctcatca ccgggccgaa tatttcctcg cgcgcgatcc
     1321 gcatggtgtc ggtgacgccg gtaaagacgg tcggctcgat gaagaagccg ccttcaaagc
     1381 cctgcaagga gggcacgttg ccgccgcatg ccagtgtcgc accgtcctgc ctgccgattt
     1441 cgaaatagcc ggcgaccttc tcgtgctgcg ccttgctcac catcggtccc atctgcgtct
     1501 cttcgtcgag cgggtcgccg atgcggattt tcttggtgcg ctcgaccagc cgctcgacaa
     1561 aacggtcgtg aatgccctct tgcacgaaga cgcgggtgcc gttcgaacag atttgcccgg
     1621 tcgagtagaa attgccgagc atcgcgccgc cgatggcgtt ttcgatgtcg gcgtcgtcaa
     1681 acacgatcag cggcgacttg ccgccgagtt ccatggtcgc gtgcttcatc ttcgagccgg
     1741 caaccgacag gacccgccgc ccggtcggca cggagccggt caccgaaacc ttggcgacag
     1801 cctcgtgctc gaccagggct gcgcccacgt cgccatagcc ctggacgacg ttgaacagcc
     1861 cgtccggcag gccagcctcg gtatagattt cagccagcgc cagtgcggaa agcggcgtgt
     1921 tttccgaagg cttgaacacc atggcgttgc ccatggccag cgccggcgcg gatttccagc
     1981 cggcgatctg gatcgggtag ttccaggcgc cgatgccgac gcatacgccg agcgcctcgc
     2041 ggcgcgtata ggcgaagggg ccgcccagat cgaccgcctc gccgttgaag gcggggatcg
     2101 cgccgccgaa atattccagg cagtcggccg ccgatggcgc gtccgcgacc agcgtttcct
     2161 gaagcgcctt gccggtgttc agcgtctcaa gccttgcaag gtcggcattg cgcgcccgca
     2221 atatgtcggc ggcccggcgc aggatgcgcc cgcgctcgac tggcttcagc ctcgcccatg
     2281 ccggctgcgc ggcgcgcgcc gcctcgattg caagttccac gatgttgggc gttgccgaat
     2341 gcagcgtggc gatggtttcc ccgctcgccg gataaatgac gggcatgagc gcgccttgct
     2401 cgtcatcgac gaagcggcca ttgatatagt gcgaggccgt gggctgggcg cgcattgtgg
     2461 tctccaaatc actgtggttc tgcggggtgg ttttgcgggt cgtgcggccc gcccatcatg
     2521 ctctatctgt ccgacacctg ccagcgcgga ttgatccatg gttcctggtt ggaaggcgaa
     2581 agcggcgcgc ggccaagaat gtggtcggat gctttttcgc cggtcatgat cgacggcccg
     2641 ttcagatttc cgttggtgac gcgcgggaag atcgatgaat cggcaacacg caggccatcg
     2701 acgccgatga cccggcattc gggatcgacc acgctgctca tgtcgtccgc gcggcccatc
     2761 ttgcaggtgc cgcaggggtg ataggcgctt tcgacatgct ggcggatgaa cccgtccagt
     2821 tcgtcgtcgt ttcggatggc gcgtcccggc gagatttcct cgcctcggaa cgggtcgaac
     2881 gcggcttggc cgaaaatttc acgcgtaagc cggatgcagc gccgaaagtc ggcccagtcg
     2941 tcggggtgcg acatgtagtt gaagcggatt tcaggctttg ccagcggatc gggcgagcgc
     3001 agcgtcaccg cgccgcgcga tttcgagcgc atggggccga catgcgcctg gaagccgtgc
     3061 gccttggcag cagccttgcc gtcatagcgc acggccgccg ggatgaagtg atactggatg
     3121 tcgggatagt cgacgccggc ctgcgaccgc acgaaggcgg cggcctcgaa atgattggtg
     3181 gccccaagcc cgttcttgaa cagcagccag cgcgcgccga tcagtgcctt ggagaaagga
     3241 ttcagcacgg aattgagcgt gatcggctgc gtcgattcct gctggatata aagctccaga
     3301 tgatcttgca ggttctggcc gacgccggct cgatcggcga tcacatcgat gccgttcgcc
     3361 ttgaggtgct tggccggtcc gatgcccgaa agcatcagga ttttcggcga attgatcgac
     3421 gacgccgcaa cgatcacctc gcgccgcgcc ttgacgatct gaattgtccg gtgtgcttcg
     3481 atctcgacgc cggtggcacg ttgattctca atgaccacgc gccgggcgaa gcccttgacc
     3541 agacgaacgt tttgccgttt cagcgccggc cgcagatagg cgttcgccgc cgaccagcgg
     3601 cgcccgccga agatggtctg ctccatcggc ccaaaacctt cctgcttcga accattatag
     3661 tcgtcggtca gttcaaatcc ggcctgccgg cctgcttcga tgaaggcctg gtagagcgga
     3721 tttcggcgca ggccgcgctg cacatgcaaa gggccgccct tgccgcgcca gccttcctcg
     3781 ccgccgaccg aatcttccat gcgcttgaaa tagggaagca cgtcggcaaa ggcccagcct
     3841 gtagctcctt gcccggccca atggtcgtaa tcatgggcat ggccgcgcac atagaccatg
     3901 ccattgatgg acgaagagcc gccgatgacc ttgccgcggg gtgtcgccag cacccgtccg
     3961 cccagatgcg gttccggctc gctggcaaaa ccccaatcgt aatgcggcat gttgagcggg
     4021 atggacagcg ccgaaggcat ctggatcagc ggtcccatat cggtgccgcc gaactcgatg
     4081 acgatgaccg aatgcctgcc gtcttccgac aggcgataag ccatggccga cccggcggaa
     4141 ccggacccga tgatgacgaa atcggcttcc agggcagcgc tcatttggcc ctcaggctgt
     4201 cgtaggaaac catgacgtga cgcacatagg cgggacgcct cgtcagccgc tcgtaatagg
     4261 cgctaagatt gggcaatgcg gcgcgctcga attccagcgt gaaatagcgg taaagcagcg
     4321 tgccggcgac gatatcggca aagcacggct cgtcgccgcc aagataggcg tcttccgaaa
     4381 cccgccggtc gaaaacgcca agcacgccgg cgaggttggc ggcacctgcc gcaatggcgg
     4441 cctcgtcacg gtcgccgggc cgcttgccca taatcggcca gaataccctg cttatgaatg
     4501 ccggtacaaa ggtggtcttg gtccagtcgc tccattggtc tagcctggcg cgccttaagg
     4561 ggtcgcttgg ccagaactgt tcgccgccgt agcgcgcgcc gagatagcgc acgatcgagg
     4621 cgctctccca aagatattcg ccgccgtcgc gcaggaccgg tatcagccgg ttggggttca
     4681 tggcggcgaa ttccggcgtg tccagcccgc cgaaaggccc acccgcgtcg atgcgctcat
     4741 gcgtgaggcc aagttcggcg acggcccaca tcacggtctg cacattggac gaggtggctc
     4801 tgccccagat ggtgatcatc ttattcgccc ctcggaaaac gcttctgttc ttcgagcacg
     4861 ttgagatcca tgtggttgcg catgtagcgc tccgaggcct tttgcagcgg ctggaattcc
     4921 caggggtagt aagcgccgtt gcgcagcgcc ggatagacca cccagcggct tgcctggctg
     4981 gcgcgcacgg ccgcgtcgaa agctgccatg tcccagcgcg cgcgaacctt cgccatgaac
     5041 gcggcgacca gtccggcgtt ggcggggtcg acggcaaggt tggtcagttc atgcggatcg
     5101 gattcaagat caaacagttg cggcgggtcg atctcgcagt gaatgaactt gtagcgcccg
     5161 tcgcggatcg ccaccagcgg cgcgtcggac ccctccgcgg catattccat cagcaccggc
     5221 ccggtgcgga catggccgtc gatggcgggc ttcagcgact ggccgtccgt ccacggcatg
     5281 atggctgcga tgtcgatgcc ggcgaggtcg cacagcgtcg gcgtgatatc gagattggag
     5341 gtcggctgca cgatgaggcc g
//
LOCUS       DASYCS010000428         1527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3024931,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000428 DASYCS010000000
VERSION     DASYCS010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3024931"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VGM46_13120"
     CDS             <1..252
                     /locus_tag="VGM46_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013851001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY4193581.1"
                     /translation="MTHVLTKELRGRNITVNSVAPGPTATNLFLNGKPQEVVDRLAKL
                     APLERLGQPEDIAAAVAFLAGPDGAWINGQVLRANGGII"
     gene            451..1350
                     /locus_tag="VGM46_13125"
     CDS             451..1350
                     /locus_tag="VGM46_13125"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017273200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY4193582.1"
                     /translation="MKKIVLITGASSGFGRMTAEALARAGHTVYASMRDIAGRNAANV
                     AQMAAFSSQNGVDLRSVELDVQSQPSVDAAVAQVIADSGRIDVLIHNAGHMVFGPAEA
                     FTPEQYAQLYDVNVLSTQRVNRAALPHMRARKEGLLVWVSSSSSAGGTPPYLAPYFGA
                     KAAMDAIAVQYARELSRWGIETSIIVPGAFTGGTNHFEHSGVPADHARAAEYDNGPYA
                     GFGEQVQKAFAKIVPPDADAGSVAEAIVEVVDKPFGKRPFRVHIDPTQDGADVGFTVL
                     DRVRAEMLHRVGLSDLLTPKAAA"
BASE COUNT          303 a          543 c          451 g          230 t
ORIGIN      
        1 atgacgcatg tgctgaccaa ggaactgcgc ggccgcaaca tcacggtcaa cagcgtggcg
       61 cccggcccca ccgcgaccaa ccttttcctg aacggcaagc cgcaggaagt ggtcgaccgc
      121 ctggccaagc tggccccgct cgaacgcctg ggccagcccg aagacatcgc cgcggccgtg
      181 gctttcctgg ccggccccga cggcgcatgg atcaacggcc aggtgctgcg cgccaacggc
      241 ggaatcatct gaagaccggc aagtacacaa ccgcctgcca cgatccgaat acgaagacct
      301 gcatctgagg acccgtattc gaaaaccagc gtttcgaaga cctgcgttcg aagacccgta
      361 ttcgaacccg cttccgcaat cccttatccg acgcgccatg cgagccggat accccacccc
      421 ggatcagatc gtcatccaag gagtccagcc atgaaaaaga tcgtcctcat caccggcgct
      481 tccagcggct tcggccgaat gactgcagag gccctggcca gggccggcca caccgtctac
      541 gcatccatgc gcgacatcgc gggccgcaac gccgccaacg tcgcgcagat ggccgcgttt
      601 tccagccaga acggcgtcga cctgcgcagc gtcgaactcg acgtgcaatc gcagccctcc
      661 gtggatgccg ccgtcgccca ggtcattgcc gactccggcc gcatcgatgt gctgatacac
      721 aatgccggcc acatggtctt cggtcccgcc gaggccttca ctcccgagca atacgcccag
      781 ctctacgacg tgaatgtcct tagcacccag cgcgtgaacc gggctgcgct gccgcacatg
      841 cgcgcgcgca aggaaggctt gttggtgtgg gtatcgagca gcagctcggc aggcggcacg
      901 ccgccctacc tggctcccta tttcggcgcg aaggccgcca tggatgcaat cgccgtgcaa
      961 tatgcacgcg agctgtcgcg ctggggcatc gagacgtcca tcatcgtgcc gggcgcgttc
     1021 accggcggca ccaatcactt cgagcactcc ggggtccccg ccgaccatgc ccgcgccgcg
     1081 gaatacgaca atggtcccta tgccggtttc ggcgaacagg tgcagaaggc cttcgcgaaa
     1141 atcgtgcctc cggatgccga cgccggcagc gtggccgagg ccatcgtcga ggtcgtcgac
     1201 aaacccttcg gcaagcgccc cttccgcgtg cacatcgacc ccacccagga cggcgcggac
     1261 gtcggcttca cggtgctcga ccgcgtacgc gccgagatgc tgcaccgggt cggcctgtcc
     1321 gacctgctca cgccgaaagc cgcggcataa cgggtaccgg cggagtacgg gcggagtacc
     1381 ggcgaaatcg gagccatggc cgccgcggcg gccatggccg gcattcaaaa aggcatcgga
     1441 gcactgtcat cgaactttcc gtcacctgaa aagcgtgacg caatcgtggc cgatgactgg
     1501 gcatcatcca aaagccacca tcaaaca
//
LOCUS       DASYCS010000429         2569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_5052932,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000429 DASYCS010000000
VERSION     DASYCS010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5052932"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VGM46_13130"
     CDS             <1..211
                     /locus_tag="VGM46_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002964699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEY4193583.1"
                     /translation="LRKGTTLTKEMFLNHLYGGMDEPELKIIDVFICKLRKKLDAASG
                     GQNYIETVWGRGYVLREPEDVRESA"
     gene            complement(312..680)
                     /locus_tag="VGM46_13135"
     CDS             complement(312..680)
                     /locus_tag="VGM46_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY4193584.1"
                     /translation="MKRCMFVDGSSVIRKVAKRILGGPDMIVIEASNCAEALAMCGAE
                     MPDILVIDAALEEMRTGDFIRSVRSLEAPIAPQILVSLVEVDIGAIMRAKRAGAHGYL
                     LKPFNRAQLIERFRTLDIAA"
     gene            complement(816..1472)
                     /locus_tag="VGM46_13140"
     CDS             complement(816..1472)
                     /locus_tag="VGM46_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008598600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphotransferase family protein"
                     /protein_id="HEY4193585.1"
                     /translation="MADLFTLSAPDLAALLCSRVCHDIISPVGAINNGLELLDEGGAD
                     EDAMNLIRQSARNASARLQFARIAFGAAGSAGMLIDTGDAESVALAFLKNEKPELTWT
                     GTRALLPKNKVKLLLNLILVANAAIPRGGRLSVALEDTETLPRFTLSASGPMLRVPPK
                     FLELHSGNQPEDPIDAHSVQPYYTLLLAREAGMTISIHATAEEIVLTAASPRQETAAE
                     "
     gene            1847..2380
                     /locus_tag="VGM46_13145"
     CDS             1847..2380
                     /locus_tag="VGM46_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006338664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EipA family protein"
                     /protein_id="HEY4193586.1"
                     /translation="MLAFAGFYGLSAPASQAQEYTAQEIVDSGHKFFGATSGGLASVV
                     EKIFASYGLPNGYLLGEEGSGAIIGGLTYGEGTLYTKNAGDHKAFWQGPSLGWDFGGE
                     GSRVMMLVYNLDDVSALYNRFGGVAGSAYVVAGVGFNVLKNNNMLLVPIRTGVGARLG
                     VNLGYLKLTSKPTWNPL"
BASE COUNT          518 a          701 c          804 g          546 t
ORIGIN      
        1 gctgcgcaag ggcacgacgc ttaccaagga aatgttcctc aaccacctct atggcgggat
       61 ggacgagccg gaactgaaga tcatcgacgt ttttatctgc aagctgcgca agaagctcga
      121 cgctgcttcc ggcggccaga attacatcga aacggtgtgg ggccgcggct atgtgctgcg
      181 cgagccagaa gatgtccgcg aaagcgcctg aaagttcgaa attccgttac agaaaagccc
      241 gggtcagccg ggcttttttt gtttgaggca gggttcgcct cgaatgtgtc tcgaaccgct
      301 gcggcaaaaa gttaagccgc gatatccagc gtgcgaaagc gttcgatcaa ttgcgcgcgg
      361 ttgaaaggct tcagcaaata gccgtgcgcg ccggcgcgtt tggcgcgcat gatggcgcca
      421 atatcgactt cgaccagtga aacgagaatt tggggggcga tgggagcttc caacgatcgc
      481 acgctgcgga tgaaatctcc ggtgcgcatt tcttcaaggg cggcgtctat cacgaggatg
      541 tccggcattt ccgcgccgca catcgccaac gcctcggcac agttcgaagc ctcgatcaca
      601 atcatgtcgg gtccgcccag gattcgcttg gcgacttttc tgatgacgct tgagccatcg
      661 acgaacatgc agcgtttcat gcctcacccc ccggatgcac gcgaacggat cgcggtgcgt
      721 ctggccgcat gtgtaaacgc tccaggtaaa aaagcggaaa aggcattcgg taaatttttc
      781 ccgaatgcac gctttgcggg aaatgcggcg cgagactatt cggccgctgt ttcctgtcgg
      841 ggcgaggcgg cggtcaggac gatctcttcc gccgtggcgt ggatggagat ggtcatgccg
      901 gcctcgcgcg ccagaagcag cgtgtaataa ggctgcaccg aatgcgcgtc gatggggtct
      961 tccggttggt tgcccgaatg aagctcaagg aacttcggcg gcacgcgcaa catcgggccg
     1021 ctggccgaaa gcgtgaagcg cggcagcgtt tcggtatctt ccaaggccac cgaaagcctg
     1081 ccgccgcgcg ggatggccgc attggcgacc aaaatgaggt tgagcagcag cttgaccttg
     1141 ttcttgggca gcaatgcgcg ggttccggtc catgtcagtt ccggcttttc gttcttcaag
     1201 aaagccaggg cgaccgattc ggcatcgccg gtgtcgatca gcattccggc ggaaccggcg
     1261 gcgccaaagg cgatgcgggc aaattgcagg cgggccgaag cgttgcgcgc gctctggcgg
     1321 atgaggttca tggcgtcttc gtcggcgccg ccctcgtcga gaagttcaag gccattattg
     1381 atggcgccga ctggcgaaat gatgtcgtgg cagacgcggc tgcacagcag cgccgccaga
     1441 tcgggagcgg atagggtgaa aaggtcagcc atgaaaaaat ccccgtgaag gccacaaacg
     1501 acaagacgat gttttgaccg agcatcgcct gtccgaatca ggttttgtga accttgtcgg
     1561 gtatagcgcg ggcaaaagct gcgagaaatc caaaatcgag cttacagacg atagtatatt
     1621 gcggccgagc gtcctcgggc cgtgccgtat ggccttcgtg ccgccatgtt catgtgcgag
     1681 cttaatggtt gaaaaacgat tacggcataa tttgcccgca tgaacacccg aagcggccgc
     1741 taaagagcca gtaagggcga ggcgtcggcg aaagcgctcc ctgacggaga ttggaagcat
     1801 gttttcccga ttgtatgacc ggagtttttt ccggattttt gccgtgatgc ttgcctttgc
     1861 gggcttttat ggtctttccg cccctgcctc gcaggcgcag gaatataccg cgcaggagat
     1921 cgtcgattcc ggccacaagt tttttggcgc tacctccggc ggcctcgcca gtgtcgttga
     1981 gaagattttc gcgtcctacg gcctgccgaa cggctatctt ctgggcgagg aaggctcggg
     2041 cgccattatt ggcgggctga cctatggcga gggcacgctc tacacgaaaa acgccggcga
     2101 ccataaagcg ttctggcagg gtccttcgct cggctgggat ttcggcggcg aaggctcgcg
     2161 ggtgatgatg ctcgtctata atctggacga tgtcagcgcg ctttataacc gcttcggcgg
     2221 cgttgccggt tcggcctatg tcgtggccgg cgtcggcttc aacgtgctca agaacaacaa
     2281 tatgctgctg gtgcctatcc gtaccggtgt cggggccagg cttggcgtca atctcggcta
     2341 tctcaagctt accagcaagc cgacctggaa tcctttatga ggttcggccg ctagcagtac
     2401 gtccaatggg gtgcgcttgc cggcattatc gcctcgatcg agcgttggat tcgaccggaa
     2461 accgataaac ggttccggtc gaataatgct tggaatacgc atctgcctgc cggccgttgg
     2521 cctgcactgg attttgcgtg cgtgaacgtg cgacaattcg taggcggtt
//
LOCUS       DASYCS010000430         8524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1726732,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000430 DASYCS010000000
VERSION     DASYCS010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 8524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8524
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1726732"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..1570
                     /locus_tag="VGM46_13150"
     CDS             113..1570
                     /locus_tag="VGM46_13150"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY4193587.1"
                     /translation="MLQKTSHFMRQANLINGEWVQADSGATIDVTNPATGLKIGTVPK
                     SGKAETRRAIEAAEAAFKSFRKTSALDRSKMLRKLHDAIMDNQDALAELLTLEQGKSL
                     TESRGEIASSAAYVLWFAEEARRAYGDVVPSPWADRRVLVTREPVGVIAAITPWNFPS
                     SMLSRKIGPAIAAGCTAVVKPASQTPYSGLAWGALCEEAGIPKGVVNIITGAAGEIGD
                     EICANPIVKKITFTGSTEVGKMLIEKSASTVKKVSMELGGNAPFLVFDDADLDRAVAG
                     AIAAKYRNSGQTCVCTNRFFVQAGIHDEFVARFAKASNELKVGSGLDEGVQQGPLIDG
                     KAVEKVEEFIADATAKGGKVVSGGKRHKLGGSFFEPTVISGATSDMMFMKDEIFGPVA
                     PIFKFETEDEAVALANDTEFGLASYFYTGDLGRAFRVMEGLKYGMVGINEGIITTPEA
                     PFGGVKESGLGREGGHQGIDDYLDIKYVCIGGLGL"
     gene            1594..2613
                     /locus_tag="VGM46_13155"
     CDS             1594..2613
                     /locus_tag="VGM46_13155"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019858637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HEY4193588.1"
                     /translation="MKDGEVFGTTQGGDIIRRFTIRGGGLSANIIGLGAIVQDLRIAG
                     HEAPLVLGYDRLESYESDTAYFGAAVGRYANRIRDGRFTLGGERHQTDRNALGKHTLH
                     GGSGGYAHRPWTVSLHGRDFVTLSLHDPAGEMGFPGALEITCTYRLKIPGTLAVEFSA
                     TTDEPTICNLTQHSYFNLDDGGASDILDHRMMLNAAAYLPVDGDTIPTGFVVPVDGTP
                     FDFRQARPLRMEAEGRQLPYDENFCLCACRGPLRQAAWVQGAASGLEMEVWTTEPGVQ
                     LYTGQFIAAPGPGLGGLGYRPYSGFCLEPQIWPDAPNRSYFPQAVLLPGERYHHVTEY
                     RFRLP"
     gene            2843..3289
                     /locus_tag="VGM46_13160"
     CDS             2843..3289
                     /locus_tag="VGM46_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006335395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HEY4193589.1"
                     /translation="MASLGRYILAALALAAVLCGIVVAVYLAGPGRGDAKSDLSRPKK
                     SAHGLFVVSLMPESGAIKTGVLQSWLLTLKTASGSPVEFAAIDISGGRPRQAHGLPTS
                     PQATDYLGQGRYRVEGLKFTVSGWWQLRLAISAAAGSDTVVFNVVL"
     gene            complement(3309..3758)
                     /locus_tag="VGM46_13165"
     CDS             complement(3309..3758)
                     /locus_tag="VGM46_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEY4193590.1"
                     /translation="MEDQIDRIDRNILSALSRHGRISMAELATKVGLSKTPVQARVKR
                     LEKDGYIRGYQAIVDRERMGEGHVAFVQVKLSDTRSAALDAFNRAVQAVGEIEQCHMM
                     ASSFDYLLKVRTQDIAAYRRVLGERISALPHVAQTSTFVAMETVKDR"
     gene            3920..7531
                     /gene="putA"
                     /locus_tag="VGM46_13170"
     CDS             3920..7531
                     /gene="putA"
                     /locus_tag="VGM46_13170"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HEY4193591.1"
                     /translation="MPSLDLIRQQIRANILPDEDEAVKRLAEAAHLSVEDRKAISARA
                     VKLVDAVRGSTDPRLMEVFLSAYGLSTKEGVALMCLAEALLRVPDAETMDDLIADKIA
                     PHDWSAHSGGSSSVFVNASTWALMLTGRVLDEGEGGIEGTLRSMVRRLGEPVIRKAVA
                     AAMREMGEQFVLGRSIAEAMKRGRSMTQKGYLYSFDMLGEAARTEADALRYLKAYADA
                     ISSLDTPSNGPDIRQNHGISVKLSALHPRYEAAQKATMLPVMTERLLSLALAARNARM
                     GLNVDAEEADRLDLSLDVIEQVLADPRLSEWDGFGVVVQAYGPRAAFVIDWLYALARK
                     HDRNIMVRLVKGAYWDTEIKRAQVLGLDGYPVFTRKANTDVSYVSCAKKLLGMTDFIY
                     PQFATHNAHTVAAILSMASDRHSFEFQRLHGMGEALHETVRKAEGTRCRIYAPVGAHS
                     DLLAYLVRRLLENGANSSFVHQLTDKDVEPREIARDPLDIVEKQGPARNPNIPAPLAI
                     FGGGRRNSKGWDLTDPLVLAALEEARAAFAGPECWRASPITPAAGNGPERKIINPAWL
                     DDTVGTVIEATATQVAAAIGIAADAQPAWARLPVTQRSAVLERAADLYEANAVELFAL
                     ATREAGKSLADGVSEVREAVDFLRYYARQAGDAEQGTMARGVIACISPWNFPLAIFTG
                     QVAAALVTGNAVIAKPAEQTPLMAYRAVELLREAGVPEGIVQLLPGDGPSVGGPLTAD
                     PRIAGVCFTGSTEVAKLIERQLAETAAPDAMLIAETGGLNAMIVDSTALPEQAVRDIL
                     ASAFQSAGQRCSALRVLYVQKDVEKKMLEMLKGAMEALKVGDPWQISTDVGPVIDDDA
                     KTSIEEYCDKMFLDGRLVAGLAAPEGGRFVGPQIFKVSGIEEMEREVFGPVLHVATFD
                     ADKIDSVISAINRKGYGLTFGLHTRIEGRVQHFVDGIHAGNIYVNRNQIGAVVGSQPF
                     GGEGLSGTGPKAGGPHYLRRFRKGVEAGTHLGEGRKVTATELADNLPDQAQAGWSTRS
                     DRVAVLRKHLRGKGAAAIAAAASLDFGQVDLPGPTGEANTLSLVPRGRVLCLGPDADT
                     LLAQVIQALAAGNAVLAVAPGAPAALAALTGKGLPLGVIDGRADPVEARSLRVDVVAF
                     SGTPEATRTVRKVVADRNGPIVPLVTEVLYPAAYAHERGVCVDTTAAGGNASLLAAA"
     gene            complement(7556..8362)
                     /locus_tag="VGM46_13175"
     CDS             complement(7556..8362)
                     /locus_tag="VGM46_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006334780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEY4193592.1"
                     /translation="MSNPGTIRAGMGGWTFEPWDTSFYPEKLAKTKQLHYATRQVPTI
                     EVNGTYYSSFKEPTFVKWANESPDGFVFSLKGNRFVTNRRVLGEAGESMTRFLESGVA
                     ALGEKLGPILWQFMPTKKFDPDDFEAFLKLLPEKQDGVKLRHALEVRNDSFVVPEFAA
                     LARKYKVAIVYADHAKYPAIADITGDFVYARLQTGKDDNPDCYEPKRLDEWAERAKVW
                     AQGKAPADLPRADPSSEAPVKPRDVFMYFITEGKVRAPFGAMALMKRVDG"
BASE COUNT         1535 a         2563 c         2794 g         1632 t
ORIGIN      
        1 gccttcccgt gcgttatgaa cgaaggcact tcggccgccc ggcctttgcc gggccgcccg
       61 gtcaaacgaa ttgcaaacag gcgcttgagc gcaccagaac agggtgaaag acatgctcca
      121 gaaaacaagc catttcatgc ggcaggccaa tctcatcaat ggcgagtggg tgcaggccga
      181 ttcgggtgcg accatcgacg tgaccaatcc ggcgaccggc ctcaagatcg gcacggtgcc
      241 gaaatccggc aaggccgaga cccgccgcgc catcgaggcg gcagaggccg ccttcaagtc
      301 gttccgcaag acttcggcgc tcgatcgttc caagatgctg cgcaagctgc atgacgccat
      361 catggacaac caggacgcgc tggccgaact gctgacgctg gagcaaggca agtcgcttac
      421 cgaatcgcgc ggcgagatag cttcttcggc ggcctatgtc ttgtggtttg cagaggaagc
      481 gcgccgcgct tatggcgatg tggtgccttc gccctgggct gatcggcgcg ttctggttac
      541 ccgcgagcct gttggcgtga ttgccgccat cacgccttgg aacttcccgt catcgatgct
      601 gtcgcgcaag ataggcccgg ccatcgcagc gggctgcacg gcggtcgtca agccggcgtc
      661 gcagacgcct tattcgggcc ttgcctgggg cgcgctgtgc gaggaagccg gcattcccaa
      721 aggcgtggtc aatatcatca ctggcgcagc cggggagata ggcgacgaga tctgcgccaa
      781 tccgatcgtc aagaagatca ctttcactgg ttcgacggag gtcggcaaga tgctgatcga
      841 aaagtcggcc tcgaccgtca agaaggtgtc gatggaactg ggcggcaacg cgccgttcct
      901 ggtgttcgac gatgccgatc tcgaccgcgc ggttgctggc gccatagccg cgaaataccg
      961 caactccggc cagacttgcg tttgcaccaa ccggttcttc gtgcaggccg gcatccatga
     1021 cgagttcgtc gccaggtttg ccaaggccag caacgagttg aaggtaggct cggggctgga
     1081 cgaaggcgta cagcaaggcc cgctcatcga tgggaaagca gtcgagaagg tcgaggaatt
     1141 catcgccgac gcaaccgcca agggcggcaa ggtggtcagc ggcggcaagc gccacaagct
     1201 tggcggttcg ttcttcgaac cgaccgtcat ttccggcgcg acgagcgaca tgatgttcat
     1261 gaaggacgaa atcttcggac cggtcgcgcc gatcttcaag ttcgaaaccg aggacgaggc
     1321 ggtcgcgctc gccaacgaca ccgagttcgg ccttgcctcc tacttctaca ccggcgacct
     1381 cggccgtgcg ttccgggtca tggaaggcct gaaatacggc atggtcggca tcaatgaagg
     1441 catcatcacc acgccggaag cgccgtttgg cggggtcaag gaatccggcc ttggccgcga
     1501 gggcgggcat cagggcatcg acgattacct cgacatcaaa tatgtctgca tcggcggcct
     1561 tggtctttag gcgggaaatc ggtaaaatcg ggcatgaaag acggcgaggt cttcggaacg
     1621 acacagggcg gcgacattat ccgccgcttc acgatacggg gcggcgggct tagcgcgaat
     1681 attatcggct taggcgcaat cgtccaggat ttgcggatcg ccggacacga ggcgccgctg
     1741 gtgctcggct atgaccggct ggagagctac gaaagcgaca ccgcctattt cggggcggcg
     1801 gttggacgtt acgccaaccg catccgcgac ggtcgcttca cgctgggcgg ggagcggcac
     1861 cagaccgacc ggaatgcgct gggcaagcat acgctgcatg gcgggtcggg tggttacgcg
     1921 catcggccat ggacggtttc gctgcatggc agggatttcg tcacgctgtc cctgcacgat
     1981 ccggccggcg agatgggttt tcccggcgcg ctggagatta cctgcaccta tcgcctgaaa
     2041 attcccggta cgctggcggt ggaattcagc gccaccaccg acgagccgac gatctgcaat
     2101 ctgacgcagc attcctattt caatctggat gatggcggcg ccagcgatat cctcgatcac
     2161 cggatgatgt tgaacgccgc cgcctacctg ccggtggacg gagatacgat cccaaccggc
     2221 ttcgtcgtcc cggttgacgg tacgccattc gacttccggc aggcgagacc cttgcggatg
     2281 gaggcggagg ggcggcaact gccttacgac gagaatttct gcctttgtgc ttgcaggggg
     2341 ccgctccggc aggccgcgtg ggtgcagggc gctgcttccg gcctcgaaat ggaggtgtgg
     2401 accacggaac ccggcgtcca gctttacacc ggccagttca tcgccgcccc cggacccggc
     2461 ctcggcggcc tgggctatcg cccatattcc ggcttttgcc tggaaccgca aatatggccg
     2521 gatgccccaa acaggtccta ttttccgcag gccgtgctgt tgcccggcga aagataccac
     2581 cacgtcaccg aatacaggtt ccgtctgcct tgagccaagc aggccgggtg gttgccgaca
     2641 ttagcggcgc gatcgccgtt ccggcgtggc gggaagcctg tgcagccttt ttggggccat
     2701 ggcgttttat tgccgccgcg ccatgcttat gtcacgcggc tttcttgtcg gcgaaataga
     2761 ccgaacttgt cggtgaaata tccgggaagg attgggtttg cggcgagccg gcgtggtatt
     2821 cgtccgggcg aaggaaacag gtttggcttc attagggcga tatattctgg cggctctcgc
     2881 cttggcggcg gtgctttgcg gcatcgtggt cgccgtctat ctggccgggc ctggccgcgg
     2941 cgacgccaaa tccgatctgt ccaggccgaa aaagtcggcg catggattgt tcgtcgtaag
     3001 cctcatgccg gaaagtggcg cgatcaagac gggtgtgctt cagtcctggc tgctaacgct
     3061 gaaaacggcg tccgggtcgc cggtggaatt cgcggccatc gacatttcag gtggcaggcc
     3121 gcgacaggcc cacggcctgc cgaccagccc gcaagcgacg gactatctcg gccagggccg
     3181 ctaccgcgtc gaagggttga agttcaccgt cagcgggtgg tggcagttgc ggctggccat
     3241 ttcagcggcg gcaggttcgg acaccgtggt cttcaacgtg gtgctgtagg cgaaacccaa
     3301 aggctgggct acctgtcctt gaccgtttcc atcgccacga atgtcgaggt ttgcgctaca
     3361 tggggcagcg cggagatgcg ctcgccaagc accctgcggt aggcggcaat atcctgggtg
     3421 cggaccttca gcagataatc gaagctggat gccatcatgt ggcattgctc gatctcgccg
     3481 acagcctgca cggcgcggtt gaatgcgtcg agcgcggcgg agcgcgtatc ggatagtttg
     3541 acctgcacga aggcgacatg gccttcgccc atccgctcgc ggtcgacgat ggcctggtag
     3601 ccgcgaatgt agccatcctt ctccagccgc tttacccgcg cctgaacagg cgtcttcgac
     3661 aggccgacct tcgtggccag ttcggccatg gaaatgcggc cgtgacggga aagcgccgag
     3721 agaatgttcc ggtcgatgcg atctatttga tcttccatgg tgactatttc gacctatata
     3781 taccataagc agctttcatc ttagttcaga tgggttgcct tgtcgatttc tttggaccga
     3841 tgaaattttc gccccgtgct agcttggcgc atccgctccg gctaacgtcg gagcgcgcca
     3901 gcgaagctat aggaacgcca tgccctcact cgatcttatc cgtcagcaga tacgcgccaa
     3961 tatccttccc gatgaggacg aggcggtgaa gcgcctggcc gaggcagcgc acttgtcggt
     4021 cgaggatcga aaggcaattt cggcgcgcgc ggtcaagctg gtggatgcgg tgcgcggttc
     4081 gaccgatccg aggctgatgg aagttttcct gtccgcctat gggctttcga ccaaggaggg
     4141 cgtcgcgctg atgtgcctgg ccgaagcgct gctgcgcgtg ccggacgctg aaaccatgga
     4201 cgacctgatc gccgacaaga ttgcgccgca tgactggtcg gcgcattcgg gcgggtccag
     4261 ctcggtcttc gtcaacgcct ctacctgggc gctgatgctg accggccgtg ttctggacga
     4321 gggcgaaggc ggcatcgagg gcacgctgcg ttcgatggtg cgccggctgg gcgaaccggt
     4381 catccgcaag gcggtggcgg ccgccatgcg cgaaatgggc gagcagttcg tgcttggccg
     4441 ctcgatcgcg gaggcgatga agcgcggccg ctcgatgacg cagaaaggct atctctattc
     4501 cttcgacatg ctgggcgagg cggcccgcac cgaggcggat gccctgcgct acctcaaggc
     4561 ctatgccgat gcgatttcct cgctcgacac gccttccaac gggccggata ttcgccagaa
     4621 tcacggtatt tcagtcaagc tttcggcgct gcacccacgc tatgaagcgg cgcaaaaggc
     4681 gacgatgctg ccggtgatga ccgagcggct gctttcgctg gcgctggcgg cgcgcaatgc
     4741 ccgcatgggg ctgaacgtcg acgccgaaga agccgacagg ctggacctgt cgctcgatgt
     4801 gatcgaacag gtgctggccg atccgcggct gtcggaatgg gacggtttcg gcgtcgtcgt
     4861 gcaggcctat ggcccgcgcg ccgccttcgt catcgattgg ctctacgcct tggcccggaa
     4921 gcacgaccgc aatatcatgg tgcggctggt gaagggcgct tattgggaca ccgaaatcaa
     4981 gcgcgcgcag gtgcttggcc tcgatggcta tccggtgttc acgcgcaagg ccaacaccga
     5041 tgtttcctat gtgtcatgcg caaaaaaact gctcggcatg acggacttca tctacccgca
     5101 attcgccacg cataacgcgc acacggtcgc tgccatattg tcgatggcca gcgaccggca
     5161 ttctttcgaa ttccagcggc tgcacggcat gggcgaggca ctgcacgaga ccgtgcgcaa
     5221 agccgaagga acacgttgcc gcatctatgc gccggtcggc gcgcattccg atctgctggc
     5281 ctatctggtg cggcgtctcc tggaaaacgg cgccaactcc tcctttgtcc accagcttac
     5341 ggacaaggat gtcgagccgc gagaaatcgc gcgcgatccg ctcgatatcg ttgaaaagca
     5401 ggggccggcg aggaacccga acattccggc tccattggct atcttcgggg gtggccggcg
     5461 caattccaag ggttgggatt tgaccgatcc gctggtgctt gcggcgctgg aagaagcgcg
     5521 cgctgccttt gccgggccgg aatgctggcg cgccagcccg attacgccgg cggcaggcaa
     5581 tggccctgaa cgcaagatca tcaacccggc atggctggat gacacggtcg gcacggtgat
     5641 cgaagcgacg gccacccaag ttgccgccgc catcggcatc gcggccgacg cgcaaccggc
     5701 atgggccagg ctgccggtaa cgcagcgcag cgccgtgctg gagcgcgccg ccgatcttta
     5761 tgaagccaat gccgtggaat tattcgcgct ggcgacgcgc gaagccggga aaagcctggc
     5821 cgacggcgtg tcggaagtgc gcgaggcggt cgacttcctg cgctattatg caagacaggc
     5881 cggcgatgcc gagcagggca ctatggcgcg cggggtgatc gcctgcattt cgccgtggaa
     5941 tttcccgctc gccatcttca ccggccaggt cgcggcggcg ctggtgacag gcaatgccgt
     6001 gattgccaag ccggctgagc aaacgccgct gatggcctat cgcgcggttg aactgttgcg
     6061 cgaagccggc gtgcccgaag gcatcgtcca actgctaccc ggcgatgggc cttcggttgg
     6121 cggcccgctg acggccgacc cccgcattgc cggcgtctgc ttcaccggct cgacggaggt
     6181 ggccaagctg atcgaaaggc aactggccga gacagccgcg ccggatgcga tgctgatcgc
     6241 cgagacgggc ggcctcaacg cgatgatcgt cgattccacc gccttgcccg aacaggcggt
     6301 gcgcgacatt ctggcgtcgg ccttccagag tgccggccag cgctgttccg cgttgcgcgt
     6361 gctctatgtc cagaaggacg tggaaaagaa aatgctggag atgctgaaag gcgcgatgga
     6421 agccttgaag gtaggcgatc cgtggcagat atcgaccgat gtcgggccgg tcatcgacga
     6481 tgacgccaaa acgtcgatcg aggagtattg cgacaagatg ttcctggacg gccggcttgt
     6541 cgccgggctt gccgcgccgg agggcgggcg tttcgttggc cctcagatat tcaaagtcag
     6601 cggcatcgag gagatggagc gcgaggtttt cgggccggtc ctgcatgtgg cgacgtttga
     6661 tgccgacaag atcgacagcg tgatttcggc catcaaccgc aagggttatg gcctgacttt
     6721 cggcctgcat acccgcatag aggggcgggt ccagcatttc gtcgatggca ttcacgccgg
     6781 caatatctac gtcaaccgca accagatcgg tgcggtcgtc ggctcgcagc cgtttggcgg
     6841 cgaggggctg tccggcaccg gtccgaaggc cggcggcccg cattatctgc ggcggtttcg
     6901 caaaggcgtg gaagccggca cgcatcttgg cgagggccgc aaggtgacgg ctacggaact
     6961 tgccgataat ctgcccgacc aggcgcaggc aggctggtcg acgcggtccg accgcgtcgc
     7021 cgttctgcgc aagcatctgc gcggcaaagg tgcggcggcc attgccgcgg ctgcaagcct
     7081 ggatttcggg caggtcgatc tgcccggccc gaccggcgag gcgaatacgc tgtcgctcgt
     7141 gccgcgcggg cgcgttcttt gccttgggcc ggatgccgac acgctgctgg cgcaggtcat
     7201 ccaggcgctt gcggcgggca atgccgtgct ggccgtcgcg cccggcgcgc cggcagcgct
     7261 tgcggcgctg actggcaagg ggctgccgct cggcgtcatc gacggtcgcg ccgatccggt
     7321 cgaggcgcgc tcgctgcgtg tcgatgtggt cgccttttcc ggcacgccgg aggcgacgag
     7381 gacggtgcgc aaggtggttg ctgaccgcaa tgggccaatc gtgccgctgg tgacggaagt
     7441 gctgtatccc gccgcttacg cgcatgaacg cggcgtctgc gtcgacacga cggcagccgg
     7501 cggcaatgca agcttgctgg ccgcggcata ggttcgaaaa gcgggttggc tgccgtcagc
     7561 cgtcaacccg cttcatcagc gccattgcac caaagggcgc gcgaaccttg ccttcggtga
     7621 tgaagtacat gaaaacgtcg cgcggcttga ccggcgcttc gcttgaagga tcggcgcggg
     7681 gcaggtcggc cggcgccttg ccctgcgccc agaccttcgc ccgctcggcc cattcgtcca
     7741 gccgcttcgg ctcataacaa tccgggttgt cgtccttgcc cgtctggagg cgcgcataga
     7801 cgaaatcgcc ggtgatatcg gcaatcgctg gatatttcgc gtggtcggca tagacgatgg
     7861 ccaccttgta cttgcgcgca agcgcggcaa attccggcac gacgaagctg tcattgcgca
     7921 cttcgagcgc atggcgcagc ttcacgccat cctgcttttc gggcagcagc ttcaggaagg
     7981 cctcgaaatc gtccgggtcg aatttcttgg tcggcatgaa ttgccatagg atggggccga
     8041 gtttttcgcc caatgcggcg acgccggatt ccaggaagcg cgtcatggat tcgccggcct
     8101 cgccaagcac gcggcgattt gtgacgaagc ggttgccttt cagcgagaaa acaaagccat
     8161 cgggagattc attcgcccat ttaacgaatg tcggctcctt gaagcttgag taataggttc
     8221 cgttgacttc gatggtcggc acctggcgcg tggcgtagtg aagctgtttc gtcttggcca
     8281 gcttttcagg atagaacgag gtgtcccacg gctcgaaggt ccagccgccc atgcctgctc
     8341 ggatagttcc tgggttgctc attgccgtcc tcccgatttt cgacaagcca tttcccggcg
     8401 gaccggtttc gccatgttca cgatcgtcca tcccggccgg taaggatgcg gtccccggtt
     8461 cgtttcgtga aagccatagg ttttataaat ggcgatgttt ctttccattt tcgaactggc
     8521 aaaa
//
LOCUS       DASYCS010000431         4213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1001753,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000431 DASYCS010000000
VERSION     DASYCS010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4213
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1001753"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VGM46_13180"
     CDS             complement(<1..240)
                     /locus_tag="VGM46_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011927218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEY4193593.1"
                     /translation="MKAQVLNRYDDSLTADQWVTLQDVPDPKIEKSTDVIVRIGGAGV
                     CRTDLHIIEGVWRPFMDPNANDLLPLIMGHENAGWV"
     gene            465..2324
                     /locus_tag="VGM46_13185"
     CDS             465..2324
                     /locus_tag="VGM46_13185"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HEY4193594.1"
                     /translation="MQHLSGMDRARAALERGDRFPAGALTPIVAESWQRCRDLGLDPH
                     ASPRARVIPFSDVRRRRDANAALRRLALAEMQVLHSQIAGSNFMIALGDAEGVVLDTI
                     SDQRFADSPAGRSIIPGSMWNEAERGTNALGLAAANRQPIAIYGREHYFSCHGSLSCM
                     ASPILNSRGETIGLLDASCSNEARQQHTHVLVRMAAAQIENGLIFQERSESFIFAFHP
                     RAEYLDTLSAGLIAVSRDGEVFSFNRPGIALLAGLPTEPGGHFNTLFEASFSAAMDGL
                     LQGGLIKIKDRAGSGLFMVCRQIGQRAAVAANRRPNRAPAVVADPLAPNFVCQDAALK
                     HAMSDMAAAVTLKMPVQITGETGTGKELMARHVHDLSRRKGEFVALNCGAVPEQLFIS
                     EIFGHERGAFTNARNEGAPGLARLADNGTLFLDEVADIPPVAQTALLRFLDSMEVRTI
                     GGEKTHKVDVQIVSATNCNLQEMVAARTFRADLLYRLNAFTVRLPALDARSDFALIVQ
                     HLVQKISPGTAITDAAIAKLSQRRWPGNIRELHAVLQRALVRQRMEYLDEGSFEDIAR
                     NDDESCEACKPHPLNRQRCREIRATYQLSGDNVSKTARLLGLSRTTIYKHVRL"
     gene            complement(2489..2956)
                     /locus_tag="VGM46_13190"
     CDS             complement(2489..2956)
                     /locus_tag="VGM46_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003577183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HEY4193595.1"
                     /translation="MILPVDTAPQIGDRITDKSAPPEDGAIRNWIGPEAFERWGELRN
                     WIDEFYPGVFAPDWLYGGKNRGWSLRYKKTKAFTTLIPEYRRFSAVVVMGGAEREKFE
                     ERRYAWRSPLVKLYDEAKMYIDGKWLTLAVSSADDFDDVTGLLTMKRPPKPRD"
     gene            complement(3303..3965)
                     /locus_tag="VGM46_13195"
     CDS             complement(3303..3965)
                     /locus_tag="VGM46_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003577184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEY4193596.1"
                     /translation="MNILHIDVSPRQISHSRGLSAAIVGKLVEAAPGATITRRDLGSE
                     PLPHTPALYAAALASPTTLANPPEGSLDGSEALIGEIEAADAIVIGTPMYNFSIPSAL
                     KAWIDQVLRVGRTMQSTPNGKVGMLRDRPVFIAVSSGGVFGGERASQPDFVTPYLTLA
                     LNSIGLKSLQFLSIQATAFIDDDNAPKARQKALDTLDQTTVQQIQSAHRQGVDERVHA
                     QA"
     gene            complement(4081..>4213)
                     /locus_tag="VGM46_13200"
     CDS             complement(4081..>4213)
                     /locus_tag="VGM46_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018095196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY4193597.1"
                     /translation="VGYTDANIIEIVALVAMYSLTNFFNNVFDHDKDFPVVTPAGSI"
BASE COUNT          749 a         1275 c         1351 g          838 t
ORIGIN      
        1 tacccagccg gcgttctcat gccccatgat aagcggcagc aggtcgttgg cattggggtc
       61 catgaacggg cgccacacgc cttcgatgat gtgcaggtcg gtgcggcaca cgccggcgcc
      121 gccgatgcgc acgatcacat cggtggactt ctcgatcttc gggtcgggta catcctgcaa
      181 cgtgacccac tggtcggccg tgaggctgtc gtcatacctg ttcagtactt gagctttcat
      241 gcttatctcc ctgggcgccg tcatgagccc gtcagtcggg atgaaggaag caatagccgt
      301 gccagatggt gaagcttatt ggaaacaatg gcttgaccgc atccggcaat ggcccgtccg
      361 tacagcggct ggacatttga ccgaccgggt tgttcagttt ctggttattg cagtttcgac
      421 gtttgcgccc atactgcgcc cgctaatata tttcgggagg aatgatgcag catctgtctg
      481 ggatggaccg ggcgcgcgcc gcacttgagc ggggcgaccg ctttcccgcg ggagcgctta
      541 cgccgatcgt cgcggagagc tggcagcgct gccgcgacct cggcctcgac ccgcatgcca
      601 gtccgcgcgc cagggtcata cctttcagcg atgtgcgccg gcggcgcgat gcgaacgccg
      661 ccttgcgccg gctggctctg gcggagatgc aggttctgca ttcgcagatc gcggggtcga
      721 atttcatgat cgcgcttggc gatgcggagg gcgtggttct cgatacgatc agcgaccagc
      781 gctttgccga cagcccggcg ggccgctcga tcattcctgg cagcatgtgg aacgaagccg
      841 aacgcggcac caatgcgctc ggccttgccg cggccaaccg ccagcccatc gccatctatg
      901 ggcgcgagca ttatttttca tgccatggca gcctcagctg catggcctcc cccatcctca
      961 attcgcgtgg cgagacgatc ggcctgctcg atgcgtcctg ctcgaacgag gcgcgccagc
     1021 agcatacgca tgtgctggtg cgaatggcgg cggcgcaaat cgagaatggg ctgatttttc
     1081 aggaacggtc cgagagcttc attttcgcct tccatccgcg cgccgaatat ctggatacac
     1141 tgtcggccgg tttgatcgcg gtgtcgcgcg acggcgaggt cttttctttc aaccggcccg
     1201 gcatcgccct tctcgccggc ctgccgaccg aaccgggcgg ccatttcaac acgctgttcg
     1261 aggccagttt ttccgccgcg atggatgggc tgctccaggg cgggttgatc aagatcaagg
     1321 atcgcgccgg cagcggcctg ttcatggtct gccggcagat cggccagcgc gccgccgtcg
     1381 ccgccaaccg ccgccccaac agggcgcctg cggtcgtggc cgatccgctg gcgccgaatt
     1441 tcgtttgcca ggatgccgcc ctgaagcacg ccatgtcgga catggccgcc gccgtcacgc
     1501 ttaaaatgcc ggtccagatt accggtgaaa ccggaaccgg caaggaactt atggctcgcc
     1561 acgtccatga tttgagccgg cgcaagggcg agttcgtcgc gctcaattgc ggcgcggtgc
     1621 cggaacaatt gttcatctcc gagatattcg gccatgaacg cggcgctttc accaatgccc
     1681 gcaatgaagg cgcgcccggc ctggcgcggc ttgccgataa cggcacgctg tttcttgacg
     1741 aggtggccga cattccgccg gttgcgcaga ccgcgctgct gcgctttctg gattcaatgg
     1801 aagtgcgcac cataggcggg gagaagacgc acaaggtgga tgtgcagatc gtttcggcca
     1861 ccaactgcaa cctccaggag atggtggcgg cgcggacctt tcgtgccgat ctgctctatc
     1921 gcctgaacgc cttcaccgtg cgcctgccgg ccctcgatgc ccggtcggac ttcgcgctga
     1981 tcgtccagca tctcgtccag aagatatcgc cgggcacggc gattaccgat gcggccatcg
     2041 caaagctttc gcagcgcaga tggcccggca acatccgcga gttgcatgcc gtcctgcaac
     2101 gcgcgctggt acgccagcgg atggaatatc tcgacgaggg ctcttttgaa gacatcgccc
     2161 ggaacgatga cgaaagctgc gaggcctgca agccgcatcc gctgaatagg caaagatgcc
     2221 gcgagatcag ggcgacgtac cagctatccg gcgataatgt ctcgaaaacg gcccgcctgc
     2281 tcggtctttc ccgcacgacg atctacaagc acgtacggct ctagccggcg gatcgagtgc
     2341 ttggtctggg tcaaaccgcg agcgttccgg cgcattatcg acgaccggta atgccttccg
     2401 gcactatcga aattaaagtg aacggccttc tcccggattc ggttcatgcc gcctccggga
     2461 tgatcgtgcg cgcaaccttc cgcagcggtc aatcgcgcgg ctttggcggg cgcttcatgg
     2521 tcaaaagccc cgttacatcg tcgaaatcat ccgccgacga gacggcaagc gtcagccatt
     2581 ttccgtctat gtacatcttg gcttcgtcat agagtttgac cagcggcgag cgccaggcat
     2641 agcgtcgctc ctcgaacttc tcccgttccg cgcctcccat gaccaccacc gccgaaaacc
     2701 ggcggtattc cggtatcaag gtggtgaacg ccttggtttt cttgtagcgc aacgaccaac
     2761 cacggttctt gccgccataa agccagtccg gcgcgaaaac gccggggtaa aattcatcga
     2821 tccagttgcg caactcgccc cagcgctcga acgcttccgg tccgatccag ttgcggatgg
     2881 caccatcctc gggcggcgcc gatttatcgg tgatccggtc gccgatttgg ggagctgtat
     2941 caacaggtag aatcatcgaa acaccttgtg tcgtggtgcg gcgcaggccg cgagtttacg
     3001 atccgggcgc cagcggctcg ccgcacgccc tcagggttac cttggcgcga cgcctccaag
     3061 gagcagtcgc ttatgcattc ggaacaaagg acgacggcgg agcgaaactt gaagctgcga
     3121 cggcgcgaag gaaagtcacc tttggccatg ggttcgatag ccgaacgtta tcgtgcgggg
     3181 ccgggccggt cgcaaggtta aagaccgcag gccatgccct cccccgcaac gccgctcctg
     3241 atattcatgg gctggtgggt gtgtggtgga cgcccccaag ggtttcgctt ttgcgttcag
     3301 gttcaggcct gcgcatgcac ccgctcatct actccctgac gatgggctga ttggatttgt
     3361 tggacggttg tctggtcgag ggtgtcgagg gctttttggc gggctttcgg ggcgttgtcg
     3421 tcgtcgatga aggcggtggc ctgtatggac aggaattgca gggatttcag gccgatggag
     3481 ttcagcgcca gcgtcaggta gggggtcacg aaatcgggct ggcttgcgcg ctcgccgcca
     3541 aagacgccgc cggaggaaac ggcgatgaac accggccggt cgcgcagcat cccgaccttg
     3601 ccgttgggcg tcgattgcat cgtgcggccg acacgcagaa cctggtcgat ccaggccttg
     3661 agggcggagg ggatgctgaa gttgtacatc ggcgttccga tgacgatcgc atcggccgcc
     3721 tcgatttccc cgatcagcgc ctcggaaccg tcgagggagc cttcgggcgg attggccagg
     3781 gtggtgggcg aggcgagtgc ggccgcatag agagccggcg tatggggcaa gggttcgctg
     3841 cccaggtcgc gccgggtgat ggtggcgccg ggcgcggcct cgacaagctt tccaacgatc
     3901 gccgccgaaa gtccgcggct atgcgatatt tgtcgcgggc tgacgtcgat gtgaaggata
     3961 ttcatgggat tgatccaatg actttcgggg cggcccggtg gggttcggga cggtgttcgg
     4021 gaagggttcg gattgcgccg caccggaacc gctgtcgcgg tccggcggga gtaaaaaaag
     4081 tcagatcgag ccggccggtg ttacgacggg gaagtccttg tcgtggtcga acacgttgtt
     4141 gaagaagttg gtcagcgaat acatcgccac cagcgccacg atctcgatga tgttggcatc
     4201 ggtgtagccg acg
//
LOCUS       DASYCS010000432         6460 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2882876,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000432 DASYCS010000000
VERSION     DASYCS010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 6460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6460
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2882876"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VGM46_13205"
     CDS             <1..181
                     /locus_tag="VGM46_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013844934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY4193598.1"
                     /translation="AIIATDADFCDYLLTEADVAVVPGSAFGLSPYFRISYATSGDQL
                     KEALQRMKRACERLA"
     gene            complement(523..1269)
                     /locus_tag="VGM46_13210"
     CDS             complement(523..1269)
                     /locus_tag="VGM46_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019996808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY4193599.1"
                     /translation="MNRTAIITGASRGIGRAIALGLAKRGFDLALNDIEHQKQHLLET
                     VEAVKELGRRCVPVFADVSDAAECKRLAQASIDGLGHIDALVNNAGILRMATIEEMTP
                     ELWDQTFAVNTRGVFQMTQSIIGHMKTRQYGRIVNIASLAARTGGPGQAHYAASKSAV
                     VGFTRVSSMEFAGDGITVNAVCPGIIKTEMGMNNLRDPEKIAYFEKVTDVHRLGEPED
                     VVGPVAFFASDDCAFVTGQALNVDGGIFYS"
     gene            complement(1315..2280)
                     /locus_tag="VGM46_13215"
     CDS             complement(1315..2280)
                     /locus_tag="VGM46_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019996807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEY4193600.1"
                     /translation="MAAKFDVSACGSYILDICGFPVTEIPPGGEVALIDEISMTVAGT
                     AGGTVVPCARLGLKAQAFGAIGTDTKGDWIMNTLQKEGIDGSQMMRLDDVQTSATILP
                     IRPDGSRPAIHARGASARWVMTPEAQVEALKAKVLHLGGVNSIVAMQGAPSAALLKDA
                     KASGCITTLDLISARADTLALVEPLLPYVDFFMPSIDEAAVMVGSDDPETCARFFIQK
                     GAGACAISLGADGSYFLNREGRQFSLPAFAVTVRDTSGCGDSYTGGFIAGLVRDWDLE
                     DCAKLATATSAIVATGLGSGANLVSFEKTVEAMNSLPTLQRERKH"
     gene            complement(2420..3115)
                     /locus_tag="VGM46_13220"
     CDS             complement(2420..3115)
                     /locus_tag="VGM46_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019856578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY4193601.1"
                     /translation="MASVIQDEAEARAAIVNSCRDMTALGINQGTSGNISVRAENGIL
                     ITPSGRSYDQMVPEDIISMKWDGTWTGTPGNVPSSEWRFHLDILKSKPEVGAVVHAHP
                     IYCTIIAIMNRPIPAIHYMIAVSGGKDVPCAPYAQYGTQELSERALEALQNRRACLLA
                     HHGLIATGATLKRAMWIAGEVEVLAKQYHGCLQLGEPPLLPDEEIDSILARWGGYGLR
                     EKAKQPEAISASN"
     gene            complement(3318..5633)
                     /locus_tag="VGM46_13225"
     CDS             complement(3318..5633)
                     /locus_tag="VGM46_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY4193602.1"
                     /translation="MGEIKRVPHCSHWGAYTLLVEDDTIVDVEPFEFDPAPSPIINSV
                     KEWASDSRRVPGPLVRSGWLEKRENSDRRGRGNEKFVPVSWDEATTLIADEIKRVSRD
                     FGNASIFAGSYGWTSCGRFHHAPSLLKRMMNLAGGYTGHVDTYSTAAGPVILRHILGD
                     DSACNGQSNTLDTIAEHTETLVVFGAMSPRTAQNEAGGIASHSLETYLAKIAKRGVKV
                     ILVSPFRDDMPDWVNAEWWPIRPNTDTALMIGLAGEILRAGRHDASFLERCTSGSDRF
                     LAYLNGESDGVAKTAEWCAEITGLDRDQIASLARQLVDTRSMITVSWSLQRAHHGEQP
                     FWAALALASMIGQVGLPGGGVGYGYASLGGVGAPINIAKSPAMSQLSKRIDSFIPVAR
                     ISDMLLNPGQTFTYEGAVRSYPDTRLVYWAGGNPYHHHQDLNRLSEAWTRPETIIVQD
                     PMFTATAQRADIVLPANTSIERNDIAGNKRSDFVIAMHQAVASVGDSKSDFDIFNAIA
                     IKLGVGDGFNEGRDEMGWLRHVYDISRADAQTRLSFEMPDFDTFWKQGYARCPTVDQN
                     VYLADFREDPKGHILNTESGKIVIGSDTLAALNYSDCREHPAWIEPAEWLGSAQARDQ
                     LHLISHQPAGRLHSQLETGPASMAEKRNGREQARINPQDARDLGITDGQSMRLWNDRG
                     ECLATAHVTDTVRQGVVILPTGAWFTPSGNNGLEIAGNPNVLTLDVGTSQFGQGCSAH
                     TCLVHIEPYFGDVADAAQTYLDKLNSLAVGT"
     gene            complement(5650..>6460)
                     /locus_tag="VGM46_13230"
     CDS             complement(5650..>6460)
                     /locus_tag="VGM46_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019996804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEY4193603.1"
                     /translation="LDRVPYVIDKALEVFADTEQVLLVGAKSPVGFFAYPGKPSSMLP
                     ETCTVMELALEGEDLVAAIEMLADEIGVKGITPRLAGRETPPLPDGRLTPAAIAASLT
                     AHMPENAIICDESVSSGRDFFAPTYGANPHDFLQLTGGSIGIGLPLATGAAIACPDRK
                     VVVLQADGSGMYTVQALWTQAREELDVLTIVFANRRYQILLGELKNVGAGVPGENASR
                     MLNLDSPALNWVQIANGMGVEAARADTVERFDDLLKSACARKGPFLIEAMI"
BASE COUNT         1157 a         1977 c         1997 g         1329 t
ORIGIN      
        1 cgcgatcatc gcgaccgacg ccgatttttg cgattacctt ttgaccgagg cggacgtggc
       61 ggtggtcccc ggttcggcat ttggcttgtc gccgtatttt cggatttcgt acgccacctc
      121 cggcgatcag ctgaaggaag cgctgcaaag gatgaagcgg gcatgtgaac gcctcgcata
      181 gcggtttcgt cggaaattcg gccaatcggt tttgacgttc aaactgtgat cgctaccgcc
      241 ttgagcggcg aaatcgaact gcaatcgccc gcccatcgta tccatcagac gaagggcggg
      301 gcgattgcgt ttgcgaacgc tagaaggcgc atggttggga ggcttgtgtc atgctctttg
      361 gaaaccgctt ggcaagcgtg ccgtgcagaa ccgcgcgcgc ggtagttgcc ggcctttaac
      421 ctcttggcca gcggcgaaga ggcgatagcc gaccggcttg ccgctgacaa aggcggcgcg
      481 attatcgcgc gccgccccgg cacgggatgg cgaaagaaag cctcagctgt agaaaatgcc
      541 gccgtcgacg ttgagcgcct gtccggtgac aaaagcgcaa tcgtcggagg cgaagaacgc
      601 caccggcccg accacgtctt ccggttcgcc cagccgatgc acgtcggtga ccttctcgaa
      661 ataggcgatc ttttcggggt cgcgtagatt gttcatgccc atctcggtct tgatgatgcc
      721 ggggcaaacc gcgttgacgg tgatgccatc gccggcgaat tccatggaag acacgcgcgt
      781 gaagccaacc accgccgact tcgatgcggc gtagtgggcc tggccgggtc caccggttct
      841 ggcggccagc gaggcgatgt tgacgatacg gccgtattgc cgcgtcttca tgtggccgat
      901 aatggattgg gtcatctgga acacgccgcg ggtgttcacg gcaaaggtct ggtcccaaag
      961 ctcgggcgtc atttcctcga tggtcgccat tctcaaaata ccggcattgt tgaccagagc
     1021 gtcgatgtgg ccaagcccgt cgatggatgc ctgcgcgagc cttttgcatt cggcggcatc
     1081 gcttacgtcg gcaaacaccg gcacgcagcg ccggcccagt tccttgaccg cttcgaccgt
     1141 ttcaagcagg tgctgctttt gatgttcgat atcgttgagg gcgaggtcga agccgcgctt
     1201 tgccagaccg agcgcaatcg cgcgcccaat gccacggctt gcacccgtaa tgattgcagt
     1261 tctgttcatt tctttcctat tcccgattgt ctgacggttt ttttggggca agctttagtg
     1321 cttgcgctcg cgctggaggg tcggcagcga gttcatcgcc tcgacggttt tttcgaagga
     1381 aaccagattt gcgcccgagc ctagcccggt cgcgacgatg gctgaagtcg ccgtggcgag
     1441 cttcgcgcag tcttccaggt cccaatcccg gaccaggccg gcgatgaagc cgccggtata
     1501 ggaatcgccg cagccagacg tatcccgcac cgttaccgca aaagcgggaa ggctgaactg
     1561 ccgtccctcc ctgttcagga agtaggagcc atcggcgccg agagagatgg cgcacgcgcc
     1621 ggcgcccttt tgaatgaaga agcgggcgca tgtttccgga tcgtcgctgc ccaccatcac
     1681 ggcggcctcg tcaatgctgg gcatgaagaa gtcgacatag ggaagcagcg gctccaccag
     1741 cgccagcgta tcggcgcgcg ccgaaataag gtccagagtg gttatgcagc cggacgcctt
     1801 ggcgtctttc agaagggcag ccgaaggcgc gccttgcatg gcgacgatgg aattgacgcc
     1861 gccaaggtgc agaaccttgg ctttgagcgc ctcgacctgt gcttcgggcg tcatcaccca
     1921 gcgggcggag gctccgcgcg catgaattgc cggccttgag ccatcgggcc ggatcggcag
     1981 aatcgtggcc gaagtctgca catcgtcgag gcgcatcatt tgcgagccgt cgatcccttc
     2041 cttttgaagg gtgttcatta tccagtcgcc cttcgtgtcg gtgccgatcg caccgaaggc
     2101 ctgggccttg aggcccagtc gcgcgcacgg aaccacggtg ccgccggcgg tgccggcaac
     2161 cgtcatgctg atctcgtcaa tcagcgcaac ttcgccgccc ggcggaatct ccgtcaccgg
     2221 gaaaccgcaa atatccagaa tgtaacttcc acacgcgctt acgtcgaact tggcagccat
     2281 gacggcttcc ctcacttcat ggtttggttg aaatcttata gcggttccga tttttactta
     2341 atcgacaaaa ccgctctatc tctttgtttt ggccgcattt ccggacgcaa aaccgtttca
     2401 cacttttgct ggaaatgctc tagttggatg cggagatcgc ctcgggctgc ttggcttttt
     2461 cgcgcaagcc atatccgccc caccgcgcaa ggatgctgtc gatctcttca tcgggcaaaa
     2521 gcggcggctc gccgagttgc aggcagccat gatactgctt ggcaagcacc tccacctcac
     2581 ccgcgatcca catcgccctt ttcaacgtgg ctccggttgc gatgaggcca tggtgggcca
     2641 gaaggcaggc gcggcggttc tgcaacgcct ccagcgcgcg ctcggacagt tcctgtgtgc
     2701 cgtactgggc gtaaggggcg cagggcacgt cctttccgcc tgacacggca atcatgtagt
     2761 ggattgccgg gatcggacgg ttcatgatcg cgatgatcgt gcaatagatc ggatgcgcgt
     2821 gaacgacggc cccgacctcg ggtttggact tgaggatgtc gagatggaaa cgccactcgg
     2881 acgaggggac atttcccggc gtgcccgtcc aggtgccatc ccatttcatc gagatgatgt
     2941 cctcgggcac catctggtcg tacgacctgc ccgatggcgt gatcaggatg ccgttttcag
     3001 cgcgcacgct gatgttgccg gacgttccct ggttgatgcc aagcgccgtc atgtcccggc
     3061 acgaattgac gatcgcggcg cgcgcttcgg cctcgtcctg gattacgctt gccatgccgg
     3121 tcgcccctct gtggcggccg cggcttcgat ccggctcgcg ccgcgcgcgg ccatcgccgc
     3181 ctggcccggt aaatcctcat gttccatttt catcgttcct tcacccgcca tgcaggtctg
     3241 tcaaaatccc tgggcatgca aaatcgaaag ccctcgcggc cgcctcggcg cttgcgcctc
     3301 aacgtcgctt caggcggtca tgtgccaacc gccaggctgt tcagcttgtc cagatatgtc
     3361 tgggccgcat ccgctacatc gccgaaatat ggttcgatat ggaccaggca tgtatgggcg
     3421 gagcaccctt gcccgaactg cgatgtgccg acatccaatg tcagcacatt ggggtttccg
     3481 gcaatctcaa ggccgttgtt tccggacggc gtgaaccacg cgccggtggg aaggatgacc
     3541 acgccctgcc tgaccgtatc ggtcacatgg gcggttgcca ggcactctcc ccgatcgttc
     3601 cacaagcgca tggactgacc gtcggttatc cccaggtcac gggcgtcctg tgggttgatg
     3661 cgcgcctgtt cccggccgtt ccgtttctcg gccatgcttg ccggaccggt ttccaattgg
     3721 ctgtggagcc gcccggctgg ctggtgcgag ataagatgga gctggtcgcg ggcctgcgcg
     3781 gagccaagcc attccgccgg ttcgatccag gcgggatgct cgcggcagtc ggaatagttc
     3841 aaggcggcaa gcgtatcgct gccgatcacg atctttccgc tttcggtatt gaggatgtgg
     3901 cccttggggt cttcgcgaaa atcggccagg tacacattct ggtccaccgt tggacacctg
     3961 gcatagccct gtttccagaa agtgtcgaaa tcgggcattt caaacgacag ccgcgtctgc
     4021 gcatcggcgc ggcttatgtc gtagacatgc ctcagccatc ccatctcgtc gcggccttcg
     4081 ttgaagccgt cgcccacgcc gagcttgatc gcaatggcat tgaaaatatc gaaatcggac
     4141 ttggaatcgc cgacgctggc aaccgcctga tgcatcgcaa tgacaaaatc ggaacgcttg
     4201 tttccggcga tgtcgttgcg ctcgatcgat gtgtttgcgg gaaggacgat gtcggccctc
     4261 tgcgccgtgg ccgtgaacat cggatcctgg acgatgatcg tctcgggccg cgtccaggct
     4321 tccgaaagcc ggttgaggtc ctgatggtgg tggtagggat tgccgcccgc ccagtagacg
     4381 agccgggtat cgggataaga ccggaccgcg ccttcgtaag tgaaggtttg tccggggttc
     4441 aacagcatgt cgctgatacg cgccaccggt atgaaactgt cgatgcgctt gctcagctgg
     4501 gacatcgccg gcgacttggc gatgttgatg ggcgcgccga cgccacccag cgaggcatag
     4561 ccgtatccga cgccgccgcc cggcaggccg acctggccga tcatggatgc aagcgccaac
     4621 gccgcccaga acggctgttc gccatgatgg gcgcgttgca ggctccagct gaccgtgatc
     4681 atcgagcgcg tatcgaccaa ttgccgcgcc agcgaggcga tctggtcgcg gtcgaggcct
     4741 gtaatttcgg cgcaccattc ggctgtcttc gccacgccgt cgctttcgcc gttcagatag
     4801 gccagaaagc ggtccgaccc gctggtgcag cgctctagga acgacgcatc gtgccggccg
     4861 gcgcgcaata tctcgccagc caggccgatc atcagggcgg tgtcggtgtt ggggcgtatc
     4921 ggccaccact cggcattgac ccagtccggc atatcgtcgc ggaacgggga aacgaggatg
     4981 accttcacgc cacgcttggc aatcttggcc agataggtct caagcgaatg gctggctatt
     5041 cctccggctt cgttttgcgc cgtcctgggc gacatggcgc cgaaaacgac gagggtttcg
     5101 gtgtgttcgg cgatggtatc gagcgtgttc gactgtccgt tgcatgcgga atcgtcgccc
     5161 aatatgtggc gcagaatgac cgggccggca gcggtggaat aggtgtcgac gtggccggta
     5221 tacccgccgg cgaggttcat catccgcttg agcaatgagg gcgcgtggtg aaaccggccg
     5281 cagctcgtcc agccatacga tccggcaaag atcgaggcat tgccgaagtc gcgcgacacc
     5341 cgctttatct catcggcaat cagggtggtt gcctcgtccc agcttacggg aacgaacttc
     5401 tcattgccgc gcccccggcg gtcgctgttc tcgcgctttt ccagccagcc ggagcgcacc
     5461 agggggccgg gtacgcggcg gctgtcgctc gcccactcct tcaccgaatt gatgatgggc
     5521 gacggcgccg gatcgaactc gaagggttcg acgtcgacga tcgtgtcgtc ttccaccaga
     5581 agcgtatagg cgccccagtg gctgcaatgc ggtactcgct tgatctcacc cataacaggc
     5641 accttctcgt tagatcatag cctcgataag gaacggccct tttcgggcgc aggcggactt
     5701 cagcaggtcg tcgaaacgct cgaccgtatc ggcccttgcc gcttcgacgc ccatgccgtt
     5761 ggcgatctgg acccagttca gcgcggggct gtcgaggttg agcatgcggc ttgcgttctc
     5821 gcccggcacg ccggcgccga cattcttcag ttcgcccaga agtatctggt aacggcggtt
     5881 ggcaaacacg atggtaagca cgtccagctc ctcgcgggcc tgggtccaaa gagcctgcac
     5941 cgtatacatc ccgctgccgt cggcttgaag cacgacgact tttctatcgg gacaggcaat
     6001 cgccgccccg gtcgccagcg gaagcccgat gccgatcgat ccaccggtga gttgcaggaa
     6061 gtcatgcggg ttggccccat atgtcggggc aaagaagtcc cggcccgaac tgaccgactc
     6121 gtcgcaaatt atcgcgttct caggcatgtg ggcggtaagg gaagcggcga tggcggcggg
     6181 tgtcagccga ccgtccggca agggcggggt ttcgcgcccg gccagtcgcg gggtgatgcc
     6241 cttgaccccg atttcgtcgg ctagcatctc gatggccgcg acaaggtctt ccccctccag
     6301 cgccagttcc atgacggtac atgtttcggg aagcatgctg cttggtttgc ccggatacgc
     6361 aaaaaagccg accggtgatt tcgcaccgac cagcaacacc tgctcggtgt cggcaaacac
     6421 ctcaagcgcc ttgtcgatca cgtaagggac gcggtcgagc
//
LOCUS       DASYCS010000433         4049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_417423,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000433 DASYCS010000000
VERSION     DASYCS010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4049
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417423"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="VGM46_13235"
     CDS             <1..795
                     /locus_tag="VGM46_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003610550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY4193604.1"
                     /translation="ATHKPQRVVNLAAQAGVRYGFQNPHAYIDSNIVGFLHILEGCRN
                     HGVEHLVYASSSSVYGANTKMPFSVHDNVDHPVSLYAASKKANELMAHSYSHLYGIPV
                     TGLRFFTVYGPWGRPDMSPFIFTRKILAGEPIDVFNHGRHSRDFTFIDDIVEGVVRTL
                     DAVARPDAGWDSDNPEPSTSSAPYRIYNIGNNQPVELLYFIECIENAVGKRAQKNMLP
                     AQQGDVLRTFADIDGLVADIGFQPRTSIEDGTRRFVEWYKGFYGIV"
     gene            1033..1938
                     /gene="cysD"
                     /locus_tag="VGM46_13240"
     CDS             1033..1938
                     /gene="cysD"
                     /locus_tag="VGM46_13240"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010914955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEY4193605.1"
                     /translation="MHAPLTHLQRLEAESIHIFREVAANFSKPVMLYSVGKDSSVLVH
                     LAMKAFYPAKPPFPFLHVDTTWKFREMIAFRDVMAEKLGFELIVHINEDGVRDNINPF
                     DHGSNVHTHVMKTVGLRQALDKYGFDAAFGGARRDEEKSRAKERIFSFRNASHAWDPK
                     NQRPEMWNIYNTRVAAGESIRVFPLSNWTELDIWQYILQEQIPIVPLYFARPRPVVER
                     DGMLIMRDDERMNLLPGETIENRTVRFRTLGCYPLTGAIDSEADTLEAIVSEMLTART
                     SERQGRLIDRDEAGSMEKKKREGYF"
     gene            1941..3839
                     /gene="cysN"
                     /locus_tag="VGM46_13245"
     CDS             1941..3839
                     /gene="cysN"
                     /locus_tag="VGM46_13245"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HEY4193606.1"
                     /translation="MASLAEKDSAAIGDVRDYLAAQEKKTLLRFLTCGSVDDGKSTLI
                     GRLLSDTRQIFEDQLAALERDSRKHGTTGEDVDFALLVDGLEAEREQGITIDVAYRFF
                     ATPKRKFIVADTPGHEQYTRNMATGASTADLAIVLIDARQGVLRQTRRHSIIASLLGI
                     RHIVLAVNKIDLMDHSQAVFDQIVTDYMEFARDLGFKSIQPIPMSARFGDNVTMASPK
                     MPWYSGPTLLGHLETVPVENDYLDKPFRFPVQHVVRPNLDFRGFAGQVASGKVAIGDK
                     VTVAKSGQQSRIKEIVTHGGNLESAVEGQAVTIVLADQIDISRGDMLVDPTDRPHVAD
                     QFQAHVIWFDAQPMIPGRSYILRTEVDSVSATVTALRHQVNINTFAHEAAKHLNMNEV
                     GECNISTQAPIAFDAYKDNRVSGNFVLIDRFTNATVGAGMIDFPLRRASNVHWQAMEV
                     NKDARAALKSQRPTVLWFTGYSGSGKSTIANNLEKLLAAQGRHTFMLDGDNVRHGLNR
                     DLGFTDDDRVENIRRVSEVAKLMTDAGLIVIVSFISPFRSERQMARDLFPQGEFIEVF
                     IDTPLEECIKRDPKGLYRKAQAGEIENFTGISSPYEAPETPEVHIHTLGHEPAELAML
                     IETYLNKR"
     gene            3845..>4049
                     /locus_tag="VGM46_13250"
     CDS             3845..>4049
                     /locus_tag="VGM46_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015916941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEY4193607.1"
                     /translation="MIETFETLALAAGKAILDVYHAGAHVSYKADSSPVTEADERAEA
                     IILAGLKQAFPDIPVVAEEAAATG"
BASE COUNT          839 a         1337 c         1156 g          717 t
ORIGIN      
        1 gccacgcaca agccgcagcg tgtcgtcaac ctcgccgcgc aagctggtgt ccgctacggc
       61 ttccagaacc cgcacgccta tatcgacagc aacatcgtcg gcttcctcca tattctggag
      121 gggtgccgga accacggcgt cgagcatctc gtctatgcgt cttcaagttc ggtctatggc
      181 gcgaatacca aaatgccgtt cagcgtccac gacaatgtcg accatcccgt cagcctttac
      241 gccgccagca agaaggcgaa cgaactgatg gcgcattcct attcgcatct gtacggcata
      301 ccggtcaccg gcctgcgctt tttcacggtc tacggtccgt ggggcaggcc cgacatgtcg
      361 cccttcatct tcacccgcaa gatattggcg ggcgagccga tcgacgtgtt caaccatggc
      421 cggcattcgc gcgacttcac cttcatcgac gacatcgtcg aaggcgtggt gcgcacgctg
      481 gacgccgtgg cgcggccgga tgccggctgg gacagcgaca atcccgagcc atccacctcg
      541 tcggcgccct accgcatcta caatatcggc aacaaccagc cggttgaact gctctatttc
      601 atcgaatgca tcgaaaacgc cgtcggcaag cgcgctcaaa aaaacatgct gccggcccag
      661 caaggcgacg tgctgaggac ctttgccgat attgacggac tggtggcgga tatcggcttt
      721 cagcctcgaa cctcgatcga agacggcacc cggcgctttg tcgaatggta caagggtttc
      781 tacggcatcg tctgaatcgc cggagatggc gcgggcggtg gccgcattcg ggccaaaccg
      841 gcaaggctgg cgatggagcg ggtaaaaatt gccgcttgcg aaccaatcgc agaaaattcg
      901 tttagccagc ctgctgccgc gagtgtgtag cgcattggcc tcgctggccg ccaactgcta
      961 aaagcggtca aactgaatga ccgggcgtca aggaccatca gcggtccggc gcgatgaaag
     1021 aatacgccgt tcatgcacgc tccacttacc caccttcagc ggctcgaagc tgaatcgatc
     1081 catatattcc gcgaggtcgc ggcgaacttt tccaagccgg tgatgcttta ttccgtcggc
     1141 aaggattcct ccgttctggt gcatctggcg atgaaggcat tctatccggc caagccgcct
     1201 tttccgttcc tgcatgtgga tacgacctgg aaattccgcg aaatgatcgc gtttcgcgac
     1261 gtcatggccg aaaagctcgg cttcgagctc atcgttcaca tcaacgagga tggcgtgcgc
     1321 gacaacatca acccgttcga ccacggttcc aacgtccata cccatgtgat gaaaacggtg
     1381 ggcctcaggc aggcgctcga caaatacggc tttgacgcgg ccttcggcgg ggcgcgccgc
     1441 gacgaggaga agtcgcgcgc caaggagcgc atcttttcct tccgcaatgc cagccatgcc
     1501 tgggacccga aaaaccagcg gccggaaatg tggaatatct acaatacgcg cgtggcggcg
     1561 ggcgaatcga tccgggtttt tccactgtcg aactggaccg aactcgatat ctggcaatat
     1621 atcctgcaag aacaaatccc catcgtgccg ctttatttcg ccaggccgcg cccggttgtc
     1681 gaacgcgacg gcatgctcat catgcgcgac gacgagcgca tgaacctgct gcccggcgag
     1741 acgatcgaga accggaccgt gcggttccgc acgctgggct gctacccgct gaccggcgcc
     1801 atcgattccg aagcggatac gctggaagcc atcgtcagcg aaatgctgac cgcccgcacc
     1861 tccgaacggc agggccgcct catcgaccgc gacgaggcgg gttcgatgga gaagaagaag
     1921 cgcgaggggt atttctgagc atggccagcc tcgccgaaaa agatagcgcc gccataggcg
     1981 acgtgcgtga ctatctggcc gcgcaggaaa agaagacgct gctgcgcttc ctcacctgcg
     2041 gctcggtgga cgatggcaag tcgacgctga tcgggcggct tctttccgat accaggcaga
     2101 tcttcgagga ccagcttgcc gcgcttgaac gcgattcgcg caagcacggc accaccggcg
     2161 aggatgtcga tttcgcgcta ttggtcgatg ggctggaggc ggagcgcgag cagggcatca
     2221 ccatcgatgt cgcctaccgc ttcttcgcta cgccaaagcg caagttcatc gtcgccgaca
     2281 cgcccggcca cgagcaatat acgcgcaaca tggcgaccgg cgcctcgacc gccgacctcg
     2341 ccatcgtgct gatcgacgcg cgccagggcg tgctgcgcca gacccggcgc cattcgatca
     2401 tcgcctcgct gctcggcatc cgccacatcg tgcttgcggt caacaagatc gacctgatgg
     2461 atcattccca ggcggtgttc gaccagatcg tcaccgacta catggaattc gccagggatc
     2521 tcggtttcaa gagcatccag ccgatcccga tgtcggcgcg cttcggcgac aatgtcacca
     2581 tggcttcgcc gaaaatgccc tggtacagcg gcccgaccct gctcggccat ctcgaaaccg
     2641 tgccggtcga aaacgactat ctcgacaaac ccttccgttt cccggtgcag cacgtcgtcc
     2701 gcccgaacct cgatttccgc ggtttcgccg gccaggtcgc ctcgggcaag gtcgccatcg
     2761 gtgacaaggt caccgtcgcc aagtccggcc agcaatcgcg catcaaggag atcgtcaccc
     2821 atggcggcaa tctcgaaagc gccgtcgaag gccaggccgt caccatcgtg ctcgccgacc
     2881 agatcgacat ttcacgcggc gacatgctgg tcgatccgac cgaccgaccg catgtggccg
     2941 accagttcca ggcgcatgtc atctggttcg acgcccagcc gatgatcccg ggccgcagct
     3001 atatcctgcg caccgaagtc gacagcgtca gcgccacggt gaccgcgctc aggcaccagg
     3061 tcaacatcaa caccttcgcc cacgaggccg ccaagcacct caacatgaac gaggtcggcg
     3121 agtgcaatat ctcgacccag gcgccgattg ccttcgacgc ctacaaggac aaccgcgtca
     3181 gcggcaattt cgtgctgatc gaccgcttca ccaacgccac cgtcggcgcc ggcatgatcg
     3241 actttccgct gcgccgcgcc tccaacgtcc actggcaggc gatggaggtc aacaaggacg
     3301 cgcgtgccgc gctcaagagc cagcggccga ccgtgctctg gttcacaggc tattccggct
     3361 cgggcaagtc gaccatcgcc aacaacctcg aaaagctgct ggccgcccag ggcaggcaca
     3421 cgttcatgct cgacggcgac aatgtccgcc acggcctgaa ccgcgatctc ggtttcaccg
     3481 atgacgaccg cgtcgaaaac atccgtcgcg tctcggaagt cgccaagctg atgaccgatg
     3541 ccggcctgat cgtcatcgtg tccttcatat cgcccttccg atccgaacgg caaatggcgc
     3601 gcgatctgtt cccgcaaggc gagttcatcg aggtgttcat cgatacgccg ctggaagaat
     3661 gcatcaagcg cgatccgaag ggtctctaca ggaaggcgca ggccggcgag atcgagaatt
     3721 tcaccggcat ctcctcgccc tacgaggcgc cggaaacccc ggaagtgcac atccataccc
     3781 tcggccacga gccggcggaa ctggccatgc tgatcgaaac ctatctcaac aagcggtgag
     3841 cgccatgatc gaaacattcg aaacgctcgc ccttgccgcc ggcaaggcaa tcctggatgt
     3901 ctaccacgcc ggcgcccatg tcagctacaa ggccgacagc tcgccggtga cggaagccga
     3961 cgagcgcgcc gaggcgatca ttctcgccgg cctcaagcag gcctttccgg acatccctgt
     4021 cgtcgccgaa gaagcggctg cgaccggca
//
LOCUS       DASYCS010000434         3974 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2738430,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000434 DASYCS010000000
VERSION     DASYCS010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3974
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2738430"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..1207
                     /locus_tag="VGM46_13255"
     CDS             83..1207
                     /locus_tag="VGM46_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193608.1"
                     /translation="MGPSSSNVSAWTDQYFLKTKATVQRFGDMCVTYAVFMRRPVVSA
                     PRIALNWLHEMARERGTEFRVDLIYPEGKWVGAGEPVMYITGSLVHLSDLETILLMKL
                     GPACVAAFNASTMCSDLPSAAFLAMDARHCAGLEMAELMAYAASVGSARARRKVGAKG
                     FIGNATEATAHYFGQEHAMGTMPHALIGYAGSTLRAAEMFHETFPEQPMTVLVDYFGR
                     EVTDSLTVARRFPNLAAEGRLSVRLDTPGSRFIEGLDPSVSYAIMERHVPRVIRGYRS
                     EQELRHLVGAGVSASAIWHLRDALNREGFQRVKIVASSGFDPAKCKVMAEALAPIDVI
                     GTGSFLPQRWTETYATADIIEYDGVKRVKIGREFLFRKQD"
     gene            1432..1761
                     /locus_tag="VGM46_13260"
     CDS             1432..1761
                     /locus_tag="VGM46_13260"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HEY4193609.1"
                     /translation="MPHYMLQGSYTKEAIKAMAAKPEDRSVPAGKLAKSLGGKLHSLF
                     MSFGKYDFVAIMELPDDEAAAACSMTVASAGHVAHMSTTKLFTVAEATKAMAKAGASK
                     IDAPKGK"
     gene            complement(1811..2098)
                     /locus_tag="VGM46_13265"
     CDS             complement(1811..2098)
                     /locus_tag="VGM46_13265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193610.1"
                     /translation="MALSAAIGVTHHAIAQQNYPDQLTPTPDIEKVGQRYILMVLDQP
                     DRDLAQGRTKVNVMVDTRTGKTWILQYARKPSSNELGYVWSEIPFAKAPGE"
     gene            2328..>3974
                     /locus_tag="VGM46_13270"
     CDS             2328..>3974
                     /locus_tag="VGM46_13270"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012109980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEY4193611.1"
                     /translation="MTDTLRISFAQFNPTVGDIAGNADLVRKARRHAAEDSADLVMLS
                     ELFLSGYPPEDLVLKPAFQEACRAATEALAAETADGGPAVLLGTPWVEEGKLYNAICL
                     LDGGKVAATRYKVDLPNYSVFDEKRVFAAAGLQGPVSFRDVRLGLPICEDIWKPEVVE
                     CLVESGADILLVPNGSPYEYDKQDVRYTLVRQRIEESGRPAVYLNQVGGQDELVFDGA
                     SFVVNGDGQIAVQMPAFAEAQTTTTWRRVGNNGWKCDAGLLAPQPDQLEGIYQAMMLG
                     LRDYVNKNRFPGILLGLSGGIDSAISAAVAVDALGADKVRAFMLPSPYTSDDSIDDAA
                     QCAELLGITHDTVSIQPAMHAFEEMLRDVFAGRNQDVTEENVQSRIRGVSLMAISNKL
                     GHMLLTTGNKSEMSVGYATLYGDMCGGFSVLKDIYKIMVYRLCKWRNAHKPANALGPS
                     GPVMPERVLTKAPTAELRPNQTDQDSLPPYEILDGILECLVEKELTVEAIVAKGFDRA
                     TVLRVWRMLDLAEYKRRQAPPGVKITHRAFGKDRRYPITNG"
BASE COUNT          716 a         1254 c         1260 g          744 t
ORIGIN      
        1 gcattcctgg ttgccgagca attgaaagcg gaacgggtcg gtcatggaca gcggatcgcc
       61 gaagcctcct gaagggccgg ccatggggcc gtccagttcg aacgtttcgg cctggacgga
      121 ccagtacttc ctgaagacca aggcgacggt ccagcgcttc ggcgatatgt gcgtcaccta
      181 tgccgtcttc atgcggcgcc cggtggtctc cgcgccgcgc atcgcgctga actggctgca
      241 tgaaatggcc agggagcgcg gcacggaatt cagggtcgat ctcatttacc cggaagggaa
      301 gtgggtcggc gccggtgaac cggtcatgta tatcaccggc tccctcgttc atctgtccga
      361 tctcgagacc atcctgctga tgaagctggg gccggcttgc gtcgcggctt tcaacgcctc
      421 caccatgtgc tccgacctcc ccagcgccgc cttcctcgcc atggatgcgc ggcactgcgc
      481 cggcttggag atggccgaac tgatggccta tgcggcttcg gtcggttccg cccgcgccag
      541 gcgcaaggtt ggcgccaagg gcttcatcgg caacgccacc gaggcgacgg cgcattattt
      601 cggccaggaa catgccatgg gcacgatgcc ccatgcgctg atcggctatg ccggttcgac
      661 cttgcgggcg gccgagatgt tccatgagac tttcccggaa cagccgatga ccgtgctggt
      721 cgattatttc ggccgcgagg tcacggattc cctcaccgtg gcgcggcgct ttcccaatct
      781 cgcagccgag ggtcggctct ctgtccgtct cgatacgccg ggcagccgct tcatcgaagg
      841 gctcgacccc agcgtttcct acgcgatcat ggagcggcat gtgccgcgtg tgatccgtgg
      901 ctatcgcagt gagcaggagt tgcgccatct ggtcggcgcc ggcgtatcgg cttccgcgat
      961 ctggcatctg cgcgacgcgc tcaaccgcga gggcttccag cgggtgaaga tcgtggcctc
     1021 ttccggcttc gatccggcga aatgcaaggt gatggcggag gccttggcgc cgatcgacgt
     1081 catcggtacc ggttccttcc tgccgcagcg ctggacggag acctatgcca cggcggatat
     1141 catcgaatat gacggcgtga agcgcgtcaa aatcggccgc gagttcctgt ttcgtaagca
     1201 ggactgaagc cgcttacgcc attcggagca gttcccggcg actctacgcc gaacgaaggt
     1261 gcttaacgcc tgcgttcggc ctttctatga tcttgcggtc gagaccctgg atttgcgttt
     1321 cctgcgtccg acccgccacc atcaatcgca accggacccg gccaaccacg gtgcctcgac
     1381 cgacagaccc gttgtcaacc gaccgcgggc gcaaccccat gggagggcac catgccgcat
     1441 tacatgttgc aaggcagcta tacgaaggaa gcgatcaagg ccatggcggc gaagccggaa
     1501 gaccggtccg tgccggccgg caagctcgcg aagagcctgg gcggcaagct gcacagcctg
     1561 ttcatgagct tcgggaagta tgatttcgtc gccatcatgg aattgccgga cgatgaagcg
     1621 gcggcggctt gcagcatgac cgtcgcttcg gccggccatg tcgcgcatat gtccaccacg
     1681 aaactgttca ccgtggccga agcgaccaag gcgatggcca aggccggtgc ttccaagatc
     1741 gacgcgccga aagggaaata gcggcgaccg gatgccggaa tggcgcgtca cgtccgcgaa
     1801 cgtggcgcgc ttattccccg ggcgccttgg cgaaggggat ttccgaccag acatagccca
     1861 gctcgttgct gctgggcttg cgggcatatt gcaggatcca ggtcttgccc gtccgggtat
     1921 ccaccatgac attgaccttg gtgcggccct gggcgagatc ccggtccggc tggtcgagca
     1981 ccatcaagat ataacgctgg ccgacctttt cgatatcggg agtcggcgtc aactggtccg
     2041 gatagttctg ttgggcaatg gcgtgatggg taacgccgat ggctgcgctt aaagccacga
     2101 cagcaaacag cgctttccgc tttggtaaat ggcgcaagat ggtctccccg caaaaatcgt
     2161 ccctggatag gattttcccc cggcctttcg cgggtgccaa gctgcaattc tcaggcggca
     2221 gggcgggaat gccgcaaaat catggcgttt caacaccttt gctgcgctgc cgcattgaaa
     2281 gtcccggagc cgcccggtaa ggttttccag gacaaggcag gattcccgtg accgatacgc
     2341 ttcgcatctc tttcgcccag ttcaatccca ccgtcgggga tatcgccggc aatgcggacc
     2401 tcgttcgcaa ggcgcgccgc catgccgccg aggattccgc cgatctcgtc atgttgagcg
     2461 agcttttcct ctccggctat cccccggagg acctggtcct gaagccggct ttccaagagg
     2521 cctgccgcgc cgcgaccgag gcccttgccg ccgaaaccgc cgatggcggg ccggccgtcc
     2581 tgctgggcac gccctgggtg gaagagggca agctctacaa cgcgatttgc ctgctggacg
     2641 gcggcaaggt cgcggccacc cgctacaagg tcgatctgcc gaattacagc gtgttcgatg
     2701 aaaagcgggt cttcgccgca gccggcctgc agggtccggt cagtttccgc gacgtccgcc
     2761 tcggtctgcc gatctgcgag gatatctgga agccggaagt cgtcgaatgc ctggtggaat
     2821 ccggcgccga catcctgctg gtgccgaatg gcagccccta tgaatacgac aagcaggatg
     2881 tgcgctacac cctggtgcgt cagcgcatcg aggaatccgg gcggccggcg gtctatctga
     2941 accaggtggg cggccaggat gagttggtct tcgacggcgc cagcttcgtc gtcaacggcg
     3001 acggccagat cgcggtgcag atgccggctt tcgccgaggc gcaaaccacc acgacctggc
     3061 gccgtgtcgg caataacggc tggaaatgcg atgccggcct gcttgcgcca cagccggacc
     3121 agctcgaggg tatctatcag gcgatgatgc tgggcctgcg cgactatgtg aacaagaacc
     3181 gcttcccggg catcctgttg ggcctttccg gcggcatcga ttccgcgatc tcggcggccg
     3241 tcgctgtaga cgcactcggc gccgacaagg tgcgggcctt catgctgcct tcgccttata
     3301 ccagcgacga cagcatcgac gatgccgcgc aatgcgccga gctgctcggc atcacgcatg
     3361 acacggtttc gatccagccg gcgatgcacg ctttcgagga aatgctgcgc gacgtcttcg
     3421 ccggccgcaa ccaggatgtg accgaggaaa atgtgcagag ccgcatccgc ggcgtctcgc
     3481 tgatggcgat ctccaataag ctcggccaca tgctgctgac cacaggcaac aaatccgaga
     3541 tgtcggtggg ctatgccacg ctctacggcg atatgtgcgg cggcttctcc gtcttgaagg
     3601 atatctacaa gatcatggtc taccggctct gcaagtggcg gaacgcgcat aagccggcga
     3661 atgcgttggg tccttccggg ccggtgatgc ccgagcgcgt gctgaccaag gcgccgaccg
     3721 cggagttgcg ccccaaccag accgaccagg attcgctgcc gccttatgaa atcctcgacg
     3781 gcatcctgga atgcctggtc gagaaggagc tcaccgtgga ggcgatcgtg gcgaagggtt
     3841 tcgaccgcgc cacggtgctg cgggtctggc gcatgctgga tctcgcggaa tacaagcggc
     3901 gccaggcgcc cccgggcgtg aagatcaccc atcgcgcctt cggcaaggac cggcggtatc
     3961 ccatcaccaa cggc
//
LOCUS       DASYCS010000435        11554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_711050,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000435 DASYCS010000000
VERSION     DASYCS010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 11554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11554
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711050"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..371)
                     /locus_tag="VGM46_13275"
     CDS             complement(132..371)
                     /locus_tag="VGM46_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006333070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HEY4193612.1"
                     /translation="MAKLVYKVVEHDGGWAYKVGSTFSETFPSHAQALHAAERASAEQ
                     QVSGTTDGIVYEDADGNWHEELADGHDRPQTEVSD"
     gene            complement(515..1618)
                     /locus_tag="VGM46_13280"
     CDS             complement(515..1618)
                     /locus_tag="VGM46_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HEY4193613.1"
                     /translation="MTASLFEPITLAGLTFPNRIAIAPMCQYSADDGSATDWHLQQWM
                     SYAMSGAGMVTVEMTNVERRARISHGCLGLYSDHNEAAARRALDAAKRVAAPGVKFGT
                     QLGHAGRKASWQRPWEGGEPLKSGQDPWPVVSASAIPYNEEAQVPHALTDDEILETIE
                     LFAQAARRADRAGFDFIELHAAHGYLIFQFLSPLSNKRTDRWGGSIENRSRFAVEVAK
                     AVKKAAPNLMLGARLSVKDWVDGGFDVEDAIEAAKALKAEGIAYICCSSGGNSPLQKM
                     PSSPGYQIHLAEAVRKGADIPTRAVGLIDDPRQAEAVVAEGRADMVALGRGFLADPRW
                     GWRAAAALGDTIHPAPQLARSVTTMQHWMKETL"
     gene            1781..1865
                     /locus_tag="VGM46_13285"
     tRNA            1781..1865
                     /locus_tag="VGM46_13285"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1815..1817,aa:Leu,seq:taa)
     gene            complement(2063..3229)
                     /locus_tag="VGM46_13290"
     CDS             complement(2063..3229)
                     /locus_tag="VGM46_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019857867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY4193614.1"
                     /translation="MRRKSFLILIAFASSLAGCAGSNTHDLLNKTVIDAPASTIGSTH
                     EIFVATTRKKATADPRQVFDGERSLTTSYARVDVTVPKTHQVGNIERAKGRAASDPAK
                     DFTARRVTSYANQQQFAKALGADVTLRGDRALVFVHGFNNGFDDGIYRLTQIAADTSY
                     PGTPVLFSWASGARLTDYVYDKESANAARDDLEATLRLIARTKVKSIDIIAHSMGTWV
                     TMEALRQLAITGDRDLNGKLGYVILASPDIDVDVFKKQMIRYGKPDKPFTVLLSGDDR
                     ALKLSSFISGSKPRVGDYNDAADLASYGVVVVDLTKTKSADRLNHAKFADNPILVQLL
                     GDRLRAPASLSDTDQPLNQQRVNDLGQGIGQAVGAVAEIVITTPFKVLTVVTGQ"
     gene            complement(3357..4451)
                     /locus_tag="VGM46_13295"
     CDS             complement(3357..4451)
                     /locus_tag="VGM46_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006333105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY4193615.1"
                     /translation="MRLKTLIPFALALLVAGCGTNRTDELLGSAIVTASVTQISGNHS
                     IFIATTRKRSDDPSKVFDGERSATLNFARVNVTVPATHQTGQIERKSRGKSDDPSKYF
                     MASEVVGYDTAPKFSKALNADIAARGGRVMLFVHGYNTGFDDAVYRLSQIVHDAGYPG
                     TPVLFSWASGASTTSYVYDKESASAARDQLEVTLRMLEQSGARRIDIVAHSMGTWVTM
                     ETLRQMAITGDRDLAGKLGDVVLASPDIDVDVFKSQMRRYGKPDKPFILLLSDDDRAL
                     RISGMIAGSRPRVGDYKNAADLADYGVTVVDLSNAKGNDTFNHTKFADNPALVKELGQ
                     RLRQDDGFASDRDVTDRIKQLSQPAGSGIM"
     gene            4670..5938
                     /gene="ilvA"
                     /locus_tag="VGM46_13300"
     CDS             4670..5938
                     /gene="ilvA"
                     /locus_tag="VGM46_13300"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HEY4193616.1"
                     /translation="MTSYIDSVSNAAAAVREIFPETPLQENDYLSKKFGARILLKRED
                     LTPVRSYKIRGAFNFFRKTLAAGNQAELFVCASAGNHAQGFAYVCRHFGKKGVVFMPV
                     TTPQQKIDKTRLFGGEFVEIRLIGDFFDDCYHAALAFTDEAGAHMVPPFDHRDIIEGQ
                     ATVAQEILAQMPGGRTPDMVMLPVGGGGLAAGVTHYFAERRASGDKDTRFVFCEPAGA
                     PSLKDSLVAGKRVKLAKIDSFVDGAAVGEIGRENFRLLKGFEPEAVHLIPENRLCATM
                     IEMLNVEGVVLEPAGALAIDALKDFARKEIKGKTIVCVVSGGNFDFERLPDVKERALR
                     FQRLKKYFIIRFPQRPGALRDFLEMLGPDDDIARFEYLKKSARNFGSVLIGIETKDHR
                     NFDQLIAKFASEGVQYQDITDNETLAGFIV"
     gene            complement(5963..6403)
                     /gene="gloA"
                     /locus_tag="VGM46_13305"
     CDS             complement(5963..6403)
                     /gene="gloA"
                     /locus_tag="VGM46_13305"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEY4193617.1"
                     /translation="MRYLHTMVRVADIDASLDFYCAKLGLKEVRRHENEQGRFTLIFL
                     AASEDEQTGIAAKAPLVELTYNWDPEEYRGGRNFGHLAYEVDDIYATCQNLMDKGVTI
                     NRPPRDGNMAFIKSPDGISIELLQKGPAKEKAEPWASMPNTGSW"
     gene            6627..7223
                     /locus_tag="VGM46_13310"
     CDS             6627..7223
                     /locus_tag="VGM46_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEY4193618.1"
                     /translation="MEDKASATRWNATVGEAVARDEAGETGDLVEVSGAIKWFDVAKG
                     YGFILPDEGSAGDILLHVTCLRKDGFQTALEGSRIVCLVRQGDRGMQAFRVLSMDVST
                     AIHPAEMQEPRTHVSVTPESGLERAMVKWFNRTKGFGFLSRGEGTEDIFVHMETLRRY
                     GVTELRPGQVVLVRFGRGDKGLMAAEIHPDMGTLPVSH"
     gene            7249..7809
                     /locus_tag="VGM46_13315"
     CDS             7249..7809
                     /locus_tag="VGM46_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006329067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEY4193619.1"
                     /translation="MTHRKWLPTGGFAVALAFAVFAFLQIAQSFADGQAMILPVDPAP
                     LVAVTDSGKHSFDIEIADDAAERSAGLMYRENMADDHGMLFVFGVPQQVAFWMKNTPM
                     PLDLLFIDEDGKVRAIRQGEPQSEAVIAPGETVRFVLELKSGTAARDGIKEGDIVKHP
                     IIGGQGDDQGMDDAPADGKASGGSGG"
     gene            7824..8591
                     /locus_tag="VGM46_13320"
     CDS             7824..8591
                     /locus_tag="VGM46_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY4193620.1"
                     /translation="MQFFNHRGFDLAYFDREPQSGSGDPVLMIHGFASSHFVNWVAPG
                     WFKVLNDAGYRAIAIDNRGHGVSSKSYISADYEPAEMASDAAALLDHLGIERAHVMGY
                     SMGARIAAFMALSHPDKVATLVLGGLGIGMVDGVGDWDPIADALLAKDAAGITHVRGK
                     MFRAFADQTHSDRQALAACIIGSRTLLGEEEVARIGQPTLIAVGTTDDIAGSPQELAA
                     LMPRAEAFSIEGRDHMLSVGDKTFKKRVLEFYAENPL"
     gene            complement(8688..9302)
                     /locus_tag="VGM46_13325"
     CDS             complement(8688..9302)
                     /locus_tag="VGM46_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008875563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193621.1"
                     /translation="MENEILIDSALMRELSALYAAPQRHYHGLAHIEALLALAREYRP
                     ELADPEAFDAAIWFHDAIYDSQTKDNEAQSAALARQKLDGRADAARIERITAMIEATA
                     THQLPQSSDAGFLRDTSLFLDMDLSILGAPPNVFDAYERAVRQEYAWVPEPAWIAGRG
                     AVLKNFSSQAHVFHTQEFRRRFETQARENIERSLGALEAKAKGE"
     gene            9430..10275
                     /gene="cysE"
                     /locus_tag="VGM46_13330"
     CDS             9430..10275
                     /gene="cysE"
                     /locus_tag="VGM46_13330"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEY4193622.1"
                     /translation="MNINIARTSTLQPMDPIWRSIRDEALEAVTRDPLLSAFLYSTIL
                     NQESLEDAVIHRLAERLAHQDIGSDLIRQTFKAMAVDDTNWATTVRVDIQAYYDRDPA
                     CDRFIMPVLYFKGFHAIQTHRLAHWLWNHGRKDFALYLQSRSSSVFQTDINPAAKIGK
                     GVFLDHATGLVVGQTAVIEDDVSILHGVTLGGTGKAGGDRHPKIRHGVLIGAGAKILG
                     NIEIGHCSKIAAGSVVLSPVPDNKTVAGVPARVVGESGCDHPARQMDQLLPSQSMDHV
                     VSFDI"
     gene            10398..10601
                     /locus_tag="VGM46_13335"
     CDS             10398..10601
                     /locus_tag="VGM46_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008875567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3126 family protein"
                     /protein_id="HEY4193623.1"
                     /translation="MKPEEIRKLDAYFKRVFQNANLEVKARPRKDDSAEVYVGDEFLG
                     LVFKDEDDGDYNFSMAILDIDLA"
     gene            complement(10641..11378)
                     /locus_tag="VGM46_13340"
     CDS             complement(10641..11378)
                     /locus_tag="VGM46_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193624.1"
                     /translation="MKQAALADSHIFAVPSSSFMMKLFYAFAALALLSAAISFSGKLF
                     GHAIAMGGYTDDGTLREVVIGNNVITIPANAIRFDRARHDGVASRLDLYLRYPALDGY
                     SEAARSDFNGQGAARNIIFLAFEEQMMSRDMSGRFAPIYSSLIEQPGLSGPDGTTLYN
                     FKAKTGYLNEVLVVGQRPGKDPFVARCLSGASAAQSLAPCERDIQVGEDLSLTYRFPQ
                     EFLANWQALDAAIAEKAAKILKTGRKR"
BASE COUNT         2207 a         3310 c         3708 g         2329 t
ORIGIN      
        1 atggttggcg gcaggcaatg tcgtatcctg cgcatcggac aatcgcagcg gacacgccat
       61 ggatttcgcc tgtccgcgct tatcttattg tactggcttg tctttaggtc attcagtccc
      121 gatgcgcggc gtcagtcgga gacttccgtc tgtggccggt cgtggccgtc ggccaattcc
      181 tcatgccagt tgccgtcggc atcctcatag acgatcccgt ccgtcgtccc cgagacctgc
      241 tgttccgcag atgccctttc ggcggcgtgc aaggcctgtg cgtggctagg gaaggtttcg
      301 gaaaaggtcg atccgacctt gtaggcccag ccgccatcgt gttcgacaac cttgtagaca
      361 agtttcgcca ttggaatctc cgggtttggt tttgagcgga cctgaccgtc aggtccgccc
      421 gccggcaaag ctttcaggaa cgcaaggcgc ggtcaatggt tgcggtatcg cctgtgcgcc
      481 tcgcagccat cgccaacagt tatttgcttg cggcctacag cgtctctttc atccaatgct
      541 gcatcgtggt gacggagcgg gccaattgcg gggccgggtg gatcgtgtcg cccagtgccg
      601 ctgccgcgcg ccatccccag cgcggatcgg ccagaaagcc gcgacccagc gcgaccatgt
      661 cggcccgacc ttcggccacc accgcctcgg cctgccgggg gtcgtcgatc agcccgaccg
      721 ctcgcgtcgg aatgtcggcg cccttgcgca cggcctcggc cagatggatt tgatagccgg
      781 ggctggaagg cattttctgc aagggagaat tgccgccgct ggaacagcat atatacgcga
      841 tgccttcagc cttcagcgcc ttggcggcct cgatggcatc ttcaacgtcg aagccaccat
      901 cgacccagtc tttcaccgat agtcgggcgc cgagcatgag gtttggagcg gctttcttca
      961 cggccttggc gacttcgacc gcgaaacggg agcggttttc gatggagccg ccccagcggt
     1021 ctgtacgctt gttggaaagc ggcgacagga actggaagat cagatagccg tgcgcggcgt
     1081 gcaactcgat gaagtcgaag ccggcgcggt cggcgcgccg ggcggcttgg gcgaagagtt
     1141 cgatggtttc caggatctcg tcgtccgtca gggcatgcgg aacctgcgct tcttcattat
     1201 agggaatggc ggaggccgag accaccggcc atgggtcctg tccggacttc agcggctcgc
     1261 cgccctccca cggacgctgc caggaagcct tgcgtccggc atggccaagt tgggtgccga
     1321 atttgacgcc gggtgctgcg acacgctttg ccgcatcgag cgcgcgccgc gccgccgctt
     1381 cattgtggtc ggaatagagg ccaaggcagc catgcgaaat acgcgcgcgc cgctcgacat
     1441 tggtcatctc gaccgtaacc atgccagcac cagacatggc gtagctcatc cattgctgga
     1501 ggtgccagtc ggtggcggag ccatcatcgg cggagtattg gcacatcggc gctatggcga
     1561 tgcggttggg aaaggtaagg ccggcgagcg taataggttc gaacaaagag gctgtcatga
     1621 aaggctccag aaagacacgc cctatgtagg gaccaagacc ggcattggcc agaccggcaa
     1681 catcacgaag gcgtggcggc agcgggccgc gccgcacggc attgtctgtc tgcataacgc
     1741 cggttgacgt tttggcgtaa agccttgaaa agcacgatgc gcggacgtgg cggaactggt
     1801 agacgcaagg gacttaaaat ccctcagcct cggctatacg ggttcgattc ccgtcgtccg
     1861 caccactcga attgccattg tcggaagatg ataaggggcg cgaaacagcc agcgggcatt
     1921 cccccctgga accacgcctc acgccggcaa atgctccgcg cccccatttg aaatctgtct
     1981 caattttcag gcgagcgcca atgaaacgat tgcttaacct taggcgctaa ccttaaccga
     2041 cgaggggcag gcatcctgca actcactggc cggtgacgac ggtcaacacc ttgaaaggtg
     2101 tggtgatgac gatctcggca acggccccga ccgcctggcc gatgccctgg ccaaggtcgt
     2161 tgacgcgctg ttggttcagg ggctggtccg tatcagacag gctggccggc gcgcgcaggc
     2221 ggtcgcccag caattgcacg aggatgggat tgtcggcgaa tttcgcgtgg tttagccggt
     2281 cggcgctctt cgtcttggtg agatcgacca cgacgacgcc atagctggca aggtcggcgg
     2341 cgtcgttata atcgccgacg cgaggcttcg atcccgagat gaagctggag agtttcaggg
     2401 cgcggtcgtc gcccgaaagc aggacggtga acggcttgtc cggcttgccg tagcggatca
     2461 tctgtttctt gaagacgtca acgtcgatgt cgggcgaggc gaggatgaca tagccaagct
     2521 tgccattcag gtcgcggtcg ccggtgatgg ccaattgcct cagcgcttcc atcgtcaccc
     2581 atgtgcccat ggaatgggcg atgatgtcga tgctcttgac cttggtcctg gcgatcagcc
     2641 tcagcgtggc ctcaagatcg tcgcgcgcgg cgttggcgct ttccttgtcg tagacatagt
     2701 cggtcagcct ggcgcccgaa gcccatgaaa acagcaccgg cgtgccgggg tagctggtgt
     2761 cggcggcaat ctgcgtcaac cggtagatgc catcgtcgaa gccattgttg aagccatgca
     2821 cgaagacgag cgcgcggtcg ccgcgcagcg ttacgtcggc gccaagcgct ttggcgaatt
     2881 gctgctggtt ggcataggac gttacccgcc ttgccgtaaa atccttggcg ggatcgctgg
     2941 ccgcgcggcc cttggcgcgc tcgatgttgc cgacctggtg cgtcttgggc accgttacgt
     3001 cgacacgagc gtagcttgtg gtgagtgagc gttcgccgtc gaatacctgg cgcgggtcgg
     3061 ccgtcgcttt cttgcgcgtc gtggccacga atatctcgtg ggtcgagccg atggtcgagg
     3121 cgggcgcgtc gataacggtc ttgttgagca ggtcatgggt gttgctgccg gcgcaaccgg
     3181 caagcgacga cgcgaaagcg atcaggatca agaatgactt gcggcgcacg ttaaggtcgc
     3241 tcctgaacaa ggtgcgtcct caacgccccg gccatggaat gatgcataac caattttggt
     3301 ccaactgcaa acgccatatg ccgggcttgc gccgaaaagg cctgtatcca aatttcctac
     3361 atgatgccgc tgccggccgg ctggctgagt tgcttgatgc ggtcggtcac atcgcggtcg
     3421 ctggcaaagc cgtcatcttg gcgaagccgc tggccgagtt ctttcaccag cgccggattg
     3481 tcggcgaatt tggtatggtt gaaggtgtcg ttgcccttgg cattggagag atcgacgacg
     3541 gtgacgccgt aatcggccag gtcggcggcg ttcttgtagt cgccgacgcg cggccgcgaa
     3601 ccggcgatca tgccggaaat ccgcaaggcg cggtcgtcgt cggacaaaag caggatgaac
     3661 ggcttgtccg gcttgccgta gcgccgcatt tggctcttga acacgtcgac atcgatatcc
     3721 ggtgaggcca gcaccacatc gccaagcttg ccggcaaggt cgcggtcgcc ggttatcgcc
     3781 atctgccgca acgtttccat cgtaacccat gtgcccatcg agtgggcgac gatatcgatg
     3841 cgccgcgcgc cggactgttc cagcatccgc agcgtcacct caagctggtc acgggcggcg
     3901 ctggcgcttt ccttgtcgta gacgtaactg gtggtcgaag cgccggaggc ccatgaaaac
     3961 agaacgggcg tgcccggata gccggcatcg tggacgatct gggagaggcg atagacggcg
     4021 tcgtcaaagc ccgtattgta gccatggacg aaaagcatga cgcggccgcc gcgcgcggcg
     4081 atatcggcgt tgagcgcctt ggaaaatttg ggcgcggtat cgtagccgac cacttccgag
     4141 gccatgaaat atttggaagg atcatccgac ttgccccgac ttttccgctc gatctggccg
     4201 gtctggtgcg tggccggaac ggtgacgttg acgcgggcaa aattcagagt ggccgagcgc
     4261 tcgccgtcga ataccttgct cgggtcgtcc gagcgcttgc gggtggtggc gatgaaaatc
     4321 gaatgattgc cggaaatctg ggttaccgaa gccgtgacga tggcgctgcc gagaagctcg
     4381 tcggtccggt tggtgccgca accggcaacc aacaaagcca gcgcgaaggg gataagggtc
     4441 ttcagacgca cgatcaaatc cagtcaaggc ccagggtgaa acgccggcaa aagcgagggg
     4501 cggctcgcgg ttcgttgcaa ttttgcgaaa gcttgtcccg ccagccaagt gcggcggaag
     4561 taagtcggag ccggctttac aggcaatcgg cgtctcagcg gaacgctgga ctgttgcacg
     4621 aaagccaaaa tggcggcagg aacgctgcga caagaatgga acccgcgcca tgaccagcta
     4681 tatcgacagc gtttcgaatg ccgccgccgc ggtgcgcgaa atattccccg aaacgccatt
     4741 gcaggagaac gactatcttt ccaagaagtt cggcgcgcga attctcctga agcgcgaaga
     4801 tttgacgccg gttaggtcct acaagatacg cggggcgttc aatttcttcc gcaagacgct
     4861 ggccgccggc aatcaggcgg aactgttcgt atgcgcttcg gccggcaacc acgcgcaggg
     4921 ctttgcctat gtctgccgcc atttcggcaa gaagggcgtc gtgttcatgc cggtgacgac
     4981 gccgcaacag aaaatagaca agacgcggct tttcggcggt gaattcgtcg agatacggct
     5041 gatcggcgat ttcttcgacg attgctacca cgcggctctc gccttcaccg acgaggccgg
     5101 cgcccacatg gtgccgccgt tcgatcatcg cgatatcatc gaagggcagg cgacggttgc
     5161 gcaggaaatc ctggcgcaga tgcccggcgg gcggacgccg gacatggtga tgctgccggt
     5221 cggcggcggt ggattggccg cgggcgtcac gcattatttc gccgaacgcc gcgccagcgg
     5281 cgacaaggac acgcgctttg tcttttgcga gccggcgggc gcgccaagcc tcaaggatag
     5341 tctcgttgcc ggcaagcgcg tgaaacttgc caagatcgat agtttcgtcg acggcgcggc
     5401 ggttggggaa atcggccgcg aaaatttccg cctgctcaaa ggcttcgagc cggaagcggt
     5461 gcatctcatt ccggaaaacc ggctttgcgc cacgatgatt gaaatgctga atgtcgaggg
     5521 cgtggtgctt gagccggcgg gcgcgctggc catcgatgcg ctgaaggatt ttgccaggaa
     5581 ggaaatcaag ggcaaaacca tcgtctgcgt ggtttcgggc ggcaatttcg attttgagcg
     5641 gctgcccgac gtcaaggagc gggcgcttcg cttccagcgg ctgaagaaat atttcatcat
     5701 ccgctttccg cagcggcccg gcgcgctgcg ggattttctc gaaatgctcg gccccgacga
     5761 cgatatcgcc cgcttcgaat atctgaagaa gtcagcgcgc aatttcggct cggtactgat
     5821 tggcatcgag acgaaggatc accgcaattt cgatcagctg attgcgaaat tcgccagcga
     5881 gggcgtgcaa tatcaggata ttaccgacaa tgagacgctg gccggcttca tcgtatgaac
     5941 gcgccgccgg ccgtgacgct gattaccagc ttcccgtatt gggcattgac gcccagggtt
     6001 cggccttttc cttggccggg cctttttgca aaagctcgat ggaaatgccg tccggcgatt
     6061 tgatgaaggc catgttgccg tcgcgaggcg ggcggttgat ggtgacgccc ttgtccatga
     6121 ggttctggca ggtggcgtag atgtcgtcga cctcataggc gagatggccg aaattgcggc
     6181 cgccgcgata ttcttccggg tcccaattat aggtcagttc gaccagcggc gctttcgcgg
     6241 cgatgccagt ctgctcgtct tcggatgcgg cgaggaatat cagcgtgaag cggccctgct
     6301 cgttttcgtg acggcgcact tccttgaggc cgagcttggc gcaatagaag tcgagcgaag
     6361 catcgatatc ggcgacgcga accatggtgt gcagatagcg catatgtctc tcctttggcg
     6421 ttgcggcgga caatagggag ggtgtggccg gctcgcaacc gcttcgatgc cggttcggtg
     6481 caagattggt cgtggcaaat aacgatggcg atattgttgt aaagagccgt gaaaaaagcc
     6541 ggcacgctct tgcacagacc tgaaatggcg gtgttaatct gtttgcaaga atcagttgcg
     6601 gtgttttcgg caaggggggc attccaatgg aagacaaagc ctctgcgacg aggtggaatg
     6661 cgaccgttgg cgaagccgtg gcgcgggacg aagcgggaga gacgggcgac cttgtcgaag
     6721 tctccggcgc gataaagtgg ttcgacgtgg ccaagggcta cgggttcatc cttcccgatg
     6781 agggatcggc cggcgatatc ctgctccatg tgacttgcct gcgcaaagac ggattccaga
     6841 ccgcgcttga aggttcgcgc attgtttgcc tggtcaggca gggcgatcgc gggatgcagg
     6901 cgttccgcgt cctgtcgatg gatgtctcga ccgccattca tcccgccgaa atgcaggagc
     6961 cgcgcacgca tgtgtcggtc acgcccgaga gcgggctgga gcgggcgatg gtaaagtggt
     7021 tcaaccgcac caagggcttt ggattcctga gccggggcga gggcaccgag gacattttcg
     7081 tccatatgga aacattgcgc cgctatggcg tgaccgagct tcggccgggg caggtcgtgc
     7141 tcgtgcgctt cggccgcggc gacaaaggcc ttatggccgc cgaaattcac cccgatatgg
     7201 gcaccttgcc ggtctcgcac tagcgcgagg ccggaccgag gaatgccgat gacccataga
     7261 aaatggttgc cgaccggcgg ctttgccgtg gcgctggcct ttgccgtatt tgcctttctc
     7321 cagatagcgc agagctttgc cgatgggcag gcgatgatcc tgccggtgga cccggcgccg
     7381 ctggtcgcgg taacggatag cggcaagcat tcgttcgaca tcgagatcgc cgacgacgcg
     7441 gcggagcgtt ccgccgggct aatgtaccgc gaaaacatgg ccgatgatca cggcatgctg
     7501 ttcgtgttcg gagtgccgca gcaggtggcg ttctggatga agaacacgcc gatgccgctc
     7561 gatctcctgt tcatcgatga ggacggcaag gtgcgtgcca tcaggcaggg agagccacaa
     7621 tcggaggcgg tgatagcgcc cggcgaaacg gtgcgcttcg tgcttgaact gaagtcgggg
     7681 actgccgcca gggacggcat caaggaaggc gatatcgtca agcatcccat aattggcggt
     7741 cagggcgacg accagggcat ggacgatgcg cctgccgatg gaaaagcttc gggcggcagc
     7801 ggcggctgac gggaatgcca ggcatgcaat ttttcaacca ccggggtttc gacctcgctt
     7861 atttcgaccg cgaacctcaa tccggcagcg gcgacccggt gctgatgatc catggctttg
     7921 catccagcca cttcgtcaac tgggtggctc cgggttggtt caaagtgctg aatgacgccg
     7981 gctaccgcgc gattgcgatc gacaatcgcg gccatggcgt ttccagcaag agctatatat
     8041 cggccgatta cgagccggcg gaaatggcgt ccgacgcggc ggcgctgctc gaccatctcg
     8101 gcatcgagcg cgcccatgtt atgggttatt cgatgggcgc gcgcatcgcc gctttcatgg
     8161 cgctgtccca tccggacaag gtcgcaacgc tggtgctggg cggccttggc atcggcatgg
     8221 tggatggcgt tggcgactgg gacccgatcg ccgatgcatt gctggccaag gatgccgccg
     8281 gcatcacgca tgtgcgcggg aaaatgtttc gcgctttcgc cgaccagacg catagcgacc
     8341 ggcaggcgtt ggccgcctgc atcatcggct cccgaacgtt gcttggtgaa gaagaggtgg
     8401 cgcgcatcgg ccagccgacg ctgattgccg tcggcaccac cgacgacata gccggctcgc
     8461 cgcaggaact tgcagcgctg atgcctaggg cggaagcatt ttccatcgag ggcagggatc
     8521 acatgctgtc ggtcggcgac aagacgttca agaagcgggt gttggaattc tacgccgaaa
     8581 acccgctctg acaggaccgg agttcaattg agcgcgccgg caaatggtga tttgcgcgct
     8641 tgcgctgctc caatgcgctg ttttcatttc gcgctgacct caaattctca ctcgcctttc
     8701 gccttggcct ccagcgcacc cagcgaacgc tcgatatttt cgcgcgcttg agtttcgaaa
     8761 cggcggcgaa attcctgcgt atgaaaaaca tgcgcttggc tgctgaaatt cttcaaaacc
     8821 gcgccgcgcc cggcaatcca ggcgggttcc ggcacccatg cgtattcctg gcgcacggca
     8881 cgctcatagg cgtcgaaaac attcggcggc gcgccgagga tggacaaatc catgtcgagg
     8941 aagagagaag tgtcacgcag aaagccggca tcggacgatt gaggcagttg gtgcgtggcg
     9001 gtggcctcga tcatcgccgt gatgcgttcg atccgagccg cgtctgcccg accgtccagc
     9061 ttttggcgcg caagggccgc actttgcgcc tcattatcct tggtctgact gtcgtagatg
     9121 gcgtcgtgga accagatcgc cgcgtcgaac gcttccggat cggccaattc cgggcggtat
     9181 tctcgcgcca gtgccagcag cgcctcgata tgggcaaggc cgtgatagtg ccgctggggc
     9241 gcggcataca gggctgagag ttcacgcatc agggcgctgt cgatcaagat ttcgttttcc
     9301 attgccggtt gaatttacgc cagcccgatc catttgacac tggcttaagg aaaattgagt
     9361 tcacccatgc tatcatctgg cttataggtg cggctcttaa atgctcaagc cggcaggcgg
     9421 agacgttcga tgaacatcaa tatcgctcgc acgagcacat tgcagccgat ggatccgatc
     9481 tggcgttcca tccgcgacga ggcgctggaa gcggtgacgc gcgacccgtt gctgtcggca
     9541 ttcctctatt cgaccatcct caatcaggaa agcctggaag acgcggtgat ccaccggctg
     9601 gccgagcggc ttgcccacca ggatatcggt tctgacctga tccgccagac attcaaggcg
     9661 atggccgtcg acgataccaa ctgggcgacg acagtgcgcg tcgacatcca ggcctattac
     9721 gaccgcgatc cggcttgcga ccggttcatt atgccggtgc tctacttcaa gggctttcac
     9781 gccattcaga cgcaccggct ggcccattgg ctgtggaatc atggccgtaa ggattttgcg
     9841 ctttatctgc aaagccggtc gtcgtcggtt ttccagacgg atatcaatcc ggcggcgaag
     9901 atcggcaagg gcgttttcct cgatcatgcg accggactgg ttgtcggcca gacggcggtg
     9961 atcgaggacg atgtatcgat cctgcatggg gtgacgctgg gcggtaccgg caaggcgggc
    10021 ggcgaccgcc acccgaagat ccgccacggc gtgcttatcg gcgcgggcgc caagatattg
    10081 ggcaatatcg agatcggcca ttgctcgaag atcgcggcgg ggtcggtggt gctgtcgccg
    10141 gtgccggaca acaagaccgt tgccggcgtg ccggcgcgcg tggtcggcga aagtggttgc
    10201 gatcatcccg cgcgccagat ggaccagctg ttgccgtcgc aatcgatgga tcacgtcgtc
    10261 agtttcgaca tctgattttg tttcggtatt taagcggtgg gctttggcat gaaggcggca
    10321 cgtcgccttt acatcgtcat ccctgccgtg ccagaagcgc cgtcacacca gcaagaccag
    10381 atggatcgga gaagacgttg aagccggaag aaatcagaaa actggacgcc tatttcaagc
    10441 gcgtttttca gaatgccaat ctggaagtga aggcgcgtcc gcgcaaggac gattcggccg
    10501 aagtgtatgt cggcgacgaa tttctcggcc tcgtcttcaa ggacgaagat gatggcgact
    10561 acaatttctc gatggctatc ctcgacatcg acctggcctg atccggaagg tcgattgctg
    10621 ccggtcgggc gttggaacgg ctatcgtttg cgcccggttt tcaggatttt ggcggctttt
    10681 tccgcaatgg cggcgtccag cgcttgccaa ttcgccaaaa actcctgcgg aaagcggtaa
    10741 gttaggctca aatcctcgcc gacctgaata tcccgttcgc atggcgccag cgattgcgcg
    10801 gcgctcgcgc cgctcaagca gcgggcgacg aagggatctt ttcccggacg ttggccgaca
    10861 accagcacct cgttgagata tccggttttt gccttgaagt tgtagagcgt agtgccgtcg
    10921 gggcctgaca ggcccggctg ctcgatcaag gaactgtaga tcggcgcaaa gcggccgctc
    10981 atgtcccggg acatcatctg ttcctcgaag gccaggaaga tgatgttcct ggccgctccc
    11041 tggccgttga aatcgctgcg cgcagcttcg ctgtaaccgt ccaacgccgg atagcgcagg
    11101 tagagatcga gccgcgaggc aacgccgtca tggcgcgcgc ggtcgaagcg tatggcgttg
    11161 gctgggatgg tgatgacatt gttaccgatt acgacctcgc ggagcgtacc gtcatcggta
    11221 tagccgccca tcgcgatggc gtggccaaac agtttcccgc taaagctgat cgccgccgaa
    11281 agcagggcca gcgcggcgaa ggcgtagaac agcttcatca tgaacgacga acttggcacg
    11341 gcgaatatgt ggctgtcggc taacgcggcc tgtttcatcg aaccctcgtt tacgcgtgcc
    11401 cgaatggcac acgcgacacg gcatgagcct tgcgtcgccc ggcatgactg tgctgttttg
    11461 tggtaaacgg aaggtaaata tgggcacgat agagatggcg gccttcctct ttctggtcgg
    11521 gatcaccgcc tccggcctcg tcggatctgt catg
//
LOCUS       DASYCS010000436         3011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4334981,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000436 DASYCS010000000
VERSION     DASYCS010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4334981"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /gene="purH"
                     /locus_tag="VGM46_13345"
     CDS             complement(<1..299)
                     /gene="purH"
                     /locus_tag="VGM46_13345"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912183.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEY4193625.1"
                     /translation="MAVASKMIPAPDLVPVRRALLSVSDKTGLVEFAQALAKAGVELV
                     STGGTAKAIAAAGIAVRDVSELTGFPEIMDGRVKTLHPSVHGALLGVRDDPEHSAA"
     gene            complement(392..2080)
                     /locus_tag="VGM46_13350"
     CDS             complement(392..2080)
                     /locus_tag="VGM46_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HEY4193626.1"
                     /translation="MLMAKEVWRRTRRRLRAGPVYRWRYSGRTPERVLIAPPDLRLAD
                     PQIALEIYYGRYPLSGHLVETGGQSPFQMEVASRGWQKSLHGFRWLRHMRAAGTELAA
                     ANARALVSDWIAMHGGRISGAAWEPGTTAKRIISWLQHSSVVLQGAEFPFYRAFLKSL
                     AVQIRYLRTVTPEMHNSKDRLRARIALAFAALSLPAPATALRAATRNLSEELDRQILP
                     DGGHISRNPMYVLEILADLLPLRQTYANQAETPPAALMGAIDRMLPALRFFRHQDGSI
                     ARFNGMGATIHDRILAILRHDDTIGSPLLHAPHSGYERLAMGGVTVIADTGAGPPVDV
                     SGAAHAGCLSFELSSGRQHYIVNAGIDTYGAAEFRPLARATAAHSTATLNDTSSARFS
                     YSARVSDVLGSPLTGGPKHVPCRRTDDKERQGFVARHDGYAARFGILHERELSLTGNG
                     NVLSGRDRFHEAGGGPIHSGGMDNVAIRFHVHPDVGLAQDENGRLVLTADNADRWVFT
                     STEIASQVEESIYFAGLGGPRRSRQIVLEFRASEIAEVHWQLIRTKIAGAPERN"
     gene            complement(2207..>3011)
                     /locus_tag="VGM46_13355"
     CDS             complement(2207..>3011)
                     /locus_tag="VGM46_13355"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEY4193627.1"
                     /translation="HRHEAPVDFSVKADPEIWAQKLGGIVTPAGTVRVAELDGAVAEQ
                     PGFAEGAWWVQDAAASLPARLLGDISGLRVADLCAAPGGKTAQLILAGARVTALDASK
                     NRLARLTQNLQRLGLAAEIAQADLLEYQPEALFDAVLLDAPCSSTGTVRRHPDVPWTK
                     TPADIEKLADLQRRLLTRAVMLVRPGGRIVFSNCSLDPLEGEELYRQFLADTPGVAND
                     PVLPGEILGVDPFLTAQGTLRTTPADMELGLPGISGLDGFFAARMRKAG"
BASE COUNT          538 a          999 c          990 g          484 t
ORIGIN      
        1 gccgccgaat gttcgggatc gtcgcgcacg ccaagcagcg cgccatgcac cgagggatgc
       61 agcgtcttga cccgcccgtc catgatttct ggaaagcccg tcagttcgga aacgtcccgc
      121 accgcgatgc ccgccgccgc aatcgccttg gcggtgccgc cggtcgagac aagctcgacg
      181 ccggccttgg ccagcgcctg cgcgaattcg accagccccg tcttgtcgga aaccgacagc
      241 aatgcgcggc gcaccggaac gaggtcggga gcagggatca ttttcgaggc gacggccatg
      301 gcgaatgcag cctttcctga cagggatttt tcccgttggg ccgtaacaca tggcgaccgg
      361 aaatcaaccc gcagcctatg cgcggcagcg atcagtttcg ctccggcgcg ccggcaatct
      421 tcgtgcggat caattgccaa tgcacctcgg cgatttccga cgccctgaac tccagcacga
      481 tctggcggct acggcgcggc ccgcccagcc cggcgaaata gatggattct tcgacctgcg
      541 acgcaatctc cgtcgaagtg aacacccagc ggtccgcatt gtccgccgtc agcaccagcc
      601 ggccgttctc gtcctgcgcc aggccgacgt ccggatggac atgaaagcgg atggccacat
      661 tatccatgcc gccgctatgg atcggcccgc cgcccgcctc atggaaacgg tcgcggccag
      721 acaggacatt gccattgccg gtcagcgaca attcgcgctc atgcaagatg ccgaaacgcg
      781 ccgcatagcc gtcatggcgc gcgacaaaac cctggcgctc cttgtcgtcg gtgcgccggc
      841 aaggcacatg cttcggcccg ccggtcagcg gcgagccgag cacgtcgctg acgcgggcgg
      901 aatagctgaa gcgggccgac gacgtgtcgt tgagcgttgc ggtcgaatgg gcggcggtgg
      961 cgcgcgccaa cggccggaac tcggccgcgc cataagtgtc gataccggca ttgacgatgt
     1021 aatgctggcg gccagacgac agctcgaagg acaggcaacc ggcatgcgcg gcgcccgaaa
     1081 cgtcgaccgg cggccccgcg cctgtatcgg cgatgacggt gacgccgccc atggccagcc
     1141 gttcgtagcc cgaatgcggc gcatgcagca gcggcgagcc gatggtgtcg tcatggcgca
     1201 atatggccag gatgcggtca tggatggtcg cacccatgcc gttgaagcgg gcaatgctgc
     1261 cgtcctgatg ccggaagaac cgcagcgccg gcagcatcct gtcgatagcg cccatcaggg
     1321 ccgcgggcgg cgtttcggcc tggttggcat aggtctggcg cagcggcagc aggtcggcca
     1381 ggatttcgag gacatacatc ggattgcgcg aaatatggcc gccatccggc aggatttgcc
     1441 ggtcgagttc ctcgctcaga ttgcgcgtgg cggcgcgtag cgcggttgcc ggcgccggca
     1501 atgaaagcgc cgcgaaggcc agcgcgatgc gcgcgcgcag ccgatccttg ctgttgtgca
     1561 tttcaggcgt caccgtgcgc agatagcgga tctgcacggc cagcgatttc aggaaggcgc
     1621 gatagaaggg aaattccgcg ccctgcaaaa ccacggacga atgctgtagc cacgagatga
     1681 tgcgcttggc cgtcgtgccg ggttcccagg cggcgcccga gatgcggccg ccatgcatgg
     1741 cgatccagtc ggacaccagc gcacgcgcat tggcggcggc aagctcggtg ccggcggcgc
     1801 gcatatggcg cagccagcgg aatccgtgca ggcttttttg ccagccgcgg ctggcgactt
     1861 ccatctggaa cggcgactgg ccgccggtct cgaccagatg gccggacagg gggtagcgcc
     1921 cataataaat ctcaagcgcg atctgcgggt cggcaagccg caaatccggc ggcgcgatca
     1981 gcacccgttc cggcgtgcgg cccgaataac gccagcgata gacaggcccg gcgcgcaggc
     2041 gacggcgcgt cctgcgccag acctccttgg ccatcagcat ccatacatgc gtcgtgtttg
     2101 tggcctctgc tgccaatacg ccctcggtaa cgttcttgtg gaagaacaac ggtagttgat
     2161 tcaagagctt atgcgaaggc ggtttggcct aatgcccacc gcttccttag ccggccttgc
     2221 gcatgcgcgc ggcaaagaag ccatcgaggc cggatatgcc gggcaagccc agctccatgt
     2281 ccgccggtgt cgtgcgcaac gtgccctgcg ccgtcaggaa ggggtcgacg cccaagattt
     2341 cacccggcag caccggatcg ttggcgacgc cgggcgtatc ggccaggaat tgcctgtaga
     2401 gttcctcgcc ctccagcgga tcgagcgagc agttggaaaa cacgatccgc ccgccgggcc
     2461 gcaccagcat caccgcgcgg gtaagcagcc ttcgctgcaa atcggccagc ttttcgatgt
     2521 cggccggggt cttcgtccac ggcacgtcgg ggtgacggcg caccgtgccg gtggaggagc
     2581 atggcgcatc cagcagcacc gcatcgaaca gtgcttccgg ctgatattcg agcaggtcgg
     2641 cctgggcgat ttccgcagcc agaccgaggc gctggagatt ctgggtcagc cgcgccagcc
     2701 ggttcttcga cgcgtcgagc gccgtgaccc tggcgccggc caggatcaat tgagcggtct
     2761 tgccgccggg cgccgcacac aggtcggcga cgcgaagccc gctaatgtcg cccagcagcc
     2821 gggcaggcag gctggcagcg gcatcctgca cccaccacgc gccttcggca aagcccggct
     2881 gttcggcaac cgcgccgtca agttccgcca cccgtaccgt gccggccggc gtgacgatgc
     2941 cgcccagttt ttgcgcccag atttcaggat cggccttgac cgaaaaatcc accggcgctt
     3001 catgccggtg c
//
LOCUS       DASYCS010000437         3240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1438503,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000437 DASYCS010000000
VERSION     DASYCS010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1438503"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="VGM46_13360"
     CDS             complement(<1..680)
                     /locus_tag="VGM46_13360"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018430572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEY4193628.1"
                     /translation="MSDTLNVLEASLLADIAGAADEPSIEAVRVAALGKKGSISELLK
                     TLGAMTPEERQTKGPAINGLKNRITEALTQRKGALKDIAITARLAAEKVDVTLPVRQS
                     PAERGRIHPISQVIDEIAAIFGDMGFAIAEGPDIETDHYNFTALNFPEGHPAREMHDT
                     FFFQPDEKGERKVLRTHTSPVQIRTMETQKPPIRIVIPGKTYRQDSDATHSPMFHQVE
                     GLVIDKTA"
     gene            complement(964..1365)
                     /gene="rplT"
                     /locus_tag="VGM46_13365"
     CDS             complement(964..1365)
                     /gene="rplT"
                     /locus_tag="VGM46_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006201247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEY4193629.1"
                     /translation="MARVKRGVTSHAKHKKVIKAAKGFYGRRKNTIRIAKQAVEKSLQ
                     YAYRDRKVRKRSFRALWIQRINAATHEHGLTYGRFIDGLNKAGIEIDRKVLSDMAIHE
                     PQAFAALVAKAKVALEYLKNTTPNAFESAVA"
     gene            complement(1453..1656)
                     /gene="rpmI"
                     /locus_tag="VGM46_13370"
     CDS             complement(1453..1656)
                     /gene="rpmI"
                     /locus_tag="VGM46_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEY4193630.1"
                     /translation="MPKMKTKSAAKKRFKITGTGKVLSAAAGKRHGMIKRSNKFIRNA
                     RGTMVLAEPDGKKVIKNFLPNGL"
     gene            complement(1839..2465)
                     /locus_tag="VGM46_13375"
     CDS             complement(1839..2465)
                     /locus_tag="VGM46_13375"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013891665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEY4193631.1"
                     /translation="MDDEARESLGAYQSMRRLLLAITVVVMVAALLFGQSIFTPETPV
                     HEAIEMFGILLIFLGIVGRLWSTLYIGGRKSAEVVTGGPYSVTRNPLYVFSSVAAAGV
                     GAQMGSIVAMIGFGVLCAAAFHIVILREEKFLTGAMGESYRAYLTKVPRFFPKLSLYQ
                     EGDTGSFKPRLLLNTLLDGLIFLVAMPVFETIDAAQLSGTLPVLFRLP"
     gene            complement(2589..3125)
                     /gene="infC"
                     /locus_tag="VGM46_13380"
     CDS             complement(2589..3125)
                     /gene="infC"
                     /locus_tag="VGM46_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEY4193632.1"
                     /translation="MRRPFKAAAPTKDGPRANTDIRVPRVQLIGVDGENRGEVSINDA
                     LLLAEEAGLDLVEISPNANPPVAKILDLGKLKYASQKKAAEARKNQKVIEIKEIKMRP
                     NIDSHDYETKMKAVRRFFDEGDKVKLTLRFRGREMAHMELGMQLLNKVREETATIAKV
                     EAEPKLEGRQMMMVLAPR"
BASE COUNT          590 a          944 c         1000 g          706 t
ORIGIN      
        1 ttggccgtct tgtcgataac cagcccctcg acctgatgaa acatcggcga gtgggtggcg
       61 tcgctgtcct gccggtaggt cttgcccgga atgacgatgc ggatcggcgg cttttgcgtt
      121 tccatggtgc gtatctgcac aggcgaggtg tgggtgcgca gcaccttgcg ctcgcccttc
      181 tcgtccggct ggaaaaagaa agtatcgtgc atttcgcgcg ccgggtggcc ctcggggaaa
      241 ttcagcgcgg tgaaattata gtggtcggtc tcgatgtccg gcccttcggc gatggcaaaa
      301 cccatgtcgc cgaagatcgc agcgatctcg tcgatgacct ggctgatcgg gtggatgcgg
      361 ccgcgctcgg ccggcgactg gcgcaccggc agcgtcacgt cgaccttttc ggcggcaagc
      421 cgcgcggtga tggcgatgtc cttcaacgcg cccttgcgct gcgtcagcgc ttcggtgatg
      481 cggtttttga ggccgttgat cgccggaccc ttggtctggc gctcctcggg cgtcattgcg
      541 cccagggttt tcagcagttc cgagatcgag cccttcttgc cgagcgccgc cacccgcacc
      601 gcttcgatgg acggttcgtc ggcggccccg gcaatgtcgg cgaggaggga tgcttcaagg
      661 acgttcagtg tatcgctcac ggtcgcttcc gttcgttgcc gtcatgcccc ctccccggct
      721 cgccgcgcca ccagcgaggc tgcggctctt ttcccgaggg aaaagctggc ccgacgggcc
      781 ggatgagggg cagttgcggc tgccagtgtt cagtcacaag aagtgttcag tcacaacaaa
      841 aacccgcgcc agccgtgcca gcgcgggttt cccaaatcaa gtgtcgatgc ttgggaaggc
      901 gctgggcttt ccttatcgca tttgtccgac gcgggaattg ctgcgcaacc cgctggaaaa
      961 tgcttaggct acagcgcttt caaaagcgtt cggcgtggtg ttcttcagat attcgagggc
     1021 gaccttggcc ttggccacaa gcgcggcgaa tgcctgcggc tcgtggatgg ccatgtcgga
     1081 caggaccttg cggtcgattt cgatgcccgc cttgttgagg ccgtcgatga agcggccata
     1141 ggtcaggcca tgctcgtgcg tcgccgcgtt gatgcgctgg atccacaatg cgcggaagga
     1201 gcgcttgcgg accttgcggt cgcggtaggc atattgcagc gacttctcga ccgcctgctt
     1261 ggcgatgcgg atggtgttct tgcggcggcc ataaaagccc ttggcggctt tgatgacctt
     1321 cttgtgcttg gcgtgcgagg tgacgcctct ttttacgcgt gccatgtcat gatctcctta
     1381 aaactgtcca tttgttcgcc tgacatcgtc cgaaaagcct gcaacttttc gggatcaggc
     1441 ttgccgattg ccttagaggc cgttcggcag aaaattcttg atgaccttct tgccatccgg
     1501 ttcagccaga accatcgtgc cgcgggcatt tcgaatgaac ttgttggaac gcttgatcat
     1561 gccgtgacgc ttgccggccg cagccgacag gactttaccc gtacctgtga tcttgaaccg
     1621 cttcttggcg gccgatttgg tcttcatctt gggcattttg ctatcctttt ttggtgcgca
     1681 cgattgcgct tgtttgttgc ttcaggccga ccaaagcccg aaaagcaaat gaaaccgcca
     1741 cggcatgccc tgccgggcgg tttttcagga acggcgggtc tatacggcaa acgatcgcgg
     1801 cgcgcaacac ccgtccggcg ccgcggtgac cggccctctc agggaagccg gaacagcacc
     1861 ggcagcgtcc ccgacagctg agcggcatct attgtttcga ataccggcat cgccaccagg
     1921 aagatcagcc cgtcgagcag ggtgttgagc agcaggcggg gcttgaagct gccggtgtcg
     1981 ccttcctgat agagagaaag cttgggaaag aagcgcggca ctttcgtaag ataggcccgg
     2041 tagctctcgc ccatcgcgcc agtcaggaat ttttcctcac gcaagatgac gatgtggaac
     2101 gccgccgcgc acaggacgcc gaaaccgatc atggcgacga tcgatcccat ctgcgcgccg
     2161 acgccggcgg cggccaccga agaaaacacg taaagcggat tgcgggtgac cgaatagggt
     2221 ccgccggtga cgacctccgc cgatttacgc ccgccaatgt aaagcgtcga ccacagacga
     2281 ccgacgatgc cgaggaagat cagcaggatg ccgaacattt cgatggcttc gtgaaccggc
     2341 gtctcggggg tgaagatcga ctggccgaac aacaacgcgg caaccatgac caccaccgtg
     2401 atggcgagca gcagccggcg catgctttgg taggcgccga gcgattctct ggcttcatca
     2461 tccatgggga aagtccgatc gtcgaaatgc ttggctatgc ctgccggcag tcgcttcgat
     2521 aaacagcagg aaaagcaaaa acaggcacct tgctgcggat gcctgttcaa ttggggatgg
     2581 agaaagggct agcgcggggc cagcaccatc atcatctggc ggccttcgag ctttggctcg
     2641 gcctcgacct tggcgatggt cgccgtttct tcgcgcacct tgttcaaaag ctgcatgcca
     2701 agttccatat gcgccatctc gcggccgcga aagcgcagtg tcagcttgac cttgtcgccc
     2761 tcgtcgaaga accgccgcac cgccttcatc ttggtctcgt agtcgtgact gtcgatgttc
     2821 gggcgcatct tgatctcctt gatctcgatg accttctggt tcttgcgggc ttcggcggcc
     2881 tttttctggc tggcgtattt caacttgccg agatcgagaa tcttggcaac cggcggattg
     2941 gcgttgggcg aaatttcgac aagatcgagc ccggcctctt cggcgagcag caatgcgtcg
     3001 ttgatggaaa cttcaccgcg attctcaccg tcaactccga tgagctggac gcggggaacc
     3061 cggatatcgg tattagcgcg cggcccgtcc ttggtaggcg cggctgcttt aaagggtctg
     3121 cgaatggtcg tggtctcctt ggccgtttcg ttcaggattt cgaatgcgag aatgtggacg
     3181 cgccaatgtg gtaagcgcgc ggtcgaagtc aatagcacag gacttaacgg aaatcaccag
//
LOCUS       DASYCS010000438         2587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_5206144,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000438 DASYCS010000000
VERSION     DASYCS010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5206144"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="VGM46_13385"
     CDS             <1..397
                     /locus_tag="VGM46_13385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193633.1"
                     /translation="FAAMKQPDMRRFPSLEAIPSGEAISADNMATPSIIAMGEPAPGI
                     GYEQVAAIPKPKRGPLYQPMVIRGGVAGDAFAPASSSDQPAVATSAPARSPANPSPAG
                     PASGGQPPSAPPPPTPTAPPVLAPKGMPE"
     gene            complement(505..>2587)
                     /gene="glyS"
                     /locus_tag="VGM46_13390"
     CDS             complement(505..>2587)
                     /gene="glyS"
                     /locus_tag="VGM46_13390"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018689420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEY4193634.1"
                     /translation="ETEEPVVVDFEVDGIRSGNVTYGHRFHAPGEITVRRFEDYAGKL
                     EAAKVVLDADRRKEIILADAKNLAFANGLDLVEDEGLLDEVAGLVEWPVVLMGEFEQD
                     FLSIPAEVIRLTIRANQKCFVTRPQGESESLSNRFILTANIEAKDGGKEIAHGNGKVV
                     RARLSDAVHFWKTDQADLPDLGALEKSAEKFGLDLKKPLDQRMARLDHLGVTFHAKLG
                     TQGERVERIRRLAREIAPIVRADPDMAERAAILAKADLTTEIVGEFPELQGTVGRKYA
                     LLQGEDVSVAAAIEDHYKPQGPSDRVPTDPVSIAVALADKLDTLVGFWAIDEKPTGSK
                     DPYALRRAALGVVRILVENGVRVDLSVTIAAHFLNQLMGYMSEIASMVAMSDNDKNQE
                     EDFSWDLAGKKSERLLRKAFELFLLNHDGMQGTAEETYRASFDEWDISVPDREKREEF
                     LSKRWKVYEESIRTRFEWSGHENDRAIESARKIPGAFTEEVRQVLRNGKVSEFVFGYP
                     VVAAKFLASDLLSFFHDRLKVYLRDQGARHDLIDAVLAAGGASPSNDDLLIIVRKVEA
                     LGQFLDTEDGKNLLAGAKRAANILAAEEKKKTAIAAVVDPSLFRDEPEKNLFAAVNQA
                     ESQAAQAIQNEDFSAAMLALSVLREPVDSFFEHVHVNDEDAAVRANRLALLARIRAAT
                     DRVADFSKIAG"
BASE COUNT          500 a          827 c          736 g          524 t
ORIGIN      
        1 attcgcggcc atgaagcaac cggatatgcg ccgttttcca tcgctggaag cgataccatc
       61 cggggaagcg atctcggccg acaacatggc aacgccgtcg atcatcgcca tgggcgaacc
      121 cgcgccgggg ataggatacg aacaggtagc ggccattcca aaaccaaagc gcggacccct
      181 gtaccagccg atggtcattc gcggcggcgt cgcgggcgac gccttcgcac ccgcatcgag
      241 cagcgaccag ccggcggtcg ccaccagcgc gcccgcccgc tccccggcaa acccctcgcc
      301 tgccggccca gcatcgggcg gccagccgcc atcggctccg ccaccgccga cgcccacggc
      361 accgccggtg ctggcgccca agggcatgcc cgaataggcg ttagagcggc tctggttttt
      421 accgaatcgg taaaaccgct ctatcttttt gttttagccg catttccgga cgcaaaaccg
      481 tttcacaatt ttgctggaaa tgctctagcc ggcgattttc gagaaatcgg caacccggtc
      541 ggttgcggcg cggatacgcg ccagcaaggc caggcggttc gcacgcacgg cggcgtcttc
      601 atcattcaca tgaacatgct caaagaatga atcaaccggt tcgcgcaaca cgctaagcgc
      661 cagcatggcg gcggaaaagt cttcgttctg aatcgcttgc gccgcctggc tctcagcttg
      721 attcaccgcg gcaaacaggt ttttctccgg ctcgtcgcgg aaaagcgatg gatcgaccac
      781 agccgcgatg gcggttttct tcttttcctc ggccgccaga atgttggccg cgcgcttggc
      841 gccagcaagc aggttcttgc cgtcctcggt gtcgaggaat tggccgagcg cttcgacctt
      901 gcgaacgatg atgagcaggt cgtcgttgga gggtgaagct ccgcccgccg ccaacacggc
      961 atcgatcaga tcgtgccgcg caccttggtc gcggagatag actttcaggc gatcgtggaa
     1021 gaaggagagg aggtcggatg ccaagaactt ggccgcaacc acgggatacc cgaaaacgaa
     1081 ttcgctcacc tttccgttgc gcaagacctg acgaacttcc tcggtgaatg cccccggaat
     1141 ttttcgtgca ctctcaattg ctcgatcatt ctcatgaccc gaccattcaa aacgagtgcg
     1201 aatactctct tcatacactt tccatctttt tgacagaaac tcttcccgct tctctcgatc
     1261 cggaaccgaa atatcccact cgtcgaagct agctcgatag gtctcttcgg ctgtgccctg
     1321 catcccgtcg tgattaagaa gaaatagttc aaatgccttt ctcaaaaggc gttcagactt
     1381 ctttccggca aggtcccacg aaaaatcctc ttcctgattt ttgtcattgt ctgacatcgc
     1441 gaccatggat gctatctcgg acatgtaccc cattagctga tttaagaaat gagccgcgat
     1501 tgtcacagag aggtcaaccc tcaccccatt ctcaaccagt atccgcacca cccccagcgc
     1561 cgcccgcctc agcgcataag ggtccttgct gcccgtcggc ttctcgtcaa tcgcccagaa
     1621 gccgaccagc gtatcgagtt tgtcggccag cgcgacggct atcgacaccg ggtcggtcgg
     1681 cacgcggtcg gaggggcctt gcggtttgta gtgatcttcg atggcggcgg cgacggaaac
     1741 gtcctcgcct tgcagcaggg cgtatttgcg gcccacagtg ccttgcaatt cggggaactc
     1801 gccgacgatt tccgtggtca ggtcggcctt ggccaatata gcggcgcgct cggccatatc
     1861 gggatcggca cggacaatcg gcgcgatttc gcgcgccagc cggcggatgc gctccacgcg
     1921 ctcgccctgc gtgccgagct tggcatggaa ggtgacgcca agatgatcga ggcgggccat
     1981 gcgctggtcg agcggctttt tcaggtcgag gccgaacttt tccgccgatt tttcgagcgc
     2041 gccaaggtcc ggtaaatcgg cctgatcggt cttccagaaa tgcacggcgt ccgaaaggcg
     2101 ggcgcgcact accttgccat tgccatgcgc gatttccttg ccgccatcct tcgcctcgat
     2161 attggcggtc aggatgaaac ggttcgacag gctctcggac tcgccctgcg ggcgcgtgac
     2221 aaagcacttc tggttggcgc ggatggtcag ccggatgact tcggcgggga tcgacaggaa
     2281 gtcctgctcg aactcgccca tcagcaccac cggccattcg accagccccg ctacctcatc
     2341 cagcaggcct tcatcctcga ccaggtcgag gccgttggca aaggcgaggt tcttggcgtc
     2401 ggcgagaata atttccttgc ggcggtcggc atcgagcacg accttggccg cctccagctt
     2461 gccggcataa tcctcgaagc gccgcacggt gatttcgccc ggcgcatgga agcggtggcc
     2521 ataggtgacg ttgcctgagc ggatgccgtc gacctcgaaa tcgaccacca ccggctcctc
     2581 ggtttcg
//
LOCUS       DASYCS010000439         4481 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1870580,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000439 DASYCS010000000
VERSION     DASYCS010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4481
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1870580"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="VGM46_13395"
     CDS             complement(<1..343)
                     /locus_tag="VGM46_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEY4193635.1"
                     /translation="MAKAATPQKAPAKAAAATKAAAPAKAAAAKAAPAKAVAAKSPAS
                     AVSKGVAAGKVRQVIGAVVDVQFAEGHLPAILNALETENVGNRLVLEVAQHLGENTVR
                     TIAMDSTEGLVR"
     gene            complement(420..1307)
                     /locus_tag="VGM46_13400"
     CDS             complement(420..1307)
                     /locus_tag="VGM46_13400"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006132906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEY4193636.1"
                     /translation="MASLKDLRNRIASVKATQKITKAMQMVAAAKLRRAQEAAEAARP
                     YSERMGAVLANIAQAVGGGGGDAPALMTGTSRDDTHLLVVCTAERGLCGSFNSQIARL
                     ARDHARQLLADGKEVKIICVGKKGYDILRRDFASQIIDRVDLREVKTLGFVNADAIAK
                     KIISLFNEGAFDICTLFYSHFRSVISQVPTAQQIIPAAVATTTGNDEANGGAIYEYEP
                     EPGEILADLIPRNISVQVFHALLENAAGEMGAKMSAMDNATRNAGDMINKLSITYNRQ
                     RQAQITKELIEIISGAEAL"
     gene            complement(1339..2868)
                     /gene="atpA"
                     /locus_tag="VGM46_13405"
     CDS             complement(1339..2868)
                     /gene="atpA"
                     /locus_tag="VGM46_13405"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEY4193637.1"
                     /translation="MDIRAAEISAILKDQIKNFGKEAEVSEVGQVLSVGDGIARVYGL
                     DNVQAGEMVEFPGGIRGMALNLEADNVGVVIFGADRDIKEGDVVKRTGAIVDAPIGMG
                     LLGRVVDALGNPIDGKGPIKYTERKRVDVKAPGIIPRKSVNEPMSTGLKAIDALIPVG
                     RGQRELVIGDRQTGKTAIILDAMLNQKSVHDNGPDAEKLYCVYVAVGQKRSTVAQFVK
                     VLEERGALEYSIIVAATASDPAPMQFLAPFTACTMGEYFRDNGMHALISYDDLSKQAV
                     AYRQMSLLLRRPPGREAYPGDVFYLHSRLLERAAKLNDDNGNGSLTALPIIETQANDV
                     SAYIPTNVISITDGQIFLETNLFFQGIRPAVNVGLSVSRVGSAAQIKAMKQVAGSIKG
                     ELAQYREMAAFAQFGSDLDASTQRLLNRGARLTELLKQPQFSPLKVEEQVAVIFAGVN
                     GYLDKLALKDVGRFEQGLLSHMRAAGKAILDQIRKDKALSDDLRAKLKAEIDAFAKTF
                     A"
     gene            complement(2868..3428)
                     /locus_tag="VGM46_13410"
     CDS             complement(2868..3428)
                     /locus_tag="VGM46_13410"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEY4193638.1"
                     /translation="MAQSSSPISGVAERYAGSLFDLASQANSVAKVEADLTSFETMLA
                     NSADLERLIKSPVFSSEDQAKAIAAVADKAGITGLAGNFLRVVAANRRLFAVPGMIKA
                     FRRIAAEHRGETSAEVTSAHELTAAQQTELKATLKSVAGKDVAITVTVDPSLLGGLVV
                     KMGSRQIDTSLKTKLNSLKLALKEVG"
     gene            complement(3674..4069)
                     /locus_tag="VGM46_13415"
     CDS             complement(3674..4069)
                     /locus_tag="VGM46_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006327477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4345 family protein"
                     /protein_id="HEY4193639.1"
                     /translation="MEYSFPWPMSQGEWLAWSSAVVTVLLGLLLFLAPGISFRVLRLQ
                     AKPEKPAAIAEARGRMSGFYLGVGLCCILLAQPLLYMALGFSWLFTAFGRLLSMMSDR
                     ANTPYNWASAAVELILAALPLAFAFGFLP"
     gene            complement(4186..>4481)
                     /locus_tag="VGM46_13420"
     CDS             complement(4186..>4481)
                     /locus_tag="VGM46_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEY4193640.1"
                     /translation="PPFGRLAGIIVSAASRAEAEGHARGLRRAAPEATDIFVLGPAEA
                     PLALLGGRHRFRLLIQGEKRADMQGFVRTLLAAGPKVRGSVRVQVDIDPQSFL"
BASE COUNT          786 a         1385 c         1437 g          873 t
ORIGIN      
        1 cacgcaccag gccctcggtg gagtccatgg cgatcgtgcg cacggtgttt tcgccgagat
       61 gctgggccac ttcgagaacg aggcggttgc cgacattctc ggtttcaagc gcgttcaaga
      121 tggcgggcag gtggccttcg gcgaactgca cgtcgacaac ggcgccaatg acctggcgaa
      181 ccttgccggc ggccacgcct ttgctgacgg cggatgccgg gcttttggcc gcaacggcct
      241 ttgccggagc cgccttggca gccgccgcct tggccggtgc tgccgctttc gttgccgctg
      301 ccgccttggc gggtgctttc tgcggggtcg ctgctttcgc catcgtgttt acccttttcg
      361 tccgtgttct tgtcctgcac gattttccag gaaggttttc ggccttcctc aaaccgtggc
      421 tagagcgcct cggcgcccga aatgatttcg atcagttctt tggtgatctg cgcctgccgc
      481 tgacggttat acgtgatcga cagcttgttg atcatgtcgc cggcattgcg cgttgcattg
      541 tccatggcgc tcatcttggc gcccatttcg cctgccgcgt tttcgagcag cgcatggaaa
      601 acctgcaccg aaatgttgcg cgggatgaga tcggccagga tttcgcccgg ctccggctcg
      661 tattcgtaaa tagcgccgcc attggcctcg tcattgccgg tggtcgtggc gacagcggcg
      721 ggaatgatct gctgcgcggt cggaacctgg ctgatgaccg agcggaaatg cgaataaaac
      781 agggtgcaga tatcgaaggc gccctcgttg aagaggctga tgatcttctt ggcgatggcg
      841 tcggcattga cgaagccgag cgtcttgacc tcgcgcaggt cgacacggtc gataatctgc
      901 gaagcgaaat cgcggcgaag gatgtcgtag cccttcttgc cgacgcagat gatcttcact
      961 tccttgccgt cggcaagcag ctgtcgggca tggtcacggg ccaggcgggc gatctgcgag
     1021 ttgaagctgc cgcacaggcc gcgttcggcc gtgcagacga caagcaggtg cgtgtcgtcg
     1081 cggctggtgc cggtcatcag cgccggggcg tcgccaccac cgccgccaac cgcctgcgcg
     1141 atattggcca gcaccgcgcc catgcgctcg gaatagggac gtgccgcttc cgccgcctcc
     1201 tgcgcgcgcc gaagcttcgc cgcggcgacc atctgcatcg ccttggtgat cttctgcgtc
     1261 gccttgacgg aggcgatacg gttacgaagg tctttcagtg acgccatgct tcaaactcgg
     1321 ttcccgcgtg cgtcctgttc aagcaaacgt cttggcgaag gcatcgatct ccgccttgag
     1381 cttcgcgcgc aaatcgtcgg acagcgcctt atccttgcgg atctggtcga ggatggcctt
     1441 gccggcggca cgcatgtgcg acagcaggcc ctgttcgaac cggccgacat ccttcagcgc
     1501 cagcttgtcg agatagccgt tgacgccggc gaagatcacc gcgacctgct cctcgacctt
     1561 gagcggcgag aactgcggct gcttcaggag ttcggtcagg cgtgcgccac ggttcagcag
     1621 gcgctgcgtc gaggcgtcga ggtcggaacc gaactgcgcg aaggccgcca tttcgcggta
     1681 ctgcgcaagc tcgcccttga tcgagccggc aacctgcttc atcgccttga tctgggcggc
     1741 ggagccgacg cgcgagaccg acaggccgac gttgaccgcc gggcggatgc cctggaagaa
     1801 caggttcgtc tcaaggaata tctggccgtc ggtgatcgag atcacgttgg tcggaatata
     1861 ggccgagacg tcgttggcct gcgtctcgat gatcggcagg gcggtcagcg aaccgttgcc
     1921 attgtcgtcg ttcagcttgg cggcgcgctc aagcaggcgc gaatgcaggt agaaaacgtc
     1981 gcccggatag gcttcgcggc ccggaggacg gcgaagcagc agcgacatct ggcgataggc
     2041 gacggcctgc ttcgacagat cgtcataaga gatcagcgcg tgcatgccgt tgtcgcggaa
     2101 atattcgccc atggtgcagg ccgtgaacgg cgccaggaac tgcatcggcg cgggatcgga
     2161 agcggtggcc gcaacgatga tcgaatattc gagcgcgccg cgctcttcca gcaccttgac
     2221 gaactgcgcg accgtcgagc gcttctggcc gacagcgacg tagacgcaat aaagcttctc
     2281 ggcgtccgga ccgttgtcgt ggaccgactt ctggttgagc atggcgtcga ggatgatggc
     2341 ggtcttgccg gtctggcgat cgccgatgac cagttcgcgc tggccacggc caaccgggat
     2401 cagcgcatcg atggccttga ggccggtcga catcggctcg ttgaccgact tgcgcgggat
     2461 gatgccgggc gccttgacgt cgacgcgctt gcgctcggta tatttgatcg ggcctttgcc
     2521 gtcgatcggg ttgcctagcg cgtcgacgac gcggccaagc aggcccatgc cgatgggcgc
     2581 atcgacgatg gcgccggtgc gcttgacgac gtcgccttcc ttgatgtcgc ggtcggcgcc
     2641 gaagataacc acgccgacat tgtcggcttc gaggttgagc gccatgccgc ggatgccgcc
     2701 ggggaactcg accatctcac cggcctggac attgtcgagg ccgtagacgc gggcaatgcc
     2761 gtcgccgacg gacagaacct ggccgacttc ggagacctcg gcctccttgc cgaaattctt
     2821 gatctgatct ttcagaattg cggaaatttc cgcagcgcgg atgtccatca gccgacctct
     2881 ttcagtgcaa gcttaagcga attgagtttg gttttgagcg acgtatcgat ctggcgcgaa
     2941 cccatcttga ccaccagccc gccgagaagc gacggatcga cggtgacggt gatcgccacg
     3001 tccttgccgg ccacgctttt cagcgtcgcc ttcagttcgg tttgctgggc cgccgtcagc
     3061 tcatgggccg aagtgacttc ggcggacgtt tcgccgcgat gctcggcagc gatccggcga
     3121 aacgccttga tcatgcccgg caccgcgaac aggcggcggt tggcggcgac gacgcgcagg
     3181 aaattaccgg caagcccggt gatgccggcc ttgtcggcga ccgcggcgat ggccttggcc
     3241 tggtcttcgc tggagaatac cgggcttttg atcagacgtt cgaggtcggc gctgttggcc
     3301 agcatggttt cgaagctggt caggtcggcc tcgaccttgg cgacggagtt cgcctgcgat
     3361 gcgaggtcga acagcgaacc tgcatagcgt tctgcaacac ccgagattgg cgatgacgat
     3421 tgagccacgg accgtctttt ctcttgtctg agaggccgca gattgttggc gcgagcaaaa
     3481 actctggcta aatctttggc cttacttcat tttttcaagc gcaggggtgc ggcttccgca
     3541 tccccgagcc gaaagtcgat tgccgtctag cacaggcctt ttaagaccgc aatgcgtcgc
     3601 cagaccggtt tcgaggcact attgtcgcat ccggcggttc agttcaacca ccggctcccg
     3661 aatagtgcgc gattcagggc aaaaagccga aagcgaaggc cagcggcagg gcggccagta
     3721 tcagttcgac agcggcggaa gcccagttgt aaggcgtatt ggcccggtcc gacatcatcg
     3781 acagcaggcg gccaaaggcc gtgaacagcc aggaaaagcc gagcgccata taaagcaacg
     3841 gttgcgccag caggatgcag cagaggccga cgccgagata aaaccccgac atgcgtcccc
     3901 ttgcctcggc gatcgccgcc ggcttttccg gtttggcttg cagccgcaga acccgaaacg
     3961 agatgccggg cgcaagaaac agcaacaggc caagcagcac cgttaccacc gcgctcgacc
     4021 aagccagcca ctcgccctgg ctcatcggcc acggaaaaga atattccatc agctgcccct
     4081 ctcctcaatc cgcatttttt gcatctttac ccatcccgtt ttgtgacgcc agcgcgggcg
     4141 cgcgaaattc gcgcacgggc acggctgtcg tcacaaaagc ccacctcaca ggaagctttg
     4201 cgggtcgata tcgacctgca cgcgcaccga gccgcgtact ttcgggccgg ccgccagcag
     4261 cgtgcgcacg aagccttgca tgtcggcgcg tttttcgccc tggatcaaca ggcggaagcg
     4321 gtggcgtccg cccagcagcg ccagcggcgc ttcggcgggg cccagcacga atatgtcggt
     4381 ggcttcgggc gcggcgcgac gcaggccgcg cgcgtggcct tctgcttcgg cgcgcgacgc
     4441 ggcgctgacg ataattccgg ccagacggcc gaagggcggc a
//
LOCUS       DASYCS010000440         4637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_712892,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000440 DASYCS010000000
VERSION     DASYCS010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4637
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712892"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VGM46_13425"
     CDS             <1..324
                     /locus_tag="VGM46_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEY4193641.1"
                     /translation="KPVAASTGASNRLAAPRGGRADDLLAIKGIGPVNQRKLNEHGIF
                     HFDQVAAWRKADIEAVEAYLAFDGRIAREDWIGQARTLAKAAAKQASATTKATPATAK
                     RGGRK"
     gene            324..1049
                     /locus_tag="VGM46_13430"
     CDS             324..1049
                     /locus_tag="VGM46_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4193642.1"
                     /translation="MAGKTLLDIKGVHTFYGNIQALNGVDVNVDEGEIVALIGANGAG
                     KSTLMMTIFGAPRARSGSIVFAGNDITKLPTHEIARLRIAQSPEGRRIFPRMTVMENL
                     QMGASLDNLKYFDEDVEKIFTLFPRLKERIAQRGGTLSGGEQQMLSIGRALMARPKLL
                     LLDEPSLGLAPLIVKQIFDAIRELNRTEGLTVFLVEQNAFGALKLATRGYVMVNGNVT
                     MSGTGKELLANPEVRAAYLEGGR"
     gene            1161..1508
                     /locus_tag="VGM46_13435"
     CDS             1161..1508
                     /locus_tag="VGM46_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193643.1"
                     /translation="MQGILHEEPSIWQFFLVTCVLGGWAAWMTGRACAQTWRSPTNLF
                     IYLLGLGLGVRFIHHALFNGTMFSLQYYIVDTIVLMILGFIGYQYTRTNQMVTQYHWL
                     YERASVLSWKSKG"
     gene            1648..2769
                     /locus_tag="VGM46_13440"
     CDS             1648..2769
                     /locus_tag="VGM46_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007816032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY4193644.1"
                     /translation="MKKSLLSAVALTAIVAFSGNAWADVLMGVGGPITGPNAAYGAQL
                     QKGAEQAVADINAAGGMNGEQIKLSIGDDQSDPKQGISVANKFVADGVKFVDGHFNSG
                     VTIPASEVYAENGILVVSPAATNPQLTERGLANVFRTCGRDDQQGGIAGAYLASHFKD
                     GKIAVIHDKTPYGQGLADETKKAMNAAGVTEVMYEGVNVGDKDFSALIGKMKEAGVTI
                     IYWGGLHTEAGLIIRQAADQGLKATLVSGDGIVSNELASIAGDAVAGTLNTFGPDPRL
                     IPGNKELVEKFRSQGFEPEAYTLYSYASVQAIVAAANAIKSNDPLEVAKALHEKGPFK
                     TVLGDLSFDEKGDPKLPGYVMYEWKKGADGKYTYTQKAD"
     gene            2958..>4637
                     /locus_tag="VGM46_13445"
     CDS             2958..>4637
                     /locus_tag="VGM46_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006204858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEY4193645.1"
                     /translation="MAITKILVANRSEIAIRVFRAASELGLKTVAIWAEEDKYSLHRF
                     KADESYQVGRGPHLAKDMGPIESYLSIEEVIRVARLSGADAIHPGYGLLSESPEFAEA
                     CAEAGIVFIGPKPDTMRRLGNKVAARNLAIEVGVPVVPATEPLPDDIEAVKKLAASIG
                     YPVMLKASWGGGGRGMRAIRAEADLAREVMEGKREAKAAFGKDEVYLEKLIERARHVE
                     VQVLGDTHGNAVHLFERDCSIQRRNQKVVERAPAPYLDEARRQELCGHALKIARHTAY
                     IGAGTVEFLMDADSGKFYFIEVNPRIQVEHTVTEQVTGIDIVKAQIHILDGFAIGTPQ
                     SGVPAQEDIRLNGHALQCRITTEDPEHNFIPDYGRITAYRGATGFGIRLDGGTAYSGA
                     VITRFYDPLLEKVTAWAPTPAETISRMTRALREFRIRGVATNLTFLEAIINHPSFADN
                     SYTTKFIDNTPELFAHVKRQDRATKLLNYLADVSVNGHPETRARARPNPDAAAPVIPW
                     LNSDIPDGTKQRLDALGPQKFGQWMRDQKEVLVTDTTMRDGHQSLLATRVRT"
BASE COUNT          936 a         1421 c         1438 g          842 t
ORIGIN      
        1 aagcctgtag ctgcatcgac gggcgcatcc aaccggttgg ctgcgccgcg cggcggcagg
       61 gccgacgatc tgctcgccat caagggcatc gggccggtca accagcgcaa gctcaacgag
      121 cacggcatct tccatttcga ccaggtcgcg gcgtggagga aggcggatat cgaggcggtg
      181 gaagcctatc tggctttcga cgggcgcatt gcgcgcgaag actggatcgg ccaggccaga
      241 acattggcca aggcggcggc caagcaggct tccgccacca cgaaggcgac gccggcgaca
      301 gccaagcgcg gagggcgtaa ataatggccg gcaaaacgct gctcgacatc aagggcgttc
      361 atactttcta cggcaacatc caggctctca atggcgtcga cgtcaatgtc gatgaaggcg
      421 agatcgtggc cctgatcggc gcgaacggtg ccggcaagtc cacgttgatg atgacgatct
      481 tcggcgcgcc acgcgcgcgt tcgggcagca tcgtttttgc cggcaacgat atcaccaagc
      541 tgccgaccca cgagatcgcg cggctgcgca tcgcgcagtc cccggaagga cggcggattt
      601 tcccgcgcat gacggtgatg gagaacctcc agatgggggc gagcctcgac aacctcaaat
      661 atttcgatga ggacgtcgag aaaatcttca cgctgtttcc gcgtctcaag gagcggatcg
      721 cccagcgcgg cggcacgctt tccggcggcg agcagcagat gctgtcgatc ggccgcgcgc
      781 tgatggcgcg tccgaagctg cttttgctgg acgagccgtc gcttggcctg gcgccgctga
      841 tcgtcaagca gatattcgac gccattcgcg agctgaaccg cacggaaggg ctgaccgttt
      901 tccttgtcga gcaaaacgcc tttggcgcac tcaagctcgc cacgcgcggc tatgtcatgg
      961 tcaatggcaa tgtgacgatg agcggcaccg gcaaggagct tctcgccaat ccggaagttc
     1021 gcgccgccta tctcgaaggc ggccgttagg gcgtgtctcc cgagccggaa agccggcttc
     1081 gggaaggcat gtgtgagaac aagagcctga agcgatgtcg gcggtttcga tgagctttgg
     1141 caaatttggg agcgatcgac atgcagggca ttcttcatga agaaccctcg atctggcagt
     1201 tctttctcgt aacctgcgtg cttggcgggt gggctgcatg gatgacgggc agggcctgcg
     1261 cccagacatg gcgctcgccg acgaacctgt tcatctacct gctcggcctc ggactgggtg
     1321 tccggttcat ccatcatgcg ctgttcaacg gcacgatgtt ttctttgcaa tactacatcg
     1381 tcgatacgat tgttctgatg atcctgggtt tcatcggcta tcaatacacg cgcaccaacc
     1441 aaatggtgac gcagtatcat tggctttacg agcgcgcctc ggtcctgagc tggaagtcaa
     1501 aaggctgacg ttcatgatta acgccgattc ggggatgaat cggcgtgaca aggcctctaa
     1561 aatcggcaaa gtatactttc tgcgataatg ggtgggcatc gcatgaaagc atcatccacg
     1621 cccactccgt aaatgggagc gttatacatg aagaaatcac tcttgtccgc cgtcgcgctg
     1681 accgcgatcg tcgcgttcag cgggaatgcg tgggctgatg tcctgatggg cgtcggcggc
     1741 ccgatcaccg gtccgaacgc agcctatggc gcgcaattgc agaagggcgc cgagcaggct
     1801 gtcgccgaca tcaatgccgc gggcggcatg aatggcgagc agatcaagct cagcatcggc
     1861 gacgaccagt cggaccccaa gcagggcatt tccgttgcaa acaagttcgt tgccgacggc
     1921 gtcaagttcg tcgacggcca cttcaattcg ggcgtcacca ttcctgcctc ggaagtctat
     1981 gccgaaaacg gcatcttggt ggtttcgccg gccgcgacga atccgcagct gacggagcgt
     2041 ggcctggcga acgtgttccg cacctgcggt cgtgacgacc agcagggcgg catcgccggc
     2101 gcctatctcg cctcgcactt caaggacggc aagattgccg tcatccatga caagacgcca
     2161 tacggccagg gcctcgccga cgaaaccaag aaggcgatga acgccgcggg cgtcaccgaa
     2221 gtcatgtatg aaggcgtcaa tgttggcgac aaggacttct ccgcgctgat tggcaagatg
     2281 aaggaagccg gcgtcaccat catctattgg ggcggcctgc acacggaagc cgggctgatc
     2341 atccgtcagg ccgccgacca gggcctgaag gccacgctgg tctccggcga cggcatcgtg
     2401 tcgaacgagc tggcctcgat cgcgggtgac gcggttgccg gcacgctcaa cactttcggc
     2461 cccgatccgc gcctgatccc gggcaacaag gaactggttg aaaagttccg ttcgcagggc
     2521 ttcgagccgg aggcctatac gctctactcc tatgcttcgg tgcaggccat cgttgcggcg
     2581 gcaaacgcga tcaagtccaa tgatccgctg gaagtcgcca aggccctgca tgagaagggc
     2641 ccgttcaaaa cggtgctcgg cgacctgtcc ttcgacgaga agggcgatcc gaagcttccg
     2701 ggctacgtca tgtatgagtg gaagaagggt gccgacggca agtacaccta cacccagaag
     2761 gccgattgat attggcctga cctatcgtcg ccaacccttt ggcggcaacg atgcccggcg
     2821 ctctgcgccg ggcatttttg ttttgccggc ggataattga aacaggccga tccgtcggcg
     2881 cagcattttt gtttgcgctg caacattttc acgttattca ttcgcgctgc ctaatccaat
     2941 ccatgtggag cgcgtccttg gccatcacga aaatcctcgt cgccaatcga tccgaaatcg
     3001 ccattcgcgt gtttcgcgca gccagcgagc tgggcctgaa aaccgtggcg atctgggccg
     3061 aagaggacaa atattcgctg catcgcttca aggccgacga aagctaccag gtcgggcgcg
     3121 gcccgcatct ggccaaggac atggggccga tcgaaagcta cctgtcgatc gaggaggtga
     3181 tccgcgtcgc caggctttcc ggcgccgatg ccatccatcc cggctatggg cttctgtccg
     3241 aaagcccgga attcgccgag gcgtgcgccg aggccggcat cgtcttcatc gggccaaagc
     3301 cggatacgat gcgccggctg ggcaacaagg tcgccgcgcg caaccttgcc atcgaggtcg
     3361 gcgtgccggt ggtgccggcg accgagccgc tgcccgacga cattgaagct gtgaagaagc
     3421 ttgccgcatc gatcggctat ccggtcatgc tcaaggcttc gtggggcggc ggcgggcgcg
     3481 gcatgcgcgc catccgcgcc gaagccgatc tcgcgcgcga agtgatggaa ggcaagcgcg
     3541 aagccaaggc ggccttcggc aaggacgagg tctatctgga aaaactgatc gagcgcgccc
     3601 gccatgtcga ggtgcaggtg ctgggggata cgcacggcaa cgccgtccac ctgttcgagc
     3661 gcgattgttc gatccagcga cgcaaccaga aggtggtcga gcgggcgccc gcaccttacc
     3721 tcgatgaggc ccggcggcag gaactgtgcg gccatgcgct gaagatcgcg cgccacaccg
     3781 cttatatcgg ggccggcacg gtcgagttcc tgatggatgc cgatagcgga aaattctatt
     3841 tcatcgaagt caatccgcgc atccaggtcg agcataccgt caccgaacag gtgaccggca
     3901 tcgatatcgt caaggcgcaa atccacatcc tcgacgggtt tgccatcggc acgccgcaat
     3961 cgggcgtgcc ggcgcaagag gatatccggc tcaacggcca cgccctgcaa tgccgtatca
     4021 ccacggaaga tcccgagcac aatttcattc ccgattatgg ccgcatcacc gcctatcggg
     4081 gtgctaccgg cttcggcatc aggctggatg gcggcaccgc ctattcgggc gcggtcatca
     4141 cccggttcta cgatccgctg ctggaaaagg tgacggcgtg ggcgccgaca ccggccgaga
     4201 cgatctcgcg catgacgcgg gcgctgcgcg agttccgcat tcgcggcgtc gccaccaacc
     4261 tcaccttcct cgaagcgatc atcaatcacc cgagctttgc cgataattcc tatacgacta
     4321 agttcatcga caatacgcct gagctgtttg cgcatgtgaa gcggcaggac cgcgccacca
     4381 agctgctcaa ctacctggcc gatgtctcgg tcaacggcca cccggaaacg cgggcgcggg
     4441 cgcggccgaa cccggatgcg gcggcacccg tcatcccctg gttgaacagc gatattccag
     4501 acggcaccaa gcagaggctc gacgcgctcg gcccgcagaa gttcggccag tggatgcgcg
     4561 atcaaaaaga ggtgctcgtc accgatacga ccatgcgcga cggccaccag tcgctgctcg
     4621 ccacccgcgt tcgcacg
//
LOCUS       DASYCS010000441        11374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4189324,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000441 DASYCS010000000
VERSION     DASYCS010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 11374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11374
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4189324"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VGM46_13450"
     CDS             <1..381
                     /locus_tag="VGM46_13450"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEY4193646.1"
                     /translation="KNFLYLVQKDGKARRYGVGTGKPGFEWAGTHKITQKREWPDWRP
                     PAEMIAREAARGRSLPAHMEGGIENPLGARAMYLGSTLYRIHGTNQPWTIGGSVSSGC
                     IRMRNQDVVDLYERVPVGTSVVVM"
     gene            631..1545
                     /locus_tag="VGM46_13455"
     CDS             631..1545
                     /locus_tag="VGM46_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013530353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HEY4193647.1"
                     /translation="MSDDTRDLKGGPVARRIMAEVTRDAAKATAEGFPPKLVSITVGD
                     VAAVSVYVRNQRAKAELAGIGFEERNFPADLTASELEAAIHGMNADPRVTGIIIQRPV
                     PAHIPIKAIQAAVHPLKDVEGMHPASIGNIVYNELDLAPCTAAASVELLKETGLDLKG
                     LEVVVVGHSEIVGKPIAFLLMSEGATVTVCHHMTRSVAAHARRADALFVAVGKPRLIQ
                     ADMVKPGAAVIDIGINSEIGPDGKSRVVGDVDTESVRQVASWITPVPGGVGPITVSIL
                     LRNTMVALARQRALYQATYGAPDRTAAE"
     gene            complement(1831..3147)
                     /gene="glcF"
                     /locus_tag="VGM46_13460"
     CDS             complement(1831..3147)
                     /gene="glcF"
                     /locus_tag="VGM46_13460"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018426619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HEY4193648.1"
                     /translation="MQTNFSLAQLADPHVAESEKILRKCVHCGFCTATCPTYVTLGNE
                     LDSPRGRIYLIKDMLENGRPADEQIVTHIDRCLSCLACMTTCPSGVNYMHLVDHARAH
                     IEKTYKRPLPNRAIRALLAFVLPHSGRFRAALKLARLGKPFIGAFDRIPALKPLGAML
                     RLAPASVPAPSLMAKPGVHPAAGGARERKRVAILTGCAQPVLDPGINEATISLLNRLG
                     VEVVVPQGEGCCGALVHHMGREEQALASARNNVDAWTRQIESGGLDAIIVTTSGCGTT
                     IKDYGFMLRLDPVYAEKAARVSALARDITEYLATLDLPEPVQKPGTVVAYHSACSMQH
                     GQKIVRQPKELLARAGFVVREPREGHLCCGSAGTYNIMQPEISARLRDRKVRNIAATG
                     AAIVATGNIGCITQIASAANMPVVHTVKLLDWAYGGPRPEGVVGGE"
     gene            complement(3151..4356)
                     /locus_tag="VGM46_13465"
     CDS             complement(3151..4356)
                     /locus_tag="VGM46_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013530351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY4193649.1"
                     /translation="MTTFTPTSADEVLSTVQWALSEDKPLEIVGHGSKRGIGRPSQAQ
                     HTLDLSKLSGVTLYEPAELVLSARAGTPLTEIEKLLADNGQQFAFEPMDYSALLGGER
                     GCGTIGGMLAVNLSGPRRLKAGAARDHVLGIHAVSGRGEAFKSGGRVVKNVTGYDMSK
                     LMANSWGTLAVCTDVTFKVLPAAETETTLAVRGLLDDRAAAAMALALGSSAEVSSAAH
                     LPYPAVARVADGALGNDAATLLRVEGFGPSVAYRIETLKSLLKNAGPLEEISGETSGK
                     IWSDIRDCAPFADGGEKPVWRVSMAPADGHAMVMQLRMRAAVSAFYDWQGGLIWLRME
                     HGEPEAALLRALIKHHGGGHATLVRATPAERAALPVFEPQEAALASLSARLKEQFDPK
                     NILNPGRMA"
     gene            complement(4359..5855)
                     /locus_tag="VGM46_13470"
     CDS             complement(4359..5855)
                     /locus_tag="VGM46_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008509458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY4193650.1"
                     /translation="MSGLAMPKPDEATLSRRAEIVADMRQIVPGEGVVDAANAMRAFE
                     SDGLTAYRQLPLVVVLPETVAQVSRILKYCDQRKIRVVPRGSGTSLSGGALPLEDAVL
                     LVMSRFNRILDIDFPNRVVVAQPGVTNLGITTAVEQEGFYYAPDPSSQIACSIGGNVA
                     ENSGGVHCLKYGLTANNLLGIEMVLMNGEVVRLGGKHLDAEGYDLLGVMTGSEGLLGV
                     VTEVTVRILKRPETARALLIGFPTSEQGGQCVADIIGAGIIPGGMEMMDRPAIHAAED
                     FVHADYPLDVEALLIVELDGPGVEVDHLIMAVEAIAIRNGSTTCRISQSEQERLTFWA
                     GRKAAFPAVGRISPDYYCMDGTIPRKELPKVLHGMKELSKKYGLGVANVFHAGDGNLH
                     PLILYDANVPGELEKAEGFGSDILRLCVKVGGVLTGEHGVGVEKRDLMPEMFNAIDLD
                     QQMRVKCAFDPNHLLNPGKVFPQLRRCAELGRMHISGGKLPFPEIPRF"
     gene            complement(6003..7325)
                     /locus_tag="VGM46_13475"
     CDS             complement(6003..7325)
                     /locus_tag="VGM46_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010914419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3422 family protein"
                     /protein_id="HEY4193651.1"
                     /translation="MSNEPANLEFQPRATGSVMGFPCHEGRSGALGEVHARPHPLVPT
                     PRVIMQLSFMTEGGASVDHAVLCELSRRLGVAAPERQARHYAMKWGNGSLRWERHTEF
                     STYLFEGPLAGNGRAQEESPFGNGFSPPGTVISGIRLEIRKWGAASEKLIAGFDPTSL
                     CYSLVENGKAAIITDFRQDGDGLTRILVLDRGLTPARTGALSQRLIDIETYRTLAMLG
                     LPLALTLSGRARRIEDRLAVITREMKVAETRDSQTLLADLTELAAELEADAASSLYRF
                     GASRAYDGIVGERLEALDEEPVPGYDTWAAFLQRRVAPAMRTCRSVEERQANLSRKLT
                     RATTLLRTWVDVEVEKQNRDLLASMNNRARLQLRLQQTVEGLSVAAVSYYVVGLIGYL
                     AKGASFFGHAFAPEVVTAASVPVAVLLVWWGVRHIRRSHMEPGKAPGE"
     gene            7532..8320
                     /locus_tag="VGM46_13480"
     CDS             7532..8320
                     /locus_tag="VGM46_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEY4193652.1"
                     /translation="MNDIFSRIEHSRTADEVVGQIETLILEGVLSTGDRLPGERELSR
                     QFDVSRPILRDALKALELRGLLVTRHGGGTHVADIIGQVFSKPVVDLFATHRKATNDY
                     LEYRREIEGVAAEYAARRATADDLALLDAIMARMDKAHSTGDFEDEAEIDVEFHHAVG
                     ECAHNIILLHTLRSCYRLLSEGVFQNRALAFALPGAREALLEQHRAIHAAVKAGNPAA
                     ARQAAMDHITYVERAMAQAERSGDWERVSRLRLRQRAPANAVKS"
     gene            8355..9491
                     /locus_tag="VGM46_13485"
     CDS             8355..9491
                     /locus_tag="VGM46_13485"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEY4193653.1"
                     /translation="MSLDLTIADLKEKARRRVPRMFFQYADSGALTESTYAANEADFA
                     KIKLRQRVLVDMTNRTLASTMVGEKVSMPVALAPTGSTGMQHADGEMLAAQAAEEFGV
                     PFTLSTMSICSIEDVASVTTKPFWFQLYVMRDREFVNNLIDRAKAAKCSALVLTLDLQ
                     ILAQRHKDIRNGLSAPPKFTPSSIWQMASRPGWCLKMAGTKRRSFGNIVGHAKNVSNL
                     ASLSAWSHEQFDPRLSWKDVQWIKDRWGGKLILKGVLDKEDAIMAAQSGADAIIVSNH
                     GGRQLDGAPSSISVLEQIADAVGDKIEVHMDGGIRSGQDVLRALCLGAKGVHIGRPFL
                     YGLGAGGKAGVTRALEIIRNELDTTLALCGKRDILDVGKHMLHR"
     gene            9520..10314
                     /locus_tag="VGM46_13490"
     CDS             9520..10314
                     /locus_tag="VGM46_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY4193654.1"
                     /translation="MSGIHLRDARAPEGMRLHAIGDVHGRLDLLYAMHRRIAEDMARD
                     PPADWRIVHLGDYVDRGPASKGVLDFLIAVRAIEPRNIMLGGNHDVAFLDFLERPDAS
                     GVFALYGGVQTAKTYGVDLAIGNDAPAGQAEEALRSGHAQLLRAVPQSHVDFLRGLIF
                     CASFGDFFFCHAGIRPGVALDRQDREDLIWIREAFHDYPGLYPKVIVHGHTPHRLPEI
                     KANRVNVDTLAHKSGRLTALVVDGAKKEILTVVEDGSVQRSEAVTP"
     gene            complement(10287..10427)
                     /locus_tag="VGM46_13495"
     CDS             complement(10287..10427)
                     /locus_tag="VGM46_13495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193655.1"
                     /translation="MTTVKAAFLSFAMVSAIVLCGLGIYAADAASNHNAVDGYGVTAS
                     LR"
     gene            10622..10828
                     /locus_tag="VGM46_13500"
     CDS             10622..10828
                     /locus_tag="VGM46_13500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193656.1"
                     /translation="MSIRGGFGAACQNCNDRRVMRSEFRIGATYSQTMAVGNIRDEAV
                     STVSKNSGIVEIRDDRHTHRNLAA"
     gene            10919..>11374
                     /locus_tag="VGM46_13505"
     CDS             10919..>11374
                     /locus_tag="VGM46_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008877383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193657.1"
                     /translation="MPLQNRVDPFGVIHAVAARGLFTGNRGIIHDPATKKLLNKRWTL
                     KAWIICTCEFKGRRREPMGRNRPGKNGEPAGAGWTELFFLDEVTALAAGHRPCFYCQR
                     ERAVDFVRRFGEAFGLDEPRAPMLDSRLHRERLASGGRLPTIEASELAGL"
BASE COUNT         2052 a         3625 c         3734 g         1963 t
ORIGIN      
        1 aagaatttcc tctatctcgt ccagaaggac ggcaaggcgc ggcgctacgg cgtcggcacc
       61 ggcaagcccg gcttcgaatg ggccggcacc cacaagatca cgcagaagcg cgaatggcca
      121 gattggcggc cgccagccga aatgatcgcg cgcgaagcgg ccagagggcg ctccctgccg
      181 gcgcatatgg aaggcggcat cgaaaacccg ctcggcgcgc gggccatgta tctcggatcg
      241 acgctttacc gcatccacgg caccaaccag ccatggacca tcggcggctc ggtgtcgtcg
      301 ggctgcatcc ggatgcgcaa ccaggacgtg gtcgatctct acgagcgggt gccggttggc
      361 accagcgtcg tggtgatgta gcctttcaag gcaataacgg gggacgggcc ttgtggggac
      421 atggccggtc gaaaaaaggg cagcgccgct tcggttgcgc tgcccttttt cgttgtccgg
      481 ggttggctgg cggtccttgc cgcgtactcg cctgaacatt tcgaaagccg cctttcggtc
      541 gcatcgagag taacgcggtt cgctttcacg agggcgacgg ctgcgcgctc ctgctacgcc
      601 atcgctgaaa ccagaggcga aggctttgcc atgtccgacg atacgcgcga cctcaaaggc
      661 ggccctgtcg ccaggcgcat catggccgaa gtcacccgcg acgcggcaaa agccacggcg
      721 gaaggctttc cgcccaagct ggtgtcgatc accgtcggcg atgtggcagc ggtcagcgtc
      781 tatgttcgaa accagcgcgc caaggccgaa cttgccggca tcggcttcga agagcgcaat
      841 tttccggctg acctgacggc atcggagctg gaagcggcga tccacggcat gaatgccgat
      901 ccgcgcgtca ctggcatcat catccagcgc ccggtgccgg cgcatatccc gatcaaggcg
      961 atccaggcgg cggtgcatcc gctgaaggac gtggagggca tgcacccggc ctccatcggc
     1021 aacatcgtct ataacgaact cgacctcgcg ccctgcacgg cggcggcctc ggtggaactg
     1081 ctcaaggaaa ctggcctcga cctcaagggg cttgaggtgg tggtggtcgg ccattcggag
     1141 attgtcggca agccgatcgc cttcctgctg atgagcgaag gcgcgacggt gacggtctgc
     1201 caccatatga cgcgttcggt ggctgcgcat gccaggcggg cggacgcgct gttcgtcgcg
     1261 gtgggcaagc cgcgcctcat ccaggcggac atggtgaagc cgggcgccgc cgtcatcgat
     1321 atcggcatca attccgagat cggcccggac ggcaagagcc gcgtcgtcgg cgatgtcgat
     1381 accgaaagcg tgcgccaggt ggcgtcgtgg atcacaccgg tgccgggcgg cgtcggcccg
     1441 atcaccgtgt cgatcctgct gcgcaacacc atggtggcgc tggcgcgcca gcgcgcgctg
     1501 taccaggcaa cctacggcgc gccggaccgg accgcggcgg aataaggttg gcgcggtagc
     1561 agcgcgaggt gggtcggcgg cgaagaaccc ctctccgtct cgctgcgcga gcacctctcc
     1621 cccaaagggg gcgaggaacc agagttcggc ttgatccggg ccgagcagac gcggcgttcg
     1681 ccctcacaat agtcgttcga atgatcgaac ctgttgccgc taactttgct gaagtggctg
     1741 acttatgcag attccggttc ctcgccccca tcggggaaga ggtggttcga gcagcgagac
     1801 ggagagggga ccttcacacc cgaagacgtg ctactccccg ccaaccaccc cttccggcct
     1861 cgggccgcca taggcccagt cgagcagctt taccgtgtgc accaccggca tgttggcggc
     1921 ggaggcgatc tgggtgatgc agccgatgtt gccggtggcg acgatggccg cgccggttgc
     1981 cgcgatgttg cgcaccttgc ggtcgcgcag gcgcgccgag atttccggct gcatgatgtt
     2041 gtaggtgccg gccgagccgc agcacaggtg cccctcgcgc ggctcgcgca cgacaaagcc
     2101 tgccctggcc agcaattcct tcggctggcg aacgatcttc tggccgtgct gcatcgagca
     2161 ggccgaatga taggcgacga cggtgccggg cttttgcacc ggttccggca aatcgagcgt
     2221 ggcgagatat tcggtaatgt ccctggccag cgccgacacc ctggccgcct tttcggcata
     2281 gactgggtcg agccgcagca tgaagccata atccttgatg gtcgtgccgc agcctgatgt
     2341 ggtgacgatg atggcgtcga ggccgccgct ctcgatctgg cgcgtccagg catcgacatt
     2401 gttgcgcgcc gaggccagcg cctgctcctc gcggcccatg tgatggacca gcgcgccgca
     2461 gcagccctcg ccttgcggca ccacgacttc gacgcccagc cggttgagca gcgagatcgt
     2521 cgcctcgtta atgcccggat cgagcaccgg ttgcgcgcaa ccggtgagga tggcgacacg
     2581 cttcctttcg cgcgccccgc ccgccgccgg atggacgccg ggcttggcca tcagggatgg
     2641 cgccggcacg gaagcgggcg ccaacctcag catggcgcca agcggtttca gcgccggtat
     2701 acggtcgaac gcgccgatga acggcttgcc cagcctcgcc agtttcaacg ccgcgcgaaa
     2761 ccgccccgaa tgcggcagca cgaaagccag caaggcccga atggcccggt tgggcagcgg
     2821 ccgcttgtag gtcttctcga tatgggcgcg ggcgtgatcg accagatgca tgtagttgac
     2881 gcccgaggga caggtcgtca tgcaggcgag gcaggacagg cagcggtcga tatgggtgac
     2941 gatctgctcg tcggcggggc ggccattctc cagcatgtcc ttgatgagat agatgcgccc
     3001 gcgcgggcta tccagctcgt ttcccagcgt cacataggtc gggcaggtag cggtgcagaa
     3061 gccgcaatgc acgcatttgc gcaggatctt ttccgattct gcaacgtgcg gatcggccag
     3121 ttgcgcgagg gagaaattgg tctgcatcgc ctacgccatc cggcctgggt tgaggatatt
     3181 cttcgggtcg aactgttctt tcagccgtgc ggacaggctg gcaagcgcgg cctcctgcgg
     3241 ctcgaacacg gggagcgcgg cgcgctcggc gggcgttgcc cgcaccagcg tcgcgtggcc
     3301 gccgccatgg tgttttatca gcgcgcgcaa cagggcggct tccggctcgc catgctccat
     3361 gcggagccag atcagcccgc cctgccagtc gtagaaggcc gagacggcgg ctcgcatgcg
     3421 aagctgcatc accatggcgt ggccatccgc cggcgccatc gacacccgcc acaccggctt
     3481 ttcaccgcca tcggcgaacg gcgcgcaatc gcggatgtcg gaccatatct tgcccgacgt
     3541 ttcgcctgaa atctcttcca gcgggccggc gttcttgagc aggcttttga gcgtctcgat
     3601 gcggtaggcg acggagggac caaacccttc gacgcgcagc aaggtggccg cgtcattgcc
     3661 gagcgcgcca tcggcaacgc gcgcgacggc gggatagggc agatgcgcgg cgctcgaaac
     3721 ctcggcgctg gagccaagcg ccagcgccat ggcggcggcg gcccgatcgt ccagcaagcc
     3781 gcgcacggcg agcgtcgttt cggtctcggc ggcgggcaga accttgaagg tgacgtcggt
     3841 gcaaacggcc agcgtgcccc agctattggc catcagcttc gacatgtcgt agccggtgac
     3901 gttcttgacg acgcgcccgc ccgacttgaa cgcctcgccg cgccccgaca ccgcatggat
     3961 gcccagcaca tggtcgcgcg ccgcgcctgc tttcagccgg cgcgggccgg aaagattgac
     4021 ggcgagcatg ccgcctatcg tgccgcagcc ccgttcaccg ccgagcaggg ccgaataatc
     4081 catcggctcg aaagcgaatt gctggccgtt atcggccagc aatttctcga tttccgtcag
     4141 cggcgttccg gcgcgcgccg acagcaccag ttccgcaggt tcgtaaagtg tgacgcccga
     4201 aagcttcgac agatcgagcg tatgctgcgc ctgcgagggc cggccaatgc cgcgcttcga
     4261 gccatgaccg acgatttcca gcggcttatc ctccgacaac gcccactgga cggtggacag
     4321 gacttcatca gcggaggtgg gggtgaaggt ggtcaagctc aaaacctcgg aatttccggg
     4381 aagggcagtt tgccgccgga gatgtgcatg cggccgagtt cggcgcagcg gcgcaattgc
     4441 ggaaagacct tgcccggatt gagcagatgg ttgggatcga aggcgcattt gacgcgcatc
     4501 tgctggtcga ggtcgatggc gttgaacatt tccggcatca ggtcgcgctt ttcgacgccg
     4561 acgccatgct cgccggtcaa tacgccgccg accttgacgc atagccgcaa aatatcggag
     4621 ccgaagcctt ccgccttttc cagttcgccc ggcacattgg catcatagag gatgagcgga
     4681 tgcagattgc cgtcgccggc atggaagaca ttggcgacgc caaggccgta tttcttcgac
     4741 agctccttca tgccgtgcag gactttcggc agctccttcc tcggaatggt gccgtccatg
     4801 cagtaataat ccggcgagat gcggccgacc gccgggaacg ccgccttgcg accggcccag
     4861 aaggtcagcc gctcctgctc gctctgcgaa atgcggcagg tggtcgagcc gttgcggatg
     4921 gctatggctt cgaccgccat gatcaggtga tcgacctcga cgcccggccc gtccagttcg
     4981 acgatcagca acgcctcgac gtcgagcgga tagtcggcat ggacgaagtc ctccgccgca
     5041 tgaatggccg ggcggtccat catctccatg ccgcctggaa tgatgcccgc gccgatgatg
     5101 tcggccacgc actggccgcc ctgctcgctg gtcggaaagc cgatcagcaa ggcgcgcgcc
     5161 gtctccggtc tcttgagaat gcgcaccgtg acctcggtga cgacgccaag caggccttcc
     5221 gagccggtca tgacaccgag cagatcatag ccttccgcgt ccagatgctt gccgcccagg
     5281 cgcaccactt cgccgttcat cagcaccatc tcgatgccga gcaaattgtt ggcggtcagc
     5341 ccgtatttca ggcaatgcac gccgccagaa ttttccgcga cattgccgcc gatcgagcag
     5401 gcgatctggg aggatggatc gggggcgtag tagaaaccct cctgctcgac ggcggtggtg
     5461 atgccgagat tggtgacgcc cggctgggcg acgactacgc ggttcgggaa atcgatgtcg
     5521 agaatgcggt tgaagcggct cattaccaga agcacggcgt cttccagcgg cagcgcgccg
     5581 cccgaaagcg aggtgccgga gccgcgcggc acgacgcgga tcttgcgttg gtcgcaatat
     5641 ttcaggatgc gcgacacctg cgccaccgtt tcgggcagca cgacgaccag cggcagctgg
     5701 cgataggccg tcagcccgtc gctttcgaag gcgcgcatgg cgttggcggc atcgacgacg
     5761 ccctcgcccg gcacgatctg gcgcatgtcc gcgacgatct cggcgcgccg cgacagcgtg
     5821 gcttcgtcag gcttcggcat ggccagaccg gacattgcgg catcctttgc ttcactggct
     5881 tcgctttact ggtaaaaagc ttttaccagt ggccgagcct gcttgcaaat gctgcatcgg
     5941 ctaatttgtg aggcgtcatc gcctggcgtt tccgcagcgt caattggccg ctgcctttgc
     6001 gcctattcgc ccggcgcctt gcccggctcc atatggctgc ggcggatatg gcgcacgccc
     6061 caccagacca gcaggacggc aaccgggacc gacgcggcgg tgaccacttc cggcgcaaag
     6121 gcatggccga aaaaggacgc gcccttggcc agatagccga tcagcccgac cacataatag
     6181 gagacggcgg cgaccgagag gccttccacg gtctgttgca ggcgcagttg caggcgcgcg
     6241 cggttgttca tcgacgccag caggtcgcgg ttctgcttct cgacctcgac atcgacccag
     6301 gtgcgcagca gcgtggtggc acgggtgagc ttgcgcgaca ggttggcctg gcgctcctcc
     6361 accgagcggc aggtgcgcat ggcgggcgcg acgcggcgct ggaggaaggc ggcccaggtg
     6421 tcgtagcccg gcaccggctc ttcatcgagc gcctcaagcc gttcgccgac gatgccgtca
     6481 taggcgcggc tggccccgaa acggtagagg ctcgatgcgg cgtcggcttc cagttcggcg
     6541 gccagttcgg tcagatcggc cagcaaggtc tggctgtcgc gggtttcggc gaccttcatt
     6601 tcgcgggtga tgacggcaag ccggtcctcg atgcggcgcg ccctgcccga aagcgtcagc
     6661 gccagcggca ggccaagcat ggccagcgta cggtaggtct cgatgtcgat cagccgctgt
     6721 gacagcgcac cggtacgcgc cggcgtcaac ccgcggtcca gcacgagaat gcgggtcagg
     6781 ccgtcgccgt cctggcggaa atcggtgatg atggccgcct tgccgttctc gaccagcgaa
     6841 tagcagaggc tggtcggatc gaagccggct atcagtttct cggatgcggc gccccatttg
     6901 cggatttcca gccggatgcc ggaaatgacg gttcccggtg gcgaaaagcc attgccgaag
     6961 ggggattcct cctgggcgcg gccgttgccg gccagcggtc cctcgaacag gtaggtggaa
     7021 aattcggtgt ggcgctccca gcgcagcgag ccgtttcccc atttcatcgc atagtggcgc
     7081 gcctggcgtt cgggggcggc gacgcccagc cggcgcgaca gttcgcacag caccgcatgg
     7141 tcgacgctgg cgccgccctc ggtcatgaac gacaattgca tgatgacgcg cggcgtcggc
     7201 accagcggat gcgggcgggc atggacctcg cccaacgcgc ccgaacggcc ctcatggcag
     7261 ggaaaaccca tgacgctgcc ggtggcgcgc ggctggaatt cgagattggc aggctcgttc
     7321 gacacgctgg ttcccctcgg ccttgccggc cttctactcc ttgcgagtaa agaccaatca
     7381 tccgaggacg caaacggaag tttaggccgg gcgcaccgca agcgcagagt atcagctttt
     7441 aggatgagcc gcccaattgg atatataatt tgaccagttg ggcttcctgc ctttatcttt
     7501 gccggcgacg gccaacatcg tgggcaaccg gttgaacgac attttctcca ggatcgaaca
     7561 ttcgcgcacc gccgacgagg tggtggggca gatcgagacg cttattctgg aaggcgtgtt
     7621 gtcgaccggc gaccggcttc ccggcgagcg cgagctttcg cggcagttcg acgtatcgcg
     7681 gccgatcctg cgcgacgcgc tgaaggcgct ggaactgcgc ggcctgctcg tcacgcgcca
     7741 tggcggcggc acccatgtcg ccgacattat cggacaggtg ttttccaagc cggtggtgga
     7801 tttgtttgcc acgcaccgca aggcaaccaa cgactatctg gaatatcgcc gcgagatcga
     7861 gggcgtggcg gcggaatatg cggcaaggcg cgccaccgcc gacgacctcg cccttctgga
     7921 cgccatcatg gcgcggatgg acaaggcgca cagcaccggc gatttcgagg acgaggcgga
     7981 gatcgacgtc gagtttcacc atgcggtggg cgaatgcgcc cacaacatca tcctgttgca
     8041 tacgctgcgt tcctgctacc ggctgttgtc ggaaggcgtg ttccagaacc gcgcgctggc
     8101 cttcgcgctg cccggcgcgc gcgaggcgct actggagcag caccgcgcca tccacgcggc
     8161 ggtgaaggcc ggcaacccgg ccgccgcgcg ccaggcggcg atggaccaca tcacctatgt
     8221 cgagcgcgct atggcgcagg ccgagcgcag cggcgactgg gagcgcgttt cacgcctgag
     8281 gctgcgccag cgcgcgcccg ccaacgccgt caaatcctga aaaagatcaa cccgagaacc
     8341 gaccggaaca agccatgagc cttgacctga ccatcgccga tctgaaggaa aaggcgcgcc
     8401 ggcgcgtgcc gcgaatgttt tttcaatatg ccgattccgg cgcgctgacc gaaagcacct
     8461 atgccgccaa cgaggcggac ttcgccaaga tcaaactgcg ccagcgggtg ctggtcgaca
     8521 tgaccaaccg cacgctcgcc tccaccatgg tcggcgagaa agtgtcgatg ccggtggcgc
     8581 tggcgccgac gggttcgacc ggcatgcagc atgccgatgg cgagatgctg gcggcacagg
     8641 cggcggagga attcggcgtg ccgttcacgc tttccaccat gagcatctgc tcgatcgagg
     8701 atgtcgcatc ggttacgaca aagccgttct ggttccagct ctacgtcatg cgcgacaggg
     8761 agttcgtcaa caatctgatc gaccgcgcca aggccgcgaa atgctccgcg ctggtgttga
     8821 cgctcgacct gcaaatcctg gcccagcgcc acaaggatat tcgcaacggc ctttccgcgc
     8881 cgccgaaatt cacgccaagc agcatctggc agatggcgtc ccggcccggc tggtgcctta
     8941 aaatggccgg cacgaaaaga cgcagcttcg gcaatatcgt cggccacgcc aagaacgtat
     9001 ccaacctggc gtcgctttcg gcatggtcgc atgaacagtt cgatccgcgt ctgtcctgga
     9061 aggacgtgca gtggatcaag gaccgctggg gcggcaagct gatcctcaag ggcgttctcg
     9121 acaaggagga cgcgatcatg gccgcgcagt ccggcgccga cgcgatcatc gtttccaatc
     9181 atggcgggcg gcagctcgac ggcgcgccat cctcgatttc agtgctggaa cagatcgccg
     9241 atgcggtcgg cgacaagatc gaggtgcata tggatggggg catccgctcc gggcaggacg
     9301 tgctgcgcgc tttgtgcctc ggtgccaagg gcgttcatat cggccggccg tttctctatg
     9361 ggctgggtgc cggcggcaag gccggcgtga ccagggcgct ggagatcatc cgcaacgaac
     9421 tggacaccac gctggcgctc tgcggcaagc gcgacattct ggatgtcggc aagcacatgc
     9481 ttcaccgcta agttaggctc ctagcggaga ttcgcggcca tgagcggcat ccatctacga
     9541 gacgcgcgcg cgccggaagg catgcgcctc catgccattg gcgacgtgca tggccggctc
     9601 gacctgcttt acgccatgca caggcgcatc gccgaggata tggcgcgcga cccgcccgcc
     9661 gactggcgca tcgtccacct cggcgattat gtcgaccgcg ggccggcttc gaaaggcgtg
     9721 ctcgatttcc tgatcgcggt gcgcgcgatc gagccgcgca atatcatgct gggcggcaat
     9781 cacgacgtcg cctttctcga tttcctggaa cggccggatg ccagcggcgt atttgcgctt
     9841 tacggcggcg tgcagaccgc caaaacctat ggcgtcgacc tggcaattgg aaacgacgcc
     9901 ccggccggcc aggccgagga ggcgctgcgt tcaggccatg cgcaattgct gcgggcggtg
     9961 ccgcaaagcc atgtcgattt cctgcgcggg ctgatttttt gcgcgagctt cggcgacttc
    10021 ttcttctgcc atgccggcat ccgccccggc gtcgcgctgg acaggcagga ccgggaggac
    10081 ctgatctgga tacgcgaagc cttccacgat tatccgggcc tctacccgaa ggtgatcgtg
    10141 catggccaca cgccgcatcg gctgcccgag atcaaggcca accgcgtcaa tgtcgatacg
    10201 ctggcgcaca agtcaggccg gctcaccgcg ctcgtcgtcg acggcgcgaa gaaggaaatc
    10261 ctgacggtgg tggaagacgg gtcggttcag cgcagcgaag cggtgacgcc gtagccgtcg
    10321 acggcattgt gattgctggc cgcgtcggcg gcatagatgc cgagaccgca cagtacaatt
    10381 gctgaaacca tggcgaagga caggaacgca gctttcacgg tcgtcatctc ttgttgagct
    10441 tcctgcattc ctgcatgagg acggttacga ataagttgaa atggaagttc catttcaaca
    10501 gttgccgcag ctgaaccaag ttctcttgcg cctgttaccg ataagcaaca aaggggtttg
    10561 gcggcgcgga tgcccggcgg cgcacttcta ggagaagcgc cggacacaag ccaaggtcaa
    10621 aatgagcatc cgcggcggat ttggcgcggc gtgccaaaat tgcaacgacc ggcgcgtgat
    10681 gcgttcagaa tttcggatcg gggcgaccta ttcgcagacg atggcggtcg gcaacatccg
    10741 cgacgaggcc gtgtccaccg tctccaaaaa ttctggtatt gtcgaaattc gggacgaccg
    10801 gcacacgcat cgcaaccttg ccgcatgacg gcgccgcagg tcggcgccag catgccagca
    10861 taccaacaaa ggccgcatcc ccatcggaat tagccgagcg caaaaacggg aaagcgccat
    10921 gccactgcaa aaccgggtcg atcctttcgg cgtcatccat gccgtcgcgg cgcgcggact
    10981 gttcaccggc aatcgcggca tcatccacga tccggcgacc aagaagctgc tcaacaaacg
    11041 ctggacgctg aaggcgtgga tcatctgcac ctgcgaattc aagggccggc ggcgcgagcc
    11101 gatgggccgc aacaggccgg gaaagaacgg cgagccggcc ggggccggct ggaccgagct
    11161 tttcttcctg gatgaggtta cggcgctggc cgccggtcac cggccctgtt tctattgcca
    11221 acgcgaaagg gcggtggatt tcgtgcgccg cttcggcgag gctttcggcc ttgacgaacc
    11281 gcgcgcgccg atgctcgaca gcaggctgca cagggagcgg ctggcttcgg gcggcaggct
    11341 ccccaccatc gaggcgagcg agcttgccgg cctg
//
LOCUS       DASYCS010000442         3103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3612771,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000442 DASYCS010000000
VERSION     DASYCS010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3612771"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VGM46_13510"
     CDS             <1..573
                     /locus_tag="VGM46_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEY4193658.1"
                     /translation="FVDKASIQQIYLDTPYYLAPADKVSEDAFAVIRDAMAAKKKAGL
                     ARIVLYRRERPVVIEPLGKGMVLTTLRYDNTVRQPETVFDEIKAVKTDKEMIELAQHI
                     IDKKQAKFDPSKFDDKYEEALLELIRAKKAGKKAPKAKAAAKPSNVVNLFDALKKSLA
                     ADNDEAAATPAKGKAEPAAKKPAKSKRKSA"
     gene            574..3087
                     /gene="ligD"
                     /locus_tag="VGM46_13515"
     CDS             574..3087
                     /gene="ligD"
                     /locus_tag="VGM46_13515"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HEY4193659.1"
                     /translation="MAALEHYRAKRDFKKTSEPSGEAPRGRKAGEGGIFVIHKHAATR
                     LHYDLRLEHGGVLWSWAVTRGPSLDPHEKRLAVHVEDHPIDYAPFEGTIPKGEYGGGS
                     VIVWDEGTWTPDHDPAKGMKKGHIDFELRGEKLSGKWHLVRLKPRPGEKKDNWLLIKS
                     DDAAARPGEDILVDAPESVKSGLTVEEVGEGKTVGGKKPKVWHSNKPAHGKAASKATL
                     KLDFIEPALARLEADAPGGKEWLHEVKFDGYRMQAQIAGTEMHLLTRTGLDWTEKFVD
                     PVVAALGQLKCSDAIIDGEIVVLADSGVSSFSALQAALSEGRTDQMVYYAFDLMRLDG
                     EDLRGEPLIERKEKLRELLGDQPENSALRYSDHFVEPGKIMLEHACRMGLEGVVSKRA
                     DAPYRSGRGHSWIKSKCTQRQEFVIGGYLPSDKTGRGLRSLLVGYHEGGALHYAGRVG
                     TGFSGKVADDLKKRLDALKAPKSPFDAAVPKGKGLVWVKPDLVGEVEFRSWTQDRIIR
                     HASFQGLREDKPAEEVVQEKPKPSGKAGPKAQPASGDRPVPANAAKSSVKLTHPDKLL
                     WPDEKVSKQDLLDHYALVWKRMEKFVVNRPLALVRAPDGVGGQRFFQKHASAGMHDKI
                     FRMKDPEDDQELLFVKDFDGIAALVQMGVVEIHIWGSMVDELEKPDQIVFDLDPDEGV
                     SANKVREAALGIRARLEELSLPTFVKTSGGKGYHVVVPLQPAADWAAVKGFSHDFARA
                     MEQAEPDRYTATLSKKARTGKIFIDYLRNGKGSTTVAPYSSRAKKGATVSMPIMWQEV
                     HDGIAPNAFPIGDETTLRRFAQADPWKDFLASAKPLKLK"
BASE COUNT          673 a          929 c          981 g          520 t
ORIGIN      
        1 tttgtcgaca aagcctccat ccaacagatt tatctcgata cgccctatta cctcgcgcct
       61 gccgacaagg tctccgaaga cgccttcgcc gtcatccgcg atgccatggc ggcgaagaag
      121 aaagccgggc ttgcccgcat cgtgctttat cgccgcgagc ggccggtggt catcgaaccg
      181 ctcggcaagg gaatggtgct gaccacgctg cgctacgaca atacggttcg ccagcccgaa
      241 acggtgttcg acgagatcaa ggcggtgaag accgacaagg agatgatcga gcttgcccag
      301 cacatcatcg acaagaaaca ggcgaagttc gatccctcca aatttgacga caaatatgag
      361 gaagcgctgt tggagctgat ccgcgccaag aaggccggca agaaagcgcc aaaggccaag
      421 gccgccgcca agccttccaa cgtcgtcaat ctgttcgacg cgctgaagaa aagcctcgcc
      481 gccgataacg acgaggccgc cgctaccccg gccaagggca aggccgagcc tgccgccaag
      541 aagccagcca agtccaagcg caagtccgcc tgaatggccg ccctcgaaca ctaccgcgcc
      601 aagcgcgact tcaagaagac ctctgagcct tccggcgaag cgccgcgtgg caggaaggct
      661 ggcgagggcg gcatcttcgt cattcataaa catgccgcga cccgtcttca ctacgatctt
      721 cgccttgagc atggcggcgt cctgtggagt tgggcggtaa cgcgcggccc gagcctcgat
      781 ccgcatgaaa agcgcctggc ggtccatgtt gaggaccatc cgatcgacta cgcgcctttc
      841 gagggcacca tcccgaaggg cgaatatggc ggcggctcgg tcatcgtctg ggacgagggc
      901 acctggacgc ccgaccatga ccccgccaag ggcatgaaga agggccatat cgatttcgag
      961 ctgcgcggcg aaaaactgtc cggcaaatgg catctggtgc ggctgaagcc acggccgggc
     1021 gagaaaaaag acaattggct gctgattaaa tccgatgatg cggctgcccg tcccggcgag
     1081 gatattctgg tcgatgcacc ggaatcggtg aagtccggcc tcaccgtcga ggaggtcggc
     1141 gaaggcaaga cggttggcgg caaaaagccg aaggtctggc attccaacaa gcccgcccat
     1201 ggcaaagccg caagcaaagc gacgctgaag ctagatttca tcgagccggc gctggcgagg
     1261 cttgaagccg atgcgcccgg cggcaaggaa tggctgcacg aggtgaagtt cgatggctac
     1321 cgcatgcagg cgcagatcgc cggaaccgag atgcatctgc tcacccgcac agggctggac
     1381 tggacggaaa aattcgtcga cccggttgtt gcggcgcttg gccagctgaa atgcagcgac
     1441 gctatcatcg atggcgaaat cgtcgtgctg gccgacagtg gcgtgtcgtc gttctccgcc
     1501 cttcaggccg ccctgtcgga agggcgcacc gaccagatgg tctactacgc cttcgacctg
     1561 atgcggctgg acggcgagga tttgcgcggc gaaccgctca tagagcgcaa ggaaaagttg
     1621 cgcgaactgc tgggcgacca acccgaaaat tcggcgctgc gctacagcga ccattttgtc
     1681 gagccgggca agatcatgct cgaacatgcc tgccggatgg gcctggaggg cgtcgtctcc
     1741 aagcgcgccg acgcgcccta tcgcagcggg cgcgggcatt cctggatcaa gtcgaaatgc
     1801 acgcagcgcc aggaattcgt cattggcggc tatctgcctt ccgacaagac cgggcgtggc
     1861 ctgcgctcgc tgttggtcgg ctaccacgaa ggcggcgcgc tgcattatgc cggccgtgtc
     1921 ggcaccggct tttcgggcaa ggtggccgac gacctgaaga aaaggctgga tgcgctgaag
     1981 gcgccgaaat cgccattcga tgccgccgtg ccgaaaggca aggggctggt gtgggtcaag
     2041 ccggaccttg taggcgaagt ggagtttcgc agctggacgc aagatcgtat aatccgccac
     2101 gcatcctttc aggggttgcg cgaggacaag ccggcggagg aagtggtgca ggaaaagccg
     2161 aaaccatccg gaaaggccgg gccgaaggcc caacctgcgt ctggcgacag gccggttccg
     2221 gcaaatgcgg cgaaaagctc ggtcaagctt acgcatcccg acaaacttct atggccggat
     2281 gaaaaagtgt cgaagcagga tttgctcgac cattacgcgc tggtctggaa gcgcatggaa
     2341 aagttcgtcg tcaaccggcc gctggcgctg gtgcgcgcgc ccgatggcgt cggtggccag
     2401 cgcttcttcc agaaacatgc ttctgccggc atgcacgaca agattttccg catgaaggac
     2461 cccgaagacg atcaggaact gctgttcgtc aaggatttcg acggcatcgc cgcgctcgtt
     2521 cagatggggg tcgtcgaaat ccacatctgg ggcagcatgg tcgacgagct tgaaaagccg
     2581 gaccagatcg tcttcgatct cgatcccgac gagggcgtca gcgccaacaa ggtgcgcgag
     2641 gcagcgcttg gcattcgcgc acggctggag gaattgtcgc tgccgacttt cgtcaagacc
     2701 tctggcggca agggctatca tgtcgtggtg ccgttgcagc cggcggccga ttgggcggcg
     2761 gtgaaaggct tttcgcacga tttcgcccgc gccatggagc aggccgaacc cgaccgctac
     2821 accgcgaccc tgtcgaaaaa ggcgcgcacc ggcaaaatct tcatcgacta cctgcgcaac
     2881 ggtaagggtt ccaccacggt tgccccctat tcgtcgcgcg ccaagaaggg tgcgacggtt
     2941 tcaatgccga taatgtggca ggaagtgcat gacggcatcg cgcccaacgc ctttcccatc
     3001 ggggacgaga cgacgctgcg gcggttcgca caagccgatc cctggaagga cttcttggcc
     3061 agtgccaagc cgctgaagct gaaatagctg gagcatttcc agc
//
LOCUS       DASYCS010000443        24122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1146879,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000443 DASYCS010000000
VERSION     DASYCS010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 24122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24122
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1146879"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1132
                     /locus_tag="VGM46_13520"
     CDS             <1..1132
                     /locus_tag="VGM46_13520"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEY4193660.1"
                     /translation="DLNVPVTDGKVSDTTRIERILPTLDELSKKGAKVILLAHFGRPK
                     GGPAPEFSLDPIANAVSAMIGRPVAFAADCIGAEAANAVGAMKNGDILLLENTRFHKG
                     EEKNEPDFADGLAKNGDIYVNDAFSAAHRAHASTEGLARRLPAFAGRTMQAELDALEK
                     GLGNPAKPVVALVGGAKVSTKIDLLMNLVRKVDALVIGGGMANTFLAARGTAVGKSLC
                     EHDLAATARQIMIEAATAGCAIILPSDGVVAREFKAGAENQTVAIEACPPDAMILDAG
                     EKTVDTINQWIDRAATLVWNGPLGAFEIEPFDRATVAVARHAAARTKAGKLVSVAGGG
                     DTVAALNHAGVVGDFTYVSTAGGAFLEWMEGKPLPGVDVLKN"
     gene            1254..2279
                     /locus_tag="VGM46_13525"
     CDS             1254..2279
                     /locus_tag="VGM46_13525"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HEY4193661.1"
                     /translation="MSERLEDIAARIVAGGKGLLAADESSGTIKKRFDAIGVQSTADS
                     RRDYREMMFRTTEAMTNCISGVILYDETIRQKAADGTPLIDIIRAAGAVPGIKVDVGA
                     KPLAGFAGETVTEGLDGLHERLADYYKIGARFAKWRAVVDIDTAKGVPSGGAIASNMH
                     ALARYAALCQEAGIVPIVEPEVMMEGSHDIDTCYEVSKAALLKLYAELHGMRVRLEGT
                     ILKPNMVISGKKSATQAGPEEIAEKTIRLFRRTVPVAVPGIAFLSGGQSDVEATENLN
                     AVNAIGPHPWKITFSYGRALQAAAQKAWSGKASNVAAGQAAFDHRARMNHLAALGQWK
                     PSLEEAG"
     gene            complement(2353..2514)
                     /locus_tag="VGM46_13530"
     CDS             complement(2353..2514)
                     /locus_tag="VGM46_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006200766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193662.1"
                     /translation="MSKMLALVILAMMVIQLIKPLGWPGLRARRDFWKLAVLALAAIS
                     VTAVLGHWG"
     gene            2639..4546
                     /locus_tag="VGM46_13535"
     CDS             2639..4546
                     /locus_tag="VGM46_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA synthetase"
                     /protein_id="HEY4193663.1"
                     /translation="MVSRYHEVYESWQRDPEAFWAQAAEEIDWIKPFDKVFDPGAGVY
                     GQWYTGGLCNSCWNAVDRHVAGGRAEQTALIHDSAYTGAIRKFTYAEMKREVIALASV
                     LKNQGVGKGDRVIIYMPMVPEAVFAMLACARLGAIHSVVFGGFAAQELATRIDDAKPK
                     LVLSASCGLEPGRMVAYKPLLDRAIELARHKPERCIILQREQLRCELKENYDIDFADA
                     VARERAAGANVDCVPVLATDPLYILYTSGTTGQPKGVIHDNGGHMVSLKWSMENENGV
                     KPGEVFWSASDIGWAVGHSYIVYGPLLHGCTTVMFEGKPVGTPDASAFWRVISQHGVV
                     GLYTAPTTFRAIKGQDPKGELVARFDLSKFRMLYLAGERADPETIKWAEQKLGVPVLD
                     NWWQTETGSPITHNPVGLGLLPVKHGSPGVPMPGYDIRVLDDAGHELPRGTLGNVVIK
                     LPLPPGCLPTLWNADARFHQAYLDEFPGYYKTADAGIVDEDGYLFIMARTDDIINVAG
                     HRLSTGAMEEVLAAHPDVAECAVVGIADSLKGQVPLGFVVLNAGVTRAPALIEMEAVS
                     LVRDRIGPVAAFKTAITIKRLPKTRSGKILRGTMQKIADRQEWTMPATIDDPAILDEV
                     AAAMAERGIGG"
     gene            4696..5955
                     /locus_tag="VGM46_13540"
     CDS             4696..5955
                     /locus_tag="VGM46_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019995063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEY4193664.1"
                     /translation="MAAAFCGVDFGTTNSTVGLCVPGGIPALIPLEADHVTIPSALFY
                     SLEDGKTYFGRAAVFEYMDGAEGRFMRALKSILGSSLMKDTTQVGRERLRFEAIIGRF
                     LRHLRGRLEAEHGQAPEQVVLGRPVRFVDEDDEADAQAQAQLEAAARMEGFRHIEFQF
                     EPVAAALNHERALNGEQLALVVDIGGGTSDFSVLRLSPERARQADRLSDILSTTGVHV
                     GGTDFDKQLNIAKVMPHLGLGSTTLDGKRQLPVWYFNDLATWHRINTLYTQKNGRDIQ
                     ALTREAAEPQKLERYAHVLKNRSGHRLAGEVEKAKIALTDDHAGIIALREPGLALEVP
                     VTRGEFEAASADLVSKIGASIEDALRLAGIQAAAIETVILTGGGVQVPVVEMAAVSRF
                     PGARIARSDRFGSVGLGLAIDAARRFG"
     gene            6042..6233
                     /locus_tag="VGM46_13545"
     CDS             6042..6233
                     /locus_tag="VGM46_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006327964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193665.1"
                     /translation="MTHFTMLHAMVRYVGKVRTLRNEIRTERFLNALPSDIRADIGWP
                     DQHEGWPSRHDRFSQGQAR"
     gene            6386..7105
                     /locus_tag="VGM46_13550"
     CDS             6386..7105
                     /locus_tag="VGM46_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HEY4193666.1"
                     /translation="MRRADRLFQIVQHLRGGRLVTAHKLGTWLEVSERTIYRDIADLQ
                     STGVPIDGEAGVGYLMREGFDLPPLMFTRDEIVALVAGARMVRAFGGAAMARAAEEAL
                     VKIGAVLPEAEKDRIARTEIHTPMWVVSDAARDAIDLIERAVEKRQVLTLDYSDEAGR
                     GSVRDVRPLGLWFWGKVWTLIAWCEMRGDFRAFRIDRIASVVLAGRVFKPERGKQLGD
                     FYRAVERSEGYGAAPDRARGS"
     gene            complement(7197..7895)
                     /locus_tag="VGM46_13555"
     CDS             complement(7197..7895)
                     /locus_tag="VGM46_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1013 domain-containing protein"
                     /protein_id="HEY4193667.1"
                     /translation="MANTLLMPKATAVWLVDNTALSFEQIAQFCGLHHLEVKAIADGE
                     AAQGIKGLDPIMTGQLTRDEIARAEKDGNLRLKLSEPKVRVPESKRKGPRYTPLSKRQ
                     DRPNAILWLVRNHPELKDAQISRLVGTTKSTIEQIRDRKHWNAANLVPMDPVTLGLTS
                     QIDLDMEVNRASRGKEAAAPVGDTLLPAALTERLVPAPEKPKDENEELDANAVFAKLS
                     ALRSKDQDTDEDDE"
     gene            8110..8343
                     /locus_tag="VGM46_13560"
     CDS             8110..8343
                     /locus_tag="VGM46_13560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193668.1"
                     /translation="MLSKHDRYSHITRTRLPVRVAASQASGGVEPDGAAAGAARRFAD
                     RTLGLPGDCGCGGTGKVSGGGGMAIDGSAGARI"
     gene            8683..12894
                     /locus_tag="VGM46_13565"
     CDS             8683..12894
                     /locus_tag="VGM46_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY4193669.1"
                     /translation="MVIRDDSGVKPPARPANIRASARKAGSPPFLPAESLLALVEAGS
                     DWLWETDADLRFSWVSENYQKITGIEAAQVLGRFRFDFLSPASQGSELVASHLEDLQH
                     HRPFRDFVYELKGSRQDCRWISTTGFPRFDAEGNFIGYRGLGRNVTEIAGTLGKLEKA
                     APHADRAELLASAERNRQLLFDIERTIDAVEMGIVVVDANLNTEIINKAFYKIWDMTP
                     QQIALGSPFRKLIESGRSGGLEDIRDEDWESYVARRLNEVRAGDLAPREFRRKNGRTT
                     IYAITALSGGKRLVSYYDLTELKKQEAQVAAANARTEEAFCNLRIMVDSIPIGIIVVD
                     ADMRAEIVNRAFYDFWRLDPASIGIGASFRELMEANRTIDPHGFDAEAWPHYIAKREA
                     EIRAGVAEVRQLTRGDGRTLISSTTPLSGGKRLISYVDVTDMKNREAELAEALEKAKL
                     AEAVINSVPNPMFVKDADLNFVMANQAFADFFGTTPDQILGRKDDDLVPPEERGMFEE
                     SERQVLATGELYETEAQYVRDGFHGTRVVRKNRFETTSGKIYLASSLFDVSESKRREK
                     EAEEAHRHLTNVLESLPAGVAVYDRDDKFLFANEKFRETLPDLRAAWTPGSTLREALA
                     IGHAHGYFRDNGDKAIDGLYDCDVEGWLDAMENHYRQRDAVYERVTPEGRWYQVYNKR
                     TDDGTFIGVRVEITDIKERERALEESLRENEVFRSLIDNVPVSIYAKRADLRQFYVNK
                     GWCDLTGYSKQEAIGKTDIEIFGADGEAFVESDLSVLRTGETQEIEETVVSADGSRRH
                     QFARKGAMIASDGSLYLIGSTTDITELKQREAELQEARQRAVLADRAKSEFLANMSHE
                     IRTPMNGVLGMAELLAKSDLDPKQKTFTDIIVKSGNALLTIINDILDFSKIDAGQLVL
                     DPAPFSLAEAIEDVATLVSTRAREKDLELIVRIEPGFDGLFVGDVGRVRQIVTNLLGN
                     AVKFTDEGHVLVDVTGEHAPGGTRLHISVTDTGIGIPEEKRALVFEKFSQVDASSTRR
                     HEGTGLGLAITSRLVDLMGGEIGVESAEGRGSTFWFTITLATAERAGKPRITPIDVTG
                     ARVLVVDDNAVNRSILAEQMASWNFDSCAAASGEEGLKVLIAAAAYGVPVDCIVLDYQ
                     MPGMTGTEMARIVRNTDSLADTPIVMLTSVDQSLAQASYRDLGIDAHLIKPARSSALL
                     ETLVTTIQRHRAKTMPLGATAFEESAASCAPTPAIDRQAGDAAGNALRQPGAIRPRSA
                     PVAGEGRRLDILVAEDNEVNQLVFTQILGETGYSFEIVGNGRKALDAFARLKPLMILM
                     DVSMPEMNGLEATQEIRRIEQDAGTHVPIVGVTAHALKGDRERCIEAGMDDYLSKPIS
                     PKALMEKLERWLGTDHEAQWAGTAG"
     gene            13103..14161
                     /gene="pstS"
                     /locus_tag="VGM46_13570"
     CDS             13103..14161
                     /gene="pstS"
                     /locus_tag="VGM46_13570"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEY4193670.1"
                     /translation="MRHFIRSAVVAVAMATASTLTLSTAFAADISGAGSTFIYPVFAK
                     WADTYKKDTGIGLNYQSIGSGGGIKQVVAKTVTFGATDKPMSDEELEKNGLTQFPMVM
                     GGIVPIVNLEGVKPGELILDGKTIADIYMGAITTWDDAAIKALNPDLTLPSTAIAVVH
                     RSDGSGTTFNFTDYLGKLSPDWKEKVGSDTAVEWPTGVGAKGSEGVANTVKQTDGGIG
                     YVEYAYAAQNDLSYAKMMNAAGKVVSPSLESFGAAAANADWKGAKNFNVIITNEPGDE
                     TWPIAASTWVLIHKAPDDAVAAGEALKFFAWAYQDGKGTAKDLDYVSIPDSVVDMIKA
                     SWTADIAADGKPVYTVGQ"
     gene            14247..15230
                     /gene="pstC"
                     /locus_tag="VGM46_13575"
     CDS             14247..15230
                     /gene="pstC"
                     /locus_tag="VGM46_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEY4193671.1"
                     /translation="MSAVQEALPAFKGSRAATVRRFALTDTIFHALTKASAILVLLLL
                     GGVGISLVAGSWPALSTFGFSFLVSESWNPVKENFGALAPIYGTVVTSAIAILIAVPI
                     GIGIAIFLTELCPRPLRRPIGMAVELLAGIPSIIYGIWGLFVLAPFLQRTIQPFIIGA
                     FKNVPGLNGLFAGPPYGIGLLTSALILAIMILPFITSITKDVFDTVPPVLKESAYGIG
                     CTTWEVTRRVVIPYTRVGIMGGVMLGLGRALGETMAVTFVIGNAHRISESLFAPATTI
                     SATIANEFTEAVGDLYTSSLVALGLILFVITFLILALARYMLMRIDARTGA"
     gene            15233..16078
                     /gene="pstA"
                     /locus_tag="VGM46_13580"
     CDS             15233..16078
                     /gene="pstA"
                     /locus_tag="VGM46_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEY4193672.1"
                     /translation="MAVTSSLHSRRKMANSIMMTLCVVAAGIGLAWLALILGALVYKG
                     ASGLSLAVFTQMTPPPGDAGGLLNAIFGSVVMTVIAVLVGTPIGVLAGTYMAEYGRFS
                     RLTTVVRFINDILLSAPSIVIGLFVYELMVRPMGHFSAIAGAVALAILVIPVVVRTTE
                     DMLNLVPNALREAGTAIGAPRWVVIKSVAYRAAMSGIVTGILLSIARISGETAPLLFT
                     ALNNQFWSGNLNAPMASLPVTIFQFALSPYEEWQQLAWTGALIITLTVLVLSIIARSL
                     TGRRT"
     gene            16081..16893
                     /gene="pstB"
                     /locus_tag="VGM46_13585"
     CDS             16081..16893
                     /gene="pstB"
                     /locus_tag="VGM46_13585"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEY4193673.1"
                     /translation="MAQMLAPEMTMTDDTAGKAGKTKIEVKNLNFYYGQSKALKDITL
                     SLPERSVTAFIGPSGCGKSTLLRVFNRIYELYPKQRAEGHVMLDGQNVLDRSQDLNLL
                     RTKIGMVFQKPTPFPMSIYENIAFGVRLYEKIGKSEMDGRVEQALRRAALWSEVKDKL
                     NASGLSLSGGQQQRLCIARTVAVKPEVILLDEPASALDPLSTAKIEELIDELQADYTI
                     VIVTHNMQQAARVSKQTAFMYLGELIEFDKTEKIFTSPGHKRTQDYITGRFG"
     gene            16930..17637
                     /gene="phoU"
                     /locus_tag="VGM46_13590"
     CDS             16930..17637
                     /gene="phoU"
                     /locus_tag="VGM46_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEY4193674.1"
                     /translation="MTEHTVAAFDDDLGQIGRLIQGMADLAGAMVSGATHALLTADNA
                     QAQRIVSDDALMDARQRELDERAITLIARRQPMADDLRSVVGAIRMAGDLERIGDLAK
                     NIAKRVAAVGESAVPRELSHSIDGMAQLVLEQVRGVMAHYSAQDAAGLITLRDDDERI
                     DVKYTSVFRELLTYMMEDPRNITACTHLLFCAKNLERIGDHVTNIAENAYYVMTGQQL
                     PAHRPKSDEMVMSAPAA"
     gene            17759..18445
                     /gene="phoB"
                     /locus_tag="VGM46_13595"
     CDS             17759..18445
                     /gene="phoB"
                     /locus_tag="VGM46_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HEY4193675.1"
                     /translation="MAPRIMVVEDEEPLGVLLRYNLESEGYHVEVVTRGDEAEIRLQE
                     NVPDLLVLDWMVPAVSGIELCRRLRMRSETERLPIIMLTARGEESDRVRGLSTGADDY
                     LVKPFSTPEFMARVKALLRRAKPEVLSSVLKVGDIVLDRETHRVYRDKSEIRLGPTEF
                     RLLEFMMRSPGRVFSRGQLLDNVWGETIYVDERTVDVHVGRLRKAVNDGHAQDVIRTI
                     RGAGYAIKEE"
     gene            complement(18560..19117)
                     /locus_tag="VGM46_13600"
     CDS             complement(18560..19117)
                     /locus_tag="VGM46_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011426984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEY4193676.1"
                     /translation="MTVAVSADGLPALVLNADYRPLSYYPLSLWSWQDAIKAVFLGRV
                     NIVAEYEHAVSSPTFSMKLPSVVSLKAYVKPSRHPAFTRFNVFLRDRFQCQYCGTPED
                     LTFDHVIPRRCGGATTWENVVAACSPCNLTKGGMMPAQAKMFPRQKPYQPTVHDLHNN
                     GRLFPPNHLHESWMDYLYWDVELEP"
     gene            complement(19246..19887)
                     /locus_tag="VGM46_13605"
     CDS             complement(19246..19887)
                     /locus_tag="VGM46_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006199899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEY4193677.1"
                     /translation="MQHIATLDDIERGLDALGVLDPRLLAVRAMVGEVPLRLSEPGFR
                     SLASIVVSQQVSRASADAIFGRLVKIVDPLTPQAILAADDAVFRQAGLSRPKQRGLLA
                     VAQAVADGLDLYHLCRLDADEAMKLLLAVPGIGPWTAQVYLLFAAGHPDIFPARDVAL
                     QTAVGHALGIAPRPPEKTLIAIAESWSPWRGVAARLFWAYYRQLKGREAAPPA"
     gene            19963..20838
                     /gene="gluQRS"
                     /locus_tag="VGM46_13610"
     CDS             19963..20838
                     /gene="gluQRS"
                     /locus_tag="VGM46_13610"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEY4193678.1"
                     /translation="MSLPTFRFAPSPNGELHLGHAYSALLNQKLAKAAGGRLLLRIED
                     IDTVRCTPEFEAAIFNELNWLGVGWEKPVRRQSQHFAEYAEILNRLIGEGLVYPAFMS
                     RGEIRAFITDRQKRGLDWPRDPDGVPLYPGADRALPDAQRKRRIAEGEPYAWRLDTAA
                     AIARIGRPLSWMEHADENLDTASPVEARPQDWGDVILARRDVPTSYHLAVVADDALQG
                     VSHVVRGRDLFLATSLQRLLQELLGLPQPVYCHHRLILGSDGRKLSKSLAGTSLAELR
                     RGGATAHDIRNMPGL"
     gene            20959..21849
                     /locus_tag="VGM46_13615"
     CDS             20959..21849
                     /locus_tag="VGM46_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006199895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY4193679.1"
                     /translation="MHSLKRFIPAIFVVLWATGFIGARYAMPWVEPFTFLSMRFVIAT
                     ILLAAIAVALGLKRATRKEALNAAIAGALMHGVYLGGVFWAIHRGMPAGLSALIVGLQ
                     PLVTAVMAGKLLGERILPRHWVGLATGFAGVVIVLLPKLGELGSGIDAQTVSACIFAV
                     FGITLGSIWQKRFALSGNLFATTVWQYVGGALVTVPAALLFETRSVVFNGELAFALAW
                     LVLVLSIGAVFLLMVMIREGEMSKVASVFYLVPAVTAVMAWALFGETLDLIQIAGMAI
                     TTFGVALATAQSGTRARASR"
     gene            complement(21816..22670)
                     /locus_tag="VGM46_13620"
     CDS             complement(21816..22670)
                     /locus_tag="VGM46_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEY4193680.1"
                     /translation="MLRNVVAFKRVVYDALGHFNNDDGWAMSSHLAITALMALFPFLI
                     FATTLASFLGAQAFADTAVHLVFDTWPEQIAKPIAREVLNVLTVQRTDLLTYGVLLAA
                     YFASNGIEALRTSLNRAYRVVETRGIIYRRAQSLFFVIIATIGFLIISVLLVLAPILA
                     RLAEAEFEWIKPYMGTITLWRYIIASTVIVGGLFAVHYWLPIGKRRFLSLIPGIVFTL
                     IGWLIGSTIFATYLDHFSSYVTTYAGLASIMIAVVFLYIVSAIFILGGEFNAAIIRYL
                     EARARVPD"
     gene            22757..23584
                     /locus_tag="VGM46_13625"
     CDS             22757..23584
                     /locus_tag="VGM46_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018429664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY4193681.1"
                     /translation="MKELRTIIVTGASSGVGAYCARALKADGWRVFATARKEEDIAAL
                     ETAGIEAFPLDYRDPASIEALVAAVLERTGGSLDALYNNGAYAQAGAVEDVPVELLRQ
                     QFEANFFGWHDLTRRVMPAMRRQGHGRIVNCSSILGLVPLPYRGAYCASKYALEGLML
                     SMRQELAGSGIHVSLIEPGPIASDLHDNGLPWFRDSIDLENSVHREAYRRQMDELWSG
                     PPRAKAGPVVVYTALKHALLSRRPRPHYVVTMPARIGVVLKRILPASLFYRLLARST"
     gene            23601..23792
                     /locus_tag="VGM46_13630"
     CDS             23601..23792
                     /locus_tag="VGM46_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HEY4193682.1"
                     /translation="MGTVFNILAVIVMVAVVAVLLRGLFNMMRGGDGMTSNKLMQARV
                     LLQFVAVVLIMLAVYFTRK"
     gene            23819..>24122
                     /locus_tag="VGM46_13635"
     CDS             23819..>24122
                     /locus_tag="VGM46_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006265284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP:cob(I)alamin adenosyltransferase"
                     /protein_id="HEY4193683.1"
                     /translation="MVKLNKIYTRTGDDGTTGLGTGERRLKSDLRVEAYGTVDEANAC
                     IGMARIHTAKTYPAIDAMLGRIQNDLFDLGADLAVPDSGEPLDYEPLRITAAQVERL"
BASE COUNT         4536 a         7471 c         7782 g         4333 t
ORIGIN      
        1 cgatctcaac gtgccggtga ccgatggcaa ggtcagcgac acgacccgta tcgagcgcat
       61 tcttccgacg ctcgacgaat tgtcgaaaaa aggcgccaag gttatcctgc tggcccattt
      121 cggccgcccg aaaggcgggc cggcgcccga attctcgctc gacccgattg ccaacgccgt
      181 ctcggcgatg atcggcaggc cggtcgcctt tgccgccgac tgcatcggcg cggaagccgc
      241 caacgcggtc ggcgcgatga agaacggcga catactgctc ctggaaaaca cacggttcca
      301 caagggcgag gaaaagaacg aaccggattt cgccgacggg ctggcgaaaa acggcgatat
      361 ctacgtcaac gacgcgtttt cggcggcgca tcgcgcccat gcctcgacgg aagggctggc
      421 gcgtcggctg ccggcctttg ccgggcgcac catgcaggcc gaactcgacg cgctggaaaa
      481 gggcctcggc aacccggcca agccggtggt ggcgcttgtc ggcggcgcga aagtatcgac
      541 caagatcgac ctgctgatga atctggtgcg caaggtcgat gcgctggtca tcggcggcgg
      601 catggccaac acgtttctcg ccgcgcgcgg caccgctgtc ggcaagtcgc tgtgcgagca
      661 cgaccttgcg gcgaccgcca ggcagatcat gatcgaggcg gcgacggccg gctgcgccat
      721 tatcctgccg tcagacggcg tcgtcgcaag ggaattcaag gccggggcgg aaaaccagac
      781 cgtggcgata gaggcctgcc cacccgacgc catgatcctg gacgccggcg aaaagaccgt
      841 cgacaccatc aaccagtgga tcgaccgcgc cgcgacgctg gtgtggaacg ggccgctcgg
      901 cgctttcgag atcgagccat tcgaccgggc gacggtggcc gtggcaaggc atgctgcggc
      961 gcggaccaag gccggcaagc tcgtgtcggt ggccggcggc ggcgatacgg tggcggcgct
     1021 gaaccatgcc ggcgtcgtcg gtgatttcac ctatgtctcg acggcaggcg gcgcattcct
     1081 cgaatggatg gaaggcaagc cgctgcccgg cgtcgatgtg ctgaagaatt gaattgtcct
     1141 ttcgaggcgg cgctggatgg cgcgctgcac ctttcaatcg attttgactt ttgaccgcgt
     1201 ttgttttggg gctagcatca tcgacgtcga tggtcggccc gcaggagagc gaaatgagtg
     1261 aacgtctcga ggacattgcc gcgcgcatcg tcgccggcgg caagggcctg ttggccgccg
     1321 acgaaagctc gggcaccatc aagaagcgct tcgacgcgat cggcgtgcaa tccaccgccg
     1381 acagccgccg cgactatcgc gagatgatgt tccgcaccac cgaggcgatg acgaactgca
     1441 tttccggcgt cattctctac gacgaaacga tccgccagaa ggccgccgac ggaacgccgc
     1501 tgatcgatat catcagggcc gccggcgccg ttcccggcat caaggtcgat gtgggggcga
     1561 agccgctggc gggctttgcc ggcgaaacgg tcaccgaggg gctggatggc ctgcacgagc
     1621 gcctggcgga ctattacaag atcggcgcgc gcttcgccaa atggcgcgcg gtggtcgaca
     1681 tagacacggc caaaggcgtg ccgtccggcg gggcgatcgc ttccaacatg catgcgctgg
     1741 cgcgctatgc ggcattgtgc caggaagccg gcatcgtgcc gatagtcgag ccggaagtga
     1801 tgatggaagg cagccacgac atcgacacct gctatgaggt ttcgaaagcg gcgctgttga
     1861 agctttatgc ggaactccat ggcatgcggg taaggctgga aggcacgatc ctgaagccga
     1921 acatggtgat ttcgggcaag aaatccgcca cgcaggccgg gccggaggaa atcgccgaaa
     1981 agacgataag gctgtttcgc cggaccgtgc cggtggcggt gcccggcatc gcctttcttt
     2041 ccggcggcca atcggacgtc gaggcgacgg aaaacctcaa cgccgtcaat gccattggtc
     2101 cgcatccgtg gaagatcacc ttttcctatg gccgggcttt gcaggcggcg gcgcaaaagg
     2161 cctggagcgg caaggcgtcg aatgtggcag ccggccaggc agcgttcgac catcgcgccc
     2221 gcatgaacca tctggcggcg cttggccagt ggaaaccctc gctggaagaa gcgggctgat
     2281 cggccagccg tttaattgcc cgtcttgaag tcggggagcg tgctttggaa tgaagcggcg
     2341 ctcccttttc tttcaccccc aatggccaag aacggcggtc accgagatgg cggccagcgc
     2401 cagcaccgcc agcttccaga agtcgcgccg cgcccgcagg ccgggccagc ccagcggttt
     2461 gatcaactga atgaccatca tggcgaggat gacgagggcg agcattttcg acatgccgcc
     2521 tttgaccagt atcgtgcgct tctgtcaaag ccagcaaggc ttggccatgt ggcgagatgg
     2581 acggagcgcc atttgaagag ctagaaagac gcctgcaaga acgaacggga ggaacggcat
     2641 ggtttcgcgc taccacgagg tctatgaaag ctggcaacgc gaccctgagg ccttctgggc
     2701 gcaggccgcc gaagagatcg actggataaa gccgttcgac aaggtgttcg atcccggtgc
     2761 tggggtctac ggccagtggt atacgggagg cctgtgcaat agctgctgga acgcggtgga
     2821 ccgtcatgtc gccggcgggc gggccgagca gaccgcgctg atccacgaca gcgcctatac
     2881 cggcgcgatc cggaaattca cctatgccga gatgaagcgc gaggtcatcg cccttgccag
     2941 cgtgctgaag aaccagggcg tcggcaaggg cgaccgcgtc atcatctata tgccgatggt
     3001 gccggaagcg gtgttcgcga tgcttgcctg cgcgcgcctc ggcgccatcc attcggtggt
     3061 gttcgggggc tttgccgcac aggagctggc cacccgcatc gacgatgcca agcccaagct
     3121 cgtcctgtcg gcctcctgcg ggctggaacc cggccggatg gtcgcctata agccgctgct
     3181 cgacagggcc atcgaactgg cccgtcataa gcccgagcgc tgcatcatcc tgcaacgcga
     3241 gcaattgcgc tgcgagctga aggaaaatta cgacatcgat ttcgccgatg cggtggcgcg
     3301 cgagcgggca gcgggcgcaa atgtcgattg cgtgccggtg ctggcgaccg atccgcttta
     3361 tatcctctat acgtcaggca ccaccggcca gcccaagggc gtcatccacg acaatggcgg
     3421 ccatatggtt tcgttgaaat ggtcgatgga aaacgaaaac ggcgtcaagc cgggcgaagt
     3481 gttctggtcg gcatccgata tcggctgggc ggtcggtcat tcctacatcg tctatggtcc
     3541 gctgctgcat ggctgcacca cggtgatgtt cgaaggcaag ccggtcggca ccccggatgc
     3601 gagcgccttc tggcgcgtga tttcgcagca tggcgtagtg gggctgtaca ccgcgccgac
     3661 gaccttccgg gccatcaagg ggcaagaccc gaaaggcgag cttgtcgcca ggttcgacct
     3721 ttccaaattc aggatgctgt atctcgcggg cgagcgcgcc gaccccgaaa ccatcaaatg
     3781 ggccgagcag aagctgggcg tgccggttct ggacaattgg tggcagaccg aaaccggttc
     3841 accgatcacc cacaacccgg tgggccttgg cttgctgccg gtgaaacacg gctcgccggg
     3901 ggtgccgatg cccggctacg acattcgcgt gctggacgat gccggacacg aactgccgcg
     3961 cggaacgctt ggcaacgtcg ttatcaagct gccgctgcca cccggctgcc tgccgacctt
     4021 gtggaacgcc gatgcgcggt tccatcaagc ctatctcgat gaattcccag gctattacaa
     4081 gacggcggat gccggcatcg tcgacgagga cggctatctc ttcatcatgg cgcgcacgga
     4141 cgacatcatc aacgtcgccg gacaccgcct gtcgacgggt gcgatggaag aggtgctggc
     4201 ggcgcatccg gatgttgccg aatgtgcggt cgtcggcatc gccgatagtt tgaaaggcca
     4261 ggtgccgctc ggcttcgtgg tgctgaatgc gggcgtcacg cgcgcgccgg cgctcattga
     4321 aatggaagca gtgagcctgg tgcgcgaccg gatcgggccg gttgccgcgt tcaaaacggc
     4381 gatcacgatc aagcggctgc ccaagacgcg ctcgggaaag atcctgcgtg ggaccatgca
     4441 gaagattgcc gacaggcagg aatggacgat gccggccacc atcgacgatc cggcaatcct
     4501 tgacgaggtt gcagcggcga tggccgagcg cggaattgga gggtaaggca gggcggcttg
     4561 acgacccggc ctggatggcg gccgtgacga cacgcggcct ttccttttca gctgcgatcc
     4621 gctagacggc ggctttcctg aaagcgcccg tcatgcagcc cagctgccgg gctttccatt
     4681 gcgagcattc atcccatggc agcagccttt tgtggcgtcg atttcggcac caccaactcg
     4741 accgtcggcc tatgcgttcc cggcggaata ccggccctta tccctctcga agcagaccat
     4801 gtgaccattc cctccgcgct gttttacagc ctggaagacg gcaagaccta tttcgggcgc
     4861 gccgcggtat tcgaatatat ggatggcgcg gaggggcgct tcatgcgcgc gctgaaatcc
     4921 atcctcggca gcagcctgat gaaggacacc acccaggtcg ggcgcgagcg cctgcgcttc
     4981 gaggcgatca tcggccggtt cctgcgccat ctgcgcggcc gcctcgaagc cgagcacggg
     5041 caagcgcccg aacaggtggt gctggggcgc ccggttcgct tcgtggacga ggatgacgag
     5101 gccgacgcgc aggcgcaggc gcagctggaa gcggccgcgc gcatggaagg ttttcgccac
     5161 atcgaattcc agttcgagcc ggtggcggcg gcgctgaacc acgaacgcgc gctcaatggc
     5221 gagcaactgg cgctggtggt cgacattggc ggcggcacct cggacttttc cgtgctgcgg
     5281 ctgtcgccgg agcgcgcgcg ccaggcggac cgcctgagcg acattctaag cacgaccggc
     5341 gtgcatgtag gcggcaccga cttcgacaag cagctcaaca tcgccaaggt catgccgcat
     5401 ctggggcttg gctcgaccac gctcgacggc aagcgccaac tgccggtgtg gtacttcaac
     5461 gacctggcga cctggcaccg catcaacacg ctctatacgc agaagaacgg gcgcgacatc
     5521 caggcgttga cccgcgaggc ggcggaacct cagaagctgg agcgctatgc gcatgtgctg
     5581 aagaaccgct ccggccaccg gctggccggc gaggtggaga aggccaagat cgcgctgacc
     5641 gacgaccatg ccgggatcat cgcgctgcgg gagccgggcc ttgcgctcga agttccggtg
     5701 acgcggggag aattcgaggc ggccagcgcc gacctcgttt cgaagatcgg cgcctccatc
     5761 gaggatgcgc tgcgtctcgc cggcatccag gcggcggcaa tcgaaaccgt cattctgacc
     5821 ggtggcggcg tgcaggtgcc ggtggtggaa atggcggcgg tttcgcgctt tcccggcgcg
     5881 cggattgccc gctccgaccg cttcggttcg gttggtctcg gcctcgccat cgacgcggcg
     5941 cggcggttcg gctgaatatc gccggagtgt cgccggcctc ctgacagact gctgtcagga
     6001 ggggtgtgcg atgatggctt ccaatcaagg aggaggccac gatgacccat ttcacgatgc
     6061 tgcacgcgat ggtgcgttat gtcggcaagg tccgcacctt gcgcaatgaa atccgcaccg
     6121 agcggttcct gaacgcgctg ccttcggata ttcgtgccga tatcggctgg cccgaccagc
     6181 atgaaggctg gccaagccgc catgaccggt tttcccaagg gcaggcccga tagcaggcca
     6241 ggcgactgcc ggatgcgcgc gccgcgtcgt tatcttgctt ccacgtccca tgtccggcgc
     6301 gatccgctcc tgacactatg ctgtcaggag catcatgcga tactatgctt cctgcgcctt
     6361 gggcggacgg gcgagaggaa cgacgatgcg acgcgccgac aggcttttcc agatcgtaca
     6421 gcacctgcgc ggaggcaggc tggtcaccgc gcacaagctg gggacatggc tcgaagtttc
     6481 cgagcgcacc atctaccgcg acatagccga cctgcaatcg accggcgtgc cgatcgatgg
     6541 agaggcgggc gtcggttacc tgatgaggga gggtttcgac cttccgccgc tgatgttcac
     6601 gcgtgacgaa atcgtggctc tggtcgcggg cgcgcgcatg gtgcgcgctt tcggcggggc
     6661 cgccatggcg cgcgcggcgg aggaagcgct ggtgaagatc ggcgcggtat tgcccgaagc
     6721 cgaaaaagac cgcatcgccc gcaccgaaat ccacacgccc atgtgggtgg tgagcgacgc
     6781 cgcgcgcgac gcgatcgacc tgatcgagcg cgcggtggaa aagcggcagg tgctgacgct
     6841 cgattacagc gacgaagcgg ggcgcggcag cgttcgcgac gttcgcccgc tcggcctgtg
     6901 gttctggggc aaggtgtgga cgctgatcgc gtggtgcgag atgcgcggtg atttccgcgc
     6961 cttccgcatc gaccgcatcg cttcggtggt tcttgccggg cgtgtcttca agccggagcg
     7021 cggcaagcag cttggcgatt tctaccgggc ggtggagcgc agcgaaggtt atggcgccgc
     7081 gccggaccgg gcgcggggaa gctgaccgca ggcccctccc gccgggaagg gcatgcccgt
     7141 attatccgtt gcgcaatgcg ggcgcaaagc cggtggcagg ttttgccgtg ccggccttat
     7201 tcgtcgtctt cgtcggtatc ctggtccttc gagcgcaagg ccgaaagctt ggcgaacacg
     7261 gcgttcgcat ccagttcctc gttctcgtcc ttcggctttt ccggcgcagg caccagccgt
     7321 tcggtaaggg ccgccggcag cagcgtatcg ccgacggggg cggcggcctc cttgccgcgc
     7381 gaggcgcggt tgacttccat gtcgaggtcg atctgcgagg tcaggccgag tgtcaccggg
     7441 tccatcggaa ccagattggc ggcattccag tgcttgcgat cacggatctg ctcgatggtc
     7501 gacttggtgg tgccgacgag ccgcgagatt tgcgcatcct tcaattcggg atggttgcgc
     7561 accagccaca aaatggcgtt cggccggtcc tgccgcttcg acagtggcgt gtaacgcggg
     7621 cctttgcgct tggattccgg cacgcgaacc ttcggttcgg acaatttcag gcgaagattg
     7681 ccgtcttttt cggcgcgcgc gatctcgtcg cgggtcaact ggccggtcat gatcggatcg
     7741 agacccttga tgccctgcgc cgcctcgccg tcggcgatag cctttacttc caggtgatgc
     7801 agtccgcaga actgtgcaat ctgctcgaag gaaagcgcgg tgttatcgac cagccagacg
     7861 gcggtcgcct tgggcatcaa cagcgtattg gccataaaaa gaatccttct cgttcgcccg
     7921 cgttcccgcg cgggcggtgg tttagagcca ccaatatggg aaattcgagg cctgtatagc
     7981 cgttcttcgg cccgttcgca acggttttgg gcggtttatg aaagtaggag tcccaccgca
     8041 tcggcatcgg caatccggga gcgcgccagc ctcaggcgtt ttgccggttc ggttcatgcg
     8101 gattttgcgg tgttgtcaaa acacgaccgc tattcgcaca tcacccgcac gcgcttgccg
     8161 gtccgggtgg cggcttcgca ggcgagcggc ggcgttgagc ccgatggcgc tgcggccggc
     8221 gctgcgcggc gcttcgcaga ccgcaccctc ggcctgcccg gcgattgtgg ctgcggcggg
     8281 accgggaagg tttcgggcgg cggcggcatg gcgatcgatg gatcggccgg cgcgcgcata
     8341 tagcctggat ggcgcaggtc gagcggaccg agatcaacgc gccgcatgtc cagcggcctt
     8401 gggataacga cggtaccatc gtccgttctg gagcaaccgc cggtcgccag aaggaggcag
     8461 aagacaaaag gaacagacct tcggggcatt ggtgacctct ccggcgccta tgtagcgcat
     8521 tgcaagccgc cggacaaagg catctcgcgc atggccatgc gctttaaggt caagttggaa
     8581 taaaattcca gccattaggg ttagctgatc cttaatgctt gtggggcaca ttgcgcgggc
     8641 gagccggtgg ttcgacctgc attgcctggg gtgagcgaga ttgtggtgat tcgagatgat
     8701 tccggcgtga agccgccggc gcgtcccgct aatatcagag cgtcggccag gaaggccgga
     8761 tcgccgccat tcctgcccgc cgaatcgctt cttgccctgg tcgaggcggg gtcggactgg
     8821 ctttgggaaa ccgatgccga tctgcgcttc tcgtgggttt cggagaatta ccagaaaatc
     8881 accggcatcg aagccgccca ggtgctcgga cggttccggt tcgactttct cagcccggca
     8941 agccagggca gcgaactggt cgcctcgcat ctggaggact tgcagcatca ccggcccttc
     9001 cgcgacttcg tctatgagct gaagggcagc cggcaggact gccgctggat ttccaccacg
     9061 ggctttccgc gcttcgatgc ggagggcaat ttcatcggct atcgcggcct cggccgcaat
     9121 gtcaccgaga tcgcggggac gctgggtaag cttgaaaagg cggcgccgca tgccgatcgc
     9181 gccgaattgc tggcttcggc cgaacgcaac cgccaattgc tgttcgatat cgagcggacg
     9241 atcgatgcgg tcgagatggg gatcgtcgtt gtcgacgcca atctcaacac ggagatcatc
     9301 aacaaggcct tctacaaaat atgggacatg accccgcaac agatcgcgct gggcagcccg
     9361 ttccggaaat tgatcgaatc cggccgatcc ggcgggttgg aggacattcg cgacgaggat
     9421 tgggaaagct atgtcgccag gcggctgaat gaggtgcggg caggcgacct tgcgccgcgc
     9481 gagttccggc ggaagaacgg ccgcaccacg atctatgcaa ttaccgcgct ttccggcggc
     9541 aagcggctgg tttcctacta tgatttgacg gagttgaaga agcaggaagc gcaggtggcg
     9601 gccgccaatg cgcgaaccga ggaggccttc tgcaatcttc gcatcatggt cgattccatc
     9661 cccatcggca tcatcgtcgt cgatgccgac atgcgggccg aaatcgtcaa ccgggcattt
     9721 tacgatttct ggagactaga cccggcgtcc atcggcatcg gggcaagttt tcgcgagctg
     9781 atggaagcga accgcaccat tgatccgcat ggcttcgatg cggaggcgtg gccccattac
     9841 atcgccaagc gcgaggctga aatcagggcg ggcgtggcgg aagtccgtca gctgacgcgc
     9901 ggcgacggcc gaacgctcat ttcctcgacc acgccgcttt ccggcggaaa gcgcctcatt
     9961 tcctatgtcg acgtaaccga catgaagaac cgcgaggcgg aacttgccga ggcgctggaa
    10021 aaggcgaagc tggccgaggc ggtcatcaac agcgtgccga acccgatgtt cgtcaaggac
    10081 gccgacctga acttcgtgat ggccaaccaa gccttcgccg atttcttcgg caccaccccc
    10141 gaccagatcc ttggccgaaa ggatgacgac ctcgtgccgc ctgaagagcg gggcatgttc
    10201 gaggaaagcg aaagacaggt tttggcgacc ggcgagttgt acgaaaccga agcgcaatat
    10261 gtgcgcgacg gcttccacgg cacgcgcgtc gttcgaaaga accgcttcga gacgaccagc
    10321 ggcaagattt atcttgccag ttcgcttttc gatgtcagcg aatccaagcg gcgcgagaaa
    10381 gaggccgagg aagcgcacag gcatttgacc aacgtgctgg aatccctgcc ggccggcgtc
    10441 gccgtctatg accgcgacga caaattcctg tttgccaatg aaaaattccg ggaaacgttg
    10501 ccggatttgc gcgccgcatg gacgccggga agcacgcttc gcgaggccct ggcgatcggc
    10561 catgcccatg gctatttccg cgacaatggc gacaaggcga tcgacggcct ttatgattgc
    10621 gatgtcgagg gctggctcga tgcgatggaa aaccattatc gccagcgcga cgcggtctac
    10681 gaacgggtca cgcccgaggg gcgctggtat caggtctaca acaagcgcac cgatgacggc
    10741 acgttcatcg gtgtgcgggt ggaaatcacc gacatcaagg agcgcgagcg cgcgctggag
    10801 gagagcctgc gtgaaaacga ggtgttccgc agcctgatcg acaatgtgcc ggtgtcgatc
    10861 tacgccaagc gcgcggacct gcggcaattc tacgtcaaca agggctggtg cgatctcacc
    10921 ggctacagca agcaggaagc gatcggcaag accgatatcg agatattcgg cgccgatggc
    10981 gaggcgttcg tcgaaagcga cctttccgtg ctgcggaccg gggaaacgca ggagatcgag
    11041 gaaacggtcg tatcggccga tggcagccgg cgccaccagt tcgcccgcaa aggcgcgatg
    11101 atcgcttccg acgggtcgct ttatctgatc ggctcgacaa ccgacataac ggagttgaag
    11161 cagcgcgagg ccgagttgca ggaggcgcgc cagcgcgcgg tgcttgccga ccgtgccaag
    11221 tcggaattcc ttgccaatat gagccatgaa atccgtacgc cgatgaatgg cgtgcttgga
    11281 atggccgaac tgcttgccaa gtccgacctc gatcccaagc agaagacctt taccgacatc
    11341 atcgtcaaat cgggcaatgc gctgctgacc atcatcaacg acattctcga cttctccaag
    11401 atcgatgccg gccagctggt gctcgacccc gccccgttca gccttgccga agccatcgag
    11461 gacgtcgcga cgctggtttc gacacgcgcc agggaaaagg acctggagct tatcgtacgg
    11521 atcgaacccg gcttcgacgg cctgttcgtc ggcgatgtcg gacgcgtgcg ccagatcgtc
    11581 accaatctgc tgggcaatgc ggtcaaattt accgacgagg gccatgtgct ggtcgacgtt
    11641 accggcgagc acgcgcccgg cgggacgcgg ctgcacatct cggtcaccga taccggtatc
    11701 ggcatccccg aagaaaagcg cgcgctggtg ttcgagaaat tcagccaggt cgacgcatcg
    11761 tcgacgcggc gccatgaagg caccggcctc ggccttgcca tcacctcgcg cctggtcgat
    11821 ctgatgggcg gcgagatcgg cgtggaaagt gccgaaggcc ggggatcgac cttctggttc
    11881 accattacgc ttgcgaccgc cgagcgcgcc ggcaagccgc gcatcacccc catcgacgtg
    11941 accggagcgc gcgtgctggt cgtcgacgac aatgccgtca accgctccat cctggccgaa
    12001 cagatggcgt cgtggaattt cgattcctgc gcggcggcaa gcggcgagga gggcctgaag
    12061 gtgctgatcg cggccgcggc ctatggcgtg ccggtcgatt gtatcgtcct cgattaccag
    12121 atgcccggca tgaccggtac ggagatggcg cgcatcgtgc gcaacaccga cagtttggcg
    12181 gatacgccaa tcgtcatgct gacctccgtc gaccagtcgc tggcgcaggc cagctaccgc
    12241 gaccttggca tcgatgccca cctcatcaaa ccggcgcggt cctcggcgct gctggaaaca
    12301 ctggtcacca ccatccagcg ccatcgcgcc aagacaatgc ctctcggcgc gacggcattc
    12361 gaggaaagtg ctgcttcatg cgcgccgaca ccggccatcg accggcaggc aggcgatgcc
    12421 gccggcaacg cgcttcggca gccgggcgcg atccggccgc gcagcgcccc ggtggcgggc
    12481 gaggggcgtc ggctcgatat actcgttgcc gaggataatg aggtgaacca gctggtattc
    12541 acccagatat tgggcgagac cggctacagt ttcgagatcg tcggcaatgg ccgcaaggcg
    12601 ctcgacgcct tcgcgcggct gaagccgctg atgatcctga tggacgtctc catgccggag
    12661 atgaacggcc ttgaggcgac ccaggaaatc cgccgcatcg agcaggatgc gggcacgcat
    12721 gtgccgatcg tcggcgtgac cgcgcatgcg ctcaagggcg accgcgagcg ctgcatcgaa
    12781 gcgggaatgg acgactacct ttccaagccc atcagcccca aggcgctgat ggaaaagctt
    12841 gaacgctggc tgggaacgga ccacgaagcc cagtgggccg gcaccgccgg ataagtcgtt
    12901 tcgcatcgcg gccaagactt tacccggcaa agcgtcatgc aagcgggaga gttgccgcga
    12961 cggctggttg gcgcgccgtc tgcgcaccgt tattgaagcg tcacgaacct gtcatgcaac
    13021 tgttataaat cacggctatg gcctcatcag gcgctaaaag aagcggcgcc gagagaccat
    13081 cttccgaaca ggagcaggcc ccatgagaca tttcatccgc tctgccgtcg ttgccgttgc
    13141 aatggccaca gcgtccacgc tcaccctttc gaccgcattc gccgccgaca tttccggcgc
    13201 aggttcgacc ttcatctatc cggtgttcgc caagtgggcg gatacctaca agaaggacac
    13261 cggcatcggt ctcaattacc agtcgatcgg ttccggtggc ggcatcaagc aggttgtcgc
    13321 caagacggtg accttcggcg cgaccgacaa gccgatgtcc gacgaagaac tggaaaagaa
    13381 cggcctcacg cagttcccga tggtgatggg cggcatcgtg ccgatcgtca acctcgaagg
    13441 cgtcaagccc ggcgaactca ttctcgacgg caagaccatc gccgacatct atatgggcgc
    13501 gatcaccacc tgggacgacg ccgcaatcaa ggcgctgaac cccgacctca cgctgccgtc
    13561 cacggcaatc gccgtcgtcc atcgctcgga cggctcgggc acgaccttca acttcaccga
    13621 ctacctcggc aagctctcgc cggactggaa ggaaaaggtc ggttcggaca ccgccgtcga
    13681 atggccgacg ggcgtcggcg ccaagggcag cgaaggcgtc gccaacaccg tcaagcagac
    13741 ggatggcggc atcggctacg tcgaatacgc ctacgcggcg cagaacgacc tgtcctacgc
    13801 caagatgatg aacgccgccg gcaaggtcgt ctcgccgtcg ctggaatcct tcggcgcggc
    13861 tgccgccaat gccgactgga agggcgccaa gaacttcaac gtcatcatca ccaacgaacc
    13921 cggtgacgag acctggccga tcgccgcttc gacctgggtg ctgatccaca aggctcccga
    13981 tgacgcggtt gctgccggcg aagcgctgaa attcttcgcc tgggcctatc aggacggtaa
    14041 gggcaccgcc aaggacctcg actatgtctc gatccccgac agcgtcgtcg acatgatcaa
    14101 ggcttcgtgg accgccgaca tcgctgccga cggcaagccg gtctacacgg tcggccagta
    14161 agctttgcca tcgatccgga ggggcgctga tgcggcgctc ctccttcccg caaaacgatc
    14221 cagacaatga ttgaggtcag cctgacatga gtgccgtcca ggaagccttg cctgccttca
    14281 agggctcgcg cgccgcgacc gtccgtcgct ttgcgctgac cgacacgatt ttccatgcgc
    14341 tgaccaaggc ttcggccatc ctcgtattgc tgctgttggg cggcgtcggc atttcccttg
    14401 tggccggatc atggccggcc ttgtcgacct tcggcttttc cttcctcgtc agcgaaagct
    14461 ggaacccggt caaggagaac ttcggggcgc tggcgccgat ctacggcacc gtggtcacgt
    14521 cggccatcgc catcctgatc gccgtgccca tcggcatcgg catcgccata ttcctgacgg
    14581 agctttgccc gcgcccgctg cgccgcccga tcggcatggc ggtggagctt ttggccggca
    14641 ttccgtccat catctacggc atctggggcc tgttcgtgct ggcgcccttt cttcagcgca
    14701 ccatccagcc tttcatcatc ggcgctttca agaacgttcc cggcctcaat ggcctgtttg
    14761 ccggcccgcc ctacggcatc ggccttttga cctcggcgct gatcctggcg atcatgatcc
    14821 tgccgttcat cacctcgatc accaaggatg tgttcgacac ggtgccgccg gtgctcaagg
    14881 agtccgccta cggcattggc tgcaccacat gggaagtaac gcggcgtgtg gtcattccct
    14941 acacccgcgt cggcatcatg ggcggcgtca tgctgggcct tggccgcgcg cttggcgaaa
    15001 ccatggcggt gaccttcgtg atcggcaacg cgcaccgcat cagcgagtcg ctatttgcgc
    15061 cggccacgac gatctcggcc accatcgcca atgagtttac tgaagcggtc ggcgacctct
    15121 acacctcctc gctggtggcg ctcggcctga tcctgttcgt catcaccttc ctcatccttg
    15181 cgctcgcacg ctacatgctg atgcgcatcg atgcccgcac gggagcctga caatggctgt
    15241 aacatcctcg ctccatagcc gccgcaagat ggcgaactcg atcatgatga cgctatgcgt
    15301 tgtcgcggcc gggatcgggc ttgcctggct ggcgctcatc ctcggcgcgc tggtctacaa
    15361 gggtgcatcc ggcctgtcgc tggccgtgtt cacccagatg acgccgccgc caggcgacgc
    15421 cggcggcctg ctcaacgcca ttttcggcag cgtggtgatg acggtcatcg cggttctcgt
    15481 cggcactccc atcggggtgc tggccggcac ctacatggcc gaatatggac gcttctcaag
    15541 gctgaccacg gtggtgcgct tcatcaacga cattctgctt tcggcgcctt ccatcgtcat
    15601 cggcctgttc gtctacgagc tgatggtgcg gccgatgggg catttctcgg cgatcgccgg
    15661 cgcggtggcg cttgccatcc tggttatccc ggtggtggtg cgcacgacag aagacatgct
    15721 caatctggtg ccgaacgcgt tgcgcgaggc gggcaccgcc atcggcgcgc cgcgctgggt
    15781 ggtcatcaag tcggtggcct accgcgccgc catgtcgggc atcgtcaccg gcatccttct
    15841 ttccatcgcc cgcatttcgg gcgagacggc gccgctcttg ttcacggcgc tcaacaatca
    15901 attctggagc ggcaacctca acgcgccgat ggccagcctg ccggtcacca tcttccagtt
    15961 cgctctcagc ccttacgagg aatggcagca actggcgtgg accggcgcac ttatcattac
    16021 cctgacggtt ctggtgttga gcatcatcgc cagaagccta accggacgca ggacatagag
    16081 atggcacaga tgttggctcc tgaaatgacc atgaccgatg acaccgccgg caaggccggc
    16141 aagaccaaga tcgaggtgaa gaacctcaat ttctactacg gccagtcgaa ggcgctgaag
    16201 gacattacgc tgtcgctgcc cgaacgcagc gtcaccgcct tcatcggccc gtccggctgc
    16261 ggaaagtcga cgctgctgcg cgtcttcaac cgcatctacg agctttaccc gaagcagcgc
    16321 gccgaagggc atgtgatgct cgacgggcag aacgtgctcg accgctcgca ggatttgaac
    16381 ctgcttcgca ccaagatcgg catggtgttc cagaagccga cgccgttccc catgtcgatc
    16441 tacgagaaca tcgcctttgg cgtgcgtctc tacgagaaga tcggcaagtc ggaaatggac
    16501 ggccgcgtcg agcaggcgct gaggcgcgcg gcgctgtgga gcgaggtcaa ggacaagctc
    16561 aacgccagcg gcctcagcct ttccggcggc cagcagcagc ggctgtgcat cgccaggacg
    16621 gttgcggtga agcccgaggt catcctgctc gacgaacccg cctccgcgct cgatccgctt
    16681 tcgacggcca agatcgagga gctgatcgac gaattgcagg ccgactacac catcgtcatc
    16741 gtgacgcaca acatgcagca ggcggcccgc gtctcgaagc agacggcctt catgtatctg
    16801 ggcgagctga tcgagttcga caagaccgag aagatattca cgtcgcccgg ccacaagcgc
    16861 acgcaagact acatcaccgg ccgtttcggc tgagccagtc attgccatta gcgatacgag
    16921 gacagtttca tgaccgaaca taccgtcgcc gccttcgatg acgatctcgg ccagatcggc
    16981 cgcctcatcc agggcatggc cgaccttgcc ggggcgatgg tcagcggggc cacgcatgcg
    17041 ctgcttaccg ccgacaacgc acaggcgcag cgcatcgttt ccgacgacgc attgatggat
    17101 gcgcgccagc gcgaactgga cgagcgcgcc atcacgctga tcgccaggcg ccagccgatg
    17161 gccgacgatc tacgctcggt ggtcggcgcc atccggatgg ccggcgacct cgaacgcatc
    17221 ggcgacctgg ccaagaacat cgccaagcgc gtcgccgcgg tcggtgaaag cgccgtcccg
    17281 cgcgagcttt cgcattcgat cgacggcatg gcgcagttgg tgctggaaca ggtgcgcggc
    17341 gtcatggcgc attacagcgc gcaggatgcc gccggcctta tcacgctgag ggacgacgac
    17401 gagcgcatcg acgtcaaata cacctccgtc ttccgcgaat tgctgaccta tatgatggaa
    17461 gacccgcgca acatcacggc ctgcacgcat ctgttgtttt gcgccaagaa cctggagcgc
    17521 atcggcgatc acgtcaccaa tatcgcggag aacgcctatt atgtgatgac cggccagcaa
    17581 ttgccggccc atcgcccgaa atcggatgaa atggtaatgt cggccccggc tgcctgacct
    17641 gaaggtcctt gcgccggttg gatgcgcagg ggatgcttca atagattgat cgagcaccgc
    17701 gcggaaaccg acagccgagt ttcggcgcgc tgctccagac aggaagacac gatcgatgat
    17761 tgcaccgcgc atcatggtgg tggaggacga agagccgcta ggcgtgctct tgcgttataa
    17821 tctcgaatcc gaaggctatc atgtcgaggt ggtgacgcgc ggcgacgagg cggaaatccg
    17881 cctccaggaa aacgttcccg accttctggt gcttgattgg atggtgccgg ccgtatcggg
    17941 catcgagctt tgccgccgct tgcggatgcg ctcggaaacg gagcgcctgc cgatcatcat
    18001 gctgacggcg cgcggcgagg aaagcgaccg ggtgaggggg ctttcgaccg gtgccgacga
    18061 ctatctggtc aagccgttct cgacgccgga attcatggcg cgggtcaagg cgctgctgcg
    18121 gcgcgccaag ccggaagtgc tttcttcggt gctgaaggtc ggcgacatcg tgctcgaccg
    18181 tgaaacccac cgcgtctacc gcgacaaaag cgagatcagg cttgggccga ccgaatttcg
    18241 gctgctcgaa ttcatgatgc gctctcccgg ccgcgtattc tcgcgcgggc agttgctgga
    18301 caatgtctgg ggcgagacaa tctatgtcga cgagcgcacc gtcgacgtgc atgtcgggcg
    18361 gctgcgcaag gcggtcaatg acggccatgc gcaagacgtc atccgcacca tccgcggcgc
    18421 cggctacgcc atcaaggaag aatgatttcg ttcgccggtc aggccgggcg tacaccgggc
    18481 gcggtgctcc atggcatcgg ccatggcaag cgcagagccg ccgcatcggg cggcgaaccg
    18541 gccgatttgc cggacggcct caaggctcca gttcgacatc ccagtagaga tagtccatcc
    18601 agctttcatg cagatggttg ggcgggaaca gccggccatt attgtgcaaa tcgtgcacgg
    18661 tcggctgata gggcttttgc ctgggaaaca tcttggcctg cgccggcatc atgccgccct
    18721 tggtcaagtt acagggcgaa caggcggcca ccacgttttc ccatgtcgtg gcgccgccgc
    18781 agcggcgcgg gatgacgtga tcgaaggtca aatcctcggg cgtgccgcaa tattggcact
    18841 ggaaacggtc gcgtaaaaac acgttgaagc gggtgaaggc tggatggcgc gacggcttca
    18901 cataggcctt caggctgacg acgctgggca atttcatcga gaatgtcggc gaggagacgg
    18961 catgctcata ttcggcgacg atgttcacgc ggccgagaaa cacggccttg atggcgtcct
    19021 gccatgacca gagcgacaac gggtaatagc tgagcggacg gtaatccgca ttcagcacca
    19081 gtgctggaag cccatccgcg gatacggcaa ccgtcacgtt tcgacctcct tcgcttccga
    19141 cccgaatgtc gccgatactg taagcccgac atgacaggga tgtgaagcgg attgtcagcc
    19201 tgccgacacc gcaaaactgc gcggtttcaa tggtttttcg acatttcagg ccggaggcgc
    19261 ggcctcgcgg cccttcaatt ggcggtagta ggcccaaaaa aggcgcgccg cgaccccgcg
    19321 ccagggcgac catgattcgg ctatggcgat cagcgtcttt tccggcgggc gcggcgcaat
    19381 gcccagcgca tggccgacag cggtttgcag cgccacgtcg cgcgcgggga aaatatcggg
    19441 gtggccggcg gcaaacagaa gatagacctg cgccgtccaa ggcccgatgc cgggaaccgc
    19501 cagcagcagc ttcatcgcct cgtcggcatc cagccggcat aggtgataaa ggtcgagccc
    19561 gtccgcgacc gcctgggcca cggccagcaa gccgcgctgc ttcggccgcg acaggccggc
    19621 ctggcgaaac acggcgtcgt cggcggcaag gatagcctgc ggcgtcagcg gatcgacgat
    19681 ctttaccagc cggccgaaga tggcgtcggc gctggcgcgc gaaacctgct gcgagacgac
    19741 gatcgaggcg aggctcctga agccgggttc cgacagcctg agcggcacct cgccgaccat
    19801 ggcgcgcacc gccaaaaggc gcggatcgag cacgccgagg gcgtcgaggc cgcgttctat
    19861 gtcgtctagc gttgcaatat gttgcatggt tgccttgttg gcgcgccggg atcaaagtag
    19921 caatcgacaa aagcgcagca caacccgatt tgcggccgaa gcatgagcct gccgacattc
    19981 cgtttcgcgc ctagtcccaa tggcgaactg catcttggcc atgcctattc ggcgctgctc
    20041 aaccagaagc tggcgaaggc cgccggcggt cggcttttgc tgcgcatcga ggacatcgac
    20101 acggtgcgtt gcacgcccga gttcgaggcg gccatcttca acgagctgaa ctggctcggc
    20161 gtcgggtggg aaaagccggt gcgccggcaa tcgcagcatt tcgcggaata tgcggaaatc
    20221 ctgaaccggc tgatcggcga ggggctggtc tatccggcct tcatgagccg cggcgaaata
    20281 cgcgcgttca tcaccgaccg ccagaagcgc ggacttgatt ggccgcgcga ccccgatggc
    20341 gtgccgctct atcccggcgc ggacagggct ctgcccgatg cgcagcgcaa gcggcgcatc
    20401 gccgaaggcg aaccctatgc gtggcggctg gataccgcag ccgccatcgc ccgcatcggc
    20461 cggccgctgt catggatgga acatgccgat gaaaatctcg ataccgccag tcccgtcgag
    20521 gcccgcccgc aggactgggg cgacgtcatc ctcgcccggc gcgacgtgcc gacaagctat
    20581 cacctggcgg tcgtcgcgga cgatgctctg caaggcgtga gccatgtcgt gcgtgggcgg
    20641 gatctgtttc tcgccacctc gctacagcgg ctcctccagg aattgctcgg cctgccgcaa
    20701 ccggtctatt gccaccatcg gctgatcctg gggtcagacg gccgcaagct gtccaagagc
    20761 ctcgccggca ccagccttgc cgagctgcgc aggggcggcg ccacagcgca cgacattcga
    20821 aacatgcccg gcctgtgacc ggcatggaaa gccttccatc aattcccgtt attctatagt
    20881 tcagcggttt cgttttgcgc gccgccgcga acggcactac aggcagacgc gcgtttcctc
    20941 cagtaatcga tggctcagtt gcacagtctc aagcggttca ttccggccat attcgtcgtc
    21001 ttgtgggcaa ccggcttcat cggcgcgcgc tatgccatgc cctgggtgga gccgtttacg
    21061 ttcctttcca tgcgcttcgt catcgccacg atcttgctgg cggcaatcgc cgtggcgctt
    21121 gggctgaagc gggcgacgcg caaggaagcg ctcaacgcgg caatcgccgg cgcgctgatg
    21181 cacggcgtct atctgggcgg cgtgttctgg gcgatccatc gcggcatgcc ggccggattg
    21241 tcggcgctga tcgtcgggtt acagccgctg gtaaccgccg tgatggcggg caaactgctc
    21301 ggcgaaagga tcctgccgcg ccactgggtc ggcctggcga cgggcttcgc cggtgttgtc
    21361 atcgtgctgt tgccgaagct tggcgaactg ggcagcggca tcgacgcgca gaccgtttcc
    21421 gcctgcatct tcgccgtttt cggcataacg ctcggctcga tctggcaaaa gcgctttgcg
    21481 ctgagcggca acctgttcgc gaccacggtg tggcaatatg tgggtggcgc gctggtgacg
    21541 gtgccggccg cgcttttgtt cgaaacccgc agcgtcgtct tcaacggcga actcgccttt
    21601 gcgctggcgt ggctggtgct ggtgctctcc atcggcgcgg tgttcctgct gatggtgatg
    21661 atccgtgaag gcgaaatgtc caaggtcgcc tcggtgttct acctggtgcc ggcggtgacg
    21721 gcggtgatgg cgtgggcgct gtttggcgaa acgctcgacc tcatccagat cgccggcatg
    21781 gcgatcacca ccttcggcgt cgcgctggcg acggctcagt cgggaacccg cgcccgcgct
    21841 tccagatagc gtatgatggc ggcgttgaat tcaccgccga ggatgaagat ggccgagacg
    21901 atatacagga acaccaccgc gatcatgatc gaggcaaggc cggcataggt cgtcacatag
    21961 gatgagaaat ggtcgagata ggtggcgaag atggtcgatc cgatcagcca gcctatcagc
    22021 gtgaagacga tgccgggaat gagcgacagg aagcgccgct tgccgatcgg cagccagtaa
    22081 tgcacggcga aaagcccgcc cacgataacc gtcgaggcga tgatgtaacg ccatagcgtg
    22141 atggtgccca tatagggctt gatccattcg aactcggcct cggccagccg cgccaggatc
    22201 ggcgccagca ccagaagcac gctgatgatg aggaagccga tggtggcgat gatgacgaag
    22261 aacaggctct gcgcgcgccg gtagatgatg ccgcgcgttt ccaccacccg gtaggcgcgg
    22321 ttgagcgagg tgcgcagcgc ctcgatgccg ttcgaggcga agtaggcggc cagcagcacg
    22381 ccataggtca acaggtcggt gcgctgcacg gtcagcacgt tgagcacctc gcgcgcgatg
    22441 ggcttggcga tctgttccgg ccaggtgtcg aacaccaggt ggacggccgt gtcggcgaag
    22501 gcctgcgcgc cgaggaacga cgccaacgtg gtggcgaaga tgagaaacgg aaacagcgcc
    22561 atcagcgccg tgatggccag atgactggac atggcccagc cgtcatcgtt gttgaaatga
    22621 ccgagcgcgt cgtaaacgac gcgtttgaag gcaacgacgt tccgtagcaa gcacgtttcc
    22681 tgcgattgtt tcccggtcgc gaatatggga agggaatgcg gcaaatggca agcctcgaag
    22741 gagcaacggc tttccggtga aagagttgcg cacaatcatc gtcaccggcg cttcgtcagg
    22801 cgtcggcgct tattgcgccc gagccttgaa ggcggatggc tggcgcgttt ttgcaacggc
    22861 gcgcaaggaa gaggacatcg ccgcgctgga aacggcgggc atcgaggcgt tcccgctcga
    22921 ctaccgcgac ccggcctcga tcgaagcatt ggtggcggcg gtcctggagc gaacgggcgg
    22981 tagcctcgac gccctctaca acaatggcgc ctacgcccag gctggcgcgg tcgaggatgt
    23041 gccggtcgaa ctattgcgcc aacagtttga ggcgaacttc ttcggctggc acgacctgac
    23101 ccgccgcgtc atgccggcga tgcgcaggca ggggcatggc cgcatcgtca actgttcatc
    23161 cattctcggg ctcgtgcccc tgccttatcg tggcgcttac tgcgcctcga aatatgcgct
    23221 ggaagggctg atgctttcca tgcgccagga actggccggc agcggcatcc atgtttcgct
    23281 gatcgaaccg ggacccatcg cctcagacct gcacgataac ggccttccct ggttcaggga
    23341 ctccatcgat cttgagaact cggtccaccg tgaagcctat cgccggcaga tggatgagct
    23401 atggtccggt ccgccgcgcg caaaggccgg gccggtggtg gtctacacag ccttgaagca
    23461 tgcacttctg tcgcggcgtc cgcgtccgca ttatgtggta acgatgcctg caagaatcgg
    23521 cgtggtgctg aagcgcatct tgcctgcttc gttattttac cggcttcttg ccagaagcac
    23581 ctgagcgaac tggaattgtc atgggcaccg ttttcaatat cctcgccgtc atcgtcatgg
    23641 tcgccgtggt ggccgtcctg ttgcgcggcc ttttcaacat gatgcgcggc ggcgacggca
    23701 tgacctccaa caaattgatg caggcgcgcg tcctgctgca attcgtggca gtcgtgctga
    23761 tcatgctggc ggtctatttc acccgtaagt aagcggcgga actggacgga gaaagccggt
    23821 ggtcaagctc aacaagattt atacccgcac cggcgacgat ggcacgacag ggcttggcac
    23881 cggcgagcgc cggctgaaat ccgacctgcg ggtggaagcc tatggcacgg tcgatgaggc
    23941 caatgcctgc atcggcatgg cccgcatcca taccgctaaa acctatccgg caatcgacgc
    24001 catgcttggc cgcatccaga acgatctgtt cgacctcggc gcagaccttg ccgtgcccga
    24061 cagcggcgaa ccgctcgatt atgagccgct gcgcattacc gccgcccagg tggagcggct
    24121 gg
//
LOCUS       DASYCS010000444         9615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_422086,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000444 DASYCS010000000
VERSION     DASYCS010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 9615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9615
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422086"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VGM46_13640"
     CDS             complement(<1..240)
                     /locus_tag="VGM46_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY4193684.1"
                     /translation="MGHLETKRILVAGTGPAGLIAALAIAKAGFAVTLVGPETVADDR
                     RTTALMNPALEMLDSLGVLAQLAEVSAPLKIMRIVD"
     gene            546..1358
                     /locus_tag="VGM46_13645"
     CDS             546..1358
                     /locus_tag="VGM46_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015317886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylcholine/phosphatidylserine synthase"
                     /protein_id="HEY4193685.1"
                     /translation="MIVRKNTQGRKIASRIPRPKKKVTWPQARAFSVHLLTASGSFLA
                     FLSLVAASEERWTAMFWWLGLALFVDGIDGPIARKLDVKEILPTWSGELLDNIIDYVT
                     YVLIPAFALYQFGLMGEKLSFLSAAIIVVSSAIYYADTGMKTKENFFKGFPVVWNMVV
                     FTLFVIGPGEWTSFAVVVIAGLLTFVPINFLHPVRVKRLRPINLTITLLWCAFGALAL
                     AESALAEFYSQIGVLAKNVSIFTKIGIAGTGLYLFCIGGVMQFFPKLGAKKA"
     gene            1563..3140
                     /locus_tag="VGM46_13650"
     CDS             1563..3140
                     /locus_tag="VGM46_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019858083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4193686.1"
                     /translation="MGGTVSAQHDAANLLEVRGLTKIFGTLTACDHIDLDIAKGEIHA
                     LLGENGAGKSTLVKMLFGSLEPNSGEIFWNGEPVRIPRPGVAKKLGIGMVFQHFSLFE
                     ALTAAENIALSLDDGSPIGSIAAKARALSYSYGLPLDPDSLVGDLSVGERQRIEIIRC
                     LLQTPQLIILDEPTSVLTPQEADKLFETLERLRSEGKSILYISHRLEEVKRICDRATV
                     LRHGKVVGHCNPRDETASSLARMMVGNEVHTVVRQPAEGIEQASSLLEIRNLTRKPAT
                     PFSIPLRNINLNVRAGEVIGIAGVAGNGQGEFFEAVSGEVRQQDHASVRIRGKDAGDL
                     GITGRRLLGAAFVPEERLGHGAAPGMKLSENLLLSRYATDRKVFVGTGGLVQSGAVYG
                     AAQRIIQEMDVRKSAPDPEAAALSGGNLQKFIVGRELDRTPSVMVVNQPTWGVDAGAA
                     AHIRQALIELARQGSAVLVISQDLDELFEISDAIAVMHNGSLSTPLPIAEATFEKIGL
                     LMGGAEPGHLEHALETA"
     gene            3137..4234
                     /locus_tag="VGM46_13655"
     CDS             3137..4234
                     /locus_tag="VGM46_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006333605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4193687.1"
                     /translation="MMRLELVKRPQRSVLFSILSPFIAFVLTVIAGAIMFALLGVNPI
                     NGLYTYFISPVTQVWQLHELAIKAAPLILIAVGLSVCYRANIWNIGAEGQFILGGIVG
                     SIIPVMYPELEGPFVLPLMLLLGMVGGAAYAAIPAFLKARFNTNEILTSLMLVYVAQL
                     LLDWMVRGPWRDPKGFNFPQTVLFSDSATLPQIFPDSGRANWGFVFALVAAVLVWILM
                     SRTLKGFEVRVLGSSPRAGRFAGFGFNKMVFFTFMLSGALAGLAGISEVSGAIGQLQP
                     SISPGYGFAAIIVAFLGRLNPLGIVAAGLVLGLTYLGGEAAQTALGISDKVARVFQGM
                     LLFFVLGCDTLIHYRIRLVGLAAAPKAEEAH"
     gene            4234..5208
                     /locus_tag="VGM46_13660"
     CDS             4234..5208
                     /locus_tag="VGM46_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4193688.1"
                     /translation="MDITVNILLTIATAATPLLIAAIGELVVERSGVLNLGVEGMMIM
                     GAVGGFGAAYYTGSPWIGMMAAIALGAIFSLLFAFMTLTLATNQVATGLSLTILGLGL
                     SGMMGTSFVGQPGERLPNLDIPGLSSIPVIGRLLFGQDPIFYISIALTAAVMWFLFRT
                     RSGLTLRSIGDSHSSAHALGIKVIRYRYLAVIFGGACAGLAGGHLSLVYTPQWVENMS
                     AGRGWIALALVVFASWRPWRVLAGAYIFGAIWIGQLHVQAIDLNDFVGAPWAVYKLVE
                     IIKSFPPQLLSSLPYLATVVVLVLISRNKRLTMMNTPASLGQPFVPDR"
     gene            5259..6326
                     /locus_tag="VGM46_13665"
     CDS             5259..6326
                     /locus_tag="VGM46_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004681884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY4193689.1"
                     /translation="MKKLLIALMTTTAALSMAAGAQAQEKLKACWVYVGPIGDFGYSY
                     QHDQGRLEVQKELGDKVETAYLENVAEGPDAERAFERLAREGCKIIFGTSFGFMDSEV
                     KVAKRFPDIKFEHATGYKTSDNLGIYNARFYEGRYVMGQIAAKESKAGVAGYIVSFPI
                     PEVVMGINAFMLGAHSINPEFKAKIVWVNSWFDPGKEADAAKALFDQGADIIVQHTDS
                     TAALQVAEERKLKGFGQSSDMIKFAPHAQLTANTDNWGPYYVERVKAVLDGTWKADNV
                     WLGIKDDAVHLSDFTNMSDETKAMAEATEKKISGGWNPFTGPIDKQDGTPWLKDGEVA
                     DDATLLGMNFYVKGVDDKLPQ"
     gene            complement(6403..7056)
                     /locus_tag="VGM46_13670"
     CDS             complement(6403..7056)
                     /locus_tag="VGM46_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531644.1"
                     /note="Members of this highly hydrophobic protein
                     family,regularly are found preceded by the yybP-ykoY
                     manganese riboswitch (see RF00080). A metal cation
                     transport function is proposed; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjbE family putative metal transport protein"
                     /protein_id="HEY4193690.1"
                     /translation="MEIFTAAGLTALLQVIAIDLVLAGDNAIVIGLAAAGLPAAQRKK
                     AILVGVIAATVLRIAFAAITVKLLAVVGLLLAGGILLLWVCWKMWRELRTSHADEQEA
                     TEALSNSDGKASAGSGKPRKTLAQAAMQIVIADVSMSLDNVLAVAGAARDHFSVLIIG
                     LILSIGLMGLAASFIAKLLHRHRWIAYIGLLIILYVALDMIYRGVLEVWPHMNAIAG"
     gene            complement(7471..7860)
                     /locus_tag="VGM46_13675"
     CDS             complement(7471..7860)
                     /locus_tag="VGM46_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008875792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF930 domain-containing protein"
                     /protein_id="HEY4193691.1"
                     /translation="MRNLCLATLALAVIGLPAHAMDARLRSGLLKLDPQTRLEQRCDA
                     EVLDRISHDDRKYNADRVVAYAFATPEMSDDGIRSPGAAFRSKGKWYRLKFKCRTAPD
                     HMEVLQFRYRIGDQIPESDWSKYNLYP"
     gene            complement(8033..8122)
                     /locus_tag="VGM46_13680"
     tRNA            complement(8033..8122)
                     /locus_tag="VGM46_13680"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8086..8088),aa:Ser,seq:tga)
     gene            complement(8316..9278)
                     /locus_tag="VGM46_13685"
     CDS             complement(8316..9278)
                     /locus_tag="VGM46_13685"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEY4193692.1"
                     /translation="MRRCRPVPFTQKLLMSLLTDCVWNLAILRLPMRRRRRASVLADP
                     CPICRKMPRNNIRLIQFERSGMPAYLGRSRISEMTKSAKRKLVLIRHGKGPCDDRVSV
                     FGAEHGYELEYRHPFDGDALGLPEDDVAGAVIFGGRFEAYETQKYPFLKQEARWIEAC
                     IDQGIPLLGICQGAQQIAHTLGGRVGPPPSNAHEFGYYPLLPTEKGRTTFPGPMRVTQ
                     AHFHTFDIPAGAVHLASSEDYPNQAFRYGQRTYAFQFHPEVTIEGFRRWQMSLSGLFG
                     KPGAQTREEQDRLIYDSDRVQAAWFYAFLSKLFGTAKGSDTQSD"
     gene            9353..>9615
                     /locus_tag="VGM46_13690"
     CDS             9353..>9615
                     /locus_tag="VGM46_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008875777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEY4193693.1"
                     /translation="MQTSARLRLRRASAFVLLAASAGLLAACATPQPKVAAGRNHSKE
                     YFAESKYGVKASPRVAFGRFGIKRGGGRDQIGKPYQVAGKWYYP"
BASE COUNT         1891 a         2909 c         2824 g         1991 t
ORIGIN      
        1 atcgacgatg cgcatgattt tcaacggagc cgaaacctcg gccaattgtg caagcacgcc
       61 cagactgtcc agcatttcca gtgcagggtt catcaacgcc gtggtgcggc ggtcgtcggc
      121 caccgtctcc ggcccgacca acgttaccgc gaacccggcc ttggcaatgg ccagtgcggc
      181 gatcagcccc gccggtcccg ttccagcgac caatatccgc tttgtttcca gatgacccag
      241 gatcggcttc cgtttttgtc aatggacagg cccgaaagcc tgcgaagtct tcttataaag
      301 cgttctttca cccatgttca acgcggcctt ttcgaccatt cgaagcacaa gcgcctcaca
      361 atcatggggg aggcttgatc gcccccttgt tccggcggcg gcgctgctgt ttagtcttgg
      421 ccggatgggg aaggcgatcc ggcttgtctg cgggcaaagc ttcggcgcat ggccgcggcg
      481 ataacccgga atgtcacgcc gaaaatttcc ccggcgcttt ttgaggcatc aggtttggac
      541 tggcgatgat tgtgcgcaag aacacacaag gcagaaaaat cgccagcagg attccgcgac
      601 cgaaaaagaa agtcacctgg ccgcaggccc gtgctttctc ggttcatctg ctaaccgcgt
      661 ccggttcttt tctcgctttt ctttcgctgg tcgccgccag tgaagagcgc tggacggcga
      721 tgttctggtg gctggggctg gcgctgtttg tcgatggaat tgacgggccg atcgcgcgca
      781 agctcgacgt caaggaaata ctgccgacgt ggtcgggtga actgctcgat aacatcatcg
      841 actatgttac ctacgtgctg attccagcct tcgcgctcta ccagttcggc ctgatgggcg
      901 agaaattgtc gttcctgtcg gcagccatca tcgtggtttc cagcgcgatc tactatgccg
      961 acaccggcat gaaaaccaag gagaacttct tcaagggttt tccggtggtg tggaacatgg
     1021 tggtgttcac tttgtttgtc atcgggcctg gcgaatggac ttcgtttgcg gtggtggtga
     1081 tcgcaggcct gctcactttc gtgccgatca atttcctgca cccggtgcgg gtcaagaggc
     1141 tgaggccgat caacctgacc atcaccttgc tatggtgcgc gttcggcgca ctggcgctgg
     1201 ctgaatcggc gcttgccgaa ttctacagcc agatcggggt gctcgccaaa aacgtcagca
     1261 tcttcaccaa gataggcata gcgggcacgg gtctttatct tttctgcatc ggcggggtca
     1321 tgcagttttt cccgaaattg ggcgctaaaa aagcctgaag tttcaaattt tgcgtcaaag
     1381 accatcattt ccgccgggta tgacaggaac gcattccacc ggggcgcaag caatcgtgac
     1441 cgtccgcacc gctcgacttc ctgctgaatt tcttgaagct ctttcaagac tggtggcgct
     1501 ttgcgttatg ggcaaccaat gcctacgatg cttgcgggaa cacagaacat gtctggtaac
     1561 agatgggagg cactgtgagt gcacaacatg atgccgccaa ccttttggag gttcgcggcc
     1621 tgaccaagat attcggcacg cttaccgcgt gcgaccacat tgatctcgac atcgcaaaag
     1681 gtgagattca cgcgcttctc ggcgagaatg gcgcgggcaa atcgacgctc gtaaaaatgc
     1741 tgttcggttc gctcgaaccc aattcgggcg agattttctg gaacggcgaa ccggtacgta
     1801 ttcccaggcc gggcgtggcg aagaagttgg gtatcggcat ggtattccag catttttcgc
     1861 tcttcgaggc gctgacggct gccgagaaca ttgcgctttc actcgatgac ggttcgccca
     1921 tcggcagcat cgcggccaag gcgagggctc tttcctacag ctatggcctg ccgctcgatc
     1981 ccgactcgct ggttggcgac ctgtcggttg gcgagcgcca gcgcatcgag atcatccgct
     2041 gcctgctgca aacgccgcag ctcataatcc tcgacgagcc gacttcggtt ctgaccccgc
     2101 aggaggccga caagcttttc gagacgctgg agcgcctgcg tagcgagggc aaatcgatcc
     2161 tctatatttc gcaccggctg gaggaggtaa agcgcatatg cgaccgcgcc accgtcctgc
     2221 gccacggcaa ggttgtgggt cactgcaatc cgcgcgacga gacggcatct tctcttgccc
     2281 gcatgatggt gggcaatgaa gtgcacaccg tcgtgcgcca gccggccgag ggcatcgaac
     2341 aggcgtcgtc gctgctggag atacgcaatc ttacccgtaa accggcgacc cccttctcga
     2401 tccccttgcg caacatcaat ctgaacgtcc gcgccggcga agtgatcggc atcgccggtg
     2461 tcgccggcaa tggacagggc gagttcttcg aggcggtttc gggggaggtg cgccagcagg
     2521 atcatgcctc cgtccgcatc cggggcaagg atgccggcga cctcggcatt accggccgcc
     2581 gattgctggg tgcggctttc gtgcccgaag aacggctggg ccacggggcg gcgcccggca
     2641 tgaagctttc ggaaaacctg ctgctctcgc gttatgcgac cgaccgcaag gtttttgtcg
     2701 gaacgggcgg cctggtccag tccggcgccg tctacggggc ggcccagcgc atcatccagg
     2761 aaatggacgt gcgtaaaagt gcgccggacc cggaagccgc ggctctttca ggcggtaatc
     2821 tgcaaaaatt catcgtcggc cgcgaactcg accgcacgcc ttccgtcatg gtggtgaacc
     2881 agccgacatg gggcgtcgac gcgggcgccg ccgcccacat ccgccaggcg ttgatcgaat
     2941 tggcgcgtca aggctcggcg gtgctggtca tcagccagga cctcgatgag ttgttcgaga
     3001 tttcagatgc catcgcggtg atgcacaacg gctcgctgtc gacgccgttg ccgattgccg
     3061 aagcaacctt cgaaaagatc ggcttgctga tgggcggcgc ggaacccggc catctcgaac
     3121 acgcgctgga gaccgcatga tgcgcctcga actcgtcaaa cgcccgcagc gctcggtgct
     3181 gttttccata ctgtcgccgt tcatcgcctt cgtgctgacg gtgattgccg gcgccatcat
     3241 gttcgcgctg cttggcgtca acccgatcaa cgggctctac acctacttca tctcgccggt
     3301 cacgcaggtc tggcagttgc atgaactggc gatcaaggcc gcacccctca tcctcatcgc
     3361 ggtcggcctt tccgtctgct atcgcgccaa tatctggaat atcggtgctg aaggccagtt
     3421 catcctcggc ggcatcgtcg gttcgatcat cccggtcatg tatcccgaac ttgaaggccc
     3481 gttcgtcttg ccgctgatgt tgctgctcgg catggtcggc ggcgctgcct atgcggcgat
     3541 ccccgccttc ctcaaggccc gcttcaacac caacgagata ttgaccagcc tgatgctggt
     3601 ctatgtggct caacttttgc tcgactggat ggtgcgcgga ccgtggcgcg atccgaaagg
     3661 cttcaatttt ccgcagacgg tgctgttcag cgactccgcc acgctgccgc agattttccc
     3721 ggattccggc cgcgccaatt ggggctttgt cttcgccctg gtggcggcgg ttctcgtctg
     3781 gatattgatg agccgaacgc tgaagggttt cgaggtccga gtgcttggat ccagcccaag
     3841 ggcagggcgc tttgccggct ttggcttcaa caagatggtg ttcttcacct tcatgctttc
     3901 cggcgcattg gcaggtcttg ccggcatttc ggaagtatct ggagccatcg gccagctaca
     3961 gccctcgatt tcgcccggat acggctttgc cgccatcatc gtggcgtttc tgggccgcct
     4021 caatccgctc ggcatcgtcg ccgccggcct ggtgctgggc ctgacctatc tcggcggcga
     4081 agcagcgcag accgcgcttg gcatctcgga caaggtcgcg cgcgtcttcc agggcatgct
     4141 tttattcttc gtgctcggct gcgacacgct catccactac cgcatacgac tggtcggcct
     4201 ggcggcagcg ccaaaagccg aggaggcaca ctgatggaca tcaccgtcaa catattgctg
     4261 acgatcgcca ccgccgccac cccgctgttg atcgccgcga tcggcgaact ggtggtcgag
     4321 cgctccggcg tgctcaacct cggcgtcgag ggcatgatga tcatgggcgc ggtcggcggc
     4381 ttcggcgcgg cctattacac cggttcgccg tggatcggca tgatggcggc catcgcactg
     4441 ggtgcgatat tttcgctgct gtttgctttc atgacgctga ctctcgccac taaccaggtt
     4501 gccaccggcc tttcgctgac catcctcggc ctcggccttt cgggaatgat gggaacaagc
     4561 ttcgtcggcc agcccggcga aagactgccc aatctcgaca ttcccggtct gtcatccatt
     4621 cccgtcattg gccgcttgct gtttggccag gacccgatct tctacatttc gatcgcgctg
     4681 acggccgccg tcatgtggtt cctgtttcgc acccgcagcg gccttacctt gcgctcgatc
     4741 ggcgacagtc acagctccgc gcacgcgctt ggcatcaagg tcatccgcta ccgctatctg
     4801 gcggtcatct tcggcggcgc ctgcgccggc ctggccggcg ggcatctgtc cctggtctat
     4861 acgccgcaat gggtggaaaa catgagcgcg ggtcgcggct ggatcgccct ggcactggtc
     4921 gtcttcgctt cctggcggcc atggcgggtt ttggccggcg cctacatctt cggcgcgatc
     4981 tggattggcc agctccatgt ccaggcaatc gacctgaacg atttcgtcgg cgcgccctgg
     5041 gcggtctaca aactggtgga aatcatcaaa tcgtttccgc cgcagcttct ttcttcattg
     5101 ccctatctgg caactgtggt ggttctcgtt ctaatctcgc ggaacaaacg cctgacgatg
     5161 atgaacacgc ctgcttcact tgggcagccc ttcgtcccgg accgctgata acgatagatg
     5221 aatcaaaagc gtagagctta atacggagag gtgacttgat gaaaaaactg ctcattgccc
     5281 tgatgacgac gacagcggcg ctatccatgg ccgccggcgc gcaggcacag gaaaaactca
     5341 aggcctgctg ggtctatgtc ggcccgatcg gcgatttcgg ctattcctac caacacgacc
     5401 agggccgcct cgaagtgcag aaggaactcg gcgacaaggt cgaaaccgcc tatctggaaa
     5461 atgtcgcgga aggtcccgat gcagagcgcg ccttcgagcg tctggcgcgc gaaggctgca
     5521 agatcatctt cggcacctcc ttcggcttca tggattccga agtgaaggtt gccaagcgct
     5581 tccccgacat caagtttgaa catgccaccg gttacaagac gagcgacaat ctcggcatct
     5641 acaatgcccg cttctatgaa ggccgctacg tgatgggcca gatcgcggca aaggaatcca
     5701 aggccggcgt cgccggctac atcgtttcct tcccgattcc cgaagtggtg atgggcatca
     5761 acgccttcat gctcggcgcg cactcgatca atcccgagtt caaagctaag atcgtctggg
     5821 tcaactcctg gttcgatcca ggcaaggaag ccgacgccgc caaggcgctg ttcgaccagg
     5881 gcgcggacat catcgtccag cacacggact cgaccgccgc ccttcaggtc gccgaagagc
     5941 gcaagctgaa aggcttcggc cagtcttccg acatgatcaa gttcgccccc catgcccagc
     6001 tcaccgcgaa caccgacaat tgggggcctt actatgtcga gcgcgtcaag gccgtgctcg
     6061 acggcacctg gaaggcggac aatgtctggc ttggcatcaa ggacgacgcc gtccacctgt
     6121 ccgacttcac caatatgtcc gacgagacca aggcgatggc cgaggcgacg gaaaagaaga
     6181 tttccggtgg ttggaacccc ttcaccgggc ctatcgacaa gcaggacggc acgccgtggc
     6241 taaaggacgg cgaggttgcc gatgacgcga cgctgctggg catgaatttc tacgtcaagg
     6301 gcgtcgacga caagctgccg caataatacg cggttctccg tgcaaaataa aaagggcgcc
     6361 tcacggcgcc ctttttatgg gagaaagacc tgttgccgga aatcatcccg caattgcgtt
     6421 catatgcggc cacacctcaa gaacgccgcg atagatcatg tcgagcgcga catagaggat
     6481 gatcaacagg ccgatatagg caatccaccg atggcgatgc agcagcttcg cgatgaagct
     6541 cgcagccagt cccatcagac cgatcgacaa gatcaggccg atgatcagca ccgagaaatg
     6601 atcgcgcgcc gcgcccgcca ccgccaacac gttgtcgagc gacattgaaa cgtctgcgat
     6661 gacgatctgc atggcggcct gggcaagcgt cttgcgcggt ttgccgctgc cggcagatgc
     6721 ttttccgtcc gaattggaca gcgcctccgt cgcctcctgt tcgtcggcat gggaggtgcg
     6781 cagttcgcgc cacattttcc agcataccca taaaagcagg atgccgccgg caagcagcag
     6841 gccaacgacg gccagcaact tgaccgttat ggccgcaaaa gcgatgcgca gcaccgtcgc
     6901 cgcgatgaca ccgacgagga tggccttctt gcgctgcgcg gcaggcaaac ctgccgcagc
     6961 aagaccgatg acgatggcgt tgtcgcccgc aagaaccaga tcgatggcga tcacctgtaa
     7021 cagggcggta aggcctgcgg ccgtgaaaat ctccatcgtc aaaacgccct ttctgtcaaa
     7081 tgaaccgctg ccggcaaccc agccgaatat gtggatttca tgggcaaaac ctggagatca
     7141 gcctccaacg ctacccacca aggccatgcc gcaaattctt gtgggccaaa gcccataggt
     7201 gcttttggca caaggaatgc cgcgatggcc agagattttc cagggataat tccggcatcg
     7261 gaatttgcgg cctgggcgag gtgccgcccc actgcaaaag acacggcgat gagatcaagc
     7321 agggcgtgta taaggtcgaa cgtgccagcg gctcgcggca ttgaagagtt cggccgcccg
     7381 atacgcccgg cgcgccctat atttcctcag cttcgcttgg atcacctgcg caatgccgtg
     7441 gatgcgctga atgtcgatcc agcccaaacc ttacggatag agattgtact tggaccagtc
     7501 gctttcggga atctggtcgc cgatccggta gcggaactgc aagacttcca tgtggtcagg
     7561 cgcggtccgg catttgaatt tcagccgata ccattttccc ttgctgcgaa aggcagcgcc
     7621 agggctgcgg atgccgtcgt cgctcatctc cggcgtggca aaggcatagg cgacaacgcg
     7681 gtcggcattg tatttgcggt catcatggga aatgcggtcg agcacctcag cgtcgcaacg
     7741 ctgctcaagc cgggtctggg ggtcgagttt caacaggccc gaccgcagcc gggcatccat
     7801 ggcgtgagcc ggaaggccaa taaccgcaag ggcgagggtc gcgagacaaa gattcctcat
     7861 ggcgcgaaac caccatagca tgtcgcgaaa atcaaccccg caccaaagct atccaggcgc
     7921 ccgacacgca aactttaccg ctacaggccg agcagctagt gccggcacaa ttgtgtgaac
     7981 ggaatgggct ggcgcggaat gtgcaatgaa tacaattcga tatgtgccag cttggcggag
     8041 ggagtgggat tcgaacccac ggtgagcttg cacccacgcc ggttttcaag accggtgcct
     8101 taaaccgctc ggccatccct ccgtcgtgat ttttcaatca cttaagatat gcgcttaatg
     8161 ccaaatcata ctgtgtggcc ttgaaagcga tacagcgcat tctcacgctt ctacaggaaa
     8221 atcctgtagg gcgcgctcca gcgctgtgtc aaccgccgcc tcgatgttct tggcgctttg
     8281 aacgcagccg ccgagccgct gcactgggtt ccgtttcagt cgctctgcgt atcggagcct
     8341 tttgccgtgc caaacagctt gctaaggaac gcgtaaaacc acgcagcctg gacccgatcg
     8401 ctatcgtaaa tcagccggtc ctgttcctcg cgcgtctgcg cgccgggctt gccgaacagg
     8461 cccgacagcg acatctgcca ccggcggaaa ccctcgatcg tcacttccgg atgaaactgg
     8521 aaagcgtagg tgcgctggcc gtagcggaac gcctggttcg gatagtcttc gctcgacgca
     8581 aggtgaaccg caccggctgg aatgtcgaag gtgtggaaat gcgcctgcgt cacccgcatc
     8641 ggccctggaa aggtcgtgcg gcctttttcg gtcggcagta gtggataata tccgaattcg
     8701 tgggcgttcg aaggcggcgg acccactctg cctccaagcg tgtgggcgat ctgctgcgcg
     8761 ccctggcaga tgccaagcag cggtattcct tgatcgatgc aggcttcgat ccagcgcgct
     8821 tcctgcttca ggaatggata tttttgcgtt tcatacgctt cgaacctccc accgaagata
     8881 acggcgccgg ctacgtcgtc ttcaggaaga ccaagcgcgt cgccatcaaa tgggtggcgg
     8941 tattcaagct catagccatg ctcggcgccg aataccgata ccctgtcgtc gcacgggcct
     9001 ttgccgtggc gaatgagaac cagtttccgt tttgcagatt tggtcatttc agaaatccgg
     9061 ctcctgccaa ggtaagctgg catgcccgaa cgctcgaatt gaatcaatcg aatgttgttg
     9121 cgaggcatct tccggcaaat agggcagggg tcggcaagca ccgaagcacg ccggcgcctg
     9181 cgcatcggca gcctcaatat ggccaggttc cagacacaat cggttaatag gctcataaga
     9241 agtttctggg taaaaggaac agggcggcag cggcgcaagc atcttcgata tggcaagctt
     9301 gcgagcgatg ttacggtttc catagcattg gacggcaggg gacaacagcg acatgcagac
     9361 ttcggcgcgc ctgcgtcttc gccgcgcttc ggccttcgtt ttgctggccg cttccgcagg
     9421 ccttttggcc gcatgcgcca cgccgcagcc gaaggtggcc gccggcagga accactccaa
     9481 ggaatatttc gcggaatcca aatatggcgt gaaggctagc ccgcgcgtgg cgttcggacg
     9541 cttcggcatc aagcggggcg ggggcaggga ccagatcggc aagccctatc aggtcgccgg
     9601 caagtggtat taccc
//
LOCUS       DASYCS010000445         9585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4047013,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000445 DASYCS010000000
VERSION     DASYCS010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 9585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9585
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4047013"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..117
                     /locus_tag="VGM46_13695"
     CDS             <1..117
                     /locus_tag="VGM46_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEY4193694.1"
                     /translation="IQLGDTFWRVSGPDLPAGARVRVVSATETDLELTVEPV"
     gene            complement(114..1094)
                     /locus_tag="VGM46_13700"
     CDS             complement(114..1094)
                     /locus_tag="VGM46_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEY4193695.1"
                     /translation="MKQPDRQASIASALRTVKTERAGVDALAAALENGLAEPLAEAVE
                     ILAGIEGRVIVTGVGKSGHIGSKLAATLASTGTRAFFVHPSEANHGDLGMIAHDDAII
                     AMSWSGETAELKGIIAYSRRFSIPLIAVTAGQKSALARQADVLLALPRAPEACPHGLA
                     PTTSALLQLVIGDALAVALLEARGFTPDHFRTFHPGGQLGANLTQIREIMHVGDRLPM
                     VASGTGMREAILELSNKGFGCVAIVDGDGALAGIITDGDIRRHIGSDLLSMTVDQVMT
                     RGPKTVEPDTLVAKALQVLNASSITAVMVEENRRPIGLVHLHDLLRIGAA"
     gene            1261..3108
                     /locus_tag="VGM46_13705"
     CDS             1261..3108
                     /locus_tag="VGM46_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY4193696.1"
                     /translation="MLRLQPRKASHGTGESVRALLLATSVLALTLALTLPVAVKAQEM
                     GLRGEVSESTIQRDQLNGAGKANATRAGPKSTVPSAGGQNGTPVPAYDPASVGAIPDP
                     TGDAEDDAGQDAGTIDGMTASRPDNSAFSDDPVPAAPAPQWKPTDDDAQEEATPLKPG
                     EKPAQSRTAKAKAKAKGKASPAEGDTGLANPDQADQDDPEQQAANLRAGTLDSAEKKP
                     LDRGAERAETIEGLRKKREDDPFAAVGIRAGSFILRPTLEEGVTATSNADSSFNGSSA
                     VLSETTLRLNAISDWKENSASSDGYLTYRKTLSGDDIEDVRGRAEGTLNIDIGHDLRA
                     IAKFGYEAAPESASSPVVIEDTISQPLRQTIDGSLGIRKDVGKARFGLTGAVKHDIYG
                     DAELSSGGTLSQKDRDSTLYTATLRGGYEISPALTPFVEIETGRRLYDESIDTSGYDR
                     SANRLGARAGLELDMGEKLAGEFSAGWLREAIDDDRLAPISGATVNADLKWSPERGTT
                     VGLSGATIVEGTTNPGESGSLLYSSRLSVERQIRDNLTGNAALGVDWRDYVGSNGHDL
                     TLSAEASLTYWLNRYVGLTTRARTEKLTSNLPGREETTNSIFVGLKLQR"
     gene            complement(3151..4056)
                     /gene="galU"
                     /locus_tag="VGM46_13710"
     CDS             complement(3151..4056)
                     /gene="galU"
                     /locus_tag="VGM46_13710"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006199459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEY4193697.1"
                     /translation="MKKVRKAVFPVAGLGTRFLPATKAIPKEMLTVVDRPVIQYVVDE
                     AREAGIEHFIFVTGRNKAVIEDHFDIQFELNHTLAQRGKHDQMAKLERLQPKAGQTSF
                     TRQQEPLGLGHAVWCARELVGDEPFALLLPDMITLPDHGCLKDMVDLYDHAGGSIIAV
                     EECDPSEAHKYGIVGKGEDVHTGFRINGMVEKPKPGTAPSNLYINGRYILQPQIFDIL
                     ARQERGAGNEIQLTDGMLKLQAQHPFHGYHYRGRTFDCGSPEGFVEANVAFALARSDM
                     NANMGGVIRTLLDEMNPEQRKAVAV"
     gene            4248..5492
                     /locus_tag="VGM46_13715"
     CDS             4248..5492
                     /locus_tag="VGM46_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006199460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEY4193698.1"
                     /translation="MKKEEKVPVRNAARRFARTMSIIGLSLAVVMPSTSAFADAGFRQ
                     WVAGFRATAVQHGVSGSIYDRAFRGINDPDPVVLEKARYQPEFTAPAWDYFDNRVHDQ
                     SIAVGQEMARKWKPWLDRIERRYGVDRHILLAIWSMESNYGEILKRPDVMRSVVRSLA
                     TLGYADKRRSKFARTQLIAALKILQTGDIDESHLSGSWAGAMGHTQFIPTSYQSYAVD
                     MDGNGKRDIWNSVPDALATAANLLHKNGWQPGKTWGYEVVLPAGRKFPPGSMSLSKWA
                     ALGVTRANGKPFRNSSDSATLKVPGGREGPAFLMIKNFSVIKRYNNADKYAIAVGLLA
                     DEIAGYGGLVQDWNRPFTKLSFEERQELQQRLSEHGYYEGKFDGKIGESSKAAILAYQ
                     AKAGLTPDGHPSMEVLKWLRRK"
     gene            5512..6675
                     /locus_tag="VGM46_13720"
     CDS             5512..6675
                     /locus_tag="VGM46_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HEY4193699.1"
                     /translation="MAFRRLATLILALIVIGAGMAVMRTGAAMAQDQGWSLRDLLFPR
                     RQERAQSLERFPPPPKAKKQRAKRPPVVVAPRTVEKAPDARVILVIGDFMASGLADGL
                     EAAFEDNRAIRIVDRSNGSSGFVRNDHYDWTKEVGPIVEAEKPAAVIAMIGSNDRQQM
                     QVGETRETMGSAAWTTEYKARTQALGKALAAAKARFLWVGMPAFKSAKATSDMLAFND
                     IYRAAAQDNGGEFIDVWDGFVDETGAFVTNGPDINGQPVRLRGDDGINVSKAGRRKLA
                     FYTEKPLMKILGLASPAEPGAASAQPAGEPVKPPEAATAPPAAIDRTVPMLLSDPALD
                     GGTELLGGSAESRAGQPSREAAKTAAVPAGRADNFAWPAGKAVPNDKTTAVNP"
     gene            complement(6724..8178)
                     /locus_tag="VGM46_13725"
     CDS             complement(6724..8178)
                     /locus_tag="VGM46_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004677801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HEY4193700.1"
                     /translation="MGKVTGFLEIDRQVHKYQPASDRIRHFREFTLPMSDKEVEKQAA
                     RCMDCGVPYCHGPTGCPVHNQIPDWNDLVYSGDWDNAIRNLHSTNNFPEFTGRVCPAP
                     CEEACTLNLEDIPVAIKTIEQALADKAYETGHIRPYPPEQKSGKRVAIVGSGPAGMAA
                     AQQLGRAGHEVHVYERESRPGGLMRYGIPDFKIEKHFIDRRIEQMRGEGVTFFCGVNV
                     GVDKPVAELLAEYEAVLYCGGSETPRAAGIPGDDLEGVHDAMPYLVQQNRRVGGEDIQ
                     SVAWSSPPVHASGLHVVVVGGGDTASDCVGTAFRQGAVRVTQLDIRPQPPEKEDKLAV
                     WPYWATKMRTSSSQAEGAEREFQVATLEFIGEEGQLTGVRCCEVDDRRKPIAGSEFVI
                     RADLAFIAIGFAGPMSEGVVSELSGQMKTATDNRRSNNVVANDRDYRTSVDRLYAAGD
                     VRRGQSLVVWAIREGRQAARAIDEALMGSSVLPS"
     gene            complement(8272..>9585)
                     /locus_tag="VGM46_13730"
     CDS             complement(8272..>9585)
                     /locus_tag="VGM46_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HEY4193701.1"
                     /translation="GLRTGRDVVIGALLGADEFGFATAPLIAAGCIMMRKCHLNTCPV
                     GVATQDPVLRKRFKGTPEHVINFFFYVAEEVRALLAEMGFTHLDQIIGDTDLLEKQGL
                     IEHWKARGLDFSRMFFKLDVPSEQTHWTERQKHPIADVLDRKLIELAAPALDAKQPVH
                     IEVPVRNVDRSVGAMLSGEVAKRFRHKGLRDDTINVKLTGTAGQSFGAFLTRGVSFHL
                     EGAANDYVGKGLSGGRLVIRPPQDSKIVAAESIIVGNTVLYGATEGEAYFCGVAGERF
                     AVRNSGVVTVVEGVGDHGCEYMTGGIVVVIGQTGRNFAAGMSGGVAYVLDEAGDFAAR
                     CNMAMVELEPVPEEDDLMEKLLHHGGDLDHKGRVDVTGDMTSHDEERLYQLISNHVHY
                     TGSARGKEILDDWVNFRPKFRKIMPVEYRRALIEMERSRMGVAAE"
BASE COUNT         1784 a         3166 c         2948 g         1687 t
ORIGIN      
        1 atccagcttg gcgatacgtt ctggcgcgta tcggggccgg acctgccggc gggcgcgcgt
       61 gtgcgcgtcg tctcggccac ggagaccgat ctggaactga ccgtcgagcc tgtctaggcc
      121 gcgccgatgc gcaacaggtc atgcaggtga acgaggccta tgggccggcg gttttcttcc
      181 accatcaccg cggtgatcga cgaggcattg agcacttgca gcgcctttgc caccagcgtg
      241 tccggctcga cggtcttggg gcctctggtc atcacctgat cgacggtcat cgatagaaga
      301 tcgctgccga tgtgacggcg aatgtcgcca tcggtgatga tgccggcaag cgcgccgtcg
      361 ccgtcgacaa tcgccacgca gccgaaaccc ttgttcgaca gttccaggat ggcttcgcgc
      421 atgcccgtgc cggacgccac catcggcagc ctgtcgccaa catgcatgat ctcgcggatc
      481 tgcgtcaggt tggcgccaag ctggccaccg ggatggaaag tgcggaaatg gtccggcgtg
      541 aagccccgcg cttcgagcag cgccaccgcc agtgcatcgc cgatcaccag ttgcagcagc
      601 gccgatgtcg tcggcgccag cccgtgcgga caggcttcgg gcgcgcgcgg cagcgccagg
      661 agaacgtcgg cctggcgtgc cagcgccgat ttttgtcccg ccgtcaccgc aatcagcgga
      721 atggaaaagc gccgcgaata ggcgatgatg cccttcagct ccgctgtctc gccggaccat
      781 gacatggcga tgatggcgtc gtcatgcgcg atcatgccga gatcgccgtg attggcttcc
      841 gaggggtgga cgaagaacgc acgcgtgccg gtcgaggcga gcgtcgccgc aagcttggag
      901 ccgatatggc cgcttttgcc gacgccggtc acgatgacgc ggccctcgat tccggccagt
      961 atttccacgg cttcggccaa tggctcggcc agaccgtttt ccagagcggc cgccagcgcg
     1021 tcgacgcccg cccgctccgt cttaacagtc ctcagcgccg atgcgatcga tgcctggcgg
     1081 tccggctgct tcattcgaga taccccagaa agttcgatag tcatgcgatt accgcctggc
     1141 cgtgccgctg tccaacggaa ttggcttttg gccggtggcg ctggcgcctt ctcaaccctc
     1201 cgttaaccat ccccatttac ggttcggtaa gtttggcgcg cctgcgccgc cgaggtcaaa
     1261 atgctgcgtc tccaacccag aaaggcaagc catggcacgg gcgaatccgt tcgcgcgctg
     1321 ctattggcga cgagcgttct ggccctgact ctggcgttga ccctgcccgt tgccgtcaag
     1381 gcgcaggaaa tgggcttgcg tggcgaggtt tccgaatcga ccatccagcg ggatcaactg
     1441 aacggggcag gcaaggccaa cgccacccga gccgggccga aatcgacggt gccttccgcc
     1501 ggcggccaga atggaacgcc cgttcccgcc tatgaccccg caagtgtcgg cgcgataccc
     1561 gaccccacag gcgatgccga ggacgatgcc ggacaagacg caggcacgat tgacggcatg
     1621 accgcatcca ggccggacaa cagcgcattc tcggacgatc cggttcctgc cgcgccagcg
     1681 ccgcaatgga aaccgaccga cgacgacgcg caagaagaag ctacgccgct caagccgggt
     1741 gaaaaaccgg cccaaagccg gacggcgaag gcgaaggcca aagccaaggg caaggccagc
     1801 cccgccgaag gggataccgg cttggccaat cccgatcagg ccgaccagga cgacccggag
     1861 cagcaggcgg cgaacctgcg cgcgggcaca ctggattctg ccgagaagaa accactcgac
     1921 cgaggcgccg aacgcgccga gacaatcgaa ggactgcgca agaagcgcga ggacgacccg
     1981 tttgccgccg tcggcatcag ggcgggttcg ttcatattga ggccgacgct cgaagaaggc
     2041 gtcaccgcca cctcgaacgc cgattcgagc ttcaacggct cgtcggccgt gctttcggaa
     2101 acgacgctgc gcctcaacgc catttcggac tggaaggaga attcggccag ttccgacggt
     2161 tatctcacct atcgcaagac cttgtccggc gacgacatcg aagacgtgcg tggccgcgcg
     2221 gaaggcaccc tcaacatcga cataggccac gacctgcgcg ccatcgccaa gtttggctac
     2281 gaggctgcgc cggaatccgc ctcttcgccc gtcgtcatcg aggatacgat ttcgcagccg
     2341 ctgcgccaga ccatcgatgg cagcctcggc atccgcaagg atgtcggcaa ggcccgcttc
     2401 ggcctgacag gcgcggtaaa gcacgacatc tatggcgatg cggaattgtc gtccggcggc
     2461 accctttcgc agaaggaccg cgattccacg ctctacaccg cgacgctgcg cggcggttac
     2521 gagatttcgc cggctttgac gcccttcgtc gaaatcgaga ccggtcgcag gctttacgac
     2581 gagagcatcg acaccagcgg ctacgaccgt tccgcgaacc ggcttggcgc gcgcgccggc
     2641 ctcgaactgg acatgggcga gaaactggcc ggcgagttct cggccggctg gctgcgcgaa
     2701 gccatcgatg acgaccgcct ggcccctatt tccggcgcaa ccgtcaacgc cgatctcaaa
     2761 tggtctcccg aacgcggcac cacggtgggc ctttcgggcg ccacgatcgt cgaaggcacc
     2821 accaaccccg gcgaaagcgg ttcgctgctc tattcaagcc ggctttcggt ggagcggcaa
     2881 attcgcgaca acctcaccgg caacgcggcg cttggcgtcg attggcgcga ctatgtcggc
     2941 tccaacggac atgacctgac gctgagcgcg gaagcgagcc tgacttactg gctgaaccgc
     3001 tatgtcggcc tgaccacgcg cgcgcgaacg gagaagctga ccagcaacct gcccggccgc
     3061 gaggaaacca ccaacagcat ctttgtcggc ctcaagcttc agcgctagcc gccggctgcg
     3121 gcctggaacc cggccctcgc ggcgctttcg ctagaccgcg accgccttgc gctgttccgg
     3181 gttcatctcg tccagcagcg tgcgaataac gccgcccata ttggcgttca tgtcgcttcg
     3241 cgccagggcg aaagcgacat tggcctccac gaacccctcc ggcgaaccgc aatcgaaggt
     3301 gcggccccgg taatggtagc cgtgaaacgg gtgctgcgcc tgcaacttca gcatgccgtc
     3361 ggttagctgg atctcgttgc ccgcgccacg ctcctgcctg gcgaggatat cgaatatctg
     3421 cggttgcagg atgtagcggc cattgatgta gagattggac ggcgcggttc cgggcttcgg
     3481 cttttccacc atgccattga tgcggaagcc ggtatgaacg tcctcgccct tgccgacgat
     3541 gccgtattta tgcgcctcgg aaggatcgca ttcctccacc gcgatgatgc tgccgccggc
     3601 atggtcgtaa agatcgacca tatccttcag gcagccatgg tcgggcagcg tgatcatgtc
     3661 tggcaaaagc agcgcgaacg gctcgtcgcc caccagttcg cgcgcgcacc acacggcgtg
     3721 gccgaggccc agcggctcct gctgccgggt aaagctggtc tggccggcct tcggctgcaa
     3781 tcgctcaagc ttcgccatct ggtcgtgctt gccgcgctgc gccagcgtat ggttcagttc
     3841 gaactggatg tcgaaatgat cctcgatgac cgccttgttg cggcccgtaa cgaagatgaa
     3901 atgctcgatt cccgcctcgc gggcttcatc aacgacgtac tggatgaccg gccggtcgac
     3961 gaccgtcagc atttccttcg gtattgcctt ggtagcggga agaaaacgtg tgccgaggcc
     4021 ggcaacagga aatacggcct tgcgaacttt tttcatacta tcgatcatcc ccgctatagg
     4081 gcctttcggc aattgtactg acggatgcta tcacagccgg attgaagatt cctcaatgcc
     4141 gcccgcggca tccgatcaca ataagtatct ttaggccttt tcgttccccg gtgaccctat
     4201 ggtaaactaa ttgataactt gcttgcgcca tgactgatct tttctggatg aagaaggagg
     4261 aaaaggtgcc cgtccgcaac gcagccagac gcttcgcacg cacgatgtcg atcatcggcc
     4321 tctcgcttgc cgtggtcatg ccttccacct cggcctttgc cgatgccggt tttcgccagt
     4381 gggtagccgg cttccgtgcc accgccgtac agcatggcgt ttcgggttcg atctacgatc
     4441 gcgcctttcg cggcatcaac gatcccgatc ccgtcgttct ggaaaaggcg cgctaccagc
     4501 ccgaattcac cgcgcctgcc tgggactatt tcgacaatcg cgtgcacgac cagtcgattg
     4561 ccgtcggcca ggaaatggcg cgcaaatgga agccgtggct cgaccgcatc gaacggcgct
     4621 atggcgtgga ccgccacatc cttctcgcca tctggtcgat ggaatcgaat tacggcgaaa
     4681 tcctcaagcg cccggacgtc atgcgcagcg tcgtgcgttc gctggccacg ctcggctatg
     4741 ccgacaagcg gcgctccaaa ttcgcccgca cccagctgat cgcagcgctc aagatcctcc
     4801 agaccggcga cattgacgaa agtcatctca gcggctcctg ggccggcgcc atgggccata
     4861 cccagttcat cccgaccagc taccagtcct acgccgtcga tatggacggc aacggcaagc
     4921 gcgacatctg gaattcggtt cccgatgcgc tggcgaccgc cgccaacctt ctccacaaga
     4981 atggctggca gcccggcaag acctggggct acgaggtcgt gctgcccgcc ggccgcaaat
     5041 tcccgcccgg ctccatgtcg ctgtcgaaat gggcggcgct cggcgtcacg cgggccaatg
     5101 gcaagccctt caggaacagc tccgacagcg ccacgctgaa ggtgccgggc ggccgcgaag
     5161 gaccggcctt cctgatgata aagaatttct cggtcatcaa gcgctataac aacgccgata
     5221 aatacgccat cgccgtcggc ctccttgccg acgagatcgc gggctatggc gggctggtgc
     5281 aggactggaa caggcctttc accaagctga gcttcgagga acggcaggaa cttcagcagc
     5341 gactgtccga acatggctat tacgaaggca agttcgacgg caagatcggc gaaagctcga
     5401 aagccgccat cctggcgtat caggccaagg cgggcctgac gccggatggc caccccagca
     5461 tggaagtgct caaatggttg cgcaggaagt agtcaccgga gacagatagg catggctttc
     5521 cgccgcctgg cgaccctcat cttggcgttg atcgtcatcg gggccgggat ggcggtcatg
     5581 cgaaccggcg cggccatggc gcaggatcag ggctggtcgt tgcgcgacct tctgtttccg
     5641 cgccggcagg aacgcgcgca atccctggag cggtttccgc cgccgcccaa ggcgaagaag
     5701 cagcgcgcga agcgcccacc ggttgtcgtc gcaccccgga cggtggaaaa ggcgcccgac
     5761 gcgcgcgtca tcctggtgat cggcgacttc atggcgtccg ggctggccga cgggcttgag
     5821 gccgccttcg aagacaatcg cgccattcgc attgtcgatc gcagcaatgg ctcctccggc
     5881 ttcgtgcgca acgaccacta cgactggacg aaagaggtcg gcccgatcgt cgaggccgaa
     5941 aagcccgccg ccgtcatcgc catgatcggc tccaacgacc gccagcagat gcaggtgggt
     6001 gaaacgcgcg agacgatggg ctcggcggcc tggacgacgg aatacaaggc ccgcacccaa
     6061 gcgctgggca aggcgcttgc cgccgccaag gcccgcttcc tctgggtagg catgccggca
     6121 ttcaaatccg ccaaggccac gtccgacatg ctggccttca acgatatcta ccgggcggcc
     6181 gcgcaagaca atggcggtga attcatcgat gtatgggatg gtttcgtcga cgagaccggg
     6241 gcattcgtca ccaatggacc ggacataaac ggccagccgg tgcgcctgcg cggcgacgac
     6301 ggcatcaatg tcagcaaggc tggcaggcgc aagcttgcct tctacaccga aaagccgctg
     6361 atgaagatcc tgggccttgc ctcgccggct gaaccgggtg cggcctcggc ccagccggcc
     6421 ggtgaaccgg tcaagccgcc ggaagcagcc acagccccgc ccgccgccat cgaccgtacc
     6481 gtgccgatgc tgctgagcga cccggcgctg gacggcggca ccgaacttct cggcggatcg
     6541 gcggagagcc gggccggcca accgtcacgc gaagcagcca aaaccgccgc cgttcccgca
     6601 ggccgcgccg acaactttgc atggcccgcc ggcaaggctg ttcccaacga caagacgaca
     6661 gccgtcaacc cttagcgcgt cgatccaggc aggccatatg gccttgcctg tcaggtcgcc
     6721 tcgtcagctc ggcaggacgc tcgatcccat caaggcttcg tcgatggcac gggcggcttg
     6781 ccggccctca cggatggccc acaccaccag agactggcca cggcgcacgt cgcccgccgc
     6841 ataaagccgg tccacgctgg tacgataatc acggtcattc gccacgacat tgttcgagcg
     6901 gcggttatcg gttgccgtct tcatctggcc gctcagttcc gaaaccaccc cctcgctcat
     6961 cggcccggca aagccaatgg cgatgaaggc cagatcggcg cggatgacga attcggaacc
     7021 ggcaatcggc ttgcgcctgt catcgacctc gcagcagcga acgccggtca actgcccttc
     7081 ctcgccgatg aattccagtg tcgccacctg gaattcgcgc tcggcgcctt ccgcctggct
     7141 ggaagaggtg cgcatcttgg tcgcccaata gggccagacc gcaagcttgt cttccttttc
     7201 cggcggttgc ggccggatgt cgagctgcgt cacacgcacc gcgccctggc gaaaagcggt
     7261 gccgacgcaa tcggacgccg tatcgccgcc gccgacgacg acgacatgca ggccgctggc
     7321 gtgaaccggt ggcgatgacc aggcgacgga ctggatgtcc tcgccgccga cgcgccggtt
     7381 ctgctgcacc agatagggca tcgcatcgtg gacgccctca agatcgtcgc ccggaatgcc
     7441 ggctgcgcgc ggcgtttcgg aaccgccgca gtaaagcacc gcttcgtatt cggccagcag
     7501 ttcggcaacg ggcttgtcga cgccgacatt gacgccgcag aagaaggtta cgccctcgcc
     7561 gcgcatctgt tcgatgcggc ggtcgatgaa gtgcttctcg atcttgaagt cgggaatgcc
     7621 atagcgcatc agcccgccgg gccggctttc gcgctcatag acatgcacct cgtggcctgc
     7681 gcggccaagc tgctgcgctg ccgccatgcc ggccgggccg gagccgacga tggcgacacg
     7741 cttgccgctt ttctgctctg gcggataggg ccggatatgg cccgtctcat aggccttgtc
     7801 ggccagtgcc tgttcgatag tcttgatggc gaccggaatg tcctcaaggt tgagcgtgca
     7861 ggcttcctcg cagggcgccg ggcagacgcg tcccgtaaac tcgggaaaat tattcgtcga
     7921 atgcaggttg cggatcgcgt tgtcccaatc gccactgtag acgagatcgt tccagtcggg
     7981 gatctggtta tgcaccgggc agccggtcgg cccatggcaa tagggcacgc cgcaatccat
     8041 gcagcgcgcc gcctgtttct caacctcctt gtccgacatc ggtagcgtga attcgcggaa
     8101 atgccggatg cggtcggaag ccggctggta cttgtgcacc tgccggtcga tttcaaggaa
     8161 acccgttacc ttgcccatgt cagactttca tttgtaactc ggcgcgcttt cgcggcctgt
     8221 ttgcaatccc gatggaatgc cggcccgttg gccggcatca tgaagctttt gctattccgc
     8281 cgccacgccc atgcgcgagc gttccatttc gatcagcgcg cggcgatatt cgacaggcat
     8341 gatcttgcgg aatttcggcc ggaaattcac ccagtcgtcg aggatttcct tgccccgcgc
     8401 cgagccagta taatgcacat ggttggagat aagctggtaa agccgctcct cgtcatggct
     8461 ggtcatgtcg ccggtaacgt cgacgcggcc cttgtgatcg aggtcgccgc catgatgcag
     8521 cagcttctcc atcaaatcgt cttcctccgg caccggctcc agctcgacca tcgccatgtt
     8581 gcagcgcgcg gcgaaatcgc ccgcctcgtc cagcacatag gcgacgccgc ccgacatgcc
     8641 ggcggcgaag ttgcgtccgg tctggccgat gacgacgacg atgccgccgg tcatatattc
     8701 gcagccatgg tcaccgacgc cctcgacgac ggtaacgacg cccgaattgc gcaccgcgaa
     8761 acgctcgccg gcgacgccgc agaaataggc ctcgccctcg gtcgcgccgt aaagcacggt
     8821 gttgccgacg atgatggatt ccgccgccac gatcttgctg tcttgcggcg ggcggatgac
     8881 gaggcggccg ccggaaaggc ccttgccgac atagtcgttg gccgcccctt ccaggtggaa
     8941 cgaaacgccg cgcgtcagga acgccccgaa agactgcccg gccgtcccgg tcagcttcac
     9001 gttgatcgta tcgtcgcgca ggcccttgtg cctgaagcgc ttggcaacct cgcccgaaag
     9061 catcgcgccg accgaacggt cgacgttgcg caccggcact tcgatatgca ccggctgctt
     9121 tgcgtcgagc gcgggtgcgg ccagttcgat cagcttgcgg tcgagaacat cggcgatcgg
     9181 gtgcttctgc cgttcggtcc aatgcgtctg ctcggacggc acgtcgagct tgaagaacat
     9241 cctggaaaaa tcgaggcccc gcgccttcca atgctcgata aggccctgct tctccagaag
     9301 gtcggtatcg ccgatgatct ggtcgagatg ggtgaagccc atttcagcca gcagcgcgcg
     9361 cacttcctcc gccacgtaga agaagaagtt gatgacatgc tcgggcgtgc ccttgaagcg
     9421 cttgcgcagc accgggtcct gcgtggcgac gcccaccggg caggtgttca ggtggcactt
     9481 gcgcatcatg atacagccgg ccgcgatcag cggcgcggtg gcaaagccga actcgtcggc
     9541 gcccagcagc gcgccgatca ccacgtcgcg gcccgtgcgc aggcc
//
LOCUS       DASYCS010000446         2578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1874064,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000446 DASYCS010000000
VERSION     DASYCS010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1874064"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VGM46_13735"
     CDS             <1..321
                     /locus_tag="VGM46_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEY4193702.1"
                     /translation="EDRGPMVIDPGVKVYQGMIIGIHSRDNDLEVNVLKGKKLTNIRA
                     AGKDEAVKLTPPIRMTLERALAWIQDDELVEVTPKTIRLRKLYLDPNERKRFEKSAKV
                     AGAA"
     gene            455..1375
                     /locus_tag="VGM46_13740"
     CDS             455..1375
                     /locus_tag="VGM46_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY4193703.1"
                     /translation="METVTNLKPVTSPLLAILACTLGIFLLSAMDAGVKGLVVVIGAY
                     NALLWRSVLATATAGAVWAAASASRPSAEALSLHVQRGVVITFVALSFFFGLGRLPLA
                     EAIALSFIAPLAALFLAAFLLGEQIGRSAVWASLIGMVGVIVIMAGKFSGGGHAPGAL
                     LGMASIIVSAVFYAYNLILARRQALVAKPLEIVFFQNLIVGVTLCLAFPWLGMTPPPD
                     QWPSLALVTALALFGHALMSWSYARAQAQYLIPTEYSAFVWAVMLGWFFFHEAVGWST
                     LAGAALIIVGCLLASWTKPRLAEPIEIAAI"
     gene            1517..>2578
                     /locus_tag="VGM46_13745"
     CDS             1517..>2578
                     /locus_tag="VGM46_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HEY4193704.1"
                     /translation="MASVKAASKPKKAGGPKKDLSSIEKPATFTEYLLARAPAEDVAA
                     YAAPDLERAAELAGKAVARHKKGASVVAIDADSGVMRDGRPVTVVTVVNDNMPFLFDS
                     ILGEITEDFSEPYLVTHPVIAVRHGKGGVAEILGDGGFARNDVSLDRLSVVHVHIGRL
                     SEEQAGALAARLEKVVAQVRAAVNDWKPMLARLDQAVSEFRYSKVPLDKGHVTEAIAF
                     LEWLRDDNFTFLGMREYKYSGGEKTGTLEREDGPGLGILTDPEVLVLRRGTEAVTTTP
                     EIRAFLHGPEPLIVTKANAKSVVHRRIYLDYIGIKTYTAKGVLSGELRIVGLFTSTAY
                     TRSVMKIPYLRSKAQSVIAK"
BASE COUNT          455 a          853 c          791 g          479 t
ORIGIN      
        1 gaagatcgcg gcccgatggt catcgatccg ggcgtcaagg tctatcaggg catgatcatc
       61 ggcatccaca gccgcgacaa cgaccttgag gtcaacgtgc tgaagggcaa gaagctcacc
      121 aacatccgtg ccgccggcaa ggatgaagcg gtgaagctga ccccgccgat ccgcatgacg
      181 ctggaacgcg cgctggcctg gatccaggac gacgaacttg tcgaggtgac gccgaagacc
      241 atccggctgc gcaagctcta tctcgacccc aacgagcgca agcgcttcga gaagtcggcc
      301 aaggtagccg gcgcggcctg atctgcgcgc cgcgaaaaag cggcgctttc gaccggcaag
      361 cagggcgtca tcggtctgga ccgtgggatt ggctcgacct gcgccctagg cacgtcgcgt
      421 gattcgatct aaccgccgct atagtggcag ccggatggaa accgtgacga acctgaaacc
      481 agttacatcg cccctgctgg ccatcctggc ctgcacgctg gggattttcc ttctgtccgc
      541 gatggatgcg ggcgtgaagg gcctcgtggt ggtgatcggc gcctataacg cccttttgtg
      601 gcgctccgtc ctcgccaccg ctacggccgg cgcggtgtgg gccgccgcat ccgcttcccg
      661 ccccagcgcc gaggcgctaa gccttcatgt ccagcgcggc gtggtcatca ctttcgttgc
      721 gctaagcttc ttcttcggcc tcggccgcct gcccttggcg gaagcgattg cgctttcctt
      781 catcgcgccg ctggcggccc tgttcctggc cgccttcctg cttggcgagc agatcggccg
      841 ctccgccgtg tgggcttcgc tgatcggcat ggtcggggtc atcgtcatca tggccggcaa
      901 gttcagcggc ggcggccatg ctcccggcgc cctgctcggc atggccagta tcatcgtctc
      961 ggccgtcttc tacgcctata atctcatttt ggcgcgccgg caggcgctgg tcgccaagcc
     1021 gcttgaaatc gtgtttttcc agaacctcat cgtcggcgtc acgctctgcc tggcctttcc
     1081 gtggctgggc atgacgccgc cgcccgacca gtggccgtca ctcgccctgg tgacggcgct
     1141 ggcgctgttc ggccacgccc tgatgagctg gtcctatgcg cgggcgcagg cgcaatacct
     1201 gatcccgacg gagtattccg ctttcgtctg ggcggtcatg ctggggtggt ttttcttcca
     1261 cgaagcggtc ggctggtcca ccttggcggg cgctgccctg atcatcgtcg gctgcctgct
     1321 tgcctcctgg acgaaacccc ggctggccga accgatcgag atcgccgcga tctaaccgga
     1381 agccgccacc caaaagaaaa caggggcccg aaatccgatc cttatttatc tcggccagct
     1441 gtcaaaccat tgtcgccaaa ctcggttcat ctggttacga tacgacaaag catcggtcgc
     1501 gggagaattc agcgtcatgg ctagcgtgaa ggcagcgagc aaaccgaaaa aagccggcgg
     1561 gccgaagaaa gacctttcca gcatcgaaaa acccgccacc ttcacggaat atcttctcgc
     1621 ccgcgcgccg gccgaagacg tcgccgccta cgccgcgccc gacctggagc gcgccgccga
     1681 gctggccggc aaagcggttg cccggcataa aaagggcgca agcgtggtcg ccatcgacgc
     1741 cgattcgggc gtcatgcgcg acggccgccc ggtaacggtc gtcaccgtcg tcaacgacaa
     1801 catgccgttc ctgttcgatt cgatcctcgg cgaaatcacc gaggattttt ccgaacccta
     1861 tctggtcacc catccggtca tcgcggtccg ccacggcaag ggcggcgtcg cggagattct
     1921 cggcgatggc ggctttgcca ggaacgacgt ctcgcttgac cgcctgagcg tcgttcatgt
     1981 ccatatcggg cgcttgagcg aggaacaggc aggcgcgctg gcggctcgtc tggaaaaggt
     2041 ggtggcgcag gtgcgcgccg ccgtcaacga ctggaagccg atgcttgcgc ggctcgacca
     2101 ggccgtatcc gagttccgct attcgaaggt gccgctcgac aagggccacg tcacggaagc
     2161 gatcgctttc ctcgaatggc tgcgcgacga caacttcacc ttccttggca tgcgcgaata
     2221 caaatatagc ggcggcgaaa agaccggcac gctggagcgc gaagacgggc cgggcctcgg
     2281 cattctcacc gatcccgagg tgctggtgct gcggcgcggc accgaagcgg tgacgacgac
     2341 gcccgagata cgcgcctttc tgcacggccc cgagccgctg attgtcacca aggccaacgc
     2401 caaatcggtg gtccatcgcc gcatctatct cgattatatt ggcatcaaga cgtatacggc
     2461 caagggcgtc ctgtcgggcg aactgcgcat cgtcggcctg ttcacctcca ccgcctacac
     2521 gcgctcggtg atgaaaatcc cctatctgcg ctccaaggcg cagagcgtca tcgccaag
//
LOCUS       DASYCS010000447         4350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3904178,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000447 DASYCS010000000
VERSION     DASYCS010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4350
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3904178"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="VGM46_13750"
     CDS             <1..487
                     /locus_tag="VGM46_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017267500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEY4193705.1"
                     /translation="LLIAEARKDGGGPVGSRALMSLRVEKGYGSWSREYSPEYWPQEV
                     HMDRLVKMDKGDFLNRDAYEAIAGNAPRDTMVMIEIEADGADATGGEPVFLPDGTPAG
                     HVTSGTYGYSVGKSLALGYLKTPLATPGAEVSVAILGRPTKARVLEQAPFDPEGKRLR
                     A"
     gene            complement(536..1498)
                     /locus_tag="VGM46_13755"
     CDS             complement(536..1498)
                     /locus_tag="VGM46_13755"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEY4193706.1"
                     /translation="MNTVSTDRANPTLAKLLTPRARTFLGIPAATEARAGSAAIFGAV
                     HGTPYGACGLQNGREQGPDAVRRAITTASVHIDHWDFDFDGPLLNDGALDAFDLGDVV
                     TMPDDAAANRRNIEAVTRAVRTAGAVPLLIGGDDSVAIPFMRGLDGGEPLHILQIDAH
                     IDWRDDIDGEKLGYSSTMRRASELSCAASMTQAGIRAVGSARRGEVETARAWGSRLVT
                     VSEARRLGMDAVAEAIPRGGSLLIHIDCDAMDPGICPGVNAMSPGGFAFHEMTELIAA
                     AMRGRKLAGFSIVEFQPAADINDMSAAIVGRLVSHVLGHLARAG"
     gene            1749..3107
                     /locus_tag="VGM46_13760"
     CDS             1749..3107
                     /locus_tag="VGM46_13760"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEY4193707.1"
                     /translation="MIDEKVARDFLDKNPDIEVLEAFVIDVNGVPRGKWIPRERSVDV
                     LVNGMAMPRSVYALDVWGRDVNAAGLAEGTGDPDGLCVPVPETLSRISWLKRPTAQVM
                     LSMKGPGGGHFFADPRQVLANVLDRFHQMKLTPVVATELEFYLIDPVRSALDPVRPPN
                     TREGRWHTGQTQVLSIAELQDFEDVFYEIDATCREQGVPADTTLRENGPGQYEINLNH
                     VPDALRAADHAVLLKRIVKGVARKHELDATFMAKPYGMQAGNGMHVHFSMLDDNGRNV
                     FVGDNGPSDLLMQSVAGLLRYMGESTAVFAPHANSYRRLKPSEHAPTFASWGFDNRSA
                     AVRVITASRPATRIEHRVAGADSNPYLVLAMILQAAYMGMKEKLDPGGPISGDEHALD
                     NEPLQTSWDSAVQRFATSGFAYGAVGPEFRSLYTACKRQELDEFSLRVTDVEYDAYIR
                     MA"
     gene            3179..3883
                     /locus_tag="VGM46_13765"
     CDS             3179..3883
                     /locus_tag="VGM46_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006206627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY4193708.1"
                     /translation="MDIRNPASEFIFEPPARPTLPVIGTQAVFPVRRVYCVGRNYAAH
                     AVEMGHDPDKDPPFFFQKNPDNLDTSGRFPYPPASSDVHHEIEMLVALKSGGADIALD
                     KALDCVFGYGVALDMTRRDLQARAKDMGRPWEVGKAFEASAPCTPLVSVSDVGHPAQG
                     AIWLDVNGERRQTGDLNQMIWKVPEMISYLSGLFTLMPGDVILSGTPSGVGPVQRGDV
                     LTGHVDGVGDLEVHVL"
     gene            complement(3898..>4350)
                     /locus_tag="VGM46_13770"
     CDS             complement(3898..>4350)
                     /locus_tag="VGM46_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006468294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY4193709.1"
                     /translation="VGLILAWNQPALPFSPYNTPLILLLAYCCILLPHPVRYTHAALL
                     QIGDNLEAAARVAGATGMTTLRRILLPLIMPSLVSSMLLVFAVASRELVASILIAPVG
                     MPTVATFIWRQFEQGSIGLGMAMALIAILITTLLPLALLGLMRRAPMM"
BASE COUNT          794 a         1382 c         1422 g          752 t
ORIGIN      
        1 actgctgatt gccgaagccc gcaaggatgg cggcggcccg gtcggctcgc gggcgctgat
       61 gtcgctgcgc gtcgagaagg gctatggcag ctggagccgc gaatactcgc cggaatattg
      121 gccgcaggag gtccacatgg atcgtctggt caagatggat aaaggcgatt tccttaaccg
      181 cgatgcctac gaggcgattg ccggcaatgc gccgcgcgac acgatggtga tgatcgagat
      241 cgaggcggac ggcgccgatg ccaccggcgg cgagccggtc ttcctgccgg acggcacgcc
      301 tgccgggcac gttacttccg gcacctatgg ctactcggtc ggcaagtcgc tggcgctggg
      361 ctacctcaag acgccgttgg ccacgcccgg cgccgaagtg tcggtcgcca tactgggccg
      421 gccgaccaag gcgagggttt tggagcaggc tccgttcgat ccggaaggaa agcgcctgcg
      481 cgcctgatgg aacgggcgtt cctggtcgag aacgcccgtt cagcctatcc gtcaatcaac
      541 cggcgcgggc cagatggccc aacacatggc tcaccagcct gccgacgatt gccgccgaca
      601 tgtcgttgat atcggcggcc ggctggaatt cgacaatgga aaagccggct aactttctgc
      661 cgcgcatggc ggccgcgatc agctcggtca tctcatggaa ggcaaagccg ccgggcgaca
      721 tggcgttgac gcccgggcag atgccggggt ccatcgcatc gcagtcgatg tggatgagca
      781 gcgatccgcc gcgcgggata gcctcggcaa cggcgtccat gccaaggcgt cgcgcttcgc
      841 tgacggtgac cagccgcgat ccccacgccc tggcggtttc gacctctccc cgccgcgcgc
      901 tgcccaccgc gcggatgccg gcctgcgtca tggaagctgc gcatgaaagc tccgatgcgc
      961 gccgcatggt gctggaatag ccgagcttct cgccgtcaat gtcgtcgcgc cagtctatat
     1021 gcgcgtcgat ctggagaata tgaagcggtt cgccgccgtc gaggcccctc atgaagggta
     1081 tggccacgga atcgtcaccg ccgatcaaaa gcggcaccgc gccggcagta cgcaccgcgc
     1141 gcgtcacggc ttcgatgttg cgccggttgg cggccgcgtc atccggcatg gtgacgacat
     1201 cgccgagatc gaaagcatcg agcgcgccgt cgttcaacag cggcccgtcg aagtcgaaat
     1261 cccaatggtc gatatgcacc gatgccgtcg tgatggcgcg gcgaacggca tccggcccct
     1321 gttccctgcc gttttgaagc ccgcaggcac cataaggcgt tccatgaacc gcaccgaaaa
     1381 tcgccgccga tccagcccgg gcctcggtcg ctgccggtat tccgagaaag gttcgggcgc
     1441 ggggcgtcag caatttcgcc agcgtgggat ttgcgcgatc tgtcgagact gtattcatgc
     1501 aaccacctaa ggaacgcggc gtcgccctgt caattcggcc ggcgctgccg taaacagatc
     1561 gacttgggtc aggccgcgct tgcccctcgc ttcttggcct cgtttctggc accttccggt
     1621 ggattgaggg gccgggcctg ataaatcatc gacttggcgg atggcaaatg cccggctggg
     1681 cagtttgccg gcgccgttcg acggcgcggt gaaatgcgga acgaaatttc cgcaaaacat
     1741 ggtggatcat gattgacgaa aaggtcgcgc gcgactttct agacaaaaac ccggacatcg
     1801 aggtgctgga agccttcgtc atcgacgtga acggcgtgcc acgcggcaaa tggataccgc
     1861 gcgaacgctc ggtggacgta ttggtcaacg gcatggcgat gccgcgctcc gtctatgcgc
     1921 tcgacgtgtg ggggcgggac gtcaacgccg ccggcctcgc cgaaggcacc ggcgatcccg
     1981 atgggctttg cgtgccggtt cccgaaacgc tgtcgcggat aagctggttg aagcgtccga
     2041 ccgcgcaggt gatgctgagc atgaaaggac cgggcggggg gcatttcttc gccgatccgc
     2101 gccaggtgct ggccaatgtg ctcgaccgtt tccaccagat gaagctgacg ccggtcgtcg
     2161 ccaccgagct tgaattctac ctgatcgatc cggtgcgttc cgcgctcgat ccggtgcgcc
     2221 cgcccaatac gcgcgaaggc cgctggcaca ccgggcagac gcaggtgctg tcgattgccg
     2281 aattgcagga tttcgaggac gttttctacg agatcgacgc cacctgccgg gagcagggcg
     2341 tgcccgccga caccacgctg cgcgaaaacg ggccgggcca gtatgagatc aacctcaacc
     2401 acgttcccga tgcgctgcgc gccgccgacc atgccgtgct gttgaagcgc atcgtcaagg
     2461 gcgtcgcgcg caagcatgaa ctcgacgcga cgttcatggc caaaccctac ggcatgcagg
     2521 ccggcaacgg catgcatgtg catttttcca tgctggatga caacggccgc aatgtcttcg
     2581 tcggcgacaa tggcccatcc gatttgttga tgcagtcggt cgccgggctg ctgcgctata
     2641 tgggcgagag cacggcggtt tttgccccgc atgccaactc ctaccggcgc ctgaagccca
     2701 gcgagcacgc gccgaccttc gcctcctggg gcttcgacaa ccgctcggcc gccgtgcgcg
     2761 tcatcaccgc cagccggccg gcgacccgca tcgagcaccg cgtcgccggc gccgacagca
     2821 atccctatct ggtgctcgcc atgatccttc aggccgccta tatgggcatg aaggaaaagc
     2881 tcgatcccgg cggcccgatc agcggtgacg aacatgcgct ggacaacgag ccgctgcaaa
     2941 cgagctggga cagcgcggtg cagcgcttcg ccacttccgg ctttgcctat ggcgcggtcg
     3001 gccccgaatt ccgctcgctc tacacggcct gcaagcgtca ggagctggac gagttttccc
     3061 tgcgcgtcac cgatgtcgag tatgacgcct atatccggat ggcgtagcgc ggccggattg
     3121 aacatcgccg tccggtgagc gagcgcgccg gcgcgagacg actatgcgag gaaagatcat
     3181 ggatatccga aatcccgcca gcgaattcat tttcgagccg cccgcccggc ccacgcttcc
     3241 ggtgattgga acgcaagccg tgtttccggt gcgccgggtc tattgcgttg gccgcaacta
     3301 tgccgcgcac gccgtcgaga tgggccacga cccggacaag gacccgccgt tcttcttcca
     3361 gaagaacccg gataatctgg ataccagcgg ccgtttccct tacccaccgg cgtcgagcga
     3421 tgttcaccac gagatcgaaa tgctggtggc gctgaagagc ggtggagcgg acatcgcgct
     3481 cgacaaggcg ctggactgcg tcttcggcta tggcgtcgcg ctcgacatga cgcggcgcga
     3541 tcttcaggcc agggcgaagg acatgggccg gccatgggag gtcggcaagg ccttcgaagc
     3601 ctccgcgccc tgcacgcctc tggtttccgt gtcggatgtt ggccatcccg cgcagggcgc
     3661 gatctggctg gacgtcaatg gcgagcgcag gcagaccggc gacttaaacc agatgatctg
     3721 gaaggtgccg gaaatgattt cctatctttc cggcctgttc acgctgatgc ctggcgacgt
     3781 catactgtcg ggcaccccct ccggtgtcgg tccggtccag cgcggcgatg tccttacggg
     3841 acacgtcgat ggcgtcggcg atctggaagt gcacgtctta tagaccgccg cgcgccctca
     3901 catcatcggg gcgcggcgca tcaggccgag caatgcgagc ggtagaagcg tcgttatcaa
     3961 gatggcgatc aaggccatgg ccatgccgag gccgattgaa ccctgttcga actgccgcca
     4021 gatgaaggtc gccaccgtcg gcatgccgac cggcgcgatc aggatggagg cgaccagttc
     4081 gcgcgaggct accgcaaaca ccagaagcat cgacgagacc aggcttggca tgatcagcgg
     4141 cagcaggatg cggcgcagcg tggtcatgcc ggtcgcgccg gcgacgcggg ccgccgcttc
     4201 gagattgtcg ccgatttgca gcagcgccgc gtgcgtatag cgcaccggat gcggcagcag
     4261 gatgcagcaa taggcgagca acagaatcag cggggtattg tagggcgaga acgggagggc
     4321 cggctggttc caggcgagga tcagcccaac
//
LOCUS       DASYCS010000448         7975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2890093,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000448 DASYCS010000000
VERSION     DASYCS010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 7975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7975
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2890093"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VGM46_13775"
     CDS             <1..199
                     /locus_tag="VGM46_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HEY4193710.1"
                     /translation="VRGLVAIMLALFSGQKASTILETDADAVLKTLGLDEHLSPQRAN
                     GLRSMVKRIKRDAEASLKQVA"
     gene            complement(220..1020)
                     /locus_tag="VGM46_13780"
     CDS             complement(220..1020)
                     /locus_tag="VGM46_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6456 domain-containing protein"
                     /protein_id="HEY4193711.1"
                     /translation="MDIPASRRRILHFLSSGPASVRPASRDGSILLEATGRGTIAVDA
                     AVLSSLASGGILVRASHSVALSGEGAALAARLASPSGVRQEQHRDIELVTVAMPEGPA
                     TVLANMAESPLAQLVRRKTRDGKPFLNSGEFQAGERLRADYTRGQIMPRLGANWTAGV
                     SSGRRAAGGGVADLTDTALAARQRVDNALSAVGPELSGVLVDVCCFLKGLELVEAERG
                     WPVRSAKIVLKTALGVLARHYSPADRTQNRGQRHPLHWGAQDYRPTIS"
     gene            complement(1034..1453)
                     /locus_tag="VGM46_13785"
     CDS             complement(1034..1453)
                     /locus_tag="VGM46_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009452161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY4193712.1"
                     /translation="MQQIQSAVPKQPAQTSSPDEAATPAFCPTAKRRDEVAIEMCECL
                     IDIAAALFSVPSKELRKPGRTPVPVTRVRQICMYVAHVVLRLTMGDVGVGFGRDRTTV
                     VHACQVIEDLRDDPEFDQVVSMIERVAFAAFRNRLGL"
     gene            1663..2049
                     /locus_tag="VGM46_13790"
     CDS             1663..2049
                     /locus_tag="VGM46_13790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193713.1"
                     /translation="MLRFQEEAQLFGATKLAATARAQQIAAMQLVCGIDLSAALRAGP
                     DAQGQIICRVERVIERERLKGMRRHWSYDLNRHIALKQVLDQLRKLQPEGDASPAKKK
                     LRRTVSTNGNGARRRRQNRKILGKDG"
     gene            complement(2046..2474)
                     /locus_tag="VGM46_13795"
     CDS             complement(2046..2474)
                     /locus_tag="VGM46_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HEY4193714.1"
                     /translation="MDTADSPSRNSEMLIELTADVVSAYVSNNPVPVGELPNLIADVH
                     AALGRVGSASEPPSVDKQKPAVSPKRSVHDDFIICLEDGKKFKSLKRHLMTHYALTPD
                     QYREKWGLEATYPMVAPNYASARSALAKKMGLGRKRKTVR"
     gene            3000..5108
                     /locus_tag="VGM46_13800"
     CDS             3000..5108
                     /locus_tag="VGM46_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEY4193715.1"
                     /translation="MTRPAAFPSIRPPHAEKHSVSDTRHGITRTDDYAWLRAENWQEV
                     FRDPTLLDPAIRRHLEAENSYQSALMADTADLQRKLFGEMKGRIKEDDSSVPMNDGPY
                     AYGSSFKLGGEQPRYFRTPRDGGAETILLDGDAEAAGKPYFQLGGIDHSGDHQRLLWA
                     FDDKGSEFYTLRIRDIAGQADLADEVADTGGGGVWDAADRGFFYTRLDPNHRPSKVLY
                     HVLGQDPANDRLVYEETDPGFFMSVGGTRSNEWIMIGINDHETSEYRIFRASDPDAEP
                     KVVAARETGMQYDLEEGGEVFFILTNADGAKDFKIMTAPVADPARANWKELVGHEPGR
                     LILSVMGFKDHMVRLERKDGLPRIVIRELASGEEHSISFAEEAFSLGLSGSYEYDTET
                     IRFSYSSMTTPAQLFDYNMRTRQRRLLKTQEVPSGHDADRYVTRRLMAPAPDGELVPV
                     SLLYRKDTPLDGSAPCLLYGYGSYGITIPASFNTNSLSLVDRGFVYAIAHIRGGKDKG
                     YGWYDDGKRENKMNTFTDFIAVARHLVGQNYTAHDRIVAQGGSAGGMLMGAIANMAPD
                     AFGAIIAEVPFVDVLTTMLDDTLPLTPPEWPEWGNPIVSQKDYQTIAAYSPYDNVAAR
                     AYPPILAQAGLTDPRVTYWEPAKWVARLRDNNTTDNQVLFKINMDAGHAGASGRFSRL
                     EEIAFTYAFALKVTGKAELS"
     gene            complement(5115..6062)
                     /locus_tag="VGM46_13805"
     CDS             complement(5115..6062)
                     /locus_tag="VGM46_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY4193716.1"
                     /translation="MRLLKKLAGTALVVACGAALGGWFLSAPSTLDAQTLAQLGPGDA
                     AKGRRIFYAGGCSSCHARPDAKGDARLELPGGMELKTAFGTFVPPNISQDKADGIGDW
                     SVQDLANAMLKGVSPSGEHLYPAFPYASYARMKPSDIGDLYAFLKTLPAIAGKAPANS
                     LHFPFNIRRGIGLWKRLYLGSDPVVPFPDGTSQQVLAGRYLVEGPGHCGECHTPRDLA
                     GGTNKGAWLAGAVAAEGEGIVPNITSGEGGIGEWSAADIANYLETGFTPDFDTVGGAM
                     VDVQANIAELTPQDRAAIAAYLKAIPPHPNGYPARKPAS"
     gene            complement(6073..6510)
                     /locus_tag="VGM46_13810"
     CDS             complement(6073..6510)
                     /locus_tag="VGM46_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY4193717.1"
                     /translation="MRKLILAISLLALSGSAALADQIQDRQAIMKERGKIVGGLAKIV
                     KGEQEFDAAAVMTALQALQVNAEKFDVDALFPAGSDKGDTTASPKIWEDMAGFKAAED
                     KYLADVKAANAAAPADVDALKTQLGILGGDCGTCHKAYRVKKG"
     gene            complement(6675..7277)
                     /locus_tag="VGM46_13815"
     CDS             complement(6675..7277)
                     /locus_tag="VGM46_13815"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006700042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEY4193718.1"
                     /translation="MAFELPALPYDYEALQPYMSKETLEYHHDKHHKAYVDNGNKLAA
                     EAGMGDLSVEEVVKQSFGKNAGLFNNAAQHYNHVHFWKWMKKGGGGNKLPGALQKAVD
                     SDLGGYDKFKADFIAAGTTQFGSGWAWLSVKDGKLAISKTPNGENPLVHGASPILGVD
                     VWEHSYYIDYRNARPKYLEAFVDSLVNWDHVLELYEKAAG"
     gene            complement(7379..>7975)
                     /locus_tag="VGM46_13820"
     CDS             complement(7379..>7975)
                     /locus_tag="VGM46_13820"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEY4193719.1"
                     /translation="AVYIRPMYWAEQGGYMGVPADPASTRFCLCLYESPMIAPAGFSV
                     TLSPFRRPSLETMPTNAKAGCLYPNNGRAIMEARMRGFDNALVLDMLGNVAETASSNV
                     FLVKDGHVFTPAANGTFLSGITRARTMALLGEYGFQTTEKTLSVRDFLEADEIFSTGN
                     HSKVVPVIRIEERSLQPGPVAKKARELYWEWAHSTSAG"
BASE COUNT         1564 a         2491 c         2404 g         1516 t
ORIGIN      
        1 cgtgcgcggc ctcgtcgcca tcatgctcgc cctgttttcc gggcaaaagg cgagcacgat
       61 tctagagaca gacgctgatg cggtgctgaa gacgcttggc ctagacgaac atctttctcc
      121 gcagcgcgcc aacggcctgc gttcgatggt aaagcgcatc aagcgggatg cggaagcctc
      181 gctgaagcag gttgcctgat cggtctggcg cgcgggcgtt cacgaaatcg tcggccgata
      241 gtcctgcgcg ccccaatgca atggatggcg ctggccgcgg ttttgcgtcc tgtcggcggg
      301 cgaatagtgc cgcgccagca cgcctagcgc cgttttcaag acgatcttgg ctgaacgcac
      361 cggccagccg cgctccgctt cgaccagttc gaggcctttg aggaagcagc agacgtctac
      421 cagcacgccg gaaagctcgg ggccgacagc cgatagcgca ttatccaccc gctggcgcgc
      481 agccagcgcg gtatcggtca ggtcggccac tccgccaccg gcggcgcgcc tgcccgagga
      541 gacgccagcc gtccagttcg cgccaagcct cggcatgatc tggcctctcg tataatcggc
      601 acgaagtcgc tctcccgcct ggaactcgcc agagttgagg aacggtttgc catccctcgt
      661 cttgcggcga accagttggg caagcggtga ttccgccatg ttggccagca cggtggctgg
      721 accttccggc atcgccacgg tgacaagctc gatgtcgcgg tgctgctcct ggcgcacgcc
      781 cgagggcgag gccagacgcg cggccaaggc cgccccctcg cccgacagcg cgacactgtg
      841 cgaagcccga accaggatgc cgcccgaggc gagcgacgac agcacggcgg catccaccgc
      901 aatggttccg cgtcccgtcg cctccagcaa tatactgccg tcgcgcgagg ccgggcgcac
      961 ggaggcgggg ccgctggaca aaaagtgaag gatgcgacgg cgcgatgccg ggatgtccat
     1021 gcgctgctcc atttcaaagg ccaagccggt tgcgaaacgc ggcgaaggct acccgctcga
     1081 tcatcgacac gacctgatcg aactccggat cgtcgcgcag gtcctcgatc acctggcaag
     1141 cgtgaaccac cgtcgtgcgg tccctgccga aacccacccc gacatcgccc atcgtcagcc
     1201 gcagcaccac atgcgcaaca tacatgcaga tctggcgaac gcgggtgacc ggcaccggcg
     1261 ttcttccggg cttgcgcaat tccttgctcg gcacggagaa aagcgctgcc gctatatcga
     1321 tcaggcattc gcacatttcg atcgcgacct cgtcgcgacg cttggccgtc ggacaaaaag
     1381 cgggcgttgc cgcttcgtcc ggcgacgagg tctgcgccgg ctgtttcggc acggctgatt
     1441 gaatttgctg cacgtgtggc cctccgcata taggaatatg ttccttatac cgcagacccc
     1501 tgacatgtga acaaacaaag aaccattgcg caacagctta ttttacaagc ccttgataaa
     1561 catgtgtttt caattttttc taggtaaatc cgcatccgga tttctcccca ccgcccggcg
     1621 agcctcgata gtggaaacac aaattccaac cgagagaagc ccatgctgcg ttttcaggaa
     1681 gaagcccaac tcttcggcgc cacgaaattg gcggcgaccg cacgcgcgca acagattgcc
     1741 gcaatgcagt tggtgtgcgg catcgacctt tcagcggcgc tccgggctgg cccggacgcg
     1801 cagggccaga tcatctgccg ggtcgagcgg gtgatcgagc gcgaaaggct gaagggaatg
     1861 cggcggcact ggagctacga tctcaatcgc cacatcgccc tcaagcaggt gctggaccag
     1921 ttgcgcaaat tgcaaccgga aggcgatgcg agcccggcca aaaagaaact gcgccgaacg
     1981 gtttccacca atggaaacgg cgcccgcagg cgccgtcaaa atcgcaaaat cctcgggaag
     2041 gacggttagc gcaccgtctt gcgcttgcgg ccaaggccca tcttcttggc cagggccgag
     2101 cgggcggagg cgtagtttgg agcaaccatc ggataggtcg cctcaaggcc ccacttctcg
     2161 cgatactggt cgggcgtcag cgcataatgg gtcatcagat ggcgcttcaa cgatttgaac
     2221 ttcttgccgt cttccagaca aatgataaaa tcgtcgtgga ccgagcgctt cggactgacc
     2281 gcaggcttct gcttgtccac cgaaggcggc tcgctggcgc ttccaactcg tcccagggcc
     2341 gcatgaacat cggcgatcaa gttcggaagt tcaccgactg gaaccggatt gttgctgaca
     2401 taggcagata ccacgtccgc ggtcagctcg atcaacatct cactgtttct ggaaggcgag
     2461 tcggcagtgt ccatagatgt cgtgtcctag agagagttgc gggagcaacg ctgctttgcg
     2521 tgcctagtat ttaccggcaa atttgcctct taacaccgcc gcgattcgcg ttgcgtcccg
     2581 ttattgccgc aagcgaacat tcaagtcaat tcactgatat tggtacattt tgtaacatta
     2641 atgattcatt ccgagctgat cacaatttaa acatacctta tgcaacttct acgacaatat
     2701 ccaaatcaga cgacacgtca tcgaatagat cacgtcatat tatatccttt gacgacatat
     2761 atccgcctcg tatgcaccac accgtctcat atgccgcgac acttgcggac accgctgttt
     2821 gcctgcatcc ggccaatcat tcaccggata ttgcgttgcg acggcgaggc caccaccttt
     2881 aatgcggttg cacgccatgg gtgacatcga ggtcgagcgg caattcagcc gtggctcttg
     2941 aagtccgccc gcaagacgtg ctcactcgaa ccgacagatt cattgccttg cgagatttca
     3001 tgacccggcc agcagccttc ccgtccatcc gcccgcccca tgccgaaaaa cacagcgttt
     3061 ccgatacacg gcacgggatt acccgcacgg atgactatgc gtggcttcgg gcggaaaact
     3121 ggcaggaagt gtttcgggat cccacgctgc tcgacccggc catccgccgc catctggaag
     3181 cggagaacag ctatcagtcg gcgctgatgg cggacacggc cgatcttcag cgcaagctgt
     3241 ttggcgagat gaaggggcgc atcaaggagg acgattcgtc ggtgccgatg aatgacggcc
     3301 cctacgccta cggctcatcc ttcaagcttg gcggcgagca gcctcgctat ttccgcacgc
     3361 cgcgtgatgg cggcgccgag acgatcctgc tcgacggcga cgccgaagcg gcgggcaaac
     3421 cctatttcca gcttggcggc atcgaccatt cgggcgatca ccagcgcctg ctctgggcct
     3481 tcgacgacaa ggggtccgaa ttctacacgc tgcggatacg cgatattgcg ggccaggcag
     3541 accttgccga tgaagttgcc gatacgggcg gcggcggcgt gtgggacgcg gctgaccgcg
     3601 gcttcttcta cacgcgcctc gaccccaatc accggccttc gaaggtgctc tatcatgtcc
     3661 ttggccaaga cccggccaac gaccggctcg tctacgagga aaccgacccc ggtttcttca
     3721 tgagcgtcgg cggcacgcgc agcaatgaat ggatcatgat cggcatcaac gatcatgaaa
     3781 cctcggaata ccgcatcttc cgggcgtccg atcccgacgc cgaaccgaag gtcgtcgccg
     3841 cgcgcgaaac cgggatgcaa tacgacctcg aagaaggcgg cgaggtcttt ttcattctaa
     3901 ccaatgccga tggcgccaag gatttcaaga tcatgacggc tcccgtcgcc gatccggcgc
     3961 gcgccaactg gaaagagctt gtcggccatg agccgggccg gcttatcctt tcggtgatgg
     4021 gcttcaagga ccacatggtc cggctggagc gcaaggacgg cctgccgcgc atcgtcatcc
     4081 gcgaactcgc cagcggcgag gaacattcga tttccttcgc ggaggaggct ttctcgctgg
     4141 gcctgtcggg ttcttatgaa tacgacaccg agacgatccg cttttcctat tcgtccatga
     4201 cgacgccggc gcaattgttc gactacaaca tgcgcacgcg ccaacgcagg ctgctgaaga
     4261 cgcaggaagt gccttccggc cacgatgccg accgctatgt cacccgccgc ctgatggcgc
     4321 cggcgcccga tggcgaactg gtgccggtgt cgctgctcta ccggaaggat acgccgctcg
     4381 acggatcggc gccgtgcctt ctctatggct acggctcata cggcatcacg atcccggctt
     4441 ccttcaacac caatagcctg tcgctggtcg atcgcggctt cgtctacgcc atcgcgcaca
     4501 tacgcggcgg caaggacaag ggctatggct ggtacgatga cggcaagcgc gaaaacaaga
     4561 tgaacacctt caccgacttc atcgccgtgg cgcgccacct tgtcgggcag aactacaccg
     4621 cgcatgaccg catcgtcgcc cagggcggct cggccggcgg catgctgatg ggggctatcg
     4681 ccaacatggc tcccgatgcc ttcggggcga tcatcgccga ggtgcctttc gtggacgtgc
     4741 tgaccaccat gcttgacgac accctgccgc tgacgccgcc ggaatggccc gaatggggca
     4801 atccgatcgt gtcgcaaaag gactaccaga ccatcgcggc ctactcgccc tatgacaatg
     4861 tcgcggcgcg cgcctatccg ccgatcctgg cgcaggccgg cctgaccgat ccgcgagtca
     4921 cttattggga gccggccaaa tgggttgccc ggttgcgcga caacaacacg accgacaatc
     4981 aggtcctgtt caagatcaac atggatgccg gccatgccgg cgcgtccggg cggttctcgc
     5041 ggctggagga gatcgccttc acctacgcct tcgcgctcaa ggtgacggga aaggccgagc
     5101 tttcctgaag ggggtcaact cgccggtttg cgcgcgggat agccgttggg atgcggcggt
     5161 atcgccttga gataggcggc gatcgcggcg cgatcctgcg gcgtcaactc ggctatattg
     5221 gcctgcacgt cgaccatcgc gccgccgacc gtatcgaaat cgggcgtgaa accggtttcg
     5281 agataattgg cgatatcggc tgccgaccac tcgccgatgc cgccctcgcc cgaggtgatg
     5341 ttgggaacga tgccttcgcc ttcggcggca accgcgccgg ccagccatgc ccccttgttc
     5401 gtgccgccgg cgaggtctcg cggcgtatgg cattcgccgc agtggcccgg tccctcgacc
     5461 agatatcggc ctgccagcac ttgttgtgac gtgccgtcgg gaaacggcac gaccggatcg
     5521 ctgcccagat aaagccgttt ccacaggccg atgccacggc ggatgttgaa cggaaaatgg
     5581 agcgaattgg cgggtgcctt gccggcaatc gccggcaggg ttttcaggaa cgcatagagg
     5641 tcgccaatgt cggatggctt cattcgcgcg taagacgcgt agggaaacgc cggatagaga
     5701 tgctcgccgg aaggcgaaac gcctttcagc atcgcattgg caaggtcctg caccgaccag
     5761 tcgccgatgc cgtcggcttt atcctgcgat atattgggcg gcacaaaagt gccgaacgcc
     5821 gttttcagtt ccatgccgcc tggaagttca agccgggcat cgccctttgc atcggggcgg
     5881 gcatggcagg acgagcagcc gccggcataa aagatgcgcc ggcccttggc tgcgtcgccg
     5941 gggccaagct gcgccagagt ctgcgcatcg agcgtcgaag gcgcggacag gaaccagccg
     6001 ccaagcgctg cgccgcacgc cacgacaagg gcggtgccgg cgagcttttt cagcaggcgc
     6061 attcgggcgg aatcagccct ttttcaccct gtatgccttg tggcaggtgc cgcaatcgcc
     6121 acccaggatg ccaagctgcg tcttgagcgc gtcgacatcc gccggtgcgg cggcgttggc
     6181 ggctttcaca tccgccagat atttgtcttc ggcggccttg aagccggcca tgtcttccca
     6241 aatcttcggc gacgcggtgg tgtcgccctt gtcgctgccg gcggggaaaa gcgcatcgac
     6301 gtcgaacttc tcggcattga cttgcagcgc ctgcaaggcg gtcatcaccg cggctgcgtc
     6361 gaattcctgc tcgcccttca cgatcttggc cagaccgccg actatcttcc cgcgctcctt
     6421 cataatggcc tgacgatcct ggatctggtc ggccagggct gccgatccgc ttagtgcaag
     6481 caatgagatg gcgaggatga gctttctcat ggtcgtctcc aggttcaaag agttgtcggg
     6541 cttgaatatg aggcacaagg tggctgaaag tttacgtgct ttcgattcac gcttgcgtga
     6601 tctgcaatgg gttcgtcagc cgacgaaaaa gccccggctt caaggccggg gcttcgagct
     6661 ttcgccaagg gcgatcagcc ggcggctttc tcgtaaagct ccaggacgtg gtcccagtta
     6721 acgaggctgt cgacaaaggc ttcgaggtat ttcggacggg cattgcgata atcgatgtag
     6781 taggaatgct cccagacatc gacgccgagg atcggcgacg cgccatgaac cagcgggttc
     6841 tcgccattcg gcgtcttgga aatggcaagc ttgccgtcct tgaccgagag ccatgcccag
     6901 cccgaaccga actgcgtggt gccggcggcg atgaaatccg ccttgaattt gtcgtagccg
     6961 ccaaggtcgc tgtcgacggc cttctgaagc gcgcccggca gcttgttgcc gccgccgccc
     7021 ttcttcatcc acttccagaa atggacgtgg ttgtagtgct gggcagcgtt gttgaacagg
     7081 ccggcattct tgccgaacga ttgcttgacg acttcctcca ccgagagatc gcccatgccg
     7141 gcttcggcag ccagcttgtt gccgttgtcg acatacgcct tgtggtgctt gtcgtggtgg
     7201 tactccagcg tctccttcga catgtagggc tgcaaggcct catagtcata gggcaaggcg
     7261 ggaagttcga aagccatagt gctactcctt ggaaaatccg gtatcgctta ccggacaatg
     7321 atagggcgaa accgatcgca ggcaaggccg gaccgaaggc cgccgtcgtt ctagaccttc
     7381 atccggcgga agtcgaatgc gcccactccc aataaagctc gcgggctttc ttggcgaccg
     7441 ggccgggctg aagactgcgt tcctcgattc ggatcaccgg cacgaccttg gaatggttgc
     7501 cggtcgaaaa gatttcgtcg gcctccagga aatcgcgcac ggacagcgtc ttttccgttg
     7561 tctggaagcc atattcacca agcagcgcca tggtgcgcgc gcgggtgatg ccggaaagaa
     7621 aggtgccgtt ggcggccggg gtgaaaacgt gcccgtcctt gacgaggaaa acgttcgacg
     7681 aggccgtctc ggcgacgttg cccagcatgt ccagcaccag cgcgttgtcg aagccgcgca
     7741 tcctggcctc catgatggcg cggccattat tgggatacag gcagccggcc ttggcgttgg
     7801 tcggcatggt ttccagggac ggacgccgga acggcgacag ggttaccgaa aacccggcgg
     7861 gcgcgatcat cggcgattcg tacaagcaca ggcaaaaccg ggtagaagcc ggatcggccg
     7921 gaacgcccat gtagccgccc tgctcggccc aatacatcgg gcgaatgtag acggc
//
LOCUS       DASYCS010000449         4397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_5061558,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000449 DASYCS010000000
VERSION     DASYCS010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4397
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5061558"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="VGM46_13825"
     CDS             complement(<1..642)
                     /locus_tag="VGM46_13825"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861141.1"
                     /note="One of the components of the high-affinity
                     ATP-driven potassium transport (or KDP) system, which
                     catalyzes the hydrolysis of ATP coupled with the exchange
                     of hydrogen and potassium ions; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit B"
                     /protein_id="HEY4193720.1"
                     /translation="MGGALKPVSWASVIGEAFKKLSPRVQVKNPVMFVVYLGSIITTL
                     LWIQALTGKGEAPAAFIFAIAAWLWFTVLFANAAEALAEGRGKAQADALRATRQRVIA
                     RMLKEPTRESESWPQPSDELDVGAYVLVEAGEIIPADGEVIQGAASVDESAITGESAP
                     VIRESGGDFCSVTGGTRVLSDWIIMRVTARPGEAFLDRMIAMVEGAKRGKTPNE"
     gene            complement(695..1372)
                     /gene="kdpC"
                     /locus_tag="VGM46_13830"
     CDS             complement(695..1372)
                     /gene="kdpC"
                     /locus_tag="VGM46_13830"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF001454.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HEY4193721.1"
                     /translation="MRNEQTIIHTAAPQKAFKGVARPVLASAVFFMLVTGIGYPLATT
                     GVASLLFPDQAKGTLIVKDGKAVGSHHIGQYFSRPEYFHGRPSVTPGTDPNDPSKTVD
                     QPYNAALSAASNQGAISKKLLDAVAQRAKAYRDENGLAADASVPVDAVTASASGLDPD
                     ISVANARLQAARVAGARGLTAEKVMQLLDQNTTGRQFGVLSDPRINVLELNLALDAAV
                     PARPAAQ"
     gene            complement(1386..3089)
                     /gene="kdpA"
                     /locus_tag="VGM46_13835"
     CDS             complement(1386..3089)
                     /gene="kdpA"
                     /locus_tag="VGM46_13835"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HEY4193722.1"
                     /translation="MAQDILQFVLILAIMTGLAVVLGKWMTHVFISPAHNFIERGTYR
                     ILGIDPGEKMSWKRYGIVLVLSNAAMLLLGYFILRIQGFLPGDTLARAAQTPDLAFNT
                     AVSFTTNTNWQAYSGEASLSNFSQMAAITFLMSISATTGVAAAGGFIRGLSRKTTADI
                     GNYWVDFTRVLYRVLLPLCFVMALIYVWQGIPQTLNADAIVTTLEGARQQLILGPVAS
                     LEAIKHIGTNGGGFYGMNAAHPFENPTPLTNTLHILGMLLIPSALTYTFGAMVLRQRQ
                     GWAFFGAFLVMFIGFLVLVYSAEHAGNPLLASMGVDQAQTALQAGGNMEGKELRFGIA
                     QTSMFVTTTTAATTGSVDAMHSSLTPLGGFVTIAQMMLNNVFGGDGVGLINLVTYAIL
                     TVFLVGMMIGRTPEFLGKKVEAREMKYVMIAVIAHPLSILGFTALACLLPATVNSLAN
                     MGPHGFSEVLYAYTSGTANNGSAFAGFNANTPFFNTTIGFAMLVGRYFTLLPMLAVAG
                     VMATKKTVPEGPGTLSTATPLFTGLLVFIALVVGGLTFLPALALGPIVEQLLMLSGTT
                     F"
     gene            complement(3226..3321)
                     /gene="kdpF"
                     /locus_tag="VGM46_13840"
     CDS             complement(3226..3321)
                     /gene="kdpF"
                     /locus_tag="VGM46_13840"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HEY4193723.1"
                     /translation="MTASLITWLAGVCAIGLFAYLFYALVKPERF"
     gene            complement(3828..3904)
                     /locus_tag="VGM46_13845"
     tRNA            complement(3828..3904)
                     /locus_tag="VGM46_13845"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3868..3870),aa:Arg,seq:ccg)
     gene            4062..>4397
                     /locus_tag="VGM46_13850"
     CDS             4062..>4397
                     /locus_tag="VGM46_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY4193724.1"
                     /translation="MPHLKTIGFDADDTLWQNEQFFRLTQQRFTAMLAAHADEETISN
                     ALLDAERRNLAAYGFGIKGFTLSMIETAIEVTKGNVPASTIAEILAAGREMLRHPVVT
                     LPHVGETLHQ"
BASE COUNT          892 a         1301 c         1408 g          796 t
ORIGIN      
        1 ctcgttgggc gtcttgccac gcttggcccc ttcgaccatg gcgatcatgc ggtcgaggaa
       61 ggcttcgccc ggcctggccg taacgcgcat gatgatccag tcggacaaaa cgcgcgtgcc
      121 gccggtgacc gagcagaagt caccgccgga ttcgcggata accggcgcgg actcgccggt
      181 tatggcggat tcgtcgaccg acgccgcccc ctgtatcacc tcgccatcgg cgggtatgat
      241 ctcgccggct tccaccagca cataggctcc gacatcgagt tcgtcgcttg gctgcggcca
      301 ggattcgctt tcgcgggtcg gctccttcag catccgcgca atcacccgtt ggcgcgtggc
      361 ccgcaaggca tccgcctgcg ccttgccgcg gccctcggcc agcgcctcgg cggcattagc
      421 gaaaagcacg gtgaaccaca gccaggcggc gatggcgaag atgaacgcag ccggagcctc
      481 gcccttgccg gtcagcgcct ggatccagag cagcgtggtg atgatgctgc cgagatagac
      541 gacgaacatc accggattct tgacctgaac ccttggcgac agcttcttga acgcctcgcc
      601 gatgaccgag gcccaggaga cgggcttcag ggcgccgccc atatatgcgc cctgcggcgc
      661 ggttgtctct agagaggaca tgatgacacc tcagctattg cgcggcgggg cgagccggaa
      721 cggctgcgtc gagcgcgaga ttgagttcaa gaacgttgat ccgtggatcg ctgaggacgc
      781 cgaactggcg gccggtggtg ttctgatcca gcagttgcat caccttctcg gccgtcagcc
      841 cgcgcgcccc ggcgacacgc gcagcctgaa ggcgggcgtt ggcgaccgag atgtccggat
      901 cgaggccgga ggccgaagcc gtgacggcat cgaccggaac ggaggcgtct gccgccagtc
      961 cattctcgtc tcgataagct ttggcacgtt gggcgaccgc gtcgagcagc ttcttgctga
     1021 tcgcgccctg gttgctcgca gcactcagcg ctgcgttgta aggctggtca accgtctttg
     1081 acggatcgtt cgggtccgtc ccgggcgtga cgcttggacg gccgtggaaa tattccggcc
     1141 tgctgaaata ctggccgatg tggtgcgagc cgaccgcctt gccgtccttg acgatcaggg
     1201 tgcctttcgc ctgatcgggg aacagcaggc tggcgacgcc tgtagtcgcc agcggatagc
     1261 cgataccggt caccagcatg aagaaaaccg cggaggccag caccggccgg gcgacgccct
     1321 tgaacgcctt ctgcggcgcg gcggtatgga tgatggtttg ttcgttgcgc atgaaagtcg
     1381 atccttcaaa atgtcgtgcc ggaaagcatc agcagctgtt cgacgatcgg gccgagcgcc
     1441 agcgccggca ggaaggtcaa tccgccgacg accagcgcaa tgaacaccag gaggccggtg
     1501 aacagcggcg tggccgtcga gagcgtgccg gggccttcgg gcaccgtctt cttggtcgcc
     1561 atcaccccgg ctacggccag catcggcagc agggtgaagt accggccgac gagcatggca
     1621 aagcctatcg tcgtgttgaa gaacggcgtg ttggcgttga agccggcaaa agccgagcca
     1681 ttattggccg tgcccgaagt gtaggcgtaa agcacctcgc tgaagccgtg gggtcccata
     1741 ttggcaaggc tgttgacggt ggccggcagc aggcaggcga gggcggtgaa gccaagtatg
     1801 ctgagcggat gcgcgatcac cgcgatcatc acatatttca tctcgcgcgc ttcgaccttc
     1861 ttgccgagga attccggcgt tcgcccgatc atcatgccga ccagaaacac cgtcaggatg
     1921 gcgtaggtca cgaggttgat gaggccgacg ccatcgccgc cgaacacgtt gttcagcatc
     1981 atctgcgcga tcgtgacgaa cccgccgaga ggcgtcagcg acgaatgcat ggcatcgacc
     2041 gaaccggtcg tcgccgccgt cgtcgtcgtc acgaacatgc tggtctgggc gatgccgaag
     2101 cgaagctcct tgccttccat gttgccaccg gcctgcaagg cggtctgcgc ctggtcgacg
     2161 cccatcgagg ccagcaacgg attgccggcg tgttcggcgc tgtagaccag caccaggaag
     2221 ccgatgaaca tcacgagaaa ggcgccgaaa aacgcccagc cctgacgctg acgcaagacc
     2281 atcgcgccga aagtgtaggt gagcgccgac gggatcaaca gcatgcctag aatatgcaac
     2341 gtgttggtaa gcggcgttgg attctcgaat gggtgcgcgg cattcatgcc gtagaagccg
     2401 ccgccattgg tgccgatgtg cttgatggct tcgagactgg ccaccggccc caggatcaac
     2461 tgttgcctcg ctccctccag ggtggtgacg atcgcgtcgg cgttcagcgt ttgcgggatg
     2521 ccctgccaga catagatgag ggccatgacg aagcagagcg gcaggagcac gcgataaaga
     2581 acgcgcgtga aatcgaccca gtaattaccg atatcggcgg tcgtcttgcg gctgagccct
     2641 cggataaaac cgccggccgc cgcaacgccc gtggtggcgc tgatcgacat caaaaaggtg
     2701 atggcggcca tctgcgagaa gttcgaaagg ctcgcctcgc cggaataggc ctgccaattc
     2761 gtgttggtgg tgaaggaaac ggcggtgttg aaagcgagat ctggggtctg tgcggcgcgg
     2821 gcaagcgtat cgcccggcag gaagccctgt atccgcagga taaaatagcc gagcagcagc
     2881 atggccgcat tgctgagcac cagcacgatg ccgtagcgct tccacgacat tttctcaccg
     2941 ggatcgatgc caaggatgcg gtaggtgccg cgctcgatga aattgtgcgc gggactgatg
     3001 aaaacgtgcg tcatccactt gccgagaaca acggccaagc cggtcatgat cgcaaggatg
     3061 aggacgaatt gaaggatgtc ttgtgccatg tgagcgctct caaattcgat ccagggccat
     3121 gatgaaccct gctgtgattg cgaaggagac aagcgtcacc gctacgaata agaggtcgcc
     3181 cataattgcc accagatgtt tattgttgcg atgtctgttc gatgctcaga agcgctcggg
     3241 cttcacgagg gcgtagaaca ggtaagcgaa gagaccgatg gcgcagacgc ctgccagcca
     3301 ggttatgagc gaagcggtca tggccgcgcc cccacaaacc tgcgactggt cgaatgctgc
     3361 gccgaaccgg cgccatggcg gggaacgaac gcgaacggcg acaagcgcgg cgttggctta
     3421 aatgaaggat gatcgtgcga catgcatacc ggctcctgtg tgaggatcgc cggcaagcat
     3481 ttaacgcctt gcatgtaagg gtagtgttga gtttttacac gcccgcataa gggcggtgta
     3541 aagatcgcag ctaaagccat gcgcctcggt tcggtcatcc aaggacagca cccaatccgc
     3601 ccatcggtct cgcatgccga cgcctgccat aatgattggg acatcgggcg gtgggtctgt
     3661 gatgccgatc ggttcggcgc tccatctcta actcttggta gttgggaagt gaaaagcgcg
     3721 ttgttgccgg catgggtagc cggaaagcct tattcgcagc gtgctacccc ggtgctatcc
     3781 ggagcatttc aggggtcggc aactaatccc caaccctttg aaaaaaatgg cgcacccgac
     3841 aggattcgaa cctgtgacct ctgccttcgg agggcagcgc tctatccagc tgagctacgg
     3901 gtgccttcag gatcagggtc gacccggaaa acgaaccggc caaagatcgg ccagccagcg
     3961 cttcttagcg gaagccggcc aaggcttcaa cgggataaat ggagttgcag gcagcgcggt
     4021 tccggggtgt aaagtggccc catgatgcag gatcaccaga aatgccgcac ctgaagacga
     4081 ttggcttcga tgcggacgac acgctttggc aaaacgagca gttcttccgg ctgacgcagc
     4141 agcggttcac cgccatgctt gccgcccatg ccgacgagga aacgatctcg aacgcgctgc
     4201 tggatgccga acggcgcaac cttgccgcct atgggttcgg tatcaagggg ttcaccctgt
     4261 cgatgatcga aaccgccatc gaagtgacca agggcaatgt tcccgcatca accattgccg
     4321 agattcttgc cgccggccgc gagatgttgc gtcacccggt ggtgacgttg ccgcatgtcg
     4381 gcgagacgct gcaccag
//
LOCUS       DASYCS010000450         1877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_424062,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000450 DASYCS010000000
VERSION     DASYCS010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424062"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..509)
                     /locus_tag="VGM46_13855"
     CDS             complement(93..509)
                     /locus_tag="VGM46_13855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193725.1"
                     /translation="MIRKILGIVAGIVAAYLVILAAEAINHWLYPLPADLDPSDDVAM
                     SAYTFALPLPVRLMYVGGWFAGTFAGAWLNLRIVDWRWGGWIIALLVIADGVARNFEF
                     AEPLWMQAAAVLAPLIGGWLAAKLHRKPYPGEPLLG"
     gene            complement(690..923)
                     /locus_tag="VGM46_13860"
     CDS             complement(690..923)
                     /locus_tag="VGM46_13860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193726.1"
                     /translation="MEPLFYVMAIMGCGDGSDQCAQARVEPVQYRSIRACQAAMPAAL
                     QRNSDLSYPVISAACRTKGQLLTQRQDSQRHSG"
     gene            1024..>1877
                     /locus_tag="VGM46_13865"
     CDS             1024..>1877
                     /locus_tag="VGM46_13865"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEY4193727.1"
                     /translation="MPPMTDTPSADRPLLPTLRRFLPYLWPAGEPALKARIVVAMLLV
                     VASKVVQVYVGPYAYKYAIDHMAHGDRGLGWLIVGLVVAYVAARFGTTLFDNVRNVVF
                     ERVGQEATRRLASRVFRHLHQLSLRFHLERRTGAVTKVVERGTKSIDTMLYFMLFNIA
                     PTILELGMVLQLFGKGFGLWLVASTVVMVALYIVFTRIVTDRRAALRTRMNDLDTGAV
                     AHAVDSLLNFETVKYFGAEEREGRRYEAAMIAYANAATRSENSLAWLNIGQSLITNVM
                     LAFAMGMVA"
BASE COUNT          319 a          613 c          608 g          337 t
ORIGIN      
        1 atgtcggcgc cgatgagctg gaggcattgc tgcgcgacca gatccgctgg gcgctgccga
       61 tgcagaccga tatcgaccac cccgaacccg aattaaccga gtaagggctc gccgggatag
      121 ggcttgcggt gcagcttcgc cgccagccag ccgccgatca gcggcgccag cacggcggcg
      181 gcctgcatcc ataatggctc ggcgaattcg aaattgcgcg ccacgccatc ggcgatgacc
      241 agcagcgcga tgatccaccc gccccagcgc cagtcgacga tccgcagatt tagccatgcg
      301 ccggcgaatg taccggcgaa ccagccgccg acatacatca gccgcaccgg caggggcaaa
      361 gcgaaggtat aggcgctcat cgcgacatcg tcggacggat cgagatcggc gggcagcggg
      421 tagagccagt gattgatcgc ctcggcggcg aggatgacga gataggccgc gacgatgccg
      481 gccacgatac ccagaatctt gcggatcatc gcgcccccga tcgccttgcg ggcgccgtct
      541 tagcgggagt tatgtgcccg gcctagagcg tgctgcgaat aggtggaatc cggctattcg
      601 caaacaagca cgcgaccaca aggaatctag ggcgggctgc gcttccatcg aaacacagcc
      661 tggcctagcg ccctgtgccg gtcgcgggtc tagccgctgt ggcgctggct gtcctgccgc
      721 tgcgtcagca actggccttt tgtacggcac gccgcgctga tcacaggata ggacaggtcg
      781 ctgttgcgct ggagcgccgc cggcatcgcc gcctggcagg cgcggatcga gcgatattgc
      841 accggctcga cgcgcgcctg ggcgcattgg tcggagccgt cgccgcagcc catgatcgcc
      901 atgacgtaga agagcggttc catgccctgt ctcccgtttc ctggagacta aaagcgatga
      961 ggcgcccgac ttgttccacg gggcgcttcc cgatgggctt gaccgtgcct acattgcgtt
     1021 tcgatgccgc cgatgaccga tacgcccagc gccgaccgcc ccctgctgcc gacgctgcgg
     1081 cgcttcctgc cctatttgtg gccggccggt gagccggcgc tcaaggcgcg catcgtcgtc
     1141 gcgatgctgc tcgtcgtcgc gtccaaggtg gtgcaggtct atgtcgggcc ctatgcctac
     1201 aaatacgcca tcgaccacat ggcgcatggc gaccgcggcc ttggctggct gatcgtcggc
     1261 ctcgtcgtcg cctatgtcgc ggcgcgcttc ggcacgaccc tcttcgacaa tgtccgtaat
     1321 gtcgtcttcg agcgcgtcgg ccaggaagcg acgcggcggc tggcatcgcg cgtgttccgc
     1381 catctccacc aattgtccct gcgctttcat ctcgagcggc gcaccggcgc ggtcaccaag
     1441 gtggtcgagc gcggcaccaa gagcatcgac acgatgctct acttcatgtt gttcaacatc
     1501 gcgccgacca tcctcgagct gggcatggta ctgcagcttt tcgggaaggg gttcggcctg
     1561 tggctggtcg cctcgaccgt ggtgatggtg gcgttgtaca tcgttttcac gcgcatcgtg
     1621 acggaccggc gcgcggcgct gcgcacgcgg atgaacgacc tcgacaccgg cgcggtggcg
     1681 catgcggtgg attcgctgct caatttcgaa acggtcaaat atttcggcgc cgaggagcgc
     1741 gaggggcggc gttacgaggc agcgatgatc gcctatgcca acgccgcgac gcgcagcgag
     1801 aattcgctcg cctggctcaa tatcggccaa tccctgatca ccaatgtgat gctggccttc
     1861 gccatgggca tggtcgc
//
LOCUS       DASYCS010000451         1922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3325403,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000451 DASYCS010000000
VERSION     DASYCS010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3325403"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VGM46_13870"
     CDS             complement(<1..251)
                     /locus_tag="VGM46_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEY4193728.1"
                     /translation="MRRLFKRLLPKSWRSDIVTIPVVRLHGTIMAGGGKFRPSLSLAS
                     TAGLIEKAFAFDAPAVAISINSPGGSPVQSRLIYQRIRDL"
     gene            complement(322..1107)
                     /locus_tag="VGM46_13875"
     CDS             complement(322..1107)
                     /locus_tag="VGM46_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019169852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEY4193729.1"
                     /translation="MADAASAPAYTVDAFHRGRFFLVQPAASGHRAGMDAMMLAAAVP
                     SSFTGRMADFGAGAGAAGLAVLSRCSNAQAVLVERAAEMAAFADTTLGHPGNAHLGDR
                     ACVLRADVTLSGRARQTAGLADRSFDFVVMNPPFNAAQDRATPDELKRQAHVMEDGLF
                     EQWLRSAAAVLKPRGGLALIARPEQLSVILDALSGRFGNAELMAVHPKSDAAAIRIIV
                     RAIRGARGKLSICPPLVMHEGAGNAFSARAELIGNGLASLFGD"
     gene            complement(1111..1335)
                     /locus_tag="VGM46_13880"
     CDS             complement(1111..1335)
                     /locus_tag="VGM46_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006330294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEY4193730.1"
                     /translation="MIELIRTNDVVVISFVESLMRDAGIGCFVADQNMSVMDGSLGIL
                     AKRVMVDADRADEARRIVTDAGMGGEIRGN"
     gene            1495..>1922
                     /locus_tag="VGM46_13885"
     CDS             1495..>1922
                     /locus_tag="VGM46_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010658340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEY4193731.1"
                     /translation="MGVVLNLDNGKREAATIKELIALTATDMGRVNELILSRAGSDVE
                     MIPEIANHLISSGGKRLRPMVTLAAAQMFGYAGEGHVKLATSVEFMHTATLLHDDVVD
                     ESGMRRGKKTARMIWGNQASVLVGDFLLGQAFRMMVDVGSL"
BASE COUNT          342 a          631 c          632 g          317 t
ORIGIN      
        1 aggtcgcgaa tgcgctggta gatcagccgc gattgcaccg gcgagccgcc cggcgagttg
       61 atcgagatgg cgacggccgg cgcgtcgaag gcaaacgcct tttcgatcag gccggcggtc
      121 gaagccagcg acaggctcgg ccggaacttt ccgcctccgg ccatgatggt tccatgcagt
      181 cgcaccaccg ggatggttac gatgtcggag cgccaggatt taggcagcag gcgtttgaaa
      241 agtcggcgca agaggcggtt ctccttcggg ggcctgaagc atgtaggcat tcgccggcga
      301 acggcaatgc cgatgggcgg ttcaatcgcc gaaaagcgac gccagcccat tgccgatcaa
      361 ttcggcgcgg gccgaaaatg cgttgcccgc tccctcatgc atcaccagcg gcgggcagat
      421 ggacagtttt ccgcgcgcgc cgcgaatggc ccggacgatg atgcggatgg ccgccgcatc
      481 ggatttggga tgcaccgcca taagctcggc attgccgaac cggccggaaa gcgcgtcgag
      541 gatgacgctc aattgttcag gccgggcgat cagcgccagc ccgccccgcg gcttcagcac
      601 ggcggctgca cttcgcagcc actgttcgaa cagcccgtct tccatcacat gcgcctgcct
      661 cttcaattcg tccggcgtgg cgcggtcctg cgccgcgttg aacggcgggt tcatgacgac
      721 gaaatcgaag ctccggtcgg caaggccggc ggtctggcgg gcgcggcccg aaagcgtcac
      781 gtcggcccgc agcacgcagg cgcggtcgcc gagatgggca ttgcccggat ggccaagcgt
      841 ggtgtcggca aacgccgcca tctcggccgc gcgctcgacc agcacggcct gcgcattcga
      901 acagcgcgac agcacggcaa ggcctgccgc gccggcgccc gcgccgaaat ccgccatccg
      961 gccggtgaag gaagaaggca ccgccgccgc cagcatcatc gcatccatgc cggcgcggtg
     1021 tccgcttgcc gccggctgca ccaggaagaa acgtccgcga tggaacgcat cgaccgtata
     1081 ggccggcgct gatgcggcgt cggccacggc ctagttcccc cggatctcgc cgcccatgcc
     1141 ggcatcggtg acgatgcggc gcgcttcgtc ggcgcggtcg gcatccacca tgacgcgctt
     1201 ggccagtatg ccgagcgagc cgtccatcac gctcatgttc tggtccgcga cgaaacagcc
     1261 gatgccggcg tcgcgcatca gcgattcgac gaaggagatg acgacaacgt cattggtgcg
     1321 aatgagttcg atcacttggg tcactccggt ctgccaggct catgtaaccg cacgcggcgc
     1381 gggacgccat aggcccccgc gccatttcgc ctcttgccgt ggctaggggt gccgccctac
     1441 tgtctggtca acgaaaggtg ccggcgtcgc gcatttttga cgggagtgtt tgtcttgggt
     1501 gtcgttctca atctcgacaa cggaaagcgg gaagccgcga ccatcaagga actgatcgcg
     1561 ctgacggcga ccgacatggg ccgcgtcaac gaactgatcc tttccagggc cggctccgat
     1621 gtcgagatga tcccggaaat cgccaaccac ctgatttcgt cgggcggcaa gcgcctgcgc
     1681 cccatggtga cgctggctgc cgcccagatg ttcggctatg ccggcgaagg ccatgtaaag
     1741 ctcgccacca gcgtcgagtt catgcacacc gcaacgcttc tccacgacga tgtcgtcgat
     1801 gaaagcggca tgcggcgcgg caagaagacg gcgcgcatga tctggggcaa ccaggccagc
     1861 gtgctggtcg gcgacttcct gctcggccag gctttccgca tgatggtcga tgtcggctcg
     1921 ct
//
LOCUS       DASYCS010000452         6630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4630746,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000452 DASYCS010000000
VERSION     DASYCS010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 6630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6630
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4630746"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="VGM46_13890"
     CDS             <1..724
                     /locus_tag="VGM46_13890"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEY4193732.1"
                     /translation="QVMREAIVQSAILSAEMAEEIGLAREKIILSAKVSGVQDLIAVY
                     AELATRSNHALHLGLTEAGMGSKGIVASSAAMGILLQQGIGDTIRISLTPEPNGDRTR
                     EVQVAQELLQTMGFRQFVPIVAACPGCGRTTSTVFQELAQSIQADLRKNMPVWREKYP
                     GVENLKVAVMGCIVNGPGESKHADIGISLPGTGEIPTAPVFVDGKKAATLRGPDIAAE
                     FEKMVAAYIEQRFGHGSQAAAE"
     gene            738..1583
                     /locus_tag="VGM46_13895"
     CDS             738..1583
                     /locus_tag="VGM46_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY4193733.1"
                     /translation="MRAAIAAALLALAWANAALADPPPTARETLVGRVCDLIETHALE
                     NGLPKEFFARLIWKESRFDPNAVSPVGAEGIAQFMPGTAKMRGLANAFDIEQAIPASA
                     RYLGELKTGFGNLGLAAAAYNAGESRVSRWLSSGGFLPLETEEYVLDVMGEPADNFTD
                     RSHAGIVRPLDKKISFSSACRILPTIMSKTIAMASVNIKPWGVQVAGNFRRAAAVRQW
                     QRVQGRFHALLGSYEPVVSRVRTPIGRRGVYAVRIGVNDRASASDICQKLRGAGGACI
                     VKRNR"
     gene            complement(1718..4054)
                     /locus_tag="VGM46_13900"
     CDS             complement(1718..4054)
                     /locus_tag="VGM46_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY4193734.1"
                     /translation="MPEYSAFIRSIRIRYISGLLILALSVGAGIIMLNRAGSYRHQVD
                     LLGGHMVKFAGDLNEASNFAESASASWRPDKREELAKSAKERAGRLTADIQTLSDQLL
                     DISPHLDKSTTRDLTAASVNGYLLWSAREIVRNLDLIASAADAGPPGAHDIANQNNLF
                     AQPMLIRARTAMDNERRLADAHSDRLLAFGTVLLALALAIVAIWIFRPMEMAIRRAFT
                     ETTRSLIKAEAADRAKSEFLANMSHEIRTPMNGVLGMAELLAKTELSPRQKTFTDVIV
                     KSGNALLTIINDILDFSKINAGQLTLDPGPFRLIEAVEDVATLVSARVAEKNLELIVR
                     VDPHLPAFVVGDAGRFRQIITNLLGNAVKFTEKGHVLIDVGGEIVDGVARMKVRVEDT
                     GIGIPAEKIQSVFEKFAQVDSSSTRRHEGTGLGLAISARLVDLMGGKIGVESEIGRGS
                     VFWFSASMPVHKEQARDESVPVDVTGARVLVIDDNPVNREILLEQLRSWGFDCAAAES
                     GAVGLAFLHRASQLGARVDCVILDYQMPGMTGADVAKAISADSRLSAIPVVLLTSVDQ
                     VDFGRLILDYGIAAHLTKPARSAVLLGTVISSIQKARRQIGKAHFVRDSAAAGPAVAH
                     PAFTVIRSAPTPQATMPDAPAANGPIDILIAEDNEINQLVFSQILTGLGLNYRIAGNG
                     RTVVEMYRALKPRLILMDVSMPEMNGFEATRAIRADEAASGERTPIIGVTAHALKGDR
                     DKCMEAGMDDYLPKPVSPDRLGAKIGTWLSEREIAKSA"
     gene            complement(4230..5147)
                     /locus_tag="VGM46_13905"
     CDS             complement(4230..5147)
                     /locus_tag="VGM46_13905"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018430051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEY4193735.1"
                     /translation="MEIGGDMVLEAALGLRAEAVEAVLRRLLDDRALSGEIARPAGLM
                     AAMRHGVLNGGKRLRPFLVLESAALFSAGGEAALRVAAALECVHCYSLIHDDLPAMDD
                     DDLRRGQPTVHKAFDEATAILAGDALLTLAFDIIADEATVIAPERRIALVLALARAAG
                     VGGMAGGQMLDLRAATVRPDEAGIITLQAMKTGALIRFACEAGAIIAGSPADDRERLA
                     EFGSAIGLAFQLADDLLDLTADAGQMGKATGKDEAAGKATLVALHGVDWARSQLRGLV
                     GQAHMLLEPYGESAAMLKAAAAFVAERRN"
     gene            5274..5984
                     /locus_tag="VGM46_13910"
     CDS             5274..5984
                     /locus_tag="VGM46_13910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEY4193736.1"
                     /translation="MAARPMRWNRTGAKRWMRRGIVAALVLAAIPAVLTILYLPYFVH
                     PVSTLMLKDLATFSGYDRRWVSIDDVAPVLAHSVIMSEDGQFCFHRGIDLAELKGVVG
                     DAMQGEATRGASTITMQTVKNLFLWSRPMASARKLVELPLAVYFDAVMSKRRIMEIYL
                     NVAEWGPGIYGIEAAAQHHFGVSANRLTRRQAALLAVTLPNPIARNPSKPGHGLRRLA
                     GLIERRAARSGAYVGCLN"
     gene            6131..6313
                     /gene="rpmF"
                     /locus_tag="VGM46_13915"
     CDS             6131..6313
                     /gene="rpmF"
                     /locus_tag="VGM46_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEY4193737.1"
                     /translation="MAVPKRKTSPSKRGMRRSADALKNPTYVEDKNSGEMRRPHHIDL
                     KTGMYRGRQVLEPKES"
     gene            complement(6471..>6630)
                     /locus_tag="VGM46_13920"
     CDS             complement(6471..>6630)
                     /locus_tag="VGM46_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEY4193738.1"
                     /translation="DGRVVPVAGGVLIRDEAGEIIGAVGISGDTSDNDEAAAIAGIEA
                     AGFTPDAG"
BASE COUNT         1197 a         2177 c         2097 g         1159 t
ORIGIN      
        1 gcaggtgatg cgcgaagcca tcgtgcagtc ggccatcctc tccgccgaaa tggccgagga
       61 aatcgggctg gcgcgggaaa agataatcct gtcggccaag gtcagcggcg tgcaggactt
      121 gatcgccgtc tatgccgagc tggcgacgcg ctcgaaccat gcgctgcatc tgggcctgac
      181 ggaagccggc atgggatcga agggcattgt cgcctcgtcc gcagccatgg gcatcctgct
      241 ccagcagggc atcggcgaca ccatccgcat ctcgctgacg ccggagccga acggcgaccg
      301 cacgcgcgag gtgcaggtgg cgcaggagct gctccagacc atggggttcc gccaattcgt
      361 gcccatcgtc gccgcctgcc ccggctgcgg ccgcaccact tccaccgtgt tccaggaact
      421 ggcgcagtcg atccaggccg acctgcgcaa gaacatgcct gtctggcgcg agaaatatcc
      481 gggcgtcgaa aacctcaagg tcgcggtcat gggatgcatc gtcaacggac cgggcgagtc
      541 caaacatgcc gatatcggca tttccctgcc cggcactggc gagataccga ctgcgccggt
      601 gttcgttgac ggcaagaagg ccgctacctt gcgtggcccc gacatcgccg ccgaattcga
      661 gaagatggtt gccgcctata tcgagcaacg cttcgggcat ggcagccaag ccgccgccga
      721 ataggcctgt ttggacaatg cgcgcggcaa tcgccgcagc attgttggct ttggcttggg
      781 caaacgcggc gctggccgac ccgccgccaa ccgcccgcga gacgctggtt ggccgcgtgt
      841 gtgacctgat cgaaacccat gcgctggaaa acggcctgcc gaaggaattc ttcgcccggc
      901 tgatctggaa ggaaagccgc ttcgatccca atgcggtcag cccagtcggg gccgagggca
      961 tcgcccagtt catgcccggc accgccaaaa tgcgcggcct tgccaacgcg ttcgacatcg
     1021 aacaggccat tcccgcctcc gcccgctatc tcggcgaact gaaaaccggc ttcggcaatc
     1081 tcggccttgc cgccgccgcc tataatgccg gcgaaagccg cgtctcgcgc tggctttcca
     1141 gcggcggttt cctgccgctc gaaaccgagg aatacgtgct cgacgtgatg ggcgagccgg
     1201 ctgacaattt caccgaccgc agccatgccg gcatcgtccg ccccctcgac aagaagatca
     1261 gcttttcatc cgcctgccgc atcctgccga cgatcatgtc gaagaccatc gccatggcgt
     1321 cggtcaacat caagccatgg ggcgtacagg tggccggcaa tttccgccgc gccgccgctg
     1381 tccggcaatg gcaaagggtg cagggccgct tccatgcgct gctcggcagt tacgagccgg
     1441 tggtcagccg cgtgcgcacg ccgatcggcc ggcgcggcgt ctatgccgtg cgcatcggcg
     1501 tgaacgaccg cgcaagcgcc agcgacatat gccagaagct gcgcggcgcc ggcggcgcct
     1561 gcatcgtcaa gcgcaaccgc tagggcgata gagcatttcc agtaaaagtg tgaaacggtc
     1621 ttgcgtccgg aaatgcggca aaaacaaaga gatagagcgg ttttaccaat tcggtaaaaa
     1681 ccagaaccgc tctagcggcg cgccaactgc cttggcgtca agccgatttg gcaatttccc
     1741 gttcgctgag ccaggtgccg atcttggcgc cgagacggtc gggcgagacg ggcttgggca
     1801 gatagtcgtc catgccggcc tccatgcatt tgtcgcggtc tcccttcagc gcatgcgcgg
     1861 tgacgccgat gatcggcgtt cgctcgccgc tggccgcctc gtcggcacgg atggcccgcg
     1921 ttgcctcaaa gccgttcatt tcgggcatcg agacatccat caggatcagc ctcggcttga
     1981 gcgcccggta catctccacc accgtgcgac cattgccggc gatacggtaa ttaagcccca
     2041 gcccggtgag tatctggctg aagaccagtt ggttgatctc attgtcctcg gcaatcagga
     2101 tatcgatcgg cccgttggcc gcaggcgcat ccggcatggt tgcctgcggt gtcggcgcag
     2161 agcggatgac cgtgaaggcg ggatgggcga cggccgggcc ggcggcagcc gagtcgcgca
     2221 cgaaatgcgc cttgccgatc tgcctgcgcg ccttttgtat ggatgaaatg acggtgccca
     2281 gcagcacggc cgagcgcgcc ggcttggtaa gatgcgccgc aatgccgtag tcgaggatca
     2341 gccgtccgaa atccacctgg tcgacggagg tcagcagcac caccggaatg gccgacagcc
     2401 ggctgtcggc ggagatcgcc ttggcgacat cggcgccggt catgccgggc atctggtagt
     2461 cgaggatgac gcaatcgacc cgcgcgccaa gctgggaggc gcggtgaagg aaggccagcc
     2521 ccaccgcacc gctttccgcc gccgcgcaat cgaaacccca gctgcgcaat tgttcgagca
     2581 ggatttcgcg attgacggga ttgtcgtcga tcaccagcac ccgcgcgccc gtcacgtcga
     2641 ccggcacgct ttcgtcgcgc gcctgttcct tgtggaccgg catcgaggcg gagaaccaga
     2701 ataccgagcc gcgcccgatc tcgctttcca cgccgatctt gccgcccata agatcgacaa
     2761 gccttgcgct gatggcgagg ccgaggcccg tgccttcatg gcggcgcgtc gaggaggaat
     2821 ccacctgcgc gaacttctcg aacacgctct ggatcttttc agccggaatg ccgatgccgg
     2881 tatcctcgac cctcaccttc atccgcgcca cgccgtccac gatttcgccg ccgacatcga
     2941 tcagcacatg gcctttctcg gtgaatttca ccgcattgcc gagcagattg gtgatgatct
     3001 ggcggaagcg gccggcatcg ccgacgacaa aggccggcag atgcggatcg acgcgaacga
     3061 taagctcaag gttcttttcg gcgacgcgcg ccgaaaccag cgtcgccacg tcctccaccg
     3121 cctcgatcag tcggaacgga cccggatcga gcgtaagctg cccggcattg atcttcgaaa
     3181 aatccagaat atcgttgatg atcgtcaaaa gcgcgttgcc ggatttcacg atgacgtcgg
     3241 tgaaggtctt ctggcgcggc gaaagctcgg tcttggccag cagttcggcc atgccgagga
     3301 cgccgttcat cggcgtgcga atttcatggc tcatattggc caggaattcc gacttggcgc
     3361 ggtccgccgc ctccgccttg atcagcgatc tggtggtttc ggtgaaggcg cggcggatcg
     3421 ccatttccat cggacggaat atccatatcg ccacgattgc cagcgccagc gccaaaagga
     3481 cggttccgaa ggccagcagc ctgtcgctat gggcgtcggc gaggcggcgc tcattgtcca
     3541 tcgccgttcg cgcgcgaatg agcatcggct gcgcaaacag gttgttctgg ttggcgatgt
     3601 catgcgcacc cggcgggccg gcatcggcgg ccgaggcgat caggtcgaga ttgcgcacga
     3661 tctcgcgcgc cgaccacagc agatagccgt tgacggaggc cgccgtcagg tctcgcgtcg
     3721 tgctcttgtc cagatgcggg ctgatgtcca gcaactggtc ggacaaggtc tggatgtcgg
     3781 ccgtcaggcg gccggcacgc tccttggcgg atttcgccag ttcctcacgc ttgtccggcc
     3841 gccaggaagc ggaagcggat tcggcaaaat tggaagcctc gttgaggtcg ccggcaaact
     3901 tcaccatatg gccgccaagc aggtcgactt gatggcggta ggaacctgcc cggttgagca
     3961 tgatgatgcc cgcgcccacc gacagcgcaa ggatcagcag cccggatata tagcgtatcc
     4021 ggatggacct gatgaaggcg gaatattccg gcatgcgcga ccccagacca aacacgctat
     4081 ttagcggccg cgattacgct ctattatcgt taaaattccg ttctggcggt tcggccgacg
     4141 gctgttccag ccgtcaattg cctgaccgtc tgctcgtccg gccgtcagtt gcctggccgt
     4201 tacttggccg accgccactt gggcgaccgt cagttgcgcc gctcggcgac gaatgcggcc
     4261 gctgccttca gcatggcggc gctttcgcca tatggctcaa gcagcatgtg cgcctggccg
     4321 accaggccgc gcagctggct ccttgcccag tcgacgccgt gcaacgccac cagcgtcgcc
     4381 ttgccggcgg cttcatcctt gccggtcgcc ttgcccatct ggccggcatc agcggtaagg
     4441 tcgagcaggt catcggccag ttgaaaggca aggccgatgg ccgagccgaa ttcggcgagc
     4501 ctctcgcggt cgtcggcggg cgagccggcg atgatcgcgc ccgcctcgca cgcgaagcgg
     4561 atcagcgctc ccgtcttcat cgcctgtagc gtgatgatgc cggcctcgtc cgggcgaacg
     4621 gtcgcggcgc gaagatcgag catctgcccg ccggccattc cgccgacgcc ggcggcgcgc
     4681 gcaagcgcca gcaccagcgc tatcctgcgt tccggcgcga tgacggtggc ttcatcggcg
     4741 atgatgtcga aggcgagcgt cagcaatgca tcgccggcca gaatggcggt ggcctcatcg
     4801 aaggccttgt gcacggtcgg ttggccacgg cgcaggtcgt cgtcgtccat cgccggcagg
     4861 tcgtcgtgga tcagcgaata gcagtgaacg cattccagcg cggcggccac gcgcaaggca
     4921 gcttcgccgc ctgcgctaaa cagcgccgca ctttccagca ccagaaacgg gcgcaggcgc
     4981 ttgccgccgt tcaacacgcc atggcgcatc gccgccatca ggccggcggg ccgggcgatt
     5041 tcgccggaaa gcgcgcggtc gtcgagcaat cgccgcagca ccgcctcgac cgcctcggcc
     5101 cgcaggccga gcgccgcttc caacaccatg tcgccgccga tctccatggc ggcgaccggt
     5161 agccgacaca atggcgttgc gcaagaagcc ttccgccatt atgccgttcg ggggaccaca
     5221 ggcggggtga cgtgtcggaa ccgatcatcg atccggaaac cgaaaggctg ggaatggcgg
     5281 cgcggccaat gaggtggaac cgcaccgggg cgaagcgttg gatgcggcgc ggcatcgtcg
     5341 cggcgctcgt gctcgcggcc ataccggccg tgctcaccat cctctacctg ccatatttcg
     5401 tgcatccggt ttccacgctg atgctgaagg atctggcgac gttttccggc tatgaccgac
     5461 gctgggtttc gatagacgac gttgcgccgg tattggcgca ttcggtcatc atgtccgagg
     5521 acgggcaatt ctgcttccat cgcggcatcg atcttgccga actgaaaggc gtcgtcggtg
     5581 acgcgatgca gggcgaggcg acgcgcggcg cctcgaccat caccatgcag acggtgaaga
     5641 acctgttttt gtggtcgcgg ccgatggcca gcgcgcgcaa actggtcgaa ctgccgctcg
     5701 ccgtctattt cgacgcggtc atgtcgaagc ggcggatcat ggaaatctac ctcaacgtcg
     5761 ccgaatgggg tcccggcatc tacggcatcg aggccgccgc ccagcatcac ttcggcgtct
     5821 ccgcgaaccg gctgacgcgg cggcaggccg ccttgctcgc cgtgaccctg ccgaacccaa
     5881 tcgcgcgcaa tccgtccaag cccggacatg gcctgcgccg gctggcgggc ctcatcgaac
     5941 ggcgcgcggc gcggtcgggc gcctatgtcg gctgcctgaa ttaaaacggc gcaacgaaaa
     6001 agtatgcgcc gctactggcc aagccggcat cgagcgtgta taggcagccg actttctcag
     6061 attccagcct ctgcatgtgg ctctttcacg agggcatgcg gggcttttga ccatgtaatg
     6121 gagcatatcc atggccgttc cgaagcgaaa aacctctccc tccaagcgtg gcatgcgccg
     6181 ttcggccgac gcgctcaaga acccgaccta tgtcgaggac aagaactccg gcgagatgcg
     6241 ccgcccgcac catatcgacc tgaagaccgg catgtatcgc ggccgtcagg ttctggagcc
     6301 gaaggaaagc tgaaaccagc tttcatcggc agatgatctt gtcaaaagac cggccactgg
     6361 ccggtctttt gcatttcggc agggcagtcg caggccacga accggcggcg tgaggcaatg
     6421 cccggcggcg aagccttccg ccgccaatcg ggtattgacc caggctggcc tcaaccggcg
     6481 tcgggcgtga agccggcggc ctcgatgccg gcgatggccg ccgcctcgtc attgtccgac
     6541 gtatcgccgg aaatgccgac ggcgccgatg atttcgccgg cctcgtcgcg gatcagcacg
     6601 ccgcccgcaa ccggcaccac ccgcccatcg
//
LOCUS       DASYCS010000453        11282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2168489,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000453 DASYCS010000000
VERSION     DASYCS010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 11282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11282
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2168489"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..309
                     /locus_tag="VGM46_13925"
     CDS             64..309
                     /locus_tag="VGM46_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2093 domain-containing protein"
                     /protein_id="HEY4193739.1"
                     /translation="MMNRFEGPGGREARIRYLDGDFQVTSPGAFVRCAVTGESISLDE
                     LKYWSVERQEAYATAAISLDRELAAHPEWRTRGSSAA"
     gene            complement(306..1334)
                     /gene="lpxK"
                     /locus_tag="VGM46_13930"
     CDS             complement(306..1334)
                     /gene="lpxK"
                     /locus_tag="VGM46_13930"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEY4193740.1"
                     /translation="MASEAPPFWWQKPDWRAYLLSPLSLAYGAVAGRRMKTAKREKID
                     APVLCIGNFTVGGTGKTPVAIALAQRAKQMGLKPGILSRGHGGSFGEPHVVDAQHDGA
                     RHVGDEPLLLAAHAPVAVTPDRAAGARILLEKHGCDFLIMDDGFQSARIHIDYALVVI
                     DARHGLGNGHVIPGGPLRARISDQLPFTDAVLKMGDGEAADALVRKAARGGKPIFEAR
                     AEPRDKKMFAGKHLLAFAGIGHPDKFFATLVEAGGIVEAARPFPDHHFYAGDELAELA
                     ETAKVAGLELVTTAKDAMRLRHDHAPAGFLDRLSVLEIDAVFEADQIPDRIVEQTLEA
                     WRRRRLQG"
     gene            complement(1339..2655)
                     /gene="waaA"
                     /locus_tag="VGM46_13935"
     CDS             complement(1339..2655)
                     /gene="waaA"
                     /locus_tag="VGM46_13935"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HEY4193741.1"
                     /translation="MSERWARSVLMMYRCAGAAAYPLVGPYVAWRVAKGKEDRSRSRE
                     RYGVTKRERPQGPLIWVHAASVGETIAVVPLVESILDYGVNIVLTTGTITSAKVADER
                     LGDRIIHQYVPLDLKPAVSRFLNHWRPDLAIIAESEIWPMTILELGARHVPQVLVNGR
                     LSDRSFASWKKRSNIAEALFENLAHVVAQSEADGERFKELGARPVSVSGNLKVDTKPP
                     PVDEKTLHGLKRQIDGRPTWAAVSTHEGEEVIAAEVHTTLSARHPGLLTIIVPRHPDR
                     ADALVGQFAEMGLKVARRSAGEKVGPDVDILLGDTIGEMGLYLRLTEIAFVGRSLTSE
                     GGQNPLEPAMLETAVLAGRNVQNFRESYQRLLDRGGAKLVRDRAMLVGAVNFLLTNEA
                     ARRDMMAAGAATVEEMRGALAKTLKALEPYIQPLVLKARLKNASAR"
     gene            complement(2652..3440)
                     /locus_tag="VGM46_13940"
     CDS             complement(2652..3440)
                     /locus_tag="VGM46_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY4193742.1"
                     /translation="MDQRVATSRAVNSMPEKKRSGKTKALWKKVRQPLAKSRAVKGAV
                     ASLIALGLKFVRLTNPLANGSVRILGGQYHDLEPVIVALWHGQHLLAPVFYPKGSGLA
                     AMVSRSADAELNAIVLAKFGIDIVRGSGGREGEHKSDKGGAKALIALKKTLRAGTNVC
                     MIADIPHGTPRDSGLGIVLLARLSGRPILPVAFTTSRRKILEKSWDKTTINLPFGRSS
                     TVVGQPIYVSADADDAEMERKRQEVTEALNAATAQAYRMVDGPR"
     gene            complement(3442..3687)
                     /locus_tag="VGM46_13945"
     CDS             complement(3442..3687)
                     /locus_tag="VGM46_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010661008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4170 domain-containing protein"
                     /protein_id="HEY4193743.1"
                     /translation="MSGDSEKKQLLHLVFGGELKKVGGTEFRDLDALDIVGVYPDYET
                     AKTVWKAKAQASVDNAHMRYFVVHLHRLLDPDGKADA"
     gene            complement(3758..4567)
                     /locus_tag="VGM46_13950"
     CDS             complement(3758..4567)
                     /locus_tag="VGM46_13950"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006332233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HEY4193744.1"
                     /translation="MPAADPTDLPSAASDLALLLDAAREAGVIAMNYFGKDPEVWMKG
                     GTSPVSEADYAADKYLRTTLLAARPDYGWLSEETADDPVRLSARRTFVVDPIDGTRGF
                     LQGLDSWCVCVAVVEGGKTIAGVLECPAKQETYWAMPGEGAFLNGKAISIRRDATPPL
                     EIAGPKALIDLLAPDWQRHLARAPYIPSLAYRLAMVASGVLDATFVKPNAHDWDIAAA
                     DLILREAGGQLVGQDGNSPTYAGEMIRHGALAAGSGALFAAMTEVIAQADF"
     gene            complement(4554..5897)
                     /locus_tag="VGM46_13955"
     CDS             complement(4554..5897)
                     /locus_tag="VGM46_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006204047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEY4193745.1"
                     /translation="MTDAPDAAKLTDRVTALVEAAKRAGADAADAVAVRGRSTGVSVR
                     LGKVEATEASESEDVALRVFVGARVASVSATASSDPKALAERAVAMAKVSPEDIYQGL
                     ADTALLVKSPRDLDLFDGTEVSAERLKQDALAAEEAALAVKGVTNSGGSSASAGLGGL
                     VLATSNGFVGQYVASRFSRSASVIAGEGTGMERDYDFSSRQHFSDLDGPQTIGRSAGE
                     RAVRRLGARKAATGSVDVVFDPRVSRGIAGHLAGAINGASVARKTSFLREMMGKQVAA
                     AAITVTDEPLRRRGQASRPFDGEGIEGEKLIMVEKGFLNHWFLSTSAARELGLTTNGR
                     GSRGGSSVSPSSTNLAIEPGDRTPEDIIGSMKSGFYVTEVFGQGVNMITGEYSRGASG
                     FWIENGALAYPVSEVTIASNLKTMFLNMVPASDLDRNFGTAAPTLLIEGMTLAGS"
     gene            6069..6485
                     /locus_tag="VGM46_13960"
     CDS             6069..6485
                     /locus_tag="VGM46_13960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY4193746.1"
                     /translation="MTPFHLAFPVTDLDETRAFYGEVLGCSIGRFSETWVDFDLFGHQ
                     MSAHLRPQATAAARDGKVDGVVVPIPHFGAVLLMDEWERLAARLVARDDIDWLEKPMV
                     RFKGEPGEQATLFIRDPSGNALEFKGFRSLEQIFAH"
     gene            complement(6547..6906)
                     /locus_tag="VGM46_13965"
     CDS             complement(6547..6906)
                     /locus_tag="VGM46_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018428184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEY4193747.1"
                     /translation="MNRLVSAFVNSCRAFGRLAATEQAFQQELIVLLLAFPAGWFLSS
                     SWQHYALLIGAVLLLIVVEVLNTGIEAVCDAVSREFNADIQLSKDCGSLAVLISVVMA
                     AAVWGLALVEWVLGTAI"
     gene            complement(6988..7407)
                     /locus_tag="VGM46_13970"
     CDS             complement(6988..7407)
                     /locus_tag="VGM46_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY4193748.1"
                     /translation="MSTMRTGSCLCGAVHFRTHGNLRGVIYCHCSQCRKQTGHVVAAT
                     NSKDADIVIEGGEALKWYGASDAAKRGFCGTCGSVMFWKHHDLDSISIMAGSFDRPSG
                     LAGKSHIFTGDKGDYYTIDDGLRQFEKSSPSVKVAQD"
     gene            7700..8770
                     /locus_tag="VGM46_13975"
     CDS             7700..8770
                     /locus_tag="VGM46_13975"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY4193749.1"
                     /translation="MQPALAQTERATDIATTIVATMRQLGVLGLPRNYEIFYEALTGS
                     NSELRLAVVSLGDKPTQEELDRIGHRFFAQNHGHGIVEQARDVIAKELEDIALLLRSE
                     RTHIEKYGKILNETSSGLSNRNVISNDLLQKIVSAMSVATTSTIDHGKHVATTLTEKT
                     AELESVKTKLEEYKRLADTDPLTQIWNRRAFDKEIAKIYNSNKGILFNALILADIDRF
                     KNINDRFGHPVGDKIIQLIAEIFQTGVRGDIFVARTGGEEFALIVEGASEDTTYEIAD
                     RIRALIEQTPFASSSTGASCGSVTVSMGMCMASEADGPEDLYTKADRALYRSKVSGRN
                     QVTRHSSMAGRTSKNWLLYKKD"
     gene            complement(8838..10211)
                     /locus_tag="VGM46_13980"
     CDS             complement(8838..10211)
                     /locus_tag="VGM46_13980"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019858372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HEY4193750.1"
                     /translation="MTKWSPNSWRAKPIQQVPAYPDANALKATEAQLATFPPLVFAGE
                     ARKLKKQLATVAAGDAFLLQGGDCAESFAEHGADNIRDFFRVFLQMSVVLTFAGSQPV
                     VKIGRVAGQFAKPRSSDNETKGDETLPSYRGDIINGIDFDAKSRIPDPSRQVMAYRQS
                     AATLNLLRAFAQGGYASLENVHSWMLGFVSDSPQGEKYESLANRITETMDFMKAVGIT
                     SETNYALRETDFYTSHEALLLGYEEALTRVDSTSGDWYATSGHMIWIGDRTRQPDHAH
                     VEYCRGIKNPLGLKCGPSMTADGLMELIDLLNPENEPGRLTLIARFGADKAGDHLPKL
                     VQAVKKAGRSVVWSCDPMHGNTITAAGYKTRPFDRILKEVQTFFEVHRAEGTHPGGIH
                     IEMTGKNVTECTGGARAITAEELQDRYHTHCDPRLNADQAIELAFLVSDLLKKTQPVL
                     HKRAMTA"
     gene            10801..>11282
                     /locus_tag="VGM46_13985"
     CDS             10801..>11282
                     /locus_tag="VGM46_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006333620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEY4193751.1"
                     /translation="MRGFWGLIRAYWFSDHWKEAWSLTIIIAALTAISSKTGVWFAEM
                     SGELVNSIAFYHDAANTAPLRSLLTNAGMLVVLVIIKDAGITGTKSFVSSTLHRKWRK
                     WLNGRFNEALLDPNHTHFHTQNGSDGSVPDNIDQRVQESIKGVTGGAIGLAMGVMAVA
                     T"
BASE COUNT         2235 a         3571 c         3255 g         2221 t
ORIGIN      
        1 gcatgacttg accttgcctt caattctagc gatgaaatcc tcgaatgttt caggtatgac
       61 ccgatgatga accgatttga aggccccggc ggcagagaag cacgcatccg ctatctcgat
      121 ggcgatttcc aggtaaccag ccccggcgct ttcgtgcgct gcgcggtcac cggcgaatcc
      181 atttcgctgg acgaactcaa atattggagc gtcgaaaggc aggaagccta tgccacggcg
      241 gcgatatcgc tcgaccgtga gttggccgcg catccggaat ggcgcacgcg cggctcgtcc
      301 gcagcctaac cctgcaaccg gcgacgacgc caggcttcca aggtttgctc gacgatccga
      361 tccggtatct ggtcggcttc gaacacggcg tcgatttcca gaacacttag tcggtcgaga
      421 aaacctgccg gcgcatgatc gtggcgcagc cgcatcgcgt ctttcgcggt ggtcaccagt
      481 tccagacccg ccacctttgc cgtttcggcc aactcggcaa gttcgtcccc ggcgtagaaa
      541 tgatggtctg gaaaaggccg cgccgcttca acgatgccgc cggcctcaac gagggtggcg
      601 aagaacttgt ccgggtggcc tatccccgca aaggcaagca ggtgcttgcc cgcaaacatc
      661 ttcttgtcac gcggctctgc gcgcgcttcg aaaatcggct tgccgccgcg cgcggccttg
      721 cgcaccagcg catcggcggc ctcgccgtcg cccatcttca ggacggcatc ggtgaatggc
      781 agttgatccg atattcttgc ccgaagcggc ccgcctggaa tgacatggcc gttgcccagt
      841 ccatggcggg catcgataac caccaatgca tagtcgatat ggatgcgcgc gctttgaaag
      901 ccgtcatcca tgatcaggaa atcgcagccg tgcttttcca gaaggatgcg cgcgccggcg
      961 gcgcggtccg gcgtcaccgc caccggcgca tgcgccgcca gcagcaacgg ctcgtcgccg
     1021 acatgccttg caccgtcgtg ctgcgcatcg acgacatgcg gctcgccaaa gctgccgcca
     1081 tgaccgcgtg acaatatacc gggtttcagc cccatctgct ttgcacgttg cgcaagcgcg
     1141 atggcaacgg gcgtttttcc tgtgcctccg accgtgaaat tcccgatgca aagcaccggc
     1201 gcgtcgatct tttcacgctt tgccgtcttc atgcggcgac cggcaaccgc gccataggcc
     1261 agcgaaagcg gcgacaggag ataggcgcgc caatccggct tttgccacca gaagggaggc
     1321 gcttcggaag ccacagccct accttgcgct tgcgtttttc aaccgcgcct tcaggaccag
     1381 cggctggatg tagggttcaa gcgccttgag cgtctttgcc agcgccccgc gcatttcctc
     1441 gacggttgca gcgccggccg ccatcatgtc gcgccgcgcg gcctcattgg taagcaggaa
     1501 attgacggcg ccaaccagca tcgcgcggtc gcgcaccagc tttgcgcccc cacggtcaag
     1561 caggcgctga taggattcgc ggaaattctg cacattgcgg ccagcaagca cggcggtttc
     1621 gagcatggcc ggttcgagcg gattctgccc gccttccgag gtcagcgaac gcccgacaaa
     1681 agcgatctcc gtcagccgga gataaagccc catttccccg atggtgtcgc caagcaggat
     1741 atcgacgtcg gggccgactt tttcgccggc gctccgccgt gcaaccttca agcccatctc
     1801 ggcaaactgc ccgacaagcg catccgctcg gtcggggtgg cgcggcacga taatcgtcag
     1861 caatcccgga tgccgggcgg aaagcgtcgt gtgaacttcg gcggcgatca cttcctcgcc
     1921 ctcatgcgtc gagacggccg cccaggtcgg cctcccatcg atctggcgct tcaagccatg
     1981 aagcgtcttt tcatcgaccg gcggcggctt ggtatcgacc ttgagattgc ccgatacgga
     2041 aaccggacgc gcgcccagct ccttgaagcg ttcgccatcc gcttccgact gggcaacgac
     2101 atgggccagg ttctcgaaaa gcgcctcagc gatgttggaa cgctttttcc acgatgcgaa
     2161 ggagcggtcg gaaagcctgc cattgactag cacctgcggc acatggcgtg cgccgagttc
     2221 cagaatggtc atcggccaga tttccgattc ggcaatgatt gcgaggtctg gacgccaatg
     2281 gttgagaaag cggctgaccg ccggcttgag gtcgagcggc acatattgat ggatgatccg
     2341 gtcgcccagc cgctcgtcgg ccaccttcgc cgaggtaatc gttcccgtcg tcagcacgat
     2401 gttgacgccg tagtcgagaa tgctctcgac cagcggcacc accgcgatgg tttcgcccac
     2461 gctcgcggca tgaacccaga tgagcggccc ttgagggcgc tcgcgctttg tcacgccata
     2521 acgctcgcgg ctgcggctgc ggtcttcctt gcccttggcc acgcgccagg cgacataggg
     2581 accgaccagc ggataagccg ccgcgcccgc gcagcggtac atcatcagca cgctacgtgc
     2641 ccagcgctcg ctcatcgcgg tccgtccacc atgcgataag cctgagcggt cgcggcgttc
     2701 agcgcctccg tcacttcctg ccgcttgcgc tccatctcgg cgtcgtcggc atctgccgaa
     2761 acgtagatcg gctggccgac aacggtggaa gatcgtccga acggcaggtt gatggtggtc
     2821 ttgtcccagc ttttctcaag aatcttgcgc cggctggtgg tgaaagccac aggcaggatc
     2881 gggcggcccg acagtcttgc cagaaggacg atgcccaggc cggaatcgcg cggcgtgccg
     2941 tggggaatat ctgcgatcat gcatacgttg gtgcctgccc gcagggtctt tttcaaggcg
     3001 atgagcgctt ttgcgccgcc cttgtcggat ttatgctcgc cttcccggcc gccggagcca
     3061 cgcacgatat cgatgccgaa cttcgccagc acgatggcgt tgagttcggc atcggcgctg
     3121 cgcgagacca tggcagcgag gccgctgccc tttggataaa agaccggagc cagcagatgc
     3181 tggccgtgcc agagtgcaac gatgaccggt tcgagatcgt gatactggcc gcctaatatc
     3241 ctgaccgaac cattggccag cggattggtg agccgcacga acttcaaacc caacgcaatc
     3301 aggctggcga cggcgccctt gaccgcgcgc gacttcgcca gcggctggcg aaccttcttc
     3361 cacagcgcct tcgttttgcc gcttcgcttt ttttccggca tggaattgac agcccgcgat
     3421 gtcgctacgc gttgatccat gtcaagcgtc ggccttgccg tccggatcga gcagccggtg
     3481 caggtggacg acgaaatatc gcatgtgggc gttgtccacg cttgcctgcg ccttggcttt
     3541 ccacaccgtc ttggccgttt catagtccgg gtagacgcca acgatgtcca gcgcgtcgag
     3601 gtcgcggaat tccgttccgc ccaccttttt cagttcgcca ccgaacacca gatgcagcaa
     3661 ttgcttcttt tcgctgtctc cagacatgtc gatccttcac atttcgtgat ttttcgtgtt
     3721 cacgacgtaa accaaaccgg ccctgttgaa aaccgcttta aaaatccgcc tgcgcgatca
     3781 cctcggtcat cgcggcaaat agcgcaccgc tgccggcagc cagcgcccca tggcgaatca
     3841 tttcgccggc ataggttggt gaattgccgt cttgcccgac cagttggccc ccggcttccc
     3901 gcaagatcag gtcggccgcc gcaatgtccc agtcatgggc gttgggcttt acaaacgtcg
     3961 catccagcac tccgctggcc accatcgcaa ggcggtaggc cagggaagga atatacggcg
     4021 cgcgcgccaa atgccgctgc cagtcaggcg ccagcagatc gatcagcgcc ttcggtccgg
     4081 cgatttccag tggcggggtc gcgtcgcgcc ttatggagat ggctttcccg ttgagaaacg
     4141 caccctcgcc cggcatcgcc caataggttt cctgtttggc cgggcattcc agcacgccgg
     4201 caatcgtctt tccgccttca accacggcaa cgcagacgca ccagctatcg agaccttgca
     4261 gaaatccgcg cgtgccatcg atcggatcga cgacgaaggt gcgccgcgcc gacaggcgga
     4321 ccggatcgtc ggcggtttcc tccgaaagcc agccataatc ggggcgcgcc gcaagcagcg
     4381 tcgtgcgcag atatttgtcg gcggcataat cggcctcgct taccggcgag gtgccgccct
     4441 tcatccagac ctcgggatcc ttgccgaaat aattcatggc gatcacgcct gcctcccgcg
     4501 ccgcatcgag cagcagcgcc agatcgctgg ctgcgctggg caaatcggtc gggtcagctg
     4561 ccggcaaggg tcatgccttc gatcaacagg gtgggcgccg ccgttccgaa attccggtcc
     4621 aggtcgcttg ccggcaccat attcaggaac atcgtcttga ggttcgaggc gatggtcacc
     4681 tcggataccg gataggcgag cgcgccgttc tcgatccaga aaccggaagc gccacggcta
     4741 tattcgccgg tgatcatgtt aacgccctgc ccgaacacct cggttacgta gaaacccgat
     4801 ttcatggagc cgataatatc ctccggcgtc ctgtcgcccg gctcgattgc gagattggtc
     4861 gatgacggcg acaccgatga cccgccgcgc gagccgcgac cattggtcgt cagccccagt
     4921 tcccgcgccg ccgaagtcga caggaaccaa tggttcaaga agcccttctc gaccatgatg
     4981 agcttttcgc cctcaatgcc ctcgccgtcg aacgggcgcg aagcctggcc gcgccggcgc
     5041 agcggctcgt cggtgaccgt gatggcggcg gccgccacct gtttgcccat catttcgcgc
     5101 agaaaactgg ttttgcgcgc aaccgacgcg ccgttgatcg cgccagccag atgtcccgca
     5161 atgccgcgcg atacgcgcgg atcgaaaacc acgtcgacgc ttccggttgc cgccttgcgc
     5221 gcgcccaggc gccgaacggc gcgctcaccg gcgcttcgcc caatggtctg cggcccgtcg
     5281 agatcggaga aatgctggcg agacgaaaaa tcgtagtcac gctccatgcc ggtgccctcg
     5341 cccgcaatca cgctggccga acgggaaaag cgggaagcga catactggcc gacaaaaccg
     5401 tttgaagtgg caagaaccag cccgccaagc ccagcgcttg cgctgctgcc gcctgaattg
     5461 gtcacacctt tgacggcgag cgctgcttcc tcggcggcca gcgcgtcttg tttcaaccgc
     5521 tcggccgata cttccgtgcc gtcgaacaga tcgagatcgc gcggcgactt taccagcagc
     5581 gccgtatcgg caagcccctg atagatatcc tcgggcgaaa ccttagccat ggcgaccgca
     5641 cgttcggcca atgccttggg gtcggacgat gcggttgccg aaacgctggc aacgcgcgcg
     5701 ccgacgaaga cacgcagcgc cacatcctcg ctttcggatg cctcggtcgc ctcgaccttg
     5761 ccgaggcgga ccgacacgcc ggtagagcgg ccgcgtaccg caaccgcatc ggccgcatcc
     5821 gcgccggcgc gcttggccgc ttcaaccagc gccgtcacgc ggtccgtcaa ttttgctgca
     5881 tccggtgcgt cggtcatcgc gtaatcctgc ctgttttggg gcctgcctgg ggcgcggccc
     5941 gccactgcac catctattgt tccggtccgg cttgtgcaat gggagaagcg gcaaacctcg
     6001 ccgccggctg gctttatctg ctatgggatg accagccgag atcgatacaa cagcacggaa
     6061 tatccgaaat gacacccttt caccttgcat ttcccgtcac cgacctggat gaaacgcgcg
     6121 ctttctacgg ggaagtgctg ggctgttcca tcggacgatt ttcagaaacc tgggtcgatt
     6181 tcgatttgtt cggacatcag atgtccgcgc atctgagacc gcaggccacc gcagccgccc
     6241 gcgatggcaa ggtcgatggg gttgtcgtgc ccatacctca tttcggcgcg gttctgctga
     6301 tggacgagtg ggagcgtctg gctgcgaggc tggtggcgcg cgatgacatc gactggctgg
     6361 aaaagccgat ggtccgcttc aaaggtgaac ccggcgaaca agcaacgctg ttcattcgcg
     6421 acccctcggg aaacgcgctg gagttcaagg gtttccgctc gcttgagcag atctttgcgc
     6481 attgaccgca aggcttgcgg tcgaaagaac tgaaaagccg tatggtttcg gctaggcgtg
     6541 atcgccttag atggcggttc ccagcaccca ctcgaccagc gccagccccc atacggcggc
     6601 cgccatcacc acggaaatca ggacggcaag cgaaccgcag tccttcgaca gctgaatatc
     6661 ggcgttgaat tcgcggctga cggcgtcaca gaccgcctcg atcccggtat tcaaaacctc
     6721 gaccacgatc agcaacagca cggcgccgat cagcagcgca taatgttgcc atgaggagga
     6781 aagaaaccat ccggccggaa atgccagcaa caatacgatc agttcctgct ggaaggcctg
     6841 ctcggtggcg gccagccggc caaaagcccg gcaggaattc acgaatgcgc tgacaagacg
     6901 gttcatgcca agtgtccttt cggcgtgacg gcaggaaaca cgagcgccat gccgcttcaa
     6961 cggaaagacg cgcttatcca ttgtctttca gtcttgcgca accttgaccg atggcgacga
     7021 tttctcgaac tggcgaagac cgtcgtcgat ggtgtaatag tcgcccttgt cgccggtaaa
     7081 tatatggctt tttccagcca gccccgaagg ccggtcgaag gagcctgcca tgatggagat
     7141 cgagtccaga tcatggtgct tccagaacat caccgagccg cacgtaccgc aaaaaccgcg
     7201 cttggccgcg tcggacgcgc cataccattt caatgcctcg ccaccctcga tgacgatatc
     7261 ggcatccttg gaattcgtcg ccgcgacgac atgtcccgtc tgcttcctgc actgcgagca
     7321 atggcaataa atgacccccc gcaaattacc gtgggtgcgg aaatggacgg cgccgcaaag
     7381 gcatgatccc gtgcgcatag tgctcatgac gaactccgtg atctgaggct gacgcaggat
     7441 tccagtttgg ccacggccat gatggaataa atgcgacatc ggatcgccct atcggcaaat
     7501 tcaaaaaaac cggtccaggg cttcgccaca atttcaggta gaagtcgggc tgattcaatt
     7561 gcggaattaa caaagcttta gcaacgctgc atttaaggtt cggttgtggg cggcgctttc
     7621 ttgggcgcgc ctcccggccg gcgacatcga cgttttttaa aatatggcgc cgggttcaaa
     7681 cgggcggggt ggatcagaca tgcagccggc actggctcag actgaacgag cgacggatat
     7741 tgccacgacc atcgttgcca ccatgcgcca actcggcgtg ttgggattgc cgcgcaatta
     7801 tgaaatcttc tacgaggcgc tgaccggttc gaacagcgag cttcgtttgg ccgtggtttc
     7861 gcttggcgac aagccgaccc aggaagaact tgaccgcatc ggccaccgct tcttcgcgca
     7921 aaatcatggc cacggcattg tcgagcaggc gcgcgatgta attgccaagg aacttgaaga
     7981 catcgcgctg ctcctgcgca gcgagcgcac ccatatcgag aaatatggca agatcctcaa
     8041 cgaaacgtcg agcggcctga gcaaccgcaa tgtaatttcc aacgatctct tgcagaagat
     8101 cgtcagcgcc atgtccgtcg ccacgacatc gacaatcgat catggcaagc atgtcgccac
     8161 cacgcttacc gaaaagaccg ccgagcttga aagcgtgaag accaagctcg aagaatataa
     8221 gcgcctggcc gacaccgacc cgctgacaca aatctggaac cgtcgcgcct tcgacaagga
     8281 aatcgccaaa atatacaaca gcaacaaggg catcttgttc aacgccctca tcttggcgga
     8341 tatcgaccgt ttcaagaata tcaatgaccg gttcggccac ccggtgggcg acaagatcat
     8401 ccagctcatt gccgaaatct tccagaccgg cgttcgcggc gacattttcg tggcccgcac
     8461 cggcggcgag gaattcgcgc tgattgtcga gggcgcgagc gaggacacca cttatgaaat
     8521 cgccgaccgc attcgcgcct tgatcgaaca gaccccgttc gccagcagct cgaccggcgc
     8581 cagctgcggc tcggtgacgg tatcgatggg catgtgcatg gcgtccgaag ccgacggccc
     8641 cgaggattta tacaccaagg cggaccgcgc gctctatcgc tcgaaagtca gtggacgcaa
     8701 tcaggtcacc cgacattcat ccatggcagg ccgcaccagc aagaactggc ttctgtacaa
     8761 aaaggactaa ggtccaccca taagacgaaa taaaaaggcg cctcgcggcg cctcttttat
     8821 ttctggcctt gccttgttca ggctgtcatc gcccgcttgt gcaaaacggg ctgggtcttc
     8881 ttcaacaggt ccgataccag gaatgcaagc tcgatcgcct ggtcggcatt caggcgcgga
     8941 tcgcaatgcg tgtggtagcg gtcctgcaat tcctccgccg tgatggcgcg cgcgccaccg
     9001 gtacattccg taacgttctt gccggtcatc tcgatatgga tgccgcccgg atgcgtgcct
     9061 tccgcgcgat gcacttcgaa aaacgtctgc acttccttca ggatacgatc gaacggccgc
     9121 gtcttgtagc ccgccgcagt gatcgtattg ccatgcatcg ggtcacatga ccaaacgacg
     9181 ctgcggccgg cctttttcac ggcctggacc agcttcggca ggtggtcgcc cgccttgtcg
     9241 gcgccaaagc gcgcgatcag cgtcagcctt cccggctcgt tctccggatt gagcaggtcg
     9301 atcaactcca tcaggccgtc ggccgtcatc gacggtccgc acttcagccc gagcgggttc
     9361 ttgatgccgc ggcaatattc gacatgcgcg tggtccggct ggcgcgtgcg gtcgccgatc
     9421 cagatcatat ggcccgaagt ggcgtaccag tcgccggatg tcgaatcgac acgcgtcagc
     9481 gcttcctcat agccgaggag tagcgcctca tggctcgtat agaagtccgt ctcgcgtagc
     9541 gcgtaattgg tctccgacgt gatgccgacg gccttcatga agtccatcgt ttcggtgatg
     9601 cgattggcaa gcgactcata tttctcgccc tgcgggctgt cggaaacgaa accgagcatc
     9661 cagctgtgca cgttttcaag gctggcgtaa ccgccctgcg caaaggcgcg aagcaggttc
     9721 agcgtcgccg ccgactgccg gtacgccatc acctgccggc tcggatcggg aatgcgggac
     9781 ttcgcatcga aatcgatgcc gttgatgatg tcgccgcgat agctgggcag cgtctcgtcg
     9841 cccttggttt cattgtccga agagcgaggc ttggcgaact gcccggcaac gcgcccgatc
     9901 ttcactacgg gctgcgaacc ggcaaaggtc agcaccaccg acatctgtag gaagacgcgg
     9961 aagaagtcgc gaatattatc cgcgccatgc tcggcgaagc tttcggcgca gtcgccgccc
    10021 tgaagaagaa atgcgtcacc ggcagcgacg gtcgccagct gttttttcag cttgcgcgcc
    10081 tcgcctgcaa aaaccagggg cggaaaggtg gccagttggg cttccgtggc cttcagcgca
    10141 ttcgcatccg gataggccgg aacctgctga attggcttgg ctctccagga attcggggac
    10201 catttcgtca tcgtagcacc aacgctcttt cccgcggtcg caatatcccg cgattttccc
    10261 tttgcctata tggggatcgc cggctccata cacgatgccg gatttgtatt ctagaccgga
    10321 tttaagtggc tggcgatctg ccgggaccaa cccgaatcaa atttcaaacg ttaccgtcat
    10381 gtcgtttgcg gcgaccaaaa cggtcatttt cttcactaaa agtggcagcg gatcgcatca
    10441 aagccttatg cacatcctcc gatagctgag aatctttggc ttttcctttc ggccgcgctg
    10501 acaggcgctt cgccatatcc ggctcttttg agcgcgttat ctaactgtaa caaaacgttt
    10561 ttatatcatg cgcaattcgc gtcgcgtttc attgtgttgc cgctcggcaa cgtttttcgg
    10621 tccagacaac gccttgccga caaaattaat ggaaaatgat caattggtgt taccatctgt
    10681 aacaaagaac gagcgtgttt ggatgggatt gtgattcgcg acgaccgacc gcgacattca
    10741 atactgagac gactgcaccg tggagccgaa aggccgccgc ccctgacgcc ggtgtcttca
    10801 atgcgcggct tctgggggct gatccgggcc tactggtttt cggatcactg gaaggaagcc
    10861 tggagcctga cgattatcat cgcagcgctc acggcaatct ccagcaagac gggcgtttgg
    10921 ttcgccgaaa tgtcgggcga actggtcaat tcgatagctt tctaccacga cgccgcaaac
    10981 accgcgccgc tgcggtccct cctgaccaac gccggcatgc tcgttgtcct ggtcatcatc
    11041 aaggacgcag gcattaccgg caccaaaagc ttcgtatcct cgaccctgca ccgaaaatgg
    11101 cgcaaatggt tgaacggacg tttcaacgaa gcccttcttg accccaatca tacgcatttc
    11161 cacacccaga acgggtccga cggctccgtg ccggacaata tcgatcagcg cgtgcaggaa
    11221 tcgatcaagg gcgtcaccgg cggcgccatc ggcctggcca tgggcgtcat ggccgttgcc
    11281 ac
//
LOCUS       DASYCS010000454         1616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3326299,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000454 DASYCS010000000
VERSION     DASYCS010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3326299"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..922)
                     /locus_tag="VGM46_13990"
     CDS             complement(32..922)
                     /locus_tag="VGM46_13990"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional helix-turn-helix transcriptional
                     regulator/GNAT family N-acetyltransferase"
                     /protein_id="HEY4193752.1"
                     /translation="MDRNQIEQVRLFNRLVTQRVGALQDSYLSRGRPLGEARLLFEIG
                     VEGRDVRQLRALLNLDSAYVSRLLRSLEAQGLVAVAKQAGDGRVRRAVLTAKGRAEFD
                     SYDQLSDELAASILSPLDSSQRARLTAAMSEVRALLSIPQIEIATVLPDEPDARWCLD
                     QYFRELAERFETGFDPDAGKSLSDAEMMPPNGFFLIARSSDEPIGCAALVRLDAETAE
                     VKRMWTAPSARGQGLARRMLRELDTMARGEGYRKLVLDTNRILREAQALYRSEGFIET
                     ARYNDNPYADFWFEKNLLAR"
     gene            complement(978..>1616)
                     /locus_tag="VGM46_13995"
     CDS             complement(978..>1616)
                     /locus_tag="VGM46_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008597511.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEY4193753.1"
                     /translation="EIFEKEDPAPYLEKVNGILVPGGFGERGSEGKILAAKFARERKV
                     PYFGICFGMQMACIEAARSLAGIEHASSTEFGATDEPVVGLMTEWLKGNMLEKRRETG
                     DLGGTMRLGAYEARLSPDSKIAEIYGSAEISERHRHRYEVNIDYKQRLEECGLVFAGM
                     SPDGVLPETVEYADHPWFIGVQYHPELKSRPLEPHPLFASFINAAVEQSRLV"
BASE COUNT          309 a          527 c          468 g          312 t
ORIGIN      
        1 agggccgcgc caacacgcgg ccctttttct gtcagcgcgc aagcaggttc ttttcgaacc
       61 agaaatcggc atagggattg tcgttgtagc gcgccgtctc gataaagccc tcgctgcggt
      121 agagcgcctg tgcctcgcgc aaaatccggt tggtatcgag gacaagcttc ctgtagccct
      181 cgccgcgcgc catcgtgtcc agttcacgca gcatccgccg cgccaggccc tgcccgcgcg
      241 cggacggggc ggtccacatg cgctttactt ccgccgtctc cgcatcgagc ctgaccagcg
      301 ccgcgcagcc aattggttca tcgctgctgc gcgcaatcag gaagaagccg ttgggcggca
      361 tcatttcggc gtcggacaat gacttgccgg catccggatc gaagcccgtc tcgaagcgct
      421 ccgccaattc cctgaaatac tgatctagac accagcgtgc atccggttcg tcaggcagca
      481 cggtggcaat ttcgatttgc gggatggaaa gcaacgcgcg gacttccgac atggccgccg
      541 tcaagcgggc acgctggcta gaatccaagg gcgaaagaat ggaagccgcc aactcatccg
      601 acagttggtc atagctgtcg aattcggctc ggcccttggc ggtcagcacc gcgcgccgca
      661 cacggccgtc gccggcctgc tttgcgaccg cgacaagacc ttgcgcctcc agcgaacgca
      721 gcaggcggct gacataggcg gaatcgaggt tcagcaacgc gcgaagctga cgcacatctc
      781 gtccttcaac gccaatctcg aaaagaagcc gcgcctcgcc aagcggcctg ccacggctga
      841 gatagctgtc ctgcaatgcg ccgacgcgct gcgtgacaag gcggttgaac aggcgaacct
      901 gctctatctg atttctgtcc attacctgac tttagtcagg taaatctcac cgcgcaagat
      961 ttctctcgca ccgatcgtca gaccagacgg ctttgttcta cggcggcgtt gatgaagctg
     1021 gcaaacagcg gatgcggttc aagcgggcgg ctcttcagtt cggggtgata ctgcacgccg
     1081 atgaaccatg ggtgatccgc atattcgacc gtttccggca gcaccccgtc cggcgacatg
     1141 ccggcaaaca ccagcccgca ttcctccagc cgctgcttgt agtcgatatt gacctcgtag
     1201 cggtggcggt ggcgctcgga aatttcggcg ctgccgtaaa tttcggcgat cttcgaatcc
     1261 ggcgacagcc gcgcctcata agcgccgagc cgcatcgtgc cgcctagatc gcccgtttcg
     1321 cggcgctttt ccagcatgtt gcctttcagc cattcggtca tcaggccgac aaccggctcg
     1381 tcggtggcgc cgaattcggt ggaggatgcg tgctcgatgc cggccagcga gcgggccgcc
     1441 tcgatgcagg ccatctgcat gccgaaacag atgccgaaat acggcacctt gcgctcgcgc
     1501 gcgaatttgg cggccaaaat cttgccctcg gaaccgcgtt cgccaaaacc gcccggcacc
     1561 agaatgccgt tcaccttttc cagatagggc gcgggatctt ccttctcgaa aatctc
//
LOCUS       DASYCS010000455        28813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2746373,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000455 DASYCS010000000
VERSION     DASYCS010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 28813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28813
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2746373"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VGM46_14000"
     CDS             <1..531
                     /locus_tag="VGM46_14000"
                     /inference="COORDINATES: protein motif:HMM:NF026098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase dimerization
                     domain-containing protein"
                     /protein_id="HEY4193754.1"
                     /translation="ISMQTLLAKTGAERVVRAMPNAAAEIGLSYTPWFCSAAVSQADR
                     AFVQSLFESCGKADEVAAEAEIDYLTALSGSGPAFPALLADALLGHALSQGLPAAIAR
                     RAVEGVVCDAGRLIAASDATPGEMVRTFMEYRGTTDAGLRAMKNAGFAEAIHAGLSAA
                     AAKAVAMENHGNRSEE"
     gene            597..1598
                     /locus_tag="VGM46_14005"
     CDS             597..1598
                     /locus_tag="VGM46_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011649278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY4193755.1"
                     /translation="MKKLLASTCLTMGLLTMASFASAAECGNVTIASMSWQSAEVMSN
                     VDKVILEKGYGCNAEIIVGDTVPTITSMVEKGQPDVAPEAWIDLLPEVVDRGMKEGKI
                     VTAATSLPDGGVQGWWIPKYLADAHPDIKTIADALKHPDLFPAPEDASKGAILNGPAG
                     WGGTIVTSQLFKAFDGEKAGFTLVDTGSAAGLDGAIAKSYERKQGFAAFYWAPTALLG
                     KYEMVKLDNGYPVDKAEWARCNTVADCPDPKVNGWPKDRVFTLVTKPFADRAGPQVMD
                     YLTKRSWSNDTVNKLMAWMTDNQSTGADGANHFLKENPDLWAKWVSPEAAEKIKAGL"
     gene            complement(1795..2556)
                     /locus_tag="VGM46_14010"
     CDS             complement(1795..2556)
                     /locus_tag="VGM46_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEY4193756.1"
                     /translation="MIELTDIEKRFGENHVLKGVGVTIAEGSVTALVGPSGGGKSTLL
                     RCINLLEIPTSGTVRIGDEALTFKPGVKVATKDIQRVRRQTGMVFQSFQLFPHRTAIE
                     NVMEGLTTVLKWPAERAKKRAMELLEKVGMEHKADAWPATLSGGQQQRVAIARALAPS
                     PKVLLCDEPTSALDPELAQEVVDVLGQLAGEGTTMVMATHDLRLASKIAREVVFLDAG
                     VVVETGPAGELFGNPKRERTKRFIATLRQEANESP"
     gene            complement(2553..3233)
                     /locus_tag="VGM46_14015"
     CDS             complement(2553..3233)
                     /locus_tag="VGM46_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535752.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY4193757.1"
                     /translation="MPHWLQLMLDSLGPLLWAAAVFTTPLTLLSFAFGLGVGLVVALI
                     RLFGPKPLVAVVRFYVWIFRGTPLLVQLFLIFYGLPSAGILLDAFTAALIGFTLNVGA
                     YTSEIIRAAIGSVARGQWEAAYSIGMSWSQAMRRTILPQAGRVAVPPLSNTFISLVKD
                     TSLAAAITVPEMFQAAQRIVAVTYEPLILYVEAAILYLAMSSVLSALQTRLEVRLDRY
                     GGFLEARS"
     gene            complement(3363..4136)
                     /locus_tag="VGM46_14020"
     CDS             complement(3363..4136)
                     /locus_tag="VGM46_14020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007827550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY4193758.1"
                     /translation="MKWLNSLIVAGAVQALAITASFAGANLDQIKADGAIKIGTEGTY
                     APFTYHDENNKLVGFDVEIGEAVAAKLGVKPDFLEGKWDGLIAGLDGKRYDAVINQVG
                     ITEERKAKYAFSDPYIASKAVLIVKGDNADIKSFADLKGKKSAQSLTSNFGKLAEKNG
                     AELVGTDGFDQSIQLLLTGRADATINDSLSYLDFKKHKPDADVKIAAQEENADYSGII
                     VRQGDPELVAAINKALADIKADGTYQKIADKYFGQDVSK"
     gene            4163..4357
                     /locus_tag="VGM46_14025"
     CDS             4163..4357
                     /locus_tag="VGM46_14025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193759.1"
                     /translation="MTPTLVTGGNLTERLRQIKEIYSNFSPLQESNSDFPPFNAYTHH
                     SGLQIAGNHFSRKPVIATRA"
     gene            4427..5284
                     /locus_tag="VGM46_14030"
     CDS             4427..5284
                     /locus_tag="VGM46_14030"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-32"
                     /protein_id="HEY4193760.1"
                     /translation="MMQDSAGRSFIRSAMQAPYLERDEEHLLAQRWRDDADQNALHRM
                     TVAHMRLVISMAMKFRHFGLPAGDLIQEGHVGLLEAAARFEPNREVRFSTYATWWIRA
                     SMQDYILRNWSIVRGGTSSAQKALFFNLRRLRARLAAGSGPLGNEMLYRQISTALGVS
                     EADVATMDSRLSAPDSSLNAPLTDDAGAVERMDFLVADDPLPDEVVGDTIDVERRAVW
                     LKSALTALDARELRIIEERRLTDEGATLEALGEKLGISKERVRQIEVRAMQKLKTALV
                     RQNPEFLAA"
     gene            complement(5399..6877)
                     /locus_tag="VGM46_14035"
     CDS             complement(5399..6877)
                     /locus_tag="VGM46_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEY4193761.1"
                     /translation="MNPLARYLALAGFVLALTGALAGCQAVTPDIQESGFQPSNRPVT
                     VDNVAANSKLAELAKAQHPRILATYGGEYSDPKLERMVAKVVGSLTTVSANPSQTYRI
                     TILNSPNVNAFALPGGYLYITRGLLALANDSAELAAVIAHEMGHVTANHGLQRQQKEA
                     EEVLATKVVSDVLGDSPTAKAALIRGKLRMAQFSRNQELEADAIGIKSVGEAGYDPYA
                     AGRFLQSMSAYSDFRSISGATDASLDFLATHPNTPQRIELAQRHAREFGQPGVGTVDR
                     NSFLAGIDGLLYGDTPDEGYVRGNTFMHPRLGISFSVPDGFIIDNSAAAVTATGPGDM
                     AIRFDGVAIDQNRSLPDYIRSGWVAGLDNASVHEETIAGNEAATAKASADGWRFDIAV
                     IRANGQVYRLLTAAPIASTSLDTVARSVSGSFRVLDAAQKAALKPLHIRVVTVQPGQT
                     VGSLSAMMIGVDRKLDLFLVLNAMSPGASVSAGDKVKIVTDK"
     gene            complement(7085..7846)
                     /gene="thiQ"
                     /locus_tag="VGM46_14040"
     CDS             complement(7085..7846)
                     /gene="thiQ"
                     /locus_tag="VGM46_14040"
                     /EC_number="7.6.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006327789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA];
                     GO_process: GO:0030974 - thiamine pyrophosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein"
                     /protein_id="HEY4193762.1"
                     /translation="MNTGMEKEAWGVRLDEVSFSYGETALSFDVAFAPATITAIMGPS
                     GSGKSTLLNLIAGFETPRAGRVLIGQKEVTADQPSMRPVSMVFQENNLFAHLSVEQNV
                     GLGRSPGLKLGAADRAAIIDALARTGLSGKERRLPRELSGGERQRVALARVLVRERPV
                     LLLDEPFASLGPALREDMLDLVAGLQAERAMTVLFVTHQPEDARRIARDMVFLENGRV
                     AAAGSAADFFEGSGPEVFRRYMGSQLAGGRSRDIA"
     gene            complement(7843..9519)
                     /gene="thiP"
                     /locus_tag="VGM46_14045"
     CDS             complement(7843..9519)
                     /gene="thiP"
                     /locus_tag="VGM46_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA];
                     GO_process: GO:0030974 - thiamine pyrophosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease"
                     /protein_id="HEY4193763.1"
                     /translation="MGLIDPSPRLADAASRHLLAGSPRKADPRIAAGIIALAFVVLLV
                     GGAFAGLVVQGGQDISGAVSAFDGYLFRVARFTLRQAGLSTVLSVAPALLVARALSRH
                     PRFFGRSLILQLFAVPLALPAIVAALGVLSLYGRAGYFAGIFSAIGGEGWPGIYGLSG
                     ILVAHVFFNLPLGVRLFLEALQTVPADQWRLAGQLGMGPRATFRLIEWPALRQALPGV
                     AGLVFMLCVTSFTIVLTLGGGPRATTLEVAIYQALRFDFDPSRAVALTALQVALTFAV
                     VLALARLGANTTGDANLPVAPRRFLTPGRPETALDITLIALALLFVAGPMAATVMAGL
                     DADLTRLAAEGAVRRATLTSIVLAVLSALLSAMLSFSLVAARRALTLGRGGGIKGALE
                     YATDTGAGFVLVVPPIIIGAGWFLLLRRFADVFAIAPVMVVTVNAVMAMPFAVRAIRP
                     AYDAASERHERLCALLGIAGWNRLRLVDWPALRRPFATAFAFAMALSLGDLGVIALFG
                     GDSVQTLPYLLLARMGSYRTADAAGLALLLGLVCLALMLAADRLGKDRPS"
     gene            complement(9602..10597)
                     /gene="thiB"
                     /locus_tag="VGM46_14050"
     CDS             complement(9602..10597)
                     /gene="thiB"
                     /locus_tag="VGM46_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA];
                     GO_process: GO:0030974 - thiamine pyrophosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HEY4193764.1"
                     /translation="MRTFFYSLIPAIALLAVPAAAKEKLTVYTYESFTAEWGPGPQVK
                     KAFEAECGCEVDFVSVADGVALLNRVKLEGASTKADVVLGLDTNLTAEAKATGLFAPH
                     GSVAETSVPGGWTDDIFVPYDYGYFAVVYDTQAMKNPPKSLKELVEGDPSQKIAIQDP
                     RTSTPGLGLLLWVKSVYGDKAPEAWARLKDRVLTVTPGWSEAYGLFTKGEAPMVFSYT
                     TSPAYHMIAEKTDRYQAASFSEGHYLQIEVAGTTTVGAKNPLSEKFIAFMTGPQFQDA
                     IPETNWMFPAGKTDKPLDPAFDKLVKPAKTLLFSADEVAKNRKAWVDEWLAVMSK"
     regulatory      complement(10640..10754)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            10856..11500
                     /locus_tag="VGM46_14055"
     CDS             10856..11500
                     /locus_tag="VGM46_14055"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEY4193765.1"
                     /translation="MSQFTILLGGDLVHTPLLARQVAGTRTIAADAGIGHAAMLGLMP
                     ELWVGDFDSVPPNLPAHLASVPRQTFPREKDKTDGELAIATALQRGATSLILAGAFGG
                     ARTDHAFLHLALAVHLAEAGTEVLLTSGAQEGIPVLRGERSFDYADGTLFSILGFSDL
                     SALTVEGAKWPLDNVEVAFGSSLTISNEVRGRLRVELGDGRAMLLAHPFPVAGS"
     gene            11504..13318
                     /locus_tag="VGM46_14060"
     CDS             11504..13318
                     /locus_tag="VGM46_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002969184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEY4193766.1"
                     /translation="MAPPLLNLDAIKLTFGGTPLLDGAELTASAGDKIALVGRNGSGK
                     STLLKIAAGLVEPQDGEVFRQPSATIRYLPQAPDMEGFATVRTYVEAGLGPADDPHRA
                     TYLMEHLGLSGEERPADLSGGEARRAALARVMAPEPDILLLDEPTNHLDLGVIEWLEE
                     ELVRTSSALIVISHDRRFLERVSRATVWLDRGQTRRLDKGFGHFEAWRDQVLEEEERE
                     QHKLGRQIVREEHWLRYGVTARRKRNMRRLGELQTMRQQFRGHRGAEGSATMVASDAT
                     ESGKLVIEARNIGKSFGDLTVVDGFSTRIQRGDRVGLVGPNGAGKTTLLKMLTGELKP
                     DAGSVRLGTNLEIATLDQKREAVDPAETLAQYLTDGRGENLVINGEQRHVVSYMKDFL
                     FKAEQARTPVRELSGGERARLILARVLARPANLLVLDEPTNDLDMETLELLQELVAGF
                     AGTVILVSHDRDFLDRTVTSVIAPDGNGSWIEYAGGYSDMLAQRGVTRLEGRKARVKA
                     DDAATANRAEPSAPKAPSKKLSFKQKFALETLPKKIEAVTQSIARLENNIADPAYYER
                     DPASFQKTIAALDKERTTLAALEEEWLELEMLREELEG"
     gene            13507..13776
                     /locus_tag="VGM46_14065"
     CDS             13507..13776
                     /locus_tag="VGM46_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018495728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HEY4193767.1"
                     /translation="MRSFIGDLAMLGKRQSPQRKPTNLSLDATLVAEAKDLNINISRI
                     AEDGIARAVSEEKARLWRIENRDSIQSLNAYVEEHGIPFENDRQF"
     gene            13776..14075
                     /locus_tag="VGM46_14070"
     CDS             13776..14075
                     /locus_tag="VGM46_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="HEY4193768.1"
                     /translation="MARYDVFSNPTGPGYLLEVQADLLEDLNTRVVVPLVPHDVKLKA
                     VRRLNPVFMIEGRQYVLFTHLIATIPAVRLTGPRTNLIRHHDEIVTALDMLFQGF"
     gene            complement(14100..15683)
                     /locus_tag="VGM46_14075"
     CDS             complement(14100..15683)
                     /locus_tag="VGM46_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193769.1"
                     /translation="MADFVAVLKKTIDGMGDTTPDVRARVYDKARSTIAAKLAAIVPP
                     PPEAVAERQKRTLEEAIATVEKGYAKPAPAPAPASDPLAELENIFSSIDRNKNQPSHL
                     RPAPRIEPAWQAPVPETRKAPEPVPTPAPAPSFAPAAEKRTDREAARPAEARPAVRDE
                     AFSPYPDTDPEADVFEPAGSSERSRGRGGLVAAVVALLVVAGGAYGIWLNAGAFKSMF
                     GAGKAGGEVVKEEPLAAAKPAAPTAPTTQPGQATPANTATDTGEAPKFTQRLTSEGKE
                     TDPGPAGGENTIGEGTSVVGLTTPPPAQGAAPPAGAAAAVGGVTAPAAGTTAAPAAAP
                     AAAPAQSALAVGQKAIFYEERTSAAQGSAESGNIVWSLVQESPGGDAPPEPAIRAEVT
                     IPGKDVQLRMTIHRNTDETLPASHIIELIFLTPDGFEGGGIDNILRVAMKPAEQDAGS
                     PLVGIPAKIADGFFLVALNDTKADEQTNLTLLRNQGWIDIPVIYKSGRRALLTMEKGI
                     PGEKVFDDAMKAWQAKTAG"
     gene            complement(15757..17055)
                     /locus_tag="VGM46_14080"
     CDS             complement(15757..17055)
                     /locus_tag="VGM46_14080"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018481713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEY4193770.1"
                     /translation="MANVVVVGSQWGDEGKGKIVDWLSERADVVVRFQGGHNAGHTLV
                     VDGTVYKLSLLPSGVVRKGKLSIIGNGVVFDPHAFVAEMAKLKAQGVEVTPDRLKIAE
                     NTALILSLHRELDGFREDAASSSGTKIGTTRRGIGPAYEDKVGRRAVKVMDLADLETL
                     PLKVDRLLAHHNALRRGLGHEEVSREAILGELTSVADQILPYVDRVWKVLDDRRRAGE
                     RILFEGAQGTMLDIDHGTYPFVTSSNTVAGQAAAGSGVGPGAINYVLGITKAYTTRVG
                     EGPFPTEQTNEIGEFLGTKGHEFGVVTGRKRRCGWFDAVLVRQAVAVNGIKGIALTKL
                     DVLDGLDEIKVCTAYRLDGETIDYLPASQGAQARVEAVYETLEGWKGTTAGARSWNDL
                     PAQAVKYVRYIEELIGAPVALLSTSPERDDTILVTDPFQD"
     gene            17257..18156
                     /locus_tag="VGM46_14085"
     CDS             17257..18156
                     /locus_tag="VGM46_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008128070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY4193771.1"
                     /translation="MHKKAYLLLLATTLFWAGNTVAGKLAVGHISPMLLNAARWSFML
                     AALLVFGRRHLARDWATMRRHWPFLLFAGFIGFTLFSVAMYSALLYTSATNVSIEQGG
                     IPLFVFVASYLLFGVRTTAGQAAGFALSFAGVVVTATHGEIHSLLHLGMNYGDALMLL
                     AIIAFGFYTALVRRKPDIHWISLMAALCVGAVVSAIPFVALEAASGTLVPPDGQGWLV
                     VFYAVVFPSLAGQAFYVRAVEMIGANRAGIFVNFLPVWGALLAVAILGEEFHAYHAIA
                     LFIVMAGVFLAEFSGHRAQSARI"
     gene            complement(18266..18850)
                     /locus_tag="VGM46_14090"
     CDS             complement(18266..18850)
                     /locus_tag="VGM46_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006327799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEY4193772.1"
                     /translation="MATAIPQKKVSTSARNGFKTGEFIVYPAHGVGQIVSIDEQEVAG
                     HKLELFVIDFQKDKMRLKVPVAKATSIGMRKLSEEDYVERALKVVQGRARVKRTMWSR
                     RAQEYDAKINSGDLISISEVVRDLYRADNQPEQSYSERQLYEAALDRMGREIAAVNRM
                     SETEAVRLIEVNLNKGPKRGAKADNEEAEQEEAA"
     gene            complement(19214..19552)
                     /gene="fdxA"
                     /locus_tag="VGM46_14095"
     CDS             complement(19214..19552)
                     /gene="fdxA"
                     /locus_tag="VGM46_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HEY4193773.1"
                     /translation="MTYVVTDNCIKCKYMDCVEVCPVDCFYEGENMLVIHPDECIDCG
                     VCEPECPADAIKPDTESGLDKWLQLNSEYADKWPNISAKKDAPADAKSFDGETGKLEK
                     YFSAEPGEGN"
     gene            complement(19716..20102)
                     /locus_tag="VGM46_14100"
     CDS             complement(19716..20102)
                     /locus_tag="VGM46_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEY4193774.1"
                     /translation="MVADGRQRIDKWLFFSRAVKSRSLAAKLVVAGRVRINRDKAAQA
                     SDLVKPGDVLTLTLDRRISVWKIIGPGLRRGPAEEAKKLYEDMSPPPVPRKEAVAGAM
                     VPLRDAGSGRPTKKQRRQTDRLLDED"
     gene            complement(20153..23626)
                     /locus_tag="VGM46_14105"
     CDS             complement(20153..23626)
                     /locus_tag="VGM46_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEY4193775.1"
                     /translation="MNIQPKQTEPLILSGRDVTAVLGPTNTGKTHLAIERMVAHESGI
                     IGLPLRLLAREVYGRVCEKVGANKVALITGEEKIQPKGAKYSVCTVEATPRETDAAFV
                     ALDEVQLAGDLERGHIFTDRILHLRGRQETLLLGAATMQGILQKLLRGISVVTRPRLS
                     HLAYAGSKKLTRLPRRTAIVAFSADEVYAIAELIRRQQGGAAVVLGALSPRTRNAQVA
                     LYQSGDVDYLIATDAIGMGLNLDVDHVAFAQNRKFDGFQYRQLTAAELGQIAGRAGRH
                     LRDGTFGVTGQVDPFEEDLIQKIEGHDFEPVKVLQWRTTGFDFSSLDGLKRSMEANAP
                     VEGLTRALPAVDAQALEHLSRDEDIRALATGRERVALLWEACALPDYRRIAPAQHADL
                     IASIYMDLAKRGHVDENYMAEQVRRADTTEGDIDTLSHRIAQIRTWTFVSNRPGWLAD
                     QAHWQEKTREIEDRLSDALHERLTKRFVDRRTSVLMRRLRENTMPEAEISPAGTVLVE
                     GHHVGELQGFRFTADQSAGGEDAKAVKAAAHKALAAEFEARAERFGAAANGDIALGSD
                     GTLRWIGAPIGTLVAGEDALKPRLVLLADEQLNGPARDKVAARADRFVNFQVELLLKP
                     LVDLKNAEQISGIARGIAFQLVENFGLINRRDIAEEMKTLDQEGRAGLRRLGVRFGAY
                     HVFVPSLVKPAPAGLITLLWALKNDGKDKPGFGDVVHALASGRTSVIIDPAFDPAFYK
                     LAGYRNLGRRAVRIDILERLADLIRPATNWKPGIGPRPDGGYDGTAFMVTPAMMSILG
                     ANADDMEEILKGLGYRAEPKPATEVKARLEAQDNAAREAAQAKLAAVEAEAIEADATA
                     GLPVEEAAGGGEEEASGDVSASAGSDTGETPAVEPAVETAPAMTVAAETQPETPAEVA
                     EATLLAQEAEAIEADATAGLRLEEAGHADEKAEPSIDAGQAVAHAVAGAAETQAVDAA
                     AAATDEGATPGKVEAAGEPPKPILLWRQARFERPRGGHRQDNRQRGAQGRNNNVPATG
                     QPAAEQGDAAEKSQGKGRPRFDRSRYKGRPSEGGKPGEGGQSADRPQGEGAGRAYGKP
                     GGGGRPAQGGKPYREGGRPDNRPDNRNEGRGEGHGKPAFQSKPREERPVRFDPDSPFA
                     KLAALREQLKK"
     gene            complement(24284..25009)
                     /gene="phbB"
                     /locus_tag="VGM46_14110"
     CDS             complement(24284..25009)
                     /gene="phbB"
                     /locus_tag="VGM46_14110"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HEY4193776.1"
                     /translation="MTKAAIVTGGTRGIGAAISVGLKNAGYAVAANYAGNEEAAQRFS
                     AETGIKAYRWSVADYDACVAGVAQVEADLGPVSVLVNNAGITRDAMFHKMTPQQWKEV
                     IDTNLSGAFNMAHPLWNGMRERKFGRIVTISSINGQKGQAGQVNYSASKAGDIGFTKA
                     LAQEGARAGITVNVICPGYIGTDMVKAIDEKVLNERIIPQIPVGRLGEPEEIARCVVF
                     LASDDAGFITGSTLTANGGQYIA"
     gene            complement(25120..26307)
                     /locus_tag="VGM46_14115"
     CDS             complement(25120..26307)
                     /locus_tag="VGM46_14115"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEY4193777.1"
                     /translation="MSAANSIVVASAARTPVGSFNGAFAATPAHELGATVIRELLVRA
                     GVDAAEVNEVILGQVLTAAQGQNPARQASIIAGLPKETTAWGLNQVCGSGLRAIALGM
                     QQIAGGDADVIIAGGQESMSMSAHAQHLRAGTKMGDLKLIDTMIKDGLWDAFNGYHMG
                     TTAENVAKQFQITRDDQDRFALASQNKAEAAQKAGKFKDEIVAFTVKGRKGDTVVDQD
                     EYIRHGATMDAMQKLKPAFDKDGTVTAANASGINDGAAGVLLMSEAEAARRGITPLAR
                     IASWATAGVDPAIMGTGPIPASRRALEKAGWAVGDLDLVEANEAFAAQACAVNKDIGW
                     DPAMVNVNGGAIAIGHPIGASGARIFNTLVFEMKRRGARKGLATLCIGGGMGVAMCVE
                     AMN"
     gene            26596..27207
                     /gene="phaR"
                     /locus_tag="VGM46_14120"
     CDS             26596..27207
                     /gene="phaR"
                     /locus_tag="VGM46_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HEY4193778.1"
                     /translation="MAAKDEPVVIKKYANRRLYNTGTSTYVTLEDLAEMVKKGEEFTV
                     QDAKTGDDITHPVLTQIIFELENKDGQNMLPVPFLRQLIAFYGDQMQAIVPSFLEQSM
                     MAFSKEQSRFREQMKESLAQSPVEVMKSPIKALEEQTRRNMEMFQNAMRMFTPFPQAG
                     AGAAAEPAGATNQPSSDAPEKPGDLQELKEQIAAMQRKLDNMS"
     gene            27357..28424
                     /locus_tag="VGM46_14125"
     CDS             27357..28424
                     /locus_tag="VGM46_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEY4193779.1"
                     /translation="MTEPRLVPVFDGHNDTLLKLYLASDPDKEKLFIDGSPAAWHIDL
                     PRAKKGGLVGGMFAIFPPPVDERKHDAGRPAPMPGNDEPLPPQLSMNAARVSTLGMAS
                     ILFRLERAGALSICRSAADIRAAMARGSLAAVFHIEGVEAIDPELAMLDVLHAAGLRS
                     LGIVWSRPNAFGNGVPFRFPSSPDTGDGLTDAGKALVRACNRLKIQIDLSHLNEKGFR
                     DVAALSDAPLVATHSNVHSITASARNLTDWQLGAIRESKGMVGLNFATGFLNPAGPSG
                     RGADIAMMIRHVDAMLEALGEDGVGLGSDFDGAPVPSEIGDVSGLPRLIEAFEKHGYG
                     PELIEKIAWKNWIGALERSIG"
     gene            complement(28426..28674)
                     /locus_tag="VGM46_14130"
     CDS             complement(28426..28674)
                     /locus_tag="VGM46_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193780.1"
                     /translation="MAGEADDEAAEQWALVNTPLGEEWSGRTRYAAAMFFHKRGEIGD
                     GALEVYRICARLDREDPLPIIRDRGVGKDWLKRMGFAG"
     gene            complement(28678..>28813)
                     /locus_tag="VGM46_14135"
     CDS             complement(28678..>28813)
                     /locus_tag="VGM46_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyl transferase family protein"
                     /protein_id="HEY4193781.1"
                     /translation="VDDLWVEIYAMRELQYQKMMKRAKVVQRPFIKLDRRTANPPADF
                     "
BASE COUNT         5221 a         9544 c         8827 g         5221 t
ORIGIN      
        1 atttcgatgc aaacgctgct ggcgaaaacc ggagccgagc gtgtcgtcag ggcgatgccc
       61 aacgccgccg ccgaaattgg cctgtcctat acgccgtggt tctgctccgc cgccgtttcg
      121 caggccgacc gcgctttcgt gcagtcgctg tttgaaagct gcggcaaggc cgacgaggtc
      181 gcggcggagg cggaaatcga ttacctgacc gcgctcagcg gttccggccc ggcttttccg
      241 gcgctgcttg ccgacgccct gcttggccat gccctttcgc aaggcctgcc cgcggcgatt
      301 gcccggcgcg cggtggaggg cgtcgtctgc gacgccggcc ggttgatcgc ggcatccgat
      361 gccacgccgg gcgagatggt gcgcaccttc atggaatatc gcggcaccac cgatgccggc
      421 ctgcgcgcga tgaagaacgc cggatttgcc gaggcgattc acgccggcct gtcggccgcc
      481 gcagccaagg ccgtggcgat ggaaaaccac ggaaacagga gtgaggaata gccattcggc
      541 aagacggtgt gccggatggc tggatttgtg acaacaccgc gaaaaaaggg aacaaaatga
      601 agaaacttct cgcatcgacg tgcctgacaa tgggattgct gacgatggcc tcgtttgcct
      661 cggcggcgga gtgcggcaac gtcaccatcg cctcgatgag ctggcaaagc gccgaggtca
      721 tgtccaatgt cgacaaggtc atccttgaaa aaggctatgg ctgcaacgcc gagatcatcg
      781 tcggcgatac cgtgccgacg atcacctcca tggtcgagaa aggccagccg gacgtggcgc
      841 cggaagcctg gatcgacctg ctgccggagg tcgtcgaccg tggcatgaag gaaggcaaga
      901 tcgtcaccgc cgcgacctcg ctgccggatg gtggcgtcca gggctggtgg atcccgaaat
      961 atctggccga cgcccatcct gacatcaaga cgattgccga cgcgctgaag caccccgacc
     1021 ttttccccgc gccggaagac gccagcaagg gcgctatcct gaatggcccg gcgggctggg
     1081 gcggcactat cgtcacctcg cagctgttca aggcattcga cggcgaaaag gccggcttca
     1141 cgctggtcga caccggttcg gctgccggcc tcgacggtgc catcgccaaa tcctacgagc
     1201 gcaaacaggg cttcgccgcc ttctactggg cgccgaccgc gctgctcggc aaatatgaga
     1261 tggtcaagct cgacaatggc taccccgtcg acaaggccga atgggcgcgc tgcaacaccg
     1321 tcgccgactg ccccgatccg aaggtcaatg gctggccgaa ggaccgcgtc ttcacgctgg
     1381 tcaccaagcc gttcgccgac cgtgccggac cgcaggtgat ggactatctc accaagcgct
     1441 catggtcgaa cgacaccgtc aacaagttga tggcctggat gaccgacaac cagtccaccg
     1501 gcgccgatgg cgccaaccat ttcctcaagg aaaaccccga tctgtgggcc aagtgggttt
     1561 cgccggaagc agcggagaag atcaaggccg gcctctgatt ttcctgttcg ctcgacagaa
     1621 aatggcagcc gatgcgctgc catttttccg ccgcaggcac gccgcgcatc caccacccgt
     1681 catacttggg ctttgcccga ggatgacggc gagcagacat ctatcgacaa tgcgaaaagc
     1741 aaaccggcac cgcagcccac aacggataat ctacacgcat cctcgcctta taattcaggg
     1801 gctttcgttc gcttcctgcc gcaatgtcgc gatgaagcgc ttggttcgct cgcgtttcgg
     1861 attgccgaac aactcgccgg ccggccccgt ctcgacgacg acgccggcgt cgaggaacac
     1921 cacttcgcgg gcgatcttgg aggccaggcg caaatcgtgc gtcgccatta ccatggtggt
     1981 gccttcgccg gccagctggc caagcacgtc gaccacctcc tgcgccagtt cggggtcgag
     2041 cgccgaggtc ggctcgtcgc acaacagcac cttgggcgac ggcgccagcg cccgcgcgat
     2101 ggccacgcgc tgctgctggc cgcccgaaag ggtcgccggc caggcgtcgg ccttgtgctc
     2161 catgccgacc ttttccagca gttccatcgc ccgcttcttg gcgcgctcgg ccggccattt
     2221 cagcacggtc gtcagccctt ccatgacgtt ctcgatggcg gtgcgatggg gaaacagctg
     2281 aaaactctgg aacaccatgc cggtctgccg gcgcacgcgc tggatatcct tggtcgcaac
     2341 cttcacgccc ggcttgaacg tcaacgcctc gtcgccgatg cgcaccgtgc ccgaggtcgg
     2401 gatttccagc aggttgatgc accgcaaaag cgtgctcttg ccgccgcccg aaggaccgac
     2461 aagcgccgtc acgctgccct ccgcgatggt gacgccgacg cccttcagca catggttttc
     2521 gccgaagcgt ttttcgatgt cggtcagttc gatcatgagc gcgcctccag gaaaccgcca
     2581 tagcggtcta gccgcacttc cagccgcgtc tgcaacgccg acagcaccga actcatggcc
     2641 agatagagaa tcgccgcctc gacataaagg atcagcggct cataggtgac ggcgacgatg
     2701 cgctgcgccg cctggaacat ttccggcacc gtgatggcgg cggcaagcga agtatccttg
     2761 accagcgaga tgaacgtgtt ggagagcggc ggcacggcca cgcgccctgc ctgcggcagg
     2821 atggtgcgcc gcatcgcctg gctccagctc atcccgatcg aataggcggc ctcccattgg
     2881 ccccttgcca ccgagccgat ggccgcccgg atgatctccg acgtataggc gccgacattg
     2941 agggtgaagc cgatcagcgc cgccgtaaag gcatcaagca ggatgccggc ggagggcagc
     3001 ccgtagaaga tcaggaacag ctgcaccaga agcggcgtgc cgcgaaatat ccagacatag
     3061 aaacgcacca ccgccaccag cggcttcggc ccgaacagcc ggatcagcgc gacgaccagc
     3121 ccgacgccga ggccgaaagc gaaagacaga agcgtcagcg gcgtggtgaa aacggctgcg
     3181 gcccataaaa gggggccaag cgaatccagc atcaattgca gccagtgcgg cacggaatca
     3241 tctcctttgc ggatcgccgg aacgaaccgg cgcgccatat catgcgaaaa acggcgagcc
     3301 gtcaaagacg gcccgccgca ttgtcgcgtc aagccgggcg tacgatcgcc cggcctgcaa
     3361 gcttacttcg aaacgtcctg gccgaaatat ttgtcggcga tcttctgata ggtgccgtcg
     3421 gccttgatgt cggccagcgc cttgttgatg gcggcgacca gttccgggtc gccctgacgc
     3481 acgatgatgc cggaataatc ggcgttttct tcctgcgcgg cgatcttcac atcggcgtcg
     3541 ggcttgtgct tcttgaagtc gaggtaggac aggctgtcat tgatggtggc gtcggcgcgg
     3601 cccgtcagca gaagctggat cgactggtcg aaaccatcgg tgccgaccag ttcggccccg
     3661 ttcttttcag ccagcttgcc gaaattcgag gtcagcgact gcgccgactt cttgcccttc
     3721 aggtcggcaa agctcttgat gtcggcattg tcgcccttga cgatcagcac cgccttcgac
     3781 gcgatgtagg gatcggagaa ggcatatttc gccttgcgct cctcggtgat gccgacctgg
     3841 ttgatgaccg cgtcatagcg cttgccgtca aggccggcga tcagcccgtc ccacttgcct
     3901 tcgaggaaat caggcttgac gccgagcttg gcggcgaccg cctcgccgat ctcgacgtcg
     3961 aagccgacca gcttgttgtt ctcgtcatga taggtgaagg gggcgtaggt gccttccgtg
     4021 ccgatcttga tcgcgccatc ggccttgatc tggtcgaggt tggcgccggc gaacgaagcg
     4081 gtgatggcca gcgcctgcac ggcgccggcg acgatgagtg aattaagcca tttcatggtg
     4141 gggaaatcct gattttgcgc ggatgacacc gacgcttgtg accggcggca atctgacaga
     4201 gcggctccgc cagattaagg aaatttattc caatttttcg ccattacaag aatcaaattc
     4261 tgatttcccc ccgttcaatg cctacaccca tcattccggc cttcagattg ccgggaacca
     4321 tttttcgcgc aagcccgtta tcgccacgcg agcgtgacat ggcgggattt caagccgccg
     4381 tccggacgcc ccttggaaca ggccgaaagc gggaagggca ggcaaaatga tgcaggacag
     4441 cgccgggcgc agtttcatcc gcagcgccat gcaggccccc taccttgagc gcgacgagga
     4501 acatttgctg gcgcagcgct ggcgcgatga cgccgaccag aacgccttgc accgcatgac
     4561 cgtggcccat atgcgcctag tcatctcgat ggccatgaaa ttccgccatt tcggcttgcc
     4621 cgccggagat ttgatccagg aaggccatgt cgggctgctg gaagccgccg cccgcttcga
     4681 acccaaccgc gaggtacgct tttcgaccta cgcgacatgg tggatacgcg cctcgatgca
     4741 agactacatc ctgcgcaact ggtccatcgt gcgcggcggc accagttcag cgcaaaaggc
     4801 cctgttcttc aatctgcgcc gcttgcgcgc ccgccttgcg gccggctccg ggccgctcgg
     4861 caacgagatg ctctaccggc agatctcgac cgcgctcggc gtttccgagg ccgatgtcgc
     4921 cacgatggat tccaggcttt cggcgcccga cagttcgctc aacgcaccgc tgaccgacga
     4981 tgccggcgct gtcgaacgca tggatttcct ggtcgccgac gatcccttgc ccgacgaggt
     5041 cgtcggcgac acgatcgacg tcgaacgccg tgccgtctgg ctcaaatcgg ccctaaccgc
     5101 gctcgatgcg cgcgaactca ggatcatcga ggagcgccgg ctgaccgatg agggcgcgac
     5161 gctggaggcg ctgggcgaaa agcttggcat ctccaaggag cgcgtgcgcc agatcgaggt
     5221 ccgtgccatg caaaagctga agacggcgct ggtccggcag aacccggaat tcctcgcggc
     5281 ttagagtatt tccagcaaag tgtgaaacga tcttgcgtcc ggaaatgcag tcaaaaaaaa
     5341 gatagagcga gtttgtcgat tcagcaaaaa tcgaaaccgc tccagcgctg ttgccgccct
     5401 atttgtcggt gacgatcttg accttgtcgc cggcggaaac gctggcgccc ggcgacatgg
     5461 cgttcagcac caggaacagg tcgagcttgc ggtcgacgcc gatcatcatc gccgacagtg
     5521 aaccgaccgt ttgccccggc tgcacggtga cgacgcggat atgcagcggc tttagcgcgg
     5581 ctttctgggc ggcatccagc acgcggaaac tgccgctcac cgaccgcgcc accgtatcca
     5641 gcgaggtgct ggcgatgggc gccgccgtca gcagccggta gacctgtccg ttggcccgaa
     5701 tgaccgcgat atcgaaccgc cagccatcgg cgctggcctt ggcggtcgcc gcctcattcc
     5761 cggcgatggt ttcctcatgc acgctggcat tgtcgaggcc ggccacccat ccgctgcgga
     5821 tgtaatcggg cagcgagcgg ttctggtcga ttgcaacgcc atcgaagcgg atcgccatgt
     5881 cgccggggcc ggtcgcggtg acggcggcgg cggaattgtc gatgatgaag ccgtccggca
     5941 ccgagaacga tatgccaagg cgcggatgca tgaaggtgtt gccgcgcaca tagccttcat
     6001 ccggcgtgtc gccgtagagc aggccgtcta tgccggcgag gaaggaattg cggtcgacgg
     6061 tgccgacccc cggctggccg aactcgcgcg catggcgctg cgccagttcg atgcgctgcg
     6121 gcgtattggg gtgcgtcgcc aggaagtcga ggctggcgtc ggtcgcgccg ctgatcgagc
     6181 ggaagtcgct ataggccgac atcgattgca ggaaacggcc cgccgcatag gggtcgtagc
     6241 cggcttcgcc caccgatttt atgccgatgg cgtccgcttc cagctcctgg ttgcgcgaaa
     6301 actgcgccat ccgaagcttg ccgcgaatga gcgccgcctt ggcggtcggg ctgtcgccta
     6361 gcacgtcgga gacgaccttg gtcgccagca cttcctcggc ttccttctgc tggcgctgca
     6421 agccgtgatt ggcggtgacg tggcccattt catgggcgat gacggcggcc agctcggccg
     6481 aatcattggc cagcgccaga aggccgcgcg taatatagag gtaccccccc ggcagcgcga
     6541 aggcgttgac gttgggcgaa ttcaggatgg tgatgcggta ggtctggctg gggttggccg
     6601 acacggtggt caggctgccg accaccttgg ctaccatgcg ctccagcttg gggtcggaat
     6661 attcgccgcc ataagtggcc agaatgcgcg gatgctgcgc cttggccagt tcggcaagct
     6721 tgctgttggc cgcgacatta tcgacggtga ccggccggtt ggacggttgg aagcccgatt
     6781 cctggatatc gggcgtgacc gcctggcatc cggccagcgc cccggtcagc gccaagacaa
     6841 agccggccag ggcgagatag cgcgccagcg gattcacttt cagtgcatgg gaaccggtcg
     6901 acagatcggt ttcctttcga gttcgagcat acacgttcgc gaaaactctc gcattcagga
     6961 tgttgcttta gcgcaatcgc ggcgagaatc gaaacattta gccggaacta gccacactca
     7021 atcctgcatg gcaagcaaac gccatagcgg accggacggt cttgatcgta aattatgtcc
     7081 gctttcaggc aatgtcgcgt gatcggccgc cggccagttg cgatcccata tagcgcctga
     7141 acacttcggg tcccgaccct tcgaagaaat cggcggcgga accggcggca gcgaccctgc
     7201 cattctccag aaacaccatg tcgcgcgcta tgcggcgcgc atcctccggc tggtgggtga
     7261 cgaacagcac cgtcatggcg cgctcggcct gcaaaccggc gacaaggtcg agcatgtctt
     7321 cgcgcaacgc cggcccgagc gaggcgaacg gttcgtccag cagcagcacc gggcgttcgc
     7381 gcaccaatac gcgggcaagc gccacacgct ggcgctcgcc gccggacagt tctcgcggca
     7441 gccgcctttc cttgcccgaa agcccggtgc gggcaagcgc atcgataatg gctgcgcggt
     7501 cggcggcgcc aagcttcagg ccaggcgagc ggccaagccc gacattctgc tcgacgctca
     7561 aatgggcaaa cagattgttt tcctgaaaga ccatcgagac gggccgcatc gagggctggt
     7621 cggcggtaac ttccttttgc ccgatcagca cccggccggc gcgcggcgtc tcgaaaccgg
     7681 cgatcagatt gagcagcgtc gattttcctg aaccgctcgg ccccatgatc gcggtaatgg
     7741 tcgccggagc gaatgcgacg tcgaaggaaa gcgcagtctc gccatagctg aacgacacct
     7801 cgtcgaggcg tacgccccaa gcttcctttt ccatgccggt attcatgacg gccgatcctt
     7861 tccaaggcga tccgcagcca acattaaggc aaggcagacg aggccgagca acaaggccag
     7921 ccccgccgca tcggcggtac ggtagctgcc catccgcgcc agcagaagat agggcagcgt
     7981 ctgcacggaa tcgccgccga acagcgcgat gacgccgaga tcgcccagcg aaagcgccat
     8041 ggcaaaggcg aaggcagtgg cgaacggacg gcgcagggcc ggccagtcga ccagacgcaa
     8101 ccgattccag cccgcaatgc caagcagcgc gcacagccgc tcatggcgtt cgctcgccgc
     8161 atcgtaggcg ggacggatgg cgcggacggc aaacggcatc gccatcacgg cattaacggt
     8221 tacgaccatg accggcgcga tggcgaacac atcggcgaaa cggcgaagca gcaggaacca
     8281 gcccgcgccg ataatgatcg gcggcaccac cagcacgaag cccgcgccgg tatcggtggc
     8341 atattcgagc gcgcccttga tgccgccgcc ccgccccagc gtcagtgccc gccgcgccgc
     8401 caccagcgaa aacgacagca ttgccgacag caacgccgac agcacggcca gcacgatgct
     8461 ggtcagcgtc gcccgccgta ccgcgccttc tgcggcaagc cgggtgaggt cggcgtcaag
     8521 ccccgccatc accgtcgcgg ccatcggccc ggcgacgaac agcagcgcca gggcaatgag
     8581 cgtaatgtcc aatgccgtct ccggcctgcc tggcgtcaga aaccggcgcg gcgcgaccgg
     8641 cagattggcg tcgcccgtgg tgttggcccc aagccgcgcc agcgccagca cgaccgcgaa
     8701 ggtgagcgcc acctgcaaag cggtcagcgc caccgcccgc gacggatcga aatcgaagcg
     8761 caacgcctga tagatggcga cttcaagcgt ggtggcgcgc ggcccgccac ccagtgtcag
     8821 cacgatggtg aaggaggtta cgcacagcat gaagacaagg ccggcaacgc ccggcagcgc
     8881 ctggcgcagc gccggccatt cgatcagcct gaacgtggcg cgcggcccca tgccaagctg
     8941 gccggccagc cgccactggt cggccggcac cgtctgcaac gcttcgagga acagccgcac
     9001 gccaagcggc aggttgaaga agacatgggc gaccaatatg ccggaaagcc cgtagatgcc
     9061 gggccatccc tcgccgccga ttgccgaaaa aataccggcg aaataaccgg cgcgcccata
     9121 gagcgacaag acgccaagcg ccgccacgat cgccggcagc gccagcggca cggcgaacaa
     9181 ttgcagaata agactgcgcc cgaaaaaacg cggatgccgc gacagcgccc gcgccaccag
     9241 caaagccggc gcgaccgaca ggacggtcga cagccccgcc tgccggagcg tgaagcgggc
     9301 aaccctgaac agatagccgt caaaggccga aacggccccg gaaatatcct gcccgccctg
     9361 cacaacaagc ccggcaaacg cgccgccgac aagcagcacg acaaaggcaa gcgcgataat
     9421 gccggcggcg atgcgcgggt ccgccttgcg gggagagccg gcaagaagat gccgtgaagc
     9481 ggcgtccgcc agccggggtg aaggatcgat caagcccatg gcccccacct tgccgcgatc
     9541 gcttcgcagc gcaaggcgtt gccgtttccc ccgccctttg agggcagggt aaggcccgct
     9601 ttcatttgct catcacggcc agccattcgt ccacccaggc cttgcggttc ttggcgacct
     9661 cgtcggcgga aaacagcagc gtctttgccg gctttacaag cttgtcgaac gccggatcga
     9721 gcggcttgtc ggttttgccg gccggaaaca tccagttggt ctcgggaatg gcgtcctgga
     9781 attgcggccc ggtcatgaag gcgatgaatt tttccgaaag cgggttcttg gccccaacgg
     9841 tagtggtgcc ggcaacctcg atctgaagat aatgcccctc gctgaacgac gccgcctgat
     9901 agcggtcggt tttctccgcg atcatgtggt aggccggcga agtggtgtag gaaaacacca
     9961 tcggcgcctc gcccttggtg aacaggccgt aggcctcgct ccagccgggc gtcaccgtca
    10021 gcacgcggtc cttcagcctg gcccaggctt ccggcgcctt gtcgccatag accgacttta
    10081 cccacaaaag caggcccagc cccggcgtcg aggtgcgcgg atcctggatg gcgatcttct
    10141 gcgacggatc gccctcgacc agttccttca ggcttttcgg cgggttcttc atcgcctgcg
    10201 tatcgtagac cacggcgaaa tagccgtagt cgtatggaac gaaaatatcg tccgtccagc
    10261 cgcccggcac ggacgtctca gccacggagc catgcggcgc aaacaggccc gtcgccttgg
    10321 cttccgccgt cagattggtg tcgaggccga gcaccacgtc ggccttggtc gaagcgcctt
    10381 ccagcttgac gcggttgagc agcgccacac catcggcgac cgagacgaaa tcgacctcgc
    10441 agccgcactc ggcctcgaac gccttcttca cctgcgggcc tggtccccat tcggcggtga
    10501 agctctcata ggtatagacg gtgagttttt ccttggccgc cgccggaacg gcaagcaagg
    10561 cgatggccgg gataagggag tagaaaaatg tgcgcatcgg cgcctccttc gcaagtgttg
    10621 aaaggaattg aggcgtcggg ctaccgaggc atctaatccc tccgccggta caaaccggat
    10681 caggttcagc gggttggcga gatcacctct cagccggtcc acgaaaatcg cgtccggcac
    10741 cccgttagag cgcctcgaca cataagaccg cttggggagc gaggcaagcg gaagtttgcc
    10801 catggtgtgg tcacggcctt ccgtttccgc cgccgggctg atagaggcga cgcccatgag
    10861 ccaattcacc attctgcttg gcggcgatct cgttcacacg ccgctgcttg cccggcaggt
    10921 cgccggcacc cgcaccatcg ccgccgatgc cggcatcggc catgccgcca tgctcggcct
    10981 gatgcctgaa ctatgggtcg gcgatttcga ttccgtgccg ccgaacctgc ccgcccatct
    11041 ggcctccgtc ccgcgccaga cattcccgcg cgaaaaggac aagaccgacg gcgaactggc
    11101 gatcgcgact gcattgcagc gtggcgcgac ctcgctgatc cttgccggcg ccttcggcgg
    11161 cgcgcgcacg gaccacgcct tcctccacct ggcgctggcg gtacatcttg ccgaagccgg
    11221 aaccgaggtg cttttgacca gcggcgcgca ggaaggcatt ccggtgctgc gcggtgaacg
    11281 atccttcgac tatgccgatg gcaccttgtt ttcgatcctc ggcttctccg acctttccgc
    11341 cttgaccgtc gagggcgcca agtggccatt ggacaatgtc gaggtcgctt tcggctcctc
    11401 gctgaccatc tccaacgaag tcaggggccg cctgcgcgtc gagcttggcg acggccgcgc
    11461 catgctgctg gcccatccct ttcccgtcgc ggggagctag gcaatggccc cgccacttct
    11521 caatctcgac gctataaagc tgaccttcgg cggcacgccg ctgctggacg gcgcggaact
    11581 gaccgcctcc gccggcgaca agatcgcctt ggtaggacgt aacggttccg gcaaatcgac
    11641 gctgctgaag atcgccgccg ggctggtcga gccgcaggat ggcgaggtct tccgccagcc
    11701 ttcggcaacc atccgctatt tgccgcaggc gcccgacatg gaaggtttcg ccaccgtgcg
    11761 cacctatgtc gaggcaggcc ttggcccggc cgacgacccg caccgcgcca cctatctgat
    11821 ggagcatctc ggcctgagcg gcgaagagcg ccccgccgac ctttccggcg gcgaagcgcg
    11881 ccgcgccgcc cttgcccgcg tcatggcgcc ggaacccgat attttgctgc tggacgagcc
    11941 gaccaaccat ctcgacctcg gcgtcatcga atggctggaa gaagaactgg tgcgcacctc
    12001 ttccgcgctg atcgtcattt cgcacgaccg ccgtttcctc gaacgcgtct cgcgcgccac
    12061 cgtctggctg gaccgcggcc agacacggcg gctggacaag ggttttggtc atttcgaggc
    12121 gtggcgcgac caggtgctgg aagaagaaga gcgcgaacag cacaagcttg gccgccagat
    12181 tgtacgcgag gagcactggc tgcgctatgg cgtgaccgcg cggcgcaagc gcaacatgcg
    12241 ccgccttggc gaactgcaaa cgatgcgcca gcagtttcgc ggccatcgcg gcgccgaagg
    12301 cagcgccacc atggttgcca gcgacgccac cgaatccggc aagctggtca tagaggcccg
    12361 caacatcggc aagagctttg gcgatctcac cgtggtcgac ggtttttcga cgcgcatcca
    12421 gcgcggcgac cgtgtcggcc tcgtcgggcc gaatggagcc ggcaagacaa cgctgctgaa
    12481 aatgctgacc ggcgaactga agcccgacgc cggcagcgtt cgcctcggca ccaacctgga
    12541 aatcgccacg ctcgaccaga agcgcgaggc ggtggacccg gccgaaacgc tggcgcaata
    12601 tctgacggat ggacgcggcg agaacctcgt catcaatggc gagcagcgcc atgtcgtctc
    12661 ctacatgaag gacttcctgt tcaaggccga gcaggcgcgc actccggtgc gcgaactgtc
    12721 gggcggcgag cgggcgcggc tcatcctggc gcgcgttctg gcgcggcccg ccaaccttct
    12781 ggtgctggac gaaccgacca acgacctcga catggaaacg ctggaactgc tgcaagagct
    12841 ggtcgcgggc tttgccggca cggtcatcct ggtaagtcac gaccgtgatt tcctcgaccg
    12901 caccgtcacc agcgtcattg ctccggatgg caatggcagc tggatcgaat atgccggcgg
    12961 ctattccgat atgctggcgc agcgcggcgt gacccggctg gaaggccgca aggcgcgcgt
    13021 gaaggccgat gacgccgcca cagccaacag agcggaaccg tcagcgccta aagcgccttc
    13081 gaagaagctg tccttcaagc agaaattcgc gctggaaacc ctgcccaaga agatcgaggc
    13141 ggtgacgcaa tcgatcgcaa ggcttgaaaa caacatcgcc gatcccgcct attacgaacg
    13201 cgacccggcc tcgttccaga aaaccatcgc cgccctcgac aaggagcgca cgacgctggc
    13261 cgcgctggag gaagagtggc tggaactgga aatgctgcgc gaagaacttg aggggtgagg
    13321 cgccggttcg cccttcgggc gaattgaaag gcccggtggg cctttcaaag gcgccgaacg
    13381 cccgaggcca tagccgaggg ccggaccaag tcgtgtgacg ggatgcggcg gcacaccagg
    13441 ccgggccggt tcatccaagc gccatcaacc gaattacttg ctaacgcttc aacatgcgca
    13501 tattctatgc gcagttttat tggagatctt gcaatgcttg gcaaacgtca gtccccgcaa
    13561 cgaaaaccga caaacctttc cctcgatgca acgctcgtcg ctgaggcaaa ggacctcaac
    13621 atcaacatct cccgcattgc cgaagacggt attgccaggg ctgtctcaga ggaaaaggcg
    13681 cgtctttggc ggatcgaaaa ccgggattcc atccaaagct tgaatgccta tgttgaggaa
    13741 catgggatac catttgagaa cgaccgccag ttctgatggc caggtacgat gttttctcaa
    13801 atcctaccgg accgggctac ttgctcgaag tccaggccga cctcctggag gatttgaata
    13861 cgcgggtcgt cgtgccgctg gtgccgcatg acgtcaagtt aaaggccgtt cgtaggctca
    13921 atccagtctt catgatcgaa ggcagacagt atgtcctgtt tacccatctg atcgccacca
    13981 tcccggcggt gcgcctcact ggacccagaa cgaacctgat tcgccaccac gacgagatcg
    14041 tcaccgcgct cgacatgctg ttccagggct tctgagcgaa agccggcaga aagccggcct
    14101 caccccgccg tcttggcctg ccaggccttc atcgcgtcat cgaacacctt ctcgccggga
    14161 atgccctttt ccatggtcaa aagggcgcgg cggccggatt tatagatcac cggaatatcg
    14221 atccagccct ggttcctcaa cagcgtgagg ttggtctgct cgtcggcctt ggtgtcgttg
    14281 agcgccacca ggaagaaacc gtctgcgatc ttcgccggga tgccgaccag cgggctgccc
    14341 gcatcctgtt ccgccggctt catcgccacg cgtaatatat tgtcgatgcc gccgccttcg
    14401 aagccgtccg gcgtcaggaa gataagctcg ataatatggc tggcgggcag cgtctcgtcg
    14461 gtgttgcggt ggatggtcat gcgcagctgc acgtccttgc ccggaatggt cacctcggcg
    14521 cgtatcgccg gttccggcgg cgcgtcgccg cccggcgatt cctgcaccag cgaccagacg
    14581 atattgccgg actcggcgga gccttgcgca gcgctggtgc gctcctcata gaagatcgct
    14641 ttctggccga ccgcgagcgc cgattgcgct ggcgcagccg caggcgcggc cgcaggcgcg
    14701 gccgtcgtcc ctgccgccgg agcggtcact ccgcccacag ccgccgcggc tcccgccggc
    14761 ggcgcagccc cctgcgcggg cggcggcgtc gtcaggccga ccaccgatgt gccctcgcca
    14821 atcgtattct cgccgccggc cggacccgga tcggtctcct tgccctcgga tgtcaggcgc
    14881 tgcgtgaatt tgggcgcttc gccagtatcg gtggcggtat ttgccggcgt tgcctgcccc
    14941 ggctgtgtcg tcggcgctgt cggcgcggca ggcttggctg cggccagcgg ctcttcctta
    15001 accacttcgc cgccggcctt gcctgcgccg aacatcgact tgaacgcgcc cgcgttcagc
    15061 cagatgccat aagcaccgcc ggcaaccacc agcagcgcca cgacagccgc aaccagcccg
    15121 ccccgcccgc gcgaacgctc gctggagccg gccggttcaa aaacatccgc ttctggatca
    15181 gtgtcggggt agggcgaaaa cgcctcgtcg cgaacggctg gcctagcttc ggcaggccgc
    15241 gccgcttccc gatcagtgcg cttttccgca gccggggcaa agcttggcgc gggggccggc
    15301 gtcggaaccg gttccggcgc tttgcgggtc tccggcacag gcgcctgcca ggccggctct
    15361 atcctcggcg ccggccgcag atggctgggc tggttcttgt tgcggtcgat ggaagagaag
    15421 atattttcca gttccgcgag cgggtcggaa gccggcgcag gtgccggcgc gggcttggca
    15481 tagccctttt cgaccgtggc gatggcttct tccagcgtgc gcttctggcg ctcggccacc
    15541 gcttcgggcg gcggcggcac gatggcggca agcttggctg cgatggtcga gcgcgccttg
    15601 tcatagaccc tggcgcgcac gtccggcgtc gtatcgccca tgccgtcgat ggtctttttc
    15661 aaaacagcaa cgaaatctgc catgcttcag tagacctgta tctttctccc cggtcggtcc
    15721 ccgcaaatca gctgcgatgc ccggagaaga acctgattaa tcttgaaacg gatcggtcac
    15781 aaggatcgta tcgtcccgtt ccgggctggt ggaaagcaat gccacgggcg caccgatcag
    15841 ttcttcgatg tagcgcacat atttgacggc ctgcgccggc aggtcgttcc agctcctggc
    15901 gccggcggtg gtgcccttcc agccctccag cgtctcgtag accgcctcga cccgcgcctg
    15961 cgcgccctgg cttgcgggca ggtagtctat cgtctcgccg tcgagccgat aggccgtgca
    16021 gaccttgatc tcgtccagcc cgtccagcac gtccagcttg gtcagcgcga tgcccttgat
    16081 accgttgacg gcaaccgcct ggcgcaccag aaccgcatcg aaccagccgc agcggcgctt
    16141 gcggccggtg acgacgccga actcgtggcc cttggtgccg agaaattcgc cgatctcgtt
    16201 cgtctgttcg gtcggaaacg gaccttctcc gacccgcgtg gtgtaggctt tggtgatgcc
    16261 tagcacgtaa ttgattgcgc cgggaccaac gcccgaaccg gcagcagcct gccccgcgac
    16321 cgtgttggac gaagtgacga acggataggt gccgtggtcg atgtcgagca tcgtgccttg
    16381 cgcgccttcg aacaggatgc gctcgccggc gcggcggcgg tcgtcgagca ccttccacac
    16441 acggtcgaca taaggcagga tctggtccgc cactgaggtc aattcgccaa ggatcgcttc
    16501 gcgcgacact tcctcatggc caagcccgcg gcgcagcgca ttatgatgcg ccaaaagccg
    16561 gtcgaccttc aggggaagcg tttccaaatc cgccaaatcc atcaccttga ccgcgcgccg
    16621 gccaaccttg tcctcatagg ccggaccgat gccgcggcgg gtcgtgccta tcttggtccc
    16681 ggaactggag gcggcgtcct cgcgaaaacc gtccagttcc cggtgcaggg aaaggatcag
    16741 cgccgtgttt tcggctattt tcaggcgatc cggcgtaact tcgacgcctt gcgccttcaa
    16801 tttcgccatc tcggcgacga aagcatgcgg atcgaagacg acgccgttgc cgatgatgga
    16861 aagcttgccc ttgcgcacca cgcccgacgg cagcagcgac agcttgtaga cggtgccgtc
    16921 gacgaccagc gtgtggccgg cattatgacc gccctggaac cgcacgacga catcggcgcg
    16981 ctccgaaagc cagtcgacga tcttgccctt gccctcgtcg ccccactgcg aaccgacgac
    17041 caccacattg gccatagtgt cttcccttca attcctgcaa agaagctgcg gctggcgccg
    17101 cgaaacgaca ggcctctata acgtcaagac atggcaagcg cgaccattct ttgccgtgta
    17161 aaccacgccg aggcacaaga aatttcgctt ttcgcggcat ttacttggcc tgcggaccgc
    17221 cataagccga caaccctcaa tcaggtcgca ctccccatgc ataaaaaagc ctatctgctg
    17281 ctgctggcga ccacgctgtt ttgggccggc aataccgtgg ctggaaaact ggcggtcggc
    17341 cacatatcgc cgatgctgct gaacgccgcc cgctggagct tcatgctggc ggcgctgctg
    17401 gtattcggcc gcaggcacct ggcgcgcgac tgggccacga tgcgccggca ttggccgttc
    17461 ctgctctttg cgggattcat cggcttcacc ctgttcagcg tcgccatgta cagcgcgctt
    17521 ctctatacct cggccaccaa tgtcagcatc gaacagggcg gcattccgtt gtttgtcttc
    17581 gtcgcgtcct atctgttgtt tggagtgcgc accaccgccg ggcaggctgc cggctttgcg
    17641 ctttccttcg ccggcgtcgt ggtgacggcc acccatggcg aaattcacag cctgctgcat
    17701 ctcggcatga attacggcga tgcgctgatg ttgctggcca tcatcgcctt cggtttctac
    17761 acggcgctgg tgcggcgcaa gcccgacatc cactggatca gcctgatggc ggcgctgtgc
    17821 gtgggcgcgg tcgtcagcgc aattcccttc gtcgccctcg aagccgccag cggcacgctg
    17881 gtcccgcccg acggccaggg ctggctggtc gtcttctatg ccgtggtctt cccttcgctg
    17941 gccggccagg ccttctatgt ccgcgcggtg gaaatgatcg gcgccaaccg ggccggcatc
    18001 ttcgtcaatt tcctgcccgt ctggggcgcg ctgctggccg tggccatact gggcgaggaa
    18061 ttccacgcct atcatgccat cgcgcttttc atcgtcatgg ccggcgtatt tttggccgaa
    18121 tttagcggcc accgggcgca atccgcccgc atctgagcga cgaaggcgga gcgtccccag
    18181 cctagatgga cggttgcggg cgcgccccaa gaaaaaagcc cggcgcgagg ccgggctttg
    18241 catgaagatc gatgctttcg aggtgtcagg cagcttcttc ctgctcggct tcctcgttgt
    18301 cggccttggc gccgcgtttc ggacccttgt tgaggttcac ctcgatgagg cgaaccgctt
    18361 cggtttccga catgcggttg acggcggcga tttcgcgccc catccggtcg agcgccgctt
    18421 cataaagctg gcgctcggaa taggactgct ccggctggtt gtcggcgcgg tagagatcgc
    18481 gcaccacttc cgagatcgag ataaggtcgc cggaattgat cttcgcatca tattcctgcg
    18541 cgcggcgcga ccacatggtg cgcttgacgc gggcgcggcc ctgaacgacc ttcagcgcgc
    18601 gctcgacata atcttcctcc gacagcttgc gcataccgat ggaggtcgcc ttggcgaccg
    18661 gcaccttcag ccgcatcttg tccttctgga agtcgatgac gaaaagttcg agcttgtgcc
    18721 cggccacttc ctgctcgtcg atcgagacga tctggccgac gccatgcgcc ggataaacaa
    18781 tgaactcgcc ggttttgaaa ccgtttcgcg ccgacgtgga aaccttcttc tgggggatcg
    18841 ccgttgccat tacgcctgaa ctccttcgtt gtagtgtcgg ccgttgtagt gtcggcaaac
    18901 cgccgaccga acccgaacga ccgtgattga cccggccttg ccgccagtcg caaaccaacg
    18961 ccccgaaaga aggaacagag ccggcaagcc gctgtcgcgc gatcgccagg cccagattgc
    19021 tccggtccgg ttcgtagaat tcacctttca gtgatttagg cgtccgccgc catagatcga
    19081 cttcgtgtct ggcatattca ccgtagataa cacaaaaagt cggaagaatc aagaatttgc
    19141 tgcatgcgcg aaggcacagc gccaccgccg ccgggcaaaa tgaccgtcat tgccggccgg
    19201 cggcacctga agcttagttg ccctcgccgg gttcggcgga aaaatacttc tccagctttc
    19261 cggtttcgcc gtcgaagctt ttggcgtcgg ctggcgcatc cttcttggcg gaaatattgg
    19321 gccatttgtc ggcatattcg gaattgagtt gcagccactt gtcgaggccg gattccgtat
    19381 ccggcttgat cgcgtcggcg gggcattccg gttcgcagac gccgcaatcg atgcactcgt
    19441 ccgggtggat gacgagcatg ttctcgcctt catagaaaca gtcgaccgga cagacctcta
    19501 cacagtccat gtacttgcat ttgatacagt tgtcggtaac gacataggtc atcggtcggc
    19561 tccgggaaat cccgtattgg caggctttcg cccgcactgg caggctttcg gggtcaggta
    19621 acccctttgc caaccccttg caagggacag atgcccacgc aagccggccc aggcgaaacg
    19681 ttattccaat gccgcgcgcg agacggccgc cggtctcaat cctcgtctaa cagcctgtcc
    19741 gtctggcggc gctgcttctt ggtcgggcgc ccgctgccgg catcccgcag cggaaccatg
    19801 gcgccggcga cggcttcctt gcgaggcacg ggcggcggcg acatgtcttc gtagagcttt
    19861 ttcgcctcct ccgcaggacc acggcgcagg cctggcccaa tgattttcca aacggaaatc
    19921 cgccggtcga gcgtgagggt caacacgtcg cccggcttga ccagatcgga tgcctgcgct
    19981 gctttgtcgc gattgatgcg cacgcgcccc gccaccacca gcttggcggc cagcgagcgc
    20041 gatttcaccg cccgcgaaaa aaacagccac ttgtcgatgc gctggcgccc gtccgcgacc
    20101 atcggccttc agcattcggg cggcagcgcc ggacgggacg accgcatcgg cgttatttct
    20161 tcaattgctc gcgcagggcg gcaagcttgg cgaagggcga atccggatcg aaccgcaccg
    20221 gcctttcctc gcgcggcttc gactggaacg ccggcttgcc gtgtccctcg ccccggccct
    20281 cgttacgatt atccgggcga ttatcaggcc tgccaccttc acggtagggc ttgccgccct
    20341 gggccggcct gccgccgcca ccgggcttgc cataggcgcg gcctgcgccc tcgccctgcg
    20401 gcctgtcggc cgactggcca ccctcgcccg gcttgccccc ctcgcttggc ctacccttgt
    20461 agcggctgcg gtcaaagcgc ggacggccct tgccctggct cttttccgcg gcgtcaccct
    20521 gctcggcggc gggctggccg gtggccggca cgttattatt gcgcccctgc gcgccacgct
    20581 gccgattatc ctggcggtga ccgccgcgcg gccgctcgaa gcgggcctgc cgccacaaca
    20641 ggatcggctt tggcggctcc ccggcggctt ccaccttgcc gggcgtcgcg ccttcgtccg
    20701 tagcggcggc tgccgcatcg acggcctgcg tctcggccgc gccagcgacg gcatgagcga
    20761 cggcttggcc ggcatcaatc gaaggctcgg ctttctcgtc ggcatgtccg gcctcttcca
    20821 gcctcaggcc tgccgttgca tccgcctcta tcgcctcggc ttcctgcgcc agcagcgtcg
    20881 cttcggccac ttcggccggt gtctccggct gggtttccgc agcgacggtc attgctggcg
    20941 ccgtttcgac ggcaggttct accgccggcg tctcgcctgt gtcggagccg gccgacgccg
    21001 aaacgtcgcc cgacgcttcc tcctcgccgc cgcctgccgc ctcttcgacg ggaagcccgg
    21061 cggtggcgtc cgcctcgatt gcctcggcct cgaccgccgc aagcttggct tgcgccgcct
    21121 cgcgggcagc gttgtcctgc gcttcgagcc gcgccttcac ttccgtcgcc ggcttcggct
    21181 cggcacgata gccgagacct ttcagtattt cctccatgtc gtcggcattg gcgccgagaa
    21241 tggacatcat tgccggcgtc accatgaacg ccgtgccgtc atagccgcca tccgggcgcg
    21301 ggccgatacc cggcttccag ttggtcgcag ggcggatgag gtcggccagc cgttcgagaa
    21361 tgtcgatgcg cactgcgcgc cggcccagat tgcgatagcc ggcaagcttg tagaacgccg
    21421 gatcgaaggc tggatcgatg attaccgagg tgcggcccga agccagcgca tgaaccacgt
    21481 cgccaaaacc cggcttgtcc ttgccgtcgt tcttcagcgc ccacagcagg gtgatcagcc
    21541 ccgccggcgc cggcttgacc agcgacggaa cgaacacatg ataggcgccg aagcgcacgc
    21601 cgaggcggcg aaggccggcg cgcccttcct ggtcgagcgt cttcatttcc tcggcgatgt
    21661 cgcgccggtt gatgaggccg aaattctcga ccagctggaa ggcgatgccg cgcgcgatgc
    21721 cggaaatctg ctcggcgttc ttcagatcga ccagcggctt caacaaaagc tcgacctgga
    21781 agttgacgaa gcgatccgcc cgcgccgcca ccttgtcgcg cgccggtccg ttcaactgct
    21841 cgtcggccag cagcaccagg cgcggcttca gcgcgtcttc gccggcgacc agcgtgccga
    21901 tcggcgcgcc gatccagcgc agcgtgccgt ccgagccaag cgcgatgtcg ccattcgctg
    21961 ctgcgccgaa ccgctcggcg cgcgcctcga actcggcggc cagcgccttg tgcgcggcag
    22021 ccttcaccgc cttggcgtct tcgccgcccg cactttggtc ggcggtaaaa cggaacccct
    22081 gcaactcgcc gacatggtgg ccttcgacaa ggacggttcc ggcagggctt atttcggctt
    22141 caggcatcgt gttttctcta aggcgccgca tgaggacgga agtcctgcgg tctacaaaac
    22201 gtttcgtcaa ccgctcatgc agcgcatccg acaatctgtc ttcgatttcc cgcgtctttt
    22261 cctgccagtg tgcctgatcg gcaagccagc cgggccgatt ggaaacgaag gtccaggtgc
    22321 gtatctgcgc gatccggtgc gacagcgtgt cgatgtcgcc ctcggtggtg tcggcgcgcc
    22381 gcacctgttc ggccatgtag ttttcatcga catggccgcg tttcgccaga tccatataga
    22441 tggaggcaat caggtcggcg tgctgggccg gcgcaatcct tcggtagtcc ggcagcgcgc
    22501 aggcttccca caacagcgcc acccgctcgc ggcccgtcgc cagcgcgcga atgtcctcgt
    22561 cgcgcgaaag atgctccagc gcctgcgcgt ccaccgccgg cagcgcccgc gtcagcccct
    22621 cgaccggcgc attggcttcc attgaacgct tcagcccatc gaggcttgaa aagtcgaaac
    22681 ccgtggtgcg ccattgcagc acctttactg gctcgaaatc gtggccctcg atcttctgga
    22741 tcaaatcctc ttcgaacgga tcgacctggc cggttacgcc gaacgtgccg tcgcgcagat
    22801 gacgccccgc gcggccggcg atctggccaa gctcggcagc ggtcagctgg cggtattgaa
    22861 agccgtcgaa cttgcggttc tgggcaaagg cgacatgatc gacgtcgaga ttgaggccca
    22921 tgccgatggc gtcggtggcg atcaggtaat cgacatcgcc ggactggtag agcgccacct
    22981 gcgcgttgcg cgtgcgcggc gacagcgccc ccagcaccac cgccgccccg ccctgctggc
    23041 ggcggatgag ttcggcgatg gcatagacct catcggccga aaacgcgacg atggccgtgc
    23101 ggcgcggcaa tcgcgtcagt ttcttggagc cggcataggc cagatgagaa aggcgcggcc
    23161 gcgtgaccac ggaaatgccg cgcaacagct tctgcaagat gccctgcatg gtcgcggcgc
    23221 ccaaaagcag cgtttcctgg cgcccgcgca aatgcaggat gcggtcggtg aagatgtggc
    23281 cgcgctcaag atcgccggcc agttgcacct cgtccagcgc gacgaaggcg gcgtcggttt
    23341 cgcgcggcgt cgcctccacc gtgcagaccg aatatttcgc ccccttgggc tgaatctttt
    23401 cctcgccggt aatcagcgcg accttgttgg ccccgacctt ctcgcagaca cggccataga
    23461 cctcgcgggc aagcagccgc agcggcagac cgataatgcc gctttcatgc gccaccatcc
    23521 gctcgatggc gagatgcgtc ttgccggtgt tggtcggccc cagcaccgcg gtcacgtcgc
    23581 ggccggagag gatcagcggc tcggtctgtt tgggttggat gttcatcggc tcggaagaaa
    23641 gacctgcttg ccttgcattg ccgggagcgc gccgcaaaag cgcggatgat gaacgacaca
    23701 tagggatttg aacggggaag cgaaagcgga atgttttcga acgccgcgca gccgcttctg
    23761 gaacggcgcg atacgtggcc gattaacaag tggaacggga ctggaacgaa tcggcgacga
    23821 atcgctgact cacactgatt caggctttgt tcacggctaa atgtagattt tatatcgcta
    23881 aatttaccat atgctgaatc acatccgtca tccgcctcga cgagacgggt cgcgccgtta
    23941 accttagccg gcaagccatg ccgccatctt cgggtcgccc accgtaagcg cctgaaatct
    24001 ttctgatttt ttaacgatct tcaagcgccg cgcgcagccg gaccgttttc acgttaacgc
    24061 cttgtccaaa gccggaacga aacgacgacg aatcggcgat gcgcaacgtt tcccgtttcg
    24121 ttcaccccaa catcttgcgt tatgcacagc ttgtccaccg acgatccaca ggcggctgcg
    24181 cccgattccg acccgccgcg ccgccgatgt taacttttgc gtgccgcatt gagacaccag
    24241 caacggcggt cagcctcaac tctgcctgaa atcgccggca cggtcaggcg atatactggc
    24301 ccccattggc cgtcagtgtc gatccggtga tgaagccggc atcgtcggag gcaaggaata
    24361 ccacgcagcg cgcgatttcc tccggttcgc caagccgccc gaccggaatt tgcggaatga
    24421 tgcgctcgtt cagcactttc tcgtcgatcg ccttgaccat gtcggtgccg atatagccgg
    24481 gacagatgac gttgacggtg atgcccgcgc gcgccccctc ctgcgccagc gccttggtga
    24541 agcctatgtc gccagccttc gaggccgagt agttgacctg gcctgcctgc cccttctgcc
    24601 cgttgatgga ggagatggtg acgatgcggc cgaacttgcg ctcgcgcatg ccgttccaca
    24661 atggatgcgc catgttgaag gcgcccgaca gattggtgtc gatcacctct ttccattgct
    24721 gcggcgtcat tttgtggaac atggcgtcgc gggtgatgcc ggcattgttg accagcaccg
    24781 agaccgggcc gagatcggcc tcgacctgcg cgacgccggc aacgcaggca tcgtaatcgg
    24841 caaccgacca tctgtaggcc ttgatgccgg tttccgcgct gaacctctgc gccgcttctt
    24901 cattgccggc atagttcgcc gccaccgcat agccggcatt cttgaggccc acggaaatcg
    24961 cagcgccaat gccgcgcgta ccgccggtaa cgattgcagc cttcgtcatg cttgttctcc
    25021 cattatggaa agagcgaacc gggtgcggcg aatagcgaaa gggttagtgc ccgccgccga
    25081 cgccgttctt gccttgtccg ccttcctgtc ccctgatcgt caattcatgg cttcaacgca
    25141 catggccacg cccatgcccc caccgatgca caatgtggcg aggcccttgc gggcgccgcg
    25201 ccgcttcatt tcgaagacca gcgtattgaa gatgcgcgcg cccgaagccc cgatcggatg
    25261 gccgatggcg atggccccgc cattgacgtt gaccatcgcc gggtcccagc cgatgtcctt
    25321 gttgaccgcg caggcctgcg ccgcgaaagc ctcgttggcc tcgaccagat cgagatcgcc
    25381 gaccgcccag ccggcctttt ccagcgcacg gcgcgaggcc ggaatagggc cggtgcccat
    25441 gattgccgga tcgacgccgg ctgtagccca ggaagcgatg cgcgccaaag gtgtaatgcc
    25501 gcgccgcgcg gcttccgcct cgctcatcag cagcacgccc gccgcgccgt cattgatgcc
    25561 ggatgcgttg gcggcggtca ccgtgccatc cttgtcgaag gccggcttca gcttctgcat
    25621 ggcgtccatc gtcgcgccgt gacggatgta ttcgtcctgg tcgacgaccg tgtcgccctt
    25681 gcggcccttg accgtgaagg cgacgatctc gtccttgaac ttcccggcct tctgggcggc
    25741 ctccgccttg ttttgcgagg ccagcgcgaa cctgtcctgg tcgtcgcgcg tgatctggaa
    25801 ctgcttggcg acgttctcgg cggtggtgcc catatggtag ccgttgaagg catcccaaag
    25861 cccatccttg atcatggtgt cgatcagctt cagatcgccc atcttggtgc cggcgcgcag
    25921 atgctgcgca tgcgccgaca tcgacatgga ttcctggccg ccggcgataa tcacgtcggc
    25981 gtcgccgccc gctatctgct gcatgcccag cgcaatggcg cgcaggcccg aaccgcagac
    26041 ctggttaagc ccccacgccg tcgtttcctt gggcaggccg gcaatgatcg aggcctgccg
    26101 cgccgggttt tgcccctgcg ccgcggtcag cacctgtccg aggatgacct cgttgacttc
    26161 cgccgcatcg acgccggcgc ggacaagcaa ttcccggatc accgttgcgc caagttcatg
    26221 cgcgggcgtt gccgcaaacg ccccgttgaa cgatccgaca ggcgtgcgcg cggcgctggc
    26281 gacgacgatg gaattggcag cagacatttg attctccatt cggccatggc tcgcccgcca
    26341 gggcgatgaa ttagcggttg tttacttttc ttgccctttc caatcaccaa gtcaaaccgg
    26401 aaatggtcat gcccggcatg catgccggac aggccccgcc aagcggtgcg ccatatccca
    26461 ggcccaaaag cggtgccgcc gttcccgcac tgcacaaacc gcttggaatc ctgatgcctt
    26521 tgcgcatatc cttgaaaaat tgcgcaatct tgccggctac atgttttggg tacgccggtg
    26581 ccggggagga tgctgatggc agcaaaagac gaaccggtgg tgatcaagaa atacgccaac
    26641 cgacgcctct acaacacagg cacgagcacc tatgtgacgc ttgaggatct ggccgaaatg
    26701 gtcaagaagg gcgaggaatt cacggtgcag gacgccaaga ccggcgatga catcacccac
    26761 cccgtactga cgcagatcat cttcgagcta gagaacaagg atgggcagaa catgctgccg
    26821 gtgccgttcc tgcgccagct gatcgccttt tacggcgacc agatgcaggc catcgtgccg
    26881 agcttcctcg aacagtcgat gatggctttt tccaaggagc agtcgcggtt tcgcgagcag
    26941 atgaaggaat cgctggcgca atcgcccgta gaggtcatga aatcgccgat aaaggcgctt
    27001 gaggaacaga cccgccgcaa catggaaatg ttccagaacg ccatgcgcat gttcacgcct
    27061 tttccgcagg cgggagcagg cgctgccgcc gaacccgccg gcgccaccaa tcagcctagc
    27121 tccgatgcac cggaaaaacc gggcgacctg caagaactga aggaacagat cgcggcgatg
    27181 cagcgcaagc tcgacaacat gtcgtgaacg cgcttcttta ccggctgccc ggccgccata
    27241 tctatccggt cgtggcgagt aagcgcggga cgcctgaggg ccgtgcctgc cgtgccaccg
    27301 atcccgcaac gcgccggcct gacctgcaat gcgcctatcc tgatgtgtga cgacaaatga
    27361 cagaaccccg cctcgtcccg gtctttgacg gccacaacga cacgctgctc aagctctatc
    27421 tcgcttccga tcccgacaag gaaaagctgt tcatcgacgg ctcgcccgcc gcgtggcaca
    27481 ttgatctgcc ccgcgccaaa aaaggtggcc tcgtcggcgg catgttcgcc atttttccgc
    27541 cgcccgtcga cgagcgcaag cacgatgccg gccgccccgc gccgatgccc ggcaatgacg
    27601 agccgctgcc gccacaactg tcgatgaacg cggcgcgcgt ctcgacgctt ggcatggcgt
    27661 ctatcctgtt caggctggag cgcgccggcg cgctttccat ctgccgcagc gccgccgaca
    27721 ttcgcgcagc catggcgcgc ggttcgctcg ccgccgtctt ccatatcgaa ggcgtcgagg
    27781 ccatcgatcc cgaacttgcc atgctggacg tgctccacgc cgccggcctg cgctcgctcg
    27841 gcatcgtctg gagccgcccc aatgccttcg gcaacggcgt gccgtttcgc tttccctcct
    27901 cgcccgatac cggcgacggc ttgaccgacg ccggcaaggc gctggtcagg gcctgcaacc
    27961 ggctgaaaat ccagatcgat ctttcccacc tcaacgaaaa gggttttcgc gacgtggcgg
    28021 cattaagcga cgcgcctctg gtcgccacgc attcgaacgt ccattcgatc accgccagcg
    28081 cccgcaacct gaccgactgg caactcggcg ccatccgcga aagcaagggc atggtcggcc
    28141 tgaacttcgc caccggcttt ctcaatccgg ccggcccatc gggaagaggc gccgatatcg
    28201 ccatgatgat ccgtcatgtc gacgccatgc tggaagcgct gggcgaggat ggcgtcggac
    28261 tgggttccga tttcgacggc gcgcccgttc cttccgagat tggcgacgtt tccggcctgc
    28321 caaggctgat cgaggccttc gaaaagcacg gctacgggcc ggaactgatc gaaaagatcg
    28381 cctggaaaaa ctggatcggc gcgctcgaac gctcgatcgg gtaaattatc cggcaaatcc
    28441 catccgcttc agccaatcct tgccgacgcc gcggtcgcgg atgatcggca gcgggtcttc
    28501 acggtcgagc cgcgcgcaga tgcggtagac ctcgagcgcg ccgtcaccga tctcgccgcg
    28561 cttgtgaaaa aacatcgccg ccgcataacg ggtgcggccc gaccattcct cgccaagcgg
    28621 cgtgttgacc agcgcccatt gctcggctgc ctcgtcatcc gcttccccgg ccatggttca
    28681 aaaatccgcc ggcgggttgg cggtgcggcg gtcgagcttg atgaaaggcc tttgcaccac
    28741 cttcgcgcgc ttcatcatct tctggtactg caactcgcgc atcgcgtaga tctcgaccca
    28801 cagatcatcg acc
//
LOCUS       DASYCS010000456         7418 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3184212,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000456 DASYCS010000000
VERSION     DASYCS010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 7418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7418
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3184212"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="VGM46_14140"
     CDS             complement(<1..226)
                     /locus_tag="VGM46_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010661610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP endonuclease"
                     /protein_id="HEY4193782.1"
                     /translation="MRRPQMIFQPLILLAMLAIAAALVFGFFGALHPALDSFSHLRAH
                     LAVLLALLALALLAFAGFRTEAAVALLFAFG"
     gene            316..912
                     /locus_tag="VGM46_14145"
     CDS             316..912
                     /locus_tag="VGM46_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007529883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF922 domain-containing protein"
                     /protein_id="HEY4193783.1"
                     /translation="MKFEQWPYVLALAASVCVAQARAQGQPVEQEQSYAITGASGPEL
                     YASIGEHGPLVGGGVRAIAHTNFKLTWTRNYVPQGSACVLVSARPKLVITYTLPQPAN
                     LSAALQRRWNVFIDGIRRHERVHGDHIKAMVKRIEATTIGVRVENDPSCRKIREQLKG
                     PLSQASLAQRQQSRDFDRVEMGEGGTIRLLILALVNGG"
     gene            complement(1027..2526)
                     /locus_tag="VGM46_14150"
     CDS             complement(1027..2526)
                     /locus_tag="VGM46_14150"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HEY4193784.1"
                     /translation="MENRIYPQAIGSVLMPEPVAPQSFSDAEEAVVALEKLYVRNTSF
                     LRDSFSALAAGGDNSRRYRAFYPQVSVTTTSFGHVDSRQAYGHMPTPGTFSTTITRPD
                     LFHSYLVEQLRIIMRNHGVPVTVSQSSTPIPLHFAFLEGTHVDGSTAERIKRPIRDLF
                     DVPDLDGTDDQIANGTFEVVLGEARPLAPFTAQRVDYSLHRLSHYTATSPQHFQNFVL
                     FTNYQVYIDEFAARARELMAKGGEGYSEFVEPGNIITRARERKSDDAAAVRLPQMPAY
                     HLKKPNHGGITMVNIGVGPSNAKTITDHVAVLRPHAWLMLGHCAGLRNTQELGDYVLA
                     HAYVREDHVLDDDLPVWVPIPPLAEIQVALQEAVQEVTGLSGYDLKRIMRTGTVATID
                     NRNWELRDQRGPVQRLSQSRAIALDMESATIAANGFRFRVPYGTLLCVSDKPLHGELK
                     LPGMATEFYKRQVAQHLTIGIRAMEKLSEMPMERLHSRKLRSFSETAFQ"
     gene            complement(2603..2797)
                     /locus_tag="VGM46_14155"
     CDS             complement(2603..2797)
                     /locus_tag="VGM46_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193785.1"
                     /translation="MTSFAARAAQTLPILLRATLILLALAAPVLAVPTFRAVNQTIEA
                     PTMRKGSGVGFVLLVSLQRG"
     gene            complement(2817..2972)
                     /locus_tag="VGM46_14160"
     CDS             complement(2817..2972)
                     /locus_tag="VGM46_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006199743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193786.1"
                     /translation="MLARRFTIVCLLTTLFGMGFAILVAQAERQPRAWDDAGNCRTIV
                     CQSHINY"
     gene            3145..3867
                     /locus_tag="VGM46_14165"
     CDS             3145..3867
                     /locus_tag="VGM46_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009452220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HEY4193787.1"
                     /translation="MTTQSAAIELYYWPTPNGHKISIMLEELGVPYEVKYINIGKGEQ
                     FAPSFLKIAPNNRMPAIIDPEGPSLDGKAGAAPISVFESGAILQYLGRKFGKFYSTDE
                     RQRTEIDQWLFWQMGGLGPMAGQAHHFRQYAPEKIPYAVDRYTNEVNRLYGVLDKRLA
                     DREYLAGDYSIADMASFPWTRSHKNQGQDLEDFPNMKRWFEAIAARPAVQRGVEIGQE
                     HRRNLADDQEAQKVLFGQKARG"
     gene            complement(3937..4278)
                     /locus_tag="VGM46_14170"
     CDS             complement(3937..4278)
                     /locus_tag="VGM46_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006199744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HEY4193788.1"
                     /translation="MFAKAGLAVAAMLVLTSAALADPIEGNWKTKDGDTAAISSCGGS
                     FCIKLTTGKYAGKSIGTMSAGGANSYSGSITKPSNGKTYSGSAKLSGSSLKLSGCVLA
                     VLCESQTWNKL"
     gene            4727..4993
                     /locus_tag="VGM46_14175"
     CDS             4727..4993
                     /locus_tag="VGM46_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018427001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEY4193789.1"
                     /translation="MARFEMQEAGFGAMGATAQADILFELGMMYATGRDCETDVVAAH
                     KWFNIAAIKGSLRAASLRSELSATMSKIEIARALREAREWMTMH"
     gene            5331..6452
                     /locus_tag="VGM46_14180"
     CDS             5331..6452
                     /locus_tag="VGM46_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY4193790.1"
                     /translation="MKKTKLLGAALFGLAMGVSAAYADDINIAVAGPMTGQLATIGDQ
                     FKQGAQAAAAAINAAGGVDGRQIKLSMEDDQCDPKQAVSVANRIVANGVKFIDGHACS
                     GSSIPASAVYAEAGALMMSPASSNPVMTDDAAAKGWNTIMRLYTRDDAQGAFIGPWIA
                     KKYAGKNLVILHDKSAYGQGIADAVKATANEGGLKEVMYEGINAGEKDYSALVTKLKD
                     LKADVVYFGGYHPEAGLIMRQAAEQNLKFQLIMPDSIASPEFWQVAGPAGEGTMFVFP
                     SDPQAKPEAKEAIEKIKAGGFVPEGFTLFSYATVQAIAEGIKRAGSDDPAKVAEALKD
                     GKPIDTVVGPVTFDKKGDLMNASYDINQWHDGKYAPIPQ"
     gene            complement(6519..>7418)
                     /locus_tag="VGM46_14185"
     CDS             complement(6519..>7418)
                     /locus_tag="VGM46_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010915076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEY4193791.1"
                     /translation="GTLHHLSFPPAPPSGSQMRIETGVTSGDAVTPFYDPMIAKIVVH
                     ADDRAAALGALAKAIEGTEIAGSVTNLAFLAALARDADFAAGDVDTGLIGRKQDELTR
                     PAEVSPETVARAVLVASGLDRPGPSPDPWNQLQGYAHFFGTPRRTLLKKGETSILARV
                     APAGNNRFSVTVDGDRERLFNAPTGLDFGARFARWPDHVTIFENGHSFNFEVPDPLAK
                     GAETAAGTASLRAPMPGLVKLVRAAKGDAVLKGQPLLILEAMKMEHTIAAPHNGVIAE
                     IAAEGAQVTDGTVLVKFAEDTQV"
BASE COUNT         1495 a         2235 c         2334 g         1354 t
ORIGIN      
        1 cgccgaaggc aaacagcagc gctaccgccg cctcggtgcg aaaccccgcg aacgccagca
       61 gcgccagcgc cagaagcgcc agcaggacgg caaggtgagc gcgcaaatgc gagaaggaat
      121 cgagcgccgg atgcagcgcg ccgaagaagc cgaacaccag agcggcggca attgccagca
      181 tggcaagcag gatcagcggt tgaaatatca tctgtggccg gcgcatggga tagtccgaaa
      241 caatgtcgtt catcgacggt aaaatgtgtc acgcatatct tttgcgcgaa attgcggcgg
      301 cgcggtgagg gaccgatgaa attcgaacaa tggccttatg tccttgcttt ggcggcaagc
      361 gtttgcgtgg cgcaggcgcg cgcgcaaggg cagcctgtcg agcaggagca gagttatgcc
      421 attacaggcg cttccggccc ggagctgtat gcctccatcg gagagcatgg accgctggtc
      481 ggcggtggcg tccgcgccat cgcgcacacc aatttcaagc tgacctggac cagaaactac
      541 gtgccgcagg gcagcgcctg cgtgctggtt tcggcgcgtc cgaagcttgt catcacctat
      601 acgttgccgc agccggcaaa cttgtcggca gcacttcaaa ggcgctggaa tgtcttcatc
      661 gacggcatcc gccgccatga gcgcgtgcat ggcgatcaca tcaaggccat ggtgaagcgc
      721 atcgaagcca ccacgatagg cgtgcgcgtc gaaaacgacc caagctgccg caaaatcagg
      781 gaacagctca aggggccgct gtcacaggca tcccttgcgc agcggcaaca aagccgcgat
      841 ttcgaccgcg tcgagatggg cgagggcggc accatccgac tgctgatcct tgcgctggtg
      901 aacggcggct agagcaaggc ggaacgagcc taccgatctt gccgcggctt ttttttacgc
      961 ccgcagcttt taatgcatgc cgtcatcgca aaaaccgccg cacagttttg cgcgacagga
     1021 acggcgctat tgaaacgccg tctccgagaa actccggagc tttctggaat gcagccgctc
     1081 catcggcatt tcggaaagct tttccatcgc ccttatgccg atggtcagat gttgcgccac
     1141 ctggcgcttg tagaattcgg tcgccatgcc cggcagcttg agttcgccgt gcagtggctt
     1201 gtcggaaacg caaagcaatg tgccgtaagg cacgcggaag cggaagccgt tggccgctat
     1261 cgtggccgat tccatgtcga gcgcgatcgc gcgcgactgc gacaggcgct gaaccggccc
     1321 gcgctggtcg cgcagttccc agttgcgatt gtcgatggtg gccacggtgc cggtgcgcat
     1381 gatgcgcttc aggtcgtagc cggaaaggcc ggtcacctcc tgcaccgcct cctgcaacgc
     1441 cacctgtatt tcggccagcg gcgggatagg cacccacacc ggcagatcgt cgtccagcac
     1501 atggtcctcg cgcacatagg catgcgccag cacatagtcg cccagttcct gcgtgttgcg
     1561 caggccggca cagtggccga gcatcagcca cgcatgcggg cgaagcaccg cgacatggtc
     1621 ggtgatggtc ttggcgttgg acgggccgac gccgatattg accatggtga tgccgccatg
     1681 gttcggcttc ttcaaatgat aggccggcat ttgcggcaag cgcaccgccg cggcgtcatc
     1741 cgattttcgc tcgcgcgccc gcgttatgat gtttcccggt tccacgaatt cagaatagcc
     1801 ttcgccgccc ttagccatca gttcgcgcgc ccgcgccgcg aactcgtcga tatacacctg
     1861 gtaattggtg aacagcacga agttctggaa atgctgcggg ctggtcgccg tatagtggga
     1921 aagccggtgc agcgaataat cgacgcgctg cgcggtgaat ggcgccagcg gtctcgcctc
     1981 gcccagcacc acctcgaacg tgccgttggc gatctggtcg tcggtgccgt caaggtcggg
     2041 tacgtcgaac aggtcgcgga tcggccgctt gatgcgttcg gcggtcgagc cgtcgacatg
     2101 ggtgccttcc aggaaggcaa agtgcaaggg aatgggcgtg ctcgattgcg acaccgtcac
     2161 cggtacgccg tggttgcgca tgatgatccg aagctgctcc accaggtagg aatggaaaag
     2221 gtcgggccgc gttatcgtgg tggaaaaggt tcccggcgtc ggcatgtggc cgtaggcctg
     2281 ccgggaatcg acatggccga aggaagtcgt ggtaacgctg acctgcgggt agaacgcgcg
     2341 gtatcgccgg ctgttgtcgc cgccggccgc cagtgccgaa aatgaatcgc gaaggaagga
     2401 agtgttgcgc acatatagtt tttcaagcgc gacaaccgcc tcttcagcat cgctgaagct
     2461 ttgcggggcg acgggttcgg gcatgagcac ggagccgatg gcctggggat agattcgatt
     2521 ttccatggct cattatagag atttgcgccg ggctggggag gggagccgtc gctgaacccg
     2581 gccaaagagg cgtctttatt tttcaaccgc gctgcaagct gaccagcagc acgaaaccga
     2641 cgccgctgcc cttgcgcatc gtgggagcct cgatcgtctg gttgacggcc cggaatgtcg
     2701 gcaccgccag caccggagcg gcgagggcca gcaggatcag ggtcgcccgc agcaggatgg
     2761 gcagggtttg cgcggctctg gcggcgaagc tggtcatggc gtttgccgcc ctgtggtcag
     2821 tagttgatgt gggactggca gacgatcgtc cggcaattgc cggcgtcgtc ccaggcgcgg
     2881 ggctggcgct cggcctgcgc gaccagaatg gcgaagccca tgccgaacag cgttgtcagc
     2941 aggcagacga tggtgaagcg gcgggcaagc atgggtggcg tctcctctta tgcgcaagag
     3001 attgcaccct gccggctgaa ccggctgtga tgcggccatt catctgccgt tcaaatttat
     3061 tgacaatgct ttgtatcgat ggccgcttgc gttcgcgccc tgccggctta tctccatcgc
     3121 agaccaatag cccggagatc aggcatgacg acgcagagcg cagcaatcga gctttattac
     3181 tggccgacgc caaacggcca caagatttcg atcatgcttg aggaattggg cgttccctat
     3241 gaggtcaaat acatcaatat cggcaagggc gaacaattcg cgccatcgtt cctgaagatc
     3301 gcgccgaaca accgcatgcc ggccatcatc gatccggaag ggccgtcatt ggacggaaaa
     3361 gcgggcgccg caccgatttc cgtattcgaa tccggcgcta ttcttcaata tctgggccgg
     3421 aaattcggca aattctattc gacggatgag cgccagcgca ccgaaatcga ccaatggctg
     3481 ttctggcaga tgggcgggct tgggccaatg gctggccagg cgcaccattt tcgccagtat
     3541 gcgccggaaa aaatccccta tgccgtcgat cgctacacca atgaggtcaa ccgcctttac
     3601 ggcgtgctgg acaagcggct ggccgaccgt gaatatctgg ccggcgatta ttccatcgct
     3661 gacatggcgt ccttcccctg gacgcgctcc cacaagaacc agggccagga tctcgaagat
     3721 ttccccaaca tgaagcgttg gttcgaggcg attgccgccc gcccggcggt tcagcgcggg
     3781 gtcgagatcg gccaggagca tcgccgcaac ctggcggacg accaggaagc ccagaaggtg
     3841 cttttcggcc agaaggccag gggttgagga agagatagcc ggcggaaaac gaaaatcggg
     3901 gaacgatgat cgttccccga accgccggtc aggcgctcaa agcttgttcc aggtctggct
     3961 ttcgcacaac acggcgagca cgcagcccga cagcttcagc gaactgcccg aaagcttggc
     4021 gctgcccgaa taggttttcc cgttggaggg cttggtgatc gagccgctat agctgttggc
     4081 gccgccggcg ctcatcgtgc cgatggattt tcctgcatat ttgccggtcg taagctttat
     4141 gcagaacgat ccgccgcaag aactgatggc ggcggtgtcg ccgtccttcg tcttccagtt
     4201 tccctcgatg gggtcggcaa gcgccgcact tgtcaggacc aacatggccg caacggccag
     4261 cccggcttta gcaaacatcg caatcctcca tttggcgccc ggccggctgc ttccgccggc
     4321 ctctatcgtt tacgcaaacg taagctaatg cagccgccct taaaacaaaa gcggtgccgc
     4381 aacggaagaa aagagccttt ttgaattggc gaatggccgc gcttcggggc gtttataaac
     4441 accatgccag cgcatcgatg tttcccgtgg ttagcgaagt cttgacccgg acgcggtgga
     4501 ttttcatcga atttgaaagg attccgcaca gaagcgggat ttgtatcctt aacgatttct
     4561 cgcgtttact tcttgtttta gctttgtttt ctccaaatta agcacgccat ttaacgacgg
     4621 attcaagcct gcgcggcatg cttcgaagca tcggaagagc aagtctccca gccagaattc
     4681 aggggacgaa ggaggcggct ctcaggatcg agacagggga ttgaaaatgg cacgttttga
     4741 aatgcaggaa gctggtttcg gcgcgatggg cgcaaccgcc caggccgaca ttctcttcga
     4801 attgggcatg atgtatgcga ccggccgcga ttgcgagacc gatgtcgtcg ccgcccataa
     4861 atggttcaac atcgccgcga tcaagggttc gctgcgggcg gcaagcctgc gctcggaact
     4921 ttccgcgacc atgtcgaaaa tcgaaatcgc ccgcgcgctc cgtgaagccc gcgaatggat
     4981 gacgatgcac tgaccatagg acgtagcgac agggcaaggc accggatcag cgccgcacca
     5041 agacggcgcg accggcaaga ccaagatgcg gcgccgcacg agggcagccg tgcatgacag
     5101 ggaagcggga gatcagtggt gcgggggcac cgggggatcg ctcggcaggc aagccgctac
     5161 cggccgggac aaggccggag cggcttttgc catttatccg gtattttcaa gaacaaacgg
     5221 gcgttagctg actcaatgct ccctctggac agaagcgcag aagaatggaa ttgtcgccgc
     5281 cattctgagt aagcgctgcc tggtggccgc gcatagtggg gagggttcag atgaaaaaaa
     5341 caaaattgct cggcgcggca ttgttcggct tggccatggg cgtgtcggcg gcctacgccg
     5401 atgatatcaa catcgccgtt gccggtccga tgaccggcca gcttgccacc atcggcgacc
     5461 agttcaagca gggcgcacag gccgccgccg ccgccatcaa cgcagccggc ggtgtcgacg
     5521 gacgccagat caagctttcg atggaagacg accagtgcga tcccaagcag gcggtttccg
     5581 tcgccaaccg catcgttgcc aacggcgtca agttcatcga cggccatgcg tgctcgggtt
     5641 ccagcattcc ggcgtccgcc gtttatgccg aggccggcgc gctgatgatg agcccggctt
     5701 cctccaaccc ggtgatgacc gacgacgcgg ccgccaaggg ctggaacacc atcatgcgcc
     5761 tctacacgcg cgacgatgcg cagggcgcct tcatcggccc gtggatcgcc aagaaatatg
     5821 ccggcaagaa cctcgtcatc ctgcatgaca agagcgccta cggccagggc attgccgacg
     5881 ccgtcaaggc gaccgccaac gaaggcggcc tcaaggaagt catgtatgaa ggcatcaatg
     5941 ccggcgagaa ggactattcc gccctcgtca ccaagctgaa ggacctcaag gccgacgtcg
     6001 tctatttcgg cggctatcat ccggaggcgg gcctgatcat gcgccaggca gccgagcaga
     6061 acctcaagtt ccagctcatc atgccggatt cgatcgcttc gccggaattc tggcaggttg
     6121 ccggcccggc cggcgaaggc acgatgttcg tgttcccgtc cgatccgcag gccaagccgg
     6181 aagccaagga agccatcgag aagatcaagg ccggcggttt cgtgccggaa ggcttcacgc
     6241 tgttctccta cgccaccgtc caggcgatcg ccgaaggcat caagcgcgcc ggcagcgacg
     6301 acccggccaa ggtcgccgag gccctcaagg acggcaagcc gatcgacacc gtcgtcggcc
     6361 cggtcacctt cgacaagaag ggcgacctga tgaacgccag ctacgacatc aaccagtggc
     6421 acgacggcaa atacgcgccg atcccccagt aaaaagcctc gcaggcaata gaaaaaatgg
     6481 ccgggttttc ccggccattt ttatttgcgc ccggcctgtc agacctgagt atcttccgcg
     6541 aacttgacca gcacggttcc gtcggtgacc tgcgcgcctt ctgccgcgat ttccgcgatg
     6601 accccgttat gcggcgcagc gattgtgtgc tccatcttca tcgcctccag gatcagcagc
     6661 ggctgtcctt tcaggaccgc atcgcccttg gcggcgcgca ccaactttac cagtccgggc
     6721 atcggcgcgc gaaggctggc cgtgccggcg gcggtttccg cacctttggc aagcgggtcg
     6781 ggaacctcga aattgaagct gtggccattc tcgaagatcg tcacatggtc cggccagcgc
     6841 gcgaagcgcg cgccgaaatc gaggccggtg ggcgcgttga acaggcgctc cctgtcgcca
     6901 tccaccgtca ccgaaaagcg gttgttaccg gcaggggcca cgcgcgcaag gattgatgtc
     6961 tcgcctttct tcagcagggt gcgccggggc gtcccgaaaa aatgcgcgta gccctgtagc
     7021 tggttccatg gatcgggcga ggggcctggc cggtcgaggc ccgaagcgac gagcacggcg
     7081 cgggcaacgg tttcgggcga cacttcggcc ggccgcgtca gctcgtcctg cttgcggccg
     7141 atcagcccgg tatcgacatc gccggcggca aagtcggcat cgcgcgccag cgcggcgagg
     7201 aaggcgaggt tcgttaccga tccggcgatc tcggtgccct cgatggcctt cgcgagcgcg
     7261 ccaagtgccg ccgcacgatc atcggcatgg acaacgatct tggcaatcat cgggtcgtag
     7321 aagggcgtga cggcgtcgcc gctggtcaca ccggtttcga tgcgcatctg cgaaccgctt
     7381 ggcggcgcgg gcgggaacga caggtgatgc aacgttcc
//
LOCUS       DASYCS010000457         5116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_282967,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000457 DASYCS010000000
VERSION     DASYCS010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 5116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5116
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282967"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="VGM46_14190"
     CDS             <1..437
                     /locus_tag="VGM46_14190"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018239961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY4193792.1"
                     /translation="FDRARPIIEAYARMIGLMGPAGSGQLTKMINQIAIAGLVQGLAE
                     GIHFGKKAGLDIEKVVEVISKGAAGSWQMENRHKTMNAGKYDYGFAVDWMRKDLGICL
                     EEADRNGASLPVTALVDQFYKEVQAMGGKRWDTSALLARLER"
     gene            complement(477..968)
                     /locus_tag="VGM46_14195"
     CDS             complement(477..968)
                     /locus_tag="VGM46_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015317849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY4193793.1"
                     /translation="MDRLDKKILRLLQEDATLAVADVAKKVGLSTTPCWRRIQKLEEE
                     GVIKRRVALLDAGRINVRVTVFVSIRTNSHSHEWLRRFSEVIQEFPEVIEFYRMSGDV
                     DYLLRVVVPDIAAYDAFYKRLISKIEIRDVSSSFAMEQIKYTTELPLEYMVLDKESGA
                     NAA"
     gene            complement(1074..1691)
                     /locus_tag="VGM46_14200"
     CDS             complement(1074..1691)
                     /locus_tag="VGM46_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEY4193794.1"
                     /translation="MSETLESLTARIRACRICVEEPIGQPLPHEPRPVLVTSTKARIL
                     IASQAPGTKVHLSGKPFTDASGDRLRDWLGVGDGEFYDPDKFAIVPMGFCFPGQDAKG
                     GDLPPRRECAVQWRAPLLSLMPQIDLVLTIGIYAQAWHMGNARHASLSETVMNWRAAW
                     DASPARKVLPLPHPSWRNSGWLKKNPWFETDLLPFLKTEIRCRID"
     gene            complement(1693..2304)
                     /locus_tag="VGM46_14205"
     CDS             complement(1693..2304)
                     /locus_tag="VGM46_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008875843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEY4193795.1"
                     /translation="MKLFDGGKAPNPRRVRIFLAEKGIEVPLIPVDMGALEHRQDLVS
                     SRNRLKRLPVLELDDGTIITESVAICRYFEELHPDPALLGKGAVGKALVEMWQRRMEL
                     NLMNFVAAAFRHIHPAMKEWEIPQVPEWGEANKPKAVEFLKFLDDELAGREFIAGDAY
                     CIADITGMVAIDFMKPARIQMPQECGNVLRWYKSLASRPSASA"
     gene            complement(2347..2955)
                     /locus_tag="VGM46_14210"
     CDS             complement(2347..2955)
                     /locus_tag="VGM46_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEY4193796.1"
                     /translation="MSLLPRRASRPQAARWWRKLADYGLTALLLGLLILIAARLDRSN
                     MQDHQGRAVINDGDSITLGTVRVRLLGIDAPEYLQLCHGQSGDYACGKRAREALVKLI
                     GNRPVSCKGARHDRYGRLLGDCTAGHTRLNQAQVEAGWAVAYGDFEAEEASARAARLG
                     IWAGTFERPQDWRRDKGVAPEPAHDDILARAGDWLRQILRFW"
     gene            3029..4552
                     /locus_tag="VGM46_14215"
     CDS             3029..4552
                     /locus_tag="VGM46_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019858130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY4193797.1"
                     /translation="MSLQPGSTADKNIVDRRKPHRNSDVSRAVRRTRDRLSQRAGSHD
                     FDRELLKLHARAMTGSAVAIPLLVVTIAAAGLAVGMSGTILIWALLTVSCNAALAFVA
                     RYVDRAEAADLKPRQTRNGFLAAHFLSGLGWAYFASLSCDACAVAQFPVLKAVLLLLA
                     MAATAIVASSLRGALLVTFAVPVAVYAYFAARLWLPVEGIMAGLLFVALPFFDFVASR
                     LNRSTVMLLSFRTEKDGLIAELETAKSMSDEARRRAEEANLAKSRFLASMSHELRTPL
                     NAILGFSEVMADEVLGPMNNATYRDYARDVHDSGQHLLNLINEILDLSRIEAGRYQLN
                     EEPVVLPNIAEDCCHMMELRARNKDIRIVQDFERALPRLFGDERAVRQIILNLLSNAI
                     KFTATGGEIRVRVGWTSGGGQYVAVKDNGPGIPEDEIPVVLSAFGQGSIAIKSAEQGT
                     GLGLPIVQGLVAMHGGEFELHSKLREGTEAIAIFPLSRVMEELPALPMKAVAGGRRR"
     gene            complement(4628..>5116)
                     /locus_tag="VGM46_14220"
     CDS             complement(4628..>5116)
                     /locus_tag="VGM46_14220"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006333687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HEY4193798.1"
                     /translation="ALLAAILGGKGSDWVGKGSGADADMMVRKADIVDAALAFHGNSL
                     KDPLEALRRVGGREFAAIAGAILAARMQKIPVLIDGFAALAASAVLHAVNPDAISHCL
                     LASLPALPALAAAGEKLGLDAPLFDLALDHGEGVGAALGAGIVKAAALCSSGMAAAVR
                     EH"
BASE COUNT         1013 a         1646 c         1476 g          981 t
ORIGIN      
        1 ccttcgaccg cgcgaggccg ataatcgaag cctacgcgcg aatgatcggg ttgatggggc
       61 cggcaggttc gggacaactc accaagatga tcaaccagat cgccattgcc ggcttggttc
      121 agggccttgc agaaggcatc cacttcggca agaaggccgg gctcgacatc gagaaagtag
      181 tcgaggttat ctctaaaggc gccgccggat cctggcagat ggagaaccgc cacaagacca
      241 tgaatgccgg caaatatgat tatggatttg ccgtcgactg gatgcgcaag gatctcggca
      301 tctgccttga agaagccgac cgcaatggcg ctagcctgcc ggttacggcg ctggtcgacc
      361 agttttacaa ggaggtgcag gccatgggcg gcaagcgctg ggatacgtcg gcgctgctgg
      421 cgcggctgga aagatagccg ccgctagccc tgccggacag aaagccggcg cgccgatcag
      481 gcggcgttcg cgcccgattc cttgtccaga accatatatt ccagcggcaa ttcggtcgtg
      541 tacttgatct gctccatggc gaaggacgac gacacgtcgc ggatttcgat cttggaaatc
      601 agccgcttgt agaaggcatc ataggccgcg atgtcgggaa ccaccacacg cagcagataa
      661 tccacgtcgc cgctcatgcg gtagaattcg atgacctcgg gaaactcctg aatgacctcg
      721 gagaaccgcc gcagccactc atggctgtgt gaattggtgc ggatcgagac gaacacggtg
      781 acgcgcacat tgatcctgcc ggcgtcgagc agcgccaccc ggcgcttgat gacgccttct
      841 tcctcaagct tctggatacg ccgccagcag ggcgtggtcg acagcccgac cttcttggcc
      901 acgtctgcca cggccagcgt tgcatcttcc tgcaaaaggc gcaggatttt tttgtccagg
      961 cgatccattg ggcgctcccg agaaaaggtt gctcataatc tccggtattg cagcccataa
     1021 caagaaagaa attctgctcc acagcgcaaa accagggcgt ttattgcgtc tggttagtca
     1081 atccggcaac gtatttccgt tttcagaaac ggcaacaaat ccgtttcgaa ccatggattc
     1141 tttttcagcc agccgctgtt gcgccacgac ggatgcggca gcggcagaac cttgcgtgcc
     1201 ggggaagcat cccatgccgc acgccagttc atcaccgttt cgcttagcga ggcatgcctg
     1261 gcgttgccca tatgccatgc ctgcgcataa attccgatgg tcagcacgag gtctatctgc
     1321 ggcatcagcg acagcagcgg ggcgcgccat tgaacggcgc attcgcggcg cggcggcagg
     1381 tcgccacctt tggcatcctg ccccggaaag caaaagccca tcggcacgat ggcgaacttg
     1441 tccggatcgt agaactcgcc gtcaccgacg cccagccagt cccgcaacct gtcgcccgat
     1501 gcgtcggtga acggcttgcc gctcaaatgc accttggtgc ctggcgcctg gctggcgatc
     1561 aatatgcgcg ccttggtcga tgtcaccaag acagggcgcg gctcatgcgg caagggctgg
     1621 ccgatcggct cttccacgca gatgcggcaa gcgcgaatgc gcgccgtcag gctttcaagc
     1681 gtctcgctca tctcaggccg aggcacttgg ccggctggcc aggctcttat accaacgtaa
     1741 tacgttgccg cactcctgcg gcatctggat gcgcgccggc ttcatgaagt cgatggccac
     1801 catgccggtg atatcggcga tgcaataggc atcgccggcg atgaattccc tgcctgccaa
     1861 ttcgtcatcc agaaatttca ggaactcgac cgccttcggt ttatttgcct cgccccattc
     1921 gggaacttgc ggaatttccc actctttcat cgccgggtga atgtgacgga aggccgccgc
     1981 cacaaaattc atgaggttca gttccatgcg ccgttgccac atttcaacca gcgccttgcc
     2041 taccgcgcct ttgccaagca gggccgggtc gggatgaagc tcctcgaaat accggcagat
     2101 cgccacggat tcggtgatga tcgtaccgtc gtccagttcc agaaccggca atcgtttcag
     2161 cctgttgcgt gaactcacca gatcctgcct gtgctccagc gcgcccatat cgaccggtat
     2221 aagcggcact tcaatgcctt tctcggcgag gaatatgcgc acacgccgtg ggttgggggc
     2281 cttgccacca tcgaagagct tcatgcgttt ctcctgcccg aattcctcac gactataaag
     2341 cgcccactac caaaaccgaa ggatttgcct cagccagtcc cctgcccgtg caagaatatc
     2401 gtcatgcgcc ggttcgggag ccacgccctt gtcgcgccgc caatcttgcg gacgctcgaa
     2461 cgtcccggcc cagatgccaa gcctggcggc gcgcgccgag gcttcctcgg cctcgaaatc
     2521 gccatatgcc acggcccagc ccgcctcgac ctgggcttgg ttgagcctgg tatgtccggc
     2581 ggtgcaatcg cccagcaacc ggccataccg gtcgtgccgc gcgcctttgc aggaaaccgg
     2641 ccgattgccg ataagcttca ccaacgcctc acgggcgcgc ttgccgcagg catagtcgcc
     2701 gctttggcca tggcaaagct gtaaatattc cggcgcatcg atgccgagca gccttacccg
     2761 aacggtgccg agcgtgatgg aatcgccatc gtttatcacc gcccgcccct ggtgatcctg
     2821 catgttggaa cgatccaacc gcgccgcgat cagaatcagc aacccaagca gcagcgccgt
     2881 cagcccataa tcggcgagct tgcgccacca cctggccgcc tgcggccggg aagctcggcg
     2941 cggcaataaa ctcatatgga aaacagtctc ttaatcattg caatgtactg tcgaaagctg
     3001 aaccactgcc cgtaaaattg aagtatccat gtcgctgcaa cccggaagta cggcggacaa
     3061 gaatattgtg gaccgccgca aaccccatcg caacagcgat gtatcgcgcg ctgtgcgaag
     3121 gacgcgcgac cgtctttcgc agcgcgccgg cagccatgat ttcgaccgcg aactcctaaa
     3181 actgcacgcc cgcgccatga ccggcagcgc ggtggccatt ccgcttctgg tggtgacgat
     3241 tgcggcggcg ggactggccg ttggaatgag cggcacgata ctcatctggg cgctgctcac
     3301 cgtttcctgc aacgcggccc tcgccttcgt cgccagatat gtcgaccgtg ccgaagctgc
     3361 cgacctcaaa cctcgccaaa cccgcaacgg cttcctggcc gcgcatttcc ttagcggcct
     3421 cggctgggcc tacttcgcct cgctcagttg cgatgcctgc gccgtcgccc agtttccggt
     3481 actcaaagcc gtgctgttgc tgcttgctat ggccgccacg gcaatcgtcg cctcgtcgct
     3541 gcgtggcgcg cttctggtca cctttgcggt tccggtggcg gtctatgcct atttcgccgc
     3601 ccggctttgg ctgcccgtcg aaggcatcat ggcggggctg ctgttcgtcg cgctgccgtt
     3661 cttcgacttt gtggcaagcc gcctcaaccg ctcgacggtc atgctccttt ccttccgcac
     3721 ggaaaaggac gggctgatcg ctgagctgga aacggcgaaa tcgatgtcgg atgaggcgag
     3781 gcgacgggcc gaggaagcca atctcgcaaa atcgcgcttc cttgcctcaa tgagccatga
     3841 gttgcgaacg cccctcaacg ccatcctcgg cttttccgaa gtgatggccg acgaggtatt
     3901 ggggccgatg aacaacgcca cctaccgcga ttatgctcgc gatgtgcacg attccggcca
     3961 gcatctgctc aacctcatca acgaaattct cgacctgtcg cgtatcgagg ctggccgcta
     4021 ccaattgaac gaagagccag tggtgctgcc caatatcgcc gaagattgct gccatatgat
     4081 ggagttgcgg gcgcgcaaca aggacatccg catcgtccag gatttcgaac gggcgctgcc
     4141 gcgcctgttt ggcgacgagc gcgccgtgcg gcagatcatc ttgaacctgc tttccaacgc
     4201 cataaaattc accgccaccg gcggcgagat ccgtgtccgc gtcggctgga cttccggcgg
     4261 cgggcaatat gtcgccgtca aggataacgg tccgggcata ccggaagacg aaattcccgt
     4321 cgttctctca gccttcggcc agggttccat cgccataaag agcgccgaac agggcactgg
     4381 ccttggcctg ccgatcgtgc aggggcttgt cgccatgcat ggcggcgaat tcgaactgca
     4441 ttccaaacta cgcgaaggca ccgaggctat cgccatcttc ccgctaagcc gggtgatgga
     4501 agagttgccg gcgctgccca tgaaggcggt ggcaggcggc cgacgccgct agagaatttc
     4561 cggcaaaagt gtgaacggtt ttgcgtccgg aaatgcggcc aaaacaaaaa gatagtgggt
     4621 cggcggatca atgctcgcgt acggccgccg ccatgcccga actgcaaagg gcggctgcct
     4681 tcacgatgcc tgcgccaagc gctgccccca cgccctcgcc atgatcgaga gccaggtcga
     4741 acagcggcgc atcgaggccg agcttttcac cagccgccgc aagggccggt aacgccggca
     4801 ggctggcaag caggcaatgg ctgattgcat ccggattgac ggcatgcagc accgctgacg
     4861 ccgccaatgc ggcaaaaccg tcgatcaaca ccgggatttt ctgcatccgc gccgcaagta
     4921 tcgcgccggc aatcgcggcg aattcgcgcc cgcctacccg gcgcaacgcc tccagcgggt
     4981 ccttgagact attgccgtgg aaggccagcg ccgcatctac gatgtctgcc ttccgcacca
     5041 tcatgtcggc atccgcgccg gaccccttgc cgacccagtc gctgcccttg ccgccgagga
     5101 tagccgccag caaagc
//
LOCUS       DASYCS010000458        15381 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_137987,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000458 DASYCS010000000
VERSION     DASYCS010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 15381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15381
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137987"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VGM46_14225"
     CDS             <1..193
                     /locus_tag="VGM46_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019996791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY4193799.1"
                     /translation="ILLDTAKSSRDSLRARALFAERLAHKAHSSWSWKGYGDILATLG
                     QDERALEARARAAELVSGN"
     gene            640..1794
                     /locus_tag="VGM46_14230"
     CDS             640..1794
                     /locus_tag="VGM46_14230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4193800.1"
                     /translation="MAGISTQREARRGDKIISGDQRAAATAAAGIGSSISISHVRKSY
                     GNFVALDDANFDIAPGEFITLLGPSGSGKTTLLNIIAGFQNIDKGEVLVDGKPVLHVA
                     PHRRGFGMVFQSYALFPNMTVTQNVAFPMRMAGVDRETTDRRVAETLEMMRLSDHAAK
                     MPSQMSGGQQQRVAIARAIVMRPRVVLMDEPLSALDRRLRESIQIEIRELHRTIGSTI
                     LFVTHDQGEALTMSDRIAVLDAGKIIQIASPTEIYRHPATRFVASFVGESNLIDVELV
                     QRRGTRLTLKNRAGFTFEAGSRNEDAGSTATVLVRPERIAMSDTPAPNSVPITVTSAL
                     FLGELLRVECEMEGGGKLLIRCTDTQSRAMPNIGDKLYASWGADDCWVLS"
     gene            1797..3590
                     /locus_tag="VGM46_14235"
     CDS             1797..3590
                     /locus_tag="VGM46_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY4193801.1"
                     /translation="MAQSSVAMRHPARRSYWNLLKNPSILLLPATLFLAVIYVWPLLD
                     LFRLSVGGPTWTSHFERVFSIPLYWQSLVRTMEISITVSALCLFFGYPTALLIHRSQG
                     IVKVMVATAVVLPYFIAILIRTYAWMVLLGRNGPLNKLAIWLGILDQPAELLFNRGSV
                     LIGMTAVLVPLMVLSIYSSVSRIDGNLTRAALASGAGPIAVFWRVLLPLTFPGMGAGF
                     LLVFVSGLGFFITPTLLGGPGDQMFAMHITQQADFLTSEGFLQALAVVLLMITLVVVG
                     IAGHFLGFEFIWGGEKLSGVTPSASKTDRGGRPASGSAVGNLFADAVGWPLLRLLGKL
                     PPSAGTWLVCVMGGFVILALIAPIVIVSIISFSKASFLTFPPPGFSLRWYSKFFSDSD
                     WMSAFTTSLSVAAISAAIAVAFGTSAAIGIVRSSLKAKTAIMLLLVSPIIVPPVVLGL
                     SLYSLFLYFDLIGSRIGLAAAHAIGGIPLVVVIIAASLQGVDRKLEQAAAIHGASPLT
                     VLRKVTLPAIAPGLAAASFFAFLHSFDELVLSLFLSSAHLKTLPLRLWADVNYQLNPV
                     LAVVSTLEVVLVVGGILIARPVLAGSRKAAR"
     gene            3651..4733
                     /locus_tag="VGM46_14240"
     CDS             3651..4733
                     /locus_tag="VGM46_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY4193802.1"
                     /translation="MQVKLKEMIAGGMLASVMMLTGAGGAAAQSNEVIMQDPGGQYGE
                     ALRSIMYDPFEKETGIKVVTVQEARSGPKIKAQGSGKALWDLTFIFDQETKLLGDCCL
                     MDIDYSKLSESAQKTLATMPDNLKRKKGVALQVIGVAMAYNTEKYKTAPKDWADFWDV
                     KTYPGKRCMPGWSRFVFEAALMADGVDKDKLYPIDMERALKKIAEIKPYVSKWWTTAA
                     QAPQLMLDGEADMCMAYAGRINDLVVNEGAPVDMTWNQGFVYYDFFSIPKGAPNPENA
                     LKLLSYRLDAKRGAELSTAYPVPVPSPLVFQAADPKVSRLWANNPDNVKQAIQWSADY
                     WGAPSPDGKSTNEEYAQEKLNALLAE"
     gene            complement(4829..5806)
                     /locus_tag="VGM46_14245"
     CDS             complement(4829..5806)
                     /locus_tag="VGM46_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEY4193803.1"
                     /translation="MRKVCLIATGGTIASQRDRGNGHVTASITGPGLRAMLPHPLDDI
                     DLTVDDFGKINSFAIDLPFSFSLARRISDRLAEGFDGVVVTHGTDTMEESVFMADLVV
                     DSDKPVVFTGAQHAADDANPDGPQNLVDSLRLAASEQVMGLGAMICFDQEFHSARDVI
                     KSHTSRTDTFVSAEHGKLGEVDGDRIVVHRRPALRRTYKPGQVASDVELIKLAMGTSD
                     RYLRFAADNGAQALVLEGFGRGNAPPAVTAAAAEVIKKGIPVIMTSRCQRGRVKPIYG
                     NGGGKTLAEAGVIFAGDLSGQKARVLLSVLLGLGLSENQIRSEVEYLGG"
     gene            complement(5905..6930)
                     /locus_tag="VGM46_14250"
     CDS             complement(5905..6930)
                     /locus_tag="VGM46_14250"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY4193804.1"
                     /translation="MPRDPINPERVTMRSIADMAGVNVSTVSRVLRQRPGPSDSEAAR
                     QIRLLANQFGYRPDVWAASLRSRRTHAVGVIVTRLTDVVMAAVYESIEQHAQSRGYLA
                     IGGSSGDDVEQQRRRIEHYLDRRVDGLLIGDAHLEAPFLDELSQRNVPFVLFNRSSGN
                     HPSVTWDDEMGGAIVARHFLDLGHKRIAIVNGPTYASSGVVRAKGFVDALAKEGVVLP
                     PEMIINAGFDASAGRRAAVELLAMRPRPTAIFAVNDYSAIGVMGVIREQKLVVGRDIA
                     VVGYNDITIANDLLVPLSSVRVPIDVMGRTAVEMILARMDGEVVNSRRLVPYLVVRDS
                     SRRHPET"
     gene            7202..8251
                     /locus_tag="VGM46_14255"
     CDS             7202..8251
                     /locus_tag="VGM46_14255"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY4193805.1"
                     /translation="MRLHHTIATACFALACSTSIGGIGHANAAERKVIGFAVPVLANP
                     FWKQEVDFAKRVADELGGDLIVEDANQDEAKQLQIMDNFASRKVDAIITAPVTTAVAP
                     ALIKAAGRANIPIFFAERTPGFEPDLEKYPQYIGFVGADSHQGALATAKALYDAGARN
                     IVATTGTKGSSIAEDRIGGLKQFASEHPDFKVLQVQYGVELREDGIKATENYLSAYPG
                     PGFDGIWNYDDGAAIGAAAALKQANLTGKVLVTGFDAEADGAQAIKAGDILADSGGQF
                     IDGGFAYIMAFDYLNGHKPKKPYVVMDYLLVTKDNADKYIEQFGSGFPPYDVKKMSVT
                     FNPDASTDDYKIAIQ"
     gene            8316..9830
                     /locus_tag="VGM46_14260"
     CDS             8316..9830
                     /locus_tag="VGM46_14260"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEY4193806.1"
                     /translation="MSDTIRLVLKGIRKTYDASTVLDLSNSEDVVFRQGEIHGLAGEN
                     GAGKSTLVKVVCGLVSPTIGSMELDGKPYAPTNLAEARAAGVEIVLQEPGLVPGLTVA
                     DNLFLGRERIYSRFRILNGRRRDELARNALGRVGATTPINKRAGDLDLESQKFVELAR
                     ALAFEPRVLVIDEMSASLTKQGVVRMSRVLREAAAQGTAILYISHYLEETFDLCQQVT
                     VLKDGRHVQTLATDKLDESHLQTLMVGRSLVSELYHEGSRAAPAELAAPALKISGLKA
                     GRAVRDFSLDVKAGEIVGLGGLVGCGSEEIAHAIFGVGRMADGEITLSGNPYRPSSSR
                     HAIDSGVGFVSGDREANDLLLQATIQDNIALPTLPMRSNWGLTSRRGDAAEASKVIRE
                     LSIACRGPADYPAKLSGGNRQKVVLGKWMLRRPNVLVLQNPTRGVDVGAKLLIYSTLR
                     RLAGMGTAILLISDELNELRGMSDRIVIMRRGMIAGWFDASANPSEEELVACMV"
     gene            9842..10813
                     /locus_tag="VGM46_14265"
     CDS             9842..10813
                     /locus_tag="VGM46_14265"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4193807.1"
                     /translation="MAQTTEPSAGTRNDLRTSGREIARKFIPFVALLLLIMVFSAAAP
                     DRFATLDNVRTVLVQTVVLAIVAFGSTLVIIAGSIDLSVGSVAALASMVGASVAQDYN
                     GVLGLIAAIIVGALAGGVNGVGLAYFKVPSFIMTLGMLSVARGLTLLYSGTQPIMTPG
                     VWDWMGVAPGIYVPGIVVFIVTFCLFRFTAFGRYVSAVGGQEKVASMSGVRVNQVKML
                     VFVISGITAAIGGVVLSSRVGAATPTAGTGMELTVIAAVVLGGTPLTGGMGSLVNTIV
                     GALIINILLNGLIIVGIGAELQIIAQGLVLVGAVLVSLDRRKIGIIK"
     gene            10829..11902
                     /locus_tag="VGM46_14270"
     CDS             10829..11902
                     /locus_tag="VGM46_14270"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: protein motif:HMM:NF006048.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HEY4193808.1"
                     /translation="MKNYSIAVIPGDGIGAEVMPPALDILDTVAGRHGFGLSYTHLPW
                     GCEFYARHGRMMDEDGLKQLAGHDAIFLGAVGYPGVADHVSLWGLLIPIRRAFRQYIN
                     LRPIRLLEGVKGPLSGKKIDMLIVRENNEGEYSEAGGRLYRGTADEIAIQEAVFTRRG
                     IERAAGFAADLAKTRSGSMVAATKSNGIIHTMPFWDEVCGEVAKDKGVGIAFRHIDAL
                     AADFVLHPERLDVVVASNLFGDILSDLGAAVAGSIGIAPSANLNPEREHPSMFEPVHG
                     SAPDIAGQGIANPVGQIWSAAMMLDHLGEKAAAAEIMGALETVLATSQIRTRDLGGEA
                     STTEFAQEIKGRLGAKTTASASQ"
     gene            11921..12676
                     /locus_tag="VGM46_14275"
     CDS             11921..12676
                     /locus_tag="VGM46_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY4193809.1"
                     /translation="MTQAVIITGAGIGIGRATAFAFAKAGYHVAVTDVLEEDGRKVAA
                     DIVASGGLAAFHKLDVTSTEQVNQVVAGVVDKFGAPHAVVANAGIALREPFEEMSDER
                     WQRTLDIDLNGVMRLFRASAPSMRGAGRGSMIAMSSIMGIAYGWDEHVHYSAAKSGVI
                     GLVRGLAVELAPEGVRVNGIAPGYIRTAQALSTDHSLGAKGLEAASGYIPMRRVGEPE
                     DIADVAVFLGSEQSRYITGQVITVDGGLLVGRF"
     gene            12741..13643
                     /locus_tag="VGM46_14280"
     CDS             12741..13643
                     /locus_tag="VGM46_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HEY4193810.1"
                     /translation="MAKKIYCAFGTDIDSVAGWIGSYGGGDSPSDIQRGIFAAEVGVP
                     RLLKLFRKYDLPTTFFIPGHSLETFPKEMKMIVDAGHEIGAHGYLHENPIAMTAQQEE
                     DVLAKSIELIEKLSGSVPRGYVAPWWEMSASTADLLQKYGFSYDHSQGYRDFQPFYAR
                     VGDEWNVIDYTKKAKDWMHPLKHGKEIDLVDIGANWYVDDLPPMMFMKKAPNSHGFVN
                     PRHIEEMWRDQFDWVYREMDYAVFAFTIHPDVSGRPQVLLMLERMIEYISGHTGVEWA
                     TFDKIANDFRARYPFDGGKRPEVI"
     gene            13647..13940
                     /locus_tag="VGM46_14285"
     CDS             13647..13940
                     /locus_tag="VGM46_14285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193811.1"
                     /translation="MCSACGFPSLPGHWTDAGARSPGSRLRLRFTRLTMINRLLAPYR
                     LRAHDDGSTPGLQLMAPDGERVLVPDLEALWVQAERLAGTPIDPLSPRFLGDA"
     gene            13933..14922
                     /locus_tag="VGM46_14290"
     CDS             13933..14922
                     /locus_tag="VGM46_14290"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CobW family GTP-binding protein"
                     /protein_id="HEY4193812.1"
                     /translation="MRKGARSDGRLRLTIIAGFLGAGKSTWLRHQLHENTFGSVHVFV
                     NEAAEMPVDNLLLGKADRISVLAGGCACCEGRATLVEALRNTCQEQDGADAQPIDRIL
                     LETSGLADPGAITKALSEDPILARRLVLDETIVLVDALHGLAQLNEERLGRRQVEAAD
                     RLIITKPGSVAASQLARMASTLRQLNPMALVESAERGTSFALQTVPDAEPYPLADIAD
                     DAMPIKPCRLDISGEYGWAAFSVWLSALLAARGEQIMRVKGVVTTADGRLLLQSVRKQ
                     VQPPEMLPEMDADSSVQGPRDNIIIFIGRGIDDALLTSAWARFTQDNPGQMRR"
     gene            complement(15153..>15381)
                     /locus_tag="VGM46_14295"
     CDS             complement(15153..>15381)
                     /locus_tag="VGM46_14295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193813.1"
                     /translation="AAIMKAEAEGRDIERKDDEAYNKKVEAEWVKEGKTVTHPDIKKF
                     QDIAYSIYPQFYDKVGGKELVDRIRKIGDEM"
BASE COUNT         2941 a         4818 c         4655 g         2967 t
ORIGIN      
        1 gatcctgctc gatacggcca agtccagccg ggattcgctg cgcgctcgcg cgctgtttgc
       61 cgaacgcctc gcccacaagg cccattcgag ctggtcgtgg aaaggctatg gcgacatcct
      121 tgcaacgctc ggccaggacg aacgcgcgct ggaagcacgg gcgcgcgcgg cggaactggt
      181 ctcgggcaac tgacgcagcg gcgggcgcaa gacttgcgcc cgccttcagc ggtctgatct
      241 cgccaggtac agcaagcccg gctatgaaac gcctcggctg taacggggca aaggccgggt
      301 cggcggagaa ctacttccgg gcatcgccat gaaagcgtcg gcacgatcca tcggaagcgc
      361 ggcaaccatg ccgcccccga cattgagcga gccggttcac gcaaccttga tcctacgcga
      421 gcacgttcat cgagccgcga aggctaagct ctcaacctca aaatttcgtt ccgatgcatg
      481 gaactcgatt acgaaacgcc gcaacgaaga cgtccgcgcc cggtcaatcg acagcctgtc
      541 attcgatgca aagctcacat gaacgcttga caagcaggag atcggcaata atctaattat
      601 gaaatctcgt tctacatctt ggaacgctag ggaggaagca tggctggcat ctcaacgcag
      661 cgcgaagcgc gccgcggcga taagattata tcgggggacc agcgcgccgc cgccacagcc
      721 gccgccggga taggcagcag catcagcatt tcccatgtcc gcaaaagcta cggcaatttc
      781 gttgccctgg acgacgccaa tttcgatata gcgccgggcg aattcatcac gcttttgggg
      841 ccgagcggca gcggcaagac cacgcttctc aacatcatcg ccggcttcca gaacatcgac
      901 aagggcgaag tcctggtcga tggcaagccg gtgctgcatg tggcgcccca ccggcgcggc
      961 ttcggcatgg tgttccagag ctatgcgctg tttcccaata tgacggtcac gcagaacgtc
     1021 gcctttccga tgcgcatggc cggcgtcgac cgcgagacga ccgacaggcg cgtcgccgaa
     1081 acgctggaaa tgatgcgctt gtcggaccac gccgccaaga tgccgagcca gatgagcggc
     1141 ggccagcagc agcgcgtggc gatcgcccgc gccattgtca tgcggccgcg cgtcgtcctg
     1201 atggacgaac cgctcagcgc gctcgaccgg cggctgcggg aatcgatcca gatcgagatc
     1261 cgcgaactgc accgcaccat cggcagcacg atcctgttcg tcacccacga ccagggcgag
     1321 gcgctgacaa tgagcgaccg catcgccgtg ctagatgccg gcaagatcat ccagatcgcc
     1381 tccccgaccg aaatctatcg ccatcccgcg acccgtttcg tggcttcctt cgtcggcgaa
     1441 tccaacctca tcgacgtcga actggtgcag cggcgcggga cccggcttac cctcaagaac
     1501 cgcgcgggtt tcaccttcga ggccggaagc cgcaatgaag acgcgggcag caccgccacc
     1561 gtgctcgttc gccccgagcg catcgccatg agcgacacgc cggcgcccaa ttcggtgccg
     1621 atcacggtca cttcggcact cttcctcggc gagcttttgc gcgtcgaatg cgagatggag
     1681 ggcggcggca agcttcttat ccgctgcacc gatacgcaat cgcgcgccat gcccaatatc
     1741 ggcgacaagc tttatgcaag ctggggcgcg gatgattgct gggtattgtc atgacgatgg
     1801 cgcaaagcag cgtcgccatg cgccaccctg cccgcaggtc ctattggaac ctgctgaaga
     1861 acccttccat cctgcttttg ccggccacgc tgttcctcgc cgtcatctat gtctggccgt
     1921 tgctggacct cttccgcctc agcgtcggcg gcccgacatg gaccagccat ttcgaacggg
     1981 tcttttccat cccgctctac tggcagtcgc tggtgcgcac gatggagatt tccatcacgg
     2041 tctcggccct gtgccttttc ttcggctacc cgaccgcgct tttgatccac cgttcacagg
     2101 gcatcgtgaa ggtgatggtc gccaccgccg tcgtccttcc ctatttcatc gccatcctga
     2161 tccgcaccta tgcctggatg gttctgcttg gccgcaacgg gccgctgaac aagctggcca
     2221 tatggctggg cattctcgac cagccggcgg aactgctctt caaccgcggc agcgtattga
     2281 tcggcatgac ggccgtgctc gtgccgctga tggtgctcag catctattcc agcgtctcgc
     2341 gcatcgacgg caacctgacg cgcgccgccc ttgccagcgg cgccggcccg atcgccgtgt
     2401 tctggcgcgt acttctgccg ctgacctttc ccggcatggg cgccggtttt cttctggttt
     2461 tcgtcagcgg tctcggcttc ttcatcacgc caacgctgct tggcggcccc ggcgaccaga
     2521 tgttcgccat gcatatcacc cagcaggccg acttcctgac cagcgaaggc ttcctgcaag
     2581 cgcttgccgt cgttctcctg atgatcaccc tggtggtggt cggcatcgcc ggccatttcc
     2641 tcggtttcga attcatctgg ggcggcgaga agctgtcggg cgtcacgcct tccgcttcga
     2701 agaccgacag gggcggccgg cccgcttcgg gcagcgccgt cggcaacctg tttgccgatg
     2761 cggtcggctg gccgctcctg cgtcttctgg gcaagctgcc gccaagcgcc ggcacctggc
     2821 tggtatgcgt gatgggcggc ttcgtcatcc tggcgctgat cgcgccgatc gtgatcgtca
     2881 gcatcatctc gttcagcaag gcgagcttcc tgacctttcc gccgccgggc ttttccctgc
     2941 gctggtacag caagttcttc tcggactcgg actggatgtc ggctttcacc acctcgctca
     3001 gcgtggcggc catttcagct gccatcgccg tcgccttcgg cacctcggct gcaatcggca
     3061 tcgtacgcag ttcgctgaag gccaagacgg cgatcatgct gctgctggtc agcccgatca
     3121 tcgtgccgcc ggtcgtgctt ggcctgtcgc tctacagcct cttcctctat ttcgacctga
     3181 tcggatcgcg catcggcctt gcggcggcgc atgccatcgg cggcatcccg ctcgtcgtcg
     3241 tcatcatcgc ggccagcttg caaggcgtcg accgcaagct ggaacaggcg gccgcaatcc
     3301 atggcgcgtc gccgctgacc gtgctgcgca aggtgacgtt gccggccatc gcgccgggtc
     3361 ttgccgccgc cagcttcttt gccttcctgc actctttcga cgaactcgtt ttgtcgctgt
     3421 tcctgtcgag cgcgcatctg aaaaccctgc cccttcgcct ttgggcagac gtcaactacc
     3481 agctcaaccc ggtgctcgcg gtcgtctcga cgctggaagt ggtgctggtc gtcggcggca
     3541 ttctgatcgc caggcccgta ctggcaggct ccaggaaagc cgcaaggtga atgccgcaaa
     3601 cgcataccgg actatcaagt ggagaaactg acatgctgag gagacgtgtt gtgcaagtta
     3661 aactgaagga aatgatcgcc ggcggcatgc tggcttcggt catgatgttg accggcgccg
     3721 ggggtgcggc ggcacagagc aacgaagtga tcatgcagga tccgggcggc caatatggcg
     3781 aagcgctccg ttccatcatg tacgatcctt tcgagaagga aaccggcatc aaggtcgtca
     3841 ccgtgcagga agcccgcagc ggccccaaga tcaaggcgca gggttccggc aaggcgctgt
     3901 gggacctgac cttcatcttc gaccaggaaa ccaagcttct cggcgattgc tgcctgatgg
     3961 acatcgacta cagcaagctt tcggaaagcg cgcaaaagac gctggcgacc atgccggaca
     4021 atctgaagcg caagaagggc gtcgcgttgc aggtcatcgg cgtcgccatg gcctacaata
     4081 ccgagaagta caaaaccgcc ccgaaggact gggccgactt ctgggacgtg aagacctatc
     4141 ccggcaagcg ctgcatgccc ggctggtcgc gcttcgtgtt cgaagcggcc ctgatggccg
     4201 acggcgtcga caaggacaag ctctacccca tcgacatgga gcgcgcgctg aagaagatcg
     4261 ccgaaatcaa gccttatgtc agcaagtggt ggaccacggc cgcccaggcc ccgcagctga
     4321 tgctcgacgg cgaagccgac atgtgcatgg cctatgccgg ccgcatcaac gacctcgtcg
     4381 tcaatgaagg cgctccggtc gacatgacgt ggaaccaggg cttcgtctat tacgacttct
     4441 tctcgatccc caagggcgct cccaatccgg aaaacgccct gaagcttctg tcttaccggc
     4501 tggacgccaa gcgcggggca gagctttcca ccgcctatcc ggttccggtt ccctcgccgc
     4561 tggtcttcca ggccgccgac cccaaggtca gccgcctgtg ggccaacaac ccggacaacg
     4621 tcaagcaggc catccaatgg agcgccgact actggggcgc gccctcgccg gacggcaagt
     4681 ccaccaacga ggaatacgcc caggaaaagc tgaacgccct gctggctgag taaaaaaaca
     4741 aggctcaggc cgggaaaccc ccggcctgag ccattcatga tgcccgatta aggcacatcg
     4801 aaaaagccgc ccgccgcggc tttttctgtc aacctccgag atactcgacc tcggaccgta
     4861 tctgattttc gctcaagccc aatccgagca gaaccgacaa taatacgcgc gctttctggc
     4921 cgcttagatc tccggcaaat atcacccccg cttcggcaag ggtcttgccg ccgccattgc
     4981 cgtagatagg cttgacacgt ccgcgctggc agcggctggt catgatgacc ggaatgcctt
     5041 ttttgatcac ctcggccgcg gctgcggtga cggctggcgg ggcgtttccc cgaccgaatc
     5101 cctccagcac cagcgcctgc gccccgttgt cggctgcaaa gcgcagatac cggtcgctgg
     5161 ttcccatggc cagcttgatc aactcgacat cgcttgcgac ctggcctggt ttgtaggtac
     5221 gtcgcagggc gggccggcga tggaccacga tccggtcacc gtcgacctcg cccagcttgc
     5281 cgtgttctgc ggacacaaaa gtgtcggtcc gcgacgtatg ggattttatg acatcccgtg
     5341 cggagtggaa ttcctggtcg aagcaaatca tggcgccaag tcccataact tgttcggacg
     5401 cggcaaggcg cagggaatcg acgagattct gcggcccgtc cggatttgcg tcgtcagcgg
     5461 catgctgagc accggtgaag acgacaggct tgtcggaatc aacgacgaga tccgccatga
     5521 acacgctctc ttccatcgtg tcggtgccat gggtgacgac gacgccgtcg aaaccctccg
     5581 caaggcggtc agaaatacgc cgtgccagcg agaagctgaa aggaaggtcg atggcgaaac
     5641 tgttgatctt gccgaaatca tcgaccgtca ggtcgatatc gtccagggga tggggcaaca
     5701 tggcgcgcaa ccccggaccg gtgatacttg cggtaacgtg cccattgccc cgatcccgct
     5761 gcgaagcgat cgtgcctcct gtcgctatca ggcatacttt ccgcattcat agtcctcttg
     5821 gcatgtgatt tgacgaattc ttgcagtcat gacgaacctt gccgcgcggc agaccaggcc
     5881 ggcgatggtc ataaagatcg atagctatgt ttcggggtga cgccgcgagg aatcgcgaac
     5941 gaccaggtaa ggtacaaggc gtcgggaatt gacgacttct ccatccatgc gggcgaggat
     6001 catctcgaca gcggtgcggc ccatgacatc gatcggcacg cgtacgctcg acagcgggac
     6061 cagaaggtcg ttggcgatgg tgatgtcgtt atagccgaca accgcgatat cccttccgac
     6121 cacgagcttt tgctcgcgga tcacacccat gacaccgatg gccgaatagt cgtttacggc
     6181 gaagatcgcg gttggccgtg gccgcatggc gagcagttcg accgcggccc tgcgccccgc
     6241 cgaggcgtcg aagccggcat tgattatcat ttcgggcggc agcaccacgc cttcctttgc
     6301 cagggcgtcg acaaagccct tcgccctgac gacgcccgaa ctggcatatg tgggaccgtt
     6361 gacgatcgca atgcgcttat ggccaaggtc aaggaagtgc cgcgcaacga tcgcaccgcc
     6421 catctcgtcg tcccatgtca cagacgggtg gttgcccgac gagcggttga aaagaacgaa
     6481 tgggacgttg cgctgcgaga gttcatccag gaacggggct tcaaggtgtg catcgccgat
     6541 aagcagcccg tcgacacgcc ggtcgagata atgctcgatc cttcgccgct gctgttcgac
     6601 gtcgtcgccc gatgagccgc caatcgcaag gtagccgcgt gattgggcgt gttgctcgat
     6661 cgattcgtag accgccgcca tgaccacgtc cgtgagacga gtgactatca cgccgaccgc
     6721 gtgcgtgcgg cggctgcgaa ggctggctgc ccacacatcg ggccgatagc cgaactggtt
     6781 ggccagaagc cttatctgcc tggcggcctc gctgtccgaa ggtcccggac gctgtcgcaa
     6841 gacgcgcgag accgtggaaa cgttgacgcc tgccatgtcg gcgatcgagc gcatggtcac
     6901 ccgctcgggg ttgattggat ccctaggcat gcgtcctcac ttcacccaca gggccatggc
     6961 ggtatcgcca atcgccaaac ggcacgtttt cccggcacaa gtcaccaccg catcggccct
     7021 cgtccatagc cggcaccgct atcgtttcgg atcgccagcg tcgctatctg caagttcact
     7081 tgaacaccct tgacacgaat aagccaaccg tgatcttaaa gagatgcaaa cggttgcgca
     7141 atcgtttgtt gagttatcat tttaaccggc gcaacgccgg ttgtaacgtg ggaggaagcg
     7201 gatgcgcctg catcatacaa tcgcaacggc ttgttttgcc ttggcctgct caacttcgat
     7261 cggcggaatt gggcacgcca acgctgcgga gagaaaagtc atcggctttg ccgtgccggt
     7321 attggccaat ccgttctgga aacaagaagt cgacttcgcc aagcgcgtcg ccgacgaact
     7381 tggcggcgac ctcatcgtcg aggacgccaa tcaggacgaa gcaaagcagc ttcagatcat
     7441 ggacaatttc gcgtcgcgca aggtcgacgc aatcatcacg gcaccggtga cgaccgccgt
     7501 tgcgcccgcc cttatcaagg ctgccggccg cgcgaacatt ccgatcttct tcgcggaacg
     7561 cacgccgggc ttcgagcccg acctggagaa gtacccgcaa tatatcggct tcgtcggggc
     7621 cgacagccat cagggtgcat tggcgaccgc caaggctctt tacgatgccg gcgcgcgcaa
     7681 tatcgtagcc accaccggca ccaagggcag ctcgattgcg gaagaccgca tcggtgggct
     7741 gaagcagttc gccagcgaac acccggattt caaggtcctc caggtccagt atggtgtcga
     7801 attgcgtgaa gacggcatca aggcgacgga aaactacctc tccgcctatc ccggccccgg
     7861 cttcgacggc atctggaatt acgacgacgg cgcggcgatc ggcgcggcgg ctgccctgaa
     7921 gcaggccaac ctgaccggca aggtgctggt caccggcttc gatgccgaag cggatggcgc
     7981 gcaggcgatc aaggcaggcg atattctcgc cgattccggc ggccagttca tcgacggcgg
     8041 cttcgcctac atcatggctt tcgattatct caacggccac aagccgaaga agccatatgt
     8101 ggtcatggac tatctgctcg tcacgaaaga caatgccgac aaatatatcg agcagtttgg
     8161 ttcgggcttt ccgccctatg acgtcaagaa gatgtcggtg acgttcaatc cggatgcgtc
     8221 gaccgacgat tataagatcg cgatccagta aacaatcgaa aagggcgacc cggcgcgccg
     8281 cgtcgggtcg cccatgtctg tcggaggggc ggaagatgtc ggatacgatt cgtctggtct
     8341 taaagggcat acgaaagact tacgatgcct cgacggttct ggacctgtcg aactccgaag
     8401 acgttgtctt tcgccagggt gaaatccacg ggctcgccgg tgaaaacggc gcaggcaaat
     8461 ccaccctggt gaaggtcgta tgcggcctcg tgtcgccgac tatcggatcg atggaactcg
     8521 acggcaagcc atacgcgccc acgaacctcg ccgaagcgcg cgcggcgggc gtggaaatcg
     8581 tgctccagga gccgggactc gttccgggat tgacggtagc cgacaatctg tttctcgggc
     8641 gcgagcgcat ttattcccgt ttcaggattt tgaatgggcg gcgtcgtgac gaacttgccc
     8701 gcaacgcttt gggaagagtt ggcgcgacga ccccgatcaa caagcgggcc ggcgatctcg
     8761 accttgaatc gcaaaaattt gtggaactcg cgagagcgtt ggcttttgaa ccccgcgtcc
     8821 tggtaatcga cgagatgtcg gccagcctga caaaacaagg tgtggtgcgc atgtcgcgcg
     8881 tgctgcgcga ggcggcggct cagggcaccg cgatccttta catctcccac taccttgaag
     8941 agacattcga cctttgccag caggtcaccg ttcttaagga cgggcgccat gttcaaacat
     9001 tggccaccga taaacttgat gaaagccatt tacagaccct catggtgggc cgctcgcttg
     9061 tcagcgagct ttatcatgag ggcagccgtg ccgctccggc ggaacttgcg gcgcccgcct
     9121 tgaaaatatc tggcctgaaa gcgggccgcg cggtccgcga tttcagtctc gacgtcaaag
     9181 ccggagaaat tgtcggcctc ggcgggctgg tcggctgcgg cagcgaggag atcgcgcatg
     9241 ccatcttcgg cgtcgggcgg atggcagatg gtgaaatcac gctttccgga aacccgtatc
     9301 gcccttcaag ctcccgacac gccatcgaca gcggtgtagg cttcgtctcg ggcgaccgcg
     9361 aggccaacga cctgcttttg caggcgacga tccaggacaa tatcgcgctg ccgaccttgc
     9421 cgatgcgctc caattggggt ctcacgtcca ggcgcggcga tgcggcggaa gccagcaagg
     9481 tcatccggga gctttccatc gcctgccgcg gcccggccga ctatcctgca aagctgagcg
     9541 gcgggaaccg gcagaaggtc gtgcttggaa agtggatgct gcggcgtccc aatgttctgg
     9601 tgctgcaaaa tccgacccgc ggcgtcgacg tgggcgccaa attactcatc tacagcacgc
     9661 tgcggcgact ggccggcatg ggcaccgcca ttttgctgat aagcgacgaa ctgaacgagt
     9721 tgcgaggcat gagcgaccgc atcgtcatca tgcggcgggg catgatcgcc ggttggttcg
     9781 acgcctcggc caatcccagc gaagaagaac tcgtcgcatg catggtttga aggaagaaga
     9841 tatggcgcag acgactgaac caagtgccgg tacgcgaaac gacctgagga ccagcgggcg
     9901 tgagatcgcg cgcaagttca taccttttgt cgcgttgttg ctgctgatca tggtcttcag
     9961 cgcggcagcg cccgaccgct tcgcgacgct cgacaatgtc aggaccgtcc tggtgcagac
    10021 cgtcgttctg gcgatcgtcg cattcggttc caccctggtg atcatagcgg ggtccatcga
    10081 cctgtcggtc ggctcggtcg ccgccctcgc cagcatggtc ggggcgagcg tcgcccagga
    10141 ttacaacggc gtactcggcc tgatcgcggc catcattgtc ggcgcgcttg caggcggagt
    10201 gaacggcgtc gggttggcct atttcaaggt cccctcgttc atcatgacac tgggcatgct
    10261 ttcggtggcg cgtgggctga cgctgcttta ttccggcacg caaccgatca tgaccccggg
    10321 agtctgggat tggatgggtg tcgcgcccgg catctatgta cccggcatcg tcgtcttcat
    10381 cgtcacattc tgcctgttcc gcttcacggc gtttggccgc tatgtctcgg cagtgggcgg
    10441 ccaggagaag gtggcctcca tgtcaggggt ccgcgtcaac caggtcaaga tgctggtctt
    10501 cgtcatctcc ggcatcaccg ccgccattgg cggcgtcgtc ctttcatcgc gcgtcggcgc
    10561 cgccacgcca acggccggaa ccggcatgga actgacggtg attgccgccg tcgtgctcgg
    10621 cggaacgcca ttgaccggcg gcatgggcag ccttgtgaac acgatcgtcg gcgcgctcat
    10681 cataaatatt ctgctcaacg ggttaatcat cgtcgggatc ggcgccgagt tgcaaatcat
    10741 cgcacagggc ctcgtcctgg tcggggcagt gctcgtgtcg ctcgaccgca ggaaaatcgg
    10801 catcatcaag taggcggacg cagcgatcat gaagaactat tccattgcag tcattcccgg
    10861 cgacgggatc ggcgcggagg ttatgccgcc ggcgctcgat attctcgata ccgtcgccgg
    10921 ccgccacggc ttcgggctgt cctacacgca cttgccatgg ggctgtgaat tctacgctcg
    10981 ccatggccgc atgatggacg aggacggcct gaagcagctt gccggccatg acgctatctt
    11041 ccttggcgct gtcggctatc cgggggtggc cgaccatgtc tcgctgtggg gactgctcat
    11101 ccccatacgc cgcgcctttc gccagtacat caacctgcgc ccgatccggc tcctggaagg
    11161 cgtgaagggg ccgctttctg gcaagaaaat agacatgctc atcgttcggg agaacaatga
    11221 gggcgaatat tccgaagcgg gcggccgtct ctatcgcggc accgccgatg aaatcgcaat
    11281 acaggaagcg gtcttcaccc gtcgcggcat cgaacgcgcg gccggctttg ccgcggatct
    11341 ggccaagacc cgatccggct cgatggtggc cgccaccaag tccaatggca tcatccacac
    11401 catgccgttc tgggatgagg tttgcggtga agtagccaag gacaagggtg tgggcatagc
    11461 cttccgccac atcgatgcgc tggccgccga tttcgttctc catcccgagc ggctggacgt
    11521 cgtggtcgcc tccaatctgt tcggcgacat tctgagcgat ctcggcgccg ccgtcgccgg
    11581 cagcatcggc atcgcgccat ccgcgaatct caatcccgag cgcgagcacc cgtccatgtt
    11641 cgagcctgtc catggatcgg cgcctgacat tgccggacag ggcatagcca atccagtcgg
    11701 gcaaatctgg tccgccgcca tgatgctcga ccaccttggc gagaaagccg cggcggccga
    11761 gatcatgggc gcgctggaga cggttctggc cacgtcgcaa attcgcacga gagatctcgg
    11821 cggcgaggct tcaacgacgg aattcgcgca ggaaatcaag ggccgcctcg gcgccaaaac
    11881 aaccgccagc gccagccagt agcataagag gagcatacgc atgacacagg ctgtaatcat
    11941 cacgggggcc gggatcggaa tcggtcgggc gacggcattc gctttcgcca aggctggcta
    12001 ccatgtcgcg gtcacggacg tgttggagga ggatggccga aaggttgccg ccgacatcgt
    12061 ggcgtcgggc ggattggccg ctttccacaa gctcgatgtg accagtaccg agcaggtcaa
    12121 tcaggtcgtg gcgggcgtgg tcgataaatt tggcgccccg catgccgtcg tcgccaatgc
    12181 aggcatcgcg ttgcgcgagc cattcgaaga gatgagcgac gagcgctggc agcgcacgct
    12241 cgacatcgac ctcaacggtg tcatgcgcct gtttcgcgct tcggcgccga gcatgcgcgg
    12301 cgccggtcgt ggcagcatga tcgcgatgtc gtcgatcatg ggtattgctt acggctggga
    12361 cgagcacgtc cactattcgg cggccaagtc cggcgtgatc ggccttgtgc gcggccttgc
    12421 tgtcgagctt gcgccggaag gcgtgcgggt gaacggcatt gcgcccggct atatccgcac
    12481 cgcgcaggcc ctttcgaccg accattcgct gggtgcgaag ggattggaag ccgcctccgg
    12541 ctatattccg atgcgccgcg tcggcgagcc ggaagacatc gccgacgtcg cggtcttcct
    12601 tggctccgag caatcgcgat atattaccgg gcaagtgatc accgtcgacg gcggtttgct
    12661 ggtcgggcgc ttctgataaa ggcttctaaa gagggcgccc gccgcccagc acctacattt
    12721 agccacgcag ggagaagatc atggccaaga agatttactg tgcgttcggc accgatatcg
    12781 attccgttgc cggttggatc ggatcctatg gcggcggcga cagcccatcc gatattcagc
    12841 gtggcatctt cgccgccgag gtcggcgtgc cgcgcctgct gaagctattc cggaaatacg
    12901 acctgccgac caccttcttc attccggggc actcgcttga gacctttccc aaggaaatga
    12961 agatgatcgt ggatgccggg catgagatcg gcgcgcatgg ctacctgcac gaaaacccga
    13021 tcgcgatgac ggcgcagcag gaagaggacg tgctggcgaa atcgatcgaa ctgatcgaga
    13081 agcttagcgg gtcggtaccg cgcggctacg tggcgccgtg gtgggaaatg tcggcatcga
    13141 cggcggacct gcttcagaaa tacggcttca gctatgatca cagccaagga taccgcgact
    13201 tccagccgtt ctatgcgcgt gtcggcgacg aatggaatgt catcgactac actaagaagg
    13261 ccaaggactg gatgcacccg ctgaagcacg gcaaggagat cgacctggtc gacatcggcg
    13321 ccaattggta cgtcgatgac ttgccgccga tgatgttcat gaagaaggca cccaacagcc
    13381 atggctttgt caatccacgt catatcgagg agatgtggcg cgaccaattc gactgggtct
    13441 accgcgagat ggactatgcc gtattcgctt tcacgatcca tccagacgtt tcgggccggc
    13501 cgcaggtgct gctgatgctt gagcggatga tcgaatatat cagtggccat acgggcgtcg
    13561 aatgggcgac cttcgacaag atcgccaatg atttccgcgc gcgttatccc ttcgacggcg
    13621 gcaaacggcc tgaggtgatc tgacccgtgt gcagcgcctg cggttttcca tcgctgccgg
    13681 gtcactggac cgacgccggt gcgcgaagcc ccggcagtcg gctcaggttg cgcttcacgc
    13741 gcctgacgat gatcaaccgg ctgctggcgc catacaggct gcgcgcccac gacgatggat
    13801 cgacgcccgg actgcaactc atggcgcccg atggggagcg cgtcctggtg cctgatctgg
    13861 aggcgctgtg ggtacaagcc gagcggctcg ccggtacgcc catcgacccg ctatcgccgc
    13921 gttttctggg cgatgcgtaa aggtgcacgc agcgatggcc gcctgcggct gaccatcatt
    13981 gccgggtttc tcggcgccgg caagagcaca tggctgcgcc accagctgca cgagaatacg
    14041 ttcggttccg tccatgtgtt cgtcaacgag gccgccgaga tgcccgtcga caacctgctg
    14101 ctcggcaagg ctgaccggat ttccgttctg gccggcggtt gcgcctgctg cgagggtcgc
    14161 gcgacacttg tggaggctct gcgcaacacc tgccaggaac aggacggagc cgacgcacaa
    14221 ccgatcgacc gaatattgct ggagaccagc gggctggccg accccggcgc catcaccaag
    14281 gccctctccg aagaccccat cctggcgcgc cgccttgttc ttgacgaaac catagtcctc
    14341 gtcgatgcct tgcatggtct ggcccagctc aatgaagaga gactgggaag acgccaggtg
    14401 gaggctgccg atcggcttat catcaccaag ccgggaagcg tggcggcatc gcagcttgcc
    14461 aggatggcct ctacgctgcg gcagctaaac ccgatggcat tggttgaaag cgccgagcgt
    14521 ggaacttcct tcgcgttgca gacggttccc gacgccgaac cctatccgct tgccgacata
    14581 gccgacgacg ctatgccgat aaagccgtgc cgtctcgata tttccggcga atatggctgg
    14641 gcggctttct cggtctggct ttccgcgctg cttgccgcac gcggcgagca gatcatgcgc
    14701 gtgaaaggcg tggtgaccac cgccgatggc cgccttctgc tgcaatcggt tcgcaagcag
    14761 gtgcaacctc ctgaaatgct tccggaaatg gacgccgaca gttcagttca agggccgcga
    14821 gataacatca tcatattcat cgggcgcggt atagacgatg cgctgctaac ctcggcctgg
    14881 gccagattca cgcaagataa ccccggccaa atgcgccgtt aatgggtgga acagcctgct
    14941 attcccacct ttagcatccg cgattcaccg ccgatccaag cacgccgacg ccggttgtcg
    15001 gcggacgacg ttcttgggcg gcaccacagc tatccaaaac ctttcgaaca aaatcgagcg
    15061 cgcgaacctt gtcctgccag gtcggcactc cggcattccc aatgcgactc atgaacgcca
    15121 gttgcagccg gtcttccagc tggcttggaa gcctacatct cgtcgccgat cttgcgtatc
    15181 ctgtcgacca gttccttgcc gccgaccttg tcgtagaact ggggatagat cgaataagca
    15241 atatcctgga acttcttgat atccgggtgg gtgacggtct tgccttcctt cacccattcg
    15301 gcctcgacct tcttgttgta ggcttcgtcg tccttgcgct cgatgtcgcg gccttccgct
    15361 tcggctttca tgattgcggc g
//
LOCUS       DASYCS010000459         2412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_141488,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000459 DASYCS010000000
VERSION     DASYCS010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141488"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VGM46_14300"
     CDS             <1..189
                     /locus_tag="VGM46_14300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193814.1"
                     /translation="QEQVLDRRSVDEAHAGIPCGRRGPVMRLLARLDALYEENPLAQA
                     TQWILPRSGLAGKARAAE"
     gene            complement(451..1731)
                     /locus_tag="VGM46_14305"
     CDS             complement(451..1731)
                     /locus_tag="VGM46_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007198964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY4193815.1"
                     /translation="MSFDIIVAGGTLPDGRVADIGIRGETIAAIEPKLDGEAGTVVDA
                     SGDLVSAPFVDPHFHMDATLSYGLPRINASGTLLEGIALWGELKPLLTHEAVRDRALA
                     YCDWAVSMGLLAIRTHVDVCDDRLLAVEALLDVKEKVAPYIDLQLVAFPQDGFYRSPT
                     ARDNTIRALDKGVEIVGGIPHFERTMADGARSVTELCEIAAKRGLMVDMHCDESDDPH
                     SRHVEQLAYETQRLGLQGRVAGSHLTSMHSMDNYYVSKLLPLIAEADLSVIPNPLINM
                     MLQGRHDTYPKRRGLTRVPEMLKLGIRVGWGQDCVLDPWYSLGTADMLDVAFMGLHAA
                     QMSSPADMARCFDMVTNVNAKIMGLDHLGLEVGKRASLVVLDAGNAIEAVRLRPDRLH
                     VISRGKIVASREKRDTKLSIGGRPGSINRRHKMQ"
     gene            complement(2058..>2412)
                     /locus_tag="VGM46_14310"
     CDS             complement(2058..>2412)
                     /locus_tag="VGM46_14310"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006697627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEY4193816.1"
                     /translation="PTSYLKSKGLGKVCALLTDGRFSGGTSGLSIGHVSPEAEEGGLI
                     GLVREGDTIEIDIPNRTINLAVDDAELARRRAEQDKLGWKPAEVRKRKVTQALRAYAA
                     FATSADKGAVRYVPE"
BASE COUNT          443 a          747 c          789 g          433 t
ORIGIN      
        1 caggagcagg ttcttgatcg caggtctgtg gacgaggctc atgccggcat tccttgtggc
       61 cgaagggggc cggtaatgcg gctgttagcc cggcttgatg cgctttacga ggaaaatccg
      121 ttggcgcagg caacccaatg gattttgcct agatctggcc tggccggcaa ggcgcgcgcg
      181 gctgaatgac ggcccgacga cagatgacgc gcggcgtagg cagcggggcg aaattgcgac
      241 cttggcgctg tgcggcgcgc cggtcgaggc cgatgcgctg gagactgccg gcgcaagcat
      301 ctttttaatg gagaccgggc gagcggcact tggccagacc gacagcccgc tggttccgcg
      361 ccgtcatcca tccgctcaag agcaccgcca ccgcgacgcg aataatggcg aggcatggcg
      421 gtaggcgaca ggcagccccc gagtttgccg ctattgcatc ttgtggcggc ggttgatcga
      481 gccgggccgc ccgccaatcg acaatttcgt gtcgcgcttt tctcgcgagg cgacaatttt
      541 gccgcgcgaa atcacatgca gccggtccgg acgcaggcga acggcctcga tggcgttgcc
      601 ggcatcgagc acgacaagac tggcgcgttt gccgacttcc aggccgagat ggtcgaggcc
      661 catgatcttg gcgttgacgt tcgtcaccat gtcgaagcag cgcgccatgt cggccgggct
      721 ggacatttgc gcggcgtgca ggcccataaa ggcaacgtcg agcatgtcgg cggtgcccag
      781 cgaataccat gggtcgagaa cgcaatcctg gccccagccg acacggatgc cgagcttcag
      841 catttccggc acgcgggtca ggccgcgccg cttcggatag gtgtcgtggc ggccttgcag
      901 catcatgttg atgagcggat tggggatgac cgacaggtcg gcctcggcga taagcggcag
      961 cagcttcgag acatagtagt tgtccatcga gtgcatcgag gtcaggtgcg agccggccac
     1021 cctgccctgc aagcccaggc gctgcgtctc ataggccagt tgctcgacat ggcgcgaatg
     1081 cggatcgtcg ctctcgtcgc aatgcatgtc gaccatcagg ccgcgcttgg ccgcgatctc
     1141 gcacagttcg gtgaccgaac gggcgccatc ggccatggtg cgctcgaaat gcggaatgcc
     1201 gccgacgatc tcgacgccct tatccaaagc gcggatcgta ttgtcgcggg ccgtgggcga
     1261 gcggtaaaaa ccgtcctgcg gaaaggccac cagttgcagg tcgatatagg gcgcgacctt
     1321 ttccttcacg tcgagcagcg cctcgacggc cagcagcctg tcgtcgcaga catcgacatg
     1381 ggtgcgaatg gcgagcaggc ccatcgaaac ggcccagtcg caataggcca gcgcacggtc
     1441 gcgcaccgcc tcatgcgtca aaagcggctt caactcgccc catagcgcaa tgccttccag
     1501 cagcgtgccc gatgcgttga tgcgcggcag gccataagaa agcgtggcat ccatgtggaa
     1561 atgcggatcg acgaagggag cggaaaccag atcgccggaa gcgtcgacga ccgtgcctgc
     1621 ttcgccatcc agcttcggct cgatggccgc gattgtttcg ccacgaatgc cgatatcggc
     1681 cacccggccg tccggcaagg tgccgcccgc aacgatgatg tcaaagctca ttttttagtc
     1741 cttggtttag accgatgttt tcgaacacag cgccatcggg gaaacggtgt gccaattccg
     1801 ccgctgactg tctaccgcct tgatttgcgg gcggtcacgg cgtggattcc cggcactctc
     1861 cgctccctgc gatcgctcac gaggccggga atgacgggtg tattcaaaca gctccggtcg
     1921 ccatgcaggg aagcggaaat taaaagaact gtcattcccg gcctcttgag ggagcggaac
     1981 gaccggagag tgtcgggaat tcatgccccc ggcactctca tggaaatcgc tgccgcaccg
     2041 gccgctggaa agacagccta ctccggcacg tagcgcaccg cgcccttgtc ggcgctggtg
     2101 gcgaaggcgg cgtaggcgcg cagcgcctgc gtcaccttgc gcttgcgcac ttcggccggc
     2161 ttccagccaa gcttgtcctg ctcggcgcgg cggcgggcaa gttcggcatc gtccacggcg
     2221 aggttgatgg tgcggttggg aatgtcgatc tcgatggtgt cgccttcgcg caccagcccg
     2281 atcagcccgc cctcctcggc ttccggcgag acatggccga tggacaggcc ggaggtgccg
     2341 ccggaaaaac ggccatcggt cagcagcgcg cagactttgc ccaggccttt cgacttcaga
     2401 tagctggtcg ga
//
LOCUS       DASYCS010000460         2485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_4054600,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000460 DASYCS010000000
VERSION     DASYCS010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4054600"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1446)
                     /locus_tag="VGM46_14315"
     CDS             complement(<1..1446)
                     /locus_tag="VGM46_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018448873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEY4193817.1"
                     /translation="MALLASGSINPGMLQPAPAEGPPLRGAARETFAAGADLAAASHA
                     PAAAFASAEAGKRPATALIAALPARLARLATQVLLISGDVAAYSIACLLLVPFHPGAI
                     EDGALVRAFILVAMAALVFYASEALYPGYRLHGHEHLRRRAKATFKVAALAVFGSMLL
                     PGGWPLALPIAGFLGIALIVQPLTHRTARRLCRWLGLWGERAAIIAGPNRVPSLIEHF
                     NRHWQYGLRPEPFLRDTAPSGNGPRIALIAGDTAPVLDELSALRREFAEVVLLADTPH
                     LKVAGLRPADIGGQVGLHLAGRDALPAPYLLRRLVDLMIAVPAAIVSAPLLALAGAAI
                     YAIDPGPVFFWQPREGLAGKTVRVLKLRTMYRDAEQRLEALLAENPAVQAEWSAHFKL
                     KHDPRILPVVGQLLRSSSFDELPQLFNVIIGEMGIVGPRPFPEYHLLAMNAEFRHKRR
                     SVTPGLTGLWQISERSNADLELQRQLDEFYID"
     gene            complement(1619..2434)
                     /locus_tag="VGM46_14320"
                     /pseudo
     CDS             complement(1619..2434)
                     /locus_tag="VGM46_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018483898.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
BASE COUNT          455 a          808 c          805 g          417 t
ORIGIN      
        1 gtcgatgtag aattcgtcga gctggcgctg tagttcgaga tcggcgttgc tgcgctcgga
       61 tatttgccaa agcccggtca agccgggcgt taccgagcgc cttttgtggc ggaactcggc
      121 attcatcgcc agaagatggt attctgggaa cgggcgcggt ccgacgatgc ccatttcgcc
      181 gatgatgacg ttgaacagtt gcggcagctc gtcgaagcta ctggagcgca gcaactgccc
      241 gaccaccggc aggatgcgcg gatcgtgttt gagcttgaag tgtgcggacc attcggcctg
      301 cacggcgggg ttctcggcaa gcaatgcctc aagccgctgt tcggcatcgc ggtacatggt
      361 gcgcagtttc agcacgcgca ccgtcttgcc ggccaggccc tcgcgcggct gccagaagaa
      421 gacggggccg gggtcgatcg cgtagatcgc cgcgcccgcc agcgctagca atggcgccga
      481 cacgatggcg gccggcacgg cgatcatcag gtctaccagc cgccgtagaa gatatggcgc
      541 aggcagggca tcgcggccgg ccagatgcag cccgacctgg ccgccaatgt cggccggccg
      601 caagcctgcc accttcaaat gcggcgtatc ggcaagcagc accacctcgg cgaattcccg
      661 gcgcaacgcg ctcaattcgt caagcacggg cgcggtgtcg ccggcgatga gggcgatgcg
      721 cgggccattg ccggaaggcg cagtgtcgcg caaaaacggt tccggccgca acccatattg
      781 ccagtgacgg ttgaaatgct cgatcagcga gggcacccgg ttcgggccgg caatgattgc
      841 cgcccgttcg ccccacaggc cgagccatct gcacagccgc cgcgcagtgc gatgcgtcag
      901 cggctggacg atcagcgcga tgccaaggaa gcccgcgatg ggcaaagcga gcggccagcc
      961 tccgggaagc agcatcgagc cgaagacggc aagcgctgcc accttgaagg tagccttcgc
     1021 gcggcggcgc agatgctcat ggccgtgaag ccggtatccg ggatagagcg cttcggatgc
     1081 gtagaacacc agcgctgcca tcgcaacgag tatgaaggcc cgcacaaggg cgccatcctc
     1141 gatagcaccg ggatggaacg gaacgagaag aaggcaggcg atggaatagg ccgcgacatc
     1201 gccggaaatc agcagcactt gtgtggcaag ccgcgccagc ctggcaggta aggctgcgat
     1261 gagggctgtc gccgggcgct ttcccgcctc ggcgctcgca aacgcggctg ccggcgcatg
     1321 cgatgcggcc gccaagtcgg cgccagccgc aaaggtttcc cgcgcagcgc cgcgtagtgg
     1381 cggcccctcg gcgggggccg gctgtagcat gccgggattt atgcttcctg aggcgagtaa
     1441 tgccattcca gttcactctt gcggtttgtt cggtacgacg atttcactcg tcaagcagtc
     1501 gtgtcgccag cgcccgctgc acccgcagcg ctccatccaa tccttgcgcc atgccggcgg
     1561 tttcaagcat ggccgcctca aagccatgcc tgccgccggt tgccgttccg cgctgcttgt
     1621 cgatggggaa ttgccgcaac cccaatctct gcaacgcggt aagccagagg aaacccggca
     1681 ccaccaccgc gtagcgccgc cacagccgac gcggttcgca agcaagccgg aaggcccact
     1741 cgaaaccgct tcgctgaatt gcttttggag cctgccgctt caggccggca tggaaatcga
     1801 aagccgcccc gacgccgatg agcacgggcg catcgagacg gccgcgcatc tgcgccatcc
     1861 acagttcctg ctttggcgtg ccaaggccga cccacaccac atcggctgcc gaccggttga
     1921 tccgcgcagc gatgtcggtt tcttcctgtg tcgacagcgc atgaaaaggc ggcgcataag
     1981 cgccgacgat ctgcgcgcct ggaaagcgcg ccagcagttt ttcctgtagc cggctcaacg
     2041 tatcttccgt tgcgccatag aggaaatggc gaaagccttt cgccacgccg gtctcgaaca
     2101 gcgaaagcat caggtccggc ccgtagaccc ggccggcatc ctcgtggccg gcgcgcttga
     2161 gcgcccagac gagaggcatg ccgtcgggcg tcaccaggaa ggcgcggtta tgcgccgagc
     2221 gcagtttcgc gtcgctctgg cagcggatga cgccatgcgc atcccggatg cagacatatt
     2281 cgcggcggcc ttccgcgatc gcggtttcga tccgcgcggc ggcatccttc agattgacgg
     2341 ccgaaacggc gacgccgagc acgttgaccc aggccggggc ctgttcagga cgttcgcttg
     2401 cggcgaaatg ctcgttttgc cgtacgcttt tcattttatg ccttctgttg ccgttcaccg
     2461 gcctttgccc cagccgggaa ccagt
//
LOCUS       DASYCS010000461         4378 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2460405,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000461 DASYCS010000000
VERSION     DASYCS010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2460405"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /gene="flgA"
                     /locus_tag="VGM46_14325"
     CDS             <1..348
                     /gene="flgA"
                     /locus_tag="VGM46_14325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HEY4193818.1"
                     /translation="EIVALGALKEVTLVPGRKKPDAVATLIGELDGKIAKRTLLPGHY
                     IPVNALRDPWVVEQGAPVQVIFASGPLTISAAAVTLQPGSTGDLVKVRNLDSGKIFSG
                     TVMADGTIRVGAS"
     gene            345..1589
                     /locus_tag="VGM46_14330"
     CDS             345..1589
                     /locus_tag="VGM46_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006204087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HEY4193819.1"
                     /translation="MKRGLVLSLAMAVLCLHPALADGLTPKAKRELAARNGPAFNDPD
                     YDPKATARMFQVSSGPSSLPPGQVASRIKDIAQLQSARDNQLVGYGLVIGLQGSGDSL
                     RNSPFTEQSIRAMLENLGIATLDGAARAKNVAAVIVTANMPPFVQSGARIDIDVSSMG
                     DATSLSGGTLVMTPLKAADGQIYAVGQGSVIVSGFNAQGQAEQLTQGVPTSGRVPNGA
                     IVERSVDAEFGDQSVLTLQLRNPDFSTAVRIADAINDYTGQRFGVRVAAERDARTVSI
                     KKPKGISAARFYAEIENLIVESDTPARVVIDERTGTIVIGNEVKVSRVAISHGTLTVR
                     ITETPTVVQPEPFSKGETAIEPNTAIDVTQPDARVAVLDGPNLETLVSGLNRLGVKPD
                     GIIAILQGIKSAGALQADLVVQ"
     gene            1601..2170
                     /locus_tag="VGM46_14335"
     CDS             1601..2170
                     /locus_tag="VGM46_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotE family protein"
                     /protein_id="HEY4193820.1"
                     /translation="MTPLFPRGARRLALLAAVASMAVSTMTHAETVRQVLPGAPPATA
                     PQEMARVAAPAAGDASEVERFCSNIADAARDRRYALQAAEMKQLQADLDARMTALDEK
                     KAEYETWLKRREVFLARAQDGIVKIYAGMKPDAAAERLAMVNVDLAAAILMKLDSRKA
                     SVILNEMDDKAAAALTSLMASAARRVDPS"
     gene            2167..2871
                     /gene="flgH"
                     /locus_tag="VGM46_14340"
     CDS             2167..2871
                     /gene="flgH"
                     /locus_tag="VGM46_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HEY4193821.1"
                     /translation="MIRKTLFLVAAISLSGCATNFKEVGKEPALSPVGSGIEGGATSV
                     YRYPETPAAPVRKFSLWDDRQSRLFTDPRALREGDILTVKIKINDRAKFKNQNDRSRT
                     ASRSLGFDADLGWDSYKTSGKGSGSLDSSTKTNADGATERSESIELNVAAVVTEVLPN
                     GNLMIKGSQEVRVNAELRVLTIAGIVRPSDIGAENTISYERIAEARIAYGGRGRISEV
                     QQPAYGQQVLDQVLPF"
     gene            2891..3397
                     /locus_tag="VGM46_14345"
     CDS             2891..3397
                     /locus_tag="VGM46_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HEY4193822.1"
                     /translation="MAAVEDIPASKGPSLVIQAALIVGMTVAALGIGWFAGSYLKSSQ
                     APAKVKPELAKHEAKAEEKGKDEKSHDPDAGPLLVELAPITTNLASPATVWVRLDASL
                     VLDAPQPPETIEAVHQDLLALIRTIKMHQVEGGSGYQHLKADLQERASIRSGGHVKDV
                     LIRTLVFE"
     gene            3394..4125
                     /gene="fliP"
                     /locus_tag="VGM46_14350"
     CDS             3394..4125
                     /gene="fliP"
                     /locus_tag="VGM46_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008599004.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HEY4193823.1"
                     /translation="MRILPASIAIVLATISGASAQQIDLGSIAQADGATVGTIIQMFG
                     LLTVLSVAPGLLIMVTSFTRFIIAFSILRAGIGLQTTPANLILISLSLFMTFYVMAPT
                     FDQAWNNGVKPLMDNQITQQVALEKISDPFRTFMLHNVRDKDFDLFADLARERGQVVA
                     RDTVDLRILVPAFMISEIRRGFEIGFLIVMPFLVIDLIVATITTAMGMMMLPPTVVSL
                     PFKIMFFVLIDGWNLLVGSLVRSFN"
BASE COUNT          836 a         1452 c         1305 g          785 t
ORIGIN      
        1 gaaatcgtcg cccttggcgc gctgaaggaa gtaacgctgg ttccgggaag gaaaaaaccg
       61 gatgccgttg ccacccttat tggcgagctt gacgggaaaa tcgccaaacg gaccctgctg
      121 cccggccatt atattcccgt caacgccctt cgcgatccgt gggtggtcga gcagggcgcg
      181 ccggttcagg tcatattcgc gtccggcccg ctgaccattt ccgccgccgc cgtaacgctt
      241 cagcccggct cgacgggcga tctcgtcaag gtccgcaacc tcgacagcgg caagatattc
      301 tccggcacgg tcatggcgga cggcaccatc cgggtcggcg cgtcatgaag cgtggcctcg
      361 tcctttcttt ggcaatggcg gtgctttgcc tgcaccccgc actggctgac ggcctgacgc
      421 cgaaggccaa acgcgagctt gccgccagga acggaccggc cttcaacgat cccgattacg
      481 atccgaaggc gaccgcccgc atgttccagg tatcgtcggg tccaagctcg ctgccgccgg
      541 gccaggttgc gtcccgcatc aaggacatcg cccagctgca aagcgcgcgc gacaaccagc
      601 ttgtcggcta cggtctggtc atcggcttgc agggttcggg cgattcgctg cgcaattcgc
      661 ccttcaccga acaatcgatc cgagccatgc tggaaaatct cggcatcgcg acgctggatg
      721 gcgcggcgcg cgccaagaac gtcgccgccg ttatcgtcac cgccaacatg ccgcccttcg
      781 tccagtcggg cgcgcgcatc gatatcgacg tttcctcgat gggcgacgcc acctcgctct
      841 ccggcggcac gctggtgatg acgccgctga aagcggcgga tgggcagatt tatgccgtcg
      901 gccaggggtc ggtcatcgtt tcgggcttca acgcgcaggg gcaggccgaa caactgacgc
      961 agggcgtgcc gacctccggc cgcgtgccga acggcgccat cgtcgagcgc tccgtcgacg
     1021 ccgagttcgg cgaccagtcg gtgctgacat tgcagctgcg caacccggat ttctccaccg
     1081 ccgtgcgcat cgccgacgcg atcaacgact ataccggcca gcgtttcggc gtgcgtgtcg
     1141 ccgccgagcg tgatgcaagg acggtttcga tcaagaagcc gaagggcatc tcggcggcgc
     1201 gcttctatgc cgagatcgag aacctgatcg tcgaatccga tacgccggcg cgcgtcgtca
     1261 tcgacgagcg caccggcacc atcgtcatcg gcaacgaagt caaggtttcg cgcgtcgcca
     1321 tcagccacgg cacgctgaca gtccgcatca ccgaaacgcc gacggtggtt cagcccgaac
     1381 cgttctccaa gggtgaaacg gcgatagaac cgaacaccgc gatcgacgtc acccagcccg
     1441 atgcgcgcgt cgccgtgctt gacggcccca accttgaaac gctggtttcc ggcctgaacc
     1501 gcctcggcgt caagcccgac ggcatcatcg ccatcttgca gggcatcaaa tcggccggtg
     1561 cattgcaagc cgacctggtc gtgcaatgag gaacctgccg atgaccccgc ttttccccag
     1621 gggcgcccgc cgccttgccc tgctcgctgc ggtcgcgtcg atggcggtat ccaccatgac
     1681 ccatgccgag acggtcaggc aggtgcttcc cggcgcgccg cccgcgaccg cgccgcagga
     1741 gatggccagg gttgccgcac ccgccgccgg cgacgcaagc gaggtcgagc gcttctgctc
     1801 caacatcgcc gacgccgccc gcgaccgtcg ctatgccttg caggccgccg aaatgaaaca
     1861 gttgcaggcc gatctggacg cccgcatgac agcgctcgac gaaaagaagg ccgagtacga
     1921 gacatggctg aagcgccgcg aggtcttcct cgcccgcgcc caggacggca tcgtcaagat
     1981 atatgccggc atgaagccgg acgcggcggc tgaacggctg gctatggtca atgtcgatct
     2041 tgcagccgcc atcctgatga aactggattc gcgcaaggca agcgtcatcc tcaacgaaat
     2101 ggacgacaag gcagcagcgg cattgaccag cctgatggcc agcgccgccc gcagggtgga
     2161 cccgtcatga tccgcaagac gctttttctc gtggcggcga taagcctttc gggctgcgcc
     2221 accaatttca aggaagtcgg caaggaaccg gcgctttcgc cggtcgggtc cggcatcgaa
     2281 ggcggcgcga cctccgtcta tcgttacccg gagacgccgg ccgccccggt ccgcaaattc
     2341 tccctgtggg acgaccgcca aagccgcctg ttcaccgacc ctcgcgcatt gcgtgaaggc
     2401 gacatcctga ccgtcaagat caagatcaac gatcgcgcca agttcaaaaa ccagaacgac
     2461 aggagcagga ccgcctcgcg ctcgctcggg tttgacgccg accttggctg ggattcctac
     2521 aagacgagtg gcaagggcag cggcagcctg gattccagca ccaaaaccaa tgccgacggc
     2581 gctaccgagc gctcggaatc gatcgaattg aacgtcgccg ccgtcgtcac cgaggtcctg
     2641 cccaacggca atctgatgat caaggggtcg caggaagtgc gcgtcaacgc cgagttgagg
     2701 gtgctcacca tcgccggcat cgtgcgcccg tccgacatcg gcgcggagaa taccatttcc
     2761 tacgagcgca tcgccgaggc ccgcatcgcc tatggcgggc gcggacgcat cagcgaggtt
     2821 cagcagccgg cctatggcca gcaggtgctg gaccaggtgc ttcctttcta agatggcgaa
     2881 aggcaaggcc gtggcggccg tagaagatat cccggcttcg aaagggccgt ccctcgtcat
     2941 ccaggccgcc ctgattgtgg gcatgacggt cgccgcgctc gggatcggct ggtttgccgg
     3001 ttcctacctc aaaagctcgc aggcgcctgc gaaggtcaag cccgaattgg cgaaacacga
     3061 agccaaggcc gaggaaaagg gcaaggacga aaagagccac gatccggacg ccggcccgct
     3121 gctggtcgaa ctcgcgccga tcaccaccaa cctcgcttcg ccggctaccg tctgggttcg
     3181 cctcgacgcc tcgctggtgc tcgacgctcc gcagccgccg gaaacgatcg aggcggttca
     3241 tcaggatttg ctggcgttga ttagaacgat caagatgcac caggtcgaag gcggcagcgg
     3301 ctaccagcac ctcaaggccg atctccagga gcgcgcctcc atccgcagcg gcggccacgt
     3361 caaggacgtg ctcataagga ccctcgtatt cgaatgagaa tacttcccgc cagcatagcc
     3421 atcgtgcttg ccaccatttc cggcgcatcc gcccagcaga tagaccttgg cagcatcgcc
     3481 caggcggacg gcgcaacggt cggcaccatc atccagatgt tcggcctgct caccgtcttg
     3541 tcggtggcgc ccggcctgct catcatggtt acaagcttca cgcgtttcat catcgccttt
     3601 tcgatcctgc gcgccggcat cggcctgcaa acgacgccgg cgaacctcat cctcatatcg
     3661 ctgtcgctgt tcatgacctt ctacgtcatg gcccccacct tcgatcaggc atggaacaat
     3721 ggcgtcaagc cgttgatgga caaccagatc acgcagcagg tggcgctgga aaagatatcc
     3781 gacccgttcc gcaccttcat gctgcacaat gtgcgcgaca aggatttcga cctgttcgcc
     3841 gaccttgcgc gtgaacgcgg ccaggtggtc gcgcgcgaca ctgtcgacct gcgcatcctg
     3901 gttccggcct tcatgatttc cgaaatcagg cgcggtttcg aaatcggctt cctgatcgtc
     3961 atgccgtttc tcgtcatcga cctcatcgtc gccaccatca ccacggcgat gggcatgatg
     4021 atgctgccgc cgaccgtggt gtcgctgccg ttcaagatca tgttcttcgt gctgatcgac
     4081 ggctggaacc tgttggtcgg cagtctggtg cgctcgttca actgaccgcc gacctggcat
     4141 ttcacccgcc gttcaccacc cgttaaccat gatttttatg cctttcgcag gaactgtctg
     4201 ccatgttgcg ggctgatggc gggtgattgg gtttaaacga tcattggcat gatgccgtac
     4261 cgtcataccg gacaagccgg tatgtcaaag taatgcgtgt tatcgcgtaa aggtacgagt
     4321 acccatgtcc agcatcatga ccaacgcttc ggcgttgacg gctcttcaga gcctgaat
//
LOCUS       DASYCS010000462         5549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2896449,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000462 DASYCS010000000
VERSION     DASYCS010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 5549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5549
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2896449"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="VGM46_14355"
     CDS             complement(<1..860)
                     /locus_tag="VGM46_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018430070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4193824.1"
                     /translation="MTMTPSATTSAMTENILQLEHLSMKFGGLVAIGDLSFAAKRGEI
                     TALIGPNGAGKTTVFNCITGFYKPTEGMIRFTGKAGNEFLLERMADFQITAKAKVART
                     FQNIRLFSGMTVLENLLVAQHNKLMKASGYTVLGLLGIGGYGKASAESVELAKHWLDK
                     ADLTERADDPAGDLPYGAQRRLEIARAMCTGPELLCLDEPAAGLNPRESLALNALLID
                     IKDTTGTSILLIEHDMSVVMQISDHVVVLEYGRKIADGSPAAVKTDPRVVAAYLGVDD
                     EEVEDVLGEVG"
     gene            complement(860..2455)
                     /gene="livM"
                     /locus_tag="VGM46_14360"
     CDS             complement(860..2455)
                     /gene="livM"
                     /locus_tag="VGM46_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivM"
                     /protein_id="HEY4193825.1"
                     /translation="MANTVSTEQATRAGVTSRALREAFFAGLMAFGCFVLLIGLKTDQ
                     NIRNELVLVQRWGLLAIVVALTMVGRFCFIAYLQPFLARQKIADVATGLLPASAATRF
                     FRLPYFIVALVVIAVLIVAAGSLDGMLGAELAGYARFLRALAIIYALASVLFYFRAFI
                     LGHFSALGVSVLALYPVFVVLVLALLSGSMAAGFQGSLKWVDNFGIQILIYVMLAWGL
                     NIVVGLAGLLDLGYVAFYAVGAYAYALLGTQYGLSFWILLPAAGIMAAFWGVILGFPV
                     LRLRGDYLAIVTLAFGEIIRMVLINWREVTNGSAGISGIPKVSFFGLFTFNVSDPNYI
                     SKVLHIAQSGAYYKIFLYYLILLLCLLTAFVTIRLRRMPVGRAWEALREDEIACRSLG
                     INTTTTKLTAFATGAMFGGFAGAFFAARQGFVSPESFVFLESAIILAIVVLGGMGSLV
                     GIAIAAVVMIGGTEILRELDFLKVVFGPDFTPELYRMLLFGLAMVVVMVWKPRGFVGS
                     REPTAFLKERKAVSASFTKEGHG"
     gene            complement(2460..3362)
                     /locus_tag="VGM46_14365"
     CDS             complement(2460..3362)
                     /locus_tag="VGM46_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006471258.1"
                     /note="LivHMGF is the membrane component of the LIV-I/LS
                     branched-chain amino acid transporter; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease LivH"
                     /protein_id="HEY4193826.1"
                     /translation="MQYFIQQLINGLTLGSIYGLIAIGYTMVYGIVGMINFAHGDIFM
                     VGAFVALIVFLVLGALFYTVPVVVALLIMMIVSMLLVSLYNWVIEKIAYRPLRGSFRL
                     APLITAIGMSIALSNFVQVTQGPRNKPIPPMISQVYNIYGVSISLKQIVIVLVTAILL
                     VVFSYIVNKTSLGRAQRACEQDQKMAALLGIDVDRTISITFIIGAALAGVAGTLFLMY
                     YGVVVFSDGFVPGVKAFTAAVLGGIGSLPGAVIGGLLIGFIESMWSAYFSIDYKDVAA
                     FSILAIVLIFLPSGILGRPEVEKV"
     gene            complement(3696..4697)
                     /locus_tag="VGM46_14370"
     CDS             complement(3696..4697)
                     /locus_tag="VGM46_14370"
                     /EC_number="5.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyproline epimerase"
                     /protein_id="HEY4193827.1"
                     /translation="MAKKSFFCIDGHTCGNPVRLVAGGGPLLEGTTMMERRAHFLAEY
                     DWVRTGLMFEPRGHDVMSGSILYPPTREDCDIAILFIETSGCLPMCGHGTIGTVTMAI
                     EQGLIKPKTPGMLRLDTPAGLVTAEYKQVGEYVEEVRITNVPSFLYAEGLTVECPQLG
                     EITVDVAYGGNFYAIVDPQKNYRDMADYSAGDLIAWSPVVRQRLNEKYSFAHPENPGI
                     NRLSHMLWTGAPTVEGADARNAVFYGDKAIDRSPCGTGTSARMAQLHAKGRLKAGDTF
                     VHESIIGSLFHGKVEKEVSVAGKTAIVPSIGGWARMTGLNTIFIDDRDPFAHGFIVK"
     gene            complement(4709..>5549)
                     /locus_tag="VGM46_14375"
     CDS             complement(4709..>5549)
                     /locus_tag="VGM46_14375"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY4193828.1"
                     /translation="REDGSLELYESEPEFRASLSGWAARDRFGIGYRHVEGRELADLQ
                     PGLSPRFIKGTFVPGWKTVADPKLLGKAIWAYAETKGARFERARIERIDAVEAGATLT
                     LAGGAVRKAKLVVLAAGAWSHLLARNLGDRIPLETERGYNTTLPASAYEVKRQLIFSG
                     HGFVITPLSTGLRIGGAVELGGIERAPNYARSRALLAKAQKFLPGLDASGGREWMGFR
                     PSLPDSRPVIGRAPSHPCVLYAFGHGHLGLTQAAATGRLIRDLVSGQAEALDLSPFSP
                     QRF"
BASE COUNT         1152 a         1754 c         1718 g          925 t
ORIGIN      
        1 ccgacttcgc caagcacgtc ctcgacttcc tcgtcgtcga cacccagata ggccgcgacg
       61 acgcgcgggt cggtcttcac cgccgccggg ctgccatcgg cgatcttgcg cccatattcc
      121 agcaccacga catggtcgga gatttgcatc accaccgaca tgtcgtgctc gatcagcagg
      181 atcgacgtgc ccgtggtatc cttgatgtct atcagcagcg cattgagcgc cagggactcg
      241 cgcgggttga ggcccgcagc cggctcgtcc aggcacagaa gctccggccc cgtgcacatg
      301 gcgcgtgcga tttccagccg ccgctgcgca ccatagggca gatcgcccgc cggatcgtcg
      361 gcacgctcgg ttaggtcggc cttgtccagc caatgcttgg caagctcgac cgattcggcc
      421 gaggccttgc cgtagccgcc aatgccaagc aggccgagca cggtgtagcc cgatgccttc
      481 atcagcttgt tgtgttgcgc gaccaacagg ttctcgagca ccgtcatgcc ggaaaacagg
      541 cggatattct gaaacgtgcg cgcaaccttg gccttggcgg tgatctggaa gtcggccatg
      601 cgctccagca ggaactcgtt gcctgccttg ccggtaaacc ggatcatgcc ttcggtcggc
      661 ttgtagaagc cagtgatgca gttgaacacc gtcgtcttgc cggcgccgtt cgggccgatg
      721 agcgcggtga tctcgccgcg cttggctgcg aaggaaaggt cgccgatggc gaccagcccg
      781 ccgaacttca tcgacagatg ttcgagttgg aggatgttct cggtcatggc gctcgtcgtc
      841 gcggatgggg tcatggtcat cagccgtgtc cttccttggt gaaggaggcg gatacggcct
      901 tgcgttcttt caggaacgcc gtcggttcac ggctgcccac gaaaccgcgc ggcttccaca
      961 ccatcaccac gaccatcgcc aggccaaaca gcagcatgcg gtaaagttct ggcgtgaagt
     1021 ccggcccgaa caccactttc aggaagtcga gttcacgcag gatttcggtg ccgccgatca
     1081 tcactaccgc cgcaatcgcg atgccgacca gcgaacccat gccgccaagg acgacgatgg
     1141 cgagaatgat cgccgattcg aggaagacga aggattcggg gctgacgaag ccctggcgcg
     1201 ccgcgaagaa cgcgccggcg aagccgccga acatcgcgcc ggtggcaaac gccgtcagct
     1261 tggtcgtggt ggtgttgatg ccgagcgagc ggcaggcgat ctcgtcctcg cgcagcgctt
     1321 cccaggcgcg tccgaccggc atccggcgca gccggatggt gacgaacgcg gtcaagaggc
     1381 acaggagcag gatcaaataa tagaggaaaa tcttgtagta ggcgcccgac tgcgcgatgt
     1441 gcagcacctt ggagatgtag ttcgggtccg acacgttgaa agtgaacaag ccgaagaacg
     1501 acactttcgg aatgcccgaa atacccgccg aaccgttggt gacctcgcgc cagttgatca
     1561 gcaccatgcg aatgatttca ccgaaggcga gcgtcacgat ggcgaggtaa tcgccgcgca
     1621 ggcgcagcac cggaaagccc aggatcacgc cccagaacgc cgccatgatg ccggcggcgg
     1681 gcagcaatat ccagaacgac aggccatatt gggtgccgag cagcgcatag gcataggcgc
     1741 caaccgcgta gaaggcgacg tagccgaggt cgagcaggcc ggcgaggccg acgacgatgt
     1801 tcagccccca ggccagcatc acgtagatca ggatctggat gccgaaatta tcgacccatt
     1861 tcagcgaccc ctgaaaaccg gccgccatcg atcccgaaag cagcgccagc accagcacca
     1921 cgaacaccgg gtagagcgcc agtacgctga cgcccagcgc cgagaaatga ccgaggatga
     1981 aggcgcggaa ataaaacagc accgacgcca gcgcatagat gatggcgagg gcgcgtagga
     2041 agcgcgcata gccggccaac tcggcgccca gcatgccatc cagcgagccc gccgccacga
     2101 tcagcacggc aatgacgacc agcgccacga taaagtaggg cagccggaag aaccgcgtcg
     2161 ccgcgctggc gggcaaaagc ccggtggcga catcggcgat tttctgccgc gccaggaacg
     2221 gctgcaaata ggcgatgaag cagaaccgcc cgaccatggt gagcgcgacg acgatggcca
     2281 gaaggcccca gcgctgcacc agcaccagtt cgttgcggat gttctggtcg gtcttcaacc
     2341 cgatcagcag gacaaaacag ccgaaagcca tcaggccggc aaagaacgct tcgcgaaggg
     2401 cgcgcgaggt aacgccggcg cgggtagctt gctccgtgga aaccgtattg gccatggtgt
     2461 cagaccttct cgacttctgg ccggcccaat atgccggaag gcaggaaaat cagcacgatc
     2521 gccaggatgg aaaaggccgc cacgtccttg tagtcgatcg agaaataggc cgaccacatg
     2581 ctctcgatga acccgatcag cagaccgccg ataaccgcgc ccggaagcga gccgataccg
     2641 cccagcaccg ccgccgtgaa cgccttgacg cccggcacga agccgtcgga gaacaccacc
     2701 acgccgtaat acatcaggaa cagcgtgccg gcgacgccgg caagcgcggc gccgatgatg
     2761 aaggtgatcg aaatggtgcg gtcgacatcg atgccgagca gcgccgccat cttctggtcc
     2821 tgctcgcagg cgcgctgggc gcggccgagc gacgtcttgt tgacgatgta ggaaaacacc
     2881 accagcagga tcgcggtgac gagcacgatg acgatctgct tcagcgagat gctgacgccg
     2941 tagatgttgt agacctgact gatcatcggc gggatcggct tgttgcgcgg accctgcgtg
     3001 acctgcacga aattggagag cgcgatcgac atgccgatgg cagtgatcag cggcgccagg
     3061 cgaaacgagc cgcgcagcgg ccggtaggcg atcttctcga tcacccagtt gtaaaggctg
     3121 accagcagca tcgaaacgat catcatgatc agcagcgcga cgaccaccgg caccgtatag
     3181 aacagcgcgc ccaggacgag aaagacgatg agcgcgacga aagcgccgac catgaagatg
     3241 tcgccatgcg cgaaattgat catgccgacg atgccgtaga ccatcgtata gccgatagcg
     3301 atcaggccat agatcgatcc cagcgtcagc ccgttgataa gctgctggat gaaatactgc
     3361 atgttggtat ggctcccctg gaacgtcgcc catgcagacg cgttcctttt cgtatttccc
     3421 ctagtcgtaa agtttcaggc ccgcaatgca atgcccttct tttgtcgaag gaacgtgtta
     3481 tcggcgctgc cgattcccat ccgtcttcac gcatccggcc tgtggcccac ggcggaccat
     3541 acgcttggta taacttaatg tctttgctga ttcgagccta gtttgcacct gttttcgaag
     3601 aaatcatcgt aacaatcgat tgatgtggcg aaagattgca cgacttggcg cagacttcgc
     3661 tcgcccatcc tggccttgca acacctcacg caacgtcatt tgacgatgaa accatgcgcg
     3721 aacggatcac ggtcgtcgat gaagatcgtg ttcagtccgg tcatccgcgc ccatccgccg
     3781 atcgaaggca caatggcggt cttgccggcc accgaaacct ccttctcgac ctttccgtgg
     3841 aacagcgaac cgatgatgga ttcatgcacg aacgtgtcgc ccgccttcag cctgcccttg
     3901 gcgtgaagct gcgccatgcg cgccgaagtg ccggtgccgc aaggcgagcg gtcgatggcc
     3961 ttgtcgccgt aaaagacggc attgcgggca tcagcgccct cgacggtcgg cgcgccggtc
     4021 cacagcatat gcgaaagccg gttgatgcct gggttttcgg gatgcgcgaa gctgtatttc
     4081 tcattgaggc gctggcgcac caccgggctc cacgcgatga gatcgccggc cgaataatcg
     4141 gccatgtcgc ggtagttctt ctgcggatcg acgatggcgt agaagttgcc gccataggcg
     4201 acgtcgacgg tgatttcgcc aagctgcggg cattccaccg tcagcccctc ggcatagagg
     4261 aaggacggca cgttggtgat gcgcacttcc tcgacatatt cgccgacctg tttgtattcg
     4321 gcggtcacca ggccggccgg cgtgtcgagc cgcagcatgc ccggcgtctt cggcttgatc
     4381 agcccctgct cgatggccat cgtcaccgtg ccgatggtgc cgtggccgca catgggcagg
     4441 cagcccgagg tttcgatgaa caggatggcg atgtcgcaat cctcgcgcgt cggcggatag
     4501 aggatcgagc cggacatcac gtcatggccg cgcggctcga acatcagccc ggtgcggacc
     4561 cagtcatatt cggccaggaa atgcgcgcgc cgttccatca tggtcgtgcc ttcgagcagc
     4621 ggcccgccgc cggccaccag ccgcaccgga ttgccgcagg tatggccgtc gatacagaag
     4681 aaggattttt tggccattgc cgcctgcctt agaaacgttg gggactgaaa ggagaaagat
     4741 cgagcgcctc agcctgccct gaaacgagat cgcggatcaa gcggccggtt gccgcggcct
     4801 gggtcaggcc gagatgcccg tggccaaagg catagagcac gcacggatga ctgggcgcac
     4861 ggccgatgac gggccgcgaa tccggcagcg acgggcgaaa gcccatccat tcgcgcccgc
     4921 cgcttgcgtc cagtcccggc aggaatttct gcgccttggc cagcagtgcc ctcgaacgcg
     4981 cataattggg cgcgcgctcg atgccgccaa gctcgacggc gccgccaatg cgcaggccgg
     5041 tcgaaagcgg cgtgatcacg aagccatggc ccgagaagat cagctgccgc ttcacctcat
     5101 aggcggatgc cggcagggta gtgttgtagc cgcgctcggt ttcaagcgga atgcggtcgc
     5161 caaggtttcg cgccaagaga tgcgaccatg cgccggcggc cagcacaacc agcttcgcct
     5221 tccgcaccgc gccaccggcc agcgtcagcg tggcgcccgc ctcaaccgcg tcgatgcgct
     5281 cgatgcgcgc ccgctcgaac cgcgcgcctt tcgtctccgc ataggcccag atcgccttgc
     5341 ccagcagctt cggatcggcc accgtcttcc agccgggaac gaaggtgccc ttgatgaagc
     5401 gcggcgacag gccgggctgg agatcggcca gttccctgcc ctcgacatgg cggtagccga
     5461 tgccgaaacg gtcgcgcgcg gcccaaccgg acaacgaggc gcgaaactcc ggctcgcttt
     5521 catagagttc gagcgaaccg tcctcgcgc
//
LOCUS       DASYCS010000463         4652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_3188675,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000463 DASYCS010000000
VERSION     DASYCS010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 4652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4652
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3188675"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="VGM46_14380"
     CDS             <1..248
                     /locus_tag="VGM46_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006200757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193829.1"
                     /translation="RRAWFFGLLGATYLLDVVDTLLKGEAHFARFGHEYLFRTPVFVA
                     LCIIAAYTSNRRYHIAFVAVALVYQAWWIMRLFDTIT"
     gene            317..757
                     /locus_tag="VGM46_14385"
     CDS             317..757
                     /locus_tag="VGM46_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006325969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3995 domain-containing protein"
                     /protein_id="HEY4193830.1"
                     /translation="MIVLAFALSFVLLLITALHVYWGIGGIWPGTDAASCARAVCGFE
                     GMDEMPPPSSSFAVAACLVLATLWPIALVGVFATPFPDIGLALVAGLIGLGFVGRGIA
                     GYVPAWRRLTPEQPFATYDVRYFSPVSLLIGLGFIVLAVTQFPA"
     gene            836..1603
                     /locus_tag="VGM46_14390"
     CDS             836..1603
                     /locus_tag="VGM46_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015314496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase and adducin N-terminal
                     domain-containing protein"
                     /protein_id="HEY4193831.1"
                     /translation="MGVAALKNQKPTNLPFHEERVDLACAFRWTARLNMHEAVANHFS
                     LAVNDDGTQFLMNPNQAHFSRIKASDLLMIDANDPATLSGPDAPDPTAWGLHGAIHRN
                     VRHARCVMHVHSIHATVLASLADSTLPPIDQNTAIFYNRHVVDAHYGGLAFEEEGERC
                     SALLTDPKVKVMVMGNHGVLVLGDTVADAFNRMFYFERAAETYIKALWTGRPLRLLSD
                     AIAEKAAREQEDYPGQAERHLSELKAILDEQEPAYRN"
     gene            complement(1610..2317)
                     /gene="hisG"
                     /locus_tag="VGM46_14395"
     CDS             complement(1610..2317)
                     /gene="hisG"
                     /locus_tag="VGM46_14395"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEY4193832.1"
                     /translation="MSITLALPSKGRLKEQALEVLAKAGLCVSLPDDSRTYRARVEGR
                     ADIEVAFLSASEIAGEIGQGAVDLGITGEDLLRENLADWESRIEIAARLGFGHADVVV
                     AVPEIWLDVETMADLDDVAADFRQRHGRRLRIATKYWRLTQQFFSQKHGIQVYRIVES
                     LGATEGAPAAGSADVIVDITSSGSTLRANHLKVLGDGVVLASQACLAASRKTRSEADE
                     AALRDIAQRVSLAITGS"
     gene            complement(2314..3435)
                     /locus_tag="VGM46_14400"
     CDS             complement(2314..3435)
                     /locus_tag="VGM46_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEY4193833.1"
                     /translation="MTSRPAIAEAIAGFFVERSTHAVEVAVLQPADPFLDMAGEDLRR
                     RIFLTESETGQSLCLRPEFTIPVCLDHIASQAGTPRRYSYLGEVFRQHRDGGNEFFQA
                     GIEDLGDGDMARADARLLADAHALLALALPGRRLAVTLGDQSVFEAVLAALGLPRGWR
                     MRLARAFGSQAMLETALADLANPPRNGQLSSVVAALVADGDAAKLAEHIAGGMEQAGL
                     SASSGRAPAEIARRLIEKAELRSVRLSKDAFSALKTFLAIDVPLADAPRALEQFAADA
                     GLALGNALQNFAARAEALGGHGLSVESIRYDAAFGRPLDYYTGLVFEITASLGDAVVL
                     AGGGRYDRLLTLLGAKTPIPGVGFSVWLDRVEALRGVAP"
     gene            complement(3432..>4652)
                     /locus_tag="VGM46_14405"
     CDS             complement(3432..>4652)
                     /locus_tag="VGM46_14405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008125529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HisS family protein"
                     /protein_id="HEY4193834.1"
                     /translation="PFRSYRAGWVFRNEKPGPGRFRQFMQFDADTVGTPGVAADAEMA
                     MMMADVMEALGIARGDYVIRVNNRKVLDGVLEAIGLGGDDNAGRRLTVLRAVDKLDKV
                     GAEGVRDLLGKGRMDESGDFTKGAGLDDKQMERLGGSFMFLLPNEGQTDDEYTEAFFR
                     DLRSTVADTATGQEGVSELEQISALCRSSGYLRDRIRIDPSVVRGLEYYTGPVYEAEL
                     LAEIPNDEGKIVRFGSVGGGGRYDGLVSRFRGEPVPATGFSIGVSRLMTALKNLGKLD
                     AADVVAPVVVLVMDKDTESLGRYQKMVAELRAAKIRAEMYLGGAGMKAQIKYADRRGS
                     PVVVIQGGDERAQGVVQIKDLIEGARQASAIAGHAEYKEARPGQVTVPEADLVAEVQK
                     ILAAQAQDRAKASK"
BASE COUNT          776 a         1588 c         1455 g          833 t
ORIGIN      
        1 cgcggcgcgc ctggttcttc ggactgcttg gcgcgaccta cctgctcgac gtggtggata
       61 cgcttttgaa gggcgaggcg catttcgccc gtttcggcca cgaatatctc ttccgcacac
      121 cggttttcgt cgccctgtgc attatcgccg cctatacgag caatcgccgc tatcacatcg
      181 ccttcgtggc ggtggcgctg gtctatcagg cgtggtggat catgcggctc ttcgacacca
      241 tcacctgacg gccattgggt ggcggcggcg aatggtctag gaacggtcgt cgccaccgct
      301 gctacaggcc ctgtccatga tcgttcttgc cttcgccctc tccttcgtgc tgctgcttat
      361 caccgcgctg catgtctatt ggggcattgg cggtatctgg ccgggaacgg atgcggcctc
      421 ctgcgcgcgc gccgtctgcg gcttcgaagg catggacgag atgccgccgc cctcgtcttc
      481 tttcgcggtc gccgcctgcc tggtgctggc gacgctttgg ccgatcgcgc tggtgggcgt
      541 gtttgcgacg ccctttcccg acatcgggct ggcgttggtt gccgggctga tcgggcttgg
      601 cttcgttggg cgcggcatcg ccggctatgt gccggcatgg cggcggttga cgcccgaaca
      661 gcctttcgcg acctatgatg tgcgctattt ctcgccggtc agcctgctga tcgggcttgg
      721 cttcatcgtg ctcgccgtca cgcaatttcc tgcataacgc gcgctgcacc aaccaaaatc
      781 gattgcagca gacgatgggc cacgccattc ttcccgccac aagggaggaa tttcaatggg
      841 tgtcgccgcg ctgaagaacc aaaagccgac caacctgccg ttccacgaag agcgcgtcga
      901 tctcgcctgc gccttccgct ggacggcgcg gctcaacatg catgaggcgg tggccaatca
      961 tttctcgctg gccgtcaacg atgacggcac ccaattcctg atgaacccga accaggcgca
     1021 tttctcgcgc atcaaggcca gcgatcttct gatgatcgac gccaacgacc ccgccacctt
     1081 gtctggcccc gacgcgccag acccgaccgc ctgggggctg cacggcgcca tccatcgcaa
     1141 tgtgcgccac gcgcgctgcg tcatgcatgt gcattccatc cacgcgaccg tgcttgcctc
     1201 gctggccgat tcgacgctgc cgcccatcga ccagaacacg gcgatcttct acaaccgcca
     1261 tgtcgtcgac gcccattatg gcgggctggc cttcgaggaa gagggcgagc gctgctcggc
     1321 cctgttgacc gacccgaagg tcaaggtgat ggtcatgggc aatcatggcg tcctggtgct
     1381 cggcgacacc gtcgccgacg ccttcaaccg catgttttac ttcgagcgcg ccgccgaaac
     1441 ctacatcaag gcgctgtgga ccggccggcc gctgcgcctg ctttccgatg cgattgccga
     1501 gaaggccgcg cgggaacagg aagactatcc cggccaggcc gagcggcatc tgtcggaatt
     1561 gaaggcgatc ctcgacgagc aggagcctgc ataccggaac tgaccggatt cagctgccgg
     1621 tgattgccag cgacactctt tgcgcgatat cgcgcagcgc cgcctcgtcg gcctcgctgc
     1681 gcgtctttcg cgatgcggcc aggcaggcct gcgaggccag cacgacacca tcgccaagaa
     1741 ccttcagatg attggcgcgc agggtcgagc cgctcgacgt aatgtcgacg atgacatcgg
     1801 cggagccggc ggccggcgcg ccttccgtcg cgcccaggct ttcgacgatg cgatacacct
     1861 ggatgccgtg cttttgcgag aagaactgct gtgtcagccg ccagtatttg gtggcgatcc
     1921 tgagccggcg gccgtggcgc tggcggaaat ccgcggcgac atcatcgaga tcggccatcg
     1981 tttcgacgtc cagccaaatc tcgggcaccg caaccaccac atcggcatgg ccgaagccga
     2041 gccgggccgc gatctcgatc cggctttccc aatcggccag gttctcgcgc agcaaatcct
     2101 cgccggtaat gccgagatcg accgcgccct gccctatctc gccggcaatt tccgacgccg
     2161 acaggaatgc cacctcgatg tcggcgcggc cctcgacgcg ggcgcgatag gtgcgcgaat
     2221 cgtccggcag gctgacgcaa aggccggcct tggccagcac ttccagcgcc tgttccttca
     2281 gccggccttt cgaaggcaat gcaagcgtga tgctcatggg gcaactcccc gcagcgcttc
     2341 gacacggtcg agccagacgg aaaatccgac gccgggaatg ggcgttttgg cgcccagcag
     2401 cgtcaacaac cggtcgtagc ggccaccgcc ggccagtacg acagcgtcgc ccagcgatgc
     2461 ggtgatttcg aacaccaggc cggtataata atcgagcggg cggccgaagg cggcatcgta
     2521 gcggatgctt tccaccgaca ggccatggcc gccaagcgcc tcggcgcgcg ccgcgaaatt
     2581 ctgcaaggcg ttacccagcg ccagtccggc atcggcggcg aattgttcga gggcgcgcgg
     2641 cgcatcggcc agcggcacgt cgatggccag aaaggtcttc agcgccgaga acgcgtcttt
     2701 cgacagccgc acgctgcgca attcggcctt ttcgatcagc cggcgggcga tttcggccgg
     2761 cgcgcggccg gaagacgccg acaggccggc ctgttccatg ccgccggcaa tatgttcggc
     2821 aagtttcgcc gcatcgccat cggccaccag cgccgcaacg acgcttgaaa gctggccgtt
     2881 gcgcggcggg ttggcgaggt cggccaatgc cgtttccagc atcgcctgcg aaccgaaggc
     2941 acgcgccagg cgcatgcgcc agccgcgcgg caggccgagc gccgccagca ccgcctcgaa
     3001 caccgactgg tcgcccagcg tgacggcaag ccgccggcca ggcagcgcca aggccagcaa
     3061 ggcgtgggcg tcggcgagca accgggcgtc ggcgcgcgcc atgtcgccgt cgcccaaatc
     3121 ctcgatgccc gcctgaaaga actcgttgcc gccgtcgcgg tgctggcgga atacctcgcc
     3181 cagataggaa tagcggcgcg gcgtgccggc ctggctggcg atatgatcga ggcagacggg
     3241 aatggtgaat tcgggccgca ggcacaggct ttgcccggtt tcgctttcgg tcaggaagat
     3301 gcggcggcgc aaatcctcgc ccgccatgtc gaggaacggg tcggccggct gaagcaccgc
     3361 cacctcgacc gcgtgcgtgc tacgctcgac gaagaacccg gcaatggctt cggcaatcgc
     3421 cggtcgggag gtcactttga cgctttcgcc cgatcctgcg cctgcgcggc aaggattttt
     3481 tgcacttccg ccacaaggtc ggcttccggc accgtcacct ggccggggcg ggcttccttg
     3541 tattcggcgt ggccggcaat ggccgaagcc tggcgcgcgc cctcgatcaa atccttgatc
     3601 tgcacgacgc cctgcgcgcg ctcgtcgccg ccctggatga cgacgaccgg gctgccgcgc
     3661 cggtcggcat atttgatttg cgccttcatg ccggccccgc cgagatacat ttcggcgcgg
     3721 attttggccg cgcgaagctc cgccaccatc ttctggtagc ggccgaggct ttcggtgtcc
     3781 ttgtccatga ccagcaccac aacgggcgcg accacatcgg cagcgtcgag cttgccgagg
     3841 ttcttcagcg ccgtcatcag ccgcgagaca ccgatggaaa agcccgtcgc cggcaccggt
     3901 tcgccgcgaa agcgcgaaac cagcccgtca taacgcccgc cgccgccgac cgagccgaag
     3961 cgcacgattt ttccctcgtc attggggatt tccgccagca gctcggcttc gtagacgggg
     4021 ccggtgtaat attcgaggcc gcgtacgacg gaggggtcga tgcggatgcg gtcccgaaga
     4081 tagccagatg aacgacaaag cgccgaaatc tgctctagtt cgctgacgcc ctcttggccc
     4141 gtagctgtgt cggccacagt tgaccgcagg tcccggaaaa acgcttccgt atattcgtcg
     4201 tccgtttgcc cctcgttcgg aagcaaaaac ataaacgacc cacctagccg ctccatctgc
     4261 ttgtcatcca gcccggcgcc cttggtaaaa tcgccgctct cgtccatgcg gcccttgccg
     4321 agcagatcgc gcacgccttc cgcgccgacc ttatctagct tgtcgacggc gcgcagcacg
     4381 gtcaggcggc ggccggcatt gtcgtcgccg ccaaggccga tggcctccag cacgccgtcc
     4441 agcaccttgc ggttgttgac gcggatgacg tagtcgccgc gcgcgatgcc cagcgcttcc
     4501 atcacgtcag ccatcatcat cgccatctcg gcgtccgccg cgacgcccgg cgtgccgacc
     4561 gtgtcggcat cgaactgcat gaactggcgg aagcggcccg gaccgggctt ctcgttgcgg
     4621 aacacccagc cggcgcgata ggaacggaag gg
//
LOCUS       DASYCS010000464         1847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2607473,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000464 DASYCS010000000
VERSION     DASYCS010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2607473"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1284)
                     /locus_tag="VGM46_14410"
     CDS             complement(1..1284)
                     /locus_tag="VGM46_14410"
                     /EC_number="2.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018427417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase"
                     /protein_id="HEY4193835.1"
                     /translation="MSQRTPGFDTLAVHAGAKPDPATGARAVPIYQTTSFVFEDADHA
                     ASLFGLKAFGNIYTRIMNPTQAVLEERVAALEGGTAALATASGQAAHLLVFHTLLETG
                     DNFVAASKLYGGTINQFGHAFKNFGWEVRWADVNDPASFESQIDGRTKAIHVESLANP
                     GGEFVDIEKIAAIARRHGLPLVVDNTLASPYLLRPIEHGADIVVHSMTKFIGGHGNSV
                     GGIIIDGGTFDWSKSGKYPMLSSPRPEYGGMVLHETFGNFAFAIATRVLGLRDLGPAI
                     SPFNAFLILTGLETLSLRMQRHCDNALAVAEWLSKQPKVAWVSYPGLKGDKNNALQQK
                     YSPKGAGAVFTFGVKGGYESGIKLVESLELFSHLANIGDTKSLVIHPASTTHRQLSEE
                     QRVAAGAGPEVVRVSIGIEDFADIIADLEQALAKA"
     gene            complement(1433..>1847)
                     /locus_tag="VGM46_14415"
     CDS             complement(1433..>1847)
                     /locus_tag="VGM46_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEY4193836.1"
                     /translation="VLKYLLAKGFSVFPVNPGHAGKEILGRMTYAKLADIPEPIDMID
                     IFRASASVPPIVDEALALDPLPKIIWMQLTVRNDEAAAKAEAAGIKVVMNRCPKIEYG
                     RLSGEIGWNGVNSGVLSSKKPVMRGGFQSFGIRQP"
BASE COUNT          372 a          530 c          603 g          342 t
ORIGIN      
        1 tcaggctttg gccagagcct gttccagatc ggcgatgatg tcggcgaaat cctcgatgcc
       61 gatcgacacg cgcaccactt ccgggccggc accggcggca acgcgctgct cttccgaaag
      121 ctggcggtgc gtggtcgaag ccgggtggat gaccagcgac ttggtgtcgc cgatgttggc
      181 gagatgcgag aatagttcca gcgattcaac cagcttgatg ccggattcgt agccgccctt
      241 gacgccgaag gtgaacaccg cgcccgcccc tttcggcgaa tatttctgct ggagcgcgtt
      301 gttcttgtcg cccttgaggc cggggtaaga aacccatgcc accttcggtt gcttcgaaag
      361 ccattcggcg acggccagcg cattatcgca gtgccgctgc atgcgaagcg acagcgtttc
      421 aagaccggtc aggatcagga aagcgttgaa cggcgaaatc gccgggccaa gatcgcgcag
      481 gccaagcacg cgcgtagcga tggcgaaggc gaaattgccg aatgtttcat gcagcaccat
      541 gccgccatat tcggggcgcg gcgacgacag catcggatat ttgccggatt tcgaccagtc
      601 gaaggtgccg ccatcgatga tgatgccgcc gacggaattg ccatggccgc cgatgaattt
      661 cgtcatcgaa tgcacgacga tatcggcgcc atgctcgatg gggcgcagca gatagggcga
      721 agccagcgtg ttgtcgacga ccagcggcag tccgtgcctg cgcgcgatgg ccgcgatctt
      781 ttcgatatcg acgaattcgc cgccgggatt ggccaggctt tcgacatgga tagccttggt
      841 cctgccgtcg atctggcttt cgaaactggc cgggtcgttg acgtcggccc agcggacttc
      901 ccagccgaaa ttcttgaagg cgtggccgaa ctggttgatg gtgccgccat agagcttgct
      961 ggcggcgacg aaattgtcgc cggtttccag cagcgtgtgg aagacgagca gatgcgccgc
     1021 ctggccggac gccgtggcga gcgcggccgt gccgccttca agggctgcga cacgctcttc
     1081 cagcaccgcc tgagtcgggt tcatgatgcg ggtgtagatg ttgccgaacg ccttcaggcc
     1141 aaacagcgag gcggcatggt cggcgtcctc gaacacgaag gaggtggtct ggtagatcgg
     1201 cacggcgcgc gcgccggtcg ccgggtcggg ctttgcgccg gcatggacgg caagcgtatc
     1261 gaaaccgggc gtgcgctgtg acatgaaaaa actcctccag gaaccgtgcg gaaagccggc
     1321 cgcaactttg gcggagggtg gcggcaattg caaaggggaa aatgcctaac ctcgccgcgc
     1381 aatggaataa gcgcggcgtc aaacggtcat cgggtggaat aaaattcgcg gcctaaggct
     1441 ggcgaatgcc gaaactctgg aagccgcctc gcatgaccgg ctttttggac gatagaacac
     1501 cggaattgac gccattccag ccgatctcgc cggacaaccg gccatattcg atcttggggc
     1561 agcggttcat caccaccttg atgccggcgg cctcggcctt ggcggcagcc tcgtcgttgc
     1621 gaaccgtcaa ctgcatccag atgatcttcg gcagcgggtc gagcgcaagc gcctcatcga
     1681 cgatgggcgg cacagaagcc gaggcacgga aaatatcgat catgtcgatg ggttcgggaa
     1741 tgtcggcgag tttggcatag gtcatgcggc caagaatttc cttgccggca tggccgggat
     1801 tgaccggaaa gaccgaaaaa cccttggcga gcagatattt cagcacg
//
LOCUS       DASYCS010000465         2757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1301479,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000465 DASYCS010000000
VERSION     DASYCS010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301479"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VGM46_14420"
     CDS             complement(<1..287)
                     /locus_tag="VGM46_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193837.1"
                     /translation="MIWIAYLIIALVVLQRFGELVLANRNTQRLKARGGVEIGAEHYR
                     FIVLLHMAWLMAVLWLLPAPLVIYWPWIVVFVVLQAARVWVIASLGRYWTTR"
     gene            complement(284..1336)
                     /locus_tag="VGM46_14425"
     CDS             complement(284..1336)
                     /locus_tag="VGM46_14425"
                     /inference="COORDINATES: protein motif:HMM:NF012421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III polyketide synthase"
                     /protein_id="HEY4193838.1"
                     /translation="MRPRLLALKSAVPPYVIAQTEAATYARSLFAQVRDIARLIPVFQ
                     NTGIERRYSCVPIKWYLGDHGWMDRTQLYVENAVNLLETVARGLLDEAGLRTNDIDSI
                     VVVSTTGVATPSLDALLMERMGLRRDIQRLPIFGLGCAGGLIGLSRAADLASARPGSR
                     VLFLVVELCALTFRKNDISKSNIVATALFGDGAAGAILSTDGEGPLLGAAGEHTWPNS
                     LDVMGWDMAEDGLKARFATSIPGLVASDFRPLLNEFLARNGIAFSEIDGFACHPGGAK
                     VLDALEDALELPRGDLKDSRSILRDYGNMSAVTVLFVAERMDVRRKSQRTLLTALGPG
                     FSAAFLMLDGQGLEST"
     gene            1407..1937
                     /locus_tag="VGM46_14430"
     CDS             1407..1937
                     /locus_tag="VGM46_14430"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HEY4193839.1"
                     /translation="MKDQLGFGALTHEVCKKRRKCGVLNTFAELKSSACACKSDLSRG
                     RLKKETSMSYGKIAAASLLAVGMLSSQAFAAGDAVKGKAVFARCAVCHNVEKDGGNGV
                     GPNLFGISGRKSASVDGFSYSGPMKAANIVWNDDNLSKWIAGPAKMIPGTKMAFGGIS
                     QKSQIADLVAYLDTLK"
     gene            2153..>2757
                     /locus_tag="VGM46_14435"
     CDS             2153..>2757
                     /locus_tag="VGM46_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEY4193840.1"
                     /translation="MKVLVPVKRVIDYNVKVRVKSDQTGVDLANVKMSMNPFDEIAVE
                     EAVRLKEKGKASEIIVVSIGPQQASETIRTALAMGADRGILVKSDSEVEPLAVAKILK
                     AIVEAEKPELVITGKQAIDDDSNQTGQMLAALLGWSQGTFASKLAIEGDSVTVTREVD
                     GGLQTVKLKAPAIVTTDLRLNEPRYASLPNIMKAKKKPIEE"
BASE COUNT          612 a          840 c          844 g          461 t
ORIGIN      
        1 cgcgtggtcc aatagcgacc caggctggcg attacccaga cgcgcgcggc ctgcaacacg
       61 acaaagacca cgatccaagg ccaatagatc accagcggtg ccggcagcag ccacagcacc
      121 gccatcagcc acgccatgtg caacagcacg atgaaacgat aatgctcggc accgatctcg
      181 accccgcccc gcgccttcaa tctctgggtg ttgcggttgg cgaggacaag ctcgccgaac
      241 cgctgcagca ccaccagcgc gatgatgaga taggcgatcc agatcacgtg ctctccaacc
      301 cctggccgtc cagcatcagg aaggcggcgg aaaaaccggg cccgagcgcg gtcagcagcg
      361 tgcgctggct tttgcgccgc acgtccatcc gctcggccac gaacagcacc gtcaccgccg
      421 acatgttgcc atagtcgcgc aggatcgagc ggctgtcttt caagtctccg cgcggcaatt
      481 ccagcgcgtc ttccagcgca tccagcacct tggcgccgcc gggatggcag gcaaagccat
      541 caatctcgga aaatgcgatg ccgttgcggg caagaaattc attcaacagc ggacggaaat
      601 cactcgcgac cagaccggga atactggtgg cgaagcgcgc cttcagcccg tcctcggcca
      661 tgtcccagcc catcacgtcc agcgaattgg gccaggtatg ctcacccgcc gcgcccaata
      721 gcggaccttc gccatcggta gaaaggatcg ccccggccgc gccgtcgccg aacaaggcgg
      781 tggcgacgat gttgcttttg gagatgtcgt tcttgcgaaa ggtaagcgcg caaagctcga
      841 ccaccaggaa caacaccctg ctgccgggcc gggcgctggc gagatcggcg gcgcgcgaca
      901 gaccgatcaa cccgccggca caacccagcc cgaaaatcgg cagacgctgg atgtcgcgcc
      961 gcagccccat ccgctccatc agcagcgcat ccaggctggg cgtggcgacg ccggtggtgg
     1021 aaaccaccac gatactgtct atgtcgttgg tcctgagacc ggcttcgtcc agcagtcctc
     1081 gcgccaccgt ctctagcaga tttacagcgt tttcgacata gagctgggtg cggtccatcc
     1141 agccatggtc gccgagatac catttgatcg gcacgcagga ataacgccgc tcgatgccgg
     1201 tattctggaa caccggaata agccgcgcga tgtcgcgcac ttgcgcgaaa aggcttcggg
     1261 cgtaggtggc ggcttcggtt tgggcgatga cataaggggg aacggccgat ttcaaggcca
     1321 acaggcgggg acgcatcgat cctcgtttac gcagtaactg cgtttttctt attgccgagc
     1381 cccgctcctt cataggagcg cggcagatga aagaccagct tgggttcggc gcgcttacgc
     1441 acgaagtgtg caaaaagcgg cggaaatgcg gcgttttgaa cacttttgct gagttgaaaa
     1501 gcagtgcgtg cgcatgtaaa tctgacctca gcaggggccg actgaaaaag gaaacgtcga
     1561 tgtcgtatgg gaaaattgcc gccgcgtcgt tgctcgcggt gggcatgctg tcgagtcagg
     1621 ccttcgcggc cggggacgcg gtcaaaggca aagccgtgtt cgcgcgctgc gccgtttgcc
     1681 acaacgtgga aaaggatggc ggcaatggcg ttggccccaa cctgttcggt atttcgggcc
     1741 gcaagtcggc gtccgtcgat ggcttttcgt attcgggccc gatgaaagcc gcgaacatcg
     1801 tctggaacga tgacaatctg tcgaagtgga ttgccggtcc cgccaagatg atcccgggca
     1861 ccaagatggc gtttggcggc atctcccaga aatcccaaat cgccgacttg gtggcctatc
     1921 tggacacatt gaaataggcc agtctgtggc gcgatgacgc agcacggcgt tgctgcgacg
     1981 cagcagaact gaagaagttc ctgaacccgg cacgtggcga acgtgccggg ttcattgaca
     2041 tatggcgact ccggtgccta gtttttgcgc cgcacaagag cgccacaaca gcgaaaaagg
     2101 gcgcgctccg gcaagaggtt caaaaccgac ccaatgggga tccgataacg ccatgaaagt
     2161 gctggtgccc gtgaagcggg tgatcgacta caacgtcaag gtccgcgtca aatcggacca
     2221 gaccggcgtc gatctcgcca atgtcaaaat gtccatgaac cctttcgacg aaatcgccgt
     2281 cgaggaagcc gtccgcctga aggaaaaggg caaggcgagc gaaatcattg tcgtttccat
     2341 cggaccgcag caagccagcg aaaccatccg caccgcgctg gccatgggcg ccgatcgcgg
     2401 catcctggtg aagagcgaca gcgaggtcga acctctggcc gtcgccaaga tcctcaaggc
     2461 catcgtcgaa gccgaaaagc ccgagctggt gatcaccggc aagcaggcga tcgacgacga
     2521 cagcaaccag accggacaga tgcttgccgc cttgcttggc tggtcgcagg gcacgttcgc
     2581 gtcgaagctc gccatcgagg gcgacagcgt gacggtgacg cgcgaagtgg acggcggctt
     2641 gcagacggtg aagctgaaag ccccggcgat cgtgacgacc gatttgcggc tgaacgaacc
     2701 gcgttacgct tcgttgccga acatcatgaa ggcgaagaag aagccgatcg aggaaaa
//
LOCUS       DASYCS010000466        21195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_143339,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000466 DASYCS010000000
VERSION     DASYCS010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 21195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21195
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143339"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /gene="ccmI"
                     /locus_tag="VGM46_14440"
     CDS             complement(<1..279)
                     /gene="ccmI"
                     /locus_tag="VGM46_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008839532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HEY4193841.1"
                     /translation="MLFWVIAAVLTLGASLAVLLPLAARPKGMGEGSSHDLEVYRDQL
                     SELDRDVARGMIHASQADQARAEIARRILRLDNAPSQPATAGSSRAMRW"
     gene            complement(409..855)
                     /locus_tag="VGM46_14445"
     CDS             complement(409..855)
                     /locus_tag="VGM46_14445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193842.1"
                     /translation="MKIRAAFVSGLLIVSGCASTGLGKGGGGVLSPAPSSQQSGGATV
                     AKAIISAMDGGLVGGKIGAGLTDQEKRSGLEAEYKALEYTASGQTVAWKSGSSSRYGE
                     VVAAQPYRVGSQDCRQYTHTVFTGGQSASARGTACRNADGSWTPLT"
     gene            complement(955..2292)
                     /locus_tag="VGM46_14450"
     CDS             complement(955..2292)
                     /locus_tag="VGM46_14450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY4193843.1"
                     /translation="MAFSTVWAILALIVIFTLITTLYRQASERGFDSLLSAHLFNLIG
                     SVGISDKAVLTGSPDLGDLRFSEPESGWYWQVQPASEGVTGERHSSSMTEDIPSPSAA
                     EVPFNTAFQRTYTTDGIDGEELQVFESEFALDNKNHAARFRVMGNQSELEQEIAAFQR
                     RLATYLSLFGFGMISINAVAILLGLQPLRRVGNALAMVREGTAQRLTGRFPVEIEPLA
                     NETNALIENNRRIVERSRTQVGNLAHSLKTPLAVLINESRALGGAKGQLIAEQAASMQ
                     KQVDHYLQRARVAAQRDSVVYRTPVAPLMGRMVRVVQKLNPQAGLSLSMADEDIVFAG
                     EREDLEELIGNLLENAMKWAKGAVLVSVARLPDESAADRFEIAIEDDGPGIPEEKARE
                     ALKRGARLDETKPGSGLGLAIVAELVTEYGGTLALERSSLGGLKAVVRLRSLS"
     gene            complement(2375..3040)
                     /locus_tag="VGM46_14455"
     CDS             complement(2375..3040)
                     /locus_tag="VGM46_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010915044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY4193844.1"
                     /translation="MRVLVVEDDKDLNRQVSDALVDAGYVVDRAFDGEEGHFLGDTEP
                     YDAVVLDIGLPQMDGISVVERWRRNGRKMPVLILTARDRWSDKVSGIDAGADDYVAKP
                     FHIEEVLARIRALIRRAAGHASSELTCGPLRLDTKASKADVAGVPLKLTSHEFRLLAY
                     LMHHMGKVVSRTELVEHLYDQDFDRDSNTIEVFVGRLRKKMGIDMIETVRGMGYRMRE
                     PEA"
     gene            complement(3130..3441)
                     /locus_tag="VGM46_14460"
     CDS             complement(3130..3441)
                     /locus_tag="VGM46_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193845.1"
                     /translation="MNAFFTLFRRAVLTICSAGLLLSHATAMPLAGLSVAQGDSRAVV
                     KVASDCYAIGQQVAEQNGGTLAKASQSTQGGQPVCVIVVLVPGKEGQRPRRSEIVVPQ
                     N"
     gene            complement(3565..3771)
                     /locus_tag="VGM46_14465"
     CDS             complement(3565..3771)
                     /locus_tag="VGM46_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008839527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193846.1"
                     /translation="MTTSKPWYLSRTIWASIVSVLTGTAGLTGLPLDGLDNGALTDTL
                     LQIISAASGLIAIFGRLSAKDRIG"
     gene            complement(3807..7703)
                     /locus_tag="VGM46_14470"
     CDS             complement(3807..7703)
                     /locus_tag="VGM46_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase/phage tail family protein"
                     /protein_id="HEY4193847.1"
                     /translation="MATILLQAAGAYLGGFLGSVGGAIGSAAGAVAGYVVDRALINST
                     QHIEGPRLTSARPFTAEEGVSIARLYGTARIGGILVWATRFEESSATSRQGKLGPKVT
                     EYSYFANVAFLLCEGEIAGIRRVWADGREIDREKIEMRVYRGSADQPADPLIEAKQGS
                     GNTPAYRGVAYVVLERFDIGEFGNRVPQLQFEVIRPVGRLNGKIKAISLIPGAIEYGL
                     SAELVSRERRPGDQEAVNRHVLFAGTDIAASLDELQMLCPSLQHVALVVSWFGDDLRA
                     GHCRIEPAVTTADEQGLSSEWRVSATAREEAKVVSVHDGGGAFGGTPSDASVLSAIAE
                     LKARGLSVTLYPFILMDVAEGNAFPSPYGGSVQPSYPWRGRITADPAPMQPGSADKTA
                     AARAQVSAFCGTAQPSQFEQANDTVAFIGAPGEWSYRRMILHYAHLAVKAGGVNAFLI
                     GSELRGLTTLRDEHGAFPFVEQLCGLAADVRSIVGAQTRLTYGADWSEYFGHHPEDGS
                     GDAYFHLDALWAHAAIDAVGIDNYMPLSDWRDNDHAGGNPDGFAGPYDPHGLTASIAG
                     GEGFDWYYASSEARQGRVRSPITDGAYGKPWVYRYKDIANWWSNPHHDRIGAVEVATP
                     TAWVAQGKPIWFTEVGCPATDKGPNQPNVFPDPKSVENASPYFSNGGRSDAAQQAFLQ
                     AHMDYWDQAQPDFEEARNPRSSVYDGRMVDATRIYPWSWDARPYPAFPLRGDLWSDSA
                     NFDRGHWLNGRLAYPSLAALINAILADHGQPPADTTDVEGTVEGYLVTDPTSARSAIE
                     PLTDLFDIIVREEADALVFRQAGATPDPTFYLTELVADEDGPLIETVRLPDHQLPAEA
                     TLTFLTALTEYQSASVRGTRLGAAGSRQQNLSFPGVLEVGQGKALLDDWLDRVWYERE
                     TITFSVAQPRVDVEPGSIVRLPASGSSSDFLVVQVEDGLVRKVSARQVARGTPAPSRP
                     SMLSGNAPAPILTGQPHALFLDLPAGIGADDPVTQFRAASWQKPWRSQSLFASPEDTG
                     FAFRSSLGKAASLGRLASALEPGFTGRIDRQGEILVELFDAQATSISMLQLLNGANAA
                     AIRSASGAWEIIQFSDAEEVSSQIWQLRGLLRGQLGTDDAMAEGAAVGSDFVMLDDAV
                     RPAGLLDGEVGLALNWRVGPSGSSLSSADFLTRNETGGIRSLLPLSPVHLKAGRVGDD
                     IKLSWIRRGRIDADKWESTEIPLGEEREEYQVDIAAPGGATVRTRIVSESEWTYPAAD
                     IATDFASPPAEIDVTVRQLSIRTGWGLPATRRLAVS"
     gene            complement(7717..8130)
                     /locus_tag="VGM46_14475"
     CDS             complement(7717..8130)
                     /locus_tag="VGM46_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEY4193848.1"
                     /translation="MTWVETPYLHQACRKGVGCDCLGLVRGIWRALYGFDLEAPGPYA
                     PDWAEAGGGEDLLAGMRRNFHELAPSNAKAGHVFVFRWRPNLPAKHAGILVEGHRFVH
                     AYQGAGAVMVSALVPQWRRRVAAAFSFPEFKDIST"
     gene            complement(8187..9077)
                     /locus_tag="VGM46_14480"
     CDS             complement(8187..9077)
                     /locus_tag="VGM46_14480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2163 domain-containing protein"
                     /protein_id="HEY4193849.1"
                     /translation="MSAYPQQLVDHLSREVTTVCNCWRLTRTDGDVTGYTDHDFALAF
                     DGAVFEPGSGFSASEARDTLGLAVDTVDVEGALSSQRIRDEDIAAGLYDGAKVETFLV
                     NWRKPQDFVLTRTATIGKMTRSDGRFVAELESLVSRLDQPNGRIVSRSCDAELGDARC
                     GFLLDQPGFQAQGAVQVLEAPDKIAVSGLGGFDGGWFSLGTLRWTTGGRAGRTERIVD
                     HRLTNGDAFLVLQQSVGPAIATGDGFTLAAGCDKNFATCKAKFDNALNFRGFPHLPGN
                     DAAYSYVVDGGNFDGGPVVP"
     gene            complement(9074..9724)
                     /locus_tag="VGM46_14485"
     CDS             complement(9074..9724)
                     /locus_tag="VGM46_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2460 domain-containing protein"
                     /protein_id="HEY4193850.1"
                     /translation="MSELSTFHDVLFPPGISFGATGGPERRNEIVALTSGREKRNARF
                     SQSRHHYDAGTGIKSLDDLHEVVAFFEARRGSLHAFRFRDPFDRKSCRPSQTPAAHDQ
                     PLGAGDGVTARFALLKTYGTGDDAYQRLIAKPVISSLRVAVDDVELTEAGAFSFDDAT
                     GEVVFAAGKIPGNGQGVTCGYEFDVPVRFNADRLEISLKAFKAGQIPSIPLIEVQL"
     gene            complement(9735..10298)
                     /locus_tag="VGM46_14490"
     CDS             complement(9735..10298)
                     /locus_tag="VGM46_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEY4193851.1"
                     /translation="MTVAIRADTEPFQNALESLERLSGNFGSQLSGALKSAAVSGREL
                     DDVLRRVGLNLAGMALTQGLKPLQSLTGSLFSGLLGGLSGILPFAKGGVPGSVVPFAS
                     GGVVSSPSYFPLGGNIGLMGEAGSEAILPLRRGPDGSLGVASAGGGGAVNVVFNVTAQ
                     DAASFRKSEAQITGMLARAVSRGTRTF"
     gene            complement(10303..10527)
                     /locus_tag="VGM46_14495"
     CDS             complement(10303..10527)
                     /locus_tag="VGM46_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rcc01693 family protein"
                     /protein_id="HEY4193852.1"
                     /translation="MAGAKAAFPWDDVMAIGFGLLRLSPGAFWAMTPIEFERAMSAVR
                     PARASPPGRADLAALMRAFPDPTAMETGFG"
     gene            complement(10533..10874)
                     /locus_tag="VGM46_14500"
     CDS             complement(10533..10874)
                     /locus_tag="VGM46_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009363046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gene transfer agent family protein"
                     /protein_id="HEY4193853.1"
                     /translation="MSANRRRGEISAELDGAPHRLCLTLGALAELEAAYAADDLAALV
                     ERFSTGRLSARDMIRIVGAGLRGAGEQVTDDAVGKMQSEGGAAGFAEIVADLLGTTFG
                     RAANDETGPNP"
     gene            complement(10871..11287)
                     /locus_tag="VGM46_14505"
     CDS             complement(10871..11287)
                     /locus_tag="VGM46_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009452183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HEY4193854.1"
                     /translation="MVAQKGKDLLLKLDSDGIGNFVTVAGLRSKRIAFNSETVDITDS
                     DSAGRWRELLAGAGVQRAAISGSGIFKDAQSDADIRARFFAGEISDWQLAVPGFGTVA
                     GPFQITSLEYTGAHDGEVTFEMALESAGSIEFAVAS"
     gene            complement(11338..11748)
                     /locus_tag="VGM46_14510"
     CDS             complement(11338..11748)
                     /locus_tag="VGM46_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007067386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HEY4193855.1"
                     /translation="MISPVADLQKTLFEALAGDNALAALLDGAKIFDHAPADVRFPYI
                     TFGRTSVYDWSTDTDSGTEQLFTLHIWSKAKGKKETLDIMEAVRARLDDGSLALDQHH
                     LVSLRFEYAESRYDDDQSVHHGLLRFRAVIEQSA"
     gene            complement(11729..11914)
                     /locus_tag="VGM46_14515"
     CDS             complement(11729..11914)
                     /locus_tag="VGM46_14515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193856.1"
                     /translation="MKLAMTLTMDGLIQALRRTAHDLADALEGGYASAAGEPEDEKLS
                     PQMQGHERRQEDDFTRR"
     gene            complement(11911..12246)
                     /locus_tag="VGM46_14520"
     CDS             complement(11911..12246)
                     /locus_tag="VGM46_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009452181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEY4193857.1"
                     /translation="MPVLFIDPGEFRARLALEACTTEADGLGGFTEIWTQAATLFAKI
                     EPLSAESVFGADQTLETVTHRITLRQRGGVASGMRFTRVDRVFDIVTVHDPDESGRYL
                     VCKVREAGA"
     gene            complement(12246..12815)
                     /locus_tag="VGM46_14525"
     CDS             complement(12246..12815)
                     /locus_tag="VGM46_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HEY4193858.1"
                     /translation="MTLIRTVGPTAEPVTLAEMKLNMRLDHDTDDDLIGGLIRAARED
                     VERSTGLALIDQNWRLVLDRWPRNGCATIPVYPVREILSVTAYGAEGEACLIDPATYL
                     ADTVSRPARLHFEASPRTLRALNGIEIDFVAGYGEAGVDVPDLLKRAIALLVLHWYEF
                     RTAFGPDDQPVSYPQVYERMIAPFRARRL"
     gene            complement(12959..14215)
                     /locus_tag="VGM46_14530"
     CDS             complement(12959..14215)
                     /locus_tag="VGM46_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009758214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEY4193859.1"
                     /translation="MNATHQPASIETKSASGEYFDLKDAFGDFMNTFEAFKESNDQKL
                     AQLEGRMGADTVTVDKVERISNALDEQKRAIDNLALKKIRPTLGRDGAVVSSEHKGAF
                     DAYMRSGDDRQLRALDTKAMSYGSGQDGGYLVPAETEVEIGKRLSVLSPIRSIASVRQ
                     VSSAVLKKPFSVSGPAVGWVAETASRPQTDTATLAELQFPTMELYAMPAATASLLEDT
                     VVDLDQWISSEVEAAFAEQEGAAFISGDGASKPKGFLSYATVAEASWAWGKIGYTLTG
                     VSGAMSADSPSDILIDTIYALKAGYRQNANWVMNRRTQAAIRKLKDADGNYLWQPPSV
                     AGQRAALMGFPLVEAEDMPDIGVDATPLAFGDFSRGYLVVDRAGVRVLRDPYSAKPYV
                     LFYTTKRVGGGVQDFDAIKLLKFGTA"
     gene            complement(14212..14907)
                     /locus_tag="VGM46_14535"
     CDS             complement(14212..14907)
                     /locus_tag="VGM46_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HEY4193860.1"
                     /translation="MRSEALERACERKFVDLVLDEVEADGSFAGYASLFGKVDLGKDA
                     VERGAFAKSLRVRGAAGIRMLFQHDPGEPIGVWTEIKEDARGLFVRGRLAKDVRRARE
                     VLSLMRGGALDGLSIGFRAVKARRDPASGVRHILEADLWEISVVTFPMLPDARIQTVK
                     GRRAKRLPTVREFELWLTRDAGLSRSDARGVIARGFANLVREREAAPGTVEGLAARIR
                     EATNLINQRGNTL"
     gene            complement(14897..15304)
                     /locus_tag="VGM46_14540"
     CDS             complement(14897..15304)
                     /locus_tag="VGM46_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6107 family protein"
                     /protein_id="HEY4193861.1"
                     /translation="MTDPSQPAWVWIAKGAGAVAGSAISLVYILPHGRREAASRFAVG
                     VVCGLVFGGTAGLKISSELSVENVIGPVELMLMGSAAASLCAWWALGFVVRALQQSSL
                     PDLFRKNDLFRRPDLLRENDLPGKKTSEDTHEK"
     gene            complement(15328..16509)
                     /locus_tag="VGM46_14545"
     CDS             complement(15328..16509)
                     /locus_tag="VGM46_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEY4193862.1"
                     /translation="MAWNWSWPRAAGNAGVRPENKSASAGGFIAFHAQGEARWTRRDY
                     ATLCREGFMRNPIVHRSVRLISETASAVPWLLYDGVVEIDDHPLLNLLERPNQRQAGA
                     TFLDALYGHLLLAGNAYVELIDTGNGAREMHLLRPDRVSVVTDASGWPTALDYRAGSE
                     RRTVPLGAGEGGGALHLTLFHPLDDHYGFPPLEAALMALDTHNSAGRWNKALLDNSAR
                     PSGALVYAPKDGGNLSDEQFARLKSELEDGYSGATRAGRPLLLEGGLDWKAMGMTPKD
                     MDFVEAKHAASRDIALAFGVPPMLLGIPGDNTFANYQEANRAFYRLTVLPLVARTAKE
                     LSGWLGAAFGGSLRLWYDADRIESLNADRGALWARIDAATFLTEDEKREAVGYRPRDL
                     V"
     gene            complement(16519..17799)
                     /locus_tag="VGM46_14550"
     CDS             complement(16519..17799)
                     /locus_tag="VGM46_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HEY4193863.1"
                     /translation="MRGGWSEKNLTLAELALIRGEWLEIARDAQYPLGSKPDSWLVMG
                     GRGAGKTRLGAEWVNGLVRGFSPFADRRHGRFALIGETLADVRDVMIEGPSGILTISR
                     NDRPRFEASRRRLLWDNGAVATIFSSEDPESLRGHQFEAAWCDEAAKWRYAEACFDML
                     QFGLRLGAQPRQIITTTPRPTKLMKRLLADPSVVVTRMRTDENAANLAPGFMKMVERR
                     YAGTRLGRQELGGEMIEDREDALWSRSMIEEVALAAAGPLGRIVVAVDPPASSRKTSD
                     ACGIVAAGLDGQGQVIVLADATLPTAKPQEWARQAVELFHKLQADCLVVEVNQGGEMA
                     TAVIRTVDPTVPVKAVRAQRGKWLRAEPIAALYQQGRVRHAGRFGELEDEMCDFGPNG
                     LSNGRSPDRVDALVWAVTELMPAWSAHPRIRDFI"
     gene            complement(17759..18151)
                     /locus_tag="VGM46_14555"
     CDS             complement(17759..18151)
                     /locus_tag="VGM46_14555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4193864.1"
                     /translation="MATAAGRSSAALRKRAEREGWRDAEMAMQLSSQEMRLRVLLDQQ
                     ITKAEISGNDTDASASADKARMDAISAVMRTLEKIGEITRSDTGAKENQARRDANIAD
                     VLKRIDERIIELAVGYARRLVGEKSDAG"
     gene            18525..20729
                     /locus_tag="VGM46_14560"
     CDS             18525..20729
                     /locus_tag="VGM46_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008839499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HEY4193865.1"
                     /translation="MVDPAEKQKKRGALAAFLLSLDARLDSALYETGFKAREFWEAAT
                     IFFRRFRVTGWRRGIVEILSEAFTIGAGGSVIMLALALPAFQDTTGDWRAQGDFAVTF
                     LDRYGNDIGQRGIIQRDSVPVDEMPDHVIKAVLATEDRRFFEHYGIDFLGLARAMSEN
                     VRANSVVQGGSSITQQLAKNLFLSNERTLERKIKEAFLSVWLECNLSKKEILQLYLDR
                     SYMGGGTFGIEAAADFYFGKSVKDLNLAEAAMLAGLFKAPTKYAPHVNLPAARARANV
                     VLSNLVDAGFMTEGQVLSARLHPADVVDRGEQKSPDYYLDWAFDEVKKIAAKSGQHSL
                     VAHTTFDANLQKAAEESLEFHLRQFGKEYRVAEGAIVVIETNGAVRAIVGGRDYGASQ
                     FNRATKALRQTGSSFKPYVYATAMEHGFTPDSVISGGPISWGGWSPHNYGGAAAGKVT
                     LTTAIVKSINTVPVRLAKDYLGIAPIKAMALSFGVESPLESHKTMVLGTSGMTVMDQA
                     TGYSVFAQGGMVGTRHGITQLVTHTGQVVYDYAKDAPPPRRVLSEKALKAMNTMLSQV
                     PVIGTARRAALPGIVVAGKTGTTQSYRDAWFVGFTGNYTAAVWLGNDDFSTTNNMTGG
                     TLPAMIWQRLMAYAHQNIDLKPIPGLEHPFIDAEVAAKAGEAEKKKAEQEAADTAASR
                     PPVLSSGTTRTLREMSHLFQSAPNLGNSANPAEPTNSANPAGPAKPETLSAL"
     gene            20804..>21195
                     /locus_tag="VGM46_14565"
     CDS             20804..>21195
                     /locus_tag="VGM46_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010915023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HEY4193866.1"
                     /translation="MLKNAFLTLLALAIAIGGGGASLWYALKAQEGIGAIHVGVWTAF
                     PDIGTADADPYSKARVAREGVLALGRAEGLSFVAEKDSGGEPLKRQCSYTIEGGFAAA
                     RFWTLYAADGSLDAIATGKARPAALHSY"
BASE COUNT         3970 a         6940 c         6483 g         3802 t
ORIGIN      
        1 ccagcgcatg gcgcgcgagg accccgctgt cgccggctgt gacggcgcat tgtccagccg
       61 caggatacgc cgtgcgattt cagcgcgagc ctgatcggcc tgcgaggcgt ggatcatgcc
      121 gcgggcaaca tcacggtcga gttccgacaa ttgatcgcga taaacttcga ggtcgtgact
      181 ggagccttcg cccatgccct ttggtcgcgc ggccagcggc agcagcaccg ccaggctcgc
      241 gcccaatgtc aaaacagcgg ctatgaccca gaacagcatg tggtttccaa tagcggcaga
      301 gcggtcctgt gccaacatcg ccatcctgcg acgctttggc gaagtgctgg ccaacatatg
      361 ccggcggcgg tcacgatgtc gttatttcag gcctttaggg cctgcaacct aagtcagcgg
      421 cgtccagctg ccgtcggcat tgcgacaggc cgtgccgcgc gcgctggcgc tctggcctcc
      481 ggtaaagacc gtgtgggtat attgccggca gtcctgcgag cctacgcggt aaggctgcgc
      541 ggccacgacc tcgccatagc ggctggaact accgcttttc caggcgaccg tctggccgct
      601 ggcggtatat tcgagcgcct tatattcggc ttccagcccg ctgcgctttt cctggtcggt
      661 caatcccgca ccgatcttgc cgccgaccag cccgccatcc atcgccgaga taatcgcctt
      721 ggcgaccgtc gcgccaccgc tttgctggct ggacggcgcg ggggacagca cgccgcctcc
      781 gcctttgccg aggcccgtcg aggcgcaacc ggaaacaatc aaaagtccgg aaacgaatgc
      841 tgcgcgtatc ttcatctact gatccaggcc cggattgcac cgccatcgga aaccggcgct
      901 gcgataatat ctcaattggg ccggatttcg gccagttcat tcaaacgttc aaattcacga
      961 aaggctccgc aatcgaacga ccgctttaag gccgccgagg ctggagcgct ccaatgccag
     1021 cgtgcctcca tactcggtga cgagttcggc gacaatggca aggccaaggc cgctaccggg
     1081 cttcgtctcg tccaggcgcg ccccgcgctt caacgcctcg cgcgcctttt cctcgggaat
     1141 gccggggcca tcgtcctcga ttgcgatttc gaagcggtcg gcagcacttt cgtccggcag
     1201 ccgggccacg gaaaccagca ccgcgccctt tgcccatttc atcgcgtttt ccagcagatt
     1261 gccgatcaac tcctccaaat cctcacgctc gccggcgaat acgatatcct cgtcggccat
     1321 cgacagcgac aggccggctt gcgggttgag cttttgcacg acacgcacca tgcgccccat
     1381 cagcggcgcg accggcgtgc ggtagaccac gctgtcgcgc tgggccgcga cccgcgcgcg
     1441 ctgcaaataa tggtccacct gcttttgcat ggaagccgcc tgctcggcga taagctggcc
     1501 cttggcgccg cccagcgccc ggctttcgtt gatcagcacc gcaagcggcg tcttgagcga
     1561 atgggcgaga ttgccgacct gcgtgcgcga gcgctcgacg atccgccgat tgttttcgat
     1621 cagcgcattg gtctcgttgg cgagcggctc gatctcgacc ggaaatctgc ccgtgagcct
     1681 ttgggcggtt ccttcacgca ccatggccaa tgcgttgcct accctgcgca atggctgaag
     1741 cccaagcagg atggcgacgg cattgatgga aatcatgccg aaaccgaaca gcgacaggta
     1801 ggtggccagc cggcgctgga acgccgcgat ttcctgctcc agctcgctct ggttgcccat
     1861 gacgcgaaaa cgcgcggcgt gattcttgtt atcgagcgcg aattcgcttt cgaaaacctg
     1921 caattcctcg ccgtcgatgc cgtcggtcgt ataggtgcgc tggaaggccg tgttgaaggg
     1981 aacctcggct gcgctgggcg atggaatatc ctcggtcatc gacgaggaat gacgctcgcc
     2041 ggtaacgcct tcggaagccg gttgaacctg ccaataccag ccggattcgg gttctgagaa
     2101 acggaggtcg ccaagatcgg gcgagccggt cagcacggcc ttgtcggaaa tgccgaccga
     2161 accgatgaga ttgaacaaat gcgccgataa aaggctgtcg aacccccgtt cgctggcctg
     2221 ccggtaaagc gtcgtaatca gcgtgaagat gacgatcagc gcaaggatcg cccatacggt
     2281 cgaaaaggcc ataacgcgaa agcccagcga acgcggccac gaccgtatcg ctcgcggcaa
     2341 cgcaaaaaat cgcctggtgg gaagcggttc cgctttacgc ctccggctca cgcatgcggt
     2401 agcccatacc gcgcacggtt tcgatcatgt cgatgcccat cttcttgcgc agacggccga
     2461 cgaatacctc gatggtgttg gaatcgcggt cgaaatcctg gtcgtagaga tgctcgacca
     2521 gttcggtgcg cgacacgacc ttgcccatat ggtgcataag ataggcgagc aggcgaaatt
     2581 cgtgcgaggt cagcttcagc ggtacgcctg cgacatcggc cttcgacgcc ttggtatcga
     2641 ggcgaagcgg cccgcaggtc agctccgacg aggcatggcc ggcggcgcgg cggataagcg
     2701 cgcgaatccg cgccagcact tcctctatat ggaaaggctt ggccacataa tcatcggcgc
     2761 cggcatcgat gcccgaaacc ttgtcgctcc agcggtcgcg ggcggtgagg atcagaaccg
     2821 gcatctttcg accgttgcgc cgccaacgct cgacgacgct gatgccatcc atctgcggaa
     2881 ggccaatgtc gaggacaacg gcatcgtaag gctcggtgtc gccaaggaag tgcccctcct
     2941 cgccatcaaa ggcgcgatcg acgacatagc cggcatcgac cagggcgtcg gagacctgcc
     3001 tgtttagatc cttgtcatcc tcgacaacga gtacgcgcat gaaatccttg agttccgggc
     3061 gttgattttg gcaattccag atcagctata ggccgattcc ggcgtctttg gaaacgccgg
     3121 cgcccctcct caattctggg gaacgacgat ctcggaccgg cgcggccgct ggccctcctt
     3181 gccgggcacg agcacgacga tcacgcagac cggttgcccg ccctgcgtgg attgcgaggc
     3241 cttggcgagc gtcccgccat tctgctcggc gacctgctgg ccgatggcat aacaatccga
     3301 cgcgaccttg actacggcgc gggaatcgcc ttgcgcgacg gacagcccgg ccagcggcat
     3361 cgccgtcgcg tgcgacaaca gaagcccggc tgagcaaatc gtcaggaccg cccgtctgaa
     3421 aagggtgaaa aacgcattca tgtccagcct tctaacgcag cggtgctgaa cgtgatatga
     3481 acggtaacca ttcgtgatcc atgcgttgcc ctcccccgcc atgttggcgt ccgtcgagcc
     3541 gaggctcgca gacgccgaca ttatctagcc gatccggtct ttcgcggaca atcgtccaaa
     3601 aatggcgatc aagccggaag ccgcgctgat gatttgcagc agcgtatcgg taagcgcgcc
     3661 attatccaag ccatccaaag gaaggccggt aaggcccgcc gtgcccgtca ggacgctgac
     3721 gatggatgcc cagatcgtgc gcgaaagata ccagggtttg ctggttgtca tgtcttcatt
     3781 cctcttcttt gaagtgtcga tctttatcag gagacagcga gccggcgcgt cgccggcagt
     3841 ccccagccgg tgcggatgct gagttggcgc acggtcacgt cgatttccgc gggcggtgaa
     3901 gcgaagtccg tcgcgatatc ggctgcggga taggtccatt cggattcgga aacgatccgc
     3961 gtccttacgg tcgcgccgcc cggcgcggcg atatcgacct gatattcctc gcgttcctca
     4021 cccaacggga tttcggtgct ttcccattta tccgcgtcga tacggccccg gcgtatccag
     4081 gagagcttta tgtcgtcgcc gacccggcca gccttcagat gcacaggcga cagcggcagc
     4141 agcgatcgga tgccccccgt ctcgtttcgc gtcaaaaaat cggcactgga gaggctggag
     4201 ccggacggac cgacccgcca gttcaatgca agccccacct cgccatccag caatcccgcc
     4261 ggacgcaccg catcgtccag catgacgaag tcggagccga cagccgctcc ctcagccatt
     4321 gcgtcgtccg ttcccaactg tccgcgcaac agcccacgca actgccatat ctgcgacgat
     4381 acttcctcgg catccgagaa ctggatgatt tcccatgcac ctgaagcgga gcgtatcgcg
     4441 gcggcattgg cgccgttgag cagttgcagc atgctgatgc ttgttgcctg cgcgtcgaac
     4501 aactccacca atatctcgcc ttgccggtcg atccgcccgg taaaacccgg ttccagcgcc
     4561 gacgccagcc ggcccaggct tgcggccttg ccgaggcttg agcgaaaggc aaaacccgta
     4621 tcttcaggcg atgcaaacag cgactggctg cgccaaggct tctgccagga ggcggcgcgg
     4681 aactgggtca ccggatcgtc cgcgccgatg cctgccggca agtccagaaa aagcgcgtgc
     4741 ggctgtccgg tcaggatggg cgccggcgcg ttgccggaca gcatggatgg gcgtgaaggt
     4801 gcgggcgtgc cgcgcgcgac ctggcgggcg gaaactttgc gcaccaaccc atcctcgact
     4861 tgcaccacca ggaaatcgga cgaactgccg gaagccggca gccgcacgat agagcccggc
     4921 tcgacgtcga cgcgaggctg ggcaacggag aaggtgatgg tttcacgctc gtaccatacg
     4981 cgatccaacc aatcgtctag cagcgccttg ccttggccca cttccagcac gccgggaaaa
     5041 gagaggtttt gttggcgact gcccgccgcg ccaagccgcg tgccgcgcac cgatgccgac
     5101 tggtattcgg tcaaggcggt aaggaacgtc aaagtagctt cggcaggcag ttgatggtcc
     5161 ggcaagcgca cggtctcgat cagcgggcca tcctcgtccg caaccagttc ggtcagatag
     5221 aaggtcgggt ccggcgttgc ccccgcctgc ctgaaaacca gcgcatcggc ttcctcgcgc
     5281 acaatgatgt cgaacagatc ggtcaatggc tcgatggcgg atcgagccga ggtcgggtcc
     5341 gtgaccagat agccctcgac tgttccctcg acgtctgtgg tgtcggctgg cggctggcca
     5401 tgatccgcca ggatggcgtt gatcagcgca gccaggctcg gataagcaag cctgccgttc
     5461 agccagtggc cgcgatcaaa attggcgctg tccgaccaca ggtcgccgcg caacggaaag
     5521 gccggataag gcctggcgtc ccacgaccat ggatagatcc gcgtcgcatc gaccattcgc
     5581 ccgtcataga cggaagatcg cgggttgcgg gcttcctcga aatcaggctg ggcttgatcc
     5641 caatagtcca tgtgggcttg cagaaatgcc tgctgggcgg catcggaacg gcccccattc
     5701 gagaagtagg gcgaggcgtt ctccaccgat ttcggatctg gaaagacatt gggctggttc
     5761 ggccccttgt cggttgccgg gcatccgact tccgtgaacc agatcggctt gccttgcgcg
     5821 acccaggccg tcggcgtggc cacttccaca gcgcctatcc gatcatggtg cgggttggac
     5881 caccaattgg cgatatcctt gtaacggtaa acccacggct tgccgtaggc gccatcggtg
     5941 atcggcgacc gaacccgccc ttgcctcgct tcgctggagg catagtacca gtcgaaccct
     6001 tcgccgcccg caatcgaggc ggtcaggccg tgcggatcgt aagggccggc aaagccgtcc
     6061 ggattgccgc cggcatggtc gttgtcgcgc cagtcggaca gcggcatgta attgtcgatg
     6121 ccgaccgcat cgatggccgc atgtgcccac aaggcgtcga gatggaaata ggcgtcgccg
     6181 ctgccgtcct cgggatgatg gccgaaatac tcgctccagt cagctccata ggtaaggcgt
     6241 gtctgcgcac cgacgatgga cctgacatcc gccgccaggc cacaaagctg ttcgacgaac
     6301 ggaaacgccc cgtgctcgtc gcgcagcgtg gtcagaccgc gcaattcgct gccgatcagg
     6361 aacgcgttga cgccgcccgc tttcacggca agatgcgcgt aatgcaggat catgcggcgg
     6421 tagctccact caccgggggc accgataaag gccacggtgt cgtttgcctg ctcgaactgc
     6481 gatggctgcg cggtaccgca aaacgctgag acctgcgccc gcgccgccgc cgtcttgtcg
     6541 gcggagcctg gttgcatggg cgcgggatcg gcggttatgc ggccgcgcca gggatagctg
     6601 ggctgaaccg aaccgccata agggcttgga aaggcattgc cttcggcaac gtccataagg
     6661 atgaacggat agagcgtcac cgaaaggccc cgcgccttca attcggcgat cgcgctcagc
     6721 acgctggcat cggagggcgt gccgccaaac gcgccgccgc catcgtgaac ggatacgacc
     6781 ttggcctctt cccgcgccgt cgcggaaacc cgccattcgc tggacaggcc ctgttcgtcg
     6841 gcggtggtaa ccgctggttc tatacggcaa tggcccgccc gcagatcgtc gccaaaccac
     6901 gagacgacca gcgcgacatg ctggaggctg gggcacagca tctgcaattc atcgagtgac
     6961 gccgcgatat cggtgccggc aaacaggaca tgccggttga cggcttcctg gtcgcctggg
     7021 cggcgctcgc ggctgacgag ttccgccgaa aggccatatt cgattgcgcc tggaatgagc
     7081 gagatcgcct tgatcttccc gttcagccgg ccgacgggcc ggatgacctc gaattgcaat
     7141 tgtgggacgc ggttgccgaa ctcgccaata tcgaagcgtt cgagcacgac ataggcgacc
     7201 ccgcgatatg ccggcgtatt gccgctgccc tgcttggcct cgatcaacgg atcggccggc
     7261 tggtcggcgc tgccgcgata aacccgcatc tcgatcttct cgcgatcgat ctctcgccca
     7321 tcggcccaca cgcgccggat gccggcaatc tcgccctcgc aaagcaggaa ggcgacattg
     7381 gcgaaatagg aatattccgt aaccttggga ccaagcttgc cttgacgact ggtcgcgctg
     7441 ctttcctcga agcgcgtcgc ccagaccagg atgccgccga tgcgcgcggt tccataaaga
     7501 cgcgcgatcg agacgccctc ttcggcagtg aagggcctgg cgctggtcag gcgcggcccc
     7561 tcgatgtgct gggtgctgtt gatcagggcg cgatcgacga catagccggc gacagcgccg
     7621 gcagccgacc ctatcgcgcc gcccacggaa ccgagaaaac cgccgaggta agcaccggca
     7681 gcctgtagca gaattgtcgc catgaccgct ccggctctag gttgaaatgt ctttgaattc
     7741 gggaaaagaa aaggcggccg cgacgcgccg gcgccactgc ggcaccaggg cggaaaccat
     7801 taccgcgccc gccccctgat aggcatgaac aaagcggtgg ccctccacca gtataccggc
     7861 atgcttggcc ggcagattgg gccgccagcg aaatacaaac acatggccag ccttggcgtt
     7921 cgacggagca agctcgtgga aattacgccg catcccggcc agcaaatcct cgccgccgcc
     7981 ggcttcggcc caatccggcg cataggggcc gggcgcttca agatcgaacc cgtaaagcgc
     8041 gcgccagatc ccgcgcacca gccccaggca atcgcagcct acgcccttgc ggcatgcctg
     8101 gtgcagatag ggcgtttcca cccatgtcat cgcctcgcgg acaatttcag cggcaattgg
     8161 cgtatcgcgt ttaatttgcg gctctatcac gggacgaccg gcccgccatc gaaattcccg
     8221 ccatccacga cataggaata ggccgcgtcg ttgcccggca gatgcgggaa gccgcgaaag
     8281 ttcagcgcat tgtcgaactt cgccttgcag gtggcgaagt tcttgtcgca gccggccgcc
     8341 agggtaaaac cgtcgccggt ggcaattgcc ggacccacgc tttgctgaag gaccagaaac
     8401 gcatcgccgt tggtcaatcg atggtcgacg atcctttcgg tccgccctgc ccgcccgccg
     8461 gtagtccagc gcagcgtgcc gagagaaaac cagccgccgt cgaacccgcc cagtccggat
     8521 acggcgatct tgtccggcgc ctccagaact tggaccgcgc cttgcgcttg aaagcccggc
     8581 tggtcgagca ggaagccgca acgcgcgtcg cccaattccg cgtcgcagga acggctgacg
     8641 atgcggccat tgggctggtc gagccggctg accaggctct ccagttcggc gacgaaacgc
     8701 ccgtcgctgc gcgtcatctt gccgatagtc gccgtgcgcg tgagcacgaa gtcctgcggc
     8761 tttcgccaat tgaccaggaa ggtttccacc ttcgccccgt catagaggcc tgccgcaata
     8821 tcctcgtcgc ggatacgctg cgaagacagc gcgccctcca catcgaccgt atccacggcg
     8881 agacccagcg tgtctcgcgc ttcgctggcg ctgaagcccg aaccgggttc gaacaccgca
     8941 ccatcgaatg caagggcgaa atcgtggtcg gtatagccgg ttacatcgcc atcggttctg
     9001 gtcagacgcc agcaattgca gaccgtggtg acctcgcgcg acagatgatc gacaagttgc
     9061 tgcggatagg cgctcacaat tgcacctcga tcagcgggat cgacggtatc tgtccggcct
     9121 tgaaagcctt caggctgatt tccagccggt cggcattgaa gcggacgggc acgtcgaatt
     9181 cgtaaccgca ggtcacgccc tggccattgc cgggaatctt gcccgcggca aagacgactt
     9241 cgcccgtcgc atcgtcaaag ctgaacgcgc cagcttcggt aagttcgaca tcgtcgacgg
     9301 caacgcgcag gctcgaaatc accggctttg cgatcagccg ctggtaggcg tcgtcgcccg
     9361 ttccgtaggt ctttagcaac gcaaagcggg ccgtcacgcc atcaccggcg ccgagcggct
     9421 ggtcgtgggc ggcaggcgtc tgcgacggcc ggcacgactt cctgtcgaac ggatcgcgaa
     9481 agcggaaagc gtgcagggag ccacgccgcg cctcgaagaa cgccaccacc tcatgcaagt
     9541 catcgagcga cttgatgccc gtgccggcat cgtaatgatg gcgcgattgc gaaaaccgcg
     9601 cgttacgctt ttcgcggccg gaggtcaggg cgacgatttc gttgcggcgc tcggggccgc
     9661 cggtcgctcc aaaggagatg cccggcggga acagcacatc gtggaaggtg gaaagttcgg
     9721 acactgcata tccctcagaa ggttcgcgtt ccgcgcgata cggcgcgcgc cagcatgccg
     9781 gtgatctgcg cttcggattt gcggaatgag gcggcatcct gcgccgtcac gttgaaaacg
     9841 acattgaccg ccccgcctcc gccggcggag gcgacgccaa ggctcccgtc cggtcccctg
     9901 cgcagcggaa ggatcgcttc gctgccggcc tcgcccatca ggcctatatt gccgccgagc
     9961 ggaaaatagc ttggcgagga gacgacgccg cccgatgcga acggcacgac gctgcccggc
    10021 acgccgccct tggcgaacgg caagataccc gacagcccgc cgagcaatcc ggaaaacagc
    10081 gatcccgtca gcgattgcag cggcttcaat ccttgcgtca gcgccatgcc ggcaaggttc
    10141 agcccgacgc ggcgaagcac atcgtccagt tcacggccgc tgacggcggc gcttttcagc
    10201 gctccgctaa gctggctgcc gaaattgccg gagagccttt cgaggctttc cagcgcattc
    10261 tggaaaggtt cggtgtcggc gcgtatggcc acagtcacat cttcagccaa atccagtctc
    10321 catcgctgta gggtcgggga acgcccgcat caacgcggca aggtcggcgc ggccgggcgg
    10381 cgaagccctg gccggtcgca ccgcgctcat cgcgcgctcg aattcaatcg gggtcattgc
    10441 ccagaacgcc cccggcgaaa gccgcagcag gccgaagccg atggccatga cgtcgtccca
    10501 gggaaacgcc gccttcgcgc ctgccacggc ggctaagggt ttgggccggt ctcgtcgttc
    10561 gccgccctgc cgaaggtcgt gccaagcagg tccgccacga tttcggcaaa gcctgccgcg
    10621 ccgccctcgg attgcatctt gccgacggca tcgtcggtca cttgctcgcc tgcgccgcgc
    10681 aggcccgcgc caacgatgcg gatcatgtcg cgcgccgaca gccgcccggt ggagaaacgc
    10741 tccaccagcg ctgcaagatc gtctgccgca taggccgcct ccagttcggc cagcgcgccc
    10801 aacgtcaggc aaagccggtg cggtgcaccg tccagttcgg cggaaatctc gccgcgcctg
    10861 cggttagcgc tcacgacgcc accgcgaatt cgatcgaacc cgccgattcc agcgccattt
    10921 cgaatgtcac ctcgccatca tgcgcgccgg tatattccag cgaagtgatc tgaaacggcc
    10981 ccgccaccgt gccgaaaccg ggcacagcca attgccagtc cgaaatctcg ccggcgaaga
    11041 agcgcgcccg gatgtcggcg tccgactgcg cgtccttgaa gatgcctgaa ccgctgatgg
    11101 cggcgcgctg gacgcccgcc cccgccagca attcgcgcca gcgcccggcg gagtccgaat
    11161 cggtgatatc gaccgtctcg ctattgaagg cgatgcgctt ggagcgcagg ccggcaaccg
    11221 tgacgaaatt gccgatccca tcggaatcga gcttgagtag aaggtccttg cccttctgtg
    11281 cgaccattgt tggcctcctg ataatggggg tgttgcggtg ggcttaagcg atccgcctca
    11341 agcggattgt tcgatcacgg cgcgaaagcg cagcaggccg tgatgaaccg actggtcgtc
    11401 gtcgtaacgg gactcggcat attcgaagcg aaggctgacg agatggtgct gatcgagcgc
    11461 caggctgcca tcgtcgagcc gcgcccggac ggcctccatg atatcgagcg tttctttctt
    11521 gcccttggcc ttcgaccaga tgtggagcgt aaaaagctgc tcggtgccac tgtcggtatc
    11581 ggtgctccag tcgtagacgc tggtgcggcc gaaagtaatg tacggaaagc gcacgtcagc
    11641 cggcgcgtgg tcgaatatct ttgcgccgtc aagcagcgcc gccagcgcat tgtcgcccgc
    11701 cagcgcctcg aacagggttt tctggaggtc agcgacgggt gaaatcatct tcttgcctcc
    11761 tctcatgtcc ctgcatttgc ggtgaaagct tttcgtcttc cggttcgccg gcggccgaag
    11821 cgtaaccgcc ttccagcgcg tcggccagat cgtgcgcggt gcggcgcaag gcctggataa
    11881 gcccatccat ggtcagcgtc atggccaact tcatgcgccc gcctcgcgaa ccttgcagac
    11941 cagataacgg ccgctttcgt ccggatcgtg aacggtaacg atgtcgaaaa cacggtctac
    12001 cctggtgaag cgcatgccgc tcgccacgcc gccgcgctgg cgcagcgtga tgcgatgcgt
    12061 caccgtctca agcgtctggt cggccccgaa cacgctttcg gccgacagcg gctctatctt
    12121 cgcaaacagc gttgcggctt gcgtccagat ttcggtgaag ccgccaagtc cgtcggcttc
    12181 ggtcgtgcat gcttccagcg caagccgcgc gcggaattca ccgggatcga tgaacaaaac
    12241 cggcatcaca gcctccttgc gcggaagggc gcgatcatgc gctcatagac ctgcgggtag
    12301 gaaaccggct ggtcgtccgg gccgaacgcc gtacggaact cgtaccagtg caggacgagc
    12361 agcgcgatcg cccgcttcag gagatccggc acatcgacgc cggcctcccc gtagccggcg
    12421 acaaaatcga tctcgatgcc gttgagcgcg cgcagggtgc gcggcgatgc ctcgaaatgg
    12481 agccgggccg ggcgcgaaac cgtgtcggcc agatatgtcg ccgggtcgat caggcaagcc
    12541 tcgccctcgg cgccataggc tgtgacggaa agtatctcgc gcaccgggta aaccgggatc
    12601 gtcgcacaac cgttacgcgg ccatcggtcg agcacgagac gccagttctg gtcgatcaac
    12661 gccagaccgg tcgagcgttc gacatcctcg cgcgcggcac ggatgaggcc gccgatcaag
    12721 tcgtcgtcgg tgtcgtgatc cagccgcata ttcagcttca tctcggcaag cgtcaccggt
    12781 tcggcggttg ggccgacggt tcggatgagc gtcattcaat caccttggat ttgagaaagg
    12841 agccgcgtgc accttggatt tgagaaaaga acggctccgg ccaaatggcc ggagccgtct
    12901 gccggcaggc ataaaggcga gcgacggagg ggcgagcggg aggcttaccc gccccggatc
    12961 atgccgtgcc gaacttcagg agcttgatcg cgtcgaagtc ctggacgccg ccgccaacgc
    13021 gcttggtggt gtagaagagc acatagggct tggcggaata cgggtcgcgc agcacgcgca
    13081 cgccggcccg gtcgacaacc aggtagcccc ggctgaaatc gccgaaagcc agcggcgtcg
    13141 catctacgcc gatgtcgggc atatcctcgg cttcgaccag cggaaatccc atcagcgcgg
    13201 cgcgctggcc ggcgacggat ggcggctgcc agaggtagtt gccgtccgcg tccttcagct
    13261 tgcggatcgc cgcctgcgtc ctgcggttca tcacccagtt ggcattctgg cggtagccag
    13321 ccttcagcgc atagatggtg tcgatcagga tatccgacgg gctgtcggcg gacatggcgc
    13381 cggaaacacc ggtcagcgtg tagccgatct tgccccacgc ccagcttgcc tcagcaaccg
    13441 tcgcatagct cagaaagcct ttcggcttgc tggcgccgtc gcccgaaatg aatgccgctc
    13501 cttcctgttc ggcaaaggcc gcctcgacct cggaagaaat ccattgatcg agatcgacca
    13561 ccgtgtcctc aagcagcgat gcggtcgcag ccggcatggc gtaaagctcc atggtcggga
    13621 actgcaactc ggccagcgtc gccgtatccg tctgcggccg cgatgccgtc tcggctaccc
    13681 agcctaccgc cggaccgctg accgaaaacg gcttcttcag caccgcgctg gaaacctgcc
    13741 tgaccgacgc gatagagcgg atcggcgaca gcaccgacag ccgcttgccg atttccacct
    13801 cggtttcggc cggcaccaga taaccgccat cctggccgga gccataggac atcgccttgg
    13861 tatcgagagc gcgaagctga cggtcatcgc cgctgcgcat ataggcgtcg aaagcgccct
    13921 tgtgctccga cgaaacgacg gcgccatcac ggccgagcgt cggcctgatc ttcttcaagg
    13981 cgagattgtc gatggcgcgc ttctgctcgt cgagcgcgtt ggagatacgc tcgaccttgt
    14041 ccacggtcac cgtgtcggcg cccatgcggc cctccagttg ggcgagcttc tgatcgttcg
    14101 attccttgaa ggcctcgaac gtgttcatga aatcgccaaa ggcgtccttc aggtcgaaat
    14161 attcgccgga cgccgatttg gtctcgatcg acgcaggctg atgcgttgca ttcatagggt
    14221 atttcctctt tggttgatga gattggtggc ttcgcgaatg cgcgccgcga ggccttcgac
    14281 ggttcccggc gcggcttccc gctctcgcac cagattggcg aaacccctgg cgataacgcc
    14341 acgggcatcg ctgcgtgaca gccccgcatc ccgcgtgagc cacaactcga actcccggac
    14401 ggtcggcagg cgtttcgcgc gcctgccttt gaccgtctgg atgcgggcat cgggcagcat
    14461 tgggaacgtg acgaccgaaa tttcccaaag gtccgcttcc agaatatggc gaacgccgct
    14521 tgccggatcg cgcctggcct tgaccgcccg aaagccgatc gacagcccgt ccagcgcgcc
    14581 gccgcgcatc aggctcagca cttcgcgggc gcgccgcaca tcctttgcca ggcggccgcg
    14641 gacgaacagg ccgcgcgcgt cttccttgat ttcggtccag acaccgatcg gctcgccggg
    14701 atcgtgctgg aaaagcatgc ggatgccggc tgcgccccga acgcgcagcg actttgcaaa
    14761 tgcgcctctt tcgacggcgt ccttgccgag atcgaccttg ccgaacaggc tggcatagcc
    14821 ggcgaaactg ccgtcggcct cgacctcgtc gagaaccaga tcgacgaact tgcgttcgca
    14881 agcccgctcc aacgcctcac ttctcatgcg tatcctccga agttttcttg ccaggcaggt
    14941 cattttcgcg aagcaggtcc ggcctgcgaa acaggtcgtt cttgcgaaac aggtcgggca
    15001 ggctgctttg ctggagcgcg cgcaccacga agcccagcgc ccaccatgcg cacaggctgg
    15061 cggcggccga gcccatcagc atcagttcca ccgggccgat gacgttttcg acgctcaatt
    15121 cgctcgatat tttcaggccg gcggtgccgc cgaacaccag gccgcaaacg acgccgacgg
    15181 caaagcgcga cgccgcctcg cgacgtccgt gcggcaggat atagaccagg gaaatcgccg
    15241 agccggcaac ggcgccggca cccttggcga tccataccca ggccggttgg gaggggtcgg
    15301 tcatggcgca cctcttttct ggagggatca aacgaggtcg cgcggccgat agccgaccgc
    15361 ctcgcgcttc tcgtcttcgg taaggaaagt ggccgcatcg atgcgcgccc acagcgcgcc
    15421 gcggtcggca ttcaggcttt cgatgcggtc ggcgtcgtac cagaggcgca acgagccgcc
    15481 gaaagccgcg ccgagccagc cggacaattc cttcgccgtg cgcgccacca gcggcaacac
    15541 cgtcagccga tagaacgcgc gattggcttc ctggtagttg gcaaaggtat tgtcgcccgg
    15601 aatgccaagc agcatcggcg gcacgccgaa ggcaagcgcg atgtcgcggc tggccgcgtg
    15661 cttggcctcg acgaaatcca tgtccttggg cgtcatgccc atggccttcc agtcgagacc
    15721 accttccaga agcaacggtc gcccggcgcg ggtggcgcct gaatagccgt cttccagctc
    15781 gcttttcagc cgggcgaact gttcatcgct gagattgccg ccatccttcg gcgcatagac
    15841 aagcgcgccg gagggccggg ccgaattatc gagcagcgcc ttgttccaac gcccggcgga
    15901 attgtgggta tcgagcgcca tgagagccgc ttcgagcggc gggaaaccgt aatggtcatc
    15961 cagcggatgg aacaatgtca ggtgcagcgc cccgccgccc tcgccggcac caagtggcac
    16021 ggttcgccgc tcgctgcccg cgcgatagtc gagcgccgtc ggccaaccgc ttgcgtccgt
    16081 caccaccgac acgcgatcgg ggcgcagaag gtgcatttcg cgcgcgccgt tgccggtgtc
    16141 gatcagttcg acataggcgt ttccggcgag cagcagatga ccgtaaagcg catccagaaa
    16201 cgttgcgccg gcctgacgct ggttgggacg ttccagcaag ttcagcagcg ggtgatcgtc
    16261 tatctcgacc acgccgtcat aaagcagcca gggcacggcg gacgccgttt ccgagatcaa
    16321 ccgcaccgaa cggtgcacga tcgggttgcg catgaacccc tcgcggcaaa gcgtggcgta
    16381 gtcgcggcgc gtccagcgcg cctccccttg cgcatgaaag gcgatgaagc ctccggcgga
    16441 cgcgctcttg ttttcaggac gcacgccggc attccccgca gcgcgaggcc aagaccagtt
    16501 ccaagccata tttttccctt atatgaaatc gcgtattcgc gggtgcgccg accaggccgg
    16561 catcaattcc gtgaccgccc agaccagcgc gtcgacacga tcgggcgagc ggccgttgga
    16621 caggccgttg gggccgaaat cgcacatttc gtcttccagt tcgccgaacc gcccggcatg
    16681 gcggaccctg ccctgctggt aaagtgctgc tatcggttca gcgcgcagcc actttccgcg
    16741 ctgcgcgcgg accgccttga ccggaacggt cggatcaacg gtgcggataa ccgccgttgc
    16801 catctcgccg ccctggttga cttcgacaac caggcaatcc gcctgcaatt tatgaaacag
    16861 ctcgaccgcc tgccttgccc attcctgcgg cttggcggtg ggcaaggtgg cgtcggccag
    16921 cacgatcacc tgaccctggc catcaagccc cgccgccacg atgccgcaag cgtccgaggt
    16981 ttttcgcgaa ctggcgggcg ggtctaccgc gaccacgatc cgccctagcg gaccggcggc
    17041 ggccagcgcc acctcctcga tcatcgagcg cgaccataag gcgtcctcgc ggtcctcgat
    17101 catctcgccg cccagttcct gacgcccaag gcgcgtaccg gcatagcggc gctcgaccat
    17161 cttcatgaaa cccggcgcca ggttggcggc gttttcatcg gtgcgcatgc gcgtcaccac
    17221 gaccgatgga tcggcaagca agcgcttcat gagctttgtc gggcgcggcg tggtggtgat
    17281 gatctggcgc ggctgcgcgc caaggcgcag gccaaactgc aacatgtcga agcaagcctc
    17341 ggcatagcgc cacttcgccg cctcatcgca ccaggccgcc tcgaactgat ggccgcgaag
    17401 gctttctgga tcttccgaag aaaaaatggt ggcgaccgcc ccattgtccc acaaaaggcg
    17461 gcggcggcta gcctcgaagc gcggtcgatc gttgcgcgaa atggtgagga tgccggaagg
    17521 gccttcgatc atcacgtcac gcacatcggc cagcgtttcg ccgatcagcg caaaacgccc
    17581 atgccggcgg tcggcaaagg gggaaaaccc acgaaccaag ccattgaccc actcggctcc
    17641 cagccgcgtc ttgccggccc cgcgcccgcc catgaccagc cagctatccg gctttgaccc
    17701 cagcggatat tgcgcgtccc tggcgatttc cagccattct cccctgatga gggccagctc
    17761 agccagcgtc agatttttct ccgaccagcc gcctcgcata gccaacggca agctcgataa
    17821 tgcgctcgtc aattcgtttg aggacgtcgg cgatgtttgc atctctcctc gcttggttct
    17881 ctttcgcgcc ggtatcgctg cgcgtgattt cgccaatttt ctcgagtgtt ctcatcaccg
    17941 cggaaattgc atccatgcgg gctttgtcgg ccgaggcgct tgcatccgta tcgttgccgg
    18001 aaatctccgc cttggttatc tgctggtcga gcaggacgcg aagccgcatt tcctggctgg
    18061 aaagctgcat cgccatttcc gcgtcgcgcc aaccttccct ttcggctcgc ttgcgcagcg
    18121 cggccgaaga ccggccggcg gcggttgcca acaggtcgag gctggccgcc tcgccctcgt
    18181 gaagagcgcg caacgccgcc cattgcaaag caggcttcgc aaccatctgg ttccatccat
    18241 tcattgaatt gctgggattt ggaattgtcg ggatttgccg gaaagcgaaa tcgcttgggc
    18301 ggccctggcc gcacatttcc gacgatgacc aaacactacc agagcaccgt tacggtgtca
    18361 agaataaatt cctaattatt ttgacgccgg gcgctttcga gcggcaatct ttgtttgcgc
    18421 ttgtgggcgc ctgcattatc agtgagaaaa cggcatcgcg tgttatgctg aaattgcttt
    18481 tgaccgcctc tgatttcacg tagaagccgc gtccggattt gcccatggtc gaccccgctg
    18541 agaagcagaa gaaaagaggc gcgcttgccg ccttcctgct ttcgctcgac gcgcgcctcg
    18601 attccgccct ctatgaaacc ggcttcaagg cgcgggaatt ctgggaagcg gcaacgattt
    18661 tctttcggcg ctttcgcgtc accggctggc ggcgcggcat cgtcgaaatt ctcagcgaag
    18721 ccttcacgat cggcgccggc ggctcggtca tcatgctggc gctcgccttg ccggcatttc
    18781 aagacaccac cggcgactgg cgcgcgcaag gcgatttcgc cgttaccttc ctcgaccgct
    18841 acggcaacga tatcggccag cgcggcatta tccagcgcga cagcgtgccg gtcgacgaga
    18901 tgcccgatca tgtcatcaag gcggttctcg ccaccgaaga ccgccgcttc ttcgagcatt
    18961 acggcatcga ttttctgggt ctggcgcgcg ccatgtcgga aaatgtgcgc gccaattcgg
    19021 tcgttcaggg cggctccagc atcacgcagc agctggcaaa aaaccttttt ctctccaacg
    19081 agcggacgct ggaacgcaag atcaaggagg ctttcctgtc ggtctggctg gagtgcaacc
    19141 tgtcgaaaaa ggaaatcctg caactctatc tcgaccgctc ctacatgggt ggcggcacgt
    19201 tcggcatcga ggcggcggcg gatttctatt tcggcaagag cgtcaaggat ttgaaccttg
    19261 ccgaggcggc gatgcttgcc ggcctgttca aggcgccgac caaatatgcg ccgcatgtca
    19321 accttccggc ggcgcgggcg cgagccaatg tcgttttgtc caatctcgtc gatgccggct
    19381 tcatgacgga aggccaggtg ctgtcggcgc ggctgcatcc ggccgacgtg gtcgatcgcg
    19441 gcgagcagaa aagccccgac tattacctcg actgggcgtt cgacgaggtc aagaagatcg
    19501 ccgccaaatc cggccagcac tcgcttgtcg cgcacaccac cttcgacgcc aaccttcaga
    19561 aggcggcaga ggaatcgctc gaattccacc tgcgccagtt cggcaaggaa tatcgcgtcg
    19621 cggaaggcgc aatcgtcgtc atcgagacga acggcgcggt gcgcgccatt gtcggcgggc
    19681 gcgactatgg cgccagccag ttcaaccgcg ccaccaaggc gctgcgccag accggctcct
    19741 ccttcaagcc ctatgtctac gcaaccgcca tggagcatgg cttcacgccg gattcggtga
    19801 tctccggcgg gccgatcagc tggggcggat ggtcgccgca taattatggc ggcgcggccg
    19861 ccggcaaggt cacgctgacc accgcaatcg tcaagtcgat caacaccgtg ccggtccggc
    19921 tcgccaagga ctatctgggc atcgcgccga tcaaggcgat ggccctgtcg ttcggggtgg
    19981 aatcgccgct cgaaagccat aagaccatgg ttctcggcac ctccggcatg acggtcatgg
    20041 accaagccac cggctatagc gtgtttgcgc aaggcggcat ggtgggcaca aggcatggca
    20101 tcacccagct tgtcacccac accgggcagg tcgtctacga ctatgccaag gatgctccgc
    20161 cgccgcgccg ggtgctatcc gaaaaggcgc tgaaagccat gaacaccatg ctgtcgcagg
    20221 ttccggtgat cggcacggcg cgccgcgcgg cgctgcctgg catcgtcgtt gccggaaaga
    20281 ccggcaccac ccaatcatac cgcgacgcct ggttcgtcgg cttcaccggc aactacaccg
    20341 ccgccgtctg gctcggcaac gacgatttca gcacgaccaa caacatgacc ggcggcacgc
    20401 tgccggcgat gatctggcag cgcctgatgg cttacgcgca ccagaacatc gacctcaagc
    20461 ccattcccgg cctggaacat ccgttcatcg atgctgaggt tgcggccaaa gccggggaag
    20521 ccgaaaagaa aaaggccgag caggaagcag ccgataccgc tgcctcaagg ccgcccgtat
    20581 tgtccagcgg caccacgcgc accctgcggg aaatgtcgca tctcttccag tcggctccca
    20641 atctcggcaa ttcggccaat ccggcagagc caaccaattc agccaatcca gccgggccag
    20701 ccaagccgga gacgctttca gcattatagc tctcaccgag gcctggcttg ccagaacacc
    20761 ctgcccccga tatccccaac ggtaattctc gttcaggcgg cccatgctga aaaatgcatt
    20821 cctgacattg cttgcgcttg ccatagccat cggcggcggg ggcgccagcc tgtggtatgc
    20881 gctcaaggcg caggaaggca tcggcgcgat ccatgtcggt gtctggaccg cttttcccga
    20941 tatcggcacg gccgacgccg atccctattc gaaagcgcgg gtggcgcggg aaggcgtgct
    21001 ggcgctgggg cgggcggaag gcctttcctt cgtcgcagag aaggattccg gcggcgagcc
    21061 gcttaaaagg caatgttcct atacgatcga aggcgggttt gcggcggcgc gcttctggac
    21121 gctctacgcc gctgatgggt cgctcgacgc tatcgccacg ggcaaggcgc gtccagcggc
    21181 gcttcattcc tacca
//
LOCUS       DASYCS010000467         2439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_432371,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000467 DASYCS010000000
VERSION     DASYCS010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432371"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..222)
                     /locus_tag="VGM46_14570"
     tRNA            complement(133..222)
                     /locus_tag="VGM46_14570"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(186..188),aa:Ser,seq:cga)
     gene            750..1367
                     /gene="rplU"
                     /locus_tag="VGM46_14575"
     CDS             750..1367
                     /gene="rplU"
                     /locus_tag="VGM46_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEY4193867.1"
                     /translation="MFAVIKTGGKQYRVAANDLLKIEKLEAQVGDIVEIGTVLAHGEG
                     DNVIFGAPFVAGALVTAEVVEQGKNRTVIAFKKRRRQNSRRKIGHRQLLTTVRISEIL
                     TDGAKPSKKAAAKPAKAAAKDETAPAAAAAPLFRAPKGEPDNLVDIKGIGPVAAEQLK
                     EQGIITFAQLAKLSDADVAKIDEHMPFSADQIKDWREQAKELTKK"
     gene            1469..1738
                     /gene="rpmA"
                     /locus_tag="VGM46_14580"
     CDS             1469..1738
                     /gene="rpmA"
                     /locus_tag="VGM46_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004676172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEY4193868.1"
                     /translation="MAHKKAGGSSRNGRDSHSKRLGVKKFGGEAVIPGNIIIRQRGTT
                     WHPGVNVGMGTDHTLFALEAGAVTFNKKANGRTYVSVNPMLKAAE"
     gene            1858..>2439
                     /locus_tag="VGM46_14585"
     CDS             1858..>2439
                     /locus_tag="VGM46_14585"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018430098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY4193869.1"
                     /translation="MVAESEDIEDECLAMDCPVIATESLVLRAPRESDLDQLAQLADN
                     RHIAEMLARMPHPYGEAQARAFLAMTKTRRNGAAYALTIAGTDIFVGCAGLNTTDRGL
                     ELGYWIGEPYWKRGYATEAAHALVDLAFRSTRIQLLQASCRVINPASRRVIHKCGFQY
                     AGQGMLNSIVAGQVPVERYRLDRKTWTSLRNWAH"
BASE COUNT          515 a          778 c          729 g          417 t
ORIGIN      
        1 ggccgcttta tcgactctgc cgttgctaca cctagtaggc ggtaggaaaa taatcctaat
       61 gtgggtagaa tcgatggtcg aaaccgcgtc gttccgttaa ccaattggtt ctattgcttt
      121 atttggaata aatggcggag agggagggat tcgaaccccc gatgggcttg cacccatgcc
      181 gcatttcgag tgcggtgcat tcgaccactc tgccacctct ccgcggtcat ggtcggccgt
      241 tgccggcgcg gcgcactaga taacggctga acgggcaggt tacaaggcca atccgtcagt
      301 tatttggctt gccacgcaag cagcggaatc atcgccgcgt tcgcgagcta cggcgctggc
      361 ggatcatagg cagccttggc gatcatcgcg gcggtcacgg acctctccag ggcgctttcg
      421 gcgaccggtt cggcgccgac agcgcgggct gccatgccag cagcgccaaa accaggcaga
      481 gccccgaccg gcccgcatcg tctttccccc ggatgcaaac gcccaggcat gttgacacaa
      541 ccaccccgag ccgaccaata acggtctcgg caacggctgt tcgccttgac tcaggcgcaa
      601 ccttcggtta gggacagccc gcaatcggcg tggagggttc tccgcgccgc ttgttttttg
      661 aacccgcaga ctgacaaaaa gacggccgaa ccgccctctt cccgaggata ccggttcatc
      721 gaggccgacc gaacagcgaa aggcaaaaaa tgttcgcagt cattaaaacg ggcggcaagc
      781 agtatcgcgt cgccgccaac gatctcctga agatcgagaa gctcgaagcc caggtcggcg
      841 atatcgtcga aatcggcacc gtcctcgccc atggcgaagg cgacaatgtt attttcggcg
      901 caccgttcgt cgccggcgcg ctggtcacgg cggaagtcgt cgagcagggc aagaaccgca
      961 ccgtcatcgc tttcaagaag cgccgccggc agaattcgcg ccgcaagatc ggccaccgcc
     1021 agttgctgac caccgtccgc atctcggaaa tcctgacgga tggcgccaag ccttcgaaga
     1081 aagccgctgc caagccggca aaggccgccg cgaaggatga gaccgctccg gctgccgccg
     1141 cagcgccgct gttcagggcc ccgaagggcg agccggacaa tctcgtcgac atcaagggca
     1201 tcggcccggt tgccgccgag cagctcaagg agcagggcat catcactttc gcccagctcg
     1261 ccaagctttc ggacgccgat gtggccaaga tcgacgagca catgcctttc agcgccgacc
     1321 agatcaagga ctggcgcgaa caggccaagg aattgacgaa gaagtaagct gctggcccat
     1381 tattcggggc cgacagcgca cttgaaacgg acgccaagcg cgttcataag gattcgaggc
     1441 gtcctcgcgg cgcaaaggag ttagacaaat ggcacacaag aaagctggcg gttcgtcgcg
     1501 caacggccgc gattcccatt ccaagcgcct tggcgtgaag aagttcggcg gcgaagccgt
     1561 cattcccggc aacatcatta tccgtcaacg cggcaccacc tggcatcccg gcgtcaatgt
     1621 cggcatgggc acggaccata cgcttttcgc gctcgaagcc ggcgcggtca cctttaataa
     1681 aaaagccaac ggccgaacct acgtatcggt gaacccgatg ctcaaggccg cggagtagcc
     1741 ggttccgtac aagaacaccg gcgcccatct cgggaaccgg tgtctggcca agccaggaaa
     1801 tggatcaggg gagatgggca agccatctcc cctttttctt tgcctggagg aattcaaatg
     1861 gttgccgagt cggaagatat cgaagacgaa tgcctggcga tggactgccc ggtcatcgca
     1921 accgagagcc ttgtcttgcg cgcgccgcgt gaaagcgacc tcgatcagtt ggcgcaactg
     1981 gccgacaacc gccacatcgc cgaaatgctg gcccgcatgc cgcatcccta tggcgaggcg
     2041 caagcccgcg ccttcctcgc catgaccaag acgcggcgca acggcgcggc ctatgcgctc
     2101 acgattgccg gaaccgatat tttcgtcggc tgcgcggggc tgaacaccac cgatcgcggg
     2161 ctggaactcg gctactggat cggcgagccc tattggaagc gcggttatgc gacggaagcc
     2221 gcgcatgcgc tcgtcgatct ggcgtttcgc agcacgcgca tccagctgtt gcaggcttcg
     2281 tgccgggtca tcaacccggc ctcgcgccgc gtcatccata aatgcggctt ccagtatgcc
     2341 ggccagggca tgctgaactc catcgtcgcc ggccaggtgc cggtcgagcg ctaccggctc
     2401 gaccgcaaga catggaccag cctgcgcaac tgggcgcac
//
LOCUS       DASYCS010000468         2758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_289725,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000468 DASYCS010000000
VERSION     DASYCS010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289725"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2112)
                     /locus_tag="VGM46_14590"
     CDS             complement(<1..2112)
                     /locus_tag="VGM46_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019565871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY4193870.1"
                     /translation="MNKVTDILKSMEYGPAPEDSAIVGDWLARHEGGFGHFIGGAFTE
                     PGETFEVSNPATSKVVAHVTQGSDADVEAAVKAARAAQPKWAALSGHERAKYLYALAR
                     HIQQHSRFLAVLETIDNGKPIRESRDIDIPLVVRHFYHHAGWAEILADEFSGMAPYGV
                     CGQIIPWNFPLLMLAWKVAPALAAGNTVVLKPAEYTPLTALALAEIASEIGLPKGVLN
                     IVTGDGATGAAIVRHAGIDKIAFTGSTEVGREIRRQTAGSGKSLTLELGGKSPFIVFA
                     DADLDAAVEGVVDSIWFNQGQVCCAGSRILVAEAVAARFEELLRRRMDTLVTGDPLDK
                     STDIGAIAHPVQLQRIKALLERGRKQGATLYQGSAPEGCFYPPTLATDVEPASVLAME
                     EIFGPVVTLTAFRTPEDAVALANNTKYGLAASVWSENVNLALDIAARVKAGVVWINSV
                     NVFDAGAGFGGYRESGFGREGGREGMAAYLRHPEISARKPEQPSAALVTTPAAGLQDA
                     AGIDRTAKLYIGGKQARPDSGYSYGVAGAKGPVGLAGLGNRKDIRNAVEAARKASSWG
                     AQTGHNRAQVLYYVAENLSARAAEFEARLRSFGQSAQAARNEVEMAIRRIFWYAAQAD
                     KFDGAVHQTKTANITLAMNEPFGVIGIACPAALPLLGFVSLVLPAIAMGNRVVAVPSQ
                     AHPLAATDLYQVFDTSDVPGGV"
     gene            complement(2126..>2758)
                     /gene="deoC"
                     /locus_tag="VGM46_14595"
     CDS             complement(2126..>2758)
                     /gene="deoC"
                     /locus_tag="VGM46_14595"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010660794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEY4193871.1"
                     /translation="PHAVKALRGTRIPVASVATGFPAGLLPLKLRLEEIRYAVGEGAD
                     EIDIVITREHVMTASWTALYDEISAMREACGDAHLKAILATGDLQTLRNVYSASMVAM
                     QAGADFIKTSTGKEGVNATLPVSLVMVRALRDYRDATGHRIGLKPAGGMKSAKDALNW
                     QFLMKEELGRDWLEPALFRLGASSMLGDIERQLEHYVTGRYADASRHALA"
BASE COUNT          461 a          963 c          863 g          471 t
ORIGIN      
        1 gacgccgccc ggcacgtccg acgtgtcgaa cacctggtag agatcggtcg ccgccagcgg
       61 gtgcgcctgc gaagggacag ccaccacgcg gttgcccatg gcgatcgccg gcaacaccag
      121 cgaaacgaag cccagaagcg gcagggccgc cgggcaggca atgccgatga cgccgaacgg
      181 ctcgttcatg gcgagcgtga tattggcggt cttggtctga tgcaccgcac cgtcgaactt
      241 gtcggcctgc gcggcgtacc agaaaatgcg ccggatcgcc atctcgacct cgttgcgggc
      301 agcctgcgcg ctctggccga agctgcgcag ccgcgcctcg aattcggcgg cgcgggcgga
      361 aaggttttcc gccacataat agagaacctg cgcgcggttg tgccccgtct gcgcgcccca
      421 gctgcttgcc ttgcgcgccg cctcgacggc gttgcgaatg tccttgcggt tgcccagtcc
      481 ggcaaggccg accgggcctt tcgcgccagc gaccccatag gaatagccgc tgtcgggccg
      541 cgcctgcttg ccgcctatgt aaagcttggc ggtccgatcg atgccggcgg cgtcttgcag
      601 tccggctgcc ggcgtcgtca ccagcgctgc ggaaggctgc tccggtttgc gcgcggaaat
      661 ttccggatgc ctcagatagg cggccatgcc ctcgcgcccg ccttcgcggc caaagccgct
      721 ctcgcggtat ccgccaaagc ccgcgcccgc atcgaagacg ttgactgaat tgatccacac
      781 cacgcccgcc ttgaccctgg cggcgatgtc gagggcgagg ttgacgttct ccgaccagac
      841 cgaggcggca aggccgtatt tcgtgttgtt ggccagcgcc accgcatcct ccggcgtgcg
      901 gaaggcggtg agggtgacca ccggcccgaa tatttcctcc atggccagca ccgatgccgg
      961 ctccacatcc gtcgccagtg tcggcggata aaagcagcct tccggcgcgc tgccctgata
     1021 aagcgtcgcg ccctgcttcc tgccccgttc gagcaacgcc ttgatgcgct gcaactgcac
     1081 cggatgggcg atggcgccga tatccgtcga cttgtcgagc ggatcgcccg tcaccagcgt
     1141 gtccatgcgc cggcgcagca gttcctcgaa acgcgccgcc accgcctcgg ccaccaggat
     1201 gcgcgagccg gcgcaacaga cctgtccctg gttgaaccag atggaatcga ccacgccttc
     1261 gacggcggcg tcgaggtcgg cgtcggcaaa gacgatgaac ggcgacttgc cgccaagctc
     1321 cagcgtcagc gacttgccgg aaccggcggt ctggcggcgg atttcgcggc cgacctcggt
     1381 cgagccggta aaggcaatct tgtcgatacc cgcgtgccgc acgatggccg cgccggtcgc
     1441 gccatcgccg gtgacgatgt tcaacacccc cttcggcagg ccgatctcgc tggcgatctc
     1501 ggccagcgcc agcgcggtaa gcggcgtata ttcggcgggc ttcagcacca ccgtgttgcc
     1561 cgccgccaaa gccggcgcca ccttccacgc cagcatcagc agcggaaaat tccacgggat
     1621 gatctgcccg cagacgccgt acggcgccat gccagaaaat tcgtccgcca gtatctcggc
     1681 ccaaccggcg tggtgataaa agtgacggac caccagcgga atgtcgatat cgcggctttc
     1741 gcggatcggc ttgccgttgt cgatcgtttc cagcaccgcc aaaaagcgcg aatgctgctg
     1801 gatgtggcgg gcgagcgcat aaagatattt cgcccgctca tggccggaaa gtgccgccca
     1861 tttcggctgc gccgcgcgcg ccgccttcac cgccgcctcg acgtccgcat cgctgccttg
     1921 cgtcacatgg gcgacgacct tgctggtagc cggattggaa acctcgaatg tctcacccgg
     1981 ctcggtgaac gccccgccga tgaaatggcc gaagccgccc tcatgccgtg ccagccagtc
     2041 gccaacgatg gcgctgtctt ccggggcggg tccgtattcc atgctcttca gaatgtccgt
     2101 gaccttgttc atgccgcgct cccgatcatg ccagcgcgtg ccgcgaggca tccgcatagc
     2161 gcccggtgac ataatgttcg agctggcgct cgatatcgcc cagcatcgac gacgccccga
     2221 gccggaaaag cgccggctcc agccagtcgc ggcccagttc ctccttcatc aggaattgcc
     2281 agttgagcgc atccttggcg ctcttcatgc cgcccgccgg cttcaggccg atgcgatgcc
     2341 cggtcgcgtc gcggtaatcg cgcaacgcac gcaccatgac gaggctgacc ggcagcgtcg
     2401 cattgacacc ctccttgcct gtcgaggttt ttatgaaatc ggcccccgcc tgcatcgcca
     2461 ccatcgaggc ggagtagacg ttgcgcagcg tctgcaaatc gcccgtcgcc aggatcgcct
     2521 tgaggtgggc gtcgccgcag gcttcgcgca tggcggaaat ctcgtcgtaa agcgccgtcc
     2581 agctggccgt catgacgtgt tcgcgggtga tgacgatatc gatctcgtcc gcgccttcgc
     2641 caacggcata acggatttcc tccaaccgca gcttgagcgg cagcaggccc gccggaaagc
     2701 cggtcgccac cgaggcgacg gggatgcgcg tgccgcgcag cgccttcacc gcatgcgg
//
LOCUS       DASYCS010000469         6465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_1157676,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000469 DASYCS010000000
VERSION     DASYCS010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 6465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6465
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1157676"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1319)
                     /gene="aspS"
                     /locus_tag="VGM46_14600"
     CDS             complement(<1..1319)
                     /gene="aspS"
                     /locus_tag="VGM46_14600"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006332500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEY4193872.1"
                     /translation="MTMHRYRSHTCAQLRKQDVGSSVRLSGWVHRVRDHGGLLFIDLR
                     DHYGLTQIVADPDSPAFKIAETLRGEWVVRVDGEVKARLADTVNANLPTGEIEIFARE
                     IEVLSASKELPLPVFGEPDYPEDIRLKYRFLDLRRDTLHRNIVQRTKIIAEMRRRMES
                     AGFTEFSTPILTASSPEGARDFLVPSRIHPGTFYALPQAPQQYKQLIMVSGFDRYFQI
                     APCFRDEDPRADRLPGEFYQLDLEMSFVEQDDVLATMEPVMRGVFETFADGKPVTQKF
                     QRIPYDVAMRKYGSDKPDLRNPIEMQAVSDHFRDSGFKVFANILANDAKAEVWGIPAK
                     TGGSRAFCDRMNSWAQGEGQPGLGYIFWRKEGEKIEGAGPLAKNIGEERTEAIRQQLG
                     LGEGDAAFFVAGDPKKFVAFAGAARTRAGEELNLVDRDRFELCWIID"
     gene            1514..2665
                     /gene="rnd"
                     /locus_tag="VGM46_14605"
     CDS             1514..2665
                     /gene="rnd"
                     /locus_tag="VGM46_14605"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HEY4193873.1"
                     /translation="MQVITTQKELETALAAFEKSDFVTVDTEFIRETTFWPILCLIQM
                     AAPGVTALIDPLSPDIDLKPFFDLMANEKIVKVFHAARQDIEIIVHLGDLVPHPVFDT
                     QIAAMVCGFGDSVSYDQLVQKITRTQLDKSSRFTDWRHRPLSEKQLDYALADVTHLID
                     VYQYLTAQLERENRAHWLNEEMVVLTSRETYDPKPEDAWKRLKMRVRKPQELAIVQAV
                     ATWRELEARERDVPRGRVLKDDAIYEIAQQAPRDAAALARLRTIPKGWERSSTAAALL
                     EAINTALALPKEALPRVPKAFQPPEGSNAAAEILKVLLRIIAEKESVAAKVLASSDDI
                     DRIAAEGEAANVPALQGWRREVFGEAALRMVRGEVGIKFDKRRIEVFDL"
     gene            complement(2700..3119)
                     /locus_tag="VGM46_14610"
     CDS             complement(2700..3119)
                     /locus_tag="VGM46_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006332485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEY4193874.1"
                     /translation="MNPTAIFWPMVTHVALVCAIYVLLGYRRRQVLVSDTARPSVFKT
                     RASEPEASASVAANLMNQFELPMLFHVVCLALFVTNGVSFIAVTLAWIFVLLRYVHAL
                     IHVTSNDLRYRSPAFAAGFIVLVILWIWFALHLTGAV"
     gene            complement(3144..4646)
                     /gene="guaB"
                     /locus_tag="VGM46_14615"
     CDS             complement(3144..4646)
                     /gene="guaB"
                     /locus_tag="VGM46_14615"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006332478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEY4193875.1"
                     /translation="MAKIIETSTGALALTFDDVLLQPGHSEVMPGETDIRTRIARDID
                     LNVPILSAAMDTVTESRLAIAMAQAGGIGVIHRNFSPVEQAEQVRQVKKFESGMVVNP
                     VTIGPDATLADALGLMRTYSISGIPVVENGGAGGHKSGRLVGILTNRDVRFASDPSQK
                     VYELMTRENLITVKENVDQDEAKRLLHQHRIEKLVVVDKQGNCVGLITVKDIEKSQLN
                     PNASKDAQGRLRAAAATSVGDDGFERAERLIDAGVDLLVIDTAHGHSQRVLDAVTRAK
                     KLSNSVRILAGNVATADGTQALIDAGADAVKVGIGPGSICTTRIVAGVGVPQLSAIMS
                     AVETAQKSGVSVIADGGIKFSGDLAKALAAGAGAAMIGSLLAGTDESPGEVYLHQGRS
                     FKAYRGMGSVGAMARGSADRYFQAEVRDTLKLVPEGIEGQVPYKGPVSGVLHQLAGGL
                     KAAMGYVGGRDLADFRERATFVRISNAGLRESHTHDVAITRESPNYPGGM"
     gene            complement(4970..5794)
                     /locus_tag="VGM46_14620"
     CDS             complement(4970..5794)
                     /locus_tag="VGM46_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PopZ family protein"
                     /protein_id="HEY4193876.1"
                     /translation="MATASSVQREPSMEEILASIRRIIEDSDPGRKQPETGDELKQDL
                     EPDMVEKPEAEVEAFRAELRGSLETRKPFTLADVQAKMAAEQSPAASDPVDNSQQPVR
                     SPLTLAEVGTQAGVKTSDSDGPALKTAYSADTIVADWRRDIANAAQPVQAAPAAEVRE
                     EPVFDLASDIDIYEDELTPEAPPLAAAQPTFTDSSPADEEPALRPAIVSEHTGRQVAA
                     AFGELSEAFASRSKKSFDDMAQEMLRPMLQDWLDNNLPTLVERLVREEIERVARGA"
     gene            complement(6090..>6465)
                     /locus_tag="VGM46_14625"
     CDS             complement(6090..>6465)
                     /locus_tag="VGM46_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006335248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY4193877.1"
                     /translation="VSRDNVRAAVTSAWTQYTASREGVDANRALVAAAQLALNGVIEE
                     RNVGQRTTLDVLNSQNDLITAKINLVSSERDVVVASYAILSAIGHLSTERLGLQVTKY
                     RPEEHYNAVKDKWYGLRTPDGR"
BASE COUNT         1251 a         1965 c         2038 g         1211 t
ORIGIN      
        1 aagtcgatga tccagcacag ttcgaaacgg tcgcggtcga cgaggttcaa ttcctcgccg
       61 gcgcgggtgc gggcggcacc tgcgaacgcg acgaatttct tggggtcgcc ggcgacgaag
      121 aaggcggcat cgccctcgcc caggcccaat tgctggcgga tcgcttcggt acgctcttcg
      181 ccgatgttct ttgccaacgg cccggcacct tcgatcttct cgccttcctt gcgccagaag
      241 atatagccaa ggcccggctg tccttcgcct tgcgcccacg aattcatgcg gtcgcaaaag
      301 gcgcggctgc cgccggtctt cgccggaatg ccccacacct cggccttggc gtcgttggcc
      361 aggatattgg cgaacacctt gaagcctgaa tcgcggaaat ggtccgatac ggcctgcatc
      421 tcgatggggt tgcgcaggtc gggcttgtcg gagccgtatt tgcgcatggc aacgtcatag
      481 gggatgcgct ggaatttctg cgtcaccggc ttgccgtcgg caaacgtctc gaacacgccg
      541 cgcatcaccg gctccatggt ggccagcacg tcatcctgct cgacaaagct catttcgagg
      601 tcgagctggt agaattcgcc cggcaggcgg tcggcgcgcg ggtcttcgtc gcggaagcac
      661 ggcgcgatct ggaaataacg gtcgaagccg gaaaccatga tcagctgctt gtactgctgc
      721 ggcgcctgcg gcaacgcata gaacgtgccg ggatggatgc gcgacggcac caggaaatcg
      781 cgcgcgcctt ccggcgacga ggcggtcagg atcggcgttg aaaactcggt aaagcccgcg
      841 ctttccatgc ggcgacgcat ctcggcgatg atcttcgtgc gctgcacgat gttgcggtgc
      901 aaggtatcgc ggcgcagatc gaggaaacgg tacttcaggc ggatgtcttc cgggtagtcc
      961 ggctcgccga acaccggcag cggcaattcc ttcgatgccg acagcacttc gatctcgcgc
     1021 gcgaaaatct cgatctcgcc ggtcggcagg ttggcgttca cggtgtcggc aagccgggcc
     1081 ttgacctcgc cgtccacgcg aaccacccac tcgccgcgca gcgtctcggc gatcttgaag
     1141 gcgggtgaat cgggatcggc aacgatctgc gtcagcccgt aatgatcgcg aaggtcgatg
     1201 aacagcagcc cgccatgatc gcgcacgcga tgcacccagc cggaaagacg aacggacgag
     1261 ccgacatcct gcttcctcaa ttgagcgcag gtatggctac ggtagcgatg catggtcatg
     1321 gaagaaatcc gggtgttgcg ttgcgagcca ccgcctagcg gcgcggcctt gaaattggcg
     1381 cgaaaagcgc atgggaggcc ctgtttgtca aggtaatggg gcgttatggc cctgaggatc
     1441 gccacgaagc ttgacgaaag catcgccggc tgaaatggaa agaaacatac tccgtttcga
     1501 aaaaagcgat tttatgcagg tcattaccac ccaaaaagag cttgaaaccg ctcttgccgc
     1561 gttcgaaaag tccgatttcg tcacggtcga tacggaattc atccgtgaaa ctaccttctg
     1621 gccgatcctg tgcctgatcc agatggcggc gcccggcgtc accgcgctca tcgatccgct
     1681 ttcacccgac atcgacctga agccgttctt cgatctgatg gcgaacgaaa agatcgtcaa
     1741 ggttttccat gccgccaggc aggatatcga aatcatcgtg catctcggcg acctcgtgcc
     1801 gcatcccgtg ttcgatacgc aaatagcggc gatggtctgc ggttttggcg acagcgtttc
     1861 ctacgaccag ctggtgcaga aaatcaccag gacgcagctc gacaaatcat cccgcttcac
     1921 cgactggcgc catcggcccc tgtccgagaa gcaactggac tatgcgctgg ccgacgtcac
     1981 ccatctgatc gacgtctatc agtatcttac ggcccagctg gagcgggaaa accgcgctca
     2041 ttggctgaac gaggaaatgg tcgtgctgac ctcgcgcgag acctatgacc cgaagcccga
     2101 agatgcctgg aagcggctga agatgcgcgt gcgcaagccg caggaactcg ccatcgtgca
     2161 ggcggtggcc acatggcgcg agcttgaggc ccgcgagcgc gacgtgccgc gcgggcgcgt
     2221 gctcaaggac gacgccatct acgagattgc ccagcaggcg ccgcgcgacg cggccgcact
     2281 ggcccgcttg cgcaccatcc ccaaaggctg ggagcgttct tccaccgcgg cggcgctgct
     2341 ggaggcgatc aacaccgcgc tggccctgcc caaggaggcg ctgccacgcg tgcccaaggc
     2401 tttccagccg ccggaaggct ccaatgcggc agcggaaatc ctcaaggtgc tgttgcgcat
     2461 cattgcggaa aaggaaagcg tcgcggccaa ggtgctggcg agcagcgacg acatagaccg
     2521 tatcgctgcc gaaggcgaag ccgccaatgt tccggccctg caaggctggc ggcgcgaggt
     2581 gtttggcgaa gcggcgctgc ggatggtgcg cggtgaagtc ggcatcaagt tcgacaagcg
     2641 ccggatcgag gttttcgacc tttaaaaagg cggctacgcg ttggtgaaat tgcgctgcgt
     2701 cagaccgcgc cggtcaggtg caatgcaaac catatccaca ggatgaccag tacgatgaag
     2761 ccggcggcga aggccgggct gcggtagcgc agatcgttgc tggtgacgtg gattagcgca
     2821 tgcacatagc gcaacagcac gaatatccag gcgagcgtga cggcgatgaa cgaaacgccg
     2881 tttgtcacga acagcgccag gcacacgaca tgaaacagca tcggcagttc gaactggttc
     2941 atcaggttgg cggcgaccga ggcgctggct tccggttcgc tggcgcgggt cttgaacacg
     3001 cttggccttg cagtatcgct gaccaggacc tgtcggcgac gataacccaa cagcacgtag
     3061 atcgcgcaga ccaaagccac atgcgtgacc atcggccaga aaatggcggt cgggttcatc
     3121 ggtagctcct taggccggcc tgattacatc ccgccgggat agttagggct ttcgcgggtg
     3181 atggccacgt catgcgtgtg gctttcgcgc aaaccggcgt tggaaatgcg aacaaaggtg
     3241 gcgcgctcgc ggaaatcggc aagatcgcgg ccgccgacat aacccatcgc cgccttcagg
     3301 ccgccggcaa gctgatgcag cacgccggag acgggtccct tgtagggcac ctgcccctcg
     3361 atgccttccg gcaccagttt cagcgtatcg cggacctccg cctggaagta gcggtcggcc
     3421 gagccgcgag ccatcgcgcc taccgaaccc atgccgcgat aggctttgaa ggaacggccc
     3481 tggtgcaggt agacctcgcc cgggctttca tcggttcccg ccaagagaga gccgatcatg
     3541 gccgcgccgg cgcccgcggc aagcgccttg gcgagatcgc cggaaaactt gatgccgcca
     3601 tcggcgatga ccgagacgcc cgatttctgc gccgtttcga ccgccgacat gattgccgac
     3661 agctgcggca cgccgacgcc ggcgacgatg cgcgtggtgc agatcgaacc cggaccgatg
     3721 ccgaccttga cggcatcggc gccggcatcg atgagcgcct gcgtgccatc tgcggtggcg
     3781 acgttgccgg ccaggatgcg cacggaattg gagagttttt tcgcccgcgt gacggcgtcg
     3841 agcacgcgct gcgaatggcc gtgggcggtg tcgatgacaa ggaggtcgac gccggcatca
     3901 atcaggcgct cggcgcgctc gaaaccatcg tcgccgacac tggtggcggc cgcggcgcgc
     3961 aagcgccctt gcgcatcctt gctggcgttg ggattgagct gggacttctc gatgtccttg
     4021 acggtaatca agccgacgca attgccctgc ttgtcgacga cgaccagctt ctcgatgcgg
     4081 tgctggtgga gcaggcgctt ggcctcgtcc tgatcgacgt tctccttgac cgtgatgagg
     4141 ttttcacggg tcatcagctc atagaccttt tgcgacgggt ccgaggcaaa gcgcacgtcg
     4201 cggttcgtca ggatgccgac cagccggccc gacttgtgac cgccggcgcc gccattctcg
     4261 accacgggga tgcccgagat gctgtaggtt cgcatcaggc caagcgcgtc ggccagcgtg
     4321 gcgtcgggac cgatggtgac ggggttgacg accatgcccg attcgaactt cttgacctgg
     4381 cgaacctgct cggcctgctc gacgggagaa aaattgcggt ggatgacgcc gatgccgcca
     4441 gcctgcgcca tcgcgatggc cagccgcgat tccgtcaccg tatccatggc ggcggacaga
     4501 atgggaacgt tgaggtcgat atcgcgggcg atgcgggtgc gaatgtcggt ctcgccgggc
     4561 atcacttccg aatgaccggg ctgcaaaagc acgtcgtcaa aggtaagcgc cagcgcgccg
     4621 gtggacgttt cgatgatttt tgccatggcc agccccaata agcgaagcgc ggcgcaactc
     4681 atatgggcag cgccgactcg tcattattcc ctttcaggat tggcggtggc tggtaacact
     4741 tctttgagcc gatgaaaagt gacgacgggc aactcaacgg caaagaacag aaaaaagtcc
     4801 gcagatattg ccgccggtga atttctggac gcttaaggca agccttgccc aaggcatgtt
     4861 acggcattga aaaccgaagc tgatccggtt tcaatgcggt gcggacgaat ggcctggcca
     4921 tcggcgttcg tgctgcactg gcgcacgtca tcccaactcg cgccggagtt caggcgccgc
     4981 gggcaacccg ttcgatttcc tcgcgaacca gccgctcaac gagggtcggc aaattgttgt
     5041 ccagccaatc ctgaagcatg ggccgcagca tttcctgcgc catatcgtcg aaggatttct
     5101 tgctgcggct ggcgaatgcc tccgaaagct caccgaacgc ggcggcaacc tgccggccgg
     5161 tatgttccga aacgatggcg ggccgaaggg ccggttcctc gtccgccggg ctggaatccg
     5221 tgaaggtggg ctgtgcggcc gccagcggcg gcgcttcggg tgtcagttca tcctcataga
     5281 tgtcgatatc ggacgccaga tcgaaaactg gctcctcccg aacttcagcc gcaggcgcgg
     5341 cttgcactgg ctgagccgca ttggcgatat cgcgacgcca gtcggcaacg atcgtatctg
     5401 cactgtaggc tgtcttgagg gcaggcccgt cgctatcgct cgttttgaca cccgcctgcg
     5461 tgcccacttc ggccagcgtc aggggcgaac ggaccggctg ttgcgaattg tccaccggat
     5521 ctgacgcggc cggggattgc tccgccgcca tcttggcctg cacgtccgcc agcgtgaacg
     5581 gtttcctggt ttccagcgag ccgcgaagct cggcacggaa ggcttcgacc tccgcttccg
     5641 gcttttcgac catgtcaggt tcgagatcct gcttcagttc gtcgccagtt tccggctgct
     5701 tgcggcccgg atcactgtct tcgatgatcc tgcggatgga agccagtatc tcttccatcg
     5761 aaggttcgcg ctgaacgctg cttgccgttg ccattgtcat atccgccgcc ttgtggccgc
     5821 taaaagtttg tttccggccg aaaagccgaa acgaatcgca caagtgagcg tatcccactg
     5881 attgccatcc gagaatcccc attgtgggac gatgccggat ttcaacagat tcaatggatg
     5941 cgcatcggat ttgcgaacgt gggatttgga tggcgggtcg gacaggggtg aaaagcacca
     6001 ccgcgccgcg cccaagcagc ccgcttatga cagaagcgcc cgacaagggc gcctctgttc
     6061 aaattaggaa aaatgactac agggctggtt cagcggccat ccggcgtgcg caggccatac
     6121 cacttgtcct tgacggcgtt gtaatgctct tcaggacgat acttcgtcac ctggaggcca
     6181 agacgttcgg tcgacaggtg gccgatggcc gaaagaattg catagctcgc caccacgacg
     6241 tcgcgttccg aactgacgag attgatcttg gcggtaatca agtcgttctg ggaattcaac
     6301 acgtcgagcg tggtgcgctg gccgacattg cgttcctcaa tcacgccatt gagtgcgagt
     6361 tgcgccgcgg cgaccagcgc gcggttggcg tcgacccctt cgcgcgaggc ggtgtattgc
     6421 gtccatgccg aagtcacggc ggcgcgaacg ttgtcgcggc tgaca
//
LOCUS       DASYCS010000470         9149 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mesorhizobium sp. isolate SMAG_U4939 k141_2753817,
            whole genome shotgun sequence.
ACCESSION   DASYCS010000470 DASYCS010000000
VERSION     DASYCS010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958213
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesorhizobium sp. (soil metagenome)
  ORGANISM  Mesorhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 9149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,935
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,885
            CDSs (with protein)               :: 2,885
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9149
                     /organism="Mesorhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2753817"
                     /isolate="SMAG_U4939"
                     /isolation_source="soil"
                     /db_xref="taxon:1871066"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..955)
                     /gene="nuoF"
                     /locus_tag="VGM46_14630"
     CDS             complement(<1..955)
                     /gene="nuoF"
                     /locus_tag="VGM46_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEY4193878.1"
                     /translation="MLQDRDRIFNNIYGRFDRSLAGAMARGHWDNTPGIVAKGREWIV
                     NEMKASGLRGRGGAGFPTGLKWSFMPKQSDGRPSYLVINADESEPGTCKDRDILRHDP
                     HTLVEGALIAGFAMGAIAAYIYVRGEFVREREALQRAIDEAYAAKLIGKNNTSGYDFE
                     IYMHHGAGAYICGEETALLESLEGKKGQPRLKPPFPANVGLYGCPTTVNNVESIAVAP
                     TILRRGAAWFSSFGRPNNVGTKLFCVSGHVNNPCTVEEAMSIPFRELIETHCGGIRGG
                     WDNLLAVIPGGASVPLVPAEQIIDTPMDFDALRDLRSGLGTA"
     gene            complement(966..2228)
                     /locus_tag="VGM46_14635"
     CDS             complement(966..2228)
                     /locus_tag="VGM46_14635"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013894811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit E"
                     /protein_id="HEY4193879.1"
                     /translation="MSVRRLAEASVQPASFAFNKANTAAVKQWIKKYPKGREQSAIIP
                     LLMIAQEQEGWVTKAAIESICKMLDMPFIRGLEVATFYTQYQLAPVGTRAHIQVCGTT
                     PCMLRGSSELMDVCRSKIHHDQFHTNDNGTLSWEEVECLGACVNAPMVMVFKDTYEDL
                     TPERLAEIIDAFEAGKGETVKPGPQNGRVTSEPITGLTTLSDENAILKTTREKEARAA
                     AKAAQVPPSNAAKPATDKEETNPTLKAPVKTTPKPAPAAETASPSPAFDTKQANKAEP
                     SVEAVTGKQRVSASPRKAAPEGAYQSPEMLKGEPIGAGGKKVATAAARPSLEDKNRPA
                     GIDKPAAVDDLKLISGVGPKNETILHSLGIYTFAQVASWKKAERDWVDGYLNFHGRVE
                     REDWVKQAKALAKGGVAEYIRVFGKKPV"
     gene            complement(2229..3419)
                     /locus_tag="VGM46_14640"
     CDS             complement(2229..3419)
                     /locus_tag="VGM46_14640"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011424925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEY4193880.1"
                     /translation="MAETSVRNFNINFGPQHPAAHGVLRLVLELDGELVTRVDPHIGL
                     LHRGTEKLIEHKTYLQAVPYLDRLDYVAPMNQEHAFALAAERLLGIEVPKRGQLIRVL
                     YCEIGRIMSHILNVTTQALDIGALTPPLWGFVEREKLMVFYERASGSRMHAAYFRPGG
                     VHQDLPKQLIEDIGRWIDPFLKSLDDLDALLTPNRIFKQRNVDIGVVSLADAWAWGFS
                     GVMVRGSGAAWDLRKSQPYECYDEMEFDVPIGKNGDCYDRYLIRMEEMRQSARIMRQC
                     VDRLLGTESTGPVSNLDGKVVPPKRAAMKRSMEALIHHFKLYTEGYKVPAGEVYAAVE
                     APKGEFGVYLVSDGTNKPYRCKLRAPGFAHLQAMDFLCSGHMLADVTAILGSLDIVFG
                     EVDR"
     gene            complement(3428..4033)
                     /locus_tag="VGM46_14645"
     CDS             complement(3428..4033)
                     /locus_tag="VGM46_14645"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010661173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEY4193881.1"
                     /translation="MSEQLDDLAGHIAATVDAEILESVVAFGELTLTVKAQDIVGVAT
                     FLRDDPKCRFISIIDICGADYPSRAMRFEVVYHFLSPQQNTRIRVKVQADEDTLVPSI
                     TGVYIGADWFERETYDLYGVLFTGHPDLRRILTDYGFEGHPLRKDFPLTGFVELRYDD
                     EAKRVIYEPVELKQEFRNFDFLSPWEGTDYVLPGDEKAKAN"
     gene            complement(4038..4619)
                     /locus_tag="VGM46_14650"
     CDS             complement(4038..4619)
                     /locus_tag="VGM46_14650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010661172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEY4193882.1"
                     /translation="MGLSDSSGTLVAPRPKGILDPSTGKPVGADDPFFLGINDELADK
                     GFLVTSTEALITWARSGSLMYMTFGLACCAVEMIHSAMPRYDSERFGIAPRASPRQSD
                     VMIVAGTLTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRIVPV
                     DIYVPGCPPSAEALMYGLLLLQKKVRRTGTIER"
     gene            complement(4610..4975)
                     /locus_tag="VGM46_14655"
     CDS             complement(4610..4975)
                     /locus_tag="VGM46_14655"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009450025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEY4193883.1"
                     /translation="MNALISSYLPIVLFIGVALVVGVALLAAPFLVAYRSPDPEKLSA
                     YECGFNSFDDARMKFDIRFYLVSILFIIFDLEVAFLFPWAVSFSKIGMLGFWSMMVFL
                     AVLTIGFAYEWKKGALEWD"
     gene            5292..6518
                     /locus_tag="VGM46_14660"
     CDS             5292..6518
                     /locus_tag="VGM46_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEY4193884.1"
                     /translation="MREKISLGLARRIALAAQGFADPRPSVTPDRRHLARVLSRTGLL
                     QIDSVSAVVRAHYMPLYSRLGPYPLSLLDNAAVSRKRALFEYWAHEASYLPVETYPLM
                     RWRMERAQRGEEMYLGLAKWGHERQPLIEDIYNQVVAGGPIPASSIEGHKGSGGWWGW
                     SEAKNAFEWLFWAGRITTAYRRGFERFYDLPERVLPRHILDLPTPSPEDARRQLLRIS
                     ARAHGIATASSLRDYFRLSPADMKDRLEELVEEGEISPVRVEGWDKPAYLHKSAKMPR
                     RIETRALLAPFDPLVFERSRTEQLFGFRYRIEIYTPADKRQYGYYVLPFLLNERIVAR
                     VDLKADRPAGILRVHAAYAEAGAPAETAAELFEELKQMQAWLGLETIEVTPAGDLGPA
                     LADIALPHRRVDTAAN"
     regulatory      6520..6719
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6742..7170
                     /locus_tag="VGM46_14665"
     CDS             6742..7170
                     /locus_tag="VGM46_14665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006335685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1636 family protein"
                     /protein_id="HEY4193885.1"
                     /translation="MERDVNDDSYPTVSAEISTAVDATVIVCSSCRDETGSDVYPRAG
                     EKLADDARRAAMGENIHVRTVECLGNCKRRLSAAILRDGCWSYVFGDLTPDSGADLIA
                     GAKLFAGSADGLIPWRGRPDCLKRGLVARIPPLSLLKDRP"
     gene            7167..8204
                     /gene="cobW"
                     /locus_tag="VGM46_14670"
     CDS             7167..8204
                     /gene="cobW"
                     /locus_tag="VGM46_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CobW"
                     /protein_id="HEY4193886.1"
                     /translation="MTASVTRVPCTVITGFLGSGKTTLVRHLLENSKGKRLALIVNEF
                     GDIGVDGEILKGCGIDTCPEENIVELANGCICCTVADDFVPALDQILSLTPKVDHILI
                     ETSGLALPKPLVQAFQWPTVKSRVTVDGVIAVVDGPALAAGQVASDMDALAAQRAGDD
                     SLDHDDPVEELFEDQVACADLIILSKKDLMDEDGVARANAIIGKSRARAVQTVSVSHG
                     QVDPSVLLGLGLAVEDDIENRWSHHDGMGSHEHDDFDSFIVDVPSIANPDELAARVAD
                     AAEQENVLRVKGFVEVAGKPMRLLVQAVGPRVNHYYDRAWTPADDRRSRLVVIGLKGL
                     NRPAIEKILAG"
     gene            8224..>9149
                     /locus_tag="VGM46_14675"
     CDS             8224..>9149
                     /locus_tag="VGM46_14675"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020809834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HEY4193887.1"
                     /translation="MHILTTTSASLDDLAEPVDLRQTPADVVALSFTDSDLAGLAAAW
                     QADADSMPSMRLAPLRDLRHPMSVDLWIESVAQHAKVILVRILGGYDWWRYGCDRLSA
                     VARSRGIKLALLPGECREEDLRLIEASTLPRAELDGLLDYFREGGPANMSALVHHLAR
                     LAGRDTTVAEPVSVPKAGYYEPGVGVVETPAMYTGPVVAILFYRSMLLAADVAPIDAL
                     TEALGRRGIAALPIFVSSLKDPASLAFVENALACLHPSAIVTATAFASGAEPGSQTLF
                     DRASVPVFQVIVATTRRDIWQENQRGLAPSDLA"
BASE COUNT         1720 a         2924 c         2745 g         1760 t
ORIGIN      
        1 ccgccgtgcc gaggcctgac ctcaggtcgc gcagcgcatc gaaatccatc ggcgtgtcga
       61 tgatctgttc ggccggcacc agcggcaccg atgcgccgcc cggaatgacg gccagcagat
      121 tgtcccaccc gccgcgaatg ccgccgcaat gcgtctcgat cagctcgcgg aacgggatcg
      181 acatcgcttc ttcgacagtg cacggattgt tgacgtgacc ggaaacgcag aaaagcttgg
      241 tgccgacatt gttcgggcgg ccaaatgacg aaaaccacgc cgcgccacgc cgcaggatag
      301 tcggcgcgac agcgatcgat tcgacattgt tcaccgtcgt cgggcagcca tagaggccga
      361 cattggccgg gaacggcggc ttcagccggg gctggccctt tttgccttcg aggctttcca
      421 gcagcgccgt ttcctcgccg caaatataag cgccggcgcc gtgatgcatg taaatctcga
      481 agtcgtaccc cgaggtgttg ttcttgccga tcaacttggc cgcataggcc tcgtcgatgg
      541 cgcgctgcaa cgcctcgcgc tcgcgcacga actcgccgcg cacatagatg taggcggcga
      601 tcgcgcccat ggcgaaaccg gcaatcagcg ccccttcgac cagcgtgtgc gggtcatggc
      661 gcaggatatc gcggtccttg caggtgccag gctcggattc gtcggcattg atgacgagat
      721 agctggggcg tccgtcgctc tgcttgggca taaacgacca ttttaacccg gtcggaaagc
      781 ccgcgccgcc gcgcccgcgc aggccggaag ccttcatctc gttaacgatc cactcgcgcc
      841 ccttggcgac gatgccgggc gtgttgtccc aatgaccacg cgccatcgca ccggccagcg
      901 aacggtcgaa gcggccatat atgttgttga agatgcggtc tctatcctga agcattttgt
      961 tatcctcaga ccggcttctt gccgaagacg cggatatatt cggctacgcc gcctttggcg
     1021 agcgccttgg cttgcttgac ccaatcctcg cgctcgacgc ggccatggaa attgagatag
     1081 ccgtcgaccc aatcgcgttc ggccttcttc cacgaagcga cctgcgcgaa ggtatagatg
     1141 cccagcgaat ggaggatggt ttcgtttttc ggcccgacgc cggaaatcag cttgagatca
     1201 tccaccgcgg ccggtttgtc gataccggct ggacggttct tgtcttccag cgaaggccgc
     1261 gcggctgcgg ttgcaacctt cttgccgccg gccccgatcg gctcgccctt gagcatttcg
     1321 ggcgactggt aagcgccttc gggggcggcc ttacgcggcg aagcactgac gcgctgcttg
     1381 cccgtcaccg cctcgacgct cggctccgcc ttgttggctt gcttggtgtc gaacgccggc
     1441 gacggcgatg ccgtttcagc ggccggcgcc ggcttcggcg tggttttgac cggcgctttc
     1501 aacgtcggat tggtttcttc cttgtcggta gcgggcttcg cggcgttcga cggcggaacc
     1561 tgcgccgcct tggcggcggc cctggcttcc ttttcgcgcg tcgtcttcag gatcgcgttc
     1621 tcatccgaaa gcgtcgtcag gccggtgatc ggctccgaag ttacccgacc gttctgcggg
     1681 ccgggcttga cggtttcgcc cttgccggct tcgaacgcat cgatgatttc ggccaggcgc
     1741 tccggcgtca aatcttcgta cgtatccttg aacaccatca ccatcggcgc gttgacgcag
     1801 gcgccgagac actcgacctc ttcccacgaa agcgtgccgt tgtcgttggt gtggaactgg
     1861 tcgtgatgga ttttcgagcg gcacacatcc atcaattcgg acgaaccgcg cagcatgcag
     1921 ggcgtggtcc cgcagacctg gatatgggcg cgcgtgccga caggggccaa ttggtactgc
     1981 gtatagaagg tcgcaacctc cagtccgcga atgaagggca tgtccaacat tttgcagatg
     2041 gactcgatgg ccgccttggt gacccagcct tcctgctcct gcgcgatcat caaaagcggg
     2101 atgatcgccg attgctcgcg gcctttcggg tatttcttga tccactgctt cacggccgcc
     2161 gtgttcgcct tgttgaaggc gaagcttgct ggctggacgc tggcttctgc tagacggcga
     2221 acggacattt aacgatcgac ctcaccaaac acgatatcga gggagccgag gatggcggtg
     2281 acgtcggcaa gcatgtggcc gctgcaaaga aaatccatgg cctggagatg cgcgaagccc
     2341 ggcgctcgca acttgcagcg atagggcttg ttggtgccgt ccgacacgag atagacgccg
     2401 aactcgccct tcggcgcttc gaccgccgcg tagacttcgc ccgccggaac cttgtagcct
     2461 tcggtgtaaa gcttgaagtg atggatgaga gcttccatcg agcgcttcat ggcggcgcgc
     2521 ttcggcggca cgactttgcc gtcgaggttg gataccggac cggtgctttc cgtgcccagc
     2581 agccggtcga cacactggcg catgatccgc gccgactggc gcatctcttc catgcggatg
     2641 agataacggt catagcagtc gccgttcttg ccgatcggca cgtcgaattc catctcgtca
     2701 tagcattcgt agggctgcga cttgcgcaag tcccacgcag ccccggagcc gcgaaccatc
     2761 acgccggaaa aaccccacgc ccatgcatcg gccagcgaaa caacgccgat atcgacattg
     2821 cgctgcttga agatgcggtt cggcgtcaaa agcgcgtcca gatcgtcaag cgacttcagg
     2881 aacggatcga tccacctgcc gatatcttcg atcagttgct tcggcaaatc ctgatgcacg
     2941 ccgcccggac gaaaataagc cgcatgcatg cgtgagccgg aagcgcgctc gtaaaacacc
     3001 atcagctttt cgcgctcgac aaagccccat agcggcggcg tcagcgcgcc gatgtccagc
     3061 gcctgcgtcg tcacattgag gatgtgcgac atgatgcggc cgatttcgca atacagaacc
     3121 cggatcaact ggccgcgctt gggcacctcg atgccaagca gacgctcggc ggcgagcgcg
     3181 aaggcatgct cctggttcat tggcgcgaca tagtcgagcc ggtcgagata aggcacggcc
     3241 tgtagatagg tcttgtgctc gatcagcttt tcggtgccgc gatgcaacag gccaatatgc
     3301 ggatcgacac gggttacaag ttcgccatcc agctccagca cgagacgcaa aacgccatgc
     3361 gccgccggat gctgcggtcc aaaattgatg ttgaaattac gtacagaggt ttcagccatg
     3421 ccccgcctca gttcgccttg gctttttcat cgcccggcag cacgtaatcc gtgccttccc
     3481 agggcgagag aaaatcgaaa ttgcgaaatt cctgcttcaa ttccaccggc tcgtagatga
     3541 cgcgctttgc ctcgtcgtca tagcgcagtt cgacaaagcc ggtcagcggg aaatccttgc
     3601 gcagcggatg cccctcgaaa ccataatcgg tgaggatgcg ccgcaggtcc ggatgcccgg
     3661 taaacaggac gccgtaaagg tcgtaagttt cgcgctcgaa ccagtcggcg ccgatataga
     3721 cgccagtaat cgacggcacc agcgtatcct cgtccgcctg aaccttcacg cggatgcgcg
     3781 tattctgctg cggcgacagg aagtgataaa ccacctcgaa acgcatggca cgcgacggat
     3841 agtcggcgcc gcaaatgtcg atgatcgaaa tgaaccggca tttcggatcg tcgcgaagga
     3901 aagtcgccac gccgacaatg tcttgcgcct tcaccgtcag cgtcaattcg ccgaaggcga
     3961 caacgctttc caggatctcg gcatcgaccg tagcggcgat atgcccggcc aggtcgtcaa
     4021 gctgctcgct catgcgctca ccgttcaatc gtgccggtac ggcgaacctt cttctgcaac
     4081 agaagaaggc catacatcag cgcttccgcg ctcggcggac agccgggcac ataaatgtcc
     4141 accggcacga tgcggtcgca gccgcgcacc acggaatagg aatagtgata atagccgccg
     4201 ccattggcgc acgaccccat ggagatgacg tagcgcggct ccggcatctg gtcgtagacc
     4261 ttgcgcagcg ccggggccat cttgttggtc agcgtgccgg ccacgatcat cacgtccgac
     4321 tgacgcggcg aagcacgcgg cgcgatgccg aagcgctcgc tgtcgtagcg cggcatggcc
     4381 gaatggatca tctcgaccgc gcagcaagcc agcccgaagg tcatatacat cagcgagcct
     4441 gagcgcgccc acgtaatcag cgcctcggtc gaggtgacca gaaagccctt gtcggccagt
     4501 tcgtcattga tgccgaggaa gaacggatcg tcggccccga caggcttgcc ggtgctcgga
     4561 tcgagaatac ccttgggccg cggcgcaacc agcgtgccgg aactgtcgct caatcccatt
     4621 cgagcgctcc cttcttccat tcataggcga agccgatggt cagcaccgcc aggaatacca
     4681 tcatcgacca gaagcccagc atgccgatct tgctgaagga caccgcccac ggaaacagga
     4741 acgccacttc gagatcgaag atgatgaaca ggatggacac caggtagaag cggatatcga
     4801 acttcatgcg ggcatcgtcg aaagagttga acccgcactc ataggcggaa agcttttccg
     4861 gatcggggct gcggtaggcc accaggaagg gcgccgccaa cagcgccacg cctaccacca
     4921 gggcgacgcc gatgaaaaga acgatgggca gataggaact aatgagcgcg ttcatgctcc
     4981 ggctttccaa tggcggccag tccgcattga tttatagcaa cggcccgatt ggcgatagcg
     5041 tcatggtgac gctgaaccgg ttcaggctat gctgcaatgc ggcgagggtt agcgcagccc
     5101 tcccgtcgaa gcaagtcaaa ccttgttcaa aatacggtct tggaatggcc ttaccaccca
     5161 aactggttcg attggctccg gtttgatttc cttcattttt tactccactt ttgtctcctg
     5221 acaccgttac cggcgagagc ctgctattat cgatggaatc atcgacagcc gagatgtgag
     5281 aatttgccgg aatgagggaa aagatatcgc tgggccttgc tcgtcgtatc gcgctggccg
     5341 cgcaaggctt tgccgacccc agaccctccg tcacgcccga caggcggcac ctggcgcgcg
     5401 tcctttcacg gaccggcttg ttgcagatcg attccgtcag cgccgtggtg cgcgctcatt
     5461 atatgccgct ttattcgcgg ctcggcccct acccgctttc cctgctcgac aatgcggcgg
     5521 tctcacgaaa acgtgcgctc ttcgaatact gggcgcatga ggcttcttac ctgccggttg
     5581 aaacttatcc gctgatgcgc tggcgtatgg agcgcgcgca acgcggcgag gaaatgtatc
     5641 tcggcctggc gaaatggggc catgagcggc agcccttgat cgaggacatc tacaatcagg
     5701 tcgtggccgg cggcccgatt cccgcctcgt ccatcgaagg ccacaagggt tccggcggct
     5761 ggtggggctg gagcgaagca aagaacgcct ttgagtggct gttctgggcc ggccgcatca
     5821 ccacggccta ccgtcgaggc ttcgagcgtt tctacgacct gcccgagcgc gtgctgcccc
     5881 ggcatatact cgacctcccc acaccttcgc ctgaagatgc gcgccgccaa ttgctgcgca
     5941 tctcggcgcg cgcccacggt atcgccacgg ccagttccct gcgtgactat ttccgcctgt
     6001 cgccagccga catgaaggat cggctggaag agcttgtcga ggaaggcgag atttcgccgg
     6061 ttcgcgtcga aggctgggac aagccggctt acctgcacaa aagcgcgaaa atgccgcgcc
     6121 ggatcgagac ccgcgccttg cttgcgcctt ttgatccgct ggtgttcgag cgttcgcgca
     6181 ccgaacaatt gtttggtttc cgctaccgca tcgagattta tacgccggcc gacaagcgcc
     6241 aatatggcta ttatgtcctg ccgttcctgt tgaacgagcg catcgtcgcc cgtgtcgacc
     6301 tgaaggccga ccgaccggcc ggcatcctgc gcgtccatgc cgcctatgcc gaggccggcg
     6361 cgccggctga aacggccgcc gagcttttcg aggaactgaa gcagatgcag gcatggcttg
     6421 gcctcgaaac gatcgaggtg acgccggctg gcgatctcgg tccggctctg gccgacattg
     6481 ccctgccgca tcgccgcgtt gacacggccg ccaactaaag cctaaatccg cgcagacggt
     6541 ctcctccttt caaagggagg agtaataggg aatgcggtgc gagaccaaat ctcaaatccg
     6601 cggctgtccc cgcaactgta agcgaagagc caaggccgaa agccactggg aaaccgggaa
     6661 ggcggcacca aggcgatgac gcgcgagcca ggagacctgc cgtccaagat gaatatccga
     6721 tagcctggcg ggtttccggg catggagcgt gatgtgaacg acgacagtta tccgactgta
     6781 tccgcagaga tttcgaccgc cgtggacgcg acggtcatcg tttgctcgtc ctgccgcgat
     6841 gaaacaggtt ccgacgttta tccgcgcgcc ggcgaaaagc tggccgacga cgcccgccgc
     6901 gccgcgatgg gcgaaaatat ccacgtccgc acggtcgaat gcctcggcaa ctgcaagcgc
     6961 cggctcagcg ccgccatcct gcgcgacggc tgctggagct atgtcttcgg cgacctgacg
     7021 cccgacagcg gcgccgatct cattgccggc gcaaaactct ttgccggctc cgccgatggc
     7081 ctcattccgt ggcgcggtcg ccccgattgc ctcaagcgcg gcctcgtcgc ccgcatccct
     7141 ccccttagcc ttctgaagga ccgaccatga ccgcctccgt cacccgcgtt ccctgcaccg
     7201 tcatcaccgg ctttctcggc tccggcaaga ccacgctggt gcgccatttg ctggaaaatt
     7261 ccaaaggcaa gcggctcgcc ctcatcgtca acgagttcgg cgatatcggc gtcgacggcg
     7321 aaatcctcaa aggttgcggc atcgacacct gcccggagga aaacatcgtt gaactggcca
     7381 atggctgcat ctgttgcacc gttgccgacg acttcgttcc cgcactcgac cagatcttgt
     7441 cgctgacgcc gaaagtcgat cacatcctga tcgaaacctc cggcctcgcc ttgccgaagc
     7501 cgctggtcca ggcattccag tggcctaccg tcaaaagccg cgtcaccgtc gatggcgtaa
     7561 tcgccgtggt cgacggtccg gcgctcgccg ccggtcaggt cgcctccgac atggacgcgc
     7621 tggccgccca gcgcgccggc gatgacagcc tcgaccatga cgacccggtc gaggaattgt
     7681 tcgaggacca ggtcgcttgc gccgatctca tcatcctttc caagaaggac ctgatggacg
     7741 aggacggtgt cgcacgcgcc aacgcgatca tcggcaaaag ccgcgcccgc gccgtccaga
     7801 ccgtttcggt ttcgcacggc caggtggacc cgtcggtcct gcttggcctt ggcctcgcgg
     7861 tcgaggacga catcgaaaat cgctggagcc accatgatgg catgggcagc catgagcacg
     7921 atgatttcga cagcttcatc gtcgacgttc cctccatcgc caacccggat gaactggccg
     7981 cgcgcgtcgc cgatgcggcg gaacaggaaa acgtgctgcg cgtgaaaggc ttcgtcgagg
     8041 tcgccggcaa gccgatgcgg cttctggtcc aggcggtcgg cccgcgcgtc aaccattatt
     8101 acgaccgcgc atggacgccg gccgacgacc gccgttcgcg tctggtggtg atcggcctga
     8161 agggcttgaa ccggccggca atcgagaaaa tactggccgg ctgatagagc cgccgggcct
     8221 gcgatgcata ttctgaccac cacttccgcc tcgctcgacg atctcgccga gcctgtcgat
     8281 ctgcggcaga cgcccgccga tgtggtggcg ctgtccttta ccgacagcga cctggcgggc
     8341 cttgccgccg catggcaggc cgatgcggac agcatgccgt cgatgcggct ggcgccattg
     8401 cgcgatttgc gccatcccat gtccgtcgat ctatggatcg aaagcgtggc gcagcacgcc
     8461 aaggtcattc tggtgcgcat cctcggcggg tatgattggt ggcgctatgg ctgcgaccgg
     8521 ctctcggccg ttgcccggtc gcgcggcata aaactggcgc tgctgccggg cgaatgccgc
     8581 gaggaggatt tgcggctgat cgaggcttcc accctgcccc gcgccgagct tgacggcctg
     8641 ctcgattatt tccgggaagg cggccctgcc aatatgagtg cgctggtgca tcacctcgcg
     8701 cggcttgccg gaagggatac cactgtcgcc gaaccggtat cggtgccgaa ggcaggctat
     8761 tacgaaccgg gcgtgggtgt cgtcgaaacg cccgcgatgt acacaggccc tgtcgttgcg
     8821 atcctgtttt atcgttccat gctgctggct gcggatgtgg ctccaatcga tgccctgacc
     8881 gaggcgcttg ggcggcgcgg catcgccgcc ttgccgatct tcgtttcaag cctcaaagac
     8941 ccggcttcgc tggccttcgt ggaaaacgca ctcgcttgct tgcacccatc ggccatcgtc
     9001 accgctaccg cgttcgcctc tggtgcggag ccgggcagcc agactttgtt cgaccgcgcc
     9061 agcgtgccgg tcttccaggt catcgtggcg accacgcgcc gggatatctg gcaggaaaac
     9121 cagcgcggcc tcgccccttc cgatctggc
//
